LOCUS       DAZFQD010000001       114650 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.001, whole genome shotgun sequence.
ACCESSION   DAZFQD010000001 DAZFQD010000000
VERSION     DAZFQD010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 114650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 114650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..114650
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.001"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..209)
                     /locus_tag="ACHKTW_000001"
     CDS             complement(<1..209)
                     /locus_tag="ACHKTW_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFO6461097.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(280..2394)
                     /gene="fusA"
                     /locus_tag="ACHKTW_000002"
     CDS             complement(280..2394)
                     /gene="fusA"
                     /locus_tag="ACHKTW_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFO6461098.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRMGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="ACHKTW_000003"
     CDS             complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="ACHKTW_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFO6461099.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="ACHKTW_000004"
     CDS             complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="ACHKTW_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFO6461100.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="ACHKTW_000005"
     CDS             complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="ACHKTW_000005"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HFO6461101.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGQISNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="ACHKTW_000006"
     CDS             complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="ACHKTW_000006"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HFO6461102.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLSGQKPDAVLARDYIATFKLLGLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="ACHKTW_000007"
     CDS             complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="ACHKTW_000007"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HFO6461103.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIVEGHELSSVFFYREGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGIVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4598..5320)
                     /gene="yheO"
                     /locus_tag="ACHKTW_000008"
     CDS             complement(4598..5320)
                     /gene="yheO"
                     /locus_tag="ACHKTW_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO6461104.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="ACHKTW_000009"
     CDS             complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="ACHKTW_000009"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFO6461105.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKAAFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6520..6738
                     /gene="slyX"
                     /locus_tag="ACHKTW_000010"
     CDS             6520..6738
                     /gene="slyX"
                     /locus_tag="ACHKTW_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HFO6461106.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(6787..7377)
                     /gene="slyD"
                     /locus_tag="ACHKTW_000011"
     CDS             complement(6787..7377)
                     /gene="slyD"
                     /locus_tag="ACHKTW_000011"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFO6461107.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7472..7672)
                     /gene="yheV"
                     /locus_tag="ACHKTW_000012"
     CDS             complement(7472..7672)
                     /gene="yheV"
                     /locus_tag="ACHKTW_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative zinc ribbon protein"
                     /protein_id="HFO6461108.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVRKDEQVIGIFHPD"
     gene            complement(7682..9487)
                     /gene="kefB"
                     /locus_tag="ACHKTW_000013"
     CDS             complement(7682..9487)
                     /gene="kefB"
                     /locus_tag="ACHKTW_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
                     /protein_id="HFO6461109.1"
                     /translation="MEGSDFLLAGVLFLFAAVAAVPLASRLGIGAVLGYLLAGIAIGP
                     WGLGFISDVDEILHFSELGVVFLMFIIGLELNPSKLWQLRRSIFGVGAAQVLLSAALL
                     AGLLMLTDFAWQAAVVGGIGLAMSSTAMALQLMREKGMNRSESGQLGFSVLLFQDLAV
                     IPALALVPLLAGSADEHFDWMKIGMKVLAFVGMLIGGRYLLRPVFRFIAASGVREVFT
                     AATLLLVLGSALFMDALGLSMALGTFIAGVLLAESEYRHELETAIDPFKGLLLGLFFI
                     SVGMSLNLGVLYTHLLWVVISVVVLVAVKILVLYLLARLYGVRSSERMQFAGVLSQGG
                     EFAFVLFSTASSQRLFQGDQMALLLVTVTLSMMTTPLLMKLVDKWLSRQFNGLEEEDE
                     KPWVNDDKPQVIVVGFGRFGQVIGRLLMANKMRITVLERDISAVNLMRKYGYKVYYGD
                     ATQVDLLRSAGAEAAESIVITCNEPEDTMKLVEICQQHFPHLHILARARGRVEAHELL
                     QAGVTQFSRETFSSALELGRKTLVTLGMHPHQAQRAQLHFRRLDMRMLRELIPMHADT
                     VQISRAREARRELEEIFQREMQQERRQLDGWDEFE"
     gene            complement(9487..10038)
                     /gene="kefG"
                     /locus_tag="ACHKTW_000014"
     CDS             complement(9487..10038)
                     /gene="kefG"
                     /locus_tag="ACHKTW_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HFO6461110.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRSVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     QELASHARAYGDWLANPLSPGGR"
     gene            10169..12082
                     /gene="yheS"
                     /locus_tag="ACHKTW_000015"
     CDS             10169..12082
                     /gene="yheS"
                     /locus_tag="ACHKTW_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO6461111.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHGKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFQGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDDAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12082..13104
                     /gene="yheT"
                     /locus_tag="ACHKTW_000016"
     CDS             12082..13104
                     /gene="yheT"
                     /locus_tag="ACHKTW_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFO6461112.1"
                     /translation="MAQITTTDANEFSSSAEFIPMRGFSNCHLQTMLPRLFRRQVKFT
                     PYWQRLELPDGDFVDLAWSENPAQAQHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPPQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13098..13316
                     /gene="yheU"
                     /locus_tag="ACHKTW_000017"
     CDS             13098..13316
                     /gene="yheU"
                     /locus_tag="ACHKTW_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HFO6461113.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13370..14239
                     /gene="prkB"
                     /locus_tag="ACHKTW_000018"
     CDS             13370..14239
                     /gene="prkB"
                     /locus_tag="ACHKTW_000018"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008974 - phosphoribulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HFO6461114.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKID"
     gene            complement(14294..14698)
                     /gene="yhfA"
                     /locus_tag="ACHKTW_000019"
     CDS             complement(14294..14698)
                     /gene="yhfA"
                     /locus_tag="ACHKTW_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HFO6461115.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            15000..15632
                     /gene="crp"
                     /locus_tag="ACHKTW_000020"
     CDS             15000..15632
                     /gene="crp"
                     /locus_tag="ACHKTW_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HFO6461116.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            15683..17773
                     /gene="yhfK"
                     /locus_tag="ACHKTW_000021"
     CDS             15683..17773
                     /gene="yhfK"
                     /locus_tag="ACHKTW_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312236.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccS/YhfK family putative transporter"
                     /protein_id="HFO6461117.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKRLIIGASLFATCSLLTQLLLAKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTALHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPEGYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(17840..19060)
                     /gene="argD"
                     /locus_tag="ACHKTW_000022"
                     /gene_synonym="dapC"
     CDS             complement(17840..19060)
                     /gene="argD"
                     /locus_tag="ACHKTW_000022"
                     /gene_synonym="dapC"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HFO6461118.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGLGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYMHYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLSCAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPQFKGRARDFLYAGAGAGVMVLNAGPDVMRFAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(19146..19709)
                     /gene="pabA"
                     /locus_tag="ACHKTW_000023"
     CDS             complement(19146..19709)
                     /gene="pabA"
                     /locus_tag="ACHKTW_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HFO6461119.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHNGEGVFRGLANPLTVTRYHSLVVEPGSLPACFDVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHR"
     gene            complement(19741..20343)
                     /gene="fic"
                     /locus_tag="ACHKTW_000024"
     CDS             complement(19741..20343)
                     /gene="fic"
                     /locus_tag="ACHKTW_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HFO6461120.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIHQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSEAGESE"
     gene            complement(20333..20500)
                     /gene="yhfG"
                     /locus_tag="ACHKTW_000025"
     CDS             complement(20333..20500)
                     /gene="yhfG"
                     /locus_tag="ACHKTW_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HFO6461121.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRRHYER"
     gene            complement(20605..21177)
                     /gene="ppiA"
                     /locus_tag="ACHKTW_000026"
                     /gene_synonym="rotA"
     CDS             complement(20605..21177)
                     /gene="ppiA"
                     /locus_tag="ACHKTW_000026"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HFO6461122.1"
                     /translation="MFKSTLAAMAAVFALSALSPAAMAAKGDPHVLLTTSAGNIELEL
                     DKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKNEAD
                     NGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMDVAD
                     KISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            21448..22629
                     /gene="tsgA"
                     /locus_tag="ACHKTW_000027"
     CDS             21448..22629
                     /gene="tsgA"
                     /locus_tag="ACHKTW_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HFO6461123.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSAA
                     MFILGVVSGITMSIGTFLVTQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYIAIFILTFGCEFPALGKHAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPAHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            22891..25434
                     /gene="nirB"
                     /locus_tag="ACHKTW_000028"
     CDS             22891..25434
                     /gene="nirB"
                     /locus_tag="ACHKTW_000028"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HFO6461124.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            25431..25757
                     /gene="nirD"
                     /locus_tag="ACHKTW_000029"
     CDS             25431..25757
                     /gene="nirD"
                     /locus_tag="ACHKTW_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HFO6461125.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDEQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            25884..26690
                     /gene="nirC"
                     /locus_tag="ACHKTW_000030"
     CDS             25884..26690
                     /gene="nirC"
                     /locus_tag="ACHKTW_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HFO6461126.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGSISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            26709..28082
                     /gene="cysG"
                     /locus_tag="ACHKTW_000031"
     CDS             26709..28082
                     /gene="cysG"
                     /locus_tag="ACHKTW_000031"
                     /EC_number="1.3.1.76"
                     /EC_number="2.1.1.107"
                     /EC_number="4.99.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0043115 - precorrin-2 dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051266 - sirohydrochlorin ferrochelatase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siroheme synthase CysG"
                     /protein_id="HFO6461127.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDALNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMSERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
     gene            28329..28496
                     /gene="yhfL"
                     /locus_tag="ACHKTW_000032"
     CDS             28329..28496
                     /gene="yhfL"
                     /locus_tag="ACHKTW_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HFO6461128.1"
                     /translation="MNKFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISIS
                     KIIGGCGPTAQ"
     gene            28792..30129
                     /gene="frlA"
                     /locus_tag="ACHKTW_000033"
     CDS             28792..30129
                     /gene="frlA"
                     /locus_tag="ACHKTW_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
                     /protein_id="HFO6461129.1"
                     /translation="MGSQELQRKLGFWAVLAIAVGTTVGSGIFVSVGEVAKAAGTPWL
                     TVLAFVIGGLIVIPQMCVYAELSTAYPENGADYVYLKNAGSRPLAFLSGWASFWANDA
                     PSLSIMALAIVSNLGFLTPIDPLPGKFIAAGLIIAFMLLHLRSVEGGAAFQTLITIAK
                     IIPFTIVIGLGIFWFKAENFAAPTTTAIGATGSFMALLAGISATSWSYTGMASICYMT
                     GEIKNPGKTMPRALIGSCLLVLVLYTLLALVISGLMPFDKLANSETPISDALTWIPAL
                     GSTAGIFVAITAMIVILGSLSSCVMYQPRLEYAMAKDNLFFKCFGHVHPKYNTPDVSI
                     ILQGALGIFFIFVSDLTSLLGYFTLVMCFKNTLTFGSIIWCRKRDDYKPLWRTPAFGL
                     MTTLAIASSLILVASTFVWAPIPGLICAVIVIATGLPAYAFWAKRSRQLNALS"
     gene            30150..31172
                     /gene="frlB"
                     /locus_tag="ACHKTW_000034"
     CDS             30150..31172
                     /gene="frlB"
                     /locus_tag="ACHKTW_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HFO6461130.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHAIVEEMVKRDI
                     DRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGWEFCDNTPYRLDDRCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPNAEIGKIKNDLKQLPNALGHLVRTWEEKGRQLGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYAEISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            31222..32052
                     /gene="frlC"
                     /locus_tag="ACHKTW_000035"
     CDS             31222..32052
                     /gene="frlC"
                     /locus_tag="ACHKTW_000035"
                     /EC_number="5.1.3.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
                     /protein_id="HFO6461131.1"
                     /translation="MKTGMFTCGHQRLPIEHAFRDASELGYDGIEIWGGRPHAFAPDL
                     KAGGIKQIKALAQTYQMPIIGYTPETNGYPYNMMLGDEHMRRESLDMIKLAMDMAKEM
                     NAGYTLISAAHAGYLTPPNVIWGRLAENLSELCEYAENIGMDLILEPLTPYESNVVCN
                     ANDVLHALALVPSPRLFSMVDICAPYVQAEPVMSYFDKLGDKLRHLHIVDSDGASDTH
                     YIPGEGKMPLRELMRDIIDRGYDGYCTVELVTMYMNEPRLYACQALERFRALLPEDER
                     "
     gene            32049..32834
                     /gene="frlD"
                     /locus_tag="ACHKTW_000036"
     CDS             32049..32834
                     /gene="frlD"
                     /locus_tag="ACHKTW_000036"
                     /EC_number="2.7.1.218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HFO6461132.1"
                     /translation="MKTLATIGDNCVDIYPQLNKAFSGGNAVNVAVYCTRYGIQPGCI
                     TWVGDDDYGTKLKQDLARMGVDISHVHTKHGVTAQTQVELHDNDRVFGDYTEGVMADF
                     ALSEEDYAWLAQYDIVHAAIWGHAEDAFPQLHAAGKLTAFNFSDKWDSPLWQTLVPHL
                     DFAFASAPQEDETLRLKMKAIVARGAGTVIVTLGENGSIAWDGAQFWRQAPEPVTVID
                     TMGAGDSFIAGFLCGWSAGMTLPQAMAQGTACAAKTIQYHGAW"
     gene            32934..33665
                     /gene="frlR"
                     /locus_tag="ACHKTW_000037"
                     /gene_synonym="yhfR"
     CDS             32934..33665
                     /gene="frlR"
                     /locus_tag="ACHKTW_000037"
                     /gene_synonym="yhfR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417834.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator FrlR"
                     /protein_id="HFO6461133.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKATK
                     EKVIEQERVSAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
     gene            complement(33817..34902)
                     /gene="yhfS"
                     /locus_tag="ACHKTW_000038"
     CDS             complement(33817..34902)
                     /gene="yhfS"
                     /locus_tag="ACHKTW_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFO6461134.1"
                     /translation="MKTFPLQSLTIIEAQQKQFALVDSICRHFPGSEFLTGGDLGLTP
                     GLNQPRVTQRVEQVLADAFHAQAAALVQGAGTGAIRAGLAALLKPGQRLLVHDAPVYP
                     TTRVIIEQMGLTLITVDFNDLSALKQVVDEQQPDAALVQHTRQQPQDSYVLADVLATL
                     RAAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPEGVGAVVGDADVINRIRA
                     TLYSGGSQIQGAQALEVLRGLVFAPVMHAVQAGVSERLLALLNGGAVPEVKSAVIANA
                     QSKVLIVEFHQPIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQSE
                     HCAIRINPNRSGEETVLRILRESIASI"
     gene            complement(34914..36218)
                     /gene="yhfT"
                     /locus_tag="ACHKTW_000039"
     CDS             complement(34914..36218)
                     /gene="yhfT"
                     /locus_tag="ACHKTW_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HFO6461135.1"
                     /translation="MDLYIQIIVVACLTGMTSLLAHRSAAVFHDGIRPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLV
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPMVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            complement(36230..36583)
                     /gene="yhfU"
                     /locus_tag="ACHKTW_000040"
     CDS             complement(36230..36583)
                     /gene="yhfU"
                     /locus_tag="ACHKTW_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HFO6461136.1"
                     /translation="MKKIGVAGLQREQIKKTIEATAPGSFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            complement(36594..37472)
                     /gene="php"
                     /locus_tag="ACHKTW_000041"
     CDS             complement(36594..37472)
                     /gene="php"
                     /locus_tag="ACHKTW_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HFO6461137.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     TRGVRNVIEMTNRYMGRNAQFMLDVMRETGINVVACTGYYQDAFFPEHVATRSVQELA
                     QEMVDEIEQGIDGTDLKAGIIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLRQSGFSQ
                     ADVDVMLRENPSQFFQ"
     gene            complement(37469..38695)
                     /gene="yhfW"
                     /locus_tag="ACHKTW_000042"
     CDS             complement(37469..38695)
                     /gene="yhfW"
                     /locus_tag="ACHKTW_000042"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFO6461138.1"
                     /translation="MARFVVLVIDSFGVGAMKDVTLVRPQDAGANTCGHILSQLPHLQ
                     LPTLEKLGLINALGYAPGDMQPSDSATWGVAELQHEGGDTFMGHQEILGTRPLPPLRM
                     PFCDVIDRVEQALVSAGWQVERRGDELQFLWVNQAVAIGDNLEADLGQVYNITANLSV
                     ISFDDAIKIGRIVREQVQVGRVITFGGLLTDSQRILDAAESKEGRFIGINAPRSGAYD
                     NGFQVVHMGYGVDEKVQVPQKLYEAGVPTVLVGKVADIVNNPYGVSWQNLVDSQRIMD
                     ITLDEFNTHPTAFICINIQETDLAGHAEDVARYAERLQVVDRNLARLVEAMQPDDCLV
                     VMADHGNDPTIGHSHHTHEVVPVLVYQQGMIATQLGVRTTLSDVGATVCEFFRAPPPQ
                     NGRSFLSSLRFAGDTL"
     gene            complement(38695..39858)
                     /gene="yhfX"
                     /locus_tag="ACHKTW_000043"
     CDS             complement(38695..39858)
                     /gene="yhfX"
                     /locus_tag="ACHKTW_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
                     /protein_id="HFO6461139.1"
                     /translation="MFVEALKRQNPALISAALSLWQQGKIAPDSWVIDVDQVLENGKR
                     LIETARLYGIELYLMTKQFGRNPWLAEKLLALGYSGIVAVDYKEARVMRRAGLSVAHQ
                     GHLVQIPCHQVADAVEQGTDVITVFTLDKAREVSAAAVKAGRVQFVLLKVYSDDDFLY
                     PGQESGFVQHSLHEVVAEIKKLPGLHLAGLTHFPCLLWDEAAGKVLPTPNLHTLIQAR
                     DQLAKSGIALEQLNAPSATSCTSLPLLAEYGVTHAEPGHALTGTIPANQQGDQPERIA
                     MLWLSEISHHFRGDSYCYGGGYYRRGHAQHALVFTPENQKITETNLKTVDDSSIDYTL
                     PLAGEYPVSSAVVLCFRTQIFVTRSDVVLVSGIHRGEPKIVGRYDSLGNSLGA"
     gene            complement(39942..40304)
                     /gene="yhfY"
                     /locus_tag="ACHKTW_000044"
     CDS             complement(39942..40304)
                     /gene="yhfY"
                     /locus_tag="ACHKTW_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFO6461140.1"
                     /translation="METRLNLLCEAGVIDKDICKGMMQVVNVLETECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDNELLAELAQSSHWQAVVQLHQVLLKEFALEVNPCEE
                     GYLLANLYGLWMAANEEV"
     gene            complement(40321..41226)
                     /gene="yhfZ"
                     /locus_tag="ACHKTW_000045"
     CDS             complement(40321..41226)
                     /gene="yhfZ"
                     /locus_tag="ACHKTW_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator YhfZ"
                     /protein_id="HFO6461141.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLTQKGLCLALE
                     LGPHTYVGEHQLICRKGESANVKRVGLDNRSADQKIMTDVFFGGSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRVDDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            complement(41495..42499)
                     /gene="trpS"
                     /locus_tag="ACHKTW_000046"
     CDS             complement(41495..42499)
                     /gene="trpS"
                     /locus_tag="ACHKTW_000046"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFO6461142.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFDYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASVHASRTLKAVYEAIGFVAKP
                     "
     gene            complement(42492..43250)
                     /gene="gph"
                     /locus_tag="ACHKTW_000047"
     CDS             complement(42492..43250)
                     /gene="gph"
                     /locus_tag="ACHKTW_000047"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HFO6461143.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEE
                     RVITWIGNGADVLMERALTWARQERATQRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            complement(43243..43920)
                     /gene="rpe"
                     /locus_tag="ACHKTW_000048"
     CDS             complement(43243..43920)
                     /gene="rpe"
                     /locus_tag="ACHKTW_000048"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFO6461144.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            complement(43938..44774)
                     /gene="dam"
                     /locus_tag="ACHKTW_000049"
     CDS             complement(43938..44774)
                     /gene="dam"
                     /locus_tag="ACHKTW_000049"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HFO6461145.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMARADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            complement(44881..46167)
                     /gene="damX"
                     /locus_tag="ACHKTW_000050"
     CDS             complement(44881..46167)
                     /gene="damX"
                     /locus_tag="ACHKTW_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HFO6461146.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTSSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPAATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPATTRPARQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPAAPVASTKAPAATSAPAPKETATTAPVQTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            complement(46259..47347)
                     /gene="aroB"
                     /locus_tag="ACHKTW_000051"
     CDS             complement(46259..47347)
                     /gene="aroB"
                     /locus_tag="ACHKTW_000051"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFO6461147.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTNET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLKRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEIRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            complement(47404..47925)
                     /gene="aroK"
                     /locus_tag="ACHKTW_000052"
     CDS             complement(47404..47925)
                     /gene="aroK"
                     /locus_tag="ACHKTW_000052"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HFO6461148.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            complement(48326..49564)
                     /gene="hofQ"
                     /locus_tag="ACHKTW_000053"
     CDS             complement(48326..49564)
                     /gene="hofQ"
                     /locus_tag="ACHKTW_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HFO6461149.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEDNILSVHSIAWQNNNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            complement(49476..49880)
                     /gene="hofP"
                     /locus_tag="ACHKTW_000054"
     CDS             complement(49476..49880)
                     /gene="hofP"
                     /locus_tag="ACHKTW_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HFO6461150.1"
                     /translation="MRVKRWLLAGIALCLLTGMRDPFKPPEDLCRISELSQWRYQGMV
                     GRGERIIGVIKDGQKKWRRVQQNDVLENGWTILQLTPDVLTLGTGTNCEPPQWLWQRQ
                     GDTNEAMDSRTTVDADTRRTGGKAAKSDADGG"
     gene            complement(49870..50310)
                     /gene="hofO"
                     /locus_tag="ACHKTW_000055"
     CDS             complement(49870..50310)
                     /gene="hofO"
                     /locus_tag="ACHKTW_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HFO6461151.1"
                     /translation="MNMFFDWWFATSPRLRQLCWAVWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQQWAALYRLVDTTPFSEEKTLPFSPLDFQLSGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLETPHEG"
     gene            complement(50294..50833)
                     /gene="hofN"
                     /locus_tag="ACHKTW_000056"
     CDS             complement(50294..50833)
                     /gene="hofN"
                     /locus_tag="ACHKTW_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HFO6461152.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTG
                     SAEARIDAVLLQAEQQLARSLQITKPRLLEQQQLREQRSQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            complement(50833..51612)
                     /gene="hofM"
                     /locus_tag="ACHKTW_000057"
     CDS             complement(50833..51612)
                     /gene="hofM"
                     /locus_tag="ACHKTW_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HFO6461153.1"
                     /translation="MAFKIWQIGLHLQQQEAVAVAIVRGAKECFLQRWWRLPLENDII
                     KDGRIVDAQQLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRYRWGRKLAVGMTSAKELAAALSVDPES
                     VAICGEGGFDPWEAVSVRQPPLPPSGGDFAIALGLALGKAY"
     gene            51732..54284
                     /gene="mrcA"
                     /locus_tag="ACHKTW_000058"
     CDS             51732..54284
                     /gene="mrcA"
                     /locus_tag="ACHKTW_000058"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417855.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HFO6461154.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVATLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTLMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQQATAMLADGSTVALSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            complement(54449..55009)
                     /gene="nudE"
                     /locus_tag="ACHKTW_000059"
     CDS             complement(54449..55009)
                     /gene="nudE"
                     /locus_tag="ACHKTW_000059"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HFO6461155.1"
                     /translation="MSKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            55329..57464
                     /gene="igaA"
                     /locus_tag="ACHKTW_000060"
     CDS             55329..57464
                     /gene="igaA"
                     /locus_tag="ACHKTW_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HFO6461156.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTGASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIYTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMEIQAYYESCLNPQLITPSESLIE"
     gene            57529..58197
                     /gene="yrfG"
                     /locus_tag="ACHKTW_000061"
     CDS             57529..58197
                     /gene="yrfG"
                     /locus_tag="ACHKTW_000061"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HFO6461157.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            58208..58609
                     /gene="hslR"
                     /locus_tag="ACHKTW_000062"
     CDS             58208..58609
                     /gene="hslR"
                     /locus_tag="ACHKTW_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HFO6461158.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMALAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            58634..59512
                     /gene="hslO"
                     /locus_tag="ACHKTW_000063"
     CDS             58634..59512
                     /gene="hslO"
                     /locus_tag="ACHKTW_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFO6461159.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNAQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADPQVH"
     gene            complement(59647..61371)
                     /gene="yhgE"
                     /locus_tag="ACHKTW_000064"
     CDS             complement(59647..61371)
                     /gene="yhgE"
                     /locus_tag="ACHKTW_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HFO6461160.1"
                     /translation="MDNVELSPATRWGMIATGLLQGLVCYLLIAWLAGKNHSWIVYGV
                     PATVAFSSVLLFSVISFKQKRLWGWLALVFIATTGMSGWLKWQTDGMNPWRAEKAIWD
                     FGCYLLLMAMLLLPWIQQSLRICNGSSRYSYFYQSVWHNVLILLVIFLANGLTWLVLL
                     LWGELFKLVGIKFFNTLFFATDWFMYLTLGLVTALAVILARTQSRLIDSIQKLFTLIA
                     TGLLPLVSLLTLMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMAIVRDPQKASL
                     PWTGPLRCLIKTALLVAPLYVFVAAWALWLRVAQYGWTVDRLQGALAVLVLLVWSLGY
                     FVSIVWRNGQNPLVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSHMARYQSGKN
                     TPDQVTIYMLEQSGRYGRAALESLKSDAGFMKDPKRARDLLMALDGEQHLQEQVSEKV
                     LAENVLIAPGSVKPDATFWSALIQDRYNVMTCIEKDACVLVEQDLNSDGQAERILFAF
                     NDDRVIVYGFDSDRKEWDALDMSLLPNEITKEKLLTAAKDGKLGTRPKAWRDLTVDGE
                     TLEINLSK"
     gene            61750..63372
                     /gene="pckA"
                     /locus_tag="ACHKTW_000065"
     CDS             61750..63372
                     /gene="pckA"
                     /locus_tag="ACHKTW_000065"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HFO6461161.1"
                     /translation="MRVNNGLTPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            complement(63449..64801)
                     /gene="envZ"
                     /locus_tag="ACHKTW_000066"
     CDS             complement(63449..64801)
                     /gene="envZ"
                     /locus_tag="ACHKTW_000066"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HFO6461162.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(64798..65517)
                     /gene="ompR"
                     /locus_tag="ACHKTW_000067"
     CDS             complement(64798..65517)
                     /gene="ompR"
                     /locus_tag="ACHKTW_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HFO6461163.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            65745..66221
                     /gene="greB"
                     /locus_tag="ACHKTW_000068"
     CDS             65745..66221
                     /gene="greB"
                     /locus_tag="ACHKTW_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HFO6461164.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCMENLKIVDYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEASWYVNAIEYVKP"
     gene            66318..68639
                     /gene="yhgF"
                     /locus_tag="ACHKTW_000069"
     CDS             66318..68639
                     /gene="yhgF"
                     /locus_tag="ACHKTW_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFO6461165.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLAKAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYVDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     GVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFQNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNARRGGGNERPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            69077..69304
                     /gene="feoA"
                     /locus_tag="ACHKTW_000070"
     CDS             69077..69304
                     /gene="feoA"
                     /locus_tag="ACHKTW_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HFO6461166.1"
                     /translation="MQYTPDTAWKITGFSHEISPAYRQKLLSLGMLPGSSFNVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            69321..71642
                     /gene="feoB"
                     /locus_tag="ACHKTW_000071"
     CDS             69321..71642
                     /gene="feoB"
                     /locus_tag="ACHKTW_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HFO6461167.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDAALARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFDVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQYEEFNPAEFNLGEELFSAVDETWQSL
                     KDTFSLSVLMNPIEASKGDGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVVIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            71642..71878
                     /gene="feoC"
                     /locus_tag="ACHKTW_000072"
     CDS             71642..71878
                     /gene="feoC"
                     /locus_tag="ACHKTW_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HFO6461168.1"
                     /translation="MASLIQVRDLLALRGRMEATQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            72081..72959
                     /gene="rpnA"
                     /locus_tag="ACHKTW_000073"
     CDS             72081..72959
                     /gene="rpnA"
                     /locus_tag="ACHKTW_000073"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnA"
                     /protein_id="HFO6461169.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPETARDFLAFHLPAPIHALCDMKT
                     LKLESSSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVPLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQRDLMGLVEQMACLLSSGYANDRQIKGLFNYILQ
                     TGDAVRFNDFIDGVAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLTDI
                     MRFTGVSEEELAAASQ"
     gene            complement(72988..73758)
                     /gene="bioH"
                     /locus_tag="ACHKTW_000074"
     CDS             complement(72988..73758)
                     /gene="bioH"
                     /locus_tag="ACHKTW_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HFO6461170.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWCCIDEELSSHFTLHL
                     VDLPGFGRSRGFGALSLADMAEAVLQQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDEWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVSMPFLRLYGYLDGLVPRKVVPML
                     DKLWPHSESYIFAKAAHAPFISHPVEFCHLLVALKQRV"
     gene            73796..74479
                     /gene="gntX"
                     /locus_tag="ACHKTW_000075"
     CDS             73796..74479
                     /gene="gntX"
                     /locus_tag="ACHKTW_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HFO6461171.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRATRTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSPLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIVSVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAAAV
                     QVWCLCRTL"
     gene            74538..75113
                     /gene="nfuA"
                     /locus_tag="ACHKTW_000076"
     CDS             74538..75113
                     /gene="nfuA"
                     /locus_tag="ACHKTW_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HFO6461172.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            75474..76790
                     /gene="gntT"
                     /locus_tag="ACHKTW_000077"
     CDS             75474..76790
                     /gene="gntT"
                     /locus_tag="ACHKTW_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HFO6461173.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(76835..78919)
                     /gene="malQ"
                     /locus_tag="ACHKTW_000078"
     CDS             complement(76835..78919)
                     /gene="malQ"
                     /locus_tag="ACHKTW_000078"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HFO6461174.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWLLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMTAFRQFVAEQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYQNVDSPEVRQFC
                     EEHRNDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLETMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(78929..81322)
                     /gene="malP"
                     /locus_tag="ACHKTW_000079"
     CDS             complement(78929..81322)
                     /gene="malP"
                     /locus_tag="ACHKTW_000079"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HFO6461175.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVTKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLIDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLQKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VRLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENPQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            81934..84639
                     /gene="malT"
                     /locus_tag="ACHKTW_000080"
     CDS             81934..84639
                     /gene="malT"
                     /locus_tag="ACHKTW_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
                     /protein_id="HFO6461176.1"
                     /translation="MLIPSKLSRPVRLDHTVVRERLLAKLSGANNFRLALITSPAGYG
                     KTTLISQWAAGKNDIGWYSLDEGDNQQERFASYLIAAVQQATNGHCAICETMAQKRQY
                     ASLTSLFAQLFIELAEWHSPLYLVIDDYHLITNPVIHESMRFFIRHQPENLTLVVLSR
                     NLPQLGIANLRVRDQLLEIGSQQLAFTHQEAKQFFDCRLSSPIEAAESSRICDDVSGW
                     ATALQLIALSARQNTHSAHKSARRLAGINASHLSDYLVDEVLDNVDLATRHFLLKSAI
                     LRSMNDALITRVTGEENGQMRLEEIERQGLFLQRMDDTGEWFCYHPLFGNFLRQRCQW
                     ELAAELPEIHRAAAESWMAQGFPSEAIHHALAAGDALMLRDILLNHAWSLFNHSELSL
                     LEESLKALPWDSLLENPQLVLLQAWLMQSQHRYGEVNTLLARAEHEIKDIREGTMHAE
                     FNALRAQVAINDGNPDEAERLAKLALEELPPGWFYSRIVATSVLGEVLHCKGELTRSL
                     ALMQQTEQMARQHDVWHYALWSLIQQSEILFAQGFLQTAWETQEKAFQLINEQHLEQL
                     PMHEFLVRIRAQLLWAWARLDEAEASARSGMEVLSSYQPQQQLQCLAMLIQCSLARGD
                     LDNARSQLNRLENLLGNGKYHSDWISNANKVRVIYWQMTGDKAAAANWLRHTAKPEFA
                     NNHFLQGQWRNIARAQILLGEFEPAEIVLEELNENARSLRLMSDLNRNLLLLNQLYWQ
                     AGRKSDAQRVLLDALKLANRTGFISHFVIEGEAMAQQLRQLIQLNTLPELEQHRAQRI
                     LREINQHHRHKFAHFDENFVERLLNHPEVPELIRTSPLTQREWQVLGLIYSGYSNEQI
                     AGELEVAATTIKTHIRNLYQKLGVAHRQAAVQHAQKLLKMMGYGV"
     gene            complement(84682..85698)
                     /gene="rtcA"
                     /locus_tag="ACHKTW_000081"
     CDS             complement(84682..85698)
                     /gene="rtcA"
                     /locus_tag="ACHKTW_000081"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003963 - RNA-3'-phosphate cyclase
                     activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HFO6461177.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITSIRAGRAK
                     PGLLRQHLTAVKAAAEICRATVEGAELGSQRLVFRPGTVRGGEYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVASFNTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGSFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAAVGEYLADQLVLPMALAGAGEFKVAHPSCHLLTNIAVVERFLPVRFGLIETDGVTR
                     VSIE"
     gene            complement(85702..86928)
                     /gene="rtcB"
                     /locus_tag="ACHKTW_000082"
     CDS             complement(85702..86928)
                     /gene="rtcB"
                     /locus_tag="ACHKTW_000082"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HFO6461178.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAEDLPENLAELRQA
                     IEMAVPHGRTTGRCKRDKGAWENPPVNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIEICLDESDQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQDQLETLPSRD
                     LAYFMEGTEYFDDYLRAVAWAQLFASLNRDAMMENVVTALQSITQKTVRQPQTLAMEE
                     INCHHNYVQKEQHFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSD
                     LVEVIYTLRQVVCVKG"
     gene            87117..88715
                     /gene="rtcR"
                     /locus_tag="ACHKTW_000083"
     CDS             87117..88715
                     /gene="rtcR"
                     /locus_tag="ACHKTW_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
                     /protein_id="HFO6461179.1"
                     /translation="MRKTVAFGFVGTVLDYAGRGSQRWSKWRPTLCLCQQESLVIDRL
                     ELLHDTRSRSLFETLKRDIASVSPETEVVGVEIELHNPWDFEEVYACLHDFARGYEFQ
                     PEKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQSSPPRKKEQPRGAGEVTIIDLD
                     LSRYNAIASRFAEERQQTLDFLKSGIATRNPHFNRMIEQIEKVAIKSRAPILLNGPTG
                     AGKSFLARRIFELKQARHQFSGAFVEVNCATLRGDTAMSTLFGHVKGAFTGARESREG
                     LLRSANGGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDL
                     RQLVAEGKFREDLYARINLWTFTLPGLRQRQEDIEPNLDYEVERHATLTGDSVRFNTE
                     ARRAWLAFATSPQATWRGNFRELSASVTRMATFATSGRITLDVVEDEINRLRYNWQES
                     RPSALTALLGAEAENIDLFDRLQLEHVIALCRQAKSLSAAGRLLFDVSRQGKASVNDA
                     DRLRKYLARFGLTWEAVQDQHSSS"
     gene            complement(88697..89455)
                     /locus_tag="ACHKTW_000084"
     CDS             complement(88697..89455)
                     /locus_tag="ACHKTW_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HFO6461180.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(89472..90302)
                     /gene="glpG"
                     /locus_tag="ACHKTW_000085"
     CDS             complement(89472..90302)
                     /gene="glpG"
                     /locus_tag="ACHKTW_000085"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HFO6461181.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWQAGHTGSGLHYRRYPFFAALRERAGPVTWVVMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYVQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(90347..90673)
                     /gene="glpE"
                     /locus_tag="ACHKTW_000086"
     CDS             complement(90347..90673)
                     /gene="glpE"
                     /locus_tag="ACHKTW_000086"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HFO6461182.1"
                     /translation="MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            90863..92368
                     /gene="glpD"
                     /locus_tag="ACHKTW_000087"
     CDS             90863..92368
                     /gene="glpD"
                     /locus_tag="ACHKTW_000087"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HFO6461183.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDEGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNAHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYARTYGSNSELLLGNAGTVSDLGEDFGHEFYEAE
                     LKYLVDHEWVRRADDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
     gene            complement(92422..93027)
                     /locus_tag="ACHKTW_000088"
     CDS             complement(92422..93027)
                     /locus_tag="ACHKTW_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000589351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6461184.1"
                     /translation="MIKFRLYIPPVILGFVIVPLLVWPTVIVLAVLIFTLTFLAEIIF
                     SFPLLVVRISLQELQLELMVEYALFFSVMAGIGWQFSRRTPPELKNRLHCWLVFSPVY
                     FWLILSNFILYISPEKSALLENIRNFFLTFVWLPLNFSPFWPQPWTDFVGPISAQLGF
                     ALGYYCHWCRKNRNQRKKWGDWVTCLSLAILAQGPLFNYLQ"
     gene            complement(93030..93797)
                     /locus_tag="ACHKTW_000089"
     CDS             complement(93030..93797)
                     /locus_tag="ACHKTW_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001182130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6461185.1"
                     /translation="MQPSPAMQALIEQIYHTFRRYRVPQKFVVCCEYCLSQQEQKALR
                     STSLRAIPYSLINAWNSSPGPDPQNSDEVRYFLQRLLEFVAQGHFDNIHVVFSLRRIN
                     LANKENWRADERAILQRFACQYMTDWVSGDEAVELQYMLEMFFRADIALAPLLDAINS
                     VPGFWSTVSLACLLNRYCEDYIRDNQDDIDNAITTQINAWARNNHPLLKERARQAIEN
                     PPKQPEQGTQHQVWVDDWIIDECLCAMYDASSESPGK"
     gene            complement(93800..94510)
                     /locus_tag="ACHKTW_000090"
     CDS             complement(93800..94510)
                     /locus_tag="ACHKTW_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6461186.1"
                     /translation="MELVNTLFASLAGTDPFTDVDITIANCKSAYWDEGIVQQLINQA
                     LDEGEKFVGADGLEGLLRYDVTLNIGLTSSKVWPGFSLDTATISRLCACGADFGFDPY
                     ISDVPDVQCDLNTTNDVTVQFTAMLNPDERVIIAKRPLKKCDSWIEDVYIFQVFKDAW
                     KFHNNNSLRGFRDKQAELKLYTRHYSVENCAEESCRDCNSCIRPSFSLSRSALIRLNA
                     ANARFVYQPFTRDQRARG"
     gene            complement(94526..96034)
                     /locus_tag="ACHKTW_000091"
     CDS             complement(94526..96034)
                     /locus_tag="ACHKTW_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFO6461187.1"
                     /translation="MMQNRKWILTSLVMTFFGIPILAQFLAAVIAMLGVGLAGIIEVC
                     NIFITPTIYLLLNIFMLALGALMLFFSGRVWADDSAPEKREIAVWRQCLFLVPALLTL
                     GVWIIALHLADYQFRQMGAGWLADLMLPWLGVLLASLVGGEYWWLVIIPVGAHISFSL
                     GYGWPTRYPLTGTSGLRCRNSLLFILLMLGFVAGYQAYLYKQLNPGVGVRENIDTWAW
                     RPDKLNNQLTPLRGKPQIQFTQNWPRLDGATAAYPIYASAFYALSVLPEDFHEWEYLA
                     NSRTPEAYNKIVKGNADIIFVAQPSGGQKKRAEESGVTLIYTPFAREAFVFIVNVDNP
                     VNSLTEQQVRDIFSGAITNWRTVGGNDQEIQTWQRPEDSGSQTVMQSQVMKNVRMISP
                     QETEVASMMEGMIKVVAEYRNTNNAIGYTFRYYATQMNDDKNIKLLAINGIAPTAENI
                     RNGKYPYIVDAFMVTRENTTSETQKLLEWFLTPQGQSLVEDVGYVPMYKTLP"
     gene            complement(96159..98606)
                     /gene="glgP"
                     /locus_tag="ACHKTW_000092"
     CDS             complement(96159..98606)
                     /gene="glgP"
                     /locus_tag="ACHKTW_000092"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HFO6461188.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
     gene            complement(98625..100058)
                     /gene="glgA"
                     /locus_tag="ACHKTW_000093"
     CDS             complement(98625..100058)
                     /gene="glgA"
                     /locus_tag="ACHKTW_000093"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HFO6461189.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KRQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(100058..101353)
                     /gene="glgC"
                     /locus_tag="ACHKTW_000094"
     CDS             complement(100058..101353)
                     /gene="glgC"
                     /locus_tag="ACHKTW_000094"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HFO6461190.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(101371..103344)
                     /gene="glgX"
                     /locus_tag="ACHKTW_000095"
     CDS             complement(101371..103344)
                     /gene="glgX"
                     /locus_tag="ACHKTW_000095"
                     /EC_number="3.2.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HFO6461191.1"
                     /translation="MTQLAIGKPAPIGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGHSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIEG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPGGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGSLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLTWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITAVWQGPAHGLCVFQR"
     gene            complement(103341..105527)
                     /gene="glgB"
                     /locus_tag="ACHKTW_000096"
     CDS             complement(103341..105527)
                     /gene="glgB"
                     /locus_tag="ACHKTW_000096"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HFO6461192.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPVYRQYHHDKLT
                     FGILYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SMHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(105800..106903)
                     /gene="asd"
                     /locus_tag="ACHKTW_000097"
     CDS             complement(105800..106903)
                     /gene="asd"
                     /locus_tag="ACHKTW_000097"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFO6461193.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            107096..107689
                     /gene="yhgN"
                     /locus_tag="ACHKTW_000098"
     CDS             107096..107689
                     /gene="yhgN"
                     /locus_tag="ACHKTW_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HFO6461194.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(107746..109086)
                     /gene="gntU"
                     /locus_tag="ACHKTW_000099"
     CDS             complement(107746..109086)
                     /gene="gntU"
                     /locus_tag="ACHKTW_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015128 - gluconate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035429 - gluconate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HFO6461195.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILHETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGSATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(109090..109617)
                     /gene="gntK"
                     /locus_tag="ACHKTW_000100"
     CDS             complement(109090..109617)
                     /gene="gntK"
                     /locus_tag="ACHKTW_000100"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFO6461196.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDAAFAMQRTNKVSLIVCSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLMVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(109756..110751)
                     /gene="gntR"
                     /locus_tag="ACHKTW_000101"
     CDS             complement(109756..110751)
                     /gene="gntR"
                     /locus_tag="ACHKTW_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HFO6461197.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSKSPCLDIAV
                     GFDNFEAARQMTTAIIARGHRHIAYLGARLDERTIIKQKGYEQAMLDADLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(110975..111670)
                     /gene="yhhW"
                     /locus_tag="ACHKTW_000102"
     CDS             complement(110975..111670)
                     /gene="yhhW"
                     /locus_tag="ACHKTW_000102"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HFO6461198.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSFANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(111793..112830)
                     /gene="yhhX"
                     /locus_tag="ACHKTW_000103"
     CDS             complement(111793..112830)
                     /gene="yhhX"
                     /locus_tag="ACHKTW_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFO6461199.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKPFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADNSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAK"
     gene            113163..113651
                     /gene="yhhY"
                     /locus_tag="ACHKTW_000104"
     CDS             113163..113651
                     /gene="yhhY"
                     /locus_tag="ACHKTW_000104"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFO6461200.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQRPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKYGFEIEGTGKKYALRNGEYVDAYYMAR
                     VK"
     gene            113888..>114650
                     /gene="tssD"
                     /locus_tag="ACHKTW_000105"
     CDS             113888..>114650
                     /gene="tssD"
                     /locus_tag="ACHKTW_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein TssD"
                     /protein_id="HFO6461201.1"
                     /translation="MSNIVYLTVTGEQQGSISAGCGTSESTGNRWQSGHEDEIFTFSL
                     LNNINNTGLGSQFHGITFCKLIDKSTPLFINSINNNEQLFMGFDFYRINRFGRLEKYY
                     YIQLRGAFLSAIHHQIIENQLDTETITISYEFILCQHLIANTEFSYLALPENYNRLFL
                     PNSKNQTNNRFKTLNSKAIGRLLAAGGVYNGNIEGFRDTAEKLGGDAIKGYDQILNEK
                     TAGIAIATASILLTKRSNVDTYTEINSYLGKLRGQQ"
BASE COUNT        27768 a        31058 c        28777 g        27047 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcacgagc agcaccgccg taggttttag ccagtacggt ggtgattgca
      121 gcggtcagag tagttttacc gtggtcaacg tggccgatag taccaacgtt aacgtgcggt
      181 tttgtacgtt caaatttttc tttagacacg gctatattcc ttactatagt gctctcccct
      241 tcaggagaga gcacgggact ttggtattaa cccttaggct tatttaccac gggcttcaat
      301 tacggcctga gcaacgttac tcggcgcttc atcatacttc aggaattcca tagtgtatga
      361 tgcacgacct ttggtcagag aacgcagctg agttgcgtat ccgaacattt cagacagcgg
      421 tacttcagcg tggatcttaa cgccagtaac ttcagattcc tgacctttga gcataccacg
      481 acgacggctc aagtcaccga taacgtcacc ggtgttctct tccggagttt ctacttcaac
      541 cttcatgatc ggctcaagca gaactggttt cgctttctta aagccttctt taaaggcgat
      601 agaagcagcc agtttaaacg ccagttcaga ggagtcaacg tcatggtaag aaccgaagtg
      661 cagacgaata cccatgtcta ctaccgggta gcctgccagc ggacctgctt tcagctgttc
      721 ctggatacct ttatcaacgg ccgggatgta ttcgccaggg attacaccac ctttaatgtc
      781 gttgatgaac tcgtagcctt tcgggtttga acccggctcc agcgggtaca tgtcgataac
      841 aacatgacca tactgaccac gaccaccaga ctgtttcgcg tgtttacctt caacatcggt
      901 aactttctgg cggatagttt cacggtaagc aacctgcggt ttacctacgt tcgcttcaac
      961 gttgaattca cgcttcatac ggtcaacgat gatgtcgagg tgcagttcgc ccatacccgc
     1021 gataatggtc tggttagatt cttcgtcagt ccatacacgg aaagacgggt cttctttagc
     1081 cagacggccc agagccagac ccattttttc ctggtcagct ttggttttcg gttcaactgc
     1141 gatggagatt accggctcag ggaattccat acgttccaga atgatcggcg catccgggtc
     1201 acacagggtg tcaccagtgg ttacgtcttt cagaccgata gcagcagcga tgtcgcccgc
     1261 gcgaacttct ttgatctctt cacgtttgtt agcgtgcatc tgaacgatac gaccgaaacg
     1321 ctcacgtgca gctttcacgg agttcagtac ggtatcacca gagttaacca caccggagta
     1381 aacacggaag aaggtcaggt taccaacaaa cgggtcggta gcgattttga acgccagtgc
     1441 agagaacggc tcgtcatcac ttgcgtgacg ctcagccgga gtgtctttac cgtcgtccag
     1501 gataccgttg atcgcaggta cgtcaaccgg ggatggcagg taatcaatta ccgcatccag
     1561 catcgcctga acacctttgt tcttgaacgc agaaccacag gttaccagga tgatttcgtt
     1621 gttcagaacg cgctgacgca gagcaccttt gatttctgct tcagtcagtt cttcaccacc
     1681 caggtatttt tccatcagct cttcagaagc ttcagctgcg gattcgatca ggttctggtg
     1741 ccattcgtta gccagttcaa ccatgtctgc cgggatatct tcgtattcga aggttacgcc
     1801 ctggtcagcg tcgttccagt tgatagcttt cattttcacc aggtcaacaa caccggtgaa
     1861 atgttcttca gcaccaatcg ccagctgcag cggaaccggg ttcgcgccca gacgggtttt
     1921 gatctggtta acaactttca ggaagttcgc ccccatgcgg tccattttgt taacgaacgc
     1981 aatgcgcgga actttatatt tgtttgcctg acgccatacg gtttcagact gcggctgaac
     2041 accaccaact gcgcagtaaa ccattaccgc accatcgaga acacgcatgg aacgttctac
     2101 ttcgattgtg aagtcaacgt gccccggggt gtcgatgatg ttgatgcgat gcggctcata
     2161 ctgcttagcc ataccagacc agaatgcagt agtcgcagcg gaagtgatgg taataccacg
     2221 ttcctgctcc tgctccatcc agtccatggt tgcagcgccg tcatgaactt caccgatttt
     2281 atggtttaca ccggtgtaga acagaatacg ttcggtagta gtggttttac cggcgtcgat
     2341 gtgcgcactg ataccgatat tacggtagcg tgcgatgggt gttgtacgag ccatttgttt
     2401 cctcgtttat cttttaggcg ttcaatttaa gtagcccaaa gcgggctgct tactggaagc
     2461 gcccgcctgg tgactaaaac tccgaaggga ttaccaacgg tagtgtgcga acgccttgtt
     2521 ggcttcggcc atacggtgaa cgtcttcacg tttcttaact gcagtacctt tgttttctgc
     2581 agcatcagaa agttcgttcg ccaggcgcag agccatggat ttatcaccgc gtttacgagc
     2641 agcttcaacg atccaacgca ttgccagagc attacgacga accggacgga cttcaactgg
     2701 tacctgataa gtagaaccac caacgcggcg agacttaact tctacagtcg ggcgcacgtt
     2761 ttcgagagct acttcgaatg cttccagttc agatttacca gagcgctgag ccagggtctc
     2821 cagcgcgctg tatacgatag attcagcagt agatttttta ccatctacca tcaggatatt
     2881 tacaaattta gccagcagtt ctgatccgaa cttcggatcc ggcagaattt tacgctgacc
     2941 aatgacgcga cgacgtggca tggaaatact ccgttgttaa ttcaggattg tccaaaactc
     3001 tacgagttta gtttgacatt taagttaaaa cgtttggcct tacttaacgg agaaccatta
     3061 agccttagga cgcttcacgc catacttgga acgagcctgc ttacggtctt taacgccgga
     3121 gcagtcaagc gcaccacgta cggtgtggta acgaacaccc gggaggtctt taacacgacc
     3181 gccacggatc aggatcacgg agtgctcctg caggttgtga ccttcaccac cgatgtagga
     3241 agtcacttcg aaaccgttag tcagacgaac acggcatact ttacgcagcg cggagttcgg
     3301 ttttttagga gtggtagtat atacacgagt acatacgcca cgtttttgcg ggcatgcttc
     3361 cagcgcaggc acgttgcttt tcgcaacttt gcgagcacgt ggtttgcgta ccagctggtt
     3421 aactgttgcc attaaatagc tcctggtttt agcttttgct tcgtaaacac gtaataaaac
     3481 gtcctcacac aatatgagga cgccgaattt tagggcgatg ccgaaaaggt gtcaagaaat
     3541 atacaacgat cccgccatca ccaggccatc tggctggagt gcttaaccgt aagtctgacg
     3601 aaatcagtat agtcaatggg aatgatgtcg ttcgaaattt gaccagtcaa accgcgggca
     3661 ataaggtctt cgttcagggc atagacctta atgggggcat tacgcagact ttcaaggtag
     3721 cggttaccgt caactgcggc agttacgcca tcttgcaata atagcagttc gtctccttca
     3781 ctgagcagac gcagcagcgc agcaaaatcc gtcagccagg gtgaacgatg taacgtgtgc
     3841 agcataaacg cctcaaaacc tcaaaataac atcgtagttg gcgagttcgc ggcgtaaggc
     3901 atctgcttcg agcggcgtgg cttcgacaac aaagggtgtc tgcggatcta acccgcgttc
     3961 gcgcagtgaa gccgcacaaa cccagcactg ttcaatgtcg tacagaccca acaatttaaa
     4021 agtggcaatg taatcacgcg ccagcactgc atcgggcttt tgtcctgaca gcagctgaaa
     4081 aacgccatca gcaataaaga agacggccag atcgtcagtt aatgcggaag ttgccagtaa
     4141 agcatctaaa ccttcccgac cagcagctgt accatgaggt gcagtagaaa aaacaaacgc
     4201 aattcgtttc atcagaactg taccaccctg tcacaggtca gcgaggcttc cgccagcgcg
     4261 ccaagtccgc ttaaggtaaa tcccggctga aggtttgacg aagccagccc cagccttcct
     4321 gcttcagttt catcgacgat accgcggcgt aacgctgccg ctacgcagat attcagcgcc
     4381 acaccatgtt gcgcattcaa ctgttgccag ccgcgcacaa ggtcaaattc gtcacttgcc
     4441 ggtgaggtca attggttagc gttatagacc ccttcccgat agaaaaagac gctgcttaac
     4501 tcatggcctt ctactatcag cgcctgcgca aactgaaaag cactactcgc ctgttgcgta
     4561 ccgtatgctg gcccggtcac cacgatggca aaacgcatta cttatcttgc ccctggaaat
     4621 caccgctttt gaactggcgg atgtagagat agacagtgtg tttggagatg ttcaggcggt
     4681 cagcaacctg gttgatcgcg tctttaatat cgaagatccc tttctcgtag agattcagca
     4741 cgatctgacg atttttggcg ttattagaaa cattgcgatc ggcattcact tcttcgatgg
     4801 tgaactccag cgtttgggta accagatctt caacagaaga ggcaaagttg acgcttgaac
     4861 cgacatccgg ggtttctggc ggcacaaaag tgctcataat ctgcgagaag ggaacatcaa
     4921 gattcatatt gatgcacagc agaccaatta cacgctgttc gcggttacga atcgcgatag
     4981 tcagggactt cattaatacg ccgcttttgg cgcgagtaaa gtagcattta gaaacgctgc
     5041 tatccgctcc cgtcatatcg tgcagcatac gtagcgccag gtcagtaatt ggcgaaccaa
     5101 tcttccggcc tgtatgttca ccgttagcaa tgcgaatggc tgaacatttt agatcctgca
     5161 aagagtgcaa aacgatttca cagtgggagc caataagcat cgctaacccg tccaccaccg
     5221 cttcgtagga tttcagaata tcaaaatcgg tctggtcgaa aggacgttga tccagtaaat
     5281 ccaactcact ggtttcgttg gttaaaagcg acctggacat gaaaaaaaac actccttttc
     5341 aggagcctgt cgttaacttt tcagggcagg ctcattaatg atgcgggtaa ctaaattaat
     5401 acagcggagg ttccgctttc cagcactaat tatatccggc ctgtaataaa aaaaccgccg
     5461 cctggtcagg cggcggttct taatgcttat tttttagcag aatctgcggc tttcgcatca
     5521 gcttccggct ttgcatcagc cttcggcgct ggtttcacgt ccaacagctc tacgtcaaac
     5581 accagggtag aattcggtgg gatccccgga acacccgctt tgccgtaagc cagttctggt
     5641 ggaataacca gtttgatctt accgcctttc ttgatgttct tcagaccttc tgtccaaccc
     5701 gggataacac cgtccagacg gaaagagagc ggttcaccac gagtgtaaga gttgtcgaac
     5761 tctttaccgt cgatcagagt acctttgtag ttcacgacaa cagtatcgct gtctttcggt
     5821 gcttcgcctt taccggcttc tactacctga taaaccagac cagttgaaga ggttttcaca
     5881 cctttctctt tggcaaattt ctcgcggtac tctttacctt ttgcttcgtt atcagccgcg
     5941 tctttttcca tcttcgcctg agcagaagac ttcacgcgag cttcgaatgc ttgcagagtc
     6001 tgttcgatct cttggtcgga gagtttgctc ttatcagcaa atgcatcctg aacaccagcg
     6061 atcagctgat ctttatccag tttgatgccc agtttttctt gttcttttag agagttttcc
     6121 atgtaacgac ccagcgaggc acccagtgca taagctgact tctgatcgtc atttttgaac
     6181 gctgctttgc tgtcagcagt tgtagcaggt tttgcagctt cagcagcaaa agtgattggt
     6241 gcatgcaggg caacggccat tgtggtcgcc agcagcgtta ctttaaacag tgatttcatc
     6301 catatctcca ggatcggggc atctcacccc agggttaact attatcagaa gggtactata
     6361 aagcgttgtc gaacaaatct acatacagac acgccctatt atcatctatt ttcagactct
     6421 ttttgtttaa attagtttcg atgaccgcga aatgagtgct gtctcgggca gcaaagttaa
     6481 gtagaatccg cggcaaccat tcgacaaaag aggtgaatca tgcaggattt atcattggaa
     6541 gcacgcctgg cagagctgga gagccgactg gcttttcagg aaatcaccat tgaagaactg
     6601 aacgtcacgg tgaccgctca tgaaatggag atggcgaaac tgcgcgatca tctgcgtctg
     6661 ctgactgaga agttaaaagc cagccagccg tcgaacatcg cgtcgcaggc tgaagaaacg
     6721 ccaccgccgc attattgagg cgaaaaaaaa gcggggaatc cccgcttttt tgtcaccttt
     6781 tcggtattag tggcaaccac aaccgccgtt gcctttaccg ccacagcagc cttcgccacc
     6841 gtgttcatga ccgtgatcgt ggccatgacc gccgcagcaa ccgtcgtggt cgtgatcgtg
     6901 gtggtgatcg tgcgcgccgt gaacgtggcc atgagccagt tcttcttcgg tcgcttcgcg
     6961 aatcgccaca acttcaacgt tgaatttcag gttctgaccg gccagcatgt ggttaccatc
     7021 aaccacgacg tgatcgtctt caaccgcagt gatttcaacc ggtaccggac cctggtcagt
     7081 ttcagccagg aaacgcatac ctacctgcag ttcatcaacg cccataaata cgtctttagg
     7141 aacacgttgc accaggtttt cgtcgtactg accgtaagcg tcgttcgcgc caacagcgac
     7201 atcaaatttg tcgccaactt catgaccttc cagcgccgtt tccaggccag agatcaggga
     7261 accgtgacca tgcaggtagt ccagcggcgc actcaccgga gactcatcaa ccaacacacc
     7321 gtcttctgta cgtacctgat aggccaggct gaccaccagg tcttttgcta ctttcatgat
     7381 atctcctgag catgggaaga atagtggcgc agattgtagc ggaattctgc agccgtgtac
     7441 tcactagctt aaaaaaactc ggcgcatatc gctagtccgg atgaaaaatc ccgatcactt
     7501 gctcatcttt gcgaacgtga tcgcgggctt ctttgtctgc ttctcgcatc tggtgtccgc
     7561 acttaacgca ttcaacaata tcaatattat tttcgcgcca catcgccatt gaatcctgcg
     7621 cctgacaggc cgggcatttt gcgcccgcaa taaaacgttt tcggattgcc atctttaccc
     7681 tctactcaaa ttcatcccag ccgtccagct ggcgtcgttc ttgttgcatt tcacgttgga
     7741 aaatctcttc cagttcgcgt cgggcttccc tggcgcgaga aatttgtacg gtatcagcat
     7801 gcatcgggat taactcccgc agcattcgca tatccaggcg gcgaaaatgc agttgcgccc
     7861 gctgtgcctg atgcggatgc atgccaagcg tgaccagcgt cttgcgcccc agctctaacg
     7921 cactggagaa tgtttcacgg gaaaactgcg tcacccctgc ctgtaataac tcatgcgctt
     7981 ccacacgtcc gcgcgctcgc gcaagaatat gcaaatgcgg aaagtgctgc tggcatattt
     8041 ccaccagctt catggtgtct tccggctcgt tacaggtaat gacgatagac tcagcggcct
     8101 ctgcacccgc agaacgtaaa agatcgacct gcgtggcgtc gccgtaataa actttgtagc
     8161 catatttacg catcaggtta acggcgctga tatcccgctc cagcacggta atgcgcattt
     8221 tatttgccat cagcaaacga ccaatcacct gaccaaaacg cccgaagccc acgacaatga
     8281 cctggggttt atcatcgttg acccacggtt tttcgtcttc ttcttccagt ccgttaaact
     8341 ggcgggatag ccatttatcc accagcttca tcagcagcgg cgtggtcatc atggaaagcg
     8401 tcaccgtcac cagcaacagc gccatctggt cgccctggaa taagcgttgt gaagaagcgg
     8461 tagaaaagag gacaaaggca aactcaccgc cctgactcaa cacgccagca aactgcatcc
     8521 gctctgagct acgcacgcca tacaatcgcg ccagcagata caacacgaga attttcaccg
     8581 ccaccagcac aaccacgctt atcactaccc acaacagatg ggtataaagc accccgaggt
     8641 tgagcgacat gccgacagag ataaagaaca aaccgagcag caagcctttg aagggatcga
     8701 tagccgtttc cagttcatgg cgatattcac tttccgccag cagcacgccc gcaataaacg
     8761 taccgagcgc catcgacagc cccagcgcgt ccataaacaa tgcggaaccc aacaccaaca
     8821 gcagcgtcgc ggcggtgaac acttcccgca cgccagaagc tgcaataaaa cggaataccg
     8881 gacgcagtaa atagcgcccg ccaatcagca tgccgacaaa cgccagcacc ttcatgccga
     8941 tcttcatcca gtcgaaatgt tcgtctgccg aacccgccaa caacggcact aacgccagtg
     9001 ccgggatcac cgccagatcc tgaaacagca gaaccgaaaa cccgagctgc ccggattcgc
     9061 tgcgattcat ccctttctca cgcatcaatt gcaacgccat tgcagttgaa gacatcgcaa
     9121 ggccaatgcc accgacaacc gccgcctgcc aggcgaaatc cgtcagcatc aataatcccg
     9181 ccagcaacgc cgcgcttaac agcacctgtg ccgcgcctac gccaaaaatc gaacgccgca
     9241 gttgccaaag tttggaggga ttcaactcaa gaccgatgat aaacatcagg aataccacgc
     9301 cgagttccga aaagtggagg atctcatcga cgtcgctaat aaaccccagc ccccatgggc
     9361 caattgcaat ccctgccagc aaatatccca acacagcgcc aatacccagc cgcgatgcca
     9421 gcggcaccgc agccaccgcc gcgaagagaa acagcactcc tgcgagtaaa aaatcggaac
     9481 cttccatcag cggcctcctg gagacagcgg atttgccagc cagtcaccgt aggctctggc
     9541 gtgactcgcc agctcctgtg cgctttgccg tctcgcccag taaataatga tgggacttaa
     9601 ccaatgcatc cggcacatgc ccgccgccag ttcaaaggga cgcagaacat cgctcatcgg
     9661 gtagcgattc aatgcgtcat aacggtaagc actttccggc tcgccggtgg taatcacgct
     9721 acgccagtac tttcccgcca gttggtttcc tcccggcccg ctggcaaaac cacgacttaa
     9781 tacccggtcc agccactctt tcagtagcgc cgggcagcta taggtataaa gaggatgctg
     9841 aaagacaatc acctcgtgct cgcgcagtaa tgcctgctca cgggggatat caataaaaaa
     9901 atcgggatag tgcgcataaa ggtcgtgcac ggtaacattg ctgagctgcg tggccggttt
     9961 aagcagtacc cggtttgcca ccgagtcctg agattccgga tgggcataca gcagcaaaac
    10021 tttcgctggc tgagacatca tccccctccc ggtatggttt ttgtgtatag tcgctgtttt
    10081 gggctaccat tgcgcccggt gcggcagctc gcccatacat tacattatca taatgataag
    10141 ttaacatagt ctgaacatac ggcaccttat gattgttttc tcctcgttac aaattcgtcg
    10201 cggcgtgcgc gtcctgctgg ataatgccac cgccaccatc aaccctgggc agaaagtcgg
    10261 cctggtgggt aaaaacggct gtggtaaatc taccctgctg gcattgctga aaaatgaaat
    10321 cagcgccgac ggcggcagct acacctttcc gggaagctgg caactggcgt gggtgaatca
    10381 ggaaacgccg gcgttaccgc aagcggcgct ggaatatgtc attgacggcg accgtgaata
    10441 tcgtcaacta gaagcgcagc tacacgacgc caacgaacgt aacgacgggc acgccattgc
    10501 gaccattcat ggcaagctgg atgctattga cgcatggagt attcgctccc gtgccgccag
    10561 cctgctgcac ggcctcggtt tcagcaatga acaactggag cgcccggtaa gtgatttctc
    10621 cggtggctgg cgtatgcgtc ttaaccttgc ccaggcgctg atttgccgtt cagacttgct
    10681 gctgctcgac gaaccgacta accacctcga tctcgatgcc gttatctggc tggaaaaatg
    10741 gttgaagagc tatcagggca cgctgatcct gatctctcac gaccgcgact tcctcgatcc
    10801 gatcgttgat aaaattattc atatcgaaca acaaagcatg ttcgagtaca ccggcaacta
    10861 cagttcgttt gaagtacagc gcgccacccg tctggcgcag caacaagcga tgtatgaaag
    10921 ccagcaggaa cgcgtagcgc atctgcaaag ttatatcgac cgtttccgtg ccaaagccac
    10981 caaagcgaag caggcccaga gccgcattaa gatgctcgag cgtatggagc tgattgcccc
    11041 cgcgcacgtc gacaacccgt tccgctttag cttccgcgcg ccggaaagcc tgccaaatcc
    11101 gttactgaag atggaaaaag tcagcgcggg ctatggcgat cgcattattc tcgactcgat
    11161 taaactgaac ctggtgcccg gctcgcgcat tggtctgtta ggccgcaacg gcgcgggtaa
    11221 atcgacatta atcaaactgt tagccggtga acttgcgcca gtcagcggtg aaattggtct
    11281 ggcgaaaggg atcaagctcg gctacttcgc ccagcatcaa cttgaatacc tgcgcgccga
    11341 cgaatcacct attcaacatc tggcacgttt agcgccgcag gagctggaac aaaaactgcg
    11401 tgactacctc ggcggctttg gtttccaggg cgataaagta accgaagaaa cgcgccgctt
    11461 ctcaggtggg gaaaaagccc gcctggtgct ggcattaatt gtctggcagc gtccgaatct
    11521 gctgctgctc gacgaaccga ctaaccacct tgacctcgac atgcgtcagg cactcaccga
    11581 agcattaatc gagttcgaag gcgcgctggt tgtcgtctcg cacgaccgtc atttgctgcg
    11641 ttccaccact gacgacctct acctggttca cgatcgtaaa gtcgaaccgt tcgacggcga
    11701 tctggaagat tatcaacagt ggttgagcga cgtacaaaag caggaaaacc agaccgacga
    11761 cgcgccaaaa gagaatgcga acagcgccca ggcacgtaaa gatcagaagc gtcgggaagc
    11821 ggagctgcgt gcgcaaaccc agccactgcg taaagagatt gcccgtctgg aaaaagagat
    11881 ggagaagctg aacgcgcaac tggcgcaggc ggaagagaaa ctcggcgaca gcgaactgta
    11941 tgaccagagc cgtaaagcgg agttgaccgc ctgcctgcaa cagcaagcca gcgccaaatc
    12001 cggcctggaa gagtgcgaaa tggcgtggct ggaagcccag gagcagcttg agcagatgct
    12061 gctggaaggc caaagcaact gatggcgcag ataacgacga ccgatgccaa tgaattcagc
    12121 agcagtgctg aattcatccc catgcgcggc tttagcaact gccatctaca aactatgctg
    12181 ccgcgtctgt ttcgtcgtca ggtgaaattc accccgtact ggcagcggct ggagttgccc
    12241 gacggcgatt ttgtcgatct tgcgtggagt gaaaaccctg cacaggcgca acataaacct
    12301 cgtctggtgg tgtttcacgg gctggaaggc agtctcaata gcccttacgc ccacggtctg
    12361 gttgaggcgg cgcaaaagcg cggctggctg ggcgtggtga tgcattttcg cggatgcagc
    12421 ggtgaaccaa accgtatgca ccgcatttac cattcgggcg aaaccgaaga cgccagttgg
    12481 tttttacgct ggctgcaacg cgaatttggt catgcgccaa cggctgccgt cggctattcg
    12541 ctcggcggta atatgctggc ctgtttgctg gcaaaagaag gcaatgatct ccctgttgat
    12601 gcggcggtga ttgtctctgc gccgtttatg ctggaagcct gtagctatca tatggaaaag
    12661 ggcttttccc gcgtttatca gcgttacttg ctgaacctgt taaaagccaa tgccgcacgc
    12721 aagctggcag cctaccccgg aacactgccg attaatctcg cgcagttaaa atcggtacgt
    12781 cgcatccgtg aatttgacga tctaatcacc gccagaattc acggctacgc tgacgctatc
    12841 gactattatc gtcagtgtag cgccatgccg atgctgaacc ggatcgccaa acctacgctg
    12901 attattcacg ccaaagacga tccgtttatg gatcatcagg tgatcccgaa accggaaagt
    12961 ctccccccgc aggttgagta tcaactgact gaacatggcg gtcatgttgg ctttattggc
    13021 ggtacattac ttcatccgca aatgtggctg gagtcacgca ttcctgactg gttaacaacg
    13081 tatctggagg cgaaatcatg ttgattccgt ggcaagacct ctcccccgaa acgctggaaa
    13141 atttaattga aagctttgtg ttacgtgaag gcaccgatta tggtgaacat gagcgtacac
    13201 ttgaacagaa agtcgccgac gtcaaacgcc agctacagtg cggagaagcg gtgctggtat
    13261 ggtcggagct gcacgaaacg gtcaatatca tgccgcgcag tcagtttcgc gaataacctg
    13321 caaccgtcgg cggacgagat aataatgata gctaagtcat ggagttacta tgtctgccaa
    13381 acatccggtc attgcggtaa caggatccag cggcgcgggg accaccacca ccagcctcgc
    13441 gtttcgtaaa atattcgcgc agttaaatct gcatgcagct gaggtggaag gcgacagttt
    13501 tcaccgttac acccgcccgg aaatggacat ggcgatccgc aaagcgcgcg acgccgggcg
    13561 gcatatcagc tacttcggcc ccgaagcgaa cgacttcggc ctgctggaac aaaccttcat
    13621 tgaatacggt cagagcggca aagggaaatc tcgcaaatat ctgcatacct acgacgaagc
    13681 cgtaccctgg aatcaggtac cggggacatt caccccctgg caacctttac cggaacccac
    13741 tgatgtactg ttttatgaag gtttacacgg cggcgtagtc acgccacagc ataacgttgc
    13801 gcagcatgtg gacttactgg tcggcgtggt gcctatcgtt aaccttgagt ggattcaaaa
    13861 actgatccgc gacaccagcg agcgcgggca ctcacgagaa gcagtgatgg actcagtagt
    13921 gcgttcaatg gaagactata tcaactacat cacaccgcag ttttcccgca cccatcttaa
    13981 cttccagcgc gttcccaccg tcgacacttc aaacccgttc gcggcaaaag gtatcccgtc
    14041 gctcgatgaa agctttgtgg tgatccattt tcgtaatctg gaagggatcg atttcccctg
    14101 gctgctggcg atgttgcaag gctcattcat ttcccacatc aatacgttag tggtaccggg
    14161 cggcaaaatg ggtctggcaa tggaattaat tatgctgccg ctggtgcaac gattgatgga
    14221 aggaaagaaa atcgactaac tctgctatta cgccggataa aatactatcc ggcttcacaa
    14281 cgggatagtt aaatcacgcg gcaaccactt catacgagtg agtaatattc accgcttttt
    14341 ccagcatcaa cgccactgaa caatatttct cagcagagag atcaaccgca cgcgcaaccg
    14401 ctgcgtcttt caggtcgcga ccggtgacga taaaatgcag attaatgtgc gtaaacaggc
    14461 gtggtgcctc ttcgcggcgt tcagaggtca attttacttc acaatcgacc acatcctgac
    14521 gccctttttg caggatcgaa accacatcga tggcactgca accacccgcc gccatcaaca
    14581 ccatttccat cggacttggt gctttatcgc ctgagttgcc gtccattaaa atctggtgac
    14641 cagaggcgga ttcgcccaga aaagttaacc cttcgaccca cttcactcgc gcttgcattt
    14701 ttgctactcc actgcgtcaa ttttcctgac agagtacgcg tactaaccaa atcgcgcaac
    14761 ggaaggcgac ctgggtcatg ctgaagcgag acaccaggag acacaaagcg aaagctatgc
    14821 taaaacagtc aggatgctac agtaatacat tgatgtactg catgtatgca aaggacgtca
    14881 cattaccgtg cagtacagtt gatagcccct tcccaggtag cgggaagcat atttcggcaa
    14941 tccagagaca gcggcgttat ctggctctgg agaaagctta taacagagga taaccgcgca
    15001 tggtgcttgg caaaccgcaa acagacccga ctctcgaatg gttcttgtct cattgccaca
    15061 ttcataagta cccatccaag agcacgctta ttcaccaggg tgaaaaagcg gaaacgctgt
    15121 actacatcgt taaaggctct gtggcagtgc tgatcaaaga cgaagagggt aaagaaatga
    15181 tcctctccta tctgaatcag ggtgatttta ttggcgaact gggcctgttt gaagagggcc
    15241 aggaacgtag cgcatgggta cgtgcgaaaa ccgcctgtga agtggctgaa atttcgtaca
    15301 aaaaatttcg ccaattgatt caggtaaacc cggacattct gatgcgtctg tctgcacaga
    15361 tggcgcgtcg tctgcaagtc acttcagaga aagtgggcaa cctggcgttc ctcgacgtga
    15421 cgggccgcat tgcacagact ctgctgaacc tggcaaaaca accagatgct atgactcacc
    15481 cggacggtat gcaaatcaaa attacccgtc aggaaatcgg tcagattgtc ggctgttctc
    15541 gtgaaaccgt gggacgcatt ctgaagatgc tggaagatca gaacctgatc tccgcacacg
    15601 gtaaaaccat cgtcgtttac ggcactcgtt aatcccgtcg gagtggcgcg ttacctggta
    15661 gcgcgccatt ttgtttcccc cgatgtggcg cagactgatt tatcaccccg atatcaacta
    15721 tgcacttcga caaacgctgg tgctatgttt gcccgtggcc gttgggttaa tgcttggcga
    15781 attacgattc ggtctgctct tctctctcgt tcctgcctgt tgcaatattg cgggccttga
    15841 tacgcctcat aaacgttttt tcaaacgctt aatcattggt gcgtcgctgt ttgccacctg
    15901 tagcttactg acacagctgc tactggcaaa agatgttccc ctgccctttt tgctgaccgg
    15961 attaacgctg gtacttggcg tcactgctga gttggggcca ttgcacgcaa aattgcttcc
    16021 cgcatcgctg ctggcagcca tttttaccct cagtctggcg ggatacatgc cggtctggga
    16081 accgttgctc atctatgcgt tgggcactct ctggtacgga ttgtttaact ggttttggtt
    16141 ctggatctgg cgcgaacaac cgctgcgcga gtcactaagt ctgctgtacc gtgaactggc
    16201 agattattgt gaagccaaat acagcctgct tacccagcac accgaccctg aaaaagcgct
    16261 gccgccgctg ctggtgcgcc agcaaaaagc ggtcgatcta attacccagt gctatcagca
    16321 aatgcatatg ctttccgcgc aaaataatac tgactacaag cggatgctgc gtattttcca
    16381 ggaggcgctg gatttacagg aacatatttc ggtcagtttg catcagccgg aagaggtgca
    16441 aaagctggtc gagcgtagcc atgcggaaga agttatccgc tggaatgcgc aaaccgtcgc
    16501 cgctcgcctg cgcgtgctgg ctgatgacat tctttaccat cgcctgccaa cgcgttttac
    16561 gatggaaaag caaattggcg cactggaaaa aatcgcccgc cagcatccgg ataatccggt
    16621 tgggcaattc tgctactggc atttcagccg catcgcccgc gtgctgcgca cccaaaaacc
    16681 gctctatgcc cgtgacttac tggccgataa acagcggcga atgccattac ttccggcgct
    16741 gaaaagttat ctgtcactaa agtctccggc gctacgcaat gccggacgac tcagtgtgat
    16801 gttaagcgtt gccagcctga tgggcaccgc gctgcatctg ccgaagtcgt actggatcct
    16861 gatgacggta ttgctggtga cacaaaatgg ctatggcgca acccgtctga ggattgtgaa
    16921 tcgctccgtg ggaaccgtgg tcgggttaat cattgcgggc gtggcgctgc actttaaaat
    16981 tcccgaaggt tacaccctga cgttgatgct gattaccacc ctcgccagct acctgatatt
    17041 gcgcaaaaac tacggctggg cgacggtcgg ttttactatt accgcagtgt ataccctgca
    17101 actattgtgg ttgaacggcg agcaatacat ccttccgcgt cttatcgata ccattattgg
    17161 ttgtttaatt gctttcggcg gtactgtctg gctgtggccg cagtggcaga gcgggttatt
    17221 gcgtaaaaac gcccatgacg ctttagaagc ctatcaggaa gcgattcgct tgattcttag
    17281 cgaggatccg caacctacgc cactggcctg gcagcgaatg cgggtaaatc aggcacataa
    17341 cactctgtat aactcattga atcaggcgat gcaggaaccg gcgtttaaca gccattatct
    17401 ggcagatatg aaactgtggg taacgcacag ccagtttatt gttgagcata ttaatgccat
    17461 gaccacgctg gcgcgggaac accgggcatt gccacctgaa ctggcacaag agtatttaca
    17521 gtcttgtgaa atcgccattc agcgttgtca gcagcgactg gagtatgacg aaccgggtag
    17581 ttctggcgat gccaatatca tggatgcgcc ggagatgcag ccgcacgaag gcgcggcagg
    17641 tacgctggag cagcatttac agcgggttat tggtcatctg aacaccatgc acaccatttc
    17701 gtcgatggca tggcgtcagc gaccgcatca cgggatttgg ctgagtcgca agttgcggga
    17761 ttcgaaggcg taatgcaggc taaaccatcg gataaggcat tcacgccgca tccgacatct
    17821 tttgcctgat gcttcgtatt tacgccccaa ccaccttcgc caccgcgtgg gcgaaacgtt
    17881 gcatcccttc atcgatatcc gcatcttcca ccaccagcga cggtgcaaaa cgcatcacat
    17941 ccggtccggc attcagcacc attacgccag cccctgcgcc cgcatacagg aaatcacgcg
    18001 cccgaccttt gaactgtggc ttaagctccg cgccaatcaa cagccccata ccgcgaatat
    18061 cgctaaacac atcgtactgc tgatcgatct tctgcagatg gtcaacaaaa cgctggcgtt
    18121 tcgcctgaat gccttccagc acttcagggg tattgatgat atcaaacgcc gcgcccgcta
    18181 ctgcacagga cagaggatta ccgccgtagg tggaaccgtg agaaccagga tgaaatgcag
    18241 aagcaatttc tgccgtggtc agcatggcgc taatcgggaa gccgccgcct aacgctttcg
    18301 cagaggtcag aatatccggc gtcacgccgt agtgcatgta agcaaacaaa tcgccggtcc
    18361 gccccatccc gcactgtact tcatcaaaca ccaataatgc ctgatgttga tcgcacagct
    18421 cgcgcaagcc ctgcaaaaac tctggcgtcg ctgccgtcac accgccctcg ccctggatcg
    18481 gctcaaccac caccgcacag gtgtgatcat ccatcaccgc tttcactgcg tggagatcgt
    18541 taaaaggaac gtggatgatg tctgccggtt ttggcccaaa gccgtcggaa tattttggct
    18601 gcccgcccac cgagacggta aacagcgagc gaccatgaaa agcgttatgg aaggcaataa
    18661 ttttggtttt gaacggacta tgacgcacac aggcgtaatg gcgtgccagt ttaaaggcgg
    18721 tttcgttagc ttccgtgccg gagttcataa acaccacgcg ttcggcaaac gtcgcctcaa
    18781 tcagtttgcg cccaagacgc agcgccggtt cattggtgaa aacgttactg atatgccaca
    18841 gagtttcgcc ctgggttttt aacgcgttca ccagcgcagg atggcaatgg cccaacgccg
    18901 taactgcaat gccacccgcg aaatcgacat actccttgcc ttgctgatcc cagattcggc
    18961 tgcccagacc ttttaccgga atgaactctg ccggagcata aatcggcagg atcacttcat
    19021 cgaaagtcgc gcgtgtaatt gctgtttgtt caattgccat ctcatgatca ccctgttacg
    19081 cataaataaa tgtgaaatta taatcacaaa atatgcataa aaaatcattg aatggcaatc
    19141 agaaatcagc gatgcaggaa attagccagc agttgatgtc cttgttcgct aagaatactt
    19201 tctggatgga actgcacacc ttccagatcc cactggcgat ggcgaatccc cataatctcg
    19261 cgggtttcgc tccaggccgt cacgtcaaag catgctggta atgagccagg ttccaccacc
    19321 agcgaatggt agcgtgtcac ggtaagtgga tttgccagcc cccgaaatac gccctcaccg
    19381 ttatgtgtaa tcggcgaggt tttgccgtgc atgacctttg cggcgcgcac aactttgccg
    19441 ccaaatgcct gcgccattgc ctgatgaccg aggcagacgc caagaatcgg caagcgcccg
    19501 gcatagtggc gaataacgtc aagggagatc ccggcttcat ctggcgtaca ggggccgggt
    19561 gatatgacaa ttttttgtgg tttaagggcg tcgatatccg ccagcgtcaa cgcatcgttg
    19621 cgcttaacca gcacatccgc ccccagttca caaaagtact ggtagaggtt ccaggtaaaa
    19681 gaatcgtagt tatctataag caggatcatg gcggctccgg tacgaaagaa ccgcgctatt
    19741 ttactcagat tccccggctt cgcttaccac tttgctaaat atcatctgca gcgcggtgag
    19801 atcccccatt gccccactct gatttgcctg attccaggct tctttttcga taccctgcca
    19861 gctcagttga tatccggcat gaatcgccag ttgctcgaag aagatccgct gtgccagtcc
    19921 gcttcccaca cggaagggat gcagcacgtt gatttcacaa tagtaatgcg ccagccgctc
    19981 gacgaacttc gctttctcca ggccaaccag ataaccttct tcctccagat cctgcatcag
    20041 ggcattgccc tctttttcga tataagcaaa gtggcagaac ggcgtatcgc cctgataaat
    20101 atcaacttca cgcagttgcc ccgcccagtc gaaaatatcc tgatacagct ggcgatggat
    20161 agtgcgcaaa tgcggtaaac cgcgcaccag cggaccaagc tcaatggtcg cagcacgcag
    20221 cgccgtcatt tcgtaagcgg cctgttccag ccgctgctgc tggtggatgt tcagccggtt
    20281 acgcatgata tcaaggccag gataaagata cggatcgcgc ccttcgccga atttatcgct
    20341 catagtgcct cctcagctct tcaaggcgcg ctaaagcctc tgcggcagta agagtgacta
    20401 aaggcatctc gacgccttca agacggcgac tggcctggaa attacgatta cgctgaagct
    20461 cccagagacg ggacttttgc ttatcggtga gtttcttcac ttgatgcctc cctgaacgtg
    20521 tccatttgcc acaagtataa gcggcaaatc cgggttacgc cgggagagca gaagcgcggg
    20581 caagattgcc cgcgcgaaaa atcattacgg caggacttta gcggaaagga taactaccgg
    20641 ttttgacggc acattctggt acggaccaac gtcatgagtc ggcacctggg aaatcttatc
    20701 ggcaacgtcc atgcctttca ccactttacc aaataccgcg taaccgaaat cacgctgacc
    20761 atggtcaagg aaggcgttat cggcaacgtt gataaagaac tggctggtgg cgctgtcttt
    20821 gtcagcggta cgcgccatcg cgatggtgcc acgcgtgttg cgcaggccgt tatcggcttc
    20881 atttttgatt ggcgggtttg gttttttctg ctgcatctgc tcggtgaaac cgccgccctg
    20941 aatcataaag ccaggaatga cgcggtgaaa ggtagtgttg ttataaaaac cgctgttcac
    21001 ataatcgaca aagttttgca cagacactgg cgctttttgt ttatccagct ccagttcgat
    21061 gttaccagct gaggttgtca acaatacgtg cgggtcccct ttcgctgcca ttgctgcggg
    21121 agaaagagca gaaagagcga aaacagcagc catcgccgcc agggtcgatt tgaacatgag
    21181 atttccttaa caaacagagc agaaaaaaag caagtgaaga gattctaaat agccttcaag
    21241 aacaaggcca tccctttacc taattttacg tatctgaaat atatgtaacc tgacgatcac
    21301 tttttattcc gtgatcaaaa tcacctctta aaatgcaatt tagcaaccga ttgcaataaa
    21361 acatttaaac agatcacaaa atcacctaaa atcgcccgct cgcggcatat cccgctatgc
    21421 cattttattt ttgaacgcag gccaatcatg actaacagca atcgcatcaa gctcacatgg
    21481 attagctttc tctcctacgc actgactggt gcgttggtta ttgtcaccgg gatggtgatg
    21541 ggaaatatcg ccgattattt caatctgcct gtttccagta tgagtaacac cttcaccttc
    21601 ctcaacgccg gcattttaat ctctatcttc ctcaacgcct ggctgatgga aatcgtcccg
    21661 ttgaaaacgc agttacgttt tggctttctc ctgatggtgc tggcggttgc cggtttgatg
    21721 ttcagccaca gcctggcgct gttctcggcg gcgatgttca ttctcggggt ggtcagcggc
    21781 atcaccatgt cgattggtac attcctggta acacaaatgt atgaagggcg tcagcgcggt
    21841 tcccgcctgt tatttaccga ctccttcttc agtatggctg ggatgatttt cccaatgatc
    21901 gccgcgtttc tactggcgcg cagcattgag tggtactggg tttatgcctg catcgggctg
    21961 gtgtatatcg ctatttttat tctgaccttc ggctgtgagt tcccggcgct gggcaaacat
    22021 gcgccaaaaa cggatgctcc ggtagagaaa gaaaagtggg ggatcggcgt actgtttctc
    22081 tctgttgcgg cgctgtgcta catcctcggt cagttaggtt ttatctcctg ggtgcctgag
    22141 tatgccaaag gcctgggcat gagcctgaac gacgcgggca cgctggtgag taacttctgg
    22201 atgtcataca tggtcggcat gtgggcgttc agctttattc ttcgcttctt tgatttgcaa
    22261 cgcattctga ccgtactggc tggtctggct gcgattctga tgtacgtctt taacaccgga
    22321 acaccagcac atatggcgtg gtcaattctc gctctgggct tcttctccag cgcgatctat
    22381 accaccatca tcaccctggg ttcacagcag accaaagtac cgtcgccaaa actggttaac
    22441 tttgtcctga cctgcggaac catcggtact atgttgacct ttgtggttac cggcccgatt
    22501 gttgaacata gcggtccgca ggcggcactg cttacagcaa acggtctgta cgctgtcgtc
    22561 tttgtgatgt gcttcctgtt aggtttcgtc agccgtcacc gtcagcataa caccctgacc
    22621 tctcattaat tgctaatgcc ggacggcact atcgtcgtcc ggccttttcc tctcttcccc
    22681 cgctacgtgc atctatttct ataaacccgt tcattttgtc tattttttgc acaaacatga
    22741 aatatcagac aattccgtga cttaagaaaa tttatacaaa tcagcaatat accccttaag
    22801 gagtatataa aggtgaattt gatttacatc aataagcggg gttgctgaat cgttaaggta
    22861 ggcggtaata gaaaagaaat cgaggcaaaa atgagcaaag tcagactcgc aattatcggt
    22921 aacggtatgg tcggccatcg ctttatcgaa gatcttcttg ataaatctga tgcggccaac
    22981 tttgatatta ccgttttctg tgaagaaccg cgcatcgctt atgaccgcgt acacctctcg
    23041 tcttacttct ctcaccacac cgccgaagag ctgtcgctgg tgcgcgaagg gttctacgag
    23101 aaacacggca tcaaagttct ggtcggcgaa cgcgctatca ccatcaaccg tcaggagaag
    23161 gtgattcact ccagcgccgg acgtaccgtt ttttatgaca agctgatcat ggcaaccggt
    23221 tcctacccgt ggatcccgcc aatcaaaggt tctgatactc aggactgctt tgtctatcgc
    23281 accattgaag acctcaacgc cattgaatcc tgcgcccgtc gcagcaagcg cggtgccgtt
    23341 gttggtggcg gcctgttagg tctggaagcc gctggcgcgc tgaaaaactt aggtattgaa
    23401 acccacgtta tcgaatttgc ccctatgctg atggcggaac agcttgatca gatgggtggc
    23461 gagcagctgc gtcgcaaaat cgaaagtatg ggcgtgcgcg ttcacaccag caaaaacacc
    23521 cttgagattg tgcaggaagg tgtcgaagcg cgtaaaacca tgcgttttgc cgacggcagc
    23581 gaactggaag tcgactttat cgtcttctct accggtatcc gtccgcgcga taagctggca
    23641 acccagtgtg gtctggacgt tgctccgcgt gggggtattg tcattaatga ttcctgccag
    23701 acttccgatc cggatatcta cgccatcggt gaatgcgcaa gctggaacaa ccgtgtattt
    23761 ggtctggtag caccaggcta caaaatggcg caggtcgccg ttgaccatat tctcggtagc
    23821 gaaaacgcct ttgaaggtgc tgaccttagc gccaagctga aactgctggg cgtagacgta
    23881 ggcggtattg gtgatgcgca cggtcgcacg cctggcgcac gtagctacgt ttacctcgac
    23941 gaaagtaaag agatctacaa acgcctgatt gtcagcgaag acaacaaaac cctgctcggt
    24001 gcggtactgg tgggcgatac cagcgactac ggtaacctgc tgcaactggt gctgaacgct
    24061 atcgaactgc cggaaaaccc ggattccctg atcctgccag cacactctgg tagcggcaag
    24121 ccgtctatcg gtgttgataa actgccggac agcgcgcaaa tctgctcctg cttcgacgtc
    24181 accaaaggtg atctgattgc tgccatcaac aaaggctgcc acaccgttgc ggcgctgaaa
    24241 gctgaaacca aagcgggtac tggctgcggt ggctgtatcc cgctggtcac tcaggtactg
    24301 aacgcggaac tggcgaaaca gggcatcgaa gttaacaaca acctgtgcga acactttgct
    24361 tattcgcgtc aggaactgtt ccatttgatc cgcgttgaag gcattaaaac cttcgaagaa
    24421 ctgctggcga aacacggcaa aggctacggt tgtgaagttt gtaaaccaac cgtcggttcg
    24481 ctgctggcct cctgctggaa cgaatacatt ctgaagccgg aacatactcc gctgcaggat
    24541 tctaacgaca acttcctcgc taacatccag aaagacggaa cctactcggt gatcccgcgc
    24601 tctccgggcg gtgaaatcac cccggaagga ctgatggcag tgggtcgtat cgcgcgtgaa
    24661 tttaatctct acaccaaaat caccggctct cagcgcctgg cgatgtttgg cgcacagaaa
    24721 gacgatctgc cggagatctg gcgtcagctg attgaagctg gcttcgaaac cggtcatgcc
    24781 tatgcgaaag cactacgtat ggcgaaaacc tgcgtgggta gcacctggtg ccgctacggc
    24841 gttggcgaca gcgtcggcct cggcgtggaa ctggaaaacc gctacaaagg catccgtacg
    24901 ccgcacaaaa tgaagttcgg tgtctccggc tgtacccgtg aatgttcaga agctcagggt
    24961 aaagacgtgg gtattatcgc cactgaaaaa ggctggaacc tgtatgtttg cggtaacggc
    25021 ggcatgaaac cgcgtcatgc ggatctgctg gcagcggata tcgatcgcga aacgctgatc
    25081 aaatatctcg accgcttcat gatgttctac atccgtaccg ccgacaaact gacgcgtacc
    25141 gcgccgtggt tagaaaacct cgaaggcggc atcgattatc tgaaagcggt gatcattgac
    25201 gacaaactgg ggctgaacgc acatctggaa gaagagatgg cgcgcctgcg tgaagcggta
    25261 gtgtgtgagt ggactgaaac ggtcaataca ccgtccgcgc agactcgctt caaacacttc
    25321 atcaacagcg acaagcgtga cccgaacgta cagatggtgc cagagcgcga acagcaccgt
    25381 ccggcaacgc cgtatgaacg tatcccggta actctggtgg aggacaacgc atgagccagt
    25441 ggaaagacat ctgcaaaatc gatgacatcc tgcctgaaac cggcgtctgc gcgctgttag
    25501 gtgacgagca ggtagcaatt ttccgcccgt atcacagcga tcaggtgttt gcgatcagca
    25561 acatcgaccc gttcttcgag tccagcgtgc tgtcacgcgg gctgattgcg gaacaccagg
    25621 gcgagctgtg ggtcgccagc ccgctgaaaa aacagcgttt tcgcttaagt gatggcttgt
    25681 gcatggaaga cgaacagttt tccgtcaaac attacgaagc acgagtgaaa gacggcgtgg
    25741 tgcagctgcg cggttaatgt tttaacggga ggcgcaatgc ctcccctttt tgcatggtcc
    25801 tgtaatgacc ttcggtatat tgcaggacat tttttaaact ttttgtttta tttttttgtt
    25861 tttatttttt aaaggataat caaatgttta cagacactat taataagtgt gcggctaacg
    25921 ctgcgcgcat tgcacgcctg tcggcaaata acccgctcgg cttttgggtc agctccgcca
    25981 tggcgggcgc gtatgtgggt cttgggatca tcttgatttt cacgctcggt aatttgctcg
    26041 acccgtccgt acgtccgctg gtgatgggcg cgacctttgg tatcgcctta acgctggtga
    26101 ttatcgccgg ttctgaactg ttcaccggac acaccatgtt cctcaccttt ggggtaaaag
    26161 cgggcagcat cagccacggg caaatgtggg caatcctgcc gcaaacctgg ctgggtaacc
    26221 tggtcggttc cgtcttcgtt gccatgctct atagctgggg cggcggtagc ctgctgccgg
    26281 tagataccag catcgttcac tccgtcgcgc tggctaaaac cactgcaccg gcaatggtac
    26341 tgttcttcaa aggtgcattg tgtaactggc tggtttgcct ggcaatctgg atggcgctgc
    26401 gcactgaagg ggcggcgaaa tttatcgcta tctggtggtg tctgctggca tttatcgcgt
    26461 ctggctacga gcactctatc gctaacatga cgctgttcgc gctctcctgg ttcggcaacc
    26521 acagcgaagc ctacacgctg gcgggtattg gtcataacct gctgtgggtg acgctgggta
    26581 atactttatc aggtgccgta ttcatgggat tgggttattg gtatgctacg ccgaaagcga
    26641 atcgtccggt tgcggacaaa tttaatcaaa ctgaaacggc tgccggttaa ttactaaggg
    26701 gtttttacgt ggatcatttg cctatatttt gccaattacg cgatcgcgac tgtctgattg
    26761 tcggcggtgg tgatgtcgcg gaacgcaaag caaggttgct gttagacgca ggcgctcgct
    26821 taacggtgaa tgcattagcg tttattccac agttcaccgc atgggcagat gcaggcatgt
    26881 taaccctcgt cgaagggcca tttgatgaaa gccttctcga cacctgctgg ctggcgattg
    26941 cagcgacgga tgatgacgcg cttaaccagc gcgtcagcga agccgctgaa gctcgtcgca
    27001 tcttctgtaa cgtggtcgat gcgccgaaag ccgccagctt tattatgccg tcgattattg
    27061 accgctcacc gctcatggtc gcggtctcct ctggcggcac ctctccggtt ctggcgcgcc
    27121 tgttgcgcga aaaactcgaa tcactgctgc cgttgcatct gggccaggtg gcgaaatatg
    27181 ccgggcaatt acgcgggcga gtgaaacaac agttcgccac gatgagtgag cgtcgccgtt
    27241 tctgggagaa attgttcgtt aacgaccgcc tggcgcagtc gctggcaaac aacgatcaga
    27301 aagccattac tgaaacgacc gaacagttaa tcaacgaacc gctcgaccat cgcggtgaag
    27361 tggtgctggt tggtgcaggt ccgggcgatg ccgggctgct gacgctgaaa ggactgcaac
    27421 aaattcagca ggcagatgtg gtggtctacg accgtctggt ttctgacgat attatgaatc
    27481 tggtacgccg cgatgctgat cgcgttttcg tcggcaaacg cgcgggatac cactgtgtac
    27541 cgcaggaaga gattaaccag atcctgctgc gggaagcgca aaaaggcaaa cgcgtggtgc
    27601 ggctgaaagg tggcgatccg tttatttttg gccgtggtgg cgaagagctg gaaacactgt
    27661 gcaacgcggg tattccgttc tcggtggttc cgggtattac cgcagcttct ggttgctctg
    27721 cctattcggg tattccgctc acgcatcgcg attatgccca gagcgtgcgc ttaattaccg
    27781 gacacttaaa aaccggtggc gagctggact gggaaaacct ggcggcagaa aaacagacgc
    27841 tggtgttcta tatggggctg aatcaggctg cgactattca gcaaaagctg attgaacacg
    27901 gtatgcctgg cgaaatgccg gtggcaattg tcgaaaacgg aacagcagtc acgcagcgcg
    27961 tgattgacgg tacgctcacg caactcggtg aacttgctca gcaaatgaac agtccatcgc
    28021 taattattat tggtcgggtt gttggcctgc gcgataaact gaactggttc tccaaccatt
    28081 aatttaaccc gggccagaga attctggcct tcttaatgac tctttttata tattcagcaa
    28141 ataaaacata gccccttaat aaatattatc tgctaatggg ttatattcac cgttacagcg
    28201 acttacaaaa ttaagtcatt cgctcactga cgccaattaa gtattcctgt atcgttctta
    28261 acgagcagtc tgcttatatt catataatca atgaatatta attaatatat aatacatagg
    28321 gaatgtaaat gaacaaattt attaaagttg cactggtagg tgcagtactg gctacgttaa
    28381 ctgcatgtac tggtcatatt gaaaaccgtg ataagaactg ctcttacgac tacctgctgc
    28441 acccggcaat ttctatttct aaaatcattg gcggttgcgg tcctactgca cagtaagtct
    28501 ctgacagaaa tcggctaaca ccggtttaat tgcccgacgc cctctggatt ttccgagggc
    28561 gtatttgttt ctatatcctt cttgctcccc acccgtaaaa atattttcac tttaatttca
    28621 atttgataac cacattcatt agccacagcg cgatcctatg cgaaattttg tgatcttcct
    28681 ccgcattaca taacatcata tgttgttata ttcattatgc attgtcatgt taccttttaa
    28741 atgactgcaa actctcccct acaacgtgtt ggacaagaaa aaggtatcgc tatgggaagc
    28801 caggaactcc aacgcaagct cggattttgg gccgttcttg caatcgccgt cgggacaacc
    28861 gtcggctccg gtatttttgt atctgtgggt gaagtggcaa aagcggcggg cacgccgtgg
    28921 ctgacggtgc tcgcatttgt catcggcggg ttaattgtga tcccgcaaat gtgcgtctat
    28981 gcggaactat caaccgctta tccggaaaat ggcgcagatt atgtttatct gaaaaatgcc
    29041 ggaagccgac cgctggcttt cctctccggc tgggccagct tctgggccaa cgatgcgccg
    29101 tcattgtcga ttatggcgct ggcgattgtc agcaatcttg gctttttaac gcctatcgat
    29161 ccgttgcccg gtaaatttat cgccgccgga ttaattatcg cctttatgtt gctacacctg
    29221 cgctccgttg aaggcggcgc agcgtttcag acgctaatta ccatcgccaa aattatcccg
    29281 ttcactatcg tcattggcct tgggatcttc tggtttaaag cggagaattt tgccgcccct
    29341 accaccactg cgattggcgc aacgggcagc tttatggcgc tgctggcggg gatctctgcc
    29401 accagttggt cgtataccgg catggcctct atctgttata tgaccggcga aattaaaaac
    29461 cccggaaaaa ccatgccacg agcgctgatt ggttcctgtc tgctggttct ggtgctctac
    29521 accctgctgg cgctggtgat ttccggcctg atgcccttcg acaaactcgc caattctgaa
    29581 acgccgattt ccgacgccct gacctggatc cccgcactcg gcagcaccgc tgggatcttt
    29641 gttgccatca cggcgatgat cgtcattctt ggttcgcttt ccagctgcgt gatgtaccag
    29701 ccgcggctgg aatacgcgat ggcgaaagac aacctgttct ttaaatgctt cggccatgtg
    29761 catccgaaat acaacacgcc ggatgtctcc atcatcctgc aaggggcgct ggggatcttc
    29821 ttcatcttcg tttccgatct caccagcctg ctgggttatt tcaccctggt gatgtgtttc
    29881 aaaaataccc tcaccttcgg ctccatcatc tggtgtcgta aacgcgacga ttacaaaccg
    29941 ctgtggcgta ctccggcttt cgggctgatg accaccctcg ccattgcgtc aagcctcatt
    30001 ctggtcgcct caacctttgt ctgggcaccg attcccggcc ttatctgcgc cgtcatcgtt
    30061 attgctactg gtctgcctgc ttacgccttc tgggcgaagc gtagccgcca gctcaacgct
    30121 ttgtcgtaat gttatctgga gaaaataaaa tgttggatat tgataaaagc accgtggact
    30181 ttctggtcac cgaaaatatg gttcaggaag tggaaaaagt tctcagccat gacgttccgc
    30241 tggtgcacgc catcgtggaa gagatggtga agcgcgacat tgatcgtatt tatttcgttg
    30301 cctgcggctc gccactcaac gcggcgcaaa cggcgaaaca tctggcggat cgcttttccg
    30361 atcttcaggt ctacgccatt tccggctggg agttctgcga taacaccccg tatcgcctcg
    30421 acgatcgttg cgcggtgatt ggcgtttctg actacggtaa aacggaagag gtaatcaaag
    30481 cgctggagct gggccgggcc tgcggcgcac tcactgcggc gttcaccaaa cgcgcggaca
    30541 gcccgattac ctcggcggcg gaatttagca ttgattatca ggccgactgt atctgggaaa
    30601 ttcacctgct gctctgctac agcgtggtgc tggagatgat cacccgcctc gcgccgaacg
    30661 cggaaatcgg caagatcaaa aacgatctca agcagttgcc gaatgcgctc ggtcatctgg
    30721 tacgcacctg ggaagaaaaa ggccgccagc ttggtgaact ggccagccag tggccgatga
    30781 tttataccgt tgctgcgggt ccgctgcgtc cgctgggtta caaagaaggc attgtaacgc
    30841 tgatggaatt tacctggacg cacggctgcg tgattgagag cggagagttc cgccatggcc
    30901 cgctggagat tgtcgaaccg ggcgttccgt tcctgttcct gctcggcaat gatgaaagtc
    30961 gccacaccac cgaacgcgcc attaactttg ttaaacagcg tactgacaac gtgatcgtca
    31021 tcgattacgc cgaaatttcg caagggctgc acccgtggct ggcaccgttc ctgatgttcg
    31081 tgccaatgga gtggctctgc tactacctgt ctatttacaa agatcacaac ccggatgaac
    31141 gccgctatta cggtggtctg gtggaatatt aatccctctc ccggcccggc aacgggccga
    31201 atttacgcaa ggagttaccc gatgaaaaca ggtatgttta cctgcggcca ccagcggctg
    31261 cctattgaac acgcatttcg tgatgcaagc gagctgggtt acgacggcat cgaaatttgg
    31321 ggcggtcgcc cgcacgcgtt cgcgccggac ttaaaagcgg gcggcatcaa acaaatcaag
    31381 gcgctggcgc agacgtatca gatgccgatt atcggctata cgccagaaac caacggctat
    31441 ccgtataaca tgatgctggg cgatgaacat atgcgtcgcg aaagcctcga catgatcaag
    31501 ctggcgatgg atatggcaaa agagatgaac gcgggttata cgctgatttc cgcggcccac
    31561 gcgggctatc tcacgccacc taatgttatc tggggacggc tggcagagaa cctaagcgaa
    31621 ctgtgtgagt acgcggaaaa catcggcatg gatctgatcc tcgaaccgtt aacgccgtat
    31681 gaatcgaacg tcgtgtgtaa tgctaatgat gtgcttcatg cgctggcgct ggtgccttcg
    31741 ccgcgcctgt tcagcatggt cgacatttgc gcgccgtatg tccaggcgga accggtgatg
    31801 agttatttcg acaaactggg tgataaattg cgtcatctgc atattgtcga cagcgacggg
    31861 gccagtgaca cacattacat tcccggcgaa ggcaaaatgc cgctgcggga gctgatgcgc
    31921 gatattatcg accggggcta tgacggttac tgcacggtgg aactggtgac gatgtatatg
    31981 aacgagccca gactctatgc ctgccaggcg ctggaacgct ttcgcgcgct gctgccggag
    32041 gatgagagat gaaaaccctg gcgacaatcg gcgataactg cgtcgatatc tacccgcaac
    32101 tgaataaagc gttttctggc ggtaatgcgg tcaatgtggc ggtgtactgc actcgctacg
    32161 gcatacagcc gggatgcatt acctgggtgg gtgacgatga ctacggcaca aagctgaagc
    32221 aggatctcgc ccgcatgggc gtcgatatca gccatgtcca tacgaaacac ggcgttaccg
    32281 cacaaactca ggtggaactg cacgacaatg atcgcgtttt tggcgactac accgaaggcg
    32341 tgatggccga ctttgccctg agtgaagagg attacgcctg gctggcgcag tatgacattg
    32401 tgcacgcggc aatctgggga catgcggaag acgcattccc acagctgcac gctgcgggca
    32461 aacttaccgc tttcaacttc tccgacaagt gggacagccc gctctggcag acactggtgc
    32521 cgcatctcga ttttgccttt gcctccgcac cgcaagaaga cgaaacgctg cgtctgaaga
    32581 tgaaagcgat tgttgcccgt ggcgcaggaa cagtgattgt cacgctgggt gaaaacggca
    32641 gcattgcctg ggatggcgcg cagttctggc gtcaggctcc tgaaccggtg acggttatcg
    32701 acaccatggg tgccggagat tcgttcattg ccggattcct ttgcggctgg tctgcgggga
    32761 tgacattacc gcaggcgatg gcgcagggaa cggcgtgcgc ggcgaaaacc attcagtacc
    32821 acggtgcctg gtaggtataa cgttggcgtg agcatcttca cgccaacgtg ctgttacttg
    32881 ccggaaaacg accctataat ccgagtaatt cattctttat ttcagggtcg attatgtcag
    32941 ctacggaccg ctactctcat caactcctct acgctaccgt ccgccagcga ctgctggatg
    33001 atatcgcgca gggggtttac caggccgggc aacagatccc taccgaaaac gagctttgta
    33061 cacaatataa cgtcagccgc attaccattc gcaaagccat cagcgactta gtggcagacg
    33121 gcgtactgat ccgctggcag ggaaaaggca cctttgtaca aagccagaaa gttgaaaacg
    33181 ccctgcttac tgtcagtggt tttaccgatt ttggcgtctc acaaggcaag gcgacgaaag
    33241 agaaagtgat cgaacaggaa cgggtcagcg ccgcgccgtt ttgcgaaaag ctgaacatcc
    33301 ccggaaacag cgaagtgttc catctctgcc gggtgatgta tctcgataaa gagccgctgt
    33361 ttattgatag ttcatggatc ccgctgtcgc gttatcctga ctttgatgag atttacgtcg
    33421 aaggaagctc cacctatcag ttatttcagg agcgttttga cacgcgagtg gtcagcgaca
    33481 aaaagaccat cgatatcttt gccgccaccc gcccgcaggc aaaatggctg aaatgcgaac
    33541 tgggcgaacc gttgtttcgc atcagcaaaa tcgcctttga tcagaatgac aaaccggtgc
    33601 acgtctccga actcttctgc cgcgccaatc gcatcacctt aactattgat aataaaagac
    33661 attaaccgta ggccggataa gatgcgccag catcgcatcc ggcgatgctg gcgcgttgaa
    33721 ttttacatcc cgtacgttcc cctcacccta accctctccc caaaggggcg aggggaccgt
    33781 tcagtacgga atttagcttg acggtgctat ccagcattaa atactggcaa tactctcacg
    33841 caaaatccgc agcaccgtct cttcaccgct gcgattcggg ttaatgcgaa tcgcacaatg
    33901 ttctgattgt ggattcgcct ggcgaaacgt tccggaaagg cgataaaaga gcggcgggat
    33961 ttcatatttc gactctgcac ccaccgggta aggcaaggca ccgcgctttt gcgcctcttc
    34021 cagcactctg gcggcaatcg gctgatgaaa ctcgacaatc aacaccttcg actgcgcatt
    34081 agcaatcacc gcgcttttca cttccggcac cgcaccaccg ttaagcaaag ccagcaaccg
    34141 ttcagatacc cctgcctgca ccgcgtgcat cactggcgca aacaccagac cacgcaatac
    34201 ttccagcgcc tgtgcgccct ggatctggct accgccggag taaagcgtgg cgcgaatacg
    34261 gttgataaca tcagcatcgc cgaccactgc accaacaccc tctggcccaa atagcttgaa
    34321 gcaggaaaat gtcgagacat tcgcgccgca ttcacagccg attcgcgcca ccttcatcac
    34381 cgcatagttg tcatcggtta acgctggaac acctgccgcg cgcaacgttg ccagcacatc
    34441 tgccagcacg tagctgtcct gcggctgctg gcgcgtatgc tgcaccagcg ccgcatccgg
    34501 ttgttgctcg tcgacgacct gcttcagtgc cgacaggtca ttgaaatcaa cagtaataag
    34561 cgtcagcccc atctgctcaa taataacccg tgtcgtcggg taaacaggcg cgtcatgcac
    34621 cagaagacgc tgccccggtt tgagcaaagc cgccagcccg gcgcgaatcg cgccagtccc
    34681 cgcgccctgc accagcgccg cagcctgtgc gtgaaatgca tcagccagca cctgttccac
    34741 acgctgggta acgcgcggtt gattcagtcc tggcgttaag cctaaatcac cgccagtaag
    34801 aaactcgctg ccggggaaat ggcgacaaat gctatccacc agcgcaaact gcttttgctg
    34861 cgcctcaata atcgtcaggc tttgcagagg aaacgtcttc ataggtgcct tccttaagcc
    34921 ggaacgaaca ggccaagcca gtaaagaaca ttcagcagaa taccggtgat cattactgcc
    34981 acgaccggtg ccgccatttt ctgtaccgga cggcccaggg attcgttgag gaagtaaatg
    35041 gcaaccgcga tagagaatcc ggtataaccc gccatcttaa ttgctgcgaa aatcgaaccg
    35101 accagcagcg ccacttccat cagcatattc atggcgttac ggatgttatc cgacgcatta
    35161 cgcaccgacg gatagcgccc cagccatttg ccgatcgaac gaagcaataa aacttctgcc
    35221 gaaataacca ctgcacctaa taccgctgca accatcggat tcggcgagag atagcccacc
    35281 gcataaacaa aggtaaagcc cgcaactgca tacacacccg ttgctaacgc ggtggtggca
    35341 atcaacggta caaaacccag cccgcgcata aattctgcca gtgccgcctg gttgatcagc
    35401 gtttgcgatt gttccggcgt tacgcctgcg gagtacgctt tctccagagt gaagatcgac
    35461 acttcactgc cggcgaaaat cttcatgctg gcaacggcgg caatcaatgc tcccacgatg
    35521 gcgatatagg gtaagttttt gataatccgc gacgtgcgtt cttcaaacac cgaaagcccg
    35581 ctggcatcaa tatcattttc atcacgatga cgcaggtcgt gagttatcgc gatccccagc
    35641 agcatcacca tgccaataaa gatttcgatg gattcagggt taagatgtgg gaaatagcgc
    35701 acgactacca cacgggtcat cagaacaacc acggcggcga ccagactttg cttccagcca
    35761 aactggtaga aaatcgccac cagcgggaac agtgcaaaag cagaaaccac cggcgagctt
    35821 aattccccga ggctacctaa tacatccacc ggcagcgcgg tcagcagctg gtttactggc
    35881 aacaggcaag taaggatcaa cacgccccag atagcgccaa gaccaaacgc catcaggctg
    35941 tttatcgcca gtacgccgag gatatcggta ggaagaaaga gtaaccatgc gttgagcagc
    36001 ccggttttca gggtaaaaga gatccccacc gaggccacaa aaccaatgct cagaccaaaa
    36061 gcgatactcc ccgcctcgcg acgattcata tagccttcaa tcagttgcgg caggatcggg
    36121 cggatgccgt catgaaaaac agccgccgag cgatgcgcca gaagcgatgt catacccgtc
    36181 aggcacgcca ccacgataat ctgaatatac agatccatag tcgtgccttt tatttcagat
    36241 ggttaatcag catcggaatc gcgtgttcga cgtgctcaac ggaaaggcca aacgccactt
    36301 tcccttcagc gatcattttg gcgatatgct cgtctttcgc tttaatgcct ggtttggcaa
    36361 tggtgcaact tttgttatag ccaatcaccg cgatggcaat cgacaatgcc gcgcccgcac
    36421 cggtattaca cgcaccgatg tagtaatcca gttgcccgga tttcactttc attgccgctt
    36481 ccatgtcgtt gtgaatgaaa acttcaaaac tgccaggagc cgtcgcttca atggtttttt
    36541 taatctgctc acgctgtaag cctgcaacgc caatcttttt catgagtctg tccttattgg
    36601 aaaaattgag agggattttc acgtaacatc acatccacat cggcctgact gaatcctgac
    36661 tggcgcaatt gcggaataaa ggtggtcaat aaatagtcat agccataacc accattggct
    36721 tttaaatggg agcggcgcgt aatatccatc gacagcatga cgcggttcag caacccacgg
    36781 tcacgtagcg cgtggagcat cgcaatgcgc ttttcgtccg ggtagtaact gttcttacca
    36841 atggtgtcga actgcacgta cgcgccgaga tcgatcatct tcaaaatgtt gtcgagattg
    36901 tctttcagat cgcagtgacc aacggtgacg cgcgaaagat caactccgtg ggcttgcaac
    36961 agagccagtt gttccagccc catggtgctg aacgaagtat gcgtggagat cgggcgtccg
    37021 gtctggttat gcgccagcgc agcggcaata aacaccttct cttccagcgg cgtaatcttc
    37081 ccttcgctag agccgatctc cgcgataatc ccggctttca ggtcggtgcc atcgataccc
    37141 tgttcaattt catcgaccat ctcctgcgcc agttcctgca cgctgcgggt cgccacatgt
    37201 tccgggaaaa acgcgtcctg gtaataaccg gtacaggcca ccacgttgat ccccgtctcg
    37261 cgcattacat caagcataaa ttgcgcattg cgccccatgt aacggttggt catctcaatc
    37321 acattacgca cgccccgggt catcaggtcg ttcatctcct ggcaaatgaa cgcatactga
    37381 tcaaggcggc agtccacgtt gtttttaaag ccggagagat caatatgcag atgctcatgg
    37441 gcgagggtgt aacccgtcgg atcaaaactc atagggtgtc tcctgcaaac cggagggagg
    37501 aaagaaaaga gcgaccattt tgtggcggtg gcgcgcggaa aaattcacac acggtagccc
    37561 ccacatcaga aagcgtggtg cgcacaccga gctgcgtagc gatcatccct tgctgataaa
    37621 ccagcactgg caccacttcg tgggtatggt ggctgtgacc aatggtcgga tcgttaccgt
    37681 gatccgccat cacgaccagg caatcatctg gctgcatcgc ctcaacaagc cgggcaaggt
    37741 tacggtcaac gacctgcaaa cgttcggcat aacgtgcgac gtcttctgca tgaccagcga
    37801 ggtcggtttc ctggatattg atgcaaataa acgccgtcgg atgcgtgtta aattcgtcga
    37861 gggtgatatc cataatccgc tggctatcca ccagattttg ccagctcacg ccataaggat
    37921 tgttgacgat atctgccacc ttacccacca gcacggttgg cacgcctgct tcatacagtt
    37981 tttgtggcac ctgcactttt tcatcgacgc catagcccat atgcacgacc tggaaaccgt
    38041 tgtcataagc gccagaacgc ggcgcattga taccaataaa gcgcccttct ttgctttctg
    38101 cggcatcgag aatgcgttga ctgtcggtta acaggccacc aaatgtaatg acccgaccga
    38161 cctgtacctg ctcacgcacg atacgaccaa ttttgattgc gtcgtcaaaa gagatcacag
    38221 agagattggc ggtaatgtta tagacctggc ctaaatccgc ctcgagatta tcgccaatcg
    38281 caaccgcctg attgacccac agaaattgca gttcatcgcc acggcgctcc acctgccaac
    38341 cggcggatac taatgcctgc tcaacacggt caatcacatc gcaaaaaggc atccgcagcg
    38401 gcggtaacgg gcgcgtgcct aaaatttcct gatgccccat aaaggtatcg ccaccttcat
    38461 gttgcagctc tgccacgccc caggttgcgg aatctgacgg ctgcatatcg cctggcgcat
    38521 aacccaatgc gttgattagc cccagcttct ccagcgttgg tagctgcaaa tgcggcaact
    38581 ggctcaggat gtgaccacag gtattcgctc ccgcatcttg cggacgcacc agcgtgacat
    38641 ctttcattgc ccctacgcca aagctatcaa tcactaacac cacaaatcgc gccattacgc
    38701 ccccagagag tttccaagac tgtcataacg accaacgatt ttcggttcgc cacggtgaat
    38761 accggacacc agcaccacat cgctacgggt gacaaaaatc tgcgtgcgga aacagagcac
    38821 cactgcactg ctcaccggat actcgcctgc cagcggcagg gtgtagtcga tactgctgtc
    38881 atccacagtt ttgagattgg tttcagtaat cttttgattt tctggcgtaa acaccagcgc
    38941 atgttgcgca tgaccacgac ggtagtaacc gccgccgtag cagtagctgt cgccacggaa
    39001 atgatgggag atttcactta accagagcat cgcgatacgt tcaggctgat cgccctgctg
    39061 gtttgccgga atagtgcccg tcagcgcatg accgggttcg gcatgagtca caccgtattc
    39121 cgccagcaat ggcagcgaag tgcagctggt cgctgaaggc gcgttcagtt gctcaagtgc
    39181 aataccagat ttcgccagtt gatcccgtgc ctgtatcagc gtgtgaagat tcggtgtcgg
    39241 caaaactttt ccggcagcct catcccaaag caggcaaggg aaatgggtaa gtccggctaa
    39301 atgcagcccc ggcagtttct taatttcagc aaccacctcg tgcagcgaat gctgtacaaa
    39361 accgctctcc tgccccggat aaagaaaatc atcgtcgcta taaactttga gcagcacaaa
    39421 ctgcacacgt ccggccttca ccgctgccgc agaaacttcc cgcgctttgt cgagagtaaa
    39481 cacggtgatg acgtcggtgc cctgttcaac ggcgtcagca acctgatgac aagggatttg
    39541 taccagatgc ccctgatgcg ccaccgacaa accagcgcgg cgcatgactc gcgcctcttt
    39601 gtaatccacc gccacaatgc cgctgtaacc taatgccagc aatttttccg ccagccatgg
    39661 gttacggcca aactgcttgg tcatcagata cagttcaatg ccgtaaagcc gcgccgtctc
    39721 aatcagtcgc ttaccgtttt ccagtacctg atccacgtcg atcacccagc tgtccggggc
    39781 gatcttgccc tgctgccaca aacttagtgc ggcggaaatc agcgccgggt tctggcgttt
    39841 caatgcttct acaaacatct tgttgcctcg tttctgttcc ttgaattaaa tattcatttt
    39901 tttgaatatt taggttgtgc ggcgtggatt tgtcgggagc ttcaaacctc ttcgttagca
    39961 gccatccata atccataaag gttggccagc aaatagcctt cttcacacgg gttaacttcc
    40021 agtgcgaatt ccttcaacaa cacctgatgc aattgcacaa cggcttgcca gtggctggat
    40081 tgcgccagct ctgccagcaa ttcgttatcc agcggctcta tttcttcacc acggcgactg
    40141 cgcatcagtg cactcgccat atgtgtcatc gccatcgttc cttgctcact gcgcaccggc
    40201 agatggcact ctgtttccag tacgttgacg acctgcatca tgcctttgca gatgtcctta
    40261 tcaataacgc ctgcctcgca aagcaggttg agtctggttt ccatatcaga tgcctttcgt
    40321 ttaataactc ggttcctgtt ccccactcac tacccgttgt tgatgggcga gcagggcgtg
    40381 tttatctaca acggcacgca gcagttgttg cattgggtaa tcatcgactc gcgtcaggac
    40441 cacggcttcg gtggcctgta aaaatcgcgg atcgtctgtg agcggcgtcg cctctaatcc
    40501 cagcatggtc agttcgtttt ccgccaccac gttccagatc accgcatcga catcgccttt
    40561 aacaatgcgt tgtaaactct cgtgataaga gagatcgact cgttccacat cactaccgcc
    40621 aaaaaaaaca tcggtcatga ttttctgatc cgccgaacgg ttatccagcc ccacgcgctt
    40681 cacgtttgcg gactcgcctt tacggcaaat caactggtgc tcgccaacgt aggtgtgcgg
    40741 ccccaactcc agcgcgaggc ataacccttt ttgcgtgaga taactttccg ccgccagtcg
    40801 ggaaaccacc gccatgtcat acacgccgtt aagtaaacac tccacgcgaa tatccgcgcc
    40861 gcgcatatgc gcatagtaaa aaggaatgcc atcaaactga gctttcagcc cgctcgctaa
    40921 accttcgtac aagcgggtat agggcaaagg catcgcacat accacgttgt tgatatccac
    40981 atgactcagc aatgctttgt tatccatctc gaccagataa ctgccattgc gcccacggcg
    41041 ttcaatccgt atcgctccgc ttgattccag cgttttcaac gcggcctgcg tcaggccaac
    41101 cgatgaacgg cattcagttg ccagctcatc tatggttttc aacctattac cgcatttttc
    41161 acccaacaaa taacgggcca gcgtcgtgat aacgacgcct tcttttttga taaacgttcg
    41221 acgcataata tattttcaat aaaatgaatg tttatatctt cattaaaatg aagataagtg
    41281 gcaactgtga gatgtggaaa cggcgaggcg cttcacattt tttcggatta ttgcaaatga
    41341 ggataaaaaa accgggtttc cccggtttca gagtgatgac gaagtgattt attcgacaat
    41401 gcgagcaaag ggtaattccc actggcgctt gcctgctccc ctcaccctaa ccctctcccc
    41461 aaaggggcga ggggaccgtc cgagcgtttt gatattacgg cttcgccaca aaaccaatcg
    41521 cttcgtacac cgctttcagc gtacgggaag cgtgtacact ggctttttct gcgccgtctt
    41581 tcatcacctg ttgcaggaag gcttcatcgt tgcggaaacg gtgatagcgt tcctgcaatt
    41641 cagtcagcat accggaaacg gcatcagcca cttcaccttt cagatgacca tacatcttgc
    41701 cttcgaactg tttttccagt tctgggatgc tctggcccgt taccgcagaa aggatatcca
    41761 gcaggttgga aacgcccgct ttgttctgca catcgtagcg aactaccggc ggctcgtcgg
    41821 agtcagtgac cgcgcgtttg attttcttca ctaccgattt cggatcttcc agcaggccga
    41881 taacgttatt gcggttatcg tcagacttgg acatcttctt ggtcggctcc agcagcgaca
    41941 ttacgcgcgc gccagatttc ggaataaacg gctccggcac cttaaagatc tcgccataca
    42001 gcgcgttgaa acgctgggcg atatcgcgac tcagttccag gtgctgtttc tggtcttcac
    42061 ccaccggtac cagattagtt tgatacagca ggatgtccgc agccatcagc accggatagt
    42121 caaacagacc agcgttgatg ttctcggcat aacgcgcaga tttatcttta aactgggtca
    42181 tgcggctcag ttcgccgaag taggtatagc agttcagtgc ccagcctaac tgcgcatgtt
    42241 ctggtacgtg ggactgaaca aaaatggtgc ttttctcagg atcgatacca caagccagat
    42301 acaaggccag cgtatccagc gtcgctttac gcagcttctg tgcatcctgg cgcacggtga
    42361 tcgcgtgttg gtcaacgata cagtaaatgc aatggtagtc atcctgcatg tttacccact
    42421 gacgcagcgc acccatgtag ttaccaatgg tcaattcacc tgagggctgt gcgccactaa
    42481 aaacgatggg cttagtcatt tttcgattcc tgattttcgc tatgcggaag ccctaatgcg
    42541 ggcagaaggt catttataga ctgataaatt acatcaggct ggctgagatc gatagcctcg
    42601 ccgtagttat atccgtaggt taagccaacg gatgggcaac ctgccgcttt tgccgcctga
    42661 atatcattgc gtgagtcgcc gacaaacagc atctgttgtg gggcaattcc catccgctca
    42721 gccaccagta acagcgggtc cggatgaggt tttttgtttt gcacatcatc gccgccgata
    42781 accacgctaa agtatttggc aatatctaag gcttcgagca gcggcgcgac gaacggcgtc
    42841 ggtttgttgg tgaccaggcc tagcggcagg cctttagcct gcaacgcgcc caacgtatcg
    42901 gcaacgtgcg ggaacaaaaa cgtcccctct tcggcaacct cgccatagta gcgatcgaac
    42961 agtttacgca gaatacgtac ctgttcttct gccggaatgt cgtcatcaac gggcggttta
    43021 cccattgttt tacgctgagt cgcacgttcc tgacgcgccc aggtcaatgc gcgctccatc
    43081 agaacatctg cgccgttacc aatccaggta ataacgcgtt cttcacctgc gacgggcaac
    43141 tccagcgcat acagcgccat atctaccgca gcagcaagac cgggagcact gtcgaccagc
    43201 gtaccatcaa gatcaaaagc gacgccgcga atatcttcaa acttattcat gacttacctt
    43261 tgccagttca ctgcgcattt catcaatgac ttttttgtag tctggctggt cgaagattgc
    43321 cgaaccggcg acgaacatat ccgcgcccgc cgcagcgatt tcgccaatgt tgttcacctt
    43381 cacgccaccg tctacttcca gacgaatgtc aaagccagac tcgtcgatac gacgacgtac
    43441 ttcgcgcagt ttatccagtg tttgaggaat gaacgactga ccgccgaaac caggattgac
    43501 ggacatcagc aggatcacat ccagcttatc catcacgtaa tccagatagc tcagcggtgt
    43561 cgccgggtta aataccagtc ccgctttaca gccattttct ttaatcagtt gcagcgtgcg
    43621 gtcaacatgc tcggaggctt ctggatgaaa ggtaatgatg ctggcaccag cggcagcgaa
    43681 atcaggcaca atgcgatcga caggtttcac catcaggtgt acgtcgatag gggcggtaat
    43741 gccgtagtta cgcaaggatt tcagcaccat tggcccaatc gtcagattgg gaacatagtg
    43801 gttatccatg acgtcaaaat gcacgacatc agcgccagct gccagggctt ttgcggtatc
    43861 ttcacccagg cgggcaaaat cagccgacag aattgagggg gcaatcaaat actgtttcat
    43921 ccgcttctcc ttgagaatta ttttttcgcg ggtgaaacga ctcctggttt gtacaaagcc
    43981 agcagttcgt ccaccttttt acgtgtgccg ccgttgctgc ttatactgcg tcgaactttg
    44041 acgacatgca attttgcgcg ctgataccac tcacgcgtta acatcgtatc gtgattggag
    44101 atcagcactg gaatatggcg ctcaaccaga ccttcggcga tctccgccag atgcgcttgt
    44161 tgttcaagcg taaaactgtt tgtgtgatac gccgtaaagt tggcggtcgc agacagcggt
    44221 gcataaggcg gatcgcaata gacgacggat gcatcatctg cgcgcgccat gctatcggcg
    44281 taagactcac aatagaaaaa ggcattctgc gctttttcag cgaagtgata caactctgct
    44341 tccgggaaat agggtttttt gtagcggcca aacggcacgt taaactcacc gcgcagattg
    44401 taacgacaca ggccgttgta accgtagcgg ttcaaatata aaaacagtac cgcccgacgg
    44461 aacggatcct ggcttttgtt gaactcttcg cggaactgat agtagacctc ggcgcaattt
    44521 gtttcgggaa caaacagctc gcgtgcggcc tgtacgtact catcagtacg catcttcaca
    44581 atgttataga gactgatcag gtcgctattg atatcggcaa ggatataacg agaaaagtcg
    44641 gtgttgagaa acaccgaccc ggcacctaca aaaggctcaa ccagacattc gcccttgggc
    44701 aaatgccgtt taatatcatc aagcagggga tacttgcccc ctgcccactt caaaaaagcg
    44761 cgatttttct tcatgctgac taactaatta caccttctcc ggctgtggag aaagctccga
    44821 cagcatccag cgcttcaggc aactcccgca acgataaccc attgcgggag ataaccttga
    44881 ttacttcaga tcggcctgta cctgacgcag cggtttcgcc cacgggtttt tggcctgaac
    44941 atctgctggc aatgtagata ccgctttttt cgcctcttct ttcgaagcgt acacgccaga
    45001 aaccaggaca taccacggct gaccattacg cgtcgtttca tagacaacgt agtttttcag
    45061 attctctttc ttcgcccaac cgttcaggtt gtcgtagtta gaggaactgc tcagctgcag
    45121 agtgtaatgg ctggacggtg ccgatttcaa cgaaccaaca ttacctgcgg tcttccctcc
    45181 agcggctggt gttgccgtgg tttgcgccgg ggatgccgtc tgtactggag ccgtagtcgc
    45241 cgtctctttt ggtgctggcg cagaagtcgc agccggtgcc ttcgtgctcg ccacaggagc
    45301 agctggttca gtacgcttcg gcgtctgtgc taccggcttc ggttccgttt tcacggttgc
    45361 ttgcggtttt ttcggttcaa tcaccgcctg ctgacgagct ggacgcgtag tggccggacg
    45421 ttcagcagtt tgcgttttcg ccgtgtcacg cgatgcattg ccattgcgta ctggcgcgac
    45481 agttgctggt tcagtcggca atgtagaatt gaccgccaca ttgttcaact gttgctgatt
    45541 ttgtggctgg gtcagcgcat tgttcaggtc accctgcact tcaacacgtt gttgaccatc
    45601 cgttgccgcc ggggtttgcc cttgagtcgg cgtagaagag atcggtggca gagaaacatc
    45661 ctgctgagta ttttccgcag acgtggttcc cggcgctggc tgcaccccat tcgcctgatc
    45721 ggtcgcattg cctgcaagat caatactctt ctcgccagac gcggtttgat cgctggaaga
    45781 ggtcgagggg gcttttagcg cagaaccgat accgatgatc aacagcagta gaaccagaat
    45841 gccgacgccc atcatcatat actgacgaga agcgggttta ctggctgctt ttttgcgctt
    45901 acgcggacga cgctctacgc gctcttcatc cacggtttca tcttcggatt cgtcaatttc
    45961 ttcttcgatt tccggttcct cattgcgctc tttttgcgca cgagtcggac ggcgatcgtc
    46021 agtgtcatca agttcaatat catcaaaatt gatctgcggt tcgccacgtt cagtacgctc
    46081 agaacgttca gaagattgac gagaacgacc agtacgacga tcgctgggat cgggtttcag
    46141 ctcgtcttct ggtttgaatt catccattta acaccccact aaaaggttaa tgcttaccac
    46201 gttgcaatta acctgaagct aatagaccgc ttgataagcg gcctgacctt tcttgttgtt
    46261 acgctgattg acaatcggca atggcgttaa gaacaagctc gtgcgaaacg ccgctgcgaa
    46321 cttcactctt accaattgcc aacggaagaa ttaagcgtat ctctcccgca aggactttct
    46381 tgtcacgcag catatgcggt aaatacgcct gcgcggacat ttcgcgcggc ccattgaccg
    46441 gtaacccagc ccgcttgagc agggttataa tacgctgcgt ttcggcagaa ctaaactgcc
    46501 cgagacgttc cgacgtccgc gccgccatca ccatacccgc agcgaccgct tcaccatgta
    46561 accaattgcc ataccccatt tcagcttcaa tggcatgacc aaaggtgtgt cccagattca
    46621 gtaaagcacg taacccggtt tcgcgctcgt cggcggcgac aacttctgcc ttcagttcac
    46681 aacaacggcg aatacagtac gccattgccg gaccgtccag acgcaacaac gcatccagat
    46741 tctcttccag ccagttaaaa aacgcaccgt caagaataat gccgtatttg atgacttctg
    46801 ccagccccga cgctaactca cgcgggggaa gcgttttcag acagtcgaga tccaccacca
    46861 ctgaagcagg ctggtagaac gcgccaatca tgtttttacc gaggggatgg ttgaccgcag
    46921 ttttgccgcc aacggaggaa tcgacctgcg acagtaacgt cgtcgggact tgaatgaaac
    46981 gaacaccgcg ctgataactc gccgccgcga agccggtcag atcgcccact acgccgccgc
    47041 caagcgccac cagcgtagta tcgcgaccat gcggcttttg taacaacgcc gtaaagacgg
    47101 tatcgagtac agccaggctt ttatactgct cgccgtcagg gaggataacg ctatcgacgt
    47161 taacacccgc ctgttcaagt acgccgcgga ccttatcgag atacagagga gccagggttt
    47221 cgttggtgac caacatgacc tgctcgcccg atttcagcgg taagaatgaa gctggttcat
    47281 taaacaaacc agatgcgatg gtaattgggt aactacgttc cccgagagta acgacaatcc
    47341 tctccataac gcgacatcca ccttaattac tgtacccgca gacgagtgta tataaagcca
    47401 gaattagttg ctttccagca tgtgaataat ctggtttgca accactttag cgctttgatc
    47461 atcagtacga atggtcacgt cggcaatctc ttcatacagc ggattgcgtt cattggccaa
    47521 cgcttccaga acttcacgcg gcggtgtttc aacgtgcagc aacgggcgtt ttttatcacg
    47581 ctgcgtgcgt gcaagttgct tttcgatggt cgtttcaaga taaacgacaa cgccacgagc
    47641 ggaaagacgg ttacgcgttt cacgggattt cacagagccg ccgccagtag ccagcacaat
    47701 accctgtttc tcggtcaact cattgatgac cttttcttcg cgatcgcgga agccttcttc
    47761 gccttctaaa tcgaaaaccc agcccacatc agctccggtt cgtttctcaa tctcttgatc
    47821 ggaatcgtaa aattccatat tgagttgttg agctaactgg cgcccaatag tgctttttcc
    47881 ggcacccata ggcccaacca gaaagatatt gcgtttctct gccatttttt cggtactact
    47941 aagactattc gttaatgata aacccgcttc gctcagagag cgccgcagga catgaactga
    48001 aacctcataa gatattgcga gagtcagact gaaaattatc tcaatactcc agcgggtttg
    48061 gcaactgaat aaatcaccca tccctctgca tatctggtcg ctgcaagcgc gctgccttgc
    48121 taccaccgct ctggcgataa atcaccgggt aagattagcg taaaaaagac agcaaaatgc
    48181 cgcctgaatg ataaatcatc atcatggaac gcaggcagcg aagagcacca actctcaaat
    48241 cggtactcct tgtatgctaa atacctgcgc gcgtcaaata gatgaaacac gttcagcata
    48301 aaaacattac cgcttttacg gctgtttact cactggaaac cagtcgtggc gtgataaaca
    48361 ccactaactc gcgtcgttca tcttcttttc cgtcatgacg aaataattgc ccgaaccagg
    48421 gaatgtcgcc aagcaacggt acgctatcct gacccgattt atttttacgg gtaaaaatgc
    48481 cgcccagcgc caacgtttct ccgcttttga cctcgacctg cgtttcgatc tcctgcttat
    48541 caatcgccag cacttcgcca tcggcctgct gtagcacctg ccccggaacg ttctggctga
    48601 tgtgtaattt cagccggatg cgaccttttt gtaacaccgt gggcgtgacc tccatcccca
    48661 ggacggcctc tttaaattcc accgacgtcg cgccactttc cccgctggaa acctgatatg
    48721 gaatttcgct cccctgttta atgctggcag gctgaagatg tgaggccagc agacgcggac
    48781 tggcgataat atccagctgc tgtttttgtt cgagcgcgga aagctcaaga tccagcaagc
    48841 gtccgttgat gcgcccaatg ttaaaaccga catgcgttgt cgccgtcgct acggagaggt
    48901 cgctaccaag cgtggtgact tgcccaacgc caccagcgtg ttgcgcatcg gccagcgtcc
    48961 atttcacgcc taactcacgc aaactttttt cattaatggt gacaatatgc gccgacagct
    49021 caacctgccc gaccggcaga tccatttgcg ctacccactg ttcaagcgcg cttaacgccg
    49081 ttttgttatc tcgtagcaaa aggcgattgg tgcgtttatc gacggtcata ctccctttgg
    49141 cactcagtag cttctccccc gctttcgcca gttctcccgc gtcggcgtat tgcagggtta
    49201 tactgcgatt ttccagcggc agatttgcct gcgcccgcgc ctgctccgcc tcctggcggg
    49261 cgatattgtt attctgccag gcaatggaat gcactgagag aatgttgtct tcctgccgcg
    49321 ttatcagtcc ggcgcttttc actacagttt gtagtgcctg cttccaggga acatctgtta
    49381 gatgtaacga caccgtaccg ctgacgtctg gcgacacgac caggttcaac ttctcctgtt
    49441 cagccagcgc ctgcaacacc tgagctaccg gaacgtcatc caccatcagc gtcacttttt
    49501 gcggctttgc cgcctgtacg ccgggtatca gcatcaacag tagtgcggct atccattgct
    49561 tcatttgtat ctccttgccg ttgccacaac cattgtggcg gttcgcagtt tgtcccggta
    49621 cccagcgtta gtacgtctgg cgtcagctgt aaaattgtcc agccgttttc cagcacatcg
    49681 ttttgctgca cccgtcgcca tttcttttgc ccgtctttta ttacaccgat gatgcgctcg
    49741 cctcgcccta ccatcccctg atagcgccac tggctaagtt cgctaatccg gcatagatct
    49801 tccggcggtt taaaagggtc acgcataccg gttaaaaggc acaatgcaat acctgccaac
    49861 aaccagcgtt taaccctcat gcggcgtctc cagttgtagc gtgaacaaaa gatcatcacc
    49921 ttccacgctt aacgaaaaac ggctcacgct gacgttgcgc tctgccagcc gtgtaaatgc
    49981 cgacggcact gcttcccaca gcgttttcaa cgccaactcg cctccctgcg cggatggatg
    50041 ccaggaaacc agttgcgcgc cggataactg aaaatccagt ggcgaaaagg gcagcgtttt
    50101 ttcctcgctg aagggagtgg tgtctaccag gcgatacagt gcggcccact gctggtgatg
    50161 acttgcccgt agtcgaatta atgcgtcgcg ctcttcatgg tgtgtcgatg atagaaaaat
    50221 gagcgtaact aacatcagca accagactgc ccagcaaagc tggcggaggc ggggtgatgt
    50281 ggcgaaccac cagtcaaaga acatgttcat cgctaacctt ccttgttaac tgatactcaa
    50341 attgccagcg tccctgcgca tcctgctgcg tggctccccg ctgattgaga tgaaaagacg
    50401 catcctggcg gagtgacgtt tctagtgcgt ttaacgcggt aatgcttgtt gtcagcccct
    50461 tgatctccag cgttccctgc tgccagctta tcgttgtcag ccaggcgtgc tcgggtaaaa
    50521 gcgccgccag tgcttccagc gcagattgcc agtcgcgggt aaattgtcgc tggcgctgcc
    50581 gttgcgaacg ctgttcgcgt aactgctgtt gctccagcaa acgtggcttc gttatctgta
    50641 agctgcgggc gagttgttgt tccgcctgaa gcaaaactgc gtctatgcgc gcttcggcgc
    50701 tgcctgtcag acgcagtatt agcgttatcc cgacggccag cagcagaggc gcaacgaaca
    50761 tcagcaacca gaaacgcaga aaagcggtcc ggcgttgctg tcgccagggc aaaaaattaa
    50821 ttggcgggtt catcagtacg ccttcccaag cgccagcccc agcgcgatgg caaagtctcc
    50881 acctgacggc ggtagcggcg gctgacgaac agaaacggcc tcccagggat caaatccccc
    50941 ttcgccacat atcgcgacgc tttcgggatc aacggataac gctgccgcca gctccttcgc
    51001 gctagtcatc cccaccgcca gtttgcgccc ccagcgatag cgtgtcgccc acagccactg
    51061 ttcgttatca cgccaggcca gacattgctg atgagaaggt aaaaaaggca ggaatcgctg
    51121 taatgcactg gcatccgggg tgatcgcact aacatgaaca cgcaaccttt ctgccagcgt
    51181 aagcagcgtt gccagctctt tgctttgcgc ggcagtcacg ttataagcgg ggctgagtga
    51241 gtcttcgcta taatcgaagc gcagggagtc cggatccata tccagctcgc gggccatcgt
    51301 ccctgacagc caggccgttt gctcccgctc accaagggac atcgacgggc gcggaaatga
    51361 ccgctgtaat gtgcgactgg cgggaaacgc caacataatg tgatgacgct gcggcagttc
    51421 gcgactccaa ggtaacaacg ttttagccag ctgctgcgca tcaacaatcc gcccatcttt
    51481 gataatgtcg ttctccagcg gcaaccgcca ccagcgttgc aaaaagcatt cttttgcgcc
    51541 ccgtacgatc gcaaccgcta ccgcttcttg ctgttgtaaa tgcaaaccaa tttgccagat
    51601 cttaaatgcc attgtgatga tctccttatc acccgtcact ctgacgggta tatcaatgcg
    51661 tctggcttgc ctttatacta ccgcgcgttt gtttataaac tgcccaaatg aaactaaatg
    51721 ggaaatttcc agtgaagttc gtaaagtatt ttttgatcct tgcagtctgt tgcattctgc
    51781 tgggagcagg ctcgatttat ggcctatacc gctacatcga gccacaactg ccggatgtgg
    51841 cgacattaaa agatgttcgc ctgcaaattc cgatgcagat ttacagcgcc gatggcgagc
    51901 tgattgctca atacggtgag aaacgtcgta ttccggttac gttggatcaa atcccaccgg
    51961 agatggtgaa agcctttatc gcgacggaag acagccgctt ctacgagcat cacggcgttg
    52021 acccggtggg gatcttccgt gcggcaagtg tggcgctgtt ctccggccac gcgtcacaag
    52081 gggcgagtac cattacccag cagctggcga gaaatttctt cctcagtcca gaacgcacgc
    52141 tgatgcgtaa gattaaggaa gtcttcctcg cgattcgcat tgaacagttg ctgacgaaag
    52201 acgagatcct cgagctttat ctgaacaaga tttacctcgg ttaccgcgcc tatggtgtcg
    52261 gtgctgcggc acaagtctat ttcggtaaaa cggtcgacca actgacgctg aacgaaatgg
    52321 ctgtgatcgc cgggctgccg aaagcgcctt ccacctttaa tccgctctac tcaatggatc
    52381 gtgccgtcgc gcggcgtaac gtcgtactgt cgcggatgct ggatgaaggg tatatcaccc
    52441 aacaacagtt cgatcagaca cgcactgagg cgattaacgc taactatcac gcgccggaga
    52501 ttgctttctc tgcgccgtac ctgagcgaaa tggtgcgcca ggagatgtat aaccgttatg
    52561 gcgaaagtgc ctatgaagac ggttatcgca tttacaccac catcacccgc aaagtgcagc
    52621 aggccgcaca gcaggcggta cgtaataacg tgctggacta cgacatgcgc cacggctatc
    52681 gcggcccggc aaatgtgctg tggaaagtgg gcgagtcggc gtgggataac aacaagatta
    52741 ccgatacgct gaaggcgctg ccaacctatg gtccgctgct gcctgccgca gtcaccagcg
    52801 ccaatcctca gcaagcgacg gcgatgctgg cggacgggtc gaccgtcgca ttgagtatgg
    52861 aaggcgttcg ctgggcgcgt ccttaccgtt cggatactca gcaaggaccg acgccgcgta
    52921 aagtgaccga tgttctgcaa acgggtcagc aaatctgggt tcgtcaggtt ggcgatgcat
    52981 ggtggctggc acaagtgccg gaagtgaact cggcgctggt gtcgatcaat ccgcaaaacg
    53041 gtgccgttat ggcgctggtc ggtggctttg atttcaatca gagcaagttt aaccgcgcca
    53101 cccaggcact gcgtcaggtg ggttccaaca tcaaaccgtt cctctacacc gcggcgatgg
    53161 ataaaggtct gacgctggca agtatgttga acgatgtgcc aatttctcgc tgggatgcag
    53221 gtgccggttc tgactggcag ccgaagaact caccaccgca gtatgctggt ccaattcgct
    53281 tacgtcaggg gctgggtcag tcgaaaaacg tggtgatggt acgcgcaatg cgggcgatgg
    53341 gcgtcgacta cgctgcagaa tatctgcaac gcttcggctt cccggcgcaa aacattgtcc
    53401 acaccgaatc gctggcgctg ggttcagcgt ccttcacccc aatgcaggtg gcgcgcggct
    53461 acgcggtcat ggcgaacggt ggcttcctgg tggacccgtg gtttatcagc aaaattgaaa
    53521 acgatcaggg cggcgtgatt ttcgaagcga aaccgaaagt agcctgcccg gaatgcgata
    53581 ttccggtgat ttacggtgat acgcagaaat cgaacgtgct ggaaaataac gatgttgaag
    53641 atgtcgctat ctcccgcgag cagcagaatg tttctgtacc aatgccgcag ctggagcagg
    53701 caaatcaggc gttagtggcg aagactggcg cgcaggagta cgcaccgcac gtcatcaaca
    53761 ctccgctggc attcctgatt aagagtgctt tgaacaccaa tatctttggt gaaccaggct
    53821 ggcagggtac cggctggcgt gcaggtcgtg atttgcagcg tcgcgatatc ggcgggaaaa
    53881 ccggtaccac taacagttcg aaagatgcgt ggttctcggg ttacggtccg ggcgttgtga
    53941 cctcggtctg gattggcttt gatgatcacc gtcgtaatct cggtcataca acggcttccg
    54001 gagcgattaa agatcagatc tcaggttacg aaggcggtgc caagagtgcc cagcctgcat
    54061 gggacgctta tatgaaagcc gttcttgaag gtgtgccgga gcagccgctg acgccgcctc
    54121 cgggtattgt gacggtgaat atcgatcgca gcaccgggca gttagctaat ggtggcaaca
    54181 gccgcgaaga gtatttcatc gaaggtacgc agccgacaca acaggcggtg cacgaggtgg
    54241 gaacgaccat tatcgataat ggtgaggcac aggaattgtt ctgattaaaa aggcgcttcg
    54301 gcgccttttc agttggctga caaagtgcat ttgtttatgc cggatgcggg tgaacgcgtt
    54361 atccggctaa caaaatcgtg aaaaatcaat atattgcagg aaacccataa gcctgataag
    54421 cgttgtgcat caggcaaact tcacgcattt acactcgccc ctgccctttc aaccattcgc
    54481 gcacgaggaa cagcgcgctg acattacgcg cttcgttgaa gtcagggtct tccagcaaat
    54541 ccatcatatg cgccagcggc cagcgcacct gtggtagcgg ctctggctca tcgccttcca
    54601 gcgattccgg gtagagatct tgcgcgacca cgatattcat tttgctggaa aagtaagacg
    54661 gtgccatgct gagcttcttc aaaaaagtca gatcgttcgc accaaatcca acctcttctt
    54721 ttagctcgcg gttagcggct tcgtagacgc tttcacccgg atcaattaat cctttcgaaa
    54781 aacctaattc gtaggattca gttcccactg cgtattcgcg gatcaggatc aggtgatcgt
    54841 ccacaatcgg cacaatcatc actgcttccc ggttggttgg acgcattcgt tcataaacac
    54901 gccgcacgcc attgctgaac tccagatcca cgctctcgac ggtaaacagt cgggaacggg
    54961 ctacagtttc aacattcaga atggtgggtt tttgtaatga tttgctcatc gtgggatcta
    55021 tgctgtgaaa tcagcggtta ttgtgcgata tcggacacgc tttcggcaat gtgaattgca
    55081 tgttatttac atttatgtaa cttaataaat aatcgtcctc aaatcaaatt aaaagtcaat
    55141 aggttgaaat aactccagga atttgctgat attccgcctt cagagggttt gctatgatca
    55201 gcggttactg tgatgtgctt aatgatgctc aagttaaact ccacgcttgc cgatagccaa
    55261 ccgcagaatc atgtattgtg tctggtgcga ctgaccacgc ctgacagact aagtaagatg
    55321 gggaaagcat gagcaccatt gtgatttttt tagctgcttt gctggcctgc tcactacttg
    55381 cgggatggct gataaaagtg cgatccagac ggcgtcagct gccctggacc aacgccttcg
    55441 cggatgcgca aacgcgtaaa ctcacacctg aagaacgtag cgccgttgaa aattatcttg
    55501 agagcctgac gcaggtatta caggtgcctg gcccaacggg agctagcgcg gcaccgatct
    55561 ctctggcgct gaatgccgaa agcaacaacg tcatgatgct gacacacgct atcacgcgtt
    55621 acggcatctc taccgacgat ccgaataaat ggcgttacta cctcgattcg gtagaagtcc
    55681 acctgccccc tttctgggaa caatacatca acgatgagaa taccgttgaa ctgatttata
    55741 ccgattcgct gccgctggtt atttcactca acggtcatac gctgcaggag tacatgcagg
    55801 aaacgcgcgg ctatgccttg caacctgttc cgtccacgca ggcgtcgatt cgcggggaag
    55861 agagtgagca aatcgagcta ctcaatattc gcaaagaaac gcatgaagaa tatgcgctga
    55921 gtcgtccgcg cgggctgcgt gaagcgttgc tgatcgtcgc ctccttcctg atgttctttt
    55981 tctgcctgat taccccggat gtatttgttc cgtggctggc aggcggcgca ttactgctgc
    56041 tgggcgcagg tctgtggggg ctattcgcgc ccccggcaaa atcctccctg cgagaaattc
    56101 attgtctgcg cggtacaccc cgtcgttggg gattgtttgg cgaaaacgat caggaacaga
    56161 tcaacaatat ttcactcggt attatcgacc tggtctatcc cgcacactgg cagccataca
    56221 ttgctcagga tctcggtcaa caaaccgata ttgatatcta tctcgaccgc cacgtagtgc
    56281 gtcaggggcg atatctttcg ctgcatgatg aagtaaaaaa ctttccgtta cagcactggc
    56341 tgcgcagtac gattatcgct gcgggctcgc tgctggtgct gtttatgctg ttattctgga
    56401 tcccgctgga tatgccgctg aaattcactc tctcatggat gaaaggcgcg cagaccattg
    56461 aagccaccag cgtaaaacaa ctggctgacg ctggcgtgcg ggtaggcgat acattgcgta
    56521 ttagcggtac gggaatgtgt aatattcgca cttccggaac ctggagcgcg aaaaccaatt
    56581 cacctttttt accgtttgac tgctcgcaga tcatctggaa tgacgcccgc tcattaccat
    56641 taccagaatc tgaactggtc aacaaagcga cggcattgac tgaagcagtt aatcgccagc
    56701 tgcaccctaa accggaagat gaatctcgcg tcagtgcctc attacgttca gcgattcaaa
    56761 aatccggcat ggtattgctt gatgattttg gcgacattgt actgaagaca gcggatttat
    56821 gttctgccaa agatgactgt gtgcgactga aaaatgcgct ggtcaatctc ggcaacagta
    56881 aagactggga cgcgctggta aaacgcgcca acgccgggaa gctcgatggc gtgaatgtgt
    56941 tattacgccc ggtgagtgcg gaatcgctgg ataacctggt ggcaacctcc accgcgccgt
    57001 ttatcacgca tgaaacggcg cgagcggcac aatcactaaa cagtccggcc cccggcggat
    57061 tcctgattgt cagcgacgaa ggcagcgatt ttgttgatca gccctggcct tcggcatcac
    57121 tttacgacta cccgccgcaa gaacagtgga acgctttcca gaaactggca caaatgctga
    57181 tgcatacgcc gtttaacgcc gaaggtatcg tcacaaaaat cttcactgac gccaatggta
    57241 cgcagcatat tggccttcat ccgatcccgg atcgttccgg cctgtggcgc tatctcagca
    57301 ccacattgct gctactgacg atgctgggta gcgccattta caatggcgta caggcctggc
    57361 gtcgttacca gcgtcatcgc actcgcatga tggagattca ggcctattat gaaagctgcc
    57421 tgaacccgca actgatcacc ccttcagaaa gccttatcga ataacacgtt tgcgcggcag
    57481 gttatgctac cctgtcgcgc aaattgcttc actctggaga tttccctcat gcatatcaac
    57541 attgcctggc aggacgtaga taccgttctg ctggatatgg acggcacatt gctcgacctc
    57601 gccttcgata actatttctg gcaaaagctg gtgcctgaaa catggggcgc gaaaaacggg
    57661 gttacgccac aggaagcgat ggaatatatg cgccagcaat atcacgacgt acagcatacg
    57721 ctaaactggt actgtcttga ttactggagt gagcaactgg gtctggatat ctgtgcgatg
    57781 accaccgaga tgggaccgcg tgccgtactg cgtgaagata ccattccgtt tcttgaggca
    57841 ctgaaagcca gcggtaagca gcgaattttg ctcaccaatg cgcatccgca caacctggcg
    57901 gtaaaacttg agcataccgg tctggacgca caccttgatt tattactttc cacccacaca
    57961 tttggttatc cgaaagagga tcagcggtta tggcatgcgg tggccgaagc tacgggtctg
    58021 aaagctgaaa gaacgctgtt tattgatgac agcgaagcga ttctcgatgc tgccgcgcaa
    58081 tttggtattc gttactgcct cggcgtgact aatcctgatt ccgggattgc cgagaaacag
    58141 tatcaacgcc atccgtcact gaatgactac cgccgcctga tcccctcgct aatgtgaagg
    58201 agacgccatg aaagaaaaac ctgctgttga ggttcgactg gataaatggc tatgggctgc
    58261 ccgtttttat aaaacccgcg cgctggcccg tgaaatgatt gaaggcggta aggtgcatta
    58321 caacgggcag cgcagcaagc cgagcaaaat cgtcgagctg aatgccacgc tcactctgcg
    58381 ccagggaaat gacgaacgca cggtgattgt aaaggcgatt actgaacagc gtcgccccgc
    58441 cagcgaggca gccttgctgt atgaagagac tgcggaaagt gtagagaaac gcgaaaaaat
    58501 ggcgctggca cgtaaactta atgccttaac catgccgcac ccggaccgac gcccggacaa
    58561 aaaagagcgc cgcgacctgt tacgatttaa acacggcgac agtgaataac tgtcacctgc
    58621 aagagagatg attatgccgc aacatgacca attacatcgc tatctgtttg aaaactttgc
    58681 cgtgcgcggc gaactggtaa ccgtttcgga aaccctgcaa cagatccttg agaatcacga
    58741 ttatccgcag cccgttaaaa acgtgctggc agaactgctg gttgcgacca gcctgttaac
    58801 cgctacgctg aagtttgatg gtgatatcac cgtacagctg caaggcgacg gtccgatgaa
    58861 tctggcggtt attaacggta acaataacca gcaaatgcgc ggtgtggcgc gcgtgcaggg
    58921 cgaaattcca gaaaatgccg acctgaaaac gctggtcggc aatggttacg tggtgatcac
    58981 cattaccccg agcgaaggcg aacgctatca gggcgtggtt ggtctggaag gtgataccct
    59041 ggcggcctgc ctggaagatt actttatgcg ttctgaacag ctgccgacgc gcctgtttat
    59101 tcgcaccggc gacgtagacg gcaaaccggc tgcaggcggt atgttgttgc aggtaatgcc
    59161 tgcgcaaaat gcccagcagg acgactttga ccacctggcg acgctaaccg aaaccatcaa
    59221 aaccgaagaa ctgctgacct taccggcaaa cgaagtgttg tggcgtttgt atcacgaaga
    59281 agaggtgacg gtttacgatc cgcaggatgt ggagttcaaa tgcacctgct cgcgtgaacg
    59341 ttgcgccgat gcgctgaaaa cgctgcctga tgaagaagtt gatagcatcc tggcggaaga
    59401 tggcgaaatt gacatgcatt gtgattactg cggtaaccac tatctgttca atgcgatgga
    59461 tattgctgaa atccgcaaca acgcgtctcc ggcagatccg caagttcatt aatgagtatg
    59521 tccggcagag agggctctgc cggattttac gacgtaatgg cctgatgtgc tgcgcttacc
    59581 aggcctgtga aataaatcgc aaattattgt tggtcagata cagcgtcgta tccgttaaaa
    59641 gagttgtcac ttacttaagt ttatttccag cgtttcacca tctaccgtca gatcgcgcca
    59701 cgctttcggc ctcgtcccca gttttccatc cttcgcagct gtgagtaatt tttctttcgt
    59761 tatttcgttc ggaagtaaac tcatatcaag cgcgtcccat tcttttctgt ctgagtcaaa
    59821 gccatagaca atgactctgt catcattaaa agcaaacagg atccgctccg cctgaccatc
    59881 actattcaga tcttgctcga ccaggacgca ggcgtctttt tcaatacagg tcatcacgtt
    59941 atagcgatcc tggattaagg ccgaccagaa tgtcgcatcc ggtttaacag aaccaggggc
    60001 aattaacaca ttttcggcta atactttttc cgatacctgt tcctgaagat gttgctctcc
    60061 atctaacgcc atcagcagat cccgcgcgcg tttcgggtct ttcataaacc cggcatcgct
    60121 tttcagcgac tcaagcgccg cacgtccata gcgaccgctc tgttcgagca tgtagatagt
    60181 tacctggtct ggcgtgtttt tgccgctctg ataacgcgcc atatgactgt tcacgctaat
    60241 acgcatactg tccagcaccg gcgaattaag aagcaccagt atcaccaaca ccaataacga
    60301 aaccgcaagg ttcactttgc cctgaagaac aaggggattt tgcccattac gccagacgat
    60361 actgacaaaa taccccagcg accacaccag taacaccagc accgccagcg cgccctgcaa
    60421 gcggtcgaca gtccagccgt actgagcgac ccgcagccat aacgcccagg cggcgacgaa
    60481 cacatacagc ggagcaacca gcaaagcggt tttaatcagg caacgcaacg gccccgtcca
    60541 gggaagtgac gctttttgcg gatcgcggac aatagccatt aagatcaatt gcaaaaaggc
    60601 cagcgttaac agcaacccgg cggcggagat gtggcgagaa atcgcgctca ggcccgtaaa
    60661 cggcagagtg atgataaaca tcagggttag caatgatact aacggcagca accccgtggc
    60721 gattaacgtg aacaactttt gaatagagtc gattaaacgt gactgtgtgc gcgcgagaat
    60781 caccgccagt gcggtaacca gacctaatgt aagatacata aaccagtcgg ttgcaaaaaa
    60841 gagcgtattg aaaaacttga tgccaacaag tttaaacaac tcaccccaca gtaaaagcac
    60901 cagccacgtc aggccattgg cgagaaaaat caccagtaat ataagtacgt tatgccatac
    60961 tgactggtaa aagtagctat agcggctact gccattgcag atacgaaggc tttgttgtat
    61021 ccacggtagc aacaacattg ccatcaacag cagatagcag ccaaaatccc aaatcgcctt
    61081 ttcagctctc caggggttca tgccatcagt ttgccacttc agccaaccgc tcatccctgt
    61141 cgtggcaata aacaccagcg ccagccatcc ccaaaggcgc ttctgtttaa aagagatcac
    61201 ggagaagagt aaaacggatg aaaacgcaac ggtcgcaggc acgccataaa caatccaact
    61261 gtgatttttt ccggcaagcc aggcgatcaa caggtagcaa accagcccct gaagtaatcc
    61321 ggtagcaatc atcccccagc gtgttgccgg tgaaagttca acgttatcca tgttggttat
    61381 ccagaatcaa aaggtgggtt aattatcgca tccgggcagt agtattttgc ttttttcaga
    61441 aaataatcaa aaaaagttag cgtggtgaat cgatacttta ccgattgaat ttgcatcaat
    61501 ttcattaagg aatgcgattc cactcacaat attcccgcca tataaaccaa gatttaacct
    61561 tttgagaaca ttttccacac ctaaaatgct atttctgcga taatagcaac cgtttcgtga
    61621 caggaatcac ggagtttttt gtcaaatatg aatttctcca gatacgtaaa tctatgagcc
    61681 ttgtcgcggt taacaccccc aaaaagactt tactattcag gcaatacata ttggctaagg
    61741 agcagtgaaa tgcgcgttaa caatggtttg accccgcaag aactcgaggc ttatggtatc
    61801 agtgacgtac atgatatcgt ttacaaccca agctacgacc tgctgtatca ggaagagctc
    61861 gatccgagcc tgacaggtta tgagcgcggg gtgttaacta atctgggtgc cgttgccgtc
    61921 gataccggga tcttcaccgg tcgttcacca aaagataagt atatcgtccg tgacgatacc
    61981 actcgcgata ctttctggtg ggcagacaaa ggcaaaggta agaacgacaa caaacctctc
    62041 tctccggaaa cctggcagca tctgaaaggc ctggtgacca ggcagctttc cggcaaacgt
    62101 ctgttcgttg tcgacgcttt ctgtggtgcg aacccggata ctcgtctttc cgtccgtttc
    62161 atcaccgaag tggcctggca ggcgcatttt gtcaaaaaca tgtttattcg cccgagcgat
    62221 gaagagctgg caggtttcaa accagacttt atcgttatga acggcgcgaa gtgcactaac
    62281 ccgcagtgga aagaacaggg tctgaactcc gaaaacttcg tggcgtttaa cctgaccgag
    62341 cgcatgcagc tgattggcgg cacctggtac ggcggcgaaa tgaagaaagg gatgttctcg
    62401 atgatgaact acctgctgcc gctgaaaggt atcgcttcta tgcactgctc cgccaacgtt
    62461 ggtgagaaag gcgatgttgc ggtgttcttc ggcctttccg gcaccggtaa aaccaccctt
    62521 tccaccgacc cgaaacgtcg cctgattggt gatgacgagc acggctggga cgatgacggc
    62581 gtgtttaact tcgaaggcgg ctgctacgca aaaactatca agctgtcgaa agaagcggaa
    62641 cctgaaatct acaacgctat ccgtcgtgat gcgttgctgg aaaacgtcac cgtgcgtgaa
    62701 gatggcacta tcgactttga tgatggttca aaaaccgaga acacccgcgt ttcttatccg
    62761 atctatcaca tcgataacat tgttaagccg gtttccaaag cgggccacgc gactaaggtt
    62821 atcttcctga ctgctgatgc tttcggcgtg ttgccgccgg tttctcgcct gactgccgat
    62881 caaacccagt atcacttcct ctctggcttc accgccaaac tggccggtac tgagcgtggc
    62941 atcaccgaac cgacgccaac cttctccgct tgcttcggcg cggcattcct gtcgctgcac
    63001 ccgactcagt acgcagaagt gctggtaaaa cgtatgcaag cggcaggtgc gcaggcgtat
    63061 ctggttaaca ctggctggaa cggcactggc aaacgtatct cgattaaaga tacccgcgct
    63121 attatcgacg ccatcctcaa cggttcactg gataacgcag aaactttcac tctgccgatg
    63181 tttaacctgg cgatcccaac cgaactgccg ggcgtagaca ctaagatcct tgatccgcgt
    63241 aatacctacg cttccccgga acagtggcag gaaaaagccg aaaccctggc gaaactgttt
    63301 atcgacaact ttgataaata caccgacacc cctgcgggtg ccgcgctggt agcggctggt
    63361 ccgaagctgt gatgatttga agctggataa tatctatcca gtatcttaga gaaagcaaaa
    63421 cgggagacac cttcgcctcc cgttttattt acccttcttt tgtcgtgccc tgcgcccgcg
    63481 ttaccggcac tggcagccag gcgcgaatgg aaagcccgcc ccgctcgctg gtgccaagct
    63541 ccagcatccc gttatggtta tccacgatac gctgcacaat cgccagccct aatcccgtgc
    63601 cgctaatggt gcgcgcactg tcgccgcgaa caaacggctg gaaaaggtgc ttacgctgtt
    63661 ccggcgcaat tcccggaccg tcatcttcca cctggaacca agcgcgattc ggctccgtcc
    63721 cgctgctgac tttgatccag ccattgccgt aacgggcggc attgaccacc atattcgcca
    63781 ccgcgcgttt gatcgacagc gggtgcattt tcacttcaat gctgccgggg taaagcgcgg
    63841 tttcaatttc ccgctcatag ccactttcgg cagcaataac ctcaccgagt actgcgttga
    63901 gatccgccat ttccatcggc atctcctgcc cggtgcgcag gtaatcgata aactgctcaa
    63961 tgatggcgtt gcactcttcg atatctttat tgatcgattc ggccaggtag ccatcctgtt
    64021 cgctcatcat ctcggtcgcc aggcgaatac gcgtcagcgg cgtgcgtaag tcatgactta
    64081 cccccgccat cagcagcgtg cggtcatccg ccagttgctt aacaccagcc gccatatggt
    64141 taaaggcgcg ggtaaccgaa cgcacctccg aagcgccata ctcacgcagc ggcggcggaa
    64201 taatcccttt accgacctgc aaagcagcat gttcgagatc gaccaacggt cggttctgga
    64261 tacgaataaa cagccacgcc ccgcctatcg ccaatagcat aatcgccaac gtatagcgga
    64321 acagcgggga aaaatcgccc tgatgaattt cggtcagcgg cacccgtacc cagatattgg
    64381 gcgacagcca ggttttcagc cagacgacag gcgaactttt gttgacctca acgcgcactt
    64441 ccgtcgggcc gcccagttgc tgtgccatct gatggcttaa gaattcatag tgttgtgccc
    64501 aacgcagacc tgcctcttcg gcagcttcgt tggagtagag agagatcccc agctcacggt
    64561 agatctcccg acggaaagcg ggaggcacaa ccaactgcgt gccgtcctcc agttgcagtt
    64621 tgtcggtcat caacatacgc acttcgtacg cgaggacttt attaaactgc tggaggctcg
    64681 gcaaaatcgc gaagttcagc accaccagat aagtcgtcac caggctggcg aacagcaagg
    64741 tgacgatgag caataacgta cgggcaaatg aacttcgtgg cgagaagcgc aatcgcctca
    64801 tgctttagag ccgtccggta caaagacgta gcccaggccc caaacggtct gaatgtaacg
    64861 cggatgcgcc ggatcttctt ccaccatgcg gcgcagacgt gaaatctgca cgtcgatgga
    64921 gcgttccatt gcggaatatt cacggccacg ggcaaggttc atcagcttat cgcgggagag
    64981 cggctcacgc ggatggctga ccagtgcctt cagtaccgca aactcaccgc tggtgagcgg
    65041 catcggctca tcttcgcgga acatttcgcg cgtaccgagg ttaagtttga acttaccgaa
    65101 agcaattacc gcctcttcct gtgacggtgc gcctggtagc tcgttcgcct gacgacgcag
    65161 caccgcacgg atacgggcca gcagttcacg cgggttaaac ggttttggaa tgtaatcgtc
    65221 agcgccaatc tccaggccaa cgatacggtc cacttcttcc cctttcgccg ttaccataat
    65281 gatcggcatc gggttgctct ggctacgaag acgtcggcaa atcgacaagc catcttcacc
    65341 aggtaacatc aaatccagta ccataagatg gaaagattca cgagtcagca ggcgatccat
    65401 ctgttctgca ttagcgacgc ttcgaacctg gaagccttgt tcggtgagat aacgttccag
    65461 cagcgcacgc aggcgcatgt cgtcatcgac caccagaatc ttgtagttct cttgcattgt
    65521 ttgtactccc aaaggttcgc aacaatttgt aagcgtgtat tcttaaaaaa gctcacgttc
    65581 gtcaccagct aaatctggta tgaatttcag cctaaattgt tacaaagcat attaaacagc
    65641 agcttaagta tacaatttat tcggcgaaac attattgatt ctgttgatat gatcacgtta
    65701 tacccaatgt gcgcattatc aaacagacaa agggaatcaa cgagatgaaa acgcccctgg
    65761 ttacccggga agggtatgaa aaactcaaac aagagcttaa ttatctctgg cgtgaagaac
    65821 gcccggaggt cacaaaaaag gtgacctggg ccgcaagtct gggcgaccgc agcgaaaatg
    65881 ctgactatca gtataataaa aagcgtctgc gtgaaatcga ccgtcgcgtg cgctatctca
    65941 ctaaatgcat ggaaaatctc aaaatcgtcg attactcccc tcagcaggaa ggcaaagtct
    66001 tttttggcgc gtgggtggag attgaaaacg acgatggcgt gactcaccgt ttccgtattg
    66061 tcggctacga tgaaattttt ggccgtaaag attacatctc tatcgattcc ccgatggccc
    66121 gcgcattgct gaaaaaagaa gtcggcgatc tggcggtggt gaatacccct gccggggaag
    66181 cgagctggta tgttaatgct atcgagtacg tgaaaccgta aggaagagtc ttaacctcct
    66241 gccgatggct ggcatttttg ccagccagtc cgtataacta tcccctgatt tttgatccga
    66301 aaagatgaac tcaaaccatg atgaatgatt cgttctgccg cattattgcg ggtgaaattc
    66361 aggcgcgccc ggaacaggtt gacgctgccg ttcgcctgct tgacgaaggg aataccgtgc
    66421 cgtttatcgc acgttatcgt aaggaaatca ccggcggtct ggatgacacg caactgcgta
    66481 atctggaaac gcgtctgagc tatctgcgcg agctggaaga gagacgtcag gcgatcctca
    66541 aatccatttc cgagcaaggc aaactcaccg atgatctggc gaaggccatc aacgccaccc
    66601 taagcaaaac cgaactcgaa gacctctacc tgccctacaa acctaaacgc cgcacccgcg
    66661 ggcaaatcgc cattgaagca gggcttgagc cgttggctga cctgctgtgg agcgatccgt
    66721 cacacacgcc agaagtcgcc gctgcacaat atgttgatgc cgataaaggc gtggcagata
    66781 ccaaagccgc gctggatggc gcgcgctata tcctgatgga acggtttgcc gaagatgccg
    66841 cgctgctggc gaaagtgcgt gattatctgt ggaagaacgc gcatttggtt tctacggtgg
    66901 tgagcggtaa agaagaggaa ggggcgaaat tccgcgacta tttcgatcat cacgaaccgt
    66961 tgtccacggt gccttctcac cgcgcgctgg cgatgttccg tgggcgtaac gaaggcgtac
    67021 tccagctttc gctgaatgcc gatccgcagt tcgatgagcc gcccaaagag agctattgcg
    67081 agcaaatcat catggatcac cttggcctgc gcctgaacaa tgccccggcg gatagctggc
    67141 gcaaaggcgt agtgagctgg acctggcgca tcaaggtgct gatgcatctg gaaaccgaac
    67201 tgatgggtac cgtgcgcgaa cgcgcggaag atgaagcaat caacgtcttt gcccgtaacc
    67261 tgcacgatct gctgatggcg gcccctgccg gactgcgtgc aacgatgggc ctcgatccgg
    67321 gtctgcgtac tggggtaaaa gtggcggtgg tcgatgccac tggcaaactg gtggcgaccg
    67381 ataccattta cccgcacacc ggacaagccg caaaagcagc gatgaccgtt gctgccttgt
    67441 gtgaaaaaca taacgttgaa ctggtggcga tcggcaacgg tacagcttcc cgcgaaactg
    67501 aacgtttcta tctcgacgtg cagaagcagt tcccgaaagt gaccgcacag aaagtgatcg
    67561 tcagcgaagc tggcgcgtcg gtttactcag cttccgagct ggcagcgcag gagttcccgg
    67621 atctcgacgt ttcgctgcgt ggtgcggtat ctatcgcccg ccgtttgcag gatccgctgg
    67681 cggagctggt gaaaatcgat ccgaaatcta tcggcgtagg tcagtatcag catgacgtca
    67741 gccagacgca actggcccgc aaactggacg cagtagtaga agactgcgta aacgccgttg
    67801 gcgtcgatct caacactgct tctgttccgc tgttaacccg cgtggcgggc ctgacgcgca
    67861 tgatggcgca aaacatcgtg gcctggcgcg atgagaacgg tcagttccag aaccgtcagc
    67921 aactgctgaa agtgagccgt ctggggccga aagccttcga gcagtgcgcg ggcttcttgc
    67981 gcattaacca tggtgataac ccgctggacg cgtctaccgt ccacccggaa gcctatccgg
    68041 tggtggaacg cattctggca gcaacacagc aggcactgaa agatctgatg ggtaacagca
    68101 gcgaactgcg taacctgaaa gcgtctgact ttactgatga aaaattcggt gtgccgacag
    68161 taactgacat catcaaagag ctggaaaaac cgggtcgcga tccgcgtccg gaatttaaaa
    68221 ccgctcagtt tgccgatggc gtcgagacaa tgaacgacct gcaaccgggt atgatcctcg
    68281 aaggtgcggt gaccaacgtc accaactttg gcgcgtttgt cgatatcggc gtgcatcagg
    68341 acggactggt tcacatctct tcattgtcga acaagtttgt agaagatccg cataccgtgg
    68401 tgaaagcggg cgacattgtg aaggtgaaag tgctggaagt ggatcttcag cgtaaacgta
    68461 tcgccctgac catgcgtctg gacgagcagc ctggcgaaac caacgcccgt cgcggcggcg
    68521 gtaatgaacg cccgcaaaac aaccgcccgg cagccaaacc gcgcggacgt gaagcgcagc
    68581 ctgccggtaa cagcgcgatg atggacgcgc tggcggcagc aatgggcaaa aaacgttaaa
    68641 cgccctgatt gacccctaaa cctgcgcgat aaggtgtcca cgccgcatct ggcaacgaac
    68701 gccttgtctg acataaaagt gccggagaat atctccggca tttttattcc acagccaaac
    68761 tcacaatata ttccggcaac atttatcact tcattaacaa ctgaaacctt aattaaacat
    68821 tagccagtcc tggtaattcg ctattcgaat tatattttcg ctgcgatata accttgagcc
    68881 acatcaacat tgagtcagat tattattcaa accaacattc gcacacattt taagtattgc
    68941 tgatagaaac cattctcatt atcattgtgc tgttgattat ttaatctctc cttcgttggc
    69001 aaatcatctg gtctcatgtc gctgtcaaac gccccatgag gtagttatcc agttaatgag
    69061 aaacaagtag gcacctatgc aatacactcc agatactgcg tggaaaatca ctggcttttc
    69121 ccatgaaatc agcccggcat atcgccaaaa actgctttct cttggcatgt tacctggctc
    69181 ctcttttaat gtggtgcgcg tcgctccact cggcgacccc attcatatcg aaacccgtcg
    69241 tgtgagcctg gtattacgca aaaaagatct ggccttatta gaagtggaag cggtttcctg
    69301 ttaatacggt gataacaaca atgaaaaaat taaccatagg cttaattggt aatccaaatt
    69361 ctggcaagac aaccttattt aaccagctca ctggcgcacg tcagcgtgta ggtaactggg
    69421 ctggcgttac cgtcgaacgt aaagaagggc aattctccac caccgatcat caggtcacgc
    69481 tggtggacct gcccggcacc tattctctga ccaccatctc atcgcagacc tcgctcgatg
    69541 agcaaatcgc ctgtcactac attttgagtg gcgacgccga cctgctgatt aacgtggtgg
    69601 atgcgtctaa ccttgagcgt aacctgtacc tgacgctaca actgctggaa ctcggcattc
    69661 cctgcatcgt ggcactgaac atgctcgaca ttgccgagaa gcaaaatatt cgtattgaaa
    69721 ttgatgctct gtcggcgcgt cttggctgcc cagtgatccc gctggtttca acccgtggtc
    69781 gcggtattga agcgctcaag ctggcgattg atcgctataa agctaacgag aatgtggaac
    69841 tggtgcatta cgcacagccg ctactcaacg aagcagattc actggcaaaa gtgatgcctt
    69901 ccgacatccc gctgaaacaa cgtcgctggc tgggcctgca aatgctggaa ggcgatatct
    69961 acagccgcgc ctacgccggt gaagcgtcgc agcatctgga tgccgccctc gcccgtctgc
    70021 gtaatgagat ggacgatccg gcgctgcaca ttgccgatgc gcgttaccag tgcattgctg
    70081 ccatctgtga tgtggtaagc aacaccctga cggcagaacc cagccgtttc accactgcgg
    70141 tagataaaat cgtgctcaac cgtttcctcg gtctgccgat tttcctcttt gtgatgtacc
    70201 tgatgttcct gctggctatc aacatcggcg gggcgttaca gccgctgttt gacgtcggct
    70261 ccgtggcgct atttgtgcat ggtattcaat ggattggcta cacgctccac ttcccggact
    70321 ggctgactat cttcctcgcc cagggcctgg gtggcggtat taacaccgtg ctgccactgg
    70381 tgccgcagat tggcatgatg tacctgttcc tctccttcct tgaggactcc ggctatatgg
    70441 cgcgtgcggc gtttgtgatg gaccgcctga tgcaggcgct gggcttgcct gggaaatcct
    70501 ttgtgccgct gatcgtcggt ttcggttgta acgtaccgtc ggtaatgggc gcacgtacgc
    70561 ttgatgcacc gcgtgaacgt ctgatgacca tcatgatggc accgtttatg tcctgcggcg
    70621 cgcgtctggc tatcttcgca gtattcgcgg ctgccttctt cgggcagaac ggtgccctgg
    70681 cggttttctc gctgtatatg ctgggtatcg tgatggcggt gctgactggc ctgatgctca
    70741 agtacaccat catgcgcggt gaagcgacgc cgtttgtcat ggagctgccg gtctaccatg
    70801 taccacacgt taaaagcctg attatccaga cctggcagcg tctgaaaggc ttcgttctgc
    70861 gtgctggtaa agtgattatc atcgtcagca ttttcctgag cgccttcaac agcttctcgc
    70921 tgagcggaaa aatcgtcgat aacatcaacg actcggcgct ggcttccgtc agccgggtga
    70981 tcaccccagt cttcaagccg attggcgtgc atgaagataa ctggcaggca acggttggcc
    71041 tgtttacagg tgcaatggcg aaagaagtgg tggtgggcac gctcaacacc ctctacaccg
    71101 cagaaaatat tcagtacgaa gagttcaatc cggcagaatt taacctcggt gaagagctgt
    71161 tcagcgcggt agatgaaacc tggcagagcc tgaaagacac cttcagcctt agcgtactga
    71221 tgaatcccat tgaagcgagc aaaggcgacg gcgaaatggg taccggggcg atgggcgtga
    71281 tggatcagaa attcggtagc gcagctgccg catacagcta cctgattttc gtcctgctgt
    71341 atgtaccatg tatctcggtg atgggagcca tcgcccgtga atcaagccgt ggctggatgg
    71401 gcttctccat cctgtggggg ctgaatatcg cttactcact ggcaacattg ttctatcagg
    71461 tcgccagcta cagtcagcac ccaacttaca gcctggtgtg cattctggcg gtaatcctgt
    71521 ttaacatcgt agttatcggt ctgctgcgcc gcgcgcgtag ccgggtggat atcgaactgc
    71581 tggcaacccg caagtcggta agcagttgct gcgcagccag caccaccggt gattgccatt
    71641 aatggcttca cttattcagg tacgtgattt gctggcgtta cggggccgta tggaagcgac
    71701 ccagataagc cagacattga acactccgca gccaatgatt aacgccatgc tgcaacagct
    71761 ggaaagcatg ggcaaagccg tgcggattca ggaagaacct gacggctgcc tctctggcag
    71821 ttgtaaaagc tgcccggaag gcaaagcctg tctgcgcgag tggtgggcgc tgcgttaacc
    71881 atactccatc gcctgttttg aaaagcccgg tatgcgtttg catccgggct tttttgcgtg
    71941 cggctttcca taaaaatgca attcttgcag cgcgacgtaa gttcctctga aagcgtctcg
    72001 cagggatgaa aactggctaa tgcacagatg tggagtggcg cgtagagtcg cggcattcaa
    72061 acaacaggtg aaggaacgcc atgagtaaaa agcagagttc caccccacat gatgcgctgt
    72121 tcaaactctt tttacgccaa ccggagacgg cacgcgattt tcttgcgttt catttaccgg
    72181 cacccattca cgcgctctgt gatatgaaaa ccctcaagct ggagtcgagc agctttattg
    72241 atgacgatct gcgtgaaagc tattccgatg tgctgtggtc ggtgaaaacg gaacaaggac
    72301 caggatacat ctattgtctg attgaacatc aaagcacctc aaacaaactg atcgcatttc
    72361 gcatgatgcg ttacgctatt gccgcaatgc aaaatcacct tgatgctgga tacaaaacgt
    72421 tgccgatggt ggtgccattg ttgttttacc acggtattga aagcccctat ccctattcgc
    72481 tgtgttggct ggattgtttc gccgatccca aactggcaag gcaactttat gcctccgcat
    72541 ttccgctgat tgatgtcacc gtcatgcctg atgatgaaat catgcagcac cgacgtatgg
    72601 cgctgctgga gttaattcaa aaacatattc gtcaacgcga tctgatgggg ctggtagagc
    72661 aaatggcctg cttattaagt agtggatacg ctaatgacag acaaatcaaa gggctgttta
    72721 attacatact gcaaactggc gacgctgtac gttttaacga ttttatcgac ggcgttgccg
    72781 aacgttcacc gaaacacaag gagagtttaa tgactattgc ggaaagattg cggcaggagg
    72841 gggaacaatc caaagccctg catatagcca aaataatgct tgaatccgga gtccctctta
    72901 cagacatcat gcgctttacc ggggtgtcag aagaagagtt ggctgcggcg agccagtaaa
    72961 tttctgtctc gccatttcaa aagttgccta caccctctgc ttcaacgcca ccagcaggtg
    73021 acaaaactcg accggatgcg aaataaacgg cgcatgggcc gctttggcga agatatatga
    73081 ttcgctgtga ggccaaagtt tatccagcat cggcaccact ttgcgcggca ccagaccgtc
    73141 gagatagcca tacaatcgca aaaacggcat ggacacgttt tgcagcggct gacggagatc
    73201 gaccgttttc aggatttcca gcccgccatt aagcacgtca acctccggca tcggtaacgc
    73261 cagaacggtt ttcttcaacg cccgcgcatc ctggcgcgcc gtttcagtcc ccatggtttg
    73321 taacgccagg aaccgctcca ctgtacgctg aaaatcatca ctgagttgct gctgaaatcc
    73381 cgccagcacg tccggtttta tccccggcca ctcgtcacga gcactaaaac aaggtgacga
    73441 cgccacggtg accagcgcct gaacacgctc gggatgggtt aacgcaatct ggcttgccac
    73501 cagcccgccc agactccagc ctaaccaaat ggctttatca ggtgcctgtt gcagcacggc
    73561 ttcggccata tcagcaagtg acagcgcacc aaatccccgg ctacgcccga agccgggcag
    73621 gtcaacaagg tgcagcgtaa aatgcgagct aagttcctcg tcaatgcaac accacacttc
    73681 ggcattcagt ccccatccgt gcagcagcac aagatgaaca ttcccctgac ctttggtctg
    73741 ccaccagatg ttattcatcc gctattgttc tcttttgact tacaaggatg aacatatgct
    73801 aacagtaccg ggattatgct ggctatgccg aatgccactg gcgttaggtc attgggggat
    73861 ttgttcggtc tgttcacgcg ccacccgcac agataaaacg ttatgcccac aatgtggatt
    73921 acccgccaca cactcccatc ttccctgcgg tcgctgcctg caaaaaccgc cgccctggca
    73981 aagactggtc acggttgccg actatgcgcc gccgttaagt ccgcttatcc accagcttaa
    74041 attttcccgg cgcagtgaaa tcgccagcgc cctgtcacgt ctgttactat tggaagtctt
    74101 acacgctcgt cgcaccaccg ggttgcaatt gccggatcgc atcgtcagcg ttccgttatg
    74161 gcagcggcgt cactggcgtc ggggatttaa tcagagcgat ttgctgtgtc agccgttatc
    74221 acgctggttg cactgccaat gggatagcga agccgtcaca cgtacacggg ccactgcgac
    74281 ccagcatttt ctcagtgccc ggctgcgcaa gcgcaacctg aaaaatgcct ttcgtcttga
    74341 attgcccgtg caaggtcgcc atatggtgat tgtggatgat gtcgttacca ccggaagtac
    74401 cgtcgcagag attgcgcagt tgcttttacg caatggtgcg gcggctgtcc aggtctggtg
    74461 cctttgtcga accttgtaga gcctcgatga tgggcgtatt ataaccaact aaaatagtca
    74521 actattaggc cattactatg atccgtattt ccgatgctgc acaagcgcac tttgccaaac
    74581 tgctggcaaa tcaggaagaa gggacacaaa tccgcgtatt tgtgattaac cctggcacgc
    74641 ctaacgctga atgtggcgtt tcttattgtc cgccggacgc tgtggaagcc accgacacag
    74701 ccctgaaatt tgacctgctg accgcgtatg ttgatgagtt aagcgcacca tacctggaag
    74761 atgcagagat cgactttgtt accgaccagt tgggttctca gttaacgctg aaagccccga
    74821 acgccaaaat gcgtaaagtg gcagacgatg caccgctgat ggagcgcgtg gagtatatgc
    74881 tgcagtcgca gatcaaccca cagcttgctg gtcacggtgg tcgcgtttcg ctgatggaaa
    74941 tcaccgaaga cggttacgcc attctgcaat ttggcggcgg ctgtaacggt tgttccatgg
    75001 tcgatgtgac gctgaaagaa gggatcgaga agcagctgct gaacgaattc ccggagctga
    75061 aaggtgtgcg cgatctcacc gaacaccagc gcggcgaaca ctcctactac taagttatcc
    75121 cctcttttgt agattgcccg atgcgacgct aaagcgtcat ttcgggccta cgttgaacat
    75181 gtgccagacg ttggcgcagc attgctttgg caaatccctc ccttcgtttc tccgcgataa
    75241 tatgaccaac ctctcataat ttaaatttac cccgctctgg tgattctcaa acgccagatg
    75301 ttacccgtat cattcacatg ggtaccaaac atactcctga catctgacta caataattag
    75361 ttttagtggg tatcagtcgt ggtgccgcaa tatctctgtt cccgattggg ataattagag
    75421 tttgtcgtca gaaaattgac gttacccata acaaatgaaa ggccaggtaa atcatgccat
    75481 tagtcattgt tgctatcggt gtaatcttgt tgttgctcct gatgatccgc ttcaaaatga
    75541 acggcttcat cgctctcgtc ctcgtggcgc ttgctgttgg attaatgcaa ggaatgccgc
    75601 tggataaagt tattggctcc atcaaagccg gtgtcggcgg gacgctcggt agccttgccc
    75661 tgatcatggg ttttggcgca atgctgggca aaatgctggc agactgcggt ggcgcacaac
    75721 gtatcgccac cacgctgatt gccaaatttg gtaaaaagca catccagtgg gcggtggtgt
    75781 taaccggttt taccgttggt tttgccctgt tctatgaagt gggcttcgtg ctgatgctgc
    75841 ctctggtgtt taccatcgcg gcttccgcga atattccact gctgtatgtt ggtgtaccaa
    75901 tggcggctgc actgtctgtg acccacggct tcctgccacc gcatccgggc ccgactgcga
    75961 ttgccaccat tttcaatgcc gatatgggta aaaccctgct gtacggtact attctggcaa
    76021 tcccgaccgt gattctcgcc ggtccggttt acgctcgcgt gttgaaaggt atcgataagc
    76081 caatcccgga aggtctctac agcgcgaaaa ccttcagcga agaagagatg ccgagctttg
    76141 gcgtcagcgt ctggacttct ctggtgccgg ttgtgctgat ggcgatgcgt gcgattgccg
    76201 aaatgatcct gccgaaaggt cacgctttcc tgccggtagc ggagttcctc ggtgacccgg
    76261 taatggcaac gctgattgcc gtgctgattg cgatgttcac ctttggtctg aaccgtggtc
    76321 gttcaatgga tcagattaac gacacgctgg tttcttccat caaaatcatt gcgatgatgc
    76381 tgttgatcat cggtggtggc ggtgcgttca agcaggtgct ggtagacagc ggcgtggaca
    76441 aatacattgc ttccatgatg cacgaaacca acatttctcc gctgctgatg gcctggtcga
    76501 ttgctgccgt actgcgtatc gcgctgggtt ccgcaaccgt tgcggcaatc actgcgggtg
    76561 gtatcgcggc accgctgatt gcaacgacgg gtgttagccc agagctgatg gttattgcgg
    76621 tgggttccgg tagtgtgatt ttctctcatg tgaacgatcc gggcttctgg ctgttcaaag
    76681 agtactttaa cctgactatc ggcgagacca tcaaatcctg gtcgatgctg gaaaccatta
    76741 tctcggtgtg cggtctggta ggctgtctgc tgctgaatat ggtgatttga taacactgac
    76801 tgccggatgc atcgcatccg gcatcaaccg cactctactt cttcttcgct gcagctctgc
    76861 gccgtctgtc caaatccttc agcaacttgt tcacgccatc atcggcaaac atcgtctcaa
    76921 gcgttgcgga aagcttgcgt cgccagtttt tatactggta actggtgcca ggaatattca
    76981 ccggttcggc catatccagc cagtcttccg gctgtagtcc taacagagca ctgttactgt
    77041 cggcaatgta gcgctgcaaa ccacggttca gcgtcggcgt catcgacatc aacgatgcct
    77101 tatgcccggc acgttttggc agacaaccat atttatgcag tgcatccagc agcccttgct
    77161 tcgccagttc gcgatcctga tacagaccgc gcagtaccac ttcatccgga tacagcccca
    77221 gggttttgcc cagcgttaga tccccgctct cccagtaacc gcgtagcgta ggcaggtcat
    77281 gtgtcgccgc aaccgccatc gactgctccg gatacgcttt cggtgcacgg aacgtcttct
    77341 cgtggtcgtt ttcgaaatag agcactttgt aggagtacac accgctgctg cgcagcttac
    77401 cgacaatctc taccggtacg gtaccgagat cttcaccaat caccatacag cgatgacgtt
    77461 tactttcgag tgccagaatc gagagcagat catccaccgg atagtgaaca tacgcgccct
    77521 gatctgccgt ctcgccatac ggtatccacc acaaacgcag catcgacatc acatggtcaa
    77581 ttcgtaatgc gccgcagttt tgcatattgg cacgcaacag ctcgataaac ggttcatagg
    77641 cacgcgcggt gatgatatgc gggtccattg gcggtaatcc ccagttctgc cccaacgggc
    77701 cgaggatatc cggcggcgcg ccaaccgacg ctttcaggca atatagttca cggtcacacc
    77761 aggtttccgc cccaccttcc gctacgccaa ccgccagatc acgatacaag ccaatcggca
    77821 tttcatagcc ctggcttatc tcccagcagg cggcaaactg gctgtaagcc agccactgca
    77881 accagagata aaaatcgacg tcattacgat gttcttcgca gaactgacgc acttctggtg
    77941 aatccacgtt ctgatacatc tctggccatg caggccagcc ccagcgcatt tcgtcctctt
    78001 tcacttgctg ggcatgtagc gcatcaaagg ctgcctgcca gaacaggctg tcgccctgct
    78061 ctgcaacaaa ctggcgaaac gcggtcatct gctcatcatc acgttgcgcg aaacctttcc
    78121 acgccattcg taatgctgtc atttttaggg cggtaaccgt ggagtaatcg acccagtcgg
    78181 catcgcgcgc ctgttgcagc gtctgttgcg tggtcggcaa ctgccaccag gcctgagcct
    78241 cttcgctaag atggaaatct tcaacggcgt taacgtcgat ataaatcaca ttcagccaac
    78301 ggcgagaaga cgggctgtat gggctggcac tctccggatt tgccggatag agcgcatgaa
    78361 tcgggttcag gccgatgaac gacccgccac gttttgccac atccaccagc atcgctttga
    78421 gatcgccaaa atccccaata ccccagtttt tttccgatcg cagcgtataa agctgaacgc
    78481 aggcacccca cagcttttgt ttattcagca acgcctgcgg ttcgtaacag cgtttcgggg
    78541 cgacaatcac ccggcaatgc gcgcgctggt cgtcctgggt gagtgtcagc gtgtgataac
    78601 cttccggcag cttcgtcggt agattgaacg ctttgccccc cgttacatgg cctttgtact
    78661 gtgttccttc ttcggtggtc agcagccagc tatattcgcc gctgccctcc accaccatcg
    78721 gcattttttt gccgctggta taaaccatga cattcgggac tggcgttacc gccactttcg
    78781 tggcggtacg ttgatgcatc gcgtcaagca aacgccgttt ggtttcggcg ctaatcgact
    78841 gcggtttacc gtgggcattg atgtaattgg ggctaatccc cgccgccagc gcggcattat
    78901 ccagacgttt gctttccatc gagcttcctt agcgttttgc ctgccagata cgagcctgat
    78961 aatcgcgaat agagcgatcc gagctaaaca taccgcagcg ggcggtattg aggatcgccg
    79021 cgcgagtcca ggcctcctgg tcgcggtaca gcacatccac ctgcttttgt gcctcaacat
    79081 aggctgcgaa atccgccatc accagatacg gatcgccgcc ttgtttgccg atactgtgca
    79141 gcatctggtc gaaggcatgc ttatcgccgt cgctgtattt accgctttcc agctctttca
    79201 atactgcgtc cagcacctta tctttcttcc gccatttcac cgggtcgtag cctttggcca
    79261 gaatagcctt cacttgttcc acggtatgac caaaaataaa gatattttct tcaccgactt
    79321 tctcggcgat ttcaacgttc gcaccatcca gcgtaccgac agtaagcgca ccattgagcg
    79381 ccagtttcat attgccggta ccggaagctt ctttacctgc agtcgaaatt tgttcggaga
    79441 tatccgccgc cgggatcagt ttttccgccg ccgaaacgca ataatccggc aggaacacca
    79501 ccttcaactt atcgccaacc agtggatcgt tgttgatcac gtcagccact ttgttgatcg
    79561 caaagataat attcttcgcc aggtagtagc ccggtgccgc tttcgcgccg aagaggaaga
    79621 cgcgcggtac gcgatcagcc tgcgggtttt cacgaatttc tttgtacaac gccagaatat
    79681 gcagcagatt caggtgctgg cgtttgtact catgcagacg tttgatctga atatcgaaaa
    79741 tcgcctgtgg attgatctca ataccggtac gaactttcac aaactccgcc agacggactt
    79801 tattcgcctg cttgatctcg cgatattgct gacggaattt cgcatcatca gcgaattttt
    79861 ccagattgat cagctgatcg agatcgttag cccactcttt ttgcagtgat ttatccaaca
    79921 gagccgccag tgcagggttg cactgtttga tccagcgacg tggggtaata ccgttggtga
    79981 cgttatggaa tttgttcggc catagctggt gatattccgg gaacagatct ttcaccacca
    80041 gatccgagtg cagcgccgca acaccgttca ccgcgaaacc gccaaccaca cacaggttcg
    80101 ccatatgcac ttgtttatcg tgcaccaccg ccagtttggc ccacactttt tcatcgcccg
    80161 gccaggtttt ctccaccagc gttttaaagc gagtattaat ttcgttaata atctgcatat
    80221 ggcgcggcag taagcctttc accagtttca catcccagcg ttccagcgct tctggcatca
    80281 gggtatggtt ggtgtaagcg aaagttttgc tggtaatggc ccaggcgtca tcccagctca
    80341 tctggtgctc atcgatcagc acgcgcagca gttctggaat cgcgatagtt gggtgggtat
    80401 cgttcagctg aataacttcg taatccgcca gttcgtgcag tttacgcccc gccagatgat
    80461 ggcgacgcaa aatatccgct accgaacagg cacactggaa gtactgctgc atcaagcgca
    80521 gctttttacc ggcagtatgg ttgtcgtttg gatagagaac tttggtcagt ttttccgcat
    80581 tgatgccctg ctgttcggca cgcaggaaat caccgtcgtt aaatttagtc agatcaaacg
    80641 gatgcgcgtg cgtcgcctgc cacagacgca gcggctgcgc cacgccatta cgatagccga
    80701 caacggggag atcccacgct tgaccggtaa tggtaaactc cggctcccag cgtccgtctt
    80761 tcgtcacttt accgccaatc cctacctgca catccagtgc ttcgttgtgg cggaaccacg
    80821 ggtagttact gcgatgccag tcatccggcg cttcaacctg tttgccatcg acaaaagact
    80881 ggcggaacaa accatattga tagttcagac cgtaacccgt cgcagactga ccgacagttg
    80941 ccattgagtc gaggaagcac gccgccagac gtcccagacc accgttaccc agcgccgggt
    81001 cgatctcttc ttccagcagg tccgtcagat tgatgtcata agccttcaac gaatcctgta
    81061 catcctgata ccagccgaga ttcaacaggt tgttgcccgt caggcgacca atcaaaaact
    81121 ccattgagat gtagttaaca tgtcgctgat tcgccaccgg cttggcgaat ggctgagcac
    81181 gcagcatttc ggccagtgct tcactcactg ctagccacca ctggcgagga gtcatttcag
    81241 ccgcagaatt taagccataa cgctgccact gacgtgaaag cgcttcctga aattgcttat
    81301 cgttaaaaat aggttgtgac ataggagttc cacttttctt agattttcaa cacaacgtta
    81361 tcgctagttt gccaggctcg atgttgacct tcctcatcct gcgggggatt aggcagggag
    81421 gagttgcggg gatgagcaag gaaatgtgat ctcaaccact taaagctagt gcaaaccaca
    81481 ggattagcag caaatcaatg caatacagcg cagaaaatct gtatctaagt gcaaaaaatg
    81541 gccgttgcgt attttcaaaa agcggaaggt aactctataa attaagtaaa ggagtgaaac
    81601 agtttcataa gtaaaatatc cagtgtgctc catctcattc ttaatagatt tattaagatc
    81661 atctttttag atggcacttt catcaggaat gaagaagaaa cccttgctta aatgaatctg
    81721 atgaacataa gggaaaccag tattcacgct ggatcagcgt cgttttaggt gagttgttaa
    81781 taaagatttg gaattgtgac acagtgcaaa ttcagacaca taaaaaaacg tcatcgcttg
    81841 cattagaaag gtttctggcc gaccttataa ccattaatta cgaagcgcaa aaaaaataat
    81901 atttcctcat tttccacagt gaagtgatta actatgctga ttccgtcaaa actaagtcgt
    81961 ccggttcgac tcgaccatac cgtggttcgt gagcgcctgc tggctaaact ttccggcgcg
    82021 aacaacttcc ggctggcgct gatcacgagt cctgcgggct acggaaagac caccctcatt
    82081 tcccagtggg cggcaggcaa aaacgatatc ggctggtact cgctggatga aggtgataac
    82141 cagcaagagc gtttcgccag ctatctcatt gccgccgtgc agcaggcaac caacggtcac
    82201 tgtgcgatat gtgagacgat ggcgcaaaaa cggcaatatg ccagcctgac gtcactcttc
    82261 gcccagcttt tcattgagct ggcggaatgg catagcccac tttatctggt catcgatgac
    82321 tatcatctga tcactaatcc agtgatccac gagtcaatgc gcttctttat tcgccatcaa
    82381 ccagaaaatc tcaccctggt ggtgttgtca cgcaaccttc cgcaactggg cattgccaat
    82441 ctgcgtgttc gtgaccaact gctggaaatt ggcagtcagc aactggcatt tacccatcag
    82501 gaagcgaagc agttttttga ttgccgtctg tcatcgccga ttgaagccgc agaaagcagt
    82561 cggatttgcg atgacgtttc cggttgggcg acggcactac agctaatcgc cctctccgcc
    82621 cggcagaata cccactcagc ccataagtcg gcacgccgcc tggcgggaat caatgccagc
    82681 catctttcgg attatctggt cgatgaggtt ttggataacg tcgatctcgc aacgcgccat
    82741 tttctgttga aaagcgccat tttgcgctca atgaacgatg ccctcatcac ccgtgtgacc
    82801 ggcgaagaaa acgggcaaat gcgtctcgaa gagattgagc gtcaggggct gtttttacag
    82861 cggatggatg ataccggcga gtggttctgc tatcacccgc tgtttggcaa cttcctgcgc
    82921 cagcgctgcc agtgggaact ggcggcggag ctgccggaaa tccaccgtgc cgccgcagaa
    82981 agctggatgg cccagggatt tcccagcgaa gcgattcatc atgcgctggc ggcaggcgat
    83041 gcgctgatgc tgcgcgatat tctgcttaat cacgcctgga gtttgttcaa ccatagcgaa
    83101 ctgtcgctgc tggaagagtc gcttaaggcc ctgccgtggg acagtttgct ggaaaatccg
    83161 cagttagtgt tattgcaggc gtggctaatg caaagccaac atcgctatgg cgaagttaac
    83221 accctgctag cccgtgctga acatgaaatc aaggacatca gagaaggcac catgcacgca
    83281 gaatttaacg ctctgcgcgc ccaggtggcg attaacgatg gtaatccgga tgaagcggaa
    83341 cggctggcaa aactggcact ggaagagctg ccgccgggct ggttctatag ccgcattgtg
    83401 gcaacctcgg tgctgggtga agtgctgcac tgcaaaggcg aattgacccg ctcactggcg
    83461 ctaatgcagc aaaccgaaca gatggcacgc cagcacgacg tctggcacta cgccttatgg
    83521 agtttaatcc agcaaagtga aattctgttt gcccaagggt tcctgcaaac cgcgtgggaa
    83581 acacaggaaa aagcgttcca gctgatcaac gagcaacatc tggaacagct gccgatgcat
    83641 gagtttctgg tgcgcatccg agcacaactg ttatgggcct gggcacggct ggatgaagcc
    83701 gaagcgtcgg cgcgtagcgg gatggaagtc ttgtcgtctt atcagccaca gcaacagctt
    83761 cagtgcctgg caatgttgat tcaatgctcg ctggcccgtg gtgatttaga taacgcccgt
    83821 agccagctga accgtctgga aaacctgctg gggaatggca aatatcacag cgactggatc
    83881 tctaacgcca acaaagtccg ggtgatttac tggcaaatga ccggcgataa agccgccgct
    83941 gccaactggt tgcgtcatac ggctaaaccg gagtttgcga acaaccactt cctgcaaggt
    84001 caatggcgca acattgcccg cgcacaaatc ttgctgggcg agtttgaacc tgcggaaatt
    84061 gttctcgaag aactcaatga aaatgcccgc agtctgcggt tgatgagcga tctcaaccgt
    84121 aacctgttgc tgcttaatca actgtactgg caggccggac gtaaaagtga cgcccagcgc
    84181 gtgttgctgg acgcattaaa actggcgaat cgcaccggat ttatcagcca ttttgtcatc
    84241 gaaggcgaag cgatggcgca acaactgcgc cagctgattc aactcaatac gctgccggaa
    84301 ctggaacagc atcgcgcaca gcgtattctg cgagaaatca atcaacatca tcggcataaa
    84361 ttcgcccatt tcgatgagaa tttcgttgaa cgtctgctga atcatccgga agtgccagaa
    84421 ctgatccgca ccagcccgtt gacccaacgt gaatggcagg tcctggggct gatttactcc
    84481 ggttacagca atgagcaaat agcgggtgaa ctggaagtgg cggcaaccac catcaagacg
    84541 catatccgca atctgtacca aaaactcggc gtcgcccacc gtcaggctgc cgtgcagcac
    84601 gcgcagaaac tgctgaagat gatgggttat ggggtgtgag tttagccgga taatgcattg
    84661 gatccggttt acatctttac atcattcaat actcacccgc gttacgccat ctgtttctat
    84721 caaaccaaac cgcaccggca agaaacgctc caccaccgcg atattggtca gcagatggca
    84781 tgaaggatgg gcgaccttaa attctcccgc gcccgccagc gccatcggca gtaccagttg
    84841 gtcggcgaga tattccccca ccgccgctgg gcttgccagg tagcgtttca cctctttcac
    84901 taactgcgcg gctaccacct cggcactgac gcgcttttca ccgacgacga aaaagcgttc
    84961 agtgatattt tcactttcga cttccagcga gacggtatta cccggcccct ggtcgcgcgg
    85021 caggttatga atattctgtt catgcaggga aaaactcccc gccagtgtag cgatttcacg
    85081 ctcagcaaca tggcgcggca caccagctaa tagaacttct ccacgcatct gcacaatgtt
    85141 cccgcgctcg ccaagttgca aggtgttaaa cgatgccacg ggcgagactt ccgttgccac
    85201 cacgccgcct ccggcaggat aaaaaccgtg acgtaacagc gtggtttgct gatgaattcc
    85261 tattttcgcc agcagcggct ccagcacccg gcggataaaa tccgcaggcg gggccgacgg
    85321 gttatcggtg cctccgctca cttcaacacg cgaaggtcca tcggcaaacc acagcgcggg
    85381 cagcaccgtt tgcagcacca gcgtacagct tccggcacta ccgatagcaa agcggtactc
    85441 gccgccgcgc acggtgccag gccggaagac cagacgctgc gaccccagct ccgcgccttc
    85501 caccgttgcc ctacaaattt ccgcagccgc ttttaccgcg gtcagatgct ggcgcaacag
    85561 ccccggtttc gcccgcccgg cacgaatgct ggtgatggta aatggctggc cggttatcat
    85621 cgacaggctc agcgccgagc gcaggatctg cccgccacct tcgccctgtg cgccatccag
    85681 cgcaatcatc cttttcatca tttatccttt tacgcacacc acctgacgca gggtatagat
    85741 aacttccacc agatcgcttt gtgccgccat caccgcatca atatctttat acgccatcgg
    85801 gatttcgtcg atcacttcgg catctttacg gcattccaca tgcgcggtgg cacgaatttg
    85861 atcttccacg ctgaacagtt ttttcgcttt agtacggctc attacccgcc cggcaccgtg
    85921 gctgcacgaa cagaacgact cttcatttcc cagcccacgg acgataaagc tttttgctcc
    85981 catcgaaccg ggaataattc catattgacc agcacgcgca gacaccgcgc ctttacgcgt
    86041 cacgtagatc tcttcaccaa agtgctgttc tttttgcaca tagttgtggt gacagttgat
    86101 ctcttccatc gccagcgttt gtggctgtct gaccgttttc tgcgtaatgc tctgcaacgc
    86161 cgttaccacg ttttccatca tcgcatcgcg gttaaggctg gcgaaaagct gcgcccaggc
    86221 cacggctctt aggtaatcat caaagtattc cgtaccttcc ataaagtacg ccaggtcgcg
    86281 cgatggcaac gtttcaagct ggtcctgcat ctctttttgt gccaaatcga taaagtaagt
    86341 cccgatggcg ttaccaattc cgcgtgaacc ggagtgcagc ataatccaca cctggtctga
    86401 ctcatcaagg cagatttcaa taaagtggtt acccgttccc agcgttccca ggtgtttata
    86461 gttgttagtg ttaaggaaac gcggatattt ttgcgttaac cactgataac cggcttcaag
    86521 ctcagcccat ttcgcatcga cgttaacagg cgggttttcc catgcgcctt tatcacgttt
    86581 acaacggcca gtggtacgcc cgtgcggcac ggccatttca atcgcctgac gcagctctgc
    86641 caggttttca ggcaggtctt ccgccgttaa cgcggtacgc agcgcgttca ttccacagcc
    86701 aatatccacg cccaccgccg ccggaataat cgcccctttg gtcgggatca cgctaccaat
    86761 ggtggaacct ttacccaggt gtacatcagg cattaccgca atatgtttga aaataaacgg
    86821 catcttcgcc gtattaataa gttgctgacg cgcatcggct tctaccggca cgcctttggt
    86881 ccacattttt accggggcat tttcagtggt cagtaattcg taattcattt tgtttttctc
    86941 ttttcgttgt ttgctgtcgt gataattgca accgtcgtgc cagaaaatta aataagcaga
    87001 ttaatttttt aattcattgt ttttaaatag gttaaatctt tacgtccgta aacggagatt
    87061 ttccacaaaa ccaattcacc gataatgaaa tatcgtcttt tataaggata tctaagatgc
    87121 gtaaaacagt ggcttttggc tttgtcggta ccgtgctgga ttatgccgga cgcggcagtc
    87181 agcgctggtc gaaatggcgt ccgacgcttt gtttatgcca gcaagaatcg ttagtcatcg
    87241 atcggctgga attgttgcac gacacccgct cgcgctcgct attcgaaacg cttaaacgcg
    87301 atatcgccag cgtttcgcca gaaacagaag tcgtgggcgt tgagattgaa ctgcataacc
    87361 cgtgggattt cgaagaggtt tacgcctgcc tgcatgattt cgcccgtggt tacgagtttc
    87421 agccagaaaa agaagactat ttaattcaca tcaccaccgg cacccacgtc gcgcagattt
    87481 gctggtttct gctggcggaa gcgcgttacc tgcccgcccg gctgatacaa tcttcacctc
    87541 cgcgcaaaaa agaacagccg cgcggcgcag gtgaagtgac gattatcgat ctcgatttaa
    87601 gccgttataa cgccatcgcc agccgctttg ccgaggaacg ccagcaaacg cttgattttc
    87661 ttaagtccgg cattgccacg cgtaaccccc acttcaaccg catgattgag cagatcgaaa
    87721 aagtggcgat caaatcccgc gcgccgattc tgcttaacgg tccaaccggc gcgggcaagt
    87781 catttctggc gcgacgcatc ttcgagttaa aacaggcgcg gcatcagttt agcggcgcgt
    87841 ttgtggaagt gaactgcgcc accctgcgcg gcgataccgc catgtcgacg ctgtttggtc
    87901 atgtaaaagg cgcgtttacc ggggcgcggg aatctcgtga aggtttatta cgcagcgcca
    87961 acggcggaat gttgtttctt gatgagattg gcgaactggg cgcagacgaa caagcaatgc
    88021 tgctaaaagc cattgaagag aaaacctttt acccgtttgg cagcgatcgc caggtgagca
    88081 gtgattttca gcttatcgcc ggaacggtgc gcgatttgcg ccagctggtt gccgaaggca
    88141 aatttcgcga agatctgtac gcgcggatca atctctggac cttcaccctg ccgggtctac
    88201 gccagcgcca ggaagatatt gaaccgaacc ttgattatga agtggagcgc cacgccacac
    88261 tcactggcga cagcgtgcgt tttaacaccg aagcgcggcg cgcctggttg gcttttgcga
    88321 cctctcccca ggcaacatgg cgcggtaact ttcgcgaact ttctgccagc gtcacgcgga
    88381 tggccacctt tgccactagc ggacgcatca ctctggacgt ggttgaagat gagataaacc
    88441 gtctgcgcta taactggcag gagagccgcc cctccgcgct tacggcgttg ctgggcgcag
    88501 aggcagaaaa catcgacctt tttgacaggc tgcaactgga acacgtgata gctctctgtc
    88561 gccaggcaaa gtcgctttcc gctgccggac gcctgctttt tgatgtttcg cgccagggca
    88621 aagccagcgt caatgacgcg gatcggctac gcaaatacct ggcgcgtttt ggtctgacgt
    88681 gggaagccgt gcaggatcag cacagctcca gttgaatatg atggtccgtc agcacctgca
    88741 ttacgcttac tggcggcggg gcgtcggtgt agacggcatc taccatgctg atgctgccca
    88801 tattgaccat cgcgttacgg ccaaatttcg agtgatcgac aaccagcata acgtggcgcg
    88861 agttctcaat aatggcgcgc ttggtgcgaa cttcgtggta atcgaactcc agcagcgagc
    88921 cgtcgctatc gatgccgctt atccccagaa tgccgaaatc aaggcggaac tgggagataa
    88981 aatcgagcgt cgcttcgcca atgatcccgc catcgcggct gcgtaattcg ccaccggcga
    89041 gaataatgcg gaaatcttct tttaccatca acgtgttagc aacattgaga ttgttggtga
    89101 caatacgcaa attgctgtga ttaagcagcg cgtgcgccac cgcttccggc gtggtgccga
    89161 tatcgataaa cagtgtcgag ccgttgggga tttgctccgc cactttgcgg gcaatcctct
    89221 ctttttcttc ggtctgggtg gctttgcgat cgtgccacgg cgtgttaacc gaactggaag
    89281 gcagcgccgc accgccatgg tgacgcagga tcaggttttg ctccgccagc tcattgaggt
    89341 cgcggcgaat agtctgcggg ctgacggaga aatgctctac cagctcttca gtactgacat
    89401 aaccctgctg tttaaccagt tcgataatac cgttgtgacg ttgtgtttgt ttcatttata
    89461 aatccctgga attattttcg ttttcgcgca ttgagcgaat caacaaaagc catcgctaaa
    89521 cccacggcta acccggcgat gtgtgctccg ttcgccatcg acatcccaaa caaatcaaac
    89581 catccggcga caatccagat cagcgcaaag ataattaacc cacgttgcag gtaaatgcca
    89641 ctttgcggat cgcgttcacc acgtagccag acgtagccca tcagcgcata caccacgcca
    89701 gaaagcccgc caaaccacgg cccgctgaat ttttgctgca catagccgct taacagggcg
    89761 ctaatgagag taataacaat tagcttaccg ctaccgaggc gtttttccac cgcaccgccg
    89821 agataccacc accagagcag gttaaagagg atatgcatca gcgagaagtg cattaacgcg
    89881 tgggtgaagt aacgccagaa ctcaaatttc agtgttggat cgaatggcca ggccagccat
    89941 aacatcactt cctgatcgcc gagaatttgc atggcgataa acaccaccac gcaggcgatc
    90001 atcaccaccc aggttaccgg acctgcgcgt tcacgcaagg cggcaaagaa aggataacgg
    90061 cgataatgca ggccactgcc ggtatggcct gcctgccagc tggccgccag ataacgcgga
    90121 tctgccgggt tttcgagaaa acgcgccagc tccgcccgta cgcgctcggc ctgggactca
    90181 tccgccagcc agacatcgct ttggttatgt tgttgaatcg tgaggataac accctgcgtc
    90241 gccatgtaat caacaaacgc ctgcgccacg cgggggttag caaaagaggt aatcatcaac
    90301 atcgttgctg tcgcttattc cacacaaaag gggacagtat aaagcgttac gcgccgtacg
    90361 ccacctctgc gggaaactga cgttgccagg cttcaaagcc gccgtcaatg ctatagacca
    90421 catcgtagcc ctgttgcagc agatactgcg ccgcgccttt gctgctattg ccgtgataac
    90481 acatcaccat caccggagtg tcaaagtcgt tatcacgcat aaacgcgccc agcgtgtcgt
    90541 tggttaaatg gaaagcctgc accgcatgcc ccatagcgaa actctgtgga tcgcgaatat
    90601 cgaccagcac cgcctctttt tcctgcaact tctggtgcgc gtcggcaacg ttaatacatt
    90661 cgaactgatc catgcgtctc tctttcttta caaacaagtg ggcaaattta ccgcacagtt
    90721 tacgtcgaag cggcagataa acgccataat gttatacata tcactctaaa atgttttttc
    90781 aatgttacct aaagcgcgat tctttgctaa tatgttcgat aacgaacatt tatgagcttt
    90841 aacgaaagtg aatgagggca gcatggaaac caaagatctg attgtgatag gtggcggcat
    90901 caatggtgct ggtatcgcgg cagacgccgc tggacgcggt ttatccgtgc tgatgctgga
    90961 ggcgcaggat ctcgcttgcg cgacctcttc cgccagttca aaactcattc acggtggcct
    91021 gcgctacctt gagcactatg aattccgcct ggtcagcgag gcgctggctg aacgtgaagt
    91081 gctgctgaaa atggctccgc atatcgcctt cccgatgcgt tttcgcctgc cacatcgtcc
    91141 gcatctgcgc ccggcgtgga tgattcggat tggtctgttt atgtacgatc atctgggtaa
    91201 acgcaccagc ctgccgggat caaccggttt gcgttttggc gcaaactcag tgttaaaacc
    91261 ggaaattaag cgcggattcg aatattctga ctgttgggta gacgacgccc gtctggtact
    91321 cgccaacgcc cagatggtgg tgcgtaaagg cggcgaagtg cttactcgga ctcgcgccac
    91381 ctctgctcgc cgcgaaaacg gcctgtggat tgtggaagcg gaagatatcg ataccggcaa
    91441 aaaatatagc tggcaagcgc gcggcctggt taacgccacc ggcccgtggg tgaaacagtt
    91501 cttcgacgag gggatgcatc tgccttcgcc ttatggcatt cgcctgatca aaggcagcca
    91561 tattgtggtg ccgcgtgtgc atacccagaa gcaggcctac attctgcaaa acgaagataa
    91621 acgtattgtg ttcgtgatcc cgtggatgga cgaattttcc atcatcggca ccaccgacgt
    91681 agagtataaa ggcgatccga aagcggtgaa gattgaagag agtgaaatca actatctgct
    91741 gaaagtgtat aacgcgcact ttaaaaaaca gcttagccgt gacgatatcg tctggaccta
    91801 ctccggtgtg cgtccgctgt gtgatgatga gtccgactcg ccgcaggcta ttacccgtga
    91861 ttacaccctt gatatccatg atgaaaacgg caaagcaccg ctgctgtcgg tattcggcgg
    91921 taagttgacc acctaccgca aactggcgga acatgcgctg gaaaaactga cgccgtatta
    91981 tcagggcatt ggcccggcgt ggacgaaaga gagcgtgctg ccgggtggtg ccattgaagg
    92041 cgaccgcgac gattatgccg ctcgcctgcg ccgccgctat ccgttcctga ctgaatcgct
    92101 ggcgcgtcat tacgctcgca cttacggcag caacagcgag ctgctgctcg gcaatgcggg
    92161 aacggtaagc gatctcgggg aagatttcgg tcatgagttc tacgaagcgg agctgaaata
    92221 cctggtggat cacgaatggg tccgccgcgc cgacgacgcc ctgtggcgtc gcacaaaaca
    92281 aggcatgtgg ctaaatgcgg atcaacaatc tcgtgtgagt cagtggctgg tggagtatac
    92341 gcagcagaag ttgtcgctgg cgtcgtaagg atgatgcccg gttgtgaagt gcaaccgggc
    92401 tatttggatt ccttagacta ttcattgtaa ataattgaat aacggcccct gagctaaaat
    92461 cgccaaactt aagcacgtta cccaatcgcc ccacttcttc ctctggtttc tattttttct
    92521 gcaccaatgg caataatatc ccaatgcaaa accaagctgt gcactaatcg ggccgacaaa
    92581 atcagtccaa ggctgcggcc aaaaagggga aaaattcagg ggaagccaga caaacgtcag
    92641 aaagaaattt cggatatttt ccagcaacgc tgatttctct ggagaaatat aaagaatgaa
    92701 attcgagaga attaaccaga aatagaccgg cgaaaagacc agccagcaat gcagtctgtt
    92761 ttttaattca ggaggcgttc tgcgggagaa ttgccaaccg atgccagcca ttacactgaa
    92821 aaaaagtgca tattcaacca ttaactcaag ttgtagttcc tgaagagaaa tacgcacaac
    92881 caggagcgga aaggagaata ttatttctgc cagaaaagtt aacgtgaata taagtacggc
    92941 taagacaata accgtcggcc ataccaataa tggcacgata acaaaaccga gaattacagg
    93001 gggaatataa agccggaatt taatcatttt tattttcctg gtgattcgga tgatgcgtca
    93061 tacatcgcgc ataaacactc atcaattatc cagtcgtcta cccagacctg atgctgtgtg
    93121 ccctgttctg gctgctttgg tgggttctca atcgcttgcc gcgctctttc tttcagtaaa
    93181 ggatgattat ttctagccca ggcattgatt tgggtggtaa tggcgttatc gatatcgtcc
    93241 tggttatctc tgatataatc ctcacaatat ctgttcaata aacaggcaag agaaacggtc
    93301 gaccaaaagc cgggaactga attaatggca tccagcaatg gcgcaagagc gatatcggca
    93361 cggaaaaaca tttccagcat atactgtaat tcgacagctt catcgccgga tacccaatca
    93421 gtcatatact ggcaggcaaa tcgctgtaaa attgcccgtt catccgcacg ccagttttct
    93481 ttgttggcaa ggttgatacg gcgcagagaa aaaactacgt gaatattgtc aaaatgcccc
    93541 tgtgcaacaa attccagtaa acgctgcaga aaatagcgga cctcatcgct attttgcgga
    93601 tcaggccctg ggctactatt ccaggcattg attaaggaat acgggatcgc tcgtaacgat
    93661 gtactccgta atgccttttg ttcctgctga ctcaggcaat attcacaaca aaccacaaat
    93721 ttctgcggca cccgataacg acgaaaggtg tgataaatct gctcaattaa tgcctgcatg
    93781 gcggggctgg gttgcatagt taccccctcg cccgctgatc gcgtgtaaac ggttgataga
    93841 caaaacgagc attcgccgcg ttcaggcgaa taagggcgct acgcgaaagg gaaaagctgg
    93901 ggcgaatgca actattgcag tctcgacagc tttcctctgc gcaattttcc actgagtaat
    93961 gacgcgtgta gagtttcagt tcggcctgct tatcgcgaaa tccgcgtaaa ctgttattgt
    94021 tatgaaattt ccaagcatct ttaaagacct gaaagatata gacatcctca atccaggagt
    94081 cacatttttt aagcgggcgc tttgctatta tcacccgctc atccggattt agcatcgccg
    94141 taaattgtac ggtgacgtca tttgtcgtgt tcaggtcaca ttgaacatct ggtacatcac
    94201 tgatataggg gtcaaatcca aaatcagcac cacaggcaca aagacggcta atggtggcgg
    94261 tatcgaggga aaatcccggc cataccttgc tggaggttaa gccaatattt aatgtgacat
    94321 catatcgaag caatccctca agaccgtcag caccgacgaa cttttcccct tcatccaggg
    94381 cctggttaat aagttgctga acaatgcctt catcccagta tgccgatttg cagttggcaa
    94441 tcgtaatatc cacgtctgtg aagggatcag taccggcaag gcttgcaaat aatgtattaa
    94501 ctaattccat ttttattatc cagctttaag gtagcgtctt atacatcggg acataaccta
    94561 catcttccac caaactctgt ccctgcggcg ttaaaaacca ctccaacaat ttttgtgtct
    94621 ctgacgtcgt attttcccgc gttaccataa aggcatcgac gatataggga tatttgccgt
    94681 tgcgaatgtt ttcggcagtc ggtgcaatac cgttaatcgc tagcagtttt atatttttat
    94741 cgtcgttcat ttgtgttgcg taataacgga aggtgtaacc tattgcgttg tttgtattac
    94801 ggtattcggc aacgacttta atcattccct ccatcatact tgccacttct gtttcctgcg
    94861 gcgagatcat gcggacattt ttcatgacct gggattgcat caccgtctgg ctaccagagt
    94921 cttccgggcg ttgccaggtc tggatctcct gatcgttacc gccaaccgtg cgccagttgg
    94981 taattgcacc actgaagatg tcacgcactt gttgttcggt cagggaatta accgggttat
    95041 ctacattgac gatgaaaaca aacgcttcac gggcaaaagg cgtgtaaatc aaagtgacgc
    95101 ccgattcctc cgcgcgtttt ttctgcccac cggaaggttg agccacaaag ataatatcgg
    95161 cattaccttt aacaatcttg ttatatgctt cgggagtacg agagttcgcc agatattccc
    95221 attcgtgaaa atcttccggc aaaacgctta gtgcataaaa ggcagaggca taaatggggt
    95281 acgccgccgt tgcgccatca agtcgcggcc agttttgcgt gaactgaatt tgcggtttac
    95341 cacgcagtgg tgtcagttga ttattgagtt tatcgggtcg ccaggcccag gtatcaatat
    95401 tttcacgcac accgacgccg ggattaagct gtttatataa ataagcctgg taaccggcga
    95461 caaaaccaag catgagaagg ataaacaaga gagaattacg gcaacgtaac ccggacgtgc
    95521 ccgttaaagg atatctggtc ggccagccgt accccagcga aaaactgata tgcgcgccaa
    95581 caggtataat gactaaccac cagtactcac caccgactaa tgaggctaac aaaacgccca
    95641 gccaggggag cataagatca gccaaccaac ccgctcccat ctggcgaaat tgataatctg
    95701 ccagatgcag cgcgattatc cagaccccca gggttaataa tgcgggtact aaaaaaagac
    95761 attgtcgcca gacggctatt tctctttttt ctggtgcact atcgtccgcc cacactcgcc
    95821 ccgagaaaaa tagcattaat gcgcccagcg ccagcataaa aatgttgagc agaaggtaaa
    95881 ttgttggcgt gataaagata ttacaaactt caataatacc ggcaagtccg acacccagca
    95941 tggcaataac cgccgccaaa aactgcgcca gaatggggat gccgaaaaaa gtcattacca
    96001 gcgaggtcaa aatccatttt ctgttttgca ttattctttc cattctttct gaatggcgaa
    96061 attatactcc ccgagtcccc ttgccccttc tggacacttt tccgaaatga tggcgaaaaa
    96121 aacgggaccc tttggccccg ttctatttat tagtgaaatt acaatctcac cggatcaata
    96181 tgccagatat gatcggcgta ctctttgata gtacggtcag aagagaagta gcccatattg
    96241 gcaatgttca gcatcgcttt tgcggtccac tcttcctgaa gctcgtagag ttcgtcgact
    96301 ttatcctgac aatcgacata gctgcgataa tccgccagaa cctgatagtg atcgccgaag
    96361 ttgatcagcg aatcaaccag atcgcgatag cgacccggat cttccggact gaatacaccg
    96421 ctgccgattt gcgtcagcac ctgatgcagc tcctcatctt tctcgtagta ttcacgcggt
    96481 ttgtagccct gacgacgcag ttcttccact tcttccgcag tgttaccaaa gataaagata
    96541 ttgtcagcac cgacatgatc cagcatctcg acattcgcac cgtccaacgt accgatagta
    96601 agcgcaccgt taagcgcaaa cttcatgtta ctggtgccgg aagcttccgt ccccgccagc
    96661 gaaatctgtt cagacagatc ggccgccgga atgatcaact gcgccaggct aacgctgtag
    96721 ttcgggatga acacgacttt cagcttgtcg ccaatctgcg gatcgttgtt gatcactttc
    96781 gctacgtcat tgatcaaatg aataatgtgc ttcgccatgt aataggccga agccgcctta
    96841 ccgccaaaaa tattcacgcg cggtacccac ttcgcatccg ggtcggcctt gatgcggtta
    96901 tagcgggtaa tcacatgcaa cacattcatc aattgacgtt tgtattcgtg aatacgtttg
    96961 atttgtacat cgaacaacgc ctttggattc accaccacat tcagctgctg ggcgatatac
    97021 tctgccagac gctttttgtt ctccagcttc gcctgatgca cagcgtgatt aaccattggg
    97081 aaatcacagt gttgttgcag ctcattaagc aggctaaggt cggtgcgcca gttacggccc
    97141 aggtgttcgt ccagcacggc tgaaagcgat gggttcgcta ccgctagcca gcgacgcggc
    97201 gtcacaccgt tggtgacgtt ggtgaaacga cccgggaaga ttttcgcaaa gtcggcaaac
    97261 aacgattgca ccatcagatt agagtgcagt tccgatacac cgttaacttt gtggctcaca
    97321 acaaccgcca gccaggccat acgcacacga cgaccgttgg attcatcaat gatcgacgcc
    97381 cgtcccagca gatcggtatc gttcggatac tgttcctgca aggttttcag gaaatagtcg
    97441 ttgatttcaa agatgatctg caggtgacgc ggcagaattt tacccagcat atcaaccggc
    97501 caggtttcca gcgcctcgct catcagcgtg tggttagtgt aggagaagac ctgacaacac
    97561 acctcaaacg cgtcgtccca gctaaattgg tgctcatcga tcagcagacg catcatctca
    97621 ggaatcgaca gtaccggatg ggtatcattg agatgaatcg cgattttatc cgccaggtta
    97681 tcgtaggttt tatgcaactg ataatggcgg cttaaaatgt cctgaatggt cgaggaaacc
    97741 aggaagtatt cctgacgcag gcgcagctca cgcccggagt aggtggagtc atccggatac
    97801 agtacgcgag atacgttctc ggagtggttt ttatcttcca ctgccgcgaa gtagtcaccc
    97861 tggttgaatt taccgaggtt aatttcgcta ctggcttgcg cactccacaa acgcaacgtg
    97921 ttggtcgcgt cggtgtcgta accagggatt atctgatcgt aagcgactcc cagaatctct
    97981 tcggtttcaa tccaacgcgt ttttttacct tcctgctgaa tgcgaccacc aaaacggact
    98041 ttatagcgcg tgttgtggcg tttgaattcc cacgggttac cgtattccag ccagtagtct
    98101 ggcgactctt tctggctacc gttaacgatg ttctgtttga acataccgta gtcatagcgg
    98161 atgccgtaac cgcgccccgg caaccctaac gtcgccagag aatcaaggaa gcaagccgcc
    98221 agacgtccca ggccaccgtt accgaggcct gggtcatttt cttcatcaat cagctcttcg
    98281 agatttaacc ccatcgcttc cagtgcgccc tgtacatctt cgtaaattcc tagcgacaac
    98341 atggcgttgg agagcgtacg gccaatcaaa aactccatcg acaggtagta aacctgacga
    98401 gtttcttgcg acaactgggc acggtttgaa cgtaaccagc gctccacgag acgatcgcgc
    98461 acagcaaata acgttgcgtt cagccattca tgtttattgg cgacgaccgg gtcctttcca
    98521 atcgtaaaca tcagcttgta agcgatagag tgcttaagag cttctacgct aagcgtgggc
    98581 gatgaatatg taaacggagc attcatatag gcgtttcctg aaaactattt caagcgatag
    98641 taaagctcac ggtacgactt cgccgcgacc tgccagctaa aatccattgc catagcctga
    98701 cgttgcacaa accgccacag tgaaggacgg gaccacagta caaaagcacg tcgaatagcc
    98761 cgtaacagcg accaggcatt actatcttca aagacaaacc cactggcgac gccatctgca
    98821 aggttctcaa gagaacagtc agaaaccgta tcagcaagcc caccggtgcg ccgcactaac
    98881 ggcagcgtac cgtacttcaa tccataaagt tgcgttaagc cgcacggttc aaaacggctg
    98941 ggcaccagaa tgacgtccgc gccgcccata atgcgatgcg aaaatgcttc gtgatagcca
    99001 atctgaacgc ccacctgacc ggggtattcc gctgccgccg caaggaaacc ttcctgcagc
    99061 accggatcgc ccgcgccgag tagcgccagc tgcccgccct gctccagaag acccggtaag
    99121 gcttccagca ccaggtcgag acctttctgg ctggtcagac ggctcaccac tgcaaaaagc
    99181 ggcactttat cgtcaacctt aagccccatt gcgatttgta actggcgctt attttccgct
    99241 ttatcttcca acgtatcgcg ggtgtaacgc gaggccaaca gtaagtccgt ctctggactc
    99301 cagattttct cgtccacgcc gttcagtacg ccggaaagac gcccttcacg gtgacgctgt
    99361 tgcaacagac cttccatacc gtaggcaaac tgcggttcgg tgatctcgcg agcgtaggtt
    99421 ggactgaccg ccgtaatgtg atcggcatag tacagaccgg ccttcaggaa agagatttgt
    99481 ccgttgaatt ccagcccatg aatattaaag aatgaccatg gcaattggat gtcattcata
    99541 tgatgtgcat aaaacatgcc ttgataggcc aggttgtgca cagtaaacac cgacttcgcc
    99601 ggacgcccgc gcgccgccag atacgcaggc gcaaggcctg catgccagtc gtgcgcatgc
    99661 accacatcag gacgccagaa tgggtcaagc ccgctggcca tttctgcccc aacccacccc
    99721 agcagcgcaa aacgcaatac gttgtcggta taggcaaata agttggtatc gtgatacggg
    99781 cttcccggac gatcatagag atgcggcgcg tcaatcaggt atatgccaac cccgttgtaa
    99841 tgaccgaaca acagcgtgat atgtccggcg aaggtatcac gacgggatac tacctgcgca
    99901 tcggtaacgc cacggcgaat atcgggaaat gcaggcaaca gtacgcgagc gtcaacgccg
    99961 tctgcgattt gtgctgcggg taatgcccca ataacatcag ccagaccgcc ggttttaagc
   100021 agcgggaaca tctctgaaca tacatgtaaa acctgcatta tcgctcctgt ttatgcccta
   100081 acttccgtag catttcgcgc gttaccagca cgatgccttc ttctgaacga tagaaacgac
   100141 gtgcatcttc ctctgcgttt tcaccaatca ccatgccttc cggaataaca caagcacgat
   100201 cgatgacgca gcggcgcaga cggcacgagc gacctaccca tacttccggt aacaatacgg
   100261 cggaatcaat gttgcagaat gaattcacgc gaacgcgcga gaacagaacg gactgcacca
   100321 ccaccgaacc ggagatcaca cagccgccgg ataccagtga gttaagggtc atcccgtggc
   100381 taccggagcg atcctgcacg aatttcgctg gcggtaatga ttcattgtag gtgcgaattg
   100441 gccaattgcg atcgtacata tccagttccg gcaccacaga ggccagatcg aggttcgctt
   100501 tccagtaagc ttccagcgta cccacatcgc gccagtacgg ctcggcatcc gggtcggatt
   100561 gtacgcaaga gagcgggaac gggtgcgcat aggccagacc ggcttcggtg atcttgggaa
   100621 tcaaatcttt gccaaagtcg tggctggagt tctcatcacg atcgtcttct tccagcagtt
   100681 catacagata gtcggcgtca aagacgtaga tacccatact cgccagagat ttgctcggat
   100741 cgttcggcat tgacggtggg ttagcaggtt tttcaacgaa ttcgataatt ttatcgttct
   100801 catcaaccgc cataacgcca aatgcggagg cttcttcaat cggtactggc atacaagcaa
   100861 cggtgcaacg tgcgcctttt tcgacgtgat cgataagcat acgcgagtag tcttgcttgt
   100921 agatatggtc gcccgccagg atcaccacgt attccgcttt ataacggcgg ataatgtcga
   100981 ggttttgggt gaccgcatct gcggtgccgc gataccagtt ttcccctttc attctctgct
   101041 gtgctggcag cagatcgaca aactcgttca tttcttcatt gaagaatgac cagccgcgct
   101101 gaatgtgctg caccagagtg tgggactggt actgggtgat cacgcccata cgacggatcc
   101161 cggagttgat gcagttagac agcgcaaagt cgataatgcg gaacttaccg ccgaagtgta
   101221 cggccggttt tgctcgctta ttggttaaat ccttcaggcg ggtaccacgt cctcccgcca
   101281 gtatcagggc aacagatttc aatggcagct ggcgcgccaa cattaagtga tcgttcttct
   101341 ctaaactaac catgactaac tcctttttta tcatctctgg aacacacaca atccgtgtgc
   101401 aggtccctgc cagacagccg taatcactgg gttatcctct ccagcgaatg ggggaatggc
   101461 gtgccactcc ccagcaggta aaacaatctc tgttacctca agcgtggcgt taattgcgat
   101521 caaaaagcga tccgagagca gaatttgcag ctgtttcggc ccgttttgcc actcatccgt
   101581 gcttaaaggt tgagcatatc gatttagcca acggacattg ccgtcgcctt cttcccacca
   101641 gcgattctcc accaaagcgg gaatgcgctt gcgcagatgg attaacgcgg cggtaaatgc
   101701 ggttaaacca ctgcttgcct gcgaccagtc caaccaggtt aattggttat cctgacagta
   101761 ggcattgtta ttgccatgct ggctgtgacc atgttcgtca ccggccagta acatcggcgt
   101821 accctgggag agcaacaacg ttgttaacag ggcgtgaatg ctgtcgcgcc gccgttcaac
   101881 caggtcaaga gaaccgccta acccttcttt accatgattg ttactgtaat tgttgttggt
   101941 cccgtcgcga ttttcttctc cgtttgcttc attgtgttta tggttgaagc aaacgcagtc
   102001 gcgaagcgta aaaccgtcat gcgcggtgac gagattaatc gcggcactcg gcagacgacc
   102061 attacgttta aaaacatcgc tggaggcagc aaaacgcccg gcaaacgccc ccagaggcaa
   102121 atcataatgt agccagaaac gacgggcagc atcgcggaaa tgatcgttcc actcggcaaa
   102181 cagcggcggg aaatttccca cctgataacc accaggagcg atatcccacg gttcagcaat
   102241 taacttcacc tgcgagagca ccgggcagtt ctggatagcg gtaaacaacg gcgcatcctg
   102301 acggaactct ggcgtacggc ccatgactgc cgccagatca aagcggaaac catcgacgtg
   102361 gcaggtttct acccaataac gcaggcaggc gctggcataa tccaccaccg ccggatgact
   102421 caaattgagc gtgttgccgc aaccggtcca gttgtgataa tcgccgtctt ctcttatcca
   102481 ataatagcta cggttatcga tcccacgcag cgagaataac gggccgtcga ggtccagttc
   102541 cgcactatgg ttgagcacga tatcaagaat gacttcgata cccgctttat gcagtgcttt
   102601 gattgcatcg cgaaactcat ccagcgccgt ttctggcgag caggcatacg ccggatgcag
   102661 cgcaaacatc gccaccgggt tgtaacccca gtagttactt agccccatgc gttgcagacg
   102721 tggttcactg gcaaactgcg ccactggcag cagttccagc gcggtaatgc ccaattgttt
   102781 caaatagttg atcatcaccg gatgcccgag ggctttataa gtgccacgga tctcgaccgg
   102841 gatctccggg tgcaagtacg ttaatccttt gacatgggct tcataaatga tggtgctgcc
   102901 ccacggcgtg cgcggcgggg catcatcttc ccagtcatag tgatcaacca ccactacgca
   102961 tttcggcgca atggcggcgt tgtcgcgata gtcaggttca ttatgaccgg cgtgcagcag
   103021 cgggttatct ttaaactccc cctcaatttg ccgcgcgcaa ggatcaatca acaacttcgc
   103081 cgggttaaag cgatgcccct cggcgggttg ccaggggcca tgaacgcgat aaccataacg
   103141 caaacccggg cgcgcatccg gcagataacc gtgccaaatg tcgccactgt gccctggcaa
   103201 gtcatagcga tgttcctggc cattggcgtc aaagacacac agttctaccc gctcggcatg
   103261 agcggagaaa agtgtgaagt tgacgccctg accgtcgtaa tgcgcgccga tgggagcggg
   103321 tttgccaatg gcgagttgtg tcattctgcc tcccgaacca gccagatagt ggccagcggt
   103381 ggtagcgtca ggcttagtga atgctgacga ccgtggctgg caatctcatc gctgtgtacc
   103441 gtgccgccat tgcctgcatt actgccgtga tagtgcatgg aatcggtatt gaggatttca
   103501 cgccatttgc ccggctggtt tatgccgaag cgataatcat gacgcggcac cggcgtaaag
   103561 ttactggcaa cgatgatttc gttaccctct ttatcgcgac gcacaaagat cagcaccgag
   103621 cgttctttgt catccaccac cagccattca aagccgtacg ggtcaaaatc cagttcatgc
   103681 attgctttat ggtggcggta ggtgaggttc agatcgcgca ccagacgctg gacaccgtgg
   103741 tgccagttat cgccgccttc caacagatgc cagtcgaggc tggcgtcatg gttccactcg
   103801 cggccctggg caaattcgtt acccatgaac agtagtttct tgcccgggaa tgcccacatc
   103861 cagccatagt aggcgcgcag gttcgcgaat ttctgccatg cgtcgcccgg catgcggtcg
   103921 agaatcgatt ttttaccgtg gaccacttca tcatgcgaca acggcaggac gaagttttca
   103981 gtgtagttgt agagaatccc gaaggtcagt ttatcgtgat gatactgacg ataaaccggg
   104041 tcgagcttca tgtagtccag ggtgtcatgc atccagccga ggttccactt gtaccagaag
   104101 cccagaccgc ccatatcctg cggacgagaa acgccaggga aatcggtaga ctcctcagcc
   104161 attgtcaccg caccggaaac ctgctcacca agaatacggt tggtattacg caagaattca
   104221 atcgcttcaa gattctcgcg cccgccaaat tcgttcggga tccactcccc ctctttacgg
   104281 ctgtagtcgc gataaatcat tgacgccacc gcatcgacgc gcagcgcatc aataccaaaa
   104341 cgttcaatcc agtaaagcgc gttaccgacg aggaagttac tgacttcacg gcgaccatag
   104401 ttgtagatca gcgtgttcca gtcctgatga tagccttcac gcggatcgct gtgttcatac
   104461 aagttcgtgc catcaaattc ggcaagcgca aagtcatcag tcgggaagtg gcctggcacc
   104521 cagtcgagaa tcacgttcag accagctgcg tgtgcggcat caatgaaata acggaagtcg
   104581 tcgcgagtac caaaacggcg ggttggtgca tacaggccgg ttggctgata accccaactg
   104641 ccatcgaagg gatgctcgtt aatgggcagt agttcgaggt gggtaaagcc catccattta
   104701 gcataaggca ccagttgatc ggccagctcg cggtagctca accagaaatt gttgtcggtg
   104761 tggcgacgcc aggaacccag gtgaacttca taaatagaga ttggcgcatc aaactgattc
   104821 gcttttttgc gctcttcagt ctgtacaacc ttttccggca gcccgcaaat aagagacgcg
   104881 gtttccgggc gcatttgcgc ttcgaaggca taagggtcgg acttcagacg caagttgcca
   104941 ttggcatcaa tcatctcgta tttatagagc tgaccgttat gcgccccagg gataaacagt
   105001 tcccagatgc cgctctcttt acgcaggcgc atcgggtgac ggcgaccgtc ccagtagttg
   105061 aattgcccaa ccaccgagac ccgacgggcg tttggagccc agacagagaa acgcgtacct
   105121 gtgacgccat ccatagtatc tgcatgcgcg cctaaggttt catacgggcg caggtgagta
   105181 ccttcagata atagccaggc atccatttcc tggattagcg gaccaaaacg gtaaggatca
   105241 tcaatcaggt tttgctgacc atgccagaca acagccaact gatagcggaa aaaattctta
   105301 cgtcgcggaa tgacgccgct aaagaatccc cgtgagtcga gacactccag ttttgcgagt
   105361 ttgcgcccgg ttttcggttc aatcacccac acatcggtag cgtcgggtaa aagggcacgg
   105421 acttccagtc ccgcggtggt tttatgcatt cccagtacgg aaaaaggatc cgcaaaatgg
   105481 cctgcaatta gcgcgttaat cacgtctcta tcgatacgat cggacatgct tgtcttcctg
   105541 ttttattgtg tcaccccatc caattggatt tttgacttct ggttgtgaca tttttttgac
   105601 ctgaacggcg cagcactctg tgcatcctct ctgcgtcgtc ctcacttcag gtaaggctgt
   105661 gaatactcat gtattcagcc acccttaaag aatagccaat gctctattta actcccggta
   105721 aatcatgaaa catctgcgct tactcctgta ttacgcacta acaggggcgg catcgcgccc
   105781 cagatttaat gaataaagat tacgccagtt gacgaagcat ccgacgcagc ggctccgcgg
   105841 ccccccacag cagctggtcg cccacggtaa aggctgacag gaactctggt cccatattca
   105901 gcttacgcag gcggcctacc ggcgtggtca gcgtgccggt aacggcagct ggggttagct
   105961 cacgcatagt gatttcccga tcgttcggaa cgactttcgc ccacggattg tgcgcagcca
   106021 gcagttcttc cacggtcgga atagacacat cttttttcaa tttaatagtg aatgcctggc
   106081 tgtggcagcg caatgccccg acacgcacac ataaaccatc taccggaatt acggaagatg
   106141 tgttgaggat cttgttggtt tccgcctgcc ctttccactc ttcgcggctc tgaccgttat
   106201 cgagctgttt gtcgatccac ggaatcaggc tacccgccag cggcacgcca aagttatcca
   106261 ccggcagctc accgctacgg gttaaggttg tgactttgcg ttcgatatcg agaatagcag
   106321 aggacggggt cgcgagttca tctgccacat ggccatacag atggcccatc tgggttaata
   106381 actcacgcat atgtcgcgca ccaccgccgg aagcggcttg gtaggttgca acggacaccc
   106441 aatcaacaag atcattggcg aataaaccac ccaacgacat caacatcagg cttacggtac
   106501 agttaccgcc aacaaaagtc ctgatgccat tatttaatcc gtcggtaatg acgtcctgat
   106561 tgacggggtc aagaatgatg atggcgtcat ctttcatgcg cagagacgat gctgcgtcaa
   106621 tccagtaacc ttgccatccg ctttcacgaa gctttggata gatttcgttg gtataatcgc
   106681 cgccctgaca ggtcacaatg atatcgaggg cctttagcgc ctccagatca aaggcatcct
   106741 gaagtgtgcc agtggttccg ccaaaagacg gcgcagcctg gccaagctga gaagtagaaa
   106801 agaagacagg gcgaatggcg tcgaagtcgc gctcttcaac catgcgttgc atgagaacgg
   106861 agccgaccat accgcgccag ccgataaaac caacattttt cataagcgtt tttttcctgc
   106921 aaagatgtgt gctgtataaa tgtgccggtc tcctcttggc acatctttca ccatacaaaa
   106981 agcagccaaa gtcgcaagtg aaattaatca atgatagcga agccatcagt aatgcgactt
   107041 atcctgcttt gttagcacgc agaaagtccg cggcaattat cagggaattt gagttatgaa
   107101 tgaaatcatt tctgcagcag ttttattgat cctgattatg gatccgctcg gaaacctacc
   107161 tattttcatg tccgtactga aacatactga accgaaaaga cggcgggcaa tcatggtgcg
   107221 agagttgctt attgctctcc tggtgatgct ggtgttcctg tttgcgggtg agaaaattct
   107281 ggcatttctt agcctacgag cagaaaccgt ctccatttct ggcggcatca ttctgtttct
   107341 gatcgccatt aaaatgattt tccccagcgc ttcaggaaat agcagcgggc ttccggcagg
   107401 tgaagagcca tttatcgtgc cgttggcaat tccgttagtc gccgggccga ctattctcgc
   107461 cacgctgatg ttgttgtctc atcagtaccc gaatcagatg gggcatctgg tgattgctct
   107521 gctgctggcc tggggcggca cctttgtcat cctgctacag tcttcgctat ttttacgtct
   107581 gctgggcgag aaaggggtga acgcacttga acgcctgatg ggattgattc tggtgatgat
   107641 ggcaacccag atgttcctcg acggcattag aatgtggatg aaggggtaac gaggagagtt
   107701 tttcgaaaag aacccggtag cgtcacaact accgggcgaa caaacttaac tcaacagctg
   107761 gaacgcaatc atcccaacga tggcaccgac agtgccgagg atggtttcca tcatggtcca
   107821 ggttttcagc gtttcggctt cggtcgcgcc ggtaaattta ccgaacaacc agaaaccggc
   107881 gtcgttaacg tggctgacaa caatcgaacc accagcgata caaatcgaca gcgccgccat
   107941 ttgcgcaccg gagtagttca gttgttcaat aaccggcatc accagtccta ccgccgttaa
   108001 acaggctacg gtggcagaac cctgaatgat gcgcactgca gctgccagca cgaagcaggt
   108061 gatggctatc ggcaggccca tgccggttaa cgcttcgccc agtgccggac ctacgccaga
   108121 gtcaaccagc acctgtttga acacgccgcc tgcaccaatc accagcagaa tgatccccgc
   108181 cggttgcagc gcgtgaccgc aaatctccat cactttgtct tttggcatgc cctgacgcat
   108241 tgccaggcca taaatcgcta ccagacaagc aaccagaatc gcggtaaacg gatgaccaat
   108301 aaactcgaac cattcgtaag cggtagagcc ttctggcaca aaacgcgcgg caatggtttt
   108361 cagccccacc agcaccagcg gcaacaggat cagcgacagg ctgaatccga aagatggcat
   108421 tttgccttcg ccgagatgcg gttcgctgat gtcgtcagga atatgcaact ccacgtagcg
   108481 gctgatgaaa ttaccccaca gcggcccggc aataatcatt cccggaattg ccgcacacag
   108541 gccaatcagg atcatccagc caaagtcggc attcatctgc gatgccagca gcatcggcgc
   108601 tggtccaggc accagaaatg ccgccgctgc cgccacgcct gcaaataatg ggattaccag
   108661 cttcaccagg ttcgtaccgg tgtggcgcgc cattgagaaa gcaacgctaa tcagcagaac
   108721 tatcgccact tcaaagaaca gcggtagcgc acagaccagc cccgcaaggc cgatggcata
   108781 atgcgcgcgg ctgtgaccga aggatttgag cattttgacg gcaatctgat cgactgcgcc
   108841 ggtttcatgt aagatcttgc caaacatagc tcccagggcg acaaccaccg ccaggaagcc
   108901 gagggtgcct cccatccctt tttccatcgt cgctgcgatt ttatcgagcg gcataccgga
   108961 aaaaaggcca gcccccatgg acaccaccat caaagccagg aaagcgtgca tacgcgcctt
   109021 catgactaaa aacagcagca gtaaaacaga ccctactgct gttaaaacaa gcgttaatgt
   109081 agtcactact tatttgcctt ttttaataac ctcaatggtg cttgccacaa caccttccag
   109141 cggttgatcg atatccacca tcagtacatc ggtttcgtcc gcacccggct cctgcagcgt
   109201 ttcaaactgc gtcaccaaca tttgggtttt aaagaaatgg cctttgcgcg ctttcaggcg
   109261 gctttcaatc acatcaaaat cgcctttcag gtagatgaaa gagagattcg gattaccttc
   109321 acgcagcaag tcgcgatagt gttttttcaa tgcagaacag acgatcagtg acactttatt
   109381 agtgcgctgc atagcaaacg cggcgtcgtt cagcgcctgc aaccacggtt tgcgatcgtc
   109441 gtcattcagc ggttcgccag acgccatttt ttcgatattg cgccgtggat ggaggaaatc
   109501 gccatcaaga aacgcggcat gaagttgatg cgccacttca ctggcgaccg cagatttgcc
   109561 gctgcctgag acgcccatca agacgtaaat gtggtgatca tggttagtcg tgctcaaagt
   109621 ggtgccccca caatacaaga attaaaaatg ttacgggtaa ctgttatcgg taacattgtc
   109681 cggccggaca atatcagaag caatatccat ccgtgcctta agtgtataag tgtgagctac
   109741 ttcaaatttg tgggcttaaa tagatccgcc cggtgacaag gtgaaaccta aatctaacat
   109801 tttcggtgtc acagattcgc cacgaatacg cgccagcagg cgttcagcgc caatactgcc
   109861 catccgctca cgcggcgtca gcacgctcgc cagtcgtggc tccatcacct gaccaatgtc
   109921 atgaccgtgg aaaccggcaa tcgccatatc gtcaggaact tttaacccca gacgctgaca
   109981 ttcaaacgcc gcgccgaccg ccagatcatc attagtacag aacacgccat ccagctgcgg
   110041 atattcccgc ctcgcctggc gaatcagttc aataccggaa gagtaagaag aagattgctc
   110101 aaccatcacg ctatatggca ccaggtctgc atccagcatc gcctgttcgt atcccttctg
   110161 tttgatgata gtacgttcgt cgagacgtgc gccgagataa gcaatgtggc gatgcccgcg
   110221 agcaataatg gcggtggtca tctggcgtgc cgcttcaaag ttatcaaaac cgacggcaat
   110281 atcgaggcac ggcgacttgc tgtccatcaa ctccaccacc gggatccccg ccacttcaat
   110341 catctttaag gtgcgtggcg tgtgagtacg ttcggtgagg atcagtccat cgatattcca
   110401 tgagagcatg gattcgaggc gttcttgctc catttccggt ttataaccgt agtgcgccag
   110461 catggtttga taaccgtgcg cgtcggtgac gctttcgatt ccgcgtaata cttccgcgaa
   110521 aacctggttg gtgagagaag gtaacaggac gccaatcgcc cggctggtgg cgttagagag
   110581 gatatcgggc gcacgattgg gaatataacc cagttcatca agagcggcgg caatcttgcc
   110641 gcgtagagcg acggaaacct gctccgggtt acgcaaaaag cggctgaccg tcattttggt
   110701 cacgcctaca cggtcagcca catcctgaag tacgggtctt ttctttttca tcgtcctgaa
   110761 ggtacaaaag agatagattc tcttagttta acacggacgc atcagaacct tcccggggaa
   110821 aacagtgctt gtttacaatt attagatata cactaaatca attgcatcgc attgtgctaa
   110881 cgacgcggca aaaccccgga agcgtgcatc agcacgcgac cggagcgagc gaggaagcct
   110941 gcaaaaaggc aggcttcaaa aatgccgttg agttttaaac cggcggcaga tcgaacagta
   111001 acacttcgct gtcgctatcc gcatggatgg agattgcctg ctcatcccag attgccagac
   111061 catcgctggt cgaggctttc acgccgttaa tggtgacatt gcctttcacc acctggatcc
   111121 agacgcggcg ttcagcggca atctgatgca ccgactgctc atctttcagc aacgcccaac
   111181 gatacagttc catatcctga tgcactttca gcgaaccatc tcgcgcatcc ggcgagagca
   111241 ccagctgttt gccctgcacg gcatcgaagc gacgctgttc ataacgcggc gtaatgccat
   111301 tttcttcagg catgatccag atctgataca gatgcagacg ctcggtgctg cttgggttgt
   111361 actctgagtg acgaataccc gtacccgcac tcataatctg gaactcaccc gccggaacct
   111421 gctctttatt gcccatgctg tcctgatgct caacagtacc ttccagcacg taggtcaaaa
   111481 tttccatatc tttatgcggg tgagtgccga agccctgccc tgcttcaatc acgtcgtcgt
   111541 taatcacgcg cagcgcggag aagcccataa agttcggatc gtaatagttg gcaaaagaga
   111601 aagtatgcca ggagtccagc cagccatgat ttgcatgacc acgttcattt gctttgcgta
   111661 agtagatcat ttgtattcgc cccctgaatg atttcgatgg gcttagtgtg gacccgatct
   111721 gcctgggatg atagagggtg aaaactgacc cctctgttca aaaaaattga actattcgag
   111781 gggccatcaa acttacttag cgagagttac tgtggaggga gaggcttgct caaatccgcg
   111841 ttcaaggatt tccagattgg taagaacttc agattccttg acgtaatttg gcgcaccgtg
   111901 ggtgatggtt tgatacaacg catcataaac gcgcccgtaa tcgcccatct ccggcttcat
   111961 ctcttctctg accgtcacgc cctcgtcatt gacatactcc agcacaccga ccgaattatc
   112021 cgctgcgaat cccggttcgc ccggcataat attagccttc aggctggttt cctgctggtc
   112081 gataccgtat ttaataaacg aacctttctt accgtgaacg ataaatttcg gataatcgat
   112141 tttcaccaga tggctggttt tgacgatggc tttcaggtcg ccataaaaca gttgcgcttc
   112201 aaaggtgtcg tcaggattgg ctttattacg caggctgcgg atgtcataag cgacgtgatc
   112261 cgggcgaccg aacagagaaa taatctggtc catcgtatgc acaccaaggc cataaaacgc
   112321 gccatcctgc ggcagcccag gtttggtttc tgccaccggg cggtaatagt caaaatggct
   112381 ttccacttca acaatctctc ccaacttgcc actttcaatc gcttttttcg ctgtcaggaa
   112441 gcaggagtca aagcgacgat tctgatacgg cgtgacggtc agccctttgc ttttcgccaa
   112501 cgcaaacagc tctttcgcct gcgcaagtgt cggagtgaac ggtttttcga ccagcacatt
   112561 tttcccggct tccagcgcgc gcttcgcgta ctcgaagtgg ctgtccgcgt gggtgcagac
   112621 aacaaccagc ttaacatcgg gatcgtttag tacttcgtcg agatcgctgg tgaaatggat
   112681 atgggaataa atgggagcct gttcttccgg cttcgcatgg cgacgaaaaa tatgcgcgac
   112741 atgccaacta tccttgcggt taagtacata cggcagatgg taacgggtgg tgcttttgcc
   112801 gaagccaata aaggcgcagt tgatgaccat gatttcgtcc tttttaaggt ggttatttac
   112861 accttagcgc aaagcggacg tggttcctac tggagtgcgc ataacgaaca caagcactcc
   112921 cgtggataaa ttgagaacga aagatcaaaa aaaaagccag cacccggctg gctaagtaat
   112981 actggaagca atgtgagcaa tgtcgtgctt tcaggttctc cgcgagggtc ttcctgatcg
   113041 cgagacaata ataatcattc tcattcgcac ttgtccaaca ctttttgcaa aaaaatgcat
   113101 ttgactcgca tttgaaagtc aatgatgttg aaagggacat ttaccccaaa gaggacaaag
   113161 gaatgagtga gatagtaata cgccacgcag aaacacggga ttacgaggcc atcaggcaga
   113221 ttcacgccca gccggaggtg tattgcaaca cactacaggt gcctcatcct tccgatcata
   113281 tgtggcagga gcgactcgcc gatcgtcccg gcatcaagca actcgtcgcc tgtattgatg
   113341 gagacgtcgt gggccatctc accattgacg tgcaacagcg cccacgccgc agtcatgttg
   113401 ccgattttgg tatctgtgtc gactctcgtt ggaagaaccg cggcgtcgcc agcgccctga
   113461 tgcgagagat gattgaaatg tgcgacaact ggttgcgggt agatcgcatt gaactaaccg
   113521 tgtttgtcga taacgccccg gcaattaagg tctataaaaa atacggcttt gaaattgaag
   113581 ggactggtaa gaagtacgca ttgcgtaatg gtgaatatgt cgatgcatat tatatggcgc
   113641 gggtgaagta agatagtgcc ctttttctga gatggaaaaa gggtgtcatt caaaatcgac
   113701 ataccttcct ttaaggtatt tatttgccca atacatatat tgagatttaa ctcataatta
   113761 cttcctaaag tgtaatattt tattttttaa tatatacgcc tacaatttcc tggagtaaat
   113821 aaataacaat taacaagcat aatattgcca ttgataaaat agcatgccat aaaaggactt
   113881 ttcagggatg agtaatattg tttacctgac agtaacggga gaacaacaag gaagcatctc
   113941 cgcaggttgt gggacttctg agtctacagg taatcgttgg cagagcgggc atgaggatga
   114001 aatatttaca ttctcactct taaataatat taataatacg gggcttggtt cacagttcca
   114061 tggtataaca ttttgtaaat taattgataa aagcactcca ttatttatta attccattaa
   114121 caataatgaa caattattta tgggatttga cttctatcga ataaatagat ttggtagatt
   114181 ggaaaagtat tattatatac aactaagagg cgctttttta tcggctattc atcaccagat
   114241 cattgaaaac caactggata cagaaacaat aactattagt tatgaattta tcctctgtca
   114301 acatcttatc gcaaataccg agttcagcta tttggcactc cctgaaaatt ataaccgttt
   114361 gtttttacca aattcaaaaa accaaacaaa taatcgtttc aaaacgttaa acagcaaagc
   114421 tattggcagg ctacttgctg ctggtggcgt atacaatggg aacattgaag gattcagaga
   114481 tactgcggaa aaactgggtg gagatgcaat aaaaggctat gatcaaatac taaatgaaaa
   114541 aacagcgggc atagcgatag caacagcatc tattctttta acaaagcgtt ctaatgttga
   114601 tacatataca gaaataaata gttacttagg caaacttaga ggtcaacaaa
//
LOCUS       DAZFQD010000002        95615 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.002, whole genome shotgun sequence.
ACCESSION   DAZFQD010000002 DAZFQD010000000
VERSION     DAZFQD010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 95615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 95615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..95615
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.002"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            13..531
                     /gene="yfgH"
                     /locus_tag="ACHKTW_000106"
     CDS             13..531
                     /gene="yfgH"
                     /locus_tag="ACHKTW_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HFO6461202.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAVGSMVNDKTLVEGVSLTYKEGTKVYTSTQVGKECQFTTGLAVVITTTYNET
                     RIQPNTKCPEKS"
     gene            547..1086
                     /gene="yfgI"
                     /locus_tag="ACHKTW_000107"
     CDS             547..1086
                     /gene="yfgI"
                     /locus_tag="ACHKTW_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HFO6461203.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEASADKKRDQSYE
                     DELRSLEIQKQKLALAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(1181..2758)
                     /gene="guaA"
                     /locus_tag="ACHKTW_000108"
     CDS             complement(1181..2758)
                     /gene="guaA"
                     /locus_tag="ACHKTW_000108"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFO6461204.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPVFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(2827..4293)
                     /gene="guaB"
                     /locus_tag="ACHKTW_000109"
     CDS             complement(2827..4293)
                     /gene="guaB"
                     /locus_tag="ACHKTW_000109"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFO6461205.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            4455..5825
                     /gene="xseA"
                     /locus_tag="ACHKTW_000110"
     CDS             4455..5825
                     /gene="xseA"
                     /locus_tag="ACHKTW_000110"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFO6461206.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDQQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTAVQGDDAPGQIVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     TRQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRTGQQQQRLTQRLNQQNPQPKIHRAQTRIQQLEYRLAETLRVQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKAGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            complement(5822..6037)
                     /gene="yfgJ"
                     /locus_tag="ACHKTW_000111"
     CDS             complement(5822..6037)
                     /gene="yfgJ"
                     /locus_tag="ACHKTW_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HFO6461207.1"
                     /translation="MELHCPQCQHVLDQDNDHARCPSCGEIIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(6106..7578)
                     /gene="der"
                     /locus_tag="ACHKTW_000112"
     CDS             complement(6106..7578)
                     /gene="der"
                     /locus_tag="ACHKTW_000112"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFO6461208.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVANKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            complement(7696..8874)
                     /gene="bamB"
                     /locus_tag="ACHKTW_000113"
     CDS             complement(7696..8874)
                     /gene="bamB"
                     /locus_tag="ACHKTW_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HFO6461209.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWAQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            complement(8885..9505)
                     /gene="yfgM"
                     /locus_tag="ACHKTW_000114"
     CDS             complement(8885..9505)
                     /gene="yfgM"
                     /locus_tag="ACHKTW_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HFO6461210.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGVILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            complement(9523..10797)
                     /gene="hisS"
                     /locus_tag="ACHKTW_000115"
     CDS             complement(9523..10797)
                     /gene="hisS"
                     /locus_tag="ACHKTW_000115"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFO6461211.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGISEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELPGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            complement(10908..12026)
                     /gene="ispG"
                     /locus_tag="ACHKTW_000116"
                     /gene_synonym="gcpE"
     CDS             complement(10908..12026)
                     /gene="ispG"
                     /locus_tag="ACHKTW_000116"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity (ferredoxin) [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HFO6461212.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQQVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVEK"
     gene            complement(12053..13066)
                     /gene="rodZ"
                     /locus_tag="ACHKTW_000117"
     CDS             complement(12053..13066)
                     /gene="rodZ"
                     /locus_tag="ACHKTW_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HFO6461213.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPAPTATTTPDGAAPLPTDQAGVTTPAADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            complement(13351..14505)
                     /gene="rlmN"
                     /locus_tag="ACHKTW_000118"
     CDS             complement(13351..14505)
                     /gene="rlmN"
                     /locus_tag="ACHKTW_000118"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HFO6461214.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKPFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPGAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            complement(14655..15086)
                     /gene="ndk"
                     /locus_tag="ACHKTW_000119"
     CDS             complement(14655..15086)
                     /gene="ndk"
                     /locus_tag="ACHKTW_000119"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFO6461215.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            complement(15235..17547)
                     /gene="pbpC"
                     /locus_tag="ACHKTW_000120"
     CDS             complement(15235..17547)
                     /gene="pbpC"
                     /locus_tag="ACHKTW_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="penicillin-binding protein 1C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
                     /protein_id="HFO6461216.1"
                     /translation="MPRLLTKRGYWITLAAAPFLLFLAAWGADKLWPLPLHEVNPARV
                     VVAQDGTPLWRFADADGIWRYPVTIEDVSPRYLEALINYEDRWFWKHPGVNPFSVARA
                     AWQDLTSGRVISGGSTLTMQVARLLDPHPKTFGGKIRQLWRALQLEWHLSKREILTLY
                     LNRAPFGGTLQGIGAASWAYLGKSPANLSYSEAAMLAVLPQAPSRLRPDRWPERAEAA
                     RNKVLERMAVQGVWSREQVKESREEPIWLAPRQMPQLAPLFSRMMLGKSKSDKIVTTL
                     DAGLQRRLEELAQNWKGRLPPRSSLAMIVVDHTDMRVRGWVGSVDLNDDSRFGHVDMV
                     NAIRSPGSVLKPFVYGLALDEGLIHPASLLQDVPRRTGDYRPGNFDSGFHGPISMSEA
                     LVRSLNLPAVQVLEAYGPKRFAAKLRNVGLPLYLPNGAAPNLSLILGGAGAKLEDMAA
                     AYTAFARHGKAGKLRLQPDDPLLERPLMSSGAAWIIRRIMADEAQPLPDSALPRVAPL
                     AWKTGTSYGYRDAWAIGVNARYVIGIWTGRPDGTPVVGQFGFASAVPLLNQVNNILLS
                     RSANLPEDPRPNSVTRGVICWPGGQSLPEGDGNCRRRLATWLLDGSQPPTLLLPEQEG
                     INGIRFPIWLDENGKRVAADCPQARQEMINVWPLPLEPWLPASERRAVRLPPASTICP
                     PYGHDAKLPLQLTGVRDGAIIKRLPGAAEATLPLQSSGGAGERWWFLNGEPLTERGRN
                     VTLHLTDKGDYQLLVMDDVGQIATVKFVMQ"
     gene            complement(17548..22509)
                     /gene="yfhM"
                     /locus_tag="ACHKTW_000121"
     CDS             complement(17548..22509)
                     /gene="yfhM"
                     /locus_tag="ACHKTW_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha2-macroglobulin"
                     /protein_id="HFO6461217.1"
                     /translation="MKKLRVAACMLMLALAGCDNNDNAPTAVKKDAPSEVTKAASSEN
                     ASSAKLSVPERQKLAQQSAGKVLTLLDLSEVQLDGAATLVLTFSIPLDPDQDFSRVIH
                     VVDKKSGKVDGAWELSDNLKELRLRHLEPKRDLIVTIGKEVKALNNATFSKDYEKTIT
                     TRDIQPSVGFASRGSLLPGKVVEGLPVMALNVNNVDVNFFRVKPESLPAFISQWEYRN
                     SLANWQSDKLLQMADLVYTGRFDLNPARNTREKLLLPLGDIKPLQQAGVYLAVMNQAG
                     RYDYSNPATLFTLSDIGVSAHRYHNRLDIFTQSLENGAAQQGIEVSLLNEKGQTLTQA
                     TSDAQGHVQLENDKNAALLLARKDGQTTLLDLKLPALDLAEFNIAGAPGYSKQFFMFG
                     PRDLYRPGETVILNGLLRDADGKALPNQPIKLDVIKPDGQVLRSVVSQPENGLYHFTW
                     PLDSNAATGMWHIRANTGDNQYRMWDFHVEDFMPERMALNLTGEKTPLTPKDEVKFSV
                     VGYYLYGAPANGNTLQGQLFLRPLREAVSALPGFEFGDIAAENLSRTLDEVQLTLDDK
                     GRGEVSTESQWKETHSPLQVIFQGSLLESGGRPVTRRAEQAIWPADALPGIRPQFASK
                     SVYDYRTDSTVKQPIVDEGSNAAFDIVYSDAQGVKKAVSGLQVRLIRERRDYYWNWSE
                     DEGWQSQFDQKDLIENEQTLDLKADETGKVSFPVEWGAYRLEVKAPNEAVSSVRFWAG
                     YSWQDNSDGSGAVRPDRVTLKLDKASYRPGDTIKLHIAAPTAGKGYAMVESSEGPLWW
                     QEIDVRAQGLDLTIPVDKTWNRHDLYLSTLVVRPGDKSRSATPKRAVGVLHLPLGDEN
                     RRLDLALETPAKMRPNQPLTVKIKASTKNGEKPKQVNVLVSAVDSGVLNITDYVTPDP
                     WQAFFGQKRYGADIYDIYGQVIEGQGRLAALRFGGDGDELKRGGKPPVNHVNIVAQQA
                     LPVTLNEQGEGSVTLPIGDFNGELRVMAQAWTADDFGSNESKVIVAAPVIAELNMPRF
                     MASGDTSRLTLDITNLTDKPQKLNVALTASGLLELVSDSPAAVELAPGVRTTLFIPVR
                     ALPGYGDGEIQATISGLALPGETVADQHKQWKIGVRPAFPAQTVNYGTALQPGETWAI
                     PADGLQNFSPVTLEGQLLLSGKPPLNIARYIKELKAYPYGCLEQTASGLFPSLYTNAA
                     QLQALGIKGDSDEKRRASVDIGISRLLQMQRDNGGFALWDKNGDEEYWLTAYVMDFLV
                     RAGEQGYSVPTDAINRGNERLLRYLQDPGMMSIPYADNLKASKFAVQSYAALVLARQQ
                     KAPLGALREIWEHRADAASGLPLLQLGVALKTMGDATRGEEAIVLALKTPRNSDERIW
                     LGDYGSPLRDNALMLSLLEENKLLPDEQYTLLNTLSQQAFGERWLSTQESNALFLAAR
                     TIQDLPGKWQAQTSFSAEPLTGEKTLNSNLNSDQLATLQVRNSGDQPLWLRMDASGYP
                     QSAPLPANNVLQIERHILGTDGKSKSLDSLRSGDLVLVWLQVKASNSVPDALVVDLLP
                     AGLELENQNLANGSASLEQSGGEVQNLLNQMQQASIKHIEFRDDRFVAAVAVDEYQPV
                     TLVYLARAVTPGTYQVPQPMVESMYVPQWRATGTAEDLLIVRP"
     gene            22716..23561
                     /gene="sseA"
                     /locus_tag="ACHKTW_000122"
     CDS             22716..23561
                     /gene="sseA"
                     /locus_tag="ACHKTW_000122"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HFO6461218.1"
                     /translation="MSTTWFVGADWLAEHIDDPEIQIIDARMASPGQEDRNVAQEYLN
                     GHIPGAVFFDIEALSDHTSPLPHMLPRPETFAVAMRELGVNQDKHLIVYDEGNLFSAP
                     RAWWMLRTFGVEKVSILGGGLAGWQRDDLLLEEGAVELPEGEFNAAFNPEAVVKVTDV
                     LLASHENTAQIIDARPAARFNAEVDEPRPGLRRGHIPGALNVPWTELVREGELKTTDE
                     LDAIFFGRGVSYDKPIIVSCGSGVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLP
                     VEPVK"
     gene            23897..24022
                     /gene="timP"
                     /locus_tag="ACHKTW_000123"
     CDS             23897..24022
                     /gene="timP"
                     /locus_tag="ACHKTW_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small toxic inner membrane protein TimP"
                     /protein_id="HFO6461219.1"
                     /translation="MKIRCFCIVLIVSGALFSQVNNNRSLSGDNLLVVNNLQSSK"
     gene            complement(24054..24830)
                     /gene="sseB"
                     /locus_tag="ACHKTW_000124"
     CDS             complement(24054..24830)
                     /gene="sseB"
                     /locus_tag="ACHKTW_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HFO6461220.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKSVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQATGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIVPFYERRWGGFLRDFKQNRII"
     gene            complement(24972..26255)
                     /gene="pepB"
                     /locus_tag="ACHKTW_000125"
     CDS             complement(24972..26255)
                     /gene="pepB"
                     /locus_tag="ACHKTW_000125"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HFO6461221.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKAPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAITRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPELIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            complement(26433..26633)
                     /gene="iscX"
                     /locus_tag="ACHKTW_000126"
     CDS             complement(26433..26633)
                     /gene="iscX"
                     /locus_tag="ACHKTW_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HFO6461222.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            complement(26645..26980)
                     /gene="fdx"
                     /locus_tag="ACHKTW_000127"
     CDS             complement(26645..26980)
                     /gene="fdx"
                     /locus_tag="ACHKTW_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HFO6461223.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            complement(26982..28832)
                     /gene="hscA"
                     /locus_tag="ACHKTW_000128"
     CDS             complement(26982..28832)
                     /gene="hscA"
                     /locus_tag="ACHKTW_000128"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HFO6461224.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQAGIPDRSDNRVQRELLDAAIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNQLIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDDAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            complement(28849..29364)
                     /gene="hscB"
                     /locus_tag="ACHKTW_000129"
     CDS             complement(28849..29364)
                     /gene="hscB"
                     /locus_tag="ACHKTW_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0097428 - protein maturation by iron-sulfur
                     cluster transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HFO6461225.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSQAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNEAWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            complement(29460..29783)
                     /gene="iscA"
                     /locus_tag="ACHKTW_000130"
     CDS             complement(29460..29783)
                     /gene="iscA"
                     /locus_tag="ACHKTW_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HFO6461226.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            complement(29800..30186)
                     /gene="iscU"
                     /locus_tag="ACHKTW_000131"
     CDS             complement(29800..30186)
                     /gene="iscU"
                     /locus_tag="ACHKTW_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HFO6461227.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            complement(30214..31428)
                     /gene="iscS"
                     /locus_tag="ACHKTW_000132"
     CDS             complement(30214..31428)
                     /gene="iscS"
                     /locus_tag="ACHKTW_000132"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HFO6461228.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            complement(31540..32028)
                     /gene="iscR"
                     /locus_tag="ACHKTW_000133"
     CDS             complement(31540..32028)
                     /gene="iscR"
                     /locus_tag="ACHKTW_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HFO6461229.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRIQDAIDVKL
                     RA"
     gene            complement(32329..33066)
                     /gene="trmJ"
                     /locus_tag="ACHKTW_000134"
     CDS             complement(32329..33066)
                     /gene="trmJ"
                     /locus_tag="ACHKTW_000134"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HFO6461230.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKA"
     gene            33185..33988
                     /gene="suhB"
                     /locus_tag="ACHKTW_000135"
     CDS             33185..33988
                     /gene="suhB"
                     /locus_tag="ACHKTW_000135"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0046854 - phosphatidylinositol phosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HFO6461231.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAMLANMRDELSDALKR"
     gene            34133..34987
                     /gene="yfhR"
                     /locus_tag="ACHKTW_000136"
     CDS             34133..34987
                     /gene="yfhR"
                     /locus_tag="ACHKTW_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417029.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO6461232.1"
                     /translation="MALPVNKRVPKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQRLVLFGQSIGGA
                     NILDVIGRGDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHGDVYREQMVDFI
                     LSALNPQN"
     gene            35178..36458
                     /gene="csiE"
                     /locus_tag="ACHKTW_000137"
     CDS             35178..36458
                     /gene="csiE"
                     /locus_tag="ACHKTW_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HFO6461233.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATTATFSELNGVDD
                     DIASLDISETGQEILRYHQLTLTAGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSHFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFETRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQPVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYTHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(36450..37589)
                     /gene="hcaT"
                     /locus_tag="ACHKTW_000138"
     CDS             complement(36450..37589)
                     /gene="hcaT"
                     /locus_tag="ACHKTW_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HFO6461234.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLTLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     ALLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQGSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(37749..38639)
                     /gene="hcaR"
                     /locus_tag="ACHKTW_000139"
     CDS             complement(37749..38639)
                     /gene="hcaR"
                     /locus_tag="ACHKTW_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HFO6461235.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KPALRDFIAIVQERLASVTA"
     gene            38775..40136
                     /gene="hcaE"
                     /locus_tag="ACHKTW_000140"
     CDS             38775..40136
                     /gene="hcaE"
                     /locus_tag="ACHKTW_000140"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit alpha"
                     /protein_id="HFO6461236.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNTRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLRDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            40133..40651
                     /gene="hcaF"
                     /locus_tag="ACHKTW_000141"
     CDS             40133..40651
                     /gene="hcaF"
                     /locus_tag="ACHKTW_000141"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HFO6461237.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLAQLDEEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QISETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            40651..40971
                     /gene="hcaC"
                     /locus_tag="ACHKTW_000142"
     CDS             40651..40971
                     /gene="hcaC"
                     /locus_tag="ACHKTW_000142"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HFO6461238.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            40968..41780
                     /gene="hcaB"
                     /locus_tag="ACHKTW_000143"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             40968..41780
                     /gene="hcaB"
                     /locus_tag="ACHKTW_000143"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HFO6461239.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAAWYPGGGGPLYTASK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            41790..42992
                     /gene="hcaD"
                     /locus_tag="ACHKTW_000144"
     CDS             41790..42992
                     /gene="hcaD"
                     /locus_tag="ACHKTW_000144"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
                     /protein_id="HFO6461240.1"
                     /translation="MKEKTIIIVGGGQAAAMAAASLRQQGFTGELHLFSDERHLPYER
                     PPLSKSMLLEDSPQLQQVLPANWWQENNVHLHSGVTIKTLGRDTRELVLTNGESWHWD
                     QLFIATGAAARPLPLLDALGERCFTLRHAGDAARLREVLQPERSVVIVGAGTIGLELA
                     ASATQRRCKVTVIELAATVMGRNAPPPVQRYLLQRHQQAGVRILLNNAIEHVVDGEKV
                     ELTLQSGETLQADVVIYGIGISANEQLAREANLDTANGIVIDEACRTCDPAIFAGGDV
                     AITRLDNGALHRCESWENANNQAQIAAAAMLGLPLPLLPPPWFWSDQYSDNLQFIGDM
                     RGDDWLCRGNPETQKAIWFNLQNGVLIGAVTLNQGREIRPIRKWIQSGKTFDAKLLID
                     ENIALKSL"
     gene            43089..43511
                     /gene="yphA"
                     /locus_tag="ACHKTW_000145"
     CDS             43089..43511
                     /gene="yphA"
                     /locus_tag="ACHKTW_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFO6461241.1"
                     /translation="MNTLRYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(43559..44431)
                     /gene="yphB"
                     /locus_tag="ACHKTW_000146"
     CDS             complement(43559..44431)
                     /gene="yphB"
                     /locus_tag="ACHKTW_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HFO6461242.1"
                     /translation="MTIYTLSHGSLKLDVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQPAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDDHHRPEGGDLIALAPG
                     ESTTSEMSLRVEWL"
     gene            complement(44443..45537)
                     /gene="yphC"
                     /locus_tag="ACHKTW_000147"
     CDS             complement(44443..45537)
                     /gene="yphC"
                     /locus_tag="ACHKTW_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFO6461243.1"
                     /translation="MKINRIVNEGFMKTMLAAYLPGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGKGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGVMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALRSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYALMASGKCGKVVINFPD"
     gene            complement(45570..46568)
                     /gene="yphD"
                     /locus_tag="ACHKTW_000148"
     CDS             complement(45570..46568)
                     /gene="yphD"
                     /locus_tag="ACHKTW_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO6461244.1"
                     /translation="MSASSLSLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAVACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMIVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            complement(46593..48104)
                     /gene="yphE"
                     /locus_tag="ACHKTW_000149"
     CDS             complement(46593..48104)
                     /gene="yphE"
                     /locus_tag="ACHKTW_000149"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HFO6461245.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTDSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASC
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLEEYEQGEIVINGEKITRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISANGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHSPVNVDELMSAILSVH"
     gene            complement(48127..49110)
                     /gene="yphF"
                     /locus_tag="ACHKTW_000150"
     CDS             complement(48127..49110)
                     /gene="yphF"
                     /locus_tag="ACHKTW_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFO6461246.1"
                     /translation="MPKKMRTTRNLLLMGTLLGSALFARAADKEMTIGAIYLDTQGYY
                     AGVRQGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDAIILSAVSENGSS
                     RTVRRASEAGIPVICYNTCINQKGVDKYVSAYLVGDPLEFGKKLGNAAADYFIANKID
                     QPKIAVINCEAFEVCVQRRKGFEEVLKSRVPGAQIVANQEGTVLDKAISVGEKLIIST
                     PDLNAIMGESGGATLGAVKAVRNQNQAGKIAVFGSDMTTEIAQELENNQVLKAVVDIS
                     GKKMGNAVFAQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATHVDGLP"
     gene            complement(49207..52488)
                     /gene="yphG"
                     /locus_tag="ACHKTW_000151"
     CDS             complement(49207..52488)
                     /gene="yphG"
                     /locus_tag="ACHKTW_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HFO6461247.1"
                     /translation="MTPVKVWQERVEIPTYETGPQDIHPMFLENRVYQGSSGAVYPYG
                     VTDTLSEQKTLKSWQAVWLENDYIKVMILPELGGRVHRAWDKVKQRDFVYHNEVIKPA
                     LVGLLGPWISGGIEFNWPQHHRPTTFMPVDFTLEAHEDGAQTVWVGETEPMHGLQVMT
                     GFTLRPDRAALEIASRVYNGNATPRHFLWWANPAVKGGEGHQSVFPPDVTAVFDHGKR
                     AVSAFPIATGTYYKVDYSAGVDISRYKNVPVPTSYMAEKSQYDFVGAWCHDEDGGLLH
                     VANHHIAPGKKQWSWGHSEFGQAWDKSLTDNNGPYIELMTGIFADNQPDFTWLDAYEE
                     KRFEQYFLPYHSLGMVQNASRDAVIKLQRSKRGIEWGLYAISPLNGYRLAIREIGKCN
                     ALLDDAVALMPATAIQGVLHGINPERLTIELSDADGNIVLSYQEHQPQELPLPDVAKA
                     PLAAQDITSTDEAWFIGQHLEQYHHASRSPFDYYLRGVVLDPLDYRCNLALAMLEYNR
                     ADFPQAVAYATQALKRAHALNKNPQCGQASLIRASAYERQGQYQQAEEDFWRAVWSGN
                     SKAGGYYGLARLAARNGNFDAGLDFCQQSLRACPTNQEVLCLHNLLLVLSGRQDNARV
                     QREKLLRDYPLNATLWWLNWFDGRSESALAQWRGLCQGRDVNALMTAGQLINWGMPTL
                     AAEMLNALDCQRTLPLYLQASLLPKAERGELVAKAIDVFPQFVRFPNTLEEVAALESI
                     EECWFARHLLACFYYNKRSYNKAITLWQRCVEMSPEFADGWRGLAIHAWNKQHDYELA
                     ARYLDNAYQLAPQDARLLFERDLLDKLSGATPEKRLARLENNLEIALKRDDMTAELLN
                     LWHLTGQADKAADILATRKFHPWEGGEGKVTSQFILNQLLRAWQHLDAREPQQASELL
                     HAALHYPENLSEGRLPGQTDNDIWFWQAICANAQGDETEAMRCLRLAATGDRTINIHS
                     YYNDQPVDYLFWQGMALRLLGEQQTAQQLFSEMKQWAQEMAKTSIEADFFAVSQPDLL
                     SLYGDLQQQHKEKCLMVAMLASAGLGEVAQYESARAELTAINPAWPKAALFTTVMPFI
                     FNYVH"
     gene            52606..53799
                     /gene="yphH"
                     /locus_tag="ACHKTW_000152"
     CDS             52606..53799
                     /gene="yphH"
                     /locus_tag="ACHKTW_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFO6461248.1"
                     /translation="MRACINNQQIRHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPKGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDAPTPQALLSEIEKCWHRHRKLWPDRTINLALAIHGQVDPVTGVSQT
                     MPQAPWTTPVEVKYLLEEKLGIRVMVDNDCVMLALAEKWQNNSQERDFCVINVDYGIG
                     SSFVINEQIYRGSLYGSGQIGHTIVNPDGVVCDCGRYGCLETVASLSALKKQARVWLK
                     SQPVNTQLDPEKLTTAQLIAAWQSGEPWITSWVDRSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGENWLNTIIRQTGFNPFDRDEGPSVKATQIGFGQLSRAQQVLGIGYLYVEA
                     QLRQI"
     gene            complement(53863..55116)
                     /gene="glyA"
                     /locus_tag="ACHKTW_000153"
     CDS             complement(53863..55116)
                     /gene="glyA"
                     /locus_tag="ACHKTW_000153"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFO6461249.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            55444..56634
                     /gene="hmpA"
                     /locus_tag="ACHKTW_000154"
     CDS             55444..56634
                     /gene="hmpA"
                     /locus_tag="ACHKTW_000154"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008941 - nitric oxide dioxygenase NAD(P)H
                     activity [Evidence IEA];
                     GO_function: GO:0019825 - oxygen binding [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0051409 - response to nitrosative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HFO6461250.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISAGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGDVHAFADEVKELGQSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFTAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            complement(56679..57017)
                     /gene="glnB"
                     /locus_tag="ACHKTW_000155"
     CDS             complement(56679..57017)
                     /gene="glnB"
                     /locus_tag="ACHKTW_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HFO6461251.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(57078..58412)
                     /gene="glrR"
                     /locus_tag="ACHKTW_000156"
     CDS             complement(57078..58412)
                     /gene="glrR"
                     /locus_tag="ACHKTW_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HFO6461252.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDIDVRIISAT
                     HRDLPKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNQFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            complement(58402..59115)
                     /gene="qseG"
                     /locus_tag="ACHKTW_000157"
     CDS             complement(58402..59115)
                     /gene="qseG"
                     /locus_tag="ACHKTW_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HFO6461253.1"
                     /translation="MRHIFQRLMPRRLWLAGLPCLALLGCVQSHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIDALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYGKLQQASDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHEGEKPAPSTHEVTPDEP"
     gene            complement(59280..60707)
                     /gene="qseE"
                     /locus_tag="ACHKTW_000158"
     CDS             complement(59280..60707)
                     /gene="qseE"
                     /locus_tag="ACHKTW_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HFO6461254.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFSGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSRNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSL
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            complement(61265..65152)
                     /gene="purL"
                     /locus_tag="ACHKTW_000159"
                     /gene_synonym="purI"
     CDS             complement(61265..65152)
                     /gene="purL"
                     /locus_tag="ACHKTW_000159"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HFO6461255.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFALDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWVIDGEQQPKSLFKMIKNTFETTPDYVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYDFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALAREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLSTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVAQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGINADIASLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFHRAGFDAIDVHMSDLLAGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKVLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            65410..66966
                     /gene="mltF"
                     /locus_tag="ACHKTW_000160"
     CDS             65410..66966
                     /gene="mltF"
                     /locus_tag="ACHKTW_000160"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HFO6461256.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKETKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            complement(66963..67466)
                     /gene="tadA"
                     /locus_tag="ACHKTW_000161"
     CDS             complement(66963..67466)
                     /gene="tadA"
                     /locus_tag="ACHKTW_000161"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFO6461257.1"
                     /translation="MSEVEFSHEYWMRHALTLAKRAWDEREVPVGAVLVHNNRVIGEG
                     WNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCAGAMIHSRIGRV
                     VFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFFRMRRQEIKAQK
                     KAQSSTD"
     gene            complement(67524..68159)
                     /gene="yfhb"
                     /locus_tag="ACHKTW_000162"
     CDS             complement(67524..68159)
                     /gene="yfhb"
                     /locus_tag="ACHKTW_000162"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HFO6461258.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQTLQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            68368..69216
                     /gene="yfhH"
                     /locus_tag="ACHKTW_000163"
     CDS             68368..69216
                     /gene="yfhH"
                     /locus_tag="ACHKTW_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFO6461259.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAAVRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            69272..69532
                     /gene="yfhL"
                     /locus_tag="ACHKTW_000164"
     CDS             69272..69532
                     /gene="yfhL"
                     /locus_tag="ACHKTW_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HFO6461260.1"
                     /translation="MALLITKKCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPAHVETEEQLWDKFVLMHHADKI"
     gene            complement(69726..69806)
                     /gene="shoB"
                     /locus_tag="ACHKTW_000165"
     CDS             complement(69726..69806)
                     /gene="shoB"
                     /locus_tag="ACHKTW_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HFO6461261.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
     gene            complement(70226..70606)
                     /gene="acpS"
                     /locus_tag="ACHKTW_000166"
     CDS             complement(70226..70606)
                     /gene="acpS"
                     /locus_tag="ACHKTW_000166"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFO6461262.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            complement(70606..71337)
                     /gene="pdxJ"
                     /locus_tag="ACHKTW_000167"
     CDS             complement(70606..71337)
                     /gene="pdxJ"
                     /locus_tag="ACHKTW_000167"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HFO6461263.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQRDKMRDACKRLADAGIQVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELARIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            complement(71349..72077)
                     /gene="recO"
                     /locus_tag="ACHKTW_000168"
     CDS             complement(71349..72077)
                     /gene="recO"
                     /locus_tag="ACHKTW_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFO6461264.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGATGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDD
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            complement(72089..72994)
                     /gene="era"
                     /locus_tag="ACHKTW_000169"
     CDS             complement(72089..72994)
                     /gene="era"
                     /locus_tag="ACHKTW_000169"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFO6461265.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLRDGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGYDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            complement(72991..73671)
                     /gene="rnc"
                     /locus_tag="ACHKTW_000170"
     CDS             complement(72991..73671)
                     /gene="rnc"
                     /locus_tag="ACHKTW_000170"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFO6461266.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            complement(73944..74918)
                     /gene="lepB"
                     /locus_tag="ACHKTW_000171"
     CDS             complement(73944..74918)
                     /gene="lepB"
                     /locus_tag="ACHKTW_000171"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFO6461267.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            complement(74934..76733)
                     /gene="lepA"
                     /locus_tag="ACHKTW_000172"
     CDS             complement(74934..76733)
                     /gene="lepA"
                     /locus_tag="ACHKTW_000172"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFO6461268.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            complement(76931..77410)
                     /gene="rseC"
                     /locus_tag="ACHKTW_000173"
     CDS             complement(76931..77410)
                     /gene="rseC"
                     /locus_tag="ACHKTW_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HFO6461269.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAVLGGIGGFLIARGYSRKFAARAEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            complement(77407..78363)
                     /gene="rseB"
                     /locus_tag="ACHKTW_000174"
     CDS             complement(77407..78363)
                     /gene="rseB"
                     /locus_tag="ACHKTW_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HFO6461270.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            complement(78363..79013)
                     /gene="rseA"
                     /locus_tag="ACHKTW_000175"
     CDS             complement(78363..79013)
                     /gene="rseA"
                     /locus_tag="ACHKTW_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HFO6461271.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQPATLIPEAQPAPHQWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            complement(79046..79621)
                     /gene="rpoE"
                     /locus_tag="ACHKTW_000176"
     CDS             complement(79046..79621)
                     /gene="rpoE"
                     /locus_tag="ACHKTW_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HFO6461272.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            complement(79618..79773)
                     /gene="rseD"
                     /locus_tag="ACHKTW_000177"
     CDS             complement(79618..79773)
                     /gene="rseD"
                     /locus_tag="ACHKTW_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HFO6461273.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            80029..81651
                     /gene="nadB"
                     /locus_tag="ACHKTW_000178"
     CDS             80029..81651
                     /gene="nadB"
                     /locus_tag="ACHKTW_000178"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HFO6461274.1"
                     /translation="MNTLPEHSCDVLIIGSGAAGLSLALRLADQHQVIVLSKGPVTEG
                     STFYAQGGIAAVFDETDSIDSHVEDTLIAGAGICDRHAVEFVASNARSCVQWLIDQGV
                     LFDTHVQPNGEESYHLTREGGHSHRRIIHAADATGREVQSTLVSKAQNHPNIRVLERS
                     NAVDLIVSDKIGLPGTRRVVGAWVWNRNKETVETCHAKAVVLATGGASKVYQYTTNPD
                     ISSGDGIAMAWRAGCRVANLEFNQFHPTALYHPQARNFLLTEALRGEGAYLKRPDGTR
                     FMPDFDERGELAPRDIVARAIDHEMKRLGADCMFLDISHKPADFIRQHFPMIYEKLLG
                     LGIDLTQEPVPIVPAAHYTCGGVMVDDHGRTDVEGLYAIGEVSYTGLHGANRMASNSL
                     LECLVYGWSAAEDITRRMPDAHGVSTLPPWDESRVENPDERVVIQHNWHELRLFMWDY
                     VGIVRTTKRLERALRRITMLQQEIDEYYAHFRVSNNLLELRNLVQVAELIVRCAMMRK
                     ESRGLHFTLDYPELLTHSGPSILSPGNHYINR"
     gene            complement(81636..82373)
                     /gene="trmN"
                     /locus_tag="ACHKTW_000179"
     CDS             complement(81636..82373)
                     /gene="trmN"
                     /locus_tag="ACHKTW_000179"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HFO6461275.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWITQQTVRFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            82505..83839
                     /gene="srmB"
                     /locus_tag="ACHKTW_000180"
     CDS             82505..83839
                     /gene="srmB"
                     /locus_tag="ACHKTW_000180"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0000027 - ribosomal large subunit assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HFO6461276.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            complement(84048..84929)
                     /gene="yfiE"
                     /locus_tag="ACHKTW_000181"
     CDS             complement(84048..84929)
                     /gene="yfiE"
                     /locus_tag="ACHKTW_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO6461277.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQSLNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     LSIESIKRCVAANIGVSYLPRFAVEKELESGELIELPFGEQSQTITAMCAHHAGKAVS
                     PAMHTFIQCVEESFVAG"
     gene            85032..85619
                     /gene="eamB"
                     /locus_tag="ACHKTW_000182"
     CDS             85032..85619
                     /gene="eamB"
                     /locus_tag="ACHKTW_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HFO6461278.1"
                     /translation="MTPPLLSAFWTYTLITAMTPGPNNILALSSATSHGFRQSTRVLA
                     GMTLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLIYCAVRIFY"
     gene            complement(85675..86058)
                     /gene="grcA"
                     /locus_tag="ACHKTW_000183"
     CDS             complement(85675..86058)
                     /gene="grcA"
                     /locus_tag="ACHKTW_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HFO6461279.1"
                     /translation="MITGIQITKAANDDLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            86363..87052
                     /gene="ung"
                     /locus_tag="ACHKTW_000184"
     CDS             86363..87052
                     /gene="ung"
                     /locus_tag="ACHKTW_000184"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFO6461280.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIAIPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAESE"
     gene            complement(87100..88137)
                     /gene="yfiF"
                     /locus_tag="ACHKTW_000185"
     CDS             complement(87100..88137)
                     /gene="yfiF"
                     /locus_tag="ACHKTW_000185"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HFO6461281.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVSQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            88344..88763
                     /gene="trxC"
                     /locus_tag="ACHKTW_000186"
     CDS             88344..88763
                     /gene="trxC"
                     /locus_tag="ACHKTW_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HFO6461282.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNRQVVDMLNGAVPKAPFDSWLNESL"
     gene            88832..89530
                     /gene="tapT"
                     /locus_tag="ACHKTW_000187"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             88832..89530
                     /gene="tapT"
                     /locus_tag="ACHKTW_000187"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HFO6461283.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAAGQYCTAEVAIALLDMAGDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            89562..92222
                     /gene="pat"
                     /locus_tag="ACHKTW_000188"
     CDS             89562..92222
                     /gene="pat"
                     /locus_tag="ACHKTW_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HFO6461284.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSHNGKLATLSEETCQKLRDALPGHV
                     AISNPLDLRDDASSEHYVKTLDILLHSQDFDALMVIHSPSAAAPATESAQELIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVAFPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDISPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRDHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLAQREES"
     gene            92336..93691
                     /gene="pssA"
                     /locus_tag="ACHKTW_000189"
     CDS             92336..93691
                     /gene="pssA"
                     /locus_tag="ACHKTW_000189"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HFO6461285.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENPGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIREHTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            93737..94060
                     /gene="yfiM"
                     /locus_tag="ACHKTW_000190"
     CDS             93737..94060
                     /gene="yfiM"
                     /locus_tag="ACHKTW_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HFO6461286.1"
                     /translation="MRILFVCSLLLLSGCSHMANDSWSGQDKAQHFIASAMLSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDLAWDVAGASTGYTVW
                     QLTRH"
     gene            complement(94057..95355)
                     /gene="kgtP"
                     /locus_tag="ACHKTW_000191"
     CDS             complement(94057..95355)
                     /gene="kgtP"
                     /locus_tag="ACHKTW_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HFO6461287.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
BASE COUNT        22787 a        26075 c        24038 g        22715 t
ORIGIN      
        1 taacaaggaa tagtgatgaa atttaaaaaa tgtcttctgc ctgtggcaat gttagcgtca
       61 ttcactctgg caggatgcca gtcaaatgct gacgatcatg ccgccgatgt ttatcaaacc
      121 gatcaactga ataccaaaca agaaactaaa accgttaata ttatttccat tcttcccgca
      181 aaagttgccg tagacaactc ccaaaataaa cggaacgcac aagccttcgg cgcgcttatt
      241 ggcgcagtcg ctggcggtgt tatcggccac aacgtcggtt ctggcagcaa ttccggaacg
      301 acggcagggg cagttggcgg cggagctgta ggcgcggcag tgggttctat ggtgaatgat
      361 aaaaccttag tggaaggtgt ttctttaaca tataaggaag gcaccaaagt gtatacctct
      421 acccaggtgg gtaaagagtg ccagtttacg acaggtttag ccgttgttat taccacaacg
      481 tataacgaaa cgcgtattca gccaaatact aaatgtcctg aaaagagcta ataatcagga
      541 ggagtcatga agaaagtttt tctttgcgcc atcttggcct ccttaagcta tccggctatc
      601 gcctcatcat tgcaggatca actctctgct gtagcagaag ctgaacagca aggtaaaaat
      661 gaagagcaaa ggcagcatga cgaatgggtc gcggagcgca acagggaaat ccagcaagag
      721 aagcaacgtc gcgcaaatgc ccaggccgcc gctaacaaaa gagcggcaac ggcagcggca
      781 aataagaaag ctcgtcagga taaactggac gccgaagcct ctgcggacaa aaaacgcgat
      841 caaagttatg aagatgagct acgcagctta gagattcaga aacaaaaact ggcgctggca
      901 aaagaagaag cccgcgttaa gcgagaaaac gaatttatcg atcaggaact gaagcacaaa
      961 gctgcgcaaa ccgatgtggt gcaatctgaa gctgacgcca acagaaatat gactgaaggc
     1021 ggtcgcgatc tgatgaaaag cgtgggcaaa gcagaagaga acaaatcgga cagctggttt
     1081 aattaatcga tgttagtaac ttcaagccta tgattcttga agataaaaaa ccctctgtag
     1141 taacagaggg ttttgttcat tcatagtgca gggattaaaa tcattcccac tcaatggtag
     1201 ctggcggctt gccgctgatg tcatacacca cgcgggaaat accgttcact tcattgataa
     1261 tgcggttgga aacgcgaccg aggaaatcgt acggcagatg cgcccagtgt gcggtcataa
     1321 agtcgatggt ttcgacagca cgcagagaga caacccagtc atacttacga ccatcgccca
     1381 ttacgccaac ggaacgtacc ggcaggaaca cagtgaacgc ctggctgact ttgtcgtaca
     1441 ggtccgcttt acgcagttct tcaatgaaga tggcgtcagc acggcgcagc aggtcacagt
     1501 actctttctt cacttcaccc agcacacgaa cgccaaggcc tggtcccggg aacgggtgac
     1561 ggtacagcat gtcgtacggc aggcccagct ccagaccaat cttacgcact tcgtctttga
     1621 acagctcttt cagcggttca accaggccca tcttcatctc tttcggcagg ccgcccacgt
     1681 tgtggtgaga tttgatgacg tgtgctttac cggttgcaga agccgcagat tcgataacgt
     1741 cagggtagat ggtgccctgc gccagccact tcacgtcttc cagtttcagc gcttcttcat
     1801 cgaatacttc aacgaaaacg cgcccgatga ttttacgttt tgcttccgga tcgttttcgc
     1861 cagccagcgc tgacaggaag cgatcttctg ccggtacgtg aacaatgtta agaccaaagt
     1921 gatcgccaaa catatccaga acctgctctg cttcgttgag acgcagcagg ccgttgtcga
     1981 cgaatacgca agtcaggttt ttaccgatag cgcggtgcag cagcattgcg gttacggagg
     2041 aatccacacc accagagagg ccgaggatga ctttatcgtc gcctacctgc tcgcggatgc
     2101 gagctacagc atcgtcgata attttcgctg gcgtccacag ggcttcacac tggcagatat
     2161 cacgcacaaa acgctccagc atgcgcatac cctggcgggt gtgagtcact tccgggtgga
     2221 actgtacgcc atagaagcgt ttttcttcgt tagccataat ggcaaacggg cagctttcgg
     2281 tgctggctac ggtgacgaag tcggacggga tagcggtaac tttatcgccg tggctcatcc
     2341 agacatcgag cagcggttta ccgtctgcgg tcagcgcatc ttcgataccg cgaaccagtg
     2401 cgctgtcgtt tacgacttca acctgcgcgt agccaaattc acgttcgtta gaggcttcaa
     2461 cgtgaccgcc caactgcatt gccatggttt gcatgccata gcaaacgccg aataccggta
     2521 cgcctgcttc aaagacatac tgcggcgcac gcgggctgtt ttcttcggtg gtgctttccg
     2581 ggccgccgga aagaataatg ccgcttggat tgaagtcacg aatttgtgct tctgtcacat
     2641 cccacgccca cagttcgcag taaacaccca gctcacgcac gcggcgcgca accagttgag
     2701 tgtactgaga accgaagtcc agaatgagga tgcgatgctt atgaatgttt tccgtcattg
     2761 acgcttattc cgaggcaagt gaaacagata atataaatcg cccgacatga agtcgggcga
     2821 agagaatcag gagcccagac ggtagttcgg ggactcttta gtaatggtca cgtcgtgaac
     2881 gtggctttcc tgaatgcccg caccgctgat acgtacaaac tccgctttag tacgcagttc
     2941 gtcgatagta ccacagccgg tcagacccat acaggagcgc aggccgccca tctgctggtg
     3001 aatgatctct ttcaggcgac ctttataagc tacgcgacct tcgatacctt ccggcaccag
     3061 tttgtcggca gcgttatcgc tctggaaata acggtcagag gaacctttgg acatcgcgcc
     3121 cagggaaccc ataccacggt aagatttgta agaacggccc tggtagagtt cgatttcacc
     3181 cggagattct tcagtacccg ccagcatgga acccaccatt accgcgcttg cgccagcggc
     3241 gatagctttg gcgatgtcgc cggagaagcg aataccacca tctgcgataa ccggaatacc
     3301 ggtgccttcc agggcttcaa ctgcgtcagc aacggcagtg atctgcggaa cacctacgcc
     3361 agtaacgata cgagtcgtac agatagaacc agggccgata ccgactttaa ccgcgctgca
     3421 accagcttct gccaaagcgc gtgcacctgc agctgttgcc acgttgccgc cgatgatttg
     3481 cagatccgga tatttagcac gggtttcacg gatacgctgc aaaacgcctt ctgagtgacc
     3541 gtgggaggag tcgatcagca gaacgtcaac gccagcggca accagcgcgt caacacgctc
     3601 ttcgttaccc gcacctgcgc caactgcagc accaacacgc agacggcctt gctcgtcttt
     3661 acaggcgttc ggtttacgtt ccgctttctg gaagtctttc acggtgatca tgccgatcag
     3721 gtggaattcg tcatcaacca ccagcgcttt ttcaacgcgt ttttcgtgca tttttgccag
     3781 caccacttca cgggcttcac cttcacgcac ggtgaccaga cgctctttcg gcgtcatgta
     3841 aacgctaacc ggctggttca ggtcggtaac aaaacgcacg tcacgaccgg tgataatacc
     3901 caccagttcg ttttcttcgg taacgaccgg atagcccgca aaaccgttac gctcagtcag
     3961 ttctttcact tcgcgcagcg tcgtggttgg cagaacagtc tgcggatcag tcaccacacc
     4021 agattcgtgt tttttcacac ggcgaacttc ttctgcctgg cgttcaatgg acatgttttt
     4081 gtggataaag ccgataccgc cttcctgagc cagagcaata gccaggcgcg cttccgttac
     4141 ggtatccatt gctgcggaaa gcatagggat attcagacga atagttttcg tcagctgggt
     4201 gctgaggtca gcagtattcg gcagaacggt agaatgagca ggaacgagga gaacgtcgtc
     4261 aaacgtcaga gcttctttag cgatacgtag catgggcaat atctcgacca gagtggttaa
     4321 taaatattgc cgcggcatta tacagagcgt aaccgattgc atctacccct ttttgcaaaa
     4381 aatgcttgct atccccgaag ggcgggttac tatcgactga ataacctgct gatttagaat
     4441 ttgatctcgc tcacatgtta ccttctcaat cccctgcaat ttttaccgtt agtcgcctga
     4501 atcaaacggt tcgtctgctg cttgagcatg agatgggaca ggtttggatc agcggcgaaa
     4561 tctctaattt cacacaaccg gcttccggtc actggtactt tacactcaaa gacgacaccg
     4621 cccaggtacg ctgcgcgatg ttccgcaaca gcaaccgccg ggtgaccttc cgtccacagc
     4681 atggacaaca agttttagtt cgcgccaata ttacgctcta cgagccgcgc ggtgactacc
     4741 agataatcgt tgagagtatg cagccggccg gtgaagggct gctgcaacag aagtacgaac
     4801 agctcaaagc gaagttgcag gctgaaggtt tgttcgatca gcaatacaaa aaaccacttc
     4861 cctcccctgc gcattgcgtt ggtgtgatca cctcaaaaac cggtgctgcg ctacatgata
     4921 ttttgcatgt gttaaaacgt cgcgatcctt ctctaccggt gatcatctac cccaccgccg
     4981 ttcagggcga tgacgcaccg gggcaaattg ttcgcgccat tgaactggcg aatcagcgca
     5041 atgagtgcga cgtgttgatc gttgggcgcg gcggcggttc gctggaagat ttatggagtt
     5101 ttaacgacga acgcgtagcg cgggcgattt ttgccagccg cattccggtc gtcagcgccg
     5161 tcgggcatga gacggatgtg accattgccg attttgttgc cgatctgcgt gcgccaacgc
     5221 cgtctgccgc cgctgaagta gtgagccgta atcagcaaga gttactgcgc caggtgcaat
     5281 cgacccgtca acggctggag atggcgatgg attattatct cgccaaccgc acgcgtcgct
     5341 ttacgcagat ccatcaccga ttacagcaac agcatccgca gctccggctg gcacgccagc
     5401 aaaccatgct tgagcgcctg caaaagcgga tgagctttgc gctggaaaat caacttaagc
     5461 gtaccgggca acagcagcag cggttaacac agcggctgaa tcagcaaaat ccacagccga
     5521 agattcatcg cgcgcaaacg cgcattcagc aactggaata tcgtttagca gaaaccctgc
     5581 gcgtacagct tagcgccacg cgtgaacgtt tcggtaatgc agtaacgcac ctcgaagccg
     5641 taagcccact gtcaacgctg gcgcgtggat acagcgttac tactgctact gacggcaatg
     5701 tactgaaaaa agtgaagcaa gttaaagcgg gtgaaatgct aaccacacgt ctggaagacg
     5761 gctggataga aagtgaagtt aaaaacatcc agccagtaaa aaaatcgcgt aaaaaggtgc
     5821 attaagccag cacgaattca acgcgttttt tcgatatcaa gccatgcccg tgctgacaga
     5881 agtaatctac tgcaccgcag gcttttaaca cctgcaacgg ttgatggcag tctggacaaa
     5941 gagctttcat ttcaataatt tccccgcagg atgggcaacg cgcatggtcg ttatcctgat
     6001 caagcacatg ctgacattgt ggacaatgca gttccatatg aattcctccg gtagccatcc
     6061 ctctacattc atagagggaa tggcagataa aatacttacg gataattatt tatttttctt
     6121 gatgtgcttc atcagacgct tacgtttacg catctgggtt ggcgtcaggg tgttacgctt
     6181 gttcgcatac gggttttccc cttctttgaa ctgaatacga atcggcgatc ccattacgtc
     6241 cagcgatttg cggaagtagt tcatcaagta gcgcttgtag gaatcaggca ggtctttcac
     6301 ctgattaccg tgaatcacca caatcggcgg gttataacca ccagcgtggg catatttcag
     6361 cttcacacga cgaccgcgta ccagcggcgg ttggtgatct tcaacagcca tcgtcatgat
     6421 gcgcgtcagc atagaggtcc ccacacgacg ggtggagctg tcatacgctt cacgtactga
     6481 ttcaaacaag ttaccaacac cactgccgtg caaggcagag ataaagtgca cacgagcaaa
     6541 atcgataaag cccagacgga agtccagcgt ttctttcacc tgctctttca cttcctggct
     6601 caggccatcc cacttattca ccacaatgac aagtgagcgc ccactattga gaataaagcc
     6661 cagcagcgag agatcctgat cggaaatacc ttcgcgcgca tcaatcacta acatcaccac
     6721 gttggcatct tcaatggcct gcaacgtttt gattacggag aatttctcta cagcatcggt
     6781 gattttgccg cgtttacgta cgccagcggt gtcaatgagc acatactcac gtccatcgcg
     6841 ttccattgga atgtagatac tgtcacgcgt cgtgccaggc atgtcataaa caacaacgcg
     6901 ctcttcacca agaatacggt tagtgagtgt agacttacct acgttcggac gacccacaat
     6961 cgccagtttg atcggcagac tttgcgggtc gaagtcgtct tcctcttctt cttcgccgtt
     7021 ctcttccgct tcaaattgcg cccagtattc agcgtcttcg tcgacttcct cttgcggtgc
     7081 gagatcttcc atccacggca gcagcacatg ctccagcaga cttaatacgc cacgaccgtg
     7141 agacgcggcg atcgggtaga tttcacctaa accaagcgag tagaaatcaa ccactgcctg
     7201 atcgggatcc agaccgtcag ttttgtttgc caccaggaag gtcggttttt cacgggagcg
     7261 cagatgtttg gcaatcgctt catctgccgg catcaggccc gcgcgcgcat ccaccataaa
     7321 cagtacgacg tccgcttctt caatcgccag cagcgactgt tccgccatgc gggtttctac
     7381 accgtcttct gtgccatcaa tcccgccggt atcaatacag ataaactcac ggccttcaat
     7441 ttccgcacga ccgtacttac ggtcacgagt cagacccggg aaatccgcaa ccagcgcatc
     7501 tcgggtgcga gttagacggt taaataacgt ggattttcct acgttagggc gcccgacaag
     7561 cgcgaccaca ggtaccatgt ttaaagcctc atttttataa atcatcagac aacgcacgct
     7621 atattcgcgt cgttgttaaa aacaggaaaa cggcccctgt ccaggagccg ttttcaaagt
     7681 gaacgacaga gacgattaac gtgtaataga gtacacggtt ccgtcttttg cctggatcag
     7741 cagtttgccg tcagcggcaa ccggttcagt ctggaaaccg gaactatcaa ctttttgctg
     7801 ggcaacgaaa cgaccatctt cgacgttaat ccagtgcaga taaccttcac tgtcaccgac
     7861 caccaggttg ccattataca gcaccggaga agtcagcagg cgatgcagca gatcgctttg
     7921 tgcccacagc gtaacgccgc catcaatggt caacgccatc acccggtcat tttgatcgac
     7981 cagatagatg cgattgccgt cgacgatgaa atcattcacc gaacccagtt cgcgtttcca
     8041 cataatctga ccactgcgca gatcaagcgc cgtcaggtta ccattatagg ccagcgcgaa
     8101 aacaacgccg ttaacaacga cgggagtcgt gtcaacatcg ctcagacggt caatttcggt
     8161 agaaccggtc gcctgggaaa tacgctgctg ccaaatcatc tggccctgtt ccatcagcac
     8221 tgcgctgacg cgaccattat cgccccccac gacggccgca ccaaaagccg ttgccggcgc
     8281 agactcgcca cgcaaagaga gcgaaggcat atcgaggtta actgtccatt tgacagcgcc
     8341 gtcagcttcg ttcaacgctt gtaactgacc gttactggtg tggattaaca ccagaccgtc
     8401 gctgaccacc gggcgcgaaa gtgcttcacc cgcgacttta gtttgccatg ccacagtacc
     8461 atcgctggta ttcagcgcgt aaacctgcgc cttttcgctg ccaatgtaga catgcccacc
     8521 agacacggtc acaccgccag aaagtaatgc aggctcttta gagaaccagc catctttctc
     8581 ggccaagctg acagaccaga tttctttgcc atcatccgca ttcagcgctt ttaccaaacc
     8641 agcgcggtcc gctgcataga caacgttgtc cgccagtgcc ggatgaagat tggaatagaa
     8701 gttgccaatg ccgctaccaa cggaagtact ccacgccgtg gtcggcgtaa actggttttc
     8761 aacggttggc aatggggaca tctttaccac atcttcttcg ctgttaaaca gcgaacagcc
     8821 gcttaaaagg gtaacggaaa gcagtcctgg cagcagtaat ttacgcaatt gcatcgggtc
     8881 cctctcagat ggacaaatta ttaattttca tctgcatcat ttcgctcagt gccggagtaa
     8941 catcgctttt cacgcctgct tcccatgcac tacgcgcacc ttgcttatca cctttgctca
     9001 gcaatgcttc accacgcagg tcggcaacaa tggcagccca cccttcacct ttgatggtat
     9061 caagggtttt cagcgcggca tcagcctgct tgagctgtac ctgaacgcga gcaagacgca
     9121 gatttatcac ggctttgaga ttttcatcgc tcgtgtctgc cagcccctgt tgtaactggg
     9181 cggcagcttt ctctagttca tttttgtcaa caaattgctg cgcaagttcc aaagaagcca
     9241 gcgcaccata agtattttta ttttcagcag caaatttttc cgccgccggg atgctatccg
     9301 gtttgccttc gctcactgcg gtaaccgcat tttgataggc aagagaagcg gagcgtgcag
     9361 aatcaacctg atggctgttc cagtagcgcc agccaatcag tgcgccaacg cccaaaatca
     9421 ccccaacagc cagtgctttg ccattttcag caaaaaagcg tttaaccgct tctacctggt
     9481 cgttttcgtt ctcgtaaatt tccacgctgt ccttctcctt ccttaaccca gtaacgtgcg
     9541 caaatgcgcg gctacgctat cctgcgcaac tgccgtttgc tcaccagagc gcaaatcctt
     9601 cactactgct gtgccgttag ccacttcaga ctcacccagc accacagcaa cgcgggcacc
     9661 ccatttatca gcacgggcaa actgtttctt aaagttgccg ccgccgtggt tggtcatcaa
     9721 tttcacgccc ggtaattcat cacgcagacg ctcagctaat gccatagccg cagattgtgt
     9781 atcagcacct gaagccacca ggtatatatc gacaacagga tcggctttaa attccggatt
     9841 aacggcctgt actaacaata caagacgttc gaggcccatc gcaaaaccga cagccggtgt
     9901 tgcacgaccg cccagttgtt ccacaagacc gtcataacga ccgcctgcac acacggtgcc
     9961 ctgggagccg agactgttag tcacccactc gaaaacggta cggttatagt aatccagacc
    10021 acgcaccaga cgctggttta cggtgtaagc gatccccgcg ctctcaagca gtttgcacag
    10081 accggcaaaa tgctcacgag attcctcgtc cagataatca cctaatgccg gagcgtcgtt
    10141 gagaagcgcc tgcacttccg gattttttga atccagcacg cgcagcgggt tagtgtacat
    10201 gcggcgtttg cagtcttcgt ccagcttttc tttatgctgc tcaaggaatg ccaccagcgc
    10261 atcgcggtaa ttggcgcgtg cttccagcga accgatagaa ttcagctcaa gagttacgtg
    10321 ttcggaaata cccagcgcgc gccaccagcg ggcggtgagc ataatcagtt cagcgtcgat
    10381 atccggacct tgcagaccga aaacttcgca gcccaactga tggaactgac gataacgccc
    10441 tttctgcgga cgctcgtgac ggaacatcgg cccgatatac cacagacgct gttcctgatt
    10501 gtacagaaga ccatgctcga tgccggcgcg tacacagccc gccgtccctt cagggcgcag
    10561 agtcaggctg tcgccattgc gatcctcaaa ggtgtacatc tctttttcaa ccacgtcggt
    10621 gacttcacca atcgcacgtt tgaatagcgg ggtctgctct acaatcggca agcggatttc
    10681 actgtaaccg tagctgccga gcacgttttt cagtgtgcct tcaatgcgct gccagatggc
    10741 cgtttcgcca ggcaggtaat cgttcatgcc gcgaatggct tgaatgtttt ttgccacgtt
    10801 tattctcttt ctgaatataa aaatgaaccc tcaacgcttc cctcaatgtt tcgggagcta
    10861 tgcgggttca atcatacacg ggaagcgagg cgcttcccat cacgttatta tttttcaacc
    10921 tgctgaacgt caattcgacg cgcttcgtcc agctgactgg ctttcgcacg aatgcgtgct
    10981 tccagctggt cgatcatatc gttgttgtcc agacggtctt tgcgcacgcc atcttcatag
    11041 aggccgcttt tcttgttgcc gccggtgacg ccgagtgtag aaaccagcgc ctcacctggg
    11101 ccattcacca cgcagccgat aatcgaaacg tccatcggag tgatgatatc ttccaggcgt
    11161 tgctccagcg cgttaaccgt accgataaca tcaaattcct gacgcgaaca ggtcgggcag
    11221 gcgatgaagt tgatcccgcg cgaacggata cgcagcgatt tcaaaatatc gaaaccgact
    11281 ttgatctctt cgaccggatc ggccgccagt gatacgcgca gcgtgtcgcc gatgccttca
    11341 gacagcagca gacctaaacc aatggcggat tttactgccc cgctgcgtgc accaccggct
    11401 tcggtgatcc ccagatgcag cggctgatcg atctgttttg ccagcaaacg ataagactca
    11461 acagcgagga agacgtcaga cgctttcacg ctgactttga actgatcgaa gttcaggcga
    11521 tcgagatgat caacatggcg catggcggat tccagcaacg cctgcggcgt cggttcgcca
    11581 tacttttctt gcagatcttt ttccagcgat ccggcgttaa cgccaatacg gatcggaatg
    11641 tttttatcgc gcgcacagtc aaccaccatg cgaatacgct cttcattacc gatattgcca
    11701 gggttaatac gcagacaatc gacgccgtat tccgctactt tcagtgcaat gcgatagtcg
    11761 aagtggatgt cagccaccag cggcacgtta acctgctgtt tgatgagttt gaacgcttct
    11821 gccgcgtcca tcgtcggtac ggagacacgg acgatatcag cgccaacgcg ttccagcgcc
    11881 ttgatttgat tgaccgttgc ttcgacgtct gtcgtacgcg tattggtcat ggactgtacg
    11941 gcgatgggag caccatcgcc aatcggcaca ttcccaacgt aaatacgtgt tgattttcta
    12001 cgttgaattg gagcctggtt atgcatgaaa aatctcccgc gttacccgtc tgttactgcg
    12061 ccggtgattg ttcggcattg agggtcagac gcgcaacctg gttagttctg ataaaacgac
    12121 tcagatcgac aggtttccct tgatactgga tctgtactgc ggctggcgca ccaattttca
    12181 gtttgtatgg tgcctggccg gttaagttca aattaccgtc tttacgctgc ataccgctaa
    12241 acaatttttt accggtagca tcagtaacct ccagccagca atcggcagta aagttcatca
    12301 ccagcgcatt cggatcagcc gccggcgtag tcacgcctgc ctgatcggtt ggcaacggcg
    12361 cagcgccatc tggcgttgtt gttgccgttg gtgccggggt cgcggcggta tcaacatttg
    12421 cctgcgaagg cgaaacaacc gcattctgtt gcggatcaac agctggtgct ggcgcagtta
    12481 cggcaggtgt ttgcgtgttg gttgcggtag tatccacaga cgctggcggc gtggacgttg
    12541 tagccgggtc tgtagttgtc gacgtattta acggaacact ctgcccctgc tcgctattac
    12601 tgctcagttc cgccgaagat tgatcggcca tagtggtgat ctcttcctgc tgagctttgt
    12661 ggtcttgcca ccaccaggca ccgctcaggc cgataaccac aaacaacacc agccaggtga
    12721 aggtcatcag ccagccgtca cgttttttgc ggcgtttacc gagggaaaaa ctctgcatag
    12781 gcgcaacttt tgcagcccgt agtggagcct gcttttccag ccctggcagc agttcttctt
    12841 ctggaatatg taccagacgc gcataagagc ggatatatcc gcgcaggaat gttgaagcaa
    12901 gatcggcggg tgccttatct tcttcaatgt cgcgtaccgt ggaaaccttc aggcaaagtc
    12961 gctcggcaac ggcctgctga ctaagtccta gttgttcgcg agcattacgc aggcgagcgc
    13021 cggtagtaag tgcttcattt tggtcgtgcg tggcttcagt attcattcgc tgcaggtacg
    13081 tttaaatgag aattgagatg ccggtgaacc atcatgctca cccacaccgc gaaacatccg
    13141 ttaagttaac cgttatcata cagtataaga ccgtctgtgt gctcgtgaca aagcccacat
    13201 tcactaaggc taacttactg ttgcatcgtt acatactgcc ttaaagtcag caaaaacgca
    13261 ccgttaatat tgaccagaca attgcaactt aatacttcat tcataaaaga ttacgccacg
    13321 gtacataaag taaccgtggc gtaatggcta tcagaccgct ttaatgtcga tggcttcacc
    13381 ctgcatccgt ttacgcaggg tacgtttcgt acggtcgata acatcgcccg ccaactgacc
    13441 acaggcagca tcgatatcat caccacgggt tttacgcaca atagtggtga aaccgtagct
    13501 catcagcact tttgagaaac ggtcgatacg gctgttcgag ctgcgtccat acggcgcgcc
    13561 cgggaagggg ttccacggga tcaggttgat cttacacggc gtatctttca gcagttccgc
    13621 cagttggtgc gcgtgttcag tgccgtcgtt aacgtggtca agcatcacat actcaatagt
    13681 gacacggccc tgattggcgt tggatttctc cagataacgg cgcaccgcag caaggaacgt
    13741 ttcgatattg tactttttgt tgatcggcac aatttcgtca cgaatttcgt cgttcggcgc
    13801 gtgcagggaa attgccagtg caacgtcgat catatcgccc agtttatcca gcgccggaac
    13861 tacaccggaa gtagaaagcg tgacgcgacg tttagacagg ccaaaaccga aatcatcaag
    13921 catgatttcc atcgccggaa cgacgttgtt caggttgagc agcggctcgc ccatgcccat
    13981 catcactacg ttagtgattg ggcgctgacc ggtgactttt gctgcgccga cgattttcgc
    14041 cgcacgccac acctggccaa taatttccga tacccgcagg ttgcggttaa agccctgctg
    14101 ggcggtggaa cagaatttac actccagcgc acaccccacc tgcgaagaga cgcagagcgt
    14161 ggcacggtcg tcttccggga tatacaccgt ttcgacgcgc tgatcgccaa cggcgatcgc
    14221 ccatttaatg gtgccgtcgg atgaacgctg ttcttcaacc acttccggtg cgcggatttc
    14281 cgccacctct ttcagtttgc cgcgcaacac tttgttgatg tcggtcatct catcaaagtt
    14341 gtcgcagcaa tagtgataca tccacttcat cacctgatcg gcgcggaagg gtttttcacc
    14401 taaatcttta aaaaactccc gcatctgctg acggttgaga tccagcaggt tgatttttcc
    14461 atctttcgtg gtgacgtttt caggtgtgac taattgttca gacatatgct attccggcct
    14521 cgttattaca cgttatggcc cctggaggat tgaaaaaaga aacgccccgg tgagcggctg
    14581 ctcgtccggg ggcgctgcat tgtacaaatt ctggcgcacg gatgccacgt ttgcacgcgg
    14641 catttacgaa attattaacg ggtgcgcggg cacacttcgc cttcgccaaa gaaataagcg
    14701 atttcgcgag cggcagattc gacggaatca gaaccgtggg taccgttttc ggtcaggctg
    14761 tcagcgtaat cagcgcgcag agtaccagcc agtgcgtttg ccggattggt cgcgcccagc
    14821 agatcgcggt gacgctgaac ggcgttttca ccttccagca cggaaaccac gatcgggcca
    14881 gaggtcatga attcaaccag accatcaaag aacggttttc catcgtgttc agcataaaag
    14941 ccacgtgcct gttcaacggt caggtgcagc attttggtgc cgacaatttt gaaccctgca
    15001 gcttcaaagc gcgcaaagat attaccaatg acgttttttg ctaccgcgtt cggtttgatg
    15061 atggaaaaag tacgttcaat agccattttt acctctgtaa attgttctgt tgttgtctgt
    15121 accagcgtac gagatggcgc ggattataat gagcaacagg gccgttgact attgatgaag
    15181 gtaacatttt tttaaaataa aatgagtttt agcaacaatc aggggcaaaa cagactattg
    15241 catgacaaat ttcactgtcg cgatttgccc cacatcatcc atcaccagca attgataatc
    15301 acctttatcc gtcaaatgca gggtcacgtt gcgcccgcgt tcagttaacg gttcgccatt
    15361 cagaaaccac cagcgttcac ctgcccctcc acttgattgc aacggcaaag tcgcttccgc
    15421 tgcgcccggt aaacgtttaa taatcgcgcc atcgcgaacg cctgtcagct gcagagggag
    15481 cttggcatcg tgaccgtatg gcgggcaaat tgtcgaggcc ggtggtaagc gcacagcgcg
    15541 acgttctgat gcgggcagcc agggttccag cggtagcggc cagacattta tcatttcttg
    15601 tcgcgcttgc gggcaatcag cggcaacacg tttgccattt tcatccagcc agatggggaa
    15661 acgaatgcca ttaatgcctt cctgctccgg cagtaataga gttggcggct gactcccgtc
    15721 cagcagccag gttgccaggc ggcggcgaca gttaccgtca ccttccggca aagactgtcc
    15781 gcccggccag cagataacgc cacgagtgac tgagttcggg cgcgggtctt ccggcagatt
    15841 cgcactgcgc gacagtaaga tattattgac ctgattcaac aatggtacgg cactggcaaa
    15901 gccaaactga ccaacaacgg gcgtgccgtc cggtctgcca gtccagatcc caatgacata
    15961 gcgagcgtta accccaatcg cccaggcgtc acgatagcca tagctggtgc ccgttttcca
    16021 tgccagtggg gcgacgcgcg gcaaggcact atccggcaag ggttgcgctt catcagccat
    16081 aatccggcga atgatccacg ccgcccccga cgacattaaa ggccgttcaa gcagcggatc
    16141 gtcaggctgt aagcgcaatt tgcctgcctt gccgtggcga gcaaacgcgg tatacgctgc
    16201 cgccatatct tccagttttg caccagcgcc gccgagaatg agtgaaagat tcggcgcagc
    16261 accgttgggc aaatataacg gcaatccaac attgcgtaac tttgccgcaa accgtttcgg
    16321 tccataggct tccagcacct gcacagcagg taagttcagc gagcgcacca gcgcctcgct
    16381 catgctgatc gggccatgaa aaccgctatc aaagttacct ggtcgataat caccggtgcg
    16441 ccgggggacg tcttgcagca gtgatgccgg gtggatcaag ccttcatcca gcgccagacc
    16501 ataaacaaac ggtttgagca ccgatcctgg cgatcggatc gcattgacca tatcaacatg
    16561 accaaagcgt gaatcatcgt tgagatcaac cgatcccacc cagccgcgaa cacgcatatc
    16621 ggtatgatca accacgatca tcgccagtga gctgcgcggt ggcaaccgcc ctttccagtt
    16681 ttgcgccagt tcttccagac gtcgttgaag accggcatcc agcgtagtaa cgattttgtc
    16741 gcttttgctt ttaccgagca tcatgcgcga aaacagcggt gccagttgcg gcatttgtcg
    16801 gggtgccagc cagatgggtt cttcccttga ctcttttacc tgctcacggg accacacacc
    16861 ttgcacggcc atccgttcga gcactttatt acgcgcggct tcggcacgct ccggccaacg
    16921 atccgggcga agacggctgg gcgcttgcgg caaaaccgcc agcattgccg cctcggaata
    16981 gcttaaattc gcaggcgact ttccgagata agcccaactt gccgcaccga tcccctgcaa
    17041 cgtaccgcca aacggagcgc ggttaagata caaggtcaga atttcacgct tagacagatg
    17101 ccattccagt tgcaacgcgc gccagagctg gcgaattttg ccgccaaatg ttttggggtg
    17161 aggatcaagc agacgagcaa cctgcatagt gagcgtgctg ccaccggaaa taacccgtcc
    17221 cgaagtgaga tcttgccatg ctgcgcgcgc caccgagaat ggattcaccc ccggatgctt
    17281 ccagaaccag cgatcttcat aattgatcag cgcttcaagg taacgtggag aaacatcttc
    17341 gattgttacc ggataacgcc agatgccgtc agcatcggcg aagcgccata gcggcgtacc
    17401 atcctgcgcc acgaccactc gtgcgggatt gacttcatgc agcggtagag gccagagttt
    17461 atctgctccc cacgctgcaa gaaaaaggag aaatggcgca gctgccagcg ttatccagta
    17521 gccgcgtttg gttaacaagc gaggcattta cggtcggaca atcagcagat cttcagccgt
    17581 tccggtcgcc cgccattggg gaacatacat tgattccacc atcggttgcg gtacctgata
    17641 cgttcccggc gtcaccgccc gcgccagata caccagcgtt accggttggt attcatcaac
    17701 ggcaaccgcc gccacaaagc gatcgtcacg gaactcaatg tgcttaatac tcgcctgctg
    17761 catctggttc agtaagtttt gcacttcgcc accactttgc tccaggctgg cgctaccgtt
    17821 cgccagattc tgattttcca gttccagacc cgcaggcagc agatccacga ctaacgcatc
    17881 cggcacgctg ttactggctt ttacctgcaa ccagaccagc accagatcgc cgctacgtaa
    17941 cgagtccagc gatttgctct taccatcagt accaagaata tgacgctcga tttgcagcac
    18001 attgttcgcc ggtaaaggtg cggattgcgg ataaccgctg gcatccatac gcaaccataa
    18061 cggctgatcg ccactgtttc tcacctgcaa ggtggcaagt tgatcgctat tcagattgct
    18121 gtttagcgtt ttctcgcctg tcagcggctc agctgagaaa gaggtttgcg cctgccattt
    18181 acccggtaaa tcctgaatcg tacgggcagc caggaacaac gcgttacttt cctgcgtcga
    18241 tagccagcgt tcaccaaacg cctgctgcga aagtgtgttc agcaaagtgt actgctcatc
    18301 gggtagtagt ttattttctt ccagcaagga gagcattaac gcgttgtcgc gcagtggact
    18361 accgtaatca cccagccata tccgctcatc actatttctc ggcgttttca gcgccagcac
    18421 aatcgcttct tcaccacgcg ttgcatcacc catggttttc agcgcaacgc caagttgcag
    18481 cagcggtaaa ccagaagcgg catctgcacg atgctcccag atttcacgca gcgcacccag
    18541 tggtgctttt tgctgacggg ccagcaccag cgcagcgtaa gactgaacgg cgaatttact
    18601 ggctttgaga ttatccgcgt acgggatcga catcatgccc ggatcttgta aatagcgcag
    18661 cagacgctca ttaccccggt taatggcgtc tgtcggcacg ctgtaaccct gttcgcctgc
    18721 gcggaccagg aaatccatca cgtaagccgt cagccagtac tcttcgtcac cgtttttatc
    18781 ccacagcgca aagccgccgt tatcacgttg catttgcagc aaacgggaaa tgccgatatc
    18841 gaccgatgca cggcgtttct catcactgtc gcctttgatg cccaacgcct gcagttgggc
    18901 tgcgttggta taaagtgacg ggaacaggcc gctggcggtt tgctcaagac agccgtacgg
    18961 atacgctttt aactctttga tataacgtgc aatgttcagc ggtggtttgc cgctcaacaa
    19021 caattgccct tccagcgtaa caggcgagaa gttttgcaat ccatccgccg gaatcgccca
    19081 tgtctcacca ggctgtaacg ccgtaccgta attaaccgtt tgtgccggga acgccggacg
    19141 gacgccgatt ttccactgct tatgctgatc ggcaacggtt tcacccggta acgctaaccc
    19201 gctaatggtg gcctgaattt ctccatcgcc ataacccggc aatgctcgca ccgggataaa
    19261 cagcgtagta cgcacacctg gcgctaattc aacggctgcg ggtgaatcgc tgaccagttc
    19321 aagcaaacca ctggcggtca gggcaacgtt cagtttttgc ggtttatcgg taagattagt
    19381 gatatccagc gtcagacgcg aggtatcgcc actcgccata aagcgcggca tgttcagttc
    19441 agcaatcacc ggtgcggcaa ctatcacttt gctttcgttg ctaccgaaat catctgccgt
    19501 ccaggcttgc gccatgacgc gcaattcacc gttaaaatcg ccaatcggca gtgtaaccga
    19561 gccttcgccc tgttcgttga gcgttaccgg cagcgcctgc tgcgcgacaa tattgacgtg
    19621 attgaccggc ggtttaccac cacgtttcag ctcatcacca tcgccaccga aacgcagagc
    19681 tgccagacgc ccctgacctt caataacctg accgtaaata tcgtaaatgt ctgcgccata
    19741 gcgtttctga ccaaagaacg cctgccacgg atctggcgtg acgtagtcag taatattcag
    19801 cacaccacta tcaacggcag acaccagcac attcacctgt ttaggcttct cgccattttt
    19861 agtgctggct ttaattttca cggttaatgg ttgattggga cgcatttttg ctggtgtttc
    19921 cagcgccaga tcgaggcgac ggttttcatc gccaagcggc agatgcaaca caccaaccgc
    19981 gcgttttggc gtcgcggagc gagatttatc gccaggacgt accaccagcg tacttaaata
    20041 cagatcatga cgattccagg ttttatcgac cggaatcgtc agatccagcc cttgagcccg
    20101 aacatcaatc tcttgccacc acagcggccc ttcactggac tcgaccatcg cataaccttt
    20161 acccgccgtt ggcgcggcga tatgcaactt aatggtgtcg ccagggcgat aactggcttt
    20221 atccagtttc agcgtgacac ggtcgggtcg cactgcgccg ctaccgtcgc tgttgtcctg
    20281 ccagctatag ccagcccaga aacgaacact actgaccgct tcattcggcg ctttgacttc
    20341 cagacgataa gcgccccact ctaccggaaa actgaccttg ccggtttcgt ccgctttcag
    20401 atccagagtt tgttcatttt cgatcagatc tttttgatca aactgtgact gccagccttc
    20461 atcttctgac cagttccagt agtaatcgcg gcgttcgcga atcaggcgca cctgcaagcc
    20521 cgacacggct tttttcacgc cttgcgcatc gctataaacg atgtcaaaag cagcgttact
    20581 gccttcatca acaatgggct gtttcaccgt gctgtcagta cgataatcgt aaaccgattt
    20641 cgaggcgaac tgcggacgga tccccggcaa tgcatcggca ggccagatag cctgctcagc
    20701 gcggcgcgtc accgggcgac cgcccgattc cagcaaacta ccctggaaaa taacctgtaa
    20761 tggggaatgc gtttccttcc actggctttc tgtagaaact tcgccgcgcc ctttatcatc
    20821 cagcgtcaac tgaacttcat ccagcgtgcg ggaaagattt tcggcagcta tatcgccgaa
    20881 ttcaaaacca ggtaaggctg acacagcttc acgcagtggg cgcaggaaaa gttgcccttg
    20941 caaagtatta ccattagcag gtgcaccata caggtagtac cccaccacgg agaatttcac
    21001 ttcatctttc ggcgttagcg gggttttctc accggtcaga ttcagcgcca tgcgctctgg
    21061 cataaaatct tcgacgtgga aatcccacat ccgatactgg ttatcgcccg tgttagcgcg
    21121 aatatgccac ataccggttg ccgcattgct atcgagtggc caggtaaagt ggtagaggcc
    21181 attctccggc tgactaacga cgctcctgag tacctgccca tcgggtttaa tcacgtctaa
    21241 cttgatgggt tgattgggca acgctttacc gtctgcatca cgcagcaaac cattgaggat
    21301 taccgtttca cctgggcgat aaagatcgcg tgggccaaac atgaaaaact gtttgctata
    21361 gcctggcgcg ccagcaatgt taaattctgc taagtccagc gccggaagtt ttaaatcgag
    21421 tagcgttgtc tgaccgtctt tacgcgccaa cagtaatgcc gcgtttttat cattttccag
    21481 ctgcacatgc ccctgagcgt cactggttgc ctgagtcaga gtctgccctt tctcatttaa
    21541 taaagagact tcaattcctt gctgggccgc gccgttttcc agactttggg taaagatatc
    21601 cagacgattg tgataacggt gagctgaaac gccgatatca cttaacgtaa acagcgtcgc
    21661 gggattactg taatcgtaac gtccagcctg attcatcaca gccagataca cgcccgcctg
    21721 ctgaagcggt ttgatatcgc ccagcggcag caataatttt tcacgggtgt tacgcgcagg
    21781 attgagatca aaccgtccgg tgtagaccag atccgccatc tgcagcagtt tgtctgactg
    21841 ccagttcgcc agcgaattgc ggtattccca ttggctaatg aatgctggca gagattctgg
    21901 cttaacgcgg aagaagttaa catcgacatt attaacgttg agcgccatta ccggcagccc
    21961 ttcaacgact ttgccaggca gcagcgaacc acggctggca aaaccgacgc tgggttggat
    22021 gtcgcgggta gttatagttt tttcgtaatc tttactgaag gttgcgttgt tgagtgcttt
    22081 gacctccttg ccaatagtaa cgatcaaatc acgtttcggt tcgaggtggc gtaaacgtaa
    22141 ctctttaaga ttatctgaca gctcccaggc accatccact ttgccgcttt ttttatcgac
    22201 gacatgaata acgcgtgaga aatcctgatc cgggtcgaga gggatggaga acgtcagcac
    22261 cagcgtggct gcaccatcaa gttggacttc agagagatcc agcaatgtca gcaccttacc
    22321 ggcactctgt tgggccagtt tttgtctctc cggcacggag agttttgctg aactcgcgtt
    22381 ttctgaagag gccgctttag taacttcaga aggcgcatct tttttcaccg ctgttggcgc
    22441 gttatcgttg ttgtcgcacc ctgccagcgc cagcattagc atgcaggcgg ctacgcgtaa
    22501 ctttttcatt tttcatccct gccacaatgg cccgttagca acgtcgaata attattatgc
    22561 gtgagaattt acgttatgta attcagtatc acgcccatac aatccgaatt ttagtgagaa
    22621 ttgtggctca gacagccaaa tcagcctctt tcgcttgtcg cttttcacta aacatccgac
    22681 aatttagcct aaccccggca aaaatggaga tgcctatgtc cacgacatgg tttgtaggag
    22741 ccgactggct cgccgaacat attgatgacc cggaaattca gattattgat gcccgcatgg
    22801 cgtcgcctgg acaggaagat cgtaacgttg ctcaggagta tctgaatgga catattcccg
    22861 gcgcagtgtt ttttgatatc gaagcgcttt ctgatcacac ttccccgctt ccgcacatgc
    22921 tgccgcgccc ggaaacgttc gccgtggcga tgcgtgaatt aggcgttaac caggataagc
    22981 acctgattgt ctatgacgaa ggtaatcttt tctcagcccc acgagcatgg tggatgctgc
    23041 gcacctttgg tgtagagaaa gtgtcgattc tggggggtgg acttgcaggc tggcagcgcg
    23101 atgatctgct gttagaagaa ggtgcagtag agctgccgga aggagagttt aacgccgcgt
    23161 ttaatcctga agccgtggtg aaagtaaccg atgtattatt ggcaagccat gaaaatacgg
    23221 cgcaaattat tgatgcccgc ccggctgcac gttttaacgc agaagttgat gaacctcgcc
    23281 caggtttacg tcgcggacat attcccggag cactgaatgt tccgtggacg gaactggtgc
    23341 gcgaaggcga actaaaaacg accgatgaac tggatgcgat attttttggt cgcggcgtca
    23401 gctacgacaa accaattatc gtcagctgcg gctctggtgt aacggcagcc gtggttttgt
    23461 tagcactcgc gacgctggat gtgccaaacg tgaaactgta cgacggcgca tggagtgaat
    23521 ggggcgcgcg ggcagattta ccggttgagc cagtgaaata agtattttac aggcaataaa
    23581 aaaccgccga atttggcggt tttttattgc tggtctggtt cgcggccttt ccagcaggtt
    23641 gtattaccgt agtaatgcaa gcgcgtctca gcggagacaa tactcgccag taactctctt
    23701 tttgtcaagc aaaagagagt tattattgtt ctgttagtgt attatccact gcggcccttt
    23761 ccgccgtctc gcaagcgggc gctggcttaa ggaaaggatg ttccgcgacc gttagagcaa
    23821 gcgcgtctca gcgcttgcta tcgcggcaat atcgccagtg gtgctgtcgt gatgcggtct
    23881 tcgcatggac cgcacaatga agatacggtg cttctgtatc gtacttattg tttctggtgc
    23941 gctgttttcc caagtaaata ataaccggag tctctccggc gacaatttac tggtggttaa
    24001 caaccttcag agcagcaagt aagcccgaat gccgcccttt ggggcggcat attttagatt
    24061 atccgattct gtttaaagtc acgcaaaaaa ccaccccagc gacgttcata gaatggcaca
    24121 atatgttcgg taataaagtg gctaattcct ttttcccctt ttttcacctg acaaatatcg
    24181 attggttcat cgccaggtaa tgtatcggtc gctacacttc ccgtcgcctg aataatttct
    24241 tcgatatcac catcggcttc aatgccaata agtaaattag gctgtgcctc ttcgttctct
    24301 ttaattgaac aaataaaagc acgcttcacc gacttaatgg ttttaaataa ggtggtgagt
    24361 gaatcaatca tttgtgctgg cggctctgcg acttccgata atatcagcga ttcaccgcct
    24421 tccaggattt cctggctgct cagcggattt ccctcttcac caatcaacaa actgatttca
    24481 cgcggcataa attctttacc ggtcggcagt ttggcattaa ggaagagcgt ttcgccaagg
    24541 gtcatctcaa acaatgtgcg aacgggcatt acgacaaatg cctgttcgtc ttcaaccgcc
    24601 tgttgaagcg cttctaacga ggtgaaaaaa ggaatgacgc tggtgccgtc ttctttttcc
    24661 cagtgctgta aatcaagcgc actatcttca accacagcct cgccctgcgc cgccgtacca
    24721 ggcacccaga cggtggattc cagtagagta cggaaaaagg ccgggcggtg cgccggttcg
    24781 gttgctgctt tttccagcag gtcttcaagt tcgttttttg tttcggacat aagaaccaca
    24841 attcattcaa cgttcggggc gcaaatgcct gatgcgctac gcttatcagg cctacaaggg
    24901 attcgcaatt tgttgaattt gcagaatttg taggccggat aaggcgttta cgccgcatcc
    24961 ggcatattag tttacgccgt taacagatta gcaatcgtgc gcacaccaag tcccgtagcg
    25021 cccgcagacc actgttcaac cggcgcttta cggtaagtcg ccgagcagtc gatatgcagc
    25081 cagccttgct gatagttctc aacaaagtgc gacaggaagc ccgccgccgt gctcgcgcct
    25141 gccggatacg ccgcgcttcc ggtattgttc agttcggcaa agttagacgg cagctggctg
    25201 cggtggaatt ccgccagcgg cagacgccag aacggttcgt tctcttgtga ggcactcgcc
    25261 agcaaacgac cggcaagcgc atcgtcaaaa ctgaacagcg cgtgataatc attacccagc
    25321 gcagttttcg ccgccccggt gagggtcgcc gcatcaatga tcagttccgg tttctgcgca
    25381 ctggcatcaa tcagaccatc ggccagcacc agacgccctt cggcatcagt gttcatcact
    25441 tcaacttttt taccgttgcg ataggtgatg atatcgccca gcttgaacgc attgccgcta
    25501 atcaggttat ccgcacagca gaggaacagc ttcacgcgct tgttcagtcc gcgcgtaatg
    25561 gcaaatgcca gcgccccggt aaccgttgcc gcgccgccca tgtccgactt catcgagtcc
    25621 ataaacgcag tctgttttat gctgtagccg ccggagtcaa aagtgatacc tttacctacc
    25681 aggcacgcgt acaccggtgc ttctttatcg ccagttgggt tgtaatccag cgccagcaat
    25741 accggagaac gttctgaacc gcgcccgaca gtgtgcagcc ccatataacc ttgctcacgc
    25801 agatcttcgc ctttggtgat ccgataagtc acacgatcgc ccgcgacgtt gctgatcaga
    25861 tcaacagcac gctgtgccag ttgcgatggt cccaattctt ctgccggtgc gttgatggtg
    25921 tcacgcaccc agtcgatgat catcaggcgg ttatccagtt cctggcgctg ggcatcgtcc
    25981 agatccggcc actccacttt acgcgtgcct ttcggggctt tgtaaccttg ccagaatgcc
    26041 cagcagcgat ccgcatccca gccttcaccg cttaactgaa catgcttgat gcccagaccg
    26101 tcaatcttgc gcgccgcacg ctggatcagc cccagatcgt ctgccccgtt caaatgcagg
    26161 gtaatgccgt cattattaat gctgtaagtt gctttttctc cccagcgcgc gtcggcaggt
    26221 tgggtagaga gggtaatctt catcgcttct gtcattttag ttatccttct ttgtaaaagg
    26281 gccgccagtt ggcagcccca ggatttttcc gctaaatgat tcgcgttgca ggaaggcggc
    26341 aagtgagtga agccccagga gcatagataa ctatgtgact ggggtgaacg agcgcagcca
    26401 acgcatctgc ggcgtgaagc atgacgcgga aattattcgg cctcgtccag ccagactaac
    26461 aaaatcgctt cgaggatttt ctcgttggat gcctgcgggt cgtcgtcgaa atcttccaaa
    26521 tcgcaaatcc actgatgcat atcggtgaat cgaaccgttt tcggatcaag atcgggatac
    26581 gcatcgtaca gtgcttcgcc aatttcgcgg ctatcggtcc acttaagtcc catactaacc
    26641 tctgttaatg ctcacgcgca tggttgatag tgtaacgcgg gatttcgacc actaaatctt
    26701 cgtcggtgac gcgcgcctgg cagcttaaac ggctttccgg ctccagtccc caggctttgt
    26761 ccagcatgtc gtcttcctgc tctgagcttt ccggcagcga gtcaaaacct tcacgaacga
    26821 tgcagtggca ggtggtgcaa gcacaggatt tttcacaggc gtgttcaatc tcgataccgt
    26881 tacgcagagc tgcgtcgaga atggtttcac cgctattagc ttccagaaca gcgccatcag
    26941 ggcagagatc ctgatgaggc aaaataacaa tctttggcat attaaacctc gtccacggaa
    27001 tggcctttca gcgcacgacg aaccgactgg tccatgcggc gagcggcgaa atcctgggtt
    27061 tgtttgtcta cgtttttaat cgcttgttcg atggcgtcaa cgtcatcgcc ctgcgccact
    27121 tcactcaggt gagcggcagc atcgtcaatg acctgacgtt ctgcggcgct taacagcgcg
    27181 gcatcagcag ccagcgcgcc gtgcagactt tccagcacac gcgccgcttc tactttttgt
    27241 tctgccagca ttcgggcttt tacgtcctgc tcggcatagc tcattgagtc tttgatcatc
    27301 gaagcgattt cgctatcggt cagaccgtaa gacggtttga cctgaataga cgcctcaacg
    27361 ccggtggatt tctccatcgc cgtcacgctc aaaagaccgt cggcatcgac ctggaacgtc
    27421 acgcgaatat gcgcaccacc agccggtagc gccggaatac cacgcagcgc aaaacgcgcc
    27481 agtgagcggc agtcctgcac cagttcgcgc tcaccctgca ttacatggat agacatcgcc
    27541 gtctgaccat ctttaaaggt ggtgaaatcc tgagcgcggg ccaccggaat cgtggtatta
    27601 cgcggaatca ctttctccac caggccgccc atcgtttcga ggcccagcga cagtgggatc
    27661 acatcaagca gcagcatttc gctgtccggc ttgttaccca ccagaatatc cgcctgaatc
    27721 gccgcgccaa tagcgacgac tttatccggg tcgatggaag tcagcggtgg acgaccgaaa
    27781 aattcgccta cccgttcacg caccagcggc acgcgagtag aaccgcccac catcaccact
    27841 tccagcactt catcagcttc tacacccgcg tctttcagcg cgcgacgaca agccagtaag
    27901 gttcgtttta ccagtggcgc gatcagttga ttgaattgtt cacggctgat ttcgccctgc
    27961 cagcccgcaa cgttaacggt cacggagtcc gcatcgctca gcgcgatttt ggctgcaatg
    28021 gcggcatcca gcagttcacg ctgaacgcgg ttatcgctac gatcaggaat gcccgcctgc
    28081 tcgcgaatgt aatccgccag cagatggtcg aaatcatcgc cgccgagcgc ggaatcaccg
    28141 ccggttgcca gcacttcaaa cacgccgcga cttaagcgca gaatggaaat atcaaacgtc
    28201 ccgccaccga ggtcataaac agcgatcacg ccttcctgac cggaatccag cccgtaggcg
    28261 atagccgcag cggtcggttc gttaagtaag cgcagaacat gcaaccccgc cagacgcgcc
    28321 gcgtctttgg tgccctgacg ctgggcatcg tcaaagtacg ccggaacggt gataactaca
    28381 ccatccagct cgcctgccag ggcttcagtt gcccgcgccg ccagtgcttt gaggatgtcc
    28441 gcagaaacgc gcaccgggtt cagcagcccc gccgccgttt caatcatcgg caggccgttt
    28501 tcgctggcct ggaattgata aggcagatgc ggatagcgtt gctggatatc agccagcgag
    28561 cgccccatca ggcgtttaac agaactaatg gtgttggcgg tatcaagcgc tgcgttagta
    28621 cgcgcgtcat aacccaccga atgcccttgc tgttgatagt gaacaacaga tggcagcagg
    28681 tgacggcctt catggtcggc taacgtttcg gcctgaccgc tgcgcactgt cgccaccagc
    28741 gagttggttg tgcccaggtc aataccagcc gccagacgac gctgatgcgg cgcagcactc
    28801 aaaccaggtt cactaatttg taataaggcc atgtttagct tccagaaatt aaaaatcgag
    28861 cagtttttct tcgagttgtt cggcactgct ccgcagttta tcgagaaaac gcagcttacg
    28921 cacggtatcc gccgccgcgt cccacgcctc gttgtctaac tgttcaacca tcaactgatg
    28981 gcgggtatca aacatctttt tcacacgttt gataaagctt tccagccgcg cttcatcttt
    29041 cgcctgttcg atctcgtcca gctcttcgcg cagctccaac tgttccatca ggaacgcggt
    29101 gtcgcgcaca gtatgctgct cgctggcgag atcaaaaccg tgtaaagaaa gcaaatattc
    29161 cgcgcgcatt aacggatgac gcagcgtttg ccaggcctgg ttaatggttg cagattgctg
    29221 tacggcggcg agttgttccg cctggcttcc gctggcgaat ttatcaggat gatactgacg
    29281 ttgtagatcc tgaaaacgca ggctcagcgc ctgggtatcg agttgataac gggcaggcaa
    29341 gccaaagagg gtgaagtaat ccataacatt ctcagggctg cgatccgccg cgataacggc
    29401 gaatcaggta aaacaaaccc cacgcgcagg cgaccacgat ggggttatcg gtatgcgcat
    29461 caaacgtgga agctttcgcc gcaaccacac tcatctttga cgttcgggtt ggtgaattta
    29521 aacccttcgt tcaggccttc ttttacgaag tccagctgcg taccgtccag aaattgcagg
    29581 cttttgccat cgaccacgac tttcacgcct ttgtcttcaa acacgatgtc ttccggcgtc
    29641 ggttcgtcaa caaattccag tacataagcc atacctgaac atccggaggt tctcacgccc
    29701 agacgcaggc caaacccttt gccgcggtta gccagaaagg tatttactcg cgctgctgca
    29761 ctgtcgctca gtgtaatcga cataaccaaa cctcaactct tattttgctt cacgtttgct
    29821 tttatagtcc gcaatggcgg ctttgatcgc gtcttctgcc agaatagaac agtgaatttt
    29881 caccggcggc agttcaagtt cttcagcaat atcggtattt ttgatcgcct gcgcttcgtc
    29941 gagagacttc cctttcaccc attcggtgac cagggagctg gaagcgatag cagaaccgca
    30001 gccgtaagtt ttaaaacgcg cgtcttcaat gataccttca tcgttgactt taatctgcaa
    30061 cttcatcacg tcgccacagg ccggtgcccc caccatgccg ctgccgacgt tctcgtcgtt
    30121 gttgtcaaag gaacccacgt tacgcggatt ctcgtaatgg tcgataactt tttcgctgta
    30181 agccattata aattctcctg attccgatac cgattaatga tgagcccatt cgatgctgtt
    30241 cagatccacg ccctgcttgt acatttccca cagcggagaa aggtcacgca gacgaccgat
    30301 ggatttacga actaactcga tggtgtagtc gatctcttct tcagtagtaa aacgacctaa
    30361 agagaaacgg atagagctat gtgccagctc gtcgttcagc cccagcgcgc gcagcacgta
    30421 ggacggttcg aggcttgctg acgtacaggc ggaacctgaa gaaactgcga ggtctttcag
    30481 cgccataatc agcgactcac cttcaacgta gttgaagctg acgttgagaa tgttcggcgc
    30541 accgtgttcc aggtcaccgt tcaggtaaac ttcttcgata tctttgatgc cgttccacag
    30601 acggttacgc aggccgcgca gacgttccat ctcggtcgcc atctcttctt ttgcgatgcg
    30661 ataggcctcg cccattccga cgatctggtg aacaggcaga gtgccggaac gcataccgcg
    30721 ctcgtgaccg ccgccgtgca tttgcgcttc gatgcgtacg cgcggtttac gacgtacata
    30781 cagcgcaccg atacctttcg ggccatagat tttgtgaccg gagaaagaca tcaggtcaac
    30841 tttcaactgg ctcaggtcga taggcagttt gcccacgctc tgggttgcat caacgtgata
    30901 gataatgcca cgagcacggc acatttcgcc gatagccgcg atatcctgca ccacgccgat
    30961 ttcgttattt acgtgcatga tggaaacaag gatggtgtcg tcacgcatcg ctgcttcaag
    31021 ttctttcagg tcgataatgc cgttacgctg cggtgccagg taggtgactt caaaaccttc
    31081 gcgctccagc tgacggcagg tatccagtac cgctttgtgt tcggttttgc tggtgatgat
    31141 gtgcttgcct tttttctgat aaaagttggc tgcacctttg atcgccaggt tgtcagattc
    31201 ggttgcacca gaggtaaaga cgatttcacg cggatcagcg ccgaccagat cggcaatctg
    31261 attacgggcg atatctaccg cttcttcagc ctgccagccg aaacggtgag aacgagaggc
    31321 cgggttacca aaggttccgt ccatcgtcat aaactgcatc attttctcgg caacacgcgg
    31381 gtccaccggc gtggttgcgg agtagtcgag ataaatcggt aatttcattg ctctataaac
    31441 tccgtacatc actcaatgca aggaaccagg ctaccggctg gatgtacgac cgtgtttacg
    31501 gagcgcgata cgccccggcc tgattctgaa ttctttttat taagcgcgta acttaacgtc
    31561 gatcgcgtct tgtatgcggg tgcgtggcgc gtcgtgagta tgctgacgac cagacacatc
    31621 cagcacttcc tggttattaa ccagttcgcc taaagtaatg ttgttgagaa aaccggtgag
    31681 acggtcgctc aaatcacgcc acagcgcgtg ggtcaggcat ttatcgccgc cctggcagcc
    31741 gcctttaccc tgacaacggg tggcatctac agattcgtca acggcgctaa tcacttcgcc
    31801 aacggcgatg ctgctggcat ctttgcctaa cagataacca ccgcctggtc cacgtacgct
    31861 ggaaaccaga ccatttttac gcagacggga aaacagttgt tccagataag aaagggaaat
    31921 tccctgacgt tcggaaatat cagccaacgg taccgggccc gcttcagagt tgagcgcaac
    31981 gtcaagcatt gcggtcacgg catagcgccc tttagatgtc agtctcatgt cttacttcac
    32041 ctcaaactcg cccctgcccg gggtttttta ttgtaaaagt gggggtattg catagcaggg
    32101 tcaagtctga cattcccgag taaattggtc aactatttac ttgactgatt tagtcgggta
    32161 tttaaccttc agtgccattt ttttatcgtg gcgtttgtac ctgttgtcct tccagaccat
    32221 gggcgggttg agggcaatta ttcgcattag cgctggcctc ttcccctcac cctaaccctc
    32281 tccccgaagg ggcgagggga ccgtccgtgc atttatctgg cttagccctc aggccttatt
    32341 acctttattc tgctgctcaa tagaagccag aatcccgcgc aggatattca attcctggct
    32401 ttccgggcgc gcacgggtaa acagacggcg caatttattc atcacctgcc ccggatggtt
    32461 ttcacggata aaaccggttg ccagcagcgt ttgttccaga tgaccataaa aacgctccag
    32521 atcatcgacc agcggatacg gcgtctcttc atgctcgacc tgctcgccgt tttcctgagt
    32581 tgccagccag gccatgcgca cttcataggc aataacctga accgccatcg ccaggttcag
    32641 cgagctgtat tccgggttag ccgcaatcgc gacatgataa tggcatttct gcaactcttc
    32701 attggtcaag ccgacgcgct cacgaccaaa caccagcgcc actggggtat ttgccgcttc
    32761 agcgacgctt ttcagaccgc attcgcgcgg gtcgagcatc ggccacggca gcgtgcggga
    32821 gcgtgcgctg gtgcccacca ccaggctaca accggctaac gcttcgtcga gcgtatcgac
    32881 gatgtgagca ttaccaatca catcgctggc ccctgctgcc agggcaatcg cctgggagtc
    32941 gggtttcacc agtggattaa ccagccacag attggttaat cccattgttt tcatggcacg
    33001 ggcaacagaa cccatattgc cggtgtgtga cgtctccacc agcacaattc gaatattttg
    33061 cagcattgtc tttcttcatc taaagattat tcacgcatct tatcataaaa cgaagacaga
    33121 tgccgatctc gctgctatac tctgcgccgt tttcccgttc tttaacatcc agtgagagag
    33181 accgatgcat ccgatgctga acatcgccgt gcgcgcagcg cgcaaggcgg gtaatttaat
    33241 tgccaaaaac tatgaaaccc cggacgctgt agaagcgagc cagaaaggca gtaacgattt
    33301 cgtgaccaac gtagataaag ctgccgaagc ggtgattatc gacacgattc gtaaatctta
    33361 cccacagcac accatcatca ccgaagaaag cggtgaactt gaaggtactg atcaggatgt
    33421 tcaatgggtt atcgatccac tggatggtac taccaacttt atcaaacgtc tgccgcactt
    33481 cgcggtatct atcgccgttc gtatcaaagg ccgcaccgaa gttgctgtgg tatacgatcc
    33541 tatgcgtaac gaactgttca ccgccactcg cggtcagggc gcacagctga acggctaccg
    33601 tctgcgcggt agcaccgctc gcgatctcga cggtaccatt ctggcgaccg gcttcccgtt
    33661 caaagcaaaa cagtacgcca ctacctacat caacatcgtc ggcaaactgt tcaacgaatg
    33721 tgcagacttc cgtcgtaccg gttctgcggc gctggatctg gcttacgtcg ctgcgggtcg
    33781 tgttgacggt ttctttgaaa tcggtctgcg tccgtgggat ttcgcggcag gcgagctgct
    33841 ggttcgtgaa gcgggcggca tcgtcagcga cttcaccggt ggtcataact atatgctgac
    33901 cggtaacatc gttgctggta acccacgtgt tgtgaaagcc atgctggcga acatgcgtga
    33961 cgagttaagc gacgctctga agcgttaatg actcaggcgg gtgatgtcac tcacccgccc
    34021 tcgcctttca ggcgctattc tgaaatactt cctcaccgct ttactttctt tcgtcactct
    34081 cccaccatct ttccccgatt aatggatgat aaagaagtcg caaggaaagg ttatggcact
    34141 gccagtgaac aaacgcgttc ccaaaattct gtttattctc tttgttgttg ccttctgcgt
    34201 ttatttagtg ccgcgcgttg ccatcaactt cttctattat cctgacgata aaatttacgg
    34261 tcccgacccc tggtcggcgg aatccgtcga atttacggct aaggacggta ctcgtctgca
    34321 aggctggttt attccttctt cgacgggccc tgctgacaac gccatcgcaa ccatcattca
    34381 tgctcacggc aatgccggaa atatgtccgc ccactggccg ctggtcagtt ggttacctga
    34441 gcgtaatttc aacgttttta tgtttgatta tcgcgggttt ggtaaatcaa aaggcacgcc
    34501 gtcccaagcc ggattgctgg acgatacgca aagtgccatc aatgtggtgc gccatcgcag
    34561 tgatgtaaac ccacaacgtc tggtgctgtt cgggcagagt attggcgggg cgaatattct
    34621 ggatgttatt ggtcggggtg atcgtgaagg catacgtgcg gtgatcctcg actccacatt
    34681 tgcctcttat gcaaccatcg ccaaccagat gatccccggc agtggctact tacttgatga
    34741 gagttacagc ggcgaaaatt atatcgccag cgtcagcccg atcccgcttt tactcattca
    34801 cggtaaagct gatcacgtta tcccatggca gcacagcgaa aagttgtata gcctggcaaa
    34861 agagccaaaa cggttgatcc taatcccgga tggcgaacac attgatgctt tttccgatcg
    34921 tcacggcgat gtttatcgcg aacagatggt ggactttatc cttagcgcgt tgaatccaca
    34981 gaactaaccc atgatcgcta gcacgataat cattcacaaa accaccttaa gacatgctaa
    35041 tccactggtc agaacagttt aagatgagaa aaattctgtg acgcttgcca acatttctga
    35101 tgattagcat tcccttcgcc atttccctga gcaaacttta gctattctta tcaattatgc
    35161 ttatgggaga tctacagatg atgcctacgc ttgctccacc atctgtcctt tcggctcccc
    35221 agcgccgctg ccagatcttg ctgacgctct ttcagccggg gttgaccgcc accacggcaa
    35281 ctttcagcga gcttaatggt gtggatgatg atattgccag tcttgatatc agcgaaacag
    35341 gacaggagat cctgcgctat catcaactca cactgacggc tggttatgac ggtagctacc
    35401 gggttgaagg tacagtgctt aaccaacgtt tgtgtttatt tcactggcta cgacgtggtt
    35461 tccgtctgtg tccatcattt attaccagcc atttcacccc cgccctgaag agtgaactga
    35521 agcggcgcgg aattgcgcgt aacttttacg acgataccaa tctacaagcg ttagtgaatc
    35581 tctgctcccg acgactgcaa aaacgctttg aaacgcgcga tattcatttc ctgtgtctgt
    35641 atctgcaata ttgtttgctg caacaccacg ctggaattac gcctcagttt aatccgctcc
    35701 aacgtcgctg ggcggaatcc tgccttgaat ttcaggtagc gcaggaaatt ggacgccact
    35761 ggcagcgtcg ggcgctccag cctgtaccgc ctgatgagcc actgtttatg gcactacttt
    35821 tttccatgtt gcgggttccc gatccactgc gggatgcgca tcagcgagac agacaattgc
    35881 gtcagtctat caaacgtctg gtaaaccatt ttcgtgagct gggaaatgtt cgtttttatg
    35941 atgaacaggg gttatgtgat cagctttata cccacctcgc ccaggcgtta aatcgcagtt
    36001 tgtttgccat cggtattgat aataccctgc cggaagagtt cgccagactg tacccacgcc
    36061 tggtgcgcac cacccgcgcg gcgctggccg gatttgaaag tgaatacggc gtccatcttt
    36121 ctgatgagga aagtggtctg gtcgcggtga ttttcggtgc ctggctaatg caggaaaacg
    36181 atctgcatga aaaacagatt attctactga ccgggaatga tagcgagcga gaagcgcaga
    36241 ttgagcagca gttacgcgaa ctaacgttac tgccgctcaa cattaagcat atgtcggtaa
    36301 aggcattttt gcagacaggc gctccgcgtg gcgcggcact gattattgcg ccttatacca
    36361 tgccattacc gctcttttca ccaccgctga tctatacgga cctgacgctg acaacacatc
    36421 aacaggagca gatccgcaaa atgctcgaat cagcatgagg gaacaacttt cgggcgcaaa
    36481 aacattgccg gcagcgccac cagcgccatt acccagaaca cgccgtggcc cagatattga
    36541 tacaggaaac cggcgaaaac ggtcatgata gcgatactgc cgcccatcgc gacggcagag
    36601 taaaccgcct gtaaacggat gacttcgcta ccctggcgag cagcaatata acgcatggcg
    36661 gccaggtggc agaccgtgaa ggtgccgcaa tgcagaattt gcaccactat caaccacggc
    36721 aacgccgtag ttgctcccat aatgccccag cgcactacgc cgcaaatcgc cgagatcaac
    36781 agcatatcgc gtgcactaca acggcggaaa agtttattac tcagcgcaaa gataatgact
    36841 tccgccacca cgcccagcga ccacaaatac cccaccgccg aggccgagta gccagctgcc
    36901 tgccagtaaa tggcgctaaa accgtaatag gccgcatgtg ccccctgcaa taaacaaacg
    36961 caggccagaa agcgccagtt ctggcgaacc agcgccaacc acgcagacca accggtgctc
    37021 tcctgctggc gacttgcccc ttgtggctga atcgtcggac ggatgagaaa gccgagcagc
    37081 atggatgcca cgcccaacgt caacagcgcg aggatcaccc gataatcaaa catagtgacc
    37141 agtttgcccg tcagcgccga gccaatgaca aacgccaccg agccccacag tcgcactttg
    37201 ccgtaatcaa gcgggaactg cttttgccac gtattcgcca gtgcatcggt caacggtacc
    37261 agtggtgaga aaaagaggtt aaagccaatc atcaccagca tcagccacgc tacgtgcgcc
    37321 cccgcccaga aggcgacagc aaagagaagt gtcagcagtg ccagcacgcg caaggcggaa
    37381 atcaggcggg aaggatcgct gacgcggggc gcgatgagca aactcccgag gaaacgggca
    37441 accagacctg cccccaataa caggccgatg gtttctggcg ttaaaccaat ccctttaagc
    37501 cagacgctcc agaaaggtag aaaaatgccg taactaaaaa agtatgtgaa atagccgagc
    37561 gccaaccagc gcgtggattg caaaaccatg agtccctccc gtcaggaggc gttagtctgg
    37621 ggttaatcga atgttggtgc aagttgaaaa ataagaatat tcgagcctgc ttcccgatgt
    37681 ctgtaacgcc agttcacaag cactaaaaaa ctgcgatccg cgacgcatgt tgcaaaaata
    37741 atgcgtaatt atgccgttac gcttgccaaa cgttcctgca caatggcgat gaaatcgcgt
    37801 aatgccggct tcatctcccc tttcttccac gccatcagta aagcaatgga aggcacattt
    37861 ccggcaatgg gacgaaaaac aacctgcccg gtattaaaat tattcatata accgggtatc
    37921 aaagtgacgc ccagccccat gcccaccaga ttcatggtca ccagaatatt cgttgccacc
    37981 tggacgatat ttggctggct gttttcttgc gcaaaccacg ctttaacgat cggcgcaagc
    38041 gaaccggaat acgccggatc ggtactgacg aaattcacgc catccagttg cgcggcggtg
    38101 atctcttttt catgtgctaa agggtgatca accggtaaca caaccactaa tggttcgtca
    38161 aaaagctcca gataatcaat ctccgggcta taaacaggat ggcgcattaa gccgacgtcg
    38221 agttcaccac ggcgaatttt ttcctcctgt tgcgtggtga ttaaactcac cagctcaatc
    38281 aaggtgtctg gctgtctgag acgaaacatc ggtaatactt ttggcagtaa attcacttcc
    38341 gccgatggca caaagccaat ggttaattgt ctgtcttcct gaacaatttt ccgcgcccgt
    38401 aatttggcat tttccgcttg ttcgaggatt gccagcgcgt cctggagaaa acattctccc
    38461 gccgccgtca acgcgacttt gcgcttatcc ctcaccagta acggaacacc gacacagttt
    38521 tcaagatcgc ggatctggct gcttaacgaa ggctgtgagg tatgcagttt ttccgccgca
    38581 cgggtaaagt tcagtgcctg cgccactgcg acgaaatagc gtaaatgccg tagttccatc
    38641 accttcccct tgttatcgaa aaaacgtctc agctggtaga aattaactat ttcacatatt
    38701 agcaaccaac cagcaacatc cttatggcac aaaaatagaa ggtcaataca tcttatcttt
    38761 caggattaaa aaatatgacc acaccctcag atttgaacat ttaccaactg attgacaccc
    38821 aaaatggtcg ggtcactccg cgtatttata ccgacccaga tatttaccaa ctggagcttg
    38881 agcgtatttt cggtcgttgc tggctatttc tcgcccacga aagccagatc ccaaaacccg
    38941 gtgatttctt taacacctac atgggagaag atgcggttgt cgtagtgcgt cagaaagacg
    39001 gcagcatcaa ggcgtttctc aaccaatgcc gccaccgggc catgcgtgtg agttatgcag
    39061 attgcggcaa cactcgcgcc tttacctgcc cgtatcacgg ctggtcttat ggcattaacg
    39121 gcgagttgat cgatgtaccg ctggaacctc gcgcctaccc acaagggttg tgtaaatccc
    39181 actggggact aaacgaagtt ccttgtgtgg agagttataa agggctgatt tttggcaact
    39241 gggataccag cgcaccgggc ctgcgtgatt acctgggtga cattgcctgg tatctggatg
    39301 gcatgctgga tcgtcgcgaa ggcggcaccg aaattgtcgg cggcgtacaa aagtgggtga
    39361 tcaactgtaa ctggaaattc ccggcagagc agttcgccag tgaccagtat catgctctgt
    39421 tcagccatgc ttctgccgtt caggtattag gggcaaaaga tgatggcagc gataagcgcc
    39481 tcggtgatgg acaaaccgcc cgcccggtgt gggaaaccgc caaagatgcg ctgcaatttg
    39541 gtcaggacgg tcacggtagc ggtttcttct ttactgaaaa accggatgct aatgtctggg
    39601 tcgatggcgc agtttcaagc tattaccgcg aaacctatgc cgaagcagaa caacgtttag
    39661 gtgaagttcg cgccctgcgc ctggcgggtc ataacaatat attccccacg ctttcatggc
    39721 taaacggcac tgccacactc cgcgtctggc atccgcgcgg ccctgatcaa gttgaagtgt
    39781 gggcgttctg tattactgac aaagccgcct ccgatgaagt taaagccgct tttgaaaaca
    39841 gcgccactcg tgcttttggt cctgctggtt ttctcgagca ggatgactcg gagaactggt
    39901 gtgaaatcca gaaattgctt aaaggccacc gcgcccgcaa cagcaaactg tgtctggaaa
    39961 tggggcttgg tcaggaaaag cgccgcgacg acggcattcc tggcattact aactatatct
    40021 tttcagaaac tgccgctcgt ggaatgtacc aacgctgggc cgatcttctg agtagcgaaa
    40081 gctggcagga agtgctcgat aaaaccgccg cttaccagca ggaggtgatg aaatgagtgc
    40141 gcaagtttca ctagagttac atcaccgcat tagccagttt ctctttcacg aagccagctt
    40201 actggacgac tggaaatttc gtgactggct ggcgcagctc gacgaagaga ttcgttacac
    40261 catgcgcacc acagttaacg cgcaaacacg cgaccgccgc aaaggcgtcc agccaccgac
    40321 aacctggatt tttaatgaca ccaaagacca gctggagcgg cgaatcgccc gtctggaaac
    40381 gggcatggca tgggcagaag agccgccgtc acgcacccgc cacttaatca gcaactgcca
    40441 gataagcgaa accgacatcc caaacgtatt tgctgttcgg gtgaattatc tgctttatcg
    40501 ggcacaaaaa gagcgcgatg aaacgttcta tgtcgggact cgttttgaca aagttcgccg
    40561 tctggaagat gacaactggc gcttgctgga acgggatatc gtcctggatc aagcggtaat
    40621 cacttcccat aacctgagtg tactgttctg atgaatcgaa tttatgcgtg tcccgttgcg
    40681 gatgtgccgg agggtgaggc tctccggatc gatacctcgc ccgtcatcgc cctgttcaac
    40741 gttggcggcg agttttatgc cattaacgat cgttgcagcc atggtaatgc gtcaatgtca
    40801 gaagggtatc tggaagatga cgccacggtg gagtgcccgc tacacgccgc cagtttttgc
    40861 ctgaaaacgg ggaaagcgtt atgcctgccc gccaccgatc cgctcaccac ttatccagta
    40921 cacgttgaag gtggtgacat tttcatcgac ttaccggagg cgcagccatg agcgatctgc
    40981 ataacgagtc catttttatt accggcggcg gatcgggatt agggctggcg ctggtcgagc
    41041 gatttatcga agaaggcgcg caggttgcca cgctggaact gtcggcggca aaagtcgcca
    41101 gtctgcgtca gcgatttggc gaacatattc tggcggtgga aggtaacgtg acctgttatg
    41161 ccgattatca acgcgcggtc gatcagatcc tgactcgttc cggcaagctg gattgtttta
    41221 tcggcaatgc gggcatctgg gatcacaatg cctcactggt taatactccc gcagagacgc
    41281 tcgaaaccgg cttccacgag ctatttaacg tcaatgttct cggttacctg ctgggcgcaa
    41341 aagcctgcgc tccggcgtta atcgccagtg aaggcagcat gattttcaca ctgtcaaatg
    41401 ccgcctggta tcctggcggc ggtggcccgc tgtacaccgc cagtaaacat gccgcaaccg
    41461 gacttattcg ccaactggct tatgaactgg caccgaaagt gcgggtgaat ggcgtcggcc
    41521 cgtgtggtat ggccagcgac ctgcgcggcc cacaggcgct cgggcaaagt gaaacctcga
    41581 taatgcagtc tctgacgccg gagaaaattg ccgccatttt accgctgcaa tttttcccgc
    41641 aaccggcgga ttttacgggg ccgtatgtga tgttggcatc gcggcgcaat aatcgcgcat
    41701 taagcggtgt gatgatcaac gctgatgcgg gtttagcgat tcgcggcatt cgccacgtag
    41761 cggctgggct ggatctttaa ggaagcacga tgaaagaaaa aacgatcatt attgtcggtg
    41821 gcgggcaagc ggcggcaatg gctgcggcct cgctacgcca gcaagggttc accggtgagc
    41881 tgcatctgtt ttccgatgag cgacatcttc cttatgaacg acctccactc tcgaaatcca
    41941 tgttgctgga agattccccg cagttacagc aggtgttacc cgctaactgg tggcaggaaa
    42001 acaatgttca tctgcattcc ggtgtaacca tcaaaacgct gggtcgcgac acacgagagt
    42061 tagtgttaac caacggcgaa agctggcact gggatcagct ttttatagca accggcgcgg
    42121 cagctcgacc gctgccgttg cttgatgcac tgggagaacg ctgctttacc ctacgccatg
    42181 ccggtgatgc cgccagactg cgagaagttc tgcagcccga acggtcagtc gtgattgtcg
    42241 gtgccggaac tattggtctg gaactggctg ccagcgccac gcagcgcaga tgtaaggtga
    42301 cagtgattga actggcggca accgtcatgg gccgtaatgc accaccgccc gtgcaacgct
    42361 atcttttaca gcgccaccag caggctggtg tgcgcattct gctcaataat gccattgaac
    42421 atgtggtcga tggtgaaaaa gtagaactga cgctgcaaag tggggaaacg cttcaggctg
    42481 atgtggtgat ttacggtatt ggtatcagcg ccaacgagca actggctcgc gaggccaacc
    42541 ttgatactgc caatggcatt gtcattgatg aggcttgccg cacctgcgat cccgcgatct
    42601 ttgccggtgg cgatgtggca atcactcgtc ttgataatgg tgcactacac cgctgcgaaa
    42661 gctgggaaaa cgccaataac caggcgcaaa ttgccgctgc cgcaatgtta gggctaccgc
    42721 taccgctact gccgccgccg tggttctgga gcgatcagta cagtgataac ttacagttta
    42781 ttggcgatat gcgtggcgat gactggcttt gtcgtggcaa cccggaaact cagaaggcga
    42841 tttggtttaa tctgcaaaac ggcgtgctta tcggtgcggt cacgctgaat caggggcgtg
    42901 agattcgccc aattcgcaaa tggatccaga gcggcaaaac gtttgatgcg aaactgctga
    42961 tagatgagaa catcgcgctt aaatcactgt aaccaggata attagcgaat atctcaatgc
    43021 ctggggcgtg gcgaggtgca agagtgtgta ttacgtttaa atcacattat cttgcaaagg
    43081 gattggttat gaacacacta cgttattttg attttggagc tgcccgcccc gttttgttat
    43141 taattgcccg tatcgccgtg gtcttaattt tcattatttt tggttttccc aaaatgatgg
    43201 gctttgacgg tacggtccaa tatatggcct cgttgggcgc gccaatgccg atgctggcag
    43261 cgattattgc ggtagttatg gaagtgcccg ccgcgatatt aatcgtgctt ggctttttca
    43321 cccgtccgct ggcggtgctg tttattttct acacgctggg tacggcggtg attggtcacc
    43381 attactggga tatgaccggc gatgcggttg ggccaaatat gattaatttc tggaagaatg
    43441 tcagtatcgc tggcgcattc ctgctgctgg caattaccgg gccgggggca atttctctcg
    43501 atcggcgtta ggaaagatgc cggatgcggc gtgaacgcct tatccggcat ttaataaatt
    43561 acagccactc tacccgcaac gacatctctg aagttgttga ttcccctggt gctagcgcaa
    43621 tgagatcacc cccttccggg cgatgatgat catccggcgc atggctcatc ggttccagac
    43681 agaagaaatc aaacgcatat cctttatcaa acgcagggtc ggaaacaaag atgaaataac
    43741 acggtgcagg tggcgtcgtt tccatgatga tggcataacc ttcctgcggc tgctcgatgc
    43801 gggcctgacc attccatccg gcaaaaccat tgttcaccca ctggcgcggc aacggcgcgg
    43861 gctggttaaa atccagttcc tgcggtagct gctcgcaaaa ctcacccgcc agccactgct
    43921 cccgctccag ccagtaaccg ctcgcctgtg cctgaatccg cgtttgcggc gacaacggaa
    43981 aataaggatg ccagccggta ccaaatggca gcgtctctgc cccttgattg gtgacagaga
    44041 gcgtcaccgt cagcgtatcc gccgttaaat gaaacgcctg acttacccga tagtgataga
    44101 caccgctgcg atgttcatac accagacaca aactatcatc gctgtgcgag acacattgcc
    44161 actcgcccag ccagccatcg ccgtgtagat agtgcgcatc ccactcaaca ttcggttgca
    44221 gttgatactc acgcccctgc cagacaaaac gattgccgct cacccggttt gcaaatggca
    44281 ccagcggaaa acatgaggca tcggttgcca caccgctttt tttaccagga cgtaagagcg
    44341 gcgtcgtatc gcgccagaag ccttcgatta cgccgccctg gtcagaaacg tccagcttta
    44401 gcgacccgtg ggataaggta tagatggtca tccgctaact ccttaatccg ggaagttaat
    44461 cacaactttc ccgcatttgc cgctcgccat cagcgcatag gcatcacctg cctgttccag
    44521 cgagaagcga tgggtaatgg cgttacgcgg ccacagtttc cagtccgtca gatcatgggc
    44581 gcatttttcc atatggaaca gactggtcac ccaggagccg ataatccgcc gttgatggtg
    44641 catcagatcg gcgctgacct cgaactccac ttttccggtt tctccgatgt aaaccacccg
    44701 tccccagtca gcggtggatc gcagtgccag caagcgacct gcggcattac cagaacaatc
    44761 gagcgcaaca tccgcgccac cgtgggtgag ttcggcgata atctgcggca gaccttcggt
    44821 ggttgctaaa tagccgtgat ccatcacccc taactgtttt gccatcgcca gacgttccgg
    44881 cagcatatca acgccgatga tccgttttgc accgcgacct ttcgccagca tcatcgccat
    44941 catgccgact ggccccagac cgaccaccag cacgttatca ctgccggaaa cttcgccgcg
    45001 caaaatccct tcataggccg tgccaacgcc gcaactgata aacgcaccat cttcgtagct
    45061 cagcgcatcc ggcaggagga tcagatcttt ttcttccgcc agcagatatt cggcatgacc
    45121 gccgtcacgc tgccagccgt aagccgcttt tcctttgcca gtacaagaga taggaaagcc
    45181 gcggcggcag ttcgggcaaa aaccacagcc agaaatgtga tacaccagca cgcgatcacc
    45241 ctctttaaaa tggcgacagc cttgccccat cgccacaatc tgcccgcacg gttcatgacc
    45301 gttgataaat ccctggtata acggtttatc gggtgccgcc gccgtggcac ggtgttgatg
    45361 atagatatag tggacatcgc ttccgcaaat cccggaggat ttcattttga tcagtacctg
    45421 gttaatcccc ggcgtcggca ccgcaacttc ccgcagatcg acggttgaat ttcctggtaa
    45481 ataagctgcc agcatcgttt tcataaatcc ctcattaaca atacgattaa ttttcatccc
    45541 tgccgcaccc gcgccagggg cgttaaggtt tagcgtttcg ctttactgct tcgctgggtc
    45601 agcaagatat tcgccagcac cgccaccacg atgatgacgc cgcgtaccac ctgctggaaa
    45661 aaggagttaa taccgagcag caccagaccg ttaccgatta gcgtaatcac cagcacacca
    45721 agcaatgtac cgaacaggga accgcgaccg ccggaaagtg ccgtaccacc gaccacgacc
    45781 gcggcgatga cgtcaaactc cagaccgttt gcggcacctg cgttaccaga accgaggcgc
    45841 gccgccaaca aaatgccggt caccgccgct aataatcccg aaagggtaaa gataagaatg
    45901 cgcacccgac gaacgttgat gccgcacaac tgcgccgccg tagcattacc gccaacggca
    45961 aaaaccgagc gcccgaaggc ggttttgcgg ctgatgaaca caaacagcgc aaacaacaca
    46021 atcatgatca gcgcggatac cggcacaccg agaaattgtc cgcccagcca gtccagcacc
    46081 tcgttttcgt caatcggcac tggcagtgcg ttcgtcataa acagccccat tccgcgcagg
    46141 gcgctccaca gccccagtgt ggcaacgaaa cttggtacgt taaacacgcc gcgcagcacc
    46201 ccggccagcg ttcccatcag cgcgcctaac agcaacacca gcagacacgc caccgccagc
    46261 ggaacttcaa attgcagcaa aaatgccagg cacaccgaga caaaagccac catcggccca
    46321 acgctgacat caatttcacc ggagataata atcagcgtca tcgcccaggc ggcaatccca
    46381 atggtggcgg catcgcgcag cacgttcatc tggttattca atgagataaa gccaggcgcg
    46441 ttcagggaga agaccagata aagaatggcg attaccacca gcaaaccgat ctcattaata
    46501 tggcgactga caaattgttt gagcgagacg ctcttgccct gcggcaatga taatgacgaa
    46561 gcagacatct tcaggttcct cggtaaaatt catcagtgca cagacagaat ggcggacatc
    46621 agctcatcca cattgaccgg cgagtgaaac tcctgcgaga acgtaccgtg ctgtaacaac
    46681 aggatgcggt cgcacaccag cggcaactcc tccacttcac tggagataaa caccacgctt
    46741 tttccttcgg cagccagctc acggacaata cggtaaatct gctgtttggc ttcgatatcg
    46801 acgccgcgcg ttggctcgtc gagcaacaaa atctggctgg cggcatagac ccaacgaccg
    46861 atcaccactt tttgctgatt gccgccagaa agcgtgccga tgggcgtttc gctactggcg
    46921 gccttgaccg tcatccgctg catcacctct tcggtcaggc ggcggatggt ggaccattgc
    46981 agtacgccgt tggcgctgat tttttgccga ttggtcagca ctgtattttc gtcaacgccc
    47041 aaccagggaa tgatccccgc ttctttgcgg ttttctggcg tatagccaat gccgcgtttc
    47101 agcatgtcgc cgtaatcggg gcgcgtgatt ttctcgccgt tgataacaat ttcgccctgt
    47161 tcatactcct ccagcccaac aatcgccttc agcaattcac tgcgccctgc ccccagcagg
    47221 ccagcaatgc caagcacttc gccgcgacgt aacgtaaagc tgatatcctc cagcttgggc
    47281 ttatggcgta atgcacggac ttccagcacg gcctgatcca caatttcctg aggggctacc
    47341 ggcgcaatat caacgtgatc acgccctagc attagcgaca caatatgatg cgtggaggtg
    47401 ttttcgagca tcacatcgcc cgccacctga ccatcgcgca taacggtggc acaggaggca
    47461 atgcggcgga tttcttccat ccggtggctg acataaatca ccgccacgcc cagtgccgac
    47521 atctttttca ccgcactgat caccagttca acttctgcac tggcaagcga gctggtgggt
    47581 tcatcaagaa tgaccacgcg cggctcgccc ttcatcaccc gtgcaatttc caccagttgc
    47641 ttttgcgccg ggcttagcgt tgaaacaagt tgttcaggac taacgtcaac gcccagcgcc
    47701 tgtaagcaac gttgggcatc ctgcgccatt tgcaggtaat caatcatgcc gttgcggcga
    47761 ggccattgac caaggcagag gttttctgcc accgtcagcc cttccaccag acttaattcc
    47821 tgataaaccg cacgaacccc cagttcagcg gcacggcgag tcaacgtagc ttcgtcacct
    47881 tccagtcgcg tctcgccaat ccagatatca ccgctatccg ggcgttcgct gtcggtaagc
    47941 attcgaatga gagtcgattt gcccgcgccg tttttaccta acagcgcacg aacttcgcct
    48001 ttattgagcg tgaagttaac gttatccagc gcaacgacgc cggggtaacg cttatttcct
    48061 gccaccactt ttgctaccgg gactgcctct gttgccgtga acatagggac ctctgcgaat
    48121 cagcgattag ggcagaccat caacgtgcgt tgccagccac tgtttaccat cttccgtttt
    48181 ggtatagaga tcgattggca cctgaatcac tttttcaccg tcggcttgtt tattgataac
    48241 cttcaatgtt tgcgcgaaaa cagcattgcc cattttctta ccggaaatat ccactaccgc
    48301 tttcagcacc tgattgtttt ccagctcctg agcaatttcg gttgtcatat ccgaaccgaa
    48361 aacagcaatt tttccggcct gattttgatt acgtaccgct tttaccgcgc cgagtgtcgc
    48421 accgcccgac tcccccataa tggcgttgag atccggcgtg gagataatca gtttttcacc
    48481 aacggaaatc gctttatcta aaacagtccc ttcctgatta gcgacaattt gcgcgccggg
    48541 aacgcgggat tttaatactt cttcaaatcc tttacgtcgc tgcacacaaa cttcaaaggc
    48601 ttcgcaattg atgacggcaa ttttcggctg gtcaatttta ttggcaataa aataatcggc
    48661 ggcagcgtta cccagttttt taccaaattc cagtggatcg ccgaccagat acgccgagac
    48721 atatttatcg acaccctttt gattaataca ggtgttgtag caaatcaccg gaatacccgc
    48781 ttcactggcg cgacgaacgg tacggctact gccattttca gacactgccg ataaaataat
    48841 ggcatcgaca ttacgcgcca cgagggtatc aacaaaggta ctttctttcg aaatatcacc
    48901 ctgggcgtta gtttcaatta actgtacctg tactgaagaa tcttttgccg catcctgaac
    48961 gccctgacgc actccagcgt aatatccctg ggtatcaagg tatattgcac caatggtcat
    49021 ttctttatcc gcagccctgg caaatagtgc gctacctaat agcgtaccca ttaataatag
    49081 attacgggtt gttctcattt ttttaggcat agtgcctcct gtagggtttt tattaacaac
    49141 ggcttattct aattattttg tgatgagcgg cagcgcgtgc cgctcaaata ttacagttaa
    49201 tagggtttag tgaacgtagt taaaaataaa aggcatcacg gtggtgaata atgccgcttt
    49261 cggccaggcc ggattaatcg ccgtcaattc agcgcgagca gattcatatt gcgcaacctc
    49321 cccgagtccc gcggacgcca gcatcgccac catcaggcat ttttctttat gctgctgttg
    49381 taaatcgcca tacagcgaca acaggtcagg ttgtgaaaca gcaaagaaat ccgcctcaat
    49441 actggttttc gccatctctt gcgcccactg tttcatttca ctaaacagtt gctgtgcggt
    49501 ttgctgttca cccagcagtc gcagcgccat tccttgccag aagagataat caaccggctg
    49561 atcgttgtaa taactgtgga tgttaatggt gcgatcgccg gtcgccgcca gacgtaaaca
    49621 acgcatcgct tcagtttcat cgccctgcgc gttggcgcat atcgcctgcc agaaccagat
    49681 gtcgttatca gtttgccccg gtaaacggcc ttcgcttaaa ttctccggat aatgcagcgc
    49741 ggcatgaagc agttcgctgg cctgctgcgg ctctctggca tcaagatgct gccaggcgcg
    49801 taataactgg ttgaggataa actgactggt gaccttccct tccccgcctt cccacgggtg
    49861 gaatttgcgc gtggcgagaa tgtccgccgc tttgtctgcc tgacccgtga gatgccacaa
    49921 attgagcaac tctgctgtca tgtcgtcgcg tttcagcgca atttccagat tattttccag
    49981 acgcgccagt cgtttctccg gtgtggctcc gcttaactta tcaagcaaat cccgttcgaa
    50041 aagcagacgt gcatcctgcg gcgcaagctg ataagcatta tcaagataac gcgcggccag
    50101 ctcataatcg tgttgcttat tccacgcatg gatcgctaac ccgcgccagc cgtcggcaaa
    50161 ctccggcgac atctctacgc aacgttgcca taaggtaatg gctttgttgt agctacgctt
    50221 gttgtagtag aagcaggcca gtaaatgacg ggcaaaccag cactcttcaa tactctccag
    50281 cgccgccact tcttccagcg tattcgggaa acggacaaac tgcgggaaga catcaatggc
    50341 ttttgcgacc agttcgccgc gttcggcttt cggcaataaa ctcgcctgca ggtaaagcgg
    50401 cagcgtgcgc tggcagtcca gtgcgttcag catctctgcc gccagggtgg gcattcccca
    50461 gttaatcagt tgcccggcgg tcatcagagc gttaacgtcg cgtccctgac acagaccgcg
    50521 ccactgcgcg agagctgatt cgctacgacc atcgaaccag ttcagccacc acagagtggc
    50581 gttcagcgga taatcgcgca gcagtttctc gcgctgcaca cgcgcgttgt cctgacgacc
    50641 acttaacacc agcagcagat tatgcaggca aagcacttcc tgattggttg ggcaggcgcg
    50701 aagactttgt tggcaaaaat ccagaccagc gtcgaagtta ccattacgcg ccgccagtcg
    50761 tgccaggcca taatagccac cggctttgct gttgccgctc cagaccgcac gccagaaatc
    50821 ctcttcggct tgttgatatt gtccctgacg ttcgtaagca ctggcgcgaa tcaaactggc
    50881 ctgtccgcac tgcggatttt tgttcagcgc atgtgcgcgt ttcagcgcct gagtggcata
    50941 cgccaccgct tgcgggaaat cggcgcggtt atattccagc atcgccagcg ccaggttaca
    51001 gcggtaatcc agcggatcca gcaccacgcc gcgcaggtag taatcgaacg gtgaacggct
    51061 ggcgtgatga tattgctcca gatgctgacc gataaaccag gcttcatctg tactggtaat
    51121 gtcttgtgct gccagtggcg ctttggcgac gtccggcaac ggcaactctt gcggctgatg
    51181 ttcctgataa ctcagtacaa tattgccgtc ggcatcggag agctcaatgg tcagcctttc
    51241 aggattgata ccgtgcaaca cgccctggat ggcggtcgca ggcatcagtg ccacggcatc
    51301 atcaagtaac gcgttgcatt tgccgatttc gcggatcgcc aggcggtatc cgttcaacgg
    51361 agagatggca tacagccccc actcaatccc ccgcttacta cgctggagtt ttatcaccgc
    51421 atcgcgggag gcattttgca ccatgcccaa agaatgataa ggcaggaaat actgctcaaa
    51481 acgcttctct tcgtaagcat caagccaggt aaaatcgggc tggttatcgg caaaaatacc
    51541 ggtcatcagt tcgatatacg ggccgttatt gtcggtcaga ctcttatccc acgcctggcc
    51601 aaattcactg tgtccccaac tccactgttt tttacctggc gcgatatggt ggttggcaac
    51661 gtgcagcaaa ccgccatctt catcgtgaca ccacgcgccg acaaaatcgt actgcgattt
    51721 ttcggccata tatgaggttg gaacaggcac atttttatag cgagaaatgt ccactccggc
    51781 ggagtagtcc actttgtagt aagtgccggt ggcgatgggg aaagcggaga cggcccgttt
    51841 gccgtgatca aacaccgcag tcacatccgg tgggaaaacg ctctgatgcc cttccccccc
    51901 tttcactgcc gggttggccc accacaagaa atgacgcggc gtggcgttac cgttatagac
    51961 gcggctggcg atttccagcg ccgcccggtc agggcgcagg gtgaaacctg tcatcacctg
    52021 taaaccatgc atcggctccg tttcgcctac ccacaccgtc tgtgcaccgt cttcatgggc
    52081 ttcgagggtg aaatcaacgg gcataaaggt ggtcgggcga tgatgttgcg gccagttaaa
    52141 ctcaatccca ccggaaatcc acggccccag cagccccacc agcgcaggtt taatgacttc
    52201 attgtgataa acaaaatcgc gctgtttcac tttatcccac gcgcgatgca cccgaccgcc
    52261 cagttccggc aggatcatca ctttgatgta gtcgttttcc agccacaccg cctgccagga
    52321 tttcagggtt ttctgctcgc tcagcgtatc ggtcacgccg tagggataaa ccgcgccgga
    52381 cgatccctga taaacgcgat tttccaggaa catgggatgt atatcctgcg gcccggtttc
    52441 ataggtcggg atctcaacgc gctcttgcca cacttttact ggagtcatgg tgccctcaat
    52501 attaacaaga catactgaat taaaagattt gtggcagtgt attgaacaat ctggcaatgt
    52561 tttcgcggaa taatcacgca attaactaaa caaggtttag tgaagatgag agcctgcatt
    52621 aacaatcaac agattcgcca ccataacaaa tgcgtgattc tggaactgct gtaccggcaa
    52681 aagcgcgcca ataaatcaac gctggcccgg ctggcgcaaa tttcgattcc ggcagtcagt
    52741 aatattttgc aggaactgga aagcgaaaaa cgggtggtga atattgacga tgaaagccag
    52801 acgcgcgggc atagtagcgg tacatggctg attgcgccga aaggtgactg gacgctgtgc
    52861 ctgaacgtga cgcccaccag tattgagtgt caggttgcta atgcttgttt aagtccgaaa
    52921 ggtgaatttg agtatttaca gattgatgca ccgacgccgc aggcgctgct gtccgaaatc
    52981 gaaaaatgct ggcatcgcca ccgtaaattg tggccggacc gcaccatcaa tctggcgctg
    53041 gcaatccacg gtcaggttga tccggtgacc ggcgtgtcgc aaaccatgcc gcaagcgccg
    53101 tggacaacac cggttgaggt gaagtatctg ctggaagaga agctgggcat tcgggtgatg
    53161 gtcgataatg actgcgtgat gctggcgctg gcggagaaat ggcaaaataa ttcgcaggaa
    53221 cgggatttct gcgtgatcaa cgttgattac ggcattggct cgtcgttcgt gattaacgag
    53281 caaatttatc gcggcagttt gtatggtagc ggacagattg gtcacaccat cgttaatccg
    53341 gatggcgtcg tctgcgactg tggacgttac ggctgcctgg aaaccgtcgc ctcgttaagt
    53401 gcattaaaaa aacaggcgcg ggtatggcta aaatcacaac cggttaatac tcagcttgat
    53461 cctgaaaaac tgacaaccgc gcagttaatc gctgcctggc agagtggaga accgtggatc
    53521 accagctggg ttgatcgctc tgccaatgcc attggtttga gtctgtataa cttcctcaac
    53581 atcctcaata ttaatcagat ttggttgtac ggtcgcagtt gtgcctttgg tgagaactgg
    53641 cttaatacta ttattcgcca gacaggattt aacccgttcg accgcgacga aggaccgagc
    53701 gtgaaagcga cgcaaattgg cttcgggcaa ttaagccgcg cacaacaggt gctgggaatt
    53761 ggctatttgt atgttgaggc gcagttacga cagatttgat ggcgcgataa cgtagaaagg
    53821 cttcccgaag gaagcctttt ttcaacagat taaccgtttc gcttatgcgt aaaccgggta
    53881 acgtgcgcag atgtcgagaa ctttaccttt gatgcgctcg ataacggctt catcattgat
    53941 gctgtccagc acgtcacaca tccagccagc cagttctttc gcttctgctt ctttgaagcc
    54001 gcgacgcgta attgccggag tacccacgcg aataccggag gtcacaaacg ggctcttcgg
    54061 atcgttcggt acgctgtttt tgttgacggt gatgttagca cggcccagag cggcgtctgc
    54121 ttctttaccg gtcaggtttt tatcaaccag atcaaccagg aacaggtggt tatcagtgcc
    54181 gccggaaacc actttgtagc cgcgctcgag gaacacttct accatcgctt tagcgttttt
    54241 cgcgacctgc tgctggtaag ttttgaactc aggctccatc gcttctttca gagcaaccgc
    54301 tttaccggcg attacgtgca tcaacggacc gccctgacca ccagggaaaa cggcagagtt
    54361 cagttttttg tacagctctt cgctaccacc tttcgccagg atcaggccgc cgcgcggacc
    54421 cgccagggtt ttgtgagtgg tggtagtaac aacgtgagca tgaggaaccg ggttcgggta
    54481 gacgccagca gcaaccaggc ccgcaacgtg cgccatatca acgaacaggt aagcaccgat
    54541 gctgtcagcg atttcacgca ttttcgccca gtccaccacg ccggaatatg cagagaaacc
    54601 accgataatc attttcggct tgtgttcttt ggcttgtttt tccagatcgg cgtagtcgat
    54661 atgaccggta gcatcgatac cgtaaggaac gatgttgtac agtttaccgg agaagttaac
    54721 cggagaaccg tgagtcaggt gaccgccatg cgccaggttc atacccagaa cggtatcacc
    54781 tggttccagc agcgcggtgt agaccgcaaa gttagcctgg gagccggagt gcggctggac
    54841 gttagcgtag tcagcgccga acagttcttt cgcacgatcg atcgccagtt gttcaacgat
    54901 atcaacatac tcgcaaccgc cgtagtagcg tttgcccgga taaccttcag catatttgtt
    54961 ggtcagctga gaaccctgcg cctgcattac gcgcgggctg gtgtagtttt cggaggcgat
    55021 cagttcgatg tgctcttcct gacgtacttt ttcctgctcc atagcctgcc acagttcggc
    55081 atcataatcg gcaatgttca tttcacgctt taacatccgc atctcctgac tcagctaaca
    55141 ataaaatttt tggcctttat aggcggtcct gttggacaac ggcgaacagt ataaccgaat
    55201 cattgtgcga taacaggtct tgacaaagga atttacgcaa acgattacct tcaggctaca
    55261 caaggctttg gagaataaag agcttgcaac cggaaacgga tttcttttca ggtttgtgat
    55321 gcaaattttt cacttcatca cattctttct gaaaaacacc aaagaaccat ttacattgca
    55381 gggctatttt ttataagatg catttgagat acatcaatta agatgcaaaa aaaggaagac
    55441 catatgcttg acgctcaaac catcgctaca gtaaaagcca ccatcccttt actggtggaa
    55501 acggggccaa agttaaccgc ccatttctac gaccgtatgt ttactcataa cccagaactc
    55561 aaagaaattt ttaacatgag taaccagcgt aatggcgatc aacgtgaagc cctgtttaac
    55621 gctattgccg cctacgccag taatattgaa aacctgcctg cgctgctgcc agcggtagaa
    55681 aaaatcgcgc agaagcacac cagtttccag atcaaaccgg aacagtacaa catcgtcggc
    55741 gaacacctgt tggcgacgct ggacgaaatg ttcagcccgg gccaggaagt gctggacgcg
    55801 tggggtaaag cctatggtgt actggctaat gtatttatca atcgcgaggc ggaaatctat
    55861 aacgaaaacg ccagcaaagc cggtggttgg gaaggtactc gcgatttccg cattgtggct
    55921 aaaacaccgc gcagcgcgct tatcaccagc ttcgaactgg agccggtcga cggtggcgca
    55981 gtggcagaat accgtccggg gcaatatctc ggcgtctggc tgaagccgga aggtttcccg
    56041 catcaggaaa ttcgtcagta ctctttgact cgcaaaccgg atggcaaagg ctatcgtatt
    56101 gcggtgaaac gcgaagaggg tgggcaggta tccaactggt tgcacaatca cgccaatgtt
    56161 ggcgatgtcg tgaaactggt cgctccggca ggtgatttct ttatggctgt cgcagatgac
    56221 acaccagtga cgttaatctc tgccggtgtt ggtcaaacgc caatgctggc aatgctcgac
    56281 acgctggcaa aagcaggcca cacagcacaa gtgaactggt tccatgcggc agaaaatggc
    56341 gatgttcacg cctttgctga tgaagttaag gaactggggc agtcactgcc gcgctttacc
    56401 gcgcacacct ggtatcgtca gccgagcgaa gccgatcgcg ctaaaggtca gtttgatagc
    56461 gaaggtctga tggatttgag caaactggaa ggtgcgttca gcgatccgac aatgcagttc
    56521 tatctctgcg gcccggttgg cttcatgcag tttaccgcga aacagttagt ggatctgggc
    56581 gtgaagcagg aaaacattca ttacgaatgc tttggcccgc ataaggtgct gtaatttgat
    56641 gttgccggat ggaaacatcc ggcaaccctt gacgcggttt aaattgccgc gtcgtcctct
    56701 tcaccggtac ggatgcgaat gacccgtgcc acgtcgaaga cgaagatttt accgtcaccg
    56761 attttgccgg tttgcgccgt gcgaataatg gtatcaacac aggtatcgac aatgtcgtcc
    56821 ggtacgacaa tctcaatttt cactttcggc agaaaatcca ccatatactc cgcgccgcgg
    56881 tacagctcgg tatggccttt ctggcggcca aaacctttca cctcagtgac cgtcatgccg
    56941 gtaatgccga cttcagccag tgcttcgcga acatcgtcca gcttgaaggg tttaataatc
    57001 gcatcaatct ttttcatgct attccttgaa aaggtcgcct gtcttttgat ctgctaaacg
    57061 taacacataa cgccaattca ttccttgaaa tcgtttgcat ccagctcgtg tcgggaaagc
    57121 agtttataaa attctgtccg gttgcgcccc gccattctcg ccgcgtgtgt gacgttgcct
    57181 ttggtgattt gcagcagctt acgcaaatag ttgagttcaa actgattacg tgcctcaaca
    57241 aaggttggca gcgccgtatt ttcaccctcc agcgcctgct ctactaatgc atcgctaata
    57301 accggagatg aggtcagcgc cacgcactgt tcaatcacgt tgaccaactg gcgcacatta
    57361 cccggccagc tagcggtcat caggcgtttc atcgcatcag tggagaacgc gcggacaaac
    57421 ggtttatgtc gctctgccgc ctggcgcaac aggtgatttg ccagaagcgg aatgtcttct
    57481 gtgcgctctg ccagagccgg aattttcagg ctgacaacgt tgaggcggta atagaggtct
    57541 tcacggaatt ccccgcgcgc catcgctttt ggcaggtcgc ggtgagtggc ggaaataatc
    57601 cgcacgtcga tatcaatatc gcggttactg cccagcgggc gcactttacg ctcctgcaac
    57661 acgcgcagca gtttgacctg caacggtgcg ggcatatcgc caatctcatc gagaaatagc
    57721 gtaccgcctt ccgccgcctg gaataaacct tcgcgattgc tgacagcgcc agtaaacgcg
    57781 ccacgcgcat gaccaaacag ctccgactcc agcaattgct cgggcaatgc gccacagtta
    57841 atagcaataa atggtttgct attgcgcggg ctggcgttgt ggatagcctg ggcgaaaatc
    57901 tctttcccgg tgccgctctg gccgttaatc aaaacgctaa cgtctgattg cgccaccagc
    57961 cgcgcctgtt ccagcaaacg cagcatcagc gggctgcggg tgacaatcgc ctcgcgccag
    58021 cgttcatcgg tggctggcgc ggattgttcc agcgcatcgt caattgcctg atatagcgca
    58081 tctttatcga caggcttggt gaggaaactg aacacgccct gctgtgttgc agcaacggca
    58141 tcgggaatag aaccatgcgc ggtaagaata attactggca ttcccggctg cactttctga
    58201 atttcagcaa acagctgcat accgtccatt tcatccatcc gcaggtcgct gatgactaaa
    58261 tctacttttt cgcgattcag tacccgtaat ccttcagcgc cactttccgc cgtgaccacg
    58321 ctgtagcctt cgctggtcag gcgcaggcca agcagtttca gcaatcccgg atcgtcatcg
    58381 accaataata aatgcgcagg tttatggctc atcaggagtg acctcatggg tggatggcgc
    58441 gggcttttca ccctcgtgcg gcgtatccgg cgagaaattt ccagccggtt tgcgggtcga
    58501 gagctggcgt tcaatatcag tcaggttttc cagcttgcgg gtggtgagtt ccagttgctg
    58561 ctgtagaacg tgatgttgct ggcgcaatgt atccagctcg ctgtcgctgg cctgctggag
    58621 cttgccgtag cgttggcgct cttccgccag ttgcagttgc agcgcctgac cgtcgcgcca
    58681 gagttgatac agagggcgaa cctgtgccgg gatctcggta cttagcgcat caatgcgcgc
    58741 gaccagctgg cggcgctcat acggcgtaat tttggcgtcg gcgagcaaaa tcccttgttt
    58801 aaaggtattt tgccagctgc cgtcgtcata ttggcgagct tgctgacgtg actgcgcagg
    58861 cattaaacga tcagcacaat ccatcgcccg cagccagtaa agcggattgg tttcggttga
    58921 tttaccttgc agcgcccaga tgtcgctaca ttcagtagaa agatagtcag ccagttgata
    58981 aaccggaatt ttttcttctg ctggcgtatc aatggctggc ttattgtgac tctgcacgca
    59041 acccagcaat gccagacatg gcagccctgc cagccacagc cgtcggggca ttaatcgttg
    59101 aaaaatgtgt cgcatattca ccagacttaa tgcctatccc agtgggcgta attattgcag
    59161 gcagtttgga catggacagc gcggagaaac cggagcgtac acgtcgtacg tgaggatttc
    59221 gagcactgcc cggggccgaa ctggcaaata aaatagcctg atgggatagg ctgttagcat
    59281 tatttcgtgt ttttcgacga cggcaattca atgcggaaac aaacgtcttg cccgctctcg
    59341 tcgaccagat acagttcccc ttgcatacgg cgaatacaat ccctggcaat gcttaatccc
    59401 agaccgctgc ccttcaccgc cccttttcgc tggtggcttc cttgaaaaaa gggttcgaag
    59461 atcatggcgc gttcctcttg cggaatgggt gtgcctgtat tgatgacatc aatataaacc
    59521 cgtgcaccat gtaagctgct gcgaaggcaa atgttaccgg attcagcccc gtagtgcacc
    59581 gcattggagt aaagattatc cagtacgctc atcagcagca ttggctccgc caggcaagct
    59641 gttgctttga gatcgacgtc ggtatgcatc attttagccc gtgcgggcag gctatgagca
    59701 gaaactactg tctccaccag cggtgctaac tcaacattct ccagttccac cgcactgtcc
    59761 gcctgtttac ggttgtaatc aagcagttgt tcgatcagtt tttgcaaatt gcggctgctg
    59821 ctatcaagaa tgctcaccac ctctttttgc tctggcgtaa gcggcccgac aacctggtca
    59881 gccagtaatt cagtgccctc gcgcatactc gccagtggcg tttttaattc atgagataaa
    59941 tgtcttaaaa attgatggcg ttgggattcc agccatgaca ggcgctcact taaccaaaga
    60001 atacgttgcc caaccgagcg taactcgctc ggtccactga acgaaacgct attgcccaga
    60061 gaacgccctt cccccagacg gttgatcatg cgctcgatat ttttcaccgg cccgataatc
    60121 atccgcgtga aaagtagcac catcaccaga ctcaccagaa atagcaccag cgattgccaa
    60181 ccaaaatatt gcccacgttc ggcgatttca cgctgaagtt gctgcccacg agagaacacc
    60241 actgtgcgcg tggcctgcac catttcggta ttggcactgg caaaggcttc aagacgcgcg
    60301 gcggcggcag catcgggacc actgttgtta cactgaagtt gggccagatt gttcaaatcc
    60361 tgacgcaatg cctggtaaag tttatcgtct ggcagtacgc ctgcgtgggc atcgagcatt
    60421 tcgctgtaac gcttgcgctg gctttgataa accttcgcaa gcgtagggtc gtccagcacg
    60481 caatactgac ggtagctacg ctccatctcc agcgccgcgt tggtcatcgc ttcactgcgc
    60541 cgggcgtcaa taagcgtggt gcggttaacc agcgccgcct gatcgctaag cgcattcagg
    60601 ctttgccagg cttgccatgc cagcaccaac aagggcagca gaattagcaa aaatgccagc
    60661 attaccagtt gtcgtaatga gcggggaaaa acgggccagc gtttcaaggt gttactctcg
    60721 tcagacgcga atagcctgat gctaaccgag gggaagttca gatacaacaa agccgggaat
    60781 tacccggctt tgttatggaa taaggcggtg cctaactcga cgtttcgccc gatggttgat
    60841 atagctacgc tgatatcaga agttggacgg caggcacctt gttgtgcgtc attcgaattt
    60901 tatgtagcac gtcccgaagg ggctgacata agtcggtgaa tgagccactg cttactatta
    60961 tgcagtaact gtgccaataa agaaaatagt ttggtaacgt actgattata cgttgctttg
    61021 agggatttat ggcttcttcg ctatttgaat gtaaatcagc cactgtgtcg ctaaaaagag
    61081 acaacttaag ataaacttat tagattacaa taaaatcaat catttaagtg tcgccagaaa
    61141 gcgacacggt aaaccaccca ttgtcgtgat ttacagacac aaaaaagctc ccggagttgg
    61201 gagcttatga tagtggttgg tgcttaatgc cgcatccggg ctgcaaaacc aatgaactga
    61261 tgaattaccc caactgctta cgcgcattgc ggaaaatgcg catccatggg ccatcctcgc
    61321 cccagttttc cggatgccag gagttgctga cagtacggaa aacacgttcc gggtgcggca
    61381 tcataatggt gactcgacca ctttcagtcg tgactgccgt aataccgttc ggtgaaccgt
    61441 tcgggttagc cgggtaggtt tcagtgactt tgccgaagtt atcgacatag cgcagtgcca
    61501 ccagcacttt gctttccagt gccgccagat gcgccgcatc acgcacttcc acgcgccctt
    61561 caccatgaga gacagcaatc ggcatttgcg agcccaccat cccctgcaac agcagagacg
    61621 ggctttgggt tacttcaacc aggctgaaac gcgcttcaaa gcgatcggag gtattgcgca
    61681 caaaacgtgg ccacaactca ctacctggga tcagttcgcg caggttagac atcatctggc
    61741 aaccgttaca tacccccagc gccagcgttt gcggacggtg gaagaaggtt gcaaactcat
    61801 cgcgtacacg gtcattgaac aggattgact tcgcccatcc ttcaccggca cccagcacat
    61861 caccgtagga gaaaccaccg cacgcgacta gggcgtggaa atcttccagg cccgtgcgtc
    61921 cagccagcag gtcgctcata tgcacgtcga tagcatcaaa gcctgcacgg tggaaagctg
    61981 ccgccatttc aacatgcgag ttcacgccct gctcacgcag tacggcaact ttcggacgtg
    62041 cgccagtggc aatatacggt gctgccacat cttcgttgat atcgaacgac agttttacat
    62101 tcaggcccgg atcggcgtcg ttagatttcg cctgatgctc ctgatcggca cactccgggt
    62161 tgtcacgcag gcgctgcatc tgccaggtag tttctgccca ccagacacgc aacgtggtgc
    62221 ggctttcgct gaatacagtc tgcccgttgg cggtaatcac aaaacggtca ccggaaaccg
    62281 cctgccctac ataatggaca caatcagcaa gcccatgctg tgccagtacg gactcgaccg
    62341 cttcacggtc agcggcacga acctgaatca ccgcacccag ttcttcgtta aacaacgccg
    62401 ccaggcgatc gtcgcccaga gaggcaatat ccgcgttaat gccgcaatga ccagcaaagg
    62461 ccatttccgc cagcgttacc agcaggccgc catcagagcg gtcgtggtat gcgagcagct
    62521 tacgctgtgc aaccagcgcc tgaatcgcgt catagaagcc tttcagttgc gcgacgtcgc
    62581 gtacatctgc cggtttgtcg ccaagctgac gataaacctg cgccagcgcc gttgcgccca
    62641 gcgcgttatt gcctttgccc aaatcaatca gcagcagtgc gttatcttcg gtagaaagct
    62701 gtggcgtgat ggtgtgacgt acatcttcca cgcgggcaaa tgcggaaatc accagcgaca
    62761 gcggcgacgt catttcgcgc tcttcgttac cttcctgcca gcgggttttc atcgacatag
    62821 agtctttacc caccgggatc gtaagaccca gcgccggaca aagctcttca cccacggctt
    62881 taacggcttc atacaggccc gcatcttcac cagggtggcc tgccgccgcc atccagttgg
    62941 cggaaagttt aatgcgtttg atatcaccaa tctgtgttgc ggcaatattg gttaacgctt
    63001 caccgaccgc cagacgggca gaggcggcga aatccagcag cgcaaccggc gcacgctcgc
    63061 caatcgccat cgcttcaccg tagtagctgt cgaggctggc ggtagtgacc gcgcagttag
    63121 cgaccggcac ctgccacggc cccaccatct gatcgcgcgc taccatgccg gttacgctgc
    63181 ggtcgccaat ggtcaccagg aaggtttttt ccgccacagt cggcagatgc agcacacgtt
    63241 tcaccgcatc tgcaatggtg atcccttcac gggccagcgc gtcgcctttc gctttcagcg
    63301 tttgtacatc gcgggtcatc ttcggcgttt taccaagcag gacgtccagc ggcagatcga
    63361 tcggctgatt atcaaaatga cgatcgtgca gagaaagatg cagttcttcg gtcgcttcac
    63421 caatcaccgc gtagggtgcg cgttcacgct tacacagttc gtcaaacagc ggtaattgat
    63481 cggcagcaac cgccagcacg tagcgttcct gggattcgtt acaccagatt tccagcgggc
    63541 tcatgcccgg ttcgtcgctt agaatatcgc gcagttcaaa tttaccgccg cgtccgccgt
    63601 cgctcaccag ctccggcatc gcgttagaaa gaccgcccgc accgacgtcg tggataaaca
    63661 gaattgggtt agcatcacca agctgccagc aacggtcgat cacttcctgg cagcgacgct
    63721 ccatctccgg gttgtcgcgc tgtacggaag caaagtcgag gtcggcatca gactgacctg
    63781 acgccatgga agacgccgct ccgccgccaa gaccgatgtt cattgccggg ccgccgagaa
    63841 cgaccagctt cgcaccgacg ttgatctcgc ctttttgtac gtgatcggcg cgaatgttgc
    63901 cgatcccgcc cgccagcatg atcggtttgt gataaccgcg cagctcttcg ccgttgtggc
    63961 tgttcacttt ttcttcataa gtacggaagt agccattcag tgccggacga ccaaattcgt
    64021 tgttaaacgc cgcgccgccc agcgggcctt cggtcatgat gtccagcgcg gtgacaatgc
    64081 gctcaggctt accgaaatct tcttcccacg gctgttcgaa gccaggaatt cgcaggttgg
    64141 aaacggagaa accaaccaaa cccgctttcg gcttcgcgcc gcgcccggtg gcaccttcat
    64201 cgcggatttc accgccggaa ccggtcgccg cgcccggcca cggagaaatc gccgtcgggt
    64261 ggttgtgagt ttcgactttc atcagaatat gcgccggttc ctgatggaaa tcgtagcggc
    64321 ccgtttcgtg gtcagcaaag tagcggccca cttcagaacc ttccattacg gcggcgttat
    64381 ctttataagc agagagaacg taatctggcg tggtttcgaa ggtgtttttg atcatcttaa
    64441 acagcgattt cggctgttgc tcaccgtcga taacccaatc ggcgttaaaa atcttgtggc
    64501 ggcagtgctc ggagttcgcc tgggcgaaca tatacagctc gatgtcgttc gggttacgac
    64561 caagctttgt gaaagcatcc tgcagatagt caatttcatc ttccgccagt gccaggccaa
    64621 gacgcaggtt agcgtcgatt agcgcctgac ggccctgccc cagcaaatca acgctggtga
    64681 ccggagtcgg ttgatggtgt gcaaacagct gctctgcatc atctaaagca aaaaagaccg
    64741 tttccatcat gcggtcgtgc agttcagcgg taacctgctg ccattgttca ttggtcagcg
    64801 taccggcttc tatatagtaa gcaacgccgc gctcaaggcg gtttacctgt tgtagcccgc
    64861 agttatgggc aatatcggtc gctttcgaag accagggaga gatggtgcca ggacgcgggg
    64921 tcaccagcag gagtttgcct tgcggggcgt ggctggcgag tgccgggcca tatttcagca
    64981 ggcgttcaag ttgtgcgtgc tcatcatcgt ttaacggcgc attgaggtca gcaaaatgga
    65041 catactcggc gtaaatattg tgaaccggga gcctggcagc ctgaaaacgt gccagcagtt
    65101 tgttgattcg gaatgccgac agtgcaggcg aaccacgcag aatttccatc ataagtctct
    65161 cgtcttctaa gctttcggtg tacccaaggg ggggaaacgg gcgtcattat aaagaatctg
    65221 atgcgctgac gaaaccgttt gcgtggaaat aaaatcacca tcgtgaatta gcaacgcgtg
    65281 ccgccaatgg ctgtaataag ttgccatctg gcgcaggttt acgcaaaatg ccgctcattt
    65341 atgagtaaac ctttcactat tattacgttt tttcaagctg ggacgcgcac gacacagaga
    65401 attaactaat tgaaaaaatt aaagattaat tatctgttca tcggcattct ggcactgctg
    65461 ctcgcggtcg ctctctggcc atccattccc tggtttggta aagccgacaa ccgtatcgcc
    65521 gccattcaag cgcggggaga gttgcgtgtg agcaccattc atactcccct gacttataac
    65581 gaaatcaacg ggaaaccttt tggcctggat tacgaactgg cgaaacagtt tgccgattac
    65641 ctcggcgtaa aactgaaagt gaccgtgcgg cagaatatca gccagctgtt tgacgacctt
    65701 gataatggta acgccgacct gctggcggca ggactggtct ataacagtga gcgggtaaaa
    65761 aattatcagc ctggccctac ctattattcc gtgtcacaac aactggttta taaagtgggt
    65821 cagtatcgcc cacgtacgct gggcaacctg acggcggagc aactcaccgt tgcaccgggt
    65881 catgtggtgg ttaacgatct ccagaccctg aaagaaacaa aattcccgga attaagctgg
    65941 aaggtagacg acaaaaaagg ctctgcggaa ttaatggaag atgtcatcga aggaaaactc
    66001 gattacacca ttgctgattc tgtcgccatc agcctgtttc agcgcgttca cccggagctc
    66061 gccgtagcgc tcgatatcac cgatgaacaa ccggtgactt ggtttagccc gttagatggc
    66121 gataataccc tttccgccgc cctgctcgac ttcttcaacg aaatgaatga agacggtacg
    66181 ctggcacgca ttgaagagaa atacctgggg catggcgatg attttgatta cgtcgatacg
    66241 cgcacatttt tacgcgccgt cgatgcggta ctgccgcagt taaagcccct gtttgagaaa
    66301 tacgccgaag aaattgactg gcgtttgctg gccgctattg cttatcagga atcgcactgg
    66361 gatgcacagg ccacttcacc gacgggtgtg cgcggcatga tgatgttaac caaaaatacc
    66421 gcgcaaagcc tcggcattac ggatcgtacc gatgccgaac agagcatcag cggtggcgtg
    66481 cgttatttgc aggatatgat gagtaaagtg ccggaaagtg tgccggagaa cgagcggatc
    66541 tggtttgccc tcgctgcgta caatatgggc tatgcgcata tgctggatgc ccgcgccctg
    66601 acggcaaaaa ccaaagggaa tcctgacagt tgggctgacg taaaacagcg tctgccttta
    66661 cttagccaga aaccctatta cagcaagctg acttacggct acgctcgtgg acatgaagcc
    66721 tacgcttatg tcgaaaatat tcgtaagtat cagattagcc tggtgggtta tctgcaagag
    66781 aaagagaagc aggctacaga agcggcgatg caactggcgc aggattatcc ggcggtatcg
    66841 cctacggagt tgggcaaaga gaaatttcct tttctctcgt ttctttccca gtcgtcatca
    66901 aactatttga cccattctcc ctctctgctg ttttccagga aagggagtga agagaaacaa
    66961 aattaatccg tcgaggattg cgcttttttc tgcgctttaa tttcctggcg gcgcatgcga
    67021 aagaagtcac tgagcaacgc cgcgcactca tccgccagta ttccttccgt aatttccact
    67081 cggtgattca tacccggatg atgcagcaca tccattaaag atcccgcagc gccagttttc
    67141 gcgtcacgcg caccaaagac cacgcgacca atgcgactgt ggatcatcgc tccggcacac
    67201 attacacatg gttcaagcgt gacatacaac gtggcgtcga tcagacgata attttgcatc
    67261 accagaccac cctgccgcag ggccatgatt tctgcatgtg cggtgggatc atggcgacca
    67321 atcgggcggt tccagccttc gccgattacc cgattgttat gcactaatac cgcgccgacc
    67381 ggcacttccc gctcatccca ggcacgtttc gccagcgtca gcgcgtgacg catccagtat
    67441 tcgtggctaa attcgacttc agacaaaaag aaaactccgg ttataaaagc gcggcgcatt
    67501 atacacggac gctatgcttt actctattcc agttgctgga gttcaccgcg cggggttact
    67561 cgccaacgat gctgacagaa ataaagcagc ggattgtcct gattactgtc gctatagcca
    67621 ctgtacagcc gcagcggagt gccgattttg cgctccagtt gtgcgacctt ttcatgtccc
    67681 agacaacgca tcgtcaatac ccaaccacca tagccacgct gaatttggct ggcgataaga
    67741 ttaacccgcg gcagccaggg cgtatcgaaa taaaccgctt caaccagcgg ctgcggagag
    67801 ccggtaatca accagatatc agcatcggaa cttaacaggt aggtggttaa tcgctcctga
    67861 accagcggaa aggcggtaac attgtcgcga aaccagcgca cgaaatcggc ctgcaacgtc
    67921 tgtaaacgtg cttcgctgtg accaaaagtg cacccccaca gaagcagact catcggccag
    67981 cgtgccgcac gaccttttat caataacgca atggctataa tcggtaacaa cggcaggaca
    68041 agtaacgcat tcagcggttg gcgacgcagt aaatagcgca gaaaactgcc gaacatatcc
    68101 tgctgatgca atgttccatc taagtcaaaa aacaccacac gacgctcgtg agttgccaaa
    68161 ccatactcct ctggactgaa acatcctgat taattttcgc tactacatag cctaacagat
    68221 agatcatcac ttttccggca acaatcgacc gcatcatggc taactggaat ttttaattca
    68281 tggcaattag cggcaatgga atataaaatt cactcgcgtg tctctcatat tgcctgttgt
    68341 cgccactatt tcatcgcgag gaaagatatg aacggcttac ttcgtatccg tcagcgttac
    68401 caggggcttg cccagagtga taaaaaactg gcggattatc tgctgctaca acctgatacg
    68461 gcgcgccatt taagctctca gcaactggcc aacgaagccg gagtcagtca gtccagcgtc
    68521 gtgaagttcg cgcaaaaact cggctataaa ggttttccgg cgcttaagtt ggcgttgagt
    68581 gaagcgctgg caagccagcc ggaatcgccc tccgtgccca ttcataacca aatccgcggt
    68641 gatgatccgt tacggctggt cggcgaaaaa ctgattaaag aaaatactgc cgctatgtac
    68701 gcaacgctaa acgttaatag tgaagagaaa ctgcatgaat gcgtaacaat gttgcgctct
    68761 gcgcggcgga taattctgac cggtattggc gcttcgggtc tggtggcgca aaactttgcc
    68821 tggaagctga tgaagattgg cttcaatgct gccgcagtgc gcgatatgca tgcgctactc
    68881 gcaacagtac aggcgtcgtc ccctgacgat ctgttattag ccatttccta caccggtgta
    68941 cgacgcgagt taaacctggc ggcagatgag atgctgcgag tgggcggaaa agtgctggcg
    69001 attaccggct ttactccgaa tgccctgcaa cagcgtgctt ctcattgcct gtataccatt
    69061 gccgaagaac aagcgacaaa cagcgcttca atctctgctt gtcacgctca gggaatgtta
    69121 acggatttgc tgttcattgc gctgattcag caggatctgg aactggcacc agaacgtatt
    69181 cgtcatagtg aagcgctggt gaaaaaactg gtctgagtaa agaatgcgcg tataatgccc
    69241 gcccggtttg tgttgttttg agagtttcct tatggcgttg ttaatcacta aaaaatgcat
    69301 caattgtgat atgtgtgaac ccgaatgccc gaatgaggcg atttcaatgg gagatcatat
    69361 ctacgagatt aacagcgata agtgtaccga atgcgtaggg cactacgaga caccaacctg
    69421 ccagaaggtg tgcccgatcc ccaatactat tgtgaaagat ccggcgcatg tcgagacaga
    69481 agaacagttg tgggataaat ttgtgctgat gcaccacgcg gataaaattt aatattctac
    69541 tctggaagta gagtattaat tatattactg ggaagcctta acgccattat atttatttaa
    69601 ttgatgacat tagcataatc attcactaag ttaatttata tagtatctgc caagacactt
    69661 atttatagtt attaaaggcg cgtccgattg gttcaccgga cgcaccttaa gtacgtttcc
    69721 ttgtgttata agaacagaag gatcagctgt aaaacagcaa tgatgatttt gatgacccgt
    69781 ttaatcaggt atcggcaatc agtcattcgt ttttccttaa acaaggaatg cagccgttca
    69841 gattagccct tacatctccc caaaactgaa cgtgcgagtt attgagggtg catgctgcac
    69901 tccacaccag agctttgacg acaccactcg tttcaatggg ggaattctgt ggcatggtgt
    69961 aaagcacagc aaaatcttca ataacgaagc caattttaat gtacttacga attggcggtc
    70021 acgttaatct ttccatcaat attgctttct tcgtaaaggc tcgagttttt atgctaaaga
    70081 ttgcaagttg cttgtaaaag ataagtacac tgatccataa tcgctgttgt tgagggtgca
    70141 tgctgcacaa aattaaagtt aaaaagtaaa acccccgttc cttaccagtt cgggggtttt
    70201 actttttaaa gagaacggta ttattttaac tttcaataat taccgtggca caagcatagt
    70261 ggcgttcatc tgccagcgtt acatgcatat ttacaacgcc gagcttttcc gccagtttta
    70321 atgcctcacc ccatagccgt agccgtggtt tgccgagctc atcattgaat acttcaaatt
    70381 gattaaacgc cagaccattg cggatcccgg tgccaaacgc ttttgctgcg gcttctttca
    70441 cagcaaaacg cttcgccaga aaacgcaccg gctggtggtg cgttttccag attgcccatt
    70501 cgttatcgct taatacgcgg cgtgccaggc gctcaccgga tcgggcgatc actgcttcta
    70561 tgcgagcgat ctctacgata tccgtgccta aacctaatat tgccattagc cacgcgcttc
    70621 cagcatcaga cgcttcattt ctgccaccgc atctttcagt ccggtcatca ctgcacgacc
    70681 aataatggca tgaccgatat tcagttcatg catctcaggg atggcggcaa tagctttcac
    70741 gttgtgatag gtcagaccgt gaccagcgtt aactttcaga ccgagactag cggcaaaggt
    70801 cgcggctttg gcgatacgcg ccagctcttg cgcctgttcg gcgtcagttt tggcatcagc
    70861 atagcaaccg gtgtggatct cgataaacgg tgcgccaacc tctgccgcag ctttgatctg
    70921 ctcttcatcg gcgtcgataa agagagaaac ctggatcccg gcatctgcca gacgtttgca
    70981 ggcatcgcgc attttgtcac gctgccctgc gacgtccagg ccgccttcgg ttgttacttc
    71041 ctgacgcttt tccggtacca gacagcaaaa atgtggcttc gtctcaacgg cgatcgccag
    71101 catctcttcg gtcaccgcca tctccagatt catgcgggta tccagcgtct gacgcaggat
    71161 gcgcacatca cggtcagtaa tgtggcggcg atcttcacgt aaatgcacgg taatgccgtc
    71221 cgctcccgcc tgctcagcaa taaacgccgc ctgcaccgga tccgggtaag cagtaccgcg
    71281 tgcgttacgc agcgttgcaa tatggtcaat gttgacgcct aacagtaatt cagccatgac
    71341 aatcctcatc attcataatg tgttttcacc gttcgcttag gcataaactg ccggaacagt
    71401 tccctgctct ttaatggttt accgccaaga tacggcttaa gcgccatgcg ggtaaagcgt
    71461 ttcgcggcgc gtagtgtgtc tgcgtcagga aattcccgcg cgtttaacgc ttttaactgc
    71521 cttccggtga acgttttatt gtcgataacg acgcttgcga taaacccttt ttcttcgcga
    71581 taacgatacg tcatggtgtc atctaccggc tcgccgctac ccgcacaatg ggtaaaattg
    71641 acgccatagc ccagatgccc gagcagtgcc aattcaaagc ggcgcagcgc gggttctggc
    71701 gtaccagtgg cacctgcaag agactgaatg cagtgcaagt aatcgaaaaa gagttcagag
    71761 aagcgcgtct cgtattccag tacgcgggag agaagttcgt tgatgtacag accgctgtaa
    71821 agcgtgatac cgcttaatgg cagcgccagc gagacagctt cagcactgcg cagcgttttg
    71881 acttcgccac gcccgccgaa acgtagcaag agaggggtga aaggctgtaa tgcacctttc
    71941 agagtagagc gtttagagcg tgcgcctttg gcaaccagac gcacgcgacc cgattcctcc
    72001 gtgaagacgt ccagcatcag gctggtttcg ctccacgggc gactatgcag gacaaatgcg
    72061 cgctgccagc cttccatcgg agtgactctt aaagatcgtc aacgtaaccg agactgcgca
    72121 gtgcgcgttc gtcgtcggcc caaccggatt tcacttttac ccacagttca aggtggacag
    72181 gcgcttcgaa catttcctgc atgtctttac gcgcttcaat cccgatggtt ttgatcttgg
    72241 cccctttgtt gccaataacc atcttcttct gcccttcacg ctcaacgaga atcaaaccat
    72301 tgatgtcata accaccgcgt tcgttagaga cgaaacgttc gatctccacg gtcacggagt
    72361 acggcagttc agcgccgagg aaacgcatca gtttttcgcg gatgatttca gacgccataa
    72421 aacgctgtga gcgatcggtg atgtaatctt ccgggaagtg atgggtcgct tcagggaggt
    72481 gcttacgcac gattgctgcg atagtgtcga cattcagccc ggtttcagca gagatcggca
    72541 cgatatcgag gaagttcatc tgacttgcca ggaactgtaa gtgcggcagc agatcggctt
    72601 tttcctgcac gttatccact ttgttcaccg cgaggatcac cggcgctttg ccatcgcgca
    72661 gtttgttgag caccatttcg tcgtccggcg tccagcgggt gccttcaacg acaaaaatca
    72721 ccagctcaac gtcgccaata gagctgctcg ccgctttgtt catcaggcgg ttaatagcgc
    72781 gtttttcttc catatgcagg ccaggggtat caacgtagat cgcctgatac gcgccttcag
    72841 tatggatccc cacaatgcgg tgacgagttg tttgcgcttt gcgggaggtg atggagatct
    72901 tctgccccag cagtttgttc aacaatgtgg atttgccaac gttcggacgt ccgacgatgg
    72961 caataaatcc gcagtaactt ttatcgatgc tcattccagc tccagttttt tcaacgcctg
    73021 ttcggcggca gcctgctcag ccttacgacg gcttgaacct gtgccaacca ccggttcact
    73081 caggccgctg acctggcagt ggatagtaaa ttcctgatcg tgcgcttcgc cacgtacctg
    73141 gactaccaga taagtcggca gcggcagatg gcgaccctgc aaatattctt gcaagcgcgt
    73201 tttcggatct ttttgtttat cgcctgggct aatttcgtcc aaacgagttt gataccagtt
    73261 gaggattaat ttctcgacgg tttgaatatc actgtcgagg aatacgccac caattaatgc
    73321 ttcgacggtg tcggcgagaa ttgactcacg acgaaatcca ccgcttttaa gttcacctgg
    73381 ccctaaacgt aagcactcgc ctaactcaaa ttcgcgcgcc agttccgcca gcgtattgcc
    73441 acggaccagc gtggcgcgca tccggctcat atcgccttca tccacacgag ggaaacggtg
    73501 ataaagcgca ttggcgataa cgtagctcag aatagagtcg cctaaaaatt ctaaacgctc
    73561 gttatgttta ctgctggcac tacgatgagt taatgcctgc tgcaacagtt cctgatgatt
    73621 aaaagtgtag cccagcttcc gttgaagccg attaattacg atggggttca tgcgatacca
    73681 ataaataaat gcgtcaacaa ttcagcacac gaaacagacc tgatatacat ggctctgcta
    73741 actgcttcgc tgcagtttca gtttaccggt atatggggac caacgctgtt tcgtgtgccg
    73801 tggcaacctg gaggtgccaa ccttaaactt cgggggaata ttctatacac aacgacgggg
    73861 gatgtcgtta gccacgggag atttatctca taaataattc acgttgtcgc cataacggcg
    73921 acaacgtgaa cgaagatgag ctattaatgg atgccgccaa tgcgacttaa gcgcacacca
    73981 gtcggccatt cgccttcttg cttatcgaag ctcatccaga tagccgttgc ccgaccgacc
    74041 agattcgctt caggcacaaa cccccaataa cggctgtccg cgctgttgtc gcggttgtcg
    74101 cccatcatga agtattgtcc cggaggaaca atccaggttg ccagttgttg ccctggctgc
    74161 tggtaataca tccccacctg atcctgcgca atcggcactg tcagaattcg gtgcgtcaca
    74221 tcacccagtg tctctttacg ctcggaaaga cgaattccat tttctttggt ttcgtttttc
    74281 ggcacttcaa agaatccgct ggtcgcttcc ccaccattac ggcgtgagaa ggtctgaacg
    74341 aaatcgctcg gttccacgtt tgagtaggtg accggcagcg cgttttcaca cgcctggccg
    74401 gaactgcatc ccggttgaat cgtcagctct ttcgagaccg gatcataagt aactttatcg
    74461 cccggtaaac ccaccgcgcg cttgatgtaa tcaagctttg gatcttccgg atatttaaag
    74521 accacgatat ctccgcgttt cggatgaccg gtttcgatca gcgttttctg gtagatagga
    74581 tctttaatgc cataagcaaa cttttctacc agaataaaat caccgattaa cagagtcggc
    74641 atcatcgaac ctgacgggat ctggaacggt tcataaataa acgaacgcac aatcaatacg
    74701 atagccagta ccggaaaaac agaagcaccg gtttccagcc agccaggctt cggcgcaacc
    74761 tttttcaacg ttgctttatc cagtgagtca ccggcagccg cctgcgccgc tgcctgacgt
    74821 tcccgccgtt taggtgcgaa aaagaattta tccacgcacc ataaaatgcc cgtcaccagt
    74881 gtggcaatca ccagaatcag ggcaaacata ttcgccatgc caactcctaa gggttatttg
    74941 ttgtctttgc cgacgtgcag aatggcgagg aacgcttcct gcggcagctc gacgttaccg
    75001 atctgcttca tgcgtttctt accttctttc tgcttctgca gcagcttttt cttacggctg
    75061 atatcgccgc cataacattt agccagtacg tttttacgca gctgtttcac ggtggatcgc
    75121 gcaataatgt gcgtaccaat cgctgcctga atggcgatat caaactgctg gcgtgggatc
    75181 agatctttca tcttctccac caactcgcga ccgcggtttt gcgaattatc acggtgggtg
    75241 atcagcgcca acgcatcaac acgttcaccg ttgattaata cgtctacacg taccatgtcg
    75301 gacgcctgga agcgtttgaa gttgtaatcc agagacgcat aaccacgcga ggtagatttc
    75361 aggcgatcga agaagtcgag caccacttcc gccatcggga tctcgtacgt cagcgccacc
    75421 tgattaccgt ggtaaaccat attggtctgc acgccgcgtt tttctacgca cagcgtaata
    75481 acgttgccga gatatgcctg cggcagcagc atgtgacatt cggcaatcgg ctcgcgcagt
    75541 tcgtagatgt tatttaccgc tggcagcttg gatggactgt cgacgtaaat aacttctctt
    75601 gacgtggttt caacttcata cactacggtc ggcgcagtgg tgatcagatc cagatcgtat
    75661 tcacgttcca gacgttcctg gatgatctcc atgtgcagca ggccgaggaa gccgcagcgg
    75721 aaaccaaagc ccagcgcgct ggagctttcc ggctcataga acagtgaagc atcgttcagg
    75781 ctgagtttac ccagcgcgtc acggaaggct tcatagtcgt cggaacttac cgggaacaga
    75841 ccggcgtata cctgcggttt gactttctta aagccaggca gcgccttttc tgccggatta
    75901 cgcgccagcg ttaaggtatc gccgactgga gcgccgtgga tatctttaat cgcacatacc
    75961 agccagccta cttcgccaca tttcagttca gtgcggtcaa cctgtttcgg cgtgaagatg
    76021 cccagacggt cggcgttata ggtctgcccg gtactcatga ctttcacttt gtcgccctta
    76081 cgcagggtgc cgtttttaat acggataagt gaaacaacgc ccaggtagtt gtcgaaccat
    76141 gagtcgataa ttagtgcctg caacgggcct tccggatcgc cttccggcgg cggaatgtcg
    76201 cgcaccagac gttcgagaac gtcctgcacg ccaacgccgg ttttcgctga acagcgcacc
    76261 gcgtcggtgg cgtcgatgcc gacgatatct tcaatttctt ccgccacgcg ttcaggatcg
    76321 gctgccggca ggtcaatctt gttcagtacc ggcaccactt cgagatccat ttccatggcg
    76381 gtgtagcagt ttgccagggt ttgcgcttct acgccctgcc cggcgtcgac caccagcaat
    76441 gcaccttcac aggcagccag cgaacgggaa acttcatagg agaagtcaac gtggcccggg
    76501 gtgtcgataa agttcagctg ataggtttcg ccgtcagacg ctttgtagtc cagcgtcacg
    76561 ctttgcgctt tgatggttat gccacgctca cgctcaagat ccatggaatc gagaacctgc
    76621 gcctccattt cacggtcaga caggccaccg cagatctgga taatacggtc agacagcgtc
    76681 gatttaccgt ggtcaatgtg agctatgatc gaaaagttac gtatattctt cataaagtat
    76741 gattattgtg ccttaatgcc cggttaacca ggcttttaga agtcgctgtt ctgagcttaa
    76801 cgtctgtatt aatagaaacg ccgcattcta cactacaaca ttgaggcgag gaaatgttca
    76861 taccgtatgg attgtggtat ctggaaacgt cctcgcattt gttatgcaaa atgcaacaaa
    76921 gccagtgaaa tcactggctc gcgtcttccg aagatgtttc aaatcgcaca aggccaggcg
    76981 gcaatgccac gcttaagatg atcggttgcc attctgcccg ggcagcaaac ttacgcgagt
    77041 agccgcgcgc aatcaggaac ccgccaatac cgccgaggac cgcaccacat aatgctgcga
    77101 cgtcagaagc aaagagtagc tgaaatagcg aagcgatgag gaataatccc accagcggcg
    77161 acatataaac cagtaatgcg gagctaagca ggctgccttc ggcgatcccc aattccactt
    77221 tttgccccgg cactaacggc tcatcacagg gtacgacaat ggtatgcgtg gtttgcgggc
    77281 caagtttatt taacacgcgg ctaccgcaac cggcgcgtga agcgcagctg ctgcatgaag
    77341 ctttaacatc acaactgacc agcgcctgcc cgttttgcca ggagacgacg gtagcccact
    77401 ctttgatcat tgcgctgccc cgaacttaat attctcggca atgcgtttcg ccgtttgcgg
    77461 cggcagttca ccgacaatgg tgatttcggc gttatcacgt acgcttgtac tgacggttct
    77521 gcgtccggtg cgcaacatct gatcggtgct cgatggcgta gcgcggttaa cgtttaccga
    77581 gaagctgaat aatccgtcgg aatagagacg tgattcgata ggcatgttgt ccatcgtcgg
    77641 tagcggacgt cgactactgg aaacttcgct aaaaccctgt ggcaaccagg ttggcgtcca
    77701 gctgaattta gctttttcac ctacaggaac agaaagcaac ggcggcaaat ttgccttcgc
    77761 cagcgtctgc atactgctgc tgatatcctg attgacgtta aaagcaatca cgcgaaattg
    77821 ttccagcgtt tcaccatcgc gatcaaggag atcaacccgc atcggtaatt tcgattcggt
    77881 gtccatccac acgatgtagc tgtagcgtgt accatctcgg gcaaccacgc gaatgacttc
    77941 gcaaagacga tcagcaatac gcgtgcgccc gacggagata aagtcgtagt aaggagaaag
    78001 gcgtttgaaa tcggtataga taagcgatgg cagagaatca acaatgtaat cgccattaag
    78061 cgtgaacggt tcaagtcccg gttcaaaata gctgatttca ttgccgcgct gtaccacttc
    78121 ccggcgcggg ccatccattt gcaacaattg tgcaagagga cggttatcga ggcgtgcatg
    78181 tcgataacgc agagactcaa caccctgttt attgatgctg atgaatgaca gctcgtaatt
    78241 cagtgactga ctggccaggt tcatctgctg taataacgcc ccggacgcgg gagtggccga
    78301 ggcgttagca gagaataaca ggctacctgt cactaatgac atggcaaacc aaagttgctt
    78361 cattactgcg attgcgttcc taaagtttga attcctggca cctgtacagc ggcttgctgg
    78421 gtttgcgcct gctcaaactg aagctgttca gagtggagtc ggcgttgcag ttcgtaatcc
    78481 tgcaacattg cattaatgcg acgacgctgc tcctgtacct gctgctgttg accattgttt
    78541 gcggtcgctt cagaaggtac tcccaggctt accgggctgg ctttacccat catcggcagt
    78601 gtattaaata ccggcgtttc gggctgctgg gacgtttcag attgtccatt atagtgctgg
    78661 acgccaacga taactgcaag cgatacgcat gctgctacgc ccatttgggt aagctgtgcc
    78721 gcccacggac gtactttctg ccagaatggc attttctgcc attgatgcgg cgcaggctgg
    78781 gcttccggga tcaatgtcgc cggttgacgt actggctctt cttcaatggc ggccatcacg
    78841 cgtgaagaga tatcgaaatg gagcacctcg ggagtatcac cccgcattga gtcacggatt
    78901 aagtgatagc tttcccaggt tttctgcatt tctgggttat gagccagttc gttaagcagc
    78961 tcactatcca gcgtttcgcc atccattaaa gcggaaagtt gttctttctg catgcctaat
    79021 acccttatcc agtatcccgc tatcgtcaac gcctgataag cggttgaact ttgttatcaa
    79081 tagcttccct cgctcggaag atacgtgaac gcaccgtacc taccggacaa tccatgatag
    79141 cggctatctc ttcatagctc aggccatcca gctcccgcaa ggttattgcc atgcgcaaat
    79201 cttccgggag ggactcaata gttcggaaaa ctatctgtct cagttcttct gacaacatta
    79261 agttctcagg gttcgaaatt tctttcaacg cgccgccact ttcgaagttt tcagcttcaa
    79321 tggcatccac atcactggaa ggtggacgac gcccctgagc aaccaggtaa tttttcgctg
    79381 tatttacagc aatccgatac agccatgtat aaaaagcgct atctccccgg aacgaatcca
    79441 gcgcacgata ggctttaata aaagcttctt gtaccacatc gggaacatca cccgacggca
    79501 catagcggga aaccagactc gccactttat gctgatagcg cactaccagt aggttaaagg
    79561 ctttctgatc tcccttctgg acccgttcaa ccaggacctg gtccgttaac tgctcgctca
    79621 tccgaggtaa agtctcccca aaccaaattt ccacgcgcta tcgaaacgcc actccattag
    79681 ctgcaatttg agcaagcaaa gggttagagt gtctcgtttt tgtaaagttc cgtaacgcat
    79741 ctgtttttgt ttgtcatgct gtagacggat cattatctat cattataagt ctacagaatc
    79801 tgaacatcgc attatctgtg tagaaatgcc catttaactg cctgaagagt aacccaacgg
    79861 cctttttatt tcaccaccta atcctccacc agccagtaac ttctcttttt ctcgccgccc
    79921 tgcgtcagcg tgtttagcca ctgtaacaaa tattaaaata gcaggtgttt attcgcacaa
    79981 catgatgcta tgctgaccaa acagtgttta gtaaattaaa caaagaaaat gaatactctc
    80041 ccagaacatt catgtgacgt gttgattatc ggtagcggcg cagccggact ttcactggcg
    80101 ctacgcctgg ctgaccagca tcaggtcatc gttctaagta aaggcccggt aacggaaggt
    80161 tcaacatttt atgcccaggg cggtattgcc gccgtgtttg atgaaactga cagcattgac
    80221 tcgcatgtgg aagacacatt gattgccggg gctggtattt gcgatcgcca tgcagttgaa
    80281 tttgtcgcca gcaatgcacg atcctgtgtg caatggttaa ttgaccaagg ggttttgttt
    80341 gatacccacg ttcaaccgaa tggcgaagaa agctaccatc tgacccgtga aggtggacat
    80401 agtcaccgtc gtattattca tgccgccgac gccaccggta gagaagtaca atccacgctg
    80461 gtgagcaagg cgcagaacca tccgaatatt cgtgtgctgg agcgcagcaa cgcagttgat
    80521 ctgattgttt ctgacaaaat tggcctcccg ggcacgcgac gggttgttgg cgcgtgggta
    80581 tggaaccgaa ataaagaaac ggtggaaacc tgccacgcaa aagcagtggt actggcaacc
    80641 ggcggtgcgt cgaaggttta tcagtacacc accaatccgg atatttcttc tggcgatggc
    80701 attgctatgg cgtggcgcgc aggctgtcgg gttgccaatc tcgaatttaa tcagttccac
    80761 cctaccgcac tgtatcaccc acaggcacgc aatttcctgt taacggaagc actgcgcggc
    80821 gaaggcgctt atctcaagcg cccggatggc acgcgtttta tgcccgattt tgatgagcgc
    80881 ggcgaactgg ccccgcgcga tattgtcgcc cgcgccattg accatgaaat gaaacgcctc
    80941 ggcgcagatt gtatgttcct cgacatcagc cataagcccg ccgattttat tcgccagcat
    81001 ttcccgatga tttatgaaaa attgctcggg ctggggattg atctcacaca agaaccggta
    81061 ccgattgtgc ctgctgcaca ttatacctgc ggtggtgtaa tggttgatga tcatgggcgt
    81121 acggacgtcg agggcttgta tgccattggc gaggtgagtt ataccggctt acacggcgct
    81181 aaccgcatgg cctcgaattc attgctggag tgtctggtct atggctggtc ggcggcagaa
    81241 gatatcacca gacgtatgcc tgatgcccac ggcgtcagta cgttaccgcc gtgggatgaa
    81301 agccgcgttg agaaccctga cgaacgggta gtaattcagc ataactggca cgagctacgg
    81361 ctgtttatgt gggattacgt tggcattgtg cgcacaacga agcgcctgga acgcgccctg
    81421 cggcggataa ccatgctcca acaagaaata gacgaatatt acgcccattt ccgcgtctca
    81481 aataatttgc tggagctgcg taatctggta caggttgccg agttgattgt tcgctgcgcg
    81541 atgatgcgca aagaaagccg ggggctgcat tttacgctgg attacccgga actgctcacg
    81601 cattccggtc catcgatcct ttcccccggc aatcattaca taaacagata aaaagcctgg
    81661 gtcagcgccg tatacgcttc ggaatagttc tggtctggcc cacgaatgac taagcgatcg
    81721 ctaaagcatt ctcccgcctg cggggagaat gccagcagca cccgatgcgg cagtcgcgct
    81781 tcgttttccg ccacatccgt ccgcaaacgt aaatgccagc ccatgcttaa tgccagttca
    81841 gtaaaaccat taccaatctg ctctggcagc accacgcaga aaaatccctc ttcggtaatg
    81901 cactccgccg cacaggtcag cagcgatggg tgatcaagcg tagtggtata gcgagcctgt
    81961 tcccgttgag gtgtcgcgca ctctactccc tgctgatagt aaggtgggtt actgatgatt
    82021 aaatcgaagc gtactgtctg ctgtgtgatc cactgctgaa tatccgccgt atggacgtta
    82081 atccgctctg cccacgggga ctggttgata ttttcctgcg cctgcgcggc agcttcactt
    82141 tccagctcaa cggcatcaat tatcacgctg tcactggttc gctgcgccag catcagtgcc
    82201 agcaacccgc tacccgcgcc gatatcaagg caacgtttca ccccagccac cggtgcccat
    82261 gcgcccagca aaatgccatc cgttcccact ttcatcgcac agcgatcgtg agcaacaaaa
    82321 aactgtttaa aagtaaatcc attacgacga agcacggatg tagactgtga catgaaaata
    82381 aaaccttgca ggaaaaacgg cgatagcacc gggtgagaac aatacccgag aagcgatatc
    82441 catacaaaca gatgaagatt gcagccgtaa cgtctataat cagcgcccca cacagaggta
    82501 gaacatgact gtaacgactt tttccgaact tgaactcgac gaaagcctgc tggaagccct
    82561 ccaggataaa ggtttcactc gcccgaccgc cattcaggct gccgccattc cgcctgcgct
    82621 cgatggccgt gatgtactcg gttctgcgcc gacaggcacc ggtaaaacgg cggcgtatct
    82681 gctgccagcg ttgcagcacc tgctcgattt cccgcgtaag aaatccggtc cgccgcgtat
    82741 tttgatcctc accccaactc gcgagctggc gatgcaggtg gccgatcatg cccgcgaact
    82801 ggcgaaacat acgcatctgg atatcgccac catcaccggc ggcgtagcct atatgaacca
    82861 cgcggaagtg ttcagcgaaa atcaggacat cgtggtcgcc acgaccggac gtctgctgca
    82921 atacataaaa gaagagaact tcgattgccg cgcggttgaa acgctgatcc tcgacgaagc
    82981 agaccgtatg ctggatatgg gcttcgctca ggatatcgaa catattgctg gcgaaacgcg
    83041 ctggcgtaaa cagaccctgc tcttttcggc aacgctggaa ggcgatgcga ttcaggactt
    83101 tgccgagcgt ctgctggaag atccggtgga ggtttctgcc aatccgtcca cccgtgagcg
    83161 caaaaaaatt catcagtggt attaccgcgc cgatgacctg gaacataaaa ccgcattgct
    83221 ggtgcatctg ttaaaacagc cggaagcgac ccgctcaatt gtgtttgtgc gtaagcgtga
    83281 acgtgtgcat gagctggcaa actggctgcg cgaagcgggc atcaacaact gctatctcga
    83341 aggtgagatg gtacaaggca agcgtaacga agcgatcaag cgtttgaccg aaggtcgcgt
    83401 aaacgtactg gttgcaaccg atgttgccgc gcgcggtatc gacattcctg acgtcagcca
    83461 cgtctttaac ttcgatatgc cgcgcagtgg cgatacttat ttgcaccgta tcggacgtac
    83521 cgcgcgcgcc ggtcgtaaag gcaccgcaat ttcgctggtg gaagcccatg accatttgct
    83581 gctgggcaag gttggccgtt acattgaaga gccgattaaa gctcgcgtta ttgatgagtt
    83641 acgcccgaaa acgcgtgcgc caagcgagaa gcagaccggc aagccatcga agaaagtact
    83701 ggcaaaacgc gccgagaaga aaaaagctaa agagaaagag aaaccgcgtg tgaaaaaacg
    83761 ccatcgcgac accaaaaata ttggtaagcg ccgtaaacca agcggaacgg gcgtgccacc
    83821 gcaaacgaca gaagagtaat ctcaatgccg ggtttaagcc tggtattaaa agtgctggca
    83881 aacgcaaaac tgcctgatgc gctacgctta tcaggcctac gtggctcatg caatatattg
    83941 aatttgcacg atcttgtagg ccggataagg cgttcacgcc gcatccggca tgaataaagc
    84001 gcactttgtc gacaagttaa atgccgggtt taatcccgac attattttta tccagccaca
    84061 aaactctctt caacacactg aataaaagtg tgcatcgccg ggctaaccgc ttttccagca
    84121 tggtgtgcgc acatggccgt aatggtctgc gactgttcgc caaaaggcaa ttcaattaat
    84181 tctccgctct caagctcttt ttccaccgca aagcggggca agtaactgac accgatattc
    84241 gccgcgacac aacgtttgat actttcaatg cttaacagct caatggtgtt ttccaccgtg
    84301 atccgccgct gacgcagcgt gctctcaaat atctgccgga agacacattg cggttcgttg
    84361 ataataaagc tacaggcgtt atgtcttccc ggctcagtaa aatcgacatc tgcaatttgc
    84421 ggtgaagcca ccagcaccag cgattgttca cccaactctc gtcggttcag ggcatcatca
    84481 ttccctacgc gataaaagac gccgacatcg gcctcatcat tcagtagcgc atcacggatc
    84541 acgtaacagt tcagcgactg caacgataaa cgcacttttg gtgcgcgttg ccgaaaacgc
    84601 tgcaacacct gcggcattcg gtaagagagc aacgtttcgc ccgaaacaac gcgtagttcc
    84661 ccgtccggat ctgactcctt tttggcagcc tcacgaagcg tatccatcac tcgggtaagt
    84721 tcataaatgt gcggcagcaa cttttttcct tcgcgggtaa ggcacatccg tcggccaatt
    84781 ttctcaaata actggactga aaactcctgc tcaagctgct gaatatgaaa agtcaccgtc
    84841 gattgtgtac agcacaattt ttgcgaagct cgcaaaaagg aaccctcttc caccacggtt
    84901 ttaagcgtaa taaaacggcg cagatccata accccaaacc tatcgaaaat atcgaatcta
    84961 gaatataaaa acattcattt ttttaaatgt tccgtgtcgg gtactgtcta ccaaaacaga
    85021 ggagataaca agtgacaccg ccccttttaa gtgctttttg gacttacacc ctgattaccg
    85081 ctatgacgcc aggaccgaac aatattctcg cccttagctc tgctacgtcg catggatttc
    85141 gtcaaagtac ccgcgtgctg gcagggatga ctctgggatt tttgattgtg atgttactgt
    85201 gtgcgggcat ttcattttca ctggcagtga ttgacccggc agcggtacac cttttgagtt
    85261 gggcgggggc ggcatatatt gtctggctgg cgtggaaaat cgccaccagc ccaacaaagg
    85321 aagacggact tcaggcaaaa ccaatcagct tttgggccag ctttgctttg cagtttgtga
    85381 acgtcaaaat cattttgtac ggtgttacgg cactgtcgac gtttgttctg ccgcaaacac
    85441 aggcgctaag ctgggtagtt ggcgtcagcg ttttgctggc gatgattggg acgtttggca
    85501 atgtgtgctg ggcgctggcg gggcatctgt ttcagcgatt gtttcgccag tatggtcgcc
    85561 agttaaatat cgtgcttgcc ctgttgctga tctattgcgc ggtacgcatt ttctattaac
    85621 gaaaaaaagc ggaagaggtc gccctcttcc gcttagtaac ttgctactta agccttacag
    85681 gctttcagta aaggtacgag cgataacgtc gcgctgctgt tccggagtca gagagttaaa
    85741 gcgaactgca taaccggata cacggatggt cagctgcgga tatttttccg gatgcttaac
    85801 tgcatcttcc agagtttcac gacgcagaac gttaacgttc aggtgttgac caccttcaac
    85861 gcgaacttct ggtttcactt ctactggaac ttcacggtat tcaatgtcac ccagtttgct
    85921 tactgcaacc acttcatctt ctgcataacc tgcttttgca acgatgcaac gtgcttcgcc
    85981 tttttcgctg tccagcagcc agaaagagtt cagcagatcg tcgttagcgg ctttagtaat
    86041 ctggatacct gtaatcatgt gatgcctccc cggcaaaatt atttgatttg ttcagcctgt
    86101 cgcggccaat tggtaaaacc attgttgctt gagtgtatat atactcctca accacccttg
    86161 attctttgat ttaaatcaat aaaaaccaca catcgattgt ggtcgtaaat ggattttatt
    86221 gttttacatc aacttatgcg ggtgtgaaat tttaccaatt tacatttttt tgcactcgtt
    86281 taagtctaaa aaatgagcat gattttgttc tgtagaaaga agcagttaag ctaggcggat
    86341 tgaagattcg caggagagcg agatggctaa cgaattaacc tggcatgacg tgctggctga
    86401 agagaagcaa caaccttatt ttcttaatac ccttcagacc gtcgccagcg agcggcagtc
    86461 cggcgtcact atctatccac cacaaaaaga tgtctttaac gcgttccgct ttaccgagtt
    86521 gggtgacgtt aaagtggtga ttctcggcca ggatccttat cacggaccgg gacaggcgca
    86581 tggtctggca ttttccgttc gtcccggcat tgccattcct ccgtcattat tgaatatgta
    86641 taaagagctg gaaaatacta ttccgggctt cacccgccct aatcatggct atctcgaaag
    86701 ctgggcgcgt cagggcgttc tgctgctcaa tactgtgttg acggttcgcg caggtcaggc
    86761 gcattcccac gccagcctcg gttgggagac gtttactgat aaagtcatca gcctgattaa
    86821 ccagcaccgc gaaggcgtgg tgtttttgtt gtggggatca catgcgcaaa agaaaggggc
    86881 gattatagat aagcaacgcc atcatgtact gaaagcaccg catccgtcgc cgctttcggc
    86941 gcatcgtgga ttctttggct gcaaccattt tgtgctggca aatcagtggc tggaacaacg
    87001 tggcgagacg ccgattgact ggatgccagt attaccggca gagagtgagt aaatttgcgg
    87061 taaaatgccg gatggcagag ttgccacccg gctgatttat caggctttat tctgacgcca
    87121 ccattcacca agcaaaacgc cggttgcaac agagatattc agcccggcaa cgttgcccgt
    87181 accgtcaatc ttcacgcgca gatcgttcgg atcgcgtgcg gcatccggta acccttcata
    87241 ttcctgaccc agcaccagta ccattttcgc tggcagactg gttttgaaca gcggtttccc
    87301 ctgctcgctg gaagtggtca ctacggtgta acctgcctga cggaaatcat ccaacacgtt
    87361 aacaatgttg tcgccagtaa tcggctgaac gtgctctgcg ccgccttctg cggtacggat
    87421 agccgccccc gactccagca gtgccgcatc ctgcaccaca acgcctttca cgccgaagtg
    87481 cgcgcagctg cgcatcatgc cacccaggtt atgcgggtta gattcgtttt ccagcgccag
    87541 tacacaatcc tgcgcgcctg cctggcttac ccactgctgc acggttgtgc cattacgctt
    87601 tttgatcagg aagcaaacgc cgccgtgatg ttccgtgcct gacgcttttg tcaattccgc
    87661 ttcatccacc acatggtacg ctttgcggtt tgctgccatc cagcgcaagg cttctttaaa
    87721 acgcggcgtt acactctgga taaaccaggc gcgaacaatc gcttccggac ggctctggaa
    87781 aagtgcctga catgcatttt cgccgtagac gcgggtttct tccgcacgct gacgacgcaa
    87841 cacttccgga tcaataaaac ttttaccact gatgccaccg tgatcggcct tttccggcgt
    87901 ctcatcaccc ggcgcgcggg aaaccgtgcg ccacggcgaa tcttcccact tgcggtcacg
    87961 gggctgactc tgtttgtcat cgcgggcggg gcgacggcca ccgtcagcac gagattttcc
    88021 tggacgcccg ccccctttcc cggtacgcgg gttgtgggta cgtttatcag aatcatcatc
    88081 actgcggaca tacatcactt tgaccttgcc gcttttacct ttcatttcat cgttcatgct
    88141 tttctccacc agcgctgcgc gaagcgcgca gattacccga agtccgcgcg gttcgccatg
    88201 atttcgtacc aaagcctgcg actatcatac ctattgaata aaacagattg ttgtctggaa
    88261 caatgtcccc gataatatgt aacatattag aaacataccg gcgtcgttgc cgataagtct
    88321 ccttactcat cccgaggtta gttatgaata ccgtttgtac ccattgtcag gccatcaatc
    88381 gcattcccga cgatcggatc gaagatgcgg caaaatgcgg acgctgcggt cacgacttgt
    88441 ttgacggaga ggtgattaat gcgaccggtg aaacactcga caaattgctg aaggatgatc
    88501 tacctgtggt gatcgacttc tgggcaccgt ggtgcggccc ctgccgtaat ttcgcaccaa
    88561 tttttgaaga tgtcgcgcaa gagcgtagcg gtaaagtgcg ctttgtgaaa gtgaataccg
    88621 aagctgaacg tgaattgagc agtcgctttg gaattcgtag tataccgacg atcatgattt
    88681 tcaaaaaccg tcaggttgtc gacatgctta atggcgcagt accgaaagcg ccgttcgata
    88741 gctggctgaa cgaatctctt taatcttacc ggggcgcatc ttgtgccccg ttttctcctc
    88801 tgcgacaatg gcgttttttc gacgctctct tatgaccgaa aacgctgttc tccagttacg
    88861 cgccgagcgt attgcgcgcg caacacgtcc ttttcttgcc cgcggtaatc gcgttcgtcg
    88921 ctgccaacgc tgtcttttgc cagagaaatt atgtctctgt tcgacaatta ccccagcaca
    88981 agcaaaaagt cgcttctgtt tgctgatgtt cgacaccgag ccaatgaagc ccagtaatac
    89041 cgggcgtctg attgctgata ttttgcctga taccgttgcg tttcaatggt cgcgtaccga
    89101 accctcgcag gatttgctgg atctggtaca aaacccggat tatcagccaa tggtggtctt
    89161 tcccgcctcg tatgctgatg agcaacggga agtgatcttc acaccacctg ccggtaagcc
    89221 accgctgttt atcatgctcg atggtacctg gccggaagct cgcaagatgt ttcgtaaaag
    89281 tccgtatctg gataatcttc ccgtcatttc cgtcgatctt tcccggcttt ctgcctatcg
    89341 cctgcgtgaa gcccaggctg caggccaata ttgtactgcc gaggtagcca tcgcactgtt
    89401 agatatggcc ggcgataccg gggcggcggc agggttaggc gagcatttta cccgctttaa
    89461 aacacgctat ctggcaggaa aaacgcaaca tctgggtagc atcacagcag aacaattaga
    89521 aagcgtttaa aatcattcgg tcacttctgc gggagaccgg tatgagtcag cgaggactgg
    89581 aagcactact gcgaccaaaa tcgatagcgg taattggcgc gtcgatgaaa cccaatcgcg
    89641 caggttacct gatgatgcgt aacctgctgg cgggaggctt taacggaccg gtactcccgg
    89701 tgacgccagc ctggaaagcg gtgttgggtg tgttggcctg gccggatatt gccagcttgc
    89761 cctttacacc cgaccttgcg gttttatgta ccaatgccag ccgtaatctt gctcttctgg
    89821 aagagctcgg cgagaaaggc tgtaaaacct gcattattct ttccgccccg gcatcgcaac
    89881 acgaagatct ccgcgcctgc gccctgcgcc ataacatgcg cctgcttgga ccaaacagtc
    89941 tgggtttact ggctccctgg caaggtctga atgccagctt ttcgcctgtg ccgattaaac
    90001 gcggcaagct ggcgtttatt tcacaatcgg ctgccgtctc caacaccatc ctcgactggg
    90061 cgcaacagcg tgagatgggc ttttcctact ttattgcgct cggcgacagc ctggatatcg
    90121 acgttgatga attgctcgac tatcttgcgc gcgacagtaa aaccagcgcc atcctgctct
    90181 atctcgaaca gttaagcgac gcgcgacgct ttgtttcggc ggcccgtagt gcctcgcgta
    90241 ataaaccgat tctggtgatt aaaagcggac gtagcccggc ggctcaacgt ctgttgaaca
    90301 ccacggcagg aatggatccg gcgtgggatg cggctattca gcgtgccggt ttgttgcggg
    90361 tacaggacac ccacgagctg ttttcggcgg tggaaaccct tagccatatg cgcccgctgc
    90421 gtggcgaccg gttgatgatt atcagcaacg gtgctgcgcc tgccgcgctg gcgctggatg
    90481 ccttatggtc acacaatggc aagctggcaa cgctaagcga agaaacctgc cagaaactgc
    90541 gcgatgcact gcctggacat gtggcaatct ctaacccgct cgatctgcgc gatgacgcca
    90601 gcagcgagca ttatgtcaaa acgctggaca ttctgctcca cagccaggat tttgatgcgc
    90661 tgatggttat tcattcaccc agcgcagcgg ctcccgcaac agaaagcgcg caagaattaa
    90721 ttgaagcggt aaagcatcat ccccgcagca aatatgtctc tttgctgacg aactggtgcg
    90781 gcgagcactc ctcgcaagag gcacgacgtt tattcagcga agccgggctg ccgacctacc
    90841 gtaccccgga aggaaccatc actgctttta tgcatatggt ggagtaccgg cgtaatcaga
    90901 agcaactacg cgaaacgccg gcgttgccca gcaatctgac ctccaatacc gcagaagcgc
    90961 atcttctgtt gcaacaggcg attgccgaag gagcaacgtc actcgatacc catgaagttc
    91021 agcctatctt gcaagcatat ggcatgaaca cgctccccac ctggattgcc agcgatagca
    91081 ccgaagcggt gcatattgcc gaacagattg gttatccggt ggcgctgaaa ttgcgttcgc
    91141 cggatattcc acataaatcg gaagttcagg gcgtcatgct ctacctgcgt acagccaatg
    91201 aagtccagca agcggcaaac gccattttcg atcgcgtaaa aatggcctgg ccgcaggcac
    91261 gggtccacgg cctgttggtg caaagtatgg ctaaccgtgc tggtgctcag gagttgcggg
    91321 ttgtggttga gcacgatccg gttttcgggc cgttgatcat gctgggcgaa ggcggtgtgg
    91381 agtggcgtcc tgaagatcaa gccgtcgtcg cgtttccgcc gctgaacatg aacctggccc
    91441 gctatctggt tattcagggg atcaaaagta aaaagattcg tgcacgcagt gcgctacgcc
    91501 cattggatgt tgcaggcttg agccagcttc tggtgcaggt ttccaacttg attgtcgatt
    91561 gcccggaaat tcagcgtctg gatattcatc ctttgctggc ttctggcagt gaatttaccg
    91621 cgctggatgt cacgctggat atctcgccgt ttgaaggcga taacgagagt cggctggcag
    91681 tgcgccctta tccgcatcag ctggaagaat gggtagaatt gaaaaacggt gaacgctgct
    91741 tgttccgccc gattttgcca gaagatgagc cacaacttca gcaattcatt tcgcgagtca
    91801 ccaaagaaga tctttattac cgctacttta gcgagatcaa cgaatttacc catgaagatt
    91861 tagccaacat gacacagatc gactacgatc gggaaatggc gtttgtagcg gtacgacgta
    91921 ttgatcaaac ggaagagatc ctcggcgtca cgcgtgcgat ttccgatcct gataacatcg
    91981 atgccgaatt tgctgtgctg gttcgctcgg atctcaaagg gttaggctta ggtcgacgct
    92041 taatggaaaa gttgattacc tatacgcgag atcacggact acaacgtctg aatggtatta
    92101 cgatgccaaa caatcgtggc atggtggcgc tagcccgcaa gctcgggttt aacgttgata
    92161 tccagctcga agaggggatc gttgggctta cgctaaatct tgcccaacgc gaggaatcat
    92221 gagtaaggta ctggaaatgt tgaccactta atcgggactg gtgttattat tgcccgctta
    92281 tgtcgtctgc attgcacaga ggacccttca atgaacagag aagaaatgca ctgtgatgtt
    92341 gtcaaaattt aagcgtaata aacatcaaca acaccttgcc caactaccca agatttctca
    92401 atcagttgat gatgtcgatt tcttttacgc tcccgccgac ttccgggaga cgctgctgga
    92461 aaaaatagcc agcgcgaagc agcgcatttg cattgtcgcc ctgtatctcg aacaggatga
    92521 cggtggcaaa ggcattctga acgcgttgta tgaagctaaa aggcagcgtc cggaactgga
    92581 tgtgcgggtg ctggtcgact ggcatcgtgc acaacgtgga cgcattggcg ctgcggcatc
    92641 taacactaac gctgactggt actgccgcat ggcgcaggaa aatccgggcg tagatgttcc
    92701 ggtttatggc gttccaatca atacccgtga agcccttggt gttctgcact ttaaaggctt
    92761 tatcatcgac gatagcgtac tttatagcgg tgccagcctg aacgatgttt acctgcatca
    92821 gcacgataaa tatcgctacg accgttatca tcttatccgt aaccgtaaga tgtcagacat
    92881 tatgtttgaa tgggttacac agaatattat gaatggccgc ggcgttaatc gtctggatga
    92941 tgttaatcgg ccaaaaagcc cggaaatcaa gaacgatatt cgtctgttcc gccaggagct
    93001 gcgtgatgcc gcttatcatt tccagggcga tgccgacaac gatcagcttt ccgtaacgcc
    93061 gctagtgggg ctggggaaat cgagtctgtt gaacaagacc attttccatc ttatgccttg
    93121 tgccgagcag aaactaacca tctgtacgcc atacttcaac ctgccagcaa tccttgtgcg
    93181 caatattatc cagttgctgc gcgaagggaa aaaggtcgaa attattgttg gtgataaaac
    93241 cgcgaacgac ttctacattc cggaagatga acctttcaag ataattggcg cattgcctta
    93301 tctctatgag atcaatctgc gtcgtttcct gagccgtttg cagtattacg tcaatactga
    93361 ccagctagtg gttcggttat ggaaagatga cgacaacacc tatcacctaa aagggatgtg
    93421 ggttgatgat aagtggatgt tgatcaccgg taataacctg aacccgcgcg cctggcgtct
    93481 ggatctggaa aacgccattt tgatccacga tccacaactg gagcttgcgc cgcagcgaga
    93541 gaaagaactg gagctgatcc gcgagcatac caccatcgtt aagcactatc gcgatctgca
    93601 aagtattgcc gattatccgg tgaaggttcg taaactcatc cgccgtttgc gccgtatccg
    93661 catcgaccga ttaattagcc gcatcctgta atcacaaccc cgtcctgtac ggggtttgtt
    93721 ttttggaggc cacgttttgc gtattctttt tgtctgttcg ctattgttac tttctggatg
    93781 cagccatatg gctaacgata gctggagcgg gcaggataaa gctcaacact ttatcgcctc
    93841 ggcgatgctt tccgccgccg gaaatgaata ttcacagcat caggggatga gccgggatcg
    93901 cagtgccatg tttggattga tgttctctgt cagtttgggg gcgtcaaaag agctttggga
    93961 tagccgcccc gaagggagcg gctggagctg gaaggatttg gcctgggatg tcgccggtgc
    94021 aagcaccggc tataccgtct ggcaactgac ccgtcactaa agacgcatcc ccttcccttt
    94081 gcgatgtagc atcaaagaaa ccagaaacgc caccacggcc atcaaggtca cataccagaa
    94141 gaaggctgtt tccattccta ttgatttcag cgacaacgct acgtactccg ccgaaccacc
    94201 aaatatagca ttagcgaccg catatgacag gccaacgcct aatgcgcgaa cctgtgccgg
    94261 gaacatctca gccttcagta ttccactgat tgatgtataa aaactcacta tcagcagggc
    94321 acacatcacc agaccaaaag cggcataagg cgaggaaacg ttttgcaatg ctgagagaat
    94381 aggaacggta aaaatggctg ccagcgaacc gaaacataac attgaggtac ggcgaccaat
    94441 cttatccgac agcgcgccaa tgagtggttg aataagcatg aatacaaaca atgcggcagt
    94501 cataatgcca ctcgccacgt tggcatgcat tcccgcagta tttaccagat acttctgcat
    94561 ataagtggtg aaggtataga aacaaaggga gcccgcagcg gtaaaaccga gaaccatgat
    94621 gaatgcacgg cgattgcgcc ataatccttt cagagatcca gcttctttta aagcgcgcgt
    94681 ttcttgttgc gaagtttcat ctaattgacg acgtaaccac aacgccacaa cagctaacac
    94741 agctcctaac gcgaaaggaa tacgccatcc ccactctctg agtgcagcgt cttccatggt
    94801 gtgttgtaaa accacgacaa ccagtagggc tagcagttgt ccgccgatca acgtcacata
    94861 ctgaaatgat gcgtaaaaac ctttgcgccc ttcaacggca acttcactca tataggtggc
    94921 gctggtgcca tattctccgc caacggataa tccctgaaat aaacgagcga gaagcaataa
    94981 tgccggagcc cacgtaccta tagtttcata acctgggagg caggcgataa ccagcgatcc
    95041 gaaacacatc atacacaccg ataacagcat cgattttttg cgaccatgtt tatcggctat
    95101 gcggccaaat agccaaccgc ctattgggcg catcaggaat cccgcagcaa aaacacctgc
    95161 tgtttgtagt agttgagtcg tcgtgttccc ggaagggaag aaaatgtggg cgaagtagag
    95221 tgaacagaac gagtagacat agaaatcgaa ccactcgacc agattacctg aagaggcccc
    95281 cacaatcgcc caaatgcggc gacgtgtatc actacttgtc agtttgctgt ctgccgttac
    95341 agtactttca gccatgccat tatgtctcct gccgtaatcc gatgcttttg tcggtcgctt
    95401 ttgtttattt cttttggtaa agaaaatgtt atacatttgt tgcgtatcat tatgcaacct
    95461 taaccatgaa tttagttagc aggaaaatgg ttattgagga gcttaaggat aaatttctgg
    95521 tgaggagaac acgtatggag gtgggcaagt tggggaagcc gtattcgctg ctgaatctgg
    95581 catatgtggg agtataagac gcgcagcgtc gcatc
//
LOCUS       DAZFQD010000003        78415 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.003, whole genome shotgun sequence.
ACCESSION   DAZFQD010000003 DAZFQD010000000
VERSION     DAZFQD010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 78415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 78415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..78415
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.003"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            24..341
                     /locus_tag="ACHKTW_000192"
     CDS             24..341
                     /locus_tag="ACHKTW_000192"
                     /EC_number="5.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HFO6461288.1"
                     /translation="MQPVFLFKKRTGAMGGHVAGTAGFCVAVPAFYSWSMSEVIDKDT
                     RSSERTDYPHYASEVALAFGSRVVGLELAKMIVDAWLGAQYEGGRHQQRVEAITAIEQ
                     RRN"
     gene            410..739
                     /gene="yjdP"
                     /locus_tag="ACHKTW_000193"
     CDS             410..739
                     /gene="yjdP"
                     /locus_tag="ACHKTW_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDRRRQL repeat protein YjdP"
                     /protein_id="HFO6461289.1"
                     /translation="MKRFPLFLLFTLLTLSTVPAQADIIDDTIGNIQQAINDAYNPDR
                     GRDYEDSRDDGWQREVSDDRRRQYDDRRRQFEDRRRQLDDRQRQLDQERRQLEDEERR
                     MEDEYGR"
     gene            complement(886..1644)
                     /gene="phnP"
                     /locus_tag="ACHKTW_000194"
     CDS             complement(886..1644)
                     /gene="phnP"
                     /locus_tag="ACHKTW_000194"
                     /EC_number="3.1.4.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism protein PhnP"
                     /protein_id="HFO6461290.1"
                     /translation="MSLTLTLTGTGGAQGVPAWGCECAACARARRSPQYRRQPCSGVV
                     KFNDAITLIDAGRHDLTDRWSPGSFQQFLLTHYHMDHVQGLFPLRWGVGDVIPVYGPP
                     DEQGCDDLFKHPGLLDFSHTVEPFVVFDLQGLQVTPLPLNHSKLTFGYLLETAHSRVA
                     WLSDTAGLPEKTLKFLLNNHPQVMVIDCSHPPRADAPRNHCDLNTVLALNQVIRSPQV
                     ILTHISHQFDAWLMENALPSGFEVGFDGMEIGVA"
     gene            complement(1646..2080)
                     /gene="phnO"
                     /locus_tag="ACHKTW_000195"
     CDS             complement(1646..2080)
                     /gene="phnO"
                     /locus_tag="ACHKTW_000195"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoalkylphosphonate N-acetyltransferase"
                     /protein_id="HFO6461291.1"
                     /translation="MPACELRPATQYDTDAVYALICELKQAEFDHHAFRVGFNANLRD
                     PNMRYHLALLDGEVVGMIGLHLQFHLHHVNWIGEIQELVVMPQARGLNVGSKLLAWAE
                     EEARQAGAEMTELSTNVKRHDAHRFYLREGYEQSHFRFTKAL"
     gene            complement(2067..2624)
                     /gene="phnN"
                     /locus_tag="ACHKTW_000196"
     CDS             complement(2067..2624)
                     /gene="phnN"
                     /locus_tag="ACHKTW_000196"
                     /EC_number="2.7.4.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033863 - ribose 1,5-bisphosphate
                     phosphokinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 1,5-bisphosphokinase"
                     /protein_id="HFO6461292.1"
                     /translation="MMGKLIWLMGPSGSGKDSLLAELRLREQTQLLVAHRYITRDASA
                     GSENHIALSEREFFTRAGQNLLALSWHANGLYYGVGIEIDLWLHAGFDVVVNGSRAHL
                     PQARARYQSALLPVCLQVSPEILRQRLENRGRENASEINARLARAARYTPQDCHTLNN
                     DGSLRQSVDTLLTLIHQKEKHHACL"
     gene            complement(2624..3760)
                     /gene="phnM"
                     /locus_tag="ACHKTW_000197"
     CDS             complement(2624..3760)
                     /gene="phnM"
                     /locus_tag="ACHKTW_000197"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HFO6461293.1"
                     /translation="MIINNVKLVLENEVVHGSLEVQDGEIRAFAESQSRLPEAMDGEG
                     GWLLPGLIELHTDNLDKFFTPRPKVDWPAHSAMSSHDALMVASGITTVLDAVAIGDVR
                     DGGDRLENLEKMINTIEETQKRGVNRAEHRLHLRCELPHHTTLPLFEKLVQREPVTLV
                     SLMDHSPGQRQFANREKYREYYQGKYSLTDAQMQQYEEEQLALAARWSQPNRESIAAL
                     CRARQIALASHDDATHAHVAESHQLGSVIAEFPTTFEAAEASRKHGMNVLMGAPNIVR
                     GGSHSGNVAASELAQLGLLDILSSDYYPASLLDAAFRVADDESNRFTLPQAVRLVTKN
                     PAQALNLQDRGVIGEGKRADLVLAHRQGNHIHIDHVWRQGKRVF"
     gene            complement(3757..4437)
                     /gene="phnL"
                     /locus_tag="ACHKTW_000198"
     CDS             complement(3757..4437)
                     /gene="phnL"
                     /locus_tag="ACHKTW_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HFO6461294.1"
                     /translation="MINVQNVSKTFILHQQNGVRLPVLQRASLTVNAGECVVLHGHSG
                     SGKSTLLRSLYANYLPDEGQIQIKHGDEWVDLVTAPARKVVEIRKTTIGWVSQFLRVI
                     PRISALEVVMQPLLDTGVPREACAAKAARLLTRLNVPERLWHLAPSTFSGGEQQRVNI
                     ARGFIVDYPILLLDEPTASLDAKNSAAVVELIHEAKARGAAIVGIFHDEAVRNDVADR
                     LHPMGASS"
     gene            complement(4542..5300)
                     /gene="phnK"
                     /locus_tag="ACHKTW_000199"
     CDS             complement(4542..5300)
                     /gene="phnK"
                     /locus_tag="ACHKTW_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnK"
                     /protein_id="HFO6461295.1"
                     /translation="MNQPLLSVNNLTHLYAPGKGFSDVSFDLWPGEVLGIVGESGSGK
                     TTLLKSISARLTPQQGEIHYESRSLYAMSEADRRRLLRTEWGVVHQHPLDGLRRQVSA
                     GGNIGERLMATGARHYGDIRATAQKWLEEVEIPANRIDDLPTTFSGGMQQRLQIARNL
                     VTHPKLVFMDEPTGGLDVSVQARLLDLLRGLVVELNLAVVIVTHDLGVARLLADRLLV
                     MKQGQVVESGLTDRVLDDPHHPYTQLLVSSVLQN"
     gene            complement(5297..6142)
                     /gene="phnJ"
                     /locus_tag="ACHKTW_000200"
     CDS             complement(5297..6142)
                     /gene="phnJ"
                     /locus_tag="ACHKTW_000200"
                     /EC_number="4.7.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HFO6461296.1"
                     /translation="MANLSGYNFAYLDEQTKRMIRRAILKAVAIPGYQVPFGGREMPM
                     PYGWGTGGIQLTASVIGESDVLKVIDQGADDTTNAVSIRNFFKRVTGVNTTERTDDAT
                     LIQTRHRIPETPLTEDQIIIFQVPIPEPLRFIEPRETETRTMHALEEYGVMQVKLYED
                     IARFGHIATTYAYPVKVNGRYVMDPSPIPKFDNPKMDMMPALQLFGAGREKRIYAVPP
                     FTRVESLDFDDHPFTVQQWDEPCAICGSTHSYLDEVVLDDAGNRMFVCSDTDYCRQQS
                     EAKNQ"
     gene            complement(6135..7199)
                     /gene="phnI"
                     /locus_tag="ACHKTW_000201"
     CDS             complement(6135..7199)
                     /gene="phnI"
                     /locus_tag="ACHKTW_000201"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate synthase subunit PhnI"
                     /protein_id="HFO6461297.1"
                     /translation="MYVAVKGGEKAIDAAHALQESRRRGDTDLPELSVAQIEQQLNLA
                     VDRVMTEGGIADRELAALALKQASGDNVEAIFLLRAYRTTLAKLAVSEPLDTTEMRLE
                     RRISAVYKDIPGGQLLGPTYDYTHRLLDFTLLANGEAPTLTTADSEQSPSPHVFSLLA
                     RQGLAKFEEDSGAQPDDITRTPPVYPCSRSSRLQQLMRGDEGYLLALAYSTQRGYGRN
                     HPFAGEIRSGYIDVSIVPEELGFAVNVGELLMTECEMVNGFIDPPDEPPHFTRGYGLV
                     FGMSERKAMAMALVDRALQAPEYGEHATGPAQDEEFVLAHADNVEAAGFVSHLKLPHY
                     VDFQAELELLKRLQQEQNHG"
     gene            complement(7199..7783)
                     /gene="phnH"
                     /locus_tag="ACHKTW_000202"
     CDS             complement(7199..7783)
                     /gene="phnH"
                     /locus_tag="ACHKTW_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HFO6461298.1"
                     /translation="MTLETAFMLPVQDAQHSFRRLLKAMSEPGVIVALHQLKRGWQPL
                     NIATTSVLLTLADNDTPVWLATPLSNDIVNQSLRFHTNAPLVSQPEQATFAVTDEAIS
                     SEQLNALSTGTAVAPEAGATLILQVASLSGGRMLRLTGAGIAEERMIAPQLPECILHE
                     LTERPHPFPLGIDLILTCGERLLAVPRTTHVEVC"
     gene            complement(7780..8232)
                     /gene="phnG"
                     /locus_tag="ACHKTW_000203"
     CDS             complement(7780..8232)
                     /gene="phnG"
                     /locus_tag="ACHKTW_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HFO6461299.1"
                     /translation="MHADTATRQHWMSVLAHSQPAELAARLKALNITADYEVIRAAET
                     GLVQIQARMGGTGERFFAGDATLTRAAVRLTDGTLGYSWVLGRDKQHAERCALIDALM
                     QQSRYFQNLSETVIAPLDADRMARIAARQAEVNASRVDFFTMVRGDNA"
     gene            complement(8233..8958)
                     /gene="phnF"
                     /locus_tag="ACHKTW_000204"
     CDS             complement(8233..8958)
                     /gene="phnF"
                     /locus_tag="ACHKTW_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism transcriptional regulator
                     PhnF"
                     /protein_id="HFO6461300.1"
                     /translation="MHLSTHPTSYPTRYQEIAAKLEQELRQHYRCGDYLPAEQQLAAR
                     FEVNRHTLRRAIDQLVEKGWVQRRQGVGVLVLMRPFDYPLNAQARFSQNLLDQGSHPT
                     SEKLLSVLRPASGHVADALGITEGENVIHLRTLRRVNGVALCLIDHYFADLTLWPTLQ
                     RFDSGSLHDFLREQTGIALRRSQTRISARRAQAKECQRLEIPNMSPLLCVRTLNHRDG
                     ESSPAEYSVSLTRADMIEFTMEH"
     gene            complement(8979..9758)
                     /gene="phnE"
                     /locus_tag="ACHKTW_000205"
     CDS             complement(8979..9758)
                     /gene="phnE"
                     /locus_tag="ACHKTW_000205"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HFO6461301.1"
                     /translation="MQTITIAPPKRSWFSLLSWAVVLAVLVVSWQGAEMAPLTLIKDG
                     GNMATFAADFFPPDFSQWQDYLTEMAVTLQIAVWGTALAVVLSIPFGLMSAENLVPWW
                     VYQPVRRLMDACRAINEMVFAMLFVVAVGLGPFAGVLALFIHTTGVLSKLLSEAVEAI
                     EPGPVEGIRATGANKLEEILYGVLPQVMPLLISYSLYRFESNVRSATVVGMVGAGGIG
                     VTLWEAIRGFQFQQTCALMVLIIVTVSLLDFLSQRLRKHFI"
     gene            complement(9864..10880)
                     /gene="phnD"
                     /locus_tag="ACHKTW_000206"
     CDS             complement(9864..10880)
                     /gene="phnD"
                     /locus_tag="ACHKTW_000206"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO6461302.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLSPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLIPGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEMQGVKSNKGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            complement(10905..11693)
                     /gene="phnC"
                     /locus_tag="ACHKTW_000207"
     CDS             complement(10905..11693)
                     /gene="phnC"
                     /locus_tag="ACHKTW_000207"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HFO6461303.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHGEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTGEQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQQFDNERFDHLYRSINRIEENAKAA"
     gene            complement(11826..12269)
                     /gene="yjdN"
                     /locus_tag="ACHKTW_000208"
     CDS             complement(11826..12269)
                     /gene="yjdN"
                     /locus_tag="ACHKTW_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HFO6461304.1"
                     /translation="MPLSPYLSFAGNCADAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAIPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVVKQQPTQ"
     gene            complement(12429..12764)
                     /gene="yjdM"
                     /locus_tag="ACHKTW_000209"
     CDS             complement(12429..12764)
                     /gene="yjdM"
                     /locus_tag="ACHKTW_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFO6461305.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            13166..15394
                     /gene="crfC"
                     /locus_tag="ACHKTW_000210"
     CDS             13166..15394
                     /gene="crfC"
                     /locus_tag="ACHKTW_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HFO6461306.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLAL
                     PWSQPNIAERHAMLNNELRKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDCLIQQLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRFENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARHELANNGKLPAPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQ
                     LWEDSLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQVEESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDALNAQLAALFQD
                     DSEPLSEMRTRCETLLQTAQNTISRDFTLRFAELESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGFHFPVFHGVVPHFNTRQLFSAVISRQDATDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFFAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQQTVMTHGWIYTDAQLLRDDIQTLFTAE
                     RY"
     gene            15391..16269
                     /gene="rdcB"
                     /locus_tag="ACHKTW_000211"
     CDS             15391..16269
                     /gene="rdcB"
                     /locus_tag="ACHKTW_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcZ-like family protein"
                     /protein_id="HFO6461307.1"
                     /translation="MTKTLLDGPGRVLESVYPRFLVDLAQGDDARLPQAHQQQFRERL
                     MQELLSRVQLQTWTNGGMLNAPLSLRLTMVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGEQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGVFGDACRLGSPQAVALLLGDLRVKATQHLAESINAAPTTR
                     HYYHQWFASSTVPTGGDHADFLSWLGKWTTADKQPVCWSVTQRWQTVALGMPRLCSAQ
                     RLAGAMVEEIFSVNLA"
     gene            16533..18035
                     /gene="proP"
                     /locus_tag="ACHKTW_000212"
     CDS             16533..18035
                     /gene="proP"
                     /locus_tag="ACHKTW_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HFO6461308.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKHWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFTGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVIGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDNIDHEIADLQAKRTRLVQQHPRIDE"
     gene            18147..18236
                     /gene="pmrR"
                     /locus_tag="ACHKTW_000213"
     CDS             18147..18236
                     /gene="pmrR"
                     /locus_tag="ACHKTW_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HFO6461309.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFASF"
     gene            complement(18212..19312)
                     /gene="pmrB"
                     /locus_tag="ACHKTW_000214"
     CDS             complement(18212..19312)
                     /gene="pmrB"
                     /locus_tag="ACHKTW_000214"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HFO6461310.1"
                     /translation="MNLMHFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQNEANQI"
     gene            complement(19313..19981)
                     /gene="basR"
                     /locus_tag="ACHKTW_000215"
     CDS             complement(19313..19981)
                     /gene="basR"
                     /locus_tag="ACHKTW_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HFO6461311.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEAGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            complement(19978..21624)
                     /gene="eptA"
                     /locus_tag="ACHKTW_000216"
     CDS             complement(19978..21624)
                     /gene="eptA"
                     /locus_tag="ACHKTW_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HFO6461312.1"
                     /translation="MLKRLLKRPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILVGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAALIACWIKIKPATSRLRSVLFLRGA
                     NILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLV
                     RIGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNT
                     ASCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGAC
                     DRVPHQNVTALNLPGQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYN
                     RYPPQFRKFTPTCDTNEIQTCSKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTS
                     LVYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQA
                     QTQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSE"
     gene            complement(21728..23065)
                     /gene="adiC"
                     /locus_tag="ACHKTW_000217"
     CDS             complement(21728..23065)
                     /gene="adiC"
                     /locus_tag="ACHKTW_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HFO6461313.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            complement(23202..23963)
                     /gene="adiY"
                     /locus_tag="ACHKTW_000218"
     CDS             complement(23202..23963)
                     /gene="adiY"
                     /locus_tag="ACHKTW_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HFO6461314.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            complement(24288..26555)
                     /gene="adiA"
                     /locus_tag="ACHKTW_000219"
     CDS             complement(24288..26555)
                     /gene="adiA"
                     /locus_tag="ACHKTW_000219"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HFO6461315.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSTHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKRHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPVAEVTPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHVMCVKA"
     gene            complement(26754..27662)
                     /gene="melR"
                     /locus_tag="ACHKTW_000220"
     CDS             complement(26754..27662)
                     /gene="melR"
                     /locus_tag="ACHKTW_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HFO6461316.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGHITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSGWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            27945..29300
                     /gene="melA"
                     /locus_tag="ACHKTW_000221"
     CDS             27945..29300
                     /gene="melA"
                     /locus_tag="ACHKTW_000221"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFO6461317.1"
                     /translation="MMTAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALEDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICEDMTEVCPDATMLNYVNPMAMNTWAM
                     YARYPHIKQVGLCHSVQGTAEELARDLNIDPATLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKPGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPGWLH
                     R"
     gene            29403..30824
                     /gene="melB"
                     /locus_tag="ACHKTW_000222"
     CDS             29403..30824
                     /gene="melB"
                     /locus_tag="ACHKTW_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HFO6461318.1"
                     /translation="MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLV
                     GTLFLVARIWDAINDPIMGWIVNATRSRWGKFKPWILIGTLANSVILFLLFSAHLFEG
                     TTQIVFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTA
                     GVTLPFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQPSAEGSHL
                     TLKAIVALIYKNDQLSCLLGMALAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAG
                     AANLVTLVFFPRLVKSLSRRILWAGASILPVLSCGVLLLMALMSYHNVVLIVIAGILL
                     NVGTALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFFIAVVLG
                     MIGYVPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQIHLLDK
                     YRKVPPEPVHADIPVGAVSDVKA"
     gene            complement(30963..31592)
                     /gene="yjdF"
                     /locus_tag="ACHKTW_000223"
     CDS             complement(30963..31592)
                     /gene="yjdF"
                     /locus_tag="ACHKTW_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HFO6461319.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLMEVTPVIIV
                     VPLLLATAKRYPLTPLLYTLIFFHAIILMVGGQYTYAKVPVGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVIFLARFHCRQLRRFGLITG"
     gene            complement(31715..33361)
                     /gene="fumB"
                     /locus_tag="ACHKTW_000224"
     CDS             complement(31715..33361)
                     /gene="fumB"
                     /locus_tag="ACHKTW_000224"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFO6461320.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRKGIW
                     IEKLEHNPGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            complement(33439..34779)
                     /gene="dcuB"
                     /locus_tag="ACHKTW_000225"
     CDS             complement(33439..34779)
                     /gene="dcuB"
                     /locus_tag="ACHKTW_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HFO6461321.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            complement(35350..36069)
                     /gene="dcuR"
                     /locus_tag="ACHKTW_000226"
     CDS             complement(35350..36069)
                     /gene="dcuR"
                     /locus_tag="ACHKTW_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HFO6461322.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEMIF
                     NSDTPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            complement(36066..37697)
                     /gene="dcuS"
                     /locus_tag="ACHKTW_000227"
     CDS             complement(36066..37697)
                     /gene="dcuS"
                     /locus_tag="ACHKTW_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFO6461323.1"
                     /translation="MRHSLPYRMLRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILKALNGKENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            37878..38108
                     /gene="yjdI"
                     /locus_tag="ACHKTW_000228"
     CDS             37878..38108
                     /gene="yjdI"
                     /locus_tag="ACHKTW_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S mono-cluster protein YjdI"
                     /protein_id="HFO6461324.1"
                     /translation="MDQALLDEGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKPWIMPDEVDVATVVKVIDTCPSGALKYRHK"
     gene            38120..38392
                     /gene="yjdJ"
                     /locus_tag="ACHKTW_000229"
     CDS             38120..38392
                     /gene="yjdJ"
                     /locus_tag="ACHKTW_000229"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO6461325.1"
                     /translation="MEIREGHNKFYINDEQGKQIAEIVFVPTGENLAIIEHTDVDESL
                     KGQGIGKQLVAKVVEKMRREKRKIIPLCPFAKHEFDKTREYDDIRS"
     gene            38619..38915
                     /gene="ghoS"
                     /locus_tag="ACHKTW_000230"
     CDS             38619..38915
                     /gene="ghoS"
                     /locus_tag="ACHKTW_000230"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HFO6461326.1"
                     /translation="MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEY
                     GIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDIDFDLNIMTVDDYFRQFYK"
     gene            38943..39116
                     /gene="ghoT"
                     /locus_tag="ACHKTW_000231"
     CDS             38943..39116
                     /gene="ghoT"
                     /locus_tag="ACHKTW_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system toxin GhoT"
                     /protein_id="HFO6461327.1"
                     /translation="MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITW
                     PMSLPVALLFSLF"
     gene            complement(39235..40752)
                     /gene="lysS"
                     /locus_tag="ACHKTW_000232"
     CDS             complement(39235..40752)
                     /gene="lysS"
                     /locus_tag="ACHKTW_000232"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFO6461328.1"
                     /translation="MSEQETRGANEAIDFNDELRNRREKLAALRQQGVAFPNDFRRDH
                     TSDQLHEEFDAKDNQELESLNIEVSVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     SLPEGVYNDQFKKWDLGDIIGARGTLFKTQTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEVRYRQRYLDLIANDKSRQTFVVRSKILAAIRQFMVARGFMEVETPMMQVIPGGA
                     SARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYHDLIELTESLFRTLAQEVLGTTKVTYGEHVFDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDAAKALAESIGITVEKSWGLGRIVTEIFDEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAERFQEQVNAKAAG
                     DDEAMFYDEDYVTALEYGLPPTAGLGIGIDRMIMLFTNSHTIRDVILFPAMRPQK"
     gene            complement(40989..42446)
                     /gene="dtpC"
                     /locus_tag="ACHKTW_000233"
     CDS             complement(40989..42446)
                     /gene="dtpC"
                     /locus_tag="ACHKTW_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpC"
                     /protein_id="HFO6461329.1"
                     /translation="MKTPSQPRAIYYIVAIQIWEYFSFYGMRALLILYLTHQLGFDDN
                     HAISLFSAYASLVYVTPILGGWLADRLLGNRTAVIAGALLMTLGHVVLGIDTNSTFSL
                     YLALAIIICGYGLFKSNISCLLGELYDENDHRRDGGFSLLYAAGNIGSIAAPIACGLA
                     AQWYGWHVGFALAGGGMFIGLLIFLSGHRHFQSTRSMDKKALTSVKFALPVWSWLVVM
                     LCLAPVFFTLLLENDWSGYLLAIVCLIAAQIIARMMIKFPEHRRALWQIVLLMFVGTL
                     FWVLAQQGGSTISLFIDRFVNRQAFNIEVPTALFQSVNAIAVMLAGVVLAWLASPESR
                     GNSTLRVWLKFAFGLLLMACGFMLLAFDARHAAADGQASMGVMISGLALMGFAELFID
                     PVAIAQITRLKMSGVLTGIYMLATGAVANWLAGVVAQQTTESQISGMAIAAYQRFFSQ
                     MGEWTLACVAIIVVLAFATRFLFSTPTNMIQESND"
     gene            complement(42505..44652)
                     /gene="cadA"
                     /locus_tag="ACHKTW_000234"
     CDS             complement(42505..44652)
                     /gene="cadA"
                     /locus_tag="ACHKTW_000234"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase CadA"
                     /protein_id="HFO6461330.1"
                     /translation="MNVIAILNHMGVYFKEEPIRELHRALERLNFQIVYPNDRDDLLK
                     LIENNARLCGVIFDWDKYNLELCEEISKMNENLPLYAFANTYSTLDVSLNDLRLQISF
                     FEYALGAAEDIANKIKQTTDEYINTILPPLTKALFKYVREGKYTFCTPGHMGGTAFQK
                     SPVGSLFYDFFGPNTMKSDISISVSELGSLLDHSGPHKEAEQYIARVFNADRSYMVTN
                     GTSTANKIVGMYSAPAGSTILIDRNCHKSLTHLMMMSDVTPIYFRPTRNAYGILGGIP
                     QSEFQHATIAKRVKETPNATWPVHAVITNSTYDGLLYNTDFIKKTLDVKSIHFDSAWV
                     PYTNFSPIYEGKCGMSGGRVEGKVIYETQSTHKLLAAFSQASMIHVKGDVNEETFNEA
                     YMMHTTTSPHYGIVASTETAAAMMKGNAGKRLINGSIERAIKFRKEIKRLRTESDGWF
                     FDVWQPDHIDTTECWPLRSDSTWHGFKNIDNEHMYLDPIKVTLLTPGMEKDGTMSDFG
                     IPASIVAKYLDEHGIVVEKTGPYNLLFLFSIGIDKTKALSLLRALTDFKRAFDLNLRV
                     KNMLPSLYREDPEFYENMRIQELAQNIHKLIVHHNLPDLMYRAFEVLPTMVMTPYAAF
                     QKELHGMTEEVYLDEMVGRINANMILPYPPGVPLVMPGEMITEESRPVLEFLQMLCEI
                     GAHYPGFETDIHGAYRQADGRYTVKVLKEESKK"
     gene            complement(44732..46066)
                     /gene="cadB"
                     /locus_tag="ACHKTW_000235"
     CDS             complement(44732..46066)
                     /gene="cadB"
                     /locus_tag="ACHKTW_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043872 - lysine:cadaverine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cadaverine/lysine antiporter"
                     /protein_id="HFO6461331.1"
                     /translation="MSSAKKIGLFACTGVVAGNMMGSGIALLPANLASIGGIAIWGWI
                     ISIIGAMSLAYVYARLATKNPQQGGPIAYAGEISPAFGFQTGVLYYHANWIGNLAIGI
                     TAVSYLSTFFPVLNDPVPAGIACIAIVWVFTFVNMLGGTWVSRLTTIGLVLVLIPVVM
                     TAIVGWHWFDAATYAANWNTADTTDGHAIIKSILLCLWAFVGVESAAVSTGMVKNPKR
                     TVPLATMLGTGLAGIVYIAATQVLSGMYPSSVMAASGAPFAISASTILGNWAAPLVSA
                     FTAFACLTSLGSWMMLVGQAGVRAANDGNFPKVYGEVDSNGIPKKGLLLAAVKMTALM
                     ILITLMNSAGGKASDLFGELTGIAVLLTMLPYFYSCVDLIRFEGVNIRNFVSLICSVL
                     GCVFCFIALMGASSFELAGTFIVSLIILMFYARKMHERQSHSMDNHTASNAH"
     gene            complement(46432..47970)
                     /gene="cadC"
                     /locus_tag="ACHKTW_000236"
     CDS             complement(46432..47970)
                     /gene="cadC"
                     /locus_tag="ACHKTW_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylation/transport transcriptional
                     activator CadC"
                     /protein_id="HFO6461332.1"
                     /translation="MQQPVVRVGEWLVTPSINQISRNGRQLTLEPRLIDLLVFFAQHS
                     GEVLSRDELIDNVWKRSIVTNHVVTQSISELRKSLKDNDEDSPVYIATVPKRGYKLMV
                     PVIWYSEEEGEEIMLSSPPPIPEAVPATDSPSHSLNIQNTATPPEQSPVKSKRFTTFW
                     VWFFFLLSLGICVALVAFSTLDTRLPMSKSRILLNPRDIDINMVNKSCNSWSSPYQLS
                     YAIGVGDLVATSLNTFSTFMVHDKINYNIDEPSSSGKTLSIAFVNQRQYRAQQCFMSI
                     KLVDNADGSTMLDKRYVITNGNQLAIQNDLLESLSKALNQPWPQRMQETLQQILPHRG
                     ALLTNFYQAHDYLLHGDDKSLNRASELLGEIVQSSPEFTYARAEKALVDIVRHSQHPL
                     DEKQLAALNTEIDNIVTLPELNNLSIIYQIKAVSALVKGKTDESYQAINTGIDLEMSW
                     LNYVLLGKVYEMKGMNREAADAYLTAFNLRPGANTLYWIENGIFQTSVPYVVPYLDKF
                     LASE"
     gene            complement(48230..48389)
                     /locus_tag="ACHKTW_000237"
                     /pseudo
     CDS             complement(48230..48389)
                     /locus_tag="ACHKTW_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218818.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            complement(48587..48662)
                     /locus_tag="ACHKTW_000238"
     tRNA            complement(48587..48662)
                     /locus_tag="ACHKTW_000238"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(48627..48629),aa:Phe,seq:gaa)
     gene            complement(48769..49344)
                     /gene="yjdC"
                     /locus_tag="ACHKTW_000239"
     CDS             complement(48769..49344)
                     /gene="yjdC"
                     /locus_tag="ACHKTW_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO6461333.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVSRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            complement(49381..51078)
                     /gene="dsbD"
                     /locus_tag="ACHKTW_000240"
     CDS             complement(49381..51078)
                     /gene="dsbD"
                     /locus_tag="ACHKTW_000240"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HFO6461334.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITSEHAKIADVQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVTNNAASQPVSVPQQEQPT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPGGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILVLPVFLLERVIGDIWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            complement(51054..51392)
                     /gene="cutA"
                     /locus_tag="ACHKTW_000241"
     CDS             complement(51054..51392)
                     /gene="cutA"
                     /locus_tag="ACHKTW_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HFO6461335.1"
                     /translation="MLDEKSSNTASVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            complement(51508..52809)
                     /gene="dcuA"
                     /locus_tag="ACHKTW_000242"
     CDS             complement(51508..52809)
                     /gene="dcuA"
                     /locus_tag="ACHKTW_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HFO6461336.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            complement(52927..54363)
                     /gene="aspA"
                     /locus_tag="ACHKTW_000243"
     CDS             complement(52927..54363)
                     /gene="aspA"
                     /locus_tag="ACHKTW_000243"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008797 - aspartate ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HFO6461337.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            54700..55176
                     /gene="fxsA"
                     /locus_tag="ACHKTW_000244"
     CDS             54700..55176
                     /gene="fxsA"
                     /locus_tag="ACHKTW_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HFO6461338.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDERDRLDHKDDRQD"
     gene            complement(55192..56448)
                     /gene="yjeH"
                     /locus_tag="ACHKTW_000245"
     CDS             complement(55192..56448)
                     /gene="yjeH"
                     /locus_tag="ACHKTW_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HFO6461339.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAALVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTRGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPASIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEQMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLAGLWLFLPKRKTPENGITT"
     gene            56724..57017
                     /gene="groS"
                     /locus_tag="ACHKTW_000246"
     CDS             56724..57017
                     /gene="groS"
                     /locus_tag="ACHKTW_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFO6461340.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            57061..58707
                     /gene="groL"
                     /locus_tag="ACHKTW_000247"
     CDS             57061..58707
                     /gene="groL"
                     /locus_tag="ACHKTW_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFO6461341.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            58845..59198
                     /gene="yjeI"
                     /locus_tag="ACHKTW_000248"
     CDS             58845..59198
                     /gene="yjeI"
                     /locus_tag="ACHKTW_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HFO6461342.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(59401..60269)
                     /gene="yjeJ"
                     /locus_tag="ACHKTW_000249"
                     /pseudo
     CDS             complement(59401..60269)
                     /gene="yjeJ"
                     /locus_tag="ACHKTW_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
     gene            complement(60659..61687)
                     /gene="epmB"
                     /locus_tag="ACHKTW_000250"
     CDS             complement(60659..61687)
                     /gene="epmB"
                     /locus_tag="ACHKTW_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0072581 - protein-N6-(L-lysyl)-L-lysine
                     modification to protein-N6-(beta-lysyl)-L-lysine [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HFO6461343.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVAPGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAKTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
     gene            61729..62295
                     /gene="efp"
                     /locus_tag="ACHKTW_000251"
     CDS             61729..62295
                     /gene="efp"
                     /locus_tag="ACHKTW_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFO6461344.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            62347..62472
                     /gene="ecnA"
                     /locus_tag="ACHKTW_000252"
     CDS             62347..62472
                     /gene="ecnA"
                     /locus_tag="ACHKTW_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HFO6461345.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            62583..62729
                     /gene="ecnB"
                     /locus_tag="ACHKTW_000253"
     CDS             62583..62729
                     /gene="ecnB"
                     /locus_tag="ACHKTW_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HFO6461346.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            62904..63221
                     /gene="sugE"
                     /locus_tag="ACHKTW_000254"
     CDS             62904..63221
                     /gene="sugE"
                     /locus_tag="ACHKTW_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HFO6461347.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(63218..63751)
                     /gene="blc"
                     /locus_tag="ACHKTW_000255"
     CDS             complement(63218..63751)
                     /gene="blc"
                     /locus_tag="ACHKTW_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HFO6461348.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(63840..64973)
                     /gene="ampC"
                     /locus_tag="ACHKTW_000256"
     CDS             complement(63840..64973)
                     /gene="ampC"
                     /locus_tag="ACHKTW_000256"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HFO6461349.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPTTKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPAEEKNYAWG
                     YREGKAVHVSPGALDAKTYGVKSTIEDMACWVRSNMNPRDINDKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSGNKIALAAHPVKAITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAAWQILNALQ"
     gene            complement(65036..65395)
                     /gene="frdD"
                     /locus_tag="ACHKTW_000257"
     CDS             complement(65036..65395)
                     /gene="frdD"
                     /locus_tag="ACHKTW_000257"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HFO6461350.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGIVTI"
     gene            complement(65406..65801)
                     /gene="frdC"
                     /locus_tag="ACHKTW_000258"
     CDS             complement(65406..65801)
                     /gene="frdC"
                     /locus_tag="ACHKTW_000258"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HFO6461351.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            complement(65812..66546)
                     /gene="frdB"
                     /locus_tag="ACHKTW_000259"
     CDS             complement(65812..66546)
                     /gene="frdB"
                     /locus_tag="ACHKTW_000259"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HFO6461352.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            complement(66539..68347)
                     /gene="frdA"
                     /locus_tag="ACHKTW_000260"
     CDS             complement(66539..68347)
                     /gene="frdA"
                     /locus_tag="ACHKTW_000260"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HFO6461353.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            68672..69649
                     /gene="epmA"
                     /locus_tag="ACHKTW_000261"
     CDS             68672..69649
                     /gene="epmA"
                     /locus_tag="ACHKTW_000261"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HFO6461354.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            69868..71370
                     /gene="yjeM"
                     /locus_tag="ACHKTW_000262"
     CDS             69868..71370
                     /gene="yjeM"
                     /locus_tag="ACHKTW_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HFO6461355.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     SAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVVFAIF
                     AYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVLSNGS
                     VNLGNITYVLMKSLGVTLGNAQHLSPEASLSLGVWFARITGLSMFLAYTGAFFTLCYS
                     PLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLVTVFILLVSFGGGTASAFFNK
                     LTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTHLSAMIATVVVVLVVTFANV
                     FTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRVAKNPQWAVE"
     gene            71422..71736
                     /gene="yjeN"
                     /locus_tag="ACHKTW_000263"
     CDS             71422..71736
                     /gene="yjeN"
                     /locus_tag="ACHKTW_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjeN"
                     /protein_id="HFO6461356.1"
                     /translation="MDDTSRDPAITEDEIRALQFSAGDVAEIEQTILSFVDACHTRKV
                     AMVVGSTINTLKDRDGKRWGNLLDIYCAYLIRCLVFRGELVGYGDLFHMRYSEIKRPV
                     TL"
     gene            71733..72047
                     /gene="yjeO"
                     /locus_tag="ACHKTW_000264"
     CDS             71733..72047
                     /gene="yjeO"
                     /locus_tag="ACHKTW_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeO family protein"
                     /protein_id="HFO6461357.1"
                     /translation="MSARMFVLCCIWFIVAFLWITITSALDKEWMIDGRGINNVCDVL
                     MYLEEDDIRDVGVIMTLPLFFPFLWFALWRKKRGWFMYATALAIFGYWLWQFFLRYQF
                     CL"
     gene            complement(72078..75401)
                     /gene="mscM"
                     /locus_tag="ACHKTW_000265"
     CDS             complement(72078..75401)
                     /gene="mscM"
                     /locus_tag="ACHKTW_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HFO6461358.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VIEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNMRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDRE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(75423..76391)
                     /gene="asd"
                     /locus_tag="ACHKTW_000266"
     CDS             complement(75423..76391)
                     /gene="asd"
                     /locus_tag="ACHKTW_000266"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HFO6461359.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMANLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            complement(76488..77540)
                     /gene="rsgA"
                     /locus_tag="ACHKTW_000267"
     CDS             complement(76488..77540)
                     /gene="rsgA"
                     /locus_tag="ACHKTW_000267"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HFO6461360.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            77635..78180
                     /gene="orn"
                     /locus_tag="ACHKTW_000268"
     CDS             77635..78180
                     /gene="orn"
                     /locus_tag="ACHKTW_000268"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HFO6461361.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEAYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            complement(78193..>78415)
                     /locus_tag="ACHKTW_000269"
     CDS             complement(78193..>78415)
                     /locus_tag="ACHKTW_000269"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6461362.1"
                     /translation="LYQLSYSRVLLNFAFVIFAFLRRYGRRIIREIFPSATPQKSFFC
                     VFISSAEIISKTFNLASKSRRRKWIPALT"
BASE COUNT        18770 a        19687 c        20628 g        19330 t
ORIGIN      
        1 caaatactga gcacaattta tcaatgcaac cggtgtttct ttttaaaaaa cgtaccggag
       61 caatgggcgg tcatgtcgct ggcaccgcag gattctgcgt tgctgtgcca gccttttatt
      121 catggtcaat gagtgaggtg attgataaag acaccaggtc gtcagagcgc actgattatc
      181 cgcattatgc cagtgaagtt gcactggctt ttggttctcg agtggttggc ctcgaactgg
      241 caaaaatgat tgtggatgcg tggctgggcg cacagtacga aggcggtcgt catcaacaac
      301 gcgtggaggc gattacggca atagagcagc ggagaaattg agattcatcc actacttgca
      361 tggatgagta atgactaatg tggatagagt ttctttttga ggttggctaa tgaaacgctt
      421 tccgctattt cttttattca ccctgctcac gctgtccacc gttccggccc aggctgatat
      481 tatcgacgat actattggta atattcagca ggcgattaat gacgcttata accctgaccg
      541 tgggcgagac tatgaagatt cgcgcgacga cggctggcag cgtgaagtga gcgacgatcg
      601 gcgcagacaa tatgacgacc gccgccgcca gtttgaagat cgccgcaggc agctggacga
      661 tcgccagcgc cagcttgatc aggagcgtcg gcaactggag gatgaagagc ggagaatgga
      721 agatgagtat gggcgatgag gttgggtatg gagcaggcat cgcccctcac cccaatgggg
      781 agagggagaa aacgagcgca atattcaata ttgagcacaa tcggtcccct cgcccctctg
      841 gggagagggt tagggtgagg gggccagcgc gcacattccc tctcatcacg ccaccccaat
      901 ctccatccca tcaaacccca cctcaaaccc tgacggtagt gcgttttcca tcagccacgc
      961 atcaaactgg tggctgatat gggtcagaat cacctgtggc gagcggataa cctgattcag
     1021 cgcaagcacg gtatttaaat cacagtgatt acgcggtgca tccgcgcgcg gcgggtgact
     1081 gcaatcaatc accatcacct gcggatgatt attgagtaaa aatttcagcg ttttttccgg
     1141 caggccagcg gtgtcagaca gccacgccac ccggctatgt gccgtttcca gcagataacc
     1201 gaaggtcagt tttgagtggt taagcggcag cggcgtgacc tgcaacccct gcaaatcaaa
     1261 caccacaaac ggttccactg tgtggctgaa atcaagcagg cccggatgtt taaacagatc
     1321 gtcgcagccc tgttcatccg gcgggccgta gaccgggatc acatcgccca cgccccagcg
     1381 tagcggaaat agcccttgga cgtgatccat atggtagtgc gtcagcaaaa actgctggaa
     1441 cgatccaggc gaccagcgat cggtgagatc gtgccgcccg gcgtcgatca gggtgatcgc
     1501 gtcgttaaac ttcactacgc cgctgcatgg ctggcggcga tactgcggcg agcgccgcgc
     1561 tctggcgcag gccgcgcact cgcagcccca tgccggaacg ccctgtgcgc cgccagtgcc
     1621 ggtgagcgtg agggtcaggc tcatgttaca gcgccttggt gaagcggaag tggctctgct
     1681 cgtagccttc acgcaggtaa aaacggtgtg cgtcgtggcg cttcacgttg gtggaaagct
     1741 cggtcatttc ggccccggcc tggcgggctt cttcttctgc ccatgccagt aatttactgc
     1801 cgacgttcag accgcgcgcc tgcggcatca ccaccagttc ctgaatttcg ccgatccagt
     1861 tgacatgatg cagatgaaac tgcaaatgca ggccgatcat gccgacaact tcgccatcca
     1921 gcagcgccag atggtagcgc atgtttgggt cgcgcaggtt ggcgttaaag cccacgcgaa
     1981 acgcatggtg gtcaaactcc gcctgtttta gctcacaaat cagcgcgtaa accgcgtcgg
     2041 tatcgtactg cgtggccggg cgaagctcac aagcaggcat ggtgtttctc cttctgatgg
     2101 atcagcgtca gcagcgtgtc gaccgactgg cgcaggctgc cgtcattgtt gagcgtatgg
     2161 caatcctgtg gagtatagcg ggcggcgcgc gccaggcggg cgttaatttc actggcgttt
     2221 tcacggccac ggttttccag gcgctggcgc aggatctccg gcgaaacctg taaacagacg
     2281 ggcagcagcg ccgattgata gcgcgcccgt gcctgcggca aatgcgcgcg tgaaccgttg
     2341 acgactacgt cgaatccagc gtgcagccag aggtcaatct cgatgccgac gccataatac
     2401 agaccgttag cgtgccagct taaggccagc aggttttgcc ccgcgcgggt aaaaaactcc
     2461 cgctcactca gggcgatatg gttttcactt ccggcgctgg cgtcgcgcgt gatgtagcga
     2521 tgcgccacca gtaactgcgt ttgttcccgc aggcggagtt ccgccagcag gctgtctttc
     2581 ccggagccgg acggccccat taaccaaatc agttttccca tcatcagaac acccttttac
     2641 cctgacgcca gacgtggtcg atatgaatgt gattgccctg gcgatgcgcc agcaccaggt
     2701 cagcgcgttt accctcacca atcaccccgc gatcctgaag attcagcgcc tgcgccggat
     2761 ttttggtcac cagcctcacc gcctgcggta gcgtaaagcg gttgctctcg tcatcggcga
     2821 cgcggaacgc cgcatccagc aggctggcgg ggtagtagtc ggaagagagg atatccagca
     2881 gaccaagctg cgccagttca ctggccgcca cattgccgga gtgcgagccg ccacgcacga
     2941 tatttggcgc acccatcagc acgttcatgc catgcttacg cgaggcttcc gccgcttcga
     3001 acgtggtggg aaattcggcg atcacgctgc caagttggtg ggattcggca acgtgggcgt
     3061 gcgtggcgtc atcgtggctg gcgagcgcaa tttgtcgcgc gcggcacagg gcggcgatgg
     3121 attcgcgatt cggctgcgac cagcgtgcgg cgagcgccaa ttgctcttct tcgtactgct
     3181 gcatctgcgc atcggtgagg gagtatttgc cctgataata ttcgcgatac ttctcgcggt
     3241 tagcgaactg gcgctggccc ggtgagtggt ccatcagcga caccagcgtc accggttcgc
     3301 gctgaaccag tttttcaaac agcggcagtg tggtgtgatg cggcagttcg cagcgcaggt
     3361 gcaggcggtg ctcggcgcgg ttgacgccgc gtttctgcgt ctcttcgatg gtgttgatca
     3421 tcttctccag attctccagc cgatcgccgc cgtcgcgcac gtcgccaatc gccaccgcgt
     3481 ccagcacggt ggtgatgccg cttgccacca tcagcgcgtc gtggctgctc atcgccgaat
     3541 gggcgggcca gtcgactttc gggcgcgggg tgaagaattt atcgaggttg tcggtatgca
     3601 gctcaatcag gccgggtagt agccagccac cttcgccgtc catcgcttct ggcaggcggc
     3661 tctggctttc ggcaaaggcg cggatttcgc catcctgcac ctccagcgaa ccgtgcacca
     3721 cctcgttttc cagcaccagc ttaacgttat tgataatcat gaagaggctc ccattgggtg
     3781 caggcggtcg gcgacgtcat tacgtacagc ttcgtcatgg aagatgccga cgatggctgc
     3841 gccacgggct ttggcttcgt gaatcagttc caccaccgcg gcgctgtttt tggcgtccag
     3901 cgaggcggta ggttcgtcga gcagcagaat ggggtagtcg acgataaagc cgcgggcgat
     3961 gttgacgcgc tgctgttcgc cgccggagaa ggtcgatggt gccaggtgcc acaggcgttc
     4021 cggcacgttc aggcgggtga gaagacgcgc ggctttagcg gcgcaggctt cacgcggaac
     4081 gcctgtatcg agcagcggct gcatcaccac ttccagtgct gagatacgcg ggatgacgcg
     4141 cagaaactgg ctcacccagc cgatggtagt tttgcggatc tccaccactt tgcgcgctgg
     4201 cgcggtgacc aggtctaccc actcgtcacc gtgtttgatc tggatttgac cttcgtcggg
     4261 tagatagttg gcgtacagcg agcgcagcag ggttgatttg ccgctgccgg agtgaccgtg
     4321 gagcaccacg cattcgcctg cgttgacggt gagcgaggcg cgttgtaaga cgggcaggcg
     4381 cacgccgttt tgctggtgca ggatgaaggt tttactgacg ttttgtacgt taatcatttt
     4441 tcgcctcgtg gttcgcatat tggggagcgg tgcgcgcatt gcccctcacc ctaaccctct
     4501 ccccgaaggg gcgaggggac cgattgtgcg cggtgttgca ttcaattctg caaaaccgat
     4561 gacaccagca gttgtgtata cggatggtgc gggtcgtcga gcacgcggtc ggttaaccca
     4621 ctctccacca cttgcccctg cttcatcacc agcaaacggt ccgccagcag gcgggcgacg
     4681 cctaaatcgt gggtgacaat cactaccgcg aggttcagct ccaccaccag gccgcgcagc
     4741 aggtcgagca ggcgtgcctg taccgacaca tccagtccgc cggtcggctc gtccataaac
     4801 accagcttcg gatgcgtcac caggttgcgg gcgatctgca aacgttgctg catgccgccg
     4861 gaaaaggtgg tcggcaggtc gtcgatccgg ttggcgggaa tttccacctc ttccagccac
     4921 ttctgcgcgg tggcacgaat atcgccgtaa tgacgtgccc cggtcgccat cagccgctcg
     4981 ccgatattgc cgcctgccga cacctggcgg cgcaggccgt cgagcggatg ctgatgcact
     5041 acgccccatt cggtgcgcag caggcgacgg cggtcggcct cgctcattgc gtacagcgaa
     5101 cggctctcat agtgaatctc cccctgctgc ggcgtcaggc gcgcggagat tgatttcagc
     5161 agcgtggtct tcccggagcc ggactccccg acaatgccca gcacttcccc cggccataaa
     5221 tcaaaagaga catcgctaaa gcctttgccc ggcgcgtaaa ggtgggtcag gttattgacc
     5281 gaaagtaacg gttgattcat tggttttttg cctcgctctg ttggcggcaa taatcggtat
     5341 cggagcagac aaacatgcgg tttccggcgt catccagcac cacttcatca agatagctgt
     5401 gggtcgatcc gcagatggcg catggctcat cccactgctg aacggtgaac gggtgatcgt
     5461 cgaaatcgag actttccacg cgggtaaacg gcggcaccgc atagatgcgc ttctcgcgcc
     5521 ccgcgccgaa cagttgcagg gcgggcatca tgtccatttt tgggttatcg aatttcggga
     5581 tcggcgacgg gtccattacg tagcgcccgt tcaccttcac cggataggcg taggtagtgg
     5641 cgatatgacc gaagcgggcg atatcttcat acagtttcac ctgcatcacg ccgtactctt
     5701 ccagcgcgtg catggtgcgg gtttccgttt cgcgcggctc gataaagcgc agcggttcgg
     5761 ggatcggcac ctggaagata atgatctgat cttccgtcag cggcgtttcg ggaatgcggt
     5821 ggcgcgtctg gataagcgtc gcatcatccg tacgttcagt ggtgttcacc ccggtcacgc
     5881 gcttaaagaa gttgcgaatc gacacggcgt tggtggtgtc atccgcgccc tggtcaatca
     5941 ccttcaacac gtcgctttcg ccaatcacgc tggcggtgag ctgtatgccg ccggttcccc
     6001 agccgtatgg catgggcatc tcgcgcccgc caaacggcac ctgataaccg gggatcgcca
     6061 ccgcttttaa gatggcgcgg cggatcatgc gtttggtctg ctcgtcgagg taggcaaagt
     6121 tgtagccgct cagattagcc atggttctgc tcctgttgca gacgtttgag tagctccagt
     6181 tcggcctgga aatcgacgta gtgcgggagt ttgaggtgcg agacaaagcc tgcggcttcg
     6241 acgttgtcgg catgcgccag cacgaactct tcatcctgcg ccgggcctgt cgcgtgctcg
     6301 ccgtactccg gagcctgcaa cgcacggtca accagcgcca tcgccatcgc tttgcgctcg
     6361 ctcatgccga acaccagccc gtagccgcgc gtgaagtgcg gcggctcatc cggcgggtca
     6421 ataaaaccgt tgaccatttc acactcggtc atcagcagtt cgccgacgtt caccgcaaat
     6481 cccagctctt ccggcacaat cgacacgtcg atgtaaccac tgcggatttc gcccgcgaac
     6541 gggtgattgc gtccgtaacc gcgctgggta gagtaggcca gcgccagcaa atagccttcg
     6601 tcgccgcgca tcaactgctg caaacgggaa gaacgtgaac aggggtaaac cggcggcgtg
     6661 cgggtgatgt catccggctg tgcgccgcta tcctcttcaa acttcgccag cccctgacgc
     6721 gccagcaggc tgaaaacgtg cggcgacggg gattgttcgc tgtctgcggt ggtcagcgtc
     6781 ggcgcttcgc cgtttgccag cagggtgaaa tcgagcaggc gatgggtgta gtcgtaggtt
     6841 gggccaagca actgaccgcc gggaatatct ttataaaccg ctgaaatacg ccgttcgaga
     6901 cgcatctccg tggtgtcgag cggctcgctt accgccagct tcgccagcgt ggtgcggtag
     6961 gcgcgtagca ggaaaatcgc ttcgacgtta tcgccgctgg cctgtttcag cgccagcgcc
     7021 gccagttcgc ggtcggcaat gccgccttcg gtcatcacgc gatctaccgc gaggttaagc
     7081 tgctgttcaa tctgggcgac gctcagttcg ggcaaatcgg tatcgccccg gcgtcggctc
     7141 tcttgcaggg cgtgggcggc gtcgatcgcc ttttcgcccc ctttcacggc aacgtacatc
     7201 agcacacctc cacatgagtg gttcgcggaa cagccagcag gcgctcgccg caggtcagga
     7261 tcaggtcgat gccgagcggg aacgggtgcg ggcgctcggt gagttcgtgc agaatgcact
     7321 ccggcagctg cggagcgatc attcgttctt cggcaatgcc cgcgccggtg agacgcaaca
     7381 tgcgtccgcc gctcaggctg gcgacctgta aaatcagcgt cgcgcccgct tccggcgcaa
     7441 cggcggtgcc ggtggaaagg gcgttgagct gttcgctgga aatcgcctca tccgtcaccg
     7501 cgaaggtcgc ctgttccggc tggctgacca gcggcgcgtt ggtatgaaaa cgcaggctct
     7561 gattgacgat atcgttactt aatggcgtgg caagccacac cggcgtgtcg ttatcggcca
     7621 gcgtcagcag cacgctggtg gtggcgatat tcagcggctg ccagccgcgt ttgagctgat
     7681 gcagggcgac aatcacgccc ggctcgctca tcgcctttaa caggcgacga aaactgtgct
     7741 gggcatcctg cacgggaagc ataaaagcgg tttccagggt catgcgttgt ctccgcgaac
     7801 catcgtaaag aagtcgaccc ggctggcgtt cacttcggcc tggcgtgcgg caatgcgtgc
     7861 catacggtca gcgtccagcg gggcaataac ggtttctgat aagttttgaa aatagcgaga
     7921 ttgctgcatc agcgcgtcaa tcagcgcgca gcgttcggcg tgctgtttgt cgcgccccag
     7981 cacccagctg tagccgagcg tgccgtcagt caggcgtacg gcggcgcggg tgagcgtggc
     8041 gtcgccggca aaaaagcgtt cgccggtgcc gcccatccgc gcctgaatct gcaccaggcc
     8101 ggtttcggcg gcgcggataa cctcatagtc ggcggtgatg ttcagtgctt tcaggcgggc
     8161 tgccagttca gccggttggc tgtgcgccag tacggacatc cagtgctggc gggtcgcggt
     8221 atctgcgtgc attcagtgct ccatagtgaa ttcaatcatg tcggcgcgcg tcaggctgac
     8281 ggagtactcc gccgggctgc tttcaccgtc acggtggtta agggtgcgca cgcacagcag
     8341 cggcgacata ttcggaattt caagacgctg gcactctttg gcctgggcgc gacgggcgct
     8401 gatccgcgtc tggctgcggc gcagcgcaat tccggtttgc tcgcgcagaa aatcgtgcag
     8461 cgaaccgctg tcgaagcgtt gcagcgtcgg ccaaagggtg aggtccgcaa agtagtggtc
     8521 gattaaacag agcgcgacgc cgttgacgcg acgcagggtg cgcaaatgga taacgttctc
     8581 gccctcggta atccccagcg cgtcagcgac gtgaccggac gcggggcgca ataccgacag
     8641 cagtttttcg ctggtgggat ggctaccctg gtccagcagg ttctggctaa aacgcgcctg
     8701 ggcgttgagc gggtaatcga acgggcgcat cagcaccagc acgccgacgc cctgacggcg
     8761 ctgtacccag cctttttcca ccagttggtc gatggcgcgg cgcagggtgt ggcggttcac
     8821 ctcaaagcgc gccgccagtt gctgctcggc gggaagatag tcgccgcagc ggtagtgttg
     8881 acgaagctcc tgctcaagtt ttgcggctat ctcctgatag cgtgttgggt agctggtcgg
     8941 atgtgtagac aagtgcatag atatcaatgc ctcgcttatc agataaagtg cttacgcaac
     9001 cgttgagaga ggaaatccag caggctgacc gtgacgatga taagcaccat cagggcgcag
     9061 gtttgttgga actggaaacc gcgaatcgct tcccacaggg tgacgccgat cccgcctgca
     9121 ccgaccatgc cgacgacggt cgccgagcgg acgttggatt cgaagcgata gagggagtag
     9181 gagatcagca gcggcatcac ctgcggcagc acgccgtaga ggatctcttc gagcttgttg
     9241 gcgccggtgg cgcgaatgcc ttccaccggg ccgggttcaa tcgcttccac cgcttcggaa
     9301 agcagtttgg agagcacgcc ggtggtgtgg ataaacagcg ccagcacgcc agcgaacggg
     9361 ccgaggccga cggcgaccac gaacagcatg gcgaagacca tttcgttaat ggcgcggcag
     9421 gcgtccatca ggcggcgaac gggctggtaa acccaccacg gcaccaggtt ttcggcgctc
     9481 atcaggccga aggggatgga tagaaccacc gccagcgccg tgccccagac ggcgatttgc
     9541 agcgtgacgg ccatttcggt gaggtaatcc tgccactggc tgaaatcggg cgggaagaag
     9601 tcggcggcga acgtcgccat gttgccgccg tctttaatca gcgtgagcgg ggccatttcc
     9661 gcgccctgcc acgagacgac caacacggcg agaacaacgg cccagctcag aagcgagaac
     9721 cagctacgct tgggtggggc gatggtgatg gtttgcatgt ttggctccgg tctgtaggcc
     9781 ggataaggtg cttgcaccgc atccggcatc aacgcctgca cattgtcgga tgcgacgccg
     9841 gggcgtctta tccgacctac gatttactgc accgctttac tcaccgaact catcgcgctt
     9901 aacgcgttgt tcaggcggtc caggtcatcc agctgcgcct gaatctcggt ggtttttgcc
     9961 agcttgtcct gctcattcag tcctttattg cttttcacgc cctgcatctc tttaaacagc
    10021 gcgagctggc gaatcggcac cagttgcagg tcgctggaag cgcggaacgg cgcccagccc
    10081 aggcgttcca gcaccgcttt ttcttccggc gttttgccgt agttcataaa gaagtcgtag
    10141 atcttgtctt tggtggtttc ggaaaggttt ttacgccaga cgatcggatc gcctgggatc
    10201 agcggcgact tccagatcac cttcagttct ttcagcttct ctggcgcgga ggttttcagc
    10261 ttgtcgaggt tttcggtgtt gttggtggca acatccacct gcttgttggc gacggccagc
    10321 gcgttggttt catgcccagc gttgacggtg cgcttaaagt cgctggcgga gatattgttt
    10381 ttggcaaaga cgtagtaacc ggggacgagg aagccagagg tggagttagg atcgccattg
    10441 ccgaaggtga gatctttccg cttcgccagc agatcgttca ggttgttgat cggactatct
    10501 ttgttgacga tcaacacgct ccagtaacct ggcgatccat ccgccgcgac cgtctgggcg
    10561 aagacctggc cgttggcgcg atccaccgct tccattgccg acaggttgcc gtaccaggcg
    10621 atatccactt tattgaagcg cattccctgg ataatgcctg cgtagtctgg ggcaaagaag
    10681 gcgttcacct tcacgcccag cttcttctcc atatcctgta agaatggcgt ccattgcggt
    10741 ttcaggtttt gctgtgattc cgttgaaata atgccgaaat tcagcgcctt ttcctgctct
    10801 tcggcgtgtg ccgggcttaa cagggtgctg aggctgaaca tgctggtgaa ggccagcgag
    10861 gcaattatct tagcgttcat tcgttttcct caatgatggg gatgtcaggc agctttcgcg
    10921 ttctcttcga tgcgattaat gctgcggtag agatggtcaa aacgttcgtt atcaaactgt
    10981 tggctgctgc cgtcgtagaa aacgtgcccc tggcgcaggg cgacgatgcg ttcgcagtag
    11041 cgcagggcgt aatccacctg atgcagcgtg acgaccacgg tgatgccgtc gttctgattg
    11101 atgtcgcgca gggtgtccat cacgatgcgg gcggattccg ggtccagcga ggcgatgggt
    11161 tcatcggcca gaatcacctt cgcctgctgc atcagcgcgc gggcaatcgc cacacgctgc
    11221 tgctgtccgc cggagagggt ggaaacgcgc tgatgggcaa aatgcaccat gccaacgcgg
    11281 gtcagcgcct gtaacgcgcg ctgtttctgc tcgccggtga accagctaaa acaggtgcgc
    11341 cagaacggcg tgctgccgag cgcgccaatc agcacgttct ccagtacgct caggcggttc
    11401 accaggttga attgttggaa gatgtagccg gtgttggcgc ggcttttgcg gatatcgcgc
    11461 gccagacggc cttcgcgctg gactgtgcgg cccagcagct cgatatggct gccggcggat
    11521 ttatcgccgg taatcaaacc gcttaagtga cgtaaaaggg tggatttgcc ggaacccgac
    11581 ggcccaagca gagccaccat ttcaccgtga tgaatgttca gatcaaccgc atgcagcgcc
    11641 tgatgctgat tgaaggtttt ggcgagcttc tcgacacgga taatcgtttg catgatgcag
    11701 cctccctaaa aaagtggctc catcgtggcg gattattgtg acgatttggt taattaaaag
    11761 tgactaacag atgaagagtt aacgggaatt cgatgacagt gcgggagggc gggcctcccg
    11821 gcgggttatt gcgttggttg ttgtttgacg acattaatca tccacggtac gccaaattta
    11881 tcggtgactt tgccaaagcc atgcgcccag aaagtttcct gccaggccat ttcgattttt
    11941 ccgttagcgg caagattgtc aaaccagcgt tttccttctt cgacctgttg cgaatcgagc
    12001 accagcgtaa agccggagta gctggctttt cctgacggta tagcatcgct catcatgatg
    12061 tcgcttccgg caatgcgcac gttggcgtgg gcgatggcgg tatcgggaaa ttgcattccg
    12121 gaagggcagt tctcggcgct gtcctgcgct gattttggca tttcgccgaa gctgatttta
    12181 tagagcagtt cagcgcccaa cgtacgttga taataagcaa ttgcgtcggc acagttaccg
    12241 gcaaaagaga ggtagggact taacggcatg atggtgacct cagttaagag aagccagtta
    12301 agtgtagttc gaaatttata cagatgagag agaagcctga taagacgcgg tcagcgtcgc
    12361 atcaggcagt cggcatcgtt gccggatgcg gcgcgagcgc cttatccggc ttaccgatca
    12421 caatacaatc agttcttttt cacaaactca gatttcagtt tcatcggacc aaaaccgtcg
    12481 attttgcaat cgatgttatg gtcgccttca accaggcgga tgtttttcac tttggtgcca
    12541 attttcagca tcgaagagct acctttcacc ttcagatctt taatgatggt aacgctgtcg
    12601 ccgtcagcca gcagattgcc gttagcatct ttaacgatca gctcgtcgct ttcctgtgca
    12661 ggttctgcgt cgttccattc gtaggcacat tccgggcaga tgtacatgcc gttatcttcg
    12721 taagtgtatt cggagttgca ttttgggcag tgtggtaatg acatgtggat ttcctcaaaa
    12781 gtcagcaggg caaaagcgcc aaaaaacggc agattgccga aaaaggccgc aattatacac
    12841 aaaatccctg agtttgtcgg gggtactgct gattttagac gacgatcgtt ttcatttgaa
    12901 aggaaacgca aaaagaaaat aatctttttg cctctattta ttatttaaga taatgttaat
    12961 gctctacttg ttcttttttt tattcacatg atatttttat ctttctgacg ttttaaacgt
    13021 atcagaagac gttatttatt ctgataaaaa acaacacact aaatataaaa ttatttcttg
    13081 cagtaattat aaaagccgaa aacagaactc aaaaacaatc tggctaaata aaagtaacaa
    13141 aatttgcttt aaggaagaat tttctatgta cacacagacc ctgtatgagt taagccagga
    13201 ggctgaacgc ctgttacagc tttctcgcca acagttgcag ttactggaaa aaatgcctct
    13261 ctctgtaccc ggagacgacg cgccacaact ggctttaccc tggagtcagc ctaatatcgc
    13321 cgaacgtcac gcgatgctga ataatgagtt gcgtaaaatt tcccgactgg aaatggtgct
    13381 ggcaattgtc ggtaccatga aagcagggaa atcaaccacc attaatgcca ttgttggtac
    13441 ggaggttctg cctaatcgta atcgcccaat gactgcgctg ccgacgctta ttcgccatac
    13501 gcccgggcaa aaggaaccgg tactgcattt ttcacatgtc gcgccaatcg attgtttaat
    13561 tcaacaatta caacagcgcc tgcgtgattg cgatattaag catctgaccg atgtgctgga
    13621 aatagataaa gatatgcgtg cgcttatgca gcggttcgaa aatggcgtcg ctttcgaaaa
    13681 atattatctg ggtgcccagc ctatttttca ttgtctgaaa agtttgaatg atttagtgcg
    13741 actggcgaag gcgctggacg tcgattttcc tttttctgct tacgccgcca ttgagcatat
    13801 tcccgtgatt gaagtggagt ttgtccatct ggcggggctg gagagttatc ccggtcagtt
    13861 gacgttactg gatacccccg ggccaaatga agccgggcaa ccgcatctgc aaaaaatgct
    13921 taaccagcag ctggcacgcg cctcggcggt actggcggtg ctggattata cgcaactgaa
    13981 atcgatctcc gatgaagagg tccgtgaggc gattttggcg gtggggcaat cggtgccgct
    14041 gtatgtgctg gtcaataagt tcgatcaaca ggatcgtaac agtgacgacg ccgaccaggt
    14101 gcgggcactg atttccggga cgctgatgaa aggctgtatt acgccacagc agatatttcc
    14161 ggtgtcgtcg atgtggggct acctggcgaa tcgggcgcgc catgagttag ccaacaacgg
    14221 taagttacca gcgccagagc aacaacgctg ggtggaagat tttgcccatg ccgcgctcgg
    14281 caggcgctgg cgtcatgccg atctggcgga cctcgaacat attcgtcatg ctgccgatca
    14341 gttgtgggaa gattcgctgt tcgcccagcc aattcaggcg ttgcttcatg ccgcttacgc
    14401 taacgcctcg ttgtatgctt tgcgatctgc tgcgcataaa ctgttgaatt acgcgcagca
    14461 ggcgcgggaa tacctggatt ttcgtgcgca cgggttaaac gtcgcttgtg aacaattgcg
    14521 gcaaaatatc catcaggtcg aagaaagttt gcagctattg caactcaatc aggcgcaggt
    14581 gagcggcgag attaaacatg aaatcgagct ggccctgacc tccgccaacc actttctgcg
    14641 tcaacaacaa gatgcgctga atgcgcagtt agccgccttg tttcaggatg attcggagcc
    14701 gttaagcgag atgcgtaccc gctgtgagac actgttacag acggcgcaga acaccatcag
    14761 tcgcgacttt acgctgcgtt ttgccgagct ggaatccacc ctttgccggg tgttaaccga
    14821 tgttattcgc cccattgagc aacaagtcaa aatggaattg agcgagtcag ggtttcgtcc
    14881 tgggtttcat tttcctgttt ttcacggcgt agttccccac ttcaacactc gccagctgtt
    14941 cagtgcagtc atttcgcgcc aggatgcaac ggacgagcag agcacgcgtt taggcgttgt
    15001 gcgtgagact ttttcgcgct ggttgaatca gcccgactgg ggacggggaa atgagaaatc
    15061 cccgacagaa acggttgatt acagtgtgtt gcaacgagca ttaagcgcag aagtcgatct
    15121 ttattgccaa caaatggcta aagttctggc agagcaggtc gatgaatctg ttacggcagg
    15181 catgaatact tttttcgctg agttcgcttc atgtttgacg gaattacaga cgcgtttacg
    15241 cgaaagcctg gctctgcgtc aacaaaatga atcggtggtc aggctgatgc agcagcaatt
    15301 gcagcagact gtgatgactc acggctggat ttacaccgac gcccagctgt tacgcgatga
    15361 tattcaaaca cttttcacgg cagaacgata ttgaccaaga cgttactgga cggccccggt
    15421 cgcgtgctgg agtcggttta tccccgcttt ttagtggatc tggcgcaggg cgatgatgcc
    15481 cgccttccac aagcccatca gcagcagttt cgtgaacgac tgatgcagga acttctttcg
    15541 cgtgtgcagc ttcagacatg gacgaacggc ggcatgttaa atgcgccgct tagcctgcga
    15601 ctgacaatgg tggaaaaact ggcgtcgatg ctggatcccg gtcatctggc gctgacgcag
    15661 atcgcgcagc atctggcgct gctgcaaaaa atggatcacc gccagcactc tgctttcccg
    15721 gagctccccc agcaaattgc cgccttgtat gagtggtttt cagcccgttg tcgctggaag
    15781 gaaaaggcat taacgcaacg aggcctactg gtgcaggcgg gtgaacagag cgagcaaatt
    15841 tttacccgct ggcgtgctgg ggcgtataac gcctggtcgt tgcctgggcg ctgttttatc
    15901 gttctcgaag agttgcgttg gggggtattt ggcgatgcct gtcgtctggg aagcccgcaa
    15961 gcggtggcgt tgttgctggg tgatttgcgc gtgaaagcga cacaacatct ggcagagagt
    16021 atcaatgcgg ccccgaccac gcgtcactat taccatcagt ggtttgcctc ttcgaccgtt
    16081 ccgacgggcg gggatcatgc tgatttttta agttggctgg gaaagtggac cacggcagat
    16141 aaacaacccg tttgctggtc agtgacccaa cgctggcaaa ctgtcgcgct ggggatgcca
    16201 cgactctgtt cagcgcagcg tctggcgggg gcaatggtcg aggaaatctt ctctgtaaat
    16261 ttggcgtaaa taatcagtta catcaatgag tcctaaacga aatccatgtg tgaagttgat
    16321 cacaaattta aacactggta gggtaaaaag gtcattaact gcccaattca ggcgtcaact
    16381 ggtttgattg tacattcctt aaccggaggg tgtaagcaaa cccgctacgc ttgttacaga
    16441 gattgcatcc tgcaattccc gctccccttt tgcggccgtc gcgctgattt ttctggcgtt
    16501 tgcggaaatg ggccaactct gcgaggaaag ctatgctgaa aaggaaaaaa gtaaaaccga
    16561 ttacccttcg tgatgtcacc attattgatg acggtaaact gcgtaaagcc attaccgcag
    16621 catcactggg taatgcaatg gaatggtttg attttggtgt ttatggtttt gttgcttacg
    16681 cattaggtaa agtttttttc ccgggggctg accccagcgt gcagatggtt gctgcacttg
    16741 ccacgttctc cgttcccttt ctgattcgac cgcttggcgg gctcttcttt ggtatgttgg
    16801 gcgataaata tggtcgccag aagatcctcg ctatcactat tgtgattatg tcgatcagta
    16861 cgttctgtat tggcttaata ccgtcctacg acacgattgg tatttgggca ccgattctgc
    16921 tgttgatctg taagatggca caaggtttct cggtcggcgg tgaatatacc ggggcgtcga
    16981 tatttgttgc ggaatactca cctgaccgta aacgtggctt tatgggcagc tggctggact
    17041 tcggttctat tgccgggttt gtgctgggtg ctggcgtagt ggtgttaatt tcgaccattg
    17101 tcggcgaagc gaacttcctc gactggggct ggcgtattcc gttctttatt gctctgccgt
    17161 tagggattat cgggctttac ctgcgccatg cgctggaaga aactccggcg ttccagcagc
    17221 atgtcgataa actggaacag ggcgaccgcg aaggtttgca ggatggcccg aaagtctcgt
    17281 ttaaagagat tgccaccaaa cactggcgca gcctgttgac atgtattggt ctggtaattg
    17341 ccaccaacgt gacttactac atgttgctga cctatatgcc gagttatttg tcgcataacc
    17401 tgcattactc cgaagaccac ggggtgctga ttattatcgc cattatgatc ggtatgctgt
    17461 ttgtccagcc ggtgatgggc ttgctgagtg accgttttgg ccgtcgtccg tttgtgctac
    17521 ttggtagtgt tgccctgttt gtgttggcga tcccggcgtt tattctgatt aacagtaacg
    17581 tcatcggcct gatttttgcc gggttactga tgctggcggt gatccttaac tgctttacgg
    17641 gcgttatggc ttctaccttg ccagcgatgt tcccgacgca tatccgttac agcgcgctgg
    17701 cggcggcatt taatatttcg gtgctggttg ccggtctgac gccaacactg gcggcctggc
    17761 tggtcgaaag ctcgcagaat ctgatgatgc ctgcctatta cctgatggta gtggcggtga
    17821 ttggtttaat caccggcgta accatgaaag agacggcaaa tcgtccgttg aaaggtgcga
    17881 caccggcggc gtcagatata caggaagcga aggaaattct cgtcgagcat tacgataata
    17941 tcgagcagaa aatcgataat attgaccacg agattgccga tttgcaggcg aaacgtaccc
    18001 gcctggtgca gcaacatccg cgaattgatg aataagctga aacggatggc ctgatgtgac
    18061 gctgtcttat caggccaatt gaactcttaa ggttcactta atctctgacg cgcatactct
    18121 cctccaggtt aacggaggag agtgcaatga aaaaccgtgt ttatgaaagt ttaactaccg
    18181 tgttcagcgt gctggtggtc agcagctttc tttatatctg gtttgcctcg ttctgatctt
    18241 tcttcagccg tacccaggcc cgcgtgccgg aagtctcttg ccggttttgc aggaaaaact
    18301 gcccgtgatg caactgtgta atgcggctga caatacttaa ccccagacca atcccgccat
    18361 aacggctgtc catacgtaca aacgctttac tcaactcccc gcatttactc tcatcaatac
    18421 ctggtccttc atcttcaact gccatgaccg ctccgccatc ttcttgcagc ttaatcataa
    18481 tgttgctgcc ttgcgggctg taacgatggg cgttttctac caggtttcgc aataacatcc
    18541 gcagcagggt tgcatcaccc tgaacggtga tgtcggcggc gctctctggc aatagcaggg
    18601 tttgctgtcg ctggtcgagc atggtactga gttcgtcata cgaggggaga atgacatctt
    18661 ccagcagttt tacatgttga taattaccgg aagaaaatga ctgtccggca cgcgccagtt
    18721 gcagcagctg ggagacgctc tccatcatct gatcaagccg tgccactaac ggtgctacat
    18781 caatgtgatg cgttttcgcc agcagttcca gatgcaaacg cacccccgcc agtggcgttc
    18841 gcagttcgtg cgcgacgtca gcggtaaaca acctttcgtt atccagcgtg ctggtcaggc
    18901 gactgaccag atcgttcaac gccgaaacca ccgcttcgat ttcgagggtg gcgctgtgaa
    18961 tggcaatggg cgttaagttg tcggcggtgc gcgcttccag ctctttttgc agctccgcca
    19021 gcgggcgagt gatgcggcgt accgcctgat agcagataaa tagcgtcagg ctgaccataa
    19081 agacgccggg gacaatcagg ctggcgaccg cctcgcggat ctcacgcatg atgtggcgat
    19141 cgttgttgcg attgtcgcgt agcgcctgct caaacagctg aatctgctcg gtactttcat
    19201 gccatagcca gaagacgctg atcagctcaa acaccaacaa aatggccccg atggtcaata
    19261 tcagccgttg gcgcagcgat attggtcggc gcagaaaatg catcagattc aattagtttt
    19321 cctcattcgc gaccagcata tagccaaagc cgcgcacggt gcggatacgg gctttgccca
    19381 ctttgtcgcg cagattgtgg atatgcactt ccagggtgtt ggtcgagggt tcattgtccc
    19441 agttatagat gtcgttgtag agaatttccc gatgcaccgg actgcctgct ttgagcatta
    19501 accgtgacag cagagcatat tctttgggcg tcagaatcaa ctcttcaccg cccatccata
    19561 cctgacggcg acccatattc agcgtcagat tgccaacaat cagctcactt tcgccctgat
    19621 tattatggcg tcgtagcagg gcgcggatac gggcgtgtaa ctcttccagc gcaaaaggct
    19681 tcaccagata gtcatcggca ccgacatcca gcccagcgat tttgtcggtc agcgtatcgc
    19741 gagcggtgag gatcagtact ggcagggtgt attttttctg ccggatacgg gcgagaaaat
    19801 gcagtccatc ttcgtcgggt aaccctaaat ccagtaccac caggctgtaa tgaccggcct
    19861 caaggctttg ttccgccatc cgcgcggttg tcacgccatc gcacgcgtag ccttcagttt
    19921 gcgccgccag aatcaatccc tgcaataaca gcgtatcgtc ttcaacaatc agaattttca
    19981 ttcactcact ctcctgcaag tttgcagaat atcatccgca gcctggtaat acttcgtctc
    20041 aacgccagtt aatcccaata gcgtggagaa taaattgtct tgtgaatagt gttgcgtttg
    20101 cgcctgtttt tgcaggcagt tctggtcaac ctgataccgt ttttgataat cctccgacag
    20161 ccacagcagc atcggcacct gtttttggct atccggggcg atggcataag gcagaccgtg
    20221 cagatagatg ccattttcac ctaacgattc accgtggtca gaaagataaa ccaggctggt
    20281 ggtaaattta tcctgatgtt ctttcagcag attaatcgct ttatcaacaa tatagtcgac
    20341 gtaaaccagc gtgttgtcgt aagtgttcac cagttgctct ttgctacagg tctggatctc
    20401 attggtgtcg caggttgggg taaatttcct gaactgcggc ggatagcggt tgtaataggt
    20461 cggaccgtgg ctgccgatgg tgtgtaagac aatcacgcca tcaccctgca ggttattgat
    20521 gtactcttcc agcccgtgga acagcacttc gtcatagcat tcgccgttga tgcactgacc
    20581 aggcagattc agcgcggtga cgttctggtg aggcacgcgg tcgcaggcac ctttacagcc
    20641 gccatcgttg tcattccaca gcacgttgat gcccgctcgc tgaatgatat ccagcacgcc
    20701 ttcctggtgc tgtgccagct cttctttgta atgctcacgc ggcatatccg agaacatgca
    20761 cggtactgaa actgccgttg ccgtgccgca agatgcggta ttagggaaat agaccacgtt
    20821 atctttcgcc agccgcgggt tagtttcacg cgggtagccg ttgagggaga agttctccgc
    20881 ccgcgaggtt tcgccgacaa tcaggatggt caaattttta cgtttttcgt tctgcattaa
    20941 cgggttgcgg tgggcgtctt caccaattcg caccagcggt agatttgcca gtcgctgatg
    21001 ggagtaccat gaccagctgg caacaatgct gttagagggg cttaaggatt tcaccagctc
    21061 tttgttattg cggaacaacg aggcgtagtc tttataaaac agtgcggcga ccagcaaaat
    21121 cagtagtaca gaaaccagaa tattggctcc acggaggaaa agaacactgc gcagacgcga
    21181 ggtggcaggt ttgattttta tccagcaggc aatcagcgca gcaagcacgc cgctgaatcc
    21241 cagcgttaat aacatttgcg gtgtcatcag cgcataactt tctgccggag tggtatcaat
    21301 aatattggca atcatcgagc ggtcgatgac gatgccgtaa gtcattatga aatattgtgc
    21361 agccgcgcca accagaataa acaggcaggc cagtggtcga ttaagccata agaaagagct
    21421 tagtgtcagg acaatattaa tcacgctgaa agcgacgacc ggcatcgaca agaaaaccag
    21481 tacgttatgc agcgaatcca gcggcagcgc ctgcaacacc tgtttaaaaa aggcaatatt
    21541 caggcagata gagatataaa aagcggccaa caatagccag gcgagtaaat tcaaagaggg
    21601 tctttttagt aggcgcttca acatcacggt gtttccatcg aacaaagtgc gcatatgctc
    21661 gcaaagcaaa attaagccaa ccttaagttc ttaaggttgg cttttatgtt tgctggatta
    21721 aggggaatta atctttgctt attggtgcat ctaagggata cgggttttta tgcagccggt
    21781 tgtaattcag cgcatacatg gcggtgatta ccatcagggt gacaaatgac cacataacct
    21841 ctttcgcccc ggaccccacc acggcccaaa tgcagtagag gaaggcaatg gtagtaacag
    21901 ccagatatgc cgggcgtgct ttaccaaagt gaccgtgacc gagcagcagt aacgccgcac
    21961 aggtgtaaag atatggcacc agtgtaaaga tgaccgagac ggaagaaacc agaccgaact
    22021 ctttggtcgc gtttggtgaa atgctgctga gctggaagat ggtcatcaaa ataccgacga
    22081 taatcagccc cgccactggc gtgcccgctt tatttacacg ggcaaaaatc ggtgggaaca
    22141 gtccgtcatc ggcagcggct ttcgccgttt gacccgccag caacgtccag ccgcccagtg
    22201 aacctaagca acccgcagct gcgcagaagg aaacaatggc cccggcggtg tcacccaacg
    22261 ccatccgtgc ggcatcaccg aatggcgaag cagaaacgcg cagtgcggca ttagggatca
    22321 tccccataat cgcggtggta gaaagtacat agcaaacggc ggcaatcaat accccaccaa
    22381 tggtggcgat agggacattg cgtttcgggt ttttcaccac acctgcggca acggaggcac
    22441 tttccacacc gatgaacgac cacagcgtaa cgttaagggt actttgaatt gcaccgaagg
    22501 tgcccaggcc gctgacgttc catgccgcca tataggtttc accacggaac cagaaccagc
    22561 caaatacggc aatcccgacg atggggatca gcgccagcac ggtggcaact gcctgcacac
    22621 gggtgatcat tttcggaccg acaatgttca gcaggacgaa gatccacagc accacgacgc
    22681 aggtgatggt taataccaat ggatctttca gaatcgggaa gaagtaactt aaatatccta
    22741 cgccaatgac caccatggcg atattgccga tccagcaggc cagccagtag aggacgttgg
    22801 tttgataacc gagaaacggg ccaaagcagc ggcgggcgta agcgtaagaa ccaccaggac
    22861 ttggatcgag gaacgacatt ttggcgtata ccatcgagag ccccagcgca ccgataatcg
    22921 tcaccaacca tccataaatg gcaatcccgc cagtggaggc caggtttgca ggtagcagaa
    22981 aaacacctga ccccataata ttccccgaca ccatcagggt gacggggatt aagcccactt
    23041 tgtgagcatc agcatccgaa gacataatta aactcctgcg aaggcgagct tcgtgacaat
    23101 aaattacgtc atatcatacg cctgcattcg atagttgaat tattatcgac cgggttatcg
    23161 ctgatgccgt tattaatgta gctcttcgtt aaggttaaaa atcaggcgac agtgcgttct
    23221 ctgtgctgac taacataatg cagcggcgtc ataccgtaga agtctttaaa gacagaaata
    23281 aagtacgacg tactgttgta gccgcaggac tgtgatacct gagagatatt tttaccgtcc
    23341 atcattaatt catttacggc ataacgcatg cggcaggtgg tgattatttg gctataactg
    23401 gtgttttcgc ttttcaactt tttctttaac agacttgggc taagacataa acaactggct
    23461 accatactaa gattccagtc tttgtgaata tcgctttcaa taatttgata aacgctgtca
    23521 cttacacaat tacgtaacat atacatcatt aatgaaagga attttttact gtcgagaaaa
    23581 cgggatagca cagtgaataa taatgcgcgt gttcgttcct tttctgactc agtttctgcg
    23641 ggcatcatgc tgtgttgcgc ggcaacgcga aagacgtctg gcgtcaggca tggcagggtg
    23701 agtatgggcg tagcgctacg ttgccatact ggtatttgcg agagatcttt attcagaaac
    23761 cggaggtaat ctttgatgat gtcgtgacta atatgagcaa ccaaagtgtt gttaagagag
    23821 gagacgtcga taatattatt ttcacaattt aataacgcca tatggttagc tttaaggcta
    23881 ataggttctt tcccattcac ccttatccag acatcttttt cagtcaataa aacaatacaa
    23941 ggttggtcgc tgcaaatcct catacatgta ctcctgagtg cgaataaaag accagttctc
    24001 gctaaagcaa agcgataccg ggaaatggtt atataattgt atgatgaata taaacatgca
    24061 taaaaataac gagttttact aatattgcaa atttttatag taatttctca tgtacatatt
    24121 tttttacagg ataaataaca ggcaaaaaaa agcgcggggt ggggccgcgc aaaaagtatg
    24181 acatttttgt ttgcagcaat ttttatgaaa acgttgccat actcaaaagg cctaaggcca
    24241 ggcggcgtaa tgttatttaa acaattacgc cttcagcgga atagtggtta cgctttcacg
    24301 cacataacgt ggtaaatacc gtcaataatt tcagtccctt cagtttcgtg ttcaaatcca
    24361 gggaaatggt ggtcccagga ttgcagcgag cgtaaataac ttacttgcgg actgttttta
    24421 tcgccgaagt tttcaccaga cagcagcatc gggattcctg gcggatacgg gataactgag
    24481 tttgccgcga tgcgtcctgg cagattttca atggatacca gttcgacatt gttgtcgaca
    24541 atcgcgttgt acgcttcacg cggggtgact tccgccaccg gcaggccgga ataggcttcg
    24601 ttcaaccgtg cgccagggtt gttttctttc agccaggcaa acatggtgtc acccagatcg
    24661 tgaatcccca tgttcgcgta agtgtcagga tattgttcaa caagttccgg catcacctgc
    24721 gccagcggtg tgttggcgtc atagtggcgt ttgaaggagc aaagggtgtt aaccagagtt
    24781 ccccatttcc cacgggttac gcccatagag aacaggaaca taatttggaa gtcagtggtg
    24841 cgggtaggta caatgccgtg gcgaccaagc caggcagtga ccagcgccgc cggaacaccg
    24901 gtttcttcca gttcaccatc ttcacccatt cccggagcaa ggatgctgac tttaatcggg
    24961 tcgagcatac tccagttatc cggaatatct ttgaagccgt gccagctttc gcccggatgc
    25021 attacccagc agtcctgaac ggtggtcagc agtttggttg gtgcgtcagc aaagtcatag
    25081 gttttgccgg tttgtgggtc ggtgacgact tctttgttcc acggtttgaa gaaccagcta
    25141 ccgtcagcgg tgaactcttt atacagccgc gccatcgcct gacgaaaatc aaccgcttcg
    25201 tcaatcactt cttgtgtcag tgacaggccg ctgttgccgt ccatcatcga caccgccacg
    25261 tcgttggatg cgcagatggc atacagcggg gaggtggtgg catgcatcat gtaggcctgg
    25321 ttgaagcggg agaagttaat cgccccacga ccttcacgta catgaatata agaagcctgt
    25381 gacagcgcat tcagcagttt gtgggtggag tgggtggcga aaacggtagg accgttgtga
    25441 tcgccaggtt cgccgcgcat ggcatagtga tcggcataga tcgggttgaa acgtgcatag
    25501 ccgtaccagg cttcgtcaaa gtgcagacga tcggaggttt tttccagcag atcctgcgct
    25561 tctttagcgt tataacacac gccgtcatag gtgcagttgg tcaccacgca gtaagacggt
    25621 ttttgcccag ctttgtcttt agtcagcggg ctttcactga ttttcttctg caaggtttca
    25681 ggttgcattt cctgcggata gattggcccg ataatgccgt agcggttgcg gcttggcacc
    25741 atgtagactg gtttcgcgcc tgtcagcatc aagccttgtt cgatggattt atggcaatta
    25801 cggtcaacga ccacgacatc gttatcggtc atgcaagcct gcatgatggt gcggttagag
    25861 ccggaagtac cgacgactac cgaccaggag cgatcggcac caaatacgcg tgcggcatat
    25921 ttttcgcttt cgccaaatgc gccagtatgg tcaagcaaag aaccgaggga agttcgttcg
    25981 atgcccatgt cggtgcgaaa cagattttca ccatagtagt catggtagaa acgtccggcg
    26041 ggtgttttgg taaaaccaac gccgccctgg tggcctggcg ctgcccagga atattcatgg
    26101 atgtcactat atttcatcag cgcgctgaac agtggcggca acagctgctg gcggtagcgg
    26161 gtcatcgcgg caacggcgcg tccggcgata aagtcggcgg tatcttccag aatccaggcg
    26221 aattcatcga caagctccag cagatcccgg tccattgcgg cgagggcttt ttcccgatcg
    26281 cccaacagga agaccggcac gttttgttgg cgctcatgaa gcttaccgat caattgtctg
    26341 acgttttgat gttcgtccgg atgttccatt tgatagctga acatcaggca gtcaatggct
    26401 tcgtttgaag agagaatggc aaaaccatca tcaaaggagg tggatttaat cacggtaaca
    26461 ttttgctggc ttaaagcatc tgccagacgc tcaacggcgt taccgaccca ggtgtcttga
    26521 tggagaaact cgctttcaac aattaatact ttcatcattg cttacccggt tatgaaggaa
    26581 tcttcgttgc gggcaagtat cttccggcct tttcgggctg taaagcgcgt gaaaaacaaa
    26641 catttttgaa atatttagtt ttataaacaa tacgttatgt gattatttta aattattttc
    26701 actgcttatt atcgacggct aaactatttt tttggctgat actgatatcg tctttagccg
    26761 ggaaacgtct ggcggcgctg ttggctaagt ttgcggtatt gttgcggcga catgccgaca
    26821 tatttgccga acgtgctgta aaaacgacta cttgaacgaa agcctgccgt cagggcaata
    26881 tcgagaatac ttttatcggt atcgctcagt aacgcgcgaa cgtggttgat gcgcatcgcg
    26941 gtaatgtact gtttcatcgt caattgcatg acccgctgga atatccccat tgcatagttg
    27001 gcgttaagtt tgacgtgctc agccacatcg ttgatggtca gcgcctgatc atagttttcg
    27061 gcaataaagc ccagcatctg gctaacataa aattgcgcat ggcgcgagac gctgtttttg
    27121 tgtgtgcgcg aggttttatt gaccagaatc ggttcccagc cagagaggct aaatcgcttg
    27181 agcatcaggc caatttcatc aatggcgagc tggcgaattt gctcgttcgg actgtttaat
    27241 tcctgctgcc agcggcgcac ttcaaacggg ctaagttgct gtgtggccag tgatttgatc
    27301 accatgccgt gagtgacgtg gttaatcagg tctttatcca gcggccagga gagaaacaga
    27361 tgcatcggca gattaaaaat cgccatgctc tgacaggttc cggtatctgt tagttggtgc
    27421 ggtgtacagg cccagaacag cgtgatatga ccctgattga tattcacttt ttcattgttg
    27481 atcaggtatt ccacatcgcc atcgaaaggc acattcactt cgacctgacc atgccagtgg
    27541 ctggtgggca tgatatgcgg tgcgcgaaac tcaatctcca tccgctggta ttccgaatac
    27601 agcgacagcg ggctgcgggt ctgtttttcg tcgctgctgc acataaacgt atctgtattc
    27661 atggatggct ctctttcctg gaatatcaga attatggcag gagtgaggga ggatgactgc
    27721 gagtgggagc acggttttca ccctcttccc agagaggcga ggggagcgtc cgagcatcat
    27781 gaggccgaaa actctgcttt tcaggtaaat tattaccata aactcagatt tactgctgct
    27841 tcacgcagga tctgagttta tgggaatgct caacttggaa gccggaggtt ttctgcagat
    27901 tcgcctgcca tgatgaagtt attcaagcaa gccaggagat ctgcatgatg actgcaccca
    27961 aaattacatt tatcggcgct ggttcgacga ttttcgttaa aaatattctt ggtgatgtgt
    28021 tccatcgcga ggcgctgaaa acggcgcata ttgccctgat ggacattgat cccacccgcc
    28081 tggaagagtc gcatattgtg gtgcgtaagc tgatggattc agcaggggcc agcggcaaaa
    28141 tcacctgcca cacccaacag aaagaagcct tagaggatgc cgattttgtc gtggtggcat
    28201 ttcagattgg cggttatgaa ccttgcacgg tgactgattt cgaggtctgt aagcggcatg
    28261 gtctggaaca aaccattgcc gatacgttgg ggccgggcgg tattatgcgc gcgctacgta
    28321 ccattccgca tctgtggcaa atttgcgagg acatgacgga agtctgcccc gatgccacca
    28381 tgctcaacta tgttaacccc atggcgatga atacctgggc gatgtatgcc cgttatccgc
    28441 atatcaaaca ggtcgggctg tgccattcgg tgcagggaac ggcggaagag ctggcgcgtg
    28501 atctcaatat cgacccagct acgctgcgtt accgctgcgc aggtatcaac catatggcgt
    28561 tttacctgga gctggagcgc aaaaccgccg acggcagtta cgtgaatctc tacccggaac
    28621 tgctggcggc ttatgaagca gggcaggcac cgaagccaaa tattcatggt aatactcgct
    28681 gccagaatat tgtgcgctat gaaatgttca aaaagctggg ctacttcgtc acggaatcgt
    28741 cagaacattt tgccgaatat acgccgtggt ttattaagcc aggtcgtgag gatttgattg
    28801 agcgttataa agtaccgctg gatgagtacc cgaaacgctg cgtcgagcag ctggcgaact
    28861 ggcataaaga gctggaggag tataaaaacg cctcccggat tgatattaaa ccgtcacggg
    28921 aatatgccag cacaatcatg aacgctatct ggactggcga gccgagtgtg atttacggca
    28981 acgtccgtaa cgatggtttg attgataacc tgccacaagg atgttgcgtg gaagtagcct
    29041 gtctggttga tgctaatggc attcagccaa ccaaagtcgg tacgctacct tcgcatctgg
    29101 ccgccctgat gcaaaccaac atcaacgtac agacgctgct gaccgaagct attcttacgg
    29161 aaaatcgcga ccgtgtttac cacgccgcga tgatggaccc gcatactgcc gccgtgctgg
    29221 gcattgacga aatatatgct cttgttgacg acctgattgc cgcccacggc gactggctgc
    29281 caggctggtt gcaccgttaa aacgcgacta aacgctactg cgccggggga tttattccgg
    29341 cgcacacctc tgacgatacc aataacagaa ggcgggcgtt ggtaacagcg acccgatacc
    29401 ctatgagcat ttcaatgact acaaaactca gttatggatt tggagcgttc gggaaggatt
    29461 ttgcgatcgg cattgtgtat atgtacctca tgtattacta caccgatgtc gtcgggctgt
    29521 ctgtgggttt ggtcggtact ttgtttctgg tggcgaggat ctgggatgct attaacgatc
    29581 cgattatggg atggattgta aatgctacgc gatcgcgatg gggtaagttc aaaccctgga
    29641 tcctgatcgg tacgttggca aactctgtaa tcttatttct cctctttagt gcgcatctgt
    29701 ttgaaggtac tactcagatt gtctttgttt gcgtgaccta catcctctgg ggcatgactt
    29761 acaccattat ggatattccc ttctggtcgc tggttccaac catcacgctc gataaacgtg
    29821 agcgcgaaca actggttcct tatccgcgtt tttttgccag tctggcaggc tttgttacgg
    29881 caggtgtgac gctaccattt gttaattatg tcggcggtgg cgatcgggga tttggctttc
    29941 agatgttcac tctggtactg atcgcctttt ttattgtttc aaccatcatc actctgcgca
    30001 atgtgcatga agtcttttcg tcagacaatc aaccgtctgc tgaaggaagc catctgacac
    30061 ttaaagccat cgttgcgcta atttataaaa acgatcagct ttcatgcctc ttgggtatgg
    30121 ctcttgctta taatgtagcc agcaacatta ttaccggatt tgctatctat tatttctcat
    30181 atgttatcgg tgatgcggat ttgttcccct attatctgtc gtatgcggga gctgctaacc
    30241 tggtgacgtt agtattcttc ccacgcttag ttaaatcatt atcccgacgc attttatggg
    30301 ccggagcatc tattcttccg gtgttaagct gtggtgttct cctgttaatg gcattaatga
    30361 gctatcacaa cgtcgtcctc attgtgattg cgggtatttt gctgaatgtg ggaacggcgc
    30421 ttttctgggt attacaggtc atcatggtgg cagataccgt tgattacggt gaatataaac
    30481 tgcacgtacg ctgtgaaagt atcgcttact ccgtgcagac tatggtggtg aagggcggtt
    30541 cagcctttgc ggcttttttc attgcggttg tgttagggat gattggctat gtaccgaatg
    30601 ttgaacagtc tacgcaagcc ctattaggta tgcagtttat tatgattgct ctaccaactc
    30661 tgtttttcat ggtaacgctg attctctact tccgtttcta tcgcctcaat ggcgacacgc
    30721 tgcgcaggat ccagattcat ctgctggata aatatcgcaa agtaccgccc gagcctgttc
    30781 atgctgatat tccggtcggt gcagtgagtg atgtgaaagc ctgacgtgga caatagctaa
    30841 cgaaaatggc ctgatgcgat atgtttatca ggccacgtct taatgtaatg ctttggattt
    30901 gcaaattttg tagaccggat aaggaattca cgccgcatcc ggcatcaaca aagcgcacgt
    30961 tgttatccgg ttatcaagcc aaagcgccgt agctggcggc aatgaaagcg agcgaggaat
    31021 atcaccgtcg ttaatgcgcc aagcagcgcg cagaacatat cggattgcgt gtcccactgg
    31081 tcgccctggg tgccgagaaa atcatccgct ccctggccca ttgccagcgc cgcccaccac
    31141 tcgattaatt catacatcgc gcttatcgcc agcgccacgc agcagaccag gaacgccacc
    31201 attttacgtc cgcgcacgta catcccgcga acgagaattt ctcgtgccac cagtgcaggc
    31261 accagcccct ggaaaaaatg ccccagcttg tcatacggat tacggctcaa ccctaaccat
    31321 tcctgcacct caaaaccaac ggggactttc gcgtaggtgt attgtccgcc gaccatcagg
    31381 atgatggcgt gaaagaaaat gagcgtatag agcagcggcg ttaacggata acgtttcgcg
    31441 gtggcaagga gtagcggcac gacaataatc accggtgtca cttccatcag ccaggtgagt
    31501 ttgtcatggg ccgaaatgcc ggtataaatc aggattaacg ttagcgccag tgcgccggtg
    31561 ttaagaatta acggcttgag tgtgcgggtc atggtgagtt cgcaagtcag ggaaaatact
    31621 gactattcac cagcaagcgt gaaattacaa tcgcaaaaga ggtggcccag ggggatcacc
    31681 tggcagcatg ctgccaggcg ctgggccgaa gaggttactt agtgcagttc gcgcactgtt
    31741 tgttgacgat ctgctggaag aagtcgttac ctttgtcatc gaccaggata aacgccggga
    31801 aatcttctac ttcgattttc cagatagctt ccatacccag ctccggataa gcgacgcatt
    31861 ccagatgctt gatgctctgc tgcgccagta ccgccgccgg accgccgatg ctaccgaggt
    31921 agaagccgcc gtgtttatga cacgcgtcgg taacctgctg actgcggtta cctttcgcca
    31981 gcatgatcat gctgccgccg tgggattgca gcagatccac gtaggagtcc atacggcctg
    32041 cggtggttgg gccaagtgaa cctgatggat aaccggcagg ggttttcgcc ggacccgcgt
    32101 agtagatcgg gtgatctttg atgtactgcg gaagttcttt accggcgtca atcagctctt
    32161 tcagcttggc gtgtgcaata tctcggccca cgataatggt gccggtgagc gacaaacgag
    32221 tggataccgg gtattgcgaa agctgggcga ggatctcttt catcgggcgg ttaaggtcaa
    32281 ctttcaccgc ttcgccttca ccggcctggc gcagttcttg tggaatgtac tggcctgggt
    32341 tgtgttccag tttttcgatc cagatacctt tgcggttgat tttcgcttta atgttacggt
    32401 cagcggagca ggagacgccc atgccgaccg ggcaggatgc gccgtgacgt ggcagacgga
    32461 taacgcgaat gtcgtgcgcg aagtatttac cgccaaactg cgcgccaaga ccgagtttct
    32521 gggcctcttc cagcagttcc tgttccagct ggacatcgcg gaacgcctga ccatgttcgt
    32581 tcccttccgt cggcagttca tcgtaatagt gagcgcttgc taacttgacg gttttcaggt
    32641 tggtttccgc agacgtaccg ccaatcacaa acgcgatatg gtacggcggg caggctgcag
    32701 taccgagggt acgcattttc tcgacgagga agtttttcag tttgccggga gtcagcaggg
    32761 ctttggtttc ctggtagaga tacgttttgt tggcagagcc gccgcctttc gcaacgcaaa
    32821 ggaatttgta ctcatcgcca tctaccgcgt acaggtcgat ttgcgcaggc aggttagtgc
    32881 cggtgttgac ctctttgtac atgtccagcg ccgcattctg tgaatagcgc aggttatctt
    32941 cgatataggt gttatagacg cctttcgaca gcgcttcttc atcaccgccg ccggtccaca
    33001 cgcgctggcc ttttttaccg acgatgatcg cggtgccggt atcctggcag gtcggcagca
    33061 cgcctttggc ggcgatttcg gagtttctta agaattgcag cgccacgtac ttgtcgtttt
    33121 cgctggcttc tggatcgtga agaatagccg caacctgttt ctggtgtgcc gggcggagca
    33181 taaaagaagc gtcgtgaaag gcttgctgcg ccagcagggt cagggcttct ggttccactt
    33241 tcaggatggt ttcgccgtcg aagtcggcaa cgctaacgta atcggaagtg agtagatagt
    33301 attcggtatt gtctttcccc atcgggaaag gtgcctggta gataaagggt ttgtttgaca
    33361 tagcttccag cctgtaactc tgtatttgtt attcgaaaat ggcgtgccgc gtagtcacgg
    33421 gcggcacgca aagtgcattt ataagaaccc gtacatcgcg gcgaagatcc agccgaagac
    33481 gcacgatacg ctcacaccaa tcaaccccgg cagaataaag ctgtggttga tgacgaagcg
    33541 accgatgtgg gtggtgccgg aacggtcaaa ctgaatcgct gccagatcgc tcggataagt
    33601 cggcaggatg taataaccgt agcaagccgg tgctgaagcc acgatgtatg ccggatcaac
    33661 gccgatcgcc agcgcgaccg gaacaatcgc cgccagcgcc gcagcctgag agtttacaaa
    33721 cttggaaacc agcagcagaa caatggcata ggcccacgga tactctttca ccatttcacc
    33781 cagtacgccc tgaatttcag acatatgcgc accgaacatg gtttctgcca tccatgcgat
    33841 accgtacacc gccacgatgg cgatcatacc ggaacggaag acttcgtttt ttgagataga
    33901 cgcgggattg gttttggtca ggataataat cagcgccccg gtcagcagca taaacatctg
    33961 aataaccagt accatcgaca gcggtttgcc gccgaaggat ggacgcaggt ccgaatcagc
    34021 accaagaagg gcgactacag cgattgcacc gaggaaaatc cacattgcca gccagttgct
    34081 tttcggcagt tttttatcga gcagcgtcgc ggtatcaccg taaacatact cacggttttc
    34141 cggtacggag atgaatttct ggaactcttc gtctttatcc agatctttac cgcggaacca
    34201 gctgaagata ccgatcgcca ggataccgat taacgtcgat ggaatggtga ttgccagcag
    34261 gtcgaggaac tcaagatggc gaccatcaaa ggtgacatta cccagcattg caaccagaga
    34321 cacgaccgca accgacaccg gactggcgat aatccccatc tgtgcaccga tagaacttgc
    34381 cgccatcgga cgttccggac ggatgttgtt cttaatggcg acgtcgtaga tgatcggcag
    34441 aatggtgtaa accacatgac ccgtaccgca aagaatggtc agtgtacagg tcacaaacgg
    34501 cgcgacaatt gagacatatt tcgggttgcg gcgcagcaac ttctcggcaa tttgcagcat
    34561 gacatcaaga ccgcccgaag cttgcaaggt cgccgatgcc gccaccaccg caatgataac
    34621 cagcatgaca tcaactggtg gtttacctgg ctgaaggtgg aagacgaaga ccagaatgac
    34681 cagaccgata ccgcctaata aacccagcgc gataccaccc tttctggcac cataaaacag
    34741 acatatcagt attatgataa gttggatagt aaataacatg tgtgaaccct cgcgataatc
    34801 ctatttaaat ttttgctgaa tagatcacag tcacgttctg ttttgtatga actgttttca
    34861 gagcctgact aaatatccgt ttggtcgtta ccggctttag caaatgcctc acagtgaata
    34921 ttggctgatt aaagcagcgg caaatttatg cactgtgata agcggctttt tcagagagaa
    34981 aaactctctg gcgatgggtt tatagtttgt actgcgcaga tgttaatgtt ttgtttgctg
    35041 ttatcttatt gatatttatc ggtctatttt ttgttctcag ggcagatatc attaggttta
    35101 aagattaatc tgatctaccc atttgtgggt aaaaatacac ataatgcggg tgacataata
    35161 gttaattaac ttttgttagc gttttgaaat taaaaacacc gttcacctga agagatatta
    35221 atttttagcg atgatggagg gataattata tttgatctgg cacaagtttt actgatgaag
    35281 gatgtaactt gtgccagggg tattttgcat tacggtaata attattactt acagataacc
    35341 agcgatccgt tattggcaat attgtttcag tagtgagtag tgttctgcct gaatacggta
    35401 acggtaaact ggacgccccg tgacgccata atggatactg gtgaacaaga tgtggcagtt
    35461 gaccagccag atgaggtatt tacggcagga aacacgcgaa atgttaacct cgttggctag
    35521 ctcgtcggtt gaaaactcat agtcctgatg cgcgtcaatc cactggcaca gtgtgcgtaa
    35581 cgtctgcggc gttaagcctt ttggcaagcg acgaggatcc tgttcgttgg agctgctgcc
    35641 gtggattagc tgatcaagct cggcctggtc ataatactga tgtttttcca gcgccatttt
    35701 cttttgccgc cagccggtga gcgcctcttc aaagcgggaa gcctggaagg gtttgatcag
    35761 gtaatccacg acaccgtaat gcagcgaatc tttaatggtt gccgcatcgg ctgcggagga
    35821 gatgacaatc acatcacttt tgcaacgcgc gttatgcagg acaggcagta aatcgagccc
    35881 gttctctttt tgcatataga tatcgagcaa tatcaggtcg ataggcgtat cgctattgaa
    35941 gatcatctct ttggctttct ccagcgtcga ggctgttcca cagcattgaa agcctgggat
    36001 ttgtgctacg tatcggcgat tcagctccgc gaccattgcg tcgtcatcga taattaatac
    36061 attgatcatc tgttcgacct ctccccgtcc cagggtatct ggacaaaaaa ttgtgtgaaa
    36121 atcccgggtt ccgattccac ggcgatgctg ccgccgagat tttctacctg ttgtttgaca
    36181 agtgctaaac cgacgcctcg ctcgcttcct tttgtcgaga cacctttgtc aaaaatgtga
    36241 tcgattttgt cgggtgcgat ccccggtcca tcatcgttaa cttcgcagtg cagccagccg
    36301 tgacggtagt gcaatgttac gctaatttcg ccaccgggtt ccggccctaa tgcttccagc
    36361 gcgttttcta tcagatttcc caatgtggta atcagcgtcg cgacctggtc ctcactgccg
    36421 ctgtctggca gctggctttc actgtttaaa atcagcgtat ggcctaaatc ggtcgcgcga
    36481 ttaatcttgc tgattaaaaa accagcgata accggagatt tgatcttacc cagcagagag
    36541 ccaatctctt cctgatagtt attggctgtt ttgagaatgt aatcttccaa ctgcttataa
    36601 ctcttcagat gcaataatcc gagaatcaca tgcaatttat tcataaattc gtgggatcgt
    36661 tcacgaagtg catcagcata gttgaccaga ccgtcgagtc gctgcatcag tttacgaact
    36721 tcagttttgt ccctgaaggt tgaaatggca ccgatgataa cgccattact gcgcaccgga
    36781 acggtgttga tcagtaatag ccggtcttta atcgtaatct cttcgtcgcg gcgcggcgta
    36841 ccgtcgcgta acacttccga gacatctacc acctgtgacc atgagtggct tagcgtcgac
    36901 agtttctcat cgtcctgcga cttacggtaa ttcagcaatt cttgtgcggc atcgttgatc
    36961 agcgtgacct cgccgcgatc gtccacggca acgacgcctt ctttgataga ctgcaacatg
    37021 gcctggcgct gctcaaacag cgtggagatt tcgtagggtt ccaggccgaa aaggattttt
    37081 ttcagtacct taaccagaat gcaggtgcca atcagtccga ccagcatgcc aaataatacc
    37141 gaccagataa tgctccagcg actgtcattg atttgttggg tcacgcggct taactcaagg
    37201 ccgatcgcca ccacgccaat ttgtttatga ttttcatcgt agatgggggt aaatacgcgt
    37261 aaagcctgcg ccagaaaacc gcgattgata gcgacatttt ctttgccatt cagcgcttta
    37321 aggatgtcat cacctttaaa tggctgacca atacgctggg cttcaggatg cgagtagcga
    37381 agactttgca tatcggtaac gacaataaac agcagatcgt tgcgtttgcg cacggcttcc
    37441 gcgatggcct ggatgccgct ctcctgcggt tttttctgca agccctgacg gatttccggc
    37501 gagtcggcga gggtacgcgc cactgccagt gccttgttgg ctagcccgtc tcgcgtcata
    37561 tcactgattt gcgagaagta aatcagatgc accaccaata gcaccgagaa cagtaccgca
    37621 ctgaccatta agatcactgt ggtactcaat ttcatcggac gtttgcgtaa catgcggtag
    37681 ggcaatgaat gtctcatcag cttccttgtg tgacaaattt cttaagcatt atctctgatg
    37741 aggcgggtaa ttcaaaggga gtaagaatga ttggctatat aggggaagag attctggcaa
    37801 cggaaactgc cagtgctgta tgaagattac agggctatgc ttatagcgat aatcatactg
    37861 atgagagagg gaaagtcatg gatcaggcgc tactggacga gggttatcgc tgttataccg
    37921 gcgaaaagat cgatgtctat ttcaacactg cgatatgtca gcattctggc aattgcgtac
    37981 gtggcaacgg caagttattt aatctcaaac gaaagccgtg gatcatgccg gatgaagtcg
    38041 acgtcgccac tgtggttaaa gtgattgata cgtgcccgag cggtgcgctg aaataccgtc
    38101 ataaataagc gagggtaaaa tggaaatacg cgaaggccac aataagtttt acattaatga
    38161 cgaacaaggc aagcaaatcg ctgaaattgt ctttgtgccg accggagaga atttagcgat
    38221 tatcgaacat accgatgtcg atgaaagcct gaaagggcag gggattggta aacagctggt
    38281 tgcaaaagtc gtggaaaaaa tgcgtcggga aaaacgaaaa attatcccat tatgtccatt
    38341 tgcgaaacac gaatttgata aaacgcggga gtatgatgat attcgcagtt gatgggagag
    38401 tacagattca cgatattttt catgctctcc gcggtgtgat gcaggagagc atctgaaggg
    38461 tagggggatg cacaaagaat gggcagagag agcgtttttt tgtcccaagt catccccttt
    38521 actgagcaaa aaaaagaata tctcctatat gagaatcatc aatcggggtt aataagtttt
    38581 gcgtccccag agcgttcaat attgatagga gtcatattat ggaaggtaaa aacaagttca
    38641 atacttatgt tgtttctttt gattatccat catcttattc ctcagtgttc ttaaggttaa
    38701 gatcattgat gtatgatatg aatttctctt ctatcgtggc tgatgaatat gggataccac
    38761 gacaattgaa tgaaaattcc ttcgcaataa cgacatcatt agccgcaagt gaaatcgaag
    38821 atttaatcag gctcaaatgc ttagacttac cggatattga ttttgacctc aacattatga
    38881 cagttgatga ctatttccgt cagttttaca agtagccagg aaggataaga agaacataaa
    38941 aaatggcact attctctaaa atattaattt tttatgtgat tggtgtgaac atatcctttg
    39001 tcattatctg gtttatctca catgagaaaa cacatattcg tttacttagt gcattcctgg
    39061 tcggaataac ctggccaatg agtctgcctg tggcattact tttttctctc ttttaggagc
    39121 tgtagttgag tggacccgct ggttatcgtg aaacgaagcg tctctcctca aaactggatt
    39181 tgcggaacga cgaacgaagc agtcgcctga ctgcttcgtt cattaaagtg aaatttattt
    39241 ctgtgggcgc atcgccggga agagaataac gtcgcgaata gtatgactgt ttgtaaacag
    39301 cataatcatt cggtcgatac caatacccag accagcggtt ggcggcagac catattccag
    39361 cgcagtcacg taatcttcgt catagaacat ggcttcgtcg tcacctgcag ctttagcgtt
    39421 aacctgttcc tggaaacgtt cagcctgatc ttctgcgtcg tttaattcgc taaaaccatt
    39481 accgatttca cgaccaccga tgaagaattc aaaacggtcg gtgatttccg ggttaacatc
    39541 attacggcgc gccagcggtg acacttctgc cggatattcc gtaataaagg ttggctgaat
    39601 cagatgtgct tctgccactt catcaaagat ctccgtgaca atacgtccca gcccccagct
    39661 tttctctacc gtaattccga tagattcagc taatgcttta gcagcatcaa aattatccag
    39721 gtcggccata tcggtttctg gacgatattt tttgattgct tcgcgcatgg tgagtttttc
    39781 aaacggtttg ccgaaatcaa acacatgctc gccataagtg actttagtgg tacccagaac
    39841 ctcttgtgcc agggtgcgga acagggactc tgtcagttca atcaaatcgt ggtaatccgc
    39901 atacgccatg tagagttcca tcattgtgaa ctcaggatta tggcgaacag aaataccttc
    39961 attacggaag ttacggttga tttcgaatac ccgttcaaaa ccgcctacaa ccagacgttt
    40021 cagatacagc tctggcgcga tacgcagata catatctaaa tccagagcat tatgatgggt
    40081 aataaacggg cgagcagatg ccccacctgg aattacctgc atcatcgggg tttctacttc
    40141 cataaagccg cgcgcgacca tgaactgacg gatagcggcc agaatttttg aacggacaac
    40201 gaacgtttga cgggatttat cgttagcgat gaggtccaga taacgttgac gataacggac
    40261 ttcctgatcc tgcagaccat ggaatttatc tggtaaagga cgtagtgctt tagtcagcag
    40321 gcgcagctca gtacagtgaa tggaaagctc acccgtttgc gtcttaaaca gcgtaccgcg
    40381 ggcaccgata atgtcaccca gatcccattt tttaaactga tcgttataaa caccttctgg
    40441 caggctatct cgtgcgacgt acagttgaat acggccaccg acatcctgca acgttacaaa
    40501 ggaggctttc cccatgatac gacgggtcat cattcggcca gcaaccgaga cttcaatgtt
    40561 taaggattcc agttcctggt tatccttcgc atcaaactct tcgtgcaact ggtcagaggt
    40621 atggtcgcgg cgaaaatcat tgggaaacgc cacaccttgc tgacgtagtg ccgccagttt
    40681 ttcgcggcga tttctcagtt catcgttaaa atcaatagcc tcattggctc cccgtgtttc
    40741 ttgttcagac attttggttc ctctaaatcc agctttcaat ttagcgtaga taaagagaca
    40801 gatcggtaca ctgtattatc tgcctcgact attaataact cagtttatct tatcgaaact
    40861 aacgcttacc tcaagttgat atcactaata aataaccatc aaaatggttt aaatgactta
    40921 ttaatctcga tttaatagtg tgataaactg aaagttaatt tattcataac ctattgtttt
    40981 tacgacagtt aatcgttgct ctcctgtatc atattcgtcg gcgtgctaaa cagaaaacgg
    41041 gtagcaaagg ccaataccac gatgatcgcg acacaagcca acgtccactc tcccatctga
    41101 gaaaagaatc gctggtaagc tgcaattgcc ataccgctaa tttgcgactc tgtcgtctgc
    41161 tgtgccacga cgcctgccag ccagttggcg accgcgcctg ttgccagcat ataaatcccg
    41221 gttaatacgc cagacatttt cagacgcgtg atttgtgcaa tcgccaccgg gtcaataaag
    41281 agttcggcaa agcccattag cgccaacccg gatatcatca cgcccattga cgcttgaccg
    41341 tcagccgctg catgtcgggc atcaaatgcc aacaacataa agccacaagc catcagcagt
    41401 aagccaaagg caaacttcag ccagacgcgc aatgttgagt tgccgcggct ttctggcgac
    41461 gccagccagg ccagtacaac cccagcgagc atcaccgcaa ttgcattcac cgactggaat
    41521 agtgctgtag gtacttcaat attgaatgcc tgacgattca caaagcgatc gataaacaag
    41581 ctgatggtac tgccgccctg ttgtgccagt acccagaaca acgtcccgac aaacatcaac
    41641 aatacaattt gccaaagagc acggcggtgt tcggggaatt tgatcatcat gcgggcaatg
    41701 atttgtgcgg caatgaggca aacgatcgcc agcaaatatc cggaccagtc gttctccagc
    41761 agcagggtaa aaaatactgg ggctaaacag agcatcacca ccaaccagct ccataccggt
    41821 aaggcaaatt tgacgctagt gagcgctttt ttatccatac tacgcgtgga ctggaaatga
    41881 cgatgaccgc ttaagaaaat caacaaaccg ataaacatgc cgccacccgc aagggcaaag
    41941 ccaacatgcc atccatacca ctgagcagcc aggccgcagg cgattggggc tgcgatagaa
    42001 ccgatattgc ccgcagcata cagcagcgaa aaaccgccat cacgtcgatg atcgttctcg
    42061 tcgtaaagct cgccaagcaa acagctgatg tttgatttga ataaaccgta gccacaaata
    42121 atgattgcca gcgccagata caggctaaag gttgaatttg tatcaatgcc cagcaccaca
    42181 tgaccaaggg tcattaacag cgcgccggca atcactgcag tgcggttgcc gagcaggcgg
    42241 tcggcaagcc agccgccgag aataggggta acgtaaacca gagaagcata tgcgctgaac
    42301 aggctgatgg catggttatc atcaaaacca agctgatggg tgagatagag aatgagtaag
    42361 gcacgcatgc cgtaaaaact gaagtactcc cagatttgga tcgccacgat atagtatatc
    42421 gcgcgcggct gtgagggtgt tttcatgtgt tctccttatg agcaaaaaag ggaagtggca
    42481 agccacttcc cttgtacgag ctaattattt tttgctttct tctttcaata ccttaacggt
    42541 atagcggcca tcagcctgac ggtatgcacc gtgaatatca gtttcaaagc ccggatagtg
    42601 agcgccgatt tcacacagca tctgcaggaa ctccagaacc ggacggcttt cttcggtgat
    42661 catttcaccc ggcattacca gaggaactcc cggcggatac ggaaggatca tattggcgtt
    42721 aatacgaccg accatttcgt cgaggtaaac ttcttcggtc ataccgtgca gctctttctg
    42781 gaacgcagca tacggagtca ttaccatcgt cggcagcact tcaaatgcgc gatacatcag
    42841 atccggcaga ttgtggtgaa caatcagttt gtggatattc tgagccagtt cctgaatacg
    42901 catgttttca tagaattcag gatcttcacg atacagagac ggcagcatgt ttttcacacg
    42961 caggttcagg tcgaacgcac gtttgaagtc agtcagagca cgcagcaagc tcagtgcttt
    43021 ggtcttatcg ataccgatgc tgaacaggaa cagcaggtta tacggaccgg ttttctcaac
    43081 aacgatgcca tgttcgtcga ggtatttcgc cacgatgctg gccggaatac caaagtcgct
    43141 catggtgccg tctttttcca tccccggagt cagcagggtg actttgatcg ggtcaagata
    43201 catgtgctcg ttatcgatgt ttttgaagcc gtgccaggtg ctgtcagaac gcagcggcca
    43261 gcattcagtc gtatcgatat gatccggctg ccaaacatca aagaaccagc catcagattc
    43321 cgttctcaga cgtttgatct ctttacggaa tttgatcgca cgttcaatgg aaccgttgat
    43381 cagacgctta ccagcattac ctttcatcat cgccgcagcg gtttcagtgg acgccacgat
    43441 accgtagtgc ggagaagtgg tggtgtgcat catgtaggct tcgttaaagg tttcttcgtt
    43501 tacgtcacct ttaacgtgga tcatggaagc ctgagagaac gccgccagca gtttgtgagt
    43561 ggactgggtt tcgtaaatca ctttcccttc tacacggcca ccgctcatac cgcatttacc
    43621 ttcgtaaatc ggtgagaagt tggtgtaagg cacccacgcg gagtcaaagt ggatggattt
    43681 cacatccagt gttttcttga tgaagtcggt gttgtacagc agaccatcat aggtagagtt
    43741 ggtaattaca gcatgtaccg gccaggttgc gtttggtgtt tctttcacgc gcttagcaat
    43801 ggtagcgtgc tggaattcac tctgtgggat accaccaaga ataccgtaag cgttacgggt
    43861 cgggcggaaa tagattggcg taacatcgct catcatcatc aggtgggtca gcgatttgtg
    43921 gcagttacgg tcaatcagaa tggtgctgcc tgccggagca gagtacatgc caacaatttt
    43981 gttcgcagtg gaagtaccgt tggtcaccat gtagctgcgg tctgcgttaa agacgcgagc
    44041 gatatactgt tctgcttctt tgtgtggacc actgtgatcc agcagagaac ccagttcaga
    44101 tactgaaatg gaaatatcag atttcatggt attcggacca aagaaatcat agaacaggct
    44161 acctaccggg cttttctgga atgcagtacc gcccatgtga ccaggagtac agaaagtata
    44221 tttaccttca cgaacatatt taaacagtgc tttagtcagt ggaggcagaa tagtgttgat
    44281 atattcgtca gtggtctgct tgatcttatt agcaatatct tcagcagcac ccagcgcata
    44341 ttcaaagaag ctaatctgta aacgcaggtc attcaggctt acatcgagag tggaatacgt
    44401 attagcgaac gcgtacaacg gcaggttctc gttcattttg ctaatttctt cgcacagctc
    44461 gagattatat ttatcccagt caaaaataac gccgcacaga cgcgcattgt tttcgatcag
    44521 ttttaataag tcgtcacggt cgttcgggta aacaatctgg aagttcagac gttcaagcgc
    44581 gcgatgaagt tcacggatgg gttcttcttt aaaataaacc cccatgtgat tcaatattgc
    44641 aataacgttc atagtcatat ctccaggtaa aaaaggcccc tcccaacaca tgggacaaaa
    44701 tgaaaggagg agcctcggaa aatactttta attaatgtgc gttagacgcg gtgtggttat
    44761 ccattgagtg gctctggcgc tcgtgcattt tgcgagcgta gaacatcagg ataatcaggc
    44821 tgacgatgaa ggtacctgcc agctcgaagg agcttgcgcc catcagcgcg atgaagcaga
    44881 acacacaacc cagtacagag cagatcaggc tgacaaagtt gcggatgtta acgccttcaa
    44941 aacgaatcag gtcaacgcaa gagtagaagt acggcagcat agtcagcagt actgcgatac
    45001 cggtcagttc accgaacagg tcagatgctt taccaccggc agagttcatc agagtgatga
    45061 ggatcatcag ggcagtcatt ttcactgcag ccagcagcag accttttttc ggaataccgt
    45121 tgctgtcgac ttcaccataa actttcggga agttaccgtc gttagcggca cgtacacctg
    45181 cctggcctac caacatcatc caggagccca gagaagtcag gcacgcaaag gcggtgaatg
    45241 cagaaaccag cggcgcagcc cagttaccga ggatggttga agcactgatt gcaaacggag
    45301 caccggaagc tgccattaca gaagatggat acataccgga aagcacctga gtcgcagcga
    45361 tgtaaacaat acctgctaaa ccagtaccca gcatggttgc cagcggaacg gtacgtttcg
    45421 ggtttttaac cataccagta cttactgctg cggattcaac acccacgaag gcccacaggc
    45481 agagcagaat acttttaatg atcgcatgac catcagtggt atccgcagta ttccagttag
    45541 ctgcataagt tgccgcatca aaccaatgcc agccaacaat agcggtcatt actaccggaa
    45601 taagaaccag caccagacca atagtggtta aacggcttac ccaagtaccg ccgagcatat
    45661 ttacaaaggt aaatacccag acgatagcaa tacaggcgat acccgccgga acaggatcat
    45721 ttaatactgg gaagaaggtg gaaagataag ataccgcggt aataccaatc gccaggttac
    45781 caatccagtt agcatggtaa taaagaacac ctgtctgaaa accaaatgca ggggaaattt
    45841 ctccggcata agcaattggg ccaccttgtt gcgggttttt tgttgccagt cgggcatata
    45901 catacgccag cgacattgca ccaataatag agataatcca accccagata gcaataccac
    45961 cgatacttgc taggttcgca ggtaataatg caataccgct ccccatcata ttaccggcaa
    46021 caacaccggt acaggcaaat agcccgatct tcttggcaga actcatgctc ttctcctaat
    46081 ttcatttttg aatttggagt ccgggtcatg atgtataact atttcctgac cagaccaaac
    46141 tggcgctaag aatactcacg aaaaaaggat taatcctaaa gattaggtga aataacacaa
    46201 aagtttctgt aagtgagaac ttgaggtttt ttattaacac atcaggatcg caagttgata
    46261 tcatgaaaag ataaacattc aatgtttaca atggattgcg ggatattctc tggttaaatt
    46321 tatgtaataa aaattatgcg gcaaataaat tgccgcaaca tattatacca acagaaacat
    46381 acaaaaactc aacaacaaat attcccgagc ataaatcaac ccgggagtta cttattctga
    46441 agcgagaaat ttgtcgagat aaggtacaac ataaggaaca gaagtctgga atataccatt
    46501 ttcaatccag taaagggtgt ttgcccctgg gcgtaaatta aaggcggtga gatatgcatc
    46561 agccgcttcc cggttcatcc ccttcatttc ataaaccttg ccaagcaaca cataatttag
    46621 ccaggacatt tcaagatcaa tgccagtatt tatcgcctgg taagactcat ctgttttacc
    46681 ttttaccaga gcactaaccg cttttatttg atatataatg gacaggttat tcaattccgg
    46741 cagtgtaaca atgttgtcta tttctgtgtt cagtgctgct aattgttttt catctaaagg
    46801 atgttgagaa tggcgcacga tatcaactaa tgctttttct gctctcgcgt aggtaaattc
    46861 tggggatgat tgaacaatct cacctaataa ttcactggca cgattcaatg atttgtcatc
    46921 gccatgcagt aaataatcat gagcctgata aaaattagtt aataacgcac cacgatgagg
    46981 caaaatttgc tggagcgtct cctgcattcg ttgtggccac ggttggttta acgcttttga
    47041 taaactctcc agtaaatcat tttgaatcgc cagctgatta ccgttagtga tgacataacg
    47101 tttatccagc atggttgaac catctgcatt gtctaccaat tttatcgaca taaagcattg
    47161 ttgagcacgg tattggcgct gattaacaaa ggcaatagat aatgttttac cggaactgct
    47221 cggttcatca atgttgtagt tgattttgtc atgcaccata aaggtggaga aggtgttaag
    47281 tgatgtcgcc accaaatcac ccacgcctat cgcgtaagag agctgatacg gggaactcca
    47341 gctgttacaa cttttattta ccatattaat gtcaatatcg cgtggattga gcaaaatacg
    47401 cgatttgctc ataggaagac gtgtatcaag agttgaaaac gctaccagtg ctacacagat
    47461 acctaacgac aacaggaaaa aaaaccatac ccaaaaggta gtgaatcgtt tgcttttaac
    47521 tggggattgt tcaggtggcg ttgcggtgtt ttgaatgtta agactgtggg agggagaatc
    47581 tgtggcagga accgcctctg gtataggggg aggcgaagat agcattattt cctctccctc
    47641 ttcttcgctg taccagataa ccggcaccat taatttatag ccgcgctttg gtacagtagc
    47701 gatatagaca ggactatctt catcattatc ttttaatgac ttacgtagtt ctgagatact
    47761 ctgcgtcaca acatgattgg tgacaatact tctcttccag acattatcga taagttcatc
    47821 cctgctaagt acttcgccac tgtgttgagc aaagaaaacc agaagatcaa ttaatctcgg
    47881 ctcaagggta agttgacgcc cattgcggct aatttggttt atggacggag taacaagcca
    47941 ttcgccaacg cgaactacag gttgttgcat aatagaaact cattcgaaaa gggaatggtg
    48001 caatgataat tgccatgacc tatttttacc atcaatagat gggtttattt acatgttatt
    48061 ggtgaatgca agacgttatt tttaccagcc ataaacttct tgattacata gtattacgaa
    48121 aggattttac tgagaaccag aagtaatatt ccttaccatc aaaattcatc atctttgcca
    48181 aagaaaaatg ttcagaaaat aatccatgga aaattgtccg gagcgcttac tattttaatg
    48241 gattgttagt ctttgcatga gcaagcggac tgatacattt ctctttgttc tcattcagaa
    48301 aatctcatca gtcggcgttc agaccggtgt tgtgctttat ggatgcgtta cgctcctgat
    48361 gacgtcattt gacgttcaac agcatcacgg ggccgcacga catttcacgt cagttagtgc
    48421 tatagctcag gaacaaattt tcccaaattg ggatatgctc gcaaattgct ggtgatgtgg
    48481 ggagaatctg gttgagttcg gtagaattga tttggaggca gaacgcttaa atcgtggcgt
    48541 cctgaaacga aaaacggacc tccgtggagg tccgttgata tgaatttggt gcccggactc
    48601 ggaatcgaac caaggacacg gggattttca atcccctgct ctaccgactg agctatccgg
    48661 gcaacggggc gcattaaacc gtaatctgca catctcgtca acctaatttc agtaaaagcg
    48721 attcaactgc ttaagattgc ggcaaatcgc ttcttttctg tgtttctgtc aggtcaatgc
    48781 accaccctgg cggcagcggg cgaagcgcag gatatcttcc gccagccgat gtgcagtgtc
    48841 gacatccgcc tggctacgat tcaccagcat tcggcttaaa cagccttcca gcaccagttc
    48901 catctgcttt gctaccatcg ccggatcgtc aacttccagt gtggttaaca gttcgtgggt
    48961 gaaatcgtag gccgcgcttt tttgctgatc ggccagttga tgaatagggt ggccaggatc
    49021 gggataaaac gtacaggcag cgataaatag acagcccgga tagcggttgt ttttaacgca
    49081 ctccgataac gcctgataac gtgccagcag cttttgttcg gcggtttgcg tttcgtccag
    49141 catcagctga cgacgcgaga catctatctg ttggctaaga taacgcagcg catcgtagag
    49201 gattgcctct ttgtctggcc agaagcggcg tagctcgtcc agtggataat ccacacgttc
    49261 agcaaccatc tccagcgtgg tgttggcaat cccttgtaat tctaataatt tcagggcttc
    49321 tcccagtaca tcttcacgtt gcacgctatt ttcctccgtc tttcccactg caagtgtcgt
    49381 tcacggttgg cgatcgcgca aatgtgcgct gaaggtttca gcatccataa agcccgtgac
    49441 gcgtgcttgt ggatgctcct ggccttgtcc gtcaaaaaag agaattgtcg gtaggccaag
    49501 gacattaaga tgctttaaca gcgccacatc ttgtgcgtcg ttggccgtga cgttggcctg
    49561 aagtaagacc gtgtctgcta acgctttttg cacctgcggg tcgctgaagg tgtatttctc
    49621 aaactcttta caggcgacgc accagtcggc ataaagatct aacatcaccg gtttgccttt
    49681 ggcttcaacg agcgcctgat ttaactcatc taccgttttg atttgtgtaa agttgagatg
    49741 cgtctgagtt tgcgcggtat gcgtcgcacc aaatgcccaa tcctgaagtg ggcgcacgct
    49801 aaccaatgcc gctgccagca ggattatttg caccacgcgc atccagccgc gtttagcctg
    49861 taggctggtg ataaaggccc agccaaagaa tgcgacaccc agcgccgacc acaagcgtaa
    49921 tccccatata tcaccaatca ctcgctccag caggaagacc ggcagtacga ggatcacaaa
    49981 accaaacgcg gttttgactt gttccatcca cgggccgctt ttcggcagca agcggttacc
    50041 aaagacggta atgagcatca gcggcaggcc catgcccaac gcatagagat aaagcgtgcc
    50101 gccgcccagc cacatgttcc cgctttgggc gatatacagc agaatcgcgc taagcggtgc
    50161 ggtggtgcat ggtgaacaga tcagtccggc aatcgccccc ataacaaaca caccgccagg
    50221 tgagccgccc tgttggcgat tgctcatcag cgtgaggcgc gtttgcagcg aggaggggag
    50281 ttgtagagta aacaagccaa acattgacat cgccagcaag gtaaagacga tagcgaggcc
    50341 aatgagcacg tatgggtgct gtagcgccgc ctggaactgt aaccctgcgg cggcaaccac
    50401 cagacccagt gccgtgtaag tcagcgccat cccctgcaca taaataaagg tcagcaacaa
    50461 tgctctggca gtggaaagcc gctgtttacc gcccagcacg atgccagaaa tcagtgggta
    50521 cattggcagc acgcatggcg taaaggcgat accaataccg atcaacaacg cccagagcgc
    50581 ggaaaagggc aattgcgcgg tgggctgctc ttgctgcgga acagacacag gctgtgacgc
    50641 tgcgttgttg gtgaccactt cgcttaacgg aacggttttg gtttctggcg gataacagaa
    50701 accggcatca gcacagccct ggtaggtgac agttaacgtc gctcccgcac tcgcctggtt
    50761 gatggttacg ggaagcgtca gccgatcgcg gtaaatctcg cttttgccgt aaaactcatc
    50821 ttcatgccag acgccttgcg gcagctgcac gtcggcaatt ttcgcgtgtt ccgacgtaat
    50881 gcggatctgt ttacggtaga ggtagtaacc gtctttgatc tgccaggtca gattcaggtc
    50941 atgttggttt tgctgaaaat caaaagcaaa tgcttgatcc gcggggacaa attgtgaacg
    51001 tcccggcgcg tcgaataatc cggcaaaaac ggaagtgctg caaagtagca ggatcagcgt
    51061 aaagatgcgt tgagccatga gaggtaatct gtgtctccgt gtgtaacagg taaaaccaga
    51121 agttccgggg tttgatatgg atgatgagac ttcaggcatt ccagcagtgc ctgctggtga
    51181 gatacggtag tttttaaaat catctgcact tcgtattctt gctccagctt accttcccag
    51241 taatagagag aggtagcgcc ggggatcaag gtcgcgcagg ccgccagttt ttccgccagc
    51301 actttggcgg ctaaatcctg ggctgtcgct tcatccggtg cggtacatag caccacgaca
    51361 gacgcggtat tcgaactttt ttcatcaagc ataaacacct cgcaagaaca gatggaaccg
    51421 caagagagag gtcactatac aacggacggg ggaaggatgt tagtcggcgg gaaagaaagc
    51481 ggggcgtgaa cgccccgcga ttggtcatta cagcatgaag ctacccagca cgaagccgaa
    51541 gcaaacggcc agggcaacac ccagagtacc cgggatgaag aacggatggt tgaagacgaa
    51601 tttaccgata cgggtagtac ccgtgtcatc catctgtacc gcagcaacca gcgtcgggta
    51661 ggtcggcaga atgaacagac cagacaccgc agcgaaagaa gcaacagcgg tcagcggaga
    51721 aacgttcagt gccagagcca tcggcatcag tgcttttgcg gttgcagcct gagagtacag
    51781 cagagcagaa gcaaagaaga agatgacggc cagcagccac ggatgaccct gaatcacttc
    51841 accagcggta tctttgatcc agtcgatgtt gttggaaacg aaagtatcgc ccagccacgc
    51901 aacacccagg atacaaatac aggcgctcat acctgctttg aaggtgctgg agttgaggat
    51961 gttgtcggta tcgactttac agataacggt ggtcagagtt gcaacgctga gcatgatgat
    52021 caggattgcg ttggtggtgt tcatcagcgg tttttcaacc agacccatgc ttgggctgtt
    52081 gatgattgca tagataacca cgccaactac gcccagcagg aacagccaga cggacgtttt
    52141 tgcaccggat ttgatttcaa tctgcttttc accgcgcagt tcaaccaggc cctcttccag
    52201 acgcttgcga taaatcggat cgtcagagag tttggagttg aacagcatag tgaccaggaa
    52261 ggacatcacc agaaccgcca gcagggtgga cgggatgacc acggagagca gatggaggta
    52321 gctgatgcca tgaccttcca tcacggaaga catgtaaacc actgccgctg agattggcga
    52381 tgcggtgatc gcaatctgcg cggataccac tgcagtagac agcggacggc aaggtttaac
    52441 gccttgttcc ttcgcaactt cagcgataac tggcagtgtc gccagagaga tgttgccagt
    52501 accagcaaag atagtcagga aataggtcac gatcggtgcg aggatcgtga tgtatttcgg
    52561 gttacggcgc agcagctttt ctgtctgatg aaccagatag tccagaccgc cagcaacctg
    52621 catggcagaa atagcggcga taaccgccat gataatggaa atgacatcga acgggatgtt
    52681 accgggttta acgccaatag cggcaagaac cagcaccccc aatccgcctg caaaaccaat
    52741 acctattccc cccaatctgg cgcccaagaa gatcgccagc aaaactatga tgagttctac
    52801 aactagcata ttagccttcc ttgtttttta acaagttgat attagattgt tatttttaag
    52861 ttactactca caagaaaaaa gacacgtcat ctgacgtgcc ttctctattt gtactaccct
    52921 gtacgattac tgttcgcttt catcagtata gcgttttgct ttgtaagccg ggtgcatcag
    52981 attctgtacg gagaaaatat cgtcaagttc cgcttcagtc aacaaaccgc gttccagaac
    53041 gacttcacgt acactcttac cggtttcggc acagatttta cccacaatgt caccgttgtg
    53101 gtgaccgatg aacgggttca ggtaagtaac aataccgata gagttgtaaa cgtaaccttc
    53161 gcacacttct ttgttagcag tgatgccgtt aatgcatttt tccagcaggt tgtagcaagc
    53221 gttggtcaga atgtgaacgg attcgaacat agcctggcca atgaccggct ccataacgtt
    53281 caactgcagc tgacctgctt ctgctgccat ggtaacagtg gtgtcgttac cgatgacttt
    53341 gaagcatacc tggttaacca cttccggaac aaccgggttt actttagctg gcatgatgga
    53401 agagcccgcc tgcagttccg gcaggttgat ctcgttcagg ccggcacgtg ggccagaaga
    53461 gagcaagcgc aggtcgttac agattttgga catcttcaca gccaggcgtt tcagcgcgcc
    53521 gtgaaccata acataagcgc cgcagtcaga ggtcgcttcg atcaggtctt cagccggtac
    53581 gcatgggaag ccagtgactt cagccagttt tttcactgcc agcggagagt actctttcgg
    53641 cgtgttcaga ccagtaccga ttgctgttgc gccaaggtta acttccagca gcagttcagc
    53701 ggtacgttgg atatttttca cttcttcttt cagcaggatg ctgaaagcgc ggaattcctg
    53761 accgagggtc atcggtactg cgtcctgcag ctgggtacga cccattttca ggatgtcctg
    53821 gaattcgaca gctttacgtt caaagccttc acgcagttgg ttaatcgcat ctaccagctt
    53881 aatcagggaa gagtaaactg cgatacggaa accggtcggg taggcgtcgt tagtggactg
    53941 acatttgtta acatggtcgt tcgggttcag gtactgatat tcacctttct ggtgacccat
    54001 cagttccaga ccgatattgg ccagcacttc gttggtgttc atgtttacgg aagtacctgc
    54061 gccgccctgg tagacgtcta ccgggaactg atccatgcat tttccgttgt tcaggacttc
    54121 atcacatgcg gcaatgatgg cattcgctac acttttagga atggtttgca gctctttgtt
    54181 tgccatagct gcggcttttt taaccattac cataccgcga acaaattcag gaatatcact
    54241 gattttgttg ttgctgatat agaagttttc aatcgctctc agagtgtgaa caccatagta
    54301 ggcatcagct ggaacttccc tggtacccaa cagatcttct tcgatacgaa tgttgtttga
    54361 catgtgaacc ttctttttca agctgccaat gatttgcttt aaacacacag aatatatgtg
    54421 gtttcgaatg tttttcgacc gacgattatc ccctgcatcg accgaatacc cgagatcata
    54481 tgctgcttga ggatttctac cgtaatctgg atcactttaa gtgtcggttt ttacccctta
    54541 attattaatt tgtgaaatag atcaccgctt tgggattact accaaaaata gttgcgcaaa
    54601 catcttgaaa ttttgctaat gaccacaata taagctgaac gcgattcgca acccattcag
    54661 gtagccgggt tttaccggct gctattacag gagaaacctt tgcgctggtt accttttatt
    54721 gccattttcc tttatgtcta tattgagatt tcaatcttta ttcaggttgc ccatgtattg
    54781 ggggtattgc tgaccctcgt gctggttata ttcacgtcag ttatcgggat gtcactggta
    54841 cgtaaccagg gctttaagaa tttcgtgctg atgcagcaaa aaatggcggc gggtgaaaac
    54901 ccagcggcgg agatgattaa aagtgtttcg ctgatcattg ccggtttgct gcttttatta
    54961 ccgggctttt tcaccgactt cctcggtctt ctgcttttat taccgccggt gcaaaagcat
    55021 ctgacagtga agttgatgcc gcatttgcgc ttttctcgta tgccaggcgg cggttttagc
    55081 gccgggaccg gtggcggtaa tacttttgat ggtgagtacc agcgaaagga tgatgagcgc
    55141 gaccgccttg atcataaaga cgatcgccag gattaatgtc gaaacgccgg attatgtggt
    55201 tatgccattt tccggcgttt ttcgttttgg cagaaacagc cataaccccg ccagcatgat
    55261 cagcgcatag agacttttcc agccgaccat tgccagtaac agaacgcata acagcccgcc
    55321 aaccaccgcc agtagtcgat aacgtccttg caataattta cagcctgcca gcatgcataa
    55381 cagataaatc ataataaaga tgccattggc ataaataata agagcgtcca gattgatctc
    55441 taaagcatga atcaccaacg tgctcaccac gcagcagccg agcaccgcat tgagggcatt
    55501 attcgggata tggcgagaag agaggcgtgc caggtagtgg tcaggattat gttgcgcctg
    55561 cgaccagacc aggcgggcga agctctgtat ataaatgttg agactggcaa agcaggccag
    55621 atagccaatt acgcaggcaa tccataacgc tcctacaccg aacagttgca ctacaatttt
    55681 tggaagcgat gctgccgccg ccatttgttc accataggcg tcgaagtgta agacgactac
    55741 cgtacagccc cagtagacta atcctgccag cagcagacca atcatcaatg cgcgaggaaa
    55801 atcacgctct ggatttttaa attccgaggc aagatgggca aatgcttcca gaccgacaaa
    55861 acaccagaac atcactgata acgcagcaaa taacccggta agttcgatat ttcctggcgc
    55921 ggggaagggg atactcgcag gtttgatatc gcccgcccac cagatagcga caatcagtgc
    55981 gacgataagt ccggcaataa cggtttgtag attagcactg gaactggcac cgcgagtacc
    56041 gatataccac accagcgcca gcgtaccgag ttctgccaac aacagttgcc agctatgcca
    56101 gccaaacatt gcctggccga acccggcggc aatttgtagc gcggcaggca aacccacggg
    56161 aatgaccgat aaaaacaacc agccggtgac tcgctcaagt cgcgaaccaa acgccatacc
    56221 gacgaagtgc gcgacaccgc ctgcgctggg atagtggcga cccagaatcg caaacacaat
    56281 cgcaatcggg aacactaaga taatcaaaac gggccacgcc cacaggctgt tattgcccgc
    56341 taccagcgca gctaacgcag gaacggcaaa cacgccagtg cctaataatg acgtcgatag
    56401 caggccaatg ccctgggcca gccccagttc ttgtttgagt ccactcatgg gttgatatcc
    56461 gattgcgccc aaattttggg caactgcgta gattttcgat ggtagcacaa tcagattcgc
    56521 ttatgacggc gatgaagaaa ttgcgatgaa atgtgaggtg aatcagggtt ttcacccgat
    56581 tttgtgctga tcagaatttt ttttcttttt cccccttgaa ggggcgaatc ctcatcccca
    56641 tttctctggt caccagccgg gaaaccacgt aagctccggc gtcacccata acagatacgg
    56701 actttctcaa aggagagtta tcaatgaata ttcgtccatt gcatgatcgc gtgatcgtca
    56761 agcgtaaaga agttgaaact aaatctgctg gcggcatcgt tctgaccggc tctgcagcgg
    56821 ctaaatccac ccgcggcgaa gtgctggctg tcggcaatgg ccgtatcctt gaaaatggcg
    56881 aagtgaagcc gctggatgtg aaagttggcg acatcgttat tttcaacgat ggctacggtg
    56941 tgaaatctga gaagatcgac aatgaagaag tgttgatcat gtccgaaagc gacattctgg
    57001 caattgttga agcgtaatcc tcgcacgaca ctgaacatac gaatttaagg aataaagata
    57061 atggcagcta aagacgtaaa attcggtaac gacgctcgtg tgaaaatgct gcgcggcgta
    57121 aacgtactgg cagatgcagt gaaagttacc ctcggtccga aaggccgtaa cgtagttctg
    57181 gataaatctt tcggtgcacc gaccatcacc aaagatggtg tttccgttgc tcgtgaaatc
    57241 gaactggaag acaagttcga aaatatgggt gcgcagatgg tgaaagaagt tgcctctaaa
    57301 gcgaacgacg ctgcaggcga cggtaccacc actgcaactg tactggctca ggctatcatc
    57361 actgagggtc tgaaagctgt tgctgcgggc atgaacccga tggacctgaa acgtggtatc
    57421 gacaaagccg ttaccgctgc agttgaagaa ctgaaagcgc tgtccgtacc gtgctctgac
    57481 tctaaagcga ttgctcaggt tggtaccatc tccgctaact ccgacgaaac cgtaggtaaa
    57541 ctgatcgctg aagcgatgga caaagtcggt aaagaaggcg ttatcaccgt tgaagacggt
    57601 accggtctgc aggacgaact ggacgtggtt gaaggtatgc agttcgaccg tggctacctg
    57661 tctccttact tcatcaacaa gccggaaact ggcgcagtag aactggaaag cccgttcatc
    57721 ctgctggctg acaagaaaat ctccaacatc cgcgaaatgc tgccggttct ggaagccgtt
    57781 gccaaagcag gtaaaccgct gctgatcatc gctgaagatg ttgaaggcga agcgctggca
    57841 actctggttg ttaacaccat gcgtggcatc gtgaaagttg ctgcggttaa agctccgggc
    57901 ttcggcgatc gtcgtaaagc tatgctgcag gatatcgcaa ccctgactgg cggtaccgta
    57961 atctctgaag agatcggtat ggagctggaa aaagcaaccc tggaagacct gggtcaggct
    58021 aaacgtgttg tgatcaacaa agacaccacc actatcatcg atggcgtggg tgaagaagct
    58081 gcaatccagg gccgtgttgc tcagatccgt cagcagattg aagaagcaac ttctgactac
    58141 gaccgtgaaa aactgcagga acgcgtagcg aaactggcag gcggcgttgc agttatcaaa
    58201 gtaggtgctg ctaccgaagt tgaaatgaaa gagaaaaaag cacgcgttga agacgccctg
    58261 cacgcgaccc gtgctgcggt agaagaaggc gtggttgctg gtggtggtgt tgcgctgatc
    58321 cgcgtagcgt ctaaactggc tgacctgcgt ggtcagaacg aagaccagaa cgtgggtatc
    58381 aaagttgcac tgcgtgcaat ggaagctccg ctgcgtcaga tcgtcctgaa ctgcggcgaa
    58441 gaaccgtctg ttgttgctaa caccgttaaa ggcggcgacg gcaactacgg ttacaacgca
    58501 gcaaccgaag aatacggcaa catgatcgac atgggtatcc tggacccaac caaagtaacc
    58561 cgttctgctc tgcagtacgc ggcttctgtg gctggcctga tgatcaccac cgagtgcatg
    58621 gttaccgacc tgccgaaaaa tgatgcagct gacttaggcg ctgctggcgg tatgggcggc
    58681 atgggtggca tgggcggcat gatgtaattg ccctgcacct cgcagaaata aacaaacccc
    58741 cgggcagaaa tgtctggggg tttttctttt ggtcatcttt ctagtataag attcagacac
    58801 ggacgacgcg agtggcgtcc agctcattga ttatggggaa taacatgcac gtaaaatact
    58861 tagcagggat tgtcggtgcc gcgctactga tggcgggttg tagctccagc aacgaattga
    58921 gtgctgccgg tcagagtgta cgcattgtgg acgagcagcc aggcgcagag tgccagctga
    58981 ttggtactgc gacaggtaag caaagcaact ggctttccgg gcaacacgga gaagagggcg
    59041 gttctatgcg cggcgcagca aacgatctgc gcaaccaggc ggctgcaatg ggcggtaacg
    59101 tgatttatgg catcagcagc ccgtcgcagg gaatgttgtc cagttttgtc ccgacggata
    59161 gccagattat cggtcaggtt tataagtgcc cgaactgatg tgatccagag gctgggtgac
    59221 aaacgtatca ttgcctgatg cgcttcgctt atcaggccta cagcaggcat tgcaactgat
    59281 tgaatgcttg agaatttgta ggccggataa ggcgtttacg ccgcatccgg catgaccaac
    59341 gatcacgtta tcagtcattt caggctggat ggctccagcc tcgcattagc aaagatgaga
    59401 ttatttcgcc tgtggtgcag ttttgctggt ggattgaaca cgtaagtgat gcagcgcgcg
    59461 taagcattga tcctgggtta gcggttgggc gttattcgcc gctacggtgc gaacatagac
    59521 ctgacatggc acacccgcga gttttttcag tcgttggctg aaatcaagca ttcggcgagt
    59581 gatcgcctct atttctttac ccggcgttgc ttcacgcgtt ttcaccactg cgccgctaaa
    59641 ttgttctttg ccactttcat ctttaaagcg gtaaagcacc gccagataat ggtcgaacag
    59701 gttatgtttc cattccggtt gggaataggt gtcatattcc agattgccat tttcctggca
    59761 gtcaacgtca tataacggca ggaaatctaa cagtgaggta atacgtaatg ccagttcacg
    59821 catttccgca ttattaattg catgaatgat tgccgcgcca gcatttcaat ctgcaactca
    59881 ttgacgacta actcacattt gctaccgtca tgtagcgtta atacgaaggt aagattttcg
    59941 ccttgattat cggtaagttc cagcgtgtta acccgacgat taatatcggc atttttaagc
    60001 tcatcaacca gaatttctgg gatgttttct gccattttct taatgacttt atcgcgagct
    60061 tgctcatact gaaggcgtgc agcagcatcc agtttatgct tttgatgcat acgactttcc
    60121 agcgcaatca gcaggtcgcg cagttccatg acagacataa agaacaacga ttctttattg
    60181 cgaggttctt taattttcag cgccagtgca atgaaattat tactcttgcg aataacaccg
    60241 gtattaacac ccttgatgct tatggccatg taggttctcc ctaaccattt ctcaattaaa
    60301 taattaattt taatttataa gccagataaa tgggcttggt agtaatagtt gttaaaataa
    60361 cataaatagc cgtactcact ctatataaac agaaaagaaa ggttaattat tggtgttggc
    60421 tatataaaga gtaacaacag caatgtatat gaataattaa aggttatgca acgggcaaag
    60481 attaaatttc ccagtttaat gacaggttgc tgtacttgcg tttgttgctc ttgccggatg
    60541 cggcgtaaac gccttatccg gcctacagtt cattgcaaat ccattcaatt tcaatgcaca
    60601 atgtaggcct gataagcgta gcgcatcagg ctaatgttta acttccctgt ttaatcagtt
    60661 actgctggcg tagctggaga tccagcggcg ttttgctggg ttcgccgccg atttctcgcg
    60721 ccagtttcgg caccagataa cccgacacca gtgtcagcaa ctcacgcata atctgccgtg
    60781 cttcgtcatc actcaccata aaatgcgccg cgccctgtac tttatcgagc acatgcaggt
    60841 aatacggcat tacgccggca tcgaacaacg cattactcag gtttgccagc gtttttgcgt
    60901 tatcgttcac accacgtaac agaacgctct ggttaagcag ggtgacaccg acacggcgca
    60961 atttagccat cgcctgacgg aacgtttcat ctacctcatt ggcatgattg atgtgattca
    61021 ccagcaagat ttgcagcgta gaacgggcaa agcgttcaac cagcgcctcg gtgatacgtg
    61081 ccgggatcac aatcggcaga cggctgtgaa tccgcagacg tttgatatgc gggatggctt
    61141 ccagttgtgt gagcaaccag tccagctcgt gatctttcgc catcagcgga tcgccgccgg
    61201 agaaaatcat ctcgtccagt tccggatgag cagcaacata ctcaagagca gtctgccagt
    61261 tacgcttgtt gccctgattt tcggcatagg ggaagtggcg acggaagcaa taacggcaat
    61321 ttaccgcgca gccgcctttg accagcaaaa gcgcccggtt gtggtattta tgcaacaaac
    61381 caggcactac gctgtgctgt tcttccagtg ggtcggtgga gaatccgggc gcgacgacaa
    61441 actcatcttg cgaggtaagt acctgacgca aaagaggatc gtccggattg cctttctcca
    61501 tgcgatcgat aaatgagcgg ggcacacgca gggcaaacag ctttttggcg ctgcgtccgg
    61561 ccaacagttt ttcgtccgcg tctatattca aaagacgcag aagttcatca ggatcggtca
    61621 caacatcggc aagttgcgtt aaccaatctt ctctggatgg ggtatttagg gttacaatat
    61681 gcgccatttt gtggcttagc taccaattaa caaatttcag agggccttat ggcaacgtac
    61741 tatagcaacg attttcgtgc tggtcttaaa atcatgttag acggcgaacc ttacgcggtt
    61801 gaagcgagtg aattcgtaaa accgggtaaa ggccaggcat ttgctcgcgt taaactgcgt
    61861 cgtctgctga ccggtactcg cgtagaaaaa accttcaaat ctactgattc cgctgaaggc
    61921 gctgatgttg tcgatatgaa cctgacttac ctgtacaacg acggtgagtt ctggcacttc
    61981 atgaacaacg aaactttcga gcagctgtct gctgatgcaa aagcaattgg cgacaacgct
    62041 aaatggctgc tggatcaggc agagtgtatc gtaactctgt ggaatggtca gccgatctcc
    62101 gttactccgc cgaacttcgt tgaactggaa atcgttgata ccgatccggg cctgaaaggt
    62161 gataccgcag gtactggcgg caaaccggct accctgtcta ctggcgctgt ggttaaagtt
    62221 ccgctgttcg tacaaatcgg cgaagtcatc aaagtggata cccgctctgg tgaatacgtc
    62281 tctcgcgtga agtaatgcgg ttgtggtgcg gcctgcaggc tgcaccatca cttattcagg
    62341 tcagagatga tgaaacgcct tatcgttctt gttttgcttg ccagcacgct gctcacgggc
    62401 tgtaacaccg ctcgcggttt cggcgaagac atcaaacatc tcggcaactc catctcccgc
    62461 gctgccagct aatttttctt ctcttccgaa aaatcatcag atttccatca tttttggcga
    62521 tgttgtctat tattaatttg ctataggcaa acataaataa cattacctaa aaggaagacg
    62581 ttatggtgaa gaagacaatt gcagcgatct tttctgttct ggtgctttca acagtattaa
    62641 ctgcctgcaa caccacgcgt ggcgttggtg aagacatttc tgatggcggt aacgcgattt
    62701 ctggtgcagc aacgaaagcg cagcaataag caataacggt acgacagtta tgtcgtgccg
    62761 tttgtttttt tgcgatagtc acaaaggtaa tagttgaaat tcccctgcca cctggcaaaa
    62821 tatccgttca accatcagct ttgcaggacg acctgcaaac gcctcttttc accggggacg
    62881 gccccaattc tccggagcct gatatgtcct ggattatctt agttattgct ggtctgctgg
    62941 aagtggtatg ggccgttggc ctgaaatata cccacggctt tagccgtttg acgccgagtg
    63001 ttattaccgt gacggcgatg atagtcagta tggcgctact tgcctgggcg atgaaatcgt
    63061 taccagtagg gacggcttat gccgtgtgga cgggtattgg cgcagtcggc gcggctatca
    63121 ccggcattgt gctgctcggt gagtccgcta acccgatgcg cctggcgagt ctggcgttaa
    63181 tcgtattggg gattattggt ctgaaactca gcactcacta actaccgggc tgctgtaccc
    63241 aaataaactt actgacatca aacccttccc gggtcgcgac tgccagcatc tcctgtttca
    63301 cttcgtcaga aatggttggc gtgcgggaga gtatccacag gtagtcgcgg tccgggccgc
    63361 aaaccagcgc atggcggtat tcccgatcga gtgcaataac gttataaccg ccatagaaag
    63421 gaccaaagaa tgacactttc agcgcagcgc gagttggtgc gccggtaaag tacgctttcc
    63481 cttcactctg ctgccacatt cctctgtcag ggttatagcc tttattaatg acattcaggc
    63541 cgccgtcatc acgcaggctg tatgttgcgg tgactttttc cagtccacgt tcaaaacggt
    63601 gatcaaaacg ggcaatctca taccaggtac cgagataacg tttggcgtcg aaattattca
    63661 ctactgttac gccacgcggc ggcgtaggag aactgcaggc aacgaccaga aatgcagctg
    63721 tcgctgcggc aacgagaggg agcaggcgca taaatgtttc cttactggtt tttttctaag
    63781 tgtagatgac agcaaggaaa agcgcagaaa aggtccgaaa attcggaccc gatgaaattt
    63841 tactgtagag cgttgagaat ctgccaggcg gcggcgactc tcgctggatt gggatagttt
    63901 ttgttagcca gcatcactat acccagctct ttttctggaa taaacgcgac atagctacca
    63961 aatccgccgg ttgcccctgt tttatgtacc catgatgcgc gtactgcagg agttgggggc
    64021 gtaatcgctt ttacggggtg tgctgccagt gcaattttat tgccactgcc gttaatgatg
    64081 ctgtcaggat ttaccggcca gtccagcatt tcccagccca ggccctgata catatcgccg
    64141 gtttgccagt agcgagattg tgccagttgt atcccttgct gaagtgtttt gtcgttgata
    64201 tcacggggat tcatattgct tcgtacccag caggccatat cttcaatggt cgacttcaca
    64261 ccataagttt tagcatctaa cgcccctggc gaaacatgaa ctgccttgcc ttcgcgatat
    64321 ccccaggcgt aattcttttc ttctgcgggc ggtacattaa tccacgtatg gttgagtttg
    64381 agtggctgga agacacgtgt ttgcatcgcc tgctcaaaac tcaaaccaga cggcttcaca
    64441 gccagtgcgc cgaacaaacc gatactggag ttggcataca gacgttgtgt tcctggcgcc
    64501 catgcaggct gccagttttg atagaagcgc agcaagtcgc ttgaggattt cacctcatcc
    64561 ggcacctgca atggcaggcc gccagcagtg taggttgcga gatgtaatag tgtgatccca
    64621 ttccactgtt tagcggtaag ttcaggccag tattttgttg tgggatcgct taacttgatt
    64681 tcccctcgag caatagcgtc gccaccaagc acgcccgtaa atgttttgct gaccgaacct
    64741 aactcaaaca acgtttgctg tgtgacgggc tgctttttgg cgatgtccgc atagccccag
    64801 gtaaagtaat aaggtttacc ctgataaatt accgccaccg ccataccggg gatcttttgt
    64861 tgctctataa gcggggtaat tgtgcgatgc acaatatcgt tgatttgttg aggggcagca
    64921 aatgtggagc aagaggcggt aattaataag gcgcagagcg tcgttttgaa cataaggtct
    64981 ggtttccata caaaacggcc cgccataggc gggccggatt tacattggcg atacgttaga
    65041 ttgtaacgat accaatcagc gtgacaactg tcaggatagc agccagaccg tagaaaaccc
    65101 atttgcccgc aggcacgtga attttcagat cgtgcatcgc gtggtgcata cggtgtaaac
    65161 cacaccacag cggcagaacg atcatcagga acaggaatac gcgaccaatg aagctctgcg
    65221 cgaacgccag aacgcgctcg tagctcagcg catcacccgg aaacagcccc agtggcagca
    65281 gaatacccac cagcaggatc atcaccggcg caataattgc gctccacata ccaccggccc
    65341 cgaagaggcc ccagaatacc ggttcgtcag aacgctttgg atttggatta atcatctcag
    65401 gctccttacc agtacagggc aacaaacagg attacgatgg tggcaaccac agttaccgcc
    65461 cagagacttt tgataattgg ctctggtccc attttttcgt cttttacaat gatattggcc
    65521 gctttcggtg ccagttcaaa ccaggttttg gtgtgcagca gagctgccgc cagagtgatc
    65581 aggttaatga tcacgataac cgggttttgt aaaaagtcga cgaatcccgc ccaggcttcc
    65641 gggccatttt tcagggcaaa cagcccgaaa atcagttcaa tgctgaacca cacagccgga
    65701 accgccgtgc cttcgcgcag catgtaaaag cgataaaacg gcaatttttt ccaccaggtg
    65761 gacgtcattg gccgtacata cggtttacgt ttagtcgtca tgttgcactc cttagcgtgg
    65821 tttcagggtc gcgataagaa agtctttcga actttctact ttgccctgct gaatggccgc
    65881 agccggatcg acgtgtttcg ggcagacttc ggagcaatag cccacgaaag tacagctcca
    65941 tacgccgttc tggctgttca actgcgccat acgctccttc ttaccgtggt cgcggctatc
    66001 ttcgttataa cgatgcgcca gcgtaatggc agccggaccg atgaactctg ggttcaggcc
    66061 aaactgcggg cacgcggcgt agcacagacc acagttgatg caaccggaga actggtgata
    66121 ctttgccatc tgcgccgggg tctggatgtt agtaccctga tccgcggtgc gggagttgcc
    66181 gatgatgtac ggtttgatcg cttccagact ttcgatgaag tgggtcatat cgaccaccag
    66241 atcgcgttca atcgggaagt tagctaacgc ttcaaccttc ataccgtcgg tgtaatcacg
    66301 caggaaggtt ttacatgcca gttttggcac gttgttaacc atcatgccgc aggaaccaca
    66361 aatcgccata cggcaggacc agcggtaact caggtccggt gccaggttgt ctttgatgta
    66421 gcccagcgca tccagtaatg aggtagttgc gtcataaggc acttcataga atgcgctatg
    66481 cggtgcggta tcgacttccg ggttatagcg caccacctca attttcaggt ttttcatctc
    66541 agccattcgc cttctccttc ttattggctg cttccgcctt atcggctgca tccgcttcgc
    66601 caccgtaaac gcgtttagct ggcggcagcg tagtaatctt cacgtcgctg tactccaggc
    66661 gagtcgtgcc atcagcatcg cggaaggcga gggtgtgttt gaggaagttg acgtcgtcac
    66721 gctcggtgca accttcgtcc agacgctggt gtgcgccgcg ggactcttta cgtgccattg
    66781 cggagtgcgc catacattca gcaacgttca gaccgtggcc cagttcaatg gtgtagagca
    66841 ggtcggtgtt gaacacgctg gaggtgtcgg tgatgcgcac gcgcttgaag cgttcctgca
    66901 gctctgccag cttgtcgatg gttttctgca tcagttccgg cgtacggtag ataccgcaac
    66961 cttcttccat tgccaggccc atttcgtcgc ggatcttcgc ccagttttcg ccgccatcct
    67021 ggttaaccag atctttcaga cgttgttcaa cgccagctgc ctgcgcttca attgccgctt
    67081 cgttgccatt accggcagtt gctgcacgct ctgtcgcttg ttcaccggcc agacggccga
    67141 agaccaccag ttccgccagg gagttagaac ccagacggtt tgcaccgtgc agaccaacag
    67201 aggaacattc acccacggcg aacagacctt taatgcgggt ttcacagttc tgatcggttt
    67261 cgataccgcc catggtgtag tgtgcggtcg gacgtaccgg aatcggttct ttaaccggat
    67321 cgacgccaac gtacgctttc gccagttcgc agatgaacgg cagacgttca tgcagttttt
    67381 tctcgccgag gtgacgcagg tcgagataaa ccacatcgcc acgcggcgtg gagatggtgt
    67441 tgcctttacg ccattcgtgc cagaaggcct gagaaacttt gtcgcgtgga cccagttcca
    67501 tatatttgtt tttcggctcg cccagcggag tttccgggcc catgccgtaa tcttgcagat
    67561 aacggtagcc atttttgttg accagaatac cgccttcacc gcggcagcct tcggtcatca
    67621 ggataccgga acctggcaga ccggttgggt gatactgaac gaattccatg tcacgcagcg
    67681 gaacgccgtg gcttagcgcc atacccatac cgtcaccggt aacgatgccg ccgttggtgt
    67741 tgtaacgata aacgcgaccc gcaccgccag tagccataac gaccgcgtta gcacggatct
    67801 gcaccagcgt gccttccatc atgttcattg ctaccaggcc gcgaacatga ccatcatcaa
    67861 ccagaatatc cagcacgaaa tgttcgtcaa aacgctggat ctgcgggaat tgcagagagg
    67921 tctggaacag cgtgtgcagc atatggaagc cggtcttatc ggcggcgaac caagtgcgct
    67981 cgattttcat gccgccgaag cgacgtacgt tgacgctacc atccgggcga cggctccatg
    68041 ggcaccccca cagttccagt tgggtcattt cggttgggca gtggtggacg aaataatcca
    68101 cgacatcctg ctcacacaac cagtcgccac ccgctactgt atcgtgaaag tgatattcga
    68161 agctgtcatg atcctgcgcg acagcggcgg agcccccttc tgcagcaacg gtatggctac
    68221 gcatcgggta tacttttgag attagtgcga tttttgcatt tggatttgcc tgcgcggcag
    68281 caattgcagc acgtaatccc gcgccaccgg cgcctacaat ggcaagatcg gcttgaaagg
    68341 tttgcacgac attcctccag attgttttta tcccacagcc ccgtacttca gggtaagtgc
    68401 ctgaaagtta cggtctgcga acgctattcc actgctcctt tataggtaca acagtatagt
    68461 ctgatggata agtctgaaat ttgacgagat cgattttttt agtgcgcaag agggctaaat
    68521 tatcactgaa gatgattaat ttaattacta aaccatcaga tcgtgctttt tttagtcact
    68581 gcccaccgct gtttgattcc tgcgtaaaat ttgtctcgcc gtcgcgttgc gagtagactt
    68641 cgtgcccttg tcaaaaactg gagatttaac tatgagcgaa acggcatcct ggcagccgag
    68701 cgcatccatt cctaacttat taaaacgcgc ggcgattatg gcggagatcc gtcgtttctt
    68761 tgccgatcgt ggagtgctgg aggtggagac gccttgtatg agccaggcga cggtaaccga
    68821 tattcatttg gtcccgtttg agacacgttt cgttggcccc gggcattcgc aggggatgaa
    68881 tctctggtta atgaccagcc cggaatacca tatgaaacgc ctgctggttg ccggttgtgg
    68941 gccggtattc cagctgtgcc gcagcttccg taatgaagag atggggcgtt atcacaaccc
    69001 tgagttcact atgctggagt ggtatcgacc gcactatgat atgtaccggt tgatgaacga
    69061 ggtggacgat ctcttacaac aggtgctgga ctgcccggca gcagaaagcc tttcttatca
    69121 acaagctttc ttgcgttatc tggaaattga cccgctctct gccgacaaaa cgcaactgcg
    69181 ggaagtcgca gcgaaactgg atttgagcaa tgttgctgat accgaagaag accgcgacac
    69241 gctgctacaa ttgctgttta cctttggcgt agagccaaat attggcaaag aaaaaccgac
    69301 ctttgtgtac cactttccag ccagccaggc atcactggcg caaatcagta ccgaagatca
    69361 tcgggtcgct gaacgctttg aggtttatta taaaggtatt gagctggcga atggtttcca
    69421 tgaattgacg gatgcccgtg agcagcaaca acgctttgaa caagataacc gtaagcgcgc
    69481 ggcgcgcggt ttgccgcagc accccattga ccagaatctg attgaagcct tgaaagtcgg
    69541 tatgcctgac tgttccggcg tggcattagg tgttgatcgt ctggtgatgt tggcgctggg
    69601 cgcggagaca ctggctgaag tcatcgcctt tagcgttgac cgggcataat tctgaaaaat
    69661 tactgaatta acagcgaaga atggcgtgat aagcgccatt ctgtagcata tgttttcatt
    69721 tttccgttaa aggccacaat ttaagactgg atatttaata tcatccaggt atcaattctg
    69781 tattggtttt tacgctgcct gctcaatatt gcgcagtgca gttcagtggg cacgtgttat
    69841 acacgcgctg aaatgaagga tggtttcatg cctcacacga taaaaaagat gagtctgata
    69901 ggactcatat tgatgatctt tacttccgta tttggatttg ccaatagccc atcggcttat
    69961 tacttaatgg gttatagtgc gattcccttt tatatatttt ctgcattgtt attctttatt
    70021 ccattcgcct taatgatggc tgaaatggga gctgcttatc gcaaagaaga aggcggtatc
    70081 tattcctgga tgaataatag tgtcggacca cgttttgcct tcattggtac gtttatgtgg
    70141 ttttcctctt atatcatctg gatggtgagt acctccgcga aagtttgggt accgttctca
    70201 acattcctct atggtagcga catgacccag cactggcgta ttgctggact ggagcctacg
    70261 caggtggttg gtctgctggc agtggcatgg atgattctgg tcaccgtcgt tgcttcaaag
    70321 gggattaata aaattgcccg cattactgcg gtgggcggta ttgcagtaat gtgtctgaat
    70381 ttagtattgc tgttagtaag cattactatt ttgttattaa atggtgggca tttcgcgcag
    70441 gatattaatt tccttgcatc accgaaccca ggttatcagt ccggtctggc aatgctatcg
    70501 tttgtggtat ttgctatttt tgcctatggc ggaattgaag cggttggtgg tctggtcgat
    70561 aaaacggaaa atccagaaaa gaactttgcc aaaggtattg tttttgccgc tattgttatt
    70621 tcaatcggtt attcgctggc aatattttta tggggtgtca gcacaaactg gcagcaggta
    70681 ttaagtaatg gttccgttaa cctcggcaat attacctatg tgctgatgaa gagcctcggg
    70741 gtgacgctgg gtaacgcaca gcatttgtca cctgaagcgt cattgtcgct gggtgtatgg
    70801 tttgcgcgta ttaccggact ttcgatgttc ctcgcttata ccggggcgtt ctttacgctt
    70861 tgctattcac cgctgaaagc catcatccag gggacgccga aagcgttgtg gccggaaccg
    70921 atgacgcgcc tgaatgcgat ggggatgcct tctatcgcca tgtggatgca gtgcgggttg
    70981 gttactgtct tcatcctgct ggtttcgttt ggtggcggta ccgcatcggc gttctttaac
    71041 aagctgacgc tgatggcgaa cgtgtctatg acgcttcctt acctgttcct cgcgctggct
    71101 ttcccgttct ttaaagcacg tcaggatctc gacagaccgt ttgtgatttt caaaacgcat
    71161 ttgtcggcaa tgattgcgac agtggttgtc gtactggtgg tgacatttgc gaacgtcttc
    71221 accatcattc aacctgtggt tgaagccgga gactgggaca gcacattgtg gatgattggc
    71281 ggccctgtct tcttctcgct gttagcgatg gcgatttacc agaactattg cagcagagtg
    71341 gcaaaaaatc cgcagtgggc ggtggaataa cccgatgccc tatcgtctgc ctgacgatag
    71401 ggcgcaatga caggaaatgt tatggatgac accagccggg acccggcaat aaccgaggat
    71461 gaaatcaggg cgctgcaatt ttctgccggg gatgtagcgg aaatagagca gactattctc
    71521 tcttttgtcg atgcctgcca cacccgtaaa gtggcgatgg tggtgggtag caccattaac
    71581 accctcaaag atcgcgatgg taaacggtgg ggaaacctgc tggatattta ttgcgcttat
    71641 cttatccgat gtctggtttt tcgcggggaa ttagtgggat acggcgattt gtttcatatg
    71701 cgctatagcg aaattaagcg accggttacc ttatgagtgc gcgcatgttt gtcttgtgct
    71761 gcatctggtt cattgtggcg ttcctctgga taaccattac ctccgcgctg gacaaagagt
    71821 ggatgattga tggacgcggg attaacaacg tctgcgatgt gttgatgtat ctcgaagaag
    71881 acgatatccg cgatgtcggt gtgataatga cgttaccgct ctttttccca tttctctggt
    71941 tcgcgctgtg gcgaaaaaaa cgcggctggt ttatgtacgc caccgcgctg gctattttcg
    72001 gctactggct gtggcagttt tttctgcgct atcagttttg tttgtgagcc ggatgggttc
    72061 atccggccac aaagacctta caaacttccc gcctgacgac ctttgcccgc agaagttaat
    72121 gttctccccg tttgcttacc attcaggctt tccagacgca tctggaacgg tgggaatggc
    72181 atgtcgatac cgtgggcgtg gaagcctgcc agaattaact ggtggatctc atggcgcagc
    72241 ggcatacggt gacccatctc agcggcgtaa atacgcagct cgaaaatctg aatcccctgt
    72301 tgcagatcca ccaggaagac ttccggtgcc gggttgtcga tcaccagcga gcagcgacgc
    72361 gctgcggtga gcaggatttc cgtcacttct tcactgttgg catcggcagg ggccggaatc
    72421 gtcaacacca cgcgcgtaac cgagtcagag agcgaccagt tgataaactg ctcggtaata
    72481 aacgccttgt tcggcacgat tatctcttta cggtcccagt cgctgatggt ggtggcgcgg
    72541 gtgttaattt tcgtcacgct accggtgaga tcgcgaattg tcaccgtatc gccaatgcga
    72601 atcggttttt cgaacaggat aatcagacca gagataaagt tggcgaaaat ttcctgcaaa
    72661 ccaaaaccga gaccaacacc gagcgcggcg accagccact gcaatttcga ccactcaata
    72721 ccaatcatcg agaagccgac cagcccgcca atcagcatca gcagatattt ggtgatggtg
    72781 gtgatggcgt agcccgtacc cggcgttaaa tccaggtgct gcaaaatcgc cagttccagc
    72841 agcgcgggca ggttgcgcac cagctgcgtg gtgatgataa acaccagaat ggcaatcagc
    72901 accgcaccga gggtaattgg ctccagactt tctacgccct gtaccgtgga ggtgacatcc
    72961 cacagcgaaa tattttcgag gaagccgaaa gcggaatgga tttctgacca cagcacaatg
    73021 acagaaagca gggcgatcaa catcaaaatt gagcgcacca gccgcaagga ttgcgcactg
    73081 atggcatcga gatcgacttc gctttcatcg acttcaattg ccccttccgg gctactgtga
    73141 tgatgcgctt cctcttcgcc acgcgcgcgt tgcgctaaca tctctgcccg gcgatgcttc
    73201 gcccggtcaa acgccagcct gcgacgctgg atcagcatcc agcggcggat aacgtgataa
    73261 accaccagta gcaggaacca gatggcaacc gaggtttcaa gcctcgctaa cagtgcctgc
    73321 gccgttgcca gataacccac cgctgacgcc agaatggcaa ccagtggtgc gccaatcatc
    73381 atgttccaca gcatatggtt ggtaatgttg tcgccgctgc cctctttgtt gaggtacagc
    73441 gggatcccgg cctttttcag gctgagggtg accaccgcca gcgcaccgca aatgaggata
    73501 aagcaaagcc gtcccagcga accggagaat tcacggtcgt cgaggttatc gaacatcatc
    73561 agcgccataa tcagcggcac aataagcccg atgctcatca ggtagtagcg catcccacgg
    73621 gaaacacgtt cgcgcggcca gccaaaatga gcgataaaca agccgttcgg gcgggcaaag
    73681 gtggcgcaaa tcatcactac ccacagcagc ggcacggtgg ccgttacgcc atcgccaatc
    73741 gcgaccgcca gcggataagg ccacgcctcg cgcaagccgt aacccagcgt catccacagc
    73801 accggcagcg gtgacgcgac gagaatcgac cagaaaagag tgcgcaacgt cagccagaag
    73861 tgatcctgag tcactttgcc gactttcgcc gccgaacgtt caagaaaacg ggtgaaatag
    73921 cggcgcgagt aaatactgca accgaccaga atcaacgcgc caaacagcgg caaaatcgtc
    73981 tctttgctgg tcagcatcat cacactggct ttgcccaact gactgaaggt gtccagcgaa
    74041 atgagacgac gcagatcctg ggcgatttcc agcggccagg cgatggtcat cgggcgcacg
    74101 tcagaggtcc agaacagata gcggtgcgtt gcttcgttca cctctttcag cgcatcctcc
    74161 agttgcccgt tggagacttt cagcttggtc agttccagta gtagcgtgtc gccaccctgc
    74221 aacaatgagt tcagcaactc acgctgagtg cgcagttgtg cttccagaat acggttttgc
    74281 tcggcggtca gcggctgacc gtcggcctga tggatttgcc gtagcagcgg ctgtttatta
    74341 agcagatcct cataacgtaa ccgttgcaca cgcaactgcg ccatttcggt atcaagctgt
    74401 tgtggttttg gcatttctgg tagccgtgcc acctgcgccc gcagcgcttc accgagcagg
    74461 ttggacgatc ccagccattg cgactgttca cgcagcgtat tcaacgcctg ccggacctgt
    74521 aacgtctggc tggcagcctg acgctgttgc gaggcaacga gatccatccg ctgcgcctgt
    74581 tgattcagtg ccgccgatag ttcgcggtta attttgaatt gcgcgacgat atctttcggc
    74641 aaatcggcac tgttttctgc cagtaattcg gtactttcca gcgcccgctc cgcctcaagc
    74701 tgacgttggc tattcaactg attacgtaag gcctgcaaat acgcatccag ttgctggctc
    74761 tctttttccg ccagctctga gcgcaagcgc gctaattcct gacggttatt ggcagacagc
    74821 tgcgccagtt ccagttcatc aacgagcgct ttcaggcgtg cggagtcaga ctgcaacgcg
    74881 aaattttgtg cctgattgag cggagtattg ccggtaagcg ttcccaggcg gcgctcgatc
    74941 tcatttaact gacggcgggc atcggtttgc tgttgcggca gttgattcag tgaatcggca
    75001 atctcgcggg cgcgctcctg ctcttgctgg gcctgacggc tcttatccag caactgactg
    75061 ctgacctgga gaatttcctg attcagcgca tcggtagaca ttcccggcga cacgctgcgc
    75121 ggctcgtcac gcatgttgtt taattgtgcg cgcagagtag cggagagttt cgggtaatta
    75181 tcgataactt gctgatattg tttgatgcgc tcaagggaac cttttcgttc ctcaagcgca
    75241 tttaaggcag actggagtgc ctctatgact tccggctgcg cgggtttcgc cgcttttgcc
    75301 tgctccagtt cctgggtgat ttgtttgcta tcgggggccg tcgcggcgta cgccccccaa
    75361 ctgaggcacc aggccatcag aaaagtgata atcaggcgca cgtcagcgtt tcctttgatg
    75421 gattagacct ggtctttttt gtcgtcaacc aatgggctgg cgtcgtgttc tgcttcgatc
    75481 tcttcagcag gaagcggggc aggttcagcg tctggcgtaa caaaggtttc ggtagatact
    75541 gccagcggct ggccaatttt cgtgacagac aggctttcca gttgctcaac cagattcact
    75601 ttacccggtg caaacaggtt gataacggtg gaaccgagtt taaagcgacc catttcctgg
    75661 cctttaagca gtgccacaga accgtcgttt tccccggcag gccaggtcca gcgcttgatg
    75721 ataccttcgc gcggcggcgt aatggtgccc gcccagaccg tctcaatgct gccaacaatc
    75781 gtcgctccga ccagaatctg cgccataggg ccaaattcgg tatcgaaaag gcaaatcacg
    75841 cgttcgttac gggcaaacag attcggcacg ttctgagcgg tgagatggtt aacggagaag
    75901 agatcgcccg gcacgtagat catctcacgc agaataccgt tgcacggcat gtgtacgcgg
    75961 tggtagtcac gcggggagag gtaagtggtc acaaacgtac cgttgcggaa caggttcgcc
    76021 atcagatagt tgcctgccag caaggcttcg aggctgtagt tgtggccttt ggcttgcagg
    76081 attttatctt cttcgatttt acccaactgg ctgataacgc catcggcagg catgaccagg
    76141 acattcggat cggtatcgat tgggcgtact tcgtcacgca gcggacggac aaagaattcg
    76201 ttaaaggtgc ggtagctggc ggtgtccggc ttttgcgcct ctttcatgtc gaccttgtag
    76261 tatttaacga acagatcgat aaccagtttt gtcagccatc ctgcccgctt gcttgcgccc
    76321 caacccgcca ggcgagtaag ccatagtttc ggcagaatgt actgtagcga aagtttaaat
    76381 gaatttaaca aggtagcctc caggccattg ttttgtcgtt cctgatccgg ccaacatgcc
    76441 ggatcctgaa aaaaagggga cgattctaac gacggttagc ttaattgtca gtcatccgta
    76501 tcagaaaagt ttttacgcgt ttttacctgc gccatgcttt ccagaatacg gtgatagttt
    76561 tcgaaacggg tttccgcgat tttcccttcc tcaaccgctt cccggatagc gcagcccgga
    76621 tcggtatcgt gtttgcaatc gcgatattta cacagaccta aatagtcatg gaattcgaca
    76681 aagccctgag tgatttgttc cggctccagg tgccagaggc cgaactcacg cacaccaggg
    76741 gaatcaatca catcaccgcc gtgcgggaag tgatacagcc gagcggcggt cgtggtgtgc
    76801 tgaccgagcc ccgagttgtc agagacatcg ttggtcagga tctctttttg cagccccagc
    76861 aacgcattca gcaggctgga tttgccgacg ccagactgcc cggcaaaaat gctgatgcgc
    76921 ccggtcaacg cctcttccag cggttttagc ccatcctgag tatggctgga aaccatcaat
    76981 acgcgataac caatgttgcg gtagatatcc atctgctcgt tgacgaacgc catgccttcg
    77041 tcgtccagca ggtctatctt attgagcaca ataatcggct caatctgcaa ggtttcgcag
    77101 gccaccaggt aacggtcgat aatattgagc gacagctccg gcaaaatggc ggagacaatg
    77161 acaatctggt cgatgttggc ggcaataggt ttcacgccgt cgtagaaatc cggacgcgtc
    77221 aacactgagg tgcgctcatg caccgcttcc acgatccctt tgacatttac gccttccgcc
    77281 gccggtttac ccggacgcca gactacgcgg tcgccggtta ccagcgaacg gatggtacgg
    77341 cgaatattgc agcggtgaac gtcgccatcg gcggattcca catcagcgtg cataccaaag
    77401 cggctgatga cgataccttc atcaggctcg ccaaacagat tgtcgtcgta gtcgggcttc
    77461 tccttagacg ttttaagacg acgctggtga ttggcgttca cgcggcgctg ctggcctttg
    77521 gagagtttat ttttactcaa tcgtacagac tcctggtcgc ccctgatggg caaaacatct
    77581 atgatacacg caattgtgga tcaatatagt cactgtgaat gggtggaaaa tagcatgagt
    77641 gccaatgaaa acaacctgat ttggatcgat cttgagatga ccggtctgga tcccgagcgc
    77701 gatcgcatta ttgagattgc cacgctggtg accgatgcca acctgaatat tctggcagaa
    77761 gggccgacca ttgcagtaca ccagtctgat gaacagctgg cgctgatgga tgactggaac
    77821 gtgcgcaccc ataccgccag cgggctggta gagcgcgtga aagcgagcac gatgggcgat
    77881 cgggaagctg aactggcaac gctcgaattt ttaaaacagt gggtgcctgc gggaaaatcg
    77941 ccgatttgcg gtaacagcat cggtcaggac cgtcgtttcc tgtttaaata catgccggag
    78001 ctggaagcct acttccacta ccgttatctc gatgtcagca ccctgaaaga gctggcgcgc
    78061 cgctggaagc cggaaattct ggatggtttt accaagcagg ggacgcatca ggcgatggat
    78121 gatatccgtg aatcggtggc ggagctggct tactaccgcg agcattttat caagctgtaa
    78181 aatttttcct ggtcacgtaa gcgccggaat ccacttgcgg cggcgtgatt ttgacgctaa
    78241 attgaacgtc ttgctgataa tttcagcact tgagataaaa acgcaaaaaa aacttttttg
    78301 gggggttgca gagggaaaga tttctcgtat aatgcgcctc ccgtaacgac gcagaaatgc
    78361 gaaaattacg aaagcaaaat taagtagtac gcgggaatag ctcagttggt agagc
//
LOCUS       DAZFQD010000004        74623 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.004, whole genome shotgun sequence.
ACCESSION   DAZFQD010000004 DAZFQD010000000
VERSION     DAZFQD010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 74623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 74623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..74623
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.004"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..231)
                     /locus_tag="ACHKTW_000270"
     CDS             complement(<1..231)
                     /locus_tag="ACHKTW_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SelA-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HFO6461363.1"
                     /translation="MTQNIYQQLGLKQVINACGKMTILGVSSVAPEVMQATARAASSF
                     VEIDQLVDKTGELVSRFTGAEDSYVTSCASAGI"
     gene            complement(235..1095)
                     /locus_tag="ACHKTW_000271"
     CDS             complement(235..1095)
                     /locus_tag="ACHKTW_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462668.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFO6461364.1"
                     /translation="MMSDDVMQHELVERARESGTLTKADITKAWFIYWLGAEVSSSYE
                     RLQSLIFCASMTPIIKKLYPQKEEQVEALKRHLNFFNSEQTFGAVIQGISIAMEEQKT
                     RGEEINDASITGIKTGLMGPLAGMGDSIIWAAVMPLLIAIFIPFAANGSAMGGILPLI
                     LYPAITLAISYGMVHKGYTLGRDSIISLLQGGRIKELIYGANVLGLIMMGALSASFVK
                     ITSPLKISALKGSEVVVQQILDSIAPGLLPLAAVFAIYFYLVKKGPRYTTILLSIVAL
                     SVVCSLLGVL"
     gene            complement(1092..1841)
                     /locus_tag="ACHKTW_000272"
     CDS             complement(1092..1841)
                     /locus_tag="ACHKTW_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFO6461365.1"
                     /translation="MDTLLFASLMGLYYWFARLRLGYTFSAMLLQPVVVAVFVGLLLG
                     NMPAAMIIGAGMQLVYLGVTSTPGGNVPSDPALAACIAIPIAVKANMDPNLAIALAIP
                     FGVIGVFLDQLRRTLNAAWVHMADKHAETANMSAIMRCAFLYPALLGLVLRFPVVFAA
                     NYFGQNVVESFLKLMPHWLTHSFEIMGGILPALGFAITIMVIGKKSLLPWFIGGFFAV
                     LYLKVDIMAMAIFGTCVAFLIKGLAKNEGAA"
     gene            complement(1867..2352)
                     /locus_tag="ACHKTW_000273"
     CDS             complement(1867..2352)
                     /locus_tag="ACHKTW_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFO6461366.1"
                     /translation="MANIVLCRIDSRLIHGQVVTKWVGQSQANRIAVVSDELDADPFM
                     KNIYLMAAPPNIKVDCFSNQSFAAAWKENQLGDGNVLVLFPSLAAIQEAVQQGFDVAD
                     IQVGGLGGGPNRKAVFQNITLDEKDVGILNDLKSRGIKAVFQTIPEDKPQSLDDILKK
                     F"
     gene            complement(2363..2791)
                     /locus_tag="ACHKTW_000274"
     CDS             complement(2363..2791)
                     /locus_tag="ACHKTW_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFO6461367.1"
                     /translation="MTTTVPIHPLPEILLLTHGGWGLQLCNSLRMVMGEIEGVTEIAL
                     MPVDTLGEFYQRVEAVVKTMPEGSLIVTDFIGGTTSNVAARLSADYPVAVISGLNASL
                     LLEALDKREHGPLTACVADLVEAGRNSCLDVVSHIRQLQQ"
     gene            complement(2910..5708)
                     /gene="dagR"
                     /locus_tag="ACHKTW_000275"
     CDS             complement(2910..5708)
                     /gene="dagR"
                     /locus_tag="ACHKTW_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator DagR"
                     /protein_id="HFO6461368.1"
                     /translation="MRRIDVIHKELERLTYGLELSDLAKEKAFTAEAIGFNLGLARNS
                     VSKDLNQLWNEGLVVKSQGRPVFFLHRHALELLINRKLDDCECIVHSVASLLPKKEKY
                     TDDDPFSGLIGYDRSLRDAVEKGRAAVLYPHGLHVLLTGASGVGKTFFAELMHCFACK
                     HTIGSPPPLVYFNCAEYAHNPELLSSHLFGHHKGAFTGASENKTGLVEQADGGYLLLD
                     EVHRLPYEGQEKLFSILDKGEYRPLGSSGPTYSISVRLICATTESVNSALLRTFQRRI
                     QVCIDLPGIRQRSVEEQLELIVSFFQRESRKIERTISLDKTLLHWLLSKPPEGNIGQL
                     KSDIQFLCAQAWASGMTERNDTLELDKRLAEMSFNTTPEQRLLVDALFGGKDRLNVDA
                     RTLPALKNSLATSAEIEESDLFYSFLTREYVNLRNSNVPPAETLAILKNKLSSIFEYG
                     LYSRHSAAHPPRYGDQIEERVTLLIGYVEQVLGFTLPENLANPLRKHFLALIGYVRRG
                     LIPQLYSSSLILDRCKDEYDNATLLCRKINELLHIQCPATEVVWLCLFLKECRHYRQR
                     IDTSPDCGVILIAHGATTATSQAQYVNRVLERDLFYAIDMPFEQSVHDTLETLTRMIQ
                     DRCWQRLILMVDIGSLVHFGSTISKLFQIDVLLLPNITLTSLLEVGLDLSYETGDLSQ
                     LAVLMQSKNIPCRLCTPQQESGGKVLVISCITGMGTAEKIKKVLEESFGELMSQDTRM
                     VILDYNEVRSLERIQQALNASERLAGIVGTFQPGLPDIPFISLEELFSEQGPELVLSL
                     LTPDLSSSERRLEMERSAMRFISALTMESIINHISVLNPQRILKEIEDVLNYLTNTLS
                     LKPSRQVTLRFLIHCCCMVERIVINRKPLQMALENRLDLDARAFSVIKSSFLPIEEAY
                     AIRLSDAEYFYIYELLYS"
     gene            complement(5967..6887)
                     /gene="speB"
                     /locus_tag="ACHKTW_000276"
     CDS             complement(5967..6887)
                     /gene="speB"
                     /locus_tag="ACHKTW_000276"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HFO6461369.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(7023..7754)
                     /locus_tag="ACHKTW_000277"
     CDS             complement(7023..7754)
                     /locus_tag="ACHKTW_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6461370.1"
                     /translation="MKKWKVRSALVALIVLLAGCSSNAQYNSSASGNVGTAWGGDVHS
                     TVQGVSAERAWRDPAEMIVISYSTNVPSGYDRVYSIRINELEYAIRDGNFNSLPITRV
                     YDSSNNEPRYIVHARVGMNYQLYVRNYSRNTNYEIVATVDGLDVLNGKQGSLNNNGYI
                     VNAGDSLAIKGFRKDKHTEAAFQFANVADSYAANSAQGDVRNTGVIGFAAFELQGPAQ
                     NALPPCSGQAFPADNNGYAPPPCRK"
     gene            complement(7900..9876)
                     /gene="speA"
                     /locus_tag="ACHKTW_000278"
     CDS             complement(7900..9876)
                     /gene="speA"
                     /locus_tag="ACHKTW_000278"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HFO6461371.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPAE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            complement(9885..10016)
                     /gene="yqgB"
                     /locus_tag="ACHKTW_000279"
     CDS             complement(9885..10016)
                     /gene="yqgB"
                     /locus_tag="ACHKTW_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HFO6461372.1"
                     /translation="MKKKPVAQLERQHSLLENPCAYGLLSQFQAAIVVNCFTLNKII"
     gene            complement(10110..10253)
                     /gene="yqgG"
                     /locus_tag="ACHKTW_000280"
     CDS             complement(10110..10253)
                     /gene="yqgG"
                     /locus_tag="ACHKTW_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgG"
                     /protein_id="HFO6461373.1"
                     /translation="MNRCLLLNLSHRSGEDSFPALCISALHTCRCYTHLGASQDSRAG
                     YSY"
     gene            complement(10364..10615)
                     /gene="yqgD"
                     /locus_tag="ACHKTW_000281"
     CDS             complement(10364..10615)
                     /gene="yqgD"
                     /locus_tag="ACHKTW_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgD"
                     /protein_id="HFO6461374.1"
                     /translation="MVADPTTTLQVKNTGSLSVNRYGWINIWMAILGQFFTRFPLFFE
                     SCLILLKTWLEIFPDNAGILRIYLLQFSAIVGYKTRRAA"
     gene            10671..11825
                     /gene="metK"
                     /locus_tag="ACHKTW_000282"
     CDS             10671..11825
                     /gene="metK"
                     /locus_tag="ACHKTW_000282"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFO6461375.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
     gene            12261..13655
                     /gene="galP"
                     /locus_tag="ACHKTW_000283"
     CDS             12261..13655
                     /gene="galP"
                     /locus_tag="ACHKTW_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HFO6461376.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGSGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            13732..14229
                     /gene="yggI"
                     /locus_tag="ACHKTW_000284"
     CDS             13732..14229
                     /gene="yggI"
                     /locus_tag="ACHKTW_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HFO6461377.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAVYRCVHCGE
                     QLVAK"
     gene            14324..15031
                     /gene="endA"
                     /locus_tag="ACHKTW_000285"
     CDS             14324..15031
                     /gene="endA"
                     /locus_tag="ACHKTW_000285"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HFO6461378.1"
                     /translation="MYRYLSIAAVVLSAAFSGPALAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            15111..15842
                     /gene="rsmE"
                     /locus_tag="ACHKTW_000286"
     CDS             15111..15842
                     /gene="rsmE"
                     /locus_tag="ACHKTW_000286"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311849.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFO6461379.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGKIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            15855..16805
                     /gene="gshB"
                     /locus_tag="ACHKTW_000287"
     CDS             15855..16805
                     /gene="gshB"
                     /locus_tag="ACHKTW_000287"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HFO6461380.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTRYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            16914..17477
                     /gene="yqgE"
                     /locus_tag="ACHKTW_000288"
     CDS             16914..17477
                     /gene="yqgE"
                     /locus_tag="ACHKTW_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417423.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HFO6461381.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            17477..17893
                     /gene="ruvX"
                     /locus_tag="ACHKTW_000289"
     CDS             17477..17893
                     /gene="ruvX"
                     /locus_tag="ACHKTW_000289"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFO6461382.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(18069..19049)
                     /gene="yggR"
                     /locus_tag="ACHKTW_000290"
     CDS             complement(18069..19049)
                     /gene="yggR"
                     /locus_tag="ACHKTW_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HFO6461383.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGLMEAAPFDAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRQGISLALRLLPSHCPQ
                     LEQLGAPTVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YIYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMLTFQQSYQQRVGEGRL"
     gene            19067..19771
                     /gene="yggS"
                     /locus_tag="ACHKTW_000291"
     CDS             19067..19771
                     /gene="yggS"
                     /locus_tag="ACHKTW_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HFO6461384.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNDQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPSLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            19789..20355
                     /gene="yggT"
                     /locus_tag="ACHKTW_000292"
     CDS             19789..20355
                     /gene="yggT"
                     /locus_tag="ACHKTW_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HFO6461385.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVT
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFKVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYVINMGVAEVLQATGNMLLPGLWMAL"
     gene            20352..20642
                     /gene="yggU"
                     /locus_tag="ACHKTW_000293"
     CDS             20352..20642
                     /gene="yggU"
                     /locus_tag="ACHKTW_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 family protein YggU"
                     /protein_id="HFO6461386.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            20650..21243
                     /gene="rdgB"
                     /locus_tag="ACHKTW_000294"
     CDS             20650..21243
                     /gene="rdgB"
                     /locus_tag="ACHKTW_000294"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HFO6461387.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDVLGGAPGIYSARYSGEDATDQKNLQKL
                     LETLKDVPDNQRQARFHCVLVYLRHAEDPTPLVCHGSWPGMITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            21236..22372
                     /gene="hemW"
                     /locus_tag="ACHKTW_000295"
     CDS             21236..22372
                     /gene="hemW"
                     /locus_tag="ACHKTW_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFO6461388.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     NDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRVEFIAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
     gene            complement(22527..23534)
                     /gene="yggM"
                     /locus_tag="ACHKTW_000296"
     CDS             complement(22527..23534)
                     /gene="yggM"
                     /locus_tag="ACHKTW_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HFO6461389.1"
                     /translation="MKKQWIVGTALLMLMTGNAWADGEPPTENILKDQFKKQYHGILK
                     LDAITLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWSVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFNYRFSQLESALN
                     TQKNSIPALEKEVKALDKQMVAAQKAADAYWGKDANGKQMTREEAFKKIHQQRDEFNK
                     QNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVPIQQKRDFDINEQRRQTFLQSQKLS
                     RKLQDDWVTLEKGQYPLTMKVSEINSKKVAILMKIDDINQANERWKKDTEQLRRNGVI
                     K"
     gene            complement(23651..24697)
                     /gene="ansB"
                     /locus_tag="ACHKTW_000297"
     CDS             complement(23651..24697)
                     /gene="ansB"
                     /locus_tag="ACHKTW_000297"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006528 - asparagine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HFO6461390.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINTDCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            complement(24873..25592)
                     /gene="yggN"
                     /locus_tag="ACHKTW_000298"
     CDS             complement(24873..25592)
                     /gene="yggN"
                     /locus_tag="ACHKTW_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HFO6461391.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGESSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            complement(25613..25753)
                     /locus_tag="ACHKTW_000299"
     CDS             complement(25613..25753)
                     /locus_tag="ACHKTW_000299"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6461392.1"
                     /translation="MQNCLMRYAYHAWKDARSSRPDKAFTPHPAWKTRTLLSIWGQQM
                     LA"
     gene            complement(25776..26102)
                     /gene="yggL"
                     /locus_tag="ACHKTW_000300"
     CDS             complement(25776..26102)
                     /gene="yggL"
                     /locus_tag="ACHKTW_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HFO6461393.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKTVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEERKLDEVRTSEL
                     FDVWWD"
     gene            complement(26102..26821)
                     /gene="trmB"
                     /locus_tag="ACHKTW_000301"
     CDS             complement(26102..26821)
                     /gene="trmB"
                     /locus_tag="ACHKTW_000301"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFO6461394.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            26982..28034
                     /gene="mutY"
                     /locus_tag="ACHKTW_000302"
     CDS             26982..28034
                     /gene="mutY"
                     /locus_tag="ACHKTW_000302"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFO6461395.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMARFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAAANNSWSL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRHWLAQ
                     RQIAADNLTQLTAFRHSFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            28062..28337
                     /gene="yggX"
                     /locus_tag="ACHKTW_000303"
     CDS             28062..28337
                     /gene="yggX"
                     /locus_tag="ACHKTW_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HFO6461396.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            28402..29481
                     /gene="mltC"
                     /locus_tag="ACHKTW_000304"
     CDS             28402..29481
                     /gene="mltC"
                     /locus_tag="ACHKTW_000304"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HFO6461397.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLQSRNNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQHTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            29683..30939
                     /gene="nupG"
                     /locus_tag="ACHKTW_000305"
     CDS             29683..30939
                     /gene="nupG"
                     /locus_tag="ACHKTW_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HFO6461398.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(30989..33124)
                     /gene="speC"
                     /locus_tag="ACHKTW_000306"
     CDS             complement(30989..33124)
                     /gene="speC"
                     /locus_tag="ACHKTW_000306"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HFO6461399.1"
                     /translation="MKSMNIAASSELVSRLSSHRRVVALGDTDFTDVAAVVITAADSR
                     SGILALLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGATPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKAELPRPFR
                     LAIIQLGTYDGTVYNARQVIDTVGHLCDYILFDSAWVGYEQFIPMMADSSPLLLELNE
                     NDPGIFVTQSVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGINAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSPLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SAYIRGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGVNL
                     LPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            complement(33286..33369)
                     /gene="yqgH"
                     /locus_tag="ACHKTW_000307"
     CDS             complement(33286..33369)
                     /gene="yqgH"
                     /locus_tag="ACHKTW_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgH"
                     /protein_id="HFO6461400.1"
                     /translation="MHAISLHSLAYRHFARNVSLSMLLLMR"
     gene            33522..34220
                     /gene="yqgA"
                     /locus_tag="ACHKTW_000308"
     CDS             33522..34220
                     /gene="yqgA"
                     /locus_tag="ACHKTW_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HFO6461401.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVAKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLTTPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWFA"
     gene            34326..34401
                     /locus_tag="ACHKTW_000309"
     tRNA            34326..34401
                     /locus_tag="ACHKTW_000309"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:34359..34361,aa:Phe,seq:gaa)
     gene            complement(34607..35704)
                     /locus_tag="ACHKTW_000310"
     CDS             complement(34607..35704)
                     /locus_tag="ACHKTW_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001682069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO6461402.1"
                     /translation="MVSDEKMSWEELLEEYFLIKNLKPDTEHSYRKVVRLFNEFMGGN
                     SWPGDVTARDIQRWRRHMLKVQGRSTNTWNNRVSHLRPILALGIRKKWLPQTENPLDD
                     ASVPGESKKKKTLTEKQMIKVYLTVQNFAEAEVKRQVVCDRMRNAMYPAWYWLTVLDV
                     LRFTGMRFNQLKHIRLKDVHEDEAVIALQLEGSKTRREWSVPIVTPLQISLKHLLACA
                     RANGAMDDDYLFDVCLYSEHYGPGKYKYCARRAHQSVRSFFRRLSRECGFNVSPHRFR
                     HTLATKLMEAPERNLHMVKSILGHRSVATTMEYVDLSLKVAAQTLEAELSLSTDEGTK
                     RRIHEARKHSYGYSEVEQSSDVSEVESTDTE"
     gene            complement(35743..37248)
                     /locus_tag="ACHKTW_000311"
     CDS             complement(35743..37248)
                     /locus_tag="ACHKTW_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001682068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraI domain-containing protein"
                     /protein_id="HFO6461403.1"
                     /translation="MFKLRKFFTSGSAVEIPDDGRSVPGTPEGFYAPLTTDSLLMPKN
                     RAQCLKQIRDNNMMPKPLCEAMCCAPVYSLLLSTQNVPAAREGRWSHAGGFGDLTLLH
                     TSYAVRLARAHMFPPGASAEEQAAQGAAWHAVIFWSALFYHLPLLAHLEGELFSGRGW
                     QPGISVPDEAYRFRFRQLPPQGTEAQQLAAVIAGGLLPEGAAAWLATVPGALQNLAGA
                     FWHQHPEMALIRDVLQESARQAESPLLNGGIVATASHIASAEVVTGEVVVSGKTESTV
                     APAVSGEEAAAEGNTVSGNAAESVQEQPEMRQQQAEKPTTPDSSEGSEDDAACEMESI
                     PTDEDTAMLVSMFAEEARAPEKGEDDSPSGEVVAPVSSTGEISAAGDDEEDFIAWLKM
                     GLSSGMIEANTPSARVHYIAGYVFLSVPDIFFLYRKLTGSQRERKNIQALFERACIHK
                     IREGERFFSVRLYSVPEKTGNYKTVSGYLVKGRYLYGYNKVPEDSAFIIFP"
     gene            complement(37241..38749)
                     /locus_tag="ACHKTW_000312"
     CDS             complement(37241..38749)
                     /locus_tag="ACHKTW_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HFO6461404.1"
                     /translation="MPHKPTEEQTAIIHWQGRKLVVNAFAGTGKTSTLVSYAMACPES
                     RMLYLAYNRAIRDEAERRFPFNVECKTSHQLAFARFGRHYQKRLVPGLFVTDVARKLN
                     TRYWALARVAMTGLNQFIGSADATPGRQHLPDKDEMKGVAPDEALRAVRTLWQAMSDP
                     DGTFPVTHDTYLKLFQLSGPDLSRRWDTILFDEAQDANPVTSALVLGQRCRIVLVGDR
                     YQQIYRFRGADDALSHPSLAGADRLWMTQSFRFGPAVAAMANLLLKRAGESREVSGCG
                     GDDEVLTMQEMRERIKHGKFGGHRAVLNRTVAGVIGTALMAAMAGRKVYWVGGIEGYR
                     TEALEDLYWFQMDMPARMHSDRLRRDYRNFDEYRHIAKVTKDVEMNQSIRLLELCFPL
                     PKKLELLREHTVTNEREADITVSTAHRSKGLEWERVVLGDDFQDIADPLMSEQERRDE
                     TNLLYVAVTRARRTLVLNELMQQLAEEKSRTKEKTTSPHERGTEKAVEGKDV"
     gene            39287..39517
                     /locus_tag="ACHKTW_000313"
     CDS             39287..39517
                     /locus_tag="ACHKTW_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000135398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1187 family protein"
                     /protein_id="HFO6461405.1"
                     /translation="MASAQATPFPYKYCIQATIEKPGGTPVSWIRFQNTPMTKQECEK
                     LFSTEKEAGKSFTVKVGVTQFSCKENPPAPGE"
     gene            40017..40688
                     /locus_tag="ACHKTW_000314"
     CDS             40017..40688
                     /locus_tag="ACHKTW_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001023520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin co-regulated pilus biosynthesis Q family
                     protein"
                     /protein_id="HFO6461406.1"
                     /translation="MNILRYLSISIFAFLFSYTATASTQTSSSNNHAEKRAIGTLKFI
                     RADGHRPFGQAMQQLLPTGWRTEIARDVRLPARISWSGNDQWNHVIEKTLPRYSLKAT
                     FDTSGKRVLIQQAEKTVKSGLFSAPVTGVKKTETRHIEQGMTLRTGLEKWAAAEPCAT
                     GKWKVAWKTPVNYSIDARLVFSGNFMDALNSTFALYQQAEKPLYASVNKNQCVLIITD
                     IPGDS"
     gene            40689..41123
                     /gene="pilM"
                     /locus_tag="ACHKTW_000315"
     CDS             40689..41123
                     /gene="pilM"
                     /locus_tag="ACHKTW_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000539796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis protein PilM"
                     /protein_id="HFO6461407.1"
                     /translation="MGWLMPLIIALFIIGNWFHAGNTERAIKNTDEQQRQQQVYEILR
                     LTNAINDWKYHHPGDIREGKMLTVNETGFAPVYGTKHIISGQRTFVWLPFQPGLISAL
                     NKVSRNSALIAQVRDRKLLDNQGNNIGVPPSVIPENAIVLIN"
     gene            41373..42974
                     /locus_tag="ACHKTW_000316"
     CDS             41373..42974
                     /locus_tag="ACHKTW_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilN family type IVB pilus formation outer
                     membrane protein"
                     /protein_id="HFO6461408.1"
                     /translation="MKKFFCAVTALSVVLSGCSSFERIRATEKNASDEITRAERIAGE
                     LRKQRAVVRETNQQWINTTPLPEKTVTRAIPDCPVVINTREAITLHQIAQRISESCHI
                     PVQIMSDVWTLLGTGENRTQRISGDIPPPDTSGMQPLSTVGLSSAAPSTASLPELNVQ
                     GLPALLNTVSSRFGISWRYVKGKIEFHWLDTRTFPVTYMDSQVAYNARVVSGTMSSTG
                     SSGGSSGSSLSGDASNTQTTAVDMKSTLYEDTKNAVNSMLTPGIGRMVLSTGFLTVTD
                     TPQVLDTVKNFMEERNREMRRQVVLNIEILSIKKTSKEQAGIDWNAVFSDGHLGIDLT
                     SAFTNAADEVVTSGVSIIDGKLTGSKAFLKALSSQGKVSVVTQNSAVTKNLTPVPMQV
                     ASQQGYIESVTTDTTANVGSSTSLNAATITTGFNMTLLPFIQPGSHRLQLLFSMSLSD
                     KPVFEVFESGGSRAQTPSVELKTINQTIDLKSDQTVILSGFQQFSRKSLRQGVGTPSF
                     FGLGGGVDSADDDTILVVLITPHVI"
     gene            42998..44293
                     /gene="pilO2"
                     /locus_tag="ACHKTW_000317"
     CDS             42998..44293
                     /gene="pilO2"
                     /locus_tag="ACHKTW_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001169834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4b pilus protein PilO2"
                     /protein_id="HFO6461409.1"
                     /translation="MQKKIRPVSLISGKQAFLAGLDWKNIPPGYRNARRFARSQGAEL
                     YLSCHNHDGENGDEMMIALLSRQDADIPRNIRQCLSLAMLIRPLLKSGGYAIFIIGND
                     ENDDAELTCAFVSVINGILVNDVTGTPTEITEARNTFLMLNAEPENGWVRYEPDGFSP
                     DSRCIPLPLSNLISTRKHPAEARLLPVSRGPQLMTALLIITLLGASWYGWQRYEIWQA
                     EKAEREESARKAAEARITPPWTGQPETGEFIRQCSTTWNQTPLSAAGWRYTLAECSTD
                     GNSGNLRASYTNTAGTTVTAFIRRITELTDTRPFIVMPEGNSGGVALPVTFRLPDNPV
                     PVDSLPETSDLQERLTTLAQSVQLQIDWQEVNNSFTDENGNIIQPPWKEYDLQIQTLL
                     PANQLAERFSEPSVRFLSVTRQLENGRFRYQFTGKYYAR"
     gene            44283..44744
                     /gene="pilP"
                     /locus_tag="ACHKTW_000318"
     CDS             44283..44744
                     /gene="pilP"
                     /locus_tag="ACHKTW_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000543769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis protein PilP"
                     /protein_id="HFO6461410.1"
                     /translation="MHDKTKALLIAALLLPAMAVAGNENDASQNKQLQIEQIQEETAL
                     YEAMLAREKIRHELRTLEQGISPVSGSVTGHQPENAAGTSSGSQALSSPRLKEIFGIH
                     PQLSARVILPGGTETDVRAGQALPGTLFMVEQITPDSVILEYNGNRQTLRP"
     gene            44754..46274
                     /locus_tag="ACHKTW_000319"
     CDS             44754..46274
                     /locus_tag="ACHKTW_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001115301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HFO6461411.1"
                     /translation="MPETIVLPPEDIRDFVAIQHDADGKIRVKVVEAKKHITSVQRYL
                     TLLRSRYPGKISKFDFTTLDVIAALNSNNAERAPGVLSDGGINTVQRKVLDYIRKAAQ
                     LDASDLHITPGRDNTDFTYVEARVHGELEVLDILRKEEGLELLGATYSGMTDVIKGTQ
                     FDPGVPQDARLSEQYLKLAGLFGARYSHYPCVGGLYAVLRLIKDDSQQIPTFSMLGYH
                     PDQERTLRRILQRPEGIITLSGPTGSGKSTTLRTASAAYLEQYGFNNTGGILLPRRRL
                     FTIESPPEGRIPGAIQTAVMDTAQGWVDSIKSALRLDPDGILNGEIRDHDSAITAIKA
                     AMTGHLMLTTIHANDPINILERLEMEGVQTRMIADPQLFIGLLSQRLVQLICPHCRRP
                     WHEVATGRTDDERRLVEGFCNPDQVYLRNHEGCSHCWRGVTGRTVIAEVISPDARFFQ
                     LYREKGRIEAKTYWHRELGGMTRNQHLLGKINSGQVDPLAAHYISPIDEDSYTLLH"
     gene            46276..47376
                     /locus_tag="ACHKTW_000320"
     CDS             46276..47376
                     /locus_tag="ACHKTW_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000670135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HFO6461412.1"
                     /translation="MRFLEKLNNTDIELTALTVWIARKTFALNRVEFYESLGGMIRDG
                     TPSLRALEFLCNVETDFGTRKGTSGMYFLAKECIASIKSSGMLSGALPTWVPPEEATL
                     IRNGEERGNIADALFQVARTVKARREMTSSLIAVCLYPLLLLSLCVVNMYNAHTRIIP
                     IVSAFATEDKWSFQMKVLAGMSEFIIGYGLWLCGAIIALTVLIRFSFSRYTGPGRRML
                     DRVPPWSLYQTLHGVNYLFSISSMLQINIPLSQALLRMEKGAEKNKWLKERIQAIRKH
                     VLSGQNLATAMRNSGYDFPSRVCINKLLLNSEREDSVAAMAVYADQWLEEAKKRVKRS
                     GLIITGISGGFVFFFILNMISAIYSISDMLKQ"
     gene            47427..47963
                     /locus_tag="ACHKTW_000321"
     CDS             47427..47963
                     /locus_tag="ACHKTW_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009447271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4 pilus major pilin"
                     /protein_id="HFO6461413.1"
                     /translation="MNFRARLFYYFSLKKEKEEDKGLTLLEIAGALIIIGIVVGLAMS
                     QMSSTMSSSDNQRELKNLQTIATKMKQYKFQGRYTGSDYIKTLTESGSLPADMIAGGN
                     KAKNAWGGDVTIKATTDKYGYTITSNNVPKENCVELINSLRSSSMFTKIMNTTPATVD
                     PVTVCSKDKNNITLETNS"
     gene            48022..48498
                     /locus_tag="ACHKTW_000322"
     CDS             48022..48498
                     /locus_tag="ACHKTW_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HFO6461414.1"
                     /translation="MKWLYFSVLILFSHTSIASVMNNNHLDSCFIQAGKRYLIAPELL
                     KTIAQQESGLVATAINHNKNKSGKIISTDYGIMQINSSHIPELKKLGVIKDKDELLKN
                     PCLNIHTGAWILAHHFQRCGVNWECLGSYNAGFSKNNTARRMIYARIIHNRYFRKD"
     gene            48515..49165
                     /locus_tag="ACHKTW_000323"
     CDS             48515..49165
                     /locus_tag="ACHKTW_000323"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001292581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HFO6461415.1"
                     /translation="MSELLPICIILIVLAAILYLAYSAATVYLSQVHNTRPHNAAVCI
                     CCLLAVLSICLNGAYGVDATGLLFLSILTGLLTVMTLTDIAVCRLPRIFTLSLIILGA
                     AFRYSQDSLAHALLNASLWFGMIYLLRRIFLAVKGTEALGLGDVFLIAGIAMWTQAQH
                     TPLIITAAASGAFLFILIFCRRRRQQALPFAPFLCASLYVLTLFPDSVIHTSEIFT"
     gene            49162..50379
                     /gene="pilV"
                     /locus_tag="ACHKTW_000324"
     CDS             49162..50379
                     /gene="pilV"
                     /locus_tag="ACHKTW_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shufflon system plasmid conjugative transfer
                     pilus tip adhesin PilV"
                     /protein_id="HFO6461416.1"
                     /translation="MKKFRLDDDKGFILLEILAGLILIGIATPMIYSEIEDWLNEQLY
                     QSAALHADEYNNAIKNYVADKTSSLSSKTITVNELIQQGYLNNGFSRSPFGHSYITGI
                     RKNNLSGRLEALTCTTGGQDIKEDGLRRIAGQINGLGGFMLQNNSVTGAFGGWTDLGS
                     NYQITCNKGHIAMRMAGKDLEESDRLYRYSVPGRPDLNRMHTGIDMNNNSITNINEAS
                     GKNARFSGDVTSNRWLHPNGGGFTMTDSQWIRAVNNKGITTEGEIKGGTVRGQTVTGG
                     TVRSDGRLTAGEYLQLNGIANAGTHCSPDGLVGRDNSGAILQCQSGIWTTTKMNFTTS
                     TYNIGKNTRNLSIGVHAYCSWTYLNGSPFGGFQQIYADKNKVWYVNNYAWGNYESGGT
                     ITVTCLNLPGAGI"
     gene            50723..50962
                     /locus_tag="ACHKTW_000325"
     CDS             50723..50962
                     /locus_tag="ACHKTW_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000271304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6461417.1"
                     /translation="MYASGGNGAGGGDCANTSNLQVYVNGTFIGMNASNNPSYGKTAF
                     ISFAVPVGASYQIISRPTQNYACGNGVFSVYAYQM"
     gene            complement(50967..51356)
                     /locus_tag="ACHKTW_000326"
                     /pseudo
     CDS             complement(50967..51356)
                     /locus_tag="ACHKTW_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001168069.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
     gene            51420..52544
                     /locus_tag="ACHKTW_000327"
     CDS             51420..52544
                     /locus_tag="ACHKTW_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000486721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFO6461418.1"
                     /translation="MFRKIKIRKMTLNRALDKYLKTVSIHKKGHLQEFYRVNVIKRHP
                     MAERYMDEITTVDIATYRDQRLAQINPRTGRQITGNTVRLELALLSSLFNIASVEWGT
                     CRMNPVELVRKPKISSGRDRRLTSGEERRLSRYFRDKNQQLYVIFHLALETAMRQGEI
                     LTLRWEHLDLQHGVAHLPETKNGLPRDVPLSRKARNYLQILPQQINGNVFSYTSSGFK
                     SAWRTALLDLKIENLHFHDLRHEAISRFFELGTLNVMEVAAISGHRSLNMLKRYTHLR
                     AYQLVSKLDAKRKQTCKIAPYFVPYPATVGNRNGLFIVTLHDFDLETRAETRELAISH
                     ASVLLLRTLAQAAQRGERVPTPGELPANIDARVMICPLTS"
     gene            53105..53926
                     /locus_tag="ACHKTW_000328"
     CDS             53105..53926
                     /locus_tag="ACHKTW_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HFO6461419.1"
                     /translation="MKPNSTGIAARMILSLDRARICEALLNNRQLQPTPLQVRYPQGV
                     REALSIMSEQLSLSVSDLTRILVEEALSEMFLPADNSVRRLHDRMEYLMQVHELSPVT
                     MATLLAPWNIRPAVFREPDRLTDYLTGEILASLADWFYLSPEWLNGRVHYPLCHPGDW
                     PETQDAFCRLISEDDNIDIILWHGFPFSGEHAQEYCGVLLRQKKKINNAIIYPVLSLY
                     PTGMNKKKEGWFQMARKMSPDIPVRAVTLSPAQAEYLVTGKILPAELFRIPLSPW"
     gene            54016..55218
                     /locus_tag="ACHKTW_000329"
     CDS             54016..55218
                     /locus_tag="ACHKTW_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001532112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
                     /protein_id="HFO6461420.1"
                     /translation="MMMKKHITRTLVASAVLFSFNSAAATSYSEARNDAMGGTGVASS
                     HYGVAPLANPALLTKAGPDDDFSLLLPSVGAQLSDPDNITDNADRISDDWKAFDRAID
                     SNHGVPEAAARLKERLRDFRHTHAAAQLGVSAVAALPGDRLSAALMVKSHGTVSVDGK
                     VSDADLTYLEEVANSTGQEVDKSRLTSQAFARAALITDVGIALATELETAGQKWSLGF
                     TPKFQRVDLFNYNVLVRNYDSSAFKGDRYHNTKNGINADIGASTDLDDNWTLGLVAQN
                     LIPRSIETKEVNGITETFRIRPQVTAGVSWHNAMFTTAFDVDLTPASGFTSDSNRQFA
                     AIGAEFNAWKWAQLRAGYRQNLAGNDGSAFTAGVGISPFDVVHLDVAGLIGTDNTYGA
                     VAQFQFTF"
     gene            55578..59435
                     /gene="sigA"
                     /locus_tag="ACHKTW_000330"
     CDS             55578..59435
                     /gene="sigA"
                     /locus_tag="ACHKTW_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin SigA"
                     /protein_id="HFO6461421.1"
                     /translation="MNKIYSLKYSHITGGLVAVSELTRKVSVGTSRKKVILGIILSSI
                     YGSYGETAFAAMLDINNIWTRDYLDLAQNRGEFRPGATNVQLMMKDGKIFHFPELPVP
                     DFSAVSNKGATTSIGGAYSVTATHNGTQHHAITTQSWDQTAYKASNRVSSGDFSVHRL
                     NKFVVETTGVTESADFSLSPEDAMKRYGVNYNGKEQIIGFRAGAGTTSTILNGKQYLF
                     GQNYNPDLLSASLFNLDWKNKSYIYTNRTPFKNSPIFGDSGSGSYLYDKEQQKWVFHG
                     VTSTVGFISSTNIAWTNYALFNNDRVNNLKNNFTNTMQLDGKKQELSSIIKDKDLSVS
                     GGGELTLKQDTDLGIGGLIFDKNQTYKVSGKDKSYKGAGIDIDNNTTVEWNVKGVAGD
                     NLHKIGSGTLDVKTAQGNNLKTGNGTVILSAEKAFNKIYMAGGKGTVKINAKDALSES
                     GNGEIYFTRNGGTLDLNGYDQSFQKIAATDAGTTVTNSNVKQSTLSLTNTDAYMYHGN
                     VSGNISINHIINTTQKHNNNTNLIFDGSVDIKNDISVRNAQLTLQGHATEHAIFKEGS
                     NNCLIPLLCQKDYSAAIRDQESTVNKRYNTEYKSNNQVASFSQPDWESRKFNFRKLNL
                     ENATLSIGRDANVKGHIEAKNSQIVLGNKTAYIDMFSGRNITGEGFGFRQQVHSGDSA
                     GESSFNGSLSAQNSKITVGDKSTVTMTGALSLINTDLIINKGATVTAQGKMYVDKAIE
                     LAGTLTLTGTPTENNKYSPAIYMSDGYNMTEDGATLKAQNYAWVNGNIKSDKKASILF
                     GVDQDKEDNLDKTTHIPLATGLLGGFDTSYTGGIDAPAASASMYNTLWRVNGQSALQS
                     LKTRDSLLLFSNIENSGFHTVTVNTLDATNTAVIMRADLSQSVNQSDKLIVKNQLTGS
                     NNSLSVDIQKVGNNNSGLNVDLITAPKGSNKEIFKASTQAIGFSNMSPVISTKEDQEH
                     TTWTLTGYKVAENTASSGAAKSYMSGNYKAFLTEVNNLNKRMGDLRDTNGEAGAWARI
                     MSGAGSASGGYSDNYTHVQIGVDKKHELDGLDLFTGLTMTYTDSHASSNAFSGKTKSV
                     GAGLYASAIFDSGAYIDLIGKYVHHDNEYSATFAGLGTKDYSSHSLYAGAEAGYRYHV
                     TEDAWIEPQAELVYGAVSGKRFDWQDRGMSVTMKDKDFNPVIGRAGVDVGKSFSGKDW
                     KVTARAGVGYQFDLLNNGETVLRDASGEKRIKGEKDGRMLMNVGLNAEIRDNLRFGLE
                     FEKSAFGKYNVDNAVNASFRYSF"
     gene            59735..59968
                     /locus_tag="ACHKTW_000331"
                     /pseudo
     CDS             59735..59968
                     /locus_tag="ACHKTW_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(60164..60409)
                     /locus_tag="ACHKTW_000332"
                     /pseudo
     CDS             complement(60164..60409)
                     /locus_tag="ACHKTW_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696717.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
     gene            60806..61198
                     /locus_tag="ACHKTW_000333"
     CDS             60806..61198
                     /locus_tag="ACHKTW_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000423941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6461422.1"
                     /translation="MELTADLLKSLNNIRQNATIYVHAYHYTDDEKCVMAPRSSLPSL
                     ELTVLQSEKIIDLLLSGYGYIRHDGYVQGYGEKTVATFTIQEGKHHHFRTNLALNKTG
                     NIADNALNHWLHRIRKTDIIVLSVVLTE"
     gene            61258..61539
                     /locus_tag="ACHKTW_000334"
     CDS             61258..61539
                     /locus_tag="ACHKTW_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001672444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6461423.1"
                     /translation="MKTNKKDTKWYIFYRENSGEDILLEMSSFKECLSASKELMTPSN
                     YMICIERNGERIKRWDREIIAGSNKWINCPPDNFEILGELITINRIIKK"
     gene            complement(61529..63322)
                     /locus_tag="ACHKTW_000335"
     CDS             complement(61529..63322)
                     /locus_tag="ACHKTW_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6461424.1"
                     /translation="MEKVSIRKINRTEHFPSLNLPSDTSWVVWWYGFLKKNKRRGEQP
                     KVAITFREVQTDGTLCDRYYEQECLLTDLSFFKIGSVWTRGRCVAEILMEEKIFNVDF
                     SYANGGWKINSLYQAYKEGVSLPYDFSDYQLKSPYDKNWLLEFLTDDADKVIIHPLEF
                     FYRCYGHSDELKRVILTYSGNELRNRIFSEAYVEGDKIFYIKLRKRMNKYDAPFASFF
                     ALDGFTRKVIDRIQRQLDVCEDRKIYLKVAPWFKGKARIKVKGFWLENNVTFLGLSVT
                     GVSLPAGYTINYDRDNSNLVDEKAEQGSRQAWSNVPVRHIKNPDEPFSVDNAFSPDSG
                     ASTLEVEASVFEFLDDGCELSPLRRKKAKYISVTKNESDLHSLYSVNEVVDEQNSIST
                     GYMSLADKNEPERLYMESKGIVRDMWNAMVYLRGAYSERIKSLDWYTPESSFLNTQEP
                     ELVAIPEFNESEVENDKISKSILNWPYLDVVNKKDIRGFLISRMLIDDNMIYFMEIQR
                     RVCNEETMNSFSEVEKFKGLVFTLDNECELDKWVSLLAHESRFVKGILQKIVGKCPGT
                     AMTYKHSPAKNEPVACYSALLNALSKVGVTF"
     gene            complement(63477..64175)
                     /locus_tag="ACHKTW_000336"
     CDS             complement(63477..64175)
                     /locus_tag="ACHKTW_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001121871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6461425.1"
                     /translation="MKVVFNVMDGFDKTFLPLEFSGFHGRNGYCYLRVQIKHGFIVFS
                     CAQLLNYYRTSVTNAIEQVREAAVNALFREGVLSYTQQKEFLDILKTSQRVDKEIDSQ
                     LWDYINANSIWFEYYNHNESLFLNDHFHIASFEGNRNPLWKKTSLEDLERAYPEFDFV
                     IHKHHLEKWINGGLSPENVKKTIKEKGWSNKMLAARWGCTEVWVSKIINDENRKVQWN
                     DAINGLPVISDNMI"
     gene            complement(64340..65026)
                     /locus_tag="ACHKTW_000337"
     CDS             complement(64340..65026)
                     /locus_tag="ACHKTW_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HFO6461426.1"
                     /translation="MTTFIDHQKAFILLFNQTARYHKRHKVFEDFVSCSVIALENRLQ
                     FSEAREQKYLRIVSGYEKQDVINMAHLLAHVIEGLEQGFCDFLGSVFMQLELGDKYRS
                     QFFTPWGVACMMAQMQLGNVKALFENKPFITLSEPACGAGSMILAMADTLNRSGYPAY
                     RRMWVSATDIDPLAAGMAYIQLSLCGVAGEVVIGNSLCNERRRILLTPGHYLGNWSYR
                     LRHVQEQAAA"
     gene            complement(65149..66096)
                     /locus_tag="ACHKTW_000338"
     CDS             complement(65149..66096)
                     /locus_tag="ACHKTW_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HFO6461427.1"
                     /translation="MAEWCRNRFEITGKSVCLDVLTQWIEGCETPRYRHAIQQSIQLF
                     LIGCAGLVKPTRTTQYPPYPALVRHGTGMSSPANQAFEQWLGLLVKDAVLDEETIKTI
                     DRLYHQSCIGAVRWGNLPDNAREIITTLMHCQYSDWFGLVGLSEHIDAEACWSRLSDY
                     PEQAQPCDMLMVIPPRLATELNGSGGLLQGISTTTSLYSRIYGVEWPSGHNVRWVRDE
                     MSSLVLLTDTPWYPPSGELVGEISKVFDCEIRHWYSEPVRGIQGYNCYDGGEHTDSDP
                     QAEWPGREKLPQPRLYLVEERAEEQTEAAPLPVPLASGQ"
     gene            complement(66204..66638)
                     /locus_tag="ACHKTW_000339"
     CDS             complement(66204..66638)
                     /locus_tag="ACHKTW_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001101430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPD29 domain-containing protein"
                     /protein_id="HFO6461428.1"
                     /translation="MNVKSVTEVDDAVVARVSDVLEFAFPGQKFNVLKVCDSGVYNMI
                     NVSWLDGPTEAEVRFITRAFEGKNGLRFVHESRKFSNEFVQECIDRLRKKYGQSNVPP
                     DVTVARYWKNDLWKIKTDRFPGNIDVAINEMGTETSKYRKVV"
     gene            complement(66715..67053)
                     /locus_tag="ACHKTW_000340"
     CDS             complement(66715..67053)
                     /locus_tag="ACHKTW_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6461429.1"
                     /translation="MRPDLNILPGDSGCSVWFYNGMSQPRLLAGSIAGLLTDVTITSN
                     YRGDVTSEIHDVVQEWLATGRGNLADLKEELWYYNLYINPSADELMNASRRYGLGHTT
                     WLKDFISNAV"
     gene            complement(67121..67480)
                     /locus_tag="ACHKTW_000341"
     CDS             complement(67121..67480)
                     /locus_tag="ACHKTW_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6461430.1"
                     /translation="MYSRFITGYWHLQSQSALLAHLCQLEGELAILRAWQRLGITPVP
                     EDEDEPSPLYSILWDVGEALGWLLYDLEMENAPAPGTIFHEVLDRVMSLGHETEHSIW
                     ADSISTGKRYAAIPDAF"
     gene            complement(67591..68574)
                     /locus_tag="ACHKTW_000342"
     CDS             complement(67591..68574)
                     /locus_tag="ACHKTW_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArdC family protein"
                     /protein_id="HFO6461431.1"
                     /translation="MKTKKHTSCPVNKTPERDLYQLVTDRVIEAIEKGVAPWQRPWRS
                     VRTHSGDPMMPVNALTRSAYRGINVLLLWLAAEEAGYSSNCWLTYRQAQALGGQVRKG
                     EKSTMAVIYKSWHKQDEDENGNRIFDAEGNPVMVDVPVLKANPLFNVEQCDGLSLPEV
                     TTTVTENGANDTLNAVADRVLTMVNACGVKIHSLPQDRAYYTPVFDRIVLPLSSQFTS
                     AEDYWSTLLHELVHSTGHAKRLNREGIVSSGGRYGDECYAFEELIAEMGSAFLCAQLG
                     VTGNVQHENYIGAWLKILRADKKALFSACRQAREATEYLLSVHDTPAEKAA"
     gene            complement(68667..68804)
                     /locus_tag="ACHKTW_000343"
     CDS             complement(68667..68804)
                     /locus_tag="ACHKTW_000343"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6461432.1"
                     /translation="MTFPTQTERFFFYLLYRVPASVAWVSDTLKTFDFERHSLVMLAL
                     W"
     gene            complement(68782..69261)
                     /locus_tag="ACHKTW_000344"
     CDS             complement(68782..69261)
                     /locus_tag="ACHKTW_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012601728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6461433.1"
                     /translation="MNSLLILLYVVVCRACTILRVIAPVSSVRAWCEYQLEGMTILRD
                     AYSSLAKADGMQNDAFEYLESIARHLGYESGGDQLKSGLFRPFLWHIRAKGHRLGAVM
                     EMAVWQAWKNGCNDVCNPWGDVYNPWDRGWNDGWTECLDQRIAGLLKAVCIDFSDAD"
     gene            complement(69362..69742)
                     /locus_tag="ACHKTW_000345"
     CDS             complement(69362..69742)
                     /locus_tag="ACHKTW_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6461434.1"
                     /translation="MGLDIFFLGRDRVTDAVVSVPETREVGYFRKVNPLIAWFETHCG
                     PVENAVERPVSRTELEALLSDLECLTPENCREFFPTTEGFFFGSQEYDQYYWKDVEIV
                     KSWVRSTLDSFDFERKILLLWAWW"
     gene            complement(69907..70308)
                     /locus_tag="ACHKTW_000346"
     CDS             complement(69907..70308)
                     /locus_tag="ACHKTW_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001672448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6461435.1"
                     /translation="MFIPLLLRADMRITPFFHLLRQCLFNQKRCVHPQQPWSAPQLRH
                     LYCTPWFINATGNLSVRACIVSTNGALRHCFEFREGSHSREYRYWFEALPLSVVRFNV
                     FTGRISARRTLSAASRMAAQLTSFRYRFGLR"
     gene            70840..71571
                     /locus_tag="ACHKTW_000347"
     CDS             70840..71571
                     /locus_tag="ACHKTW_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger-containing protein"
                     /protein_id="HFO6461436.1"
                     /translation="MKSKYKKLKDELLRIAKACAPTPEDMLVYTGRARRLASFLKDAN
                     IQISSANSIKLRHIECYFQQRYRTGVSSKILREELDTIKHVLTHCGKRNIVKNERLTY
                     TSLNIADVRPIVICPYCGNKTNLIKGALMPFSISAATENKYYWICPPCNAWVGCHKNS
                     GRPLGTPAKENLRILRTKVRKLFDNYQQRANISRNEANIWLSRKLNCHIHECHIGYFD
                     EDMCNRASEIIITEINKNTYPPDSF"
     gene            complement(71575..72492)
                     /locus_tag="ACHKTW_000348"
     CDS             complement(71575..72492)
                     /locus_tag="ACHKTW_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase domain-containing protein"
                     /protein_id="HFO6461437.1"
                     /translation="MARKSSRSKVELFRKSFISLARECGGSYATTADRMRIAKYFLNY
                     LRENGIKLRQTSSIKTRHISGYLLSRKAAGISHRTIQNERAVMRAILEKDGRLKIADP
                     SNPLLSNEALGLKDTCRDGKKIAVSPDEFWKAFAKVEKKNPGVAAAMQLSYVLGLRTK
                     EAVQSCKSVQSWLRELNSGCDSLSVVFGTKGGRPRNTTIIDRDAVRHALLYAQKIMDE
                     HDGKLIDRPNIKQAINVFRYHARKAGLSGVKSPHSMRYHFSQEARKFYRKNGYGESEI
                     YARVSMDLGHGDGRGRYVKQVYFNGSDES"
     gene            73235..73501
                     /locus_tag="ACHKTW_000349"
     CDS             73235..73501
                     /locus_tag="ACHKTW_000349"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6461438.1"
                     /translation="MPSSSRKTVVTSALKGVQICPYIRDVSLQYSQLLSGYGGVKLTT
                     RRRSSQVIWSIGGRRSDMGHPKAFLTGHYGTLVVAEATVNADAG"
     gene            73683..74354
                     /locus_tag="ACHKTW_000350"
     CDS             73683..74354
                     /locus_tag="ACHKTW_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6461439.1"
                     /translation="MEVSVIFFWVITALIAILFLKSAITYKIKKRNKTDIVLYILAFL
                     SAGYTFQYIYNDNIFSALRSIGLFVTLIVLNIVFNLLFSLLCILSKMILMSQHIRKMQ
                     KKLKQMPVSDFIYYLMKKHNKYPTYVLHMPSNNIFEIGYNIMNTDLVIGEKIYFRTLS
                     NRSLYFTLTQAPLIATIKDDVFCTLHDYYNHNNETKSTIDNYISKIKDNHNTPWLLNN
                     ENVHE"
     gene            74347..>74623
                     /locus_tag="ACHKTW_000351"
     CDS             74347..>74623
                     /locus_tag="ACHKTW_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001303895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin immunity protein"
                     /protein_id="HFO6461440.1"
                     /translation="MNKKQSANSRDKTKEHKHMNRKFYFKNILWGMCPIIISILHLIY
                     DTPTEASIKVDLFLILSGLALPCSIYLTKTITLKYTSISFWKKPFFWS"
BASE COUNT        19584 a        19493 c        17368 g        18178 t
ORIGIN      
        1 gatccctgct gaggcacagg aggtaacgta gctatcctcc gccccggtaa agcgagagac
       61 cagctcgccg gttttgtcca ccagctggtc aatctccacg aaagaggatg ccgcgcgcgc
      121 ggtggcctgc atcacctccg gcgcgacgct ggagacgccg agaatcgtca tcttgccgca
      181 ggcgttaatc acctgtttca gtcccagttg ttgatagata ttctgcgtca tggcttacag
      241 cactcccagc agcgagcaga ccacgctcaa cgccacaatc gacagcagaa tcgtggtgta
      301 acgcggaccc tttttcacca aatagaagta gatggcgaac actgccgcca gcggcagcag
      361 gccgggcgcg atggagtcca gaatctgctg taccaccact tcagagcctt tcagtgcgct
      421 aatttttaaa ggtgaggtta tcttgacaaa gctcgcggat agcgcgccca tcatgataag
      481 ccccagtacg ttagcgccgt agataagctc cttgatgcgc cctccctgca gcagactgat
      541 aatcgaatcg cgccccagcg tatagccctt gtgcaccatg ccgtagctga ttgccagcgt
      601 aatcgccggg tagagaatca gcggcaaaat accgcccatc gcgctgccat tggcggcgaa
      661 cgggataaaa atagcaatca acagcggcat caccgccgcc cagatgatgg agtcgcccat
      721 cccggccagc ggccccatca ggccggtttt aatcccggtg atagaggcat cgttaatctc
      781 ctcgccgcgg gtcttttgct cttccatggc gatggagatc ccctggatca ccgcgccaaa
      841 cgtctgctct gagttgaaga agttcagatg gcgttttaac gcctccacct gctcctcctt
      901 ttgcggatag agctttttga taatcggcgt catcgaggcg cagaaaatca ggctctgtaa
      961 acgctcatag gagctggaaa cttccgcgcc cagccagtag ataaaccacg ctttggtgat
     1021 atccgctttg gtcagcgtcc cggactcccg cgcgcgctca accagttcat gctgcattac
     1081 gtcgtcgctc attatgccgc tccttcattt ttcgccaggc ctttaatcag gaaggccacg
     1141 caggtgccga aaatcgccat cgccatgatg tccaccttga ggtacagcac cgcgaagaat
     1201 ccgccgataa accacggcag caggctcttt ttaccaatca ccataatggt gatggcgaag
     1261 cccagcgccg ggagaatccc gcccatgatt tcaaacgagt gggtcagcca gtgcggcatc
     1321 agcttgagga aactttccac tacgttctgg ccgaagtagt tggcggcgaa caccactggg
     1381 aaacgtagta ccagccccag cagcgccggg tagagaaagg cgcagcgcat aatggctgac
     1441 atattggccg tttccgcgtg tttatcggcc atatgcaccc aggccgcatt tagcgtgcga
     1501 cgcaactggt cgaggaatac gccgataacg ccaaatggaa tcgccagtgc aatcgccagg
     1561 ttcgggtcca tattggcctt aacggcgata ggaatggcga tacaggccgc cagcgccggg
     1621 tctgacggca cattgccgcc tggcgttgag gtcacaccca gatagaccaa ctgcatcccc
     1681 gcgccgataa tcatcgccgc aggcatattg ccgagcagca ggccgacaaa gaccgccacc
     1741 accactggct gtaacagcat ggcggagaag gtatagccaa ggcgcaaccg ggcaaaccag
     1801 taatacagcc ccatcaggct tgcaaaaagt aaggtatcca taatttgtta tcccatgttc
     1861 agagggtcag aattttttca ggatatcgtc cagcgactgc ggtttatctt ccgggatggt
     1921 ctggaaaaca gccttaatgc cgcgactttt caaatcgtta agaatgccaa cgtctttttc
     1981 atccagcgtt atgttctgga acaccgcttt acggtttggc ccgccgccta aaccgccaac
     2041 ctgaatatca gccacgtcaa agccctgctg caccgcttcc tgaatcgccg ccagcgacgg
     2101 gaacagcacc agcacgttgc cgtcgcccag ctggttctct ttccacgcgg ccgcgaagct
     2161 ctggttgcta aagcagtcca ctttgatatt cggcggcgcg gccatcaggt agatattttt
     2221 cataaacgga tcggcgtcca gctcgtcgct gaccaccgca atacggttgg cctgagactg
     2281 ccctacccac ttcgtcacca cctgtccgtg gataagacgg ctgtcaatgc gacataacac
     2341 gatgtttgcc atacgatttt ccttactgtt gtaattgacg aatatgggag acgacgtcca
     2401 ggcaactgtt gcgcccagct tccaccagat cggccacgca ggcggtcagt gggccatgtt
     2461 cgcgcttgtc cagcgcctcc agcagcagcg aggcattaag cccggagatc accgccaccg
     2521 gataatcggc gctcagccgc gccgccacgt tagaggtggt gccgccgata aaatcggtca
     2581 cgataagcga accttccggc atggttttca ccacggcttc gacgcgctgg taaaactcgc
     2641 ccagcgtatc gacgggcatc agcgcgattt ccgtcacgcc ttcgatttcg cccatcacca
     2701 tgcgcagact gttgcagagc tgcagccccc agccgccgtg agttagcagc agaatttcag
     2761 gtaacggatg aataggtaca gttgtggtca aaatagcctc ctttgctcca gtgtttacat
     2821 ttcatcagga gcaaaggacg tgccagaatt tgtgtcacgc acagaggaaa tgagattgga
     2881 tggggcgtta agccccgtaa accaggggat cagctgtaga gcagttcgta gatataaaaa
     2941 tattctgcgt ccgataaacg gatagcgtaa gcctcttcaa tcggcaaaaa ggaggatttg
     3001 atgacgctaa aggcacgagc gtccagatca agccggtttt ctagcgccat ctgtaacggt
     3061 ttgcggttaa tcacgatacg ctcgaccatg cagcagcagt ggatcagaaa acgtagtgtc
     3121 acctggcggc tcggtttcag ggagagcgtg ttggttaagt agttcagaac atcctcgatc
     3181 tctttcagaa ttcgctgcgg gttgagcacc gaaatatggt tgatgatgct ctccatggtc
     3241 agcgcgctga taaagcgcat ggcgctgcgc tccatctcca gtcggcgctc gctgcttgag
     3301 agatccggcg tcagcaggct gagcaccagc tccggccctt gctcggaaaa cagttcctcc
     3361 agcgagataa acggtatatc cggcagtccc ggctggaagg tgccgacgat ccctgccagc
     3421 cgttcactgg cattaagcgc ctgctgaata cgctccaggc tgcgcacctc gttatagtcg
     3481 agaatcacca tacgcgtgtc ctgcgacatc agctcgccaa agctctcctc cagcaccttt
     3541 ttgatctttt ccgccgtccc cattccggtt atacatgaga taaccagcac tttgccaccg
     3601 ctctcttgct gcggcgtgca gagccgacaa gggatatttt tactctgcat aagcacagcc
     3661 aattgcgata gatcgccagt ttcgtagctt aaatccagcc caacctccag caggctggtc
     3721 agagtgatat ttggcaacaa cagaacgtca atctggaaca gcttgctgat ggtgctgcca
     3781 aaatggacca gcgatccgat atccaccatc agtatcagcc gctgccagca ccggtcctga
     3841 atcatccggg tcagggtttc cagggtgtca tgcaccgact gctcaaatgg catatcaatt
     3901 gcgtagaaca gatcgcgctc cagcacccgg ttcacatatt gcgcctggct cgttgcggtg
     3961 gtcgcaccgt gagcgattag aatcacgccg caatccgggc tggtgtcgat gcgctggcgg
     4021 tagtggcgac actctttcag gaacaggcac agccagacca cctccgtcgc cgggcactga
     4081 atatgcaaca actcattgat tttgcggcac agaagcgtgg cattgtcata ctcatcttta
     4141 cagcgatcca gaatcaggct ggatgaatag agttgaggta taagccctcg ccgcacatag
     4201 ccaatcagtg caagaaagtg tttacgtaac gggttcgcca ggttttctgg caatgtaaat
     4261 cctaatacct gctccacgta accgatcagc agcgtcacac gctcctcaat ttgatcgcca
     4321 tagcgcggtg gatgcgccgc actatggcgg ctataaagac catattcaaa aatcgaactg
     4381 agtttgtttt tcaggatagc cagcgtttct gcgggcggca cgttactgtt gcgcagattg
     4441 acatattcac gagtcaggaa actgtaaaac aggtcgctct cttctatttc cgcgctggtc
     4501 gccaacgaat tttttaacgc gggcaacgta cgcgcatcca cgttcagacg atctttaccg
     4561 ccaaacaacg catcgaccag cagacgctgc tctggcgtgg tattaaacga catttccgcc
     4621 agtcgtttat ctaactctag cgtatcatta cgctcggtca ttcccgacgc ccacgcctgc
     4681 gcgcacaaga actgaatatc acttttaagc tggccgatat tgccttccgg gggtttactt
     4741 aacaaccaat gcaagagcgt tttatcaagg cttatggtac gttcaatttt acgactttca
     4801 cgctgaaaga agcttacaat caattcgagc tgctcctcga ccgaacgctg gcgaataccc
     4861 ggtaagtcaa tacatacctg gatacgccgc tggaaggtac gcaatagtgc cgagttgacc
     4921 gactctgtag tggcgcaaat aaggcgcacc gaaattgaat atgttggccc acttgaacca
     4981 agtgggcggt attcaccttt atcgagaata gaaaagagct tctcttgtcc ttcgtacggg
     5041 aggcgatgca cttcatccag aagtaaataa cctccgtcag cttgctccac caatcccgtt
     5101 ttattctcac ttgccccggt aaatgccccc ttatggtgac caaacagatg tgaggaaagc
     5161 aactccggat tatgagcata ttcggcacag ttaaagtaaa ccaacggagg tggcgaacct
     5221 atggtgtgct tgcaagcgaa gcaatgcatt aactcagcaa agaatgtttt gccaacacca
     5281 gatgcaccgg ttaacagcac atgtagtccg tggggataaa gcaccgctgc gcggcctttt
     5341 tctaccgcat cacggagact acggtcgtat ccgataagtc cggaaaacgg atcatcatct
     5401 gtatattttt ctttctttgg caataaacta gccactgaat gaactatgca ttcgcaatca
     5461 tccagttttc gattaatgag caattctagt gcgtgacgat gtagaaaaaa aacaggacgt
     5521 ccctgacttt ttaccaccag gccttcattc cataattgat taagatcttt actaaccgag
     5581 ttgcgtgcca gcccaaggtt aaaaccaatc gcctcagcag taaatgcttt ctccttcgct
     5641 aaatcagaca gttccagccc atacgtcagc cgctccagct ccttgtgaat aacatcaatc
     5701 cgtctcattt ctttaccgct taaaaaacaa tagttgttca gaacatacac tatttgtaaa
     5761 cgtaagaatg agaactatcg ggaagaatta atgttccttg tggcaatctg gtttctgaat
     5821 attcgtgttt taaatattac ttatggtctg aaacaaccag gcaggtgaac agagctaaga
     5881 aaaagtagcg tagaaatata aaaacatata tcgcactgat gaatgtttcc cggcatgact
     5941 atgcacaccg ggaaatattt agtaaattac tcgccctttt tcgccgcctg aatatacagc
     6001 atctccagcg ccagcgtcgc cgcagccaga gcggtgattt ccgactgatc atatgccgga
     6061 gccacttcca ctacgtccat cccaacgatg ttgagatctt tcaggccgcg taccagttta
     6121 atagcgcgat cggaggtcag gccgccaatc actggcgtac cggtgcctgg tgcaaaagca
     6181 ggatccaggc agtcgatatc aaaagtcagg taaaccggca tatcacccac aatctgtttc
     6241 acttgggcga taacgtcatc cacgctgcga tcgttcacct ggcaggcgtc cagcacggta
     6301 aagccgttgt ctttatcaaa ctcggtacga ataccaatct gcacggaatg attcgggtcg
     6361 atcagacctt ctttcggcgc ggtatagaac atagtgccgt ggtcaaattc acaaccgttc
     6421 gcataggtat cggtgtgggc gtcaaagtgt accagcgcca ttttgccgaa atgcttcgca
     6481 tgagcacgca gcagcggcag cgtaacaaag tggtcaccac cgaaagagag catacgctta
     6541 ccggcagcca gcagcttctc ggcgtgcgcc tgcagcttct cgctcatctc acgggcatcg
     6601 ccgaaagcat ataccagatc accgcagtcc acgacgttca gacgctcacg catgtcgaaa
     6661 ttccacggga agcggttgtg ttcccaggcc agattcgtcg aaacctgacg gatagctgcc
     6721 ggaccgtggc gaccacccgc acgaccagaa gtggccatat cgaacggcac gccagtaatc
     6781 acccagtcag catcgctgtc atatggctgg aagttcatcg gcaggcgtaa aaaaccaaag
     6841 gcgttggaaa ccagtgagtt atcgtattga tgacctaagg tgctcatgga tggactccta
     6901 ttacaaagtc gatataaaaa aacccttccg cgtcgttagg cccgacgagg aagggttgga
     6961 tttgcgaaac acaagtttgt gctaattatc gccttaaatg ggcgaggcat caagcgaggt
     7021 tgctatttcc gacacggtgg cggcgcatag ccattgttat ctgccggaaa ggcttgtcca
     7081 gagcacggcg gcagcgcgtt ttgcgccggg ccttgtaatt caaaggcggc aaagccgatt
     7141 accccggtat tacgcacatc gccttgcgcc gaattggctg cgtaggaatc agcaacgttg
     7201 gcgaactgaa aagccgcttc agtatgttta tctttgcgga atcctttaat cgctaacgaa
     7261 tctccggcat tcacaatata gccgttgtta ttcagcgaac cttgcttgcc gttcagcaca
     7321 tccaggccat caaccgtagc aacgatctcg tagttggtgt tacgactgta attacgaaca
     7381 tataattgat aattcattcc gactctggcg tggacaatat agcgtggttc attattggat
     7441 gaatcataaa cacgcgtaat aggtagagaa ttaaaattac catcgcgaat agcatattcc
     7501 agttcgttaa tacgaatgct ataaacgcgg tcgtagccgg aaggaacatt agtcgaataa
     7561 ctaataacga tcatctccgc cggatcgcgc caggctcgct cagcagatac gccctgaacc
     7621 gttgagtgca catccccacc ccaggcggtg ccgacattac ccgatgcact gctattatac
     7681 tgcgcattac tggaacaacc ggccagcagc acgattaatg ccaccaacgc gctacgtact
     7741 ttccattttt tcataaccgt tccctctgca tttaattaat gcagcgattg tagggagatt
     7801 atttaataag aaaagcagct aaattatttt taagaatata taaaaagaaa gcggaccgat
     7861 ttacgtaaat aaatcgcccc gccagcagaa taatcttaat tattcatctt caagataggt
     7921 ataaccgtac aaacctgcct cgaactcttc aaggaactgt tgttgcagtt cagcatcaag
     7981 atcggttttc ttcacttgat cacggaactg agttaacagc gttttcggat cgagctgtac
     8041 atattgcagc atgtccgcca cggtatcgcc ttcgtcagac agttccactt cgacactacc
     8101 gtcagggaac acgaacacgt caaccgcttc ggtatcaccg aacaggttgt gcatgttgcc
     8161 gaggatctcc tgatatgcgc cgaccataaa gaaaccgagc atcggcggat tctctggatc
     8221 gtactccggc ataggcattg tcgtggcaat accgtcacca tcaatatagt ggtcgatagc
     8281 accgtcagag tcacaggtaa tatccagcag cacagcgcgg cgttccggca cttgatccag
     8341 cccttccagc ggcagaaccg ggaacaactg gtcgatcccc cacgcatccg gcatcgactg
     8401 gaacagcgag aagttgacgt acattttgtc cgccatacgt tcctgcagct cgtcgataat
     8461 cggacgatga gcacggtttt gcggatccag ctgcttttgc acttcatggc acatgctcaa
     8521 ataaagctgc tcagcccatg cgcgttcttg caggctaaag gtgccggaag agtagccgat
     8581 atgaatgtcg tgcagatcca tctgactgtc gtgtaaccat tcacgcagag aacggcgagt
     8641 tcccggttcg tgcatctcct gccaggtttc ccacatgctt tgcagcgcac gcggcgcatc
     8701 ttctgcaggc gcggtcggca ccgtgtattc gttacgttcc acgccgatga tattagacac
     8761 cagcacggtg tgatgcgcag tcactgcacg acccgattca gtgattaccg tcggatgcgg
     8821 cagaccattt tcttcacacg catcgccaat cgcccagatg atgttgttgg cgtattcatt
     8881 gaggccatag tttacggagc agtcagactg cgaacgggtg ccttcatagt ccacgcccag
     8941 accgccgccg acgtcgaagc actgaatatt gacgcccagc ttgtgcagtt ccacatagaa
     9001 acgcgccgat tcacgaacgc ctgtggcgat atcgcgaata ttcgccatct gcgaaccgag
     9061 gtggaagtgc agtagttgca ggctgtcgag acgcccggct tcacgcaggg tttcaaccag
     9121 ttgcagtacc tgagtcgcag ccaggccgaa cttcgatttt tccccgccgg aggactgcca
     9181 tttacccgag ccctgcgaag ccagacgtgc acgcacgccc agacgaggaa cgacattcag
     9241 acgttctgct tcatccagca caatggcgat ttctgacatc ttctcaatga ccagatagac
     9301 cttgtgcccc atcttctcgc caattaatgc caggcggata tattcgcggt ctttataacc
     9361 gttgcagacg atgacgctac gggtcatgcc agcatgcgcc agcaccgcca tcaactctgc
     9421 tttcgagcct gcttccagcc ccaacggttc gccagaatga atcagggact caatcacgcg
     9481 gcggtgctgg ttaactttga tcggataaac aaggaagtaa tcgccgttat agccgtagga
     9541 ttcccgcgca cgtttgaacg cggcgttaat ggaacgcaaa cggtgctgca ggatctgtgg
     9601 gaaacagaac agtgcaggca gacgctgacc ctgtgcttca cgagttttca ctaactgcgc
     9661 gagatcgacg cgagcttccg ggacgtccgg gtccgggcac acgctgatgt ggcccagctc
     9721 gttaacgtca tagtagttat tgccccacca ggcaatattg taagtacgca gcatcttgct
     9781 ggcttcctgg gagctcattg caacctcctg catggagcgt agtacaccgt gttcgcccgc
     9841 tgacgaaggc aaacccatag acatgtcgtc agacatagcg aacctcaaat tattttatta
     9901 agtgtaaaac agttaacgac tatcgcagcc tggaactgcg ataacaaccc ataagcacat
     9961 ggattttcca gcagtgaatg ctgacgctcc aactgcgcaa ccggtttctt tttcataaca
    10021 ttattaagca cataaccgaa cgtaagtgtg acagttcggc gaaaccacga gaaaactctt
    10081 gtttttacaa gagcgccctt gttcagtcct cagtaactgt aaccagctct tgaatcctga
    10141 gaagcgccga gatgggtata acatcggcag gtatgcaaag cagagatgca gagtgcgggg
    10201 aacgaatctt caccagaacg gtgagacagg ttaagcagca gacaacggtt cattatttcg
    10261 tatcacctcc acggccgcct gttaagacga acccacaagc caaaactctg atttcaaccc
    10321 ggctggaagt ggcaacacga aagaaacgtc gtgtgctttt tatttaagcc gcgcgccgcg
    10381 ttttataccc cacaatggca gaaaattgca aaagataaat acgcagaatg ccggcattgt
    10441 caggaaaaat ttccagccac gtttttaaca gaatgagaca cgattcaaaa aaaagtggaa
    10501 atcgggtgaa gaattgacct aaaatagcca tccagatgtt aatccatcca taccgattaa
    10561 cactcagact gccagtgttt ttaacttgca gagtcgtggt aggatccgct accacagaaa
    10621 atccacacaa cagtttgagc taaccaaatt ctctttaggt gatattaaat atggcaaaac
    10681 acctttttac gtccgagtcc gtctctgaag ggcatcctga caaaattgct gaccaaatct
    10741 ctgatgccgt tttagacgcg atcctcgaac aggatccgaa agcacgcgtt gcttgcgaaa
    10801 cctacgtaaa aaccggcatg gttttagttg gcggcgaaat caccaccagc gcctgggtag
    10861 acatcgaaga gatcacccgt aacaccgttc gcgaaattgg ctatgtgcat tccgacatgg
    10921 gctttgacgc taactcctgt gcggttctga gcgctatcgg caaacagtct cctgacatca
    10981 accagggcgt tgaccgtgcc gatccgctgg aacagggcgc gggtgaccag ggtctgatgt
    11041 ttggctacgc aactaatgaa accgacgtgc tgatgccagc acctatcacc tatgcacacc
    11101 gtctggtaca gcgtcaggct gaagtgcgta aaaacggcac tctgccgtgg ctgcgcccgg
    11161 acgcgaaaag ccaggtgact ttccagtatg acgacggcaa aatcgttggt atcgatgctg
    11221 tcgtgctttc cactcagcac tctgaagaga tcgaccagaa atcgctgcaa gaagcggtaa
    11281 tggaagagat catcaagccg attctgcccg ctgaatggct gacttctgcc accaaattct
    11341 tcatcaaccc gaccggtcgt tttgttatcg gtggcccgat gggtgactgc ggtctgactg
    11401 gtcgtaaaat tatcgttgat acctacggcg gcatggcacg tcacggtggc ggagcattct
    11461 ctggtaaaga tccatcaaaa gtggaccgtt ccgctgcgta cgcagcacgt tatgttgcaa
    11521 aaaacatcgt tgctgctggc ctggccgatc gttgtgaaat tcaggtttcc tacgcaatcg
    11581 gcgtggctga accgacctcc atcatggtag aaactttcgg tactgagaaa gtgccttctg
    11641 aacaactgac tctgctggta cgtgagttct tcgacctgcg cccatacggt ctgattcaga
    11701 tgctggatct gctgcacccg atctacaaag aaaccgcagc atacggtcac tttggtcgtg
    11761 aacatttccc gtgggaaaaa accgacaaag cgcagctgct gcgcgatgct gccggtctga
    11821 agtaatcttt cttcacctgc tttcaaaggc cagcctcgcg ctggcctttt tcttttacct
    11881 caccgcggtg gataggcatt cacgccgcat ccggcaaaaa gctgcccgca caataacatc
    11941 attcttcctc aacacgtttt atcgcaaatt atcatcacta ctgaaaccga ttacaccaat
    12001 aacaccaacc aaatatttgt aatattttca tattattatt cagttttcac agttgttaca
    12061 tttcttttca gtaaagtctt aattgcagat aacagcgttt aatctatgat gatataactc
    12121 aattattttc atgcacttaa accataacta agataaatgt tagtgtaagc gattacactg
    12181 atgtgatttg cttcacatct ttttacgtcg tactcaccta tcttaattca caataaaaaa
    12241 taaccatatt ggagggcatc atgcctgacg ctaaaaaaca ggggcggtca aacaaggcaa
    12301 tgacgttttt cgtctgcttc cttgccgctc tggcgggatt actctttggc ctggatatcg
    12361 gtgtaattgc tggcgcactg ccgtttattg cagatgaatt ccagattact tcgcacacgc
    12421 aagaatgggt cgtaagctcc atgatgttcg gtgcggcagt cggtgcggtg ggcagcggct
    12481 ggctctcctt taaactcggg cgcaaaaaga gcctgatgat cggcgcaatt ttgtttgttg
    12541 ccggttcgct gttctctgcg gctgcgccaa acgttgaagt actgattctt tcccgcgttc
    12601 tactggggct ggcggtgggt gtggcctctt ataccgcacc gctgtacctc tctgaaattg
    12661 cgccggaaaa aattcgtggc agtatgatct cgatgtatca gttgatgatc actatcggga
    12721 tcctcggtgc ttatctttct gataccgcct tcagctacac cggtgcatgg cgctggatgc
    12781 tgggtgtgat tatcatcccg gcaattttgc tgctgattgg tgtcttcttc ctgccagaca
    12841 gcccacgttg gtttgccgcc aaacgccgtt ttgttgatgc cgaacgcgtg ctgctacgcc
    12901 tgcgtgacac cagcgcggaa gcgaaacgcg aactggatga aatccgtgaa agtttgcagg
    12961 ttaaacagag tggctgggcg ctgtttaaag agaacagcaa cttccgccgc gcggtgttcc
    13021 ttggcgtact gttgcaggta atgcagcaat tcaccgggat gaacgtcatc atgtattacg
    13081 cgccgaaaat cttcgaactg gcgggttata ccaacactac cgagcaaatg tgggggaccg
    13141 tgattgtcgg cctgaccaac gtacttgcca cctttatcgc aatcggcctt gttgaccgct
    13201 ggggacgtaa accaacgcta acgctgggct tcctggtgat ggctgctggc atgggcgtac
    13261 tcggtacaat gatgcatatc ggtattcact ctccgtcagc gcagtatttc gccatcgcca
    13321 tgctgctgat gtttattgtc ggttttgcca tgagtgccgg tccgctgatt tgggtactgt
    13381 gctccgaaat tcagccgctg aaaggccgcg attttggcat cacctgctcc accgccacca
    13441 actggattgc caacatgatc gttggcgcaa cgttcctgac catgctcaac acgctgggta
    13501 acgccaacac cttctgggtg tatgcggctc tgaacgtact gtttatcctg ctgacattgt
    13561 ggctagtacc ggaaaccaaa cacgtttcgc tggaacatat tgaacgtaat ctgatgaaag
    13621 gtcgtaaact gcgcgaaatc ggcgctcacg attaatctcc tcaggcttcc tcccgtcgcg
    13681 gaggaagcca cctcttgcag tcatcttttc ttcgctctat cctctgccgc tatgaaaaca
    13741 tcccgtctcc ctatcgccat ccaacaggcc gttatgcgcc gcctgcggga aaaactcgcc
    13801 caggccaacc tgaagctagg gcgtaactac ccggagccaa aactctctta cacccagcgc
    13861 ggaacctccg ccggaacggc ctggctggaa agctatgaaa ttcgcctcaa tcccgttttg
    13921 ctgttggaaa acagtgaagc ttttattgaa gaagtggtac cgcacgaact ggcacatttg
    13981 ctggtatgga aacatttcgg ccgcgtagcg ccacatggca aagagtggaa gtggatgatg
    14041 gaaagcgtgc tgggtgttcc cgcccgtcgt acgcatcagt tcgaactgca atccgtgcgt
    14101 cgcaacacct tcccctaccg ctgcaagtgc caggagcatc agcttaccgt acgccgccat
    14161 aatcgcgtag ttcgtggcga ggccgtctat cgctgtgttc actgcggtga acagctggtt
    14221 gcgaaataac catctgaact atcaggaact ttcctgatct ggctgattgc ataccaaaac
    14281 aactttcgct acgttgctgg ctcgttttaa cacggagtaa gtgatgtacc gttatttgtc
    14341 tattgctgcg gtggtactga gcgcagcatt ttccggcccg gcgttggccg aaggtatcaa
    14401 tagtttttct caggcgaaag ccgcggcggt aaaagtccac gctgacgcgc ccggtacgtt
    14461 ttattgcgga tgtaaaatta actggcaggg caaaaaaggc gttgttgatc tgcaatcgtg
    14521 cggctatcag gtgcgcaaaa atgaaaaccg agccagccgc gtagagtggg aacacgtcgt
    14581 tcccgcctgg cagttcggtc accagcgcca gtgctggcag gacggtggac gtaaaaactg
    14641 cgctaaagat ccggtctatc gcaagatgga aagcgatatg cataacctgc agccgtcagt
    14701 cggtgaggtg aatggcgatc gcggcaactt tatgtacagc cagtggaatg gcggcgaagg
    14761 ccagtacggt caatgcgcca tgaaggtcga tttcaaagaa aaagctgccg aaccaccagc
    14821 gcgtgcacgc ggtgccattg cacgcaccta cttctatatg cgcgaccaat acaacctgac
    14881 actctctcgc cagcaaacgc agctgttcaa cgcatggaac aagatgtatc cggttaccga
    14941 ctgggagtgc gagcgcgatg aacgcatcgc gaaggtgcaa ggcaatcata atccgtatgt
    15001 gcaacgcgct tgccaggcgc gaaagagcta acctacacta gcgggactct ttttgttaac
    15061 ccctacccca cgcgtacaac cgcgtgggga gacgacgcgg atttttaact atgcgtatcc
    15121 cccgcattta tcatcctgaa ccactgacca gccattctca catcgcgctt tgcgaagatg
    15181 ccgccaacca tatcgggcgc gtactgcgca tggggccggg gcaggcgttg caattgtttg
    15241 acggtagcaa ccaggtcttt gacgccgaaa ttaccagcgc cagcaaaaaa agcgtggaag
    15301 tgaaggtgct ggaaggcaag atcgacgatc gcgaatctcc gctgcatatt cacctcggtc
    15361 aggtgatgtc gcgtggtgaa aaaatggaat ttacgatcca gaaatcgatc gaactcggtg
    15421 taagcctcat tacgccactt ttttctgagc gctgcggcgt taaactggat agtgaacgtc
    15481 tgaacaagaa gctccagcag tggcagaaga ttgcaattgc tgcctgtgag cagtgtggtc
    15541 gtaaccgggt gccggaaatc cgcccagcga tggatctgga agcctggtgt gcagagcagg
    15601 atgaaggact gaaactgaat cttcacccgc gcgccagtaa cagcatcaat acgttgccgt
    15661 taccggttga acgcgtccgc ctgctgattg gcccggaagg cggtttatcg gcagatgaaa
    15721 ttgccatgac tgcccgctat caatttactg atatcctgtt gggacctcgc gttttgcgta
    15781 cagagacaac tgcgctcacc gccattaccg cgctacaagt acgatttggc gatttgggct
    15841 aacggagaag aataatgatc aagctcggca tcgtgatgga ccccatcgca aacatcaaca
    15901 tcaagaaaga ttccagtttc gctatgttgc tggaagcaca gcgtcgtggt tacgaacttc
    15961 actatatgga gatgggcgat ctgtatctga tcaatggcga agcccgcgcc catacccgca
    16021 cgctgaacgt gaagcagaac tacgaagagt ggttttcatt cgtcggtgaa caggatctgc
    16081 cgctggccga tctcgatgtg atcctgatgc gtaaagaccc gccgtttgat accgagttta
    16141 tctacgcgac ctatattctg gaacgtgccg aagagaaagg gacgctgatc gttaacaagc
    16201 cgcagagcct gcgcgactgt aacgagaaac tgtttaccgc ctggttctct gacttaacgc
    16261 cagaaacgct ggttacgcgc aataaagcgc agctaaaagc gttctgggag aaacacagcg
    16321 acatcattct taagccgctg gacggtatgg gcggcgcgtc gattttccgc gtgaaagaag
    16381 gcgatccaaa cctcggcgtg attgccgaaa ccctgactga gcatggcact cgctactgca
    16441 tggcgcaaaa ttacctgcca gccattaaag atggcgacaa acgcgtgctg gtggtggatg
    16501 gcgagccggt accgtactgc ctggcgcgta ttccgcaggg gggcgaaacc cgtggcaatc
    16561 tggctgccgg tggtcgcggt gaacctcgtc cgctgacgga aagtgactgg aaaatcgccc
    16621 gtcagatcgg gccgacgctg aaagaaaaag ggctgatttt tgttggtctg gatatcatcg
    16681 gcgaccgtct gactgaaatt aacgtcacca gcccaacctg tattcgtgag attgaagcag
    16741 agtttccggt gtcgatcacc ggaatgttaa tggatgccat cgaagcacgt ttacagcagc
    16801 agtaacccac cttagcgaga aggatctcgt tgagactctg agtgacagcg cccttctttc
    16861 cacgcatact gggcgctgtt gcttttttga accaggaaac agaacctctg acaatgaatt
    16921 tacagcatca ctttcttatt gccatgcctg ctctccagga tccgattttc cgtcgttccg
    16981 tggtctacat ttgcgaacat aataccaatg gtgcaatggg gatcatcgtc aacaagccgc
    17041 tggaaaatct caaaattgaa gggattctgg aaaagctgaa gatcacgccg gagccgcgtg
    17101 atgaatcaat ccgtctggat aaaccggtta tgctcggcgg tccgctggct gaagatcgcg
    17161 ggtttatttt gcatactccg ccctccaatt ttgcttccag cattcgcatt tcagacaaca
    17221 cagtgatgac cacttcccgc gatgtgctgg aaacgctcgg caccgataaa caaccgtctg
    17281 acgtattggt ggctctgggt tatgcctcct gggaaaaagg tcaactggaa caagaaattc
    17341 tcgataacgc gtggctaacg gccccggcgg atctgaatat tctgttcaaa acgccgattg
    17401 ccgaccgctg gcgcgaggcg gcaaaactga ttggtgtgga tattctcacc atgcctggtg
    17461 tggcagggca cgcctgatga gtggaacctt actcgccttc gacttcggca ccaaaagcat
    17521 tggcgtagcg gtcggccaac gcattaccgg cactgctcgc cctttgcctg caattaaagc
    17581 acaggacggt acgccggact ggaacctcat cgaacgttta ctgaaagagt ggcagccgga
    17641 cgaaatcatc gtcggtttgc cgctgaatat ggacggcacc gagcaaccat tgactgccag
    17701 agcgcgtaaa tttgccaacc gtattcatgg tcgtttcggt gttgaagtaa agctccatga
    17761 tgaacgtctt agcaccgtgg aagcccgttc cggtctgttt gaacagggcg gctatcgggc
    17821 gctcaacaaa ggcaaagtcg actctgcctc tgcggttatt attctcgaaa gctatttcga
    17881 gcagggatat taaggcgatc taaaagcctg gcggatagtc gttaccgcca tcttgccgga
    17941 tgcggcgtga acgccttatc cagtacacat ctggcagcgg ctgtaggtct gataagacgc
    18001 gttagcgtcg catcagacat ttattgcctt tataggccct ataaacttgc gcatcgggca
    18061 tggcaacgtc acaaacgccc ttcccccacc cgctgctgat aactctgctg aaacgttaac
    18121 atccccacct gctgcccggt ttgaataaca tgcggtaact ggtgggtttt cccttcgcga
    18181 atcaaattcc ccaccgcggg agtgttaatc aataattcaa acagcgccac gcgtccttcc
    18241 tgtttatcca cttccagctt ttgtgacaac acggcccgta aactacctgc cagttgatta
    18301 cgtacgggat ctttttcctg cgccggaaat gaatccacca gtcgctcaac tgcctgcgcg
    18361 gcaccacgcg tatgtaatgt tgccagcacc aaatgcccgg tttctgccgc cgtcagtgcc
    18421 agacggattg tctcgctgtc acgcagctct ccgagcaaaa tcacatcagg atcttcccgc
    18481 aatgcggccc gcaatcccga tgcgaacgtc atacagtgca aaccaatttc ccgctgctgg
    18541 atcaaacatc gctggctggc atagatatat tcaacaggat cttccagcgt cagaatatgc
    18601 gcatcggcat gttgattgag atagccaacc atcgccgcca gcgtggtaga tttgccgctc
    18661 cccgtcgccc ccgtcaccag aatcaggcca ttctcgctct tgagtaattc cggcaatacc
    18721 gttggtgcgc caagctgttc gagctgtgga cagtgcgaag gtaacaaccg taatgccaga
    18781 gaaatacctt gccgttgcgc gaacgcactg ccacgcaacc gctggttttc cgccagcgac
    18841 acggcaaaat ccagctggcc attttccagc aatattgccc gctgatcgtc atccagccac
    18901 tcccgcagta gctcttcgac gtccggcgca tcaaacggcg cagcttccat tagcccgcga
    18961 atgcgccatc gtgcgggcca ggcgctgcac aggtgtagat ccgagacgtt atgctttaca
    19021 ctaagggcca caatttcttc catattcata ctaagatcct cggaaaatga acgatattgc
    19081 gcataacctg gcacaggtcc gggacaaaat ctcagcggct gcaacgcgtt gcggccgttc
    19141 tccagaagaa attacgctgc ttgcagtcag taaaacaaaa cctgcgagcg ccatcgcaga
    19201 agccattgat gccgggcagc gtcaatttgg tgaaaactac gttcaggaag gggtagataa
    19261 aattcgccac tttcaggaac tgggcgtaac aggattagaa tggcatttta ttggcccgtt
    19321 gcagtctaat aaaagccgcc tggtggcaga gcatttcgac tggtgtcata ccatcgaccg
    19381 tttgcgcatt gctacccgcc tcaacgatca gcgcccggca gaacttcccc ctcttaacgt
    19441 actgattcaa attaatatta gtgatgaaaa cagtaagtcc gggattcaac tggcagaact
    19501 ggacgagctg gcagcggcgg tcgctgaact accgagttta cgtctgcgcg ggctgatggc
    19561 aatccctgcg cctgagtcag aatatgtaag gcagtttgaa gttgcacgcc aaatggctgt
    19621 agcatttgcc ggactgaaaa cgcgctaccc gcatatcgac acgctctctc tgggaatgtc
    19681 ggacgatatg gaagccgcca ttgcggcagg tagcacgatg gttcgtatcg gcactgcaat
    19741 ttttggtgcg cgtgattact ctaaaaaata aggaattaaa ggaacgccat gaatacgttg
    19801 actttcctgc tttcaacggt cattgagctg tataccatgg tgctgttatt acgcatctgg
    19861 atgcagtggg ctcattgtga tttttacaac cccttctcac agtttgtagt gaaggtaacg
    19921 cagccaatta tcgggccact gcgccgcgtt attccggcaa tgggtcccat tgatagcgcc
    19981 tcgctgctgg ttgcgtacat tctcagtttt atcaaagcca tcgtgctgtt taaagtggtg
    20041 accttcctgc caatcatctg gattgccggt ttactgattc tgctgaaaac catcggcctg
    20101 ctgattttct gggtcctgct ggtgatggcg attatgagct gggtaagcca gggtcgtagt
    20161 ccgattgaat acgtgctgat tcagctggcc gatccgctgc tgcgcccgat tcgccgcctg
    20221 ctaccggcaa tgggtgggat tgatttctcg ccgatgatcc tcgttctgct gctgtatgtc
    20281 atcaatatgg gtgtcgcaga agtattacag gcaaccggaa acatgctact gccggggctg
    20341 tggatggcgt tatgagtgcc gtaacagtta atgatgacgg tctggtttta cggctttata
    20401 ttcagccgaa agccagccgt gattctattg tcggtttaca tggcgacgaa gttaaagtcg
    20461 ccattaccgc gccgccggtt gacggtcagg ccaacagcca tctggtgaag tttctcggta
    20521 agcaattccg ggttgccaaa agccaggtgg tgattgaaaa aggcgaactt ggccgccaca
    20581 aacaaattaa aatcattaat ccgcaacaaa tcccgccaga aatcgcggcg ttaattaatt
    20641 aggtatccta tgcaaaaagt tgtcctcgca accggcaatg ccggtaaagt gcgtgagctg
    20701 gcgtcgctgc ttagcgactt cggtcttgat atcgtggccc aaacagacct cggcgtagat
    20761 tccgctgaag aaaccggcct gacctttatc gaaaacgcga ttctgaaagc gcgccatgcg
    20821 gcaaaagtga ccggtttacc ggcaattgcc gacgactctg gtctggcggt agatgtactt
    20881 ggcggcgcgc cgggcattta ctccgcgcgt tattccggtg aagacgcgac cgatcaaaag
    20941 aatctgcaaa aactgctgga aacactaaaa gacgtaccgg acaaccaacg tcaggcgcgt
    21001 ttccactgcg tactggtata tctgcgtcac gcggaagatc ccactccgct ggtgtgccac
    21061 ggcagctggc cgggcatgat tactcgtgaa ccagcgggta ctggcggctt tggttatgat
    21121 ccaatcttct tcgtaccttc cgaagggaaa accgctgccg aactgacccg cgaagaaaag
    21181 agcgccattt cccaccgtgg tcaggcattg aaactgctgc tggacgcttt acgtaatggt
    21241 taaattaccg ccgctaagtc tctacattca catcccgtgg tgcgtgcaga aatgcccgta
    21301 ctgcgatttc aactctcacg cgttgaaagg agaagtgccg cacgacgatt atgttcagca
    21361 tctgcttaac gacctggaca acgatgtggc ttacgctcag ggccgtgaag taaagacaat
    21421 ctttattggc ggtggtacgc cgagcctgct ttccggcccg gcgatgcaaa cgctgctgga
    21481 cggcgtgcgt gcgcgtttgc cgctggcagc ggatgcagaa attactatgg aagcgaaccc
    21541 aggcacggta gaagcggatc gctttgtcga ttatcagcgt gctggtgtga accgcatctc
    21601 tattggtgtg cagagtttta gcgaagaaaa gctgaaacga cttgggcgta ttcatggccc
    21661 gcaagaagcg aaacgagcgg caaagctggc gagcggttta gggttacgta gctttaacct
    21721 tgatttgatg catggactac cggatcaatc actggaagag gcgcttggcg atctacgcca
    21781 ggccattgaa ctgaatccgc cgcatctttc ctggtatcaa ctgaccatcg aacccaatac
    21841 gctgtttggt tcgcgaccac cggtgctgcc ggacgacgat gcgttgtggg atattttcga
    21901 acaggggcat cagttattaa ccgcagcggg ttatcagcaa tatgaaactt ccgcttacgc
    21961 caaacccggt tatcagtgcc agcacaatct caactactgg cgctttggcg actacatcgg
    22021 tattggctgc ggcgcgcacg ggaaagtgac cttcccggat gggcgcattc tgcgtaccac
    22081 caaaacgcgt catccgcgtg gttttatgca aggaaggtat ctggaaagcc agcgtgatgt
    22141 cgaagccgca gataagccgt ttgagttctt tatgaatcgc ttccgtctgc tggaggccgc
    22201 gccgcgcgtg gagtttattg cgtataccgg gctttgcgaa gatgtgattc gcccacagtt
    22261 agacgaggcg attgcccagg gttatctcac agaatgtgcg gattactggc agataacgga
    22321 acatgggaag ctgtttttaa attcgctgct ggagcttttt ctggctgagt aaacttgtat
    22381 tgccggatgc ggcgtgaacg ccttatccag cctacatgtg gcaacggttg taggtctgat
    22441 aagacgcgca agcgtcgcat cagacgttga ttgccggatg cggggtcaac gccgcatcct
    22501 gctacaaatc gtgcactata tcaaacttac ttaatcaccc cattgcgccg cagttgttcg
    22561 gtatcttttt tccagcgttc attagcttgg ttgatatcgt caattttcat caaaatcgcg
    22621 acttttttac tgtttatttc tgaaactttc atcgttaacg gatattgccc tttttcgagc
    22681 gttacccagt catcctgcaa tttacggctg agtttttgtg attgcagaaa agtctgtcgc
    22741 cgttgttcgt tgatatcgaa atctcgtttc tgctgaatcg gcacttcata acactcttca
    22801 ctctgtttat gacatgccgc aatcgccggt tgataaacct ctttgtcata tttaaccgcg
    22861 aacgcctcgc tatcgttctg tttattaaac tcatcacgct gttggtgaat ttttttaaag
    22921 gcttcttcgc gggtcatttg cttaccgttt gcatcttttc cccaatatgc atccgctgct
    22981 ttctgcgccg ccaccatttg cttatcaagc gctttcactt ccttttccag cgcaggaatc
    23041 gagttcttct gggtattcaa cgcagattca agctgactaa agcgataatt gaaatcttcg
    23101 ctattcacga tcagatattt attattcgtt ttgatatcgt cgaccacccg cccacgatcg
    23161 ctggctgccg cctgaaaaga gtaaaagttc accgaccagc cagacgcggg cgttccttta
    23221 ctggttaaca tcgccgagaa ttttaccggt ttatctttcg tccaggtctg ttcgagcagt
    23281 tcgtaatctg ccaactgacc gacccaggta tagagatcgt cactggaaga gacatcgcct
    23341 tccgctgacc aggtggcctg attaccctta gcatcaagat tttttaaggt gatagcatca
    23401 agcttgagaa tgccgtgata ctgctttttg aattgatctt ttaagatatt ttcagttggc
    23461 ggttcgccat ctgcccaggc attaccagtc atcaacataa gcagtgccgt gccaacaatc
    23521 cattgctttt tcacccgaaa aacctcacgc ggaataattt attccggtga ttatcattag
    23581 ggtaatgaaa attggcaatg gacgtaagtc tgaagtgaaa aagccccggc acgataccgg
    23641 ggcgaggtga ttagtactga ttgaagatct gctggatctg ctgcggatct ttggtttgcg
    23701 tcagagccag ttgcagcaga acgcgcgctt tttgcgggtt cagcgtgcca gaggcgacga
    23761 agccgtattt cgcatcatcc acttcggcat cctgagtggt agcgcccgtc ggtacgcggg
    23821 aagaacgcac gactgcagta ccgtttttcg cggcggtcgc cagcgtgtcg aacacagatt
    23881 tatacaggtt gccgttaccc acaccagcgc taacgatgcc atcatagccc gcatctacca
    23941 gtgctttagc cggaagatcg gatgcgttag cgtagttata aacaatgccg actttcggca
    24001 gttcattcag cttagagaca tcgaatggcg tgtcgctggt atgcttacgt gccggggtac
    24061 gctggtagtc aatcttaccg ttgtgaatgt aacccagagg accgtagtta acagacttga
    24121 aggtcgctac gtcggtggtg ttggttttgg tgacgtcacg gccatcaagc acggtgtcat
    24181 tcatcactac cagcacgcca cggttggcgg aggctttatc agctgcggtc actaccgcgt
    24241 tatacaggtt gaatggaccg tctgcgctca tagacgtgga cggacgcatt gcgccgacca
    24301 tcaccaccgg tttgtcgcat ttcaccgtca ggtcgaggaa gtaagcagtt tcttccatcg
    24361 tgtcggtacc gtgggtaatg acgaagccgt cggtcttatc gcagtcggtg ttaatttttt
    24421 tcgccagtgt cagccagaca ttatcgttca tgtcctggga gccgatattc actacctgct
    24481 cgcctttaac gttcgcaatg tccttcagtt gcggcaccgc attaaccaga ttttctacgc
    24541 caactttacc cgctgtgtag ttagatttgg ttgcggagtc accaccaccg gcaatggtcc
    24601 cgccggttgc taaaatggtg atattgggta atgccaatgc tgcaccacta aaacccataa
    24661 ccagtgcggc aagtgccgtc tttttgaaaa actccatttc attcctccag ttacgtgaac
    24721 gctacgcatt atcccttagc tttgtatggg aaatttgacg ttaaacaatt tacaacgtga
    24781 atatattttg gagatctaca aagttagagg caggtaacaa aacgaagaat taaacggcat
    24841 aaaaaagtat tatgccgtct taaaatagag aattatttta aattcccgac cagggctttg
    24901 cggctatctt ccagagtcac aacacggcta caaacatctt tgccaaactg ctggaaatct
    24961 ttttcctgct ttttccactc ggtttggatt gaggattgca gcccccccag gcttcccagc
    25021 acattctgta atgggttacc gccgcttttc agcaccgctt tcgcgcccat ttcattaatg
    25081 ctgtcctgta aaattccgcc cattgcctga ttcactaatt gctggccttc ggcgcgaacc
    25141 tgatcaatgg ctttatagtg aaacgtcagg ccatcgctgc gcgtttcgat aatgcggttc
    25201 atctgctctt tcagctgcgc atcaagtttg gtcagacggc tgcgcatttt gctgctttcg
    25261 cccatctcct gaacgataat tttatccaga gcaatacggg ctttctcgac gcggcttttc
    25321 gcgccttcat caatccacgg cagcgtgcta cgtagttcag cctgataatc cttcgcctgc
    25381 tcgcgctggg cggcattcag ggaatattgc ttaccgttat acatcacgtt gccgtctggc
    25441 gtgatcacca gattgccgtt ttcgcccttc acctgcacgg tttgcgggct gacaatcaca
    25501 tcgtcacgcg gcgtgacgct gcactggtag tcggcgtgag cggtcattgc cgtcactgaa
    25561 agtgctgccg ccagcagcat tttgcgcatc atagtcttcc ctcaagaaca aatcaggcca
    25621 gcatttgctg gccccagatt gataacaaag tacgcgtttt ccatgccgga tgcggcgtaa
    25681 acgccttatc cggcctactc gatcgtgcat ctttccaggc atgataagcg tagcgcatca
    25741 ggcagttttg catttgtcat cgcccgtatg ctttcttagt cccaccaaac gtcgaaaagt
    25801 tcgctggtgc gtacctcatc cagtttgcgc tcttccagcc acttacgcac aattgcctga
    25861 tgttcttcgg tgcatttgcc gatttcctgc atgcagatca gaccttccca ggccagataa
    25921 ccgctgccgt caaaggccag tttgttcggt tcgataacct cgttaataaa atcatcaacg
    25981 gttttatcaa tctgttcttc cgatgtacct tccgggaatc gccatgccac cgaaaatcct
    26041 aattcctgga attcgtcgat gtgcattttt ttacgcagac gacggctacg gttctttgcc
    26101 attatttcac cctctcgaac attaagtccc atactccgtg accaagacga tgaccacgtt
    26161 gttcaaattt cgtcactgga cgtgatgccg gacgcggtac gtaatcattg ctctctgaca
    26221 ggtttttata accgtcaata gaagacatca cttcaagcat atgttccgca taaggttccc
    26281 agtcggtcgc catatggaat acgcccccca gctgcagttt gctttttacc agttcggcaa
    26341 acggcacctg aacgatacgg cgtttattat ggcgcgcttt gtgccacggg tcagggaaaa
    26401 agagctgcac catgcgcaat gaattgtcag gaatcatttt atgcagcact tcaaccgcat
    26461 cgtgacacat cacgcgcagg ttgcttaagc cctcttcatg cgcagaagcc aggcacgcac
    26521 caacgcccgg tgaatgcact tcaatgccga ggaagtcctg ctcggggcga tctttagcca
    26581 ttgccaccag cgacgccccc atgccaaaac caatctcaag cgtcaccggc gcttcacggc
    26641 caaaaagcgc ggggaaatcc agcatatctt cgctgaactc aacgcccatc accggccagt
    26701 agttttccag cgcatgttcc tggcctttgg tcagtcgccc ctggcggcgc acaaaactac
    26761 ggatacggcg cagtgggcgg ccgttttcat caaattccgg tgaaatgacg tcgtttttca
    26821 taaagattta gtcgcttgtg aaagtgttct gaaaacgggc attatccaaa gttagttgcc
    26881 ggatgcaagc atgataaggc cgtggctgcg gaaagttccg gtttacaccc tgccgtcgct
    26941 gtgctgcaat cttgccccca acaacagtga attcggtgac catgcaagcg tcgcaatttt
    27001 cagcccaggt tctggactgg tacgataaat acgggcgaaa aactctgccc tggcaaattg
    27061 acaagacgcc ctacaaagta tggctctcag aagtgatgtt gcaacaaact caggttgcga
    27121 ccgttatccc ctattttgaa cgctttatgg cgcgcttccc gacggtgacc gatctcgcca
    27181 atgcgccgct cgacgaagtt ctccacttgt ggaccgggct tggctattac gcccgcgcgc
    27241 gcaatctgca taaagcggca caacaagtgg cgaccttaca cggcggtaaa ttcccggaaa
    27301 cctttgagga agttgcagca ctgccgggcg tcgggcgttc caccgcaggc gcgattctct
    27361 cgctttctct gggtaagcac tttccgattc tcgacggtaa cgtcaaacgc gtgctggcgc
    27421 gctgctatgc tgtaagcggc tggcctggga aaaaagaggt cgagaataaa ttatggagtt
    27481 taagcgagca ggtgacgccc gcggtcggcg tggaacggtt taatcaggcg atgatggatt
    27541 tgggcgcgat gatttgtacg cgctcgaaac cgaaatgttc gctctgtccg ctacaaaacg
    27601 gatgtattgc cgccgccaac aatagctggt cgctttatcc gggcaaaaaa ccgaaacaga
    27661 cgctgccgga gcgcaccggc tactttttgc tattacagca cgaagatgaa gtattgctgg
    27721 cgcagcgtcc gccgagcgga ttgtggggcg gtttatactg tttcccgcag tttgccgacg
    27781 aagaaagttt gcggcattgg ctggcgcaac ggcagattgc tgccgataac ctgacgcagc
    27841 tgaccgcgtt tcgccattcc ttcagccatt tccacttaga tattgtgcct atgtggcttc
    27901 ccgtgtcgtc attcaccggc tgcatggatg aaggcaatgc gctctggtat aacttagcgc
    27961 aaccgccgtc agttggcctg gcggctcccg tggagcgttt gttacagcag ttacgcactg
    28021 gcgcgccggt ttagcgcgtg agtcgataaa gaggatgatt tatgagcaga acgatttttt
    28081 gtactttcct gcaacgtgaa gcagaaggtc aggattttca gctgtacccc ggcgagctgg
    28141 gaaaacgcat ctataacgag atctccaaag aagcctgggc gcagtggcag cacaagcaaa
    28201 ccatgctgat taatgaaaag aaactcaaca tgatgaatgc cgagcaccgc aagctgcttg
    28261 agcaggagat ggtcaacttc ctgttcgagg gtaaagaggt gcatatcgag ggctatacgc
    28321 cggaagataa aaaataaaaa cagtgccgga gcacgcctcc ggcaacttgc ataaaaacaa
    28381 acacaacacg cacccggaat gatgaaaaaa tatctcgcgc tggctttgat tgcgccgttg
    28441 ctcatctcct gttcgacgac caaaaaaggc gatacctata acgaagcctg ggtcaaagat
    28501 accaacggtt ttgatattct gatggggcaa tttgcccaca atattgagaa catctgggga
    28561 ttcaaagagg tggtgatcgc cggtcctaag gactacgtga aatacaccga tcaatatcag
    28621 acccgcagcc acatcaactt cgatgacggt acgattacta tcgaaactat cgccgggaca
    28681 gaacctgccg cgcatttgcg ccgggcaatt atcaaaacgt tgctgatggg tgacgatccg
    28741 agttcggtcg atctctattc cgacgttgat gatattacga tttcgaaaga acctttcctt
    28801 tacggtcagg tggtggacaa caccgggcag ccgattcgct gggaaggtcg cgcaagcaac
    28861 ttcgcggatt atctgctgaa aaaccgtctg caaagccgca ataacgggct gcgtatcatc
    28921 tacagcgtca ccattaacat ggtaccgaac caccttgata aacgtgcgca caaatatctc
    28981 ggcatggtcc gccaggcgtc acggaaatat ggcgttgatg agtcgctgat tctggcaatt
    29041 atgcagaccg aatcttcctt taacccgtat gcggtcagcc gttccgatgc gctgggatta
    29101 atgcaggtgg tacaacatac tgccgggaaa gatgtgttcc gctcgcaggg gaaatccggc
    29161 acgccgagcc gcagtttctt gtttgatcct gccagcaata ttgataccgg caccgcgtat
    29221 ctggcgatgc tgaacaatgt ttatctcggc ggaattgata acccaacatc gcggcgttat
    29281 gccgtcatca ccgcctataa cggcggcgca ggcagcgtgc tgcgagtctt ttcgaatgat
    29341 aagattcagg ctgccaatat tattaacacc atgacgccgg gcgatgttta tcagacgctg
    29401 acgacccgcc atccctctgc ggaatctcgc cgttatcttt ataaagtgaa taccgcgcaa
    29461 aaatcctacc gccgccgata attccattaa ccgcccctga cgatgctcag gggcaaaaat
    29521 gttatctaca tcacaatttc gttttgcaaa ttgggaatgt ttgcaattat ttgccacagg
    29581 taacaaaaaa ccagtccgcg aagttgatag aatcccatca tctcgcacgg tcaaatgtgc
    29641 tttttcaaac actcatccgc atcacgatgt gaggaaatta acatgaatct taagctgcag
    29701 ctgaaaatcc tctcttttct gcagttctgt ctgtggggaa gttggctgac gaccctcggc
    29761 tcctatatgt ttgttaccct gaagtttgac ggtgcttcta ttggcgcagt ttatagctca
    29821 ctgggtatcg ccgcggtctt tatgcctgcg ctgctgggga ttgtggccga caaatggtta
    29881 agtgcgaaat gggtatatgc catttgccac accattggcg ctatcacgct gttcatggcg
    29941 gcacaggtca cgacaccgga ggcgatgttc cttgtgatat tgattaactc gtttgcttat
    30001 atgccaacgc ttgggttaat caacaccatc tcttactatc gcctgcaaaa tgccgggatg
    30061 gatatcgtta ctgacttccc gccaatccgt atctggggca ccatcggctt tatcatggca
    30121 atgtgggtgg tgagcctgtc tggcttcgaa ttaagccaca tgcagctgta tattggcgca
    30181 gcactttccg ccattctggt tctgtttacc ctgactctgc cgcatattcc ggttgctaaa
    30241 cagcaagcga atcagagctg gacaaccctg ctgggcctcg atgcattcgc gctgtttaaa
    30301 aacaagcgta tggcaatctt ctttatcttc tcaatgctgc tgggcgcgga actgcagatt
    30361 accaacatgt tcggtaatac cttcctgcac agcttcgaca aagatccgat gtttgccagc
    30421 agctttattg tgcagcatgc gtcaatcatc atgtcgattt cgcagatctc tgaaaccctg
    30481 ttcattctga ccatcccgtt cttcttaagc cgctacggta ttaagaacgt aatgatgatc
    30541 agtattgtgg cgtggatcct gcgttttgcg ctgtttgctt acggcgaccc gactccgttc
    30601 ggtactgtac tgctggtact gtcgatgatc gtttacggtt gcgcattcga cttcttcaac
    30661 atctctggtt cggtgtttgt cgaaaaagaa gttagcccgg caattcgcgc cagtgcacaa
    30721 gggatgttcc tgatgatgac taacggcttc ggctgtatcc tcggcggcat cgtgagcggt
    30781 aaagttgttg agatgtacac ccaaaacggc attaccgact ggcagaccgt atggttgatt
    30841 ttcgctggtt actccgtggt tctggccttc gcgttcatgg cgatgttcaa atataaacac
    30901 gttcgtgtcc cgacaggcac acagacggtt agccactaat tacgcaaaga aaaacgggtc
    30961 gccagaaggt gacccgtttt ttttattctt acttcaacac ataaccgtac aaccgtttca
    31021 tgccatccgc atcggtttcg ctataaacac cttgcagctc cggcgaaaat cccggcaaca
    31081 aattcacccc ttcttccagt gcaaggaaat aacgttgaac cgccccaccc cagacttccc
    31141 cgggtaccac gcaaagcacg ccaggtggat aaggcaacgc cccttctgcc gcaattcgcc
    31201 cttcggcatc acgaatccgc accaactcca cttcaccgcg aatataagcg ctatgcgcat
    31261 cctgggggtt catcaccact gacgggaaac tctgctggcg gaacatcgct ttttgtaggt
    31321 ctttgacgtc gaaactgaca tacagatcgt gcatctcctg acacaactgg cgcagggtgt
    31381 agtcgcgata gcgcaccgga tatttgttat aaacgctcgg caacacctca gccagcggcg
    31441 agtcatcctc aatatgctgt tcaaattgcg ccagcatcgc caccagttgt gccagcttct
    31501 cgtggctttc cgccggagtt aataagaaca gaatggagtt gagatcgcac ttctccggca
    31561 caatgccgtt ctcacgcaga tagtgcgcca gaatcgtcgc cggaacgcca aagtcgctat
    31621 attcgccagt ttcggcattg atacctggtg tagtgagtaa cagcttgcac ggatcaacaa
    31681 aatactgatc cgcggcatat ccttcaaagc cgtgccactt cgcccccggc tcaaaactga
    31741 aaaaacggcg gtcgctggct aacacagatg tcggataatc ctgccacaat ttgccatcaa
    31801 caacgggcgg gataaacggg cggaacagct tacagcgcgc aagaatagcc ttgcgcgact
    31861 caatacccaa ctcaacgcac tcagcccaca gccgacgccc actctcccct tcatgaattt
    31921 tggcgttaac atccagtgca gcaaacagcg gatagaaagg gctggtagaa gcatggagca
    31981 taaaggcgtt attcaaccgc ttatgcgggc aaaaacgcgc ctgtccgcgg atatggttat
    32041 cttttttatg gatctgcgac gtctgtgaga atccagcctg ctgtttgtgc accgactggg
    32101 tcacaaaaat ccccggatcg ttttcgttaa gttctaacag cagcggcgag ctatccgcca
    32161 tcatcgggat aaattgttcg taaccgaccc acgcggaatc aaacagaatg taatcacaca
    32221 gatgcccaac ggtatcgatc acctgacggg cgttatagac agttccgtca taggttccca
    32281 gctgaataat cgccaggcga aacgggcgcg gcagctcggc tttttctggc gcaacgtcgc
    32341 gaatttgctg gcgaagatac tcttcattaa aacagtgcgc atcaataccg ccaatgaaac
    32401 caaacgggtt gcgtgaagct tccagataga ccggcgtcgc ccccgcctga atcagcgcgc
    32461 cgtgatgatt cgacttatgg ttgttacggt cgaagagcac cagatcgcca cgcgttaaca
    32521 gcgcattcgt caccacttta ttcgctgccg atgtgccgtt cagcacaaaa taggttttat
    32581 cggcatgaaa gactttggct gcgaatttct gcgcatcttt cgccgatcct tcatgaataa
    32641 gcagatcgcc caattttacg tcagcgttac acatatcggc gcgaaagacg ttctcaccaa
    32701 agaaatcgta aaaatggcgt ccggcaggat gctttttaaa aaacgcacca tgttgatgtc
    32761 cagggcaagc aaaggtgctg ttgcccatct caacgtactg cgtcagcgtg tcataaaacg
    32821 gtggcagcaa attctcttca tactgacagg ctgcggattc cagctccagc cactgctgct
    32881 cgttgccgtt gattaccgcc gtaacgcccg caggtaattc aacagcatgt tcggaataca
    32941 aaaacaccgg tagatgaaaa ccggtgcgct taagcaacgc aagaatgcca ctgcgactat
    33001 ccgcagcggt aatgacgact gccgcgacgt ccgtaaaatc agtatctccc aacgccacca
    33061 cgcgacgatg agaagaaagt cgggatacca gttcactact ggcggcaata ttcattgatt
    33121 tcataagcgc aaacccgttt cggggaagta agaataccgg acaaggtgga aaaccctgcc
    33181 ccatgagata tgggtcaaac tggtcaccag ctccgaccgc cagacatcag taaaagcaga
    33241 aacgctctga ttttactgtt gtcctgcagt gagcgtgcgt taacttcacc gcatgagcag
    33301 taacatagaa agggaaacgt ttcgcgcgaa atggcgataa gcgagagaat gtaaggagat
    33361 ggcgtgcatc gggcaaactc cgtacagaga ggagaaaatt cgcgcaatca tggcaccttt
    33421 cgctaaagcg tgcaagccag gactttgcga ccagacaagc catcaacaag gcaaaaagtt
    33481 cataataaga aaatcaaaca atacagctaa caggaactct tgtggtcatc ggccctttta
    33541 tcaacgctag tgcagtctta ctgggtggcg ttctcggcgc actgctcagc caacgcttac
    33601 cggaacgtat ccgcgtctcc atgacatcta tttttggtct ggcatcgctg gggattggta
    33661 ttttactggt tgtgaaatgc gccaaccttc cagcgatggt tttagctacg ctacttggcg
    33721 ctctaattgg cgaaatttgt ttgctggaga aaggtgtcaa tacagcagtc gccaaagcac
    33781 aaaatctgtt tcgtcactca cgtaagaagc cagcgcatga atcttttatt cagaattatg
    33841 tcgcgattat tgtcctgttt tgcgccagcg gcaccgggat cttcggggcg atgaacgaag
    33901 ggatgaccgg cgatccgagt attttaatcg ccaagtcatt tcttgatttc tttacggcga
    33961 tgatcttcgc ctgctcgctg ggtattgcgg tgtcggtaat tagtatccca ttactgatca
    34021 tccagttaac gctggcgtgg gctgccgcgc tgatattacc gctgaccaca ccgtcgatga
    34081 tggcagactt cagcgccgta ggcggtttat tgctgctggc aaccggatta cgcatctgtg
    34141 gcattaaaat gttcccggtg gtcaacatgc ttccagcact ccttctggca atgccgcttt
    34201 ccgccgcctg gtttgcctga caatgcgtgc aatagcggca aagtgatgat agattgtgca
    34261 gtctgcagta aattgaagaa atttgattga cgagacgagg cgaatcaggt ttaatgcgcc
    34321 ccgttgcccg gatagctcag tcggtagagc aggggattga aaatccccgt gtcggtggtt
    34381 cgattccgcc tccgggcacc aaacgcatca aagtacgctg gttaagggat gagatcctta
    34441 acgggcgttt aatacggggt ccggtcataa cgaaatctcc gtgtaagtgt ggttacacta
    34501 tggtttcagt atcggttcct caaacagagc acctgtcccc tcaggtgctc tgttctcgtt
    34561 tccgctaaaa atgcttacac cattccagcc tgatgtcaag atgtctttat tctgtgtctg
    34621 tcgactccac ttcagaaaca tctgaactct gctcgacttc actgtaacca taagagtgtt
    34681 ttctggcctc atgaatacgt ctcttcgttc cctcgtccgt ggataacgaa agttcagctt
    34741 ccagcgtctg ggctgccacc ttcagtgaaa gatcaacata ctccatcgtg gtggcaacac
    34801 tcctgtgtcc cagaatggat ttcaccatat gcagattgcg ctccggtgcc tccatcagtt
    34861 ttgtcgccag tgtgtgcctg aatctgtggg gggatacatt aaaaccacat tccctgctca
    34921 acctgcggaa aaatgatctg acagactgat gggcccgtct ggcacaatac ttatatttcc
    34981 cgggaccata gtgctcgctg tacagacaga catcaaaaag gtaatcatca tccatcgcac
    35041 cgttggctcg cgcacacgcc aggagatgtt tcagcgaaat ctgcaacggc gtcacaatcg
    35101 gaacgctcca ttcacgcctc gttttactcc cttccagttg cagtgcaatc actgcttcat
    35161 cctcatggac atctttcagt cgaatatgtt tcagttgatt aaaacgcatt cccgtaaaac
    35221 gcaaaacatc cagtactgta agccagtacc aggccgggta catggcatta cgcatcctgt
    35281 cacaaacaac ctgacgctta acctccgctt cagcaaaatt ctgaacggta agataaacct
    35341 taatcatctg cttctcagta agcgtttttt tctttttgga ctccccggga acggaagcat
    35401 catccagtgg attctccgtt tgcggcaacc actttttcct gatacctaac gccagaattg
    35461 gtctgagatg tgacacccta ttattccagg tatttgtcga acgcccctga actttcagca
    35521 tatggcgccg ccatctctga atatctctgg ctgtcacatc tccgggccag gaattccctc
    35581 ccataaactc attaaacaaa cggacaacct tgcggtaact atgctcagtg tccggcttaa
    35641 gatttttaat aaggaaatac tcctcaagta attcctccca gctcattttt tcgtcgctca
    35701 ccatcaggac atctcgctga actcatgacc agcacagtga tttcagggaa atatgataaa
    35761 tgcactgtcc tctggtactt tgttataccc atataaataa cgacccttaa ccagatatcc
    35821 gctgacggtt ttataattcc ccgtcttttc tggtaccgaa tacaaacgca cgctaaaaaa
    35881 acgttctcct tcacgtattt tgtggataca ggcacgctca aacaacgcct gaatattttt
    35941 gcgttcgcgc tggcttcccg tcaatttccg gtacagaaaa aaaatgtcag gcacactcaa
    36001 aaaaacatat ccggcaatat aatgcactct cgcagaaggc gtgttggctt caatcattcc
    36061 tgatgacaac cccatcttca gccaggcgat aaaatcctct tcatcgtctc cggctgctga
    36121 tatctctcct gtcgatgaaa caggcgcaac cacctcgcca gatggcgagt catcctcccc
    36181 cttttccggc gccctcgcct cctccgcaaa catgctgacc agcatagctg tatcttcatc
    36241 tgtcggtata ctctccattt cacatgctgc atcgtcctct gaaccttcac tgctgtctgg
    36301 tgtcgtcggt ttctcagcct gttgttgccg catctctggt tgttcctgaa cagactctgc
    36361 agcattgccg gacacggtat tcccttcagc cgcagcctct tccccggaca cagccggtgc
    36421 cacggtgctc tccgttttac cggatacaac cacctccccc gtaacgacct ctgctgaagc
    36481 aatatgactg gctgtggcaa caattccacc gtttaacaac ggactttcag cctgacgggc
    36541 cgactcctgc agtacatcac ggatgagtgc catttccgga tgctgatgcc agaacgcacc
    36601 ggccagattc tgaagcgctc ccggcaccgt cgcaagccag gctgcagccc cctcaggtaa
    36661 cagacctccg gcaatcactg ccgccagttg ctgagcttcc gtaccctgtg gcgggagctg
    36721 cctgaaccgg aaacgatacg cctcatcagg aacggatatt cccggctgcc atccccggcc
    36781 gctgaacaat tcgccttcca gatgcgcaag cagaggcagg tgataaaaca gggctgacca
    36841 gaaaatcact gcatgccagg ccgccccctg tgcggcctgc tcttccgcag aggcgcccgg
    36901 cggaaacata tgtgcacggg ccagacggac cgcgtaactg gtatgcaaca acgtcaggtc
    36961 cccgaatccc cctgcatgtg accagcgccc ctcccgggcc gccgggacat tctgcgtgga
    37021 gagaagcagg ctgtatacgg gagcacaaca catcgcctca cacaatggct ttggcatcat
    37081 attgttatcc cgtatctgct tcagacactg cgccctgttc ttcggcatca gcagtgaatc
    37141 tgtcgtcagc ggagcataaa agccttccgg tgttcccggc actgaccgcc cgtcatcggg
    37201 tatctccact gctgaaccgg aggtaaaaaa cttccgtaac ttaaacatcc tttccctcca
    37261 ccgccttctc tgtgccacgc tcatgcgggg acgtcgtctt ctccttcgtc ctgctctttt
    37321 cctccgccag ttgctgcatg agctcattga gtaccagcgt cctgcgtgcc cgggtcaccg
    37381 ccacatacaa caggttagtt tcatcccggc gctcctgttc tgacatcagc ggatcagcga
    37441 tatcctgaaa atcatccccc agtacaaccc gctcccattc cagcccctta ctgcggtgcg
    37501 ccgttgaaac cgtgatatca gcctcccgct cgttcgtgac tgtgtgctcc ctgagtagct
    37561 ccagtttttt tggtaacgga aaacacagct ccagcagtcg tatggactga ttcatttcca
    37621 catccttcgt caccttcgca atatgacggt attcatcaaa attccggtaa tccctgcgca
    37681 accggtcaga gtgcatccgg gccggcatat ccatctggaa ccagtaaaga tcttcaagcg
    37741 cctccgttcg gtatccctca atgcccccga cccagtaaac cttcctgccc gccattgccg
    37801 ccatcagcgc cgtgccaatc actccggcga cggtccggtt cagtaccgcc cggtgtcccc
    37861 caaattttcc atgcttaatg cgctcacgca tttcctgcat ggtaagcacc tcatcatcac
    37921 cgccgcagcc gctcacctcc cgactttccc ctgcgcgttt cagcagcaga ttagccatcg
    37981 cggccactgc ggggccaaaa cgaaaactct gtgtcatcca gagacggtcg gcaccggcca
    38041 gagacggatg agacagggca tcatccgcgc cacgaaaacg gtagatctgc tgataccggt
    38101 caccgaccag cacaatcctg caccgctgtc ccagtacaag agcggaagtc accggattcg
    38161 catcctgcgc ttcatcaaac agaatggtgt cccatctgcg cgagagatca ggtccggaga
    38221 gctgaaacaa tttgagatag gtatcatggg tgaccggaaa cgttccatcc ggatcgctca
    38281 tggcctgcca gagcgtcctc acagcacgaa gtgcctcatc cggagcaacg cctttcattt
    38341 cgtccttgtc cggcagatgc tgcctgccgg gggttgcatc cgctgagccg ataaactgat
    38401 tcagccctgt catggccacc cgggccagtg cccagtatcg ggtattcagt tttcttgcca
    38461 catcggtgac gaacaagccc ggaacaagcc gcttctggta atgccttccg aagcgggcaa
    38521 acgccagctg atgcgacgtt ttgcattcaa cgttaaaagg aaacctgcgc tcagcttcat
    38581 cacgaatcgc acgattatag gcgagataga gcatcctgct ctccgggcag gccatggcat
    38641 agctgaccag tgtgctggtt ttccctgtac cggcaaaagc attaacaaca agcttcctcc
    38701 cctgccagtg aataattgcc gtctgttctt ctgtcggttt atggggcatg gctttcctcc
    38761 gtaaagacga aatttccggt cactctgcct tcagccaggc gaccgtgacc agcattaatt
    38821 taccctggtg accagaaaat tttaccgccc gtaaattttt agcgacctga aaagaattaa
    38881 ttatcgccac ccgggtatcc gctgttcatt atcccctcag acacagccca caacgggcct
    38941 gctgtatcac cgttcttcgt aacggtcatg ctctttaaca atccgggagc ggccacgccc
    39001 tgaagccggg ctgaggagga aaacagatat gctacgtaga gagtgacgtg ccgcgcctgc
    39061 agccacaggt aacactccca acgccggaca ccgcaaataa tggctacaca acccgaaaac
    39121 gcactgagcg ccagaacagt acgtttccgg ttgtgccgga gcctgcaccc tgcagagtcc
    39181 tgaacaacag ccagtaaaac caggcacttt cggcgacatc attttcccca tggtgatgtc
    39241 gccttttttc agcacagaat gataatgcac agcaggagta cagataatgg catcagcgca
    39301 ggccaccccg ttcccgtata aatactgtat tcaggccacc atcgaaaaac ccggcggtac
    39361 accggtttca tggattcgct tccagaatac gcccatgacg aaacaggagt gtgaaaaact
    39421 cttctccact gaaaaagaag ccggtaaatc attcaccgtt aaagtcgggg ttactcaatt
    39481 ttcatgtaag gaaaatccac ctgcccctgg agagtgattt ttattatgcc tcatcccatc
    39541 acatatacag attttcttcc ggtggttctg tttttcgtta tcgttatttt tattatcact
    39601 tttataatag ccaccgccac gaccatcccc gcagatatga ctgtaagggg cttttacaac
    39661 agatacagaa aggatttttt gtctcttttt tcagcatatt tcctttcatc aatattctgg
    39721 gcattcctgg tgacaggcac cagtatttca gacagcacgc ctgttttcgg taatacaccg
    39781 gacaatgcgc ttacaaactc gctgatttcc ctggcgttct gtaattcgtt attaactcac
    39841 gcatggattt gttatctctg caacatacac agagacaaaa acacctttat ttatataacc
    39901 ctggcttttc ttcatcttat tttaatcaat tcatgtgccc attttattta cgccacgttt
    39961 tattaattca gtcaactccg tcttaacaga cagaaatagt gaaaaaggtg atactgatga
    40021 atatattaag atacttaagt atttcgatct tcgcgttctt attttcatat accgccacag
    40081 cctccactca gacatcatca tcgaacaacc acgccgaaaa acgtgcaatc ggaacgctga
    40141 aatttatacg ggccgacgga caccgaccgt ttggtcaggc gatgcaacaa ttattaccca
    40201 caggctggcg tactgagatt gcccgtgacg tccggctccc cgccagaata tcatggtccg
    40261 gaaatgacca gtggaaccat gttatcgaaa aaacgctgcc ccgctacagc ctgaaagcca
    40321 catttgatac ctcaggaaaa cgcgtactga tccagcaggc ggaaaaaacg gtaaaatctg
    40381 gcctgttctc agcccccgtc accggtgtca aaaaaacaga aacacgtcat attgagcagg
    40441 gaatgaccct gcgtacaggg ctggaaaaat gggcagccgc cgaaccctgc gctaccggaa
    40501 aatggaaagt ggcatggaaa acgccggtaa attacagtat cgatgcccgg ttagtttttt
    40561 caggtaattt tatggatgca ctgaacagta cattcgcact ttatcagcag gctgaaaaac
    40621 ccttatatgc ctcggtgaat aaaaaccagt gtgtgctgat aattactgac atcccgggag
    40681 acagctaaat gggatggctc atgcccctca taatcgcact gtttatcatc gggaactggt
    40741 ttcatgccgg caatacggag cgggcaataa aaaatactga tgagcagcaa agacaacaac
    40801 aggtatatga aatattacgc ctgacaaatg ccattaatga ctggaaatat catcatcccg
    40861 gtgatatccg tgaaggaaag atgcttacgg ttaacgaaac cggattcgct ccggtatacg
    40921 gaacaaaaca tattatttca gggcaaagaa cttttgtctg gcttcctttt cagccagggc
    40981 tgatatcagc cctgaataaa gtgagccgaa actcagcatt aattgcgcag gtcagggaca
    41041 gaaagttact ggataaccag ggaaataata ttggtgttcc gccttccgtt ataccggaaa
    41101 atgcgatcgt cctgatcaat taagcatccc caaaaggctg agggctcccc ataaatcagc
    41161 gctaatcagc ccaaaagggg attctgtact gaggtctgca tatcatgtct tctctcagcg
    41221 acatgatata tcgctacggc gcagacttca gtccggaaaa tctttctcag tgttcattaa
    41281 cacatgaaat acccgtcgct ctgtctcact gaattgttat ttcgtccctc cacagacaga
    41341 gaataaatat tcaacaagca ggtatacaga atatgaaaaa atttttctgc gctgtcaccg
    41401 ccttatccgt tgttttatca ggctgcagca gttttgaacg aatcagagcc acagaaaaaa
    41461 atgcatccga tgagataaca cgggctgaac gtatagccgg tgaacttcgc aaacaacgcg
    41521 ccgttgtgcg cgaaacaaac cagcagtgga tcaataccac gccccttccg gaaaaaaccg
    41581 ttacccgggc aataccagat tgcccggtcg tcataaacac ccgggaagcc atcacacttc
    41641 accagattgc acagcggatt tctgaaagct gtcatattcc ggttcaaatc atgtctgatg
    41701 tctggacact gctgggtaca ggagaaaatc gcacacagag gatcagcggc gacattcccc
    41761 cgcctgacac cagtggcatg cagccactga gcaccgtggg gctgtcatca gccgccccct
    41821 ccacggcctc actgccggaa ctcaatgttc aggggctgcc cgctttgctt aacaccgtat
    41881 cttcacgctt tgggatctcc tggcgctatg tgaaaggaaa aattgaattc cactggcttg
    41941 acacccgtac attcccggtc acctacatgg acagccaggt cgcctacaat gccagagtcg
    42001 tcagtggcac catgtcatcc acgggcagtt ccggtggttc atccggcagt tccctgagtg
    42061 gggatgccag caatacgcag acaaccgccg ttgatatgaa atccacactc tatgaagaca
    42121 caaaaaatgc cgtgaattcc atgctgacgc ccggtatcgg aaggatggtg ttgtccaccg
    42181 gctttctgac cgtaaccgac accccacagg ttctggatac tgttaagaat tttatggaag
    42241 aacgtaaccg ggaaatgcgc cgtcaggttg tgctgaatat tgaaattctg agcatcaaaa
    42301 aaaccagcaa agagcaggcc ggaatcgact ggaacgccgt tttcagtgac ggacatctcg
    42361 gtatcgacct gaccagtgca tttactaatg ccgccgatga agtcgtgacc agtggggtat
    42421 cgattattga cggtaagctc accggttcaa aagcctttct gaaagcgctt tcatcacagg
    42481 gtaaagtcag tgtggtcacc caaaactcgg ctgtcaccaa aaacctgacg ccggttccta
    42541 tgcaggtcgc cagccagcag gggtacatag aaagtgtcac cacggatacg acggccaatg
    42601 tgggctcctc cacctcactg aatgcggcaa caatcaccac cggcttcaac atgacactgt
    42661 taccgtttat ccagcccggc tcacaccggt tgcagttact gttcagcatg agcctgtccg
    42721 acaaacctgt ttttgaagtg tttgaaagcg gtggcagtcg ggcccagaca ccgagtgttg
    42781 agctgaaaac catcaaccag accatcgacc tgaaatcaga tcagacggtg atcctgagcg
    42841 gattccagca gttcagccgg aaatcgctca gacagggcgt gggcaccccg tcattcttcg
    42901 gactcggtgg tggcgtggac agtgcggatg acgacaccat tctggttgtg ctgatcacac
    42961 cgcacgtaat ctgacggagg ttcccttgaa cgggtcaatg cagaaaaaaa ttcgtccggt
    43021 ttcactgata tcgggtaaac aggcattcct tgcgggcctt gactggaaga acatacctcc
    43081 cggttaccgg aatgcccggc ggtttgcccg gtcacagggc gcagaactgt atctttcctg
    43141 ccacaaccac gacggtgaaa acggtgatga aatgatgatt gccctgctca gtcgtcagga
    43201 tgcagatatc ccccgtaaca ttcgccagtg cctgtcactg gcaatgttaa tacgcccgct
    43261 actcaaatca ggtgggtatg ccattttcat catcgggaac gacgagaacg acgacgctga
    43321 actcacctgt gcgtttgtat cggtgattaa tggcattctg gttaacgacg ttaccggtac
    43381 cccgactgag atcacggaag cccgcaacac gttcctgatg ctcaatgcgg aaccggaaaa
    43441 cggctgggta cgctacgaac ctgacggttt ttcaccagac agcaggtgta tcccgctacc
    43501 gctgtcaaac ctgatcagca ccagaaagca tccggcagaa gcacgtctgc tccccgtatc
    43561 cagaggccca cagttaatga cagccttact catcatcacg ctgcttggcg catcgtggta
    43621 cggctggcaa cgttatgaaa tctggcaggc ggaaaaagcg gaacgggaag aaagtgcccg
    43681 gaaagcagct gaagcacgta taacgccacc atggacgggg cagcctgaga ccggagaatt
    43741 tatccggcaa tgcagcacaa catggaacca gacgccactg tctgctgccg gatggcgtta
    43801 taccctggct gaatgttcta ctgacggtaa cagcggtaat ctgcgggctt catacaccaa
    43861 tacagcaggc acgacagtga cagccttcat ccggcgcatc actgaactca cagacacccg
    43921 gccgtttatt gtgatgcctg aagggaactc tggcggtgtt gcactgccgg tcacattccg
    43981 gctcccggat aatcctgtcc cggttgattc ccttccggaa accagtgacc tgcaggaacg
    44041 actgaccaca ctcgcacagt cagttcaact gcagattgac tggcaggaag tcaataacag
    44101 cttcactgat gaaaacggaa acatcatcca gccaccgtgg aaagagtatg acctgcagat
    44161 acagacgcta ctcccggcta accagctggc agagcgcttc agcgagccat ctgtccgttt
    44221 tctctccgtc acaagacaac tggaaaatgg tcgtttccgt tatcagttca caggtaaata
    44281 ttatgcacga taaaacaaaa gcgctgctga ttgcagccct gctgttaccg gcaatggcag
    44341 tggccggcaa cgaaaatgac gcgtcacaga acaaacaact gcagattgag cagattcagg
    44401 aagaaaccgc cctgtatgaa gcgatgctgg caagagaaaa aattcgtcat gaattgcgga
    44461 cactggaaca gggtatctcc ccggtttccg gttctgtcac gggacatcag ccggaaaatg
    44521 cggctggcac ctcctccggt tcacaggcat tatcatcccc ccgtctgaaa gaaatctttg
    44581 gtattcaccc acagctttca gcacgcgtca tactccccgg aggaactgaa actgatgtca
    44641 gggccggaca ggcattaccc ggtacactgt tcatggtgga acaaatcaca ccggacagtg
    44701 tgattctgga atacaacggc aacaggcaga cgctacgccc gtgaggtgcc atcatgccag
    44761 agactatcgt actgcctcct gaagatattc gtgatttcgt cgcaattcag catgacgctg
    44821 acggaaaaat cagggtgaaa gtcgtcgagg cgaagaagca catcacctct gtacaacgtt
    44881 acctgacgct cttacgctcc cgctatccgg gaaaaatcag caaattcgat tttaccactc
    44941 ttgacgttat cgcagccctg aacagcaata atgcggaacg tgcaccgggc gttttatccg
    45001 acggagggat caacacagta caaagaaaag ttcttgatta catcagaaaa gccgctcaac
    45061 tggatgcctc agatctgcat atcacaccag gacgcgacaa cacggacttc acctatgtgg
    45121 aagcccgggt tcacggcgaa ctggaagtgc tcgatatcct ccggaaagaa gaaggcctcg
    45181 aattactggg ggccacctat tccggcatga ccgacgtcat caagggcaca cagtttgatc
    45241 ccggggtgcc tcaggatgcg cgtctttcag aacagtacct gaaactggcc ggtctgtttg
    45301 gtgcacgcta ttcccactac ccctgcgtgg gtggtctcta tgccgtactc cgcctgatca
    45361 aggatgacag ccagcaaatc ccgaccttca gtatgctggg ctatcaccct gaccaggagc
    45421 gcactcttcg ccgcattctg cagcgccctg aaggtattat cacgctatca ggccccacag
    45481 gctcaggaaa atccaccacg ctgagaaccg cctctgcggc gtaccttgaa cagtacggct
    45541 tcaacaacac cggcggtatt cttcttcccc gtcgcaggct gttcaccatt gaatccccgc
    45601 cggaagggcg cattcccggt gccattcaga ccgccgtcat ggataccgcg cagggctggg
    45661 tggactccat caaatcagcg ttgcgtcttg atcctgacgg gatcctgaac ggtgaaatcc
    45721 gcgaccacga ttccgctatt acggcgataa aagccgccat gaccgggcat ctgatgctga
    45781 ccaccatcca tgccaacgac ccgattaata tcctcgaacg ccttgagatg gaaggcgttc
    45841 agaccaggat gatcgccgac ccacagttgt tcatcggcct gctgagccag cgtctggttc
    45901 agctgatatg tccgcactgc cgtcgcccct ggcatgaggt ggccaccgga cgcacggatg
    45961 atgaacggcg cctggtggaa ggcttctgta acccggacca ggtgtatctg cgaaatcatg
    46021 aaggctgctc acactgctgg cgtggcgtga cggggcgaac ggttatcgca gaagtcatct
    46081 ccccggatgc gcgttttttc cagttgtacc gggaaaaggg tcgtattgag gctaaaacct
    46141 actggcacag agaactgggc ggaatgaccc gaaatcagca tctcctggga aaaatcaaca
    46201 gcggacaggt tgatccgctg gcagcccatt acatctctcc catcgacgag gacagctata
    46261 cgctgctgca ctgacatgag atttctggaa aaactcaaca acaccgacat tgagctgaca
    46321 gcactgaccg tctggatagc cagaaaaacc tttgccctga accgggtgga gttctacgaa
    46381 tccctgggcg gcatgatacg tgacgggacc ccgtcactgc gtgcactcga atttctctgc
    46441 aatgtcgaaa ctgacttcgg aacccggaaa gggacatcag gcatgtattt tctggcgaag
    46501 gaatgcatcg cgtccatcaa atccagcggc atgttatccg gtgctctgcc cacctgggtg
    46561 ccaccggagg aagccacgtt aatcagaaac ggtgaagagc gcgggaatat tgccgacgcc
    46621 cttttccagg tggccagaac cgttaaagcc cggcgggaaa tgacatccag cctcattgcc
    46681 gtctgcctct atccccttct gctgctttca ctctgtgtgg tgaacatgta caacgcccat
    46741 acccgtatca ttcccatcgt cagtgcgttt gccacggaag ataaatggtc attccagatg
    46801 aaagtgctgg ccggcatgtc ggaatttatc atcgggtacg gactctggct ctgcggggcc
    46861 attatcgccc tgacggttct gatccgtttt tccttcagca ggtacaccgg ccccggccgg
    46921 aggatgctgg acagggtacc gccctggtca ctgtatcaga ccttacacgg agtgaattat
    46981 ctgttcagta tttcatccat gctgcagatt aatattccgc tttcgcaggc actgctcaga
    47041 atggaaaaag gggcagaaaa gaataaatgg ttaaaggaga gaattcaggc aatacgaaaa
    47101 catgtgttat ccggtcagaa tctggccacg gcaatgcgaa attccggtta cgattttccg
    47161 tccagagtat gcatcaataa actgctcctg aattccgaaa gggaggacag tgtggcagcc
    47221 atggcggtct atgccgacca gtggctggaa gaggcaaaaa aacgggtaaa acgcagcgga
    47281 ttaattatta ccggtatatc aggaggtttt gtcttctttt ttattcttaa catgatatct
    47341 gccatctaca gtatcagcga catgctgaag caataatttc cccatacaga atatttctga
    47401 aacatcccat aaaccaggag tgatatatga acttcagagc acgtcttttt tattattttt
    47461 ctttaaaaaa agagaaagag gaggataaag gtttaacctt actggaaatt gccggcgcac
    47521 tgattattat cggtattgtg gttggtctcg caatgagtca gatgtccagt accatgtcat
    47581 ccagtgataa ccagcgcgaa ctgaaaaacc tgcagaccat tgccaccaaa atgaagcagt
    47641 ataaattcca gggccggtat acgggctcag attatatcaa aaccctgacg gaaagcggtt
    47701 ccctgccggc cgatatgatc gccggtggta acaaggccaa aaatgcctgg ggcggtgatg
    47761 tcaccattaa ggcaacaaca gataagtatg gttatacgat taccagcaat aatgtaccga
    47821 aagaaaattg cgtggaactc attaacagcc tgcgcagttc atccatgttt accaaaatca
    47881 tgaataccac cccggctact gtggatcctg taacagtatg tagtaaggac aaaaataaca
    47941 tcacccttga aacaaacagc taatgtttcc ggacagcatc cttaaataca agaggatgct
    48001 gtcactgccg ggattatcat aatgaaatgg ttatatttct ctgtcctgat tttattcagt
    48061 catacatcaa tcgcatctgt gatgaataat aatcatctcg attcctgttt tattcaggcc
    48121 ggaaaacgtt acctgattgc ccctgagctt ctgaaaacca ttgcacagca ggaaagcggc
    48181 ctggtcgcca ctgcgataaa tcacaataaa aataaatccg gaaaaatcat cagtacggat
    48241 tacggaataa tgcagattaa ttcatcgcat attcctgaac tgaaaaaact gggcgtcatt
    48301 aaagacaagg atgaactgct taaaaatccc tgcctgaata ttcataccgg cgcctggata
    48361 ctggcccacc attttcaacg ctgcggcgtt aactgggaat gccttggcag ttacaacgca
    48421 ggattcagca aaaataacac agcccggcgc atgatatatg cccgaataat ccacaaccgg
    48481 tatttcagaa aagattaagc tgaggtgact gtctgtgtct gaattactcc cgatatgcat
    48541 tatcctgatt gtccttgcgg caattctgta tcttgcatac agcgccgcca cggtatattt
    48601 gtcgcaggta cacaataccc gcccacacaa cgctgcggtg tgcatctgtt gtcttctggc
    48661 tgtactgagc atttgcctca acggtgcata tggtgttgac gcgacaggac tgttattcct
    48721 ttccattctg acgggattat taactgtaat gacgctgacg gatattgctg tctgtcggct
    48781 tccccggata ttcacgcttt ctcttattat tctgggcgca gcgttcagat acagccagga
    48841 ctcactggct catgccctgc tcaacgcgtc actgtggttc ggcatgatat acctgttacg
    48901 acgcattttt ctggcagtga aagggacgga agcgctcggc cttggtgatg tgttcctgat
    48961 tgcggggatc gcaatgtgga cacaagcaca acacactcca ctgataatca ccgccgcagc
    49021 ctccggggca tttctgttta tcctcatttt ctgccgtcgc agacgccagc aggcactgcc
    49081 atttgctccg tttctctgcg cgtccctgta tgtgttgacc ctcttccctg attccgttat
    49141 ccacacatcg gagatattca cgtgaaaaaa ttcagactcg atgacgataa aggttttatt
    49201 ctgcttgaaa ttcttgctgg cctgatacta attggtatcg ccacgccaat gatttattca
    49261 gaaattgaag actggctgaa tgaacaactt tatcaaagtg cggctttaca tgctgatgaa
    49321 tataataatg caataaaaaa ttatgtggct gataaaacca gctcactttc cagtaaaacc
    49381 atcacggtaa atgaattaat tcagcaggga tatctgaaca acggattttc ccgctccccc
    49441 tttggacaca gctatattac cggtatcaga aaaaataatt tatccggtcg tctggaagca
    49501 cttacctgca caaccggagg acaggatatt aaagaagatg ggttacgtcg catagcgggc
    49561 cagattaatg gactcggtgg ttttatgctc cagaataatt ccgttacagg tgcctttggc
    49621 ggctggaccg atctgggaag taattatcag attacctgca acaagggcca tatcgccatg
    49681 cgaatggctg gaaaagatct ggaggaaagc gatcggcttt accgctacag cgttccggga
    49741 agacccgatt taaacagaat gcatactggc attgatatga ataataacag catcaccaat
    49801 atcaatgaag ccagtggtaa aaacgccaga ttttcaggcg atgtcacatc aaatcgttgg
    49861 ttacatccaa atggtggcgg cttcaccatg acggacagcc agtggatacg tgctgttaac
    49921 aacaaaggga tcacaaccga aggagagatc aaaggtggaa ctgtccgtgg ccagacagtc
    49981 actggaggaa cggtccgctc tgatggtcgt cttactgccg gcgagtattt acagctaaac
    50041 ggaatcgcca acgcaggcac tcattgctca ccagacgggc ttgtaggccg cgacaacagc
    50101 ggggccatac ttcagtgcca atccggtatc tggacaacga caaaaatgaa cttcactacc
    50161 agtacataca atatcgggaa aaatactcgt aatctgagca ttggcgtaca tgcgtactgc
    50221 tcgtggacat accttaatgg ctcgcctttt ggtggttttc agcagatata cgccgacaaa
    50281 aacaaggttt ggtatgtgaa taattatgcg tggggaaatt atgagtccgg tggcacaatt
    50341 acagtgacat gcctcaacct tcctggtgcc ggaatctaac caaaagggca acagtaagca
    50401 tcgacgtgct cttcatcaac accatctccg atgccgtaca ttcttgtacc tgtcatgacg
    50461 tacccggcag ggcactgaat cggcttgtaa taaatagaac ctccgcttct gccaccgata
    50521 aaatgtgcgg aggtcgcttc ataccattta caggcagaat agttcacttt attgcctcca
    50581 agccacaaac cggattagca ctggcctccg tgccaaatcc ggtgtgtggc gaacgattgg
    50641 tgcacctgac ggaagttaca gcaatctggg ttctcaccgg ggaacattta atgggaggaa
    50701 taacggcaga ggaactttgt ttgtgtacgc ttccggtgga aatggtgccg gtggcggtga
    50761 ttgcgccaac acatcaaatc ttcaggtcta tgtgaacggc actttcattg gtatgaatgc
    50821 cagcaataac ccctcctacg gaaagacagc atttatttca tttgccgtgc cagtgggagc
    50881 gtcatatcaa atcatctcca gaccaacaca aaattacgcc tgtggtaacg gtgttttctc
    50941 tgtgtatgcc tatcagatgt gagtccctag ctggctttaa caatgtagtt gaatgcaata
    51001 tttttgggac gcatttctgt ggtactcccc tgtgcccagt atggcatacc actatgatgc
    51061 cctttcagcc atccgccacg ttcgtgatag acgcctgatt gatcggcctg ataactcagg
    51121 agttgtctgt tccgatcacc atcaagtccc ttcccgttat cccagccacg gataaaaaca
    51181 ccgcgcagat cgggcaattt acagccagga taagcacggg caagctgagg ataccgggaa
    51241 caggaaaacg actggccgtt catcaacaga tagccttgtg gaggagtggc agatggccac
    51301 gggataggac ttcccacggg gtaggattca aaccccgacc aggcaccgga ttggcacaga
    51361 gacattgcat ttctgattca gtattcctac cttcctggga tttatcgtgg aaggcagata
    51421 tgtttcgaaa aataaaaatt cgcaaaatga ctctcaaccg ggcgctggac aaatacctga
    51481 agacagtatc catccacaaa aaaggtcatc tgcaggagtt ttatcgcgtc aatgtgatta
    51541 aacgtcaccc tatggccgag cgctatatgg atgaaatcac caccgttgat atcgccactt
    51601 accgggatca gcgtttggct caaattaacc cacgaacagg gcgtcaaatc accggaaaca
    51661 ccgtccgtct tgaacttgct cttttatcat cattattcaa tatcgccagt gtggaatggg
    51721 ggacatgccg tatgaatcct gttgaactgg tccgaaaacc caaaatcagt agtggacgcg
    51781 atcgtcgcct gacttctggc gaagaacgcc gactctcccg ctatttcaga gataaaaatc
    51841 agcagctcta cgttatcttc catctggccc tggaaacagc aatgcgacag ggggaaatcc
    51901 tcacattacg ctgggaacac ctcgatttgc agcacggagt ggcgcatctt cctgaaacga
    51961 aaaatggttt accacgcgat gtcccactct cccggaaagc tcgcaactac ctgcaaattc
    52021 tgccacaaca gataaacggc aatgtgttca gttacacctc atccggtttc aaaagcgcct
    52081 ggcgtaccgc acttctggac ctgaaaatag aaaatctgca ttttcacgac cttcgccatg
    52141 aagccatcag ccgttttttt gaactcggca cactcaacgt catggaggtt gcggccattt
    52201 ccggccatcg ctctctcaac atgctgaagc gatatactca tctgcgggcg tatcagctgg
    52261 tcagcaagct cgatgcaaag cgtaaacaga cctgcaaaat cgccccctat tttgttccct
    52321 atcccgcaac agtcgggaac agaaacggac tgttcattgt gacactgcac gatttcgacc
    52381 ttgaaacccg tgctgaaacc cgggaactgg caatatctca tgccagcgta ttgttgctgc
    52441 gcacactggc acaggcggct cagcggggtg aacgcgttcc aacgccaggc gagctacccg
    52501 caaacattga tgcacgggtc atgatttgtc cgttaacaag ctgaaggcat atcagaatga
    52561 aaataaacta taaattccgg attaacttaa tcacagtcat tctgtctcag gtcctgatac
    52621 agatatattt gtggcataat attccggtat ccggacatat tccactcccc attatattac
    52681 ttatatccat attcttatca ggcctggcca tctgtactgc attctggctg gcatatgaca
    52741 gtgacagatt taagaaacta tcactgccaa agcaaattat tctgttagtg ttatatactg
    52801 ccggattcac ttttttaata ttatacgact tcatgtaata aacaacagtt ctggaaacaa
    52861 ccttatgacc ttataaacaa gggcgcctgc aatactcccc acgcaataca aataaccact
    52921 tctgccgcag aatgcagacc aacaacatat ctggccacac ccagccctgg gcccctacaa
    52981 agagtggtta acactctttt aaaacactcg atcataacga tcgaaataac tcatttccgt
    53041 ttaaaaaaca ctctctttag actcaactta tgaacacaac gcgctgagcc gcacggagaa
    53101 cagaatgaaa ccaaacagca ctggcattgc tgcccgcatg atactctccc tggacagggc
    53161 ccggatttgt gaagccctgc tcaacaaccg ccagctgcag cccacgccac tgcaggttcg
    53221 ttatccacag ggcgtcaggg aagccctcag catcatgagc gaacagcttt ccctctccgt
    53281 ctctgacctg acgcgtatcc tggtcgaaga ggcattaagt gagatgtttc tccccgcgga
    53341 taacagcgtc cgccgtcttc acgaccgtat ggaatatctc atgcaggtgc atgagctcag
    53401 cccggtcacc atggccacat tactggcccc ctggaatatc cgccctgccg tcttccggga
    53461 gccggacaga ctcactgact acctaaccgg ggaaatcctt gcctcactgg ctgactggtt
    53521 ttatctcagt cctgaatggc tgaacggcag ggtgcattat ccgctgtgtc atccgggaga
    53581 ctggcccgaa acacaggacg cgttctgccg tcttatttca gaagacgata atatcgatat
    53641 tattctctgg catggttttc ctttttccgg tgaacacgct caggaatatt gtggcgttct
    53701 tctgcgccag aaaaagaaaa ttaacaatgc cattatttac ccggtacttt ctttatatcc
    53761 gacaggaatg aacaaaaaga aagagggctg gtttcagatg gccagaaaaa tgtcaccgga
    53821 tatccccgtc agggccgtca cgctcagtcc ggcacaggcg gaatatctgg tcacaggaaa
    53881 aatattaccg gctgagttat tccgaattcc cttatcgccc tggtaataac acgttattaa
    53941 acactgactg tttttaatgg cacaggcgtg ccacgccttt gctatatccg aaagacctta
    54001 atacaggaaa ctgaaatgat gatgaaaaag catattaccc gtacacttgt cgcgtctgcc
    54061 gttttatttt cctttaattc agcggccgcc acctcatatt ctgaagcccg taatgatgca
    54121 atgggcggaa ccggcgtcgc ctcctctcac tatggcgtgg caccgctggc aaacccggcg
    54181 ctgctgacaa aagccggtcc tgacgatgat ttcagccttc tgcttccgtc cgtcggtgcg
    54241 caactctccg acccggataa tatcaccgac aacgccgacc ggatcagtga tgactggaag
    54301 gccttcgacc gcgccatcga cagtaaccac ggtgttcctg aggctgccgc acgcctgaaa
    54361 gaacgcctcc gggacttccg tcacacacat gccgccgcac agttgggcgt ttctgctgtg
    54421 gcggcccttc ccggtgaccg cctctctgcc gcactgatgg ttaaatcaca cggcaccgtc
    54481 agtgtcgacg gtaaagtctc tgatgctgac ctcacttacc tggaagaggt ggcaaacagc
    54541 accggacagg aggtggacaa aagccgcctg acatcacagg cctttgcccg cgcggcactg
    54601 atcactgatg tgggtattgc tctggctacg gaactggaaa cggccgggca gaaatggtct
    54661 ctgggcttca cccccaaatt ccagcgcgtc gacctgttca actacaacgt gctggtcaga
    54721 aattatgaca gcagcgcctt caaaggcgac cgctaccaca acacgaaaaa tggcatcaac
    54781 gccgacatcg gtgccagtac ggacctggat gacaactgga cgctggggct ggtcgcacag
    54841 aacctgatcc cccgtagtat tgagacaaaa gaagtgaacg gtatcacgga aaccttcagg
    54901 atccgcccgc aggtgacggc cggtgtctcc tggcacaacg cgatgttcac caccgcattt
    54961 gatgtggatc tgaccccggc cagcggtttc acctccgaca gcaaccgtca gtttgccgcc
    55021 attggcgcag aatttaatgc ctggaaatgg gcacagctgc gcgccggtta ccgtcagaat
    55081 ctggccggta acgacggcag tgcattcacg gccggggtgg ggatctcacc gtttgatgtg
    55141 gttcaccttg atgttgcagg cctgatcggc acggacaaca cttacggtgc ggtcgcacag
    55201 ttccagttca ccttctgagt tccacctgca aaacagcatg cacattatcg ctgcatgttg
    55261 tttccatcat caaaagtctg tcggacatcg acagacttaa ctcccccgga taatacagaa
    55321 aacctgtaaa ggattgaccg gaaacaacaa agatcaaatt ttgatcacag agttcaaaac
    55381 acaaacacca agtgatcaat atctgatcaa tcaatcactt gtgtttatct tttttttatt
    55441 ttgttacatc tgtcatacaa atataactga cagtgattat cattataccc ctatcagtta
    55501 cgtgccgtga ctgatagtta cccgttgtaa ttaaatgtta tcccataacc acaactcaga
    55561 aatatcggag ttcacgtatg aataaaattt attcactgaa atatagtcat attacaggtg
    55621 gattagttgc tgtttctgaa ctgacccgga aagttagtgt cggtacatca agaaagaaag
    55681 ttatcctcgg tattatttta tcctcaatat atggaagtta tggcgaaaca gcatttgcag
    55741 caatgctgga tataaataat atatggaccc gcgattatct tgaccttgct caaaacagag
    55801 gagagttcag accgggtgca acaaatgttc aattaatgat gaaagatgga aagatatttc
    55861 attttccaga actacctgta cctgactttt ctgctgtttc caacaaaggt gcaacaacat
    55921 caattggagg tgcgtacagt gttactgcga ctcataacgg tacacagcat catgcaataa
    55981 caacacagtc atgggatcag acagcatata aagcaagtaa cagagtatca tctggcgact
    56041 tttcggttca tcgtctgaat aaattcgtcg tggaaacaac aggggttacg gagagtgccg
    56101 acttctcact ttctcccgaa gatgcgatga aaagatatgg cgtaaactac aacggtaagg
    56161 aacaaataat tggcttcaga gcaggtgccg gaacaacttc aacgatatta aacggcaaac
    56221 aatatctgtt tggacaaaac tataatcccg acttgttaag cgcaagtctt tttaatctgg
    56281 actggaaaaa caagagttac atttatacca acagaacccc ttttaaaaac tcaccaattt
    56341 ttggcgatag tggttctggt tcttatctat atgataaaga acaacaaaaa tgggttttcc
    56401 atggtgttac cagtacagtt ggttttatca gtagtaccaa tatagcctgg acaaactacg
    56461 cgttatttaa taatgaccgg gtaaacaatt taaaaaataa tttcacaaac actatgcagc
    56521 tggatggtaa aaaacaagag ttatcatcga ttataaaaga taaggacctg tctgtctcag
    56581 gaggagggga attaacgctc aagcaggata ccgatcttgg cattggcggg cttatattcg
    56641 ataagaacca gacatataaa gtgtccggaa aagataagtc ttataaaggt gccgggatag
    56701 atattgataa taataccacc gttgaatgga atgttaaggg cgttgccgga gataatctgc
    56761 ataaaatagg tagtggtact ctggatgtaa aaacagcaca gggaaataac cttaaaacag
    56821 gtaatgggac tgtcatcctt agtgctgaaa aagccttcaa taaaatttac atggccggag
    56881 gtaaaggtac ggtaaaaata aatgccaaag acgctttaag cgaaagcggt aatggcgaaa
    56941 tctattttac cagaaatggc ggaacactgg atctaaacgg ctatgaccag tcatttcaga
    57001 aaatcgcagc aacagatgcg ggaacaaccg taacgaactc aaacgtgaag caatcaacat
    57061 tatcacttac taatactgat gcatatatgt accatgggaa tgtatcaggt aatataagca
    57121 taaatcatat tatcaatact acccagaaac ataacaataa taccaatctg atctttgatg
    57181 gctcagtcga tatcaaaaac gatatctctg tccggaatgc acagttaaca ttacaaggac
    57241 atgcgacaga acatgccata tttaaagaag gcagtaacaa ctgtctaatt cctcttttat
    57301 gtcaaaaaga ctattctgct gccataaggg accaggaaag cactgtaaat aaacgttaca
    57361 atacggaata taagtccaac aatcaggtag cctctttttc ccagcccgac tgggaaagtc
    57421 gtaaatttaa tttccggaaa ttaaatttag aaaacgcaac cctgagtata ggccgggatg
    57481 ctaatgtaaa aggacacata gaggctaaaa actctcaaat tgttctggga aataaaactg
    57541 catacattga catgttctca ggaagaaaca ttactggcga aggttttgga ttcagacaac
    57601 aggttcactc cggggattca gcaggcgaaa gtagtttcaa cggcagtctg agtgctcaaa
    57661 acagcaaaat aactgttggt gataaatcaa ctgttactat gactggtgca ttatccttaa
    57721 ttaatacaga cctgattatc aacaaaggag ctactgttac cgcccaggga aaaatgtatg
    57781 tagataaagc tattgaactg gccggaaccc tgacattaac aggcacccct acagaaaata
    57841 ataaatacag cccggcaatc tatatgtcag atggatataa tatgacagaa gatggtgcca
    57901 cgttaaaggc tcaaaactat gcctgggtca atggtaatat aaaatcagac aaaaaagcat
    57961 ctattctgtt tggtgttgac caggataaag aagataacct ggacaaaacc acacacatac
    58021 cgctggctac aggtttgctg ggtggctttg atacttctta taccggaggt attgatgctc
    58081 ctgctgcctc agccagcatg tataacacct tatggagagt aaacggacag tcagccctgc
    58141 aatcattaaa aacccgcgac agtcttttgt tgtttagtaa catagagaat tcgggtttcc
    58201 atactgtgac agtaaacaca ctggatgcca ctaatactgc tgtgattatg cgggctgatc
    58261 tgagccagtc tgtaaatcaa tcggataaac tcattgttaa aaatcagtta accggaagca
    58321 ataacagtct gtcggtcgat atacagaaag tgggaaataa taactcagga ttaaacgttg
    58381 acctgataac agccccaaaa ggaagcaata aagagatatt taaagccagt actcaggcca
    58441 taggtttcag caacatgtct cctgtgatca gcacgaaaga ggatcaggaa cataccacgt
    58501 ggaccctgac cggatataag gtggctgaaa atacagcatc ttccggtgca gcaaaatcgt
    58561 atatgtccgg taattacaaa gccttcctga cagaagtcaa caacctgaat aaacgaatgg
    58621 gggatctgcg tgacaccaat ggcgaggccg gtgcatgggc ccgcatcatg agcggagcag
    58681 gttcagcttc tggtggatac agtgacaact acacccatgt gcagattggt gtggataaaa
    58741 aacatgagct ggatggactt gaccttttca ctggtctgac tatgacgtat accgacagtc
    58801 atgccagcag taatgcattc agtggcaaga cgaagtccgt cggggcaggt ctgtatgctt
    58861 ccgctatatt tgactctggt gcctatatcg acctgattgg taagtatgtt caccatgata
    58921 atgagtactc ggcgaccttt gccggactcg gaacaaaaga ctacagttct cattccttgt
    58981 atgcaggtgc tgaagcaggc taccgctatc atgtaacaga agacgcctgg attgagccgc
    59041 aggcggaact ggtttatggg gccgtatcag gtaaacggtt cgactggcag gatcgcggaa
    59101 tgagcgtgac catgaaagac aaggacttca atccagtgat tggtcgtgcc ggtgtggatg
    59161 tgggtaaatc gttctccggt aaggactgga aagtgactgc ccgtgccggt gtcggttatc
    59221 agtttgacct gctgaataac ggtgaaaccg ttctgcggga cgcctctggt gagaaacgaa
    59281 tcaaaggcga gaaagacgga cgcatgctca tgaatgttgg tctgaatgct gagattcgcg
    59341 ataatctccg ctttggtctg gagtttgaga aatcggcatt tggtaagtac aacgtggata
    59401 acgcggtgaa cgccagcttc cgttactctt tctgataaaa acaagggggc cgctttatgg
    59461 ccccctctta tattcaataa aacaaatata aaatagcgtg ctccggagaa caccgtgttt
    59521 tacttgcaga aactctgtat cgttccggcg gactcatatt gacgcgtttt tcgggcgcgt
    59581 catcggcggc gcatacgccc ccccatggaa cgtagtactc accagtgaat aagcgtcaat
    59641 acggcgctcc gttaacactt acgaggagca agcttgcggc aacggctttc caggtgacag
    59701 tagcacggag ctttcccccc ggagaacgcc ggcaagcccc ataactgact tcgcttatgt
    59761 tgtaaacacg tcaaagggtg attatctgaa ccttattatt gaaaccaaaa atgttgatgg
    59821 caaacgcgaa ctcagacatg aagagcatga taaaataaaa cacgcacaga aactcttcga
    59881 acaaatcagt aaatccataa agataacttt catgacccag ttttcaggtg ataaaattca
    59941 cgacctgata aaaaaactgg cccagtaaac acggagagtt atttacaata acaactctgc
    60001 catcactgac ttattaaaaa agcgccacaa aatgtggcgc attgtttcat tacactattt
    60061 ttactactgg ccccggggac ccgactgatg ctgatgccat ggcgcgttcg cctggctgtg
    60121 tgtccctgat cagacagagt tgtggctgct catgctccgc gacctcccat gcctgactgg
    60181 ctacagttgt tgagccggca ccgtgactga ccagccctgg ataaggcgga tatgtctccg
    60241 ttcttaccgg tttcagtatc cccgcacagc ctgcaagaaa cagcagtatg ctctgctgga
    60301 tcgcatgacg gtaccgggat gcatcattcc cttcaatcca ctgaatcagt acgtcaagac
    60361 agactgactt accggtgatt tcaaaacgat ttctgcacca ttcagccata atcaatgctc
    60421 ctctggtaaa tgaagagggc actgtcccgt gaggaacaat gccctaacgg ggtgaatggc
    60481 ggtcgggtta caccctccgc cagcggttca gattaagcaa ccggttatca ggaataacga
    60541 cttgaggtag cctgaaaaaa gaaactgcaa tccagccagc ccataaactg cgacacgtct
    60601 tacagaagat ggatgtgtct ttaccatcaa tattctcctg cttcaacaaa tgaagcaggg
    60661 aatatcgtca gaaagcaaca ggtcgtgaac aaataactct ttttgtattc tgtaaatttc
    60721 tcagaagaga aaaagaatta attgtcgcca acgctttcaa caggtcgcat catcaacaga
    60781 tgacaacaac cggaaggaca ccgcgatgga gttgactgca gacctgctga aaagccttaa
    60841 caatataaga caaaatgcca ccatatatgt tcacgcatac cactatactg atgatgaaaa
    60901 atgcgttatg gcaccacgct catccctgcc ctctctggag ctaacagttc tgcagtcaga
    60961 aaaaattatc gatctgcttc tgagtggtta cggttatatc aggcatgacg gctatgttca
    61021 gggatatgga gaaaaaactg tggcaacttt cacaatacag gagggtaagc accatcattt
    61081 caggacaaat ctggcactga ataagacggg caatatagcc gataatgcgt tgaatcattg
    61141 gctacatcgt atacgtaaaa cagacatcat tgttctgtct gttgttctta cagaataaat
    61201 tcagttattt actggaaatt tataaggtct ttatagttat ccgccgagaa tacccgtatg
    61261 aaaactaata agaaagacac aaaatggtac attttttacc gtgaaaattc aggcgaggat
    61321 attttgcttg aaatgtcatc atttaaggaa tgtctttcgg catccaaaga attaatgacc
    61381 ccatcaaatt acatgatatg catagaaaga aatggcgaaa gaataaaacg ttgggacagg
    61441 gaaatcatag caggctctaa caaatggatt aactgtccac ctgataactt cgaaatatta
    61501 ggtgagctaa taaccattaa tagaattatc aaaaagtaac tccgacttta gacaatgcat
    61561 taagcaatgc gctataacaa gcaacgggtt cattttttgc gggagaatgc ttgtaagtca
    61621 tcgctgtgcc cgggcatttt cccactatct tttgtaaaat acctttaaca aatcttgatt
    61681 cgtgagctaa caaagatacc catttatcga gttcacattc gttatcaagt gtaaaaacca
    61741 gacctttaaa tttttccact tcagagaacg aattcatggt ttcttcattg cacactctcc
    61801 tctgtatttc cataaaataa atcatattat catcaatcaa catccttgat atcagaaaac
    61861 ctcttatatc tttcttattc acaacatcaa ggtaaggcca gttaagaatc gacttagaaa
    61921 ttttatcatt ttccacttcg ctttcattga actccgggat tgcaacaagt tcaggttctt
    61981 gcgtatttaa gaatgagctt tctggggtat accagtccag agacttaatt ctttcagaat
    62041 aagctcccct caaataaacc atagcattcc acatgtcacg aacaatccct tttgactcca
    62101 tatacaatct ttcaggttca tttttatcag ctaatgacat atatcctgtg cttatgctat
    62161 tttgctcatc aacaacttca tttaccgaat ataatgagtg taaatcactt tcattttttg
    62221 taacggatat atattttgct ttttttctcc ttaaaggact cagttcacaa ccatcgtcaa
    62281 gaaattcaaa aactgacgct tcaacttcaa gagtcgacgc tccagaatca ggagaaaaag
    62341 cgttatcaac actgaaaggt tcatccggat tttttatatg ccgaactgga acattactcc
    62401 aggcttgcct gctcccttgt tcagcttttt catcaaccag gttactgtta tctctgtcat
    62461 aatttatagt atatcctgca ggcaaagaaa cacctgtaac agaaagccca aggaaagtaa
    62521 cattattttc caaccaaaat cctttaactt ttattctggc ctttccttta aaccagggag
    62581 ccactttgag gtatatttta cgatcttcac atacatctaa ttgacgttgt attctgtcaa
    62641 tcactttccg ggtaaaccca tcaagggcaa agaaagaagc aaaaggagca tcatatttat
    62701 tcattctctt tcttaatttt atataaaaga ttttgtctcc ctccacgtat gcctcagaaa
    62761 aaatcctgtt acgcagttca tttcctgaat aagtaagaat aactctcttc aactcatcgg
    62821 aatgtccata gcaacgataa aaaaactcaa gcggatggat gatcacctta tccgcatcat
    62881 ctgttaaaaa ctcaaggagc caatttttat catatggaga tttcagttga taatctgaaa
    62941 aatcataagg taatgacaca ccttctttat atgcctgata aagagaatta atcttccacc
    63001 ctccatttgc ataagagaaa tcaacattaa atattttttc ctccatcaga atttcagcaa
    63061 cacaccttcc tcttgtccag acactgccta ttttaaaaaa agacaaatca gtcaacagac
    63121 attcttgctc ataatatcta tcacaaagag ttccatcggt ctgaacttcc cgaaaggtaa
    63181 ttgctacttt aggttgttca cctcgtcttt tattcttttt taagaaacca taccaccaaa
    63241 caacccagga tgtatcagaa ggaagattta atgaaggaaa atgctctgtc ctgttgattt
    63301 ttcttattga tactttttcc atacatctgt ccctctaaac aacagaactc ttatctcaca
    63361 agcaaatatt gaagataaaa tagttattca cttaaaccag aaacgctctg atgcgaacta
    63421 catccagtat atattaataa tcttcttgcc ttatgctggt gaagcatgat tacacatcaa
    63481 atcatattat cacttatcac aggaagccca ttaatagcat cattccactg aacctttcta
    63541 ttttcatcgt taatgatttt actcacccat acctctgtgc acccccagcg cgcagccaac
    63601 atcttgttgc tccagccttt ttcttttatt gtctttttta cattttcagg actaagtcct
    63661 ccattaatcc atttttcaag atgatgttta tgaataacaa agtcaaactc aggatatgct
    63721 ctctctaaat cctccagtga agttttcttc cacagtggat tcctgtttcc ctcaaaagag
    63781 gcgatatgaa agtgatcatt cagaaacaat gattcattat ggttatagta ttcaaaccat
    63841 attgaattag catttatgta atcccataat tgcgaatcaa tttctttatc tactcgttgt
    63901 gatgttttta atatatccag aaattctttt tgctgcgtgt atgacaatac cccctccctg
    63961 aagagagcat taaccgcagc ctctctgacc tgctcaattg catttgttac tgacgtacga
    64021 taatagttca ataattgagc gcatgagaaa acaataaatc catgtttaat ctgaaccctt
    64081 aaataacagt acccattccg gccatgaaaa ccagaaaact ccaggggcaa aaaagtctta
    64141 tcaaaaccat ccattacatt aaacacaacc ttcattgaaa taaaccccag tttatttttt
    64201 tattggcact tgcaatcctt gagtttaaaa aacaagagta acctgtcaac accacttaca
    64261 taaccataaa aaaaataaca aaaattgacc cacagatgta ataacgccag tcctgagact
    64321 ggcgtagtga taaaaatgat caggccgctg cctgttcctg aacatggcgc agacgatatg
    64381 accagtttcc gaggtaatgc ccgggtgtca gcagaatccg gcgccgctca ttacacagcg
    64441 agttaccaat caccacttct ccggccacac cacacagcga caattgtata tacgccatac
    64501 cagctgccag cgggtcaatg tccgttgccg acacccacat cctgcggtat gccggatacc
    64561 ccgaacggtt cagggtgtca gccatagcca gaatcatact gccggcacca caggcaggtt
    64621 cactgagcgt gataaagggt ttgttctcaa acaacgcttt cacattcccc agctgcatct
    64681 gtgccatcat gcaggccaca ccccatggcg tgaaaaactg gctccggtat ttatccccca
    64741 gctccagctg catgaacacg ctgcccagaa agtcgcagaa tccctgctcc agtccctcaa
    64801 tgacatgggc aagcaaatgc gccatgttga taacatcctg cttttcataa ccactgacga
    64861 ttcgcaagta cttctgctcc cgtgcttcac tgaactgaag ccggttctcc agggcaatca
    64921 cactgcagct gacaaagtcc tcaaacacct tatgacgttt gtgataacgg gcagtctgat
    64981 taaacagcaa aataaaagct ttctgatggt cgataaaggt ggtcatgtgc ttctccataa
    65041 aaaaacaggg aacacatggc cccggggagg aacaagtgtt ccccttcggg ctgaaatgaa
    65101 agatgacagg aaaaaacggg tgccgcgaat gtggcaccct gaggttagtt actggcctga
    65161 agccagcgga accggtaaag gcgcggcttc tgtctgttct tcagcgcgct cctccaccag
    65221 atacagccgg ggctgtggaa gtttttccct ccccggccat tcggcctgcg ggtcactgtc
    65281 cgtatgttca ccaccgtcat aacagttgta accctgtatg ccccggacag gttcgctgta
    65341 ccagtgccgt atctcacagt cgaacacctt cgagatttca ccaaccagct cacctgaagg
    65401 cggataccag ggcgtgtctg tcagcagcac cagactgctc atctcatcac gcacccagcg
    65461 gacattgtga cctgatggcc actccacacc gtatatccgg ctgtaaaggc tggtcgtggt
    65521 ggaaatgccc tgcagcagtc cacctgaacc gttcagctct gtggccagac gggggggtat
    65581 gaccatcagc atgtcacagg gctgcgcctg ttccgggtaa tccgacagcc ggctccagca
    65641 ggcttcggca tcaatgtgtt cactcagtcc gaccagacca aaccagtcag aatactgaca
    65701 atgcattaac gtggtgatta tctcccgggc attatccggc agattccccc agcggactgc
    65761 gccaatacat gactggtgat aaaggcggtc aatggtcttt atcgtctctt catccagtac
    65821 ggcatctttt accagcagcc ccagccactg ttcaaatgcc tggttagccg gagatgacat
    65881 ccccgtacca tgacgaacca gcgccggata tggcggatac tgcgtcgtcc gggtaggttt
    65941 caccagtccg gcacaaccga tcagaaacag ctgtatactc tgctgtatgg catggcggta
    66001 acgcggtgtc tcacatcctt caatccactg cgtcagcaca tccaggcaga ctgacttacc
    66061 ggtaatttca aaacgatttc tgcaccattc ggccatattc aatgctcctc tgataaatga
    66121 agagggcatt gtcccgtgag gtacaatgcc ctcacgggaa aagcggagtt gtcacactcc
    66181 gccatcgtca aaagtccgtc cggttataca accttccggt atttactggt ttctgtcccc
    66241 atttcgttga tagctacgtc gatattgccc gggaaacgat ctgttttaat cttccacaaa
    66301 tcgtttttcc agtaacgtgc cactgtcaca tctggcggaa cgttgctctg cccgtacttt
    66361 ttacgaagcc ggtcaatgca ctcctgcaca aattcattac tgaatttgcg ggattcgtgc
    66421 acgaacctca gcccgttttt gccttcaaac gcacgggtaa taaaccgcac ttcagcctct
    66481 gttggtccat ccagccagga cacattaatc atgttgtaaa cgccggaatc gcataccttc
    66541 agcacgttaa atttctgccc cggaaatgcg aattccagaa cgtcactgac acgggcaacc
    66601 acggcatcgt caacctcagt cacagactta acattcatca ttctgctcct ctgtatgaaa
    66661 atcactgagg gaacagcccc gtgaggagcc gggccctcac gggatggcgg tgtgtcacac
    66721 cgcgttactg ataaagtctt tcagccaggt ggtatgcccg aggccatacc gcctgctggc
    66781 attcataagt tcgtctgccg acggatttat gtaaaggttg tagtaccaga gttcttcctt
    66841 taaatccgcc aggttacccc tgccggtcgc aagccactcc tgcaccacgt catgaatttc
    66901 agatgtgacg tcaccgcggt aattcgatgt gatcgtaaca tccgtcagta acccggcaat
    66961 actgcccgcc agcagacgcg gttgagacat gccgttatag aaccatacgc tgcagccgga
    67021 atctccgggt aaaatattca aatcgggtct catgaaattt ctcctgaaaa agcagggacc
    67081 cgtcccgcag ggatgagtcc ctgtgggaat gtaaaaaccg ttaaaatgca tctggtattg
    67141 cagcgtaccg cttcccggta ctgatactgt cagcccagat actgtgctct gtttcatgtc
    67201 ccagagacat aaccctgtcg agtacctcat ggaaaatcgt tcccggtgct ggagcatttt
    67261 ccatctccag gtcatacagc aaccagccca gagcctcacc aacatcccac aaaatcgagt
    67321 aaagtggtga aggctcatcc tcatcctccg gcaccggggt aattccgaga cgttgccagg
    67381 cgcgaagtat cgccagttca ccttccagct gacagagatg tgccagcaaa gcggactgac
    67441 tctgaagatg ccagtaacca gtaataaaac gcgaatacat aaaaccccct gtctgattct
    67501 ttctgatgaa agagcacacc gtccgaagga acgatgccct ccgggcggtg aacaacagag
    67561 ttcagattgt tcacccgcga ttgttaagca ttacgccgct ttttctgccg gcgtatcgtg
    67621 tacggaaaga aggtattccg ttgcctctct ggcctgtcga catgcactga acagcgcttt
    67681 tttgtcagca cgaagaattt tcagccatgc gccaatataa ttctcatgct gcacatttcc
    67741 tgtgacaccc agttgagcgc acagaaaggc actgcccatt tcagcgataa gctcttcgaa
    67801 cgcataacat tcatcgccat agcgcccacc tgaggaaact atcccttccc ggttaagacg
    67861 tttcgcatga ccggtactgt gtaccagctc atgcagtaat gtggaccagt aatcttccgc
    67921 tgaagtgaac tgtgacgaga gcggcaggac gatacgatca aataccgggg tgtaatacgc
    67981 ccggtcctga ggtaaagaat gaatcttcac accgcaggca ttcaccatcg tcagtacacg
    68041 atcagccaca gcattcagcg tgtcgttcgc accgttctca gtgacggttg tcgtcacctc
    68101 cggcagcgac aatccatcac actgttcaac gttaaagaga ggattcgctt tcagcaccgg
    68161 tacatcaacc ataacggggt taccctctgc gtcgaatatc cggttgccgt tctcatcctc
    68221 atcctgctta tgccagcttt tgtatatcac ggccatcgtg gacttctcgc ccttacggac
    68281 ctgcccaccc agcgcctgag cctgacggta cgtcaaccag cagttactgc tgtaaccggc
    68341 ttcttctgcg gcgagccaca acagcaacac attgatccca cggtaagcgc tacgtgtcag
    68401 tgcattaacc ggcatcattg gatcgccgct gtgagtccgt actgagcgcc atggacgttg
    68461 ccagggggct acgccctttt cgatggcctc aatcaccctg tccgtcacca gctgatacag
    68521 gtcacgctcc ggggttttat tcacgggaca tgaagtatgt tttttcgttt tcataagagc
    68581 acgctccata aaaaagatgc atgctccccg tacggtgaac atgcaccata cggttaaatt
    68641 aataacgaaa atcagactgg ccggacttac cacaacgcca gcatcaccag tgaatgacgc
    68701 tcaaaatcaa acgtcttcag cgtatcgctg acccacgcca ccgacgcagg tacacggtac
    68761 agtaagtaaa agaaaaaccg ttcagtctgc gtcggaaaag tcaatacaga ccgccttcag
    68821 gagaccggct atacgttgat ccagacattc agtccagcca tcgttccatc cacggtccca
    68881 tggattgtag acatcccccc atggattgca gacatcgtta cagccattct tccatgcctg
    68941 ccataccgcc atttccatga cagctcccag cctgtggcct ttcgcacgta tatgccacag
    69001 aaacggccgg aaaagtcctg atttcagttg gtcaccaccg ctttcgtagc ccagatgacg
    69061 agcaatactt tcaagatatt caaatgcatc attctgcatt ccatccgcct tagccagcga
    69121 gctgtacgca tcacgaagta tcgtcatacc ttccagttga tactcacacc acgcacggac
    69181 agacgacact ggtgcgatta cccgaagaat ggtgcaggca cggcatacaa caacgtaaag
    69241 gagtattaac agactattca tgtgttcacc tcagatacaa aaaattacgg gaacacacct
    69301 gccctacggg acacatgttc ccgacagggt aataaggtta aaaacaagga cagaaaaata
    69361 attaccacca tgcccagagc aacagtattt ttcgttcaaa atcaaacgaa tcaagcgtgc
    69421 ttctgaccca cgatttcacg atctcaacat ccttccagta atactggtca tactcctgag
    69481 aaccaaaaaa gaatccttct gttgtgggga agaattcccg gcagttctcc ggcgtaaggc
    69541 attcaagatc gctcagtagt gcctccagtt ccgttctgga aaccggacgc tccacggcat
    69601 tttcaaccgg tccacaatgc gtctcaaacc aggcaatcag tgggttaacc ttgcgaaagt
    69661 aacccacttc ccgcgtttcc ggtaccgata ctaccgcgtc ggtaacacga tcacgcccaa
    69721 ggaaaaaaat atcgagtccc ataaacaatc tcctgataaa aacagcggga ccgtccccga
    69781 acgggagcgg tcccggacgg gttaaggaaa actcagattt gtcatcgcgc acgcgtggta
    69841 cggtcagaca gatccgggcg ttgtgtgata aaccttccaa cggtcatgac gaccgttcaa
    69901 caggcttcag cgaagaccga agcgatagcg aaaagaagta agctgcgcgg ccatacggga
    69961 ggcagcagag agcgtgcggc gagcggatat tcgaccggta aagacattaa accgcacgac
    70021 agacagcgga agtgcctcaa accagtaacg atattcacgg ctgtgagagc cttcacggaa
    70081 ctcaaagcag tgtcgcaatg caccgttggt ggaaacaatg caggctctga ctgacagatt
    70141 gccggtggcg ttaataaacc acggagtgca gtacagatgc ctcagttgtg gagcggacca
    70201 gggctgttgt gggtgaacac aacgcttctg attaaacaga cattgtctga gaagatgaaa
    70261 gaaaggcgtt atgcgcatat ctgcacgtaa caaaagtggt ataaacatcg gaacctccga
    70321 atgaaaaaga cggacgttcc gccctgacgg actggaaaat ccgtcaggtg aacacatcaa
    70381 tgcgtcagca cgatgcgctt aagaagacca gagacaactc atagtcacat tgtatacaaa
    70441 gtgccttcat catcacgtaa aaccgtggtg acatctgctc tcaaaggtgt gcagatttgc
    70501 ccgtacatca gggatgtttc ccttcagtac tcacagctcc tttcaggcta cggaggtgta
    70561 aaactgacca cccgaaggcg ctcctcacag gtcatatgga gcattggtgg tcgccggagc
    70621 gacgtgggcc acccgaaggc gttcctcaca ggccacgatg gaacattggt ggtcgccgga
    70681 gcgacaagtt agcgtctaaa atataatcat aaacaacata aaacttcaag ttgttgtcgt
    70741 ggctatggta ataacctaca actgactgac aaggccagct caatttgata ttttttaatt
    70801 cagtaacact ctttatgaac ctgttagaga gatgtgaaaa tgaaatcgaa atacaaaaaa
    70861 ttaaaagatg aactactcag aatagccaaa gcatgcgccc cgacgccaga agatatgttg
    70921 gtatatacgg gaagagccag aagacttgct tcatttctta aagatgccaa tatacagata
    70981 agctcagcaa atagcataaa actacgtcat attgaatgct attttcaaca acgttatcgc
    71041 actggcgtca gttccaaaat actacgagaa gaattagata caataaagca tgttctcacc
    71101 cattgcggaa agagaaacat cgttaaaaat gaacgactga cttatacatc actgaatatt
    71161 gcagatgtcc gaccaattgt tatctgccca tactgtggaa ataagacgaa tcttataaaa
    71221 ggggctctca tgccattttc catttcagcc gcgacagaaa acaaatatta ctggatttgt
    71281 cccccatgca atgcatgggt tggatgccat aaaaactcag gaagaccatt aggtactccg
    71341 gctaaagaaa acctgagaat actcagaaca aaagtacgta aactttttga caattatcag
    71401 caaagagcca acatatcccg taacgaggca aacatatggc tatccagaaa actgaattgt
    71461 cacattcatg aatgtcatat tgggtatttt gatgaagata tgtgtaatcg cgcatcagaa
    71521 atcatcataa ccgaaattaa caaaaacaca tatcctccag attcatttta aatatcaact
    71581 ttcatcagaa ccgttaaaat aaacctgttt tacataacga cctcttccgt caccatggcc
    71641 aagatccata gaaacacgag cgtagatttc cgactctcca tatccatttt ttcgataaaa
    71701 ttttctggct tcctgagaaa aatgataacg catactgtgc ggagatttca ctcctgacaa
    71761 tcccgcttta cgggcatgat aacgaaaaac attgatagcc tgttttatat ttggacggtc
    71821 aataagtttt ccgtcgtgtt catccattat tttttgagca taaagcaaag catgtcttac
    71881 cgcatctctg tcaataattg tggtatttct tggtcttcca cctttcgtac caaaaacgac
    71941 cgaaagagaa tcgcaccctg aattcagttc ccgtagccat gactgaacag acttacatga
    72001 ctgaacggct tcttttgttc ttaaacccag aacataagaa agctgcattg cagctgccac
    72061 accaggattc tttttttcca ctttagcaaa tgctttccag aattcatccg gtgacactgc
    72121 tattttcttt ccatcacggc atgtatcctt taggccgagc gcttcattac tcaataatgg
    72181 gttagaagga tctgcgattt taagtcgtcc atctttttct aaaatggcac gcataaccgc
    72241 ccgttcattc tgaatagtcc ggtgagaaat acccgctgct ttcctgctga gaaggtaacc
    72301 agaaatatgt ctggttttaa tactgctcgt ctggcgaagt ttaattccat tttcacgtaa
    72361 ataattcagg aagtactttg caattctcat tctgtcagct gttgttgcgt aactgccacc
    72421 acactcacgg gcaagtgaga tgaaagactt gcgaaacagt tcaactttag aacgtgacga
    72481 tttccgggcc ataaagtcat tctccctgta aaaatcagat gcagccccct tccgttcaca
    72541 aagtaaaact gcatacgcag cccttccggt aaacagaaaa gttcgttgat attgttagtt
    72601 tttcgtcatc acggacggct ctgtaacgcg gttgttcgcc ttcctggctc gtttgatgtc
    72661 gatgtagcag agcttgcaca tgttgagttc ttacaggagt tcgctacctg tgcaccaggg
    72721 cgattgttga ggaatactcc ccgtcacctt acgatgacgc ctcccgacat cagaacgatt
    72781 tttcattttt attcctttat ttcagtcagt taatacactt acctctcatg tattacgaag
    72841 tttaagaaga cattccggcg tggccgtccg tgtgaaaacg gacgcgccgg aaaggcgtgg
    72901 atacgtcacc gaaagacgtt gtcagagcga caaaacgagc atcaggacgc ttgtcacgag
    72961 cgacgatttt ctgccagacc ttacgtgatg ctgccttcag ggcagatggc cgcggcgtca
    73021 cttggtgttc ccttcggtcg cacgcaagtg acgccgcaca gcttcaaact ccacatgcac
    73081 acgcaagcga ccaaatttca aatggtctta tgccgtgcgc acagtaataa gaagctcatg
    73141 cggaaagtca gaaaatatct taagacgcag attttaaagg cgtttttatg cctgtctgct
    73201 gagaaaaaat aaaagtaccg gaacggaatc cggaatgcct tcatcatcac gtaaaaccgt
    73261 agtgacatct gctctcaaag gtgtgcagat ttgcccatac atcagggatg tttcccttca
    73321 gtactcacag ctcctttcag gctacggagg tgtaaaactg accacccgaa ggcgctcctc
    73381 acaggtcata tggagcattg gtggtcgccg gagcgacatg ggccacccga aggcgttcct
    73441 cacaggccac tatggaacat tggtggtcgc cgaagcgaca gttaatgcag atgcaggtta
    73501 acaaaaagag aaatctgccg tcaaccatat gaacatgttg atttcattat gaatgtgttg
    73561 ttttaatata aattaatcag taacttcata aaaagaatat aagaaacaga taatgcatgt
    73621 atttacatgc atatcagcac aatgataaat tacctccctg aaaacacgat aaaggtataa
    73681 ttatggaagt ttccgttata tttttctggg ttatcactgc tcttattgcc atcttattct
    73741 taaaatcagc aataacctac aaaataaaaa agcgaaataa aacagacata gtgttatata
    73801 tattggcatt tttatcagcc ggatatacat tccagtatat ttacaatgac aatatatttt
    73861 cagcgctaag gagcattggc ctgtttgtta ctctgatagt actgaacata gtatttaatc
    73921 tcttgtttag tctcttatgc attctctcga aaatgatatt aatgtcacaa cacataagga
    73981 agatgcaaaa aaaactaaaa cagatgcctg tatctgattt tatttattat ttaatgaaaa
    74041 aacacaacaa ataccccaca tatgtattac acatgccatc aaacaatata tttgaaatag
    74101 gctacaatat aatgaacacc gatctggtca ttggcgaaaa aatttatttc aggacactaa
    74161 gtaatcgttc tttatatttt accctcacac aagccccatt aatcgccaca ataaaagatg
    74221 atgtgttttg cactcttcat gattactata accacaataa tgaaacgaaa tccactattg
    74281 acaattacat atcaaaaata aaggataatc ataatacccc atggttatta aacaacgaaa
    74341 acgttcatga ataaaaaaca aagcgccaat tcccgggata aaaccaaaga gcataaacat
    74401 atgaaccgga aattctattt caaaaatatc ctgtggggaa tgtgccccat tataatatcc
    74461 atattgcatc ttatatatga cacaccaaca gaagcatcga taaaagttga cttatttctg
    74521 atactcagtg gcctggctct tccttgttca atatacctga caaaaacaat tacactaaaa
    74581 tacacatcca tatcattctg gaaaaaacct tttttctggt ctt
//
LOCUS       DAZFQD010000005        73041 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.005, whole genome shotgun sequence.
ACCESSION   DAZFQD010000005 DAZFQD010000000
VERSION     DAZFQD010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 73041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 73041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..73041
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.005"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..712
                     /locus_tag="ACHKTW_000352"
     CDS             <1..712
                     /locus_tag="ACHKTW_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFO6461441.1"
                     /translation="AKINLLSRLTPLLFVFAPFLAQSNMTVYPMAVSINSQGEGNVRV
                     ISKSNEVQYIKATVFRIDNPSTPQENEVEIKSGDANHLVVMPPKFALPAGSSKTVRFV
                     AMEPEQKEKNYRVKFEAVPSIDDVATDKKDLSMQLTVNLIWGIVVSVPPQQPIAKLEV
                     NSAQKLINAGNQRLKLLTIAYCKNNSKENCKIQTVNKNIFPGQERNLESISGYDKIVV
                     KYNNWITKDNGEFELAVH"
     gene            742..1242
                     /locus_tag="ACHKTW_000353"
     CDS             742..1242
                     /locus_tag="ACHKTW_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000755097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CS1 type fimbrial major subunit"
                     /protein_id="HFO6461442.1"
                     /translation="MKKVFAKSLLVAAMFSVAGSALAVQKDITVTANVDAALDMTQTD
                     NTALPKAVEMQYLLGQGLQSYQLMTKIWSNDVTKDVKMQLVSPAQLVQSLDASKIVPL
                     TVTWGGEEIKADAATTFTATKIFASDALTNGSLAKNLMFAQTTKGVLETGIYRGVVSI
                     YLSQDI"
     gene            1334..4018
                     /locus_tag="ACHKTW_000354"
     CDS             1334..4018
                     /locus_tag="ACHKTW_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcfC E-set like domain-containing protein"
                     /protein_id="HFO6461443.1"
                     /translation="MDKKLLALLILASLSPAEATLTKIPAGFEVIAQGQQEYIEVYFS
                     GKSLGKYYAMVNLDTVTFLDPASLYNKLELDVDDQKIAHIVKEKLSQPLARHGELACG
                     YVRTDSGCGFLNTDTLEIIYNDEESSATLFINPQWNSAFDAKSLYLNPDKNTVNAFIH
                     QQDINVLAQDDYQSLSIQGNGALGITENSYIGAHWNFNGYDADDVSDSNADVSDLYYR
                     YDFLRRYYVQAGRMDNRTLFNAQGGNFTFNFLPLGAIDGMRIGSTLSYLNQAQSQQGT
                     PVMVLLSRNSRVDAYRNEQLLGSFYLNSGSQFIDTSSFPPGSYSVALKVYENNQLTRT
                     ELVPFTKTGGLTDGNAQWFLQAGKTTSQVSDDESSAYQLGVRLPLHPQYELYAGLANA
                     DDVSAFELGNNWTADLGGVGNLAISASVFRNDDGGKGDMQQANWSNPGWPTLGFYRTN
                     SDGDACTTDSRESYNALSCYESISATVSQNFVGWNMMLGYTRTQNNTDDSLRWDKQQS
                     FENNYLRQTTAQSISETVQLSASRAFVMRDWILSTSVGVFHRNDNGGDNDDNGLYLSF
                     SLSDTPTMDSNNNSHSTNVSTDYRYSEQDGDQTSWQLSHTFYNDSFSHKELGVTVGGL
                     NTDTINSAVNGRWDGQYGNVYATVSDSYDRKNHDHLSAFTGTYSSTLAVSRYGVNLGA
                     SGTDDLLGAVLVDVKGFSEQDEESQDLQLEARVAGSRTLQLGQSDSVLFPYPGFQSGF
                     VEVNDSSQGNQQGTTNIINGAGNRELMLLPGKLRYREVSASFNYNYIGRLLLPAAVKK
                     FPIVGLNSAMLLVAEDGGFTLEINGSEKELYLLSGQQFLKCPLSVVKKRASIRYSGDV
                     TCSVVTYSQLPESIQVQAQLKQPKLRGNVQTAQREVAP"
     gene            4015..5103
                     /locus_tag="ACHKTW_000355"
     CDS             4015..5103
                     /locus_tag="ACHKTW_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CfaE/CblD family pilus tip adhesin"
                     /protein_id="HFO6461444.1"
                     /translation="MRNRLIAAILGLFGTLTGVQAAPDVTSEITYDLASGRADYYFWK
                     DEASAGNNGYMWYECSYPDLQQTCTANGNISTVQIYLTEQRSGMRWPVKLKGFKTAIV
                     SSDEAPPGCKGGKGLQTNLKDSNRSSCTEDGQHYYIYDTKFLTLYLEQTEMKNLPIGG
                     VWKGKVKLHSNSPAQDYFANITLNTLDPNHIDVFFPEFAHATPRVQLDLHPTGSVNGS
                     NYAQDLTMLDMCLYDGFNGNAISYEIMLKDEGRPAAGRRDGYFSIYRQGGTTTDEGER
                     IDYRVKMYNPETGGQIDVRNNENMVWNSINLKRVRPVVLPGIRYAVMCVPTPLTLAVD
                     KFSVMDKQAGYYMGKLSVIFTPSLPTIN"
     gene            complement(5191..6489)
                     /gene="exuT"
                     /locus_tag="ACHKTW_000356"
     CDS             complement(5191..6489)
                     /gene="exuT"
                     /locus_tag="ACHKTW_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
                     /protein_id="HFO6461445.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     IARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVSTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQETHNDPAPQH"
     gene            6972..8384
                     /gene="uxaC"
                     /locus_tag="ACHKTW_000357"
     CDS             6972..8384
                     /gene="uxaC"
                     /locus_tag="ACHKTW_000357"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFO6461446.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGEPLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            8399..9886
                     /gene="uxaA"
                     /locus_tag="ACHKTW_000358"
     CDS             8399..9886
                     /gene="uxaA"
                     /locus_tag="ACHKTW_000358"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HFO6461447.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGVHVHAHNTRTNLSDLDQYRYQPDFQDLPAQ
                     AADREVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            9969..10520
                     /gene="ygjV"
                     /locus_tag="ACHKTW_000359"
     CDS             9969..10520
                     /gene="ygjV"
                     /locus_tag="ACHKTW_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HFO6461448.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAVDERG"
     gene            complement(10525..11769)
                     /gene="sstT"
                     /locus_tag="ACHKTW_000360"
     CDS             complement(10525..11769)
                     /gene="sstT"
                     /locus_tag="ACHKTW_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFO6461449.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPIGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(11974..12276)
                     /locus_tag="ACHKTW_000361"
     CDS             complement(11974..12276)
                     /locus_tag="ACHKTW_000361"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6461450.1"
                     /translation="MASKSPKRSLEAGATRMDARRGRILQGRYLAPDPVAARDKLREP
                     RSGDFVRQSLGVQGAAAIGRPLRAPCASDFIWHNTLKERNRSHIPNSFAHMLSNQP"
     gene            complement(12385..13350)
                     /gene="alx"
                     /locus_tag="ACHKTW_000362"
     CDS             complement(12385..13350)
                     /gene="alx"
                     /locus_tag="ACHKTW_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HFO6461451.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGE"
     gene            complement(13633..14619)
                     /gene="ygjR"
                     /locus_tag="ACHKTW_000363"
     CDS             complement(13633..14619)
                     /gene="ygjR"
                     /locus_tag="ACHKTW_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417558.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFO6461452.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNALHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTACLPNFHLLRQALPKVGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPSFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLASEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKL"
     gene            complement(14698..15381)
                     /gene="ygjQ"
                     /locus_tag="ACHKTW_000364"
     CDS             complement(14698..15381)
                     /gene="ygjQ"
                     /locus_tag="ACHKTW_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HFO6461453.1"
                     /translation="MQPRLLLRICFSRRTLKIGCLLLLIAGATIFIADRVMVNASKQL
                     TWSDVNAVPERNVGLLLGARPGNRYFTRRIDTAAALYHAGKVKWLLVSGDNGRKNYDE
                     ASGMQKALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRAIWL
                     AKQYSIDAIGFNAPDLNMKHGFYTQLREKLARVSAVIDAKILHRQPKYLGPSVMIGPF
                     SEHGCPAKE"
     gene            complement(15458..15961)
                     /gene="ygjP"
                     /locus_tag="ACHKTW_000365"
     CDS             complement(15458..15961)
                     /gene="ygjP"
                     /locus_tag="ACHKTW_000365"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HFO6461454.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPINKVMYDNKIHVLKNALGLHTAVSRVQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            16046..17182
                     /gene="rlmG"
                     /locus_tag="ACHKTW_000366"
     CDS             16046..17182
                     /gene="rlmG"
                     /locus_tag="ACHKTW_000366"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417555.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HFO6461455.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     RGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRMNVETNM
                     PEALDRCEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHHARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKTVKLGRRR"
     gene            17373..17456
                     /locus_tag="ACHKTW_000367"
                     /pseudo
     CDS             17373..17456
                     /locus_tag="ACHKTW_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
     gene            17453..17869
                     /gene="higA"
                     /locus_tag="ACHKTW_000368"
     CDS             17453..17869
                     /gene="higA"
                     /locus_tag="ACHKTW_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HFO6461456.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMSGGIAVIRTLMDQYGLTLSDLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(17914..19932)
                     /gene="fadH"
                     /locus_tag="ACHKTW_000369"
     CDS             complement(17914..19932)
                     /gene="fadH"
                     /locus_tag="ACHKTW_000369"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HFO6461457.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFAHCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVRAVRERVGNDFIIIYRLSM
                     LDLVEDGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFTWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADMVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQACLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYHRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIAGFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVFAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(20258..22609)
                     /gene="ygjK"
                     /locus_tag="ACHKTW_000370"
     CDS             complement(20258..22609)
                     /gene="ygjK"
                     /locus_tag="ACHKTW_000370"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFO6461458.1"
                     /translation="MKIKTILTPVTCALLISFSAHAANADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     GKKVDFTLEAYSIPGALVQKLTAKDVQVEMTLRFATPRTSLLETKITSNKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPVQTEINGNRFTSKAHINGSTTLYTTYSHLLTAQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWRSPGGAVKFNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDSVRPQDVGFVPDL
                     IAWNLSPERGGDGGNWNERNTKPSLAAWSVMEVYNVTQDKAWLAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTESGEMLFTVKKGNKEETQSGLNNYARVVEKGQYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVANGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPTTQ
                     FYYDVRIEDKPLANGCAGKPIVERGKGPEGWSPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGAQQGAPNFSWSAAHLYMLYNDFFRKQ"
     gene            complement(22626..23696)
                     /gene="ygjJ"
                     /locus_tag="ACHKTW_000371"
     CDS             complement(22626..23696)
                     /gene="ygjJ"
                     /locus_tag="ACHKTW_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgjJ"
                     /protein_id="HFO6461459.1"
                     /translation="MKLITAPCRALLALPFCYAFSAAGEEARPAEHDDTKTPAITSTS
                     SPSFRFYGELGVGGYMDLEGENKHKYSDGTYIEGGLEMKYGSWFGLIYGEGWTVQADH
                     DGNAWVPDHSWGGFEGGINRFYGGYRTNDGTEIMLSLRQDSSLDDLQWWGDFTPDLGY
                     VIPNTRDIMTALKVQNLSGNFRYSVTATPAGHHDESKAWLHFGKYDRYDDKYTYPAMM
                     NGYIQYDLAEGITWMNGLEITDGTGQLYLTGLLTPNFAARAWHHTGRADGLDVPGSES
                     GMMVSAMYEALKGVYLSTAYTYAKHRPDHADDETTSFMQFGIWYEYGGGRFATAFDSR
                     FYMKNASHDPSDQIFLMQYFYW"
     gene            complement(23830..25263)
                     /gene="ygjI"
                     /locus_tag="ACHKTW_000372"
     CDS             complement(23830..25263)
                     /gene="ygjI"
                     /locus_tag="ACHKTW_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFO6461460.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTIISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFA
                     WWKYSKYIKGLTAEERHIEATPASNVD"
     gene            complement(25326..25775)
                     /gene="ebgC"
                     /locus_tag="ACHKTW_000373"
     CDS             complement(25326..25775)
                     /gene="ebgC"
                     /locus_tag="ACHKTW_000373"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HFO6461461.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVETDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            complement(25772..28864)
                     /gene="ebgA"
                     /locus_tag="ACHKTW_000374"
     CDS             complement(25772..28864)
                     /gene="ebgA"
                     /locus_tag="ACHKTW_000374"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HFO6461462.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SGQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLVGKHLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLAASPVVTTLEYTLFDGERVVHSSAIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDANGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDNGNVWYKFGGD
                     YGDYPNNYNFCLDGLIYSDQTPGPGLKEYKQVIAPVKIHALDLTHGELKVENKLWFTT
                     LDDYTLHAEVRVEGETLATQQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSR
                     TRYSEAGHSIATYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKM
                     SGKPTSWQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRDFAVEQ
                     SDGEVLIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFT
                     MGINGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNGNRQHVRW
                     TALTNRHGNGLLVVPQRPINFSAWRYTQENIHAAQHCNELQRSDDITLNLDHQLLGLG
                     SNSWGSEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ
                     "
     gene            complement(29048..30031)
                     /gene="ebgR"
                     /locus_tag="ACHKTW_000375"
     CDS             complement(29048..30031)
                     /gene="ebgR"
                     /locus_tag="ACHKTW_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HFO6461463.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLGIELTN
                     CYEHSGLPDIKNVTGILIVGKPTPALRAAACALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            30250..30582
                     /gene="ygjH"
                     /locus_tag="ACHKTW_000376"
     CDS             30250..30582
                     /gene="ygjH"
                     /locus_tag="ACHKTW_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HFO6461464.1"
                     /translation="METVAYADFARLEMRVGKIVEVKRHENADKLYIVQVDVGEKTLQ
                     TVTSLVPYYSEEELMGKTVVVLCNLQKAKMRGETSECMLLCAETDDGSESVLLTPERM
                     MPAGVRVV"
     gene            complement(30624..32003)
                     /gene="ygjG"
                     /locus_tag="ACHKTW_000377"
     CDS             complement(30624..32003)
                     /gene="ygjG"
                     /locus_tag="ACHKTW_000377"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0033094 - putrescine--2-oxoglutarate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HFO6461465.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGGLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGAFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            32421..33914
                     /gene="aer"
                     /locus_tag="ACHKTW_000378"
     CDS             32421..33914
                     /gene="aer"
                     /locus_tag="ACHKTW_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
                     /protein_id="HFO6461466.1"
                     /translation="MSSHPYVTQQNTPLADDTTLMSTTDLQSYITHANDTFVQVSGFT
                     LQELQGQPHNMVRHPDMPKAAFADMWFTLKKGEPWSGIVKNRRKNGDHYWVRANAVPM
                     VREGKISGYMSIRTRATDEEIAAVEPLYKALNAGRTSKRIHKGLVVRKGWLGKLPSLP
                     LRWRARGVMTLMFILLAAMLWFVAAPVVTYFLCVLVVLLASACFEWQIVRPIENVARQ
                     ALKVATGERNSVEHLNRSDELGLTLRAVGQLGLMCRWLINDVSSQVSSVRNGSETLAK
                     GTDELNEHTQQTVDNVQQTVATMNQMAASAADKLSITASNAAVQGGEAMTTVIKTMDD
                     IADSTQRIGTITSLINDIAFQTNILALNAAVEAARAGEQGKGFAVVAGEVRHLASRSA
                     NAANDIRKLIDASADKVQSGSQQVHAAGRTMEDIVAQVKNVTQLIAQISHSTLEQADG
                     LSSLTRAVDELNLITQKNAELVEESAQVSEMVKHRASRLEDAVTVLH"
     gene            complement(34068..34691)
                     /gene="nfeR"
                     /locus_tag="ACHKTW_000379"
                     /gene_synonym="yqjI"
     CDS             complement(34068..34691)
                     /gene="nfeR"
                     /locus_tag="ACHKTW_000379"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HFO6461467.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLHVNQSDISDAQIKKIIAVIDRAAFDITQLD"
     gene            34968..35732
                     /gene="nfeF"
                     /locus_tag="ACHKTW_000380"
                     /gene_synonym="yqjH"
     CDS             34968..35732
                     /gene="nfeF"
                     /locus_tag="ACHKTW_000380"
                     /gene_synonym="yqjH"
                     /EC_number="1.16.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase NfeF"
                     /protein_id="HFO6461468.1"
                     /translation="MNNSPRYPQRVRNDLRFRELTVLRVERISAGFQRIVLGGEALDG
                     FTSRGFDDHSKLFFPQPDAHFVPPTVTEEGIVWPEGPRPPSRDYTPLYDELRHELAID
                     FFIHDGGVASGWAMQAQPGDKLTVAGPRGSLVVPEDYAYQLYVCDESGMPALRRRLET
                     LSKLAVKPQVSALVSVRDNACQDYLAHLDGFNIEWLAHDEQAVDARLAQMQIPADDYF
                     IWITGEGKVVKNLSRRFEAEQYDPQRVRAAAYWHAK"
     gene            complement(36051..36132)
                     /locus_tag="ACHKTW_000381"
     tRNA            complement(36051..36132)
                     /locus_tag="ACHKTW_000381"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(36092..36094),aa:Tyr,seq:gta)
     gene            complement(36156..36250)
                     /locus_tag="ACHKTW_000382"
     tRNA            complement(36156..36250)
                     /locus_tag="ACHKTW_000382"
                     /product="tRNA-OTHER"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
     gene            complement(36530..36937)
                     /locus_tag="ACHKTW_000383"
     CDS             complement(36530..36937)
                     /locus_tag="ACHKTW_000383"
                     /inference="COORDINATES: protein motif:HMM:NF020345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mor transcription activator family protein"
                     /protein_id="HFO6461469.1"
                     /translation="MNEQTHHVPKDIERLKFIDAIDNGDKPGDAWCDSLADIVSHSVA
                     ELTRNGMEEADAFRIAKRVVAAIAFYAGGRSLYLPTGRSLFAALREYEIYYRWDNGEK
                     LQALRKEYAISETHIYDIIRRLRKKHANKITQD"
     gene            complement(37051..37320)
                     /locus_tag="ACHKTW_000384"
     CDS             complement(37051..37320)
                     /locus_tag="ACHKTW_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFO6461470.1"
                     /translation="MVHDSIAEELEAKGFYRRAAARWAEVMMLVETDRERERVAMRRA
                     ECYGKTQRPPEQPDDLGGLKRAIKRTEKEMGIDGRGKKIWRSYQK"
     gene            complement(37331..37741)
                     /locus_tag="ACHKTW_000385"
     CDS             complement(37331..37741)
                     /locus_tag="ACHKTW_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFO6461471.1"
                     /translation="MTAQIAAYGRLIDDPQTKQTSKGTPMTLARMAVSLPCSQAQDGQ
                     ATLWLSVLAFCKQADQLAGHEKGNVISVSGVMQVSQWTGQNGETRQGYQVIADSVISA
                     KTARPGGNRRRTTGTQGNQPPAGGDDPYGDNIPF"
     gene            complement(38181..39578)
                     /locus_tag="ACHKTW_000386"
     CDS             complement(38181..39578)
                     /locus_tag="ACHKTW_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase RepA family protein"
                     /protein_id="HFO6461472.1"
                     /translation="MKSAPNLKKQPYDKMTEVIIFAGSDAWAHAKQWQEQEGRLAGDN
                     VPPVWLGEQQLAELADTKIIDEGRYCVRLYKAGHIRPSNINAIAQKLAAAGVQDANYY
                     PDGMHSQKRENWREYLERERGEKQEEKGHQRKTKLPMSVGSAGYDTQLDYVVKGIIPA
                     VSLCSIYGASGSYKSFLAGSWACHVSTGRQWGGRRVAHGAVLYVVGEGGIGVPRRVKA
                     WEVVHGEQVKNLYLVNRPIFPAAPLDVDEMVIAARQVERETGKPVRMIILDTLARCFG
                     GNDENDSRDMGAFIRGCDELKRRTGATVLVVHHSGKDETKGARGSSAFRASLDAEYRI
                     RREDAGSEALVISCTKMKDAEELKEAAYDLRVVELFTDADGELITSLVVVDKPRHPVE
                     PERIEEAGNKTENHTALWGCIRSRTQNGDKCTIPLLRDDMKRLGYDVKNMRRWLAKLE
                     KDDVIYIDGDDVGPL"
     gene            complement(39575..39874)
                     /locus_tag="ACHKTW_000387"
     CDS             complement(39575..39874)
                     /locus_tag="ACHKTW_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFO6461473.1"
                     /translation="MNMKKTGLTASGKPRPEFKRGDIYRDQYGGTVMIKGVAGRCVTY
                     RRKGYEYDCVMPVYQFSRDFSLVQAVPRNVPTSNVKALAKIQKMKKMINAFRGKK"
     gene            complement(39947..40261)
                     /locus_tag="ACHKTW_000388"
     CDS             complement(39947..40261)
                     /locus_tag="ACHKTW_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6461474.1"
                     /translation="MRDVCNTRILEQLVTTIKAADAREARAMFDDADFYARKLLEGLA
                     ITGRLLSGMSDSIDPDMDELRSLGDSIAVTAELVAGFSEVVEVYNWRCRNGDISADYR
                     HS"
     gene            complement(40254..40718)
                     /locus_tag="ACHKTW_000389"
     CDS             complement(40254..40718)
                     /locus_tag="ACHKTW_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000916858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HFO6461475.1"
                     /translation="MLKNMLIASSFGLYDAGVVHMATGFSSLNNIADSRLHSDMRFFC
                     VRKPTHTRIMAGRNGGAFALAGFYVASLLTLLRLATPFSSVVARLLTSNIGAFHMAVS
                     ARPYFVWRFMQCHHSNIDVFTVTAATEHEARAQLPHAHLVFVARIRQGVRHA"
     gene            complement(40711..40899)
                     /locus_tag="ACHKTW_000390"
     CDS             complement(40711..40899)
                     /locus_tag="ACHKTW_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HFO6461476.1"
                     /translation="MATTPALVHSQTAFIWRFITFGTSERQIIHVTAWTEREARNRCP
                     SGCVAVFAARIRQGVSHA"
     gene            complement(40930..41256)
                     /locus_tag="ACHKTW_000391"
                     /pseudo
     CDS             complement(40930..41256)
                     /locus_tag="ACHKTW_000391"
                     /inference="COORDINATES: protein motif:HMM:NF022029.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
     gene            complement(41430..41633)
                     /locus_tag="ACHKTW_000392"
     CDS             complement(41430..41633)
                     /locus_tag="ACHKTW_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO6461477.1"
                     /translation="MNITEKKILTKREVANLLRLSTTSIDTMVKAGEFIPGFKRGLRR
                     VGWFEDEVKSWLEKKVEERYQQE"
     gene            complement(41772..42668)
                     /locus_tag="ACHKTW_000393"
     CDS             complement(41772..42668)
                     /locus_tag="ACHKTW_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016242744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6461478.1"
                     /translation="MANKKINLPERDYYPLDKAARRLGCDVDDLIHFIATGKMCFWYR
                     HHIDEALTLHNYKNAFNSVSASDYYYENDEGVFIRATDILKLLESSYFGEKITNNEDQ
                     LTEKVFFYYDSSYSETDIFICNYQKYKQRKIRTQLPHKIRVVASGLLQVFNTSIDIEN
                     LSNEITNCSVRRIVIADKAEEPEITINLYGKTINISDVFISKEEIKIVMNGGRQPGDM
                     VKDFSDKHALSHSQSKKTTNLQARVIKRLITAIAGEEEASNPRRALENGHSKLSRALT
                     KAGVDIGATPTCIGKWLADVDD"
     gene            complement(42673..43938)
                     /locus_tag="ACHKTW_000394"
     CDS             complement(42673..43938)
                     /locus_tag="ACHKTW_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001384119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase domain-containing protein"
                     /protein_id="HFO6461479.1"
                     /translation="MTKKVKSLSPATIKSLQRKASNEKKDLFITDGEGLELVIKHKSA
                     SCLWRYRYYKPHTKKRAMLSIGAYPEISIAGARKIKDEYRALLSGGIDPKEHREKQRL
                     SEVMKRENTFRHVATLWFELKKGQGLAEDTLRDIWRSLERNVFPSVGNRPIADLIASN
                     FIEALKPLAAQGKLDTVRRTAQRINEVMYYAVNTGMIAANQASEISKAFNAPSVKNMP
                     AIRPEELPEFMQKLSTARVERQTRLVIEWQLLTICRPGEAAGARWCEIDFEAKEWRIP
                     EHRLKQRREHVIPLSKQALAILDVMKPISGHREFIFPHNSDPQRPMCTQTANAAIKRM
                     GFGRRLVAHGLRSIASTALNEQGFDSDVIEAALSHIDKNEVRRAYNRSTYLEQRRPLM
                     AWWGNFVEAAASGSVSATGGYRGLKMVNG"
     gene            complement(44137..44212)
                     /locus_tag="ACHKTW_000395"
     tRNA            complement(44137..44212)
                     /locus_tag="ACHKTW_000395"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(44177..44179),aa:Ile,seq:cat)
     gene            44337..44843
                     /gene="mug"
                     /locus_tag="ACHKTW_000396"
     CDS             44337..44843
                     /gene="mug"
                     /locus_tag="ACHKTW_000396"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000700 - mismatch base pair DNA
                     N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HFO6461480.1"
                     /translation="MVEDILAPGLRVVFCGINPGLSSAGTGFPFAHPANRFWKVIYQA
                     GFTDRQLKPQEAQHLLDYRCGVTKLVDRPTVQANEVSKQELHAGGRKLIEKIEDYQPQ
                     ALAILGKQAYEQGFSQRGAQWGKQTLTIGSTQIWVLPNPSGLSRVSLEKLVEAYRELD
                     QALVVRGR"
     gene            complement(44921..46762)
                     /gene="rpoD"
                     /locus_tag="ACHKTW_000397"
     CDS             complement(44921..46762)
                     /gene="rpoD"
                     /locus_tag="ACHKTW_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFO6461481.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSNAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
     gene            complement(46957..48702)
                     /gene="dnaG"
                     /locus_tag="ACHKTW_000398"
     CDS             complement(46957..48702)
                     /gene="dnaG"
                     /locus_tag="ACHKTW_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFO6461482.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQKPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            complement(48813..49028)
                     /gene="rpsU"
                     /locus_tag="ACHKTW_000399"
     CDS             complement(48813..49028)
                     /gene="rpsU"
                     /locus_tag="ACHKTW_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFO6461483.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            49266..50279
                     /gene="tsaD"
                     /locus_tag="ACHKTW_000400"
     CDS             49266..50279
                     /gene="tsaD"
                     /locus_tag="ACHKTW_000400"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFO6461484.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            complement(50322..51785)
                     /gene="ttdT"
                     /locus_tag="ACHKTW_000401"
     CDS             complement(50322..51785)
                     /gene="ttdT"
                     /locus_tag="ACHKTW_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HFO6461485.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALLPVPAGLENHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALFLVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVALIVVFYLLRY
                     FFASATAYTSALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTLYATGPSPIYYG
                     SGYLPTVDYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(51833..52438)
                     /gene="ttdB"
                     /locus_tag="ACHKTW_000402"
     CDS             complement(51833..52438)
                     /gene="ttdB"
                     /locus_tag="ACHKTW_000402"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HFO6461486.1"
                     /translation="MKKILTTPIKAEDLQDIRVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            complement(52435..53346)
                     /gene="ttdA"
                     /locus_tag="ACHKTW_000403"
     CDS             complement(52435..53346)
                     /gene="ttdA"
                     /locus_tag="ACHKTW_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008730 - L(+)-tartrate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
                     /protein_id="HFO6461487.1"
                     /translation="MMSESNKQQAVNKLTEIVANFTAMISTRMPDDVVDKLKQLKDAE
                     TSSMGKIIYHTMFDNMQKAIDLNRPACQDTGEIMFFVKVGSRFPLLGELQSILKQAVE
                     EATVKAPLRHNAVEIFDEVNTGKNTGSGVPWVTWDIIPDNDDAEIEVYMAGGGCTLPG
                     RSKVLMPSEGYEGVVKFVFENISTLAVNACPPVLVGVGIATSVETAAVLSRKAILRPI
                     GSRHPNPKAAELELRLEEGLNRLGIGPQGLTGNSSVMGVHIESAARHPSTIGVAVSTG
                     CWAHRRGTLLVHADLTFENLSHTRSAL"
     gene            53553..54485
                     /gene="ttdR"
                     /locus_tag="ACHKTW_000404"
                     /gene_synonym="dan"
     CDS             53553..54485
                     /gene="ttdR"
                     /locus_tag="ACHKTW_000404"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate utilization transcriptional activator
                     TtdR"
                     /protein_id="HFO6461488.1"
                     /translation="MLNSWPLAKDLQVLVEIVHSGSFSAAAATLGQTPAFVTKRIQIL
                     ENTLATTLLNRSARGVALTESGQRCYEHALEILTQYQRLVDDVTQIKTRPEGMIRIGC
                     SFGFGRSHIAPAITELMRNYPELQVHFELFDRQIDLVQDNIDLDIRINDEIPDYYIAH
                     LLTKNKRILCAAPEYLQKYPQPQSLQELSRHDCLVTKERDMTHGIWELGNGQEKKSVK
                     VSGHLSSNSGEIVLQWALEGKGIMLRSEWDVLPFLESGKLVQVLPEYAQSANIWAVYR
                     EPLYRSMKLRVCVEFLAAWCQQRLGKPDEGYQVM"
     gene            complement(54498..55115)
                     /gene="plsY"
                     /locus_tag="ACHKTW_000405"
     CDS             complement(54498..55115)
                     /gene="plsY"
                     /locus_tag="ACHKTW_000405"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFO6461489.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPIGWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            55220..55588
                     /gene="folB"
                     /locus_tag="ACHKTW_000406"
     CDS             55220..55588
                     /gene="folB"
                     /locus_tag="ACHKTW_000406"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HFO6461490.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            55678..56499
                     /gene="bacA"
                     /locus_tag="ACHKTW_000407"
     CDS             55678..56499
                     /gene="bacA"
                     /locus_tag="ACHKTW_000407"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFO6461491.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMLWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(56662..57900)
                     /gene="cca"
                     /locus_tag="ACHKTW_000408"
     CDS             complement(56662..57900)
                     /gene="cca"
                     /locus_tag="ACHKTW_000408"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HFO6461492.1"
                     /translation="MKIYLVGGAVRDALLGLPVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIHTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREAWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPD"
     gene            complement(57964..58584)
                     /gene="ygiM"
                     /locus_tag="ACHKTW_000409"
     CDS             complement(57964..58584)
                     /gene="ygiM"
                     /locus_tag="ACHKTW_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04211 family SH3 domain-containing protein"
                     /protein_id="HFO6461493.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPTRKRKDRWMN"
     gene            58826..60127
                     /gene="ygiF"
                     /locus_tag="ACHKTW_000410"
     CDS             58826..60127
                     /gene="ygiF"
                     /locus_tag="ACHKTW_000410"
                     /EC_number="3.6.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HFO6461494.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRGHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEV
                     WPNGELPADLASRVQPLFSTDFYREKWLVAVDDSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELALAQWQYHEELWVRGNDAAKEQVLAAIGLVRHALMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQSKM
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYRDQLPRLMRDIDSILLLAGYYDPVVAQ
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            60150..62990
                     /gene="glnE"
                     /locus_tag="ACHKTW_000411"
     CDS             60150..62990
                     /gene="glnE"
                     /locus_tag="ACHKTW_000411"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HFO6461495.1"
                     /translation="MKPLSSPLQQYWQTVVERLPEPLAEKSLSAQAKSVLTFSDFVQD
                     SVIAHPEWLTELESQSPQADEWQHYAAWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSHLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPSLQSRSLLPTLSAIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMTNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTLIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFRAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWVQMVARYGKPNHLNDREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSAEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRNRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVSEDCFTAERDLVRASWQKWLVEE"
     gene            63038..64471
                     /gene="hldE"
                     /locus_tag="ACHKTW_000412"
     CDS             63038..64471
                     /gene="hldE"
                     /locus_tag="ACHKTW_000412"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HFO6461496.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVSYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(64591..64650)
                     /gene="ibsE"
                     /locus_tag="ACHKTW_000413"
     CDS             complement(64591..64650)
                     /gene="ibsE"
                     /locus_tag="ACHKTW_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsE"
                     /protein_id="HFO6461497.1"
                     /translation="MMKFVIILVVLLLLSFPTY"
     gene            complement(64967..65026)
                     /locus_tag="ACHKTW_000414"
     CDS             complement(64967..65026)
                     /locus_tag="ACHKTW_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFO6461498.1"
                     /translation="MMKLVIILIVLLIVSFAAY"
     gene            complement(65265..66926)
                     /gene="yqiK"
                     /locus_tag="ACHKTW_000415"
     CDS             complement(65265..66926)
                     /gene="yqiK"
                     /locus_tag="ACHKTW_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HFO6461499.1"
                     /translation="MDDIVNSVPSWMFTAIIAVCILFIIGIIFARLYRRASAEQAFVR
                     TGLGGQKVVMSGGAIVMPIFHEIIPINMNTLKLEVSRSTIDSLITKDRMRVDVVVAFF
                     VRVKPSVEGIATAAQTLGQRTLSPEDLRMLVEDKFVDALRATAAQMTMHELQDTRENF
                     VQGVQNTVAEDLSKNGLELESVSLTNFNQTSKEHFNPNNAFDAEGLTKLTQETERRRR
                     ERNEVEQDVEVAVREKNRDALSRKLEIEQQEAFMTLEQEQQVKTRTAEQNARIAAFEA
                     ERRREAEQTRILAERQIQETEIEREQAVRSRKVEAEREVRIKEIEQQQVTEIANQTKS
                     IAIAAKSEQQSQAEARANLALAEAVSAQQNVETTRQTAEADRAKQVALIAAAQDAETK
                     AVELTVRAKAEKEAAEMQAAAIVELAEATRKKGLAEAEAQRALNDAINVLSDEQTSLK
                     FKLALLQALPAVIEKSVEPMKSIDGIKIIQVDGLNRGSAAGDANTGNVGGGNLAEQAL
                     SAALSYRTQAPLIDSLLNEIGVSGGSLAALTSPLTSTTPVAENVE"
     gene            complement(66953..67582)
                     /gene="yqiJ"
                     /locus_tag="ACHKTW_000416"
     CDS             complement(66953..67582)
                     /gene="yqiJ"
                     /locus_tag="ACHKTW_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold-containig protein"
                     /protein_id="HFO6461500.1"
                     /translation="MILFADYNTPYLFAISFVLLIGLLEILALICGHMLSGALDAHLD
                     HYDSITTGHISQALHYLNIGRLPALVVLCLLAGFFGLIGILLQHACIMVWQSPLSNLF
                     VVPVSLLFTIIAVHYTGKIVAPWIPRDHSSAITEEEYIGSMALITGHQATSGNPCEGK
                     LTDQFGQIHYLLLEPEEGKFFTKGDKVLIICRLSATRYLAENNPWPQIL"
     gene            67851..68051
                     /gene="glgS"
                     /locus_tag="ACHKTW_000417"
     CDS             67851..68051
                     /gene="glgS"
                     /locus_tag="ACHKTW_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HFO6461501.1"
                     /translation="MDHSLNSLNNFDFLARSFARMHAEGRPVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQARELELEH"
     gene            complement(68094..69158)
                     /gene="yqiI"
                     /locus_tag="ACHKTW_000418"
     CDS             complement(68094..69158)
                     /gene="yqiI"
                     /locus_tag="ACHKTW_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO6461502.1"
                     /translation="MRYLLIVIIFIIGFSALPVWAMDCYAEHEGGNTVVIGYVPRISI
                     PSNGKKGDKIWQSSEYFMNVFCNNALPAPSPGEEYPSAWTNIMMFLPGGQDFYNQNSY
                     IFGVTYNGVDYDSTAPNAIAAPECIDIKGAGTFNNHYKNPAVCSGGPEPQLSVTFPAR
                     VQLYIKLAKNANRVNKNMVLPDEYIALEFKGMSGAGAIEVDKNLTFRIRGLNNIHVLD
                     CFVNVALEPADGVVDFGKINSRTIKNTSVSETFSVVMTKDPGAACTEQFNILGSFFTT
                     DILSDYSHLDMGNGLLLKIFHKDGTATEFNRFSQFASFSSSSAPSVTAPFRAELSANP
                     AETVVEGPFSKDVILKITYN"
     gene            complement(69160..69909)
                     /gene="yqiH"
                     /locus_tag="ACHKTW_000419"
     CDS             complement(69160..69909)
                     /gene="yqiH"
                     /locus_tag="ACHKTW_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFO6461503.1"
                     /translation="MRYLNTKNLIAAGVLLACMSSIAWGAIIPDRTRIIMNESDKGEA
                     LKLTNQSKNLPYLAQTWIEDTKGNKSRDFIVTVPPMVRLNPSEQIQIRMISQEKIAQL
                     PNDRETLFYFNVREIPPKTDKKNVMQVTMQHALKLFWRPKAIELEDDGVMSYEKVEII
                     RRNDGSIRFNNKMPYHVTLGYIGTNGITMLPQTQSLMVTPYSHADTQFKNVPSAFQVG
                     YINDFGGLSFYEINCPTVNNSCNVSVAKRDK"
     gene            complement(69925..72429)
                     /locus_tag="ACHKTW_000420"
     CDS             complement(69925..72429)
                     /locus_tag="ACHKTW_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFO6461504.1"
                     /translation="MYKKLKLTTISELIKNIYCSLSVLIIGCASAYAVEFNKDLIEAE
                     DRENVNLSQFETDGQLPVGKYSLNALINNKRTPIHLDLQWVLIDNQTAVCLTPEQLTL
                     LGFTDEIIEVAQQNLIDGCYPIEKEKQITTYLDKGKMQLSISAPQAWLKYKDANWTPP
                     ELWDHGIAGAFLDYNLYASHYAPHQGDNSQNISSYGQAGVNLGAWRLRTDYQYDQSFN
                     NGKSQANNLDFPRIYLFRPIPAINAKLTIGQYDTESSIFDSFHFSGVSLKSDENMLPP
                     DLRGYAPQITGVAQTNAKVTVSQNNRIIYQENVPPGPFAITNLFNTLQGQLDVKVEEE
                     DGQVTQWQVASNSIPYLTRKGQIRYTTAMGKPTSVGGDSLQQPFFWTGEFSWGWLNNV
                     SLYGGSVLTNRDYQSLAAGVGFNLNSLGSLSFDVTRSDAQLHNQDKETGYSYRANYSK
                     RFESTGSQLTFAGYRFSDKNFVTMNEYINDTNHYTNYQNEKESYIVTFNQYLESLRLN
                     TYVSLARNTYWDASSNVNYSFSLSRDFDIGPLKNVSTSLTFSRINWEEDNQDQLYLNI
                     SIPWGTSRTLSYGMQRNQDNKISHTASWYDSSDRNNSWSVSASGDNDEFKDMKASLRA
                     SYQHNTENGRLYLSGTSQRDSYYSLNASWNGSFTATRHGAAFHDYSGSADSRFMIDAD
                     GAEDIPLNNKRAVTNRYGIGVIPSVSSYITTSLSVDTRNLPENVDIENSVITTTLTEG
                     AIGYAKLDTRKGYQIMGVIRLADGSHPPLGISVKDKTSHKELGLVADGGFVYLNGIQD
                     DSKLTLRWGDKSCFIQPPNSSNLTTGTVILPCISQN"
     gene            complement(72489..73040)
                     /gene="ygiL"
                     /locus_tag="ACHKTW_000421"
     CDS             complement(72489..73040)
                     /gene="ygiL"
                     /locus_tag="ACHKTW_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HFO6461505.1"
                     /translation="MSAFKKSLLVAGVAMILSNNVFADEGHGIVKFKGEVISAPCSIK
                     PGDEDLTVNLGEVADTVLKSDQKSLAEPFTIHLQDCMLSQGGTTYSKAKVTFTTANTM
                     TGQTDLLKNTKETEIGGATGVGVRILDSQSGEVTLGTPVVITFNNTNSYQELNFKARM
                     ESPSKDATPGNVYAQADYKIAYE"
BASE COUNT        17504 a        18373 c        18807 g        18357 t
ORIGIN      
        1 ggcgaaaatt aatctcttat cacggcttac gccgctttta ttcgtgttcg ctcctttttt
       61 ggcacagtca aatatgactg tttatcctat ggctgtttcc attaatagtc agggtgaagg
      121 caacgttcgc gtaatatcta aaagtaatga agttcagtac atcaaggcga cagtattccg
      181 tatcgataat ccatcgacgc ctcaggaaaa tgaagttgag attaaatccg gtgatgcaaa
      241 tcatctggtt gttatgccac ctaaattcgc tttaccagcc ggtagtagca aaaccgtacg
      301 ttttgttgcg atggaaccag agcaaaaaga gaaaaattat cgcgttaaat ttgaagcggt
      361 tcccagtatt gatgacgttg ccacagataa aaaagatctc tctatgcagt taacagttaa
      421 cttaatttgg ggaatcgttg ttagtgttcc acctcagcaa cctatagcta agttagaagt
      481 aaattctgcc caaaaattaa ttaatgcagg gaatcaacgc ttaaagcttt taacgatcgc
      541 ttattgtaaa aataatagca aagaaaactg taagatacag accgtaaata aaaacatctt
      601 ccctggtcag gaaagaaatc ttgaaagcat atctggctac gacaaaatcg ttgtcaaata
      661 taataactgg atcaccaaag ataatggcga gtttgaactg gcggtccatt aattatcatt
      721 gtaataaacg aaggaatagt aatgaaaaag gtatttgcaa aatctcttct ggtcgcagcg
      781 atgttttctg ttgcaggctc cgcattggca gtacaaaagg atattactgt aacggccaac
      841 gttgatgccg ctctggatat gacacagacc gataacaccg cgttgccgaa agcagtagaa
      901 atgcaatatc tgctggggca gggtctgcaa tcttaccagt tgatgaccaa aatctggtct
      961 aacgacgtta ccaaagatgt aaaaatgcag ttagtttccc cggcgcaact ggttcaaagc
     1021 ctggatgcta gcaagattgt tccgctgaca gtaacctggg gcggtgaaga aattaaagct
     1081 gatgcggcaa ccacttttac cgcaaccaaa atctttgctt ccgatgcgtt aactaacggt
     1141 tctctggcta aaaacctgat gtttgctcaa accaccaaag gtgttctgga gacaggcatc
     1201 taccgtggtg tagtaagcat ttatctgtct caggatatct aatccagata acagaattaa
     1261 cacgtaacaa agggaggggt agccctccct tattttaact gttttagtat ttgttatgga
     1321 tttcaggatt gccatggata aaaaattact ggctcttttg atcctggcga gtctcagccc
     1381 ggcagaggcg acattaacca aaattcccgc agggtttgag gttattgctc agggacagca
     1441 ggagtatatc gaggtttatt tttcagggaa aagtctcggt aaatattatg caatggttaa
     1501 tcttgatacc gtaacatttc ttgatccagc aagtctatat aacaagctgg aactggatgt
     1561 agacgatcag aaaatcgcgc atatagtgaa agaaaaatta tcgcagccgc tagctcgcca
     1621 cggtgaattg gcttgcggtt atgtacgtac tgactcaggg tgtggttttc tgaataccga
     1681 tacgctggaa ataatctata atgatgaaga aagttcggca acgttgttta ttaatccgca
     1741 atggaattca gctttcgatg cgaagtcatt atatttaaat ccagacaaaa atactgttaa
     1801 tgcttttata catcagcaag acatcaatgt tctggcacag gatgattacc aatcgttgtc
     1861 tattcaggga aacggtgcgc tgggaataac agaaaatagt tatattggtg cacactggaa
     1921 tttcaacggt tatgatgcag atgatgtcag tgacagtaat gctgacgtca gcgatctcta
     1981 ttatcgttat gattttttac gtcgttatta tgtgcaggcg ggtcgcatgg acaaccgcac
     2041 actatttaat gcacaaggcg ggaactttac ctttaacttt ctgccactcg gtgcaattga
     2101 tgggatgcgt atcgggtcga cactgagcta tttaaaccag gcgcaaagcc agcaaggaac
     2161 cccagtaatg gttctgcttt cgcgcaattc tcgtgttgac gcttatcgta atgagcaact
     2221 tttgggatcg ttttatctca atagtggttc gcaatttatt gataccagtt cctttccgcc
     2281 aggcagctac agcgtggcat taaaagtcta cgaaaataac caactcaccc gcaccgagct
     2341 tgtgccgttt accaaaaccg gcggtctgac tgacggaaat gcgcaatggt tcttacaggc
     2401 aggtaaaact acatcacagg tttctgatga tgaaagttca gcttatcagc taggagtacg
     2461 cctgccatta catccgcaat atgagctcta cgcagggctg gcgaatgccg atgatgtgag
     2521 tgctttcgag ttaggtaata actggacggc agatttaggc ggggtgggga atcttgcaat
     2581 cagcgccagc gtgttccgta acgatgacgg cggcaaaggt gatatgcaac aggccaactg
     2641 gagtaatccg ggatggccga cgttgggctt ttatcggacc aactctgacg gcgatgcttg
     2701 tacaaccgac agcagagaga gctataacgc cttaagctgt tatgaaagta tttccgcgac
     2761 ggtttcacag aattttgtcg gctggaatat gatgctgggt tatacccgca cacaaaataa
     2821 cactgatgat agtttgcgtt gggataaaca gcagagcttt gaaaataact atcttcgcca
     2881 gacaacagcg caaagtatct ctgaaactgt acaacttagc gcttcccgcg cttttgtgat
     2941 gcgtgactgg attttgagta cgtcagtcgg tgttttccat cgtaatgaca acggtggcga
     3001 taacgacgac aacggcttgt acttatcgtt ttcgttatct gacacgccaa cgatggatag
     3061 caataacaac agccattcaa caaatgtgtc tacggattat cgttatagcg aacaggatgg
     3121 cgatcaaacg tcatggcagt tatcgcatac cttttataac gattcattca gccataaaga
     3181 acttggcgta accgtcggag gcctgaacac cgataccata aacagcgcgg ttaacgggcg
     3241 ttgggatggg caatacggaa atgtctacgc taccgtatct gatagttatg accgtaagaa
     3301 tcatgatcat ctttcggcct ttacgggcac ttatagctct acgctggctg tcagtcgcta
     3361 tggcgttaat ttgggagcca gtggtacaga cgatttgctg ggtgcggtac tggtggatgt
     3421 gaaaggcttc tctgaacagg atgaagagag tcaggatctg caactcgaag cgcgggtggc
     3481 aggcagccga actttgcagc ttggtcaaag tgacagtgtg ttgttccctt atcctggatt
     3541 tcagtctggt tttgttgagg ttaacgacag tagccagggc aatcagcagg ggacaacaaa
     3601 catcattaac ggtgcaggga atcgtgaatt aatgttgttg cctggcaagc tgcgctatcg
     3661 cgaagtgtct gccagcttta actacaacta tatcggtcgt ttattactgc cggcagcggt
     3721 gaaaaaattc ccgattgtgg ggctgaacag cgccatgtta ctggtagctg aagatggcgg
     3781 atttacactt gaaattaacg gcagcgaaaa agagctctat ctgctttccg ggcagcaatt
     3841 ccttaagtgt ccactgagtg ttgtaaagaa acgcgccagc attcgttaca gcggagatgt
     3901 tacttgtagt gtggtgactt attcacaatt accggagtcg attcaggttc aggcacagtt
     3961 gaaacagcct aaattacgtg gaaacgttca gacggcgcaa agggaggttg caccatgaga
     4021 aatcgattga ttgcggcgat attgggcttg tttggcacgc tcactggcgt tcaggcagct
     4081 cctgacgtga ccagtgaaat tacgtatgat ttggcatctg gtagagcgga ttattacttc
     4141 tggaaggatg aagcgtcggc aggaaataat ggatatatgt ggtatgaatg ttcgtatcct
     4201 gacctccaac aaacctgtac agctaatgga aatatatcga cagtacaaat ctatttaact
     4261 gaacaacgca gtgggatgcg ttggccggta aaactcaaag gatttaaaac agccattgta
     4321 agtagcgatg aagcgccgcc aggatgcaag gggggcaaag ggcttcagac gaatcttaag
     4381 gattctaata gatcttcatg tacagaagat ggtcaacatt attatatata cgatacaaag
     4441 tttcttacgc tctaccttga gcagacagag atgaagaatt tgccgattgg tggcgtctgg
     4501 aaggggaaag ttaaattaca ttcgaacagc ccggcccagg actatttcgc aaatattacc
     4561 ctgaatacgc tcgaccccaa ccatattgac gtgttcttcc cggagttcgc ccacgccacg
     4621 ccaagggtgc agttagactt gcatccaaca ggaagcgtta acggcagcaa ctacgcgcaa
     4681 gatctgacca tgttggacat gtgcctgtac gatggtttta acggtaatgc catcagttat
     4741 gaaatcatgc tcaaagatga agggcgacca gctgcagggc gcagagacgg ttacttctct
     4801 atctatcgtc agggagggac caccaccgac gagggagaac gcattgatta ccgggtcaaa
     4861 atgtacaacc cggaaaccgg tgggcaaatt gatgtgcgca ataatgaaaa tatggtctgg
     4921 aacagcatta acctgaaacg tgtgcgtccg gtggtactgc ccggtattcg ctatgccgtg
     4981 atgtgtgtgc cgacaccctt gactctggca gtcgataaat tcagcgtgat ggataaacag
     5041 gccgggtact acatgggcaa attgtcagta atctttacgc cttccttgcc aaccatcaat
     5101 taaccctgca aaccggacaa acgctttttt ctatataaac gacgaagccg tctgataagg
     5161 cggcttcgtc attacaactt aagttaaacg ttaatgttgc ggtgcgggat cgttatgtgt
     5221 ttcctgcgcc acctcaatcg ccggtttgtt ctgcaacacg gtccagataa ccagcgcacc
     5281 taacaggtca aatactgcca gaactgcgaa cagcgggctg aagccgatgg tgtcagccag
     5341 tgcaccgaca accagcgcaa acagcgtact tgccagccat gcggacatcc cggttaaacc
     5401 gttcgccgtc gccacttcgt tacgaccgaa cacgtcagaa gagagcgtaa tcagcgcgcc
     5461 agacagtgcc tggtgggcaa aaccaccgat acacagcagc ataattgcga catacgggtt
     5521 ggtgaacaga ccgatcatac ccgggccaat catcagcaca gcacccagcg ttacgaccat
     5581 cttacgggaa acgatcaggt tcacaccaaa ccaacgctgg aacagcggcg gcaggtaacc
     5641 accgaggata caaccgaggt cagcaaacag catcggcatc caggcgaaca tcgcgatctc
     5701 tttcaggtta aagccgtaaa ctttaaacat gaacagcggg atccacgcgt taaaagtacc
     5761 ccaggccggt tctgccagga aacgcggcag cgcgataccc cagaactgac ggttacgcag
     5821 gatctgacca acggacattt tcttcgccgt gctcacctgg tgctgggctt cctgaccatt
     5881 aataatatag tcgcgttctt catcggtcag atgcttctgg tcgcgtggat gtttatagaa
     5941 aatcagccat gccatcgccc agataaagct caatgcaccg gagatgataa atgccatctg
     6001 ccagctgtgc attacgattg cccataccac cagcggcggc gcaatcatcg caccaatcga
     6061 agaacctacg ttaaagtaac ctaccgcgat ggaacgctct ttcgccggga accattcgga
     6121 gctggctttc agacccgccg ggatcatcgc tgcttccgcg gcaccgactg caccacgagc
     6181 aatcgccagg ccaccccagc taccagccag cgcggttgca ccacagaaca cggcccacag
     6241 cacagcaaac attgcataac cgattttcgt acccagcaca tccagtacat agcccgctac
     6301 cggttgcatg accgtataag cagcagaata ggctgcgatg atataggaat actgttgggt
     6361 ggagatgttt aactcttcca tcagagttgg cgcagctgcc gccacagtgt tacgcgtcag
     6421 gtaaccaagc acggtgccaa gcgtcaccag tgcgatcata taccaacgta accctttaat
     6481 tttacgcatg taaaacctca tcgttttgtt atatccgcag gaagcgcggc catcatcccg
     6541 ccagaggctg aagatgttag ttacgaccgg gtaaccgaaa aaaaaccggc acgccccgaa
     6601 cgttgccatg aaagtttcgt gctaattcgg cttccgtacc ggttaacccg attcatctaa
     6661 caaatgaatg aatcggcaat ttgcattccg tcgacttatg atttgcgacg gcagaaagat
     6721 aacttgtcat acaactttaa aaggtgagag ccatcacaaa tgtgcgaata tttgtaggga
     6781 cattacctga cgacagcgag gccagtactg gcgcggcctg cagagagatt taccactttg
     6841 agagtaattt ttttaactac gtttattgat ctaactcacg aaaatatctt cggactctgg
     6901 aaattggtgt gataactttg tcagcatcgc accataagca agctagctca ctcgttgaga
     6961 ggaagacgaa aatgactccg tttatgactg aagatttcct gttagatacc gaatttgccc
     7021 gccgtctgta tcacgactac gcaaaagacc agccgatttt cgattaccat tgccatttgc
     7081 cgccgcagca gattgcggaa gactatcgtt ttaaaaacct gtatgacatc tggctgaaag
     7141 gcgatcacta caaatggcgc gctatgcgta ccaacggtgt ggccgagcgt ctgtgtaccg
     7201 gtgatgcgtc tgaccgtgaa aaatttgacg cctgggcggc gactgttccg cacactatcg
     7261 gcaacccgtt ataccactgg acgcacctcg aactgcgtcg tccgtttggt atcactggca
     7321 aattgctttc tccgtcaact gccgatgaaa tctggaacga atgtaacgaa ctgctggcgc
     7381 aggataactt ctccgcgcgc ggcatcatgc agcagatgaa cgtgaaaatg gtcggcacca
     7441 ccgatgaccc gatcgattct ctggagcatc acgcagagat cgccaaagac ggctctttca
     7501 ccatcaaagt gctgccgagc tggcgtccgg acaaagcctt caacatcgaa caggcgacct
     7561 ttaacgacta catggcgaag ctgggcgaag tttccgatac cgacattcgc cgctttgctg
     7621 acctgcaaac tgccttgacc aaacgtctgg atcacttcgc cgctcacggc tgtaaagtgt
     7681 ctgaccatgc gctggacgtg gtgatgtttg ctgaagcgaa cgaagcggaa ctggacagca
     7741 ttctggcgcg ccgtctggct ggcgaacccc tgagcgagca cgaagtggca cagttcaaaa
     7801 ctgcggtact ggtgttcctt ggtgccgaat atgcacgtcg cggctgggta cagcagtacc
     7861 acattggcgc gctgcgtaat aacaacctgc gtcagttcaa actgctgggg ccggatgtag
     7921 gctttgactc catcaacgac cgtccgatgg cggaagagtt gtctaagctg ctgagcaagc
     7981 agaacgaaga aaacctgctg ccgaaaacca ttctctactg cctgaacccg cgcgataacg
     8041 aagtgctggg caccatgatc ggtaacttcc agggcgaagg tatgccgggc aaaatgcagt
     8101 tcggttccgg ctggtggttt aacgatcaga aagacggtat ggaacgtcag atgacccaac
     8161 tggcgcagct cggtctgctg agccgctttg tcggtatgct gactgacagc cgtagcttcc
     8221 tgtcatacac ccgtcacgaa tacttccgcc gcattctgtg ccagatgatc ggtcgctggg
     8281 tggaagcggg cgaagcaccg gcggacatca acctgctggg cgagatggtg aaaaatattt
     8341 gctttaacaa tgcgcgtgac tacttcgcca ttgaactgaa ctaaggtctg gggttgatat
     8401 gcaatacatc aagatccatg cgctggataa cgtcgcggtc gctttagcag atttggctga
     8461 aggcacagaa gtcagtgtcg ataaccagac tgttacgctg cgccaggatg ttgctcgtgg
     8521 acataaattt gcgttaacgg atatcgcaaa aggggccaac gtcatcaaat atggcctgcc
     8581 gattggttat gcattggcgg atattgcggc aggtgttcac gttcacgccc acaatacgcg
     8641 cacgaatctg agcgatctgg atcagtatcg ctatcaacct gattttcagg atctgcctgc
     8701 gcaagcggca gatcgtgaag tgcagatcta tcgtcgcgct aacggcgatg tcggggtgcg
     8761 taatgagctg tggatcctgc caaccgtggg ctgtgtcaac ggcatcgcgc ggcaaatcca
     8821 gaaccgtttc ctgaaagaga ccaacaacgc cgaaggtacc gacggcgtgt tcctcttcag
     8881 ccacacctac ggctgctcac aactgggcga cgatcacatc aacacccgca ccatgctgca
     8941 aaacatggtg cgccacccga acgcgggcgc agtgctggtg attggtctgg gctgtgaaaa
     9001 caaccaggtt gccgcattcc gtgaaacgtt gggcgatatc gatcctgaac gcgttcattt
     9061 catgatctgc caacagcagg atgatgagat cgaagccggg atcgagcatt tgcatcagct
     9121 gtataacgtg atgcgcaacg ataaacgcga gccaggcaaa ctcagcgaac tgaagtttgg
     9181 tctggagtgc ggtggttctg acggtctttc tggtattact gctaacccga tgctggggcg
     9241 tttctctgac tacgtgattg ctaacggcgg tactaccgta ctgaccgaag tgccggagat
     9301 gtttggcgca gagcagttgc tgatggacca ttgccgcgac gaagcaacgt ttgaaaaact
     9361 ggtcaccatg gtcaacgact tcaaacagta ctttattgcc catgaccagc cgatctatga
     9421 aaacccatcg ccggggaaca aagcgggcgg tatcaccacg ctggaagata aatcccttgg
     9481 ctgtacccag aaagcgggtt ccagcgtcgt ggttgacgtg ctgcgttacg gcgagcgtct
     9541 gaaaacgccg gggctgaact tgttaagtgc gccgggtaac gatgccgtag cgaccagcgc
     9601 cctggcgggt gcgggctgcc atatggtgct gttcagtact ggtcgtggca cgccatacgg
     9661 cggctttgta ccgacggtga aaatcgctac caacagtgaa ctggcggcga agaaaaaaca
     9721 ctggatcgac tttgacgcgg gtcagctgat ccacggtaaa gcgatgccgc agttgctgga
     9781 agaatttatc gacaccatcg ttgagtttgc caacggtaaa caaacctgca acgagcgtaa
     9841 cgacttccgc gaactggcga tcttcaaaag cggcgtaacg ctataaagtc gaaatgagtt
     9901 gttaataaaa aacggcgttt cataatgtga agcgccgttt gcttttccat acctgataag
     9961 gatttatcat gaccgcgtat tggctggccc agggcgtggg tgtcatcgcc tttctgattg
    10021 gtatcacaac atttttcaat cgtgacgaac gtcgcttcaa aaagcagctt tcggtctata
    10081 gcgccgttat tggcgtacat ttttttcttc tgggcaccta tcccgctggt gccagtgcca
    10141 tccttaatgc cattcgtaca ttgattacct tacgcacgcg cagcttatgg gtaatggcga
    10201 tttttattgt gctgactggc ggaattggcc tcgcgaagtt ccatcatcct gtcgaactat
    10261 tgccggttat cggtacgatt gtcagtacct gggcgctgtt ccgctgtaaa gggctcacca
    10321 tgcgctgcgt gatgtggttt tcaacgtgtt gctgggtgat tcacaacttc tgggcggggt
    10381 cgataggcgg cacaatgatt gagggtagtt ttctgcttat gaatggcttg aatatcattc
    10441 gtttctggcg gatgcagaaa aggggaattg atccgtttaa agtagagaaa acccctcccg
    10501 ccgtagacga aaggggttaa acaattaatt acgcagggcg ctatttgcca gacgatcgtc
    10561 ttccgcctgg caagctgccg cagtgaacag cacgtcagtt gaagagttca gcgcggtttc
    10621 gcaagagtcc tgcaatacgc cgatgataaa gccgacggca accacctgca tggcgatatc
    10681 gttcgaaata ccgaacatat tacaggccag cgggatcagc agcagagaac cccccgccac
    10741 gccggatgcg ccacaggcac acagagaagc caccacgctc aacagcagcg ccgtgggcag
    10801 atcgaccggg atacccagcg tattaaccgc agccagcgtc aacacggtaa tggtgattgc
    10861 tgcgcccgcc atattgatgg tggcacccag cggaatagaa acggaatagg tatcgcgatc
    10921 cagattcagc ttttcacaca gtgccatatt caccggaatg ttcgccgcag agctgcgggt
    10981 gaagaaggca tacacgccgc tttcgcgcag gcacagcagc accagcggga acgggttacg
    11041 acgaattttc caccacacca gcaatgggtt aaccaccagc gccaccagta acatacagcc
    11101 aaccagcacg accagcagtt gcgcgtagcc ccacagtgtg gagaaaccgg tggttgccag
    11161 ggtagaagaa accagaccaa aaataccaat cggtgcgaag cgaatgacca gtttcaccat
    11221 aaaggtgacg gcattcgaca tatcgttgac caggtttttg gtggtctcgt taccgtgacg
    11281 cagtgcgaaa ccgaggccaa ttgcccacac caggatcccg atgtagttac ctttcagcag
    11341 cgcgtcgatg gggttggaaa ccatgctcat taccagcccg cgcatcactt cgacaatgcc
    11401 tgacggcggc gaaatatcac ccgcgctact ggataaatgc agggtagaag ggaaggcaaa
    11461 gctgaagact actgcggcca gagcagcaga gaaggtaccc agcagataga ggaacaaaat
    11521 agggcggata ttggttttct gcccgtgctg gtggttagca atagatgcca tcaccagcat
    11581 caacaccagg atgggggcaa cggctttcag tgcgccgacg aacaaagtac ctaacagacc
    11641 aacagcttcc gccgcgggtt ttgagatcca tgccagaaga atccccagaa caaggccgac
    11701 caggatttgt tttaccaggc tgccatgagc cagacgccgg aatagccccg gtgaacgttg
    11761 cgtagtcatt tttcgatcct ttcattgtgt tgtcgcacat ccctggtgcg ttctgtcgac
    11821 gcatctttca ggatgtaaca aagtgtttgc aaggtcgagt ataaggaaag ttacagcttc
    11881 gggaagcgaa aaatgctgga ttttaaagag aggtcatatt ttgcaactta attgttacat
    11941 ttgtttgaca taaacagagg gaaggactgg ctactaaggc tgattagata acatatgggc
    12001 aaaactattt ggaatatgag aacggttgcg ctcctttaat gtgttatgcc atataaagtc
    12061 actggcgcag ggagcgcgca gggggcggcc aatcgccgcc gccccctgca cccccaggct
    12121 ctggcgaaca aaatcgccgc tgcgcggttc cctcagctta tcccttgcgg ctaccgggtc
    12181 gggcgcaagg taacgtccct gtaagatgcg ccctcgccgc gcatccatgc gcgtcgcccc
    12241 ggcctccagg gaacgcttcg gcgattttga cgccaccaac acccgtgcgg tctttttgta
    12301 aagagctata agccgattac ataattaata gctcaccttt gtctggaatg agttatctct
    12361 agtaatggct taatatacag cggatcattc tccccgctgc ttatcatgcc gataattcac
    12421 ccaggcgttg ataataaacg tcatcaccag aatgccaaac accacgccca gcgagacggc
    12481 gattggaata tggtagaagt cgacaatcag catcttgata ccgataaaca ccagaatcac
    12541 cgccaggcca tatttgagca tcgagaaacg ctctgctacg cccgccagca ggaaatacat
    12601 cgcacgcagg ccgaggatcg caaacaggtt tgaggtcagc acaatgaacg ggtcagtggt
    12661 cacggcgaag atagccggaa tgctatccac ggcgaaaatc acgtcgctca attccaccag
    12721 aatcagtacc agcatcagcg gtgtggcgta cagcaatcca ttcttacgca caaagaaatg
    12781 ctcgttgtcg atggtgtcgg tcatgcgcaa atgaccgcgt agccagcgca ccagcggctt
    12841 gtcgccaatg cctgattcat cttcatgggc gagcgccatc ttcacgccgg taaacagcag
    12901 gaaggcaccg aagatataca ggatccagtc gaactgcgaa attagccagc tgccagtgaa
    12961 gatcatgatg gtacgcagaa caatcgcccc gagcacgcca tacaccagca cgcggcgttg
    13021 taatgccgcc ggaacagaga aatagctgaa caacatcagc cagacaaaga cgttatcgac
    13081 cgccagcgat ttctcaatca gataacctgt gagaaaggcc agtgcctgtg gatccgcaac
    13141 ggcgcgacct tcggtttgca ccagatacca ccagaaagcg gcgttaaaca gtaacgacag
    13201 cgtcacccag accagcgacc aggccgcagc ctgtttcatg gtcatggcat gtgccccacg
    13261 acgcccctgc aacaacaggt cgatagccag cataatggcg acaacaacag cgaatccgcc
    13321 ccatagcaac ggcgtgccga cagtattcat agaagttcct tacacataaa aaaacggcca
    13381 acgtcggaag acgtcagccg ctgcttttta tgcatagacc tcgccttccg gcaaggtctc
    13441 acttacaaca aaaaaggaat acgtctgcgt attcctttcg ggttgcccgg tgaccggatg
    13501 tggtttttca cacatcgtaa tgacgatcga ccggcaatga agttactccc ctttgcaggt
    13561 aacaaagtat gacagaccat tcagccagtc aatggtctgt tacatacctt ttacactgtt
    13621 ttactttggc aattatagtt ttacgctatc tgccggaaaa atcaccccag tctggcggcg
    13681 gatctcggtc agcagtttgg cggtgatgcg actgaccgcc agccccggat gatccaccag
    13741 atgctcatcc accagggtag cgaacagctc tgcttcgtag agcatggtat taatatgctg
    13801 cggctgggtg agatcctgca tttggctgcc acgcggcacg aagcacactt tctggcattc
    13861 agacagtttt tcaatcacca gcgatcctgc ttcgccctga atctcgctcg ccaggacaga
    13921 atcactgact tttgagtgct gcaaggtgac gctgaaatca ccgtaatcca tcaccaccac
    13981 gccgtgggca tctacaccgc ttgccagcaa actggcggtt gcctgcacgc ttttcggctc
    14041 accaaataac gccaccgccg acgccaggca gtaaaagccg atatccataa ttgaaccgtt
    14101 agagaatgac ggattaaagg tgttgggatt ttcaccgtcc aggtaacgtt gatagcgcga
    14161 ggaatactgg caatagttga aaaagacttt acgcaatttg ccgactttcg gcagcgcctg
    14221 gcgcagcaaa tgaaagttcg gcaggcaggc ggttttaaat gcctcaaaca gcaccacctg
    14281 attttcccgc gcacaggcaa tggcggcatc cacttccgcc agattcgacg ccagtggttt
    14341 ctcgcaaatc acatgaattt tatggctaag gaaaagttgt gtctgggaaa agtgcagggc
    14401 attcgggctg gcaatataca ccgcgtcaat ggcatcgctt tccgccatcg cttccagcga
    14461 ggtaaacaga tgctcgacag aaaaatcatt ggcgaagtgc tgagcctgct caaggctgcg
    14521 ggaatatacg gcggttaact tgtatttacc gctctcatgg gcggcctcga cgaactggcg
    14581 agtgatccag ttcgtaccaa tcacagcgaa acgtatcata acgcgtggat actccataaa
    14641 gggattctgt cgtcaatgta gcacgccttt ttcatcttcg acgtgcgcag atacgcatta
    14701 ttcttttgcc ggacagccat gctcactaaa cggcccaatc atgaccgatg gcccgagata
    14761 tttcggctgg cgatggagga ttttggcgtc aatcacggcg ctgacacgag cgagtttctc
    14821 ccgcagttga gtataaaaac cgtgcttcat attgaggtca ggcgcgttaa aaccgatagc
    14881 gtcgatgctg tactgttttg ccagccagat ggcgcgttgg ttatgaaatt cttgcgagat
    14941 aatggtgata tggttttcac caaaaacctt tttggcccgc actaccgaat ccagcgttga
    15001 gaatccggca tagtcgcaga agatcacttt cgccggtacg cctttagcga tcaaagcctt
    15061 ctgcatacct gatgcttcat cgtaattttt acgtccgtta tcgccactta ccagtagcca
    15121 tttcactttt ccggcgtggt acaacgctgc tgcggtatca atacgtcggg taaagtagcg
    15181 attacccggc ctggccccca gcaataagcc cacgttacgc tccggaactg cgttgacatc
    15241 gctccaggtc agctgcttgc tggcgttgac cataacgcga tcggcaataa aaatggtcgc
    15301 tcctgctata agcaacaaca aacagccaat cttaagagtc cggcgagaaa aacaaatacg
    15361 gagaagaagg cggggctgca tggtcgttat ccttaacgaa aggggggagg tcgattttag
    15421 gcgctaaaga aagaaaagat aacgcctgac atcattttca gattttgtcc tgcccaagcg
    15481 acaactgcgt tagccacagc cgggtgtcga actcaagctg gtggtactgc ggctccatat
    15541 ggcagcacaa ctggtaaaac gctttgttat gctctttctc cttcaggtgc gccagttcgt
    15601 gcaccacgat catacgtaaa aacggttccg gcgcgttgcg aaacacagta gcgacgcgga
    15661 tctctgcttt tgctttcagc ttgccgccct gcacacgaga aacagcggta tgtaacccga
    15721 gcgcgttttt tagcacatgg atcttattgt catacatcac tttattgatc ggcggggcat
    15781 tgcgcagaaa ctgatttttt agatcctgag tatattgcca gagggcttta tcggtggcgt
    15841 agtcgtgtgt tcccggatag cgttttgcca gcacatcgcc cagacgctgt tcgttaatca
    15901 gcgttcgcac ctgggaaagt agctgctcgg gataaccctg aagataagta agattgctca
    15961 taaccgccca cgaaaaagag aaaaagggta tactcacgca cccttttcag gggcaacgtc
    16021 gaaattttat cattcaggag ggccgatgag ccacttagac aacggtttcc gttcactgac
    16081 actacaacgt tttccggcga cggatgacgt taacccgcta caggcgtggg aagcggcgga
    16141 tgaatatttg ctgcaacagt tggacgacac agaaatccgc ggcccggtgt tgatcctgaa
    16201 tgatgccttt ggtgcgttaa gctgcgcgct ggcggaacat aagccgtaca gcattggcga
    16261 ctcatacatc agtgaactgg cgacgcgcga gaatttacgc ctcaacggga ttgatgagtc
    16321 gagcgtgaag tttctcgata gcaccgccga ctacccgcaa cagccgggtg tggtgctgat
    16381 caaagtgccg aaaacactgg cattgctgga acagcaactg cgtgcgctgc gcaaagtggt
    16441 cacgtcggat acacgtatta ttgccggtgc caaagcccgt gacattcaca cttccacgct
    16501 ggagctgttc gagaaagtgc tcggcccgac caccactacg ctggcatgga aaaaagcgcg
    16561 cctgattaat tgcactttca atgaaccgcc gctggccgat gcgccgcaga ccgttagctg
    16621 gaagctggaa ggtactgact ggactatcca caaccatgcg aatgtcttct cccgcaccgg
    16681 gctggatatt ggcgcacgct tctttatgca acatctgcca gagaatctcg aaggtgagat
    16741 tgtcgatctc ggctgtggta acggcgttat tggcctgacg ctgcttgata aaaacccgca
    16801 ggcgaaagtg gtgtttgtcg atgaatcgcc gatggcggtc gcttccagcc gaatgaatgt
    16861 tgaaaccaac atgccagagg cgttggatcg ctgcgagttt atgatcaaca acgcgctctc
    16921 cggagtggag cctttccgct ttaatgctgt gctctgcaac ccgccgtttc atcagcaaca
    16981 tgcgctgacc gataacgtcg cctgggagat gttccaccac gcccgccgct gcctgaaaat
    17041 caacggcgaa ctgtatatcg ttgccaaccg tcacctggat tacttccata aactgaagaa
    17101 gattttcggc aactgcacca ccatcgccac gaataataaa tttgtggtgc tgaaaacagt
    17161 gaagctgggg cgtcgtcggt aagattggga gttacctgcc ggatgcggcg ttaacgcctt
    17221 atccggccta cgttcagcac gttactccca ggccggataa gacgcggcaa gcgtcgcatc
    17281 cggcaatgtc tgcccagttc tggaatcgcg cttccataaa acaaaaaatc cctgtcacca
    17341 tcctctacat tctcttgttt agcgttgttc aacgtttatg ctacatacgt gaagttatga
    17401 cgcataaaga atacgatttc tttaccgctg ttcatcgtac taaggggaaa aaatgattgc
    17461 gattgccgac atcttgcaag caggagaaaa gctaactgcg gtggcacctt ttctggcggg
    17521 tattcagaac gaggaacaat acacccaggc gctggaactg gtagatcatc tgctgctcaa
    17581 cgatcctgaa aaccccttgc tggatctggt gtgtgccaaa ataaccgcgt gggaagaatc
    17641 agcgcccgaa tttgcggaat ttaatgccat ggctcaagcc atgtctggcg gtatagccgt
    17701 gattcgtacc cttatggatc aatatggttt aaccctttcc gatctgccgg aaattggcag
    17761 taaatctatg gtgtcacgcg ttttgagcgg gaagaggaaa ttaacgctgg aacacgctaa
    17821 aaaattggca acgcgattcg gcatttctcc cgccttgttt attgattaac gcgacgtgcc
    17881 ggatgcgttg catccggcgc ttcaaataac gatttaaatc tccagcgcca gccgtgttcc
    17941 ctgggcaatt gcccgtcgtg cgtccagctc catagccaca tcgcagccgc caattaaatg
    18001 cacagttttc ccgctatcaa tcagcggttg cgccagcgcg cggtttggct cttgccctgc
    18061 gcagatcacc acattgtcca ccgcgaatac ctgtgtttcg ccgttgatca ccacatgcag
    18121 cccgtcatcg tcgatcttct ggtaactgac gcctgggatc attttcacac cgcgcgagag
    18181 cagggtagtg cgatggatcc agccggtggt tttgcctaac ccctgtcccg gtttgctggc
    18241 tttgcgctgg agcatcacaa tttgccgtgg gctacgcggg atctgcattc cctgcgggct
    18301 taagccacca gcctgttgta ggctgctgtc gatcccccac tcattacaga acccggcgat
    18361 attctggctg gtggattcgc ccggctgact taaatacatc gccgtatcaa aaccaatccc
    18421 gccacaaccg atgatggcaa ccttgttgcc taccggcgct ttgtcgcgca gtacatcgag
    18481 ataactcaat accttcggat gatcgatccc gtcgataggc ggaacgcgcg gcacgatccc
    18541 actggcgagg atcgtttcat cgaacgcctg taactgatcc gccgtcacgg tgtgattgag
    18601 tttcagcgtc acgcccgtca cttcgatcat ccggtggtaa tagcgcagcg tttcgtaaaa
    18661 ctcctctttg ccggggatct gtttggcgat attaaactgc ccgccgatct cgctgtgggc
    18721 atcaaacaat gttacctgat gcccacgcgc cgccgcgtta atggcaaacg ccagcccagc
    18781 aggtcccgca ccgaccaccg ccagattttt tttctgcacg gcaggaagga ttggcatttt
    18841 ggtttcgtgg caggcgcgag gattcaccag gcacgaggtg actttgccaa cgaagatctg
    18901 atcgaggcag gcctgattgc agccaatgca ggtgttgatc tcatcggctc gccccgattg
    18961 cgcttttgac agcagttccg catcagcaag aaacggtcgt gccatcgaca ccatatcggc
    19021 atcgccgcgc gagagaatgt cgtcggcaac ctgcggatcg ttaatccggt tggtggtcac
    19081 cagcggcagc gaaacgtggc ctttcagttt gcgcgtgacc caagtaaatg cgccgcgcgg
    19141 cacgggcgtg gcaatggtcg gaatacgtgc ttcatgccag ccaatgccgg tgttgataat
    19201 ggtcgcgccc gccgcttcaa tggcctgcgc cagctctacc gtttcggcaa aagtcccgcc
    19261 gtcttcgacc aggtcgagca tcgacagtcg gtagataata atgaagtcgt tgccgacgcg
    19321 ttcgcgcacc gcacgcacta cttctacggc aaatcgcatc cggttgcggt aatcgccgcc
    19381 ccactggtca ctacgctgat tggtgcgcag cgtcagaaat tcgttgatca aatacccttc
    19441 ggaccccatt acctctacac cgtcgtatcc tgcctcccgt gccagttgcg cgcagtgggc
    19501 gaaatcgtcg atcagttgca ggatctcttc atgggtgagt tcgtgaggaa cgaaacggtt
    19561 gatgggggcc tgcaatgcgg agggagcgac cagatgcggt tggtagctgt agcgcccggt
    19621 atgcaaaatt tgcagggcta ttttgccgcc ttcctgatgt accgcttcgg taatggtgcg
    19681 atggtgtggg atctggctgg cgtcgttgag cattgcgcca ccttccatgc caacgcctgt
    19741 taaatctggt gcaatgccgc cgctgacaat cagcgccacg ccgtgacggg cgcgttcggc
    19801 ataaaacgct gccagccgct cggcaccgtc cgggtattcc tccagcccgg tgtgcattga
    19861 gcccatcaac acgcggtttt ttaacgtggt aaaacctaaa tccagcgggg cgaacagcga
    19921 cgggtagctc ataaaattgt ccagtatgta aaataattgt tatgtggtcg gatgagttct
    19981 aatttagccg tcgccggaga aaagggaaaa ggggagtgcg gggtttgtga tgggattcaa
    20041 aaagaatacg cccggtggta acgccggatg cgacgcttgc cgcgttttat ccggcctggg
    20101 ggattgcaca tgtagggcgg ataaggcgtt cacgccgcat ccgccagtgg cacggtgcag
    20161 atgccggatg cgacgcttgt cgcgttttat ccggccagga ggattgcaca tgtagggcgg
    20221 ataaggcgtt tacgccgcat ccgccagcag ttgcaaatta ttgcttacgg aaaaaatcgt
    20281 tatacaacat atacaaatgc gcggcactcc aggagaaatt tggtgcgcct tgctgtgcgc
    20341 cagtcagcgg gttgtaattc tcctgaattg ggccatcggc ggttaatcct ttggcgtgac
    20401 ggaagaacgt atccgccagt ttcagggcat catcgcgata accgtaacgc tccatccctt
    20461 tcagaccaaa ccagaactga tccacccata cgcgcccacg ccagtagata tcagcgccaa
    20521 aagccggatt ggttaacgcc gccgttccca gcgggacaaa ggtgttgaac tctttaggat
    20581 cgagcatcac cttcaccacc gcatcggcat tggcctgcgt tgccgcaccg ttaaacagcg
    20641 gcgaccagcc ttccggccct ttaccgcgct caacaatcgg tttgcccgcg cagccgttcg
    20701 ccagtggttt atcttcaata cgtacgtcat agtagaactg ggtagtcggg tcgaacatac
    20761 aggtgttgat gtagtccgcg agctgctgtg ccaactggcg atagcgtttg gcctcttctg
    20821 gcttaccgag gatggttgcc atctccgcca gataatggtt atcgctgtac atatagctgg
    20881 cctgatccac cgactcctgc aatagcgagt agcccagcaa cgttccgtcc tgactgcggt
    20941 tttcggcgaa tttcaccgtc cagtcgctac gtttgccgcc attagcgaca tatttatcca
    21001 actgttcttt gtcgataaaa ccaaagacgg cggcatcatc acgccccgat tcccacgacg
    21061 cagcgacctg tgccggaatt tccagactgt cgtactggcc tttctccacc acgcgggcgt
    21121 agttgttcag cccagactgc gtctcttctt tgttgccttt cttcaccgta aacagcatct
    21181 cgccgctctc agtgttgtgg gctttgtcgc gggtcgcgcc atattccggc acgccgttgc
    21241 cgttgtgatc gcggttacgt aaccaccagt cgtgataggc caccagtttc gggtacatct
    21301 ctgccaacca ggctttatcc tgggtgacgt tgtacacttc catcaccgac caggcggcaa
    21361 ggctgggttt ggtattacgt tcgttccagt tgccgccatc gccaccacgc tcggggctaa
    21421 ggttccacgc tatcaggtcg gggacaaagc ccacatcctg cggacgcacg ctatcgccag
    21481 gctggatctg ccaggagaag accgcgcgga tattctcttt ggcgatgtcc ggattgaaat
    21541 gcgccatcgc aaacgcctgc ttccaggtat cccacggcca ggtctgattg ccggagaacc
    21601 agcgcccggt caccgacggt gtaacggtgt taaatttcac cgcaccgccc ggtgagcgcc
    21661 agttaccgtt gagcgtttcg atcgctttca ccgcgacgcg cgtctgttcc ggcgtcgcat
    21721 ccggattggt taaccctttc ttcaggtatt cttcccagcg ttgctgcgag gcggtgagat
    21781 aaaacgccgg acgcgccaga atatcgcgga tctgcatttg ctctttgcta acttcctgag
    21841 cggtcagcag atgggaatag gtggtgtaga gcgtggtcga accgttgata tgcgccttac
    21901 tggtaaagcg attaccgttg atttcagtct gcaccggcag ggatttatgc acctgatatt
    21961 ccgattcgcc ggaggtcagc agatcccagg tggcgcgcac tttgccaaag gtgactttca
    22021 ggccatcacg ggtggcgctg attttgcgct gatagtcagg gtattcgcca gcaatggttt
    22081 tatcggaaag cggtttccct tctttcgctt ccagtttttc cagcagttcg ccgtcccaca
    22141 ccagatccag cggtttattg ctggtgattt tggtttccag tagtgacgtg cgcggcgtgg
    22201 cgaagcgcag agtcatttcg acttgcacat cttttgctgt cagtttttgc accagcgcac
    22261 cgggaatact gtatgcctcc agcgtaaagt cgactttctt gccgtcctgc cagacggtca
    22321 ggcggtcgaa attgctggcc ataaagttga tgtactcttc cgtcagcagc gcaacgcccg
    22381 gaaagccgcc catggtgtta gggccgtctg gcaacagatg accatgccag gctccgagat
    22441 cgaaaaacgg attaaaacgc tggtgatcgt cgtaatcgta atccttcatg tactgcggcg
    22501 cgccagtacg gttaatcacg tttttataat tgtcggcgtt agcggcatgg gcggaaaaac
    22561 ttatcagcag agcgcaggtt actggcgtta aaatagtttt tattttcatg aaattacagt
    22621 ccttattacc agtagaaata ttgcatcagg aagatttggt cgctgggatc gtgagaggca
    22681 tttttcatgt agaagcggct atcaaaagcc gtggcgaaac gtccgccgcc gtattcgtac
    22741 cagataccaa actgcatgaa agaggtggtt tcatcgtcag cgtggtcagg gcgatgtttg
    22801 gcgtaggtgt aagcggtgga gagataaacg ccctttaacg cttcatacat ggcgctcacc
    22861 atcatcccac tttcacttcc cggtacgtcc agcccgtcgg cgcgtccggt atggtgccag
    22921 gcgcgagcgg caaagttagg agttagcagg cccgtgagat agagctgtcc tgtgccgtcg
    22981 gtgatttcca gaccgttcat ccaggtgatg ccttcggcaa ggtcatactg gatgtaaccg
    23041 ttcatcattg ccggataggt gtatttgtcg tcatagcgat cgtacttgcc aaaatgtagc
    23101 caggctttgc tttcgtcatg atgtccggca ggtgtggcgg tgacgctata acgaaagttg
    23161 ccgcttaagt tctgcacctt cagcgcggtc ataatgtcgc gggtattggg aatgacgtag
    23221 cccagatcgg gggtgaagtc gccccaccat tgcaggtcat ccagcgagga atcctgacgc
    23281 agactgagca tgatttcggt gccatcattg gtacgataac cgccatagaa acggttaatt
    23341 ccgccctcga aaccacccca gctatggtct ggcacccagg cattgccgtc gtggtcggcc
    23401 tgcacggtcc agccttcgcc gtaaatcagg ccgaaccagg agccgtactt catctccagg
    23461 ccaccttcaa tataggtacc gtcgctgtat ttatgtttat tctcaccctc taaatccata
    23521 tatccaccaa cccctaattc gccgtaaaaa cgaaatgaag gagaagatgt cgaggtaatt
    23581 gcaggtgttt ttgtgtcgtc atgttctgcc ggacgtgctt cttctcctgc cgcagaaaag
    23641 gcgtagcaaa acggcagagc aagtaatgct ctgcatggtg cagtgattaa tttcattgtg
    23701 ttaatccatg agctaaagaa aaagtacagc cttttatcgt tgtaattata aaaatataaa
    23761 ggcagaaact attttccctc tcctcatcca ggaagaggga aaatgcctgc tcatttttta
    23821 ttattctgct taatcaacat tgctggctgg cgtcgcttca atatggcgct cttcagccgt
    23881 taatcccttt atatatttac tgtatttcca ccacgcaaag ccaaggaaga taacaatacc
    23941 gccgacgtta taaaaaatga tggtcagaat attcgcgcca gtcgggaatg tcgaagcgac
    24001 aaaccctacg gcaaatatcg caatcagcat tgaaacaacg ataataccgg tgcggcggga
    24061 gcccatacgg aaatcgcgtg gcaggtgatc taatttggcg cgtaaattca ggtaagccag
    24121 catgatgaat aacggcggaa gcatggacgc tgcggcggtc atattaataa tagtattcat
    24181 cagatcctgc acggtattgg aaccgagcat cgggataatc atcagcggga tgacgatcag
    24241 gaactggatc cacgctgcgc gcgccggaac gccgttttcg ttcagttcga cggttttctt
    24301 accaaagatg ccttccggga tttcggagaa gaaaattttc accggcgttg cggtccacat
    24361 cagcagggaa ccgaacatcg cggtaaagga caccagaccg acaaagcgat tcatcaacgc
    24421 ttccggtaga ccaaaatacg ccgccatgcc gtggaatacc tgcaccgatc cgccggtaaa
    24481 tttcaactct ttgctgctga cgaagacgtt aatcagcacc gaggagacgg aatacagtac
    24541 gccgataaaa atcccggcga ggatgatcac tttaacgaac gatttcgaac cgcctttgac
    24601 gtcgttaacg tacacagcga cggactccgc gccacctgcg gccataaaga tccaggtggt
    24661 aacgccgagg aacgcccagt tgaagttcgg gatcatcgcg tcaacggtga tggggtcagc
    24721 aggctgtacg ccgccaacca gcgccgtacc tgccagtaaa atgtaggaga gcgttaacag
    24781 cagcatcagc gttgaagtga cggaggtaat tggccccaac attttcgccc cgttggtgga
    24841 aacccaggtg gagaaggcga acagcaccat actgataatg gtggtggcaa ccggcgtcat
    24901 aatatattcg tatccgagga aggcatacga agcataagca ataacgcgcg gcaacagtga
    24961 ggtgaaaaag aacaggttta cgaaccagta ggtataggca gtaataaatg cccaacgtcc
    25021 gcccagcgaa ctttttaccc acgcgtagac accggcttct gagtttttat ttaacgaaac
    25081 aaattctgcg atgatcagac agaagggaat aaaataaaaa atcgtcgcga ggaaaaacat
    25141 cggtgccgag gccagtccaa gctcaatatt attattgata acgttgttaa agctgtaaac
    25201 ggcggcaaaa gtcagcgaga gcaagccgaa tttgccgatt gtattacgtt tggtatcaga
    25261 cataacacat ctccgaaaat gtgtagggta gcggtggcgg caaactcctt ttgccgccgt
    25321 agttgttatt tgttatggaa ataaccatct tcgatggtga ctttgagaac cacttttttg
    25381 accgcgttat tgcagataaa ccgatacgct tcatggatat cgcaaataac gatttgccct
    25441 tcgtggacct caacggtttc tccgcagcct tttaaatatt cacggtcagt ttcatcacga
    25501 taatattcca ctacctgtaa tgtctctttc ggcgcatatt caattttttg ctgcccttgc
    25561 aggtagtaat gcacttcaaa atagcgtcga tgcccggtaa atagcgcatc ggtcgcggag
    25621 tctgtctcca cgcggtaagt caatgagtca ccgatggagt gggcgacgcc aggctgaatg
    25681 ttgtcgatat tttcaatcgc ttcaacgcag cgttgccact tcttgccaga ggcgtaaatc
    25741 tggcggaact gttctaagtt atcgatgatc ctcattgctt attctcgctg tgcaaattcg
    25801 tggaaaagaa ccctgcgccg aactcatacg acgccaggct ttgcgcggta gcttctccgc
    25861 cagaaaccgg cagcaacgta aagccgtagc tgaagtcacg gaaccagacg cgccaggagt
    25921 ccagcacctc gctgccccag gagttggagc cgaggccaag cagctggtgg tcgaggttca
    25981 gggtgatgtc atcactgcgc tgcagctcgt tacagtgctg ggcagcgtgg atgttttcct
    26041 gggtatagcg ccaggcgctg aagttaattg ggcgctgcgg aaccaccagc agaccgttgc
    26101 cgtggcggtt agtcagtgcc gtccagcgga catgctgacg gttgccgttg ttctgcggga
    26161 agggatagtt ctcgaacatg gcatcgacgg tgctgcgcca gatatcgatg atgttagcct
    26221 gctggctgtc ggcgtagttt tcgcccggtc cacgaccgta atacgccacc tgatcgtatt
    26281 cgccgttaat ccccatggtg aaaccgatgc acggaatgat gtgcggatag tcgccgtaac
    26341 gctcgccgga aagcgccacg ttaacctggc catcggcagc gatgcgccag atgtaggtgc
    26401 agcgcatccc gaagtcaaac accggcgggg caataactgt gcggctgatg atcaacactt
    26461 caccatcgct ctgttctacg gcaaagtcgc gcagatgttc ctgcatgatc tgcaaatgat
    26521 tcggttgcca cagcccttcg tactcctgct tgtggttgtc gatcattggc ttgaagaagt
    26581 tgatctttgg ctcgcgggtc agcagcgact cgccattcac ctgccaggat gtcggtttgc
    26641 cactcatttt tgagaaggtg atcgcgaagt tgtagccgcg aacggtgcag ctcaaacgat
    26701 cgtcttccag cgtcagcgga cgcgcattat ttggtgcgaa aggcactggt tgcgcggtgt
    26761 tttccttcag cgggaactga taagtggcga tagaatgccc ggcttcgctg tagcgggtgc
    26821 gggaatcttt ggtcaccgta atgttgagga acgcttcgcg ggcgtccagc tgcggcagcg
    26881 tgatctgcaa gggggcttcg ctgttcggcg caacgtcgcg cagtttaatc tgctgcgtcg
    26941 cgagcgtttc accttcgacg cgcacctctg cgtgcagggt gtagtcatca agcgtggtaa
    27001 accacagttt attttcgact ttcagctcgc cgtgagtcag atccagcgcg tggattttta
    27061 ccggcgcgat aacctgtttg tactctttca ggcccggtcc cggcgtctga tcggaataga
    27121 tcaaaccatc aagacagaag ttatagttgt tgggatagtc gccgtagtcg ccgccgaatt
    27181 tataccagac gttgccgttg tcatcctgcg cctggatccc gtggtcgcac cattcccaga
    27241 cataatgtcc ctgaatgcaa tcgtgcttat agaagacgtt ctggtactcc gtcagcccgc
    27301 ctggtccgtt ccccatcgca tgagcatatt cacagatgat gcgcggcttc ggatgcgggt
    27361 attcaccaaa ctcattcatc agcggcacgc gggtgtacat ggtggaaata atatcgacca
    27421 cttcagcatc gcgatcttct tcgtaatgca ccagtcgcgt gtcatccagc gccttcgctg
    27481 cgtggtacat cgcgcggatg ttacagccat agccggattc attgcccagc gaccagatga
    27541 tgatcgacgg atggtttttc tgcgcgtgaa tatggcgaac aatgcgctcg acgtagactt
    27601 tttcccactg cggatcgtcg gtaatacggc tgatatcgcc gacattagca aagccgtgcg
    27661 attcgacgtc ggtttccgcc atcacaaaca agccgtagat atcacacagt tcgtaaaaac
    27721 gcggatcgtt cgggtagtga gcggtacgca cggagttgat attgtgctgc ttcatcaact
    27781 ggagatcttt ctcgacgcga tccattccaa cggcgcggcc tttgcgatga tcgttgtcgt
    27841 gacggttgac gccgtgcagc atcacataac ggttattgat ccagaacaga ccgtcgcgca
    27901 ctttgatatc acggaagcca acgcgttgtg gcaccacttc cagaacgttg ccgttggcgt
    27961 ctttcagcgt catgaccaga tggtaaagat aaggggattc tgctgaccat tgctgcggct
    28021 gttcgacagt aaaagcaaag ctggcgctgg tcagtttttc aattgccaaa tgatcaatgg
    28081 cgctgctgtg caccacgcgt tcgccatcaa acagggtata ttccagcgtc gtgacgacag
    28141 gggaggcggc gagattttcc agcaccactt cgcaggaaag cgtggcatcg caataggctt
    28201 cgtcaaagtc ggtacgcaca gtgaaatcgt taatatgcgt taggtgtttt ccgaccagat
    28261 aaacatcgcg gaagatcccc gctgaccacc acatatcctg gtcttccacg taggtagagt
    28321 ccgcccactg catcacgcgc acacacaaca ggttgtcgcc ggttttaacc atcgcgctga
    28381 tgtcaaactc tgcggtcagg cgactgccct tgctgaaacc cacatactga ccgttaacat
    28441 agacttcaaa ataggtttcg acgccgtcaa atttaatcag cgtctgttta ccctgccagc
    28501 cgtcgctgag ggtgaaaata cgttgatagg caccggttgg gttatcgctg gggacaaacg
    28561 gcacatcgat ggggaacgga aaaccttcgt cggtatattg cagtttgccg tgaccttcca
    28621 tttgccacat ggcggggacg gtaatatgcc cccagtcagc cattaactca gaggtgaagg
    28681 cttctggtac ttgcagcgga tggtcaaaaa agtggaaatt ccactgaccg cttaagggca
    28741 gaaacaggct gctggtttcg cgggcaaagg tacgcgcttg cgcaacagaa tcatatgaaa
    28801 aaaagtacgc acgcggcgca agtcggtttt cgtgggtgag ctgaatgttt tcccagcgat
    28861 tcatagggcc tcccggtaga gagaaatcgg aaccattcat ggtgatggta gtttgtgtaa
    28921 ttgctgggga gtttagtaaa atttttacta aaaagtagca ggtaaatgca tttactttaa
    28981 ctggatcacg aaaataaacg gcagcactgt ccggcaaaaa cgattgccgg acagtgtgta
    29041 aggggattta acgggtcgtg ccgcgcagtt ttaatttgct gggaacgaag actaacagcg
    29101 gcagcgcgcg accatcgcgg gctttttcat acaccaggtt aacgccctga cttcccatca
    29161 tctcggaatg gatgcgcacg gtggagagcg gcggaaaggt aaatcgcgcg gtggggatat
    29221 cgttaacgct gataagcgaa atatcctgtg ggatgttcag gcctcgttca tgaattgccc
    29281 gcagtacgcc gatagcaatg gaatcggaag caacaaacag tgccttcgga tagtcttctc
    29341 gcgccagcat ttgttttgcc agttcataac ccgacgaact ggaaaaaccg ccgcgccaga
    29401 tatcctcttc gcgtaccact tgtttcagtc ggccatattc cgcaaaggcg acctcacgaa
    29461 tatccgcctt gccaggctca tcttcaccgc caataaaacc aatacgatta acgccctggt
    29521 tgatatagaa gtcgatgatt tctttactga tgcgtgccag atcgatatcc accgcatcgt
    29581 aaccgctgcc gggttcgtga aagtcgataa aacagatatt gtcggtcaac gcacaggcgg
    29641 cggcgcgcag ggcgggcgtg ggtttgccga caattaaaat accggtgacg tttttaatgt
    29701 ctggtaatcc gctgtgttca taacagttgg tgagctcaat gcccagcttt tcgcactgag
    29761 tttcaatgcc gtggcggatc gccagatagt aaggatcgtt gatctccagc tcctgttggt
    29821 agctgtagat agccagaata tggtgttggt tgactgcacc tgtctggagt ttacgggcac
    29881 tactggtctt gtactccagc ttttcggcga tctcgagaat gcgatgtttc gtctcttctt
    29941 tcacattcaa tgtcggatcg tcatttaaga ccctggatac tgtcgccagg gatacgccag
    30001 cttcgattgc gatgtctttt agtgttgcca tgtttacctt ttttactttc cctgatgtgc
    30061 gcccccattg tagaggaagt gcttatcggg tcacctgttt tcagtaaata aagcgcgttt
    30121 ctttgacgct gtaacggtaa cacagcggct cacgaatgtg atcacgcctg cattctccat
    30181 ttttagccta caggattgag aaaaactttt gctatagttt agtaaaattt tacccgaagg
    30241 gaggaaacaa tggaaaccgt ggcttacgct gattttgcac gtctggaaat gcgcgtcgga
    30301 aagattgtgg aagtgaaacg ccatgaaaac gccgacaagc tgtacatcgt acaggttgat
    30361 gtgggggaaa aaacactgca aaccgtgacc agcctggtgc cgtactacag cgaagaagag
    30421 ctgatgggga aaacggtggt ggtcttatgc aatctgcaaa aagcgaaaat gcgtggtgaa
    30481 acgtcggaat gcatgctgtt gtgtgcggaa accgatgacg gcagtgaaag cgtgttgtta
    30541 acgccggagc ggatgatgcc tgcgggcgta cgcgtcgtat aaaaagatcg gatggcgacg
    30601 tcgtatcgcc atccgatttg atattacgct tcttcgacac ttactcgcat ggccgccagc
    30661 gccttacgcg ccgctttgat caccagttca cactgttcaa tggtcagtgt cagtggcggt
    30721 tcaatgcgga tcgttttggc gttattgagc gttccggcca ccagtacgcg ctggcggaac
    30781 atctcgctgg caaagttata gccgatttcg ttatcaacaa actcaattgc catcaacatc
    30841 cctttaccac gcgcttcctg taccagatcg ggatattccc gcgccagttg acggaaaccg
    30901 tccagcaaca tatcgccttt ttgctcagcc tgagccggta agttctgctc cagcaacaca
    30961 ttgatggtcg ccagcgccgc cgcacaggcc agcgggttgc cgccaaaggt ggtggtatgc
    31021 aggaatgggt tgtcgaacag aactgaaaac acctcttcag tggcgatggt cgcgccaatc
    31081 ggcatcacgc cgccgccgag cgctttggca aggcagagga tatccggctg tacgttctca
    31141 tgctcgcagg cgaacatctt gcccgtgcgc cccatgcccg tttgtacttc atcgaggatc
    31201 atcagtgcgc cgaactcatc gcatagctta cgtacggcgg tgagatagcc cggcggcggc
    31261 agaattacgc caccttcacc ctgaatcggt tcgaggatca ccgcagccac atcatcaccg
    31321 gtttttttgc actcgttaag agccgtgcgc atggcttcga tattgccaaa cggcacatga
    31381 cggaagcccg gcagcagtgg cataaacggt ttgcggaagg tcgatttcgc cgtggccgac
    31441 agcgcgccaa gtgatttacc gtggaacgcg ccgctggtgg caataaaagt aaacttgccg
    31501 cgcggtgact ggtaagcttt cgccagcttc agcgctgctt caacggactc ggtgccgcta
    31561 ttacagaaga agctgtattt cagtttaccg ggcgttagcg cagcaagggt tttcgccaac
    31621 atcgcccgta acggatcgag cagctcctgg ctgtgcagcg gttgtttcgc aagttgattc
    31681 tgtacggcgg aaaccacaac tggattacgg tgccccacgt tgaaaattcc aaaacctccc
    31741 aggcagtcga taaactcctg tccctgggtg tcgacaagcg tatttaaacc tcccgcttgc
    31801 cactctacgg ctccgtaatc cccgccggcg gtaacagatt tgcgatactc taaaaacccc
    31861 ggattgacat gctctttgaa gtattcaatc acctctcggt taagtgcttt catctcctca
    31921 tgatccagcg ttcgcttctc aatgagattc agggcgtggg cgctgcacgc taaagccgat
    31981 gcgctcgaag gtaacctgtt caaaatatgc tccggaggct cgcgtatcac atgataccga
    32041 tttaagtatt gcagggattg cgccactccg gctgcgatcg cgaaaatcgg gataaacaca
    32101 aattcggtta tcatttgtgc aatatttaat gctatctgtt aacatttgtt gttattttgc
    32161 acggtttttg tcgggtatgg atttgatgat tgctgcgcaa atgcacgttt tcagggcaat
    32221 aattgcctga tttttgggca agataattaa cgttttcgta tagtaacaga gtgaaagtat
    32281 tttaaatcaa agaattagat ttgtgcttta atcgcaaatt gcgatctaaa tcaaattaat
    32341 cggttaaaga taaccgcagc ggggccgaca taaactctga caagaagtta acaaccatat
    32401 aacctgcaca ggacgcgaac atgtcttctc atccgtatgt cacccagcaa aataccccgc
    32461 tggcggacga taccactctg atgtccacta ccgatctgca aagctatatc actcatgcta
    32521 atgacacttt tgtgcaggtg agcggcttta ccttgcaaga gttacaaggg cagccgcaca
    32581 acatggtgcg tcacccggat atgccaaaag cggcgtttgc ggatatgtgg ttcaccctga
    32641 aaaaagggga gccctggagc ggcatcgtga aaaatcgccg caaaaatggt gaccattatt
    32701 gggtgcgggc caatgcggta ccgatggtgc gcgagggaaa aatcagtggc tatatgtcga
    32761 ttcgtacccg ggcgacggat gaagagattg cggcggtgga gccgctgtac aaagcgctga
    32821 acgccggacg taccagtaag cgtattcata aaggcctggt ggtgcgtaaa ggctggctgg
    32881 gtaaactgcc ttcattaccg cttcgctggc gggcgcgtgg agtgatgacc ctgatgttta
    32941 tcttgctggc ggccatgctt tggtttgttg ctgccccggt ggtgacgtat ttcctctgtg
    33001 tgttagtggt attgttggca agcgcttgtt ttgaatggca gattgtgcgc ccgatagaaa
    33061 atgtcgcccg tcaggcactg aaggtggcga ccggagagcg taatagtgtt gagcacctga
    33121 atcgcagcga tgagctgggg ctgacattac gcgcggtagg gcagcttggc ctgatgtgcc
    33181 gttggctaat taacgatgtc tcaagccagg tgtccagtgt cagaaatggc agtgagacgc
    33241 tggcgaaagg caccgatgaa ctgaacgaac atacccagca gacagttgat aacgttcagc
    33301 aaacggtggc gaccatgaac caaatggcgg cgtcggctgc cgataaactt tctatcaccg
    33361 ccagtaatgc ggcagtgcag ggtggggagg cgatgaccac ggtgatcaag acaatggacg
    33421 atatcgccga cagtacccag cgcattggca ccattacttc gctgattaac gatattgcgt
    33481 ttcagaccaa tattctggcc ctgaatgcgg cggtggaagc ggcgcgtgcc ggcgaacagg
    33541 gcaaaggttt tgcagtggtg gcaggggaag tgcgtcattt agccagccgc agcgctaatg
    33601 ctgccaacga tattcgcaag ctgattgatg ccagtgctga taaggtgcaa tccggttcgc
    33661 agcaggtaca cgccgccgga cggacgatgg aagatattgt ggcacaggtg aaaaacgtca
    33721 cccagttgat cgcccagatt agccattcaa cgctggaaca ggccgatggg ctttccagcc
    33781 tgacccgtgc agtggatgag cttaacctga tcacccagaa aaatgccgag ctggtggaag
    33841 agagtgcgca ggtgtcggag atggtgaaac accgcgccag ccgactggaa gacgcggtga
    33901 cggtgctgca ttaatcggta tgccggatcc ggcggttgga gcacaatgcc tgatgcgatg
    33961 ctggcgcatc ttatcaggcc tgcggggtgt gtagcgggtg taggccggat aaggcgcgtc
    34021 aagcgtcgca tccggcaatt gcaccgcgcc actggcggat gcggcgatta atccagttgc
    34081 gtaatatcaa aagcggcgcg gtcgatcacc gcaatgatct tttttatttg tgcatcactg
    34141 atatcgctct ggttgacgtg taaatccagc accgctttaa aattatccag cgcccgcttc
    34201 atttgcgggt tctggcgcag cgccgcgcca acgcaacgcg ctttgatgcg ttcttcaatc
    34261 atctccacct gttcgcggtt ttcttccagc cactgcgcgc cttgttcggt cagcgcaatc
    34321 tgcttcttac caccttcctc ttcgcggatg gtaatcagcg actgctcctg cagaaaatcc
    34381 agcgttgggt agatgacgcc cgggcttggg gtgtaattcc cctgggttag gttctcaatc
    34441 gctttaatca attcgtaacc gtggctgtca tcgcgcgaga gaatatccag aatcaccaga
    34501 cgcaattcac cgtgaccaaa gaaacgttgc cgacgaccgc cgccgcgacc gtggcgacca
    34561 ccgcagcatt gaccgtgttc atgctggtga ccgtgtccgc agtgctcgtg ttctgacttc
    34621 tcacctttgc agcagccctc atggcgtggc tggccttcat gtttacaaca cccttcgtga
    34681 tgatggctca tatcagcctc cttttgcttg attagatata tctaatttat atctgatgtg
    34741 agcattactg caagtacaaa aatgatgttt aaacgtggaa gcacctcaca tccaggccgg
    34801 ataagacgtt cacgccgcat ccggcagcat tgctatggca ctgaatgcct tcacatactt
    34861 cagatatatc agttctacaa atcgcttgca tttatcatga ttaacaatca ttatcattta
    34921 cgaaaattat ttagatatat ctgattacta tcacgaaggc gataacaatg aataactccc
    34981 cccgctaccc gcagcgcgtt cgcaatgatc tgcgcttccg tgaactgact gtgttacgcg
    35041 ttgagcgcat cagcgccggt tttcagcgca ttgtcctcgg cggcgaggcg ctggacggtt
    35101 ttacatcgcg cggctttgac gatcacagca aactcttctt tcctcaacct gacgctcact
    35161 ttgtgccgcc aacggtaacg gaagagggca tcgtctggcc ggaaggacca cgcccaccgt
    35221 cgcgtgacta tacgccactg tatgacgaac tacgccatga actggcgatc gatttcttta
    35281 ttcacgacgg tggggtcgcc agcggctggg cgatgcaggc gcaaccgggc gataaactta
    35341 cggtggcagg tccgcgcggt tcgctggtgg tgccggaaga ttacgcgtat cagctgtatg
    35401 tctgcgatga atccggaatg cccgcactgc gccgccgcct ggaaacgttg agcaaacttg
    35461 ccgttaaacc gcaagttagt gcgctggtta gcgtgcggga taacgcctgt caggattatc
    35521 tcgcgcacct tgatggtttt aatatcgaat ggctggcaca tgatgagcag gcggtagatg
    35581 cgcgtctggc gcagatgcaa atccctgccg atgattactt catctggata accggcgaag
    35641 gtaaagtcgt taagaattta agccgccgct ttgaagcgga acagtatgac ccacagcggg
    35701 tacgtgcagc ggcttactgg cacgcaaagt aactgatata taaaaagaaa aaggctgacg
    35761 atttctcgtc agcctttgct atatctgggc cctgctgtct ttgaactgaa tgcatatttt
    35821 gttgataaat aaagattatg tgtttttgat tttcgttttg tatgcctttt tgtatgcctt
    35881 ttttgaaaaa gatacccttg ttgtttgatc gtcgataacg ggtatcaatg tgcacatgca
    35941 agccgatggc gttcatgtgt gcgctgtcga acgtttgaaa atggttttag ggcggtttta
    36001 atgctggttg atgtggcgct ctgtggcgct ctgtggcgct cgttttggtg tgctggtggc
    36061 gggggtcgaa cccgcataca tgatcagcct gtacaggctg gcaccgactc tacccgttga
    36121 gcgtacacca gcaaaaaagc gatgaggttg ttggctgctg gtggtggggg tcgaacccac
    36181 ataaacatcc cgctttctgg aagctataca agcgggtgcc gctttaccga ttaagagtac
    36241 accatacagc caggcaaaat agtaatcatg cgttatgcgc gggtaaagtc tgtttcgtct
    36301 ggtagcacca gatgaacggg tatcattatt tcacctgatt tcacgtgatt tcgcttaatt
    36361 tcacaaaaat caccttcttt caccttcttt cacaaaaaaa acaaatcatc attaaagcgc
    36421 ctgattcgct ctctgatgcg ttttggtgtt ttgtggcgtc cgtgatgcca tgaaagggga
    36481 ttttttgcga tagcggcgca aatataacgg ttgctgctgg tatctgtggt tagtcttgcg
    36541 tgattttatt ggcgtgcttc tttctcagtc gtcgtatgat gtcgtaaata tgggtttcgg
    36601 atattgcgta ctctttgcgt aatgcctgca atttttcgcc gttatcccac ctgtagtaaa
    36661 tctcgtattc gcgcaatgct gcgaaaagtg atctgcctgt tggaaggtaa agacttcgcc
    36721 caccagcata gaaggcgatt gcagcgacaa cccgctttgc gattctgaaa gcgtctgcct
    36781 cctccatgcc attacgggta agctccgcta ctgaatgcga cacgatatca gcaagcgaat
    36841 cacaccatgc atcacctggt ttatctccgt tgtctatggc gtcgatgaat ttaagccgct
    36901 cgatgtcttt tggtacgtga tgggtttgct cgttcatccg gtagcctctt gcgttggtgt
    36961 atctggtcct accagtttaa tttaacgtcg caataaaacg atatacacgc acacaatcag
    37021 aaaggctaac cgactgttcc ggctctgtgc ttacttctgg tagctacgcc agattttttt
    37081 gcctctcccg tctatcccca tttctttttc agtgcgttta atcgcccgtt tcaggccgcc
    37141 taaatcatcc ggctgctccg gtggcctctg cgtcttcccg taacactctg ccctgcgcat
    37201 tgccacccgt tcgcgttccc tgtcagtctc caccagcatc atcacttcag cccatcgcgc
    37261 cgccgctctc cggtaaaagc ccttcgcctc cagttcttcc gctatgctgt catgtaccat
    37321 cgttcacccc tcagaacgga atattgtcac cgtaagggtc atcgcctccc gctggtggct
    37381 gattaccctg tgtgcctgtg gtcctgcgtc tgttgccacc tggtcgcgcc gttttcgcac
    37441 tgattacgct gtctgcaatg acctgataac cctgccgcgt ttccccgttc tgtccggtcc
    37501 actgactgac ctgcatcaca ccggatacgc tgattacgtt gcctttctca tgtcctgcca
    37561 gttggtcggc ctgcttacaa aatgccagga ctgatagcca taacgtcgcc tgtccgtcct
    37621 gtgcctgact gcatggcagc gataccgcca tacgtgccag cgtcatcggt gtgcccttac
    37681 tggtctgttt tgtctgcggg tcgtcaatca gtcgcccgta agctgctatc tgcgctgtca
    37741 tgattccacc tctccggttt taacgttgat ggttgttacc tgttccgctt ctgcaatctc
    37801 ccgctctgtc agcgtggtaa agtttgttgc tgctgtgctc atgaatgcac ttatcagcca
    37861 gggggctttc gcgtgtcttt ctgccgtgtt gtggcccttc gttcatgagc aaaacactta
    37921 aataaaaata aagtgatttt ctgtgttttc gtgctgctct gctctgtgtt ttttgctgtt
    37981 tactcaccac tactcacttt tttactcact ttttggaatc gctcacttcg tctcacctgt
    38041 gattttcggg ttttctatgt gcgtgtttta tatgtgattt tttactactc acttttttgc
    38101 ctatatacag gtgagaaagt gagtaatcgt gttaatattt tgtaaaaatc tcaccattac
    38161 tcacttttct actcactttt ttacagtggg cctacatcat caccgtcgat atagataacg
    38221 tcgtctttct ccagttttgc taaccatcgc cgcatgtttt tcacgtcata cccaagccgt
    38281 ttcatgtcat cacgtaacag cgggatcgtg cacttgtcgc cgttctgtgt gcgtgaacgt
    38341 atgcaccccc atagcgcggt atggttttcc gtcttgttcc ctgcctcctc gatgcgctca
    38401 ggttcaacgg gatgccgcgg cttatccacc accaccagcg acgtaattaa ctccccgtca
    38461 gcgtcggtaa aaagctccac cacgcgtaag tcatatgcgg cttctttgag ttcctccgcg
    38521 tccttcattt tggtacatga gataaccagc gcttcgcttc ctgcgtcctc cctgcgtatc
    38581 cggtattcag catccagcga agcacgaaat gcactggaac cgcgcgcgcc tttcgtctca
    38641 tccttgccgg aatggtgaac caccagcacc gtggcccctg tgcgccgttt aagctcgtca
    38701 cacccacgga taaatgcccc catatcacgg gaatcatttt catcattccc accaaagcaa
    38761 cgcgccagcg tatccagaat aatcatgcgt acaggtttac ccgtttcccg ttccacctgg
    38821 cgggcagcga taaccatttc atcaacatca agcggggcag ccggaaagat ggggcggttt
    38881 accagataca gatttttcac ctgctcaccg tgcacaacct cccaggcttt tacacgacgc
    38941 ggaacgccga taccgccttc accaaccaca tagagaacag cgccatgcgc caccctgcgg
    39001 cctccccact ggcggcccgt ggaaacgtga cacgcccacg aacccgcaag gaacgattta
    39061 taggaaccgc tcgccccgta tatgctgcac agcgatactg ccggaataat cccctttacc
    39121 acgtaatcaa gttgtgtatc gtatccggca gaaccaacgc tcatcggtag ctttgttttg
    39181 cgctggtggc ctttttcttc ctgcttttcc ccgcgctccc gttccagata ttcgcgccag
    39241 ttctcccgct tctggctgtg catcccatcg gggtaataat tcgcatcctg tacgcctgcc
    39301 gctgccagtt tttgcgcgat ggcattaata tttgatggtc tgatatggcc tgctttgtac
    39361 agccggacgc aatagcgccc ctcgtcaatg attttcgtgt ctgccagttc tgccagttgc
    39421 tgctccccaa gccatacagg tggcacatta tcgccagcaa gtcggccctc ctgctcctgc
    39481 cactgcttcg cgtgtgccca ggcatcacta cccgcaaaaa taatgacctc ggtcattttg
    39541 tcgtaaggct gtttttttaa gttcggtgcg cttttcattt cttgcccctg aatgcgttaa
    39601 tcatcttttt catcttctgt atttttgcca gtgcctttac gttgctggtg ggcacgttac
    39661 gcggtaccgc ctgcaccaga gaaaaatcac gggaaaactg ataaacaggc atcacgcagt
    39721 catattcgta gcctttacgg cggtaggtga cgcaccgccc cgccacgccc ttaatcatta
    39781 ccgtgccgcc gtactggtcg cggtaaatat caccgcgctt aaattcaggg cgaggcttgc
    39841 cgctggcagt taagccagtt tttttcatgt tcatttttta tcctttgctc tggtcagtta
    39901 cgccgttgct gcataaatgc ggttagcggt gcctgtcgtt gccttatcac gaatggcgat
    39961 aatcagcact gatatctccg ttacgacaac gccagttgta cacctccaca acctcactga
    40021 atccggccac aagttcagcc gtaacagcga tggaatcgcc caggctgcgc agttcgtcca
    40081 tgtcaggatc aatgctgtca ctcatgccag aaagtaagcg ccctgtgata gccagccctt
    40141 caagcaattt gcgggcgtag aagtcagcat catcaaacat cgctctcgct tcccgtgcgt
    40201 cagctgcttt tatggttgta accagttgtt caagtattct ggtgttacac acatcacgca
    40261 tgacgtaccc cctgacggat acgggcgaca aaaacaagat gtgcgtgcgg cagctgggcg
    40321 cgtgcttcgt gctcagtggc agcggtaacg gtgaatacat cgatgttgct gtggtggcac
    40381 tgcataaagc gccagacaaa gtaagggcgt gcggatacag ccatgtgaaa ggctcctatg
    40441 tttgaggtaa ggagtctcgc tactacgctg ctaaacgggg tggcgagacg taacagggtt
    40501 agcagactgg caacatagaa accagcgagc gcaaaggctc ccccgttacg ccccgccata
    40561 atgcgggtat gggtaggctt acggacgcaa aaaaaccgca tatcggaatg taagcggctg
    40621 tccgctatgt tattcaggct gctaaacccg gtcgccatgt ggacgactcc ggcatcatac
    40681 agcccgaaag aagaggcgat caacatgttt ttaagcatga ctcaccccct gacgaatacg
    40741 ggcggcgaat acagcgacac aaccggacgg gcaacggtta cgcgcttcgc gttccgtcca
    40801 ggcggttacg tggatgattt gacgttctga cgtgccaaag gtgataaagc gccagataaa
    40861 ggcggtttga gaatgtacga gggcaggggt agtagccatg atggcagcct cctttatcgt
    40921 tgggatatac ccaccgctgg aggttccaat ctcgctggcg gtggactgca cggagttgga
    40981 actactggcg ataaaggaaa ccagcctgac cgaagtcagc cccatacagc ccaccattga
    41041 taaggtgtgc gtgtgcgccg atacaaaaaa agacgcgttc ggcgtctgta tcgcctttat
    41101 ctccagcggg gttccaatcc cgacacccgt tttatgaggt gcgcgggaat catagccatt
    41161 tttgatactg gcgatcaact gattttctct ggcgggtatt tggcgcggat gcctgttacc
    41221 tgtggcaata tacacacgac tgtatacaat acacatcggt taactcctta tattccgttg
    41281 atggcccgta gtgttattgc attgcgggta ttttttacct gccgttaatt gaatgcggca
    41341 aggcatcacg acggttaacc cgttttgcat aacgtgaggc gttttatttt ctgcgctggt
    41401 ggcttttata tttcacatat gggaatttat tattcctgct gataacgctc ctctactttc
    41461 ttctcaagcc atgatttcac ctcatcctca aaccagccaa cgcggcgtaa tccacgctta
    41521 aagccaggaa taaactcacc agccttaacc attgtgtcaa tgcttgttgt actaaggcga
    41581 agaagatttg cgacttctcg ttttgtaagg attttctttt ctgtaatgtt catacttttg
    41641 cgttcctttt gtttcaggcg ctaacttgcc taaacttgtt caaggatatg caaacacggg
    41701 aaaagctggc attgcggaat attacgaaaa aagcccgtaa tattacgggc ggtcatatgt
    41761 tcagaagtct gtcagtcgtc aacgtctgca agccacttac ctatgcatgt tggtgtggca
    41821 ccgatatcaa cccctgcttt ggttagtgcg cggcttaact tgctgtgacc gttttcaagc
    41881 gcccttcgtg ggtttgacgc ctcttcttct ccggctatgg cggtgattag tcttttgata
    41941 actctggcct gaagattggt tgttttcttg ctctgcgaat gcgacaaggc gtgtttatct
    42001 gaaaaatcct ttaccatatc tcctggttgt ctgccgccgt tcattactat ttttatctct
    42061 tcttttgaga tgaaaacgtc actaatattt attgttttgc catataggtt tattgttatc
    42121 tctggttctt ctgctttatc tgctataaca atgcggcgca cagagcaatt tgtaatttca
    42181 tttgataggt tctctatgtc aatgcttgta ttaaatacct gtaaaagccc tgaagccaca
    42241 accctgattt tatgtggtaa ctgcgttctg atttttcttt gtttatattt ctgataattg
    42301 catataaaaa tatccgtttc ggaataacta ctatcgtaat aaaaaaatac cttttccgtt
    42361 agttggtctt cattgtttgt tattttttca ccaaaatatg aagattcaag tagttttaat
    42421 atgtcggttg ctctgatgaa tacgccttca tcattttcat aataataatc tgatgctgat
    42481 acactattaa atgcgttttt ataattatgt aatgtcaaag cctcgtctat gtgatgacgg
    42541 taccaaaaac acatcttccc tgtagcgatg aagtgaatca agtcatcaac gtcacaccca
    42601 agccttctgg ctgccttgtc taacgggtaa taatccctct caggaaggtt tatttttttg
    42661 tttgccatgc ccttaaccgt taaccatttt taacccacga tagccacccg tggcgcttac
    42721 gctcccgctg gcagcagcct ctacaaaatt cccccaccac gccattaacg ggcgtcgctg
    42781 ctcgagatag gttgagcggt tgtacgcgcg gcgaacctcg tttttgtcta tgtgtgacaa
    42841 tgccgcctcg attacgtcag agtcaaaacc ttgctcgtta agtgcggtac ttgctattga
    42901 gcgcaagcca tgagcaacca gccgccgacc aaaacccatg cgttttattg ctgcgtttgc
    42961 tgtttgtgtg cacatagggc gctgcgggtc gctgttgtgt ggaaatataa actcacgatg
    43021 cccgcttatt ggcttcatga catccagaat ggctaacgcc tgcttcgaga gtggtattac
    43081 gtgttccctg cgttgtttaa gtctgtgctc tggtatgcgc cattccttcg cctcaaaatc
    43141 aatctcacac cagcgcgcgc cagctgcttc acctggtcgg caaattgtca gtaactgcca
    43201 ctcaataacc agtcgcgttt gtcgttccac tcgtgccgtt gacagcttct gcataaattc
    43261 ggggagttct tcagggcgta ttgctggcat gttcttaacg cttggcgcgt taaatgcctt
    43321 gcttatctct gatgcctgat tcgcggcaat cattccggta ttaacggcgt aatacatgac
    43381 ctcattgatt cgctgtgctg tccttctgac ggtatctaat ttcccctggg cggctaatgg
    43441 ttttaatgct tcgatgaaat tgcttgctat caggtctgct attggcctgt taccgactga
    43501 cgggaataca ttacgctcca gcgaacgcca tatatcgcgt agtgtatctt ctgcgagtcc
    43561 ttgccctttt ttaagttcaa accagagcgt agctacatgc ctgaaggtgt tctctctctt
    43621 catcacttca ctaaggcgct gtttctctct gtgctctttt gggtcaatgc caccagaaag
    43681 aagcgctctg tattcgtctt ttatctttcg cgcgcccgca atgctgattt ctggatatgc
    43741 gccaatgctt aacatggccc gtttttttgt gtgtggcttg tagtagcggt aacgccacaa
    43801 gcaagaggcg cttttgtgct taatcaccag ttccagccct tcgccgtcag tgatgaaaag
    43861 gtcctttttt tcgttgctgg cttttcgctg gaggcttttt atcgttgcgg gtgataaaga
    43921 cttaactttt ttggtcaccg gttcaactcc cataagctgt taaaggcata tgcaaatctg
    43981 tgttttttcc ggcgtatgcc tttttgtatg cctttttaat cttgcttata cgctatcaaa
    44041 ctggcccaaa ctgaacaata aaaaacccgc gttccttgtc tggcgcgggt ttgctttgtt
    44101 taacttgccg taacttgtct ttacttggtg tcgatttggt ggcccctgct ggacttgaac
    44161 cagcgaccaa gcgattatga gtcgcctgct ctaaccactg agctaagggg ccatggtagc
    44221 ggattataaa gtaactccgt gtcgcaatcc agccattacc gtgcgcctgc tgtttttata
    44281 aacaatgtat tttcaatccg ttatactttt cctggtgatg ttagaaagga gtaaatatgg
    44341 ttgaggatat tttggctcca gggttacggg tcgtgttttg cggtatcaac cctgggcttt
    44401 catccgccgg gactggtttt ccctttgctc atccagcaaa tcgcttctgg aaggtgatat
    44461 atcaggccgg gtttaccgac cgtcagttga agccgcagga ggcacagcat ctgctggatt
    44521 atcgttgtgg cgtcaccaaa ctggtagacc gtccaacggt gcaagccaat gaagtttcaa
    44581 agcaggagct gcacgcaggt gggcgtaagc tgattgaaaa aattgaagat tatcagccgc
    44641 aggcgttggc gattctgggc aaacaagcat atgaacaggg attcagccag cgcggtgcac
    44701 agtgggggaa acaaacgctc accattggtt cgacgcagat ttgggtgctg ccaaatccca
    44761 gcggtttaag tcgcgtttca ctagagaaac tggttgaagc atatcgcgag ctggaccagg
    44821 cgctggtagt gcgtgggcga taaaaacgcc accgaaaagg tggcgtttgt gcagagggac
    44881 tagtgccggg tgcggcgtaa cgcctgatcc ggcctaccga ttaatcgtcc aggaagctac
    44941 gcaacacttc agaacggctc gggtgacgca gtttgcgcag cgccttcgct tcgatctgac
    45001 ggatacgttc gcgggtaacg tcgaactgtt tacccacttc ttccagcgtg tggtcggtgt
    45061 tcatatcgat accgaaacgc atacgcagaa ctttcgcttc acgcgcggtc aggccagcca
    45121 gcacgtcgtg cgttgccgca cgcaggcttt cggtggtcgc agaatccagc ggcagctcga
    45181 gggtggtatc ctcgatgaaa tcccccagat gcgaatcttc atcatcaccg atcggcgttt
    45241 ccatggagat tggctctttg gcgatcttca gcactttgcg gatcttgtct tccggcatca
    45301 gcatacgttc agccagttct tccggcgtcg gttcacggcc catctcttgc agcatctggc
    45361 gagaaatacg gttgagtttg ttgatagtct caatcatatg caccggaata cggatggtgc
    45421 gcgcctgatc cgcgatagaa cgggtgatcg cctgacggat ccaccaggtt gcgtaggtgg
    45481 agaacttgta accacggcgg tattcgaatt tatcaaccgc tttcatcaga ccgatgttgc
    45541 cttcctgaat caggtcaagg aactgcaagc cacggttggt gtatttcttg gcgatagaaa
    45601 taaccagacg taagttcgct tcaaccatct ctttcttcgc acggcgggct ttcgcttcac
    45661 cgatggacat acgacggttg atatctttaa cctgctcgat ggtcaggccg gtttcttctt
    45721 caatctgctg cagtttctgc agggcgcgat gcacttcttc agagacatcg tgcagttttt
    45781 ccgaccacgg cttgttcatc gcaattgccg cgttgaacca ggtatcgctg gtttcattgc
    45841 cggtaaacag ggtaatgaag tttttcttcg gcattttgca ctgctcaacg cagagcttca
    45901 tgatcagacg ttcttgcgta cgaacgcggt ccatcatgac gcgcatgctg ttgaccaggt
    45961 agtcaaactg cttcggcacc aggcggaact gtttgaatac ttcagacagt ttcaggatct
    46021 cttcctgagc ggcagcgtta ctgcgacctt tcgctttgat ggtgtcacgc gttacaacgt
    46081 actgagcgcg tagttccgca aatttttcgc gagccagttc cgggtcgatg ctgttgtcat
    46141 catcggcgct gtcgtcatcg ccatcttctt cgtcttcatc ttcgtcatcg tccagatctt
    46201 cctgggaaag ctcagaaccg acgtgagtgg cggtaggtgc cagatcttct tctgcgttcg
    46261 ggtcaacaaa gccggtgatc agatcggaca gacgcgcttc ttctgcttca acacgatcgt
    46321 actgttccag cagataggtg atcgcttccg gatattcagc aacggagcat tgaacttggt
    46381 tgatcccgtc ttcaatacgc ttagcgatgt caatttcgcc ttcgcgggtc aacagttcaa
    46441 cggtgcccat ttcacgcatg tacatgcgta ccgggtcagt cgtgcgcccg atttcagatt
    46501 ccacgctgga aagcacctgc gcggcggctt cggcagcatc ttcgtccgcg gtgttttcag
    46561 ccagcatcag atcatcggca tccggtgctt cttccatcac ctgaatgccc atgtcgttga
    46621 tcatttggat gatgtcttcg atctgatctg aatcgacgat atcttccggc agatggtcat
    46681 tgacctcggc ataggtcaga tagccttgct ccttaccacg ggtgacaaga agtttcagct
    46741 gtgactgcgg gttttgctcc ataagacggt atccacactt atttcatgag gttggtgttg
    46801 ggcgattgac ccgatgtcct tcagccgtta acgctgaaat ccaacggttg tttaccgcca
    46861 acgataatta cgagggcgta cttatatatt tgccgctgcc tctcagtgcg gctgtcgggg
    46921 gcttcccgat cgctcttcgg cacttaagcc gttaaatcac tttttcgcca gctcctggtt
    46981 taatgtccag agctccaggc gttcttcgtt gcttaaacca tgcgtgcgct cacgagcgat
    47041 taactcttcc tggcgcagtt caagcagcga atcaaacata tggttgagtg agtcggtgaa
    47101 ggtttgctca gcaatattct tatctgctat atcgtcccac atcgacagtt tttcaagggt
    47161 ggcagcatta tttgtaccac gatagtgctc taaaagttgc ccggtggtca gacctggctg
    47221 ggagagacaa gtgttgacca gttctctgaa taagccaagt ccagggagct tattttcatc
    47281 cagattctca agcggcggga ccaacgtcgc taattctggt ttttgcacca gcaaccctat
    47341 aagtatacgc atggtcgtgc gttttagctg cggaacaggg cgagaaacgc cgctctctgc
    47401 cgcttttggc attaatcgtt caagctggct gtcatcaagt atgcctaatt tgttgcctaa
    47461 ttcctgacga agatatattc gcagcgtttc gcccggcact tgcgatatca atggcagtgc
    47521 cagcgtactc aaacgtgcgc gcccgtcagg ggtactcaga tcaacttgcg gcatcaggct
    47581 gttaaacaga aatgcggaaa gcggcatcgc ctgctccatc cgcgcttcaa acgcttcttt
    47641 accttctttt cgtaccagcg tgtcagggtc ttcgccatcc ggcaaaaaca taaagcgtag
    47701 ctgacggccg tctgtcatgt aaggcagcgc cgtttccagc gctcgccatg cggcatcgcg
    47761 gcctgcacgg tcgccgtcat aacagcaaat gacattgttg gtcgcgcgga acaacagttg
    47821 tatgtgatcg gcggtggttg acgtacctaa cgacgcaacg gcgtaattaa tgccgtattg
    47881 cgccagcgcc accacgtcca tatagccttc gaccacaagc agacgattgg gttcagcgtt
    47941 atcctgctgc gcttcataaa gaccgtaaag ctggcggcct ttatggaaaa tgtctgtttc
    48001 cggcgagttc aggtatttgg gggtatcgtt gcccagcacg cgcccgccaa aaccaatcac
    48061 ccgaccgcgt ttatcgcgaa tggggaacat cacccgctcg cggaaacgat cgtaactgcg
    48121 tccctgatcg ttagtgacca acatccccgc atcaatcaat gactggcgat tttctggatt
    48181 gccgccaaac cgcttcagga cgttgtccca gccggggggc gcaaaaccaa tcgcaaagcg
    48241 agcgataacc tcgtggctta atccgcgttt ttccagatac tggcgcgcag acgtggcaac
    48301 aggttgttgt aaagattgtt ggtaaaacgt attcagaccg tccatcaact gataaagcgt
    48361 ttgcctctga tggcgctcga tctggctggg gccgctgcct gcttcaaatg gcacttcaag
    48421 attgtgcatt gctgccagct cttcgaccgt ttcaacgaac tcgagcttgt cgtagttcat
    48481 caggaagtcg atcgcgttgc cgtgcgcgcc acatccaaag cagtggtaaa actgtttctc
    48541 accgttaacg gtgaatgacg gggttttctc gttgtggaat ggacaacacg cgtggaaatt
    48601 cttgccctgc tttttcagct tcacacgggc atcgatcaga tcgacgatgt cagtgcgtgc
    48661 cagcagatca ttaatgaata cgcgtgggat tcgtccagcc ataggccccg atttttagca
    48721 attcataaac gaaaataagc cgcgcattcc tttcggaagc acggccttac aactacaact
    48781 cggtctgatc ggagagcaac gctctcgggg aattagtaca gacgagtgcg gcgtgcgttt
    48841 tcgcgagcca gtttcttcgc gtgacgtttc actgcagaag ctttagcgcg cttacgttcg
    48901 gtagtcggtt tttcatagaa ctcacgacga cgaacttccg ccagaacacc tgctttttcg
    48961 caggaacgct tgaagcgacg cagagctacg tcgaacggct cgttttcacg tactttaatt
    49021 accggcatgt gcctctcacc tttgattaat tcggtttgcc gctggcatca acgccagctt
    49081 atttcaaaat ggtgcggaat tttactgcaa ttgctgctgc tttgtaaagc accgcggcct
    49141 tttttgaaag ggacttttat aagggtgagg agtatacacg aggctttctc caggggcgaa
    49201 caaagtttta catcaacccg cattggtcct acactgcgcg gtaataaagc gaggtaaaac
    49261 aagtcatgcg tgtactgggt attgaaactt cctgcgatga aaccggcatc gccatttacg
    49321 acgatgaaaa aggtttgtta gccaaccaat tgtatagtca ggtgaaattg cacgctgact
    49381 acggcggcgt cgtgcctgaa ctggcctccc gcgatcacgt gcgcaaaacc gtaccgttga
    49441 tccaggcggc gctaaaggag tctggtttaa cggcaaaaga cattgatgct gtggcctata
    49501 ccgcaggccc tggattagtc ggcgcactgc tggttggcgc aaccgtgggg cgttctctgg
    49561 cgtttgcctg gaacgttccg gcaatcccgg tacaccatat ggaagggcat ctgttagcgc
    49621 cgatgctgga agataacccg ccggaatttc cgtttgtcgc gctgctggtg tccggcggtc
    49681 atacgcagtt aatcagcgtg actggcattg gtcagtacga gctgctcggc gagtctatcg
    49741 atgatgccgc cggtgaagcg tttgataaaa ccgcgaagct gctggggctg gattatcctg
    49801 gcggaccgtt actgtcgaaa atggcggctc agggtactgc cgggcgcttt gttttcccgc
    49861 gtccgatgac cgaccgtccg gggctggatt tcagcttttc tggtctgaaa acctttgcgg
    49921 cgaacacgat tcgtgacaac ggcaccgacg accagacgcg tgctgatatc gcccgcgcct
    49981 ttgaagatgc ggtggtcgat acgttgatga ttaagtgtaa gcgagcgttg gatcagacgg
    50041 gctttaagcg actggtcatg gcgggcggcg tgagtgctaa ccgcacgtta cgggcgaagc
    50101 tggcggaaat gatgaaaaaa cgccgcggcg aagtgttcta cgcgcgtccg gaattttgta
    50161 ctgataacgg cgcgatgatc gcctatgccg gaatggtgcg gtttaaagca ggcgcgacgg
    50221 cggatctcgg cgttagcgtg cgtccgcgct ggccactggc agagttaccg gctgcgtaaa
    50281 actatccggt gccggagagc aatttccggc accgtcctca cttaaagcaa caccacgggc
    50341 atccacagca agccggtaat caccagcaac acgaggaata tcagtccaaa aatcgccccc
    50401 agtcgccagt aatccaccgt tggcagataa ccactaccgt agtaaatcgg actgggtccg
    50461 gtggcgtata gtgtaagaat gctccccaga ccaattgccg cgccaaccat caggcagaac
    50521 accggcagcg ggatttccgg catcgccagc gcagcggcaa tcatcatcgg tgcgagggcg
    50581 gaggtatacg ccgtggcgct ggcgaaaaag tagcgcagca gataaaacac cacaatcaac
    50641 gctaccatca ccatcgttgg cgaataaccg cttaagctgc ctgctaacag tttgccaaac
    50701 cagctaataa aaccagtgtt gttgagtccg gtagcgaggg tgataagcga ggccagccag
    50761 aagaaaacgt tccacgccgc tttattactg acaatgtcgt cccaactgat aatgcgcaga
    50821 agcagcatca gtgccaccac gctgtaaccg accatcgcgg catcgatata atcaccgccg
    50881 aaaatccaca gcaccagcgc gcctaccatc agccccagca tccgtttttc acgcgaacag
    50941 agcgggccca ttgcctgcag ttccgtctct gcccagcgcg gcacctgatc gccagacttc
    51001 agtaccggcg ggtacagcac ataagccagc cagggaacca gcagcaccag caaaatactg
    51061 agcggcaaca tcccgaggaa ccagtcgccc caactcagcg tggcgtgaga tgcgcttttc
    51121 atcagtccaa ttaacagcaa gtttggtgcc atcgccgtca agaaaatggc gctggtcacg
    51181 cagtcggcaa caatccccat ccacatgatg tacgagccaa tgctgcgcga actgctgtcg
    51241 tttggttgtg attgatagag cggtggcagg ttacggatga tgggatagat aatccccgca
    51301 ccacgcgccg agttggacgg tgttacaggt gcgaggatca gctcggagaa catcaccgca
    51361 tagccgagaa acagcgtgcg atgtcccatc tttttcacca gaaacagcgc aatgcggcgt
    51421 ccaagcccgg ttttttcata gcctgtgcca aacataaagg cggcgaaaat cagccagata
    51481 accgaattag aaaaaccgga aaccgcccac gagagggatt ttgcagtgaa tttaaagcct
    51541 ggctgggcga gctgttcggg gctgaacagc agccagggag agagtatggc gatgatggag
    51601 atacccacca tcgccaccac ggcacccggc acgggttcga ggatcagtcc aacgatcacg
    51661 ccagtaaaaa cggcaaagta gagccaggta tggttctcca gccctgcggg aaccggaagt
    51721 agagcaataa tggcgatgac cgccagcggc gcaagatatc gccaccattc agtggaaggt
    51781 ttcatcgtgt tatgcctgcg aaaaaggaga ggggtgagcc tctccgggag ggttatttga
    51841 tgtaatggac gtgctcgcag atctcttcca cgatgggatc gcggcgttcg gcgaacagct
    51901 ttttgttttc ggctatcagg ttgttgccgt gggtatcaat agagacaatc agcgggccga
    51961 actctttgac ccggcagacc cacagtgact ccggcatccc gagttctgtc cagtgcactt
    52021 cttcaatctc ttctacctgg gttgccgcca gcaccgcgca gcctgccggg aaaatcacat
    52081 gtagcgcctt gaatttctgg cagccttctt cggtcagcgg ccccatacca cctttgccaa
    52141 ccaccagttt cacgccggtc tgctcgataa attcacgttc aaaactttcc atacgcatac
    52201 tggttgtcgg gccaacggaa accatctccc atttatcgcc gtttttgcgc acgatggggc
    52261 cagcgtggaa aatcgctttg ccgttgagat cgtaagggat cggacgtttc agttcgatca
    52321 agcggcggtg acaaacgtca cggcaggtca ccagcgtacc ggtcaggtag atcacatcgc
    52381 cgacgcgaat atcttgcaga tcttcagctt tgatcggggt tgtcaggatc tttttcataa
    52441 cgcgctccgg gtgtgagaca gattttcaaa ggtgagatcg gcatgaacca gcagtgtgcc
    52501 gcgacgatgc gcccagcagc ctgtagaaac agcaacgccg atggttgacg gatggcgggc
    52561 ggcagattcg atatgcacgc ccatcactga actgttgcca gtcagccctt gtgggccaat
    52621 tcccagacgg ttgagtcctt cttccaggcg tagctccagc tctgccgctt ttggattggg
    52681 atggcggctg ccaatcgggc gcaaaatggc ttttcgcgag agcacggcgg cggtttccac
    52741 cgaggtggcg atgcccacgc ccaccagtac cggtggacag gcgtttacgg cgagggtgga
    52801 gatattttcg aagacgaatt tcactacgcc ttcgtagcct tctgacggca ttaacacttt
    52861 cgagcggcca ggtagcgtgc agccgccgcc tgccatgtaa acttcgattt ccgcatcgtc
    52921 attgtcgggg atgatgtccc aggtgaccca cggtacgccg ctaccggtat ttttgccggt
    52981 gtttacttcg tcaaaaattt ctaccgcatt gtgacgtagt ggcgctttga cggttgcctc
    53041 ttccacggct tgtttgagta tgctttgcag ctcgccaagc agtgggaagc gggaaccgac
    53101 tttaacgaag aacataatct ccccggtgtc ctgacaggca ggacgattca ggtcaatcgc
    53161 tttttgcatg ttgtcgaaca tcgtatggta gataattttc cccatcgacg acgtttcggc
    53221 atcctttagc tgttttagtt tatccaccac gtcatcaggc attcgggtag aaatcatggc
    53281 ggtaaagtta gcgacaatct ctgtcaactt attcactgcc tgttgcttat tactttcgct
    53341 catcatggca actccagttt ttgtatttgt ctggaaaaat tcaccgccac aaactaccac
    53401 taaaaatgtg gttttatatt ttttgccacc gtgaatatgt aattaacagt tcgtgaatca
    53461 acgcgaaaat aagaatgaat attgcgccag agcagggaat ggcaatgttt tgcgaaatac
    53521 actgcgctta tcacaactgc ggataattaa cgatgctgaa tagctggcct ttagccaaag
    53581 acttgcaggt actggtagaa atcgtccaca gcgggagttt tagcgccgca gcggcgacgc
    53641 ttgggcagac gcctgcattt gtcaccaagc ggatccaaat ccttgaaaat acgctggcaa
    53701 caacgctgct gaaccgctcc gctcgcggcg tggcgctgac tgaaagtggc caacgttgtt
    53761 acgaacatgc gctggaaatc ctcacccaat atcagcgact ggtcgatgac gtcacgcaaa
    53821 tcaaaacgcg cccggagggg atgattcgta ttggctgtag ttttggtttt gggcgcagcc
    53881 atattgcgcc agccattacc gaacttatgc gcaattatcc tgagttacag gtgcattttg
    53941 aattgttcga tcggcaaatt gatttagtgc aggataatat tgatctggat attcgtatta
    54001 atgacgaaat tcctgattat tatattgcgc atctgttaac gaaaaataaa agaatattat
    54061 gcgcagcacc tgagtatctg caaaaatatc cgcagccaca atcattacag gaattaagtc
    54121 gtcatgactg tctggtgacc aaagagcgcg atatgaccca cggaatatgg gagttgggga
    54181 acggtcagga gaaaaaatcg gtgaaggtta gcgggcatct ttcctccaat agcggcgaga
    54241 tagtgttgca atgggcgctg gagggcaaag ggattatgct gcgttccgag tgggatgtgt
    54301 tgccgtttct ggagagtggc aaactggtgc aagtattgcc ggagtatgca caaagcgcca
    54361 atatctgggc tgtttaccgg gagccgctct atcgcagcat gaaattacgt gtctgcgtgg
    54421 aatttctggc ggcatggtgc cagcaacggc tgggcaagcc cgatgaaggc tatcaggtca
    54481 tgtagatcca ccagaaatca ctcgggatcc ttttcgcgct ttcttttgaa tttcgtccag
    54541 atttttgtct cctgacgacg ccacagacgc tggatgttgt catgatgacg cagcaggatc
    54601 aggcaagaga gcatcgaaac cgggaaggtg aattgtggct taaaccacca tacataaaac
    54661 ggagcaatca gtgcactgac aatcgctccc agcgacgagt atccgctcaa tagcacggtc
    54721 agtaaccagg ttcccgccat tactccggtg agatcccagc caatgggtgc gatggcacca
    54781 aaagcggtag caacgccttt tcctccttta aatccgaaga aaacgggcca gatgtgtcca
    54841 agacaggcgg cgatggcaat taagcctagc cagaaggggc tgacacctaa ttcatacgcg
    54901 ccccagacgg gcaacattcc tttcagaacg tcgaaaatca gtactgctac ggctgctccc
    54961 ttgccaccga tacgtaacac attggttgcg cctggattgc cggagccgct ggttcgcgga
    55021 tcgggcagcc cacacaagcg gcaaaccaga atggcactgg aaatggagcc gcagaggtac
    55081 gcgatgagga tcattccagg cgcgattgca ctcataagct gttccgtttt gaaaattcgt
    55141 gttcaacgat gaatctgtgg ataatacgca catttcgccg gaagtggtat ccggttagcc
    55201 aaaaagcagg caggacgtga tggatattgt atttatagag caactttcgg taatcaccac
    55261 tattggtgtt tacgactggg aacagaccat cgaacagaag ttagtgttcg atatcgaaat
    55321 ggcatgggat aaccgtaaag cggcgaaaag tgatgatgtg gcggattgcc tcagttacgc
    55381 tgacattgca gaaacggtgg tcagccacgt cgagggggcg cgttttgcgc tggtggaacg
    55441 cgtggctgaa gaggtggcgg agctgctgtt agcacgcttc aactcgccgt gggtgcgtat
    55501 caaactcagc aagccaggcg cagtggcgcg ggcggcgaat gttggcgtaa tcattgagcg
    55561 tggcaataat ctgaaagaaa ataattaatt ttacagctgt taaaccaaac ggttataacc
    55621 tggtcatacg cagtagttcg gacaagcggt acattttaat aatttagggg tttattgatg
    55681 agcgatatgc actcgctgct gatagcggca atattgggtg tggtcgaagg attgacagaa
    55741 tttctgccgg tatccagcac gggccatatg attattgtcg gtcacttgtt ggggttcgag
    55801 ggcgacacgg cgaaaacctt tgaagttgtg atccagttag gatcaattct ggcggtagta
    55861 gtgatgttat ggcggcgtct gtttggcctg attggcatcc actttggccg cccgttgcag
    55921 cacgaaggag aaagcaaagg tcgtttaacg ctgatccaca ttttgctggg gatgattccg
    55981 gcggtggtac tggggctgtt gttccacgac acgattaagt cattgtttaa cccgataaat
    56041 gtgatgtatg cactggtcgt tggcggtttg ttgctgattg ccgccgaatg cctgaagccg
    56101 aaagagccgc gtgcgccggg tcttgatgat atgacctatc gtcaggcgtt tatgattggc
    56161 tgtttccagt gtctggcgct gtggccgggt ttctcccgtt ccggggccac catttcaggt
    56221 gggatgctga tgggcgtgag ccgttacgct gcttccgagt tctcgttcct gctggcggta
    56281 ccgatgatga tgggcgcaac ggcgctcgat ctctacaaaa gctggggctt cctgacaacc
    56341 ggcgatatcc cgatgtttgc cgttgggttt atcaccgctt ttgtggtggc gctgatagcg
    56401 attaaaacct tcctgcaatt gattaagcgc atttcgttta tcccgttcgc catttatcgc
    56461 tttattgtgg cggctgcggt gtacgtcgtg ttcttttgat tgagaaaagg ttcgggagca
    56521 ggcctggttt tctccctcgc tctgtaggga gagggccggg gtgggggtga ttgttcgcac
    56581 cggtgctggt ctgttcccct caccctaacc ctctccccga aggggcgagg ggatggttcg
    56641 tgttaacgtt tagttttata ctcagtcagg ctttgggcaa cgttgttcct tccaactggc
    56701 taccgccgca atccgccgtc gggtcagctc ctcgcgaatc tccacacctt taaatcccgc
    56761 ttcaacgacc gcttttgtcg gcactgactg cgccacttcc caggcttcgc gcaaccaacg
    56821 gccttgcggg tagtccgctg attcaaaacc ggttctgccg cgcacgtcag cctcgctggt
    56881 cagcgccagt tgctcgacac gctgcggttt acgccaggca tcgatggaat caaacaattt
    56941 gacgatagtt tttgggttca gcattgggaa ggtgtggatg agatcgtgaa actcagccac
    57001 cagtctggct aaatcgcgaa tttcatttgg cacgcgcaga cgctggcaga gttgttccac
    57061 taatttcaca cccgccggac cgtgaccatg atgacgcggc caaagttctg gcggcgtcag
    57121 tcctttaccg agatcgtggc ataaagttgc gaaacggaca tcgacctgcg gactcagcat
    57181 cgccgccatt gagagcgtca ttaaggtatg aatacccgtg tcgatttccg gatgccacct
    57241 ggcaggggcc ggaacgccaa acagtgcgtc aatttccggg aataaaacgc gcagtgcgcc
    57301 acaatcgcgc agtacctgga agaacacctg tggattgcgg gtggtgaggg cgctttccgt
    57361 ctctttccat acccgttcag gcgtcaggtg ttccagttca cccgcatggg tcatctcgcg
    57421 catcagcgtc agagtttcat cggcaatacg aaaaccgagg tgggcataac gcgcagcaaa
    57481 acgcgccacg cgcagaacgc gtaacggatc ttcgccaaaa gcgggggaaa catggcgcaa
    57541 cagacgattt tgcagatcgc ccagaccgtt gtacgggtcg ataatctcgc cgttatcgtc
    57601 ctgggccagc gcattaatgg tcagatcgcg acgcttaaga tcatcttcca gcgtgacatc
    57661 cggtgcggca tagcaagtaa aaccggtgta accggaaccg gatttccgtt cggtacgtgc
    57721 cagcgcatac tcttcatgcg tttgcggatg cagaaacaca ggaaaatcgc ggcctacctg
    57781 ctggtagccc gcgtcgagca tctcctgtgg cgtactgccg accaccaccc aatctctgtc
    57841 tttgaccggt agccctaaca atgcatcccg aacagcacca ccgaccagat aaatcttcac
    57901 gccatcatcc cttcgtcaaa tacacaattc ctaataataa gacagtgcaa cgaagaaggc
    57961 gatttagttc atccagcgat ctttgcgttt gcgcgttggg atcaggtgtg gcagtaccag
    58021 accgagcagc aagccaagcc ccagcacgcc accgccatac ataaaccatt gcatgatgat
    58081 ggtgcgctgt ttgtcatcca actgtacgct ggcggcatcg acctttttct gcgcgacaat
    58141 cagctcgttt ttcaatttct gattttcttc ttttaatccg ttgatcacgc tgtcgctctg
    58201 cgccactttt tgctgcattt ctgcggtgcg ctgattccag gtgttatcga tattggtgag
    58261 tttatcggtc agggttttga cctgattttc cagatctggc acacgggagc gcaggcttgg
    58321 ctcagtgcta agttgtttca acgggatcca ggcggtacgg ccagagctgt ctttcacctg
    58381 ggcataattg gtgttggcgt cagtttgtaa taaggtcact tcctcgccgg cgttaaccgt
    58441 gcccacgagg cgataatgat ctcccggacc gctacggacc caggtattca gttcatcaga
    58501 aacatagcgc gtttcttcag cgtgtgagac ggcagtggcg ctaagtgcga gtaaagttaa
    58561 tccgatcagg cgtaattttg gcatcaggct gtcgttattg tcatggaaag tggaacgata
    58621 gtagtggcat cagtgtcgct acgcaaagca ttcgacatca atcggaatcc tctgtgtcag
    58681 gtcttgccac tgcttcaaac ttttacgccc gtcaaattgc ccgttgcatg gcaatttggc
    58741 gcaaaatact atctactgac aaaaaagatc gtattaagtt cgccgttcat cgtcacttat
    58801 gtgacataac cgataagtaa aggccatggc tcaggaaatc gaattaaagt ttattgttaa
    58861 tcacagtgcc gttgaggcgt tgcgtgacca tctcaatacg ctgggcggcg agcaccatga
    58921 ccccgtgcag ttactgaata tttactacga aacgccggat aactggctgc gtgggcacga
    58981 tatgggctta cgtattcgtg gcgaaaacgg tcgctatgag atgaccatga aagttgcagg
    59041 aagagtgaca ggcggcttac accagcgccc ggaatataac gtggcgttga gcgaaccgac
    59101 gctcgacctg gcgcagttac cgacggaagt ctggccgaac ggcgaattgc ccgccgatct
    59161 cgcttcccgc gtacaaccgc tgttcagcac cgatttttat cgcgaaaaat ggctggtggc
    59221 ggtcgatgac agccgaattg aaatcgctct cgatcagggg gaggtgaaag cgggggaatt
    59281 tgctgaacct atctgtgagc tggaactgga actgcttagc ggcgacacgc gcgcggtgct
    59341 gaaactggcg aaccaactgg tatcgcaaac cggattacgc cagggcagcc tgagcaaagc
    59401 ggcgcgtggc tatcatctgg cgcagggcaa tccggcgcgt gaaatcaaac cgaccaccat
    59461 tttgcatgtt gcggcaaaag ccgatgtgga acaggggctg gaagcggcgc tcgagctggc
    59521 gttagcgcaa tggcagtatc atgaagaact gtgggtacgc ggcaacgatg cggcgaaaga
    59581 gcaggtgctg gcagccattg gccttgttcg ccatgcactg atgctgttcg gtggcattgt
    59641 gccgcgtaaa gcgagcactc acttacgtga tttgctgact caatgcgaag cgaccatcgc
    59701 ttctgcggtt tctgccgtga cggcggtcta ctctaccgaa acggcaatgg caaagctggc
    59761 gttgaccgaa tggttggtaa gcaaagcctg gcagccgttt ttagatgcca aagcgcagag
    59821 taaaatgagc gattccttca aacgctttgc cgatatccat ctttcccgcc atgccgctga
    59881 actgaaaagc gttttctgcc agccgttggg cgatcgctac cgtgaccagt tgccacgtct
    59941 gatgcgtgat attgactcaa tactgttgct ggcgggttac tatgatcctg tcgtcgcgca
    60001 agcctggctg gagaactggc aggggctgcg tcacgctatt gcgaccgggc aacgcattga
    60061 aattgaacat ttccgtaatg aggcaaacaa tcaggaaccg ttctggttgc acagcggaaa
    60121 acgttaatca ggcaaggata aaatcgctta tgaagccgct ctcttcaccg ttacagcagt
    60181 actggcagac cgttgttgag cggctgccag agcctttagc cgagaaatca cttagcgcac
    60241 aggcgaagtc agtacttact ttcagtgatt ttgtgcagga cagcgtgatt gcgcatccag
    60301 agtggctgac ggaactggaa agccaatcgc cgcaggctga tgaatggcag cattacgcgg
    60361 catggttgca ggaggcgctc agcaatgtga gtgacgaagc cgggttaatg cgcgagctgc
    60421 ggctattccg gcggcgcatt atggtgcgca tcgcttgggc gcaaacgctg gctttggtta
    60481 ctgaagagag catattgcag cagctcagcc atctggcgga gacgctgatt gtcgccgcgc
    60541 gtgactggct gtatgacgct tgctgccgcg aatggggaac gccgtgcaat gcgcagggcg
    60601 aagcgcaacc gctgctgatt ttaggcatgg gtaagctggg cggtggggaa ctgaatttct
    60661 cctcagatat cgatctgatt tttgcctggc cggaacatgg ttgtacgcag ggcggacgcc
    60721 gggaactgga taacgcgcag ttttttaccc gcatggggca gcggctgatt aaagtgttgg
    60781 atcaaccaac gcaggatggc ttcgtctatc gcgtggatat gcggctgcgt ccgtttggcg
    60841 aaagtggccc gctggtgctg agctttgccg cgttggagga ttattaccag gagcaggggc
    60901 gcgactggga gcgttacgcg atggtcaagg cgcggattat gggcgatagc gaaggcgtct
    60961 atgctaacga gttgcgtgcg atgctgcgcc cgtttgtttt ccgtcgttac atcgatttca
    61021 gcgtgattca gtcgctgcgc aacatgaaag ggatgattgc ccgtgaagtg cgtcgacgtg
    61081 gtttgaccga caatatcaaa ctcggcgcag gcggcattcg cgaaattgaa tttatcgttc
    61141 aggtgttcca gctcattcgc ggtgggcgcg aaccgtcgct gcagtcgcgc tctttactgc
    61201 caacgctcag cgccattgcc gcgctgcatc tgctttctga aaacgatgct gaacaattgc
    61261 gagtggcata tctgttcctg cggcgtctgg aaaacctgct gcaaagcatt aacgacgaac
    61321 aaacccaaac gcttccttct gatgagctta accgtgcgcg gctggcgtgg gcgatggact
    61381 ttgctgactg gccgcaactg accggggcgc tgaccgcaca tatgaccaat gtgcgccggg
    61441 tgtttaatga attgattggc gacgacgaaa gtgaaactca ggaagagtcg ctgtcggaac
    61501 agtggcgtga gctgtggcag gatgcgttgc aggaagatga cactacgcca gtgctggcgc
    61561 atcttagcga ggatgatcgc aaacaggtcc taacgctgat tgctgatttc cgcaaagagc
    61621 tggataagcg tactatcggg ccgcgaggac gtcaggtgct cgaccatctg atgccacatc
    61681 tgctaagtga tgtctgtgcg cgtgaagacg ctgccgttac gctgtcgcgc attaccgcct
    61741 tgctggtggg gattgttacc cgcaccacct atttagaatt gctcagtgaa ttccgtgcgg
    61801 cgcttaaaca tttgatttct ctgtgtgccg catcgccgat gattgccagc cagctggcgc
    61861 gttatccatt attgctggat gaattgctcg atccaaacac tctttaccag ccgacggcga
    61921 ccgatgccta ccgcgatgag ttgcgccagt atttgctgcg cgtgccggaa gatgacgaag
    61981 agcaacagct tgaggcgctg cgtcagttca aacaggcgca gctgttacgc atcgccgcag
    62041 cggatatcgc tggtacgcta ccggtgatga aagtgagcga tcacttaacc tggctggcag
    62101 aggctatgat agatgccgtc gttcagcagg cgtgggttca aatggtggcc cgctacggta
    62161 agccaaatca cctcaacgat cgcgaaggcc gtggttttgc ggtggtcggc tacggcaagc
    62221 tgggcggctg ggagttaggc tacagttccg atcttgacct gatcttcctc catgattgcc
    62281 caatggatgc gatgactgac ggcgagcggg aaatcgacgg gcgacagttt tatctgcgtc
    62341 tggcgcaacg cattatgcat ctgttcagta cgcgaacctc ttccggcatt ttgtatgaag
    62401 tggatgcacg actgcgtccg tccggggcgg cgggaatgct ggtgacatcc gcagaagcat
    62461 ttgctgatta tcagaaaaac gaggcctgga cgtgggagca tcaggcgctg gtgcgtgcgc
    62521 gtgtagtgta cggcgatccg cagctcaccg cgcactttga cgcagtgcgt cgcgagatta
    62581 tgacgctgcc gcgtgaaggt aaaactctgc aaacggaagt gcgggaaatg cgcgagaaaa
    62641 tgcgcgctca tctcggcaat aaacatcgta atcgctttga tatcaaagcc gatgaaggcg
    62701 gaattaccga tatcgaattt attacccaat atctggtgtt gcgctacgct catgaaaaac
    62761 cgaagttaac gcgctggtca gacaacgtgc gtattctgga gctgctggcg caaaacgaca
    62821 ttatggaaga gcaggaagcg atggcgctga cccgtgctta cacgacgctg cgcgatgaac
    62881 ttcatcatct ggcattacag gaattgccgg gccatgtgtc ggaggattgc ttcaccgcag
    62941 agcgtgatct ggtgcgggca agctggcaga agtggctggt ggaagaatga agtatggtat
    63001 tatcgcgcgc aaattttgaa tctctcagga gacaggaatg aaagtaacgc tgccagagtt
    63061 tgaacgtgca ggagtgatgg tggttggtga tgtgatgctg gatcgttact ggtacggtcc
    63121 caccagccgt atctcgccgg aagcgccggt gcccgtggtt aaagtcaata ccatcgaaga
    63181 acgtccgggc ggcgcggcta acgtggcgat gaatatcgct tctctcggtg ctaatgcacg
    63241 cctggtcggg ttgacgggca ttgacgatgc ggcgcgtgcg ctgagtaaat ctctggccga
    63301 cgtcaacgtc aaatgcgact tcgtttctgt accgacgcat ccgactatca ccaaattacg
    63361 ggtactttcc cgcaaccaac agctgatccg cctggatttt gaagaaggtt tcgaaggtgt
    63421 tgatccgcag ccgctgcacg agcggattaa tcaggcgctg agttcgattg gcgcgctggt
    63481 gctttctgac tacgccaaag gtgcgctggc aagcgtacag cagatgatcc aactggcgcg
    63541 taaagcgggc gttccggtgc tgattgatcc aaaaggtacc gattttgagc gctaccgcgg
    63601 cgctacgctg ttaacgccga atctctcgga atttgaagct gttgtcggta aatgtaagac
    63661 cgaagaagag attgttgagc gcggcatgaa actgattgcc gattacgaac tctcggctct
    63721 gttagtgacc cgttccgaac agggtatgtc gctgctgcaa ccgggtaaag cgccgctgca
    63781 tatgccaacc caggcgcagg aagtgtatga cgttaccggt gcgggcgaca cggtgattgg
    63841 cgtcctggcg gcaacgctgg cagcgggtaa ttcgctggaa gaagcctgct tctttgccaa
    63901 tgcggcggct ggcgtggtgg tcggcaaact gggaacctcc acggtttcgc cgatcgagct
    63961 ggaaaatgca gtgcgtgggc gcgcagatac cggctttggt gtgatgaccg aagaggaact
    64021 gaagcttgct gtagcggcag cgcgtaaacg cggcgaaaag gtggtaatga ccaacggtgt
    64081 ctttgacatc ctgcacgccg ggcacgtctc ctatctggca aatgctcgca aactgggtga
    64141 ccgtctgatt gtcgctgtga acagcgatgc ctccaccaaa cggctgaaag gggattcccg
    64201 ccccgttaac ccgctcgaac agcgtatgat tgtgctgggc gcactggaag cggtcgactg
    64261 ggtggtgtcg tttgaagaag acacgccgca gcgcttgatc gccgggatcc tgccagattt
    64321 actggtgaag ggcggcgact ataaaccaga agaaattgcc gggagtaaag aagtctgggc
    64381 caacggcggc gaagtgctgg tgctcaactt cgaagacggt tgctcgacca ctaacatcat
    64441 caagaagatc caacaggata aaaaaggcta accggaaagc ggttcacaga tctcgcttcg
    64501 cagcaggcag gcctattgac gctgttgttt tttcaaccta aagtaaagga acaagggtaa
    64561 gggaggattt ctcccccctc tgatcagttg ttagtaagtc gggaaactta acagtaacaa
    64621 cacaaccagt atgatgacga acttcatcat aaccctttcc ttctataagg ccccttcctc
    64681 gggaggggct ttcccgtttc agccccttgc tgactccccg attgttgatg agcgccggac
    64741 cccgcgcaac gctttccctg aagcagtttt gccttccccg agcggtcacg caaaacgctg
    64801 caacgtcctg agcatttgca ccttttagcg gaacgcagat ataaaaaatc cccggctttt
    64861 tgggccaggg atatgtgtta atgaaacctt cttgacgctg attttttttc aacctaaagt
    64921 aaaggaacaa gggtgaggga ggatttctcc cccctctgat tggctgttaa taagctgcga
    64981 aacttacgat taacaacaca atcagtatga tgacgagctt catcataacc ctttccttct
    65041 gtaaggcccc ttcctcggga ggggctttcc cgtttcagcc ctttgctgac tccccgattg
    65101 ttgatgagcg ccggacctcg cgcaacgctt tcccagaggc agttttgcct tccccgagcg
    65161 gtcacgcaaa acgttgcaac gacctgatca tttgcagttt ttagcggaac gcagatataa
    65221 aaaatccccg gctttttgtg ccagggacgt tcatagctga aaatttattc tacgttttcg
    65281 gcgacgggag ttgttgaggt taagggtgaa gtcaatgccg ccagtgagcc gcctgaaacg
    65341 ccaatttcat tgagcaagga gtcaatcagc ggtgcctgtg tgcggtaaga gagagcggct
    65401 gataatgctt gttccgccag gtttccgccc cccacattac ccgtgtttgc atcacccgca
    65461 gcgctgccac gattcagacc atcgacctga ataatcttga taccgtcaat agatttcatc
    65521 ggctcaacgg atttttctat taccgcaggt agcgcctgca acaaggccag tttgaattta
    65581 aggctggttt gttcatcaga aagtacgttg atagcatcgt tcagcgcacg ttgtgcttct
    65641 gcttccgcca gacccttttt acgtgtagct tcggctaact caacgatagc cgccgcctgc
    65701 atctctgcgg cttctttttc tgctttcgcc cgcacggtca gttcaaccgc tttggtttct
    65761 gcatcctgcg cggcagcgat tagggcaact tgtttagcac gatcggcttc tgcggtctgg
    65821 cgagtggttt ctacgttttg ttgcgcactt actgcttctg cgagtgcaag gttagcacgc
    65881 gcttctgcct gggactgttg ttccgatttg gcggcaatag cgatcgattt cgtctggttg
    65941 gcgatttcgg tgacctgctg ttgttcgatc tctttaatac gaacttcacg ttcagcctca
    66001 acctttcttg agcggacggc ctgctcgcgc tcgatctccg tttcctgaat ttgtcgttca
    66061 gccagaattc gcgtctgctc tgcttcacga cgacgttcag cttcaaaagc cgcaatacgt
    66121 gcattctgtt cggcagtacg ggttttaacc tgctgctcct gctcaagcgt cataaacgct
    66181 tcttgttgtt caatctccaa cttgcgcgaa agtgcatcac ggtttttctc acgcaccgca
    66241 acttctacat cctgttcaac ttcgttacgt tcgcgacgac ggcgctccgt ctcctgagtc
    66301 agtttggtta aaccttcggc gtcaaaggca ttgttcggat tgaaatgttc tttcgaggtc
    66361 tggttaaagt tggtaagtga aacgctttcc agctcaagac cgtttttcga caggtcttct
    66421 gcgacagtat tttgtactcc ctgcacgaag ttctcgcggg tatcctgtaa ctcatgcatg
    66481 gtcatttgtg cagctgttgc acggagggca tcgacaaatt tatcttcaac caacatacgt
    66541 aagtcttctg gtgatagcgt gcgttgcccc aacgtctgcg ccgcagtggc gatgccttct
    66601 acagacggtt ttacccgtac aaagaaagcg actactacat cgacacgcat acgatctttc
    66661 gtaatcaggc tatcaatggt tgagcggctg acttccagct tcagagtatt catattgatg
    66721 gggattattt catgaaagat cggcatcacg attgcgccac cgctcatcac cactttttgc
    66781 ccacctaaac cagtacgaac aaaagcttgc tctgccgaag cgcgacgata gagcctggcg
    66841 aaaataattc caataataaa cagaatgcat acggcaataa tcgcggtaaa catccaggag
    66901 ggcacagaat taacaatatc atccattatt aaatttcctt ttgattaata aattacaata
    66961 tttgtggcca gggattgttt tccgccaaat atcgcgttgc agaaagtcgg caaataatta
    67021 acactttgtc tcctttagtg aaaaattttc cttcttcagg ctccagtagt aaataatgta
    67081 tttggccaaa ttgatcggtg agttttcctt cgcagggatt gcctgaagtg gcttgatgac
    67141 cggtaattaa tgccatgcta ccaatatatt cttcttctgt aatagctgaa ctatggtcgc
    67201 gtggaatcca gggcgcaact attttaccgg tataatgcac cgcaataatc gtaaacagta
    67261 aactgacagg aaccacgaac aggttcgaca gtggtgattg ccagaccatg atgcaggcat
    67321 gctgcaacag gatgccaata agaccaaaaa aaccagccag caagcagaga acgacgagag
    67381 ctggcaatct tccgatattg agataatgaa gtgcctggct aatatgacct gtggttatgg
    67441 aatcataatg gtcaagatgt gcatcaagag caccggagag catgtgaccg cagataagcg
    67501 ctaaaatttc taaaaggccg atcaacagta caaaggaaat agcgaaaaga taaggggtat
    67561 tatagtcggc gaataaaatc atatgtcact ccaatgtcca tttagtgacg caatatttac
    67621 tttgggatag tttccttgct tacatttatc tttgttaata ataacacatg ttagagaagt
    67681 tctggaagag aaagtttttt aatatggttt tttatttttc actaaaaagt gtgatcgggg
    67741 acaatatatt tacgcacgtt atgtttaaag gcactacact gattggggaa tactgaaatc
    67801 agaaaaagca tatggcctta aaaggtatgc gataaccgga ggatgtgctt atggatcata
    67861 gtcttaattc tttaaataat ttcgatttcc tggcgcgtag ttttgccaga atgcacgcag
    67921 aaggtcgccc ggtcgatatt cttgccgtta ctggtaacat ggatgaagaa catagaacct
    67981 ggttttgcgc acgttatgcc tggtattgtc aacagatgat gcaggcaaga gagctggagt
    68041 tagagcactg atataacggg cctgatggcc cgttttagtg tttgtattag atgctagtta
    68101 taggtgattt tgaggatgac gtctttacta aacggtccct caacaacagt ctctgccgga
    68161 ttcgcactaa gttctgccct gaatggtgcg gtgaccgaag gtgcactaga cgatgaaaaa
    68221 gaagcaaatt gtgagaagcg gttaaattcc gttgctgttc catctttatg aaatatcttc
    68281 aatagcagtc cattacccat atccagatgg ctataatcac tcaaaatatc cgtagtgaaa
    68341 aagctcccta aaatattgaa ttgctcagta caggccgcac caggatcttt tgtcatgact
    68401 acactaaacg tctcactaac gctggtattt ttaattgttc gggaatttat tttaccaaag
    68461 tcaacaacgc catcagctgg ttccagtgcg acattaacaa agcagtcaag gacatgaata
    68521 ttatttaatc cacgaatcct gaaggtcaaa tttttgtcta cttctatagc tcctgcgcca
    68581 ctcatacctt taaactctag agcgatatat tcatcaggta acaccatgtt tttattaact
    68641 ctattggcat tttttgctaa tttaatatag agttgtacgc gagctggaaa ggttactgat
    68701 aattgaggct caggacctcc actacagaca gcggggtttt tatagtgatt attaaaggta
    68761 cccgctcctt ttatatcaat gcattctggt gctgcgatag cattaggggc agtcgaatca
    68821 taatcaactc cattgtatgt tacgccaaaa atataggagt tctgattata aaagtcctga
    68881 ccgcctggta aaaacatcat tatattagtc caggctgatg ggtattcttc tccaggtgat
    68941 ggggcaggta atgcattatt acagaagaca ttcataaaat actcactgct ttgccagatc
    69001 ttatcacctt ttttaccatt actggggata gaaattcttg gtacgtaacc aatgactacg
    69061 gtatttcccc cttcatgttc agcatagcag tccattgccc atacaggtaa tgcactaaaa
    69121 cctataataa atataattac aattaataag tagcgcatat tatttatctc gtttggctac
    69181 ggaaacatta caagagttat taaccgtcgg gcagtttatt tcataaaaac ttaatccacc
    69241 aaaatcattg atataaccaa cctgaaaagc tgacggaaca tttttaaact gcgtatctgc
    69301 atggctataa ggggttacca ttaaactttg cgtttgtggc aacattgtga ttccgtttgt
    69361 tccgatataa cccagcgtaa cgtgataagg catcttattg ttaaagcgga tcgaaccatc
    69421 atttctacgg atgatttcaa ctttttcgta agacattacc ccatcatctt ctaattcaat
    69481 agcttttggt cgccagaata gtttcaatgc gtgctgcata gtgacttgca tcacattttt
    69541 tttatctgtt tttggcggta tttctcgcac gttaaaatag aagagcgttt ctctgtcatt
    69601 aggcagttga gcaatttttt cctgacttat cattcttatt tgaatttgct cactgggatt
    69661 taaacgtacc atagggggaa ccgtgacaat aaagtcacgt gatttattgc ctttcgtatc
    69721 ttctatccat gtttgcgcca gatagggtag atttttgctt tgattagtaa gttttaatgc
    69781 ttccccttta tctgactcat tcattatgat acgggtacgg tccgggatga tggcacccca
    69841 ggcaatgctg ctcatgcagg cgagtaatac tcctgctgca atgagattct ttgtgtttaa
    69901 gtagcgcatt agtttcatcc tgaattaatt ttggctaata cacggtaaaa taaccgttcc
    69961 ggtggttaag ttgctgctat tgggtggttg aataaaacag gatttatcac cccagcgtaa
    70021 agtaagtttg ctgtcatcct gaatgccgtt gaggtataca aagccgccat cagcaaccag
    70081 tcctaattct ttatggctgg ttttatcttt tacgctaatc cccagtggtg ggtgactacc
    70141 atctgccagg cgaataaccc ccatgatttg atagcccttg cgggtatcaa gtttggcgta
    70201 gccaatagca ccttcggtta aggtggtggt gataaccgag ttttcgatat ccacattttc
    70261 tggaagattt cgggtatcaa cgcttaatga tgttgttatg taactgctga ctgatggaat
    70321 aactccgatg ccataccgat tagttaccgc gcgtttattg ttcaacggaa tatcttctgc
    70381 accatctgcg tcgatcataa aacgcgagtc agcactacca ctatagtcat ggaaagcggc
    70441 tccgtgacga gtcgccgtga atgaaccatt ccaactggca ttcagagaat aataactgtc
    70501 tcgctgtgat gtcccggaga gatagagacg accgttctcg gtattatgct gataactggc
    70561 gcgtagtgac gctttcatat ctttgaattc atcattgtcg cctgaagcag aaacgctcca
    70621 ggaattattt cgatcggaag agtcatacca cgaagcagta tgcgaaatct tgttatcctg
    70681 atttcgttgc ataccatagc tcaatgttct actggttccc cagggaatcg aaatatttag
    70741 gtacagttga tcctggttgt cttcttccca gtttattcgg ctaaatgtta gagaagtgga
    70801 gacgtttttt aatggcccga tatcaaaatc gcggctaagt gaaaatgaat aattcacatt
    70861 actgctggcg tcccagtaag tattacgagc caaacttacg tatgtattta accttaatga
    70921 ttcaagatac tggttaaacg tgacaatata actctctttt tcattctgat aattcgtgta
    70981 atggttagtg tcattgatat attcattcat tgtcacaaag tttttatcag agaaacggta
    71041 accagcgaaa gtgagctggc taccggtaga ttcaaaacgt tttgaatagt tagcgcggta
    71101 gctataaccc gtttctttat cctgattatg caactgagca tcagatcgtg tgacatcaaa
    71161 agataatgaa cccaatgagt taaggttaaa gccaacgccg gcagccagag attgataatc
    71221 acggtttgtt aaaactgaac caccatacag ggatacattg ttcagccaac cccatgagaa
    71281 ttcaccagtc cagaagaagg gttgttgtaa ggaatcacca ccaacgctgg tcggtttacc
    71341 catagcagtg gtgtagcgaa tctgcccttt acgcgtcaga taaggaatac tattagatgc
    71401 aacttgccat tgcgtgactt gcccgtcctc ttcttcaacc ttgacgtcca gttgcccctg
    71461 taatgtattg aataaattag taatagcaaa tgggccagga ggaacatttt cttgataaat
    71521 aatacggttg ttctgtgaga cagtgacctt tgcattcgtt tgtgcaacac ccgtgatttg
    71581 cggtgcgtaa ccacgtagat caggcggcaa catattctca tcgcttttca acgaaacgcc
    71641 agaaaaatgg aaagagtcga aaatagagga ttcagtatcg tattgaccta tagttagttt
    71701 tgcattaatt gctgggattg ggcgaaacaa ataaatacgc ggaaaatcca ggttgttcgc
    71761 ctggcttttg ccattgttaa atgattgatc gtactggtaa tcagtacgca ggcgccaggc
    71821 cccaagatta accccagcct gcccatagga acttatattt tgtgaattat cgccctgatg
    71881 tggtgcataa tgagaggcat ataaattgta gtcaagaaat gccccagcaa taccatgatc
    71941 ccaaagttca ggaggcgtcc agtttgcatc tttgtatttt aaccatgcct gaggtgcaga
    72001 tatggataat tgcattttcc ctttatcgag ataagttgta atttgttttt ctttttcgat
    72061 agggtaacaa ccatcgatca ggttttgctg agccacttca ataatctcat cagtaaagcc
    72121 taataatgtt aattgctctg gagtcagaca aactgcagtt tggttatcaa ttaataccca
    72181 ttgcaggtcc agatggattg gcgtcctttt attattaata agagcgttta atgagtattt
    72241 acctacgggt aattggccat cagtttcaaa ttgggaaagg ttaacgtttt cacgatcttc
    72301 ggcttcgatt aaatctttgt tgaattcaac ggcataagct gacgcacaac caatgatgag
    72361 aacggataat gaacaataaa tatttttaat caattcgctt attgtggtta attttaattt
    72421 tttatacatc tgttccactt atcattaatc cgttaatgat gtggccgggc gaacccggcc
    72481 taatgtgatt actcgtaagc aatcttatag tcagcctgag cgtaaacgtt gcccggggta
    72541 gcatctttgc ttggggattc catacgagct ttgaagttca gttcctgata gctgttagta
    72601 ttgttgaatg taattactac cggagtacct aaagtcactt cgccgctctg gctatccaga
    72661 atacgtacgc caacgcctgt ggcaccaccg atttcagttt ctttggtgtt tttcagcagg
    72721 tcagtctgac ccgtcatagt attcgctgtg gtgaaagtaa ccttcgcttt tgaataagtg
    72781 gttccaccct ggctcaacat gcagtcttgc aggtgaatag tgaaaggctc agccaaagat
    72841 ttctggtcgc ttttcagaac ggtatctgct acttcaccca gatttacagt taagtcttcg
    72901 tcacctggtt taattgaaca tggagcagag ataacttcac ctttaaattt aacaatgccg
    72961 tgaccttcgt cagcaaatac gttattggaa agaatcattg ctacgccagc aacaagaagt
    73021 gactttttaa aagcagacat a
//
LOCUS       DAZFQD010000006        72869 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.006, whole genome shotgun sequence.
ACCESSION   DAZFQD010000006 DAZFQD010000000
VERSION     DAZFQD010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 72869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 72869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..72869
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.006"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            65..637
                     /gene="lpfA-O113"
                     /locus_tag="ACHKTW_000422"
     CDS             65..637
                     /gene="lpfA-O113"
                     /locus_tag="ACHKTW_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbria major subunit LpfA-O113"
                     /protein_id="HFO6461506.1"
                     /translation="MKRNIIGGAFTLASLMLAGHALAEDGVVHFVGEIVDTTCEVTSD
                     TADQIVPLGKVSKNAFSGVGSLASPQKFSIKLENCPATYTQAAVRFDGTEAPGGDGDL
                     KVGTPLTAGNPGDFTGTGQAIAATGVGIRIFNQSDNSQVKLYNDSAYTAIDAEGKAEM
                     KFIARYVATNATVTAGTANADSQFTVEYKK"
     gene            685..1416
                     /gene="lpfB-O113"
                     /locus_tag="ACHKTW_000423"
     CDS             685..1416
                     /gene="lpfB-O113"
                     /locus_tag="ACHKTW_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_316045823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial chaperone LpfB-O113"
                     /protein_id="HFO6461507.1"
                     /translation="MSRKSCLIRLFKTTVSCKLFTAIILSVFLGQPALTYAGVVIGGT
                     RVVYLSNNADKSISVFSKEEKIPYLIQAWVDPFNKEDKSKAPFTVIPPVSRLEPSQEK
                     ILRIIHTKGVSLPDDRESVFWLNIKNIPPSASNKATNSLEIAVKTRIKLFWRPANIRL
                     IPEDAAPKVKWRREGRNLIAENPNPIHISVMDVIVDGHDVPLNMIRPFETLTLPLPAN
                     SAGGQMTWRFINDYGAVSDPIKMTL"
     gene            1441..3963
                     /gene="lpfC-O113"
                     /locus_tag="ACHKTW_000424"
     CDS             1441..3963
                     /gene="lpfC-O113"
                     /locus_tag="ACHKTW_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial usher protein LpfC-O113"
                     /protein_id="HFO6461508.1"
                     /translation="MMTTRIVVGLTAGTCLIFSQNLMAEVSVFNPALLEIDHQSGVDI
                     RQFNRANLMPPGVYSVDIFINGKMFERQDVTFVQDNPDADLHACFIAIKKTLSSFGIK
                     VDALKSFNDVDETVCLDPAPRIEGSSWQFDSDKLQLNISIPQIYMDAMAYDYISPTRW
                     DEGINALTINYDFSGSHTLRSDYGSQETDTSYLNLRNGLNIGPWRLRNYSTLNTSDGR
                     AEYNSISTWIQRDIAALRSQIMIGDTWTASDIFDSTQIRGARLYTDNDMLPASQNGFA
                     PVVRGIAKSNATVIIRQNGYVIYQSAVPQGAFEITDLNTASTGGDLDVTIKEEDGSEQ
                     RFTQPYASLAILKREGQTDVDVSVGELRDEDGFTPDVLQAQILHGFSHGITLYGGMQA
                     AENYGSAALGVGKDLGALGAISFDVTHARANFSHDDTETGQSYRFLYSKRFDDTDTSL
                     RLVGYRYSTEGYYTLNEWASRRNSPEDFWETGNRRSRVEGTLTQSLGRDYGNLYLTLS
                     RQQYWHTDDVERLMQFGYSSSWKRLSWNVSWSYSNTARQGTGNNHASDNTSEQIYMLS
                     LSVPLSGWWGNSYATYSVSQNDNSGSSHQLGLSGTALERNNLSWNLMQSYNSHDDEVG
                     GNMSLTYDGSYGTVNGSYNYSQNSQRLNYGIRGGILAHSEGVTLSQELGETIALVKAP
                     GAAGLEIDNMRGAATDWRGYTVKTQLNPYDENRVAISDNYFSKSNIELDNTVVTMVPT
                     RGAVVKAEFVTHVGYRVLFRVLNANGKPVPFGAIAAIQDASLADSGIVGDRGELYLSG
                     LPEKGQVTLSWGENASTKCIFNYSLSTPESESGLIEQGVTCH"
     gene            3974..5047
                     /gene="lpfD-O113"
                     /locus_tag="ACHKTW_000425"
     CDS             3974..5047
                     /gene="lpfD-O113"
                     /locus_tag="ACHKTW_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="LpfA-O113 is the major subunit of a form of long
                     polar fimbria first described in LEE-negative, Shiga
                     toxin-producing enterohemorrhagic Escherichia coli (STEC)
                     of serotype O113:H21. This protein, designated LpfD-O113,
                     is the minor subunit of this chaperone-usher type fimbrial
                     system; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial minor subunit LpfD-O113"
                     /protein_id="HFO6461509.1"
                     /translation="MNKYIKQWCFAVFMLSLSSVALAAPKGICTPDNGVFHSTLDFSG
                     YLITANENKVGTTFNTTVTNGSSYPGRCHCDTGNVGEFPYIYYTSKINQALTYAGVHS
                     NINYYDLNPNLDVGIAIDILGVGYVNAPFEYHANNPSGNTKYNCNRIEPLSISSGAKA
                     IVYFYIKKTFAGKLIIPETKIVTLYGTISRDTPVDYSQPMADVYIRGDITAPQSCEIN
                     NLQPVYFDFKEIPAADFSSVVGSAVTTHKITKTVTIECENLGILNTDDISTSFYATEP
                     NTDNSMVVTSNSNVGIKIYDKNNKEIKVNGGELPTDMGKSTVYGEKSGSVTFSAAPAS
                     LTGARPAPGQFTATATITVEIVR"
     gene            5295..6335
                     /gene="pstS"
                     /locus_tag="ACHKTW_000426"
     CDS             5295..6335
                     /gene="pstS"
                     /locus_tag="ACHKTW_000426"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HFO6461510.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            6422..7381
                     /gene="pstC"
                     /locus_tag="ACHKTW_000427"
     CDS             6422..7381
                     /gene="pstC"
                     /locus_tag="ACHKTW_000427"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HFO6461511.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            7381..8271
                     /gene="pstA"
                     /locus_tag="ACHKTW_000428"
     CDS             7381..8271
                     /gene="pstA"
                     /locus_tag="ACHKTW_000428"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFO6461512.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            8362..9135
                     /gene="pstB"
                     /locus_tag="ACHKTW_000429"
     CDS             8362..9135
                     /gene="pstB"
                     /locus_tag="ACHKTW_000429"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFO6461513.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            9150..9875
                     /gene="phoU"
                     /locus_tag="ACHKTW_000430"
     CDS             9150..9875
                     /gene="phoU"
                     /locus_tag="ACHKTW_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFO6461514.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGKDSDK"
     gene            10161..10997
                     /gene="bglG"
                     /locus_tag="ACHKTW_000431"
     CDS             10161..10997
                     /gene="bglG"
                     /locus_tag="ACHKTW_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HFO6461515.1"
                     /translation="MNMQITKILNNNVVVVIDDQQREKVVMGRGIGFQKRAGERINSS
                     GIEKEYALSSHELNGRLSELLSHIPLEVMATCDRIISLAQERLGKLQDSIYISLTDHC
                     QFAIKRFQQNVLLPNPLLWDIQRLYPKEFQLGEEALTIIDKRLGVQLPKDEVGFIAMH
                     LVSAQMSGNMEDVAGVTQLMREMLQLIKFQFSLNYQEESLSYQRLVTHLKFLSWRILE
                     HASINDSDESLQQAVKQNYPQAWQCAERIAIFIGLQYQRKISPAEIMFLAINIERVRK
                     EH"
     gene            11130..13007
                     /gene="bglF"
                     /locus_tag="ACHKTW_000432"
     CDS             11130..13007
                     /gene="bglF"
                     /locus_tag="ACHKTW_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
                     /protein_id="HFO6461516.1"
                     /translation="MTELARKIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLK
                     NTPGIIMVVESGGQFQVVIGNHVADVFLAVNSVAGLGEKAQQAPENDEKGNLLNRFVY
                     VISGIFTPLIGLMAATGILKGMLALALTFQWTTEQSGTYLILFSASDALFWFFPIILG
                     YTAGKRFGGNPFTAMVIGGALVHPLILTAFENGQKADELGLDFLGIPVTLLNYSSSVI
                     PIIFSAWLCSILERRLNAWLPSAIKNFFTPLLCLMVITPVTFLLVGPLSTWISELIAA
                     GYLWLYQAVPAFAGAVMGGFWQIFVMFGLHWGLVPLCINNFTVLGYDTMIPLLMPAIM
                     AQVGAALGVFLCERDAQKKVVAGSAALTGLFGITEPAVYGVNLPRKYPFVIACISGAL
                     GATIIGYAQTKVYSFGLPSIFTFMQTIPSTGIDFTVWASVIGGVIAIGCAFVGTVMLH
                     FITAKRQPAQGAPQEKTPEVITPPEQGGICSPMTGEIVPLIHVADTTFASGLLGKGIA
                     ILPSVGEVRSPVAGRIASLFATLHAIGIESDDGVEILIHVGIDTVKLDGKFFSAHVNV
                     GDKVNTGDRLISFDIPAIREAGFDLTTPVLISNSDDFTDVLPHGTAQISAGEPLLSII
                     R"
     gene            13026..14420
                     /gene="bglB"
                     /locus_tag="ACHKTW_000433"
     CDS             13026..14420
                     /gene="bglB"
                     /locus_tag="ACHKTW_000433"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglB"
                     /protein_id="HFO6461517.1"
                     /translation="MKAFPETFLWGGATAANQVEGAWLEDGKGITTSDLQPHGVMGKM
                     EPRILGKENIKDVAIDFYHRYPEDIALFAEMGFTCLRISIAWARIFPQGDEAEPNEAG
                     LAFYDRLFDEMAQAGIKPLVTLSHYEMPYGLVKNYGGWANRAVIDHFEHYARTVFTRY
                     QHKVALWLTFNEINMSLHAPFTGVGLAEESGEAEVYQAIHHQLVASARAVKACHSLIP
                     EAKIGNMLLGGLVYPLTCQPQDMLEAMEENRRWMFFGDVQARGQYPGYMQRFFRDHNI
                     TIEMTESDAEDLKHTVDFISFSYYMTGCVSHDESINKNAQGNILNMIPNPHLKSSEWG
                     WQIDPVGLRVLLNTLWDRYQKPLFIVENGLGAKDSVEADGSIQDDYRIAYLNDHLVQV
                     NEAIADGVDIMGYTSWGPIDLVSASHSQMSKRYGFIYVDRDDNGEGSLTRTRKKSFGW
                     YAEVIKTRGLSLKK"
     gene            14506..16122
                     /gene="bglH"
                     /locus_tag="ACHKTW_000434"
     CDS             14506..16122
                     /gene="bglH"
                     /locus_tag="ACHKTW_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-specific porin BglH"
                     /protein_id="HFO6461518.1"
                     /translation="MFRRNLITSAILLMAPLAFSAQSLAESLTVEHRLELLEKALRET
                     QSELKKYKDEEKKKYTPATVNRSVSTNDQGYAANPFPTSSAAKPDAVLVKNEEKNASE
                     TGSIYSSMTLKDFSKFVKDEIGFSYNGYYRSGWGTASHGSPKSWAIGSLGRFGNEYSG
                     WFDLQLKQRVYNENGKRVDAVVMMDGNVGQQYSTGWFGDNAGGENFMQFSDMYVTTKG
                     FLPFAPEADFWVGKHGAPKIEIQMLDWKTQRTDAAAGVGLENWKVGPGKIDIALVRED
                     VDDYDRSLQNKQQINTNTIDLRYKDIPLWDKATLMVSGRYVTANESASEKDNQDNNGY
                     YDWKDTWMFGTSLTQKFDKGGFNEFSFLVANNSIASNFGRYAGASPFTTFNGRYYGDH
                     TGGTAVRLTSQGEAYIGNHFIVANAIVYSFGNDIYSYETGAHSDFESIRAVVRPAYIW
                     DQYNQTGVELGYFTQQNKDANSNKFNESGYKTTLFHTFKVNTSMLTSRPEIRFYATYI
                     KALENELDGFTFEDNKDDQFAVGAQAEIWW"
     gene            16149..17318
                     /gene="yieL"
                     /locus_tag="ACHKTW_000435"
     CDS             16149..17318
                     /gene="yieL"
                     /locus_tag="ACHKTW_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418175.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFO6461519.1"
                     /translation="MNIKIAALTLAIASGISAQWAIAADMPASPAPTIPVKQYVTQVN
                     ADNSVTFRYFAPGAKNVSVVVGVPVLDNIHPMTKDEAGVWSWRTPILKGNLYEYFFNV
                     DGVRSIDTGTAMTKPQRQVNSSMILVPGSYLDTRSVAHGDLIAITYHSNALQSERQMY
                     VWTPPGYTGMGEPLPVLYFYHGFGDTGRSAIDQGRIPQIMDNLLAEGKIKPMLVVIPD
                     TETDAKGIIPEDFVPQERRKVFYPLNAKAADRELMNDIIPLISKRFNVSKDADGRALA
                     GLSQGGYQALVSGMNHLESFGWLATFSGVTTTTVPDEGVAARLNDPAAINQQLRNFTV
                     VVGDKDVVTGKDIAGLKTELEQKKIKFDYQEYPGLNHEMDVWRPAYAAFVQKLFK"
     gene            17333..18055
                     /gene="yieK"
                     /locus_tag="ACHKTW_000436"
     CDS             17333..18055
                     /gene="yieK"
                     /locus_tag="ACHKTW_000436"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFO6461520.1"
                     /translation="MKLIITEDYQEMSRVAAHHLLGYMSKTRRVNLAITAGSTPKGMY
                     EYLTTLVKGKPWYDNCYFYNFDEIPFRGKEGEGVTITNLRNLFFTPAGIKEENIQKLT
                     IDNYREHDQKLAREGGLDLVVLGLGADGHFCGNLPNTTHFHEQTVEFPIQGEMVDIVA
                     HGELGGDFSLVPDSYVTMGPKSIMAAKNLLIIVSGAGKAQALKNVLQGPVTEDVPASV
                     LQLHPSLMVIADKAAAAELALG"
     gene            complement(18117..18704)
                     /gene="cbrC"
                     /locus_tag="ACHKTW_000437"
     CDS             complement(18117..18704)
                     /gene="cbrC"
                     /locus_tag="ACHKTW_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PF03691 family colicin E2 tolerance protein
                     CbrC"
                     /protein_id="HFO6461521.1"
                     /translation="MTQNIRPLPQFKYHPKPLETGAFEQDKTVECDCCEQQTSVYYSG
                     PFYCVDEVEHLCPWCIADGSAAEKFAGSFQDDASIEGVEFEYDEEDEFAGIKNTYPDE
                     MLKELVERTPGYHGWQQEFWLAHCGDFCAFIGYVGWNDIKDRLDEFANLEEDCENFGI
                     RNSDLAKCLQKGGDCQGYLFRCLHCGKLRLWGDFS"
     gene            complement(18753..19226)
                     /locus_tag="ACHKTW_000438"
     CDS             complement(18753..19226)
                     /locus_tag="ACHKTW_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6461522.1"
                     /translation="MSVSRRVIHHGLYFAVLGPLIGVLFLVLYIFFAKEPLVLLRRIQ
                     VLPLFLLLSITTGVIPALLTGVMVACLPEKIGSQKNYRCLAGGIGGVVITEIYCAVIV
                     HIKGMASSELFENILSGDSLVVRIIPALLAGVVMSRIITRLPGLDISCPETDSLS"
     gene            complement(19293..19958)
                     /gene="yieH"
                     /locus_tag="ACHKTW_000439"
     CDS             complement(19293..19958)
                     /gene="yieH"
                     /locus_tag="ACHKTW_000439"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HFO6461523.1"
                     /translation="MSQIEAVFFDCDGTLVDSEVICSRAYVTMFQEFGITLDPEEVFK
                     RFKGVKLYEIIDIVSLEHGVTLAKTEAEHVYRAEVARLFDSELEAIEGAGALLSAITA
                     PMCVVSNGPNNKMQHSMGKLNMLHYFPDKLFSGYDIQRWKPDPALMFHAAKAMNVNVE
                     NCILVDDSVAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTHLSQLPELWKARGWD
                     ITA"
     gene            20125..21462
                     /gene="adeP"
                     /locus_tag="ACHKTW_000440"
     CDS             20125..21462
                     /gene="adeP"
                     /locus_tag="ACHKTW_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HFO6461524.1"
                     /translation="MSQQHTTQASGQGMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQVGMGAIFWGAIGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANPETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
     gene            complement(21516..22082)
                     /gene="yieF"
                     /locus_tag="ACHKTW_000441"
                     /gene_synonym="chrR"
     CDS             complement(21516..22082)
                     /gene="yieF"
                     /locus_tag="ACHKTW_000441"
                     /gene_synonym="chrR"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HFO6461525.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEEGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDPQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(22104..22853)
                     /gene="yieE"
                     /locus_tag="ACHKTW_000442"
     CDS             complement(22104..22853)
                     /gene="yieE"
                     /locus_tag="ACHKTW_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HFO6461526.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPTIEKLSVWEFDGKHGWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKAFSPN"
     gene            complement(23010..23969)
                     /gene="yidZ"
                     /locus_tag="ACHKTW_000443"
     CDS             complement(23010..23969)
                     /gene="yidZ"
                     /locus_tag="ACHKTW_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HFO6461527.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTPLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHETWNLDTFLRYPHISICWEQSDTWAL
                     DNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQYYNQLHQLPLVALPL
                     PFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRETIKNLYASMA"
     gene            complement(23944..25118)
                     /gene="mdtL"
                     /locus_tag="ACHKTW_000444"
                     /pseudo
     CDS             complement(23944..25118)
                     /gene="mdtL"
                     /locus_tag="ACHKTW_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418166.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
     gene            complement(25250..26497)
                     /gene="tnaB"
                     /locus_tag="ACHKTW_000445"
     CDS             complement(25250..26497)
                     /gene="tnaB"
                     /locus_tag="ACHKTW_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
                     /protein_id="HFO6461528.1"
                     /translation="MTDQAEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAF
                     ILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTY
                     AYISANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLASSRITSLFLGLKI
                     ISFVIVFGSFFFQVDYSILRDATSTTAGTSYFPYIFMALPVCLASFGFHGNIPSLIIC
                     YGKRKDKLIKSVVFGSLLALVIYLFWLYCTMGNIPRESFKAIISSGGNVDSLVKSFLG
                     TKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHGGRFKTVLLTFLPP
                     ALLYLIFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIPAIV
                     ILFGITVILCWFGNVFNVLPKFG"
     gene            complement(26588..28003)
                     /gene="tnaA"
                     /locus_tag="ACHKTW_000446"
     CDS             complement(26588..28003)
                     /gene="tnaA"
                     /locus_tag="ACHKTW_000446"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009034 - tryptophanase activity [Evidence
                     IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HFO6461529.1"
                     /translation="MENFKHLPEPFRIRVIEPVKRTTRAYREEAIIKSGMNPFLLDSE
                     DVFIDLLTDSGTGAVTQSMQAAMMRGDEAYSGSRSYYALAESVKNIFGYQYTIPTHQG
                     RGAEQIYIPVLIKKREQEKGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFD
                     TGVRYDFKGNFDLEGLERGIEEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAK
                     KYDIPVVMDSARFAENAYFIKQREAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPM
                     GGLLCMKDDSFFDVYTECRTLCVVQEGFPTYGGLEGGAMERLAVGLYDGMNLDWLAYR
                     IAQVQYLVDGLEEIGVVCQQAGGHAAFVDAGKLLPHIPADQFPAQALACELYKVAGIR
                     AVEIGSFLLGRDPKTGKQLPCPAELLRLTIPRATYTQTHMDFIIEAFKHVKENAANIK
                     GLTFTYEPKVLRHFTAKLKEV"
     gene            complement(28224..28298)
                     /gene="tnaC"
                     /locus_tag="ACHKTW_000447"
     CDS             complement(28224..28298)
                     /gene="tnaC"
                     /locus_tag="ACHKTW_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006569 - tryptophan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0031554 - regulation of termination of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase leader peptide"
                     /protein_id="HFO6461530.1"
                     /translation="MNILHICVTSKWFNIDNKIVDHRP"
     gene            complement(28541..29905)
                     /gene="mnmE"
                     /locus_tag="ACHKTW_000448"
     CDS             complement(28541..29905)
                     /gene="mnmE"
                     /locus_tag="ACHKTW_000448"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFO6461531.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGFKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVIADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALAQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
     gene            complement(30011..31657)
                     /gene="yidC"
                     /locus_tag="ACHKTW_000449"
     CDS             complement(30011..31657)
                     /gene="yidC"
                     /locus_tag="ACHKTW_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFO6461532.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISTFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIAAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            complement(31660..31917)
                     /gene="yidD"
                     /locus_tag="ACHKTW_000450"
     CDS             complement(31660..31917)
                     /gene="yidD"
                     /locus_tag="ACHKTW_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFO6461533.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            complement(31881..32240)
                     /gene="rnpA"
                     /locus_tag="ACHKTW_000451"
     CDS             complement(31881..32240)
                     /gene="rnpA"
                     /locus_tag="ACHKTW_000451"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFO6461534.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            complement(32257..32397)
                     /gene="rpmH"
                     /locus_tag="ACHKTW_000452"
     CDS             complement(32257..32397)
                     /gene="rpmH"
                     /locus_tag="ACHKTW_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFO6461535.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            complement(32627..32707)
                     /locus_tag="ACHKTW_000453"
     CDS             complement(32627..32707)
                     /locus_tag="ACHKTW_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6461536.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTHQPV"
     gene            33004..34407
                     /gene="dnaA"
                     /locus_tag="ACHKTW_000454"
     CDS             33004..34407
                     /gene="dnaA"
                     /locus_tag="ACHKTW_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFO6461537.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAARQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            34412..35512
                     /gene="dnaN"
                     /locus_tag="ACHKTW_000455"
     CDS             34412..35512
                     /gene="dnaN"
                     /locus_tag="ACHKTW_000455"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFO6461538.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNPLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            35512..36585
                     /gene="recF"
                     /locus_tag="ACHKTW_000456"
     CDS             35512..36585
                     /gene="recF"
                     /locus_tag="ACHKTW_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFO6461539.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMADTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            36614..39028
                     /gene="gyrB"
                     /locus_tag="ACHKTW_000457"
     CDS             36614..39028
                     /gene="gyrB"
                     /locus_tag="ACHKTW_000457"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFO6461540.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            39268..39666
                     /gene="yidB"
                     /locus_tag="ACHKTW_000458"
     CDS             39268..39666
                     /gene="yidB"
                     /locus_tag="ACHKTW_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418153.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HFO6461541.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQGNQSVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSPQANNDLLSAGMELLKGKLFR"
     gene            39781..40593
                     /gene="yidA"
                     /locus_tag="ACHKTW_000459"
     CDS             39781..40593
                     /gene="yidA"
                     /locus_tag="ACHKTW_000459"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HFO6461542.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(40639..41295)
                     /gene="yidX"
                     /locus_tag="ACHKTW_000460"
     CDS             complement(40639..41295)
                     /gene="yidX"
                     /locus_tag="ACHKTW_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YidX"
                     /protein_id="HFO6461543.1"
                     /translation="MKLNFKGFFKAAGLFPLALMLSGCISYALVSHTAKGSSGKYQSQ
                     SDTITGLSQAKDSNGTKGYVFVGESLDYLITDGADDIVKMLNDPALNRHNIQVADDAR
                     FVLNAGKKKFTGTISLYYYWNNEEEKALATHYGFACGVQHCTRSLENLKGTIHEKNKN
                     MDYSKVMAFYHPFKVRFYEYYSPRGIPDGVSAALLPVTVTLDIITAPLQFLVVYAVNQ
                     "
     gene            41573..42262
                     /gene="dgoR"
                     /locus_tag="ACHKTW_000461"
     CDS             41573..42262
                     /gene="dgoR"
                     /locus_tag="ACHKTW_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HFO6461544.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLPAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            42259..43137
                     /gene="dgoK"
                     /locus_tag="ACHKTW_000462"
     CDS             42259..43137
                     /gene="dgoK"
                     /locus_tag="ACHKTW_000462"
                     /EC_number="2.7.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HFO6461545.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHCLDSRQSEAGVTRLNGKSPA
                     AVLAEVTTDWREENTPVVMAGMVGSNVGWKVAPYLSVPARFSSIGDQLTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARTLAPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     GELHHLLLNHSLIGAGLPPQENSADAFAAGLERGLNAPAILPQLFEVRASHVLGTLPR
                     EQVSEFLSGLLIGAEVASMRDYVAHQHAITLVAGTSLTARYQQAFQAMGCDVTAVAGD
                     TAFQAGIRSIAHAVAN"
     gene            43121..43738
                     /gene="dgoA"
                     /locus_tag="ACHKTW_000463"
     CDS             43121..43738
                     /gene="dgoA"
                     /locus_tag="ACHKTW_000463"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HFO6461546.1"
                     /translation="MQWQTKLPLIAILRGIKPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGAGTVLKPEQVDALARMGCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALEAGAQALKIFPSSAFGPQYIKALKAVLPSDIAVFAVGGVTPE
                     NLAQWIDAGCAGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            43735..44883
                     /gene="dgoD"
                     /locus_tag="ACHKTW_000464"
     CDS             43735..44883
                     /gene="dgoD"
                     /locus_tag="ACHKTW_000464"
                     /EC_number="4.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008869 - galactonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
                     /protein_id="HFO6461547.1"
                     /translation="MKITKITTYRLPPRWMFLKIETDEGVVGWGEPVIEGRARTVEAA
                     VHELGDYLIGQDPSRINDLWQVMYRAGFYRGGPILMSAIAGIDQALWDIKGKVLNAPV
                     WQLMGGLVRDKIKAYSWVGGDRPADVIDGIKTLREIGFDTFKLNGCEELGLIDNSRAV
                     DAAVNTVAQIREAFGNQIEFGLDFHGRVSAPMAKVLIKELEPYRPLFIEEPVLAEQAE
                     YYPKLAAQTHIPLAAGERMFSRFDFKRVLEAGGISILQPDLSHAGGITECYKIAGMAE
                     AYDVTLAPHCPLGPIALAACLHIDFVSYNAVLQEQSMGIHYNKGAELLDFVKNKEDFS
                     MVGGFFKPLTKPGLGVEIDEAKVIEFSKNAPDWRNPLWRHVDNSVAEW"
     gene            44958..46295
                     /gene="dgoT"
                     /locus_tag="ACHKTW_000465"
     CDS             44958..46295
                     /gene="dgoT"
                     /locus_tag="ACHKTW_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO6461548.1"
                     /translation="MVSGFAMPKIWIKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAEMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYYIA
                     IFGWSVATLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASTLWFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFVGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(46292..47356)
                     /gene="cbrA"
                     /locus_tag="ACHKTW_000466"
     CDS             complement(46292..47356)
                     /gene="cbrA"
                     /locus_tag="ACHKTW_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance lipid reductase CbrA"
                     /protein_id="HFO6461549.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQCGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPTSVEVYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRHLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNSITNCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTTLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSADILRSVLLKQPEKLNTAYWRATRKLRLKLFGKIVKSRCLTA
                     PALRKWIMRSGVAHIPQLKD"
     gene            47457..48671
                     /gene="yidR"
                     /locus_tag="ACHKTW_000467"
     CDS             47457..48671
                     /gene="yidR"
                     /locus_tag="ACHKTW_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HFO6461550.1"
                     /translation="MKQITFAPRNHLLTNTNTWTPDSQWLVFDVRPSGASFTGETIER
                     VNIHTGEVEVIYRASQGAHVGVVTVHPKSEKYVFIHGPENPDETWHYDFHHRRGVIAE
                     GGKVSNLDAMDITAPYTPGALRGGSHVHVFSPNGERVSFTYNDHVMHELDPALDLRNV
                     GVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAYEEGWVGNHALAF
                     IGDTLSPKGEKVPELFIVELPQDEAGWKAAGDAPLSGTETTLPAPPRGVVQRRLTFTH
                     HRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRQLTHNKTDIQ
                     SAFNWHPSGEWLGFVLDNRIACAHAQSGEVEYLTENHANPPSADAVVFSPDGQWLAWM
                     EGGQLWITETDR"
     gene            complement(48673..49005)
                     /gene="yidQ"
                     /locus_tag="ACHKTW_000468"
     CDS             complement(48673..49005)
                     /gene="yidQ"
                     /locus_tag="ACHKTW_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HFO6461551.1"
                     /translation="MIRNVLLAFMICSGMTLLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     misc_feature    49244..49316
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            49310..49723
                     /gene="ibpA"
                     /locus_tag="ACHKTW_000469"
     CDS             49310..49723
                     /gene="ibpA"
                     /locus_tag="ACHKTW_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpA"
                     /protein_id="HFO6461552.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     gene            49835..50263
                     /gene="ibpB"
                     /locus_tag="ACHKTW_000470"
     CDS             49835..50263
                     /gene="ibpB"
                     /locus_tag="ACHKTW_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpB"
                     /protein_id="HFO6461553.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISECPALNS"
     gene            50460..52121
                     /gene="yidE"
                     /locus_tag="ACHKTW_000471"
     CDS             50460..52121
                     /gene="yidE"
                     /locus_tag="ACHKTW_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HFO6461554.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(52118..52834)
                     /gene="yidP"
                     /locus_tag="ACHKTW_000472"
     CDS             complement(52118..52834)
                     /gene="yidP"
                     /locus_tag="ACHKTW_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFO6461555.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPVIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLTPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEYQVEYHLRRLHPEKS"
     gene            53130..54746
                     /locus_tag="ACHKTW_000473"
     CDS             53130..54746
                     /locus_tag="ACHKTW_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFO6461556.1"
                     /translation="MLSQIQRFGGAMFTPVLLFPFAGIVVGLAILLQNPMFVGESLTD
                     PNSLFAQIVHIIEEGGWTVFRNMPLIFAVGLPIGLAKQAQGRACLAVMVSFLTWNYFI
                     NAMGMTWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTALHNRLFDK
                     KLPVFLGIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQMGIESLQAFLRSAGALGVWV
                     YTFLERILIPTGLHHFIYGPFIFGPAAVEGGIQMYWAQHLQEFSLSAEPLKSLFPEGG
                     FALHGNSKIFGAVGISLAMYFTAAPENRVKVAGLLIPATLTAMLVGITEPLEFTFLFI
                     SPLLFAVHAVLAASMSTVMYLFGVVGNMGGGLIDQVLPQNWIPMFSNHADMMLTQIAI
                     GLCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQTTAAEPKKELDQA
                     AGILQALGGVGNISSINNCATRLRIALHDMSQTLDDEAFKKLGAHGVFRSGDAIQVII
                     GLHVSQLREQLDSLINSHQSAENVAITEAV"
     gene            54746..55560
                     /locus_tag="ACHKTW_000474"
                     /pseudo
     CDS             54746..55560
                     /locus_tag="ACHKTW_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(55557..56450)
                     /gene="yidL"
                     /locus_tag="ACHKTW_000475"
     CDS             complement(55557..56450)
                     /gene="yidL"
                     /locus_tag="ACHKTW_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFO6461557.1"
                     /translation="MNGKLQSSDVKNETPYNIPLLINENVISSGISLISLWHTYADEH
                     YRVIWPRDKKKPLIANSWVAVYTVQGCGKILLKNGEQITLHGNCIIFLKPMDIHSYHC
                     EGLVWEQYWMEFTPTSMMDIPVGQQSVIYNGEIYNQELTEVAELITSPEAIKNNLAVA
                     FLTKIIYQWICLMYADGKKDPQRRQIEKLIATLHASLQQRWSVADMAATIPCSEAWLR
                     RLFLRYTGKTPKEYYLDARLDLALSLLKQQGNSVGEVADTLNFFDSFHFSKAFKHKFG
                     YAPSAVLKNTDQHPTDASPHN"
     gene            56617..58332
                     /gene="yidK"
                     /locus_tag="ACHKTW_000476"
     CDS             56617..58332
                     /gene="yidK"
                     /locus_tag="ACHKTW_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HFO6461558.1"
                     /translation="MNSLQILSFVGFTLLVAVITWWKVRKTDTGSQQGYFLAGRSLKA
                     PVIAASLMLTNLSTEQLVGLSGQAYKSGMSVMGWEVTSAVTLIFLALIFLPRYLKRGI
                     ATIPDFLEERYDKTTRIIIDFCFLIATGVCFLPIVLYSGALALNSLFHVGESLQISHG
                     AAIWLLVILLGLAGILYAVIGGLRAMAVADSINGIGLVIGGLMVPVFGLIAMGKGSFM
                     QGIEQLTTVHAEKLNSIGGPTDPLPIGAAFTGLILVNTFYWCTNQGIVQRTLASKSLA
                     EGQKGALLTAVLKMLDPLVLVLPGLIAFHLYQDLPKADMAYPTLVNNVLPVPMVGFFG
                     AVLFGAVISTFNGFLNSASTLFSMGIYRRIINQNAEPQQLVTVGRKFGFFIAIVSVLV
                     APWIANAPQGLYSWMKQLNGIYNVPLVTIIIMGFFFPRIPALAAKVAMGIGIISYITI
                     NYLVKFDFHFLYVLACTFCINVVVMLVIGFIKPRATPFTFKDAFAVDMKPWRNVKIAS
                     IGILFAMIGVYAGLAEFGGYGTRWLAMISYFIAAVVIVYLIFDSWRHRHDPAVTFTPD
                     AKDSL"
     gene            58329..59822
                     /gene="yidJ"
                     /locus_tag="ACHKTW_000477"
     CDS             58329..59822
                     /gene="yidJ"
                     /locus_tag="ACHKTW_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFO6461559.1"
                     /translation="MKRPNFLFIMTDTQATNMVGCYSGKPLNTQNIDSLAAEGIRFNS
                     AYTCSPVCTPARAGLFTGIYANQSGPWTNNVALGKNISTMGRYFKDAGYHTCYIGKWH
                     LDGHDYFGTGECPPEWDADYWFDGANYLSELTEKEISLWRNGLNSVEDLQANHIDETF
                     TWAHRISNRAVDFLQQPARAEEPFLMVVSYDEPHHPFTCPVEYLEKYADFYYDLGEKA
                     QDDLANKPEHHRLWAQAMPSPVGDDGLYHHPLYFACNDFVDDQIGRVINALTPEQREN
                     TWVIYTSDHGEMMGAHKLISKGAAMYDDITRIPLIIRSPQGERRQVDTPVSHIDLLPT
                     MMALADIEKPEILPGENILAVKEPRGVMVEFNRYEIEHDSFGGFIPVRCWVTDDFKLV
                     LNLFTSDELYDRRNDPNEMHNLIDDIRFADVRSKMHDALLDYMDKIRDPFRSYQWSLR
                     PWRKDALPRWMGAFRPRPQDGYSPVVRDYDTGLPTQGVKVEEKKQKF"
     gene            complement(59869..60318)
                     /gene="yidI"
                     /locus_tag="ACHKTW_000478"
     CDS             complement(59869..60318)
                     /gene="yidI"
                     /locus_tag="ACHKTW_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YidI"
                     /protein_id="HFO6461560.1"
                     /translation="MGIIAQNKISSLGMLFGAIALMMGIIHFSFGPFSAPPPTFESIV
                     ADKTAEIKRGLLAGIKGEKITTVEKKEDVDVDKILDQSGIALAIAALLCAFIGGMRKE
                     NRWGIRGALVFGGGTLAFHTLLFGIGIVCSILLIFLIFSFLTGGSLV"
     gene            60428..60775
                     /gene="yidH"
                     /locus_tag="ACHKTW_000479"
     CDS             60428..60775
                     /gene="yidH"
                     /locus_tag="ACHKTW_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HFO6461561.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            60765..61127
                     /gene="yidG"
                     /locus_tag="ACHKTW_000480"
     CDS             60765..61127
                     /gene="yidG"
                     /locus_tag="ACHKTW_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HFO6461562.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            61124..61621
                     /gene="yidF"
                     /locus_tag="ACHKTW_000481"
     CDS             61124..61621
                     /gene="yidF"
                     /locus_tag="ACHKTW_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFO6461563.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRAVQLI
                     PYLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     QPRRDCQACDWHRLCPHARQETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(61629..62813)
                     /gene="emrD"
                     /locus_tag="ACHKTW_000482"
     CDS             complement(61629..62813)
                     /gene="emrD"
                     /locus_tag="ACHKTW_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HFO6461564.1"
                     /translation="MKRQRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSDQGQ
                     PV"
     gene            62895..62969
                     /gene="ysdE"
                     /locus_tag="ACHKTW_000483"
     CDS             62895..62969
                     /gene="ysdE"
                     /locus_tag="ACHKTW_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsdE"
                     /protein_id="HFO6461565.1"
                     /translation="MNVSQIYARNGELFSGRICKQKRQ"
     gene            complement(63232..63321)
                     /gene="tisB"
                     /locus_tag="ACHKTW_000484"
     CDS             complement(63232..63321)
                     /gene="tisB"
                     /locus_tag="ACHKTW_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HFO6461566.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            63886..63984
                     /gene="ivbL"
                     /locus_tag="ACHKTW_000485"
     CDS             63886..63984
                     /gene="ivbL"
                     /locus_tag="ACHKTW_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HFO6461567.1"
                     /translation="MTTSMFNAKLLPTALSAAVVVVRVVVVVGNAP"
     gene            64090..65778
                     /gene="ilvB"
                     /locus_tag="ACHKTW_000486"
     CDS             64090..65778
                     /gene="ilvB"
                     /locus_tag="ACHKTW_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HFO6461568.1"
                     /translation="MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPGPVWIDIPKDVQTAVFEIETQPAMAEKAAAPAFSEESIRDAAAMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPQVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYEQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            65782..66072
                     /gene="ilvN"
                     /locus_tag="ACHKTW_000487"
     CDS             65782..66072
                     /gene="ilvN"
                     /locus_tag="ACHKTW_000487"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFO6461569.1"
                     /translation="MQNTTHDNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIDKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            66146..66736
                     /gene="uhpA"
                     /locus_tag="ACHKTW_000488"
     CDS             66146..66736
                     /gene="uhpA"
                     /locus_tag="ACHKTW_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HFO6461570.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            66736..68238
                     /gene="uhpB"
                     /locus_tag="ACHKTW_000489"
     CDS             66736..68238
                     /gene="uhpB"
                     /locus_tag="ACHKTW_000489"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HFO6461571.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFPLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHPVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            68248..69567
                     /gene="uhpC"
                     /locus_tag="ACHKTW_000490"
     CDS             68248..69567
                     /gene="uhpC"
                     /locus_tag="ACHKTW_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HFO6461572.1"
                     /translation="MLPFLKAPADAPLMTDKYEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIINILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAASALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVISIAAGISALLLLPFLNAQAPREA"
     gene            69705..71096
                     /gene="uhpT"
                     /locus_tag="ACHKTW_000491"
     CDS             69705..71096
                     /gene="uhpT"
                     /locus_tag="ACHKTW_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HFO6461573.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(71141..>72869)
                     /gene="adeD"
                     /locus_tag="ACHKTW_000492"
     CDS             complement(71141..>72869)
                     /gene="adeD"
                     /locus_tag="ACHKTW_000492"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HFO6461574.1"
                     /translation="RAEYQELLAVSRGDAVADYIIDNVSILDLINGGEISGPIVIKGR
                     YIAGVGAEYADAPALQRIDARGATAVPGFIDAHLHIESSMMTPVTFETATLPRGLTTV
                     ICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCDVNGASFTLEQMLA
                     WRDHPQVTGLAEMMDYPGVISGQNALLDKLDAFRHLTLDGHCPGLGGKELNAYITAGI
                     ENCHESYQLEEGRRKLQLGMSLMIREGSAARNLNALAPLINEFNSPQCMLCTDDRNPW
                     EIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGLLAPGKQADIVLLS
                     DARKVTVQQVLVKGEPIDAQTLQAEESARLAQSAPPYGNTIARQPVSASDFALQFTPG
                     KRYRVIDVIHNELITHSHSSVYSENGFDRDDVSFIAVLERYGQRLAPACGLLGGFGLN
                     EGALAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNGQVQSHLPLPIAGL
                     MSTDTAQLLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPALKLTSQGLFDGEKF
                     AFTTLEVTE"
BASE COUNT        17650 a        18053 c        19200 g        17966 t
ORIGIN      
        1 aaatacactt attaatataa gtgatgattc atggtaaagg atatattata tcaatggagt
       61 ttttatgaag cgtaatatta taggcggtgc attcactctg gcatctctaa tgctggccgg
      121 gcatgcactg gcagaagatg gtgttgttca cttcgtcggt gaaattgtcg acactacttg
      181 tgaagttacc tccgatacag ccgatcaaat tgtcccactg ggtaaagtca gtaaaaatgc
      241 attttcaggt gtaggtagtc tggcgtcgcc acagaagttc agtattaagc ttgaaaattg
      301 cccggcaacg tacactcaag cagccgttcg ttttgatggt acagaagcgc ctggcggcga
      361 cggcgacctg aaagtgggta cgccgcttac agcaggcaac cctggtgatt ttaccggtac
      421 aggacaagcg attgcggcaa ccggcgttgg tattcgtatt tttaaccagt ccgataattc
      481 gcaggttaaa ctttataacg actctgctta taccgctatc gatgctgaag gcaaggctga
      541 aatgaagttt attgcacgct atgtggcaac caatgcgacc gtaacggctg gtacggcgaa
      601 cgctgattca caatttactg tcgaatataa gaaataattt atcaagggcc tttttattca
      661 gttggctttg gccagggagt cgttatgtcc agaaaaagtt gtttaatcag gctctttaaa
      721 acaacagttt catgtaagtt atttaccgcg attatactca gcgttttttt aggccaacca
      781 gcattaactt atgcaggcgt tgtcattggt ggtacgcggg tggtttactt aagtaataat
      841 gctgataaat ctatttctgt atttagcaaa gaagaaaaaa taccttatct catccaggct
      901 tgggtagatc catttaataa agaggataaa agcaaagcgc cgtttaccgt tattccgcca
      961 gtatcacgat tagaaccttc gcaagaaaaa atattacgca ttattcacac caaaggtgtg
     1021 tcattgcctg atgatcgcga gtcagtcttc tggctgaata ttaaaaatat tccgccatca
     1081 gcgagcaaca aagcgacgaa ctcgctggaa atagcagtaa aaacgcgtat caagctgttc
     1141 tggcgaccag caaatatccg ccttattccc gaagatgctg cgccgaaagt gaaatggcgc
     1201 cgggaagggc gaaatctgat agcagagaat cccaacccaa ttcatatcag tgtgatggat
     1261 gtcatcgttg atggccatga tgtgcccctc aatatgatcc gtccttttga aacgctcacg
     1321 ttacctttac ctgctaattc tgcgggaggc caaatgacct ggcgatttat taatgattac
     1381 ggcgcagtca gtgacccgat aaaaatgacg ctgtaagcaa gggataagga taggcgtatt
     1441 atgatgacga ccagaatagt ggttggcctc acggcaggga cgtgtctgat tttctcgcaa
     1501 aacctgatgg ccgaggtcag tgtattcaat ccggcgcttc tggaaatcga ccatcaatcc
     1561 ggagtcgata ttcgccagtt taatcgggca aacctgatgc ccccaggtgt ttatagcgtt
     1621 gatattttta tcaacggtaa aatgtttgaa cgtcaggatg tgacatttgt tcaggataat
     1681 ccagatgctg atctgcacgc atgctttatt gccattaaaa aaacactgtc ctcctttggc
     1741 ataaaagttg atgcgctcaa atcgttcaat gatgtggatg agacggtttg cctcgatcct
     1801 gctccacgta ttgaaggctc atcctggcag tttgacagtg ataaattgca gctgaatata
     1861 tccattcccc aaatctacat ggacgcgatg gcttatgatt acatcagccc cacgcgttgg
     1921 gatgagggga ttaacgcgct caccatcaac tacgattttt ctggttcaca tacactacgt
     1981 tcagattatg gttcacaaga gacagatacc agttatctca atctgcgcaa tggactgaat
     2041 attggaccgt ggcggctacg taattacagt actttaaaca ccagcgatgg ccgtgcggaa
     2101 tacaactcca ttagtacctg gatacagcgc gatattgccg cgttaagaag ccagattatg
     2161 attggtgata cgtggacggc gagcgatatt ttcgacagta cgcaaattcg cggcgcgcgt
     2221 ttgtatactg ataacgatat gctacccgcc agccagaatg gctttgctcc tgtggttcgt
     2281 gggattgcaa agtccaacgc caccgtcatc attcggcaga atggctacgt gatttatcag
     2341 tctgccgttc cacaaggtgc ttttgagatc accgatctca acaccgccag tacaggtggc
     2401 gatttggacg taaccatcaa agaagaagac ggtagcgaac aacgattcac ccaaccttat
     2461 gcttcattgg cgattcttaa acgtgaaggt cagacagatg ttgatgtcag cgtgggtgaa
     2521 ttgcgcgatg aagacggatt tacaccggac gtccttcagg cgcaaatact tcatggtttt
     2581 tcccacggga tcactttata tggaggtatg caggctgctg aaaattatgg ttctgcagct
     2641 ctgggtgtcg gtaaagatct tggcgctttg ggcgcaattt ctttcgatgt gacacatgct
     2701 cgtgcgaatt ttagccatga tgatacagaa acgggtcagt catatcgctt tctctattca
     2761 aaacgatttg acgacacaga cactagcttg cgcctggttg gctatcgtta ctccaccgag
     2821 ggctactata ccctcaatga gtgggcatcg cggcgcaaca gccctgaaga cttttgggaa
     2881 acaggtaacc gacgtagtcg cgtggaggga acgctaacgc agtcgttggg gagagattat
     2941 ggcaatttat acctgacatt aagccggcaa caatactggc ataccgatga tgtcgaacga
     3001 ttaatgcaat ttggctacag cagtagctgg aagcgtctct cgtggaacgt ctcctggagt
     3061 tattccaata ctgccagaca ggggacgggg aacaaccatg ccagtgataa caccagtgag
     3121 cagatctaca tgctctcttt atctgttcct ttatcgggct ggtggggtaa tagttacgcc
     3181 acctattctg tttcgcaaaa cgataattcc ggtagctcac atcaactcgg actcagcggt
     3241 acggcgctgg aaagaaataa cctttcatgg aatttaatgc agtcctataa cagtcatgat
     3301 gatgaggttg gcggtaatat gtccctgacc tatgatggct cttatggcac ggtgaacggc
     3361 agctataact acagccaaaa ttcccagagg ctgaattatg gtatcagagg gggaattctg
     3421 gcacacagcg aaggggtaac gttaagtcag gagttaggtg aaactattgc tcttgttaaa
     3481 gcacctgggg ccgccgggtt agaaatagat aatatgcgcg gtgctgcgac ggactggcgc
     3541 ggctatacgg tcaagacaca gctaaaccct tatgatgaaa atcgggtagc aatcagcgat
     3601 aactatttct cgaagtcgaa tatagaactt gataataccg tcgttacgat ggttcccacg
     3661 cgtggtgcag tggttaaagc ggagtttgtg actcatgtgg gttatcgcgt tctcttcaga
     3721 gtgttaaatg caaatggtaa accggtacct tttggagcca ttgctgcgat acaagatgca
     3781 agtttggcag attcaggaat tgtcggtgac cgtggcgaac tttatctttc tggtctacca
     3841 gaaaaaggac aggttacgtt atcctgggga gaaaacgcct caacaaaatg catcttcaat
     3901 tattcacttt cgacaccaga aagtgagagc ggattaattg aacagggtgt gacatgtcat
     3961 taaaaggatt tttatgaaca agtatatcaa acagtggtgc tttgctgtgt ttatgctctc
     4021 gttaagtagt gtagcacttg cagctcctaa aggtatctgt acccctgata atggcgtatt
     4081 tcacagtacg cttgatttct cgggatatct tattaccgcg aacgaaaata aggttggaac
     4141 gacatttaac accactgtaa ctaatggatc ctcatatcct ggacgctgtc attgtgatac
     4201 tggtaacgta ggagaattcc cctatatcta ttatacgtca aaaataaacc aggcattaac
     4261 ttatgctggg gttcactcta atattaatta ttatgatctt aatccgaacc ttgatgttgg
     4321 gattgctata gatattcttg gggttgggta tgtgaatgca ccttttgaat atcatgctaa
     4381 caacccgtct ggtaatacaa agtacaattg caaccgcatt gaacctttaa gtatatctag
     4441 tggggctaaa gcgatagtat atttttatat caagaagaca tttgcaggaa aattgattat
     4501 tcctgaaaca aagatagtga cattgtatgg aacaattagc cgtgacaccc cggtggatta
     4561 ctcacagccg atggctgatg tttatattcg gggcgatatt acagctccgc aaagttgcga
     4621 aataaataat ttacagccag tttattttga ttttaaagaa ataccagctg cagatttttc
     4681 atctgttgtt ggaagtgcgg taacaacaca taaaattact aaaacggtca ctattgagtg
     4741 tgaaaacctg gggatactga ataccgatga tatcagtacg tctttttatg ctaccgaacc
     4801 caatacggac aattcaatgg tcgtaacttc aaactctaac gtggggataa aaatttacga
     4861 caaaaataat aaggaaatca aagtgaacgg tggtgagttg ccaacagaca tgggtaaatc
     4921 aacagtgtat ggtgaaaaat caggtagtgt aactttttca gctgctcctg cgagccttac
     4981 tggagctcgc ccagccccgg ggcaatttac tgcaacagcg acgataactg ttgagattgt
     5041 gcgctaatag gtctgacaac gattgttcat cacatcgttg tcatctcacc catttcttta
     5101 tcacttttaa atgcatcatg acaaaaagat gacatgtggg attttcatct tcacatttaa
     5161 aatcagtaag ttatttggta attcctttct gtcataaaaa tgtcatattc cttacatata
     5221 actgtcacct gtttgtccta ttttgcttct cgtagccaac aaacaatgct ttatgaatcc
     5281 tcccaggaga cattatgaaa gttatgcgta ccaccgtcgc aactgttgtc gccgcgacct
     5341 tatcgatgag cgctttctct gtgtttgcag aagcaagcct gacaggtgca ggtgcaacct
     5401 tccctgcgcc ggtgtatgcc aaatgggctg acacttacca gaaagaaacc ggtaataaag
     5461 ttaactacca gggtatcggt tcttccggtg gcgtaaaaca gattatcgct aataccgttg
     5521 attttggtgc ctctgacgcg ccgctgtctg acgaaaaact ggctcaggaa ggtctgttcc
     5581 agttcccgac cgtgattggc ggcgtggtgc tggcggttaa cattccaggg ctgaagtctg
     5641 gcgaactggt gctggatggt aaaaccctcg gcgacatcta cctgggcaaa atcaagaagt
     5701 gggatgatga agccatcgcc aaactgaatc cgggtctgaa actgccttca caaaacattg
     5761 ctgtagtacg ccgcgcagat ggctccggga cttccttcgt cttcaccagc tacctggcga
     5821 aagtgaacga agagtggaaa aacaacgttg gtactggctc taccgtaaaa tggccgatcg
     5881 gtctgggcgg taaaggtaac gacggtatcg ccgcgttcgt tcagcgtctg ccgggtgcaa
     5941 ttggttatgt tgaatatgct tacgcgaagc agaacaacct ggcgtacacc aaactgatct
     6001 ccgctgatgg taaaccggtt agtccgaccg aagaaaactt cgctaatgca gcaaaaggtg
     6061 cagactggag caaaaccttc gctcaggatc tgaccaacca gaaaggcgaa gatgcatggc
     6121 ctattacctc taccacgttc attctgatcc acaaagatca gaagaaacca gaacaaggca
     6181 cagaagtgct gaagttcttc gactgggctt acaaaaccgg ggctaaacag gcgaacgacc
     6241 tggattacgc cagcctgccg gatagcgtag ttgaacaggt tcgcgctgcg tggaagacca
     6301 atattaaaga cagtagcggt aagccgctgt actaataaaa ctccaggccg ggtacggtgt
     6361 tttacgccgc atccggcatt acaaaatgac tttgtaaacg cgtttaactg aagagtaact
     6421 tatggctgca accaagcctg cttttaaccc accgggtaaa aagggcgaca taattttcag
     6481 cgtgctggta aaactggcgg cgctgattgt gctattgatg ttgggtggca ttattgtctc
     6541 tctgatcatc tcctcctggc cgagcattca gaaatttggt ctggctttcc tatggaccaa
     6601 agagtgggat gcaccgaacg atatctacgg ggcgctggtg ccgatctacg gtacgttggt
     6661 gacttcgttt atcgcgctgc tgatcgccgt cccggtgagt ttcggtatcg ccctgttcct
     6721 gactgagctt gcgcctggct ggctgaaacg cccgctgggt atcgccattg agctgctggc
     6781 agccattcca agtatcgttt acggcatgtg gggcctgttt atctttgcgc cgctgttcgc
     6841 cgtttacttt caggagccgg tcggcaatat catgtcgaat atcccgattg ttggcgcgct
     6901 gttctctggc cccgcatttg gtatcggtat cctcgcggca ggcgtgatcc tcgccatcat
     6961 gattattccg tacattgcgg cggtaatgcg tgatgtgttc gaacaaaccc cggtgatgat
     7021 gaaagagtcg gcctacggta ttggctgcac cacctgggaa gttatctggc gtatcgttct
     7081 tccgttcacc aaaaatggtg ttatcggcgg catcatgctg gggctgggcc gcgcgctcgg
     7141 tgaaaccatg gcggtgacct ttatcatcgg taacacctac cagctcgaca gcgcctcgct
     7201 gtatatgccg ggcaacagta tcacctctgc gctggcgaac gaatttgcgg aagcggaatc
     7261 cggtctgcac gttgccgcac tgatggaact gggcctgatc ctgtttgtga ttaccttcat
     7321 cgtcctcgcc gcatcgaagt ttatgattat gcgcctggct aagaatgagg gggcacgcta
     7381 atggctatgg ttgaaatgca aaccactgcg gcgctggctg aatctcgccg caaaatgcag
     7441 gcgcgtcgcc gcctcaaaaa ccgtattgcg ctgacgctct cgatggcgac gatggccttc
     7501 ggcctgttct ggctgatctg gattttaatg tccaccatca ctcgcggtat cgacggtatg
     7561 tcgctggcgc tgttcactga aatgacgccg ccgcccaata cggaaggtgg tggtctggcg
     7621 aacgccctgg cgggtagcgg gctgttaatt ttgtgggcca cggtattcgg tacgccgctg
     7681 ggcattatgg cggggattta tctggcggaa tatggtcgta aatcctggct ggcagaagtg
     7741 attcgcttca ttaacgacat tctgctctcc gcgccgtcga ttgtggttgg tctgtttgtt
     7801 tacaccatcg tggtggcgca gatggagcac ttctctggct gggcgggtgt aattgcgctg
     7861 gcgctgttgc aggtgccgat tgttatccgc accaccgaga acatgctgaa actggtgccg
     7921 gacagcctgc gtgaagcggc ttatgcgctg ggtacaccga agtggaaaat gatctccgcg
     7981 attacgctga aagcgtcggt gtccgggatc atgaccggta tcctgctggc gattgcccgt
     8041 attgccggtg aaaccgcgcc gctgctgttt accgcgctct ccaaccagtt ctggagcacg
     8101 gacatgatgc agccgatcgc caacctgccg gtgacgatct ttaagtttgc gatgagcccg
     8161 tttgcagaat ggcagcaact ggcctgggcc ggggtgttga ttattaccct gtgcgtactg
     8221 ctgctgaaca ttctggcgcg cgttgttttt gcgaagaata aacacggtta acactttgcc
     8281 ggatgcggcg tgaacgcctt atccggccta cggtaagcct gattcgcgaa gtgcatcagg
     8341 cacgatgagg aaaagattgc aatgagtatg gttgaaactg ccccgagtaa aattcaggtt
     8401 cgtaatttga acttctacta cggcaaattc catgccctga aaaacatcaa cctggatatc
     8461 gctaaaaacc aggtaacggc gtttatcggg ccgtccggct gcggtaaatc gacgctgctg
     8521 cgtaccttca acaaaatgtt tgaactgtac ccggagcagc gtgcggaagg tgaaattctg
     8581 cttgatggcg acaacatcct gaccaactct caggatatcg ccctgctgcg tgcgaaagtg
     8641 ggcatggtgt tccagaaacc gacgccgttt ccgatgtcca tctacgacaa catcgctttt
     8701 ggcgttcgtc tgtttgagaa gctctcccgt gccgacatgg acgagcgcgt gcagtgggca
     8761 ttgaccaaag ccgcattgtg gaacgaaacc aaagataaat tgcaccagag cggttactct
     8821 ctctctggtg gtcagcaaca gcgtctgtgt attgcgcgtg gtatcgccat tcgcccggaa
     8881 gtgctgctgc tcgacgaacc gtgttcggcg ctcgacccta tctctaccgg gcgtattgaa
     8941 gagctgatca ccgaactgaa gcaggattac accgtggtga tcgtcaccca caacatgcag
     9001 caggctgcgc gttgttccga ccacacggcg tttatgtacc tgggcgaatt gattgagttc
     9061 agcaacacgg acgatctgtt caccaagcca gcgaagaaac aaacagaaga ctacatcacc
     9121 ggtcgttacg gttgattcag gagtgcgtta tggacagtct caatcttaat aaacatattt
     9181 ccggccagtt caacgccgaa ctggaaagta tccgcacgca ggtgatgacc atgggcggca
     9241 tggtggagca gcagctttct gatgcaatca ccgcgatgca taaccaggac agcgatctgg
     9301 cgaagcgcgt gatcgaaggc gacaagaacg tcaacatgat ggaagtggcg atcgatgaag
     9361 cctgcgtgcg cattatcgcc aaacgtcagc cgacggcgag cgacctgcga ctggttatgg
     9421 tgatcagtaa aaccattgcc gagctggagc gtattggcga cgtggcggac aaaatctgcc
     9481 gtactgcgct ggagaaattc tcccagcagc atcagccgtt gctggtaagt ctggagtcgc
     9541 tgggccgtca taccatccag atgctgcacg acgtgctgga cgcgttcgcg cggatggaca
     9601 ttgacgaagc ggtacgtatt tatcgtgaag ataaaaaagt cgatcaggaa tacgaaggta
     9661 ttgttcgtca actgatgacc tacatgatgg aagattcgcg taccattccg agcgtactta
     9721 ctgcgctgtt ctgcgcgcgt tctatcgaac gtattggcga ccgctgccag aatatttgtg
     9781 agtttatctt ctactacgtg aaggggcagg atttccgtca tgtaggcggt gatgagctgg
     9841 ataaactgct ggcgggaaaa gatagcgaca aataattcac cagacaaatc ccaataactt
     9901 aattattggg atttgttata tataacttta taaattccta aaattacaca aagttaataa
     9961 ctgcgagcat ggtcatattt ttatcaatag cgcattgcta ttttctctgc acgcaattaa
    10021 attaatttcc gaacctggat gttcgttata aaaaccatta ataaatgact ggattgttac
    10081 tgcattcgca ggcaaaacct gacataacca gagaatactg gtgaagtcgg gtttttttgt
    10141 ttataaaaaa ggtccttgct atgaacatgc aaatcaccaa aattctcaac aataatgttg
    10201 tggtggttat tgatgatcaa cagcgggaaa aagtcgtcat ggggcgagga attggctttc
    10261 aaaaacgcgc tggcgaaaga attaactcaa gtggaataga aaaagagtat gccttgagca
    10321 gtcatgaact gaacgggcga ttaagcgaac tcttaagtca tattcctctt gaggtgatgg
    10381 caacctgtga tcgtattatc tctttagcgc aggagcgctt gggaaaatta caggacagta
    10441 tttatatctc gctaactgac cattgccagt ttgcgattaa acgctttcag caaaacgtgt
    10501 tgctgcccaa cccgttgctg tgggatatcc agcggcttta cccgaaagag ttccagctag
    10561 gggaagaagc gttaaccatt attgataaac ggttgggcgt gcagttaccg aaagatgaag
    10621 tgggctttat tgccatgcat ctggtcagtg cccaaatgag cggaaatatg gaggatgttg
    10681 caggtgtcac gcagttaatg cgcgaaatgc tgcaattaat aaaatttcag ttcagcctta
    10741 attaccagga agaaagcttg agttatcagc gactggttac acatctgaag tttttatcct
    10801 ggcgtattct tgaacatgct tcaattaacg atagtgatga atcattacaa caagcagtaa
    10861 aacaaaatta cccgcaagca tggcaatgtg cggaacggat cgccattttt attggtttgc
    10921 agtatcaacg taaaatttca cccgcagaga ttatgttttt agccataaat atagagcgcg
    10981 tgcgcaaaga acactgaaat attattactg agtaaaggat tgttaccgca ctaagcgggc
    11041 aaaacctgaa aaaaattgct tgattcacgt caggccgttt ttttcaggtt ttttttggag
    11101 ttttgccgca aagcggtaga gggcaagtta tgacggagtt agccagaaaa atagtcgcag
    11161 gagtcggggg cgcagataac attgttagtc tgatgcattg cgcaacgcga ttacgtttta
    11221 agttaaagga tgaaagcaaa gcgcaagctg aggtactgaa aaacaccccc ggtattatta
    11281 tggtggtgga aagcggtggc cagtttcagg tggtcatagg taaccatgtg gccgatgtct
    11341 tcctggcggt caacagcgtg gcaggccttg gcgaaaaagc gcaacaggca ccggaaaatg
    11401 atgagaaagg taatctgcta aaccgctttg tttatgttat ttcaggtatt tttacgcctc
    11461 tgatcggttt gatggcggca accgggatcc tgaaaggtat gctggctctg gcgctcactt
    11521 ttcagtggac gaccgaacaa agtggtactt atttaatttt attcagcgcc agtgatgcct
    11581 tgttttggtt cttcccgata atcctgggat acaccgcggg gaaacgcttc ggcggtaatc
    11641 catttactgc catggtgatt ggtggagcgt tagtgcatcc attaattctg actgctttcg
    11701 agaacgggca aaaagcggat gagctggggc tggatttcct gggtattccg gtcacattgt
    11761 tgaattactc gtcatcggtt attcccatta ttttttctgc ctggttgtgc agcattctgg
    11821 aacgccgact taatgcgtgg ttaccgtcgg caatcaaaaa tttcttcaca ccattgctat
    11881 gtctgatggt tatcacaccc gtcacctttc tgctggtggg gccgctatca acctggataa
    11941 gcgaactgat tgccgccggt tatctctggc tttatcaggc ggttcctgca tttgcgggcg
    12001 cggtaatggg cggcttctgg caaatcttcg tcatgttcgg actgcactgg ggcctggtgc
    12061 cgctgtgtat caataacttc accgtgctgg gctacgacac catgatcccg ctgttaatgc
    12121 ccgccattat ggcgcaggtc ggggcggcgc tcggcgtctt cctctgcgaa cgcgatgcgc
    12181 agaaaaaagt ggtggcggga tcagcggcgt tgacgggcct gtttggtatc accgaaccag
    12241 cggtatatgg cgttaatctg ccgcgtaagt acccctttgt tatcgcctgt atcagtgggg
    12301 ctttgggggc caccatcatt ggctacgcgc aaacgaaagt gtactccttt ggtttgccaa
    12361 gtattttcac cttcatgcaa accatcccgt caacgggaat tgatttcacc gtctgggcca
    12421 gcgttattgg cggtgtcatt gccatcggtt gcgcatttgt cggtacggtg atgcttcatt
    12481 tcatcaccgc taaacgtcag ccagcgcagg gtgccccgca agagaaaaca ccagaggtta
    12541 ttacaccacc tgagcagggc ggtatctgtt caccgatgac gggagagatt gtgccgctca
    12601 ttcacgtcgc tgataccacg tttgccagtg gcctgttggg taaaggtatc gccattctgc
    12661 cctcggttgg tgaagtgcgt tctccggttg cgggtcgaat tgcttcgttg ttcgccacat
    12721 tacacgctat tggcattgag tcagatgatg gtgtggagat cctgattcat gtcggtatcg
    12781 acaccgtaaa actggacggc aaattctttt ccgctcacgt caacgtgggt gacaaggtca
    12841 atacaggcga tcggctgatt tcttttgata tccctgctat tcgcgaggcc ggatttgatc
    12901 tgacgacgcc ggtattaatc agtaatagcg atgattttac agacgtatta ccccacggca
    12961 cggcgcagat aagcgctggt gagccgctgt tatccatcat tcgctaacga taaaaggagt
    13021 taattatgaa agcatttcca gaaacatttc tttggggtgg cgcaacagct gccaatcagg
    13081 tggaaggtgc ctggctggaa gatggcaaag ggatcacgac ctcagattta cagcctcatg
    13141 gcgtaatggg aaaaatggaa ccgcgcatcc tggggaaaga gaatatcaaa gatgtcgcca
    13201 tcgattttta tcaccgttac ccggaagata tcgcgttatt tgccgagatg ggcttcacct
    13261 gtctgcgtat ttccattgcc tgggcgcgaa ttttccctca gggcgacgaa gccgaaccga
    13321 atgaagcggg gttagcgttt tacgatcggc tgtttgatga aatggcgcag gcggggatca
    13381 agccgctggt aacgttatcc cattacgaaa tgccatatgg gctggtgaaa aactacggcg
    13441 gttgggctaa tcgggcggtc atcgatcact tcgagcatta cgcccgcacg gtctttaccc
    13501 gctaccaaca taaagtagcg ttatggctga cgtttaatga aatcaatatg tcgttacacg
    13561 cgccattcac gggcgtgggg ctggcagaag agagtggcga ggcggaagtt tatcaggcga
    13621 tccatcatca actggttgcc agtgcgcggg cagttaaagc ctgtcatagc ctgatccccg
    13681 aagcgaaaat cggcaatatg cttctcggcg ggctggttta ccccctcacc tgccagccac
    13741 aggatatgtt ggaggccatg gaagagaacc ggcgctggat gttctttggt gatgttcagg
    13801 cgcgtggtca gtatcccggc tatatgcagc gtttcttccg cgaccacaat atcaccattg
    13861 agatgactga aagtgacgca gaagatttaa aacataccgt agatttcatc tcttttagtt
    13921 attacatgac tggttgtgtt tcccacgacg aaagcattaa taaaaatgcg cagggcaaca
    13981 tactgaatat gatccccaat ccgcatctga aaagttcaga gtgggggtgg caaattgatc
    14041 cggttggatt acgggttctg ttaaatacgc tttgggatcg ttatcaaaaa ccgttattta
    14101 ttgtcgagaa cggattaggc gcaaaagaca gcgttgaagc ggatggttcg atacaggacg
    14161 attatcgaat tgcctattta aacgatcacc tggtacaggt aaatgaagcg attgccgatg
    14221 gtgtggatat tatggggtac accagttggg ggccaattga tttagtcagt gcatctcatt
    14281 cacaaatgtc taagcgctac ggctttattt atgtggatcg tgatgataat ggcgaaggaa
    14341 gcctcacaag aacgcgtaag aaaagcttcg gatggtatgc agaagtgatc aaaacgcggg
    14401 ggctgtcatt aaaaaaataa ccaataaagc accttaatta tcgtcgcatt cagaacagtc
    14461 tggatgcgat acgttaattc tttctttgca ccataaaggg atattatgtt tagacgaaat
    14521 cttattacct ctgccatctt attaatggca ccgttagcct tttctgcaca atcattggct
    14581 gaatcattaa cggtggaaca tcgccttgag ttattagaaa aagcgttaag agaaacgcaa
    14641 agcgaactca aaaagtataa agatgaagag aagaaaaaat atacgccagc gacggtgaat
    14701 cgtagcgtaa gtacgaatga tcaagggtat gccgccaatc cgttcccgac cagtagtgcc
    14761 gcaaaacctg atgctgtact ggtcaaaaat gaagagaaaa atgccagtga gacaggctcg
    14821 atttattctt ccatgactct gaaagatttc agtaagtttg tgaaagatga aattggcttt
    14881 agttacaacg gctattaccg ttctggttgg gggaccgcct ctcatggttc acctaaatca
    14941 tgggcgattg gttctctggg ccgctttggt aacgaatact ccggctggtt tgatttgcag
    15001 ttaaaacaac gtgtctacaa cgaaaacggc aaacgggttg atgccgttgt gatgatggat
    15061 ggtaacgttg gtcagcagta ctctaccggc tggtttggcg ataacgccgg tggcgagaac
    15121 tttatgcagt tctccgatat gtacgttacc accaaaggtt tcctgccctt tgcgccagag
    15181 gctgatttct gggtgggtaa acacggtgcg ccgaaaattg aaatccagat gctggactgg
    15241 aaaacgcagc gtactgatgc cgcagcgggt gtaggtctgg aaaactggaa agtcggtccg
    15301 ggtaaaattg atatcgcgct ggttcgcgaa gatgtcgatg attacgatcg cagcctgcaa
    15361 aacaaacagc agattaatac caatactatt gatttacgct ataaagatat cccgttatgg
    15421 gataaagcga ccttaatggt gagtggtcgt tatgtcacgg caaacgaaag cgcatcggaa
    15481 aaagataatc aggataataa cggctattat gactggaaag atacctggat gtttggcaca
    15541 tctttaacgc agaaatttga taaaggtggc ttcaacgaat tctccttcct ggttgcgaat
    15601 aactctatcg ccagtaactt tggccgttat gctggcgcaa gtccatttac cacctttaat
    15661 ggtcgttatt atggtgatca caccggcgga acagcagttc gtctgacttc acagggcgaa
    15721 gcctatatcg gcaatcattt cattgtggct aacgcgattg tttactcctt cggtaacgat
    15781 atttatagct acgaaacagg cgcacactct gatttcgaat ctattcgtgc ggttgttcgc
    15841 ccggcctata tttgggacca atataaccag acaggtgttg aactgggcta tttcacccag
    15901 caaaacaaag atgcgaatag taataaattt aatgagtctg gttataaaac cacgctcttc
    15961 cataccttta aagtcaatac cagtatgttg acctcgcgtc cggaaattcg tttctacgcc
    16021 acgtatatca aagccctgga aaacgaactg gatggcttca ccttcgaaga caataaagac
    16081 gaccagtttg ctgtcggtgc ccaggctgaa atctggtggt aatggaactt aatgaaagga
    16141 atattacgat gaatataaaa attgctgctt taacgctggc tattgccagc ggtatttctg
    16201 ctcagtgggc catagcagcg gatatgccag ccagcccggc acccactatc ccggtgaaac
    16261 agtatgtgac tcaggtcaat gccgataaca gcgtgacctt tcgctacttt gcccctgggg
    16321 caaaaaatgt ctctgtagtg gtgggtgttc cggttctgga caatattcac ccgatgacca
    16381 aagacgaagc aggagtctgg tcgtggcgca cacctatcct gaaaggcaac ctgtacgagt
    16441 attttttcaa tgttgatggt gtacgcagca ttgatacagg caccgcaatg actaagcctc
    16501 agcgccaggt taactccagt atgattctgg tgccaggcag ttatctggat acgcgttctg
    16561 ttgcgcatgg tgatttgatc gccataactt accactccaa cgcattgcaa tctgaacgtc
    16621 agatgtatgt ctggaccccg ccaggataca ccggcatggg cgagcctttg ccagtgctct
    16681 atttctatca cggttttggt gataccggac gttccgctat cgatcagggg cgtatcccgc
    16741 aaatcatgga taacctgctg gctgaaggga aaattaaacc gatgctggtg gtgatcccgg
    16801 ataccgaaac cgatgcgaag ggcattattc ccgaagattt cgtgcctcag gaaagacgta
    16861 aagtctttta tccgctgaat gctaaagcgg cagatcgcga actgatgaac gatattatcc
    16921 cgctgattag caagcgtttt aacgtcagta aagatgccga tggtcgcgca ctggcagggc
    16981 tttcacaagg tgggtatcag gcgctggtat cgggaatgaa tcatctggaa agctttggct
    17041 ggctggccac attcagtggt gttaccacga caaccgtacc ggatgaaggt gtcgcggccc
    17101 ggctgaacga tccggcagct attaaccagc aactacgtaa ttttactgtg gtcgttggtg
    17161 ataaagatgt cgtaaccggc aaggatatcg ccgggctgaa aactgagctt gagcagaaaa
    17221 aaattaagtt tgattaccag gaatatcccg gcctgaacca tgaaatggat gtctggcggc
    17281 ctgcctatgc agccttcgta cagaaattat ttaaataaaa taaggcacta agatgaaatt
    17341 aatcattacc gaagattacc aggaaatgag ccgtgtcgcg gcacaccatc tgcttggtta
    17401 tatgtcgaag acgcgtcgtg ttaacctggc aattaccgcc ggtagcacgc ccaaaggcat
    17461 gtatgaatac ctcaccaccc tggttaaagg taagccctgg tacgataact gctatttcta
    17521 taatttcgat gaaattccct ttcgcggcaa agagggagaa ggtgtaacga ttaccaatct
    17581 gcgtaatctg tttttcaccc ctgcggggat caaagaagag aatatccaga agctcactat
    17641 tgataactac cgcgagcatg atcagaaact ggcgcgtgaa ggcggcctgg atttggtggt
    17701 gctgggatta ggtgcagatg gtcatttttg tggcaatttg ccgaatacga cccatttcca
    17761 tgagcaaacc gtggagttcc cgatccaggg agagatggtt gatattgtgg cgcatggtga
    17821 gttgggcggc gacttttcgt tagtgcctga cagctacgtg acgatggggc cgaaaagcat
    17881 catggcggcg aaaaacttgc tgataattgt cagtggcgca ggtaaagcgc aggcactgaa
    17941 aaatgttctg caaggtccgg ttaccgaaga tgttccagct tccgtgttgc agctgcatcc
    18001 ttcgctgatg gtaatcgctg ataaagccgc tgccgcagaa ttagcgctcg gttaagctga
    18061 agaaataagg ccggatgtat gaattacgct ccggcctgtt ctcattattt aaataactac
    18121 gaaaaatcac cccacagtct cagcttgccg cagtggagac agcggaagag ataaccctga
    18181 caatcaccac ccttttgcag gcatttagct agatcagaat ttctaatacc gaaattctca
    18241 caatcttctt caaggttggc aaattcatcg aggcgatctt ttatatcatt ccagcccaca
    18301 tagccgataa aagcacagaa atcgccacaa tgcgcaagcc agaattcctg ttgccatcca
    18361 tgataacctg gcgtgcgttc aaccaactct ttcagcattt catcaggata tgtgttctta
    18421 ataccggcaa attcgtcctc ttcatcatac tcaaattcaa caccttctat gctggcatca
    18481 tcctgaaaac tacctgcaaa cttttcagca gcagaaccgt ccgcaataca ccacggacag
    18541 agatgttcaa cttcatcaac gcaataaaag ggacccgagt aataaactga cgtctgttgt
    18601 tcacagcaat cgcactctac ggttttatcc tgttcaaatg cgcctgtttc cagtggcttg
    18661 ggatgatatt tgaattgggg taacggcctg atattttgag tcatagtaac atcctgtctg
    18721 cttttgagta gcccaaagat aaagtatccc gcttaactta aagagtctgt ttcaggacat
    18781 gaaatatcca atccgggcag acgggtaatg attctgctca tcaccacacc tgccagcaat
    18841 gcaggaatga tgcggacaac gagactgtca ccagaaagaa tgttttcaaa caactccgag
    18901 gaagccatgc ccttaatatg tacaataact gcacaataga tctcggtgat aacgacgcca
    18961 cctatgccac cagccagaca acgataattt ttctgtgacc cgatcttttc cggcagacag
    19021 gcgaccatca cgccggttaa caatgcagga ataactcccg tagtaatcga caataagaga
    19081 aacaatggaa gtacctgtat tcttcgcaaa agaaccagcg gttcttttgc gaagaatatg
    19141 tagaggacaa gaaacagaac accaattaac ggtcctaaaa ccgcaaaata aagtccgtga
    19201 tgtattaccc gacgtgaaac agacatatca aactcccttt acttaccttt gagcgccaat
    19261 ttaagcgggg taagtgaagg gagtgtgaag aactatgccg taatatccca accacgcgct
    19321 ttccacagtt ccggtaactg cgaaagatgg gtaaaggtgg tgactttcgg gtgaacgatc
    19381 ggcttattgt gcgggtcggc gcagaagtag aacacttcca tacctgcgtc gataccagat
    19441 tgcgcaccgg caactgagtc atcaaccaga atgcagtttt ctacatttac attcatcgct
    19501 tttgccgcat ggaacattaa cgccgggtct ggcttccagc gctgaatatc gtagccgctg
    19561 aacagtttat ccgggaagta gtgcaacata ttcagcttgc ccatagaatg ctgcattttg
    19621 ttatttgggc cgttagatac cacacacatt ggcgcagtga tcgctgacag gagcgctcca
    19681 gccccttcga tggcttccag ttctgaatcg aacagccgag cgacttctgc acggtaaacg
    19741 tgttcagctt ctgttttcgc taacgtaaca ccatgttcaa gggaaacaat atcgataatt
    19801 tcgtacagtt ttacaccttt gaaacgtttg aatacctctt caggatcgag cgtaatacca
    19861 aattcctgaa acatcgttac atatgcgcgg gagcaaatga cttcactgtc gaccagcgta
    19921 ccgtcgcagt cgaaaaatac cgcttctatc tgggacatgt ctttccttta tcgttagttt
    19981 aacgttttcg tcatgccaat tcgcatgacg caatcgttgc cgtataagca aatacaatga
    20041 aaaaaagtta tcttcagccg tccatattgt ctcattttgg tatacgatag cgacggattt
    20101 tccctccttg ttcggaaata gataatgagt caacaacaca caacccaggc ttctggccag
    20161 gggatgctgg aacgcgtgtt taaactgcgc gaacatggca cgacggcacg gaccgaagtg
    20221 atcgccggtt ttaccacctt cctgacgatg gtttacatcg tttttgttaa cccgcaaatt
    20281 cttggcgttg ctggcatgga taccagcgcc gtcttcgtca ctacctgtct gatcgctgca
    20341 ttcggcagta ttatgatggg actgtttgct aacctgccag ttgcactggc acccgctatg
    20401 ggcctgaatg cgttcttcgc ttttgtcgtt gtacaggcga tgggcttgcc gtggcaagtc
    20461 gggatgggcg caatcttctg gggtgcgatt ggtctgctgt tactgacgat tttccgcgtt
    20521 cgctactgga tgattgccaa cattccggta agtctgcgtg tgggcatcac cagcggtatc
    20581 ggtctgttca ttggtatgat ggggctgaag aatgcaggtg tgattgtcgc taacccggaa
    20641 acgctggtca gcatcggtaa cctgacttct cacagcgtgc tgctggggat cctcggcttc
    20701 ttcatcattg ctattctggc ctcgcgcaac attcacgcag cggtgctggt ttctatcgtg
    20761 gtgacgacgc tgctgggctg gatgctgggt gatgtgcact acaatggcat cgtttctgcg
    20821 ccgccgagcg taatgacagt tgtgggtcat gtagatttag ccgggtcgtt taacctcggg
    20881 ctggcagggg tgattttctc tttcatgctg gtcaacctgt ttgactcctc cggtacgctg
    20941 attggcgtga ccgataaagc aggcctggcg gatgagaagg gtaaattccc gcgcatgaag
    21001 caggcgctgt atgtcgacag tatctcttcc gtgaccggtt cgtttatcgg tacttcttcc
    21061 gttacggctt atattgagtc ctcttccggc gtatcggttg gcggtcgtac cggtctgacg
    21121 gcagtggttg ttggtctgct gttcctgctg gttatctttc tgtcgccgct ggcggggatg
    21181 gtgccaggct acgctgcagc tggtgcgctg atttacgttg gcgtgctgat gacttccagt
    21241 ctggcacgcg tgaactggca ggatcttact gaatctgttc cggcgtttat taccgccgtg
    21301 atgatgccgt tcagcttctc gattaccgaa ggtatcgcgc tgggctttat ctcctactgc
    21361 gtgatgaaaa tcggtaccgg acgtctgcgt gatctaagcc cgtgcgtaat catcgttgcg
    21421 ctgctgttta tcctgaagat tgtctttatc gacgctcact aaaatcgata gcgcgttaaa
    21481 aacccgccat gcatgattgc tggcgggttt tttatttaga tcttaactcg ctgaataaac
    21541 tcaccaaatg cggtcaattg cccggtcagg tggtccagcg taccctgatc aatcacttct
    21601 ccggtttgcg gatcaacttt gttctgaatc acgccgccca taaattccgg cttgttcatc
    21661 accattgcat cgaggaaaac cagaatctgg cgcaggtgat actgacagcg cgcgccgcca
    21721 atcacgccca ttgagctggt ctgaatcaac accggtttac cggccagcgg ttgatccggc
    21781 aggcgggaaa gccagtcgat ggcatttttc agcccacccg gtaccgagta gttatattcc
    21841 ggcgtgacga tcaccacacc gtcagcctga cggatctgtt ccgccagagc ttcaaccgtt
    21901 gctggaaaac cttcttcctg ctgtacgtca gcgtcataca agggaatgtc ggcaatggaa
    21961 ggtaacgcat tgacttccat gctcgccgga gcaattttcg gcagggtacg tgcaaccatg
    22021 ccattaaatg agcctttgcg caggctcccc agtaacgtaa ccacctgcaa tttttcagac
    22081 atgatgactc ctgtggttgt ctatcaattt ggcgaaaaag ccttcacggt agagagctgg
    22141 gcaaaacgca tcatccggct ggtaggattg ttggcgcggc tgtgaatatc cggcaggctt
    22201 ttccagccgt gtttgccatc gaactcccag acactgagct tttcaatcgt gggcgtgacc
    22261 gccacggacc acaccagcac gtcctccgcg tcgcataacg cttctacatg atttacatga
    22321 gcacatttca ggcgtccggc aatgggcagc agctgcagat cgcgcggatc gtcattcaaa
    22381 acatcaccgg ttagttttag cacgcttcgg cgcagcgtga tgagctgcgc ccgcgcttcg
    22441 ttaggatcgt tttgtttact gatccatagc gattcattgc tggaaaaggt gtggttatcg
    22501 ggcgcgtgtg ggctatgaaa cccgcgcgtc gcacgctgta gttccatatc gagaccacat
    22561 tcaccttcgg ttgttaacgc cacgccaacc atattcccgg cataggaaat agaaaacgaa
    22621 ggcaaatttt tatcactgaa aaccggttta cctttcggca gggtgacgat ttccggcaat
    22681 tcgccaatgc catacagcat gaacatcagt tctgcgagta aacctctaga cgccagaaaa
    22741 cggctttgac gatgaggggg aatgtttcgg gcttcttgat gacactggga ggggagacga
    22801 acagaaatca gatgtccttc cgttaaaatc cctcgggcaa agtgagtcgc catttttcgc
    22861 tccatgctta atggtccatt atgaatacaa gagtaatcat ttactggaat gatggacaga
    22921 ctaaagcggc taaaagttcg attcagacaa gcttattaca acttttttac attttattgt
    22981 gaaattgggt cgcgtttcat acgattcggt tatgccatcg acgcgtaaag gtttttaatg
    23041 gtttcccgta accagacgat cttcggatta tggctgttcc gtttatgcca cagcagggta
    23101 aaaggaactt ccagcttttt ttgctggctt tcgtcaaacg ggagaggaag agcaaccaac
    23161 ggcagttgat ggagttgatt gtagtactga cagtagcgcg gcgcggtcgc cagtagcaga
    23221 ttgtcgggtt gcgctgccat aaacagtgac tgctcaaatt ccggcaggct catagcaatc
    23281 gtgcgttcgc gccccagctc ctgtaacaca ttgtccagcg cccaggtgtc gctctgttcc
    23341 cagcaaatgc tgatatgcgg ataacgtaaa aaggtgtcca gattccacgt ttcatgcagt
    23401 gccggatgat ctttgcgtaa ccagacgcag ggtacatcac taaacagcac ttcataatca
    23461 atggctaacg gtagcgagct taacagctcc cgcgagcgag gatggctttc gcgaccggaa
    23521 aaaccaatat ccacttcacc acgagtaatg gcatctaagg aatcgtaatc ccagttacgt
    23581 agtttgatgg tcgcctgcgg gtaacgttgg tagatccgtt tcgacagcgc attaagcatg
    23641 atcatcatca gcggcgattc cgccgccaac tcaaacttca ggccgcgcgg tgtctggtgg
    23701 tgcggtttat ccagcagcag attgctcatt tgcatccatt ccgccagatt ttgctccatg
    23761 ctgaccatta gcggtgtggg cgacaggccc agcggtgagt tcacaaagag cgggtcgtca
    23821 aaccacgctc ttaactttgc cagcgactta ctcaccgctg aaggggtcac gttcatccgc
    23881 ttcgccgctt tggtcacgct gcgctcctgc atcagcagtt gcagacagag cagcagattg
    23941 agatcaagcg tggtgatgga tttcttcatg agcggcaacg gggcgtccag gcgcgacgaa
    24001 cataatcagc aacaggctca ctatgctaca ggcaatcaga atcccgatca gcatattcca
    24061 tgcgccgata ccaaccaccg ctgccagcca aatccacagt gacgaaccgc aaacctgcgc
    24121 aatacctaag gtcgagctgg ctacgcccgc gcgtaatgaa aatggtccta acgcctgact
    24181 catcgctacg ccaaaaccta ccgagaaacc ggcgcaaatc agcgtgatac caaacagaga
    24241 aaccgcatgg gaaggtgaaa cggcaagggt gatccccgct gcaaggaata acacctgcga
    24301 ggtgatcatc aacgtacgtg gcttaaaaat tcccagcgca aatggcgtgg agaatgaaac
    24361 ggtcatgctg acgccagcag tcaacgccat aatggtggcg tattctccgc gctcaaaacc
    24421 catgatttcc atcagcaata ccggcgatgt attgacgaaa gtgaggatca ccgaaacgct
    24481 gagggtggtg ataacaacac ggctgaggaa aaaccgatta agcagcgact cgctattttc
    24541 tcgtggtttg tccgaagcag cgggggacgc tgggcgcgtt tcttttaaaa taaacaaaga
    24601 caacatcagt accgcgatgc ccatcattgc catcgcccag aacagactct gccacgggaa
    24661 tttaagcata atcagatgtc cgagcactgg cgctaacacc ggaatgatgc aggtaatacc
    24721 gttgagtaat gacagcactt tagcccgacg tcgatcatcc agcgtgtcgc gcaaaatggc
    24781 gaacgccact acgtaacaac agcctgcgcc caacccctgt agaaatcggc ctgcaagaaa
    24841 taacgtgctg gtttcagcca gtgaacagaa caccgaagcg ataataaata gcgccgcgcc
    24901 ggggatggcg acaggctttc tccctgaacg atcggccact taccggcaaa taacatcgca
    24961 gctgccatgc ccgccagata aacggagaac gcaatatgca actgcgcttc gctggcattg
    25021 agatcgtcgg cgatgcgcgg taaaccaacg aggtacatat caatcccggc gggataaagt
    25081 aaaaccaggg caaaactaca aattaaaaag cgggacatgg gctaaagcgt cctaatagta
    25141 aggtgacgca aagcatacgt ggtgaaggag aatcaggcga gttgccattt ggacataagt
    25201 gatttcctga gaggcaagaa gccagcgaat ggctggcttc ttgaaggatt tagccaaatt
    25261 taggtaacac gttaaagacg ttgccgaacc agcacaaaat cacggttata ccaaagagaa
    25321 tgacaatcgc cggaataaga ttgccgcccc agaccgtgaa catctgattg ggaaacttct
    25381 tgcgagcttt gattgcaagc actgcgggaa taatgaccgc ccagatggtg gcgcacagcc
    25441 cggcaccgcc gatcccgtaa ataaagccgt tcgggaagat cagatacaac aacgcaggtg
    25501 gcaggaaggt taacagcacg gttttgaaac gcccgccgtg ggagttatca atcttaaaca
    25561 ggtccgccag ataatcgaac aaccccagcg tgacaccaaa gaacgaactg gcaacagcta
    25621 agttagagaa caccagcagg caaaactcga taatgccgtg ctgtttggtg ccgaggaacg
    25681 atttcaccag cgaatcaacg ttgccgcctg aggagattat cgccttaaag ctttcgcgcg
    25741 gaatattccc catcgtgcaa tagagccaga agagataaat caccagcgcc agcagcgaac
    25801 caaataccac gcttttgatt aacttatctt tgcgttttcc atagcaaata atcaggctgg
    25861 gaatattgcc gtggaaacca aatgacgcca gacacaccgg caaagccata aagatatacg
    25921 ggaagtaaga cgttcccgca gtggtgctgg tggcatcgcg caggatggag taatcgacct
    25981 ggaagaagaa agaaccaaac acgatcacaa aggagataat cttcagcccg aggaacaatg
    26041 aggtaatgcg actggaggct aacgaactta tccacaatac gctggcaacg aaaatggctg
    26101 tgcagatccc gacaatacgt ggattagcgt gatagcccaa attcattgat atcgtttcac
    26161 taatgatcgc accattagca gagatatagg cataagtgag gatatagaga acgaaggcaa
    26221 cggtaatacc gctgataatg ttccaggtgt taccgattaa atctttggtg atggtgttaa
    26281 aactggagcc gacaggataa tttaaatttg cttctaataa caataacccg gaatgaagca
    26341 ttgaaaacca ggcaatgata aggataaagg caccccagaa aaaccaggca ccggcaagat
    26401 caacaggtaa agcaaacata cctccaccaa ttactgtacc tgctataacc ataacacccc
    26461 aaaatgcaga gtgctttttt tcagcttgat cagtcatgat gccaccttta gaggaaggct
    26521 atttttgtta ttgaggatgt agggtaagag agtggctaac atccttatag ccactctgta
    26581 gtattaatta aacttctttc agttttgcgg tgaagtgacg caatactttt ggttcgtagg
    26641 taaaggttaa tcctttaata ttcgccgcgt tctctttcac atgtttaaag gcttcaataa
    26701 tgaagtccat atgtgtttga gtatatgttg cgcgcggaat ggttaaacgc agcagttcag
    26761 ccgggcatgg cagttgttta ccggttttcg gatcgcggcc taacaggaaa gagccaattt
    26821 ctaccgcacg gataccggcg actttataca gctcgcacgc cagcgcctgt gccgggaact
    26881 ggtctgccgg gatatgcggc agcagtttac cggcatcaac gaatgccgcg tgaccgcccg
    26941 cctgctggca gacaacgcca atctcttcca gaccatcgac cagatactgt acctgcgcga
    27001 tacgataagc cagccagtcg agattcatgc cgtcatacag acctaccgcc agacgctcca
    27061 tcgcgccgcc ttccaggccg ccatatgtcg ggaacccttc ctgcaccacg caaagggttc
    27121 tgcactcggt gtacacatca aagaagctgt cgtctttcat gcacagcaag ccgcccatcg
    27181 gtaccatcgc atctttcttg gcggacatcg ccagcatatc ggcatatttg taggtttcgc
    27241 gggtgatctg ctcgatggtc cagtctttgt attctgcttc acgctgcttg atgaaatagg
    27301 cgttttcagc aaagcgtgcg gagtccatta ccaccggaat atcgtatttc ttcgcgatgc
    27361 tgtacatcgc ttttaagttt gccagtgaaa ccggctgacc acctgcagag ttactggtga
    27421 tggttgcaac gatatacggc acgttattcg ggccaacttc ttcaataccg cgttctaatc
    27481 cctcaaggtc aaagttacct ttaaagtcgt aacgcacgcc agtatcgaag gcttctttga
    27541 tatagacgtt acgcacggta cagccgttaa tctggctatg gccctgcgtg gtatcaaaga
    27601 aatagttaga gaatgccacc attttgctgc gatccaggcc tttttcctgc tcgcgttttt
    27661 taatcagtac cggaatataa atttgctctg cgccacggcc ctggtgagtc ggaatggtgt
    27721 attgataacc aaagatattt ttcactgact cggctaacgc atagtagctg cggctgccgc
    27781 tgtaggcttc gtcgccgcgc atcatcgctg cctgcatact ttgggttacc gccccggtgc
    27841 cgctgtcggt cagtaaatcg ataaacacat cttcgctatc cagcaggaac gggttcatac
    27901 cggatttaat aattgcttct tcacgataag cgcgagtagt acgttttact ggctcaataa
    27961 cacgaatgcg gaacggttca gggagatgtt taaagttttc cattacataa tccttcattt
    28021 attttaatta cagtgatccc tgtgaatatt acatctgcaa tatccattaa ttcgccatgt
    28081 atatatacaa gcaccatcat ttactaaaat aagtaaataa acgagagatg gcctttgcaa
    28141 aaggcaaaat taagatatag caaactaatc gttcacattg aatctaatcg gtttggctct
    28201 ggtgatggct acagaagggt aaattaaggg cggtgatcga caatcttatt gtcgatattg
    28261 aaccattttg aggtcacaca tatatgtaag atattcataa tgcacttatc ctcgaaagac
    28321 acgaggctac agtataatac tcaagaattt tgtctattct aaaattgttt aaatgtgaat
    28381 cgaatcacaa tcgttcgggg agcaatatta aatgcttttt tttaagatta taaattacgt
    28441 aagaatttta atgtttaaaa aaagggttga ggaataacag gagtgctaac aatgaagcga
    28501 agccgcatct gacagtcaga atacggcttc gtaagcgcgg ttacttacca atacagaagc
    28561 tggagaaaat ccgccccagc aggtcgtctg aagtaaattc cccggtgatt tcgcttaagt
    28621 tctgctgtgc cagacgcaac tcttccgcca gcagttcacc tgcccaggct cccaacagtt
    28681 gcgctttgcc ctgttgtaga tgttccgctg cctgtgccag cgcctgtagg tggcgacgac
    28741 gcgccaggaa gccgccttcc atgttggtgt caaagcccat gctctgtttg agatggttac
    28801 gcagcacgtc cacgccttca ccagtccttg ccgagagacg aattaacgcg tgaccgttca
    28861 cttcactcat tcccagcgtt tcgccggtga tatcggcttt attgcgcacc acggtgatcg
    28921 gcagtttcgc tggcagacgg gcaataaatt ccggccagat ctctgccgga tccacggcgt
    28981 ctgttgtggt gccatcgacc ataaacagca cgcggtcggc ctgttcaatt tcctgccacg
    29041 cgcgctcgat accaatacgt tccacttcgt cactggcttc acgtagccca gcggtatcga
    29101 tgatatgcag cggcattccg tcaatgtgga tatgctcacg cagcacgtca cgtgtggttc
    29161 cggcgatatc agttacgatt gctgcttcac ggcccgccag cgcgtttaac aggctcgatt
    29221 taccggcgtt aggacgtccg gcaatcacca ccttcatccc ttcgcgcaac aaactaccct
    29281 gacgtgcttc agcacgcact gcatcgagat cggcaatcac gtcattaagc tgggcttcaa
    29341 ttttgccgtc ggagaggaaa tcgatctctt catcagggaa atcaattgcc gcttcgacgt
    29401 aaatgcgcaa gtgggtgagg gcttccacca gatggttgac ccgtgcggag aatgcgcctt
    29461 gcagcgagtt aagtgccgaa cgggccgcct gttccgaact ggcgtcgata agatcggcaa
    29521 tcgcctcggc ctgggctaag tcgagtttat cgttaagaaa cgcgcgttcg gaaaactcac
    29581 cagggcgagc aatccgcagg ccgggaatgg tcagaatgcg ttttaacagc aggtcgagga
    29641 tcaccggacc gccatgacct tgcagttcca gcacatcttc gccggtgaac gagttcgggc
    29701 cagggaacca tagcgcaatc ccctgatcga gcacgctgcc gtcggcgtct ttaaacggaa
    29761 gataatcggc gtagcgcggc ttaggtagtt tacccagcac ggtttcggca acttcacggg
    29821 ctttgaagcc ggagatgcgc aggatgccaa cgccgccacg tcccggaggc gtggcctggg
    29881 ctacgatagt gtcattatcg ctcatgatgt tcctgttgct ttgtgtggcg gatgcgcggt
    29941 gcttatccgc cctacgaaaa gaaaaaaggc ggtcaactga ccgcccttat tttagcgaaa
    30001 actcaccgaa tcaggatttt ttcttctcgc ggctatgcag gccacgtttt tccagaccac
    30061 ggtaaatcag ctgctgctga ataatggtta ccaggttgct gacgatatag tacagcacca
    30121 gacctgacgg gaaccacagg aagaacacgg tgaagatgac cggcataaag gtcatgatct
    30181 tctgctgcat cgggtcggtc actgtggtcg gcgacatctt ctgaatgaag aacatcgtta
    30241 cgcccatcag gatcggcaag atgtagtacg ggtcctgtgc cgacaggtcg tggatccaca
    30301 gtgcaaacgg tgcctgacgc agttcaacgg aacccatcag catgtagtac aacgccagga
    30361 agattggcat ctggatcagc agcgggaagc agccgcccag cgggttaacc ttctcagctt
    30421 tgtacagcgc catcatttcc tggctgatac gctgtttgtc atcgcccaga cgctcacgca
    30481 ttgcctgaat cttcggctgc agcatacgca tcttcgccat ggaggtgtac tgcgctttgg
    30541 tcagcgggta catgatgcca cgaacgataa aggtgatgat gataatggag aagccccagt
    30601 tacccacaaa gctatggatc catttcagca gtttgaacag cggctgagag atgaaccaca
    30661 accaaccgta atcaacggtc agatccaggt gcggagcaac agctgccatt ttgtcctgga
    30721 tttccgggcc aacccacagg gtgctgttca tcgcgccagt ctgaccaggc tgaaccagta
    30781 ccggctgaga tttatagccg atagcggcga tgccgttacc cagattagcg gtatagaagt
    30841 tgttggtacc gtcgttatgc gggatccacg ccgtcgcgaa atactgttgc agcatcgcca
    30901 cccaaccacc tttcgaagag atgttcaggt tttcgttatc ggcaatggta tcgaacttgt
    30961 atttctcgta cttctcgtca ggcgtggagt acgccgcgcc gcggaaggtg tgcagtgcga
    31021 agttgctgct tccggtatcg agatgcggtg gcagagtgat ggattgcttc aactgaccaa
    31081 aggtggagat ttccagcggt ttctcgccag cgttctgcac gttgtagttg acgttgacag
    31141 cgtaatcacc acgtttcagg acaaacgttt tggtaaacgt gttgcctgcc gcgtcggtat
    31201 acgtcatcgg cacctgcagt tcgttttgac cttcagccag cacataagcg tctttttcaa
    31261 cgttatacag cggacgcggg ccgttagccg ggttatccgg gccatcacga ccggtcagac
    31321 cgctctgtgc ctgataaata aactgcggtg aagtttccag cagctggaac ggctgggtag
    31381 agttcagctc tttcgggtaa gcaggcagca gagcttgctc aacatcacca ccacgggtgt
    31441 tgatggtcag atcaagcacg tcggtcttaa ccgagatcag tttcccctgg ccactggccg
    31501 gtacgccctg gtcggcggcg ctacccgctg cggtggtcgt tgtctgcgtg gtctgttggg
    31561 cctgaggttg cgggttttta tcctgctccc aggcttgcca gatcatgaaa gacacgaaca
    31621 gcaaagcgat gactaaaaga ttgcgttgcg aatccatcgt tagtgttctc tggtatcaaa
    31681 tggtccgggc gggacgggat cgtcaccacc agggtgtaaa gggtggcatt ttaatacgcg
    31741 tttcaccgtc aaccaactgc cttttatcac tccaaacctg cgcaatgcct caattccgta
    31801 gcttgaacag gttggagtga aacgacaatg cggcccgagt agcggactaa tcaggcgttg
    31861 atagacccga atgagggcta tcaggacccg cgagccaggc gacagtggcg gcgccataat
    31921 ttttccaacg cttccgagag agcacggtta tcgaggtcgg caaccccttt tttcgccacc
    31981 accacgaaat ccatagccgg gagttcatgt tggcgcagac ggaagctttc acgcgtcaga
    32041 cgtttaatcc gattgcgttc atgggcgcgt cgaacgtttt tcttggcgac tgtaagaccg
    32101 atacggggat gccccagcga attcaggcgg ccgagaatgg taatttgcgg cgtgccagcc
    32161 cgttgtggct gctggaagac gaatgtgaat tgactgggag ttaacaagcg taactccctg
    32221 ggaaatgcga gcttaaccac tcaggggtta gctttattac ttagaaacgg tcagacgagc
    32281 gcggccttta gcacgacgac gtgccagaac ctgacgacca tttttagtag ccatacgagc
    32341 acggaagccg tgagaacggt tgcgcttcag tacagacggt tgaaaagtgc gtttcatggc
    32401 gatttctacc taaacttgaa taaattcaat ggctttattg gatatccgcc gaaaaatgaa
    32461 acgatggaca ccgaagccat gggtgattaa agaggccgga ttgtaataat tgtacactcc
    32521 ggagtcaatt ctctttcctt atttaccgcg cttttccgca ccttttcgca gggaaaatgt
    32581 acgacctcac accagtggaa accagcatgg cgcgccgggt ggaggattat acgggctgat
    32641 gggtaaagcg caaggatcgt cctggatctt tattagatcg attaagccaa tttttgtcta
    32701 tggtcattaa attttccaat atgcggcgta aatcgtgccc gcctcgcggc aggatcgttt
    32761 acacttagcg agttctggaa agtcctgtgg ataaatcggg aaaatctgtg agaaacagaa
    32821 gatctcttgc gcagtttagg ctatgatccg cggtcccgat cgttttgcag gatcttgatc
    32881 gggcatataa ccgcagacag cggttcgtgc gtcaccctca agcagggtct tttcgacgta
    32941 cgtcaacaat catgaatgtt tcagccttag tcattatcga cttttgttcg agtggagtcc
    33001 gccgtgtcac tttcgctttg gcagcagtgt cttgcccgat tgcaggatga gttaccagcc
    33061 acagaattca gtatgtggat acgcccattg caggcggaac tgagcgataa cacgctggcc
    33121 ctgtacgcgc caaaccgttt tgtcctcgat tgggtacggg acaagtacct taataatatc
    33181 aatggactgc taaccagttt ctgcggagcg gatgccccac agctgcgttt tgaagtcggc
    33241 accaaaccgg tgacgcaaac gccacaagcg gcagtgacga gcaacgtcgc ggcccctgca
    33301 caggtggcgc aaacgcagcc gcaacgtgct gcgccttcta cgcgctcagg ttgggataac
    33361 gtcccggccc cggcagaacc gacctatcgt tctaacgtaa acgtcaaaca cacgtttgat
    33421 aacttcgttg aaggtaaatc taaccaactg gcgcgcgcgg cggctcgcca ggtggcggat
    33481 aaccctggcg gtgcctataa cccgttgttc ctttatggcg gcacgggtct gggtaaaact
    33541 cacctgctgc atgcggtggg taacggcatt atggcgcgca agccgaatgc caaagtggtt
    33601 tatatgcact ccgagcgctt tgttcaggac atggttaaag ccctgcaaaa caacgcgatc
    33661 gaagagttta aacgctacta ccgttccgta gatgcactgc tgatcgacga tattcagttt
    33721 tttgctaata aagaacgatc tcaggaagag tttttccata ccttcaacgc cctgctggaa
    33781 ggtaatcaac agatcattct cacctcggat cgctatccga aagagatcaa cggcgttgag
    33841 gatcgtttga aatcccgctt cggttgggga ctgactgtgg cgatcgaacc gccagagctg
    33901 gaaacccgtg tggcgatcct gatgaaaaag gccgacgaaa acgacattcg tttgccgggc
    33961 gaagtggcgt tctttatcgc caagcgtcta cgatctaacg tacgtgagct ggaaggggcg
    34021 ctgaaccgcg tcattgccaa tgccaacttt accggacggg cgatcaccat cgacttcgtg
    34081 cgtgaggcgc tgcgcgactt gctggcattg caggaaaaac tggtcaccat cgacaatatt
    34141 cagaagacgg tggcggagta ctacaagatc aaagtcgcgg atctcctttc caagcgtcga
    34201 tcccgctcgg tggcgcgtcc gcgccagatg gcgatggcgc tggcgaaaga gctgactaac
    34261 cacagtctgc cggagattgg cgatgcgttt ggtggtcgtg accacacgac ggtgcttcat
    34321 gcctgccgta agatcgagca gttgcgtgaa gagagccacg atatcaaaga agatttttca
    34381 aatttaatca gaacattgtc atcgtaaacc tatgaaattt accgtagaac gtgagcattt
    34441 attaaaaccg ctacaacagg tgagcggtcc gttaggtggt cgtcctacgc taccgattct
    34501 cggtaatctg ctgttacagg ttgctgacgg tacgttgtcg ctgaccggta ctgatctcga
    34561 gatggaaatg gtggcacgtg ttgcgctggt tcagccacac gagccaggag cgacgaccgt
    34621 tccggcgcgc aaattctttg atatctgccg tggtctgcct gaaggcgcgg aaattgccgt
    34681 gcagctggaa ggtgaacgga tgctggtacg ctccgggcgt agccgttttt cgctgtctac
    34741 cctgccagcg gcggatttcc cgaacctcga tgactggcag agtgaagtcg aatttaccct
    34801 gccgcaggca acgatgaagc gtctgattga agcgacccag ttttcgatgg cgcatcagga
    34861 cgttcgctat tacttaaatg gtatgctgtt tgaaaccgaa ggtgaagaac tgcgcaccgt
    34921 ggcaaccgac ggccaccgtc tggcggtctg ttcaatgcca attggtcaat ctttgccaag
    34981 ccattcggtg atcgtaccgc gtaaaggcgt gattgaactg atgcgtatgc tcgacggcgg
    35041 cgacaatccg ctgcgcgtgc agattggcag caacaatatt cgcgcccacg ttggcgactt
    35101 tatcttcacc tccaaactgg tggatggtcg cttcccggat taccgccgcg ttctgccgaa
    35161 gaatccggac aaacatctgg aagctggctg cgatctgctc aagcaggcgt ttgcccgtgc
    35221 ggcaattctc tctaacgaga aattccgcgg cgtgcgcctg tatgtcagcg aaaaccagct
    35281 gaaaatcacc gccaacaacc cggaacagga agaagcggaa gagatcctcg acgttaccta
    35341 tagcggtgcg gagatggaaa tcggcttcaa cgtcagctat gtgctggatg ttctgaacgc
    35401 gctgaaatgc gaaaacgtcc gcatgatgct gaccgattcg gtttccagcg tgcagattga
    35461 agatgccgca tcacagtcgg ctgcctatgt tgtcatgcca atgagactgt aatgtccctc
    35521 acccgcttgt tgatccgcga tttccgcaac attgaaaccg cggatctcgc tttatctccc
    35581 ggctttaact ttctggtagg tgccaacggc agtggcaaaa ccagcgtgct ggaagccatc
    35641 tatacgctcg gccatggtcg ggcgtttcgc agtttgcaga ttggtcgcgt cattcgccat
    35701 gagcaggagg cgtttgttct ccacgggcga ttacagggcg aagagcgcga gacagcgatt
    35761 ggcttaacca aagacaaaca gggcgacagc aaagtccgca tcgacggtac agacgggcat
    35821 aaggtcgcgg aactggcgca cctgatgcca atgcagttga taacgccaga agggtttact
    35881 ttactcaacg gcggccccaa atacagaaga gcattcctcg actggggatg ctttcacaac
    35941 gaacccggat ttttcaccgc ctggagcaat ctcaagcgat tgctcaagca gcgcaatgcg
    36001 gcgctgcgcc aggtgacacg ttacgaacag ctacgcccgt gggataaaga actgatcccg
    36061 ctggcggagc aaatcagcac ctggcgcgcg gagtatagcg ccggtatcgc ggctgatatg
    36121 gccgatactt gtaagcaatt tctccctgag ttttctctga ctttctcttt ccagcgcggc
    36181 tgggagaaag agacagaata tgctgaggtg ctggaacgta attttgaacg cgatcgccag
    36241 ctaacctaca ccgcgcatgg cccgcataaa gcggacttac gcattcgcgc cgacggtgcg
    36301 ccggtggaag ataccttatc gcgtgggcag cttaagctgt tgatgtgcgc cttacgtctg
    36361 gcgcaaggag agttcctcac ccgtgaaagc gggcggcggt gtctctacct gatagatgat
    36421 tttgcctctg agcttgatga tgagcgtcgc gggctgcttg ccagccgctt aaaagcgacg
    36481 caatcacagg tctttgtcag cgcgatcagt gctgaacacg ttatagacat gtcggacgaa
    36541 aattcgaaga tgtttaccgt ggaaaagggt aaaataacgg attaacccaa gtataaatga
    36601 gcgagaaacg ttgatgtcga attcttatga ctcctccagt atcaaagtcc tgaaagggct
    36661 ggatgcggtg cgtaagcgcc cgggtatgta tatcggcgac acggatgacg gcaccggtct
    36721 gcaccacatg gtattcgagg tggtagataa cgctatcgac gaagcgctcg cgggtcactg
    36781 taaagaaatt atcgtcacca ttcacgccga taactctgtc tctgtacagg atgacgggcg
    36841 cggcattccg accggtattc acccggaaga gggcgtatcg gcggcggaag tgatcatgac
    36901 cgttctgcac gcaggcggta aatttgacga taactcctat aaagtgtccg gcggtctgca
    36961 cggcgttggt gtttcggtag taaacgccct gtcgcaaaaa ctggagctgg ttatccagcg
    37021 cgagggtaaa attcaccgtc agatctacga acacggtgta ccgcaggccc cgctggcggt
    37081 taccggcgag actgaaaaaa ccggcaccat ggtgcgtttc tggcccagcc tcgaaacctt
    37141 caccaatgtg accgagttcg aatatgaaat tctggcgaaa cgtctgcgtg agttgtcgtt
    37201 cctcaactcc ggcgtttcca ttcgtctgcg cgacaagcgc gacggcaaag aagaccactt
    37261 ccactatgaa ggcggcatca aggcgttcgt tgaatatctg aacaagaaca aaacgccgat
    37321 ccacccgaat atcttctact tctccactga aaaagacggt attggcgtcg aagtggcgtt
    37381 gcagtggaac gatggcttcc aggaaaacat ctactgcttt accaacaaca ttccgcagcg
    37441 tgacggcggt actcacctgg caggcttccg tgcggcgatg acccgtaccc tgaacgccta
    37501 catggacaaa gaaggctaca gcaaaaaagc caaagtcagc gccaccggtg acgatgcgcg
    37561 tgaaggcctg attgcggtcg tttccgtgaa agtgccggac ccgaaattct cctcccagac
    37621 caaagacaaa ctggtttctt ctgaggtgaa atcggcggtt gaacagcaga tgaacgaact
    37681 gctggcagaa tacctgctgg aaaacccaac cgacgcgaaa atcgtggttg gcaaaatcat
    37741 cgatgctgcc cgtgcccgtg aagctgcgcg tcgtgcgcgt gaaatgaccc gccgtaaagg
    37801 tgcgctcgac ttagcgggcc tgccgggcaa actggcagac tgccaggaac gcgatccggc
    37861 gctttccgaa ctgtacctgg tggaagggga ctccgcgggc ggctctgcga agcaggggcg
    37921 taaccgcaag aaccaggcga ttctgccgct gaagggtaaa atcctcaacg tcgagaaagc
    37981 gcgcttcgat aagatgctct cttctcagga agtggcgacg cttatcaccg cgcttggctg
    38041 tggtatcggt cgtgacgagt acaacccgga caaactgcgt tatcacagca tcatcatcat
    38101 gaccgatgcg gacgtcgacg gctcgcacat tcgtacgctg ctgttgacct tcttctatcg
    38161 tcagatgccg gaaatcgttg aacgcggtca cgtctacatc gctcagccgc cgctgtacaa
    38221 agtgaagaaa ggcaagcagg aacagtacat taaagacgac gaagcgatgg atcagtacca
    38281 gatctctatc gcgctggatg gcgcaacgct gcacaccaac gccagcgcac cggcattggc
    38341 tggcgaagcg ttagagaaat tggtgtctga gtacaacgcg acgcagaaaa tgatcaaccg
    38401 catggagcgt cgttatccga aagcaatgct gaaagagctt atctatcagc cgacgctgac
    38461 ggaagccgac ctctctgatg agcagaccgt tacccgctgg gtgaacgcgc tggtcagcga
    38521 actgaacgac aaagaacagc acggcagcca gtggaagttt gatgtccaca ccaatgccga
    38581 acaaaacctg tttgaaccga ttgttcgcgt gcgtacccac ggtgtggata ctgactatcc
    38641 gctggatcac gagtttatca ccggtggcga atatcgtcgt atctgcacgc tgggtgagaa
    38701 actgcgtggc ttgctggaag aagatgcgtt tatcgaacgt ggcgagcgtc gtcagccggt
    38761 agccagcttc gagcaggcgc tggactggct ggtgaaagag tcccgtcgcg gcctctccat
    38821 ccagcgttat aaaggtctgg gcgagatgaa cccggaacag ctgtgggaaa ccaccatgga
    38881 cccggaaagc cgtcgtatgc tgcgcgttac cgttaaagat gcgattgctg ccgaccagtt
    38941 gttcaccacg ctgatgggcg acgccgttga accgcgccgt gcgtttattg aagagaacgc
    39001 cctgaaagcg gcgaatatcg atatttaatg gcgctaacca tgcgagcgtg cctgatgcgc
    39061 tacgcttatc aggcctacga aaatcttgca atgtattgaa ttttcatgat tttgtaggcc
    39121 ggataaggcg ttcacgccgc atccggcaag aagctataag aaaagggcgg agatcatctc
    39181 cgcccttttt atttctgcaa tccgcaatta tcatcccgca aatctatact ttttgggtca
    39241 acacgtaacg ctttacaggt gattatcatg gggctttttg atgaagttgt cggtgccttt
    39301 ctgaaaggcg atgcggggaa atatcaggct attttaagtt gggttgagga gcagggcggc
    39361 attcaggtgc tgctggaaaa actgcaaagt ggcggcttag gggccattct ctcaacctgg
    39421 ctgagtaatc aacagggcaa tcaatcggtt agtggcgagc aactggaatc ggcgctcggc
    39481 acaaatgcgg tgtccgatct cgggcaaaaa cttggcgtgg ataccagtac agcttccagt
    39541 ttactggcag agcaattgcc gaagattatt gatgcgctct caccgcaagg tgaagtgtca
    39601 ccacaagcca ataacgatct gctttccgca ggtatggaac tgctgaaagg gaaactcttc
    39661 cgctaagcaa atggggagca tgcaacccgc gttgcatccc ccttttccct cgggtgttgt
    39721 actgattttt gagcggaatc gcgttagcat gggtcaggaa ccaatctacc tggggaactc
    39781 atggctatca aactcattgc tatcgatatg gatggcaccc ttctgctgcc cgatcacacc
    39841 atttcacccg ccgttaaaaa tgcgattgcc gcagctcgcg cccgtggcgt gaatgtcgtg
    39901 ctaacgacgg gtcgcccgta tgcaggtgtg cacaactacc tgaaagagct gcatatggaa
    39961 cagccgggcg actactgcat tacttataac ggcgcgctgg tacagaaggc cgctgatggt
    40021 agcaccgtgg cgcaaactgc tctcagctat gacgactatc gtttcctgga aaaactctct
    40081 cgcgaagtcg gttctcattt ccacgccctg gaccgcacca cgctgtacac cgccaaccgt
    40141 gatatcagct actacacggt gcatgaatcc ttcgttgcca ccattccgct ggtgttctgc
    40201 gaagcggaga aaatggaccc caatacccag ttcctgaaag tgatgatgat tgatgaaccc
    40261 gccatcctcg accaggctat cgcgcgtatt ccgcaggaag tgaaagagaa atataccgtg
    40321 ctgaaaagtg cgccgtactt cctcgaaatc ctcgataaac gcgttaacaa aggtacgggg
    40381 gtgaaatcac tggccgacgt gttaggtatt aaaccggaag aaatcatggc gattggcgat
    40441 caggaaaacg atatcgcaat gattgaatat gcaggcgtcg gtgtggcgat ggataacgct
    40501 attccttcgg tgaaagaagt ggcgaacttt gtcaccaaat ccaaccttga agatggcgtg
    40561 gcgtttgcta ttgagaagta tgtgctgaat taatcagtgg acgggcaaac agcataaacg
    40621 cgtttgcccg cttactgatt attggtttac tgcatataca accagaaatt gcagcggtgc
    40681 agtaatgatg tccagcgtaa cagtcactgg cagtaatgct gcggaaacac catccggaat
    40741 gcctctgggt gaatagtatt cataaaatcg cactttaaac ggatgataga acgccatcac
    40801 ctttgagtaa tccatgtttt tatttttctc atggattgtg ccttttaggt tttccagtga
    40861 cctggtacag tgttgaacac cacaggcaaa accataatgc gttgccagtg ccttttcttc
    40921 ttcgttattc cagtagtagt aaagcgatat tgtgccggta aatttctttt tccccgcatt
    40981 taaaacaaat cttgcgtcat cggcaacctg aatattgtgc cggttaagtg ctggatcatt
    41041 gagcatctta acgatgtcat cggcaccatc agtgataagg taatccagcg attcccctac
    41101 aaaaacatag ccttttgttc cattactatc ttttgcctgc gatagcccag tgatggtgtc
    41161 tgactgcgat tgatactttc ctgaactacc ctttgcggta tgggaaacca gagcatacga
    41221 gatacagcct gaaagcatca gcgccagtgg gaataaaccg gcagccttaa aaaatccctt
    41281 aaaattcaac ttcatgatta atcctttaaa cgagtagcta agccgttgaa aacatactct
    41341 tctttccctc ctgtttagcg cctgatatcc cttttcagat ttctgcccga cgcatgtcat
    41401 ttttttatgc attgttcttt ttgtgatcta aattgtagta caacaatata agtttgtact
    41461 acattacgca cacggcaaac gcgaacgtca tcacgctagt actacaaagt tgccgcgtta
    41521 tgcatcgatc ggggtaaagt agagaagaac atacagagca caaggactct ccatgactct
    41581 caataaaacc gatcgcattg tcattacgct gggtaaacag atcgttcacg gcaaatacgt
    41641 acctggctcg ccacttccgg ctgaggcgga gctctgtgaa gagtttgcaa cctcgcgcaa
    41701 catcatccgt gaggtgttcc gttcgttgat ggcgaagcgg ctgattgaaa tgaaacgtta
    41761 tcgcggcgcg tttgtggcac cgcgtaacca gtggaattac ctcgacactg acgtactgca
    41821 atgggtgctg gaaaacgact acgacccacg gcttatcagt gccatgagcg aagtgcgaaa
    41881 tctggtggaa ccggcgattg cccgttgggc agcagagcgc gcgacctcca gcgatctggc
    41941 gcagattgaa tcggcgctga acgagatgat tgccaacaat caggaccgcg aagcgtttaa
    42001 cgaagcggat attcgctacc acgaggcggt gctgcagtcg gtgcataacc cggtgttaca
    42061 gcaacttagc attgcgatca gttcgctgca gcgggcggtt tttgaacgaa cctggatggg
    42121 cgatgaggcc aacatgccgc aaacgctcca ggaacataag gcgctgttcg atgcgatacg
    42181 acatcaggac ggcgatgcgg cagagcaggc ggcgcttacc atgatcgcca gctcgacacg
    42241 aaggttaaag gaaatcacat gacagctcgc tacatcgcaa ttgactgggg atcgaccaat
    42301 ctgcgtgcct ggctttatca gggcgaccac tgcctggaca gcaggcaatc agaagcaggc
    42361 gtcacgcgcc tgaacggaaa atctccggct gcggtgttag cagaagtcac gaccgactgg
    42421 cgtgaagaga atacgccagt ggtgatggca ggaatggtcg gcagcaatgt cggctggaaa
    42481 gttgcaccgt atttatctgt tcctgcccgt ttttcgtcta ttggcgatca attaacgtcg
    42541 gttggcgaca atatctggat tattcccgga ttatgcgtct ctcatgacga taaccacaat
    42601 gtgatgcgcg gcgaagaaac acaattgatc ggcgcgcgca ctctggctcc ttcctctctt
    42661 tatgtcatgc ccggaaccca ttgcaaatgg gtgcaggccg atagccagca aatcaacgat
    42721 tttcgcaccg tgatgaccgg tgaattacat catttactgt taaatcactc attgattggc
    42781 gcaggtttgc cgccgcagga aaactctgcc gatgccttcg cggctggcct tgagcgtggc
    42841 cttaatgcgc ccgccatatt gccgcagctt tttgaagttc gcgcctcgca tgtgctggga
    42901 acacttcccc gcgaacaggt cagcgaattt ctctctggtt tgttgattgg cgcagaggtc
    42961 gccagtatgc gcgactatgt ggcccatcaa cacgccatca cccttgtcgc cggaacatcg
    43021 ctgaccgcgc gctaccagca agcctttcag gcgatgggtt gcgacgtgac agcggtagca
    43081 ggcgacacgg catttcaggc tggtataagg agcatcgctc atgcagtggc aaactaaact
    43141 cccgctgatc gccattttgc gcggcattaa gcccgacgag gcgctggcgc atgttggcgc
    43201 ggtgattgac gccgggttcg acgcggttga aatcccgctg aattccccac aatgggagca
    43261 aagcattccc gccatcgttg atgcgtatgg cgacaaggcg ttgattggcg caggtacggt
    43321 actgaaacct gaacaggtcg atgcgctcgc caggatgggc tgtcagctca tcgttacgcc
    43381 caatatccat agtgaagtga tccgccgtgc ggtgggctac ggcatgaccg tctgccccgg
    43441 ctgcgcgacg gcgaccgaag cctttaccgc gctcgaagcg ggcgcgcagg cgctgaaaat
    43501 atttccgtca tcggcttttg gtccgcaata catcaaagcg ttaaaagcgg tattgccatc
    43561 ggacatcgca gtctttgccg ttggcggcgt gacgccagaa aacctggcgc agtggataga
    43621 cgcaggttgt gctggggcgg gcttaggcag cgatctctat cgcgccgggc agtccgtaga
    43681 acgcaccgcg cagcaggcag cagcatttgt taaggcgtat cgagaggcag tgcaatgaaa
    43741 atcaccaaaa ttaccacgta tcgtttacct ccccgctgga tgttcctgaa aattgaaacc
    43801 gatgaaggcg tggtcggttg gggcgagccc gtgattgaag gccgcgcccg tacggtggaa
    43861 gcggcagttc acgagctggg tgactatttg attggtcagg atccttcgcg catcaatgac
    43921 ttatggcaag tgatgtatcg cgccggattt tatcgtggcg gtccaatcct gatgagcgcc
    43981 attgccggga tcgaccaggc gttatgggat atcaaaggta aagtgctgaa tgcgccggtc
    44041 tggcaactga tgggcggcct ggttcgcgac aaaattaaag cctacagttg ggtcggcggc
    44101 gatcgtccgg cggatgtgat cgacggtatt aaaaccctgc gcgaaatcgg cttcgatacc
    44161 ttcaaactga acggttgtga agaactgggg ctaattgata actcccgcgc ggtagatgcg
    44221 gcagtcaaca ccgtggcaca aattcgtgaa gcttttggca atcagattga gtttggtctt
    44281 gatttccacg gtcgcgtcag cgcgccaatg gcgaaagtgc tgattaaaga actggagccg
    44341 tatcgcccgc tgtttattga agagccggtg ctggcggaac aagccgaata ctacccgaaa
    44401 ttggcggcac aaacgcatat tccactggcg gcgggtgagc gcatgttctc acgtttcgat
    44461 tttaaacgcg tgctggaggc aggcggtatt tcgattctac aaccggatct ctcccatgca
    44521 ggcggtatta ccgaatgcta caaaattgcc ggaatggcag aagcctatga cgtgaccctt
    44581 gcgccgcact gtccgctcgg accgattgca ctggcggctt gtctgcatat cgactttgtt
    44641 tcctataacg cggtacttca ggaacaaagt atgggaattc attacaacaa aggcgcggag
    44701 ttactcgact ttgtgaaaaa caaagaagac ttcagcatgg ttggcggctt ctttaaaccg
    44761 ctaacgaaac cgggcttagg cgtggaaatc gacgaagcta aagttattga gttcagtaaa
    44821 aatgccccgg actggcgtaa tccgctctgg cgtcatgtag ataacagcgt agcagagtgg
    44881 taattcctgc cacgtaagcc cctcatcggg cactaaaaca gcaatacaaa aatataaccc
    44941 tctgtaaatt acagggcatg gtgagcggct tcgctatgcc caaaatctgg ataaagctcg
    45001 ctatggatat tcccgttaat gcagcaaagc cggggcgtcg gcgttatctg acgctggtga
    45061 tgatctttat tacggtggtc atttgttatg ttgaccgcgc taacctggcc gtggcttccg
    45121 cccatattca ggaagagttc ggcattacga aagcggaaat gggctatgta ttttcggcct
    45181 tcgcctggct ttatacgcta tgccagatcc ccggcggttg gtttttagat cgcgtaggtt
    45241 ctcgcgtgac ttattatatt gcgatatttg gttggtcagt ggcgacttta ttccagggct
    45301 ttgccacggg attaatgtca ttaattggtc tgcgtgcgat aaccggtatt ttcgaagcgc
    45361 ccgctttccc gaccaataac cggatggtga ccagctggtt cccggaacat gaacgcgctt
    45421 ccgccgttgg tttttatacg tctggtcagt ttgtcggtct ggcgtttttg actccgctgc
    45481 tgatctggat tcaggagatg ttgagctggc actgggtgtt cattgtcact ggtggtatcg
    45541 gcattatctg gtcgctgatt tggtttaagg tttatcagcc gccgcgcctg accaaaggta
    45601 tcagcaaagc tgaactggat tacattcgtg atggcggcgg tctggtggat ggtgatgcgc
    45661 cggtgaagaa agaggcgcgt cagccgttaa cagccaaaga ctggaaactg gtgttccatc
    45721 gtaaactgat cggcgtttat cttgggcaat ttgcggtggc ttctacactg tggtttttct
    45781 taacctggtt cccgaactat ttaacccagg aaaaaggaat cacggcgctg aaagcaggct
    45841 ttatgaccac ggtgccattc ctcgcggcgt ttgtcggcgt cctgctctct ggctgggtag
    45901 cggatctgct ggtacgtaag ggcttttcac tgggctttgc gcgtaaaacg ccgattatct
    45961 gcggcttgct gatctccacc tgcattatgg gcgctaacta cactaacgat ccgatgatga
    46021 ttatgtgcct gatggcgctg gcattcttcg gtaacggttt tgcttcgatt acctggtcgc
    46081 tggtttcttc tctggcaccg atgcgcctga ttggtttaac cggcggcgtg tttaacttcg
    46141 ccggtggtct gggcggcatc accgttccgc tggtggtggg gtacctggcg cagggttacg
    46201 gtttcgcacc tgcactggtt tatatctccg ccgtcgcgtt gattggcgcg ctctcttata
    46261 tcctgctggt gggcgatgtg aagcgcgttg gctaatcctt caactgtgga atatgtgcca
    46321 caccactgcg cataatccac tttcttaaag ccggtgcggt caggcatcgg ctttttacta
    46381 tcttgccgaa gagttttaaa cgcagtttgc gggtggcgcg ccagtaagcg gtattgagct
    46441 tctctggctg cttcagtaac accgaacgca gaatgtctgc gctatccagc gcatagctaa
    46501 tcccctccag cgagctggcg ctgataaatc ccgccgcttc accaatcaga aaggcgttgt
    46561 ccttaccgca gacaaaatcc tgccagcgtg agggaaacag caccgtgcat ttttcgcttt
    46621 tcaccgcctt accaaactgg aactgaaagg cgctcatttt ctctttcagc gtcgtgaaac
    46681 gcgtctgacc gtctttcatt ggataagcac cgccaaagat aaaataaccg tctttgctga
    46741 tactccatga ataacagtta gttatcgaat tatcaaagat gcaggagtag aacggcaccg
    46801 gatgtttctc cgcgaaccac tgctggatag cgacatattt acggatttga tgatccgggt
    46861 agagatgtcg ccgcaccatc gagtttgcgc catcggcacc gaccagatag cgggcggtaa
    46921 tatgttgctc ccagccgtct gcacgaaaaa tgacatgcca tttatcatcc tcacgccaga
    46981 tttttcggca caggctatcg tgataaacct caacgctggt ggggatcagc gatttcatcc
    47041 acaagtcgaa agcatggcga ttaatattga tatagcttcg ctggtagtta cgcgtgagcg
    47101 atgcggcgac gtcgacagtt ttgacgctga aaatctgtgg attggcgatc acatcgacag
    47161 gaagcgtcag tccatcgcga ataaaagatc gctgcgcgtc cggtgccagc agaccgccgc
    47221 aaggtttgct gaaaccttca gtaccacact ggtgcttttt atccagcgcg atcacctgca
    47281 ttttgcctgc taattttcgc gccaacgccg atcctgccgg gccgaggcca ataatcgcca
    47341 cgtcgaaatg ttccatcttt tcactcacat tcatcacgat ggcaaaagac tattgtctga
    47401 aagtgaagtc cttgtgaagt atgatggcgg gacccgtttt gtatcaggac cgcgccatga
    47461 aacagataac ctttgctccc cgtaatcacc tgctcaccaa taccaatacc tggacgcccg
    47521 acagccagtg gctggtattt gacgtgcgtc cttctggggc gtcgtttacc ggcgagacca
    47581 ttgagcgtgt gaatatccat accggcgagg tcgaggttat ctatcgcgcg tcacagggcg
    47641 cacacgtcgg cgtggtgacc gttcatccga agtcagagaa atatgttttc atccatggcc
    47701 cggaaaatcc tgatgaaaca tggcattacg atttccatca tcgacgcgga gtcattgctg
    47761 aaggcggcaa ggtgagcaat ctcgatgcaa tggatattac cgcgccgtat acgccaggag
    47821 cgctgcgcgg cggcagccat gtgcatgtct ttagcccgaa cggtgaaagg gtgagcttta
    47881 cctataacga ccatgtaatg catgaactcg atccggcgct ggatttgcga aacgtcggtg
    47941 ttgctgcgcc gtttggcccg gtcaacgtac aaaagcagca tccgcgtgaa tacagcggta
    48001 gccactggtg cgtgctggtg agtaaaacca cgcccacgcc gcagcctggc agcgatgaaa
    48061 tcaatcgtgc ttatgaagaa ggatgggtag gaaatcacgc gctggcgttt attggcgaca
    48121 cactttcgcc aaagggcgag aaagtgccgg agctgtttat cgttgagtta ccgcaagatg
    48181 aagctggctg gaaagcggca ggtgatgcgc cgttaagtgg aacggaaacc accctgcccg
    48241 cgccaccgcg cggcgtcgtg cagcgacgtt taacctttac ccaccatcgg gcttatccgg
    48301 ggttagtcaa cgtcccgcgc cactgggtgc gctgtaatcc gcagggtacg caaatcgcgt
    48361 ttttaatgcg tgatgataac ggcattgtgc aactgtggct tatctcgcca cagggcggcg
    48421 agccgcgcca gttaacccat aacaaaacgg atattcagtc tgcatttaac tggcatccgt
    48481 caggagaatg gttgggcttt gtgctggata atcgaattgc ttgtgcccat gcgcaaagcg
    48541 gcgaggttga gtatttaacc gaaaaccacg ccaatccacc ttctgcggac gccgtggtct
    48601 tctcgccgga tggtcagtgg ctggcgtgga tggaaggcgg ccagctgtgg atcaccgaaa
    48661 ctgatcgcta aatcagttat caggcattct ggccggtgga ataaccgcat tggtggcttg
    48721 cgcgttggct tcgctttttt caacgcgtga gcgcaccgaa ctgtctttgc ggaacacatc
    48781 ccacggcagt aaaagcgtgt ccatcacagc ggtaaacggc atatcgagaa tcgccagcga
    48841 cttagtgccc cagttggttt catcgtcgcc aatcatggtt gcgctggcgc gggtgccggg
    48901 atacgttcct tctttccctc cggtatggga catcacgctg gagcatcctc cgagtaatgt
    48961 cattccgctg catatcatga acgctaacaa cacatttctt atcataataa attcatctgt
    49021 tgatcgtggg tgttggcctg atgagttata gcgatccctt gctgaaaata acatcatcat
    49081 tacgtcgcac tgtggcggct atcgcacttt aacgtttcgt gctgccccct cagtctatgc
    49141 aatagaccat aaactgcaaa aaaagtccgc tgataaggct tgaaaagttc atttccagac
    49201 ccatttttac atcgtagccg atgaggacgc gcctgatggg tgttctggct acctgacctg
    49261 tccattgtgg aaggtcttac attctcgctg atttcaggag ctattgatta tgcgtaactt
    49321 tgatttatcc ccgctttacc gttctgctat tggatttgac cgtttgttta accacttaga
    49381 aaacaaccag agccagagta atggcggcta ccctccgtat aacgttgaac tggtagacga
    49441 aaaccattac cgcattgcta tcgctgtggc tggttttgct gagagcgaac tggaaattac
    49501 cgcccaggat aatctgctgg tggtgaaagg tgctcacgcc gacgaacaaa aagagcgcac
    49561 ctatctgtac cagggcatcg ctgaacgcaa ctttgaacgc aaattccagt tagctgagaa
    49621 cattcatgtt cgtggcgcta acctggtaaa tggtttgctg tatatcgatc tcgaacgcgt
    49681 gattccggaa gcgaaaaaac cgcgccgtat cgaaatcaac taattcctta aggccgcctg
    49741 gcgcggcctg acatctccat gctcgccgtc agggagcata tgcgaatctt cggatttgca
    49801 ggtacttact cgcttcttag aaggagaaat gactatgcgt aacttcgatt tatccccact
    49861 gatgcgtcaa tggatcggtt ttgacaaact ggccaacgca ctgcaaaacg ccggtgaaag
    49921 ccagagcttc ccgccgtaca acattgagaa aagcgacgat aaccactacc gcattaccct
    49981 tgcgctggca ggtttccgtc aggaagattt agagattcaa ctggaaggta cgcgcctgag
    50041 cgtaaaaggc acgccggagc agccaaaaga agagaaaaaa tggctgcatc aagggcttat
    50101 gaatcagcca tttagcctga gctttacgct ggctgaaaat atggaagtct ctggcgcaac
    50161 cttcgtaaac ggtttactgc atattgattt aattcgtaat gagcctgaac ccatcgcagc
    50221 gcagcgtatc gctatcagcg aatgtcccgc gttaaatagc taactagcta ttctctttgc
    50281 cccgccattc tggtggggct ttttttgtct ttcttcttca acaatgcgtt aaatttcgcc
    50341 tgtttaagat atttcggcac gttctttccc gtgataatgt gcgctataag gcaaatctgc
    50401 ttcaccgatt cagccataat ccgtagcaat taaaaatgtt atctcaagag aaatggacga
    50461 tgagtgatat agcattaacg gtcagtattc tggctttggt ggcagtcgtc ggtttgttta
    50521 tcggcaatgt caaatttcgc ggcataggat taggtattgg cggcgtgctg tttggtggga
    50581 tcatcgtcgg ccattttgtt tctcaggcgg ggatgacatt aagtagcgat atgctgcatg
    50641 ttattcagga atttggcctg atcctgttcg tttatactat cgggattcag gtagggccgg
    50701 gcttctttgc ctcattgcgc gtctccggat tacgcctcaa cctgtttgct gttctgatcg
    50761 tcatcatcgg tggtctggtt accgccatcc tgcataaact gtttgatatt ccactgccgg
    50821 tagtgctggg gattttctcc ggtgcggtta ccaatacgcc agcgctgggg gcagggcagc
    50881 agattttgcg cgacctgggt acaccaatgg aaatggtcga tcagatgggg atgagttacg
    50941 cgatggcgta tccattcggc atttgcggga ttttgttcac catgtggatg ttgcgggtta
    51001 ttttccgcgt caatgtcgag acagaagctc agcagcacga gtcttcacgc accaatggcg
    51061 gcgcgctgat caagactatc aatattcgcg ttgagaaccc taacctgcat gatttagcca
    51121 ttaaagatgt accgattctc aacggcgaca aaattatctg ctcgcgtctg aaacgcgaag
    51181 aaaccctaaa agttccttcg ccagatacca ttattcaact gggcgatttg ctgcatctgg
    51241 tgggtcagcc agcggattta cataatgcgc aactggtgat tggtcaggag gtcgatactt
    51301 cgctgtccac gaaaggcact gatttgcgcg tcgagcgtgt ggtggtcacc aatgaaaacg
    51361 tgctcggaaa acgtattcgc gacctgcact ttaaagaacg ctatgacgtt gttatctcgc
    51421 gcctgaaccg tgccggggtc gaactggtcg ccagtggcga tatcagcctg cagttcggcg
    51481 atatcctcaa cctggtgggg cgtccgtccg caattgatgc cgttgccaat gtgctgggga
    51541 atgcgcagca aaaactgcaa caggttcaga tgctgccagt gtttattggc atcgggcttg
    51601 gcgtattgtt aggttctatt cccgtctttg tgccaggatt cccggccgcg ttgaaactgg
    51661 ggctggcggg cggtccgctg attatggcgt tgatcctcgg gcgtatcggc agtatcggca
    51721 agctgtactg gtttatgccg ccaagtgcca acctcgcgct gcgggagctg gggatcgtgc
    51781 tgttcctctc ggtcgttggt ctgaaatctg gtggggattt tgtgaatacc ctggtcaatg
    51841 gcgaagggct aagctggatt ggttatggtg ccctgatcac cgccgttccg ctgattactg
    51901 ttggcattct ggcgcggatg ttagccaaaa tgaattacct gaccatgtgc gggatgctgg
    51961 caggttccat gaccgatcct ccggcgctgg cgtttgctaa taatcttcat ccaaccagtg
    52021 gtgcggcggc gctctcttac gccactgtct atccgttagt gatgtttctg cgaattatca
    52081 ccccccaatt actggcggtg ctcttctgga gtatcggtta actcttttcc gggtggagtc
    52141 gccgtaaatg gtactccacc tggtattcgc tggcatttct gaacatcact gaataattca
    52201 aaaactcccc gctctcgcta tatgacaacg aggtgatccg cagcagtggc gtgtgttccg
    52261 ctaccttcat ttgccgcgcc agcagtctat cggcgagcac cggcgtcagg ctttcataat
    52321 taccgccaat caaaatcccg cactcttgtt caatatattc aaacttcgac ccttccagat
    52381 gttgcagcga aaggttacgg aacagtttta ccggcatata gctgtcttcc agcatcagcg
    52441 gtttcccttc cacaaaacga acgcgacggg agaagtagat ctgctcgttg atttgaatcc
    52501 gtaactggct ggcaatgacc ggaggcgcag gcatgatttc aaaaatcagc acctggctgg
    52561 tgaccgtttt tccctgccgt tttaacacct ccaccagccc ggtcaggctg gcggtttgat
    52621 gcagtacatc tttgcgcacc aggtaagtgc cgctgccgtg gcggcggacc accaatcccc
    52681 acgctaccag caggtcaatg gctttacgga tggtcatccg cgataccgca aattcttccg
    52741 ccaacttttt ttcaccggga agaaggctat ttagcgtgaa atctgcggaa ttaagtcgaa
    52801 ttcttaaccg ctccgcaatg cttttgtaga tcatctctag atctcttttt tgcctgcaag
    52861 cccgggtaat gcgcccacaa gttctgctta atcgattgaa aaaacatctt ttttttaaag
    52921 atgtgttcga tgctgtgtac ctgtgctcac agatgtctac tttttcgcga aaacgtagat
    52981 ctctaccgcc caacgaaaag catgaaagcg atcacgaatc ccattgggtc gtcatgttct
    53041 gccgatcgca tcttcctatc ctcgctccag gcctgccgca taaccaatca ggcttcctac
    53101 ttacagaatt gagaaaagag gatgtggaaa tgctcagtca aattcaacgc tttggcggcg
    53161 cgatgttcac gccagtgctg ctgtttccct tcgccgggat tgtggtgggt cttgccatct
    53221 tgctgcaaaa cccgatgttt gtcggggaat cactgaccga tccgaacagt ttattcgcgc
    53281 aaatcgtaca cattattgaa gagggcggtt ggacggtatt ccgtaatatg ccgctgattt
    53341 ttgctgtcgg tttacccatt ggccttgcta agcaagcgca gggacgtgct tgtctggcgg
    53401 tgatggtgag tttcctgacc tggaactatt tcatcaatgc gatgggaatg acctggggaa
    53461 gctacttcgg cgtcgatttc actcaggacg cggtggcagg tagcggtctg acaatgatgg
    53521 ctgggattaa aaccctcgat accagcatta tcggcgcaat tatcatttcc ggcattgtga
    53581 cggcgctgca taaccgtctg ttcgataaaa aactgccggt gtttctcggc attttccagg
    53641 ggacgtctta tgtggtgatt atcgccttcc tggtgatgat cccctgtgcc tggctgacat
    53701 tgctcggctg gccaaaagta caaatgggga ttgaatctct gcaagcgttc ctgcgttcgg
    53761 cgggtgcgct tggggtgtgg gtttacacct tcctcgaacg tattctgatc ccaaccggtt
    53821 tacaccactt catctacgga ccgtttatct ttggtccggc agctgttgaa ggcggcattc
    53881 agatgtactg ggcgcagcat ctgcaagagt tcagtctgag cgccgagccg ctgaaatcgt
    53941 tgttcccgga aggcggtttt gccctgcacg gtaactcaaa aatctttggt gccgtgggca
    54001 tttctttagc gatgtacttc actgccgcac cggaaaatcg ggtaaaagtg gcgggcttgc
    54061 tgattcccgc aaccttaacc gccatgctgg tgggaattac cgaaccgctg gaatttacct
    54121 tcctgttcat ttcaccgttg ctgtttgcgg tacacgccgt gctggcggcc tcaatgtcga
    54181 ccgtaatgta tctctttggt gtggtgggca acatgggcgg aggtctgatt gaccaggttt
    54241 taccgcaaaa ctggatcccg atgttcagca accacgcgga tatgatgctg acccaaatcg
    54301 ccattgggtt gtgctttacc ctgctgtact tcgtggtttt ccgcacactg attctgcagt
    54361 tcaacatgtg cacgccggga cgtgaagatg cggaagtgaa actctactca aaagccgaat
    54421 acaaagcctc gcgaggccaa accaccgcgg cagagccaaa aaaagagctg gatcaggctg
    54481 ccggtatcct gcaagccctg ggcggggtcg gcaatatctc cagcattaac aattgcgcga
    54541 cgcgtttacg cattgcactg catgacatgt cacaaacgct ggatgacgaa gcctttaaaa
    54601 agctgggagc gcacggcgtc ttccgtagtg gcgatgccat tcaggtgatc attggtctgc
    54661 atgtatccca gctgcgtgaa cagctcgata gcttaattaa ttctcatcaa tcagcagaaa
    54721 atgttgccat tacggaggca gtataatgac caaattctca gtggttgtcg caggcggtgg
    54781 aagcaccttt acgccaggca tcgtgttgat gctcctggcg aatcaggagc gtttcccgct
    54841 tcgtgcactg aaattttatg ataacgatgg cgcgcggcag gaagtgattg ccgaagcctg
    54901 taaagtcatc cttaaagaga aagcgccgga cattgcgttt agttacacca ccgatcctga
    54961 agtggcgttc agcgacgttg attttgtcat ggcgcacatc cgcgtcggca agtacccgat
    55021 gcgcgaactg gatgaaaaaa tcccactgcg ccacggtgtt gttggtcagg aaacttgcgg
    55081 acccggcgga atagcgtacg gcatgcgttc cattggcggc gtcctggaac tggtggatta
    55141 tatggaaaaa tattcaccaa atgcctggat gctcaactac tccaacccgg cagccattgt
    55201 cgcagaagcc acgcgtcgtc tgcgcccgaa tgcgaaaatc ctcaacatct gtgacatgcc
    55261 aatcggtatt gaaagccgga tggcgcaaat tgttgggctg caagatcgca aacagatgcg
    55321 cgtgcgctac tacggcctga accactggtg gtcggcgata tcccgcagtt tcagaaaggg
    55381 ttaatgagtc agcaagtggc ggtggaaaaa ctggtcgtgg acgcctggga acagcgttca
    55441 tatcagcacc tgtggcaggc gattacgttg tcgaaaacgg taccgagcgc ctcggtcgcc
    55501 aaagctattc tggatgagtt gctggaggcc aacaaagcgt actggccaga gttacgttaa
    55561 ttgtgtggac tggcatccgt tgggtgctgg tccgtattct ttagcacggc tgacggcgca
    55621 taaccaaatt tatgtttaaa ggctttgctg aaatgaaagg agtcgaagaa gttgagcgta
    55681 tcagcgactt cgccaaccga gtttccttgt tgctttaata gcgatagcgc cagatccaga
    55741 cgcgcatcga ggtaatattc tttcggcgtc ttgccggtat agcgtaaaaa cagacgacgc
    55801 aaccaggctt cgctacaggg gatcgtggca gccatatcag ctacgctcca gcgttgttgc
    55861 agactggcat gtaaagtggc aattaatttt tcaatttgcc gccgttgtgg atctttttta
    55921 ccatctgcgt acataagaca aatccactga taaataattt tcgtcagaaa agcgactgcc
    55981 agattatttt ttattgcttc tggtgaagtt attaactcag caacttcggt gagttcctga
    56041 ttataaattt cgccattata aataacgctt tgctgaccaa cgggaatatc catcatactg
    56101 gtgggggtaa attccatcca gtactgttcc cagactaaac cttcacagtg ataagagtga
    56161 atatccattg gctttaagaa tataatacag ttgccatgca gtgttatttg ttcgccattc
    56221 tttaaaagaa ttttcccaca tccttgtacg gtatacaccg caacccatga gttggcgata
    56281 agcggtttct tcttgtcccg cggccagatc acccggtaat gctcgtcggc gtaggtatgc
    56341 cagagcgaga tcagagaaat tccgctggag atcacatttt cgttaatcaa taagggaata
    56401 ttgtacggag tttcgttttt tacatctgaa ctttgcaatt ttccattcat tttaatatcc
    56461 ctccggcgaa ttatgctcac aacctgaact taacagaaaa caatccgttc agggatacga
    56521 cgtttgtatt ttaagaatct gactgcctga cccgacgcat tttacctctc cctatattca
    56581 tgcgtccggg acataaacat aaataagggc tatgagatga attcgttaca aatcttgagt
    56641 tttgtcggtt ttacgctgct ggtggcggtg atcacctggt ggaaggtccg caaaacagat
    56701 accggatcgc aacaaggcta ttttcttgcc ggacgttcac taaaagcgcc ggttattgcc
    56761 gcttcgttaa tgctaaccaa cctttccacg gaacaactgg tcggcctttc cgggcaggcc
    56821 tacaaaagcg gcatgtcggt gatgggctgg gaagtgactt cagcggtgac gctgatcttc
    56881 ctcgcgctaa tctttttacc gcgctatctg aagcgcggca ttgccaccat ccccgatttt
    56941 ctggaggaac gttatgataa aacgacgcgt attatcatcg acttctgctt cctcattgcc
    57001 accggcgtct gctttctgcc gattgttctc tactccggcg cgttggcgct caacagcctg
    57061 tttcacgtcg gggaatcgct acagatttct cacggtgcgg ctatctggct actggtaatt
    57121 ttgcttggtc tggcgggaat tttgtatgcg gtgatcggcg gactgcgcgc aatggcagtg
    57181 gcggactcca tcaacggtat tgggctggtt atcggcgggt tgatggtgcc ggtatttggc
    57241 ctgatcgcga tgggcaaggg cagctttatg cagggcattg agcaactcac caccgttcac
    57301 gccgagaaat taaactcaat cggtggcccg accgatccct tgccgattgg cgcggcattt
    57361 accggtttga ttctggtgaa caccttttac tggtgtacaa atcagggcat cgtgcaacgc
    57421 acgctggcgt caaaaagcct ggcggaaggg caaaaggggg cgctgttaac ggcggtgctg
    57481 aaaatgctcg acccgctggt actggtgctg ccagggttga ttgcgtttca tctgtatcag
    57541 gatttaccga aagccgacat ggcctacccg acgctggtca ataacgttct gccagtgcca
    57601 atggtgggtt tcttcggcgc ggtgttattt ggtgcggtga tcagtacctt caacggcttt
    57661 ctgaatagcg ccagtacgtt attcagtatg ggtatttacc gtcgcatcat taaccagaat
    57721 gccgagccgc agcagctggt caccgtcggg cgcaaatttg gtttctttat cgctatcgtt
    57781 tcggtgctgg tcgcgccgtg gatcgccaac gcgccgcagg ggctgtatag ctggatgaaa
    57841 cagctcaacg gcatttacaa cgtgccgctg gttaccatca tcattatggg ctttttcttc
    57901 ccgcgcatcc cggcgctggc ggcaaaagtg gcgatgggga ttggcataat cagctacatc
    57961 accatcaact atctggtgaa gttcgacttc catttcctct atgtgctggc ctgtacgttc
    58021 tgcatcaacg tggtcgtgat gctggtgatc ggttttatca aaccgcgcgc cacgccgttc
    58081 accttcaaag atgcgtttgc ggtggacatg aaaccgtgga gaaacgtcaa gatcgcgtca
    58141 attggcatcc tgttcgcgat gattggcgtc tatgccgggc tggctgaatt cggcggctac
    58201 ggtacgcgct ggttagcgat gatcagttat ttcattgccg ccgtagtgat tgtctacctg
    58261 atttttgaca gctggcggca tcgtcacgac ccagccgtaa cctttactcc cgacgcgaag
    58321 gatagcctat gaaacgcccc aattttctgt tcatcatgac cgatacccag gccaccaata
    58381 tggtcggttg ctatagcggt aaaccgctga atacgcaaaa tattgatagt ctggcggcgg
    58441 aaggtattcg ctttaattcc gcctacacct gttcaccggt ttgtacgcct gcacgcgccg
    58501 gactatttac cggtatctac gctaaccagt ccggcccgtg gaccaacaac gtcgcgctag
    58561 gcaaaaacat ctccactatg gggcgctact ttaaggatgc cggctatcac acctgttaca
    58621 tcggcaaatg gcatctcgac ggtcatgact atttcggcac tggcgagtgt ccgccggagt
    58681 gggacgctga ttactggttc gatggggcga actaccttag cgaactgacg gaaaaagaga
    58741 tcagcctgtg gcgcaatggc ctaaacagcg ttgaggattt acaggcgaac catatcgacg
    58801 aaaccttcac ctgggcgcac cgcatcagca atcgggcggt agattttctg caacagcccg
    58861 cgcgcgccga ggaacccttc ctgatggtgg tttcgtatga tgagccgcat cacccgttca
    58921 cctgtccggt ggagtattta gagaaatacg ctgattttta ctacgatctg ggcgagaaag
    58981 ctcaggatga cctggcgaac aaaccggaac atcaccgctt atgggcgcag gcgatgccat
    59041 cgccagtcgg tgatgacggg ctttatcacc atccgctcta ttttgcctgt aatgactttg
    59101 ttgatgacca aatcggacgg gtcatcaatg ccttaacgcc agagcaacgt gaaaatacgt
    59161 gggtcattta tacttccgat cacggcgaaa tgatgggcgc acataagctg atcagtaaag
    59221 gagcggcgat gtatgacgac atcacccgta ttccgctgat catccgttcg ccgcaagggg
    59281 agcggcgaca ggtcgatacg ccagtcagtc atatcgattt actgccgaca atgatggcgc
    59341 tggcagatat tgaaaaacca gagattctgc cgggggaaaa tatccttgcc gtgaaagagc
    59401 cacgcggtgt aatggtggaa tttaaccgct acgagattga gcatgacagc tttggcggtt
    59461 ttattccggt gcgttgctgg gtgacggatg actttaaact ggtactcaac ctcttcacca
    59521 gtgatgaact ttacgatcgc cgtaatgacc caaatgaaat gcataacctg atcgatgata
    59581 tccgttttgc agacgttcgc agcaaaatgc atgacgcctt attggattac atggacaaaa
    59641 ttcgcgatcc gttccgcagt taccaatgga gcctgcgtcc gtggcgtaaa gatgcactgc
    59701 cgcgctggat gggggcattt cgtccacgcc cacaagatgg ctattcgccg gtggtacgtg
    59761 actatgacac cggcctaccg acgcaaggag tgaaagtgga agagaaaaaa cagaagttct
    59821 gacgataatg cccgctgatg aacacccggc gggcattatt caggctgctt aaaccagtga
    59881 accgccggtc aggaaggaaa aaatcaggaa tatcagcaaa atgctgcaca cgatgccgat
    59941 accaaaaagc aaggtatgga aagccagcgt cccgccgcca aaaaccagcg cgccgcgaat
    60001 gccccagcga ttttccttac gcataccgcc aataaatgcg caaagtagcg cagcaatggc
    60061 cagtgcgata ccgctttgat cgaggatttt atcgacatct acgtcctctt ttttctccac
    60121 ggtggtaatt ttttcgcctt ttattccggc gagaagacct cttttaattt cagccgtttt
    60181 gtccgccacg atgctttcaa acgttggcgg tggtgcggaa aaagggccga atgaaaagtg
    60241 gataatcccc atcattaaag cgatggcacc gaacagcatg cccaatgaac tgattttatt
    60301 ttgagctatg atacccatct gacctccttg tagcggatgg cgaaagcata tcaggaaacg
    60361 ggcggacgaa attcttaaaa aaaaaccgct aaaggtagag cgttagtgaa attgcaaagg
    60421 agcaacaatg aaaatttccc gccttggaga agcgcccgat taccgcttct cgctggctaa
    60481 tgagcgtact tttctggcgt ggatccgtac cgcactagga tttctggcgg cgggggttgg
    60541 gcttgatcaa cttgcgccag atttcgccac gcccgtcatt cgtgaactgc tggcgttgtt
    60601 gttgtgtctg ttttccggcg ggttagcgat gtatggctac ctgcgctggt tacgcaatga
    60661 aaaagcgatg cgtctgaaag aggacctgcc ctacaccaac agcttattaa tcattagctt
    60721 aatattgatg gtcgtggcgg tgatcgttat gggactggtg ttgtatgccg gatagccgca
    60781 aagccagacg cattgccgac ccaggactgc agccggagcg cacatcactg gcgtggtttc
    60841 gtaccatgct gggctacggc gcgttgatgg cgttggctat caaacacaac tggcaccagg
    60901 cgggcatgtt attctggatt tcgattggca ttctcgccat cgtggcgctg atcctctggc
    60961 actacacccg taatcgcaat ttaatggatg tcacgaatag cgatttttcc caatttcacg
    61021 tagtccgtga caaatttttg atctccctcg cggtgttatc tctcgcaata ctgtttgctg
    61081 taacgcatat acatcaactt atcgtattta ttgagagagt cgcatgacag gaagtcaggt
    61141 cattgatgct gaagaggaca ggcataaact ggttgtagag tacaaggatg ctctgcaacc
    61201 tgccgatttt tatcacaatt tcaaacagcg gggcatccgc gccgtacagc tcattcccta
    61261 tcttgaattt gatgaccggg gcgatctgac ggccgcttcg gtgacagcag agctgtgggg
    61321 gaaattttta attgccctgt tcgagtgttg ggtgcgggca gatatcagtc gtatctcgat
    61381 tgagcttttt gacgccacct tacaaaaatg gtgtggcagc gaaaacccgc aaccgcgccg
    61441 tgactgccag gcgtgcgact ggcatcgcct gtgcccgcac gctcgccagg agacgccaga
    61501 cagcgtgctt tgtgcgggct atcaggcgtt ttattcttac tccgcaccgc atatgcgggt
    61561 gatgcgtgat ctgataaagc agcatcgttc tcccatggag ctgatgacga tgctgcggtg
    61621 acgtgcgctt aaacgggctg cccctgatcc gacatccgcg tcgccagcgg cagccagcac
    61681 agcacgatca acaatcccat taaggtcatc aacaacccca ggctgccctg accggtttgc
    61741 ggcaacatcg cagagagcga cgccagcacg ccggaaccaa tgttttgcag accgccgacc
    61801 agcgcgccag ccgtgcccgc caggaagggg aacggctcca tcgcgccgct ggtcgccagc
    61861 ggaaacagca tcccggcacc gaaaaagaac agcgcggcgg gaacgagcag cgtccagaca
    61921 ttcatcacgc caaaccagtc ggggatccac atcagcaagc cagccagcag gcagcagata
    61981 accgactgcc acattaacgt ggagaagcgt ttattgggac gtccggcaaa ccatgcgcca
    62041 aaaaacgctg ccggaatcgg cagaataaac aaaatactga ccgtcatact gctcagccct
    62101 aacaccgcgc ccatcagcac gccggagcag gcttcaaagg cggcaatccc ggccagaccg
    62161 ccaatcagca tcagcaaata acagttaaaa ccgctgttac cgaaaagcgt tttataactg
    62221 gtaagcaggc gcgtgcgcgg tgcatcgacc ggacgcgttt ccggcatcca gcgggccata
    62281 ctgaaggtca caccagcaca aagaaccaac aaaaagagat aacaggcgcg ccagttccac
    62341 atcgtatcca gcagaccgcc gattagcggt gcgagcaacg gactgacgag aatccccatg
    62401 tttaacaggc tgttagcatg gcgcaactgt gtccgttcat ataaatctcg cggcaaagta
    62461 cgcgccatta cgccgccaac gccggtgccc atcccctgca tcgcgctggc ggcaatcaac
    62521 accgtcaaac tggaggtcgt gaccgcgacc agcgttgcca gcataaaaat ggacattccg
    62581 acgaggatca ccggtcggcg gcccacgcgg tcggaaatcg ggccataaaa cagctgtgag
    62641 acaccgtaag tcagcagata agcgcccatt acgctctgca ccgccccttc acggacgttg
    62701 agatcgcgcg ccatatcggc aatagctgga atataaatgg tttgcgccat ctgaccgacg
    62761 gccacgagta ataccaacat caataacaaa ttgacgtttc tttgcctttt cattatcacg
    62821 gatgctttta taaaaataat tagaataagt cactacgcat tcccataaat gcgggagagg
    62881 aatctatcac attgatgaat gttagccaga tatacgccag gaatggggaa ttgtttagcg
    62941 gcagaatatg taaacaaaag cggcaataaa tgttgccggg agggattacg tgcgaactgt
    63001 aaccctctcc ccaaaggggc gagggggaaa ccaccgttcc ctttcagaaa atgagctaaa
    63061 cattgcacgg gacggtcccc tcgccccttt ggggagaggg ctagggtgag gggcacaggc
    63121 agcgcaggtg caaacattct gctggtagaa gaagccccca actccaaccc gcacgctaaa
    63181 tataaaaggg gagcggttac ccgctcccct ttggtgcgac ttgaatctga attacttcag
    63241 gtatttcaga acagcatcaa gcagttgcag tgctgcaaca atgagtttga ggataagaat
    63301 ggcgatatcc accaggctca tacgcgtctc ctgtggttca ggagcactgc cagctgacgt
    63361 acctttccgc tgcctgttgc cagctgttgt gcacgcggta tcttaaggag aaacgttgtg
    63421 cttgttgaca taacacagtg tgctcacgcg gggttaaggc gctgacggca ccacccgttt
    63481 cagccaggac ttagtgcgcc gggtaacgat gcggcccccg cataacacat tgcgtacagt
    63541 gatattatag aagtttactg tataaataaa cagtaatatt tggacaaaac gcaaactgtg
    63601 atcgatactc aactatgccc taaatacgtc aaaattcgtg ccgaaattgc gcgttctgcg
    63661 cggaacacgt atactttcag tgttgacata atacagtgtg ctttgcggtt accagccgca
    63721 ggcggctgat gaaacctcgc tccggcgggg ttttttgtta cctgcaattc agtacaaaac
    63781 gtgatcagcc cctcaatttt cccttgctga aaaattttcc attgtctccc ctgtaaagct
    63841 gtgcttgtat aaatattgtt aaacacaaaa ccaacaaggt ccccaatgac tacttccatg
    63901 ttcaacgcaa aactactacc aactgcgcta tccgccgcag tggtcgtcgt gcgtgtggtg
    63961 gtggtcgtcg gcaatgcgcc gtagggactg gaacaacaca cgattccaaa accccgccgg
    64021 cgcaaaccgg gcggggtttt tcgtttaagc acctcccgga aagtcggccc agaagaaaag
    64081 gactggagca tggcaagttc gggcacaaca tcgacgcgta agcgctttac cggcgcagaa
    64141 tttatcgttc atttcctgga acagcagggc attaagattg tgacgggcat tccgggcggt
    64201 tctatcctgc ctgtttacga tgccttaagc caaagcacgc aaatccgcca tattctggcc
    64261 cgtcatgaac agggcgcggg atttatcgct caggggatgg cgcgcaccga cggtaaaccg
    64321 gcggtctgta tggcctgtag cggaccgggt gcgactaacc tggtgaccgc cattgccgat
    64381 gcgcggctgg actccatccc gctgatttgc atcaccggtc aggttcccgc ctcgatgatc
    64441 ggcaccgacg ccttccagga agtggacacc tacggcatct ctatccccat caccaaacac
    64501 aactatctgg tcagacatat cgaagaactc ccgcaggtca tgagcgatgc cttccgcatt
    64561 gcgcaatcag gccgcccagg cccggtgtgg atagacattc ctaaggatgt gcaaacggca
    64621 gtttttgaga ttgaaacaca gcccgctatg gcagaaaaag ccgccgcccc cgcctttagc
    64681 gaagaaagca ttcgtgacgc agcggcgatg attaacgctg ccaaacgccc ggtgctttat
    64741 ctgggcggcg gtgtgatcaa tgcgcccgca cgggtgcgtg aactggcgga gaaagcgcaa
    64801 ctgcctacca ccatgacttt aatggcgctg ggcatgttgc caaaagcgca tccgttgtcg
    64861 ctgggtatgc tggggatgca cggcgtgcgc agcaccaact atattttgca ggaggcggat
    64921 ttgttgatag tgctcggtgc gcgttttgat gaccgggcga ttggcaaaac cgagcagttc
    64981 tgtccgaatg ccaaaattat tcatgtcgat atcgatcgcg cagagctggg taaaatcaag
    65041 cagccgcatg tggcgattca ggcggatgtt gatgacgtgc tggcgcaatt gatcccgcag
    65101 gtggaagcgc aaccgcgtgc agagtggcac cagttggtag cggatttgca gcgcgaattc
    65161 ccgtgtccaa tcccgaaagc gtgcgatcca ttaagccatt acggcctgat caacgccgtt
    65221 gccgcctgtg tcgatgacaa tgcgattatc accaccgatg tgggtcagca tcagatgtgg
    65281 accgcgcaag cttatccgct caatcgccca cgccagtggc tgacctccgg tgggctgggc
    65341 acgatgggtt ttggcctgcc tgcggcgatt ggcgcggcgc tggcgaaccc ggatcgcaaa
    65401 gtgttgtgtt tctccggcga cggcagcctg atgatgaata ttcaggagat ggcgaccgcc
    65461 agtgaaaatc agctggatgt caaaatcatt ctgatgaaca acgaagcgct ggggctggtg
    65521 catcagcaac agagtctgtt ctacgagcaa ggcgtttttg ccgccaccta tccgggaaaa
    65581 atcaacttta tgcagattgc cgccggattc ggcctcgaaa cctgtgattt gaataacgaa
    65641 gccgatccgc aggctgcatt gcaggaaatc atcaatcgcc ctggcccggc gctgatccat
    65701 gtgcgcattg atgccgaaga aaaagtttac ccgatggtgc cgccaggtgc ggcgaatact
    65761 gaaatggtgg gggaataagc catgcaaaac acaactcatg acaacgtgat tctggagctc
    65821 accgttcgca accatccggg cgtaatgacc cacgtttgtg gcctttttgc ccgccgcgct
    65881 tttaacgttg aaggcattct ttgtctgccg attcaggaca gcgacaaaag ccatatctgg
    65941 ctactggtca atgacgacca gcgtctggag cagatgataa gccaaatcga taagctggaa
    66001 gatgtggtga aagtgcagcg taatcagtcc gatccgacta tgtttaacaa gattgcggtg
    66061 ttttttcagt aaccgctcaa ggcttgaaca acatcgcgct tatcgttaag gtaagcgcgt
    66121 tttttttacc cgccaggaca agaccatgat caccgttgcc cttatagacg atcacctcat
    66181 cgtccgctcc ggctttgcgc agctgctggg gctggaacct gatttgcagg tagttgccga
    66241 gtttggttcg gggcgcgagg cgctggcggg gctgccgggg cgcggtgtgc aggtgtgtat
    66301 ttgcgatatc tccatgcccg atatctccgg tctggaactg ctaagccagc tgccgaaagg
    66361 tatggcgaca ataatgctct ctgttcatga cagtccggcg ctggttgagc aggcgcttaa
    66421 cgcgggggcg cgcggctttc tctccaaacg ctgtagccct gacgaactga ttgcggcggt
    66481 gcatacggtt gccacgggcg gctgttatct gacgccggat attgccatta aactggcatc
    66541 cggtcgtcag gacccgctga ccaaacgtga acgccaggtg gcggaaaaac tggcgcaagg
    66601 aatggcggtg aaagagattg ccgccgaact gggcttgtca ccgaaaacgg tacacgtcca
    66661 tcgcgccaat ctgatggaaa aactgggcgt cagtaacgac gtagaactgg cgcgccgcat
    66721 gtttgatggc tggtgatgaa gacgttgttc tcccgcttaa ttaccgttat tgcctgcttt
    66781 tttatcttct ctgccgcatg gttttgcctg tggagtatca gcctgcacct ggttgagcgc
    66841 cctgatatgg cggtgctgtt atttccgttt ggtctgcggc tagggctaat gctgcaatgc
    66901 ccgcgcggat actggcccgt attgctgggt gcggaatggc tgctgattta ctggctaacg
    66961 caggcggtcg gtttaaccca ttttccgtta ttgatgatcg gtagtttact gacgttactg
    67021 cccgtagcgc tgatctcgcg ctatcgccat cagcgtgact ggcgcacctt gctgttacag
    67081 ggggcggcgt taacggcggc ggcgttgttg cagtcgctgc cctggctttg gcacggcaaa
    67141 gagtcgtgga atgcgctgtt gctgacttta actggcggcc tgacgctggc cccgatatgt
    67201 ctggtgttct ggcactatct cgccaataac acctggctgc cgctcggccc gtcactggtt
    67261 tctcaaccga tcaactggcg cgggcggcat ctggtctggt acttgctgtt gtttgttatt
    67321 agtctctggc tccagttggg attgccggac gaactgtcgc gctttacgcc attctgcctg
    67381 gcgctgccga ttatcgcgct ggcctggcac tacggttggc aaggggcgct gatcgcgacg
    67441 ttgatgaacg ccatcgcgct gatcgccagt caaacctggc gcgatcatcc ggtggattta
    67501 ttgctctcgt tgctggtgca aagtctgaca gggttgttac tgggcgctgg catccagcgg
    67561 ttgcgtgaac ttaaccagtc gctgcaaaag gaactggcgc gcaatcagca tctggctgaa
    67621 cggttgctgg aaaccgaaga gagcgtgcgt cgtgatgtgg cgcgtgagct gcatgatgat
    67681 atcggtcaga ccatcactgc tattcgtact caggcgggca ttgttcagcg gctggcggca
    67741 gataacgcca gcgtgaagca gagcgggcag ctcatcgaac aactatcgct gggcgtttac
    67801 gacgcggtgc gccgtttgtt gggtcggtta cgtccgcgcc agttggatga tctcaccctg
    67861 gagcaggcca tccgctcact gatgcgggaa atggagctgg aagggcgcgg tattgtcagc
    67921 catctcgaat ggcgaatcga tgaatcagca ttaagtgaaa accagcgcgt gacgctgttt
    67981 cgtgtctgcc aggaagggct gaacaacatt gtgaaacatg ctgatgccag cgcggtcacg
    68041 ctgcaaggct ggcagcagga tgaacggttg atgctggtta ttgaagacga tggcagcggt
    68101 ttgccgccgg gttccgggca acaaggtttt ggcctcaccg gaatgcgcga gcgcgtaacg
    68161 gcgctgggtg gcacattaca catttcctgt ctgcacggca cgcgtgtcag cgtttctcta
    68221 cctcaacgct atgtctaagg tttgatgatg ttgccgtttc tgaaagcgcc tgccgatgcg
    68281 ccattaatga ctgataaata tgaaattgat gcccgctatc gctactggcg tcggcatatt
    68341 ctgctgacca tctggctggg ttacgcgctg ttttacttca cgcgtaaaag ttttaacgcc
    68401 gccgtaccag aaatccttgc taacggcgtg ctcagccgta gcgatatcgg cctgttagcg
    68461 accctgtttt acattaccta cggcgtgtcg aagtttgtct ccggcattgt cagcgatcgc
    68521 tcaaatgccc gttattttat ggggataggg cttatcgcca cgggcatcat caacattctg
    68581 tttggcttct cgacgtcgct atgggcgttt gccgtgctct gggtgctgaa cgcctttttc
    68641 cagggctggg gttcaccggt gtgtgcgcgt ctgttaacgg cctggtattc acgtaccgag
    68701 cgcggcggtt ggtgggcatt atggaacacg gcgcataacg tcggcggcgc actcattccc
    68761 attgtgatgg cagcgagcgc gctgcattac ggctggcgtg ccgggatgat gattgctggt
    68821 tgtatggcga tagtcgtggg gatttttctc tgctggcggc tacgcgatcg cccgcaggcg
    68881 ttaggtttac cggcggtcgg tgaatggcgg cacgacgcgc tggaaattgc tcaacaacaa
    68941 gaaggggcag ggttgacgcg taaagagatc ctgaccaaat atgtgttgct gaatccgtat
    69001 atctggctgc tttcgttttg ctatgtgctg gtctatgtgg tccgggcggc gatcaacgac
    69061 tggggcaatt tgtatatgtc cgagacactg ggcgtcgatc tggtcacggc gaatacggca
    69121 gtgacgatgt ttgaactggg cggatttatc ggtgcgctgg tagccggttg gggctcggac
    69181 aaattgttta acggcaaccg tgggccgatg aatttgattt tcgccgccgg aattttgctt
    69241 tcagtcggct cgctgtggct gatgccattt gccagctacg tgatgcaggc aacctgcttc
    69301 ttcaccattg gtttttttgt ctttggcccg cagatgttaa tcggcatggc ggcggcagag
    69361 tgttcccaca aagaggcggc aggggcggcg acggggtttg tcggcttgtt tgcttatctg
    69421 ggggcgtcgc ttgctggttg gccgctggcg aaagtactcg atacctggca ctggagcgga
    69481 ttttttgtgg ttatctctat cgccgccggg atttccgcac tgctgttact gccctttttg
    69541 aacgcccagg caccgcgcga agcgtgatgc atctcacctt ttcacttcat atccggcaaa
    69601 actaagaaat tttccaggtt ttgcctggac gctatctcag gcctgatttg ctgctgattt
    69661 ttacaatgca tgcctcacgc aggtattcat ttcaggagta acccatgctg gctttcttaa
    69721 accaggttcg caagccgacc ctggaccttc cgctcgaagt gcggcgcaaa atgtggttca
    69781 aaccgttcat gcaatcctac ctggtggtct ttatcggcta cctgacgatg tacctgattc
    69841 gcaagaactt taacatcgcg cagaacgaca tgatctcgac ctacgggttg agcatgacgc
    69901 agctggggat gatcggcctg ggtttctcca tcacttatgg cgtgggtaaa acgctggttt
    69961 cctactacgc cgacggcaaa aacaccaaac aattcctgcc gttcatgctg atcctgtctg
    70021 ccatttgtat gctgggcttc agtgccagta tgggcagcgg ctcggttagc ctgttcctga
    70081 tgattgcctt ctacgcctta agcggctttt tccagagtac cggcggttcg tgcagttact
    70141 ccaccatcac caaatggaca ccgcgtcgta agcgcgggac cttcctcggt ttctggaaca
    70201 tttcccataa ccttggcggc gcgggtgcgg ctggtgtggc gctgttcggc gcaaattacc
    70261 tgttcgacgg ccacgtgatc ggcatgttta tcttcccgtc gattatcgcg ttgattgtcg
    70321 gttttatcgg cctgcgttac ggcagcgact ccccggaatc ttatggcctc ggcaaagcgg
    70381 aagagctgtt cggcgaggag atcagcgaag aggacaaaga gacagaatct accgatatga
    70441 ccaagtggca gatctttgtt gagtatgtgc tgaaaaacaa agtgatctgg ctgctgtgct
    70501 tcgccaacat tttcctctat gtggtgcgta ttggtatcga ccagtggtca accgtgtacg
    70561 cgttccagga actgaaactc tctaaagcgg tggcgattca gggctttacg ctgtttgaag
    70621 ctggtgcgct ggtcggtacg ctgctgtggg gctggctctc tgacctggcg aacggtcgcc
    70681 gtggcctggt ggcctgcatc gcgctggcgc tgattatcgc cacgctcggt gtgtatcaac
    70741 atgccagcaa cgaatatatc tatctggctt ctctctttgc gttgggtttc ctggtctttg
    70801 gcccgcaatt gttgattggt gtggctgctg ttggctttgt acctaaaaaa gcgattggcg
    70861 ctgccgatgg tattaaaggc acctttgctt acctgattgg tgacagcttt gccaagttag
    70921 gtctgggaat gattgccgat gggacgccgg tattcggcct taccggttgg gcaggcacct
    70981 tcgccgcact tgatatcgcc gcgattggtt gtatctgcct gatggcgata gtggcggtga
    71041 tggaagaacg caaaatccgc cgcgagaaaa aaattcagca gttgacagtg gcataaacgt
    71101 aactggtgac ttttgcccgg catgacgccg ggctttttta ttattccgtg acttccagcg
    71161 tagtgaaggc aaacttctcg ccatcaaata gcccctgact ggttagtttt agcgcgggga
    71221 tcactggcag agaaagaaac gccatctgaa taaacggctc atcgggtaac ggaccgcatt
    71281 cacgggcggc ggctttcaag gcgtcaattt gttccgccag caactgcgcc gtgtcggtgc
    71341 tcatcaggcc ggcaatgggc aacggcagat gactttggac ctggccgtta cgtaccacgc
    71401 acagcccgcc gccatcctga atcacctgat tgaccgccag cgccatctct tcggcactgc
    71461 gaccgatcac cacaatatta tggctgtcat ggctgaccgt cgcagccagc gcaccttcat
    71521 tcagtccaaa gccgccaagc aaaccacaag ccggagccag ccgttgcccg taacgctcaa
    71581 gtacggcaat aaagctcaca tcatcgcgat caaaaccatt ttcgctgtag acgctggagt
    71641 gggagtgcgt aatcaattcg ttatggatga cgtcaatgac ccgatagcgt tttccgggcg
    71701 taaattgcag ggcaaagtcg ctggcggaaa ctggctggcg ggcaatggtg ttgccatatg
    71761 gcggagcgga ttgtgccagt ctcgccgact cttccgcctg taaggtttgc gcatcaatcg
    71821 gctcgccttt caccagtacc tgctgcaccg tgaccttacg cgcatcgctc aacaggacga
    71881 tatcggcctg cttgccgggt gccagtaagc cgaggtgatt cagaccaaag tggcgcgccg
    71941 tcgaccagct ggcgacgcga tatgccacat gcagcggcac attgtgttgt tcgatcaggc
    72001 ggcgaattaa ggcatcgatg tgtccttcat gggcgatctc ccacgggtta cggtcatcgg
    72061 tacagagcat gcattgcggg ctgttaaatt cgttgatcaa cggtgccagc gcgttgagat
    72121 tgcgggcagc ggacccttcg cggatcatca acgacatgcc gagttgtaat ttccggcgtc
    72181 cttcttccag ctgataactt tcgtggcagt tttcaatacc cgcagtaata taggcgttaa
    72241 gttctttacc acccaaaccc gggcagtgac cgtccagcgt caggtggcga aatgcatcca
    72301 gtttatcgag cagcgcattc tgcccgctaa ttacgccagg gtagtccatc atttctgcaa
    72361 ggccggtaac ctgcggatgg tcccgccagg cgagcatctg ttcaagggta aaactggcac
    72421 cgttaacatc gcagccttcc agggcgggta cgcaagagct gacctgtaag tactggtttt
    72481 gccttgcctg ttcggcacag cgggcaaacc aggcgaatcc ggcttctccc atcacgttga
    72541 cgatttcatg agggtcgcaa ataacggtcg tcaggccgcg cggcagggta gcggtttcaa
    72601 aagtgaccgg cgtcatcatg ctggattcaa tatgcaggtg agcatcaata aaccctggca
    72661 ccgccgttgc gccgcgagca tcaatccgct gcaaagccgg agcatcagcg tattctgcgc
    72721 caacaccggc aatgtaacgt cctttaatca caattgggcc ggaaatttct ccgccattga
    72781 tcaggtcgag aatagagaca ttatcaataa tataatcggc aacagcgtcg ccacgggaaa
    72841 cggctaacaa ttcctggtat tcagcccgg
//
LOCUS       DAZFQD010000007        72382 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.007, whole genome shotgun sequence.
ACCESSION   DAZFQD010000007 DAZFQD010000000
VERSION     DAZFQD010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 72382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 72382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..72382
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.007"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(150..1058)
                     /gene="abgR"
                     /locus_tag="ACHKTW_000493"
     CDS             complement(150..1058)
                     /gene="abgR"
                     /locus_tag="ACHKTW_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO6461575.1"
                     /translation="MAFQVKIHQIRAFVEVARQGSIRGASRMLNMSQPALSKSIQELE
                     EGLAAQLFFRRSKGVTLTDAGESFYQHASLILEELRAAQEEIRQRQGQLAGQINIGMG
                     ASISRSLMPAVISRFHQQHPQVKVRIMEGQLVSMINELRQGELDFTINTYYQGPYDHE
                     FTFEKLLEKQFAIFCRPGHPAIGARSIKQLLDYSWTMPTPHGSYYKQLSELLDDQAQT
                     PQVGVVCETFSACISLVAKSDFLSILPEEMGCDPLHGQGLVMLPVSEILPKAAYYLIQ
                     RRDSRQTPLTASLITQFRRECGYLQS"
     gene            1234..2544
                     /gene="abgA"
                     /locus_tag="ACHKTW_000494"
     CDS             1234..2544
                     /gene="abgA"
                     /locus_tag="ACHKTW_000494"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgA"
                     /protein_id="HFO6461576.1"
                     /translation="MESLNQFVNSLAPKLSHWRRDFHHYAESGWVEFRTATLVAEELQ
                     QLGYSLALGREVVNESSRMGLPDELTLQREFERARQQGALAQWIAVFEGGFTGIVATL
                     DTGRPGPVMAFRVDMDALDLSEEQDVSHRPYRDGFASCNAGMMHACGHDGHTAIGLGL
                     AHTLKQFESGLHGVIKLIFQPAEEGTRGARAMVDAGVVDDVDYFTAVHIGTGVPAGTV
                     VCGSDNFMATTKFDAHFTGTAAHAGAKPEDGHNALLAAAQATLALHAIAPHSEGASRV
                     NVGVMQAGSGRNVVPASALLKVETRGASDVINQYVFDRAQQAIQGAATMYGVGVETRL
                     MGAATASSPSPQWVAWLQSQAAQVAGVNQAIERVEAPAGSEDATLMMARVQQHQGQAS
                     YVVFGTQLAAGHHNEKFDFDEQVLAIAVETLARTALNFPWTRGI"
     gene            2544..3989
                     /gene="abgB"
                     /locus_tag="ACHKTW_000495"
     CDS             2544..3989
                     /gene="abgB"
                     /locus_tag="ACHKTW_000495"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgB"
                     /protein_id="HFO6461577.1"
                     /translation="MQEIYRFIDDAIEADRQRYTDIADQIWDHPETRFEEFWSAEHLA
                     SALESASFTVTRNVGNIPNAFIASFGQGKPVIALLGEYDALAGLSQQAGCAQPTSATP
                     GENGHGCGHNLLGTAAFASAIAVKKWLEQYGQGGTVRFYGCPGEEGGSGKTFMVREGV
                     FDDVDAALTWHPEAFAGMFNTRTLANIQASWCFKGIAAHAANSPHLGRSALDAVTLMT
                     TGTNFLNEHIIEKARVHYAITNSGGISPNVVQAQAEVLYLIRAPEMTDVQHIYDRVAK
                     IAEGAALMTETTVECRFDKACSSYLPNRTLENAMYHALSHFGTPEWNSEELAFAKQIQ
                     ATLTPNDRQNSLNNIAATGGENGKVFALRHRETVLANEVAPYAATDNVLAASTDVGDV
                     SWKLPVAQCFSPCFAVGTPLHTWQLVSQGRTSIAHKGMLLAAKTMAATTVNLFIDSGL
                     LQECQQEHQQVTDTQPYHCPIPKNVTPSPLK"
     gene            4023..5549
                     /gene="abgT"
                     /locus_tag="ACHKTW_000496"
     CDS             4023..5549
                     /gene="abgT"
                     /locus_tag="ACHKTW_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate transporter"
                     /protein_id="HFO6461578.1"
                     /translation="MSMSSIPSSSQSGKLYGWVERIGNKVPHPFLLFIYLIIVLMVTT
                     AILSAFGVSAKNPTDGTPVVVKNLLSVEGLHWFLPNVIKNFSGFAPLGAILALVLGAG
                     LAERVGLLPALMVKMASHVNARYASYMVLFIAFFSHISSDAALVIMPPMGALIFLAVG
                     RHPVAGLLAAIAGVGCGFTANLLIVTTDVLLSGISTEAAAAFNPQMHVSVIDNWYFMA
                     SSVVVLTIVGGLITDKIIEPRLGQWQGNSDEKLQTLTESQRFGLRIAGVVSLLFIAAI
                     ALMVIPENGILRDPINHTVMPSPFIKGIVPLIILFFFVVSLAYGIATRTIRRQADLPH
                     LMIEPMKEMAGFIVMVFPLAQFVAMFNWSNMGKFIAVGLTDVLESSGLSGIPAFVGLA
                     LLSSFLCMFIASGSAIWSILAPIFVPMFMLLGFHPAFAQILFRIADSSVLPLAPVSPF
                     VPLFLGFLQRYKPDAKLGTYYSLVLPYPLIFLVVWLLMLLAWYLVGLPIGPGIYPRLS
                     "
     gene            5560..6075
                     /gene="ogt"
                     /locus_tag="ACHKTW_000497"
     CDS             5560..6075
                     /gene="ogt"
                     /locus_tag="ACHKTW_000497"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFO6461579.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLRDYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            6270..7022
                     /gene="fnr"
                     /locus_tag="ACHKTW_000498"
     CDS             6270..7022
                     /gene="fnr"
                     /locus_tag="ACHKTW_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HFO6461580.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            7174..8124
                     /gene="uspE"
                     /locus_tag="ACHKTW_000499"
     CDS             7174..8124
                     /gene="uspE"
                     /locus_tag="ACHKTW_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HFO6461581.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(8174..8431)
                     /gene="ynaJ"
                     /locus_tag="ACHKTW_000500"
     CDS             complement(8174..8431)
                     /gene="ynaJ"
                     /locus_tag="ACHKTW_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
                     /protein_id="HFO6461582.1"
                     /translation="MIMAKLKSAKGKKFLFGLLAVFIIAASVVTRATIGGVIEQYNIP
                     LSEWTTSMYVIQSSMIFVYSLVFTVLLAIPLGIYFLGGEEQ"
     gene            8675..9706
                     /gene="ynaI"
                     /locus_tag="ACHKTW_000501"
     CDS             8675..9706
                     /gene="ynaI"
                     /locus_tag="ACHKTW_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HFO6461583.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAVGSIINYVIENYKLKFITPGVIDFICTSLIAVILTIKLFLLINQFE
                     KQQIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAG
                     KDILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPN
                     SLFSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLV
                     YFNQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMD
                     NITPQEQGR"
     gene            complement(9757..11370)
                     /gene="mppA"
                     /locus_tag="ACHKTW_000502"
     CDS             complement(9757..11370)
                     /gene="mppA"
                     /locus_tag="ACHKTW_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HFO6461584.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKERV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFTPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSTHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(11707..12606)
                     /gene="pgrR"
                     /locus_tag="ACHKTW_000503"
     CDS             complement(11707..12606)
                     /gene="pgrR"
                     /locus_tag="ACHKTW_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HFO6461585.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMAQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPMLHDIDSAMASLSDLQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLKSHPEIDIQLTIDYGLTDVVSERFDAGVRLGGEMDKDMIAIRI
                     GPDIPMAIVGSPDYFSRRSVPTSVSQLIDHQAINLYLPTSGTANRWRLIRGGREVRVR
                     MEGQLLLNTIDLIIDAAIDGHGLAYLPYDQVERAIKEKKLIRVLDKFTPDLPGYHLYY
                     PHRRHAGSAFSLFIDRLKYKGAV"
     gene            12744..13664
                     /locus_tag="ACHKTW_000504"
     CDS             12744..13664
                     /locus_tag="ACHKTW_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO6461586.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVIYFPPKFDETRQYQAIVLSHPGG
                     GVKEQTAGTYAKKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDISAVIDYLTT
                     LSYVDNTRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSMFRNGWENNVKSID
                     ALPYVEAGSNARTSDISSGEYAVMPLAPMKESDAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITYDAYHMAEVYLTQPMQIVAGSQAGSKWMSDDLYDRASSQDKRYHIV
                     EGANHMDLYDGKAYVAEAISVLAPFFEETL"
     gene            13664..14596
                     /locus_tag="ACHKTW_000505"
     CDS             13664..14596
                     /locus_tag="ACHKTW_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO6461587.1"
                     /translation="MKHIQIRNSDMAWHIAANIQFPPNFDESKQYPAIISVHPFGSCK
                     EQTSGNIYGKALAEKGYLVLAYDASFQGESGGEPRWIEDPTQRVEDISRVIDYAVTLP
                     YVNAERIGVLGVCGGGGYAINAALTEKRIKAVVSITGVNIGRLFREGFSNYDPIGALN
                     AMASQRTKEARGGELQINELLPASLDAAKAHGLTERDVYEATDYYKTPRGQQPGGATK
                     MLFSHAQKTLAWDAFAFTEVLLTQPVMVVVGEKVGAFGAYRDGLEVYGRAMVSQDRQL
                     VSLPDFSHYELYDKPEAVQEALEKVIPFFNTHLG"
     gene            14614..15252
                     /locus_tag="ACHKTW_000506"
     CDS             14614..15252
                     /locus_tag="ACHKTW_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309933.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO6461588.1"
                     /translation="MKNVLILGAGGQIARHVINQLADKQTIKQTLFARQPAKIHKPYP
                     TNSKIIMGDVLNHAALKQAMQGQDVVYANLTGEDLDIQANSVIAAMKACDVKRLIFVL
                     SLGIYDEVPGKFGEWNNAVIGEPLKPFRRAADAIEASGLEYTILRPAWLTDEDIIDYE
                     LTSRNEPFKGTIVSRKSVAALITDIIDKPEKHIGDNIGINQPVTDGDKPLFM"
     gene            15561..16289
                     /gene="mpaA"
                     /locus_tag="ACHKTW_000507"
     CDS             15561..16289
                     /gene="mpaA"
                     /locus_tag="ACHKTW_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HFO6461589.1"
                     /translation="MTVNRPRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     ATNWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLAQAFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(16264..17229)
                     /gene="ycjG"
                     /locus_tag="ACHKTW_000508"
     CDS             complement(16264..17229)
                     /gene="ycjG"
                     /locus_tag="ACHKTW_000508"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016855 - racemase and epimerase activity,
                     acting on amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HFO6461590.1"
                     /translation="MRTVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVSQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPETVITAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALALATEARAQGFSLMLGCM
                     LCTSRAISAALPLVPQVSFADLDGSTWLAVDVEPALQFTTGELHL"
     gene            17348..17854
                     /gene="tpx"
                     /locus_tag="ACHKTW_000509"
     CDS             17348..17854
                     /gene="tpx"
                     /locus_tag="ACHKTW_000509"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HFO6461591.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            complement(17898..19439)
                     /gene="tyrR"
                     /locus_tag="ACHKTW_000510"
     CDS             complement(17898..19439)
                     /gene="tyrR"
                     /locus_tag="ACHKTW_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HFO6461592.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFAQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGMFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(19587..20648)
                     /gene="ycjF"
                     /locus_tag="ACHKTW_000511"
     CDS             complement(19587..20648)
                     /gene="ycjF"
                     /locus_tag="ACHKTW_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjF family protein"
                     /protein_id="HFO6461593.1"
                     /translation="MTEPLKPRIDFDGPLEVDQNPKFRAQQTFDENQAQNFAPATLDE
                     APEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGVQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTARLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(20645..22042)
                     /gene="ycjX"
                     /locus_tag="ACHKTW_000512"
     CDS             complement(20645..22042)
                     /gene="ycjX"
                     /locus_tag="ACHKTW_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HFO6461594.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSVKWRMMCEGLDPLAPADENRLADIAAAWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDAWGESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AATKADHVTNDQHANMVSLLQQLIQDAWQNAAFEGISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            22198..23196
                     /gene="ycjW"
                     /locus_tag="ACHKTW_000513"
     CDS             22198..23196
                     /gene="ycjW"
                     /locus_tag="ACHKTW_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFO6461595.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNLAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLAAHNIAVRD
                     EWIVDGGYTHETALQAARELLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDIPTQELGQQSCELLFRLISGKPSPQNITVATHMTLK"
     gene            complement(23307..24212)
                     /gene="ompG"
                     /locus_tag="ACHKTW_000514"
     CDS             complement(23307..24212)
                     /gene="ompG"
                     /locus_tag="ACHKTW_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
                     /protein_id="HFO6461596.1"
                     /translation="MKKLLPCTALVMCAGMACAQAEERNDWHFNIGAMYEIENVEGYG
                     EDMDGLAEPSVYFNAANGPWRIALAYYQEGPVDYSAGKRGTWFDRPELEVHYQFLEND
                     DFSFGLTGGFRNYGYHYVDEPGKDTANMQRWKIAPDWDVKLTDDLRFNGWLSMYKFAN
                     DLNTTGYADTRVETETGLQYTFNETVALRVNYYLERGFNMDDSRNNGEFSTQEIRAYL
                     PLTLGNHSVTPYTRIGLDRWSNWDWQDDIEREGHDFNRVGLFYGYDFQNGLSVSLEYA
                     FEWQDHDEGDSDKFHYAGVGVNYSF"
     gene            complement(24257..25339)
                     /locus_tag="ACHKTW_000515"
     CDS             complement(24257..25339)
                     /locus_tag="ACHKTW_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO6461597.1"
                     /translation="MAQLSLQHIQKIYDNQVHVVKDFNLEIADKEFIVFVGPSGCGKS
                     TTLRMIAGLEEISGGDLLIDGKRMNDVPAKARNIAMVFQNYALYPHMTVYDNMAFGLK
                     MQKIAKEVIDERVNWAAQILGLREYLKRKPGALSGGQRQRVALGRAIVREAGVFLMDE
                     PLSNLDAKLRVQMRAEISKLHQKLNTTMIYVTHDQTEAMTMATRIVIMKDGIVQQVGA
                     PKTVYNQPANMFVAGFIGSPAMNFIRGTIDGDKFVTETLKLTIPEEKLAVLKTQGSLH
                     KPIVMGIRPEDIHPDAQEENNISAKISVAELTGAEFMLYTTVGGHELVVRAGALNDYH
                     AGENITIHFDMTKCHFFDAETEIAIR"
     gene            complement(25353..26012)
                     /gene="pgmB"
                     /locus_tag="ACHKTW_000516"
     CDS             complement(25353..26012)
                     /gene="pgmB"
                     /locus_tag="ACHKTW_000516"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFO6461598.1"
                     /translation="MKLQGVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNSQERAQLAYRKNLLYVHSLRELTVSAVLPGIRSLL
                     ADLRAQQIPVGLASVSLNAPTILAALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGLTGAQLLLPSTDSLTWSRLSAFWQN
                     V"
     gene            complement(26009..28276)
                     /gene="ycjT"
                     /locus_tag="ACHKTW_000517"
     CDS             complement(26009..28276)
                     /gene="ycjT"
                     /locus_tag="ACHKTW_000517"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HFO6461599.1"
                     /translation="MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLAGLYHRAGKGEINELVNLPDILGMEIAINGEVFSLSREAWQRELDFASGELR
                     RSVVWRTSNGTGYTIASRRFVSADQLPLIVLEITITPLDADASVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCMVSGDVQQCYTAKERRLQQHTS
                     AQLHAGETVTLQKLVWIDWRDDRQAVLDEWGSASLRQLEMCAQQSYDQLLAVSTENWR
                     QWWQKRRITVNGGEAHDQQALDYALYHLRIMTPAHDERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTIARSLLRYRWHNLPGAQEKARRNGWQGALFPWESARSGEEETP
                     LFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWQTTGDESFIAHEGMALLLETAK
                     FWISRAVRVNDRLEIHDVIGPDEYTEHVNNNAFTSYMAYYNVQQALSIARQFGCSDDA
                     FIHRAEMFLKELRLPEIQPDGVLPQDDSFMAKPAINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQFSAASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LTQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRDGELHLNPA
                     LPEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLNGQLISVAEESVFCLGDF
                     ILPFNGTATTHQEDE"
     gene            complement(28273..29328)
                     /gene="ycjS"
                     /locus_tag="ACHKTW_000518"
     CDS             complement(28273..29328)
                     /gene="ycjS"
                     /locus_tag="ACHKTW_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFO6461600.1"
                     /translation="MKSAMTSSPLRVAIIGAGQVADKVHASYYCTRNDLELVAVCDSR
                     LSQAQALAEKYGNASVWDDPQAMLLAVKPDVVSVCSPNRFHYEHTLMALEAGCHVMCE
                     KPPAMTPEQAREMCDTARKLGKVLAYDFHHRFALDTQQLREQVTNGVLGEIYVTTARA
                     LRRCGVPGWGVFTNKELQGGGPLIDIGIHMLDAAMYVLGFPAVKSVNAHSFQKIGTQK
                     SCGQFGEWDPATYSVEDSLFGTIEFHNGGILWLETSFALNIREQSIMNVSFCGDKAGA
                     TLFPAHIYTDNNGELMTLMQRELADDNRHLRSMEAFINHVQGKPVMIADAEQGYIIQQ
                     LVAALYQSAETGTRVEL"
     gene            complement(29338..30126)
                     /gene="ycjR"
                     /locus_tag="ACHKTW_000519"
     CDS             complement(29338..30126)
                     /gene="ycjR"
                     /locus_tag="ACHKTW_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFO6461601.1"
                     /translation="MKIGTQNQAFFPENILEKFRYIKEMGFDGFEIDGKLLVNNLEEV
                     KAAIKETGLPVTTACGGYDGWIGDFIEERRLNGLKQIERILEALAEVGGKGIVVPAAW
                     GMFTFRLPPMTSPRSLDGDRKMVSDSLRTLEQVAARTGTVVYLEPLNRYQDHMINTLA
                     DARRYIVENDLKHVQIIGDFYHMNIEEDNLAQALHDNRDLLGHVHIADNHRYQPGSGT
                     LDFHALFEQLRADNYQGYVVYEGRIRAEDPAQAYRDSLTWLRTC"
     gene            complement(30144..31196)
                     /gene="ycjQ"
                     /locus_tag="ACHKTW_000520"
     CDS             complement(30144..31196)
                     /gene="ycjQ"
                     /locus_tag="ACHKTW_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HFO6461602.1"
                     /translation="MKKLVATAPRVAALVEYEDRAILANEVKIRVRFGAPKHGTEVVD
                     FCAASPFIDEDFNGEWQMFTPRPADAPRGIEFGKFQLGNMVVGDIIECGSDVTDYAVG
                     DSVCGYGPLSETVIINAVNNYKLRKMPQGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAYRCDIARRHGADFCLNPIGTDVGKEI
                     KTLTGKQGADVIIETSGYADALQSALRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWELLMNGYLNCEDLIDPVVTFANSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            complement(31227..32069)
                     /gene="ycjP"
                     /locus_tag="ACHKTW_000521"
     CDS             complement(31227..32069)
                     /gene="ycjP"
                     /locus_tag="ACHKTW_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFO6461603.1"
                     /translation="MATNKRTLSRIGFCCGLALFLIITLFPFFVMLMTSFKGAKEAIS
                     LHPTLLPQQWTLEHYVDIFNPMIFPFVDYFRNSLVVSVVSSVVAVFLGILGAYALSRL
                     RFKGRMTINASFYTVYMFSGILLVVPLFKIITALGIYDTEMALIITMVTQTLPTAVFM
                     LKSYFDTIPDEIEEAAMMDGLNRLQIIFRITVPLAMSGLISVFVYCFMVAWNDYLFAS
                     IFLSSASNFTLPVGLNALFSTPDYIWGRMMAASLVTALPVVIMYALSERFIKSGLTAG
                     GVKG"
     gene            complement(32056..32937)
                     /gene="ycjO"
                     /locus_tag="ACHKTW_000522"
     CDS             complement(32056..32937)
                     /gene="ycjO"
                     /locus_tag="ACHKTW_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFO6461604.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            complement(32958..34250)
                     /gene="ycjN"
                     /locus_tag="ACHKTW_000523"
     CDS             complement(32958..34250)
                     /gene="ycjN"
                     /locus_tag="ACHKTW_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFO6461605.1"
                     /translation="MIKSKIVLLSALVSCALISGCKEENKTNVSIEFMHSSVEQERQA
                     VISKLIARFEKENPGITVKQVPVEEDAYNTKVITLSRSGSLPEVIETSHDYAKVMDKE
                     QLIDRKAVATVISNVGEGAFYDGVLRIVRTEDGSAWTGVPVSAWIGGIWYRKDVLAKA
                     GLEEPKNWQQLLDVAQKLNDPANKKYGIALPTAESVLTEQSFSQFALSNQANVFNAEG
                     KITLDTPEMMQALTYYRDLAANTMPGSNDIMEVKDAFMNGTAPMAIYSTYILPAVIKE
                     GDPKNVGFVVPTEKNSAVYGMLTSLTITAGQKTEETEAAEKFVTFMEQADNIADWVMM
                     SPGAALPVNKAVVTTATWKDNDVIKALGELPNQLIGELPNIQVFGAVGDKNFTRMGDV
                     TGSGVVSSMVHNVTVGKADLPGTLQASQKKLDELIEQH"
     gene            complement(34264..35943)
                     /gene="ycjM"
                     /locus_tag="ACHKTW_000524"
     CDS             complement(34264..35943)
                     /gene="ycjM"
                     /locus_tag="ACHKTW_000524"
                     /EC_number="2.4.1.352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosylglycerate phosphorylase"
                     /protein_id="HFO6461606.1"
                     /translation="MKQKITDYLDEIYGGTFTATHLQKLVTRLESAKRLITQRRKKHW
                     DESDVVLITYADQFHSNDLKPLPTFNQFYHQWLQSIFSHVHLLPFYPWSSDDGFSVID
                     YHQVASEAGEWQDIQQLGECSHLMFDFVCNHMSAKSEWFKNYLQQHPGFEDFFIAVDP
                     QTDLSAVTRPRALPLLTPFQMRDHSTRHLWTTFSDDQIDLNYRSPEVLLAMVDVLLCY
                     LAKGAEYVRLDAVGFMWKEPGKSCIHLEKTHLIIKLLRSIIDNVAPGTVIITETNVPH
                     KDNIAYFGAGDDEAHMVYQFSLPPLVLHAVQKQNVEALCAWAQNLTLPSSNTTWFNFL
                     ASHDGIGLNPLRGLLPESEILELVEALQQEGALVNWKNNPDGTRSPYEINVTYMDALS
                     RRESSDEERCARFILAHAILLSFPGVPAIYIQSILGSRNDYAGVEKLGYNRAINRKKY
                     HSKEITRELNDEATLRHAVYHELSRLITLRRSHNEFHPDNNFTIDTINSSVMRIQRSN
                     ADGNCLTGLFNVSKNIQHVNITNLHGRDLISEVDILGNEITLRLWQVMWIK"
     gene            complement(36156..36470)
                     /gene="pspE"
                     /locus_tag="ACHKTW_000525"
     CDS             complement(36156..36470)
                     /gene="pspE"
                     /locus_tag="ACHKTW_000525"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HFO6461607.1"
                     /translation="MFKKGLLALALVFSLPVFAAEHWIDVRVPEQYQQEHVQGAINIP
                     LKEVKERIATAVPDKNDTVKVYCSAGRQSGQAKEILSEMGYTHVENAGGLKDIAMPKV
                     KG"
     gene            complement(36545..36766)
                     /gene="pspD"
                     /locus_tag="ACHKTW_000526"
     CDS             complement(36545..36766)
                     /gene="pspD"
                     /locus_tag="ACHKTW_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HFO6461608.1"
                     /translation="MNTRWQQAGQKVKPGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(36775..37134)
                     /gene="pspC"
                     /locus_tag="ACHKTW_000527"
     CDS             complement(36775..37134)
                     /gene="pspC"
                     /locus_tag="ACHKTW_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HFO6461609.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPAKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(37134..37358)
                     /gene="pspB"
                     /locus_tag="ACHKTW_000528"
     CDS             complement(37134..37358)
                     /gene="pspB"
                     /locus_tag="ACHKTW_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009271 - phage shock [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HFO6461610.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLADEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(37412..38080)
                     /gene="pspA"
                     /locus_tag="ACHKTW_000529"
     CDS             complement(37412..38080)
                     /gene="pspA"
                     /locus_tag="ACHKTW_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HFO6461611.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            38247..39224
                     /gene="pspF"
                     /locus_tag="ACHKTW_000530"
     CDS             38247..39224
                     /gene="pspF"
                     /locus_tag="ACHKTW_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HFO6461612.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPPEDAIAVSETTSLPTLP
                     LDLREFQMQQEKELLQLSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(39344..40609)
                     /gene="puuE"
                     /locus_tag="ACHKTW_000531"
     CDS             complement(39344..40609)
                     /gene="puuE"
                     /locus_tag="ACHKTW_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
                     /protein_id="HFO6461613.1"
                     /translation="MSNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYID
                     FAAGIAVLNTGHRHPDLVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINALAPVSGQA
                     KTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
                     FPGSVYHVPYPSDLHGISTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGEGGFNVAPK
                     ELVAAIRRLCDEHGIVMIADEVQSGFARTGKLFAMDHYADKPDLMTMAKSLAGGMPLS
                     GVVGNANIMDAPAPGGLGGTYAGNPLAVAAAHAVLNIIDKESLCERANQLGQRLKNTL
                     IDAKESVPAIAAVRGLGSMIAAEFNDPQTGKPSAAIAQKIQQRALAQGLLLLTCGAYG
                     NVIRFLYPLTIPDVQFDAAMKILQDALSD"
     gene            complement(40647..41927)
                     /gene="puuB"
                     /locus_tag="ACHKTW_000532"
     CDS             complement(40647..41927)
                     /gene="puuB"
                     /locus_tag="ACHKTW_000532"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HFO6461614.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESISCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWERYGNKQLELLDANAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTAKGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARDPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSRMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPHYPFPGGRTLRVPFTAMGAAYYSLRDRLGV"
     gene            complement(41929..43416)
                     /gene="puuC"
                     /locus_tag="ACHKTW_000533"
     CDS             complement(41929..43416)
                     /gene="puuC"
                     /locus_tag="ACHKTW_000533"
                     /EC_number="1.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
                     /protein_id="HFO6461615.1"
                     /translation="MNFHHLAYWQDKALSLAIENRLFINGEYTAAAENETFETVDPVT
                     QAPLAKIARGKSVDIDRAVSAARGVFERGDWSLSSPAKRKAVLNKLADLMEAHAEELA
                     LLETLDTGKPIRHSLRDDIPGAARAIRWYAEAIDKVYGEVATTSSHELAMIVREPVGV
                     IAAIVPWNFPLLLTCWKLGPALAAGNSVILKPSEKSPLSAIRLAGLAKEAGLPDGVLN
                     VVTGFGHEAGQALSRHNDIDAIAFTGSTRTGKQLLKDAGDSNMKRVWLEAGGKSANLV
                     FADCPDLQKAASATAAGIFYNQGQVCIAGTRLLLEESIADEFLALLKQQAQNWQPGHP
                     LDPATTMGTLIDCAHADSVHSFIREGESKGQLLLDGRNAELAAAIGPTIFVDVDPNAS
                     LSREEIFGPVLVVTRFTSEDQALQLANDSQYGLGAAVWTRDLSRAHRMSRRLKAGSVF
                     VNNYNDGDMTVPFGGYKQSGNGRDKSLHALEKFTELKTIWISLEA"
     gene            complement(43691..44248)
                     /gene="puuR"
                     /locus_tag="ACHKTW_000534"
     CDS             complement(43691..44248)
                     /gene="puuR"
                     /locus_tag="ACHKTW_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HFO6461616.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(44275..45039)
                     /gene="puuD"
                     /locus_tag="ACHKTW_000535"
     CDS             complement(44275..45039)
                     /gene="puuD"
                     /locus_tag="ACHKTW_000535"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HFO6461617.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPGSPSNVQPHLYGENGDEPDADPGRDLLSMALINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITACQHHIAEKQRL"
     gene            45251..46669
                     /gene="puuA"
                     /locus_tag="ACHKTW_000536"
     CDS             45251..46669
                     /gene="puuA"
                     /locus_tag="ACHKTW_000536"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415813.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HFO6461618.1"
                     /translation="METNIVEVENFVQQSEERRGSAFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDAEPRNVLNRLWQQLRQRGLFPVVAVEL
                     EFYLLDRQRDAEGYLQPPCAPGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIP
                     ADGAVAEASPGQFEINLYHTDNVLEACDDALALKRLVRLMAEKHKMHATFMAKPYEEH
                     AGSGMHIHISMQNNRGENVLSDAEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRR
                     FQPGMYVPTQASWGHNNRTVALRIPCGDRHNHRVEYRVAGADANPYLVMAAIFAGILH
                     GLDNELPLQEEVEGNGLEQEGLPFPIRQSDALGEFIENDHLRRYLGERFCHVYHACKN
                     DELLQFERLITETEIEWMLKNA"
     gene            46818..46982
                     /locus_tag="ACHKTW_000537"
     CDS             46818..46982
                     /locus_tag="ACHKTW_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6461619.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRLTRPQTL
                     LRRGGSNYGY"
     gene            46972..48357
                     /gene="puuP"
                     /locus_tag="ACHKTW_000538"
     CDS             46972..48357
                     /gene="puuP"
                     /locus_tag="ACHKTW_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HFO6461620.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVAMSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKEHFHYLLMPLVGALTVGVLWINLEATSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YEGDRTPVSET"
     gene            48491..48736
                     /gene="ymjA"
                     /locus_tag="ACHKTW_000539"
     CDS             48491..48736
                     /gene="ymjA"
                     /locus_tag="ACHKTW_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2543 family protein"
                     /protein_id="HFO6461621.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVADAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            49049..50692
                     /gene="sapA"
                     /locus_tag="ACHKTW_000540"
     CDS             49049..50692
                     /gene="sapA"
                     /locus_tag="ACHKTW_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein SapA"
                     /protein_id="HFO6461622.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            50689..51654
                     /gene="sapB"
                     /locus_tag="ACHKTW_000541"
     CDS             50689..51654
                     /gene="sapB"
                     /locus_tag="ACHKTW_000541"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine export ABC transporter permease SapB"
                     /protein_id="HFO6461623.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKEVFPATMELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            51641..52531
                     /gene="sapC"
                     /locus_tag="ACHKTW_000542"
     CDS             51641..52531
                     /gene="sapC"
                     /locus_tag="ACHKTW_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HFO6461624.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            52531..53523
                     /gene="sapD"
                     /locus_tag="ACHKTW_000543"
     CDS             52531..53523
                     /gene="sapD"
                     /locus_tag="ACHKTW_000543"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HFO6461625.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            53525..54331
                     /gene="sapF"
                     /locus_tag="ACHKTW_000544"
     CDS             53525..54331
                     /gene="sapF"
                     /locus_tag="ACHKTW_000544"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HFO6461626.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            54399..54752
                     /gene="ycjD"
                     /locus_tag="ACHKTW_000545"
     CDS             54399..54752
                     /gene="ycjD"
                     /locus_tag="ACHKTW_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HFO6461627.1"
                     /translation="MMDKIKSNARDLRSNLTLQERKLWRYLRSRRFGDFKFRRQHPVG
                     SYILDFACCSARVVVELDGGQHDLAVAYDTRRTSWLESQGWTVLRFWHNEIDCNEEAV
                     LEIILQELNRRSPSP"
     gene            55120..55908
                     /gene="fabI"
                     /locus_tag="ACHKTW_000546"
     CDS             55120..55908
                     /gene="fabI"
                     /locus_tag="ACHKTW_000546"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFO6461628.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            56053..57180
                     /gene="yciW"
                     /locus_tag="ACHKTW_000547"
     CDS             56053..57180
                     /gene="yciW"
                     /locus_tag="ACHKTW_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HFO6461629.1"
                     /translation="MEQRHITGKSHWYHETQSSTAEYDVLPLVPEAAKVSDPFLLDVI
                     LDEETLAPFLSWLVPARVLAVELFPDQLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSSRESNHRLAQITQYARQLASSPSIIDNRSRQHLNDVGLTAGDC
                     VIINQIIGFIGFQARTIATFQAYLGHPVRWLPGLEIQNYADASLFADESIRWRSSYEV
                     EKLPEEYTKSSTAELCQLAETLSLHPISLSLLERLLNSTRVNTQPDNKLAALLCARIN
                     GSPACFAACMDSSNEYKKISPLLRKGENEINQWADRHSVEHATVQAIQWLTRAPDRFS
                     AAQFSPLLEHEKSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            57248..59182
                     /gene="rnb"
                     /locus_tag="ACHKTW_000548"
     CDS             57248..59182
                     /gene="rnb"
                     /locus_tag="ACHKTW_000548"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008859 - exoribonuclease II activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HFO6461630.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLAIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTALDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWKPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISTEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTDTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAMRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
     gene            complement(59260..59364)
                     /locus_tag="ACHKTW_000549"
     CDS             complement(59260..59364)
                     /locus_tag="ACHKTW_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6461631.1"
                     /translation="MTYMKLTAAKRIVFFIYLFVIQFWKNASFQTKVS"
     gene            59418..61403
                     /gene="pdeR"
                     /locus_tag="ACHKTW_000550"
     CDS             59418..61403
                     /gene="pdeR"
                     /locus_tag="ACHKTW_000550"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HFO6461632.1"
                     /translation="MKTVRESTTLYNFLGSHNPYWRLTESSDVLRFSTTETTEPDRTL
                     QLSAEQAARIREMTVITSSLMMSLTVDESDLSVHLVGRKINKREWAGNASAWHDTPAV
                     ARDLSHGLSFAEQVVSEAHSAIVILDSRGNIQRFNRLCEDYTGLKEHDVIGQSVFKLF
                     MSRREAAASRRNNRVFFRSGNAYEVELWIPTCKGQRLFLFRNKFVHSGSGKNEIFLIC
                     SGTDITEERRAQERLRILANTDSFTGLPNRNAMQDLIDHAINHADNNKVGVVYLDLDN
                     FKKVNDAYGHLFGDQLLRDVSLAILSCLEHDQVLARPGGDEFLVLASNTSQSALEAMA
                     SRILTRLRLPFRIGLIEVYTSCSVGIALSPEHGSDSTAIIRHADTAMYTAKEGGRGQF
                     CVFTPEMNQRVFEYLWLDTNLRKALENDQLVIHYQPKITWRGEVRSLEALVRWQSPER
                     GLIPPLDFISYAEESGLIVPLGRWVILDVVRQVAKWRDKGINLRVAVNISARQLADQT
                     IFTALKQVLQELNFEYCPIDVELTESCLIENDELALSVIQQFSQLGAQVHLDDFGTGY
                     SSLSQLARFPIDAIKLDQVFVRDIHKQPVSQSLVRAIVAVAQALNLQVIAEGVESAKE
                     DAFLTKNGINERQGFLFAKPMPAVAFERWYKRYLKRA"
     gene            complement(61385..61513)
                     /gene="ymiD"
                     /locus_tag="ACHKTW_000551"
     CDS             complement(61385..61513)
                     /gene="ymiD"
                     /locus_tag="ACHKTW_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmiD"
                     /protein_id="HFO6461633.1"
                     /translation="MQKLPLKEKCLTATANYHPGIRYIMTGYSAKYIYSSTYARFR"
     gene            61550..61723
                     /gene="yciZ"
                     /locus_tag="ACHKTW_000552"
     CDS             61550..61723
                     /gene="yciZ"
                     /locus_tag="ACHKTW_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciZ"
                     /protein_id="HFO6461634.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKAPWEI"
     gene            61813..62562
                     /gene="yciT"
                     /locus_tag="ACHKTW_000553"
     CDS             61813..62562
                     /gene="yciT"
                     /locus_tag="ACHKTW_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YciT"
                     /protein_id="HFO6461635.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            62831..63049
                     /gene="osmB"
                     /locus_tag="ACHKTW_000554"
     CDS             62831..63049
                     /gene="osmB"
                     /locus_tag="ACHKTW_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HFO6461636.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(63175..63501)
                     /gene="yciH"
                     /locus_tag="ACHKTW_000555"
     CDS             complement(63175..63501)
                     /gene="yciH"
                     /locus_tag="ACHKTW_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HFO6461637.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGVVRIQRQTSGRKG
                     KGVCLITGVDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(63501..64238)
                     /gene="pyrF"
                     /locus_tag="ACHKTW_000556"
     CDS             complement(63501..64238)
                     /gene="pyrF"
                     /locus_tag="ACHKTW_000556"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFO6461638.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNRDDALSFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLADLGVTLSPADYAERLAALTQK
                     CGLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSDAGDQRRIMTPEQALAAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            complement(64431..65600)
                     /gene="lapB"
                     /locus_tag="ACHKTW_000557"
     CDS             complement(64431..65600)
                     /gene="lapB"
                     /locus_tag="ACHKTW_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0008653 - lipopolysaccharide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HFO6461639.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYRCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            complement(65607..65915)
                     /gene="lapA"
                     /locus_tag="ACHKTW_000558"
     CDS             complement(65607..65915)
                     /gene="lapA"
                     /locus_tag="ACHKTW_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HFO6461640.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVVPHSSAAKE
                     "
     gene            complement(66064..66828)
                     /gene="pgpB"
                     /locus_tag="ACHKTW_000559"
     CDS             complement(66064..66828)
                     /gene="pgpB"
                     /locus_tag="ACHKTW_000559"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HFO6461641.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFAILAAAILVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            66998..67588
                     /gene="ribA"
                     /locus_tag="ACHKTW_000560"
     CDS             66998..67588
                     /gene="ribA"
                     /locus_tag="ACHKTW_000560"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HFO6461642.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            complement(67652..70327)
                     /gene="acnA"
                     /locus_tag="ACHKTW_000561"
     CDS             complement(67652..70327)
                     /gene="acnA"
                     /locus_tag="ACHKTW_000561"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HFO6461643.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     NGLGVLGWGVGGIEAEAAMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLR
                     KHGVVGKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSE
                     DQVELVEKYAKAQGMWRNPGDEPIFTSTLELDMNDVEASLAGPKRPQDRVALPDVPKA
                     FAASNELEVNATHKDRQPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLL
                     AKKAVTLGLKRQPWVKASLAPGSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCIGNS
                     GPLPDPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNI
                     NLASEPIGHDRKGDPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEIN
                     VTRSDTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGS
                     IKPDSPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTR
                     HLPDSDVVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESF
                     ERIHRSNLIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADG
                     SQEVVPCRCRIDTATELTYYQNDGILHYVIRNMLK"
     gene            complement(70491..70586)
                     /gene="ymiC"
                     /locus_tag="ACHKTW_000562"
     CDS             complement(70491..70586)
                     /gene="ymiC"
                     /locus_tag="ACHKTW_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YmiC"
                     /protein_id="HFO6461644.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            complement(70700..70867)
                     /gene="yciX"
                     /locus_tag="ACHKTW_000563"
     CDS             complement(70700..70867)
                     /gene="yciX"
                     /locus_tag="ACHKTW_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciX"
                     /protein_id="HFO6461645.1"
                     /translation="MVGQEQLESSPLCQHSDNETETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            complement(70870..71010)
                     /gene="ymiA"
                     /locus_tag="ACHKTW_000564"
     CDS             complement(70870..71010)
                     /gene="ymiA"
                     /locus_tag="ACHKTW_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588449.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HFO6461646.1"
                     /translation="MRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVVVALLIWKV
                     WG"
     gene            complement(71329..72303)
                     /gene="cysB"
                     /locus_tag="ACHKTW_000565"
     CDS             complement(71329..72303)
                     /gene="cysB"
                     /locus_tag="ACHKTW_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HFO6461647.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGSPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
BASE COUNT        17588 a        18821 c        17887 g        18086 t
ORIGIN      
        1 actgaagaaa attggcaact aaaggttaaa accgttataa cacagtcacc ggcgcagagg
       61 agacaatgcc ggacttaaga cgcggatgca ctgctgtgtg tactgtagag tctggcggat
      121 gtcgacagac tctatttttt tatgcagttt taactttgca gatagccgca ttctcgccgg
      181 aattgcgtga ttaatgacgc ggtcagtggt gtctggcgac tatcacgccg ctgaatcaaa
      241 taataggccg ctttcggtaa aatttcgcta accggcaaca tcaccagccc ctgtccgtgc
      301 aagggatcgc agcccatttc ttcaggcagt atgctgagaa aatcgctttt tgccaccaga
      361 ctgatacagg ctgagaacgt ctcgcagact acaccgacct gtggcgtttg cgcctgatcg
      421 tcaagcaatt cactcaactg tttgtagtaa ctgccgtgtg gcgtcggcat tgtccagctg
      481 taatccagta actgtttgat cgaacgggca ccaatggcgg ggtgtcccgg gcggcaaaag
      541 atcgcgaatt gcttttccag taatttctca aaagtaaatt cgtggtcgta cggtccctga
      601 taataggtat tgatggtgaa atccaattct ccctgacgca attcattaat catcgacacc
      661 agttgccctt ccataatgcg tacttttacc tgcggatgct gctgatgaaa acgagatatg
      721 acagctggca tcagactgcg ggaaatactg gcccccatgc cgatattaat ctgccctgcc
      781 agttgccctt gtcgttggcg aatttcctct tgcgctgcgc gcagctcttc aagaataaga
      841 ctggcgtgct gataaaaact ttcaccggca tcagttaacg tcacgccttt actacggcga
      901 aaaaagagtt gcgccgctaa tccttcttcc agctcctgaa tagatttact cagtgccggt
      961 tgcgacatat tcaacattcg gctcgctccg cgaatgctgc cctgacgagc cacttcaaca
     1021 aaagcacgaa tttgatgaat ttttacctga aaagccatga cgccaccgat aaccgttatt
     1081 tatcagacca aagaaactgg catctacttt aatgcagatg attgtgtcag ggtaatttat
     1141 gaacggttaa aactgtgaaa aatcagttag tgataagtaa aaactatcgc tacgtgaacc
     1201 gggtcacact ttttactgat gacgggaaag gttatggagt ctttgaatca atttgttaat
     1261 tcgcttgccc caaaattatc gcactggcga cgtgattttc atcactatgc agagtctggc
     1321 tgggtggaat tccgcactgc cacccttgtt gcggaagaat tgcagcagct cggctattca
     1381 ctggcgctgg gccgcgaagt agttaatgaa agtagccgga tgggactacc tgatgaactc
     1441 actctacaac gcgaattcga gcgcgctcgt caacagggtg cgctagcaca atggattgcg
     1501 gtttttgaag gtggtttcac tggtatcgtc gccaccctgg ataccggtcg ccccggtccg
     1561 gtgatggctt tccgtgtcga tatggacgcg ctggatctca gtgaagagca ggatgtcagc
     1621 catcgcccct accgcgacgg ttttgcgtca tgtaacgccg gaatgatgca tgcctgtggt
     1681 catgatggac ataccgccat tgggcttggg ctggcgcata cccttaaaca attcgagtcc
     1741 ggactacatg gcgtcatcaa actgattttt cagcctgcag aggaaggtac gcgtggcgcg
     1801 cgggcgatgg tcgatgcagg tgtcgtagat gatgttgatt attttactgc cgtgcacatt
     1861 ggcactggcg tacctgcggg caccgtggtg tgcggcagtg ataattttat ggcaaccacc
     1921 aaatttgacg cgcacttcac cgggaccgcc gctcacgcag gcgcaaaacc agaagacggt
     1981 cacaatgcct tgttggcggc agcacaagca actcttgcac tgcatgcaat cgccccgcac
     2041 agcgaaggag cttccagagt aaacgtgggc gttatgcagg caggaagcgg ccgtaacgtt
     2101 gttcctgcct cggcgttgct gaaagtggaa acacgcgggg ccagcgacgt cattaatcaa
     2161 tatgtttttg accgtgcaca acaagcgatt cagggcgcag caaccatgta tggtgtcggc
     2221 gttgaaactc gtctgatggg tgcagctacc gccagttctc cttcgccgca atgggtcgca
     2281 tggttgcaaa gtcaggcggc tcaggtcgcg ggggtcaatc aggccattga acgtgttgaa
     2341 gcgcctgcag gttccgaaga tgccacatta atgatggccc gcgtgcagca acatcaaggg
     2401 caagcctcct acgtggtgtt tggcacacag ctggcggcag gtcatcacaa cgaaaaattc
     2461 gattttgacg agcaggttct cgctattgcc gtcgaaacgc tggcgcgcac cgcgctcaat
     2521 tttccctgga cgcgaggtat ctgatgcagg aaatctatcg ttttatcgac gatgcgattg
     2581 aagccgatcg ccaacgttat accgatattg ccgatcaaat ctgggatcat ccagaaacac
     2641 gttttgaaga gttctggtca gcggagcatc tggcttcggc gctggaatct gcaagcttca
     2701 ccgttacccg caacgtaggc aatatcccaa atgcatttat tgcttcgttt ggtcaaggca
     2761 aaccggttat cgccctgctg ggagaatatg acgccctggc aggtttaagt cagcaagctg
     2821 gttgcgcgca acctacatcc gcgacgcccg gtgaaaatgg tcacggttgc ggacacaatt
     2881 tgctgggaac cgccgccttt gcctctgcaa tagccgtcaa gaaatggctg gaacaatatg
     2941 ggcaaggcgg cacagtgcgc ttttatggtt gtcctggcga agaaggcggc tcgggtaaaa
     3001 cgttcatggt ccgcgagggg gtatttgatg atgtggatgc ggcactcacc tggcacccgg
     3061 aagcctttgc cggcatgttc aatacccgca cgctggcaaa cattcaggca tcatggtgct
     3121 ttaaagggat cgcagcacat gccgcgaatt cccctcattt gggacgcagc gcccttgatg
     3181 ccgtaacgtt gatgaccact ggcaccaact tcctcaacga acatattatt gaaaaagcgc
     3241 gcgtacacta tgccatcaca aatagcggcg ggatctcgcc caacgtggtc caggcgcagg
     3301 cagaagtgct ttatcttatc cgtgcccccg aaatgaccga cgtgcagcat atttatgatc
     3361 gggtcgccaa aatcgccgaa ggtgcggcat tgatgaccga aaccacggtt gaatgccgct
     3421 tcgacaaagc ctgttccagt tatctcccga atcgcacctt agaaaatgcc atgtaccatg
     3481 ccctatccca ttttggtacc ccggaatgga actccgaaga actggctttt gcgaaacaaa
     3541 ttcaggctac gctcaccccc aacgatcggc aaaacagtct gaataatatc gccgcaaccg
     3601 gtggcgaaaa cggcaaggtt tttgcactac gtcatcgtga aacggtactg gcgaatgagg
     3661 tcgctccata tgccgccacc gataacgtgc ttgcggcatc gactgatgtc ggcgacgtca
     3721 gttggaaact gcctgttgcc cagtgtttca gcccctgttt tgccgtcggt acaccgctac
     3781 atacgtggca actggttagc caggggcgaa catctattgc tcataaagga atgctgctgg
     3841 cggcgaaaac tatggcagca accacagtca atctcttcat tgattcaggg ctattgcaag
     3901 aatgccaaca agagcatcag caagttacgg acacgcaacc gtatcactgc cctatcccga
     3961 aaaacgtgac accgtcacct ttaaaataac aacaacgcaa acacaacaac cgaggaatgc
     4021 ccatgagtat gtcatccata ccgtcgtcct cccaatccgg gaagctctat ggctgggtcg
     4081 aaagaattgg taacaaggtt ccccatcctt ttttgctctt tatctatttg ataatcgtac
     4141 tcatggtgac gacggcaatt ttgtcagcct ttggcgtcag tgcgaaaaac ccgaccgatg
     4201 gtacgccggt ggtggtgaaa aacctgctca gtgtggaagg attacactgg tttttaccca
     4261 atgtgattaa aaactttagc ggttttgctc cacttggagc gatcctggcg ctggttttag
     4321 gtgccggcct agcggagcgc gtcggcttac tgccagcgct aatggttaaa atggcatcgc
     4381 atgttaatgc ccgctacgcc agttatatgg tgctgtttat tgcttttttc agccacattt
     4441 cttccgatgc ggcgttagtg atcatgccac cgatgggtgc gctgattttt ctggcggtgg
     4501 gcaggcatcc agttgcaggt ttactggctg ccattgcagg cgtaggttgc ggctttacgg
     4561 ctaatttact gattgtcaca accgacgtgt tgctgtcggg gatcagcacg gaagcggcgg
     4621 ctgcgttcaa tccgcaaatg cacgtcagtg taattgataa ctggtatttt atggccagct
     4681 ccgtagtcgt actgacgatt gttggcggct tgataaccga caaaatcatc gagccacggt
     4741 taggtcaatg gcagggaaac agcgatgaga aactgcagac attgaccgaa agtcagcgtt
     4801 ttggtttacg catagcaggt gtcgtatcgc tactttttat tgctgcgatc gcgctgatgg
     4861 tgatcccgga aaacgggata ttgcgcgatc cgattaatca caccgtgatg ccatcaccct
     4921 ttattaaagg tatcgtgcca ctaatcattc tttttttctt tgtggtttcg ctggcttatg
     4981 gcatcgctac ccgcacaatt cgacgtcagg cggatttacc gcatttaatg attgaaccga
     5041 tgaaagagat ggcgggattt atcgtgatgg tttttcccct cgcccagttt gtcgccatgt
     5101 ttaactggag caacatgggg aaattcatcg ccgtggggct gaccgatgta cttgaaagtt
     5161 cagggcttag cggcatcccg gcgtttgtcg gtctggcgtt gctttcctct ttcttatgca
     5221 tgtttattgc cagcggttcc gcaatctggt cgattctggc ccccattttc gtaccaatgt
     5281 ttatgctact tggctttcac ccggcatttg cgcaaatcct ctttcgtatt gccgactcat
     5341 ccgtattgcc tttagcgccg gtatctcctt ttgttccact gtttcttgga ttcctgcaac
     5401 gctacaaacc agacgcgaaa ctgggtactt actattcgtt agtcttgccc tatccgctta
     5461 tctttttggt ggtatggctg ctgatgttgc tggcgtggta tcttgttggt ctgccgatag
     5521 gtccggggat ttacccacgt ttgtcttaag agagaacgga tgctgagatt acttgaagaa
     5581 aaaattgcca cgccactggg tccactgtgg gtgatttgcg atgagcaatt tcgcctgcgg
     5641 gcggttgaat gggaagagta cagcgaacgc atggtgcagc tgctggacat ccattatcgc
     5701 aaagaaggct atgagcgcat ttctgccacc aacccaggcg gtttaagcga caagcttcgt
     5761 gattattttg ccggtaatct tagcattatt gatacgcttc ccactgccac aggggggacg
     5821 ccatttcagc gcgaagtctg gaaaactcta cgcactatcc cctgcgggca ggtaatgcat
     5881 tacggccaac tggctgaaca attgggccgc cctggcgcgg cgcgtgccgt tggtgcggca
     5941 aacggatcga atcccatcag catcgtcgta ccttgccatc gggttattgg ccgaaacggc
     6001 accatgaccg gatatgcagg cggagttcag cgaaaagagt ggttattgcg ccacgaaggt
     6061 tatcttttgc tgtaaacatt aaacaatttg tgccagcttg ttcacacttt tatgtaaagt
     6121 tacccttaac aacttaaggg ttttcaaata gatagacata tatttacatc taatatcgga
     6181 attctctgct gttaaggttt gcttagactt acttgctccc taaaaagatg ttaaaattga
     6241 caaatatcaa ttacggcttg agcagaccta tgatcccgga aaagcgaatt atacggcgca
     6301 ttcagtctgg cggttgtgcg atccattgcc aggattgcag catcagccag ctttgcatcc
     6361 cgttcacact caacgaacat gagcttgatc agcttgataa tatcattgag cggaagaagc
     6421 ctattcagaa aggccagacg ctgtttaagg ctggtgatga acttaaatcg ctttatgcca
     6481 tccgctccgg tacgattaaa agttatacca tcactgagca aggcgacgag caaatcactg
     6541 gtttccattt agcaggcgac ctggtgggat ttgacgccat cggcagcggc catcacccga
     6601 gcttcgcgca ggcgctggaa acctcgatgg tatgtgaaat cccgttcgaa acgctggacg
     6661 atttgtctgg taaaatgccg aatctgcgtc agcagatgat gcgtctgatg agcggtgaaa
     6721 tcaaaggcga tcaggacatg atcctgctgt tgtcgaagaa aaatgccgag gaacgtctgg
     6781 ctgcattcat ctacaacctg tcccgtcgtt ttgcccaacg cggcttctcc cctcgtgaat
     6841 tccgcctgac gatgactcgt ggcgatatcg gtaactatct gggcctgacg gtagaaacca
     6901 tcagccgtct gctgggtcgc ttccagaaaa gcggtatgct ggcagtcaaa ggtaaataca
     6961 tcaccatcga aaataacgat gcgctggccc agcttgctgg tcatacgcgt aacgttgcct
     7021 gatttttccg cataactcac tatccttctg tcatatcatt aaatttttct gatttattga
     7081 tctggcagaa ggttcatcac tgtttcattc accagatatg ggttaatctt ttaattacaa
     7141 actgcgttga cagttgttgt aaggagaccc tgtatggcta tgtatcagaa catgctcgtt
     7201 gttatcgatc ctaaccagga cgaccaacca gcattgcggc gagctgttta tttacatcaa
     7261 cggattggtg gcaaaattaa agcctttttg ccgatctatg acttctcata cgaaatgacc
     7321 accctgctct ccccggacga acgtaccgct atgcgtcagg gcgtcatcag ccagcgtaca
     7381 gcctggatcc acgagcaggc aaaatattat ctcaatgctg gcgttcccat tgaaattaaa
     7441 gtggtctggc ataaccgtcc cttcgaagcc atcattcagg aagtgatcag cggcgggcac
     7501 gatttggtgc taaaaatggc gcaccaacat gaccgtctgg aagcggtgat ttttacgcca
     7561 acggactggc atctgttacg caaatgccca agcccggtgt ggatggtgaa agaccagccg
     7621 tggccggaag gaggcaaggc gctggtggcg gtgaatctcg ccagtgaaga gccgtatcat
     7681 aatgcgctca atgaaaaact ggtcaaagag acgatcgaac tggcagaaca agtcaaccat
     7741 accgaagttc atctggttgg cgcttatccg gtaacgccaa ttaatatcgc gattgaactg
     7801 ccggaatttg atccgagcgt ttataacgat gccattcgtg ggcaacattt gctggcaatg
     7861 aaagctctgc ggcagaaatt cggcattaat gaaaacatga cgcacgtaga aaaaggtctg
     7921 ccagaagagg tgattcctga tttggcggag cacttacagg cggggattgt ggttctgggc
     7981 acggtaggac gcaccggtat ttcagcagca ttcctcggca acacggcgga acaggtgatt
     8041 gatcatcttc gctgcgatct gttggtcatt aaacctgacc agtatcagac acccgttgaa
     8101 ctggatgacg aagaagacga ttaacgactc ttcaataact gacaaggccc gagttatcgg
     8161 gcctattttt tacttactgc tcttcgccgc caaggaaata aattcccaac gggattgcca
     8221 gcaacacagt aaagaccagg ctataaacaa aaatcatcga tgactgaatc acatacattg
     8281 atgtcgtcca ctcagacagc ggaatattgt actgttctat aacgccgccg atagtcgcgc
     8341 gagtcacaac cgacgccgca ataatgaaaa ccgccaacaa accaaagaga aatttcttcc
     8401 ctttcgctga cttcagtttc gccataatca tgatgtaatc cttgtgcaga caaatttctt
     8461 gttacctaaa cgtaacaata ccatgacatt tcaccgttgt ctgctctgaa aacctcacct
     8521 cttagctgga tttacaatca acgcattgat tagttagcta atgttgctac ttccttccct
     8581 gactattgat gatctgaatg aattgtcgcg gtaaaatagc cccgccaggg gtttccggca
     8641 tgacgacaat tatctggagg agcctgataa aacaatgatc gctgaactgt ttacaaataa
     8701 tgcgcttaat ctggtcatta ttttcggtag ctgcgcagca ttgattctga tgagcttttg
     8761 gtttcgccgc ggaaatcgta aaagaaaagg ttttttattc catgcggtgc aatttttaat
     8821 ctacaccata attatcagtg ctgttggtag cattattaat tatgttatag aaaactataa
     8881 actcaaattt atcaccccag gcgttatcga ttttatctgt acgtccctga ttgcggttat
     8941 tctgacgatt aagctatttt tgctgattaa tcagtttgaa aaacagcaga ttaaaaaagg
     9001 tcgcgatatc accagtgcgc ggattatgtc gcgcattatc aaaatcacca ttattgtggt
     9061 gcttgttctg ctttatggcg aacatttcgg catgagcctt tctggcttgc tgacctttgg
     9121 tggtattggt ggtctggctg tcggtatggc cggtaaagat attctgagta atttcttttc
     9181 cgggatcatg ctctatttcg accgtccttt cagtattggc gactggatcc gttcaccgga
     9241 cagaaatatc gaaggtacag tagcggaaat tggctggcga attaccaaaa ttacgacctt
     9301 tgataatcgt ccattgtacg taccgaactc gctgttttcg tcgatcagcg tagaaaaccc
     9361 aggacgaatg accaaccgcc gcattaccac gaccattggt ttacgttatg aggatgcggc
     9421 aaaagtgggc gttattgtcg aagccgtacg tgagatgctg aaaaatcacc cggccatcga
     9481 ccagcgacaa accttactgg tttatttcaa ccagtttgcc gactcgtcat tgaatattat
     9541 ggtttattgc tttaccaaaa ccacggtatg ggctgaatgg cttgctgcac agcaagacgt
     9601 ttatttgaag attatcgata ttgtgcagtc acacggcgcg gactttgcct tccccagcca
     9661 gacgctgtat atggataaca ttacaccgca ggaacagggg cgctaaatca gacatcactg
     9721 gcgttatcca caagggtaac gccagttccc atcacatcaa tgcttcacaa tatacatagt
     9781 ccggctgtac gccacatctt ccgggttatt aatgggataa cctttcagcc acggcttgat
     9841 taatcgtcca ttggtatatt gataaattgg tgcaatcggt gcttgctcca tgaggatttt
     9901 ttctgccgcg ttgtaatcgg cattacgcgc tttaacggta ttttccgtcg atgcctgggc
     9961 cagaactttg tcgtatgccg gattgttaaa gcgtgaaata tttcctgaat gcgttgacgt
    10021 taataatgtc aggaaagtgg agggttcatt ataatccccc acccatgagg cgcggataac
    10081 atcaaaattg ccggtgttac ggctatcgat ataggttttc cattcctgat tttgcaattt
    10141 aacatctacg ccaaggttct ttttccacat cgatgctaca gcaatcgcaa ttttttgatg
    10201 gttttctgaa gtgttataca aaagcgtcag cttcagcggt ttttgcggac cataaccagc
    10261 tgcgctcaac aaagtttttg cctgcgcatt cagttcttcc tgactcattt gttcaaacgg
    10321 cgaaggttcc ggcgtaaatc cggcggtaac atctggtgta aaatgccacg ctggcttttc
    10381 gcccgtccct aatacttttt cggtcatcag gcggcgatct atcgtcatac ttaatgccag
    10441 acgaacgcgc tgatctgccg tcggcccttt ttgcgtgtta aacgcataat aataggtccc
    10501 gagctgcggc ggcgtataaa cctgccccgg aatatccttc aacagcttct gatacatatt
    10561 tttcgggaag gattcggtga tatcaatatc ccccgcgagg taacgcttag tggctgcgga
    10621 ttcctgatta attggcagga aggtcacttt ttgcagtacc gttttggcgt tatcccaata
    10681 atgggtattc ggtaccacga ccagtttttc attgactacg cgttctttaa gaacataagc
    10741 gccattgccg atcagatttc cgggtttcgt ccactcttta ccgctttcta cgttggcttt
    10801 ttgcaccggg aagaaggcaa agttagcggt taaattcaca aaccacggca acggtttatc
    10861 aagctgaatt ttcaaggtat gggcatcaac tgcggtgacg ccaagctggt caggcgtagc
    10921 tttaccatca ataatcgcct gtgcgttgtt gattcccgcc agcgcggcaa accatgcaaa
    10981 tggcgatagc gttttcgggt ccaccagacg ttgccagctg tagacaaaat cttgcgccgt
    11041 taccggtgtg ccatccgccc attttgcgtt atcgcgcagg gtaaaagtcc agatacggtt
    11101 gtcattactt ttccactgag tcgcaacgcc ggggacaatc tcccctttct cgttctgatt
    11161 caccagtcct tcaaacagat cgcgaatgac ctgaatctct ggcaggccca cggctttagc
    11221 gggatccagc gacgcaggct catctttaat gtggcgcacc agctcctgct tctctgccag
    11281 tactgtgccg ctcggaactt ctgcagcata cgaaagagaa atgctgctga ccaacagcgc
    11341 acaacacgtg actgaaacag agtgcttcat aagtttccct ctaaccgaca ttaaaattga
    11401 tgcgtaatta tttgtttcgc cttaaagaaa tgcaaacaac ttccatcaaa aaagtgacac
    11461 aggaagcata aaatgtttta tgtatcaata tattaaatca gatttagtgt aaataatcga
    11521 cgccaaaatg cggcaaagca catagaacac cgaacactac gttcaggctt cgcacgttca
    11581 aaccagtttt cggtatcaaa atcagcatct gtcgccattg tacgaagaat aaaaaataac
    11641 caaaacgttg aaaggaactg agaatacgtt aagagagaaa ttaaattaat gtatcagcaa
    11701 gtagtgctaa acagcacctt tatacttcag cctatctata aataatgaga atgccgagcc
    11761 agcatgtcga cggtgtggat agtacaggtg ataaccgggt aaatctggtg tgaatttatc
    11821 caaaacacgt atcagttttt tttctttaat agcccgctca acctgatcat aaggtagata
    11881 cgccaatcca tgcccatcaa ttgcagcatc aatgatcagg tctatcgtat tcagtaaaag
    11941 ctgaccttcc atgcgaacac gaacttcacg tccaccgcgt attaatctcc agcgatttgc
    12001 tgtacccgat gtgggaagat acaaattaat tgcctgatga tctattaatt gtgacactga
    12061 cgttggaaca cttcggcgag aaaaataatc cggtgagcca acaatagcca ttggtatatc
    12121 tggcccgatt cgaatggcga tcatatcttt atccatctcc ccacccagac ggacgcctgc
    12181 atcaaaacgt tcagaaacga catcggtcaa accataatca atggtgagct gaatatcaat
    12241 ttcaggatgc gatttcagga atgtgcgcat tgctggtaac aatatcgttt ttgctgcatg
    12301 ttctacagta gtaatacgta ttgtcccgga tgggcggttc tgcagatcgc tcagggatgc
    12361 catggctgaa tctatgtcat gcaacatagg gccaagaaca gacaaaagat gctcgcccgc
    12421 ttcagttgga acaacgctgc gcgtggttcg cgtcagaagc cgcaatccca atcgttcttc
    12481 tatacgacgc actatctggc ttaaagctga ctgcgccatg ctcaggcggg ctgctgcacg
    12541 agtgaagcta cgctcctctg caacgacgac aaacgccatc agatcagcga tttcttcacg
    12601 cttcattcag tatctccatg caatctatta atcatttaaa tgataggtct aatcatgatt
    12661 ttgggtctaa tcaacttaat gatcaatatc tatagtgata tcaccaaccg ttaaaacgaa
    12721 ccgacaactc catgaggaac ggaatgatga ataataaagt cagcttcact aacagcaata
    12781 atccaaccat ctctttgtct gcagtgatct atttccctcc aaaatttgat gaaacccgtc
    12841 agtatcaggc aattgtgtta tctcacccag gtgggggcgt taaagaacaa acggccggaa
    12901 cctatgccaa aaaactggcg gaaaagggat ttgtcactat tgcttatgac gcatcttatc
    12961 aaggtgaaag tggcggcgag ccgcgtcagt tagaaaaccc atacattcgt actgaagaca
    13021 tcagtgccgt gattgattac ctgacaacgc tttcttatgt cgacaatacc cgaattggtg
    13081 cgatgggaat ttgtgccggt gcaggatata cagccaatgc tgcaattcag gatcgccgca
    13141 tcaaggccat cggtaccgtt agtgcggtca atattggttc gatgttccgt aatggttggg
    13201 aaaacaatgt gaaatctatt gatgccctgc catacgttga agcgggttca aacgcccgaa
    13261 ccagcgatat tagcagcgga gaatatgccg ttatgccact ggcaccaatg aaggagtccg
    13321 atgcgccgaa cgaagaactg cgtcaggcct gggagtatta ccacacccct cgcgcgcagt
    13381 atccaacagc accgggttac gctactttgc gcagccttaa ccagattatt acctatgatg
    13441 cttaccatat ggcggaagtg tacctgactc agccgatgca gattgtggcg ggcagccagg
    13501 cagggagcaa atggatgagt gacgacctgt acgatcgggc atcaagccag gataaacgct
    13561 atcacattgt tgaaggcgca aaccatatgg atctttatga cggcaaagcc tatgtggctg
    13621 aagcaatttc agtattagct ccgttctttg aggaaactct gtaatgaaac atattcaaat
    13681 ccgcaatagt gatatggcct ggcatattgc tgccaatatt cagtttccac caaattttga
    13741 tgagtcaaaa cagtatccgg cgattatcag cgtccatcct tttggcagtt gtaaggagca
    13801 gacgtctggc aatatctatg gcaaagcatt ggctgaaaaa ggctatctcg ttctcgctta
    13861 tgacgccagt ttccagggag aatctggtgg ggagcctcgc tggatagaag atccgacaca
    13921 gcgcgtagag gatattagcc gtgttatcga ttatgcagta actctgccat atgttaatgc
    13981 tgaacgtatt ggcgtgctgg gagtgtgtgg gggtggtggc tatgccatta atgccgcgct
    14041 gacagaaaaa cgaattaaag cagtagtgag tattacgggt gttaatatcg ggcgtctttt
    14101 tcgggaaggt ttcagcaatt acgatccgat tggcgcattg aatgcaatgg cgtcgcaacg
    14161 aaccaaagag gctcgtggtg gagaattaca gatcaacgaa ttacttcccg ccagcctgga
    14221 tgcagcaaaa gcccatggac tgaccgaacg cgatgtttat gaagcaactg actactataa
    14281 aacaccacgc gggcaacaac cgggaggtgc gacaaaaatg ttgttctcac atgcacagaa
    14341 aacgctggcc tgggatgcct tcgcatttac tgaagtgtta ctgactcagc cggtgatggt
    14401 agtagttggt gaaaaagtcg gtgccttcgg tgcttatcgt gatggactgg aagtctatgg
    14461 ccgggcgatg gtttcgcagg atcgtcagtt agtttcatta cccgatttct cacattatga
    14521 actgtatgac aaaccggaag cagttcagga ggcgctggaa aaagttattc cttttttcaa
    14581 cacccatctg ggataagttt catgagaaat attatgaaaa atgtactgat tcttggtgct
    14641 ggcggtcaga tagcccgcca tgtgattaat caactggcag ataaacaaac cattaaacaa
    14701 actttatttg cccgtcagcc agcaaaaatc cacaaaccct atccaactaa tagcaagatt
    14761 attatgggag atgtactgaa tcatgcagca ctgaagcagg ccatgcaggg acaggacgtt
    14821 gtgtatgcca accttacggg ggaagattta gatattcagg caaattcagt gatcgctgcg
    14881 atgaaagcct gtgatgttaa acgtctgatc tttgttcttt ccctcggtat ttatgatgaa
    14941 gttccaggaa aatttggcga atggaataat gcagttattg gtgagcccct aaaaccattc
    15001 cgcagggctg cggatgcaat tgaggcttct ggactggaat atacaattct tcgccctgcc
    15061 tggctgactg atgaagatat tattgattat gagcttacct cacgcaatga accattcaaa
    15121 ggtacgatcg tatcacgtaa gagtgtcgca gcattaatca cagatatcat cgataaacct
    15181 gagaaacaca ttggtgataa tataggtatc aatcaacccg tgactgatgg tgacaagcca
    15241 ttattcatgt aaatcaatta catagttacc atcgtaatgt actatgtgca gtacgattta
    15301 ttaaacttat taaggtgaac tgatcaatcc gatgcgatta acatgtgtag aaccagtaag
    15361 aataaaatac gccatgtata taacattacg acttacatct aagtcagcat aattctaata
    15421 tctgcacatc aataaaaatg caaacaactt ccggcaaaaa agtgatacaa gatcgatgac
    15481 atagcgttga agaaaaggat ttgataatcc gcaaaatttg cacaacactg cctgtaatac
    15541 ttcctgaacc agagacgatc atgaccgtaa accgcccacg cgccgaacgc ggcgcatttc
    15601 cgcccggaac agaacattac ggacgttcat tattgggcgc gccgttaatc tggtttcctg
    15661 cccctgcagc cagtcgcgaa agtggtttga ttctggctgg cactcacggt gatgaaaact
    15721 cttcggtcgt cacgctctcc tgtgctctgc ggactttgac accttctttg cgccgccatc
    15781 atgtggtgct gtgtgtgaat cctgacggct gccagctggg gttacgggcc aatgctaatg
    15841 gtgtggattt aaacagaaac tttccggcga cgaactggaa ggaaggtgaa acggtttatc
    15901 gctggaacag cgccgctgaa gaacgtgatg tcgtattact gacaggtgac aaacctggct
    15961 cagaacctga aacccaggcg ctgtgccagc ttatacaccg cattcaacct gcctgggtgg
    16021 tctcattcca cgatccactg gcctgtattg aagatcccag acacagcgaa ttaggcgaat
    16081 ggctggccca ggcgtttgaa ttgcctctgg taaccagcgt cggttatgaa acgcctggct
    16141 ctttcggcag ttggtgtgca gatctcaact tgcattgcat caccgcagag tttccgccca
    16201 tctcctccga cgaagccagc gaaaaatatc tctttgcgat ggcaaacttg ctgcgctggc
    16261 atcctaaaga tgcaattcgc ccgtcgtgaa ctgaagcgcc ggttccacat ctaccgccag
    16321 ccaggtcgat ccgtcaagat cggcgaaact gacctgcggc accagcggta aagcggcgct
    16381 aatggcacga gaggtacaca acatgcagcc cagcatcaga ctgaaacctt gtgcacgcgc
    16441 ttcagtcgcc agcgccagcg cttccgtcag accaccggtt ttatcgagct taatgttaac
    16501 catctcatag cgccctttca gcgccttcag attgctacgt gtatgacaac tctcatcagc
    16561 acaaatcggc aacggatgaa taaaattctc cagcgccgca tcgtcctgcg caggaagggg
    16621 ttgttcaagc atcgcaacgc ctaaatccgc caatagctgg caacgcgccg ccaacccttc
    16681 tgcacgccag gattcatttg catcaacgat cagcgtcgca tcgggcacag ctgtgcgaat
    16741 tgccaccatc cgctcactaa taagatggtt atccagcttc actttcagta atttcgcgcc
    16801 tgcctgccag agtgttgatg cactattggc catctgatca ggcgtaccga tgactaccgt
    16861 ctgtgcagtg ataactgtct cgggaagcgt tatgccgatc aaatcagcca gcgattgctg
    16921 ctgttttcgc gcggccagat cccacaatgc acaatccagc gcattacgtg ctgcgccggc
    16981 agggagaatt ttttgcaact cctcccgtgt cagccctttc tctaattgcg acacgacgct
    17041 cataatttgc gccattaccg aggcatcact ttctccataa cgcggatacg gcgtgcattc
    17101 gccggtgcct ttaataccct cttcttccag ttcaaccacc accacgcgcg cttcactgcg
    17161 acttccccgg gcaatcacaa acggggtatg taagggccag gcttcctcaa ataccttaac
    17221 ggttctcatc tttactcctt gttgtcgcgg tggtctgcgg attaagcgca aatagcgttt
    17281 gctgtgttat tgacagttag cataaactag gtgtgacgtt aactatatgt aaacaggaaa
    17341 gataatcatg tcacaaaccg ttcatttcca gggcaacccg gttacagtcg ccaattccat
    17401 cccgcaggcg ggtagcaaag cgcagacttt tactctcgta gcaaaagatc tgtctgacgt
    17461 caccctcggt cagtttgcgg gtaaacgcaa agtgctgaac attttcccga gtattgatac
    17521 cggagtttgc gccgcatcag tacgtaagtt taaccaactg gcaaccgaga tcgacaacac
    17581 cgttgtgctg tgtatttctg ccgatctgcc gttcgcccag tctcgtttct gcggcgcaga
    17641 aggtctgaac aacgttatca ccctctccac tttccgtaac gctgaattcc tgcaagccta
    17701 cggtgtggca attgctgatg gcccactgaa aggtctggca gcgcgtgccg ttgtggttat
    17761 tgacgaaaat gacaatgtga ttttcagcca gctggtggat gaaatcacta ccgagccgga
    17821 ttacgaagca gctctggctg tactgaaagc ataatttaat atgcctgatg gtgttgcacc
    17881 atcaggcata ttcgcgctta ctcttcgttc ttcttctgac tcagaccata ttcccgcaac
    17941 ttattggcaa tcgcggtatg tgaaacgccg agacgttttg ccagtttgcg cgtgctggga
    18001 taattgcgat aaagctgggt taataccgag cgttcaaaac ggctggtgat ttcgtccagc
    18061 gaaccttcca tcgcatcttc gcccacggct accgttgcgg cgtcataatc cggcaacaaa
    18121 atatcctgtg gacgcagctc ataaccgtcc agttgtgtca gtgcgcgata gatagcgttc
    18181 tttaactgcc gcacatttcc cggccacgca taacgcgtaa gtacagtatt caggtcagcg
    18241 gccagtttcg gacgcggcac gccctgctcg tcggcaaagc gggcgacgaa cagctcagtt
    18301 aacggcatga tgtcctgcgg acagtcacgt agcggcggca gattgagcgt caacacgttc
    18361 agacgataat agagatcttc acggaacatg cctttttgca ccagttcgac cagattcttc
    18421 tgcgtagcgc aaatcacccg cacatcgaca tgcacctcat ggtcttcgcc aacccgacgg
    18481 aaagtgccat cattaaggaa acgcagtaat ttcgcctgca tccgtggtga catttcccct
    18541 atttcatcca acagcaccga accaccgttc gcctgctcaa agaatccttt cttcccttcc
    18601 ggagcatgac caaacagttc actctcgacc gcatcttccg gtatagacgc acagttcagc
    18661 gccaggtaag gtttgcccgc tctggggctt gcctgatggc aggcgtaggc aaagagatct
    18721 ttacctgtac ctgtgtcacc cgtaatcagc agcggcgcgc ttagcatcgc cagtttctgc
    18781 gcctgttcga caacatgctt cattttcggg ctgacggcga caatttgact gaaggcgctg
    18841 acgtcctggg cggcgacatt ttgcaactgg cggcccatac gaatcgttga tcgcaacatc
    18901 accaccgcac cggtcaggac gtgttgatca ttttcatcct gaagataaac aggcgtaatc
    18961 tccatcagga aattctgccc attaataacg acatgctcgt tatgggaatc ttgcggttcg
    19021 ctttccagcc aacgtaaaaa attaaagccg ttaatcaatt gtgcggcggt atggttgcgc
    19081 aggcgatcca atttttgcgc aaaaagctga cagctcgccg ggttcgccat atccactttg
    19141 cttttcatat cgacagagag cacaggttcg ggcaacgcct ccagtaacgc gctcaacgcc
    19201 agatgctcac gttcggaagg catccacggg acagtacgca catcggtaac acctgcaata
    19261 cggcgtattt cggccatcag actgctgaaa ctctcaaact ccagttcagc aaaattgagg
    19321 tagattcgcc caatgggatc aatctcaata ccgcgtaaat caatgcctct tagcacgagt
    19381 agatcgagta attcgcgggt cagaccgagt cggtcttcac aaaagacttc cagacgcatg
    19441 ggaaccttca cctgaaaaaa gaacaattaa tatgatgata tgacactatt tggatagcag
    19501 gaaggtttct gtcaacaatc attgacacaa agacggaaaa acgggcactt cggcgtaaag
    19561 atatccggtc agcacccgat attgcattat ttttcgctgg gcgttttgcc tttttgcagc
    19621 gtttctttca cctgaccgat aagctgacga cggaaatccc cgaggcgagg tttgtcatcg
    19681 tcaatccacg gcagcgggcg gcaaagctcc atagctttaa tcccgagtcg tgccgtcaga
    19741 agtccagcac caattccctg agctgcgcgg gtagacaaac gagcagcgag atcttgcgac
    19801 atccagtcca tccccacttc gcgcaccagt tcgctggctc cggcaaaagc gatattcagc
    19861 aataccagct taaacagacg caaacggctg taatacccca gttcaatgcc atacagcgtg
    19921 gcgatgcgat taattaaacg cagattgcgc caggcgataa acgccatatc gaccaacgcc
    19981 agcgggctga ccgcaatcat caacgttgat tccgccgccg aacggctgat ttcgcgccgc
    20041 gcctgggcat ctaaaactgg ctggaccaaa tgcgcataca aactgaccac ttcacggtcg
    20101 ttttgcgttt catggattga ggcataccag cgttgcagcg ctggatgaga ctgatcaata
    20161 cccgcctgct gcgccagttt ttcgcaaaat gcgcggcctt tgcccgtgcc gtggctgtgc
    20221 aacaaatcgc gcgcttcgtc gcgttcatgg gcgcgctgtc gcaagcgcca taagcgccgc
    20281 cactctgtta ccacagaacc tacgccagcg ccgataatca atgccccagc ggcacatcca
    20341 cccagcgcca cccagtcctg agtttgccag gcattcattg tccactgtac accctgcccg
    20401 acaacgcttg cgccaaacag agccagcccg cccatcacca ttttgcgcca caggctgcgt
    20461 ttcggacgta acgctgcatc cattaccgct tcaacttgcc cctcttcttc aggcgcttca
    20521 tcgagcgtgg ccggggcaaa attttgcgcc tgattttcgt caaaggtctg ctgcgccctg
    20581 aattttggat tctgatcgac ctccagcgga ccgtcgaaat caatacgtgg ttttaacggt
    20641 tcggtcatcg caatttatct cctattaaaa attcaagcgc ggcatcaaga cgaatatgcg
    20701 gcagcggttt gtcgacatcc atcacctgcg ggcgaaacgc ttcaaactgg aacccttgct
    20761 tgtcccagaa cgcctgaccg ggcaaacgcg caggaacttc accgggataa acggtgagtg
    20821 gcgcaccatc gctgagccgg ttaccgcgca atgccggaat tttctcgccg ttgacgtcaa
    20881 taatgccgct ggtggtcgcc tgcaccgagg ccagccccag gcaatccata ctaatccctt
    20941 caaacgccgc attttgccag gcatcctgaa tcagttgttg cagtaatgaa accatattgg
    21001 cgtgctgatc gttggtcaca tggtccgctt tagtggcagc aaacaatagc ttatcgataa
    21061 ccggcgaaaa caaacgccgg aacaaggtac gctgcccgta gtggaaactt tgcatcagct
    21121 gcgtcagtgc cagacgcata tcattaaatg cctgtggccc actgttgaga ggttgcaggc
    21181 aatcaaccag cacaatctgg cggtcaaagc gcagaaaatg attcttatag aaccccttca
    21241 ccaccttctc gcagtaataa ttaaaccgct cgcgcagcat tccggcattg gtgtgcttat
    21301 cggcctgcgc cagtttggac tcgccccagg catcgacatc cggccacggg aagaattgca
    21361 gcgcgggcgc acctgccata tctcccggca agacaaagcg cccaggctga ataaagtgca
    21421 gcccctgctg tttacagtgg tggagataat cggtccacgc ggcggcaatg tctgccagcc
    21481 ggttttcgtc ggcaggcgct agcgggtcca gcccttcgca catcattcgc catttgaccg
    21541 accattcgcc gcgctgacca ttgagtaagc ccgtcatctg gcgtgaccag cttaaatagt
    21601 cctgcgccag catcggcagg tcgagcaacc attcgccggg ataatccaca atctccagat
    21661 acagcgtgga ggtgtccttg aagtggcgta gcagcgaatc gttcgattta aaacgtagcg
    21721 ccaggcggat ttcactgacg ccgcgcgttg gcgtcggcca ggcgggagga tcgccatata
    21781 actgcgccag tccttcatca tatgtgaagc gcggaatgcc aaagtcacgc tggggaatgc
    21841 gtttcacgcc cagcaggcgc tcttcacgca ccgcacttaa cagcggcaaa cgtgctccgg
    21901 cgtgaatatt aagcaactga ttaaccatcg cggtgataaa cgctgttttg ccgctgcggc
    21961 taagtccggt tacagcgagg cgcagatgtc tgtcgacacc ccgattcacc agcgcattga
    22021 gttcattttt aagtcgcttc attgctgtcc tgtgctactc tgaagaccag aaaaaaaatc
    22081 attcgtaagg atatgaatgt gggggccagg ccattgatta tcaactgccc gtgttgtcat
    22141 cgaccggcga actccataag atgtgcagca ttcacgttcg gacggagttc ctgacctatg
    22201 tcccctacta tttatgatat tgccagggtt gcaggcgtat caaaatccac cgtatcacgc
    22261 gtgctgaata agcaaactaa tatctccccg gaagcgcgcg aaaaagtgtt acgggccatt
    22321 gaagaattac agtatcagcc aaacaagctg gcccgcgcgc tgacctcttc gggttttgat
    22381 gccattatgg tgatttctac ccgttcgacc aaaactacgg cgggtaatcc gtttttctct
    22441 gaagttctac atgccatcac cgccaaagcg gaagaagaag gtttcgacgt gatattgcag
    22501 acgtcgcaca acctggcaga agacttacaa aaatgtgaaa gcaaaattaa gcagaaaatg
    22561 attaaaggca ttattatgct aagttcgcca gcggatgagt cattttttgc ccaacttgat
    22621 aaatacgata ttcctgtcgt ggtgattggc aaagttgaag gtcaatatgc ccatgtttat
    22681 tctgtcgata ccgataattt tggcgacagc attgcgttga ccgatgcatt aattgaaagt
    22741 gggcatcaaa atattgcctg cctgcatgct ccgcttgatg tccatgtttc agtggatcgg
    22801 gtaaatggtt ataagcagag cctggctgcg cataatattg cagtgcgtga tgaatggatt
    22861 gttgacggcg gttataccca tgaaacagca ctgcaagccg cgcgggaatt attaagccag
    22921 tcaccgttgc ctgaggcagt gtttgctact gacagcctga aattaatgag tatttatcgt
    22981 gcggcagcag agaaaaatat tgctattccg cagcagttag cggtggtggg ttatagtaat
    23041 gaaacgctgt cgtttatttt aacgcctgcg ccaggcggta ttgatatccc aacacaggag
    23101 ttaggtcaac aaagctgtga gttattattc cgcttaattt ctggaaaacc gtcaccacag
    23161 aatattaccg ttgccacgca tatgacgctg aaataaatac cagctacctt gcctgaatgc
    23221 cggatgcgat gctagcgcat cttatccggc ctacatttat agttcggata aagtactcgt
    23281 gccgcatccg gcaatttagc ccattatcag aacgagtaat ttacgccgac acctgcataa
    23341 tggaatttat cgctgtcgcc ttcgtcgtga tcctgccact caaacgcgta ttccagcgaa
    23401 acggaaagcc cgttctggaa atcataaccg taaaataaac ctacacggtt aaaatcatgg
    23461 ccttcacgtt caatatcatc ctgccagtcc cagttactcc agcgatccag cccaatgcgc
    23521 gtatacggcg tcaccgagtg gttgccgagc gtcagcggca aataggcgcg aatttcttgc
    23581 gtagaaaact caccgttatt gcggctgtcg tccatattaa agccgcgctc gagataatag
    23641 ttcactcgca aggcaaccgt ttcgttgaag gtatattgca ggcctgtttc cgtttcgact
    23701 cgggtatcag cgtaaccggt cgtgttcaga tcgttggcaa atttatacat cgacaaccaa
    23761 ccgttgaaac gtaaatcgtc agtcagtttc acatcccagt ctggcgcgat tttccagcgc
    23821 tgcatattcg ccgtgtcttt acccggttca tcaacgtagt gataaccata attacggaaa
    23881 ccgccggtca ggccgaaact gaaatcatcg ttttcgagga actgataatg cacctccagc
    23941 tccgggcgat caaaccacgt tccacgttta cccgcgctat aatctaccgg cccttcctga
    24001 taataggcca gagcaattct ccacggcccg ttggcggcat taaaatagac tgaaggctcc
    24061 gccagcccat ccatatcttc gccataaccc tcgacgtttt ctatttcgta catcgcgccg
    24121 atattaaagt gccagtcgtt cctttcctcg gcctgtgcgc aggccattcc cgcacacatc
    24181 accagtgcgg tacagggtaa taactttttc attattgttc cttgtattat tctgggggaa
    24241 tgccttcccc ctgtatttag cgaattgcta tttccgtttc tgcatcaaag aaatgacatt
    24301 tcgtcatatc aaaatgaata gtgatatttt ctcctgcatg ataatcattt aacgcaccag
    24361 cacggaccac taactcgtgc cccccaaccg tggtgtagag cataaattcc gcaccggtta
    24421 attctgccac gctaattttg gcggaaatgt tattttcctc ttgcgcgtcc ggatgaatat
    24481 cttccggacg tattcccatc acgatgggct tatgcaaact tccctgtgtt ttcagaaccg
    24541 ctaatttctc ttcgggaatg gttaatttaa gcgtttccgt aacgaattta tcgccatcga
    24601 tcgtgccacg aataaaattc atcgccggtg atccaataaa tccggcaaca aacatattcg
    24661 ctggttggtt ataaaccgtt ttcggcgcac caacttgctg aacaatccca tccttcataa
    24721 tcacaatccg cgtcgccatg gtcatcgctt cggtctgatc gtgggtcacg tagatcatgg
    24781 tggtattcag cttctggtgt agcttgctga tctctgcgcg catttgcacg cgcagcttgg
    24841 catcaaggtt agagagcggt tcatccatta aaaacacgcc cgcttcgcgc acaatcgccc
    24901 gcccgagcgc cacacgctgg cgttgcccgc cggaaagcgc gcccggcttg cgtttcaggt
    24961 actcacgcag gccgagaatt tgcgccgccc agttcacccg ctcatcaatc acctctttgg
    25021 cgattttttg catcttcagg ccaaacgcca tgttgtcgta aaccgtcata tgcggataca
    25081 acgcgtagtt ctggaacacc atcgctatat tgcgtgcttt ggctggaacg tcattcattc
    25141 gtttgccgtc gatcaacaga tcgccgccgc tgatctcctc aagcccggca atcatgcgca
    25201 gggtggtcga cttaccgcag cccgacgggc cgacaaacac gatgaactct ttatcggcaa
    25261 tttccaggtt gaagtccttc accacatgca cctggttatc gtagattttt tgaatatgtt
    25321 gtaacgaaag ctgagccatg ttgattcctt tgctatacgt tttgccagaa ggccgataac
    25381 cgcgaccagg tcagagagtc cgttgaaggc aacagtaatt gcgccccggt taagcccgcg
    25441 ccgatcccca ccgagcgcat accgctggcg ttaatggcgt caatgcccgc ctgcgcatct
    25501 tcaatgccga tacatgcctg cggcggcacg cccagcccgg cacaggcggc gagaaagatt
    25561 tccgggtccg gtttcgagtt tttaagttgg gaagcatccg cgcagaaggt gaaaaactcg
    25621 cgcagctcca gcgccgctaa aatcgtcggc gcattcaggg agacagaagc cagcccaacc
    25681 gggatctgct gtgcacggag atctgccagc aaagagcgaa tgccgggtag aacagcgctg
    25741 accgtcaact cgcgcagtga gtggacatag agcagatttt tgcgatacgc cagttgcgcc
    25801 ctctcctgcg agttaaagtc gccctctttg cccccgtgtt gcagaatgcg ccgcagagac
    25861 tcatcgcggc tgatcccttt tagggattcg ttaaactgcg catcaatgct gatgccaatt
    25921 tcagcggcaa tctgctgcca cgcctggaaa tgcagatgcg cggtatcggt gattacacca
    25981 tccagatcga aaattacccc ttgcagtttc attcatcctc ctgatgtgtg gtagcggtcc
    26041 cattgaaggg caaaataaaa tcacccaaac agaaaacaga ttcttcagcc acggatataa
    26101 gctgaccgtt caaacgcagt gaaacgggcg cagaagttcg aatcgcaata cgctgcgcgt
    26161 caagagtgac ctgtaattcg cagccctgcc agaacagagg gaaagacaac tgttgccact
    26221 gctcaggtaa cgccggattg agatgcaatt caccgtcacg cacgctcacc ccggcaaaac
    26281 cctgaatcgc ccccagccag atagcgccag ttgcggcagc atggatacca tcatcacaac
    26341 tatgcggatc agcaccaaga tcgatttcag tcccctcgcg ccagaactga taactttggg
    26401 tcagcaggcc acagcgtgcg gcaacaatgc cgtggattgc tttacttaat gacgagtcgt
    26461 gaatagtgcg cggttcataa aattgcagat tggcaagaca cgatgccgct gagaactgct
    26521 ccggcagcat gtaattgagc atcaccacat cagcttgttt gaggatttgc atctcgttca
    26581 cttctgcgcg tgaataatcc agcagaatgg tttgcttccc cgccgccgct ttgtatttcg
    26641 ccaggttaat cgccggctta gccataaacg aatcatcctg cggcaaaacg ccgtcgggct
    26701 gaatttctgg cagccgcagt tctttaagga acatttcggc acgatgaata aacgcgtcgt
    26761 cgctacagcc aaactggcgg gcaatactca gcgcctgctg gacgttgtaa tacgccatat
    26821 agctggtgaa ggcgttatta ttgacatgtt cggtatattc gtcaggccca ataacatcat
    26881 gaatttccag acggtcgtta accctcaccg cgcggctaat ccagaacttt gcagtttcca
    26941 gaagtagcgc catgccttca tgcgcaatga aactttcatc ccccgtggtc tgccagtatt
    27001 gaataaccgc ccaggcgata tcggccacca gatgatgttc cgcctgcgcc gaggccactt
    27061 tttgccgcag cccggtgcga atgttaatgg cggcaaaaag tggcgtctct tcttcgccgc
    27121 tgcgcgcgct ttcccacggg aatagcgcgc cctgccagcc attgcgtcgc gctttctcct
    27181 gcgcgccagg caagttgtgc cagcgataac gcagtaaact tctggcaatc gtcggatcgc
    27241 taaacagatg gaacggcagc aaaaatactt ccgtatccca gaaaacgtgg cctttgtagc
    27301 cttcgccggt taagcctttt gccgcaatgc tgctgcgctc gtcgtgggca ggcgtcatga
    27361 tgcgcagatg ataaagcgca taatctaacg cttgctgatc gtgcgcttcg ccgccattta
    27421 ccgtgatacg acgtttctgc caccattgac gccagttttc tgttgatact gcaagaagtt
    27481 ggtcgtaact ctgctgcgcg cacatttcaa gctggcgaag cgacgcgctg ccccactcgt
    27541 ctaaaacggc ttgcctgtca tcccgccagt cgatccacac cagtttttgc aacgtcactg
    27601 tctcgcctgc atgaagctgc gcactggtat gttgctgcaa acggcgctct ttggcggtat
    27661 agcattgctg cacatcaccg ctcaccatac agcaacagct gatggccaca tcactgcgtc
    27721 catcctgggt ggtgtagatc ccctgcatca gatgctgacc aaacacccgc acctgggttt
    27781 cgtcgagatg ttggcgaccg tggttggttt gcgtggcgtc gatacctgtt gaaatcagca
    27841 ctgacgcgtc ggcgtccagt ggcgtaatag tgatttccag cacaatgagc ggcagttggt
    27901 ctgccgaaac aaagcgacgg ctggcgatgg tgtaacctgt gccgttgctg gtacgccaga
    27961 caacgctacg gcgtaattct ccactggcaa agtcaagttc acgctgccag gcttcgcggg
    28021 ataacgagaa aacctcacca tttatggcaa tctccatccc caagatatca ggcaggttca
    28081 ccagttcgtt gatttcacct tttcccgccc gatgatacag ccccgccaga tacatccctc
    28141 gcgtctggcg cgtgtaatct tcttcatggc tggcgcgaag cccaagatag ccgttcccct
    28201 gcgccatcag cgatgcatac ttgttcaggg tatgctggct gaaatggggt tctgataacg
    28261 ttactggcct ggtcataatt ccacacgcgt ccctgtttct gcggattgat acaacgccgc
    28321 caccagttgc tggatgatgt acccctgctc ggcgtcggct atcatcacgg gcttgccctg
    28381 tacgtgattg ataaaggctt ccatgctgcg caaatggcgg ttgtcgtctg ccagttcccg
    28441 ttgcatcagc gtcattaatt caccgttgtt atcggtgtag atatgtgctg gaaacagcgt
    28501 cgcaccggct ttatcaccac aaaagctgac gttcataatc gactgttcgc ggatgttgag
    28561 tgcaaatgac gtttccagcc acagaatgcc gccgttatga aattcaatgg tgccaaacag
    28621 cgaatcttcg acgctataag ttgccggatc ccactcgcca aattgaccac agctcttttg
    28681 cgtgccgatc ttttgaaagc tatgcgcatt cacgcttttc accgccggaa agcccagcac
    28741 atacatcgca gcatccagca tatgaatgcc gatgtcgatc agcgggccac caccctgcag
    28801 ttctttattg gtaaagacgc cccagccggg aacgccgcag cgacgcaggg cgcgggcggt
    28861 ggtaacgtaa atctctccca aaacgccgtt ggtcacctgt tcacgcagct gttgcgtatc
    28921 gagcgcaaaa cgatggtgaa agtcgtaggc cagcaccttg cccagtttgc gcgcggtatc
    28981 gcacatttcc cgcgcctgtt ctggcgtcat ggcgggcggt ttttcgcaca tcacatggca
    29041 gcccgcttcc agtgccatca gggtatgttc gtaatgaaaa cggttaggtg agcagacgct
    29101 aaccacatca ggtttcaccg ccagcagcat ggcctgcgga tcgtcccaca cggatgcatt
    29161 cccgtatttt tctgccagcg cctgcgcctg ggaaaggcgg ctgtcacaga cagccaccag
    29221 ttccagatcg ttgcgggtgc agtagtacga agcatgaacc ttatccgcca cctggcctgc
    29281 gcctattatc gcgacccgca gtggagagct tgtcattgca cttttcacaa agacctctta
    29341 gcaggtacgc aaccaggtca acgaatcacg gtacgcctgg gcaggatctt ctgcccggat
    29401 acgcccttca tacaccacat aaccctgata gttatcggcg cgcagctgtt caaacagcgc
    29461 gtggaaatcg agggtgccgc tgcccggctg gtaacgatgg ttatcggcaa tatgcacatg
    29521 accgagcagg tcgcggttgt cgtgcagcgc ctgcgccagg ttatcttctt cgatattcat
    29581 gtgatagaaa tcgccgataa tctgcacatg tttaagatcg ttttcgacga tgtaacggcg
    29641 ggcatcggcg agagtgttga tcatatgatc ctgatagcgg tttaacggtt cgagatacac
    29701 cacggttccg gtacgcgctg ccacttgttc cagcgtgcgc agggaatcgc tcaccatttt
    29761 acgatcgccg tccaggctgc gcggcgaggt catcggcggt aagcggaagg taaacatgcc
    29821 ccacgctgcc ggaacgacga tacctttacc ccccacttct gccagcgctt caagaatgcg
    29881 ttcgatctgc tttaagccat taagacgacg ctcttcgata aagtcaccaa tccagccgtc
    29941 atagccaccg caggcggtgg tcaccggtaa accggtttct ttgattgctg ctttgacttc
    30001 ttcgagattg ttgaccagca gtttgccgtc aatctcaaaa ccatcgaagc ccatctcttt
    30061 gatataacga aatttctcca gaatgttttc cggaaagaat gcctgattct gtgtgcctat
    30121 tttcattttt tcattccttt gaattaaaac gtaacgccca ttttgatgct ctgttccgga
    30181 tgctggtcga catactgcat gtagctttcc gggctattgg caaaggtcac caccgggtcg
    30241 attagatctt cacaattgag ataaccgttc atcagcagtt cccaacaggt ttcttcgata
    30301 cgcttgcggc tccagcgcgg ataatccggg ttcggttcgc tgcacgcgcg ggagaagaca
    30361 attttggcgt tattgaaatg ggcttcgcgt ccgaggttaa aaccttcggc aaacggtttg
    30421 gcaaacgcaa catacgagat ggtgccgcca taagccaggc cgcggagcgc cgattgcagc
    30481 gcgtcggcgt aaccgctggt ttcgataatc acatcggcac cctgcttgcc ggtcagcgtt
    30541 ttgatctctt tacctacgtc agtgccaatg gggttaaggc agaaatcagc accgtggcga
    30601 cgggcaatat cacagcgata ggcgatagga tcgacgccaa tcaccaccga cgcgccagcg
    30661 cgtttcgcca gttggatggc tatttgacca atcgcgccaa gccctaccac caccacaaaa
    30721 tcacccacgc gcacgttggc atcgcgcacg ccgctcatgg caaactgcgc cgggtcgtaa
    30781 cagacggcgt ttttccagga gctgccttgc ggcattttgc gcagcttgta gttattcacc
    30841 gcgttaatga tgaccgtctc ggagagtggg ccgtagccgc atacgctgtc gcccaccgcg
    30901 tagtcggtta cgtcgctgcc gcactcgata atgtcgccaa ccaccatgtt gccaagctgg
    30961 aatttgccaa actcaatgcc gcgcggcgca tctgccggac gcggagtgaa catctgccat
    31021 tcaccgttaa agtcttcatc gataaacggg ctggcggcgc agaagtcgac cacttccgtt
    31081 ccgtgtttcg gtgcgccgaa acgcacgcgg atcttaactt cattggctaa aatcgcccga
    31141 tcttcatact caaccagcgc agcaacgcgc ggtgctgtgg ctactaactt tttcatttaa
    31201 aactccttgt aaagaaactg gccgctttag cccttcacgc caccggcggt caaaccactc
    31261 ttaataaaac gttccgaaag cgcatacata atcaccaccg gcaatgcggt caccagtgag
    31321 gccgccatca tccgtcccca gatgtaatct ggcgtactga acagtgcgtt caggcccacc
    31381 ggtaaggtga aattgctggc gctggaaagg aaaatcgacg caaacagata gtcgttccac
    31441 gccaccataa agcagtagac gaacacggat atcagcccgg acatcgccag gggcacggtg
    31501 atacggaaga tgatttgcag acggttgagg ccatccatca tcgccgcttc ttcgatctca
    31561 tcagggatgg tgtcgaagta gcttttcagc ataaacacgg cggtgggcag ggtttgcgtc
    31621 accatggtaa tgatcagcgc catctcggtg tcgtaaatgc ccagcgcggt gatgattttg
    31681 aacagcggca ccaccagcaa aatgccagag aacatataca ccgtgtaaaa gctggcattg
    31741 atggtcatcc gacctttaaa gcgcaggcgg gaaagcgcgt aagccccaag aatgccgagg
    31801 aataccgcca ccacggatga aaccaccgac accaccagac tgttacggaa gtagtcgaca
    31861 aacgggaaaa tcatcgggtt aaaaatgtcg acgtaatgct ccagcgtcca ctgctgcggc
    31921 agcagcgtag gatgcagtga gatcgcctct ttcgcgccct tgaacgaggt catcagcatc
    31981 acaaaaaatg gaaacagcgt gatgatgaga aacagcgcca gcccgcagca aaaaccgatg
    32041 cgactgagtg tgcgtttatt tgttgccatt gaggttcacc cgtttcctgg tcagcagaat
    32101 gacagcgaaa ataatgatga agagcaccac cgagatcgcc gccgcttttc ctaaatcatt
    32161 aaaggcaaag gcggttttgt agagatatac accgagaata tcgaccttgg tcgtcagcaa
    32221 ataaacatcc gcgaacatgt agaacatcca gatggtgcgc agggtcacca ccgtcgccag
    32281 gaccggcata atcgcgggca gcgtgacgat acgaaaccgt tgccaggcat tagcgccatc
    32341 catctctgcc gcttcgtaca gcgatttgtc gatggtctgc aaaatcgcca gaaacgagat
    32401 aaaggcatac gggaagtagc gccagatggc gaacagcacc accagcacaa agctactgcc
    32461 cggattatcg aaccacagcg gtgcctgctc atagagatgc agaagatcga cgccgaggta
    32521 gttcacaatg ccgtagccgt tgttgaacat gtatttccag gcaaacacca gcgaaatgga
    32581 cggcgttacg taggagagga tcaccagcga acgcgcggtt ttgcgcagac ggaactcgcg
    32641 gttgaaaaac atcgccactg ccagcccgag cacggtgctg cccgccacca ccagcgcggt
    32701 ataccagacc gtcatccaca gcgagtgcca gaagccggga tcggagagga tacgcacata
    32761 gttactcacc ccaacaaagg ttgactcgat gttgggattg agcggcagac gtaaaaaact
    32821 gatttcgata ttcgacacca ttggccaggc caccagacca cccagcagta ataagctggg
    32881 cgcgagaagc agcagcgcaa agggcatatc ggaacgacct gaaaaaagcc tgttcattgc
    32941 ggattcctgt cctgggttta gtgctgttcg atcagttcat ccagcttttt ctggctcgct
    33001 tgcagcgtgc cagggagatc ggctttaccc acggtgacgt tatgcaccat ggagctcacc
    33061 acgccagacc ccgtcacatc acccatgcgg gtaaagtttt tatcccctac tgcaccaaag
    33121 acctgaatat ttggcagttc accgattagc tgattcggta gttcacccag cgccttaata
    33181 acgtcgttgt ctttccaggt ggcggtagtg acaaccgctt tattcaccgg cagcgcagca
    33241 cctggcgaca tcatcaccca gtcggcaatg ttgtctgcct gctccataaa ggtgacaaat
    33301 ttttctgctg cttccgtctc ttcggtcttt tgcccggcgg taatggtcag cgaggtcaac
    33361 atgccgtaga ccgcagagtt tttctcggtt ggcaccacga aaccgacgtt tttcgggtcg
    33421 ccttctttaa tcacagccgg aaggatatag gtggagtaaa tcgccatcgg cgcggtgccg
    33481 ttcataaagg cgtctttcac ttccatgatg tcgttagaac ccggcatagt gttggcagca
    33541 aggtcgcggt aataggtcag tgcctgcatc atctctggtg tatcaagggt gattttgcct
    33601 tcggcgttaa agacgttagc ctggttggat aacgcaaact gggagaagga ttgttccgtc
    33661 aacacgcttt ctgctgtagg cagcgcaatg ccgtattttt tattcgccgg gtcattcagt
    33721 ttctgtgcaa cgtccagcag ctgttgccag tttttcggct cctcaagccc cgcttttgcc
    33781 agcacatctt tgcgatacca gataccgcca atccaggcgc tgacaggaac accggtccat
    33841 gcgctaccat cttcggtacg cacaatacgc agtacgccat cgtaaaacgc gccttcacca
    33901 acgttgctga tgactgtggc aaccgctttg cgatcgataa gctgctcttt gtccatcact
    33961 ttggcgtagt catggctggt ttctatcact tccggcagcg aaccgctacg tgaaagagta
    34021 atgactttag tgttataggc atcttcttcc acgggcactt gcttaacggt gatgccaggg
    34081 ttttcttttt caaaacgggc aatcaattta ctgataacgg cctggcgctc ctgctccacc
    34141 gaagaatgca taaattcgat ggatacattc gttttatttt cttctttaca gcctgaaatc
    34201 agggcgcagg aaaccagtgc tgataacagc acgatttttg atttaatcat gagatgttcc
    34261 tttttattta atccacataa cctgccagag gcgcagcgtt atttcattac ccaatatatc
    34321 aacttcacta atcagatccc gaccatgcag attagtaata tttacatgct gaatattttt
    34381 actgacatta aacaatccag tcagacaatt accatcagcg ttacttcttt gaatacgcat
    34441 tacggatgaa ttaatcgtat cgatggtaaa attattatcc ggatgaaact cgttatggct
    34501 gcgacgaagt gtaattaaac gcgacaactc atgatatacc gcatgcctta atgtagcttc
    34561 atcgttcagt tctcgggtta tctctttact gtgatatttt ttacggttaa tcgcacggtt
    34621 atatccgagt ttttcgacac ctgcgtaatc attacgcgag ccaagaatac tttgaatata
    34681 tatcgctggc acaccgggga aacttaacaa aatcgcatgg gcgagaataa acctggcgca
    34741 acgttcttca tcgctactct cacggcggct taacgcatcc atataggtca catttatttc
    34801 atacggactg cgtgtaccgt cgggattatt tttccagttt actaatgcac cttcctgctg
    34861 taacgcctcg accagctcta atatttcgct ttcaggcaac aagccccgta gcgggtttag
    34921 cccgatgcca tcgtgagagg cgaggaagtt aaaccaggtg gtgttgctgg aaggtagtgt
    34981 caggttttgc gcccacgcac aaagcgcctc aacgttctgt ttttgcaccg catgcagcac
    35041 cagtggcggc agcgagaact ggtacaccat atgtgcttcg tcatcgcctg cgccaaagta
    35101 agcaatgttg tctttatgcg gaacattggt ctcggtaatg atcactgtac ctggcgcaac
    35161 gttatcaata atcgaccgta acagtttgat aatcagatgt gttttttcca gatggatgca
    35221 gctttttccc ggctctttcc acataaagcc aacggcatcc aggcggacat actcagcacc
    35281 tttcgcaagg taacagagta aaacatccac catcgccagc aacacttcag ggctacggta
    35341 attcaggtca atttgatcgt cactaaaggt ggtccataaa tggcgcgttg aatgatcgcg
    35401 catctggaat ggcgttaata acggtaacgc acgcgggcga gtgacggcgc tgagatcggt
    35461 ttgcgggtca acggcaataa aaaaatcttc aaaacctgga tgctgttgta aatagttttt
    35521 aaaccattca ctttttgccg acatatggtt gcagacaaaa tcaaacatta aatgactgca
    35581 ttcaccgagt tgctgaatat cctgccactc ccccgcttca ctggcgacct gatgataatc
    35641 aattaccgaa aagccatcat cagatgacca tggataaaac ggcaacaaat gaacatgtga
    35701 aaaaatgctt tgcagccatt gatggtaaaa ctgattaaat gtgggtaatg gttttaaatc
    35761 attgctgtga aattgatcgg cataggtaat taacacgaca tcactttcat cccagtgttt
    35821 tttacgtcgc tgtgtaatta atcgtttcgc actctcaaga cgcgttacaa gtttctgtaa
    35881 atgagttgcg gtaaatgttc caccgtagat ttcgtccagg taatccgtaa ttttctgttt
    35941 catgttgcct ttccgtggta gcggtcccat gaaacgagag actactccgc agaaaataaa
    36001 ctgtcaattg agcacaaggg ttttagtcac gcaggaaatc attttttacc agggaaaaag
    36061 cgtatttgtg agctgacgca aataatctat ggggatgtaa ataaggtgtg acagaaaaaa
    36121 aaacggcgca taagcgccgc tcatggtgaa ttcttttaac ctttgacctt cggcattgcg
    36181 atgtctttca ggccaccggc gttctcaacg tgggtatatc ccatctcgct aaggatctct
    36241 tttgcttgcc ctgactggcg tccggcactg caatacactt tcacggtgtc gtttttatcc
    36301 ggaacggcgg tggcaatgcg ctctttcact tccttcaggg gaatattgat ggccccctga
    36361 acgtgctctt gctgatactg ctctggaaca cgaacatcga tccagtgttc agcggcgaaa
    36421 acgggcagtg aaaacaccag tgccagagca agtaagcctt ttttaaacat gtaagcgtcc
    36481 ttctatggat gaaaattgcc atccattata cgcctgagat ttaacaattt ttgcgaaact
    36541 cccctcacct tttataacgc tgtgccagtt tattagcagc ccgacttaac agcggttcca
    36601 gcgccacagc cagcaacatt ttcagcggtc ggcgagcaac tgattttatc gcccagcccg
    36661 ctacacccgc cgggccatag cgcagtgcgg taagaagtac cagcttgcct gctaatttga
    36721 aaccaggctt taccttttgc ccggcctgtt gccagcgagt attcataact ttcctcacag
    36781 ttgacggaaa cggctacgta acgtgaaagt atcggaagtg acataacgtt ccatctcgcg
    36841 taaacgcgtt tcacttgccg ccagttcacg gtcgacttca tccagcaatt cgctgctgga
    36901 aggtagctgc tcaccaaagg ccatgttgtc cggcattgga tcaagcgcaa atgacaaaat
    36961 gatgtaagca accagggtaa acagcgccag accgaagaaa atcgacagca ccaccaggat
    37021 acgcaccagt tttgccggta catcaaaata gttggcaatc ccggcgcaga cgccgcggac
    37081 catgccctgc tgtggaatac gccataattt tttattgaga ttaatgcccg ccattagcga
    37141 tccctccagt tcggatgttc tgcatcaaga atagattcca gcgcctgaat acgttcgcgc
    37201 atccgttttg cttcatcagc cagttgcgct aatcgctgct gctcactttg cgacaattca
    37261 ctgcgaccag aacgattgct gtaatgcagc cataaccaga tcggtaaaac aaacagcaca
    37321 aaaatggtta acggaatagc cagaaatagc gcgctcataa gtactcctta gacgatgagc
    37381 ggcggcgcgt tcggacgccg cctggatgtt attattgatt gtcttgcttc attttggctt
    37441 ttaattgtgc cagttgttcg ctgattgcat catcggcttt cagttcggca aactgatcgt
    37501 ccagcgattt ttgtttaccg aagctgtggc tttctgcttc cgcttccatc tggtcaatac
    37561 gacgttcgaa agattcgaaa cgagccattg cttcatccag tttgccactg tccagctgac
    37621 gacgcacatc gcgcgacgag tttgccgcct gatgacgtaa catcaatgcc tgctggcgag
    37681 cgcgtgtttc gctcaatttg ttttccagct caccgatctc tttcttcatg cgtgccagcg
    37741 tgtcgtccac cagcgtcact tcatgttcca ggctcttaat cagatcggtc agtttctgtt
    37801 tttcaattaa cgctgcacgt gccagatcct ctctctcttt cagcagcgcc agttcggctt
    37861 tttcctgcca ttcaacttca cgcgccgacg cttgttcaat acggcgagtc agctgtttct
    37921 tttctgccaa cgcgcgcgcc gaagtagaac gtacttcaac cagtgtatct tccatctcct
    37981 ggatcatcag acgaaccagt ttctgtggat cttccgcttt ctctaacaga gcgttgatgt
    38041 tggcattcac gatgtcggca aagcgagaaa aaatacccat aatgttgtcc tcttgatttc
    38101 tgcgttcagg attgtcctgc tgaactgtta atacaatttg cgtgccaatt ttttatcttt
    38161 ttgatttata aagatctgat tgaagaatca acagcaacat gccaggatga gttagcgaat
    38221 tacactaaca agtggcgaat ttcatcatgg cagaatacaa agataattta cttggtgagg
    38281 cgaacagctt tctcgaagtg ctggaacagg tttcgcatct cgcaccgctg gacaaaccgg
    38341 tgctcatcat cggcgaacgc ggcaccggta aagagctgat tgccagccgc ctgcattatc
    38401 tctcctcccg ttggcaaggg ccgtttattt cccttaactg cgcggcgtta aatgaaaatc
    38461 tgctggattc cgaactgttt ggtcacgaag cgggggcgtt taccggtgcg caaaaacgtc
    38521 atcctgggag atttgaacgt gccgacggcg gtacgctatt tcttgatgaa ctcgctacgg
    38581 caccgatgat ggtgcaggag aaattattgc gcgtgattga gtacggtgaa ctggagcgcg
    38641 ttggcggcag ccagccattg caggtgaatg tgcggttggt atgcgcgacg aatgccgatc
    38701 tcccggcgat ggtcaatgaa ggcacttttc gcgctgacct gctcgaccga ctggcttttg
    38761 atgttgtaca actgccacca ctgcgcgagc gcgaaagcga cataatgttg atggcagaac
    38821 actttgccat ccagatgtgt cgggaaatca agctgcctct gttcccgggg tttacggagc
    38881 gcgccagaga aacattgctg aattatcgtt ggccgggaaa tattcgtgaa ttgaaaaacg
    38941 tggtggaacg ttcagtgtat cgccacggca ccagcgatta tccgcttgat gacatcatta
    39001 ttgatccctt taaacggcgt ccgcctgaag acgctatcgc cgtttcagaa accacctcgc
    39061 ttccaacact gccgctggat ttacgtgagt ttcagatgca gcaggaaaaa gagttgctgc
    39121 aactcagttt gcaacaaggg aaatataacc agaagcgcgc ggctgaatta ctggggttaa
    39181 cttatcatca gttccgcgcg ttgttgaaaa agcaccagat ttagcgaagt tgagcaatac
    39241 aacttgcagg atgcggcgtg aatgctttat tcggcataca taatcaatac gtcgtttgca
    39301 ggcctgataa gcgcagcgca tcagacatta ttgcgttggg ctattaatcg ctcagcgcat
    39361 cctgcaaaat tttcattgcc gcatcgaatt gcacatccgg gatggtcagc ggatacagga
    39421 agcgaatcac gttgccgtat gcgccacagg tcagcaggag cagcccctgc gccagcgcgc
    39481 gttgctggat tttctgtgca atcgccgctg acggcttgcc cgtttgcgga tcgttaaact
    39541 ctgccgcaat catcgacccc aggccgcgta ccgcagcaat ggccggaacg ctttctttgg
    39601 catcaatcaa cgtgtttttg agacgctggc ccagttgatt cgcgcgttcg cagagtgatt
    39661 ctttgtcgat aatattgagc accgcgtgcg cggcagccac cgccagcggg ttcccggcgt
    39721 aggtgccacc caacccgccc ggcgcgggcg cgtccataat attcgcgtta ccgaccacgc
    39781 ccgaaagcgg catcccgccc gcgaggcttt tcgccatcgt cattaagtcc ggcttatcgg
    39841 cgtaatgatc catggcaaac agtttaccgg tacgcgcaaa gccgctttgc acttcatcgg
    39901 caatcatcac aataccgtgc tcgtcgcaca ggcggcgaat ggcggcaacc agctcttttg
    39961 gcgcaacgtt gaaaccgccc tcgccctgca ccggttcgaa aataatggcc gcgacctgct
    40021 tcgcttcgat gtctgattta aacaagcgtt cgatggcgtc gagggagtcc tgtgttgaaa
    40081 tgccgtgtaa atctgacgga taaggtacgt gatacaccga accagggaac gggccgaagc
    40141 cgattttgta cggcgcaact tttccggtca gcgccatggt catatatgta cgaccgtgga
    40201 agccaccgct aaacgcaatc acgccaggtc gtccggtatg ggcgcgagca attttcaccg
    40261 cgttttccac cgcttccgca ccggtagtga agaatgcggt tttagcctgc ccgctcactg
    40321 gggcaagggc gttaattttc tccgccagcg tgacgtagct ttcgtacggc acaatctgat
    40381 acgccgtgtg ggtaaactgt tgcaattgct gctccaccgc cgcgaccaga tcagggtggc
    40441 gatgcccggt attcagcacc gcaatgcctg cggcgaaatc gatgtattcg ttgccctcta
    40501 cgtccttcag cgtagcgttt tctgccgact gggcgaagaa gttacacatc acgccaaccc
    40561 cgcgtggggt agcagaaaga cgacgctgat ggaattcatt gttgctcatg tttcacacct
    40621 tttgactctt cactgttaat cggaaattaa acgcccagac gatcgcgcag gctgtaataa
    40681 gccgcgccca tcgcggtaaa tggcacgcgc agcgtgcgcc cgccggggaa cgggtaatgc
    40741 ggcagattgg cgaaggcatc gaaacgttcg gcgtcgccgc gcagcagttc ggcaatcaga
    40801 cgcccggcaa gatgggtaca ggtgacgccg tggccgctgt agccctgcat gtaatagatg
    40861 ttggtatcaa ggcgaccgaa ctgcggcatg cgcgacaagg tcagcaggaa gttgcccgtc
    40921 cagcggtaat caattttcac gcccttcagc tgcgggaagg ttttcagcag tttcggcacc
    40981 acaaggcgct caacgtcatc cgggtcgcgc gcgccgtaga ccacgccgcc gccgtacagc
    41041 aggcggttgt cggcggtaag acggtagtaa tcaagcagat agttacagtc ttccacacag
    41101 tagtttttcg ggatcagcga acgggctaaa tcttccgaca gccgttcggt ggtgatcacc
    41161 tgcgtgccgc acggcatgct gcgtttcgcc agttccggct ctactttatc gcccagatac
    41221 gcattcccgg cgacaatcac atacttcgcc gtcacctgac ctttggcagt tcgcacaacg
    41281 gctggcgtgg tgtgctggat ctgcgtcacg gcagaaagtt catacacgcg cccgccgttg
    41341 aggcggatgg cgtccgcttc gccaatggcg aggtttagcg gatgaatatg cccaccgctg
    41401 tgatccagca gcgcaccggt atagcgatcg ctggctactt cacggcgaat ggcgttggcg
    41461 tccagcaatt ccagctgttt attgccgtag cgttcccagt tctctttctg ctcttcaagt
    41521 gtggcgagct gtttatcatt catcgccaca aacaggccgc cggggcggta gtcgcagtca
    41581 atctgataac gtttgatacg ttcgcggatg atctcaccac cttcgaacat catgctgccg
    41641 agcatacggg cggtgtccat gccgtagctt ttttcgatca cgtcgatgtc gcggctgtag
    41701 gagttcacaa gctgcccgcc attgcgcccg cttgcgccaa agccgatgcg tgaggcttcg
    41761 agaaccacta cgtcaaagcc cgcttccgcc agatgcagcg cggaagagag cccggtatag
    41821 ccgccgccga ccacgcaaac gtcgcaggaa atcgactcat tcagcgtgtc gaatggcgcg
    41881 tatttattcg cactggcggc gtagtaactg ctggtatgtt cggtcatttc aggcctccag
    41941 gcttatccag atggttttca gttcggtgaa tttttcaagg gcatgcaggg atttgtcgcg
    42001 accgttgccg ctctgcttat agccgccaaa cggcacggtc atatcgccgt cgttgtagtt
    42061 attgacgaag acggaaccag ccttcaggcg gcggctcatg cggtgcgcgc gggagaggtc
    42121 gcgcgtccat accgccgcgc caaggccgta ctggctgtcg ttggcaagct gtagcgcctg
    42181 gtcttctgat gtgaaacgcg tgaccaccag caccggaccg aaaatctctt cgcggcttaa
    42241 ggacgcattc gggtctacat ccacaaagat ggtcgggccg atggcggcag ccagctcagc
    42301 gttacgacca tccagcagca gttgcccttt gctttcgcct tcccgaataa agctatggac
    42361 cgagtcggcg tgggcgcagt cgattaaggt gcccatggtg gttgcgggat caagtggatg
    42421 gcccggctgc cagttttgcg cctgctgttt taacagggct aagaattcat cggcgatgct
    42481 ctcttccagc aacaaacgcg ttccggcgat gcacacctgg ccctggttgt agaagatgcc
    42541 tgcggcggtg gcgcttgccg ctttttgcag atccggacaa tcggcgaaaa cgaggttggc
    42601 gcttttgccg cccgcttcca gccagacgcg tttcatgttg ctgtcgcccg catctttcag
    42661 cagctgtttc ccggtacggg ttgaaccggt aaaggcaatg gcgtcgatat cgttatgacg
    42721 cgacagcgcc tgccccgctt catgaccaaa acccgtcacc acgttcaaca caccatccgg
    42781 caagcctgct tctttcgcca gccccgcgag acgaatcgca ctgagcggtg atttttcaga
    42841 cggttttaga atcacgctgt ttcccgccgc cagcgccggg ccgagtttcc agcaagtcag
    42901 caacagcggg aagttccacg gcacgatggc ggcaatcacg ccgaccggtt cacgcacgat
    42961 catcgccagc tcatggctac tggtggtcgc cacttcgcca tacactttgt cgatcgcttc
    43021 ggcgtaccag cgaatggcgc gcgccgcgcc gggaatatca tcacgcagac tgtgacgaat
    43081 cggtttgccg gtgtcgagag tttccagcag tgccagctct tcggcgtggg cttccattaa
    43141 atcggcgagt ttattcagta ccgctttacg tttagccgga gaagacagtg accagtcgcc
    43201 gcgttcaaat acgccgcgtg ctgcgctcac cgcacggtcg atatcgacgc tcttgccgcg
    43261 ggcaattttc gccagcggtg cctgggtgac cggatcaacg gtttcaaagg tttcattttc
    43321 cgccgcagca gtatattcac cgttaataaa taagcggttt tcaatggcga gacttaacgc
    43381 tttatcctgc cagtaagcca gatgatgaaa attcattatg actcctgttt cacgtctatc
    43441 agatatatgc agtttgtgaa cgtcagggcg cagagatccc cgggccgtat gcccgtttat
    43501 ttctggcgac attctttata tacacttcat ccttcaagcc gcctctgcct tggctgcgtt
    43561 cgctcccccg aatcacttac ctgagtaagc tcatcgggat tcgctcactt gccgccttga
    43621 tgcaacttga atgattttgt gtatagattg ccgggcgcat tgcgcttacc cggcttacag
    43681 aacaaaaaga ttaaaacgtg gtgggcgtat gggcgctgat aattcggcaa atacctgccg
    43741 acgtattact gaaactgtgc ggtatgccgg tattaatggc ataactttgc cccgcgacga
    43801 ggtggtaatc ctgaccatta atcgtcagaa caatttcacc ttccagtact gtgcctattt
    43861 cctcaccctg atgcttaatt ctttccccag tggttgtgcc cggctggtac gtttcaaaga
    43921 tcatcgccag cgtgcgattc gggttaccgt tatgaaccag cttcattgac acaccctgac
    43981 tgcccatctc aattaagtcg tcctgattaa tgacgacctg cggctcatca ggtttttccg
    44041 gctcggaaaa gaattccgag agtgacagac catacacctt cagcagcttt tgcagcgtac
    44101 tgatggcagg gctgacttta tcttgttcta tcgtactgat agcactgtga gtcagcccgg
    44161 agagttcggc ggcacgacgt tgtgaaagcc cctgctgctg gcggatttcc gacaagcgtt
    44221 ttcctggcgc cagtccctca tcactcatat ttgcatttcc ttaaactgta gtggtcagag
    44281 tcgctgtttt tcagcgatat ggtgctgaca agcggtgata aagccctcga acaatatacg
    44341 cgaaagcgcg tactcgctac tgttccattc cgggtgccac tgtacgccca gcgcaaaagg
    44401 atgattgatg acgctaaccg cctcaactaa gccatccggc gagcgagctt caacacgcaa
    44461 ccgtgggcta acgaccttcg ccccttgccc atgcagagag tttacccaaa agttgctaca
    44521 ttcaggtaac aacgcagaca gtaatccccc ctcttcaacc tgaacttcat gcgacggtgc
    44581 atattgctgt tccaccggca gttcgggatc ttcccgatgt tccagcaatt caggctgttc
    44641 gcacagcttg cgatgcaacg aaccaccggt tgccaccacc agttcctgta aaccccggca
    44701 gatggcgaaa atggggatgc gcctttcgag tgcggcatta attaacgcca tgctcagaag
    44761 atcacgcccg ggatcggcgt caggctcatc gccgttttca ccatataggt gcggctgcac
    44821 attgctggga ctaccaggaa gataaatgcc atcgagtttc ggcaaaagtt gttcaagtaa
    44881 tgacggttcc gccagcgcat gtggtagcgc aattggcaag ccgcctgcgt ggatgatggc
    44941 attcaggtac ttttcttgca gagtctgggt cgcatgaccc ttaagcctgt tcctgcacat
    45001 tacgacaccg ataaccggat tgttcattat attttccatg ctcaatctca caaagtggac
    45061 taaattatcg ccattactgc ggtttaaatg acctttatgt tcaatatttt ttcaatctag
    45121 cagtggtttt tcatttttgc aaaccaaatt taacatttga caaacattta gtttgcatac
    45181 agattcgaat ggtggtcatt atattttacg ctttgataac gagcggaaaa caaaccaaag
    45241 gcgaagaatc atggaaacca atatcgttga agtagagaac tttgttcagc agtcagaaga
    45301 gaggcggggt agcgccttta cgcaggaagt gaaacgctac ctagagcgct acccgaatac
    45361 gcaatatgtt gatgttttac ttactgattt gaatggttgc ttccgtggaa aacgtatccc
    45421 tgtttcaagc ctgaagaagt tggagaaagg gtgttatttc ccggcctcgg tgtttgcaat
    45481 ggatattctg ggcaacgtag tagaagaagc gggtctgggt caggaaatgg gcgagccgga
    45541 tcgtacttgt gttcctgttc tcggttcctt aaccccttct gccgccgatc cagagtttat
    45601 cggtcagatg ctcctgacca tggtcgatga agatggcgct ccctttgacg ctgagccgcg
    45661 gaacgttctt aaccgcctgt ggcagcagct acgccagcgc ggattgttcc cggttgtagc
    45721 ggtagagctg gagttctatt tactggatcg tcagcgcgac gctgaagggt atctgcaacc
    45781 gccctgcgcg cccggcaccg atgaccgcaa tacacaaagc caggtttact ccgttgataa
    45841 cctcaaccac ttcgccgatg tgctcaatga tattgatgaa ctggcgcagt tacagttaat
    45901 tcccgccgac ggtgcggtcg ccgaagcctc gccgggtcag tttgaaatca acctttacca
    45961 taccgataac gtgctggaag cctgcgatga tgcgctggcg ctaaaacgcc tggtgcgtct
    46021 gatggcagaa aagcataaga tgcacgccac ttttatggcg aagccgtatg aagagcacgc
    46081 gggcagcgga atgcatatcc atatcagtat gcaaaataac cgtggcgaga acgtgctttc
    46141 tgacgcggaa ggcgaagatt cgccgctgct gaaaaagatg ctcgccggga tgattgacct
    46201 gatgccgtca tcaatggcgt tgctggcacc aaacgtgaac tcgtatcgcc gcttccagcc
    46261 gggaatgtat gtgccgacgc aggcgtcgtg gggccataac aaccgcaccg tcgccctgcg
    46321 tattccgtgc ggcgaccgtc ataatcaccg cgtggaatat cgcgtggcgg gtgccgatgc
    46381 caacccatat ctggtgatgg cagcgatttt tgccggtatt ttgcatggcc ttgataacga
    46441 gctgccgttg caggaagaag tcgaaggcaa cgggctggaa caggaaggct tacccttccc
    46501 gattcgccag agcgatgccc tgggtgagtt tatcgagaat gatcacctgc gccgctattt
    46561 aggcgaacgg ttttgccatg tgtatcacgc ctgtaaaaat gatgagctgt tgcagtttga
    46621 gcgcctcatc acagaaacag aaattgagtg gatgttgaaa aacgcgtaat tccccatgcg
    46681 gggttgtttc atcggcctga gcggagccag gcctgacatt tgctggtaat ccagacattt
    46741 cccggatgtc gcgttatgga gcgcaggcgg caacgggctt ccgttaacga ttaagactgt
    46801 gacgaggaaa tgagatgatg ccgatgatta aatccccgca tggcgagggt ggctgcgtat
    46861 gcgcccctcc tgctactgac tggactccgc cccctttgct tcccttgcta aacaggtttg
    46921 atttcaggtt gacacgaccg caaaccttat tacgcagggg aggcagcaat tatggctatt
    46981 aattcaccac tgaatattgc tgcgcaaccc ggcaaaaccc gtctgcgaaa atcactgaaa
    47041 ttgtggcagg tggtaatgat gggtctggcc tatctcacgc cgatgaccgt atttgatact
    47101 tttggtattg tctccggcat tagcgacggt cacgttccgg cgtcctattt gctggcgctg
    47161 gcgggcgtgc tgtttaccgc tatcagctac ggcaaactgg ttcgccagtt tccggaggcc
    47221 ggttcggcct atacctacgc gcaaaagtcg attaatccgc acgtcggatt tatggtcggc
    47281 tggtcatcac tgctggatta tctctttttg ccaatgatca acgttttgtt ggcgaaaatc
    47341 tatctctccg ccctcttccc ggaagtgccg ccgtgggtgt gggtggtaac cttcgtcgcc
    47401 attttaaccg ccgcgaatct gaagagcgtc aacctggtcg ctaacttcaa taccctgttt
    47461 gtactggtgc aaatctccat catggtggtg tttatcttcc tggtggttca gggactgcat
    47521 aaaggagaag gcgttggcac cgtctggtca cttcagccgt ttatcagcga gaacgcgcac
    47581 ctgatcccga ttattaccgg tgcgacgatt gtctgtttct cgttcctcgg tttcgatgcg
    47641 gtgaccacgc tttcggaaga gacgccggac gccgcacgcg tgatcccgaa agccatcttc
    47701 ctgacggcgg tatacggcgg cgttatcttt atcgcggcgt cgttctttat gcagctgttc
    47761 tttcccgata tcagccgctt taaagacccg gacgccgcac tgcctgaaat tgcgctgtac
    47821 gtcggcggta agctgttcca gtcgattttc ctttgcacca cgtttgtgaa cacgttagcg
    47881 tctggcctgg cctcacatgc cagcgtgtca cgtctgctgt atgtgatggg gcgcgacaat
    47941 gtgtttccgg agcgcgtgtt tggctatgtg catccgaaat ggcggactcc ggcactgaac
    48001 gtcattatgg tcgggattgt cgcgatgtcg gcgctgttct tcgatttagt taccgccaca
    48061 gcgttaatta acttcggcgc gttggtagcg tttaccttcg ttaacctgtc ggtgttcaac
    48121 cacttctggc ggcgcaaagg aatgaataaa agctggaagg aacacttcca ctatttatta
    48181 atgccgctgg ttggcgcgct gacggtgggc gtgctgtgga ttaacctcga agccacctcg
    48241 ctgacgctgg gtctggtatg ggcttcgtta ggcggcgcat atttgtggta tctgatccgc
    48301 cgctatcgca aagtgccgct gtacgaaggt gacagaacgc cagtgagcga aacgtaatag
    48361 tcggatgcgc gccgcattat cctgcctgag ggtaatgcgc cgcgccatta actataaaaa
    48421 gatgtatgac ccggatcacg agtgctatca tccgccctta tgcttagcgc attaacccca
    48481 ggggcaaata atgaaccacg atatcccgct aaaatatttt gatattgccg atgagtacgc
    48541 gaccgaatgc gcagagcctg tcgcagacgc tgaacgcaca ccgctggccc actacttcca
    48601 gttactgctc acccgtttga tgaacaatga agagatcagc gaagaagccc aacatgaaat
    48661 ggctgctgaa gcggggatta atcccgtacg cattgatgag atcgcagagt ttttgaatca
    48721 atggggtaat gagtaattat ttaattgcag acgatcggtc agtatcgttg tccggataaa
    48781 gcgttcacgc cgcatccggc taacaatgac acgcactgca gcgtgcttta atgggtttct
    48841 cacgatcgta ccgctctgct cctcgctaag ttgttctgtg agttatagtt atagagaaat
    48901 cacgggtacg aatgttgaac agatggcgac gccacctgtt caacgaccaa ccacacggcc
    48961 ctcattacga cccctgaaca caaacggtaa tggcaaatgt cattaaatat tagtaagata
    49021 gcctctttac ataaaatccc ttaatattat gcgccaggta ttatcgtctc ttttggtgat
    49081 tgctggactt gtgagtggtc aggcaatcgc cgcgcctgaa tcccccccgc atgctgatat
    49141 ccgcgacagc ggttttgtct attgcgtcag cgggcaagtc aacaccttta acccatccaa
    49201 agcgagcagt gggttaattg tcgataccct tgccgcccag ttttatgatc gactgctgga
    49261 tgtcgatccc tatacctatc gcctgatgcc ggaacttgcc gaaagctggg aagtactcga
    49321 caacggcgcg acctatcgct tccacctgcg tcgcgatgtt ccgtttcaaa aaaccgactg
    49381 gtttactccc actcgtaaaa tgaatgccga cgatgtggtg tttaccttcc agcgaatttt
    49441 tgaccgcaac aacccgtggc ataacgtcaa cggcagcaac ttcccctact tcgacagcct
    49501 gcaatttgcc gataacgtga aaagcgtccg caaactggat aatcataccg ttgagttccg
    49561 actcgcccag ccggatgctt cttttttgtg gcacctcgca acccattatg cttcggtcat
    49621 gtcggcagaa tatgcccgga agttagagaa agaagatcgc caggagcaac tcgaccgtca
    49681 accggtcggc accggtccgt atcagttgtc ggaataccgc gccgggcaat ttattcgcct
    49741 acaacgtcat gatgacttct ggcgcggtaa accgttaatg ccgcaggtag tggtggattt
    49801 aggctccggc ggcaccggac gtctgtcgaa actcctgacc ggggaatgcg acgttctggc
    49861 ctggcctgct gccagccagc tatccatttt gcgtgacgac ccgcgcttgc gtttaacgct
    49921 gcgtcctggg atgaacgtcg cctatctggc atttaacacc gccaaaccgc cgctaaataa
    49981 tcccgctgtc cgccatgcac tggcactggc gattaataac cagcgcctga tgcaatccat
    50041 ctattatggt acggctgaaa cggcggcctc tattttaccg cgcgcctcgt gggcctatga
    50101 caacgaggct aaaattactg aatacaatcc ggcgaaatcg cgcgaacagt tgaaggcgtt
    50161 ggggctggaa aacttaacgc tgaaactgtg ggtgcccaca cgttcgcagg cgtggaaccc
    50221 cagtccactg aaaactgccg aactgattca ggcggatatg gcgcaggttg gcgtaaaagt
    50281 ggtgattgtg ccggtagaag gtcgctttca ggaggcgcgg ttgatggata tgagccatga
    50341 tctgacgtta tccggttggg cgacggacag taacgacccg gacagtttct tccgtccgtt
    50401 actgagctgc gcggcaattc attcacagac caacctcgcc cactggtgcg atccgaaatt
    50461 cgacagcgtg ttgcgtaagg cgctctcctc gcagcagctg gcagcgcgta ttgaagccta
    50521 tgacgaagcg cagagtattc tggcgcagga attacctatt ctgccgctgg cgtcgtcatt
    50581 gcgtttgcag gcctatcggt acgatatcaa aggtctggta cttagcccgt ttggtaacgc
    50641 ctcctttgct ggggtgtatc gcgagaaaca ggatgaggtg aaaaaaccat gattatcttc
    50701 accttacgcc gcattttgtt attgattgtc accttgttcc tgctgacctt cgttggcttc
    50761 agtttgagct atttcactcc acacgcgccg ttacaaggcg cgtcactgtg gaatgcctgg
    50821 gtgttctggt ttaacggcct gatccactgg gattttggtg tttccagcat taacggtcaa
    50881 ccgattgccg aacagttaaa agaggtcttc cctgcgacga tggaactgtg catcctcgcc
    50941 ttcggttttg cgctgattgt tggcatcccc gtggggatga ttgccgggat tacgcgccat
    51001 aagtggcagg ataacttgat caacgccatc gccttgctgg gcttttcaat cccggtgttc
    51061 tggctggcgc ttctgttgac gctgttttgt tcactcacgc taggttggtt gcccgtttca
    51121 gggcgtttcg atctgctgta cgaagtgaaa ccgattaccg ggtttgcgtt gattgatgcc
    51181 tggctttcgg actcaccatg gcgggatgaa atgatcatga gcgcaatccg ccatatgata
    51241 ttgcccgtga tcaccctgtc ggttgcgcca acaactgaag tgatccgact gatgcgtatc
    51301 agcactatcg aagtgtatga ccagaactat gttaaagcgg cggctacccg cggtttgtcg
    51361 cgctttacta ttttgcgtcg ccacgttctg cataacgcgt taccgccggt tattcctcgc
    51421 ctgggcttac agttttccac catgctgacg ctggcgatga tcaccgaaat ggtttttagc
    51481 tggccgggtt tagggcgctg gttaattaac gccatccgcc agcaggatta cgcagccatt
    51541 tccgccggag tgatggtgtg tggctcactg gtcattattg ttaacgtgat ttctgatatt
    51601 ttgggtgcca tggctaaccc tctgaaacat aaggaatggt atgccttacg atagcgtata
    51661 cagcgaaaag cgcccgccgg gcacgctgcg taccgcctgg cgcaaatttt atagtgacgc
    51721 ctctgcgatg gtcggcctgt acggctgcgc ggggctggct gtactgtgta tttttggcgg
    51781 ctggtttgcg ccttacggca tcgaccagca atttctcggt taccaattat tgccgccgtc
    51841 atggtcgcgc tatggcgaag tttctttctt cctggggact gacgacctcg gacgcgatgt
    51901 tttaagccgt ttactgagcg gagctgcgcc aaccgtgggt ggcgcatttg tggtgacgct
    51961 tgccgcgacg atctgtggcc tggtactggg caccttcgcc ggggcgacgc acggcctacg
    52021 ctcagcggtg cttaaccata ttctggatac cttactggcg atcccttcgc tgctgctggc
    52081 aattatcgtt gtagcgtttg ccggaccgag tttgtcccac gctatgtttg ctgtctggct
    52141 ggcattgctg ccgcgtatgg tacgttcgat ttacagcatg gtgcatgacg aactggaaaa
    52201 agagtacgtt atcgccgccc gtctggatgg cgcatcaacg ctgaatattc tctggtttgc
    52261 tgtgatgcca aacatcaccg ccgggctggt caccgagatc acccgcgcac tgtcgatggc
    52321 aattctcgat atcgccgcgc tgggctttct cgatctcggc gcacaactcc cctcgcctga
    52381 atggggagcg atgctcggtg atgcgctgga actgatttat gtcgccccgt ggactgtcat
    52441 gctgccaggt gcggcaatta tgattagcgt gttgttggtt aacctccttg gcgatggcgt
    52501 tcgtcgtgcg attattgcgg gggtggaata atgccattac tcgatattcg taacctgacc
    52561 attgaattta aaaccggtga tgagtgggtt aaagccgtcg accgggtaag tatgacctta
    52621 accgaaggtg aaatccgcgg tcttgttggt gaatccggtt ccggcaagag tttgattgcg
    52681 aaagcgattt gtggggtgaa taaagataac tggcgtgtta ctgctgaccg tatgcgtttt
    52741 gatgatatcg atttgctgcg tctctccgca cgcgaacggc gcaaactggt tggccataac
    52801 gtgtcgatga ttttccagga accgcagtcg tgtcttgacc cttcagaacg tgtgggtcgc
    52861 cagttgatgc aaaacatccc agcctggacc tataaaggcc gttggtggca gcgttttggc
    52921 tggcgcaaac gccgtgccat tgaactgctg caccgcgtgg ggattaaaga tcacaaagat
    52981 gcgatgcgca gttttcccta tgagttgacc gaaggtgaat gtcagaaagt gatgatagcc
    53041 attgcactgg cgaatcaacc gcgtctgctg attgctgacg aaccgaccaa ctcaatggag
    53101 ccaacaaccc aggcgcaaat ctttcgcctg ctgacgcgtc tcaaccaaaa cagcaatacc
    53161 actattttgc ttatcagcca tgacttacaa atgcttagcc aatgggcgga taaaattaac
    53221 gtgctttact gcggtcaaac agtggaaacc gcgccgagta aggagttggt gacgatgcca
    53281 catcatcctt atacccaggc gctgatccgc gcgataccag acttcggcag cgcgatgccg
    53341 cataaaagtc gcctcaatac gctgcccggc gctatcccac tgctggaaca gttaccgatt
    53401 ggttgtcgtc tggggccacg ttgcccgtat gcacaacgag aatgcattgt gacgccacgt
    53461 ttgacggggg cgaaaaatca tctctatgcc tgtcatttcc cgctgaacat ggagaaagag
    53521 tgagatgatc gaaacgctgc ttgaagtgcg taatttaagt aaaacgttcc gctaccggac
    53581 cggctggttt cgtcgtcaga ccgtagaagc ggtaaaaccc ttgagcttta cgctacgtga
    53641 aggccagaca ctggcgatta ttggcgagaa tggttcgggt aaatccacgc tggcaaaaat
    53701 gctggcggga atgatagagc ctaccagcgg cgagttattg attgacgatc atccactgca
    53761 ttttggcgat tattccttcc gcagtcagcg tattcgcatg atttttcagg atccttcgac
    53821 ctcgttgaat ccccgtcaac gtatctcgca aattctggat tttccactgc gcctgaacac
    53881 cgacctggaa ccggaacagc gtcgcaaaca gattattgaa acgatgcgta tggttgggct
    53941 gctaccggat cacgtcagtt actatccgca tatgttggca cccggacaaa aacagcgtct
    54001 gggtctggcg cgcgcgctga tattgcgccc aaaagtcatt attgccgatg aagccctcgc
    54061 ttcactggat atgtcgatgc gttcgcagtt gattaatctg atgctggagt tacaggaaaa
    54121 acagggcatt tcgtatattt atgttaccca gcatatcgga atgatgaagc acattagcga
    54181 ccaggtgctg gtgatgcatc agggcgaggt tgtcgagcga ggcagcaccg cggatgtgct
    54241 ggcatcaccg ctgcatgaac tcaccaaacg gctgattgcc gggcactttg gcgaagcatt
    54301 gacagcggat gcgtggcgta aagatcgcta attttctgaa tcctccttcc agattattca
    54361 cctcattctg acaagagatt aaatcttgtc agaatgaggt gatggataaa ataaaatcaa
    54421 atgcgcgtga tttaagaagc aatctcactt tgcaggaacg aaagctctgg cgatatcttc
    54481 gcagccgacg ttttggtgat ttcaaatttc gccgtcaaca tccggtgggg agctacattc
    54541 tcgattttgc ttgctgctcg gcgcgcgtag tcgttgagct ggatggtggg cagcatgatt
    54601 tggcagttgc ctatgatacc aggcgcacta gctggcttga gtcgcagggc tggaccgtgc
    54661 tgcgtttctg gcataacgag attgattgta atgaggaggc ggtgctggag attattctgc
    54721 aggaactgaa ccgccgatca ccctcaccct gaaagggcga gggggccgac cgagccgaat
    54781 agctgttgtg gtgaaaacat ggagacagtg ctggagaata ttcggcaagg tctgaaccat
    54841 cccagccatc gccataaaag ggttaggggc tgtatgagcc tgtttgttgc tggggtaaca
    54901 atatttgcac aatatggtcc cctcgcccct ctggggagag ggttagggta aggggaaagg
    54961 cgcccaccct gccgcagcct gctccggtcg gacctggcaa ctatagctac tcacagccag
    55021 gttgattata ataaccgttt atctgttcgt actgtttact aaaacgacga atcgcctgat
    55081 tttcaggcac aacaagcatc aacaataagg attaaagcta tgggttttct ttccggtaag
    55141 cgcattctgg taaccggtgt tgccagcaaa ctatccatcg cctacggtat cgctcaggcg
    55201 atgcaccgcg aaggagctga actggcattc acctaccaga acgacaaact gaaaggccgc
    55261 gtagaagaat ttgccgctca attgggttct gacatcgttc tgcagtgcga tgttgcagaa
    55321 gatgccagca tcgacaccat gttcgctgaa ctggggaaag tttggccgaa atttgacggt
    55381 ttcgtacact ctattggttt tgcacctggc gatcagctgg atggtgacta tgttaacgcc
    55441 gttacccgtg aaggcttcaa aattgcccac gacatcagct cctacagctt cgttgcaatg
    55501 gcaaaagctt gccgctccat gctgaatccg ggttctgccc tgctgaccct ttcctacctt
    55561 ggcgctgagc gcgctatccc gaactacaac gttatgggtc tggcaaaagc gtctctggaa
    55621 gcgaacgtgc gctatatggc gaacgcgatg ggtccggaag gtgtgcgtgt taacgccatc
    55681 tctgctggtc cgatccgtac tctggcggcc tccggtatca aagacttccg caaaatgctg
    55741 gctcattgcg aagccgttac cccgattcgc cgtaccgtta ctattgaaga tgtgggtaac
    55801 tccgcggcat tcctgtgctc cgatctctct gccggtatct ccggtgaagt ggtccacgtt
    55861 gacggcggtt tcagcatcgc tgcaatgaac gaactcgaat tgaaataatc attctgttgg
    55921 taaatattgg gcggcgttct gccgcccgtt atctctgtta tacctttctg atatttgtta
    55981 tcgccgatcc gtctttctcc ccttcccgcc ttgcgtcagg ataacgattt cctttacgac
    56041 caaggagcgc ccatggaaca acgccacatc accggcaaaa gccactggta tcatgaaacg
    56101 caatccagta ctgcggagta tgacgttctg cctctggtcc cggaagccgc aaaggtcagc
    56161 gatccctttc tgctcgacgt gatccttgat gaagaaacgc tggctccctt cctttcatgg
    56221 ctggtccctg cgcgcgttct tgcagtggaa ttgttccctg accagcttac cgtaacccgt
    56281 tcacagacct tcaccgctta tgaacgcttg tctacggccc tgacggttgc tcaggtttgc
    56341 ggcgtccagc ggttatgtaa ctactattcg gcgcgactta cgccgctccc cgggcctgat
    56401 tcctccaggg aaagcaatca taggttggca caaatcacgc aatatgcccg ccaactggct
    56461 agctcgcctt ctattatcga caaccgatcg cgccagcatc tgaatgacgt cggtcttact
    56521 gccggggact gtgtgatcat taaccaaatc attggtttta ttggctttca ggcgcggacc
    56581 attgcgacat ttcaggctta tctcgggcac ccggtacgct ggttacccgg tctggaaata
    56641 caaaactacg ccgacgcgtc actgtttgct gatgaatcaa tacgctggcg aagcagctat
    56701 gaggtggaaa aactacctga agagtacaca aaaagttcaa cagcagaact ttgccaactg
    56761 gccgaaacac tctctctcca ccctatttca ctttcccttc tcgaaaggtt gttaaacagt
    56821 acacgggtta atacacagcc ggataataag cttgcggcgt tgttatgcgc gcggataaat
    56881 ggcagtcctg cttgttttgc cgcctgtatg gattcatcaa atgaatataa aaaaatcagc
    56941 ccccttctgc gcaagggcga aaatgaaatt aaccaatggg ctgaccgtca ttctgttgag
    57001 cacgctaccg ttcaggcgat acaatggctg acccgagcac ccgatcgctt tagcgccgcc
    57061 cagttcagcc ctttactcga acacgaaaaa tcatcaacgc agattattaa tctgctggta
    57121 tggagcgggc tgtgtggctg gataaatcgc ttaaaaatcg cgttgggtga gacatattaa
    57181 ccttgccgcg tcagacagat tcgcgtaaaa ctgtcagccg ctctaatggc caccaaaata
    57241 gacaattatg tttcaggaca acccgctgct agcgcagctt aaacagcaac tgcattccca
    57301 gacgccacgc gctgaagggg tggtaaaagc cacagaaaaa ggctttggct tcctggaagt
    57361 cgacgcgcaa aaaagttatt tcattccgcc gccgcagatg aaaaaagtca tgcatggcga
    57421 ccgaattatc gcggtgatcc acagtgaaaa agaacgtgaa tccgcagagc cagaagaact
    57481 ggttgaaccg ttcctgactc gtttcgtggg taaggttcag ggcaaaaatg accgtctggc
    57541 catcgttcct gatcatccac tcttaaaaga cgccattcct tgccgcgcag cccgtggcct
    57601 gaaccacgag tttaaagaag gcgactgggc ggttgccgaa atgcgccgtc atccgctgaa
    57661 aggcgatcgt tctttctatg cagaactgac acaatacatc acttttggtg acgaccactt
    57721 tgtaccgtgg tgggttaccc ttgcacgcca taatctggaa aaagaagcac cagacggcgt
    57781 cgctaccgaa atgctcgatg aaggtctggt tcgtgaagat ctgaccgcgc tggattttgt
    57841 caccatcgac agtgccagca cagaagatat ggatgacgcc cttttcgcta aggcgttgcc
    57901 ggatgacaaa cttcagctga ttgtggcgat tgccgatcca accgcgtgga ttgctgaagg
    57961 cagtaagctg gacaaagccg cgaaaattcg cgcattcacc aactatctgc ctggcttcaa
    58021 catccctatg ctgcctcgcg agctttctga cgatctctgc tcactgcgcg ccaatgaagt
    58081 ccgcccggta ctggcatgcc gcatgacgct ctccgctgat ggcaccattg aagataatat
    58141 cgaattcttt gccgccacca tcgaatccaa agcgaagctg gtgtatgacc aggtttctga
    58201 ctggctggaa aataccggtg actggaagcc tgaaagtgaa gcaattgccg aacaagtccg
    58261 tttgctagcg caaatttgcc aacgccgcgg cgagtggcgt cataaccacg cactggtgtt
    58321 taaagatcgc ccggattacc gctttattct cggtgaaaaa ggtgaagtgc tggatatcgt
    58381 cgccgagcct cgtcgcattg ccaaccgtat cgtcgaagaa gcgatgattg ccgctaacat
    58441 ttgtgcggcc cgcgtactgc gcgataagct cggttttggc atctataacg tgcatatggg
    58501 ctttgatccg gcgaatgccg acgcgctggc agcgttgctg aaaacgcacg gtctgcatgt
    58561 cgatgccgaa gaagtgctca cgctggacgg tttctgcaaa ctgcgtcgtg aactggatgc
    58621 gcaaccaact ggtttcctcg acagccgcat tcgtcgcttc cagtcatttg ctgaaattag
    58681 cactgaaccc ggtcctcact ttggcctcgg tctggaagca tacgccacct ggacttcgcc
    58741 gatccgtaaa tatggcgaca tgatcaacca ccgtctgctg aaagcggtta tcaaaggcga
    58801 aactgcgacg cgcccacagg atgaaatcac tgtccaaatg gccgagcgtc gccgtctcaa
    58861 ccggatggca gaacgtgatg ttggcgactg gttatacgca cgcttcctga aagacaaagc
    58921 cgggaccgac acccgtttcg cggcggaaat tgtcgatatc agccgtggcg gcatgcgtgt
    58981 gcgtttggtt gataacggcg ctatcgcctt tattccggca cctttcttac acgctatgcg
    59041 cgatgaactg gtttgcagcc aggaaaacgg cactgtacaa attaaaggtg aaacggttta
    59101 caaagtaact gacgttattg acgtcaccat tgccgaagtc cgcatggaaa cccgcagcat
    59161 tattgcgcgc ccggtcgcgt aatctccttt cacggcccat tcctcatgga tgggccgttt
    59221 atttccccgc tctaccttcg ttatatccag gcagattttt taactgactt tcgtttgaaa
    59281 actggcgttt ttccaaaact ggattacaaa taaatatata aaaaaaacaa tgcgtttagc
    59341 cgccgttaat ttcatgtaag tcatgttgtt ccttttattt aaccgcggac tccgctgtta
    59401 accggaggat atgcatcatg aaaaccgtta gggagtccac aacgttgtac aactttctcg
    59461 gatcgcacaa tccatactgg cggttgacgg aaagcagcga tgttttgcgc ttttctacca
    59521 ccgaaaccac agaacctgat cgtacattgc agttatctgc cgaacaggct gctcgcatca
    59581 gggaaatgac ggtcatcacc tccagcctga tgatgagtct gaccgtcgat gaaagcgatc
    59641 tttctgtgca tctggtagga cgaaaaatca ataaacggga atgggctggc aacgcgtctg
    59701 catggcatga cacaccggcg gttgctcgtg atttatcaca cgggctttcc tttgctgagc
    59761 aggtagtttc tgaagcacat tccgcaatag tgattctcga cagccggggg aatatccaac
    59821 gcttcaatcg gttatgtgaa gattacacag ggttgaaaga acacgacgtc attgggcaaa
    59881 gcgtgtttaa actgtttatg agccgtcgtg aagctgcggc atccaggcgc aataaccgtg
    59941 tattttttcg aagcggcaat gcatatgaag tcgaactgtg gataccaaca tgtaaaggcc
    60001 agcggctgtt tctgtttcgc aataaatttg tccacagcgg cagtggcaaa aacgagattt
    60061 ttttaatctg ttccggcacc gacattaccg aagagcgccg cgctcaggag cgactgcgta
    60121 ttctggcaaa taccgacagt ttcaccggac tgccgaatcg taacgcaatg caggatttaa
    60181 tcgatcacgc tattaatcat gcagataaca ataaagttgg ggttgtgtat cttgatttgg
    60241 ataatttcaa aaaggtcaac gacgcctatg ggcatttgtt tggtgaccag ttattacgcg
    60301 acgtgtcatt ggctatttta agctgtctcg aacatgacca ggtgttggcg cgtccaggtg
    60361 gggatgagtt tctggtactg gcatccaaca cctcacaaag cgcgctggaa gcaatggcat
    60421 cacgaatttt gacccgctta cggctcccct ttcgcattgg tttaattgaa gtttatacca
    60481 gctgttcagt aggtattgca ctctctcccg aacatggttc agacagcacg gctattattc
    60541 gtcacgccga cacagcaatg tacacagcga aggaaggcgg acgaggacaa ttttgcgttt
    60601 ttaccccaga aatgaatcaa cgggtatttg aatatctctg gctggatacc aacttgcgta
    60661 aagcactgga aaacgatcag ttggttattc actatcaacc gaaaatcacc tggcgtggcg
    60721 aagtgcgcag tctggaagca ctagtacgtt ggcagtcacc tgaacgtggg ttgattccac
    60781 cgttggactt catttcctac gccgaagagt cagggctaat tgtgccttta ggccgttggg
    60841 tgattctcga tgtcgtacgc caggtggcaa agtggcggga taaaggcata aacctgcgag
    60901 tggcggtaaa tatttctgca cgtcagctcg ccgatcaaac cattttcacc gccctgaaac
    60961 aggttctcca ggaactcaat tttgaatact gccctataga tgttgaactg acagagagtt
    61021 gtctgattga gaatgatgaa ctggcactgt ctgttattca acaatttagc caactaggtg
    61081 cgcaagtgca tctggatgat tttggtaccg gctactcttc actttcgcaa ctggcgcgct
    61141 ttccgatcga tgccatcaaa cttgaccagg tttttgttcg agatattcac aaacaacctg
    61201 tctcgcagtc actggtccgg gcgatcgtcg ctgtggccca ggcattgaat cttcaggtga
    61261 tcgccgaagg tgtagagagt gcaaaggaag atgctttttt aaccaagaac gggatcaatg
    61321 agcggcaagg atttttgttt gccaaaccga tgcccgccgt cgccttcgaa cgctggtata
    61381 aacgctatct gaagcgcgca taagtagatg aatatatata cttagcgcta tatcccgtca
    61441 ttatatatcg tattcctgga tgataattag ccgttgctgt taaacacttc tcttttagcg
    61501 gcaatttttg cattattcat tttccacttt tgctataagg atgtccttga tgtctgaatt
    61561 tgatgcccag cgcgttgccg aacgtattga tattgtgtta gatatccttg tggcaggcga
    61621 ttaccactct gcgatccata atctcgagat acttaaagca gaactcttgc gccaggttgc
    61681 tgaatctacg ccagacatcc cgaaggcacc gtgggagatt taacaatcgt gccttcaggt
    61741 tttcctattt gaggatgccc ggcgcgtcag tattttttta tttagtatta taacgttata
    61801 agaattacag cgatgaattc ccgacaacaa actattctac agatggtcat tgaccagggt
    61861 caggttagcg taaccgatct ggcaaaagcc actggagttt ctgaagttac cattcgccag
    61921 gatctcaaca ccctcgaaaa actgagttac ctccgccgtg cacatggctt tgcagtttcg
    61981 cttgatagtg atgacgtcga aacccgtatg atgagcaact atacgctgaa gcgtgaactc
    62041 gccgagtttg ccgcgtctct ggttcaaccg ggcgaaacca tctttatcga aaatggtagc
    62101 agcaatgccc tgctcgcccg gactctgggc gaacagaaga aaaatgtcac tatcatcaca
    62161 gtcagcagct acattgcgca tttgctgaaa gacgcgcctt gtgaagtcat tttgctcggt
    62221 ggcgtgtacc agaaaaaaag cgaaagtatg gttggccctt tgacacgcca gtgcatccag
    62281 caggtacatt tcagcaaagc atttattggt attgatggct ggcaacctga aactggattc
    62341 accggtcgcg atatgatgcg taccgatgtg gtcaatgccg tgctggaaaa agagtgcgaa
    62401 gcgatagtcc tgactgacag ctcgaaattt ggtgctgtac attcatactc catcggtccc
    62461 gttgagcgat tcaatcgcgt gattaccgat tcgaaaatac gcgccagcga tctgatgcat
    62521 cttgagcaca gtaaactcac cgttcacgtc gttgacattt aaccatttcc cttctctacg
    62581 gatgatttgc agtttggcaa atcatccgct ctaagatgat tcctagttga taattaagac
    62641 tatttacctg ttattaacac gcttaagata taaaattatt atcagcgata taacaggaag
    62701 tcattatcac ctgcatgata taaccctgcg cgcgagcaga tttcacggaa taatttcacc
    62761 aggcttattc ttagctatta tagttataga gagcttactt ccgtgaatca taaattcagg
    62821 agagagtatt atgtttgtaa cgagcaaaaa aatgaccgcg gctgttctgg caattacttt
    62881 ggcaatgtct ctgagtgcct gttctaactg gtctaaacgg gaccgcaaca ccgcaatcgg
    62941 cgcgggtgca ggggcattag gcggtgcagt actgaccgat ggcagtacgt tgggtacatt
    63001 aggtggtgca gctgtcggtg gtgttattgg tcaccaggtc ggtaaataat cgtaaataaa
    63061 tgtcgattat cgactcgcaa ttataatacg cttgattctg ttcagtacga aataactgaa
    63121 taatacgcac agtaaaatat tcgaaagcca cggatatatc cgtggctttt tatgttaacc
    63181 gcctgcgagt tttaccttca tccctttcgc ttcaagtagt gattttaata aatcacgctt
    63241 atcgccctgg atttcaataa ctccatcttt aactgctccg ccgcagccgc attttttctt
    63301 cagttccgct gcaagttttg tcagttcggc atcatcgaga tcgacaccgg taatcaggca
    63361 aacgccctta cctttacgtc cactggtctg acgctgaata cgcaccacac cgtcgccttt
    63421 agggcgtaca ggggccgctt tgggctcatc aatacgtccg gtttccgttg agtagaccag
    63481 acggctgttg gaatcactca tcatgcactc cgctgtaaag aggcgttgat cgctttcagc
    63541 gtctgcgctg gatctaccga ttgcgttacc gggcgaccaa tcaccatata atcaacacca
    63601 gccgccaacg cctgttctgg cgtcataatg cggcgctggt caccagcatc actcccctgc
    63661 ggacgaatgc ccggtgtaac cagtttgaac tcctgaccga atacctgttt aaagcgcaca
    63721 gcttcctgag cagaacacac cacaccatca aggccacatt tttgcgtcag tgccgccaga
    63781 cgttctgcat aatctgcagg tgacagtgtc acgccaagat cggccaggtc gctagcttcc
    63841 atgctggtca acactgtcac agcaatcaaa agcggtgcat ctttgccaaa cggaaccagt
    63901 gcctcacgcg ctgcggtcat catacgcgcc ccaccagagg catgaacatt caccatccac
    63961 acgcctaagt cagctgcagc agcgacagcg tgcgctgcag tgttaggaat atcgtggaat
    64021 ttcaggtcaa gaaagatatc aaaaccacgc tgttgaagtt cgcgcacaaa ctgtggccca
    64081 aacaatgtaa acatctcttt gccgaccttc agacgacaat cgcgtgggtc gatcttgtcg
    64141 acaaaggaca gcgcgtcatc acgattatga taatcaaggg caacaaccac aggagaattc
    64201 gtaacagcgc gggaagaaga tgaagcagtt aacgtcatga ccagaccttc ttgatgatgg
    64261 gcaccggaag tggcgcgaaa caggtaaacg gcgagcattc tacccgtgga aataaaaaat
    64321 taacagttgc gatttttccc ctggcaggct ttttctgctc cgtcgttgcc ggagcacaat
    64381 ggaacatcaa taactagtat gttgtaacta aagtcggatt tttttaaaaa ttacaggcca
    64441 tcaagaccgc gaatcggttt aatggttgac caggcccgac aagatggaca atgccagtag
    64501 agggtgtatg cggtaaaacc acatttctgg cagcgataac gaggcttact acgtaccttt
    64561 tcgccaacca tgtcacgcag caccatcagg ctctctttgg cacgcccttc ttccgcttca
    64621 tttaagtggt aatccattaa cttatggaac acacgcatgg tcggatgacg ctgaagctgg
    64681 cgcgtaatat agacctgtgc ggcctcacta ccgtcgcgcg cttcgatgat atcagcaagc
    64741 atcaattcag catcggcacc ggtgttctct tccaccgcgc gttgcaggaa ttctgcccat
    64801 tcggcagttt tacccaactg ctgatagcag gtttgcaaca tttccagcgt ttcgctgacc
    64861 agttctctgt cctgggatat cacgcgttgc aaactttcga cggctttggc gtattctcct
    64921 ttcgccataa acacgcgtcc catcatgatg gatacgcggg cgctgttttt atctgccgcc
    64981 gccccttttt ttagcaaggt catggcacga tcgagatcgt cgctggccat atgctgcagg
    65041 gctaactcac agtagaaatg ggcaatttcg acgcgctgtt tatctttacc cagcttcacc
    65101 aggcgttcgg caacatcaat tgctttctgc cactcgctgg tagcctggta gatttgtagc
    65161 aactgttgca gcgcgccaat gcggaagtca gtttcatcgg tcagctgatt gaacatgtct
    65221 tccgcgcggt cgtataaccc ggcggccatg taatcacgcc ccagttgttg aatcgccaac
    65281 agacgctgtt cataggtcag tgaggcgctt tccattaggg tctgatggat gcgaatagcg
    65341 cgatcaactt cgccacgcga acggaacagg tttccgagcg taaggtgggc ttcaacggtg
    65401 cctgtatcct ctttaagcat atcgagaaac aggtctaccg ctttatcctg ttgattacta
    65461 agcaggaagt taacccccgc tacgtaatca cgcgacaagc ggttggcttc atcttgcttg
    65521 ttttgttgcg cacttctgcg gcccatatac cagccatagg cagcggctac aggcaaaagc
    65581 agaaacaaca actccagcat agaaagttat tccttcgccg ctgacgagtg cggcactaca
    65641 gccacgtcag tcgcgggtga aagctggttt tccagtcgct ttattttacg ttcagcgcgc
    65701 gccagggaaa cacgaactcg cagccagaac aggccacaaa tcaaccaacc gatagcaaac
    65761 cccgcagcaa acaataccgc caacaatgtg gaaatacggt actccccttg cgctaacaga
    65821 taattaaacg tcacctgttg atcgttctgc gcacccaacg tgaccgaaat cacgaagatc
    65881 gctaacacca gtaagaaaat gagtaaatat ttcacattac ttcccgttat gtggttagag
    65941 cgaataaatt atggtcaact taccgcaata gccctttaaa gtaccatttt cgcgccgtct
    66001 gcgaaatggc atccacgcgc attgttgatg atttaagggc taaattcgga aaatcaacca
    66061 gcgttaactt tcttgttctc gttgcgctat ttcgcgattt tcttccgcag gtggtgttaa
    66121 tggcccacaa attcgttgcg caagccacgt tgccaccgcc accagcgccc acgaaatcaa
    66181 cgtagctact accagatcgc gtggccaatg catcccgagc agcaggcggc ttcccatgac
    66241 tcccgttgcc cagaccagca agatagcaat ggttaacgtt cgccgacgcg gccacagcaa
    66301 accaacggcc agcagtgccc aactggcagc aaacatcgtg tgaccggaag gaaaggcaaa
    66361 ccccgtctct ttctgccagt gtgaacgcaa atattgtggg atatttttct cttcagccaa
    66421 ctgttctttc actaaatttc cgcgttctgc tcgctttaaa gtgtagaact catcaaccgg
    66481 aatatgatgt gttttttcca gccagataac aaaaggtcgt ggttcctgga ctttgtcttt
    66541 gatccaggat ttaacgcctt gtcccacaag gattgcggcc gccagaatgg caaataatac
    66601 aaaggcagcc ttaatgcgaa aacgcagaca ccagagaaac cagccgaata aaatcaaatg
    66661 tgtaatgacg ccccagggct gggtgacagt ttcagtaacc caaaaagccg cttttagtag
    66721 ccaactttgt tctccaggtt gccaacgcca gccagaaatc catacggcta ctggcatgac
    66781 aagcaatagt gcagctccca ctgcggtacg tctggcaatc gaacgcatgg cctctccttt
    66841 ttgataagtc ccacaatcat aactgaaaac gccagttcca ggaaaaattg acagatttgt
    66901 gccattccgt gaacgatcga cgcgtcgtga ttaggtgaac cccttctcgt tatggcaaaa
    66961 taagccaata cagaaccagc attatctgga gaatttcatg cagcttaaac gtgtggcaga
    67021 agccaaactg ccaaccccat ggggcgattt cctgatggtg ggatttgaag aactggcaac
    67081 cggacacgat catgtcgcgc tagtctatgg cgatatttcc gggcataccc cggtacttgc
    67141 gcgcgtccat tccgaatgtc tgaccggtga cgccctgttc agcttgcgct gcgattgtgg
    67201 cttccagctc gaagcggcat tgacgcaaat tgccgaggaa ggccgtggta ttttgctgta
    67261 tcaccgtcag gaaggtcgta acattggtct gctgaataaa atccgcgctt acgcactgca
    67321 ggatcaaggt tacgataccg tagaggctaa ccaccagtta ggcttcgccg ctgatgagcg
    67381 cgacttcact ctttgcgctg atatgttcaa actccttggc gtcaatgaag tccgcttgtt
    67441 aaccaataac ccgaaaaaag tcgaaattct gaccgaagca gggattaata ttgttgaacg
    67501 cgtaccattg attgtaggtc gtaaccccaa taacgaacat tatctcgata ccaaagccga
    67561 gaaaatgggc catttgctga acaaataacc ctcttgcatt gtgtaattag tttgcttgcc
    67621 ggaagcaaaa taaccggcaa gcaaatactt gttacttcaa catattacga atgacataat
    67681 gcaaaatgcc gtcgttctgg tagtaggtca actccgtcgc ggtgtcgata cgacaacggc
    67741 agggtacgac ctcctggcta ccatccgcgc gcgtaagcgt caccggaacc gtcgcaccgg
    67801 gttgtagatt ttgcagatcg ccaatatcaa tcttctcttc cccggttagt cctaacgttt
    67861 tacgcgttac gccttgcgga aattccagcg gcaggatgcc catgccaatt aaattcgaac
    67921 ggtgaattcg ttcaaacgat tcggcaatca ccacacgaat tccaagcaga cgaggacctt
    67981 ttgccgccca gtcacggctt gagcctgatc catactcttt cccggcaatc accgccagcg
    68041 gcgtttgttc ctgcttatag cgcatcgcag catcataaat agagactacg tcgctgtcag
    68101 gtaaatgccg cgtcatcccc ccttcaacgc caggcaccat ttcattacgg atgcgaatat
    68161 tggcgaaggt gccgcgcatc atcacttcat ggttaccacg ccgcgaaccg taggagttaa
    68221 agtcttttcg ctcaacaccc cgaccttgta gatatcgacc cgctgggctg tcgggcttaa
    68281 tactgcccgc cggagagata tggtcagtgg tgactgaatc ccccagcatt gcgaggatcc
    68341 gcgcaccgtg aatatcttcc actggtgctg gtgttgcctg catttcatca aagaaaggcg
    68401 ataagcgaat ataggttgag tcctcctgcc aaccgtaggt atcggatcgt gtgacgttaa
    68461 tttccttcca ctctgctgtg ccttcaaaaa cttctgcgta ctctttgcgg aacatttctg
    68521 tggagacttg ttctaccgca cgggcaattt cttgtgccga tggccagata tctttcagat
    68581 ataccggatc gcctttacga tcatggccga taggctcaga agccaggttg atattcatat
    68641 ttcccgccag cgcataggca accaccagcg gcggcgaggc cagccagttg gttttaacca
    68701 gcggatggat acggccttca aagttacggt tgccggacag cactgcaccg acggttaaat
    68761 cgcctttttt gattgccgtt tcgataggat cgggcagcgg cccagagtta ccaatacagg
    68821 tggtacaacc gtatcccaca aggttaaacc ccagttcgtc gagatacggt gtcaatttcg
    68881 cttttgccag ataatcagaa acaactttcg agcctggtgc cagcgacgct ttgacccacg
    68941 gttgccgctt gaggccaaga gttacggctt ttttcgccag caagcctgca gccatcagca
    69001 cacttgggtt agaggtgttg gtgcacgagg ttatcgcagc aatgaccaca gcgccatcag
    69061 gtaactgata ctgatgtccg ttcataacat aatcgaccgg ctggcgatct ttatgcgtgg
    69121 cattcacttc cagttcgtta ctggcggcaa atgcttttgg tacatcgggc agtgcaacgc
    69181 gatcctgtgg gcgttttggc cctgccaggc tcgcttcaac gtcattcata tccagttcta
    69241 acgtactggt aaaaatcggt tcatcgcccg ggttacgcca catgccctgc gctttggcat
    69301 atttttcgac caactcaacc tgatcttcgc tgcgcccgct taaacgcatg taatcgaggg
    69361 ttacagcatc gattgggaag aagccacagg tggcaccata ttctggcgac atattggcaa
    69421 tggtggcgcg atccgccagc ggtagtgaat ccagaccatc accataaaat tcgacgaatt
    69481 tccccaccac gccatgtttg cgcagcattt gggtaacagt gagaaccagg tctgtggcgg
    69541 taataccttc acgtaattta ccggtaagtt taaagcccac tacgtccggg ataagcatgg
    69601 aaaccggctg gcctaacatt gcggcttctg cttcgatccc acccacgccc caccccagca
    69661 cgccaaggcc gttgatcata gtggtgtgcg agtcagtacc aacgagtgta tccggataag
    69721 caatccattc accgtcctgc aattcactcc acactgcttt gccgagatat tcgaggttaa
    69781 cctgatggca aatgcctgtg cctggcggca cgacgctaaa ccgactgaac gcttgctttc
    69841 cccatttcag gaacacataa cgttcgtggt tgcgctccat ttccaggcgt acgttttctt
    69901 caaatgcctc atcatcacca aaacgatcga cggtcaccga gtggtcaatg accaggtcga
    69961 ccggtgagag cgggttaacc tttgcagtat cgccgccgag gcgtttaacc gcttcgcgca
    70021 ttgccgccag atcaacaacg gcaggtacgc cggtaaagtc ctgcatcagc acccttgccg
    70081 ggcggtaggc aatttcacgg tcagcatggg catttttcag ccatcctgcc agcgcgtgga
    70141 tatcctcttc ggtaaccgag ttaccatcct gccagcgcag caggttttcg agcaaaactt
    70201 tgagtgactt gggtagacgg gtgatatcgc ccagtgattt agcagcaagc ggcaggctgt
    70261 agtagtggta agttttatct ttggcctgca acgtgtcctt actggcttct cgtagggttg
    70321 acgacatagc tcctccttaa tgacagggtt gcgaagccct gattctcttc agggtgaata
    70381 ttaaagataa cacaaacatc gcgtaacgat ttgataacaa cccaaattga taaaagagga
    70441 gaaaccttgt ggcaaagcaa atgacaaccc cgccgcagcg gggtcaggga tcagtgagta
    70501 atgatccaga ggagttcggc ccagaagagc atcgacagag aaaatgcgcc catccaggac
    70561 caatatttca tatttgtgtt gatcataggc tacaccctta acggttatta ctttgtgttg
    70621 agataactca acctgttatt gctgcggtgt attaaaagcc ctgtaattca ttaaaaatgg
    70681 gtaataagaa tgatgataat tattgtttcg gctgatcatc ttcagcaaac agttctataa
    70741 aggcttgctg ctgcgatgtt agttgccagt cgtctggaat aacgacggaa cattcccgtt
    70801 tcgtttctgt ttcattgtca ctatgctggc ataatggtga cgactccagt tgctcctgac
    70861 cgaccatagt tatccccaca ctttccaaat cagcagtgcg acaaccaccc agaaaagtgc
    70921 agaaccaaga aaaaccgcca gccaggcttt acgttttaat tcaggatcgc ggcggggttc
    70981 ctgatttcca gaaggcattg ctaacctcat ataatcgcca tcacttatca gcaagacgtt
    71041 gaacgattgg tgtaattaat tatacgatga gacaaatcct aaagaaactt tagtcaaaaa
    71101 tccagcgaat ttacgaatcg tctccagtga ataattcaat cttttgacct gaaatagata
    71161 tttgaaaact ggaatttgcg aggcgggtaa ttagacacta ctttacaggt aatttaccct
    71221 ataaatatga gggataaaaa tattgtgcac ggtatacaaa taagtaacaa aagagaatat
    71281 attccggcac cttcgctaca taaaaggtgc cgaaaataac gcaagaaatt atttttccgg
    71341 cagttttata tctttaaaca tgacctcaat ttcttcattg gagcgcaatg cgacagccgc
    71401 atcaacgaca tcacgcgtta aatgcggtgc aaaacgctga atgaaatcat acatataact
    71461 gcgcaaaaaa gtactacggc gaaaaccaat tttggttgta ctgtggctga agatatcgtg
    71521 agcatcaacg cgcacaaggt cgggatcggc gaccggatcc accgccatgc tggcaatgac
    71581 ccctaccccc agccctaacc ggacgtaagt tttaatgacg tcagcatccg ttgccgtgaa
    71641 aacgatacgc ggcgttaacc ctgcgcggtt aaaggcagta tccagttctg aacgtccggt
    71701 aaagccgaag gtatatgtca ccaacggata ttgcgccagt tcttcaatgg taatggcttt
    71761 tttgcctgcc agcgggtgat ccggagtgac tacaatagcc cgattccagt ggtagcacgg
    71821 taacatcact aaatcttcat acagatgcag cgcttctgtg gcgatagcga aatcggcatt
    71881 gcctttagag acggcatcag caatttgtgt cggcgagccc tggtgcatat gcaaagaaac
    71941 gcgaggataa cgctcaataa agcctttgat gacgtttggt aatgcgtagc gtgcctgggt
    72001 atgcgtggtg gcgatataca gcgagccttt atccggccag gtgtgctctc cggcaaccga
    72061 ttttatggca tcgactttcg acaggacttc gcgagcgata cgaattattt cttgtcctgc
    72121 tggcgttacc tgcgtcaggt gcttgccgct tcgggaaaaa atttgaatgc ctagctcgtc
    72181 ttccagcatt ctgacttgtt tactgatccc gggttgtgat gtgtaaagtc cttccgctgt
    72241 tgatgagaca ttcaggttat gattgaccac ctcaacaata tagcgaagtt gttgtaattt
    72301 catgttaaac catccactta gacgccatca gaacatcgtt ttttcgtcac acctggcggg
    72361 tgactaatac gatttaataa ta
//
LOCUS       DAZFQD010000008        67136 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.008, whole genome shotgun sequence.
ACCESSION   DAZFQD010000008 DAZFQD010000000
VERSION     DAZFQD010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 67136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 67136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..67136
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.008"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(22..2211)
                     /gene="pdeA"
                     /locus_tag="ACHKTW_000566"
     CDS             complement(22..2211)
                     /gene="pdeA"
                     /locus_tag="ACHKTW_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HFO6461648.1"
                     /translation="MFVEHNLIKNIKIFTLAFTLTVVLIQLSRFISPLAIIHSSYIFL
                     AWMPLCVMLSILFIFGWRGVVPVLCGMFCTNLWNFHLSFLQTAVMLGSQTFVVLCACA
                     ILRWQLGTRWRYGLTSRYVWQRLFWLGLVTPIGIKCSMYLVGSFFDFPLKISTFFGDA
                     DAIFTVVDLLSLFTAVLIYNMLFYYLTRMIVSPHFAQILWRRDIAPSLGKEKRAFTLS
                     WLAALSVLLLLLCTPYENDFIAGYLVPVFFIIFTLGVGKLRYPFLNLTWAVSTLCLLN
                     YNQNFLQGVETEYSLAFILAVLISFSVCLLYMVRIYHRSEWLNRRWHLQALTDPLTLL
                     PNFRALEQAPEQEAGKSFCCLRIDNLEFMSRHYGLMMRVHCIRSICRTLLPLMQENEK
                     LYQLPGSELLLVLSGPETEGRLQHMVNILNSRQIHWNNTGLDMGYGAAWGRFDGNQET
                     LQPLLGQLSWLAEQSCAHHHVLALDSREEMVSGQTTKQVLLLNTIRTALDQGDLLLYA
                     QPIRNKEGEGYDEILARLKYDGGIMTPDKFLPLIAQFNLSARFDLQVLESLLKWLATH
                     PCDKKGPRFSVNLMPLTLLQKNIAGRIIRLFKRYHISPQSVILEITEEQAFSNAESSM
                     YNIEQLHKFGFRIAIDDFGTGYANYERLKRLQADIIKIDGVFVKDIVTNTLDAMIVRS
                     ITDLAKAKSLSVVAEFVETQQQQALLHKLGVQYLQGYLIGRPQPLAD"
     gene            complement(2419..2494)
                     /locus_tag="ACHKTW_000567"
     tRNA            complement(2419..2494)
                     /locus_tag="ACHKTW_000567"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2459..2461),aa:Ala,seq:ggc)
     gene            complement(2534..2609)
                     /locus_tag="ACHKTW_000568"
     tRNA            complement(2534..2609)
                     /locus_tag="ACHKTW_000568"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2574..2576),aa:Ala,seq:ggc)
     gene            2830..3189
                     /gene="yfeC"
                     /locus_tag="ACHKTW_000569"
     CDS             2830..3189
                     /gene="yfeC"
                     /locus_tag="ACHKTW_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HFO6461649.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            3191..3583
                     /gene="yfeD"
                     /locus_tag="ACHKTW_000570"
     CDS             3191..3583
                     /gene="yfeD"
                     /locus_tag="ACHKTW_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HFO6461650.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWIREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLMEAEERIAEYAPGARAPAYRQIINAIDNMTD
                     IEQEKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(3635..5050)
                     /gene="gltX"
                     /locus_tag="ACHKTW_000571"
     CDS             complement(3635..5050)
                     /gene="gltX"
                     /locus_tag="ACHKTW_000571"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFO6461651.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALDFIAERENQQ"
     gene            5309..5384
                     /locus_tag="ACHKTW_000572"
     tRNA            5309..5384
                     /locus_tag="ACHKTW_000572"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5342..5344,aa:Val,seq:tac)
     gene            5429..5504
                     /locus_tag="ACHKTW_000573"
     tRNA            5429..5504
                     /locus_tag="ACHKTW_000573"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5462..5464,aa:Val,seq:tac)
     gene            5551..5626
                     /locus_tag="ACHKTW_000574"
     tRNA            5551..5626
                     /locus_tag="ACHKTW_000574"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5584..5586,aa:Val,seq:tac)
     gene            5631..5706
                     /locus_tag="ACHKTW_000575"
     tRNA            5631..5706
                     /locus_tag="ACHKTW_000575"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5664..5666,aa:Lys,seq:ttt)
     gene            complement(5815..6699)
                     /gene="xapR"
                     /locus_tag="ACHKTW_000576"
     CDS             complement(5815..6699)
                     /gene="xapR"
                     /locus_tag="ACHKTW_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator XapR"
                     /protein_id="HFO6461652.1"
                     /translation="MERVYRTDLKLLRYFLAVAEELHFGRAAARLNMSQPPLSIHIKE
                     LENQLGTQLFIRHSRSVVLTHAGKILMEESRRLLVNANNVLARVEQIGRGEAGRIELG
                     VVGTAMWGRMRPVMRRFLRENPNVEVLFREKMPAMQMALLERRELDAGIWRMATEPPT
                     GFTSLRLHESAFLVAMPEEHHLSSFSTVPLEALRDEYFVTMPPVYTDWDFLQRVCQQV
                     GFSPVVIREVNEPQTVLAMVSMGIGITLIADSYAQMNWPGVIFRPLKQRIPADLYIVY
                     ETQQVTPAMVKLLAALTQ"
     gene            complement(6740..6898)
                     /locus_tag="ACHKTW_000577"
     CDS             complement(6740..6898)
                     /locus_tag="ACHKTW_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1427 family protein"
                     /protein_id="HFO6461653.1"
                     /translation="MKDISFSSGVGFGIGALFTIVRLPIPVPNVLPGILSIVFMYVGY
                     LVVKYFMP"
     gene            complement(6951..8207)
                     /gene="xapB"
                     /locus_tag="ACHKTW_000578"
     CDS             complement(6951..8207)
                     /gene="xapB"
                     /locus_tag="ACHKTW_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthosine/proton symporter XapB"
                     /protein_id="HFO6461654.1"
                     /translation="MSIAMRLKVMSFLQYFIWGSWLVTLGSYMINTLHFTGANVGMVY
                     SSKGIAAIIMPGIMGIIADKWLRAERAYMLCHLVCAGVLFYAASVTDPDMMFWVMLVN
                     AMAFMPTIALSNSVSYSCLAQAGLDPVTAFPPIRVFGTVGFIVAMWAVSLLHLELSSL
                     QLYIASGASLLLSAYALTLPKIPVAEKKATTSLASKLGLDAFVLFKNPRMAIFFLFAM
                     MLGAVLQITNVFGNPFLHDFARNPEFADSFVVKYPSILLSVSQMAEVGFILTIPFFLK
                     RFGIKAVMLMSMVAWTLRFGFFAYGDPSTTGFILLLLSMIVYGCAFDFFNISGSVFVE
                     QEVDSSIRASAQGLFMTMVNGVGAWVGSILSGMAVDYFSVDGVKDWQTIWLVFAGYAL
                     FLAVIFFFGFKYNHDPEKIKHRAVAH"
     gene            complement(8267..9100)
                     /gene="xapA"
                     /locus_tag="ACHKTW_000579"
     CDS             complement(8267..9100)
                     /gene="xapA"
                     /locus_tag="ACHKTW_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0055086 - nucleobase-containing small
                     molecule metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthosine phosphorylase"
                     /protein_id="HFO6461655.1"
                     /translation="MSQVQFSHNPLFCIDIIKTYKPDFTPRVAFILGSGLGALADQIE
                     NAVAISYEKLPGFPVSTVHGHAGELVLGHLQGVPVVCMKGRGHFYEGRGMTIMTDAIR
                     TFKLLGCELLFCTNAAGSLRPEVGAGSLVALKDHINTMPGTPMVGLNDDRFGERFFSL
                     ANAYDAEYRALLQKVAKEEGFPLTEGVFVSYPGPNFETAAEIRMMQIIGGDVVGMSVV
                     PEVISARHCDLKVVAVSAITNMAEGLSDVKLSHAQTLAAAELSKQNFINLICGFLRKI
                     A"
     gene            9349..10113
                     /gene="yfeN"
                     /locus_tag="ACHKTW_000580"
     CDS             9349..10113
                     /gene="yfeN"
                     /locus_tag="ACHKTW_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpK"
                     /protein_id="HFO6461656.1"
                     /translation="MKKHLLTLTLSSILAIPVVSHAQFKGGFADIGVHYLDWTSRTTE
                     KSSTKSHKDDFGYLEFEGGANFSWGEMYGFFDWENFYNGRHNKPGSEQRYTFKNTNRI
                     YLGDTGFNLYLHAYGTYGSANRVNFHDDMFLYGIGYNFTGSGWWFKPFFAKRYTDQTY
                     YTGDNGYVAGWVAGYNFMLGSEKFTLTNWNEYEFDRDATYAAGNGGKEGLNGAVALWW
                     NATSHITTGIQYRYADDKLGEDFYQDAIIYSIKFNF"
     gene            complement(10152..11078)
                     /gene="yfeR"
                     /locus_tag="ACHKTW_000581"
     CDS             complement(10152..11078)
                     /gene="yfeR"
                     /locus_tag="ACHKTW_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO6461657.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGREGDPLYQI"
     gene            11168..12166
                     /gene="yfeH"
                     /locus_tag="ACHKTW_000582"
     CDS             11168..12166
                     /gene="yfeH"
                     /locus_tag="ACHKTW_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HFO6461658.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGAGGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLGFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSTDKA"
     gene            complement(12163..12381)
                     /gene="ypeB"
                     /locus_tag="ACHKTW_000583"
     CDS             complement(12163..12381)
                     /gene="ypeB"
                     /locus_tag="ACHKTW_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HFO6461659.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(12383..14398)
                     /gene="ligA"
                     /locus_tag="ACHKTW_000584"
     CDS             complement(12383..14398)
                     /gene="ligA"
                     /locus_tag="ACHKTW_000584"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFO6461660.1"
                     /translation="MESIEQQLTELRTTLRHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KNNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGLPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKSAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            complement(14469..15455)
                     /gene="zipA"
                     /locus_tag="ACHKTW_000585"
     CDS             complement(14469..15455)
                     /gene="zipA"
                     /locus_tag="ACHKTW_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HFO6461661.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYQPPYASAQPR
                     QPVQQPPEAQVPPQHAPRPAQPVQQPAYQPQPEQPLQQPVSPQVAPAPQPVHSAPQPA
                     QQAFQPAEPVAAPQPEPVAEPAPVTDKPKRKEAVIIMNVAAHHGSELNGELLLNSIQQ
                     AGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQVPSYG
                     DELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            15685..16446
                     /gene="cysZ"
                     /locus_tag="ACHKTW_000586"
     CDS             15685..16446
                     /gene="cysZ"
                     /locus_tag="ACHKTW_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HFO6461662.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTLMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFAWYLPRAIVLLILYLIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            16631..17602
                     /gene="cysK"
                     /locus_tag="ACHKTW_000587"
     CDS             16631..17602
                     /gene="cysK"
                     /locus_tag="ACHKTW_000587"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFO6461663.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQALAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            17986..18243
                     /gene="ptsH"
                     /locus_tag="ACHKTW_000588"
     CDS             17986..18243
                     /gene="ptsH"
                     /locus_tag="ACHKTW_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HFO6461664.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            18288..20015
                     /gene="ptsI"
                     /locus_tag="ACHKTW_000589"
     CDS             18288..20015
                     /gene="ptsI"
                     /locus_tag="ACHKTW_000589"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HFO6461665.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            20056..20565
                     /gene="crr"
                     /locus_tag="ACHKTW_000590"
     CDS             20056..20565
                     /gene="crr"
                     /locus_tag="ACHKTW_000590"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFO6461666.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
     gene            complement(20607..21458)
                     /gene="pdxK"
                     /locus_tag="ACHKTW_000591"
                     /gene_synonym="thiJ"
     CDS             complement(20607..21458)
                     /gene="pdxK"
                     /locus_tag="ACHKTW_000591"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HFO6461667.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
     gene            21563..21931
                     /gene="yfeK"
                     /locus_tag="ACHKTW_000592"
     CDS             21563..21931
                     /gene="yfeK"
                     /locus_tag="ACHKTW_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001592994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HFO6461668.1"
                     /translation="MKKIICLVITLLMTLPAYAKLTAHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQTDKTVPAQ"
     gene            complement(21934..22845)
                     /gene="cysM"
                     /locus_tag="ACHKTW_000593"
     CDS             complement(21934..22845)
                     /gene="cysM"
                     /locus_tag="ACHKTW_000593"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HFO6461669.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSRFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNASLVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(22979..24076)
                     /gene="cysA"
                     /locus_tag="ACHKTW_000594"
     CDS             complement(22979..24076)
                     /gene="cysA"
                     /locus_tag="ACHKTW_000594"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HFO6461670.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNIAFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPNQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQRGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            complement(24066..24941)
                     /gene="cysW"
                     /locus_tag="ACHKTW_000595"
     CDS             complement(24066..24941)
                     /gene="cysW"
                     /locus_tag="ACHKTW_000595"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HFO6461671.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            complement(24941..25774)
                     /gene="cysT"
                     /locus_tag="ACHKTW_000596"
     CDS             complement(24941..25774)
                     /gene="cysT"
                     /locus_tag="ACHKTW_000596"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HFO6461672.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLAQMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            complement(25774..26790)
                     /gene="cysP"
                     /locus_tag="ACHKTW_000597"
     CDS             complement(25774..26790)
                     /gene="cysP"
                     /locus_tag="ACHKTW_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HFO6461673.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKNKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRN"
     gene            complement(26961..27752)
                     /gene="ucpA"
                     /locus_tag="ACHKTW_000598"
     CDS             complement(26961..27752)
                     /gene="ucpA"
                     /locus_tag="ACHKTW_000598"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HFO6461674.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DDDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVCTPMAESIARQSNPEDPESVLTEMAKAIPM
                     RRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            complement(27881..28738)
                     /gene="murR"
                     /locus_tag="ACHKTW_000599"
     CDS             complement(27881..28738)
                     /gene="murR"
                     /locus_tag="ACHKTW_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HFO6461675.1"
                     /translation="MLYLTKISNAGSEFTENEQKIADFLQANVSELQSVSSRKMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATALHLHSSITSDDSLEVIA
                     RKLNREKELALEQTCALFDYARLQKIIDVISKAQFIQITGLGGSALVGRDLSFKLMKI
                     GYRVACEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQGATVIAITSL
                     ADSPLRRLAHFTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQLNDVESLKMIQR
                     SSELTQRLK"
     gene            28902..29798
                     /gene="murQ"
                     /locus_tag="ACHKTW_000600"
     CDS             28902..29798
                     /gene="murQ"
                     /locus_tag="ACHKTW_000600"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFO6461676.1"
                     /translation="MQLEKMITEGSNTASAEIDRVSTLEMCRIINDEDKTVPLAVERV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGVNDLKNINLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            29802..31226
                     /gene="murP"
                     /locus_tag="ACHKTW_000601"
     CDS             29802..31226
                     /gene="murP"
                     /locus_tag="ACHKTW_000601"
                     /EC_number="2.7.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HFO6461677.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PNIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSELLGEAPVQDAAEIAAQNKRQL
                     KAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPAATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAAWACARIEGMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPFGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNN
                     LFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFITACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVCGFIFTTLFGCRNVNLD"
     gene            31231..32535
                     /gene="pbp4b"
                     /locus_tag="ACHKTW_000602"
     CDS             31231..32535
                     /gene="pbp4b"
                     /locus_tag="ACHKTW_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin binding protein PBP4B"
                     /protein_id="HFO6461678.1"
                     /translation="MKRTMLYLSLLAVSCSVSAAKYPVLTESSPEKAGFNVERLNQMD
                     RWISQQVDAGYPGVNLLIIKDNQIVYRKAWGAAKKYDGSVLMEQPVKATTGTLYDLAS
                     NTKMYATNFALQKLMSEGKLHPDDRIAKYIPGFADSPNDTIKGKNTLRISDLLHHSGG
                     FPADPQYPNKAVAGALYSQDKGQTLEMIKRTPLEYQPGSKHIYSDVDYMLLGFIVESV
                     TGQPLDRYVEESIYRPLGLTHTVFNPLLKGFKPQQIAATELNGNTRDGVIHFPNIRTS
                     TLWGQVHDEKAFYSMGGVSGHAGLFSNTGDIAVLMQTMLNGGGYGDVQLFSAETVKMF
                     TTSSKEDATFGLGWRVNGNATMTPTFGTLASPQTYGHTGWTGTVTVIDPVNHMAIVML
                     SNKPHSPVADPQKNPNMFESGQLPIATYGWVVDQVYAALKQK"
     gene            complement(32593..33492)
                     /gene="yfeX"
                     /locus_tag="ACHKTW_000603"
     CDS             complement(32593..33492)
                     /gene="yfeX"
                     /locus_tag="ACHKTW_000603"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HFO6461679.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDAGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(33588..34163)
                     /gene="yfeY"
                     /locus_tag="ACHKTW_000604"
     CDS             complement(33588..34163)
                     /gene="yfeY"
                     /locus_tag="ACHKTW_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HFO6461680.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTANGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWSGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            complement(34224..34673)
                     /gene="yfeZ"
                     /locus_tag="ACHKTW_000605"
     CDS             complement(34224..34673)
                     /gene="yfeZ"
                     /locus_tag="ACHKTW_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HFO6461681.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRATFPRTWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            complement(34660..35085)
                     /gene="ypeA"
                     /locus_tag="ACHKTW_000606"
     CDS             complement(34660..35085)
                     /gene="ypeA"
                     /locus_tag="ACHKTW_000606"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HFO6461682.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            35299..36168
                     /gene="amiA"
                     /locus_tag="ACHKTW_000607"
     CDS             35299..36168
                     /gene="amiA"
                     /locus_tag="ACHKTW_000607"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HFO6461683.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            36172..37071
                     /gene="hemF"
                     /locus_tag="ACHKTW_000608"
     CDS             36172..37071
                     /gene="hemF"
                     /locus_tag="ACHKTW_000608"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HFO6461684.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCLPFGEDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(37077..38129)
                     /gene="eutR"
                     /locus_tag="ACHKTW_000609"
     CDS             complement(37077..38129)
                     /gene="eutR"
                     /locus_tag="ACHKTW_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
                     /protein_id="HFO6461685.1"
                     /translation="MKKTRTANLHHLYHEPLPENLKLTPKVEVDNVHQRQTTDVYEHA
                     LTITAWQQIYDQLHPGKFHGEFTEILLDDIQVFREYTGLALRQSCLVWPNSFWFGIPA
                     TRGEQGFIGSQCLGSAEIATRPGGTEFELSTPDDYTILGVVLSEDVITRQANFLHNPD
                     RVLHMLRSQSALEVKEQHKAALWGFVQQALATFCENPENLHQPAVRKVLGDNLLMAMG
                     AMLEDAQPMVTAESISHQSYRRLLSRAREYVLENMSEPVTVLDLCNQLHVSRRTLQNA
                     FHAILGIGPNAWLKRIRLNAVRRELISPWSQSTTVKDAAMQWGFWHLGQFATDYQQLF
                     AEKPSLTLHQRMREWG"
     gene            complement(38175..38675)
                     /gene="eutK"
                     /locus_tag="ACHKTW_000610"
     CDS             complement(38175..38675)
                     /gene="eutK"
                     /locus_tag="ACHKTW_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HFO6461686.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVREPD
                     APVIVAESADELLALLTSVRQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLCKRSSR
                     YRLKPH"
     gene            complement(38688..39347)
                     /gene="eutL"
                     /locus_tag="ACHKTW_000611"
     CDS             complement(38688..39347)
                     /gene="eutL"
                     /locus_tag="ACHKTW_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HFO6461687.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            complement(39357..40244)
                     /gene="eutC"
                     /locus_tag="ACHKTW_000612"
     CDS             complement(39357..40244)
                     /gene="eutC"
                     /locus_tag="ACHKTW_000612"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit beta"
                     /protein_id="HFO6461688.1"
                     /translation="MDQKQIEEIVRSVMASMGQTAPAPSEAKCATTNCAAPVTSESCA
                     LDLGSAEAKAWIGVENPHRADVLTELRRSTVARVCTGRAGPRPRTQALLRFLADHSRS
                     KDTVLKEVPEEWVKAQGLLEVRSEISDKNLYLTRPDMGRRLCAEAVEALKAQCVANPD
                     VQVVISDGLSTDAITVNYEEILPPLMAGLKQAGLKVGTPFFVRYGRVKIEDQIGEILG
                     AKVVILLVGERPGLGQSESLSCYAVYSPRMATTVEADRTCISNIHQGGTPPVEAAAVI
                     VDLAKRMLEQKASGINMTR"
     gene            complement(40265..41626)
                     /gene="eutB"
                     /locus_tag="ACHKTW_000613"
     CDS             complement(40265..41626)
                     /gene="eutB"
                     /locus_tag="ACHKTW_000613"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit alpha"
                     /protein_id="HFO6461689.1"
                     /translation="MKLKTTLFGNVYQFKDVKEVLAKANELRSGDVLAGVAAASSQER
                     VAAKQVLSEMTVADIRNNPVIAYEDDCVTRLIQDDVNETAYNQIKNWSISELREYVLS
                     DETSVDDIAFTRKGLTSEVVAAVAKICSNADLIYGAKKMPVIKKANTTIGIPGTFSAR
                     LQPNDTRDDVQSIAAQIYEGLSFGVGDAVIGVNPVTDDVENLSRVLDTIYGVIDKFNI
                     PTQGCVLAHVTTQIEAIRRGAPGGLIFQSICGSEKGLKEFGVELAMLDEARAVGAEFN
                     RIAGENCLYFETGQGSALSAGANFGADQVTMEARNYGLARHYDPFIVNTVVGFIGPEY
                     LYNDRQIIRAGLEDHFMGKLSGISMGCDCCYTNHADADQNLNENLMILLATAGCNYIM
                     GMPLGDDIMLNYQTTAFHDTATVRQLLNLRPSPEFERWLESMGIMANGRLTKRAGDPS
                     LFF"
     gene            complement(41638..43041)
                     /gene="eutA"
                     /locus_tag="ACHKTW_000614"
     CDS             complement(41638..43041)
                     /gene="eutA"
                     /locus_tag="ACHKTW_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HFO6461690.1"
                     /translation="MNTRQLLSVGIDIGTTTTQVIFSHLELVNRAAVSQVPRYEFIKR
                     EISWQSPVFFTPVDKQGGLKEAELKTLILEQYQAAGIAPESVDSGAIIITGESAKTRN
                     ARPAVMALSQSLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANY
                     ALFDAGKISGTACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTDARSLTGAQL
                     VQVTRRMAALIVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPADP
                     FCFADIGPLLATALHDHPRLREMNVQFPAQTVRATVIGAGAHTLSLSGSTIWLEGVQL
                     PLRNLPVAIPIDETDLVSAWQQALLQLDLDPKTDAYVLALPASLPVRYAAVLTVINAL
                     VDFVARFPNPHPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPL
                     FGGSVVPVTVKSLAFPS"
     gene            complement(43038..44264)
                     /gene="eutH"
                     /locus_tag="ACHKTW_000615"
     CDS             complement(43038..44264)
                     /gene="eutH"
                     /locus_tag="ACHKTW_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0034228 - ethanolamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034229 - ethanolamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HFO6461691.1"
                     /translation="MGINEIIMYIMMFFMLIAAVDRILSQFGGSARFLGKFGKSIEGS
                     GGQFEEGFMAMGALGLAMVGMTALAPVLAHVLGPVIIPVYEMLGANPSMFAGTLLACD
                     MGGFFLAKELAGGDVAAWLYSGLILGSMMGPTIVFSIPVALGIIEPSDRRYLALGVLA
                     GIVTIPIGCIAGGLVAMYSGVQINGQPVEFTFALILMNMIPVIIVAILVALGLKFIPE
                     KMINGFQIFAKFLVALITLGLAAAVVKFLLGWELIPGLDPIFMAPGDKPGEVMRAIEV
                     IGSISCVLLGAYPMVLLLTRWFEKPLMSVGKVLNMNNIAAAGMVATLANNIPMFGMMK
                     QMDTRGKVINCAFAVSAAFALGDHLGFAAANMNAMIFPMIVGKLIGGVTAIGVAMMLV
                     PKEDATATKTEAEAQS"
     gene            complement(44381..45568)
                     /gene="eutG"
                     /locus_tag="ACHKTW_000616"
     CDS             complement(44381..45568)
                     /gene="eutG"
                     /locus_tag="ACHKTW_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization ethanol dehydrogenase
                     EutG"
                     /protein_id="HFO6461692.1"
                     /translation="MQNELQTALFQAFDTLNLQRVKTFSVPPVTLCGPGSVSSCGQQA
                     QTRGLKHLFVMADSFLHQAGMTAGLTRSLAVKGIAMTLWPCPVGEPCITDVCAAVAQL
                     RESGCDGVIAFGGGSVLDAAKAVALLVTNPDSTLAEMSETSVLQPRLPLIAIPTTAGT
                     GSETTNVTVIIDTVSGRKQVLAHASLMPDVAILDAALTEGVPSHVTAMTGIDALTHAI
                     EAYSALNATPFTDSLAIGAIAMIGKSLPKAVGYGHDLAARESMLLASCMAGMAFSSAG
                     LGLCHAMAHQPGAALHIPHGLANAMLLPTVMEFNRMVCRERFSQIGRALRTKKSDDRD
                     AINAVSELIAEVGIGKRLGDVGATSAHYGAWAQAAQEDICLRSNPRTASLEQIVGLYA
                     AAQ"
     gene            complement(45558..46394)
                     /gene="eutJ"
                     /locus_tag="ACHKTW_000617"
     CDS             complement(45558..46394)
                     /gene="eutJ"
                     /locus_tag="ACHKTW_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutJ"
                     /protein_id="HFO6461693.1"
                     /translation="MAHDEQWLTPRLQTAATLCNQTPAATESPLWLGVDLGTCDVVSM
                     VVDRDGQPVAVCLDWADVVRDGIVWDFFGAVTIVRRHLDTLEQQFGRRFSHAATSFPP
                     GTDPRISINVLESAGLEVSHVLDEPTAVADLLQLDNAGVVDIGGGTTGIAIVKKGKVT
                     YSADEATGGHHISLTLAGNRRISLEEAEQYKRGHGEEIWPAVKPVYEKMADIVARHIE
                     GQGITDLWLAGGSCMQPGVAELFRKQFPALQVHLPQHSLFMTPLAIASSGREKAEGLY
                     AK"
     gene            complement(46405..47808)
                     /gene="eutE"
                     /locus_tag="ACHKTW_000618"
     CDS             complement(46405..47808)
                     /gene="eutE"
                     /locus_tag="ACHKTW_000618"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004029 - aldehyde dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0008774 - acetaldehyde dehydrogenase
                     (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HFO6461694.1"
                     /translation="MNQQDIEQVVKAVLLKMQSSDTPSAAVHEMGVFASLDDAVAAAK
                     VAQQGLKSVAMRQLAIAAIREAGEKHARDLAELAVSETGMGRVEDKFAKNVAQARGTP
                     GVECLSPQVLTGDNGLTLIENAPWGVVASVTPSTNPAATVINNAISLIAAGNSVIFAP
                     HPAAKKVSQRAITLLNQAIVAAGGPENLLVTVANPDIETAQRLFKFPGIGLLVVTGGE
                     AVVEAARKHTNKRLIAAGAGNPPVVVDETADLARAAQSIVKGASFDNNIICADEKVLI
                     VVDSVADELMRLMEGQHAVKLTAEQAQQLQPVLLKNIDERGKGTVSRDWVGRDAAKIA
                     AAIGLNVPQETRLLFVETTAEHPFAVTELMMPVLPVVRVANVADAIALAVKLEGGCHH
                     TAAMHSRNIENMNQMANAIDTSIFVKNGPCIAGLGLGGEGWTTMTITTPTGEGVTSAR
                     TFVRLRRCVLVDAFRIV"
     gene            complement(47820..48107)
                     /gene="eutN"
                     /locus_tag="ACHKTW_000619"
     CDS             complement(47820..48107)
                     /gene="eutN"
                     /locus_tag="ACHKTW_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutN"
                     /protein_id="HFO6461695.1"
                     /translation="MKLAVVTGQIVCTVRHHGLAHDKLLMVEMIDPQGNPDGQCAVAI
                     DNIGAGTGEWVLLVSGSSARQAHKSETSPVDLCVIGIVDEVVSGGQVIFHK"
     gene            complement(48214..48507)
                     /gene="eutM"
                     /locus_tag="ACHKTW_000620"
     CDS             complement(48214..48507)
                     /gene="eutM"
                     /locus_tag="ACHKTW_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HFO6461696.1"
                     /translation="MEALGMIETRGLVALIEASDAMVKAARVKLVGVKQIGGGLCTAM
                     VRGDVAACKAATDAGAAAAQRIGELVSVHVIPRPHGDLEEVFPIGLKGDSSNL"
     gene            complement(48546..49562)
                     /gene="pta"
                     /locus_tag="ACHKTW_000621"
     CDS             complement(48546..49562)
                     /gene="pta"
                     /locus_tag="ACHKTW_000621"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFO6461697.1"
                     /translation="MIIERCRELALRAPARVVFPDALDQRVLKAAQYLHQQGLATPIL
                     VANPFELRQFALSHGVAMDGLQVIDPHGNLAMREEFAHRWLARAGEKTPPDALEKLTD
                     PLMFAAAMVSAGKADVCIAGNLSSTANVLRAGLRIIGLQPGCKTLSSIFLMLPQYSGP
                     ALGFADCSVVPQPTAAQLADIALASAETWRAITGEEPRVAMLSFSSNGSARHPCVANV
                     QQATEIVRERAPKLVVDGELQFDAAFVPEVAAQKAPASPLQGKANVMVFPSLEAGNIG
                     YKIAQRLGGYRAVGPLIQGLAAPMHDLSRGCSVQEIIELALVAAVPRQTEVNRESSLQ
                     TLVE"
     gene            complement(49559..50362)
                     /gene="eutT"
                     /locus_tag="ACHKTW_000622"
     CDS             complement(49559..50362)
                     /gene="eutT"
                     /locus_tag="ACHKTW_000622"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006580 - ethanolamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization cob(I)yrinic acid
                     a,c-diamide adenosyltransferase EutT"
                     /protein_id="HFO6461698.1"
                     /translation="MKDFITEAWLRANHTLSEGAEIHLPADSRLTPSARELLESRHLR
                     IKFIDEQGRLFVDDEQQQPQPVHGLTSSDEHPQACCELCRQPVAKKPDTLTHLSAEKM
                     VAKSDPRLGFRAVLDSTIALAVWLQIELAEPWQPWLADIRSRLGNIMRADALGEPLGC
                     QAIVGLSDEDLHRLSHQPLRYLDHDHLVPEASHGRDAALLNLLRTKVRETETVAAQVF
                     ITRSFEVLRPDILQALNRLSSTVYVMMILSVTKQPLTVKQIQQRLGETQ"
     gene            complement(50359..51060)
                     /gene="eutQ"
                     /locus_tag="ACHKTW_000623"
     CDS             complement(50359..51060)
                     /gene="eutQ"
                     /locus_tag="ACHKTW_000623"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutQ"
                     /protein_id="HFO6461699.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEAREVADLLGFT
                     ITECDESIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLEQGAMQPSFKSVTGKGGIKVIDGSSVKFGRFDGAEPHCVGLTDLVTGDDGSSMAAG
                     FMQWENAFFPWTLNYDEIDMVLEGELHVRHEGETMIAKAGDVMFIPKGSSIEFGTTSS
                     VKFLYVAWPANWQSL"
     gene            complement(51035..51514)
                     /gene="eutP"
                     /locus_tag="ACHKTW_000624"
     CDS             complement(51035..51514)
                     /gene="eutP"
                     /locus_tag="ACHKTW_000624"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HFO6461700.1"
                     /translation="MKRIAFVGSVGAGKTTLFNALQGNYTLARKTQAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            complement(51527..51862)
                     /gene="eutS"
                     /locus_tag="ACHKTW_000625"
     CDS             complement(51527..51862)
                     /gene="eutS"
                     /locus_tag="ACHKTW_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HFO6461701.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIAGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            complement(52155..54434)
                     /gene="maeB"
                     /locus_tag="ACHKTW_000626"
     CDS             complement(52155..54434)
                     /gene="maeB"
                     /locus_tag="ACHKTW_000626"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HFO6461702.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGDQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRDRAPDLMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            54723..55673
                     /gene="tal"
                     /locus_tag="ACHKTW_000627"
     CDS             54723..55673
                     /gene="tal"
                     /locus_tag="ACHKTW_000627"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFO6461703.1"
                     /translation="MNELDGIKQFTTVVADSGDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGRVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     AQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            55693..57696
                     /gene="tkt"
                     /locus_tag="ACHKTW_000628"
     CDS             55693..57696
                     /gene="tkt"
                     /locus_tag="ACHKTW_000628"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFO6461704.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKEIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQLAEEFTRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQVEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(57792..58835)
                     /gene="ypfG"
                     /locus_tag="ACHKTW_000629"
     CDS             complement(57792..58835)
                     /gene="ypfG"
                     /locus_tag="ACHKTW_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HFO6461705.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAAPAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQMIQEGKAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDAWPTLWITR"
     gene            complement(58961..59536)
                     /gene="nudK"
                     /locus_tag="ACHKTW_000630"
     CDS             complement(58961..59536)
                     /gene="nudK"
                     /locus_tag="ACHKTW_000630"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HFO6461706.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKSVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQTSHLMD"
     gene            complement(59604..61583)
                     /gene="aegA"
                     /locus_tag="ACHKTW_000631"
     CDS             complement(59604..61583)
                     /gene="aegA"
                     /locus_tag="ACHKTW_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HFO6461707.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDVALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKCGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHQTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVGVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDSLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRPVPVEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIINWLGVKSVKSH"
     gene            61789..63489
                     /gene="narQ"
                     /locus_tag="ACHKTW_000632"
     CDS             61789..63489
                     /gene="narQ"
                     /locus_tag="ACHKTW_000632"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
                     /protein_id="HFO6461708.1"
                     /translation="MIVKRPVSASLARAFFYIVLLSILSTGIALLTLASSLRDAEAIN
                     IAGSLRMQSYRLGYDLQSGSPQLNAHRQLFQQALHSPVLTNLNVWYVPEAVKTRYAHL
                     NANWLEMNNRLSKGDLPWYQANINNYVNQIDLFVLALQHYAERKMLLVVAISLAGGIG
                     IFTLVFFTLRRIRHQVVAPLNQLVTASQRIEHGQFDSPPLDTSLPNELGLLAKTFNQM
                     SSELHKLYLSLEASVEEKTRDLHEAKRRLEVLYQCSQALNTSQIDVHCFRHILQIVRD
                     NEAAEYLELNVGENWRISEGQPNPELPMQILPVTMQETVYGELHWQNSHVSSSEPLLN
                     SVSSMLGRGLYFNQAQKHFQQLLLMEERATIARELHDSLAQVLSYLRIQLTLLKRSIP
                     EDNATAQSIMADFSQALNDAYRQLRELLTTFRLTLQQADLPSALREMLDTLQNQTSAK
                     LTLDCRLPTLALDAQMQVHLLQIIREAVLNAMKHANASEIAVSCVTAPDGNHTVYIRD
                     NGIGIGEPKEPEGHYGLNIMRERAERLGGTLTFSQPSGGGTLVSISFRSAEGEESQLM
                     "
     gene            63653..66766
                     /gene="acrD"
                     /locus_tag="ACHKTW_000633"
                     /pseudo
     CDS             63653..66766
                     /gene="acrD"
                     /locus_tag="ACHKTW_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416965.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
     gene            complement(66865..66924)
                     /gene="ypfM"
                     /locus_tag="ACHKTW_000634"
     CDS             complement(66865..66924)
                     /gene="ypfM"
                     /locus_tag="ACHKTW_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HFO6461709.1"
                     /translation="MIERELGNWKDFIEVMLRK"
BASE COUNT        15961 a        18393 c        17003 g        15779 t
ORIGIN      
        1 tccccggctt ttttacgcct gttaatcagc taatggctgc gggcgaccaa tcaaataccc
       61 ttgcagatat tgcaccccga gcttatgcaa tagcgcctgc tgctgttgcg tctcgacaaa
      121 ctccgcgacc acactcaatg acttcgcttt cgccagatcg gtaattgatc tcacaatcat
      181 cgcatccagc gtgttcgtga caatatcttt cacaaagacg ccatcaattt tgatgatatc
      241 agcctgcaaa cgctttaacc gttcgtagtt ggcatatccg gtgccaaagt catcaatcgc
      301 aatccggaaa ccaaacttat gcagctgctc gatgttgtac atgctgcttt ctgcgttaga
      361 aaacgcctgc tcctcggtga tctcaagaat gaccgactgc ggggagatgt gataacgctt
      421 aaacagacga ataatccgcc cggcaatatt cttttgcagc agcgtgagcg gcattaaatt
      481 gactgaaaag cgcggtcctt ttttgtcgca agggtgtgtt gccagccact tcaacaagga
      541 ttccagcact tgcaaatcaa aacgcgcgct aaggttaaac tgagcaataa ggggcagaaa
      601 cttatccggg gtcataatgc cgccgtcata tttcagtcgc gcgaggatct catcataacc
      661 ttcaccctct ttgttgcgaa ttggctgggc gtagagcagc aaatcaccct gatctaacgc
      721 cgtgcgaatg gtattcagca atagcacctg tttagtggtc tgcccggaaa ccatctcctc
      781 tctgctatcc agcgccagca catgatgatg tgcgcaggat tgctccgcca gccagcttaa
      841 ctgccccaac aagggttgca gggtttcctg atttccatca aaacgccccc aggcagcacc
      901 atagcccata tccagcccgg tattgttcca gtgaatttgc cgactattca ggatgttaac
      961 catatgctgg agtcgccctt ccgtttccgg cccgctcagc actaacagca gttcactacc
     1021 cggcaattga tacaactttt cgttttcctg catcaacggc agcagcgtac ggcaaattga
     1081 gcggatacag tgaacgcgca tcattaagcc gtaatgacga ctcataaact caagattatc
     1141 aatgcgcagg cagcaaaaac tcttgcccgc ctcttgttcc ggcgcttgtt ccaacgcacg
     1201 aaagttgggt aggagcgtta acggatctgt cagcgcctgc aaatgccagc ggcgattaag
     1261 ccattcactg cgatgataaa tgcgcaccat atagagcagg caaacgctaa aggaaatcag
     1321 caccgcaaga ataaatgcca gcgaatattc ggtttccacc ccttgcaaaa agttctggtt
     1381 gtaattcagc aggcaaagcg ttgaaacagc ccaggtgaga tttaaaaacg gatagcgaag
     1441 cttaccgacc ccgagggtaa agatgatgaa gaaaacgggt accaagtaac cggcaataaa
     1501 gtcgttttca taaggtgtgc acaacagaag tagcagcacg cttaacgctg ccagccagct
     1561 taaggtaaat gcgcgtttct ctttgcccaa cgacggagcg atatccctgc gccacaatat
     1621 ctgcgcaaag tggggactta caatcatgcg agtgagatag tagaaaagca tgttgtaaat
     1681 cagcacagcg gtgaaaaggc ttagcaaatc aacgaccgtg aaaatggcat ccgcatcgcc
     1741 gaaaaaggtg gatatcttta gcgggaaatc aaagaagctc cccacaagat acatactgca
     1801 tttgatgccg atcggcgtca ccaaaccaag ccagaacaga cgttgccaga catatcggct
     1861 ggtcaatcca taacgccaac gcgtccccag ctgccagcgt aatattgcgc aggcacacaa
     1921 cacgacaaac gtctggctac cgagcatgac cgcagtctgt aaaaaagaga gatgaaagtt
     1981 ccacagattg gtgcaaaaca tcccgcataa aacgggaacg acaccgcgcc agccaaagat
     2041 aaacaagatt gacagcatta cgcacagtgg catccacgcc agaaagatat aactggaatg
     2101 gataatggca agtggcgaaa taaaacggga tagctgaata agtaccacgg tgagcgtaaa
     2161 cgctagtgtg aatatcttga tattttttat caggttatgc tccacaaaca tgagtacact
     2221 actctcactg atcggggttt tcaggcacac aaataataag tttattcgca tcgcgaagca
     2281 aacaggggcc agcctgttgc cgatttttca tccaatgatg ctttcaccag gcggcgctaa
     2341 cccggacgta gactgtatta caaaagcggc aaaaagcaga gacaaaaaac ccccgctttg
     2401 cagcgagggt tggaaatttg gtggagctaa gcgggatcga accgctgacc tcttgcatgc
     2461 catgcaagcg ctctcccagc tgagctatag ccccacgatg cgtttacgta ccaaatttgc
     2521 tgggtgcaaa atttggtgga gctaagcggg atcgaaccgc tgacctcttg catgccatgc
     2581 aagcgctctc ccagctgagc tatagccccg tcacgtaaag cttgtcgagt tgacgggcgg
     2641 catcatatga attccgcccg aatgtgtcaa cggcaaattg caacgtgtag tttcaatcgc
     2701 tgaaaaatca ggcaaatgaa caattttgga aaaccgctcg cattcagtag ttattcatgt
     2761 cacggtttcc tgtaaagtgg tgttataaaa tgaactacta atagacccac atacattcag
     2821 ggaattgtta tgttcaagga gaggatgaca ccagatgaac ttgccagact gaccggctat
     2881 agccgccaga ccattaataa atgggtacgc aaggaaggct ggacaacgtc accaaaacct
     2941 ggcgtccagg gtggcaaagc ccgactggtt cacgtcaatg aacaggttcg tgaatatatt
     3001 cgcaatgctg aacgtccaga aggtcaggga gaagcgcctg ccctttctgg tgatgcgccg
     3061 cttgaagtat tgctggtgac actggcgaaa gagatgacgc cagttgaaca aaaacagttt
     3121 acatccttgc ttctgcggga agggattatc ggattgttac aacgcttagg gattcgcgat
     3181 agcaaataat atgaaaagat tacgcaacaa aatgaccacc gaagaactgg ctgaatgcct
     3241 cggtgtggct aaacaaactg ttaaccgttg gatcagagaa aaaggctgga aaacggaaaa
     3301 atttcccggc gtgaaaggcg gtcgtgccag acttattctg gtcgatacgc aagtttgcga
     3361 gtttattcag aacacgccag ccttccataa cacaccaatg ttgatggaag ctgaagagag
     3421 aattgctgaa tacgctcctg gagcacgcgc cccggcgtat cgacaaatca tcaacgcgat
     3481 agataatatg acagacattg agcaggaaaa agtcgcgcaa tttttgtcac gtgaaggaat
     3541 tcgcaatttc ctcgcccgtc tcgatattga cgaatcagca taaataaaaa acggcaggat
     3601 attatctcct gccgtttatc tttttacacg ctaattactg ctgattttcg cgttcagcaa
     3661 taaaatccag cgctttgttg atacgctcga tactgcgggt cttaccaatt gcgtgaacgg
     3721 taacatccag tgctggagac tgccccgcac cggttacggc gacacgcagc ggcataccaa
     3781 ctttacccat acccacttcc agctcatccg ccgtcgcctg aatagcgtga tgaacgtttt
     3841 cagcggtcca gtcagtaatc gcggccagtt tgtcacgaac cacttccagc ggctgacgcg
     3901 ctaccggacg cagatgtttt ttcgccgcat cggcatcgaa ctcagcaaaa tcttcgtaga
     3961 aataacggca gctctgtgcc atctctttca gcgtcttgca gcgttcgccc agcagtttca
     4021 ccagatcagc cagctgcggg ccgttacggg tatcgatatt ttcctgctca atgtgccact
     4081 gtaagtgagt agcaacatac tccggcggca gcgcgttaat gtagtgatgg ttcagccaca
     4141 gcagcttgtc ggtgttgaac gcactggcag atttgctgac ggcattcaaa gtgaagtatt
     4201 tgatcatctc ttcacgagtg aagatttcct gatcgccatg ggaccagccc agacgcacca
     4261 gatagttcag cagtgcttct ggcaaataac cgtcatcacg atactgcatt acgctgactg
     4321 ccccgtgacg tttggacagt tttttaccgt catcgccatt gatcatagaa acgtgcgcgt
     4381 aaaccggcac cggcgctttc agggccttaa gaatgttgat ctggcgcggc gtgttgttga
     4441 tatggtcttc gccacggata acgtgggtga tttccatatc ccagtcatcg acaaccacac
     4501 agaagttata ggttggggaa ccatcggtac ggcggataat cagatcatcc agctcctggt
     4561 tgctgaactc gatcggacca cggatctgat cgtcaaaaac aacagaacct tcctgcggat
     4621 tagcaaaacg cacgacacac ggttcatcat cagcatgatg ctcatggctg tggcggcagc
     4681 gaccgtcata acgcggcttc tcacctttcg ccatttgctc ttcgcgcagc gcttccaggc
     4741 gctctttaga gcaatagcat ttataagcag tgccctcttc cagcatctga tcgatcaccg
     4801 cattgtagcg atcaaaacgt ttggtctggt agtacggacc ttcatcccac tccaggctca
     4861 gccagttcat gccatccata atggcttcga tagcttccgg cgtggaacgc tcaagatcgg
     4921 tgtcttcaat acgcagcacg aactcaccgc cgtggttacg tgcaaaaagc caggagtaaa
     4981 gagcagtacg cgcgccgcca acgtgcagat agcctgttgg gcttggcgcg aagcgagttt
     5041 tgattttcat gaaatggcct tacgtttaga aagatgccga caaccggcaa atcctggaaa
     5101 aattgagtgg gcgatattct atcactcccg cctgattcct caatgtagtt cgggtttcta
     5161 ccttacgcat tattgttttc gttgacaaat tgcgcaacac ggtctgtttt gcgatcatct
     5221 tgcttaaatt tacgacgaac gaacaatttc tttagaaaat gcgttgactc attttgaact
     5281 ctccctataa tgcgactcca cacagcgggg gtgattagct cagctgggag agcacctccc
     5341 ttacaaggag ggggtcggcg gttcgatccc gtcatcaccc accaactact ttatgtagtc
     5401 tccgccgtgt agcaagaaat tgagaagtgg gtgattagct cagctgggag agcacctccc
     5461 ttacaaggag ggggtcggcg gttcgatccc gtcattaccc accactttct cgccagctaa
     5521 atttcttgta aaaatgtgaa gtacctaagt gggtgattag ctcagctggg agagcacctc
     5581 ccttacaagg agggggtcgg cggttcgatc ccgtcatcac ccaccacttc gggtcgttag
     5641 ctcagttggt agagcagttg acttttaatc aattggtcgc aggttcgaat cctgcacgac
     5701 ccaccaatgt aaaaaagcgc cctaaaggcg cttttttgct atctgcgatt tgcgaaattg
     5761 cctgatgcgc ttcacttagc agacgactat ttccggcaat tcctgtctcc tcacctactg
     5821 tgtcaatgca gccaacagct taaccatcgc gggcgtcacc tgctgtgttt cataaacaat
     5881 atataaatct gcagggatgc gctgtttgag cggacggaaa atgacacctg gccagttcat
     5941 ttgtgcgtag ctgtccgcta tcaatgtgat accaataccc atactgacca tggcgagtac
     6001 cgtttgcggt tcattaactt cgcgaataac aaccggtgaa aatcccacct gctggcaaac
     6061 tcgctgcaaa aaatcccagt cagtgtacac gggcggcatt gtaacaaaat actcgtcacg
     6121 tagcgcttcc agcggaacgg tggaaaatga tgagagatga tgctcttcag gcatcgccac
     6181 cagaaacgcc gattcatgca accgtaagct ggtaaaacct gtcggtggtt ctgtcgccat
     6241 tcgccagatc ccggcatcga gttcacgtcg ttccagcagg gccatttgca tcgcgggcat
     6301 cttttcgcga aaaagaactt caacgttagg attttccctg aggaatcgcc gcataaccgg
     6361 gcgcatccgt ccccacatag ccgttcccac tacgccgagt tcaatccgcc ctgcttctcc
     6421 ccgacctatt tgttcaaccc gagccaatac attattagca ttcaccagca atcgacgcga
     6481 ttcttccatc aagattttgc ccgcgtgtgt cagtacgacg ctgcgcgaat ggcgaataaa
     6541 aagctgcgtg ccgagttgat tttccagctc tttaatatga atactgagcg gaggctgaga
     6601 catatttaaa cgcgctgctg cgcggccaaa atgcaactct tccgctacgg caagaaaata
     6661 acggagcaac ttaagatctg ttctgtatac gcgttccata attaagtgct ccatataata
     6721 aatacatcgt attaaattat catggcataa agtatttcac caccagatat ccgacataca
     6781 taaacacaat ggataatata cctggtaata catttggtac aggaataggc aagcgcacga
     6841 ttgtaaacaa agcaccaatg ccaaaaccaa cgccggaaga aaatgaaata tctttcacta
     6901 attaaccttt atcataaaag cagctctgaa gagcagagct gcgaatcctt ttaatgagcc
     6961 accgctcgat gctttatctt ttcagggtca tgattatatt taaacccaaa gaaaaatatc
     7021 actgcgagaa aaagagcata tcctgcaaac accagccaga tagtttgcca gtcttttacg
     7081 ccatccaccg aaaagtaatc tactgccatg ccactcagaa tcgagccaac ccatgcgccg
     7141 acaccattta ccatggtcat aaagagcccc tgcgcgctgg cacgaatgct ggaatcaact
     7201 tcctgttcga caaataccga accagaaata ttgaagaaat cgaatgcaca gccataaaca
     7261 atcatcgaca gcagcagcaa aataaatccg gttgttgacg gatcgccata ggcgaagaag
     7321 ccaaagcgca gcgtccaggc caccatactc atcagcatga cggctttaat gccaaatcgc
     7381 tttaaaaaga atgggatagt cagtataaag cccacttctg ccatctgtga aactgacagt
     7441 aaaatggagg gatatttcac cacaaaactg tcagcaaact ccgggttacg ggcgaaatca
     7501 tgtaggaacg gattaccaaa aacgttggta atttgcagta ccgcacccag catcatggca
     7561 aagaggaaaa agatggccat gcgtggattt ttaaacagca cgaaggcatc cagacccagc
     7621 ttgctggcaa gcgatgtggt cgcttttttc tccgcaaccg gaatcttcgg caaagtcagc
     7681 gcataagccg acagcagcaa tgacgcaccg gacgcgatat acagctgcag actactcaat
     7741 tccagatgca gcaggcttac tgcccacatc gcgacaatga accccaccgt accaaaaacg
     7801 cgaatgggcg ggaaagcggt caccgggtca agccctgcct gggcaagaca ggaataagag
     7861 acgctgttcg ataacgcaat agtcggcata aacgccatcg cattgactaa catcacccaa
     7921 aacatcatat ccggatcagt tacggatgcc gcataaaaaa gtacgcccgc acacaccagg
     7981 tgacacagca tgtatgcacg ttctgcgcgc agccatttgt ctgcgatgat ccccattata
     8041 ccaggcataa taatcgcggc gatccctttg gaactgtaaa ccatgccaac attagcgccg
     8101 gtgaaatgaa gggtattaat catgtaagag ccgagggtaa ccagccagct cccccagata
     8161 aaatattgca aaaaggacat tacctttaag cgcatcgcga tgctcataat cgtttccttg
     8221 tcgcatcatt tggcctcacc cgcgaggccg tttcttattg tttttctcag gcaattttgc
     8281 gcagaaagcc acaaataagg ttgatgaagt tctgcttaga gagctccgca gccgccagcg
     8341 tttgcgcatg tgacaatttc acatcgctta aaccttccgc catattggta atcgcagaga
     8401 ccgcaacgac tttaaggtcg caatggcgag ctgaaataac ctcaggcacc acagacatac
     8461 caacaacatc cccaccaata atttgcatca tgcgaatttc cgccgcagtc tcgaaattcg
     8521 gccccggata cgagacgaac acgccctccg tcagagggaa accctcttct ttcgccactt
     8581 tttgtaacag tgcgcggtat tccgcatcgt aggcatttgc cagtgagaag aagcgctctc
     8641 caaaacgatc atcgttaaga cccaccatcg gcgtaccggg catggtgttg atatgatctt
     8701 tcaatgcgac caggctgcct gcccccactt ccggacgcag cgagcctgcc gcattggtgc
     8761 agaacagtag ctcgcagccc agcagcttaa aggtacgaat tgcgtcagtc ataatggtca
     8821 ttccacgacc ttcgtagaaa tgtccgcgac ctttcataca taccaccggt acgccctgga
     8881 gatggcccag cactaactcc cccgcatgac catgtaccgt acttaccggg aagcccggca
     8941 gcttttcgta ggaaattgcg acagcgttct caatctgatc ggccagcgcg ccgagcccgg
     9001 aacctaaaat aaaggccact cgtggcgtga aatcaggttt ataagtcttg ataatatcta
     9061 tgcaaaacag cgggttatga gaaaattgaa cctgagacat acatatcctt tttctgtagg
     9121 gtggaatcta acgccttttt atagcgaggt tgtcccggct cttaccaata cacttttctc
     9181 acgcattcaa tagcgattcg atatcgatgg atttatatct tttctctatt aatccacggt
     9241 aaaaactgta ttggcgatat taattccttt gcattaacaa tatgtcgttt ttagcaaacc
     9301 actttattaa gcaatctact aataacgatc ggcataggga attatgctat gaaaaaacat
     9361 cttttaactc tgacactttc ctctatatta gcgatacctg ttgtatctca tgcacaattt
     9421 aaaggcggtt ttgcagatat cggcgtgcat tatctggact ggaccagccg gactaccgaa
     9481 aaatcgtcga ccaaatcaca caaagatgat tttggttatc ttgaatttga aggtggcgct
     9541 aactttagtt ggggagaaat gtacgggttc ttcgactggg aaaacttcta taacggtcgc
     9601 cataataaac caggtagcga gcagcgttat acctttaaaa ataccaaccg tatttacctg
     9661 ggtgacaccg gatttaatct ctacttgcat gcgtatggca cctacggttc tgcgaatcgc
     9721 gtgaatttcc acgacgatat gttcctgtac ggtatcggct acaatttcac cggcagcggt
     9781 tggtggttca aaccgttctt tgctaagcgt tatacagatc aaacctatta cactggcgac
     9841 aacggctatg tcgccggctg ggttgcaggt tacaacttta tgctgggcag cgagaaattc
     9901 actcttacca actggaacga gtacgagttt gaccgtgacg ctacctatgc agcgggtaat
     9961 ggcggtaaag aagggctgaa tggcgcagtt gcactgtggt ggaatgcaac atcacacatt
    10021 actacgggga ttcagtatcg atatgcggat gacaaactgg gcgaagattt ttaccaggat
    10081 gcgataatct actccatcaa atttaacttc tgattgatta ctgccggggc tcccggcagt
    10141 atgaattcac tctatatctg atacaacgga tccccttccc gaccagccat tagcgcattg
    10201 ccctggtctc gtaccacgtc ccataacgct tcagcggcgg tggaaagtga acggttcttc
    10261 ctgcgcacca gcatcaactg gcgttcaaca accggcgtaa tgcgctttac caccagtggg
    10321 ctaccttcag gcaatggtag cgccagtgcg ggaagaatac tgatgccgat gcctgcggct
    10381 accatcggga acagcgtcgc cggatgtcca atctcctgca caatattcgc ctgaatacca
    10441 ttgcgcgcca gcgctgcgtc aatcagcggt cggctgcctg acgcgtaatc ctgcaacacc
    10501 aatttcgctc cctgtaatgc ctgccagggt acataatctt cgacggccaa agcgctatcg
    10561 cgatggcaaa gcagaaagaa aggctcggaa agaatcgctt cacattgcag atcgcccact
    10621 gggcctggat cgatgacgat gccaaagtcg acatctccct ggcgaatact ttccatcacc
    10681 cactgctgcg gacgatcgtg caggacaaac tgaatatctg gatagcggcg atggctttca
    10741 gcaatgcatt gcggaataag atgcgcggaa atggtctggc tggcagcgac ccgaactttt
    10801 ccgctcagtt gttgccccat acgcccggta tcgcgcaacg tgctgttcag ttcatccagc
    10861 agtcgctcaa gacgcaaagc cagctgctgc cctgcatctg taagcaccac ttcacgcgtg
    10921 gttctgtcca gcaggcgaac accagtatga ttttccagtt ccttcacact gtgactcact
    10981 gccgactggc tcaggccgat acgctctcct gcacgactaa aacttttctc ctgcgctact
    11041 gtgacgaaaa cctttaattg ttttaaagaa taattcatct gttttcttca tgaatggatg
    11101 caataaatca attttatttc tcaaactgga agaagcacaa tagaaccatc gatcatctgg
    11161 agtctttatg aaactttttc gtatcctcga tcctttcacc ttaaccctga tcacggtggt
    11221 gttgctggcc tctttctttc cggccagagg cgatttcgtc cccttctttg aaaatctgac
    11281 caccgcagcg attgccctgc tgttctttat gcacggcgcg aagttgtcgc gtgaggcgat
    11341 tattgctggc ggtggtcact ggcgactgca tttgtgggta atgtgcagca ccttcgtgct
    11401 gtttccgatt ctgggtgtac tgtttgcctg gtggaaaccg gtaaatgtcg acccgatgct
    11461 ctactccggt tttctctact tgtgcattct cccggctacc gtgcagtctg caatcgcctt
    11521 cacgtcaatg gcgggcggta acgtcgcggc ggcggtttgt tctgcgtcag catccagcct
    11581 gctggggatt ttcctttcac cattgctggt tggtctggtg atgaatgttc acggtgcagg
    11641 gggcagcctt gagcaggtcg gtaaaattat gctgcaactg ctgctgccgt ttgtgttggg
    11701 gcatctttcc cggccgtgga ttggtgactg ggtgtcgcgc aataaaaaat ggattgcgaa
    11761 aactgaccag acgtccattc tgttggtggt ttatacagcg ttcagcgaag ccgtcgttaa
    11821 tggtatctgg cataaagttg gctggggatc attgctgttt atcgtggtgg tcagctgcgt
    11881 tcttctggct atcgtgattg tagttaacgt ctttatggca cgccgactgg gcttcaataa
    11941 ggcagatgaa attactatcg tcttttgtgg ttcgaaaaag agtctggcaa atggcatccc
    12001 gatggcaaac attctgttcc ccacatcggt gatcggtatg atggtgctgc ccctgatgat
    12061 tttccatcag atccaattga tggtctgtgc ggtgctggcg cgtcgataca aacgccagac
    12121 cgaacagtta caggcgcagc aggaaagcag caccgataaa gcttaaagcg gacgcttcag
    12181 gggctggacc agttgcgtca gcccctcggt tttaatcagc agcgtgattt gcattagctc
    12241 accgagcttc cctgccggga actcatcttt gcgggcaaac cacagcagat actcctccgg
    12301 caggtcgatt aagcgacgcc ctttgtattt gccaaacggc attatcgtat tggctatttc
    12361 aatcagctgc tctttttcca tctcagctac ccagcaaacg cagcatttcc gcttcgtcga
    12421 tgacttcaat gcccagttcc tgcgccttcg ccagtttaga tcctgcagct tcacccgcta
    12481 tcaccagatc ggttttcttc gacacgctgc ccgcgacttt cgcgcccagt tcgaccagtc
    12541 gagctttagc gtcatcacgt gacatctgac ttaagctgcc cgtaagcacc acggttttac
    12601 cagcaaacgg gctgtcaatc tcttccgcgt tgataacgat cggcgcaggc cagtggacac
    12661 cttccgccaa cagctcgctg ataacattgc ggttgctttc ttcggcaaag aagttgtgaa
    12721 cgtgggatgc aacgacaatg ccaacatcag gcaccttttg cagctcttca atcgaagcgg
    12781 cttccagcgc ttccagcgtg ccgaaatatg ccgccagacc tgctgcggtg gcctcgccga
    12841 cttcacggat gccaagtgca tagaggaagc gagcaaaggt ggtttctttc gctttttcta
    12901 gcgcgttaac cacgttttgt gccgattttg gccccatacg ctccagtccg gtcagtttgc
    12961 ctgcggtgag tttgaacaga tctgccggag tatggacata ttctttttca accagctgat
    13021 cgatgatttt gtcgcccatt ccgtcaacat ccatcgcacg gcgggaaaca aagtgtttca
    13081 gcgactcttt acgctgcgca ccgcaaatca ggccaccggt acagcgggca accgcttcac
    13141 cttccacacg ctcaacgtca gaaccacata ccggacaatg cgttgggaat acaacctcac
    13201 gggtatcttc cgggcgttca gaaagcacga cgttaaccac ctgcgggatc acgtcgccag
    13261 cgcggcgaat caccacttta tcgccaatgc gtaaaccaag acgttcgatt tcatccgcat
    13321 tgtgtaaggt tgcgttactc accagcacgc cagcaacatg gacaggttcc agacgcgcaa
    13381 caggcgtaat cgcgccagta cgcccaacct gaaactcgac gtcacgcaca aaggtcatct
    13441 gctcctgcgc cgggaattta aacgctaccg cccagcgcgg ggcacgcgcg acaaagccaa
    13501 gctgctcctg ctgttccagt gagttgacct taatcaccac gccgtcgata tcaaagccca
    13561 gcgtcgggcg gtcttcttcc actttgtgat agaacgccag cacttcttcc gccgattcac
    13621 aaagcgttac ccgatcgctg accggcaacc cccacttttt aaattgcagt aaacggccaa
    13681 gatgagtatc cggcagctcg ccaccttcca gaacaccaac gccatagcag aaaaaagtga
    13741 gcggtcgctt cgctgtaata cgcggatcaa gctgacgcag tgaaccagct gccgcattac
    13801 gtgggttagc aaaaactttc ccgcccgtgc gtcgcgcatc ttcgttaatc ttttcgaacc
    13861 ccgcctgcgg caggaacact tcaccacgca cttccagacg cgccgggata ttctctccgt
    13921 gcagcttcag cggaatagcg cgaatagtac gcacattaga cgtgatatct tccccggtgg
    13981 tgccatcgcc acgggtcgcg gcactgacta aaacgccatt ttcatacaga atactgacgg
    14041 caaggccatc cagcttcagc tcacagcacc aggtgacttt ctcgttgttt ttcagacggt
    14101 cctgcacacg tttgttgaaa gcaagaaagc tttcttcatc aaaaacgtta tccagtgaca
    14161 gcattggtac ttcatggcgt atctggctga aagccgccag cggcgcagcg cctacacgtt
    14221 gggtaggcga atcaggcgta atcagttctg gatgtttggt ttccagctcg cgcagttcgc
    14281 gcatcagcct gtcgtattca gcgtcgggaa tttccggcgc atccatcaca tgataaagat
    14341 attcatgatg gcgaagcgtc gttcgcagtt ctgtcagttg ttgttcgatt gattccatat
    14401 cgcaccatca atgctaaaaa cccccgacaa gcgggggttc gaagaggagt taatttgcct
    14461 taggtgtatc aggcgttggc gtctttgact tcgcggatga tgtcctggta ctcgcgcaat
    14521 ttctgcggag tcatcatacg gcgctgatcg tcaagcacga caccgcccac ttcatcggca
    14581 atatgctgcg cagattgcag catcagcttg aagttctgca gctcgtcacc gtaagacggt
    14641 acctgcataa agatggtgac acccggagta gtgaaatcct tcatttcagg atcaaaggtt
    14701 cccggtttca ccatattcgc caggctgaat aacgccgggc cgctgccatc cgggctaaga
    14761 tgacgatggt aaatattcat atcgccaaaa atgaagcccg cttgttgaat gctgttaaga
    14821 agcagttcac cgtttagctc gctaccgtga tgcgccgcga cgttcatgat aatcaccgct
    14881 tctttgcgct tcggtttatc cgtaactgga gccggttccg ctacaggctc aggctgtggt
    14941 gccgctacgg gttctgcagg ctggaaagcc tgttgtgccg gttgcggtgc tgaatgcaca
    15001 ggctgcggcg ctggcgcgac ctgtggcgaa actggctgct gcaacggctg ttcaggctgc
    15061 ggctgatagg caggctgctg caccggctgc gctggacgcg gagcatgttg cggcggtacc
    15121 tgcgcttcag gcggctgctg gaccggttga cgcggctgcg cagacgcata aggcggttgg
    15181 tactggtgtt gcggcgacgg acgagcagcc tcatgctcct gagcgttagc cggggcatga
    15241 ttcacgcggt gaacacgaac ctcaccaacg ccctcatcat cttcgacatc ctcgtcataa
    15301 gaatcgtcgt cacgttttga cttcattcgt tttaatggcc gatcgcggaa catagaagat
    15361 cgttctttac ggctggtcca gaaaccatgt accagtaaag cgattatggc gatcgcgcca
    15421 acaatgatta atatcagacg caaatcctgc atcattatat tctctgttgt tctaacacct
    15481 tgccaccacg gcaaacattt actcactaag agtatttgcc gattacctca agtgcaagtg
    15541 cactattaac tttcacagca caaagataga tgaaatcgtg ctttttgctg ttttttcgaa
    15601 catatcctaa ctgtccattg cgcaattacc cggtaaaata cgcagaattt tcctgggatt
    15661 ggtcaaaagg agctcatcct gattatggtt tcatcattca catctgcccc acgcagcggt
    15721 ttttactatt ttgcgcaagg ctggaagctc gtctcgcaac ctgggattcg gcgtttcgtt
    15781 attttaccgc tgctggtcaa tattttgttg atggggggcg cattctggtg gctctttaca
    15841 cagctcgatg tctggatccc gactctcatg agttacgttc cggactggct acaatggctg
    15901 agttatctgt tgtggcctct ggctgtcatc tctgtgctgt tagtgtttgg ctatttcttc
    15961 tccacgattg ctaactggat tgccgctccg tttaacggtt tattggctga acaactggaa
    16021 gcacgattga ctggcgctac accgccagat accgggattt tcggtatcat gaaagatgtg
    16081 ccgcgaatca tgaaacgcga atggcaaaaa tttgcctggt atctgccgcg cgcaattgta
    16141 ttgctaattc tttacttaat ccctggtatt gggcaaaccg tcgcgccggt actgtggttc
    16201 ctgtttagcg cctggatgtt agccatccag tattgcgatt accccttcga taaccacaaa
    16261 gtgccgttta aagagatgcg caccgccctg cgcacacgca aaatcaccaa tatgcagttt
    16321 ggtgctttaa ccagcctgtt tacgatgatc ccgctgctta atctgttcat catgcccgtt
    16381 gccgtttgtg gcgcgacggc gatgtgggtc gattgctatc gcgataaaca cgcgatgtgg
    16441 cggtaacaat ctaccggtta ttttgtaaac cgtttgtgtg aaacaggggt ggcttatgcc
    16501 gccccttatt ccatcttgca tgtcattatt tcccttctgt atatagatat gctaaatcct
    16561 tacttccgca tattctctga gcgggtatgc tacctgttgt atcccaattt catacagtta
    16621 aggacaggcc atgagtaaga tttttgaaga taactcgctg actatcggtc acacgccgct
    16681 ggttcgcctg aatcgcatcg gtaacggacg cattctggcg aaggtggaat ctcgtaaccc
    16741 cagcttcagc gttaagtgcc gtatcggtgc caacatgatt tgggatgccg aaaagcgcgg
    16801 cgtgctgaaa ccaggcgttg aactggttga accaaccagc ggtaataccg ggattgcact
    16861 ggcctatgta gctgccgctc gcggttacaa actcaccctg accatgccag aaaccatgag
    16921 tattgaacgc cgcaagctgc tgaaagcgtt aggtgcaaac ctggtgctga cggaaggtgc
    16981 taaaggcatg aaaggcgcaa tccaaaaagc agaagaaatt gtcgccagca atccagagaa
    17041 atacctgctg ctgcaacaat tcagcaatcc ggcaaaccct gaaattcacg aaaaaaccac
    17101 cggtccggag atctgggaag ataccgacgg tcaggttgat gtatttattg ctggcgttgg
    17161 gactggcggt acgcttactg gcgtcagccg ctacattaaa ggcaccaaag gcaagaccga
    17221 tcttatctct gtcgccgttg agccaaccga ttctccagtt atcgcccagg cactggctgg
    17281 tgaagagatt aaacctggcc cgcataaaat tcagggtatt ggcgctggtt ttatcccagc
    17341 taacctcgat ctcaagctgg tcgataaagt cattggcatc accaatgaag aagcgatttc
    17401 taccgcgcgt cgtctgatgg aagaagaagg tattcttgca ggtatctctt ctggagcagc
    17461 tgttgctgcg gcgttgaaac tacaagaaga tgaaagcttt accaacaaga atattgtggt
    17521 tattctaccg tcatcgggtg agcgttattt aagcaccgca ttgtttgccg atctcttcac
    17581 tgagaaagaa ctgcaacagt aatgccagct tgttaaaaat gcgtaaaaaa gcaccttttt
    17641 aggtgctttt ttgtggcctg cttcaaactt tcgcccctcc tggcattgat tcagcctgtc
    17701 ggaactggta tttaaccaga ctaattattt tgatgcgcga aattaatcgt tacaggaaaa
    17761 gccaaagctg aatcgatttt atgatttggt tcaattcttc ctttagcggc ataatgttta
    17821 atgacgtacg aaacgtcagc ggtcaacacc cgccagcaat ggactgtatt gcgctcttcg
    17881 tgcgtcgcgt ctgttaaaaa ctggcgctaa caatacaggc taaagtcgaa ccgccaggct
    17941 agactttagt tccacaacac taaacctata agttggggaa atacaatgtt ccagcaagaa
    18001 gttaccatta ccgctccgaa cggtctgcac acccgccctg ctgcccagtt tgtaaaagaa
    18061 gctaagggct tcacttctga aattactgtg acttccaacg gcaaaagcgc cagcgcgaaa
    18121 agcctgttta aactgcagac tctgggcctg actcaaggta ccgttgtgac tatctccgca
    18181 gaaggcgaag acgagcagaa agcggttgaa catctggtta aactgatggc ggaactcgag
    18241 taatttcccg ggttctttta aaaatcagtc acaagtaagg tagggttatg atttcaggca
    18301 ttttagcatc cccgggtatc gctttcggta aagctctgct tctgaaagaa gacgaaattg
    18361 tcattgaccg gaaaaaaatt tctgccgacc aggttgatca ggaagttgaa cgttttctga
    18421 gcggtcgtgc caaggcatca gcccagctgg aaacgatcaa aacgaaagct ggtgaaacgt
    18481 tcggtgaaga aaaagaagcc atctttgaag ggcatattat gctgctcgaa gatgaggagc
    18541 tggagcagga aatcatagcc ctgattaaag ataagcacat gacagctgac gcagctgctc
    18601 atgaagttat cgaaggtcag gcttctgccc tggaagagct ggatgatgaa tacctgaaag
    18661 aacgtgcggc tgacgtacgt gatatcggta agcgcctgct gcgcaacatc ctgggcctga
    18721 agattatcga cctgagcgcc attcaggatg aagtcattct ggttgccgct gacctgacgc
    18781 cgtccgaaac cgcacagctg aacctgaaga aggtgctggg tttcatcacc gacgcgggtg
    18841 gccgtacttc ccacacctct atcatggcgc gttctctgga actgcctgct atcgtgggta
    18901 ccggtagcgt cacctctcag gtgaaaaatg acgactatct gattctggat gccgtaaata
    18961 atcaggttta cgtcaatcca accaacgaag ttattgataa aatgcgcgct gttcaggagc
    19021 aagtggcttc tgaaaaagca gagcttgcta aactgaaaga tctgccagct attacgctgg
    19081 acggtcacca ggtagaagtg tgcgctaaca ttggtacggt tcgtgacgtt gaaggtgcag
    19141 agcgtaacgg cgctgaaggc gttggtctgt atcgtactga gttcctgttc atggaccgcg
    19201 acgcgctgcc cactgaagaa gaacagtttg ctgcttacaa agcagtggct gaagcgtgtg
    19261 gctcgcaggc ggttatcgtt cgtaccatgg acatcggcgg cgacaaagag ctgccataca
    19321 tgaacttccc gaaagaagag aacccgttcc tcggctggcg cgctatccgt atcgcgatgg
    19381 atcgtaaaga gatcctgcgc gatcagctcc gcgctatcct gcgtgcctcg gctttcggta
    19441 aattgcgcat tatgttcccg atgatcatct ctgttgaaga agtacgtgca ctgcgcaaag
    19501 agatcgaaat ctacaaacag gaactgcgcg acgaaggtaa agcgtttgac gagtcaattg
    19561 aaatcggcgt aatggtggaa acaccggctg ccgcaacaat tgcacgtcat ttagccaaag
    19621 aagttgattt ctttagtatc ggcaccaatg atttaacgca gtacactctg gcagttgacc
    19681 gtggtaatga tatgatttca cacctttacc aaccaatgtc accgtccgtg ctgaacttga
    19741 tcaagcaagt tattgatgct tctcatgctg aaggcaaatg gactggcatg tgtggtgagc
    19801 ttgctggcga tgaacgtgct acacttctgt tgctggggat gggtctggac gaattctcta
    19861 tgagcgccat ttctatcccg cgcattaaga agattatccg taacacgaac ttcgaagatg
    19921 cgaaggtgtt agcagagcag gctcttgctc aaccgacaac ggacgagtta atgacgctgg
    19981 ttaacaagtt cattgaagaa aaaacaatct gctaatccac gagatgcggc ccaatttact
    20041 gcttaggaga agatcatggg tttgttcgat aaactgaaat ctctggtttc cgacgacaag
    20101 aaggataccg gaactattga gatcattgct ccgctctctg gcgagatcgt caatatcgaa
    20161 gacgtgccgg atgtcgtttt tgcggaaaaa atcgttggtg atggtattgc tatcaaacca
    20221 acgggtaaca aaatggtcgc gcctgtagac ggtaccattg gtaaaatctt tgaaaccaat
    20281 cacgcgttct ctatcgaatc tgatagcggc gttgaactgt tcgtccactt cggtatcgac
    20341 accgttgaac tgaaaggcga aggcttcaag cgtattgctg aagaaggtca gcgcgtgaaa
    20401 gttggcgata ctgtcattga atttgatctg ccgctactgg aagagaaagc caagtctacc
    20461 ctgactccgg ttgttatctc caacatggac gaaatcaaag aactgatcaa actgtccggt
    20521 agcgtaaccg tgggtgaaac cccggttatc cgcatcaaga agtaattctt gccgcagtga
    20581 aaaatggcgc ccatcggcgc cattttttat gcttccgcca gcggcggcaa aatcaattca
    20641 tcgctctcat gctgctgggt gtagcgcatc acttccagta cgcgcaaccc cgcacggtgc
    20701 accgcatcgt ttaacgcctt ccctttcagc aaaccactga taagctgagc acaaaacagg
    20761 tcgccagtcc ctttcaggtc ggtttttacc cgtgaatggg aaatgacatt cacgctgtcg
    20821 gcactgacca ccaccacctg catctcctga ttttcttcat taccggaggc gctggtaatc
    20881 accacccatt ttaatgtgtc tgaaagcaga ctttttgcgg cagcaatggc actgtcgaga
    20941 tcgcggcaat ttttaccggt caggatttcc agctcaaaga tattgggggt aattccctgc
    21001 gccagcggca gtaaatattg tcgatacgcc tcgggaaggt caggtttgac ataaattccg
    21061 ctatcaatat cgccaatcac cggatcgacc atgatcaata ggtcaggatg gtctttgcgt
    21121 agcgcagtca gccactcggc aaggattttg atttgcgatg ccgttcccat atagcccgtg
    21181 gttacagcgc gcagctggcg cagcgcatca cgctcctgaa gcgcacgcaa ataaccgcta
    21241 aaccattcat ccggaatcgc accaccgtag aaagtgtcat aatgcggcgt attgctcagc
    21301 aataccgtcg gcacggcaaa aacattcaga ccgttctgtt tgatagcagg cacggcaatg
    21361 ctgttgccca cgctgccgta aaccacctgc gactgcacgg cgacgatatc cgcctgcagc
    21421 gccctgctct tatcgttaaa caacaacaaa ctactcattt aattttttct ccttgccgat
    21481 gatcctcatc gtattccaac cgaaacttta cctgattctg gcagtcaaat cggctatcac
    21541 aaaacaagga taaggtaatt caatgaagaa aatcatttgt ctggtcatta cactactaat
    21601 gacactcccc gcttacgcga agttaactgc ccatgaagaa gcccgcatca acgccatgct
    21661 ggagggatta gcacagaaaa aggatttgat atttgtgcgc aacggtgatg aacatacctg
    21721 cgatgaagcg gtttctcatc tgcgtctgaa gctgggcaat acccgtaacc gcattgacac
    21781 tgccgagcag tttattgata aggttgcttc gtcgtcatcg attaccggga agccgtatat
    21841 tgtgaagatc cccggtaaga gcgatgagaa cgcacagcct tttttacatg cgctaattgc
    21901 gcagacggat aaaacggtgc ctgcgcaata atcttaaatc cccgccccct ggctaaaatg
    21961 ctcttcccca aacaccccgg tggaaaggta gcgatcgcca cgatcgcaga tgatcgctac
    22021 caccaccgca ccagggttag cttttgccac ccgcagtgct ccggcaaccg cgccgccgga
    22081 gctgacgcca cagaatattc cttcccgcac tgccagttcg cgcatggtgt tttctgcatc
    22141 gcgctgatga atatccagca cctcatccac cagagaagcg ttgaaaatcc ccggcagata
    22201 ttccgcaggc cagcggcgaa tgccgggaat actgctgcct tcttccggtt gcaggccaac
    22261 aatggtcacc ggtttggatt gttcgcgcat aaagcgtgaa acgccggtga tagtgccggt
    22321 cgtccccata ctggagacaa aatgagtgat gcgcccgccg gtttgctgcc agatttccgg
    22381 cccggtggtg gtgtaatgcg cataagggtt atcgggatta ttgaactgat cgagcagctt
    22441 tccttcgcca cgattcgcca tctccagcgc cagatcgcgc gcaccttcca tgccctgctc
    22501 tttggtgaca agaatcagtt ccgcaccata agcacgcatc gccgcacggc gttcctggct
    22561 catgttgtcg ggcatcagca atttcatgcg atagccttta agcgcggcaa tcattgccag
    22621 cgcaatgccg gtgttaccac tggtggcttc gattaagaca tcacccggtt taatttcccc
    22681 gcgcttttcc gcctcgacga tcatcgaaag tgccgcacga tctttcaccg aacctgccgg
    22741 gttattgcct tccagtttta accacacttc actgccgtta tccggcccca ttcgctgcaa
    22801 cttcaccaga ggcgtattgc ctattgtttg ttctaatgta ctcacgatct ctatccatac
    22861 gtggtgttgc ctgatgcgac gcttgcgcgt cttatcaggt ctacaggtta caaaccttgc
    22921 cattaaaaag cccggatcgc gggaagcgcc tccgggcgtt taacattcac tcaacctatc
    22981 aggcgctttg tgcgagagca agttcctcat cgcgggtttc gatacgctcg tcgccgttat
    23041 acagccgcgc atgttgcaga ccaacgaata aacgctcgcc acgctgcggg gcatcgtcgc
    23101 catgcatcac gaccgtcagc ggttcgttgt accaccccag cggctgcacc actaattggg
    23161 tgtagtgacc tttcgggctg gcttccagta cctgtaccgg cagcggcgaa tcgagactgg
    23221 tacggcggct gatatccact tcccacgggc gcaggaagag atccaccggc ccctgataag
    23281 caggtgtgta gcccagcggc cagcgatgtg cgccaacatg gaactgcccg ccgcgaatgg
    23341 ttccctgcag gcggttcact tcgcccataa attcgagtac aaaacgggtc gctggttcgc
    23401 gccatacctg attcggcgca tcagcctgtt caatattccc ctgactcatc accactacac
    23461 gatcggctac ttcggtcgcc tcttcctggt cgtgggtcac aaacacgctg gtgaatttca
    23521 gctcttcatg cagttgacgc agccagcgac gcagctcttt acgcacctgc gcatccagcg
    23581 cgccaaacgg ttcatcaagc agcagaattt gcggttctac agccagcgcg cgcgccagcg
    23641 ccacacgctg tttctggccg ccggaaagct gcgccggata acgatccgcc agatgcgcaa
    23701 gctggaccat ttccagcaat tttgtcactt tcgctttgat ggctgcggca ttcgggcgct
    23761 cgcgacgcgg cagcaccgtc aggccaaaag cgatattgtc gaataccgtc atatggcgga
    23821 acagcgcgta atgctggaac acgaaaccga ccttgcgatc gcgcgcgtgc aggcggctga
    23881 cgtcggtgcc gtggaagcga atatgcccgc tggtttgatg ctccagcccg gcgataatgc
    23941 gcagcagcgt ggttttcccg gaaccggacg gccccagcaa cgcgaccatc tgacctgaag
    24001 gaatatccag tgagatatcg ttcagcacct gggtgcgacc aaacgacttc ttaatattgg
    24061 caatctcaat gctcatgatg ttcctcctgc tgtgcgcgtt tttcctgatt ctccaggcgc
    24121 cactgcaaca tactttttaa aaacagggtg ataatcgcca tcagcgttaa cagtgccgca
    24181 gcggtaaagg agccgacggt gttgtagtcc tgctccagca attcaatctg taacggcagc
    24241 gaaagtgttt cgccgcgaat cgagccggaa accaccgaca ccgcgccaaa ctcgccaatc
    24301 gcgcgggcgt tggtcagcac tacgccataa agcagcgccc agcggatgtt cggcaatgtg
    24361 acacgacgga acatctgcca gccagacgcg ccaagcaaaa tcgccgcctc gtcttcctga
    24421 ctgccctggc ttaacatcac cggcaccagt tcgcgcacca caaacggaca cgtcacgaag
    24481 atggtgacca gcaccattcc cggccaggag aacataattt gcagattatg ttcatccagc
    24541 caaccgccca gcgggccgtt agagccgtag aacagcagat acaccagacc ggcaaccacc
    24601 ggcgatacgg caaacggaat gtccagtagc gtcagcagta actggcgtcc agggaagtta
    24661 aagcgcgtca ccagccaggc cagcagaatg ccgaacacca ggtttaccgg tacggcaatc
    24721 agcgcgatca tcaccgtcag ccagatggcg tgtagcatgt ccggatcggc cagattctgt
    24781 aaaaccggca tcagcccttt gctgaacgcc tgtacgaaga tataaatcat cggcaccagc
    24841 aggatgaacg ccgataccag catcccgatg ccaatcagaa accatttgcc ccagttaatc
    24901 gggcgcgcgt cataacgctt caattgggta acttccgcca ttaatgacct accacacgcc
    24961 gaccaaagcg actttgcaga gtgttaattg agaacagcag cagcagagat gccgcgagga
    25021 tcaccgaagc aatcgcgctc gctgccgggt aatcaaactc ctgtaagcgc acaaaaatca
    25081 tcagcgacgt cacttccgtc ttccacgcga tatttccggc gataaaaatc accgcgccaa
    25141 attcaccaag actacgggta aacgacagcg ccacgcccgc caccagcgcc ggagaaagct
    25201 ccggcagcac cactttgcag aaactctgcc agcgcgttgc accaagcgtt tccgccgctt
    25261 cttcatattc cgggcctaac tcttccagca ccggctgcac ggtacgtacc acaaacggaa
    25321 tgctggtaaa ggccatcgcc accgcaatcc ccagccaggt ataggtgact ttgatatcaa
    25381 acttcgccag ccattcaccg taaaaaccgt ttacagaaaa gagcgaggct agcgttaaac
    25441 cggcgactgc cgttggcagc gcaaagggta aatccatcag cgcatcaagc agcgtgcggc
    25501 ctgggaagcg atagcgggtt aggatccacg ccatcagcag accgaaaacg ccgttaaaaa
    25561 tcgatgccac aaacgctgac agcagcgtta ctttgtaggc cgctactacc tgcgggttgg
    25621 tgatcacctc ccagtactgc gcccagctca tctgagccag ctgcatcacc agcgcggaga
    25681 gcggcagcag caaaatcagg cacacaaaca gcagactggt gccgaggctt aaggtaaagc
    25741 ccggcagcac gcgtcttgat gaaactgcaa acattaatta cgccccgccg ctaacagctt
    25801 gtctaactcg ccgccgctgg tgaagtgggt tttcatcact tccggccagg agccaaattt
    25861 gtcttccacg cggaacagct cggtctgcgg gaatttgttt ttcagtttgt ccatgacttc
    25921 cgggttattc acacggtagt aatagtcggt gatgatggtc tgcgcctgtg ggctgtagag
    25981 ccagttcagg taggcttttg cggctttttc cgtaccgttg gcctgcacat ttttatcgac
    26041 ccacgccacc gggaattccg ccagaatatt ggttttcggg atcaccactt caaagccctg
    26101 tgcttcatac tgtttacgga tgttgttcac ttccgattca aagctaatca gcacatcgcc
    26161 caggccacgc tcggcaaaag tggtggttgc gccacgaccg ccggtatcga acacttcaac
    26221 gtttttcagg aactgggtca taaactgttc ggttttggct ttgtcaccac cgtcagcttt
    26281 atccgctgcg ccccatgccg ccagataggt ataacgcgcg ttacccgacg ttttcgggtt
    26341 cgggaaaatc agcttcacgt cggagcgcac caggtcgttc caatcgtgga tattcttcgg
    26401 gttaccctta cgcaccagga agcccatggt ggagtagaac ggcgagctat tattcggcag
    26461 gcgcgactgc cagtcggccg ggatcagctt gcctttatcg tgcaggattt gtacgtcggt
    26521 cacctggtta taagtgacaa cgtcggcttt taagccctgt aaaatcgcca gcgcctgttt
    26581 tgatgacccg gcatgagatt gttttatcgt cagtttgtcg ccgccgttat cttttgccca
    26641 ttgttgctca aacggcggat tcagggcggc aaacagctcg cgggagacgt cataagaact
    26701 gttcagcagt tccgttgcct gtacatggcc cgccagcagc agagaagcga ccagcgcgag
    26761 tgagttcttt ttcagtaagt taacggccat tgcgcaccct tataaattta atgactttct
    26821 aatagccatc atatttataa cggacgtgaa aggagtaacg gttttatata ccgtttggtg
    26881 atttggaagt tgaaaaggga ataagaaccg ggtttaaaac gaaatcctcc ccacacatgc
    26941 agggaggaaa cagaggtgaa tcagataccg acgctaaccg tctccggcag tgtgctgccg
    27001 ccatcaatca cattctgtgt accggttaaa tagctggatt catccgatgc gaggaaggcc
    27061 gccagttcgc cgacttccag cggatcggcg aggcgacgca tcgggattgc tttcgccatt
    27121 tcagtcagca ccgactctgg atcttccggg ttcgactggc gggcaatgct ttccgccatt
    27181 ggtgtgcaca cgtatcccgg gcaaatggcg ttaacgcgaa taccagactg cgcgtactcc
    27241 accgccagcg attttgtcag gccaacaatc gccgctttcg ttaaggcgta cgccgtttcg
    27301 ccaggatcgg ccaccatatc gccagtgacc gaagacatca tcacaatgcg accatctttg
    27361 cgggcaatca tctccggcag caccgccttc gtgacgttcc atacgccttt aatattgatg
    27421 tcaatatgga aatcgcgatc gtcatcgctc atatcgagga aactgcccag acgacaaacg
    27481 cctgcgttat tcaccaggat atcaatgcgc ccttcttttt ccttcgcgcg tttgatagct
    27541 gcggctaccg acgccgggtc acgcacatcg gcgacaaccg ccgtacagcg atgaccacga
    27601 ccacacagtt cgtccgccag cttttcgatc tcaggggaga tatccagcaa gattaggttt
    27661 gcgccatgac gcgcaaaagt tctggcaatt ccttcgccaa ttccctgcaa tgcgcccgta
    27721 atcagtgctg tcttgcccgt gagtttaccc attttaatgc tctccttgtt aatacagagc
    27781 gttacactcc attccctctg aaaatacagg ataaacaaaa tattgctgcg gatcagctca
    27841 ctaattcagg aaaatctcaa tccagtcgct gctttatgca tcatttcagg cgttgtgtta
    27901 gctcactgct gcgctgaatc attttcaatg actccacatc attgagttgc acaagaccaa
    27961 caaacagtaa atcggtaaca gaattttgcg ccgtgcgggt agacatcgac gagctacgcc
    28021 attcggtttc accggaaacg gtatcgagcg tgaaatgcgc cagccgccgc agcggagaat
    28081 cagccagaga agtaatggca atcaccgttg ccccctgctt tcgtgccgct tctgcgcata
    28141 agacaatctc ttttttactg ccgctgtaag agatagcgat ttgtacatcg ccttttttca
    28201 acgcctggga aacggtggct tgtacgtgcg tgtcggcttc gcaggccacg cgatatccaa
    28261 ttttcatcag tttgaacgac agatcgcgcc ccaccagcgc cgatccgccc aggcccgtta
    28321 tctgaataaa ttgcgcttta ctgatgacat caatgatttt ttgcagccgc gcgtagtcga
    28381 acaacgcgca ggtttgctcc agcgccagct ctttttcgcg attcagtttg cgggcaatca
    28441 cttccagcga atcatcactg gtaatcgaac tgtgcaggtg cagcgccgtg gcattcgttt
    28501 tctcccggct ggcactgtat tcgccaatta acgccatacg caattcagta aatccttgtg
    28561 cgccgagctt ctgggcaaac ttcacaatac tcgactggct gatgccgagc tgtttcgcca
    28621 tcttgcgcga agagacggat tgcagctcac tcacgttggc ctgcagaaag tcagcgattt
    28681 tttgctcgtt ttcggtgaat tcactccccg cattgcttat tttcgtcagg tacaacatct
    28741 tccactctcc tgggctggca gcataaattc cgcttaagtt cttatgacgc tcttcacact
    28801 ctgcgagtgt agactcaatg attcctttaa gtctgtcaat tcgggatata tgattccata
    28861 catctgcaat tatttgatcg taaatagtaa ggtcaccacc gatgcaacta gaaaagatga
    28921 ttactgaagg ctcgaacacc gcctcggctg aaattgaccg cgtatcgacg ctggaaatgt
    28981 gccggattat caacgatgaa gataaaaccg taccgcttgc cgttgagcgc gtcctgccgg
    29041 atatcgccgc ggcgatcgat gttatccacg cccaggtcag cggcggcggt cgtctgattt
    29101 atctcggtgc gggaacatcc ggtcgtctgg ggattctgga tgccagcgaa tgtccgccca
    29161 cctacggcgt gaaaccgggt ctggtggttg gtttgattgc tggaggcgaa tatgccattc
    29221 agcacgcggt ggaaggcgct gaagatagcc gggaaggtgg tgttaacgat ctgaaaaata
    29281 ttaatttaac ggcacaggat gtggtggttg gcattgctgc cagcggtcgc acgccgtatg
    29341 tgattgccgg actggaatac gcacgccagc tcggctgccg cacagtggga atttcctgta
    29401 atccggggag tgccgtttcc accaccgccg agtttgccat tacaccgatt gtaggtgccg
    29461 aagtggtcac tggttcttcg cgaatgaaag caggcacagc gcagaaactg gtgctcaata
    29521 tgctttccac cgggctgatg attaaatctg gcaaagtgtt cggcaacctg atggtcgatg
    29581 tggtcgccac caacgaaaaa ctgcatgtgc gccaggtcaa tatcgttaaa aacgccaccg
    29641 gatgtaatgc cgaacaagcg gaagcggcgt taattgcctg cgagcgcaac tgtaaaacgg
    29701 ccattgtgat ggtgctgaaa aatctcgatg ccgcagaagc taaaaaacgc ctggatcaac
    29761 acggcggctt tattcgtcag gttttagaca aggaataacc catggccaaa gagatcagca
    29821 gtgaacttct gaacaccatt cttacccgtg tcggcggacc gggaaatatc gccagttgtg
    29881 gtaactgtat gacgcgcctg cgtctgggtg tacatgacag ttcactggtt gatcccaata
    29941 tcaaaacgct ggaaggcgtg aagggcgtca ttttgaccag cgaccaggtg caggtcgttt
    30001 ttgggcctgg taaagcgcat cgtgccgcaa aagcgatgag cgagctgctg ggcgaggcac
    30061 cggtacagga tgccgcagaa atcgccgccc agaacaaacg tcagttaaaa gccaaacaaa
    30121 cctccggcgt gcaacaattt ctcgccaaat tcgccaccat cttcacgccg ctgatccccg
    30181 gttttattgc cgccggtctg ctgctgggga tagcgacgtt aattgccacg gtgatgcacg
    30241 ttccggcaga tgctcagggc acgctacccg acgcgctgaa ttttatgaag gtgttcagca
    30301 aaggtttgtt cactttcctg gtgattctgg tgggctataa cgccgcccag gcatttggcg
    30361 gcacgggcgt caatggcgca attattgccg cactcttttt gctcggttat aaccccgctg
    30421 caaccaccgg ttactacgcc ggttttcacg atttctttgg tctgcccatc gatccgcgcg
    30481 gcaatattat cggcgtgctg attgccgcgt gggcctgcgc ccgcattgaa ggcatggtgc
    30541 gccgctttat gccggacgat ctcgacatgc tgctgacctc gttaatcacc ctgctgatca
    30601 ccgctacgct tgcgtacctg atcattatgc cgctgggtgg ctggttattc gaaggtatgt
    30661 catggctgtt tatgcacctg aacagtaatc cgttcggttg tgcggtttta gccgggctgt
    30721 tcctgatcgc cgtggtattt ggcgtgcatc agggctttat tcctgtttac ctcgcgttaa
    30781 tggacagcca gggattcaac aacttatttc ccatcctttc aatggcaggc gcgggccagg
    30841 tgggcgcggc gctggcactc tactggcggg cgcaaccgca cagtgcatta cgcagtcagg
    30901 tacgcggggc gattattccc ggtctgctgg gcgttggtga accgttgatt tacggcgtca
    30961 ccctgccccg catgaaaccg tttattaccg cctgtttagg cggcgcagcg ggcggtttgt
    31021 ttatcggcct gatagcctgg tggggtctgc cgatgggctt aaacagcgcc tttgggccgt
    31081 ctggtctggt ggcgctgccg ctgatgactt ccgcacaagg catccttccg gcaatggcgg
    31141 tttatgctgg cgggattctg gtggcatggg tttgcgggtt tattttcacc acgctctttg
    31201 gctgccgtaa cgtcaatctg gactgatatc atgaaacgga caatgctcta tctttctctg
    31261 ctggctgtta gttgtagcgt cagcgccgca aaataccctg ttctgacaga aagttcgcca
    31321 gagaaagcag ggttcaacgt cgaacggctt aaccagatgg atcgctggat tagccagcaa
    31381 gtggacgccg gttatcccgg cgtaaacctg ctgatcatta aagataatca gattgtgtat
    31441 cgcaaagcct ggggagcggc gaaaaagtac gatggcagcg tgttgatgga acagcctgtc
    31501 aaagccacca ccgggacgct gtatgacctg gcctcaaaca ccaaaatgta cgccacgaac
    31561 ttcgccctgc aaaagctgat gagcgaaggc aaactgcatc ctgacgatcg gattgcgaaa
    31621 tatattccgg ggtttgccga tagtccaaat gacaccatca aaggcaaaaa cacgctgcgg
    31681 atttctgacc tgctgcatca cagcggcggt ttcccggcgg acccacaata tccgaataaa
    31741 gcggtcgcgg gcgcgttata ttcccaggat aaaggccaga cgctggagat gatcaagcgc
    31801 acgccgctgg agtatcagcc cggcagcaag catatttaca gtgatgtcga ttatatgctg
    31861 cttggattta tcgtcgagtc ggtcaccggt cagccgcttg accgctatgt tgaagagtcg
    31921 atttatcgcc cgctcggcct gacacatacg gtatttaacc cgctgctgaa aggctttaaa
    31981 ccgcaacaaa ttgccgccac ggaattgaac ggcaataccc gcgatggcgt gatccatttc
    32041 ccgaatatcc gcacctccac tctctggggt caggtgcacg atgaaaaagc cttttattcg
    32101 atgggcggcg tttccgggca cgcaggtttg ttttccaata ccggcgatat tgcggtgtta
    32161 atgcaaacga tgctgaacgg cgggggctat ggcgatgtgc agctgttcag tgcggaaacg
    32221 gtgaagatgt tcaccaccag ctctaaggaa gatgccactt ttggcctcgg ctggcgcgtg
    32281 aatggtaatg ccaccatgac gccgacgttt ggcacgctgg caagcccgca aacctacggt
    32341 cacactggct ggaccggaac ggtgaccgtt atcgatccgg tgaatcatat ggcgattgtg
    32401 atgttaagca acaagccaca ttcgccggtt gccgatccgc aaaagaatcc caatatgttc
    32461 gaaagcggtc agctgccgat tgcaacgtat ggttgggtag ttgatcaggt gtatgcggcg
    32521 ttaaagcaaa agtaataaaa aggccagtca gtaatgttac tgcctggcct ttaatcaatg
    32581 aatcagaaac gcttacagcg ccatcaactt gtccagcgac ggtgcgaaat aatagccgcc
    32641 ggttaccggt ttggtgaaac gcaacatcgc atcacgctta ccatcggtat cgccaaacat
    32701 gctcagcagt tgctgctcaa tgttatgcag acgcgcgcag taggcgcaga agtacagacc
    32761 gtgagtgcca ctggcagtgc cgtacggcag gctctggcga acaatcttca gccctttgcc
    32821 atcttctttc agatcaacgc gggtgaggtg agaggtttcc ggacgttcgt cgccgtcgat
    32881 ctcttcgttg gcctctttgg tgcgcccgat catcatctcc tgatcgtgaa cgctcatccg
    32941 gttgagctgc ttcaggttgt gttcccaacg ctggacaaac acatagctgc cgcccgcatc
    33001 cacgccgtct ttgataaccg ccacttcgcg acgcgtctct tcacccgccg ggttttccgt
    33061 accgtcaaca aagccgctca ggtcacgctc ttcaacccaa cggaagccgt ggatctcttc
    33121 tttcacttca atgcagtcac caaaggcttc catcgccgcc tgggcgacag agaagtttac
    33181 gtcgtgacgc agagaaagaa tgtggatcaa cacatcgaac tgggtggtcg gcgcaaggcc
    33241 tttaccgtag cccggaaaat ctttcagctc ttctgcccca acgccgccgc tcagagcgcg
    33301 ccaggtgttg ttaccaaagg caaccaccgc accaagatgc gcgtccggga atttcgcttc
    33361 aaaagttgcc agtttgtcgg caaatgtttt actggccgca cgcagggcgt caacttcccc
    33421 tttcacgttg gcttcgatcc aaatcgccgc gcggcaatgt tctggcaaaa tgccactctg
    33481 aacctgagac attgttcctc ctgaaaataa taatgccacg caatccgtgg cattgttgag
    33541 cgctatttta cgtgtttttt cgcggcggcg gtttgttcag acgcaaatta acgccgccag
    33601 ataattttac tgactttcca gtttttcagg gtatcgtccg aaggcattaa cccttcagga
    33661 ccactccatt ccccgctgaa ctggtagcta atatgttgac tgccttcggc tttacattcg
    33721 acggcacgat tatcatcacc atcagctttt tggcaattgc caaatgcttt gctgtaaagg
    33781 tcgctaaacg gtgtaccgat tttaacaccg gtgtcagcag gaatatcgct atccagcacg
    33841 tcaatgcgac tgatcgtgcc ttgatcgccg ttaatcacca tcgccacgtt gtcgcccttc
    33901 atcacttcaa aaaagcgcac cacgttgccg ttcgcggttt tcattccgct gcgcaggcga
    33961 taatcgccat caagcgcatc ggcaatggct tgttcttgca gtggtgtgga cgccgttaat
    34021 tcaccaacgc cctgctcgct cactttggtg gacgacccaa accagttcca cgggttagcg
    34081 gccgaccagt taactgacga catcgtggaa cagccggtca gcatcaacgg catagcgcat
    34141 aacattaaac gcagcgattt catgtcactt cctttggtta ttcaataacg ttgcttggag
    34201 tgcaaattca ccaaaaagtg ccgttattct ttttcttcat taaaacaagc gcgtaaacgt
    34261 cgattggtca gcagccagat taacgccaca atatccgcca ctaccagcgc cagaccgata
    34321 ccgctaacgg attcaccgtt cagccacagc cacggttgcc agcaaagtaa aaccacctgc
    34381 gccaacagca acagaaaata gagcacatgc caagtgcgag gaaatgtagc ccgccgaccg
    34441 ctcaacaaaa acgccagcac cgccggaatg cctggaatca gccccagcca gaaattatcg
    34501 tgatcgggat aaaacagatt tagcagcgca gtaccctgct cgcgcgacgc accggcaatg
    34561 acaaacagca cccaggttcg cgcctgaagc aatagcacaa gccagaagag caagggtaaa
    34621 cgcaggcgtc cgtgcgcatc ataatggaca ggatggaact cagtactctt catcttcaat
    34681 caaacgctta cccagactca gcacgtcggc gtgttcatat cccaggcgtt catacattcc
    34741 gagcaccatg tcgttatctt ccggcacatt gatctgaatt ttcgggcagc cacgagcaat
    34801 cagctttttc tccagccgat taagcaacgc attggcaatc ccacgcccac gaaactctgg
    34861 atgcacgcca agataataag cagacccgcg atgcccgtca taaccgccca tcaccgttcc
    34921 gaccacttca ccgtttacct cagcgaccaa aaacaaactg acgtcatggt tcatcttacg
    34981 ctcgatgtcc atttccggat cgttccacgg acgcagcaag tcgcaacgct cccaaagggt
    35041 gatgacctct tcgaaatctt cctggcgaaa tacgcgtatc tccatggtat tcgttacctt
    35101 tttgcgggtt aaaaggctga ttatggcgtg aacggtcgaa ttagccaata tctgacgaaa
    35161 atcggttgaa aaagtggcat aatggggagt tgtcaactat tgaaatgaaa agtaaaacaa
    35221 ttctcaacat caaaccgtcg taacggatta cgcgatacga tataacatct ggaactttat
    35281 tattacaact caggccgtat gagcactttt aaaccactaa aaacactcac ttcgcgccgc
    35341 caggtgctga aagccggttt ggctgccctg acgttgtcag gaatgtcgca agccatcgcc
    35401 aaagacgaac ctttaaaaac cagcaacgga cacagcaagc cgaaagccaa aaaatctggc
    35461 ggcaaacgtg tcgttgttct cgatccaggc cacggcggaa ttgataccgg agcgatcgga
    35521 cgcaacggtt cgaaagaaaa acatgtggtg ctggcgattg ctaaaaacgt ccgttccatt
    35581 ttgcgtaatc atgggattga tgcgcgttta acgcgttctg gcgatacgtt tatcccactt
    35641 tacgatcgcg ttgaaatcgc ccataaacat ggcgcagatc tgtttatgtc aattcatgcc
    35701 gatggcttta ccaacccgaa agctgccggt gcttcggtat ttgccctctc taaccgtggg
    35761 gcaagtagcg caatggcgaa atacctgtct gaacgcgaaa accgcgccga tgaagttgcc
    35821 ggtaaaaagg cgactgacaa ggatcaccta ttgcagcaag tgctgtttga tctggtgcaa
    35881 accgatacta ttaaaaacag cctgacgctc ggctcgcata ttctgaaaaa gattaagccg
    35941 gtgcataaac tgcacagccg caacaccgaa caagccgcgt ttgtggtgtt gaaatcgccg
    36001 tcggttcctt cggtgctggt ggaaacctcg tttatcacca acccggaaga agagcggctg
    36061 ttaggcacgg cggcgtttcg tcagaaaatc gccacagcga ttgctgaagg cgtgatcagt
    36121 tatttccact ggttcgacaa ccagaaagca cattcgaaaa agcgataagt tatgaaaccc
    36181 gacgcacacc aggttaaaca gtttctgctc aaccttcagg atacgatttg tcagcagctg
    36241 accgccgtcg atggcgcaga atttgtcgaa gatagttggc agcgcgaagc tggcggcggc
    36301 gggcgtagtc gggtgttgcg taatggtggt gttttcgaac aggcaggcgt caacttttct
    36361 cacgtccacg gcgaggcgat gccagcttcc gccaccgctc atcgcccgga acttgccggg
    36421 cgcagtttcg aggcgatggg cgtttcactg gtagtgcatc cgcataaccc gtatgttccc
    36481 accagccacg cgaatgtgcg tttttttatt gccgaaaaac cgggtgcaga gcccgtctgg
    36541 tggtttggcg gcggcttcga tttaacccct ttctatggtt ttgaagaaga cgccattcac
    36601 tggcaccgca ccgcccgtga cctgtgcctg ccatttggtg aagacgttta tccccgttac
    36661 aaaaagtggt gcgacgatta cttctacctc aaacatcgca acgaacagcg cggtattggc
    36721 gggctgttct ttgatgatct gaacacgcca gatttcgacc actgttttgc ctttatgcag
    36781 gcggtaggca aaggctacac cgacgcttat ttaccaattg tagagcgacg taaagcgatg
    36841 gcctacggcg agcgcgagcg caattttcag ctctaccgtc gcggtcgtta tgtcgagttc
    36901 aatctggtct gggatcgcgg cactctgttt ggcctgcaaa ctggcgggcg caccgagtcg
    36961 atcctgatgt caatgccgcc actggtacgc tgggagtatg attatcagcc aaaagatggc
    37021 agcccagaag cggcgttaag tgagtttatt aaggtcaggg attgggtgta actccctcac
    37081 ccccattccc gcatccgctg atgcaacgtc aacgacggct tctcggcaaa cagctgctgg
    37141 taatccgtgg caaattgccc cagatgccag aatccccact gcatggcggc gtcttttacc
    37201 gttgtgcttt gcgaccacgg gcttatcagt tcgcggcgta cggcgttcag gcgaatgcgt
    37261 ttcagccacg cgtttgggcc aatgcctaaa atagcgtgaa acgcgttttg tagcgtgcgg
    37321 cgactgacat gcagttgatt acacaagtcc agcaccgtca ccggctcaga catattttcc
    37381 agcacatatt cacgggcgcg ggaaagtaat ctacggtaac tctgatgact gatgctttcc
    37441 gccgtcacca ttggttgcgc gtcttccagc atcgccccca tcgccattag caaattatcc
    37501 cccagcactt ttcgcactgc aggctgatgg agattttccg ggttctcgca aaacgtcgcc
    37561 agcgcctgtt ggacaaagcc ccacagcgcg gctttatgct gctctttcac ttccagcgcc
    37621 gactggctac gcagcatatg taatacccga tccgggttat gcaaaaagtt agcctgccgg
    37681 gtgatgacat cttcagaaag caccacgccc aggatcgtgt aatcatccgg cgtgctcagt
    37741 tcaaattcag tgccaccagg gcgggtggcg atttccgcgc ttcccagaca ttgcgaaccg
    37801 ataaatccct gctcaccgcg cgtcgccgga atgccaaacc agaacgagtt cggccagacc
    37861 aggcacgact gacgcagcgc cagaccggtg tattcacgaa aaacctgaat atcatcgagt
    37921 agaatttccg taaattcacc atgaaacttg cccggatgca gctgatcgta aatctgctgc
    37981 caggcggtaa tcgttaaagc atgttcatag acatccgttg tctgtcgttg atgaacatta
    38041 tccacttcga ccttcggcgt gagcttcagg ttttcgggta agggttcatg ataaagatgg
    38101 tgcaaattgg ctgtacgggt ctttttcatg atgttaatgc cgggtgttgt aggacacccg
    38161 gcacctccga caggttaatg gggcttgaga cgataacgac tactgcgttt acataacgtc
    38221 ccggcagaaa agagctgttc gagcgcattt ctggcttttt ccagcggcca gccaaagtgg
    38281 gcagccactt ctcctgccgt cattccctga cgtactgatg ttaacagcgc caacaactcg
    38341 tcagcagatt ccgcgacgat aactggcgcg tcgggttccc ttacgggttg cttcggctgg
    38401 cggttaaagc cagtaaccag ccactgggtg tcatcgtctg gccgaccgat ctccttgcgg
    38461 ctgatgacac gcccggtacg catcgcggca gcacaacctg cgtccagcgc cgcacgacac
    38521 gccgccagat cgccttccac caccagcgtt aagcgaccag ggtcaagcac ttcgtgactg
    38581 agcagacgaa cgttagctgc tttgagcatg gcatccgctg catctatcgc ggcgaccatg
    38641 ccgtccactt ccagcaatcc cagtgcattg atcatcggca acctccgtta cgcacgctgg
    38701 attggattac gcgcgatttc cagcactgca tcggtaaagg cgttacaggc tgctttacac
    38761 gcggcctggc taccggttaa aaatgctgcc gagtagttgg tttcagacgg tggcgggaca
    38821 taggtcacca gctgtacgtc ggcagatttc aacgctgcat caatgccata ggtcgcttcc
    38881 agcggcggtg ccaccagata cgccatcgga tcgccaagcg tgatcccggc ggttgatgag
    38941 agataagaac cggtacgcga aaccacatgc gccaggaagg cggtgttttc tgcgtcattg
    39001 gcccactgga atgctgcacc attttcaata tgtgcaacca tcgcatccag accggcacgc
    39061 acttccgccg ggtttggccc accaagcata atcagcactt caccggcagt cggtgacggg
    39121 ccgtgtgcag cgcccgcata cagcgagcga ccatatacca cttccaccat cgcctgcttg
    39181 gtcgcttcat cagcggcaat gtaggtgacg tcatcggaat cagcagaaat cagcccgagg
    39241 ctacgaatat gcggcggcaa ttttagttca cgcgcaaatt cagcgttaac ggaggcaatc
    39301 acccgcatgg cggttaccga cggtcgaatc aaatctaaag ctggcatgat gcctccttat
    39361 cgggtcatgt tgatgccgga tgctttctgc tccagcatac gtttcgccaa atccacaatg
    39421 acggctgcgg cttcaaccgg cggcgtgccg ccctggtgaa tgttagagat acaggtgcga
    39481 tcggcctcaa cggtggtcgc catacgcggc gagtaaacgg cgtagcagga gaggctttct
    39541 gactgcccta accccggacg ttcccccacc agcaggatca ccactttcgc gccgaggatc
    39601 tcaccgatct gatcttcaat cttcacgcga ccataacgaa caaagaatgg cgtgccgact
    39661 ttcagcccgg cctgtttcag gcccgccatc agcggcggca ggatctcttc gtagttcacg
    39721 gtgatggcat cagttgacag gccatcagaa ataacgacct gtacgtccgg attagcaaca
    39781 cactgtgctt tcagcgcttc aacagcttct gcacacaggc ggcggcccat atccgggcga
    39841 gtcaggtaga gatttttgtc gctaatttca gagcgtactt ccagcaagcc ctgcgctttc
    39901 acccactctt ccggcacttc tttcagtacg gtatctttcg aacgagagtg atcggccagg
    39961 aaacgcagca gcgcctgggt acgtggacgc ggaccggcac gaccggtaca aacgcgggcc
    40021 acggtgctgc ggcgcagttc tgttaatacg tctgcgcgat gcgggttttc aacaccaatc
    40081 cacgcttttg cttcagcgga acctaaatcc agcgcacagc tttccgaggt caccggtgcc
    40141 gcacagttgg tggtggcgca ctttgcttct gacggcgcgg gggccgtttg tcccattgac
    40201 gccatcacgc tgcgtacaat ttcttcaatc tgtttttgat ccatgatgtg ttatctccgc
    40261 gtcatcagaa gaacagtgac ggatcgcccg cccgtttggt caggcgaccg tttgccataa
    40321 tgcccatgct ttccagccag cgttcaaact ctggtgacgg gcgcaggttg agtaactgac
    40381 gcacagtggc ggtatcgtgg aatgcggtgg tctggtagtt gagcatgatg tcatcaccca
    40441 gcggcatccc catgatgtag ttgcagcctg cggtggcgag caggatcatc aggttttcgt
    40501 tgaggttctg gtcagcgtca gcgtggttgg tgtagcagca gtcacagccc atagagatgc
    40561 cgctcagctt gcccataaag tgatcttcta agccagcacg gataatctgg cggtcgttgt
    40621 agagatactc cggcccaata aagccgacca cggtgttgac gataaacgga tcgtaatgac
    40681 gcgccagccc atagttacgt gcttccatcg tcacctggtc agcgccgaag ttagcgccag
    40741 cggatagcgc agagccttgt ccggtttcga agtagaggca gttttccccg gcgatacggt
    40801 tgaactctgc acccactgcg cgcgcttcgt cgagcatcgc cagttccacg ccaaactctt
    40861 tcagcccttt ttcgctgcca cagatactct ggaaaatcag tccgccaggc gcgccgcgac
    40921 gaatcgcttc gatctgggtg gtgacgtgcg ccagtacaca gccctgagtt gggatgttga
    40981 atttgtcgat cacgccgtaa atggtgtcca acacgcggct taagttttcc acgtcgtcag
    41041 tcaccgggtt aacgccgatc accgcatcgc ccaccccgaa ggaaagccct tcgtagattt
    41101 gcgcggcgat actttgcacg tcgtcacggg tgtcatttgg ctgcaaacgg gcgctgaagg
    41161 tgcccggaat accaatggtg gtattggcct ttttgattac cggcattttc ttcgcgccgt
    41221 agatcaggtc cgcgttggag caaatcttcg ctaccgccgc gaccacttcc gaggtcagcc
    41281 ctttgcgggt aaaggcaatg tcgtccacgc tggtttcatc gctcagcaca tactcacgca
    41341 gttcgctgat gctccagttt ttaatctggt tgtaggccgt ttcgttaaca tcgtcctgaa
    41401 tcagccgcgt cacgcagtca tcttcatagg caatcaccgg attattgcgg atgtccgcta
    41461 cggtcatttc cgacaacacc tgctttgccg ccacgcgctc ctgtgagctt gccgctgcaa
    41521 cgcccgccag cacatccccc gaacgcagtt cgttggcttt agccagcacc tcttttacat
    41581 ccttaaactg atatacattg ccgaacaatg tggtctttag tttcataagt cgttccctca
    41641 ggaaggaaat gcgagtgatt tcaccgtcac cggcacaacc gatccgccaa aaagaggcgt
    41701 accaatgtcg atatagtccc ccgcgcggac aatcacttcg tcaatgactg ccaacgggag
    41761 ttgttgtagc tgtgggcgca acaacatgcc cagagcttta ccaaagtcct gcccggccac
    41821 caccagcagg ggatgcggat tcggaaaacg cgcgacgaaa tcgaccagcg cgttgatgac
    41881 cgtcagtacc gcggcgtaac gcacaggcag cgaggcggga agcgccagca cgtacgcgtc
    41941 agttttggga tcaagatcca gctgaagcag cgcctgttgc caggcactca ccagatccgt
    42001 ttcatcaatc gggatcgcca ccggcaaatt gcgcagcggc agttgtacgc cctccagcca
    42061 gattgtgctg ccagagagcg aaagggtatg tgcacccgcg ccaatcaccg tggcgcgtac
    42121 ggtttgcgcc ggaaactgca cattcatctc acgcaggcgc ggatggtcat gcagcgccgt
    42181 tgccagaagt gggccaatat cggcaaaaca gaacgggtcg gcgggctggt ggcgataaca
    42241 ttcgcccacg cctccagaaa gcgtaatgat ttcgggcgta acacctgcgg gcagcaaacc
    42301 ggtttgcatc aatgcctgcg cgagcggcga aagcgttccg tcaatcactt cgacaatcag
    42361 cgccgccatc cgccgggtaa cctgcaccag ctgcgcgccg gtcagcgaac gggcgtcagt
    42421 gcctgcaccg aagcactcat ccacaatcat ctgccccggt ttatgagcgt aaaccacgcg
    42481 cccctggctg tcggtttcca gcaggcgacc accgacgttg agacaggcag tgccgctgat
    42541 ttttccggca tcgaacaggg cgtagttcgc ggtgccaccg ccgatgtcga tattcagtac
    42601 ccgacacagc cgttgttcag aaagggtttg cgccccagct ccgtgaccgg cgatcacgga
    42661 ttcgaggtgc ggcccggcgc tggcaacgac aaaatccccc agcgattgag agagcgccat
    42721 caccgccggg cgagcattgc gggttttcgc gctttcaccg gtgatgatga tggcaccaga
    42781 atcaacgctt tccggcgcaa tacccgcagc ctgatattgc tcgagtatta aggttttcag
    42841 ttccgcttct tttaaaccgc cctgtttatc gacaggggta aagaacaccg gactttgcca
    42901 gctaatttcg cgtttaatga attcgtagcg cggcacctgc gacaccgccg cacggttaac
    42961 cagctccagg tgggagaaaa tcacctgggt ggtggtggtg ccgatatcga taccgacgct
    43021 caatagctgg cgagtgttca cgattgtgcc tccgcttcgg ttttagtcgc ggtcgcgtct
    43081 tcttttggca ccagcatcat cgccacgcca atcgccgtta cgccgccgat caacttgccg
    43141 acaatcatcg ggaagatcat ggcgttcatg ttggcagcgg cgaagcctaa gtggtcgccc
    43201 agggcgaaag cagcggaaac ggcgaaggcg cagttgatga ctttgccgcg ggtatccatc
    43261 tgcttcatca tgccgaacat cgggatgttg ttggcaagcg ttgccaccat gccggctgcc
    43321 gcgatgttgt tcatattcag cactttaccg acgctcatca gcggtttttc aaaccagcga
    43381 gtcagcagca gcaccattgg atacgcccct aacagcacgc aggagataga accgataact
    43441 tcaatggcgc gcatcacttc accgggttta tcgccagggg ccataaagat aggatccagt
    43501 ccggggatca gttcccagcc aaggaggaat ttcactaccg cagcggcaag accgagggtg
    43561 atcaatgcaa cgaggaattt ggcgaagatc tggaagccgt tgatcatttt ttccgggatg
    43621 aatttcagcc ccagcgccac cagaatcgcg acaatgatca ccgggatcat gttcatcagg
    43681 atcagggcga aagtgaattc caccggctga ccgttgatct gcacaccgga gtacatagca
    43741 accagaccac cagcaataca gccaatcgga atggtcacaa tgcccgccag cacgccgagc
    43801 gccagataac gacggtcaga aggttcgata atgccgagcg ccaccggaat ggaaaacaca
    43861 atcgttggcc ccatcatcga cccgagaatt aacccagagt atagccacgc tgctacgtcg
    43921 ccgcccgcca gctctttggc gaggaagaag ccgcccatat cgcacgccag cagtgttccg
    43981 gcgaacatcg atgggttagc gccgagcatt tcgtaaaccg gaataattac cggcccgagt
    44041 acgtgagcca gtaccggtgc cagcgcggtc ataccgacca tcgccaggcc cagtgcgccc
    44101 attgccataa agccttcttc gaactgaccg cctgatcctt cgatactttt accgaactta
    44161 ccgaggaaac gagcagaacc gccgaactgc gacaggatcc tgtctacggc agctatcagc
    44221 ataaagaaca tcatgatgta catgatgatt tcgttaattc ccatcgcctt tactccctgt
    44281 tagttgttat ttattggcgg atgcggcgta aacgccttat ccgccctaca tgtgcaatcc
    44341 cgaaggccgg atcagacacg gcaagcgtcg catccggcat ttattgcgcc gctgcgtaca
    44401 ggccgacaat ctgctctagg ctggcggtac gcgggttgct gcgcagacaa atatcttcct
    44461 gcgcggcctg cgcccatgca ccgtaatgcg cagatgtcgc gccaacatcg cccagtcgtt
    44521 taccaatccc aacttccgca atcagctcac ttaccgcgtt aatagcgtca cgatcgtcgg
    44581 attttttagt tcgcagtgcc cgaccaatct gactaaaccg ttcgcgacaa accatccggt
    44641 taaattccat caccgttggc agcaacatgg cgttcgccag accgtgtgga atatgcagcg
    44701 ccgcccctgg ctgatgcgcc atcgcgtggc acaacccaag ccccgcactg gaaaacgcca
    44761 ttcccgccat acatgaagcc agtaacatgc tctcgcgcgc ggcaaggtca tgaccgtagc
    44821 ccaccgcttt cggcagcgat ttgccaatca tcgcaatggc accaatcgcc agactgtcgg
    44881 taaacggtgt ggcgttcagc gcgctgtacg cttcaatggc atgggttaac gcatcaatgc
    44941 cagtcatcgc cgtgacatgc gacggcacac cttcggtcag cgcggcgtcg aggatcgcca
    45001 catccggcat cagcgaggca tgggctaaca cctgcttgcg cccgctcacc gtgtcgataa
    45061 tcaccgttac gttggtggtt tcagagccgg ttccggcggt agttggaatg gcaatcaacg
    45121 gcaggcgcgg ttgcagaacg ctggtttctg acatttccgc cagcgtgcta tccgggttcg
    45181 tcaccagcaa cgccacggct ttcgccgcat ccagcaccga gccgccgcca aatgcgatca
    45241 ccccatcaca gcctgactca cgcaactgcg ccacggctgc acacacgtcg gtgatgcacg
    45301 gttcgcccac cggacatggc cagagcgtca tggcgatgcc tttaacagcc aggctgcgcg
    45361 tcagcccggc ggtcatccct gcctgatgca aaaagctgtc tgccatcacg aacagatgtt
    45421 tcagcccacg cgtttgcgct tgctgtccgc aactgctcac cgagcccgga ccgcaaagcg
    45481 tcaccggtgg aacgctaaat gtttttaccc gttgcagatt cagggtatcg aacgcctgaa
    45541 agagcgcggt ctgcaattca ttttgcatat agcccctccg ctttctctcg cccactactg
    45601 gcgatcgcca gcggggtcat aaacaaactg tgctgcggta aatgcacttg taacgccggg
    45661 aattgtttgc gaaacagttc cgccacgccc ggttgcatac aggagccgcc cgccagccat
    45721 aaatcagtaa tgccctgccc ttcaatatgc cgggcgacaa tgtccgccat tttttcgtac
    45781 accggtttca ccgcaggcca aatctcttca ccgtgaccgc gcttgtactg ctccgcctct
    45841 tccagcgaaa tacggcgatt tccggcgaga gtcagagaga tgtgatgtcc gccggtggct
    45901 tcatccgccg agtacgtcac tttgcccttt ttcacgatgg caatgccggt agtgccgccg
    45961 ccgatatcca ctacaccggc gttgtccagt tgcagcagat ccgcaactgc ggttggctca
    46021 tccagcacat ggctcacttc cagtccagca gactccagca cgttgatgga aatacgcggg
    46081 tccgtacctg gaggaaatga agtcgccgca tggctaaaac ggcgaccgaa ttgctgctcg
    46141 agcgtgtcga gatggcgacg aacaatggtg acagcgccga agaaatccca gacgatgccg
    46201 tcgcggacca catcggccca gtcgaggcat accgccaccg gctgaccgtc gcggtcgaca
    46261 accatcgaca ccacatcgca ggtgcccaga tccaccccca gccacagcgg agattccgtc
    46321 gcggcgggcg tctggttaca cagcgttgcc gccgtttgca gacgtggggt gagccattgt
    46381 tcgtcgtgcg ccatcggtta ctccttaaac aatgcgaaac gcatcgacta atacacagcg
    46441 acgcagacgg acaaacgtcc gcgcgctggt taccccttca ccggttggcg tggtgatggt
    46501 catggtggtc cagccttccc cgcccagccc cagcccggca atgcacggtc cgttcttaac
    46561 gaaaatgctg gtatcaatag cattcgccat ctggttcatg ttttcgatgt tgcgcgagtg
    46621 cattgccgcc gtgtggtggc aaccgccttc cagtttcacc gccagcgcaa tggcatccgc
    46681 cacgttggcg acgcgcacga cgggcagcac cggcatcatc agttcggtca cggcaaacgg
    46741 atgttctgca gtggtttcca caaacagcaa acgcgtttct tgcggcacgt tcaggccgat
    46801 tgccgccgcg attttcgccg catcgcgacc aacccagtca cggctgacgg tgccttttcc
    46861 gcgctcgtcg atatttttca gcaacaccgg ttgcagctgc tgcgcctgtt ctgcggtcag
    46921 tttcaccgcg tgctggcctt ccatcagacg catcagttca tcggctacgc tatcaacaac
    46981 aatcagtacc ttttcgtcgg cacaaatgat gttgttatcg aaagaagcgc ctttgacgat
    47041 ggactgagcg gcacgggcga ggtcggcggt ttcatccacc actaccggcg ggttgccagc
    47101 gcctgcggca atcagacgtt tattggtgtg tttacgcgcc gcttctacta ccgcttcgcc
    47161 gccggtcacc accagaaggc cgatgcccgg aaacttgaac aaacgctgcg cggtttcgat
    47221 atccggattt gccacagtaa ccagtaagtt ttccggcccg cctgcggcaa caatcgcctg
    47281 gttgagcagc gtgatcgccc gctgggagac ttttttcgcc gccggatgcg gggcaaaaat
    47341 gacgctgttg cccgcggcaa tcaggctgat ggcgttatta attacggttg ccgccgggtt
    47401 agtggacgga gttaccgaag ccaccacgcc ccagggcgcg ttttcaatta aagtcaggcc
    47461 gttgtcgccg gtcagcacct gcggagagag gcactcaacg cctggtgtgc cgcgcgcctg
    47521 agcgacgttt ttggcaaatt tatcttcaac gcgccccatg ccggtttcac tgacggcaag
    47581 ttccgctaaa tctctggcgt gtttttcgcc tgcttcacga atggcagcaa tggctaactg
    47641 gcgcattgcc acgcttttta acccttgctg ggcgactttg gctgccgcaa cggcgtcatc
    47701 cagggacgcg aaaacgccca tctcatgaac ggcggcggac ggcgtgtcac tgctttgcat
    47761 tttcagcagt accgctttca ccacctgttc aatatcctgt tgattcatga tgttctgcct
    47821 tatttgtgga aaattacctg accgccagac accacctcat cgacaatgcc aatcacgcac
    47881 agatcgaccg gtgacgtttc gcttttatgc gcctggcggg cggaactgcc actcaccagt
    47941 aacacccact ccccggttcc cgcgccaata ttgtcgatgg cgacggcgca ttgcccgtca
    48001 ggattacctt gtggatcaat catttccacc atcagcaatt tgtcatgcgc cagtccgtga
    48061 tggcgtacgg tacaaacaat ttgtccagtg acgactgcca gtttcatacc cgcctccgtg
    48121 gcgtatttca ggtaaaagct ccccctaccc tccgcagaag gtaaaatgaa aaaggagaga
    48181 gcgtgacgcc cgaatcgacg tcacacaggg tgattacagg ttgctgctat cgcctttcag
    48241 gccgatcggg aagacttctt ccaggtcgcc gtgcggacgc gggataacgt gtacagatac
    48301 cagctcgccg atacgctgtg ccgctgcggc acctgcatcg gtcgcggctt tacaggctgc
    48361 aacatcacca cgaaccatgg cagtacacag gccgccgcca atctgcttaa caccaaccag
    48421 cttgacgcgc gcagctttca ccatcgcgtc agaagcctca atcagtgcaa ccaggccccg
    48481 ggtttcgatc attcctaatg cttccattgt gctttcctct ttatcagggt ccagaacggg
    48541 accgttcatt caaccagtgt ttgtaaactg ctttcgcggt tcacttctgt ctgacgcggc
    48601 acagctgcca ccagcgccag ctcgataatt tcctgcacgc tacaaccacg agagagatcg
    48661 tgcatcggcg cggcaagtcc ttgtatcagt ggcccgacgg cacgatatcc gccgagtcgt
    48721 tgtgcgattt tgtaaccaat atttccggct tccagcgacg gaaaaaccat cacattggcc
    48781 ttgccctgta gcgggctggc aggcgctttt tgcgccgcca cttccggcac gaaggcggcg
    48841 tcaaactgta actcgccatc caccaccagc tttggtgcgc gctcacggac gatttctgtc
    48901 gcctgctgga cgttagcaac acaggggtga cgggcgctac cattgctgga aaacgacagc
    48961 atcgccacgc gcggctcttc tccggtgatg gcgcgccagg tttcggcact ggcaagcgcg
    49021 atatccgcca gctgcgccgc cgtcggctgt ggcaccacgc tgcaatcggc aaagcccaac
    49081 gccgggccgc tgtactgtgg caacatcagg aaaatggaag agagcgtttt acagcctggc
    49141 tgcaagccga taatgcgtaa tccggcacgc agcacatttg ccgtggaaga gaggttgccc
    49201 gcgatacaga catccgcttt accggcgctg accattgctg cggcgaacat cagagggtcg
    49261 gtaagttttt ccagcgcatc cggcggcgtt ttttcgcccg cgcgggccag ccagcgatga
    49321 gcaaattctt cccgcattgc gaggttgcca tgcggatcaa tcacctgtag cccgtccatc
    49381 gccacgccgt gactgagcgc aaactgacga agttcaaacg gattggcgac cagaatgggc
    49441 gttgccagac cttgttgatg taaatattgc gcggctttca gcacacgttg gtctaacgca
    49501 tccggaaaaa ccactctggc gggcgctcgc aacgccagtt cacgacaacg ttcaataatc
    49561 attgcgtttc ccccagtcgt tgttggatct gcttcaccgt tagcggctgc ttcgttacgc
    49621 tcaggatcat catcacgtag accgtgctcg aaaggcggtt cagcgcctgc aaaatgtccg
    49681 ggcgtaatac ttcaaagctg cgggtgataa acacctgcgc cgctacggtt tcggtttccc
    49741 gcactttggt acgtagcaga ttgagcaacg ccgcatcgcg cccgtggctg gcttccggca
    49801 ccagatgatc gtggtcgaga tagcgcagcg gctgatgaga aagccgatgc agatcttcgt
    49861 cagaaagccc gacaatcgcc tggcagccca gcggttcccc cagcgcatcg gcgcgcataa
    49921 tgttgccaag acgcgaacgg atatccgcta gccacggctg ccacggttcc gccagttcaa
    49981 tttgcagcca cactgccagc gcaatggtgc tgtcgagaac cgcgcgaaaa cccagacgcg
    50041 gatcgctttt ggcgaccatt ttttccgccg acaggtgagt cagcgtatcc ggcttttttg
    50101 ccaccggctg gcggcacagt tcgcagcacg cctgcggatg ttcatcgcta ctggtcagcc
    50161 catgaacggg ctgcggctgc tgctgttcat cgtcaacaaa caggcggccc tgctcgtcaa
    50221 taaacttgat gcgcagatgg cggctttcca gcaactcccg ggcagagggc gtcaggcgac
    50281 tgtccgcagg gagatggatc tctgcgcctt cgctgagcgt atggttcgct cttagccatg
    50341 cttcggtgat gaaatctttc atagggattg ccagttagcc ggccaggcga catacaggaa
    50401 tttcacgctg gatgtcgtac caaattcgat gctggagcct ttcgggataa acatcacgtc
    50461 gcctgctttg gcgatcatgg tttcgccttc gtggcgaaca tgtagctccc cttccagcac
    50521 catgtcgatt tcgtcgtagt tcagggtcca cgggaagaat gcgttctccc actgcataaa
    50581 accggcggcc atgctgctgc cgtcgtctcc ggtcacaaga tcagtcaagc ccacgcaatg
    50641 cggctctgcg ccgtcgaaac gaccaaactt gacgctgctg ccatcaatca ctttgatgcc
    50701 gcctttgccg gtcaccgatt tgaagctcgg ctgcattgcg ccctgctcca gcgactgttt
    50761 ttccttcatc actttttcca tcagctgtgc gactaggctt tcggtaaact ggccttccgg
    50821 cagctgggca atgatggttt cgcggatgcg ctggctttcg gttttgtcgg ctggcacgct
    50881 ggcgggcacg gacgccgtta ccgggatcga ttcatcacat tcggtgatgg taaaacccag
    50941 tagatccgcc acttcacggg cttccggggt aataatgctg gcgcgcagaa ccacggacat
    51001 tgcctgttcg ccgcgcgcgt gtgcttcacg aatatcattc gctgtgatga gttttttcac
    51061 ctgcttcctc ctgtttggtg agagatgcca gataatccac cagttgctgc acgctttgcg
    51121 ggtcgtggct gtttagctcg aaaatcggct cttcgaagcc agtctcaagc aataactttc
    51181 gcgttgcggc gacgtcagca tctggcatgt ccgttttact gatgacggcg atttgccgtt
    51241 tgctgacgcc aatatccagc aacccggcag gcaaccgact ttccgggtca tttgctccat
    51301 ggacatagat cagcatgtct acatcctgta gcgtggtaat taaggcgtga taccagcggg
    51361 gatggctaaa atattcaccc ggcgtatcaa tatcgccctt atcattaaat tccacggcct
    51421 gtgtttttct ggcgagggta taattcccct gcaacgcatt aaataacgtt gtttttcctg
    51481 ccccgacgct acccacaaac gcaattcgtt tcatgaatgc ctcgtattaa cttttggtca
    51541 tttcacaaag cgtataattt aataagcgac ctaaaccgct gacggtttgt gataacgctt
    51601 cttctactgc gcccaccgag ccataaatca ccaatgcacc gctgaagcgg tcgagaaaac
    51661 cgatatgcac atcagcggct ttcagcgcta aatcaccggc gatcatcgct gtttcgccgg
    51721 gagttagcgt cataatgcca attgcgcccg catccggaac gccgattttc ttcgccagtt
    51781 cctcaccggg atgcgcgatg agatgcgcca gtgtgacctg tttgccgggc acaaattcct
    51841 gaatgatgcg ttctttatcc atgagtcgcc gcctgtgaaa aactttcgtg tatcgtgaca
    51901 atttttcgtc agagtaaata acaaaattcg gcaagcgcgt ttaaaaggtg aggtagatca
    51961 ctaagaatgg agagatattc gctgtggtgc ataaactttc atatgcaacg catgaatata
    52021 aataaaataa tatcgagata ttatatagac tgcttttttt attattcgca aactcagtaa
    52081 taagctttac taatatattg cagccaaacc gcttaccctg aatattcagg gtaagcgtga
    52141 gagttaaaaa agaattacag cggttgggtt tgcgcttcaa ccacggccag cgccaccatg
    52201 ttgacgatac gacgcaccga tgcgatcggc gttaacacgt gaaccggttt cgccacgccc
    52261 atcagcaccg gaccgacagt cacaccttcc gagctggaaa cgcgcagtaa gttgtaacta
    52321 atgcgggcag cttccatgtt cggcatcacc agaatattgg cagaaccttt caaagggctg
    52381 tccggcatac ggtcgttgcg aatcgcttcc accagcgctg catcgccgtg catttcacca
    52441 tcaatcatca gatctggcgc acgatccctg accagttcca gcgcctgacg cattttgctc
    52501 gacgacgggc agtcagaaga accaaagttg gagtgcgaca acagagcaac gcgcggctca
    52561 ataccaaaac gacggacagt ttctgccgcc atcaaggtga tctccgccag ctcttctgcg
    52621 tctggttcgt cattgacgta ggtatcggca ataaaggtgt taccactcgg cagcagcagc
    52681 gcgttcattg cacctgcggt gtgaacgcca tcgcgataac caaagacatt tttcaccacg
    52741 ctaaaatgtt catgataatc gcccaccgta ccgcagatca ttgcatcggc ttccccacgc
    52801 tgaaccatga tcgcgccgat cactgtcggg ttactgatca gcgcccgctg cgcctgttcc
    52861 tgagtgacac cgcgacgctt catgatctgg aagtattcgg tccagtactc tttaaagcgc
    52921 ggatcggatt cgttattgac gatctcaaaa tcaacgcccg ctttgatctg caagcccagt
    52981 ttctgaatgc gcatttcgat cacgttcgga cgaccgataa ggatcggttt cgccagtccc
    53041 agcgttacca gttcctgagt ggcatgcaga acgcgcgcct cttccccttc tggcagaaca
    53101 acgcgcttcg gcgctttgcg agcctgggag aaaatcggct tcataaacag gttggttttg
    53161 taaacgaact cagtcagctt gtcgatgtag acgtcgaaat cagcaatcgg acgcgtcgcc
    53221 acgcccgact ccatcgcggc tttagcgacc gcaggagcga tcttaacgat caagcgcgga
    53281 tcaaacggtt ttggaatgat gtattccgga ccaaagctca gatcctgatc gccatacgct
    53341 gaagccacca cttcgctctg ttcggcgtgg gccagttccg caatcgcgcg taccgccgcc
    53401 agtttcatct cttcattgat agccgttgcg ccaacatcca gcgcgccacg gaagataaac
    53461 gggaagcaaa ggacgttgtt gacctggttt ggatagtcag aacgaccggt gcaaatgatg
    53521 gcatccggac gcacttcttt cgccagcggc ggcagaattt ccggttccgg gttcgccagc
    53581 gccaggatca ttggcgcacg agccattttc ttcaccattt cctgggtcag cactttcggg
    53641 ccggaacatc caaggaaaat atccgcgcct tcaatcacat catcgagggt acgtttgccg
    53701 tcatccacca ccgcatatgc ggctttggtt tccgccatgt ttggctcacg gccctgatag
    53761 ataacgcctt ttgaatcgca aaccacgatg ttatgttttt gcagacccag cgctaccagc
    53821 aggttcatac aggcgattgc tgcggcaccc gcgccggaaa ccaccatccg cacgtcggag
    53881 atgtttttct ccaccacgcg caagccgttg aggatggcgg cagtgctgat gattgccgtg
    53941 ccgtgctgat cgtcgtggaa taccggaata ttcatccgct cgcgcagttt ctgttcaata
    54001 tagaaacatt ctggcgcttt aatgtcttcg aggttgattc cgccgaaggt cggttcgagc
    54061 gcagcgacaa cttcaataaa tttgtccggg tcgagttcat caacttcaat gtcaaataca
    54121 tcaatcccgg cgaatttctt aaacagaacg cccttgcctt ccatcaccgg tttgccagcc
    54181 agcgcgccaa tgttgcctaa ccccagcacc gccgtaccgt tagagatcac cgccaccagg
    54241 ttaccgcgcg cggtatattt gtaggctttt aacgggtctt tttcgatttc aagacaaggt
    54301 gcggcaacgc ctggtgagta ggccagcgcc agatcgcgct gcgttgccag aggcttggtt
    54361 ggagaaacct ggattttccc tggaactgga aattcatgga aatcaagtgc actttgtttt
    54421 aactggtcat ccatttggtt gttcctttca cgtaacgttc acaaataaag tgtgtgggca
    54481 acagcccctg cccacaacgt ggcgcacatt attaccctgc cggagtctac agactttgag
    54541 caagtccaaa ctctcaccat taatataatg ttttggtaat aatcctataa cactgatgtt
    54601 acctgcttaa tccagcaata ccatgcctgt ctgctatgct tttttgatgc gtttagcgaa
    54661 atttctcaga agtgtgaatt aacgcactca tctaacactt tacttttcaa ggagtatttc
    54721 ctatgaacga gttagacggc atcaaacagt tcaccactgt cgtggcagac agcggcgata
    54781 ttgagtccat tcgccattat catccccagg atgccaccac caatccttcg ctgttactca
    54841 aagctgccgg attatcacaa tatgagcatt taatagacga tgctatcgcc tggggtaaaa
    54901 aaaatggcaa gacccaggaa caacaggtgg tcgcagcgtg tgacaaactg gcagtcaatt
    54961 tcggtgctga aattctcaaa atcgtacccg gtcgcgtgtc aacagaagtt gatgcacgcc
    55021 tctcttttga taaagaaaag agtattgaga aggcgcgcca tctggtggac ttgtatcagc
    55081 aacaaggcgt tgagaaatca cgcattttga tcaaactggc ttcgacctgg gaaggaattc
    55141 gcgcggccga agagctggaa aaagaaggta ttaactgcaa cctgacgctg ctgttttctt
    55201 ttgcccaggc gcgggcctgt gcagaagcgg gcgtttttct gatttcgccg tttgtcgggc
    55261 gtatttatga ctggtatcag gcacgcaagc cgatggaccc gtatgtggtg gaagaagatc
    55321 cgggcgttaa atcggtgcgc aatatctacg actactataa gcaacaccac tatgaaacca
    55381 ttgtgatggg cgcgagcttc cgtcgcaccg aacaaatcct cgccttaacc ggctgcgatc
    55441 gactgactat cgcaccgaat ttgctaaagg agctgcagga aaaagtttcg ccagtggtac
    55501 gtaaattaat cccaccttct cagacgttcc cacgcccagc tcccatgagc gaagcggagt
    55561 tccgttggga gcacaatcag gatgcgatgg cggtagaaaa actgtctgaa ggcattcgtc
    55621 tgttcgccgt tgatcaacgc aaactggaag atcttcttgc cgccaaacta taaaccagcc
    55681 acggagtgtt atatgtcccg aaaagacctt gccaatgcga ttcgcgcact cagtatggat
    55741 gcggtacaaa aagccaattc tggtcatccc ggcgcgccga tgggcatggc tgatattgcc
    55801 gaagtgctgt ggaacgattt tcttaaacat aaccctaccg acccaacctg gtatgatcgc
    55861 gaccgcttta ttctttccaa cggtcacgcg tcgatgctgc tctacagttt gctacatctg
    55921 accggttacg acctgccgct ggaagaactg aagaacttcc gtcagttgca ttcgaaaacc
    55981 ccaggccacc cggagattgg ttatacgcca ggcgttgaaa ccaccaccgg cccgcttgga
    56041 caaggtttgg cgaacgccgt cgggctggcg atagcagagc gtacactggc ggcgcagttt
    56101 aaccagccag accatgagat cgtcgatcac ttcacctatg tgtttatggg cgacggctgc
    56161 ctgatggaag gtatttccca cgaagtctgt tcgctggcag gcacgctggg actgggcaag
    56221 ctgattggtt tttacgatca caacggtatt tccatcgacg gtgaaactga aggctggttt
    56281 accgacgata cggcaaaacg ttttgaagcc tatcactggc atgtgatcca tgaaatcgac
    56341 ggtcacgatc cgcaggcggt gaaggaagcg atccttgaag cgcaaagcgt gaaagataag
    56401 ccgtcgctga ttatctgccg cacagtaatt ggcttcggtt cgcctaacaa agcgggtaag
    56461 gaagaggcgc atggcgcacc gctgggtgaa gaagaagtgg cattggcacg gcaaaaactg
    56521 ggctggcacc atccgccatt tgaaatccct aaagagattt atcacgcctg ggatgcccgc
    56581 gaaaaaggcg aaaaagcgca gcagagctgg aatgagaagt ttgccgccta taaaaaggct
    56641 catccgcaac tggcagaaga gtttacccgt cggatgagcg gtggtttacc gaaggactgg
    56701 gagaaaacga ctcagaaata tatcaatgag ttgcaggcga atccggcgaa aatcgctacc
    56761 cgtaaggctt cgcaaaatac gcttaacgct tacgggccga tgctaccgga gctgctcggc
    56821 ggttcggcgg atctggctcc cagcaacctg accatctgga aaggttctgt ttcgctgaag
    56881 gaagatccag cgggcaacta cattcactac ggggtgcgtg aatttggcat gaccgctatc
    56941 gccaacggca tcgcgcacca cggcggcttt gtgccgtata ccgcgacgtt cctgatgttt
    57001 gttgaatacg cccgtaacgc cgcgcggatg gcggcactga tgaaagcgcg gcagattatg
    57061 gtttataccc acgactcaat tggcctgggc gaagatggtc cgacgcacca ggctgttgag
    57121 caactggcca gcctgcgctt aacgccaaat ttcagcacct ggcgaccgtg cgatcaggtg
    57181 gaagcggcgg tgggctggaa gctggcggtt gagcgccaca acggaccgac ggcactgatc
    57241 ctctcaaggc agaatctggc ccaggtggaa cgtacgccgg atcaggttaa agagattgct
    57301 cgtggcggtt atgtgctgaa agacagcggc ggtaagccag atattattct gattgccacc
    57361 ggttcagaga tggaaattac cctgcaagcg gcagagaaat tagcaggaga aggtcgcaat
    57421 gtacgcgtag tttccctgcc ctcgaccgat attttcgacg cccaggatga ggaatatcgg
    57481 gagtcggtgt tgccttctaa cgttgcggct cgcgtggcgg tggaagcagg tattgccgat
    57541 tactggtaca agtatgttgg tctgaaaggg gcaattgtcg ggatgacggg ttacggggaa
    57601 tctgctccgg cggataagct gttcccgttc tttggcttta ccgccgagaa tattgtggca
    57661 aaagcgcata aggtgctggg agtgaaaggt gcctgatggt gatcgccgga tgctgattgc
    57721 cggatgcgac gctgacgcgt cttatccggc ctacagcgtc ttatccggcc tacatgtccc
    57781 cgcaattttg tttaacgggt gatccacaac gtgggccagg cgtctggccc atgccagtta
    57841 tcgcaggtgg gttctgcggc ataacgcacc aggcgaaaac gctgaccgtc aaagcgccag
    57901 cgcgcctgaa tgccacaatc gcttaatccg cgccctttcg ctaaggtcac cagttcacgc
    57961 gatttctcat caaatgttgc gttcatcagt tccagttcat tcgtctcctg accgttgttg
    58021 aacggcaaac gcaaccgaac cgggcgcgag gctagtggct ttttacgcga cacaatccat
    58081 gccaaatcaa tggtgttata ggcccctgcc tcacagctaa tcatcatcag cgctttatca
    58141 tcagtcagcg cagtgacatt cacctcacga cgtaacggat caagcgagca gcgcagacca
    58201 ttcatccgcc agtttccata atccagcaaa tcgttgcgtt cttcgagtga gagtggtgtc
    58261 ggcgttgggt taaccaccgc gacctctttc agcgcaggcg cgggcggtac gctgagcggc
    58321 ggttcgtccc ctttcttgat ccacgcggtt tcactgccaa cgcgcttttg ctgagcatca
    58381 ataaacaaca acgccgcttt taagccactc agagaaatgg tctgattgcc gtcgcgtaag
    58441 gtgatcgcct tcccttcctg aatcatctgc aaaaacgcgg tgatggttgc cgtatcatcg
    58501 gttactaata accatggtga aatccgccac ttgtcaccac ttaacgccaa cggctcacca
    58561 tctaacagca gccgtggcgc tatctctcct tcagacgcat ccggcgactt caacccgccg
    58621 cgctcaatac gtaaaacggc atcggtatgc gccccggcgc tgcggctcag ggtcatcacc
    58681 agtccattat gatcgcccgt attgcgcgcc acgcagaagt tttggttatt acaggtgacc
    58741 tgccagtcgg aaaacgcccg ttgcgctggt gccgcccaca ccaaagacgt cggcaacaaa
    58801 gcgaaaaaaa agagaaggaa aatgcgatag cgcatggacg gtacgacccc agaagttcaa
    58861 acaaacagca caagtcgtat cttcgttgcg caggtagcgc ggctcaatca gatttgtcgg
    58921 attgagccaa taataaacaa cgaaatatta ttgttatttt tcagtccatt aaatgtgacg
    58981 tttgcaaata gttaagcaat aacaccgtct taccgtcacg tatctcgccg gttttgatca
    59041 tctccagcgc ctggctgaac ggcagctcga gcacttcaat atcttcatct tcaacaccgc
    59101 caccggcgtt agcgcgctga ttgtcactgt attcggcgat aaaaaagtgg attagctcag
    59161 tcacaccgcc aggcgacata tacagttcaa ataatttgcg cacttcgccc acttcatagc
    59221 ccgtctcttc aatcgcttct ttacgaatgc acacttccgg ttcgtcgtta tccagcagcc
    59281 cggcgcaggt ttcaatcagc tgcccgcttt cattgccatt aacccaggta gcgacacgga
    59341 actgacgaat cagaaccacg ctctttttct tcgcgttgta caggaggatc gtcgcaccat
    59401 taccgcgatc gtaaacttca cgtttatggc ggataacttc gccatctttg cgcgtgagat
    59461 cgtaggtaat gttgtgcagg gtgaaatagt tatcggagag aattttgtct ttaatgaggg
    59521 tgatttgttg cgtcataccg actccacagc gcgaaatgaa caattatctt acgctgtgaa
    59581 gtcggttttg tctgcgcagg ctatcagtga gatttgactg attttacccc cagccagtta
    59641 ataatcccct gtgccgcatg acgtccttct gccattgcgg taaccaccag atccgcacca
    59701 cgcacggcgt caccaccagc gaagattttc ggattggtgg tctggtaacg gtactggctt
    59761 tccacatccg cgatgatgcg gccccatttg tctaccgtta caccgtgcga ctccagccac
    59821 ggcatcccgt gcggattgaa gccaaacgcc ataatcaccg cgtcggctgg catgacaaat
    59881 tcactgcctt ccaccggcac tggacgccga cgcccctggg catccggctc gccaagacgc
    59941 gtgcgcagga aacgaatccc gcagacgtga ccttgttcat tcagctcaag cgccaccggc
    60001 tggacgttaa attcgaagtt ggccccctct tcgcgggcgt tcttcacttc tttcttcgag
    60061 cctggcatgt tagcttcatc acgacgataa gcgcaggtga cgttactcgc gccgtggcgc
    60121 agtgcggtac gcacacagtc catcgcggtg tcgccgccgc ccagtaccac gacgttaagt
    60181 ccggcggtat tgataaacgg ctcttccggt agctcttcga gccccatcac ctgtttagtg
    60241 ttggcaatga ggaacggcag cgcgtcataa acgcccggcg catcttcatt gggtaaaccc
    60301 gctttcatgg aacggtaagt gcctacgcca acgaagaccg cgtcgtattg ttccaaaagc
    60361 gaatccaaag agacatcttt acccacttca caattgagtt cgaagtgaat ccccatcgcg
    60421 ctgaagattt cccgacggcg tgccagcagg gatttatcca gtttgaaaga aggaatgccg
    60481 aaagtgagca agccaccgat ttctggatgg cgatcgtaca ccgtcacccc cacgccattg
    60541 cgggtcagaa catccgcaca ggccagccct gccggacctg caccgataat cgccacccgc
    60601 ttgtccactt tggtgacatg gcttaagtca ggacgccaac ctttcgccaa cgcctgatct
    60661 gaaatgtagc gttcaatgtt gccgatagtt accgcgccgt gctcatcgcg aatagtacag
    60721 gcaccttcac acaaacggtc ttgcggacaa acgcgtccgg taatttccgg cagggtgttg
    60781 gtctggtgag aaagctcgac ggcggcgtcg atgtttccgg ctttcaccag ttcaatccac
    60841 tgcggtatat ggttatgcag cgggcaggtc cattcacaaa cgctgtgctc gccgcactta
    60901 aggcagcgcg aggcttcccg ttgtgcctgg tcggcgcgaa atggcagata aatttcatca
    60961 aaaccggttt tgcgcgcttc aatcgccagt ttatccggct cgccacgcgc gggcgttgcc
    61021 tgcatttgtt cgactttact cattaccggc atttcttgcg ccgcggtact ggcatgccac
    61081 ggttgatgtt cctgacgcgc ggtgcgcaag cggcgggatt tcgccatgcc ggagagtgcg
    61141 acgtcagtga ccagttgcag cgcgtccgcc gggcaattct caacacaggc aggaccgttt
    61201 tcgcgccccg cacaaaggtc gcatttatgc gccgtggctt ttacttttcc tgccgcgacg
    61261 ggtgtcagga cgatttgcat cgtaccaaaa ggacaggcca ccacgcagga tttacagcca
    61321 atacactttt gctgattgac ctgaatgctg tcatcaacgt ggctgattgc gccattaggg
    61381 cagctacggg cgcagggcgc atcttcacaa tggtgacagg tcactgcact acgttgctgt
    61441 tgatgtttga taaccgtaat tcggggatga aaatggtgtt ggctcaggac atgttgctca
    61501 tcattgtgag ccatgacaca ggcgatttca caagcatgac aacccagaca ttgctgactg
    61561 ttggccataa taaaacgatt cataacgacc ttcttttttg gttgtaaaaa ccttattctt
    61621 tatatgagtg ttgttattac ccgacttaca ggggaatcgg caatgttcat gtgcccagaa
    61681 taagtaacta tttcgctata aactgtggca gatcaaataa tcccatcact gactaaattg
    61741 tgtttcagcg aactggaaca ttaatgattt tttgtggaga agacgcgtgt gattgttaaa
    61801 cgacccgtct cggccagtct ggcccgggcc tttttttaca ttgtgctgct gtcgattctt
    61861 tccacgggta tcgctctgct aactctggcg agcagtttgc gcgacgctga ggctatcaat
    61921 attgccggat cgctacgtat gcagagttac cgcctgggct acgacttgca aagtggcagt
    61981 ccacaactca atgcacatcg ccagttgttt cagcaggcac tgcattcacc ggtattaacc
    62041 aatctcaacg tctggtatgt gccagaagca gtaaaaactc gctatgcgca tctgaatgcc
    62101 aactggctgg agatgaataa tcggctcagc aagggcgatt tgccgtggta tcaggccaat
    62161 attaataatt atgttaatca gatagacctg ttcgtgctgg ctttacagca ctacgctgaa
    62221 cgcaaaatgc tgctggtggt ggcgatttcc ctggctggcg gtatcggtat tttcacgctg
    62281 gtctttttta ctttgcgccg tatacgccat caggtggttg ccccgctgaa tcagctggtt
    62341 accgccagtc aacgtattga acacgggcag ttcgactcgc cgccgctgga taccagtctg
    62401 ccgaatgagc ttggtctgct tgcaaaaacc tttaaccaga tgtcgagcga gctgcataaa
    62461 ttgtaccttt cgctggaggc gtcagtagaa gaaaagaccc gcgatctcca cgaggccaag
    62521 cgtcgtctgg aggtgttgta tcaatgttcc caggcgctga acaccagcca gattgatgtg
    62581 cattgtttcc gccatatttt gcagattgtt cgcgacaatg aagcggctga atatctggag
    62641 ttaaatgtcg gtgaaaactg gcggattagc gaagggcaac caaacccgga attgccgatg
    62701 cagattttac cggtgacaat gcaagagacg gtttacggcg aactgcactg gcaaaatagt
    62761 cacgtttcat catcagaacc gctgcttaac agtgtttcat cgatgctggg acgcggtttg
    62821 tactttaatc aggcgcagaa gcattttcag cagttattgt tgatggaaga acgtgcaacc
    62881 atcgcccgcg aattgcacga ctcgctggct caggtacttt cttacttacg tatccagttg
    62941 acgttactga agcgttcgat accggaagat aacgccaccg cacaaagtat catggccgat
    63001 ttttcccagg cgttgaatga tgcttatcgg cagttacgcg agctgttgac tactttccgc
    63061 ctgacgctgc agcaggcgga tctcccctcc gcgttgaggg aaatgctgga tacgttacaa
    63121 aatcaaacca gcgccaaact gaccctcgac tgccgtctgc caaccctggc gctggatgcg
    63181 caaatgcagg tgcatttgtt gcaaattatt cgcgaagcgg tgctgaatgc gatgaagcac
    63241 gccaacgcca gcgaaatcgc cgtcagttgc gtcaccgcgc cggacggcaa tcacacggtt
    63301 tatatccgtg ataacgggat tggtatcggt gaaccgaaag aacccgaagg tcattatggt
    63361 ctgaatatca tgcgcgaacg cgcggaacgg ctgggtggga cgctgacttt ttcacaacct
    63421 tccggcggcg gcacgttagt gagtattagc tttcgctctg cggagggtga ggaaagtcag
    63481 ttgatgtaat gcctcctact gaccaaagaa tacttgcact taaggttcag tataaaaggg
    63541 catgataatt tacattaact cctttttttc tccacgattg gctcgtacct tgccgctaca
    63601 gtgaagcaag tcaagcctac aacgatacgc agaaacacga ggtcctcttt taatggcgaa
    63661 tttctttatt gatcgcccca tttttgcctg ggtgctggca atcctgttgt gtctgacagg
    63721 gaccctggcg attttttcat tgcccgttga acaatacccc gatctcgcgc caccgaatgt
    63781 gcgagtgacc gctaactatc ccggcgcatc ggcccagacg ctggaaaaca ccgtgaccca
    63841 ggttatcgag caaaatatga ccggcctcga taatctcatg tatatgtcat ctcagagcag
    63901 tggcaccggt caggcatctg tcactttaag ttttaaagca ggcaccgatc cggacgaagc
    63961 cgtgcagcaa gtacaaaacc agctgcaatc agccatgcga aagttaccgc aggcggtgca
    64021 aaatcagggc gtgacggtgc gtaaaaccgg cgataccaat attctgacca ttgctttcgt
    64081 ctctaccgat ggttcgatgg ataaacagga tattgccgat tatgttgcca gtaatattca
    64141 ggacccgtta agccgcgtga atggcgtcgg ggatatcgat gcctatggtt cgcaatattc
    64201 catgcgtatc tggctggacc cggcgaaact caacagtttc cagatgacgg ctaaagatgt
    64261 cactgatgcc attgagtcac agaacgcgca gattgcggtt gggcaacttg gtggtacacc
    64321 ttccgtcgat aagcaggcgc tcaacgccac cattaacgcc cagtcactgc tgcaaacacc
    64381 agaacagttc cgcgatatca ccttgcgggt gaatcaagac ggctcagagg taaggctggg
    64441 cgatgtcgcc accgtcgaaa tgggggcgga gaaatacgat tatcttagcc gcttcaatgg
    64501 taagccagcc tccgggctgg gggtaaaact ggcctccggc gcaaacgaaa tggcgacagc
    64561 ggagctggtg ctcaatcgtc tcgacgagct ggcgcagtac ttcccgcacg gactggaata
    64621 caaggtggcg tatgaaacca cctcgtttgt taaagcctcc attgaagacg tggtgaaaac
    64681 gctgctggaa gctatcgctc tggttttcct cgttatgtat ctgttcctgc aaaacttccg
    64741 cgccacgctg atacccacta tcgccgtgcc ggtggtgttg atgggaacct tctccgtact
    64801 ttacgccttt ggttacagcg tcaacacctt aaccatgttc gcgatggtgc tggcgatcgg
    64861 tctgctggtg gatgacgcca tcgtggtggt ggaaaacgtc gaacgtatta tgagtgagga
    64921 aggactcact cctcgcgaag ccacacgtaa atcgatgggg cagatccagg gggcactggt
    64981 cgggattgcg atggttcttt cggcggtatt tgtaccaatg gccttcttcg gcggcaccac
    65041 cggtgccatc tatcgccagt tctctattac cattgttgcg gcgatggtgc tgtcagtact
    65101 ggtagcgatg atcctcactc cggctctgtg tgccacacta cttaagccac tgaaaaaagg
    65161 tgagcatcac gggcaaaaag gcttttttgc ctggtttaac cagatgttta accgcaacgc
    65221 cgaacgctac gaaaaagggg tggcgaaaat tctccaccgt agcctgcgct ggattgtgat
    65281 ttatgtcctg ctgcttggcg gcatggtgtt cctgttcctg cgtttgccga cgtcgttctt
    65341 accgctggaa gaccgtggca tgtttactac ctcggtacaa ttgcccagcg gttcaaccca
    65401 acaacagacc ctgaaagtcg ttgagcaaat cgagaaatat tacttcaccc atgaaaaaga
    65461 caacatcatg tcggtgtttg ccaccgttgg ttctggccct gggggcaacg ggcaaaacgt
    65521 ggcgagaatg tttatccgcc tgaaagactg gagcgaacgc gacagtaaga ccggcacctc
    65581 gtttgccatt atcgagcgtg caacgaaggc gtttaaccaa attaaagaag ctcgcgttat
    65641 cgccagcagc ccgccagcaa ttagcggtct tggtagttct gcgggttttg atatggagtt
    65701 gcaggaccac gctggagcag gtcacgatgc gctgatggca gcacgtaacc agttgctggc
    65761 gctggcggcg gaaaacccga agctaacccg tgtgcgccat aacggcctcg atgacagtcc
    65821 gcagttgcag attgatatcg accagcgtaa agctcaggcg ctgggcgttg ctatcgacga
    65881 tattaacgac acactgcaaa ccgcctgggg ttcgagctat gtgaatgact ttatggatcg
    65941 cggtcgcgtg aagaaagtct atgtgcaggc agctgcgccg tatcgcatgc tgccagatga
    66001 cattaatctc tggtatgtcc gaaataaaga tggcggcatg gtgcccttct ctgctttcgc
    66061 aacctcacgc tgggagacag gctcaccgcg tctggaacgc tataacggtt attctgcggt
    66121 tgagattgtt ggagaagccg caccgggggt cagtaccggt acggcgatgg atattatgga
    66181 atcgttagtg aagcagttgc caaatggctt tggtctggag tggacggcga tgtcgtatca
    66241 ggagcggctt tccggcgcgc aggctccggc gctgtacgcc atttccttgc tggtagtatt
    66301 cctgtgtctg gcggcattgt atgaaagctg gtcggtaccg ttctcggtaa tgctggtcgt
    66361 gccgctgggg gtaatcggcg cgctgctggc aacctggatg cgcgggctgg aaaacgacgt
    66421 ttacttccag gtgggcctgt taacggtcat tggtttatcg gcgaaaaacg ccatcctgat
    66481 cgtctagttt gctaacgaga tgaaccaaaa aggccacgac ctgtttgaag cgacgctcca
    66541 cgcctgccgt cagcgtttac gcccgattct gatgacctcg ctggcattta tcttcggcgt
    66601 attgccaatg gcaaccagca cgggtgccgg ttccggtggt cagcatgcgg tgggtactgg
    66661 cgtaatgggc gggatgattt cagctactat tctggctatt tacttcgtgc cgctgttctt
    66721 tgtgctggtg cgccgccgct tcccgctgaa gccgcgcccg gaataagcaa taaaaaaggc
    66781 gacatgccaa tgtgtcgcct ttttcaactt tccgattaag aacctgctca gcgggttctt
    66841 gctgtttata ctttgtctcg ggaattactt acgaagcata acttcgataa agtctttcca
    66901 gttccccagt tcacgttcaa tcataacaac ctctcttata attatgggta ttctacggaa
    66961 acaatatacc gtggtgaagc taatttactc gattgctgcg atgactacct ccgggggaca
    67021 aaccttatgt aaatactatg gtcctgcggt gataatttgt atgtgataca cagcaacatt
    67081 tcgagatatt catacggcat ctaatactga tttaattctg gttaaaatac agacag
//
LOCUS       DAZFQD010000009        66532 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.009, whole genome shotgun sequence.
ACCESSION   DAZFQD010000009 DAZFQD010000000
VERSION     DAZFQD010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 66532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 66532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66532
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.009"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..1716)
                     /gene="rsxC"
                     /locus_tag="ACHKTW_000635"
     CDS             complement(<1..1716)
                     /gene="rsxC"
                     /locus_tag="ACHKTW_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HFO6461710.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQSNGTPLRQVPLAQRFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWADYRTRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSNDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPADLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKAARLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAAREARKAQARAKQAELQQTNDAATVADPRKTAVEAAIARA
                     KARKLE"
     gene            complement(1709..2287)
                     /gene="rsxB"
                     /locus_tag="ACHKTW_000636"
     CDS             complement(1709..2287)
                     /gene="rsxB"
                     /locus_tag="ACHKTW_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HFO6461711.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRAMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            complement(2287..2868)
                     /gene="rsxA"
                     /locus_tag="ACHKTW_000637"
     CDS             complement(2287..2868)
                     /gene="rsxA"
                     /locus_tag="ACHKTW_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HFO6461712.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLASICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            complement(2945..3385)
                     /gene="ydgK"
                     /locus_tag="ACHKTW_000638"
     CDS             complement(2945..3385)
                     /gene="ydgK"
                     /locus_tag="ACHKTW_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HFO6461713.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEDGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            complement(3471..3686)
                     /gene="ydgT"
                     /locus_tag="ACHKTW_000639"
     CDS             complement(3471..3686)
                     /gene="ydgT"
                     /locus_tag="ACHKTW_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HFO6461714.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            complement(3959..4084)
                     /gene="blr"
                     /locus_tag="ACHKTW_000640"
     CDS             complement(3959..4084)
                     /gene="blr"
                     /locus_tag="ACHKTW_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HFO6461715.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSASVQHK"
     gene            4327..5367
                     /gene="ydgJ"
                     /locus_tag="ACHKTW_000641"
     CDS             4327..5367
                     /gene="ydgJ"
                     /locus_tag="ACHKTW_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFO6461716.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            complement(5402..6403)
                     /gene="add"
                     /locus_tag="ACHKTW_000642"
     CDS             complement(5402..6403)
                     /gene="add"
                     /locus_tag="ACHKTW_000642"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009168 - purine ribonucleoside
                     monophosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFO6461717.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARHGLHYVELRFSPG
                     YMAMAHQLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(6507..7679)
                     /gene="malY"
                     /locus_tag="ACHKTW_000643"
     CDS             complement(6507..7679)
                     /gene="malY"
                     /locus_tag="ACHKTW_000643"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HFO6461718.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDTQTVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADDWFCDMGKLEAVLAK
                     PECKIMLLCSPQNPTGKVWTCDELEIMADLCERHGVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYISDKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            complement(7689..9281)
                     /gene="malX"
                     /locus_tag="ACHKTW_000644"
     CDS             complement(7689..9281)
                     /gene="malX"
                     /locus_tag="ACHKTW_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HFO6461719.1"
                     /translation="MTAKTAAKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESATRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVKDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            9456..10484
                     /gene="malI"
                     /locus_tag="ACHKTW_000645"
     CDS             9456..10484
                     /gene="malI"
                     /locus_tag="ACHKTW_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HFO6461720.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLSGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGTTLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            10596..11363
                     /gene="hdhA"
                     /locus_tag="ACHKTW_000646"
     CDS             10596..11363
                     /gene="hdhA"
                     /locus_tag="ACHKTW_000646"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HFO6461721.1"
                     /translation="MFNSDNLRLDGKCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLGGQAFACRCDITSEQELSALADFAVSKLGKVDILVNNAGGGGPK
                     PFDMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     SSKAAASHLVRNMAFDLGEKNIRVNGIAPGAILTDALKSVITPEIEQKMLQHTPIRRL
                     GQPQDIANAALFLCSPAASWVSGQILTVSGGGVQELN"
     gene            11592..12182
                     /gene="uidR"
                     /locus_tag="ACHKTW_000647"
     CDS             11592..12182
                     /gene="uidR"
                     /locus_tag="ACHKTW_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HFO6461722.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTSMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            12571..14382
                     /gene="uidA"
                     /locus_tag="ACHKTW_000648"
     CDS             12571..14382
                     /gene="uidA"
                     /locus_tag="ACHKTW_000648"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HFO6461723.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRA
                     IAVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRIFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     KKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     gene            14379..15752
                     /gene="uidB"
                     /locus_tag="ACHKTW_000649"
     CDS             14379..15752
                     /gene="uidB"
                     /locus_tag="ACHKTW_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
                     /protein_id="HFO6461724.1"
                     /translation="MNQQLSWRTIVGYSLGDVANNFAFAMGALFLLSYYTDVAGVGAA
                     AAGTMLLLVRVFDAFADVFAGRVVDSVNTRWGKFRPFLLFGTAPLMIFSVLVFWVPTD
                     WSHSSKVVYAYLTYMGLGLCYSLVNIPYGSLATAMTQQPQSRARLGAARGIAASLTFV
                     CLAFLIGPSIKNSSPEEMVSVYHFWTIVLAIAGMVLYFICFKSTRENVVRIVAQPSLK
                     ISLQTLKRNRPLFMLCIGALCVLISTFAVSASSLFYVRYVLNDTGLFTVLVLVQNLVG
                     TVASAPLVPGMVARIGKKNTFLIGALLGTCGYLLFFWVSVWSLPVALVALAIASIGQG
                     VTMTVMWALEADTVEYGEYLTGVRIEGLTYSLFSFTRKCGQAIGGSIPAFILGLSGYI
                     ANQVQTPEVIMGIRTSIALVPCGFMLLAFVIIWFYPLTDKKFKEIVVEIDNRKKVQQQ
                     LISDITN"
     gene            15791..17056
                     /gene="uidC"
                     /locus_tag="ACHKTW_000650"
                     /pseudo
     CDS             15791..17056
                     /gene="uidC"
                     /locus_tag="ACHKTW_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
     gene            complement(17101..18609)
                     /gene="ydgA"
                     /locus_tag="ACHKTW_000651"
     CDS             complement(17101..18609)
                     /gene="ydgA"
                     /locus_tag="ACHKTW_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HFO6461725.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLK
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLASFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATEFMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYTNGQITLNGQKMPLEDFVGMFAMPALNVPVVPAIPQQ"
     gene            complement(18710..19885)
                     /gene="manA"
                     /locus_tag="ACHKTW_000652"
     CDS             complement(18710..19885)
                     /gene="manA"
                     /locus_tag="ACHKTW_000652"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416130.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009298 - GDP-mannose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HFO6461726.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     RNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPNAERLSELFASLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            20084..21730
                     /gene="fumB"
                     /locus_tag="ACHKTW_000653"
     CDS             20084..21730
                     /gene="fumB"
                     /locus_tag="ACHKTW_000653"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFO6461727.1"
                     /translation="MSNKPFHYQAPFPLKKDDTEYYLLTSEHVSVSEFEGQEILKVAL
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGCDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            21873..23276
                     /gene="fumC"
                     /locus_tag="ACHKTW_000654"
     CDS             21873..23276
                     /gene="fumC"
                     /locus_tag="ACHKTW_000654"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045239 - tricarboxylic acid cycle
                     heteromeric enzyme complex [Evidence IEA];
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HFO6461728.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLGLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGR"
     gene            complement(23273..24202)
                     /gene="tus"
                     /locus_tag="ACHKTW_000655"
     CDS             complement(23273..24202)
                     /gene="tus"
                     /locus_tag="ACHKTW_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006274 - DNA replication termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HFO6461729.1"
                     /translation="MARYDLVDRLNTTFRQMEQELATFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(24278..25579)
                     /gene="rstB"
                     /locus_tag="ACHKTW_000656"
     CDS             complement(24278..25579)
                     /gene="rstB"
                     /locus_tag="ACHKTW_000656"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HFO6461730.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLVQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALAMGGTVNCDTSELGGARFSFSWPLWHNIPQFTSA"
     gene            complement(25583..26302)
                     /gene="rstA"
                     /locus_tag="ACHKTW_000657"
     CDS             complement(25583..26302)
                     /gene="rstA"
                     /locus_tag="ACHKTW_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HFO6461731.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     SPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWE"
     gene            26431..26766
                     /gene="ydgC"
                     /locus_tag="ACHKTW_000658"
     CDS             26431..26766
                     /gene="ydgC"
                     /locus_tag="ACHKTW_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HFO6461732.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            complement(26763..27485)
                     /gene="folM"
                     /locus_tag="ACHKTW_000659"
     CDS             complement(26763..27485)
                     /gene="folM"
                     /locus_tag="ACHKTW_000659"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HFO6461733.1"
                     /translation="MGKAQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLIKAGAQCIQADFSTNDGVMAFADEVLKSTHGLRAILHNASAWMAEKPGTPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHVAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(27522..28904)
                     /gene="ydgI"
                     /locus_tag="ACHKTW_000660"
     CDS             complement(27522..28904)
                     /gene="ydgI"
                     /locus_tag="ACHKTW_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFO6461734.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            complement(29090..30034)
                     /gene="ydgH"
                     /locus_tag="ACHKTW_000661"
     CDS             complement(29090..30034)
                     /gene="ydgH"
                     /locus_tag="ACHKTW_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YdgH"
                     /protein_id="HFO6461735.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQERGNNLTVSADLYK"
     gene            30558..32090
                     /gene="pntA"
                     /locus_tag="ACHKTW_000662"
     CDS             30558..32090
                     /gene="pntA"
                     /locus_tag="ACHKTW_000662"
                     /EC_number="7.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HFO6461736.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYTVPGEIFTTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLASVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            32101..33489
                     /gene="pntB"
                     /locus_tag="ACHKTW_000663"
     CDS             32101..33489
                     /gene="pntB"
                     /locus_tag="ACHKTW_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HFO6461737.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTQMLFGD
                     AKASVDAILKAL"
     gene            complement(33514..34548)
                     /gene="tqsA"
                     /locus_tag="ACHKTW_000664"
     CDS             complement(33514..34548)
                     /gene="tqsA"
                     /locus_tag="ACHKTW_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HFO6461738.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTRTLPQYRNSIM
                     TPLQALEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            34960..35325
                     /gene="mdtJ"
                     /locus_tag="ACHKTW_000665"
     CDS             34960..35325
                     /gene="mdtJ"
                     /locus_tag="ACHKTW_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015606 - spermidine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015848 - spermidine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HFO6461739.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            35312..35641
                     /gene="mdtI"
                     /locus_tag="ACHKTW_000666"
     CDS             35312..35641
                     /gene="mdtI"
                     /locus_tag="ACHKTW_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HFO6461740.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVAYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(35680..36501)
                     /gene="ydgD"
                     /locus_tag="ACHKTW_000667"
     CDS             complement(35680..36501)
                     /gene="ydgD"
                     /locus_tag="ACHKTW_000667"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HFO6461741.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYDIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKSAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTDDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(36601..36684)
                     /locus_tag="ACHKTW_000668"
     CDS             complement(36601..36684)
                     /locus_tag="ACHKTW_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6461742.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            complement(36777..37085)
                     /gene="asr"
                     /locus_tag="ACHKTW_000669"
     CDS             complement(36777..37085)
                     /gene="asr"
                     /locus_tag="ACHKTW_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance repetitive basic protein Asr"
                     /protein_id="HFO6461743.1"
                     /translation="MKKVLGLVVAAAMGLSSAAFAAETATTPAPTATTTKAAPAKTTH
                     HKKQHKAAPAQKAQAAKKHHKNTKAEQKAPEQKAQAAKKHAGKHSHQQPAKPAAQPAA
                     "
     gene            37356..37460
                     /locus_tag="ACHKTW_000670"
     CDS             37356..37460
                     /locus_tag="ACHKTW_000670"
                     /inference="COORDINATES: protein motif:HMM:NF033695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KPN_01571 family protein"
                     /protein_id="HFO6461744.1"
                     /translation="MNPFIWVLIILMTLDALRELAGASSILGWLLTLV"
     gene            complement(37509..38762)
                     /gene="ynfM"
                     /locus_tag="ACHKTW_000671"
     CDS             complement(37509..38762)
                     /gene="ynfM"
                     /locus_tag="ACHKTW_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO6461745.1"
                     /translation="MSRTTTVDGAPASDTDKQSISQPNQFIKRGTPQFMRVTLALFSA
                     GLATFALLYCVQPILPVLSQEFGLTPANSSISLSISTAMLAIGLLFTGPLSDAIGRKP
                     VMVTALLLASICTLLSTMMTSWHGILIMRALIGLSLSGVAAVGMTYLSEEIHPSFVAF
                     SMGLYISGNSIGGMSGRLISGVFTDFFNWRIALAAIGCFALASALMFWKILPESRHFR
                     PTSLRPKTLFINFRLHWRDRGLPLLFAEGFLLMGSFVTLFNYIGYRLMLSPWHVSQAV
                     VGLLSLAYLTGTWSSPKAGTMTTRYGRGPVMLFSTGVMLFGLLMTLFSSLWLIFAGML
                     LFSAGFFAAHSVASSWIGPRAKRAKGQASSLYLFSYYLGSSIAGTLGGVFWHNYGWNG
                     VGAFIALMLVIALLVGTRLHRRLHA"
     gene            38869..39762
                     /gene="ynfL"
                     /locus_tag="ACHKTW_000672"
     CDS             38869..39762
                     /gene="ynfL"
                     /locus_tag="ACHKTW_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO6461746.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPHDHPLANNPNVTLAELAKEPFVFFDPHVGTGLYDDILGLMRRYHL
                     TPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSEMWLVWP
                     KHHEQSPAARNFRIHLLNALR"
     gene            39897..41117
                     /gene="mlc"
                     /locus_tag="ACHKTW_000673"
                     /gene_synonym="dgsA"
     CDS             39897..41117
                     /gene="mlc"
                     /locus_tag="ACHKTW_000673"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar metabolism global transcriptional
                     regulator Mlc"
                     /protein_id="HFO6461747.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVQELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDSPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGAGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIITGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            41242..41937
                     /gene="bioD"
                     /locus_tag="ACHKTW_000674"
     CDS             41242..41937
                     /gene="bioD"
                     /locus_tag="ACHKTW_000674"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFO6461748.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKTVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TEKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(41890..43182)
                     /gene="clcB"
                     /locus_tag="ACHKTW_000675"
     CDS             complement(41890..43182)
                     /gene="clcB"
                     /locus_tag="ACHKTW_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HFO6461749.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAAFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSPWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAVVALLVSNLINHSDAL
                     LYNVQLSVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVGLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(43341..43955)
                     /gene="dmsD"
                     /locus_tag="ACHKTW_000676"
     CDS             complement(43341..43955)
                     /gene="dmsD"
                     /locus_tag="ACHKTW_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HFO6461750.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            complement(43998..44852)
                     /gene="ynfH"
                     /locus_tag="ACHKTW_000677"
     CDS             complement(43998..44852)
                     /gene="ynfH"
                     /locus_tag="ACHKTW_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate reductase membrane anchor subunit YnfH"
                     /protein_id="HFO6461751.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQRIV
                     RGMFFLWLLMGIGFIASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAGVIVLQGLSLASIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLILGLILILGGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            complement(44854..45408)
                     /locus_tag="ACHKTW_000678"
     CDS             complement(44854..45408)
                     /locus_tag="ACHKTW_000678"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMSO/selenate family reductase complex B
                     subunit"
                     /protein_id="HFO6461752.1"
                     /translation="MVSNNFKDLGPEVSFRRIYEYAGGDWQEDNGVWHQNVFAYYLSI
                     SCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGCRYCHMACPYGAPQYNAEKGHM
                     TKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQKHGTLAAVAPLPRAHFTKPNI
                     VIKPNANSRPTGDTTGYLANPEEV"
     gene            complement(45446..46609)
                     /locus_tag="ACHKTW_000679"
     CDS             complement(45446..46609)
                     /locus_tag="ACHKTW_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO6461753.1"
                     /translation="MSLFRRGEIWYASYSLPGGKRIKESLGTKDKRQAQELHDKRKAE
                     LWRVDRLGDMPDVTFEEACLRWLEEKADKKSIDSDKSRIAFWIEHFEGIRIKDISEAM
                     IYSVISKAYNRKTKERWKLQVEAALRKGKEPPAYIPKSVSTQTKATHLAMIKAILRAA
                     ERDWKWLEKAPVIKIPAVKNKRVRWLEKEEARRLIDACSDPLKSVVKFALATGLRRSN
                     IINLEWQQIDMQRRVAWVNPEDSKSNRAIGVALNDTACKVLRDQIGKHHRWVFVYTTA
                     ARRPDGTMTPSIRKMRLDYNTSWLTACRRAGIENFRFHDLRHTWASWLIQSGVPLSVL
                     QEMGGWESIEMVRRYAHLAPNHLTEHARKIDDILSDDVPNLSHPEVFEDAKKA"
     gene            complement(46741..46836)
                     /locus_tag="ACHKTW_000680"
                     /pseudo
     CDS             complement(46741..46836)
                     /locus_tag="ACHKTW_000680"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(46871..47122)
                     /locus_tag="ACHKTW_000681"
     CDS             complement(46871..47122)
                     /locus_tag="ACHKTW_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFO6461754.1"
                     /translation="MFVLIQRGQSFVDANNYPVEICKVTLTQVIYRRLDGRTRATSIG
                     AFNEEFERVDHNELHMIKAEIEKEMHIASLRKMRRTSIN"
     gene            complement(47170..47850)
                     /locus_tag="ACHKTW_000682"
     CDS             complement(47170..47850)
                     /locus_tag="ACHKTW_000682"
                     /EC_number="3.1.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lambda exonuclease family protein"
                     /protein_id="HFO6461755.1"
                     /translation="MTPEIILQRTGIDVRGVEQGDYAWQKLRLGVITASEVHNVIAKP
                     RSGKKWPDMKMSYFHTLLAEVCTGVAPEVNAKALAWGKQYENDARALFEFTSGVNITE
                     SPIIYRDESMRTACSPDGLCSDGNGLELKCPFTSRDFMKFRLGGFEAIKSAYMAQVQF
                     SMWVTRKDAWYFANYDPRMKREGLHYVVVERDEKYMAGFDEMVPEFIEKMDEALAEIS
                     FVFGEQWR"
     gene            complement(47847..48632)
                     /gene="bet"
                     /locus_tag="ACHKTW_000683"
     CDS             complement(47847..48632)
                     /gene="bet"
                     /locus_tag="ACHKTW_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage recombination protein Bet"
                     /protein_id="HFO6461756.1"
                     /translation="MSTALATLAGKLAERVGMDSVDPQELITTLRQTAFKGDASDAQF
                     IALLIVANQYGLNPWTKEIYAFPDKQNGIVPVVGVDGWSRIINENQQFDGMDFEQNDE
                     SCTCRIYRKDRNHPICVTEWMDECRRAPFKTREGREITGPWQSHPKRMLRHKAMIQCA
                     RLAFGFAGIYDKDEAERIVENTAYTTERQPERDITPVNEETMSEINALLTSMEKTWDD
                     DLLPLCSQIFRRYIRASSELSQAEAEKVLGFLKQKATEQKVAA"
     gene            complement(48638..48934)
                     /locus_tag="ACHKTW_000684"
     CDS             complement(48638..48934)
                     /locus_tag="ACHKTW_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HFO6461757.1"
                     /translation="MNAWLIPDRIEEQSWARHYRQITREENETELADDLEKGLPQHLF
                     ESLCIDNLQRHGASKKAIARAFDDDVDFQERMAEHIRYMVETIARHQIYIDSEV"
     gene            complement(48931..50976)
                     /locus_tag="ACHKTW_000685"
     CDS             complement(48931..50976)
                     /locus_tag="ACHKTW_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001271580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051908 - double-stranded DNA 5'-3' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0090304 - nucleic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecE family exodeoxyribonuclease"
                     /protein_id="HFO6461758.1"
                     /translation="MSADKQTFALHCEAKNDKVRKRLGIKGGFFWADTRKLSVAVSRC
                     IAAMDDAGYDEDDFKKPVRVNFPVVNDLPPEGVFDTEFCNRYEKGGEDGITMMAIPFD
                     DNINGEDATTAGDDYDNLDGTIPDDVEKSESPDSDDDCSECEIPVATLSLTHRFLHLF
                     FSKDEDGKYRHHATPEQRNNVIRMEMDTEDSYLQSLLTAVRSHPELDKLTNYHLGRLA
                     QSVGKAFSHATPRHISPAEFDKFISTWMKTDYVDQGLLAKEWQKGNYVTGITRTPSGA
                     NAGGGNLTDRGEGFTHNQASLARDIATGVLARSMDVDIYNLHPAHAKRVEEIVAENKP
                     PFSVFRDKFIAMPGGLDYSRAIVVASVKEAPIGIEAIPARVTEYLNKVLTETDHSNPD
                     PEIVEIACGRSSAPMPQRGTAEEIHDDEEKQQTSDAMSNEQAAPESVEEIPVKHNADT
                     QSLENVSSVETKYQELRAELYEARKNILPKNPVDADKLLAASRGEFVEGISDPTDPKW
                     VKGIQTRDTEDQNQSKVEQNSPDTQQNGPEEQQPGPVMQQEVEKVCTACGQNSGGHCP
                     DCGPVMGDETYAETFGENETAAGDDQAQMKNEYIQKNAVDTTQEHDSVVHIDPDSDAS
                     DNNDDSEQADFDWKRQLVLAAVYGLCANPACVATAPAIPDIAIMIANRLENFGGDKS"
     gene            complement(51085..51471)
                     /locus_tag="ACHKTW_000686"
     CDS             complement(51085..51471)
                     /locus_tag="ACHKTW_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6461759.1"
                     /translation="MKELAQNEFFSETSPDAINELKEIAERISKICKKHKIDFVFSFS
                     VLTEVGNNEYKDSRFVSFGLYCETTSPYIHAASEIVDSHIDPQRIQELTLALQLLREN
                     GECNCSDCQHEKKKTTHKTTNQATLH"
     gene            complement(51562..51777)
                     /gene="kil"
                     /locus_tag="ACHKTW_000687"
     CDS             complement(51562..51777)
                     /gene="kil"
                     /locus_tag="ACHKTW_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560215.1"
                     /note="Rac prophage Kil protein; Kil overexpression leads
                     to morphological defects and inhibits cell division;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFO6461760.1"
                     /translation="MIAHHFGTDEIPRQCVKPGDYVLHEGRTYIASANNIEKRKLYIR
                     SLTTKTCITDCMIKVFIGRDGLPVKAE"
     gene            complement(52109..52465)
                     /locus_tag="ACHKTW_000688"
     CDS             complement(52109..52465)
                     /locus_tag="ACHKTW_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001005965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6461761.1"
                     /translation="MNETELKHIIALLLEDARQVYRLSPNSATQERIRMAEKALKQES
                     ENNISEVSANGENEAIEINSNISNGVIASSHKTILMGARLLEVITSELEKNGIKPTPC
                     YLECVLNVIAYSMFRR"
     gene            complement(52497..53210)
                     /locus_tag="ACHKTW_000689"
     CDS             complement(52497..53210)
                     /locus_tag="ACHKTW_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000211196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6461762.1"
                     /translation="MTTPSVLPQKLWRPLAEIKNFVEKMPDGVRLTEVTKKVKTFAEL
                     SGKERNQLIDFIDKRESIIVFKVRKEGSGNGVTFFRHKKYGYPKREGNVTIIKDLQSK
                     LCTRCGQTKSVDDFYSDASKRDGRAIYCKKCESAMKRSRRECNKLILQQQEPEMNNLK
                     AVSPSPEILRKQAEELLKAAEIAEKKRQEDDVFNKKLAPLKLEILQAAGKMQLKLDEF
                     IDCMDEMNKAVQKLKELTA"
     gene            complement(53265..53336)
                     /locus_tag="ACHKTW_000690"
     CDS             complement(53265..53336)
                     /locus_tag="ACHKTW_000690"
                     /inference="COORDINATES: protein motif:HMM:NF022510.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction Ral family protein"
                     /protein_id="HFO6461763.1"
                     /translation="MVWAIRTTAMIARELSKQKNKAA"
     gene            complement(53564..53902)
                     /locus_tag="ACHKTW_000691"
     CDS             complement(53564..53902)
                     /locus_tag="ACHKTW_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000930321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO6461764.1"
                     /translation="MLMVKKTIYVNPNRGQNRKVSDRSLTSRDRRRIARWEKRIAYAL
                     ENGITPGFNAIDDGPEYKINEDPMDKVDKALATPFPRDVEKIKDEKYEDIMHRVVNHA
                     HQRNPNKKWS"
     gene            complement(53871..54209)
                     /locus_tag="ACHKTW_000692"
     CDS             complement(53871..54209)
                     /locus_tag="ACHKTW_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
                     /protein_id="HFO6461765.1"
                     /translation="MVTIIWKESKGTAKSRYKARREELIAERRSSERLTRKIALKISG
                     CVRADKAASLGSLRNKKAEEVERKNRSTYYKDSNPLGNKIHAVQKIKLYSKPPYGLIE
                     YAYGEKDYLC"
     gene            54555..54764
                     /locus_tag="ACHKTW_000693"
     CDS             54555..54764
                     /locus_tag="ACHKTW_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000502039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6461766.1"
                     /translation="MLIEGEFGVLHQRVVEILGVSLLEVIATGEAISADAIAGMIRVL
                     HHDELDDLAVKLAIDVLLQDMRLCN"
     gene            complement(54917..55624)
                     /locus_tag="ACHKTW_000694"
     CDS             complement(54917..55624)
                     /locus_tag="ACHKTW_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000846395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFO6461767.1"
                     /translation="MKTFAERLNAAMSSAGLSQAQLADMVGVSQPAIQKMSSGKTTGS
                     RKMVELANALRVRPEWLSSGIGSMRPQSAEEPSNARESSLKAIAWDDHQNDNDEFVAL
                     PLLNISLSAGGGSCALEESAEFSLVFRRYYLKKMGVPEKAAKLVRVVGQSMEPTLHDG
                     DVVGVNTQDTSIRDGKTYAICQADLLRVKTLIATPTSVIIRSINREEYPDEVMNREEF
                     YKNVKIIGRVFWSSHSW"
     gene            55735..55959
                     /locus_tag="ACHKTW_000695"
     CDS             55735..55959
                     /locus_tag="ACHKTW_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001054988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO6461768.1"
                     /translation="MNLVIQRALNIVGSQKRLAADCGVSQPAVHKWLRGGKVSPEKVF
                     AIVNATNGQVKAYEIRPDLPHLFPHPNQAE"
     gene            56075..56371
                     /locus_tag="ACHKTW_000696"
     CDS             56075..56371
                     /locus_tag="ACHKTW_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CII family transcriptional regulator"
                     /protein_id="HFO6461769.1"
                     /translation="MSQTSYSKLSQRDIDRAETDLLINLSVLTQRGLAKMIGCHESKV
                     SRTDWRYIAAILCAFQMASDISPISRAFQHAIRVHANKKRPVGAGRSEQILMNI"
     gene            56386..56514
                     /locus_tag="ACHKTW_000697"
     CDS             56386..56514
                     /locus_tag="ACHKTW_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000438870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6461770.1"
                     /translation="MEQYTGIILPHLEEFRQQQHQPQQPEADWVNPGDTGTVCGME"
     gene            56535..57617
                     /locus_tag="ACHKTW_000698"
     CDS             56535..57617
                     /locus_tag="ACHKTW_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001390692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO6461771.1"
                     /translation="MRNYATISPQFWLGDTGRKLRKSGPECMVVALYMMTSPHSNMLG
                     LYYLPVLYIAHETGLDPEGASKGLQMACEAGFCSYDHDSEVVWVHEMAAWQVGEALKP
                     GDNRCAGVRNEYSALLENPFLSSFYDRYKDDFHLDVRRESCRKIEAPSEPLSSQEQEQ
                     EQEQERDKTLLVHGEKIATDPLVDSCPVLTERPGPAGMTPEADSGRCVQQVLHVEPEQ
                     QHQPQQPEADSAMSGKPIGLTRAMPGPSAGRVDYPDVFERVWREYPHRAGSNPKKSAF
                     NAWRARLREGVSPDVVLDGVRRYARYLEATGKAGTEFVQQASTFFGPNRNFENPWSLP
                     KAGAVSLRCVNHISEPDTEIPPGFRG"
     gene            57624..58360
                     /locus_tag="ACHKTW_000699"
                     /pseudo
     CDS             57624..58360
                     /locus_tag="ACHKTW_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000789014.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            58386..59156
                     /locus_tag="ACHKTW_000700"
     CDS             58386..59156
                     /locus_tag="ACHKTW_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001403602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFO6461772.1"
                     /translation="METVLHALKAMGKANSVELAARLDISREEVLNELWELKKNGVVD
                     KTGHTWFLAVEGESGVTEGQALQSEAPDVVTEEVAPKVSADMMIEFIAQEGAKTCEEI
                     AGKFGVTTRKVASTLAVVTATGRLVRVSIKGKFRYKVPDVENGDLSASSSQDENITHE
                     AENSASGDELNRFPLMSEGTAKACTDEFIRDVPSFTEKYAGAVTLPPLRAASRELRRA
                     KKQVQKWERVCAALRELNKHRELVMQITDSSSSIASEK"
     gene            59171..59602
                     /locus_tag="ACHKTW_000701"
     CDS             59171..59602
                     /locus_tag="ACHKTW_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFO6461773.1"
                     /translation="MAKVFTPEQREELKARIVELVRLNGRGTFRQLATETGVSKTAVR
                     RLSRELVVSGAIFDSGYGLFPSDQARKDWQNARKKLSRAKAKKPVVIDPGLIRALPDG
                     EIRRYDRRYNIICRECRNSETMQRVLAFWRGSAEGLMFSPS"
     gene            complement(59635..60309)
                     /locus_tag="ACHKTW_000702"
     CDS             complement(59635..60309)
                     /locus_tag="ACHKTW_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4145 domain-containing protein"
                     /protein_id="HFO6461774.1"
                     /translation="MSLVSFDITCPHCLKERAVIEAFAEKQIEKTPLFNVAFVCRSCH
                     RGGVAIVEIPSDHYHGPMAESQKKDLDILISGNSQYRFRKIYPAVKKITAPEYTPPVA
                     DRTFVEAKEDLQRRRYDTVVILCRRVLDISTKKLLGDEAGKESLSQRIQMIYKKGLIT
                     EQMKEWAHIVRIDANKAVHTDEVFTPIEASQILSFTEMFLVYAFTLPAMVAARREQKR
                     SDSASI"
     gene            60308..60553
                     /locus_tag="ACHKTW_000703"
     CDS             60308..60553
                     /locus_tag="ACHKTW_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6461775.1"
                     /translation="MDIPLEIKEINEIIEVTDRPEFTLMRRYETGTDEQKYIMVAALA
                     VMAIERERREKDVMVIPARNDSPDLRWQDPEWDVAIR"
     gene            complement(60601..60906)
                     /locus_tag="ACHKTW_000704"
     CDS             complement(60601..60906)
                     /locus_tag="ACHKTW_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001240641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFO6461776.1"
                     /translation="MAMFNPPHPGEIIADILEDQNIGIRELARALDVAPSTVQRLVSG
                     NATISPEMAVRLAAVLGGTPSSWIRLQTAWSLEKAEREVDVSHLSTKYRPAEISPHA"
     gene            complement(60906..61184)
                     /locus_tag="ACHKTW_000705"
     CDS             complement(60906..61184)
                     /locus_tag="ACHKTW_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005188495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO6461777.1"
                     /translation="MIKSWKHKGLQRFFETGNASGVNPNHVKRLRTRLQVIDQAEIID
                     DINLAGYRLHQLQGDRSDTWSVTVSGNWRITFEFRNGDAYILNYEDYH"
     gene            61519..61830
                     /locus_tag="ACHKTW_000706"
                     /pseudo
     CDS             61519..61830
                     /locus_tag="ACHKTW_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232082.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
     gene            62099..62761
                     /locus_tag="ACHKTW_000707"
     CDS             62099..62761
                     /locus_tag="ACHKTW_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HFO6461778.1"
                     /translation="MTMQLAVESLVVVTYSNVPVITTGLLANLYKTEEKHIRQNFKRN
                     ECRFIAGKHFFKVSGCELDSLRTSQRGLQISPKTRSLILWTERGAARHAKMLETDQAW
                     EVFEKLEDCYFSQGKTTQTEQQSQIQPQFTAEEIILLCYMQLWMEKAQDLSKHLYPIM
                     KELNSSYTNKLYDITFETIYMVTKNRDALLREAARLDQSSFVVQRARPMLKSLRARQF
                     EF"
     gene            63016..63660
                     /locus_tag="ACHKTW_000708"
     CDS             63016..63660
                     /locus_tag="ACHKTW_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000107186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BRO family protein"
                     /protein_id="HFO6461779.1"
                     /translation="MSTQLVFKSHVLETIEHDGKQWFTAATLASALEYSRGDKITQIY
                     NRNSDEFTPCMSINLKMRFNGINNSLREKDVRIFSLRGAHLVAMFATTPVAKEFRRWV
                     LDILDREAEKSAIPPQPHIQPQFTAEDIILLCYVQLWMEKAQQLSKQLYPVMRELNSD
                     YYGKLFDLAYETRHITNRTRDTLLREAAKLDPANYMVHRARSMLAQLRARQFEF"
     gene            63715..64125
                     /locus_tag="ACHKTW_000709"
     CDS             63715..64125
                     /locus_tag="ACHKTW_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinB"
                     /protein_id="HFO6461780.1"
                     /translation="MNQTIFLRSKQQQQFAINAILATTLDKDKPVTIRITDYKRNLDQ
                     NAKFHAMVADISRQVQWCGRWLKPEQWKVLLISGHAVATKQEADVLPGLEGECVNIRE
                     SSAQMSVKRMASLIEYTTSWAVEKGVRFTDRRYE"
     gene            64122..64313
                     /locus_tag="ACHKTW_000710"
     CDS             64122..64313
                     /locus_tag="ACHKTW_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001254268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HFO6461781.1"
                     /translation="MRRQRRSITDIVCENCNYLPTKRSRNKPRPIPNESDVKTFNYTS
                     HLWDVRWLRRRARFNSHSG"
     gene            64337..64627
                     /locus_tag="ACHKTW_000711"
     CDS             64337..64627
                     /locus_tag="ACHKTW_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000002235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HFO6461782.1"
                     /translation="MANLRKEARGRECQVRIYGVCNGNPETTVLAHYRIAGICGTGMK
                     PDDLLAAWACSDCHNEIDRRTRILDNNDARLYHLEGVIRTQAILLKEGKIKS"
     gene            64624..64986
                     /locus_tag="ACHKTW_000712"
     CDS             64624..64986
                     /locus_tag="ACHKTW_000712"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001008184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFO6461783.1"
                     /translation="MNEYEFVLPWPPTVNTYWRRRGSQYYISDKGQKYRKDVQQIIRQ
                     LRLDIFTKSRLRITIIAEPPDSRRRDLDNILKGLLDSLIYAGFAEDDEQFDDIRVIRG
                     VKVPGGRVGIKITELENI"
     gene            64986..65180
                     /locus_tag="ACHKTW_000713"
     CDS             64986..65180
                     /locus_tag="ACHKTW_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000144764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ninH"
                     /protein_id="HFO6461784.1"
                     /translation="MNAKIQTIPELLICTRGNQTEVARILNCNRATVRKYIDDKDAKK
                     HAVVNGVLMVHRGWGKDTDA"
     gene            65173..65553
                     /locus_tag="ACHKTW_000714"
     CDS             65173..65553
                     /locus_tag="ACHKTW_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFO6461785.1"
                     /translation="MRDIRQVLERWGAWAANNYEDVTWSPIAAGFKGLIPEKVKSRPQ
                     CCDDDAMIICGCMARLNRNNSDLHDLLVDYYVLGETFMALARKHGCSDGTIGKKLQKA
                     EGVVEGMLMLLGVRLEMDRYVQRL"
     gene            65686..66279
                     /locus_tag="ACHKTW_000715"
     CDS             65686..66279
                     /locus_tag="ACHKTW_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000350575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6461786.1"
                     /translation="MDIEDFLEAEDNDLSLWNDDILSIDWFVILRELLKYQRRLNITH
                     SELVLLANLVSFHQNVDSQILPSISLLATRMRASRAIIQGRLQELEGKGMLQKISYAQ
                     IGHDDDLRNVYDIKPLIRKLIGVQKTENDKKHTCPVCGKVGSSDEEIEKFFGFRKSGD
                     YKWPQSWCRSCRSTKQRRLNTSLIGKKRPRKSPPEVV"
BASE COUNT        16826 a        16071 c        16860 g        16775 t
ORIGIN      
        1 ttccagcttg cgcgctttgg cgcgggcgat agctgcttca acggcagttt tacgtggatc
       61 agcaacggtt gctgcgtcgt tagtttgctg cagttctgcc tgtttcgctc tggcttgcgc
      121 tttacgggct tcccgtgctg caataattgc actgttatcc gggcgttcgc ccgctttaat
      181 cacaataggc tgtgtagcct gggcctgttt ctctttcacc cgcgccagag cggcagcaat
      241 cgcatcttta tctttggctg caggttgaac ggctgcgctc ttatgtcgtt caaggcgagc
      301 cgctttttcg cgctccagac gagcctggcg cgcttcgaaa cgcgctttgg cttctgcggc
      361 gcgcttttct tcctgacgaa tagccgcaat ttcagctttt tcctgacgga aatattgcac
      421 caggggaata ttgctcgggc aaacccacgc gcaagcccca cattcaatgc aatcagcaat
      481 gttatgcgtg gtagctttat cgtgttgctg acctttgctg aaccagtaca actgttgcgg
      541 caaaagatcc gcagggcagg cgtcagcaca ggcgctacac cggatgcagc tttgttcttc
      601 ctgtggttcg ccaagttcat tggcagaggg agccaacaga cagttggtaa tctttacgac
      661 cgggacatcc agccatggca aggtaaagcc cattagcggg ccacccataa tcaccatttg
      721 atcggcagag gggcagaatc cggcatcatt caataaatga cgcactggcg tccccagccg
      781 tgcccagacg ttgcccgggc gagcgattgc ttcgccagtc agggttacaa cacgctcggt
      841 aatcggctcg ccatcaataa cggcacgttt cactgcataa gcagtgccga cgttttgcat
      901 taatacgccg atatcggatg aacgcccgcc atgtggaacc tgcttcccgg tcagaatgta
      961 ggttaattgt ttagcaccgc cagaaggata tttggttgga atcacccgca gagaaatatc
     1021 gttagagtcc gccagcaccg cgcgcagcat ggaaatcgcc tgcggtttgt tatcttcaat
     1081 gccgataaga atttcgcgtg gctgcagaat atgcgcaaga atgcgaatac cctctacgac
     1141 ctgagccgcg caatcctgca tcaaacggtc atcggcggta atgtacggct cgcactcagc
     1201 cgcgttgata atcaacgttt caatcttatc tccgccaccc tgcaatttaa cgcctgtcgg
     1261 gaatcctgca ccgcccagcc cggctacacc aaactgatgt atgcgctcga ttaactcttc
     1321 gcgactgcga gtgcgataat cggcccagcc gtcgcgcggg atccagcagt cttcaccatc
     1381 ggcatcaata atcacgctta attcagctaa agctgaagga tgagccgtag agtggggcgc
     1441 aatagccgta acggtacccg aggtgggcgc gtgaacaggc agcattttgc cgcgaccacg
     1501 ggtaagcggc tggccgcgca atactttatc gccgacgcta acgcacaact caccttcagc
     1561 gccaatatgc tgtttcagtg gaataacaaa acgctgcgcc agtggtacct ggcgcagggg
     1621 tgtaccgttg gactgggttt tcatctccgg tggatggatg ccgccgttga aatcccagat
     1681 tttatttttt ctgaatgcag agaataactt aagcatggtg ttccacggga atgatacgca
     1741 cgggaatggt gttcagatcc catttccagg agtcaggtgt ttctgcgacc ggttgcaacg
     1801 agatgcagtg cgtcgggcac ggatcaacac ataaattgca gcccgtacag agatcactca
     1861 ttaccgtatg catggcacgg gtagcgccaa cgatggcgtc taccggacaa gcctgaatac
     1921 atttagtgca gccaatacag ttattttcat caataaccgc caccatccgc gcaggcgtta
     1981 gctcttgcgc ttcgccatcc agcggctgcg gctcgacatt aagcaactcg gcaattttta
     2041 gcatcacagc ttcgccacct ggggcgcaac ggttgatttt ttcaccgtta cagctgatgg
     2101 cttccgcgta ggggcgacag ccgggataac cgcactgacc acactggctc tgcggtaaga
     2161 tttcgtcaat tttctcaacg accggatcgt cttccaccgc aaaacggcgg gaggcataac
     2221 ccagaatggc gccaaacgcc aggcccagca ggctcacggc ggcaacggca atccagatag
     2281 cattcattac aacttcacca aaccactaaa gcccataaag gccagagaca taagacctgc
     2341 ggtaattaac gcaatggcat taccgcgaaa aggtgccggg acatcagcca cagcaaggcg
     2401 ttcgcggatg gcggcgaaga gcaccatcac cagcgagaaa ccgacagcgg cggaaaaacc
     2461 gtacagcgcc gactgcaaga aattgtgccc gagattgata ttcagcaacg ccacgccgag
     2521 cactgcacag ttggtggtga taagcggcaa aaaaatcccc agcagacggt aaagcaccgg
     2581 gctggttttg cgcaccacca tctcggtgaa ctgcacgacc acagcaatca ccagaataaa
     2641 tgccagggtg cgcaggtaaa tcagattaag tgggatcaaa atccacgtat cgataagcca
     2701 ggcgcaaata gacgccagcg tcatcacaaa cgttgttgcc agccccatgc ccatcgcggt
     2761 ttccagtttt ttggaaaccc ccataaacgg acagagaccg agaaacttga ccagtacaaa
     2821 gttattgacc agtacagttc cgacaaacag taacaggtag tcagtcattt ttcggcctga
     2881 aataaaaaag ccgcctatta tccgttaatc cagagcaggc gacaacaggt taactgtaag
     2941 gttattacgg attcacaaac gtcgctttta cccgcgacga gcgtttgaaa taggggacga
     3001 tcaatgcggt tgccaacaaa gtaaacagca actggcgaac ggctatgcca tcttccacgg
     3061 gtgaaaaagc gaatgctttt actgccagca atacggaaat aagcagccag ataatatagt
     3121 gtttaggaac acaacgccga cgtttaaaga aagcgatggt caaccacagt gtgtaatacc
     3181 acatggcgat agcggtaata aaagaaacgc cccataagat ttgtgtcgtc agtgcttgtc
     3241 cgccgagcgt ttgaaatgtt tgcggagaag ataacgcagc ggtatacagc aacaacgcca
     3301 gcgtcgtact caacagagcg actaaaagcc aggccaacgg gccaagcaac cagcctccaa
     3361 tacgttgtgg cgttgtggtg gtcataggtt ctccagaata gcgacgcaaa caggcaaatc
     3421 taattttagg ggcgttatta taaggctttt ataaccgatc gctactttct ttattggaca
     3481 tagtgccaga cggacttcgg tacctggccg aggtcaaaca aacgcccgcc agaaaccagc
     3541 tctgcgcgac ggtgatcggc agcacgatac atattgatca gttcctgatc gtccgtcagg
     3601 gtgtaattaa gatggtcgta gagtttttcc agactttcga gtgaactgat tttgcgaaat
     3661 tttaataagt agtcctgaac agtcataaaa ggtccataaa ataattaata ccaaaggggt
     3721 aggtattatt agtgatgata aaatttcacc aaaagtgaac gcttgtgtta ttacaataat
     3781 aagaatgcgc tgatggcaaa tattcaggga tgaaaaactg ctctgaaaag agtaacgcgc
     3841 tttactatcc ctgacaatca ccaacaacat cgaacaagat aataaattcc tggtttaata
     3901 tccgacaagt gaaaacatgc acccggacgg gcagcatgtc gctccacaag tgcagagctt
     3961 acttgtgttg taccgaagca ctctgttcag gtggctgata gttgtcaatg tgactcgcca
     4021 cgccaagaag aatgactgaa acgacaagaa cgatccaacc tgttaattca ataagacgat
     4081 tcattacagc ccacatctct cttgattgat ccattaactt cagggggtaa atgttactta
     4141 gcaataatag ctccagcaag atttttactg aggtttttgc gatatttagc ttttgtcgtt
     4201 ggaaaattcg ctattttttt gacttaagtt aaacaacatc ccttattgct ggcaggttat
     4261 taaactgttg agcgtgggta aaggatagtg tcaaatagcc atcatacttc aatgagaggc
     4321 aatgacatga gcgacaacat ccgtgttggg ttgattgggt atggttatgc gagcaaaacc
     4381 ttccatgcgc ccctgattgc gggcacgccc gggctggaac tggcggtaat ctccagcagt
     4441 gatgaaacaa aagtaaaagc cgactggcca acggttacgg ttgtctctga gccgaagcat
     4501 ctgtttaacg atcccaacat agacctgatt gtcattccta cacccaacga tacccatttc
     4561 ccgttagcca aagcggcgct tgaggcgggt aaacatgtgg tcgttgataa accctttacc
     4621 gtgacactgt cacaagcgcg agagctggat gcgctggcaa aaagcctggg gcgtgtgctg
     4681 tctgtattcc ataaccgtcg ctgggatagc gatttcttga cgctaaaagg tttactcgcg
     4741 gaaggcgtgc tgggtgaagt tgcttacttt gagtctcatt ttgaccgctt ccgtccgcag
     4801 gtgcgcgatc gttggcgtga acagggcggc ccaggcagcg gtatctggta cgatttagca
     4861 ccacatcttc ttgatcaggc cattacgctt tttggtttac cggtcagcat gacggtagat
     4921 ttggcacagt tacggcccgg agcgcagtcg accgattatt tccacgccat cttgtcctat
     4981 ccacagcggc gagtcatttt acacggtacc atgctggcag ccgctgagtc agcacggtat
     5041 atcgtgcatg gatcccgagg cagttatgtg aaatatggcc tcgatccaca ggaagaacgt
     5101 ctgaaaaatg gcgagcgtct accgcaggaa gactggggct acgatatgcg tgatggcgta
     5161 cttacccgcg tggaaggtga ggaacgtgtc gaagaaacgc tgttgacggt gcctgggaat
     5221 tatccggctt actatgcggc tattcgtgat gcgttaaatg gcgatggtga aaatccggtt
     5281 ccggcaagcc aggcaatcca ggtaatggag ttgattgagc tgggcatcga atccgccaaa
     5341 catcgcgcga ctttgtgcct tgcatgaaaa atggatggtg caatttgcac catccatttt
     5401 gttacttcgc ggcgactttt tctcgcagtg cgcgtttttc ctcagcgctg aggaaagcca
     5461 tttccagacc attaatctgt gcctggcgga tttgctcgcg ggataaccca gcagctggcg
     5521 cggcaacggt atattcgtga atgatatcca ctccctgtac gccgggatcg tcagtgttaa
     5581 tgctggcacg aatgccatgc tcaaggaacg ttttcagcgg atgtgcagcc agctctgcta
     5641 cggtgctggt ctgaatattg gaggtcagac aggattcaat accaatttgt tgctcggcga
     5701 gaaaatccat cagcgcccga tcttcaatgg cttttacgcc atgtccaata cgctccgcac
     5761 ccagttcacg aatcgcctgc cagatgcttt ccggcccggc agcttcgcct gcatggacgg
     5821 taatatgcca gcccgcatca cgcgcgcggt tgaagtgaga aaggaacaga cttcccggga
     5881 aaccaagttc atcaccggct aaatcaagtg cggtaatctg gtcacggtgg gctaaaaagg
     5941 cctccagctc ttgctgacag gcggcttcgc cgaaggtccg gctcataatg ccgataagct
     6001 tcgcctgcac accaaaggtg cggcaacctt cacgtacgcc atcgatcacc gcttcgacaa
     6061 cacccgctac aggcagctga tgtgccattg ccatgtagcc tggtgaaaaa cgcagctcga
     6121 catagtgcag gccgtgacgg gctgcatctt caatgttttc aaatgccacg cggcgacagg
     6181 catcaagaga ggcgagaact ttaacgcccc agtcaagttt ggtcagaaag ctcaccagat
     6241 cgggttcgtt ggcaatgacc tgaacgtggg gaatcagtgt ttccagggat tgtgcaggaa
     6301 gcgagatatt atactggcgg ccaagttcaa gaatggtctg gggacgaatg ttgccatcaa
     6361 ggtggcgatg gatatcagtt aatggcaggg tggtatcaat catggtcgca ctctttttct
     6421 gaatataaaa gtgcgcacca ttataaaaac aaagcagggt aaaaatccac gattgcgcaa
     6481 cgatttttta cgttgcgcaa ttggggttag cgaacagcgc ggatggcgtt tattaatcca
     6541 gccacacctt tttccagttt cgaacgtggg cagccggcat tgagacggac aaaaccacga
     6601 ccttcttcac cataggtata ccccggcatg atcgcgactt tttcttgctc gataagcgct
     6661 ttttgcaacg cgttgtcgtc aatattcaac ggacgtagat caagccaggc cagataagtg
     6721 gattgcggga tctgccagtt gagttcagga aacgcggcgt tcattttatc tgagatatac
     6781 gtcaggttat ctttcagata gacgcgtaag gcatccagcc acggtgcacc ttgctgatag
     6841 gcggcgatat gggcagttaa cgccagtacc gaaggggaag aaagcccatc acgtcctttc
     6901 agtgccgata aataggcatc acggctgctg ctattttcta taatcccgta agcaccggtc
     6961 agcgcgggaa tattgaaact tttcgagccc gacgtcagca acgcccagtc tccccgcgcc
     7021 acattactcc agggaatatg cggctgctcg ccccagacca tatccatatg gatttcatcg
     7081 gaaataaccc gcacaccatg acgctcgcac aggtcagcca tgatctccag ctcatcgcac
     7141 gtccacactt tcccggtagg attctgtggg ctacacagga gcataatttt acattctggt
     7201 ttcgccaaca cggcttccag cttgcccata tcgcaaaacc agtcgtcagc ctgcttctct
     7261 aaagcaacgg gcattactgt gcgctggtta ccttcaatgg ccttgtaaaa tgcgtcatag
     7321 gcgggcgtgt ggatcaccac gccttcacct gtttcagacc actgacgaat cagttctgaa
     7381 accatataga tgacagaagg gccatacacc accgtctgcg tatcaattgc ggtgtaatgc
     7441 tgggtggaaa accagtgggc aatagccgcg agaaactcat cgtttttcca gcggctgtag
     7501 ccaaatacgc cgtgcatcag gcgctgattc agcgcctcga taatgcaggg ggcagtggca
     7561 aaatccatgt ctgaaatcgt gaacggtaac aggtcagcag tgccgaaacg gtcagcgaca
     7621 taatcccact gtgtacacca tgtgccatga cgatccacga cctttgaaaa atcgaacata
     7681 tcttatcctt atgcctggac agtatgcatc agaccggcca tttcatcttt tactgactga
     7741 acttgtggcc cgataacaac ctgcaggtta tgttgattaa gttgtactac gccaattgcc
     7801 cgattgtcct tcagtgcctg cacattaaca agcgacatat ctttcacaga caaacgcaga
     7861 cgggtaatgc agttatcgag gctgacaata ttgtcggcac cgcctaatgc ttcgaggatt
     7921 gcaggaacgt tgtaacctga tttacccggc gcaccggcaa cggctttttc gattgagctg
     7981 gcaacttcgc tatcgcgccc cggggttttc agattgaagc gggtgatagc gaaacggaag
     8041 atgacgtagt aaacgacaaa ccagattgcc gccaccactg gcaccatgta ccacttggtt
     8101 gacagaccat gcaaaatacc gaacaccacg aagtcgatga tattgccgtc ggtattaccg
     8161 atggtgacgc cgagcacaga catgacggtg aagccgaggc cggttaacag cgcgtggatg
     8221 acatacagaa ctggcgctac gaacaggaac aggaattcca gcggttcggt agtgccgcca
     8281 acgacgcagg cgatcaggcc agaaatcagc agacctttaa ttttatggcg attttccggg
     8341 cgcgcgcagt gatacatagc taacgctgca cccggcagac cgccgagaaa cgcaggcatt
     8401 ttaccttgcg aaaggaaacg cgtggcgctt tcagaaaaac cgtgagtggt cgggcaactc
     8461 aattgcgcct ggaagatggt cagtgcgccg ctgacggttt gaccgcagac ttcctgcgtg
     8521 ccgcctgcgt cggtaaagcg aattaatgct accagaatgt gatgcagacc aaacggcaac
     8581 agcagacgtt caccggtacc aaacagcatc ggtccgaaat cacccgcgct gtttatcata
     8641 tggcccaagc cgctaatacc catggcgaaa atcggccaga ctaatggaat caccaggccg
     8701 acaaggccca tcaccagcga ggagataatt ggtacgaagc gcgtaccgcc gaagaatgcc
     8761 agcgcatccg gcaggcggat attatggaaa cgctcatgca gcatccagac gataataccg
     8821 gcgatcaccg caccgaggat cccggtatcg atcgactgga tcccaagaat gctctggatg
     8881 ttattggctt tcagaaccgc ggcatccgtg gttggcagaa tgcctttatt ggtcaaccag
     8941 aagtttaccg cgaggttcat taccgcataa ccgacgaagc cagcgaatgc cgctacgcct
     9001 ttattttcgc gtgccaggcc cagcgggatg gcgatacaga acatgacagg caggaaacta
     9061 aaagcaaacg agccaatctt actcatccag gtaaagatag cctgcaacac ggggttgccc
     9121 aggaccggga tcagggttat gacatcatgg ctgctaagag aactaccaat gccgagcata
     9181 atgccgcaga acgacaataa tgccacgggt aacatgaagg ttttgcctaa ctgctggaag
     9241 aactcccaca gcgtgacttt cgctgctgtt ttcgccgtca taaaacgact cctcgtagag
     9301 aacaatctgg attcagtaaa ttgcgcgaga agataaaacg ttttatcaaa ttttagtgag
     9361 gcataaatca cattacgcaa cgataatagc gggtataaga taaataaaag gtaaaacgtt
     9421 ttatctgtca cataatcagg gagtaggtca tctgcatggc taccgccaaa aaaataacca
     9481 ttcatgatgt tgcgctggct gcgggcgtgt cggtaagtac cgtttcgctg gtgcttagtg
     9541 gcaaagggcg aatctctacc gccacaggag aacgcgttaa cgccgccatt gaagagctgg
     9601 gatttgtgcg caatcgccag gcgtcggcgc tgcgcggcgg gcaaagcggc gtcattggtt
     9661 tgatcgtccg tgatttatct gcgccgtttt acgccgaatt gacggccgga ttgacggaag
     9721 ctctggaagc gcagggacgg atggtttttt tgcttcacgg cggtaaagac ggcgagcagc
     9781 tggcacagcg gttttcactg ttactgaatc agggtgtgga tggtgtggta attgccgggg
     9841 ctgcaggaag cagcgatgac ctgcgacgga tggcagaaga aaaagctatc ccggtgatat
     9901 tcgcttcccg tgccagttat cttgatgatg ttgatacggt tcgcccggac aacatgcagg
     9961 ctgcacagtt gttgacggag catctcattc gcaatgggca tcagcggatc gcctggctgg
    10021 gagggcaaag ttcctcatta acccgtgcag aacgggtggg gggctattgt gcaactctac
    10081 taaaatttgg cctgccgttt cacagcgatt gggtgctgga gtgcacatcc agccagaagc
    10141 aagccgcgga agctatcacg gcgcttttac gtcataaccc gaccatcagt gctgtggttt
    10201 gctataacga aactattgcg atgggggcat ggtttggttt gctcaaagca gggcggcaaa
    10261 gcggggaaag cggagtcgat cgttactttg agcaacaagt ttcgctggcg gcatttaccg
    10321 atgcgacacc aaccacactt gatgatatac ccgttacctg ggccagcacg cctgcgcggg
    10381 aacttggtac cacacttgcg gatcgcatga tgcaaaaaat cacccatgaa gagacgcatt
    10441 cacgcaatct tattattccc gcccggctca ttgcggcgaa ataatcctct ctttatctgc
    10501 tatacctggt agtgtccttt cctcaaggtt aatgataaaa aatgtggcac acaggcggtg
    10561 ccttttattt ttactgaagt tcacaggagg tttatgtgtt taattctgac aacctgagac
    10621 tcgacggaaa atgcgccatc atcacaggtg cgggtgcagg tattggtaaa gaaatcgcca
    10681 ttacattcgc gacagctggc gcatctgtgg tggtcagtga tattaacgcc gacgcagcta
    10741 accatgttgt agacgaaatt caacaactgg gtggtcaggc atttgcctgc cgttgtgata
    10801 ttacttccga acaggaactc tctgcactgg cagactttgc cgtcagtaag ctgggtaaag
    10861 ttgatatcct ggttaacaac gccggtggcg gtggtcctaa accgtttgat atgccaatgg
    10921 cagattttcg ccgtgcttat gaactgaatg tgttttcttt tttccatctg tcacaacttg
    10981 ttgcgccaga aatggaaaaa aatggcggtg gcgttattct gaccatcact tctatggcgg
    11041 cagaaaataa aaatataaac atgacttcct atgcatcatc taaagctgcg gccagtcatc
    11101 tggtcagaaa tatggcattt gacctgggtg aaaaaaatat tcgggtgaat ggcattgcgc
    11161 cgggggcaat attaaccgat gccctgaaat ccgttattac accagaaatt gaacaaaaaa
    11221 tgttacagca cacgccgatc agacgtctgg gccaaccgca agatattgct aacgcggcgc
    11281 tgttcctttg ctcgcctgca gccagctggg taagcggaca aattctcacc gtctccggtg
    11341 gtggggtaca ggagctcaat taatacacta acgaaccggt aaacagccgt atgtgttgtt
    11401 taccgggata gcgtgtgcaa ttcatcaacg tctctgctaa acaactggca gccaaatcac
    11461 ggctattggt taaccaatag ctggatgaaa agcatacgaa tagagtgtgc cttcgcacta
    11521 ttcaacagca atgataggcg ctcacctgac aacgcggtaa actagttatt cacgctaact
    11581 ataatggttt aatgatggat aacatgcaga ctgaagcaca accgacacgg acccggatcc
    11641 tcaatgctgc cagagagatt ttttcagaaa atggatttca cagtgcctcg atgaaagcca
    11701 tctgtaaatc ctgcgccatt agtcccggga cgctctatca ccatttcatc tccaaagaag
    11761 ccttgattca ggcgattatc ttacaggacc aggagagggc gctggcccgt ttccgggaac
    11821 cgattgaagg gattcatttt gttgactata tggtcgagtc tattgtctct ctcacccatg
    11881 aagcctttgg gcaacgggcg ctggtggtcg aaattatggc ggaagggatg cgtaacccac
    11941 aggtcgccgc catgcttaaa aataagcata tgacgatcac tgaatttgtt gcccagcgga
    12001 tgcgtgatgc ccagcaaaaa ggcgagataa gcccagacat caacacgtca atgacttcac
    12061 gtttactgct ggatctgacc tacggtgtac tggccgatat cgaagcggaa gatctggcgc
    12121 gtgaagcgtc gtttgctcag ggattacgcg cgatgattgg cggtatctta accgcatcct
    12181 gatcctctca tttcgcgggc tggtgataac tgtgcccgcg tttcatactt taatttccct
    12241 gtacaaaata caatctagcc gggatcggag aattcccccc caaaaatatt caccgtagcc
    12301 atatgtcatg agagtttatc gttcccaata tactcgaacg aacgttcggt tgcttatttt
    12361 atggcttctg tcaacgccgt tttaaagatt aatgcgatca atatcacgct gtgggtattg
    12421 cagtttttgg ttttttgatc gcggtgtcag ttctttttat ttccatttct cttccatggg
    12481 tttctcacag ataactgtgt gcaacacaga attggttaac taatcagatt aaaggttgac
    12541 cagtattttt atcttaatga ggagtccctt atgttacgtc ctgtagaaac cccaacccgt
    12601 gaaatcaaaa aactcgacgg cctgtgggca ttcagtctgg atcgcgaaaa ctgtggaatt
    12661 gatcagcgtt ggtgggaaag cgcgttacaa gaaagccggg caattgctgt gccaggcagt
    12721 tttaacgatc agttcgccga tgcagatatt cgtaattatg cgggcaacgt ctggtatcag
    12781 cgcgaagtct ttataccgaa aggttgggca ggccagcgta tcgtgctgcg tttcgatgcg
    12841 gtcactcatt acggcaaagt gtgggtcaat aatcaggaag tgatggagca tcagggcggc
    12901 tatacgccat ttgaagccga tgtcacgccg tatgttattg ccgggaaaag tgtacgtatc
    12961 accgtttgtg tgaacaacga actgaactgg cagactatcc cgccgggaat ggtgattacc
    13021 gacgaaaacg gcaagaaaaa gcagtcttac ttccatgatt tctttaacta tgccgggatc
    13081 catcgcagcg taatgctcta caccacgccg aacacctggg tggacgatat caccgtggtg
    13141 acgcatgtcg cgcaagactg taaccacgcg tctgttgact ggcaggtggt ggccaatggt
    13201 gatgtcagcg ttgaactgcg tgatgcggat caacaggtgg ttgcaactgg acaaggcact
    13261 agcgggactt tgcaagtggt gaatccgcac ctctggcaac cgggtgaagg ttatctctat
    13321 gaactgtgcg tcacagccaa aagccagaca gagtgtgata tctacccgct tcgcgtcggc
    13381 atccggtcag tggcagtgaa gggcgaacag ttcctgatta accacaaacc gttctacttt
    13441 actggctttg gtcgtcatga agatgcggac ttgcgtggca aaggattcga taacgtgctg
    13501 atggtgcacg accacgcatt aatggactgg attggggcca actcctaccg tacctcgcat
    13561 tacccttacg ctgaagagat gctcgactgg gcagatgaac atggcatcgt ggtgattgat
    13621 gaaactgctg ctgtcggctt taacctctct ttaggcattg gtttcgaagc gggcaacaag
    13681 ccgaaagaac tgtacagcga agaggcagtc aacggggaaa ctcagcaagc gcacttacag
    13741 gcgattaaag agctgatagc gcgtgacaaa aaccacccaa gcgtggtgat gtggagtatt
    13801 gccaacgaac cggatacccg tccgcaaggt gcacgggaat atttcgcgcc actggcggaa
    13861 gcaacgcgta aactcgaccc gacgcgtccg atcacctgcg tcaatgtaat gttctgcgac
    13921 gctcacaccg ataccatcag cgatctcttt gatgtgctgt gcctgaaccg ttattacgga
    13981 tggtatgtcc aaagcggcga tttggaaacg gcagagaagg tactggaaaa agaacttctg
    14041 gcctggcagg agaaactgca tcagccgatt atcatcaccg aatacggcgt ggatacgtta
    14101 gccgggctgc actcaatgta caccgacatg tggagtgaag agtatcagtg tgcatggctg
    14161 gatatgtatc accgcatctt tgatcgcgtc agcgccgtcg tcggtgaaca ggtatggaat
    14221 ttcgccgatt ttgcgacctc gcaaggcata ttgcgcgttg gaggtaacaa gaaagggatc
    14281 ttcactcgcg accgcaaacc gaagtcggcg gcttttctgc tgcaaaaacg ctggactggc
    14341 atgaacttcg gtgaaaaacc gcagcaggga ggcaaacaat gaatcaacaa ctctcctggc
    14401 gcaccatcgt cggctacagc ctcggtgacg tcgccaataa cttcgccttc gcaatggggg
    14461 cgctcttcct gttgagttac tacaccgacg tcgctggcgt cggtgccgct gcggcgggca
    14521 ccatgctgtt actggtgcgg gtattcgatg ccttcgccga cgtctttgcc ggacgagtgg
    14581 tggacagtgt gaatacccgc tggggaaaat tccgcccgtt tttactcttc ggtactgcgc
    14641 cgttaatgat cttcagcgtg ctggtattct gggtaccgac cgactggagc catagcagca
    14701 aagtagtgta tgcatatttg acctacatgg gcctcgggct ttgctacagc ctggtgaata
    14761 ttccttatgg ttcacttgct accgcgatga cccaacaacc acaatcccgc gcccgtctgg
    14821 gcgcggctcg cgggattgcc gcttcattaa cctttgtctg cctggcattt ctgataggac
    14881 cgagcattaa gaactccagc ccggaagaga tggtgtcggt ataccatttc tggacgattg
    14941 tgctggcgat tgccggaatg gtgctttact tcatctgctt caaatcgacg cgtgagaatg
    15001 tggtacgtat cgtggcgcag ccgtcattga agatcagtct gcaaaccctg aaacggaatc
    15061 gcccgctgtt tatgttgtgc atcggtgcgc tgtgtgtgct gatttcgacc ttcgcggtca
    15121 gcgcctcgtc gttgttctac gtgcgctatg tgttaaatga taccgggctg ttcactgtac
    15181 tggtactggt gcaaaacctg gttggtactg tggcatcggc accgctggtg ccggggatgg
    15241 tcgcgaggat cggtaaaaag aataccttcc tgattggcgc tttgctggga acctgcggtt
    15301 atctgctatt cttctgggtt tccgtctggt cactgccggt ggcgttggtt gcgttggcca
    15361 tcgcttcaat tggtcagggc gttactatga ctgtgatgtg ggcactggaa gctgataccg
    15421 tagaatacgg tgaatacctg accggcgtgc gaattgaagg gctcacctat tcactattct
    15481 catttacccg taaatgcggt caggcaatcg gaggttcaat tcctgccttt attttgggat
    15541 taagcggata tatcgccaat caggtgcaaa cgccggaagt tattatgggc atccgcacat
    15601 caattgcctt agtaccttgc ggatttatgc tactggcatt cgttattatc tggttttatc
    15661 cgctcacgga taaaaaattc aaagaaatcg tggttgaaat tgataatcgt aaaaaagtgc
    15721 agcagcaatt aatcagcgat atcactaatt aatattcaat aaaaataatc agaacatcaa
    15781 aggtgcaact atgagaaaaa tagtggccat ggccgttatt tgcctgacgg ctgcctctgg
    15841 ccttacctct gcttatgcgg cgcaactggt tgacgatgaa gcgggactac gcatcagact
    15901 gaaaaacgaa ttgcgcaggg cggataagcc cagtgctggc gcgggaagag atatttacgc
    15961 atgggtacag ggaggattgc tcgatttcaa tagtggttat tattccaata ttattggcgt
    16021 tgaaggcggg gcatattatg tttataaatt aggtgctcgt gctgatatga gtacccggtg
    16081 gtatcttgat ggtgataaaa gttttggctt tgccctgggg gcagtaaaaa taaaacccag
    16141 tgaaaatagc ctgcttaaat taggtcgctt cgggacggat tatagttatg gtagcttacc
    16201 ttatcgtatt ccgctaatgg ctggcagttc gcaacgtaca ttaccgacag tttctgaagg
    16261 agcattaggt tattgggctt taacaccaaa tattgatctg tggggaatgt ggcgttcacg
    16321 agtattttta tgaactgatt caacaaccgg tattcgtgat gaaggggtgt ataacagcca
    16381 gacgggaaaa tacgataaac atcgcgcacg ttctttttta gccgccagtt ggcatgatga
    16441 taccagtcgc tattctctgg gggcatcggt acagaaagat gtttccaatc agatacaaag
    16501 tattctcgag aaaagcatac cgctcgaccc gaattatacg ttgaaagggg agttgctcgg
    16561 cttttacgcg cagctcgaag gtttaagtcg taataccagc cagcccaatg aaacggcgtt
    16621 ggttagtgga caattgacct ggaatgcgcc gtggggaagc gtatttggca gtggtggtta
    16681 tttgcgccat gcaatgaatg gtgccgtggt ggataccgac attggctatc ccttttcatt
    16741 aagtcttgat cgtaaccgtg aaggaatgca gtcctggcaa ttgggcgtca actatcgttt
    16801 aacgccgcaa tttacgctga catttgcacc gattgtgact cgcggctatg aatccagtaa
    16861 acgagatgtg cggattgaag gcacgggtat cttaggtggt atgaactatc gggtcagcga
    16921 agggccgtta caagggatga atttctttct tgctgccgat aaagggcggg aaaagcgcga
    16981 tggcagtacg ctgggcgatc gcctgaatta ctgggatgtg aaaatgagta ttcagtatga
    17041 ctttatgctg aagtaaaaaa taacgccgga gagaaaaatc tccggcgttt tgtctgtgaa
    17101 ttactgctgc ggaatagcgg gtacgaccgg aacatttaat gccggcattg caaacatacc
    17161 aacgaaatct tccagtggca ttttctgccc gtttaacgtt atctgaccgt tagtatattg
    17221 caggctggtg gtgatggtat tgtcctgcaa ggtggtcaga cggaacatct gccccattgc
    17281 tgatgcacct tcaacttgct gtttcgccag ttttttcgct tgatcttcct gataaccttc
    17341 cagcttcgct acctgagtca taaactcagt tgccatatcc accggaatgg tcagtttcgc
    17401 atccagagat ttaaccgaac gatctacttc ctgcgccagc gtttgcggcg cttctttagt
    17461 cgttgccgga tctttcagga acagcgacag attcagcgca ctttcaccct gactgttttt
    17521 ccagcttagc ggcgcgatag taatcaccgg atcgcctttc aacatcagcg gcagggcgct
    17581 aaagaaggct tccgtcactt tctcctgata aagttcggga ttgttggcaa tttctggctg
    17641 tgccagcagc gcctgagttt gcgcgttata ttgctggcta aactgatgcc aggcttcacc
    17701 atcaatctgg ccgactttta aagtcagctt gccgctgccc agatcctgat tctgtacctt
    17761 caggctgttt agcgagtaat ccagttggct attgatcgtt ttaccgtcat tgaccagatc
    17821 cgatttaccg ctgatctcca tgccttccag cagtgccagt tctttgcctt ccactgaaat
    17881 ggtcattttt tccagtgaca gtttttggtt tcctacgcgc tcaccaaaac ttgccagcgt
    17941 gctggaaccg tcggttttca gattattaaa ggtcaactgc actttctggt tatattcgtt
    18001 aactgcgtct atccgtccac tttgcgcctc cccggaaagg gagatggctt tgccgtctct
    18061 gtcagcattt aactggaact cgccgccgct aaaggcgact ttttcatcct tttgctcgta
    18121 attcagtggc ttgagcgaaa tatcggaact ggaatcaccg ctgtaaccaa tgcgcgagtt
    18181 aatctcaaaa ggcgtttcac cttttgccat atcaaacagt ggtttgctta cttcgttatt
    18241 aaccagcgtg gtttgaattg atgccatcga cgggatcagg ttcagttttt taagctgggc
    18301 aagcgggaag ggaccatgat caaccgattc gttgaagatg acgctctgac cgcttttaat
    18361 ccacggattt tctttcccgg caatgggttt caccaacagt tgcaactggc tgctgaatac
    18421 gccgcgatga tagttttgat aactcacttc caggttggat tcaggagctg tcagtttgag
    18481 ttgcgcgttc gcctgcgcga ccatgtcttc gagatgggtt tcaatcttct tgcctgtata
    18541 ccatgcgccg cctgtccaga ctacgcctag cgcaacaatg acgcctaccg ctaccagcga
    18601 tttattcata atgattatcc ataaaatgaa atcaggcgga ctggccgcct gaaggtgtta
    18661 taagccttta ataagcttag caagagatgt taattttttc agtaagctct tacagcttgt
    18721 tgtaaacacg cgctaaacgg ccgtggcctt tgacagtcac cggtgattcg ttggcggcaa
    18781 taaacgctga ttcacccggt ttaagctgta actgctgaga acctttccac aacgttgcat
    18841 cgccttcgac gcagaacaaa atggcggcac tctgctggct aatggtggtt tctttatcac
    18901 taaggtcatg cagcgagaag gcaaaatcat ccactggaat cgggaagtcc agttctgcac
    18961 cttgtttcac cggctgggtc aacaactggt tagccggttt ggcttcgaat ttcacattgg
    19021 caaccagttc cggaatatca atgtacttag gcgtcagacc cgcacgcagc acgttatcgg
    19081 agttcgccat cacttccagc gccacgcctt gcaggtaagc gtgcggtgtt tcagcgaaca
    19141 ggaacatcgc ttcgccagga ttcaatttca ccacattcag taacagcggg gagaacaggc
    19201 cgctgtcttc cgggtaaaat tcagaaatta aacgaatcgt ttgccacggt tcaccctgct
    19261 gactgtctag cgccgatttt aaaatcgcca gcgcgcggga tttttcttca ccctgcatat
    19321 tcaacaggct ggcgaacagt tcgcttaaac gttcggcatt aggctgttgt aaaaagtgag
    19381 caattgccgg atgtgcacct gcgactggct ggagtaggga gacaatctcg gaaaactcac
    19441 gaaacgcgtt catcgcaagg aaaggcgtca gcgcaaaaac cagctccggc ttgtggttag
    19501 gatctttata gttacgctcg gcggcatcca tcgggatacc tgcggcattt tctttggcaa
    19561 aaccgatttc agaattgcgt ttgtttggat gaacctgaat ggagagcggc tgtgctgcgc
    19621 ataatacttt gaacaggaaa ggcagttcgc caaagcgttt ggcaacggcc tctccgagca
    19681 gagtcgattt atcactctca atcacatcac gcagtgaaac gatatctccg gcggcattct
    19741 gcactcgtga actgcttttc ggatgtgcgc ccatccacag ctcggccatc ggctggctgg
    19801 acggattttc cataccataa agttcagtca acgccgtttt gctgccccag gcatagtttt
    19861 gcactgagtt aatgagtttt tgcatgatca atccctgttt taatgtggaa attaatccca
    19921 ctattaaagc aagaatccta cgggaagtaa cctggagccg caaaaagtcg tactagtctc
    19981 agtttttgtt aaaaaagtgt gtaggatatt gttactcgct tttaacaggg caacggaaca
    20041 cccgcccaga gcataaccaa accaggcagt aagtgagaga acaatgtcaa acaaaccctt
    20101 tcattatcag gctccttttc cactcaaaaa agatgatact gagtattacc tgctaaccag
    20161 cgaacacgtt agcgtatctg aatttgaagg gcaggagatt ttgaaagtcg cactcgaagc
    20221 gttaactctg ttggcgcgtc aggcgtttca tgatgcgtcg tttatgctgc gtccggctca
    20281 ccaacaacag gtggccgaca ttctgcgtga cccggaggcc agcgaaaatg ataaatatgt
    20341 ggcgctgcaa ttcctgcgta actccgacat cgcggcgaaa ggcgttctgc caacctgtca
    20401 ggataccggc actgcgatta ttgttggtaa aaaagggcag cgtgtatgga ccggtggctg
    20461 tgatgaagcg gcgctggcgc gcggtgtcta taacacttat atcgaagata atttgcgcta
    20521 ctcgcaaaac gcgccgctgg atatgtataa agaggtgaat accggcacca atctgccagc
    20581 gcagatcgat ctttatgccg ttgatggcga cgagtacaaa ttcctctgta tcgccaaagg
    20641 tggtggttcg gcaaacaaga cgtatctcta tcaggaaacc aaagcgttac taacgccggg
    20701 gaaactgaaa aattacctgg ttgagaagat gcgcacgctg ggtacggcgg cctgtcctcc
    20761 gtatcatatt gcgttcgtaa ttggtggaac ttctgcagaa actaacctta aaacagtgaa
    20821 actggcttcc gctaaatact atgacgaact gccaacggaa gggaatgagc acggtcaggc
    20881 attccgcgat gtggaactgg aaaaagaatt gctgatcgaa gcgcaaaatc tcggtctggg
    20941 tgcgcagttt ggtggtaaat acttcgctca cgacatacgc gtgattcgcc tgccacgtca
    21001 cggcgcatcc tgtccggttg gtatgggtgt ttcctgctct gctgaccgta atatcaaagc
    21061 gaagatcaac cgtcagggga tctggatcga aaaactggaa cataatccag gtaaatatat
    21121 ccctgaagag ctgcgtaaag cgggagaagg cgaagcggtg cgcgttgacc ttaaccgtcc
    21181 gatgaaagag atcctcgcac agttgtcgca gtatcccgtt tctacacgct tatcgcttaa
    21241 cggcacgatt atcgtcggtc gtgatattgc tcacgccaaa ctgaaagagc ggatggataa
    21301 cggtgaaggg ctgccgcagt acattaaaga tcatccgatt tactacgcgg gtccggccaa
    21361 aacgccggaa ggttatgcct ccggttctct tggcccaacg accgccggac ggatggattc
    21421 ttatgtcgat caactgcaag cgcagggcgg aagtatgatc atgctggcga aaggcaaccg
    21481 cagccagcag gtgacggatg cctgtaaaaa acacggcggc ttctaccttg gcagtatcgg
    21541 tggtccggcc gctgtattgg cgcagggaag tattaagagc ctggaatgtg ttgaatatcc
    21601 ggaactggga atggaagcca tctggaaaat tgaagtggaa gatttcccgg cgtttatcct
    21661 tgtggatgat aaaggaaatg acttcttcca gcagatacaa ctcacacagt gcactcgctg
    21721 tgtgaaataa acagagccgc ccttcggggc ggttttttta catggcacga aagaccaaac
    21781 atttgttatc aaatagtaaa taataagtga gctaaaagtt gcttaacgaa agcaaaacag
    21841 aaagaaaaaa ttaaacaggt gaggagcagg ccatgaatac agtacgcagc gaaaaagatt
    21901 cgatgggggc gattgatgtc ccggcagata agctgtgggg cgcacaaact caacgctcgc
    21961 tggagcattt ccgcatttcg acggagaaaa tgcccacctc actgattcat gcgctggcgc
    22021 taaccaagcg cgcagcggca aaagttaatg aagatttagg cttgttgtct gaagagaaag
    22081 cgagcgccat tcggcaggcg gcggatgaag tactggcagg acagcatgac gacgaattcc
    22141 cgctggctat ctggcagacc ggctccggca cgcaaagtaa catgaacatg aacgaagtgc
    22201 tggctaaccg ggccagtgaa ttactcggcg gcgtgcgcgg gatggaacgt aaagttcacc
    22261 ctaacgacga cgtgaacaaa agccaaagtt ccaacgatgt ctttccgacg gcgatgcacg
    22321 ttgcggcgct gctggcgctg cgcaagcaac tcattccgca gcttaaaacc ctgacacaga
    22381 cactgagtga aaaatcgcgt gcatttgccg atatcgtcaa aatcggtcga acccacttgc
    22441 aggacgccac gccgctaaca ctagggcagg agatttccgg ctgggtagcg atgctcgagc
    22501 ataatctcaa acatatcgaa tacagcctgc ctcacgtagc ggaactggct cttggcggta
    22561 cagcggtggg tactggacta aatacccatc cggagtatgc gcgtcgcgta gcagatgaac
    22621 tggcagtcat tacctgtgca ccgtttgtta ccgcgccgaa caaatttgaa gcgcttgcga
    22681 cctgtgatgc cctggttcag gcgcacggcg cgttgaaagg gttggctgcg tcactgatga
    22741 aaatcgccaa tgatgtccgc tggctggcct ctggcccgcg ctgcggaatt ggtgaaatct
    22801 caatcccgga aaatgagccg ggcagctcaa tcatgccggg gaaagtgaac ccaacccagt
    22861 gtgaagcatt aaccatgctc tgctgtcagg taatggggaa cgacgtggcg atcaacatgg
    22921 gtggcgcttc cggtaacttt gaactgaacg tcttccgtcc gatggtgatc cataatttcc
    22981 tgcaatcggt gcgcttgctg gcagatggca tggaaagttt taacaaacac tgcgcagtgg
    23041 gtattgaacc gaatcgtgag cgaatcaatc aattactcaa tgaatcgctg atgctggtga
    23101 ctgcgcttaa cacccacatt ggttatgaca aagccgcgga gatcgccaaa aaagcgcata
    23161 aagaagggct gaccttaaaa gctgcggccc ttgcgctggg gtatcttagc gaagccgagt
    23221 ttgacagctg ggtacggcca gaacagatgg tcggcagtat gaaagccggg cgttaatctg
    23281 caacatacag gtgcagccgt ggaatgatca aacgaagcgg ctgcgcctgt ggtttataac
    23341 ggtgctgcac attgtcggca tcgtaattta acaactcacc aacgtctggc acgcccgcac
    23401 cattatcccg attaatcagt gcaatcagcg gtgtagggca ggcgtgttgc acttgttttt
    23461 gatctccttt gtaccagacg cgggcaatcg gctgcacctt caccggacgt ttgattttta
    23521 acttcgcgtt ctgtggcagg gcagcgatat cctgatactc tcgctccagt tttctttgcc
    23581 actcctcgcg cgtccacggt gcgacactgc gtggtgattt caggcttttt tccagctgtg
    23641 ccaggacttc atcacgatgc aaattcttaa tgatatgttt attagcccaa ccaaagcgta
    23701 aagtggcggg gtcgtgcaga acggtgagcg tgcggtaagc attaagggtg atcagccccg
    23761 gcaaatgacg atgcacccat tcaaaacgtg ccgcggtggg gagttctgat tcaaccgtga
    23821 cgatatgctc gaacgtggtc ttgagtttat tgatgtgctg aatatgactg accaacgctg
    23881 cttgcgaaag gttatcgacc tggtaacaca acacgccagg cagacggaca gcggccttgc
    23941 tgctgcgatt ttcggactgt tgttgaataa ataaatggcg gaaatgacgc aacgccagcg
    24001 actgcgcgtc gttgccgaga tgttgtttta cctcaatacg attaagcgga ttatgctcat
    24061 cctctttttt tacctccggc aaagagaaca cgcgggcaac caatagcttg tgttgctcaa
    24121 gatgagcggc aaatgtagcc agctcttgtt ccatctggcg aaaggtagtg ttgagtcggt
    24181 ctacgagatc gtaacgcgcc ataatattaa ccactttagt tacaacatac ttattttata
    24241 gcacagtcgt ggtgacttga ccatcgtgcg cgtagtgtca ggcagaggta aattgcggga
    24301 tgttatgcca taacggccag ctaaacgaga agcgagcacc acccagttcg ctggtgtcac
    24361 aattaaccgt accgcccatt gccagtgcta tagagtggac aattgccagc cccagcccgc
    24421 agccgccggt tgagcgatcc cggctgggat cgaggcgaac aaaaggttca aagatatgtt
    24481 cgcggttttc tggggcaatc cctgggccat catcctcaac aattaatgtc gctctattcc
    24541 ccgacagtag caggctggtt tcaaccgttg aatggcagta ccgcagggcg ttattgagca
    24601 aattatccag cacgcgctcc attaagcgca tatccaacgc cgcataatgg ccttgcacga
    24661 gcgttttaat ccgtaccgtt ttatcgggcg ttactgcctg aatgtctgcc agatgcgttg
    24721 acagccacaa cggcaggtct ggttcgctaa gatgaagctc gttttgtggg cgatcgagtc
    24781 gggcataagt cagcagctct tcaattaaag cttcaagttg actgatatca cgattcaacg
    24841 cctgggattc ggcggcgctc aggttatcgc tcatctccag tcgataacgc aggcgcacta
    24901 acggtgttcg cagttcgtga gcgataccgt caataagctg ttttttgctg gcaatcaagg
    24961 cgttgatatt gtccgccatc tggttaaatg cgataccaag tcgttcaaag ctcgaaccct
    25021 catcaaagtg gatacgctca ttgagatgcc catcgccaaa tcgttgcgcc gctgcttcca
    25081 gttttaacat atcctgccag tgcggacgca tccagataaa caccggaaag gcgagggaaa
    25141 tagcaataaa agcgatcagg gcgatatcca gcaatcgcat ctgatggaga taataaagat
    25201 aaggaacagg accaactgcc agcacgtagt ggctgcgcgg gatacgctgc aaaaacgtgt
    25261 actgatcgtc cagggcgaca atttcgccgc cacgcagtcg gtgcatggaa atatcatcaa
    25321 gatggtattt actcaatggc tcgacacgca gatcgaaaga gagatttaaa tccatctctt
    25381 tcagcgtctt accccagtcg tgtgggggga tctcacgcaa ttcgctgcgc atcagataca
    25441 gcgaactgtt catcaaatca tccagcgact gtttgcccgc gcgttcggcg gtaaatttgt
    25501 acaccaaccc aaccagcaga gacatcacaa ggaagcagac aaacaataac aggtaaaact
    25561 ggataaacag ttttttcatc gcttattccc atgcatgagg cgcaaaaaga tagcctttgt
    25621 tacgcacagt tttaatgcga taaggttctg cggcgttatc gagcagtttt tttcttaacc
    25681 gcgaaatagc cacgtccacg ctacgatcca gtccgtcata actgacgccg cgtaaatttt
    25741 tcagcaatgc atcgcggtcc atgatttgcc cggcatgggt agctaattcc cacaataatt
    25801 cgaaatcagc tgtcgagagc gagatttcag tgttagccag ggtgactacg cggttgatgg
    25861 gatcgatggt caacgtgccg aaatgcaggg ctttgtaggg agtcagagac gtttcctgaa
    25921 gacctttggt cagtgtggct tgctcattct gacgcaaatg caaacgtaaa cgcgctagca
    25981 aaacagcagg gggcgtcgtt ttgagaatat agtcgcaggc acccatttcc agtgccagga
    26041 tgtggttcat atcgctatcg agagaggtta gaagaacaat cggtccagac cactttgcgc
    26101 gtaaatcacg acaaatggtc atgccatcct tgcctggtag catgatgtcg agtaacacca
    26161 aatccggact ttctcgcaaa atggtttctt cggcctggtc gccgcgcggt tctacggtaa
    26221 cctgcatatc atgttttgcc aggtacgcgg caatcagtga accgacttcc gcatcatctt
    26281 ccacaaatac gatagtgttc ataacattca cggtagatat aaaaacgtca caatacaccg
    26341 cgctgttttt acttactatc aatcgtttct aaacaagttt tcatcaggta ttctactgat
    26401 gacttgttat ggaagtgtta aggtaaaaag atggggctgg taatcaaagc cgcgctcggt
    26461 gcgctggtag tgctgttgat tggtgtttta gcaaaaacga aaaattatta tatcgccggg
    26521 ctgattccac ttttcccgac ctttgcgctt atcgcgcatt atattgttgc cagcgaacgc
    26581 ggcattgaag ccttacgcgc aaccatcatt tttagtatgt ggtcgattat tccctatttt
    26641 gtttacctgg tgtcgctgtg gtatttcacc ggaatgatgc gcttgcccgc cgcttttgtt
    26701 ggttctgttg cgtgttgggg gataagcgca tgggtgttga ttatatgctg gataaagctg
    26761 cattaacgca gatgacgacc gccatcaagt gggaaactgc gtccggtgac aaagcagctg
    26821 gtaagtaagt aatcgaccag gtcgatcact tctttctcgc caggcgcggt tttcatcagt
    26881 gatttattca gcgcctgttg tcgatattcg gcatcatcat gttcattaaa caggatcagc
    26941 gatggcgcaa tagaattcac tttcacttcc ggtgccagct tgcgggcaaa cgagcgggtc
    27001 atattatcca gtgccgcttt gcttgcagca tacgcaacat gtttgtcgct accgcgctcc
    27061 accacataat cggtaaagtg aatgatatcg ctggcggcgt gtccgtgccc acgcagtaat
    27121 ctttccagcg catggttgag caggtatggg gtattaacgt ggatctgcat catgcaagcc
    27181 agtacgtcgg ccagtggtgt acctggtttt tccgccatcc acgcactggc gttatgcaaa
    27241 atagcacgca gaccatgggt gctttttagt acttcatcgg caaacgccat cacaccgtcg
    27301 ttggtcgaaa aatcagcctg aatacactgc gcacctgcct taatcagtcc atcaatggct
    27361 ggatagtgcg tccgatagct gacaatcacc ggttgctttt gattaatgaa atgccaggcg
    27421 agggcgaggc cgatgcgacg acctccgcca gtaattaata ttggcaaggg ctgggcttta
    27481 cccatcgtta tctcctttgc tatccaacga tgggatgaac gttatcccac cagcatccag
    27541 gtcgccggaa ctgaggcaat taacagcatg ccaatcagca ccatttcctg acggttcagt
    27601 acattatcat gtgtatgcgt tttgcgcgca tacaggaaaa ccagcagccc cggcgcatac
    27661 agaacaacgg ataacagcaa gtgcattgga ccagaggcat acaataacca taagccataa
    27721 atgcaggcac cgacacctac cgctttatgc agtggacggg ttgcgatttt cagcaagaac
    27781 gcgccgacca ggaaataggg caccagaatc atttctgagg cgatggtcag caacgtgttg
    27841 taatcggaac cggtcagcca gatgagtacc agacaaattt gcacgcagat gttggtcaac
    27901 cacagcgagg cggatggcgc agcttgtgcg ttctggcgcg caaaaatgcg tgggaatgct
    27961 ttatgagtgg cagccaggaa cggaacttct gccgccatga tggtccagct taagtacgcg
    28021 ccgcaaacgg aaacgatcaa accggcagcg atgatgattt cgccccatgg tcccatcatt
    28081 tccaccatca gaccggccat tgacgggtta cgaatttcag ccagttcagg acgtgccacc
    28141 acgcccagcg aaagcagcgt taccagcaag taaacgccca gagcggagag aaccgccagc
    28201 agtgtcgctt tgccaacatc acgtttatta cgcgcacgcg cagaaacaac cacggcacct
    28261 tccacaccaa tgaaaaccca cagggtgatc agcatggtgt ttttcacctg ttcccacacg
    28321 ggtacgccaa gtgcaagtcc ggtgaagtcg agcttgaaag tatccagttt gaacatcatc
    28381 atcgccagca caacaaacag acccagcggc aacaattttg ccagtgtcgc caccaggtta
    28441 atgctggcag cggtttgcac cccgcgcagg atcaaaaagt gaacaatcca caataaagcc
    28501 gatgcaccga ctatcgattg ccaggtatta ccatcgccga acaggcgcaa ttccggcgtg
    28561 tccgtaaaaa agcttaacgc ggaaaaaacg atcaccagat aggagacgtt ggcgatgact
    28621 gcgcacagcc agtatcccca tgcggaacaa aagccgatta gctcgccaaa cccttcgcgg
    28681 gcataggtaa agataccgcc gtcaagttcg ggacgaatgc gcgtgaggat cagcatggca
    28741 aaggccagca ataaaatgcc agcgccagta ataccccagc cgatgagcag tgctgccggg
    28801 ctggcaactg ccgccatatt ttgcggcaga ctgaaaacac ccgcgcccag cattgagctt
    28861 aataccagcg cggtgagtgc gctcagtccc agtttctttt ccatcgatat ccagtgatta
    28921 aaacgtgata agaagaataa atattagggc aaaacgcata aaaatggagt ttgccactga
    28981 agcgcgggat tttacgaaga gatgtgtctg catgcaatgg tcgcggggaa atttgtgact
    29041 aaaaatgcga aaggcggcat caagccgcct tataggagta actgaatagt tatttataca
    29101 gatctgcgct gacggtcagg ttattaccac gttcctgcca ctggcgggtg atgtggtaat
    29161 acttagcacc tttcttcgcg gcacgtttcg caacctgata ggagacttcg gtcatgttgc
    29221 cgtagttgcc agagaatttg atgctgtcga acgggaccat catcgctgct gtcgctttgt
    29281 tcagttcttc gactttagtg ccgtcaggaa gcgtgacagt gtaacgtccg ccttttgatg
    29341 actgggtttc aaagaaacga ccgacttcag aacttggtga tgcggtagtc gcaacacctg
    29401 ggatctcaac tttcttcgcg gcttcgccac cggcagccag agctgcacgt cctgcttcgg
    29461 aatctgccgg gatcacatcc gggctctgga cgatacgttt cttagcatct tttttataga
    29521 tgaatgcagt aatacgctgg ttgccgccct ggttggcatc gatttgacga acaatgtaga
    29581 aagagtaggc acctttcgct tttgctgctt tggtgatggc atcattgact tctggctggc
    29641 tacgatagaa gccctggacg gtgaccgtgt caaacggttc aatcagaaca gcctgatctt
    29701 tcggcagttc gacaacaccg ttaattacgc gattacttgt ttcttctgct ttttcagcat
    29761 cggctttata gaggtcagcg accacacgcc agttaccgct gttaccaaaa tcagaagtgt
    29821 cgacaacata aaaagaggcg gcaccttctt tatctgcgcg acgagaaacg gctttcaccg
    29881 cttcgccaat agcattaaaa cgaccggtaa ccactacacg gtcaaaaggt ttaaccgctg
    29941 ccgcttgctc cggtgtcagt tctgttgctg cgttaacgga gaatgccgta gcagaaagca
    30001 gtgccgacgc caggagggtg ttcttaagct tcataaaaat aatccttcgc cttgcgcaaa
    30061 ccaggtactg gtattgttat taacgagaaa cgtggctgat tattgcattt aaacggtgta
    30121 actgtctgcg tcatttttca tatcacattc cttaagccaa ttttaatcct gctcaaatga
    30181 ccgtctatgc ttaaaaaaca gccgtatcag catcattact actgaagcaa ctgaattgta
    30241 taagttaatt taatgttaag tagtgattcg tgccggggcg atgtctcgtt ttacccgacc
    30301 gtcgaagaca attatcagtc tttatccggc gttctaaggt gtttatccca ctatcacggc
    30361 tgaatcgtta atattttgcg agttcacgcc gaaatactga tttttggcgc tagatcacag
    30421 gcataatttt cagtacgtta tagggcgttt gttactaatt tattttaacg gagtaacatt
    30481 tagctcgtac atgagcggct tgtgtggctc ctgacacagg caaaccatca tcaataaaac
    30541 cgatggaagg gaatatcatg cgaattggca taccaagaga acggttaacc aatgaaaccc
    30601 gtgttgcagc aacgccaaaa acagtggaac agctgctgaa actgggtttt accgtcgcgg
    30661 tagagagcgg cgcgggtcaa ctggcaagtt ttgacgataa agcgtttgtg caagcgggcg
    30721 ctgaaattgt agaagggaat agcgtctggc agtcagagat cattctgaag gtcaatgcgc
    30781 cgttagatga tgaaattgcg ttactgaatc ctgggacaac gctggtgagt tttatctggc
    30841 ctgcgcagaa tccggaatta atgcaaaaac ttgcggaacg taacgtgacc gtgatggcga
    30901 tggattctgt gccgcgtatc tcacgcgcac aatcgctgga cgcactaagc tcgatggcga
    30961 acatcgccgg ttatcgcgcc attgttgaag cggcacatga atttgggcgc ttctttaccg
    31021 ggcaaattac tgcggccggg aaagtgccac cggcaaaagt gatggtgatt ggtgcgggtg
    31081 ttgcaggtct ggccgccatt ggcgcagcaa acagtctcgg cgcgattgtg cgtgcattcg
    31141 acacccgccc ggaagtgaaa gaacaagttc aaagtatggg cgcggaattc ctcgagctgg
    31201 attttaaaga ggaagcgggc agcggcgatg gctatgccaa agtgatgtcg gacgcgttca
    31261 tcaaagcgga aatggaactc tttgccgccc aggcaaaaga ggtcgatatc attgtcacca
    31321 ccgcgcttat tccaggcaaa ccagcgccga agctaattac ccgtgaaatg gttgactcca
    31381 tgaaggcggg cagtgtgatt gtcgacctgg cagcccaaaa cggtggcaac tgtgaataca
    31441 ccgtgccggg tgaaatcttc actacggaaa atggtgtcaa agtgattggt tataccgatc
    31501 ttccgggccg tctgccgacg caatcctcac agctttacgg tactaacctc gttaatctgc
    31561 tgaaactgtt gtgcaaagag aaagacggca atatcactgt tgattttgat gatgtggtga
    31621 ttcgcggcgt gaccgtgatc cgtgcgggcg aaattacctg gccggcaccg ccgattcagg
    31681 tatcagctca gccgcaggcg gcacaaaaag cggcaccgga agtgaaaact gaggaaaaat
    31741 gtgcctgctc accgtggcgt aaatacgcat tgatggcgct ggcaatcatc cttttcggtt
    31801 ggctggcaag cgttgcgccg aaagagtttc ttgggcactt caccgttttc gcgctggcct
    31861 gcgttgtcgg ttattacgtg gtgtggaatg tatcgcatgc gctgcataca ccgttgatgt
    31921 cggtcaccaa cgcgatttca gggattattg ttgtcggagc actgttgcag attggccagg
    31981 gcggctgggt tagcttcctt agttttatcg cggtgcttat agccagcatt aatattttcg
    32041 gtggcttcac cgtgactcag cgcatgctga aaatgttccg caaaaattaa ggggtaacat
    32101 atgtctggag gattagttac agctgcatac attgttgccg cgatcctgtt tatcttcagt
    32161 ctggccggtc tttcgaaaca tgaaacgtct cgccagggta acaacttcgg tatcgccggg
    32221 atggcgattg cgttaatcgc aaccattttt ggaccggata cgggtaatgt tggctggatc
    32281 ttgctggcga tggtcattgg tggggcaatt ggtatccgtc tggcgaagaa agttgaaatg
    32341 accgaaatgc cagaactggt ggcgatcctg catagcttcg tgggtctggc ggcagtgctg
    32401 gttggcttta acagttatct gcatcatgac gcgggaatgg caccgattct ggtcaatatt
    32461 cacctgacgg aagtgttcct cggtatcttc atcggggcgg taacgttcac gggttcggtg
    32521 gtggcgttcg gcaaactgtg tggcaagatt tcgtctaaac cgttgatgct gccaaaccgt
    32581 cacaaaatga acctggcggc tctggtcgtt tccttcctgc tgctgattgt atttgttcgc
    32641 acggatagcg tcggcctgca agtgctggca ttgctgataa tgaccgccat tgcgctggta
    32701 ttcggctggc atttagtcgc ctccatcggt ggtgcagata tgccagtggt ggtgtcgatg
    32761 ctgaactcgt actccggctg ggcggctgcg gctgcgggct ttatgctcag caacgacctg
    32821 ctgattgtga ccggtgcgct ggtcggttct tcgggcgcta tcctttctta cattatgtgt
    32881 aaggcgatga accgttcctt tatcagcgtt attgcgggtg gtttcggcac cgacggctct
    32941 tctactggcg atgatcagga agtgggcgag caccgcgaaa tcaccgcaga agagacagcg
    33001 gaactgctga aaaactccca ttcagtgatc attactccgg ggtacggcat ggcagtcgcg
    33061 caggcgcaat atcctgtcgc tgaaattacc gagaaactgc gcgctcgtgg tatcaacgtg
    33121 cgtttcggta tccacccggt tgcggggcgt ttgcctggac atatgaacgt attgctggct
    33181 gaagcaaaag taccgtatga catcgtgctg gaaatggacg agatcaacga tgactttgct
    33241 gataccgata ccgtactggt gattggtgct aacgatacgg ttaacccggc ggcgcaggat
    33301 gatccgaaga gtcctattgc tggtatgcct gtgctggaag tgtggaaagc gcagaacgtg
    33361 attgtcttta aacgttcgat gaacactggc tatgctggtg tgcaaaaccc gctgttcttc
    33421 aaggaaaaca cccaaatgct gtttggtgac gccaaagcca gcgtggatgc aatcctgaaa
    33481 gctctgtaac cctgacggcc tctgcggagg ccgtcactct ttattgagat cgcttaacag
    33541 aacggcgatg ctttgacctc ccgctgtttg ttcaagcgca attttgacaa taattgttaa
    33601 cggcacggaa agcagcatac ccaccggtcc taacaaccat ccccaaaaaa tcaacgacaa
    33661 aaataccacc aatgtggaaa gccccagccc acgtcccatg atgcgcggct ccagaatatt
    33721 gccgaagacc agattaatca gcagatatcc cgccagcacc agcaacgctt cgtagaagcc
    33781 attaaacacc agtacctgag cgataggggg gattgccgcg agaactgaac caatattagg
    33841 gatgtaatta agcgcaaagg ccagcaatcc ccagacaaaa gcgaagcgaa catcgagtgc
    33901 ggcgagcatc gcccaggcga ccaggccggt gatgatgctg atggctgttt tcagcaccag
    33961 ataatgagaa acactgtcaa ttgcacgttg aatcgccgcc atcccttcaa ccggacgcgc
    34021 catcatttgc tgaaattttc cgggcaattg tggcacttcg agcagcataa acagcaccgt
    34081 cagcagcaat aaaaatattg atgacatggc attagataac tgcgtcaata agttggtgag
    34141 caacgtcatc gccgcgttcg gatcaatgta atgcgccagc tggtcaactg agacgtcaat
    34201 ccctacgcgt tgcaacaacg gctcaagagc ttgcagcggc gtcataatag agttgcgata
    34261 ttgcggtaac gtccgcgtca actcgttgag cgcggaaccc agataagcta atagcaacac
    34321 catcgccatc acgatgatgg tcatcaaaat cgacaccgcc agtacacgcg gcactcgcca
    34381 gcggaccatg tgttgcacca gcgggttaag aataacagca ataaataatg cgagaataaa
    34441 cggcacgatg atctcggcgg caaaacggat accgcagaga ataatgacca gcattcccaa
    34501 cataatgacg atttttaggc cattgagcgt gatgatcggc tttgccatgc ttgtccccat
    34561 tttttcttcc tttgctaata ataatgtctt tttcgctccg tcatcataac gaaaatcaaa
    34621 ccgctcaggt aaagaagtga aaatattttg agttaattct taagctatga tacaaatcag
    34681 gcgtgttcaa ctaccgagga caattatcat ccgcgatgac gagaagcaac actgcggata
    34741 attgtaatat tatggacaat ttgttcagga cgttattttc aacatttacc catcttcgta
    34801 cctcatccac tatcttgctc gtaggtgagc agcactggcg caacgcgctt tagtcctttc
    34861 ttctgcctaa gcttgcccta tgcgcggggc ttgaccactt tactttggtt tcgctgaatt
    34921 aagcgaaaat taaaataatt ctcttgcagg agaaggacaa tgtatattta ttggatttta
    34981 ttaggcctgg ctattgctac agaaattacc ggtacgctgt caatgaaatg ggcgagcgtc
    35041 agtgagggaa atggcggctt tattttaatg ctggtgatga tttctctgtc gtatatattt
    35101 ctctctttcg ccgttaaaaa gatcgcctta ggcgtagctt atgcgctgtg ggaaggtatc
    35161 ggtattttat ttattacctt gtttagcgtt ttgttattcg acgaaagttt atcgctgatg
    35221 aaaattgccg ggttaaccac cctggtcgcc gggattgtgt tgatcaaatc aggtacccgt
    35281 aaagcgcgta aacctgaact ggaggtgaac catggcgcag tttgaatggg ttcacgccgc
    35341 ctggctggca ttggcaatcg tgctggaaat cgttgctaac gtctttttga aattttctga
    35401 cggctttcgt cgcaaaatat ttggcttgct ctccctggcg gcggtgctgg ctgcctttag
    35461 tgcgctttct caggccgtaa aagggatcga cttgtctgtc gcttatgcat tgtggggcgg
    35521 gtttggtatt gccgccacgt tagccgcagg ttggatcttg tttggtcaac ggttaaatcg
    35581 taaaggctgg attggcctgg tcttgctgtt ggctggaatg atcatggtga aacttgcctg
    35641 atgaacacgc tgcccgcact gtcgggcagc gtttgaacat tatttttgcg acagttgatc
    35701 cagcttgtcg cgaaaaccgg taacagaaat ggcccggtta tcggcgcgcc agcgatcttt
    35761 cgcggcagga gccgaacttt gcaccccaat taattgccag ccgtcatcgg tatgcaacat
    35821 cagaggcgaa ccgctgtcac ccggcaaggt atcgcactga tgtgacatca ctgacgtttg
    35881 cgcccagcca gtcacttcac agttttgatg actgtataac gtatcgagat gatcttcagg
    35941 atatcccgcc tgagtcactt tacgaccagc cgattttaat gcggcagtaa gcgcggcttt
    36001 atctccctca aataacggca acggtgtaat gccagaaggg ggattacgca gcacaatcaa
    36061 gccgaagtcc catggtgcag ctgcgggagg aacaatccaa ccatccccat ctgcttttaa
    36121 ccgctttccc agtgtcggat caacgcggcc ttctatgtcg tggatgtcat agcgccaaag
    36181 acctttattt gacacaaaac gcagcgctac cgccttatcc gctttaccct taggcggcgt
    36241 caataaacag tgtcctgccg ttaatgccag attgggtgca atcagcgtcg ccgtacataa
    36301 attgccgctg gccgtttcca gttgcccaac cgcatcccac ggtgattggg tcgtgtcatt
    36361 cactggcaca cgatcatcat gaccaaaaaa cagggtgctg acctcatcgt ttgccgattt
    36421 ggcaacgtct ggtttatctg caaacacaaa agcagacgtc aaactaattg cacccaacac
    36481 tacagcaatg gttgtacgca tatcacactc tggtgggtaa ttatgattat taaaagcaaa
    36541 ccctcataaa tactatagac gggacgggat gaaagtggga gtaaaatcag ataactacag
    36601 tcaggaaaga taaaaacggg cggcaatcag tgcgcataaa ataaggatga taagaatgaa
    36661 ctcaaagcga taacggccca ccatactacc ctccgctaaa ggcggtgcta aacgttaccc
    36721 gcttagcacc gtaattttca ggcgcgaggg ggcgcgccag cattactgtt gaaaacttac
    36781 gctgcgggtt gtgcagcagg ttttgccggt tgctgatggc tgtgtttccc ggcgtgtttc
    36841 ttcgctgcct gcgctttttg ttcaggggct ttctgttcag ctttcgtatt tttatgatgc
    36901 tttttagccg cctgcgcttt ctgggcaggt gctgctttat gctgtttttt atgatgtgta
    36961 gttttcgccg gcgctgcttt ggtggtcgtc gcagtcggag caggtgtggt cgcagtctct
    37021 gcagcaaagg cggcagaaga cagacccata gcagcggcaa caaccagacc taatactttt
    37081 ttcattgtca taccctcaat ttgttttttc atttaacccc actgcggggc cgttgaaata
    37141 actatatccc tgggaatggc agacttccgt gagtggttgg tttcagcgtg taacgatatg
    37201 tacaaacgct gaataaaata cagcattgat aataacgtat tgtgacttaa gggaaattta
    37261 gctaccaata atagtagtct tgatcgggtt aactatttta ctacttacaa agcggtagaa
    37321 taactgcgca tcagtaataa atgacacaca gcaaaatgaa tccgtttatt tgggtactta
    37381 taatcctgat gacgctagac gcgctgcggg aattggctgg cgcttcgtct attttaggat
    37441 ggctattaac gttggtttga gcaagcaata agtccggacg tgtagttacc gcagtccggg
    37501 cttatttttc aggcgtgcag acgacgatgc aaacgcgtcc cgaccagcag agcaatgacc
    37561 agcatcagag caataaatgc gccgacgccg ttccagccat agttatgcca gaaaacacca
    37621 cccagcgtcc cggcaatact cgaccccaga tagtaactga acagatacag cgaggaggcc
    37681 tggcctttag cgcgttttgc gcgggggcca atccagctgc tggcaactga gtgggctgcg
    37741 aagaatcctg ctgagaagag taacattccg gcaaagatca gccacagcga gctgaataag
    37801 gtcatcagta aaccaaacag cataaccccc gtcgaaaaca acatcaccgg accgcgcccg
    37861 tagcgggtgg tcatggttcc ggctttaggt gagctccatg taccggtcaa ataagccagc
    37921 gataataagc caaccacggc ctgactgaca tgccaggggg aaagcatcaa ccgatagccg
    37981 atgtaattaa acagcgtgac gaacgacccc atcagcaaaa agccttctgc gaacaataac
    38041 ggtaatcccc ggtcacgcca gtgcagacga aagttgataa acaacgtctt aggtcgcagc
    38101 gaagtcgggc gaaaatggcg tgattcaggg aggattttcc agaacatcaa cgccgaggct
    38161 agcgcgaaac aaccgattgc cgccagagca attcgccagt tgaaaaagtc cgtgaagaca
    38221 ccactaatta agcgcccgct catgccgcca attgagttgc cgctgatata caaccccatc
    38281 gaaaaggcca cgaaactggg gtggatttcc tcgctaagat aagtcatgcc aacagctgcc
    38341 acgccactta acgaaagccc aatcaaggcg cgcataatca aaatgccgtg ccagctggtc
    38401 atcattgtcg aaagtaacgt acaaatggag gccaacagta gcgccgtgac catcactggt
    38461 ttgcgaccaa tggcatcgga tagggggcca gtaaacagca aaccaatagc caacatcgcc
    38521 gtggaaatgg acagtgaaat actactgttc gcgggcgtta agccaaactc ctgcgaaagc
    38581 accggaagga taggctgcac acaatagaga agtgcaaatg ttgccagtcc ggcagagaac
    38641 agcgccaggg tgacgcgcat aaattgcggc gtaccgcgtt taataaattg atttggctga
    38701 gaaatgcttt gcttgtcagt gtcgcttgcc ggagcgccat caacagttgt agtacggctc
    38761 acttgaaatc cttgctaaat atgcctgtgg atcaggctta tacatagggt aggaaaatcg
    38821 aattgttctg tctaatatat taataatctc aaataagatg ttttaaatat gaatattgaa
    38881 cttcgtcatc tgcgttactt tgttgctgtt gcggaagagc tgcattttgg gcgcgcggct
    38941 gcccgcctga atatttcgca accgccgcta agtcagcaga ttcaggcact ggagcaacaa
    39001 atcggtgccc gactgctggc acgaactaat cgcagtgtat tgctgacggc agcaggaaaa
    39061 cagtttcttg cagatagtcg gcaaatcctg tctatggtgg atgacgctgc cgctcgcgct
    39121 gaaagactgc atcagggtga agcgggggag ttgcgtattg gttttacttc gtccgctcct
    39181 tttattcggg cggtgtctga tacgttatcg ctgtttcgcc gtgattatcc cgatgtccat
    39241 ttacaaaccc gcgaaatgaa cactcgtgag caaatcgctc cgctcattga aggaacgctg
    39301 gatatgggat tgctgcgtaa cacagcgtta ccggagacgc ttgaacacgc agtcatcgtc
    39361 catgaaccgc ttatggcgat gatcccgcac gatcatcccc tggcaaataa cccgaatgta
    39421 acgctggctg aactggcgaa agaacccttc gtcttttttg atccgcacgt cgggacaggg
    39481 ctgtatgacg atattctcgg gctgatgcga cgttaccatt tgacgcccgt catcactcag
    39541 gaggtgggcg aggcaatgac catcatcggt ctggtttccg ccggtctggg tgtttcaatt
    39601 ttgcctgcgt catttaaacg tgttcagctc aacgaaatgc gctgggtgcc gattgcagaa
    39661 gaggatgcgg tttctgaaat gtggttggtc tggccgaaac atcatgaaca aagtccagct
    39721 gcgcgtaact ttcgtattca tctgctgaat gctctcaggt gagggaaatt tcagcgaaaa
    39781 agcccgaaaa atgtgctgtt aatcacatgc ctaagtaaaa atttgacgac acgtattgaa
    39841 gtgcttcacc atagcctaca gattatttcg gagcgcgaaa atatagggag tatgcggtgg
    39901 ttgctgaaaa ccagcctggg cacattgatc aaataaagca gaccaacgcg ggcgcggttt
    39961 atcgcctgat tgatcagctt ggtccagtct cgcgtatcga tctttcccgt ctggcgcaac
    40021 tggctcctgc cagtatcact aaaattgtcc gtgagatgct cgaagcacac ctggtgcaag
    40081 agctggaaat caaagaagcg gggaaccgtg gccgtccggc ggtggggctg gtggttgaaa
    40141 ctgaagcctg gcactatctt tctctgcgca ttagtcgcgg ggagattttc cttgctctgc
    40201 gcgatctgag cagcaaactg gtggtggaag agtcgcagga actggcgtta aaagatgact
    40261 cgccattgct ggatcgtatc atttcccata tcgatcagtt ttttatccgc caccagaaaa
    40321 aacttgagcg tctaacttcg attgccataa ccctgccggg aattattgat acggaaaatg
    40381 gcattgtaca tcgcatgccg ttctacgagg atgtaaaaga gatgccgctc ggcgaggctc
    40441 tggagcagca taccggcgtt ccggtttata ttcagcatga tatcagcgca tggacgatgg
    40501 cagaggcctt gtttggtgcc tcacgcgggg cgcgcgatgt gattcaggtg gttatcgatc
    40561 acaacgtggg ggcgggcgtc attaccgatg gtcatctgct acacgcaggc agcagtagcc
    40621 tcgtggaaat aggccacaca caggtcgacc cgtatgggaa acgttgttat tgcgggaatc
    40681 acggctgcct cgaaaccatc gccagtgtgg acagtatact tgagctggca cagctgcgtc
    40741 tcaatcaatc catgagctcg atgttacatg ggcaaccgtt aaccgtggac tcattgtgtc
    40801 aggcggcatt gcgcggcgat ctgctggcaa aagacatcat taccggggtg ggcgcgcatg
    40861 tggggcgcat tcttgccatc atggtgaatt tatttaaccc acaaaaaata ctgattggct
    40921 caccgttaag taaagcggca gatatcctct tcccggtcat ctcagacagc atccgtcagc
    40981 aggcccttcc tgcgtatagt cagcacatta gcgttgagag tactcagttt tctaaccagg
    41041 gcacgatggc aggcgctgcg ctggtaaaag acgcgatgta taacggttct ttgttgattc
    41101 gtctgttgca gggttaacat tttttaactg ttctaccaaa atttgcgcta tctcaatttg
    41161 ggccaggaaa gcataactta gactttcaag gttaattatt ttcctggttt atatttgtga
    41221 agcataacgg tggagttagt gatgctgaag cgtttcttta ttaccggtac agacacttct
    41281 gtagggaaaa cggtggtttc ccgcgcattg ctacaagcgt tagcctccca gggaaaaacg
    41341 gttgcgggat ataaacccgt agcgaagggg agcaaagaga cacccgaagg gctgcgtaat
    41401 aaagatgccc tggtgttgca gagtgtttca accatcgaac tgccttatga agcagttaat
    41461 cctatcgcgt taagcgaaga agaaagtagc gtggcgcaca gttgcccaat caattacacc
    41521 ctcatttcaa acggcctggc aaacctgacc gaaaaagtcg atcatgtcgt ggtagaaggg
    41581 actggcggct ggcgcagtct gatgaatgat ttgcgtccac tctctgaatg ggtggtgcag
    41641 gaacaactgc cggtgttgat ggttgtcggt attcaggaag gttgcattaa ccatgcactg
    41701 ctaacagctc aggcgattgc caacgacggg ctgccgctca ttggctgggt ggctaaccga
    41761 atcaacccag gactggcgca ttatgcggaa atcattgatg tgctcggtaa aaaacttccg
    41821 gcaccgctca ttggtgaact gccttatctg ccgcgcgccg aacagcgtga actggggcaa
    41881 tacatccgct tagctatgct gcgcagtgtg ctggcggtag atagagtcac ggtgtaacgt
    41941 ccgcgagatt accgacgcaa ttacgcaggc aatcaataaa ccggggagta gctgatactc
    42001 cccggtcatt tcacatatca tcaacgtcga cataatcggc gcgtgcgtgg ttgccgccaa
    42061 cagtgtcgcc atcccggtca atccgagtaa aagcgtaatt tcttcgccat cagggaacca
    42121 taatcccagg ctacgaccat acaacatgcc aatggcaaga ccgataaata gcgtcggtgt
    42181 aaagacacca ccgggtgcgc cggaaccgct actcgccagc acggcacaca gtttacaaag
    42241 gaagatcccg gcaatgatca ttaacagtgg cggggcggtt aaaaaggatt gtacggtgct
    42301 atagccgttg ccccacactg caggtgtgaa cagggaaagc agacccacga tcaacccgcc
    42361 tagtgccagt tgccagggcg gcgcaagttt gagacccaca aatccacgat gacaggcgtt
    42421 cattaacgtt aacaacagtg gtccgcacag acctgccagc acacctgtac tgataatcag
    42481 cgcatagtca cgagcctgaa ccgtcactga gagttgtacg ttgtagagta acgcgtcgct
    42541 atgattaatc agattgctaa ccagcaatgc cacgacggcg gaaataatca ccgggccgag
    42601 agaggccaac atcatagtgc caaacagcac ttcggctata aataaactgc cagcaagcgg
    42661 ggcacgatag gccgcagcca ttcccgccgc ggccccacag gcgatccata atttccactc
    42721 ctggcgtggc gtaaaacgtt gggcaaaaca ggaggcggca agggcagcta aaagaatcat
    42781 cgcaccttcg cgaccaattg cactgccgct ggttactacc agcagagagg caagcgattt
    42841 aaccaggctt gctgcgtaat cgaactgtcc atcggtttgc aacgcttcca tgtaatcggt
    42901 cggcgcatga gggcgttgtt gggtaaattt ctgccagccc atcagcaaca aacccgccgc
    42961 cagtccgccg agcgccggag ttagcaaccg tcgccagggg gaaaggtttg tcgctgcatt
    43021 gaccagactg ccggagtcat tattgaggaa caaccactcc agtagcagca tcgcatgacg
    43081 aaacccggca acggcaaagg ccgcgagaat accgacgact gttgcgataa gcagacggcg
    43141 gaacatagtg cgcagatccg gatagatgtg tagatgatgc atgagcagaa atgggcaatg
    43201 aaacacgggc gactattttg cggcaaaaga ggccagccgc aaaagcagtg cgtgctttaa
    43261 gagataaaaa aacgtgacac tgtaacctat tattgccgga ttgcgcgtaa tcgtcaccat
    43321 ccggcaatat tacggtgatc ctatcgaaac agcggtttaa ccgcgacagg aattaacagt
    43381 tgtgattgcc attgcgccag cgttaagcga gccagttcac ccagtgcacg gtagaaaggg
    43441 tgttctgctt tttcgataaa aacatcaaga aaacgtgttg accacggaaa aaggtgccat
    43501 gccagcaatt cttcgcattc tgtctggcga ccattctccg ccaaccacgc agccatcaac
    43561 agcagtgaac caaaatgatc ttccggttcg ttttgcttca tttcaaactg aatgcctttc
    43621 tcgcgcatcc actgacgaag tgccaatgtt gaatcgccaa acagcacaga ttcgcgatcc
    43681 agccagaccg acccccacgg cggagacggc agtgcccacg ggccgacaaa caaacgctgc
    43741 caggcctggg cgtgagtctc ttcacactgt gtctgaaaag ccgtcaccag cggcgctaat
    43801 gacgcttctg gtagaggcca ctgagtttcc cagccgtcac tggtgagtac cgcaaccaga
    43861 ggtgcggctt ccgcactctc tggagcgtaa taaaacagcg cgcccaacac gcgcgccgcg
    43921 acagaaaaat tatcttgctg tgaaaaatgg gtcattccta acatcctgaa atgtgcgggt
    43981 tgccccgcac caatttgtta acctgcaata gccattccta ctgtcatgtg caggccataa
    44041 aagagcacac gcccgatcat ctcaccgcca agtatgagga ttagccccag aataagtccg
    44101 gcaacgtgag gttcacggcg acgaatgagc gggcaaagcc agcatccaag acctgcgcat
    44161 aacaatacca cacgccagac ctgtaaggag gcgtaatctg gtaccagtgc gctggcttgt
    44221 tgcacggatg agtgaataga cgccagagac aaaccttgca gcacaatcac cccggcacaa
    44281 gcgatcagcg ccagaacgct aatgatggca aatggtgtgg tattaaatgt aacgcgggct
    44341 gcccgcagaa ttgcggcagc aagtattgga ccgctcaaca ataccgtcag gaagaaagcc
    44401 agcgtagtgt aaccgttatg ccaggttggc acagtgtcga tttgatacac gcaggtcatc
    44461 atccagacga aaatgacgcc gagcgccatg ctgacaagta accagagttt ccccaacgct
    44521 tgcggcattt taccgatgac cgccaccagc caccatagtc cgccaacggc aaaaaatatg
    44581 gaaccggcag caatctcgtt actcagacca gaagctccga ttcgattcag cgagttaaac
    44641 gcccgcagtg gcgatcccag gtgcatgaca gaggcaataa acccgatgcc catcaacagc
    44701 cagaggaaaa acatgccacg gacaatacgc tgacggtctg cgtcattttt ggcggcgaac
    44761 cagccaattc cgctaacaat cagcgcgcct actacgcatt gacccaatac ggtaaagata
    44821 accagtggcc actcatgcca tccatttccc attttacact tcctccggat tagccagata
    44881 accagtggta tcgccggtcg ggcggctgtt ggcgttaggt ttgataacga tattgggttt
    44941 tgtgaaatgc gcgcgcggca gtggcgcgac ggcagccaga gtgccgtgct tctggcgcag
    45001 ctcttcaatt ggaccgaact caagtgcacg cagcgggcag gattcgacac atatgggttg
    45061 tttcccctcg gcgacgcgcg aataacaacc atcgcacttc gtcatgtgcc ctttttcagc
    45121 attgtactgt ggcgcgccgt acggacaagc catgtggcag tagcggcagc cgatacaaac
    45181 atcttcatca accaccacaa agccatcttc acgcttatgc attgcgccgc tggggcagac
    45241 ttttgtacat gcggggtcat cgcaatggtt acaggagatg gagagataat aagcgaacac
    45301 gttctggtgc cagacgccat tatcttcctg ccagtcgccg ccagcgtatt cataaatacg
    45361 gcggaaactg acttccgggc caagatcttt aaagttgttg ctcaccactt gaaagcgtga
    45421 gcttaactca ttctaattca attgcttatg ctttctttgc atcctcaaaa acctcagggt
    45481 gggacaaatt tgggacatcg tcactcaata tgtcgtcaat tttcctcgcg tgctctgtca
    45541 aatgattagg tgcaaggtgt gcgtacctac gcaccatttc gatagactcc cagccgccca
    45601 tttcctgaag tactgacagc ggtacacctg actgaattaa ccaactggcc caagtgtggc
    45661 ggaggtcatg aaaacggaaa ttttcaattc ctgcccgacg acatgctgtt aaccacgatg
    45721 tgttatagtc caggcgcatc tttctgatgc ttggtgtcat tgtcccgtca ggccttctgg
    45781 cagcagtggt ataaacaaac acccagcggt gatgtttgcc tatttgatca cgcaacacct
    45841 tgcaggcagt gtcattcagt gcgaccccaa tagcgcggtt tgacttactg tcttcagggt
    45901 ttacccaagc aacacgtcgc tgcatatcga tttgttgcca ctccagatta ataatgtttg
    45961 atctcctcag gccagttgcc agtgcaaatt taactacaga tttcagggga tcagaacatg
    46021 catcaatgag tctcctggct tcttcttttt ccagccatct cacgcgtttg ttttttacgg
    46081 caggtatttt gattacaggt gctttttcaa gccatttcca gtctcgctct gcggcgcgca
    46141 gaatagcctt gatcattgcc aggtgtgttg cttttgtttg cgtgctcacc gatttaggta
    46201 tataggctgg tggttctttc ccttttctta atgcagcctc cacctgcaac ttccatctct
    46261 cctttgtttt tcggttatac gctttgctga taactgagta gatcattgcc tccgatatat
    46321 ccttaatcct tattccctcg aaatgctcaa tccagaatgc gattctggat ttatcggaat
    46381 cgatggattt cttgtcggct ttttcctcaa gccatctcag gcaggcttct tcgaaagtga
    46441 catctggcat atcccccagt ctgtctactc gccagagttc tgcttttcgc ttgtcgtgca
    46501 actcctgagc ttgccgcttg tcctttgtgc caagagactc cttaattcgc ttcccgcccg
    46561 ggagcgagta cgaggcgtac catatttcac ctctgcggaa gagtgacatt ttctttcctc
    46621 tgttatgcca tcacccgcgc tcacctggac agtatgcagc ggtgaatgaa gtgctgcaat
    46681 gcaggcttgc cgggttgtga gataaggtga tcttttaccg gaagaatttt ttcgtgttgc
    46741 ctgtagtcgg cccgttcgta tccagttaac agctgttggc cttgatatct tgagaaactg
    46801 acaggcctct ttgagggtga tactgtgtga atccatattt ttgccattaa aaaccgcccg
    46861 aaggcggttg tcagttgatt gatgtacgtc gcatttttcg aaggctggca atatgcattt
    46921 ccttctcaat ttccgcttta atcatgtgta gttcgttgtg gtcgactcgc tcaaattctt
    46981 cattaaatgc accaattgaa gtggctcggg ttctgccgtc gagtcttcgg tagatcacct
    47041 gagtaagagt taccttgcag atttccaccg gatagttgtt ggcatcgacg aaagattgcc
    47101 cgcgctggat caggacgaac accggtagca ttccttaact gtcatatcat ttgcacctcg
    47161 ttgctacggc tatcgccatt gctccccaaa tacaaaacta atttcagcca gtgcttcgtc
    47221 cattttttcg atgaactccg gcaccatctc gtcaaaacca gccatgtatt tttcatcccg
    47281 ctcgaccacg acataatgca ggccttcacg cttcatgcgc gggtcatagt tggcgaagta
    47341 ccaggcatct tttcgagtca cccacatgct gaattgcacc tgggccatgt aagctgattt
    47401 tatggcctcg aaaccaccga gccggaattt catgaaatcc cgggaggtaa acggacattt
    47461 cagctcaagg ccgttgccgt cactgcataa tccatccgga gagcaggcgg tacgcatact
    47521 ttcgtcgcga tagatgatcg gggattcagt aatattcacg ccggaagtaa actcaaacag
    47581 ggctctggcg tcgttctcgt actgttttcc ccatgccagg gctttggcgt taacttccgg
    47641 ggccacaccc gtgcagacct cagccagcag ggtgtggaag taggacattt tcatgtcagg
    47701 ccatttcttt ccggagcggg gtttggctat cacgttgtga acttctgaag ctgtgatgac
    47761 cccgagccgt aatttttgcc atgcataatc tccctgctcg acacctctca cgtcgatccc
    47821 ggtacgttgt aggataattt ctggtgtcat gctgccacct tctgctccgt ggctttctgt
    47881 ttcaggaatc caagaacctt ctctgcttcg gcctgggaca gttctgacga cgcccgaatg
    47941 tagcggcgaa atatctgaga acagagcggc aagaggtcgt catcccacgt tttttccatg
    48001 gaagtaagaa gggcgttaat ttccgacatg gtctcttcgt taaccggggt gatgtcgcgt
    48061 tccggctgac gttctgtagt atatgcggta ttttcgacaa tgcgttcggc ttcatccttg
    48121 tcatagatac cagcaaatcc gaaggccaga cgggcacact gaatcatcgc tttatgccgt
    48181 aacatccgtt tgggatgcga ctgccacggc cccgtgattt ctctgccttc gcgggttttg
    48241 aatggtgcgc ggcgacattc atccatccac tcggtaacgc agatcgggtg attacggtct
    48301 ttgcggtaaa tccgacatgt acaggattca tcgttctgct caaagtccat gccatcaaac
    48361 tgctggtttt cgttgataat gcgggaccag ccatcaacgc ccaccaccgg aacgatgccg
    48421 ttctgcttat cagggaaggc gtaaatttct ttcgtccatg gattaaggcc gtactggttg
    48481 gcaacgatca gcaatgcgat gaactgcgca tcgctggcat cacctttaaa tgcagtctgg
    48541 cgaagagtgg tgatcagttc ctgtgggtcg acagaatcca tgccgacacg ttcagccagc
    48601 ttccctgcca gcgttgcgag agctgtactc atctggttta tacctctgaa tcaatataaa
    48661 tctggtggcg ggcaatggtt tcaaccatgt accggatgtg ttctgccatg cgttcctgaa
    48721 aatcgacatc gtcatcaaat gcacgggcaa tggctttttt gctggctcca tgacgttgca
    48781 gattatcgat gcacagcgat tcaaacaggt gctggggcag acctttttcc aggtcgtccg
    48841 ccagttcagt ctcattttct tcacgggtaa tttgccggta gtgtcgtgcc catgactgct
    48901 cttcaatgcg atcggggata agccaggcat tcatgattta tcacctccaa aattttcaag
    48961 cctgttggca atcatgattg cgatatcagg gattgctggc gctgttgcga cgcatgcagg
    49021 gtttgcgcac aaaccataga cggctgcaag cacgagttgc cttttccagt cgaaatctgc
    49081 ttgctctgaa tcatcattat tgtcagaagc atcactatct ggatcgatgt gaacaaccga
    49141 atcgtgttcc tgagttgtat caacagcatt tttctgaatg tattcattct tcatttgtgc
    49201 ctggtcatct ccagcagcgg tctcattttc tccaaaagtt tcagcgtaag tttcatctcc
    49261 cattactgga ccacagtcag ggcaatgccc gccactgttc tgaccgcatg cggtgcaaac
    49321 cttttccact tcctgttgca ttactggccc aggctgttgc tcttccggtc cgttttgttg
    49381 cgtatccggg ctgttttgtt ccactttgga ttgattctgg tcctcagtgt cgcgagtctg
    49441 gatccccttc acccacttcg ggtcggttgg gtcgctgatg ccttcaacga actccccacg
    49501 agaggcagcc agcaatttgt ctgcatcgac aggatttttg ggcaaaatgt ttttcctggc
    49561 ttcatagagt tctgctctca gttcctggta tttcgtttct acagatgaga cattttccag
    49621 tgattgcgtg tccgcattat gtttaactgg aatttcttcc actgattcag gcgctgcctg
    49681 ttcattagac attgcgtccg atgtttgctg cttttcttca tcgtcatgta tctcttctgc
    49741 tgttccgcgc tgcggcatcg gggctgatga gcgaccgcag gcaatttcca cgatttccgg
    49801 atccgggtta gagtgatcgg tttcggtcaa cactttgttg agatattcag tcacgcgcgc
    49861 cgggatagcc tcaatgccaa ttggtgcttc tttcactgaa gccaccacaa tggcgcggga
    49921 ataatccagc ccaccgggca tggcgatgaa tttgtcacgg aaaacagaaa agggcggttt
    49981 attctctgca acgatttctt caacgcgttt tgcatgtgca gggtgcaggt tataaatatc
    50041 cacatccatt gaacgggcca gaacgccagt ggcaatgtct cgcgccagtg atgcctgatt
    50101 gtgggtaaag ccttcgccac gatcggtaag atttccgccg ccagcgttag caccggaagg
    50161 cgtacgagtg atgcctgtaa cataatttcc tttctgccat tcttttgcca gcaatccctg
    50221 atccacatag tctgttttca tccaggtgga gatgaacttg tcgaattcgg ccgggctgat
    50281 atgacggggt gttgcgtgag agaatgcctt tccaacggat tgtgccagtc ttcccagatg
    50341 ataattcgtc agcttatcca gctctggatg tgaacgcaca gcagtaagca ggctctgaag
    50401 gtaactgtct tctgtgtcca tctccatacg gatcacgtta tttcgttgtt cgggtgtggc
    50461 gtgatgccga tattttccat cttcatcttt gctgaagaag aggtgaagga agcgatgagt
    50521 aaggctcaga gtggcgacgg gaatttcaca ctcagaacag tcatcgtcgc tgtccgggga
    50581 ttcgcttttc tccacatcat ccggaatagt tccgtccagg ttatcgtagt catcgccagc
    50641 tgttgtggca tcttcaccgt tgatgttgtc atcgaagggg attgccatca tggtgatgcc
    50701 atcttccccg cctttttcat agcggttgca gaattcagta tcaaacacgc cttccggtgg
    50761 aaggtcattc acgacgggga aatttacgcg aacgggtttt ttgaaatcat cctcgtcgta
    50821 gcctgcatcg tccatggctg caatgcagcg ggaaactgcg acagaaagtt ttctggtatc
    50881 agcccagaaa aaaccgcctt tgatgccaag gcgttttctg actttatcgt tttttgcttc
    50941 gcagtgcagt gcaaaagttt gtttatcagc gctcattgta tttaaacctc tggctggatt
    51001 agaattagcg gccctttgtc tgatttctcc gaatacagtg acgcaggggg gagtccggta
    51061 gcctgcgctg ccggattttt tatttcagtg taaggttgcc tggtttgttg ttttgtgagt
    51121 tgtttttttc ttttcatgct ggcagtcaga gcagttgcat tcgccatttt ctctgagaag
    51181 ttgaagtgcc agggtcagtt cctgaattct ttgtggatca atatgactgt cgacaatttc
    51241 agatgcagca tgaatatatg ggctggtggt ttcgcaatat aaaccgaagg aaacaaagcg
    51301 actgtcttta tattcgttat ttccaacttc tgtaagcact gaaaaagaaa atacaaagtc
    51361 gattttgtgt tttttgcata ttttactgat gcgttctgca atttctttga gttcatttat
    51421 tgcatcagga ctggtttcgg aaaaaaattc attttgcgca agttctttca ttttgatttt
    51481 cccgtgattg ataattaatg tggtttttat ttcggtttgt ctgttacaga tatggaaggt
    51541 gtattttatt actcgtcaca actactctgc ttttacaggt aagccatcgc gcccgatgaa
    51601 aactttaatc atgcagtcgg taatgcatgt ttttgtagtc aggctacgaa tatagagctt
    51661 tctcttttca atattgtttg ctgaagcgat atatgtgcgg ccttcatgaa gaacataatc
    51721 gccaggtttc acgcactgac gtggtatttc atcagttccg aagtgatgag caatcataat
    51781 tatctccatt ttcacaaatg aactttgttg atgcggtgcc tagtgcctcc aggtgacgtt
    51841 aaccagttaa caattaacgc cggagcaggg gatgatgatt ttccgtgaca tcctgtcggt
    51901 ttaactgttc cgcgtgcgca tagccgcatt caccgcatca taaaattcac ttttaaaaag
    51961 ggcggacatc agcaatcggc aaaccgatgt ccgccaaaga ggatattgat acatggatgt
    52021 tgcagcgggg ttgtcactca ggcgtctggg gcgatttcca ttatttacga tgcacttgaa
    52081 acattccttg atcgaaaact ttcatctgtc accgacggaa catcgagtaa gcaataacat
    52141 ttaacacaca ctctaaataa caaggggtgg gcttgatacc gttcttttca agctcactcg
    52201 taatcacctc cagaagtcgt gcgcccatga gaattgtctt gtgggatgaa gctattacgc
    52261 cgttgctgat attgctgttt atttctattg cttcattttc accattcgcg ctaacttcgg
    52321 atatgttatt ttcactctcc tgtttcagag ctttttctgc catccggatg cgttcctgcg
    52381 ttgcggagtt tgggctaagt cgataaacct gtctggcatc ttccagaagc agggcgataa
    52441 tgtgcttcag ttctgtttcg ttcatagtta actccggtag ttgcaattta ttaatatcag
    52501 gcggtcagct ctttaagctt ctgaactgct ttattcattt catccataca gtcgatgaat
    52561 tcgtccagtt taagctgcat ttttccggcg gcctgaagaa tttcaagttt taagggcgca
    52621 agttttttgt tgaatacatc atcttcctgg cgttttttct ccgcaatttc ggcggctttc
    52681 agcaattctt ccgcctgctt tctgagtatt tctggtgaag gtgaaactgc ttttaggtta
    52741 ttcatttcag gttcctgttg ttgcagaatt aatttgttgc attctctgcg tgagcgtttc
    52801 atcgcagatt cgcacttctt gcaataaatg gctctcccgt cacgtttgct ggcatctgaa
    52861 taaaaatcat cgactgattt tgtctgcccg catctggtac ataattttga ttgaaggtcc
    52921 ttaatgattg tgacgtttcc ttcccgcttg ggatatccat attttttgtg gcggaaaaag
    52981 gttactccgt taccagaacc ttcttttctg accttaaata caatgatgct ttcccgttta
    53041 tcgataaaat ctatgagctg gtttctctcc tttcctgaca gttcggcaaa tgtcttaact
    53101 tttttagtaa cttccgtaag gcgaacgccg tcaggcattt tttcaacgaa gtttttaatc
    53161 tctgcaagcg gacgccataa tttttgcggc aaaactgaag gcgtggtcat caatcacctc
    53221 gctttttttt actgatttgt ttatgccaaa aataaaggcc accatcaggc agccttgttt
    53281 ttctgtttgc taagttctct ggcaatcatg gccgtagttc gtattgccca caccattgat
    53341 ttgtatcaat agtcgtagtc atccggatag tcctggtatt gttccatcac atcctgtgga
    53401 tgctcttcga actcttcaaa ttcttcttcc atatctcact tcaaataagt ggtttgctgc
    53461 ctaattttat tttctggcga ccaacacaag tcacacccgt ttcactgcgt ggcttgcggt
    53521 agtaaatttt gttagtccaa acaataaaat ctaccgaagt gggttatgac cattttttat
    53581 ttggatttcg ttggtgagcg tggttaacaa ctctgtgcat tatatcctca tatttttcat
    53641 ctttaatttt ttcaacatct cgaggaaatg gtgttgctaa tgctttgtca actttgtcca
    53701 ttgggtcttc attaatctta tattccggac cgtcatctat agcattaaat ccaggtgtta
    53761 taccgttttc taatgcatat gctattctct tttcccatct cgctatcctc cttctgtctc
    53821 gagatgtaag gcttctatca gatacttttc tgttttgtcc gcgattagga ttaacataaa
    53881 tagtcttttt caccataagc atactcaata agtccatacg gtggtttact gtacaatttt
    53941 attttttgaa ctgcatgtat tttgtttcct aatgggtttg aatccttgta ataagtactt
    54001 ctgtttttgc gctcgacttc ttctgccttc ttgttgcgaa ggctgccgag tgatgctgct
    54061 ttgtctgctc tgacgcaacc agagatcttt agcgcaatct tccgcgtcag tctttcacta
    54121 ctgcgccgct ctgcaataag ttcttccctg cgagctttat agcggctttt tgccgtacct
    54181 ttggattctt tccagattat ggttaccatg atggtctcct ttaagtggct ttggtgtatg
    54241 acgcgtcgag gtgtttttct tctcgatcgc ggccttgcaa ctgaaattcg cgtcatcccc
    54301 aaaaccactt agattttggt ctcaacggtt aggttgagag tccatcaatg ttaaagagcc
    54361 tgccaatctg ttccgtttgg cttccagcgt cctgctgttg aattgaagat aacctaagtt
    54421 atctgattgt gcaataacta tatttatcat tttatggaaa acgttataaa tggctgataa
    54481 caaaaatatt ttattttttg tggtatccgc gtgatatttt caagggggaa aggctgattg
    54541 ttatgggtga ttgcatgtta atcgaagggg aatttggtgt gttgcaccag cgggtagtcg
    54601 aaattctagg ggtgtcgttg cttgaggtta ttgctactgg ggaagctatt tcagcagatg
    54661 ctattgcggg aatgatccga gtgcttcacc atgatgaatt ggatgatctc gctgtgaagt
    54721 tagctataga tgtgttactt caggatatgc gactgtgtaa ttaagtaaat aaaacccggc
    54781 actggagccg ggggatttag aaatggcatt ttatgaattg gctacttggc gaatggttta
    54841 ccatcggtgg tattgagcac aataaaaggc cgttcttcgt gagtgttctt atagcaaaag
    54901 ttcagttgga gtctgattac cagctatgtg atgaccagaa cactcgtcct atgattttta
    54961 cgtttttgta gaattcttct ctgttcatta cttcatcagg atactcttcg cggtttatcg
    55021 atctgataat taccgatgtt ggtgtggcta ttagcgtttt tacccttaac aaatcagctt
    55081 ggcaaattgc atatgtttta ccatccctga tgctcgtatc ttgcgtgttt acccccacca
    55141 catcaccatc atggagtgtt ggctccatac tttgcccaac aaccctgacc aactttgccg
    55201 ctttttctgg aactcccatt tttttcaggt aatagcgcct aaaaactaga gagaattctg
    55261 cggactcctc caatgcacaa cttcctccgc cagctgaaag tgaaatattt agaaggggga
    55321 gcgcaacaaa ttcgtcatta tcattttgat gatcatccca ggcgatagct tttaaagatg
    55381 attcccgagc attagatggc tcttctgcgc tctgtggtct cattgaccct ataccagagc
    55441 ttagccactc agggcgaact cttaacgcgt tggctaattc gaccatttta cgtgatccgg
    55501 ttgttttacc agatgacatc ttttgtatgg ctggttgtga tacccccacc atgtctgcaa
    55561 gttgtgcttg tgacaacccg gctgaactca tggcggcatt tagtctttct gcgaatgttt
    55621 tcatacctgt aatctataac cacggttatc aaaagtaaaa caacaattgt tattgctctg
    55681 ttgtataact catgttattt ttggttatgc tttatttgct gtagaggtat gctcatgaat
    55741 ttagtgattc agcgagcctt gaatattgtt ggcagtcaaa aacgacttgc agctgattgc
    55801 ggcgtatcac agcccgctgt tcataaatgg ttgcgaggcg gaaaagtttc tcctgaaaaa
    55861 gttttcgcta tcgttaatgc caccaatggt caggttaagg cttacgaaat tcgcccggac
    55921 ttaccgcacc tgtttcctca tccgaaccag gctgaataag taacaccgct ctttaacatt
    55981 gctggtcgtt cacctctaac agggtgagca aacatcagtg gcaaacccat tgggattgcc
    56041 gcttaacccc atatcaatat aggaaaatta acaaatgtca caaacaagtt acagcaaact
    56101 gtcgcagcgc gatatcgatc gcgctgaaac ggatttactt atcaacctgt cagttctgac
    56161 gcaaagggga ctggcgaaga tgattggctg ccatgaatcg aaggtcagtc gtaccgactg
    56221 gcgatacatc gcggcgattt tatgtgcgtt ccagatggca tctgatatca gtccgatcag
    56281 ccgggctttc cagcatgcca ttcgcgttca tgcaaataaa aaacgtccgg ttggggccgg
    56341 acgttctgag caaatcctga tgaatatctg atattcaggc agggcatgga gcaatacacg
    56401 ggaataattc tgccacatct ggaagaattt cgccagcaac aacaccaacc gcagcagcct
    56461 gaagccgatt gggttaaccc gggagatacc gggaccgtct gcggtatgga gtaaatcttg
    56521 tatgcgaggg gactatgcgt aattacgcaa caatttcacc tcagttctgg ttaggcgata
    56581 cagggcgaaa actaaggaag tctggtccgg aatgtatggt agtggcgttg tatatgatga
    56641 cctcgcctca ttccaatatg ctgggccttt attacctgcc tgttttgtac attgctcacg
    56701 aaaccggact tgatcctgaa ggggcttcta aggggcttca aatggcttgc gaggctggtt
    56761 tttgcagcta tgaccatgat tctgaggttg tatgggtgca tgaaatggca gcatggcagg
    56821 ttggtgaagc gctgaaacca ggcgataacc gttgtgctgg ggtaagaaat gaatattccg
    56881 cgttgctgga aaatcctttt ttatcatcct tttatgatag atataaggat gatttccacc
    56941 tggatgtcag acgtgaatca tgtcggaaaa ttgaagcccc ttcagagccc ctttcaagcc
    57001 aagaacagga acaggaacag gaacaggaaa gagataaaac ccttctggtc catggcgaaa
    57061 aaatcgccac ggacccactg gtggattctt gtcccgttct gactgaacgt ccaggaccag
    57121 ctggcatgac accggaagca gattccgggc gttgtgtgca gcaggtgctg cacgtcgaac
    57181 cggagcaaca acaccaaccg cagcagcctg aagccgattc cgcgatgagc gggaagccga
    57241 ttgggttaac ccgggcgatg ccgggaccgt ctgcgggacg agttgattat cctgacgtgt
    57301 tcgaacgggt ctggcgtgaa tatccgcatc gggcagggtc aaacccgaag aaatccgcgt
    57361 tcaatgcctg gagggccaga ttacgcgaag gggtgtcacc ggatgtcgtg ctggatggcg
    57421 tgaggcgtta cgcaagatac ctggaggcta ccgggaaagc gggaactgaa tttgttcagc
    57481 aggcatcgac gttttttggc ccgaacagga atttcgaaaa tccgtggtcg ctgccgaagg
    57541 ctggcgcagt cagcctgcgt tgcgtgaatc acatttctga accggacacc gaaattccgc
    57601 cgggtttcag ggggtaatca gccatgaaaa acatttcgac aggagggatt cttgaacggg
    57661 tgcgccgtct ggttccaccg cacgttacag caccgttccg gacgaccgac gagtggctgg
    57721 aatggcaact ggctgagggc cggaagcgca gcgaggaagt taaccgccag aatcaccaga
    57781 cgcgggttga aaaaatcctg aatcgggcat ccagccgctt cacaggaagt gctcattcgg
    57841 gaactaccgg gtgcagaacg acggtcagcg ccatgctctg agtcaggcaa aatccatcgc
    57901 ggcagagctg cataccggct gcacgaattt cgtgttcagc ggtaaacctg gcaccgggaa
    57961 aaatcacctt gcagcagcga ttggcaactg gctgatggcg aaggggagaa gcgtgattat
    58021 cgtcaccgtg tccgatgtca tgagcgtgtt gcatgacggc tacgacaacg gcaagtccgg
    58081 ggaaaaattt ttacaggaac tttgcggggt agaccttctg gtccttgatg aaattggcat
    58141 gcagcgggat acgcgcaacg agcaggtcat actgaaccag atcgtcgacc gcagaacggc
    58201 atcactgcgc agtgtcggga tgctgacgaa cctgaatcac gcagctatga gcacactcct
    58261 cggcgagcgg gtgatggacc gtatgaccat gaacggtggt cgttgggtga attttaactg
    58321 ggagagctgg cggtcaaacg ttgggcgtca gggtatgtga gaatttttga cgaggtaaat
    58381 tttcgatgga aaccgtattg catgcactga aagcgatggg taaagccaat tctgttgaac
    58441 tggcggcgcg gcttgatatc agccgtgaag aagttctcaa cgaactgtgg gaactcaaaa
    58501 aaaatggtgt tgttgataaa acgggtcaca cctggtttct ggctgtcgaa ggtgaatccg
    58561 gggtaaccga agggcaggca ctacaatctg aagcgccgga tgtggtaacc gaagaggtcg
    58621 ctccaaaagt tagcgctgac atgatgattg agtttatcgc tcaggagggg gctaaaacct
    58681 gtgaagaaat agcgggtaag ttcggagtta ccactcgtaa ggttgcttcc actttggcgg
    58741 tagtaacagc aacggggcgc ctggtgcgcg tgtctatcaa gggaaaattc cgttataaag
    58801 tgcctgacgt cgaaaacggc gatttgagcg cgtcatcatc gcaggatgag aatatcacgc
    58861 acgaagcaga aaacagcgcg tcaggcgacg aactgaatcg ttttccgttg atgtcagagg
    58921 gcacggcaaa ggcctgtaca gatgagttta tccgggatgt tccgtcgttc accgaaaaat
    58981 atgctggtgc agtgaccctt ccacctctac gggccgccag tcgtgaactt cgtcgcgcca
    59041 aaaaacaggt ccagaagtgg gagcgtgtct gtgctgcact acgtgagctg aataaacacc
    59101 gggaacttgt catgcagatt accgattcat ccagcagtat tgcatcggaa aagtgataac
    59161 tggagagctt atggcaaaag tatttacacc cgaacagcgg gaagaactga aggcacgtat
    59221 tgtggagctc gtgcgcctga atggtcgggg aacgttcagg caactggcta ctgaaaccgg
    59281 agtcagcaag acagctgtca gacgtttgtc cagagaactg gttgtcagtg gcgctatatt
    59341 cgactcaggt tatggtttat tcccgtctga tcaggcgcgt aaggactggc aaaatgcccg
    59401 caaaaaactc tcgagagcaa aggcaaagaa accggttgtt attgaccctg gtcttatccg
    59461 ggcattacct gatggggaaa tccggcgcta cgacagacgc tacaacataa tttgtcgcga
    59521 gtgccgtaac agcgaaacga tgcagcgtgt gctggcattc tggcggggga gtgcagaggg
    59581 attgatgttc tccccgtcgt gaatgacggg ggcttacgtg ttcagaaaag tgattcatat
    59641 agaggctgaa tctgatcttt tttgttcacg tctggctgcc accattgcag gcagagtgaa
    59701 ggcgtagact aaaaacattt cggtaaaact caggatctgg cttgcctcaa ttggtgtgaa
    59761 aacttcatct gtatgaactg ctttattggc atcaattcgt acaatatgag cccattcctt
    59821 catctgttca gtgatcagcc cttttttgta aatcatctga atacgttgcg acagcgattc
    59881 ttttcctgct tcatcgccga gcagtttttt cgtggagata tcgaggactc tgcgacaaag
    59941 aattaccact gtgtcgtacc gcctcctctg taaatcctct tttgcctcaa caaaagtacg
    60001 gtctgcaacc gggggcgtat attctggtgc ggtaattttt ttgaccgcag gatagatctt
    60061 gcggaaacgg tactggctat ttcctgaaat cagaatatcg aggtcttttt tctggctttc
    60121 cgccatgggg ccgtgatagt ggtctgatgg gatttcaaca atagcaacac ctcctcgatg
    60181 acaacttctg catacaaaag cgacgttaaa aagaggtgtt ttttcaattt gtttttcggc
    60241 aaatgcctca ataacagctc gctcttttaa acagtgtggg catgtaatgt caaaggagac
    60301 taaactcatg gatattcctc tcgaaattaa agaaattaac gaaatcattg aggttactga
    60361 tcgccccgag tttaccctca tgcgtcgtta tgaaacagga accgacgaac aaaaatacat
    60421 catggttgca gcattggctg tgatggcgat agaaagggag cggagggaaa aggacgtaat
    60481 ggttattccg gcgagaaatg attcaccgga tttgagatgg caggatccgg agtgggatgt
    60541 ggcaatccgg tgattgatat ttctctgggg taaagcgccg ccagaatgac ggcgcggtgg
    60601 ttaagcatga ggcgaaattt ctgccgggcg gtattttgtt gagagatgag atacgtcaac
    60661 ttctctttcc gctttttcaa ggctccatgc cgtctggaga cgaatccatg aagacggagt
    60721 tccacccagg acagcagcga gacgaacagc catttcagga gatattgtcg cattacctga
    60781 aaccagtcgc tgaacagtgg aaggcgcgac atcaagcgct cttgccagtt ccctgattcc
    60841 aatattctga tcttccagaa tatcagcaat aatttctccg ggatgagggg ggttaaacat
    60901 cgccattagt ggtaatcctc gtagttaaga atataagcat cgccgttccg aaactcgaat
    60961 gtgattctcc agttaccgga gacggtgaca gaccatgtat cggatctgtc accctgcaac
    61021 tggtgcagcc tgtacccggc aaggttaata tcatcaatga tttcagcttg atctattacc
    61081 tgaaggcgcg ttctcaggcg tttaacgtga ttgggattta cgcctgatgc atttcctgtt
    61141 tcaaagaaac gttgaagtcc tttgtgtttc cagcttttta tcatgcttgt ttcctgttgc
    61201 gttacgcgaa tcattatagc gactgttgtg taatgcgcaa cggttgtttg acaaaaatag
    61261 gtatccggga ttatattctt cgcacggtcg agttgaccgt cgggattgga accccggata
    61321 gagaccgcga caaacacacg ccgcgagcgt gttttttatt gtcgtatgca cgcgcacatc
    61381 tgaattatgg tggggcgcat gggggagctg aaaagctcgc cggtcggttt cccggtagtt
    61441 ccaaccctgt gcgtctcacc acccgatgat tggaacctta cggtggtgat agtttagaaa
    61501 ccactagagg gcgtcattat gacaactcaa gtttctgttg aaactctttc cccgatcacc
    61561 cacaaccaga tcccagttat tacaactgaa cttcttgccc agctttacgg cacagatgta
    61621 actaatattc gcaaaaacca cagccgaaac catgaacgtt ttgttattgg taaacattat
    61681 tttttgctgg aaagtgcaga attgcgtgag tttaagcaca gagtgtcttt aagtcactct
    61741 gtgacaatcg cccgtaacgt tcgctccctc atcctctgga cagaacgcgg cgcagcccgt
    61801 cacgcaaaaa tgctcgaaac cgatcgggct tgacgttata ggtcgttact tgtagattta
    61861 tctttgtgag gcgtcagaac ctctctaaag cggacaaaac caaccccgtc agtgttggat
    61921 tttttatgcc tgttattcag tgatagcaca atgtgctgtc acatccccga acaatgtcgg
    61981 gagggcgacg aatacaacac ccgtaagggg aataagtccg cggttcttta ggccgttctg
    62041 aacctcccga caccactatc agaggtggta atttcagaaa aagctaaagg aggtcgttat
    62101 gaccatgcaa ctcgctgtag aatccctcgt cgtcgttact tactctaacg tccctgtaat
    62161 aacaacaggg ttattggcaa atctatacaa aactgaagaa aaacatatac ggcagaactt
    62221 caaacgtaat gaatgtcgtt tcattgcagg aaaacacttc tttaaagtaa gtggctgtga
    62281 acttgatagt ttgcgaacct ctcaaagagg tttacaaatt tccccaaaaa cccgctccct
    62341 catcctctgg acagaacgcg gcgctgcccg tcatgcaaaa atgctcgaaa ccgatcaggc
    62401 gtgggaagtg ttcgaaaaac tggaagactg ctatttcagc cagggaaaaa caacacaaac
    62461 cgaacagcag tcgcagattc agccacaatt cacagccgag gaaatcatcc tcctttgcta
    62521 catgcagctc tggatggaaa aagcccagga cctcagcaaa cacctgtatc ccatcatgaa
    62581 agagctgaat tcatcataca cgaacaagct gtatgacatt acgtttgaga ccatctacat
    62641 ggtgacgaag aacagagacg cgctactgcg ggaggcggca cggcttgacc agtcaagttt
    62701 cgttgtccag cgggccagac caatgctgaa aagcctgcgg gcaagacaat tcgagtttta
    62761 aggccagatt tgacaaatcc gcattagcgg ggatatattc cgctccatgg tgctgaacac
    62821 accttcgaaa gcggaaaccg cgcccgtcag tcatgcggat tttttatgtc catttttcag
    62881 atatggtcgg gtagcgcgta taccgaaaaa cagccgcaag gctaaggatg cgggccgaac
    62941 tttcgacggt gttcaagtac ccgaccgccc tgctgaacac ggggtgatct gaacgaatcg
    63001 aaaggacata aaactatgag cactcaactc gtattcaaat cccacgttct tgaaacgatc
    63061 gaacacgacg gcaaacagtg gttcactgcc gcgactttgg cttctgcact ggagtattcg
    63121 cgcggcgata aaatcaccca gatatacaac cgcaattcgg atgagtttac tccttgtatg
    63181 tcaatcaacc tcaaaatgag gttcaacggg atcaacaata gcttacgaga gaaagatgtg
    63241 cgtattttct ctctacgcgg tgcccacctg gtagcgatgt tcgcaacaac tccagtagcc
    63301 aaagagttcc gtcgctgggt attggatatt ctggaccgtg aagctgaaaa aagcgctata
    63361 ccgccacaac ctcatattca gccgcaattc acagcagaag acatcatcct gctctgctac
    63421 gttcaactct ggatggaaaa agcacaacaa ctcagtaaac agctataccc cgtcatgaga
    63481 gaactgaact ctgattacta cgggaaactg tttgatctgg cctacgagac tcgtcatata
    63541 accaaccgga ctcgcgacac attgctccgc gaagcagcaa aacttgatcc cgccaactat
    63601 atggtgcata gggccagaag catgctggcg caactacggg caagacaatt tgaattctga
    63661 aaccaaagga gcttcggctc cttttttgtt gggaaaatcc agtgagaggg aataatgaac
    63721 cagactatct tcctccgaag taagcagcag caacaattcg ccattaacgc aatccttgca
    63781 acaactctcg ataaagacaa acccgttacg atccgcatca ccgattacaa gcggaatctc
    63841 gatcaaaatg ccaaatttca cgcgatggtc gctgatatct cccgacaggt tcagtggtgc
    63901 ggcagatggc taaaaccaga acagtggaaa gttttgttaa tcagcggtca tgccgtggcg
    63961 acaaaacagg aagccgatgt tttacccggt cttgaaggcg aatgcgtcaa tatccgcgaa
    64021 agcagtgcgc agatgagcgt gaagcgtatg gcaagcctga ttgagtacac aacttcctgg
    64081 gctgtggaga agggggtcag atttaccgac aggaggtacg aatgagacga cagcgacgaa
    64141 gcattaccga tatagtctgt gaaaactgca attaccttcc aacgaaacgc tcccgaaata
    64201 aacccaggcc aatccccaac gaatctgatg taaaaacctt caattatacg tctcacctgt
    64261 gggatgtccg gtggctcaga cgtcgtgcaa gatttaatag tcactccgga tagttcaatg
    64321 tacgaggaat aagatgatgg caaacctacg caaagaagcg cgtggcagag aatgtcaggt
    64381 acggatttac ggcgtatgca atggcaatcc tgaaactaca gttttggcgc attaccgaat
    64441 agctggaatt tgcggaacgg gaatgaagcc agatgacctg ttggctgcat gggcctgtag
    64501 tgactgccat aatgaaatcg atcgccgtac tcgcattctc gacaacaacg acgccagact
    64561 ttaccacctg gaaggcgtga tcaggacgca ggcgatactg ctgaaggagg ggaaaattaa
    64621 gtcatgaatg aatatgagtt tgtgcttccc tggccgccga cggtgaatac ctactggcga
    64681 agacggggaa gccagtacta catcagcgat aaaggccaga aataccgaaa agacgtacag
    64741 caaatcatcc ggcaactcag attagacatt ttcactaaat cacgacttcg catcacaatt
    64801 attgctgaac caccagattc ccgccgtcgc gacctcgata acatcctgaa aggtttactc
    64861 gactctctta tctacgccgg atttgcggaa gacgacgagc aattcgatga cattcgcgta
    64921 attcgcggcg tgaaagtgcc tggcggtaga gtggggataa aaatcaccga actggagaac
    64981 atttgatgaa tgctaaaatt caaacgatac ctgaattact gatctgcacc aggggaaatc
    65041 agacagaagt cgccagaata ctgaactgca atcgtgccac agtcagaaaa tacattgatg
    65101 ataaagatgc gaaaaagcac gccgtcgtca atggcgtcct tatggttcat cgcggatggg
    65161 gtaaagatac tgatgcgtga tatccggcag gttcttgagc gctggggtgc atgggcggca
    65221 aataactatg aggatgttac atggtcgccc attgctgcag gatttaaggg actgatcccc
    65281 gaaaaagtaa aatcacgtcc acagtgttgt gacgatgacg cgatgattat atgcgggtgt
    65341 atggctcgcc ttaacaggaa caacagcgat ctgcatgact tgctggttga ttattacgtg
    65401 ttgggggaga cattcatggc gctggcacgt aagcatggat gctctgatgg aactatcggt
    65461 aaaaaattac agaaagcgga gggtgtggtt gaagggatgt taatgttact tggtgtcaga
    65521 ctggaaatgg accgatatgt gcaacgcctc tgacaggagg cgtttgccgt tcactatgtg
    65581 aagtgtgtca atttgtgagc gttatgctgt ttactttata gttgcggaac gtaagagtta
    65641 tcctcctgct aagcgcagag atgcaacaat gaacaggggg gcaaaatgga cattgaagat
    65701 tttctggagg cagaagacaa tgatctgtct ctgtggaatg atgatatttt aagcattgac
    65761 tggttcgtta ttcttagaga gctgttgaaa tatcagagaa gactaaatat cacccattca
    65821 gaattagttt tgctggctaa tttggtttca tttcaccaga atgtggattc gcaaatatta
    65881 ccgtcaatat ctcttttggc taccagaatg cgcgcctccc gagccattat acaaggacgc
    65941 cttcaggaac tggaggggaa aggaatgttg cagaagataa gttacgctca aatcggtcat
    66001 gatgatgatt tgcggaacgt ctatgacatc aagccattaa ttcgtaagtt aattggggtg
    66061 cagaaaaccg agaatgataa aaagcacact tgccccgtct gtggaaaagt aggttcctct
    66121 gatgaagaaa ttgagaaatt ttttggattc agaaaaagtg gggattataa atggccgcaa
    66181 agctggtgcc gttcatgcag aagcacgaaa caacgccgac tgaatacatc gctaattggt
    66241 aaaaaaaggc cccgcaaatc cccaccagag gtggtgtgac gtgtcgcgga atccttgatt
    66301 ccgttcccga ttgaatgggg tgctaaaaag tattttacgt acgtaaaaat ctgcatatca
    66361 tgataagagt ggttgcattg ccacgcagcc gaccccgtcg atgagcgggg tttttttgta
    66421 cccagaatcc tctgagctat acggaaagta cacagaaagg aaggtgcgac cacaattaat
    66481 aacaaaatct taaaaatcgc acatggcact attagttttc taaatattgt gt
//
LOCUS       DAZFQD010000010        65428 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.010, whole genome shotgun sequence.
ACCESSION   DAZFQD010000010 DAZFQD010000000
VERSION     DAZFQD010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 65428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65428
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.010"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..426)
                     /gene="folK"
                     /locus_tag="ACHKTW_000716"
     CDS             complement(<1..426)
                     /gene="folK"
                     /locus_tag="ACHKTW_000716"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HFO6461787.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESHILAVSSFYRTP
                     PLGPQDQPDYLNAAVALETSLAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELAFPDGE"
     gene            complement(423..1820)
                     /gene="pcnB"
                     /locus_tag="ACHKTW_000717"
     CDS             complement(423..1820)
                     /gene="pcnB"
                     /locus_tag="ACHKTW_000717"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:1990817 - poly(A) RNA polymerase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HFO6461788.1"
                     /translation="MFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQHAISRKDI
                     SENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKLFRNCRLV
                     GRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGSIEEDAQR
                     RDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLRAVRFAAK
                     LGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYDTYKLLCEYHLFQPLF
                     PTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLLETAQKIA
                     QESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQGKRAWKLL
                     EHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDEEPSPRRR
                     TRRPRKRAPRREGTA"
     gene            complement(1880..2776)
                     /gene="gluQRS"
                     /locus_tag="ACHKTW_000718"
     CDS             complement(1880..2776)
                     /gene="gluQRS"
                     /locus_tag="ACHKTW_000718"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HFO6461789.1"
                     /translation="MTDTQYIGRFAPSPSGELHFGSLIAALGSYLQARARQGRWLVRI
                     EDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHHAYREALAWLHEQGLSYYCT
                     CTRARIQSIGGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDQLRGIIHADEKLARED
                     FIIHRRDGLFAYNLAVVVDDHFQGVTEIVRGADLIEPTVRQISLYQLFGWKVPDYIHL
                     PLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFSVEQILQSAVK
                     NWRLTAVPESAIVNSTFSNASC"
     gene            complement(2843..3298)
                     /gene="dksA"
                     /locus_tag="ACHKTW_000719"
     CDS             complement(2843..3298)
                     /gene="dksA"
                     /locus_tag="ACHKTW_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HFO6461790.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3476..4180)
                     /gene="sfsA"
                     /locus_tag="ACHKTW_000720"
     CDS             complement(3476..4180)
                     /gene="sfsA"
                     /locus_tag="ACHKTW_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HFO6461791.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATPGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGAELSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLPVTL"
     gene            complement(4195..4725)
                     /gene="thpR"
                     /locus_tag="ACHKTW_000721"
     CDS             complement(4195..4725)
                     /gene="thpR"
                     /locus_tag="ACHKTW_000721"
                     /EC_number="3.1.4.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004113 - 2',3'-cyclic-nucleotide
                     3'-phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0008664 - RNA 2',3'-cyclic
                     3'-phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HFO6461792.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTIPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            4799..7228
                     /gene="hrpB"
                     /locus_tag="ACHKTW_000722"
     CDS             4799..7228
                     /gene="hrpB"
                     /locus_tag="ACHKTW_000722"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HFO6461793.1"
                     /translation="MSSLPVAAVLSELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGPNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERRYLPLPAHQRFDDAVAVATAEMLRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVLLCPLYGALSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFDPRTGLTRLITQRVSQASMTQRAGRAGRLEPGICLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPVVNLLAAKRL
                     LQMLGALEGERLSAQGQKMAALGNDPRLAAMLVSAKNDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIARRRGQDG
                     RYQLANGMGAMLDANDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLAALETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNLPALAVRMQEMFGEATN
                     PTIAQGRVPLVLELLSPAQRPLQITRDLSAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            7424..9958
                     /gene="mrcB"
                     /locus_tag="ACHKTW_000723"
     CDS             7424..9958
                     /gene="mrcB"
                     /locus_tag="ACHKTW_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HFO6461794.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYD
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDSRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTDTWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            10178..12421
                     /gene="fhuA"
                     /locus_tag="ACHKTW_000724"
     CDS             10178..12421
                     /gene="fhuA"
                     /locus_tag="ACHKTW_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HFO6461795.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAARQSATGTKTDTPIQKVPQSISVVTAEEMALHQPKSVKEA
                     LSYTPGVSVGTRGASNTYDHLIIRGFAAEGQSQNNYLNGLKLQGNFYNDAVIDPYMLE
                     RAEIMRGPVSVLYGKSSPGGLLNMVSKRPTTEPLKEVQFKAGTDSLFQTGFDFSDSLD
                     DDGVYSYRLTGLARSANAQQKGSEEQRYAIAPAFTWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGAKNNTYSRNEKMVGYSFDHEFNDTFTVRQNLR
                     FAENKTSQNSVYGYGVCSDPANAYSKQCAALAPADKGHYLARKYVVDDEKLQNFSVDT
                     QLQSKFATGDIDHTLLTGVDFMRMRNDINAWFGYDDSVPLLNLYNPVNTDFDFNAKDP
                     ANSGPYRILNKQKQTGVYVQDQAQWDKVLVTLGGRYDWADQESLNRVAGTTDKRDDKQ
                     FTWRGGVNYLFDNGVTPYFSYSESFEPSSQVGKDGNIFAPSKGKQYEVGVKYVPEDRP
                     IVVTGAVYNLTKTNNLMADPEGSFFSVEGGEIRARGVEIEAKAALSASVNVVGSYTYT
                     DAEYTTDTTYKGNTPAQVPKHMASLWADYTFFDGPLSGLTLGTGGRYTGSSYGDPANS
                     FKVGSYTVVDALVRYDLARVGMAGSNVALHVNNLFDREYVASCFNTYGCFWGAERQVV
                     ATATFRF"
     gene            12472..13269
                     /gene="fhuC"
                     /locus_tag="ACHKTW_000725"
     CDS             12472..13269
                     /gene="fhuC"
                     /locus_tag="ACHKTW_000725"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HFO6461796.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRGGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            13269..14159
                     /gene="fhuD"
                     /locus_tag="ACHKTW_000726"
     CDS             13269..14159
                     /gene="fhuD"
                     /locus_tag="ACHKTW_000726"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HFO6461797.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPLLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSPEMLARIAPGRGFNFSDGKQPLAMARKSLTEMADLLNLQSAAETHLAQYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRAGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            14156..16138
                     /gene="fhuB"
                     /locus_tag="ACHKTW_000727"
     CDS             14156..16138
                     /gene="fhuB"
                     /locus_tag="ACHKTW_000727"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HFO6461798.1"
                     /translation="MSKRIALFPALLLALLVIAATALTWMNFSQALPRSQWAQATWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPGAMASQFAALVGACVVGLIVFGVAWGKRLSPVTLILAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVAAERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSLGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLCRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQIR"
     gene            complement(16173..17453)
                     /gene="hemL"
                     /locus_tag="ACHKTW_000728"
     CDS             complement(16173..17453)
                     /gene="hemL"
                     /locus_tag="ACHKTW_000728"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HFO6461799.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYYGVVPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGVHETLDELTSRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            17619..17666
                     /gene="yadX"
                     /locus_tag="ACHKTW_000729"
     CDS             17619..17666
                     /gene="yadX"
                     /locus_tag="ACHKTW_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YadX"
                     /protein_id="HFO6461800.1"
                     /translation="MERASKMPSSYLYDQ"
     gene            17678..19099
                     /gene="clcA"
                     /locus_tag="ACHKTW_000730"
     CDS             17678..19099
                     /gene="clcA"
                     /locus_tag="ACHKTW_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HFO6461801.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMVLGREGPTVQIGGNIGR
                     MVLDIFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            19123..19188
                     /gene="yadW"
                     /locus_tag="ACHKTW_000731"
     CDS             19123..19188
                     /gene="yadW"
                     /locus_tag="ACHKTW_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small protein YadW"
                     /protein_id="HFO6461802.1"
                     /translation="MAIIIGLEFAQLPMSFGAKYE"
     gene            19181..19525
                     /gene="erpA"
                     /locus_tag="ACHKTW_000732"
     CDS             19181..19525
                     /gene="erpA"
                     /locus_tag="ACHKTW_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HFO6461803.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(19572..20195)
                     /gene="yadS"
                     /locus_tag="ACHKTW_000733"
     CDS             complement(19572..20195)
                     /gene="yadS"
                     /locus_tag="ACHKTW_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HFO6461804.1"
                     /translation="MLVYWLDIVGTAVFAISGVLLAGKLRMDPFGVLVLGVVTAVGGG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLAREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(20233..21033)
                     /gene="btuF"
                     /locus_tag="ACHKTW_000734"
     CDS             complement(20233..21033)
                     /gene="btuF"
                     /locus_tag="ACHKTW_000734"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HFO6461805.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLQDQYAQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(21026..21724)
                     /gene="mtnN"
                     /locus_tag="ACHKTW_000735"
     CDS             complement(21026..21724)
                     /gene="mtnN"
                     /locus_tag="ACHKTW_000735"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFO6461806.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            21808..23325
                     /gene="dgt"
                     /locus_tag="ACHKTW_000736"
     CDS             21808..23325
                     /gene="dgt"
                     /locus_tag="ACHKTW_000736"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0006203 - dGTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HFO6461807.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAAL
                     RLRDGEEPLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRFPIESRLFHKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            23455..24879
                     /gene="degP"
                     /locus_tag="ACHKTW_000737"
     CDS             23455..24879
                     /gene="degP"
                     /locus_tag="ACHKTW_000737"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HFO6461808.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKNLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSSIYLLMQ"
     gene            25034..26191
                     /gene="cdaR"
                     /locus_tag="ACHKTW_000738"
     CDS             25034..26191
                     /gene="cdaR"
                     /locus_tag="ACHKTW_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
                     /protein_id="HFO6461809.1"
                     /translation="MAGWHLDTKMAQDIVARTMRIIDTNINVMDARGRIIGSGDRERI
                     GELHEGALLVLSQGRVVDIDDAVARHLHGVRQGINLPLRLEGEIVGVIGLTGEPENLR
                     KYGELVCMTAEMMLEQSRLMHLLAQDSRLREELVMNLIQAEENTPALTEWAQRLGIDL
                     NQPRVVAIVEVDSGQLGVDSAMAELQQLQNALTTPERNNLVAIVSLTEMVVLKPALNS
                     FGRWDAEDHRKRVEQLITRMKEYGQLRFRVSLGNYFTGPGSIARSYRTAKTTMVVGKQ
                     RMPESRCYFYQDLMLPVLLDSLRGDWQANELARPLARLKAMDNNGLLRRTLAAWFRHN
                     VQPLATSKALFIHRNTLEYRLNRISELTGLDLGNFDDRLLLYVALQLDEER"
     gene            complement(26280..26666)
                     /gene="yaeH"
                     /locus_tag="ACHKTW_000739"
     CDS             complement(26280..26666)
                     /gene="yaeH"
                     /locus_tag="ACHKTW_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HFO6461810.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(26981..27805)
                     /gene="dapD"
                     /locus_tag="ACHKTW_000740"
     CDS             complement(26981..27805)
                     /gene="dapD"
                     /locus_tag="ACHKTW_000740"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HFO6461811.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(27836..30508)
                     /gene="glnD"
                     /locus_tag="ACHKTW_000741"
     CDS             complement(27836..30508)
                     /gene="glnD"
                     /locus_tag="ACHKTW_000741"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HFO6461812.1"
                     /translation="MNTLPEQYANTALPTLPGQPQNPCVWPRDELTVGGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPIDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPEARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(30570..31364)
                     /gene="map"
                     /locus_tag="ACHKTW_000742"
     CDS             complement(30570..31364)
                     /gene="map"
                     /locus_tag="ACHKTW_000742"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFO6461813.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            31732..32457
                     /gene="rpsB"
                     /locus_tag="ACHKTW_000743"
     CDS             31732..32457
                     /gene="rpsB"
                     /locus_tag="ACHKTW_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFO6461814.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIASRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFEKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            32715..33566
                     /gene="tsf"
                     /locus_tag="ACHKTW_000744"
     CDS             32715..33566
                     /gene="tsf"
                     /locus_tag="ACHKTW_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFO6461815.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            33713..34438
                     /gene="pyrH"
                     /locus_tag="ACHKTW_000745"
     CDS             33713..34438
                     /gene="pyrH"
                     /locus_tag="ACHKTW_000745"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFO6461816.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            34730..35287
                     /gene="frr"
                     /locus_tag="ACHKTW_000746"
     CDS             34730..35287
                     /gene="frr"
                     /locus_tag="ACHKTW_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFO6461817.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            35379..36575
                     /gene="ispC"
                     /locus_tag="ACHKTW_000747"
     CDS             35379..36575
                     /gene="ispC"
                     /locus_tag="ACHKTW_000747"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HFO6461818.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEDVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYDRYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANAREVARKE
                     VMRLAS"
     gene            36764..37522
                     /gene="ispU"
                     /locus_tag="ACHKTW_000748"
                     /gene_synonym="uppS"
     CDS             36764..37522
                     /gene="ispU"
                     /locus_tag="ACHKTW_000748"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002094 - polyprenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008834 -
                     di-trans,poly-cis-undecaprenyl-diphosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0043164 - Gram-negative-bacterium-type cell
                     wall biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HFO6461819.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            37535..38392
                     /gene="cdsA"
                     /locus_tag="ACHKTW_000749"
     CDS             37535..38392
                     /gene="cdsA"
                     /locus_tag="ACHKTW_000749"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFO6461820.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            38404..39756
                     /gene="rseP"
                     /locus_tag="ACHKTW_000750"
     CDS             38404..39756
                     /gene="rseP"
                     /locus_tag="ACHKTW_000750"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HFO6461821.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            39786..42218
                     /gene="bamA"
                     /locus_tag="ACHKTW_000751"
     CDS             39786..42218
                     /gene="bamA"
                     /locus_tag="ACHKTW_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0071709 - membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HFO6461822.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            42340..42825
                     /gene="skp"
                     /locus_tag="ACHKTW_000752"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             42340..42825
                     /gene="skp"
                     /locus_tag="ACHKTW_000752"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HFO6461823.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            42829..43854
                     /gene="lpxD"
                     /locus_tag="ACHKTW_000753"
     CDS             42829..43854
                     /gene="lpxD"
                     /locus_tag="ACHKTW_000753"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HFO6461824.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            43959..44414
                     /gene="fabZ"
                     /locus_tag="ACHKTW_000754"
     CDS             43959..44414
                     /gene="fabZ"
                     /locus_tag="ACHKTW_000754"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFO6461825.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            44418..45206
                     /gene="lpxA"
                     /locus_tag="ACHKTW_000755"
     CDS             44418..45206
                     /gene="lpxA"
                     /locus_tag="ACHKTW_000755"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HFO6461826.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            45206..46354
                     /gene="lpxB"
                     /locus_tag="ACHKTW_000756"
     CDS             45206..46354
                     /gene="lpxB"
                     /locus_tag="ACHKTW_000756"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HFO6461827.1"
                     /translation="MTEQRPLTIALVAGETSGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDIFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDMVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNAARDVLGIPHDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            46351..46947
                     /gene="rnhB"
                     /locus_tag="ACHKTW_000757"
     CDS             46351..46947
                     /gene="rnhB"
                     /locus_tag="ACHKTW_000757"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFO6461828.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALYEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEHGATEHHRRSFGPVKRALGLAS"
     gene            46984..50466
                     /gene="dnaE"
                     /locus_tag="ACHKTW_000758"
     CDS             46984..50466
                     /gene="dnaE"
                     /locus_tag="ACHKTW_000758"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFO6461829.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLTLLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERVKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKPVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            50479..51438
                     /gene="accA"
                     /locus_tag="ACHKTW_000759"
     CDS             50479..51438
                     /gene="accA"
                     /locus_tag="ACHKTW_000759"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HFO6461830.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            51537..53678
                     /gene="ldcC"
                     /locus_tag="ACHKTW_000760"
     CDS             51537..53678
                     /gene="ldcC"
                     /locus_tag="ACHKTW_000760"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HFO6461831.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTDEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GTSTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVIFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVETAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECWPVAPGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLPGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            53735..54124
                     /gene="yaeR"
                     /locus_tag="ACHKTW_000761"
     CDS             53735..54124
                     /gene="yaeR"
                     /locus_tag="ACHKTW_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFO6461832.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDTLGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            54189..55487
                     /gene="tilS"
                     /locus_tag="ACHKTW_000762"
     CDS             54189..55487
                     /gene="tilS"
                     /locus_tag="ACHKTW_000762"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFO6461833.1"
                     /translation="MTLTLNRQLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVTHCENVCQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMGEVSEFAGTRLIRPLLARTR
                     GELAQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSAALCAE
                     QESLLDELLADDLAHCQTSQGTLQIAPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRFGAFEIRRYQSQLWWINSVTGQSETIVPWQTWLQPLELPA
                     GLGSVQLTAGGDIRPPRADEAVSVRFKAPGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGVSFVWQKTLS"
     gene            complement(55536..55796)
                     /gene="rof"
                     /locus_tag="ACHKTW_000763"
     CDS             complement(55536..55796)
                     /gene="rof"
                     /locus_tag="ACHKTW_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HFO6461834.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(55783..55983)
                     /gene="yaeP"
                     /locus_tag="ACHKTW_000764"
     CDS             complement(55783..55983)
                     /gene="yaeP"
                     /locus_tag="ACHKTW_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HFO6461835.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            56149..56694
                     /gene="yaeQ"
                     /locus_tag="ACHKTW_000765"
     CDS             56149..56694
                     /gene="yaeQ"
                     /locus_tag="ACHKTW_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HFO6461836.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     VLFTYNSRAAQIWWQQNQSKCVQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            56691..57113
                     /gene="arfB"
                     /locus_tag="ACHKTW_000766"
     CDS             56691..57113
                     /gene="arfB"
                     /locus_tag="ACHKTW_000766"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HFO6461837.1"
                     /translation="MIVISRHVAIPDGELEITAIRAQGVGGQHVNKTSTAIHLRFDIR
                     ASSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKDLTTEQ
                     KARRPTRPTRASKERRLASKAQKSSVKAMRGKVRSGRE"
     gene            57127..57837
                     /gene="nlpE"
                     /locus_tag="ACHKTW_000767"
                     /gene_synonym="cutF"
     CDS             57127..57837
                     /gene="nlpE"
                     /locus_tag="ACHKTW_000767"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308221.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HFO6461838.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDALSPAQAAELKPMPQS
                     WRGVLPCADCEGIETSLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERGYLAARGHSEKPMLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNQDCSSLGQ"
     gene            complement(57992..58815)
                     /gene="yaeF"
                     /locus_tag="ACHKTW_000768"
                     /pseudo
     CDS             complement(57992..58815)
                     /gene="yaeF"
                     /locus_tag="ACHKTW_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414735.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
     gene            complement(58869..60587)
                     /gene="proS"
                     /locus_tag="ACHKTW_000769"
     CDS             complement(58869..60587)
                     /gene="proS"
                     /locus_tag="ACHKTW_000769"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFO6461839.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(60699..61406)
                     /gene="tsaA"
                     /locus_tag="ACHKTW_000770"
     CDS             complement(60699..61406)
                     /gene="tsaA"
                     /locus_tag="ACHKTW_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HFO6461840.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIAPYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKDSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(61403..61807)
                     /gene="rcsF"
                     /locus_tag="ACHKTW_000771"
     CDS             complement(61403..61807)
                     /gene="rcsF"
                     /locus_tag="ACHKTW_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HFO6461841.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKRMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
     gene            complement(61925..62740)
                     /gene="metQ"
                     /locus_tag="ACHKTW_000772"
     CDS             complement(61925..62740)
                     /gene="metQ"
                     /locus_tag="ACHKTW_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HFO6461842.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(62780..63433)
                     /gene="metI"
                     /locus_tag="ACHKTW_000773"
     CDS             complement(62780..63433)
                     /gene="metI"
                     /locus_tag="ACHKTW_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HFO6461843.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVRKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(63426..64457)
                     /gene="metN"
                     /locus_tag="ACHKTW_000774"
     CDS             complement(63426..64457)
                     /gene="metN"
                     /locus_tag="ACHKTW_000774"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HFO6461844.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQAEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            64645..65220
                     /gene="gmhB"
                     /locus_tag="ACHKTW_000775"
     CDS             64645..65220
                     /gene="gmhB"
                     /locus_tag="ACHKTW_000775"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HFO6461845.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAVAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPAQ"
BASE COUNT        15420 a        16314 c        18057 g        15637 t
ORIGIN      
        1 ctccccatca ggaaacgcca gctccggtgc aatctcaaac agcggccaca gcataaatcc
       61 acgattcttc atatcgtagt gcggaacggt caggcgttcg gtatttatca cttcattacc
      121 aaacagcatg atatcgagat ccagcgtgcg tggtccccag cgttcagctt tacgaacacg
      181 accctgttgc agttctatgc gctgtgtatg gttgagtaac tcttccgggg cgagcgaagt
      241 ttcaagcgcc acggcggcgt tgaggtaatc gggttgatct tgcggcccca gcggtggggt
      301 gcggtaaaac gaagaaacgg caagaatgtg gctttcaggg atatcgccta atgctttcag
      361 ggcagcattg acctgctcca gcggagaggc cagattgctg cctatggcaa tatacgccac
      421 tgtcatgcgg taccctcacg acgcggtgcg cgtttgcgtg gacgacgagt acggcgacgc
      481 ggtgacggct cttcatccaa ctcgttaagc atcccttttt gatctggtgg cgcggaaacc
      541 tggaactcac cccaccattt caccagacgc tgcagttcag cgttacgttc aacttcagct
      601 cgcaaggcca acaggtcata agccgcacgg aacttaggat gctccagcag tttccatgcg
      661 cgtttaccct gacgacggga catacgcaac tgcaactgcc agatatcgcg ggttaacgtc
      721 gtcagacgtt tcgggattgc cagtgaacgg caggcttcgt ccagcacgtc gttcatcgcc
      781 agcgcgaaag cgtcgtgata ggtcaggccg ctttcctggg caatcttctg tgccgtctcc
      841 agcagtgggt accagaacat ggcggcaaac aggaacgccg ggttcacgcg catatcgtta
      901 tggatacgcg tatcggtatt cttcagcacc tgttcaatga tccgctccat cgggctgtcg
      961 ccattttccg tgaagtagcg ggtaatggtc gggaacagcg gctggaacag atgatattca
     1021 cacaacagct tataggtatc gtaaccgtag cccgcttgta gcagtttaag cgattcttca
     1081 aacaggcgtg ccggtgggat atcgttcaac agggtagcga ggcgagggat cggttctgcg
     1141 gtttccgggc tgatgcgcat acccaatttg gcggcaaaac gtaccgcgcg cagcatacgt
     1201 accggatctt cacggtagcg cgtttccggg ttaccaatca gacggataac gccgtccttc
     1261 agatccttca taccgccaac gtaatcacgg acggtaaaat ccgctacgct gtaatagagg
     1321 ctgttgatag tgaaatcgcg gcgctgggcg tcttcttcga tggagccgaa aatgttgtcg
     1381 cgcagcaaca tgccgttttg cccgcgttgg gaggtcgtgc ggtcgctgac gttaccttcg
     1441 tggtgtccac ggaaggtcgc aacttcgata atctccgggc caaacattac atgagccaga
     1501 cggaaacggc gacccaccag gcggcagtta cggaacagtt tgcgcacctg ctcaggcgtg
     1561 gcgttagtgg ttacgtcaaa atctttcggc tttttgccaa gtaacaggtc gcgcacgccg
     1621 ccgccaacca gccaggcttc gtatcccgct ttgttgagcc tgtacattac cttcagggca
     1681 ttttcactga tatctttgcg ggaaatagca tgctgctcac gcgggatcac cgtcacctgt
     1741 ggacgggcga ctgcctgttc agcctcgctt tcctcgcggc ttagcacctt gcggcaaaaa
     1801 ttagcgactc gggtaaaaat agtacacctc ggtagtgtca aacatcattc aggacaaaaa
     1861 aatagcggct aatcatagct cagcatgacg catttgagaa tgttgaattt acaattgccg
     1921 actcgggcac ggcggtaagc cgccagtttt tgacggctga ctgaaggatt tgctcgacgc
     1981 tgaaatcctg ccagtgtgct tctgcctgct gccccagaaa ttgaagtgcc gcgattagta
     2041 ccgggcgtgg atcgcctttc ggcaacgcag gcgcatgatt ctgcttggaa agtttagcgc
     2101 cttgtggatt aagcgccagc ggcagatgaa tgtaatctgg cactttccag ccaaaaagct
     2161 ggtacagcga gatttgcctt actgttggtt caatcagatc agccccacgc actatttctg
     2221 taacgccctg gaaatgatca tcaaccacaa cagccaggtt gtaggcgaac aacccatcac
     2281 ggcgatgaat gataaaatct tcccgtgcca gtttttcgtc ggcgtgaata atgccgcgca
     2341 gctggtcagt aaattgcgtg accggatgct gctggcggat acgcactgcg gcattgtctg
     2401 gtccatgatg caacacccgg caatgaccgt cgtaaatacc gccaatgctt tgaatacgcg
     2461 cacgcgtaca ggtgcagtaa taacttagtc cttgttcatg taaccaggcg agtgcttcac
     2521 gataggcgtg gtgacgttgc gattgccaga gaacatcgcc gtcccagtgc agaccgtaat
     2581 gttccagctg gcgcaggata gtttctgcgg caccgggaac ttcacgaggc gggtcgatat
     2641 cttctatgcg taccagccag cgaccttgcc gggcgcgagc ctgcaaatag ctgccgagcg
     2701 cggcgatcag agagccaaaa tgaagctcgc cggaaggaga gggggcgaag cggccaatat
     2761 actgtgtgtc tgtcatctct ttgaacaaaa aataaggcgg gagcatttcc cgcctgtggt
     2821 aaacgtgatg gaacggctgt aattagccag ccatctgttt ttcgcgaatt tcagccagcg
     2881 ttttgcagtc gatgcacaga tcggctgtcg ggcgcgcttc cagacggcga ataccaattt
     2941 caacaccgca ggattcgcag tagccgaaat cttcgtcttc cacttttttc agcgtcttct
     3001 cgatcttttt gatcagctta cgctcgcgat cgcggttacg cagttcgagg ctgaactctt
     3061 cttcctgggc tgcacggtct accgggtccg ggaagttggc tgcttcatcc tgcatatgtg
     3121 taacggtgcg atcgacttca tccctgagtt gattacgcca tgcttccaga atacgacgga
     3181 agtgcgccag ctgggcttca ttcatatact cttcgcccgg cttctcctga tatggttcca
     3241 ccccagcgat ggcgagaata ctcagggacg atgttttacg gttttgccct tcttgcatgt
     3301 tgcttctcct taacacgcac tatcgatccc catgttcggg ggaaaaatga ggccgctata
     3361 aatagcagat gcttttccgg atagcaatta tctaaacgta acacttgaca actgtgtgag
     3421 gaaaagcgta tttgcgcacg cgaccagaat gtaaattaac cagttactta ctttactaca
     3481 atgtaaccgg cagtgatttt ttaagagcca tgccttcagc agaaatttcc gctttgtaag
     3541 ccagaatttc tacccccctc tgttgagctt ctgacaatag ttgcgcgtat ttctcatcga
     3601 tgtggcgcgc gggtgaaaac cgtgtaatgg ctgaatgcag cacggcgaaa aagataaccg
     3661 cacgctggcc ttcagccgct acgctcatca actcccgaag gtgtttctga cctcgttcag
     3721 tgaccgcatc gggaaaatat ccctgttcgt tctccgctaa cgtaaccgat ttcacttcaa
     3781 tatagcagtc tggacgcgaa tccgcctgca acataaagtc aatacggctg agttctgcgc
     3841 cgtattttac ttcgcttttc agcgagctat agcctgacag ttctgaaatt gattcattaa
     3901 ggatagcctc tttcgtcaac ctgttagccc aaagcgtgtt gacgcaaata aatgcgccgc
     3961 tctggctttg agttaattcc caggtgtgtg ggtatttccg tttggtgttg tctgaagtcg
     4021 aataccagac ggtatcgcca ggcgttgcac aaccggtcat tgcacccgta ttcgggcagt
     4081 gtagcgttaa ttcgcgacca tcgggtgtga tcacatcggc taaaaaacgt ttgtaacgct
     4141 gaattagcgt cgcgcgctgt agagggggag agaattccat tgcgacaatc cttgttattg
     4201 cgttagcgcc cagcgtttta gcggcgtgta gcgtgtgcgt ccacgggcaa acgaggaggc
     4261 gtaaagggtg aactccgtca ccgcataaga ccagttaaaa cctggcggcg ggattgtcac
     4321 cgcctcgctg gcgtcgcgca ataaggtaat atgtggatga aacggacgat tgctttgaaa
     4381 acaaccgctg cgggcagcct gtgaacggag catattcgcc agctggatta agccgcgtgg
     4441 aggctgacgc atccctaacc acaccacacg cgaacgcagc cattgtccgg cgtcatcaag
     4501 cgtgagtgtg aaaccaggtt gacgaatccg tccggctaaa agagaaagcg ccttctcttt
     4561 ctcggcgctc acttcgccta aaaatgccag agtcagatgc aaattatcgg cggcgaccgg
     4621 acgtcccgcc tcaggtggga agtgtgtggc gcgccaatgg ataatctgtt cgcggatttc
     4681 tgcaggtaag tcgatagcaa agaacagacg ttgcggttca gacatgtgag gcactcggtt
     4741 atgaattacc gcgatgctac aatgtggcgc gaagaatgtt aaccctctgg agcgttttgt
     4801 gtcgtcgttg cccgttgctg ccgtcttatc tgaattactt accgctctcg attgtgcgcc
     4861 gcaggtatta ttaagtgcgc cgaccggggc cgggaaatca acctggctgc cgctgcaact
     4921 gctggcgcat cccggcatta acgggaaaat tatcctgctg gagccgcgtc gtctggcggc
     4981 gcgtaacgtc gcgcaacggc tggcggagct gcttaacgaa aagccaggcg ataccgttgg
     5041 ctaccggatg cgtgcgcaaa actgcgtcgg gccgaatacc cgcctggaag tggttaccga
     5101 aggcgtgctg acgcgcatga tccagcgtga cccggaactg agcggtgttg gactggtgat
     5161 ccttgatgaa tttcatgagc gcagcttgca ggcggatttg gcgttggcgc tgttactcga
     5221 tgtgcaacaa ggtctgcgtg atgaccttaa actgctgatt atgtcggcta ccctggacaa
     5281 cgaccgcttg cagcaaatgc tgccagaagc gcctgtcgtc atctcagaag ggcgctcgtt
     5341 tccggttgaa cgccgttatt taccgctgcc cgcgcatcag cgttttgacg atgccgttgc
     5401 ggtagccacc gctgaaatgc tgcgtcagga aagcggatca ttactgttat ttttacctgg
     5461 cgtcggagaa attcagcgtg tgcaggaaca actggcttcg cgcatcggca gtgatgtatt
     5521 gctctgcccg ctgtatggcg cgttgtcgct gaacgatcag cgaaaagcga tcctcccggc
     5581 accgcaaggg atgcgcaaag tggtgctggc gaccaatatt gctgaaacca gtttaaccat
     5641 tgaaggtatt cgtctggtgg tggattgtgc ccaggagcgt gtggcgcgtt ttgatccgcg
     5701 cacggggctt acgcgactga ttactcaacg cgttagccag gcatccatga cgcagcgtgc
     5761 cgggcgcgcc gggcgtctgg agccgggtat ctgcctgcat ttaatcgcca aagaacaagc
     5821 agaacgcgcc gcggcgcaaa gtgaaccgga gatcttacaa agcgatcttt ccggtttgct
     5881 gatggaatta ctgcaatggg gatgcagcga tccggcgcag atgagctggc tggatcaacc
     5941 gccagtagtg aatctactgg ccgcgaaacg tctgttacaa atgctggggg cactggaggg
     6001 tgaacggctt agtgcgcaag ggcaaaaaat ggcagcgctg ggtaacgatc cgcgtttagc
     6061 ggcaatgctg gttagcgcga agaacgacga cgaagctgct accgcggcaa aaattgccgc
     6121 cattctcgaa gagccgccac ggatgggcaa tagtgacctg ggcgtggcgt tttcgcgcaa
     6181 tcaaccagcc tggcagcaac gtagtcagca actgttaaaa cgcttaaacg tacgtggcgg
     6241 tgaggcagac agttcgctta tcgcgccgct acttgccggg gcgtttgccg atcgcattgc
     6301 tcgtcgccgt gggcaagatg gacgctatca actggcaaac ggcatgggag cgatgctcga
     6361 tgccaacgac gcgctaagcc gccacgaatg gttgatcgca ccgttattat tgcagggcag
     6421 cgcctcgccg gatgcgcgga ttttactggc gctgccggtc gatattgatg agttagtaca
     6481 acgctgcccg cagctggtac agcagtctga cactgtggag tgggatgacg cgcaaggtac
     6541 gctgaaagcc tggcgtcggc tacaaatcgg tcagttgacg gtgaaagtgc agccgctggc
     6601 gaaaccgtca gaagacgagt tgcatcaggc gatgcttaat ggcatccgtg ataaaggttt
     6661 aagcgtgctc aactggacgg cggaagcgga acagctacgc ttgcgtttgt tatgcgccgc
     6721 aaagtggttg ccggaatatg actggccagc ggttgatgat gaaagtttat tggcagcgct
     6781 ggaaacgtgg ctgctgccac atatgactgg cgtacattca ctacgcggcc tgaaatcact
     6841 cgacatttat caggcactac gcggattact tgattgggga atgcagcaac gtctggatag
     6901 tgaattgcct gcgcattaca ctgtgccgac gggaagccgg atcgccattc gttatcatga
     6961 agataacctg cccgcgctgg cggtgagaat gcaagagatg tttggcgagg ccaccaatcc
     7021 gacgatcgcc caggggcgcg tgccgctggt gctggagttg ctttcacctg cccaaaggcc
     7081 attacaaatc acacgagatt tgagcgcctt ctggaaagga gcgtaccgtg aggtgcaaaa
     7141 agagatgaaa gggcgttatc ccaaacatgt ctggccggac gacccggcaa atactgcacc
     7201 gacgcgacgg acgaaaaagt attcgtaagg ttggttttct ccctctccct gtgggagagg
     7261 gccggggtga gggcatcagc gcgcacgttc accctcaccc taaccctctc cctcaaggga
     7321 gaggggaccg atcgagcaca aattttgaga gatatcttct tctgtcttgt aacagaagaa
     7381 cagaaaatcg ggcttttgcg cctgaatatt gcggagaaaa agcatggccg ggaatgaccg
     7441 cgagccaatt ggacgcaaag ggaaaccgac gcgtccggtc aaacaaaagg taagccgtcg
     7501 tcgttacgaa gatgacgatg attacgacga ttatgatgac tatgaggatg aagaaccgat
     7561 gccgcgcaaa ggtaagggca aaggcaaagg gcgtaagcct cgtggcaaac gcggctggct
     7621 atggctactg ctaaaactgg ctatcgtttt tgccgtgctg atcgccattt acggcgttta
     7681 tctcgatcaa aaaattcgta gccgtattga tggcaaggtc tggcaactgc ctgcggcagt
     7741 ttatggccga atggtcaatc ttgagccaga catgaccatc agcaagaacg agatggtgaa
     7801 gctgctggag gcgacccagt atcgtcaggt gtcgaaaatg acccgtcctg gcgaatttac
     7861 cgtgcaggcc aacagcattg agatgattcg ccgtccgttt gatttcccgg acagtaaaga
     7921 aggacaggtg cgcgcgcgtc tgacctttga tggcgatcat ctggcgacga tcgtcaatat
     7981 ggagaacaac cgtcagttcg gtttcttccg tcttgatccg cgtctgatca ccatgatctc
     8041 ttcgccaaac ggtgagcagc gtctgtttgt gccgcgcagt ggtttcccgg atttgctggt
     8101 ggatactttg ctggcgacag aagaccgtca tttttacgag catgatggaa tcagtctcta
     8161 ctcaatcgga cgtgcggtgc tggcaaacct gaccgccgga cgcacggtac agggtgcgag
     8221 tacgctgacg caacagctgg tgaaaaacct gttcctctcc agcgagcgtt cttactggcg
     8281 taaagcgaac gaagcttaca tggcgctgat catggacgcg cgttacagca aagaccgtat
     8341 tcttgagctg tatatgaacg aggtgtatct cggtcagagc ggcgacaacg aaatccgcgg
     8401 cttcccgctg gcaagcttgt attactttgg tcgcccggta gaagagctaa gcctcgacca
     8461 gcaggcgctg ttagtcggta tggtgaaagg ggcgtccatc tacaacccgt ggcgtaaccc
     8521 aaaactggcg ctggagcgac gtaatctggt gctgcgtctg ctgcaacagc aacagattat
     8581 tgatcaagaa ctctatgaca tgttgagtgc ccgtccgctg ggggttcagc cgcgcggtgg
     8641 ggtgatctct cctcagccag cctttatgca actggtgcgt caggagctgc aggcaaaact
     8701 gggcgataag gtaaaagatc tctccggcgt gaagatcttc actacctttg actcggtggc
     8761 ccaggacgcg gcagaaaaag ccgccgtgga aggcattccg gcactgaaga aacagcgtaa
     8821 gttgagcgat cttgaaactg cgattgtggt cgtcgaccgc tttagtggtg aagttcgtgc
     8881 gatggtcgga ggttctgagc cgcagtttgc gggctacaac cgtgcgatgc aggcgcgtcg
     8941 ttcgattggt tcccttgcaa aaccagcgac ttatctgacg gctttaagcc agccgaaaat
     9001 ctatcgcctg aacacgtgga ttgccgatgc gccaatcgcg ctgcgtcagc cgaatggcca
     9061 ggtctggtca ccgcagaacg atgacagtcg ttatagcgaa agcggcagag tgatgctggt
     9121 ggatgcgttg acccgttcga tgaacgtgcc gacggtaaat ctggggatgg cgctggggct
     9181 gcctgcagtt acggatacct ggatcaaact gggcgtaccg aaagatcagc tgcatccggt
     9241 tccggcgatg ctgctggggg cgttgaactt aacgccaatc gaagtggcgc aggcgttcca
     9301 gaccatcgcc agcggtggta accgtgcacc gctttctgca ctgcgttcgg taatcgcgga
     9361 agatggcaag gtgctgtatc agagcttccc gcaggcagaa cgcgctgttc cggcgcaggc
     9421 ggcgtatctg acactatgga ccatgcagca ggtggtacaa cgtggtacgg gtcgtcagct
     9481 tggggcgaaa tacccgaacc tgcatctggc agggaaaaca gggactacca acaataacgt
     9541 agatacctgg tttgcaggta ttgacggcag cacggtgacc atcacctggg taggccgtga
     9601 taacaaccag ccgaccaaac tgtatggagc cagcggggca atgtcgattt atcagcgtta
     9661 tctggctaac cagacgccaa cgccgctgaa tcttgttccg ccagaagata ttgcagatat
     9721 gggcgtggac tacgacggca actttgtttg cagcggtggc atgcgtgtct tgccggtctg
     9781 gaccagcgat ccgcaatcgc tgtgccagca gagcgagatg cagcagcagc cgtcaggcaa
     9841 tccgtttgat cagtcttctc agccgcagca acagccgcaa cagcaacctg ctcagcaaga
     9901 gcagaaagac agcgacggtg tagccggttg gatcaaggat atgtttggta gtaattaaca
     9961 tctaagcgtg aaataccgga tggcgagttg ccatccggta aaataacatc ccatctaaga
    10021 tattaaccct ttcttttcat ctggttgttt attaaccctt caggaacgct cagattgcgt
    10081 accgcttgcg aacccgccag cgtttcgaat attatcttat ctttatgata atcattctcg
    10141 tttacgttat cattcacttt acatcagaga tataccaatg gcgcgttcca aaactgctca
    10201 gccaaaacac tcactgcgta aaatcgcagt tgtagtagcc acagcggtaa gcggcatgtc
    10261 tgtttatgca caggcagcgg ttgaaccgaa agaagacact atcaccgtta ccgctgcacc
    10321 tgcgccgcaa gaaagcgcat gggggcctgc tgcaactatt gcggcgcgac agtctgctac
    10381 cggcactaaa accgatacgc cgattcaaaa agtgccacag tctatttctg ttgtgaccgc
    10441 cgaagagatg gcgctgcatc agccgaagtc ggtaaaagaa gcgcttagct acacgccggg
    10501 tgtctctgtt ggtacgcgtg gcgcatccaa cacctatgac cacctgatca ttcgcggctt
    10561 tgcggcagaa ggccaaagcc agaataacta tctgaatggc ctgaagttgc agggcaactt
    10621 ctataacgat gcggtcattg acccgtatat gctggaacgc gctgaaatta tgcgtggccc
    10681 ggtttccgtg ctttacggta aaagcagtcc tggcggcctg ttgaatatgg tcagcaagcg
    10741 tccgaccacc gaaccgctga aagaagttca gtttaaagcc ggtactgaca gcctgttcca
    10801 gactggtttt gactttagcg attcgttgga tgatgacggt gtttactctt atcgcctgac
    10861 cggtcttgcg cgttctgcca atgcccagca gaaagggtca gaagagcagc gttatgctat
    10921 tgcaccggcg ttcacctggc gtccggatga taaaaccaat tttaccttcc tttcttactt
    10981 ccagaacgag ccggaaaccg gttattacgg ctggttgccg aaagagggaa ccgttgagcc
    11041 gctgccgaac ggtaagcgtc tgccgacaga ctttaatgaa ggggcgaaga acaacaccta
    11101 ttctcgtaat gagaagatgg tcggctacag cttcgatcac gaatttaacg acacctttac
    11161 tgtgcgtcag aacctgcgct ttgctgaaaa caaaacctcg caaaacagcg tttatggtta
    11221 cggcgtctgc tccgatccgg cgaatgctta cagcaaacag tgtgcggcat tagcgccagc
    11281 ggataaaggc cattatctgg cacgtaaata cgtcgttgat gatgagaagc tgcaaaactt
    11341 ctccgttgat acccagttgc agagcaagtt tgccactggc gatatcgacc acaccctgct
    11401 gaccggtgtc gactttatgc gtatgcgtaa tgacatcaac gcttggtttg gttacgacga
    11461 ctctgtgcca ctgctcaatc tgtacaatcc ggtgaatacc gatttcgact tcaatgccaa
    11521 agatccggca aactccggcc cttaccgcat tctgaataaa cagaaacaaa cgggcgttta
    11581 tgttcaggat caggcgcagt gggataaagt gctggtcacc ctgggcggtc gttatgactg
    11641 ggcagatcaa gaatctctta accgcgttgc tgggacgacc gataaacgtg atgacaaaca
    11701 gtttacctgg cgtggtggtg ttaactacct gtttgataat ggtgtaacac cttacttcag
    11761 ctatagcgaa tcgtttgaac cttcttcgca agttgggaag gatggtaata ttttcgcacc
    11821 gtctaaaggt aagcagtatg aagtcggcgt gaaatatgta ccggaagatc gtccgattgt
    11881 agttactggt gccgtgtata atctcactaa aaccaacaac ctgatggcgg accctgaggg
    11941 ttccttcttc tcggttgaag gtggcgagat ccgcgcacgt ggcgtagaaa tcgaagcgaa
    12001 agcggcgctg tcggcgagtg ttaacgtagt cggttcttat acttacaccg atgcggaata
    12061 caccaccgat actacctata aaggcaatac gcctgcacag gtgccaaaac acatggcttc
    12121 gctgtgggct gactatacct tctttgacgg tccgctttca ggtctgacgc tgggcaccgg
    12181 tggtcgttat actggctcca gctatggtga tccggctaac tcctttaaag tgggaagtta
    12241 tacggtcgtg gatgcgttag tgcgttatga tctggcgcga gtcggcatgg cgggctccaa
    12301 cgtggcgctg catgttaaca acctgttcga tcgtgaatac gtcgccagct gctttaacac
    12361 ttatggctgc ttctggggcg cagaacgtca ggtcgttgca accgcaacct tccgtttcta
    12421 atttctcttt tggggcacgg atttccgtgc ccatttcaca agttggctgt tatgcaggaa
    12481 tacacgaatc attccgatac cacttttgca ctgcgtaata tctcctttcg tgtgcccggg
    12541 cgcacgcttt tgcatccgct gtcgttaacc tttcctgccg ggaaagtgac cggtctgatt
    12601 ggtcacaacg gttctggtaa atccactctg ctcaaaatgc ttggccgtca tcagcctccg
    12661 tcagaagggg agattcttct tgatgcccag ccgctggaaa gctggagcag caaagcgttt
    12721 gcccgcaaag tggcttattt gccgcagcag cttcctccgg cagaagggat gaccgtgcgt
    12781 gaactggtgg cgattggtcg ttacccgtgg catggcgcgc tggggcgctt tggtgcggca
    12841 gatcgcgaaa aggtcgagga agctatctcg ctggttggct taaaaccgct ggcgcatcgg
    12901 ctggtcgata gtctctctgg cggcgaacgt cagcgggcgt ggatcgccat gctggtggcg
    12961 caggatagcc gttgtctgtt gctcgacgaa ccgacttcgg cgctggatat cgcccaccag
    13021 gttgatgtgc tggcgctggt gcaccgttta agtcaggaac gtggcctgac ggtcattgcc
    13081 gtgctgcacg atatcaatat ggcggcacgc tactgtgatt atctggtcgc cctgcgcggt
    13141 ggtgaaatga ttgctcaggg aacgcctgcg gaaattatgc gcggcgaaac cctcgaaatg
    13201 atttatggca tcccgatggg tattttgccg catccggcgg gtgctgcacc tgtgagtttt
    13261 gtttattgat gagcggctta cctcttattt cgcgccgtcg actgttaacg gcgatggcgc
    13321 tttctccgtt gttatggcag atgaataccg cccacgcggc ggctattgac cccaatcgta
    13381 ttgtggcgct ggagtggttg ccggtggaat tactgctggc gctcggcatc gtgccttacg
    13441 gcgtggcgga taccatcaac tatcgcctgt gggtcagcga accgctattg ccggactcag
    13501 tgattgacgt tggtttgcgt acagaaccta accttgaact gctgaccgaa atgaaaccgt
    13561 cgtttatggt ctggtcggca ggatatggcc cttcaccaga aatgctggca cgtattgcac
    13621 cgggtcgcgg atttaacttc agcgacggca aacagccgct ggcgatggcg cgtaaatcgc
    13681 tgacggaaat ggcagattta cttaacctgc aaagcgcagc agaaacgcat ttagcgcaat
    13741 atgaagactt tatccgcagc atgaaacccc gctttgtgaa gcgtggtgca cgcccgttat
    13801 tgctgacgac gcttatcgat ccgcgccata tgctggtttt cggtccaaac agcttgtttc
    13861 aggaaattct cgatgagtac ggcatcccaa atgcctggca aggggaaacc aacttctggg
    13921 gcagtaccgc cgtcagtatc gatcgtctgg cggcgtataa agacgttgat gtgctctgtt
    13981 ttgatcacga caacagcaaa gacatggatg cgctaatggc aacgccgctg tggcaggcca
    14041 tgccgtttgt ccgcgccgga cgctttcagc gcgtacctgc agtctggttt tatggtgcga
    14101 cgctctcggc aatgcacttt gtgcgcgttc tggataacgc catcggaggt aaagcgtgag
    14161 taaacgaatt gcgctttttc cggcgttatt gctggcgctg ttggtgattg ccgctacggc
    14221 gctcacctgg atgaattttt cgcaggcgct gccgcgcagc cagtgggcgc aggccacctg
    14281 gtcgccggat attgacgtca tcgagcagat gatttttcac tacagcttgt tgccgcgtct
    14341 ggcgatttcg ctgctggtgg gcgcgggcct ggggctggtg ggcgtgctgt ttcagcaagt
    14401 gctgcgtaac ccgctggcgg agccgacgac gcttggcgtt gctacaggcg cgcaactggg
    14461 aattaccgtc accacacttt gggcgatccc aggtgctatg gcgagccagt ttgccgcgct
    14521 ggtgggcgct tgtgtcgttg gcttaatcgt ctttggcgtc gcgtggggga aacggctttc
    14581 gccggttacg cttattctcg cggggctggt ggtgagcctt tattgcgggg caattaatca
    14641 gctactggtt atcttccatc acgaccagct gcaaagcatg ttcctgtgga gcaccggaac
    14701 gctgacgcaa accgactggg gcggcgttga gcgtttatgg ccgcagttgc tgggcggcgt
    14761 gatgttgacg ttattgctac ttcgcccgtt aaccctgatg gggcttgatg atggcgtggc
    14821 gcgcaatctc gggctggcct tgtcgctcgc gcgtctggca gcgctgtcgc tggcgattgt
    14881 catcagtgcg ctgctggtga acgctgtggg gattatcggc tttatcgggt tgttcgcgcc
    14941 gctgctggca aaaatgctgg gggcgcggcg tctgctgcca cgactgatgc tggcgtcgct
    15001 gattggggcg ctgatcctct ggctttccga tcaaatcatc ctctggctga ctcgcgtgtg
    15061 gatggaagtg tccaccggtt cggtcactgc gttgatcggt gcgccgctgc tactgtggct
    15121 gttgccgcgt ttacgcagca ttagcgcgcc ggatatgaag gtcaacgatc gtgtcgcggc
    15181 tgaacgccaa catgtgctgg cgtttgccct cgcgggcggc gtgctgctgt tgatggctgt
    15241 ggtggtggcg ctgtcgtttg gtcgtgatgc gcacggctgg acgtgggcga gcggggcgtt
    15301 gcttgaggat ttgatgccct ggcgctggcc gcgaattatg gcggcgctgt ttgcgggcgt
    15361 catgctggcg gtggcgggct gtattattca gcggctgacc ggaaacccga tggcaagccc
    15421 ggaagtgctg gggattagct ctggcgcagc gtttggcgtg gtgttgatgc tgtttctggt
    15481 gccgggtaat gcctttggct ggctgttacc tgcaggcagt ctcggcgcgg cggtgacgct
    15541 gttgatcatt atgatcgccg ccggccgcgg tggattttcc ccacaccgca tgttactggc
    15601 ggggatggcg ttaagcaccg cgttcaccat gcttttgatg atgttgcagg caagtggtga
    15661 cccgcgaatg gcgcaagtgc tgacctggat ttccggttcg acctacaacg cgaccgatgc
    15721 gcaggtctgg cgcacgggaa ttgtgatggt gattttgctg gcgattaccc cgctgtgccg
    15781 ccgctggctg accattttac cgctgggtgg tgataccgcc cgagccgtag gaatggcgct
    15841 gacgccgacg cgaattgcgc tgctgctgtt agcggcttgc ctgacggcga ccgcgacgat
    15901 gactattgga ccgttgagtt ttgttggttt aatggcaccg catattgcgc ggatgatggg
    15961 ctttcgacgg acgatgccac acatcgtaat ttcggcgctg gtgggtggtt tactgctggt
    16021 gttcgctgac tggtgtgggc ggatggtgct gtttccattc cagatcccgg cagggctgct
    16081 gtcgaccttt atcggcgcgc catattttat ctatttgttg agaaagcaga tccgttaata
    16141 gaatgcctcg cccggtgggc gaggcaaaat catcacaact tcgcaaacac ccgtcgtgca
    16201 gcatcgatgg tgttattgat atcttccatg ctgtgcgcca cggacataaa gcccgcttca
    16261 aacgctgacg gtgccaggta aacaccttcg tccagcatca tatggaagaa acgcttaaag
    16321 cgttccacgt cacaggccat cacatcctga tagcacgtca cggactcggc gtcggtaaag
    16381 aaaataccga acatgccgcc aacgtggtta acgaccagcg gaattccggc ttcttctgcc
    16441 gcttccagca gaccttctgc cagacgtgat gtcagctcat ccaacgtttc gtgaacgccc
    16501 ggctgcgcga cttcattcag acaggcgaaa cccgctgcca tcgcaattgg gttaccggaa
    16561 agcgtacccg cctgatagac cggacccgtc ggggccagcg catccattac atcacgacga
    16621 ccaccgaatg cgcctaccgg cattccaccg ccgatgattt tgccgaggca ggttaaatct
    16681 ggcactacgc cgtaataatc ctgtgcgcca gctagcgcta cgcggaaacc ggtcatcact
    16741 tcatcgatga tcagcaacgc gccaaattcg tcgcacagcg cgcgcagacc tggcaggaac
    16801 tctggcagcg gcggaacaca gttcatattg cctgccaccg gctcgacgat aatacaggca
    16861 atctcttgcg ggtattgctc aaatgcggcg cgtacagaag ccagatcatt ataagtacag
    16921 gttaaggtat gtttggcgaa atctgccgga acgcccggcg agtttggctg gcctaacgtg
    16981 agtgcgccag aaccggcttt caccagcagg cagtcagcgt gaccatggta acacccttca
    17041 aatttaataa ttttgtcgcg accggtaaaa ccacgggcca ggcggatggc gctcatggtc
    17101 gcttcagtgc cggagttcac catgcgcacc atatccatgg tcgggaccag ttcggtcacc
    17161 agttgcgcca ttttcacttc catttcggtt ggtgcaccaa agcttaaacc acgctcggcg
    17221 gcttcaatca cggcattgcg gattgccgga tggttatggc ccagcaccat cggcccccag
    17281 gaaccgacat aatcgatata ggctttgcca tcaacatcgt acagataagc gccgtccgct
    17341 ttttcgataa acagtggagt gccgcccacg ccagtaaagg cgcgaacagg ggagttcaca
    17401 ccgccaggga tcagctcgcg cgctgcgctg taaagatttt cagacttact catggagggt
    17461 tcctgattcg tagaaaaagt gaatggctgc tattctatgt tattcataac aagttaaata
    17521 ctcgtcaaac atcaggctgc ttgtactggt gcaatcctga tttcttagag tataaaagtt
    17581 ttgtgcattt gaaacattac gctttgcaaa ggattttcat ggaacgtgcg agtaaaatgc
    17641 cgtcatctta tttgtatgac caataagtga tcattggatg aaaactgata ctccctcttt
    17701 agaaacaccg caggccgcgc gcctgcgacg cagacaactg attcgccaac ttcttgagcg
    17761 cgataaaacc ccgttagcca ttttgtttat ggcggcagtc gtcggcacgc ttgtcgggct
    17821 ggcagcggtt gcttttgaca aaggtgtcgc ctggttgcag aaccaacgta tgggggcgct
    17881 ggtacatact gctgataatt atccgcttct gttaaccgtc gcttttctct gttcggcggt
    17941 gctggcgatg ttcggctact ttttggtgcg caaatacgcg ccggaagcag gtggttcggg
    18001 gatcccggaa attgaagggg cgctggaaga tcaacgtccc gttcgctggt ggcgtgtgtt
    18061 gccggtgaaa ttctttggcg ggctggggac actcggcgga ggcatggtgc tagggcgcga
    18121 aggaccaacc gtgcagatcg gcggcaatat cggtcgcatg gtgcttgata ttttccgcct
    18181 gaaaggtgat gaagctcgcc atacgctgct ggcaaccggt gctgctgcgg ggctggctgc
    18241 ggcctttaac gcgccgctgg cgggtatttt gtttattatc gaagagatgc gtccgcagtt
    18301 tcgctatacg ttaatttcga ttaaagcggt atttattggc gtcattatgt cgaccattat
    18361 gtaccggatt tttaatcatg aagttgcgtt gattgacgtc ggtaaacttt ctgatgcgcc
    18421 gcttaatacg ctgtggcttt atctgatcct cggtattatt tttggcattt tcggccctat
    18481 ttttaataaa tgggtgctgg ggatgcagga tttgctgcac cgtgtgcacg gcggcaatat
    18541 taccaaatgg gtactgatgg gtggtgcgat tggcggtctg tgtggattgc tggggtttgt
    18601 ggcaccagca acgtcgggcg gcggttttaa cctgattcct atcgctaccg cggggaattt
    18661 cagcatggga atgctggtgt ttatcttcgt cgctcgggtc attaccacct tactctgctt
    18721 ctcttccggc gcgccgggcg gtatttttgc cccgatgctg gcgctgggta ctgtgctggg
    18781 aaccgctttc ggaatggttg ccgttgagct gtttccgcaa tatcacctgg aggcggggac
    18841 gtttgctatt gccggaatgg gggcattact ggcggcatct attcgcgcgc cgttaacggg
    18901 gatcattctg gttctggaga tgaccgataa ctaccagctc attttgccaa tgattattac
    18961 cggtcttggc gcaacactat tagcgcaatt taccggcggg aaacctctat actcggcgat
    19021 tcttgcgcgc acgctggcaa aacaggaagc tgagcaactg gcgcgaagca aggccgcatc
    19081 agccagcgag aatacttgaa cgaaatacca gggtattaga taatggcgat tattattggg
    19141 ttagaatttg cccaattgcc gatgtcgttt ggagcaaaat atgagtgatg acgtagcact
    19201 gccgctggag tttaccgacg cagcagccaa caaagttaaa agcctgatcg ctgacgaaga
    19261 taacccgaat ctgaaattac gcgtgtatat caccggtggc ggttgcagcg gcttccagta
    19321 tggtttcacc tttgatgatc aggtgaacga aggcgatatg accatcgaaa aacagggcgt
    19381 tggcctggtg gttgatccga tgagcctgca atatctggtc ggcggttccg ttgactacac
    19441 cgaaggactg gaaggttctc gtttcatcgt gaccaacccg aacgcgaaaa gcacctgcgg
    19501 ttgcggttct tcctttagca tctaatctgt tgttcatgtg ccggataaac tatccggcac
    19561 atcttctctc ttcaacgccc attctcatcc agcgcaaacg tcggtagctt aagatgccaa
    19621 cgaatagccg ccagccgaat caatagcgtc acgaccatgc ccatcatact ggctgtttcc
    19681 agtggtacgg aaaatgtgta ataagccgta gcgtggacaa taccgccgat aatacaggca
    19741 gttgcgtaga tttctgtacg taaaatcatg gggatctcgc gggccagtac atcacgaatg
    19801 atcccgccgc caacgccagt aatgacgccc atgcaaaccg cgattaacgg accggcttcc
    19861 gcattaaagg ctttattcac gccaatgccg acaaacaccg ccagaccaac ggcgtccagc
    19921 accggcaaca tccattttgg taagcgtctt ggctggcgca ccagcacgat ggtcagcatg
    19981 ctggtgacca ttgcaacgac cagatcggtg ggatctttca cccaaaatac cgggccgtga
    20041 tccagcgcca tgtcgcgaat tgtcccgccg cctactgcgg ttaccacgcc cagtaccaga
    20101 acaccaaaag ggtccatacg caattttccg gctaacaaaa cgccggagat ggcaaatacc
    20161 gctgtgccga ctatatccag ccaatagacg agcattgttc aatccccact gagcacctga
    20221 aaaggtcagg cgctaatcta cctgtgaaag cgcattacag agctgttgtg cagcgaggat
    20281 aatacgtggg cttgcacgtt caaaccagtc actcgtgaga ggaataacgg gaattttgag
    20341 ctgttcaccc cagtattgtt tgattttagg aatttggtcc ggtccgcctg taatgacaat
    20401 cgcctgtggc gagcgtgcta acacctgttc gcggctaact tgcggccagg gaacccggct
    20461 gtctttaaag atgttttctc cgccacaaac ttcgagtacc tggttctgaa tcgactcttt
    20521 tccactggta aatggcggat taatgccgaa ttgcagaaaa acacgttttt taggtttatc
    20581 agcatattgc gctttcaatt gcgcgtactg atcctgcagg gattgcgcgg cttgttcggc
    20641 cttgtctggt tgcggactcc agggggccag ttgacgtaac gcattggcaa tttgttcaat
    20701 gcttgtcgca tcgacccaca tcacttttat tcccagcgaa gccagctggt caacctgccg
    20761 ctcggcatta cctccacgcc aggcaatcac cagatcgggt ttcagcgcga caatgcgttc
    20821 cagattcatc ccctgccagg tggaaacctg ctcaatcttt tgcgcttgtg gaggatagtc
    20881 ggaatagctg ctgaccccaa ccggcgtgat cccggcggca aaggcaagtt cagtgttggc
    20941 gggagaaagc gtgatgacgc gcggcgcggc gttgagccac agtggcgcaa gaaaagacag
    21001 ggcgaccagc gccctgaaca gtgacttagc catgtgcaag tttctgcacc agtgactcaa
    21061 ccatcaggct ggactgttta gcggcaacag ccaggaactc atcgaagcta agatgagact
    21121 gttgatcggc cacgtcggag atggcgcgta cgacaacaaa cgggacgttg aaattgtggc
    21181 agacatgggc gattgccgtc gcttccatct ctacagcaat ggcctgtggg aagttgtggc
    21241 ggattttcgc cagaccaaca gaaccgttga tgaaagcgtc gccgctaaca atcaggccac
    21301 gtacagcgtt aagattcagt tcggcaatgc aggcctcagc ggcagcgatc agtttatcgt
    21361 cagctttaaa gcctgccgga cagcctggta actgaccgta ttcataacca aatgccgtga
    21421 catccgcgtc gtgataacgt gcttcgtccg agacaacgat atcgcccact ttcagcgttg
    21481 gtgccaggcc accggcagaa ccggtgttaa taatcacatc tggcttgcag tgttccaaca
    21541 gcaaagtggc acccagcgcc gcagcgactt taccgatgcc cgatttcaga agcgcaacct
    21601 cggttccatt cagttggccg gtatagattt cgcaaccgcc cagactgata gtttgacggt
    21661 tttcgatttt gtcacgcagc agcgtaactt cttcttccat tgcaccaatg atgccgattt
    21721 tcatagattt actcgcgata agcccgattt gaaggcatag tttaccatgc gcttacgggg
    21781 aagcgtattt ctcacgcggg agaggacatg gcacagattg atttccgaaa aaaaataaac
    21841 tggcatcgtc gttaccgttc accgcagggc gttaaaaccg aacatgagat cctgcggatc
    21901 ttcgagagcg atcgcgggcg tatcatcaac tctccggcaa ttcgtcgtct gcaacaaaag
    21961 acgcaggttt ttccactgga gcgcaatgcc gccgtgcgca cgcgtcttac ccactcgatg
    22021 gaagtccagc aggtggggcg ctacatcgcc aaagaaattt taagccgtct gaaagagctt
    22081 aaattgctgg aagcatacgg cctggatgaa ctgaccggac cttttgaaag cattgttgag
    22141 atgtcatgcc tgatgcacga tatcggcaat ccgccgtttg gtcattttgg cgaagcagcg
    22201 ataaatgact ggtttcgcca acgtttgcac ccggaagatg ccgaaagcca gcctctgact
    22261 gacgatcgct gcagcgtggc ggcactacgt ttacgggacg gggaagaacc gcttaacgag
    22321 ctgcggcgca agattcgtca ggacttatgt cattttgagg ggaatgcaca aggcattcgt
    22381 ctggtgcata cattgatgcg gatgaatctc acctgggcac aggttggcgg tattttaaaa
    22441 tatacccgtc cggcgtggtg gcgtggcgaa acgcctgaga cacatcacta tttaatgaaa
    22501 aagccgggtt attatctttc tgaagaagcc tatattgccc ggttgcgtaa agaacttaat
    22561 ttggcgcttt acagtcgttt tccattaacg tggattatgg aagctgccga cgatatctcc
    22621 tattgtgtgg cagaccttga agatgcagta gagaaaagaa tatttaccgt tgagcagctt
    22681 tatcatcatt tgcacgaagc gtggggccag catgagaaag gttcgctctt ttcgctggtg
    22741 gttgaaaatg cctgggaaaa atcacgctca aatagtttaa gccgcagtac ggaagatcag
    22801 ttttttatgt atttacgggt aaacacccta aataaactgg taccctacgc ggcacaacga
    22861 tttattgata atctgcctgc gattttcgcc ggaacgttta atcatgcatt attggaagat
    22921 gccagcgaat gcagcgatct tcttaagcta tataaaaatg tcgctgtaaa acatgtgttt
    22981 agccatccag atgtcgagca gcttgaattg cagggctatc gggtcattag cggattatta
    23041 gagatttatc gtcctttatt aagcctgtcg ttatcagact ttactgaact ggtagaaaaa
    23101 gaacgggtga aacgtttccc tattgaatcg cgcttattcc acaaactctc gacgcgccat
    23161 cggctggcct atgtcgaggc tgtcagtaaa ttaccgtcag attctcctga gtttccgcta
    23221 tgggaatatt attaccgttg ccgcctgttg caggattata tcagcggtat gaccgacctc
    23281 tatgcgtggg atgaataccg acgtctgatg gccgtagaac aataaccagg cttttgtaaa
    23341 gacgaacaat aaatttttac cttttgcaga aactttagtt cggaacttcg cgctataaaa
    23401 cgaatctgaa gaacacagca attttgcgtt atctgttaat cgagactgaa atacatgaaa
    23461 aaaaccacat tagcactgag tgcactggct ctgagtttag gtttggcgtt atctccgctc
    23521 tctgcaacgg cggctgagac ttcttcagca acgacagccc agcagatgcc aagccttgca
    23581 ccgatgctcg aaaaggtgat gccttcagtg gtcagcatta acgtagaagg tagcacaacc
    23641 gttaatacac cgcgtatgcc gcgtaatttc cagcagttct tcggtgatga ttctccgttc
    23701 tgccaggaag gttctccgtt ccagagctct ccgttctgcc aggggggcca gggcggtaat
    23761 ggcggcggcc agcaacaaaa attcatggcg ctgggttccg gcgtcatcat tgatgccgat
    23821 aaaggctatg tcgtcaccaa caaccatgtt gttgataacg cgacggtgat taaagtccag
    23881 ctgagcgatg gtcgtaagtt cgacgcgaag atggttggca aagatccgcg ctctgatatc
    23941 gcgctgatcc aaatccagaa cccgaaaaac ctgaccgcaa ttaagatggc ggattctgat
    24001 gcactgcgcg tgggtgatta caccgtagcg attggtaacc cgtttggtct gggagagacg
    24061 gtaacttccg ggattgtctc tgcgctgggg cgtagcggcc tgaatgccga aaactacgaa
    24121 aacttcatcc agaccgatgc agcgatcaac cgtggtaact ccggtggtgc gctggttaac
    24181 ctgaacggcg aactgatcgg tatcaacacc gcgatcctcg caccggacgg cggcaacatc
    24241 ggtatcggtt ttgctatccc gagcaacatg gtgaaaaacc tgacctcgca gatggtggaa
    24301 tacggccagg tgaaacgcgg tgagctgggt attatgggga ctgagctgaa ttccgaactg
    24361 gcgaaagcga tgaaagttga cgcccagcgc ggtgctttcg taagccaggt tctgcctaat
    24421 tcctccgctg caaaagcggg cattaaagcg ggtgatgtga tcacctcact gaacggtaag
    24481 ccaatcagca gctttgccgc actgcgtgct caggtgggta ctatgccggt gggcagcaaa
    24541 ctgaccctgg gcttgctgcg cgacggtaag caggtcaacg tgaatctgga actgcagcag
    24601 agcagccaga atcaggttga ttccagctcc atcttcaacg gcattgaagg tgctgagatg
    24661 agcaacaaag gcaaagatca gggcgtggta gtgaacaacg tgaaaacggg cactccggct
    24721 gcgcagatcg gcctgaagaa aggtgatgtg attattggcg cgaaccagca ggcagtgaaa
    24781 aacatcgctg aactgcgtaa agttctcgac agcaaaccgt ctgtgctggc actgaacatt
    24841 cagcgcggcg acagctccat ctacctgtta atgcagtaat ctccctcaac cccttcctga
    24901 aaacgggaag gggttctcct tacaatctgt gaacttcacc acaactccat acatcttcat
    24961 catcctttag gcatttgcac aatgccgtac gttacgtact tccttatgct aagccgtgca
    25021 taacggagga cttatggctg gctggcatct tgataccaaa atggcgcagg atatcgtggc
    25081 acgtaccatg cgcatcatcg ataccaatat caacgtaatg gatgcccgcg ggcgaattat
    25141 cggcagcggc gatcgtgagc gtattggtga attgcacgaa ggtgcattgc tggtactttc
    25201 acagggacga gtcgtcgata tcgatgacgc ggtggcacgc catctgcacg gtgtgcggca
    25261 ggggattaat ctaccgttac ggctggaagg tgaaattgtc ggcgtaattg gcctgacagg
    25321 tgaaccagag aatctgcgta aatatggcga actggtctgc atgacggctg aaatgatgct
    25381 ggaacagtcg cggttgatgc acctgttggc gcaggatagc cgtttgcggg aagaactggt
    25441 gatgaacctg attcaggctg aggagaatac tcccgcactt actgaatggg cgcaacggct
    25501 ggggatcgat ctcaatcaac cgcgagtagt ggctattgtt gaggtcgaca gcggtcagct
    25561 tggcgtggac agcgcaatgg cggagttaca acaactgcaa aatgcgctga ctacgcccga
    25621 gcgtaataat ctggtggcga ttgtctcgct gaccgaaatg gtggtgttga aaccggcgtt
    25681 gaactctttt gggcgctggg atgcagaaga tcatcgtaag cgagttgaac aactgattac
    25741 ccgcatgaaa gagtatggcc agctgcgttt tcgcgtttcg ctgggcaact attttaccgg
    25801 tcctggcagt attgcccgat cctatcgtac ggcgaaaacg acgatggtgg tgggtaaaca
    25861 gcggatgcca gaaagtcgct gctattttta tcaggatctg atgttacctg tgttactcga
    25921 cagtttgcgt ggcgactggc aggccaatga actggcgcga ccgctggcgc ggctgaaagc
    25981 gatggacaat aatggcttgc tgcgacgaac gctggcggcg tggtttcgcc acaatgtgca
    26041 accgctggct acgtcaaagg cgctgtttat tcatcgtaat actctggagt atcggcttaa
    26101 tcgtatatcg gaactgaccg ggcttgattt gggtaatttt gatgacaggt tgctgctgta
    26161 tgtggcgttg cagctggatg aagagcggta ggttatgcgt taaggtcgag gcggcgctgg
    26221 ctcattcccc tcagcccgaa gggtagaggg gaatgtccga gttaaccgtc agttcggcat
    26281 tatttacgcg ttagtttttc cagatcggct tcaatctcgc tgatcttatt ggttacgact
    26341 gactccaggt gacgtaagtc gtcgaggatc ttacgcttaa gatcaacttc gctgcggtca
    26401 cgctggcaga tttgatcaag ctcatcaatg atataccgta gattcgggct gatttcctgg
    26461 acttctttat aaccctgacc cacaccatca gcgacgaccg ttttacgctg acgcggatat
    26521 ttaaacttaa cgctcttggc gaaaaactcg cctttgtctt tctggaaata gattttcaga
    26581 atatcgttgt tggcttcctg ccggaggctg taacgatcaa tttcttcagg attggtaata
    26641 cccagacttt tcagattgtc gtacatagcg ttacctcaaa atgagtcagt aaattgtgct
    26701 tattttagca tttggcctgg cccgcccggc tcgactgtga tcgcagaaag cctttttctt
    26761 ccagacttct atcatggcgt aaaaatcaaa aaattacctg ctttattgtg gcgatattcc
    26821 atgggatgga ttaacacatt tatcttcgtt ttgcgaatta catgacagtc cgaaacaaga
    26881 tgaaaaagta tttaaaaaag gggggaagaa gaatgagtag gacgaattat tctgagccag
    26941 aaagcatacg ggcagcatca tgctgcccgt atatgtactc ttaatcgatg gtacgcagca
    27001 gttcgttaat gccgactttg ccgcgagttt tcgcgtcaac tttcttaacg ataaccgcac
    27061 agtagaggct gtatttgcca tctttcgacg gcagattacc tgaaacaacc acagaccccg
    27121 ccggaacgcg accgtagtgg atttcgccgg tttcacggtc gtaaatacgg gtgctctgac
    27181 caatgtatac gcccatagag atgacggaac cttcttcgac aatcacccct tcaaccactt
    27241 cggagcgtgc gccaatgaag cagttatctt caataatggt cgggttagcc tgcagcggct
    27301 ccagcacgcc gccgatgccc acgccaccgg aaaggtggac gtttttacca atctgcgcac
    27361 aagaaccgac ggtcgcccag gtatcaacca tcgtgccttc atcaacatat gcgccgatgt
    27421 tgacgtaaga cggcatcagc acggtgttac gggcaataaa tgcaccctga cgtaccgccg
    27481 ctggtggcac aacgcggaag ccttctttct gaaaacgcgc ttcgtcgtag tcggcgaatt
    27541 tcatcggcac tttatcgaag tagcggcttt ctgccccttc gatcacctga ttatcattaa
    27601 tacggaaaga gagcagcacc gcttttttca accactgatg cgtcacccac tgaccgtcaa
    27661 ttttttccgc tacacgcagt gcgccggaat ccagcagggc gatcacctga tttaccgctt
    27721 cgcgggttac ggtgtctgca ttggctggcg tgatctcggc acggcgttca aaagcggttt
    27781 caataatgtt ctgtaactgc tgcattgtta aactcttttc atatcagtaa acacatcacc
    27841 ctttatcgtt tggattgagg gcctctgtca accgctgatg cacttcctgc tgcaactcgt
    27901 tattaagtgc acgccggtca gcggtggcaa ttatgaataa atcttctact cgctcgccaa
    27961 tggttgtaat tcgggcacca tgaagcgaaa ttcccagatc ggcaaaaatt ttcccgactc
    28021 tcgccagcag tcctggctgg tcgagcgcga taagctcaag gaacgatttg cggtcggtat
    28081 gggtcggcaa aaacgttact tcggtttcaa cagtaaaatg gcgtaatttg gcgggttggc
    28141 gacggggctg cggtggctgc cagctacttt gcgtcagtac ttgctccaga ccaaaccgaa
    28201 taacctcatg acgatctgcg gacagcgggc tgccatcggg ttccagcacg ataaaggtat
    28261 ccatcgccat accgtcgcga gtggtgaaaa tttgtgcgtc gtgaacactt aaattgcggc
    28321 ggtctaattc ggcacagacg gcggcaaaca gataggggcg gtccgggctc cagataaaaa
    28381 tctcggtgcc tccacgcgta gcctgcgggc taagcaatac cagcggtttg cttaaatcat
    28441 gctgtaataa atggcgggca tgccaggcca gttgatttgg gctatggcgg acaaaatagt
    28501 tagcacgaca gcgtgaccaa atttggtgca gcgcctcttc gtcgatgtta tccatgcgca
    28561 gtagtgccag tgcctggagt tggtgatggc gaacccgttc gcgcatatcc ggcgtgtttt
    28621 gcattccgcg tcgtagctgc ttttcggtgg caaaatagag ttcgcgcaac agactttgct
    28681 tccagctatt ccacagcgtt tcgttggtgg cgcaaatgtc agccactgtc aggcatacca
    28741 gatagcgcag acgattttcc gtttgaactt cttcggcaaa ctgcttgatg acttcagggt
    28801 cctgaatatc gcggcgttgt gctgtcaccg acatcagcaa gtgctggcga accagccagg
    28861 caaccagctg tgtttcgcgt gagttcagcc cgtggagttc tgcaaaatgc actacatcct
    28921 gagcaccgag aatggagtgg tcgccgccgc gtcctttggc gatatcgtga aacagcgcgg
    28981 cgatgaaaat cagctcagtt gacggcaggc gcggccagac gtccacacac aacggatggc
    29041 gctggcgcgt ttcttcactg gcaaaactct ccagtttcag catcacgcgg atagtatgtt
    29101 catccaccgt gtaggcgtgg aacagatcaa actgcatctg cccgacgata tgcgaccatt
    29161 gcggcatata cgcgccgagc acgctatggc gatgcattgg caatagcccg cgccgcaccg
    29221 ctccggggtg acgcagaatg ctcaaaaaca gttttcgtgc ttccggaata ttacacagcg
    29281 gttgttgcag atggcgacgg gcatggcgta actggcgcag cgtggtggag taaatgccgg
    29341 tgatcgcact gttgcgcacc atggtgtaga acatacgcaa gatggcttcc ggctggcgca
    29401 taaatagtgt ttcatcacgc aggtcgatta gcgtaccgcg tagctgaaac tcatcgtcga
    29461 ttggacgtgg tttttcgtcg gcgggaaggg cgaggatggc ttcatcgaac agttgcagca
    29521 gcatctggtt gagttcactg acgcggcgtg taacgcggaa gtaatccttc atcatccgct
    29581 cgaccggttc gttgccttca ccactgtaat tcagacgctg agcgacgcta agttggcgat
    29641 cgaataacag acgattatcg taacggctga cgaccagatg cagggcaaag cgaatacgcc
    29701 acagtatatg cagacattcg tttaattccg cccgctccgc tgaggttaag aagccaaacc
    29761 cgaccatttc atccagcgat gttgcgccaa aatgacggcg ggccacccat tgcagagtgt
    29821 gaatatcgcg caagccgcca gggctacttt tgatgtctgg ttcaaggttg tagctggtgc
    29881 catggtaacg ctgatggcgc tggttctgtt cttcaacttt cgccgcgtag aacttgtcgg
    29941 aaggccagaa tccttcgcta aaaatatgtt tttgcagttc gaggaagagc gcaacatcgc
    30001 caattaataa gcgggattcg attaaattgg tggcgacggt taaatccgat aacccttcca
    30061 gcatgcactc ttcaagcgtg cgcacactat gaccgacttc cagctttaca tcccagagca
    30121 gcgttaacag ctcgcccact ttttgcgcct gatcgtccgg gagcttttta cggcttaaaa
    30181 tcagtaaatc gatgtcagaa agtggatgca gctcgccacg accgtagcca ccgacggcga
    30241 ccaatgccag gtcggcaatc tggctgaatc ccgcttcaat ccataatcgt tgcaggagct
    30301 ggtcgataaa ctcggtgcgc gcctcaatca actgttctgc agagatccca ttgtcaaagg
    30361 catcacccag ccaacgctgg aaagtatcga tatgggcttt tatcccaccg acggttaatt
    30421 catcacgggg ccagacgcat ggattttgcg gttgaccggg cagggtgggg agagcggtgt
    30481 ttgcgtactg ttctggaagg gtattcattg tgcgccaccc ataagattaa attatcgcat
    30541 taaaaaagcc ggcattcgcc ggcttcatct tattcgtcgt gcgagattat cgccgggatg
    30601 gtgtcatcct tgcgtagcgt cagaatttcg cagccgttat cagtcaccac aatagtatgc
    30661 tcatattgtg cagacaagct gcgatctttg gtttttaccg tccagccatc tttcatggtg
    30721 cggatctctt ttttacccgc gttgaccatt ggctcgatgg tgaacgtcat cccaggtttc
    30781 agtacgacgt tggtttcacg ggagtcatag tgcagcacct gcggttcttc atggaagccg
    30841 cgaccaatac cgtgtccgca atattcacga acgacggaga agccttctgc ttcgacaaat
    30901 ttctgaatcg ccgcaccgat ttcgcgcaga ttaatgcctg gtttgaccat gcgcagcgcc
    30961 aggtacaggc tttcttgcgt gatgcggcac agacgttcgc ccatgatggt cggcttaccg
    31021 acgataaaca ttttcgaggt atcgccgtgg aaaccatctt tgattacggt gacatcaatg
    31081 ttaacgatat cgccatcttt cagcagcttc gcatcgtccg ggataccgtg gcacaccact
    31141 tcattaatag agatgcaaac ggatttcgga tagccgtgat agccgaggca ggcagaaacc
    31201 gcgtgttgtt cattaacaat gtaatcatta cagatgcgat ccagctcgcc ggtgctgacg
    31261 cccggtttaa catacggttc gatcatctcc agcacttcgg cagccagtcg gccagcgacg
    31321 cgcatttttt cgatatcttc tggggtcttg attgagatag ccattaattc tgtccatcag
    31381 cgtcggtgat accgacaata tatatgtaag tgccgtcaat ggtatcacac ccgggcaaat
    31441 tgagaatcat tctgaatttc gccaaacgtg ccactgaacg ttttctataa tagaaaattc
    31501 aacgtctgat gctgtacaca gcgccaacaa ttattggtgt ccatgacgta tttgtggtat
    31561 aaagcgcgcc ggacttccga tccatttcgt atacacagac tggacggaag cgacaatctc
    31621 actttgtgta acaacacaca cgtatcggca catattccgg ggtgcccttt ggggtcggta
    31681 atatgggata cgtggaggca taaccccaac ttttatatag aggttttaat catggcaact
    31741 gtttccatgc gcgacatgct caaggctggt gttcacttcg gtcaccagac ccgttactgg
    31801 aacccgaaaa tgaagccgtt catcttcggt gcgcgtaaca aagttcacat catcaacctt
    31861 gagaaaactg taccgatgtt caacgaagct ctggctgaac tgaacaagat tgcttctcgc
    31921 aaaggtaaaa tccttttcgt tggtactaaa cgcgctgcaa gcgaagcggt gaaagacgct
    31981 gctctgagct gcgaccagtt cttcgtgaac catcgctggc tgggcggtat gctgactaac
    32041 tggaaaaccg ttcgtcagtc catcaaacgt ctgaaagacc tggaaactca gtctcaggac
    32101 ggtactttcg aaaagctgac caagaaagaa gcgctgatgc gcactcgtga gctggagaaa
    32161 ctggaaaaca gcctgggcgg tatcaaagac atgggcggtc tgccggacgc tctgtttgta
    32221 atcgatgctg accacgaaca cattgctatc aaagaagcaa acaacctggg tattccggta
    32281 tttgctatcg ttgataccaa ctctgatccg gacggtgttg acttcgttat cccgggtaac
    32341 gacgacgcaa tccgtgctgt gaccctgtac ctgggcgctg ttgctgcaac cgtacgtgaa
    32401 ggccgttctc aggatctggc ttcccaggcg gaagaaagct tcgtagaagc tgagtaataa
    32461 ggcttgataa ctccctcaaa atagttcgag ttgcagaaag gcggcaagct cgagaattcc
    32521 cgggagctta catcagtaag tgaccgggat gagcgagcga agacaacgca tctgcggcgc
    32581 gaaatatgaa gggggagagc ccttatagac caggtagtac acgtttggtt agggggcctg
    32641 catatggccc cctttttcac ttttatatct gtgcggttta atgccgggca gatcacatct
    32701 ccgaggattt tagaatggct gaaattaccg catccctggt aaaagagctg cgtgagcgta
    32761 ctggcgcagg catgatggat tgcaaaaaag cactgactga agctaacggc gacatcgagc
    32821 tggcaatcga aaacatgcgt aagtccggtg ctattaaagc agcgaaaaaa gcaggcaacg
    32881 ttgctgctga cggcgtgatc aaaaccaaaa tcgacggcaa ctacggcatc attctggaag
    32941 ttaactgcca gactgacttc gttgcaaaag acgctggttt ccaggcgttc gcagacaaag
    33001 ttctggacgc agctgttgct ggcaaaatca ctgacgttga agttctgaaa gcacagttcg
    33061 aagaagaacg tgttgcgctg gtagcgaaaa ttggtgagaa catcaacatt cgccgcgttg
    33121 ctgcgctgga aggcgacgtt ctgggttctt atcagcacgg tgcgcgtatc ggcgttctgg
    33181 ttgctgctaa aggcgctgac gaagagctgg ttaaacacat cgctatgcac gttgctgcaa
    33241 gcaagccaga attcatcaaa ccagaagacg tatccgctga agtggtagaa aaagaatacc
    33301 aggtacagct ggatatcgcg atgcagtctg gtaagccgaa agaaatcgca gagaaaatgg
    33361 ttgaaggccg catgaagaaa ttcaccggcg aagtttctct gaccggtcag ccgttcgtta
    33421 tggaaccaag caaaactgtt ggtcagctgc tgaaagagca taacgctgaa gtgactggct
    33481 tcatccgctt cgaagtgggt gaaggcatcg agaaagttga gactgacttt gcagcagaag
    33541 ttgctgcgat gtccaagcag tcttaattat cgaaaaggag ccgcctgagg gcggcttctt
    33601 tttgtgccca tcttgtaaat tcagctaacc cttgtggggc tgcgctgaaa agcgacgtac
    33661 aatgtcgcta gtattaattc atttcaatcg ttgacagtct caggaaagaa acatggctac
    33721 caatgcaaaa cccgtctata aacgcattct gcttaagttg agtggcgaag ctctgcaggg
    33781 cactgaaggc ttcggtattg atgcaagcat actggatcgt atggctcagg aaatcaaaga
    33841 actggttgaa ctgggtattc aggttggtgt ggtgattggt gggggtaacc tgttccgtgg
    33901 cgctggtctg gcgaaagcgg gtatgaaccg cgttgtgggc gaccacatgg ggatgctggc
    33961 gaccgtaatg aacggcctgg caatgcgtga tgcactgcac cgcgcctatg tgaacgctcg
    34021 tctgatgtcc gctattccat tgaatggcgt gtgcgacagc tacagctggg cagaagctat
    34081 cagcctgttg cgcaacaacc gtgtggtgat cctctccgcc ggtacaggta acccgttctt
    34141 taccaccgac tcagcagctt gcctgcgtgg tatcgaaatt gaagccgatg tggtgctgaa
    34201 agcaaccaaa gttgacggcg tgtttactgc tgatccggcg aaagatccaa ctgcaaccat
    34261 gtacgagcaa ctgacttaca gcgaagtgct ggaaaaagag ctgaaagtca tggacctggc
    34321 ggccttcacg ctggctcgtg accataaatt accgattcgt gttttcaaca tgaacaaacc
    34381 gggtgcgctg cgccgtgtgg taatgggtga aaaagaaggg actttaatca cggaataatt
    34441 cccgtgatgg ataaataagg gtaagattcc gcataagtat cgcgggggcg taagtctggt
    34501 tataaggcgt tattgttgca ggcagtttgg tcacggccag cgcgcagcaa ccggtgcgta
    34561 cacaagtacg tgaggatggc gagcactacc cggggccaaa atggcaaata aaatagccta
    34621 ataatccaga cgattacccg taatatgttt aatcagggct atacttagca cacttccact
    34681 gtgtgtgact gtctggtctg actgagacaa gttttcaagg attcgtaacg tgattagcga
    34741 tatcagaaaa gatgctgaag tacgcatgga caaatgcgta gaagcgttca aaacccaaat
    34801 cagcaaaata cgcacgggtc gtgcttctcc cagcctgctg gatggcattg tcgtggaata
    34861 ttacggcacg ccgacgccgc tgcgtcagct ggcaagcgta acggtagaag attcccgtac
    34921 tctgaaaatc aacgtgtttg atcgttcaat gtctccggcc gttgaaaaag cgattatggc
    34981 gtccgatctt ggcctgaacc cgaactctgc gggtagcgac atccgtgttc cgctgccgcc
    35041 gctgacggaa gaacgtcgta aagatctgac caaaatcgtt cgtggtgaag cagaacaagc
    35101 gcgtgttgca gtacgtaacg tgcgtcgtga cgcgaacgac aaagtgaaag cactgttgaa
    35161 agataaagag atcagcgaag acgacgatcg ccgttctcag gacgatgtac agaaactgac
    35221 tgatgctgca atcaagaaaa ttgaagcggc gctggcagac aaagaagcag aactgatgca
    35281 gttctgattt cttgaacgac aaaaacgccg ctcagtagat ccttgcggat cggctggcgg
    35341 cgttttgctt tttattctgt ctcaactctg gatgtttcat gaagcaactc accattctgg
    35401 gctcgaccgg ctcgattggt tgcagcacgc tggacgtggt gcgccataat cccgaacact
    35461 tccgcgtagt tgcgctggtg gcaggcaaaa atgtcactcg catggtagaa cagtgcctgg
    35521 aattctctcc ccgctatgcc gtaatggacg atgaagcgag tgcgaaactt cttaaaacga
    35581 tgctacagca acagggtagc cgcaccgaag tcttaagtgg gcaacaagcc gcttgcgata
    35641 tggcagcgct tgaggatgtt gatcaggtga tggcagccat tgttggcgct gctgggctgt
    35701 tacctacgct tgctgcgatc cgcgcgggta aaaccatttt gctggccaat aaagaatcac
    35761 tggttacctg cggacgtctg tttatggacg ccgtaaagca gagcaaagcg caattgttac
    35821 cggtcgatag cgaacataac gccatttttc agagtttacc gcaacctatc cagcataatc
    35881 tgggatacgc tgaccttgag caaaatggcg tggtgtccat tttacttacc gggtctggtg
    35941 gccctttccg tgagacgcca ttgcgcgatt tggcaacaat gacgccggat caagcctgcc
    36001 gtcatccgaa ctggtcgatg gggcgtaaaa tttctgtcga ttcggctacc atgatgaaca
    36061 aaggtctgga atacattgaa gcgcgttggc tgtttaacgc cagcgccagc cagatggaag
    36121 tgctgattca cccgcagtca gtgattcact caatggtgcg ctatcaggac ggcagtgttc
    36181 tggcgcagct gggggaaccg gatatgcgta cgccaattgc ccacaccatg gcatggccga
    36241 atcgcgtgaa ctctggcgtg aagccgctcg atttttgcaa actaagtgcg ttgacatttg
    36301 ccgcaccgga ttatgatcgt tatccatgcc tgaaactggc gatggaggcg ttcgaacaag
    36361 gtcaggcagc gacgacagca ttgaatgccg caaacgaaat caccgttgct gcttttcttg
    36421 cgcaacaaat ccgctttacg gatatcgctg cgttgaattt atccgtactg gaaaaaatgg
    36481 atatgcgcga accacaatgt gtggacgatg tgttatctgt tgatgcgaac gcgcgtgaag
    36541 tcgccagaaa agaggtgatg cgtctcgcaa gctgaggata atccggctac agagagtcgc
    36601 gctatttgtt agcgtagggc ttcagtgata tagtctgcgc catctgatcg taagtagttg
    36661 gctttataag gtcaggtatg ccgtggtttt acacggcttt tttttgtata ggcttcagta
    36721 ttcctgagta ccgtaaaccc tgtcagggaa taaaaaacgc gtgatgttgt ctgctactca
    36781 accacttagc gaaaaattgc cagcgcatgg ctgccgtcat gttgcgatca ttatggacgg
    36841 caatggtcgc tgggcaaaaa agcaagggaa gattcgtgcc tttgggcata aagccggggc
    36901 aaaatccgtc cgccgggctg tctcttttgc ggccaacaac ggtattgagg cgttaacgct
    36961 gtatgccttt agtagtgaaa actggaaccg accagcgcag gaagtcagtg cgttaatgga
    37021 actgtttgtg tgggcgctcg atagcgaagt aaaaagtctg caccgacata acgtgcgtct
    37081 gcgtattatt ggcgatacca gtcgctttaa ctcgcgtttg caagaacgta ttcgtaaatc
    37141 tgaagcgcta acagccggga ataccggtct gacgctgaat attgcggcga actacggtgg
    37201 acgttgggat atagtccagg gagtcaggca actggctgaa aaggtgcagc aaggaaacct
    37261 gcaaccagat cagatagatg aagagatgct aaaccagcat gtctgtatgc atgaactggc
    37321 ccctgtagat ttagtaatta ggactggggg ggagcatcgc attagtaact ttttgctttg
    37381 gcaaattgcc tatgccgaac tttactttac agatgttctc tggcccgatt tcgatgaaca
    37441 agactttgaa ggggcgttaa atgcctttgc taatcgagag cgtcgtttcg gcggcaccga
    37501 gcccggtgat gaaacagcct gatgggggtc gcttttgctg aagtatcgcc tgatatctgc
    37561 ttttgtgtta atacccgtcg tcatcgcggc gttgtttctg ttgccgccgg tggggttcgc
    37621 catcgtaacg ctggtggtct gcatgctggc agcgtgggaa tggggacagc ttagcggttt
    37681 taccactcgt tcgcagcgag tatggttggc ggtgttatgc gggttattgt tggcgctgat
    37741 gctttttctg ttgccggaat atcaccgaaa tattcatcaa ccgctggttg aaatctcact
    37801 ttgggcttcg ctgggttggt ggattgtcgc gctattgctg gtgctgtttt acccaggttc
    37861 cgcagcaatc tggcgtaact ctaaaacatt gcgccttatt tttggcgtgc taaccattgt
    37921 tcccttcttc tggggcatgc tggcgttacg ggcctggcac tatgacgaga atcattacag
    37981 tggcgcaata tggctgctct atgtcatgat cctggtatgg ggcgctgact ccggcgcata
    38041 tatgtttggc aaattgtttg gtaaacataa gctggcaccg aaggtttctc cgggtaaaac
    38101 ctggcaaggc tttataggtg gactagcgac cgcagcggta atctcatggg gttatggcat
    38161 gtgggcgaat ctcgacgtcg ctcccgtcac cttactcatt tgctctattg tcgcagcgtt
    38221 agcctcagtg ctcggcgatc tgaccgagag tatgtttaag cgtgaagcag gaattaagga
    38281 cagcggtcat ttaattccag gacacggtgg tattttagat cgtattgata gcctgacggc
    38341 tgcggtaccg gtctttgctt gcttgttgtt actggtattc aggacgcttt aacggaaggt
    38401 aatatgctga gttttctctg ggatttggct tcgttcatcg ttgcactggg tgtacttatc
    38461 accgtgcatg aatttggtca tttctgggtt gcccggcgtt gtggtgttcg cgttgagcgt
    38521 ttctcaatag ggtttggtaa ggcgctctgg cggcgaactg ataagctcgg caccgaatat
    38581 gttatcgccc tgatcccgct gggcggttat gtcaaaatgc tggatgagcg cgcagaaccg
    38641 gtcgttccgg aactccgcca ccatgccttc aataataaat ctgttggcca acgagcggcg
    38701 attattgccg caggtccggt tgcaaacttc atttttgcta tctttgccta ctggctggtt
    38761 tttattattg gtgtgcctgg cgtacgtccg gtggttggtg aaatagcagc caattcgata
    38821 gctgcggaag cacaaattgc accaggtacg gaactaaaag ccgtagatgg tatcgaaacg
    38881 cctgattggg atgccgtgcg tttgcagttg gtcgataaaa ttggcgatga aagcaccacc
    38941 attacagtag cgccatttgg cagcgatcaa cggcgggatg taaagctcga tttacgtcac
    39001 tgggcgtttg agcctgataa agaagatccg gtatcttcgc tggggattcg tcctcgtggg
    39061 ccgcaaattg aacctgtact ggaaaatgtg cagccaaact cggcggcaag caaggcaggt
    39121 ttgcaagcag gcgacaggat cgttaaagtc gatggtcagc ccttaacgca gtgggtgacc
    39181 tttgtgatgc ttgtccggga taacccgggt aaatccttag cgttagaaat cgaaaggcag
    39241 ggaagtccct tgtctttgac attaatcccg gagagtaaac cgggtaatgg taaagcgatt
    39301 ggttttgtcg gtattgagcc gaaagtcatt cctttgccag atgagtataa agttgtacgc
    39361 cagtatgggc cgttcaacgc catcgtcgaa gccacggaca aaacgtggca gctgatgaag
    39421 ctgacggtca gtatgctggg aaaattgatc accggtgatg tgaaactgaa caacctcagt
    39481 gggccgatct ctatcgccaa gggggctggg atgacagcgg aactcggggt agtttattac
    39541 ctgccgtttc ttgcgcttat tagcgtgaac ttagggataa ttaacctgtt tccgttgccc
    39601 gtacttgacg gggggcatct gctgttcctt gcgatcgaaa agatcaaggg cggaccggta
    39661 tccgagcggg ttcaagactt ttgttatcgc attggctcga ttctgctggt gctgttaatg
    39721 gggcttgcac ttttcaatga tttctctcgg ttatgagagt tagttaggaa gaacgcataa
    39781 taacgatggc gatgaaaaag ttgctcatag cgtcgctgct gtttagcagc gccaccgtat
    39841 acggtgctga agggttcgta gtgaaagata ttcatttcga aggccttcag cgtgtcgccg
    39901 ttggtgcggc cctcctcagt atgccggtgc gcacaggcga cacggttaat gatgaagata
    39961 tcagtaatac cattcgcgct ctgtttgcta ccggcaactt tgaggatgtt cgcgtccttc
    40021 gtgatggtga tacccttctg gttcaggtaa aagaacgtcc gaccattgcc agcattactt
    40081 tctccggtaa caaatcggtg aaagatgaca tgctgaagca aaacctcgag gcttctggtg
    40141 tgcgtgtggg cgaatccctc gatcgcacca ccattgccga tatcgagaaa ggtctggaag
    40201 acttctacta cagcgtcggt aaatatagcg ccagcgttaa agctgtcgtg accccgctgc
    40261 cgcgcaaccg tgttgaccta aaactggtgt tccaggaagg tgtgtcagct gaaatccagc
    40321 aaattaacat tgttggtaac catgctttca ccaccgacga actgatctct catttccaac
    40381 tgcgtgacga agtgccgtgg tggaacgtgg taggcgatcg taaataccag aaacagaaac
    40441 tggcgggcga ccttgaaacc ctgcgcagct actatctgga tcgcggttat gcccgtttca
    40501 acatcgactc tacccaggtc agtctgacgc cagataaaaa aggtatttac gtcacggtga
    40561 acattaccga aggcgatcag tacaagcttt ctggcgttga agtgagcggc aaccttgccg
    40621 ggcactccgc tgaaattgag cagctgacta agatcgagcc gggcgagctg tataacggca
    40681 ccaaagtgac caagatggaa gatgacatca aaaagcttct cggtcgctat ggttatgcct
    40741 atccgcgcgt acagtcgatg cctgaaatta acgatgccga caaaaccgtt aaattacgcg
    40801 tgaacgttga tgcgggtaac cgtttctacg tgcgtaagat ccgttttgaa ggtaacgata
    40861 cctcgaaaga tgccgtcctg cgtcgcgaaa tgcgtcagat ggaaggtgca tggctgggaa
    40921 gcgatctggt cgatcagggt aaggagcgtc tgaatcgtct gggcttcttt gaaactgtcg
    40981 ataccgatac ccaacgtgtt ccgggtagcc cggaccaggt agatgtcgtc tacaaggtaa
    41041 aagagcgcaa caccggtagc ttcaactttg gtattggtta cggtactgaa agtggcgtga
    41101 gcttccaggc tggtgtgcag caggataact ggttaggtac aggttatgct gttggtatca
    41161 acgggaccaa aaacgattac cagacctatg ctgaactgtc ggtaaccaac ccgtacttca
    41221 ctgtagatgg tgtaagcctc ggtggtcgtc tcttctataa tgacttccag gcagatgacg
    41281 ccgacctgtc cgactatacc aacaagagtt atggtacaga cgtgacgttg ggcttcccga
    41341 tcaacgaata taactcgctg cgtgcaggtc tgggttatgt acataactcc ctgtccaaca
    41401 tgcagcctca ggttgcgatg tggcgttatc tgtactctat gggtgaacat ccgagcacct
    41461 ctgatcagga taatagcttc aaaacggacg acttcacgtt caactatggt tggacctata
    41521 acaagcttga ccgtggttac ttcccaacag atggttcacg tgtcaacctg accggtaaag
    41581 tgaccattcc tggatcggat aacgaatact acaaagtgac gttagacacg gcgacttatg
    41641 tgccgatcga tgacgatcac aaatgggttg ttctggggcg tacccgctgg ggttatggtg
    41701 atggtttagg cggcaaagag atgccgttct acgagaactt ctatgccggt ggttccagca
    41761 ccgtgcgtgg cttccagtcc aatacaattg gtccgaaagc agtttacttc ccgcatcagg
    41821 ccagtaatta tgatccggac tatgattacg aatgtgcgac tcaggacggc gcgaaagacc
    41881 tgtgtaaatc ggatgatgct gtaggcggta acgccatggc ggttgccagc ctcgagttca
    41941 tcaccccgac gccgtttatt agcgataagt atgctaactc ggttcgtact tccttcttct
    42001 gggatatggg taccgtttgg gatacaaact gggattccag ccaatattct ggatatccgg
    42061 actatagtga tccaagcaat atccgtatgt ctgcgggtat cgcattacaa tggatgtccc
    42121 cattggggcc gttggtgttc tcctacgccc agccgttcaa aaagtacgat ggagacaagg
    42181 cagaacagtt ccagtttaac atcggtaaaa cctggtaagt gttctccaca aaggaatgta
    42241 gtggtagtgt agcgatgact ttaggcgatc aatataagat cgccgggcca cgcaaagaac
    42301 tgcaccctcc ggtgcaaatg ggatggtaag gagtttattg tgaaaaagtg gttattagct
    42361 gcaggtctcg gtttagcact ggcaacttct gctcaggcgg ctgacaaaat tgcaatcgtc
    42421 aacatgggca gcctgttcca gcaggtagcg cagaaaaccg gtgtttctaa cacgctggaa
    42481 aatgagttca aaggccgtgc cagcgaactg cagcgtatgg aaaccgatct gcaggctaaa
    42541 atgaaaaagc tgcagtccat gaaagcgggc agcgatcgca ctaagctgga aaaagacgtg
    42601 atggctcagc gccagacttt tgctcagaaa gcgcaggctt ttgagcagga tcgtgcacgt
    42661 cgttccaacg aagaacgcgg caaactggtt actcgtatcc agactgctgt gaaatccgtt
    42721 gccaacagcc aggatatcga tctggttgtt gatgcaaacg ccgttgctta caacagcagc
    42781 gatgtaaaag acatcactgc cgacgtactg aaacaggtta aataagtaat gccttcaatt
    42841 cgactggctg atttagcgca gcagttggat gcagaactac acggtgatgg cgatatcgtc
    42901 atcaccggcg ttgcgtccat gcaatctgca caaacaggtc acattacgtt catggttaac
    42961 ccaaaatacc gtgagcattt aggcttgtgc caggcgtccg cggttgtcat gacccaggac
    43021 gatcttcctt tcgcgaaaag tgccgcgctg gtagtgaaga atccctacct gacttacgcg
    43081 cgcatggcgc aaattttaga taccacgccg cagcccgcgc agaacattgc acccagtgcg
    43141 gtgatcgacg cgacggcgaa gctgggtaac aacgtatcga ttggcgctaa cgcggtgatt
    43201 gagtccggcg ttgaactggg cgataacgtg attatcggtg ccggttgctt cgtaggtaaa
    43261 aacagcaaaa tcggtgcagg ttcgcgtctc tgggcgaacg taaccattta ccatgagatc
    43321 cagatcggtc agaattgcct gatccagtcc ggaacagtgg taggcgcaga cggctttggt
    43381 tatgccaacg atcgtggtaa ctgggtgaag atcccacaga ttggtcgcgt aattattggc
    43441 gatcgcgtgg agatcggtgc ctgcacaacc atcgatcgcg gcgcgctgga tgacactgtt
    43501 attggcaatg gcgtgatcat tgataaccag tgccagattg cacataacgt cgtgattggc
    43561 gacaatacgg cggttgccgg tggcgtcatt atggcgggca gcctgaaaat tggtcgttac
    43621 tgcatgatcg gcggagccag cgtaatcaac gggcatatgg aaatatgcga caaagtgacg
    43681 gttacgggca tgggtatggt gatgcgtccc atcactgaac caggcgtcta ttcctcaggc
    43741 attccgctgc aacccaacaa agtctggcgc aaaaccgctg cactggtgat gaacattgat
    43801 gacatgagca agcgtctgaa atcgcttgag cgcaaggtta atcaacaaga ctaacgttcc
    43861 atcttttgtt cgccaaactt tacggcctgt ctcattctta cgattgcggc aggccgtgtt
    43921 attattgtcg tttcttatat tttgacagga agagtatctt gactactaac actcatactc
    43981 tgcagattga agagatttta gaacttctgc cgcaccgttt cccgttctta ctggtggatc
    44041 gcgtgctgga ttttgaagaa ggtcgttttc tgcgcgcagt aaaaaatgtc tctgtcaatg
    44101 agccattctt ccagggccat ttccctggaa aaccgatttt cccgggtgtg ctgattctgg
    44161 aagcaatggc acaggcaaca ggtattctgg cgtttaaaag cgtaggaaaa ctggaaccgg
    44221 gtgagctgta ctacttcgct ggtattgacg aagcgcgctt caagcgcccg gtcgtgcctg
    44281 gcgatcaaat gatcatggaa gtcactttcg aaaaaacgcg ccgcggcctg acccgtttta
    44341 aaggggttgc tctggtcgat ggtaaagtag tttgcgaagc aacgatgatg tgtgctcgta
    44401 gccgggaggc ctgataagtg attgataaat ccgcctttgt gcatccaacc gccattgtgg
    44461 aagagggcgc gtcaattggc gcgaacgcac acattggtcc tttttgtatc gttggacccc
    44521 atgtcgaaat tggtgagggt accgtactga aatctcacgt tgtcgtgaat ggtcatacta
    44581 aaattggccg cgataatgag atttatcagt tcgcctccat cggcgaagtt aaccaggatc
    44641 tgaaatatgc tggcgaaccg acccgtgtgg aaatcggcga tcgtaaccgc attcgcgaaa
    44701 gcgtcaccat tcatcgtggc acagtccagg gcggtggatt gacgaaggtg ggcagcgaca
    44761 acttactgat gatcaacgcg cacattgcgc acgattgtac ggtaggtaac cgctgtattc
    44821 tcgccaacaa cgcaacgctg gcgggtcacg tatcggttga cgacttcgcg atcatcggcg
    44881 gcatgaccgc agtccatcag ttctgcatca ttggtgcgca cgtgatggtt ggcggctgct
    44941 ccggtgtggc gcaggacgtc cctccttatg tcattgcgca gggtaaccac gcaacgccgt
    45001 tcggtgtcaa tatcgaaggg ctgaagcgcc gcggattcag ccgtgaggcg attaccgcta
    45061 tccgcaatgc gtataagctg atttatcgta gcggtaaaac gctcgatgaa gtgaaaccgg
    45121 aaattgctga actggcggaa acatatccgg aagtgaaagc ctttaccgat ttctttgcac
    45181 gctcaacgcg cggtctgatt cgttaatgac tgaacagcgt ccattaacga ttgccctggt
    45241 cgccggagaa acctccggcg atatcctggg ggccggttta atccgcgctc tgaaagaacg
    45301 tgtgcccaac gcccgctttg ttggtgttgc cgggccacga atgcaggctg aaggctgcga
    45361 agcctggtac gaaatggaag aactggcggt gatgggcatt gttgaagtgc tcggtcgtct
    45421 gcgtcgctta ctgcatattc gtgccgatct gacaaagcgt tttggcgaac tgaagccaga
    45481 tatttttgtt ggtattgatg cgcctgactt caatattact cttgaaggta acctcaaaaa
    45541 gcagggtatc aaaaccattc attacgtcag tccatccgtc tgggcgtggc gacagaaacg
    45601 cgttttcaaa ataggcagag ccaccgatat ggtgctcgca tttctgcctt tcgaaaaagc
    45661 gttttatgac aaatacaatg taccgtgccg ctttatcggt cataccatgg ctgatgccat
    45721 gccgttagat ccagataaaa atgccgcccg tgatgtgctg gggatccctc acgatgccca
    45781 ctgcctggcg ttgctaccag ggagccgtgg tgcagaagtt gaaatgctta gtgccgattt
    45841 cctgaaaacg gcccagcttt tgcgccagac atatccggat ctcgaaatcg tggtgccact
    45901 ggtgaatgcc aaacgccgcg agcagtttga acgcatcaaa gctgaagtcg cgccagacct
    45961 ttcagttcat ttgctggatg ggatgggccg tgaggcgatg gtcgccagcg atgcggcgct
    46021 actggcgtcg ggtacggcag ccctggagtg tatgctggcg aaatgcccga tggtggtggg
    46081 atatcgcatg aagcctttta ccttctggtt ggcgaagcgg ctggtgaaaa ctgattatgt
    46141 ctcgctgcca aatctgctgg cgggtagaga gttagtcaaa gaattattgc aggaagagtg
    46201 tgagccgcaa aaactggctg cggcgctgtt accgctgttg gcgaacggga aaaccagcca
    46261 cgcgatgcac gataccttcc gtgaactgca tcagcagatc cgctgcaatg ccgatgagca
    46321 ggcggcacaa gccgttctgg agttagcaca atgatcgaat ttgtttatcc gcacacgcag
    46381 ctggttgcgg gtgtggatga agtcggacgc gggccgttag ttggcgcggt cgtcaccgct
    46441 gcggtgatcc ttgacccggc gcgcccgatt gccgggctga atgattccaa aaagctgagc
    46501 gaaaaacgcc gtctggcgct ctatgaagag atcaaagaga aagcgttgag ctggagtctg
    46561 ggccgcgcgg aaccccacga aatcgacgag ctgaacattc ttcatgcgac catgctggcg
    46621 atgcagcgtg ccgtcgctgg gctgcatatt gcgccggaat atgtgttgat tgatggtaac
    46681 cgctgcccga aattaccgat gcctgcgatg gctgtggtga aaggcgatag ccgcgtaccg
    46741 gaaatcagtg ccgcgtctat cctggcgaaa gtgacgcgtg acgccgaaat ggcggcgctg
    46801 gatattgttt tcccgcaata tggttttgcc caacacaaag ggtacccaac cgcttttcat
    46861 ctggaaaaac tggctgaaca cggcgcgacc gaacaccatc ggcgcagctt tgggcctgtc
    46921 aaacgcgcac tgggacttgc gtcctgattc ttgtgtcgag attaagtaaa ccggaatctg
    46981 aagatgtctg aaccacgttt cgtacacctg cgggtgcaca gcgactactc gatgatcgat
    47041 ggcctggcca aaaccgcacc gttggtaaaa aaggcggcgg cgttgggtat gccagcactg
    47101 gcgatcaccg atttcaccaa cctttgtggt ctggtgaagt tctacggagc gggacatggc
    47161 gcagggatta agcctatcgt cggggcagat tttaacgtcc agtgcgacct gctgggtgat
    47221 gagttaaccc acctgacggt actggcggcg aacaataccg gctatcagaa tctgacgttg
    47281 ctgatctcaa aagcgtatca gcgcgggtac ggtgccgccg ggccgatcat cgatcgcgac
    47341 tggcttatcg aattaaacga agggttgatc cttctttccg gcggacgcat gggcgacgtc
    47401 ggacgcagtc ttttgcgtgg taacagcgcg ctggtagatg agtgtgtcgc gttttatgaa
    47461 gaacacttcc cggatcgcta ttttctcgag ctgatccgca ccggcaggcc ggatgaagaa
    47521 agctatctgc acgcggcggt ggaactggcg gaagcgcgcg gtttgcccgt cgtggcgacc
    47581 aacgacgtgc gctttatcga cagcagcgac tttgacgcac acgaaatccg cgtcgcgatc
    47641 cacgacggct ttaccctcga cgatcctaaa cgcccgcgta actattcgcc gcagcaatat
    47701 atgcgtagcg aagaggagat gtgtgagctg tttgccgaca tccccgaagc ccttgccaac
    47761 accgttgaga tcgccaaacg ctgtaacgta accgtgcgtc ttggtgaata cttcctgccg
    47821 cagttcccga ccggggacat gagcaccgaa gattatctgg tcaagcgtgc aaaagagggc
    47881 ctggaagagc gtctggcctt tttattccct gacgaggaag aacgtgttaa gcgccgcccg
    47941 gaatatgacg aacgtctgga gactgaactt caggttatca accagatggg cttcccgggc
    48001 tacttcctca tcgttatgga atttatccag tggtcgaaag ataacggcgt accggtaggg
    48061 ccaggccgtg gctccggtgc gggttcactg gtggcctacg cgctgaaaat caccgacctc
    48121 gatccgctgg aatttgacct gctgttcgaa cgtttcctta acccggaacg tgtctccatg
    48181 cctgacttcg acgttgactt ctgtatggag aaacgcgatc aggttatcga gcacgtagcg
    48241 gacatgtacg gtcgtgatgc ggtatcgcag atcatcacct tcggtacaat ggcggcgaaa
    48301 gcggtgatcc gcgacgtagg ccgcgtgctg gggcatccgt acggctttgt cgatcgtatc
    48361 tcgaaactga tcccgcccga tccggggatg acgctggcga aagcgtttga agccgagccg
    48421 cagctgccgg aaatctacga agcggatgaa gaagttaagg cgctgatcga catggcgcgc
    48481 aaactggaag gggtcacccg taacgccggt aagcacgccg gtggggtggt tatcgcgccg
    48541 accaaaatta ccgattttgc gccgctttac tgcgatgaag agggcaaaca tccggtcacc
    48601 cagtttgata aaagcgacgt tgaatacgcc ggactggtga agttcgactt ccttggtttg
    48661 cgtacgctca ccatcatcaa ctgggcgctg gagatgatca acaagcggcg ggcgaagaat
    48721 ggcgagccgc cgctggatat cgccgcgatc ccgctggatg acaagaaaag cttcgacatg
    48781 ctgcaacgct cggaaaccac ggcggtattc cagcttgaat cgcgcggcat gaaggacctg
    48841 atcaagcgtc tgcaacctga ctgcttcgaa gatatgatcg cactggtggc gctgttccgc
    48901 ccaggtccgt tgcaatcagg gatggtggat aactttatcg accgtaaaca tggtcgcgaa
    48961 gaaatctcct atccggacgt acagtggcag catgaaagcc tgaaaccggt actggagcca
    49021 acctacggca tcatcctgta tcaggaacag gttatgcaga ttgcgcaggt gctttctggt
    49081 tataccctcg gtggcgcgga tatgctgcgt cgtgcgatgg gtaagaaaaa gccggaagag
    49141 atggctaagc agcgttctgt atttgctgaa ggtgcagaaa agaacggaat caacgccgaa
    49201 ctggcgatga aaatcttcga cctggtggag aaattcgcgg gttacggatt taacaaatcg
    49261 cactctgcgg cctatgcttt ggtgtcatat caaacgttat ggctgaaagc gcactatcct
    49321 gcggagttta tggcggcggt aatgaccgcc gatatggaca acaccgagaa ggtggtgggc
    49381 ctggtggatg agtgctggcg gatggggctg aaaatcctgc caccagatat aaactccggt
    49441 ctttaccatt tccacgtcaa cgacgacggc gaaatcgtgt atggtattgg cgccatcaaa
    49501 ggggtaggtg aaggtccgat tgaggccatc atcgaagccc gtaataaagg cggctacttc
    49561 cgcgaactgt ttgatctttg cgcccgtacc gacaccaaaa agttaaaccg gcgggtgctg
    49621 gaaaaactga tcatgtccgg ggcgtttgac cgtcttggac cacaccgcgc ggcactgatg
    49681 aactcgctgg gcgatgcgtt aaaagcggca gatcaacacg cgaaagcgga agctatcggt
    49741 caggccgata tgttcggcgt gctggccgaa gagccggaac aaattgaaca atcctacgcc
    49801 agctgccaac cgtggccgga gcaggtggta ttagatgggg aacgtgaaac gttaggcctg
    49861 tacctgaccg gacaccctat caaccagtat ttaaaagaga ttgagcgtta tgtcggaggc
    49921 gtaaggctga aagacatgca cccgacagaa cgtggtaaag tcatcacggc tgcggggctc
    49981 gttgttgccg cgcgggttat ggtcaccaag cgcggcaatc gtatcggtat ctgcacgctg
    50041 gatgaccgtt ccgggcggct ggaagtgatg ttgtttactg acgccctgga taaataccag
    50101 caattgctgg aaaaagaccg catacttatc gtcagcggac aggtcagctt tgatgacttc
    50161 agcggtgggc ttaaaatgac cgctcgcgaa gtgatggata ttgacgaagc ccgggaaaaa
    50221 tatgctcgcg ggcttgctat ctcgctgacg gacaggcaaa ttgatgacca gcttttaaac
    50281 cgactccgtc agtctctgga accccaccgc tctgggacaa ttccagtaca tctctactat
    50341 cagagggcgg atgcacgcgc gcggttgcgt tttggcgcga cgtggcgtgt ctctccgagc
    50401 gatcgtttat taaacgatct ccgtggcctc attggttcgg agcaggtgga actggagttt
    50461 gactaataca ggaatactat gagtctgaat ttccttgatt ttgaacagcc gattgcagag
    50521 ctggaagcga aaatcgattc tctgactgcg gttagccgtc aggatgagaa actggatatt
    50581 aacatcgatg aagaagtgca tcgtctgcgt gaaaaaagcg tagaactgac acgtaaaatc
    50641 ttcgccgatc tcggtgcatg gcagattgcg caactggcac gccatccaca gcgtccttat
    50701 accctggatt acgttcgcct ggcatttgat gaatttgacg aactggctgg cgaccgcgcg
    50761 tatgcagacg ataaagctat cgtcggtggt atcgcccgtc ttgacggtcg tccggtgatg
    50821 atcattggtc atcaaaaagg tcgtgaaacc aaagaaaaaa ttcgccgtaa ctttggtatg
    50881 ccagcgccag aaggttaccg caaagcactg cgtctgatgc aaatggctga acgctttaag
    50941 atgcctatca tcacctttat cgacaccccg ggggcttatc ctggcgtggg cgcagaagag
    51001 cgtggtcagt ctgaagccat tgcacgcaac ctgcgtgaaa tgtctcgcct cggcgtaccg
    51061 gtagtttgta cggttatcgg tgaaggtggt tctggcggtg cgctggcgat tggcgtgggc
    51121 gataaagtga atatgctgca atacagcacc tattccgtta tctcgccgga aggttgtgcg
    51181 tccattctgt ggaagagcgc cgacaaagcg ccgctggcgg ctgaagcgat gggtatcatc
    51241 gctccgcgtc tgaaagagct gaaactgatc gactccatca tcccggaacc gctgggcggc
    51301 gctcaccgta acccggaagc gatggcggca tcgttgaaag cgcaactgct ggcggatctg
    51361 gccgatctcg atgtgttaag cactgaagat ttaaaaaatc gtcgttatca gcgcctgatg
    51421 agctacggtt acgcgtaatt cgcaaaagtt ctgaaaaagg gtcacttcgg tggccctttt
    51481 ttatcgccac ggtttgagca ggctatgatt aaggaaggat tttccaggag gaacacatga
    51541 acatcattgc cattatggga ccgcatggcg tcttttataa agatgagccc atcaaagaac
    51601 tggagtcggc gctggtggcg caaggctttc agattatctg gccacaaaac agcgttgatt
    51661 tgctgaaatt tatcgaacat aaccctcgaa tttgcggcgt gatttttgac tgggatgagt
    51721 acagtctcga tttatgcagc gatatcaatc aacttaatga atatctcccg ctttatgcct
    51781 tcatcaacac ccactcgacg atggatgtca gcgtgcagga tatgcggatg gcgctctggt
    51841 tttttgaata tgcgctgggg caggcggaag atatcgccat tcgtatgcgt cagtacaccg
    51901 acgaatatct tgataacatt acaccgccgt tcacgaaagc cttgtttacc tacgttaaag
    51961 agcggaagta caccttttgt acgccggggc atatgggcgg caccgcatat caaaaaagcc
    52021 cggttggctg cctgttttat gattttttcg gcgggaatac cctcaaggct gatgtctcta
    52081 tttcggtcac cgagcttggt tcgttgctcg accacaccgg gccacacctg gaagcggaag
    52141 agtacatcgc gcggactttt ggcgcggaac agagttatat cgttaccaac ggaacatcga
    52201 cgtcgaacaa aattgtgggt atgtacgccg cgccatccgg cagtacgctg ttgatcgacc
    52261 gcaattgtca taaatcgctg gcgcatctgt tgatgatgaa cgatgtagtg ccagtctggc
    52321 tgaaaccgac gcgtaatgcg ttggggattc ttggtgggat cccgcgccgt gaatttactc
    52381 gcgacagcat cgaagagaaa gtcgctgcca ccacgcaggc acaatggccg gttcatgcgg
    52441 tgattaccaa ctccacctat gatggcttgc tctacaacac cgactggatc aaacagacgc
    52501 tggatgtccc gtcgatccac ttcgattccg cctgggtgcc gtacacccat tttcatccga
    52561 tctaccaggg gaaaagtggt atgagcggcg agcgtgttgc gggaaaagtg atcttcgaaa
    52621 cgcaatcgac ccacaaaatg ctggcggcgt tatcgcaggc ttcgctgatc cacattaaag
    52681 gcgagtatga cgaagaggcg tttaacgaag cctttatgat gcataccacc acctcgccca
    52741 gttatcccat tgttgcttcg gttgagacgg cggcggcgat gctgcgtggt aatccgggca
    52801 aacggctgat taaccgttcg gtggagcggg cgctgcattt tcgcaaagag gtccagcggt
    52861 tgcgggaaga gtctgacggt tggtttttcg atatctggca accgccgcag gtggatgaag
    52921 ccgaatgctg gcccgttgcg ccaggcgaac agtggcacgg ctttaacgat gcggatgccg
    52981 atcatatgtt tctcgatccg gttaaagtca ctattttgac accggggatg gacgaacagg
    53041 gcaatatgag cgaggagggg atcccggcag cgctggtggc aaaattcctc gacgaacgtg
    53101 ggatcgtagt agagaaaacc ggcccttata acctgctgtt tctctttagt attggtatcg
    53161 ataaaaccaa agcaatggga ttattgcgtg ggttgacgga atttaaacgc tcttacgatc
    53221 tcaacctgcg gatcaaaaat atgctacccg atctctatgc tgaagatccc gatttctacc
    53281 gcaatatgcg tattcaggat ctggcgcaag ggatccataa gctgattcgt aaacacgatc
    53341 ttcccggttt gatgttgcgg gcattcgata ctttgccgga aatgatcatg acgccacatc
    53401 aggcatggca gagacagatt aaaggtgaag tagaaaccat tgcgctggaa caactggtcg
    53461 gtagagtatc ggcaaatatg atcctgcctt atccaccggg cgtaccgctg ttgatgccag
    53521 gagaaatgct gaccaaagag agccgcacgg tactcgattt tctactgatg ctttgttccg
    53581 tagggcaaca ttaccccggt tttgaaacgg atattcacgg cgcgaaacag gacgaagacg
    53641 gcgtttaccg cgtacgagtc ctaaaaatgg cgggataact tgccagagcg gcttccgggc
    53701 gagtaacgtg ctgttaacaa ataaaggaga cgttatgctg ggtttaaaac aggttcacca
    53761 tattgcgatt attgcgacgg attatgcggt gagcaaagct ttctactgcg atactcttgg
    53821 tttcacgctg caaagcgaag tctatcgcga agcgcgcgac tcgtggaaag gggatttggc
    53881 gcttaatggg caatatgtga ttgagctttt ctcttttccg ttcccgccgg agcgccccag
    53941 ccgaccggaa gcttgcggcc tgcgtcatct ggcttttagc gttgatgaca tcgatgcggc
    54001 agtggcgcac cttgaaagcc ataacgtgaa gtgtgaagcc atccgtgtcg atccatacac
    54061 gcaaaaacgc ttcaccttct ttaacgatcc ggacgggctg ccgttggaac tgtatgagca
    54121 gtaaggcttg tcatcgccgc atttgcccgg taacgtgccg ggcattgcta ctgtaaaatc
    54181 gcaccatcat gacactcacg ctcaatagac aacttctcac ctcacgccag attctggtgg
    54241 cctttagcgg cgggcttgac tccaccgttc tgctgcatca gttggtgcag tggcggacgg
    54301 aaaatccggg cgtcactctg cgcgctattc atgtgcatca cggtttaagt gccaatgccg
    54361 atgcctgggt tacgcattgc gaaaacgtct gccaacagtg gcaggtgccg ctggtggtcg
    54421 aacgcgtaca acttgcgcaa gaggggctgg ggattgaggc ccaagcgcgg caggcgcgtt
    54481 atcaggcatt tgcccgtacc ctattgcccg gcgaagtgct ggtcaccgcg caacatctcg
    54541 acgatcaatg tgaaaccttt ctgctggcgc taaaacgcgg tagcggccct gccgggcttt
    54601 cggctatggg ggaagtctcg gagtttgccg gaacacggct tatccgcccg ttgcttgccc
    54661 gcacgcgggg ggaactggcg cagtgggcat tggcgcatgg tttacgctgg attgaagacg
    54721 aaagtaatca ggacgactca tacgatcgta actttctgcg cctgcgcgta gtgccgttat
    54781 tgcagcagcg ttggccgcat tttgccgaag caacggcccg cagcgccgca ctttgtgctg
    54841 aacaagagag tctgttggat gaactgctgg cggatgattt agcacactgc caaacgtcgc
    54901 aggggacgct gcagattgcg ccaatgctag cgatgagtga tgcccgtcgc gcggcgatta
    54961 tccgccgctg gctggcaggg cagaatgcac cgatgccttc ccgcgacgcg ctggtgcgga
    55021 tctggcagga agtggcgctg gcgcgggaag atgcctcccc ctgtttacgt tttggcgcgt
    55081 ttgaaatacg gcgctatcag tcgcaactgt ggtggattaa ctccgtcact gggcaaagcg
    55141 aaaccattgt gccgtggcag acgtggctac aaccgctgga attaccggcg gggctgggaa
    55201 gtgtacagct tactgcggga ggcgatattc gccctccgcg tgcagacgaa gcggtcagcg
    55261 tgcgtttcaa agcgccagga ttgctgcata ttgtcgggcg taacggcgga cgtaagctaa
    55321 agaaaatctg gcaagagctg ggcgtgccgc cgtggctacg tgacaccacg ccactgctgt
    55381 tttatggcga aacgctgatt gcggcggcag gggtatttgt gacgcaagaa ggtgtggctg
    55441 aaggtgagaa tggcgtaagt tttgtctggc agaaaacgct tagttaagtg aaagccggat
    55501 aagacgcatc aaacgtcgca tccggcgaaa gtcaatcagg actcgctcac caccaccgta
    55561 ccgatttccg ggtggctaaa gctggtaatt ttatccagac gtagctcacg ggtttcgcca
    55621 gcggcctcga cgaccagata ctccacattt ttgcgggaga ctaaatcgct ggcttttgcc
    55681 tgcaattttt cgccatcttt cagctcaagt gtcagcatta aatgatgctg gcaggcgagc
    55741 tcaagattat cgtaatcatc acaattgatt ggttgatacg tatcattcat tgacataatc
    55801 gctcaccagt aagtttgccg cagcgtatgc tgctttttcc ctgacagcct cagaaagggc
    55861 gtcgtcggca gccatttcat tcagcacttt caaaacgcag cccagcgcgt ccggaacgta
    55921 tcctaagtct ccgctggcga tttccgcgta ccgcttgcgt attaactcac aatatttttc
    55981 cacatgccct cctgtcagca ctctgactta accgtggatg caagtctaag cctacgaaga
    56041 taaactctgt ttcgcaaggt gactatacca cactcatttc tgcaatatca gcgccgcaac
    56101 tgcacgtatt ccgttacaat ggcctcctga ttcgaaagga gttttcttat ggcgcttaaa
    56161 gcgacaattt ataaagcgac ggttaatgtg gccgatctcg accgcaacca gtttctcgat
    56221 gcctctctga cgctggcgcg ccacccttca gaaacccagg agcgtatgat gctgcgcttg
    56281 ctggcgtggc tgaaatatgc tgatgaacgt ctgcaattta cccgtggttt gtgtgccgat
    56341 gatgagccgg aagcgtggct gcgtaacgat cacctgggca ttgatttgtg gattgagctg
    56401 gggctgccgg atgagcggcg gattaagaaa gcctgcaccc aggccgcaga agtggtgctg
    56461 tttacctata atagtcgggc ggcgcaaatc tggtggcagc aaaatcagag caaatgtgtg
    56521 cagtttgcca atctttccgt ctggtatctg gacgatgaac aactggcgaa agtaagcgcc
    56581 tttgccgatc gtaccatgac gctgcaggca acgattcagg atggcgtgat ctggttatcg
    56641 gacgataaga ataatctgga agttaactta accgcctggc aacaaccttc atgattgtga
    56701 tttcccgaca tgttgctatt cccgatggtg agcttgagat caccgccatt cgtgcgcagg
    56761 gcgtgggcgg gcagcatgtt aataagacct caacggccat tcatctgcgt tttgacattc
    56821 gggcgtccag cctgccagag tattacaaag agcgtctact cgccgccagc catcatttga
    56881 tcagcagtga tggcgtgatt gtcattaagg cacaggaata ccgcagtcag gaactgaacc
    56941 gcgaagccgc gctagcccgg ctggtggcag tgattaaaga tttaacaaca gaacaaaaag
    57001 cccgacgacc cacgcggccc acccgtgcat cgaaagagcg caggctggca tcgaaagcac
    57061 aaaaatcaag cgtgaaggcg atgcgcggca aagtacgcag cggtcgggaa taaaaagaag
    57121 gaatagatgg tgaaaaaagc gatagtgaca gcgatggctg taatcagcct ctttactctg
    57181 atgggatgta ataatcgggc cgaagttgat gcgctttctc cggcgcaggc tgccgaactg
    57241 aaaccgatgc cgcaaagttg gcgcggcgtg ctgccgtgtg ctgattgcga aggaatcgaa
    57301 acctctctgt tcctcgaaaa agacggaaca tgggtgatga atgagcgtta tctcggtgct
    57361 cgtgaagaac cttcctcctt cgcttcctac ggtacatggg cgcgaaccgc tgacaagctg
    57421 gtattaaccg acagcaaagg tgaaaagtca tattatcggg cgaaaggcga tgcgctggag
    57481 atgctcgatc gtgaaggcaa tccgattgaa tcgcagttca actatacgct ggaaccggca
    57541 caatccagtt tacctatgac gccgatgacc ctgcggggca tgtattttta tatggctgat
    57601 gcggcgacct tcactgattg cgcgaccgga aaacgtttca tggtagcgaa taacgcagag
    57661 ctggagcgtg gctacctggc tgcgcgcggc cacagtgaaa aaccgatgtt actgtcagta
    57721 gaaggtcact ttacgcttga agctaatccg gataccggtg cgccgactaa agtattggca
    57781 cccgatacgg cagggaaatt ttacccaaac caggattgca gtagtttggg gcagtaaacc
    57841 tgcttaaggc agaaacaaac gcaaaattgc ctgatgtgct gcgcttatca ggcctacagc
    57901 atataatgca agattttgaa ttagcacgat tttgtaggcc ggataaggcg tttacgccgc
    57961 atccggcatt ttgtgcacga ggctggcttt atcactgagt cagcccaaca aagcgacccg
    58021 ccttgatata aatcccaggc ttcaggtgcc caacatactg taactgcgtt tctggcttaa
    58081 acgcagagac atcaccaata cgcatatgca tcagatcggc agggctaatc cagcccgatt
    58141 gcgccagtgt aagtggatgc ccggctttgg caaaggcatc cgtgacaaat tccgaacaaa
    58201 accacgactt tttgtctcct tcgcccacac tgcttaactg cgctttcgcc aggccgctga
    58261 cgcactttgg cgaaaatcct cggagaacgg attcagtgag cacatctggc gagtcaccat
    58321 aaagggaata aattcgacaa tgccgcgata gttataaccg ctatctttga ttttattggc
    58381 aaacgcggtg atatctgtgg cttgttgcgg ggtaagatcc gggactcgta agacgaaaag
    58441 cttatcacta tgcttcatcg cttttttaag ggaaacaatc tggacgccag cgcctgtcgc
    58501 ttctgcaacg ttattatcac ccagaaagat tgcaacgtga ctcacagagg aagtgctgaa
    58561 gacgcggatt ccaaatgagg ttaccccaag gcttgaggag aacagcaaat cgccgggttt
    58621 gagatcaggt gccgttattt cttttattga ttgttcggtg aaagagcttt gatgctggaa
    58681 tttgacagcc catgtttttg cctcggcatc tactgctgtg gctgatgggt ctggctggct
    58741 gatatcaacg gtacaagccg aaagtaaaag aaagctgggg agaaacaggc ggcagtacgc
    58801 ctttggttta tccattttat acaatccatg taaaaaaagg gccctgaaat tcaggaccct
    58861 ttctggcatc agcctttaat ctgtttcacc agatattcga cgatgtcacc agtcttaatt
    58921 aactgtttct cgccgttgcg acgatattta tattcgatat cgtcgttgtc gaggttacgg
    58981 tcgcccagca caatagtgtg cggaataccg atcagttcca tatcagcaaa catcacgccc
    59041 ggacgctctt tgcggtcatc cagcagcact tcgatacctt gtgcacgcag ttcgctgtac
    59101 agtttctcag caagctcttg tacgcggaag gatttgtgca tgttcatcgg cagaatcgcc
    59161 acctggaacg gcgcgatagc gtcaggccat acgatgcctc gttcgtcgta gttctgctca
    59221 atcgccgcag ctaccacacg cgttaccccg ataccgtagc aacccatcgt caggatttgg
    59281 ttacggccat cttcaccctg tacggaggct ttcagtgctt cggagtactt ggtacccagc
    59341 tggaagatgt gaccaacttc gataccacgt ttgatcagca gcgtaccctg gccatccggg
    59401 cttggatcgc cagccaccac gttacggata tctgcaactt ccggggtagc gacatcgcga
    59461 tcccagttga tgccgaagta gtgtttacca tcgatgttag caccagcagc gaaatcactc
    59521 atcgccgcaa cggtacggtc aatcaccacc ggaatcggca tgtttaccgg acccagtgaa
    59581 cccggaccgg ctttaaccac ggcacgaatt tcttcttcgg tcgcgaaagt cagcgggctt
    59641 gcaacctgcg gcagtttttc tgctttaact tcgttcagct cgtgatcacc gcgcaccagc
    59701 agcgcaacca gcgggaagct actgccttca accgctttaa ccagcagagt cttaaccgtt
    59761 ttctcaatcg gcagattgaa ctgttcaacc agttccgcga tggttttcgc gttcggcgta
    59821 tcaaccagcg tcatttcctg ggtagcagca gcgcgcggtt ctttcggcgc gatagcttct
    59881 gccagttcaa tgttcgctgc atagtcagag gtgtcggaga agaccacatc gtcttcaccg
    59941 ctctgcgcca gcacctggaa ttcgtgagag gcgctgccgc cgatagaacc ggtgtcggct
    60001 tgtacggcgc ggaaatccag ccccatgcgg ctgaagattt tgctgtaggc cgcatacatt
    60061 gcatcgtagg tttcctgcag ggattcctga gaagtatgga aagagtaagc atctttcatc
    60121 aggaattcgc gggaacgcat gacgccgaaa cgcggacgca cttcgtcgcg gaacttggtc
    60181 tggatctgat agaagttcag cggcagctgt ttgtaagagc taagctcgtt acgaatcagg
    60241 tcagtgataa cttcttcatg agttgggccg agtacgaacg gacgctcgcc acggtcaaca
    60301 aaacgcagca gttccggacc gtactgttcc caacgaccac tctcttgcca caaatcagct
    60361 ggctgaacca ccggcatcga cacctcgatc gcaccggcgt tgttcatctc ttcacgcacg
    60421 atgttttcga cttttttcag aacgcgcacg ccggtcggca gccaggtata taacccggag
    60481 gccagcttgc ggatcatccc ggcgcgcagc atcagctgat ggctgatcac ctcggcgtcg
    60541 gcaggtgtct ccttgagagt ggagagcagg tattggctag tacgcatgtt gttacggttc
    60601 cagttggaag gtagaacagg ctcaaggcga gcctgggaca aaaaaaagtg atttagttta
    60661 ccagtgcaaa agaaatgtca aaagagaagg gcgtgaattt aacgcggttc cagcgcaaag
    60721 acttcaaaac ctgcgtcggt gacgcgccag cgaacgttaa aatcatgtag ccagacggca
    60781 taggttttgc ccgtttcctc acctttacga taggccgggc gtgggtcctg cgccagtact
    60841 tcgcggataa acagcgttaa ctgcggataa cgcttctcca gcgtcaaaag ctgcttttcg
    60901 acctctgcgg taaaactcac cgccatctct gccgctggcg cactttgcgc atagctggca
    60961 ctggcatcgg gaagcgattc ggcaaagggg agatacggtt tgatatccac taccggcgta
    61021 ccatcgacca gatccagact gccgagcttc agaatcacgc tgtctttatg gcaaacaacc
    61081 tctttcagct ctaccagcga cataccaatt gggttagggc ggaaagtaga gcgtgttgcg
    61141 aaaaccccca ttctggcgtt accgccgagg cgcggtggac gcacagtcgg acgccagccg
    61201 ccttccatcg tttgatgaaa gacgaaaagg atccataaat ggctgaacgc ttccaggccg
    61261 cgaacggcgt cggcctggtt gtagggagca atgagatgca gttcgccgtt ggcgcttttt
    61321 accagacctg gctggcgcgg aacggcgaat ttttctttat agggcgagcg aataacgcct
    61381 atttgctcga actggaaact gctcatttcg ccgtaatgtt aagcgcagaa ccgatacata
    61441 cagcctgacg atagcagcct ggcgtaccgc tggtgacttc gcagctatgc agtaataccg
    61501 cattggcctt cattttcgag gcgttgattt gcatccgctt acgtgcggtt ggaatgctcg
    61561 gcggagagtc ctgattggag gcctggcaag aatcgccact gacttcaccg agatcgcgga
    61621 acggtttgcc gactaattct tctgcattgg tataaattcg gaccggcgtg gcgcgcggcg
    61681 ctttcggttt tgcaggctcc gctttcggct ggggtgcagt gctttgaacg ggttcgacag
    61741 gggatctgct taacatggaa cagccgctta gcatgagtgc tactaaacag atcggtaaag
    61801 cacgcatagt atttcctcaa tgtatgatca aaacgtcaat attgaatcag gagcttgtaa
    61861 aaatgacaag acgggcaagc gcccgtcctg aatgatatta caaattgtgg aaacagccta
    61921 aaaattacca gcctttaaca gcgccgccgt taaacacttt gtttgctgct tcgtaaactt
    61981 catcagactg ataagcctgg acgaatttct tcacgttctc ggcgtctttg ttatcttcac
    62041 gcgttacgat caggtttacg tacggggagt ctttatcttc gacaaagata ccgtctttcg
    62101 ctggagtcag gccaatctgg ctggcatagg tggtattgat aactgccaga gcgatttgcg
    62161 cgtcgtccag agatcgaggc agctgcggtg cttccagctc aacaattttc agattttttg
    62221 ggttctcaac aacatcaaga acggtcggca gcaggccaac gccatctttc agtttgatca
    62281 agcccacttt ttgcagcagc agcagtgaac gaccaagatt agttggatcg tttggcacgg
    62341 caacctgcga accatcctgc agttcatcca gtgatttgat tttcttggag taaccagcaa
    62401 tcggataaac aaaagtgttg cctactgcga ccagtttgta gccacgatct ttcagttgct
    62461 gatcaaggta cggtttatgc tggaaggcgt tggcgtcgat atcgcctttg ctcaatgctt
    62521 cgtttggcag aacatagtcg ttgaaggtta ccagctcaac gtccaggcca tatttgtctt
    62581 tcgcaacttt ctgcgcgact tctgctacct gctgttcggc accaacaatc acgccgactt
    62641 taatgtggtt tggatctttt tcatcctgac cgcagcctac cagtgccagt gatccaatca
    62701 gggctcccac tgccgcaaag gttttgaatt tgaacgccat accttattcc ttcttcaatt
    62761 atttatgttg tgttgaacgt tacttgcgag tgacagcccg gacgatgcgg tcgcctgcga
    62821 actgaattaa ataaaccaga atgaccagca ataccagtac cgtattcatc accgtcgcgt
    62881 tatagccgat gtagccatac tgatagccaa tctgacctaa accaccggca ccgactgcac
    62941 cacccatcgc ggaatagccg accagggtaa tcagggtgat agttgccgca ttcaccagac
    63001 ccggcagtgc ttccggtaac agcaccttac ggacgatctg catcggcgtg gcacccattg
    63061 cgcgggaagc ttcaattaac ccggttggga tctccagcag agcgttctcg accatacggg
    63121 caataaacgg tgctgcacca acggttaacg gaacaatcgc tgcctgcaaa ccaatcgatg
    63181 taccgacaat aacgcgggta aacggaatca tccataccag caagataatg aacgggatgg
    63241 aacggaaaat gttcacaatc gcagaaacgg tacgatacag cttcgcgtta gcaataattt
    63301 gccccgggcg cgtaacataa agcaggacgc cgaccggcag accaatcaca aagccaaaaa
    63361 atccggatac gaaggtcatt gccagcgttt cccatacgcc acgaaccagc aaccacatca
    63421 tcggctcaga cataacccag tacctctact tttacatggt gttcctgcag ccaggcaatg
    63481 gcggcttgcg tatcttgttg tgtgccgtgc atttcagtca gcatgatgcc gaacttaacg
    63541 ccaccggcgt aatccatctg cgcgctaata atgttgttgt tgacgttgaa acgacgcgcg
    63601 gtttcagaaa gcagcggggc atcgaccgat tgaccggtaa actccaggcg caacatcggc
    63661 acgcagtcag taaatggctc cgcttgcaga cgttcctggt aatcttccgg gatatccaga
    63721 tgcagggtcg actgaataaa cttctgcgcc agcggcgttt tcggatgcga gaacacttca
    63781 cttaccgtgt cctgctcgat cagttcgcca ttgctgatga ccgccacgca atcacaaata
    63841 cgcttcacaa cgtccatttc gtgggtgatc aacagaatcg tcaaacccag acggcggtta
    63901 atgtctttca gcagttcgag aatagaacgt gtcgttgccg ggtccagcgc gctggtggct
    63961 tcatcacaca gcaatacttt gggattgctg gctaatgcac gggcaatcgc cacacgttgt
    64021 ttctgcccac cggaaagatt cgacgggtag ctatcatgct tatcgccaag accaaccaat
    64081 gacagcaatt ccgtcacgcg acgtttgatc tcgtctttcg gtgtgttgtc cagctccagc
    64141 ggcagagcca cgttgccaaa aacagtacgc gaagagagca ggttaaaatg ctggaaaatc
    64201 ataccaatct ggcggcgagc tttggtcaac tcggattctg acagcgtggt cagttcctgg
    64261 ccatcgacca gcacgctacc ctcggttggg cgctccagca ggtttacaca acgtataagc
    64321 gtactcttac ccgcgcctga ggcaccgata acgccataaa tttgtccagc tggcacatgc
    64381 aggctgacgt tgttcaacgc ctggatggtg cgggtgccct ggtggaacac tttggtgata
    64441 ttcgaaagtt ttatcattga ttatttatta tcgtcattaa gttagtcgtg gcatctcgaa
    64501 tgcctgaaac gggcaacgcc gtcaatgaaa tggatgttaa ggcatccaga cgtctaaatc
    64561 aatcaggttt atgcgaagag cactttcttg caggtcgaaa catgcgatac tagcgtccca
    64621 tgccttatta aggagctata aaaggtggcg aagagcgtac ccgcaatttt tcttgaccgt
    64681 gatggcacca ttaatgtcga tcacggctat gtccatgaga tcgacaactt tgaatttatc
    64741 gacggtgtta ttgacgccat gcgcgagcta aaaaaaatgg gctttgcgct ggtggtagta
    64801 accaaccagt ctggcattgc tcgcggtaaa tttaccgaag cacagtttga aacgctgacc
    64861 gagtggatgg actggtcgct ggcggaccga gatgtcgatc tggatggtat ctattattgc
    64921 ccgcatcatc cgcagggtag tgttgaagag tttcgccagg tctgcgattg ccgcaaacca
    64981 catccgggga tgcttttgtc agcacgcgat tatttgcata ttgatatggc cgcttcttat
    65041 atggtgggcg ataaattaga agatatgcag gcagcggttg cggcgaacgt gggaacaaaa
    65101 gtgctggtgc gtacgggtaa acctattaca cctgaagcag aaaacgcggc agattgggtg
    65161 ttaaatagcc tggcagacct gccgcaagcg ataaaaaagc agcaaaaacc ggcgcaatga
    65221 ttaaaagatg agcggttgaa ataaaaatgc atttttccgc ttgtcttcct gagccgactc
    65281 cctataatgc gcctccatcg acacggcgga tgtgaatcac ttcacacaaa cagccggttc
    65341 ggttgaagag aaaaaatcct gaaattcagg gttgactctg aaagaggaat gtgtaatata
    65401 cgccacctcg cgacagtgag ctgaaagc
//
LOCUS       DAZFQD010000011        65114 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.011, whole genome shotgun sequence.
ACCESSION   DAZFQD010000011 DAZFQD010000000
VERSION     DAZFQD010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 65114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65114
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.011"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            114..677
                     /gene="srlA"
                     /locus_tag="ACHKTW_000776"
                     /gene_synonym="gutA"
     CDS             114..677
                     /gene="srlA"
                     /locus_tag="ACHKTW_000776"
                     /gene_synonym="gutA"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HFO6461846.1"
                     /translation="MIETITHGAEWFIGLFQKGGEVFTGMVTGILPLLISLLVIMNAL
                     INFIGQHRIERFAQRCAGNPVSRYLLLPCIGTFVFCNPMTLSLGRFMPEKYKPSYYAA
                     ASYSCHSMNGLFPHINPGELFVYLGIASGLTTLNLPLGPLAVSYLLVGLVTNFFRGWV
                     TDLTTAIFEKKMGIQLEQKVHLAGATS"
     gene            674..1633
                     /gene="srlE"
                     /locus_tag="ACHKTW_000777"
                     /gene_synonym="gutE"
     CDS             674..1633
                     /gene="srlE"
                     /locus_tag="ACHKTW_000777"
                     /gene_synonym="gutE"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HFO6461847.1"
                     /translation="MTHIRIEKGTGGWGGPLELKATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSGFIYQ"
     gene            1644..2015
                     /gene="srlB"
                     /locus_tag="ACHKTW_000778"
                     /gene_synonym="gutB"
     CDS             1644..2015
                     /gene="srlB"
                     /locus_tag="ACHKTW_000778"
                     /gene_synonym="gutB"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFO6461848.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFSLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            2019..2798
                     /gene="srlD"
                     /locus_tag="ACHKTW_000779"
     CDS             2019..2798
                     /gene="srlD"
                     /locus_tag="ACHKTW_000779"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HFO6461849.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGEGMAYGFGADATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSPMFQSLLPQYATKLGIKPDQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            2903..3262
                     /gene="gutM"
                     /locus_tag="ACHKTW_000780"
     CDS             2903..3262
                     /gene="gutM"
                     /locus_tag="ACHKTW_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HFO6461850.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVGR
                     SSGRFKPRVVVAIALDDQQRIVDTLFMKGLTVFARPQKIPAITGMHAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            3329..4102
                     /gene="srlR"
                     /locus_tag="ACHKTW_000781"
                     /gene_synonym="gutR"
     CDS             3329..4102
                     /gene="srlR"
                     /locus_tag="ACHKTW_000781"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HFO6461851.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFSFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            4095..5060
                     /gene="gutQ"
                     /locus_tag="ACHKTW_000782"
     CDS             4095..5060
                     /gene="gutQ"
                     /locus_tag="ACHKTW_000782"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HFO6461852.1"
                     /translation="MSEALQNAGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTVGGTTL
                     QSQSRAIDAKEILMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(5057..6571)
                     /gene="norR"
                     /locus_tag="ACHKTW_000783"
     CDS             complement(5057..6571)
                     /gene="norR"
                     /locus_tag="ACHKTW_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HFO6461853.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFAGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMMPGDATPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRLGLSRVVLSAGAR
                     NLLQHYRFPGNVRELEHAIHRAVVLSRATRNGDEVILEAQHFAFPEVTLPTPEVAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGMKD"
     gene            6758..8197
                     /gene="norV"
                     /locus_tag="ACHKTW_000784"
     CDS             6758..8197
                     /gene="norV"
                     /locus_tag="ACHKTW_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016966 - nitric oxide reductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HFO6461854.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAVDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSATTTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFEELASEAK"
     gene            8194..9327
                     /gene="norW"
                     /locus_tag="ACHKTW_000785"
     CDS             8194..9327
                     /gene="norW"
                     /locus_tag="ACHKTW_000785"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016731 - oxidoreductase activity, acting
                     on iron-sulfur proteins as donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HFO6461855.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDATIPLTLIAADSMDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAEQFNLHLFPQTWVTDIDAEARVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDRQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNADIYALGDCAE
                     INGQVLPFLQPIQLSAMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFGLLKTLPM"
     gene            complement(9455..11707)
                     /gene="hypF"
                     /locus_tag="ACHKTW_000786"
     CDS             complement(9455..11707)
                     /gene="hypF"
                     /locus_tag="ACHKTW_000786"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016743 - carboxyl- or
                     carbamoyltransferase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HFO6461856.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLLEDPETFLVQLHQHCPPLARIDSVEREPYIWSQLPTEFTIRQSTGGTMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRARKHRPAKPLAVMLPVADGLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPDLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLIHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALVLPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYVV
                     HDVHPGYVSSQWASEMNMPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAATQPWRNLLAQCLRFVPEWQNYSE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCHGVTHPVTMPLVDNQLDLATFWQQWLNWQAPVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(11860..12387)
                     /gene="hydN"
                     /locus_tag="ACHKTW_000787"
     CDS             complement(11860..12387)
                     /gene="hydN"
                     /locus_tag="ACHKTW_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HFO6461857.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVHVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            complement(12536..13546)
                     /gene="ascG"
                     /locus_tag="ACHKTW_000788"
     CDS             complement(12536..13546)
                     /gene="ascG"
                     /locus_tag="ACHKTW_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HFO6461858.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESG
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRKNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSVERLAGYKDALSQHGIALNEKLIA
                     NGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAIKALHERGVAVPEQVSVIGFD
                     DIAIAPYTVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIRRDSLIAL
                     SR"
     gene            13806..15263
                     /gene="ascF"
                     /locus_tag="ACHKTW_000789"
     CDS             13806..15263
                     /gene="ascF"
                     /locus_tag="ACHKTW_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose/arbutin/salicin transporter
                     subunit IIBC"
                     /protein_id="HFO6461859.1"
                     /translation="MAKNYAALARSVIAALGGVDNISAVTHCMTRLRFVIKDDQLIDS
                     PTLKTIPGVLGVVRSDNQCQVIIGNTVSQAFQEVVSLLPGDMQPAQPVGKPKLTLRRI
                     GAGILDALIGTMSPLIPAIIGGSMVKLLAMILEMSGVLTKGSPTLTILNVIGDGAFFF
                     LPLMVAASAAIKFKTNMSLAIAIAGVLVHPSFIELMAKAAQGEHVEFALIPVTAVKYT
                     YTVIPALVMTWCLSYIERWVDRITPAVTKNFLKPMLIVLIAAPLAILLIGPIGIWIGS
                     AISALVYTIHGYLGWLSVAIMGALWPLLVMTGMHRVFTPTIIQTIAETGKEGMVMPSE
                     IGANLSLGGSSLAVAWKTKNPELRQTALAAAASAIMAGISEPALYGVAIRLKRPLIAS
                     LISGFICGAVAGMAGLASHSMAAPGLFTSVQFFDPANPMSIVWVFAVMALAVVLSFIL
                     TLLLGFEDIPVEEAAAEARKHQSAQPTVAKEVSLN"
     gene            15272..16696
                     /gene="ascB"
                     /locus_tag="ACHKTW_000790"
     CDS             15272..16696
                     /gene="ascB"
                     /locus_tag="ACHKTW_000790"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase AscB"
                     /protein_id="HFO6461860.1"
                     /translation="MSVFPEGFLWGGALAANQSEGAFREGGKGLTTVDMIPHGEHRMA
                     VKLGLEKRFQLRDDEFYPSHEATDFYHRYKEDIALMAEMGFKVFRTSIAWSRLFPQGD
                     EITPNQQGIAFYRSVFEECKKYGIEPLVTLCHFDVPMHLVTEYGSWRNRKLVEFFSRY
                     ARTCFEAFDGLVKYWLTFNEINIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLVASA
                     LATKIAHEVNPQNQVGCMLAGGNFYPYSCKPEDVWAALEKDRENLFFIDVQARGAYPA
                     YSARVFREKGVTINKAPGDDEILKNTVDFVSFSYYASRCASAEMNANNSSAANVVKSL
                     RNPYLQVSDWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAKDELAANGEINDDYR
                     ISYLREHIRAMGEAIADGIPLMGYTTWGCIDLVSASTGEMSKRYGFVYVDRDDAGNGT
                     LTRTRKKSFWWYKKVIASNGEDLE"
     gene            complement(16810..17229)
                     /locus_tag="ACHKTW_000791"
     CDS             complement(16810..17229)
                     /locus_tag="ACHKTW_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO6461861.1"
                     /translation="MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEE
                     DDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPVGVALLRTLIDQHNLTYADLKNE
                     IGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL"
     gene            complement(17300..18055)
                     /locus_tag="ACHKTW_000792"
     CDS             complement(17300..18055)
                     /locus_tag="ACHKTW_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6461862.1"
                     /translation="MCAFLLSLVLPAQATSFTEYLPMSDSEYAQKRALKPLLTMPYDA
                     EQNWHFRKVGVAGVTLEKMPNDDSEWQLNGKDRAGKSWSVPVGVLQNMAGNAQLYRAD
                     LDRNGIQDLVIWRGISGNGLAPNAFLILMTFNQQGRPCVFQSDGFYTASETGIDDLLD
                     LQRNGHTQLLDMQFDSGYWITSLYQVKDAKWQRVHGWFGKLSYPALTRFTYTPNRKLV
                     LKPIAGRDPQTEDLAQTQRCLIKGDVLYGVNQN"
     gene            complement(18098..18568)
                     /gene="hycI"
                     /locus_tag="ACHKTW_000793"
     CDS             complement(18098..18568)
                     /gene="hycI"
                     /locus_tag="ACHKTW_000793"
                     /EC_number="3.4.23.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation peptidase HycI"
                     /protein_id="HFO6461863.1"
                     /translation="MTDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPE
                     NDIVAIRELRPTRLLIVDATDMGLNPGEIRIIDPDDIAEMFMMTTHNMPLNYLIDQLK
                     EDIGEVIFLGIQPDIVGFYYPMTQPIKDAVETVYQRLEGWEGNGGFAQLAVEVE"
     gene            complement(18561..18971)
                     /gene="hycH"
                     /locus_tag="ACHKTW_000794"
     CDS             complement(18561..18971)
                     /gene="hycH"
                     /locus_tag="ACHKTW_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase assembly protein HycH"
                     /protein_id="HFO6461864.1"
                     /translation="MSEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDC
                     LEAALTCPWDEYLAWIATLEAGSEARRKMEGVPKYGEIVIDINHVPMLANAFDKARAA
                     QTSQQQEWSTMLLSMLHDIHQENAIYLMVRRLRD"
     gene            complement(18968..19735)
                     /gene="hycG"
                     /locus_tag="ACHKTW_000795"
     CDS             complement(18968..19735)
                     /gene="hycG"
                     /locus_tag="ACHKTW_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycG"
                     /protein_id="HFO6461865.1"
                     /translation="MSNLLGPRDANGIPVPMTVDESIASMKASLLKKIKRSAYVYRVD
                     CGGCNGCEIEIFATLSPLFDAERFGIKVVPSPRHADILLFTGAVTRAMRSPALRAWQS
                     APDPKICISYGACGNSGGIFHDLYCVWGGTDKIVPVDVYIPGCPPTPAATLYGFAMAL
                     GLLEQKIHARGPGEQDEQPAEILHGDMVQPLRVKVDREARRLAGYRYGRQIADDFLTQ
                     LGQGEEQVARWLEAENDPRLNEIVSHLNHVVEEARIR"
     gene            complement(19735..20277)
                     /gene="hycF"
                     /locus_tag="ACHKTW_000796"
     CDS             complement(19735..20277)
                     /gene="hycF"
                     /locus_tag="ACHKTW_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycF"
                     /protein_id="HFO6461866.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAVDKNFRGKPEQNPQQCIGCA
                     ACVNACPSNALTVETDLATGELAWEFNLGRCIFCGRCEEVCPTAAIKLSQEYELAVWK
                     KEDFLQQSRFVLCSCRVCNRPFAVQKEIDYAIALLKHNGDSRAENHRESFETCPECKR
                     QKCLVPSDRIELTRHMKEAI"
     gene            complement(20287..21996)
                     /gene="hycE"
                     /locus_tag="ACHKTW_000797"
     CDS             complement(20287..21996)
                     /gene="hycE"
                     /locus_tag="ACHKTW_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HFO6461867.1"
                     /translation="MSEEKLGQHYLAALNEAFPGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
                     PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHR
                     GMEKLAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILL
                     EVERLHSHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIR
                     RDLLKDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGP
                     MVRASGHARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLD
                     NLPGGPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPT
                     LRYMLRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNS
                     PLK"
     gene            complement(22014..22937)
                     /gene="hycD"
                     /locus_tag="ACHKTW_000798"
     CDS             complement(22014..22937)
                     /gene="hycD"
                     /locus_tag="ACHKTW_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HFO6461868.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIIKLLGRQSVGPDASGWVFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTAGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWAGFGFAFLAFVSLLAA"
     gene            complement(22940..24766)
                     /gene="hycC"
                     /locus_tag="ACHKTW_000799"
     CDS             complement(22940..24766)
                     /gene="hycC"
                     /locus_tag="ACHKTW_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HFO6461869.1"
                     /translation="MSAISLINSGVAWFVAAAVLAFLFSFQKTLSGWIAGIGGAVGSL
                     YTAAAGFTVLTGAVGVSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHA
                     QVKCNGLQINMLMAAAVCAVIASNLGMFVVMAEVMALCAVFLTSNSKEGKLWFALGRL
                     GTLLLAIACWLLWQRYGTLDLRLLDMRMQQLPLGSDIWLLGVIGFGLLAGIIPLHGWV
                     PQAHANASAPAAALFSTVVMKIGLLGILTLSLLGGNAPLWWGIALLVLGMITAFVGGL
                     YALMEHNIQRLLAYHTLENIGIILLGLGAGVTGIALEQPALIALGLVGGLYHLLNHSL
                     FKSVLFLGAGSVWFRTGHRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEW
                     VIYQSFFKLSNSGAFVARLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAEN
                     ATCAPLLMSVSVVALAICCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLI
                     ACPLLPFIIMAICKGDRLPSRSRGSAWVCGYDHEKSMVITAHGFAMPVKQAFAPVLKL
                     RKWLNPVSLVPGWQCEGSALLFRRMALVELAVLVVIIVSRGA"
     gene            complement(24763..25374)
                     /gene="hycB"
                     /locus_tag="ACHKTW_000800"
     CDS             complement(24763..25374)
                     /gene="hycB"
                     /locus_tag="ACHKTW_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HFO6461870.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARISTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            complement(25499..25960)
                     /gene="hycA"
                     /locus_tag="ACHKTW_000801"
     CDS             complement(25499..25960)
                     /gene="hycA"
                     /locus_tag="ACHKTW_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HFO6461871.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYYNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            26172..26522
                     /gene="hypA"
                     /locus_tag="ACHKTW_000802"
     CDS             26172..26522
                     /gene="hypA"
                     /locus_tag="ACHKTW_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HFO6461872.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            26526..27398
                     /gene="hypB"
                     /locus_tag="ACHKTW_000803"
     CDS             26526..27398
                     /gene="hypB"
                     /locus_tag="ACHKTW_000803"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HFO6461873.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPKMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHAPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIAYAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            27389..27661
                     /gene="hypC"
                     /locus_tag="ACHKTW_000804"
     CDS             27389..27661
                     /gene="hypC"
                     /locus_tag="ACHKTW_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HFO6461874.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            27661..28782
                     /gene="hypD"
                     /locus_tag="ACHKTW_000805"
     CDS             27661..28782
                     /gene="hypD"
                     /locus_tag="ACHKTW_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HFO6461875.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQENEA"
     gene            28779..29789
                     /gene="hypE"
                     /locus_tag="ACHKTW_000806"
     CDS             28779..29789
                     /gene="hypE"
                     /locus_tag="ACHKTW_000806"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HFO6461876.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAVSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARTAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDMLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISEAALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            29863..31941
                     /gene="flhA"
                     /locus_tag="ACHKTW_000807"
     CDS             29863..31941
                     /gene="flhA"
                     /locus_tag="ACHKTW_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
                     /protein_id="HFO6461877.1"
                     /translation="MSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALA
                     DNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLAHGPVRSILSRPDTLHCSYEEFC
                     ETWPQLAAGGLYPKFGHYCLMPLAAEGHIFGGCEFIRYDDRPWSEKEFNRLQTFTQIV
                     SVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYF
                     DIDDISIVLRSHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINL
                     HERDDLAPYERMLFDTWGNQIQTLCLLPLMSGDTMLGVLKLAQCEEKVFTTTNLNLLR
                     QIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVL
                     KQVEMVAQSDSTVLILGETGTGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDL
                     FGHERGAFTGASAQRIGRFELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSN
                     KIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIPLLAKAF
                     TFKIARRLGRNIDSIPAETLRTLSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDI
                     VLPEPETPPAATVVAQEGEDEYQLIVRVLKETNGVVAGPKGAAQRLGLKRTTLLSRMK
                     RLGIDKSALI"
     gene            complement(31978..32331)
                     /gene="ygbA"
                     /locus_tag="ACHKTW_000808"
     CDS             complement(31978..32331)
                     /gene="ygbA"
                     /locus_tag="ACHKTW_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HFO6461878.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKKPRE"
     gene            complement(32363..32542)
                     /locus_tag="ACHKTW_000809"
     CDS             complement(32363..32542)
                     /locus_tag="ACHKTW_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001363568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6461879.1"
                     /translation="MIPELIISGEEANGEFLMLVPGKMLFCSFCVMLMKQVRKIASVG
                     EPFFFRVIVPFKIKM"
     gene            32618..35179
                     /gene="mutS"
                     /locus_tag="ACHKTW_000810"
     CDS             32618..35179
                     /gene="mutS"
                     /locus_tag="ACHKTW_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFO6461880.1"
                     /translation="MSAIENFDAHTPMMQQYLRLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRTNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            35285..35941
                     /gene="pphB"
                     /locus_tag="ACHKTW_000811"
     CDS             35285..35941
                     /gene="pphB"
                     /locus_tag="ACHKTW_000811"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HFO6461881.1"
                     /translation="MPSTRYQKINAHHYRHIWVVGDIHGEYQLLQSRLHQLSFYPETD
                     LLISTGDNIDRGPKSLNVLRLLNQPWFTSVKGNHEAMALDAFETGDGNMWLASGGDWF
                     FDLNDSEQQEAIDLLLKFHHLPHIIEITNDNIKYVIAHADYPGSEYLFGKEIAESELL
                     WPVDRVQKSLNGELQQINGADYFIFGHMMFDNIQTFANQIYIDTGTPKSGRLSFYKIK
                     "
     gene            complement(35992..36759)
                     /gene="ygbI"
                     /locus_tag="ACHKTW_000812"
     CDS             complement(35992..36759)
                     /gene="ygbI"
                     /locus_tag="ACHKTW_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor YgbI"
                     /protein_id="HFO6461882.1"
                     /translation="MIPVERRQIILEMVAEKGIVSIAELTDRMNVSHMTIRRDLQKLE
                     QQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVQPGSCIYLDAGT
                     TTLAIAQHLIHMESLTVVTNDFVIANYLLDNSNCTIIHTGGAVCRENRSCVGEAAATM
                     LRSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDATKYGQVATWL
                     ALPLSEFDQIITDDGLPESASLALAKQDLSLLVAKNE"
     gene            36955..37863
                     /gene="ltnD"
                     /locus_tag="ACHKTW_000813"
     CDS             36955..37863
                     /gene="ltnD"
                     /locus_tag="ACHKTW_000813"
                     /EC_number="1.1.1.411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonate dehydrogenase"
                     /protein_id="HFO6461883.1"
                     /translation="MKTGSEFHVGIVGLGSMGMGAALSCVRAGLSTWGADLNSNACAT
                     LKEAGACGVSDNAATFAEKLDALLVLVVNAAQVKQVLFGETGVAQHLKPGTAVMVSST
                     IASADAQEIATALAGFDLEMLDAPVSGGAVKAANGEMTVMASGSDIAFERLAPVLEAV
                     AGKVYRIGAEPGLGSTVKIIHQLLAGVHIAAGAEAMALAARAGIPLDVMYDVVTNAAG
                     NSWMFENRMRHVVDGDYTPHSAVDIFVKDLGLVADTAKALHFPLPLASTALNMFTSAS
                     NAGYGKEDDSAVIKIFSGITLPGAKS"
     gene            37860..39122
                     /gene="otnK"
                     /locus_tag="ACHKTW_000814"
     CDS             37860..39122
                     /gene="otnK"
                     /locus_tag="ACHKTW_000814"
                     /EC_number="2.7.1.217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxo-tetronate kinase"
                     /protein_id="HFO6461884.1"
                     /translation="MIKIGVIADDFTGATDIASFLVENGLPTVQINGVPTGKMPEAID
                     ALVISLKTRSCPVVEATQQSLAALSWLQQQGCKQIYFKYCSTFDSTAKGNIGPVTDAL
                     MDALDTPFTVFSPALPVNGRTVYQGYLFVMNQLLAESGMRHHPVNPMTDSYLPRLVEA
                     QSTGRCGVVSAHVFEQGVDAVRQELARLQQEGYRYAVLDALTEHHLEIQGEALRDAPL
                     VTGGSGLAIGLARQWAQENGNQAREAGHPLAGRGVVLSGSCSQMTNRQVAHYRQIAPA
                     REVDVARCLSTETLAAYAHELAEWVLGQESVLAPLVFATASTDALAAIQQQYGAQKAS
                     QAVETLFSQLAARLAAEGVTRFIVAGGETSGVVTQSLGIKGFHIGPTISPGVPWVNAL
                     DKPVSLALKSGNFGDEAFFSRAQREFLS"
     gene            39119..39757
                     /gene="ygbL"
                     /locus_tag="ACHKTW_000815"
     CDS             39119..39757
                     /gene="ygbL"
                     /locus_tag="ACHKTW_000815"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HFO6461885.1"
                     /translation="MSDFAKVEQSLREEMTRIASSFFQRGYATGSAGNLSLLLPDGNL
                     LATPTGSCLGNLDPQRLSKVAADGEWLSGDKPSKEVLFHLALYRNNPRCKAVVHLHST
                     WSTALSCLQGLDSSNVIRPFTPYVVMRMGNVPLVPYYRPGDKRIAQDLAELAADNQAF
                     LLANHGPVVCGESLQEAANNMEELEETAKLIFILGDRPIRYLTAGEIAELRS"
     gene            39762..40538
                     /gene="ygbM"
                     /locus_tag="ACHKTW_000816"
     CDS             39762..40538
                     /gene="ygbM"
                     /locus_tag="ACHKTW_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HFO6461886.1"
                     /translation="MPRFAANLSMMFTEVPFIERFAAARKAGFDAVEFLFPYDYSTLQ
                     IQKQLEQNHLTLALFNTAPGDINAGEWGLSALPGREHEAHADIDLALEYALALNCEQV
                     HVMAGVVPAGEDAERYRAVFIDNLRYAADRFALHGKRILVEALSPDVKPHYLFSSQYQ
                     ALAIVEEVARDNVFIQLDTFHAQKVDGNLTHLIRDYAGKYAHVQIAGLPDRHEPDDGE
                     INYPWLFRLFDEVGYQGWIGCEYKPRGLTEEGLGWFDAWR"
     gene            40627..41991
                     /gene="ygbN"
                     /locus_tag="ACHKTW_000817"
     CDS             40627..41991
                     /gene="ygbN"
                     /locus_tag="ACHKTW_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015128 - gluconate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035429 - gluconate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family transporter"
                     /protein_id="HFO6461887.1"
                     /translation="MSTITLLCIALAGVIMLLLLVIKAKVQPFVALLLVSLLVALAAG
                     IPAGEVGKVMIAGMGGVLGSVTIIIGLGAMLGRMIEHSGGAESLANYFSRKLGDKRTI
                     AALTLAAFFLGIPVFFDVGFIILAPIIYGFAKVAKISPLKFGLPVAGIMLTVHVAVPP
                     HPGPVAAAGLLHADIGWLTIIGIAISIPVGIVGYFAAKIINTRQYAMSVEVLEQMQLA
                     PASEEGATKLSDKINPPGVALVTSLIVIPIAIIMAGTVSATLMPPSHPLLGTLQLIGS
                     PMVALMIALVLAFWLLALRRGWSLQHTSDIMGSALPTAAVVILVTGAGGVFGKVLVES
                     GVGKALANMLQMIDLPLLPAAFIISLALRASQGSATVAILTTGGLLSEAVMGLNPIQC
                     VLVTLAACFGGLGASHINDSGFWIVTKYLGLSVADGLKTWTVLTTILGFTGFLITWCV
                     WAVI"
     gene            complement(42030..42266)
                     /locus_tag="ACHKTW_000818"
     CDS             complement(42030..42266)
                     /locus_tag="ACHKTW_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit D"
                     /protein_id="HFO6461888.1"
                     /translation="MICPRCADEQIEVMAKSPVKDVWTVYQCQHCLYTWRDTEPLRRT
                     SREHYPEAFRMTQKDIDDAPMVPSIPPLLVEGKR"
     gene            complement(42277..43704)
                     /locus_tag="ACHKTW_000819"
     CDS             complement(42277..43704)
                     /locus_tag="ACHKTW_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit C"
                     /protein_id="HFO6461889.1"
                     /translation="MAFDDLRSFLQALDDHGQLLKISEEVNAEPDLAAAANATGRIGD
                     GAPALWFDNIRGFTDARVAMNTIGSWQNHAISLGLPPNAPVKKQIDEFIRRWDNFPIA
                     PERRANPAWAQNTVDGDEINLFDILPLFRLNDGDGGFYLDKACVVSRDPLDPDNFGKQ
                     NVGIYRMEVKGKRKLGLQPVPMHDIALHLHKAEERGEDLPIAITLGNDPIITLMGATP
                     LKYDQSEYEMAGALRESPYPIATAPLTGFDVPWGSEVILEGVIESRKREIEGPFGEFT
                     GHYSGGRNMTVVRIDKVSYRTRPIFESLYLGMPWTEIDYLMGPATCVPLYQQLKAEFP
                     EVQAVNAMYTHGLLAIISTKKRYGGFARAVGLRAMTTPHGLGYVKMVIMVDEDVDPFN
                     LPQVMWALSSKVNPAGDLVQLPNMSVLELDPGSSPAGITDKLIIDATTPVAPDNRGHY
                     SQPVVDLPETKAWAEKLTAMLAARK"
     gene            complement(43704..44297)
                     /locus_tag="ACHKTW_000820"
     CDS             complement(43704..44297)
                     /locus_tag="ACHKTW_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit B"
                     /protein_id="HFO6461890.1"
                     /translation="MKLIVGMTGATGAPLGVALLQALREMPNVETHLVMSKWAKTTIE
                     LETPYSARDVAALADFSHNPADQAAIISSGSFRTDGMIVIPCSMKTLAGIRAGYADGL
                     VGRAADVVLKEGRKLVLVPREMPLSTIHLENMLALSRMGVAMVPPMPAFYNHPETVDD
                     IVHHVVARVLDQFGLEHPHARRWQGLPQARNFSQENE"
     gene            44444..44851
                     /locus_tag="ACHKTW_000821"
     CDS             44444..44851
                     /locus_tag="ACHKTW_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001208089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFO6461891.1"
                     /translation="MALRNKAFHQLRQLFQQHTARWQHELPDLTKPQYAVMRAIADKP
                     GIEQVALIEAAVSTKATLAEMLARMENRGLVRREHDPADKRRRFVWLTAEGEKILAAA
                     IPIGDSVDEEFLGRLSGEEQELFMQLVRKMMNA"
     gene            complement(44972..45964)
                     /gene="rpoS"
                     /locus_tag="ACHKTW_000822"
     CDS             complement(44972..45964)
                     /gene="rpoS"
                     /locus_tag="ACHKTW_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HFO6461892.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRE"
     gene            complement(46027..47121)
                     /gene="nlpD"
                     /locus_tag="ACHKTW_000823"
     CDS             complement(46027..47121)
                     /gene="nlpD"
                     /locus_tag="ACHKTW_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HFO6461893.1"
                     /translation="MSLVSLWLAGCSDTSNPPAPVSSVNGNAPANTNSGMLITPPPKM
                     GTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENGRIVYNRQYGNIPKGS
                     YSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVGQTLQVGNASGTPITG
                     GNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSANKMLPNNKPAATTVT
                     APVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASEGGNKGIDIAGSKGQA
                     IIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLVREQQEVKAGQKIATM
                     GSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            complement(47306..47932)
                     /gene="pcm"
                     /locus_tag="ACHKTW_000824"
     CDS             complement(47306..47932)
                     /gene="pcm"
                     /locus_tag="ACHKTW_000824"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HFO6461894.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            complement(47926..48687)
                     /gene="surE"
                     /locus_tag="ACHKTW_000825"
     CDS             complement(47926..48687)
                     /gene="surE"
                     /locus_tag="ACHKTW_000825"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HFO6461895.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     PDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            complement(48668..49717)
                     /gene="truD"
                     /locus_tag="ACHKTW_000826"
     CDS             complement(48668..49717)
                     /gene="truD"
                     /locus_tag="ACHKTW_000826"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HFO6461896.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHAREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGAQRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEQ
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            complement(49714..50193)
                     /gene="ispF"
                     /locus_tag="ACHKTW_000827"
     CDS             complement(49714..50193)
                     /gene="ispF"
                     /locus_tag="ACHKTW_000827"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HFO6461897.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            complement(50193..50903)
                     /gene="ispD"
                     /locus_tag="ACHKTW_000828"
     CDS             complement(50193..50903)
                     /gene="ispD"
                     /locus_tag="ACHKTW_000828"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HFO6461898.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVKRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDAQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            complement(50922..51233)
                     /gene="ftsB"
                     /locus_tag="ACHKTW_000829"
     CDS             complement(50922..51233)
                     /gene="ftsB"
                     /locus_tag="ACHKTW_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HFO6461899.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAAQQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            complement(51427..51750)
                     /gene="ygbE"
                     /locus_tag="ACHKTW_000830"
     CDS             complement(51427..51750)
                     /gene="ygbE"
                     /locus_tag="ACHKTW_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HFO6461900.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFIYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGALF
                     MWLLG"
     gene            complement(51800..52405)
                     /gene="cysC"
                     /locus_tag="ACHKTW_000831"
     CDS             complement(51800..52405)
                     /gene="cysC"
                     /locus_tag="ACHKTW_000831"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HFO6461901.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLGVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            complement(52405..53832)
                     /gene="cysN"
                     /locus_tag="ACHKTW_000832"
     CDS             complement(52405..53832)
                     /gene="cysN"
                     /locus_tag="ACHKTW_000832"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HFO6461902.1"
                     /translation="MNTALAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIRKDYLTFAEQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGPTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGTLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEAFAVEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAAPSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            complement(53834..54742)
                     /gene="cysD"
                     /locus_tag="ACHKTW_000833"
     CDS             complement(53834..54742)
                     /gene="cysD"
                     /locus_tag="ACHKTW_000833"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HFO6461903.1"
                     /translation="MDQKRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            54994..56031
                     /gene="iap"
                     /locus_tag="ACHKTW_000834"
     CDS             54994..56031
                     /gene="iap"
                     /locus_tag="ACHKTW_000834"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HFO6461904.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKNWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGMDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTAAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     repeat_region   56113..56568
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=56113..56141
                     /rpt_unit_seq="cggtttatccccgctggcgcggggaacac"
     gene            complement(56665..56958)
                     /gene="cas2e"
                     /locus_tag="ACHKTW_000835"
     CDS             complement(56665..56958)
                     /gene="cas2e"
                     /locus_tag="ACHKTW_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endoribonuclease
                     Cas2e"
                     /protein_id="HFO6461905.1"
                     /translation="MSMVVVVTENVPPRLRGRLAIWLLEVRAGVYVGDTSKRIREMIW
                     QQITQLAGCGNVVMAWATNTESGFEFQTWGENRRIPVDLDGLRLVSFLPVDNQ"
     gene            complement(56958..57878)
                     /gene="cas1e"
                     /locus_tag="ACHKTW_000836"
     CDS             complement(56958..57878)
                     /gene="cas1e"
                     /locus_tag="ACHKTW_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004520 - DNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endonuclease Cas1e"
                     /protein_id="HFO6461906.1"
                     /translation="MTFVPLSPIPLKDRTSMIFLQYGQIDVLDGAFVLIDKTGIRTHI
                     PVGSVACIMLEPGTRVSHAAVHLAATVGTLLVWVGEAGVRVYSSGQPGGARADKLLYQ
                     AKLALTEDLRLKVVRKMYELRFREPQPARRSVEQLRGIEGSRVRQTYALLAKQYGVKW
                     NGRKYDPKDWEKGDVVNRCISAATSCLYGISEAAVLAAGYAPAIGFIHSGKPLSFVYD
                     IADIIKFDSVVPKAFEIAARQPAEPDKEVRLACRDIFRSTKLTGKLIPLIEEVLAAGE
                     IEPPQPAPDMLPPAIPEPETLGDSGHRGRG"
     gene            complement(57875..58525)
                     /gene="cas6e"
                     /locus_tag="ACHKTW_000837"
     CDS             complement(57875..58525)
                     /gene="cas6e"
                     /locus_tag="ACHKTW_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
                     /protein_id="HFO6461907.1"
                     /translation="MYLSRITLHTGQLSPAQLLHLVDRGEYVMHQWLWDLFPGGKERQ
                     FLYRREELQGAFRFFVLSQERPAESDTFTIECRSFAPELCTGQQLCFNLRANPTICKA
                     GKRHDLLMEAKRQVRGQAEGSDVWLHQQQAALDWLAAQGERSGFTLLDTSVDAYRQQQ
                     LRRENSRQLIQFSSVDYTGMLTVTDPGLFVQRLSQGYGKSRAFGCGLMLIKPGAEA"
     gene            complement(58507..59253)
                     /gene="cas5e"
                     /locus_tag="ACHKTW_000838"
     CDS             complement(58507..59253)
                     /gene="cas5e"
                     /locus_tag="ACHKTW_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cas5/CasD"
                     /protein_id="HFO6461908.1"
                     /translation="MSQYLIFQLHGPMASWGVDAPGEVRHTHELPSRSALLGLLAAGV
                     GIRRDDTERLNAFNRHYSLVVCASRNPRWARDYHTVQMPKEVRKARYFSRREELSDPE
                     LLSAIISRRDYYTDAWWMVAVATTADAPYSLEQLQDGLRHPVFPLYLGRKSHPLALPL
                     APLLLEGNACDALCNAYQQYQDHFHKLKVSLPKLQDECWWEGKHDGLVASKILRRRDV
                     PLNRQQWLFGERTVNQGPWLSKEEPCTSQE"
     gene            complement(59264..60337)
                     /gene="cas7e"
                     /locus_tag="ACHKTW_000839"
     CDS             complement(59264..60337)
                     /gene="cas7e"
                     /locus_tag="ACHKTW_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas7/Cse4/CasC"
                     /protein_id="HFO6461909.1"
                     /translation="MTTFIQLHLLTAYPAANLNRDDSGSPKTVILGGATRLRVSSQSL
                     KRAWRTSELFEQALAGHIGVRSGRIAREAATILIEKGIEDKKAIEWAVEIADYLGKAK
                     KDKKQKNDKKPKDPLTSAETEQLVHISPAEFDAVKALAHQLAEEKRAPKEKDLALLRK
                     DRMAVDIAMFGRMLAKKPEFNVEAACQVAHAFGVSETIVENDFFTAVDDLRQASEDAG
                     AGHVDETGFGSALFYTYICIDKDLLVENLGGDEALANQTIRAFTEAALKVSPTGKQNS
                     FASRAYASWALAEKGTDQPRSLAAAFYEPINGTRQLDVAVQRITTLRENMNTVYEQKT
                     ECASFDVMNKQGSMKDVLDFICA"
     gene            complement(60352..60885)
                     /gene="casB"
                     /locus_tag="ACHKTW_000840"
                     /gene_synonym="cse2"
     CDS             complement(60352..60885)
                     /gene="casB"
                     /locus_tag="ACHKTW_000840"
                     /gene_synonym="cse2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000029312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse2/CasB"
                     /protein_id="HFO6461910.1"
                     /translation="MSIVKDEHKVALRKWHEELQDKRGERASLRRSTTVNDVCLTDGF
                     RLFLKNRQIKWQDEPEWRITALALIAAVSANVKAIDERQPFAAQLAAVMKKGRFTRLL
                     AVKTPDDLLRQLRRAVKLLNGSVNLDSLAEGVFRWCQESDDLLNHHRRQQRPTEFIRI
                     RWALEYYQAGDADNEQN"
     gene            complement(60882..62444)
                     /gene="casA"
                     /locus_tag="ACHKTW_000841"
                     /gene_synonym="cse1"
     CDS             complement(60882..62444)
                     /gene="casA"
                     /locus_tag="ACHKTW_000841"
                     /gene_synonym="cse1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse1/CasA"
                     /protein_id="HFO6461911.1"
                     /translation="MNSFSLLTTPWLPVRFKDGTTGKLAPVDLADENVVDIAAPRADL
                     QGAAWQFLLGLLQTSFAPKNHGRWDDIWQDGLEAEKLREALLSLEHAFQFGPDSPSFM
                     QDFDELKVKATSIASLLPDAPGKQTKERNTDHFIKRDTTQHLCLHCVPLALFSIQLNA
                     PIGGRGYYPGLRGGGPLTTLIELLEYQGNQQTPLWRKLWLNVMPQDEADLPLPKTFDD
                     LVFPWLAPTRTSELDGAVVTDEQVNKLQAYWGMPRRIRIDFKTTSIGNCDICGRQSDA
                     LLGLMSLKNYGVQYVMWRHPLTPYRLPLKEGGDFYSVKPQPGGLIWRDWLGLIEVGNS
                     KNNTELPAQVVKLLNASNLKQTRVGLWGFGFDFEDMKVRCWYEHHFPLLLNKKEDLIP
                     KLRLAAQAASHILSLLHRALKEAWFSEKKTTKLDFGFVDIDFWNKTQHRFLRLVRKIE
                     EGQDPYELLSKWQKEMWLFARQDFDDRVFTNPYEPVDLERVMTARKKYFTTSAEKQNA
                     NAAREKKQETAE"
     gene            complement(62542..>65114)
                     /gene="cas3"
                     /locus_tag="ACHKTW_000842"
     CDS             complement(62542..>65114)
                     /gene="cas3"
                     /locus_tag="ACHKTW_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CRISPR-associated helicase Cas3'"
                     /protein_id="HFO6461912.1"
                     /translation="DVAAMGYLMVKRNCFGLADYFRQLGISDKEQAAQFFAWLLCWHD
                     IGKFARSFQQLYLPPELKIQEGARKNYEKISHSTLGYWLWNHYLSECQELLPSSSLSP
                     RKLRRVIEMWMPVTTGHHGRPPDRMDELDNFLPEDKAAARDFLLEIKPLFPLIEIPAF
                     WDDDEGIELIKHLSWYISATVVLADWTGSSTRFFPRVAHPMDIKGYWQKTLIQAQNAL
                     TVFPLKAKVAPFNGINTLFPFIENPTPLQQKVLDLDISQQGPQLFILEDVTGAGKTEA
                     ALILAHRLIAAGKAQGLFFGLPTMATANAMYDRLVKTWLAFYSPESRPSLVLAHSART
                     LMDRFNESLWSGDLVGSEEPDEQTFSQGCAAWFADSNKKALLAEIGVGTLDQAMMAVM
                     PFKHNNLRLLGLSNKILLADEIHACDAYMSCILEGLIERQARGGNSVILLSATLSQQQ
                     RDKLVAAFARGTEGLQEAPFLEKDDYPWLTHVTKSDVNSHRVATRKDVERSVSVGWLH
                     SEQECIARIESAVSQGKCIAWIRNSVDDAIKVHRQLLARGVIPASSLSLFHSRFAFSD
                     RQRIETETLARFGKYCSLQRASQVIVCTQVIEQSVDIDLDEMISDLAPVDLLIQRAGR
                     LQRHIRDINGQLKRDGKDERSPPELLILAPVWDDSPGDEWFGSAMRNSAFVYPDHGRI
                     WLTQRVLREQGAIQMPHAARLLIESVYGEDVVMPEGFARSEQEQVGKYYCDRAMAKKF
                     VLNFRPGYAANINDYLPEKLSTRLAEESVSLWLATCIDGVVKPYATGAHAWEMSVVRV
                     RRSWWKKHRDEFSLLEGEAFRLWCIEQRQDPEMANVILVNDDESCGYSATEGLIGKIG
                     "
BASE COUNT        15411 a        17759 c        16807 g        15137 t
ORIGIN      
        1 attaaaatat ttatctttca ttttgagatc aaaataacac ttttaaatct ttcaatctga
       61 ttagattagg ttgccgtttg gtaataaaac aataaatcct gaaggagaga acaatgatag
      121 aaaccattac tcatggtgca gagtggttta tcgggctgtt ccaaaagggc ggagaggtgt
      181 ttaccgggat ggtgaccggc attcttccgc tgttgattag cctgctggtt atcatgaacg
      241 cactgattaa ttttatcggt cagcatcgta ttgaacgttt tgctcaacgt tgcgccggta
      301 accctgtttc ccgttaccta ctgttaccgt gcattggcac gtttgtcttt tgcaatccga
      361 tgaccttaag cctgggtcgc tttatgccgg aaaagtacaa acccagctac tacgcggcgg
      421 cctcttatag ctgccactca atgaatggcc tcttccccca tatcaaccct ggcgaactgt
      481 ttgtttatct tggcattgcc agcggtctga caacgctgaa cctgccactt ggcccactgg
      541 cggtgagtta tctgctggtt ggtctggtca ccaatttctt ccgcggctgg gtgaccgatc
      601 tgaccaccgc catttttgag aaaaagatgg gcattcaact tgaacaaaaa gttcacctgg
      661 caggagcaac atcatgacgc atattcggat cgaaaaagga acgggtggct ggggcggccc
      721 gcttgagctg aaagccacac cgggaaaaaa aatcgtctat atcaccgccg gtacccggcc
      781 tgcgattgtt gacaaactgg cacagcttac tggctggcag gctattgacg gatttaaaga
      841 aggtgaaccc gcggaggcgg aaattggtgt cgcggtaatc gactgtggcg gcacattacg
      901 ctgcggcatc tatccgaaac gacgtattcc caccattaat atccactcga cgggcaagtc
      961 cggtccgctg gcgcagtaca ttgtggaaga tatttatgtc tctggcgtaa aagaagaaaa
     1021 catcactgta gtgggtgatg cgacaccaca accctcttcc gtgggccgtg actatgacac
     1081 cagtaagaaa atcaccgaac aaagcgatgg tttactggcg aaggtgggaa tgggcatggg
     1141 gtccgccgtt gcggtgctgt ttcaatctgg tcgtgacacc atcgacactg tattaaaaac
     1201 cattctgccg tttatggcat tcgtctcggc gctcattggc atcattatgg cttctggcct
     1261 tggtgactgg attgcccacg gtcttgctcc gctggcgagc catccactgg gtctggtcat
     1321 gctggcgctc atctgctcct tcccactgct ttcacctttc ctcggcccag gcgcagttat
     1381 cgcacaggtt atcggcgtat tgattggcgt gcagattggt ctcggcaata ttccgccgca
     1441 tctggcttta cctgcactgt ttgccatcaa cgcgcaggcg gcctgcgact tcatcccggt
     1501 cggtttgtcg ctggcggaag cccgtcagga cacggttcgc gtcggtgtcc cttctgtact
     1561 ggtgagccgc tttttaaccg gcgcgccgac tgtactgatc gcctggtttg tctccggttt
     1621 tatctatcaa tagaggctga aacatgaccg ttatttatca gaccaccatc acccgtatcg
     1681 gcgcgagtgc cactgacgcc ctcagcgacc agatgctcat cacctttcgt gaaggcgcgc
     1741 cagcggacct cgaagagtat tgcttcattc attgccacgg cgagttgaaa ggtgcactcc
     1801 atcccggttt gcaattttca ctcgggcagc atcgctatcc ggtgaccgct gttggcagcg
     1861 tggcggaaga caaccttcgc gaactgggtc atgtcaccct gcgcttcgat ggtttaaacg
     1921 aagcggaatt tccgggcact gtccatgtgg caggccctgt acccgacgat atcgcgccgg
     1981 gatcggtttt gaagtttgaa tctgttaagg agtaaaaaat gaatcaggtt gccgttgtca
     2041 tcggtggtgg gcaaacctta ggcgcgttcc tgtgccacgg tctggctgcc gaggggtatc
     2101 gcgtcgcggt tgtcgatatt cagagcgaca aagccgcaaa tgtggcacaa gaaattaacg
     2161 ccgaatatgg tgaaggtatg gcgtacggtt ttggtgctga cgccaccagc gagcaaagcg
     2221 ttctggcgct ctctcgtggg gtagatgaaa tctttggtcg cgtggatttg ctggtctata
     2281 gcgccggaat agcaaaagca gcctttatca gcgacttcca gctcggcgat tttgaccgtt
     2341 cgctacaagt gaatctggtg ggttatttcc tgtgtgcccg tgaattttcg cgtttgatga
     2401 tccgcgacgg gattcagggt cgcattattc agatcaactc gaaatccggc aaagtgggta
     2461 gcaaacacaa ctctggctac agcgcagcga aatttggtgg cgtcgggctg actcaatcac
     2521 tggcgctgga tctggcggag tacggcatta cggtgcattc actgatgctc ggtaacctgc
     2581 tgaaatcgcc gatgttccag tcactgttgc cacaatacgc gaccaagctg ggtatcaaac
     2641 cggatcaagt cgagcagtat tacatcgaca aagtaccgct caaacgcggc tgcgattatc
     2701 aagatgtgct gaatatgctg ctgttctacg ccagtcctaa ggcgtcgtac tgcaccggac
     2761 agtcgatcaa tgtcaccggc ggtcaggtga tgttctgatc aacagcggag atccgttaag
     2821 gatctccgtg agactataga atgcctgatg cgctacgctc atcaggcata caggacttcc
     2881 gccactacat taaggaaaag ttatggtatc cgcactcatc accgtcgccg ttatcgcctg
     2941 gtgtgcgcaa ctggccttag gcggctggca aatttctcgt tttaaccgtg ccttcgacac
     3001 actatgccag caagggcggg ttggcgtggg ccgttccagc gggcgcttta aaccgcgggt
     3061 cgtggtcgcc atcgccctgg acgatcagca gcgcatcgtc gacaccttgt ttatgaaagg
     3121 actgaccgtc ttcgcccgac cgcaaaaaat tcccgcaatt accggtatgc atgcgggtga
     3181 tttacagccc gatgtgatct ttccccatga tccactatca cagaatgctc tatcattggc
     3241 gcttaaactg aaacgtggat aatttcgttg tgaatgttac ttgcttgcga agttatcatt
     3301 ttgaaaccta aatcaggtaa tcacgcccat gaaacctcgt cagcgtcagg ccgccattct
     3361 ggagtatctg caaaagcagg gtaaatgctc ggttgaagaa ttggcgcaat actttgacac
     3421 cacaggcaca accattcgca aagatctggt cattctggaa catgccggaa ccgtcattcg
     3481 tacttatggc ggagtggtgt tgaataaaga ggaatccgat ccgcctatcg atcataaaac
     3541 actcatcaac acccacaaga aagagctgat tgcagaagct gccgttagtt ttatccatga
     3601 tggcgattcg atcattcttg atgctggcag taccgttttg cagatggttc ccctgctctc
     3661 gcgctttaat aacatcacgg tgatgaccaa cagcctgcat atcgtcaatg cgctatccga
     3721 actggataac gaacaaacta tcctgatgcc aggcggaacg tttcgtaaaa aatcggcctc
     3781 atttcacgga cagctggcag agaatgcctt cgaacatttc agtttcgata aattgtttat
     3841 gggcaccgac ggcatcgatc tcaatgcggg cgtaaccacc ttcaacgagg tttataccgt
     3901 cagtaaggca atgtgcaatg ccgcgcgcga agtgattttg atggcggact catcaaagtt
     3961 tggccgtaaa agccccaacg tagtttgcag tcttgaaagc gtcgataagc tgattaccga
     4021 cgcaggtatc gatccggcgt ttcgtcaggc gctggaagag aaagggatcg atgtgatcat
     4081 aaccggagag agcaatgagt gaagcactac agaacgcggg acgtcagacg ttaatgctgg
     4141 agttgcagga agcaagccgt ttaccggaac gtctgggcga tgattttgtt cgcgccgcca
     4201 atatcatcct gcactgcgaa ggcaaagtgg tggtttcggg aattggcaaa tcgggccaca
     4261 ttggtaagaa aatcgccgca acacttgcca gtaccggcac accggctttt tttgtccatc
     4321 cggcagaagc actgcacggc gatctgggga tgattgaaag ccgcgatgtg atgctgttta
     4381 tctcttactc cggtggcgcg aaggaactgg atctgattat tccgcgtctg gaagataaat
     4441 ctatcgcgct gctggcgatg accggcaaac cgacgtcacc gctgggcctg gcggcaaaag
     4501 cggtgctgga tatctccgta gaacgcgaag cctgcccgat gcaccttgcg ccgacctcca
     4561 gcaccgtcaa taccctgatg atgggtgacg cgctggcgat ggcggtcatg caggcgcgcg
     4621 gatttaatga agaagatttt gcccgctccc acccagccgg ggcactgggc gctcgcttgc
     4681 tgaataaagt gcatcatctg atgcgccgtg acgatgccat cccacaggtg gcgttaaccg
     4741 ccagcgtgat ggatgcgatg ctggaactca gccgcaccgg tctggggctg gtggcggtat
     4801 gtgacgctca acaacaggta caaggcgtct ttaccgacgg cgatttacgt cgctggctgg
     4861 ttggcggcgg cgcactcacc acgccagtta atgaagcgat gacggtcggc ggcaccacgt
     4921 tgcaatcgca aagtcgcgcc atcgacgcca aagagatcct gatgaagcgc aaaatcactg
     4981 ccgcaccggt ggtagatgaa aacggcaaac tcaccggcgc aattaacctg caggatttct
     5041 atcaggccgg gattatttaa tccttcattc ccagacgttt cgccagccga tgcaggttgg
     5101 cgacgtcggt ttccagcatc cgcgcgcagg cagcccagtt gtgatgattt tgtgccagcg
     5161 cctgacgaat agtttcacgc tggaacgctt ctgtcgcttc acgcaggttt tgcttaacaa
     5221 cgggcaccgc cgccacttct ggcgtcggca acgtcacctc aggaaaagca aaatgttgcg
     5281 cctcaagaat cacttcatcg ccgttgcggg tggctctcga cagcactacc gcccgatgaa
     5341 tagcatgttc cagttcgcgc acgttccccg gaaaacgata gtgttgcagt aaatttcgcg
     5401 ctccggcact taataccacg cgggagagcc ccagccgcaa acgacactgc tcgcagaaat
     5461 accccgccag cagaatgaca tcatcgcccc gctcacgcag cggcggcacc gaaagtggaa
     5521 acacgctcag gcgatgaaac aagtcagcgc gaaatcgccc tgccagcacc tcttcgcgta
     5581 agtcgcggtt agtcgccgcc agcacgcgca catcgacccg caaactgcgg tcatcgccaa
     5641 cgcgctgaat atcgccatac tgcaacaccc tcagcagctt ggcctgcaat gccaacgaca
     5701 actcgccgat ctcatccaga aacagagtgc cgttatccgc catttcaaac ttcccgctgc
     5761 ggttactgat agcgccagta aacgctcctt tcacatgccc gaacagttca ctttccgcca
     5821 cactttccgg cagtgcggca cagttgagat agaccagcgg attcaccgcc cgtggcgagg
     5881 cttcatgaat cgctttcgcc accagctcct taccggttcc cgtctcaccg ctgatcagga
     5941 cgttgagatc ggacgccgcc acaatctcaa tctctttttt cagttgcgtc atgccagggg
     6001 acaagccaat catctgcgtc tgtttcaccg cttcaaacgg cgtggcatcg cctggcatca
     6061 tattctggct ttccagttgt tcaatcagca acgcattgct taacgctccc gccgccagcg
     6121 cagcaatcag ccgtagctct tcgtcgctga aaacatcgaa ctgatcgggc tgcatcccgt
     6181 cgagcgtcag tgcgccgatc aggttttgcc cggcaaacaa tggcagacca acgcaggcgt
     6241 gaaccttcag actctcctgc ccaggaatca aaccgtcata gggatcgggc aattcgctgt
     6301 ctgcgggaaa gcgcaccaca tccccggcgc gggcaatcgc ttccagccgt ggatgccctt
     6361 ccagcgcaaa gcgtctaccg agtacatcct ttgccagacc gtcgatggca agcggaataa
     6421 actgccgcga atcgtaacgt agcaacgcag acgcatcgca ctccagcacc tgacgtagcg
     6481 tggtgatcag gcgctgaaaa cgatcctgat gaccaatccc acgctgcaat tcgatggcga
     6541 tattcgccag cacatcaacg gaaaaactca tctttgcctc actgtcaatt tgactataga
     6601 tattgtcata tcgaccattt gattgatagt cattttgact actcattaat gagcatactt
     6661 ttatttatat agtaaaaaca atcagataaa aaactggcac gcaatctgca attagcaaga
     6721 catcttttta gaacacgctg aataaattga ggttgctatg tctattgtgg tgaaaaataa
     6781 cattcattgg gttggtcaac gtgactggga agtgcgtgat tttcacggca cggaatataa
     6841 aacgctgcgc ggcagcagtt acaacagcta cctcatccgc gaagaaaaaa acgtgctgat
     6901 cgacaccgtc gaccataaat tcagccgcga atttgtgcag aacctgcgta atgaaatcga
     6961 tctggcggat atcgattaca tcgtgattaa ccatgcagaa gaggaccacg ctggggcgct
     7021 gaccgaactg atggcgcaaa ttcccgatac gccgatctac tgtactgcca acgctatcga
     7081 ctcgataaat ggtcatcacc atcatccgga gtggaatttt aatgtggtga aaactggcga
     7141 cacgctggat atcggcaacg gcaaacagct catttttgtc gaaacaccaa tgctgcactg
     7201 gccggacagc atgatgactt acctgacagg cgacgcggtg ctgttcagta acgatgcttt
     7261 cggtcagcac tactgcgacg agcatctgtt caacgatgaa gtggatcaga cggagctttt
     7321 cgagcagtgc cagcgttact acgccaatat cctgacgccg ttcagccgcc tggtaacgcc
     7381 gaaaattacc gagatcctgg gctttaactt accagtcgat atgatagcga cctcccacgg
     7441 cgtggtctgg cgcgataacc cgacgcaaat tgtcgagctg tacctgaagt gggccgctga
     7501 ttatcaggaa gacagaatca ccattttcta cgacaccatg tcgaataaca cccgcatgat
     7561 ggctgacgct atcgcccagg ggattgcgga aaccgaccca cgcgtggcgg tgaaaatttt
     7621 caacgtcgcc cgaagcgata aaaacgaaat cctgaccaat gtcttccgct caaaaggcgt
     7681 gctggtcggc acttcgacga tgaataacgt gatgatgccg aaaatcgccg ggctggtgga
     7741 ggagatgact ggtttacgct tccgtaacaa acgcgccagt gctttcggct ctcacggctg
     7801 gagcggcggt gcggtggatc gtctttccac gcgcctgcag gatgcgggtt tcgaaatgtc
     7861 gcttagcctg aaagccaaat ggcgaccaga ccaggacgct ctggagttat gtcgtgaaca
     7921 cggtcgcgaa atcgcccgtc agtgggcgct cgcgccgctg ccgcagagca cggtgaatac
     7981 ggtggttaaa gaagaaacct ctgccaccac gacggctgac ctcggcccac ggatgcagtg
     8041 cagcgtctgc cagtggattt acgatccggc aaaaggcgag ccaatgcagg acgttgcgcc
     8101 aggaacgccg tggagtgaag tcccggataa cttcctctgc ccggaatgct ccctcggcaa
     8161 agacgtcttt gaagaactgg catcggaggc aaaatgagta acggcattgt gatcatcggt
     8221 tcgggcttcg ccgcccgcca actggtgaaa aatattcgca aacaggacgc cactattcca
     8281 ttaaccctga ttgccgccga cagcatggat gagtacaaca aacctgacct cagccatgtt
     8341 atcagtcagg ggcaacgtgc cgatgacctt acccgccaga cggcgggtga atttgccgag
     8401 cagtttaatc tgcacctgtt tccacaaacc tgggtgacgg atatcgatgc cgaagcccgt
     8461 gtggtgaaaa gccagaataa tcagtggcaa tacgacaagc tagtactggc aaccggtgcc
     8521 agtgcctttg tcccgcctgt gcctgggcgt gagttaatgc tgacgttaaa tagtcagcaa
     8581 gagtatcgcg cctgtgaaac gcaactgcgg gatgcccgac gcgtgttgat tgttggcggt
     8641 ggtttgattg gtagcgaact ggcgatggat ttttgtcgtg caggcaaagc ggtcacgcta
     8701 atcgacaacg ctgccagtat tctggcgtcg ttaatgccac cggaagtaag cagccgcttg
     8761 cagcatcggt tgacggagat gggcgttcat ctgttgttga aatctcagtt gcaggggctg
     8821 gaaaaaacag attctggcat tctggcaacg ctggaccgcc agcgcagtat cgaagtggat
     8881 gcggtaattg ccgccaccgg actgcgcccg gaaaccgccc tggcacgacg cgccgggctg
     8941 acgattaatc gcggcgtttg cgtcgatagt tatctgcaaa ccagtaatgc cgatatttac
     9001 gcgctgggcg attgcgcgga aattaacggt caggtattgc cgttcctcca gccgattcaa
     9061 cttagcgcga tggtgctggc aaaaaatctt ctcggcaata acacgccact gaaactaccg
     9121 gcgatgctgg tgaaaatcaa aacgccggaa ttaccgctgc atctggcagg cgaaacccag
     9181 cgtcaggatt tacgctggca aattaatacc gaacgccagg gaatggtggc tcgcggtgtt
     9241 gacgatgctg accagcttcg cgcctttgtg gtcagtgagg atcggatgaa agaggcattt
     9301 ggattgttga aaacattgcc gatgtaggtg ggctactgtg cctaaaatgt cggatgcgac
     9361 gctggcgcgt cttatccgac ctacggggac gcatgtgtag gccggataag gcgtttacgc
     9421 cgcatccggc aatggtgtcc aaatgcaaca cgttttatcc gttctggact tcacccgcta
     9481 accaacgcgc cgcagcaata accccctgcc ccagagacaa accgccatca cccgccggta
     9541 aactctgtgg aaagagcaat gtgaaatcag cgagataatg cgccagacgt gcacgcagca
     9601 aacggttatg aataaccccg ccgctaaata ccagcgtagt gataccacgc atcgtggcct
     9661 gctcacgcat caacgcggca aaaccctgcg ccagcgcatc atgaaacgcc cacgcgcgtt
     9721 gattaaccgg tgcctgccag ttcagccact gctgccagaa agtggcgaga tccagttgat
     9781 tgtccaccag cggcattgtc accggatgcg tcactccgtg gcacgaagct gcgagtgcct
     9841 ccagagcaca agccgcttca ccttcataac ttaacgtggc tggcgcacag cccagtgccg
     9901 ccgccacggc atcgaacaac cgcccacacg atgacgccag cggcgcgtta attccacgct
     9961 caatggcccg cgccagcacg ctccagtttt gctgttgcac acttgctgtt tcagaataat
    10021 tctgccactc cggcacaaag cgcaggcact gcgccagcag gtttcgccac ggctgcgtcg
    10081 ccgccaaatc gccacccgga agcgccactg caggcaagcc gcccaggtgc tcacattcgc
    10141 gatagttcac ccgcagacac tcgccgcccc acaaagcgcc gttctccccc ataccgatac
    10201 catcgagcgt caaagcaatg acatcaccgc catccagcgg ccactgatgc tctgccagac
    10261 acgccgctgc gtgggcatga tgatgcagca ccgtttgcgt cggcatattc atttcgctcg
    10321 cccactggct ggagacatag cccggatgca cgtcatgcac aacgtattgc ggagtgaaat
    10381 cgtagatgtt ttgcatcagg cgtaacgctt cgcgccactg catctggatg ccatcgtcac
    10441 ttaaatcgcc cagatgctga ctcaacaccg cttgttcacc gcgcaccagg cagaaggtgt
    10501 ttttcagatc cgcgccaaga cacagcacag gcggaacatt tttaaagccc ggaggcaaaa
    10561 ccagcgcatc cggcacatac ccgcgtgaac ggcgcagcat ttcgccgctt tcgcgcacca
    10621 ccgaatcatc catccgctgc acgatgtcgc ggttatgtat caagaatccg tcggcaatgc
    10681 cctgcaaatc cgccagcgcc tgttcgttgc tgatagctgg tggtttaccg ctcaggttgc
    10741 cggaggtcat caccagcggg cattgcagtt cctgtaacag caaatgctgg agcgggttcg
    10801 caggcaacat taccccgact tcgttaaggt caggggcgat atcatcacaa agctcaggaa
    10861 cgtatttttt atccaccagc acaatcggcg cggcgggcgt ggtaagcaac tggcgcgcag
    10921 cgtctggtaa accgtcagcc actggcaaca tgaccgccag cggtttcgcc gggcgatgtt
    10981 tgcgcgcccg cagtgtcgcc accgcgttac tgttacgtgc atcgcaggca agatgaaatc
    11041 cgccaatccc tttgatggcg acaatgttgc ccatttttaa ctgtgcgata gccgcctgta
    11101 atgccgcctc ttgttctgca tgttcaccat gacttaccca ttcaagatgt gggccacact
    11161 ccgggcaggc caccggctgg gcgtggaagc gacgatcgag cgggtcgcgg tactctttgt
    11221 cacaagccgg acacaacgga aacgccgcca tcacggtaaa cgggcggtcg taaggcatgg
    11281 cgcgaataat ggtgaaacgc gggccgcagt gggtacagtt gataaacgga taacgataac
    11341 gccgttcgcc tggggtattc atttcggcaa gacaggcagg gcaagtagcg gcatcgggaa
    11401 caatttgcgt attcatggtg ccgcctgtgc tctggcgtat agtgaactcg gtgggcagtt
    11461 gtgaccagat gtacggctca cgctcgacgc tatcaatacg cgccagcggc gggcagtgct
    11521 gatgcaattg aacaagaaac gtttccgggt cttccagcag ccggacttct acgccatcgc
    11581 cgtcattaca gacatcgccg tgaagattta attgctgtgc cagctgccag acaaacggac
    11641 gaaaaccgac gccctgcact ttgcctcgaa tacgcagttg gacaccgcaa gatgtgtttt
    11701 ttgccattga gttattcccg ccatcatgaa ttgcgtaacc cgccctgccg gacacgactg
    11761 cgtcgtatcc ggcagtctca ggtcggcgat accgccagct ccgtattcta cgaatatttc
    11821 cgggaattcc tttgatgcca gaacagttct ataagatttt tagaacatca gcgccgtacg
    11881 gcgacgtttt tctgcgctca gttgttcaag tttattacga tcgacacaaa tcagcgcatg
    11941 ggtcgggcaa gccgccatac acgccgggcc gtcttcgcga tggttgcaca ggtcgcattt
    12001 attggcttca gctttgtcag cccgtacatt cagccccgcg ccgctgttgc ggatcaccgg
    12061 acgtaccacc acttccatcg cgccatacgg gcaagccacc acgcaggttt tgcaaccaat
    12121 gcaacgttcc tgcatcacat gaacaaaccc tttatcacgg ctgatagcac cattcgggca
    12181 gacgttagcg cacggtgcat cttcacactg acggcaaact gtcgccgtgg aaatgttcac
    12241 acctttaatg acatggatac gcggtaaaaa tgtttccggg gtcagcgatg cacagtcctg
    12301 attttcctga tgagaaacca cgcacgccac ttcacaggta cggcaaccaa tacatttact
    12361 cgcgtcagca atgatgaaac ggttcatcaa attctccagc aatgacagtt aatgcgccga
    12421 tacattcaca aatcatgcca gtttttaatt cactgttatt taaggaaatt aatttctgta
    12481 atgcaggaaa aaagatgtca tcgacactag tgacgatgac atgtgatgac aatgtttatc
    12541 gcgaaagagc aatgagtgag tcgcggcgga tcagttttcc gctgaaggtt ttcggcggtg
    12601 agaaatcccc gccatcgagc ataaaaatca gccgtccaat aatttcctga atcatctctg
    12661 ttaccggaat ttttacgctg gagagcgccg gaacggtgta gggggcaatt gcgatatcat
    12721 cgaatccgat aaccgacacc tgctctggca ccgctacgcc gcgctcgtgc aacgctttga
    12781 tcgcacctat cgccatatcg tcgttactgg caactaacgc gctaaattta gccccacgtt
    12841 cgagcaacgt ttctacccct tcggccccgc tggcgggcgt ccatttaccg ttagcgataa
    12901 gtttttcatt gagcgcaata ccatgctgcg acagcgcgtc tttatacccg gcaagacgtt
    12961 caacgctggt gggggaatcc atcgagccgg taaggaaagc aatctcctga tgtccggcgt
    13021 ttatcaactc tgccacggca ttaaagctgg tctgtttatg atcgcaccag acgctatggc
    13081 tgctgttttt gcgcaggcgg cgattaagca ccattatcgg ctgactgtgc gcgtcaatga
    13141 tgtcatcgat ctcatccacg cttaaaaaac gcgggtaaat cataatggcg tcgcagcgca
    13201 aatccagcag atactgaatc gcctggcgct cttcttcggc gctgtgttta ccatctgcca
    13261 atagcaactg ccgccctttc tcttccgcca ttcgcgcggc atgaaagagt aattcactaa
    13321 aataaatgcc gtggtaaagc gtgttggtca ctaccagccc cagcgtctga gtactcttcg
    13381 ccgacagatt gcgcgccagc aagtttgggc ggtaaccgct ctcttctacc gcctgaaaca
    13441 cgcgatcttt agtctcctgg ctgacgtagc cattacctga aagcacgcgg gaaacggtcg
    13501 cttttgaaac cccggcgcgc ttcgccactt ccagcatcgt cgtcatcatt ttcatccctt
    13561 tacacgcaat caacgcagtg tactgcaccg tttgccgatt gtccttgcgc aatcggcggg
    13621 aaaaatattc aggtgaccgg tttcgcaaat ataaaaaatg aacaattaac tctcttgctt
    13681 atttagtgac aactattcat gattttgtga aaccggtttc ctaattccgt ttcagcatcg
    13741 gcatttttcc gttacgtcga ctgataacaa ctacatctac cctactgata acaggataaa
    13801 atccgatggc caaaaattat gcggcgctgg cacgctcggt gatagcggca ctgggcggcg
    13861 ttgataacat ctcggcagtc acgcactgta tgacgcgttt gcgctttgtt atcaaagatg
    13921 accagcttat cgacagcccg acgttaaaaa ccatccccgg cgtgctcggc gtggtacgta
    13981 gtgacaacca gtgtcaggtg attatcggca ataccgtttc acaagccttt caggaagtcg
    14041 tcagcctgct gccgggagat atgcagcccg cacagcccgt gggtaaaccc aaactcacgc
    14101 tacgtcgcat tggtgcgggg atcctcgatg cgctgatcgg caccatgtca ccgctgatcc
    14161 cggcgattat cggcggatcg atggtcaaac tgctggcgat gatcctcgag atgagcggcg
    14221 tgctgacaaa aggatcgccg accttaacca ttctgaatgt gattggtgac ggtgctttct
    14281 tcttcctgcc gctgatggtc gcggcttcgg ccgccatcaa atttaaaacc aatatgtcgc
    14341 tggcgattgc catcgcgggt gtgctggtac atccgagttt tattgaactg atggcgaaag
    14401 cggcccaggg tgaacatgtc gaatttgccc tgataccggt caccgcggtg aaatacacct
    14461 acacggtgat cccggcgctg gtgatgacct ggtgcctgtc atatatcgaa cgctgggtgg
    14521 atcgcattac accggcggtg accaaaaact tcctcaagcc aatgctgatt gtgttgattg
    14581 ccgcaccgct ggcaatcctg ctgattggcc cgattggtat ctggatcggt agcgccattt
    14641 cggcgctggt ttacaccatt catggttatc tgggctggct ttcagtcgcc attatgggcg
    14701 cgctgtggcc tctgctggta atgaccggga tgcaccgcgt ctttacgcca accatcattc
    14761 agaccattgc cgaaaccggc aaagaaggga tggtcatgcc gtcagagatc ggcgctaacc
    14821 tgtcgctggg cggttcatca ctggcggtgg cgtggaaaac gaaaaacccg gaactgcgcc
    14881 agacggcgct ggctgcggcg gcatcagcca ttatggcggg gatttccgaa ccggcgttat
    14941 acggcgtggc gatccgcctg aaacgtccgc ttatcgccag tcttatcagc ggttttattt
    15001 gcggcgcggt tgccggtatg gcggggcttg ccagccactc aatggcagcg ccggggttat
    15061 ttaccagcgt gcaattcttc gatccggcga atccaatgag catcgtctgg gtgttcgcgg
    15121 tcatggcgct ggcggtggtg ctgtcgttta tcctcacact attgctcggc tttgaggata
    15181 ttcctgttga ggaagcggct gccgaggcgc gaaagcatca gagcgcacaa ccgaccgtcg
    15241 ccaaagaagt aagtcttaat tgaggatgaa aatgtcagta tttccagaag gttttttatg
    15301 gggcggcgcg cttgccgcca accagtctga aggtgcgttc cgtgaaggtg gcaaaggact
    15361 gaccacggtc gatatgatcc cgcacggcga gcatcgaatg gcggtgaaac tggggctgga
    15421 aaaacgtttt cagttgcgcg atgacgagtt ttatcccagc catgaggcga cggattttta
    15481 tcatcgttat aaagaagata tcgccctgat ggcagagatg ggattcaagg ttttccgtac
    15541 ctcaattgcc tggagccgcc tctttccgca gggcgatgaa atcacgccca atcagcaggg
    15601 cattgctttt taccgttctg tctttgaaga gtgtaaaaag tacggtatcg aaccgctggt
    15661 cacgttgtgc cacttcgatg tgccgatgca tctggtcacc gaatatggct cctggcgtaa
    15721 ccgcaagctg gtggagtttt tcagccgcta cgccagaacc tgctttgaag catttgatgg
    15781 tctggtgaaa tactggctaa ccttcaatga aatcaacatt atgttgcata gcccgttctc
    15841 cggcgcgggt ctggtgtttg aagaaggtga aaatcaggat caggtgaaat atcaggccgc
    15901 gcatcaccag ctggttgcca gtgcgctagc caccaaaatc gcccatgagg ttaacccgca
    15961 aaatcaggtg ggctgtatgc tggcgggcgg taacttctac ccttacagct gcaaaccgga
    16021 agatgtctgg gcggcgctgg agaaagatcg ggaaaacctg ttttttatcg atgtgcaggc
    16081 gcgcggcgcg tatccggctt actctgcccg cgtatttcgc gaaaaagggg taaccatcaa
    16141 caaagcaccg ggcgatgatg aaatcctgaa aaacaccgtc gattttgtct ctttcagcta
    16201 ttacgcctcg cgctgcgcct cggcggagat gaacgccaac aacagcagtg cggcgaacgt
    16261 ggtgaaatcg ctgcgtaatc cgtatctaca ggtgagcgac tggggctggg gaattgatcc
    16321 actcggtctg cgtatcacca tgaatatgat gtacgatcgt tatcagaagc cgctgtttct
    16381 ggtggaaaac ggcctcggcg caaaagatga acttgctgcc aatggcgaga ttaacgatga
    16441 ctatcgcatc agctatttac gcgaacatat ccgcgcaatg ggcgaagcga ttgcagatgg
    16501 cattccgctg atgggctaca ccacctgggg ctgtattgat ttagtttccg cctctacggg
    16561 tgaaatgagc aaacgctacg gttttgtcta cgtagaccgt gacgacgcag gcaacggcac
    16621 gctgacgcgc acgcgtaaga aatcgttctg gtggtataaa aaagtaattg ccagtaatgg
    16681 ggaagattta gagtaagtaa ccgtgccgga tgcggcgtga acgccttatc cggcctacat
    16741 agttcgtgcg acatgtaggc ccgataagcg cagcgcatcg ggcaatctgg ggtttggcat
    16801 cagtcaaaat caaagaaacc attccggctt aacgccaaat cgggctgaaa gtgccttaat
    16861 gtgggatatt gtcagggatc gttgtccaga caaaatctga ctgaccaggg atttagagcc
    16921 gatttcgttc ttcaaatccg cataggtcag attatgttga tcaatcaacg tgcgaagtaa
    16981 cgccacgcca accggcattg ccgcaacggc tttgtcgaac tctgcaaact tttcgttgtt
    17041 gttttcatac tctgcaatgc ggctcgccag aaagtcgata agtggattgg tgtcatcttc
    17101 ttcaatcaaa tattcgacca gttccagtgc ttcacgataa tcatcttctg agtcactgcc
    17161 tcccagaaag gggactgcat tgaccagttc tcttgttgct ttaacagcgc gtgcagcatt
    17221 agcagtcatt ctttgttctc tctgtagtaa cgtgacgtct gtctgcacgt aatccccctc
    17281 atagccactc tgcaaaaagt cagttctggt tcaccccata aagcacatcc ccctttatca
    17341 agcaacgctg cgtctgcgcc agatcttccg tttgcgggtc gcgcccggcg atgggtttga
    17401 ggacgagttt acggttgggg gtataagtaa aacgcgtgag cgcgggatag cttaacttgc
    17461 caaaccagcc gtgaacacgc tgccatttgg cgtcttttac ctgatacaga ctggttatcc
    17521 agtaaccgga atcaaactgc atatccagca attgcgtgtg gccattcctt tgtaaatcca
    17581 gcaaatcatc aatgccagtt tcacttgcgg tataaaaacc atcggactga aaaacacacg
    17641 ggcgaccttg ctgattaaag gtcatcagaa tcaaaaacgc gttaggtgcc agaccatttc
    17701 ctgaaatacc ccgccagata accagatcct gaatgccgtt acgatcaaga tcggcacggt
    17761 aaagttgcgc attacccgcc atattttgca gcacccccac aggcacagac caggatttac
    17821 cggcgcgatc tttaccgttc aattgccatt cactatcatc atttggcatc ttttcaagcg
    17881 tcacgccagc aacgccgact tttctaaaat gccagttttg ctcggcatca taaggcatgg
    17941 tcaggagagg tttcagcgcc cgcttttgcg cgtattcgct gtcactcatc ggcagatatt
    18001 cggtaaagct ggtggcctgt gcaggcagca ctaagctcaa taaaaaagcg cacatccctg
    18061 ttctgatgtt caattccctg aattccttaa tgaaaaacta ctctacttcc accgctaact
    18121 gcgcaaatcc accatttcct tcccagcctt ccagtcgttg ataaacggtt tcaaccgcat
    18181 ctttaatcgg ctgggtcatc gggtagtaaa agccgacgat atccggctgg atgccgagga
    18241 aaatcacttc gccaatatct tctttcaact ggtcgataag gtagttcaac ggcatgttat
    18301 gggtagtcat cataaacatc tcggcgatat catccgggtc gatgatgcgg atctcgccgg
    18361 ggtttagccc catatcagtg gcgtcgacaa tcagcagtcg cgtagggcgc agttcacgga
    18421 tggcgacgat gtcgttttcc ggtgcactac cgccgtcaat caccacccag ttacctttcg
    18481 gcgcggcggc gcacttttcc gccagcagcg ggcctgcgcc atcatcgccc atcatgctat
    18541 tgccaacaca gagtaaaacg tcagtcacgc agtctcctca ccatcaaata gatggcgttt
    18601 tcctgatgaa tatcatgcag catacttagc agcattgtac tccattcctg ctgctgcgaa
    18661 gtttgcgctg cccgggcttt atcgaatgcg ttggccagca tcggcacatg gttaatgtcg
    18721 atgacgatct caccatattt cggcacgcct tccattttgc ggcgggcttc gctgcccgcc
    18781 tccagagtgg caatccatgc cagatattca tcccacgggc aggtgagcgc cgcttccagg
    18841 caatcgataa ccccaaggtg gtgaccaatc gccaggctgt aatagaccac ctgctgcgcc
    18901 tcggcgggcg tggcatcgtt ctcatcaata aatttacggc tcagttgact gaacaccacc
    18961 ttttcactca tcggatacgc gcctcttcta caacatgatt cagatggctg acaatctcgt
    19021 tcagacgcgg atcgttttcc gcttccagcc agcgtgcaac ctgttcttcg ccctgcccta
    19081 actgtgtcag gaagtcatcg gcaatctgac gaccgtaacg ataacctgcc aggcggcgtg
    19141 cttcgcgatc cactttcaca cgcagcggct gcaccatatc gccatgcagg atttccgccg
    19201 gttgttcatc ctgctcaccc gggccacggg cgtgaatttt ctgctccagc aggccgagcg
    19261 ccattgcaaa gccgtacagc gtggcggcag gcgttggcgg gcaaccaggg atgtagacat
    19321 ctaccgggac gattttatcc gtaccgcccc acacgcagta gagatcgtgg aagatcccgc
    19381 cactgttacc gcaggcaccg taggagatac agattttcgg gtccggcgcg gactgccacg
    19441 cacgcagcgc aggggatcgc attgcacggg tgaccgcgcc ggtaaacagt aaaatatccg
    19501 catgacgcgg tgaaggaacg actttaatgc cgaagcgttc tgcatcaaac agcggcgaaa
    19561 gcgtggcgaa aatttcgatt tcgcagccgt tgcagccgcc gcagtccacg cggtaaacgt
    19621 aggcagaacg tttgattttt ttcagtaacg acgccttcat gctggcgatg gattcatcca
    19681 ccgtcatcgg gaccggaatg ccgttggcgt cacgggggcc taataaattg ctcatcagat
    19741 ggcctctttc atatggcgag tcagttcaat acggtcggac ggcaccaggc atttctggcg
    19801 cttacattcc gggcaagtct caaagctttc gcggtggttt tccgcgcggc tgtcaccatt
    19861 gtgcttaagc agcgcaatgg cgtagtcgat ctctttctgg acggcgaaag gacgattgca
    19921 gacgcgacag ctgcacagca caaaacggga ctgttgcagg aaatcttctt tcttccacac
    19981 cgccagttcg tactcttgcg acagtttgat cgctgccgtc gggcagactt cttcgcagcg
    20041 tccacagaag atgcagcgcc cgagattaaa ctcccacgca agctctcctg tggcgaggtc
    20101 agtttcaacc gttaaggcgt ttgacgggca ggcattgacg caggccgcgc agccgatgca
    20161 ctgctgcggg ttctgctctg gcttaccacg gaagttttta tcaaccgcaa tcggctccag
    20221 cggataagac gaggtcgccg tgccggtttt gatgactttt ttgataaagg taaacatggc
    20281 gattccttat ttcagcggcg agtttttacg ctcaatgctg taacgctcga gttctttgta
    20341 cggcaccact ttgctctttt tcttacgcac atcgaccacg gtcatgcggt cggtacagga
    20401 gtagcaaggg tccaggctac cgataatcag cggcgcatcg gaaacggtgt taccgcgcag
    20461 catgtagcgc agggtcggcc agttcgcgta ggtcgcggca cggcagcgcc agcggtacag
    20521 cttctggttg tcgccggtca tgctccagtg gatatcgtcg ccgcgcggcg cttcggcaaa
    20581 gcccagcgcg aaacggtgcg gaatgtaggt aaagccttcc accatcagcg gaccgcccgg
    20641 caggttatcc agaccgtagt cgatcatgtt cagcgcggta taaacttcgt tgatacgcac
    20701 tttcagacgg gaaataacgt cgcagccctg ctcgctgtgg acttccattg gcagcaggcc
    20761 ataaccgaca aacgggtgat cggcgcgagt atcgcgggcg tgaccgctgg cgcgaaccat
    20821 cgggccaacg ttactgaaat cgcgggcgat ttccgggtcc agacgaccaa tgccgacggt
    20881 gcgctgttcc atgttcggcg tactcagcag cacatccacc agctcctgca cttcacgacg
    20941 catctgttgc gccagctggc gggtctggat catgtcatct ttcaacagat cgcgacgaat
    21001 cccgccgatc aggttcaggc cgtaggtttt acgcgcaccg gtgaggatct ctgccatttt
    21061 catggaggtt tcacgcacgc ggaagaactg cataaagccg gagtcaaagc cggtaaagtg
    21121 gcaggcgaga ccgaggttga gcagatgcga gtgcaggcgt tccacctcca gcagaatggc
    21181 gcggatcatc tgcgcacgtt ctggcacctg aatacccatc gcgttttcta ccgacgtggt
    21241 gtaggcggtg ctgtgggcaa agccgcagat cccgcacaca cggtcagaga ggaaggtcac
    21301 ttcgttgtaa cccatacggg tttccgccag tttttccatg ccgcgatgaa cgtagaacag
    21361 acggtagtcg gcgtcgataa tgttttcgcc atcgacgaac agacggaagt ggcccggttc
    21421 gtcagaagtg acgtgcagcg gaccaatcgg cacgacgttg tttttcttgt cgcccagttc
    21481 gttgatgaac tcgtaggttt cagcatcggt ggtcggcgcc ggacgctgac gataatccat
    21541 gctgtcttta cgcagcggat aaagttcatc cggccagtca tccggcagca ccagacggcg
    21601 ttcatccggc agaccaaccg gaatcaaacc gtacatatcg cgcacttcgc gctcgcccca
    21661 cacagccgcc ggaacgcgcg gcgtcacgga cggatactcc ggtttgttgg cgtcaacttc
    21721 gacgcgaacc gttacccaac acttagtgcc cttctccatc gacagcacgt aataaacggc
    21781 gtaatgacca ttcagtttgc gttcgtcgtt accaaacagc accgacagcc agccaccctg
    21841 tttgtagtaa agaaactcca ccacttccgg caggtagttc acctttacgg tgacagtcag
    21901 ctgatctttg gtctgccagg cgtggtccag cacgacgccc ggaaatgcct cattcagcgc
    21961 ggcgagataa tgttgaccta atttttcttc agacatgctc aaactctctt taatcacgcc
    22021 gccagcaagg agacgaacgc taaaaatgca aagccaaacc cagcccaggt aatgcgcgga
    22081 gtaatatcaa gacgcagacg ggccatgctg ttttcgaaca gcgcgataac caggacgccg
    22141 accaccagtt tgacgatggc aatcaccagc gccagcagca gtccaccggc ggtgaaggtt
    22201 tccatttgtc cccacggaat aaacaccccg acgaacatct gcaacaccac cagctgtttc
    22261 aggctgatac cccatttcat gacgccaaag ccgctaccgc tgtattcgga gagcggacct
    22321 tcctgcaact cctgctcggc ttccgccagg tcgaacggca gtttgcccat ttcgataaaa
    22381 gtggcgaacg cacaggcaca aagcgccagc accagcggga tgctctggct cagcggccag
    22441 tgataaacgg tgtcggtgat gttgctgatg ttggtggaac cggcaacctg tgcggcaacc
    22501 cacagaccaa gcagcagcat cggttcgacc agcacgccaa gcatcgcttc acggctcgcg
    22561 ccgatagcag taaacgggct accggtatcc agaccggaaa tggcaaagaa gaaacgcgcg
    22621 atggcaaaga gatacagtaa ggtgatcaaa tcgcccagtt gcggcagcgg agaaccgacg
    22681 gtcaccaccg gcagcgcagt agcgatagtc agcatgacgc ccaccatcac atacggcgtc
    22741 aggcggaaca cccagccgga ggcatccggg ccaacgctct ggcggcccag cagtttgata
    22801 atgtcacgat actcctgcaa cacacccggc ccacgacggt tatgtaagcg ggctcgcgcc
    22861 acgcgggtaa taccggagag cagcggcgca acggcaaata gcaccagcgc ctgaattaac
    22921 ggatataaaa cactcattct caggctcctc gtgaaacaat aatcaccacc agcaccgcca
    22981 gttcaaccag cgccatccgg cggaacagca acgcactccc ctcgcactgc cagcccggca
    23041 ccagagacac cggattcagc catttgcgta gtttcagcac cggcgcaaac gcctgtttca
    23101 ccggcatggc aaaaccgtga gcggtaatca ccattgattt ttcgtggtcg tagccgcaca
    23161 cccaggccga gccgcgggaa cgcgacggca aacgatcgcc tttgcaaatc gccataatga
    23221 tgaatggcag cagcgggcag gcaatcagca gcaatgtgat catgggttga gaaacggtgg
    23281 tgttagcagg ctccagaggc agaggtacag cagcagagag catcggcagc agccacggcg
    23341 cagcaacacc gccaattacg cagcaaatcg ccagtgccac tacgcttacg ctcatcagga
    23401 gcggcgcaca ggtggcgttt tcggcttctt tggtacgcgg cgcgccgagg aacgtgacgc
    23461 catagacttt cgccatacac atcaccgcca gcgcaccggt aattgccaga cccacagcga
    23521 gcagcggccc cagcagacgg gcaacaaacg cgccactatt gctcagtttg aagaaggatt
    23581 gatagataac ccattccccg gcaaacccgt tcagcggcgg cagcgcagcc attgccatta
    23641 acccgactaa catggcgatg gagataaccg gcattttctt gccaatacca ccgagtttct
    23701 cgatatcgcg atgaccggta cggaaccaga cgctccccgc cccgaggaac agtacgcttt
    23761 tgaacaggct atggttaagc agatggtaca gaccaccgac caggccaaga gcaatcagcg
    23821 ccggttgttc gagcgcgata cccgttacgc cagcgcccag ccccagcagg ataatgccga
    23881 tattttccag ggtgtggtaa gccagcaggc gctggatgtt gtgttccatc aacgcataca
    23941 gaccaccgac gaacgcggtg atcatgccga gcaccagcag cgcgatcccc caccacagcg
    24001 gcgcgttacc acccagcagc gacagggtta aaatgcccag caggccaatt ttcatgacta
    24061 ccgtagaaaa caacgcggca gctggcgcag aggcgttagc atgtgcctgc ggcacccagc
    24121 cgtgcagcgg aataatcccg gccagcaggc caaagccaat cacaccgagc agccagatat
    24181 cggaaccgag cggcagctgt tgcatacgca tatccagcag gcgcagatcc agcgtgccat
    24241 aacgctgcca cagcagccag caagcaatcg ccagcagcag agtgccaaga cgccccagcg
    24301 caaaccacag tttgccctct ttgctgttgc tggtaaggaa caccgcacac aaggccatga
    24361 cttcagccat taccacgaac atgccgaggt tgctggcgat gacggcgcag acggcggcag
    24421 ccatcaacat attgatctgc aagccgttgc acttcacctg cgcgtggcga tgccagtcaa
    24481 tgttgtagag gctgacaaac agaccgcaca gaccgagcgt aatcagccaa atcgcgttaa
    24541 gcggagagat ttgcacatcg tagcttacca gcgacagcgc accgctcacg ccaaccgcgc
    24601 cagtcagtac agtgaagccc gcggctgccg tatacagact accaaccgcg ccgccaattc
    24661 cggctatcca gccacttagc gtcttttgaa aagaaaagag aaatgccaga acagcggcgg
    24721 cgacaaacca tgccacgccg ctattgatca gggaaattgc gctcatttag cctctccact
    24781 ttgagcctgc tgaaacaagg tgagatcgcc aaagtccgtg ttaaaggtca gctcacgctt
    24841 acgtttgctg acgcgggcga tatcggtgtt atccaccaga tgcagggctt tagtcgggca
    24901 catccgcacg caggccggac cttgttcatc aaaactgcaa aggtcacatt tgacggcgat
    24961 cgcgcgaata cccggcaccc agtcaagcag cgtactgata cgcgccggag caggcggtgc
    25021 cggtggcgct ttcggggtat tggcatttgc aggaatatcc agcggacggc tgccggaaaa
    25081 ttcaattgcg ccaaacgggc aggcgatgcc gcacagcttg cagcttacgc acaggctttc
    25141 attcaactgc acggccccat caacgcgggt gatggcgtta accgggcaga ccaccgcgca
    25201 gggtgcatct tcacagtggt gacagagctg cggcgcagat tctttttcat ttagcatcac
    25261 tctcaggcgc ggcattgatt gcaggccgtg ctggcgatgc gtctctgaac aggcggcctc
    25321 acaagtgtgg cagccgatac agagcgtgga gtcagcaatt acaaaacgat tcaccaggca
    25381 ttcctcaggt gattgtcatt tttgacgaaa acatgccgat gaaatgtcat tttcgacact
    25441 catcgacacg cccatcccca aacaggcgta acgcctgcaa aacgggcaaa gcctcagctc
    25501 atgctgccgg gctttgtccc tttaccagtt ggcttaaatt caccggcaca ttgttttcaa
    25561 tggcggtgta taagctgtcg taaacgccag cgattttttc gagatagtgt tccagaacct
    25621 gttcgcgatc gcggatgttg accttgccat caatcatgcc gtcaatgctc agttgcgcct
    25681 gcgcaatccg ctgtttgtct tcgccatctt ttgtttcgac gtaatactcg atggtgtggc
    25741 tgttaaagcc atccgccagg gtgacgtaaa tacgaaaatg ttcaaaaagg acgaaacaga
    25801 gttctttgtc cggcgcgcag ctcatggcgt gatgcaggcg cgctttcgat aacgtgatcc
    25861 cctgaaccag cgaattgtag tagatgtgcc actggtcctg taggcgacga tgccgctgtg
    25921 cgatgtaatc ggctttctcg cttatttccc aaatagtcat tgtcaggtta cccgtttaac
    25981 agagatgcca gctttaagca ttttttgtgc caacttttaa tttattgtta ttaaagagat
    26041 ttttaagcta aagatgaatt tcgtcgccgt gtcgacgtgt catttagaca tcatcgacat
    26101 tattcaccgc agggataatc aacactggca caattattgc ttgtagctga caatagttaa
    26161 tgggaggcga tatgcacgaa ataaccctct gccaacgggc actggaattg atcgaacagc
    26221 aggccgcaaa acacggcgca aaacgcgtaa ctggggtctg gctcaaaatt ggcgcatttt
    26281 cttgtgtcga aaccagctct cttgcctttt gttttgatct ggtttgccgc ggcagcgtgg
    26341 cggaaggttg taaactgcac ctcgaagaac aagaggccga atgctggtgt gaaacatgcc
    26401 aacagtatgt gacgctattg acccagcgcg tccgccgctg tccacagtgt catggtgaca
    26461 tgctacagat tgtggcagac gacggtttac agattcggcg gatagaaata gaccaggagt
    26521 gagcgatgtg tacaacatgc ggttgcggtg aaggcaacct gtatatcgag ggtgatgaac
    26581 ataaccctca ttccgcgttt cgtagcgcgc catttgcccc ggcggcacgc ccgaagatga
    26641 aaatcaccgg cattaaagcg cctgaattta cccccagcca gactgaagaa ggcgacctgc
    26701 attacggtca tggcgaagcg ggcactcacg caccgggcat gagccagcgt cggatgctgg
    26761 aagtcgaaat tgacgtgctg gacaaaaaca accgtctggc tgaacgcaac cgcgcgcgct
    26821 ttgctgcccg caagcaactg gtgctcaacc tggtttccag cccaggttcc ggtaaaacca
    26881 ccctgctgac ggaaacccta atgcgcctga aagacagcgt tccgtgcgcg gttattgaag
    26941 gtgaccagca aaccgtgaac gatgccgcac gcattcgcgc taccggcaca ccagcgattc
    27001 aggtgaacac aggtaaaggc tgccatcttg acgcacagat gattgccgac gccgcaccgc
    27061 gtctgccact ggacgataac ggtattctgt ttatcgaaaa cgttggcaac ctcgtatgcc
    27121 cggccagctt cgatctcggt gaaaaacaca aagtggcggt gctttccgtt accgaaggtg
    27181 aagacaaacc gctgaaatat ccgcatatgt ttgccgccgc ctcgctgatg ctgctcaaca
    27241 aagttgacct gttgccgtat ctcaactttg acgttgagaa gtgcatcgcc tacgcccgcg
    27301 aagtcaatcc agaaattgaa atcatcctta tttccgccac cagcggtgaa gggatggacc
    27361 agtggctgaa ctggctggag acacagcgat gtgcataggc gttcccggcc agatccgcac
    27421 cattgacggc aaccaggcaa aagtcgacgt ctgcggcatt cagcgcgatg tcgatttaac
    27481 gttagtcggc agctgcgatg aaaacggtca gccgcgcgtg ggccagtggg tactggtaca
    27541 cgttggcttt gccatgagcg taattaatga agccgaagca cgcgacactc tcgacgcctt
    27601 acaaaacatg tttgacgttg agccggatgt cggcgcgctg ttgtatggcg aggaaaaata
    27661 atgcgttttg ttgatgaata tcgcgcgccg gaacaggtga tgcagttaat tgagcatctg
    27721 cgcgaacgtg cttcacatct ctcttacacc gccgaacgcc ctctgcggat tatggaagtg
    27781 tgcggtggtc atacccacgc tatttttaaa ttcggcctcg accagttact gcctgaaaac
    27841 gttgagttta tccacggtcc gggttgcccg gtgtgcgtac tgccgatggg tagaatcgac
    27901 acctgcgtgg agattgccag ccatccggaa gtcatcttct gtacctttgg cgacgccatg
    27961 cgcgtaccag ggaaacaggg atcgctgttg caggcaaaag cacgcggtgc cgatgtgcgc
    28021 atcgtttact cgccgatgga tgcgttgaaa ctggcgcagg agaatccaac ccgcaaagtg
    28081 gtgttcttcg gcttaggttt tgaaaccacc atgccgacca ccgccatcac tctgcaacag
    28141 gcgaaagcgc gtgatgtgca gaatttttac ttcttctgcc agcacattac gcttatcccg
    28201 acgctgcgca gtttgctgga acagccggat aacggtatcg acgcgttcct cgcgccgggc
    28261 cacgttagta tggttatcgg cactgatgcc tataatttta tcgccagcga ttttcatcgt
    28321 ccgctggtgg tcgctggttt cgaacccctt gatctactgc aaggcgtggt catgctggtg
    28381 gagcagaaaa tagcggccca cagcaaggta gagaatcagt atcgtcgggt ggtaccggat
    28441 gccggtaacc tgctggcgca acaggcgatt gccgatgtgt tctgtgtcaa cggcgacagc
    28501 gaatggcgcg gcttaggcgt gattgaatct tctggcgtgc acctgacgcc ggattatcaa
    28561 cgattcgatg ccgaagcaca tttccgcccg gcaccgcagc aggtctgcga tgacccgcgc
    28621 gcgcgttgtg gtgaggtatt aacgggcaaa tgtaagccgc atcaatgccc gctgtttggt
    28681 aacacctgta atcctcaaac cgcatttggt gcactgatgg tctcctccga aggagcgtgc
    28741 gccgcgtggt atcagtatcg tcagcaggag aatgaagcgt gaataatatc caactcgccc
    28801 acggcagcgg cggccaggcg atgcagcaat taatcaacag cctgtttatg gaagcctttg
    28861 ccaacccgtg gctggcagag caggaagacc aggcacgtct tgatctggcg cagctggtag
    28921 cagaaggcga tcgtctggcg ttctccaccg acagttacgt cattgacccg ctgttctttc
    28981 ctggcggtaa tatcggcaag ctggcgattt gcggcaccgc gaatgacgtt gcggtcagtg
    29041 gcgctattcc gcgctatctc tcctgtggct ttatcctcga agaaggattg ccgatggaga
    29101 cactgaaagc cgtagtaacc agcatggcag aaaccgcccg cacggcaggc attgccatcg
    29161 ttactggcga tactaaagtg gtgcagcgcg gcgcggcaga taaactgttt atcaacaccg
    29221 ctggcatggg cgcaattccg gcgaatattc attggggcgc acagacgcta accgcaggcg
    29281 atatgttgct ggtgagcggc acgctcggcg accacggggc gactatcctt aacctgcgtg
    29341 agcagctggg gctggatggc gaactggtca gcgactgcgc ggtgctgacg ccgcttattc
    29401 agacgctgcg tgacattccc ggcgtgaaag cgctgcgtga tgccacccgt ggtggtgtaa
    29461 acgcggtggt tcatgagttc gcggcagcct gcggttgtgg tattgaaatt tcagaagcgg
    29521 cgctgccggt taaacctgcc gtgcgcggcg tttgcgaact gctgggactg gacgccctga
    29581 actttgccaa cgaaggcaaa ctggtgatcg ccgttgaacg caacgcggca gagcaagtgc
    29641 tggcagcgtt acattcccat ccactgggga aagatgcggc gctgattggt gaagtggtgg
    29701 aacgtaaagg tgttcgtctt gccggtctgt atggtgtgaa acgaaccctc gatttaccac
    29761 acgccgaacc gcttccgcgt atatgctaat aaaattctaa atctcctata gttagtcaat
    29821 gaccttttgc accgctttgc ggtgctttcc tggaagaaca aaatgtcata tacaccgatg
    29881 agtgatctcg gacagcaagg gttgttcgac atcactcgga cactattgca gcagcccgat
    29941 ctggcctcgc tgtgtgaggc tctttcgcaa ctggtaaagc gttctgcgct cgccgacaac
    30001 gcggctattg tgttgtggca agcgcagact caacgtgcgt cttattacgc gtcgcgtgaa
    30061 aaagacaccc ccattaaata tgaagacgaa actgttctgg cacacggtcc ggtacgcagc
    30121 attttgtcgc gccctgatac gctgcattgc agttacgaag aattttgtga aacctggccg
    30181 cagctggccg caggtgggct atacccaaaa tttggtcact attgcctgat gccactggcg
    30241 gcggaagggc atatttttgg tggctgtgaa tttattcgtt atgacgatcg cccctggagc
    30301 gaaaaagagt tcaatcgtct gcaaacattt acgcagatcg tttctgtcgt caccgaacaa
    30361 atccagagcc gcgtcgttaa caatgtcgac tatgagttgt tatgccggga acgcgataac
    30421 ttccgcatcc tggtcgccat caccaacgcg gtgctttccc gcctggatat ggacgaactg
    30481 gtcagcgaag tcgccaaaga aatccattac tatttcgaca ttgacgatat cagtatcgtc
    30541 ttacgcagcc accgtaaaaa caaactcaac atctactcca ctcactatct tgataaacag
    30601 catcccgccc acgaacagag cgaagtcgat gaagccggaa ccctcaccga acgcgtgttc
    30661 aaaagtaaag agatgctgct gatcaatctc cacgagcggg acgatttagc cccctatgaa
    30721 cgcatgttgt tcgacacctg gggcaaccag attcaaacct tgtgcctgtt accgctgatg
    30781 tctggcgaca ccatgctggg cgtgctgaaa ctggcgcaat gcgaagagaa agtgtttacc
    30841 actaccaatc tgaatttact gcgccagatt gccgaacgtg tggcaatcgc tgtcgataac
    30901 gccctcgcct atcaggaaat ccatcgtctg aaagaacggc tggttgatga aaacctcgcc
    30961 ctgaccgagc agctcaacaa tgttgatagt gaatttggcg agattattgg ccgcagcgaa
    31021 gccatgtaca gcgtgcttaa acaagttgaa atggtggcgc aaagtgacag taccgtgctg
    31081 atcctcggtg aaactggcac gggtaaagag ctgattgccc gtgcgatcca taatctcagt
    31141 gggcgtaata atcgccgcat ggtcaaaatg aactgcgcgg cgatgcctgc cggattgctg
    31201 gaaagcgatc tgtttggtca tgagcgtggg gcttttaccg gtgccagcgc ccagcgcatt
    31261 ggtcgttttg aactggcgga taaaagctcc ctgttcctcg acgaagtggg cgatatgcca
    31321 ctggagttac agccgaagtt gctgcgtgta ttgcaggaac aggagtttga acgtctcggc
    31381 agcaacaaaa tcattcagac ggacgtgcgt ctaatcgccg cgactaaccg cgatctgaaa
    31441 aaaatggtcg ccgaccgtga gttccgtagc gatctctatt accgcctgaa cgtattcccg
    31501 attcacctgc cgccactacg cgagcgtccg gaagatattc cgctgctggc gaaagccttt
    31561 accttcaaaa ttgcccgtcg tctggggcgc aatatcgaca gcattcctgc cgagacgctg
    31621 cgcaccttga gcaacatgga gtggccgggt aacgtacgcg aactggaaaa cgtcattgag
    31681 cgcgcggtat tgctaacacg cggtaacgtg ctgcagctgt cattgccaga tattgtttta
    31741 ccggaacctg aaacgccgcc tgccgcaacg gttgtcgccc aggagggcga agatgaatat
    31801 cagttgattg ttcgcgtgct gaaagaaacc aacggcgtgg ttgccgggcc taaaggcgct
    31861 gcgcaacgtc tggggctgaa acgcacgacc ctgctgtcac ggatgaagcg gctgggaatt
    31921 gataaatcgg cattgattta actgcaaatt gccggacaga tctgcctgtc cggcatacta
    31981 ttcacgaggt tttttcggac gatatttttc cggcagttct ggcaccggac gcttgtcatc
    32041 gatgagatga cgcacggtta agatcggatg acgccacagc attctcggcc cggcccaacg
    32101 cataatctgt ttcatctctt cacgctttgc aggctggtaa cagtgcaccg gacactgctt
    32161 acaggctggt ttctcttcgc cgaacacaca tttatccagc cgcttttgcg cgtaaacaaa
    32221 caacgcctcg taatgctccg gctccgctga cgcctgcggg catttcgctt gataaagatc
    32281 gatcattttt ttaatcgtca gtttttcacg agagatacgc ttgccggaca tgctgcctcc
    32341 acctcattaa gatgtattta tattacatct taatcttaaa gggcactatg actctaaaga
    32401 agaaaggttc gccaaccgac gcaattttgc gtacttgctt cataagcatc acgcaaaagc
    32461 tgcaaaacag catctttccc ggaaccagca tcaagaactc accgttcgct tcttcccctg
    32521 aaatgattaa ctccggtatc atgtgcgcct tatgtgatta caacgaaaat aaaaaccatc
    32581 acaccccatt taatatcagg gaaccggaca taaccccatg agtgcaatag aaaatttcga
    32641 cgcccatacg cccatgatgc agcagtatct caggctgaaa gcccagcatc ccgagatcct
    32701 gctgttttac cggatgggtg atttttatga actgttttat gacgacgcaa aacgcgcgtc
    32761 gcaactgctg gatatttcac tgaccaaacg cggtgcttcg gcgggagagc cgatcccgat
    32821 ggcggggatt ccctaccatg cggtggaaaa ctatctcgcc aaactggtga atcagggaga
    32881 gtccgttgcc atctgcgaac aaattggcga tccggcgacc agcaaaggtc cggttgagcg
    32941 caaagttgtg cgtatcgtta cgccaggcac catcagcgat gaagccctgt tacaggagcg
    33001 tcaggacaac ctgctggcgg ctatctggca ggacagcaaa ggtttcggct acgcgacgct
    33061 ggatatcagc tccgggcgtt ttcgcctgag cgaaccggcc gaccgcgaaa cgatggcggc
    33121 agagctgcaa cgcactaatc cggcggaact gctgtatgca gaagatttcg ccgagatgtc
    33181 gctgattgaa ggccgtcgcg gcctgcgccg tcgcccgctg tgggagtttg aaatcgacac
    33241 cgcgcgccag cagttgaatc tgcaatttgg cacccgcgat ctggtcggtt ttggcgtcga
    33301 gaacgcgccg cgcggacttt gtgctgccgg ttgtctgttg cagtatgcga aagataccca
    33361 acgcacgacc ctgccgcata ttcgttctat caccatggaa cgtgagcagg acagcatcat
    33421 tatggatgcc gcgacacgtc gtaacctgga aattactcag aacctggcgg gcggtgcgga
    33481 aaatacgctg gcttctgtgc tcgactgcac cgtcacgccg atgggtagtc gtatgctgaa
    33541 acgctggctg catatgccag tgcgcgatac ccgcgtgttg cttgagcgcc agcaaactat
    33601 tggcgcattg caggatttca ccgccgagtt gcagccggta ctgcgtcagg tcggcgacct
    33661 ggaacgtatt ctggcacgtc tggctttacg aactgctcgc ccacgcgatc tggcccgtat
    33721 gcgccacgct ttccagcaac tgccggagct gcgtgcgcag ttagaaactg tcgatagtgc
    33781 accggtacag gcgctacgtg agaagatggg cgagtttgcc gagctgcgcg atctgctgga
    33841 gcgagcaatc atcgacacac cgccggtgct ggtacgcgac ggtggtgtta tcgcatcggg
    33901 ctataacgaa gagctggatg agtggcgcgc gctggctgac ggcgcgaccg attatctgga
    33961 gcgtctggaa gtccgcgagc gtgaacgtac cggcctggac acgctgaaag ttggctttaa
    34021 tgcggtgcac ggctactaca ttcaaatcag ccgtgggcaa agccatctgg cacccatcaa
    34081 ctacatgcgt cgccagacgc tgaaaaacgc cgagcgctac atcattccag agctaaaaga
    34141 gtacgaagat aaagttctca cctcaaaagg caaagcactg gcactggaaa aacagcttta
    34201 tgaagagctg ttcgacctgc tgttgccgca tctggaagcg ttgcaacaga gcgcgagcgc
    34261 gctggcggaa ctcgacgtgc tggttaacct ggcggaacgg gcctataccc tgaactacac
    34321 ctgcccgacc ttcattgata aaccgggcat tcgcattacc gaaggtcgcc atccggtagt
    34381 tgaacaagta ctgaatgagc catttatcgc taacccgctg aatctgtcgc cgcagcgccg
    34441 tatgttgatt attaccggcc cgaacatggg cggtaaaagt acctatatgc gccagaccgc
    34501 actgattgcg ctgatggcct acatcggcag ctatgtaccg gcacaaaaag tcgagattgg
    34561 acctatcgat cgcatcttta cccgcgtagg cgcggcagat gacctggctt ccgggcgctc
    34621 aacctttatg gtggagatga ctgaaaccgc caatatttta cataacgcca ccgaatacag
    34681 tctggtgtta atggatgaga tcgggcgtgg aacgtccacc tacgatggtc tgtcgctggc
    34741 gtgggcgtgc gcagaaaatc tggcgaataa gattaaggca ttgacgctgt ttgccaccca
    34801 ctatttcgag ctgacccagc taccggagaa aatggaaggc gtcgccaacg tacatctcga
    34861 tgcgctggag cacggcgaca ctatcgcctt tatgcacagc gtgcaggacg gcgcggcgag
    34921 caaaagctac ggtctggcgg ttgcagctct ggcgggcgta ccgaaagagg ttattaagcg
    34981 cgcacggcaa aaactgcgtg agctggaaag catttcgccg aacgccgccg cgacgcaagt
    35041 ggatggtacg cagatgtctt tgctgtcagt gccagaagaa acttcgcctg cggtcgaagc
    35101 actggaaaat ctcgacccgg attcactgac tccgcgtcag gcgctggagt ggatttatcg
    35161 cttgaagagt ctggtgtaat aacaattccc gatagtcttt tgctatcggg aatattaacg
    35221 acaactgacg aatcaaataa aaataccctg tataatagga aagcttattt tacagggtaa
    35281 aaccatgcca tctacacgct atcaaaaaat caatgcccat cactatcgcc atatatgggt
    35341 cgttggtgat attcatggtg aatatcagtt attacaatcc cgtttacatc aactctcttt
    35401 ttaccctgaa accgacttac ttatttccac aggcgataat attgatcgtg ggccgaagag
    35461 tcttaacgtg ctgcgcctgc taaaccaacc ctggtttacg tcggttaaag gcaaccacga
    35521 agcgatggcg ttggatgcat tcgaaactgg cgatggaaat atgtggcttg ccagcggtgg
    35581 tgactggttt ttcgatttaa atgattcaga gcaacaagag gcaatagatc tgttgctgaa
    35641 attccatcac cttccacata ttattgaaat cactaacgac aacataaaat atgtcatcgc
    35701 acatgcagat tatccgggga gtgaatatct ctttggtaaa gaaatagcgg agagcgaatt
    35761 actctggcct gttgatcgtg tgcagaaatc gcttaatggc gagttacaac aaataaacgg
    35821 cgctgattat tttatatttg gacatatgat gtttgataac attcagacgt tcgctaacca
    35881 gatttatatt gataccggca cgccgaaaag cgggcggctg tcattttata aaataaagta
    35941 gtctcatgct tcttctgtga agcatgagta accaaatgtt attgcaggcc attattcatt
    36001 tttcgcaacc agcaaagaga gatcctgctt cgccagcgcg agactggcac tctccggcaa
    36061 accgtcgtct gtgataatct gatcaaactc gcttaatggt aacgccagcc atgtcgccac
    36121 ctgaccatat ttcgtcgcat cacagaccaa aactcgctgg cggctggcac tggcaatcgc
    36181 ccgtttcacc gtgactttat cttccgctgg cgtagaaatc ccccgcacac tccatgacga
    36241 tgcagaaata aaagcctgat caatcatcag gctgcgcagc atggtcgcag cggcttcccc
    36301 gacacaggaa cggttttccc gacacactgc accgccagtg tgaataattg tgcaattact
    36361 gttgtcgagc aagtagttcg caataacgaa atcgtttgtg accacagtca gtgactccat
    36421 gtgaatcaga tgctgtgcta tcgctaacgt ggtcgttccc gcatccagat agatacaact
    36481 tcccggctga acaagacttg ccgccagctt gccaatagcc gctttttgcg tcattgccag
    36541 cgcagttttt acctgatgag aaggttcatg cgccacgcgt cccggagact ggacgcctcc
    36601 ggacaccagc acaacggctc cctgctgctc cagtttttgt aaatcccgac gaatggtcat
    36661 atgtgacaca ttcattctgt ccgttagttc agcaatactg acaatgcctt tttcagctac
    36721 catctcaagg atgatttggc gacgctctac gggtatcaac ttttgctcct tcctttgtcc
    36781 tgctgacatt ctacgctatt tgcctgcgaa acgtgcgcgg cgcaactaac gcttagttca
    36841 cataaaataa cacacaatgt taatttatgt gaatcagctc accatatcgt tatcttccag
    36901 cgcttatatt cacaatatca aacaaaatat cacttaaatt aacaaggaga gcagatgaaa
    36961 acgggatctg agtttcatgt cggtatcgtt ggcttagggt caatgggaat gggagcagca
    37021 ctgtcatgtg tccgcgcagg tctttctacc tggggcgcag acctgaacag caatgcctgc
    37081 gctacgttga aagaggcagg tgcttgcggg gtttctgata acgccgcgac gtttgccgaa
    37141 aaactggacg ctctgctggt gctggtggtc aatgcggccc aggttaaaca ggtgctgttt
    37201 ggtgaaacag gcgttgcaca acatctgaaa cccggtacgg cagtaatggt ttcttccact
    37261 atcgctagtg ctgatgcgca agaaattgct accgctctgg ctggattcga tctggaaatg
    37321 ctggatgcgc cagtttctgg tggtgcagta aaagccgcta acggtgaaat gactgtcatg
    37381 gcctccggta gcgatattgc ctttgaacga ctggcacccg tgctggaagc cgttgccgga
    37441 aaagtttatc gcataggtgc agaaccggga ctaggttcga ccgtaaaaat tattcaccag
    37501 ttgttagcgg gcgtacatat tgctgccgga gccgaagcga tggcacttgc agcccgtgcg
    37561 gggatcccgc tggatgtgat gtatgacgtc gtgaccaatg ccgccggaaa ttcctggatg
    37621 ttcgaaaacc ggatgcgtca tgtggtggat ggcgattaca ccccgcattc agccgtcgat
    37681 atttttgtta aggatcttgg tctggttgcc gatacagcca aagccctgca cttcccgctg
    37741 ccattggcct caacagcatt gaatatgttc accagcgcca gtaacgcagg ttacgggaaa
    37801 gaagacgata gcgcagttat caagattttc tctggcatca ctctaccggg agcgaaatca
    37861 tgatcaagat tggcgttatc gccgatgatt ttaccggcgc gacggatatc gccagttttc
    37921 tggtggaaaa cggtctacca acggtacaaa ttaacggtgt tccaacaggt aaaatgccgg
    37981 aagcaatcga cgcactggtg atcagcctga aaacgcgctc ctgtccggtg gttgaagcca
    38041 cacagcaatc gctggcggct ctgagctggt tgcaacagca aggttgcaaa cagatctatt
    38101 tcaaatactg ctctactttc gacagtacgg cgaaaggtaa tatcggcccg gttaccgatg
    38161 cattaatgga tgctctcgac acgccgttta cggtcttctc tccggccctg ccggtcaacg
    38221 gacgtacggt ttatcagggg tatttgttcg taatgaatca actgctggcc gaatccggga
    38281 tgcgccatca cccggtaaat cccatgaccg acagctatct tccccgtctg gttgaagcgc
    38341 aatccacagg gcgctgcggc gtcgtttcgg cacatgtttt cgaacaaggt gtggatgccg
    38401 ttcgtcaaga gctggctcgc ttacagcaag agggctaccg ctacgcggtg cttgatgcgc
    38461 tgaccgaaca ccatctggaa attcagggag aagccttgcg cgatgcccca ctggtaacgg
    38521 gcggttctgg tctggcgatt ggcctggccc ggcagtgggc gcaagaaaac ggtaaccagg
    38581 ctcgcgaagc agggcatccg ctcgctgggc gcggcgtagt gctctccggt tcatgctctc
    38641 aaatgaccaa ccgccaggtg gcacattacc gtcaaattgc accagcccgt gaagttgatg
    38701 tggcacgctg cctctcaacc gaaactctgg ccgcttatgc acacgaactg gcagagtggg
    38761 ttctgggcca ggaaagtgta cttgctccac tggtttttgc caccgccagc actgacgcat
    38821 tggcagcaat tcaacagcaa tacggtgcac aaaaagccag tcaggcagta gaaactctat
    38881 tttctcaact agcggcgcgg ttagcagcgg aaggcgtgac acgctttatt gtcgcaggcg
    38941 gtgagacctc cggcgtagtc acacagagcc tgggcataaa agggtttcat attggcccaa
    39001 ccatttcccc cggcgtgccg tgggtaaacg cactggataa gcctgtctca ctcgccctta
    39061 aatctggcaa cttcggtgat gaagcctttt tttcacgagc ccaaagagag tttttatcat
    39121 gagcgatttc gcaaaagtag agcagtcttt gcgagaggag atgacgcgga ttgccagttc
    39181 attctttcag cgcggctatg caaccggttc ggctggcaat ctgtcgctgc ttttacctga
    39241 cgggaattta ctggcaacac cgacaggttc atgcctgggc aatctcgatc cgcagcggct
    39301 ttccaaagtc gccgcggatg gcgaatggtt aagtggtgac aaaccctcga aagaggtgct
    39361 ctttcatctg gcgctgtatc gcaacaatcc gcgctgtaaa gcggtggtgc atttgcacag
    39421 cacatggtcg acggcgcttt cctgcctgca agggctggac agcagcaacg ttattcgtcc
    39481 gttcacacca tacgtggtga tgcggatggg aaatgtcccg ctggtgcctt attaccgacc
    39541 gggcgataaa cgcatcgcac aggatctggc ggaactggca gcagacaatc aggctttttt
    39601 actggcaaat catggcccag tggtttgcgg tgaaagcctg caagaagccg ccaacaatat
    39661 ggaagagctg gaggaaacgg caaagctgat ttttattctc ggtgaccgcc cgatccgtta
    39721 tctgaccgca ggtgaaattg cggaattaag gagttaatgc aatgcctcgt tttgcagcta
    39781 atttatccat gatgttcacc gaagtgcctt ttattgaacg cttcgccgca gcgcgaaaag
    39841 ccgggttcga tgctgtggaa tttctgtttc cctatgacta ctccaccctg caaatccaaa
    39901 agcaactgga gcaaaaccat ctgacactgg cgctgttcaa taccgctccc ggagatatta
    39961 atgcggggga atggggatta tccgcccttc ccggacgtga gcacgaagca cacgcagaca
    40021 tcgacctggc actggaatat gcattggcgc tcaattgtga acaagtccat gtgatggcag
    40081 gcgtcgtgcc cgctggcgaa gacgctgaac ggtaccgggc agtatttatc gataatctcc
    40141 gctacgctgc cgaccgcttt gcgctacacg gcaagcgaat attagttgaa gcactcagcc
    40201 ccgacgttaa gcctcattat ctcttctcca gccagtatca ggcactggct attgttgaag
    40261 aggttgcgcg agataacgtg tttattcaac tagatacttt tcatgcgcaa aaagtagatg
    40321 gcaacctgac gcatttaatc cgcgactacg ctgggaaata tgcgcacgta caaattgccg
    40381 gactacctga tcgacatgaa ccggacgatg gagaaatcaa ctatccgtgg ctgttccgcc
    40441 tgttcgatga ggtgggatat cagggctgga tcggttgtga atataaacct cgtggcctca
    40501 ccgaagaagg gcttggctgg tttgacgcct ggcgctaatt cgttaaatcc ctaattacaa
    40561 cgtacccata catcccccca tctacggatg gggtaggtta actataactt tcagacaggg
    40621 tttcccatgt ccacaattac attgttatgc attgcgttag ctggcgtaat catgctgttg
    40681 ctgctggtca tcaaggcaaa ggtacaacca ttcgttgctc tgctcctcgt cagcctgtta
    40741 gtcgcacttg cggcgggtat accggcgggc gaagtgggta aagtgatgat cgccgggatg
    40801 ggcggtgtgc ttggttccgt cactattatt attggtctgg gcgctatgct ggggcgtatg
    40861 atcgaacact ctggtggtgc agagtcactg gctaattatt tcagtcgcaa gttaggtgac
    40921 aaacgaacta tcgctgcgct gactctggca gcgttcttcc tcggtattcc cgtcttcttt
    40981 gatgtcggct ttattattct tgcgccaatc atttacggtt ttgccaaggt tgccaaaata
    41041 tcgccactca aatttggcct gcctgtcgct ggaatcatgc taactgttca tgtggcggta
    41101 ccgccgcatc caggccctgt cgccgcagcg gggttactcc acgcagacat cggctggcta
    41161 accatcatcg gtattgcgat ttctattccc gtagggattg ttggctactt tgcagcgaaa
    41221 ataatcaata cgcgccaata tgcgatgtcg gtggaagtac tggaacagat gcaactggct
    41281 ccggccagtg aggaaggcgc aacaaaatta agcgataaaa taaatccacc gggcgtcgcg
    41341 ctggtcacct cgctaattgt tattcctatc gcgattatca tggcgggtac ggtttccgca
    41401 acactgatgc cgccttcgca tcccctgctt ggtacgctac agctgatcgg ctcaccaatg
    41461 gtagctctaa tgattgcgct ggtgctggca ttctggttat tggctttacg tcgcggctgg
    41521 agcttacaac acaccagcga cattatgggc tcagcccttc ccactgcggc ggtagtgatt
    41581 ttggttactg gtgctggagg ggtatttggc aaagtgctgg tggaatcggg cgtcggcaaa
    41641 gcccttgcca atatgctaca aatgattgac ctgcctctgt taccagccgc atttattatt
    41701 tcactggctc tgcgtgcatc gcaggggtca gcaaccgtag caatcctgac aaccggcggg
    41761 ttactctcag aagcggtgat gggattgaat ccgattcagt gcgtattggt gacgctggca
    41821 gcctgctttg gtgggcttgg cgcgtcacat attaatgact cagggttctg gattgtgacc
    41881 aaatatctgg ggttgtctgt agcagacggt ctgaaaacct ggacagtgtt aacgaccatt
    41941 ctcggtttta ccggattctt aattacctgg tgcgtatggg cggtaatttg accttaataa
    42001 aaaggtccga tgggcatcgg accttttatt taacgcttac cttccaccag cagcggcggg
    42061 atgctcggca ccattggcgc gtcatcaata tctttctgcg tcatgcggaa cgcttcggga
    42121 taatgttcgc ggctggtacg gcgcagcggt tcggtatcgc gccaggtata aaggcaatgc
    42181 tggcactgat ataccgtcca gacatctttc accggcgatt tcgccatcac ttcaatctgt
    42241 tcatcggcac aacgtggaca aatcatcttc ttctccttat ttacgtgcag ccagcatagc
    42301 ggtcagtttt tcagcccagg ctttggtttc cggtaaatcc accaccggtt ggctgtagtg
    42361 accacggttg tccggggcga caggcgtagt ggcgtcgata atcagcttgt cggtgatccc
    42421 cgcagggctt gagcctggat cgagttccag cacggacata ttcggcaact gcaccaaatc
    42481 ccctgccggg ttcactttcg aggagagcgc ccacatcacc tgcggcaggt tgaacgggtc
    42541 aacgtcttca tcgaccataa tcaccatctt cacgtagccc agaccatgcg gcgtggtcat
    42601 tgcgcgcagg cccaccgcgc gggcaaagcc gccgtagcgt tttttggtgg agataatcgc
    42661 cagcaggcca tgggtgtaca tggcgtttac cgcctgcact tcagggaact cggctttcag
    42721 ctgctgatac agcggcacgc aggtggctgg ccccatcagg tagtcgattt cggtccacgg
    42781 cataccgagg tacagcgatt cgaaaatcgg cctggtgcgg taagagactt tatcgatgcg
    42841 caccacggtc atgttacgcc cgccggagta gtgcccggta aactcaccga acggcccttc
    42901 gatttcgcgt ttacggcttt cgatgacccc ttcgaggatc acttctgaac cccacggcac
    42961 atcaaaaccg gtcaacgggg cggtggcgat cgggtacggg ctttcacgca gcgcgcctgc
    43021 catttcgtac tcggactgat catatttcag cggcgtggcc cccatcagcg tgatgatcgg
    43081 atcgttaccg agcgtgatcg caatcggcag atcttcaccg cgctcttctg ctttatgcag
    43141 atgcagggcg atatcgtgca tcggcaccgg ttgcaggccg agcttacgct tgcccttcac
    43201 ttccatgcgg tagatgccga cgttctgctt gccgaagtta tccgggtcga gcggatcgcg
    43261 ggaaaccacg cacgctttgt cgagatagaa accgccatcg ccatcgttta aacgaaacag
    43321 cggcaggata tcgaacaggt tgatctcgtc gccatcaacg gtgttctgcg cccaggctgg
    43381 attggcgcgg cgctccgggg caatcgggaa gttatcccag cggcggataa actcatcaat
    43441 ctgcttttta accggggcat ttggcggcag gccgagggaa atcgcgtggt tctgccagga
    43501 accgatggtg ttcatcgcca cgcgggcatc ggtaaagccg cgaatattat caaaccacag
    43561 cgcgggcgcg ccgtcgccga tacgcccggt ggcgttagct gctgctgcca gatccggctc
    43621 ggcgttcact tcttcgctga ttttcagtaa ctggccgtgg tcatcaagcg cctgtaaaaa
    43681 gctgcgtaaa tcatcaaatg ccattattca ttctcctgag aaaaattccg ggcctgcggc
    43741 aatccttgcc agcgcctggc gtggggatgt tcgaggccaa attgatccag cacgcgggct
    43801 accacatggt ggacaatgtc atctaccgtt tcgggatggt tataaaaggc aggcatcggc
    43861 ggcaccatcg ccacgcccat gcgtgaaagt gcgagcatat tttcgagatg gatggtgcta
    43921 agcggcattt cacgcggcac cagcaccagt ttgcggcctt ctttgagcac gacgtccgcc
    43981 gcgcgcccta ccaggccatc ggcgtaacca gcgcggatac cggcgagcgt tttcatactg
    44041 cacggaataa cgatcatgcc gtcggtacga aaagaaccgg atgagatgat cgccgcctga
    44101 tccgccgggt tatggctgaa gtctgcgagg gcagcaacat cgcgagcgct gtaaggcgtt
    44161 tccagttcaa tggtggtttt cgcccacttc gacatcacca gatgagtctc gacattcggc
    44221 atctcccgca gcgcttgcag taatgccaca ccaagaggcg caccggtagc ccctgtcatc
    44281 ccgacgatca gtttcattgc ttatcctctt aattgttcgt atacgaacgt tattattaac
    44341 atactccttc atttccacag cggcaaggat tgaaaagtaa caacatcttc ttcgtcacta
    44401 aaaaaccgct atgatagcga cagagaatta gcccggagtt tacatggcgt tacgaaataa
    44461 agcgttccat cagttacgac agctttttca gcagcacacg gctcgctggc agcacgagtt
    44521 acctgacctg accaaaccac agtacgcggt gatgcgcgcc attgctgata aacctggtat
    44581 cgaacaggtg gcactgatag aagcagctgt cagcaccaaa gcaacgttag cagaaatgtt
    44641 ggcaagaatg gaaaatcgtg gcttagtcag acgtgaacat gaccccgctg ataagcggcg
    44701 acgctttgtc tggttgactg ccgaaggcga aaagatactt gctgcggcga tacccattgg
    44761 tgacagcgtg gatgaggaat ttttggggcg tttgagtgga gaagagcagg agctgtttat
    44821 gcagctggtg cgcaagatga tgaacgcata gggggcaaat ttccggatgc gacgctatcg
    44881 cgtcttatcc agactacaaa tacaccgact caccaaccga aagaaaaaag gccagcctcg
    44941 cttgagactg gcctttctga cagatgctta cttactcgcg gaacagcgct tcgatattca
    45001 gcccctgcgt ttgcaggatt tcgcgcaaac ggcgcaggcc ttcaacctga atctggcgaa
    45061 cacgttcacg ggtgaggcca atttcacgac ctacatcttc cagtgttgcc gcttcgtacc
    45121 ccagcaaacc gaatcgacgt gccagcactt cacgctgttt ggcgttcagc tcgaacagcc
    45181 atttgacgat gctctgcttc atatcgtcat cttgcgtggt atcttccgga ccgttctctt
    45241 tttcatcggc caggatgtcc agcaacgctt tttcggaatc accacccagc ggggtgtcta
    45301 ccgaggtaat gcgctcgtta agacgaagca tacggctgac gtcatcaact ggcttatcca
    45361 gttgctctgc gatctcttcc gcacttggtt catggtccag cttatgggac aactcacgtg
    45421 cggttcgcag gtaaacgttc agctccttta cgatgtgaat cggcaaacga atagtacggg
    45481 tttggttcat aatcgcccgt tcaatcgtct ggcgaatcca ccaggttgcg tatgttgaga
    45541 agcggaaacc acgttccggg tcaaacttct ctaccgcgcg gatcagcccc aggttgccct
    45601 cttcgataag gtccagcaac gccagaccac gattgccata acggcgggca atttttacca
    45661 ccagacgcaa gttactctcg atcatccggc ggcgagaggc gacatctcca cgcagtgcgc
    45721 gacgcgcaaa ataaacttct tcttcggccg ttaacagtgg tgaataacca atctcaccaa
    45781 ggtaaagctg agtcgcgtcc aacacacgct gtgtggctcc ctgcgataac agttcctctt
    45841 cggccaaatc gttatcactg ggttcctctt ctactaaggc cttttcgtca aaaacctcaa
    45901 ctccgttctc atcaaattcc gcatcttcat ttaaatcatg aactttcagc gtattctgac
    45961 tcataaggtg gctcctaccc gtgatccctt gacggaacat tcaagcaaaa gcctggttcc
    46021 gccgatttat cgctgcggca aataacgcag cgggtttacg gatttcccct tgtaacgaat
    46081 ttcaaaatgc aagcgtgttg aactggttcc ggtgctaccc atggttgcta ttttttgccc
    46141 cgccttcact tcttgttgtt cccggaccag cattgtgtcg ttatgggcgt aggcactcag
    46201 gtaatcatca ttatgtttga tgataatcag attaccgtag ccgcgcagcg cgttaccagc
    46261 ataaacaacg cggccatctg cggtcgcgat aattgcctgt cctttgctgc ctgcgatatc
    46321 aatccccttg ttgcccccct cagaagcgcc aaaggtttcg atcactttgc cctcagtcgg
    46381 ccagcgccag gtggagatag gcgtactggt tgatgtactg ctgacagttg gctcggttgt
    46441 gcttgctgtt ggtaccgtta caggcgctgt gaccgtggtc gcagctggct tgttgttcgg
    46501 caacattttg ttagcactct gttcacccga agattcagaa tacgtaattg tcggttgcga
    46561 cgcaacagca acggtggaat tttgtgcagg cttgatcaca actccttgct ctgctgcgtc
    46621 ggcctgggta atggcatttc cgccagtgat tggcgtaccg gaagcattac ccacctgcaa
    46681 ggtctgacca acgttcagcg cgtatggtgc ctgaatattg ttgcgctgag caaggtcacg
    46741 gaaatcgttg ccagtaatcc aggcgatata gaaaagtgtg tcgccttttt tcacggtata
    46801 ggtactgccg ctataactgc ctttcggaat gttcccatac tgacggttat agacgatgcg
    46861 tccgttttcc atctgtaccg gctgctgagc tactggctgc actggctgga tttgcggttg
    46921 ttgagtagcc tgaatttgtg gctgctgtac cggctgaatt tgcggttgct gggctgtaga
    46981 cgtcgtcccc attttcggcg gcggcgtaat caacatacca gaattggtat ttgcaggcgc
    47041 attgccatta acggagctga ccggtgccgg tggatttgaa gtgtcagaac agcctgccag
    47101 ccatagcgaa accagtgaca aagccgcaat gcggcgaacg gtgaattttg ggcttcccgc
    47161 gctcatttat cccccaggaa aaattggtta ataaccagtg acataattac cgtgcaaggc
    47221 accctactga acactggaaa agatgttcac gatacgctga cctgcggcaa aataaccagg
    47281 aaaaatccag gtatttcctc acgttttaag ccagctcacc cttcactaaa gggacaaagc
    47341 gcacggcctc cacggtatcg ataataaatt cgcctccccg acgacgcacc cgtttcaaat
    47401 actggtgctc ctcccctacg ggtaagacga gaatcccgcc ttcgtccagc tgcgtcatta
    47461 gcgcagttgg aatttccggc ggtgccgccg taacaatgat agcgtcaaac ggcgcgcgtg
    47521 cctgccaacc ttgccatcca tcgccatgac gggttgaaac attatgtaaa tcaagatttt
    47581 tcaggcggcg acgcgcctgc cactgcaaac ctttaatccg ttcgaccgag caaacatgct
    47641 ggacaagatg cgccaggatt gccgtttgat atcccgaacc agtgccaatt tccagcaccc
    47701 gcgactgcgg cgtcagctcg agtaattcgg tcattcgcgc caccatatac ggctgcgaaa
    47761 ttgtctgccc ctgacctatc ggcaaggcga tattgtccca ggctttttgt tcaaacgctt
    47821 catcaacgaa tttttcacgc ggcacggcgg caagtgcatt cagcacctgc tcatcctgaa
    47881 taccttgcgc acgtaattga tccagaagtg cttgtacgcg tctgcttacc attgcgtgcc
    47941 aactcccacg ctgtttaacc agtctgaaac cacatcttgc gcgctatgcg cagttaaatc
    48001 cacatgcagc ggcgtgatgg agacatagcc ctcatctacc gcagcaaaat cggtccccgg
    48061 accggcatca catttaccgc ccggcgggcc aatccagtac agcgtattgc cgcgcggatc
    48121 ttgctgcggg atcacctgat ctgccggatg tcgtgtaccg cagcgcgtca cgcgaatacc
    48181 tttgatttga tccaagggta aatccggaac gttaatatta agaatacgcc cggtgcgcag
    48241 cggctcttta cacagtgcgc gcaaaattga acaggttacc gccgcggcag tgtcgtaatg
    48301 tttatgcccg tcaagcgaga cggcaagcgc cggaaaacct aaatgacggc cttccatcgc
    48361 ggcggctacc gtaccggaat aaataacatc atcccccaga ttcggcccgg cgttaattcc
    48421 ggacacaaca atgtccgggc gcggacgcat cagagcattc acgccaagat agacgcaatc
    48481 ggtcggggtt cccatttgca cagcaatatc accattttca aaggtaaacg tgcgcaggga
    48541 ggattccagt gtcagagaat ttgaagcgcc gctgcggtta cgatcggggg cgaccacctg
    48601 aacgtcagca aactcacgca aggctttcgc cagcgtttgt ataccgggtg catgtacccc
    48661 gtcatcatta ctcagcaata tgcgcataat cacctgttgt gttgataagt tccctgacaa
    48721 cgctggttgc aaaactaccc gccggaagcc agaaacggat ctctacggtg acgtcatccc
    48781 accaattcca gcttaattgt tgcggataca gcagcatcgc tctgcgcgcg gcttcaactt
    48841 tttcgcgcac cagtaaagct tgtaattcag tttctgcggc gacagctgct tgttcgaatg
    48901 ccagcgcttc acgctgagtt ccccattcgc cactgcccgg caatgcggcg gttatcatca
    48961 gctctttatc gttgacgcga cgctgtaatt ccgccagttc ttcggtggtt gcgacaaacc
    49021 agctaccacg tccggctaat tgtagcgcat cgccgtcaac aacttgatta acgtctgctt
    49081 ttttgaggcg ctcagcaaca atctgattaa acaacgcact gcgggctgcc gacaaccaaa
    49141 aactccgttt attgcgatcg cgcaccggag tattggtttg cgcccagcgc tgcgccccct
    49201 gcaagttgct accgccaatc ccaaaacgtt gggcaccgaa gtagttcggt acacctttta
    49261 cgcaaatatc gatcagacgt tgttcaacgt catcgcgatt gctcacttcg cgcagaacca
    49321 gggtaaaggc gttacctttc agcgcgccta aacgcagctt gcgcttgtgc cgcgcatact
    49381 ccagcacctg gcagccttcc agttgaaagg cgctcagatc gggcatttcc ttgcctggca
    49441 cgcgagcgca taaccactgt tccgtaacag catgtttgtc tttttgccca gcgaagctga
    49501 cttcacgggc atgaattttc aggaatttcg ccagtgcatc cgccacaaaa cgggtattgc
    49561 agccgttttt gaggattcta accagaatat gctcaccttc accatcaggc tcaaagccca
    49621 aatcttccac caccacaaag tcttccggat tggctttcag cagcccggtg ccttgcggtt
    49681 taccgtggag gtaagtgaga ttatcaaact caatcatttt gttgccttaa tgagtagcgc
    49741 caccgcttca caggcaatcc cttccccacg tccggtaaat ccaagttttt ccgtagtagt
    49801 ggctttcacg ttaacatcat ccatatggca gccgagatct tcggcaataa acacgcgcat
    49861 ttgtggaatg tgcggcaaca tcttcggtgc ctgagcgatg atagtgacat cgacgttgcc
    49921 cagggtataa cccttcgcct gaatacgacg ccaggcttcg cgtagcagct cgcggctgtc
    49981 ggcaccttta aatgccggat cggtatccgg gaacagcttg ccgatatccc ccagcgccgc
    50041 cgcgccaagc aatgcatcgg tcaacgcatg gagcgccacg tcgccatcag aatgcgccag
    50101 caatcctttt tcgtaaggaa tgcgtacgcc accaatgata attgggcctt caccgccaaa
    50161 ggcatgtacg tcaaaaccgt gtccaattcg cattatgtat tctcctgatg gatggttcgg
    50221 gtgaggtaaa actcggccag tgccaaatct tccgggcgcg tgactttaat gttatccgca
    50281 cggccttcga ccaactgagg atggaatccg caatattcca gcgccgaggc ttcgtcggta
    50341 atagtcgcgc cttcatttag agcgcgcgtc agacagtcat gtaacagctc acgagggaaa
    50401 aattgcggcg tcagcgcgtg ccataagccg ttgcgatcaa cggtatgagc aatggcattt
    50461 ttgcccggtt cggcacgttt catcgtatcg cgcactggtg cggctaggat ccctcccgtg
    50521 cggctggttt cgctcaacgc caacaatcgc gcgaggtcat cctgatgcag acaaggacga
    50581 gcggcgtcat gcaccaatac ccactgcgcg tcgccagcgg ctttcaaacc tgccagcacg
    50641 gaatcggcac gctcatcacc gccatctaca acggtgattt gcggatgatt cgccagagga
    50701 agttgtgcaa aacggctatc gccaggactt atggcaatga cgacacgttt cacccgggga
    50761 tgcgccagca gcgcatgcac cgagtgttca agaatggttt gattaccgat tgagagatat
    50821 tgcttaggac attccgtttg cattcgacgg ccaaatccgg ccgccggaac cacggcgcaa
    50881 acatccaaat gagtggttgc catgttaatt cccgggctga tttatcgatt gttttgcccc
    50941 gcagactgtg cgcgcttcga cgcgtcaggc accagacgat aaaaagtttc gcccggcctg
    51001 gtcatgctga gttcattacg cgcacgctct tcgagcgcct cctggccgcc attgagatcg
    51061 tcaatttcgg caaaaagttg atcgtttcgc gctttaagtt tcgcatttgt agcttgctgt
    51121 gccgccacat catcattgac gcgggtatag tcatgtatac cgttcttacc gaaccacagc
    51181 gaatactgta gccagaccag aatagccagc aacagcagcg ttagtttacc catcctgccc
    51241 cctgaaaaac ggcatcatca tcccatgcat ccgaagacga ctctacatcc tctgttgggg
    51301 ataccgcgac aacgcgggca aatgtaccac atttgtccat tgttacgtat acccagggcg
    51361 tgcagaacat aatctcatta ttagttacgg tttgaattat gaacagagga gacgggaaag
    51421 tacaaattag cccagtagcc acataaacag tgcgccaaac ataatgccta ctgtcaccag
    51481 ggtgaaaaca atactgtagc gtagctttcc gtccatcaat gaatgcagcg caatccccac
    51541 cactaccgcg acgggcatca gcgccagaaa gaaaggccag gtgtagataa agaagaacag
    51601 cgtgttagag ccataaatca acatcggcat cgccagcgca aataaccagg agataaaacc
    51661 gaccacggca ccaggcagtg accatgtggt ttcttcatcc tcagtaaggc tgtcgttatt
    51721 tgttagtgta atgttatggc tattacgcat atttgatcct gttactttga cgaaccgggc
    51781 atggaaaccc ggtggtgtct caggatctga taatatcgtt ctgtctcaac agatctaata
    51841 attgctgtac caaatttgtt actaattgtt caccattgag atgaatttct gccgattcag
    51901 gcgcttcgta aacggaatct attcccgtaa agttgcgcag ttcaccggca cgcgctttct
    51961 tatataagcc ttttggatcg cgggcttcgc aaatcgccag cggcgtatcg acaaacactt
    52021 cgataaagcg cccttctcct acgcgttcgc gaaccatctg gcgttcggcg cggtgtggcg
    52081 agataaatgc ggtcagcact accagtccgg cttcaaccat caaattcgcc acttcaccga
    52141 cgcgacggat attctcttta cgatcggcat cgctaaaacc gagatcgctg cataatccgt
    52201 ggcgaacatt atcgccatcc agcagatacg tactgacgcc gagtttatgt aacgcctcct
    52261 ccagcgcccc ggcgaccgtt gatttaccgg acccggagag gccggtaaac cacagcacta
    52321 caccacgatg accgtggtgt agctcgcgtt gttgcacagt gaccggatgg ctatgccaga
    52381 cgacgttttc gtcatgcagc gccattattt atcccccagc aaatcgcgcg cgccccagtg
    52441 cggaaagtgg cgacgaacca gagcattcaa ttccagttcg aatgcactga attcagatgg
    52501 cgcagcagtt gcctggctaa ctggctcgtg caccataccg gcacccacgg tcacattgct
    52561 caggcgatcg ataaaaatca gcccacccgt caccggattt tgttgataac gatctaacac
    52621 cagcggctcg tcaaaagtga gatccacgag gccgatccca ttcagtggca ggttttcaac
    52681 ttcacgctgg gtaaggttat taatatcaac ctgatagcga atgccatcaa cacgcgcgcg
    52741 cgtcttctta ccggcaattt tgatgtcgta actctgccct ggagaaagcg gctgttccgc
    52801 catccatacc acatccaccg acgcgctctg caccgccggt aacgcttcgt ctgccgccag
    52861 cagcagatcg ccacggctga tgtcgatctc atccgtcagc accagggtga tcgcttctac
    52921 ggcaaaggct tcttcgcgat caccatcaaa agtcacgatc cgcgcgacgt ttgattccac
    52981 accagaaggc agcactttta cacgttgccc gacttccacg cgaccggatg ccagcgttcc
    53041 ggcgtaacca cgaaaatcga gattcgggcg attaacgtac tgcaccggga agcgcattgg
    53101 ctgagcatcc accactcgct ggatctccac ggtttccagc acttcgagca gtgtcggacc
    53161 gctgtaccac ggcatacttt cactttgcga agccacgttg tcgccttcca gcgcagagag
    53221 cggcacaaag cgaatatcca gattgccagg taactgttcg gcgaaagtca ggtagtcttt
    53281 acgaatgcgg gtgaatgtct cttcactgta atccaccaga tccattttgt tgatcgctac
    53341 gaccagatgt ttgatcccca gcagcgtgga gataaaactg tgacgacggg tttgatcgag
    53401 cacgccttta cgggcatcga tcagtaagat cgccagctca cacgttgagg cgccggtcgc
    53461 catattgcgg gtatactgct cgtgcccggg ggtgtcggcg ataataaatt tacgcttctc
    53521 ggtagagaaa tagcggtagg ccacatcaat agtgatgccc tgttcgcgct cagcttgcag
    53581 gccgtccacc agcagagcca gatccagttt ttcgccctgg gtgccgtgac gcttactgtc
    53641 gttatgcagc gatgagagct gatcttcgta gatttggcgg gtatcgtgca gcaggcgacc
    53701 aatcagggta cttttgccgt catcgacgct accacaggtc agaaaacgca gcaggctttt
    53761 atgttgttgc gcaatcatcc aggcttcgac gccgccttca ttggcgattt gttgtgcaag
    53821 tgcggtgttc atcttaaaaa tacccctgac gttttttcag ctccatagac cccgcctggt
    53881 cgcggtcaat cacgcggccc tgacgttcac tggtggtgga aaccagcatc tcttcgatga
    53941 tctccggcag tgtttgtgca tttgactcca ccgcaccggt cagcggccag cagcccagcg
    54001 tacggaaacg caccatccgt tttttaatca cttcgcccgg ttgcaggtcg atacggttgt
    54061 catcaatcat catcaacata ccgtcgcgtt ccagaaccgg acgttccgca gcgagataca
    54121 gcggcacaat gtcgatattt tccagccaga tgtattgcca gatatcctgc tcggtccagt
    54181 tagagagcgg gaaaacgcgg atgctttcgc ctttgttaat ctgcccgtta tagttgtgcc
    54241 acagctccgg gcgctgattt ttcgggtccc agcgatggaa gcgatcacgg aaagagtaga
    54301 tacgctcttt agcacgggat ttctcttcgt cgcggcgcgc accaccgaag gcggcatcaa
    54361 aaccgtattt attcagcgcc tgcttcaggc cttcggtctt cataatatcg gtatgtttcg
    54421 cgctgccgtg cacgaatgga ttaatcccca tcgccacgcc ttccgggttt ttatgcacca
    54481 gcagctcgca gccgtaggct ttcgccgtac gatcgcggaa ctcatacatc tcgcggaatt
    54541 tccagccggt atcgacatgc agcaagggga aaggcagcgt acctggataa aacgccttgc
    54601 gcgccaaatg cagcatgacg ctggaatctt taccgataga gtagagcatc accggatttg
    54661 agaattctgc cgccacctcg cgaataatgt ggatgctttc cgcctccagt tgccgcaggt
    54721 gagtaagtcg tttttgatcc ataaccgttc ctttgcaata ccgctatttt cttgccatca
    54781 gatgtttcga ctatagggag cgtaagagaa cgaatgaaat taccaattag aatgagtagt
    54841 tccttaacgg aataacgatt tggcaaagct aatatcaaaa agtgcttaag gcaccggatt
    54901 tcgggcgttt aggaagattt gaaattgttt tagcgcagcg gcagtttcat actatggcgg
    54961 taaaaaaatt tgcatggtat ttaaggactc actatgtttt ccgcattgcg ccaccgtacc
    55021 gctgccctgg cgctcggcgt atgctttatt ctccccgtac acgcctcgtc acctaaacct
    55081 ggcgattttg ctaataccca ggcacgacat attgctactt tctttccggg acgcatgacc
    55141 ggaactccag cagaaatgtt atctgccgat tatattcgcc aacagtttca gcaaatgggt
    55201 tatcgcagtg atattcggac atttaatagt cggtatattt ataccgcccg cgataatcgc
    55261 aagaactggc ataacgtgac aggaagtacg gtgattgccg ctcatgaagg caaagcgccg
    55321 cagcagatca tcattatggc gcatctggat acttacgccc cgctgagcga tgctgacgcc
    55381 gatgccaatc tcggcgggct gacgttacaa ggaatggatg ataacgccgc aggtttaggt
    55441 gtcatgctgg aattggcaga acgcctgaaa aatacgccta ccgagtatgg tattcgattt
    55501 gtggcgacca gcggcgaaga ggaagggaaa ttaggcgctg agaatttact caagcggatg
    55561 agtgacaccg aaaagaaaaa tacgctgctg gtgattaatc tcgataactt aattgttggc
    55621 gataaattgt atttcaacag cggtgtaaaa acccctgaag cagtaaggaa attaacgcgc
    55681 gacagggcgc tggcaattgc gcgcagtcac ggaatagccg caacgaccaa tccgggtttg
    55741 aataaaaatt atccgaaagg cactgggtgt tgtaatgacg cagaaatatt cgacaaagcg
    55801 ggcattgctg tactttcggt ggaagcgact aactggaatc ttgggaataa ggatggttat
    55861 cagcaacgcg caaaaacagc cgcattccct gcgggaaata gctggcatga cgtaagactg
    55921 gataatcaac agcatattga taaggcactt cctggaagaa tagaacgtcg ctgccgtgac
    55981 gttatgcgga taatgctacc gttggtgaag gagctggcga aggcgtcttg atgggtttga
    56041 aaatgggagc tgggagttct accgcagggg cggggaattc taagtgatat ccatcatcgc
    56101 atccagtgcg cccggtttat ccccgctggc gcggggaaca caaaaccaaa cttctccata
    56161 aattccatag ccgcggttta tccccgctgg cgcggggaac acacgcgcgt accggatcgc
    56221 ggacaacaaa ttgccggttt atccccgctg gcgcggggaa cacgcagtgg tgaggccggg
    56281 aagcgcggtt gagtccggtt tatccccgct ggcgcgggga acacggcaac ataacgaaca
    56341 aaatcaacgt caacctcggt ttatccccgc tggcgcgggg aacacttgct gaaaaagaag
    56401 gctccggcgt tatcagtcgg tttatccccg ctggcgcggg gaacacgcat ccatgccgac
    56461 gcctttacgt gtgcggggcg gtttatcccc gctggcgcgg ggaacacacg gtccatggca
    56521 cacaggatcc agacgttaac ggtttatccc cgctggcgcg gggaacacac taagcataca
    56581 taactgtttt taaacaaatt tattccgcat caacaatcta ccaactaaat taaaacattt
    56641 ccttattttt aaagaacgga taacttattg attatcaaca ggaagaaaag aaaccaaacg
    56701 taacccatcc aaatccaccg gaatacgtct gttttctccc caggtctgaa attcaaaacc
    56761 cgactcggta ttggtcgccc aggccatcac cacatttccg caaccagcca gttgggtaat
    56821 ttgctgccag atcatctccc gaatacgttt tgatgtatca ccaacataca caccggcacg
    56881 cacttccagt agccagattg cgagccgtcc acgtaagcgc ggcgggacat tttctgtaac
    56941 aaccacgacc atgctcatca gccgcgtcct cgatgaccgc tatcgcccaa cgtttcaggt
    57001 tcggggatgg ctggcggtaa catatccggc gcaggttgtg gcggttcaat ttcacctgcg
    57061 gcaaggactt cctcaattaa cggtattaat ttgcccgtta acttagtgct acggaaaata
    57121 tcgcgacagg ctaatctgac ttctttatca ggttctgcgg gttgcctcgc tgctatttca
    57181 aatgcctttg gcacaaccga atcaaattta atgatatcgg ctatgtcata aacaaatgaa
    57241 agcggtttgc cactatggat aaacccaata gcgggcgcat atcccgcggc taatactgcc
    57301 gcttcagata taccgtacag acatgatgta gcagcactga tgcagcgatt cacaacatcg
    57361 cctttttccc agtctttagg atcgtatttg cgaccattcc atttcacacc atattgtttc
    57421 gccagtaatg catacgtctg gcgaacgcga gatccctcaa ttccacgtaa ctgctccact
    57481 gaacggcgag ctggctgtgg ttcacgaaaa cgtaattcat acattttgcg caccaccttc
    57541 aaccgtagat cttccgttaa agccagcttt gcctggtaga gtaatttatc tgcccgcgcc
    57601 ccaccgggtt gtccggagga gtaaacgcga acgcccgctt caccgaccca gaccagcagt
    57661 gttcccaccg tggcggccag atgcaccgcc gcgtgggaaa ctctcgttcc tggttcgagc
    57721 ataatgcagg cgaccgatcc caccggaata tgcgttcgga tccctgtttt gtcgatcagc
    57781 acgaaagcgc cgtccagtac gtcgatctga ccgtactgga ggaagatcat ggaggtgcga
    57841 tcttttaatg ggatcgggct cagtggtaca aacgtcacgc ctctgctccg ggtttgatca
    57901 gcatcagacc acagccgaac gcccggcttt taccataccc ctggctcagg cgttgcacaa
    57961 ataacccagg atcggtgacc gtaagcatcc ccgtataatc cacgctactg aactggatca
    58021 gttgccggga gttttcccgc cgcagttgct gttgtcggta ggcatcaacc gaggtatcca
    58081 gcagtgtaaa accgcttctc tccccctgcg ctgccagcca gtccagcgcc gcctgttgtt
    58141 gatgcaacca gacatcactt ccttccgcct gccccctcac ctgccgtttc gcctccatca
    58201 gcagatcgtg gcgcttgccc gctttacaga ttgttggatt cgcccgcagg ttgaaacaaa
    58261 gttgttgtcc ggtacacagc tcgggcgcaa atgaccggca ttctatggtg aacgtatcac
    58321 tttccgccgg gcgttcctgc gacagtacaa aaaagcgaaa cgcgccctgg agttcttccc
    58381 gacgataaag aaattgcctt tctttgccgc cagggaagag atcccacagc cactgatgca
    58441 tcacatattc accgcgatcc accagatgca gcaactgcgc aggcgaaagc tggccggtat
    58501 gtaaggttat tcttgagagg tacatggttc ctccttgctg agccacggcc cctgattgac
    58561 ggtgcgttcc ccaaacagcc actgctgacg atttaaagga acatcccggc ggcgtaatat
    58621 tttgctcgcg accaggccgt cgtgtttccc ttcccaccag cattcatcct gaagtttcgg
    58681 gagtgagact ttcagtttat ggaaatgatc ctgatactgt tggtatgcat tacataaggc
    58741 atcacacgca ttgccttcga gcagtaacgg cgcaagaggt aatgccagcg gatgactttt
    58801 tcgccccaga taaagcggaa aaactggatg gcgtaaaccg tcctgcaact gttcgaggct
    58861 gtaaggcgca tcggctgttg ttgccaccgc caccatccac caggcatcgg tgtagtagtc
    58921 gcgccgggag ataatcgcgc tcaaaagctc agggtcgctc aactcttcgc gacggctgaa
    58981 ataacgtgct ttacgcacct cttttggcat ctggaccgtg tgataatccc gtgcccagcg
    59041 cgggttacgg ctggcgcaaa ccaccagtga atagtggcga ttaaacgcgt ttaagcgttc
    59101 ggtatcatca cgccgaatcc ctaccccggc agccagtagc cccagcaatg ctgagcgcga
    59161 aggaagttca tgggtatgac gaacttcacc tggagcatcg accccccagg acgccatcgg
    59221 cccatgaagc tgaaaaatca gatattggct cataagccgc cccttacgcg cagataaagt
    59281 ccagcacgtc cttcatgctt ccctgtttgt tcatcacgtc aaaacttgcg cattcggtct
    59341 tctgttcgta gaccgtattc atgttttcgc gaagcgttgt aatacgctgc accgccacat
    59401 ctaactgacg tgtgccatta atgggttcat agaaagccgc cgccagagaa cgcggttgat
    59461 cggtgccttt ttctgccagc gcccaggagg cataagcccg gctggcaaag ctgttttgtt
    59521 taccggttgg ggagacttta agcgcggctt ccgtaaaggc gcgtatggtc tgattagcta
    59581 acgcttcgtc accgccgagg ttttctacca gcagatcttt atcaatgcag atataggtgt
    59641 agaacagcgc agaaccgaat ccggtttcat ctacatgacc ggcaccggca tcttcagaag
    59701 cctggcgtaa atcatctacg gcggtgaaaa aatcattttc gacaatcgtt tcactgacac
    59761 caaacgcatg cgcgacctgg caggcggctt ctacattaaa ctcgggtttt ttcgccagca
    59821 tacgaccaaa catagcgata tctaccgcca tacggtcttt acgtaacaaa gcgagatctt
    59881 tctcttttgg cgcgcgcttt tcttctgcca gttgatgggc cagcgctttt acggcgtcaa
    59941 attctgccgg gctgatatgg acgagctgtt cagtttcggc gctagtgagc ggatcttttg
    60001 gttttttgtc gtttttttgt tttttgtctt ttttggcttt cccaagataa tcggctatct
    60061 ctactgccca ttcgatggct tttttatctt cgattccttt ctcaatcagg atagttgccg
    60121 cctcacgcgc aatacgcccg ctacgaacac caatatggcc cgccagcgcc tgttcaaaaa
    60181 gttcagaagt gcgccaagca cgcttcagac tttgcgagga aacgcgcaga cgcgttgctc
    60241 cacccagaat cacagttttc ggagagccgg aatcatcacg gttaagattg gcagcagggt
    60301 aagcggttaa caaatgaagc tgaataaatg ttgtcatgag atagtccttt attaattttg
    60361 ttcgttatcg gcgtcgccag cctggtaata ttccagcgcc cagcggatac ggataaattc
    60421 tgttggtcgc tgctggcggc ggtgatgatt gagcaggtca tcgctctcct ggcaccagcg
    60481 gaagaccccc tctgccagag agtcaagatt gacggaaccg tttaataatt tgaccgcacg
    60541 gcgtaactgc cgcaataaat catccggcgt ttttactgcc aacaaacgag taaaccgccc
    60601 ctttttcatt accgccgcca gttgcgcggc aaaaggctgc cgttcgtcaa tagctttaac
    60661 attggcgcta actgctgcta tcagcgctag tgcggtgata cgccactcgg gttcatcctg
    60721 ccactttatt tgtctgttct taagaaacag gcgaaatcca tccgtcagac aaacatcatt
    60781 aaccgtcgta ctacgccgca gactggcacg ctcgccgcgt ttatcctgca actcttcatg
    60841 ccatttacgc agcgcaactt tatgctcatc ttttacaata ctcattcagc agtctcctgc
    60901 tttttctccc tggcggcgtt agcattttgc ttctccgctg atgttgtaaa atatttcttg
    60961 cgtgcggtca tgacgcgttc caaatcaacg ggctcatatg gattggtaaa gacacgatcg
    61021 tcaaaatcct gacgcgcgaa taaccacatt tccttttgcc atttactgag taattcgtac
    61081 ggatcctgac cttcttcaat tttgcgcact aatctcagga atcgatgctg agttttgttc
    61141 cagaagtcaa tatccacaaa accgaaatca agctttgtgg ttttcttctc agagaaccat
    61201 gcttctttca acgctcggtg taacaagctt agaatgtgtg atgccgcttg cgctgccaga
    61261 cgcaacttag gtattaggtc ttcttttttg tttaacaaca gcgggaaatg gtgttcatac
    61321 caacaacgaa ccttcatatc ttcaaaatca aaaccaaatc cccataagcc cacccttgtc
    61381 tgctttagat tgctggcatt caaaagtttc acgacctgtg cggggagttc agtattgttt
    61441 tttgagttac ctacctctat gaggcctaac cagtcgcgcc agattaaacc accaggttgt
    61501 ggtttaactg agtaaaaatc tccaccctct ttaagcggca gacggtaagg tgttagagga
    61561 tggcgccaca tgacgtattg aacaccatag tttttgagtg acatcaagcc caaaagagca
    61621 tcgctctgcc taccgcaaat gtcgcagtta cctattgagg ttgtcttaaa atcaatacgt
    61681 atacgtcgtg gcattcccca gtaggcctga agtttattga cttgttcatc cgttaccaca
    61741 gcaccgtcca gttcgctggt acgcgtcggt gcaagccaag gaaagaccag gtcatcaaag
    61801 gtttttggta aaggtaaatc ggcttcatct tgcggcatta cgtttagcca cagctttcgc
    61861 cacaaagggg tttgttgatt gccctgatac tccagcaatt ctataagtgt cgtcagaggc
    61921 cctccgccac gtagcccagg atagtaaccg cgtccaccta taggtgcatt caactggatt
    61981 gaaaaaagcg ctaaaggcac acaatgcagg cacagatgct gagttgtgtc tcgcttaata
    62041 aaatggtctg tatttcgttc ttttgtttgt tttcctggtg cgtcaggaag aagcgaagca
    62101 atagaagttg ccttaacttt taattcatca aaatcctgca taaatgaagg ggaatctggc
    62161 ccaaactgga aagcgtgttc taaggacagc aatgcttccc gtagcttttc agcctccagt
    62221 ccgtcttgcc agatatcatc ccaacgaccg tgatttttgg gcgcgaagct ggtttgtagt
    62281 agccccagca aaaactgcca ggccgcaccc tggagatctg cccgcggcgc agcgatatcg
    62341 acaacatttt catcagccag atcgactggt gccagcttgc ctgtcgttcc gtctttaaaa
    62401 cgaacaggca accacggggt tgtcagaagt gaaaacgagt tcatacgcat cctctcgtaa
    62461 aaaagaccat cccaggtcgg ttgtccatct ttccatcctt ttcggtgtat caactattca
    62521 gagagtcctg tcgcagacat ctcaaccaat cttgccaatc aatccttccg tagccgaata
    62581 cccgcaactt tcgtcatcgt tgactaaaat cacgtttgcc atctccggat cttgtcgctg
    62641 ttcaatgcac cacagcctga acgcctctcc ctccagtaaa gaaaactcat cccgatgttt
    62701 tttccaccag cttcggcgca ctctgacaac gctcatttcc catgcgtgag caccggtggc
    62761 ataaggcttc accacaccgt caatacaggt agccagccac agggaaacag actcctcagc
    62821 cagacgtgtc gacagttttt ccgggaggta atcgttgata ttggcggcat agccgggcct
    62881 gaagttcagc acaaactttt tagccattgc gcgatcacag taatatttgc ccacttgctc
    62941 ctgctcgctg cgggcaaatc cttccggcat taccacgtcc tcaccgtaga cggattcaat
    63001 aagaagccgg gctgcgtgtg gcatctgaat agcaccttgc tcacgcagca cacgctgcgt
    63061 cagccagatt cgcccatgat cgggatagac aaatgcactg ttacgcatgg cactgccaaa
    63121 ccattcgtcg ccaggagagt cgtcccagac gggagccaga atcagcaatt caggggggga
    63181 acgctcgtcc tttccgtcac gctttaactg accattaata tcgcggatat gccgctgtaa
    63241 tcgtcctgct cgctgaatca gcaaatcaac aggggccagg tcggagatca tttcgtccag
    63301 gtcaatatcc acgctttgct caattacttg tgtacaaaca atgacttgcg acgctctctg
    63361 taaactacaa tatttaccaa agcgtgccag tgtctccgtt tcaattcgct ggcgatcgct
    63421 aaaagcaaag cggctatgaa aaagtgaaag gctggaagca ggaatgacgc cgcgggcaag
    63481 cagctgacga tgaaccttaa tagcgtcatc gacagaattc cggatccagg cgatgcattt
    63541 cccctgactt accgccgatt cgatacgcgc aatacactct tgttcactat gaagccaacc
    63601 cacgctgacg ctacgctcaa cgtctttgcg cgtcgctacc cggtgtgagt tcacatcgga
    63661 tttcgtgaca tgcgtcagcc aggggtaatc atccttttcc aggaacggag cttcttgcag
    63721 gccctctgta ccccgcgcaa aggcagcgac gagtttgtcg cgctgctgtt gagataacgt
    63781 ggcagaaagc aaaataacgc tgtttccgcc acgcgcctgc cgctcgatca acccttcaag
    63841 aatgcacgac atgtaagcgt cacaggcgtg gatctcatca gccagcagga ttttgttact
    63901 cagccccaga agccgcagat tattatgttt gaacggcatc accgccatca tcgcctgatc
    63961 cagcgtgccg acgccaattt cagccagcaa cgccttcttg ttactgtcgg caaaccaggc
    64021 cgcacatccc tgactgaacg tttgttcatc cggttcttct gacccgacta aatcaccaga
    64081 ccagagtgat tcattaaagc ggtccattaa tgtgcgggca ctatgtgcca gcaccaggct
    64141 ggggcgggat tctggcgaat agaaagcaag ccaggttttg accagccgat cgtacatggc
    64201 attggccgtt gccattgttg gcagaccaaa aaacagcccc tgagctttcc ccgcagctat
    64261 caacctgtgc gccaggataa gcgccgcttc tgttttacct gcgccagtca catcttccag
    64321 aataaataac tgcggtcctt gctggctgat atccagatcc agcacctttt gctgtaacgg
    64381 tgtcggattc tcaataaaag gaaacagcgt attaattccg ttgaaaggcg cgacttttgc
    64441 ttttagagga aagacggtta aggcgttttg agcctgaatt aaagttttct gccagtaacc
    64501 tttaatatcc attgggtgtg cgacgcgtgg aaaaaatcgc gttgacgaac ccgtccagtc
    64561 tgcgagcacg acggttgcag agatatacca ggaaaggtgt tttataagtt caatgccctc
    64621 atcatcatcc cagaatgcgg ggatctctat gagcggaaac agtggtttga tttcaaggag
    64681 aaaatctcgt gctgcagctt tgtcttcagg cagaaaatta tccaattcat ccatacgatc
    64741 agggggccga ccatgatgcc ctgtcgtcac gggcatccac atttctatca ctcgcctaag
    64801 tttgcgcgga gagagcgaag atgaaggaag taactcctga cattcactta aataatgatt
    64861 ccacaaccag tagcccagcg ttgaatgaga gattttttcg taattttttc tggcaccttc
    64921 ctgaatcttg agttcagggg gcaggtaaag ttgttgaaaa gagcgggcaa attttccaat
    64981 atcgtgccag cacagcaacc aggcgaaaaa ttgagccgcc tgttccttgt cagaaatccc
    65041 taattgacga aaataatcag ccagcccgaa gcaatttctt ttaaccatta aatagcccat
    65101 cgcggctaca tcca
//
LOCUS       DAZFQD010000012        64170 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.012, whole genome shotgun sequence.
ACCESSION   DAZFQD010000012 DAZFQD010000000
VERSION     DAZFQD010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 64170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 64170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64170
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.012"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..814)
                     /gene="rfbA"
                     /locus_tag="ACHKTW_000843"
     CDS             complement(<1..814)
                     /gene="rfbA"
                     /locus_tag="ACHKTW_000843"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001367822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFO6461913.1"
                     /translation="MKGIILAGGSGTRLYPITMGISKQLLPVYDKPMIYYPLSVLMLA
                     GIRDILIITTPEDQFSFIRLLGDGKHLGVSISYAVQPRPDGLAQAFTIGKDFIGADSV
                     CLVLGDNIFFGQGFTPKLKTACERKLGATIFGYQVRDPGRFGVVEFDSDYRVLSIEEK
                     PLKPKSNWAVTGLYFYDNDVVEIAKSITPSQRGELEITSINEVYLKNNKLTVELLGRG
                     FAWLDTGTHDSLIEAGSFVETVQKRQGMMVACPEEIAWRNGWLNNSDLKSLAT"
     gene            complement(811..1887)
                     /gene="rfbB"
                     /locus_tag="ACHKTW_000844"
     CDS             complement(811..1887)
                     /gene="rfbB"
                     /locus_tag="ACHKTW_000844"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000699441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFO6461914.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYIFEHADICDAAAMARIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALDSDKKNSFRFHHISTDEVYGDLPHPDEVNNTEELPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGDQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEKSYREQITYVADRPGHDRRYAIDAEKISRELGWKPQETFESGIRKTINWY
                     LNNNDWCRRVQDGSYQRERLGVIR"
     gene            complement(2260..3153)
                     /gene="galF"
                     /locus_tag="ACHKTW_000845"
     CDS             complement(2260..3153)
                     /gene="galF"
                     /locus_tag="ACHKTW_000845"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HFO6461915.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVTIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGANFRKGIEKLLSE"
     gene            complement(3328..4722)
                     /gene="wcaM"
                     /locus_tag="ACHKTW_000846"
     CDS             complement(3328..4722)
                     /gene="wcaM"
                     /locus_tag="ACHKTW_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HFO6461916.1"
                     /translation="MPFKKLSRRTFLTASSALAFLHTPFARALPDQQSVNINDYNPHD
                     WIASFKQAFSEGQTVVVPAGFVCDNINTGIFIPPGKTLHILGSLRGNGRGRFVLQDGS
                     QVTGEKGGSMHNITLDVRGSDCTIKGLTMSGFGPVTQIYIGGKNKRVMRNLTIDNLTV
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDRDILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIK
                     ARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNF
                     RVNNIQLDNTHLAYKLRGIQISAGNALSFVALTNIEMKRASLELHNKPQHLFMRNIKV
                     MQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNERGQSSVDIDRINHHIV
                     NVEKINFRLPERRE"
     gene            complement(4733..5953)
                     /gene="wcaL"
                     /locus_tag="ACHKTW_000847"
     CDS             complement(4733..5953)
                     /gene="wcaL"
                     /locus_tag="ACHKTW_000847"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HFO6461917.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIVALQKGDTQN
                     THAAWTKYNLAARTRWLQDEPAGKVAKLRHRASQTLRGIYRKNTWQALNLKRYGAESR
                     NLILSAICGQVATPFHADVFIAHFGPAGVTAAKLRELGVIRGKIATIFHGIDISSRDV
                     LNHYTPEYQQLFRRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKA
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGVAFRYRILGIGPWERRLRTLIEQYQ
                     LEDVVEMPGFKPSHEVKAMLDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLVPENDARALAQRLAAFSQLDTDELAPVVKRAREKVEHDFNQ
                     QVINRELASLLQAL"
     gene            complement(5950..7230)
                     /gene="wcaK"
                     /locus_tag="ACHKTW_000848"
     CDS             complement(5950..7230)
                     /gene="wcaK"
                     /locus_tag="ACHKTW_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HFO6461918.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNPQAEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMKQHNSAAGVVGRVKKVLRRRYQHQVLLSRVTDTGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
                     FQDEQFNQLANYVFGHCDALILRESVSLDLMKRSNITTAKVEHGVDTAWLVDHHTEDF
                     TASYAVQHWLDVAAQQKTVAITLRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLEMGKILGACELTVGT
                     RLHSAIISMNFATPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQ
                     LPALNARLSEAVSRERQTGMQMVQSVLERIGEVK"
     gene            complement(7409..8887)
                     /gene="wzxC"
                     /locus_tag="ACHKTW_000849"
     CDS             complement(7409..8887)
                     /gene="wzxC"
                     /locus_tag="ACHKTW_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid undecaprenyl disphosphate flippase
                     WzxC"
                     /protein_id="HFO6461919.1"
                     /translation="MSLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGLVVCVAVFLLSDAI
                     GDVLNNPDLAPLIKTLSLAFVVIPHGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYCPGLHFSLASVAPNLRFGAWLTA
                     DSIINYLNTNLSTLVLARILGAGVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRVNFYKLLSVVGIINFPALLGLMVVSNNFVPLVFGEKWISIIPVLQLLCIVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIVIGGQMAGAIGVTLGFLLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLVVSYALGIVLKGQLALGMLLA
                     VQIATGVLAFVVMIVLSRHPLVVEVKRQFCRSEKMKMLLRAG"
     gene            complement(8889..10283)
                     /gene="wcaJ"
                     /locus_tag="ACHKTW_000850"
     CDS             complement(8889..10283)
                     /gene="wcaJ"
                     /locus_tag="ACHKTW_000850"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HFO6461920.1"
                     /translation="MTNLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSF
                     LYMHLLVALITLVVFQMLGGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFN
                     NDFDTQLKIWLAWYGLTSIGLVVCRSCIRIGAGWLRNHGYNKRMVAVAGDLAAGQMLM
                     ESFRNQPWLGFEVVGVYHDPKPGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGA
                     RVKKLVHQLADTTCSVLLIPDVFTFNILHSRLEEMNGVPVVPLYDTPLSGVNRLIKRA
                     EDIVLATLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVME
                     NDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYR
                     QLIEGYMLRHKVKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIV
                     FLTVFKGFVNKAAY"
     gene            complement(10338..11708)
                     /gene="cpsG"
                     /locus_tag="ACHKTW_000851"
                     /gene_synonym="manB"
     CDS             complement(10338..11708)
                     /gene="cpsG"
                     /locus_tag="ACHKTW_000851"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HFO6461921.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     SYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTVDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAHPIEAINR
                     VEQHFSREALAVDRTDGISMTFADWRFNLRSSNTEPVVRLNVESRGDVPLMEARTRTL
                     LTLLNE"
     gene            complement(11901..13337)
                     /gene="cpsB"
                     /locus_tag="ACHKTW_000852"
     CDS             complement(11901..13337)
                     /gene="cpsB"
                     /locus_tag="ACHKTW_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HFO6461922.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPENDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSAGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMERTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            complement(13340..14563)
                     /gene="wcaI"
                     /locus_tag="ACHKTW_000853"
     CDS             complement(13340..14563)
                     /gene="wcaI"
                     /locus_tag="ACHKTW_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HFO6461923.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGGKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAADNIIFFPNWSEIARFQHVADADV
                     DALRNQLGLPDNKKIILYSGNIGEKQGLENVIEAADRLRDEPLIFAIVGQGGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKYNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            complement(14560..15039)
                     /gene="gmm"
                     /locus_tag="ACHKTW_000854"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             complement(14560..15039)
                     /gene="gmm"
                     /locus_tag="ACHKTW_000854"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HFO6461924.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEELLLPDEQHDDYRWLTPDALLASDNVHANSRAYFLAEKRAGVPGL"
     gene            complement(15039..16007)
                     /gene="fcl"
                     /locus_tag="ACHKTW_000855"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             complement(15039..16007)
                     /gene="fcl"
                     /locus_tag="ACHKTW_000855"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HFO6461925.1"
                     /translation="MSRQRIFIAGHRGMVGSAIRRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     LFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRGE"
     gene            complement(16010..17131)
                     /gene="gmd"
                     /locus_tag="ACHKTW_000856"
     CDS             complement(16010..17131)
                     /gene="gmd"
                     /locus_tag="ACHKTW_000856"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0019673 - GDP-mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HFO6461926.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            complement(17157..17705)
                     /gene="wcaF"
                     /locus_tag="ACHKTW_000857"
     CDS             complement(17157..17705)
                     /gene="wcaF"
                     /locus_tag="ACHKTW_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HFO6461927.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDEVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRERVETE"
     gene            complement(17721..18467)
                     /gene="wcaE"
                     /locus_tag="ACHKTW_000858"
     CDS             complement(17721..18467)
                     /gene="wcaE"
                     /locus_tag="ACHKTW_000858"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HFO6461928.1"
                     /translation="MLLSIITVAFRNLEGIVKTHASLAHLAQAEDISFEWIVVDGGSN
                     DGTREYLENLNGIYNLRFVSEPDNGIYDAMNKGITMAQGKFALFLNSGDIFHQNAANF
                     VRKLKMQKDNVMITGDALLDFGDGHKIKRSAKPGWYIYHSLPASHQAIFFPVSGLKKW
                     RYDLEYKVSSDYALAAKMYKAGYAFKKLNGLVSEFSMGGVSTTNNMELCADAKKVQRQ
                     ILHVPGFWAELSWHLRQRTTSKTKALYNKV"
     gene            complement(18478..19695)
                     /gene="wcaD"
                     /locus_tag="ACHKTW_000859"
     CDS             complement(18478..19695)
                     /gene="wcaD"
                     /locus_tag="ACHKTW_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid polymerase WcaD"
                     /protein_id="HFO6461929.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISVKKLMIALGIGAGLTAFNYLFGQSLDAGKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVVGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDAMILAGIILSGSFSGV
                     MTFILFYLLEWVFQYLNKEAIKKKLPLALISLAVFLVGVVIAFPYISTRLGDLGTEGS
                     SSYYRIVGPLVMVGYSLTHIDGVVRFGSLYEYVASFGIFNGADVGKTIDNGLYLLIIY
                     FSWFAVFLSLWYMGKVIKMMINAFGDNRNFRVQLYLFTPVSLFFTGSIFSPEYAFLIV
                     CPFILRKALNITR"
     gene            complement(19670..20887)
                     /gene="wcaC"
                     /locus_tag="ACHKTW_000860"
     CDS             complement(19670..20887)
                     /gene="wcaC"
                     /locus_tag="ACHKTW_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HFO6461930.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIVLFRLFNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLKSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVSEEEVLQLVQLSKPEIAQAIFGTTLAEFSQRSRAAYSGQ
                     QMLEEYVNFYQNL"
     gene            complement(20884..21372)
                     /gene="wcaB"
                     /locus_tag="ACHKTW_000861"
     CDS             complement(20884..21372)
                     /gene="wcaB"
                     /locus_tag="ACHKTW_000861"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HFO6461931.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNIWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            complement(21375..22214)
                     /gene="wcaA"
                     /locus_tag="ACHKTW_000862"
     CDS             complement(21375..22214)
                     /gene="wcaA"
                     /locus_tag="ACHKTW_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
                     /protein_id="HFO6461932.1"
                     /translation="MKDNPLISIYMPTWNRQQLAIRAIKSVLRQDYSNWEMIIVDDCS
                     TSWEQLQQYVTALNDPRITYIHNDINSGACAVRNQAIMLAQGEYITGIDDDDEWTPNR
                     LSVFLAHKQQLVTHAFLYANDYVCQGEVYSQPASLPLYPKSPYSRRLFYKRNIIGNQV
                     FTWAWRFKECLFDTELKAAQDYDIFLRMVVEYGEPWKVEEATQILHINHGEMQITSSP
                     KKFSGYFHFYRKHKDKFDRASKKYQLFTLYQIRNKRMTWRTLLTLLSVRNGKRLADGI
                     RGR"
     gene            complement(22392..24554)
                     /gene="wzc"
                     /locus_tag="ACHKTW_000863"
     CDS             complement(22392..24554)
                     /gene="wzc"
                     /locus_tag="ACHKTW_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310892.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HFO6461933.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITAVFALC
                     AVVYTFFATPIYSADALVQIEQNSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWDRLMGRQNETVKVTTFNRPKEMADQVFTLNVLDDK
                     NYTLSSDGGFSARGQAGQILKKEGVTLMVEAIHARPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEDEKAKLNGRVTAMPKTQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIVDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGIKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVNWASKNYDLVLIDTPPILAVTDAAIVGRHVGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            complement(24557..25000)
                     /gene="wzb"
                     /locus_tag="ACHKTW_000864"
     CDS             complement(24557..25000)
                     /gene="wzb"
                     /locus_tag="ACHKTW_000864"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HFO6461934.1"
                     /translation="MFNNILVVCVGNICRSPTAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMEKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYRKSRETFAAVYTLLERSARQWEQALNAEQV"
     gene            complement(25006..26145)
                     /gene="wza"
                     /locus_tag="ACHKTW_000865"
     CDS             complement(25006..26145)
                     /gene="wza"
                     /locus_tag="ACHKTW_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HFO6461935.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALGNAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            26454..26603
                     /locus_tag="ACHKTW_000866"
     CDS             26454..26603
                     /locus_tag="ACHKTW_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6461936.1"
                     /translation="MVIEYWLSNNAGGSVTANGIAGKVTIRQLAIFKNYIKCHSIWFL
                     GVSLG"
     gene            26804..28387
                     /gene="yegH"
                     /locus_tag="ACHKTW_000867"
     CDS             26804..28387
                     /gene="yegH"
                     /locus_tag="ACHKTW_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFO6461937.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAETASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEDEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLHKDGELEYEV"
     gene            complement(28661..30514)
                     /gene="asmA"
                     /locus_tag="ACHKTW_000868"
     CDS             complement(28661..30514)
                     /gene="asmA"
                     /locus_tag="ACHKTW_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HFO6461938.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQAQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGQVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILNAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(30536..31117)
                     /gene="dcd"
                     /locus_tag="ACHKTW_000869"
     CDS             complement(30536..31117)
                     /gene="dcd"
                     /locus_tag="ACHKTW_000869"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006229 - dUTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HFO6461939.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(31209..31850)
                     /gene="udk"
                     /locus_tag="ACHKTW_000870"
     CDS             complement(31209..31850)
                     /gene="udk"
                     /locus_tag="ACHKTW_000870"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HFO6461940.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            32167..35482
                     /gene="dgcE"
                     /locus_tag="ACHKTW_000871"
                     /pseudo
     CDS             32167..35482
                     /gene="dgcE"
                     /locus_tag="ACHKTW_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
     gene            complement(35521..36378)
                     /gene="alkA"
                     /locus_tag="ACHKTW_000872"
     CDS             complement(35521..36378)
                     /gene="alkA"
                     /locus_tag="ACHKTW_000872"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HFO6461941.1"
                     /translation="MYTLNWQPPYDWSWMLGFLAARAVSGVETVADDYYARSLAVGEY
                     RGVVTAIPDMARHTLHINLSAGLEPVAAECLAKMSRLLDLQCNPQIVNGALGKLGAAR
                     PGLRLPGCVDAFEQGVRAILGQLVSVAMAAKLTAKVVQLYGERLDDFPEYICFPTPQR
                     LAAADPQALKALGMPLKRAEALIHLANAALEGSLPMTIPGDVEQAMKTLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKQRFPGMTPAQIRRYAERWKPWRSYALLHIWYTEG
                     WQPDGTDEL"
     gene            36512..37864
                     /gene="yegD"
                     /locus_tag="ACHKTW_000873"
     CDS             36512..37864
                     /gene="yegD"
                     /locus_tag="ACHKTW_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFO6461942.1"
                     /translation="MFIGFDYGTANCSVAVMRDGKPQLLKMENDSTLLPSMLCAPTRE
                     AVSEWLYRHHDVPADDDETQALLRRAIRYNREEDIDVTAKSVQFGLSSLAQYIDDPEE
                     VWFVKSPKSFLGASGLKPQQVALFEDLVCAMMLHIRQQAQAQLPEAITQAVIGRPINF
                     QGLGGDEANAQAQGILERAAKRAGFRDVVFQYEPVAAGLDYEATLQEEKRVLVVDIGG
                     GTTDCSLLLMGPQWRSRLDREASLLGHSGCRIGGNDLDIALAFKNLMPLLGMGGETEK
                     GIALPILPWWNAVAINDVPAQSDFYSSANGRLLNDLVRDAREPEKVALLQKVWRQRLS
                     YRLVRSAEECKIALSSVAETRASLPFISNELATLISQRGLESALSQPLARILEQVQLA
                     LDNAQEKPDVIYLTGGSARSPLIKKALAEQLPGIPIAGGDDFGSVTAGLARWAEVVFR
                     "
     gene            complement(37877..39817)
                     /gene="yegI"
                     /locus_tag="ACHKTW_000874"
     CDS             complement(37877..39817)
                     /gene="yegI"
                     /locus_tag="ACHKTW_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YegI"
                     /protein_id="HFO6461943.1"
                     /translation="MKPTLYTATGECVTPGRELGKGGEGAVYDINEFVDSVAKIYHTP
                     PPALKQDKLAFMAATADAQLLNYVAWPQATLHGGRGGKVIGFMMPKVSGKEPIHMIYS
                     PAHRRQRYPHCAWDFLLYVARNIASSFATVHEHGHVVGDVNQNSFMVGRDSKVVLIDS
                     DSFQINANGTLHLCEVGVSHFTPPELQTLSSFVGFERTENHDNFGLALLIFHVLFGGR
                     HPYSGVPLISDAGNALETDITHFRYAYASDNQRRGLKPPPRSIPLSMLPSDVEAMFQQ
                     AFTESGVATGRPTAKAWVAALDSLRQQLKKCTVSAMHVYPGHLADCPWCALDNQGVIY
                     FIDLGEEVITTGGDFVLAKVWAMVMASVAPPALQLPLPDHFQPTGRPLPLGLLRREYI
                     ILLEIALSALSLLLCGLQAEPRYIILVPVLAAIWIIGSLTSKAYKAEVQQRREAFNRA
                     KMDYDHLVRQIQQVGGLEGFIAKRTMLEKMKDEILGLPEEEKRALAALHDTARERQKQ
                     KFLEGFFIDVASIPGVGPARKAALRSFGIETAADVTRRGVKQVKGFGDHLTQAVIDWK
                     ASCERRFVFRPNEAITPADRQAVMAKMTAKRHRLESALTVGATELQRFRLHAPARTMP
                     LMEQLRQAAEKLAQAQADLSRC"
     gene            complement(39814..40575)
                     /gene="pphC"
                     /locus_tag="ACHKTW_000875"
     CDS             complement(39814..40575)
                     /gene="pphC"
                     /locus_tag="ACHKTW_000875"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family serine/threonine-protein
                     phosphatase"
                     /protein_id="HFO6461944.1"
                     /translation="MSWRLVYASAVGTSHISADLPCQDACQMQVAWLNDQQPLLVMFL
                     ADGAGSVSQGGEGAMLAVNEAMAYMSQKVQGGELGLNDILATDIVLTVRQRLFAEAEA
                     KELAVRDFACTFLGLISSANGTLIMQIGDGGVVVDFGHGLQLPLTPMVGEYANMTHFI
                     TDEDAVSRLETFTSTERVHKVAAFTDGIQRLALNMLDNSPHVPFFTPFFNGLASATQE
                     QLDLLPELLKQFLSSPAVNERTDDDKTLALALWLP"
     gene            complement(40572..41231)
                     /gene="yegL"
                     /locus_tag="ACHKTW_000876"
     CDS             complement(40572..41231)
                     /gene="yegL"
                     /locus_tag="ACHKTW_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HFO6461945.1"
                     /translation="MSEQITFATSDFASNPEPRCPCILLLDVSGSMSGRPINELNAGL
                     VTFRDELLADSLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGAAITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPTDEWQAAANKVFQGEEDKKFAFFSIA
                     VQGADMKTLAQISVRQPLPLQGLQFRELFSWLSSSLRSVSRSTPGTEVVLEAPKGWTS
                     V"
     gene            complement(41452..41511)
                     /gene="ibsA"
                     /locus_tag="ACHKTW_000877"
     CDS             complement(41452..41511)
                     /gene="ibsA"
                     /locus_tag="ACHKTW_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsA"
                     /protein_id="HFO6461946.1"
                     /translation="MMKHVIILVILLVISFQAY"
     gene            complement(41784..41840)
                     /locus_tag="ACHKTW_000878"
     CDS             complement(41784..41840)
                     /locus_tag="ACHKTW_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFO6461947.1"
                     /translation="MMKLLIIVVLLVISFPAY"
     gene            42119..43366
                     /gene="mdtA"
                     /locus_tag="ACHKTW_000879"
     CDS             42119..43366
                     /gene="mdtA"
                     /locus_tag="ACHKTW_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
                     /protein_id="HFO6461948.1"
                     /translation="MKGSYKSRWVIVIVVVIAAIAAFWFWQGRNDSQSAAPGATKQAQ
                     QSPAGGRRGMRSGPLAPVQAATAVEQAVPRYLTGLGTIIAANTVTVRSRVDGQLIALH
                     FQEGQQVKAGDLLAEIDPSQFKVALAQAQGQLAKDKATLANARRDLARYQQLAKTNLV
                     SRQELDAQQALVSETEGTIKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQIS
                     SGDTTGIVVITQTHPIDLVFTLPESDIATVVQAQKVGKPLVVEAWDRTNSKKLSEGTL
                     LSLDNQIDATTGTIKVKARFNNQDDALFPNQFVNARMLVDTEQNAVVIPTAALQMGNE
                     GHFVWVLNSENKVSKHLVTPGIQDSQKVVIRAGISAGDRVVTDGIDRLTEGAKVEVVE
                     AQSATTPEEKATSREYAKKGARS"
     gene            43366..46488
                     /gene="mdtB"
                     /locus_tag="ACHKTW_000880"
     CDS             43366..46488
                     /gene="mdtB"
                     /locus_tag="ACHKTW_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
                     /protein_id="HFO6461949.1"
                     /translation="MQVLPPSSTGGPSRLFIMRPVATTLLMVAILLAGIIGYRALPVS
                     ALPEVDYPTIQVVTLYPGASPDVMTSAVTAPLERQFGQMSGLKQMSSQSSGGASVITL
                     QFQLTLPLDVAEQEVQAAINAATNLLPSDLPNPPVYSKVNPADPPIMTLAVTSTAMPM
                     TQVEDMVETRVAQKISQISGVGLVTLSGGQRPAVRVKLNAQAIAALGLTSETVRTAIT
                     GANVNSAKGSLDGPSRAVTLSANDQMQSAEEYRQLIIAYQNGAPIRLGDVATVEQGAE
                     NSWLGAWANKEQAIVMNVQRQPGANIISTADSIRQMLPQLTESLPKSVKVTVLSDRTT
                     NIRASVDDTQFELMMAIALVVMIIYLFLRNIPATIIPGVAVPLSLIGTFAVMVFLDFS
                     INNLTLMALTIATGFVVDDAIVVIENISRYIEKGEKPLAAALKGAGEIGFTIISLTFS
                     LIAVLIPLLFMGDIVGRLFREFAITLAVAILISAVVSLTLTPMMCARMLSQESLRKQN
                     RFSRASEKMFDRIIAAYGRGLAKVLNHPWLTLSVALSTLLLSVLLWVFIPKGFFPVQD
                     NGIIQGTLQAPQSSSFANMAQRQRQVADVILQDPAVQSLTSFVGVDGTNPSLNSAHLQ
                     INLKPLDERDDRVQKVIARLQTAVDKVPGVDLFLQPTQDLTIDTQVSRTQYQFTLQAT
                     SLDALSTWVPQLMEKLQQLPQLSDVSSDWQDKGLVAYVNVDRDSASRLGISMADVDNA
                     LYNAFGQRLISTIYTQANQYRVVLEHNTENTPGLAALDTIRLTSSDGGVVPLSSIAKI
                     EQRFAPLSINHLDQFPVTTISFNVPDNYSLGDAVQAIMDTEKTLNLPVDITTQFQGST
                     LAFQSALGSTVWLIVAAVVAMYIVLGILYESFIHPITILSTLPTAGVGALLALMIAGS
                     ELDVIAIIGIILLIGIVKKNAIMMIDFALAAEREQGMSPRDAIYQACLLRFRPILMTT
                     LAALLGALPLMLSTGVGAELRRPLGIGMVGGLIVSQVLTLFTTPVIYLLFDRLALWTK
                     SRFARHEEEA"
     gene            46489..49566
                     /gene="mdtC"
                     /locus_tag="ACHKTW_000881"
     CDS             46489..49566
                     /gene="mdtC"
                     /locus_tag="ACHKTW_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HFO6461950.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLNPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVAVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNIWLYISIPKTFFPEQDTGVLMGGIQA
                     DQSISFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQ
                     QIIDRLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRK
                     KLATLPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIY
                     QPMNQYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGL
                     SAASTISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILI
                     IAAIATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLI
                     GIVKKNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSG
                     GDGSELRQPLGITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            49567..50982
                     /gene="mdtD"
                     /locus_tag="ACHKTW_000882"
     CDS             49567..50982
                     /gene="mdtD"
                     /locus_tag="ACHKTW_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO6461951.1"
                     /translation="MTDLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLMLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLTIAGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNCFGYRRVLVATT
                     LGLSLVTLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHVSVDSGTTQTVFMYTWLSMALIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            50979..52382
                     /gene="baeS"
                     /locus_tag="ACHKTW_000883"
     CDS             50979..52382
                     /gene="baeS"
                     /locus_tag="ACHKTW_000883"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HFO6461952.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQMLGDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLQISAGQRDKTVRLTFAD
                     SAPGVSDDQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            52379..53101
                     /gene="baeR"
                     /locus_tag="ACHKTW_000884"
     CDS             52379..53101
                     /gene="baeR"
                     /locus_tag="ACHKTW_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HFO6461953.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLPYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDIPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREHLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            53292..53624
                     /gene="yegP"
                     /locus_tag="ACHKTW_000885"
     CDS             53292..53624
                     /gene="yegP"
                     /locus_tag="ACHKTW_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HFO6461954.1"
                     /translation="MAGWFELIKSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKVNGT
                     SQTVKDNT"
     gene            53833..54129
                     /locus_tag="ACHKTW_000886"
     CDS             53833..54129
                     /locus_tag="ACHKTW_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001307279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051983 - regulation of chromosome
                     segregation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HFO6461955.1"
                     /translation="MTYTDVRFLLTGGRKMARTMTVDLGDELREFIESLIESGDYRTQ
                     SEVIRESLRLLREKQAESRLQALRDMLAEGLSSGEAQPWEKDAFLRKVKAGIRK"
     gene            54131..54427
                     /locus_tag="ACHKTW_000887"
     CDS             54131..54427
                     /locus_tag="ACHKTW_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO6461956.1"
                     /translation="MRIIKLMPKANEDLEGIWYYSYHHFGEPQADRYVEHLSDVLQIL
                     SNNNIGTPRPELGEGIFVLPFERHVIYFLQSPGEIIVIRILNQNQDATRHLHWS"
     gene            54530..55891
                     /gene="yegQ"
                     /locus_tag="ACHKTW_000888"
     CDS             54530..55891
                     /gene="yegQ"
                     /locus_tag="ACHKTW_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HFO6461957.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIRNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPIAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(56221..56538)
                     /gene="yegR"
                     /locus_tag="ACHKTW_000889"
     CDS             complement(56221..56538)
                     /gene="yegR"
                     /locus_tag="ACHKTW_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YegR"
                     /protein_id="HFO6461958.1"
                     /translation="MKKIAAISLISIFIVSGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDAAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CGE"
     gene            56944..57843
                     /gene="yegS"
                     /locus_tag="ACHKTW_000890"
     CDS             56944..57843
                     /gene="yegS"
                     /locus_tag="ACHKTW_000890"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HFO6461959.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARYVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDDIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
     gene            complement(57925..58698)
                     /locus_tag="ACHKTW_000891"
     CDS             complement(57925..58698)
                     /locus_tag="ACHKTW_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFO6461960.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSISGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIITDDAVSEVDKLALQKTRVKLITV"
     gene            complement(58804..59844)
                     /gene="gatD"
                     /locus_tag="ACHKTW_000892"
     CDS             complement(58804..59844)
                     /gene="gatD"
                     /locus_tag="ACHKTW_000892"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFO6461961.1"
                     /translation="MKSVVNDTDGIVRVAESVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTCPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSSEMSAP
                     QMQSVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFTQAVRDIA
                     RNAMPGKVLLIP"
     gene            complement(59892..61247)
                     /locus_tag="ACHKTW_000893"
     CDS             complement(59892..61247)
                     /locus_tag="ACHKTW_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015577 - galactitol transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015796 - galactitol transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HFO6461962.1"
                     /translation="MFSEVMRYILDLGPTVMLPVVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     GDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRVIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQLAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSPQNIPGFIIIGAIYLTGIFMTWRRARGFIKQEKVVLA
                     E"
     gene            complement(61251..61535)
                     /gene="gatB"
                     /locus_tag="ACHKTW_000894"
     CDS             complement(61251..61535)
                     /gene="gatB"
                     /locus_tag="ACHKTW_000894"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HFO6461963.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQNHNIPVELIQCRVNEI
                     ETYMDGVHLICTTAKVDRSFGDIPLVHGMPFISGVGIEALQNKILTILQG"
     gene            complement(61566..62018)
                     /gene="gatA"
                     /locus_tag="ACHKTW_000895"
     CDS             complement(61566..62018)
                     /gene="gatA"
                     /locus_tag="ACHKTW_000895"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HFO6461964.1"
                     /translation="MTNLFVRSGISFVDRSEVLTHIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVEVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE"
     gene            complement(62028..63290)
                     /gene="gatZ"
                     /locus_tag="ACHKTW_000896"
     CDS             complement(62028..63290)
                     /gene="gatZ"
                     /locus_tag="ACHKTW_000896"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
                     /protein_id="HFO6461965.1"
                     /translation="MKTLIARHKAGEHIGICSVCSAHPLVIEAALAFDRNSTRKVLIE
                     ATSNQVNQFGGYTGMTPADFREFVFTIADKVGFARERIILGGDHLGPNCWQQENADAA
                     MEKSVELVKAYVRAGFSKIHLDASMSCAGDPIPLAPETVAERAAVLCFAAESVATDCQ
                     REQLSYVIGTEVPVPGGEASAIQSVHITHVEDAANTLRTHQKAFIARGLTEALTRVIA
                     IVVQPGVEFDHSNIIHYQPQEAQALAQWIENTRMVYEAHSTDYQTRTAYWELVRDHFA
                     ILKVGPALTFALREAIFALAQIEQELIAPENRSGCLAVIEEVMLDEPQYWKKYYRTGF
                     NDSLLDIRYSLSDRIRYYWPHSRIKNSVETMMVNLEGVDTPLGMISQYLPKQFERIQS
                     GELSAIPHQLIMDKIYDVLRAYRYGCAE"
     gene            complement(63319..>64170)
                     /gene="gatY"
                     /locus_tag="ACHKTW_000897"
     CDS             complement(63319..>64170)
                     /gene="gatY"
                     /locus_tag="ACHKTW_000897"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HFO6461966.1"
                     /translation="YVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAPV
                     IIAGTPGTFTHAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVMI
                     DASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEYDVQVNEADAFYTNPAQAR
                     EFAEATGIDSLAVAIGTAHGMYASAPALDFSRLENIRQWVNLPLVLHGASGLSTKDIQ
                     QTIKLGICKINVATELKNAFSQALKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIAD
                     CGCEGRA"
BASE COUNT        15285 a        17085 c        16077 g        15723 t
ORIGIN      
        1 tagttgctaa tgatttcaaa tcagaattat ttaaccaacc atttcgccaa gcaatttctt
       61 caggacacgc taccatcata ccctgtcttt tttgtacagt ttcaacgaaa cttcccgcct
      121 caattaggct atcgtgcgtg cctgtatcta accatgcgaa accacgccca agcagttcga
      181 ccgtcaactt gttgtttttt agataaactt catttatcga agttatttct agctcacctc
      241 gttgagaagg agtaattgat ttggcaattt caacaacatc attatcataa aaataaagtc
      301 cagttacagc ccagtttgat tttggtttta acggtttttc ttcaatactt aataccctat
      361 aatctgagtc aaactctaca actccaaacc gtcctggatc tcttacctga tagccaaaaa
      421 ttgtggctcc taatttcctt tcacatgcag tttttaattt tggtgtaaac ccttgaccaa
      481 aaaaaatatt atcacctaat acaaggcaaa ctgaatcagc acctataaaa tcttttccaa
      541 ttgtgaaagc ttgcgctaaa ccatctggcc ttggttgcac tgcataacta atgctgacac
      601 ctagatgttt tccgtcacca agcaaccgta taaaactaaa ttggtcttcg ggcgttgtaa
      661 taataagtat atctctgatc cctgccaaca tcagaacaga caatggataa tatatcattg
      721 gcttgtcata aacaggcaat aattgctttg aaatccccat tgtaattgga taaagccttg
      781 ttccagaacc accagctaaa ataattcctt tcatctaatt acccccaaac gttcacgttg
      841 ataagagcca tcttgaactc gccggcacca atcattatta ttcaaatacc agttaattgt
      901 tttacgaatc ccgctctcaa acgtttcctg tggtttccat cccaattcgc ggctaatctt
      961 ctcagcatca atcgcataac ggcgatcgtg tcccggacga tcggcaacat aagtgatttg
     1021 ctcacgataa gatttctctt tcggtacaat ctcatccaac aaatcacaaa tagtgagcac
     1081 tacatcgatg tttttctttt cgttgtgtcc accaatgtta taagtttcac ccgctttacc
     1141 ttcggttacg acggtatata acgcacgtgc atgatcttca acatacagcc agtcgcgaat
     1201 ttgatcccct ttgccataaa taggtaatgc cttaccttcc agagcattaa gaattactag
     1261 tggaatcaat ttttccggaa agtgataagg accgtagtta ttcgaacaat tagtcacaat
     1321 ggtcggtaaa ccataggtac gtttccacgc acggactaaa tgatcgctgg atgctttgga
     1381 tgcggaataa gggctgcttg gcgcgtaagc tgttgtctca gtaaataagg gtaattcttc
     1441 tgtattattt acctcgtcag gatgaggcaa atcaccatat acttcgtcag tagaaatatg
     1501 atgaaaacgg aagctatttt tcttgtcgct atcaagagca gaccagtaat tgcgagcggc
     1561 ttccaaaagg acataagtac caacaatatt ggtttcaata aatgccgcag ggcctgtaat
     1621 tgaacgatcc acatggcttt cagcagccag gtgcatcact gcatccggct gatgctgagc
     1681 aaaaatccgt gccattgcag cagcatcgca aatatccgca tgttcaaaaa tatagcgttc
     1741 agaatcagaa acatcagcaa gtgattccag gtttccggcg tacgttaatt tatcgacatt
     1801 aacaacacta tcctgcgtat tatttataat gtgacgaact acagcagaac caataaatcc
     1861 tgcgccacca gtaacaagta ttttcactta atttattcca tattacatca gagcatgctg
     1921 tgaaataagc ggctctttgt ttgattaata gaggtattaa tgcacgctac cgcccctggc
     1981 tttacagcta ccagagcact gcatgcatgt ctacgatgtg acgagcgtta cccactcgcg
     2041 ctaaacccga aaaattcaaa cgctaattgt cttaccaatc cgctctggaa acaaggaaaa
     2101 tcctggaaaa ctttgactaa aatcctattg ctaactcgtt gttattctga ttgtttatat
     2161 aaaacaacgg caggaatatt cgcagcaaat tactttcgcc acgaatcttc actgccgtta
     2221 taattttctt atcaaccgtt acatccggtc agattttcat tattcgctta acagcttctc
     2281 aataccttta cggaaattcg ccccttcttt caggttgcgt agtccatact tcacaaatgc
     2341 ctgcatataa cccatttttt taccgcagtc gtagctgtct ccagtcatca gcatggcatc
     2401 aacggactgt tttttcgcca gttcagcgat ggcatcagtc agctgaatac gtccccatgc
     2461 accaggctga gtgcgttcaa gttccggcca aatatcggca gaaagcacat aacggccaac
     2521 ggccatgatg tctgagtcca gcgtctgcgg ctgatccggt ttttcgataa attcgacgat
     2581 gcggcttact ttaccttcgc ggtccagcgg ctctttggtc tgaatgacgg agtattcaga
     2641 gaggtcaccc ggcatacgtt ttgccagcac ctggctgcgt cctgtttcat tgaagcgcgc
     2701 aatcatggca gcaaggttgt agcgcagcgg gtcggcgctg gcgtcgtcga tcacaacgtc
     2761 tggcagcacc acgacaaatg ggttgtcacc aatggcgggt cgtgcacata aaatggagtg
     2821 gcccaaacct aaaggttcgc cctgacgcac gttcataatg gtcacacccg gcggacagat
     2881 ggactgcact tccgccagca gctgacgctt cacgcgctgt tcaaggagag attctaattc
     2941 ataagaggtg tcgaagtggt tttcgaccgc gttcttggac gcgtgagtta ccaggaggat
     3001 ttctttgatc cctgcagcca caatctcgtc aacaatgtac tgaatcattg gcttgtcgac
     3061 gatcggtagc atctctttgg gaatcgcctt agtggcaggc aacatatgca tcccaagacc
     3121 cgctacagga ataactgctt ttaaattcgt cattatttca tccacctgta aaatggttgc
     3181 tgaattatag cttgttcgat ttttttcgcc agcatcaatt accctgaatt gattactgaa
     3241 ttacttgtga tgttacgccg cttcgttgtg gattgcagta gcattgttcc taagtatggc
     3301 tccatttttc caggaatggt cgcaaatcta ctccctccgt tctggcaatc taaagttaat
     3361 cttttccaca ttaacaatat ggtgattaat cctgtcgata tcgacggagc tttgccctct
     3421 ttcattcacc gcatgaacat tcgcaagaga tagcagtgtt tcttttttcg ccataaagac
     3481 gccgcgaacg tctttgcgca tgtcaaagtt catgctcaat gctggtccaa ctgaggattc
     3541 ctgcatcacc ttgatatttc gcataaaaag atgttgcggt ttgttgtgta actccagcga
     3601 ggcacgcttc atctcaatgt tagtcagcgc cacaaaggag agagcattcc cggcggagat
     3661 ttgaatgccg cgcaatttgt aagcaaggtg ggtgttatcc agttgaatat tattcactcg
     3721 gaaattttgc ggtatcgaga gatatttgcc tttaattacc ccatagccga ttaacatccc
     3781 ggcgctatta atcatttcaa tattatcaat cacgaaattg tcacaaccgt aaatagcaac
     3841 tgtcgcgtta tcaatgcctg ctttcttact gaaatccggc gtgatattgc gggctttgat
     3901 attacgaata acaaaatgtt taccattttc aacatggatc aactgccgac aatccgatcc
     3961 cgtgatattc gccacgacaa agtttttcac tgcctggtct tccgggtagt tgttatcgta
     4021 ggtacttccc gcaaggccta tgccaatgcc ccagttgatt ttgccattag tacagttgat
     4081 gcgctcgatg acatggtcgg agatcaaaat atcacggtcg ttaatcgcca cgttccattc
     4141 aatggcatcg ccctgtaagt cgctgaactt acaattggtg atgttggcac cgattatctg
     4201 gttatgaaat ccctggcgta agatggcgta attagcgtgg ctaacggtca ggttatcgat
     4261 ggtcaggtta cgcatgaccc gtttgttttt gccgccgata tagatctgcg tcaccgggcc
     4321 aaagccgctc atcgtcagcc ctttgatggt gcagtcagaa ccacgcacat ccagggtgat
     4381 gttatgcata ctgccgccct tctcccctgt cacctggctg ccgtcctgta agacaaatcg
     4441 ccctctgccg ttgcctcgca ggcttccgag gatgtgtaac gttttgccag gaggaatgaa
     4501 gatgccggtg ttgatattgt cacaaacgaa tccggcaggc acgacgaccg tttgcccttc
     4561 gctgaaggct tgtttaaatg aggcgatcca gtcgtgtggg ttgtagtcgt taatgttaac
     4621 gctctgttgg tcgggaagtg cgcgagcgaa aggggtatgg aggaaggcaa gcgccgagct
     4681 tgccgtcagg aacgtgcgtc gggagagttt tttaaatggc atgcgttctc ctctataaag
     4741 cctgcagcaa gctggcgagt tctcgattga tcacctgctg gttaaaatcg tgttcgactt
     4801 tttcgcgcgc acgtttgaca accggagcca gttcgtcagt gtccagttgg ctaaatgccg
     4861 ccagacgttg tgccagtgcg cgagcatcgt tctcaggcac cagccagccg gatttatcgg
     4921 cctccaccag ttccggtatt ccgctatgca gagtagaaac tactggaatg ccgaccgcca
     4981 tcgcttccat tagcgccact ggaatgcctt ccatatcacc atccgcgccc gttaccgatg
     5041 gcaacaggaa gacatccgcg tcgtcgagca tcgctttcac ttcatggctc ggtttaaagc
     5101 ccggcatctc taccacatct tccagttgat attgttcgat gagggtgcgc aggcgtcgtt
     5161 cccacgggcc aatgccgaga atgcgatagc gaaatgccac gccctgctct ttcaactgac
     5221 ggcaggcttc aatcgccaca tgcagacctt ttttctcggt taagcgtgcg acggaaatga
     5281 tttccagcgg cgttgcgggt gctttcaccg gacgcgggct aaagcgcgtc atgtccacgc
     5341 ccatacgcga tacggcgatt ttttccctcg gacagcccat tttttgcagc cttccggccc
     5401 acagatcgct tatcggtaac atcaggtcgc cacggcgaaa cagttgttga tattcgggag
     5461 tgtagtggtt gagaacgtcc cgactggaga tatcaatacc gtggaagata gtggcaattt
     5521 tgccgcgaat aacacccagt tcgcgtagtt ttgctgcggt tacccccgcc gggccaaaat
     5581 gagcgataaa gacatcggca tgaaacggtg ttgcgacctg gccacaaatg gcagacaaaa
     5641 tcaggttccg cgactcggca ccatagcgtt tgaggttgag cgcctgccag gtatttttac
     5701 gataaatgcc gcgtaaggtc tggctggcgc ggtggcgcaa tttcgctact ttgcccgcag
     5761 gttcgtcctg taaccaacgg gttctggcgg caaggttgta tttcgtccat gccgcgtggg
     5821 tgttttgtgt gtcgcctttt tgcagcgcga caatctccac ctcaaatccc atatcaataa
     5881 acgcagtgat ttggttgagg acgaaggttt ccgacgacag cgggaatttc agtaaaaaga
     5941 agccgacctt catttcacct ccccgatgcg ctcaagcaca gactgtacca tctgcattcc
     6001 tgtctgacgc tcacgactga cggcttcgct aagtcgtgcg ttcagcgccg gaagctggcc
     6061 taaggtatcc gcaaccatcg cttgcaggct gccgtctaat aaatgacgga tatcaattgc
     6121 catctctggc agtcccaact gttgcataat cccggcggat ttatgttcgt agttgatggc
     6181 aattgccgga gtggcaaaat tcatcgagat aatggcagag tgcaggcgcg taccgacggt
     6241 gagttcacag gcaccgagaa ttttgcccat ttccagatcg ttaagttcat ccatcactac
     6301 gtgataacgg gcaggatcac tgatgtgctg gcgcaggttg agcgccacca tgcggtcgtc
     6361 tttgttatag ctgtcgatac ccgtacaggt ggagagcgca ataacctgat agccttcgtc
     6421 gagaatgcga ttgaccaccc cggcaaaggc tttttcatac gcttgttgag tggtgccgag
     6481 acgtttgtcg aacggtgcca gttcgcgcag ggtaatggcc accgttttct gttgtgcggc
     6541 aacgtccagc cagtgttgaa cggcatagct ggcggtgaaa tcttcggtgt ggtgatcgac
     6601 cagccacgcg gtatcgacgc catgttccac ttttgcggtg gtgatattgc tgcgtttcat
     6661 cagatcaagg ctgaccgatt cgcgcaggat cagcgcgtcg cagtgaccaa aaacgtagtt
     6721 cgccagttgg ttaaattgct catcctggaa cgggccaaca ctgtgaccaa tcataaacag
     6781 cggctttttc gccatgaaag tacaaagtgc atgttcaaac tgcggcacgc catagagatc
     6841 gacaaaaaac gatccgccga cctgaataat ggcgtcgtaa cctgacagca ggcgcacgaa
     6901 gtcggtgaat ccctgggcga tggcgatatt acgcagcttg ccagtgtcgg tgacgcgtga
     6961 gagcaatacc tggtgttgat agcggcgacg gaggactttt ttaacgcgcc cgacaacgcc
     7021 cgccgcgctg ttgtgttgtt tcatttgcag gaacagcgga tcgcccatta ccgggcggtt
     7081 gagcagccag gaagaactga ccggatagcg gctcatcacg tccacttcgg cttgtggatt
     7141 gaggatgttg atggcatcaa gtaagccgcg caggatggcg ctgtcgccac gattgccgca
     7201 agtgtggttg cccagaataa gtaatttcat tttttcctca taaatttgat gccaggtgag
     7261 gctgcgtttg ttttgttcgt tgccgctttt tgcctgatgc gacgctgacg cgtcttatca
     7321 ggcctacaag acccgagcct agaaccgtag gccggataag gcgtttacgc cgcatccggc
     7381 aaccgttgcc ggaaccgaaa acaacaactc accccgcccg taaaagcatt ttcatttttt
     7441 cgctgcgaca aaactgacgc tttacttcca ccaccagcgg atggcgcgac agcacaatca
     7501 tcaccacaaa cgccagcaca cccgtggcta tttgcaccgc cagcagcatc cccagcgcca
     7561 gttgcccttt cagcacaatg cccagcgcat aactgaccac cagcgtcggc agcgagagat
     7621 aaaacggcag ccataaactc aggatgtact ggcgataact tgaaccgagt actggtttaa
     7681 tcatcacgaa ataactcaga atggtgttga taatctgcac cagcaggaaa ccgagcgtga
     7741 cgccgatcgc gcccgccatc tgcccaccaa taacaatcgc cggaataaac agaaatgttt
     7801 tgaatacgtt gaatttaaag ctgatatcga cccgcgcttt cgccatcagc agcgaaccaa
     7861 tcgggttacc cactgagcgc agcagaccca caatacacag caattgcagc accggaataa
     7921 tgctgatcca cttctcacca aagaccagcg gtacaaaatt attcgacacc accatcagcc
     7981 cgagcagcgc cggaaagttg ataatcccca ccaccgacag cagcttgtag aagttgacgc
     8041 gcagcttttc ggtgtcgtcc tgaattttgg caaacgccgg aaacaacacg cgggtgatga
     8101 tggggttcag cttcatcggc ggcacaacgg ccacgttgta cgccaggttg tatccccctg
     8161 ctacgcccgc gccgagaata cgcgccagca ccagcgttga aaggttggtg ttgagatagt
     8221 tgatgatgct gtccgccgtc agccaggcac caaaacgtaa gttcggtgcc acagacgcca
     8281 gcgagaagtg cagaccgggg caataaattt tgcggccaaa gtagccaaac agcagcgttc
     8341 ttaccgcact attgaccaga taaccgagga tcgcggtcat cgccagcggc caaaaatggg
     8401 cgctaaccac tgtaaaagtg aagcccgcca gcaccgcgct ggtttcgatc atgccgattt
     8461 tgttgaactc cagttccttt tgcatcaacg cgcggaactg ctgcccgtgg gggatcacca
     8521 caaacgccag cgacaatgtt ttaatcaacg gtgccaggtc cgggttattc agcacgtcac
     8581 caatggcatc actcaacaaa aacaccgcca cgcacaccac aagccccagc ccgacgttca
     8641 gccagtacag cgtggtgagt tcaaggtggc tgatttcttt tcgctgaata atcgagttag
     8701 cgataccgaa gtcagaaagc gtatctgcca gcgcgatgat caccagcgac acggtaagca
     8761 aaccgaactg gtggttgtca ataatccgcg ccagcacggt catctgtacc agcccgaggc
     8821 cgatgatgat caccgtagca atcgccgacc acttcgcgcc gctgatggtt ttttcacgta
     8881 agctcatatc aatatgccgc tttgttaacg aaacctttga ataccgtcag gaaaacgatt
     8941 ttaatatcga accagacgct ccattcgcgg atgtactcaa ggtcgaactc gacgcgtttt
     9001 tccattttct ccagcgtgtc ggtttcgcca cgccagccat taatctgcgc ccagccggta
     9061 atgcccggtt tcactttatg gcgcagcatg tagccttcaa tgagctgtcg atactgttcg
     9121 ttatgcgcca ccgcgtgcgg acgtggaccg acaatcgaca tcccgccggt cagcacattg
     9181 ataaactgcg gcagttcatc cagcgaggtg cggcgcagaa agttccccac tttggtgacg
     9241 cgcggatcgt tctgcgtcgc ctgggtcacc actttgtcgt tctccatcac tttcatcgaa
     9301 cggaacttcc acactttgat cggcttgcca tccatgccat agcgagtctg gcggaaaata
     9361 accggccctg gtgaactgag tttcaccgcc agcgcaatac agcacagcac cggggagatc
     9421 agcagcaaga tcagcgtcgc cagcacaatg tcttccgcac gttttatcag gcggttaacc
     9481 ccggaaagcg gcgtatcgta cagcggcacc accggaacgc cgttcatctc ttcgagacgt
     9541 gaatggagaa tgttgaaggt aaaaacatcg gggatcagca gcaccgaaca ggtggtgtcc
     9601 gccaattgat ggaccagttt tttcactcgc gcgccgtcgc acatttgcat cgcgatatag
     9661 acgttatgaa tcttacccgc tttagcatcc tcgaccagct gttgcaggtt acccgcccag
     9721 tcgttagaaa cgccgcccgg tttcgggtcg tggtaaacgc ccaccacttc aaaccctaac
     9781 cacggctgat tacggaagct ctccatcagc atttgtccgg cggctaaatc ccccgccacc
     9841 gcgaccatgc gcttgttata gccatgatta cgcagccagc ccgccccaat gcgaatacat
     9901 gaacggcaaa ccaccagtcc gatactggtc agcccatacc acgccagcca gattttcagt
     9961 tgcgtgtcga aatcattgtt gaacgccacc agtccggcac tgaaaatcac gcttaaggtc
    10021 cagttttgta gcaatagggc aaattctgtc gctgcccgaa caccgcgcca tgagcgataa
    10081 aaatcggtga tgccacccag catctggaac accaccagcg taatcagcgc caccaacagg
    10141 tgcatgtaga ggaatgacag tccgctgact tcgcaaacca gccatagccc ggcaaacatg
    10201 atggtgatat ctgaaaagcg ttgcaccata gagattaacg atgcattggt tttcgctcgc
    10261 tcgcgctttt ttagatttgt catcgttgtt cctgttatta gccccttacc cgcagtgggg
    10321 taagggaaga tccgacatta ctcattcagc aacgtcagca gagttcgcgt tcgcgcttcc
    10381 atcagcggca catcaccgcg cgattccaca ttcaaccgca ccaccggttc ggtgttggag
    10441 gagcgcaggt taaagcgcca gtcggcaaag gtcatgctga tgccatcggt gcgatccacc
    10501 gccagcgcct cacggctaaa atgctgttcc acgcggttaa tcgcctcaat ggggtgcgcc
    10561 agtttgctgt tgatctcacc gcttgccgga aacgcagcca tccggtcgcg caccagttcg
    10621 cccagcgttt ttcctttcag gcacaccagt tcggcgacca gcagccacgg gatcatgccg
    10681 ctgtcgcagt aagcgaaatc acggaagtaa tggtgggcgc tcatttcgcc accgtagatg
    10741 gcgtcttcct tgcgcatacg ttctttaata aaggcgtgtc cggttttcga catcaccggc
    10801 gtgccgccag cagcagttac cacatcaacg gtgttccagg agagacgtgg atcgtggatg
    10861 atcttcgcgc cgggattttt ttcgaggaat gcttctgcca acaggccgac aatgtagtag
    10921 ccctcaataa actgcccttt ttcgtcaaac aggaagcagc gatcaaaatc accgtcaaag
    10981 gcaatgccca tatccgcgcc gtgtttgatg accgcattgc gggtgtcgtc gcggcattct
    11041 ggcagtagcg ggttaggaat accgttgggg aaattgccgt ccggcgtgtt gtgtactttg
    11101 attaattcca ccggtgcgcc gagggcttta aatcgggctt caatggcgtc caccaccgga
    11161 cccgccgcgc cgttcccgga gttaatcacc agcttgagcg gcgtgaggtt tttgacgttg
    11221 atataactga acaggtgatc aacgtaagcg tcacgcaggt tgatttgctg atagcgaccg
    11281 cgtttggttt catcgacggg aggaaagtcg ttggcttctg ccagacgctg gacgtcgcgc
    11341 agtccggtat caccgctgat cgggcgagcc ccttcgcgca ccagtttcat gccgttgtaa
    11401 tccatcggat tatggctggc ggtaacttca atgccgccat ccacaccgag atggaacgtg
    11461 gcgaaataga tctcttcggt gccggacatg ccaatatcca gcacatcgac gcccgcatcc
    11521 tgcaaacctt ttgccagcgc cagttttaag gtttcgctgg tgaggcggac atcaccgcct
    11581 aacacaatgg ttttcggttt gagaaattcg ccataggcac gcccaatacg ccaggcaata
    11641 tcttcattca gttcttcgcc taatttcccg cgaatatcat aggctttaaa gcaggttaat
    11701 tttttcatat cgttaccctt tttcaggcaa tagttggccc tgaccgaaac ggccaatttt
    11761 gtttttgtcg ttatttacac cgcggtttcg cattcattgc ctgatgcgac gctgccgcgt
    11821 cttatcaggc ctacaaaacc gaaccgtagg tcggataagg cgttcacgcc gcatccgaca
    11881 aacagcgcct gatgcgacgt ttacacccgt ccgtagcgat ccgcgaaacg caccacatca
    11941 tcctcttcga gataagagcc ggagcgtact tcaattaaat cgagcggaat tttccccggg
    12001 ttttccaggc agtgcgtcgc ccccagcgga atataaatgg actcgttttc accaagcagt
    12061 ttgatatcac cgtcaatggt gacttttgcc gttcccgcaa ccaccaccca gtgttccgcg
    12121 cggtgatggt gcatctgtac cgacaagcct tcgcccggtt tcacggtgat gcgtttcacc
    12181 tggtagcggt cgcccgcgtc gatagagtca tatttgcccc acggacgata cacttcgcga
    12241 tgcacccgat gctcatggcg accatcggct ttgatctgct cgaccacttt tttcacatcc
    12301 tgtaccgcgt tacggtcggc aatcagcacc gcatctttgg tctgcaccac taccagatct
    12361 ttcaccccga cggtggtgac caggccagat tcggcataca catagctgtt ttcagttttg
    12421 tgattaatca catcgccgtg gcaaacgttg ccctcggcgg tgtgggcgct gatctcccat
    12481 aatgaagacc aggaaccaac atcgctccag cccgcatcca tcggcaccac aacagcatct
    12541 gccgtacgtt ccatgaccgc gtaatccacc gactcttccg ggcaggcgag aaacgcttct
    12601 tcatccacac gaataaaatc aagatccgga tcgacggcgc tcatcgcttt ttcacaggca
    12661 tcgagaatat ccgggcgata ttttttcagt tcttcgagat agcgtccggc gcggaacagg
    12721 aacatgccgc tgttccagta atattcgccg cttgccacat aggcctgggc ggtttccaga
    12781 ttcggttttt cgacaaactg cgccacttca aaggccaccg tatcctgctc acccgcagac
    12841 acttcaccgc gacgaatata gccataaccg gtttctggta gatccggcac aatgccaaag
    12901 gtcaccagct tgcccgcttc ggcatatggc atggcattac gcacggcggc acggaacgcg
    12961 tcttcatcgg caattacatg atccgccgcc agcaccagca ttaacgggtc gttctccggg
    13021 ctatgacgtt ttgccgccag cgccgccagc gcaatggccg gtgcagtgtt acgccctgca
    13081 ggttcgagaa taatgttctc ggtaagtttg ttcagttgac gcagctgttc cgcgacaata
    13141 aagcggtgct gctcattgca aatcaccacc gggctttcgc actccacgcc gttcaggcgg
    13201 cagatggtgg tttgcagcat ggtgagatcg cctttcaggc ataaaaactg cttggggtaa
    13261 agtacgcggg aaagcggcca taagcggcta ccggagccac ctgccatcac aactggatag
    13321 agtttcgact gcgccataat tatccccgaa tatcatttat aaattgacgt aacacgttct
    13381 ctttatcgag cgtgcgttcg gcatattcac gtgccaccgt gttgtacttg ggcagcagga
    13441 gcgcctgacg aatccccgcc accagcgcct cgaccgattc cggttccacg caaaccgcaa
    13501 tgcccggaaa ggtttcgcaa agctgtccca gttctgtgtg ggcctctgcg gtaatcaccg
    13561 cgttaccgcc tactgccaga atattggtca gtttcgacgg caatacggca tctgccgcgc
    13621 cgcgtttttg caccaccaga tggcaatcgc ccatcttcag cagtgcgggt aaagcgtcat
    13681 acgattgcag cgggaaaaat tgcatgttgc gcagtccacg ctgctgcgcc attttttcca
    13741 gccgcgcttt gccgccgccc tgcccgacaa tggcaaaaat cagcggttca tcgcgcaggc
    13801 gatcggcagc ttcaataacg ttttccagtc cctgcttttc accaatattg ccggagtaaa
    13861 gaatgatttt tttgttatcc ggcaggccaa gctggttacg aagggcatca acatcggcgt
    13921 ctgcaacatg ctgaaaacgg gcgatttccg accagttggg gaagaaaatg atgttatccg
    13981 ccgccacgcc tttttcgatg gctttattca tcatcgaacg cgaaatcgtg gagacgttat
    14041 cgacgttatg cagtccgcta cgttcgaacg ccgttgccag ctgtgccact ttgccgcctt
    14101 tgccttttcc ggcaaggccc agccccagca tggcgtccac ttcgtaatcc tgaatatgca
    14161 gcacggtacg cgcaccagag agtttcgcca gcaggcgcat tcccggcgtg caaaacagcg
    14221 ttggcaccac gccgataatg cgatccggct tccagcgacg ttgcgccatc aacgggaaaa
    14281 aactgctgac ggcaaaactg ccgagatgca acaggcgttt cagggtgctc ggctgttttg
    14341 gcacgtacag cgggcagcgc cacaccgtgg cggccccctc ttctcgtttg tagcgccagg
    14401 cggaatagtt ctcgcccacc tgccactgcg ggtagtaagg cggtgcggta ataacccgca
    14461 cctcatgacc ttgcgccgcc agccattcca ccatttctcc ggtgtatttg ccgatgccgg
    14521 ttaactccgg cgagtagtta atgccgtaga ccagtatttt cataatccgg gtactccggc
    14581 acgcttctca gcgagaaaat aggcgcggct gttagcgtga acattgtcac tggcgagcag
    14641 tgcgtccggc gtcagccagc ggtaatcgtc atgctgctca tccggcagta acagttcttc
    14701 ttccgctact ctgaagcgaa aaccgagcac cacatagtga gtggtgaaat ccgtgccgga
    14761 gaagttatcg tcataaaagt gctgccagac accgtaaaac tggcctgctg ttatcggcag
    14821 acgcagcccc agttccgcca tcgtcagccg ctcaaatgcg gcttccagcg tttcgtcttt
    14881 ctgcacgcgc cctcccggca caaaccagta accctgcgcc gggcggttgg ttcttttgcc
    14941 aagcagaaac tcgccgcgac tgttctcgac aataaagtcg agagagacaa gcggtgtgga
    15001 gcgcactaca gtggcaaagt cttcctgacg taaaaacatc attccccccg aaagcggtct
    15061 tgattctcaa ggaaccactg gtaagtgctg gcaagccccg cttccagtga gatttcgtga
    15121 taccagccaa gctgatgcag gcgcgtcaca tccagcagtt tgcgcggcgt accatccggt
    15181 ttgctggcat caaaaaccac ccgacctttg taacccacca ctttggcgat ggtttgcgcc
    15241 agctcgcgga tagtgcagtc aacgcccgta ccaacgttaa tgtgcgacag catcggctgg
    15301 gtgttctcca gccagacttc atgcgccagc tccatgacat gaatgctcgc cgccgccatg
    15361 tcatcgacgt gcaggaattc acgcatcggt gtaccgctgc cccataccac cacgtccggc
    15421 gcattctgtg ccgtcgcctc gtggaagcga cgcagcaatg ctgggatcac atgcgagtta
    15481 ctcgggtgga agttgtcatg cgggccatac aggttggtcg gcatgactga gcggtaatcg
    15541 cgtccgtact ggcggttgta tgattcgcac agtttgatcc cggcgatttt ggcaatggcg
    15601 taaggctcgt tggtcggctc cagcgtgccc tgcaataact cgctttctgc catcggctgt
    15661 tttgccagtt tcgggtagat acaggacgat ccaagaaaca gcagtttgtt cacgtcgttc
    15721 tgatgcgcgg cgtgaatgat gttgctctca atcatcatgt tctggtagat gaaatccgcc
    15781 ggataggtgt tgttggcaac aatgccgccc actttcgccg ccgccagata gacctggtca
    15841 atgcgttcgc tggcaaagaa atcatgcacc gcgcggctgt ccagcaggtt cagctcgtcg
    15901 cgggtgcgta ataccagttc cacatcaccg cgctgttcga gctgccgcct gatggcagaa
    15961 ccgaccattc cgcgatggcc agcgataaaa atgcgttgtc tactcatgct tatgactcca
    16021 gcgcgatcgc cacgtcgtag ccgtgagatt tcagcagaga gtgttttttc gccgcttcga
    16081 ggtcattagc caccatttca gacaccatct ctctgagggt gatttccggt ttccagccca
    16141 gtttttcgtg cgctttggtc gggtcgccga gcagcgtttc aacttcagcc ggacggaagt
    16201 aacgcggatc aacggcgata atcacatcac ccggtttaac gcccggcgcg tcatgcccgg
    16261 tgacggaaac cacaatgccc ttctcttcaa cacccgtgcc ttcaaagcgc agtttgatgc
    16321 ccagctgtgc cgctgccatt tccacgaact gacgcacgga gtactgaacg ccggtcgcga
    16381 taacgaaatc ttccggctgt tcctgttgca gcatcatcca ctgcattttt acgtagtctt
    16441 tggcgtggcc ccagtcacgc agggaatcca tattgccgag gtacaggcac gactccagcc
    16501 cctgggcgat gttggcgatt gcgcgggtga ttttgcgggt aacgaaggtt tcgccgcggc
    16561 gcggggattc atggttgaag agaattccgt tacaagcgta catgccgtag gattcgcggt
    16621 agttaacggt gatccagtag gcgtacagtt tggcgaccgc atacggagat cgcgggtaga
    16681 acggcgtggt ttctttctgc ggaatttcct gcaccagacc gtacagttca gaggtggaag
    16741 cctgatagaa acgggttttc ttttccagac cgaggaagcg gatcgcctcg agcaggcgca
    16801 gcgtacccat cgcatcgacg tctgcggtat attccggtga ctcaaaagaa accgcaacgt
    16861 ggctcattgc gcccaggttg tacacttcat ccggctgcac ttcacgcaaa atgcgcgtca
    16921 ggttggaggt atcactcagg tcgccataat gcagatggaa tttcgggttg caggtgtgcg
    16981 gatcctgata aatgtgatcc acgcgctcgg tgttgaatga cgatgcacga cgcttaatac
    17041 catgcacctc gtaacctttt tccagcagaa actctgccag gtaagaaccg tcttgtccgg
    17101 ttacaccggt gatgagagcg acttttgaca tgtcttattc ctctgtattt ttgaatttat
    17161 tcagtttcaa cgcgttcgcg tatcaccact gcgggattcc cccggcaaac cacatttgcc
    17221 ggaagcgatt taaaaacact gcttcgtgca cccacgacgg tgccgtcgcc gattgtgacg
    17281 cctggggcaa caaagacatc ggttgccagc cagcatttct cgccaatcac aataggcgtg
    17341 gcgttaatgg tgaaatgttg acttgcatgg tcgtggctcc cggtgcataa ataacttttt
    17401 tgcgatatca ccgaatgtgc gccaatggtt atttcaccga gggtatataa attgacctca
    17461 tcgccgaccc acgcgtaatc acctaaggtt aatttccacg gataggtaat ttttactgac
    17521 ggacgaataa ctacgttttt tcctattttt gctccgaata aacgtaataa aaaagcccgc
    17581 cagcgataca atacttgtgg cgaccaggca aatattgttg cctgtactgc ccaccataat
    17641 tgcactttaa tagcgttgcc gccccggaac cctttcggca ccgagaatcc acttaaatcc
    17701 tgcatcgttt tcccttatat tcagactttg ttatataagg ctttcgtctt tgaggtagta
    17761 cgttggcgta aatgccagga taattcagcc caaaagccag gtacatgtaa tatttgtcgt
    17821 tggacttttt tcgcgtcagc acacaattcc atattattgg tggtagatac cccacccatg
    17881 gaaaattcag acaccaggcc attgagtttt ttaaatgcat aaccggcttt atacattttg
    17941 gctgccagcg cgtagtcgga agaaacttta tattccaggt cataacgcca tttcttcaag
    18001 ccggataccg ggaaaaatat cgcctgatga ctggcgggca ggctgtgata aatataccag
    18061 cccggtttgg cgctacgttt aattttatgc ccgtcgccaa aatccagcag cgcatcgccg
    18121 gtgatcatca cgttatcttt ttgcattttt aacttacgga caaaatttgc ggcattctga
    18181 tgaaaaatat cgcccgaatt gagaaacaac gcgaacttgc cttgtgccat cgtaatgcct
    18241 ttgttcatgg cgtcatagat accgttatct ggctcgctga caaagcgtag gttatagata
    18301 ccattgagat tttccagata ctcacgggtg ccgtcattgg aaccgccatc gacaacaatc
    18361 cattcgaagc tgatatcttc cgcctgcgcc agatgcgcca gcgaggcatg tgttttgact
    18421 atcccttcga ggttacgaaa cgcgacagtg attatgctaa gcaacatgtt cttattctta
    18481 cctcgtaata tttaacgctt ttcgcaaaat aaacggacag acgattaaaa atgcatattc
    18541 cgggctaaat atcgaaccgg taaaaaacag cgataccgga gtaaaaagat ataattgcac
    18601 gcgaaaattg cggttatcac caaaagcgtt gatcatcatt tttatcactt tccccatgta
    18661 ccacagcgat aaaaacaccg cgaaccagga aaaataaata atcagcagat acaaaccatt
    18721 gtctatggtt tttccgacat ccgcaccgtt aaatattccg aatgatgcga catattcata
    18781 aagtgagcca aatctgacta caccgtcaat atgggtcaaa gaataaccga ccatcaccag
    18841 cggaccgaca atacgataat acgatgacga tccttccgta cctaaatcgc ccagacgggt
    18901 ggaaatataa ggaaacgcga ttaccacacc aaccaggaat acagccagag aaatcaatgc
    18961 taacggtaac tttttcttaa tcgcctcttt attcagatat tggaataccc actccagcaa
    19021 ataaaacagg ataaaggtca taacccctga aaacgatccg gataatatta tccctgcgag
    19081 aatcatagca tcggttttag gcgttttgat accaaactgt ttgatgctga gccaaattga
    19141 gattaatgcc agagcgaaaa atgccggttc gaaataaagt gctgtcgtgc gcttgccgcc
    19201 gaatttgatg aaattcagca catagctgtt gctgtaaatc agatatttcg aaatcgactc
    19261 cataatactg ctgccgccgg tgaggataat ttgcgccatc tccaccgccg ccagcgccac
    19321 caccagcccg accaccagat aaaagaaacg taatatcttg cgatggttgt gtggcgaaat
    19381 tgttttaaaa cgaatactcc acaccatgcc aataatgatc acaatataga caaacagcat
    19441 ggttgaagtg acgtatttac cggcatccag cgactgacca aacaggtagt taaacgccgt
    19501 gagtcccgca ccaatcccta aggcaatcat caatttttta acgctgatgc gttctaaaaa
    19561 cagcagtaac aagacaggta aaaaagtgac gatagtgatg gggaaacttt cgccaagctg
    19621 ggcgattttg acgttaacca gcaggtagat cagcggcagc agcaggtagc tacagattct
    19681 gatagaagtt gacatactcc tccagcatct gttgtccact gtaggcggcg cggctgcgtt
    19741 ggctgaactc agccagcgtg gtaccaaata tcgcctgcgc gatttccggt ttgcttaact
    19801 gcaccagttg cagcacctct tcttcactga cggttttacc gccggatttt tgcagcactt
    19861 cacgcgccgc atcgctatgg gtggcaatca ccggcacgcc aatcgatagc gcctcacaca
    19921 aaatcagcgg gtagttatcg acgcgagaac tgaacaccag cgcatccatc tgattgagcg
    19981 cgctcatcag cttacgtttg tcggtttcaa agccgtgatt aaccacgtta ccagcggtga
    20041 acggcgagaa cttaccaaag gtatgcagtt cgattttgtc gcccagcgcc atcatctcac
    20101 gcaccagttg ctggttagtt ttgccgtcgt aacgcaggtc atgcgccacc accgcgattt
    20161 tcggcttacc ctgggtttcg cgcaccggag gcagatccgc cagaatcgct tcggttgcca
    20221 tatcaatacc gttattgata atccggcaac gccctggacc gtacaggcta ttgaaagcgt
    20281 cagccacatg ctggctgggg gaaataaact gacagcccag cgccagcatc tcacggaata
    20341 actggcgttt gcccgccacc agttggtgtg cgcgatcaat cttcaccggc ggataattat
    20401 ttaaggtcgg gcatttctgg cagcccgttt tccagccttc acaaccgtcg gtaaaggcgc
    20461 agcgtccggt aacgctccag tggtcgtgca gcgtccagac cagagtgacg tccggtttat
    20521 ggtttttcac cttttcgcaa aagcgcacca cgctcttaag atttagccag tagctgtgca
    20581 gcacatgaaa atgcaggacc accggacccg gtgtacgagt aatggtgcga tataactcat
    20641 tgaaattgcc aaacagatcg cgattaaaca gacgaaacag gacaatattc gccattgcag
    20701 tcatccgcgg cgtatgtttg atgacctgcg gatagttttg atggctgacg ctctctttgc
    20761 cgcctttgcc gtaaccgtag acaaaatgtg acgctagccc ctgttgcagc gcacgctggt
    20821 ggagatctaa cgccacacct gctgccccgc cttccgccag tcgcacatta aattgcaaaa
    20881 tattcattta attaccttca ctcgcgcttt ttctcccacc accagcgcgt tgtccgggac
    20941 agagtcgagc accacgctgc ccgcacccac ggtgacgttg ttaccaagcg tgatatcacc
    21001 aagaataata acgttggcac cgagttcgac gccgttgcca atatgtggac atgccatgtt
    21061 atcggcacca cgattgccga tagtgacgcc gtggcgaatg gtgaaatcat cccccgccac
    21121 cacgttttta ttgatcacta cggcgtaacc gtgatggata gtaaagcggc ggccaatggt
    21181 cgcggcagcc tggatttcat aaccgaaaaa gcattcggtg ataatgcgat acagcaccag
    21241 cagaggggcc gcccaaatat tgttgaggac gttctttttg cgccacaccg aacaaaaatg
    21301 agcgacgcga taggctagaa ccatgcagca cggacgtaaa ctccagctgt tggcgcgcag
    21361 atcttccagc atctttagcg cccccgaata ccatcagcca gacgtttgcc gttacgcacc
    21421 gacagcagcg tcagcaaagt gcgccaggtc atacgtttat tgcggatctg gtagagggta
    21481 aacagctgat attttttgct ggcgcggtcg aatttgtctt tgtgcttgcg gtaaaagtgg
    21541 aagtatccgg agaatttttt cggtgacgag gtgatctgca tttcaccgtg attgatatgc
    21601 aggatctgcg tcgcctcttc cactttccac ggttcgccat actccaccac catccgcagg
    21661 aagatgtcgt aatcctgtgc cgctttcagt tcggtatcga acaagcactc tttgaaacgc
    21721 cacgcccagg taaaaacctg attgccaatg atattgcgtt tatagaacag gcggcgtgag
    21781 tacggcgatt tgggatacag tggtaagctt gctggttggg aataaacttc tccctggcat
    21841 acgtagtcgt tagcgtacaa aaaggcgtga gtgaccagtt gctgtttatg ggcgaggaag
    21901 acgctcaggc ggttgggcgt ccattcatca tcgtcatcga tcccggtaat gtattcccct
    21961 tgcgccagca taatcgcctg gttacgtacc gcgcacgccc cgctgttaat gtcgttgtga
    22021 atgtaggtga tacgcggatc gttgagggca gtgacgtact gttgcagttg ttcccaggag
    22081 gtggagcaat catccacgat gatcatctcc cagttgctgt agtcctggcg caggaccgat
    22141 tttatcgccc gaatcgccag ttgttggcgg ttccaggtcg gcatatagat tgaaatcagc
    22201 ggattgtctt tcataggttg ttctcccgga gtatcgagat gttctgccgg tttataagct
    22261 tgtttacggg ttctgttgcc ggatgcggcg tgagcgcctt atccggccta cggagctggt
    22321 ttgcttgccg gatgcggcgt gaaggtaggt cggataagat gcgtcagcat cgcatccgac
    22381 agctgatgtc gttatttcgc atccgactta tattcgtatt cgtaatagcc ataatcctga
    22441 tacgcgctgg cgcggcggaa gatggagttc agaatcaccc ctttcaccgg aataccgttt
    22501 tgctcaaagc ggctcagact ggtttccact tctttcaatg tgttgaccgc ataacgcgcc
    22561 accattaacg tggttccgac atgacgacca acaattgccg catcggtcac tgccagaatc
    22621 ggcggcgtat caatcaacac caggtcatag tttttactcg cccagttcac cagttcggca
    22681 aagcgttcgc tcatcaacag ttcagaagga tttggcggta cctgaccgcg cgggatcagg
    22741 tcaaatttgg caatagaggt cggtttagct gcggtagtaa tatcgccctg gccaatcaga
    22801 atttccgaca ggccattgac gttattggta cccaacagct cgtgggtgta gcctttgcgc
    22861 atatcgcagt cgatcaacaa cacccgttta ttggtctgac tgatcaccgc cgccaggttg
    22921 gcgcagacaa aggttttacc gattgacggg ctcacccctg tcatcatcaa cacattgttc
    22981 tgcgcctgca tcatcgcgaa gtgcagactg gtacgcagac tgcggatggc ttcaatcgcc
    23041 agatcggttg gattccccac cgccagtagc tggctctgtt tatagcgttt aatccctttg
    23101 atggttttga cgctatcgcg cgctttctgc cattccgaca acgggatgct ggcatagacg
    23161 ctgataccat gttcttccag cacctgcggg ctttcgatgc cgcgattaaa caacgagcgc
    23221 agcagcacac ccacgataga gagcatcagg ccgaggataa ttgcgccgag gataatcagt
    23281 cctttcttcg gtttcagcac gccaggctgg gtgattgccg ggtcaacaat gcgcacatcg
    23341 ccgacggtgc tggcttcggt gattttcagc tcctgctctt tattcagcag ttgcatatag
    23401 acctgctgac cagactcgac atcgcgggtc agacggacaa tttcctgctg ggttttcggc
    23461 atcgccgtta cgcgaccatt aagtttggct ttttcgtctt ccagcgcctg acgtttctcc
    23521 agcagcgtgc ggtacgcggg gtgaactttg gtgtacagct tggagatttc cgcctcttta
    23581 aaggtcagtt cgttcaactg cgcgtcgatg ttcaccatcg aatcgagcac cgctttcgct
    23641 tccagcggca gatcaacaga atctttatcc tgacggaagg cattcagttt gttttcggca
    23701 acatcgaggc ggctacgtac ttccggtaac tgttgcgcga ggaaagcgag gcttttcgac
    23761 gcttccgccg atttgcgctc aatattttgt tcctgatagt tacgggcgat gctgttaaga
    23821 atgtcgcgga tctgttcgcg atcttcaccg gtataagtca ggctcagtac gcctgcgtct
    23881 ttgccgttct ccgttaccgt caggctgttt tgcagttggt tgatcatccc caacgtggag
    23941 tatttggtga cggtaaactc actgcccggg cgggcgtgaa tggcttcaac catcagcgtg
    24001 acgccttctt ttttcagaat ctggcccgct tgcccacggg cgctaaagcc gccatcactg
    24061 ctcagagtgt agtttttgtc atccagcaca ttaagcgtaa acacctggtc cgccatctct
    24121 ttcgggcggt taaaggtggt cactttcacc gtctcgttct gacgtcccat caggcgatcc
    24181 caacccgcac cgaaaatcgg gaacgtgttt ttgctcaccg caatatcgag gtctagatcg
    24241 tccaccgttt taccaagcac cagacgcgaa cgaatcaact ggatctcggc gtccgatgca
    24301 ggcggtttgt tggctaatgc cgagccaatg tcctgcacta acgaattgcc gctgttttgt
    24361 tcgatttgta ccagtgcgtc ggcactataa atcggcgtgg cgaagaaagt gtaaaccaca
    24421 gcacagaggg caaatacagc ggtgatgcca atcacccacc agcgcgcttc aatgacggtg
    24481 ccgaccaggc gaccaatatc gatttcatca ctgcccgtta ccggagcggc atgttgtttt
    24541 actttttctg tcattcttat acctgctctg cgttcaatgc ctgctcccac tggcgggcag
    24601 accgttcaag taatgtgtac accgctgcaa acgtttcccg gcttttgcga tacggatcgg
    24661 ggatttcaca ttcgttatcc cagtgaccaa acagcatcac tttgccgcgc atctcaggtg
    24721 ccatctcgca taagcgttcg atatggcgct tttccatggt caaaatcagg tcatagttgc
    24781 gacacaggcg gcggctgatt tgccgtgcac agtgaccttc cagagacagt tgatgttctg
    24841 cggcgacgct gatagcggta ggatcagcgc ccttaccgac cagcgcgccg agtccagcgg
    24901 actccacttt cagctccggg tgataacgtt gcagtaagcg ttccgccgtc ggggaacggc
    24961 aaatattgcc gacacagaca actaagatgt tgttaaacat gacgattacc agttatgaat
    25021 gtcgctggct gtatccgtca tgtaacggac accgctaata gttggcagca actgattgat
    25081 cagacggttc cagcgggaaa ccggggcggt ggtgacatac accacgtcat aaggttgcag
    25141 gcggaattct gtcgccatca ccagcgacgt cgcatcggac atatccagct ggtagatatt
    25201 ggcaatcttg ccgttacgcc cgccctcgcc tttcagcgga cgaatgacaa agatgccgct
    25261 ggcgttggag gtggtcatgt cgataccttc ggcattgccc agggcttcag tcagggtcat
    25321 gccgctaaag tccattttca gggtgctctg tttcttcact tcacccatca caaacacttt
    25381 cagatcatca ttacgcggca cgtagagaat atcgccgggg taaagcagtc ggttctgatt
    25441 gaggtcgccg ttttgcatca gcgcctgcaa agaaatgcgt tcttcacgac cattgtgtgt
    25501 tagcaccacg ttgcgccagt cagcggtgtc ggtcaggcca cctgcggcgt tgatggcgtc
    25561 gagaatggtc agtggtacgt tggtgatcgc ctgttgaccg gatttattca cctgacctga
    25621 gatataggcc ttttgcgagc ggaaggcggc gatattaaca tccacctgcg ggtcagcgat
    25681 gtacgtcgct aagcgcccgg taatatcact gcggatttca gcgagcgttt tcccgactac
    25741 gtggaccttg ccgatatacg ggtaaaacat agtgccgtca ggctgtaccc agttgccggt
    25801 gtcgctggag ctgcggtact gaccggctgg cgtggtgagt tccgggtgat cccagacggt
    25861 gacattaaga acgtcccccg gcccgacgcg atactggtaa ttcgcgatct cactttccag
    25921 cgtcatattg gggcgcgcta cattcgggcg tggacgtaat tgatcaatca ggcgcggggt
    25981 caacggataa acattcacca ttttgtcgag atcgaaatca gcgtcctgct gtttgatgac
    26041 gtctttgccc atcgtcgaca tattgctgcc cggaagtact gtgcaaccgc ttatcaaggt
    26101 tactgacacc aataatggca tcaatttcat tttggatttc atcattgttt atttatcact
    26161 ttggcagagt aattatcctg tgcactatta atagcaatgt cgccatgcac atttaccttg
    26221 cagttaattg aataaaaatt taactggcat cagtcctaaa aaaattgatt tcatccgcag
    26281 gctattgaca gaataattca gactggtctt tcaggcatcc agacacgcta ccgcccctgg
    26341 ctttttagct accaatacac tgatttagct taatttttca aaccctctct gcatacaatc
    26401 gttgatgaga aagggttatt gcggaaatta acttccgaat ataaggtgac attatggtaa
    26461 ttgaatattg gctctccaat aatgcaggag gaagtgttac agctaacgga atagcaggca
    26521 aggtaacaat tcggcaattg gctattttta agaattatat taagtgtcat tcaatatggt
    26581 ttttaggagt ttctttaggt tgacaatatt taatattgcg cctccacatg cgatatttct
    26641 taaataatgt tttattatta ccacttttaa ttcagggata atgtgaggtt attaccctca
    26701 aataatatga gataaatagt gctgcaacat tgcatttttg ccccgattta tccatgatcg
    26761 aattgtgaca tttgtcatac aacgaatagg ttttgtactt actatggaat ggattgccga
    26821 tccgtctatc tgggccgggt taatcacgct gattgtgatc gaactggtcc tcggcattga
    26881 taacctggtc tttattgcca tcctcgccga aaaactaccg ccgaagcagc gcgaccgcgc
    26941 acgggttacc gggctgctgc tggcgatgtt aatgcgcctg ttactgctgg cgtcaatctc
    27001 ctggctggtc accctgacgc aaccgctgtt cagcttccgc tcgtttacct ttagcgcccg
    27061 cgacttaatc atgctgtttg gtggtttctt cctgttgttc aaagccacga tggagctgaa
    27121 cgaacggctg gaagggaaag acagcaataa tcccacacaa cgcaaaggtg cgaagttctg
    27181 gggggtggtg acacaaattg tggtactgga cgccatcttc tcactcgact cagtgattac
    27241 cgccgtcggg atggtcgacc atttactggt catgatggcc gccgtggtta tcgctatcag
    27301 cctgatgttg atggccagca agccgttaac gcaatttgtt aacagtcacc cgaccatcgt
    27361 tattctctgc ttaagcttcc tgttgatgat tggctttagc ctggtggcag aaggtttcgg
    27421 cttcgtcatt ccgaaaggct acctgtacgc tgccattggt ttctcggtga tgatcgaggc
    27481 gctcaatcag ttggctatct ttaaccggcg acgttttctt tccgctaacc agacgctacg
    27541 ccagcggact accgaagcgg taatgcgcct gcttagcggg caaaaagaag atgcggaact
    27601 ggacgccgaa accgcgtcca tgctggtgga tcatggtaac cagcagatct ttaatccgca
    27661 ggaacggcgg atgattgagc gggtacttaa tcttaaccag cgtactgtca gcagcattat
    27721 gacgtcgcgc cacgatattg agcatatcga tctcaacgcg ccggaagacg agatccgcca
    27781 gttgctggag cgaaatcagc atacgcggct ggtggttacc gacggtgatg acgcagaaga
    27841 tttgctcggt gttgttcacg ttatcgacct gctacaacag tcactgcgtg gcgaaccgct
    27901 caacctgcgg gtgttgattc gccagccgct ggtgttcccg gaaaccttgc cgttgttacc
    27961 tgccctggag cagttccgta atgcccgcac gcactttgct tttgtggtgg atgagtttgg
    28021 ctcggtggaa gggattgtga cattaagtga cgtcactgaa accattgccg gtaacttacc
    28081 gaacgaagtg gaagagatcg acgcccgcca tgatattcag aagaatgcgg acggttcctg
    28141 gacggcgaat ggtcatatgc cgctggaaga tctggtgcaa tatgtgccgc tgccgctgga
    28201 tgaaaaacgt gaatatcaca ccattgccgg gctgttgatg gaatatttac agcgtattcc
    28261 aaaacctggc gaagaagttc aggtggggga ttatttgctt aaaacgttgc aggtggaaag
    28321 ccatcgcgtg cagaaggtgc agattattcc gctgcataag gatggcgagt tggagtacga
    28381 ggtgtgatgg tatcggtcat tttacccgat agccgaaagg aataagatga gggaaccgcg
    28441 ccagcggcga tttaccgccg ggagcccggg ttgccagggt ggtggcgatt gagccaccct
    28501 ggcacgttca ccggcttgag cgttccatag tagtgaggaa catcaagtga acggaacagc
    28561 cttcgatctt acatattcag aatataatgg tgccgccgcg aacgcctgat gcgacgcttt
    28621 acgcgtctta tcaggcctac aggacaggtc acctggtaac ctacatcttc tccagcaact
    28681 tcttcacatc tttgccattg cgggaatctt tattccgctc ggcccagtca ttcaggcgac
    28741 gtttcgcttc gtcctgtaga tgtttgcgca gtaactgatc cacttgcaga ctgtaattga
    28801 gttgctgcca gttgccataa acccgcagcg gtactggcgt ttctttgagg aaatcaatca
    28861 gtttgctttc cccgttccag ccacccacga cccgaatatc aaactgggtg tcgcaggttt
    28921 gatctgccag attcaacatg ccttcccctg tcagcgccag cacaggcgat tgaccttgca
    28981 tgtcgtttaa cgtcacgacg ccatccttca acgtcaaatc ggtggtaaag cggtcaagcc
    29041 gcgtcacgtt atcaaagttt tcagcggcct tcacatcacc gccattacgc tctaccgctt
    29101 gctgaatcat ctgctggaag ttcatccctt ccatgcgcgt gtcggtcatt tcgacatgtg
    29161 cctgtccttg ccagttgtgg cggaatgcgt cggcatctat gtcagcaccg gagaaatcac
    29221 cagccagtga catttttccg gtcagcgaaa tggggtagtt aaaggcattc agaatggtac
    29281 caatctcaac gttttccagc cgtggctgga agtttatccg cggatttatt gatgtcgcgt
    29341 ccagcgtgcc cggcagtgaa acctgtccac cgttaagctt gccctgcagt tgagtaattt
    29401 ccagcaaacc cgacttgttg gtcatttgcg tggcaacatc tgtaaaattc attccgcgcc
    29461 agcgcacgtt actggcctgc aacaaaatat cagccgtaaa gccttgcagt ccctgatatg
    29521 ccggttcatc aatacgcgaa gaaatgaccg ggcgcggcaa tgttgattgg ctctgcccct
    29581 gctgcgcggc accgttttca ccattcgctg tttcattaag cgggatgagg ttgtcaagat
    29641 tcagttgcgg gaattgcagt ctcagctgcc attcaggttt ctctgtcagc gtgacctgtg
    29701 cttgcccgct cagcgtgctg tcattggcgg tcaagctgat ttggttaaat gaaaggcgtt
    29761 tatgtgactc ctgccactgg gcctggaaac tcccctgccc ctgaatacct tgttttggta
    29821 aatctgcacc ctgcaactgc cagttaattt gttcaatagc cgccgttaaa tcatgcggat
    29881 aatcagaagc atctaccgta ccgttaaggg atatcgtgag atcgcgctga tcgcgattaa
    29941 cccgcccgga gaactcaaat gaaccacgat gttggggatc ttgttccatt tgcaggcgaa
    30001 tattgcggat tgtcacctgc tcgtcatctt catgctggaa caccagcacg ctgtccgcca
    30061 ccttaagact ggatatatca aacgaccatc cgcgatcgtc tgacagatcc ggcaaggtat
    30121 tgtcgcgcgg tgcaaccgga gcgtcttcac tgcgcaccgc ttccgtctgc ggcgtcagtt
    30181 ggatcactgc cccttttagc atcacctgct taacgctcag ttgatgactc agtagtggta
    30241 aaagcgccac gtccagacgc atgttgtcgg cgcgaaccag tggctggctt gcgccctggg
    30301 cggtgagaga cattcgcccg gagaggatac taagctgcgg ccagacgtgc caacgcagtg
    30361 gcccgtcgag ctgcaattga taaccgctac gtgcagcaac ttgcttgacc atatagtcgc
    30421 ggaaatcatt cggattcacc agcaacacta acgcagataa cccggccacc agcacgacca
    30481 ggagtatcat cagcgtcgtc agaaatcgtc tcatggtatc ctcaatgggc ctgaattagt
    30541 ctttatcgat tcggctggct accgcgccct gctggttgcg atatttcgca tcttcacggc
    30601 ggttgtaagg tcgcgccgcc gggccggaaa gcggctcaaa gctcagcgca ccaattaaca
    30661 tgcccggacg cagcgccagc ggcagcttac cggagttgta gaactccagc acaatgcaac
    30721 cagaccagcc cggatcgata cggtgcgcgg tgacgtgcac catcagcccc agacgcgcca
    30781 aagaggaacg cccgtccagc cagcccacca gatcggctgg cagcgtcacc gactccagcg
    30841 tcaccgccag cgccagctct cctgggtgaa gatagaacgc ctcgccctcg tcgagaacga
    30901 tctcatcgct catcacgcgg tcaagcgcgg cgctcacttc atctttggga ccgctcagat
    30961 cgataaacgc tgccgtgtga ccacggaagg tacgaaattt attgcccaag cgtacatcca
    31021 ccgtcgcgcc gttaatacgc tccactggcg gacgtgggtt gatcgacaaa cggccttcat
    31081 caagccaggc ttcaatatct cggtcacaca gacgcatggc attttctcct ttcgcgcatc
    31141 actcccttaa cgccaattac gtcaagggca taccaggtta tcactgaacg gtacacaatt
    31201 tatcaagctt attcaaagaa ctgacttatt ttcgctttca atatatcgat cgcgatgcgg
    31261 tttttcccgc cgcgcggcac gataatgtcc gcatattgtt tagaaggctc aatgaattgc
    31321 aggaacatcg ggcgcacggt tttttgatat tgcgccatca cggaatccat tgaacgccca
    31381 cgctcgttaa cgtcacgctt gatgcggcgc atcaggcaga tatccagcgg ggtatcaacg
    31441 aaaatggaga agttaagttc gtcacgcaaa cgcgcatccg tcagcaacaa aatgccttcg
    31501 agaatgatga ccttcttcgg ctcaaccgtc accgtttctt tcatacgcgt atgttcaaca
    31561 tagctgtaaa ccggcaggtc aattgccgag ccgcgtttca acgcttgtaa atgctcaagc
    31621 agcaggctgt gatccatcgc gctgggatgg tcgtagttgg tcttaacgcg ttcttccatc
    31681 gacagatggc tttgatcttt gtaatagcag tcttcgggaa ttacgccgat gtgttcatca
    31741 ccgacttgct cacgtaattc acgataaagg gtactggcaa taagactctt gccggaagcc
    31801 gatgcgccag cgataccgat aatgacgcac tgatgagact gatcagtcat atatttagcg
    31861 acctgattaa cctggatgtt aggaaggggc gacgaagcgc caaacgcggc aattataggg
    31921 atttcatccg cctgatacca gtcgaatagc gctgccgcgc gctcagagtt tattgttgac
    31981 gaagaattcc cggtggcaaa ttacgttgat cagttttatg taaggtaaaa aatgttatac
    32041 gcagttgcgc aaattatccg cctttacgtc actttatgag caattcgcat ataaaatgta
    32101 aaacttttgt actagcataa acacagaaac gaatactggc gacctggtct tgcggataaa
    32161 gcggtaatga gcaaacaatc acagcatgta ttaattgccc tgccccaccc gctgcttcac
    32221 ctggtcagtt taggtttagt ctcgtttatc tttacccttt tctcgcttga gctttcgcag
    32281 tttggcaccc aactcgcccc actgtggttc ccgacgtcca tcatgatggt ggcgttttat
    32341 cgccatgccg ggcgcatgtg gccgggaatt gcgctgagct gttcgctggg aaatatcgcc
    32401 gcatccatcc tgcttttttc caccagctcg ctgaacatga cctggacgac catcaatatt
    32461 gttgaagccg tggtcggggc agtgctgcta cgtaaattgc tgccgtggta taaccccttg
    32521 caaaatctgg ctgactggct gcgtctggca ctcggcagcg ccattgttcc gcctctgttg
    32581 ggggggtctg gttgtcctgc tgacggccgg agacgatcct ctcagggcat ttttgatatg
    32641 ggtactgtca gaatccatcg gcgctctggc actggtgccg ctgggattgt tatttaaacc
    32701 acactatctg ctgcgccatc gcaacccacg gttgcttttt gagtcgctgc tcacgttagc
    32761 catcacactg acgttaagct ggctttcgat gctgtatctg ccgtggcctt ttactttcat
    32821 tattgtgctg ttgatgtgga gcgccgtgcg cctgccacga atggaagcct ttttgatctt
    32881 ccttaccacg gtgatgatgg tgtcgctgat gatggccgcg gatccctccc tgcttgctac
    32941 gccgcgtaca tacctgatga gccatatgcc gtggctaccg tttttgctga tcctgctgcc
    33001 cgccaacatc atgacgatgg tgatgtatgc ctttcgtgcg gaacgcaaac acatttccga
    33061 aagcgaaacc cgttttcgga acgccatgga atattccgct atcggtatgg cgttagtggg
    33121 caccgaggga caatggctgc aaaccaacaa agcgctctgc cagtttctcg ggtacagtca
    33181 ggaagagctg cgcggactca cctttcagca actgacctgg ccggaggatc tcaataaaga
    33241 tctccaacag gttgaaaagc tgataagcgg tgaaataaac acctattcaa tggaaaaacg
    33301 ctactacaac cgcaatggcg atgttgtctg ggcgttgctt gccgtctcac tggtgcgcca
    33361 cacggatggc acgccgctct attttatcgc tcagattgaa gacattaacg agctaaaacg
    33421 caccgaacag gtgaatcagc aactgatgga gcgcatcacg ctggctaacg aagcgggcgg
    33481 gattggcatc tgggagtggg agttgaagcc gaatattttt agctgggata agcggatgtt
    33541 cgagctgtat gaaattcctc cacatatcaa accgaactgg caggtgtggt acgagtgcgt
    33601 gctgccggaa gatcgccagc atgccgaaaa agtgattcgt gattcgttgc aatcacgctc
    33661 gccctttaaa ctggaatttc gcattaccgt aaaagacggt attcgccata tccgcgccct
    33721 cgccaaccgg gtactgaata aagaaggcga agtcgaacgc ctgctcggca ttaatatgga
    33781 tatgactgag gttaaacagc ttaacgaggc attgtttcag gaaaaagagc gcctgcacat
    33841 aacgctggac tccatcggcg aagccgtggt ctgtattgat atggcaatga aaattacctt
    33901 tatgaatcca gtggcggaga agatgagcgg ctggacgcag gaagaagcat taggtgttcc
    33961 gctcctgacg gtgttgcata ttacctttgg cgacaatggt cccctgatgg agaacattta
    34021 cagtgccgac acctcacgtt ccgcgattga acaagatgtg gtgttgcact gccggagcgg
    34081 cggcagctac gacgtgcatt acagtattac gccgttaagt actctggacg gcagcaatat
    34141 tggttcggtt ctggtgattc aggacgtcac cgaatcacgc aaaatgctac gccagctgag
    34201 ctacagcgcc tcccatgatg cactaacgca tctcgccaat cgcgccagtt ttgagaagca
    34261 actgcgtatc ctgctgcaaa cggtaaacag tacacatcag cgacatgccc tggtgtttat
    34321 cgatcttgat cgctttaaag cggtgaatga cagcgccggg cacgccgctg gcgacgcttt
    34381 actgcgcgaa ctggcgtcgt tgatgctgag catgctgcgc tccagcgacg tgctggcgcg
    34441 gctcggcggt gatgaatttg gtctgctgct gccagactgc aatgtcgaaa gtgcgcgttt
    34501 tatcgctaca cgtattatca gtgctgtgaa tgactatcac tttatctggg aaggacgtgt
    34561 acatcgggta ggtgccagtg ccgggattac cttgattgat gacaacaatc atcaggcggc
    34621 tgaagtgatg tcgcaggctg atatcgcctg ttatgcctcc aaaaatggtg gccgtggccg
    34681 ggtgacgatt tacgaaccgc agcaagctgc cgcacatagc gaacggacgg cgatgtcgct
    34741 tgatgaacag tggcggatga ttaaagagaa tcagttgatg atgatcgccc acggtgtcgc
    34801 ttcgccgcgg atcccggaag cgcgtaattt gtggctgatt tcgcttaaac tctggagttg
    34861 cgaaggcgag attattgatg aacaaaaatt tcgtcgtagc ttcagcgatc cggcacttag
    34921 ccatgctctt gaccgacggg tattccacga atttttccag ctggccgcaa aagcggttgc
    34981 cagtaaaggc ataagcatcg ccctccccct ttccgttgcc ggtttgagta gcgccacgct
    35041 ggtgaatgat ctgcttgagc agctggaaaa tagccctcta ccaccacggt tattacatct
    35101 gattattccg gcagacgcga ttttagatca cgcagaaagc gtgcaaaaac tgcggctggc
    35161 gggatgtcgg atagtgctca gccaggtggg ccgcgatctg caaatcttca actcgctgaa
    35221 agcgaatatg gcagattacc tgctacttga tggtgagtta tgcgccaacg tgcagggtaa
    35281 tttgatggat gagatgctga ttacgattat tcaggggcac gctcagcgac tcgggatgaa
    35341 aaccatcgcc gggccagtcg ttttaccctt agtgatggat acgctttctg gcatcggcgt
    35401 cgatctgatt tatggtgatg tgattgccga tgcccaaccg ctggatttgc tggtgaatag
    35461 cagttatttc gcgattaact gagggagcgt ttgcccattg cctgatgcga cgcatcaggc
    35521 ctacaattcg tccgttccgt ctggttgcca gccttccgta taccagatat gcaacagcgc
    35581 ataagaacgc cagggcttcc agcgctcggc ataacggcgg atttgtgccg gtgtcatccc
    35641 cggaaatcgc tgtttaatca gataatcatc cggcagaaaa acatctttcg cctgccagcc
    35701 acgcaaagcg aaataattcg ccgtccagcg cccgataccc ggaaaagttt gcagcgtttt
    35761 catcgcctgc tccacatcgc ccggaattgt cattggtaag ctgccctcca gcgccgcatt
    35821 tgccagatga atcagtgcct ctgcccgttt caacggcatg cctaacgctt ttaatgcctg
    35881 cgggtcggct gctgccagcc gctgaggcgt ggggaagcag atatactccg gaaaatcatc
    35941 cagccgttcg ccataaagct gtaccacttt ggcagtcaat tttgccgcca tcgccacgct
    36001 caccagttgg cctaaaatcg cccgcacacc ctgctcaaaa gcatcaacac agccgggtaa
    36061 acgcaatccg ggccgcgccg cgcctaactt gcccaacgca ccgttaacaa tctgcgggtt
    36121 acattgcaga tcaagcaggc ggctcatttt cgccagacac tcagcggcaa caggttctaa
    36181 acctgcactt aaatttatgt gcagagtatg acgggccata tccggaatag cagtcactac
    36241 gccgcgatat tcgcccaccg ccagactacg ggcataataa tcgtcagcga ccgtttccac
    36301 gccactcact gcacgggcgg cgagaaatcc caacatccac gaccagtcat acggcggctg
    36361 ccagttcagg gtatacatcg catctcctta ttcatccgct ttcagcataa acgttattca
    36421 gacgctacgc tttgctttca tattccggtt gtcgcgacgg caacatttcg ctaaagtcac
    36481 gccccttctt caccggcatg gggattaatt cgtgtttatt ggttttgatt acggtacagc
    36541 aaactgttca gtggcggtca tgcgtgacgg taaaccgcaa ttactgaaaa tggaaaacga
    36601 cagcacgctg ctgccttcaa tgctttgcgc gccaacgcgt gaagcagtaa gtgaatggct
    36661 gtaccgccat catgatgttc cggcagacga cgatgaaacg caggcgctgt tacgtcgggc
    36721 gattcgttat aaccgcgaag aagatatcga tgttacggcg aaaagcgtgc agttcggtct
    36781 ttcctcactg gctcagtaca ttgatgatcc agaagaagtg tggtttgtga aatcaccaaa
    36841 atcgttcctc ggtgccagcg gcttaaaacc gcagcaggta gcgctgtttg aggatctggt
    36901 ctgcgcaatg atgttgcaca ttcgccagca ggcgcaggcg cagctgccag aagcgattac
    36961 tcaggcggtg attggtcgcc cgatcaactt ccaggggctg ggcggcgatg aagcaaacgc
    37021 ccaggcgcaa gggattctgg aacgcgcggc gaagcgtgcc ggattcaggg acgtggtatt
    37081 ccagtacgag ccggtcgcgg ctgggctgga ttacgaagcc accttgcagg aagaaaaacg
    37141 ggtgctggtg gtggatatcg gcggtggtac gactgactgt tcattgctgc tgatggggcc
    37201 gcagtggcgt tcgcgtctcg atcgtgaagc cagtctgctg ggtcacagtg gttgtcgtat
    37261 tggcggtaac gatctggata tcgccctggc gtttaaaaac ctgatgccac tgctgggtat
    37321 gggtggcgaa accgaaaaag gcatcgccct gccgatcctg ccgtggtgga atgcggttgc
    37381 catcaacgac gtacctgcgc agagtgattt ctacagtagt gccaacggtc gtctgcttaa
    37441 cgatctggta cgcgatgccc gcgaaccgga aaaagtggcc ctgttacaga aagtctggcg
    37501 tcagcgttta agctatcgcc tggtgcgtag cgcagaagag tgcaaaattg ctctttcaag
    37561 cgtagcggaa acccgcgcct cactgccgtt tatcagcaat gaactggcta cgctgattag
    37621 ccagcgaggg ctggaaagcg ccctcagcca gccactggcg cggattctgg aacaggtgca
    37681 actggccctg gataacgccc aggaaaaacc ggacgttatc tacctgaccg gcggtagcgc
    37741 ccgttctccg ctgattaaaa aagcgctggc agaacagttg ccgggcattc cgattgcagg
    37801 cggcgatgac tttggctccg tcaccgccgg gctggcacgc tgggcggaag tggtgtttcg
    37861 ttaattgaaa aatggctcag cagcggctta aatctgcctg cgcctgagcc agtttttctg
    37921 ctgcctgacg taactgttcc atcaacggca tggtccgtgc tggagcatga aggcggaatc
    37981 gctgcaactc tgtcgcgccg actgtcaacg ccgattccag ccgatgtcgt ttggcggtca
    38041 ttttcgccat taccgcttgt ctgtctgctg gcgttatcgc ttcattcggc ctgaacacaa
    38101 aacggcgttc acagctcgct ttccagtcga taaccgcctg ggtcagatgg tcgccaaacc
    38161 ctttcacttg cttaacgcca cgacgggtaa catctgcggc tgtttcaata ccaaaagacc
    38221 gtaacgccgc tttacgcgca gggccaacgc cgggaataga ggcaacatca ataaaaaatc
    38281 cctccagaaa cttctgcttc tgccgttccc ttgcagtgtc atgaagtgct gccagagcgc
    38341 gtttctcttc ttccggtaac ccgagaattt cgtccttcat tttttcgagc atcgtccgtt
    38401 tggcgataaa accttccagg ccgcctacct gctggatctg gcggactaaa tggtcatagt
    38461 ccattttcgc acgattaaat gcctcacggc gttgctgaac ttctgctttg tacgctttgc
    38521 ttgtcagact gccaataatc cagatagccg ccagcacagg aaccaaaata atataacgcg
    38581 gttctgcctg aaggccgcaa agcagcagcg ataatgctga cagtgcgatc tcaagcagaa
    38641 tgatgtattc gcgccgtaac aggcctaaag gaagcggcct gccagtcggt tggaaatgat
    38701 cgggtaatgg cagttgcaat gctggcggtg ctactgacgc catcaccatc gcccagactt
    38761 ttgccagcac aaaatccccg ccggtggtaa tgacctcttc gccgagatca ataaaataga
    38821 taacaccttg attatccagc gcacaccatg ggcagtcagc caaatgaccg ggataaacat
    38881 gcattgccga aacggtacat ttctttaact gttggcgcag agaatcaagt gccgctaccc
    38941 acgctttagc cgtcggacgc ccggttgcca cgccactttc cgtgaatgcc tgctgaaaca
    39001 tggcttcaac atcactcggt aacatcgata gcggaatcga tcgtggcggc ggttttaaac
    39061 cacgtcgctg attatctgac gcgtaggcat aacggaaatg ggtaatatcc gtctccagcg
    39121 cattacccgc atcagagata agcggcacac cagaataagg atgccggcca ccaaacaaga
    39181 cgtgaaaaat cagcaacgca aggccaaaat tatcgtgatt ttctgtgcgt tcaaagccga
    39241 caaatgacga caaggtttgt agctctggcg gcgtaaaatg cgacacgccg acttcgcata
    39301 aatgcagtgt gccattggcg ttaatctgaa aggagtcgct atcaatcagt accactttgc
    39361 tgtcgcgacc taccataaaa ctgttctggt ttacgtcacc cacgacgtgc ccgtgctcgt
    39421 gaaccgtagc aaaagatgaa gcaatattgc gcgcaacata gagtagaaaa tcccacgcac
    39481 aatgagggta acgttgacga cgatgtgccg ggctatagat catatgaatc ggttctttac
    39541 cagaaacttt tggcatcata aaaccgataa cttttccgcc tcgtccaccg tgaagcgttg
    39601 cctgcggcca ggcgacataa ttcaacaatt gcgcgtcagc tgtcgcagcc ataaaggcaa
    39661 gtttgtcctg ttttaaggcg ggtggcggcg tgtgataaat cttggcgacg ctatcgacaa
    39721 actcattgat atcataaacc gcgccttcac cgcctttgcc cagttcacgg cctggcgtaa
    39781 cgcactcacc agtagcagta tataaagtgg gtttcatggt aaccacagcg ccagtgctaa
    39841 cgttttatca tcatcggtac gctcgtttac cgctgggctg gacaaaaact gctttaacaa
    39901 ttcaggtaat aaatcgagtt gttcctgcgt tgctgacgcc agtccattga aaaacggggt
    39961 aaagaaaggc acatgcggag aattatccaa catatttaac gccagtcgct ggataccatc
    40021 cgtaaatgct gcaactttat gcacacgctc agtgctggtg aaagtttcca gtctggaaac
    40081 ggcatcttca tcggtaatga agtgcgtcat attggcatat tcacccacca tcggcgtaag
    40141 cggcaattgc agaccgtgac caaaatccac caccacgccg ccatcgccaa tctgcataat
    40201 caaggtgcca ttagccgatg aaatcaatcc cagaaacgtg caggcaaaat cgcgaaccgc
    40261 taattctttg gcctctgcct cggcaaaaag tcgctgacga acggtcagca caatatccgt
    40321 ggcgaggata tcattaagcc ccaattcccc accctgcact ttttgcgaca tataagccat
    40381 cgcttcattg acggcgagca tcgcgccttc gccaccctgc gagacgctgc ctgcaccatc
    40441 agcaagaaac atcaccagta atggttgctg atcgtttaac caggcaacct gcatctggca
    40501 ggcatcctga caaggtaaat cagcgctaat gtgcgacgtt ccaacagcgg atgcataaac
    40561 cagacgccag ctcacactga cgtccagcct tttggcgctt ccagcacaac ttccgttccc
    40621 ggcgtggagc gggaaaccga acgcagcgaa ctggataacc agctaaacag ttcacggaat
    40681 tgtaatcctt gcagaggcaa aggctgacga acgctgattt gtgccagcgt cttcatatcc
    40741 gccccctgaa cagcgatgga aaagaaggca aacttcttat cttcttcccc ctgaaacact
    40801 ttgttggcgg cagcctgcca ctcatcggtt ggagcgccat cggtgatcag gaaaatccac
    40861 gggcgataat acgaaatacc atttgcgcgg tactcacgtt tcctctcttc aaccatatcc
    40921 agggctttgg taatggcggc ccccattggc gtatccccct gggcaaacag gatgggcggg
    40981 aaaaagttag cggcgctggt aaagggttgt tccacatgca ccgggccgaa cgtcacaata
    41041 ccgagttcca ctctttttaa ggccagcgaa tcggcaagca gctcatcgcg aaaggtaacc
    41101 aatccggcgt taagttcatt aatgggtctg ccgctcatcg agccagaaac atccagcagc
    41161 aaaatgcagg ggcaacgtgg ttccgggtta ctggcaaaat cgctggtggc aaacgtgatt
    41221 tgttcgctca taagaaatcc tttcttagag aattattgag gtccggtatc ataccgactg
    41281 caataatgaa ataagagcaa acgaagaaac tttcagaatt aggcccggcg gcgcagaaaa
    41341 ttttgctatt cgcctcgcaa gaacggagag ccgtcgattg acactaaggg cggagtgaca
    41401 taatttcagg agtgagggtt agggagaggt ttccccctcc ccctggtgtt cttagtaagc
    41461 ctggaagcta atcactaaga gtatcaccag tatgatgacg tgcttcatca taaccctttc
    41521 cttattaaaa gccctcttct ccgggagagg ctttcccgtt tcagcgtccc gctgaaatca
    41581 tcggcttacc tcctttcgcc ctgaatgcag tctatcgcta acgcgtgaat aaaatagttt
    41641 cctctgtgat tattgcgtaa cgactcgcaa agaatatttg ctggcaggat cgcagactac
    41701 aaagcctgcg gattgacaat cttatcgtga aggcatactt tcaggagtga gggtagagcg
    41761 gggtttcccc cgccctggta gtcttagtaa gcggggaagc ttatgactaa gagcaccacg
    41821 atgatgagta gcttcatcat gaccctttcc ttatttaagg ccccttcctc gggaggggct
    41881 ttcccgtttc agcgtcccgc tgaaatcgtc ggcttacctc ctttcgccat gcaagcagtc
    41941 tatcgctaac gcgtagataa aatagtttcc tgtgttatta ctggatgcgt gctcgcaaat
    42001 gtgcccgcca ttcagacgat tcccgatagt gtttcataat tcctccattt ttctccctta
    42061 ttggctggct acactagtat cattccgcga aacgtttcag gaagagaaac tcttaacgat
    42121 gaaaggcagt tataaatccc gttgggtaat cgtaatcgtg gtggttatcg ccgccatcgc
    42181 cgcattctgg ttctggcaag gccgcaatga ctcccagagt gcagccccag gggcgacgaa
    42241 acaagcgcag caatcgccag cgggtggtcg ccgtggtatg cgttccggcc cattagcccc
    42301 ggttcaggcg gcgaccgccg tagaacaggc agttccgcgt tacctcaccg ggcttggcac
    42361 cattatcgcc gctaatactg ttacggtgcg cagccgcgtg gacggccaac tgatagcgtt
    42421 acatttccag gaaggccagc aggtcaaagc aggcgattta ctggcagaaa ttgaccccag
    42481 ccagttcaaa gttgcattag cacaagccca gggccaactg gcaaaagata aagccacgct
    42541 tgccaacgcc cgccgtgacc tggcgcgtta tcaacaactg gcaaaaacca atctcgtttc
    42601 ccgccaggaa ctggatgccc aacaggcgct ggtcagtgaa accgaaggca ccattaaggc
    42661 tgatgaagca agcgtcgcca gcgcgcagtt gcaactcgac tggagccgca tcaccgcacc
    42721 agtcgatggt cgcgttggtc tcaagcaggt tgatgttggt aaccaaatct ccagtggtga
    42781 taccaccgga attgtggtga tcacccagac gcatcctatc gatttagtct ttaccctgcc
    42841 ggaaagcgat atcgctactg tagtgcaggc gcaaaaagtc ggaaaaccgc tggtggtaga
    42901 agcctgggat cgaaccaact cgaagaaatt aagtgaaggc acgctgttaa gtctcgataa
    42961 ccaaatcgat gccactaccg gtacgattaa agtgaaagca cgctttaata atcaggatga
    43021 tgcgctgttt cccaatcagt ttgttaacgc gcgcatgtta gtcgacaccg aacaaaacgc
    43081 cgtggtgatc cccaccgccg ccctgcaaat gggtaatgaa ggccattttg tctgggtgct
    43141 gaatagcgaa aacaaagtca gcaaacatct ggtgacgccg ggcattcagg acagtcagaa
    43201 agtggtgatc cgtgcaggta tttctgcggg cgatcgcgtg gtgacagacg gcattgatcg
    43261 cctgaccgaa ggggcgaaag tggaagtagt ggaagcccag agcgccacta ctccggaaga
    43321 gaaagccacc agccgcgaat acgcgaaaaa aggagcacgc tcctgatgca ggtgttaccc
    43381 ccgagcagca caggcggccc gtcgcgcctg tttattatgc gtcctgtcgc caccacgctg
    43441 ctgatggtgg cgatcttact cgccgggatt atcggttatc gcgccctgcc cgtttcggcg
    43501 ctgccggaag tggactatcc gaccattcag gtggtcacgc tctacccagg tgccagcccg
    43561 gatgtcatga cctctgccgt taccgcgccg ctagaacgcc agttcgggca gatgtctggc
    43621 ctgaaacaga tgtcgtcgca aagttccggc ggtgcgtcag ttatcacttt gcagttccag
    43681 ctaacattac cgctcgatgt cgccgagcag gaagtgcagg ccgcgattaa cgctgcgacc
    43741 aacttgttgc cgagcgatct gcctaacccg ccggtttaca gcaaagtgaa cccggcagat
    43801 ccgccgatca tgacgctcgc cgtcacctca accgccatgc cgatgacgca agtggaagat
    43861 atggtggaaa cccgcgtcgc gcagaaaatc tcgcagattt ccggtgtcgg actggtgacg
    43921 ctttccggcg gtcagcgtcc ggctgttcgc gtcaaactta acgctcaggc gattgccgcc
    43981 ctcggcctga ccagcgaaac cgtgcgcacc gccattaccg gcgctaacgt taactcggca
    44041 aaaggtagcc tcgacggccc ttcccgtgcg gttacgcttt ccgctaacga ccagatgcaa
    44101 tccgccgaag agtatcgcca gctaatcatc gcctaccaga acggcgcgcc aattcgtctg
    44161 ggcgatgtcg caactgtaga gcaaggtgca gaaaacagct ggctcggcgc gtgggcgaac
    44221 aaagaacagg ccattgtgat gaatgttcag cgccagcccg gtgctaacat tatctccacc
    44281 gccgacagca ttcggcagat gctgccacag ctcactgaga gtctgccgaa atcggtgaag
    44341 gtgacagtgc tttccgatcg caccaccaat atccgcgcat ccgtcgatga tactcagttt
    44401 gaattgatga tggctatcgc gctggtagtc atgattatct acctgttttt gcgcaatatt
    44461 ccggcgacca tcattcccgg tgttgctgta ccgctgtcgt taatcggcac tttcgcggtt
    44521 atggtgtttc tcgatttttc aatcaataac ctgacactga tggcgttaac tatcgccacc
    44581 ggattcgtgg tcgatgacgc catcgtggtg atcgaaaaca tttcccgcta tatcgaaaaa
    44641 ggcgaaaaac cgttggcggc ggcgctcaag ggcgcaggtg aaatcggctt taccattatc
    44701 tcgctgacct tctcactgat tgcggtgttg atcccactgc tgtttatggg cgatatcgtc
    44761 gggcgactgt tccgcgaatt tgctattacc ctggcggtag cgattttgat ctcagcggtg
    44821 gtgtcgctga ccctgacacc gatgatgtgc gcgcggatgc tcagccagga gtcgttgcgt
    44881 aaacagaacc gcttctcccg tgcctcggaa aaaatgttcg acaggataat cgccgcctat
    44941 ggtcgtggac tggcgaaagt gctgaatcat ccgtggctga ccttaagcgt ggcactcagc
    45001 acgctgctgc ttagcgtgct gctgtgggtg ttcattccga aaggtttctt cccggtacag
    45061 gacaatggca ttattcaggg cactttgcag gcaccgcaat ccagctcctt tgccaatatg
    45121 gcccagcgac aacgccaggt cgcggacgtg attttgcagg atccggcagt gcaaagcctg
    45181 acctcatttg ttggcgttga tggcactaac ccgtcgctga acagtgcaca tttacaaatc
    45241 aacctcaaac cgttggatga acgtgatgat cgggtgcaaa aagtcatcgc ccgtctgcaa
    45301 acggcggtag ataaagtgcc gggcgtcgat ctcttcctgc aaccaacgca ggatctgact
    45361 attgatactc aggtcagccg cacccagtac cagtttacct tgcaggccac gtcactggat
    45421 gcgctcagta cctgggtgcc acagttgatg gaaaaactcc agcaactgcc acagctttct
    45481 gatgtctcca gcgactggca ggacaaaggg ctggtggcgt atgtcaatgt tgatcgcgac
    45541 agcgccagcc gtctggggat cagcatggcg gatgtcgata acgccctgta caacgcgttt
    45601 ggtcagcggc tgatttccac tatttatact caggccaacc agtatcgcgt ggtgctggag
    45661 cacaacaccg aaaatacccc aggcctcgcg gcgctggata ccattcgcct gaccagcagc
    45721 gacggcggcg tggtgccgct aagctcaatt gccaaaattg agcagcgttt tgcgccgctc
    45781 tccatcaacc atctggatca gttcccggta acgaccatct cctttaacgt gccggataac
    45841 tattcgctgg gcgatgcggt gcaggcgatt atggacaccg aaaagacgct gaatctgccg
    45901 gtggatatca ccacgcagtt ccagggcagc accctcgcct tccagtcggc gctgggcagc
    45961 actgtctggc tgattgtcgc ggcggtggtg gcgatgtata tcgtgctcgg cattctgtac
    46021 gagagcttta ttcacccgat caccattctc tcgacgctac ccaccgcagg agtcggcgca
    46081 ctgctggcgt taatgattgc cggtagcgaa ctggatgtga ttgcgattat cggcattatt
    46141 ttgctgatcg gtatcgtgaa gaagaacgcc atcatgatga tcgacttcgc gctggctgct
    46201 gagcgcgagc aaggcatgtc gccgcgcgat gccatctacc aggcttgtct gttgcgtttt
    46261 cgtccgatcc tgatgaccac tctggcggct ctgcttggcg cgctgccgct gatgttgagt
    46321 accggggtcg gcgcggaact gcgtcgtccg ttaggtatcg gcatggtcgg cggcctgatt
    46381 gtcagccagg tgctgacgct gtttaccacg ccggtgattt atctgctgtt cgaccgcctg
    46441 gcattgtgga ccaaaagtcg ctttgctcgc catgaagagg aggcgtaagt gaagtttttt
    46501 gccctcttca tttaccgtcc ggtggcgacg attttactgt cggttgccat taccctgtgc
    46561 ggcatactgg gcttccgtat gctgccggtc gccccgctgc cgcaggtcga ttttccggtg
    46621 attatggtca gcgcctcgct gcccggtgcg tcaccagaaa caatggcgtc ttccgttgcc
    46681 acgccgctgg agcgctcact tgggcgcatt gccggagtca gtgaaatgac ctccagcagt
    46741 tcgctcggca gcacgcgtat tattttgcag tttgattttg accgggatat caacggcgcg
    46801 gcgcgcgatg tgcaggcggc gatcaacgct gcacaaagtt tgctgcccag cgggatgccc
    46861 agccgtccga cctatcgcaa agctaacccg tcggatgcgc caattatgat cctcacgctg
    46921 acatccgata cttattcgca gggtgaactg tacgatttcg cctcgacgca gctggctccg
    46981 acgatttcgc aaatcgacgg tgttggtgat gtcgatgtcg gcggcagctc actgcccgcc
    47041 gtacgcgtcg ggctgaatcc gcaggcgctg tttaatcagg gcgtctcgct ggacgacgta
    47101 cgcactgcca tcagcaatgc caacgtgcgt aaaccgcagg gggcgctgga agatggcact
    47161 caccgctggc agatccagac caatgatgag ctaaaaaccg ccgccgaata tcagccgctg
    47221 attattcatt acaacaacgg cggcgcggtt cgtctgggcg atgtggcgac agtgaccgac
    47281 tctgtacagg atgtacgtaa cgccgggatg accaatgcca aaccggctat tttgctgatg
    47341 atccgcaagc tgccggaagc caatattatc cagacggttg acagcatccg ggcaaaatta
    47401 ccggagttgc aggaaaccat tccggcggcg attgatctgc aaattgctca ggatcgctcc
    47461 cccaccattc gcgcctcgct ggaagaagtc gagcaaacgc tgattatctc ggtggcgctg
    47521 gtgattctgg tggtgttttt attcctgcgc tcgggtcgcg caactattat tcccgccgtt
    47581 gcggtgccgg tttcgctgat tggtacgttt gcggcgatgt acctgtgcgg tttcagtctc
    47641 aataatcttt cgttaatggc gctcaccatc gctactggtt tcgtggtgga tgacgccatc
    47701 gtggtgctgg aaaacattgc ccgtcatctg gaagcgggaa tgaaaccgtt gcaagccgca
    47761 ctgcaaggta ctcgcgaagt cggttttacg gtgctgtcga tgagtctgtc actggtggcg
    47821 gtgttcctgc cgctgctgtt gatgggcgga ttgccgggcc gactgttacg cgaatttgcc
    47881 gtgacgcttt ctgtcgccat tggtatttcg ttgctggttt ctctgacatt aacgccaatg
    47941 atgtgtggct ggatgctgaa agccagcaag ccgcgcgagc aaaagcgact gcgtggtttt
    48001 ggtcgcatgt tggtagccct gcaacaaggc tacggcaagt cactgaaatg ggtgctcaat
    48061 catacccgtc tggtgggcgt ggtgctgctt ggcaccattg cgctgaatat ctggctgtat
    48121 atctcgatcc cgaaaacctt cttcccggag caggacactg gcgtgttgat gggcgggatt
    48181 caggcggatc agagtatttc gtttcaggcg atgcgcggta agttgcagga tttcatgaaa
    48241 attatccgtg acgatccggc agtggataat gtcaccggct ttacaggcgg ttcgcgagtg
    48301 aacagcggga tgatgtttat caccctcaag ccacgcgacg aacgcagcga aacggcgcag
    48361 caaattatcg accgtctgcg cgtaaaactg gcgaaagaac cgggggcgaa tctgttcctg
    48421 atggcggtac aggatattcg cgttggtggg cgtcagtcga acgccagcta ccagtacacg
    48481 ttgttatccg acgacctggc ggcactgcgc gaatgggagc cgaaaatccg caaaaaactg
    48541 gcgacgttgc cggaactggc ggacgtgaac tccgatcagc aggataacgg cgcggagatg
    48601 aatctggttt acgaccgcga caccatggca cggctgggaa tcgacgtaca agccgccaac
    48661 agtctgttaa ataacgcctt cggtcagcgg caaatctcga ccatttacca gccgatgaac
    48721 cagtataaag tggtgatgga agtggatccg cgctataccc aggacatcag tgcgctggaa
    48781 aaaatgttcg ttatcaataa cgaaggcaaa gcgatcccgc tgtcgtattt cgctaaatgg
    48841 caaccggcga atgccccact atcggtgaat catcagggat tatcggcggc ctcgaccatt
    48901 tcgtttaacc tgccgaccgg aaaatcgctc tcggacgcca gtgcggcgat cgatcgcgca
    48961 atgacccagc ttggggtgcc ttcgacggtg cgcggcagtt ttgccggcac ggcgcaggtg
    49021 ttccaggaga cgatgaactc gcaggtgatc ctgattatcg ccgccatcgc cacggtgtat
    49081 atcgtgctgg gtatccttta cgagagttac gtacatccgc tgacgattct ctccaccctg
    49141 ccctcggcgg gcgttggagc gctgttggcg ctggagctgt tcaatgcccc gttcagccta
    49201 atcgccctga tagggatcat gctattaatc ggcatcgtga agaaaaacgc cattatgatg
    49261 gtcgattttg cgcttgaagc ccaacggcac ggtaacctga cgccgcagga agctattttc
    49321 caggcctgtc tgctgcgttt tcgcccgatt atgatgacta ccctggcggc gctgtttggt
    49381 gcgctgccgc tggtattgtc gggcggcgac ggctcggagc tgcggcaacc cctggggatc
    49441 accattgtcg gcggactggt aatgagccag ctccttacgc tgtataccac gccggtggtg
    49501 tatctctttt tcgaccgtct gcggctgcgt ttttcgcgta aacctaaaca aacggtaacc
    49561 gagtaaatga cagatcttcc cgacagcacc cgctggcaat tgtggattgt ggctttcggc
    49621 ttctttatgc agtcgctgga caccaccatc gtaaacaccg cccttccctc aatggcgcaa
    49681 agcctcgggg aaagtccgtt gcatatgcac atggtcattg tctcttatgt gctgaccgtg
    49741 gcggtgatgc tgcccgccag cggctggctg gcggacaaag tcggcgtgcg caatattttc
    49801 tttaccgcca tcgtgctgtt tactctcggt tcactgtttt gcgcgctttc cggcacgctg
    49861 aacgaactgt tgctggcacg cgcgttacag ggcgttggcg gcgcgatgat ggtgccggtc
    49921 ggcagattga cggtgatgaa aatcgtaccg cgcgagcaat atatggcggc gatgaccttt
    49981 gtcacgttac ccggtcaggt cggtccgctg ctcggtccgg cgctcggcgg tctgctggtg
    50041 gagtacgcgt cgtggcactg gatctttttg atcaacattc cggtggggat tatcggtgcg
    50101 atcgccacat tgatgttaat gccgaactat accatgcaga cgcggcgctt cgatctctcc
    50161 ggctttttat tgctggcggt tggcatggcg gtattaaccc tggcgctgga cggcagtaaa
    50221 ggtacaggtt tatcgccgct gacgattgct ggcctggtcg cagttggcgt ggtagcactg
    50281 gtgctttatt tgctgcacgc cagaaataac aaccgcgctc ttttcagtct gaaactgttc
    50341 cgcactcgta ccttttcgct gggcctggcg gggagctttg ccggacgtat tggcagtggc
    50401 atgttgccct ttatgacacc ggttttcctg caaattggcc tcggtttctc gccgtttcat
    50461 gccggactga tgatgatccc gatggtgctt ggcagcatgg gaatgaagcg aattgttgta
    50521 caggtggtga attgctttgg ttatcgtcgg gtactggtag cgaccacgct gggtctgtcg
    50581 ctggtcaccc tgttgtttat gactaccgct ctgctgggct ggtactacgt tttgccgttc
    50641 gtcctgtttt tacaagggat ggtcaactcg acgcgtttct cctccatgaa caccctgacg
    50701 ctgaaagatc tcccggacaa tctggcgagc agcggaaaca gcctgctgtc gatgattatg
    50761 caattgtcga tgagtatcgg cgtcaccatc gccgggctgt tgctgggact ttttggttcg
    50821 cagcatgtca gcgtcgacag cggcaccaca caaaccgtct ttatgtacac ctggcttagc
    50881 atggcgttga tcatcgccct tccggcgttc atctttgcca gagtgccgaa cgatacgcat
    50941 caaaatgtag ctatttcgcg gcgaaaaagg agcgcgcaat gaagttctgg cgacccggta
    51001 ttaccggcaa actgtttctg gcgattttcg ccacctgcat tgtcttgctg atcagtatgc
    51061 actgggcggt gcgtatcagt tttgagcgtg gctttattga ttacatcaag catggtaatg
    51121 agcagcggct gcaaatgctc ggcgatgccc tgggcgagca gtatgcgcag catggcaact
    51181 ggcgcttcct gcgcaacaat gatcgctttg tctttcagat cctgcgttca tttgaacacg
    51241 ataattcgga agataaaccc ggcccgggta tgccaccgca cggctggcgt acccagttct
    51301 gggtggttga tcaaaacaac aaagtgctgg ttggtccgcg agcgccgatt ccacctgacg
    51361 gtacacggcg acccattctg gtcaacggtg cggaagttgg cgcggtgatc gcctcccccg
    51421 ttgagcggtt aacgcgcaat actgatatca atttcgataa acaacagcgg caaaccagct
    51481 ggttgattgt cgctctggca acgttactcg cggcgctcgc cacctttctg ctggcgcgcg
    51541 gtttactggc accggtaaaa cgacttgtcg atggcacgca caaactggcg gcgggcgatt
    51601 tcactacccg cgtaacgccc accagtgaag atgaactggg caaactggcg caagacttca
    51661 accagcttgc cagcacactg gagaaaaacc agcaaatgcg gcgcgatttt atggccgata
    51721 tttctcacga actgcgtaca ccattagcgg tgctgcgcgg tgaactggaa gccattcagg
    51781 atggcgtgcg taaattcacg ccggagacgg tggcgtcttt acaggcggag gtcggtacac
    51841 tgaccaaact ggttgacgat ctccatcagt tgtcgatgtc tgatgaaggc gctctcgcct
    51901 atcaaaaagc accggtagat ttgatcccac tgctggaagt ggcgggcggc gcatttcgcg
    51961 aacgattcgc cagtcgtggc ctgaaactgc aattttccct gccagacagt attaccgtat
    52021 ttggcgatcg cgaccgttta atgcagttat tcaataactt actggaaaac agcctgcgct
    52081 acactgacag cggcggcagc ctgcaaatct ctgccgggca gcgcgacaaa acggtgcgcc
    52141 tgacctttgc cgacagtgcg ccaggtgtca gtgacgatca gctacaaaaa ttgtttgaac
    52201 gtttttatcg caccgaaggt tcccgcaacc gtgccagcgg cggttccggg ctggggctgg
    52261 cgatttgcct gaacattgtt gaagcacata atggtcgcat tattgctgcc cattcgcctt
    52321 ttggcggggt aagcattaca gtagagttac cgctggaacg ggatttacag agagaagtat
    52381 gaccgagtta ccaatcgacg aaaacacacc gcgtattttg atcgtggaag atgaaccgaa
    52441 gctggggcag ttgctcattg attatctgcg tgctgcgagc tatgcgccga cgcttatcag
    52501 ccacggcgat caggtactgc cgtatgtgcg ccagacacca ccggatctga tcctgttaga
    52561 tctgatgctc cctggcaccg atggcctgac gctgtgccgg gaaattcgtc gtttttctga
    52621 cattccgatc gtgatggtga cggcaaaaat cgaagagatc gatcgcctgc tggggctgga
    52681 gattggcgca gatgattata tctgtaagcc gtacagccca cgggaagtgg tagcgcgcgt
    52741 caaaaccatt ttgcgccgtt gcaaaccgca gcgcgaattg cagcaacagg atgctgaaag
    52801 cccgttgatt atcgacgaag gtcgttttca ggcttcatgg cgcggtaaaa tgcttgacct
    52861 gacgcctgcg gaatttcgtc tgctgaaaac gctatctcat gaaccaggaa aagtgttctc
    52921 ccgcgagcat ttgctcaatc atctttatga cgactaccgc gtagtaaccg accgcaccat
    52981 cgacagccac attaaaaacc tgcgccgcaa gctggaatct ctcgacgccg aacagtcatt
    53041 tatccgcgcc gtttacggcg tcggttaccg ctgggaagcc gacgcctgcc gcatcgttta
    53101 gttttagcga cattattttg ttagccggag atggcgttca gacccgatcc ggcaacatta
    53161 tcccacgcat ggtcagcaaa ctgacattat ctctccgccc tggcaggcat cagaaaatcg
    53221 ctcatacttt aatcggtaaa cagcaccttt agatgctgtt ttcgatacac aatttcaatc
    53281 aaggagtcat tatggctggt tggtttgaac tcattaagag cagtgataat cagttccggt
    53341 ttgtgctaaa agcgggcaat ggtgagacta tcctcaccag cgagctgtat acctcaaaag
    53401 cctctgcgga aaagggcatc gcgtcggtgc gtagcaacag cccgcaagaa gaacgctatg
    53461 agaaaaaaac ggcaagtaac ggcaaattct atttcaatct gaaagccgct aatcatcaaa
    53521 ttatcggctc cagccagatg tacgccaccg cgcaatctcg tgaaaccgga attgcctccg
    53581 ttaaagtcaa tggcacaagc cagacggtga aagacaatac gtaattacga tgccgggcgt
    53641 gggttgttgg cgtccggctt tatatccaaa taacgcttag ttattaataa ttaagagtaa
    53701 ccgccgccat taaaatagtg ggcactgacg agcactcgtt gtgctttctt tgtttaccat
    53761 cccatactta tcctcctgaa catgttcatg tgccaaaata tcttctaata ctttcgtgac
    53821 gctatccggt ttatgacata cactgatgta agatttttac ttacaggcgg gcgtaaaatg
    53881 gccagaacga tgacggttga tctcggcgat gagttgcgtg aatttatcga gtccttaatt
    53941 gaatccggcg actatcgcac ccaaagcgaa gtgatccgtg agtcactgcg attacttcga
    54001 gaaaagcaag cagagtctcg cctgcaagcg ttacgtgaca tgctcgcaga agggttgagt
    54061 agtggtgaag ctcagccgtg ggaaaaggac gcgttcttac ggaaggtgaa agcagggata
    54121 aggaaataat gtgcgtatta ttaaactcat gccaaaagcc aatgaggatt tagaaggcat
    54181 ctggtattac agctatcacc atttcggcga accacaagcc gacagatatg tggaacatct
    54241 ttcagacgtt ctccagattc tgagtaacaa taatataggt acgcccagac ctgaattagg
    54301 cgaaggtatt tttgttcttc cttttgaacg ccacgtcatt tatttcctgc aatcaccagg
    54361 ggaaattatc gtcattcgta tccttaacca aaaccaggac gccaccagac atttacactg
    54421 gagctaaacc cgccgattgc gctacaatgc ccgcccttaa agtgggggca ctcccctaac
    54481 cgcttcatca ggtgaagcgg atctgacctg tcatcagaac gagagaatta tgtttaaacc
    54541 ggaactcctt tccccggcgg gaacgctgaa aaatatgcgt tacgctttcg cttatggcgc
    54601 agatgctgtt tatgcgggcc agccgcgtta ttccctgcgt gtgcgcaaca acgaattcaa
    54661 ccacgaaaat cttcagctcg gcatcaatga agcccacgcg ctggggaaaa agttttatgt
    54721 cgtggtcaac attgcaccgc acaacgccaa gctgaaaacc tttatccgtg acctgaaacc
    54781 ggtggtggaa atggggccgg atgcgctgat tatgtccgat ccagggctga ttatgctggt
    54841 gcgtgagcac ttccctgaaa tgccgatcca cctttcggtg caggctaacg ccgtgaactg
    54901 ggcgacggtg aaattctggc agcaaatggg cctgacccgc gtgatcctct ctcgcgagct
    54961 gtcgctggaa gagattgaag agatccgcaa tcaggtgccg gatatggaga tcgagatctt
    55021 cgttcacggc gcgctgtgca tggcctactc cggtcgctgc ctgctctctg gctatatcaa
    55081 caagcgcgac ccgaaccagg gcacctgcac caacgcctgc cgctgggagt acaacgtcca
    55141 ggaagggaaa gaagatgatg ttggcaacat cgtacacaag tacgagccga ttccggtgca
    55201 aaatgttgag ccgacgctgg gtatcggcgc accaaccgac aaagtgttta tgatcgaaga
    55261 ggcccagcgt ccgggcgagt atatgaccgc gtttgaagat gagcacggca cttacatcat
    55321 gaactcgaaa gatctgcgcg ccatcgccca tgtagaacgc ctgaccaaaa tgggcgtgca
    55381 ttcgctgaaa atcgaaggtc gtaccaaatc tttctactat tgtgcacgca ccgcacaggt
    55441 ttaccgcaaa gctatcgatg acgccgctgc gggaaaaccg ttcgatacca gcctgctgga
    55501 aactctggaa ggtctggcgc atcgtggcta taccgaaggt ttcctgcgtc gtcatactca
    55561 cgacgattat cagaactacg aatacggtta ttcagtttct gaccgccagc agtttgttgg
    55621 tgagtttacc ggtgagcgca agggggacct cgcggcggta gcggtgaaaa ataaattctc
    55681 cgttggcgac agccttgagc tgatgacgcc gcaaggcaac attaatttta cccttgagca
    55741 catggaaaac gccaaaggtg aagctatgcc gatagcacca ggcgatggtt atactgtgtg
    55801 gctcccggtc ccgcaggatc ttgagctcaa ttacgcgctg ctgatgcgta atttctccgg
    55861 ggaaaccacg cgtaatcccc acggtaagtg attaatttcg attatttttc ccggatggaa
    55921 aattcttaga aaccgatcac atacagctgc atttattaag gttatcatcc gtttcgctga
    55981 aaaacataac ccataaaatg ctagctgtac caggaaccac ctccttagcc tgtgtaatct
    56041 cccttacacg ggcttatttt ttatgcataa gccctatccc tggtcaccgt cttccattga
    56101 ccacatcgat agaatctccc ttcatagcac gatgcctttc acgtaacggc atcgtgctcg
    56161 cacaggttcc ggctaagcac aaccagaacg cgcatgtttg acgcttacca aaaaatattc
    56221 tcactctcca catttgaatg tcagacgagc gacgccatgt aatcctgcac cttctgtctt
    56281 caggtcaact atctgcattt ttttgccctg agtaacacag aaatgggctg catcattttt
    56341 tactatattt tctgcaccag atattctgcc cctggctaaa gaagcttcgg cttcggtgta
    56401 gtattggtta tcgagtttac gctgaatatt acttttatat gcaagaccaa atttaccgat
    56461 acttgtctca tcattatgca cagcacaacc agacacaata aaaatactaa ttaatgatat
    56521 agcagctatc tttttcatct caccttcccc cattaaatac caacgacact ctctagtgtt
    56581 taaatataat aatggcatga ttattataat tgaataggat tataataaat gttctgtaca
    56641 acatttccta cataagtagg aattacggac attgaggccc ttcagggtaa ctccatgggg
    56701 gctttaatat attatattga agatgccact gtttagttga atattaggta tatgctcttt
    56761 tttgaaattt atcggtggca gccgttagta ttcgctgtcc ccattgcaag ctcctggtgg
    56821 taaccactga atcctccata cttgaactga ctttttatcc tccgactttc atcctgttct
    56881 gactccacct tttgttttct gctctacact atctacagac caatcataaa ggcacatacg
    56941 atcatggcag aatttcccgc cagcttactg attcttaatg gcaaaagtac tgacaatcta
    57001 cccttgcgcg aagcaattat gctgttgcgt gaggaaggaa tgacgatcca tgtgcgggtc
    57061 acctgggaga aaggcgatgc cgcacgatat gtagaggagg cacggaagtt gggcgtcgca
    57121 acggtgattg ccggtggtgg cgatggcacc attaatgaag tttctacggc gttgattcag
    57181 tgtgaggggg atgacatacc cgcgctggga attttgccat taggaaccgc caatgatttt
    57241 gccaccagtg tagggattcc tgaggcactg gataaggcgc tgaaactggc aattgccggt
    57301 aacgccattg cgatagatat ggcgcaggtc aacaaacaaa cctgttttat taatatggcg
    57361 acaggcggat ttgggacgcg tattaccaca gaaacgccgg aaaaattaaa agccgcgctg
    57421 ggtggcgtct cttacatcat tcatggctta atgcgcatgg atactctgca accggaccgt
    57481 tgtgaaatcc gcggtgaaaa ctttcactgg caaggtgacg ccctggtcat tggtattggt
    57541 aacgggcgtc aggccggtgg cggtcaacaa ttgtgcccga acgcgttaat taacgatggc
    57601 ttgctgcaac tgcgcatttt taccggcgat gaaattcttc cggctctcgt atcaacctta
    57661 aaatctgacg aagataaccc gaatattatc gaaggcgctt cgtcgtggtt tgatatacaa
    57721 gccccacacg aaatcacttt taatcttgat ggcgaaccgt tgagtgggca aaacttccat
    57781 attgaaatac ttccggcggc gttgcgttgt cgattaccac cggattgtcc attattgcgt
    57841 taatcaaact attctctctt gaaccaggga gaatccgaaa tgccggatgc ggtgtaaata
    57901 ccttatccgg catacagaac aatactacac cgtaattaat tttacccgtg tcttctgcaa
    57961 tgccagttta tccacctctg atacagcatc atcggtaata ataatgtcaa ttttttcgat
    58021 tggcagtacc tgattaaaac cgcgacggtt aaatttcgat gaatcgagaa cggcaatcac
    58081 tttattagcg gcggtgacca tcgcaccgct aatcgaataa ccttcattaa aggtcgtaat
    58141 accattaacc gcatcaatac catcagcacc gacaaacatt aaatcggcat taatatcctg
    58201 cagggaacgt tcagcaatag atccatgcat agatcgcgtt ttatggcgga ccgtgccacc
    58261 gcagacgacc agagtaatgt ctttattttc ggaaagggca aacgcagccg ggagactgtt
    58321 ggtaatcacc gtgatatttt tagcggtcat taatccttcg gcgataagca ttgtggtact
    58381 tccgctgtcg agaataacgg tcatcccttc atggatcatt ttgactgccg cctgcgctat
    58441 tctgtttttg ggcgcgctgg cgagctgaaa tcgctcttta aacccgactt cctgggtctc
    58501 ggtttcacta ttaccggaca ttattttcgc cgcaccgcca tgaaagcgcg taaccacgcc
    58561 tttttgttcg agaaagcgca aatcggcacg gattgtcgct tccgaggcag caaatactcc
    58621 cgccagatcc tgaacaagca cggttccctg ttcattcact aattggatga tcttattcct
    58681 tcgctcgaat gaattcatcg tcatttaccc atattcaatt gtggctagtg taaacgaagg
    58741 gggaccattg gtgaatgatc tgttttgcac aataccgggc cagcatcacg ctggcccgcg
    58801 gtttcatgga atgagcaaca ctttgcccgg catggcatta cgagcgatgt cacgcaccgc
    58861 ctgggtgaag ctttcaaagc ttccacggtg agcgattaat ggctccaggc ttaacttacg
    58921 ttctgtcagc aaccggctcg ccgtttccca ctcctgcccc ggccaagggc tggaatagtt
    58981 catccaactg ccgataaccg tcagctcttt acgcaatatt ttgccaaacg ttgccgatgt
    59041 taaatgcaga tcctgatgca acgtgcccac cagcgccagt tgggcatgag gaccggcaat
    59101 ctctaccgcc agttcgacag tttgcggtac gccagccgtc tcgaggataa gctgattaaa
    59161 acgcagttcg cgtaaaacgc tctgcatttg cggcgcgctc atttcgctac tgttaaatgt
    59221 ttgcatcgca ccgaaagatt ttgccagtgc cagtttttct gagctaatgt cgatagccgt
    59281 cacactcttt gctcccagcg cgacagcgca ctgaatagcc agcaggccaa tggttccggc
    59341 accaataata ataacgtttt tattctcaca accttgcgct aaatgaaaag catgcagacc
    59401 aacggtaatc ggctcaataa aagccccatc ctcaataggc atatccgtgg gtagagcaaa
    59461 gacatttttt cgcttaacga caatatattc agcaaatcca ccatcacgcc gcgagccaat
    59521 aaaatcatat tttgcgcact gggaataaaa ccctttcaga cactctggac aagtaaaaca
    59581 gggtaataac ggcacacagg caaccgcatc gccagggtgt aaatcatcaa caccggatcc
    59641 caccgcatca atatagccgc taaattcatg gcctaacgtt attggataat aatgtgcacc
    59701 atttttaaat atcctgggta aatcggaacc acataagccc gagctggcaa tttttacccg
    59761 cacctcatcc tgatgtttaa tttcaggaat gacgctttct gcaacgcgca cgataccatc
    59821 agtatcattc accactgatt tcataaaaac tcctgattgt taagggggat aacctccccc
    59881 tcaggtaaaa attattctgc gagaacgact ttctcttgtt taataaagcc acgcgctcta
    59941 cgccaggtca tgaaaatacc ggtcagataa attgcaccga taataatgaa accgggaata
    60001 ttttgcgggg agaaaacctg aatcagtaac caggtaatgg gagaaccgcc ctgatccatt
    60061 gaagccacca tacccccggc ttttaacgcc ccagcattag ccgccagttg ggtgtgcaaa
    60121 ccaatagttt gcgtcgcgat ccacagggtg atgctcataa tgatgacacc cgagattaag
    60181 gtgcggaaca gatttccacg atgcaccgcg accgccatcg ccacaaagaa gccgatagtg
    60241 gcaagatcgc caaacggcag cacctgatta cccggcacac aaacagcaat taaaatggtg
    60301 agtgggataa aaatcaggct tgccgatacc accgccgtat gccccagcag taatgcggga
    60361 tccaggccaa tcaggaactc ctgaccgccg aacttcgcct gtaaacgact acgggcctgc
    60421 ttagcgatgg gcgttaaacc atccatgatg ggtttaatca cccgtggcat cagcagcatc
    60481 actgccgccg tttttaccgc cagctgcaat acacctttga catcgtaacc cgcgaggatg
    60541 ccgataatca gccccatcac aaaacccacg gtgacaggtt cgccaaaagg gccaaatttg
    60601 cgctgaatat cgtcggcgct aaatttaatt cggttaacgc ctgggatttt ctcgatgata
    60661 gcatcgacca gcaccgcaat cggccccata tacgccgacg taccgtgcgg aatagcaata
    60721 ccttccagct caaagaaatt tcgggtatcg cgggcaaacc agtcgccgag cttataaaca
    60781 aacgccgcgt gaattaccac acctgccatc cctatcatcc atgaaccggt tgccaggtgc
    60841 agcaacgcgc cggtgaaggt catatgccag atattccaga tatcaacatt taccacccgc
    60901 gtcatacggg tcagtaacat cgccacgtta accaatatcg caatcggaat cgccaccagc
    60961 gcaatttgcg acgcccaggt cattggtgaa gagcccggcc agccgacatc gaccacatgc
    61021 agattcaggt cgaaattttc cgccatcgct ttcgccgccg gaccaatgga atccagcatt
    61081 aagccaatca caaggccaat gccaacaaag ccaatcccga tatgcagacc cgctttaaag
    61141 caatcgcctg ccttcatgcc taatatttta gaaaaaataa tgatgacaac cggcagcatc
    61201 actgtagggc cgaggtcgag aatataacgc atgacttctg aaaacatagg tcacccctgt
    61261 aagatagtca gaattttatt ttgtaatgct tcgataccga caccagaaat aaaaggcatg
    61321 ccgtgaacta acggaatatc gccaaaacta cgatccactt tggcagtggt gcatatcaaa
    61381 tgcacaccat ccatataggt ttctatttca ttaacccgac actggattaa ttcaacagga
    61441 atattatgat tctgacacaa ctctttaatt tcttccgccg ccatcgtaga ggtcgcaacc
    61501 gcgcctccgc aagcgacaat aatcttgcgt ttcataaata gtccttttta ttgttacaga
    61561 gggatttatt cgtctgaatc taaaacatac tttgtgaagt attcctttaa ctgggtttca
    61621 ggaagagtga ttagtgtctc gacgatatcg ggctgttgta acttgccaaa taaacagcgt
    61681 aaaagtttca attgctgctg cggattttcc acaatcaacg caataaccaa cgatacctcc
    61741 acgtcgttat catcatccgc ttgctgaaaa tgaactttat ttgttggcct taacagataa
    61801 atggctgacg acttagcatg aatcgcctca caatgcggta ttgcaatggc gtgctgctca
    61861 agcattatcc cggtagggaa ttctgcttct ctggcaatta atgcctgtgg ccaggtatca
    61921 tgaaccacac ctttggcgag catctcatta ccgatatggg ttaaaacttc gctacgatcg
    61981 acaaaagaaa ttccgctacg aacaaacagg ttagtcatat accgtcctta ttccgcacag
    62041 ccgtagcgat aggcgcgcaa aacatcataa attttatcca taatcagctg atgcggtatt
    62101 gctgataatt ccccggactg aatgcgttca aattgtttgg gaagatactg actaatcatg
    62161 cccagtgggg tgtccacgcc ttcaagattc accatcatcg tttcgacgct atttttaatc
    62221 cgactatgcg gccagtaata acgaatacga tccgacaggc tgtaacgaat atccagtaat
    62281 gaatcgttaa aacccgtacg ataatatttt ttccagtatt gcggttcgtc gagcatcact
    62341 tcttcaatta ccgccaggca accgctgcga ttttcggggg cgataagttc ctgctcaatt
    62401 tgtgccagtg caaatatcgc ctcgcgtaaa gcaaaggtta atgcgggacc gactttcaat
    62461 attgcaaagt gatcgcggac taattcccaa taagccgtcc gggtctggta atcggtagaa
    62521 tgtgcttcat aaaccattcg ggtgttttct atccattgcg ccagcgcctg cgcttcctgc
    62581 ggctgataat ggataatatt gctgtgatca aattccacac ccggctgcac cacgatggca
    62641 atcacacgtg ttaacgcctc tgtcagccca cgggcaataa aggccttttg atgcgtacgt
    62701 aaagtattgg cggcatcttc aacatgggtg atgtgtactg actgaatggc gctggcctca
    62761 ccgcccggaa ccggaacttc ggtgccaatg acatagctca gttgctcacg ctggcaatct
    62821 gtcgccacac tttccgcagc aaagcaaagc acagcagctc gttccgcaac cgtttctggt
    62881 gctaacggta tgggatcccc cgcgcaggac attgacgcat caagatgaat tttactgaag
    62941 ccagcacgaa catatgcttt taccagctcg acggattttt ccatcgccgc atccgcattt
    63001 tcttgctgcc agcagtttgg ccccagatga tcgccgccga gaataatgcg ttcgcgtgca
    63061 aacccaactt tatcggcaat cgtaaaaaca aattcgcgaa agtctgccgg tgtcattccg
    63121 gtataaccgc caaattgatt gacctggttt gacgttgctt caatcagcac tttgcgcgtg
    63181 ctgttgcgat caaatgccag cgccgcttcg ataaccaacg gatgggcaga acagactgaa
    63241 catatgccga tatgttcacc agctttatgc cgggcaatta acgttttcat gttttttcct
    63301 tgttaaatgg caggtgcgtt atgccctgcc ctcgcagcca caatcggcaa tcactttgct
    63361 caccacatcg cgcattgcgg atttagccga ctgcaaataa tcccggggat cggtcgcttc
    63421 agggtgctcg gtcaggtaat tttttaacgc ctgcgagaag gcatttttca gctccgttgc
    63481 aacgttgatt ttgcatatcc ccagtttgat ggtttgctga atatccttag tcgataaccc
    63541 tgacgcgcca tgcagcacca gcggtaagtt cacccactgg cgaatgttct ccagtcgaga
    63601 aaaatcaagc gccggtgcgc tggcatacat cccatgagcc gtgccgatgg cgaccgccag
    63661 ggaatcaatt ccggttgcct cggcaaattc acgcgcctga gcggggttgg tataaaaagc
    63721 atcggcttca ttgacttgca catcatattc ctggccgcca agttgcccca gctccgcttc
    63781 gacgctgaca tcaaagcgat ggcaaaaatc caccacctct ttgacccgtg aaatattttg
    63841 cgcaaaaggc aaatgcgagg cgtcaatcat gactgagcgc acgccagaac gaaccttctg
    63901 agcgatatcg tcaaatttcg tgtgatggtc gagatgaatt gccagtggat ggtgatattg
    63961 cttcgccatc gcgctgacca gcgccaacag attttctgta ccagcatgag taaatgtgcc
    64021 aggcgttccg gcgatgatga ccggcgcatg caggttggca gcggtttcca ccaccacttg
    64081 catcgtttcg agattgtgaa tattgaatgc cggaaccgca taaccgccgc gctgtgcgtt
    64141 gttcagcatc tgctttgtcg ataccacgta
//
LOCUS       DAZFQD010000013        53908 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.013, whole genome shotgun sequence.
ACCESSION   DAZFQD010000013 DAZFQD010000000
VERSION     DAZFQD010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 53908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53908
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.013"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            166..1455
                     /gene="sdaC"
                     /locus_tag="ACHKTW_000898"
     CDS             166..1455
                     /gene="sdaC"
                     /locus_tag="ACHKTW_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HFO6461967.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
     gene            1513..2880
                     /gene="sdaB"
                     /locus_tag="ACHKTW_000899"
     CDS             1513..2880
                     /gene="sdaB"
                     /locus_tag="ACHKTW_000899"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HFO6461968.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     NSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            2992..3747
                     /gene="xni"
                     /locus_tag="ACHKTW_000900"
     CDS             2992..3747
                     /gene="xni"
                     /locus_tag="ACHKTW_000900"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0017108 - 5'-flap endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0033567 - DNA replication, Okazaki fragment
                     processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HFO6461969.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKAGRPPMPEELHDEMPALRAAFEQRGVPCWSTSGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(3802..4950)
                     /gene="fucO"
                     /locus_tag="ACHKTW_000901"
     CDS             complement(3802..4950)
                     /gene="fucO"
                     /locus_tag="ACHKTW_000901"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HFO6461970.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEEARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDIPALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(4978..5625)
                     /gene="fucA"
                     /locus_tag="ACHKTW_000902"
     CDS             complement(4978..5625)
                     /gene="fucA"
                     /locus_tag="ACHKTW_000902"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008738 - L-fuculose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006004 - fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HFO6461971.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRSIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVAQALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            6172..7488
                     /gene="fucP"
                     /locus_tag="ACHKTW_000903"
     CDS             6172..7488
                     /gene="fucP"
                     /locus_tag="ACHKTW_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015150 - fucose transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015756 - fucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HFO6461972.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSGHFRLNLAQT
                     FNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEIPGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFARFRSQTATN"
     gene            7521..9296
                     /gene="fucI"
                     /locus_tag="ACHKTW_000904"
     CDS             7521..9296
                     /gene="fucI"
                     /locus_tag="ACHKTW_000904"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008736 - L-fucose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019317 - fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HFO6461973.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALAWADKNFRYGEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVREPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGNPTMKPHWEISQQEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            9405..10823
                     /gene="fucK"
                     /locus_tag="ACHKTW_000905"
     CDS             9405..10823
                     /gene="fucK"
                     /locus_tag="ACHKTW_000905"
                     /EC_number="2.7.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008737 - L-fuculokinase activity
                     [Evidence IEA];
                     GO_process: GO:0042355 - L-fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HFO6461974.1"
                     /translation="MKQEVILVLDCGATNVRAIAVNRQGKIVARASTPNASDIAMENN
                     TWHQWSLDAILQRFADCCRQINSELTECHIRGIAVTTFGVDGALVDKQGNLLYPIISW
                     KCPRTAAVMDNIERLISAQRLQAISGVGAFSFNTLYKLVWLKENHPQLLERAHAWLFI
                     SSLINHRLTGEFTTDITMAGTSQMLDIQQRDFSPQILQATGIPRRLFPRLVEAGEQIG
                     TLQNSAAAMLGLPVGIPVISAGHDTQFALFGAGADQNEPVLSSGTWEILMVRSAQVDT
                     SLLSQYAGSTCELDSQAGLYNPGMQWLASGVLEWVRKLFWTAETPWQMLIEEARLIAP
                     GADGVKMQCDLLSCQNAGWQGVTLNTTRGHFYRAALEGLTAQLQRNLQMLEKIGHFKA
                     SELLLVGGGSRNTLWNQIKANMLDIPVKVLDDAETTVAGAALFGWYGVGEFNSPEEAR
                     AQIHYQYRYFYPQTEPEFIEEV"
     gene            10825..11247
                     /gene="fucU"
                     /locus_tag="ACHKTW_000906"
     CDS             10825..11247
                     /gene="fucU"
                     /locus_tag="ACHKTW_000906"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016854 - racemase and epimerase activity
                     [Evidence IEA];
                     GO_function: GO:0042806 - fucose binding [Evidence IEA];
                     GO_process: GO:0042354 - L-fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HFO6461975.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMGPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            11305..12036
                     /gene="fucR"
                     /locus_tag="ACHKTW_000907"
     CDS             11305..12036
                     /gene="fucR"
                     /locus_tag="ACHKTW_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HFO6461976.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTA"
     gene            complement(12080..13180)
                     /gene="rlmM"
                     /locus_tag="ACHKTW_000908"
     CDS             complement(12080..13180)
                     /gene="rlmM"
                     /locus_tag="ACHKTW_000908"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HFO6461977.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPADAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(13173..13568)
                     /gene="ygdD"
                     /locus_tag="ACHKTW_000909"
     CDS             complement(13173..13568)
                     /gene="ygdD"
                     /locus_tag="ACHKTW_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HFO6461978.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            complement(13587..14504)
                     /gene="gcvA"
                     /locus_tag="ACHKTW_000910"
     CDS             complement(13587..14504)
                     /gene="gcvA"
                     /locus_tag="ACHKTW_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HFO6461979.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGLNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            complement(14855..15082)
                     /gene="ygdI"
                     /locus_tag="ACHKTW_000911"
     CDS             complement(14855..15082)
                     /gene="ygdI"
                     /locus_tag="ACHKTW_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HFO6461980.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDTGMISYKDANGNKQQINRTDVKEMVELDQ"
     gene            15274..16479
                     /gene="csdA"
                     /locus_tag="ACHKTW_000912"
     CDS             15274..16479
                     /gene="csdA"
                     /locus_tag="ACHKTW_000912"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HFO6461981.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTGAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGAVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMVHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAESWSRSLATLAEDALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            16479..16922
                     /gene="csdE"
                     /locus_tag="ACHKTW_000913"
     CDS             16479..16922
                     /gene="csdE"
                     /locus_tag="ACHKTW_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HFO6461982.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLSQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVTENGKMHFFGDSEGRIVRGLLAVLLTAVEGK
                     TVAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAAAKQV"
     gene            complement(16973..17779)
                     /gene="tcdA"
                     /locus_tag="ACHKTW_000914"
     CDS             complement(16973..17779)
                     /gene="tcdA"
                     /locus_tag="ACHKTW_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HFO6461983.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSVGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PSQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(17856..18953)
                     /gene="mltA"
                     /locus_tag="ACHKTW_000915"
     CDS             complement(17856..18953)
                     /gene="mltA"
                     /locus_tag="ACHKTW_000915"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HFO6461984.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEVYAGA
                     LSDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            19162..19238
                     /locus_tag="ACHKTW_000916"
     tRNA            19162..19238
                     /locus_tag="ACHKTW_000916"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19196..19198,aa:Met,seq:cat)
     gene            complement(19313..20566)
                     /gene="amiC"
                     /locus_tag="ACHKTW_000917"
     CDS             complement(19313..20566)
                     /gene="amiC"
                     /locus_tag="ACHKTW_000917"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Like AmiA, dependent on TAT (Twin-Arginine
                     Translocation) system for export; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HFO6461985.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEKEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            20798..22129
                     /gene="argA"
                     /locus_tag="ACHKTW_000918"
     CDS             20798..22129
                     /gene="argA"
                     /locus_tag="ACHKTW_000918"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HFO6461986.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     IHRQLDSGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERITAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(22191..24017)
                     /gene="recD"
                     /locus_tag="ACHKTW_000919"
     CDS             complement(22191..24017)
                     /gene="recD"
                     /locus_tag="ACHKTW_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HFO6461987.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASHPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHAIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERAGQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE"
     gene            complement(24017..27559)
                     /gene="recB"
                     /locus_tag="ACHKTW_000920"
     CDS             complement(24017..27559)
                     /gene="recB"
                     /locus_tag="ACHKTW_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HFO6461988.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLTVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYERLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIALVVFETWKGPQALLRDINRYLQGEAPVIKAPPPDDETL
                     ASRHAQIVARIDTVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIEKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIEQLLAEPLSIRDLVITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDAFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDVVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEVERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYDRHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIALMDEMFAGMTLEEA"
     gene            complement(27552..30440)
                     /gene="ptrA"
                     /locus_tag="ACHKTW_000921"
     CDS             complement(27552..30440)
                     /gene="ptrA"
                     /locus_tag="ACHKTW_000921"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HFO6461989.1"
                     /translation="MPRSTWFKALLLLVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISAQTFADWQKKAADIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFSSEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMVNANGYTQRLPQLFQALLEGY
                     FSYTATEDQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPDEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(30616..33984)
                     /gene="recC"
                     /locus_tag="ACHKTW_000922"
     CDS             complement(30616..33984)
                     /gene="recC"
                     /locus_tag="ACHKTW_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HFO6461990.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LDREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHQLGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNPLLASWGKLGRDYIYLLSDLDSSQELDAFVDVMPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEDPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FRTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(33997..34320)
                     /gene="ppdC"
                     /locus_tag="ACHKTW_000923"
     CDS             complement(33997..34320)
                     /gene="ppdC"
                     /locus_tag="ACHKTW_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HFO6461991.1"
                     /translation="MSASLKNQQGFSLPEVMLAMVLMVMIVTALSGFQRTLMNSLASR
                     NQYQQLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGGREGEMTRLH
                     CPNRQ"
     gene            complement(34305..34712)
                     /gene="ygdB"
                     /locus_tag="ACHKTW_000924"
     CDS             complement(34305..34712)
                     /gene="ygdB"
                     /locus_tag="ACHKTW_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HFO6461992.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCSQYAGTDAQVCLRLLADNEALLIADYEGVSL
                     WRTGEVIDGNIVFSPRGWSDFCPLKERALCQLP"
     gene            complement(34709..35272)
                     /gene="ppdB"
                     /locus_tag="ACHKTW_000925"
     CDS             complement(34709..35272)
                     /gene="ppdB"
                     /locus_tag="ACHKTW_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HFO6461993.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVHQDVSGFSPVL
                     TVNMRAASKSEPQTVVNASYSVTGFNL"
     gene            complement(35263..35733)
                     /gene="ppdA"
                     /locus_tag="ACHKTW_000926"
     CDS             complement(35263..35733)
                     /gene="ppdA"
                     /locus_tag="ACHKTW_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HFO6461994.1"
                     /translation="MKTQRGYTLIETLVAMLILVMLSASGLYGWQYWQQSQRLWQTAS
                     QARDYLLYLREDANWHNRDHSISVIREGTLWCLVSSAAGANTCHGSSPLVFVPRWPEV
                     EMSDLTPSLAFFGLRNTAWAGHIRFKNSTGEWWLVVSPWGRLRLCQQGETEGCL"
     gene            complement(35917..36711)
                     /gene="thyA"
                     /locus_tag="ACHKTW_000927"
     CDS             complement(35917..36711)
                     /gene="thyA"
                     /locus_tag="ACHKTW_000927"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFO6461995.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGIKAPVAI"
     gene            complement(36718..37593)
                     /gene="lgt"
                     /locus_tag="ACHKTW_000928"
     CDS             complement(36718..37593)
                     /gene="lgt"
                     /locus_tag="ACHKTW_000928"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFO6461996.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(37744..39990)
                     /gene="ptsP"
                     /locus_tag="ACHKTW_000929"
     CDS             complement(37744..39990)
                     /gene="ptsP"
                     /locus_tag="ACHKTW_000929"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFO6461997.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDVTLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSRIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDYAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(40003..40533)
                     /gene="rppH"
                     /locus_tag="ACHKTW_000930"
     CDS             complement(40003..40533)
                     /gene="rppH"
                     /locus_tag="ACHKTW_000930"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HFO6461998.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNASAYRRKRG"
     gene            complement(40886..41032)
                     /gene="ygdT"
                     /locus_tag="ACHKTW_000931"
     CDS             complement(40886..41032)
                     /gene="ygdT"
                     /locus_tag="ACHKTW_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgdT"
                     /protein_id="HFO6461999.1"
                     /translation="MLSTESWDNCEKPPLLFPFTALTCDETPVFSGSVLNLVAHSVDK
                     YGIG"
     gene            41218..41907
                     /gene="mutH"
                     /locus_tag="ACHKTW_000932"
     CDS             41218..41907
                     /gene="mutH"
                     /locus_tag="ACHKTW_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HFO6462000.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            41976..42689
                     /gene="ygdQ"
                     /locus_tag="ACHKTW_000933"
     CDS             41976..42689
                     /gene="ygdQ"
                     /locus_tag="ACHKTW_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFO6462001.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASIAWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            42827..43045
                     /gene="ygdR"
                     /locus_tag="ACHKTW_000934"
     CDS             42827..43045
                     /gene="ygdR"
                     /locus_tag="ACHKTW_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HFO6462002.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            43153..44193
                     /gene="tas"
                     /locus_tag="ACHKTW_000935"
     CDS             43153..44193
                     /gene="tas"
                     /locus_tag="ACHKTW_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HFO6462003.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(44225..45418)
                     /gene="lplT"
                     /locus_tag="ACHKTW_000936"
     CDS             complement(44225..45418)
                     /gene="lplT"
                     /locus_tag="ACHKTW_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HFO6462004.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILFAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASTIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAAARPGQSWNLINMTRSFLNACTSLWRNGET
                     RFSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKL
                     VTLETVSRCMPAGILIGVVVLIFSLQHELLPAYALLMLIGVMGGFFVVPLNALLQERG
                     KKSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMIGIPVVPIGIGFGALFALAITALWI
                     WQRRH"
     gene            complement(45411..47570)
                     /gene="aas"
                     /locus_tag="ACHKTW_000937"
     CDS             complement(45411..47570)
                     /gene="aas"
                     /locus_tag="ACHKTW_000937"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008779 -
                     acyl-[acyl-carrier-protein]-phospholipid O-acyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008922 - long-chain fatty acid
                     [acyl-carrier-protein] ligase activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HFO6462005.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDPQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVAMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRMPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPEL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            48156..49187
                     /gene="galR"
                     /locus_tag="ACHKTW_000938"
     CDS             48156..49187
                     /gene="galR"
                     /locus_tag="ACHKTW_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HFO6462006.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLIVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIAANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(49194..50456)
                     /gene="lysA"
                     /locus_tag="ACHKTW_000939"
     CDS             complement(49194..50456)
                     /gene="lysA"
                     /locus_tag="ACHKTW_000939"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFO6462007.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFDVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDMLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSIPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDVGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            50578..51513
                     /gene="lysR"
                     /locus_tag="ACHKTW_000940"
     CDS             50578..51513
                     /gene="lysR"
                     /locus_tag="ACHKTW_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HFO6462008.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPAGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGVSVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSEHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(51500..52192)
                     /gene="ygeA"
                     /locus_tag="ACHKTW_000941"
     CDS             complement(51500..52192)
                     /gene="ygeA"
                     /locus_tag="ACHKTW_000941"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HFO6462009.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQVLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCTLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGQFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(52321..53739)
                     /gene="araE"
                     /locus_tag="ACHKTW_000942"
     CDS             complement(52321..53739)
                     /gene="araE"
                     /locus_tag="ACHKTW_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HFO6462010.1"
                     /translation="MVTINTESALTPRSLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGATFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
BASE COUNT        13141 a        14608 c        13367 g        12792 t
ORIGIN      
        1 tcactgatag atacataact tgtgtgtatc tttccgccct caaattatta cggcggtaaa
       61 tgattaagcc atcgccgata gacagatttc atttttacgg tcaggcacct tcccgggctg
      121 aactggctaa aagctgaatt atttgcattc ctccaggaga aatagatgga aacgactcaa
      181 accagcacga ttgcgtcgaa agactctcgt agtgcctggc gcaagacaga caccatgtgg
      241 atgctgggcc tttacggcac ggcaatcggc gcgggcgtgc tgttcctgcc aatcaacgcc
      301 ggtgttggcg gtatgatccc gctgatcatc atggctatcc ttgcgttccc gatgacgttt
      361 tttgctcacc gcggcctgac tcgcttcgta ctgtctggta aaaacccggg cgaagacatc
      421 accgaggttg tagaagaaca ctttggtatt ggcgcaggta aactgattac cctgctctac
      481 ttcttcgcta tctacccgat cctgctggtt tatagcgtgg caatcaccaa taccgttgaa
      541 agcttcatgt ctcaccagct gggtatgacg ccaccgccgc gtgcgattct gtcgctgatc
      601 ctgatcgtgg gtatgatgac catcgttcgc ttcggtgagc agatgatcgt taaagcgatg
      661 agtattctgg tattcccgtt tgttggcgta ctgatgctgc tggctctgta cctcatcccg
      721 cagtggaacg gcgcagcact ggaaacgctg tctctggaca ctgcatctgc aaccggaaac
      781 ggtctgtgga tgaccctgtg gctggcaatt ccggtaatgg tgttctcgtt caaccactct
      841 ccgatcatct cttctttcgc cgttgcgaag cgtgaagagt acggcgatat ggcagaacag
      901 aaatgctcga agatccttgc attcgcacac atcatgatgg tgctgaccgt aatgttcttc
      961 gtcttcagct gcgtactgag cctgactccg gcagacctgg ctgcggctaa agagcagaac
     1021 atctcgattc tgtcttacct ggctaaccac tttaacgcac cggttatcgc gtggatggct
     1081 ccgattatcg cgattatcgc tatcaccaaa tccttcctcg gccactacct gggcgcacgt
     1141 gaaggcttca acggtatggt gattaaatct ctgcgtggta aaggtaagtc tatcgaaatc
     1201 aacaagctga accgtatcac tgcgctgttc atgctggtaa cgacctggat tgttgccacc
     1261 ctgaacccga gcatcctggg tatgattgaa accctgggcg gcccaatcat cgcgatgatc
     1321 ctgttcctga tgccgatgta cgcaattcag aaagtaccgg caatgcgtaa gtacagcggt
     1381 cacatcagca acgtattcgt tgtcgtgatg ggtctgattg caatctccgc aatcttctac
     1441 tctctgttca gctaagtcct ttcgcgccgc tttcgggcgg cgcttcctcc gttttaacgc
     1501 gatgtatttc ctatgattag cgtattcgat attttcaaaa tcggcattgg cccttccagt
     1561 tctcataccg ttggaccaat gaaagcgggt aaacaattta ccgacgatct gattgcccgt
     1621 aatctgctta aagacgtgac ccgcgtggtg gttgacgtgt acggctcgct ctctctgacc
     1681 ggtaaaggcc accacactga tatcgccatt attatgggcc tggcgggtaa cctgccggat
     1741 accgtggata tcgattccat ccctggtttt attcaggatg tgaatactca tggtcgcctg
     1801 atgctggcaa acggtcagca tgaagtggag ttcccggttg atcagtgcat gaacttccat
     1861 gcagacaacc tttctctgca tgaaaacggt atgcgcatta ccgcgctggc gggcgataaa
     1921 gtcgtttaca gccagactta ctactctatt ggcggtggct ttatcgttga tgaagagcat
     1981 tttggccagc agaatagcgc accggttgaa gttccttatc cgtacagttc agcagccgat
     2041 ctgcaaaaac attgtcagga aaccgggctg tcactctctg gcctgatgat gaaaaatgag
     2101 ctggcgctgc acagcaaaga agagctggaa cagcacctgg cgaacgtatg ggaagtcatg
     2161 cgtggcggta ttgagcgcgg tatttccacc gaaggcgtgt tgcctggcaa actgcgcgtt
     2221 ccacgccgtg ctgcggcact acgccggatg ctggtcagcc aggataaaac caccactgac
     2281 ccgatggcgg ttgttgactg gatcaacatg tttgcattgg cagtgaacga agagaacgct
     2341 gctggcggac gcgtggtgac tgcgccgact aacggtgcgt gcgggattat cccggcagtg
     2401 ctggcgtact acgacaagtt tatccgcgaa gtgaacgcta actcactggc tcgttacctg
     2461 ctggtagcca gtgccattgg ttctctttat aagatgaacg cgtcaatttc tggtgctgaa
     2521 gtcggctgcc agggtgaagt tggtgtggcg tgctcaatgg cggcggctgg tctggcagaa
     2581 ctgttaggtg caagcccggc gcaggtgtgc atcgcggcgg aaatcgccat ggagcacaac
     2641 ctcggtctga cgtgtgaccc ggtcgccgga caggtccagg tgccatgcat cgagcgtaac
     2701 gccattgcgg cagtaaaagc ggtgaacgcc gcacgtatgg cgctacgccg taccagcgag
     2761 ccgcgcgtct gcctcgataa agttatcgaa accatgtacg aaacaggtaa agatatgaac
     2821 gccaagtacc gcgaaacctc tcgcggcggc ctggcaatga agatcgttgc ctgcgattaa
     2881 tcgctctcca gaggcctcgt tttgcgaggc ctcttcccga tttctcatcc agccgtagcc
     2941 tgttccggca tcgaatgtta cccttatcgc ctgatcttta agggggttat cgtggctgtt
     3001 catttgctta ttgtcgatgc actgaatctt attcgtcgca ttcatgccgt tcagggatcg
     3061 ccctgtgtcg aaacctgcca gcatgcgctc gatcagctca ttatgcacag ccagccaacc
     3121 cacgcggtcg ccgtttttga tgatgaaaac cgcagtagcg gctggcgtca tcagcgttta
     3181 ccagattaca aagcgggtcg accgccaatg ccggaagagt tgcacgacga gatgcctgca
     3241 ttacgcgccg cctttgagca acgcggcgtc ccgtgctggt caaccagcgg caacgaagcc
     3301 gatgacttag ccgccacgct ggcggtcaaa gtgacacagg ccgggcatca ggcaacgatt
     3361 gtttcgacag ataaaggcta ctgtcagtta ctttcaccga cattacgtat tcgtgattac
     3421 ttccagaaac gttggctgga tgcgccattt atcgataaag aatttggcgt tcaaccgcag
     3481 cagttgcccg attactgggg acttgcgggg atcagcagtt caaaggtacc gggtgttgcg
     3541 ggaatcggac caaaaagcgc cacgcagctg ctggtcgagt ttcagagtct ggaaggaata
     3601 tatgagaatc tggatgcggt tgccgaaaag tggcgcaaaa aattagaaac ccataaagag
     3661 atggcgtttc tgtgccgcga tattgcccgc ttacaaaccg atttgcatat cgacggcaat
     3721 ttacagcaat tgcggttggt acggtaacgg cgagccggat acgccgcaaa cgtcgtatcc
     3781 ggcattatca catcagcgca tttaccaggc ggtatggtaa agctctacaa tatcctcaag
     3841 cgttgcttca cgcgggttgc caccggtaca aacatcatcc agtgccgcct gcgccagtgc
     3901 cggaatgtct tccttgcgta caccaacatc acgcaaatgt ggcggaatac cgacatcacg
     3961 gttgagagca aacaccgctt caacagcggc attacgcgcc tcttccaggc tcataccttc
     4021 cactttcacg cccataacgc gcgcgatatc gcggtacttc tcaccggtaa agtcagcgtt
     4081 atagcgcatg acatgcggta acaggatggc gttcgcaaca ccgtgtggag tgttgtaaaa
     4141 cgcgcccagt ggatgcgcca taccatgcac caaccctaac ccaacattcg agaagcccat
     4201 acccgcaaca tactgcccga gcgccatttc ttctccggca tccttatcac cagcaaccga
     4261 tcctcgcagc gccccagcaa tgatttcaat cgctttaatg tgcagtgcat cggttagcgc
     4321 ccacgcgcca cgggtaatat acccctcaat agcatgagtg agcgcatcga cacccgtcgc
     4381 agctttcagc gctggaggca taccatccat catgtcagcg tcaataaacg ccacctgcgg
     4441 gatatcatgc ggatcaacgc aaacaaactt gcgccgtttc tcttcgtcag tgatcacgta
     4501 gttaatggtc acttctgccg cagtacctgc tgtggtagga attgccagaa tcggtacact
     4561 gggtttattg gtcggggaaa gcccttccag gctacgcaca tcggcaaact ccgggttgtt
     4621 gctgataatg ccaatcgctt tacaagtatc ctgtggagaa ccaccaccaa tagcgatcag
     4681 gtaatccgcg ccgctattct ggaatacacc gagcccttct ttgacgacag taattgttgg
     4741 gttgggcact acgccgtcgt aaatcgccca tgccagccct gcagcatcca tcttatcggt
     4801 cactttcgcc accacgccgc attgcaccag cgttttatcg gtgacgatca gcgccttctg
     4861 ataaccacgg cgtttcacct catcggttaa agccccaaca gcaccccgac caaaccatgc
     4921 cgtttcgttc agaatcattc tgttagccat catccttctc cttgttgctt tacgaaatta
     4981 ctcttcaatc cgtaacccat aggttttgaa tttctccagc actacggcaa tctcttcatc
     5041 gctcagcact ggcaccgggt ccgtaatcgc cagggtcgtc aggtaaagtt gcgccagcac
     5101 ttcaacttca tgcgccagcc ataacgcttt ttccagattc acctcacaag cgataagccc
     5161 atgatgttgt aacaaagttg ccttacgatt tttgagagcc tgcgcaacat gttcagaaag
     5221 ttcacgtgtt ccaaaggtcg cataaggcgc gcaaggaata gaattaccgc cagccgccgc
     5281 aatcatgtag tgaatagcgg ggatcgatcg gttaagaatg gaaactgccg tgcaatgaac
     5341 ggcatgattg tgaacaaccg cgttggcatc cggtctgctt tgataggccg ccatatggaa
     5401 acgccattcg cttgagggga gctttccttc ctcatgttta ccgttgccat caataaagac
     5461 aatatgcgac tccgtcagtt tttcatatgg aatgcctgta ggcgtaatca gcatcccatc
     5521 ctgataacgt acactgacgt tccccgctgt cccctggttc agtcccaggc gggtcatttc
     5581 caggcaagtg tcaataatct gacgagcaag tttatttcgt tccattagct acctctctct
     5641 gattcaaaac agggcaataa tgttgttcct ttcacactat tgaattagcc gtttaattac
     5701 ccaccatctt cttcctgatt aaccagaaag aaattcacaa gcttatattt tgtgacctgg
     5761 ttcaactaat cacagtaaat aactgcaagt tctcttttta taaccccatt aaaaatgacc
     5821 gctcttaaaa atatttatca aaacggtcat ttttctattc actcaagcca ggaatgcccg
     5881 ttttcggcat aaacaattaa tacggtcatc tggtttgtgt tttttatgat ttattttctg
     5941 aaacgggcat gaaatttcga ttattaaagt gatggtagtc acataaagtc accttccagc
     6001 taataagtgt gaccgccgtc atattacaga gcgtttttta tttgaaaatg aatccatgag
     6061 ttcatttcag acaggcaaat attcactgat atgaagcccg aactcgctgg ttttgcactt
     6121 ttgaaaacat aaccgattac gtgcttaagc ttctgaacct aagaggatgc tatgggaaac
     6181 acatcaatac aaacgcagag ttaccgtgcg gtagataaag atgcagggca aagcagaagt
     6241 tacattattc cattcgcgct gctgtgctca ctgttttttc tttgggcggt agccaataac
     6301 cttaacgaca ttttattacc tcaattccag caggctttta cgctgacaaa tttccaggct
     6361 ggcctgatcc aatcggcctt ttactttggt tatttcatta tcccaatccc tgctgggata
     6421 ttgatgaaaa aactcagtta taaagcaggg attattaccg gattattttt atatgccttg
     6481 ggcgctgcat tattctggcc cgctgcagaa ataatgaact acaccttatt tttagttggc
     6541 ctatttatta ttgcagccgg attaggttgt ctggaaactg ccgcaaaccc ttttgttacg
     6601 gtattagggc cggaaagcag tggtcacttc cgcttaaatc ttgcgcaaac atttaactcg
     6661 tttggcgcaa ttatcgcggt tgtctttggg caaagtctta ttttgtctaa cgtgccacat
     6721 caatcgcaag acgttctcga taaaatgtct ccagagcaat tgagtgcgta taaacacagc
     6781 ctggtattat cggtacagac accttatatg atcatcgtgg ctatcgtgtt actggtcgcc
     6841 ctgctgatca tgctgacgaa attcccggca ttgcagagtg ataatcacag tgacgccaaa
     6901 caaggatcgt tctccgcatc gctttctcgc ctggcgcgta ttcgccactg gcgctgggca
     6961 gtattagcgc aattctgcta cgttggcgca caaacggcct gctggagcta tttgattcgc
     7021 tacgctgtag aagaaattcc aggtatgact gcaggctttg ccgctaacta tttaaccgga
     7081 accatggtgt gcttctttat tggtcgtttc accggtacct ggctcatcag tcgcttcgca
     7141 ccacacaaag tcctggccgc ctacgcatta atcgctatgg cactgtgcct gatctcagcc
     7201 ttcgctggcg gtcatgtggg cttaatagcc ctgactttat gcagcgcctt tatgtcgatt
     7261 cagtacccaa caatcttctc gctgggcatt aagaatctcg gccaggacac taaatacggt
     7321 tcgtccttca tcgttatgac catcattggc ggcggtattg tcactccggt catgggtttt
     7381 gtcagtgacg cggcgggcaa catccccact gctgaactga tccccgcact ctgcttcgcg
     7441 gtcatcttta tctttgcccg tttccgctct caaacggcaa ctaactgaac atattttccg
     7501 aataaagtga ggaatctgta atgaaaaaaa tcagcttacc gaaaattggt atccgcccgg
     7561 ttattgacgg tcgtcgcatg ggtgttcgtg agtcgcttga agaacaaaca atgaatatgg
     7621 cgaaagctac ggccgcactg ctgaccgaga aactgcgcca tgcctgcgga gctgccgtcg
     7681 agtgtgtcat ttccgatacc tgtatcgcgg gtatggctga agccgctgct tgcgaagaaa
     7741 aattcagcag tcagaatgta ggcctcacca ttacggtaac gccttgctgg tgctatggca
     7801 gtgaaaccat cgacatggat ccaacccgcc cgaaagccat ttggggcttt aacggcactg
     7861 aacgtcccgg cgctgtttac ctggcagcgg ctctggcagc tcacagccag aaaggcatcc
     7921 cggcattctc catttacggt catgacgttc aggatgccga tgacacatcg attcctgccg
     7981 atgttgaaga aaaactgctg cgctttgccc gcgccggttt ggccgtcgcc agcatgaaag
     8041 gtaaaagcta tctgtcgctg ggcggcgttt cgatgggtat cgccggttcc attgttgatc
     8101 acaacttctt tgaatcctgg ctgggaatga aagttcaggc ggtggatatg accgaactgc
     8161 gtcgccgtat cgatcagaag atttacgacg aagccgaact ggaaatggcg ctggcctggg
     8221 ctgataaaaa cttccgctat ggcgaagatg aaaataacaa acagtatcaa cgcaatgccg
     8281 agcaaagccg cgcagttctg cgcgaaagtt tactgatggc gatgtgtatc cgcgacatga
     8341 tgcaaggcaa cagcaaactg gccgatattg gtcgcgtgga agaatcactt ggctacaacg
     8401 ccatcgctgc gggcttccag gggcaacgtc actggaccga tcaatatccc aatggtgaca
     8461 ccgccgaagc gatcctcaac agttcatttg actggaatgg cgtgcgcgaa ccctttgtcg
     8521 tggcgaccga aaacgacagt cttaacggcg tggcaatgct aatgggtcac cagctcaccg
     8581 gcaccgctca ggtatttgcc gatgtgcgta cctactggtc accagaagca attgagcgtg
     8641 taacggggca taaactcgat ggactggcag aacacggcat catccatttg atcaactcag
     8701 gttctgctgc gctggacggt tcctgtaaac aacgcgacag cgaaggtaac ccgacgatga
     8761 agccgcactg ggaaatctct cagcaagagg ctgacgcttg cctcgccgct accgaatggt
     8821 gcccggcgat ccacgaatac ttccgtggcg gcggttactc ttcccgcttc cttaccgaag
     8881 gcggcgtccc gttcaccatg actcgtgtca acatcatcaa aggcctggga ccggtactgc
     8941 aaatcgcgga aggctggagc gtggaattgc cgaaggatgt gcatgacatc ctcaacaaac
     9001 gcaccaactc aacctggcca accacctggt ttgcaccgcg cctcactggt aaagggccgt
     9061 ttacggatgt gtattcggta atggcgaact ggggtgccaa ccacggagtt ctgaccatcg
     9121 gccacgttgg cgcagacttt atcactcttg cctccatgct gcgtatcccg gtatgtatgc
     9181 acaacgttga agagaccaaa gtgtatcgtc cttctgcctg ggctgcgcac ggcatggata
     9241 ttgaaggcca ggattaccgc gcttgccaga actacggtcc gttgtacaag cgttaataca
     9301 ttactccctg atgtgatgcc cggtcgctcc ggctaccggg cctgaacaag caagagtggt
     9361 tagccggata aacaatgtta tccggctata ttgcaggagc gattatgaaa caagaagtta
     9421 tcctggtact cgactgtggc gcgaccaatg tcagggccat cgcggttaat cggcagggaa
     9481 aaattgttgc ccgcgcctca acgcctaatg ccagcgatat cgcgatggaa aacaacacct
     9541 ggcaccagtg gtctttagac gccattttgc aacgctttgc tgattgctgt cggcaaatca
     9601 atagtgaact gactgaatgc cacatccgcg gtatcgccgt caccaccttt ggtgtggatg
     9661 gcgctctggt agataagcaa ggcaatctgc tctatccgat cattagctgg aaatgtccgc
     9721 gaacagcagc ggttatggac aatattgaac ggttaatctc cgcacagcgg ttgcaggcta
     9781 tttctggcgt cggagccttt agtttcaata cgttatataa gttggtgtgg ttgaaagaaa
     9841 atcatccaca actgctggaa cgcgcgcacg cctggctctt tatttcgtcg ctgattaacc
     9901 accgtttaac cggcgaattc actactgata tcacgatggc cggaaccagc cagatgctgg
     9961 atatccagca acgcgatttc agtccgcaaa ttttacaagc caccggtatt ccacgccgac
    10021 tcttccctcg tctggtggaa gcgggtgaac agattggtac gctacagaac agcgccgcag
    10081 caatgctcgg cttacccgtt ggcataccgg tgatttccgc aggtcacgat acccagttcg
    10141 ccctttttgg cgctggtgcc gaccaaaatg aacccgtgct ctcttccggt acatgggaaa
    10201 ttttaatggt ccgtagcgcc caggttgata cttcgctgtt aagtcagtac gccggttcca
    10261 cctgcgaact ggatagccag gcagggttgt ataacccagg tatgcaatgg ctggcatccg
    10321 gcgtgctgga atgggtgaga aaactgttct ggacggctga aacaccctgg caaatgttga
    10381 ttgaagaagc tcgtctgatc gcgcctggcg cggatggcgt aaaaatgcag tgtgatttat
    10441 tgtcgtgtca gaacgctggc tggcaaggag tgacgcttaa taccacgcgg gggcatttct
    10501 atcgcgcggc gctggaaggg ttaactgcgc aattacagcg caatctacag atgctggaaa
    10561 aaatcgggca ctttaaggcc tctgaattat tgttagtcgg tggaggaagt cgcaacacat
    10621 tgtggaatca gattaaagcc aatatgcttg atattccggt aaaagttctc gacgacgccg
    10681 aaacgaccgt cgcaggagct gcgctgttcg gttggtatgg cgtaggggaa tttaacagcc
    10741 cggaagaagc ccgcgcacag attcattatc agtaccgtta tttctacccg caaactgaac
    10801 ctgaatttat agaggaagtg tgaaatgctg aaaacaattt cgccgttaat ttctcccgaa
    10861 ctattgaaag tgctggcaga gatgggacat ggagatgaaa ttattttttc cgatgctcac
    10921 tttcccgccc attcgatggg accgcaggtg atccgcgctg atggcctgtt ggtgagcgac
    10981 ttgctccagg cgattatccc gttatttgaa ctggacagtt atgcaccgcc gctggtgatg
    11041 atggcggcgg tagaaggtga cactctcgat cctgaagtag aacgacgtta ccgtaatgcg
    11101 ctttcactac aagccccgtg tcctgacatc atccgcatca atcgttttgc gttttatgaa
    11161 cgggcgcaaa aagcctttgc gatcgttatc acaggcgaac gagcgaagta cgggaatatt
    11221 cttttaaaaa aaggggtaac accgtaatct cataccggta cgcccgcatg acgcgggcgg
    11281 ttatcgaatg atggggtgaa aaatatgaaa gcggcacgcc agcaagcgat agtcgacctg
    11341 ctgctgaacc ataccagcct gaccacggaa gctctctctg aacagctaaa ggtcagtaaa
    11401 gaaaccattc gtcgcgatct caatgaatta cagacgcagg gtaaaattct gcgcaatcat
    11461 ggacgcgcta aatatatcca ccgtcaaaat caagacagtg gcgatccctt tcacatcagg
    11521 ctgaaaagcc attatgcgca taaagcagat atcgcgcgcg aggcgctcgc gtggattgaa
    11581 gaagggatgg tgatagcctt agacgccagt tcaacttgct ggtatctggc acgccagttg
    11641 cctgacatca acattcaggt cttcaccaat agccatccga tttgccatga actcggtaaa
    11701 cgcgaacgca ttcaactgat cagttccggc ggcacacttg agcgcaaata tggctgttac
    11761 gtcaatccct cgctgatttc ccaacttaaa tcgctggaaa tcgatctgtt tattttttct
    11821 tgtgaaggga tcgatagcag cggcgcactg tgggactcca atgcgatcaa cgctgattac
    11881 aaatcgatgc tattaaaacg tgccgcgcaa tcgttgttat tgattgataa aagtaaattt
    11941 aatcgttcag gggaagcccg catcgggcat ctggatgagg taacgcacat tatttctgat
    12001 gagcgccagg ttgcaacttc tttggtaaca gcctgacgga aagggtagca ggccggagac
    12061 gacgccccgg ccttgcctgt tatcgctcgt cgcgacgacc acccaccgca gcccagatgc
    12121 ggcggacgtg caccgtcact tcttcgcgat cgtgatacaa ctgccgcgcc tgaatctgag
    12181 catttatgcc atgttcatca agctgtgcct gaatatacgc cagattgtgt gacacttctt
    12241 cgtagcgttt tttcatcggc agtttgaggt tgaaaatggt ttcacggcac cagccattaa
    12301 ccagccactg cgccatcaat gccgcaactt tcgccggttt ttcaaccata tcgcatacca
    12361 tccaggagat attgctgcgc gtcggacgga atttgaaacc gtcttcccgc agccacgtca
    12421 cctgtccggt atccatcaga ctttgcgcca tcgggccgtt gtcgacggaa taaacccaca
    12481 tgttgcgctt caccagttgg taggtccagc cgccagggca agcgcctaaa tccaccgccc
    12541 acatcccgtt cgccaggcgt tcatcccact catccgcagg aataaacaca tgaaatgcct
    12601 cttccagttt gagcgtggaa cgactcggcg catctgccgg aaatttcagg cgcggaatgc
    12661 ccatatagaa cggcgaatta ttgttgctgt atgagtaacc ggtatagcag cagcctggtg
    12721 caataaagaa tacatgcaca accggacgct tcggcgtttc atagttcgcc agcaccccgg
    12781 catcgcgcaa ggcagcgcgt agcggaacag taaatttacg gcagaatttc agtaactctt
    12841 tgctttcgtt ggtatcggca acttcaacac gcagttcacc gcccttctct actacgccct
    12901 gtaacatgcc gacaatgggg gtaatacgat cttctggcgg caaatgctgc aggagttccc
    12961 ccaccacaaa ccactggcgg gcaaaaatta atgaactgaa cggcagctca cggattaact
    13021 tatcgccatc atcaggttga taacattcat aaatgacata acccgcattc tctttcacgc
    13081 gggcaaaacc gaaaatttcc cgctggccgg ctttatcggt aatttctgcg gcgcactctt
    13141 tttcaaagcc cggacggcac agcaatacaa ccttattcat gacttacgcc cttgcgcttt
    13201 aaacggatag caccaactaa cattaacgcc cagcccgcga ggaagctcac gccgccaacc
    13261 ggagtgacaa acgcccacaa acgcagatgg gacagcgcca ggcaataaag gctgccgctg
    13321 aacaacaccg tgcctaacgc gaggaaaacg ctactccagt aaaaccagat actgatgcga
    13381 cgctgcattg ccaccgccag acctaagatc gccagcgtat gaaacgcctg gtattcgagg
    13441 ccggtctgga tccagcccat ctcaacggcc cccatggttt tacttaacac atgcgcgcca
    13501 aaagcgccca gagccacaaa aatgaagccg ctaatggcgg cgaaaatcag cataaaacgg
    13561 ctggtcatgg tcgtacccta cgtaaattat tgttcataac gaaagcggaa tttttcttgt
    13621 tcagcagcgg ctttcgccag gatccattgg cgaaaggcgg ctattttacc cagttctgcc
    13681 tggctgtcat gacaaaccag ataaaaagcg tttttactga ccagaacatc attaaacggg
    13741 caaacaagac gtccggcctc gatttcagat tgcgccatca cgttatttgc cagcgccact
    13801 ccctgcccgt gaatagccgc ttgcagcacc atggcgctat gactaaaaat tggcccttgc
    13861 tgaacgttga tatgatttaa ccccaactgt cgggtatatg tctgccagtc acggcgtgac
    13921 gcatcatgta ataacgtatg tttagccaga tcttccgggg tcttcaaggg tttttcgcct
    13981 gtcagcagta gcggcgaaca caccggcaat aaatattcgg cgtacagttt ttccacccgt
    14041 agccccggcc agttgccccg accataaaat atcgccacat caacatcatc cgccagctta
    14101 tcttcctgac gatcaaccgc ctggattcga acgtcaattc ccggataagc tgaattaaag
    14161 ctggaaagtc gcggaaccaa ccaatgaatg gcgaaactgg ggagtaaact gaccgtcaac
    14221 gcccccttgg cgctacgggc ctggagttta cgcgtcgctt cggttaattg cgaaaatatc
    14281 tctttgatat cgaggaaata gctttgccct tcctcggtca gcaggagtga acgattacgg
    14341 cggcggaaca gttttagccc caaaaaatcc tcaagagact tgatttgatg acttactgcg
    14401 gcctgggtca caaaaagctc ttctgctgcg cgagtgaaac ttaaatggcg tgctgcggca
    14461 tcaaaaactc gtaaggcatt tagcggtggt aatcgtttag acatggcttt taaactttga
    14521 tgttaaatga atttaacaat tagatcacac tgtgtaacct attagttttt ttaatctgag
    14581 ccattataaa ttgtccgttg agcttctacc agcaaatacc tatagtggcg gcacttcctg
    14641 agccggaacg aaaagtttta tcggaatgcg tgttctggtg aacttttggc ttacggttgt
    14701 gatgttgtgt tgttgtgttt gcaattggtc tgcgattcag accatggtag caaagctacc
    14761 ttttttcact tcctgtacat ttaccctgtc tgtccatagt gattaatgta gcaccgccta
    14821 attgcggtgc ttttttttac cttgcgatcg cgaattactg atccagttcg accatctctt
    14881 tcacgtcagt acggttgatc tgctgtttgt tgccattagc gtctttatac gaaatcatac
    14941 cggtatcgtt atcagtctgt ggtttgccgt cagagacgat ggtacgtccg tcattggtgt
    15001 gcatcacata gttcgaaccg gaacaggcgc tcagggcaaa agtcagcata caggcagaaa
    15061 taattgcggc agtctttttc atcatcttct ccttaaagcg attaataaat cttaatagta
    15121 ttgattaaca ggctaaaatt aacgcctaac actattcagc atatgttact tacgcggctt
    15181 cgccaggata tccagataat tctgatggtt agcactctcc ttgtatcaaa gtgaattttg
    15241 cgtcacgatc ggtgcatcaa gccgaggagt accatgaacg tttttaatcc cgcgcagttt
    15301 cgcgcccagt ttcccgcact acaggatgcg ggcgtctatc tcgatagcgc cgcgaccgcg
    15361 cttaaacctg aagccgtggt tgaagccact cgacagtttt acagcctgag cgccggaaac
    15421 gtccatcgca gccagtttgc cgaagcccaa cgcctgaccg cgcgttatga agctgcacga
    15481 gagaaagtgg cgcaattact gaatgcaccg gatgataaaa ctatcgtctg gacgcgcggc
    15541 accactgaat ccatcaacat ggtggcacaa tgctatgcgc gtccgcgtct gcaaccgggc
    15601 gatgagatta ttgtcagcgt ggcagaacac cacgctaacc tcgtcccctg gctgatggtc
    15661 gcccaacaaa ctggagccaa agtggtgaaa ttgccgctta atgcgcagcg actgccggat
    15721 gtcgatttgt tgccagaact gattactccc cgtagtcgga ttctggcgtt gggtcagatg
    15781 tcgaacgtta ctggcggttg cccggatctg gcgcgagcga ttacctttgc tcattcagcc
    15841 gggatggtgg tgatggttga tggtgctcag ggggcagtgc atttccccgc ggatgttcag
    15901 caactggata ttgatttcta tgctttttca ggtcacaaac tgtatggccc gacaggtatc
    15961 ggcgtgctgt atggtaaatc agaactgctg gaggcgatgt cgccctggct gggcggcggc
    16021 aaaatggttc acgaagtgag ttttgacggc ttcacgactc aatctgcgcc gtggaaactg
    16081 gaagctggaa cgccaaatgt cgctggtgtc ataggattaa gcgcggcgct ggaatggctg
    16141 gcagattacg atatcaacca ggccgaaagc tggagccgta gcttagcaac gctggcggaa
    16201 gatgcgctgg cgaaacgtcc cggctttcgt tcattccgct gccaggattc cagcctgctg
    16261 gcctttgatt ttgctggcgt tcatcatagc gatatggtga cgctgctggc ggagtacggt
    16321 attgccctgc gggccgggca gcattgcgct cagccgctac tggcagaatt aggcgtaacc
    16381 ggcacactgc gcgcctcttt tgcgccatat aatacaaaga gtgatgtgga tgcgctggtg
    16441 aatgccgttg accgcgcgct ggaattattg gtggattaat gacaaacccg caattcgccg
    16501 gacatccgtt cggcacaacc gtaaccgcag aaacgttacg caataccttc gcaccgttgt
    16561 cgcaatggga agataaatat cgccagttga tcatgctggg gaaacagctt ccggctttgc
    16621 cagacgagtt aaaagcgcag gctaaagaga ttgccggatg cgaaaaccgc gtctggctgg
    16681 gatataccgt gactgaaaac ggtaaaatgc acttctttgg cgacagcgaa gggcgcattg
    16741 tgcgcggcct gttggcggtg ttgttgactg ccgttgaggg gaaaaccgtc gccgaattgc
    16801 aggcacagtc gccgctggca ttgtttgatg agctgggatt gcgtgcacag cttagcgcct
    16861 cacgcagcca ggggttgaat gcgttaagcg aggcgattat cgctgcggcg aagcaggttt
    16921 aagcacagaa ccaggtcgga taagacgttc gcgtcgcatc cgacctgatt gtttaaccct
    16981 gacgcgccgc tttcgccatc atcttcttca gcgcatgaga aaccgcaaca aacccaaagg
    17041 tggcggtcac catcgttgcc gcgccaaatc cagatgcaca atccatccgc ttcggccctt
    17101 ctgccgtggc tttcatcgca cacacagtac cgtctgactg cgggtacacc agcgcttcag
    17161 tagaaaacac gcaatcgacg ccgagcttac ctttactgtt tttcactacg ccaaaatcgc
    17221 ttttcaggcg ctcgcgcaac ttcgccgcca gcggatcctg aatcgttttc gccagatcgg
    17281 taacctgaat ctgcgacggg tcaatctgcc cacccgcgcc accggtcgtg accagcggga
    17341 ttttattgcg ccgacaataa gcaatcagcg ccgctttggg ccgtacacta tcaatggcat
    17401 caatcacgta cgaataaccc acgctcatat actgcgctac gttatccggc gtcacgaaat
    17461 catccaccac cgttacacgg cactccgggt taatctggcg aatacgctcc gccatgactt
    17521 ccgcttttgc cagcccgacg ttatcgcgca gagcgtgaat ttgccgattg gtattggtga
    17581 cgcacacatc atccatatcg ataagcgtga ttgcgccaat ccccgtgcgc gccagcgctt
    17641 ccgccgccca ggaaccgaca ccaccgatac caaccacaca aatatgcgcg tcagcaaaca
    17701 gttgcaacgc tttttcacca tacagacgcg ctgtgccacc aaaacgctga cgccatgcat
    17761 cactaattac cacagacata gcacctcaga attaaaaagg gtgaggttat ccctcaccct
    17821 ggcgtatccg taaaaacggt atacagaata acacatcagc cgctaaagac gttacctgcg
    17881 cccggggcgg ttttcagcac ccagacacgt ccatagtggt tgtaccaacc tgcgcgatgt
    17941 ccggcttccg gcccgatccc ttgatagata tcgaagtgtt ggcctttgat tgcgccaccg
    18001 acatccagcg ccaccatcag acgcaattcg tactgaccgt taaatttgcc gttattatcc
    18061 agcagcggca cttctgccag caaggtagta cctggcggaa taattgaacg atcggaggca
    18121 acagaagcac ggccaaccag cggcaccgca cttgcccctt tcaccggtgc aaaagattgc
    18181 ggtttaaaga aaacgaaaga cgggttctgt tccagcagct cgcgaacctc ggcttcactg
    18241 tgtgtttcgc cccaatgacg aatcgcctgc atcgacatat cttctttttt cacttcgcca
    18301 cggtcgatca gcaccttacc aatgctgcga taggcatgac cgtttttccc tgcatagctg
    18361 aaaaagttaa gcggactgcc atcaccaaag tcgatatacc cgctaccctg cacatccata
    18421 atgaagttat ccatcaggga gttgctgtaa gcgagaatat atttatcact cagcgcccct
    18481 gcgtagacct ccgcacgaga cggcagacga ccacgttttg gcggcatacg gtaaatagga
    18541 tactggaact cgccctggcg ggtatggcgc gcctgaatta ccggcgtgta atagcctgta
    18601 aactgcacgt taccatagtt gtcggcacct tccatctgcc aggcatcaat gccgaactgg
    18661 cgcatattgc gggtatcacc gcctgcgcgc agccactctt gcaccgcgtt ataaacatta
    18721 ctctggttgc catacagacg cggtgacgaa ttacggatat ggttaatttg ctcggcaaaa
    18781 tcaccggcgt taatcggcgc gccaacggca tctggctggt tcaccagaga gaaaggctgg
    18841 gtaaatttcc cgtctttata ttgctgtccg cgatcggttg gtttggaaga gcaggcggca
    18901 agcattgcca caaccgtgcc cataagaagg tactttaccc aacgtccttt cattgttctc
    18961 ttcttcaggt taaaaaataa ggcgcaacga agataacaaa ccgccgcgtt taatgaaatg
    19021 agcagcctct ccctgacgca aattttgcac aaaaaatagg ctttagtggt ttgtttttgt
    19081 tcaaaatcat gctaaatccg tgatcggggt aaaaaaaagg ttgcatgaaa acgcgagcgg
    19141 agtatagtgc gcatccacgg acgcggggtg gagcagcctg gtagctcgtc gggctcataa
    19201 cccgaaggtc gtcggttcaa atccggcccc cgcaaccaat tattgaacac cctaacgggt
    19261 gtttttttgt ttctgctctc ccataaaaaa agcgccattc agcgcctttt tatcatcccc
    19321 ttctcgccag cgtcgcccca tcggcaaaat acgctttaat tcccgcaaga atagactccg
    19381 caacttcctg ctggaaagtc gccgttttca gtttacgctc ttcctcaacg ttactgataa
    19441 acgccgtttc gaccagaatg gagggaatat caggcgcttt cagcacggca aacccggcct
    19501 gttcaacctg atttttatgc agcttgttga ttttaccgag cttattcagc accgctttac
    19561 caaacttaag gctgtcggca atggtcagcg actgcaccat atcgaacatg gtgtggtcga
    19621 catagcggtc accgcttttg ctcacgccac caatcaagtc cgaggcgttc tgggtctgtg
    19681 ccagatattt tgccgcagta ctggttgcgc ctttggttga gagcgcaaac actgaggaac
    19741 cgctcggctg gcgactggta aaggcgtcgg catggataga gacaaacagg tcggcacgct
    19801 gtttctgggc ttttgctacg cgcacttgca acggaatgaa gatgtcttca ttgcgcgtca
    19861 tgtacacctt catattgccc tctttctcga tcagagagcg cagacggcga gctatttgca
    19921 ataccacgtc tttttcgcgc gttttgtatt tccccaccgc accggagtct tcgccgccgt
    19981 ggccagggtc aagcataata acaataggac gatcgcgccc tgctttaccc ggttgtggac
    20041 cactttgtgc tggcggcacc tgcttttcga ggtcgccttt gttgtaatcc tccagcagcg
    20101 ccagcagcgg gtcctgcata tcctgtgcat tggccggata gaggtccatc accagacgct
    20161 ctttaaaccc ggcgaccggc gcaagggcaa acagctgcgg ttttacgttt tgctttaatt
    20221 caaaaaccat ccgtacggtc tgcgggtcaa attgcccgac gcgcgccgac ttgatgaacg
    20281 ggtcgtctgc acgaatttgt gccgccatcc ccttgagtac cgagttcagg tttacatctt
    20341 cgatatccac caccacgcgt tccgggttac tcaacgcgaa ctgcttatat ttcagctgac
    20401 gatttgattc taccgtcacg cgggtgtagc tggacgcagg ccagacgcgc accgccacga
    20461 cctggctaac cgcagccagg ctgacctgac ttacgctcaa tagccacata gcgcccgcgc
    20521 cttgcagtaa acgacgacgg ctgattgcag tgttggatcc tgacatgcct ctcccgagca
    20581 aaagaaatct aagctgtgta acaagtaaac gactaatttg accggaaact ttagcgaaag
    20641 acgcataatc tgtcatctaa taaacggtaa acattctttt tatattcacg gcattactga
    20701 taaaaaagtc gctctcgcat aaaatttaca cttgcaccct gcgaaaaaac agaataaaaa
    20761 tacaataatt tcgaataata atgcaaaggg gtgtgccgtg gtaaaggaac gtaaaaccga
    20821 gttggtcgag ggattccgcc attcggttcc ctatatcaat acccaccggg gaaaaacgtt
    20881 tgtcatcatg ctcggcggtg aagccattga gcatgagaat ttctccagta tcgttaatga
    20941 tatcgggttg ttgcacagcc tcggcatccg tctggtggtg gtctatggcg cacgtccgca
    21001 gatcgacgca aatctggctg cgcatcacca cgaaccgctg tatcacaaga atatacgtgt
    21061 gaccgacgcc aaaacactgg aactggtgaa gcaggctgcg ggaacattgc aactggatat
    21121 tactgctcgc ctgtcgatga gtctcaataa cacgccgcta cagggcgcgc atatcaacgt
    21181 cgttagtggc aattttatta ttgcccagcc gctgggcgtc gatgacggcg tggattactg
    21241 ccatagcggg cgtatccggc ggattgatga agacgcgatc catcgtcaac tggacagcgg
    21301 tgcaatagtg ctaatggggc cggtcgctgt ttcagtcact ggcgagagct ttaatctgac
    21361 ctcggaagag attgccactc aactagccat caaactgaaa gctgaaaaga tgattggttt
    21421 ttgctcttcc cagggcgtta ctaatgacga cggtgatatt gtctcagaac ttttccctaa
    21481 cgaagcgcaa gcgcgggtag aagcccagga agagaaaggc gattacaact ccggtacggt
    21541 gcgctttttg cgtggcgcag tgaaagcctg ccgcagcggc gtgcgtcgct gtcatttaat
    21601 cagttatcag gaagatggcg cgctgttgca agagttgttc tcacgcgacg gtatcggtac
    21661 gcagattgtg atggaaagcg ccgagcagat tcgtcgcgca acaatcaacg atattggcgg
    21721 tattctggag ttgattcgcc cgctggagca gcaaggtatt ctggtgcgcc gttctcgcga
    21781 gcagctggag atggaaatcg acaaattcac cattattcag cgcgataaca cgactattgc
    21841 ctgcgccgcg ctctatccgt tcccggaaga gaagattggg gaaatggcct gtgtggcagt
    21901 tcacccggat taccgcagtt catcacgggg cgaggttctg ctggaacgca ttaccgctca
    21961 ggcgaagcag agcggcttaa gcaaattgtt tgtgctgacc acgcgcagta ttcactggtt
    22021 ccaggaacgt ggatttaccc cggtggatat tgatttactg cccgagagca aaaagcagtt
    22081 gtacaactac cagcgtaaat ccaaagtgtt gatggcggat ttagggtaag cgatgaaaat
    22141 tgtcggatgc gacatgcgta acactcgtgc gtcgcatccg gcaattacgt ttattcccgc
    22201 gaactaaaca acgccgccag accactgcgc cgctcagtac gagtggcgat tgccgcactt
    22261 aatatacgct catcggcata cagcgacaga cggcgacgcg cgcgggtcac cgcggtgtat
    22321 accagctctc gcgttactac cggcgtgcgt tggctcggta aaatcaacgc cgcatggtcg
    22381 aactccgatc cctgcgattt atgtaccgtc atcgcccacg tagtttcgtg ctctggcaag
    22441 cgactcggtt gcacagactt aatattgccg tccggcatcg caaaccagac gcgcgtcccc
    22501 tgcccgcgat ccagcgcaat accgatatcg ccattaaaca acccaagcgc gctgtcatta
    22561 cgggcaatca ttaccggtcg accttcgtac caacgagagt gcggatgacg atgaattttg
    22621 cgcttctgtt gcataaactg ctcaattcgc tcattcagtc cagccacgcc aaacggccct
    22681 tcccgcaggg cgcacaaaag ttggtactca ttgaacgcct gaatgattaa atccggctcg
    22741 gcacgcgctt gcagcagatc cagataacgt ccataacccg caagagcttc ctcaagcatc
    22801 gcaatataat cttctccgct ctgtaaaagc cgtttttcga tatcagtaaa atcctgctga
    22861 aaaacggttt tcactgccgt tttatcacca cggttgatcg ccgcagctaa ctgaccaatg
    22921 ccagaatcac tgccgaaacg atagcttttt tgcagcaggc agagactgtc gcgcaaagat
    22981 gccgcttctg tgccagttcc tgccggaacg tgagttcccg tcaggcggct tagctgcccg
    23041 gcacgctcgg cggtaaagcc ggcattggca taagcgcaga tatcgcccag cacagcccca
    23101 gcctcaaccg aggccagttg atcacgatcg ccgagaaaga tcactcgcgc atgatcgggc
    23161 aaggcgtcga tcagtctcga catcataggc agatcgatca ttgacgcttc atctaccacc
    23221 agcacatcaa gatgcagcgg gttaccggca tgatgacgta aacgctggct acccggctgc
    23281 gcacccagca atcggtgcaa agtactggca tcttccggaa tgcgtttctt ttgttcatcg
    23341 gtcagcggta actgtcgcaa agccttgccg agagattcgg ttaagcgcgc ggcagcttta
    23401 cccgttggtg cagccagacg gatacggcag cgttcgccgt cggccatttg aattaacgct
    23461 gccagtaact tcgccacggt ggtcgtttta ccggtgcctg ggccgccgga aatcaccgag
    23521 atccgccgcg tcagcgccac tgccgctgca actttttgcc agttaatttc atcgcttact
    23581 ggaaaaagtt tgtccagggt ttgcgccagt agagcttcat caacctcaat ggcatgattc
    23641 acctcattga agaagcgcgc taccgtccgc tcgttacacc acatgcgatt caaataaaga
    23701 cgatcgccac agaggatcat cggcgtgggt tcatctcccc tgctgaccgc ttgagaagcc
    23761 agcaagcatt cttcccaatt ttgtagctca ccgatttcac tgacacaggt cgccaacagc
    23821 ggatgcgacg cctcgttatt ttccagtcgt gaaagcggca aacagacgtg tccctctccg
    23881 gcatcatgac ttaacagtgc cgccgcgagg gtgacggcag gatgttcatc tcccgccacg
    23941 gtcagggcaa attgcacatc cagcgggcgt agctgtttgt gctccacagc ttccagtaat
    24001 tgcttttgca atttcattac gcctcctcca gggtcatacc ggcaaacatc tcatccatca
    24061 gggcaatcaa cccggcgttg ggtcgggttg tgtaaatccc ttgttgcgga tgttctttat
    24121 caacgccacg caggaacaga tagataacgc cgccaaagtg acgatcatag tcgtaatcag
    24181 caatgcgatg gcgcagataa cgatgcagcg ccagggtata aagctgatat tgcagatcat
    24241 agcggtgtgc ctgcatcgct gctgccatag cctgttgggt gtaagccgaa ctgtcttcac
    24301 ccagccagtt ggatttatag tcgagcaggt aataacgccc ttcgtggcgg aataccaggt
    24361 cgataaagcc ttttaacatg ccgcgtacct gcatgaactc cagcggcggg cagccagcgg
    24421 atagcgggtc aaactggcga attagtgcat caagctggct ggcgatgagc ggttcgctaa
    24481 tcggcaggta aaactccatc tccacctgtt tatcgcggtc ggaaagctga ctcaggctaa
    24541 cacccgtttc attgagaggt gcctggagga cagccgtgat ccactcggtc aacaccggtt
    24601 cccactgcga ttcaaagccg ccgagttcca gtttttcctg tacccagttc gggtcaaccg
    24661 gctgggtaaa atcgaggtct tcaaacaaac tgtgcaagaa tgtccccggt gacgcaccgc
    24721 gcgggaactg atgcggtgtt aacgtcggtt cttcaacgac gctgaccacg cctgcggcat
    24781 caacatccag ccgtggcatc agatcctgag cgataccgtg accacgctgc tgcaagccgg
    24841 agtagctggt gacacgccag ttatcgccgg gcaatcgttg tagtgtcctc gcattcagtt
    24901 ctgcagttaa agcatcatta acctgccagg gctggttatc accagtttgt gccgtttgcc
    24961 aggcaatatc atcatcgcat aacgcttcaa tacaggtgcg aagccctgcc gcatcttgcg
    25021 gttccccttt ttgcagcaaa cgcccgagcg cactttggtg gacgtcggtg tcaccttttt
    25081 tatcgccacg acggcgcacc agcggtgcaa cgccgagact gcaatgccaa accgaacgcg
    25141 tcagcgccac gtaaagcaaa cgcagatctt ccgccagacg ttcgacctcc gcgaggtcga
    25201 cgctttctgg cgcagcgtta agatccaaaa ctgcctcaaa cgagtggcga tcgtgataaa
    25261 acgcctgatc ctggacgcgg aaattggtga taaacggcag ccagaccaat ggatattcca
    25321 gccctttcga tttgtggatc gtgacaatct gcaccagatg tttatcactt tcgagacgca
    25381 tttgttggct ggaggcatta ctgtctggct cgaggatatg ttgcgataac cagcgtacca
    25441 gcgcatgttc actttccagc tgcgttccgg cttcttgtag cagttcgctg atatgcaaga
    25501 tatcggtaag acgccgctca ccgcctgccg ttgccagcaa gttttcagca atgttacgcg
    25561 ccgacatcag cgcccgcagc atcggcataa cgccacgttt gcgccagatt tgccgataac
    25621 catcgaactc ttcgactacc acatcccacg catgttcgtc attgttcagc gtttcgatat
    25681 ccagcgcgtt cagccccatc attgacgttg ccagcgcact acgcagggtg ttctcacgtt
    25741 cgggcgtcat caccgcctgc aacaaccaaa gcatttcctg cgcttccaga gtttcaaaaa
    25801 cactgtcgcg gttcgaaagg taaacggaag ggatttccag caacgttaag gcgtcgcgca
    25861 cctgggcggc ctcctggcgg ctgcgcacca gcacactgat gtccgaagca cgcaccggac
    25921 gcgcgtcgtc gccgttcatc agcaacgctt cgccccgctg tccggcttgt agccagtcgc
    25981 ggatttgcgc agcacatacc tgcgccatgg tactttgata gtcgccaacg ccgcagcttt
    26041 cgccttccat cagccacatt ttcatcgcag gctgcgtttc acctttaaac acaaaacgta
    26101 acgcctgatt tttcccggct gatttcactg gaataaacgg tatttcgcga aacatgaacg
    26161 cgtcatcagt ctggctgaaa agcttgttca cactgttcac cattcctggt gcggaacgcc
    26221 agttggtgtc taaagtgtag tgggcgtgaa cttcgctacg cgccttcata taagtgaaga
    26281 tatccgcacc ccggaatgca tatatggcct gtttcgggtc gccaatcagc aacaatgcgg
    26341 tttccggctg atggtgccag atacggcgaa aaattcggta ctgctggggg tcggtatcct
    26401 gaaattcatc aatcattgcc accgggaatc gcgtacggat cgccgctgcc aacacctcgc
    26461 cgctttcgct acgcagcgcg gaatcgagcc gacttaacat gtcatcaaaa cccaattcgc
    26521 cacggcggcg tttttcacgc gctactgttt cgcggatctc agccaatgcg cgggttatca
    26581 ccagatcacg gatcgacaat ggttctgcaa gcagttgttc gatcgcctca aacagtggat
    26641 gtcgcggggt ttcccccccg gctttcgtgc gatcttctaa gaaacgctga gagaattttt
    26701 ccagcgactc cggtaactga taactgttgg tctcttcttc tgcccaggcg ctgatcttct
    26761 cgatccattt agcctgattg ctacggttaa acttgcgtcg atcaatacca gaagattcga
    26821 tcagcgcatc cagttcaccc actgcgtcgc gccactgctg ttttaccgta tcaatacgcg
    26881 ccacaatttg cgcgtggcgg gaagccagcg tttcatcatc gggcggcggt gctttgataa
    26941 ccggtgcttc gccttgcaga taacgattaa tatcgcgcag caacgcctgc ggccctttcc
    27001 aggtttcaaa gaccaccagg gcaatttcac gcggcagcgg gtagcagtgg cgacgccaga
    27061 aatcggcgca ggcctggtag cgtagcagag actcatcttc aatcagctgc tgctcaaaca
    27121 gcataccgga ttcaaaggca ttcaggttga gcatgcgctg gcaaaaaccg tgaatagtaa
    27181 agactgccgc ttcatccatc tgccgttcgg ctaataacaa ccactgcgcg gcttgcgctt
    27241 tatcgtcgat ctcttccagc aggcgttcgt acagtggatt gtcggtggtt tcacgcagac
    27301 aggcgatgcg caactcgtgg atattgctac ggatacgacc gcgcaattct gccgtggcag
    27361 cctcggtaaa ggtgaccacc agcagttctt caacggtcag cgggcgggga aaggcggcgg
    27421 aaccgcctag tccaagtaac aggcgcaaat agagcgccgc aatcgtaaag gttttgcctg
    27481 tgccggcaga ggcttcaatc aggcgctcgc cctgtaaggg caagcgcaaa ggatctagtg
    27541 tctcggcgac atcactcatt cttttcactc atcaggggca ttgtttgctg caacgcgctg
    27601 acgttctccc acactttcca gccttcagga tgcacatatt cggctttccc gttctggctg
    27661 ccggaaatct gcgacaggat agccatgcct tgcggctcga ccaccgcctg atggaagaaa
    27721 tcagcaagtt tttgcggcgt cagcagtttt atctgggcca cgattttatc acgcgaatcg
    27781 aagcgcatat tgccgcgatc gaaatcttta cttaacttcg atgcttcttc gccgagcgtt
    27841 tgcggtgcct gcagcatctg ggtaattacc gcctgctgga tttgcgcaaa ctcatctggc
    27901 ttcatcgctc gcaattttgc ctctgcggtt gggaaaaacg ccttgtaacg ctcccacaag
    27961 aatgaaggct gtttatcatt gctttgcaaa aggaagccca tgccccactg acgccccacg
    28021 ctcattggaa acgcaaacac ggcatagccc aattgttctt cggtacgcaa ctgattgtag
    28081 aaccacggct gtacgatctg ccccaacaga gagctatagg ctgagctggt gtattcatcg
    28141 tagccagtcg gtacaaatac cgctgccagt gcggagtcgg tgctgttacc ggctttttca
    28201 aagatgacgg attgtttttt atcgaccact acatctttgt ttcgacacca ctctgaacca
    28261 tcagcgccca actgtttttg cacatcgcgt gccagcgttg ttgcctgggc ctcggtcatg
    28321 ttgccgataa ccataaactc tggtcgagcc cctgatttta aggcgtcgcg ataggccagc
    28381 acctctttca acgtaatgga gggcaaaatt ttacgccgtt catctcgcga gaagtacggc
    28441 acttgcgaga gcatctgcgc gggcataatc gcctgctcaa acgctttacc cttttctgcg
    28501 gaatccatca tctggttata ccaggacttc gcctgctcaa gctgatcttc cgtagcggta
    28561 tagctaaagt acccctcgag caatgcctgg aacagctgcg gcagacgctg ggtgtaacca
    28621 ttagcattaa ccataaggcc gttgttagcg ttggtggaaa aacttatgcc accaaccgac
    28681 gcctggttgc ttaactgatc aagcgccagc cctgcgagat aatcattgag cgcaaacatc
    28741 acctgattgc gggcgctgtc catggctttc ggattacgca aaatcaggct gacatcagct
    28801 ttgggttcgc tggaaaaata acggcttggc gcatacacca cgcgcagatt cgactcatca
    28861 acaatcagct ctggatggtc atatttcttc tctgacttaa tcagcgagaa atcgtcagga
    28921 atataggggt taagctctgg caaagagagc gcaatgtcgg cggctttttt ctgccagtcg
    28981 gcgaaagttt gtgcgctgat tttatcgacc tgatacggcg catcgacaaa gtaagccgtt
    29041 ttgttgtgcg gctctttcgg gctgatatac cagatacgcg cattctgcgg cgtcatcatc
    29101 gccagacgtt cctttactgc tttagcatcg taccgatcgg caatattgac tgcatccagc
    29161 gtatgctcaa caggaacgcg aatcatggta tctgccagcc attcgacgta atccatatca
    29221 cgggtgattg acggataacg gaagtcgata tccagcacat tcgccagttc atcgaagtat
    29281 tgtttatcga tccctttttc acgtaacaaa ttgaggtagc taaaaatagc cgccacaacc
    29341 tgatcgcgat tcgccagacc tttatcggtt aaagacgcag agatcgctaa tacgccgctg
    29401 ttgccgttga cgataggatc ggagttggcg ctaatgccct caactaatcc ctgcttttgc
    29461 agccagtcgg aaagtgtacc tgggctgcga ttaccaatca gataggtaat caattcatcg
    29521 gttttgctac ggaatttcgc cgagttgtta tcaatacgaa actctacgcg caacacttta
    29581 cgcggcagcg ccgggacgta atgaatgata atgccctttt gcgcgtcggt gactaccggc
    29641 acggtgattt ccggtttttt gctctctttg ttcggcacgc gaccaaaggt gtccgccgcc
    29701 atttttgcca actccggcag cggtttattg ctgtaaataa ccgccttcat cagattggcg
    29761 gaatagtact tctcgtggaa atctttcagc gcctgctgca ccggattacc aggtttgtcg
    29821 cttaaagttt cgaggttacc accagaaaac tttgaaccgg ggtgtgccgg gttaatggtt
    29881 tctgcgctga cctgtgccat gcgcatcccg tcacgcgtac gcgccatggt taattcagcg
    29941 ttcactgcat tacgctcacg ttcggcgtat ttcttgtcga gcaagggttc tgcaatagca
    30001 tcagccaggc ggtctaccgc accaggcaag gcgtcgttct caacttccag atagaaagcc
    30061 gtgcgatacg gcgcagtgct ggcattgtga ctaccgccgt gcattttgag atattcggcc
    30121 agactgtcag cctgcgggta ctttttcgac cccatcagac tcatatgttc aaggtaatgt
    30181 gccagcccct ggtacgcctc gggatcttcc agcgacccaa cgggcaccac cagcgccgag
    30241 agcgatttaa ctgcctgcgg atcagaaacc agcaagacca ccataccgtt atccagacgt
    30301 atagcctgat actggcggtt atctttatca cttttacgga tggtttcctg aatcggctgc
    30361 catcccgttt ctgcctgact taagggtgcc caaagggcaa ctaacaacaa taatgctttg
    30421 aaccaggtgc tgcggggcat tcacggacct cataatcaac ttaattttct gtccagattc
    30481 aacacgttaa cgcaaatcat ctgccagaat ttaatcttgt gctgcacgag tcagcctatg
    30541 tttatataac catcagtccg tgactggtgc gcatcataaa gtaagcggat aaattgcgca
    30601 atttttatac agcgctcatg actgattaaa gcgaaacagc ggtaacagga aacgttgcga
    30661 ctgttcaacg atggcctcca ttgtctctgg tgttaattgc cgccagagcc gttgatacca
    30721 gatatcatca ccttcgccac gcaccatcat gttgccttcg taagcctgca ggaatttcgt
    30781 acgggctttt tgcaacgtgg aatcgtcatc cagcatggca tcgttttgcg cgtcataaca
    30841 ggtttttagc cacgcgccgc cactttcagg taacaccagc aatggcgcgg acatcccttc
    30901 acgatacccc tcaatcagtt gtgagaggta atgcaaagcc tgttcggctg caagcggcgg
    30961 aaaacgccac tcgccgtctt tgcgtagaaa aaggcgactt tcaccattac caccgctggc
    31021 acagtagaca aggtgttcca gccaaagttg cattccctgc gccacactta ataaagaggg
    31081 acgccagcgc aacaagccat ccggctgcac ctgcggcaac cagccagtta tctgcacacc
    31141 gttgcaggcg agatcaattt ccatactctg ccccggctgg cgacaggcaa tgactctgtc
    31201 ggcaagctgc tgcatctcct ggcactgtgt ttcccagaaa atttcaccaa aagcgccata
    31261 cggtaaatcc cctgccgccc ggaagcggcg gaacaagcgt tcggcatcat cctgctcaac
    31321 cagtgcattc aataactgct gattgatttg ataacgacta agcccttcaa gaataaatgg
    31381 ctcggtgtcg gggatttcgc tgtcttcagt acggaagttc acctgcaaac gcatctggaa
    31441 gaacgcccgc accggatgtg cccagaatcg ttgtagcgtt tccagcggca cggtttccgg
    31501 taaggtaaac ggcagcggct gaacaaattc agaatgtgct ttaccagcct ggctggccgc
    31561 aggtagccat tcacgagcat agctttgtcg ttcgcctggc tggtagtttt gtggatcaaa
    31621 cggcatccgg gtatggaggc aagtaagatg cgcttttacc cttgcctcgc tttcatcaca
    31681 gttgagcgct tcatcgcccg gtagataatg actttgcccg atgtagtcga tcagttcctg
    31741 caccagtacc gacgggaaac gttcactgtt atcctgaatg gagcgcccga tatagctgat
    31801 ataaagtttt tgttgcgcgg aaattaacgc ttccaggaac agatagcggt catcatcgcg
    31861 acggctacgg tcgccacgct tcggtttctg gctcatcagg tcaaagccca atggcgcaag
    31921 ctgacgtgga taaacgccgt cgttcattcc cagcaggcaa accactttga acggaattga
    31981 acgcattggc atcagagtac aaatgttaac cggtccggcg agaaaacgct ggctgatacg
    32041 ttcttgatcc aggcgctgtg ccagttcatc acgcaatagt gacagcggca ccgcgtcgcc
    32101 atactgcgca cctaaacctt cggcgataat cgcctgccat tgttgttcga tcagcgtcat
    32161 cgccgcttcg gtttccgcat ccggcaggaa gaaggcgttg agcatatcgc gacaaaccgg
    32221 caaccactct tccagcggac gctcctgtgc cagcccgcga cgccagatgt ttagctgcat
    32281 tagcagggaa gccagatgcc ccaccagttc tgcaattaag ccgctcgatt catcataagg
    32341 tagaaccgat tgccactcgc cctgcgcgct ctccatcgcg taacccagca acatgcgcgt
    32401 caggccaaac cgccaggtgt gttgaccggt agcggggagt tccagctcgc gaacgttgtc
    32461 gtcatctatc ccccaacgaa tgccggattc gttgacccac tggcgtaaat aacgcagccc
    32521 ttcttcggtg atgtcaaacc gcgccgccag caccggcaca tccagcagcg ccagcacatc
    32581 ctctgacaca aagcgactgt caggcagtga taacaggctg ataaacgctt ccagtaccgg
    32641 atgtgactgc cgcgcacgac ggtcggaaat ggcgtaaggc aggtaacgat ccgcaggtgc
    32701 gctaccaaac actgcctgaa taaacggact gtagctgtcg atatcagcca ccatcacgat
    32761 gatgtcgcgc ggagtaagtg tcgggtcttc ctccagcatc gccagcaggc gatcgtgtaa
    32821 aacttcaact tcacgctgcg ggctatggca aacgtggaag gtgatactgc tatccagtgg
    32881 atcaagcggg cgtttgttat cgctacggga aaactcttcg atgttcacac cagcaacggc
    32941 gcggttttcc agttccagaa tgtcagactg aatgttatgc agcaggttat ctggcatcac
    33001 atcgacaaag gcgtccagct cctgactgct atccagatcg gaaaggagat agatatagtc
    33061 gcgccccaac ttgccccagg aggccagtag cgggttgccg acatcctgtt caccgtcgct
    33121 gttaaagagc tgtccggcat tttcgctatc gcgaaatagc ggcaattcgc gatcttcaaa
    33181 actgtgtcga cgctgacgag tcagcagttt cgccagataa gcaggatctt taatatcgcc
    33241 ccagtaataa cggcaggggt tggtaaacag gagatggatt tcaatatgtt tacccagcgc
    33301 ctggagcgcc tggagataaa caggcggtaa cgcggaaata ccgcatataa agacgcgcga
    33361 aggtaacccc ggcgggcagg tcgtcgcgga ctccagcgtt tcgataaagc gctgatagag
    33421 attggcgcgg tgccagcgcg gttgcccgag ttgatgggta tattccacca acgccttcca
    33481 caacggcgct tgccaggcct gtgcttctcc cagcccttca accaaatgtc ctgtttccca
    33541 ctgtgccagc cagtccggac gatagaccag atactggtca aacaggtccg ccgcttttga
    33601 agaaagctgg aacagttttc gcttgtcgct atcgtcagtc agataatgcc gcaacagggt
    33661 aaagtcttcg cgatccagca attgcggcag cagagtcatc agtttccagc tcatgctctg
    33721 tttgttaaag gcgctctctt tggggatttc cggtaacacc cggacgaaca tatcccagat
    33781 aaagctcgca ggcagcggaa aatcaatgtt tgccgcaata ccaaactttt gcgacagggt
    33841 catttgcagc cactgcgcca taccggtact ttgcaccaga atcatctctg gttcgaaagg
    33901 atcgtccagc cgttcgcgtt cgacaataaa ctccatcaac gcttccagca cgtccagacg
    33961 attggaatgg tagaccctta acatagcggc tcctgactac tgacgattcg ggcaatgcag
    34021 gcgggtcatc tcgccttctc tgcccccggg tgaaactagc gtaacgctga tgctgacaca
    34081 tcccgcctgc gatgtctgca ttcggttgac ctgccagttg gcaggtggcg aaatcgcgcg
    34141 cagttgcgtt tgctgccagc catgccgcca gagctgttgg tactggtttc tgctggcaag
    34201 actgttcatt aatgttcgct ggaaacccga taacgcagtg acaatcatca ccatcaacac
    34261 catcgccaac attacctccg gcaggctaaa gccttgttga ttcttcaggg aagctgacat
    34321 aacgccctct ctttcagcgg acaaaaatcg ctccagccgc gtggcgaaaa aacaatgttt
    34381 ccatcaatga cttcgcctgt tcgccacaac gaaacgcctt cataatcggc aatcaataag
    34441 gcttcattat ctgccagtaa acgcaaacaa acctgggcat cggttccagc gtactgcgag
    34501 cactgaactg ctggctgcgt ctgccaggag tgcatttttc cccacgccag cgccgactga
    34561 acgatggcct ggcggcgcaa tgactgactt tccatgctca cgcgagaggc aaaactgcga
    34621 tcctgttggc tcattccttg taatagcaag ctacctaaaa ccagcagcat caggaccaga
    34681 gccagtgacg aaacaccctt ttcgcggttc acaggttgaa tcctgtcacg ctatagctgg
    34741 cattcaccac ggtttgcggt tcagacttac tggcagcacg catattaacc gtcaacaccg
    34801 gcgagaagcc gctgacatcc tgatgtacga cctgaaaagt gtcgataatg atggcatccg
    34861 gattagtgac tttatcccag cccttacctt cacaggatgt cgcaccgcgt agcgtttcca
    34921 gcacatgctc cttcagacga aatccaatct ggtcagactc ttttaccggt tcgcgatccc
    34981 agataccgtt actgttcgca tcccactgca caatgataca gtcaccctgt ccgacaattt
    35041 ccagcccttc gccggtacag ctgccatgac aataacccgc cctctggaga tgcttcgcga
    35101 cggtaaatac ccgcagccag atttcatctt ccagcgccag cttacgggtg ctcgttaaac
    35161 tttcacgctg taacgcaggc agaaagcgtg cagcccccag caacaatacg ctactgatcg
    35221 ccatagcaat caacacttcc agcagagaaa aaccttgctc ttttacaggc atccttctgt
    35281 ttctccttgc tgacaaagcc ggagtcttcc ccacggcgaa accaccagcc accactcgcc
    35341 cgttgagttt ttgaagcgaa tatgcccggc ccatgcggta ttgcgcaggc caaagaaagc
    35401 aagcgaaggt gtcaggtcgc tcatttcgac ttcgggccag cgtggcacaa agaccaatgg
    35461 tgaactgcca tgacaggtat tggccccagc agcggaactc acaaggcacc ataacgtccc
    35521 ctccctgata acgctgatac tgtggtcgcg gttatgccag ttggcatctt cacgtaaata
    35581 gagcaaatag tcccgcgcct ggctggcggt ttgccatagc cgttgcgact gctgccagta
    35641 ttgccagcca tagagtccac ttgcgcttag catgaccaaa atcagcatcg cgaccagcgt
    35701 ttcaatcagc gtataaccac gttgtgtttt catgccggca gtatggagcg aggagaaaaa
    35761 aagacgaggg ccagtttcta tttcttcggc gcatcttccg gactatttac gccgttgcag
    35821 gacgttgcaa aatttcggga aggcgtctcg aagaatttaa cggagggtaa aaaaaccgac
    35881 gcacactggc gtcggctctg gcaggatgtt tcgtaattag atagccaccg gcgctttaat
    35941 gcccggatgt ggatcgtagc cttcaatctc aaagtcttcg aaacggtagt cgaagatgga
    36001 ttcgggttta cgtttgataa tcaacttcgg cagcggacgc ggttcgcggc ttaattgcag
    36061 atgagtttga tccatatgat tgctgtacag gtgcgtgtcg ccccccgtcc agacaaaatc
    36121 gcccacttcc agatcacact gctgcgccat catatgcacc agtaacgcgt agctggcaat
    36181 gttaaacggc aggccgagga aaacgtcaca ggagcgctga taaagctggc aagagagttt
    36241 gccgtctgcc acatagaact ggaagaacgc atggcacggt gccagcgcca ttttatccag
    36301 ttcgcctacg ttccacgctg aaacaataat gcggcgcgaa tccgggtcgt ttttcagctg
    36361 gttcagtacc gtagtgatct ggtcaatatg acgaccatct ggcgttggcc aggcgcgcca
    36421 ctgtttacca tacactggtc cgaggtcgcc gttttcatcg gcccattcgt cccagatggt
    36481 gacattgttt tcgtgtagat aagcaatgtt agtgtcgccc tgcagaaacc acagcagttc
    36541 gtggatgatg gaacgcaggt ggcaacgttt agttgtcacc agcgggaatc catcctgcag
    36601 gttaaaacgc atctgatgac caaaaatgga aagcgttccg gttccggtac ggtcgttttt
    36661 ctgtgtgcct tcgtcgagca ctttttgcat cagttctaaa tactgtttca tggttcctca
    36721 ggaaacgtgt tgctgtgggc tgcgacgata tgcccagacc atcatgatca cacccgcgac
    36781 aatcatcggg atggaaagaa tttgccccat gctgatgtac tgcacccagg caccggtaaa
    36841 ctgcgcgtcg ggctggcgga aaaactcaac aatgatgcga aacgcgccgt agccaatcag
    36901 gaacaagcca gagacagctc ccattgggcg cggtttacga atatacaggt tgaggataat
    36961 aaacagcacc acaccttcca gcagcagctc gtaaagctgt gatgggtggc gcggcagcac
    37021 accgtaagtg tcgaaaatgg attgccactg cgggttggtt tgcagcagca aaatatcttc
    37081 tgtacgggag cctgggaaca gcatggcaaa cgggaagttc gggtcaacgc ggccccacaa
    37141 ttcaccgtta ataaagttgc ccagacgccc ggcaccaaga ccaaacggaa tgagtggtgc
    37201 gataaaatca gaaacctgga agaaggaacg tttagtacgg cgggcaaaga taatcatcac
    37261 cacgataacg ccaatcaggc cgccgtggaa agacatgccg ccgtcccaga cacggaacag
    37321 atacagcgga tcggccataa actgcgggaa attgtagaac agaacataac caatacgtcc
    37381 cccgaggaag acgccgagga agcccgcata gagtaagttt tcaacttcat ttttggtcca
    37441 gccgctgccc ggacgattcg cccgtcgtgt tgccagccac attgcaaaaa tgaaacccac
    37501 cagatacatc aggccgtacc agtgaagcgc cacgggtcct attgagaaaa tgaccggatc
    37561 aaactccgga aaatgcagat agctactggt catctgtcac cacaagttct tgttatttcg
    37621 ctgaaagaga acagcgattg aaatgcgcgc cgcaggtttc aggcgctcca aaggtgcgaa
    37681 taatagcaca aggggacctg gctggttgcc ggataccgtt aaaagatatg tatatgatcc
    37741 gcgctataac cctccgcgaa tcagcccgcc catgccgcga cgctccataa aggctgcaac
    37801 ctgatggcga acttcggtcg ccagttgcgc ttccagacta cgctgcgcaa gattttctgc
    37861 ttcggcataa tcaatgcgcc gcagcaggta ttttacccgc gctacagaac gtccgttcat
    37921 agacagatgg cgatacccaa gcccaatgag gattgccacg cacatgggat cgcccgccat
    37981 ttcaccgcac aaacggagat cgattccatg tatttccgct tcccgggcga tcatcgccag
    38041 agctcgtaac attgcaggat gaagactgtc ataaatgttc gccacccggg tattgttgcg
    38101 atcaacggcc agaatgtatt gagtcagatc gttggtgcca acagagatga aatcgacccg
    38161 ctttgccaga tgcggcagca taaataccat tgacggcact tccagcatga tgccgatacg
    38221 tggtttggga atttcgtaac cgatcatctc ctcgacttca cgtccggcac gttcaatcag
    38281 gcggcgtgct tcatcaactt catcgaggct tgtgaccatc ggcaacagaa tattcaggtt
    38341 gcccgtagcg gcattagcac gcagcatcgc ccgcacctgg atcaagaaga tctccggctg
    38401 atcgagcgta atgcgaatcc cacgccaacc caggcatgga ttctcttcgc tgatcggcat
    38461 gtaaggcagc tgcttatctg ctccgacatc cagcgtacgc aaggtgacgg gtttatcatt
    38521 gaacatttgc agcatcccct gatactgcgc cacctgttct tcttccgacg ggaaaccact
    38581 ttgcagcatg aatgggattt cagtgcgata aagaccgatg ccatcaatac ggctgcccag
    38641 tttttcttca tgttccgggc ttaaaccagc attgagcatg acttttatac gctcaccgct
    38701 ttttaactgc gcgggtaaat tgacgtcatc ttccgccaga cggctaagct caatctcttc
    38761 actaattagc cgctgatatt cttgcagcag taccggctcc ggatcgacca gcaattcacc
    38821 gcgatagcca tcaacgatca gcgtcctacg atgcagcacc gaaggctgaa tatccgcgcc
    38881 catcacggta gggatcccca gcgcacgtac catgattgca gcatgggagt tggctgcgcc
    38941 atcgcgcacg acaacaccca ctaagcgatc ctggggcagc tcagcaagag ttgtcgctga
    39001 cagttcatct gccaccagaa tgaaacgttc cggccaggcg ttcggccctt gattagcgtc
    39061 atcaagatga aacagcaatc gctgacccag cgcacgtaaa tcgccagccc gctctttgag
    39121 atagttatcg cttagcgcgg caaactgttc ggcaaatttt tcaatgaccg tttttaccgc
    39181 ccactctgcc accgagcctt tatcaacctc ggcaaacaat tcgcgacgca gccgggtgtc
    39241 cgaaagcagg tgcgagtaaa gatcgaaaat agccgccgtt tctttttgtg ctccggcggc
    39301 aaagcgtttg ctgtagcggc gaaactcgtt tgccgcttct tccagcgccc cggtcagtcg
    39361 ttcgcgttcc agagccggat ccagcgttga tgcctgatac acctgttcca ttaaaggtaa
    39421 cgtgacatcc tgccagcctt cggcaatcgc cacaccaggt gctgccggta atgcgcggat
    39481 tcgcgtctgg cgatattgcc caaacaaggc agtcaactgc gactgagaaa gaatagctgc
    39541 catctgggtg gcaagcgtca ccaggaagga ttcttcactt tcgtcatact ggcgcaactc
    39601 tcgttgctgt accaccagta caccaagcaa ctggcgacgt tgaataattg gtacgcctaa
    39661 aaacgcgcgg aaacgttctt cttttacgga ggggatgtat ttgaagctgg ggtgcttttg
    39721 cgcatctgca aggtttatcg gttccgccag cctgccaacc aggccgacga tcccttcatc
    39781 aaacgcgagc gttacagtgc gaccgcgtgg ttttttcagc ccccgggtcg ccatcaggta
    39841 gtaacaacgt cgatcatgat cggcaaggta gaccgaacag acctcggtat ccatcgcaag
    39901 acagatgtcg gtaaccagaa tatttaacgc ctcattcagg cgtggtgcgc tggctacctt
    39961 ttcgactatt tcgcgcaggc gagtgagcat aattggcgtg acttaacctc ttttacgtcg
    40021 ataagcagat gcgttttgtg gttttggcgt attttcctgc agtgacatca ccacgctcgc
    40081 gaactctttc attaccctac ggtagacatc acgtttaaat gacaccacct gtctgaccgg
    40141 ataccagtaa cttacccatc gccagccgtc aaactctggt gtactgctgg tttgcatatt
    40201 gatttctgca tcgccgctca ccagctgcaa gagaaaccat ttttgttttt ggccgataca
    40261 aaccggcttc gtgtcccaac gcaccaaacg tttcggtaat ttgtagcgca accagttacg
    40321 cgttgaagca aggattcgaa cgtctttgcg gcttaatcct acttcttcaa acaattcacg
    40381 gtacatcgcc tgctctgcgg attctccggg gttgattccg ccttgcggaa attgccagga
    40441 gtgctgacca aatcgccggg cccacattac ctgcccctgg cgattacaaa tcacgatacc
    40501 tacgtttggg cggtagccat cgtcatcaat caccggacta cctcaaaata aagctttata
    40561 tacgaatgat tgtttcatac tccaggaaga cggtaaacca ctctctgcag ggcattacac
    40621 actaataaca attgaataac tcacagttat gtgcagagtt ataaacagag gaaggggtgg
    40681 atagccgttt ttattcacct tttctgtgga tagagttgtg aagaactacg gaattactac
    40741 gggaaaaccc ggagcattct gaataagccc gatatagaca ttttaaaata ttcattatta
    40801 gtcaatgcat tacatgtaat ttgagtaatg aaaaagagta attcgtgacc caggtcacac
    40861 ctctcattta cgggttggcg aaagatcaac caatgccgta tttatccaca gaatgtgcca
    40921 ctaagttaag cactgaacca ctaaaaactg gagtttcatc gcacgtcaag gctgtaaatg
    40981 gaaacagtag tggaggtttt tcacagttat cccagctttc tgtggataac atggtgtaag
    41041 atcctgttta ttttcagtga ccagatttgg aaaacctatt gcagtgttgc gcaactcgat
    41101 taccggcagc ctaaaaagca atataaatca gggaattgaa caacacatgt ggaaaaagtt
    41161 acactgcgaa tattcggcac ataattgctg tttgtttttt aatcaaggta tcatgacatg
    41221 tcccaacctc gcccactgct ctctcctccc gaaactgaag aacagttgtt agcgcaagca
    41281 cagcaacttt ctggttatac attgggagaa ctggcggcac ttgccgggct tgttacgcca
    41341 gagaatttaa aacgcgataa gggctggatt ggcgtgttac tggagatctg gctaggtgcc
    41401 agcgcaggga gtaaacctga gcaagatttt gccgctctgg gcgtggaact taaaactatc
    41461 cctgtggata gtcttggtcg tccgctggaa acaacattcg tttgtgttgc cccgttaacg
    41521 ggtaatagcg gggtgacctg ggaaaccagc catgtgcgcc acaagctcaa acgcgtactg
    41581 tggataccgg ttgaaggcga gcgcagcatc ccgctggcgc agcgtcgcgt aggatcaccg
    41641 ttgctgtgga gcccgaatga agaggaagac cgacagctac gcgaagactg ggaagaatta
    41701 atggatatga ttgttctcgg tcaggttgag cggatcaccg ctcgtcacgg ggaatattta
    41761 cagataagac cgaaagcagc gaatgcgaaa gcgcttaccg aagccattgg tgcccggggc
    41821 gaacggattc tgacgctgcc acgcggcttt tatttgaaga agaatttcac cagtgcacta
    41881 ctggcccgtc attttctgat ccagtagcca tcgctttgac ctgccgcttt ccgggcatat
    41941 aattaccgct tcattttttt ggcagggctt tttagatgtt atttgcatgg ataaccgatc
    42001 ctaacgcctg gcttgcgctc ggtacgctga cgctgctgga gatcgttctt gggatcgaca
    42061 atattatttt cctttctctg gtggtggcaa agcttcccac agcacaacgt gctcatgcgc
    42121 gccgtctggg gttggcggga gccatggtta tgcgtctggc gctgctggca tcaatcgcct
    42181 gggttacgcg cctgacaaat ccgcttttta caatattcag tcaggaaatt tctgcccgtg
    42241 atttgattct gcttctgggt ggcttgttcc ttatctggaa agccagcaag gaaatccacg
    42301 aatccattga aggtgaagaa gaagggctga aaacacgcgt ttcatcattc ctcggcgcta
    42361 tcgtgcagat tatgctgctg gatattatct ttagcctcga ctcggtgatt acggctgtgg
    42421 gtctgtcaga tcacctgttt attatgatgg cagccgtggt gattgccgta ggcgtgatga
    42481 tgttcgccgc gcgctcgatt ggtgattttg tcgaacgcca tccttcggta aaaatgctgg
    42541 cgctctcttt cctgattctg gtgggcttta ccctgattct ggaaagtttc gacatccacg
    42601 taccgaaagg ttacatctac ttcgcgatgt tcttctctat tgcggttgaa agcctcaact
    42661 tgattcgcaa caaaaagaat ccgctctgat actccgtacg ctctcctgcg ggagggcgta
    42721 ctcactcctt ttaccctcac agtttaagat tttcctgctt tcaagatata taacgtcggt
    42781 ttataaacag actattatca taggtaggcc aaatgaggat aaccggatga aaaaatgggc
    42841 agtaataatt tctgcagtcg gactggcgtt tgctgtttcc gggtgttcca gtgattacgt
    42901 catggcgacc aaagatggcc gtatgatttt gaccgatgga aaacctgaaa ttgatgatga
    42961 taccgggctg gtgagttatc acgatcagca aggtaacgcg atgcaaatta accgtgatga
    43021 tgtttcgcaa attattgaac gttaacaaat aaggtcagca tccggctggc cttaagattt
    43081 ttctctttcc cttttccctt ccctctgcca tttttatatt ccttatgtcg tgattataaa
    43141 aaggaaacgg ctatgcaata tcaccgtata ccccacagtt cgctggaagt cagcacgctg
    43201 gggcttggca cgatgacgtt tggtgaacag aacagcgaag ccgacgccca cgcacaactc
    43261 gactatgccg tcgctcaggg cattaacctt atcgacgttg ccgaaatgta cccagtacct
    43321 ccgcgccccg aaacgcaagg gttaaccgaa acctacgtcg gcaactggct ggcgaaacat
    43381 ggcagccgcg aaaagttaat tatcgcctcc aaagtgagcg gaccgtcgcg caataatgac
    43441 aagggcatcc gcccggatca ggcgctggat cggaagaata tccgcgaagc gctgcatgac
    43501 agcctcaagc gcctacagac tgattacctc gatctttatc aggtgcactg gccgcagcgc
    43561 ccgaccaact gcttcggcaa actcggttat agctggacgg attctgcgcc tgcggtttcg
    43621 ctgctggata cgctggacgc actggcagag taccaacgcg cgggaaaaat tcgttatatc
    43681 ggcgtgtcga acgaaactgc atttggcgta atgcgctacc tgcatctggc ggacaaacac
    43741 gatctgccgc gtattgtcac cattcagaac ccttacagtc tgttaaaccg cagttttgaa
    43801 gtaggtctgg cagaagtcag ccagtatgaa ggggtcgaac tgctggccta ttcgtgcctg
    43861 ggtttcggca cgctgaccgg gaaatatctc aatggtgcaa aacccgctgg cgcacgtaat
    43921 acgctcttta gtcggttcac ccgctatagc ggtgagcaaa cgcaaaaagc cgtcgcggcg
    43981 tatgttgata tcgccagacg tcatggcctg gaccctgctc agatggcgct cgcgtttgta
    44041 cgccgtcaac cgtttgttgc cagcactctg ctgggcgcaa ccacgatgga tcagctgaaa
    44101 actaacatcg aaagtttgca tctggagtta agcgaagacg tattagctga aattgaagcg
    44161 gtgcatcagg tttatactta tccggcacca taagattaac gccggttaaa accggcgtta
    44221 aatattaatg acggcgctgc cagatccaca gcgccgttat tgccagcgca aacagcgcac
    44281 cgaagccaat gccaatgggc acgaccggga tgcctatcat taccgccagc gagtaaatgc
    44341 ccagcatcaa caacatggcg ctgttttcgc caaggttttg tactgcaatc gcattccccg
    44401 ccccgacgct ttttttaccc cgctcctgta gcaacgcatt gagcggaacg acaaaaaagc
    44461 cccccatcac gccaatcagc atcaacaagg cataggctgg cagcagctcg tgttgcaggg
    44521 aaaaaatcag taccaccacg ccaatcaaaa tcccggctgg catacagcgt gacacggttt
    44581 ccagcgtaac taacttcgct gccgcacctg cgccaaccac gataccaatc gctaccatcg
    44641 cgttgagata ggtgggcgta gcgttatcgg taatgcccag cgccaccggt acccacagca
    44701 ccaacaggaa acgcagcgtg acacccgctc cccagaataa actggtgccc accagcgaaa
    44761 aacgcgtttc accattgcgc catagcgagg tgcaggcatt caggaaactg cgggtcatgt
    44821 taatgagatt ccaggactgc cccggacgcg ccgccgccag tttgggaatg tagatattgg
    44881 caacgaccgc accaccgtag gccagtgcgc atgcggccag ggcgacgagg acatgccagt
    44941 cagccagcac accaccggct acggaaccga gcaaaatcgc cgctatggta gaagcttcca
    45001 ttaaaccgtt agctttcact aacttactac ccgtggttaa ttcgccgaga ataccgtatt
    45061 tcgccggtga ataggctgca gcaccaacac ccaccagcgt atagccgaga aacggattga
    45121 taccaaagca gatactggct gcgcccagca gcttcaggcc gttggcaaac atcatcaccc
    45181 ggcctttggc gaagctatcc gccacctgcc cgacaaacgg cgcaaaaaga atgtaagcac
    45241 ctacaaacac catttgcagg atgggctggc tccactccgg atagaactgc gctttcagta
    45301 acgccagagt ggcaaacagt agggcattat cgccaaacgc agagagaaac tgcgccacga
    45361 taaccgcttt catccccttc gaccacaacg aagtgttagt gtgcactgac tcactcatcg
    45421 tgttgttccg cttcgtctac ccagcttttc aacgtgacaa agtcaggttt gccgctgcca
    45481 agtaatggca tctgtttcag atagcgaata tcgcgcggta cagcaagctc cggcacgccg
    45541 tgctcgcggg catactgttg caacttatcg cgcgtcagtt cgttatctgt ggtgaaaagc
    45601 accagtgcct cgcctttgct ggcatcgctc ttaatcgcag tggcatggac tttatctggc
    45661 gaaacaccaa gtgccagttg ttccaccatt tccagcgaca ccatttcgcc tgcaattttg
    45721 gcaaagcgtt ttgcgcggcc ctgaatctgc acaaagccct gctcgtcaaa acgcacaata
    45781 tcgccagtgt cataccagcc gcgctccatt tcgccgcgaa cattctcggc ggtgggcact
    45841 tccagtacac ctggcttctc cacccgcaga tagccgttca ttatgttcgg ccctttcagt
    45901 tgcaggcgtc cgccctcttc gataccaggg accgacaaca ggcgcgcatc cattcctggt
    45961 agaatacgcc ctaccgtacc gggtttcgcc gccatcggta cgttgataga aacgacaggc
    46021 gcgcattcgg tcacgccgta gccttcaagg atgcgcaggc caaatttatc ctgccaaagc
    46081 tgtttggtac tttcttgtaa tttttctgcg cctgccacca catagcgtag acgatagaag
    46141 tcatacgggt tggcgaaacg cgcgtagtga ccgaggaaag tcgaggtgcc gaacaacacg
    46201 gtgcaactgc ggtcatacac caactccggc acaatgcggt aatgcagcgg gcttggataa
    46261 aggaacactt ctgcacctgt aagcagtggc gtaaacaggc ctaccgtcag cccaaaggag
    46321 tgaaacagcg gtaacgccga cataaagcga tcgttggtgg tgaagtcggc aatcgtttta
    46381 atctgctcga cattcgccag aatgctttta tggctatgga cgacgccttt cggatggcct
    46441 tcagaaccgg aggtaaagag gatcagcgcc tcttcttccg gctgctgttt aacctgtgcc
    46501 agacgcggca tcagcaaatg agcgaagatc catactttgt cggcagtggt gacatctgct
    46561 tttaaatctt ccagatagac ccagcgcacc tgagtaagtt gctccggcag atgccagagt
    46621 ttgcctttat cgagaaactg gcgggaagtg aagatggttt tgatttcagc cgccgtaata
    46681 gcactggtca gcccttttac cccggcagtg tagttcatca ttgcgggcat gcggcgacgg
    46741 gcgatggccc caaaaatcac tgccgcactg atgcctgcat tgggcagcat taagccgatg
    46801 cgttcgcctt caacactgta tttttcaagg atgcgtccaa caaacagcgt tttcgtaagc
    46861 aatttgcgat aggagtctgg ggtaaagttg acgtcttcga cacatttctt cccggctccg
    46921 aagcggtaca ttgcactcag taaagattcg tacagcgttt cacgcgggcg caccgccatt
    46981 cgcgcttcca tcattatttg atgcagcatt tcgccagcga ttttgcgacg gtcacgggca
    47041 cgcggcgcat ccggcatcgc cacctgcgtt ggtggcaaaa tatgcagagt aatttgcggg
    47101 aacaagcgac gtttaaccag acctttcagg cggctgaagt gcgtaagttc cgccccttca
    47161 atacgcacag gaataaccgt tgcaccagac ttcgccgcga caaaacccgc gccatcgtag
    47221 attttcatca gcgagcctgt agtggtgatg cgtccttcag ggaaaatcac cactggtcgg
    47281 ccctgttcca ccagacgtac cagatgttta atagccatag gttgcgtcgg gtcgagagga
    47341 acaaagtcga taaatgattt cagccaacgc atataccact gttggcttat tgaggtgtaa
    47401 acggcaaaca ctggacgcac aggtaaaaac agtccaagca aaatgccatc aataaaagag
    47461 acgtgattag gcgtaattag aacgcgctcg cccttcagtg cctgggggtc acccgtaacg
    47521 cgaacgcgat acaaaacacg gcacaaattt cgaaaaaagc taaaaagcat tccaactccc
    47581 tttgctctga ttcagtaaaa gcgaatggag ggagattaca cgagataaag aacgcgagcg
    47641 acagtaaatt aggtgcgaaa aaaaacctgc gcatccgcgc aggttggtgc aagagacagg
    47701 gtacgaagag cgtaccgaat aatctcacca atcaatacct ctgggatctt gattgtggtc
    47761 tgcacgacga ctcttcgcca gcgagaaaac gcaaaggaat gaagggaaat gcaacgaggt
    47821 gtgtaaattg tcggttactg ttacagattg atgaccggca aaaaaaaacc tgcgcatctg
    47881 cgcaggctgg tgtaattcat gtgctcaacc cgaagttgac ttcacctatc aatacctctg
    47941 ggatcaccac tttagcaacc tgaagccaaa cgccaccagc ggtcaatcgc aacagcgttt
    48001 cgcaaagtgt aaccaaaggt ttgcaatctc tttttttgtc ttacttattt gcgtttggct
    48061 cacattccca cgatgaaaac acgccacccc ttgaaccaac gggcgttttc cgtaacactg
    48121 aaagaatgta agcgtttacc cactaaggta ttttcatggc gaccataaag gatgtagccc
    48181 gactggcagg cgtttcagtc gccaccgttt cccgcgtcat taataattca cctaaagcca
    48241 gtgaagcttc ccggctggct gtgcatagtg caatggagtc tcttagctat cacccgaacg
    48301 ccaacgcccg tgcgctggcg cagcagacca ctgaaacggt cggtctgatc gttggtgatg
    48361 tttccgatcc gtttttcggt gcaatggtga aagcggtcga acaggtggct tatcacaccg
    48421 gtaatttctt attgattggc aacggttacc acaacgaaca aaaagagcgt caggctattg
    48481 agcaactgat ccgccatcgc tgtgctgcgt tggtcgtcca tgccaaaatg atcccggatg
    48541 ccgatttagc ctcattaatg aaacaaatgc ccggtatggt gctgatcaac cgtatcctgc
    48601 ctggctttga aaaccgttgt attgctcttg acgatcgtta cggtgcctgg ctggcaacgc
    48661 gtcatttaat tcagcaaggt catacccgca ttggttatct gtgctctaac cactctattt
    48721 ctgacgccga agatcgtctg caagggtatt acgatgccct tgctgaaagt ggtattgcgg
    48781 ccaatgaccg gctggtgaca tttggcgaac cagacgaaag cggcggcgaa caggcaatga
    48841 ccgagctttt gggacgagga agaaatttca ctgcggtagc ctgttataac gattcaatgg
    48901 cggcgggtgc gatgggcgtt ctcaatgata atggtattga tgtaccgggt gagatttcgt
    48961 taattggctt tgatgatgtg ctggtgtcac gctatgtgcg tccgcgcctg accaccgtgc
    49021 gttacccaat cgtgacgatg gcgacccaag ctgccgaact ggctttggcg ctggcggata
    49081 atcgccctct cccggaaatc actaatgtct ttagtccgac gctggtacgt cgtcattcag
    49141 tgtcaactcc gtcgctggag gcaagtcatc atgcaaccag cgactaaccg cagttaaagc
    49201 aattccagcg ccagtaattc ttcgatggtc tggcgacggc gaatcaaccg cgcctgacca
    49261 ttatcaaaca gaacttctgg taacagcgga cggctattgt agttggatga cattgatgcg
    49321 ccatatgccc ctgtatcatg cagtaccaga taatcacccg ccttcacttc cggcaaggcg
    49381 cgggtttcaa catttccccc ttcctgctgg gtaaagacat cgcccgattc acataatggc
    49441 ccggcgacga cggtttccac cgttggcgcg tgttccagag aacgaccatc agctgccagg
    49501 gcactgatat ggtggtaact accgtacatt gccgggcgca tcagatcgtt gaacccgaca
    49561 tcaaccagca caaagtggcg gctacccatt tgtttgacgc tccgcacctg agtaattaac
    49621 acgccagact gcgctaccag aaagcgaccc ggttcaattt ccagtttcac agggtggccc
    49681 aaatggcgag cgatttgctc acgcgcggca ttccacagac cataataatg ttcggtatca
    49741 accgcctctt caccctgttg ataaggaata gaaagcccac cgcccgcaga aatagcctgt
    49801 aaatcctgac cgaattcgat gacctgacgt accatagcac cacacacctg ttccagatgg
    49861 gcataatcaa cgccagaacc aatgtgcatg tgaatgccga ccagctgcaa atgatgacgt
    49921 tgtatcacgt ccagtgcggc gggcagatcg gtgtaccaga taccgtgctt gctgttttcg
    49981 ccaccggtat tggttttttg gctatgtccg tgaccaaacc ccgggttaac gcgcagccat
    50041 acccgatgcc ctggcgaaac ctggcccagt tggtcgagca tatcaacaga acccgcattc
    50101 accggaattt gcaattcact gacgcgttca agcgtcgcct gatcgataac atctgccgta
    50161 aaaacaatat catcggggtg cgtttgcgga ttgtaacccg ccgccaacgc acgctctatt
    50221 tcgcctaacg agacggaatc cactttcacg ccctgctcac gcattaagcg caaaatatga
    50281 atattggaac aggctttctg tgcaaagcgc accacatcaa actgtttcag cgctgcaatc
    50341 tgccgacgaa taatttgcgc atcgtagacc cacaccgggc agccaaattc agcgggcaaa
    50401 cgcagcagat tttcggcggt gagatcggta tcggtgctga acagtgaatg tggcataaca
    50461 aactccagat aagtgctttt ttatgattac gccacatcat aaaaagaata aaaaatatcg
    50521 atttatgtcg agtctatgca aaaatgatat ggattaccgg attgcgagag agcgctaatg
    50581 gccgccgtta acttacgtca tattgaaatt tttcatgcgg taatgaccgc cggaagcctg
    50641 actgaggcag cacacctgct acacacctca cagccaaccg tcagccgcga actggcgcgc
    50701 tttgagaagg tgatcgggct aaaattgttt gagcgcgtac gtggacgatt acatcctacc
    50761 gtgcaaggac tgcgtctgtt tgaagaagtg caacgatcct ggtacggact ggatcgcatt
    50821 gtcagcgccg cagaaagtct gcgcgagttt cgccagggag aactgtctat tgcctgcctg
    50881 ccggtctttt cgcaatcttt tttaccgcag ctcctgcaac cctttctggc acgttatccc
    50941 gatgtcagct taaatatcgt gccccaggaa tcaccgctac ttgaagagtg gctctcggcc
    51001 cagcgtcatg atttaggact cactgagaca ctccatacgc ctgcgggaac agaacgtacc
    51061 gaattactct ctttagatga agtgtgtgtg ttacctccgg gtcatccgct ggcggtaaaa
    51121 aaggtattaa cgccggatga ttttcagggt gagaactaca tcagcctttc ccgtactgac
    51181 agctatcgcc agttgctgga tcagctattt actgaacatc aggttaaacg acgcatgatc
    51241 gtagaaaccc acagcgccgc gtcagtctgc gcaatggtac gggcgggggt aggtgtttcg
    51301 gtggttaacc cgctcaccgc actggattat gcggcaagcg gtttagtggt gcggcggttc
    51361 agtattgcgg ttccgttcac cgtcagcctg atccgccccc tgcaccgccc gtcatcagcg
    51421 ctggtgcagg cgtttagtga gcatttacaa gcggggttac cgaaactggt cacttctctt
    51481 gacgctattt tgtcgtcagc tacgacagca taaaagcgac agcatcctcg gcatggatcg
    51541 ccgcagtatc aaacacaggc aaaacactgc gctcttctgg caccagtaaa ccaatttctg
    51601 tgcagccaaa aatgacgccc tgtgcgccct gttctgcaag gcgagcaatc acttgcgcat
    51661 aataagcgcg tgacgcttcg gtaaattgcc ccagacacag ttcttcaaaa ataatctgat
    51721 taattttcgc ccgttcatcc gcttcaggaa taagacaatt gatggaaaat tgttccgtca
    51781 gccgcccgcg ataaaaatcc tgttccatgg tgtaacgcgt acccagcagc gccacacgag
    51841 tcattccggc cccggtaatt gcacgtccgg tggcatccgc aatgtgtaag aaaggcagag
    51901 tgcaacgtga ctcaatggca tccgccactt tatgcatcgt attggtacat aacacaatac
    51961 cttctgcgcc cgcccgctgt aagccaagcg ccgcctcagc cagaatgtcc ccggttttat
    52021 cccattcccc gcgacgctgg cactcttcta tttcatgaaa atcgacgcta tgtagcagca
    52081 cttgcgcaga gtgaagccca ccaagccgct gtttaatgcc ttcatttatc agacgatagt
    52141 aaggaatggt ggattcccag ctcattcctc ccagcaaacc aattgttttc attcctctct
    52201 cctgatgtgt gttaccgcaa caaggctgaa taaggaacga gacaaacgcc tcaacggcca
    52261 ggtgcccgat ctctattaac gaaaaaaggg ccggatgtac agcacatccg gcccgtgaaa
    52321 tcagacgccg atatttctca acttctcgcc tgccatcagt ttgcgttcga tatgttccag
    52381 cgtgacattt ttggtttccg gaatgagcca gaaagtgatg cccacaaacg caatgttcag
    52441 cgcagtgtag agccagaacg taccggcagc gccaatgcta tcaagcagtg tcaggaaggt
    52501 cgcgccgata atcatattcg acacccagtt tgtcgtcgtc gaacaggtaa taccgaaatc
    52561 gcggcatttc agcggctgaa tttcagagca caggatccac accactggcg cggcgctcat
    52621 cgcataaccg gcaatacaca tcatcgtcat gccaacagag agccaggaca agccactgga
    52681 agccgtaccg ttatcaaact gcatcaggca atagcccagc accagagtgc ctaacgccat
    52741 cacgctgaaa ccaattttca gagccggttt acgacctgct ttatctaccg taaagaccgc
    52801 aatgaaggtc gcgaacataa aggtcagtcc cacgaccaga gtcgcaatca tctgttgttc
    52861 tgtggtcgta aagcccgcca ttttgaagat acgcggcgca tagtacatga tgatgttcat
    52921 accggtaaac tgctgcatcg cctgcaacaa cataccgaga aacacagcac gacggacgtt
    52981 acggttgatc ttaaacagtg cccaaccgcc ttgttttaac ttcaggcttt cacgaatttc
    53041 gttgagttct tctcgcgctt tttccgacgt atcgcgcagc atacgcaaca cttcttccgc
    53101 ctcaatatga cgcccctttt ccgccagcca gcgcgggcta tttggcagga agaccaccag
    53161 aataatcagc aggactgctg gtaaagcaag aacccccaac attgcgcgcc agttaccgct
    53221 ataactgaac gctgtatcgg ataaaaacgc cagcacgatg ccgagtgtga ccatcaactg
    53281 gtacatactg atcatcttac cgcgaacgtt ttcacttgcc atttcagaaa gatacagagg
    53341 agcggtgtaa gacgcgatcc cgacagcaat gcccagcacc acacgagcgg cgattaacat
    53401 ctctacgctg gtcgcaaaag cggaccctat agaaccgagt acaaacagga tggcccccgc
    53461 catcaggctg tatttacgac ccaggcggaa cgacagccaa ccattaaaca gcgcaccaat
    53521 tgctgcaccg agcatcatgc tactgaccac ccattcctgc aaacggctgg tcagcacaaa
    53581 gtgatcggta atgaacggca acgctccggc gattacgccg atatcaagac caaataacaa
    53641 tcctgcgacc gcagcagcta ccgaaacaaa catattcata cgccgcgtat cccgcaaaga
    53701 acgtggcgtt aaagcagatt ccgtattgat agtaaccatt ttttcctgcc agcagagagt
    53761 aagacatagt gaaaaaatac gtgaacaatt cacacaggtg tcaggtcgga aacagcataa
    53821 atatggatta aattgctgcg gcatgtcgtt atgtgatgga tattccaatt ttcaaattaa
    53881 gttgaattat tgagattatt attaacca
//
LOCUS       DAZFQD010000014        49800 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.014, whole genome shotgun sequence.
ACCESSION   DAZFQD010000014 DAZFQD010000000
VERSION     DAZFQD010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 49800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49800
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.014"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..812
                     /locus_tag="ACHKTW_000943"
     CDS             <1..812
                     /locus_tag="ACHKTW_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001165562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DotH/IcmK family type IV secretion protein"
                     /protein_id="HFO6462011.1"
                     /translation="TGPLPPPASPLVQEAASLDSPLNAGEIRELRGRMADNERAISAP
                     VTSVVPRISSLTVNLSPGASLPLVRTALHNVSVVTFTDINGSPWPQSDPPYNAAPGHF
                     DVQFNGNMVSVTPLRAWAAGNVTVYLKGLSVPVILNITSGETDTNTLSQEIDSRLDLR
                     IPRQGPVSPVVAVPSEKIALHDATLQAFLDGVPPDDPTVRRLKFSGNVPDTTIWQHGD
                     DLLVRSRAMLRDEFEQTLASADGTRLWKLPVTPLLTFSVNGQSIHVTPELE"
     gene            816..2147
                     /gene="traO"
                     /locus_tag="ACHKTW_000944"
     CDS             816..2147
                     /gene="traO"
                     /locus_tag="ACHKTW_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001272008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraO"
                     /protein_id="HFO6462012.1"
                     /translation="MSAEQDTAAKSGKKLSALLVLGGVIIIGGGYLLLTWLSPENSAS
                     LSSVNINSTTTGSGRTVVESQHYRDLLRADNERGAAEALRNNQTFIASLPRGLDTDHG
                     KNQSRDTQPADQAEGSGQPSTPPQADQTAREKQREALQKLLARINTQHPDARPPVVAA
                     AMWGTTGAPGISSHASPPGTGTSPQPRLQEASLATPAARTGIQLIPALTRVPAFIDTE
                     VDSDNAVSRVVASVPAGPWAGAVLYSQDARLAGKGMDIHFDRMMWRGMFLKVNAYALN
                     EHTGMSSVASDVNHRWFKHIVLPSVLGGVGSVGSLYKDANTQVIQGNYGSITGRVGMP
                     DGEALAGVIAGGMAERGSQLLTRQAESEPYRQVTVSRGEVVSILFVEPVMSNDISPPS
                     SGTAPSVPPPSGRPLTQEEAERQASDRIQAAVMRKQNEMRQRYGIQQESHP"
     gene            2144..2857
                     /gene="traP"
                     /locus_tag="ACHKTW_000945"
     CDS             2144..2857
                     /gene="traP"
                     /locus_tag="ACHKTW_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000787010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraP"
                     /protein_id="HFO6462013.1"
                     /translation="MKNEHEDPGSFGETTTPASGKSFLKRQYMGYSLPVWAGCVLIIL
                     AAVWYKFLRTPSEPPADAGFAVTPPAAESWAEPMPMTETGSGNTMSAPPVAPQADMQQ
                     LARDLKAELDRRDEEIRRTLTMLKESITTLGDTIRKDEAWAQETRQLLDETRKQVHAL
                     QMQGPLQIPTGNNGQSTKTPPSRLQGMKIMSVEKGMAWIRWQGSAWAVREGDRLGNIT
                     ITRIEPSSRSVITSAGTLR"
     gene            2854..3384
                     /gene="traQ"
                     /locus_tag="ACHKTW_000946"
     CDS             2854..3384
                     /gene="traQ"
                     /locus_tag="ACHKTW_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000987018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraQ"
                     /protein_id="HFO6462014.1"
                     /translation="MMSIDALTAIENFASGIFSAGMEFLFTWGELLGIIGLIGHLMRA
                     RAEGRHSMGPGKFIAGIVICGMLVALPSFINAGGTQMGFRADSFGPIAYVQPTTFGAA
                     AGAANAMLSLVKLAGVGFAMNGISIWRKSLLDGHTALSSSESISKGNVKFVAGVLLVF
                     IDRVIDASLASLGLAF"
     gene            3431..3829
                     /locus_tag="ACHKTW_000947"
     CDS             3431..3829
                     /locus_tag="ACHKTW_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001605438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6750 family protein"
                     /protein_id="HFO6462015.1"
                     /translation="MSRFRAMAARLCAAFIYVQLCLVACRDRLIRLLLLLPVILLPHT
                     AMANTLFDMIKTVGDGAKGSSSSFVAIGQVIGLVLFIGGLLGLKQIGKKPGAGLACLL
                     SILVGLCLAVVPELMGSGQKTLGLSPVSIS"
     gene            3886..4137
                     /locus_tag="ACHKTW_000948"
     CDS             3886..4137
                     /locus_tag="ACHKTW_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003665769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraS"
                     /protein_id="HFO6462016.1"
                     /translation="MSCELPPFTLLRTEHGNVIARDNRDNQEFVFAECYSTAIAALIL
                     AAVTLYTERKTYEQNTATHRPGPGTLAFPAPATGHGIQR"
     gene            4052..4870
                     /locus_tag="ACHKTW_000949"
     CDS             4052..4870
                     /locus_tag="ACHKTW_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001353682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraT"
                     /protein_id="HFO6462017.1"
                     /translation="MNKTPPLIAPVRERWLFPLPQPDTVFSGESLLPPPVLSTPGRCP
                     CCRRTVTHRFILEDSWPLQQMADTCRDTVVLLEKNLTRVMRLKKHPVPENADEKKKHT
                     RTLQDAERSLAQARLSARRLALRHVEKSQIVTTDALSENESELLQPEGPPFHLCAFCH
                     AWHCLNGYAAAQGVMVWLPDLHPASVVALNARALKEIFSDERKRVRQGRAVLNALVQN
                     RLAVEEKFRTWRPADFADALRRWPPAQRKTLREKMDGVALILLPDSFPDKKYVM"
     gene            4929..5288
                     /locus_tag="ACHKTW_000950"
     CDS             4929..5288
                     /locus_tag="ACHKTW_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6462018.1"
                     /translation="MDTHSAGYQFTADSFRRFIIRTLTDNIPPDGDEKEIARFILRHP
                     AFNGGWLLFLAAEIHERSRTHKSITVTVSVDNYLSWMDIAVSGLPELPDMPFCDFFIR
                     RQQFIYPGVPPTKSGEE"
     gene            5288..5668
                     /locus_tag="ACHKTW_000951"
     CDS             5288..5668
                     /locus_tag="ACHKTW_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6462019.1"
                     /translation="MKIHRISPETLITLIHAHLAGKTDSTAKEEHRLLRRFLRDDDGR
                     LAGVLLNIAGILQFNRELSARHNYPATPLTEFSLRKRGKQLHLCLCSLRFFYIPPVFI
                     QNKRRKSIVVHLNKITYKQTHSIR"
     gene            complement(5641..6186)
                     /locus_tag="ACHKTW_000952"
     CDS             complement(5641..6186)
                     /locus_tag="ACHKTW_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001390888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6462020.1"
                     /translation="MKAENKILNLFHATTMSRGLQIIKDGKIRTDAPSVICWSLETTP
                     GWIYLSDQLDNAVHWGNKIACLSKDELFFYIFRVQVQEENCYPDTDDLQYVSWLSPEE
                     AENTDLYTCLSLCHSCRTDKELYTGCEISDYAVLPATTLYTDNSGHPQYDIVNTLKKH
                     IGKDEYNQYESLRQRIEWVCL"
     gene            6411..9455
                     /locus_tag="ACHKTW_000953"
     CDS             6411..9455
                     /locus_tag="ACHKTW_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFO6462021.1"
                     /translation="MNINRIIFAIEDCISTLSRYSVSAFFPDYCDLHTVIGLDQQDRE
                     RRPWLKAPYIATTKQGYYLSVFEISGALKEMDENPDLTAPGTLESLITALAERMNTAY
                     KNTGHKISFVFERDPERGRDEIEAMLLPQRKSIARTGILLQDVLDEKVTTLTPWLVRE
                     RCWLTVWSSEELLSRTDRKDHQTRIRKLAEHAPPARFAQDPWRWTLSALKIRHDAFLD
                     TLEQALTHDSDGLLVRLMDIHEVGREIRRQLERNSTPAVWQPHLPEDARPAGWRQGED
                     TSVFHAPSLNLQLFTTQPETHGSLIKAGDLWHGMVAITLPPQNLRTFNQLVRDVPHAI
                     PWRIRMDLMPGGMKALGVKKTLLTYSSFISPLRPMYDSVMMLNDINKHDPVCIMTIVA
                     TTWGNSREVCTRNQALLKSALEGWGVCGTTTTFGDPRRAWVNTVLAASHGSGPVPLYP
                     PLSHALSLFPLNRAGSVWRGQGNLMMHTEDGSAWEVALASSQQNKHTELTPGAPGLGK
                     SVLINALSEIQIASAQKNLPFIAYIDKGFSAQGLVQLIRDSLPEQRKDEAVGIILSND
                     PEHTRNLFDVMYGAKKPATPEKNFMVSVLCALCVDTGTGQPCNPGDTRQIISNLVDLA
                     FREYGENNPRLYRAGTEPLVDLALEESGIAEQHDAGWWNAATWFEIRDMLHMAGNIPA
                     AQRAHYQAMPLLAEMSALLGQPSIRDVFGTVQRDNSAELLLDYIRRALDQGHSDYPMM
                     SGCTRFMLSPDTRVVAVDLNNVAGDKTPAGRLRTGIMYLLAGQIAGGDFVLPQYQEEI
                     RKNLDPRYHEVIFRRIEQLDQEVKTKVYDELHNAKGINFIWEALDTQELEQRKFGIRT
                     VLSTQLLQDYPPAVLKSANTLWLLRYRPDEIPFLRDNFGVPEVTLRRFLKMPEGAAPD
                     GSGVPVLAVFRVKNGTLARILKFTLGPLELWALNSSPKDSALRRALTQELGSLRARQI
                     LAENFPRGSATSLIEHRARTHDSENVIHDLAAELIRKQGYHL"
     gene            9455..10072
                     /locus_tag="ACHKTW_000954"
     CDS             9455..10072
                     /locus_tag="ACHKTW_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraV"
                     /protein_id="HFO6462022.1"
                     /translation="MYSIHITPRCRRPARGQVFLKTLRVDGDSFSFTLPSACFTLRPS
                     PNPRVIFASDRRADGEMSGVRLPLPGETGTLITTATWQVNVPERPPFDIQHVIHWLLP
                     SPLTALMSDNTWLWPDSPGTPALTAGDWPVMETDTPTLKRVKRRTFTLIDNRNRQGWI
                     TRRFQCFPLAPLPESHSHRVPDILYPLKTEKKECKDNQQPHCLHS"
     gene            10033..11238
                     /gene="traW"
                     /locus_tag="ACHKTW_000955"
     CDS             10033..11238
                     /gene="traW"
                     /locus_tag="ACHKTW_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraW"
                     /protein_id="HFO6462023.1"
                     /translation="MQRQPATTLLALLIGCTVIPSTHAYPVTVVASQPVTTQIMPELA
                     AIQGVMAEMVRMQTATGTAINQSSEKLASVIAQDGQATRQQMIFSNETRRLEEARKSF
                     SVPDSICSESASGVAAESRRAAASAAARLSQGGGVSSKPIRERLSRAADSPTREAYDS
                     AGIHAGYCTEAEYARFGGTDVCPAVGELPGGDSQVRSLYQGAGTADTPAALTWDQKQI
                     DAATAYMKNTARPSAGRAPGKGEVGTQTGRTYVGLQNEYNGIIDAASHPQLSLIADST
                     PNEATRGALTEALQSPSAAAYFDRTASSEARTRGHMSQREFEAFEAGRRYANTDWQQD
                     LQGMEGDNLLRELLRTTALLNWQMNDLKEQIRQGNVIAGQQLALAARQYYGQRLGELS
                     QAMSQGSVR"
     gene            11235..11804
                     /gene="traX"
                     /locus_tag="ACHKTW_000956"
     CDS             11235..11804
                     /gene="traX"
                     /locus_tag="ACHKTW_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000650756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraX"
                     /protein_id="HFO6462024.1"
                     /translation="MKDDNSTDKKKPAPRLRRIGDIGINLFAPLAETRWMLRSCRHML
                     GQHKTRLKRLMPDRQPESPVCMTWEEAVRASGLTVNELDRRLRRRRMFWRTGFWLMMI
                     PVLLLCGMALAATALPAITLIRILACLLLMSAIAALCASKTLIVSYRLWQLNERRVSE
                     DEQGTFPDYLQARHGWKQATWLAITHRQY"
     gene            11877..14042
                     /locus_tag="ACHKTW_000957"
     CDS             11877..14042
                     /locus_tag="ACHKTW_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotA/TraY family protein"
                     /protein_id="HFO6462025.1"
                     /translation="MKIIPRALSAGLALAAVPAQAFVTYQDIVSAATNPDDLSRQALV
                     TIFGDVVTNPLTASSNTIIGNLFMVFNAIIAIMGIVWFLFIGIRHVVRTGHQGNVFNN
                     GRDVVGMLSIVSGFMMIVPTASGWSLAQLVMLWGASIMGIGSANIMVQTAAENIAEGY
                     SMTVQPVHVSTRTAARGILEMALCKHAMNAGLDDFNRTARSSTPPMSESSRTENGRYT
                     VTVSNGSATCGSVSLTVKGNGSNNQSALDYFFNPFIETEYQGVLTAQRSAMDTMLRDL
                     DSAAQEFVSAYRTRRDTGNGNLPDIESRIQKAANRYEETVQKALPTDNAETERREQLK
                     SYLTTYGWVALGAWYQTFATANQRLAELADRAPTVSGVSSQGEAGDTEFLKEVKGAYQ
                     AQLQNSPYTPALGTATSASEYQASNTQDPQSAILKLIGFNLTRMTNWTATYLSGTGTT
                     TNQLNPLIKMKNIGDYTMVTTETIWAGYTTVRVLSTAGSNSIFGRLVNAVSGLSDAAK
                     AFLEAAAPPLYFLLFLLFCAGFSLSIYLPFIPFIFWMTGIGNWIVSVLIGCAAGPLWA
                     ATHLGTSQDRGSRAAYGYIYLIDSMIRPLLMVFGFFFASVAIVAGGTILNALYAAALI
                     NVQINSVTGVFSMAGFLMIYARACTTLVAGVFALQAYLPDHVINFLGGRDGVNMLGNM
                     VSSVKDIIAGGNTNIRRTPGVREEKLKNINGDDKDGIKQ"
     gene            14113..14766
                     /gene="excA"
                     /locus_tag="ACHKTW_000958"
     CDS             14113..14766
                     /gene="excA"
                     /locus_tag="ACHKTW_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid IncI1-type surface exclusion protein
                     ExcA"
                     /protein_id="HFO6462026.1"
                     /translation="MNTTKNRYPSWWGYYYLIRNTYFIFGIPFLLLCSLGFTGMMLTE
                     PYIGTDIQAYLYTCGSWFLLIIPSLWLYLRTKAKKKKILQVVKKIKESSPFAPTSDYE
                     QLSFSKSCYFGIDIKNGTMLYVRIYPNNVMDVIGLDIHNFTRTVAEDGKLEIHTTYVS
                     LPMIPLEISGISSRTLANTMHTMAARGYEYKERFPQMIRYRVKEWEKVAGVPVAEVF"
     gene            15040..16626
                     /locus_tag="ACHKTW_000959"
     CDS             15040..16626
                     /locus_tag="ACHKTW_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001506542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TnsA endonuclease N-terminal domain-containing
                     protein"
                     /protein_id="HFO6462027.1"
                     /translation="MSESRSNFPARHGTPWNTEELQRLLVSIAEGVSLKKLTQTHQRS
                     SGGIRGAIKRLLPSEFLEFNDINTVNDLSNYFNKIPRQEHNSIINNLISLSTAKTIAH
                     DKVEIQKNSFKQNTPLPLCQPNTDFKDYIADEHDVIMLIHSAIESLSNKRHQYILLMR
                     LGTDDSPHSLADIAVKLGISRERVRQLQERALGLLAAKSRYEGTPGACLKALINSIGN
                     SPYDIAVWIYRIVHRDFVTSSELAAKFIIFTAGIPKARRDEIKNSLSLISQLQKQRLR
                     ERRAELAHARAQERARIKKERVEFIFSKWLDNTHWPKYIAPPPPVEQLCSQRAVNDTD
                     ISGFFYSQKLGRTVQYESGLEFKIMNLLECCDQVLFYQEQPFSIPYCFKNKSKKYYPD
                     LLIATVDGRCLLMEVKPTDRMTLSLTRIKSNAGRKWAHTRGWGWLVISDRFSLRQLEE
                     HAISTNTWKLIEAELKTSRILAWREMMELRTRYEIHRLDLIAYIIQSGAEVDNFYRIT
                     PKISNNTSNQRQPNESDCHN"
     gene            complement(16764..16916)
                     /locus_tag="ACHKTW_000960"
     CDS             complement(16764..16916)
                     /locus_tag="ACHKTW_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001618166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO6462028.1"
                     /translation="MPQRTFLTMLIVVCVTILCFVWMLRDSLCGFRVEQGNTVLVATL
                     AYEVKR"
     gene            17248..17490
                     /locus_tag="ACHKTW_000961"
     CDS             17248..17490
                     /locus_tag="ACHKTW_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001317518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6462029.1"
                     /translation="MSNEYQSTSINPDVFEQFFTLLNTHCKSSEAALAMLSTLCYELM
                     RRSGIHSLDSLMLNGEGILIQLQGADIREKTGQHIH"
     gene            complement(17583..19118)
                     /locus_tag="ACHKTW_000962"
     CDS             complement(17583..19118)
                     /locus_tag="ACHKTW_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016242774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFO6462030.1"
                     /translation="MSCIKEKSKYAFERAKQFYFMLWCKESLIYLLFSVLVTWCCGYT
                     VKNTLGNVLILFLMLLLGRQNVISATCVCIFLLLSACYAPVGMIYGKINNSFIASVMQ
                     TNINEAGEFITGIPVLYFIVSLVIAAFTFWFWRKRDKSHRGFIFLLFFIVLSVNAWPK
                     RMVTNVMTGIAETREEMMRYESLKNNQKDSWDIVSVEKKYKTIIIVIGESVRRDYLSV
                     YGYPLPTTPWLNSAPGIFINGYFSAAPNTIGSLSRTLTLDYTETGNPGNNIVTLARKA
                     GYETWWISNQGSLGQHDTLISVIAANADKKHFLKEGSSSSLRIDDEDMLQYVREAVNS
                     SSPKVIFIHMLGSHTNPCDRLFNDAQPFRELFGNKVSCYLSTLLKLDRFLQAIYNMTT
                     EHGDDFAMLYFSDHGQSVSSDSTPVHHAPGLRSGYDVPLMMMASDITEHHLNHVHISA
                     RRFPEIFQWITGIQTKNLPLSFSLLQNDFAESVSVFNGERDVPVMSLPEEPILTGNAE
                     SEK"
     gene            complement(19318..19773)
                     /locus_tag="ACHKTW_000963"
     CDS             complement(19318..19773)
                     /locus_tag="ACHKTW_000963"
                     /inference="COORDINATES: protein motif:HMM:NF022214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 family protein"
                     /protein_id="HFO6462031.1"
                     /translation="MRDKSEVLTGAQKNRFGLILYIILLSVVYILITRLNLTTTYYSL
                     YSSLGENSQVTEYFIISIATFLFLSGLCLLFIYSFFKRKRVVGKLLLAIHLLYISHYA
                     ITFVYCLYVVGAECMTTGKIEYILIDIIIAMLSLSYVCLSGRARHIFVN"
     gene            complement(19861..20484)
                     /locus_tag="ACHKTW_000964"
     CDS             complement(19861..20484)
                     /locus_tag="ACHKTW_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001579788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO6462032.1"
                     /translation="MQERIDYHIEKYQFRARNESPRLMRQWADVMRECRETGAGARER
                     LRTALLNVDYVTSFELPFRLLLTRTPQMIDTLRREWLLSQKKALFNGRQRGCVYSLQN
                     DLSGVPDCFSYSLATRIRRMDCYGMTTRCFTDIAGQVKVPTARLKLALESGLLVTALD
                     GLFWLGTQRIAADVQRLRQSGMVIVTREVEVSDSLTGTTRLVSAYTL"
     gene            20643..21146
                     /locus_tag="ACHKTW_000965"
     CDS             20643..21146
                     /locus_tag="ACHKTW_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016242778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HFO6462033.1"
                     /translation="MIKYIFALTLLLSGSVCAVQIQGLVIRVLDGDTLEILQARHPVR
                     IRLVNIDAPEKKQPFGRWSMNQLKSLVANTPVTVTYTQTDRYGRILGRVYTEDGMEVN
                     RWLVQHGAAWVYPDFNTDYTLPAYQREARTMKRGLWADKKPVPPWLWRKENKNDHTEL
                     QINNILP"
     gene            21439..24420
                     /locus_tag="ACHKTW_000966"
     CDS             21439..24420
                     /locus_tag="ACHKTW_000966"
                     /inference="COORDINATES: protein motif:HMM:NF014452.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S6 family peptidase"
                     /protein_id="HFO6462034.1"
                     /translation="MYHITHKVDIITKLSIKQQISRYNMNKIYSLKYSSLTGGLIAVS
                     ELSKKVKGKTGRKLMTASVALSVSLSALPAEASTVSAEIPYQTFRDFAENKGVFTPGA
                     TGIEIKDKNGNAVGTLDVPMIDFSSVSRRGSLTLLSQGYGVSAKHGGLGDVNNASFGY
                     DKNNYTVVKNNKHSGLDFSLHRFSKLITEAAPADINISGQLSDSSQYTAFYRAGAGTQ
                     YIKERSGKQTHIPGTFLTGGTVGTPWYSGNNLISSSPGDTYNKSQGPLASYGQMGDSG
                     SPLFAYDSLSEKWSLAGVTLHNNGVNGQKNNWLLLPEDYIKNIITADFDPIISFNKNS
                     KEHMSWTYDAAKGVGRIQQDDQQFVMHGNLNGNLNAGKNLYFTGENGIIDLKDNVNQG
                     AGYLQFADDYTVTTSNDSSWSGGGIIVNYGTTVKWGINGVSGDDLHKVGDGTLIINGT
                     GKNEGGLKIGAGTVILEQKAKNNDSTAFSSINISGGNSRVKLSGDNQIIPDNVSWGFR
                     GGYLDINGKNTEFSRLQAVDYGAAIINSSTDKSLLTLNLSPLKKDEIAVSVKALDMNA
                     IFQGGHGTAGDLYKTTFYGPTQYYLLKKPKFGSVLMGSLKNTSEWQFAGTDLNQAVDM
                     AKNNKLTSSAQASYLYHGKLLGNMDIVIPELTGNDILTLDGSVSISGDMSKQDGALIF
                     QGHPVIHAGQTVSASQSDWENREFSLNNLNLNNADFSLSRNAFMNGNIRAVNQSTVII
                     GGDTVFTDKNDGTGNDVISVEGKSAAAGTSSYTGHITLEQKSALDIRDNFRGGVTSED
                     SHINVSSSSVLFSDASSFINSSLNIHKGGALTAQGGLFTSGSIDIGDASLLLTGTPVN
                     SDDAAFLPTINMADGGFNLMSDSSVLKARDQASVVGDIISDKQATISFGTESGKEGIL
                     SEKASRGLAVGLLSGFNTAYRGAIHAPSASATVNNTWWQLTGDSSLRSLKNTGSMTYF
                     TGSAANKALDLPLYFQTPVIT"
     gene            complement(24391..25619)
                     /locus_tag="ACHKTW_000967"
     CDS             complement(join(24391..25304,25304..25619))
                     /locus_tag="ACHKTW_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558215.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS2 family transposase"
                     /protein_id="HFO6462035.1"
                     /translation="MIDVLGPEKRRRRTTQEKIAIVQQSFEPGMTVSLVARQHGVAAS
                     QLFLWRKQYQEGSLTAVAAGEQVVPASELAAAMKQIKELQRLLGKKTMENELLKEAVE
                     YGRGKKVDSARALIARGWGVSLVSRCLRVSRAQLHVILRRTDDWMDGRRSRHTDDTDV
                     LLRIHHVIGELPTYGYRRVWALLRRQAELDGMPAINAKRVYRIMRQNALLLERKPAVP
                     PSKRAHTGRVAVKESNQRWCSDGFEFRCDNGEKLRVTFALDCCDREALHWAVTTGGFN
                     SETVQDVMLGAVERRFGNELPASPVEWLTDNGSCYRANETRQFARMLGLEPKSTAVRS
                     PESNGIAESFVKTIKRDYISVMPKPDGLTAAKNLAEAFEHYNEWHPHSALGYRSPREY
                     LRQRACNGLSDNRCLEI"
     gene            25624..26938
                     /locus_tag="ACHKTW_000968"
                     /pseudo
     CDS             25624..26938
                     /locus_tag="ACHKTW_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052685.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(27101..27310)
                     /locus_tag="ACHKTW_000969"
     CDS             complement(27101..27310)
                     /locus_tag="ACHKTW_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6462036.1"
                     /translation="MKTNAPRLFFVDKESGVSFDVFHVLSVSFPVTAVPHMTGECNLR
                     YLTENVPFAGTGGQSCMQDLYHSSG"
     gene            27444..27806
                     /locus_tag="ACHKTW_000970"
     CDS             27444..27806
                     /locus_tag="ACHKTW_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6462037.1"
                     /translation="MNDGRQTPPPPEAWKIRFLANGTPGTWFYTDDAAVTKEWLRAHC
                     DDVEVIPLCRMHDGPVFREHHINELSAALARMLHMHHILLERTRYRENIHDAECIREI
                     HEAPLQAIGILEKYKRTK"
     gene            27819..28010
                     /locus_tag="ACHKTW_000971"
     CDS             27819..28010
                     /locus_tag="ACHKTW_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000264906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6462038.1"
                     /translation="MWILIFWLSSPFNVSSGDARQPTLAVHSQAFRTEKACRDAFTAL
                     KKENVGEFALRGVCVPAGE"
     gene            28038..28349
                     /locus_tag="ACHKTW_000972"
     CDS             28038..28349
                     /locus_tag="ACHKTW_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlgM"
                     /protein_id="HFO6462039.1"
                     /translation="MTSPDLIQADLHRMSWSQLAKAAEESTVHHDYARALILWRHAYH
                     AATLTINKNLATAKINFCAKRILMRNQMSKIIRHTDTDERLFRLSKHHHLYEKKKTKE
                     G"
     gene            complement(28492..28587)
                     /gene="dqlB"
                     /locus_tag="ACHKTW_000973"
     CDS             complement(28492..28587)
                     /gene="dqlB"
                     /locus_tag="ACHKTW_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinQ-like type I toxin DqlB"
                     /protein_id="HFO6462040.1"
                     /translation="MINLLIIVLRAVVAVVNAVIALLELIRELID"
     gene            29086..30366
                     /gene="trbA"
                     /locus_tag="ACHKTW_000974"
     CDS             29086..30366
                     /gene="trbA"
                     /locus_tag="ACHKTW_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000121930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TrbA"
                     /protein_id="HFO6462041.1"
                     /translation="MSYQQTSAAEDPMAIWYIVGAICLLFAIIIWRFLPEIVFASCLI
                     LHTLWGVIDWGPFHNFAAPRYNLLAITANNAATITFSQWLDVMSRTVGILWLILLPMT
                     FGFLWMWFHHPAQPRFTRRPLNIHTLPHIFSALSPAIAPVLADGDNNRLFHGQKRPER
                     RVALTPEAFVEQNNLIRNMQLDVASTRQCFMAQLGQPLTSWKDMAPHEKALFAIFGLQ
                     FFLGDRKAAVALMNNLNLSCRLKSKRDQGRFSTPVYSLARNAFIRVIKTEGAQKWLRQ
                     HRYVRSGLVWLYAHDLRLTPPNWLWLKGVDRTLFYALHRANTTKGFIEGAGVVAVARA
                     ENEACRLGLPCPEPCVEEAIEGLRQDMLRLGLIWDEPQPDRDRRRQIRTRWSLTDDVI
                     PRRHDNDEGSDTGETTETTETTETRHPADKEKAQ"
     gene            30363..31481
                     /locus_tag="ACHKTW_000975"
     CDS             30363..31481
                     /locus_tag="ACHKTW_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HFO6462042.1"
                     /translation="MTIRYTATLNNKHLSLTALYPDGGQEVIAILPVTELGALINRNN
                     RLMHLSFSTPFREITETLLVDTVDTEPPGHHIGRMLSAIDSCLSAAYAELPARKTSFM
                     RGLIYGAGLVLALLTAVTAGYILLKPDSDSPLRVPVTESVTETAPLHIDKEIVRSVAP
                     SETKNEIVHPAPQEMQETSATPAITADKNTLVAEARRNLATVLKRNADRGMFTINLST
                     GHERTLYAFLDPACINCRLLEPALKQLAREFSVVIYPVSVIGGEASTNRTAPLLCEPD
                     AQKRATGWHQLYSADGGMAPTGDAPAAPDEKCLTAARAAIDVNNQAFRQFGFGGTPWV
                     LSDTGWHLPSGILKDATTVSLFLKATDTEDNHNEPASR"
     gene            31462..33765
                     /gene="trbC"
                     /locus_tag="ACHKTW_000976"
     CDS             31462..33765
                     /gene="trbC"
                     /locus_tag="ACHKTW_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F-type conjugative transfer protein TrbC"
                     /protein_id="HFO6462043.1"
                     /translation="MSQHHVNPDLIHRTAWGNPLWNALHNLNIIGLCLAGSIITALIW
                     PLALPVCLLFTLVTSVIFTLQRWRCPLRMPMTLSLDDPSQDRKVRRSLFSFWPTLFQY
                     EADETSPARGIFYVGYRRINDIGRELWLSMDDLTRHVMFFATTGGGKTETTFAWLLNP
                     LSWGRGFTFVDGKAQNDTTRTIWYLSRRFGREDDIEVINFMNGGKSRSEIIQSGEKSR
                     PQSNTWNPFAFSTEAFTAETMQSMLPQNVQGGEWQSRAIAMNKALVFGTKFWCVRERK
                     TMSLQMLREHMTLEGMAKLYCRGLDDQWPEEAIAPLRNYLQDVPGFDMSLVRTPSAWT
                     EEPRKQHAYLSGQFSETFTTFAETFGDVFAADAGDIDIRDSIHSDRILIVMIPALDTS
                     AHTTSALGRMFVTQQSMILARDLGYRLEGTDAQTLEVKKYKGSFPYICILDEVGAYYT
                     DRIAVEATQVRSLEFSLIMMAQDQERIEGQTSATNTATLMQNAGTKFAGRIVSDDKTA
                     RTVKNAAGEEARARMGSLQRHDGVMGESWVDGNQITIQMESKIDVQDLIRLNAGEFFT
                     VFQGDVVPSASVYIPDSEKSCDSDPVVINRYISVEAPRLERLRRLVPRTVQRRLPTPE
                     HVSSIIGVLTAKPSRKRRKNRTEPYRIIDTFQRRLATAQTSLDLLPQYDTDIESRANE
                     LWKKAVHTINNTTREERRVCYITLNRPEEEHSGPEDIPSVKAILNTLLPLEMLLPVPD
                     LTASPPHKKNVAQTPSGGKQESRKKRF"
     gene            34244..34474
                     /locus_tag="ACHKTW_000977"
     CDS             34244..34474
                     /locus_tag="ACHKTW_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6462044.1"
                     /translation="MKTNMGSTKNGARVPHVLNRFTSMDIDMNSQILPDMLINFALIN
                     ITDRKNEGTNTIDGNWQADEGRRYRDNVRIYF"
     gene            complement(34572..35003)
                     /locus_tag="ACHKTW_000978"
     CDS             complement(34572..35003)
                     /locus_tag="ACHKTW_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0019534 - toxin transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity TolA C-terminal
                     domain-containing protein"
                     /protein_id="HFO6462045.1"
                     /translation="MVLRSIWIEEQYMGVKKIVMLAILVSFVAGCAPLHPSNCHKTTA
                     LGSCSSGRWDDQDEWGEQARGIRDAINAKLDDPQKWKRKKCRLHMEFSRDGTALKIST
                     SNGDKAYCEAIKSAAHKAKFPAFNNPEVYRDFQKSGFDMRG"
     gene            complement(35071..37782)
                     /locus_tag="ACHKTW_000979"
     CDS             complement(35071..37782)
                     /locus_tag="ACHKTW_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFO6462046.1"
                     /translation="MNAIIPHKRRDGRSSFEDLIAYTSVRDDVQENELTEDSEIKSDV
                     PHRNRFRRLVDYATRLRNEKFVSLIDVMKDGSQWVNFYGVTCFHNCNSLETAAEEMQY
                     KADKAVFSRNDTDPVFHYILSWPAHESPRPEQLFDCVRHTLKSLELSKHQYVAAVHTD
                     TDNLHVHVAVNRVHPETGYINWLSWSQEKLSRACRELELKHGFAPDNGCFVHAPGNRI
                     VRKTALVRERRNAWRRGKKQTFREYIAQMSIAGLREEPAQDWLSLHKRLASDGLYITM
                     QEGELVVKDGWDRAREGVALSSFGPSWTAEKLGRKLGEYQPVPTDIFSQVGTPGRYDP
                     EAINVDIRPEKVAETESLKQYACRHFAERLPEMARNGELESCLDVHRTLAKAGLWMGI
                     QHGHLVLHDGFDKQQTPVRADSVWPLMTLDYMQDLDGGWQPVPKDIFTQVIPGERFRG
                     RNLGTQAVSDYEWYRMRMGTGPQGAIKRELFSDKESLWGYTTVQCESLIEDMIAGGNF
                     SWQACHEMFARKGLMLQKQHHGLVIVDAFNHELTPVKASSIHPDLTLSRAEPQAGPFE
                     IAGADIFERVKPECRYNPELAASDEVEPGFRRDPVLRRERREARAAAREDLRARYLAW
                     KEHWRKPDLRYGERLREIHAACRRRKAYIRVQFRDPQLRKLHYHIAEVQRMQALIRLK
                     ESVKEERLSLIAEGKWYPLSYRQWVEQQAVQGDRAALSQLRGWDYRDRRKDKRRTTNA
                     DRCVILCEPGGTPLYEDTGVLEARLQKDGSVRFRDRRNGELVCVDYGDRVVFYHHQDR
                     NELVDKLNLIAPVLFDREPGMGFEPEGSYQQFNDVFAEMVAWHNAAGITGNGHFVISR
                     PDVDLHRQRSEQYYHEYIRQQKSISGGHGASYAPVQDNEWTPPSPGM"
     gene            complement(37794..38126)
                     /gene="mobA"
                     /locus_tag="ACHKTW_000980"
     CDS             complement(37794..38126)
                     /gene="mobA"
                     /locus_tag="ACHKTW_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization protein MobA"
                     /protein_id="HFO6462047.1"
                     /translation="MSEKKTRTGSENRKRIVKFTARFTEDEAEIVREKAESSGQTVST
                     FIRSSSLDKVVNCRTDDRMIDEIMRLGRLQKHLFVEGKRTGDKEYAEVLVAITEYVNA
                     LRRDLMGR"
     gene            38359..38694
                     /locus_tag="ACHKTW_000981"
     CDS             38359..38694
                     /locus_tag="ACHKTW_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000157082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobC"
                     /protein_id="HFO6462048.1"
                     /translation="MASKKFYSDDDIQLAKAALSELPDLTAQRKTLRDFLDAIRDDII
                     VLVRTKGYTLADVRDTLKNAGYEVGEKALRDIIREAESKKPSRRSSSKTAPKKTDSGR
                     KDGIDMNNN"
     gene            complement(38780..39628)
                     /locus_tag="ACHKTW_000982"
     CDS             complement(38780..39628)
                     /locus_tag="ACHKTW_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFO6462049.1"
                     /translation="MNQTLPTADLNTAGTTDVIPSVAIDRIIAQRNEGIALFMQAMEC
                     LATARKILLDASGDIFLYGFEDCVTDSVRRMDKQEEAKRNITRLADRKIWDRLMTDTG
                     MYTFMSSCQRDEWNSQLMSDTCPEITLDNVLATFRHLNARKMQTFEQGLIDVYRKLSW
                     DYRTNNPCRLGKKIIIENLLYRWSNGRVTLDCSGREALDDLVRPFYLLEGRNVPDFRN
                     SIGAQYGEFLGNGDNVGKLLEGEYFTVRGYQKGTVHIVFKRSDLVEKLNDIIARHYPD
                     ALPPRV"
     gene            40209..40460
                     /locus_tag="ACHKTW_000983"
     CDS             40209..40460
                     /locus_tag="ACHKTW_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003980153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFO6462050.1"
                     /translation="MTGWELRIWRKSMLWSREKAAREFGVTQRTWHAWENAEQVDVTV
                     WRTTQALSVRDLLPHMQGMRKADIIRRLENELGETAGNV"
     gene            complement(40491..40736)
                     /locus_tag="ACHKTW_000984"
     CDS             complement(40491..40736)
                     /locus_tag="ACHKTW_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014358130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6462051.1"
                     /translation="MRTTKPGKMPGFFVSRSRANSMTIAERLIQKGFDEGFKQGALEV
                     AREIACRLRDIGWPPERIQEVTGLSGEELKKLFPDEQ"
     gene            complement(40736..41674)
                     /locus_tag="ACHKTW_000985"
     CDS             complement(40736..41674)
                     /locus_tag="ACHKTW_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HFO6462052.1"
                     /translation="MSKKKNTTTPTPHDAAFRSFLANPDVARDFLELHLPAEYRQLCD
                     LSTLKLEPATFVEPDLHQYASDILWSVKTTGGEDGYVYTLIEHQSTENLYMPFRMLRY
                     SVAAMQRHLEQHKTLPLVIPVLFYHGERSPYPYSMNWLDCFENPALAAKIYTKPFPLV
                     DITVVDDNEIMNHRRMAALTLLMKHIRHRDMMELLDKLPQVMVEISDEQVRVLIHYIV
                     NAGDSVSPEFMRALAERLPQHEDKLMTIAERLEQKGRQEGRMEGRMEGRMEGRMEGAL
                     EKALAIACQLQKMGMTPEQIKQATGLSDDELKKITH"
     gene            complement(41671..42060)
                     /locus_tag="ACHKTW_000986"
     CDS             complement(41671..42060)
                     /locus_tag="ACHKTW_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein CbiX"
                     /protein_id="HFO6462053.1"
                     /translation="MKSCFDTDKDFQMSSDEVTFNADSVRSKLELLSEELMHRCGLVY
                     ELYQRRLSAAIDNFVSTLPTEQHLLVLELAREEFDYLSAEEIAEEIRRDAERGYCCHG
                     FDRNCCPLGCGDLDDDCCDAADLMEEP"
     gene            complement(42251..42517)
                     /locus_tag="ACHKTW_000987"
     CDS             complement(42251..42517)
                     /locus_tag="ACHKTW_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6462054.1"
                     /translation="MRPSIIFATAEYVKRLREECLRENKPLHRHTRFRRQELAPDEIN
                     PDVLAMGGHIARRCSERKRVRIPAMKVSEWGHLLRALEIERVCH"
     gene            complement(42610..43044)
                     /locus_tag="ACHKTW_000988"
     CDS             complement(42610..43044)
                     /locus_tag="ACHKTW_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000218847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="post-segregation killing protein PndC"
                     /protein_id="HFO6462055.1"
                     /translation="MTVSSTISVFCRDGVFRTVYCHLHGEPTWNGRILHTHYATGQLA
                     EALVEHGDIRCLGPRCDKPAGHTLQNPVDGVTAYYGRDSGFRMDSEAREYRSFREAIA
                     TESTEEVRFHYVFIDGYWKVMYRTPEGWKMKALALALRRCPE"
     gene            complement(43775..44275)
                     /locus_tag="ACHKTW_000989"
     CDS             complement(43775..44275)
                     /locus_tag="ACHKTW_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFO6462056.1"
                     /translation="MSVVAPAVYVGTWHKYNCGSIAGRWFDLTTFDDERDFFAACRAL
                     HQDEADPELMFQDYEGFPGNMASECHINWAWVEGFRQARDEGCEEAYRLWVEDTGETD
                     FDTFRDAWWGEADSEEAFAVEFASDTGLLADVPETVALYFDYEAYARDLFLDSFTFID
                     GHVFRR"
     gene            complement(44738..45334)
                     /locus_tag="ACHKTW_000990"
     CDS             complement(44738..45334)
                     /locus_tag="ACHKTW_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000978013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6462057.1"
                     /translation="MMKSDEQYQVPVWMRPLLPLLCNTGGNDPEELLNDTETTASANI
                     VRYVLIVAVRSQIDLLQLLYRKGLLRTEIPGGFSPEEAQELLDNLVRSHISKALSGER
                     MAARDRNADLTWIRQQLVDAAWFVRATLEAHGMGVGNESPSAPPETMPDIQTRELVML
                     IKRLASSLKAVKPDSSVVREAQDWLCDRKLVDITDILR"
     gene            complement(45331..46050)
                     /locus_tag="ACHKTW_000991"
     CDS             complement(45331..46050)
                     /locus_tag="ACHKTW_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001723065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
                     /protein_id="HFO6462058.1"
                     /translation="MSARSRALIPLSAEQQAAMQAVAVTEQRRRQGRTLSAWPYASAF
                     FRCLNGSRRISLTDLRFFAPALTKEEFHGNRLLWLAAVDKLIESFGEVCVLPLPSDAG
                     HRLFPSVPFREGERRRQKTTLTEQKYSRQREREAERRELEYQTCFAQAQIDLAFHTPS
                     TVGSWLSRWSGVVEEHDLETIFWGWCGRFPSLSSFDRFFWQEEPLWRLIFEAGEAGRG
                     APVQVRALEQWMIPNKLENAI"
     gene            complement(46047..46481)
                     /gene="psiB"
                     /locus_tag="ACHKTW_000992"
     CDS             complement(46047..46481)
                     /gene="psiB"
                     /locus_tag="ACHKTW_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000845953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugation system SOS inhibitor PsiB"
                     /protein_id="HFO6462059.1"
                     /translation="MKTELTLNALQSMNAQEYEDIRAAGSDMRRNLTHEVMREVDAPA
                     NWMMNGEYGSEFGGFFPVQVRFTPAHERFHLALCSPGDVSLLWMLVLVNCGGQPFAVV
                     QVQHIFTPVAISHTLALAATLDAQGYSVNDIIHILMAEGGQA"
     gene            complement(46536..48494)
                     /locus_tag="ACHKTW_000993"
     CDS             complement(46536..48494)
                     /locus_tag="ACHKTW_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFO6462060.1"
                     /translation="MPVTKCEPETTRKASRKSVKTQETVLSALLAQTEEVSVPLASLI
                     KSPLNVRTVPYSAESVSELAESIKGVGLLQNLVVHTLPGDRYGVAAGGRRLAALNMLA
                     ERGIIPADWPVRVKVIPQELATAASMTENGHRRDMHPAEQIAGFRAMAQEGKTPAQIG
                     DLLGYSPRHVQRMLKLADLAPVILDALAEDRITTEHCQALALENDTARQVQVFEAACQ
                     SGWGGKPDVQTIRRLVTESEVAVAGNTKFRFVGADAFSPDELRTDLFSDDEGGYVDCV
                     ALDAALLEKLQAVAEHLREAEGWEWCAGRMEPVGECREDAGTYRSLPEPEAVLTEAEE
                     ERLNELMTRYDALENQCEESDLLEAEMKLMRCMAKVRAWTPEMRAGSGVVVSWRYGNV
                     CVQRGVQLRSEDDVADDADRTEQMQEKASVEEISLPLLTKMSSERTLAVQAALMQQPD
                     KSLALLAWTLCLNVFGSGAYSKPAQISLECEHYSLTSDAPSGKEGAAFMALMAEKARL
                     AALLPEGWSRDMTTFLSLSQEVLLSLLSFCTACSLNGVQTRECGHTSRSPLDSLESAI
                     GFHMRDWWQPTKANFFGHLKKPQIIAALNEAGLSGAARDAEKMKKGDAAEHAEHHMKD
                     NRWVPGWMCAPHPQTDATERTDNLADAA"
     gene            complement(48553..48786)
                     /locus_tag="ACHKTW_000994"
     CDS             complement(48553..48786)
                     /locus_tag="ACHKTW_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 domain-containing protein"
                     /protein_id="HFO6462061.1"
                     /translation="MSEYFRILQGLPDGSFTREQAEAVAAQYRNVFIEDDQGTHFRLV
                     VRQDGTLIWRSWNFEDGAGYWMNQYIRDFGILK"
     gene            complement(48844..49371)
                     /locus_tag="ACHKTW_000995"
     CDS             complement(48844..49371)
                     /locus_tag="ACHKTW_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFO6462062.1"
                     /translation="MSARGINKVILVGRLGNDPEVRYIPNGGAVANLQVATSESWRDK
                     QTGEMREQTEWHRVVLFGKLAEVAGEYLRKGAQVYIEGQLRTRSWEDNGITRYVTEIL
                     VKTTGTVQMLGRAPQQNAQAQPKPQQNGQPQSADATKKGGAKTKGRGRKAAQPEPQPQ
                     PPEGEDYGFSDDIPF"
     gene            49573..>49800
                     /locus_tag="ACHKTW_000996"
     CDS             49573..>49800
                     /locus_tag="ACHKTW_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6462063.1"
                     /translation="MAKNFVAGASHPGQMEREGVTSAPRKVHSGKQAAGVAAGGKTAA
                     PSGAVSSHTVRCAGSAGHGATGAARPERSLQA"
BASE COUNT        12627 a        13572 c        12073 g        11528 t
ORIGIN      
        1 tcacaggccc cctgccgcca cctgcctctc cccttgttca ggaagccgca tcactggatt
       61 cccccctgaa tgcaggtgaa attcgggaac tgcggggacg aatggccgat aacgaacggg
      121 ccatcagcgc acctgtcacc agtgtggtgc cccgtatcag ctccctcaca gtaaatctgt
      181 cacccggggc cagtctgccg cttgtccgaa ccgcactgca taacgtcagc gtggtaacgt
      241 tcacggacat caacggctcc ccgtggcctc agtcggatcc gccttataac gcggcacccg
      301 gtcactttga tgtgcagttc aacggcaata tggtctccgt cactcccctg cgcgcctggg
      361 ctgcagggaa tgtaacggtc tacctcaaag gactgtctgt accggtcatt ctgaatatca
      421 ccagtggtga aaccgacacc aataccctga gtcaggagat cgacagccgt ctggatctgc
      481 gcattccccg tcagggacct gtcagtcctg tggtagccgt accatcagag aagattgccc
      541 tgcatgatgc caccctgcag gcctttctgg acggtgttcc tcccgatgat cccaccgtgc
      601 gtcggctgaa attctccgga aatgttccgg acaccaccat ctggcagcac ggcgatgatc
      661 tgctcgtgcg atcgcgggcc atgctgcggg atgaatttga acagacgctg gcatctgctg
      721 acggtacgcg tctgtggaaa ctgccggtga ctcccttact caccttctcc gtgaatggtc
      781 agtccattca cgtcacccct gaactggagt aaatcatgag cgcagaacag gacacagccg
      841 caaagtccgg caaaaaactg tcagccctgc tggttctcgg gggcgtgatt atcatcgggg
      901 gcggatacct gctgctcacc tggctttccc ccgaaaacag cgcatcgctc tcgtcagtca
      961 atatcaacag cacgacaaca ggcagcgggc ggaccgtggt ggagtcacag cactaccggg
     1021 atttactgcg tgctgacaat gagcgcgggg ccgcagaagc attacgtaat aaccagacgt
     1081 tcatcgccag tctgcccagg ggactggata ctgatcacgg aaagaatcaa agtagggata
     1141 ctcagcctgc tgaccaggcc gagggttctg gtcaaccatc aacgccccct caggcagatc
     1201 aaacagcccg ggaaaaacag cgggaagcgt tacaaaaact gcttgcccgc atcaacacac
     1261 agcatcctga cgcccgcccc ccggtagtgg cggctgcaat gtggggtacc actggtgctc
     1321 ccgggataag ctcacacgcc tcaccacccg gtaccggcac atcaccacaa ccacgtctgc
     1381 aggaggccag tctggcaacc ccggcagctc gtacagggat acagctgata ccggcactga
     1441 cccgcgtacc tgccttcatt gatacggaag tggactctga caatgctgtc tcccgcgtgg
     1501 tggcctccgt acctgccggc ccatgggccg gggcagtcct ctacagccag gacgccagac
     1561 tcgccggcaa gggaatggat atccactttg accggatgat gtggcggggc atgtttctga
     1621 aagtcaatgc atatgccctg aatgaacata ccggcatgtc atccgtggcc agcgacgtga
     1681 accaccgctg gtttaagcat atcgttctgc cgtctgtgct cggtggggtg ggcagtgtcg
     1741 gaagtcttta caaagacgcc aacacacagg tcattcaggg aaactacggc agcattaccg
     1801 gcagggtggg aatgcctgat ggcgaggcgc tggctggcgt cattgcaggc ggcatggccg
     1861 aacggggcag tcagttgctg acccgccagg cggagagcga accttaccgg caggtgacag
     1921 tgtcacgcgg agaggttgtc tccattctgt ttgttgagcc ggtcatgagc aacgatatca
     1981 gtcccccctc ctccggcact gccccttctg tgccaccgcc ctccggacgc ccgctcaccc
     2041 aggaagaagc agagcgtcag gcgtctgaca ggatccaggc agctgtcatg cgtaaacaga
     2101 acgaaatgcg gcaacgttac ggaatacaac aggaaagtca tccatgaaaa acgaacatga
     2161 agatcccggc agttttggcg aaacaacgac accggcatcc ggcaaatcct tcctgaaacg
     2221 ccagtatatg ggatacagcc tgccagtctg ggccggctgt gtcctcatca tcctggccgc
     2281 agtctggtac aagtttctgc ggacaccctc agagccccct gcagatgcag gcttcgcggt
     2341 cacaccgccc gcagccgaaa gctgggcaga gcccatgccc atgacggaga ccgggagcgg
     2401 aaacactatg tccgcacctc ctgtcgcccc acaggcagac atgcagcagc ttgcccgtga
     2461 cctgaaagca gagctggaca gacgggatga ggaaatccga cgtaccctga ccatgctgaa
     2521 agagagcatc acaacccttg gcgacaccat acggaaagat gaggcctggg cacaggaaac
     2581 ccgtcagttg cttgatgaaa cccgtaagca ggtgcatgcc ctgcagatgc agggaccact
     2641 gcaaatcccg accgggaata acggccagtc aacgaaaacc ccgccttccc gcctgcaggg
     2701 catgaaaatc atgtcggtgg aaaaaggaat ggcctggatc cgctggcagg gtagcgcctg
     2761 ggcagtacgg gaaggtgaca ggctggggaa tatcaccatc acccggattg aaccgtccag
     2821 ccgctcagtc atcacctctg ccggtacatt acgatgatga gcattgatgc cctgacagcc
     2881 atagagaact ttgccagcgg tatattcagt gcaggcatgg agtttctctt cacctgggga
     2941 gagctgctgg ggattatcgg cctgattggt catctgatgc gtgcccgggc tgaagggcgt
     3001 cacagtatgg gaccgggaaa atttatcgcc ggtatcgtca tctgcggcat gctggtcgcc
     3061 ctgccatcct tcatcaatgc cgggggtacc cagatgggat tcagggcaga ttccttcggc
     3121 cctatcgctt atgtacaacc aaccaccttt ggtgccgctg ccggtgccgc caatgccatg
     3181 ctctcactgg taaaactggc cggagtgggg tttgccatga acggcatttc catctggcgt
     3241 aaatccctgc tggacgggca taccgccctg agcagcagtg agtccatcag caaaggaaac
     3301 gtcaagtttg ttgccggcgt tctgctggtt tttatcgatc gcgtcatcga tgcctctctg
     3361 gcgagtcttg gtctcgcctt ctgatccaca tcctttttca gtaattcagt ctgttagtta
     3421 aggagactat atgtcacgat tccgtgccat ggcggcgcgt ctgtgcgctg catttattta
     3481 tgttcagtta tgcctggtgg cctgccgtga tcggctgata cgcctgttac ttctgcttcc
     3541 cgtgatactg ctgccccaca cagcgatggc aaacacccta ttcgatatga tcaaaaccgt
     3601 tggcgatggt gcaaaaggct ccagcagttc atttgtggct atcggtcagg ttattggcct
     3661 ggtgctcttt atcggcggcc tgcttggcct caaacaaatc ggtaaaaaac ccggtgccgg
     3721 gctggcctgc ctgctgtcaa ttctggtcgg cctctgcctt gccgtcgtac cggaactgat
     3781 gggtagcgga cagaaaacgc tcggtctttc tccggtttca atcagttaat ccgcaaaggc
     3841 accgggaaac cggtgccgtc ttcacacaat gaggaggacc agaccatgtc ctgtgagctg
     3901 ccccctttca ccctgctcag aacagagcac ggcaacgtga ttgcccgcga caaccgggat
     3961 aatcaggagt ttgtgtttgc agaatgttat tccaccgcca tcgcagcact gattctggct
     4021 gccgtcaccc tgtatacaga gagaaaaacg tatgaacaaa acaccgccac tcatcgcccc
     4081 ggtccgggaa cgctggcttt tcccgctccc gcaaccggac acggtattca gcggtgaatc
     4141 cctgctgcca ccaccagtgc tctcaacacc cggacgctgt ccctgctgtc gcaggaccgt
     4201 cactcaccgc ttcattctgg aggattcatg gccattacag cagatggctg acacctgccg
     4261 ggatacggtc gtcctgctgg aaaaaaacct cacccgggtg atgcgcctga aaaaacatcc
     4321 ggtaccggaa aatgctgacg aaaaaaagaa acacacccga accctgcagg atgcggagcg
     4381 gtccctggca caggcccgcc tgtcggccag gcgactggcc ctgcgtcatg tggaaaagtc
     4441 acaaatcgtc acaacagatg cactcagcga aaatgaaagt gaactgctgc agccggaagg
     4501 cccgcccttt catctctgtg ccttctgcca cgcctggcac tgcctgaacg gctacgctgc
     4561 cgcacagggg gtgatggtct ggctccctga tttgcatccg gcctcggtag ttgctctcaa
     4621 tgcacgggca ctgaaggaaa ttttcagcga tgagcgtaag cgagtcagac agggacgggc
     4681 cgtactgaat gccctggtac agaaccgcct ggccgttgag gagaaatttc gcacctggcg
     4741 tccggcggat tttgctgatg cactgcgccg ctggccgccg gcacagcgta aaacgttgag
     4801 ggagaaaatg gacggggtgg cgctcattct gctgccggat tcgtttcctg acaaaaagta
     4861 cgtcatgtga cacacaccgg taccgatgac attctgccgg cacacattca acttacagag
     4921 gaacaaaaat ggatacacac tctgccggat atcagttcac tgcagattca ttccggcgtt
     4981 ttattatacg gacgctgact gacaatattc cgcctgacgg agatgaaaaa gaaattgccc
     5041 ggttcattct ccgccacccg gcatttaacg gcggctggct gctctttctg gctgcagaaa
     5101 ttcatgaaag atcccgtacc cataaaagca tcaccgtcac agtgagcgtc gataactatc
     5161 tctcatggat ggatattgcc gtatccggtc tcccggaatt accggatatg cccttctgtg
     5221 attttttcat tcgccgccag cagtttatct atccgggtgt gcccccgaca aaatcaggag
     5281 aagaatgatg aaaattcacc gcatatcccc ggagacgctt ataacactca tccacgcaca
     5341 cctggccgga aaaactgaca gtaccgccaa agaagagcac cggctactcc gtcgtttcct
     5401 gcgggatgat gacggcaggc tggccggcgt actgctgaat attgccggaa tacttcagtt
     5461 caacagggaa ttaagtgcgc ggcataatta tccggccaca cccctgactg aattttcatt
     5521 aaggaaacgg ggaaaacaac ttcatctctg tctctgcagt ctccgtttct tttatattcc
     5581 tccggtattt atacaaaaca aaagaagaaa atctattgtt gtacatctta ataagatcac
     5641 ttataaacaa acccattcaa tacgctgacg aagtgattca tactgattat actcatcttt
     5701 gcctatatgt tttttaagcg tattaacaat atcatattga ggatgtcctg aattgtcagt
     5761 atacagtgtt gttgcaggaa ggactgcata atctgatatt tcacaacctg tatacagttc
     5821 tttgtcagtt cggcaggaat ggcaaagaga aagacatgta tacaggtctg tgttttctgc
     5881 ctcctcagga gataaccacg acacatattg caaatcatct gtatcaggat aacaattttc
     5941 ttcctgaacc tgaactctga aaatatagaa aaataattca tcttttgaaa ggcatgctat
     6001 tttattcccc caatgcaccg cattatcaag ttgatccgat aaataaatcc atcctggtgt
     6061 cgtctctaac gaccagcata taacagaagg agcatcagtt cttattttcc catcttttat
     6121 tatttgcaga cctctgctca ttgttgtcgc atgaaaaaga tttaatattt tattttcagc
     6181 cttcactcta tttcctcatt tcccaagaaa catattaaaa acggaaaaca ttacacataa
     6241 cctggaggac aaatcatgac tttaaatttc cctgccgggg aaactacact attctcagca
     6301 aaaaacatcc tgacagcaat actcactctg cacaacttac acgcatagcg ttcccatttg
     6361 aatacagttg cagcccgcga tttattttgt cttccggagc cacctctgcc atgaatatca
     6421 accgcattat ttttgccatt gaagactgta tcagtaccct gtcacggtat tccgtttccg
     6481 cattcttccc cgattactgt gatctgcaca ccgtcatcgg tctggaccag caggaccgcg
     6541 aacgccgccc ctggctgaaa gccccctaca ttgccaccac aaaacagggg tattaccttt
     6601 ccgtctttga aatcagcggt gccctgaaag aaatggatga aaacccggac ctgacagccc
     6661 ccggcacact ggaatcactg attacagcgc tggcagagcg aatgaacact gcctataaaa
     6721 acaccggtca caaaatcagt tttgttttcg aacgcgatcc ggagcgtgga agggatgaaa
     6781 tcgaagccat gctcttaccc cagcgaaaat ccatcgcacg cacggggatc ctgcttcagg
     6841 acgttctgga tgaaaaagtc accacgctca ccccctggct tgtccgggaa cgctgctggc
     6901 tgaccgtctg gagctctgaa gaactgctga gccggaccga tcgcaaggac caccagaccc
     6961 gtatcaggaa actcgctgaa cacgctccgc cagcccgatt tgcgcaggat ccatggcgct
     7021 ggacactttc cgcactcaaa atacggcatg acgcctttct ggacacgctg gagcaggccc
     7081 tgacacatga ttctgatggt ctgctggttc gcctgatgga tattcatgaa gtgggacggg
     7141 aaatccgccg tcaactggag cgcaacagca cacctgcagt atggcagcct catctgcctg
     7201 aagatgcccg tcctgccggc tggcgacagg gcgaggatac ctctgtattc cacgcaccgt
     7261 cgctgaatct gcagttattc accacacagc ctgaaacaca cggcagcctg ataaaggccg
     7321 gtgacctctg gcacggtatg gtcgccatta ccctgccacc ccagaatctg cggacattca
     7381 accagctggt cagggatgtg ccccacgcaa tcccctggcg tatccggatg gatctgatgc
     7441 ccggcggcat gaaggcgctt ggggtcaaga aaaccctgct gacctacagc agctttattt
     7501 cccctctccg gcccatgtat gactcggtga tgatgctgaa tgacatcaac aaacatgatc
     7561 cggtctgtat catgactatt gttgccacca catggggaaa cagccgggaa gtctgtaccc
     7621 gcaaccaggc tctcctgaaa tctgcgctgg aaggctgggg agtatgcggc accaccacca
     7681 ccttcggtga tccgagacgt gcctgggtga acacggttct ggcggcctcc cacggttccg
     7741 gtccagtccc cctttacccg cccctttcac acgccctttc cctgtttccc ctgaacagag
     7801 ccggctctgt ctggcgggga cagggaaatc tgatgatgca caccgaagac ggctctgcat
     7861 gggaggtcgc gctggcatcg tcacaacaga acaagcacac ggaactgacg cccggcgcac
     7921 ccggtctggg caaatcggta ctcatcaacg cactgtcgga gatccagata gcctcagccc
     7981 agaagaacct gcctttcatt gcctatatcg acaagggatt cagtgcacag ggactggtac
     8041 agcttatccg tgacagcctg ccggagcaga gaaaggacga agccgtgggg atcattctga
     8101 gcaatgatcc ggaacacacc cgtaacctgt ttgatgtgat gtacggtgct aaaaaacccg
     8161 ccaccccgga aaagaacttc atggtatctg tactctgcgc actgtgcgtg gataccggca
     8221 ccggtcagcc ctgtaatccc ggtgacaccc ggcaaatcat aagcaacctg gtggatctgg
     8281 ccttcaggga gtatggcgaa aacaacccca gactgtaccg tgcaggaaca gagccccttg
     8341 tggatctcgc cctggaagag agtggcatcg cagaacaaca tgacgctgga tggtggaatg
     8401 ctgccacctg gtttgaaatc agggacatgc tgcatatggc tggcaatatc cccgcagccc
     8461 agcgggcaca ctaccaggcc atgcccctgc tggctgaaat gtccgccctg ctgggacagc
     8521 catccatccg ggatgtgttc ggcacagttc agcgcgataa ctcagcagaa ttgttactgg
     8581 actacatccg ccgtgccctc gatcagggac acagtgacta cccgatgatg tccggctgta
     8641 cccggtttat gctcagcccc gacacccgcg ttgtggccgt tgatctgaac aatgtggccg
     8701 gtgacaagac acctgccggg cgtctgcgca ccggcatcat gtacctgctg gcaggacaaa
     8761 tcgccggcgg ggattttgtc ctgccgcagt atcaggagga aatacggaaa aatctcgacc
     8821 cacgctacca tgaggtgatt ttccgccgca ttgaacagct tgatcaggag gtcaagacca
     8881 aagtctatga cgaactgcac aatgcaaaag ggatcaattt catctgggaa gccctggaca
     8941 cccaggagct ggagcagcgt aagttcggga tacgcacagt gctgagcacc cagctattgc
     9001 aggactatcc accggcagtt ctcaaatccg ccaacaccct ctggttgctg cgttaccgcc
     9061 ctgacgaaat tccgtttctg cgggataatt ttggtgtccc tgaagtcacc ctgcggcgct
     9121 tcctgaaaat gccggaaggg gctgcaccgg atggcagcgg tgtcccggta ctggctgtgt
     9181 tccgtgtaaa aaacggtacg ctggcccgga tcctgaaatt cactctcggt ccactggagc
     9241 tgtgggcact gaattcctca cccaaagaca gcgccctgag acgtgcgctg acgcaggagc
     9301 tcgggtcact gcgggcacgc cagatcctgg cagaaaactt tccccgtggt tcagccacct
     9361 cactgataga acaccgcgcc cgtacacatg acagtgaaaa cgtcatccat gatctggctg
     9421 cagaactcat ccgtaagcag ggatatcacc tctgatgtac agcatacaca tcactccccg
     9481 ctgccggcgt cctgcacggg gacaggtctt cctgaaaacg ctgcgcgtgg acggtgacag
     9541 tttcagcttc accctgccgt ccgcctgctt cacgctgcgt ccctccccca atccccgggt
     9601 cattttcgcc agtgacagac gggctgacgg ggaaatgagt ggcgttcgcc tgcctctgcc
     9661 cggagagaca ggcacgctga taacaacggc cacctggcag gtcaacgtgc cggagcggcc
     9721 tccttttgat atacagcatg tcattcactg gctcctgccc tcgcctctca cggcgctgat
     9781 gtccgacaac acctggctgt ggccggatag ccccggcaca ccggcactga cggcgggaga
     9841 ctggccggta atggaaaccg ataccccgac gctgaaacgc gtgaagcgtc gtaccttcac
     9901 gctcatcgat aaccgcaacc gtcagggatg gatcacccgt cgcttccagt gcttcccgct
     9961 ggctccgctg ccggaatcac acagtcaccg cgtaccagac atcctgtatc ccctgaaaac
    10021 ggagaaaaaa gaatgcaaag acaaccagca accacactgc ttgcactcct gataggctgt
    10081 acggttatcc cgtccaccca cgcctatcct gtgaccgtag tggccagcca gcctgtcacc
    10141 acacaaatca tgccggagct ggccgccatt cagggcgtga tggcagagat ggtccgtatg
    10201 cagacagcca ccggcacagc catcaaccag agttcagaga agctggcctc tgtcatcgca
    10261 caggatgggc aggccacccg tcagcagatg atattcagta atgaaacccg ccggcttgag
    10321 gaagcccgga agagcttcag cgtgccggac tccatctgca gtgaatccgc ctcaggagtt
    10381 gcagctgaaa gtcgccgtgc agcagcgtct gcggctgcac gtctgagtca gggaggaggc
    10441 gtcagcagta aaccaatccg tgaacgtctc tcccgggcag cagactcacc aacccgcgag
    10501 gcatatgaca gtgcaggcat tcatgccggt tactgcacgg aagctgaata tgcccgcttt
    10561 ggcggaaccg atgtctgccc tgccgtgggc gagctgccgg gcggagacag ccaggtgcga
    10621 tcgctctatc agggtgcagg cacagcagat acccctgccg ccctcacctg ggatcagaaa
    10681 cagattgatg ctgccacggc ttacatgaaa aacaccgcca gaccgtcagc gggtcgtgcc
    10741 ccggggaaag gtgaagtggg aacacagacc gggcgcacgt atgtgggcct gcagaatgaa
    10801 tacaacggca tcatcgatgc cgcttctcat ccgcaactgt cactgatagc tgacagtacc
    10861 cctaatgagg ccacccgtgg agcgctgact gaagccctgc agtcaccatc cgctgcggca
    10921 tattttgacc ggaccgcatc atcagaggcc cgcacccgtg gtcacatgag ccagcgggag
    10981 tttgaggcct ttgaagctgg cagacgctat gcaaacaccg actggcagca ggatctgcag
    11041 ggcatggaag gcgataacct tctccgtgaa ctgctgcgca ctaccgccct gctgaactgg
    11101 cagatgaacg acctgaaaga gcagatacgc cagggtaatg ttatcgccgg gcagcagctg
    11161 gcactggcgg cacgacagta ttacggacag cgtctcggtg aactgtcaca ggccatgagc
    11221 caggggagcg taagatgaaa gacgacaaca gcaccgacaa aaaaaaaccg gccccccgtc
    11281 tccgccgcat cggtgacatc gggataaacc tgttcgcacc actggcagaa acccggtgga
    11341 tgctgcgcag ctgccgccat atgctgggcc agcataaaac gcgcctgaag cgcctcatgc
    11401 ccgaccggca accggagagt ccggtctgta tgacctggga agaagccgtc agggccagcg
    11461 gccttacggt caacgaactg gatcggcgtc tgcgacgtcg caggatgttc tggcggacag
    11521 gattctggct gatgatgata ccggtacttc tgctgtgcgg catggccctt gctgccacag
    11581 cgcttccggc catcaccctg atacgtatac tggcctgcct gttactgatg agtgccattg
    11641 cagcactgtg tgcctcaaaa accctgattg tgtcataccg cctctggcaa ctgaatgaac
    11701 ggcgggtcag cgaggacgaa cagggcacct tccccgatta cctgcaggcc cggcacggct
    11761 ggaaacaggc cacatggctg gccattaccc accggcagta ctgaccgtcc ggtctgcaat
    11821 tccttttcct cattccggag gctgatgccc tgcatccctt cggttccgga gttatcgtga
    11881 aaatcatacc cagagcactg agtgcgggtc tggcgctggc tgccgtacct gcacaggcat
    11941 tcgtcaccta tcaggacatt gtgagcgccg ccaccaatcc ggatgacctg tcgcgccagg
    12001 cactggtcac catttttggc gacgtggtca caaatcccct gacagccagc agcaacacca
    12061 ttatcggtaa cctgttcatg gtgtttaacg caattatcgc cattatgggg atagtctggt
    12121 tcctgtttat tggaatccgc catgttgtcc gcaccggcca ccagggcaat gtctttaaca
    12181 acggacggga cgtcgtcgga atgctcagta ttgtgagcgg ttttatgatg attgtgccca
    12241 ccgcttccgg ctggagcctg gcacaactgg tcatgctctg gggcgcatcc atcatgggca
    12301 ttggttccgc caatatcatg gttcagacgg ctgcagaaaa catcgctgaa ggctattcaa
    12361 tgaccgtaca gccagttcat gtctccaccc gcacggcagc acggggaata ctggagatgg
    12421 cgctgtgcaa acacgccatg aatgcaggcc tcgatgactt taaccgtact gcccgttcat
    12481 caacgccccc catgagcgaa tcctcacgga cagagaacgg caggtatacc gtcaccgttt
    12541 caaatggcag tgccacctgc gggagtgtga gtctcaccgt gaaagggaat ggcagcaaca
    12601 accagtccgc actggactat tttttcaatc ccttcattga aacggagtat cagggtgtcc
    12661 tgacggcaca acggtctgcc atggatacca tgctccgtga cctggacagc gcagcacagg
    12721 aatttgtgtc agcgtaccgg acccgacgcg acacgggtaa cggcaacctg ccggatattg
    12781 aaagccgtat tcagaaggct gccaacaggt atgaagaaac ggtgcagaag gcgttgccaa
    12841 cagataacgc agaaactgag cgcagggagc agctgaaatc ctacctgacc acctacggct
    12901 gggttgccct gggggcctgg taccagacct ttgccactgc caaccagcga ctggcagaac
    12961 tggccgaccg tgcccccact gtgagtggag tttcatcgca gggcgaagca ggggatactg
    13021 aattcctgaa ggaagtaaaa ggagcttacc aggcacagtt gcaaaactca ccatatacac
    13081 ctgcactggg aacagccacc tcagccagtg aataccaggc atctaatact caagatcctc
    13141 aatcagcaat cttaaaactg ataggattta acctaacaag aatgactaac tggacagcta
    13201 cttatctgag cggtacaggt actacaacta atcaactaaa cccgttgata aaaatgaaaa
    13261 atattggtga ttacaccatg gtcacaaccg aaaccatctg ggcgggttat accaccgtca
    13321 gggtgctttc cactgccggc agcaactcca tttttggtcg actggtcaat gctgttagcg
    13381 gcttatctga tgccgcaaag gcttttctgg aagcagctgc accaccgctc tacttcctgc
    13441 tgtttttgct gttctgtgcc ggtttttcgt tatccattta cctgccgttt attccgttta
    13501 tcttctggat gaccggcatc ggtaactgga tagtcagtgt actgattgga tgcgccgccg
    13561 gccccctgtg ggccgcaacc cacctaggaa caagtcagga cagaggaagt cgggccgctt
    13621 acggttacat ctacctgata gacagcatga tcaggccact gctgatggtg ttcgggttct
    13681 tttttgcatc ggtcgccatc gtggctggcg gcaccatact gaatgctctg tacgctgcag
    13741 cactaattaa tgtccagata aattctgtga cgggggtgtt cagcatggcg ggatttttga
    13801 tgatttatgc ccgcgcctgt accacactgg ttgcgggcgt ttttgcactt caggcttatc
    13861 tgccagacca tgtgattaat tttctgggtg gacgtgacgg cgttaatatg ctggggaata
    13921 tggtttcttc cgtcaaagat attattgccg gcggaaacac caatatacgc agaacacctg
    13981 gcgttcgtga agaaaaactg aaaaatatta atggagatga taaggacgga ataaaacaat
    14041 agagtacaaa acagtattgc catattcacc tgaagagatt aaaatacatt caggtgaata
    14101 tgggggttca ttatgaatac aacaaaaaac agatatcctt cctggtgggg atattattat
    14161 cttattcgca acacttactt tatatttggc attccttttt tattgttatg ttctctaggc
    14221 tttaccggca tgatgcttac agaaccatat ataggaacag atattcaagc atatctatat
    14281 acttgtggca gttggtttct attaataatt ccttcactat ggctatatct gagaactaag
    14341 gcaaagaaaa agaaaatcct acaggttgtt aagaagataa aagaaagcag tccctttgcg
    14401 ccgactagcg attacgagca actgtcattc tcgaagagtt gttatttcgg gatcgacatt
    14461 aaaaacggca ccatgctgta tgtgcgcatt tacccaaaca atgtcatgga tgtgattggt
    14521 ctggatattc acaacttcac ccgaacagtt gctgaagacg gcaagctgga aatccacacc
    14581 acctatgtga gtctgccgat gatccctctt gaaatctccg gcatctcatc aaggacactg
    14641 gccaacacca tgcacaccat ggctgcacgt ggatatgagt acaaggaacg tttcccgcag
    14701 atgatccggt atcgggtcaa agagtgggaa aaagttgccg gtgtgcctgt tgcagaagta
    14761 ttttaaggta gcgaccaact gttagacggt aatccgtcag ccggttttct ctgaaccggc
    14821 tttttctttt caggcagaat gcctgtctgc cgtctatctc aggacgccgc atggagtctc
    14881 tattttttcc ccacaaattc ttagcactgt cttgcccact gtagacaaaa tcaatacaat
    14941 tctgcatatc agttatctat aaaagataac aatatcatta taaataatta tcatatccac
    15001 acactcaagg cagtacctag aaaacataaa gataaaatca tgtctgaatc ccgcagcaat
    15061 tttccagcac gacatggtac tccatggaat actgaagagt tacagcgtct tctcgtatcc
    15121 atagcggagg gcgtctcgct aaaaaaactt actcaaacac atcaacgatc gtccggggga
    15181 atacgtggag caatcaaacg cctgcttcct tctgagtttc ttgagttcaa cgatataaac
    15241 actgtgaatg atctcagcaa ttacttcaac aagattcctc gccaagaaca caatagtatc
    15301 atcaataatt taatatcatt gagcactgca aaaacaattg ctcatgataa agttgaaata
    15361 caaaagaata gttttaaaca aaatacccca ctccccctct gccaacccaa tacagatttt
    15421 aaagattata tagccgatga acatgatgtc attatgctaa ttcattccgc tatcgaaagt
    15481 ttatctaata aacgacatca atatattctt ctaatgcgac tcggcacaga tgattcacct
    15541 cattctctgg cagatattgc cgtcaaatta ggaatttccc gtgaaagggt aagacaactt
    15601 caggaacgag cccttggctt actagctgct aaatctcgct atgaagggac accaggagct
    15661 tgccttaaag cccttattaa ctcaataggt aattcaccat atgatatagc agtatggatt
    15721 tatcgtattg ttcatcggga tttcgttaca tcctctgagt tagccgccaa attcattatt
    15781 tttaccgcag gcattcctaa agcaagacga gatgaaataa aaaattcgct ctcattaata
    15841 tcacaattac aaaagcagag gttacgtgaa cgaagagcag aattagcaca cgctcgcgct
    15901 caggaaagag cccgaatcaa gaaagaaaga gttgagttca tattcagtaa atggctagac
    15961 aatacacatt ggccaaaata tatcgcacct cctccccccg tagaacagct atgttcacag
    16021 agagcggtta atgatactga catatctgga ttcttttact ctcaaaaatt aggtcgtaca
    16081 gtccagtacg aatcagggct tgagtttaaa ataatgaatc tattagaatg ttgcgatcag
    16141 gttcttttct atcaagaaca accattttct attccatatt gttttaaaaa caaatcaaaa
    16201 aaatactatc cagatttact cattgcgaca gttgatggtc ggtgcctact tatggaagtg
    16261 aagccaacag accgaatgac cttgagtctc acgagaataa aaagtaatgc aggtcgcaaa
    16321 tgggcacata ctcgaggatg gggatggttg gtcattagtg ataggttctc gttacgtcag
    16381 ctggaagaac atgccatctc aacaaacaca tggaaactta tagaggctga actgaaaaca
    16441 tcaaggattt tagcatggcg tgaaatgatg gagttacgta cacgatacga gattcatcgt
    16501 ttagatctta ttgcatatat cattcagtct ggtgctgaag tggataactt ttatcgaatt
    16561 accccgaaaa tatctaataa tacatctaac cagagacaac caaatgagag cgactgtcat
    16621 aattgatgac gcacaggacg gaaaaaataa tagctgccgg acagcagcaa ccagcagtcg
    16681 tccggtaaaa aagacaaaca aggcgcattg aggtcagcct tcgcaacaat ccggcggcag
    16741 atttcctgcc gccgtgttgc ccgttaacgt ttaacttcgt aggctaacgt tgccacaagc
    16801 actgtgtttc cctgctcgac acggaatccg caaagcgaat ccctcagcat ccagacaaaa
    16861 cacagtatcg tcacacagac gacaattaac attgttaaaa acgttcgttg tggcatcgca
    16921 gcccctccat tgcctttcgg ccgtcatcgg gctactataa tgttgtctag gcattacagt
    16981 gggccctggg ttgattgata aaaaatcgcc tggggctttc tgctgcatgc cgtccacaaa
    17041 acgctttcag catacagcct caagcgcccg ccgctattct aatcgatttt cagtctgcca
    17101 caatcgtttt ccacctaatg acatgttatg cacaacagta tttttacggg aaactacacg
    17161 gcaaggattc ccggaacacg ttcattattt tatggttaca gacatcatgt taaatccgga
    17221 agtaataatg acctggaagg aaatactatg agtaacgaat atcaaagcac cagtataaac
    17281 ccggatgttt ttgaacaatt ttttaccctc ctgaatacac actgtaaatc atcagaagct
    17341 gcacttgcca tgctgagtac actctgctac gaactgatga ggaggagtgg gatccattca
    17401 ctggacagcc tgatgctcaa cggagaaggt atattgatac aattacaggg ggctgatatt
    17461 cgcgaaaaaa caggtcagca tattcactga tccagggatg tgctgaaaac agtgtcagcc
    17521 tgaaataaac gttcaatgcc tcatacacat gaggcattga gcacggtcat aaataatctt
    17581 tattattttt ctgattcagc atttccggtc agtatcggct cttctggcaa agacattacc
    17641 ggaacgtcac gttcaccatt gaatacagaa acactttcag cgaaatcatt ctgtaacaaa
    17701 gaaaaactta acggcagatt tttggtttgt atccctgtga tccattgaaa aatctcggga
    17761 aaacgacgtg cactgatatg cacatgattg aggtggtgct cagtaatatc actggccatc
    17821 atcatgagag gtacatcgta tcccgaacgc aaacctggcg catgatgcac aggggtgctg
    17881 tctgaactga cggattgccc atgatcagaa aaatacagca tggcaaaatc atcaccatgc
    17941 tctgtcgtca tgttataaat tgcctgaaga aaccgatcca gcttaagcaa tgttgataaa
    18001 taacaggata ctttatttcc gaataattcc ctgaatggct gtgcatcatt aaaaagtcgg
    18061 tcacagggat tagtgtgaga ccccagcata tgaataaaaa tcacttttgg agaagacgag
    18121 ttaacagctt ccctgacata ctgaagcata tcctcatcat caatgcgaag ggaagaagag
    18181 ctcccttcct taagaaaatg ttttttatcc gcattggcgg cgatgacaga aatcagcgta
    18241 tcatgttgcc ctaaagagcc ctgatttgat atccaccacg tctcataccc ggcttttctg
    18301 gcaagcgtaa caatattatt acccggattc cctgtttcag tgtaatcaag cgtcagtgtt
    18361 ctgctgagtg aacctatcgt attcggcgct gctgagaaat acccattaat aaatataccc
    18421 ggagcagaat tcagccatgg tgttgtcggc aaaggatagc cataaacaga aagataatcc
    18481 cgtctgacac tttcaccaat tacgataatt atggttttat atttcttctc cacactcaca
    18541 atatcccatg agtctttctg attgtttttc agcgattcat atcgcatcat ttcttctctg
    18601 gtttcagcta tccccgtcat tacattcgta accatacgtt ttggccatgc atttactgac
    18661 aatacgataa agaacaacag aaagataaat ccccggtggc ttttatcacg cttacgccag
    18721 aaccagaatg taaaagcagc aatgaccagc gaaacaataa aataaagaac aggtatgcct
    18781 gtgataaact cacctgcctc atttatattt gtctgcatta cagacgcaat aaaactgttg
    18841 tttattttcc cgtaaatcat cccaaccgga gcatagcatg cacttaacaa aagaaagata
    18901 cagacacacg ttgctgaaat aacattctgc cgccccaaca acaacatcag gaacaatata
    18961 agcacattcc ccagggtgtt tttcactgta tatccgcagc accatgtaac aagcacagaa
    19021 aacaacagat aaatcagtga ctctttgcac cacaacataa aataaaactg ttttgctctc
    19081 tcaaaagcat attttgattt ttctttaata caagacacgc cagtcatttt cctgtacaaa
    19141 cacagacaat catttcaacg catggatatt tatatgaaac agctcacaaa aacatcctgt
    19201 tctgtccagc aagattgaca tattatcagg ttctgtgttt acagcaactg ctccccgaac
    19261 cagattattc gcataacccg atgaaggcct cataaccata aaacactcag aatacattta
    19321 gttaacaaaa atatgtcttg ccctcccgga aaggcaaaca tacgaaagag aaagcatggc
    19381 tataataata tcaatcaaaa tatactcaat ctttccggtt gtcatacact cagcaccgac
    19441 aacataaaga caataaacaa aagtgatagc atagtgcgat atataaagga gatgaatagc
    19501 cagaagtaat tttcctacca ctctttttcg tttaaagaaa gaataaatga atagcaaaca
    19561 taaccctgac agaaaaagaa aggtagcaat ggagattata aaatactccg tcacctgtga
    19621 attctcaccc agagatgaat acagcgaata atatgtagtt gtgagattaa gtcttgttat
    19681 caatatataa acaactgata atagtataat ataaagaatc agaccaaatc tgtttttctg
    19741 cgctccggtt aatacttctg atttatctcg cataaaatat cctgtctgta atctgtcccg
    19801 gtaaacaatg gtaaatttca gaatcagtgc gaatacggta acaaagattc ctcctgtgct
    19861 ttaaagtgtg taggcagaaa ccagtctggt ggttccggtc agggagtctg atacctcaac
    19921 ttcccgggtg actatcacca tacctgactg acgcagtctc tgcacatcag cggcaattcg
    19981 ctgagttccc agccagaaca ggccatccag tgccgttacc agcaacccgc tttccagcgc
    20041 cagtttcagc cgtgccgtcg ggactttaac ctgcccggcg atatcagtaa aacaacgcgt
    20101 cgtcatgcca tagcagtcca tccgccgtat tcgcgtcgca agactgtagc tgaaacaatc
    20161 cggcacgccg gataagtcat tctgcagact gtatacacat ccccgctgcc gcccgttgaa
    20221 cagggctttt ttctgactga gcaaccattc ccgacgcaga gtgtcaatca tctgcggtgt
    20281 ccgggttaac agcagacgaa acggcagctc aaaactggtc acataatcaa cattcagtaa
    20341 tgctgtccgc agtcgctccc tggcacctgc cccggtctcc cggcattcac gcatgacatc
    20401 agcccactga cgcatcagtc gtggagactc attcctggcc ctgaactgat atttctcaat
    20461 atgataatca atacgttcct gcatatcgcc accagatatc tgttgtttac ccagacacag
    20521 tgattagcct acaggaataa atatcctccg tgaagccagg tgcctcagaa gataaaaaca
    20581 tgtatttttt cgacgcgtaa tattaacgtc atccccataa aaataaatca ggaaaatata
    20641 aaatgataaa atatatattc gcactcactc ttctgctttc cggttctgtc tgtgccgttc
    20701 aaattcaggg actcgttatt cgtgtgctgg acggtgatac tctggagata ttacaggccc
    20761 ggcaccccgt gaggattcgt cttgtgaata ttgatgcgcc ggaaaagaaa cagccttttg
    20821 gacgctggtc aatgaatcaa cttaaaagcc ttgtggcaaa tacgcccgta accgtgacat
    20881 acacacagac agaccgctac gggcgtattc tgggacgggt atataccgaa gacggcatgg
    20941 aagttaaccg ctggctggta cagcatggtg cagcctgggt atatcctgat ttcaacacag
    21001 attacaccct gccggcatac cagagagagg ccagaacaat gaagcggggt ctgtgggcag
    21061 ataaaaaacc tgttcctcca tggctgtggc gaaaagaaaa taaaaatgac cacacagaat
    21121 tacaaataaa caacatctta ccgtaatatg tattttttct gctgcattat cttccctgtt
    21181 tacagtcagc aaaaaaacgt ccgaagcaaa acaaacacga ataataaacc tgcccgatgc
    21241 atccattaca aaaatgaatc cttcctctta tggaaaatac agtaaccaca atgaaaaata
    21301 aacagataaa gatgatactt atctcacaag gaaaagcccc ggcaaaaaca taagattcac
    21361 ttaacttttt caccttgtag aatcttcttt tttcatggac atgttatttt gttgatttta
    21421 aaatatcaaa atgggtgaat gtatcacatc actcataaag tggatattat cacgaaacta
    21481 tctattaaac agcagattag cagatacaac atgaataaaa tttactcact aaaatatagt
    21541 agccttactg gtgggcttat agctgtatca gagttaagta aaaaagttaa aggaaaaaca
    21601 ggcagaaaat taatgactgc ttccgttgct ttatctgtga gtctttcagc cttacccgca
    21661 gaggcatcaa cggtcagcgc tgaaatacca tatcagactt tccgtgattt tgctgaaaat
    21721 aaaggcgtat ttacacctgg tgcaaccgga attgaaataa aagacaaaaa tggaaatgca
    21781 gttggtacgc ttgatgtgcc aatgattgac ttcagcagcg tgtctcggcg gggctcactg
    21841 accctgttgt cgcagggata tggtgtatca gcaaaacacg gaggccttgg tgatgtaaac
    21901 aatgccagtt ttggctatga taaaaataat tatactgtag taaagaacaa caaacattcg
    21961 ggccttgatt tttctctgca tcggttcagc aaactcatca cagaggctgc acctgctgat
    22021 ataaatatat ccggacaact tagtgattcg tcacagtata ctgcttttta cagagcaggc
    22081 gctggcacac agtatattaa agaacgctca ggtaaacaga ctcatatccc gggaacattt
    22141 ttaactggtg gaacagtagg caccccatgg tattcaggaa ataatttaat atcaagctct
    22201 ccaggtgaca cctataataa atcacaggga ccattagcca gttatgggca gatgggtgac
    22261 agcgggtcgc cattatttgc ttatgattct ctcagtgaaa aatggtctct ggctggagtc
    22321 accctgcata ataatggtgt taatggccaa aaaaacaact ggttgttatt acctgaagat
    22381 tatattaaaa atattattac agccgatttc gatcccatta tttcatttaa taaaaacagt
    22441 aaagaacata tgtcatggac atatgatgca gccaaaggcg tgggccgtat tcagcaggat
    22501 gaccaacagt ttgtgatgca tggtaacctg aatggtaatc tgaatgcagg taaaaatctt
    22561 tattttacag gcgaaaatgg cattattgat ttaaaagata atgttaatca gggggccgga
    22621 tatcttcagt ttgctgatga ttatacagta acaacgagca atgacagttc atggtccgga
    22681 ggaggcatta ttgtcaatta tggtacaact gtaaaatggg gtataaacgg ggtttccgga
    22741 gatgaccttc ataaagttgg ggatggaacg cttattatta atggcacagg gaaaaatgaa
    22801 gggggactga agattggggc tggtactgta atccttgaac aaaaagcgaa aaacaacgat
    22861 tcaacggctt tttcttccat taacatcagt gggggaaact cgcgtgttaa actctcagga
    22921 gataatcaga ttatacctga taatgtttcc tggggattcc ggggtggata tttagatata
    22981 aacggaaaaa acacagagtt ctcccgctta caggctgttg attatggggc tgcaattatt
    23041 aacagcagca cagacaagtc attattgact ttaaatctgt caccattgaa aaaagatgaa
    23101 atagctgtaa gcgtgaaggc gttagatatg aacgccatct ttcagggggg acacgggaca
    23161 gccggagatt tgtataaaac aactttttac ggtccgacac agtattatct gctgaaaaag
    23221 cccaaatttg gcagtgtgct gatggggtca ctgaaaaaca ccagcgaatg gcaatttgca
    23281 ggaacagacc tgaatcaggc cgttgatatg gcaaagaata ataaactgac atcaagtgca
    23341 caggcttcat atctttatca cggtaagctc ctggggaata tggatattgt cattccggag
    23401 cttacaggca atgatatttt aacgcttgat ggttctgtca gtatatcagg agatatgtca
    23461 aaacaggacg gtgctcttat cttccaggga cacccggtta ttcatgcagg gcaaactgtt
    23521 tctgcatcgc agagtgactg ggagaacagg gagttctcac tcaacaatct gaatcttaat
    23581 aatgcggact tcagtctgtc ccgtaatgca tttatgaacg ggaatatcag ggccgttaac
    23641 cagagcactg ttattatcgg cggagataca gtctttactg ataaaaatga cggaacaggt
    23701 aatgatgtca tcagtgttga agggaaatct gctgccgcag gaacatcctc ctatacaggg
    23761 catatcactc tggagcaaaa atcagcactg gatatccgcg ataattttcg tggcggggtt
    23821 acgtctgaag acagtcatat caatgtttct tcatcttcag tcctgttctc agatgcatcg
    23881 tcatttataa acagctccct gaatattcat aaaggaggag cgctgaccgc tcagggaggg
    23941 ctgtttacaa gtggaagcat tgatattggt gacgcttccc ttctgcttac cggtacacca
    24001 gtgaattcag atgatgctgc ttttttaccg accatcaata tggctgatgg cggatttaac
    24061 ctgatgtctg attcatcagt actgaaagcc agagaccagg catctgttgt tggtgatatt
    24121 atttctgata aacaggccac aatcagcttc ggaactgaat caggtaaaga gggcatattg
    24181 tctgagaagg catcccgggg actggcggtc ggattactga gtggttttaa tacggcatac
    24241 cgcggtgcaa ttcatgcccc gtctgcatct gccactgtga acaatacctg gtggcaactg
    24301 acaggagact cctcacttcg ctcgttaaaa aataccggaa gcatgacata ttttacagga
    24361 agtgcagcga ataaagcatt ggatttgccc ctatatttcc agacacctgt tatcacttaa
    24421 cccattacaa gcccgctgcc gtagatattc ccgtggcgag cgataaccca gtgcactatg
    24481 cggatgccat tcgttataat gctcgaacgc ctctgcaagg ttctttgctg ccgttaaccc
    24541 gtctggtttg ggcatgacac tgatgtagtc acgctttatc gttttcacga agctctctgc
    24601 tatgccgtta ctctccggac tccgcaccgc cgtgctcttc ggttcaagcc ccaacatccg
    24661 ggcaaactgc cgtgtttcat tagcccggta gcatgaacca ttatccgtca gccactctac
    24721 tggagacgcc ggaagctcgt tgccgaagcg gcgttccacc gctcccagca tgacgtcctg
    24781 tactgtttca ctgttgaagc cgccggtagt gaccgcccag tgtagtgcct cacggtcaca
    24841 gcagtccagc gcgaacgtga ctcgcagttt ttctccgtta tcacagcgga actcgaaccc
    24901 gtcagagcac catcgctgat tactttcttt cacagccact ctgcctgtat gtgcccgttt
    24961 cgatggcggt acagcaggtt ttcgctcaag caacagcgca ttctggcgca tgatccggta
    25021 aacacgtttg gcattgatcg caggcatacc atcaagttct gcctgtctgc gaagcagcgc
    25081 ccatacccga cgataaccat acgtgggcag ctctccgata acatggtgta tacggagaag
    25141 cacatccgta tcatcagtgt gacgactgcg gcggccatcc atccagtcat cggttcgtct
    25201 gagaatgacg tgcaactgcg cacgcgacac ccggagacaa cggctgacta agcttactcc
    25261 ccatccccgg gcaataaggg cgcgtgcgct atccactttt ttgcccgtcc atattcaacg
    25321 gcttctttga ggagttcatt ttccatcgtt ttcttgccga gcaggcgctg gagttcttta
    25381 atctgcttca tggcggcagc aagttcagag gcaggaacaa cctgttctcc ggcggcgaca
    25441 gcagtaagac ttccttcctg gtattgctta cgccagagaa ataactggct ggctgctaca
    25501 ccatgttgcc gggcaacgag ggagaccgtc atccccggtt caaagctctg ctgaacaatt
    25561 gcgatctttt cctgtgtggt acgccgtctg cgtttctccg gccctaagac atcaatcatc
    25621 tgttctccaa tgactagtct aaaaactagt attaagacta tcacttattt aagtgatact
    25681 ggttgtctgg agattcaggg ggccagtcta agcattccat acactgacgg ttgatgagct
    25741 gacgacgaat ggcactgcgt atgccatgcg tacggacctg aaaaatgcgg ataagctggt
    25801 agtaaacaaa aagctgtcag gtaaggacaa tattctgttg gttgattttc tgaacaaacc
    25861 ctccggagaa aaactggata ttgaactggt gagtgcaccg gggaacagca gtaaagatgt
    25921 ctttaaagga agtgaacagg cgataggttt cagtaatgta acgccggtca ttacaacccg
    25981 ggaaaccgat gacaaaataa catggtcact gacaggctat aacacggttg caaacaagga
    26041 agcaacccgg aatgccgccg ccctgttctc tgttgactat aaagcgtttc tgaacgaggt
    26101 caacaacctg aacaaacgta tgggggacct gcgtgacatc aacggcgaag ccggtgcatg
    26161 ggcacgcatc atgagcggta ccggctctgc cagtggtggt ttcagtgaca actacacgca
    26221 cgttcaggtc ggggtcgaca aaaaacatga gctggacgga ctggatttgt ttaccggttt
    26281 cactgtcaca cacactgaca gcagtgcctc cgccgatgtt ttcagtggta aaacgaagtc
    26341 tgtgggggct ggcctgtatg cttccgccat gtttgattcc ggtgcctata tcgacctgat
    26401 tggcaagtat gttcaccatg ataatgagta cactgcaacc tttgccggac tcggaacccg
    26461 tgattacagc acgcattcat ggtatgccgg tgcagaagcg ggctaccgct atcatgtcac
    26521 tgaggatgcc tggattgagc cacaggctga gctggtttac ggttctgtat ccggtaaaca
    26581 gtttgcatgg aaggaccagg gaatgcatct gtccatgaag gacaaggact acaatccgct
    26641 gattggccga actggtgtgg atgtgggtaa atccttctct ggtaaggact ggaaagtgac
    26701 agcccgtgcc ggtctgggct accagttcga cctgctggct aacggcgaaa ccgtattgcg
    26761 ggatgcatct ggtgaaaaac gcatcaaagg tgaaaaggac agccgtatgc tgatgtccgt
    26821 tggcctgaat gcagaaatca gggataacgt ccgctttgga ctggagtttg agaaatccgc
    26881 ctttggtaag tacaacgttg ataatgcagt caacgctaac ttccgttact cgttctgata
    26941 ctccgtattc aaaagggcct gcgggccctt tacgctcagt gcctgccgcc ctgatatgtc
    27001 tgagagacat ttccgggatg tctgtcgact ggtttactta actgcgatgg ttaaagctgg
    27061 cagtaatgcc ggctttttta cggtcgcaca cgtcttcctc tcatccggaa ctgtgataca
    27121 aatcctgcat acaggattga ccgccggttc ctgcaaatgg cacattttca gtaagatagc
    27181 gaaggttaca ttcccccgtc atgtgtggca cagctgtcac cgggaaagaa acggaaagca
    27241 catgaaaaac atcaaaactc acaccggact ccttatccac aaagaacaaa cgcggcgcgt
    27301 tcgtcttcat gaaacaccca ctgcctggtg ccacacgcac cgggaatgct acagcaaaac
    27361 gaccggtcgc cgctgcggca gccctgacag tctcagcagg ctgattctga gcagcattca
    27421 gcccatagaa tgagataacc cctatgaatg atggcagaca gacgcctccg ccccccgagg
    27481 catggaaaat ccggtttctg gcgaacggta cacccggcac atggttttac accgatgacg
    27541 cagccgtcac aaaagaatgg ctgcgggcac attgtgacga tgtggaagtg atccccctgt
    27601 gccgtatgca tgacggtccc gttttccggg aacaccatat aaatgaactc agcgccgcac
    27661 tggccagaat gctccatatg caccacatac tgctggaacg aacccggtac agagaaaata
    27721 tccatgatgc agaatgtatt cgtgaaatcc acgaagcccc cctgcaggct atcggcatcc
    27781 tggaaaaata caaacgcaca aaataataag gctgttctat gtggatcctg attttctggc
    27841 tctcatcccc ctttaatgtc tcctccggcg atgcccggca acccacactg gctgttcaca
    27901 gtcaggcgtt ccgtactgaa aaagcctgtc gggatgcctt caccgcgctg aaaaaagaaa
    27961 acgtggggga atttgctctc cgtggcgtct gcgtccccgc cggagaataa acaagacatg
    28021 cgcaaccgga gtccgctatg acatccccgg accttataca agctgatctg caccgaatgt
    28081 cctggtctca gctggcaaaa gccgcggaag agtcgacagt acatcatgat tatgcccggg
    28141 cgttaatatt gtggcggcat gcatatcatg ccgccacgct gactattaat aaaaacctgg
    28201 caaccgctaa aatcaacttc tgtgctaagc gcattctgat gcgtaatcaa atgagcaaaa
    28261 taatcagaca tacagacaca gacgaaagac tcttccgtct gagtaaacac catcatttat
    28321 atgagaaaaa gaaaacaaaa gagggatgac aatcccatga tatgggaaaa acttgccatg
    28381 cttactgcag gtgcattttt gatactggca gtcctgtggg ggtaataata tcctgtttat
    28441 taaagggcag aaggcggtgc ccctctgccc ttaagtacgt ttccgggatt atcagtcaat
    28501 aagttcccgg atcagttcca gaagagctat cactgcattg accacagcaa caactgccct
    28561 gagaacgatg attaacaggt taatcatccg tttctccgtc aatggaggaa atcactgttg
    28621 tttactctta cattgcctgt cacaacagct ctttcattgt tagatgtaaa gtgatttctc
    28681 tccagccaga gcactgaaca cagcaccacc tgttcgatca gccaggactt aaaatctcgc
    28741 gttcatcgtc ccattggaga tcacggcaga agcatcacca gacagagcta acggagcgat
    28801 tatagtcatt tttctgattg caaaaaagag aagcgcagcc tactatgatg gtgttagatg
    28861 tagagtgatt tccctttcgg tcagagtcac caactccgcc ccggaatcgc aaaaaaaccg
    28921 acctcaccag tcggtttttt tgtgcctgtc tctcactgcc ttctgctgtt cctgtgaaaa
    28981 taccctggtt atttcatccc tgcccggtca ttaccggcga ttgttcgcat taattccact
    29041 cagcctcatc ccgaaattta tctatttatc gatggaggaa tccccgtgtc ttaccaacag
    29101 acgtctgccg ccgaagatcc aatggctata tggtatatcg tcggtgccat atgcctgctc
    29161 tttgcgatta tcatctggcg ctttttacct gaaatcgtct tcgcctcctg cctgatcctg
    29221 cacacactgt ggggcgtgat tgactggggg ccctttcaca actttgccgc cccccgttac
    29281 aacctgctgg ccatcactgc taacaacgcg gcaaccatca ctttcagcca gtggctggat
    29341 gttatgtccc ggacagtggg gatcctctgg ttgattctgc ttcccatgac attcggcttt
    29401 ctgtggatgt ggtttcacca tccggcccag ccccgtttta cccgcaggcc actgaatatc
    29461 cacacactgc cccatatttt ttcagctctg tcaccggcga tcgcccccgt tctggctgat
    29521 ggagacaaca acagactctt ccacggacaa aaacgtcccg agcgccgtgt cgcactaacc
    29581 cccgaagcct ttgtggagca gaacaacctt atccgcaaca tgcagctcga tgttgcgtcc
    29641 acacgccagt gcttcatggc gcaactgggg caaccactga ccagctggaa agacatggcc
    29701 ccacatgaaa aagcgctctt tgccatcttt ggcctgcagt tcttcctcgg tgaccggaaa
    29761 gcagcggtag ccctgatgaa caacctcaat ctctcctgca ggctcaaaag taaacgggac
    29821 caggggcgtt tcagcacgcc ggtgtattca ctggccagaa acgcctttat ccgggtcatc
    29881 aaaacagaag gcgcacaaaa gtggctcagg cagcaccgtt acgttcgcag cggtctggtc
    29941 tggctttacg cacatgattt gcgcctgacg cctccgaact ggctgtggct caagggcgtt
    30001 gaccggacgc tcttctatgc ccttcaccgg gcaaacacca caaaaggctt catcgaaggg
    30061 gccggcgtgg tggccgttgc ccgggcagaa aatgaagcgt gtcgtctcgg cctgccctgc
    30121 ccggaaccct gtgtggaaga agccattgaa gggctgcggc aggacatgct gagactgggg
    30181 ttgatatggg acgaacctca gccagaccgc gatcgcagac gtcagataag gacccgctgg
    30241 tcactcacgg atgacgtgat accacgccgc catgataacg atgaaggctc agatactggt
    30301 gaaaccaccg aaaccactga aaccactgaa acccggcatc ccgccgacaa ggaaaaagca
    30361 caatgaccat ccgctacact gcaacactga acaataaaca cctgtccctg accgccctgt
    30421 accctgacgg tggtcaggag gttattgcca tcctgccggt tacagaactg ggcgcactga
    30481 ttaaccgtaa taacaggctg atgcacctgt ccttcagcac cccttttcgt gaaataaccg
    30541 aaacactgct tgttgatacc gttgatactg aacctcccgg acatcacatc ggcagaatgc
    30601 tctccgcgat tgacagctgt ctgagcgcgg catatgcaga acttcctgcc cggaaaacca
    30661 gcttcatgag agggctgata tacggtgcag gattagtgct ggcacttctg acagcggtaa
    30721 cagccggata tattttactg aaaccggaca gcgattcccc tctcagggta cctgtcacag
    30781 aatccgtaac agaaacagca ccattacaca tcgacaagga aattgtccgg tcagtagccc
    30841 cgtcagagac gaaaaatgaa atcgttcacc cggcaccaca ggaaatgcag gagacatccg
    30901 ctaccccggc gataacagca gacaaaaata ctctggtcgc tgaagcccgc cgtaatctgg
    30961 ccactgtact taaacgcaac gcggaccggg gtatgttcac cattaatctc tccactggtc
    31021 atgaacgcac actctacgcc ttccttgacc ccgcatgcat caactgccgt cttctggagc
    31081 ccgcgcttaa gcaactggcc cgggaattca gcgtggtgat ttatccggtc tccgtaatcg
    31141 gcggagaggc cagcacaaac cggactgcgc cactgctgtg tgaaccggac gcacaaaaac
    31201 gggcgaccgg atggcatcag ctgtattccg cagacggtgg tatggcaccc accggcgatg
    31261 caccggctgc accggatgaa aagtgtctga ccgctgctcg tgctgccatc gatgtcaata
    31321 accaggcttt ccgccagttc ggttttggcg gtaccccatg ggtgttatcc gataccggct
    31381 ggcacctgcc gtccggcatc ctgaaagacg ccactaccgt cagcctgttc ctgaaagcca
    31441 cagataccga ggataatcac aatgagccag catcacgtta atcctgacct catacaccgc
    31501 acggcctggg gaaacccgct gtggaatgca ctgcacaacc tgaacatcat tggtctgtgt
    31561 ctggccggca gtatcattac ggccctgata tggccactgg cccttcctgt ctgtctgctg
    31621 ttcaccctgg tcacctccgt gatattcacg ctgcaacgct ggcgatgccc cctgcgtatg
    31681 cccatgacgc tcagccttga tgacccctcc caggacagaa aggtcaggcg aagcctcttc
    31741 agcttctggc ccaccctgtt tcagtatgaa gcagatgaaa catcccccgc acgggggatt
    31801 ttttatgtcg gttaccgccg tatcaatgat atcggtcgtg agctctggct gagcatggat
    31861 gacctcaccc ggcatgtcat gttttttgcc accaccggtg gcggtaaaac agaaaccacc
    31921 tttgcctggc tgctcaaccc gctcagctgg ggaagaggct ttacctttgt tgacggaaag
    31981 gcacagaacg ataccacgag gacaatctgg tatctctccc gacgttttgg ccgggaggat
    32041 gatatcgagg tcatcaactt catgaacggc ggaaaatccc gcagtgaaat cattcagagc
    32101 ggcgaaaaat cccgcccaca gtcaaacacc tggaacccgt tcgcattcag cacagaagcc
    32161 ttcaccgccg aaaccatgca gtccatgctt ccccagaacg ttcagggggg agaatggcag
    32221 tcacgggcga tagccatgaa caaagccctg gtctttggca caaaattctg gtgcgtccgg
    32281 gaaagaaaaa ccatgtccct gcagatgtta cgggagcata tgacgctgga aggtatggca
    32341 aaactctatt gccgtgggct tgatgaccag tggccggaag aagccatcgc accactgaga
    32401 aattacctgc aggacgttcc gggattcgat atgtcactgg tgagaacgcc gtcagcatgg
    32461 actgaggagc cccgtaaaca gcatgcttat ctctccggtc agttctcgga aacgttcaca
    32521 acctttgccg aaaccttcgg ggacgtcttt gcggcagatg ccggcgacat cgatattcgc
    32581 gacagtattc acagcgaccg cattctgatt gtgatgatcc ctgcactgga cacatcggcc
    32641 cataccacgt cggcactcgg tcgtatgttc gtcacccagc agagcatgat cctcgcccgt
    32701 gacctgggct accgcctgga aggcacagat gcacagaccc tggaagtcaa aaaatacaaa
    32761 ggcagcttcc cctatatctg tatccttgac gaagtcggtg cctactacac agaccgcatt
    32821 gctgtggagg ccacccaggt acgttcactg gagttttccc tgataatgat ggcgcaggat
    32881 caggagcgaa ttgaagggca gacctccgcc accaacaccg ccacactgat gcagaacgcc
    32941 ggcaccaaat tcgccggacg cattgtcagt gacgacaaaa cggcaagaac ggtgaaaaat
    33001 gccgccggtg aagaggctcg ggccagaatg ggcagcctgc agcgccacga tggtgtgatg
    33061 ggtgagtcct gggtggacgg taaccagatc accatccaga tggaaagcaa aatcgacgtg
    33121 caggatttga tcagactgaa tgccggtgaa ttctttaccg ttttccaggg ggatgtggtg
    33181 ccgtcagcct ctgtgtatat tccggacagc gagaaaagtt gcgacagtga tccggtggtc
    33241 atcaaccgct atatttccgt tgaagccccc cggctggagc gtctccgccg tctcgtcccc
    33301 aggacagtgc agcgcaggct ccccacaccg gagcatgtca gcagcatcat cggtgtactg
    33361 acagcaaaac catcccgtaa acgtcgtaaa aaccgtacag agccttaccg tatcattgat
    33421 acattccagc gccggttggc aacagcgcag acatcactgg atctgcttcc gcaatatgat
    33481 acagatatcg aatcccgggc aaacgaactg tggaaaaagg ctgtgcacac tatcaataac
    33541 acaaccaggg aagaacgccg ggtatgttat atcacactga atcgtcctga agaggaacac
    33601 tccggtcctg aagatatacc ttcagtgaag gcgattctga atactctgct ccccctggag
    33661 atgctgttac ctgtaccgga tctaactgca tccccgcccc ataaaaaaaa tgtcgctcag
    33721 acaccatcag gtggaaaaca agagagtcgt aaaaaaagat tctgataaca cctgtatggc
    33781 tgattttaat aacaacctta ataacaacct ttacgcgatt attaatctcc tccgctcacc
    33841 atagtaagcg gaggattcag gatttcaatc catcatcaga ggtacaataa atgtgtatta
    33901 gtagcccgga aacaaactca tggtctgtta tttatcgtaa aaattccgga gaagatatca
    33961 atatcacttc cctgacgttt aaaaatagtc ttctggcagc ccggatactg atggtgcccg
    34021 aaaactatat gatatgcata ctcagaaacg gggagagggt cagacgatgg gacagagaaa
    34081 tacttgcagg ctcaaacaga tggtacaagt gttctcccga taatttcgaa atactgggta
    34141 agctccccat aataaataaa gtcacaacac tgataaaatc ataatctact gattttactc
    34201 agttaagcta aatcaggatg aacgctttga ttatgaatta tacatgaaaa caaatatggg
    34261 cagcacaaaa aatggtgcca gggttcccca cgttcttaat agattcactt ctatggatat
    34321 tgatatgaat tcccagatcc tgccagacat gctgattaat tttgccctta ttaacatcac
    34381 agacagaaag aacgagggta ccaataccat tgatggtaac tggcaggccg atgaaggacg
    34441 ccgttaccgg gataatgtaa gaatctattt ttgagtatgt caacataacc tgaggcaaag
    34501 tacctcaggt tccagtgtag taatcagcca acctgacagt cttctgttag ccctacagat
    34561 atgtcgtgga gctaaccgcg catgtcaaag ccggattttt gaaaatctct ataaacttcc
    34621 ggattgttga aggccgggaa tttagcttta tgggctgctg actttatcgc ttcgcaatag
    34681 gctttatcgc cattactggt tgatattttt aacgccgtgc catcccggga gaattccata
    34741 tgcaacctgc attttttcct tttccatttt tgcggatcat caagtttagc attaattgca
    34801 tccctgattc cccgtgcctg ctccccccat tcatcctgat catcccaacg tcctgaactg
    34861 caactaccca gggctgtagt tttatggcaa tttgaaggat gtaatggcgc acatcccgcc
    34921 acaaagctga ctaaaatagc cagcataacg attttcttta cccccatata ttgctcctca
    34981 atccatatag atctcagtac catagctctg tcagagcaaa gaaaatctta actaatacaa
    35041 gttaagattt ttaatatcca ctcctcatcc ctacattcca ggtgatggtg gcgtccactc
    35101 attatcctga actggagcat aagatgcgcc atgccccccg gatatgcttt tctgttgcct
    35161 gatatattcg tggtagtact gttcactacg ctgacgatgt aaatccacat ccgggcggct
    35221 gattacaaag tgtccatttc cggttattcc agctgcatta tgccaggcca ccatttccgc
    35281 aaatacatca ttaaactgtt gatatgagcc ttccggttca aaacccattc ctggctcacg
    35341 atcaaacaat acaggagcaa tcagattcag cttatccacc agttcatttc tgtcctgatg
    35401 atggtagaac accacacggt ccccataatc cacacagact aactcgccat tcctccgatc
    35461 acgaaaacgc acactgccat ctttctgcag acgggcttcg agaacgccag tgtcttcata
    35521 aagtggagtt cctcccggtt cacagagaat cacgcagcgg tcggcattcg ttgttcgtcg
    35581 cttgtctttg cgacgatcac gataatccca gccacgtaac tgtgataatg cagctctgtc
    35641 tccctgtacc gcctgttgct ccacccactg ccggtaggac aaaggatacc acttaccctc
    35701 cgcaatcagt gacaaccgct cttccttcac actctccttc agcctgatca gcgcctgcat
    35761 acgctgcact tccgcaatat ggtaatgcag cttacgcaat tgtggatccc ggaactgcac
    35821 acggatatac gccttgcgcc gacggcaggc agcatgaatt tcccgtaacc gttctccgta
    35881 acgtaagtcg ggtttacgcc agtgctcctt ccatgcaaga taacgcgcgc gcaaatcttc
    35941 ccgcgcagct gcacgggctt cacggcgttc acgacgcagt acaggatcgc gccggaagcc
    36001 gggctccact tcatcgctgg cagcaagttc cggattgtag cgacattcag gcttcacccg
    36061 ttcaaatata tctgcacctg caatctcaaa tggccctgcc tgcggctcag ccctggataa
    36121 cgtcaaatcc gggtggatac tgctggcctt cactggtgtg agctcatgat taaacgcatc
    36181 cacaatcaca agaccatgat gctgtttctg caacataagc cctttacgcg caaacatctc
    36241 atggcacgcc tgccagctga aattcccgcc cgcgatcata tcttcaatca acgactcaca
    36301 ctgaacagtc gtgtaccccc acagactttc cttatcagaa aacagttcgc gctttattgc
    36361 tccctgtgga cctgtcccca tccgcatacg ataccattcg taatcactga cagcctgagt
    36421 accaagatta cgtccccgga atcgttctcc gggtataacc tgagtaaaaa tatccttcgg
    36481 tacaggctgc catccaccat ccagatcctg catataatcc agcgtcatta acggccacac
    36541 actgtctgca cgtactggtg tctgttgttt atcaaatcca tcatgcagta ccagatgccc
    36601 gtgctggatc cccatccata atccagcctt tgccagagtt ctgtgcacat ccaggcaact
    36661 ttccagctca ccatttctcg ccatttccgg taagcgttcg gcaaaatgac ggcaggcata
    36721 ctgtttcaga ctctccgttt ctgccacttt ttccggccgg atatcaacgt ttatggcttc
    36781 cggatcataa cgaccgggtg tccccacctg actgaaaata tctgtcggta caggctgata
    36841 ttcccccagt ttccgcccca gtttttcagc tgtccatgac ggtccgaacg aactgagtgc
    36901 aacaccttcc cgcgctctgt cccagccatc cttcaccacc agttcccctt cctgcatcgt
    36961 gatatacaga ccatcactgg ccagccgttt atgcaaagat aaccagtctt gtgcgggttc
    37021 ttcacgcagc ccggcaatcg acatctgcgc aatatattcc ctgaacgtct gctttttccc
    37081 gcgtcgccag gcattacggc gctcacgtac cagtgctgtt ttgcgcacaa tacggttgcc
    37141 gggtgcatgc acaaagcagc cattatccgg cgcgaatccg tgcttaagtt caagttcacg
    37201 acatgcacgg ctgagttttt cctggctcca ggaaagccag ttaatataac cagtttcagg
    37261 atgtacacga ttcacggcaa catgcacatg cagattatcc gtgtccgtat gaacggcagc
    37321 cacgtactga tgttttgaga gttcaagaga tttcagtgtg tgacgcacac agtcaaaaag
    37381 ctgctcagga cgcgggcttt catgcgcagg ccaggaaaga atgtagtgga aaacggggtc
    37441 tgtatcatta cgggaaaaaa cagctttgtc cgccttgtac tgcatctctt cagcagctgt
    37501 ttccagtgaa ttgcagttat ggaaacaggt tacgccatag aaatttaccc actgactgcc
    37561 gtctttcatc acatcaatca gggagacaaa tttttcgtta cgcaggcgtg ttgcataatc
    37621 aacaagccgt ctgaaacggt tgcggtgagg gacatcactc ttgatttcgc tgtcttctgt
    37681 gagctcattt tcctgcacat catcccgcac agacgtgtac gcaatcaggt cttcaaagga
    37741 agacctgccg tcacgtcttt tgtgcggaat aatggcattc atcagcagtt atctcagcgt
    37801 cccattaagt ctcttcgcag agcattgaca tattcagtaa tggcaacaag aacctctgca
    37861 tactccttat caccagtgcg ttttccctcc acaaacagat gcttctgaag gcggccaaga
    37921 cgcataatct catcaatcat acggtcgtca gtacggcagt taaccacctt atccagtgaa
    37981 gagcttcgta taaatgttga aaccgtctgt ccacttgact ccgctttttc tcttacgatt
    38041 tcagcctcat cctccgtgaa tctcgccgtg aattttacaa ttctcttcct gttctcactg
    38101 cccgttcggg ttttcttctc actcatggtc tgctccttac cggggtgtcg gggcaaagcc
    38161 ctgaccaggt ggggaatgtc tgagtgcgcg tgcgcggtcc gacattccca catcctgtcc
    38221 cgtttttcag gccattatag cctgttattt ttgtgcatca tagtcttatg cagacggcag
    38281 aggcagggca cagccctgcc aggagtcagt ttttgatgat aaaatcagac ttcaacatga
    38341 aacggagaga aagtgccaat ggcttctaaa aaattttact cagatgatga catccagctg
    38401 gcaaaagctg ctttatcaga attgccggac ctcaccgctc aacggaaaac attgcgtgat
    38461 tttcttgatg ccattcgtga tgacatcatt gtcctggtaa ggacaaaggg ttatacactg
    38521 gcagatgttc gtgacacgct caaaaatgca gggtatgaag taggagagaa agcacttcgt
    38581 gacattattc gggaagcaga atcgaaaaaa ccatcccgcc gttcttcctc taaaacagca
    38641 cctaaaaaga cagattcagg cagaaaagat ggcatcgata tgaacaacaa ttgatgtgac
    38701 aatgtcacat cgtgttctga gattatgatg attacagaaa aaaaagcccc ggcataaata
    38761 ccagggcttt tctgtttaat cagactcgtg gcggcaatgc atctggataa tgccgtgcaa
    38821 taatatcatt cagtttttca acaaggtcag aacgcttaaa gacaatgtgt acggtccctt
    38881 tctggtagcc acgcaccgta aaatattccc cttctaacag cttaccgaca ttgtcgccgt
    38941 tcccgagaaa ttcaccatac tgcgccccga tgctgttcct gaagtcagga acgttgcgcc
    39001 cctccagcag ataaaacgga cgtaccaggt catccagtgc ctcccgtccg ctgcagtcca
    39061 gcgtcacacg cccgttactc cagcggtaca gcaggttttc aataatgatt ttcttaccca
    39121 gacggcaggg attattggtt ctgtaatccc atgacaattt ccggtagaca tcaatcagcc
    39181 cctgttcaaa cgtctgcatc tttctggcat taagatggcg gaatgttgcc agtacattgt
    39241 ccagggtgat ttcaggacag gtgtcgctca tcagctggct gttccactca tcacgctggc
    39301 atgaactcat gaaggtgtac atgcccgtat ctgtcatcag gcggtcccag attttccggt
    39361 cggcaagacg ggtgatattc cttttcgctt cttcctgctt atccatgcga cgaacggaat
    39421 cagtcacgca gtcttcaaac ccgtaaagaa aaatatcacc tgacgcatcg agcagaatct
    39481 tgcgcgctgt cgccaggcat tccatcgcct gcatgaacag tgcaatacct tcgttacgct
    39541 gcgcgatgat gcggtcgata gccacagacg gaataacatc tgtcgtgccg gcagtattca
    39601 ggtcagcagt gggtaaagtc tgattcatca cattgtctcc tcatcgtcac gactgccggg
    39661 tcagccaggt catttcccgg aggagcgaag ctccttctgc cgtccgggtc ttcggggatt
    39721 tccccgcagt ggacctttcc gacgggggac gctcaccacg tcacggggca gttgtccggg
    39781 ggcagacgca aaagtttttg acgcctgcgg caaaaagttt tgtggctggt acatctctcc
    39841 cctggacgac tgaaacggga tgtggtcatg tatgtacccc cggaaagtcc gtctgcggag
    39901 aacaggccgc agtgcggcgg cagacaggca gagacagctg cgccatcagg cgcagtggcc
    39961 ttacacaccg taaggtgtgc agggagcgcc gcatacggcg cgactgaaga aaaacgacgg
    40021 cacaacaatg gcatgtggtg gtcactggca caccactccc gcacaggtga aaacagagaa
    40081 aaccgcacga catttacaag caaaaaaaag tgaaatttct tcattttttt acacttattt
    40141 atcgcgctga tttggcaatt gtgatgataa aataaagtga agttttttca ctttttcaaa
    40201 ggtgttttat gaccggctgg gaactgcgaa tctggcgaaa aagcatgctc tggtcacgcg
    40261 aaaaagcggc cagagaattt ggcgtgacgc agagaacatg gcacgcctgg gaaaatgcgg
    40321 aacaggtgga cgtcaccgtc tggaggacca cccaggcact ttcagtccgt gacctgctgc
    40381 ctcacatgca ggggatgcgt aaggcagata tcatcaggcg actggaaaat gaactggggg
    40441 aaacggcagg gaatgtgtga gcgtgattca cagcctggct gaatgccagg ctactgctca
    40501 tcaggaaaca gctttttcag ttcttcaccg gaaagtcccg tgacctcctg aatccgttcc
    40561 ggcggccagc ctatatcccg aagccggcag gctatttccc gcgccacttc aagagcccct
    40621 tgtttaaaac cctcgtcgaa acctttctgt ataagacgtt ctgcaatcgt catgctgttt
    40681 gctctactcc tgctgacaaa aaagcccggc attttgccgg gctttgtggt tctcatcagt
    40741 gagtgatttt cttcagttcg tcatcggaaa gtccggtagc ctgcttaatc tgctccggcg
    40801 tcatccccat tttctgaagc tggcacgcaa tagccagggc tttttcaagc gctccttcca
    40861 tgcgtccttc catgcgtcct tccatgcgtc cttccatcct gccttcctgg cgaccttttt
    40921 gctcaagacg ttctgcgata gtcatcagtt tatcctcatg ctgcggcaga cgctcagcca
    40981 gcgcccgcat aaattccggt gatacagagt cccctgcgtt aacgatgtaa tgaatcagaa
    41041 cacgcacctg ctcgtctgaa atttccacca tgacctgcgg gagtttgtcc agcagctcca
    41101 tcatgtcacg atggcggata tgcttcatca gcagcgtcag tgcggccatc cggcgatggt
    41161 tcatgatttc attgtcatca acgacagtga tatcaaccag cggaaacggc tttgtgtata
    41221 ttttagccgc aagcgccgga ttctcaaaac agtccagcca gttcatgctg tacgggtacg
    41281 ggctgcgctc accgtgatag aacagcaccg gaatgaccag tggcaacgtt ttgtgctgct
    41341 ccagatgtct ctgcatcgcc gccacactgt aacgtaacat gcggaaaggc atgtacagat
    41401 tttcggtgct ctggtgctcg atgagcgtat aaacatagcc atcttcaccc ccggtggttt
    41461 tcacgctcca gaggatatcg ctggcgtact gatgcaggtc cggctcaaca aaggtggcgg
    41521 gttccagctt cagcgtggac aggtcgcaca actgccggta ctccgccgga agatgcagtt
    41581 ccagaaaatc tctggcgacg tcggggttcg ccaggaacga ccggaacgcg gcatcatgcg
    41641 gcgtgggcgt tgtggtgttt ttcttcttac tcacggttcc tccatcaaat cagcagcatc
    41701 gcagcaatca tcgtccaaat ccccacaacc aagaggacag caattgcgat caaagccgtg
    41761 acaacaatag ccacgttctg cgtcacggcg tatttcttct gcaatctctt cagcggaaag
    41821 gtaatcaaac tcctcacggg ccagttccag gaccagcaga tgttgttctg tcggcaatgt
    41881 agacacaaaa ttatcaatag cagcactcaa acgtcgctga tatagctcat aaacaagacc
    41941 acaacggtgc attagttctt cggataatag ttccagtttt gagcgaacgc tgtcagcatt
    42001 gaaagtgact tcatcagatg acatctgaaa atctttgtca gtgtcaaaac aagacttcat
    42061 ttttatctcc tgcccgtccg ggccctgaat gaaagaaaac ctgtttccct tctctgtttt
    42121 tttatggaac aggaggaatc cggtaccatg cgacatagac gtcatgatgt ttttcatctt
    42181 gctcacggtt cctcctggta aagtacctga acatatcctg ccacctgcac agacagacgg
    42241 cagggggtaa tcagtggcag acccgttcaa tttcaagcgc acggagcagg tgcccccact
    42301 cgctgacttt catggcgggg atacgcaccc gcttccgctc actgcagcgt ctggcgatat
    42361 gaccgcccat cgccaggacg tccgggttaa tctcatccgg tgccagttcc tggcgtctga
    42421 agcgggtatg gcggtgcagg ggtttgttct cccgcaggca ctcttcacgc agacgcttta
    42481 catattcagc cgttgcaaag ataattgatg gtctcatggc ataatctcca tagttcgtga
    42541 atgagatgcc ggacttccgt acggggaagc gccggcagag aaaggcaggg tttatcgccc
    42601 tgcttttttt cattccggac agcgacgcag tgccagcgcg agcgctttca tcttccagcc
    42661 ttccggcgtg cggtacatca ccttccagta gccgtcgatg aacacataat ggaagcgcac
    42721 ctcttctgtg ctttcagtgg caatagcctc cctgaaagag cggtactcac gcgcttcact
    42781 gtccatccgg aaaccactgt cacgtccgta ataagccgtc acaccgtcca ccgggttctg
    42841 aagcgtatgc ccggcgggtt tgtcgcaacg gggaccgagg caacggatat ctccgtgttc
    42901 aaccagggct tcggccagct gaccggtggc atagtgggta tgcaggatgc gaccgttcca
    42961 ggtcggctca ccgtgcaggt ggcagtaaac ggtacggaac accccgtcgc ggcaaaaaac
    43021 ggaaatcgtg ctgctgactg tcatgaaaac gtctccttct gctgaatcag gtcgccgggc
    43081 cgaccggttg ctcccccgga ggggcgaaag ccccttctgc cgtccacttc ttcggggttt
    43141 ctccccgcag tggacctttc cgatggggca gcgacagcca cggcacgggg cagttgtcca
    43201 ggggcagacg caaaagtttt tgacgcttgc ggcaaaaagt tttgtggctg gtacatctcc
    43261 ccctggacgg ctgacacgtg ttgtggccgc ttcagccccc ggaaagtcca cagcgggaaa
    43321 caggccgcag gggtggcggc ggaagggcag acaactgcgc catcaggcgc agtttcctta
    43381 cacaccgtca ggtgtgcagg gagcgcaggt cacggcgcga cagaggcggc gcgaccggaa
    43441 cggtcgctgc aagccaggtt tttcagacat cggaccgtag cggcagcccg aaaccgcgcc
    43501 agcgggttcg gcggagactg gcgggtgcgc cagcagctgc ctgtcggagt cgggcctgac
    43561 gaccggatgc cgccaggcag ccgggagccg aaggtgtccc cgcctccgga aatcagaaca
    43621 acagaaacga cataacagaa aaatgcctca caccatgacg ggcaggaagg gcagcgccgc
    43681 aggcgcggcc ctcctgcatt tcaggcaggc gtaagcctgc cagggttttc cgggggcctg
    43741 gcaggccata cccccgccgg agcgggggag taaatcaccg acggaacaca tgaccgtcaa
    43801 taaaggtgaa ggagtccagg aataaatccc gcgcatacgc ctcatagtca aaatagagcg
    43861 ccaccgtctc cggcacgtca gccagcaggc cggtatcact ggcgaactca accgcaaaag
    43921 cctcctcact gtcagcctcg ccccaccagg catcgcggaa ggtgtcaaaa tccgtctcac
    43981 cggtatcctc cacccagaga cgataagcct cttcgcagcc ttcatcccgt gcctggcgga
    44041 agccttcaac ccaggcccag ttgatatggc attcagaggc catattcccc gggaatccct
    44101 cataatcctg aaacatcagt tcaggatcgg cttcatcctg gtgaagagca cggcaggcgg
    44161 cgaaaaagtc gcgctcatca tcaaacgtgg tcaggtcaaa ccagcgtccg gcgatgcttc
    44221 cacagttgta tttgtgccag gttccaacgt atacagcagg tgcaacaaca gacattcccg
    44281 aatcctccat agtcacatgt tctgcggcac cgggcggtgc cgtgcctcca taaaagccgg
    44341 gcgataaggc ggggatgcac agggcgcaag ggaagccgca gcctgacgca cacggctggc
    44401 gggagttgga gggtgagacc gcgaaatctg agcgggtgaa cgaccggaaa atcacggcag
    44461 acgggaagga gggaccgggt ggccgcagcg gcgaagcgta cccttgcatc cctgccagcc
    44521 cggctaagat ggaggcacgg cacagcccgc acacgacgct gaggccacag agctgaaaac
    44581 ccacaaggcc atgccccgca tggccgggta aaagcggagc cgccggcgac ttccttattc
    44641 cggcgagtgc agaggacacg ccgcatgacc tccagaccac ccctgatatg aaacgatcat
    44701 gatcggcgtg tccctgcgta aaatatacat gtctggttca ccggagaata tctgtgatat
    44761 ccacaagttt tctgtcgcac agccagtcct gcgcttcacg caccacgcta ctgtcgggtt
    44821 tcaccgcttt cagcgatgat gccagtcgct tgatcaacat aaccagttcc cgtgtctgta
    44881 tgtccggcat tgtctccggc ggagcagagg gactctcatt tccgacgccc atcccatgcg
    44941 cttccagtgt ggcacggaca aaccaggcgg catcgaccag ttgctggcga atccaggtca
    45001 gatcggcatt tctgtcacgg gctgccattc gctcacccga cagcgccttg ctgatatggc
    45061 tgcgcaccag attatccagg agttcctgcg cttcttccgg tgaaaagcca cctggtatct
    45121 ctgtgcgcaa cagtcctttc ctgtacagta gctgcagcag atcaatctgc gaccgcactg
    45181 caactatcag tacataacgg acaatattcg cactggcggt ggtttctgta tcattcagca
    45241 gttcctccgg atcattacca ccggtattgc agagcaacgg caacagaggt cgcatccaca
    45301 cgggaacctg atattgttcg tctgatttca tcatattgcg ttctccagct tgttcgggat
    45361 catccactgc tcaagtgcac gtacctgtac cggtgcacca cgaccggcct caccagcttc
    45421 aaaaatcagc cgccagagtg gttcctcctg ccagaaaaac cggtcaaatg atgacagtga
    45481 tggaaaacgc ccgcaccacc cccagaaaat cgtttccaga tcatgctcct caacaacacc
    45541 agaccagcgg gacaaccagc ttccgaccgt ggagggagta tgaaacgcaa ggtcaatctg
    45601 cgcctgagca aaacatgtct ggtattccag ttcccgtcgt tctgcctcac gttcccgctg
    45661 gcggctgtat ttctgctctg tcagcgtggt tttctgacga cgccgctcac cttcacgaaa
    45721 aggaacggac gggaacagac gatgccccgc atcggatggc aggggaagaa cacagacttc
    45781 accaaaactt tcaatcagtt tatccacggc agccagccac aggagacggt tgccatgaaa
    45841 ttcctccttc gtcagcgcag gagcaaaaaa gcggagatcg gtcagcgaaa tccggcgact
    45901 gccattcagg cagcgaaaga aagcgctggc ataaggccat gctgaaagtg tgcgtccctg
    45961 acgacgacgt tgttcggtga cagccacagc ctgcatggcg gcctgttgct ctgcgctgag
    46021 ggggatcagt gcccgtgaac gtgcgctcat gcctgacctc cttctgccat caggatatgg
    46081 atgatatcgt taacactgta cccctgcgca tcaagtgtcg cggcaagcgc cagcgtgtga
    46141 ctgatagcga caggcgtgaa gatatgttgc acctgaacga cggcgaaagg ctgtccacca
    46201 caattcacca gaaccagcat ccagagcaga gagacgtctc ccggcgaaca taatgccagg
    46261 tggaaacgtt cgtgggctgg cgtgaaacga acctggacgg ggaaaaagcc cccgaactca
    46321 ctgccatact cgccattcat catccagtta gccggtgcgt ccacttcacg catcacctcg
    46381 tgagtgagat tacggcgcat atcgcttccc gcagcacgga tatcttcata ttcctgtgcg
    46441 ttcatggact gcagggcatt cagggtcagt tcagttttca tcacggtatc tcccttgctg
    46501 ccgccccgtc tccggcgggg cggtgtggtt gttcatcagg cggcatcagc caggttatcg
    46561 gtgcgttcag tggcatctgt ctgtggatgt ggtgcacaca tccagccagg cacccagcgg
    46621 ttgtctttca tatggtgctc tgcatgttca gccgcatcac ctttcttcat cttctccgcg
    46681 tcccgtgcgg caccggacag tcctgcctca ttcagggctg cgataatctg cggctttttc
    46741 aggtgtccga agaagtttgc ttttgtcggc tgccaccagt cgcgcatgtg aaagccgatg
    46801 gcgctttcca gcgaatccag cggactgcgt gacgtgtgac cacactcacg ggtctggaca
    46861 ccgttaaggc tgcatgcggt gcagaaactg agcagggata acagcacctc ctgactgagt
    46921 gacaggaatg tcgtcatgtc ccgtgaccat ccctccggta acagggcagc aagacgggct
    46981 ttttctgcca tcagcgccat gaatgcggca ccttccttcc ccgatggcgc atcgctggtc
    47041 agcgaataat gttcacattc caggctgatt tgtgctggtt tactgtacgc cccgctgcca
    47101 aacacattca ggcagagcgt ccatgccagc agtgccagag atttgtccgg ctgctgcatg
    47161 agtgccgcct ggactgccag cgtgcgttcg gaagacattt tcgtcagcag tggcagactg
    47221 atttcctcca ctgacgcttt ctcctgcatc tgttccgtgc ggtcagcgtc gtcagccacg
    47281 tcatcctcac tgcgcaactg cacaccacgc tggacacaca cgttgccata acgccaggac
    47341 accaccacac cgcttccggc acgcatctcc ggcgtccacg ctctgacctt cgccatgcag
    47401 cgcatcagct tcatttctgc ttccagcagg tcggattcct cacactgatt ttccagcgcg
    47461 tcgtaacgcg tcatcagttc gttcaggcgt tcttcttccg cctccgtcag caccgcttcc
    47521 ggctccggca gactgcggta tgttccggca tcctcacggc actcaccgac aggctccatg
    47581 cgtccggcgc accattccca gccttcggct tcccgaaggt gttcagcgac agcctggagt
    47641 ttttccagca gggcagcatc gagcgcaaca cagtccacat agccaccctc gtcgtcgctg
    47701 aacaaatcgg tgcgcagttc atctggcgag aaggcatcag cccccacaaa gcggaattta
    47761 gtgttccccg ccaccgccac ttcactttcg gtcaccagac gacgaatggt ctgtacatcc
    47821 ggttttccgc cccatcccga ctggcaggcg gcttcaaaca cctgcacctg acgcgcggtg
    47881 tcgttctcca gcgccagcgc ctgacagtgc tctgtggtga tgcggtcttc tgccagcgca
    47941 tcgaggatga caggcgcaag gtctgccagt ttcagcattc gctgaacgtg gcggggcgaa
    48001 tagcccagca aatcaccgat ttgtgcaggc gttttgcctt cctgcgccat cgcacggaat
    48061 ccggcaatct gttcggcagg gtgcatatcc cgacgatgac cgttttcagt cattgatgcg
    48121 gcagtcgcca gctcctgcgg aataaccttc acgcgtacag gccagtcagc cggaatgatg
    48181 ccacgctctg ccagcatgtt gagtgcagcc agtcggcgac cacctgcggc gacaccgtaa
    48241 cggtcaccag gcagggtatg cacgaccaga ttctgcagca gtccgacgcc cttaatggac
    48301 tccgccagtt cgctgacgga ctccgcagaa tacggcaccg tgcgcacatt cagcggtgat
    48361 ttaatcagcg aggccagcgg cacgctcact tcctccgtct gcgccagcag ggcagacagg
    48421 acagtttcct gcgtttttac agatttacgg cttgctttgc gggtggtttc tggttcacac
    48481 ttcgttacag gcataatcat ccccttataa ttgcttatgt cctgccggac gttcgcgcgt
    48541 ccggcacctc tcttacttaa gaatcccgaa atccctgatg tactggttca tccagtaccc
    48601 ggcaccgtcc tcaaaattcc aggagcgcca gatcaacgtg ccatcctgac ggacaaccag
    48661 gcgaaaatgc gttccctgat catcctcgat aaagacgttc cggtactgtg cggcaacggc
    48721 ttccgcctgt tcgcgggtaa aggagccgtc cggcagtccc tgaagtattc tgaaatattc
    48781 gctcatcctc atatctccgg tgatgccccg cacagggcgg ggcggttgtc acagtcagcc
    48841 cgatcagaac gggatgtcgt ctgaaaaccc gtaatcctcc ccctccggcg gttgcggctg
    48901 aggctctggc tgcgcggcct tgcgtccacg gcctttcgtt ttcgcgccac cttttttcgt
    48961 cgcgtcagca ctctgtggct gcccattctg ctgaggcttc ggttgcgcct gagcgttctg
    49021 ctgtggtgca cgtcccagca tctgcacggt gcccgtggtc ttaacaagaa tttcagtgac
    49081 gtaacgggtg ataccgttat cttcccagct acgggtgcga agctgacctt cgatgtagac
    49141 ctgcgcaccc ttgcgcagat attcacctgc cacttccgcg agcttgccga acagcaccac
    49201 gcggtgccat tccgtctgct cccgcatctc ccccgtctgt ttgtcacgcc agctttctga
    49261 tgtggccacc tgcaggtttg ccactgcgcc cccgttgggg atgtaacgga cttccggatc
    49321 attgcccaga cgcccgacga ggatgacctt gttgatacca cgtgcactca taagaacctc
    49381 ctgactgaaa gatgtgagat aaaccggata ccggcgacgg gacgttcatg tgccccttca
    49441 gaggagcgaa agctcctcct gccgtccact tcacaggggt ttctccccgc agtggacctt
    49501 tccgatgggg gcccgtttac cgcctcacgg gacagctgtc cgggggcgga cgcaaaattt
    49561 ttagccgggt tcatggctaa aaattttgtg gccggtgcat ctcaccctgg acagatggaa
    49621 cgggaggggg ttacgtctgc cccccggaaa gtccacagcg ggaaacaggc cgcaggggtg
    49681 gcggcaggag ggaagactgc tgcgccatca ggcgcagttt cctcacacac cgtcaggtgt
    49741 gcagggagcg caggtcacgg cgcgacaggg gcggcgcgac cggaacggtc gctgcaagcc
//
LOCUS       DAZFQD010000015        49658 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.015, whole genome shotgun sequence.
ACCESSION   DAZFQD010000015 DAZFQD010000000
VERSION     DAZFQD010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 49658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49658
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.015"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(62..1306)
                     /gene="mtr"
                     /locus_tag="ACHKTW_000997"
     CDS             complement(62..1306)
                     /gene="mtr"
                     /locus_tag="ACHKTW_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HFO6462064.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSALGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMIALIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(1460..3349)
                     /gene="deaD"
                     /locus_tag="ACHKTW_000998"
     CDS             complement(1460..3349)
                     /gene="deaD"
                     /locus_tag="ACHKTW_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HFO6462065.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(3342..3422)
                     /gene="yrbN"
                     /locus_tag="ACHKTW_000999"
     CDS             complement(3342..3422)
                     /gene="yrbN"
                     /locus_tag="ACHKTW_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HFO6462066.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(3529..4413)
                     /gene="nlpI"
                     /locus_tag="ACHKTW_001000"
     CDS             complement(3529..4413)
                     /gene="nlpI"
                     /locus_tag="ACHKTW_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HFO6462067.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(4522..6657)
                     /gene="pnp"
                     /locus_tag="ACHKTW_001001"
     CDS             complement(4522..6657)
                     /gene="pnp"
                     /locus_tag="ACHKTW_001001"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFO6462068.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSRDGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(6904..7173)
                     /gene="rpsO"
                     /locus_tag="ACHKTW_001002"
     CDS             complement(6904..7173)
                     /gene="rpsO"
                     /locus_tag="ACHKTW_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFO6462069.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(7322..8266)
                     /gene="truB"
                     /locus_tag="ACHKTW_001003"
     CDS             complement(7322..8266)
                     /gene="truB"
                     /locus_tag="ACHKTW_001003"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFO6462070.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERMVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(8266..8667)
                     /gene="rbfA"
                     /locus_tag="ACHKTW_001004"
     CDS             complement(8266..8667)
                     /gene="rbfA"
                     /locus_tag="ACHKTW_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFO6462071.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(8831..11503)
                     /gene="infB"
                     /locus_tag="ACHKTW_001005"
     CDS             complement(8831..11503)
                     /gene="infB"
                     /locus_tag="ACHKTW_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFO6462072.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQAQREAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSN
                     QQDDMTKNAQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDN
                     AEPTEDSSDYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADRE
                     EARAAVRGGKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIK
                     AMMKLGAMATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAP
                     VVTIMGHVDHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAA
                     FTSMRARGAQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPD
                     RVKNELSQYGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMAS
                     GAVIESFLDKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSI
                     PVEILGLSGVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMT
                     EGEVHEVNIVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASN
                     AILVGFNVRADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLA
                     EVRDVFKSPKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVR
                     NGMECGIGVKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(11528..13015)
                     /gene="nusA"
                     /locus_tag="ACHKTW_001006"
     CDS             complement(11528..13015)
                     /gene="nusA"
                     /locus_tag="ACHKTW_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFO6462073.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGIDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(13043..13495)
                     /gene="rimP"
                     /locus_tag="ACHKTW_001007"
     CDS             complement(13043..13495)
                     /gene="rimP"
                     /locus_tag="ACHKTW_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFO6462074.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(13702..13778)
                     /locus_tag="ACHKTW_001008"
     tRNA            complement(13702..13778)
                     /locus_tag="ACHKTW_001008"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13742..13744),aa:Met,seq:cat)
     gene            14126..15469
                     /gene="argG"
                     /locus_tag="ACHKTW_001009"
     CDS             14126..15469
                     /gene="argG"
                     /locus_tag="ACHKTW_001009"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HFO6462075.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSATSGVPQVENMENKGQ"
     gene            complement(15477..17101)
                     /gene="yhbX"
                     /locus_tag="ACHKTW_001010"
                     /pseudo
     CDS             complement(15477..17101)
                     /gene="yhbX"
                     /locus_tag="ACHKTW_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417641.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
     gene            complement(17560..17646)
                     /locus_tag="ACHKTW_001011"
     tRNA            complement(17560..17646)
                     /locus_tag="ACHKTW_001011"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17610..17612),aa:Leu,seq:gag)
     gene            complement(17661..17993)
                     /gene="secG"
                     /locus_tag="ACHKTW_001012"
     CDS             complement(17661..17993)
                     /gene="secG"
                     /locus_tag="ACHKTW_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFO6462076.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNKGSEWENLSAPVKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(18221..19558)
                     /gene="glmM"
                     /locus_tag="ACHKTW_001013"
     CDS             complement(18221..19558)
                     /gene="glmM"
                     /locus_tag="ACHKTW_001013"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFO6462077.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGNRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(19551..20399)
                     /gene="folP"
                     /locus_tag="ACHKTW_001014"
     CDS             complement(19551..20399)
                     /gene="folP"
                     /locus_tag="ACHKTW_001014"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HFO6462078.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(20489..22423)
                     /gene="ftsH"
                     /locus_tag="ACHKTW_001015"
     CDS             complement(20489..22423)
                     /gene="ftsH"
                     /locus_tag="ACHKTW_001015"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFO6462079.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(22523..23152)
                     /gene="rlmE"
                     /locus_tag="ACHKTW_001016"
     CDS             complement(22523..23152)
                     /gene="rlmE"
                     /locus_tag="ACHKTW_001016"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016436 - rRNA (uridine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HFO6462080.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            23278..23571
                     /gene="yhbY"
                     /locus_tag="ACHKTW_001017"
     CDS             23278..23571
                     /gene="yhbY"
                     /locus_tag="ACHKTW_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFO6462081.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(23727..24203)
                     /gene="greA"
                     /locus_tag="ACHKTW_001018"
     CDS             complement(23727..24203)
                     /gene="greA"
                     /locus_tag="ACHKTW_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFO6462082.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            24451..25884
                     /gene="dacB"
                     /locus_tag="ACHKTW_001019"
     CDS             24451..25884
                     /gene="dacB"
                     /locus_tag="ACHKTW_001019"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HFO6462083.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYVTQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPGDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     EPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(25924..27096)
                     /gene="cgtA"
                     /locus_tag="ACHKTW_001020"
     CDS             complement(25924..27096)
                     /gene="cgtA"
                     /locus_tag="ACHKTW_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HFO6462084.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(27112..28077)
                     /gene="yhbE"
                     /locus_tag="ACHKTW_001021"
     CDS             complement(27112..28077)
                     /gene="yhbE"
                     /locus_tag="ACHKTW_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFO6462085.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(28204..28461)
                     /gene="rpmA"
                     /locus_tag="ACHKTW_001022"
     CDS             complement(28204..28461)
                     /gene="rpmA"
                     /locus_tag="ACHKTW_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFO6462086.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(28482..28793)
                     /gene="rplU"
                     /locus_tag="ACHKTW_001023"
     CDS             complement(28482..28793)
                     /gene="rplU"
                     /locus_tag="ACHKTW_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFO6462087.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            29052..30023
                     /gene="ispB"
                     /locus_tag="ACHKTW_001024"
     CDS             29052..30023
                     /gene="ispB"
                     /locus_tag="ACHKTW_001024"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HFO6462088.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            30250..30528
                     /gene="sfsB"
                     /locus_tag="ACHKTW_001025"
     CDS             30250..30528
                     /gene="sfsB"
                     /locus_tag="ACHKTW_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HFO6462089.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(30576..31835)
                     /gene="murA"
                     /locus_tag="ACHKTW_001026"
     CDS             complement(30576..31835)
                     /gene="murA"
                     /locus_tag="ACHKTW_001026"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFO6462090.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(31890..32144)
                     /gene="ibaG"
                     /locus_tag="ACHKTW_001027"
     CDS             complement(31890..32144)
                     /gene="ibaG"
                     /locus_tag="ACHKTW_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HFO6462091.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(32304..32597)
                     /gene="mlaB"
                     /locus_tag="ACHKTW_001028"
     CDS             complement(32304..32597)
                     /gene="mlaB"
                     /locus_tag="ACHKTW_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HFO6462092.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(32597..33232)
                     /gene="mlaC"
                     /locus_tag="ACHKTW_001029"
     CDS             complement(32597..33232)
                     /gene="mlaC"
                     /locus_tag="ACHKTW_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HFO6462093.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(33251..33802)
                     /gene="mlaD"
                     /locus_tag="ACHKTW_001030"
     CDS             complement(33251..33802)
                     /gene="mlaD"
                     /locus_tag="ACHKTW_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HFO6462094.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTEPTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(33807..34589)
                     /gene="mlaE"
                     /locus_tag="ACHKTW_001031"
     CDS             complement(33807..34589)
                     /gene="mlaE"
                     /locus_tag="ACHKTW_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HFO6462095.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(34597..35406)
                     /gene="mlaF"
                     /locus_tag="ACHKTW_001032"
     CDS             complement(34597..35406)
                     /gene="mlaF"
                     /locus_tag="ACHKTW_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HFO6462096.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQANPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            35616..36593
                     /gene="yrbG"
                     /locus_tag="ACHKTW_001033"
     CDS             35616..36593
                     /gene="yrbG"
                     /locus_tag="ACHKTW_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HFO6462097.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQAERQGTDSLTR
                     EQLAELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMGLTAIAI
                     GTSLPELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDY
                     SVMLLVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            36607..37593
                     /gene="kdsD"
                     /locus_tag="ACHKTW_001034"
     CDS             36607..37593
                     /gene="kdsD"
                     /locus_tag="ACHKTW_001034"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HFO6462098.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPGEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            37614..38180
                     /gene="kdsC"
                     /locus_tag="ACHKTW_001035"
     CDS             37614..38180
                     /gene="kdsC"
                     /locus_tag="ACHKTW_001035"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HFO6462099.1"
                     /translation="MSKAGASLATCYGPVSADVIAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLHAQGKLDEAKGQSI"
     gene            38177..38752
                     /gene="lptC"
                     /locus_tag="ACHKTW_001036"
     CDS             38177..38752
                     /gene="lptC"
                     /locus_tag="ACHKTW_001036"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HFO6462100.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            38721..39278
                     /gene="lptA"
                     /locus_tag="ACHKTW_001037"
     CDS             38721..39278
                     /gene="lptA"
                     /locus_tag="ACHKTW_001037"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HFO6462101.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            39285..40010
                     /gene="lptB"
                     /locus_tag="ACHKTW_001038"
     CDS             39285..40010
                     /gene="lptB"
                     /locus_tag="ACHKTW_001038"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HFO6462102.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            40058..41491
                     /gene="rpoN"
                     /locus_tag="ACHKTW_001039"
     CDS             40058..41491
                     /gene="rpoN"
                     /locus_tag="ACHKTW_001039"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HFO6462103.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            41514..41801
                     /gene="hpf"
                     /locus_tag="ACHKTW_001040"
     CDS             41514..41801
                     /gene="hpf"
                     /locus_tag="ACHKTW_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HFO6462104.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            41919..42410
                     /gene="ptsN"
                     /locus_tag="ACHKTW_001041"
     CDS             41919..42410
                     /gene="ptsN"
                     /locus_tag="ACHKTW_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HFO6462105.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            42456..43310
                     /gene="rapZ"
                     /locus_tag="ACHKTW_001042"
     CDS             42456..43310
                     /gene="rapZ"
                     /locus_tag="ACHKTW_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFO6462106.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            43307..43579
                     /gene="npr"
                     /locus_tag="ACHKTW_001043"
     CDS             43307..43579
                     /gene="npr"
                     /locus_tag="ACHKTW_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HFO6462107.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            43792..44424
                     /gene="yrbL"
                     /locus_tag="ACHKTW_001044"
     CDS             43792..44424
                     /gene="yrbL"
                     /locus_tag="ACHKTW_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HFO6462108.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSGEA"
     gene            complement(44421..45149)
                     /gene="mtgA"
                     /locus_tag="ACHKTW_001045"
     CDS             complement(44421..45149)
                     /gene="mtgA"
                     /locus_tag="ACHKTW_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HFO6462109.1"
                     /translation="MSKSRLTVFSFVRRFLLRLMVVLAVFWGGGIALFSVAPVPFSAV
                     MVERQVSAWLHGNFRYVAHSDWVSMDQISPWMGLAVIAAEDQKFSEHWGFDVASIEKA
                     LAHNERNENRIRGASTISQQTAKNLFLWDGRSWVRKGLEAGLTLGIETVWSKKRILTV
                     YLNIAEFGDGVFGVEAAAQRYFHKPASKLTRSEAALLAAVLPNPLRFKVSAPSGYVRS
                     RQAWILRQMYQLGGEPFMQQHQLD"
     gene            complement(45146..45799)
                     /gene="elbB"
                     /locus_tag="ACHKTW_001046"
     CDS             complement(45146..45799)
                     /gene="elbB"
                     /locus_tag="ACHKTW_001046"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HFO6462110.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFATLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(46029..48365)
                     /gene="arcB"
                     /locus_tag="ACHKTW_001047"
     CDS             complement(46029..48365)
                     /gene="arcB"
                     /locus_tag="ACHKTW_001047"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HFO6462111.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(48461..49390)
                     /gene="yhcC"
                     /locus_tag="ACHKTW_001048"
     CDS             complement(48461..49390)
                     /gene="yhcC"
                     /locus_tag="ACHKTW_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312117.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HFO6462112.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGQAECLQTLERVVETGVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALERPWIPPTE"
BASE COUNT        12218 a        13465 c        11983 g        11992 t
ORIGIN      
        1 gataagcgaa gcgcatcagg cattgtgtag gcagcagaaa tgtcggataa ggcaccgctg
       61 attactgata caccggcagt aaattaaagc tcgataaaat atgcaccagt gcgttgccga
      121 cgccaaacac cagaatcagc gcaatcatcg gcttgccacc ccagacgcgg aatttcgggc
      181 tgccaaagcg tttacgcgat gcgcgggcta acagcgccgg aacaattgcc gcccagatgg
      241 tagctgctaa accagcataa ccaatggcgt acaggaatcc gttcgggaac agtagaccac
      301 ccacaacagg cggggcaaag gtcagcaatg ccgttttcaa gcgccccagg gctgagtcat
      361 cgaaaccaaa cagatctgcc agatagtcaa acaaacccag cgttacgcca aggaacgaac
      421 tcgctaccgc aaagtttgag aacacgacca gcagaaggtc caggctacgg ctgttcagca
      481 cgccgcttaa tgcctgtacc agcacatcaa tattaccgcc cttctctgca ataccgataa
      541 actccggacg cgggatgtta cccatcgtcg ccagcaacca gatggtatac agcgccagtg
      601 ccatcagcgt accgtacacc aggcatttca cgatggtttt cggatctttg ccgtaatact
      661 tcatcaggct tggcacgtta ccgtgataac caaacgacgc caggcagaac ggcaatgtca
      721 tcagcagata cggcgcataa gatgcattgc tttctgcaac gttgaacaat gtcgcaggct
      781 gcacatgccc caacaggctg ccaaaggtga ggaagaaggt aatgactttc gcccccagca
      841 caatcgccgt catgcggctg acggccttag tactcaacca caccacaaac gctaccagca
      901 gtgcaaaacc aaaacccgcc gcccgtgccg ggacgtttag cgacatctct gcgaaggtgt
      961 gatgcagaat tgaaccactg gcagaaatat aggcataggt caggatatag agcacaaagg
     1021 caatggaaat accgttgacc acgttccagc ctttgcccag caaatctttg gtgatggtgt
     1081 caaaactcga accgattcgg taattcaggt tagcttccag aatcatcaag ccggaatgca
     1141 gcatacagaa ccaggtaaag atcagcgccg ccattgacca gaaaaaccac gccccggaca
     1201 tgaccactgg cagagaaaac atccctgcgc caataatggt gccgccgata atcaccacgc
     1261 cgccaagcag cgacggtgac gtttgggtgg tggttagtgt tgccatgagg gcttctctcc
     1321 agtgaaaaat agtgcgactg cgttgttatg cattgcactg taccagtaca cgagtacaaa
     1381 agacagaaaa aaagccccga tggtaaaaat cggggctgta tatattattt tacagattgt
     1441 gttcgctgtt cagcgatgat tacgcatcac caccgaaacg acgacgaccg gtagagtcat
     1501 cacgacgcgg agcgcggcct tcacgacgtt cgccgctaaa acgacgacca tcaccacggc
     1561 caccttcacg gcgttcaccg ctgaagttac gaccgccttc acgacgttcg ccaccgaaac
     1621 cacgaccacc gccacgacgc tcaccgccag tatgcggctg tgcatcgccc agtaactgca
     1681 tgttcatcgg cttgttgaga atgcgagtgc gcgtaaagtg ttgcagcact tcacccggca
     1741 tacctttcgg cagttcgatg gtggagtgag aagcaaacag cttgatgtta ccaatgtaac
     1801 ggctgctgat gtcgccttcg ttagcaatcg caccaacgat atgacgaact tcaacaccat
     1861 catcgcggcc cacttcaatg cggtacagct gcatatcgcc aacatcacga cgttcacgac
     1921 gcggacgatc ttcacggtca ccacgcgggc cacggtcgtt acgatcgcgc ggaccacggt
     1981 catcacggtc acggaattca cgtttcggac gcatcggcgc atctggcggt acgatcagag
     2041 tacgttcacc ctgtgccatt ttcagcagtg ccgcagccag agtttcgaga tccagctctt
     2101 caccttctgc agtcggctga attttgctca gcagtgcgcg gtattgatcc agatcgctgc
     2161 tttccagctg ctgctgtact ttagcggcga atttttccag acggcgtttg cccagcagtt
     2221 ctgcgttcgg cagttctact tccggaatag tcagcttcat agtacgttca atgttgcgca
     2281 gcagacgacg ctcgcggttc tcaacgaaca gcagcgcgcg gccagcacga cccgcacgac
     2341 cggtacgacc gatacggtga acgtaagact cagaatccat cgggatatcg tagttaacta
     2401 ccaggctgat acgctcaacg tccaggccac gggctgcaac gtcggtcgca atcaggatgt
     2461 ccagacgacc atctttcagg cgttccagtg tctgctcacg cagcgcctgg ttcatgtcac
     2521 cgttcagcgc ggcactgttg tagccgttac gctcaagagc ttcagccact tccagagtcg
     2581 catttttggt acgaacgaag ataatcgccg catcaaaatc ttccgcttcc aggaaacgta
     2641 ccagtgcttc gtttttacgc ataccccaga cagtccagta gctctggctg atgtcaggac
     2701 gggtagtcac gctggactga atgcgcactt cctgcggctc tttcataaag cggcgggtaa
     2761 tgcgacgaat cgcttccggc atggttgcag agaacagagc ggtctgatga ccttccggga
     2821 tctgcgccat aatggtttca acgtcttcga tgaagcccat gcgcagcatt tcgtcagctt
     2881 catccagaac cagaccgctc agtttagaga ggtccagagt gccacgtttc aggtggtcca
     2941 gcagacggcc cggagtaccg acaacgatct gcggcccctg acgcagggcg cgtaattgca
     3001 cgtcataacg ctggccgccg tatagagcaa ccacatttac gccgcgcatg tgtttagaga
     3061 aatccgtcat tgcttcagca acctgtaccg ccagttcgcg ggtcggtgcc agcaccagaa
     3121 tctgtggtgc tttcagctca ggatcaagat tctgcaacag aggtaaagag aatgctgcag
     3181 tttttccgct ccccgtctgg gccataccca gaacgtcgcg gccattcagc agatgtggaa
     3241 tacactctgc ctgaattgga gatggttttt cgtaacccag atcgttaagg gcttcaagga
     3301 taggagcctt caggcccaga tctgcaaaag tggtttcgaa ttcagccatg tagtacgtgt
     3361 gcctcaaaat taatggcggc cagtctacat aactcatcat gaaattgatc agcaattttc
     3421 attgaaaagt gtgaaccggc tcaaagtagg tgtattaacg aacaacaacg ccctcacccg
     3481 ttaaggtgat ggcaatcaaa aaagattacg ggctgatgtg tacgtcagct attgctggtc
     3541 cgattctgcc aggtcatctt ggtcctggcc caggagcgat aattccaaca atgcgtatcg
     3601 gtgctcaaca aagttatgaa cgttgttggc aaccgccagt ttgaacagtg ccgtggcgct
     3661 gtccaaatcc cccagactta ggtagtactt acctaaatag aagttggttt cactgagatg
     3721 ctcagcgagc gaggtgttat ccgttgcgtc cgccttgagc ctttccatta acgtttgttc
     3781 gctaatgttg cccaggtaga actcgacaat gttccatccc cactgttcct tatccgattt
     3841 ttcgaagtgc tgtttcaaca cttctttagc ctgcttctca tcgagcttct gctcggcgag
     3901 ataaagccac agactacgga aaggatcatt gggatcgtct tgataaaacg ccagcagatc
     3961 atcttgcgct aacttgtcac gaccgccgta atataatgcg atcccgcgat tcaagtgcgc
     4021 gtagttgtaa gttggatcaa gctcaagtac agaatcaaac gcttcatagg cagcatcaaa
     4081 attgcctgcc tgcgttaaat atatgcctaa gtaattgaat acttcaggca tatccggtcg
     4141 gattgccagc gcttgcgaaa aatcgttacg cgctaatgcc ctcagaccga gactatcata
     4201 caacactccg cgctcatata aaagctgtgc gcgttcgtca tcggttaaag cccgactggc
     4261 aaggatttgt tccatacgtg ccagaatcac ttcctgctgt aaagtcggtt gcaatggtac
     4321 cgcgaggact tcacttttac gccaggaagt attactgcat cctgcaagcg taagtgctgt
     4381 cgcaacgaaa caccagcgca aaaaaggctt catttcccac tcccgaagac cacggttgaa
     4441 tgaacgtcct gttcccggtt gctaacaagg cgtcctgccc ggttaaaagc cccccgccgc
     4501 agcggagggc aaatggcaac cttactcgcc ctgttcagca gccggagctt ccggtgctgc
     4561 agcaggttga gactgctcag tcgcttcttt aatgctcaga cggatacggc cctggcgatc
     4621 aacttccaga actttcaccg gtacttcctg acccatctgc aggtaatcgg tcactttctc
     4681 aacgcgtttg tcagcgattt gagagatgtg gaccagacct tctttaccgc cgccgatggc
     4741 aacaaatgcg ccaaagtcaa cgatacgggt cactttacca gtgtagacgc ggcccacttc
     4801 gatttctgca gtgatctctt cgatacgacg aatagcatgt ttcgctttct cgccgtcggt
     4861 cgctgcgatc ttcacagtac cgtcatcttc gatttcgatg gtagtgccag tttcttcggt
     4921 cagggcacgg ataacagagc cgcctttacc gataacgtct ttgatcttat ccgggttgat
     4981 cttgatggta tggatacgcg gtgcgaactc agagatatcg ccacgcggcg cgttgatcgc
     5041 ctgttccatt acgcccagga tatgcagacg cgcaccttta gcctggttca gcgcaacctg
     5101 catgatctct ttggtgatac cttcaatttt gatatccatc tgcagtgcag agataccgtc
     5161 gcgggaacct gcaactttga agtccatatc gcccaggtga tcttcgtcgc ccaaaatgtc
     5221 agacagtaca acgtagttgt cgccttcttt caccagaccc attgcgatac ccgcaacggc
     5281 agctttgatt ggcacacctg cgtccatcag cgccagagac gcgccgcaca cggaagccat
     5341 agaagaagaa ccgttggatt cggtgatttc agacactaca cgtacggtgt acgggaattt
     5401 gtccatatcc ggcatgactg ccagcacgcc gcgcttcgcc agacgaccgt gaccaatttc
     5461 acgacgcttc ggagaaccga ccatgccggt ttcgcctacg gagtacggag ggaagttgta
     5521 gtggaacagg aaggtatcgg tacgttcgcc catcagttca tcaagaacct gcgcgtcacg
     5581 agcggtaccc agcgttgcag tgaccagtgc ctgcgtttca ccgcgggtga acagcgcaga
     5641 accgtgagta cgcggcagca cgccagtacg cacatccaga ccacggatca tatctttttc
     5701 acgaccgtcg atacgcggtt cgcctgccag tacgcggcta cgaacaacgt ttttctcgat
     5761 agcgtgcaga atttcaccca gttcgttttc gtccagggtt tcgtcttcag caagcagcgt
     5821 cgcgatggtt tcagatttga tgacatcaac ctgcgcataa cgctcttgtt tgtcggtgat
     5881 gcggtaagca tcgctcaggc gagcttcagc cagtgcagca acgcgcgcgt tcagcgcttc
     5941 gtttaccggc tccggctgcc agtcccaacg tggtttaccg gcttctttca ccagttcatt
     6001 gatgttctga ataacaacct gctgttgttc atgaccgaac actactgcgc ccagcatctg
     6061 gtcttcgctc agcagttcag cttcagattc aaccatcaga acagcggctt cagtaccggc
     6121 aacaaccaga tccagtttgc tctctttcag ctcgtcctga gtcgggttca gtacgtactg
     6181 gtcattgatg taacctacgc gagcagcacc aatcggacca ttgaacggaa taccagacag
     6241 agacagtgct gcggaagcac caatcatcgc gacgatatcc gggttaactt gcgggttaac
     6301 agaaaccacg gtggcgataa cctgaacttc gttgacgaag ccttccggga acagcgggcg
     6361 aatcgggcgg tcaatcagac gcgcgatcag ggtttcgcct tcgcttgggc ggccttcacg
     6421 acggaagaag ctacccggga tacgaccagc agcgtaggta cgctcctgat agttaacggt
     6481 cagtgggaag aagtcctgac ctggtttggc ttttttctgg ccaacaacgg taacaaatac
     6541 cgcggtgtca tccatgctaa ccataacagc ggcagtagcc tgacgagcca tcatgccggt
     6601 ttccagagtc acggtgtgtt ggccgtactg gaatttacga acgatcggat taagcaatgt
     6661 aatatccttt ctctttctta gacagtacct tacggcactg gtgttaatac ccgatcttct
     6721 gcgcatcctc gcgactaatg acaaccctaa cccagctcta tgtgggtaaa gcctctcatt
     6781 agccgcgcga acctctgcaa cggaagatca ttcatagcaa caatacatta gtttccagtg
     6841 aattgctgcc gtcagcttga aaaaaggggc cactcaggcc cccttttctg aaactcgcaa
     6901 gaattagcga cgcagaccca ggcgctcgat gagctgggtg taacgtgcta cgtctttacg
     6961 tttcaggtag tcgagcagtt tacgacgctg agaaaccatg cgcagcagac cacgacggct
     7021 gtggtgatct tttttgtgct ctgcaaagtg gccctgcagg tggttgatct gtgcagtcag
     7081 cagtgctacc tgaacttcgg tagaaccggt gtcgtttgcg tcacgaccaa actcagaaac
     7141 gattttagct gttgcttcag tacttagaga cattttaaaa ctccaaagta tatagaatga
     7201 aaggacgccg atctctaatt cagcgatccc agtgtacgtt acgcaaagtt ttaaacaatt
     7261 tacgcgacgt taagcggcag tattctactc gtagcgacct gttatcgcaa gacggttaac
     7321 attacgccgg gtattcaacc accaggcgac gaggcgcaac gcggccttca tcgtcaattt
     7381 cgcccatacc gataaatttg ccgttctcac cttctgtaac gcgaaccagt ccttccagcg
     7441 gcgcaccgga tgtacgaact gggttaccat ttttgaagta aacagaagac gttaacggaa
     7501 gattcaccac cgggtagtcc gaagctggac tgtccattgg catcagtaat ggatcaagta
     7561 actccgcagc cggaatatcc tgctgttcgg cttgttcgac cagttcacgc agatgttcca
     7621 gggtcaccat ccgttcaacc ggatatttac ttaccgccag acgacgcagg taaataacat
     7681 gcgcgccaca accgagtttt tcacccaggt catcaatgat ggtgcggata taagtgcctt
     7741 ttgagcagtg aatttccagc tccagctcat tgccttcgtg gcgaataaac agcaattcat
     7801 aaacggtaat cgggcgcgct tcacgcggta cttcaatgcc ctgacgcgca tattcgtaca
     7861 gttttttacc ctgatatttc agtgctgaat acatcgaagg gatctgttcg atatcgccac
     7921 ggaaagtatc cagcgccgcc gccagttgct ctgcgctaaa ggttaccgga cgttcttcaa
     7981 cgatttgtcc gtcggcatca gaagtatcgg tacgctgtcc aagacgcgca atgacccgat
     8041 agcgtttgtc ggagtccagc agatactggg aaaacttcgt cgcttccccg aggcaaattg
     8101 gcaacatgcc agttgccagc gggtccagcg caccggtatg cccggcacgg ttggcgttat
     8161 aaatccgttt cactttttgc agcgcatcat tgctggacat accctgcggt ttatccagca
     8221 acaaaacgcc gttaatgtcg cgaccgcgac gacgaggacg actcattagt cctccttgct
     8281 gtcgtccggg ttaacacgac gttcttcgtc atgtttgacc acgctggtca ccaggtttga
     8341 catgcgcatc ccttcaacca gagagttgtc gtagaagaag gtcagttccg gcacgatacg
     8401 caggcgcatc gctttcccca gcaggctgcg gatgaaacca gaagcttctt gcaacgcttt
     8461 gatgcccgct ttaaccgcgt cttcatcttt gtcgttgagg aacgtcacat atactttggc
     8521 atacgccagg tcgcgagaca tttcgacacc ggaaacggtg gtcatcatgc ccaggcgagg
     8581 atctttaatt tcacgctgca ggatgagagc gatctctttt tgcatttcct gcgctacgcg
     8641 ctgcgggcga ccaaattctt tcgccataat aaattctcct gacaaaaaag gggctgttag
     8701 ccccttttta aaattaattt caggtggaag ggctgttcac gttggcctga taagacgcgc
     8761 cagcgtcaca tcaggcaacg gctgccggat gcggcgtaaa agccttatcc ggcctacaac
     8821 ccagacaacc ttaagcgatg gtacgttgga tttcgatgat ctcgaatact tcgatcacat
     8881 cgccagtgcg gacatcgttg tagttcttaa cgccgatacc acattccata ccgttacgga
     8941 cttcgttaac gtcatctttg aagcggcgca gggactccag ctcgccttcg tagataacca
     9001 cgttgtcacg cagaacgcgg atcgggttgt gacgtttaac cacaccttcg gtaaccatac
     9061 agcctgcgat ggcaccaaat ttcggcgatt tgaacacgtc acgaacttcc gccagaccga
     9121 taatctgctg tttcagttcc ggagacagca taccgctcat cgccgctttc acttcgtcaa
     9181 tcaggttata gatgacggag tagtaacgca gatccaggct ttccgcttca atcactttac
     9241 gtgcagaggc atcagcacgt acgttgaagc caaccaggat ggcgttggac gccgcagcca
     9301 gggtggcgtc ggtttcggtg ataccaccta cgccagaacc gatgatcttc actttaactt
     9361 cgtcagtaga cagtttcagc aaggagtcgg agatcgcttc cacagaaccc tgtacgtctg
     9421 ccttcaggac gatgttcact tcgtgaactt cgccttcggt catgttggcg aacatgttct
     9481 cgagtttaga tttctgctga cgcgccagtt taacttcgcg gaatttaccc tgacgataca
     9541 gtgcaacttc acgcgctttc ttctcgtcac gtacaacggt aacttcatca cccgcagccg
     9601 gtacgccgga caggccgagg atttccaccg gaatggacgg acccgcttcc agcacttcct
     9661 gacccagttc gttacgcatc gcacgaacac gaccgtattc gaagccacac agaacgatat
     9721 cgcccttgtg cagagtacct tcacgtacca gaacggtagc aaccggacca cgacctttat
     9781 cgaggaagga ttcgataacc gcaccgctcg ccataccttt acgtaccgct ttcagctcca
     9841 gaacttccgc ctgcagcagg atagcgtcca gcagttcatc gatgccggta cccgctttcg
     9901 cagatacgtg tacgaactgg ctttcaccgc cccactcttc cggcaggatg ccatactggg
     9961 agagttcgtt cttaacacga tccggatcgg cttctggttt atcaatcttg ttcactgcaa
    10021 ccaccaccgg tacctgcgcc gctttcgcgt gctggattgc ttcgatggtc tgcggcatca
    10081 cgccgtcgtc ggcagcaaca accaggacta cgatgtccgt tgcctgcgca ccacgtgcac
    10141 gcattgaagt aaacgcggcg tgccccgggg tgtctaggaa ggtgatcatg ccgttttcag
    10201 tttcaacgtg gtatgcacca atgtgctggg taatgccgcc cgcttcgcca gaggcaactt
    10261 tcgttgaacg aatgtagtcc agcagagagg ttttaccgtg gtcaacgtga cccatgatgg
    10321 tcacaaccgg cgcgcgcggt tcagccgcag cacccgtgtc acggtcgctc attaccgcct
    10381 cttccagctc gttttcacga cgcaggataa ctttgtggcc catctcttca gcaaccagct
    10441 gtgcggtttc ctgatcgata acctggttga tggttgccat tgcgcccagt ttcatcatcg
    10501 ctttgatgac ctgagagcct ttaaccgcca tcttgttcgc cagttcgcca acggtgatgg
    10561 tttcgccgat cacaacgtca cggttaacgg cctgagctgg cttctggaag ccttgctgca
    10621 gggaagaacc tttacgtttt ccgcctttac cgccacgtac tgctgcgcgt gcttcttcac
    10681 gatcagcttt tgattcagcg tgtttgttgc ctttcttcgg acgcgctgct ttcgcgttac
    10741 gaccacggcc acgaccgcct tcgacttcac gatcgctttc gtcttctgcc tggcgagcat
    10801 gttgagaagt agtgacgtga taatcgctgg aatcttcagt cggttccgcg ttatcagtcc
    10861 atttgttttc ttccgccata cgacgtgctt cttcagcaac gcgacgtgct tcttcttcga
    10921 gtttacgacg cgcttcttct tcggctttac gcttgagctc tgcagcttcc tgctcacggc
    10981 gggctttttc agcctgggcg tttttagtca tatcgtcttg ttgattgctc actttgtctt
    11041 tttccgcagc ttcacgtttc gcttgttcag cagcttcacg cttagcttgt tctgcagcct
    11101 cacgttcagc tttttgttgc gcctcgcgtt tagccgattc ttctgcctca cgacgggctt
    11161 gctcttccgc ttcacgctgc gcttgctctt ccgctgcaag gcgttcagcc tcttgcggat
    11221 cgcgtttcac aaaggtgcgt ttcttgcgga cttcgatttg taccgatttg ctttttccac
    11281 cggtaccagg aatgttaagg gtgctgcgtg ttttacgttg cagcgtcaat ttgtccgggc
    11341 ctgaattttt ctgattcagg tggtcaatca aagtctgttt ctcttgtgcg gacacagagt
    11401 cgtcagcaga cttccggata cctgcatcag caaattgctg taccaggcgt tccacggagg
    11461 tctgtcgctc tgcggccagc gttttaatcg ttacatctgt catgctgttc cttcctgcta
    11521 cagtttatta cgcttcgtca ccgaaccagc aaatattacg ggcagccata atcagtgctc
    11581 cggctttttc gtcggtcaac ccttcgatat cagccagatc atcaatgccc tgttcggcga
    11641 gatcttccag cgtacaaacg ccacgggcgg ccagtttgaa tgccaaatca cgatctatcc
    11701 cttcaaggtt cagcagatcg tcagccggtt tgttatcacc gaggctttct tcctgggcct
    11761 gtgcaatggt ggccagtgca tttttagcac gctcgcgcag tgcttcaacg gtcggctcat
    11821 caaggccttc gatttccaac agctctttca tcggcacata ggccagttct tccagcgtcg
    11881 agaagccttc ttctaccaga acagtcgcga agtcttcgtc gatgtcgaga tatttggtga
    11941 aggtgtcgat cgctgcgtgc gcttccgcct gatgcttagc ctgcaggtcg tcaacggtca
    12001 tcacgttgag ttcccaaccg ctcagctgcg aagccagacg cacgttctga ccgttacggc
    12061 caatcgcctg cgccaggtta ccggcttcaa cggcgatatc catggtgtgt ttatcttcat
    12121 ccaccacgat agaagcaacg tctgccggtg ccattgcgtt aatcacaaac tgcgccgggt
    12181 tatcatccca caggacgata tcgatacgct cgccgcccag ttcagtagaa accgcctgaa
    12241 cacgcgcgcc acgcatacct acacaggcac ctaccggatc gatacgttta tcgttggttt
    12301 tcaccgcgat tttcgcacga gaacccggat cgcgagccgc tgctttaatt tcaatcactt
    12361 cttcgccgat ttctggcact tcaatacgga acagttcgat cagcatttcc ggcttggaac
    12421 gagtgacgaa cagttgcgcg ccacgcgctt ccgggcgaac ggaatagagc acgccacgaa
    12481 cgcggtcgcc agggcggaag ttttcacgcg gcagcatatc ttcgcgcagg atcacggctt
    12541 cagcattgtt gcccagatcc agagagatgt tgtcgcggtt tacttttttc accacgccgg
    12601 tgatgatttc accttcgtgt tcacggaact gatcaaccac catcgcacgt tcggcttcac
    12661 gcactttctg cacgataacc tgttttgccg tctgggtagt gatacggtca aaggtaacag
    12721 actcaatctg atcttcaacg taatcgccca ggttcaggct ttcatcttca taacgtgcgg
    12781 cttcaagggt gatttccttg gtcggctggg tgacttcatc aacaactaac cagcgacgga
    12841 aggtgtcaaa atcaccgctt ttgcgatcga tctgtacgcg gacgtcgatc tcttgttcat
    12901 attttttctt tgttgctgtc gccagcgcgc tttccaatgc ttcgaaaatc ttctcgcgag
    12961 gtagcgcctt ttcattggat acggcttcaa ctacagccaa aatttctttg ttcatcgcgg
    13021 gcttttcacc tcatccagac tattaaaagt ggggaaccag gttcgccttc tggatattac
    13081 tcagcgcgaa cacttcatct ttaccttcga cggtaactgt gatcatttca ccgtctaccg
    13141 ctttgataac gccctgccat ttacgacggt tttgtaccgc catacggaga accagagtca
    13201 cctcttctcc gacaaaacgg gcgtagtgtt cagccgtgaa cagtgggcga tcgagacccg
    13261 gtgaggagac ttccaggtta taagcaacgg tgatgggatc ttcaacatcc agcacagcac
    13321 ttacctggtg gctcacatca gcacaatcat caacattgat gccatcttca ctatcaatat
    13381 agatgcgcag tgtggatgtg cgaccgcgaa taaattcgat gccaaccagt tcaaaaccca
    13441 gggcctcaac tggcgcagta atcatctctg ttaatttttg ctctaatgtg gacaagccca
    13501 cccccaagac ataaaaaaag ggcctaaagc ccagttattc tgtagtcaga taacaaaaaa
    13561 ccccgataaa tcggggcttt atataactga accctataac cgcaactgcg gtctggagca
    13621 ctttccagaa ggattttttc aaatcccact acgaaggccg aagtcttcac agtatatttg
    13681 aaaaaggact ctaagggaaa gtggttgcgg gggccggatt tgaaccgacg accttcgggt
    13741 tatgagcccg acgagctacc aggctgctcc accccgcgcc tgaaacgtgg caaattctac
    13801 tcgttttggg taaaaaatgc aaatactgct gggatttggt gtaccgagac gggacgtaaa
    13861 atctgcaggc attatagtga tccacgccac attttgtcaa cgtttattgc taatcatgtg
    13921 aatgaatatc cagttcactt tcatttgttg aatacttttg ccttctcctg ctccccctta
    13981 agcgcattat tttacaaaaa acacactaaa ctcttcctgt ctccgataaa agatgattaa
    14041 atgaaaactc atttattttg cataaaaatt cagtgagagc ggaaatccag gctcatcatc
    14101 agttaattaa gcagggtgtt attttatgac gacgattctc aagcatctcc cggtaggtca
    14161 acgtattggt atcgcttttt ccggcggtct ggacaccagt gccgcactgc tgtggatgcg
    14221 acaaaaggga gcggttcctt atgcatatac tgcaaacctg ggccagccag acgaagagga
    14281 ttatgatgcg atccctcgtc gtgccatgga atacggcgcg gagaacgcac gtctgatcga
    14341 ctgccgcaaa caactggtgg ccgaaggtat tgccgctatt cagtgtggcg catttcataa
    14401 caccaccggc ggcctgacct atttcaacac gacgccgctg ggccgcgccg tgactggtac
    14461 catgctggtt gctgcgatga aagaagatgg cgtgaatatc tggggtgacg gtagcaccta
    14521 taaaggaaac gatatcgaac gtttctatcg ttatggtctg ctgaccaatg ctgaactgca
    14581 gatttacaaa ccgtggcttg atactgactt tattgatgaa ctgggcggcc gtcatgagat
    14641 gtctgaattt atgattgcct gcggtttcga ctacaaaatg tctgtcgaaa aagcctactc
    14701 cacagactcc aacatgcttg gtgcaacgca cgaagcgaag gatctggaat acctcaactc
    14761 cagcgtcaaa atcgtcaacc caattatggg cgtgaaattc tgggatgaga gcgtgaagat
    14821 cccggcagaa gaagtcacag tacgctttga gcaaggtcat ccggtagcgc tgaacggtaa
    14881 aacctttagc gacgacgtag aaatgatgct ggaagctaac cgcatcggcg gtcgtcacgg
    14941 cctgggcatg agcgaccaga ttgaaaaccg tatcatcgaa gcgaaaagcc gtggtattta
    15001 cgaggctccg gggatggcac tgctgcacat tgcgtatgaa cgtctgttga ccggtattca
    15061 caacgaagac accattgagc agtatcatgc acatggtcgt cagttgggcc gtctgctgta
    15121 ccaggggcgt tggtttgact cccaggcgct gatgctgcgt gactctctgc aacgctgggt
    15181 tgccagccag atcactggtg aagttaccct ggagctgcgc cgtgggaacg attattcaat
    15241 cctgaatacc gtctcagaga acctgaccta caagccagag cgtctgacga tggaaaaagg
    15301 cgactcggtg ttctcgccag atgatcgtat tggtcaattg accatgcgta atctggatat
    15361 cactgatacc cgagagaaac tttttggtta tgccaaaact ggcctgctct cctcctctgc
    15421 cacttcaggc gtgccgcagg tggagaatat ggaaaacaaa ggccagtaat tcgacatcaa
    15481 ccctgccctt caggggtggg gttaccttgc ttatcctctg taaactcctt tctgagcatc
    15541 acataatcga aaacatcatg gtttgcatcc acaacccgtg agtctccttt atagtgtgca
    15601 atcacttcct caagcgtttg cggctgttcc ggctttgtta cccccatcca cgcattaata
    15661 aggtaattat tgtaagctgt cgaaaagatg ttgttttccg ttgtgcgatc cacgccatcc
    15721 ccaagaacgg ggctatacca gataaacatc gggacatgat atgcctgctg gctagcctcc
    15781 ctgcctccat gaaaatagac gttcttcttc gtagggtcac gttccaggcc gtggtcggca
    15841 aaatacatga ccgaggcgcg gcgatctttt aataattcaa aaacctgacc tagcaaacta
    15901 tctgtgtaat gaatggagtt gtcatagcag gcatcctgat cgtcctgcgg ttgaaacacg
    15961 gcgctggatt gcggataggc gctacaagcc ggttcatggc ttccatttaa atgaagaaca
    16021 atcagtttct tttgctgcgt attttgctgt aatgcttgcg ataaatgcgg caacaacaat
    16081 tcatcaaatc ctctgccata aactgtttcc atggcgcgca tggcgatact ggtaactgct
    16141 gtaccattct gccgaaaagc ggattgcgag cttagccaga aagtctgaaa tcctgcttga
    16201 ttagccatat taataatgtt gtcggggtaa ttatgaatgt catgactcaa aacagaatca
    16261 gcagttaaag aaaggggaac cgacagcgca gtgtaaggtg cgccgcttat tgcttgatta
    16321 aacagtttga tctgttttct ttgtgcttca acttgcggtg tcgtagagcg tgtatatcca
    16381 tacaaagaca tattgtcgac acgtacagac tcccccacaa tcaacacgta ggtatcaata
    16441 cctgtatccc tgactgataa ttgaaaatac ggcaccgtgt ttgcaattga gagtaatctt
    16501 tgatgctctt tcgctgctaa agcaaaatag ttgagattga aaaacggcgt ataggtagca
    16561 aatcgcgacg ctagtatata tggactgaac gcttttttat tttttgcatc tttataagca
    16621 aattgacaag cggaaaataa actgcccgaa atgacaatca gcaataatat tcctgtcact
    16681 tttttgtcgg caaggaaaca tcatatttta ttattactgc caaaaaaaga agggataaaa
    16741 aagcaaaggc acatagataa gggatataca tccccagcat tttgacaact tcatccggat
    16801 cactctggag cacactaatc gcaaatccat cattgaaagt tgtaccaaag gtacaccatg
    16861 aatagaggct aatactcatg tctgccgcag tcagtaacac aaatggtgcg gcaacaagca
    16921 ggcgaagcgg aagggatgat atacgtttta caaccaggat ggtcaggacg aagaacagca
    16981 atcgctgaac catatgcgcg ccggaagagg cgactaagtt cgtaatacca aaaacaataa
    17041 cacaaagata caacaaccaa tgagatttaa aacttctagc aaatttgttg aatactgtca
    17101 tccagaaatc caagttcaca accaatataa acaattatca acaatgttca attttatatc
    17161 agacatgttt ttcaactctg atccatatca gaccgaaaca ctgttagttt tttcttttta
    17221 aatgatgcgg ccattgcgtt ctcagtaatt aaataacatt atcaatgcgt ctctaaacat
    17281 tacgcagaac gtagccatca gtagttatac tgacaggagc aggatggttt atcgagaagt
    17341 aaacatatcc gtattcgtac cggaactgga gttgacacaa taaagtgcca attatgtcag
    17401 cagaagggaa aagtttataa ctaaggcgta ccggcaccat cgtttcaagg taccagctac
    17461 gagtaaagca actggacgag atacagatac ctgacaacca ttcctcagac caggaccaaa
    17521 acgaaaaaag acgcttttca gcgtctcttt tctggaattt ggtaccgagg acgggacttg
    17581 aacccgtaag ccctattggg cactaccacc tcaaggtagc gtgtctacca attccaccac
    17641 ctcggcacgg atactacttt ttagttcggg atatcgctgg tcggcttagc cggagcagct
    17701 ggttgagttt gttcggtttt caccggtgca ctcagatttt cccattcgct acctttattg
    17761 gttttgttgc tattgatgtt acccagcacc agactgatga tgaagaataa cgttgccagc
    17821 agcgccgtca tgcgggtcat gaagttacca gaaccacttg aaccaaacag cgtagcggaa
    17881 gcgcctgctc cgaaggaggc tcccatatca gcgcctttac cttgctgcag catgatcaga
    17941 ccaacaaggc caattgccac aataaggaaa actactaaaa gagcttcgta cataatcaac
    18001 ctgttccttg cggagttgcc gcgtaccaat gcttcaacca ataaagcggg agtttttaat
    18061 ctttcccact gaagcgggtg tgaatactaa ccaaagcgaa tgaccttcgc aagggcaatt
    18121 ttatcgcatt gtatcaactg cggaaaaaaa cagcaaaacc gatgtgttcg ctgagaaaaa
    18181 aggcgacaat cgccgccttt ttagccagtt atctaacgct ttaaacggct tttactgcat
    18241 cggcgatgcg gtgtgcaaat tcagtcacct gcgcttcgtc ttcgccttcc accatcacgc
    18301 gaattaacgg ttcggtgccg gatttacgca gcaacacgcg tccacggttg cccagcgcag
    18361 cttcaacctc tgcggtcacg gctttaactg actcatgctc aagtggatcg ccgctacctg
    18421 cggtgtaacg tacgttaacc agaatctgcg ggaacatttt catgccgctg caaaggtcgt
    18481 gcaggctcat atggttacgt gccatcgccg ccagcacctg caagccagca acgatgccgt
    18541 caccggtagt agttttatcc agtaggatca catgaccgga attctctgca ccgatacgcc
    18601 agcctttctc ctgcattttt tccagtacgt agcggtcacc cactttcgcg cgcgcaaatg
    18661 gaattcctaa ctgtttcagc gccagttcaa gccccatgtt gctcatcaat gtacccacag
    18721 cgccaccacg cagctggccc tgacgaagac cttcacgcgc gatgatatac atgatctgat
    18781 cgccatcgac tttattgcct tcatggtcga ccataatcac gcgatcgcca tcgccgtcga
    18841 aggcaatacc gagatccgct ttttcagcca gcacacgagc ctggagcgcg cgaacgtcgg
    18901 tagcccccac ttcggcattg atgtttacac cgtttggctc acaaccgata gcgataacgt
    18961 tcgcccccag ttcgcgcagc acgttcggcg cgatgtgata agtcgcaccg tttgcacaat
    19021 ccaccacaat cttcagttca ctgaggctaa gttcgttcgg gaacgtggct ttgcaaaact
    19081 cgatatagcg acccgcggca tcaacgatac ggctggcttt acccagttct gccgaatcaa
    19141 cgcagctgat ctccttttcc atttccgctt cgatggcctc ttctaccgca tccggcagtt
    19201 tggtgccgtc gatagagaag aatttaatcc cattatcgta gaacgggtta tgcgatgcag
    19261 atatcacaat tccggcctct gcgcggaagg tacgcgtcag ataagccacg gccggtgttg
    19321 gcatcgggcc agtgaagagt gcggaaaggc ccgctgccgc cagacccgct tccagtgctg
    19381 actccagcat atagccagaa atacgcgtgt ctttaccaat aataatctta cgggagccgt
    19441 ggcgcgccag cactttaccc gcggcccaac ccagcttaag cacaaaatca ggtgtgatcg
    19501 gcgcatcccc tacacgacca cgaatcccat cggtaccgaa atatttacga ttactcatag
    19561 cgtttgtttt cctttgcaga cagagtggct tccaccaccc gcatcgcttc tacggtttct
    19621 ttgacgtcat gaacacgaat gatgtgcgcg ccttgcattg cggcaatgac cgcacaggcc
    19681 agactaccgc tcaggcgctc ggacggcccc acgttcagca gctgcccaat catcgatttt
    19741 cgtgacatac ccaccaacag cggcaggttg aaatggtgaa attcagccag gcgcgccagt
    19801 aatgaatagt tatgggagag atttttaccg aaaccgaatc cggggtcgag caacaatttc
    19861 tcttttgcga tacccgcctg ctcgcaacgt gctatttgct caataaagta gcgattcact
    19921 tctgcaaaga catcgtcata cttcggagct tcctgcatgg tttttggatt tccctgcata
    19981 tgcatcagac aaaccggtaa accggtttct gcagccgcct ccagagcgcc aggttcggaa
    20041 agggagcgga tatcattaat aatgtgagcg ccaactttcg ctgactcacg gatgacttct
    20101 ggtttggatg tatcgaccga gatccagact tcgaagcgtt gagcaattgc ctcaaccaca
    20161 ggaataacac gttgcaactc ttcttcaacg ctaacttccg ccgcccctgg gcgcgtggac
    20221 tcgccaccaa cgtcaatgat cgtcgcgcca gcattgatca tcagattcgc atgtttcacc
    20281 gcatctatca gcgagttatg cgtgccgcca tccgaaaagg aatcaggcgt gacgttgagg
    20341 atccccatta cgtgaggatg gctaaggtcc agtgaagtac cctgggcaaa gagtttcatg
    20401 atgttatccc tggtatgaat tgataagaaa aaaccccgga gcacgccccg gggttttcgg
    20461 tacaaataca gtcatctgat gcgggaactt acttgtcgcc taactgctct gacatggtgt
    20521 tacccgggtt cggcgtacgc ggttcatcaa ccggacgagg agcctttgga ctaccattgt
    20581 cgccagaatt gttagaagcg cctggttctt cccagcccgc tggcggacgt acatcgcgac
    20641 gtgccatcag gtcatcaatc tgcggtgcgt cgatagtctc atatttcatg agagcatctt
    20701 tcatcgcatg cagaatatcc atattgtcgg tcagaagctg acgcgcacga ttatagttac
    20761 gctcaatcag tgctttcact tcctggtcga tgatacgtgc agtttcatcg gacatatgtt
    20821 tcgctttcgc tacgctacgg ccgaggaata cttcaccttc ttcttccgcg tacagcagtg
    20881 gacccaattt ctcagagaag ccccactggg tcaccatgtt acgtgccaga ttggtcgcaa
    20941 ctttaatatc gttggacgca ccggtagaaa catgttccgg cccatagatg atctcttctg
    21001 ccagacgacc accgtacagc gtagaaatct ggctttccag tttctgacgg ctggcgctga
    21061 ttgcgtcgcc ctcaggcaag aagaaagtca cacccagcgc acgaccgcgt gggataatcg
    21121 tcactttgtg caccggatcg tgttccggca ccaggcgacc gataatcgca tgacccgctt
    21181 cgtggtaagc cgttgattct ttctgcgctt ccgtcatcac catggagcga cgttccgcac
    21241 ccatcatgat tttgtctttc gctttctcga actcaaccat cgacacaacg cgtttgttgc
    21301 cacgagcagc gaacagtgcc gcttcgttca ccaggttcgc caggtcagca ccggagaaac
    21361 caggagtacc acgggcaatg attgccgcgt cgatatcggg ggccaatggt acgcgacgca
    21421 tgtgaacttt caggatctgc tcacgaccgc gaacatctgg caagccgacc acaacctgac
    21481 ggtcgaaacg gccaggacgc agcagggccg ggtcgagaac gtccggacgg ttagtcgcgg
    21541 cgataacgat gataccttcg ttaccttcga agccatccat ctcaaccagc atctggttca
    21601 gagtctgttc acgttcatcg tgaccaccgc ccagaccagc gccacgctgg cggcctacgg
    21661 cgtcgatttc atcgataaag atgatgcacg gtgccgcttt cttcgcctgt tcgaacatgt
    21721 cacgaacacg ggatgcaccc acaccgacga acatttctac gaagtcagaa ccggagatag
    21781 taaagaacgg aactttcgct tcgcctgcaa tcgctttcgc cagcaacgtt ttaccggtac
    21841 ccggaggacc gaccatcagg acgcctttcg ggatcttacc gccgagtttc tggaagcggc
    21901 tcggctcgcg cagatactct accagttcag caacttcttc ttttgcttcg tcgcagcccg
    21961 caacgtcagc aaaggtcgtt ttgatctgat cttccgtcag catgcgcgct ttgctcttac
    22021 caaacgacat ggcacctttg ccaccgccgc cctgcatttg acgcatgaag aagatccaga
    22081 caccaatcag caacagcatc gggaaccagg agatgaagat agaagccagc aggcttggtt
    22141 cttcaggcgg ttcaccgaca accttgacgt tcttggtcaa caggttatcc agtaatttcg
    22201 gatcctgaac cggaatgtaa gtggtataac ggttactatc tttcttggta acgttgattt
    22261 cacgtccgtt gatacgcgct tcacgaacct ggtcgttatt gacctcttgt aggaaggtag
    22321 agtaatccac cttacggcca ttagactcgc tgggcccaaa gctctggaat actgacatca
    22381 gcacaacggc aatgaccagc cagagtatta ggtttttcgc catgtcactc aagggattaa
    22441 cctcttatta caactgtgtt aaaaacagcg tcaggatact ctatatccag catctttcaa
    22501 actttcgtct gaaatctccc ggttagggtt tacgcccggt cgctacaata tacacttccc
    22561 gcgaacgtgc acgagaagag tccggcttac gaactttgac cttcgtaaac agggagcgaa
    22621 tttcccttag atactcatcg aaaccttcgc cctggaacac cttcactaca aaactgccac
    22681 ctggcgctaa tacatcacga cacatttcta gcgccagttc caccagatac atggcacggg
    22741 ggatatccac cgccggtgtt ccgctcatgt ttggtgccat atcggacatg acaacctgga
    22801 ctttgctgtc gccaacgcgc tccagcagtg ctttcatcac cagttcatca cgaaaatcgc
    22861 cctgaagaaa gtccacacca acgataggat ccataggtaa aagatcgcaa gcgatgatgc
    22921 ggcctttgcc gccaatttgg gtgaccacat attgtgacca accacccgga gcagcaccaa
    22981 ggtcgacaac cgtcattccc ggtttaaaga gtttgtcact ttgctgtatt tcatcaagtt
    23041 taaaccaggc acgggaacgt aacccctttt tctgtgcctg ttgaacatat ttatcgctaa
    23101 agtgttcctg aagccagcgg ctggagctgg cagaacgctt cttacctgtc atttaacttt
    23161 cccatgggga taactcatcg taaccaattg cgtaaatttt tacgcgccta tttggtgata
    23221 tatgggagat ggcggtagaa tgacccgttt tcaatcccaa cgtaagcaaa aaatacgatg
    23281 aatctgagta ctaaacaaaa acagcacctg aaaggtctgg cacatccgct caagccagtt
    23341 gttctgcttg gcagtaatgg tttgaccgaa ggggtgctgg ccgagattga acaagcgtta
    23401 gagcaccatg aactcatcaa ggtgaaaatc gccaccgaag atcgcgaaac taaaaccttg
    23461 atcgtggaag ctatcgtgcg cgaaaccggc gcctgtaatg tacaggtcat cggtaaaacg
    23521 ctggtgcttt atcgcccaac taaagaacgt aaaatctcgc tgccacgcta agattatcct
    23581 aaagttacac acatttgctg tgtaaaacga ggggttttcc gcaggcagga gagcaaaatg
    23641 ccacgctctg ttcgttgata aaaggccgca tagcggcctt tttcctttct ttacaataca
    23701 tcaacatctt gagtattggg taattcttac aggtattcca ccttaattac ttcaaattct
    23761 acttcgccgc ccggcgtttt gatgaccaca acatcatctt cttctttgcc gatcaggcca
    23821 cgagcaatag gcgagtttac agaaatcagg ttttgtttaa agtcagcttc gtcatcgcca
    23881 acgatgcgat aagtctgttc ttcgtcagaa tccagattca gcaccgttac ggtagcacca
    23941 aaaataacgc gcccattgtt gggcattttg gtgacatcaa tcacctgcgc gttcgacagc
    24001 ttggcttcga tgtctttaat acggccttcg cagaaaccct gctgttcacg agctgcgtgg
    24061 tattcggcgt tttctttcag gtcgccatgc tcacgcgctt ccgcgatagc agcaatgatt
    24121 tcaggacggc gcacagattt cagaaaatcc agctcttcgc gtaatttttc agcgccgcgt
    24181 aaggtcatcg gaatagcttg catttgttat acctcttgaa tattcctgat ggggcaagtc
    24241 ttcacccacc ccggctgttc ggcctgcccg gcataactcc tcaccgggac cagaagcaaa
    24301 aaaataccga cccgggtaca agtcccaggt cagctacaat tcacattttg atagtcattt
    24361 taccctgaag ttcccgaagg gtcatcgttt actttatagg gcgttgcgcc gtagtatgac
    24421 ggctcgattc caggttgtta gcgcgagatt atgcgatttt ccagatttat catcggattg
    24481 accagctgta tagcgttcag tgttcaggcc gcgaatgttg atgagtacgt tactcaactc
    24541 cccgctggtg ccaaccttgc cctgatggtg caaaaagtcg gcgcgtcggc ccccgctatt
    24601 gattaccaca gtcagcagat ggcgctgccc gccagtacgc agaaagtgat tactgcgctg
    24661 gcggcgttga ttcaactcgg ccccgatttt cgtttcacca cgacgcttga aaccaaaggc
    24721 aatgtggaaa acggcgtact taagggtgac ttagtggcgc gatttggtgc cgatccgacg
    24781 ttaaaacgtc aggatattcg caatatggtc gcgactttga aaaaatctgg cgtcaaccaa
    24841 atcgatggca atgtgttgat agatacctcc attttcgcca gccacgataa agcccccggc
    24901 tggccatgga atgacatgac acaatgcttt agcgctccgc ctgccgctgc catagttgac
    24961 cgcaactgtt tctccgtctc gctctacagt gccccaaaac ctggtgatat ggcttttata
    25021 cgcgtggcat cttattaccc cgttacgatg ttcagccagg tacgcaccct cccccgaggt
    25081 tctgccgaag cgcaatactg cgaactggat gtcgtaccag gcgacctgaa ccgctttacg
    25141 ctgacgggat gcctgccaca acgttctgag ccgctcccgt tggcttttgc cgtgcaggat
    25201 ggagccagct atgccggggc aattctgaaa gatgagttaa aacaggcagg tatcacctgg
    25261 agcggaacac tgctgcgcca gactcaggtt aacgaacctg gaacggtagt tgccagtaaa
    25321 cagtcggccc cgttgcacga tctgcttaag attatgctga aaaagtcgga caacatgatc
    25381 gccgatacgg ttttccgcat gataggccat gcgcgcttca atgtgcctgg aacatggcgg
    25441 gccgggtcgg acgccgtgcg tcagatcctg cgccagcaag ccggtgtcga tattggaaac
    25501 accattattg ccgatggttc agggctttcg cggcataacc tgattgcccc cgccaccatg
    25561 atgcaggtgt tgcaatacat tgcccaacac gacaatgaac ttaactttat ctccatgctg
    25621 ccgctggcgg gctatgacgg ctctttgcag taccgtgcag gtctgcatca ggcgggcgtg
    25681 gatggaaaag tctcagcgaa aaccggttcg ttgcaggggg tatataacct ggcgggattc
    25741 attaccacag cgagcgggca acgaatggcg tttgtacaat atctttctgg ctatgcagta
    25801 gaaccggcgg atcagcgtaa tcgccgtatt ccgttagtgc gttttgaaag ccgtttgtat
    25861 aaagatattt atcagaacaa ttagtcaaaa agaaaccccg gcacatggcc ggggttttgc
    25921 tgattaacgc ttgtaaatga actcaacgcc ttcttcgtcg tcttcgtccc agtcatcatc
    25981 ccagtcctca tcatcctctt cagcaatctc ttcaagctgc tggcgatgat aatcatccca
    26041 catgaattcg actttctctg gctgtttcgc ttcttcagcc tgcacgaccg ggttttcaat
    26101 gataaaggtc atcacatccc agcagagatc tttcacgccc agtccactcg ccgcagagat
    26161 cagataatat ttatcttccc agcccagcgc ttcagcaatc gctttcgctt tctcttcagc
    26221 ttctgcctta tccagcagat cgatcttatt gaacactaac caacgcggtt tcgccgccag
    26281 atcctggctg tatttttcca gctcgctgat aataatacgc gcgttttcaa ccggatcggt
    26341 gccgtcaatc ggatcgatat cgatgaggtg caacaggacg cggcaacgtt ccaggtgctt
    26401 caggaagcgg atacccagac ctgcgccttc cgcagcgcct tcaatcagtc ctggaatatc
    26461 ggcaacaacg aagctctttt cgttgtccat tcgtaccaca cccagacttg gcaccagagt
    26521 ggtaaacgga taatccgcca ctttcggttt agccgccgat accgcgcgaa taaaggtcga
    26581 tttacccgcg tttggcatcc ccaacatacc gacgtcagcc agcagcatca gctccagcag
    26641 cagctcacgc ttatcacccg gcgtaccgtt ggttttctgc cgtggtgtac ggttaacgga
    26701 cgatttgaaa cgggtattgc ccagaccgtg ccagccgccc ttagcaacca gcagacgctg
    26761 accgtgttta gtcatatcgc ccatggtttc accagtaccc tggtcgatta cacgcgtacc
    26821 taccggcact ttaatcgtca cgtctttacc gcgcttaccg gtacaatcgc ggcttgcgcc
    26881 gttctgaccg cgctctgcac ggaaagattt ttcaaaacga taatcgataa gcgtgttcag
    26941 gttttcgtcg gcttccatcc atacgtcacc accatcacca ccgtcgccgc catccgggcc
    27001 gcctttcgga atatactttt cgcggcggaa gctcacgcaa ccattaccgc catcacctgc
    27061 aacgaccaga atcgatgctt catcaacaaa cttcatttta ttctccgtaa atcattcgcc
    27121 tgcgcggggt tgcgatacca ccgttgtatg cttacgtaat ccgccccaaa tacgatgacc
    27181 aatggcggaa tacattgcgc ccgcaaccac gacaaacgca ccgagataac ctaaaaggtt
    27241 taacatcggt ctggcgaaga aatcgggcca ggccagtgat aaaagatctg aaaaaaacag
    27301 cgtaaacagt ggggtgagcg tgatgatcgc gctcacctgc gctgcctgcc agcgagccat
    27361 cgcttccgcc agggcgccat atcctaccaa tgtattcagt ccgcaaaaaa ttaaacatgc
    27421 gagctgccag tggctaagct gcgctatcac tccaggcttt gccagaggga agagcgcaat
    27481 tgtacataaa gtgtacagta aaaacaggat ctgcggtgag gccagccgac gcaataaaac
    27541 cttttgcgcc acgccataac tcacccaaac cgtcgccgca ccgaccccaa agataactcc
    27601 ccaggtgtaa tcggtgagct ttgtaaatat ctcgaccaga ctggtgttaa aaaacatcac
    27661 caggccgctc aggagcatca atgcccctac aacctgagtg ctgcgcattt tctctttcag
    27721 gataaatacg ctggcaacca tcatgccaac tggcgagagt tgcccaatca cctgcgaagc
    27781 ggtcggactc aggtattgca aggatgagct gaacaggatg aagttcccaa acagcccggc
    27841 ggtcgccact gccaacaaaa tcaaccagcg tggcttacga aacacgcgta atggcggcaa
    27901 cctcttcttc accgcaagaa tggcaccaag gccaatactc gccatcaaga aacggtaaaa
    27961 cacgattgtc ggaggttcca tcacctccag cacctgcttc attgcgattg gcaacgcccc
    28021 ccagcaaatt gctgtggtga gcgccaaaag aatgccaatg cctgcctgct gcttcatacc
    28081 gtattccctg caaagcgcat ttgcggatta acgtcgcacc atgcgcattt ttaccggtta
    28141 tcgaatgtaa aaagccccgc aacgtgttgc ggggctttca tccgttaccg ggacgcgaaa
    28201 aacttattca gcttcgatgc tgataaattt acggtttttc gggcctttaa cttcgaattt
    28261 cactttaccg tctgctttag caaacagagt gtggtcacga ccgcaaccta cgttagcgcc
    28321 agcgtggaat ttggtaccac gttgacgaac gatgatgcta cccgccagaa cggattcgcc
    28381 accgaaacgc ttaacgccca ggcgtttagc ttctgaatcg cgaccgttac gtgtggagcc
    28441 gccagccttt ttatgtgcca tttgaaatct ctcctcaggt cttaggcgct gatgccagta
    28501 attttcacat cagtgaacca ctgacgatgg ccctgctgct tacgatagtg tttacgacga
    28561 cgaaacttaa cgattttaac tttctcgcca cgaccgtgag caacaacttc agctttgatt
    28621 acgccgccat caacgaaagg aacgccgatt ttgacttctt caccgtttgc gatcatcagc
    28681 acttcagcga actcaacagt ttcgccagtt gcgatgtcca gcttttccag gcgaacggtc
    28741 tgaccttcgc ttactcggtg ttgtttacca ccactttgga aaaccgcgta catataaaac
    28801 tccgcttccg cgcacacctt ttcaatgatt cagagtgcgc tataaatatt cacaataggg
    28861 cgcgaatatt acgcaaaacg cacgcctttg acaagtgcta cagtcaatac acgaagaaaa
    28921 aaaacacaac ttgtacggta acgtttatct gtgccatttt ttcagtacaa tcactctata
    28981 ttcctaacca taaaccctaa gttgcctttg ttcacagtaa ggtaatcggg gcgaaaagcc
    29041 cggcttttgc gatgaattta gaaaaaatca atgagttaac cgcgcaagat atggcgggtg
    29101 ttaatgcggc aatccttgag cagcttaatt ccgacgtcca gctgatcaat cagttaggct
    29161 attacatcgt cagcggcggc ggtaaacgta ttcgtccgat gattgctgta ctggctgcac
    29221 gagctgttgg ctatgaggga aatgcgcatg tcaccattgc tgccctgatc gagtttatcc
    29281 acacggcgac tctgctgcac gacgacgttg tggatgaatc agatatgcgc aggggtaaag
    29341 ctaccgccaa cgctgcattt ggcaatgccg ccagcgtgct ggtaggcgat tttatttata
    29401 cccgcgcgtt ccagatgatg accagcctcg gttcgctcaa agtgctggaa gtcatgtcag
    29461 aagccgtaaa cgtcatcgca gaaggtgaag ttctgcaact gatgaacgtt aacgatccgg
    29521 acatcactga agaaaactac atgcgcgtta tctatagcaa aaccgcgcgt ctgtttgagg
    29581 ctgccgcgca gtgttccggg attctggctg gctgtacgcc ggaagaggag aaaggcctgc
    29641 aggattatgg gcgctatctc ggcactgctt tccagttgat cgacgattta ctcgattaca
    29701 atgccgatgg cgaacagtta ggtaaaaatg tcggcgacga tctgaacgaa ggtaaaccga
    29761 cgctgccgct gctgcatgcg atgcatcatg gcacaccaga acaggcacag atgatccgta
    29821 ccgccatcga acagggtaac ggtcgccatc ttctggaacc ggttctggaa gcaatgaacg
    29881 cttgtggatc tcttgaatgg acgcgtcagc gtgccgagga agaagcagac aaagccatcg
    29941 cagcgttaca ggtgctcccg gacacccctt ggcgagaagc actcatcggc ctcgcgcaca
    30001 tcgctgttca acgcgatcgt taatcccctc ccctcatccc gcgcagcgcg cggggtggtt
    30061 ccagtaaatt ccataaaaac acttactcag ctctataaca cctgaaaaac aggctgtgca
    30121 tctttatata ttctgaatat tcacactcct tacaggaact ttttagagca atcggccatc
    30181 aggagtatag tgatgctcga cagaagaagt gttctgaatg aaagcgacta acttaaggag
    30241 tgaggaaaga tggaaagtaa tttcattgac tggcatcccg ctgacatcat tgcgggtttg
    30301 cgcaagaagg gaacgtcaat ggcggcggag tctcgcagaa atggtttgag ttcctcaacg
    30361 ctggcgaatg cattatcgcg cccatggccg aaaggagaga tgattattgc gaaagccctg
    30421 ggaactgacc cctgggttat ctggccatca cgctaccatg atccgcagac ccatgagttt
    30481 atcgacagaa cgcagttgat gcgtagctac actaaaccga aaaaatgagt ggtctggcgg
    30541 tagccccgcg aacggggctg ccagctctca gacgattatt cgcctttcac acgctcaata
    30601 tttgcaccta aagcgcgcag tttgtcttca atgcgttcgt agccacgatc gatgtgataa
    30661 atacgatcaa ccaccgtcgt cccttccgca atacatccag ccagcaccag gcttgctgat
    30721 gcacgcagat cggttgccat aacctgtgcg ccagaaagtt tttcaacacc gtgacaaata
    30781 acggtattgc tttcgatttc ggcatgcgcg cccatacggc taagctccgg cacatgcata
    30841 aagcggtttt caaagaccgt ttcggtgatg aatccggtcc cttctgccac caggttcaac
    30901 agcgtgaact gggcctgcat atcggtcggg aatgccggat gcggcgcggt acgtacgtta
    30961 acagccttcg gacgtttgcc atgcatatcc aggctaatcc agtcttcgcc gacttcgatg
    31021 tccgctccag cgtcacgcag tttcgccagc acggcgtcga gagtatctgg ctgcgcgtta
    31081 cggcagataa ttttgccgcg agaaatcgcc gccgccacca ggaaagtacc ggtttcgata
    31141 cgatccggta gcacgcgata gacaccgccg cctaaacgtt ccacaccttc gatgacgata
    31201 cgatcggtgc cctgaccgct aattttcgca cccagcgtaa tcaggaagtt cgcggtatcg
    31261 acgatttccg gttcacgcgc tgcgttttca ataatcgtgg taccttccgc cagggttgca
    31321 gcacacatga tggtcaccgt tgcgccaacg ctgactttat ccatcacgat atgtgcgcct
    31381 ttcagacgac catcgacgga agctttaacg taaccttctt ccagtttgat ggtcgcgcct
    31441 aattgttcga ggccagaaat gtgtagatca accggacgcg caccgatcgt acaaccgcca
    31501 ggcagtgaaa cttgcccctg accaaagcgc gctaccagcg gccccagtgc ccagatagaa
    31561 gcacgcatgg ttttaaccag atcgtaaggt gcgcagaata cattaacgtc gcgggcatca
    31621 atatgcacag aaccattacg ttctactttc gcacccagct ggcttagcag cttcattgat
    31681 gtatcgacgt ctttcagttt tgggacgttc tggatctcta ccggttcttc cgccagtaga
    31741 gcggcaaaaa ggataggcag agcagcattt ttagcgccgg aaattgtgac ttcgccctgg
    31801 agcttcgttg gcccctgaac acgaaattta tccatttagt ttgttctcag ttaacaattc
    31861 atatccgcta ccggcgaatc gcccatagct caaaagccgt tcagtttgcg atcgcgcgcc
    31921 cactccgcag gggtatacgc tttgatcgac acagcatgaa tgcggttatc cgcaatatat
    31981 tccatcagcg ggccatagac cgtctgctgt tttttaaccc gactcatgcc gtcaaacaac
    32041 tcacccacgg caataacctg aaagtggctg ccatcgccgg aaacgtggac ttcctggagg
    32101 gagagagcgt tcatcaacac gctctgaatt tcattatttt ccatgggatc ttcaatcatc
    32161 agttaataaa ccagcgaaac atcttagagc aaagttgcgc tggcataaat aagcaaaaag
    32221 cctcgctgat aaatcagaca aggctcgact tgcaggcagg tttgccggac aggcggttaa
    32281 cgccatatcc ggcctgaaaa aatttaacga ggcagaacat cagcaggcaa attatacaat
    32341 ttcgccaggg tatacacttt gtcgtttacc ccctgaagcg tcacattgtt gccctgcttt
    32401 ttcgccagat cgataagatg gagcagcagt gccagtcccc ccgtatccac gcgggacaca
    32461 cggctaagat cgatgcaggt aatccccttc accgcttcct cacgcatttc ccaaagcggt
    32521 agcaaaacgt cctgatccag ctctccggat aacgccagcg tgtcacccgt ctgcatccag
    32581 ctcagtgact cgctcattat tttttctctt ccagagtgat tttctgttga gaaatcgatt
    32641 tcagttgcgc agtcaggccg tcgatacctt tggtacgcag cagcgttccc cactcgtttt
    32701 gtttggtggt gatcatactg acgccttcag caatcatgtc gtaagcctgc cagttgcccg
    32761 tctgggagtt tttacgccac tggaagtcca gacgcaccgg cgggcggcca ttcggatcaa
    32821 taatggtaac gcgaatcggc acaatggttt tatcgcccag cggctgttct ggcgcaatct
    32881 gataggtttg accgtgatac atcgccagcg cctgaccgta agcctgcttc aggtactcac
    32941 ggaaagcggc aaagtaggct tcacgttgag caggggtcgc actcttgtaa tactggccca
    33001 gcaccagcgc accggcgtat ttcacctgta cgtatggcag cagttcctga tcaacaatgg
    33061 tacgcagata atccgggttg gcccgaattt gcggttgctc attcttcagg cgatcgaacg
    33121 ttttctgcgc cgcctcgtcc atcagcttat aaggattggt ctggtctgcc gcggttgccg
    33181 cactcagagg tgcaatcacc agcaaagcga ccatcattaa acgtttaaac atgcgtcggt
    33241 tctcctgaaa ttatttcgtt gtacccacag gttcagtggt ttcattatta cctggcgcag
    33301 cagctggcgc atcgccacta ttcttattgt catcgccttt actaccgtaa aggaactgac
    33361 caatgagatc ttccagcacc atcgcagact tagtgtcctg aattgtatcg ccatccttca
    33421 ggatagcagt ccccagttcc gggtcttcaa aaccgacgtt taatgccaga tattgttccc
    33481 ccagcaggcc ggaagtacga atgctcagcg aactggtatc tggaatgtgg ttataacgtt
    33541 gttcaatttc cagcgttacg cgcggcagat aggttttcgg gtccagcgta atatccgcca
    33601 cccgacccac aacaacgcca ccaatactga ccggagagcg cgctttcagg ccgccaatgt
    33661 tatcgaacgt cgcataaagc gtgtaggtcg gttcagtacg tatggacgtc acgttcgccg
    33721 ccttcaggca aacaaacagc gccgccagca gtgctgctaa taaaaagata cccacccaaa
    33781 tttcattttt tttcgtttgc atgaactcaa ttcccaaaca tcaatgcggt cagcacaaaa
    33841 tccagcccca gaacagccag agacgagtgg acaacggtgc gagtggttgc ccggctaatc
    33901 ccggcagacg tcgggatggc gtcgtagccg ttaaacaacg aaatccacgt caccgtgatg
    33961 gcgaacacca cgctcttaat cagacagttg accagatcca tacgccagtc gacggcattt
    34021 tgcattgccg accagaagaa cccgctatca atgcctttcc aactgacgcc gaccagagat
    34081 ccgccccaga tccccacggc gacgaaaata accgtcaaca gtggtaatga aataacccca
    34141 gcccagaaac ggggagaaat aacccgacgc agcggatcca ccgccatcat ctccatactg
    34201 gagagttgct ctgtagcgcg catcaggccg atttctgcgg ttagcgccga accagcacgc
    34261 ccggcaaaca acaacgcggc aaccaccggc cccagttcac gcagtagcga taacgccacc
    34321 agcataccca gactggtttc cgcactataa gtggtcagaa ccagataacc ttgcagcccc
    34381 aacaccattc cgatgaacac gccagaaacc acaataatca gcatcgacag gacgccgaca
    34441 ttatagagct ggcgcaccag cagcggcgca tgtttgcgaa attccggttt gccgaccagc
    34501 gcattgaata acattaaccc ggcccgcccg aacgttctca gggttttaat ccctttatgt
    34561 ccgagcgacg ccagcgcatt taacagcatg agtggcttaa ctccctggta aaagatcagc
    34621 gtgataatcg ccggcaggat agcggaacgg aacaggcccg tcagctatcc cgtccagaaa
    34681 ctgacgtacg cgcggatcag gattcgcctg caacgcctgg gcactgccat gagcgacaat
    34741 ttttttgtcc gccaggatcc aggcgtgatc cgcaatactt aacacttccg gcacatcgtg
    34801 agaaaccacc acacaagtca cgcccagcgc gctgttcagc tcagaaatca gcttcaccag
    34861 tacgcccatg gtgatgggat cttgcccaac aaaaggttca tcaaacatga tgagatccgg
    34921 ctccagcgca atcgcacgcg ccagcgctgc acgccgcgcc atcccaccgg aaagttcaga
    34981 aggcattagt ttagccgctc cacgcagccc cacggcctcc agcttcatca tcaccgtact
    35041 atgcaacaat ggcgcgggaa gttgggtatg ttcgcgcagt ggataggcga cgttgtcaaa
    35101 tacgttcata tcagtgaaca acgccccgga ctgaaataac atgctcatcc gtttgcgcac
    35161 tgtatacagg cgcgaacgag acatcgccgg aatattctca ccatcgaaaa ggatctcacc
    35221 atgatctggt gcgatttgcc cgccaatcag acggagtagc gtcgttttac cgatgcccga
    35281 tggccccatg atcgccgtga tcttccctcg cggcacggtc agggaaatat tatcgaagat
    35341 gcagcgattg ccacgcgtaa aactgacatc gcgcatatcg actaaattcg ccacagactg
    35401 ctccataatt cacccttcgt cttgcgttga ttttctaagc atggcgctca atttaacctt
    35461 gaacccaaca tatttacaga atattacccg ccgtggttag cgaaagctgg catttgtttt
    35521 actttttagc cgcataaagt caaaattaag aatccgttac ggctttctga aaatcttcag
    35581 cggaccggcg agtatacctg aagaaaggac gttagatgct tttagctacg gcactgttaa
    35641 ttgttggttt acttttggtc gtttacagtg ccgaccgcct ggtttttgcc gcgtctattc
    35701 tttgccgaac ctttggcatc ccgccgctga tcatcggcat gacggtggtc agtattggta
    35761 catcgttacc agaaatcatc gtctcgcttg ccgcgtctct gcacgaacaa cgcgatttag
    35821 ccgttggtac agccctcggc tcaaacatta tcaatatatt gctgatcctc ggtctggctg
    35881 cgctggttcg tccttttacc gtccattctg atgttctacg ccgtgaatta cccttaatgt
    35941 tgttggtcag cgtggtggcc ggttccgtac tctatgacgg acaacttagt cgcagcgatg
    36001 gtatctttct cctctttctg gctgtgctat ggctgctgtt cattgttaaa cttgcacgtc
    36061 aggctgaacg tcaggggact gacagcctga ccagagagca gcttgcagag ctgccgcgtg
    36121 acggcggatt gcccgtcgcg tttttatggc tcggcattgc gcttatcatc atgccagtgg
    36181 ccacgcggat ggtggttgat aacgccacgg tgctggcgaa ttactttgcc atcagcgagt
    36241 tgacgatggg tctgacggca attgctatcg gaaccagcct gccggaactg gcaaccgcaa
    36301 tagcgggggt tcgcaaaggt gaaaacgaca ttgctgtcgg aaatatcatt ggcgcaaaca
    36361 tttttaatat tgtcatcgtg ttgggtttac ccgcgctgat aacgccagga gagattgatc
    36421 cactggcgta cagtcgtgac tacagcgtga tgttgctggt gagcattatt tttgcgttgc
    36481 tgtgctggcg gcgctcccca caaccgggcc gtggtgtagg ggtattatta actggcggat
    36541 ttatcgtatg gctggcgatg ttgtactggt tatcgccaat actcgttgaa taactggaaa
    36601 cgcattatgt cgcacgtaga gttacaaccg ggttttgact ttcagcaagc aggtaaagaa
    36661 gtcctggcga ttgaacgtga atgcctggcg gagcttgatc aatacatcaa tcagaatttc
    36721 acgcttgcct gtgaaaagat gttctggtgt aaagggaaag ttgtcgtcat ggggatgggg
    36781 aaatcggggc acatcgggcg caaaatggcc gcaacgtttg ccagcaccgg tacaccttca
    36841 tttttcgtcc atcctggtga agccgcgcat ggtgatttag gcatggtcac cccacaggat
    36901 gtggtgattg ctatctctaa ctcaggtgaa tccagcgaaa tcacggcctt aattccagtg
    36961 cttaagcgtc ttcacgtacc gttaatctgc atcaccggtc gcccggagag cagcatggcg
    37021 cgcgccgcag atgtgcatct gtgtgttaaa gtagcgaaag aagcctgtcc gttagggctg
    37081 gcaccgacca gcagcaccac cgccacgctg gttatgggcg atgccctcgc tgtcgcgctg
    37141 ttaaaagcac gcggctttac tgctgaagat tttgcgctct cacacccagg cggcgcactg
    37201 ggtcgtaaac ttctgctgcg cgtaaacgat attatgcata cgggcgatga gatcccgcat
    37261 gttaagaaaa cggccagtct gcgtgacgcg ttgctggaag ttacccgcaa aaatcttggt
    37321 atgactgtca tttgcgatga caatatgatg attgaaggca tctttaccga cggtgattta
    37381 cgccgtgtct tcgatatggg cgtggatgtt cgtcagttaa gtattgccga tgtgatgacg
    37441 ccggggggaa tacgtgtgcg ccctggcatt ctggccgttg aggcactgaa cttaatgcag
    37501 tcccgccata tcacctccgt gatggttgcc gatggcgacc atttactcgg tgtgttacat
    37561 atgcatgatt tactgcgtgc aggcgtagtg taaagattca aggataaaca acaatgagca
    37621 aagcaggtgc gtcgcttgcg acctgttacg gccctgtcag cgccgacgtt atagcaaaag
    37681 cagagaacat tcgtctgctg atcctcgatg tcgatggcgt actgtcagat ggcctgattt
    37741 atatgggcaa taatggcgaa gagctgaaag cgttcaatgt tcgtgacggt tatggcattc
    37801 gttgtgcgct cacctctgat attgaagtcg ctatcattac cgggcgaaag gctaaactgg
    37861 tagaagatcg ttgtgccaca ttggggatca ctcacttgta tcaggggcag tcaaataaac
    37921 tgatcgcctt tagcgatctg ctggaaaaat tggcgattgc cccggaaaat gtggcttatg
    37981 tcggcgatga tctcatcgac tggccggtaa tggaaaaagt gggtttaagc gtcgccgtgg
    38041 ccgatgcgca tccactgttg atcccgcgcg ccgattacgt gacgcgcatt gctggcggtc
    38101 gtggcgcagt gcgcgaagtt tgcgacttat tactccacgc gcagggcaaa ctggatgaag
    38161 ccaaagggca atcgatatga gtaaagccag acgttgggtt atcattgtgc tatcactggc
    38221 ggttctggtg atgatcggca ttaatatggc cgaaaaagac gataccgccc aggtggtcgt
    38281 caacaacaat gatcccacct ataaaagcga gcatacggac acgctcgtct ataacccaga
    38341 aggggcacta agctatcgat tgattgctca acacgttgaa tattattccg atcaggccgt
    38401 ttcgtggttt acgcagccgg tacttaccac gtttgataag gataaaatcc cgacatggtc
    38461 cgtaaaagca gataaagcca agctgaccaa tgaccggatg ctctatttat atggacacgt
    38521 tgaagtcaac gcacttgtgc cagactctca acttcgcaga atcacgacgg ataacgcgca
    38581 gatcaatctg gtgactcagg atgttacctc tgaagacctc gtcacgttat acggaacaac
    38641 atttaactcc agcggtctga aaatgcgcgg caacttacgc agcaagaacg ccgagctgat
    38701 tgaaaaggtt agaacatcct atgaaattca aaacaaacaa actcagcctt aatcttgtgc
    38761 ttgccagctc acttctggcc gccagcattc cggcatttgc cgtaaccgga gacactgatc
    38821 agccgatcca cattgaatcg gaccagcaat ctcttgatat gcaaggcaac gtggttacct
    38881 ttaccggtaa tgtcatcgtc acccagggca ccatcaaaat taatgccgac aaagtggtcg
    38941 ttacccgtcc gggcggcgaa caaggtaaag aagtgattga cggctacggt aaaccggcaa
    39001 cgttctacca gatgcaggac aacggtaaac ccgttgaagg tcacgcttcc cagatgcact
    39061 acgaactggc aaaagatttt gtcgttctga cgggtaatgc ttatctgcag caggtcgata
    39121 gcaacattaa gggcgataag atcacttacc tggtaaaaga gcagaaaatg caggctttca
    39181 gcgacaaagg caagcgcgta accaccgttc tggtgccgtc gcagctgcag gacaaaaaca
    39241 acaaaggcca gaccccggca cagaagaagg gtaattaatt cgttatggca acattaactg
    39301 caaagaacct tgcaaaagcc tataaaggcc gtcgcgtggt agaagacgtc agcctgaccg
    39361 tcaactccgg ggaaattgtc ggtctgctgg ggccaaacgg tgccggtaag accaccactt
    39421 tctatatggt tgttggcatt gtgccgcgcg atgcgggcaa catcattatt gatgatgacg
    39481 atatcagtct gctgcctctg catgcacgcg cgcgccgcgg tatcggctat ctgccacagg
    39541 aagcctccat tttccgtcgc ctcagcgttt acgataacct gatggcggta ctgcaaattc
    39601 gtgacgactt gtctgctgaa caacgtgaag accgcgcgaa cgagctgatg gaagagtttc
    39661 acattgagca cctgcgtgac agcatggggc agtcactctc cgggggtgaa cgtcgccgtg
    39721 tagaaattgc ccgcgcactg gctgcgaatc cgaaatttat tctgctcgac gaaccgtttg
    39781 ccggggttga cccgatctcg gttatcgaca ttaaacgcat cattgagcac ctgcgcgaca
    39841 gcggcctggg cgtgctgatc actgaccaca acgtgcgtga aacactggcg gtttgtgaac
    39901 gcgcttatat cgtcagtcag gggcatttga tcgcccacgg cacgcctaca gaaatcttac
    39961 aagacgaaca cgttaagcgt gtataccttg gggaagactt cagactctga tagggtagaa
    40021 gtttgcgacg ttttagcagg agagtacgat tctgaacatg aagcaaggtt tgcaactcag
    40081 gcttagccaa caactggcga tgacgccaca gctccaacag gcaattcgtc tgttgcagtt
    40141 gtcgacgctg gaacttcagc aggagctaca gcaggcgctg gagagtaatc cgctgcttga
    40201 gcaaatcgac actcatgaag aaatcgacac ccgcgaaacg caagacagtg aaacgctgga
    40261 caccgccgac gcgctcgaac aaaaagagat gccggaagag ctgccgctcg atgccagttg
    40321 ggacaccatt tacaccgctg gtacaccatc cggcaccagc ggtgactaca ttgacgatga
    40381 gctgccggtc tatcagggcg aaacgacgca gaccttgcag gattacctga tgtggcaggt
    40441 cgagctgaca ccgttttccg acactgaccg cgctattgct acctctatcg tcgatgccgt
    40501 tgatgacacc ggttatctga ctgtcccgct ggaagatatt ctcgaaagta tgggcgatga
    40561 agagatcgac atcgacgagg ttgaagccgt ccttaagcgg atccaacggt ttgatccggt
    40621 cggtgtagcg gcaaaagatc tgcgtgactg cctgctaatc caactctccc aattcgataa
    40681 gaccacgccg tggctggaag aggccagatt gatcattagc gatcatctcg atctgttagc
    40741 caatcacgac ttccgcactt taatgcgcgt cacgcgtctg aaagaagatg tgctgaaaga
    40801 agccgtcaat ctgatccagt cgctcgatcc gcgccccggg cagtcgatcc agactggcga
    40861 acctgagtat gtcattccag atgtgctggt gcgtaagcat aacggtcact ggacggtaga
    40921 actcaacagt gacagcattc cgcgtctgca aatcaaccag cactacgcct cgatgtgcaa
    40981 taacgcgcgc aacgatggtg acagccagtt tatccgcagc aatctgcagg atgccaaatg
    41041 gttgatcaag agtctggaaa gccgtaacga tacgctactg cgcgtgagtc gctgtatcgt
    41101 tgaacagcag caagccttct ttgagcaagg tgaagaatat atgaaaccga tggtactggc
    41161 cgatatcgcc caggctgtcg aaatgcatga atcgacgata tctcgcgtga ccacgcaaaa
    41221 atacctgcat agtccacgag gcatttttga actgaagtat ttcttttcca gtcacgtcaa
    41281 taccgagggc ggcggcgaag cttcctccac ggcgattcgt gcgctggtga agaaattaat
    41341 cgcggcggaa aacccagcga aaccgttgag cgacagcaag ttaacctctt tgctgtcgga
    41401 acaaggtatc atggtggcac gccgcactgt tgcgaagtac cgagagtctt tatccattcc
    41461 gccgtcaaac cagcgtaaac agctcgtttg acccaaccga taaggaagac actatgcagc
    41521 tcaacattac cggaaataac gtcgagatca ccgaggcact gcgcgaattt gttacagcca
    41581 aatttgccaa acttgagcaa tattttgacc gaatcaacca ggtctatgtt gttctgaaag
    41641 tggagaaagt cacccacacc tcagatgcaa cactgcatgt aaacggtggt gaaattcatg
    41701 ccagcgcgga aggtcaggac atgtacgctg ccattgatgg cttaattgat aagctggcac
    41761 gccagttgac caaacataaa gataaactga aacaacacta attgtccggg caattagcat
    41821 gtgcatggcg gcctgttgtg cggcacaacg ggccatttgt acggttaatg ctccgagcct
    41881 gttccactgt ttgagtgggc aggttcttag gtgaaattat gacaaataat gatacaactc
    41941 tacagcttag cagtgttctt aacagggaat gtacgcgaag ccgcgtccac tgtcagagca
    42001 aaaaacgcgc cctggaaatc atcagcgagc tggcggcgaa acaacttagc ctgccgcctc
    42061 aggtggtttt tgaagctatc ctgacgcggg aaaaaatggg cagtaccggt atcggcaatg
    42121 gtattgccat tccgcatggc aaactggaag aagatactct gcgtgccgtt ggcgttttcg
    42181 ttcagctcga aacgcctata gctttcgatg ccatcgacaa ccaaccggtg gacttacttt
    42241 ttgccctgct ggtgccggca gaccaaacta aaacgcacct gcatactctg tcgctggtgg
    42301 cgaaacgtct ggcggacaaa accatctgcc gccgtttacg cgcagcccag agcgatgaag
    42361 agctgtatca aatcattacg gataccgaag gtactccgga tgaagcgtag ttattcggta
    42421 atgtctcttt tagacgttgt gaggagaaac agtacatggt actgatgatc gtcagcggac
    42481 gttcaggttc aggtaaatct gtcgccctgc gtgcgctgga agatatgggt ttttactgcg
    42541 tggataacct tcccgtagtg ttgttacccg atctggctcg aaccctggcc gatcgcgaga
    42601 tttctgccgc cgtcagcatt gatgttcgta atatgccgga gtcaccagaa atattcgaac
    42661 aggcgatgag taacctgcct gacgctttct caccgcaact actgttcctg gatgccgacc
    42721 gtaatacctt aattcgtcgt tacagtgaca cgcgccgact gcatccgctt tccagcaaaa
    42781 acctgtcgct ggaaagtgct atcgacaaag aaagtgatct gctggagcct ctgcgttcgc
    42841 gagcggatct gattgtcgat acctcagaaa tgtccgttca cgagctggca gaaatgctgc
    42901 gtacccgtct gctgggtaaa cgtgaacgcg aactgacaat ggtctttgag tctttcggct
    42961 tcaaacacgg tatccctatc gatgcagatt acgtctttga cgtgcgcttc ttgccgaacc
    43021 cgcactggga tccgaaactg cgtccaatga caggtcttga taagcctgtc gccgcgttcc
    43081 tcgaccgcca cacagaagta cacaatttta tctaccagac gcgaagctat cttgagctat
    43141 ggttacctat gctggaaacc aacaaccgta gctacctgac ggtcgccatt ggttgtaccg
    43201 gcgggaagca ccgttcggtg tatattgcag agcaactggc agactacttc cgctcgcgcg
    43261 gtaaaaacgt ccagtcacgc catcgtacgc tggaaaaacg taaaccatga ccgtcaagca
    43321 aactgttgaa atcacaaaca agctgggcat gcatgcccgg cctgcaatga agctgtttga
    43381 attaatgcag ggttttgacg ctgaagtgct gttacgtaat gacgaaggca ccgaggctga
    43441 agccaacagc gttattgcgc tgctgatgtt ggattctgcc aaaggacggc agattgaagt
    43501 tgaagcgacc ggtccacagg aagaggaagc actggccgcc gttatcgccc tctttaactc
    43561 tggttttgat gaagattaat cttcatcact ttgacattca aatcccttca agctcccccg
    43621 gcaataagaa aagcgcgtac tgaaatcgtt ctcaatcaac gtcaattgta catttcgtgc
    43681 gctttttact ttcagaagaa ccttaagaaa accttaagaa gcattgttta ggtttcgttt
    43741 aagttaatcg accatactgg agatcgtcag aaaatatttc caggagatgg catgattcgc
    43801 ttatctgaac aaagtcccct gggcaccgga agacatcgga aatgttatgc gcatccggaa
    43861 gatgcccaac gctgtatcaa gattgtctac catcgtggcg atggtggcga taaagagatc
    43921 cgccgtgagt taaagtatta cgcgcatctt ggtcgccggt taaaagactg gagtggaata
    43981 ccgcgctatc acggtaccgt tgagacggat tgcggaaccg gttatgtcta cgatgtgatt
    44041 gccgattttg acggcaagcc ttccattacg ctgaccgaat ttgctgaaca atgccgttac
    44101 gaagaagaca ttgcacaact gcgccagtta ctgaaacagc tgaagcgtta tttgcaggat
    44161 aaccgtatcg tgacgatgtc gttgaagccg cagaatatcc tctgtcaccg cattagtgag
    44221 tcagaggtta tccccgtcgt ctgcgacaac attggtgaaa gcacattgat tccactggcg
    44281 acctggtcga agtggtgttg tctgcgtaag caagagcggt tatggaaacg atttattgcc
    44341 caaccggcgc tggctattgc cctgcagaaa gatctgcaac ctcgtgagag caaaacgctg
    44401 gccctcactt cgggcgaagc ttaatccagc tggtgttgtt gcataaacgg ctcaccgcct
    44461 aactgataca tctgccgtaa aatccacgcc tgacggctac gcacgtagcc cgatggcgcg
    44521 gagactttga aacgaagtgg attaggtaat acagctgcca gtaatgcagc ttccgatcgg
    44581 gtaagtttgc tcgcgggttt gtggaaataa cgttgtgccg cagcttcgac gccaaacaca
    44641 ccgtcgccaa attcggcgat attcaggtaa accgtcagga tacgcttttt gctccagacc
    44701 gtttctatcc ccagcgttaa tccggcttcc agcccttttc gaacccagct acgcccatcc
    44761 cataaaaaga gatttttggc cgtctgctga gaaatcgttg atgcaccgcg aatacggttt
    44821 tcattgcgct cgttgtgcgc cagggctttc tcaatggaag cgacatcaaa gccccagtgc
    44881 tcagaaaatt tctgatcttc tgcggcaatc accgccagtc ccatccatgg cgagatttga
    44941 tccatactga cccagtcaga atgtgccacg taacgaaaat tgccatgcag ccaggcgctg
    45001 acctgtcgct cgaccattac cgctgagaag ggaacaggcg caacgctaaa caacgcgatg
    45061 cccccgcccc agaaaacggc gaggacaacc attaaccgca aaagaaaacg gcgaacgaaa
    45121 ctaaacaccg ttaagcggct tttactcatt cagccagaac cagcacgcgg gaaaccagct
    45181 tatcaatgcc gctcgccgct tctgcaatgt tctgcgccag catatatgct ggggtggtga
    45241 caatcttatt atcttcatca accacgatat catcgacagg acacggcaca tgctccgcgc
    45301 ccatctcttc cagcacttct gcggtatcga tatcagtacc gatggtcaaa cgcagcggga
    45361 aatcgaaaat tttcggcagc atcgccgggg caatacacat aaaaccaagc ggttttccgg
    45421 cctgatgcat cgcttgtgcc agcgccttta attcacggtc aacggtgcat tcgctaccaa
    45481 gagtggcaaa attgcttaaa ttcttcgccg cgccaaaccc ccccggcaca atcaacgcat
    45541 ccagttcagc ggcatcggcc tgggccagag gacggatttc accgcgcgtt attcgtgccg
    45601 cttcaatcag cacgttgcgc gtttccgtca tcgcttcgcc agttaaatgg ttgataacat
    45661 caacctgctg cttatccggt gcaaagcaga ccgcctgcgc accgctgcgt gaaatagcta
    45721 acaacgtcaa caccgcttca tgaatttcag aaccgtcata gacgccgcat ccgctcagaa
    45781 ttacgccaat tttcttcatt gtgatcatcc tttcgcaatt gactgaaaca cattaatttt
    45841 tttaataaaa atggtacgca tcacacattt aactgattca tgtaacaaat catttaagtt
    45901 ttgctatctt aactgcgtgc ggcctgaaaa acagtgctgt gcccttgtaa ctcatcataa
    45961 taatttacgg cgcagccaag atttccctgg tgttggcgca gtattcgcgc accccggtct
    46021 agccggggtc attttttagt ggcttttgcc acccacgctt tcagcacttc tacgtcgtga
    46081 cgccactctt ctttcatctc ttcaatccat tcaccgacgt tatcttccca ggcaggaagg
    46141 tccggagact gaatttgctg acccagctgt tgcagatggc gtaaccccac tgaccccgcc
    46201 gcacctttaa ttttatgtcc ttcctctaca atgccttttt tatcctgcgc cgtcagattc
    46261 gactccagca cgctgacata gcccggcatc atcttctcaa acaccgctaa cccgtcggtg
    46321 atcagcttcg gtcctacaag ttcgagatac tgttccagca tgggaatatc gagcaatgct
    46381 tctgatttac tgttctcttc tgtcgtcacc gtactctcct catcatcctg ggtatcccag
    46441 aatttcttga tcatcgcggt tagcgccgga accgaaagcg gcttactcag cacatcatcc
    46501 attccagcat tgaggtactc ttgtttgtct ttcagcacgt tagcggttaa ggccaccagc
    46561 ggcggtaaat cctcgcgcgg ataacgcttc gtcagttcac gagagatatc cagcccggtc
    46621 atatctggca actgaatatc cagcaacacc aggtcgtatt cgcccggttt aaacatctcc
    46681 agcgccgcct tgccggtcat ggcgacatca acgctgttac ctaatttttc cagcacagaa
    46741 cgcgcaacaa tcacgttcag ttcaatgtct tccaccagca gcacattcag cgccggtaaa
    46801 ggcatatcgt cttcatcaaa cgcatcatcg acctcttctg ctaccgacgg tgcgtggatc
    46861 gtcaacgtaa aggttgaacc tttgccctgt tcgctggtaa ccgtaatatc gccgcccata
    46921 tttttcgcca gacgacgaga aacggccaga ccaataccgg tgccggtggc aggtttaccg
    46981 ccatgactgt ctttcacctg gtaatacatg gcgaaaattt tatccagctc atcctgcgga
    47041 atgccgatac cagagtcttc cacttcaaaa tgcagcatat cgccttcatc gtagcgcacg
    47101 cgcacggtaa cctggccttg ctgggtgaat ttgacggcgt tactgatgag gttccacagg
    47161 atctgccgta aacgcgtccc gtcggtaatg acctgatgcg gtaatggcag cgtcggctcc
    47221 aggttaaagc gcaatccttt ttgttgcgcc tgcaatgcgg agagattttc cagatcggca
    47281 aggaagctgg tgaaatcaac cggttgatta tcaagctgga ccttgcgccg ttccatctta
    47341 tccatgtcga taatatcgtt aaagatattc cccagcgtga cggccgaaac atggatggtc
    47401 ttgagatatt tttcctgctc ggcggtgagt tcggtatcca gcagaatacg gctcaggccg
    47461 acgataccgt tcagcggtgt acgcaattcg tgactgatgg tggagataaa cgtcgttttg
    47521 tcgcggctgg cccgttcaag cgcatcctga taccgcttac gctcggtaat gtcgcgacca
    47581 aagcccatca aaccgtgacg tttacccacg cggtcgtagt acggcacttt acggatttca
    47641 aagcaggctt tgcgcccgtc cgggtaatcc agccactgtt cataggtcag tgacacatta
    47701 tgacggaaca ctttttcatc ggtttcaatg acttttgcgg cggcttccgg tgagtaaaca
    47761 tcagcaggtt tcaggtgaac cagttgtttt tcgctttttc cggtcagcag ctccatcgcg
    47821 cggttacagc cggaaaactc tttatcttcg ttacgataaa aaaccaggtc gggtgaagca
    47881 tcaaggaagg aacgtaagaa tgaggattgc tgctcgagct gaatttgtgt ctcttcgcgc
    47941 tctttgattt caattttcag ttggccgaag gtttcctgca gttctgcttc cgctttttca
    48001 cgaacggcga tttcctgatt tagctgggca atattatctt ttaactgaac gttgaggctc
    48061 aaatcgcgct cgcgcatctc ctccagtttt tgcaccagcc gtgacagacg ttgtcgtgac
    48121 tcctccagtt gctcgacgac caccgataga aagtagaccg cccacggcgt aatcagcaaa
    48181 ccaaagaaga tagaacgaat aacatcaatg ctttcgacct gaccatgcag caccatggtt
    48241 accgccattt gtaccacaat ggcaagaacg acgagggcca gcgccagcaa cattgagaag
    48301 cgcaccagac ctaacttcat catcaggtca acataatact gcgccagcag acgaatttgc
    48361 ttcattaggg aattccttca cgacaacctg ccccaataat acccaattct gacagttacg
    48421 ttataaattg tgtgagaaat gcggaattcc cctcaacgac ctactccgtt ggaggtatcc
    48481 acggacgttc cagcgccgat ccctgtacgc catgttcatt cagatagcga tccagctcga
    48541 ccattcccgt ccatcgattt tcgcaccaca gaggagcaag cagcgtcggg cgacgggcgc
    48601 tggcggaaat gcggtggtag atcacttccg gtggcgtatg gcgaatcatt tctccggcag
    48661 tgagcgtgta atcctccagt tcaataccgt tcaaacgccc cgcttcccag gctttcgcca
    48721 taatgctgcc tttcacaata tgcagcggat gcagctttat gccgtccacg ccggtttcaa
    48781 ccactcgctc cagcgtttgc aaacattcag cctgcccttc gccaggcagg ccgacaatta
    48841 agtgggaaca caccttcagc ccgcgctgac gtgccagctg ggttgtacgc tgataacagg
    48901 caaaatcatg gccgcggttg atgcgatgca gtgttttgtc gtgggcggtt tgtagcccca
    48961 gctccagcca cacttcgtaa ccctggtcct tatattcgca aagcagatcc agcaccgcat
    49021 ccggcacgca gtccgggcgg gtaccaacac acaaaccgac aatattggcc tggctcaccg
    49081 cctgctgata catagaacgc agcacctgaa cttccgcaaa ggtgctggta tacgcctgaa
    49141 agtaggccag atagcgttta gcgcggttaa ctaaattcgc ctggtgcgcc agttgctcgg
    49201 caatggaacg atgctgctgc gcttcatcgg caaacgaggc aacattacag aatgtgcagc
    49261 caccacgccc gatggtaccg tcacggttag ggcagctaaa accgccgtgt agcgtcagct
    49321 tatgcacctt ttgcccataa cgacgggtga gatcaccacc aaacatattg actaatttct
    49381 gtaactgcat aatctgatag accgcgcctt gaaaagaggc caaagcctgc catttttagc
    49441 tcaattcggc gatgacctgg atcaatcgtc ccggcctgct tttatcaact gcataatcaa
    49501 tcaaaattac cgaaatttca tgcataatca cataaatcac ttttgtttat cttgtgtcag
    49561 atttttttat ctccttatgg attttaggca aaaacagtag catgaaacgt cattaccaat
    49621 taaggcagta taaaatgctg gttttctcgt cagttcaa
//
LOCUS       DAZFQD010000016        48726 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.016, whole genome shotgun sequence.
ACCESSION   DAZFQD010000016 DAZFQD010000000
VERSION     DAZFQD010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 48726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48726
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.016"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            378..1595
                     /gene="alaA"
                     /locus_tag="ACHKTW_001049"
     CDS             378..1595
                     /gene="alaA"
                     /locus_tag="ACHKTW_001049"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HFO6462113.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            1679..2278
                     /gene="yfbR"
                     /locus_tag="ACHKTW_001050"
     CDS             1679..2278
                     /gene="yfbR"
                     /locus_tag="ACHKTW_001050"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002953 - 5'-deoxynucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HFO6462114.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNSQIAQEYKAIEKIAQQ
                     KLVDMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(2337..4169)
                     /locus_tag="ACHKTW_001051"
     CDS             complement(2337..4169)
                     /locus_tag="ACHKTW_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HFO6462115.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTGVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTDEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(4256..4906)
                     /gene="hxpA"
                     /locus_tag="ACHKTW_001052"
     CDS             complement(4256..4906)
                     /gene="hxpA"
                     /locus_tag="ACHKTW_001052"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
                     /protein_id="HFO6462116.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTRLEHIEATETEGITALPGAIALLNHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ"
     gene            complement(4917..5411)
                     /locus_tag="ACHKTW_001053"
     CDS             complement(4917..5411)
                     /locus_tag="ACHKTW_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HFO6462117.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALHVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(5493..5948)
                     /gene="yfbV"
                     /locus_tag="ACHKTW_001054"
     CDS             complement(5493..5948)
                     /gene="yfbV"
                     /locus_tag="ACHKTW_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminus macrodomain insulation protein YfbV"
                     /protein_id="HFO6462118.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGQLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            6286..7488
                     /gene="ackA"
                     /locus_tag="ACHKTW_001055"
     CDS             6286..7488
                     /gene="ackA"
                     /locus_tag="ACHKTW_001055"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HFO6462119.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVVFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            7563..9707
                     /gene="pta"
                     /locus_tag="ACHKTW_001056"
     CDS             7563..9707
                     /gene="pta"
                     /locus_tag="ACHKTW_001056"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFO6462120.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIESLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            9897..11417
                     /gene="yfcC"
                     /locus_tag="ACHKTW_001057"
     CDS             9897..11417
                     /gene="yfcC"
                     /locus_tag="ACHKTW_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HFO6462121.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATMIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGDASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(11450..11992)
                     /gene="yfcD"
                     /locus_tag="ACHKTW_001058"
     CDS             complement(11450..11992)
                     /gene="yfcD"
                     /locus_tag="ACHKTW_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HFO6462122.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSEVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(12050..12601)
                     /gene="yfcE"
                     /locus_tag="ACHKTW_001059"
     CDS             complement(12050..12601)
                     /gene="yfcE"
                     /locus_tag="ACHKTW_001059"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HFO6462123.1"
                     /translation="MKLMFASDIHGSLPATERVLELFAQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMLLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPASYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(12657..13301)
                     /gene="yfcF"
                     /locus_tag="ACHKTW_001060"
     CDS             complement(12657..13301)
                     /gene="yfcF"
                     /locus_tag="ACHKTW_001060"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HFO6462124.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLVLGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            13437..14084
                     /gene="yfcG"
                     /locus_tag="ACHKTW_001061"
     CDS             13437..14084
                     /gene="yfcG"
                     /locus_tag="ACHKTW_001061"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HFO6462125.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAELDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRQRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            14141..14503
                     /gene="folX"
                     /locus_tag="ACHKTW_001062"
     CDS             14141..14503
                     /gene="folX"
                     /locus_tag="ACHKTW_001062"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HFO6462126.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            14524..15417
                     /locus_tag="ACHKTW_001063"
     CDS             14524..15417
                     /locus_tag="ACHKTW_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HFO6462127.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVVTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHEQKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNELRG
                     PFNMVSPYPIRNEQFAHALGHALHRPAILRVPATAIRLLMGESSVLVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(15465..16355)
                     /gene="rpnB"
                     /locus_tag="ACHKTW_001064"
     CDS             complement(15465..16355)
                     /gene="rpnB"
                     /locus_tag="ACHKTW_001064"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HFO6462128.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARDFIDIHLPAPLRKLCDLTT
                     LKLEPNSFIDEDLRQYYSDLLWSVKTQEGVGYIYVVIEHQSKPEELMAFRMMRYSIAA
                     MQNHLDAGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDIT
                     VVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQ
                     TGDAQRFRAFIGEITERAPQEKEKLMTIADRLREEGAMQGKHEEALRIAQEMLDRGLD
                     RELVLMVTRLSPDDLIAQSH"
     gene            complement(16552..17325)
                     /gene="hisP"
                     /locus_tag="ACHKTW_001065"
     CDS             complement(16552..17325)
                     /gene="hisP"
                     /locus_tag="ACHKTW_001065"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HFO6462129.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(17333..18049)
                     /locus_tag="ACHKTW_001066"
     CDS             complement(17333..18049)
                     /locus_tag="ACHKTW_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO6462130.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(18046..18732)
                     /gene="hisQ"
                     /locus_tag="ACHKTW_001067"
     CDS             complement(18046..18732)
                     /gene="hisQ"
                     /locus_tag="ACHKTW_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HFO6462131.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNTVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(18822..19604)
                     /gene="hisJ"
                     /locus_tag="ACHKTW_001068"
     CDS             complement(18822..19604)
                     /gene="hisJ"
                     /locus_tag="ACHKTW_001068"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HFO6462132.1"
                     /translation="MKKLVLSLSLVLAFSSATAAFAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIDLAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(19825..20607)
                     /gene="argT"
                     /locus_tag="ACHKTW_001069"
     CDS             complement(19825..20607)
                     /gene="argT"
                     /locus_tag="ACHKTW_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HFO6462133.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYAPFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGSSVKDKKYFGDGTG
                     VGLRKDDAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(20873..21442)
                     /gene="ubiX"
                     /locus_tag="ACHKTW_001070"
     CDS             complement(20873..21442)
                     /gene="ubiX"
                     /locus_tag="ACHKTW_001070"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HFO6462134.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTHDARDIAASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            complement(21537..23054)
                     /gene="purF"
                     /locus_tag="ACHKTW_001071"
     CDS             complement(21537..23054)
                     /gene="purF"
                     /locus_tag="ACHKTW_001071"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFO6462135.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIDAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(23091..23579)
                     /gene="cvpA"
                     /locus_tag="ACHKTW_001072"
     CDS             complement(23091..23579)
                     /gene="cvpA"
                     /locus_tag="ACHKTW_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HFO6462136.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIRGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(23838..24500)
                     /gene="dedD"
                     /locus_tag="ACHKTW_001073"
     CDS             complement(23838..24500)
                     /gene="dedD"
                     /locus_tag="ACHKTW_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HFO6462137.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVAPPKPKPVEPPKPKVEAPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(24490..25758)
                     /gene="folC"
                     /locus_tag="ACHKTW_001074"
     CDS             complement(24490..25758)
                     /gene="folC"
                     /locus_tag="ACHKTW_001074"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HFO6462138.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGREKAGIFRSEKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWTFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(25828..26742)
                     /gene="accD"
                     /locus_tag="ACHKTW_001075"
     CDS             complement(25828..26742)
                     /gene="accD"
                     /locus_tag="ACHKTW_001075"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFO6462139.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDVLKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQALEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPGFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(26898..27557)
                     /locus_tag="ACHKTW_001076"
     CDS             complement(26898..27557)
                     /locus_tag="ACHKTW_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFO6462140.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VTPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGDKLFS
                     NPDSKIFRRSYLDKTHQFYERHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLIFTYAGYFFGTLPFIQSNLKLMIVGIIFVSILPGVFEFIRHKRAAARAA
                     K"
     gene            complement(27640..28452)
                     /gene="truA"
                     /locus_tag="ACHKTW_001077"
     CDS             complement(27640..28452)
                     /gene="truA"
                     /locus_tag="ACHKTW_001077"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFO6462141.1"
                     /translation="MSDQQQLPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(28452..29465)
                     /locus_tag="ACHKTW_001078"
     CDS             complement(28452..29465)
                     /locus_tag="ACHKTW_001078"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFO6462142.1"
                     /translation="MSEGWNIAVLGATGAVGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(29531..30667)
                     /gene="pdxB"
                     /locus_tag="ACHKTW_001079"
     CDS             complement(29531..30667)
                     /gene="pdxB"
                     /locus_tag="ACHKTW_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033711 - 4-phosphoerythronate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HFO6462143.1"
                     /translation="MKILVDENMPYARDLFSRLGEVTAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHDRTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQHADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            30766..31761
                     /gene="flk"
                     /locus_tag="ACHKTW_001080"
     CDS             30766..31761
                     /gene="flk"
                     /locus_tag="ACHKTW_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0010468 - regulation of gene expression
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HFO6462144.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNNRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMTLKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRVMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            complement(31758..32936)
                     /locus_tag="ACHKTW_001081"
     CDS             complement(31758..32936)
                     /locus_tag="ACHKTW_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO6462145.1"
                     /translation="MTAVSQTETRSSANFSLFRIAFAVFLTYMTVGLPLPVIPLFVHH
                     ELGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVVGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALAITTMVLPVLAWACNGTVRKVPALAGERPSLWSVVGLI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(33201..34421)
                     /gene="fabB"
                     /locus_tag="ACHKTW_001082"
     CDS             complement(33201..34421)
                     /gene="fabB"
                     /locus_tag="ACHKTW_001082"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HFO6462146.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNVKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAHIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMKMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKD"
     gene            34580..36586
                     /gene="mnmC"
                     /locus_tag="ACHKTW_001083"
     CDS             34580..36586
                     /gene="mnmC"
                     /locus_tag="ACHKTW_001083"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HFO6462147.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEVRFPEHPHPLFVVAESGFGTGLNFLTLWQAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLDAGRVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQDAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSAPWFNRTGSSKREAAIIGGGIAS
                     ALLSLALLRRGWQVTLYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSTLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDEAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLAALNPNRLWVRKLLKGKAVKAG"
     gene            complement(36707..36985)
                     /locus_tag="ACHKTW_001084"
     CDS             complement(36707..36985)
                     /locus_tag="ACHKTW_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HFO6462148.1"
                     /translation="MIAEFESRILALIDDMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQQ"
     gene            complement(37019..37567)
                     /gene="epmC"
                     /locus_tag="ACHKTW_001085"
     CDS             complement(37019..37567)
                     /gene="epmC"
                     /locus_tag="ACHKTW_001085"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HFO6462149.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     AFDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLANGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            complement(37567..38376)
                     /locus_tag="ACHKTW_001086"
     CDS             complement(37567..38376)
                     /locus_tag="ACHKTW_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFO6462150.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVTLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLFDSHGQEILHWLGMN"
     gene            complement(38376..39200)
                     /gene="mepA"
                     /locus_tag="ACHKTW_001087"
     CDS             complement(38376..39200)
                     /gene="mepA"
                     /locus_tag="ACHKTW_001087"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HFO6462151.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVSTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPPGDGCGAELQSWFAPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(39204..40289)
                     /gene="aroC"
                     /locus_tag="ACHKTW_001088"
     CDS             complement(39204..40289)
                     /gene="aroC"
                     /locus_tag="ACHKTW_001088"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFO6462152.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSLVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     LGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            complement(40324..41256)
                     /gene="prmB"
                     /locus_tag="ACHKTW_001089"
     CDS             complement(40324..41256)
                     /gene="prmB"
                     /locus_tag="ACHKTW_001089"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFO6462153.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLLAAREYFAIYKD"
     gene            41422..41973
                     /gene="smrB"
                     /locus_tag="ACHKTW_001090"
     CDS             41422..41973
                     /gene="smrB"
                     /locus_tag="ACHKTW_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HFO6462154.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            complement(42046..42900)
                     /locus_tag="ACHKTW_001091"
     CDS             complement(42046..42900)
                     /locus_tag="ACHKTW_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="StfH/YfcO family fimbrial adhesin"
                     /protein_id="HFO6462155.1"
                     /translation="MKILRHVLCLLLLLMFNTCGQAATKVGELYVPAGASSPATEFEF
                     HLIIATPDSVNYAIFKATFMPNSQPDLVSWTGDSSTQPSMSKISDSRVSMSACPGLEQ
                     YDSQTKTGWTCNELKMFVYYDGNLHGCPWIVSSFVKSRDPFAKTYDDDFPDYIGPTKV
                     SSSCPAVPLAPYDVSWNENYVVHNKVVRLQSTGGVIEQTLPTFLMENGKLCNGNNFDE
                     RGAYCRFIAQQMTFSTSGCDNAKVTVTPEPQPITSRQLHDMKLRVDTTSRQPIDSTCR
                     FTYILNMY"
     gene            complement(42902..43441)
                     /locus_tag="ACHKTW_001092"
     CDS             complement(42902..43441)
                     /locus_tag="ACHKTW_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO6462156.1"
                     /translation="MKTAHIYSPGYALLAGLLLTASTGAQAVDNNLHFYGNLLSKSCT
                     LVVDGANLAEIHFPTVSNRDLMVSGQSAHLPVVFKLKDCKGPAGYNVKVTLSGTEDSG
                     QPGFLAVDDTSTAQGVGIGMETLDGKRVDINNTTGATFALTNGNNDINFRAWIQAKSG
                     RDVTIGDFTASLTATFEYI"
     gene            complement(43438..43926)
                     /locus_tag="ACHKTW_001093"
     CDS             complement(43438..43926)
                     /locus_tag="ACHKTW_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001709066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO6462157.1"
                     /translation="MKKFLLLLLCADCGAVLAAEDDITFHGTLMSPPACSISGGQTIE
                     VQFRDLIIDSIDGNYGRQKVDYELSCDSDVRDPEWNMTLSWTGTATSYNDSAIETDVP
                     GFGIELQHDGQRFKLNEPLSINATDFSQKPKLEAVPVKAADAVLTDTTFSAYATLRVD
                     YQ"
     gene            complement(43923..44432)
                     /locus_tag="ACHKTW_001094"
     CDS             complement(43923..44432)
                     /locus_tag="ACHKTW_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001733155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO6462158.1"
                     /translation="MSHKFVAGAGLLVLAGWGISGLAVAGSDIKQVDLTLRILVDGPP
                     PCSVKGSAVKFGNVIINRIDGTNYQQDAKYTLNCGNKVSDDLRMQLKGNTAVINGETV
                     LSTDIAGLGVRIENAADNSLFAVGENNWTPFNVNTQPQLKAVPVKQSGVQLTPSEFNA
                     TMTMVVDYQ"
     gene            complement(44448..45200)
                     /locus_tag="ACHKTW_001095"
     CDS             complement(44448..45200)
                     /locus_tag="ACHKTW_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000482750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFO6462159.1"
                     /translation="MFNLTNTAKIVVPALALLATAVSFSSHASVTPDRTRLVFNESDK
                     SISVTLRNNDPTLPYLAQSWIEDAKGNKISSPLAVLPPVQRIDAMMNGQVKVQGLPDI
                     NKLPADRESVFYFNVREIPPKSNKPNTLQIALQTRIKLFWRPKALENTSMKSPWQHKV
                     TLIRNGQDFTVNNPTPYYVIISNASAKKGGNPPAGFTPLMMEPKTSAPLNAKMDSVPV
                     LTYINDYGARMPLYFICTGNSCQVDEEQSRKG"
     gene            complement(45220..47865)
                     /gene="yfcU"
                     /locus_tag="ACHKTW_001096"
     CDS             complement(45220..47865)
                     /gene="yfcU"
                     /locus_tag="ACHKTW_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher protein YfcU"
                     /protein_id="HFO6462160.1"
                     /translation="MPDHSLFRLRILPWCIALAMSGSYSSVWAEDDIQFDSRFLELKG
                     DTKIDLKRFSSQGYVEPGKYNLQVQLNKQPLAEEYDIYWYAGEDDASKSYACLTPELV
                     AQFGLKEDVAKNLQWSHDAKCLKSGQLEGMEIKADLSQSALVISLPQAYLEYTYPDWD
                     PPSRWDDGISGIVADYSINAQTRHEENGGDDSNEISGNGTVGVNLGPWRMRADWQTNY
                     QHTRSNDDDDEFSGDETQKKWEWSRYYAWRALPSLKAKLALGEDYLKSDIFDGFNYVG
                     GSVSTDDQMLPPNLRGYAPDISGVAHTTAKVTVSQMGRVIYETQVPAGPFRIQDLGDS
                     VSGTLHIRIEEQNGQVQEYDISTASMPYLTRPGQVRYKIMMGRPQEWGHHVEGEFFSG
                     AEASWGIANGWSLYGGALGDENYQSAALGVGRDLSTFGAVAFDVTHSHTKLDKDTAYG
                     KGSLDGNSFRVSYSKDFDQLNSRVTFAGYRFSEENFMTMSEYLDASDSGMVRTGNDKE
                     MYTATYNQNFRDAGVSVYLNYTRHTYWDREEQTNYNIMLSHYFNMGSIRNVSISMTGY
                     RYEYDNQADKGMYISLSMPWGDNSTVSYNGNYGSGTDSSQVGYFSRVDDATHYQLNVG
                     TSDKHTSVDGYYSHDGSLAQVDLSANYHEGQYTSAGLSLQGGATLTAHGGALHRTQNM
                     GGTRLLIDADGVADVPVEGNGAAVYTNMFGKAVVSDVNNYYRNQAYIDLNRLPENAEA
                     TQSVVQATLTEGAIGYRKFAVISGQKAMAVLRLQDGSHPPFGAEVKNDNEQTVGLVDD
                     DGNVYLAGVKPGEHMSVFWSGVAHCDINLPDPLPADLFNGLLLPCQHKGNVAPITSPA
                     VKPAIQEQTQRVTPTEPPTSISVNQ"
     gene            complement(47947..48510)
                     /locus_tag="ACHKTW_001097"
     CDS             complement(47947..48510)
                     /locus_tag="ACHKTW_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO6462161.1"
                     /translation="MSKFVKTAIAAAMVMGVFTSTATIAAGNNGTARFYGTIEDSVCS
                     IVPDDHKLEVDMGDIGAEKLKNNGTTTPKSFQIRLQDCVFDTQETMTTTFTGTVSSAN
                     SGNYYTIFNTDTGAAFNNVSLAIGDSLGTSYKSGMGIDQKIVKDTSTNKGKAKQTLNF
                     KAWLVGAADAPDLGNFEANTTFQITYL"
BASE COUNT        11937 a        13163 c        12156 g        11470 t
ORIGIN      
        1 taacaatagt ttcacacctt gcccccatta cgacacctcg gaaatcatca cgctgttaaa
       61 ctctccgtaa tgcggaaata tcatcatcgc gtttcattag tgaattctta aatgaggcat
      121 tttcacacaa ttatcttaca gataaaaaaa ccagacttac aattaagaat cagaacaatt
      181 cactatataa cattgcatgt aaagcatata cacctcatta ttttgtcatt attaagttat
      241 taacagcaca atcgagcctt cccctctggc aaaatcttat tctgcagacc ttcaaaacac
      301 cgtcctgggg gagtacattg ttctaagctg acttccacgg cagggagtgg cgataacagc
      361 aaaaaaggtc aagattcatg tcccccattg aaaaatccag caaattagag aatgtctgtt
      421 atgacatccg tggtccggtg ctgaaagaag caaaacgcct ggaagaagaa ggtaacaagg
      481 tactgaaact gaacatcggc aacccagccc cgttcggttt tgacgcgcca gatgaaatcc
      541 tcgttgacgt gatacgcaac ctgcctaccg ctcaagggta ttgcgattcc aaaggtcttt
      601 actccgcgcg taaagccatc atgcagcact accaggctcg tggcatgcgt gatgttaccg
      661 tggaagatat ttacatcggc aatggtgtat cggagcttat cgttcaggca atgcaggcat
      721 tgctgaacag cggggacgaa atgttggttc ctgcaccaga ttacccacta tggaccgcgg
      781 cggtttcgct ttccagcggt aaagcggtgc attatctttg cgatgaatcc tctgactggt
      841 tcccggacct cgatgatatt cgcgctaaaa ttacgcctcg tacgcgtggg atcgttataa
      901 tcaacccaaa taacccaacc ggcgcggtat attccaaaga gcttttaatg gagattgtgg
      961 agattgcacg tcagcataat ctcattatct tcgccgatga aatttatgac aaaattcttt
     1021 acgacgacgc tgagcatcac tcaattgcgc cgctggcacc tgacctgctg accattacct
     1081 ttaacggact gtcgaaaacg taccgcgttg caggcttccg tcaggggtgg atggtgttga
     1141 acgggccgaa aaaacacgcc aaaggctaca tcgaaggtct ggaaatgctg gcttcaatgc
     1201 gcctgtgtgc taacgttcct gcgcaacacg ccattcagac cgcgctgggt ggttatcaga
     1261 gcatcagtga atttattacc cctggcggtc gtctttatga gcagcgtaac cgcgcgtggg
     1321 aactgatcaa cgatattccg ggcgtttcct gcgtgaaacc tcgtggtgcg ctgtatatgt
     1381 tcccgaaaat cgacgccaaa cgctttaaca ttcacgacga tcagaaaatg gtgctggatt
     1441 tcctgttgca ggaaaaagtt ctgttggtgc aagggacggc attcaactgg ccgtggccgg
     1501 atcacttccg cattgtcacg ctaccgcgtg tcgatgatat cgagctgtct ttgagcaagt
     1561 tcgcgcgttt cctttctggt tatcatcagc tgtaatctta atttcactgc cggagattgc
     1621 atccggcagc gttatcccgc cacaatgacc tgatgatgtc atcatacgta aggtcactat
     1681 gaaacagagc catttttttg cccatctctc ccgcctgaaa ctcattaacc gctggccgct
     1741 catgcgcaac gtgcggacgg aaaatgtgtc cgaacacagt ttgcaggtag cgatggtcgc
     1801 ccatgcgctg gcagctatca aaaatcggaa atttggcggt aatgtcaacg ccgaacgtat
     1861 cgctttactg gcgatgtacc acgatgcctc agaagtgctc accggcgatc tccctactcc
     1921 ggtgaaatac ttcaattcgc aaatcgctca ggagtacaag gctattgaaa aaatcgctca
     1981 gcaaaaactg gtcgatatgg ttccggaaga gctgcgggat atctttgcgc cgttaattga
     2041 cgagcatgca tatagcgatg aagaaaaatc gctggtgaaa caggcagatg cactgtgtgc
     2101 atatctgaaa tgtctggaag aactcgcggc cggaaataat gaattcttgc tggcaaaaac
     2161 gcgactggaa gcgacgcttg aagcgcgtcg cagccaggag atggactact tcatggaagt
     2221 atttgttccc agcttccatc tttcgctcga tgagattagc caggattcac cgctgtaagc
     2281 agccggagtc tgcgtcgcat caggcaataa gcgccggatg cgacatcagg ctcttgtcaa
     2341 aacggaaaca gcatcgggat catcaccaca caaaccgcca tcacgataat ggtgaacggt
     2401 acccccaact tcacaaagtc actgaagctg taatttcccg gacctaaaac cagtgtgtta
     2461 acaggtgaag aaaccggtgt cataaaggcg gcggatgctg ccatcgccac gaccatcgcg
     2521 aatggataag gcgacacccc catcgttttg gcagcagcca gcgcaatcgg agccatcaac
     2581 accgccgtcg cggtattaga gataaatagc ccaataaccg ccgacaagac aaacaaacac
     2641 cccagcatca tatgtggccc gtaaccaccg ccaatgtcca tcagcccttt caccgccagc
     2701 gcgacacctc ccgttttctg taatgccaca gcaaacggca tcatcccaac gatcaaaata
     2761 atgctcggcc agtgaatgga tttataggcg ctttcagcat ctatacagcg gaatttcccc
     2821 atcagcaggc aggcgatgat agcggcaaca ggattaggaa tttcatctgt cagcattaac
     2881 gccaccatta gcaccagaca gaaaatggca tggggtgcct ggctgtgcgc gggcgatgct
     2941 tcactcacct cttccggtaa gttcagcgct acgaagtcgc ggccctgttt ggccagcata
     3001 ccgatcagtt tccagttacc cacaaccagg atgatatcgc ccagcagcag aggctcatcc
     3061 gccagcgaac cttccagcgc cacgccattg cgctttagcc ccaccacatt cagtccgtag
     3121 cgggtacgaa aaccaatttc gcgcaccgat ttaccaatca gttctgactc aggaattaat
     3181 gaaatctctg ccatgcccac atcaagggcc tggtcagaaa aatactcgcc gcgcagtacc
     3241 atcggctcca gcaattgctc actacaaaat tgccggagat cgacatcagc cgcagacata
     3301 tcaataagca aaacgtcacg cgcgcgaaat tcagaaaccc cattaacgtt cacgataacg
     3361 cgacgaaaac gccgccagcg ttcaacaccg atgacgttag cgccataacg ctcacgtaat
     3421 ttgagatcat ccagccgttg acctatcatt ggcgatccgg ggcgaatagc cagacgtcgc
     3481 gcacgcccgg tcagtcgata ttcacggata agatcgcgaa aggttcgacg cgtccagcct
     3541 tcgcgctgcg gggtctgggt atcccctttc agcatgaaac gcatcactaa catatacaaa
     3601 atacccagca ccagcacgac caggccaata ggtgttacgc taaagaaact gaagccgtga
     3661 tagccttcac gcagcaattc actgttgact accaggttcg gcggcgtcgc caccagcgtc
     3721 atcatgccgc taatcagccc ggcaaaactt aacggcatca tcagacgtga cggcgaagtt
     3781 tgcatacgca tggcaacgct taacaccacg ggaataaaga tagcgacaac gccggttgaa
     3841 ctcataaacg cgccaagccc cgcgacggtc agcatcaaca aaaccaacat tttgatttca
     3901 ctgttgcccg ccactttgac cagccatgtt cccattacgg tggcaacacc ggtacggacc
     3961 aaaccatcgc caataataaa caaggcggca atcaggacaa cgttaggatc agaaaagccg
     4021 gaaaatactt cggggaccgt cagcgttccg cttaatgcaa acgcgacaat aacaaacaaa
     4081 gcgaccgcat ccatacgcac tctgcccgtc gcaaacaaga cgatggcaac ggccagtaat
     4141 gaaagaaccc aaatcaattc accgttcaca acttatcctt gttaattgag ggggatgact
     4201 tgattctgcc ataaaaaagc cccgacgaga cggggctaaa tcatgatcag gtgtttcact
     4261 gaataataac atcgccattt ggctgtttgg tcacagtaat ttgctccaga ctgtggagga
     4321 ccaaatcgac ctcattcagg cgcggggtat ctgccggagc gttaaccgca atgacatggc
     4381 aacccgccgc caggccagaa agcacgccag cgggagcatc ttccaccacc acacactcct
     4441 gcggcgcaag ccccagcagc tgcgcaccta acagatacgc atcaggttct ggttttccgc
     4501 gcttcactcg ctcagcggtt acaaacacct ctggtgcggg aagcccagct attttatggc
     4561 gcgctcgcgc taccggcatg gagccagaag tcacaatggc ccacggaata cctgctttat
     4621 tcaaatgatt gagtaaggcg attgcccccg gaagcgcggt aataccttcg gtttccgtgg
     4681 cctcgatgtg ctccagacgc gtaaactcgg cggcaatatc agcctcggat ttgcccgcca
     4741 taaaatggcg caaagaggtg atcgcctgtt taccgtgaat gaaagccagc acctcttccg
     4801 gcgctaaccc atgacgtctg gcccagttgc tccacgcccg ttctaccgca ggcagggaat
     4861 ccaccagcgt tccatcaaga tcaaacagaa aacctttgca ccgcacgcgg gcctcctcag
     4921 gcgttgataa tttgattaat ttcgttggcg ctcaaatggt actgacgcgg gcaggcatgc
     4981 cacacattaa gcatgcgctg atatttttcc cacattggcg tctgggcgtt aaaaccgtga
     5041 gttccggcgt caaaatgggt atagcgccct tccacattaa ccataaagcg gacataacca
     5101 aggtaacgtg cttcagtggc ggcgtcaaag ccgaggaagg tgacacgacg ttcatcgatg
     5161 gattgctggt cctgcaaatt agaccaggaa acatgcaacg catgatacat ctccataatg
     5221 tcgatgatag tgcggcaggt ttcttctttc agctcgccaa actcgcgatc caattcacgc
     5281 atctgtaatc cgtaaccacg ctcaataatt gtttgcaggc gacggtaacg ttcagcattt
     5341 gccggatcga gcatagtcat catcttgtac tgattagaca aaataagacg ttgcgcgttg
     5401 gtcatttcca ttgttgactc ctgtatcact ctactacggt gaaaaaaaga aggctgagta
     5461 tgccttcttt tatatgcgta atcaggggtc aattacaaat catcaaggaa agttttatcc
     5521 agttgtttga aggcgcgctt aagcgtgtca gctaatgcct ggtaatcagg cttgccttca
     5581 acgggtgcca acacctgtcc agactcctgc aatttaccgc gaacttcata aaaccagttg
     5641 aggatagcag ggggtaatgg cgttacagaa cgcttgccca gccaccataa tccctgcatg
     5701 ggtaaactta aggcgaacag cgcagtggca actgccggcc caagctgacc gcccagggca
     5761 atctgccagc agagagtaaa tacggcgatc ggcggcataa aacggatcgc ataacgcgtc
     5821 atcttgataa cgcgattttc gacaaagacc ggggcaaggc gtttttccag cggccacgtc
     5881 tttgagtaat gctgtccccg gcgaaacaag ctaaaaaaat taacagaacg attatccggc
     5941 gttgacatgc ttcacctcaa cttcacatat aaagattcaa aaatttgtgc aaattcacaa
     6001 ctcagcggga caacgttcaa aacattttgt cttccatacc cactatcagg tatcctttag
     6061 cagcctgaag gcctaagtag tacatattca ttgagtcgtc aaattcatat acattatgcc
     6121 attggctgaa aattacgcaa aatggcatag actcaagata tttcttccat catgcaaaaa
     6181 aaatttgcag tgcatgatgt taatcataaa tgtcggtgtc atcatgcgct acgctctatg
     6241 gctccctgac gtttttttag ccacgtatca attataggta cttccatgtc gagtaagtta
     6301 gtactggttc tgaactgcgg tagttcttca ctgaaatttg ccatcatcga tgcagtaaat
     6361 ggtgaagagt acctttctgg tttagccgaa tgtttccacc tgcctgaagc acgtatcaaa
     6421 tggaaaatgg acggcaataa acaggaagcg gctttaggtg caggcgccgc tcacagcgaa
     6481 gcgctcaact ttatcgttaa tactattctg gcacaaaaac cagaactgtc tgcgcagctg
     6541 actgctatcg gtcaccgtat cgtacacggc ggcgaaaagt ataccagctc cgtagtgatc
     6601 gatgagtctg ttattcaggg tatcaaagat gcagcttctt ttgcaccgct gcacaacccg
     6661 gctcacctga tcggtatcga agaagctctg aaatctttcc cacagctgaa agacaaaaac
     6721 gttgctgtat ttgacaccgc gttccaccag actatgccgg aagagtctta cctctacgcc
     6781 ctgccttaca acctgtacaa agagcacggc atccgtcgtt acggcgcgca cggcaccagc
     6841 cacttctatg taacccagga agcggcaaaa atgctgaaca aaccggtaga agaactgaac
     6901 atcatcacct gccacctggg caacggtggt tccgtttctg ctatccgcaa cggtaaatgc
     6961 gttgacacct ctatgggcct gaccccgctg gaaggtctgg tcatgggtac ccgttctggt
     7021 gatatcgatc cggcgatcat cttccacctg cacgacaccc tgggcatgag cgttgacgca
     7081 atcaacaaac tgctgaccaa agagtctggc ctgctgggtc tgaccgaagt gaccagcgac
     7141 tgccgctatg ttgaagacaa ctacgcgacg aaagaagacg cgaagcgcgc aatggacgtt
     7201 tactgccacc gcctggcgaa atacatcggt gcctacactg cgctgatgga tggtcgtctg
     7261 gacgctgttg tattcactgg tggtatcggt gaaaatgccg cgatggttcg tgaactgtct
     7321 ctgggcaaac tgggcgtgct gggctttgaa gttgatcatg aacgcaacct ggctgcacgt
     7381 ttcggcaaat ctggtttcat caacaaagaa ggtacccgtc ctgcggtggt tatcccaacc
     7441 aacgaagaac tggttatcgc gcaagacgcg agccgcctga ctgcctgatt tcacaccgcc
     7501 agctcagctg gcggtgctgt tttgtaaccc gccaaatcgg cggtaacgaa agaggataaa
     7561 ccgtgtcccg tattattatg ctgatcccta ccggaaccag cgtcggtctg accagcgtca
     7621 gccttggcgt gatccgtgca atggaacgca aaggcgttcg tctgagcgtt ttcaaaccta
     7681 tcgctcagcc gcgtaccggt ggcgatgcgc ccgatcagac tacgactatc gtgcgtgcga
     7741 actcttccac cacgacggcc gctgaaccgc tgaaaatgag ctacgttgaa ggtctgcttt
     7801 ccagcaatca gaaagatgtg ctgatggaag agatcgtcgc aaactaccac gctaacacca
     7861 aagacgctga agtcgttctg gttgaaggtc tggtcccgac acgtaagcac cagtttgccc
     7921 agtctctgaa ctacgaaatc gctaaaacgc tgaatgcgga aatcgtcttc gttatgtctc
     7981 agggcactga caccccggaa cagctgaaag agcgtatcga actgacccgc aacagcttcg
     8041 gcggtgccaa aaacaccaac atcaccggcg ttatcgttaa caaactgaac gcaccggttg
     8101 atgaacaggg tcgtactcgc ccggatctgt ccgagatttt cgacgactct tccaaagcta
     8161 aagtaaacaa tgttgatccg gcgaagctgc aagaatccag cccgctgccg gttctcggcg
     8221 ctgtgccgtg gagctttgac ctgatcgcga ctcgtgcgat cgatatggct cgccacctga
     8281 atgcgaccat catcaacgaa ggcgacatca atactcgccg cgttaaatcc gtcactttct
     8341 gcgcacgcag cattccgcac atgctggagc acttccgtgc cggttctctg ctggtgactt
     8401 ccgcagaccg tcctgacgtg ctggtggccg cttgcctggc agccatgaac ggcgtagaaa
     8461 tcggtgccct gctgctgact ggcggctacg aaatggacgc gcgcatttct aaactgtgcg
     8521 aacgtgcttt cgctaccggc ctgccggtat ttatggtgaa caccaacacc tggcagacct
     8581 ctctgagcct gcagagcttc aacctggaag ttccggttga cgatcacgaa cgtatcgaga
     8641 aagttcagga atacgttgct aactacatca acgctgactg gatcgaatct ctgactgcca
     8701 cttctgagcg cagccgtcgt ctgtctccgc ctgcgttccg ttatcagctg actgaacttg
     8761 cgcgcaaagc gggcaaacgt atcgtactgc cggaaggtga cgaaccgcgt accgttaaag
     8821 cagccgctat ctgtgctgaa cgtggtatcg caacttgcgt actgctgggt aatccggcag
     8881 agatcaaccg tgttgcagcg tctcagggtg tagaactggg tgcagggatt gaaatcgttg
     8941 atccagaagt ggttcgcgaa agctatgttg gtcgtctggt cgaactgcgt aagaacaaag
     9001 gcatgaccga aaccgttgcc cgcgaacagc tggaagacaa cgtggtgctc ggtacgctga
     9061 tgctggaaca ggatgaagtt gatggtctgg tttccggtgc tgttcacact accgcaaaca
     9121 ccatccgtcc gccgctgcag ctgatcaaaa ctgcaccggg cagctccctg gtatcttccg
     9181 tgttcttcat gctgctgccg gaacaggttt acgtttacgg tgactgtgcg atcaacccgg
     9241 atccgaccgc tgaacagctg gcagaaatcg cgattcagtc cgctgattcc gctgcggcct
     9301 tcggtatcga accgcgcgtt gctatgctct cctactccac cggtacttct ggtgcaggta
     9361 gcgacgtaga aaaagttcgc gaagcaactc gtctggcgca ggaaaaacgt cctgacctga
     9421 tgatcgacgg tccgctgcag tacgacgctg cggtaatggc tgacgttgcg aaatccaaag
     9481 cgccgaactc tccggttgca ggtcgcgcta ccgtgttcat cttcccggat ctgaacaccg
     9541 gtaacaccac ctacaaagcg gtacagcgtt ctgccgacct gatctccatc gggccgatgc
     9601 tgcagggtat gcgcaagccg gttaacgacc tgtcccgtgg cgcactggtt gacgatatcg
     9661 tctacaccat cgcgctgact gcgattcagt ctgcacagca gcagtaatct cgtcatcatc
     9721 cgcagctttg cgctgcggat atctgaaccg gaaataatca ctatttccgg ttttttattc
     9781 tcttaatctg cattaatcct ttctgattat cttgcttaac tgcgctgcat caatgaattg
     9841 cgccatttca ctttgcatac ttaccacttt gttttgtgca agggaatatt tgcgctatgt
     9901 ccgcaatcac tgaatccaaa ccaacaagaa gatgggcaat gcccgatacg ttggtgatta
     9961 tcttttttgt tgctatttta accagccttg ccacctgggt agttccggtg gggatgtttg
    10021 acagtcagga agtgcagtat caggttgatg gtcaaacaaa aacacgcaaa gtcgtagatc
    10081 cacactcatt tcgcattctg actaacgaag caggcgaacc tgagtatcac cgcgtacagc
    10141 tgttcacgac gggcgatgaa cgcccgggcc tgatgaactt cccgtttgaa ggattaacct
    10201 caggatcgaa atacgggaca gctgttggca tcatcatgtt tatgctggtg attggcggcg
    10261 cgtttggcat tgtgatgcgt acaggaacca ttgataacgg tatcctggcg cttattcgcc
    10321 atacccgcgg gaatgaaatt ctctttattc ctgcgctgtt tattctgttt tcacttggcg
    10381 gcgcggtatt tggtatggga gaagaggccg tcgcctttgc cattatcatc gcaccgctaa
    10441 tggtccggct gggctatgac agtattacca ccgtcctggt gacctatatt gccacgcaaa
    10501 tcggttttgc cagttcgtgg atgaacccgt tttgtgtggt cgttgctcag gggattgccg
    10561 gcgttccggt gctttctggc tccgggttgc gcatcgtggt gtgggttatc gccactatga
    10621 ttggcctgat ctttaccatg gtgtacgcct cacgagtgaa aaagaatcct cttctgtcac
    10681 gcgtgcatga gtccgaccgc ttctttcgtg aaaagcaggc ggatgttgaa caacgtccgt
    10741 ttacctttgg tgactggctg gtattgattg tcctgaccgc cgtaatggtc tgggtgattt
    10801 ggggcgtgat cgttaatgcc tggtttattc cagaaattgc cagccagttc ttcaccatgg
    10861 gtctggtgat tggcatcatc ggcgttgttt tccgccttaa cggcatgacg gttaatacca
    10921 tggcttcatc ctttaccgaa ggggcgcgaa tgatgatcgc ccctgccctg ctggtgggtt
    10981 tcgccaaagg gattttgctg ctggtcggta atggtgaagc gggtgatgcc agcgtgttaa
    11041 ataccatcct caacagcatt gccaatgcca ttagcggtct ggacaacgcg gtcgcggcct
    11101 ggtttatgtt gctcttccag gcggtattta atttcttcgt gacgtccggt tctggtcagg
    11161 cggcgttaac catgccgtta ctggcaccgc ttggcgatct ggtcggtgtt aaccgtcagg
    11221 ttaccgtgct ggcattccag tttggtgatg gcttcagtca catcatttac ccaacctcag
    11281 cttcgttaat ggcaactctc ggtgtatgca gggtggactt ccgtaactgg ctgaaggtgg
    11341 gcgcgaccct acttggactg ctgtttatta tgtccagcgt cgtagtgatc ggcgctcagt
    11401 tgatgggcta ccactaaaaa tgttaagagc cgcaattgcg gctctttttt cattctgccg
    11461 tttcagtctc tacggcttca tttttggcat tgcgcttcat ccacaacgcc agcgctttca
    11521 gcgagtctgg agtgaactca tcgcagcgtg cggtgatttc ttccggcgtc agccagcaaa
    11581 cttcactgac ttcatcttcc tgtagggcga agggaccgtg agagacgcag ctgaacaacg
    11641 cgccccagac acggcaattt ttatcttcga aatagaactg cccgtgctcg gcaaagggga
    11701 caccggcaat gcccaactct tcttccgctt cgcgacgcgc ggattccagc agttgctcat
    11761 cggcctgaac cacaccgcct gcggtcgcat ctaacatgcc gggtaaaaag tcttttgtct
    11821 cggtacgacg ctggaccaga attttgccca tgccatcatg cacgacgatg taagttgcac
    11881 gatgacgcag acactgtgcc cgcatttgtt cccggctggc ttgtgcaatg acttcgttct
    11941 cttcattgac aatatccacc cattcagtac ttgccaaacg acgctgttcc attatcagga
    12001 aaccttcttt ttctggcgct cttacggcgc attttgagtt gtgggtaaat tacggattaa
    12061 tcgcgacctg cgcaatgata ctttgatcat tgagtgcgat tacactaagt acgtcattat
    12121 ccagcatgcc ataactcgcc ggattaccgc ctttcggaat actcaccgag ccggggttga
    12181 agtggaaaat ctccccccgc tgttctgcca ctggcagatg ggtatgaccg tacaccagca
    12241 catcgttctg gtttaaagca ggtagatttt ccgggccaaa aagatgacca tgcgtcaaaa
    12301 acagacgttg tttttccagt aatacctgtt gccacggcgc ggttatcggg aaatgcagca
    12361 gcatttgatc cacttcgctg tcgcagttgc cgcgcacagc gataacctta tgtgccactt
    12421 cattaagccg ttcagcgact ttggctggcg cgtaaccctc cggtaaagca ttacgcgggc
    12481 cgtgattcaa cacgtcgcca aggatcacca gccactgggc accgctttgg gcaaacaact
    12541 ccagaacacg ttccgtcgcc ggtaacgacc catgaatgtc cgatgcaaac atcagtttca
    12601 tcactcactc ctcgtcgaaa aaacgtaagc tatgatactg gattctgtcg ttgctatcag
    12661 ccagattgct tcgccgaaag tgcaataaaa cgctggacag acgctcgctg ccactggaat
    12721 gtcgcataat ccaccaagcg ttctggcacc tcatcgccat gtagtaccag gcggttaatc
    12781 attagcgcca gatcagtatc agcaatgcac cattcaccaa ataaattcgg ctgacccagt
    12841 actaacaaat gttctgccat cgcaaacagt ttctctgcac tggcttttcc ctcggccgtt
    12901 agtggcgctt ttttcgcccc cgcaaagaca acatccgtcg gacgctcttc gcggatgggc
    12961 atcagatcgc tgcgcagcca ggcctgaatc tgtcgtgcac gcgcacgatt ttctaaatca
    13021 agcggataaa tacgttccca ggtcggtggc gcaaatcgat cttccagata ctccgcaatg
    13081 gcagaagatt cactcaactc aaaatcatcg atttgtaata acggcacacg gcgtgtctga
    13141 ccgtaacctt gccacgtcgg ctgcaaatgt tcaccgctgt cgaggtcgat ggtcttgata
    13201 tgaaacgaca ggcctttttc ctgcaacgcc acccaggcgg ataacacata aggggagaaa
    13261 aagtgggcat ctgaccaaag cgtgatagcg ggtttactca ttatgtcctc ggcaataaag
    13321 ggggctggct atcaaactat agagcctttt tgtcactgtc acgtgacgaa tactcacgta
    13381 agtcgccgcg ctttctatac ttgtggtgtt cagatgatta aacaaccgga gctgcaatga
    13441 tcgatctcta tttcgccccg acacccaatg gtcacaaaat tacgctgttt ctcgaagaag
    13501 cagagcttga ttatcgcttg attaaggtag acctggggaa aggcggtcag tttcgtccgg
    13561 aatttttgcg catttcgccc aacaacaaaa ttccggcaat tgttgatcat tctcctgccg
    13621 atggcggcga accgttaagc ctctttgagt ctggtgccat tttgttgtat ctggctgaga
    13681 aaacaggact ctttttgagt catgaaacgc gtgagcgcgc cgccacatta cagtggttat
    13741 tctggcaggt aggcggactg gggccgatgc ttgggcaaaa tcatcatttt aatcacgcag
    13801 ccccccaaac cattccttac gctattgaac gttatcaggt tgaaactcag cgtctttacc
    13861 atgtactgaa caagcggctg gaaaactcgc cctggctggg aggcgagaac tacagcattg
    13921 cggatattgc ctgctggccg tgggttaatg cctggactcg ccagcgaatt gacctcgcaa
    13981 tgtatccggc agtcaagaac tggcatgagc ggatccgttc gcgccctgcc accgggcagg
    14041 cactgctaaa agcacaactc ggtgatgagc gttcggatag ttaacagaaa caggttctcg
    14101 tgtattattt catcctaagt aaaacaacgg agaacctgca atggcacaac ctgccgctat
    14161 tattcgtata aagaaccttc gtttacgtac gtttatcgga attaaggaag aagaaattaa
    14221 caaccgtcag gatattgtta tcaatgtgac gatccactac cccgccgata aagcgcgcac
    14281 tagcgaagat atcaacgatg cgctgaatta tcgcaccgta acgaaaaaca ttattcagca
    14341 tgtagagaat aaccgtttct ctttgctgga aaaattaact caggatgtgc tcgatatcgc
    14401 acgtgaacat cactgggtga cgtatgctga agtggagatc gataaactgc acgcgctgcg
    14461 ctacgccgat tcggtatcca tgaccttaag ctggcagcgt taaccgccat atcgggaggc
    14521 tgcatgaaca tagtgatcac cggagggacg ggattaattg gtcgccattt gattccgcgt
    14581 ttgctggagc tgggccatca aatcacggta gtgacgcgta acccgcagaa agccagttcc
    14641 gttctcggcc ctcgggtgac gctatggcaa gggctagccg atcaaagcaa cctcaacggc
    14701 gttgatgcgg taatcaacct ggccggagaa ccgattgctg ataaacgctg gactcacgag
    14761 caaaaagagc gtctctgcca aagccgctgg aatatcacgc aaaaactggt cgatttgatt
    14821 aatgccagcg acacgccacc gtcggtactc atttccggct cagcaacggg ctattatggc
    14881 gacttaggtg aagtggtggt taccgaagag gaaccgccgc ataacgaatt tacccataaa
    14941 ctctgcgccc gctgggaaga aattgcctgc cgggcgcaaa gtgacaaaac gcgagtgtgc
    15001 ctgctgcgta ccggcgtagt gctggcacca gatggcggta ttctcggtaa aatgctgccg
    15061 ccgtttcgtc ttggcctggg cgggccgatt ggttccggtc ggcagtatct ggcctggatt
    15121 catatcgatg atatggtcaa cggcattctc tggctgctgg ataacgagct gcgcgggcca
    15181 tttaatatgg tttcgcccta cccgatacgc aatgaacaat ttgcccatgc gctcggtcat
    15241 gcgctgcatc gcccggctat tttgcgcgtc cccgcaaccg ccattcggct gttaatgggc
    15301 gaatcttcag tactggtatt aggtggacaa cgcgcgctgc ctaaacggct ggaagaagcg
    15361 ggttttgcgt ttcgttggta cgatttagaa gaggcgctgg cggatgtcgt tcgctgatgt
    15421 ggtttactgc aaacatccgc cagttaactc ccggtgttac agggttagtg gctttgcgcg
    15481 ataagatcgt ctggtgaaag tcgggtcacc atcagaacta actctctgtc taaacctcta
    15541 tccagcatct cctgagcaat acgcagggct tcttcgtgtt tgccctgcat tgcgccttct
    15601 tcacgtaatc tgtcagcaat ggtcatcagt ttctcctttt cttgtggtgc gcgttctgtt
    15661 atctcaccaa taaatgcacg gaaacgctgg gcatcccctg tttgtaatac gtaattaaac
    15721 agggctttta gctgtctgtc attagtgttc cctgtaacta gcagcgaaac aatttggtcg
    15781 actaatccca acagatcccg ctgacgaata tgtttctgaa ttaactccaa cagcgccatt
    15841 ttgcggtgtt gcataatctc gtcatccggc accacggtaa tatccaccaa cggaaacgct
    15901 gatgaatata ttttgcgggc tatagcaggc tcggcaaatt catcaagcca gcagagtgaa
    15961 taaggataag gacttctgca accatgataa aacagcatcg ggatcaccaa tggaagctct
    16021 ttatagcccg catcaagatg gttttgcatt gccgcaatgg aataacgcat catgcgaaaa
    16081 gccattaatt cttccggctt actttggtgc tctatcacta cataaatata acccactccc
    16141 tcctgcgttt tcacagacca caagaggtcg gaataatatt gccgcaggtc ttcatcaata
    16201 aaactgtttg gttccagttt aagcgtcgtt aaatcacaca gtttgcgcag cggcgcggga
    16261 agatgaatat caataaaatc ccgcgcggtg tctggatggc gtaaaaaaga tttaaatacc
    16321 gcatcatgcg gcgtggaagt tgtcgatatt gtcatggcgt tccgtcaccc ctcaaattta
    16381 agatgacgcg acaataacca cgattaacac ccaatgcagc aggcagtttt tatctttgga
    16441 cagcgaattc agaagtattt ttagcggact gcaactttta catttcagcg gaagctgact
    16501 cgcaaattcc ccatgaaaat actccggcag cgttagccac cggagaggaa attatttcag
    16561 cgatccctta aggaactgtt gcagacgagg gctttgcggg ttgccaaata actgctccgg
    16621 cgcgccctct tcttctattt tcccctgatg gaggaaaatg acatgagtag aaacatggcg
    16681 agcaaagccc atttcgtgag tcactaccac catggttttc ccctcttctg ccagttgctg
    16741 cataatacgc aacacttcgc ctaccagttc aggatcgagc gccgaggtag gttcatcaaa
    16801 cagtaaaact tccggttcca tcgccagcgc ccgcgcgata gaaacacgct gttgctgacc
    16861 gccggaaaga tgcaccggat atttcccctg cgcacgttcg tctatcccga cttttgccag
    16921 atacttgacc gcccgctcgc gcgcttcctg cttgctcagg cccaacacct gaatcggcgc
    16981 ttccatgacg ttttccagca ccgtcatatg gctccagaga ttgaagtgct ggaataccat
    17041 cgtcaggcgt gtgcgcagta agcgcagttg atttttatcg gcgactttga gttgaccgtc
    17101 tttgtcgcgc accagattga tcgtctggcc attgaccacg atcgaccctt cactcggttt
    17161 ttcgaggaag ttaatgcagc gcagaaaggt acttttcccc gatcccgacg atccgatgat
    17221 gcttattaca tctccggcat tcgcttgcag tgagacccct ttcagcactt catgttcgcc
    17281 gtagcgtttg tgcaaatcga taacgtttaa tttattctcg gacatcgtat tctcagtgcg
    17341 ttgaagaagg tttcacatgc tgcaaccagc gtttttccgc tctgcgaaag aggctgatca
    17401 ggacataaga gatgattaaa tagagcaccg cggcaatgcc gaaggcggta aaaggttgat
    17461 acgtggcggc gttaatatcg cgggctattt tcagcagatc cggcacagtg gccgtaaacg
    17521 ccagcgcagt agagtgcagc atcaggatca cttcgttgct gtatgccggt aacgcaatac
    17581 gcagcgcaga aggcaaaata atgcaacgat acattttaaa agtcgagaag ccataggctc
    17641 tggcggcttc aatttcccca tgcggaaccg aacggattgc cccagcaaaa atctcggtag
    17701 tgtaagcgca ggtgttaagc gtcagcgcca gcacggtaca gttcaggcca ctgcggaaga
    17761 aagcgttaag gaattcggtt cccttaacaa tctcaagcgt gtacatgccg gaatagaaca
    17821 ccagcaactg aacatacagc ggcgtaccgc gaaaaatata ggtaaataac cagattggaa
    17881 actggatgta tttattactg gagacacgac caatcgccag aaacagcgcc aggactccgc
    17941 ctatcactac cgacaaaata agcagccaca gagtgatcgc cacaccagta aagcgataac
    18001 cgtcggtcca cagcagcggt ttccagtatt catgtaagat ttcgatcaca ggtcagccct
    18061 cttcacaccc acggagtagc ggcgctcaag gaacagcagc acaccattgg aaacagtggt
    18121 gaaaaccagg taaatcacgc cacagacgat ggcgaaatag aacggttccc aggtactttt
    18181 gcctgccagt tgggtggctt tgaccacatc ttccaggccg agtaacgaaa ccagtgcggt
    18241 agatttgagg atcacctgcc agttgttgcc aatgcctggc agggcgtaac gcatcatcgc
    18301 cggaaacatg atccgccgaa acacttgccc acgagtaaaa ccgaacgccg tcgccgcctc
    18361 tatatgccct ttcggcactg ccataaacgc gccacggaac gtttcggtaa aataagcacc
    18421 gtagataaaa ccgagagtaa taataccagc gaccatcgga tcgatatcaa tctgcccgac
    18481 gcccatcgcc tccgtcaccg tgtttagcgc aatctgcaaa ccgtagaaaa tcagcagcat
    18541 caacaccaag tccggcacgc cacgaatcag cgtggtgtac ccttcgaaaa tcagccccga
    18601 aagccgattt tgcgagagct taccgccagc gccaattaaa ccgatgatta cagcgagcac
    18661 tacagaactg atagccagct ccagcgtgac gagcgcaccc tgtaaaataa cacctgaaaa
    18721 cccatacaac atgctgcctg tcctgtcgtg tgtggtgaat taccgcctct tgtctccctc
    18781 cggttacccg aagggagcgg gctttctttc accgatggcg attaaccgcc ataaacatca
    18841 aaatcgaagt actttttcgc taatttctcg taagtaccgt cagcgcgcat ttcggcaaag
    18901 gctttgttca gtgcctcgcg cagttcgtta tcttctttac gcaggcccat gccggtccct
    18961 acgccaaaca gtttttcatc tttaacagac gggccaccga atttgtaatc tttaccgacg
    19021 ggttgtttga ggaaaccttc actggcagcg acctcatcct ggaacgcggc atcaatacgt
    19081 ccggcagtca ggtcagaata aatgttgtcc tgcccctgat acgagacgat ttcaatgcct
    19141 tttggtgccc agtgttcgtt accgaacgtc tcctgggtgg tgccctgcaa tacgcctacc
    19201 cgtttgcctt tcagcgactc gactgtcggc tgaatgtcgg aatttttcgc caccaccaaa
    19261 cgagaatcgg cagcgtacag tttgtcggtg aaggctattt cttgctgacg tttttccgta
    19321 atggaaagcg atgacatgat ggcgtcaatc ttcttcgctt ttaaggacgg gattaacgca
    19381 tccagcggat tttcgacaaa cgtacattgc gtattgatgc gtttgcataa ttcctttgcc
    19441 agatcgatat cgaagccaac cagttcgcct tgtgaatttt ttgattcaaa tggcgcatag
    19501 gtcgggtcgg taccgatgcg gatgttttgc ggaatcgcag caaatgccgc agttgcgctg
    19561 gagaaggcca gaaccagaga gagcgatagc accagttttt tcataatgtc ctcaactgac
    19621 agtcttttta tagggaatat ttacaggttt gttacactta tcgtgccata aaattgacca
    19681 tcaaggcaaa atatcccgcc ccgttagacg tattattttt cattaaaaat gcttaagtat
    19741 gcatttcaat gcaccacgat agtgcaacaa acctggcgtg caccatgatg atcattccat
    19801 caggtacagc ttcccagcga cgtatcagtc accgtagaca ttaaagtcga aatacttttt
    19861 cgccatcttg tcgtaggtgc cgtcctgacg cagctcgcca agcgccttat tgaaggcagc
    19921 cgtcagttca gcatcatctt tacgtagccc tacaccggtg ccatcaccga agtatttttt
    19981 gtcttttact gatgagccag caaaggcgaa atctttacca gcaggttgct tgaggaatcc
    20041 ttcgctggca gcaacttcat cttgtaacgc agcatccaga cgtcctgcag ccagatcgga
    20101 atagaccaaa tcctggttgg cataggccac cacatccacg cctttgctac gccaggtctc
    20161 gttagcgtaa gcttcctggg ttgatccctg cagcacacca acatgtttac ctttcagtga
    20221 atccagcgtt ggctgaatcg gtgaaccttt ggcagcaatc aaacgagaat ctgcggcgta
    20281 cagcttgtcg gagaaggcaa tctcctgctg acgtttatcg gtaatggaaa gcgacgaaat
    20341 aatggcgtcg atttttttcg ctttcagtga ggggatcagc gcgtcaaagt cactggcaac
    20401 ccaggtacat ttcacctgca tccgtttgca catctcgtta ccgagatcga tatcaaagcc
    20461 aacaaaatca cctttagcat ctttcgacga gaacggcgca taggtggtat cggttccgat
    20521 acgtaccgtc tccggtagcg ccgcatagct ggaagccgct gcggagagac cgactaacaa
    20581 agacagagcg agaatcgact tcttcataca taaccctcaa gtgaaaatgg cttttttatg
    20641 ttgtgtattg tgttgtgtgt ttgcaggctc tttcatgcag gtcttatgcc atcttgacga
    20701 caacagtaac attcaacgtt aaatatgtta ataagacgtt gcattattgt cctgaagttg
    20761 aagatagcag gtatagcggt tgaatcgcag cgtttcgatt gaatggcaga aacaaaatgt
    20821 cgaggatttg atcgcggttg caaaattgcc ctgaaacagg gcaacagcgg agttatgcgc
    20881 cctgccagcg ggcaaagaga tcttcaggaa gggttatcgc aaactggtca agaacacgat
    20941 taaccgtctg atttatcaca tcatcaaggg attgcgggcg atgataaaac gccggaacgg
    21001 gaggcataat caccgcaccg atttctgccg cctgagtcat taaacgcaga tggcctaagt
    21061 gcaatggtgt ttcacgcacg cagagcacca acgggcgacg ctctttcagc accacatctg
    21121 ccgcacgggt cagtaaacca tcggtgtagc tatggacaat gccggaaagg gttttgattg
    21181 aacagggtaa aataaccatc cccagcgtct ggaaagaacc ggaagagatg ctggcggcaa
    21241 tatcgcgcgc atcgtgcgtg acatcggcta atgcctgcac ttcgcgcaga gaaaaatccg
    21301 tttcgaggga taaggtctgg cgcgctgcct ggctcatcac cagatgcgtt tcgatatctg
    21361 tgacatcgcg cagaacctgt aataagcgca cgccataaat cgcgccgctg gcaccgctga
    21421 tgcctacaat gagtcgtttc ataaatagtt gccctgtcag acttgcaggc agactttgca
    21481 ggatttcggc gggagttgca agtcagggtg ccagaccggc accctcagcg aaggcatcat
    21541 ccttcgttat gcatttcgag attttccact tcgttctgac gttgcactgc tttggcgtca
    21601 tcattacgta acgtatcgag gaaatcgagg tagccctgat caacatcttt ggtgacgtag
    21661 acgccgttga acaccgagca ttcaaactgc tggatatccg gattttcagc gcgaacggcg
    21721 tcgatcagat cgttcagatc ctggaaaatc aacccgtcag caccgatgat ctggcgaatt
    21781 tcatcaactt cgcgaccgtg agcgataagc tccgtggcgc tcggcatatc aataccataa
    21841 acgttcggga agcgaatttc cggtgccgca gaagcgaggt acactttctt cgctccggct
    21901 tcgcgtgcca tctcgataat ctgctcagaa gtggtgccac ggacgatgga gtcgtcgacc
    21961 agcaggacgt ttttatcgcg gaactcggcg cggttggcat tcagtttacg gcgcacggac
    22021 ttacgacgca gctgctggcc cggcatgata aaggtgcggc caacatagcg gtttttaacg
    22081 aagccctggc ggtacggttt gcccagaata cgagcaattt ccagcgcgat atcacacgag
    22141 gtttctggga tcgggatcac cacgtcgata tccagatctt cccattcgcg ggcaattttc
    22201 tcgcccagtt tcgtgcccat attcacacgc gcgctgtaaa cggaaatttt gtcgatgaac
    22261 gagtccgggc gagcaaagta tacatactca aacaggcacg gattgctgac cggattgtca
    22321 gcacattgac gggtaaacaa ctgcccttct tcagtgatgt aaatcgcttc gcccggcgcg
    22381 acgtcacgca ggaaatcaaa gcccagcgta tcgagcgcta cgctttcgga agcgaccata
    22441 tattctgtac ggttctcgtc aatatcacgt tttcccagta ccagcggacg aatcccgttt
    22501 ggatcgcgga aagcaaccat accgtggccg ataatcatcg ccacacaggc atacgcgccg
    22561 cggattaagc ggtttgtggc agcaatggca gcgaaaatat tgtcggcttc cagcgggtag
    22621 tggcggaaat tgtccagctc gctggcgaag atattaagca gaatttccga gtcggaagtg
    22681 gtgttgatgt ggcggcgttt ttcttcaaac agttttttac gcaactcgtg agcgttggtc
    22741 agattgccgt tgtgggcaag cgtaatgcca tacggggagt taacgtaaaa cggctgcgct
    22801 tcagaggcgc tggagctgcc agccgtaggg taacgcacat gaccaatgcc catattgccc
    22861 tgcaaacgct gcatatggcg agcttcaaat acatcgctca ccagcccgtt cgctttacgc
    22921 aaacggaagc agttattggc atctatggtg atgatgccgg cggcatcctg accgcgatgc
    22981 tgaagcaccg ttaaggcatc ataaatcgac tggttaaccg gcataacacc ggcgataccg
    23041 acaataccgc acatacgtct ttttcctcgt taagccacat ctcagagcac ttacgctctg
    23101 ggcaagaaac ttgacgagct ttgcagataa tcaaaaaacc atctgatgat aaaactgaat
    23161 tgcgggatca gctgtgattt gctccagtct tcgcttttcg acaccccggt aaaggagtcg
    23221 agaaagaaga gaatggcggc aacaatcaac acaccgcgca acgcaccgaa acagacgccc
    23281 agcacccgat cggtgcctga caaccccgtt ttctccacca actggcctat cacgaagttc
    23341 acgatagcac caacgatcag ggtagcgata aacagtaccg cgatggcaat cccatttcga
    23401 accagttcgt cttcaaagcc cgtaaaccag actgacaggt aagtgtagta atgactggca
    23461 acaaagaaag cacaacccca tgtcaccagc gataacgctt cacgaacaaa gccgcggatc
    23521 aggctaacca gagaggaaaa agcaatcacc gcgattatgg cgtaatcaat ccagaccata
    23581 tgtgtcccac gattttacgc cctgtcatcc tgttcggggc gcattctaac agaaaaagaa
    23641 aacgtttgcg tagggatttc cttcccacgc atcaataaaa atggcgctga aaaaatattc
    23701 aacgccatcg actttttatg cctttgcggc atcgtgcaat gcgtgtcgga tgcggcgtaa
    23761 acgccttatc cgacctacgg ttctaacact gcccaggcct gataagacgc gccagcgtcg
    23821 catcaggcaa gactgtatta attcggcgta tagcccatta ccacgccact taagccagaa
    23881 agttgcttca actcacccag cgaacctttc agcttatctt tcgaggcatc cggcccaacc
    23941 agaatacggg taattttacc ctgcactggc gtggatggcg acgtataaac ccgataaccg
    24001 gcaccgcgca gcttaccgac aatctcattc actttatcgg catttttcag cgcacccagt
    24061 tgcacaacat aggctttacc cgtcggtgca gctttttctt ccacgaccgg cttcggttct
    24121 ggcttcggcg caggtggtgc ttcaaccttt ggtttaggcg gctccaccgg tttcggcttc
    24181 ggtggggcga ccggtgcagg ttccggttca aactcggtgt tattggctgc aatagtggcc
    24241 ggatcgagcg acggtgctgc ggcatcacct gcccgcacct cttccgctgc gccttccggc
    24301 ggctgcgtcg gcaacgcctg ggtggcggct ggcatcatat caggctcatc gcgatcgccc
    24361 gcttttggaa ccagcgggat agccgcaaac tcatcctgat aatgtttttt ctgcccgtcc
    24421 agcagccctg gaagtacaat cacccccagc gccaccagca cgatcgtgcc cactaaccga
    24481 ttctgaaact tacttgccac cgcttctcct cgcgtcaatc acttccatga catgtgcgac
    24541 cgtgtggaaa gaaccacaca ccagcacggt gtcttccgct ttagcgtccg ccattgcggc
    24601 atcccatgcc tgcgcaacgc tatcaaatga tttgccgtta cccaaatgct caagcagttg
    24661 ttctgccgtg gcaccgcgcg gcccttccag tggcgcacaa taccagtcat caaccacgct
    24721 tttcaaccag gccagagttc cggcaatatc tttatcatgt agcataccga taaccgccag
    24781 cacgcgcccg tttttcggta aggctttcat acgcccggtg agatattccg ccgcatgtgg
    24841 attatgcgcg acatcaaaaa taacgcgtgg cgactcgctc acaatctgga aacgtcccgg
    24901 taaaattgcg ctggcaatcc cgtcgcgaat ggcattttca ctgacctcca gcccgctggc
    24961 acgcagtgcc gccagcgccg ttgcggcatt cggttgcggg acaagcggca acggcaaatt
    25021 ttccagcgtg ccgtgagcat cgctaaacgt ccagtcatga tcggtgacgg aatagttcca
    25081 ctcaacgccc cgacgttgta acagtgcacc tttttcctgc gccacatcag caatggtaga
    25141 aggcatttcc ggctcaccga caattgccgg tttttcgctg cgaaagatgc ctgctttctc
    25201 gcgaccaata ctttcgcgat ctggccccag ccagtcggta tgatccagcg caatgctggt
    25261 tactaccgcg acatcggcgt cgacaatatt ggttgcgtcc agacgaccgc ccagccctac
    25321 ttccagaatc accacgtcaa gttgtgcctg tttgaacagc cacaacgccg acagcgtacc
    25381 gtactcgaaa taggtcaggg aaatatcacc gcgtgccgat tcaatctccg caaaagaggc
    25441 ggtgtgggcc gattccggca attcctgacc ctgcacacgt acgcgctcgg tataacgcac
    25501 cagatgaggc gaactgtaga cgcccacttt gtaccctgcc gccatcagaa tcgactccag
    25561 cgtacggcag gtggtgcctt tgccattcgt acccgcaacg gtaaacacaa atggcgctgg
    25621 tttcagaacg ccaagacgcg ccgcgacctg gctcacgcgc tcaaggccga gatcgatagt
    25681 tttactgtgc aggttttcca gataagaaag ccacgaagcc agaggcgacg cggcttgagg
    25741 agtgcgtttg ataatcatgg tatccgctga ttcgttacgg tgagaatagc aaaagggcag
    25801 agccagtggc cctgccctta tcagttatca ggcctcaggt tcctgatccg gtaccggggg
    25861 taccactacg ccttcacgcg gcgcttcagg attcggcgct ggcagattca tcaacttcgc
    25921 cagaatgctc gccagtttca ggcgcatttc cggacgacgg acgatcatgt cgatcgcgcc
    25981 tttctcgatc aggaattcac tgcgctggaa tcctggcggc agtttttcgc gaacggtctg
    26041 ttcgataaca cgcggaccgg caaagccgat taacgctttc ggttcagcga tgttgagatc
    26101 gcccagcatg gcgaaacttg cagaaacacc gcccatcgtc gggtcggtca gcacggagat
    26161 gtacggcaag ccgcgctcct gcatttttgc cagtgccgca gaggttttcg ccatctgcat
    26221 cagcgacatc agtgcttcct gcatacgtgc gccaccagag gcggagaagc agatcagcgg
    26281 gcagttatct tccagcgcct gctcaacggc acgcacgaaa cgtgcaccca caacagaccc
    26341 cattgaaccg cccataaagg cgaactcgaa tgccgcagcg acaaccggca ttccatacag
    26401 agtgcctttc atcaccacca gcgcatcttt ttcgccagtt tctttctgcg cagatgccag
    26461 acggtcttta tacttcttgg agtcacgaaa cttcagcaca tctttcggct caagctcgct
    26521 acccagctcc acaaggcttc cttcatctaa caggctatgc aggcgattac gcgctgtcat
    26581 acgcatgtga tggtcacact tcggacagac ctcaagatta cgttccagct cagcgcggta
    26641 taaaacctga ccgcagctat cacacttagt ccacacccct tcaggaatgc tcgccttgcg
    26701 ggtgggagta atgttgcttt taattcgttc aatccagctc attagggacc tttctgtctg
    26761 aacctggttc gatgccagtt ttatctttgg ggacgcataa tgccattttt gcccccaaca
    26821 gaccatgaat gttgcacatt aaaacataac agcccgaaac tttggataaa aaagtggtcg
    26881 aaccgcggag ttacttttta ttttgcggcg cgtgccgcag cgcgtttgtg acgaataaat
    26941 tcgaaaaccc caggcaatat tgaaacaaaa ataatgccaa caatcatcag cttaaggttg
    27001 ctctgaatga aggggagcgt gccgaagaaa taacccgcgt aggtaaaaat cagtacccac
    27061 aacagtgcgc cgatcacgtt ataagcggcg aaatgacggt acgacatgtg gcccattccc
    27121 gcaacaaacg gcgcgaacgt tctgacgatc ggtacaaaac gggcgagaat aatcgttttg
    27181 ccgccatgtc tctcgtaaaa ctgatgagtc ttatcaagat aactgcgacg aaagattttc
    27241 gaatccgggt tactgaacag tttgtcacca aataaccgtc caatggtata gttcactgca
    27301 tcaccgacaa tagcggcaat caacataagc accaccatca tatggacatt gagatcgtta
    27361 gtttccagcg acgccaatgc accagcaaca aacagcagcg aatcaccagg caggaatggc
    27421 gttaccacca gccccgtttc gcagaacaga atcaaaaaca gaatggcata aacccagacg
    27481 ccgtactccg cgaccagctc tgccagatgc acgtcaatgt gcaggataaa atcgatgagg
    27541 aaataaatca ggtccatatt tgcctatgcc ttgtactcgt catctttcag gctgtaacta
    27601 cgttggctac agcctgaaat gttccgagca ttattctcgt tagtccgcca gaaatagcgg
    27661 gcccattggc ggttttggaa gatcataccg gtcagggtaa tccaccgcga ccagatacag
    27721 cccttccgct tttgccgttg ctgccgccag cgttctgtcc tttgccgcca gcaactctgc
    27781 tatccagctc tccggctggt tgtgggcacc gacttccatc aggctgccga caatattcct
    27841 gaccatatga tgtacaaagg cattcgcttt gatatctacc accacataag gaccgtgacg
    27901 cgtgacatta atatgcataa cgttacgcca cggggttcgg gactggcact gcaccgcacg
    27961 gaacgaggtg aaatcattct cgcccagcaa gcattgcgca gcccgatgca tccgttcagc
    28021 gtccagcggt tcgtaaaaat gggttacccc tttactcagt accgccgggc gcagccgatg
    28081 attgtagatg atgtagcgat aacggcgagc cgtggcgcta aatcgggcat gaaaatcatc
    28141 aggtacagct ttaacccaac gcacggcgat gtcaccaggt aaattcgcat ttacgcccaa
    28201 ggtccacgcc gcgtctttgc gctgcgcggt tgtttcgaaa tgcaccactt gcccggtacc
    28261 gtgtacccct gcgtcagtac gcccggcgca gaagacggtg atgggttcgt tcgccacctg
    28321 ggagagcgcc ttttccagct tctcctgcac actgcggact tcattctgcc gttgccagcc
    28381 gtaatactta ctgccgtcgt actcaatgcc cagcgcaatt ttataaactg gcagttgttg
    28441 ctggtcggac attagtacag atactcctgc accagtttct cggcgatttt tactgccatc
    28501 agcgcgccgc caaagcgaac gttatcggcc accgaccaga actggacttg ctccggcata
    28561 ccgtagtcat tacgcacgca gccaacagaa agatgcggcg tacccgaagc atcccctacc
    28621 tgagtcggga attcgttctc ttcagagagc acaatatctt cgccctgagc aaacgcatca
    28681 cgcgcttctt ctgccgccag cggacgcagt gcttcaaagt tgaccatctg ggcatgaccg
    28741 tagaataccg gtgcctggac gacgcttgcc gaaatcatca gcccttcgtc ctgcaggatt
    28801 ttgcgtactt cgtcaacgat acgacgttct tcacgcacgc taccttcgct atccggcaac
    28861 aacggcagca tattgaacgc cagttgacga ccgaagaaat cttcttcgtc aatcggaatg
    28921 ccgttgagca atttcgcact ctgccccgct aacgcatcga ccgctttttt gccctgggcg
    28981 gaggctgaaa tcaggctggt aacactgata cgcgacaaac cgccctgatc gattaacggt
    29041 ttcagtgccg ccagcagctg gctggtcaga ctgtctggta cggcgatgac attccggttg
    29101 cggtaatcgg tcagtacaaa cgggtttact tccggcacca ccagcggtac gtcgggttcg
    29161 agagcaaaca gtccgctgct gtcgatcacc aggcaacctg agttggtcgc ttcttcaacc
    29221 caggcagcgg tagcttcttt gcctgcgaca aaaaatgcca gctgcgcctg cgtccagtcg
    29281 aattcagcgg catcctgcac ggtgattgtt ttaccaccaa agcgcagttg ttcgcctgcg
    29341 ctttcgttac gtgccagtgc ataaatttcc ccaaccggga actgacgttc agccagcgtt
    29401 tcaagcaggg cttcgcccac agcgccagtt gcgcccagga cggcaatgtt ccagccttca
    29461 gacatggtgg tttactccag aaatagcaaa gctccctgcc aatgttacag cagagagcat
    29521 gaagaagaga ttaacgtgcc ggatgatgaa cggcgttaaa acccagttta cacagcaatg
    29581 atgccgcact ggcgtcatca caaattacat acagagatga ccattcacgg cgctcaagat
    29641 agtttttgcg cagtttatcg aactcacccg gtatcccggc gactttacgc agcggtgcgt
    29701 catcgcggcg cacatcatac accaaatgca ccagcctttt cagcgtcggt tgatcgagcg
    29761 ggccatgcag cgtaatgcga ccaaactctg gcgcgggcag taatgtatcc agcgcaacgt
    29821 gctgttcatg cccaataaac ttgctatagg cttcaaacac ttgcgtagta ccgcgtgctt
    29881 taccttccag ggtataacct gcgatatgcg gcgtgccgat atccactttt gtcagcaact
    29941 ccacgttaag ttccggttcg ccttcccaga catccagcac tacgcttaac ttctggcctt
    30001 cattcaggca ggtcagcaac gcagtattat cgacgactgc gccacggcag gcgttaatca
    30061 gaatcgctcc cggcttcagg ctacggatca gtttttcatc cgccagatgc agcgttttgt
    30121 acggcccatc tttgaagagt ggcgtatgga aagtcagaat atccgcatgc tgaactaatt
    30181 catccagcga gcggaaatca ccctcatccc cacggtcagc gcgaggcgga tcgcaaagca
    30241 atgttttaat ccctaacgct tccagtcgcg cctgcaatcg acgtccaacg ttaccaacgc
    30301 ccacgatccc caccgtacgg tcgtgcagtg aaaatccatc gcgttcggca agcatcagta
    30361 gtgaggagaa aacatattcc accaccgcaa tcgcattaca gccaggtgca gcggaaaaac
    30421 caattcccgc ctgcttcaac catgcttcat cgacatggtc ggtccccgca gtggcagtgc
    30481 caacaaattt aatgggtttt cctgccagca aagattcatt cactttcgtg accgaacgca
    30541 ccatcagcgc atccgcgtct gccagttgag cgacggggat tggacgcccg ggaaccgcgg
    30601 tcacctcacc caaacggcta aataagtcgc gggcataagg catattttca tcaacaagga
    30661 ttttcacgtt tgtgttacct gtatgagacg agagttaacc ggacaagtgt gccataatct
    30721 cgcggccagg catacttgcg aagatttcag gtataaggat acgtaatgat acaacctatt
    30781 tccggccctc ctcctgggca accaccaggt cagggagata acctgccgtc tggcgcgggc
    30841 aatcagcctt tatccagtca gcaacgtact tcgctggaaa gcttaatgac gaaagtgacc
    30901 tcactgacgc aacagcaaag agcagaactg tgggcgggta tcaggcacga tattggtctg
    30961 tcgggagatt caccgctgct ttcgcgtcac ttccctgccg ctgagcataa tctggcgcaa
    31021 cgtctgctgg ccgcgcaaaa aagccattct gcccgccagc ttttagcgca attaggggag
    31081 tatttacgtc tggggaataa tcgtcaggcg gtcacggatt atatccgtca taactttggt
    31141 cagacgccgc tgaatcagct ctcaccggag caattaaaaa ccattctcac cctgttgcag
    31201 gaagggaaga tggttattcc gcaaccacag cagcgcgagg cgaccgaccg tcctttatta
    31261 ccggcggagc acaatgcgct aaaacagctg gtgaccaaac ttgcggcggc aacgggggaa
    31321 cccagcaaac agatctggca atcgatgctg gaactttccg gggtgaaaga tggcgagtta
    31381 attccagcga aactgtttaa ccatctggtg acctggctac aggcgcgtca gacgctaagc
    31441 cagcaaaata cgccgacgct ggaatcacta cagatgacgc taaaacaacc tttagatgcc
    31501 agtgaactgg cggcgttatc ggcatatatc cagcaaaaat atggtctttc tgcgcaatca
    31561 tcgctttctt ctgcccaggc cgaggatatt cttaatcagc tttatcaacg gcgggttaaa
    31621 gggattgatc cgcgtgttat gcaaccgctg cttaatcctt ttccaccgat gatggacacg
    31681 ttgcaaaata tggcaacgcg tcccgcgctg tggatactgt tagtcgcgat tatcctgatg
    31741 ctggtctggc tggttcgtta accccgacga aatgacagta tcgtgacaat aatacccagc
    31801 accgcagaga tcgccccggc aagaaatacc gaagagtaac caaacgtggt cgccagcatt
    31861 cccgccagcg gcccggagac gccgagggcg atatcctgaa acgcggcgta accgcccagt
    31921 gcggtgccgc gaacttgtga ggggacgcgt ttaaccacct ccacgcccag cgcaggaaag
    31981 ataagcgaac atccggctcc ggttaacgcc gcgcccgcta atgcgaccca tgcacctggg
    32041 gcttgccaga gcagcaacaa gcccaccgtt tctacaagca gagagacaat cgccactttc
    32101 acgccgccaa aacggtccgg catccagcca aacatgacgc gcatcacgac aaatgcgccg
    32161 ccaaacgcgg taagagtaaa gcccgccatc gcccatcctt tgctggcaaa gtagagcgaa
    32221 acgaaagtcc cgataacagc aaaaccaacg ccttgtagtg ccagacctaa ccctggtttc
    32281 cagataagcc cgacaacgct ccacagcgat ggacgttctc ccgccagggc cggtactttg
    32341 cgcactgtgc cgttacaggc ccacgccagt acgggtaata ccattgtggt gatcgccagt
    32401 gcggcaaaac cgtaatggct atgaatcaac aggccaagcg gagcaccaac agcgagggca
    32461 ccgtaaatcg ccattccgtt ccatgacatc actttgccag agtgttttgg ccctacgatg
    32521 cctagtcccc aggtcagagc gcctgtcagt aactggcttt caccaaagcc aagaatcaaa
    32581 cgcccgacga ccaacagagc aaatttgaac ggtgcggaga caggcaaaat cgccgccagc
    32641 agcaacgcgc cgccagccag accacaagct aacattccct gaagcgccga acgttttgca
    32701 ccatattgat cggccagtcg cccggcgtaa ccacgcgtca gcaccgtagc cagaaactga
    32761 atcccgacgg caatgccgac catggtattg ccatagccca gttcatgatg aacaaacagc
    32821 gggataaccg gcaacggcaa ccctacggtc atgtaggtga gaaaaaccgc aaaagcgatg
    32881 cggaagagcg aaaaattggc agaagatcgt gtttcggttt ggcttacagc agtcatgcat
    32941 tactccagaa tgcagcgcaa ggcgaggagt atccccgtct catctctctg gtttcagggt
    33001 tacggtgcgt tggcaggatt taacgcgtac gtcttttcag aaggaaatcg acaaagcggg
    33061 aagtttgcct ggaactggcg gcgattgtca atgatgtgaa aaagggaacc atcaggttcc
    33121 cttttgcgtt agtgccggag gccgcattgg cgcgtaacgt cggatgcgac gctggcgcgt
    33181 ctactccgac ctactgcgaa ttaatctttc agcttgcgca ttaccagcgt ggcgttggtg
    33241 ccgccgaagc cgaagctgtt agacataacg gtggtcagtt cgcgatcggt cgtttcggtc
    33301 acgatgttca gacccgcagc ctgctcgtcc agctcttcaa tgttgatgct cggggcgata
    33361 aagccgtgtt ccagcatcag cagagagtag atagcttcct gtacgccagc agcgcccaga
    33421 gagtgaccgg tcatggcttt ggttgcagaa atcgccgggc tcttatcgcc gaacacttca
    33481 cggatagctg ccagctcttt cacgtcgcca accggagtcg aagtaccgtg ggagttcagg
    33541 taatcgattg gggtatcaac gccatgcatc gccatcttca tgcagcgtac tgcgccttcg
    33601 ccagacggag caaccatgtc tgcaccatca gaggttgcgc cgtagccaac gatttcagca
    33661 tagatgtgag caccacgcgc cagcgcgtgt tccagctctt caaccactac cataccgccg
    33721 ccgccagcga taacgaaacc gtcacggtga gcgtcgtaag tacgggaggc tttttccggg
    33781 gtgtcgttgt atttagtaga cagcgcaccc attgcgtcga attcgcaagc catttcccag
    33841 cacagctctt cgccgccgcc agcaaacacg atgtcctgtt tgcccagttg gatctgctct
    33901 actgcgttac cgatacagtg tgcggaagtc gcacacgcgg agctgatgga gtagttaacg
    33961 ccatgaattt taaacggggt ggcgaggcag gcagaaacgc cggatgccat cgctttggtg
    34021 accacatacg ggccaaccgc tttcaggccg cgcgggccgc gcattgcgtc agcgccgaac
    34081 acctggaaac gcggggagcc gccgccggaa cctgcaatca ggccaacgcg cgggttattc
    34141 tggtaagctt ccggagagag gcccgcatca gcgattgcct gctccataga aaggaatgca
    34201 taaatggatg cgtcgctcat aaagcgcaca actttgcggt caatgaggcc agtggtatcc
    34261 agttttacgt tgccccagac gtggctacgc atgccggaat ccttcagctc ctgagagaaa
    34321 gtgatccctg aacgtccttc acgcagagat gccaggactt cctgctggtt attaccgatg
    34381 ctggaaacaa tgcccaggcc agtaatcact gcacgtttca ttcaatacct ctgtaagtcg
    34441 cacatagagt aagtttcgaa tgcacaatag cgtacacttg tacgccgaac aagtccgatc
    34501 agccatttaa tagagaaatt tgcgcagcct tacacacatc gctaagatcg agccaccgcc
    34561 tgtaagacga gtaacttacg tgaaacacta ctccatacaa cctgccaacc tcgaatttaa
    34621 tgctgagggt acacctgttt cccgagattt tgacgatgtc tatttttcca acgataacgg
    34681 gctggaagag acgcgttatg tttttctggg aggcaaccaa ttagaggtac gctttcctga
    34741 gcatccacat cctctgtttg tggtagcaga gagcggcttc ggcaccggat taaacttcct
    34801 gacgctatgg caggcatttg atcagtttcg cgaagcgcat ccgcaagcgc aattacaacg
    34861 cttacatttc attagttttg agaaatttcc cctcacccgt gcggatttag ccttagcgca
    34921 tcaacactgg ccggaactgg ctccgtgggc agaacaactt caggcgcagt ggccaatgcc
    34981 cttgcccggt tgccatcgtt tattgctcga tgcaggccgc gtgacgctgg atttatggtt
    35041 tggcgatatt aacgaactga ccagccaact ggacgattcg ctaaatcaaa aagtagatgc
    35101 ctggtttctg gacggctttg cgccagcgaa aaacccggat atgtggacgc aaaatctgtt
    35161 taacgccatg gcaaggttgg cgcgtccggg cggcacgctg gcgacattta cgtctgccgg
    35221 ttttgtccgc cgcggtttgc aggacgccgg attcacgatg caaaaacgta agggctttgg
    35281 gcgcaaacgg gaaatgcttt gcggggtgat ggaacagaca ttaccgctcc cctgctccgc
    35341 gccgtggttt aaccgcacgg gcagcagcaa acgggaagcg gcgattatcg gcggtggtat
    35401 tgccagcgcg ttgttgtcgc tggcgctatt acggcgcggc tggcaggtaa cgctttattg
    35461 cgcggatgag gcccccgcac tgggtgcttc cggcaatcgc cagggggcgc tgtatccgtt
    35521 attaagcaaa cacgatgagg cgctaaaccg ctttttctct aatgcgttta cttttgctcg
    35581 tcggttttac gaccaattac ccgttaaatt tgatcatgac tggtgcggcg tcacgcagtt
    35641 aggctgggat gagaaaagcc agcataaaat cgcacagatg ttgtcaatgg atttacccgc
    35701 agaactggct gtagccgttg aggcaaatgc ggttgaacaa attacgggcg ttgcgacaaa
    35761 ttgcagcggc attacttatc ctcaaggtgg ttggctgtgc ccagcagaac tgacccgtaa
    35821 tgtgctggaa ctggcgcaac agcagggttt gcagatttat tatcaatatc agttacagaa
    35881 tttatcccgt aaggatgact gttggttgtt gaattttgca ggagatcagc aagcaacaca
    35941 cagcgtagtg gtactggcga acgggcatca aatcagccgt ttcagccaaa cgtcgactct
    36001 cccggtgtat tcggttgccg ggcaggtcag ccatattccg acaacgccgg aactggcaga
    36061 gctgaagcag gtgctgtgct atgacggtta tctcacgcca caaaatccgg cgaatcaaca
    36121 tcattgtatt ggtgccagtt atcatcgcgg cagcgaagat acggcgtaca gtgaggacga
    36181 tcagcagcag aatcgccagc ggttgattga ttgtttcccg caggcacagt gggcaaaaga
    36241 ggttgatgtc agtgataaag aggcgcgctg cggtgtgcgt tgtgccaccc gcgatcatct
    36301 gccaatggta ggcaatgttc ccgattatga ggcaacactc gtggaatatg cgtcgttggc
    36361 ggagcagaaa gatgaggcgg taagcgcgcc ggtttttgac gatctcttta tgtttgcggc
    36421 tttaggttct cgcggtttgt gttctgcccc gctgtgtgcc gagattctgg cggcgcagat
    36481 gagcgacgaa ccgattccga tggatgccag tacgctggcg gcgttaaacc cgaatcggtt
    36541 atgggtgcgg aaattgttga agggtaaagc ggttaaggcg gggtaatctg ctctcgcagt
    36601 gtttgccgga tgcggcgtga acgccttatc cgacctacgt gtgacgtgta ggcctgataa
    36661 gacgcgataa gcgtcgcatc aggcatggtg ctccagacgc cgcaacttac tgctgtgacg
    36721 cctgctgaaa caggttttcc cacatatcgg tcaccagcgc ctggtcacgc ggcgacaatt
    36781 caccggcacc aatggctttt tccagactct ggctaacggt cgtatgcacc gcctgagcgg
    36841 agtggtcatc accactttcc agttctgcga tggctaatgt caggtggcca cgcaaatacc
    36901 cactggcaaa caactcatca tcactggcat ggtcaaccat atcgtcgatt aatgccagaa
    36961 tgcgtgattc aaactccgcg atcatcttct ttcctcattg taaaacgtgg cgctggcttt
    37021 agttgagcgc ttccggccac gggaactgtt ccgccgtaag ttccggcgtg tgataataat
    37081 tttgtagtgc tttaatgaag cgtgccgggc gttcaggaat gccgtttgct aggtaatcca
    37141 tcacctgcgc atgtacccgc cgttgaaaca ccacacgatc cggttcgaag tcgccttcca
    37201 gattgtcgca actgacgtta aacggatatc ccgccgccac acagaacaac cagtcgaacg
    37261 cctgcggctt cacttcaaca tcttcaaact ggctttgcgt ttgggcatcg cgtccgtccg
    37321 ggcaatacca gtagccgaaa tcaaccagct cacggcgcgc tttcccggca atacaccagt
    37381 gcgaaatctc atgaattgcg ctggcataaa agccatgagc aaagacgatt cggttatacg
    37441 gtacttccgc atcagcagga agatagatcg gttcgtcgtc gcctttaatc agacgggtat
    37501 taaattcatc ggcaaagcag ctattaaaaa tttcaatcaa ctgctcatag tggtgtgtac
    37561 tgttcattag ttcatcccca accagtggag gatctcctgt ccgtggctat caaaaagtaa
    37621 tttggcactc atcaccgctg agacaataac aatcatcggg cggatcagct tttgtccttt
    37681 gctcaacacc agtcgtgaac ccatgcgcgc gccgaggaac tgccccacca gcatgacaaa
    37741 gcccgtcgcc caaatcactt tgccaccgag aataaacagc agtaaaccgc cgatgtttga
    37801 cgttgcgttg agtaatttgg cgtgcgccgt ggctttggcg aggttgaatc cgcacagcgt
    37861 aacaaaggcc agtgcgtaaa acgatccggc tgccggacca aagaatccgt cataaaaacc
    37921 gacgcagcca ccggcaatta acgcgaaggg taaaccgtac atccgacgct ggcgatcttc
    37981 ttcacccagc tttggcatca gcaagaaata gaggccgata caaattacca gaatgggcaa
    38041 aatctgccgt aagacatcag cctgaacgta ttgcaccagc agtgcgccgc tcattgagcc
    38101 gacaaaggtc atggcgatat tgagtttctg atcgcttaaa ctaaccactt tgcggcgaat
    38161 aaagtagata gtggcggaaa tagagccgcc gcaggcttgc agtttattgg ttgccagcgc
    38221 gttagcggga gacattcccg cagccatcaa tgccggaatg gtgagtaacc caccgccacc
    38281 ggcaatcgag tcgataaatc ccgccagcat ggcgacaaaa aagagaactc ccagcaacag
    38341 cggggaaacc ataaacaggc tattaaacgt ttccattaga tcacgtgctc atccagtagc
    38401 gcctggcagg aaggcggcaa cggaggcggt gtcttcttct caggctttgt tgttcccggt
    38461 tttggaggtg caaaccagct ttgcagttct gccccgcaac catcgcctgg tggcggtaaa
    38521 ggttgatctt cacactccag actatcggca ggacaacgta atcgtacatg catatgcgcg
    38581 cgatgctgga accagggtcg cactttgcgc aaccagtcgc gatcggtgcc cgcatcaagg
    38641 caaagttgtt gtttaatcgc cggattaaca aaaatgcgtg tgacgtcttt gtcctgggcg
    38701 gcgagtttga tcaagctgaa aatttctggc ttccacagcg tggagacaac gtgtttaccg
    38761 tcgcgggaaa ctaagtccag tgcttgcggg cgcaagagct gcgcggaggt ccagcgagtt
    38821 ttcggcagtt gcagaaagat atcgacatcc agtccggtct ggtggctggc atgaccgccg
    38881 ttgaaacgcc caccagcggg catccccata tcgccaatca gcaccgtacc catgcccaga
    38941 ttgctcacct ggctactcag acgctggata aacatcacca gatccgggtg accgaaatag
    39001 cgacgctgat cggtacgcat gacctgataa tgttcggact gtatcggcag cgtgtcagcg
    39061 ccgacgatac agccattaga aaaactgcct atcgattgtg cgctacccgg cacaggttgg
    39121 gttatttttt gccacggcgt cgctgccagg ctagcgctac tggcaagcag agccagcagc
    39181 gcaatcgcgg ttttgttcat tttttaccag cgtggaatat cagtcttcac atcggcattt
    39241 tgcgcccgct gccgtaacag gtgatccatt aaaacgatcg ccagcatcgc ttcagcgatc
    39301 ggcactgcac ggatcccgac acagggatcg tgacggcctt tggtgatcat ctcaacttct
    39361 tcgccaaagc ggttaatagt gcgccccggc acggtaatgc tggaggtcgg tttcagcgcc
    39421 atatgagcaa tgatttgctg cccgctgctg ataccgccga gaatgccgcc cgcatggttg
    39481 ctctggaaac cgtctttggt gatttcgtcg cggttctggc tgccgcgcag cgccactacg
    39541 tcaaaaccgt cgccaatttc cacgcctttc accgcgttga tgctcatcag cgcatgggcg
    39601 atgtcggcat ccaggcggtc aaagaccggc tcgccaagtc cggcggggac accactggca
    39661 acaacggtga ctttcgcgcc gatggagtcg ccctcttttt tcagcgcgcg catcagttca
    39721 tctaacgcgt cgattttgtc ggggtccggg cagaagaacg gattttgttc aaccagcgac
    39781 cagtctttga tttccagcgg aatgtcgccc atctgggtca agcagccgcg gatttcaata
    39841 ccaaatttct cggcgagata ttttttggca atcgcccctg ccgccacgcg catggcggtt
    39901 tcgcgggcgg aagaacgtcc accgccgcga taatcgcgca gaccgtattt ttgttcgtag
    39961 gtgtaatcag catggcctgg gcggaaaacg tctttaattg cgctgtaatc ctgagagcgc
    40021 tggtcagtgt tttcgatcaa caagccaatg ctggtgccag tagtaacgcc ttcaaaaaca
    40081 ccggagagaa ttttgacctg atccggctcg cggcgctggg tggtatagcg cgatgtccca
    40141 gggcgacgac ggtcgaggtc atgttgcagg tccgcttccg tcagcggaat gcctggcgga
    40201 acaccatcga cgatgcagcc gagcgccagc ccatgcgatt cgccgaaggt ggttacgcga
    40261 aagagttgtc caattgtgtt tccagccatc acggctccgt tattgttgtg tttgcgtgtt
    40321 tacttaatct ttataaatcg cgaaatattc acgggccgcc agcagctgct ctttggtgag
    40381 cataaacaca ccatcgccgc cgttatcaaa ctccagccag gtgaacggaa catccggata
    40441 ttgttccata agatgtacca tgctgttgcc gacttcacaa atcaacacgc catcatcagc
    40501 aaggtaatct gccgcgtttc caagaatgcg acgtgtcagt ttaaggccgt cggtgccaga
    40561 tgccaggccc agttccggct cgtgacggta ttcgtttggc aggtcggaca tatcttccgc
    40621 gtcgacatac ggcgggttag tgacaatcag atcgtattgc actttcggca aatcacggaa
    40681 cagatcggaa cgaatcggaa tgacgttgtg gatcagaccg tgttcttcga tgttctgttc
    40741 ggcaaccgcc agcgcgtcgg gagagatatc taccgcgtcg acttccgctt ccgggaaggc
    40801 ataagcacag gcaatggcga tgcagccgct accggtacac atatctaaaa tatgctgcgg
    40861 ttgcttgctg atgagtccgg caaatttatt gttgatcagt tcaccaatcg gcgagcgcgg
    40921 caccagcacg cgttcatcga cgtaaaattc atgaccgcag aaccaggctt tgttggtcag
    40981 gtaagccacc ggaatgcgct cgttgacgcg gcggatcacg cgttcaacaa tacggtgttt
    41041 ttcgctggag gtcagacgcg cggtgcgcat atcttccggg atatccagcg gcaggtagag
    41101 cgatggtaac accagctgta cggcttcatc ccacgggtta tcggtgccgt gaccgtacca
    41161 gatatttgcc gcgctgaagc ggcttaccga ccagcgcaac atgtcctgaa tggtttgcag
    41221 ctcatttact gcttcatcaa cgaaaatttt atccacgtat tcctccaggg catgatcgca
    41281 ataatttcgg cggctagttt gccatgaaga tgacgataaa tcagcattca cgcgcggtga
    41341 gtgaggaaaa atacgtttaa aacgatcgat tgtgctacga gtcgggtaaa ctgtaggaaa
    41401 attagaaata gagacagata aatgaaaaag aaaacaacac ttagcgagga ggaccaggct
    41461 ctgtttcgcc agttgatggc ggggactcgc aagattaagc aggacacgat tgtccaccga
    41521 ccgcagcgta aaaaaatcag cgaagtgccg gtgaaacgct tgatccagga gcaggctgat
    41581 gccagccatt atttctccga tgagtttcag ccgttattaa ataccgaagg tccggtgaaa
    41641 tatgttcgcc cggatgtcag ccattttgag gcgaagaaac tgcgccgcgg cgattattca
    41701 ccggaattgt ttttggattt acacggtctg acgcagctgc aggccaagca ggaactgggc
    41761 gcgttgattg ccgcctgtcg ccgcgaacat gtgttttgtg cctgcgtgat gcatgggcac
    41821 gggaagcata ttttgaagca acaaacaccg ctgtggctgg cacaacatcc gcatgtgatg
    41881 gcatttcacc aggcaccgaa agagtacggc ggtgatgcgg ctttgttggt gttgattgaa
    41941 gtggaagagt ggttgccgcc ggagttgccc tgaggagttg agcgtgttgt gccaggagcg
    42001 cggaatggca ggggcgaatg accctgccag gtgagaaaag gtgcgctaat acatattcag
    42061 tatgtaagtg aatcggcagg tagaatctat gggctgtcga gatgtcgtgt ccacgcgtag
    42121 cttcatatca tgcagttgcc tggacgtaat cggttgcggc tcaggcgtca ccgtcacttt
    42181 ggcattatca cagccggaag tcgagaaggt catctgctgt gcaataaaac ggcagtaggc
    42241 accacgctca tcaaaattat tcccgttaca cagtttgcca ttttccatca ggaaagtggg
    42301 cagtgtttgc tcaataacac cacctgttga ctgcaacctg acgactttgt tatgtacaac
    42361 ataattttca ttccaggaaa catcatacgg tgccaggggc accgccggac atgaagaact
    42421 caccttcgtt gggccaatat agtcaggaaa atcatcatca tacgttttag cgaagggatc
    42481 tctactctta acaaacgacg acacaatcca cggacaacca tgtaaattcc catcgtaata
    42541 aacaaacatc tttaattcat tacatgtcca tcccgttttg gtctggctat catactgctc
    42601 taatccagga caggctgaca tggataccct gctgtccgaa attttcgaca tagatggttg
    42661 agtagaacta tcgccagtcc acgaaactaa atccggctgt gaattgggca tgaatgtagc
    42721 cttaaatata gcgtaattaa ctgaatctgg cgtcgctata atcagatgaa actcaaactc
    42781 tgtcgcaggt gaactggcac cagctggcac atataactca ccaactttcg tcgccgcctg
    42841 gccgcatgta ttgaacatta acagcaataa aagacataaa acatgacgaa ggattttcat
    42901 tttaaatata ctcaaaagtt gctgtcaggc tggcggtgaa atcaccgatg gttacatcac
    42961 gaccgctttt cgcctggatc caggcccgga aattaatatc attgttgccg ttggtaagcg
    43021 caaaggtcgc cccggtggtg ttgttaatat ccacccgctt gccgtcgagc gtttccatcc
    43081 ctatccccac gccctgcgcg gtggaggtgt catcgactgc cagaaaaccc ggctgaccgc
    43141 tgtcttcggt gccgctcagc gtgactttga cgttgtatcc cgccggccct ttgcagtctt
    43201 tcagcttaaa cacaaccggc agatgcgcgg actgcccaga taccatcaaa tcccggttgc
    43261 tgacggtggg aaaatggatt tctgccaggt tggcaccatc caccaccagc gtgcaggatt
    43321 tactcagcag gttgccgtaa aaatgcaggt tgttgtcgac tgcctgagct cctgtgctgg
    43381 cggtcagcag caggcctgct aacagcgcat aaccgggtga ataaatgtgt gctgttttca
    43441 ttggtaatcc acccgtaagg ttgcgtaagc agaaaaggtc gtgtccgtca gcaccgcatc
    43501 agccgctttg accggtaccg cctccagctt tggcttctgg ctaaaatcgg tggcgttaat
    43561 ggaaagcggc tcgttgagct taaaacgctg accgtcatgc tgaagctcaa ttccgaagcc
    43621 cggtacatcc gtctcaatag cagagtcgtt gtaggaggtg gcagtccccg tccaggagag
    43681 tgtcatattc cactcgggat cgcggacatc cgagtcgcag ctcagttcgt agtccacttt
    43741 ctgacgacca taattgccat caatgctgtc gataatgaga tcgcgaaatt gcacttcgat
    43801 ggtttgcccg ccgctgatgc tacaggcggg cggcgacatc agcgtaccgt ggaatgtgat
    43861 gtcatcttcc gccgccagca cagccccgca atcagcgcac agtaaaagga gcaggaattt
    43921 tttcattggt aatccaccac catggtcatc gtggcgttaa actccgacgg cgtcagttgc
    43981 accccgctct gttttaccgg caccgctttc agctgaggct gagtgttgac attaaaaggc
    44041 gtccagttat tttcaccaac tgcaaacagg gagttgtcgg cggcgttttc tatccgcacc
    44101 cccaggccgg cgatatccgt gctgagaacc gtttctccgt tgatcacggc ggtattgcct
    44161 ttgagctgca tccgtaagtc atcgctgact ttattaccgc agttgagggt gtatttcgca
    44221 tcctgttggt aattggtacc atcgatcctg ttgattatca cattgccaaa tttcacagcg
    44281 cttcccttca cggagcaagg cggcggtcca tcgaccagga ttcgcagcgt cagatccacc
    44341 tgtttgatat cactgccagc aacagccaaa ccgctgatgc cccatccggc cagcaccagc
    44401 aaccctgcgc ccgctacaaa cttgtgactc ataagtccct cccggcatca gcctttgcgg
    44461 ctctgctctt catccacctg gcagctgttg ccggtgcata tgaagtacag cggcatccgc
    44521 gcgccgtagt cgttgatgta agtcagcacc ggcacgctgt ccatcttcgc attcagcggt
    44581 gcggaggttt tcggctccat catcagcggt gtgaatcccg ccggcgggtt gccgcctttt
    44641 ttagcgctgg cgttgctgat gatcacgtag tacggcgtcg ggttattgac ggtgaagtcc
    44701 tggccgttac ggatgagcgt caccttatgc tgccacggac ttttcatgct ggtgttctcc
    44761 agcgccttcg gtcgccagaa cagtttgata cgcgtctgca gcgcaatctg cagtgtgttc
    44821 ggtttatttg atttcggcgg aatttcgcgc acgttgaaat agaacacgct ctcgcggtcg
    44881 gccggcagtt tgttgatatc cggcagcccc tgcaccttca cctggccgtt catcatcgca
    44941 tcgatgcgct gcactggcgg aagcaccgca agcggcgagc tgattttgtt gccctttgca
    45001 tcctctatcc agctctgcgc cagataaggc agtgtgggat cgttgttgcg cagcgtgacg
    45061 ctgatggact tgtcgctttc gttaaacacc agccgtgtcc tgtcgggcgt caccgaagcg
    45121 tggctggaga aactgaccgc tgtcgccagc agcgccagtg ccgggacaac gatttttgca
    45181 gtgttagtca gattaaacat ggatcattcc ttaatcacgt tactggttta ctgaaattga
    45241 agtcggaggt tccgttggcg tcacccgctg tgtctgttcc tgaatcgccg gtttgaccgc
    45301 cggcgaagtg ataggtgcta cattgccttt atgctggcat ggcagtaaca agccgttaaa
    45361 cagatcggca ggcagcgggt ccggcaggtt gatatcgcaa tgcgcaacac cactccagaa
    45421 cacactcatg tgttcgccag gtttcacccc agccagataa acattgccgt catcatcgac
    45481 aaggcccact gtctgctcgt tatcattttt tacttctgcg ccaaacggag gatggctgcc
    45541 gtcttgtaaa cgcagcaccg ccatcgcttt ttgaccactg atgacggcaa atttgcggta
    45601 gccaatcgcc ccttcagtta gcgtggcttg taccaccgac tgggttgctt cagcgttttc
    45661 aggcaatctg ttgaggtcga tatacgcctg attgcggtaa tagttattga cgtcagaaac
    45721 gacggcttta ccaaacatat tggtataaac agcagcaccg ttaccttcta ccggaacatc
    45781 ggcaacgcca tcggcatcaa tcaacaagcg tgtcccgccc atattctggg tacggtgaag
    45841 tgcgccaccg tgggcagtaa gcgttgcgcc gccctgtaac gacaagcccg cagaggtgta
    45901 ttgcccttca tggtagttcg cactgaggtc aacctgcgcc agcgaaccat catggctgta
    45961 atagccgtca acgctggtgt gtttgtcact ggtgccgacg ttcaactgat agtgagtcgc
    46021 gtcatcgaca cggctgaaat aaccgacctg actgctgtcc gtcccactgc catagttacc
    46081 gttatagcta acggtactgt tgtcgcccca cggcatactg agcgaaatgt acatgccttt
    46141 gtcggcctgg ttgtcatact cgtaacggta gccagtcatc gagatgctga cattacgaat
    46201 actgcccata ttgaaatagt gcgagagcat gatgttgtag tttgtctgct cctcgcgatc
    46261 ccagtaggta tggcgggtat agttgagata aaccgaaaca cccgcatcgc ggaagttctg
    46321 gttataagtg gcggtgtaca tctctttgtc gttgcccgtg cgtaccattc cgctgtcact
    46381 ggcatccaga tactcgctca tggtcataaa gttctcttcc gagaagcgat atccagcaaa
    46441 agtaacgcgg ctgttgagct ggtcaaagtc tttggaataa ctcacacgga aggagttacc
    46501 gtccagcgaa cctttgccat aagcggtgtc tttatccagt ttggtgtgcg agtgagtaac
    46561 atcaaacgcg accgcgccga atgtagacaa atcgcgaccg acgccaagcg ccgcagactg
    46621 atagttttca tctcccagtg cgccgccata taacgaccag ccgttagcga tcccccagga
    46681 agcttcagca ccagaaaaaa attcaccctc gacatggtgt ccccactctt gcggacggcc
    46741 catcatgatc ttatagcgaa cctgacctgg gcgagtgagg tatggcatcg aggcggtgct
    46801 gatgtcatat tcctgcacct ggccgttctg ttcttcaatg cgaatatgca acgtaccgga
    46861 gacagaatca ccaagatcct gaatacgaaa cggtccagcc ggaacctgcg tttcgtaaat
    46921 cacacgcccc atctggctga cggtcacttt tgctgtggtg tgtgccacgc cggaaatgtc
    46981 tggcgcgtag ccgcgcagat tgggaggcaa catttgatcg tcagtactga cactgccacc
    47041 aacatagtta aaaccatcaa aaatatcgga tttgaggtaa tcctcgccca gcgccagttt
    47101 ggcttttaat gatggtaacg cccgccaggc atagtagcga ctccactccc attttttttg
    47161 agtttcatcg ccgctgaatt catcgtcatc atcattactg cgagtatgtt gatagttagt
    47221 ctgccagtca gcacgcatac gccacggccc caggttaacc ccgaccgtcc cgttgccgct
    47281 gatctcgtta ctatcatcac cgccattttc ttcatgccgg gtttgtgcgt tgatgctgta
    47341 gtccgcgacg atcccggaga tgccgtcatc ccaacgtgaa ggcggatccc agtcgggata
    47401 agtatattcg aggtaagcct gtggcagtga aatgactaat gcggactggc ttaaatcagc
    47461 cttaatttcc atgccttcca gttgaccgga tttcaggcat ttagcatcgt ggctccattg
    47521 cagatttttc gccacgtctt cttttaaacc aaactgcgct accagttccg gtgtcagaca
    47581 agcatagctt ttgctcgcgt catcttcacc agcataccag taaatatcgt actcttccgc
    47641 caatggctgt ttatttagtt gaacctgtaa attgtatttt ccgggctcaa catatccctg
    47701 gctggaaaaa cgcttcagat caatttttgt gtcgcctttt aattccagaa aacgggaatc
    47761 aaactgaatg tcgtcttcag cccagacact gctataactc cctgacattg ccagcgcaat
    47821 gcaccacgga agaatccgta atcgaaaaag agaatggtca ggcatacacg tttctatccg
    47881 tttttattct tgttcggtca aaatccattt aaatccccca tagcagtgtc gctatggggg
    47941 tatgccttac aggtaagtaa tctggaaggt ggtatttgct tcgaaattac ccagatctgg
    48001 cgcatcagct gcgccgacca gccaggcttt aaagttcagt gtctgcttcg ctttgccttt
    48061 attggtagaa gtgtctttca ctattttctg gtcaataccc atgccgcttt tgtaagaggt
    48121 acccagagag tcaccgatcg ccaggctaac attgttaaat gccgcaccgg tatcggtatt
    48181 gaaaatggtg taataattgc cgctatttgc agaagaaacg gtaccagtaa aggtagtggt
    48241 cattgtttcc tgagtatcaa atacgcaatc ttgcagacga atctggaaac ttttcggcgt
    48301 ggtggtgccg ttatttttca gtttttcggc accgatatca cccatatcaa cttccagttt
    48361 gtgatcgtcc ggaacgatag aacacactga gtcttcaatg gtgccgtaaa aacgtgcagt
    48421 accgttgtta cccgcagcga ttgttgccgt agaggtgaaa acgcccatca ccattgccgc
    48481 agcaatagct gttttaacaa acttactcat tatttgtcct taaactataa ataattaaaa
    48541 atataaacat tacaatacat tgattcagtc aatacccaat gtttataccc agccattgaa
    48601 tacggcatgc gatccaaaaa gaacaggtcg tagaaaattg tattctttgg tgtggggccg
    48661 catagtgatg ttttcacccc cctgatgcaa tttaatttta ctaaagagta aatgtagatt
    48721 taatta
//
LOCUS       DAZFQD010000017        47610 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.017, whole genome shotgun sequence.
ACCESSION   DAZFQD010000017 DAZFQD010000000
VERSION     DAZFQD010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 47610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47610
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.017"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            49..681
                     /gene="comR"
                     /locus_tag="ACHKTW_001098"
     CDS             49..681
                     /gene="comR"
                     /locus_tag="ACHKTW_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HFO6462162.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(743..1282)
                     /locus_tag="ACHKTW_001099"
     CDS             complement(743..1282)
                     /locus_tag="ACHKTW_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HFO6462163.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(1492..2796)
                     /gene="ndh"
                     /locus_tag="ACHKTW_001100"
     CDS             complement(1492..2796)
                     /gene="ndh"
                     /locus_tag="ACHKTW_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HFO6462164.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAVKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(3204..3746)
                     /gene="ycfP"
                     /locus_tag="ACHKTW_001101"
     CDS             complement(3204..3746)
                     /gene="ycfP"
                     /locus_tag="ACHKTW_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase YcfP"
                     /protein_id="HFO6462165.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(3769..4794)
                     /gene="nagZ"
                     /locus_tag="ACHKTW_001102"
     CDS             complement(3769..4794)
                     /gene="nagZ"
                     /locus_tag="ACHKTW_001102"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HFO6462166.1"
                     /translation="MGPVMLDVKGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALSGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPQKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSPYWLKTVLRQELGFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISTRLNQLHERW
                     QEEKAGH"
     gene            complement(4805..5629)
                     /gene="thiK"
                     /locus_tag="ACHKTW_001103"
     CDS             complement(4805..5629)
                     /gene="thiK"
                     /locus_tag="ACHKTW_001103"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019165 - thiamine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HFO6462167.1"
                     /translation="MPFRSNNPIMRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPASIAPKPHLYLRDWMVVDYLPGAVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKAGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(5610..6251)
                     /gene="lpoB"
                     /locus_tag="ACHKTW_001104"
     CDS             complement(5610..6251)
                     /gene="lpoB"
                     /locus_tag="ACHKTW_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HFO6462168.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDQTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(6265..6642)
                     /locus_tag="ACHKTW_001105"
     CDS             complement(6265..6642)
                     /locus_tag="ACHKTW_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HFO6462169.1"
                     /translation="MRKGCFGLVSLALLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVSIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(6645..7004)
                     /gene="hinT"
                     /locus_tag="ACHKTW_001106"
     CDS             complement(6645..7004)
                     /gene="hinT"
                     /locus_tag="ACHKTW_001106"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HFO6462170.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            7338..9527
                     /gene="fhuE"
                     /locus_tag="ACHKTW_001107"
                     /pseudo
     CDS             7338..9527
                     /gene="fhuE"
                     /locus_tag="ACHKTW_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
     gene            complement(9587..11020)
                     /gene="ptsG"
                     /locus_tag="ACHKTW_001108"
     CDS             complement(9587..11020)
                     /gene="ptsG"
                     /locus_tag="ACHKTW_001108"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005355 - glucose transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:1904659 - glucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HFO6462171.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFSFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLFPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(11315..12112)
                     /locus_tag="ACHKTW_001109"
     CDS             complement(11315..12112)
                     /locus_tag="ACHKTW_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFO6462172.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDVSRLQSIR"
     gene            complement(12123..13127)
                     /gene="holB"
                     /locus_tag="ACHKTW_001110"
     CDS             complement(12123..13127)
                     /gene="holB"
                     /locus_tag="ACHKTW_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFO6462173.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNMLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVAE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(13124..13765)
                     /gene="tmk"
                     /locus_tag="ACHKTW_001111"
     CDS             complement(13124..13765)
                     /gene="tmk"
                     /locus_tag="ACHKTW_001111"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFO6462174.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(13755..14777)
                     /gene="yceG"
                     /locus_tag="ACHKTW_001112"
     CDS             complement(13755..14777)
                     /gene="yceG"
                     /locus_tag="ACHKTW_001112"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HFO6462175.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTWMYTANTTDVALLKQAHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(14780..15589)
                     /gene="pabC"
                     /locus_tag="ACHKTW_001113"
     CDS             complement(14780..15589)
                     /gene="pabC"
                     /locus_tag="ACHKTW_001113"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008696 - 4-amino-4-deoxychorismate lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HFO6462176.1"
                     /translation="MFLINGYKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIQ
                     RLQDACQRLMISCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTAYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVSFSSATLYEYLAPLCERPN"
     gene            complement(15709..16950)
                     /gene="fabF"
                     /locus_tag="ACHKTW_001114"
     CDS             complement(15709..16950)
                     /gene="fabF"
                     /locus_tag="ACHKTW_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFO6462177.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(17038..17274)
                     /gene="acpP"
                     /locus_tag="ACHKTW_001115"
     CDS             complement(17038..17274)
                     /gene="acpP"
                     /locus_tag="ACHKTW_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFO6462178.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(17485..18219)
                     /gene="fabG"
                     /locus_tag="ACHKTW_001116"
     CDS             complement(17485..18219)
                     /gene="fabG"
                     /locus_tag="ACHKTW_001116"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HFO6462179.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(18232..19161)
                     /gene="fabD"
                     /locus_tag="ACHKTW_001117"
     CDS             complement(18232..19161)
                     /gene="fabD"
                     /locus_tag="ACHKTW_001117"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFO6462180.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGPAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDASIAKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVPVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(19177..20130)
                     /gene="fabH"
                     /locus_tag="ACHKTW_001118"
     CDS             complement(19177..20130)
                     /gene="fabH"
                     /locus_tag="ACHKTW_001118"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFO6462181.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLGIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(20198..21268)
                     /gene="plsX"
                     /locus_tag="ACHKTW_001119"
     CDS             complement(20198..21268)
                     /gene="plsX"
                     /locus_tag="ACHKTW_001119"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFO6462182.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRMALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(21349..21522)
                     /gene="rpmF"
                     /locus_tag="ACHKTW_001120"
     CDS             complement(21349..21522)
                     /gene="rpmF"
                     /locus_tag="ACHKTW_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFO6462183.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(21574..22095)
                     /gene="yceD"
                     /locus_tag="ACHKTW_001121"
     CDS             complement(21574..22095)
                     /gene="yceD"
                     /locus_tag="ACHKTW_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HFO6462184.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEAQK
                     PNPFAVLASLKRK"
     gene            22294..22878
                     /gene="yceF"
                     /locus_tag="ACHKTW_001122"
     CDS             22294..22878
                     /gene="yceF"
                     /locus_tag="ACHKTW_001122"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HFO6462185.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(22990..23949)
                     /gene="rluC"
                     /locus_tag="ACHKTW_001123"
     CDS             complement(22990..23949)
                     /gene="rluC"
                     /locus_tag="ACHKTW_001123"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HFO6462186.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            24522..27707
                     /gene="rne"
                     /locus_tag="ACHKTW_001124"
     CDS             24522..27707
                     /gene="rne"
                     /locus_tag="ACHKTW_001124"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008995 - ribonuclease E activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HFO6462187.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAAPVVAPAPKAAPATPATPA
                     QPGLLSRFFGALKALFSGSEETKPTEQPAPKAEAKPERQQDRRKPRQNNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQAPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETAAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTEVPVAAAVEPVVSAPVVEEMAEVVEAPVPVAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPVVEPQEETADIEEVAETAEVVVAEPE
                     VVAQPAAPVVAEVAAEVETVAAVEPEITVEHNHATAPMTRAPAPEYVPEAPRHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(27904..28857)
                     /gene="flgL"
                     /locus_tag="ACHKTW_001125"
     CDS             complement(27904..28857)
                     /gene="flgL"
                     /locus_tag="ACHKTW_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HFO6462188.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFATQKVSLEESVLSQVTTAIQNAQEKIVYASNGTL
                     SDDDRASLATDIQGLRDQLLNLANTTDGNGRYIFAGYKTETAPFSEEKGKYVGGAESI
                     KQQVDASRSMVIGHTGDKIFDSITSNAVAEPDGSASETNLFAMLDSAIAALKTPVADS
                     EADKETAAAALDKTNRGLKNSLNNVLTVRAELGTQLNELESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(28869..30512)
                     /gene="flgK"
                     /locus_tag="ACHKTW_001126"
     CDS             complement(28869..30512)
                     /gene="flgK"
                     /locus_tag="ACHKTW_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HFO6462189.1"
                     /translation="MSSLINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTIMAQA
                     NSTLGAGGWVGNGVYVSGVQREYDAFITNQLRAAQTQSSGLTARYEQMSKIDNMLSTS
                     TSSLATQMQDFFTSLQTLVSNAEDPAARQALIGKSEGLVNQFKTTDQYLRDQDKQVNI
                     AIGASVDQINNYAKQIASLNDQISRLTGVGAGASPNNLLDQRDQLVSELNQIVGVEVS
                     VQDGGTYNITMANGYSLVQGSTARQLAAVPSSADPSRTTVAYVDGTAGNIEIPEKLLN
                     TGSLGGILTFRSQDLDQTRNTLGQLALAFAEAFNSQHKAGFDANGDAGEDFFTIGKPA
                     VLQNTKNKGDVAIGATVTDASVVLATDYKISFDNNQWQVTRLASNTTFTATPDANGKV
                     AFDGLELTFTGTPAVNDSFTLKPVSDAIVNMDVLITDEAKIAMASEEDAGDSDNRNGQ
                     ALLDLQSNSKTVGGAKSFNDAYASLVSDIGNKTATLKTSSTTQGNVVTQLSNQQQSIS
                     GVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINIR"
     gene            complement(30578..31519)
                     /gene="flgJ"
                     /locus_tag="ACHKTW_001127"
     CDS             complement(30578..31519)
                     /gene="flgJ"
                     /locus_tag="ACHKTW_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HFO6462190.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKAGEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAVPMKFPLETVVRYQNQALSQLVQKAVPRNYDDSLPGDSKAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGPVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQALQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(31519..32616)
                     /gene="flgI"
                     /locus_tag="ACHKTW_001128"
     CDS             complement(31519..32616)
                     /gene="flgI"
                     /locus_tag="ACHKTW_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HFO6462191.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTASLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(32628..33326)
                     /gene="flgH"
                     /locus_tag="ACHKTW_001129"
     CDS             complement(32628..33326)
                     /gene="flgH"
                     /locus_tag="ACHKTW_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HFO6462192.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(33379..34161)
                     /gene="flgG"
                     /locus_tag="ACHKTW_001130"
     CDS             complement(33379..34161)
                     /gene="flgG"
                     /locus_tag="ACHKTW_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HFO6462193.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYTRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL"
     gene            complement(34299..35054)
                     /gene="flgF"
                     /locus_tag="ACHKTW_001131"
     CDS             complement(34299..35054)
                     /gene="flgF"
                     /locus_tag="ACHKTW_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HFO6462194.1"
                     /translation="MDHAIYTAMGAASQTLNQQAVTASNLANASTPGFRAQLNALRAV
                     PVEGLSLPTRTLVTASTPGADMTPGKMDYTSRPLDVALQQDGWLAVQSADGSEGYTRN
                     GSIQVDPTGQLTIQGHPVIGEAGPIAVPEGAEITIAADGTISALNPGDPANTVAPVGR
                     LKLVKATGSEVQRGDDGIFRLSAESQATRGPILQADPTLRVMSGVLEGSNVNAVAAMS
                     DMIASARRFEMQMKVISSVDDNAGRANQLLSMS"
     gene            complement(35074..36279)
                     /gene="flgE"
                     /locus_tag="ACHKTW_001132"
     CDS             complement(35074..36279)
                     /gene="flgE"
                     /locus_tag="ACHKTW_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HFO6462195.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLVNMQGLQLTGYPATGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPTVTPFSASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     TGTAEPAMKLVFNANGVLTSNPTENITTGAINGADPATFSLSFLNSMQQNTGANNIVA
                     TTQNGYKPGDLVSYQINDDGTVVGNYSNEQTQLLGQIVLANFANNEGLASEGDNVWSA
                     TQSSSVALLGTAGTGNFGTLTNGALEASNVDLSKELVNMIVAQRNYQSNAQTIKTQDQ
                     ILNTLVNLR"
     gene            complement(36304..36999)
                     /gene="flgD"
                     /locus_tag="ACHKTW_001133"
     CDS             complement(36304..36999)
                     /gene="flgD"
                     /locus_tag="ACHKTW_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HFO6462196.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGTLTDGSTAPNGSYNVAISASNGGTQLVAQPLQFALVQGVIRGNRGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(37011..37415)
                     /gene="flgC"
                     /locus_tag="ACHKTW_001134"
     CDS             complement(37011..37415)
                     /gene="flgC"
                     /locus_tag="ACHKTW_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HFO6462197.1"
                     /translation="MALLNIFDIAGSALTAQSQRLNVAASNLANADSVTGPDGQPYRA
                     KQVVFQVNAAPGAATGGVKVADVIESQAPDKLVYEPGNPLADAKGYVKMPNVDVVGEM
                     VNTMSASRSYQANVEVLNTVKSMMLKTLTLGQ"
     gene            complement(37419..37835)
                     /gene="flgB"
                     /locus_tag="ACHKTW_001135"
     CDS             complement(37419..37835)
                     /gene="flgB"
                     /locus_tag="ACHKTW_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HFO6462198.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            37990..38649
                     /gene="flgA"
                     /locus_tag="ACHKTW_001136"
     CDS             37990..38649
                     /gene="flgA"
                     /locus_tag="ACHKTW_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HFO6462199.1"
                     /translation="MLTIKRSVAIIAILFSPLSTASNLTSQLHNFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRLDTLPPRTVLDINQLVDAISLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMVSGQVVSGVVDADGNILIN
                     L"
     gene            38725..39018
                     /gene="flgM"
                     /locus_tag="ACHKTW_001137"
     CDS             38725..39018
                     /gene="flgM"
                     /locus_tag="ACHKTW_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HFO6462200.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDAPVTNSRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            39023..39439
                     /gene="flgN"
                     /locus_tag="ACHKTW_001138"
     CDS             39023..39439
                     /gene="flgN"
                     /locus_tag="ACHKTW_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HFO6462201.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRKKEPNTANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTTHRGGKKISI"
     gene            complement(39479..41014)
                     /gene="murJ"
                     /locus_tag="ACHKTW_001139"
     CDS             complement(39479..41014)
                     /gene="murJ"
                     /locus_tag="ACHKTW_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HFO6462202.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(41124..42047)
                     /locus_tag="ACHKTW_001140"
     CDS             complement(41124..42047)
                     /locus_tag="ACHKTW_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFO6462203.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWMLQGAWSPTRAKALP
                     ICESWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSAGPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITDMREWREERGQGVVHKPIPGWQSTLEQRGFVGCARHFIEC
                     VQNQTVPQTAGEQAVLAQRIVDKIWRDAMSE"
     gene            complement(42049..42696)
                     /locus_tag="ACHKTW_001141"
     CDS             complement(42049..42696)
                     /locus_tag="ACHKTW_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HFO6462204.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAEVESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPAVTDMSNAVDGDLQARVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(42707..43291)
                     /gene="rimJ"
                     /locus_tag="ACHKTW_001142"
     CDS             complement(42707..43291)
                     /gene="rimJ"
                     /locus_tag="ACHKTW_001142"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HFO6462205.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            43527..44735
                     /gene="mdtH"
                     /locus_tag="ACHKTW_001143"
     CDS             43527..44735
                     /gene="mdtH"
                     /locus_tag="ACHKTW_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HFO6462206.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSAGAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVHTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            44799..45446
                     /gene="grxB"
                     /locus_tag="ACHKTW_001144"
     CDS             44799..45446
                     /gene="grxB"
                     /locus_tag="ACHKTW_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HFO6462207.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVADYRDNMAKQTQINLLSSMAI"
     gene            45580..46139
                     /locus_tag="ACHKTW_001145"
                     /pseudo
     CDS             45580..46139
                     /locus_tag="ACHKTW_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
     gene            46245..47291
                     /gene="pyrC"
                     /locus_tag="ACHKTW_001146"
     CDS             46245..47291
                     /gene="pyrC"
                     /locus_tag="ACHKTW_001146"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0019856 - pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFO6462208.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHNFTPLMTCYLTDSLDPNELERGFNEGVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            47365..47610
                     /gene="dinI"
                     /locus_tag="ACHKTW_001147"
     CDS             47365..47610
                     /gene="dinI"
                     /locus_tag="ACHKTW_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HFO6462209.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
BASE COUNT        11506 a        12875 c        11850 g        11379 t
ORIGIN      
        1 tatttttata ataacctttc attcacagtc accaggtaca acggcatcat ggcaactgac
       61 tcaacacaat gtgtaaaaaa aagccgtggc cgcccaaaag tgttcgacag ggatgccgcg
      121 cttgataagg ccatgaaatt gttctggcaa cacggttatg aagcgacttc tcttgcggac
      181 ctcgtcgaag cgaccggagc caaagcgccc acgctatacg cggaatttac caacaaagag
      241 gggttatttc gtgccgttct cgaccgctat atcgatcgtt ttgccgctaa gcatgaagca
      301 cagctgtttt gtgaagagaa aagcgtggag tctgcgctgg ctgactattt tgctgccatc
      361 gccaactgct ttaccagcaa agacaccccg gcaggctgct tcatgatcaa caactgcacc
      421 accctctccc cagattcagg agatatcgcc aatacgttga aatcacgcca tgcgatgcaa
      481 gagcgcactt tgcagcagtt tttatgtcaa cgacaagcgc gcggggaaat cccgacccac
      541 tgtgacgtga cacatctggc agaattcctt aattgtatta ttcaggggat gtcgatcagc
      601 gcacgcgaag gtgcatcgct ggaaaaacta atgcagattg ccggaacgac tttgcgttta
      661 tggcccgaac tggtgaaata agtcgcaaaa aaagcccctc agcctgagcg aatgaggggc
      721 caaattgcag agtacagcct tgttatactt tgttattcaa aatcagttgc ccattactat
      781 ctagcgggat ctgcgtaccc ggatcgcggt ccatgcggat tttgccctgc tgatcgccaa
      841 tcttataggt cacatcataa ccgagcattt tttctgactt gtcatacacc gttttacaac
      901 gctgttgcgt agtcgtgtaa gtatcgcttt cctggagaga gccctggatc tggttaccgg
      961 catatccacc acccagcgcc cccacaacag tggcgacatc tttaccgcga ccaccgccaa
     1021 actgatgccc tatcacgccg ccagcaacag cgccgagcac cgacccggta atgcgatttt
     1081 catcctgcac cggtcgacga tgggtcactg tgacgttgcg acactcctga cgcggtgttt
     1141 taaccgtttc cttgattggg gttgcagaaa caacctgagc gtattgcggg ccacgttcaa
     1201 acacgttcag actggccact gccgctacgc ccagcgcagc tgcgacacca atcccgatac
     1261 ccgccaacat tgatttattc acgggaacac ctccttcttt gcagttatgc caatcatgca
     1321 agcgaaggag aaaagcagct atcagacaga agatgaaaag cgcgggaatg gctggaaata
     1381 cttaaggatt cggaggagtc tgaaggaatg cctgatgcga cgcggctgcc gatcgcgctg
     1441 ttcctcaagg gcggataagg cgtttatgcc acatccgcca gtgtacgtcg attaatgcaa
     1501 cttcaaacgc ggacggataa cgcggttaat actccccacc agcatcatta atccggtttt
     1561 aaagtaacca tgcagcgcaa tctgatgcat tcggtatagc gagatatata caaagcgcgc
     1621 aattcgtcct tcaatcatca ttgagccgcg cgtcaggtta cccatcaggc taccaacggt
     1681 ggagaagttc gacagcgata ccagcgaacc atgatcttta tactgataat ttttcagcgg
     1741 cttaccattc atctgcgcca gaatgttgtt cattgcgcaa gtcgccatct ggtgtgcagc
     1801 ctgagcacgc ggcggaacaa agcccccttc cggacgcggg catgacgcgc agtcgccaat
     1861 agcgtaaatg tctggatcgc gggtggtttg cagcgtcggt tccaccacca gctggttgat
     1921 acggttagtt tcaagaccac cgatatcttt caggaagtct ggcgctttga tcccggctgc
     1981 ccacaccatc agatcagcct caatatattc gccatcttta gtgtgcaggc cgccttcatc
     2041 agcactggtg accatggttt gcgtcagcac gcgaacgcca agtttcgtta gctcgttgtg
     2101 ggccgcagca gagatacgtg gcggtaatgc aggcaaaata cgttctcccg cttctaccag
     2161 cgttacgttc agggcttcgt tggtcaggcc tttgtaaccg tagctgtgca gttgcttgac
     2221 cgcgttgtgc aattcagcgg agagttctac acccgtcgcg ccgccgccga caatcgcaat
     2281 gttcactttg ccatttgcgc ccaggttggc ggagtatttc aggaagagat tcagcatctc
     2341 ctggtgaaag cgacgcgcct ggtgcgggtt atcgaggaaa atgcagttct ctttgacgcc
     2401 tggcgtattg aaatcgttag aggtgctacc cagcgccatg accagggtgt cataggcgat
     2461 tttacgttcc ggaaccagca gttcaccttt ctcgtcgcgt agttctgcaa tagtgattgt
     2521 tttcgcttca cgatcaatat caatgacgga acccaactgg aactggaaac catgattgcg
     2581 cgcatgggcc agatagctca acgcatcgac gccttcatca agcgagccag tcgccacttc
     2641 gtgcagcagc ggtttccaca ggtggctgtg gttacgatcg accagcgtaa ttttggcttt
     2701 tttcttacgt cccagcttat gccccagctg tgttgccatt tccagcccac cagcaccgcc
     2761 gccgacaatc acaatctttt tcaatggcgt agtcaacgtg acccccttaa atttattaac
     2821 caattgttaa ttaatggtta ttaacatagc tcttaattaa caacaggtta cagcaatgaa
     2881 aatgttcttc gctattgaga ataacatgaa tggtgcattg gtcataccaa aattgatgtt
     2941 aatcaagttt tgttgctgaa aagataggcg actttaccgg gtgagatcaa aaaatagagt
     3001 ttattgttta ttatttgagc aaattcggaa gagtaatata gggtgaagcg tatatcgagg
     3061 gcgtttggtc gtcctgataa gatgcgcagc atcgcatcag gcattgatgc tcgactgtcg
     3121 gatgcggcgt ggacgcctta tccgacttac agaattcgga gcgcatcagg taccggtaag
     3181 gttacctgat gcgacgaggg catttacccg agggttttga acgctttaat gcgctgtaaa
     3241 tgcggggaga tattcttgaa tttgtgcgtc tgctcttcgt cccagacaat ctcgtaataa
     3301 tgatgcaact cttcagatgt ccgctggctg ttaagtgctt catcattacg cgacaaaata
     3361 accagacaac gatcgcgatt cttctcgcgg aagttggtca cacacttagt ggcaatatcg
     3421 gcatactctt ccgggcgatc aatcttgcct tccatgttct cataagggaa caaattaggg
     3481 ttgaagatca cctggcggat atcgcaaaga aaaccaatcc gttccgccca gtatccgccc
     3541 aggccaacgc cgcaaattag cggacgctcg tcaacgttca gttgcaacat tttgtccact
     3601 tctttaagca gatgctgcat atcatgtttc ggatgccgcg tactgtagct tatcaagcgt
     3661 acatccgggt caataaactg caattgtaag actttctcgt ggttacccgg actgttagag
     3721 tcaaaaccgt gtaaatagat aatcatcgct tcctcacata agccagggtt agtgacctgc
     3781 tttctcttcc tgccagcgtt cgtgtaactg attcagacgg gtgctgatcg ctttccagcg
     3841 agccgagtcc atcagttcct gtcgcgaaaa tgaaccttta tgatacaaac gtgtaacacg
     3901 ttctgccttg atcggtgaca gattatctaa cacgctgacg gcccctttac gattattgca
     3961 gaccaggatc atatcgcaac ccgcatccag tgatgcctgc ccgcgttcgg cataactgcc
     4021 cataatcgcg gcaccttcca tcgataaatc gtcagagaaa atcacgccgt caaaacccag
     4081 ttcctgacgc aaaacggttt tcagccagta gggagaaccg ctcgccggac gcggatcaac
     4141 atcactgtag atcacatgcg caggcataat ggcgtcgagt ttattttcgc ggattaagga
     4201 actgaagacc gacatatctt tagcgcgaat ctccgcttgt ggacgtggat cgcacggtgt
     4261 ttctttgtgt gagtctgccg ttactgcacc gtgtcctggg aagtgtttcc cggtcgtttt
     4321 cattccggct tcatgcatac catcaataaa ccggctggca attgccaggg ctttttgtgg
     4381 atcggcatga taagaacgct cgccaatcgc cgcgctgata tgcccgacat ccagcactgg
     4441 cgcaaagctg atatcaatat ccatagcgat catttcgctg gccatcagcc aacccgcctc
     4501 ttgcgccagt ttgccgccct cttccattcc tgacagcgca gcgaatgatt gcgccgctgg
     4561 caagcgggta aaaccttcac gaaaacgctg cacgcgtcca ccttcctgat ctaccgccac
     4621 caccagatga ttgcgcgaag ctgcgcggat ctggcgcacc agttcacgta actgggcagg
     4681 atcatgatag ttacgcgtaa agagaatcag ccctcccacc agcggatgcg caagtatttc
     4741 acgctcttcc gcgtccagtt cgtaaccttt gacatccaac attactggac ccacactgct
     4801 ctccttattg ttttattaat agctgccgcc aggtgtcatc ggccagcctg ataaattgtt
     4861 gatcgccggt ttgtcgccag cggtactcaa accaccctgc tttgagcatc agcagccagg
     4921 gaaaccatcg cctgacctga cgccataatt gcgccggata aatcttcgcg cgagtggcat
     4981 agtcattgac caattgccgg tgctgttcag tattttccac ccacaccgcc gccagttcca
     5041 gcgcgatatc accatctccg gcatactccc agtcgatgag ttttaaccct gacgcgctat
     5101 gcactaaatt tccggcgtgg acatccatat gcaatggact taagcgtaaa ggccgtggtt
     5161 cccgcgcttt gcgcagacgt tttaacattc gcagccaacc cactgtccgc cgcgccggat
     5221 cgctttgctg ccagtacagt tccagtaacg gcaacagcgt tattcgccag ccaaaacgtg
     5281 gttgttgatg tagataatac agcaagcctg ccagttcgtt ggtatccggc aaatacgttt
     5341 ttaccgcgcc gggcagatag tcgactacca tccagtcacg gagatataaa tgcggcttcg
     5401 gtgcaatgct tgcgggtagt tgtgataaag cccgatactg gcgcaagaac gcggactgcg
     5461 gcgcatcagg atcgtgcggc tgacgcacaa caaaacgctg gccctgatgt tcaatgagaa
     5521 aactcccgcc actaagccca ctattaaacg tcgtgacggg atgaaactgc gggaaaaagc
     5581 gcgacagcaa ttcgtcgcgc atgatgggat tattgctgcg aaacggcacc tttacctgac
     5641 cagataattt cgcccgtctg caccagcatc agctgcattt gtagggtcgg agcgttaacg
     5701 ttgccagagg cgctggagta cagcacgtaa tgagcgccga cattgcgggc aatgcctatg
     5761 gctttactac gggtacctaa actgtcctgc ggcgacaaac ctaactgttg cttagccatc
     5821 gacagctgct gggcggaaac cagggtaaat ttcccgttat tagccagcgc atttcgcagc
     5881 gtttcggtcg cttctgcggc attcagcgaa ccgttagtac ggttgttaac gctatcaacc
     5941 agcaggacgc tacccgcagt caccccgtca gccccaagca tcttactgac catcggctgc
     6001 attgcgccat tccagtcata atggcgaata tgcggcgcag gcggtgccgt ttgatcttcg
     6061 tgctcaattg ggcctggctg ctgcgggatc gtcggcaccg agggcactgt ggggacaggc
     6121 tgttgtggct cggctggttg ttccggcgct ggtttcactt cttctaccgg tgcaggttca
     6181 cgttgcccca cacacccggc gagaaacatc gccagcgcgg taatcaaggc gtagcgactc
     6241 atttttgtca tcaagattca ccccttacaa atatagataa agtctgactt tgtgcgcccc
     6301 cagaaaattg gcgctgccgt acagcgttac cgccgaatgt gcgggaatgc taacgctgcg
     6361 tggcctttcc agaggatgca tctccagccc tctggcgtca taccagtaaa aacgataatg
     6421 aacggtaacg ggttcttgcc tttcgttata aagcgttgag gatgctgaag gttgaatatc
     6481 agacgtcgaa aggacgggct tttctgcact gatgcccgca gccaataaag atgactccat
     6541 caccagcgat tgctcatcat tcaccggaat ttccggatgt gaacgacagc ccaccagcag
     6601 taacaacgcc agagacacca gcccaaagca tccttttctc atcgttacag acctttatgc
     6661 gccagcattg gtcccagcgg acggccaccc aacaagtgca tatggatgtg gtaaacctct
     6721 tgtccgccat ggcggttggt attcatgatc agacgatagc catcttcggc aataccttct
     6781 tgctcagcaa tttttgccgc tacggtgatc atgcgtccca gcgcctgctc atgctcagct
     6841 gacacgtcgt tcacagtcgg aatgaggata ttcggaatga tcagaatatg cgttggcgct
     6901 tgcggcgaaa tatcgcgaaa cgccgttacc agatcatcct ggtagacgat atcggagggg
     6961 atctcacgac gaataatttt gctgaatata gtttcttctg ccacgacatt ttcctttttc
     7021 aaatagccat gcgttgcgcc atgctacgcc gcgccacact gcgagtatag agtatggtcg
     7081 agttaaacct gctctttcaa ccttaactca cgatttctta agcaaaaaat cgttttgctg
     7141 ccgtatatat cgccattatt cccatttctg cctgcgaatg cgctgaaaag acattctctg
     7201 tttggtttac acgacaaata tgaatgcgta tatttctcat ttgcatttac aaacaaaatt
     7261 attcgcacac taaatataac cgctcatcac cctcctccgg catgagcctg acgacaacat
     7321 aaaccaagag atttcaaatg ctttcaacac aatttaacag ggataatcaa tatcaagcca
     7381 gcaccaaacc atcactactt gccggttgca tagcattggc actattacct tctgccgctt
     7441 ttgccgctcc agccactgaa gaaacggtga ttgttgaggg ttcagccaca gctccagatg
     7501 atggcgaaaa tgattacagc gtaacgtcta cctctgcggg taccaaaatg cagatgactc
     7561 aacgtgatat tcctcagtcg gtcactattg ttagccagca gcggatggaa gatcagcagt
     7621 tacaaacgct gggcgaagtg atggaaaaca cgctggggat cagcaaaagt caggcggatt
     7681 ccgatcgtgc tctttattat tcccgcggat tccagatcga taactatatg gttgatggta
     7741 tccccaccta ttttgaatcg cgctggaatc tgggcgacgc actttctgat atggcactct
     7801 ttgaacgcgt agaagtagtg cgtggcgcga caggactcat gaccgggacg ggtaatccat
     7861 ctgcggcaat taatatggtt cgaaaacacg cgaccagtcg tgaatttaaa ggcgatgtct
     7921 cggcggaata cggtagctgg aacaaagaac ggtatgtggc ggatttacaa agcccactca
     7981 ccgaagacgg taaaatccgc gcgcgaattg tcggcggcta ccagaataac gactcatggc
     8041 tggaccgcta caacagtgaa aagaccttct tctcgggcat tgtcgatgct gatttaggcg
     8101 atcttacgac gctttcagcc ggttacgaat atcagcgcat tgatgttaat agccctacct
     8161 agggcggttt accgcgctgg aatactgatg gcagcagcaa cagttacgat cgtgcacgca
     8221 gtaccgcacc tgactgggcg tacaacgata aagagatcaa caaggtcttt atgaccctga
     8281 agcagcggtt tgctgatacc tggcaagcga cactgaatgc cacccactct gaagtcgaat
     8341 ttgacagcaa aatgatgtat gtcgatgcct atgtaaacaa agcggatggt atgctggttg
     8401 ggccatacag taattatgga cctggctttg attatgtcgg cggcaccggt tggaacagtg
     8461 gcaaacgtaa agttgatgcg ctggatttgt tcgctgacgg tatttatgaa ttgtttggtc
     8521 gtcagcacaa tctaatgttt ggtggcagtt acagcaaaca aaacaatcgt tacttcagtt
     8581 catgggccaa catcttcccg gatgaaattg gcagtttcta caactttaat ggcaatttcc
     8641 cacaaaccga ctggtcacca cagagcctgg cgcaggacga taccacacat atgaaatcgt
     8701 tatatgctgc cactcgagtc acccttgccg atccgctgca tctgatcctc ggcgcacgtt
     8761 ataccaactg gcgggttgat acgctgactt acagcatgga gaaaaaccac accacgcctt
     8821 acgctggtct ggtgtttgac atcaatgaca actggtcgac ctacgccagc tatacctcta
     8881 ttttccagcc gcaaaatgat cgtgacagtt caggcaaata tctggctcca atcaccggta
     8941 acaactacga gctgggtctg aaatcggact ggatgaatag ccgtctgacc accacgttag
     9001 ccatcttccg tattgagcag gataatgtcg ctcagtccac cggtacacct atccccggca
     9061 gcaacggcga aaccgcctat aaagcggtgg atgggacagt cagtaaaggg gtggaatttg
     9121 aactcaacgg cgcaattacc gacaactggc agctgacatt tggcgcaacg cgctatattg
     9181 cagaggataa cgaaggaaac gccgttaatc ctaatctgcc acgcaccacg gttaaaatgt
     9241 tcaccagcta tcggttgcct gtcatgccag agttgacagt cggcggtggt gttaactggc
     9301 aaaatcgcgt gtataccgac accgtgacac cgtatggcac cttccgcgcc gagcaaggta
     9361 gctacgcgct ggtggatctc ttcacccgct accaggtgac gaaaaacttc tcgttacagg
     9421 ggaacgtcaa taacctgttc gacaaaacct acgataccaa cgtggaaggt tctatcgtct
     9481 acggcacacc gcgtaatttc agcattaccg gcacgtatca attctgaata acacctgtaa
     9541 aaaaggcagc catctggctg ccttagtctc cccaacgtct tacggattag tggttacgga
     9601 tgtactcatc catctcggtt ttcaggttat cggatttagt accgaaaatc gcctgaacac
     9661 cagaaccagc aaccactacg cccgctgcgc ccagtttctt caggccagcc tgatccactt
     9721 tagacacatc agcaacgctg acgcgcagac gggtaataca tgcgtcgagg ttagtaatgt
     9781 tttctttacc accaaatgca gcaaccagag ccggtgccat ttcgctggta cctgtcgctt
     9841 ttgcatcttc agtcgcgtct tcacgacccg gcgttttcag atccagtgct ttaatcagca
     9901 cgcggaagat ggtgtagtaa acaatcgcat aaccgatacc gacgatcggg aacagccaca
     9961 gtttgctgct gttaccagac agaacgatga agtcgatcag accgtgcgag aacgacgtac
    10021 cgtcacgcat ccccagaaga atacagattg ggaatgccag gcctgccaga atcgcgtgga
    10081 tgatgtacag gatcggcgca acgaacatga aggagaactc gatcggctcg gtgataccgg
    10141 tcaggaacga ggtcagcgcc gcggagatca taataccgcc cactttcgcg cggttttctg
    10201 gtttagcaga gtgccagata gcaattgcgg cagctggcag accgtacatt ttgaacagga
    10261 agccaccaga cagtttaccc gcagtcgggt cacccgccat ataacgcgga atgtcgccgt
    10321 ggaaaacctg acctgctgcg ttggtgtatt caccaatctg catctggaaa ggtacgttcc
    10381 agatgtggtg cagaccaaac ggtaccaggc aacgttcgat gaaaccgtaa atgccaaacg
    10441 caactaccgg gttctggtaa gcagcccact gagagaaggt ctggattgca gaaccaatcg
    10501 gcggccaaat gaaggacagc acaacgccag taaagatggc agccaggcca gaaatgatcg
    10561 gcacaaagcg tttaccggca aagaagccaa gatactcagg cagcttaata cggtagaaac
    10621 ggttaaacat gtacgctgcg atcgcaccgg agataatccc tccgagtacg ccagtatccg
    10681 ccaggtgttt agaggcgatt tcttcagcag gtaaatgcag taccagtggc gcaaccacgg
    10741 ccatggtttt aaccatgatg ccataggcaa caactgcggc cagcgcggat acgccatcgt
    10801 tattggtaaa gccgagggcg acaccgatcg caaaaatcag tggcatgttt gcaaagacgg
    10861 aaccgcctgc ttctgccata acatgcgata caacggcggg cagccagctg aaattcgcgg
    10921 aaccgacgcc cagcaggata cctgcgatag gcagtacgga taccggcagc atcagcgatt
    10981 taccgacctt ttgcaggtta gcaaatgcat tcttaaacat aattgagagt gctcctgagt
    11041 atgggtgctt tttttacgtt ctcacgcgtg gcaagggggg agagcctcgc cgtgtacagg
    11101 gcatctaagc gccctttatt tattacacag agtaaaataa ttcagtgcca atttgtttga
    11161 cggctatcac gtttcaactt cagatcattg gagaagttgc acattttgac aaaacatatg
    11221 tcacaatatt tcaaacgtgg ttattcaccg cccaattttc ggcggtgaac tttactcgtt
    11281 ttagccatta attacgagct ttaaaaaaac tcattcaacg gatggattga aggcgggaaa
    11341 cgtcgatgtg aaacagacgg gcgaagttat cggtggttac ctgcgccagt tcttcaacgg
    11401 caacaccttt caacacagcc atgtattctg caacgtcacg aaccatcgca ggttgattct
    11461 cttttcctcg atgcggtacc ggcgcaaggt aaggtgagtc agtttccacc agtaatcgat
    11521 ccagggggac ataacgcgca gcatcgcgca gttgctccgc attacggaag gtcacaatgc
    11581 cggaaaagga gatgtaaaat ccgagatcca gtaatttacc cgccgtttct ctgtcctctg
    11641 taaaacagtg tagtacgcca ccgcaatccg tcactttttc ttcgcgcaga attgccagcg
    11701 tatcggcacg ggcgtcacgg gtatggacga taaccggctt gttcagttca cgaccaatct
    11761 ggatatgatg gatgaaggac tcttgctgac gtactttagt ttccggtgtg taataataat
    11821 ccagcccggt ttcacccagc gctacaacac cctcttctgc cgccagacgg cgtaaatctt
    11881 ctacatcgta gggatcattc tggttgagtg gatgcacgcc acaagaaaat acgacgttgt
    11941 cacgttcgcc taccagatcc cgcatatgta aataacccgg taatgttgtg gcgactgcca
    12001 gacaaaattt cacatcgcgt gcggcggctt tcgccagaac gtcatccacg tccttatgca
    12061 aagattcata atccagacca tcgagatggc agtgtgagtc gactaaaaac atgatgtctc
    12121 tcttaaagat gaggaaccgg tagcacaacg cccggttgca ggtaatgctc aatacgcagt
    12181 aaaagatcgg tgatgagaag ctcgcggttg atgcctgtaa cagacattaa ctgttcacga
    12241 atgtggcaaa catcccccag tatagcctgc aggcgcgagg gagaaagatg gtttgccagt
    12301 tcggcgacca ggcccggcac atcaacattg gtcacctgcg cagcaccatg atggcgtttt
    12361 agcgcatcca tcagcaacgt tgccagccag tgtaaacgcg ccggagcttg ttcatgatta
    12421 agggccgcta gcagcgaata ccagtcgccc gatggcacgc tatatgccaa cgcctgacac
    12481 aatgtttcac gagcctgcca gttatctccc tgaaacaacg ccagtgccgc gccaggcgaa
    12541 ccggcgctta agcgcaatgc ggcaagtaat gcatcctgtg acattgtcac ttcgcgtgaa
    12601 agccaggtca cggcgtactg ttccggcggc ggcgcaaggt aatgtaaccg acaacgacta
    12661 cgtaatgttg ccagtaaacg ttcaggctcg cgggtagcca ggaaaaacca ggtttctgct
    12721 ggtggctctt caagcgtttt cagcaatgcg ttagccgcgg cgtcggtcag taaggcagca
    12781 tcggttaccc agacaacttt cgcaccacct aggcgtgcgt gctcattcag cttttcggtg
    12841 acctcacgta ccgcatcaat gcccagcata tttttccctt tttcgggagc cagggtgtag
    12901 taatcgggat gcgtgccagc ctgcatcaac tgacatccac gacagtgacc gcaacttttg
    12961 tggccctgcg gttgttggca gagtaaataa cggctcaggg cgtagattaa agcatcatcg
    13021 cccatgcccg gtaacgcctg aatgagtagc gcatggtgac ctcttccggc ctgatagctg
    13081 gctaccagtt tttcgaaatc aggtcgtaac catggatacc atctcatgcg tccaactcct
    13141 taacccagtg ggtcacggta gtgcggattg catccatcac ggcctccagc ggctgggtgg
    13201 catcaatggt atgaatactt ttatcttgtg cagccagttc cagatagcgg gcgcgggtgc
    13261 gattaaagaa atcgaaagac tcttgctcaa tacgatccag ctcgccgcgc gcacgcgcgc
    13321 gttttaagcc aacttccggg gtaacatcga gatagagcgt taagtcgggg cgaaaatccc
    13381 cgagaacagc atcacgcagt gttgccagca tatgttggtc aataccacgt cctccgccct
    13441 gatacgcctg agtggagaga tcgtggcgat cgccaatcac ccaggtgccg ttagccagag
    13501 ctggtttgat gaccgtttct accagttgaa cgcgcgcggc ataaaacatc agaacttcgg
    13561 ctttatcggt aatgacttca tcgcctaccg atttgatatc cagcaccagg cttcttaact
    13621 tttcggcaag ttgcgtaccg ccaggttccc gagtgaaaac catgtcgcgg atacccagtt
    13681 gctcaagcgt ctcaaccacc acattacgcg cggtagtttt gcctgcgcct tccagcccct
    13741 caatgacgat atacttactg cgcatttttt tccttaagca ctttcagata atcctgcaca
    13801 gacttgttat gactggcaag attggtatta aacgtgtgac cacctttacc atcggccaca
    13861 aaatagagat acggcgtttt tgccggatgc gcagcagcct tcagcgaatc cgcccccggc
    13921 gtagctatcg cacccggcgg caaaccggta atggtatagg tgttatacgc tgtcggcgtt
    13981 tccaggtctg cacgagaaag tttgccatta taacgctctc ccatcccgta aatcacggtt
    14041 gggtcggtct gcaggcgcat accaatgcgt aaacggttga taaataccga ggcaacctga
    14101 tcgcgttcac tggcaacggc ggtttctttt tcgataattg atgccatcgt caccagctga
    14161 tttttatctt tataaggcag accgtccgca cgcccttccc aggcgctatc gaccgctttc
    14221 accattttct tgtgcgcttg cttgagtaac gcgacatcgg tggtattggc ggtatacatc
    14281 caggtgtctg gccagaacca accttcaatc cactccgggt tttccagttc aagtgcctgc
    14341 gctacggtgg cgtacttatc gtcactcagc gtgtgcttga tatacggagc ctcacgcaat
    14401 tgcttgaggt agtcgctcag acgcatccct tctaccagtc gcagcgggaa ctgtgcttct
    14461 ttaccgcttt ccaacaattt cagcatctcg cgcacggtca tctgcggtgt aaagcggtaa
    14521 gtcccggctt taaagtgaga aagatccggt tcgatacgca gcagccattg aaaaacccgt
    14581 gggcgattga tgatcttatc agcataaagc tgttcaccga gcgccagacg tccggtccct
    14641 ggcttcaggg taaatatcgt ctcttctttg ataagcaatt tgctgtcggc aagatggcga
    14701 accttccaga cgcccacacc agcggcgata cccagtacca ccagcaataa caagattatc
    14761 aataacactt ttttcatgac taattcgggc gctcacaaag tggggctaaa tattcatata
    14821 acgttgctga cgaaaaggag acatcgccac aggcacgtac gggcatcact ggcattaacg
    14881 cattacaaat aaccatctca tctgcctgca acgcctcttc cagagaagct tgcacttcga
    14941 caagctgata agaggattgt gccagcaaac ggatacagaa ttgtcgcata atgccgttaa
    15001 cacctgcctg atccagtcgc ggcgtataaa ctacgttgcc cttccgccag aacaaattag
    15061 ccgcacagca ttccgtaacc cacccttcgc tgtcaaggac cagcgcctca tcagcgtttg
    15121 tctgctcaag atgagagcga atcaatactt gctcaagacg attgagatgt ttaatacctg
    15181 caagatgagg attgcgcccc agccgcaccg ggcttagcgc caacgtcatc ccctcgttac
    15241 gcaaacggtc gtaatgtgca ggataagccg taacggagag aatccgcgtt gccggtccac
    15301 tgttcaatgt gctgtaccct cgcccgccac taccgcgact gatcacgact ttcagtacac
    15361 cattttgctg ttctgctgcc agcgttttca tctcttgttc aagctgtggc cagaagtcac
    15421 aggaaatcat caaccgctga caagcatcct gtagtcgctg gatatgcgcc gataacaaac
    15481 tgactttacc gtcgataact ctggcggtgg taaaacaacc atcaccaaac tgcgttgccc
    15541 gatcgcttac tgccagcgat tcctgcttat aaccgttaat taagaacata ccggctcctt
    15601 atcagagaat tgttagtgtg gcagcatgtt cactacggaa caagtcggaa taaaagctaa
    15661 gaaaaaaggc ccgcaagcgg accttttata tgggtggaaa atgacaactt agatcttttt
    15721 aaagatcaaa gaaccattag tgccaccgaa gccgaaggag ttacacagcg tgtattccat
    15781 tccgctgacc tgacgtgctt catgcggtac gaaatccaga tcgcaacctt catccgggtt
    15841 atccaggttg atggttggcg gaacagcctg atcgcgcagc gccaggatgg agtagataga
    15901 ttctactgca cccgccgcac ctaacaggtg accggtcata gatttcgtgg agcttaccaa
    15961 cacacggctt gcagcttcac cgaagatggt tttcaccgcc tgcgcttcag ctttatcgcc
    16021 agccggcgta gaagtaccgt gcgcgttaac gtagccaatc tgactcgctt caatacctgc
    16081 atcacgcaga gcatttgcca ttgccagagc tgcgcctgcg ccattttctg gcggtgacgt
    16141 catatgataa gcatcgctgc tcataccaaa gccgacgagt tcagcgtaaa ttttcgcacc
    16201 gcgttttttc gcgtgttcgt actcttcaag taccagcata ccggcaccat cgcccagtac
    16261 gaagccatca cgctctttat cccacgggcg gctcgccgct tgcgggttat cattgcgggt
    16321 agataatgca cgtgccgcgc caaaaccacc aacacccagc ggcgtactgg ctttctctgc
    16381 gccacctgca accatcacgt cagcatcgcc atacgcgata atacgcgcag catgaccaat
    16441 gttgtgcacg ccggaagtac aggcggtcgc gatagagatg cttgggccac gcaggccata
    16501 catgatagtc agatgacctg ccaccatgtt cacaatcgtt gacggaacga agaatgggct
    16561 gatcttacgt ggaccaccgt tcatcagaga tgtgtggttt tcttcgatca gtccgaggcc
    16621 gccaatcccg gagccaattg cggcaccaat gcgggttgcg ttctcttccg ttatttcaag
    16681 gccagaatcc tgcatggcct gaacgccagc gacaattcca tattgaatga aggcatccat
    16741 cttgcgctgt tctttgcgcg agataatgtc ctcacagtta aaatccttta ctaagccagc
    16801 aaatttcgtt gcataggcgc tagtatcgaa atggtcgatt aggctgatgc cactctgacc
    16861 ggcaagcaga gctttccagg tagactctac ggtattgccg acaggagaca acatgcccag
    16921 tccggtcaca actacacgac gcttagacac gtttgtcctc cagggaggga aaaaatgatt
    16981 ctagtgggac aaaaagataa aactcaggcg gtcgaacgac cgcctggaga tgttcactta
    17041 cgcctggtgg ccgttgatgt aatcaatggc agcctgaacg gtggtgattt tctcagcttc
    17101 ttcgtccgga atctcagtat caaactcttc ttccagagcc attaccagct caacggtgtc
    17161 aagagaatcc gcgcccaggt cttcaacgaa agaagcattg ttggtaactt cttcctgctt
    17221 aacgcccagc tgttcgccga taattttctt aacgcgttct tcgatagtgc tcatactctt
    17281 aaatttccta tcaaaactcg ctttcgcgat ggttttcgta gtgtataaaa tgttgaaaaa
    17341 tttgcaacta aatcccggca ggtcttacca cgattttacg ttattttgag gccttacccc
    17401 ccaataacgc aaatcatttt gcactaatta ttactcataa ccacgcagac tgcgcgcaac
    17461 atgagcattt tgtgcaaatc gcggtcagac catgtacatc ccgccgttca catgcaaagt
    17521 ttcacccgtg atgtaagctg cttcgtcgga tgccaggaat gcaaccgcgt tggcgatttc
    17581 ctgtgcgccg ccgaggcgac ccgcaggaac ctgcgccagg atacccgcac gctggtcatc
    17641 gctcagcgca cgtgtcatgt ccgtttcaat aaagcccgga gcaacaacgt ttacagtaat
    17701 accgcgtgac gcaacttcgc gcgccagtga tttactgaag ccgatcaagc ccgctttcgc
    17761 cgcagcgtag ttggcctgac cgccatttcc catggtacca accacagaac cgatagtgat
    17821 aatacgacca tgacgctttt tcatcatagc gcgcattacc gcttttgaca gacggaaaac
    17881 agatgaaagg ttggtttcga taatatcgtt ccactcttca tctttcattc gcattaacag
    17941 gttatcacga gtgataccgg cattattgac caggatatcc acttcaccaa attctgcgcg
    18001 aattttttcc agaacagatt cgatagatgc cgggtcggtc acattcaaca tcagaccttt
    18061 gccgttagca cctaaataat cactgatcgc ctgagcgcca ttttcactgg tcgcagtgcc
    18121 aataactttc gcgccacggg ctgcgagcgt ttcagcaatt gcgcggccaa ttccgcggct
    18181 tgcaccggtt accagtgcga tttttccttc aaaattcatg attttcctct tttaaagctc
    18241 gagcgccgct gccatcgctg aaggttcgtt cagcgccgag gcggtcaggg tgtcgacaat
    18301 gcgtttcgtc aggccagtta gcactttgcc cgggccgact tcatagagat gttctacgcc
    18361 ttgcgctgcc atgtactcaa cagacttcgt ccactgaacc gggttataca actgacgtac
    18421 cagtgcgtca cgaatggcat caccattggt ttcgcatttc acatcaacgt tattcacaac
    18481 aggaactgtt ggtgcgttaa aggtgatttt cgctaattct actgccagtt tgtcggctgc
    18541 tggtttcatc agcgcacagt gagacggtac gctcactggt aacggcagag cgcgttttgc
    18601 gcccgcagct ttacaggcag cgccagcacg ctcaaccgct tctttatgac cggcaataac
    18661 cacctgtccc ggagagttaa agtttaccgg agaaacgacc tgaccttctg cggcttcttc
    18721 acacgctttc gcaatagacg catcatccag accgatgatt gccgccatag cgcccgtgcc
    18781 ttctggtacg gcttcttgca tgaacttgcc gcgcatctca accagacgca ccgcatcagc
    18841 gaaatcaatc acgccagcgc aaaccagcgc ggagtattcc cccaggctgt gaccggccat
    18901 cattgccggt gctttaccgc cctgctgctg ccatacgcga tacagcgcaa cagatgcagt
    18961 caacagcgct ggctgggttt gccaggtttt attcagttct tcagctggcc cctgctgggt
    19021 cagcgcccac aggtcgtagc ccagcgccgc agaagcttca gcaaacgttt cttcgacaat
    19081 tggatagctc gccgccatat cagccagcat tccaacggtt tgagaaccct gtccagggaa
    19141 cacaaatgca aattgcgtca tgttttaatc cttatcctag aaacgaacca gcgcggagcc
    19201 ccaggtgaat ccaccgccaa aggcttcaag cagaaccaac tgccccggct taatgcgccc
    19261 gtcgcgtaca gcttcatcca gcgcgcacgg gacagaggcc gcagaggtat taccgtggcg
    19321 atccagcgtc accacgacat tgtccatcga cataccgagt tttttcgccg ttgcactgat
    19381 aatacgcagg ttagcctgat gcggaaccag ccagtccagt tgagaacggt caagattatt
    19441 cgccgccagc gtctcatcaa cgatgtgcgc cagttccgtt accgcaacct tgaagacttc
    19501 gttgcccgcc attgtcagat gaattgaatt ctctggattc acgcggtcgg cgtttggcag
    19561 cgtcagcaat tcaccataac taccgtcggc atgcagatgg gtggagatga ttcccggctc
    19621 ttcagaggca gccagcaccg cagcgcccgc gccatcgcca aaaataataa tagtcccacg
    19681 atctgttgga tcgcaggtgc gcgccagtac atcggaaccg acgaccagag catacttcac
    19741 cgccccagat ttcacgtatt gatcggctac gcttaatgca taggtgaaac ctgcgcaggc
    19801 tgctgcaacg tcaaatgccg ggcaaccttt aatgcccaac atgctttgaa tctgacaagc
    19861 tgcgctcggg aaagcgtgcg tagcagaagt cgttgccaca acgatcaggc caatctggtc
    19921 tttctcaatg cccgccatct caattgcgcg tgtcgccgct tcaaagccca tggttgaaac
    19981 ggtttcgttt ggcgcggcaa tgtggcgttc gcggataccg gtacgagtga caatccactc
    20041 gtcagaggtg tccaccattt tttccaaatc ggcgtttgtc cgcacttgtt cgggcagata
    20101 gctgccagta ccaataatct tcgtatacat gtacgctcag tcacttttcg gttatatacc
    20161 gtcacttgca aactgcgagt tcgctggcag cgtcctgcta ccgcagagtt ccgcttttgc
    20221 caccgtccag cagctcaaaa ccagctgggt atacagattc caggcgagcg gcaattcgct
    20281 gaggaacttg tcgctgcacc gcctgcactg cctgttcaat cgcgaccgca aaagctcgct
    20341 gattggctgc accatgactt tttatcaccg tgccgcgcaa tcctaacaga caggcgccgt
    20401 tatactggtc ggggttgagg tgactgaatc gcctcgtcag gctcttttgt agccaacgct
    20461 ttaataacag tagccaccac gaccgttttt tcccttcacc ctgagatttc agcagagaaa
    20521 ggaacatcct gacaacacct tccatcgtct ttaacgtgac atttcctgta aagccgtcac
    20581 aaaccagcac atctgtcttg ccagttaaca actcattggc ttcaagatag ccgatataat
    20641 tgatagaagg gattgtttta agcaccgctg aggcatcccg aatactgtcg agacccttta
    20701 cttcttcttc accaatattg agcaacgcca cgcgaggatt gggaatttcc accacctctt
    20761 cagccagaac tgagcccata atggcaaatt gcaccaacat tgtgctgtca caatcgacgt
    20821 tggcccctaa atcgaggacc accgttttgc ccttttgctg atgtggtaat accgtcacca
    20881 gcgccggacg ctcaatcccc tccaggggct tgagtaataa ttttgccagc cccatcagtg
    20941 ccccggtatt accggcactg acacaggctt gcgctcgacc ttctttcacc agctccaggg
    21001 ccatacgcat tgaactccca cgactggcgc ggatagcttg cgaaggccgg gcatcactgg
    21061 cgataactga ctgcgcagga ataatctgca gacgcgaacg ttgttcaaag tcagctttag
    21121 caagtaatgg cgtgatggcg tcgggattgc cgactaaaag aagagtgagt tgcgaattag
    21181 aattcagtgc ctgcaatgct gcaggcactg tcacggaagg gccaaaatcc cctcccatga
    21241 catctaacgc cagggttaga cgtgtcaagg tatcgtcgcc gcccggtttg gtctttcccc
    21301 agttgcctgg ggaaaatcct cactaagctt catcacgcag acgcgtgatt acttagcgat
    21361 gaccttgcgg ccgcggtagt aaccgtcggc agtgatgtgg tgacgcaggt gtttttcacc
    21421 agaagttttg tctacagaca ggctggtgac tgcggtcagc gcgtcatggg aacgacgcat
    21481 gccacgtttg gaacgggttg gtttattctg ttgtacggcc atggacctta ctcctcaatt
    21541 acggtttatc cccgatccaa cggggagcac caattactta cgctttaagc tggctaatac
    21601 ggcaaatggg tttggctttt gcgcttcttc aggcagttca ccaaagacca tgtccgcttc
    21661 ggacacttca cagtgttcag aatcatgcac cggaactacc ggcaaggcga ggatgatttc
    21721 atcttcaacc attgcaagca gatcgatttc accgaattcg ttaacctcaa tcggttcata
    21781 cgcttccggc agtgcttcag cctgttcgtc tgaacgcaca ggactaaaac aatacgttgt
    21841 gtagacctga tgagtaaacg gcttcccgca acgctgacac tcgagcgtta ccgtcacctt
    21901 cgcatcgccg tttaacactg cgagacgttg gttatcgata gcgaacgaca tggagcattc
    21961 cacatcactg tccacactga ctacggattc ggcgacgcgc tcaacctgat caggggtata
    22021 gataccctgg taatcaaggc gtttttgagc cgtacgaacc ggatcgagag tcaggggtaa
    22081 ttttaccttt tgcatagggc gcgcatatta actttgtaac gtcatagagt caaagaaaaa
    22141 ggcagcctgt ggttgccttt tgccaataat tcgcacacat tgcgggttac tgctttattt
    22201 tcgttcagtg ctgataacga acatcaacct gcaaaacgaa gcgcatagtt taaaatggct
    22261 atcatcaata cgctatatct gatggaaaaa aatatgccta aacttatttt agcctccact
    22321 tcgccctggc gtcgcgctct tctggaaaaa ctgcaaatct cttttgaatg tgcagcacca
    22381 gaggtcgacg aaaccccacg cagcgacgaa tcaccgcgac agttggtgct tcgactggca
    22441 caagaaaaag cacaatctct ggcgtcacgt tatccggatc atttaattat tggttcagac
    22501 caggtatgtg ttcttgatgg cgaaatcacc ggaaaaccgt taacggaaga aaatgcccgt
    22561 ctgcaattac gcaaagccag cggcaatatc gtcaccttct atactggact ggcgttgttt
    22621 aattcggcga atgggcatct gcaaacagaa gtggagcctt ttgacgtcca tttccgtcac
    22681 ctgagcgagg cggagattga taattacgtg cgtaaggagc atcccctgca ctgcgcgggt
    22741 agctttaaga gtgaaggatt tggcattacg ctgtttgagc gtttagaggg gcgcgaccct
    22801 aacacactgg ttggtttacc gctaattgcg ctgtgtcaga tgttacgtcg ggaagggaaa
    22861 aatccgctga tgggatagtt ttttgcgccg gatgcggtta cgtcgcatcc ggcaatcggt
    22921 gcaggcggcc tgatgcggcg taaacgcctt atcaggccaa ccgcccatta tcaggcttat
    22981 atgcttgtat tagcgcgcgt tacgcagctt ttgcaaacaa cgcttcaaac cttcatccat
    23041 cggcgcttcg atgcgcatca cctcaccggt ccccggatgg gtaaacttca acgctgcggc
    23101 gtgcaggaac agacgattta atcccgtgcc tgcttcagtg agctgtctgt caaattcacg
    23161 gtcaccgtag cgatcgtcaa aggcaatcgg atgacccgca tactgtgtat gcacacggat
    23221 ctgatgagta cgtccggtta ccgggctaca acgcaccagg gtggcaaatg catagcgttc
    23281 ttccacttta aagcgtgttt ccgacggttt gccttcctga ctgacacgca cgatacgttc
    23341 gccgctttgc agaatatttt tcaataacgg cgcttgaacg ctcttcacat gcgactgcca
    23401 ctgaccgcgc accagcgcca ggtaatcttt ttgcattcct ttttcacgta attgctcatg
    23461 cagagaacgc aacgccgagc gttttttcgc taccagcaac acacctgaag tgtcccggtc
    23521 gagacgatga accagttcaa ggaaccgcgc ttccgggcgc aacgcccgca aaccttcgat
    23581 aacgccgaag cttaaaccac tgccgccatg taccgccgta ccggaaggtt tattcagcac
    23641 caggatatga tcatcttcat ataagatgac gtccgccagc gccgccacct tttgcagatg
    23701 tggcgaaacc gcctcttctt cccgctcagc aacgcgaacc ggtgggatac gcacctcatc
    23761 acccgcttcg agtttatatt caggcttaat acgttttttg ttcacccgca cttcaccttt
    23821 acgcaaaata cggtaaatca tactttttgg tacgcctttc aattgggtac gcaaaaagtt
    23881 atcgatacgt tgccccgctt cgtcagcggt gatagcaaca atttttacgg atggagtctc
    23941 tgttttcatg gtgggcgatt ctaattagcc aacaggattc gcgccactca tttttctatg
    24001 cttatattta gttttgcacc ttattacttc actacgtgat cactttattg atggttatta
    24061 aaccaatcac cagcaagaag tgaaaaaact gtgagtaagc gggtgataaa tggtaaaagt
    24121 catcttgcta taacaaggct tgcagtggaa taatgaggcc gtttccgtgt ccatccttgt
    24181 taaaacaaga aattttacgg aataacccat tttgcccgac cgatcatcca cgcagcaatg
    24241 gcgtaagacg tattgatctt tcaggcagtt agcgggctgc gggttgcagt ccttaccggt
    24301 agatggaaat atttctggag agtaataccc agtctgtttc tttgataatt gcgctgtttt
    24361 tccgcatgaa aaacgggcaa ccgacactct gcgcctcttt gagctgacga taaccgtgag
    24421 gttggcgacg cgactagaca cgaggccatc ggttcacacc cggaaaggcg ttactttgcc
    24481 cgcagcttag tcgtcaatgt aagaataatg agtaagttac gatgaaaaga atgttaatca
    24541 acgcaactca gcaggaagag ttgcgcgttg cccttgtaga tgggcagcgt ctgtatgacc
    24601 tggatatcga aagtccaggg cacgagcaga aaaaggcaaa catctacaaa ggtaaaatca
    24661 cccgcattga accgagtctg gaagctgctt ttgttgatta cggcgctgaa cgtcacggtt
    24721 tcctcccact aaaagaaatt gcccgcgaat atttccctgc taactacagt gctcatggtc
    24781 gtcccaacat taaagatgtg ttgcgtgaag gtcaggaagt cattgttcag atcgataaag
    24841 aagagcgcgg caacaaaggc gcggcattaa ccacctttat cagtctggcg ggtagctatc
    24901 tggttctgat gccgaacaac ccgcgcgcgg gtggcatttc tcgccgtatc gaaggcgacg
    24961 accgtaccga attaaaagaa gcactggcga gccttgaact gccggaaggc atggggctta
    25021 tcgtgcgcac cgctggcgtc ggcaaatctg ctgaggcgct gcaatgggat ttaagcttcc
    25081 gtctgaaaca ctgggaagcc atcaaaaaag ccgctgaaag ccgcccggct ccgttcctga
    25141 ttcatcagga gagcaacgta atcgttcgcg cattccgcga ttacttacgt caggacatcg
    25201 gcgaaatcct tatcgataac ccgaaagtgc tcgaactggc acgtcagcat atcgctgcat
    25261 taggtcgccc ggatttcagc agcaaaatca aactgtacac cggcgagatc ccgctgttca
    25321 gccactacca gatcgagtcg cagatcgagt ccgccttcca gcgtgaagtc cgtctgccgt
    25381 ccggtggttc cattgttatc gacagcaccg aagcgttaac cgccatcgac atcaactccg
    25441 cacgcgcgac ccgcggcggc gatatcgaag aaactgcgtt taacaccaac ctcgaagctg
    25501 ccgatgagat tgctcgtcag ctacgcctgc gtgacctcgg cggcctgatt gttatcgact
    25561 tcatcgacat gacgcctgta cgccaccagc gtgcggtaga aaaccgtctg cgtgaagcgg
    25621 tgcgtcagga ccgtgcgcgt attcaaatca gccatatttc tcgctttggc ctgctggaaa
    25681 tgtctcgtca gcgcctgagc ccatcactgg gtgaatccag tcatcacgtt tgtccgcgtt
    25741 gttctggtac tggcaccgtg cgtgacaacg aatcgctgtc gctctctatt ctgcgtctga
    25801 tcgaagaaga agcgctgaaa gagaacaccc aggaagttca cgccattgtt cctgtgccaa
    25861 tcgcttctta cctgctgaat gaaaaacgtt ctgcggtaaa tgccattgaa actcgtcagg
    25921 acggtgtgcg ctgtgtaatt gtgccaaacg atcagatgga aaccccgcac taccacgtgc
    25981 tgcgcgtgcg taaaggggaa gaaaccccaa ccttaagcta catgctgccg aagctgcatg
    26041 aagaagcgat ggcgctgccg tctgaagaag agttcgctga acgtaagcgt ccggaacaac
    26101 ctgcgctggc aacctttgcc atgccggatg tgccgccagc gccaacgcca gctgaacctg
    26161 ccgcgcctgt tgtagcccca gcaccgaaag ctgcaccggc aacaccagca actcctgcac
    26221 aacctgggct gttgagccgc ttcttcggcg cactgaaagc gctgttcagc ggtagtgaag
    26281 aaaccaaacc gaccgagcaa ccagcaccga aagcagaagc gaaaccggaa cgtcaacagg
    26341 atcgtcgcaa gcctcgtcag aacaaccgcc gtgaccgtaa tgagcgccgc gacacccgta
    26401 gtgaacgtac tgaaggcagc gataatcgcg aagaaaaccg tcgtaatcgt cgccaggcac
    26461 agcagcagac tgccgagacg cgtgagagcc gtcagcaagc tgaggtaacg gaaaaagcac
    26521 gtaccaccga cgagcagcaa gcgccgcgtc gtgaacgtag ccgccgccgt aacgatgata
    26581 aacgtcaggc gcaacaagaa gcgaaggcgc tgaatgttga agagcaatct gttcaggaaa
    26641 ccgaacagga agaacgtgta cgtccggttc agccgcgtcg taaacagcgt cagctcaatc
    26701 agaaagtgcg ttacgagcaa agcgtagccg aagaagcggt agtcgcaccg gtggttgaag
    26761 aaactgccgc tgccgaacca attgttcagg aagctccagc tccacgcaca gaactggtga
    26821 aagtcccgct gccagtcgta gcgcaaactg caccagaaca gcaagaagag aacaatgctg
    26881 ataaccgtga caacggtggc atgccgcgtc gttctcgccg ctcgcctcgt cacctgcgcg
    26941 tgagtggtca gcgtcgtcgt cgctatcgtg acgagcgtta tccaacccag tcgccaatgc
    27001 cgttgaccgt agcgtgcgcg tctccggaac tggcctctgg caaagtctgg atccgctatc
    27061 caattgtacg tccgcaagat gtacaagttg aagagcagcg cgaacaggaa gaagtacaag
    27121 tgcagccgat ggtgactgag gtccctgtcg ccgccgctgt cgaacctgtt gttagcgcgc
    27181 cagttgttga agaaatggct gaagtcgtag aagcccccgt accggttgcc gaaccgcaac
    27241 cggaagtggt tgaaacgacg catcctgaag tgattgccgc cgcggtaact gaacagccgc
    27301 aggtgattac cgagtctgat gttgccgtag cccaggaagt cgcagaacac gcagaaccgg
    27361 tggttgaacc gcaggaagag acggcagata ttgaagaagt tgccgaaact gctgaggttg
    27421 tggttgctga acctgaagtt gttgctcaac ctgccgcgcc agtcgtcgct gaagtcgcag
    27481 cagaagttga aacggtagcc gcggttgaac cagagatcac cgttgagcat aaccacgcta
    27541 ccgcgccaat gacgcgcgct ccggcaccgg aatatgttcc ggaggcaccg cgtcacagtg
    27601 actggcagcg ccctactttt gccttcgaag gtaaaggtgc cgcaggtggt catacggcaa
    27661 cgcatcatgc ctctgccgct cctgcgcgtc cgcaacctgt tgagtaataa ttagctcaaa
    27721 gtaatcaagc cctggtaact gccagggctt ttttatttca tctttgaatc aacatattaa
    27781 aattcatcaa atggcatcct tgctaaccaa caatgcaaaa taggcaaaaa aaatgcccgc
    27841 cccggcgatc ccagagcagg cagacaaaac atacccagtt tcatgatatg ttttaaagcg
    27901 agcttatttg ttgagctgga agagcgacaa tccctgcata tcggtaaatg ctttatacga
    27961 tgcctgcaat gccgtttgct gcatgatgta agatgaaata gttgcattcc agtccacatc
    28021 aaccagatcg ctcatctgct gcgtttgccc taaagcgcga tcgctaccta aggaatccag
    28081 cgactccagt tcgttcagct gcgtgcctaa ttccgcgcga acagtcagca cattgttcag
    28141 cgagtttttc agtccgcggt tggttttatc taacgccgca gcggcggttt ctttatccgc
    28201 ttcgctatcc gcgaccggcg ttttcagggc tgcgatggca ctatccagca tggcaaaaag
    28261 attggtttca gaagcgctac cgtctggttc cgctaccgcg ttgctggtaa tactgtcgaa
    28321 aattttgtca cccgtgtgcc ctatcaccat cgaacgcgaa gcatcgacct gttgtttaat
    28381 actttctgct ccaccgacgt atttcccttt ctcttcgcta aacggcgcag tctctgtttt
    28441 ataaccggca aaaatgtagc gcccgttacc gtcagtggtg ttcgccagat tcagcaactg
    28501 gtcacgaagc ccctgaatat ccgtagccag cgaggcccgg tcatcgtcac tcaaggtgcc
    28561 attgctggcg tagacaattt tttcctgagc attctggata gcagtggtga cctggctaag
    28621 tacactctct tccagtgaca ctttttgagt ggcgaaagta cgtgccagcg tgtactggct
    28681 gttttgcgcc tgtgcctggg agagaacaac agcttgtgat gcagcaatgg gatcgtctga
    28741 agggttaacg actcgcttac ccgtcgacat ctgctcgccg tacttcatcc attctgcctg
    28801 agaattggtg atgccgcgca tgttttgctg gtacatcatc tgggtactga aacgcatttt
    28861 atctcccctt agcgaatgtt aatcagcgca tcaaaaatcg cgtttgccgt ctgcagaacc
    28921 tgcgcattcg ccaggtaata ctgctgaaaa cgttgcagat ttccgtactc ctcatcgaga
    28981 ttgacaccgg aaatcgactg ctgctgattg gaaagctgcg tcaccacatt accttgcgtg
    29041 gtgctactgg ttttcaacgt cgcggtttta ttaccgatat cactcactaa cgaggcataa
    29101 gcgtcgttaa aggatttcgc accacccacc gttttactgt tgctttgcag atccagcagg
    29161 gcctgaccgt tgcggttgtc gctatcaccc gcatcttctt cgctcgccat cgcgattttc
    29221 gcttcgtcgg tgattaatac atccatgttg acgatggcgt cacttactgg tttcagcgtg
    29281 aagctgtcgt taacggcagg cgttcctgta aacgtcaact ccagaccatc aaatgccact
    29341 ttaccgttgg catctggtgt cgccgtaaaa gtggtattgc tggcaaggcg ggtgacctgc
    29401 cactgattat tatcgaacga gattttgtaa tctgtcgcca gtaccacgga ggcatcagtt
    29461 accgtggcac cgatcgcaac gtcacctttg tttttcgtgt tttgcagaac cgcgggctta
    29521 ccgatagtaa agaaatcttc accagcatcg ccgttagcat caaatccggc tttgtgttgg
    29581 ctgttgaaag cctcggcaaa tgccagcgcc agttgtccaa gcgtattacg cgtctggtcc
    29641 agatcctgag aacggaatgt cagaatgccg cccagcgacc cggtattcag taatttctcc
    29701 gggatctcaa tattgcctgc cgtcccatca acgtaagcga cagtcgtacg agaagggtcg
    29761 gcgctggaag gaactgccgc cagttgccgc gccgtacttc cctgaaccag tgagtaacca
    29821 ttggccatcg tgatgttata agtgccgcca tcctgaacgc tgacttctac accaacaatc
    29881 tggtttaatt cgctcaccag ttgatcgcgt tgatccagca gattgttagg tgacgcccct
    29941 gcccccacgc ctgtcaggcg ggagatttga tcgttcaggc tggcgatttg tttagcgtaa
    30001 ttgttgatct gatcaacgct ggcacctatc gcgatattga cctgtttgtc ctggtcgcgc
    30061 agatattgat cggtggtttt aaactgattc accaaccctt ctgatttccc aatcagcgcc
    30121 tggcgcgctg ccgggtcttc cgcgttactc accagcgttt gcaggctggt gaagaaatcc
    30181 tgcatctgtg ttgccagcga agaggtactg gtggagagca tattgtcgat tttcgacatc
    30241 tgctcatagc gggcagtcag gccgctactt tgcgtctgcg ccgcacgtaa ctggttggta
    30301 ataaaagcat catactcacg ctgcacacca gaaacgtaga caccattgcc aacccagccg
    30361 ccagcgccca acgtgctatt ggcctgcgcc ataatagtgg tttggcgggt atatccggca
    30421 acgttatagc tggagatatt attacttgcc gtatttaacg ccgcctgggc cgcgttcagt
    30481 ccgctcatgg cgttattaat caagctggac atggaggttc cttataagcc ttcaattaca
    30541 gagtattatc ggcagcgacc cgccggactt gagttattca gaacagatta tcaatgttca
    30601 tgctgtaggt tttgctcacc ttgtcgctta tcgatttcat ctgctgaatc atgttggtga
    30661 gtttgcgggc atagtgagga tcggtggcat agcccgcgtc ctgtagggcc tgcgccccct
    30721 gttccgcact cgcggcggtc gtcacggcgg cgtagcgcgg gttacgcgtt aacagcccaa
    30781 cgtaatccga caaggcttcc agatacgagc tgtagacgcg aaacttcgct tttactttct
    30841 tcgcttcgcc gttttcatat tcagtcgtgg tgatttcagt aactggccct ttccagttgc
    30901 cagaggcttt gacaccaaac aggttatagc tcggctcgcc gttttcgcgg cggatttgcc
    30961 gttgccccca accagattcc agtgccgcct gagcgaggat caaatgatgt ggcacaccgc
    31021 tttgctggct tgccagttgg gcgggcagcg agagttgcgc gaggaatgct ttactgtcac
    31081 ccggcagcga atcatcgtag ttacgtggca ctgccttttg caccagctgc gaaagcgcct
    31141 gattttgata acgcaccacg gtttcgagcg ggaatttcat cggtactgct ggcgtggact
    31201 cctctggcaa tggttgttct ggcgtcatct gtttaaccat catctctgca agccccagac
    31261 ctttgcccgc cgtcatctgt tgggcaattt gctggtcata catactggta tacagacgag
    31321 tgtgctcgct gctgaacagg ccatcttttg gtaaagcgtc gcgcatgctt ttcaacatca
    31381 tctgcacgaa catcccttcc acctggcggg ccaccggacg gatatttgcc gccggatctt
    31441 cgcccgcttt cgcctttagt tcgttgagtg attgcgcatc ccaggccgca cttgccagta
    31501 gtttgctgtc gctgatcatc agatgatttc cagttttgcc cgcagacatc ccgcactttg
    31561 cattgattgc aaaatagaca tcagatccat cggcgtagcg cccagcgcat tgagcgcgcg
    31621 caccacgtta ttgaggctgg cgctggaacg tacgctttgc agcgaaccgc cgctctggcg
    31681 taaatcgatc tgcgtttgtg gagtaaccac agtctgtcca ccaccaaacg gtgtatctgg
    31741 ctggctgaca ttggcctgac gattgactgt tactgagaga tttccctgcg ctaccgcgca
    31801 gctgtcgagg gtcacttcgc gattcatcac caccgaaccg gtgcgcgagt taatcactac
    31861 tttagcgtcc tgcggggtga cattaacctg catattctgg atatctgcaa ggaaacggac
    31921 ctgggaactg ttgccacttg gtacgcgcac ctgaatagtc cgcgcatcta acgcggtggc
    31981 gctgccatat ccacgcacgc gattgatggt gtcagcgatt tgctgcgcca tgctgaaatc
    32041 ttcgtcgtta agttgcaaat taagggtatt cccgacgcca aactggctgg gcaattcacg
    32101 ttcaataacc gcaccattgg tgatccgtcc accgttcagt tggttcacct gaacactgct
    32161 accgccagcg gaggctcctg cgccgccaac cagaatattg ccctgcgcca gcgcatacac
    32221 ctgactgtca acgcccttaa gcggcgtcat caacaacgta cctccacgca agcttttggc
    32281 atttcccatg gaagaaacca ccacatcgat ggtttgcccc tgacgtccaa acggaggaag
    32341 tgacgctgtc accattaccg cagcgacgtt ttttagctgc atattggtgc ccgtcggaac
    32401 ggtaattccc agctgtgaga gcatgttatt aagcgtttgt gtggtaaacg gcgtctgggt
    32461 tgtctggtca ccggtgccat ccagccccac caccagacca tagccaatca gtgagttttg
    32521 ccttaccccc tgaacactgg tgagatcgcg aatacgctca gcctgagccg ccgtcgtaac
    32581 cagtagaaga attaatgcag agagaaattt aatcaccaca gcctcactta cattggcgac
    32641 aggttaagga agaaacgctg caaccagccc atattttgcg cttcgttaat gtagccattg
    32701 cctacgtatt caatgcgcgc atccgccacc tgagtagacg gtacggtatt gctgccgctg
    32761 atagtgcgtg gattaaccac gccagagaag cgaataaatt cggtaccctg attaatggca
    32821 atctgttttt cacccaccac atgcaggttg ccgttgacca gtacctggtc aaccgtcacc
    32881 gtcaacgtgc cgctaaaggt attgctggca ttggccccgc cctttccgtt gaacgtgtta
    32941 ccaccggagg cttcgacatc ggcacgagcg ttaccaaaca gcccctgcaa atagcgcggc
    33001 acagtatcaa agccaaaatt agttttaccg tcacggctgg cattcgcaga ggagcttttg
    33061 ctggcgctga cgttctcctg caacacgatg gtcagcgtat cgccaatatt gcgtggtcga
    33121 cgatcttcaa acagcggttg atagccatag ttaatcggct gagcagactg gaaaatagaa
    33181 ccgttggcga cgggcgtcgg cccgggaacc ggttgtgcac tggtcgcccc ctgcaccagc
    33241 ggcgtggagg gtatccaggc gcagccggtt agtgaaagca ccaacaagct ggaaatggca
    33301 taagtatgcg cagcgttttt ttgcatggct atcatcttca aaaatcagta aaccggtgaa
    33361 cctgccaccg gttaagcctt agagttgcgt cagtttttgc agcatctgat cggtggtgga
    33421 caccgcttta ctgttgattt cgtaagcgcg ttgcacctga atcatattga ccagttcttc
    33481 cgccacgttg acgttagacg tttcaacata cccttgatac agcagtcccg cgccgttcag
    33541 gcccggcgtg ctttcgttcg gtgcaccaga ggattgcgtt tcggtgtaga ggttttcgcc
    33601 aatgctctcc agcccggtgt cattcataaa ggtggtgaga ttgagctgcc caacctgaac
    33661 cggagctgcc tggccttgtt gggttacgct gaccacgcca tcacgaccga tggtgatact
    33721 taacgcattc gccggaatgg tgatcgctgg ctgcacctga aaaccaccag ccgtcaccag
    33781 ctgcccgttc tgatccacct ggaaagagcc gtcacgggta taggctgatg aaccatctgg
    33841 caacatcacc tggaaaaagc cctgcccttt aatcgcgaca tctttgctgt tgttggtctg
    33901 cgacaggttt ccctggctgt gtaagcgttc agtggcgacc gggcgtaccc ccgtgccgat
    33961 ttgtaatccg gagggtaagg tggtttgttc ggaagactgt gcccccggct ggcgaatggt
    34021 ttgataaagc agatcttcaa acaccgcgcg ctgacgctta aaaccgttag tactgacgtt
    34081 tgccaggttg ttggcaatga cgtccatatt ggtttgctgg gcgtcaaggc ccgttttggc
    34141 gatccataat gaactgatca taaaatgtcc tgtagtggat tagtgacaag cagagcagaa
    34201 gcacgcgaca gtgtgaattt cccctcaccc taaccctctc cccaaagggg cgagggaata
    34261 gtccgcgcgc ttttattgca atgtcatcaa tcgttcaatt aactcatcga cagcagttgg
    34321 ttggcacggc ctgcgttatc atcgacgctg ctgatcacct tcatctgcat ttcaaaacgc
    34381 cgcgcgctgg caatcatgtc gctcattgcc gcaacggcat tgacgttact gccttccaga
    34441 acccccgaca tcacacgcaa ggttggatct gcctgcagta tcggcccacg cgtggcctgg
    34501 ctttctgcgc ttaaacgaaa aatgccgtcg tcaccgcgtt gcacttcgct gcccgtggct
    34561 ttcaccagtt tcagacgccc cactggcgca accgtatttg ccggatcgcc cggattgagc
    34621 gccgagattg tgccatcggc agcaatagtg atttccgccc cttccggcac agcaattggc
    34681 ccagcctcgc ctatcaccgg atgcccctga attgtcagtt gcccggtggg atcaacctga
    34741 atgctgccat tacgcgtata cccttcgctg ccgtcagcgg actgcacggc cagccagcca
    34801 tcctgctgca acgcaacgtc cagcgggcgc gaggtgtaat ccatcttgcc gggcgtcatg
    34861 tctgcacccg gtgttgacgc cgttaccagc gtgcgcgtgg gcagtgaaag cccttccacc
    34921 ggcactgcac gcaaggcgtt taactgcgcg cgaaaaccgg gcgtagaggc attggccaga
    34981 ttactggcgg ttaccgcctg ttgattcagc gtctggctgg ctgctcccat cgcggtataa
    35041 attgcgtgat ccattgagct atcccgtcag cgattagcgt aagttaacca gcgtgttgag
    35101 gatctggtcc tgggttttga tggtctgggc gttagactga tagttacgct gggcaacgat
    35161 catattgacc agttctttac tgagatcgac gttggacgct tccaacgcac cgttggtcag
    35221 ggtgccaaag ttgcccgtcc cggctgtccc caacagcgcc acgctagaag attgcgtcgc
    35281 agaccagacg ttgtcgcctt cggatgccag accttcgttg ttggcaaagt tcgccagtac
    35341 aatctgcccc agcagttgtg tttgttcgtt ggaatagttg ccgacaaccg taccgtcatc
    35401 attgatttga taactcacca gatcgcccgg tttgtagcca ttctgggtgg ttgccacaat
    35461 attgttagcg ccggtatttt gctgcatgga gttgaggaag ctcaggctaa acgtggcggg
    35521 atctgcgccg ttaattgcgc cagtggtaat attctctgtt ggatttgagg tcagaacgcc
    35581 attggcatta aacaccagct tcattgcagg ctcggctgta cctgttggat cactgctatc
    35641 ctgggtgtag acctgccagt tattatcccc ggtcttcaca aagtagacgc tcatgtcatg
    35701 agcattaccc tgactgtcga aaacagtcac cgaacctttt ttgttatagc tatccgcatt
    35761 gctggcgctg aatggcgtaa cagtaggaag cggatcactg gaattcaggt tgatctgcat
    35821 cgatgccgtg gtggtagttt tcgctgccat cagggtattc gggatcgaaa tattggtcgg
    35881 attcgcccct tgctgaatag tcggcggcgt accggttgcc gggtaacccg tcagctgtaa
    35941 accttgcata ttcaccaggt tacggttttc atccagctta aattgtccgt tacggctgta
    36001 gaacaccgaa ccgttgctgt ctaccagacg gaaaaaaccg ttctggctga tagcaacgtc
    36061 cagacctcgc ccggtgttgg tggtcgtgcc atcggtaaag tcctgagtga taccggcaac
    36121 ttttaccccc agtcccactt tcgaaccggc aaacatatcg gcaaaagagg ccgtgcctga
    36181 tttaaagccg taggtggcgg agttggcgat attgttgcca ataacatcga ggttggtggc
    36241 ggcagcgttt aatccgctaa ccgcttgaga aaaggccatg actgactcct gaaatgtgaa
    36301 ggcttaaatt atctgccgta cttcgtcgag ggtggtggtg ccgtaagtgc cgagatccag
    36361 cgtattaccg cggttgccgc ggatcacacc ctgcaccaga gcaaactgca gcggctgggc
    36421 aaccagttgt gtaccaccgt tactcgcgct aatcgctaca ttgtaagaac cgttcggcgc
    36481 agtgctgccg tcagtcaacg taccgtccca ggtgaaactg tgaactccgg cggtcagttc
    36541 accaatatca atggtgcgca caaccgcacc atttttatcg gtgatggtgg ccgtaacttt
    36601 gtctgccgcc tgttgcagct caacaccaaa cggcgtggtc gtggtcacag ctccttcttc
    36661 actgccggtt cccgcaagaa cagtggtgcc ggggatcatc acgccgtgac cgatcaggtt
    36721 actggcctgt aacgactggc tgttatcaat ctgtccggaa atagatccga gtgtggtatt
    36781 gagtttttca atcccgctga ccgtgctgat ttgtgccaat tgcgacgtca gctcgttgtt
    36841 ttccattgga ttggtcgggt cctggttttt cagctgcgcc accagcaaag tcagaaaact
    36901 gctttgtaaa tctgcggcgt tgctgcccgt gagcgaacta ctgctggtgg tactgacgcc
    36961 ggtatttgtc ggatcggtgg tggttaccgc aatggacata gctttctcct ttattgaccg
    37021 agcgtaaggg ttttcagcat catacttttc accgtgttga gcacttcaac attggcctga
    37081 tagctgcgtg acgccgacat ggtgttaacc atctccccga cgacatcaac gttcggcatt
    37141 tttacgtagc cctttgcatc cgccagcgga ttacccggtt cataaaccag tttgtccggg
    37201 gcctgacttt ctataacatc ggcaaccttt acgccgcctg tcgcagcacc tggtgcagcg
    37261 ttaacctgga ataccacctg ttttgcccga tatggctgtc catcgggacc ggtcacgcta
    37321 tcagcatttg ccagattact ggccgccacg ttcaggcgct gggactgggc agttaacgcc
    37381 gacccggcga tatcaaaaat attcagcagt gccatccgtt aatttccgct ctgtaaaacg
    37441 ttcatcatgc ctttgatttg cccgctcaac gcgctaaggc tcatctggta ttgcaggctg
    37501 ttatcggcaa actgggtgcg ttcgcgatcc atatcgacgg tattaccgtc aagcgaaggc
    37561 tggtccggaa tacggtattg cagttctgcg gtaggaggcg tcagcgcctg cgccgggatg
    37621 tgttgcgttg aggtcatcgt cagtgcaacc acactggttg catcccgtcc acgttgcatg
    37681 acttttttaa gttcactggc aaaatcgata tcgcgcgcct gataaccagg ggtatcggca
    37741 ttggcgatgt ttgctgccag cacttcctga cgctgggcgc gcagattgag cgcctcttgt
    37801 tgaaaacgta aggcggcgtc gagcttatcg agcatatctc ctccgcaggt atcaaaattc
    37861 tgccatcagc ttaaatgcct ttacgcgcgc gttatcggcg gaataaacgc aaaatgggtc
    37921 gctatttatg ccgttgatgg tcattgcgga caggtacaat tcacgttgta gaaatggctg
    37981 ggggcgaaaa tgctgacaat aaaacgtagc gtggcgatca tcgcgatact gttcagtccg
    38041 ttaagtacgg cgagcaatct cacatcgcaa ttgcacaact tttttagcgc ccaactcgcg
    38101 ggggtaagtg atgaggttcg tgtttctatt cgtacagcgc ccaatctact accgccatgc
    38161 gagcagccat tgctttcgat gagcaataat tcccgcctgt ggggcaatgt gaatgtgttg
    38221 gcacgctgcg gtaacgacaa acgatattta caggttaatg tgcaggccac aggaaattat
    38281 gtggttgccg cgatgcccat tgtgcgggga ggaaagctgg aagctggcaa tgtcaaacta
    38341 aaacgcggac ggctggatac cctgccaccg cgtacggtgc tggatatcaa tcaacttgtt
    38401 gatgccatta gcctgcgcga tctatcaccc gatcaaccta tccagttaac ccagtttcgc
    38461 caggcatggc gggtaaaagc gggacaacgc gtcaatgtga tcgccagcgg tgatgggttt
    38521 agcgccaacg cagaaggtca ggcgctgaac aatgcagccg tcgcacagaa tgcgcgggtg
    38581 cgcatggtat cgggacaggt agtcagcggc gttgttgatg cagatgggaa tattcttata
    38641 aacctgtaag ctgttaaaga ttacccgtcc cttgccgata aataagcaac acatgataaa
    38701 agcgccctca atgaggaata aaccatgagt attgatcgca cttcgcctct gaagcctgta
    38761 agcaccgttc aaccgcgcga aaccactgac gcgccggtaa cgaacagccg ggcggcaaaa
    38821 acaaccgcct ccaccagcac cagtgtgacg ttaagcgacg cgcaagcaaa actgatgcaa
    38881 cccggcagca gtgatatcaa tcttgaacgt gtcgaagcgt taaaactggc gattcgtaac
    38941 ggtgaactaa aaatggacac cggcaaaatt gccgatgcgc tgatcaacga agcgcagcaa
    39001 gacttgcaga gtaactgatc gtatgacacg tcttgcagag atcctcgacc agatgtccgc
    39061 tgtgcttaac gatctcaaaa cggtaatgga tcaagagcag caacatctct ctatggggca
    39121 gatcaacggc agccagttgc aatggattac agaacaaaaa agctcactgc tggcgacgct
    39181 ggattacctc gaacagttac gcaagaaaga acccaataca gcaaatagcg ttgatattag
    39241 tcaacgctgg caggaaatta ctgtgaaaac gcagcaacta cgccaaatga atcaacataa
    39301 cggctggtta ctggaaggac agattgagcg caatcaacag gcgctggaaa tgttgaaacc
    39361 gcatcaggaa ccgacgctat atggggcgaa cggtcagacc tcaacaaccc atcgcggcgg
    39421 taaaaagatt tcgatctgaa tattatctcc ggcctgcact gcaggccgga atgcattgtt
    39481 acaccgtccg gcgggcaaat tctttaactt tgaagcccag taccgccagt gcagcgaagt
    39541 acgcggcaat ccccgccagc acgaccgcca ttaaacgcag taagcgccag ggcatggtac
    39601 ccaatgacca ctccggcatg atatgtaaca tacctaaaag cacgccagac atcaccagta
    39661 ccgccaccac cagacgcaac agaaacgcca tccagccggg ttgcggagta aagattttct
    39721 gcttacgcaa ctgccagtaa agcagcgaag cattcagaca cgccgccaga ccaatagaaa
    39781 gtgacagccc ggcatgtttc aacggaccaa taaacgccag gttcatcaat tgcgttaaaa
    39841 tcagcgtaac gatggcaatt ttcactggcg ttttaatgtc ctggcgggaa taaaagccag
    39901 gagccaacac tttcactaca atcaggccaa tcaaacccac cgagtaggca attaacgccc
    39961 gctgggtcat cagcgcatca aacgcggtaa atttaccgta ctggaacagc gaaacggtta
    40021 acggacctga aagaatgccc aacgcaaccg cactcggcag cgccaacagg aaacaaagac
    40081 gcaatcccca gtccatcaaa cggttgtatt catcatgatt gccactggca aaacttttcg
    40141 acagcgacgg cagcaaaatg gtgccaagcg ccacgcccag cacaccggac ggaaactcca
    40201 ttaagcggtc ggcgtaatac atccaagaca ccgaaccgga agcaagaaac gaggcaaaaa
    40261 tggtgttgat gattaaggag atctggctca cagagacgcc aaggatcgcc ggtcccatct
    40321 gtttcaccac gcgcattgct ccggcatcgt ggaagttaat gcgcggcagg accagcatgc
    40381 cgatcttctt caggtgcggt agctgataca ccagctgcag gatgccgccg accgtaacag
    40441 cccacgccag cgccagcacc ggtgggttaa agtacggtgc ggcaaacagc gcgaaaccaa
    40501 tcatgctgat gttaagcagt gttggagcaa acgccggaat cgagaagcgg ttccacgtat
    40561 tcagaatcgc tcccaccagc gacgccaggg agatcagcaa gatataggga aaggtaatct
    40621 ttagtagctg gctggtcagg gcaaatttgt cagctgtgtc agcgaagcct ggcgcggtca
    40681 ccatgatcac ccacggtgcg gcgagcatgc cagcgaccgt cacaaccgcc agcgcgagtg
    40741 tcagcaggcc agaaacataa gagacaaaga cccgcgtggc gtcttcaccc tgcttacttt
    40801 tatattccgc cagaatcggt acaaatgcct gggaaaatgc cccttcggca aagatacggc
    40861 gtaacaagtt aggaagttta aaagcgacga aaaaggcgtc ggttgccatc cctgcgccaa
    40921 agattctggc gacaattgcg tctcgtgcga agccaagcac acgcgaaaac atggtcatcg
    40981 agctgacggc ggccagcgat tttaataaat tcatcggtgt tctaatccag acccacgggc
    41041 aaaacgcctg caaggcaggc gctgaaagag gcgaatagtc tacctggatt atggtgaatt
    41101 gctaccgcca gatgttacag ggtttattca ctcatcgcat cgcgccagat cttgtcaacg
    41161 atacgttgcg ccagcacggc ctgttcgccg gcggtttgcg gaactgtctg attttgcaca
    41221 cattcaatga agtgccgcgc acagccgaca aatccacgtt gttcaagagt gctctgccaa
    41281 ccaggaatcg gtttatgcac tacgccctgc ccgcgctcct cacgccattc gcgcatatcc
    41341 gtaatgtcga tgagcgcacc gtcagtcacg gcctgcacgg tttcacgctg acttccggca
    41401 cggcgatgca tacaggtggt gatctgcaaa ggaccagccg aaaaatggtg ctcggcaaac
    41461 agcatttcgc cagcgtcgtt agtcagtagc gtaccgccat ccagagaggc tttgccaccc
    41521 gacaaccaca gcgcggtatc caccacatgc agataatcat ccagcaacgt gaaataaaga
    41581 tcgtgtggcc cgacgctatt gctacgatgt ttatccattc ttaacgaggc tgcggtggcg
    41641 agttgcgttt ttaactctcc gtagagtggt gcgaaacgac ggttaaaacc gaccatcaac
    41701 gtcagttttt tccgcgccgc cagttccacc agccgttcag catcgcgcag attttctgcc
    41761 agcggtttat cgacacagac atgtaccccc gcattgagta acgtactgac cacgtcaaag
    41821 tggctggcgg tgctggaatg cacaaaaacc gcatcgcaac tggcggcaag gctggataac
    41881 gaatcggcat aaggaatgcg ccagctttca caaattggca gggctttcgc gcgcgtaggc
    41941 gaccaggctc cttgtaacat ccagtcagac gctgccgcca gcaccggtaa ccacgctttt
    42001 tgcgcaatgc cgcctaatcc cacaacgccg atacgtaatt ttttcacttt aatctcccag
    42061 atgggccagc aacgaatcaa gacgctgttt cagttctgcc acttcgattt ccagggcttc
    42121 gacgcgggcc tgtaaatcac catcaaccgc gttcgacata tccgttaccg ccggctgatc
    42181 ctctacctca ccactgaaaa gatgcatgta gcggctttcg cgtttacccg gttcgcgggc
    42241 cagacgcacc acaaaaggac catcttcgcg atttgccagt tgttccagcg tcgactccac
    42301 ttccgccata tcgctgaatt catacattcg cgcggcgcgg ctgcgcagtt cacctggcgt
    42361 ctgggcacca cgcaataaca acgtggtgat taacgccact tccgctgcgc tcagtttcag
    42421 atcgccaaat tctgaattac aaaaacgttg ctcatatttg gtgacccgat taccaaaacc
    42481 gctcactgtg cgtagataat gacgtttgac cagattatcc agctgttcct gcacttcgga
    42541 ttcactcaga ttcatgaccg gttcacggtt cgttttctga ttacaggccg tgactacacc
    42601 attgactgag agcgggtatt gctccggcgt ggtcacctgc ttttccagca agcaaccaat
    42661 cacgcgagct tcaagggcag taagttgata tttcatgaga ttctccttag cggccgggcg
    42721 tccagtctgg ggtagttaat gccgtcagta cgtgatcgcg ccattgtcca tcaatcaaca
    42781 gatagtcttt cgcatagcct tctttttcaa aacccagtcg cgccagtaaa tcaccgctgc
    42841 gtttattgtg cggcatataa ttagccataa tgcgatgaat atgttgggtg cgctgcatat
    42901 aacgaatggc tgcggtcagg gcttcaaaca tgagtccttt gccctgccat ttttgcccaa
    42961 tcgaataacc gagatagcag gcatgaaaag agccacgaac aacattggaa aaattggcaa
    43021 cgccaataat ctctttttcg tccgggtcga ataagccaaa gtagaaagct gaaccttgtt
    43081 tatgaaattc gttaatcatc cccagcctgg cctgccagcc tgatggataa cagtggcttt
    43141 cgtcgcgcac tggctcccag ggcttgagga aatggcgatt ctctgcgtaa taatccgcaa
    43201 gacgccaggc atcacgatca tgcaccagac gcacgaccag tcggtctgtg gttaagcgca
    43261 cttttggcac gttactgcga tagccaaaca ttctatacct actccttccc gtaacgtctt
    43321 taatacgctg taagtattac tatacctgcg caaccagacc ctgtgaaaag tccaaaatgc
    43381 cattttctgc attattcaca tatttgataa aaactctcta tcaaagcgac gttttgataa
    43441 aaaaatattg tccccgaagt gatgggaaaa gtcttagcgg caatgcagaa tagaaagtgc
    43501 tcatctttta tttcccggga ggggaaatgt cccgcgtatc gcaggcgagg aacctgggta
    43561 aatatttcct gctcatcgat aatatgctgg tcgtgctggg gttctttgtt gtcttcccgc
    43621 tgatctctat ccgcttcgtt gatcaaatgg gctgggccgc cgtcatggtc ggtattgctc
    43681 tcggtctacg ccagtttatt cagcaaggtc tgggtatttt cggcggcgca attgccgacc
    43741 gctttggtgc caaaccgatg attgttaccg gtatgctgat gcgcgccgcc ggattcgcca
    43801 caatgggtat cgcccacgaa ccgtggctat tgtggttttc atgcctgctc tcgggactcg
    43861 gcggcacgtt gtttgatccg ccgcgttcgg cgctggtggt gaaactaatc cgtccacaac
    43921 aacgtggtcg ttttttctcg ctgttgatga tgcaggacag tgccggtgcg gtcattggcg
    43981 cattgttggg gagctggctg ttgcaatacg actttcgcct ggtctgcgcc acaggggccg
    44041 ttttgtttgt gctgtgtgcg gcgttcaatg cgtggttgtt accggcatgg aaactctcca
    44101 ccgtacacac gcccgttcgc gaaggcatga cccgcgtgat gcgtgacaag cgttttgtca
    44161 cctatgtcct gacgctggcg ggttactaca tgctggctgt acaagtgatg ctgatgctgc
    44221 caattatggt caacgacgtg gctggcgcac cctctgccgt taaatggatg tatgccattg
    44281 aagcgtgtct gtcgttaacg ttgctctacc ctatcgcccg ctggagtgaa aagcattttc
    44341 gtctggaaca ccggttgatg gctgggctgt tgataatgtc attaagcatg atgccggtgg
    44401 gcatggtcag cggcctgcaa caacttttca ccctgatttg tctgttttat atcgggtcga
    44461 tcattgccga gcctgcgcgt gaaaccttaa gtgcttcgct ggcggacgca agagctcgcg
    44521 gcagctatat ggggtttagc cgtctgggtc tggcgattgg cggcgctatt ggttatatcg
    44581 gtggtggctg gctgtttgac ctgggcaaat cggcgcacca gccagagctt ccgtggatga
    44641 tgctgggaat tattggcatc ttcactttcc ttgcgctggg ttggcagttt agtcagaaac
    44701 gcgccgcgcg tcgtttgctg gaacgcgacg cctgatttga tactcccccc tgactcctcc
    44761 atactgaaag tgaactgaca gccaaatgga ggagtcacgt gaagctatac atttacgatc
    44821 actgccctta ctgcctcaaa gcccgcatga ttttcggcct gaaaaatatc cccgtcgaat
    44881 tacatgttct gctcaacgac gacgcagaaa cacccacccg gatggtcggt caaaaacagg
    44941 ttcccattct gcaaaaagat gacagccgct atatgccaga aagcatggat atcgttcact
    45001 atgtcgataa actcgacggc aaaccgttac tgaccggcaa acgttcccct gccattgaag
    45061 agtggctgcg caaggtcaat ggctacgcaa acaaactgct gttgccgcgt tttgccaaat
    45121 cggcatttga tgagttttct actcccgccg cgcgcaaata tttcgtcgac aagaaagagg
    45181 ccagcgcggg taattttgcc gacctgctgg cccactctga cggtctgatt aagaatatca
    45241 gcgatgattt gcgtgcgctg gacaaactga tcgtcaaacc gaacgccgtg aatggcgaac
    45301 tttcggaaga tgatattcag ctatttccgc tactgcgtaa tctgacgctg gtagccggaa
    45361 tcaactggcc aagccgcgtt gctgattacc gcgataatat ggcgaaacag acacaaatca
    45421 atttgttatc atcaatggcg atttaatcct gacccgccgc ggcagcctgt attcgcgggt
    45481 tgccgccgtt ttcagttttt tctgctggcg agtttatcgc tggtgacaca tgctgcgttg
    45541 tgtcaggata ttgacgcaga tttcatgtcg aggactacca tgaacaagtt tttatttgcc
    45601 gctgcattga tcgtcagtgg cctgctcgtt ggctgtaatc aactcaccca atacaccatc
    45661 accgaacaag aaattaacca gtcgcttgcg aaacataata atttctcaaa agatatcggt
    45721 ttacccggcg tggctgacgc ccatattgtt ctgacaaact tgaccagcca aattggtcgc
    45781 gaagagccga ataaggttac cctaaccgga gacgccaatc tgacatgaac tccctgttcg
    45841 gtagtcagaa agcgaccatg aaactgaagc tgaaagcgct gcccgtgttt gataaagaaa
    45901 aaggtgcgat cttcctgaaa gagatggagg tggtcgatgc gacggtacaa ccggaaaaaa
    45961 tgcaaacggt gatgcaaacg ttgcttccct atttgaacca ggcattacgc aattacttta
    46021 accagcaacc tgcttacgtc ctgcgcgaag atggcagtca gggcgaagca atggcgaaaa
    46081 aactggcgaa aggcattgaa gtgaagccag gcgaaattgt cattccgttt acggattaat
    46141 gacgagggcg cattcgcgcc ctttattttt cgtgcaaagg aaaacgtttc cgcttatcct
    46201 ttgtgtccgg caaaaacatc ccttcagccg gagcatagag attaatgact gcaccatccc
    46261 aggtattaaa gatccgccgc ccagacgact ggcaccttca cctccgcgat ggcgacatgt
    46321 taaaaactgt cgtgccgtat accagcgaaa tttatggacg ggctatcgta atgcccaatc
    46381 tggctccgcc cgtgaccaca gttgaggctg ccgtggcgta tcgccaacgt attcttgatg
    46441 ccgtacctgc cgggcacaat ttcaccccat tgatgacctg ttatttaaca gattcgctgg
    46501 atcctaatga gctggagcgc ggatttaacg aaggcgtgtt caccgctgca aaactttatc
    46561 cggcaaacgc aaccactaac tccagccacg gcgtgacgtc aattgacgca atcatgccgg
    46621 tacttgagcg catggaaaaa atcggtatgc cgctactggt gcatggtgaa gtgacacatg
    46681 cagatatcga catttttgat cgtgaagcgc gctttataga aagcgtgatg gaacctctgc
    46741 gccagcgcct gactgcgctg aaagtcgttt ttgagcacat caccaccaaa gatgctgccg
    46801 actatgtccg tgacggaaat gaacggctgg ctgccaccat cactccgcag catctgatgt
    46861 ttaaccgcaa ccatatgctg gttggtggcg tgcgtccgca cctgtattgt ttacccatcc
    46921 tcaaacgcaa tattcaccaa caggcattgc gtgaactggt cgccagcggt tttaatcgag
    46981 tattcctcgg tacggattct gcgccacatg cacgtcatcg caaagagagc agttgcggct
    47041 gcgcgggctg cttcaacgcc ccaaccgcgc tgggcagtta cgctaccgtc tttgaagaga
    47101 tgaatgctct gcagcacttt gaagcattct gttctgtaaa cggcccgcag ttctatggct
    47161 tgccggtcaa cgacacattc atcgaactgg tacgtgaaga gcaacaggtt gctgaaagca
    47221 tcgcactgac tgatgacacc ctggtgccat tcctcgccgg ggaaacggta cgctggtccg
    47281 ttaaacaata aaaatcgtag cttcctgttg tcattaggtt attttacctg tataaataac
    47341 cagtatattc aacagggggc tattatgcga attgaagtca ccatagcgaa aacttctcca
    47401 ttgccagctg gggctattga cgccctggct ggcgaacttt cccgccgtat tcagtatgcg
    47461 tttcctgata atgaaggcca cgtatcggta cgttatgccg cagcgaataa tttatcggtt
    47521 attggcgcga caaaagaaga taaacagcgc attagcgaaa ttctccagga aacgtgggaa
    47581 agcgccgatg actggtttgt cagcgaataa
//
LOCUS       DAZFQD010000018        47065 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.018, whole genome shotgun sequence.
ACCESSION   DAZFQD010000018 DAZFQD010000000
VERSION     DAZFQD010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 47065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47065
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.018"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(127..945)
                     /gene="cof"
                     /locus_tag="ACHKTW_001148"
     CDS             complement(127..945)
                     /gene="cof"
                     /locus_tag="ACHKTW_001148"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HFO6462210.1"
                     /translation="MARLAAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKEIPALLQAFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVLTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            1045..2745
                     /gene="ybaE"
                     /locus_tag="ACHKTW_001149"
     CDS             1045..2745
                     /gene="ybaE"
                     /locus_tag="ACHKTW_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
                     /protein_id="HFO6462211.1"
                     /translation="MRLLNRLNQYQRLWQPSAGKPQTVTVSELAERCFCSERHVRTLL
                     RQAQEAGWLEWQAQSGRGKRGQLRFLVTPESLRNAMMEQALETGKQQDVLELAQLAPG
                     ELRTLLQPFMGGQWQNDTPTLRIPYYRPLEPLQPGFLPGRAEQHLAGQIFSGLTRFDN
                     NTQRPIGDLAHHWETSTDGLRWDFYLRSTLHWHNGDAVKASHLHQRLLMLLQLPALDQ
                     LFISVKRIEVTHPQCLTFFLHRPDYWLAHRLASYCSHLAHPQFPLIGTGPFRLTQFTA
                     ELVRLESHDYYHLRHPLLKAVEYWITPPLFEKDLGNSCRHPVQITIGKPEELQRVSQV
                     SSGISLGFCYLTLRKSPRLSLWQARKVISIIHQSGLLQTLEVGENLITASHALLPGWT
                     IPYWQVPDEVKLPKTLTLVYHLPIELHTMAERLQATLAAEGCELTIIFHNAKNWDDTT
                     LLAHADLMMGDRLIGEAPEYTLEQWLRCDPLWPHVFDAPAYAHLQSTLDAVQVMPDEE
                     NRFNALKAVFSQLMADATLTPLFNYHYRISAPPGVNGVRLTPRGWFEFTEAWLPAPSQ
                     "
     gene            2810..3505
                     /gene="queC"
                     /locus_tag="ACHKTW_001150"
     CDS             2810..3505
                     /gene="queC"
                     /locus_tag="ACHKTW_001150"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HFO6462212.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLR"
     gene            complement(3557..3955)
                     /gene="fadM"
                     /locus_tag="ACHKTW_001151"
     CDS             complement(3557..3955)
                     /gene="fadM"
                     /locus_tag="ACHKTW_001151"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HFO6462213.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGLENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILSQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(4049..4420)
                     /gene="ybaV"
                     /locus_tag="ACHKTW_001152"
     CDS             complement(4049..4420)
                     /gene="ybaV"
                     /locus_tag="ACHKTW_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFO6462214.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAASVAKPTTVETKAEAPAA
                     QSKAAVPAKASDEEGTRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLVERNLAVLTL"
     gene            complement(4571..6442)
                     /gene="ppiD"
                     /locus_tag="ACHKTW_001153"
     CDS             complement(4571..6442)
                     /gene="ppiD"
                     /locus_tag="ACHKTW_001153"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFO6462215.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLDAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGAPG
                     INSDIITVDGDRAFVLRISEHKPEAVKPLADVQEQVKALVQHNKAQQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            complement(6634..6924)
                     /gene="hupB"
                     /locus_tag="ACHKTW_001154"
     CDS             complement(6634..6924)
                     /gene="hupB"
                     /locus_tag="ACHKTW_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013356995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-beta"
                     /protein_id="HFO6462216.1"
                     /translation="MKRKRRVNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKE
                     GDDVALVGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            complement(7115..9469)
                     /gene="lon"
                     /locus_tag="ACHKTW_001155"
     CDS             complement(7115..9469)
                     /gene="lon"
                     /locus_tag="ACHKTW_001155"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HFO6462217.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDAPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINSDNLHDYLGVQRFDYGRADNENRVGQVTGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            complement(9657..10931)
                     /gene="clpX"
                     /locus_tag="ACHKTW_001156"
     CDS             complement(9657..10931)
                     /gene="clpX"
                     /locus_tag="ACHKTW_001156"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HFO6462218.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            complement(11057..11680)
                     /gene="clpP"
                     /locus_tag="ACHKTW_001157"
     CDS             complement(11057..11680)
                     /gene="clpP"
                     /locus_tag="ACHKTW_001157"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFO6462219.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            complement(11926..13224)
                     /gene="tig"
                     /locus_tag="ACHKTW_001158"
     CDS             complement(11926..13224)
                     /gene="tig"
                     /locus_tag="ACHKTW_001158"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFO6462220.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            complement(13568..13885)
                     /gene="bolA"
                     /locus_tag="ACHKTW_001159"
     CDS             complement(13568..13885)
                     /gene="bolA"
                     /locus_tag="ACHKTW_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HFO6462221.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            14190..14768
                     /gene="yajG"
                     /locus_tag="ACHKTW_001160"
     CDS             14190..14768
                     /gene="yajG"
                     /locus_tag="ACHKTW_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFO6462222.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRDLRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            14812..16287
                     /gene="ampG"
                     /locus_tag="ACHKTW_001161"
     CDS             14812..16287
                     /gene="ampG"
                     /locus_tag="ACHKTW_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HFO6462223.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTEYPAGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
     gene            16749..17696
                     /gene="cyoA"
                     /locus_tag="ACHKTW_001162"
     CDS             16749..17696
                     /gene="cyoA"
                     /locus_tag="ACHKTW_001162"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit II"
                     /protein_id="HFO6462224.1"
                     /translation="MRLRKYNKSLGWLSLFAGTVLLSGCNSALLDPKGQIGLEQRSLI
                     LTAFGLMLIVVIPAILMAVGFAWKYRASNKDAKYSPNWSHSNKVEAVVWTVPILIIIF
                     LAVLTWKTTHALEPSKPLAHDEKPITIEVVSMDWKWFFIYPEQGIATVNEIAFPANTP
                     VYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHLIANEPGTYDGISASYSGPGFSGM
                     KFKAIATPDRAEFDQWVAKAKQSPNTMSDMAAFEKLAAPSEYNQVEYFSNVKPDLFAD
                     VINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHAESAH"
     gene            17718..19709
                     /gene="cyoB"
                     /locus_tag="ACHKTW_001163"
     CDS             17718..19709
                     /gene="cyoB"
                     /locus_tag="ACHKTW_001163"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
                     /protein_id="HFO6462225.1"
                     /translation="MFGKLSLDAVPFHEPIVMVTIAGIILGGLALVGLITYFGKWTYL
                     WKEWLTSVDHKRLGIMYIIVAIVMLLRGFADAIMMRSQQALASAGEAGFLPPHHYDQI
                     FTAHGVIMIFFVAMPFVIGLMNLVVPLQIGARDVAFPFLNNLSFWFTVVGVILVNVSL
                     GVGEFAQTGWLAYPPLSGIEYSPGVGVDYWIWSLQLSGIGTTLTGINFFVTILKMRAP
                     GMTMFKMPVFTWASLCANVLIIASFPILTVTVALLTLDRYLGTHFFTNDMGGNMMMYI
                     NLIWAWGHPEVYILILPVFGVFSEIAATFSRKRLFGYTSLVWATVCITVLSFIVWLHH
                     FFTMGAGANVNAFFGITTMIIAIPTGVKIFNWLFTMYQGRIVFHSAMLWTIGFIVTFS
                     VGGMTGVLLAVPGADFVLHNSLFLIAHFHNVIIGGVVFGCFAGMTYWWPKAFGFKLNE
                     TWGKRAFWFWIIGFFVAFMPLYALGFMGMTRRLSQQIDPQFHTMLMIAASGAVLIALG
                     ILCLVIQMYVSIRDRDQNRDLTGDPWGGRTLEWATSSPPPFYNFAVVPHVHERDAFWE
                     MKEKGEAYKKPDHYEEIHMPKNSGAGIVIAAFSTIFGFAMIWHIWWLAIVGFAGMIIT
                     WIVKSFDEDVDYYVPVAEIEKLENQHFDEITKAGLKNGN"
     gene            19699..20313
                     /gene="cyoC"
                     /locus_tag="ACHKTW_001164"
     CDS             19699..20313
                     /gene="cyoC"
                     /locus_tag="ACHKTW_001164"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HFO6462226.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            20313..20642
                     /gene="cyoD"
                     /locus_tag="ACHKTW_001165"
     CDS             20313..20642
                     /gene="cyoD"
                     /locus_tag="ACHKTW_001165"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HFO6462227.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            20654..21544
                     /gene="cyoE"
                     /locus_tag="ACHKTW_001166"
     CDS             20654..21544
                     /gene="cyoE"
                     /locus_tag="ACHKTW_001166"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HFO6462228.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGIAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPPVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            21729..22040
                     /locus_tag="ACHKTW_001167"
     CDS             21729..22040
                     /locus_tag="ACHKTW_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HFO6462229.1"
                     /translation="MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLS
                     AFILSAAMERAESVLDNQRRRELSNQSWELMNQLIAEPAQPTLALKALMKRKNSDGRQ
                     A"
     gene            22024..22566
                     /locus_tag="ACHKTW_001168"
     CDS             22024..22566
                     /locus_tag="ACHKTW_001168"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO6462230.1"
                     /translation="MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRAS
                     LKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEKQRVSGVLQGSQPSEIGVVRLVM
                     LGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
                     TPNAFGAVPMFLAIQHILAA"
     gene            complement(22622..23557)
                     /locus_tag="ACHKTW_001169"
     CDS             complement(22622..23557)
                     /locus_tag="ACHKTW_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFO6462231.1"
                     /translation="MLTKRILFSVMLIVSPSVVASEKAHELYDSIYGGKPAPDVINTL
                     HKMAESGDIDAQSLLGWEYYQPRYDTKPDVQEAIKWFELAAKQGDREAPLALGGIYYD
                     GEQVRVDYAKAYALFNQAAQHGVNLAWSRLGIMYANGQYVEVDCKKAKEYLDKGVHIY
                     GGPENFLATCRKDMIDRKTVDDTLPVITVTRSGMRDNFLDKGFSCMDSLFATTNKLGE
                     VANLRVTFSIRRPSGKEINQTVGFAPFGLNRLNISFTDYLFGSFTSNSSLILYKPEFE
                     RKSCATVRTTIVAATATINGKDVELLKAGAIEQKW"
     gene            23965..25329
                     /gene="yajR"
                     /locus_tag="ACHKTW_001170"
     CDS             23965..25329
                     /gene="yajR"
                     /locus_tag="ACHKTW_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO6462232.1"
                     /translation="MNDYKMTPGERRATWGLGTVFSLRMLGMFMVLPVLTTYGMALQG
                     ASEALIGIAIGIYGLTQAVFQIPFGLLSDRIGRKPLIVGGLAVFAAGSVIAALSDSIW
                     GIILGRALQGSGAIAAAVMALLSDLTREQNRTKAMAFIGVSFGITFAIAMVLGPIITH
                     KLGLHALFWMIAILATTGIALTIWVVPNSSTHVLNRESGMVKGSFSKVLAEPRLLKLN
                     FGIMCLHMLLMSTFVALPGQLADAGFPAAEHWKVYLATMLIAFGSVVPFIIYAEVKRK
                     MKQVFVFCVGLIVVAEIVLWNAQTQFWQLVVGVQLFFVAFNLMEALLPSLISKESPAG
                     YKGTAMGVYSTSQFLGVAIGGSLGGWIDGMFDGQGVFLAGAMLAAVWLAVASTMKEPP
                     YVSSLRIEIPADIAANEALKVRLLETEGIKEVLIAEEEHSAYVKIDSKVTNRFEIEQA
                     IRQA"
     gene            complement(25457..25948)
                     /gene="yajQ"
                     /locus_tag="ACHKTW_001171"
     CDS             complement(25457..25948)
                     /gene="yajQ"
                     /locus_tag="ACHKTW_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HFO6462233.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            26116..27027
                     /gene="panE"
                     /locus_tag="ACHKTW_001172"
     CDS             26116..27027
                     /gene="panE"
                     /locus_tag="ACHKTW_001172"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFO6462234.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVYDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            26990..27580
                     /gene="yajL"
                     /locus_tag="ACHKTW_001173"
     CDS             26990..27580
                     /gene="yajL"
                     /locus_tag="ACHKTW_001173"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HFO6462235.1"
                     /translation="MSASALVCLAPGSEETEAVTTIDLLVRGGIKVTTASVASDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGAECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(27634..29082)
                     /gene="thiI"
                     /locus_tag="ACHKTW_001174"
     CDS             complement(27634..29082)
                     /gene="thiI"
                     /locus_tag="ACHKTW_001174"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HFO6462236.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLAIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPEVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSIDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNR
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            29288..29530
                     /gene="xseB"
                     /locus_tag="ACHKTW_001175"
     CDS             29288..29530
                     /gene="xseB"
                     /locus_tag="ACHKTW_001175"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFO6462237.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            29530..30429
                     /gene="ispA"
                     /locus_tag="ACHKTW_001176"
     CDS             29530..30429
                     /gene="ispA"
                     /locus_tag="ACHKTW_001176"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HFO6462238.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDADMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQSLKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            30454..32316
                     /gene="dxs"
                     /locus_tag="ACHKTW_001177"
     CDS             30454..32316
                     /gene="dxs"
                     /locus_tag="ACHKTW_001177"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFO6462239.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSALRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLAI
                     LNFGTLMPEAAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            32496..33470
                     /gene="yajO"
                     /locus_tag="ACHKTW_001178"
     CDS             32496..33470
                     /gene="yajO"
                     /locus_tag="ACHKTW_001178"
                     /EC_number="1.17.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HFO6462240.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSIDDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALKLQKQHGWAQFISMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGRNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKPEQIAELETPYKPHAVVGFK"
     gene            complement(33524..34042)
                     /gene="pgpA"
                     /locus_tag="ACHKTW_001179"
     CDS             complement(33524..34042)
                     /gene="pgpA"
                     /locus_tag="ACHKTW_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFO6462241.1"
                     /translation="MTILPRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(34020..34997)
                     /gene="thiL"
                     /locus_tag="ACHKTW_001180"
     CDS             complement(34020..34997)
                     /gene="thiL"
                     /locus_tag="ACHKTW_001180"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HFO6462242.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLHDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTFDWKGYDHFATP"
     gene            complement(35075..35494)
                     /gene="nusB"
                     /locus_tag="ACHKTW_001181"
     CDS             complement(35075..35494)
                     /gene="nusB"
                     /locus_tag="ACHKTW_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFO6462243.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(35514..35984)
                     /gene="ribE"
                     /locus_tag="ACHKTW_001182"
     CDS             complement(35514..35984)
                     /gene="ribE"
                     /locus_tag="ACHKTW_001182"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HFO6462244.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(36073..37176)
                     /gene="ribD"
                     /locus_tag="ACHKTW_001183"
     CDS             complement(36073..37176)
                     /gene="ribD"
                     /locus_tag="ACHKTW_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HFO6462245.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVASMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRQRAQSHAILTSSATVLADDPALTVRWSELDEQTQAL
                     YPQQNLRQPVRIVIDSQNRVTPEHRIVQQPGETWFARTQEDSSEWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGALLQAGLVDELIVYIAPKLLGSDARGLC
                     TLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(37180..37629)
                     /gene="nrdR"
                     /locus_tag="ACHKTW_001184"
     CDS             complement(37180..37629)
                     /gene="nrdR"
                     /locus_tag="ACHKTW_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFO6462246.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDDVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            37780..38319
                     /gene="yajI"
                     /locus_tag="ACHKTW_001185"
     CDS             37780..38319
                     /gene="yajI"
                     /locus_tag="ACHKTW_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HFO6462247.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     KETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDADGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVEQLDFVRIHDIQPVMQ"
     gene            38618..39502
                     /gene="tsx"
                     /locus_tag="ACHKTW_001186"
     CDS             38618..39502
                     /gene="tsx"
                     /locus_tag="ACHKTW_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HFO6462248.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(39679..40026)
                     /gene="yajD"
                     /locus_tag="ACHKTW_001187"
     CDS             complement(39679..40026)
                     /gene="yajD"
                     /locus_tag="ACHKTW_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease YajD"
                     /protein_id="HFO6462249.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(40155..41126)
                     /gene="secF"
                     /locus_tag="ACHKTW_001188"
     CDS             complement(40155..41126)
                     /gene="secF"
                     /locus_tag="ACHKTW_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HFO6462250.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALAHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(41137..42984)
                     /gene="secD"
                     /locus_tag="ACHKTW_001189"
     CDS             complement(41137..42984)
                     /gene="secD"
                     /locus_tag="ACHKTW_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HFO6462251.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(43012..43344)
                     /gene="yajC"
                     /locus_tag="ACHKTW_001190"
     CDS             complement(43012..43344)
                     /gene="yajC"
                     /locus_tag="ACHKTW_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFO6462252.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(43367..44494)
                     /gene="tgt"
                     /locus_tag="ACHKTW_001191"
     CDS             complement(43367..44494)
                     /gene="tgt"
                     /locus_tag="ACHKTW_001191"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFO6462253.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSVYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(44550..45620)
                     /gene="queA"
                     /locus_tag="ACHKTW_001192"
     CDS             complement(44550..45620)
                     /gene="queA"
                     /locus_tag="ACHKTW_001192"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051075 - S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFO6462254.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDDRSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            45713..46294
                     /gene="acpH"
                     /locus_tag="ACHKTW_001193"
     CDS             45713..46294
                     /gene="acpH"
                     /locus_tag="ACHKTW_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008770 - [acyl-carrier-protein]
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HFO6462255.1"
                     /translation="MNFLAHLHLAHLAESSLSGNLLADFVRGNPEESFPPDVVAGIHM
                     HRRIDVLTDNLPEVREAREWFRSETRRVAPITLDVMWDHFLSRHWSQLSPDFPLQEFV
                     CYAREQVMTILPDSPPRFINLNNYLWSEQWLVRYRDMDFIQNVLNGMASRRPRLDALR
                     DSWYDLDAHYDALETRFWQFYPRMMAQASHKAL"
     gene            complement(46299..>47065)
                     /locus_tag="ACHKTW_001194"
     CDS             complement(46299..>47065)
                     /locus_tag="ACHKTW_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414937.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFO6462256.1"
                     /translation="MQHVAGITEAAKETQPEAYIVGEHFGDARQWLQADVEDAAMNYR
                     GFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDTARF
                     KTLLGRDIARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQDTAL
                     FALYQRMIALRKKSQALRRGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEACEVVLP
                     ASPFLNVAQWQRKEGHGQLTNGILALPAISATVWMN"
BASE COUNT        11136 a        12443 c        11659 g        11827 t
ORIGIN      
        1 cttttaattc tattttaaag gtgaaatcac gacaatttat tctgtgcggt ttaaacacca
       61 cggcacaaaa tgacaaattc aacgcaaaaa aaaccgggca attgcccggt gctggaaggg
      121 atctcgttat tcgggggaat aaggtagatg tggatagtcc agccagtgcg tcaaatagtg
      181 agagacagcc tgatttcggc aatgtccaat caccggtaaa tgcgggagct ccgcgcgcag
      241 ttgcggcatc gcattgccca taataaaccc gctaccgacg ctgcctaaca tttcgcgatc
      301 gttcatcgca tcaccaaagg ccatgcaatc gcgcaacgat aaacctaaat gttgggtcag
      361 caccgtcaat gcagcgcctt tattgcagcc caccggcagc acttcgaggc aatccgtggc
      421 ggaaaaacac aaatgtgcac gctcgcctaa tgcttcgtat agctggatct gcaagcgtgt
      481 aagatcgtcg tgatcgccac agaagcagat cttggtgacg ctgccgagtg gcattttttt
      541 gacatcgatt atctgataac gaaaaccgct atagacaaat gcctgcaaca acgcagggat
      601 ctctttcccg gtaaaccaac cgtcgtcatt gaagatatgc atgctggctc gggtatccca
      661 ttgctgatac agcaccagct ccgcgacatc cgcaggtaaa tcatcacgat gtaaaagttc
      721 accttccaga gaatgcacgc gcgttccgtt gccggtaatc aaatatgcat ccagcgatag
      781 cgccccgaga atatgctgca tctccagcgc atgacgcccc gtggcaaaag tgagggtaat
      841 gtcgcgttca cgcagtcgcg ccaaagtaga gagggttttc tcacctaaat gatggtcagg
      901 catcaataaa gtgccatcca tatcaaatgc tgccagacga gccatttctt tctccactct
      961 gtgacacggt taatggtgat tgagtatcac ctgatatata cggaagtaat agtgaatagt
     1021 taaataatat tgttccgggt ttatatgcga ttgctcaacc gtcttaacca gtatcaacgt
     1081 ctgtggcaac cttcagccgg aaagccgcaa accgtcaccg tcagcgaact ggccgaacgc
     1141 tgtttttgca gcgaacgcca tgttcgtacg ctgttgcgtc aggcacagga ggcgggatgg
     1201 ctggagtggc aggcgcagtc aggacgcgga aagcgcggac aattacgctt tctggtcacg
     1261 ccggaatcgc tacgcaatgc gatgatggaa caggcactgg aaaccggaaa gcagcaagat
     1321 gtgctggagc tggcgcaact ggccccaggt gagctgcgca ctctgttaca gccgtttatg
     1381 ggcggacaat ggcaaaacga tacacccacg ttgcgtattc cctactatcg cccgctcgaa
     1441 ccgctacaac caggcttttt gcccggccgt gccgagcagc atctcgccgg gcagatattt
     1501 tccggcctga cccgcttcga taataatact cagcgcccga ttggcgattt agcgcatcac
     1561 tgggaaacct ctactgacgg gttacgctgg gacttttatc ttcgttcaac cctacactgg
     1621 cataacggcg atgcagtaaa agcctcacac ttacaccagc gattattgat gctgttacaa
     1681 ctgccagcac tggatcaatt atttattagc gtgaagcgta ttgaagtcac ccatccgcag
     1741 tgtctgacct tctttttaca tcgccccgat tactggcttg cgcaccggct ggcgagctat
     1801 tgcagccatc tggcgcatcc gcaattccca ctgatcggca ctggtccttt tcgcttaaca
     1861 caattcacag cggagctggt gcgcctggag agccatgatt attaccattt gcgtcatccg
     1921 ctgcttaaag cggttgagta ctggataact ccgccgcttt tcgaaaaaga tttgggaaac
     1981 agttgtcggc atcccgtgca aatcaccatc ggcaaaccgg aggagctgca acgggtcagc
     2041 caggtcagta gtggcatcag tttaggtttt tgctatttaa cgttgcgcaa aagtccccga
     2101 ctctccctct ggcaggcgcg aaaagtgatc tccattattc atcaatccgg tttattacaa
     2161 acgttagaag tcggagaaaa cctgatcacc gccagtcatg cattactgcc aggctggact
     2221 attccgtact ggcaagtacc ggatgaagtc aaactaccga aaaccttgac gctggtttat
     2281 cacctaccga tagaacttca taccatggca gaacgcctac aggcgacact ggcagcggaa
     2341 ggctgtgaac tcacaattat ttttcataac gcaaaaaact gggacgacac gaccctactg
     2401 gcacacgcag acctcatgat gggcgacaga ttaattggcg aagcaccgga atatactctg
     2461 gagcaatggc tacgttgcga tccactatgg ccacatgttt tcgacgctcc agcatatgca
     2521 catctgcaat cgacgctgga cgcggtgcaa gtaatgcctg atgaggaaaa ccgatttaat
     2581 gccctgaaag cggtttttag ccagttaatg gcagacgcga cgctgacgcc gctgttcaac
     2641 tatcactatc gcattagtgc ccctcccggc gtgaacggtg tgcgactgac accgcgcggc
     2701 tggtttgaat ttaccgaagc ctggcttccc gcgccgtcgc aatgaatagc tggtccgggt
     2761 taatcgcagg cgttaccata acgcttttat tgttcaagca ggattatcta tgaaacgtgc
     2821 tgtcgttgtg ttcagtggag gccaggattc caccacctgt ctggtgcagg cattacaaca
     2881 atatgatgaa gtccattgcg tgacgttcga ttacggtcag cgacatcgcg cagaaatcga
     2941 cgtggcacgc gaactggcgc tgaaactggg agcacgcgcg cataaggttc ttgatgtaac
     3001 gctgctcaac gagctggcgg tcagcagcct gacacgtgac agcattccgg tgcctgatta
     3061 tgaacctgaa gccgatggca tcccgaatac gtttgtccca gggcgtaata ttttgttcct
     3121 gacgctggcg gcaatatatg cgtatcaggt aaaagcagaa gccgtaatta ccggcgtctg
     3181 tgaaacggat ttctccggct acccggattg ccgcgatgag tttgtgaaag cactaaacca
     3241 tgccgtcagt ttgggcatgg cgaaagatat tcgttttgaa acgccgctga tgtggattga
     3301 taaagcggaa acctgggcgc tggcagatta ttacggcaaa ctggatttag tccgtaacga
     3361 aacgttgacc tgctataacg gcattaaagg cgacggttgc ggtcattgtg cggcatgtaa
     3421 tttacgcgcc aacggtttga atcattatct ggccgataaa ccgacggtga tggcagcgat
     3481 gaagcagaaa accgggttga ggtaattatt ccgggtgtcg ccggatgcgg cttgagcatc
     3541 cggcaccaca aaacgtttac ttaaccatct gctccagctt ttcgcgcaat tccccttcca
     3601 gagctaatgc tttctgcgtt ttaagatcaa tacaaacaaa cgtaataagc gcatccgcta
     3661 ccacctgccc ttccggctcc agtgtaatga cctggcttaa gatgccgctt ttaccgttta
     3721 attgctgcaa ctgactggta atagttaaca ggtcacttaa taccgctggg cgacgatagt
     3781 taatattgat attgaccacg acgaaggcga tgttatgggc cgtcatccac tgaaaactgt
     3841 cgctattttc caacccatcc cagcgggctt cttcgagaaa ttcaaggtag cgggcgttgt
     3901 tgacgtgctg gtaaacgtcg agatgatatc cacgaacttt gatttgtgtt tgcatagcgc
     3961 aataacctta cgttgttgtt ataagtacag aggtcataac tggtatgacc tcttcagtct
     4021 ggcaaaaaat tgccactatg caaattaatt acagggttaa taccgccaga ttacgttcca
     4081 ccagcgaatt gcccatcccc ggcacctgct ttagatcctc caccgtttta aacggaccgt
     4141 actcttcgcg ataactgaca atcgcctgcg ctttcttcag gccaacgcca ttcatcgcgc
     4201 gggctagctc ttccgcgctg gcattattaa tgctgacccg ggtgccttct tcgtcactgg
     4261 ctttcgccgg tactgctgct ttactttgtg ctgcaggagc ttccgctttg gtttctaccg
     4321 ttgtcggttt cgccacagaa gctgccgcca gcgcgctatg agacattccg gcacaggcca
     4381 gggacagggt aatgagcagt gctttaattc cgtgtttcat actgttttct ccttgtttgt
     4441 taacagtgga atcaccttaa cggcagtgaa aacagtaaac aaatggcaat gttcagaaat
     4501 ggaaaaggcc gcgaaagcga ccttttcttg ttacagctgc aacgcatttt tttgcgaggc
     4561 ggcttcagga ttattgctgt tccagcgcat cgccaatttt gattttcgcc tctttacgca
     4621 ggttactcat cagagcttca aagacgattt gtgcgttgtt ctgggtgata ccctgcacca
     4681 tcgctttttt ctgatcttcc ggcattgaac cttgtttcac ttcatccagc gccagcagaa
     4741 ccacattacc ttgcatatcg gtcgccatac cgtagctcgg tttgtctttc gctggcagtg
     4801 gcagtgcaaa cgccgcctgg ctaatcgggt cacgaccgga acgacttaag gttttcggct
     4861 cgccaaattt cagaccggca gcctgcatag cttccgcacc tttgccggct ttcaaatcaa
     4921 ccagcagttt ctcagcatcc actttcgcct gttgttgagc tttgttgtgc tgaaccaatg
     4981 ccttaacttg ttcctgaaca tctgccaacg gtttcaccgc ttccggtttg tgctcgctga
     5041 tgcgcagcac gaatgcgcgg tcgccgtcta cggtgatgat gtcagagttg atgcccggcg
     5101 cgccgttttc acctaccaga ccgccgttaa agatagcgtc ggcaaccggc ttgaagttca
     5161 actcttccgg caggttatct ttgctgaacc aacccgtctg agtggcttta acgccagcag
     5221 cttgctctgc accggccaga gactcggtgt cgttgcttgc cgcatcgctc actttctgct
     5281 gcagcgcata gtacgcatcg agggcttttt cgtgtttcac tttcgccgca atgtcgtcac
     5341 gtacttcgtc taacgatttc actttcgctg gctgaatgtc gtccagacgt acaatcagga
     5401 aaccgaccga agatttgatg acaccagaca gttgcccttt ttctttcaga ccagcatttt
     5461 tcagttcgtc ggggatagtg gcatcttcta accaacccat atcgccgccg ttacgggcag
     5521 agataatatc ggcagatttt tctttggcta atgcagcaaa atcaccgcct ttattcagct
     5581 catcaagtac cgctttcgct tcatcttcag ttttggtctg gatgatgctg tagcgggtac
     5641 gctgcggctg ggtgaattga tcctgatgct gatcgtagta gctctggata tccgcatcgc
     5701 taaccggttg ctgcatcgtt gcggcatcca gcttgatgta actcacgcgg aattgttccg
     5761 gcgtcatgaa attgtttttg ttttgttcgt agtagctggc aatctcctgt tcggtcacag
     5821 gctgcttcgc cgccagcgcg ttaacatcga tagtcgcctc acgcaccacg cgttgttgcg
     5881 cgaccagtgc cgccagctcg tcggtttcac ctttcagcat aaaatcggta ccggcaacac
     5941 cgttaatcag ctgttgggta gtgagctggt tacgcagcgc ctgggcgtac tgatcggcgg
     6001 tcatccccat ctggttgagg ataccgttat agcggctgtt atcaaatttg ccgtcaacct
     6061 ggaaggctgg ggtcgcgaaa atcgcctgtt taacctgctc atcactgata cccagtttca
     6121 gctcacgagc gtactgatcc aacagcgcct cgtcgatcag acgattcagc acctgttgac
     6181 gcagggtttt catatagcct tcgttcgctg ccagctcgga gtattgatcg cccagctgtt
     6241 gctgcatgcg attacgctcg ctgttaaagg cgttctcgaa ctgcccacgg ctgatttcct
     6301 ggtcattcac ttttgcggcg tagttattgc ctccgccaat caggtaacca ctcacgccgg
     6361 tcaatatgaa cgacacgata atgataccga aaataatctt gagcacgaga ctgtttgcag
     6421 ccgtgcgtaa gctgtccatc atggtgtaac aacactccgc tgtagatgac tgtgtacctc
     6481 acgcaacgta tcgatgcgcg caaaggccta ttgtgacaag aaacgggggc aattgtcagc
     6541 ccacaagaca cagaaagtca ccgaatacaa ataaaaaagg cacatcagta gatgcgccct
     6601 tgaacttcgt cacatcccca ctggggacaa cgcttagttt accgcgtctt tcagtgcttt
     6661 acctgcacgg aagctcggta ctttagcggc agcgatggtg atctctttac cggtctgcgg
     6721 gttgcggcca gtacgggcag cacgctcttt aacggcaaaa gtaccaaaac ctaccagtgc
     6781 tacatcatcc ccttctttca gagattcagt tacggaagca ataatagcat ctaacgcacg
     6841 gccagccgca gctttagaga tatcagcccc tgcagcaatc ttgtcgatca attgagattt
     6901 attcactctt ctcttcctct ttataattta tatcgcactt gaatccttca aggtacgaac
     6961 gcgcagcagt tatatcaggc cagccatccc cttacaacac cccttaataa gggcaagccc
     7021 gatccgccat ctaacttagc gagacaaaaa aaggctggca agcccgaatt agcctgccag
     7081 ccctgttttt attagtgcat tttgcgcgag gtcactattt tgcagtcaca acctgcatgc
     7141 cagacggttc attttgcagc gccagagtca gaacttcctc aatgcgcttc acaggatgaa
     7201 tgtccagatc ggcaattacg ttgtcaggaa tctcttccag atcgcgttta ttttcgaacg
     7261 gaattagcac tgttttaatc ccgccgcgat gcgctgccag tagtttttct ttcaaaccac
     7321 cgatcggcag tacctgacca cgcagagtga tctcaccggt cattgccaca tcggcacgaa
     7381 ccgggttacc ggtcaggcaa gaaaccagcg cggtgcacat agcaataccg gcactcggac
     7441 catctttcgg cgtcgcacct tccggaacgt ggacgtggat gtcacgtttt tcgtaaaaat
     7501 cagggttgat ccccagtttt tccgcacgcg cacgaaccac cgttaacgcc gcctgaatag
     7561 actcctgcat cacttcgccg agcgaaccgg tataggtcag tttgccttta cccggaacac
     7621 atgcggtttc aatggtcagc aagtcaccgc ccacttccgt ccacgccaga ccggttacct
     7681 gaccgacacg gttttcgtta tcagcgcgac catagtcgaa acgctgaaca ccgaggtagt
     7741 catgcaggtt atcgctgtta atttcgatat gttttaatga cttatcgagc agtaactgct
     7801 taaccgcttt gcggcacagt ttggagattt cacgctccag accacgcacg cccgcctcac
     7861 gggtgtagta acgaataatg ccgataatgg cgctatcgtc gacggtcagc tcaccttttt
     7921 tcagtgcatt acgttcaatc tgcttcggca gcaggtgacg tttggcgatg ttcagttttt
     7981 catcttcggt ataaccggag aggcgaatca cttccatacg atccagcagc ggtgccggaa
     8041 tgttcatgga gttcgacgtc gcgacaaaca tcacgtcgct gagatcgtaa tccacttcca
     8101 ggtagtggtc gctgaacgct acgttctgct ctggatccag cacttcaagc agtgcagagg
     8161 ccggatcgcc tcgcatgtca gaagacattt tgtcgatctc atcgagcagg aacagcgggt
     8221 ttttcacgcc cactttcgcc attttctgga tcagtttacc cggcatagaa ccgatgtaag
     8281 tacggcggtg accacggatt tccgcttcat cacgcacgcc gcccagcgcc atacggacat
     8341 atttacgccc ggtggctttg gcaatggact gaccaagaga ggttttacct acccccggcg
     8401 gccctaccag gcagaggatc ggtcccttga ttttgttgac acggctttga accgcaaggt
     8461 actcaaggat gcgatctttc acgcgctcca gaccataatg gtcggtatca aggatttcct
     8521 gcgcctgacg caggtctttt ttgaccttgc tgcgcgcatt ccacggtacc tgtaccatcc
     8581 agtcgatata accacgcact acggtcgctt ctgccgacat cggagacatc attttcagct
     8641 tctgcaactc tgcttccgct ttctcttttg cctctttcgg catcttcgcc gcgtcgattt
     8701 tgcgcttcag ggcttcgttt tcgtccggcg cgtcgtccat ttcaccgagt tctttctgaa
     8761 tagctttcat ttgctcgttc agatagtact cacgctggga tttctccatc tgctttttaa
     8821 cgcggttgcg aatgcgtttc tcaacctgca gcagatcgat ttccgattcc atcattgcca
     8881 tcagatattc cagacgttcg ttaacgtcgg acatctccag aacggactgt ttgtcagcca
     8941 gtttcagcgg catatgtgca gcaatggtat ccgccagacg cgccggatcg tcgatgctat
     9001 tcagcgacgt cagcacttct ggtgggattt ttttgttcag cttgatgtag ccttcgaact
     9061 ggctgattgc agtacgcacc agcacttcct gttcccgctc atcaatggtc ggcgactcca
     9121 gatactccgc cttcgcagaa aagtgttcgc cattgtcaga gagcgcagaa atacgcgcgc
     9181 gctgtaaccc ctcgaccagc actttgacgg tgccgtcagg cagtttcagc atctgcaata
     9241 tagaggccac ggtcccgacg gtgaaaagat cgtttacacc cggctcatcc gttgaagctt
     9301 ctttctgcgc gaccagcata atttttttat catggtccat cgccgcttcc agacaacgga
     9361 tagatttttc ccgcccgaca aataagggga tgaccatgtg cggataaacc accacatcgc
     9421 gcagcggcaa tacggggatt tcaatgcgtt cagaacgctc aggattcata gagctctctc
     9481 ttagtttaat ttccgccagg taatcagatg acacgactgt gcttcacgcc atctattaac
     9541 atgtacgtca gtatatgggg atgtttcccc cacattcaac gccgagaata gaggaaaaat
     9601 taaaggggag ataaaatccc ccctttttgg ttaactaatt gtatgggaat ggttaattat
     9661 tcaccagatg cctgttgcgc ttccggctta ccataaatca gcaacggttt gctttgacca
     9721 tcaattaccg actcgtcgat aaccactttt tcgacatctt ccatggacgg cagatcgtac
     9781 atggtatcga gcagtgcggc ttctacgatg gaacgcaggc cacgggcacc ggttttacgc
     9841 gccatcgctt tcttagcgat agcatccagc gcctcgtcac ggaattccag atccacgcct
     9901 tccagattaa acagcgcctg atactgcttg gtcagggcgt ttttcggctc tttgaggatc
     9961 tgaatcagag cttcttcgct cagttcattc aacgttgcga caaccggcag acgaccaata
    10021 aactcaggga taagaccaaa cttgatcaga tcttccggtt caacctgcgc cagcagctcg
    10081 ccttcgcttg ctttgtcgga cttcgctttt accgtcgcgc caaaaccaat gccggagccg
    10141 gtttctacac ggtgggaaat cactttatcc agaccggcaa acgcaccgcc acaaataaac
    10201 aggatcttag aggtatcaac ctgcaagaat tcctgctgcg gatgtttacg cccaccttgc
    10261 ggtggaacag cagctaccgt accttcgatc agtttcaaca gtgcctgctg tacgccttca
    10321 ccggaaacgt ctcgggtaat ggacgggttg tctgacttac gagaaatctt gtcgatttca
    10381 tcgatgtaga caatcccgcg ctgcgctttc tgtacgtcgt aatcgcactt ctgcaacagc
    10441 ttctgaatga tgttttcaac gtcttcgccc acataaccgg cttcggtcag cgtggttgcg
    10501 tcggccatgg tgaacgggac gtccagcagg cgcgccagcg tttcagccag cagcgtttta
    10561 ccggaaccgg tcggaccgat cagcagaatg ttacttttgc ccaactcgac accattgctg
    10621 gtatcgccgt tgcgcagacg tttgtaatgg ttgtataccg cgaccgccag cacttttttc
    10681 gcctgttcct ggccgataac gtaatcgtcc aggtggttgc gaatttcatg cggcgtcggt
    10741 agcgcactgc gttcacgatg cggtgcaact tctttaatct cttcgcgaat gatgtcgtta
    10801 cataaatcaa cacattcgtc gcagatatac acggatggac cggcaatcag cttgcgcact
    10861 tcatgctggc ttttgccgca aaaagagcaa tacagcaatt tgcctgagcc atctttgcgt
    10921 ttatctgtca tgggtcaaaa cctcttcttt gttctttgtg ccgcacacga cgacgcaaat
    10981 gccattctca ggcgcaagcc gcttacagcg ttgtgccgcc ctggataagt atagcggcac
    11041 agttgcgcct ctggcatcaa ttacgatggg tcagaatcga atcgaccaga ccgtattcca
    11101 ccgcttcagg ggcggaaagg aagcgatcgc gctcggtatc acgttcaatc tgttctaatg
    11161 attgacccgt atgaagcgcc ataagttcat tcatgcgccc tttaactttc agaatttcac
    11221 gggcatgaat ttcgatatcg gtcgcctggc cctggtagcc gcccaacggt tggtgaatca
    11281 tcacgcgcga attcggcagg caaaaacgtt taccttttgc ccctgcggtc aacaagaaag
    11341 cgcccatcga ggccgcctgg cccatacaga tggtgctgac atcaggcttg ataaactgca
    11401 tggtgtcata gatagacatc ccggcagtga tcaccccgcc tggggagtta atgtacagat
    11461 agatatcttt ttctgggttt tccgcttcca ggaacagcat ctgcgccaca atcaggttag
    11521 ccatgtggtc ttcaacctgg ccagtcagaa aaatgacgcg ttccttaagt agacgagaat
    11581 agatatcaaa agagcgctca ccgcgtgagg tctgttcaat gaccatcggc accagcgcca
    11641 tatggggtgc aaagttatct cgttcgccac tgtatgacat ttccgtctcc tggataaaat
    11701 tgaaaaaacc tgctgtaccg attgtaacct tatggacgga ttatcagcta gtccctgtgt
    11761 tatgggtacg gcatcaccat atttcaagca taacaatctt ttgttgttac gctaacaccg
    11821 aaagaggctt ttcgcacggt tccatgcaaa attcatgaca aaaaaaagcc caccacctgg
    11881 cggtgacggg cctttgtgcg aatttagcgc gttatgctgc gtaaattacg cctgctggtt
    11941 catcagctcg ttgaaagtgg tttctttttc agtcactttc gctttcgcca gtacagcttc
    12001 aacagcctgt tcttccagag caacattgcg catgttgtcc atcagttctt tgtttttgct
    12061 gtagaactcg ataacttctt tcggatcttc gtacgcagaa gccatctctt cgatcaggcc
    12121 tttcacgcgc tcttcgtcag ctttcagctc gttggtgcgg ataacttcgc ccagcagcag
    12181 gccaacaact acgcggcgtt tagcctgttc ttcgaacagt tcgcgcggca gttccagagc
    12241 ttgtttttcg ttgccaccga aacgctgtgc agcctggcga cgcagaacgt cgatttcgct
    12301 gtcgatcagc gcagccggta cgtcgatgtc gttagctttt accagacctt cgatcgcctg
    12361 agacttaacg cggttacgga tggcgctctt cagctcgcgc tccatgtttt tacgcacttc
    12421 agcgcgcaga ccttctacgg aaccatcttc aacgccgaaa cgtttgatga attctgcagt
    12481 cagttccggc agttcacgct cttcaacttt cttcaggttg atagcgaatt tcgctgcttt
    12541 acctttcagg ttttctgcgt ggtattcttc cgggaaggtc acgtcgatgg tgaactcttc
    12601 gccagctttg tggcctttga taccgtcttc aaagcccggg atcatacgac cctggcccat
    12661 cgccagtacg aaatcagacg ctttaccgcc ttcgaactct tcgccgtcta cagaaccggt
    12721 gaagtcgatg gttacgcggt cttctgcttc aacagcgccg tctttttctt tccaggtcgc
    12781 ctgctgttta cgcagagtat ccagcatgcc gtcaacgtca gcgtcggtca cttcaacgat
    12841 cggtttttca acttcgatcg cttccagacc ctgcagttca acttccggat aaacttcaaa
    12901 ctctacagag taagtgaagt cttcacccag cttgtattcg cccggaacat aagtcggtgc
    12961 gccagccgga ttgatttttt ctttaatgat ggcgtcaatg aagttacggc tcatcaggtc
    13021 acccagaacg tcctggcgta cagacgcgcc ataacgctga gcaacgatat tcattggcac
    13081 tttgcctttg cggaagccgt caatacgtac ttttttcgca acgttgacca gctcgctttt
    13141 aacagcggtc tcgatgctgt cagcagcgat agtaatcgtt acacggcggc caaggccttg
    13201 agtggtttca actgaaactt gcatcttgtt acctcaaaaa atcacagtgc tcggtcaact
    13261 cttaccccgc aagcacaggt tgttggcttc gcggaaccgg gatgttctta taatcaatca
    13321 cattccctgt taccagaatc atcccgaaag cattcaaata ggacgcggca ttatagcggc
    13381 atcactttta tgagtcgaga acggttattg cgcgctgtcg cgtatttttt cacaattccc
    13441 gggcaatttt cgtctgaatg ctgggcaaaa agcgcaaaat ccagacaaaa caaaacggcc
    13501 cgcaggccgt ttttcataaa atctttagca acatactgga aaagcgccga cagttgcaaa
    13561 tgcgttttta cgcgatgctt cctgctccac gacagggagg agaggcaaag acggtgtcct
    13621 gcaacccttc ccactcctta atagtgtaag tatgcagagc cagcgcatga acggtagtag
    13681 agagttcctc cgctaaagta ctgtaaatca ttcgatgacg attcagaaaa cgttcacccg
    13741 taaaacgatc gctgaccagc acaactttaa aatggctttc agagccggct gggacattgt
    13801 gacgatagct ttcatccact acttcgagga atacgggttg gaacgccgcc cttaattttt
    13861 cttctatccg ctcacgtatc atcatgaaat atctcctccg acaacgctga gatgtcatcc
    13921 atccctttaa atactagccg cttttaccgt ttccattgca gcttaacaac aaatatttag
    13981 gtttcgtcct ggtttttccg tcaagcgtat gaagtttact gatttaaaga ttgaaacaca
    14041 ggcctcgcat cagcgtttct tttaccaggg gtgattattt cgtcaacatg atgaatttac
    14101 atgccttacc cacttcccct cgccgttggc aatgttatga tggcgggaat ttttaccaaa
    14161 cacgcttaag agtctctgag agcccgaaca tgttcaaaaa aatcctcttc ccgttagttg
    14221 ctctgtttat gcttgcagga tgcgcaaaac cgccaacaac tattgaagtt tccccgacga
    14281 ttacactgcc acagcaggat ccaagcctga tgggcgtcac cgtaagcatt aatggtgccg
    14341 atcagcgtac cgatcaggcg ctggcaaaag tcacccgcga taatcaaatc gttaccctga
    14401 ccgcctcccg cgatctgcgt ttcctgctgc aagaagtgct ggaaaaacag atgaccgcgc
    14461 gtggttacat ggttggtccg aatggcccgg ttaatctgca aatcatcgtt agccaactgt
    14521 atgctgacgt gtcccagggc aacgtgcgct ataacatcgc gaccaaagcg gatatcgcca
    14581 tcatcgctac cgcgcagaat ggcaacaaaa tgaccaaaaa ctatcgtgcc agctacaacg
    14641 ttgaaggtgc gttccaggcc tccaacaaaa atatcgccga tgcggttaac agtgtgctga
    14701 ccgataccat cgctgatatg tctcaggaca ccagcattca cgaatttatc aagcagaacg
    14761 cgcgttaatt tctgccctct ggcccggtgc aaaccgggcc tgtagacgcc catgtccagt
    14821 caatatttac gtatttttca acagccgcgt tcagccatat tgctgatcct gggttttgct
    14881 tccgggctac cgctcgccct gacatccggc accttacagg cctggatgac ggtcgagaat
    14941 attgatctca aaaccattgg tttcttctct ctggtaggcc aggcttacgt ttttaaattc
    15001 ctctggtcac cgctgatgga ccgctacacg cccccatttt ttgggcggcg gcgcggttgg
    15061 ctgctcgcca cgcaaatcct gttattagtc gccattgcgg cgatgggttt tctcgaacct
    15121 ggcacccaac tccgctggat ggcggcactg gcggtggtga tcgctttttg ctcggcctcc
    15181 caggatattg tcttcgatgc gtggaaaacc gatgtgctcc cggcagaaga acgtggtgcg
    15241 ggcgcggcaa tcagcgtgct gggttaccgt ttagggatgc tggtttccgg cggcttagcc
    15301 ctgtggctgg cagataaatg gctgggctgg cagggcatgt actggttgat ggcggcgctg
    15361 ttgatccctt gtattatcgc gacgttgctt gcaccagaac cgaccgacac cattcctgtg
    15421 ccaaaaacgc tggaacaagc ggttgttgca cctctgcgag atttctttgg tcgcaataat
    15481 gcctggctta ttttgcttct tatcgtgctg tataagctgg gcgacgcatt cgccatgagc
    15541 ctgacaacca cgtttttgat tcgcggcgtc gggtttgatg cgggtgaagt aggcgtggtt
    15601 aacaaaacgc ttggcttact ggcgaccatt gtgggcgcat tgtacggtgg gattttgatg
    15661 cagcgcctgt cgctgttccg ggcgctgctg attttcggca ttttacaagg tgcgtctaac
    15721 gctggttact ggctgctgtc gattactgat aagcatctct acagcatggg cgcagccgtc
    15781 tttttcgaaa acctctgtgg cgggatgggc acatcagcct ttgtcgcgct gttaatgacg
    15841 ctgtgtaata agtcattttc cgcaactcaa tttgccctgc tctcagcgct ttccgctgta
    15901 gggcgagttt atgtcggccc cgtggcgggt tggtttgttg aagcacacgg ctggtcgaca
    15961 ttctatctat tctccgtcgc cgctgccgta ccggggctta ttttgctgct ggtttgccgc
    16021 cagacgcttg aatatacacg agtaaatgac aactttatct cccgtaccga atatccggca
    16081 ggttatgcct ttgccatgtg gacactggcg gcgggcgtca gcctgttggc cgtgtggtta
    16141 ctgctgttga cgatggacgc gctggatttg acgcacttct ctttcctgcc tgctctgctg
    16201 gaagtcgggg ttttagtcgc cctttctggc gtcgtgcttg gtggtttgct ggattatctg
    16261 gcgctacgaa aaacgcatct gacgtaatct gtaaatatta tttagctgta gtaatcactc
    16321 gccgtaatta ttacggctaa ataaacatca gtatcgctta ataatatttg ttcttttgtg
    16381 cggcttagcg tttggaaatt attgacgcaa tttatgaatt ctattttttc ttacacgatt
    16441 cagctaatga gtctttattt tcccccccac ccaattgtca ccataatgat aattacttgt
    16501 taaacaattg ttttattcca gattggttat accaattgcc cgtccaaact ccttctgcac
    16561 tcccctttcg ttataacgcc cttttgcaac agcttcttaa aatcaacttg atatgttttg
    16621 caacatatgt gacctgacag ccaaatccaa gtaacaggaa tttaatcatg tttacagtaa
    16681 tgtaaccttc ccgtaaaatg cccacacact ttaaacgcca ccagatcccg tggaattgag
    16741 gtcgttaaat gagactcagg aaatacaata aaagtttggg atggttgtca ttatttgcag
    16801 gcactgtatt gctcagtggc tgtaattctg cgctgttaga tcccaaagga cagattggtc
    16861 tggagcaacg ttcactgata ctgacggcat ttggcctgat gttgattgtc gttattcccg
    16921 caatcttgat ggctgttggt ttcgcctgga agtatcgtgc gagcaataaa gatgctaagt
    16981 acagcccgaa ctggtcacac tccaataaag tggaagctgt ggtctggacg gtacctatct
    17041 taatcatcat cttccttgcg gtactgacct ggaaaaccac tcacgctctt gagcctagca
    17101 agccgctggc acacgacgag aagcccatta ccatcgaagt ggtttccatg gactggaaat
    17161 ggttcttcat ctacccggaa cagggcattg ctaccgtgaa tgaaatcgct ttcccggcga
    17221 acactccggt gtacttcaaa gtgacctcca actccgtgat gaactccttc ttcattccgc
    17281 gtctgggtag ccagatttat gccatggccg gtatgcagac tcgcctgcat ctgatcgcca
    17341 acgaacccgg tacttatgac ggtatctccg ccagctacag cgggccgggc ttctcaggca
    17401 tgaagttcaa agctattgca acaccggatc gcgccgaatt cgaccaatgg gtcgcaaaag
    17461 cgaaacagtc gccgaacacc atgtctgaca tggctgcgtt cgaaaaactg gccgcgccta
    17521 gcgaatacaa ccaggtggaa tatttctcca acgtgaaacc agacttgttt gccgatgtga
    17581 ttaacaagtt tatggctcac ggtaagagca tggacatgac ccagccagaa ggtgagcaca
    17641 gtgcacacga aggtatggaa ggcatggaca tgagccacgc ggaatccgcc cattaaaggg
    17701 gttgaggaag aataaagatg ttcggaaaat tatcacttga tgcagtcccg ttccatgaac
    17761 ctatcgtcat ggttacgatc gctggcatta ttttgggagg tctggcgctc gttggcctga
    17821 tcacttactt cggtaagtgg acctacctgt ggaaagagtg gctgacctcc gtcgaccata
    17881 aacgcctcgg tatcatgtat atcatcgtgg cgattgtgat gttgctgcgt ggttttgctg
    17941 acgccattat gatgcgtagc cagcaggctc ttgcctcggc gggcgaagcg ggcttcctgc
    18001 cacctcacca ctacgatcag atcttcaccg cgcacggcgt gattatgatc ttcttcgtgg
    18061 cgatgccttt cgttatcggt ctgatgaacc tggtggttcc gctgcagatc ggcgcgcgtg
    18121 acgttgcgtt cccgttcctc aacaacttaa gcttctggtt tactgttgtt ggtgtgattc
    18181 tggttaacgt ttctctcggc gtgggcgaat ttgcgcagac cggctggctg gcctatccac
    18241 cgctatcggg aatagagtac agtccgggag tcggtgtcga ttactggata tggagtctcc
    18301 agctatccgg tataggtacg acgcttaccg gtatcaactt cttcgttacc attctgaaga
    18361 tgcgcgcacc gggcatgacc atgttcaaga tgccagtatt tacctgggca tcactgtgcg
    18421 cgaacgtact gattattgct tccttcccaa ttctgacggt taccgtcgcg ttgttgaccc
    18481 tggatcgcta tctgggcacc catttcttta ccaacgatat gggtggcaac atgatgatgt
    18541 acatcaacct gatttgggcc tggggccacc cggaagttta catcctgatc ctgcctgttt
    18601 tcggtgtgtt ctccgaaatt gcggcaacct tctcgcgtaa acgtctgttt ggttatacct
    18661 cgctggtatg ggcaaccgtc tgtatcaccg tgctgtcgtt catcgtttgg ctgcaccact
    18721 tctttacgat gggtgcgggc gcgaacgtaa acgccttctt tggtatcacc acaatgatta
    18781 tcgccatccc gaccggggtg aagatcttca actggctgtt caccatgtat cagggccgca
    18841 tcgtgttcca ttctgcgatg ctgtggacca tcggttttat cgtcactttc tcggtgggcg
    18901 ggatgactgg cgtgctgctg gcagtaccgg gcgcggactt cgttctgcat aacagcctgt
    18961 tcctgattgc gcacttccat aacgtgatca tcggcggcgt ggtcttcggc tgcttcgcag
    19021 ggatgaccta ctggtggcct aaagccttcg gtttcaaact gaatgaaacc tggggtaaac
    19081 gcgcgttctg gttctggatc atcggcttct tcgttgcctt tatgccgctg tatgcgctgg
    19141 gcttcatggg catgacccgt cgtttgagcc agcagattga cccgcagttc cacaccatgc
    19201 tgatgattgc agctagcggt gcagtactga ttgcgctggg tattctctgc ctcgttattc
    19261 agatgtacgt ttctattcgc gaccgcgacc agaaccgtga cctgactggc gacccgtggg
    19321 gtggccgtac gctggagtgg gcaacctctt ccccgcctcc gttctataac tttgccgttg
    19381 tgccgcacgt tcacgagcgt gatgcattct gggaaatgaa agagaaaggc gaagcgtata
    19441 aaaagcctga ccactatgaa gaaattcata tgccgaaaaa cagcggtgca ggtatcgtca
    19501 ttgcagcttt ctccaccatc ttcggtttcg ccatgatctg gcatatctgg tggctggcga
    19561 ttgttggctt cgcaggcatg atcatcacct ggatcgtgaa aagcttcgac gaggacgtgg
    19621 attactacgt gccggtggca gaaatcgaaa aactggaaaa ccagcatttc gatgagatta
    19681 ctaaggcagg gctgaaaaat ggcaactgat actttgacgc acgcgactgc ccacgcgcac
    19741 gaacacgggc accacgatgc aggcggaacc aaaatcttcg gattttggat ctacctgatg
    19801 agcgactgca ttctgttctc aatcttgttt gctacctatg ccgttctggt gaacggcacc
    19861 gcaggcggcc cgacaggtaa ggacattttc gaactgccgt tcgttctggt tgaaactttc
    19921 ttgctgttgt tcagctccat cacctacggc atggcggcta tcgccatgta caaaaacaac
    19981 aaaagccagg ttatctcctg gctggcgttg acctggttgt ttggtgccgg atttatcggg
    20041 atggaaatct acgaattcca tcacctgatt gttaacggca tgggtccgga tcgcagcggc
    20101 ttcctgtcag cgttctttgc gctggtcggc acgcacggtc tgcacgtcac ttccggtctt
    20161 atctggatgg cggtgctgat ggtacaaatc gctcgtcgcg gcctgaccag cactaaccgt
    20221 acccgcatca tgtgcctgag cctgttctgg cacttcctgg atgtggtttg gatctgtgtg
    20281 ttcactgttg tttatctgat gggggcgatg taatgagtca ttctaccgat cacagcggcg
    20341 cgtcccatgg cagcgtaaaa acctacatga caggctttat cctgtcgatc attctgacgg
    20401 tgattccgtt ctggatggtg atgacagggg ctgcctctcc ggccgtaatt ctgggaacaa
    20461 tcctggcaat ggcagtggta caggttctgg tgcatctggt gtgcttcctg cacatgaata
    20521 ccaaatcaga tgaaggctgg aacatgacgg cgtttgtctt caccgtgcta atcatcgcta
    20581 tcctggttgt aggctccatc tggattatgt ggaacctcaa ctacaacatg atgatgcact
    20641 aagagcggcg gttatgatgt ttaagcaata cctgcaagta acgaaaccag gcatcatctt
    20701 tggcaacctg atctcggtga ttgggggatt cctgctggcc tcaaagggca gcattgatta
    20761 tcccctgttt atctacacgc tggttggggt gtcactggtt gtggcgtcgg gttgtgtgtt
    20821 taacaactac atcgacaggg atatcgacag aaagatggaa aggacgaaga atcgggtgct
    20881 ggttaaaggc ctgatctctc ctgctgtctc gctggtgtac gccaccttgc tgggtattgc
    20941 tggctttatg ctgctgtggt ttggcgcgaa tccgctggcc tgctggctgg gggtgatggg
    21001 ctttgtggtt tatgtcggcg tctatagcct gtacatgaaa cgccactcag tctacggcac
    21061 attgattggt tcgctctccg gcgctgcgcc gccggtgatt ggctactgtg cagtaaccgg
    21121 cgagttcgat agcggcgcag cgatcctgct ggctatcttc agcctgtggc agatgcctca
    21181 ctcctatgcc atcgccattt tccgctttaa ggattaccag gcggcaaaca ttccggtatt
    21241 gccagtggta aaaggcattt cggtggcgaa gaatcacatc acgctgtata tcatcgcctt
    21301 tgccgttgcc acgctgatgc tctctcttgg cggttacgct ggatataaat atctggtggt
    21361 cgccgcggcg gttagcgtct ggtggttagg tatggctctg cgcggttata aagttgctga
    21421 tgacagaatc tgggcgcgca agctgttcgg cttctctatc atcgccatca ctgccctctc
    21481 ggtgatgatg tccgttgatt ttatggtacc ggactcgcat acgctgctgg ctgctgtgtg
    21541 gtaacaaaac ctctctatta aaaaggtgct acggcacctt ttttattgtc tgagatcaga
    21601 gatacggcta ataaccgtat gtcttgacag agtgagcaga tgaacataaa ttaatcatac
    21661 ggttgatagc cgtattatgt atgagatcga ctccctacga tttcattcag ttaatcaaag
    21721 aggtcataat gcctggaaaa acagccactc tacccgatgt cgataaaacg ctgaaaaatg
    21781 cgcgggtcga actcaaaact agtcccgacg ccaaaaacaa acttcgtgaa gctgcgcaag
    21841 ccgttggggt agatttaagc gcttttattt tgagcgctgc tatggaacgc gccgaaagtg
    21901 tgcttgataa ccaacgtcgc cgtgagcttt cgaatcaaag ctgggaactg atgaaccaac
    21961 tcatcgctga acctgctcaa ccgacgctcg ccctcaaggc gttaatgaaa aggaaaaaca
    22021 gcgatggtcg acaagcatga agagattact ctgcccatag tcctctcctg taattatcag
    22081 tctgatatta cttatcctgg gcaaaaacag tttgattgcg gtaaccctgt tatcgataaa
    22141 tttgtacgcg catcgctaaa gaaaagtgtg cgtaatagcg actgtgcggc taaagcactt
    22201 attgacagac aaagtggtga actgatcggc atctgtactt ttacggcata ttcgctggaa
    22261 aaacaacgcg tttctggcgt ccttcagggt tcacaacctt cagaaattgg tgttgtcaga
    22321 ttagtcatgt tgggggtagc acggaagtat caaaagcggg gctttggtca ggacctacta
    22381 tgtgattttt ttgaacatgt aaaaataatt caccaggcat taccaattaa aggggtttat
    22441 cttgatgctg accctgccgc cattaatttt tatgctcgtc tcggctttgt tcagctttca
    22501 gcgacaccaa atgcttttgg tgctgtacct atgtttttgg cgattcagca tattctcgcg
    22561 gcttagatta ttatcgccac ccgccagggc aagttttgcc tcagccgggg gctgagcagc
    22621 gttaccactt ttgttcaatt gcccccgctt tcagcaactc cacatccttg ccgttaatgg
    22681 ttgccgttgc ggcaacgatt gtggtcctta cagttgcaca agattttcgc tcaaattccg
    22741 gtttataaag aataagagac gaattactgg taaatgaacc aaataggtaa tcagtaaagc
    22801 taatattcag ccggtttaaa ccgaaaggcg cgaagccaac cgtttggtta atctccttac
    22861 cggatgggcg acgaatgctg aatgtcacgc gaagattggc cacttcgcct aatttattgg
    22921 tggtggcgaa gaggctatcc atgcaggaaa aacctttatc taaaaaatta tctcgcatcc
    22981 cagaacgggt aaccgtaatc acgggtaacg tatcgtcaac cgtttttctg tcaatcatgt
    23041 cttttcgaca agtagccaga aagttttctg ggccaccata aatgtggaca cctttatcaa
    23101 gatattcctt cgctttctta caatctacct caacatactg accattggcg tacataatgc
    23161 ccaacctgga ccatgctaaa ttcacaccgt gctgcgcggc ctgattaaat agtgcatacg
    23221 ctttggcata atccacccgc acctgctcac cgtcgtagta gatacccccc agcgctaacg
    23281 gagcttctct gtcaccttgt ttagccgcta actcgaacca tttaatcgct tcctgaacat
    23341 caggtttggt atcataacga ggctgataat attcccagcc taacaggctc tgcgcgtcga
    23401 tatctcccga ttcagccatt ttatgcagtg tatttataac atcgggagca ggtttaccac
    23461 cgtatatgct gtcatacaat tcatgcgctt tctcgctggc caccacgctt ggtgaaacta
    23521 tcagcattac agaaaataat attcttttag ttaacatgta tacctttatg tcctcactgt
    23581 catatatttc cataataatt aaccaccaaa catccattta atgaataaat acagtgccat
    23641 aaaggtccaa gtaataagca cacatttagc ctgaaacatt atcatccatg tcaccaaatc
    23701 gtttatttag tgagataaaa ctagcatatt cgtaagataa aaaatgcaca tagcaatcaa
    23761 gaaatgatat tattactttt ttgatgtaaa actatttagt tttaaaattc attttttcaa
    23821 catatagagc attcatttta tgaatgcaaa aaaacatacc tgacacacaa attactctcc
    23881 tctgtaatat tttctcatta acactctgtt tacccaacat gcgctccgca ctacactatg
    23941 tgggcatttt tttaaggggt ggtaatgaac gattataaaa tgacgccagg tgagaggcgc
    24001 gcgacctggg gtttagggac cgtattctcg ttgcgcatgc tgggcatgtt tatggttctg
    24061 ccggttctga ccacgtacgg catggctctg caaggtgcca gcgaagcatt aatcggtatt
    24121 gccattggta tttatggtct gactcaggcc gtttttcaga ttccgtttgg cctgctttcc
    24181 gaccgcattg gtcgcaaacc attaattgtc ggtgggctgg cggtgtttgc cgccggtagc
    24241 gttatcgctg cgctttccga ctccatctgg ggaattattc tgggccgggc gctacaaggc
    24301 tctggagcaa ttgccgccgc cgttatggcg ctgctttccg atctcacgcg cgaacaaaac
    24361 cgcaccaaag cgatggcgtt tatcggcgtg agctttggca ttacctttgc cattgcgatg
    24421 gtgcttggcc cgatcatcac tcacaaactt gggctgcacg cgctgttctg gatgatcgct
    24481 attctggcaa caaccggcat tgcgttgacc atttgggttg tgcccaacag tagcactcac
    24541 gtacttaatc gtgagtccgg aatggtgaaa ggcagtttca gtaaagtgct ggcggaaccg
    24601 cggctgctaa aactcaactt tggcattatg tgtctgcaca tgcttctgat gtctacgttt
    24661 gttgccctgc ccggacagct agctgatgcg gggttcccgg cggctgaaca ctggaaggtc
    24721 tatctggcga cgatgttaat cgcctttggc tcggtcgtgc ctttcattat ctacgctgaa
    24781 gttaagcgca aaatgaagca agtctttgtc ttctgcgtcg ggttgatcgt ggttgcggaa
    24841 attgtgttgt ggaacgcaca aacgcagttc tggcaactgg tggtcggcgt gcagcttttc
    24901 tttgtggcgt ttaatttgat ggaagccctt ctgccctcac ttatcagtaa agagtcgcca
    24961 gcaggttaca aaggtacggc gatgggtgtc tactccacca gccagtttct tggcgtggcg
    25021 attggcggtt cgctgggcgg ctggattgac ggcatgtttg acggtcaggg ggtatttctc
    25081 gctggcgcaa tgctggccgc agtgtggctg gcagtcgcca gtaccatgaa agaaccgccg
    25141 tatgtcagca gtttgcgcat tgaaatcccg gcggacattg ccgcaaacga agcgttaaaa
    25201 gtgcgtttgc tggaaactga aggcatcaaa gaagtgttga ttgcagaaga agaacattca
    25261 gcttatgtga aaatcgacag caaagtgacg aatcgctttg agatagaaca ggcaattcgt
    25321 caggcataaa aaacgcggcg aattgcctgc tgcgctacgc ttatcaggcc tacaaagttt
    25381 ctgaattaca aacctttgta ggccgaatca ggcgttcatg ccgcatctga catgaacaaa
    25441 acgcacatag tcgcgattaa tcgcggaagt ttttgaactg gaacggctga ccgagatcgc
    25501 caccacgtac catcgccatg acagcctgca aatcatcacg agatttgccc gttacgcgga
    25561 tttcatcgcc ctgaatttgc gcctgcactt tcagtttgct gtctttgatc atcttgacga
    25621 ttttcttctg ggtcgcgctc tcaatgccct gtttcagttt cgcttccaca aaccaggttt
    25681 taccgctatg aacgatattc tccggtacat ccagcgaact gccttcaatg ccgcgcttca
    25741 gcagcttggc acgcagaata tccagcaact gattgacctg gaaatcggac tcgctcaaca
    25801 ctttgatggt tttgctggcg tcgttcagct caaatgaggc ttcaacgtta cggaagtcaa
    25861 aacgggactc cacttcgcgg ctcgcgttat cgaccgcgtt acgtgcttcc tgaagatcaa
    25921 cttcagagac aatatcgaaa gatggcatct tttcctctcc cttcattttt gatgcgaagc
    25981 ataatacccg caaagttaaa taaccgacag tcctgcacca ggacgtatcc cttaattgtg
    26041 catagtcgca acagcatgca caattaaggg atagccttat tgaacacccg gagtggttgc
    26101 gggtgaggag gaacaatgaa aattaccgta ttgggatgcg gtgccttagg gcaattatgg
    26161 cttacagcac tttgcaaaca gggtcatgaa gttcagggct ggctgcgcgt accgcaacct
    26221 tattgtagcg tgaatctggt tgagacagat ggttcgatat ttaacgaatc gctgaccgcc
    26281 aacgatcccg attttcttgc caccagcgat ctgctcctgg tgacgctgaa agcatggcag
    26341 gtttacgatg ccgtcaaaag cctcgcgtcc acactgcctg taaccacgcc aatactgtta
    26401 attcacaacg gcatgggcac catcgaagag ttgcaaaaca ttcagcagcc attactgatg
    26461 ggcaccacca cccatgccgc ccgccgcgac ggcaatgtca ttattcatgt ggcaaacgga
    26521 atcacgcata ttggtccggc acggcaacag gatggcgatt acagttatct ggcggatatt
    26581 ttgcaaaccg tgttgcctga cgttgcctgg cataacaata ttcgcgccga gctgtggcgc
    26641 aagctggccg tcaactgtgt gattaatcca ctgaccgcca tctggaattg cccgaatggt
    26701 gaattacgtc atcatccgca agaaattatg cagatatgcg aagaagtcgc ggcagtgatc
    26761 gagcgcgaag ggcatcatac ttcagcagaa gatttgcgtg attacgtgat gcaggtgatt
    26821 gatgccacag cggaaaatat ctcgtcgatg ttgcaggata tccgcgcgct gcgccacacc
    26881 gaaatcgact atatcaatgg ttttctctta cgccgcgccc gcgcgcatgg gattgccgta
    26941 ccggaaaaca cccgcctgtt tgaaatggta aaaagaaagg agagtgaata tgagcgcatc
    27001 ggcactggtt tgcctcgccc ctggtagtga agagactgaa gccgtcacca ctatcgatct
    27061 gctggttcgc ggcggtatca aagtcaccac tgccagcgtc gccagcgatg gcaacctggc
    27121 cattacctgc tcgcgcggcg tgaagctgct ggcggatgcg ccgctggtcg aggtggctga
    27181 tggcgaatat gacgtgatcg tactgcctgg tggcattaaa ggcgcagagt gttttcgcga
    27241 tagtaccctg ctggttgaaa ccgttaaaca gttccaccgt tccgggcgta tcgtcgcggc
    27301 tatttgcgcc gcgccagcca ccgtgctggt gccgcacgat atcttcccga ttggtaatat
    27361 gaccggcttc ccgacgctga aagacaaaat tcccgctgaa caatggcagg acaagcgcgt
    27421 cgtctgggat gcacgggtaa aattgctgac cagccagggg ccggggacag ctatcgactt
    27481 tggtctgaaa attatcgacc tgctggttgg gcgcgagaaa gcccatgaag tggcatcaca
    27541 actggtgatg gcggcaggga tttataatta ttacgagtag tgtcggatgc ggtaagcgtc
    27601 gcatctgacc agatgcgacg ttacaaccac aaattacggg cgatatacct tcacattgtt
    27661 aaagccctgc tcgcgcagat agagcgcctg cagacggctc atcaccccgc gctcacacca
    27721 cagtagccag gttctgttct ggtcgagatc gccaaacttg gtgctcagtt tatagaacgg
    27781 cagagaaact acatcaatcc cttcgacttt cagtggctta tcttcctgtt catcgataga
    27841 acggatatcg aggatcacgt cgttcgggcc gaagccattg acggtttcca cttccaccac
    27901 ttcctgctcg gtctgctggg cgatttcgcg gatatcaacg ttattcgctt cttcaaccac
    27961 tttatcgaga atgctgaagt cgaatttctc ttcttccgct tcaatcttcg acttaaccgc
    28021 tttcaccgtc gggcttttgg agataacacc gcaatattcc ggcatcgtgc gagcaaagtc
    28081 ttcggtgcca atctggcgag ccaggttgat gatgtgctct ttgtcgtaag aaatcagcgg
    28141 acgcaggatc agcgtatcgg agacgttatc aatcaggcgc aggttggtca gcgtctgact
    28201 ggacacctgg ccgagcgctt cgccggtgac cagcgcctgt acgccgtaac gttcagccac
    28261 tttagacgcg gcacgcacca tcatacgttt gaggataacg cccatctgac cgtcgtcgat
    28321 tttctcgaga atttccccga cgaccggttc gaaattaata gcgacaaaac gcacgcggtg
    28381 ggagctgcca aaacggttcc acagataatg cgccacctga cgcacgccaa tttcatgcgc
    28441 cgcgccgcca aggttaaaga aacagtaatg cacgcggcag ccgcgacgca tcaacatata
    28501 actggaaaca ccggagtcga aaccaccgga aatgagcgac agcacatctt cctgggtgcc
    28561 gatcgggaaa ccgccaatac cttcgtagcg gcctttaatc agcaggagac gatcgtcttc
    28621 cacttccaga tggacagtca cttccggatt ggtcagcttc acacgcgcgg actcaatatg
    28681 ctgatttaaa ccgccgccga cgtaacgttc cacatcaatc gagctaaaat catgtttgcc
    28741 acggcgcttc acgcgtacgc agaaggtttt gccttccagc tgatcgcgat actgaaccaa
    28801 cgctttctca aaaatatcgt gcatgtcggt aaacggcacg tcttcgactt cgagaatatg
    28861 gtggataccc ggaatacggg tcagggcgtc gcgaatagcc agacgctggt tttcatcttt
    28921 tgcgcgaact tcgatgttat cccagtggcg gacgacagcg agcgtctcat catagtgctt
    28981 taaaacgtta cgaatgttcc cggtaaggat ttttataaag cgcaagcgca cagattggct
    29041 tttgatggtg atttccggga acaatttaat gataaacttc atggcggcaa tggttcgttg
    29101 gcaagcctta agcgacttgt atagggaaaa atacagcagc ccacacctgc ggctgcatcc
    29161 aggcgcggaa gtataccact aacatcgctt tgctgcgcac atcaccttac cattgcgcgt
    29221 tatttgctat ttgccctgag tccgttacca tgacggggcg aaaaatattg agagtcagac
    29281 attcattatg ccgaagaaaa atgaggctcc cgccagcttt gaaaaggcgc tgagcgagct
    29341 ggaacagatt gtaacccgtc tggaaagtgg cgacctgccg ctggaagagg cgctgaacga
    29401 gttcgaacgc ggcgtgcagc tggcacgtca ggggcaggcc aaattacaac aagccgaaca
    29461 gcgcgtacaa attctgctgt ctgacaatga agacgcctct ctaacccctt ttacaccgga
    29521 caatgagtaa tggactttcc gcagcaactc gaagcctgcg ttaagcaggc caaccaggcg
    29581 ctgagccgtt ttatcgcccc actgcccttt cagaacactc ccgtggtcga aaccatgcag
    29641 tatggcgcat tattaggtgg taagcgcctg cgaccttttc tggtttatgc caccggtcat
    29701 atgttcggcg ttagcacaaa cacgctggac gcacccgctg ccgccgtaga gtgtatccac
    29761 gcttactcat taattcatga tgatttaccg gcgatggatg atgacgatct gcgtcgcggt
    29821 ttgccgacct gccatgtgaa gtttggcgaa gcaaacgcga ttctcgctgg cgacgcttta
    29881 caaacgctgg cgttctcgat tctaagcgat gctgatatgc cggaagtgtc ggatcgcgac
    29941 agaatttcga tgatttctga actggcgagc gccagcggta ttgccggaat gtgcggtggt
    30001 caggcattag atttagacgc ggaaggcaaa cacgtacctc tggacgcgct tgagcgtatt
    30061 catcgtcata aaaccggcgc attgattcgc gccgccgttc gccttggtgc attaagcgcc
    30121 ggagataaag ggcgtcgtgc tctgccagta ctcgacaagt acgcagagag catcggcctt
    30181 gccttccagg ttcaggatga catcctggat gtggtaggag atactgcaac gttgggaaaa
    30241 cgccagggtg ccgaccagca acttggtaaa agtacctacc ctgcacttct gggtcttgag
    30301 caagcccgga agaaagcccg ggatctgatc gacgatgccc gccagtcgct gaaacaactg
    30361 gctgaacaat cactcgatac ctcggcactg gaagcgctag cggactacat catccagcgt
    30421 aataaataaa caataagtat taataggccc ctgatgagtt ttgatattgc caaatacccg
    30481 accctggcac tggtcgactc cacccaggag ttacgactgt tgccgaaaga gagtttaccg
    30541 aaactctgcg acgaactgcg ccgctattta ctcgacagcg tgagccgttc cagcgggcac
    30601 ttcgcctccg ggctgggcac ggtcgaactg accgtggcgc tgcactatgt ctacaacacc
    30661 ccgtttgacc aattgatttg ggatgtgggg catcaggctt atccgcataa aattttgacc
    30721 ggacgccgcg acaaaatcgg caccatccgt cagaaaggcg gcctgcaccc gttcccgtgg
    30781 cgcggcgaaa gcgaatatga cgtattaagc gtcgggcatt catcaacctc catcagtgcc
    30841 ggaattggta ttgcggttgc tgccgagaaa gaaggcaaaa atcgccgcac cgtctgtgtc
    30901 attggcgatg gcgcgattac cgctggcatg gcgtttgaag cgatgaatca cgcgggcgat
    30961 atccgtcctg atatgctggt ggtcctcaac gacaatgaaa tgtcgatttc cgaaaatgtc
    31021 ggcgcgctca ataaccatct ggcacagctg ctttccggta agctttactc ttcgctgcgc
    31081 gaaggcggga aaaaagtttt ctctggcgtt ccgccaatta aagagctgct caaacgtacc
    31141 gaagaacata ttaaaggcat ggtagtgcct ggcacgttgt ttgaagagct gggctttaac
    31201 tacatcggcc cggttgacgg tcacgatgtg ctggggctta tcaccacgct gaagaacatg
    31261 cgcgacctga aaggcccgca gttcctgcat atcatgacca aaaaaggtcg tggttatgaa
    31321 ccggcagaaa aagaccccat cactttccac gccgtgccta aatttgatcc ctccagcggt
    31381 tgtttgccga aaagtagcgg cggtttgccg agctattcaa aaatctttgg cgactggttg
    31441 tgcgaaacgg cagcgaaaga caacaagctg atggcgatta ctccggcgat gcgtgaaggt
    31501 tccggcatgg tcgagttttc acgtaaattc ccggatcgtt acttcgacgt ggcaatcgcc
    31561 gagcaacacg cggtgacctt tgccgccggt ctggcgattg gtgggtacaa acccattgtc
    31621 gcgatttact ccactttcct gcaacgcgcc tatgatcagg tgctgcatga cgtggcgatt
    31681 caaaagctcc cggtcctgtt cgccatcgac cgcgcgggca ttgttggtgc tgacggtcaa
    31741 acccatcagg gcgcttttga cctctcttac ctgcgctgca taccggaaat ggtcattatg
    31801 accccgagcg atgaaaacga atgtcgccag atgctctata ccggctatca ctataacgat
    31861 ggcccgtcag cgttgcgcta cccgcgtggc aacgcggtcg gcgtggaact gacgccgctg
    31921 gaaaaactac caattggcaa aggcattgtg aagcgtcgtg gcgagaaact ggcgatcctt
    31981 aactttggta cgctgatgcc agaagcggcg aaagtcgccg aatcgctgaa cgccacgctg
    32041 gtcgatatgc gttttgtgaa accgcttgat gaagcgttaa ttctggaaat ggccgccagc
    32101 catgaagcgc tggtcaccgt agaagaaaac gccattatgg gcggcgcagg cagcggcgtg
    32161 aacgaagtgc taatggccca tcgtaaacca gtacccgtgc tgaacattgg cctgcctgac
    32221 ttctttattc cgcaaggaac tcaggaagaa atgcgcgccg aactcggcct cgatgccgcc
    32281 ggtatggaag ccaaaatcaa ggcctggctg gcataatccc tactccactc ctgctatgct
    32341 taacaaatta ttcatagact ctaaataatt cgagttgcag gaaggcggca aacgagtgaa
    32401 gccccaggag cttacataag taagtgactg gggtgaacga atgcagccgc agcacatgca
    32461 acttgaagta tgacgagtat ggcaggagtg gcagcatgca atacaacccc ttaggaaaaa
    32521 ccgaccttcg cgtttcccga ctttgcctcg gctgtatgac ctttggcgag ccagatcgtg
    32581 gtaatcacgc atggacactg ccggaagaga gtagccgtcc catcattaaa cgcgcgctgg
    32641 aaggcggcat aaatttcttt gataccgcca atagctattc cgatggcagc agcgaagaga
    32701 tcgttggtcg cgcactgcgg gatttcgccc gtcgtgaaga cgtggtcgtt gcgacgaaag
    32761 tgttccatcg cgttggtgat ttaccggaag gattatcccg tgcacaaatt ttgcgctcta
    32821 tcgacgacag cctgcgccgt ctcggcatgg attatgtcga tatcctgcaa attcatcgct
    32881 gggattacaa cacgccgatc gaagagacgc tggaagccct gaacgacgtg gtaaaagccg
    32941 ggaaagcgcg ttatatcggc gcgtcatcca tgcacgcttc gcagtttgct caggccctga
    33001 agctacaaaa acagcacggc tgggcgcagt ttatcagtat gcaggatcac tacaatctga
    33061 tttatcgcga agaagagcgc gagatgctgc ctctgtgtta tcaggagggc gtggcggtga
    33121 ttccgtggag cccgctggcg cgggggcgac tgacgcgtcc gtggggagaa actaccgcac
    33181 gactggtgtc tgatgaggtg gggagaaatc tctataaaga aagcgatgaa aatgacgcgc
    33241 agatcgcaga gcggttaacg ggcgtcagtg aagaactcgg tgcaacacga gcacaagttg
    33301 cgctggcctg gttgttgagt aaaccgggca ttgccgcacc gattatcggt acatcgcggg
    33361 aagaacagct tgatgagctg ctgaacgcgg tggatatcac tttgaagcca gaacagattg
    33421 ccgaactgga aacgccgtat aaaccgcatg cggtcgtagg atttaaataa gagtccaggc
    33481 ctgatgagac gtgacaagcg tcacatcagg catcggtgca caactacgac agaataccca
    33541 gcggccagtg atgaccgata aagtacagga tgcctgcgga aatcaccccg gcgacaatat
    33601 cgtcgatcat gatccccatg ccgccatgta cattgcgatc aaaccagcgg atcggccacg
    33661 gcttccacat atccagaata cggaaaatca caaacccggc ggcaacccac tgccagtcat
    33721 tggtcggcag cgccatgagc gtgatccaca taccaataaa ttcgtcccag acaatgctgc
    33781 cgtgatcgtg cacgcccata tctttcgccg tttgatgaca aagatagacg ccgatacaga
    33841 tccccaacat caccaccagc gagtagagct gccagggtaa aaaggtcatc agataccaga
    33901 acggaatcgc tgccagcgat cccatcgtgc caggaacgat tgggcttaat ccacttccga
    33961 atccgacagc aagcagatgc cacggattac tcatcttcag gcgacttttc gcgacatctt
    34021 tatggcgtgg caaaatggtc atatcctttc cagtcaaacg tgacaggttc gccgtcacga
    34081 ataaaacaaa gcccttcgat atcggcggtc atttgcccga tacaggtaaa cggtacgccc
    34141 aggtgtccga gagccacatc cagcgcgcca cggttcagtt ccggcacagt gaaacacaac
    34201 tcgtaatctt caccgccaga gagcgcccag cgcagcgcct gttccggttc aacatggcga
    34261 gaaagcgcat cagaaaacgg cagcaatgcc aggtcaatac gtgcgccgca gtcgctggct
    34321 ttcacgatat gcccgagatc ggaaatcaga ccgtcagaga gatcgatagc tgaatttgcc
    34381 agatcgcgca gtgcctgtcc ctgtaaaata cgcggcgatg gacggagatg acgtttgatc
    34441 aagtagtccg catctttagc atcggcaacc tgcaaacggt tttgcaaaat cgccagcccg
    34501 gcggcgctat cgcccggtgt accggtcaca tagatccagt cacccggttt cgctccagag
    34561 cgcgttaagg ctcgtcccat cggaacaaag ccgtggatac ccaacgtcat tgataatggc
    34621 ccacgcgtgg tatcgccgcc aatgagttgc atatcgtaat aattgagaag atcaaacaaa
    34681 ctgtcgctga aggactcaag ccacgcttcg tctacgtccg gtaaggttaa tgccagcgtc
    34741 agccaggccg gatcggcccc catcgctgcc agatcgctta ggttcaccgc cagtgcttta
    34801 tacgccagat cagcaggatc gatatcatgg aggaagtggt tgcccgccac cagcgtatca
    34861 gtgctgatcg ccagggtctg tttctcgggg atattgagaa gagcgcaatc gtcgccgatg
    34921 cccagttcga catcaagacg agaacttctt acacggtcaa aataacgggc aatcagggag
    34981 aactcgccac atgccatacg ttatgcctca gcaggtaaaa aagaaaaggc cggcgatcgc
    35041 ggaacggtct tccgtgaatc taccggcctg gatatcactt tttgttaggg cgaatcacag
    35101 gtgctgcttt atcgagtacg ccgttgacga acttatggct gtcttctgcg ccgaacgatt
    35161 tcgccagttc gatcgcttcg ttaatggcca ctttgtatgg cacatcgcta cgtttagaca
    35221 gctcgtacag cgcaatgcgc agtactgctt tttctacctg tcccagttct tccagcaggc
    35281 gggacaggta tggcttcatc agtccgtcga ggtatgcggt gttagtcgcc accccggcca
    35341 gcagctcacg gaagtacagg acgtcaacgt cttttacatc ctgttcagcc aggaactggt
    35401 attcaacatc agcgatgtcg ttctgggaca actgccagga gtagagcgcc tggacggcac
    35461 actcacgagc gcggcgacga gcagcaggtt tcacggattt ccccttacta atttcaggcc
    35521 ttgatggctt tcaatacatt aatcatttca agcgcggtca gtgcagcttc tgcacctttg
    35581 ttgccagctt tggtgccagc acgttcgatc gcttgttcaa tgctttcagt ggtcagaacc
    35641 ccaaaagcaa ccggaatttc gctgtcctgg gcaacatgcg ccaggccgtt gcttgcacca
    35701 ccagcgacat attcaaagtg ggcagtgcca ccacgaataa ccgtacccag cgcaatcacc
    35761 gcgtcgtatt taccggtttt agccagtgca cccgccgcca gcggcagctc ataggcacca
    35821 ggcacccaaa caacggtaat gttttcatct tttacctgac cgatacgttt cagtgcgtca
    35881 attgcacctt ccagcaggct gtcattgata aagttgttga aacgcgcgat ggtgatggcg
    35941 acgcgagcgt ccggggtagc aacgttagct tcaataatgt tcattttctt ccttcgggtt
    36001 cgagtatggc cccgcagggg ggcggatttt agcataatat ttcgtgcgct gcttcccttt
    36061 cgagccggga gatcatgcac ccactaaatg caggcaaaca tccgggccta catgacgtat
    36121 ctctttgaat ttaaattggg gggcgtcggc taatttctca agccctggca gcgtgcataa
    36181 tccgcgggcg tcgctgccta atagtttagg tgcgatatag acaatcagct catcgactaa
    36241 acccgcctgc agcaatgcgc cagcgagcgt tggccccgct tccacccaga tgctgttaat
    36301 ttgctgttta cccagttgca tcatcagtac aaccagatcc agatgacctt tatgctctgg
    36361 aatcagcaag gtacgcaccg tttccggcca ctcactagaa tcttcctgcg tacgcgcgaa
    36421 ccaggtttcg ccgggctgct gcacaatgcg atgttccggc gtcacgcgat tttggctatc
    36481 aatcacaata cgtactggct ggcggagatt ttgttgcggg tagagcgcct gagtttgttc
    36541 atccagttca gaccaacgca ctgttaaggc tggatcatcc gccagcaccg tggcgctgct
    36601 ggttaaaatg gcatgacttt gtgcgcgctg tcgttgtacg tcgcgccgcg cctgaggcga
    36661 agtgatccac tggctttcgc cgctcgccat cgccgtgcgg ccatcaagcg atgcgccaag
    36721 ttttaactga atataaggaa agccggtgcg catccgcttg agaaagcctt tattcaattg
    36781 ctcggcttca ctcatcatca gcccgtggct gacgtcaatg ccagcctgtt gcagacggta
    36841 aagtccacgc ccagcgacct gcgggttagg atcttgcatc gaggcaacca cgcgcgctac
    36901 gccagcggcg atgagtgcgt cacagcacgg tggcgtacga ccatgatggc tacagggttc
    36961 gagtgtcaca tacgccgttg cgcctttggc tttttcaccc gccatacgca acgcgtgtac
    37021 ttcggcatgt ggttcacccg ctcgttggtg gtaaccttca ccgacaattt cgccatcttt
    37081 gacaatgacg cacccgacat tcgggttggg gtgggtggta aaacgtcctc gttgcgccag
    37141 ctttagcgcc cgcgccatgt aatactcgtc ctgcacggct tagtcctcca ggcgcgcgat
    37201 ctcttcgcca aattctttga tatcttcgaa actgcggtag acagaggcaa aacggatata
    37261 ggcgacttta tcgagctttt tcaattgctc catcaccaga ttgccaatca tcttgctcgg
    37321 cacttcgcgc tcaccggtgg cgcgcagctg cgatttaata tgattgattg ccatttcgac
    37381 gtcatcggaa ctcaccggac gtttttccag cgcccgcagc attccgctac gcaatttctc
    37441 ttcattaaac ggttcacgca cgtcgttgct ttttacaaca cgcggcataa ccagctccgc
    37501 cacttcaaag gtggtgaaac gttcattaca caccagacac tgccgacggc ggcgtacgga
    37561 tgaaccctcg cccacgagac gagagtcaat taccttagtg tccacggcga aacagaatgg
    37621 gcaatgcata cggcgtcctg accaggtggt taacagaaat ctattttacc ctgaactgac
    37681 gatacaacaa aggcgcaacg cttttgagat aaaggatcga tgctttcgct gcttttaccc
    37741 tctaccatta ggctaattgc gacgagaaag ggacgaccta tgaatactaa cgtttttcga
    37801 ctgctcctgc tgggaagcct gttcagcctt agcgcctgtg tgcagcagag cgaagtgcga
    37861 cagatgaaac acagcgtcag cacgctgaac caggagatga cgcaactcaa taaagaaacg
    37921 gtcaaaatca ctcagcaaaa caggctgaat gcaaaatcca gcagtggggt ttaccttctg
    37981 ccaggagcga aaacaccggc aagactggaa agccagatcg gtactttacg tatgtcgctg
    38041 gtgaatatta cgcctgatgc agatggcacc accctgacgc tacgtattca gggcgagtcg
    38101 aatgatccct tgcccgcatt cagcggaact gttgaatatg ggcaaattca gggaacaata
    38161 gacaactttc aggaaatcaa tgtgcagaat caattgatta atgcccctgc cagcgtcctc
    38221 gcccccagcg atgttgatat tccgttacag ttaaagggta tttctgtaga acagttagac
    38281 ttcgtacgta tccacgatat tcaacctgtt atgcagtaaa cgtatttcgg ggcgattttg
    38341 tgcgtcccgc aacatctttc cccgtcattt tgttactccg cttacatcac ccagattgat
    38401 agtaaaagtt tgcaacaagg gtgaaagtca gtacaatccc tgcccgaatg tgtgtaaacg
    38461 tgaacgcaat cgattacgta aatgatagaa ctgtgaaacg aaacatattt ttgtgagcaa
    38521 tgatttttat aataggctcc tctgtatacg aaatatttag aaacgcaatt tgcgcctttt
    38581 tcactcccgc aagggatttt caaacagtgg catacatatg aaaaaaacat tactggcagc
    38641 cggtgcggta ctggcgctct cttcgtcttt tactgtcaac gcagctgaaa acgacaaacc
    38701 gcagtatctt tccgactggt ggcaccagag cgttaacgtt gtcggaagct atcacacccg
    38761 ttttggaccg cagatccgca acgataccta ccttgagtac gaagcattcg ctaaaaaaga
    38821 ctggttcgac ttctatggtt atgcggatgc gccggtattc ttcggcggta actccgatgc
    38881 caaaggtatc tggaaccacg gctctccgct gtttatggaa atcgaaccac gtttctccat
    38941 cgacaagctg accaatactg accttagctt cggtccgttc aaagagtggt acttcgcgaa
    39001 caactacatt tacgacatgg gtcgtaataa agatggtcgc cagagcacct ggtatatggg
    39061 tctgggtacc gacatcgata ctggtctgcc gatgagcctg tccatgaacg tctatgcgaa
    39121 ataccagtgg cagaactatg gcgcagcgaa cgaaaacgag tgggacggtt accgtttcaa
    39181 aattaaatac tttgtgccga ttaccgatct gtggggcggt cagctgagct acatcggctt
    39241 caccaacttc gactggggtt ccgatttagg ggatgacagc ggtaacgcaa tcaacggtat
    39301 taagacccgt actaataact ctatcgcttc cagccatatt ctggctctga actacgatca
    39361 ctggcactac tctgtcgtag ctcgttactg gcacgacggt ggtcagtgga acgacgatgc
    39421 agaactgaac ttcggcaacg gcaacttcaa cgttcgctct accggctggg gtggttacct
    39481 agttgtaggt tacaacttct gattatgaaa atgccgggat ttattcccgg catttcagat
    39541 tgttaacaac gtgcgctttg ttcatgccgg atgcggcgta aacgctttgt ccgacccaca
    39601 aaagcacgta aattcaatat attgctgaaa taacgtagtc ctgataagcg tagcgcatca
    39661 ggcaatttta cgttttaatc acttcttctt gttcatcatc gctttcaggt cagcgaatgg
    39721 gttgtacttc gcttcaccga catctttctg cgcatcttcc cctgcgataa cggtcgtacc
    39781 atactgatcc gcttcggtat atttcgaatg ctcatgatcg tggcaataga gacaaagcaa
    39841 ttcccagtta ctgccatctt ccgggttatt ggtatggtcg tgatcaatgt ggtgaacagt
    39901 aagttcacgc aggttggaat agacaaactc gcgggaacag cgaccgcaga cccacggata
    39961 gatttttaat gctttttcgc gatagccgct ttctaaccgc gcatagtttt ttgggatgat
    40021 agccatgaaa agttacctgg atgaaatgta gggttacgcg cagtttccca agatgcgggg
    40081 attttcgcaa tctccataca tcaaaaaaaa tcccgatctt ctgaccggga ttttcaacat
    40141 caacgggaac ttgattaagg cagaattgac ggctgatccg ccccttcttt ttccactttc
    40201 tgctgcaaca tgtgttcgcg cttcataccc agtttcagag ccaacgcaga tgccacatag
    40261 atggaagatg cagtaccgat ggaaacaccg ataagcatgg tcagcgagaa gccttccagt
    40321 accggaccac cgaagaggta cagcatcagg ataaccatca aggtagtacc ggatgtgatc
    40381 aaggtacggt gcagcgtctg ggtcaaggac acgttaaaga tttcgtaagg cgtaccgcga
    40441 cggatcttgc ggaagttttc acgaatacgg tccgatacca cgatactgtc gttaagcgag
    40501 taaccgataa ccgacatcaa cgatgccaca atggtcaggt caatctcgat atggaataac
    40561 gacaaaatac ccagcgtaat gataacgtcg tgcgccagcg caataaccac ccctgccgcc
    40621 agtcgccact caaagcggaa acctacgtac acgaggatag acagcagcgc tgccatcaac
    40681 gccatcgcac cggtttgcgc aaggtctgcc cccacgctcg gaccgacgaa ctcaatacgc
    40741 ttcactgctg cattctgatt ggtggattcg ttaatcacct tcagaacctg gctgcccagc
    40801 acctgaccgc cggtttcgcc ttcagcaggc ggcatacgga ccatgatgtc gtggctgcta
    40861 ccaaagtttt gcagcattgg ctcttcaaaa ccggcttttt gcaatgcatc acgcattacg
    40921 tcaatttcag ccggtttttc gagcgtaatt tcgataaccg taccaccggt aaaatccagc
    40981 ccccagttaa agccgcgcac gcccataata acgatagcag cgattaacag cagaccagag
    41041 atgccgaaag cccagtagtc ccagcgcata aagtcataga ctttacggcc gtggtttagt
    41101 tgttcaacag tatattcctg tgccacatcg cactcctcag attgacagct tcttgacgcg
    41161 cttgccgcca tatagcaggt ttacgatggc acgcgtaccg acaatcgcgg taaacatcga
    41221 cgtcgccaca ccgataccgg tagtaatcgc gaacccttta attgccccgg tacccactgc
    41281 gtacaggatg atgactttaa tcagcgtggt gatgttcgca tcgaagatag aactgaatgc
    41341 gccacgataa ccttcatcaa ttgcctgttg aacagtacgt ccgttgctca actcttcttt
    41401 aatacgttcg ttgatcagta cgttcgcatc gaccgccacc gcaagggtta agacgatacc
    41461 cgcgatacct ggcatactca gcgttgcgcc tggcaacagc gacataatgc cgacgattaa
    41521 gatcaagttg gcaatcagag cactggtcgc aatcagacca aacttcttat agaagatgat
    41581 catgaacaga atagaaacca gcagaccggc aaggcaagcc tccagcccct gttcaatgtt
    41641 ctgcataccc agggttgggc caatggtacg ttcttcaaca atctgaatcg gcgcgatcaa
    41701 cgcaccggca cgcagcagca gtgacagctg acgggcttcg ttcgggttgt tgatgccggt
    41761 gatacggaag ctgttaccca gacgagactg gatgttggcg atgttaatca cctcttcctg
    41821 tttcaccaga accgcacgac catttgcatc tttcttaccg ctgtctttgt actccacaaa
    41881 cagggttgcc atcggtttgc cgatgttgtc cttagtgaag ttagacatga tgttaccacc
    41941 agcgctatcg agcgagatgt taacctgcgg ttggttgtat tcgtcctggc tggaagtgga
    42001 gtcggtgata tggtcaccgg tcagaattac gcgtttgtac agcacaactg gctgaccttc
    42061 gcgggtctgt ttcacttcag agtcgcccgg tacgcgaccg gatgccgcag cggcctggtc
    42121 aacgttggtg tttaccagac ggaattccag cgttgccgtc gcacccagaa tctctttcgc
    42181 acgcgcagtg tcctgaatac ctggcagttc aacaacgata cggtcagcac cctgacgctg
    42241 aaccaccggc tccgccacgc caagttggtt tacacggtta cgcaggatat taatgttctg
    42301 ctgcaccgca tattcacgcg cttcactcag acgagcatcg ctcattaccg cacgcagctg
    42361 gttgctgccc tggctgctaa tcaccaggtc cggatggcgc ttgctcagat acgcaatggc
    42421 ttcatcacga gctttagcat cgcggaaagt aatgctcagg ccgtagttgt tttctttacg
    42481 aacggtggta tacgggatgc ccttttcacg cagatcgctg cgcaggctat cgatattttg
    42541 ttcctggagt ttgccaagcg cggtgtccat atccacttcc atcaggaagt gaacgccgcc
    42601 acgcaggtca aggccgagct tcatcggctc agcgtgaata gctgccagcc agcgcggcgt
    42661 tgccggggca aggttaagcg ccacgacgta tttgtcaccc ataacgccca ttaatgcttc
    42721 acgagcgcgc agctgggtgt cagtggagtc gaagcgcgca agaatagcgc cctcttccag
    42781 tgccacagac ttagcagtta ttttttcttc ttgtaacgtt ttttggacct ggatcagcgt
    42841 ttgctcactg gcggcgactc cgcgcgcacc agtgatctga acagccggat cctcaccaaa
    42901 caggttggga agcgcataca gcagaccgat gacaatcacc acgatcagca tgacgtactt
    42961 ccacaaagga taacggttta acacggcaat tcccttaggg aaaaatttta attacagcgc
    43021 cttcatggtg cctttcggca ggacggcagc tacgaagtca cgtttaataa ctacttcagt
    43081 ggtgtcattc agcgcgatag caatgtagcc gttttccgct actttggtta cgcggccaac
    43141 caggccaccg ttcgtcagaa cttcatcacc tttggcaatg gagtccatca gctttttgtg
    43201 ttctttggtg cgcttctgct gtggacgcag gatcatgaaa tagaaaatca gaccgaatac
    43261 caccagcatc aaaatcaaag acatcgggct accttgcgcc ggtgcacccg ttgccgctac
    43321 cgcatcagaa ataaaaaagc tcattaaatt tccctcatta ttaatattaa tcaacgttca
    43381 aaggtggtac ttctcgcccc tgacgctggt aaaaatcagt tacgaagctc tctaatttac
    43441 cctcttcaat agccttgcgt aaacccgcca tcaaacgctg gtagtaacga aggttatgaa
    43501 tggtgttgag tcgcgcgcct aatatttcgt tgcaacggtc aagatgatgc aagtaagcgc
    43561 gtgaataatt gcgacaggtg tagcaatcac actcaggatc gagtgggcca gtatcgctct
    43621 tatacttcgc attgcggatt ttcaccacgc catcggtcac gaacaaatga ccatttcggg
    43681 cgttgcgggt tggcattacg cagtcaaaca tatcgatacc acgacgtacg ccttcaacca
    43741 ggtcttctgg tttaccaacg cccatcaggt aacgcggttt gtctgccgga atttgcgggc
    43801 atacatgctc cagaatgcgg tgcatatctg ctttcggctc acccacagcc agaccgccga
    43861 cagcgtagcc atcaaaaccg atatctacca gacctttaac agaaatatca cgtaaatctt
    43921 cgtaaacgct gccctgaatg ataccaaaca gcgcattttt gtttccgaga ctgtcaaaac
    43981 gctcacggct acgcttcgcc caacgcagag acatctccat ggagcgtttt gcgtaatccc
    44041 agtcagcagg atacggcgta cactcatcaa agatcatgac gatatccgaa ccaagatcgt
    44101 actgaatctc cattgatttt tcaggatcga ggaaaatcgg atcgccgttg atcgggttac
    44161 ggaagtgcac gccctgttcg gtgattttac gaatatcgcc aaggctgaag acctggaaac
    44221 cgccggagtc ggtgaggatc ggccccttcc actgcataaa atcgtgcaga tcgccgtgca
    44281 gtttcatgat ttcctggccc gggcgcagcc acaggtggaa ggtgttgccg aggataattt
    44341 gcgcgccagt ggcttcaact tcttccggcg tcatcccttt tacggtgccg taggtgccaa
    44401 caggcataaa acaaggcgtt tccactacgc cacgatcaaa gaccaggcgg ccacggcgtg
    44461 cgcgaccgtc ggtggtgtcc agttcaaatt tcattttttc tccactacgt cagaaaaaca
    44521 gtccaacgtt ttaaaccagc gccgcggaat tactccccga cgcgctcatt aattgcctgc
    44581 ggattgtacg tgataaacat cgcatcaccg taactaaaaa agcgatattt ctcttctacc
    44641 gctgctttat aggcgttcat ggtgtgttga taaccggcaa aggccgaaac cagcataatc
    44701 agcgtcgact ctggcaagtg gaagttcgtc accagcgcat cgaccacttt gtactggaag
    44761 cccggataga taaagatttg ggtatcgtcg aagaacggtt caatgagatc gtttttcgct
    44821 gcctgagccg cgctttccag cgaacgtact gaagtggtgc caaccgcaat cacccggtta
    44881 ccgcgcgctt tcgccgccag taccgcgtct accacatcct gcggtacttc agcgtattcc
    44941 gagtgcatga tgtgatcttc aatggtgtcg acgcgcaccg gctggaaggt gcccgcacca
    45001 acgtgcaacg tcacaaacgc catctccacg cctttggcgc gcaatttttc cagcaaaggc
    45061 tcgtcaaaat gcagaccagc ggtcggggct gcaaccgcgc ccggtttttc gctataaacg
    45121 gtttgataaa gttcgcggtc agcgtcttcg tccggacggt cgatatacgg cggcagcggc
    45181 atatggccga tactgttgag gatatccagt accgaacgat catcattgaa ttcgacttca
    45241 aacagtgcgc cgtggcgcgc ggtcattgtt gcgttaatac tttcgtcatc gcccagcagc
    45301 agttctgcgc caggttttgg cgctttcgag gcgcgaatat gcgcaagaat gcgtttgtcg
    45361 tcgagcatcc gctcaaccag cacttcaatc ttgccgccgc tggctttacg cccaaacagg
    45421 cgcgccggga tcacgcgggt attattaaaa accagaagat cgccggggtt gagcttatca
    45481 agtaaatcgg tgaaagtacc gtgcgtcagc gcgcccgtcg gcccgtccag cgacagtaaa
    45541 cgacagctac tgcgttcagg catgggatag tgggcaatca gggattcggg caattcaaag
    45601 gagaaatcgg taacgcgcat gacactgact cagactaaaa taagaggcgg gtagtctagt
    45661 gccggggcgc tctccctgca acctttaccc ccttttggat agaatataat cgatgaattt
    45721 tttagctcac ctgcatttag cccatctcgc ggaaagctcg ctttccggca atttactggc
    45781 tgatttcgta cgcggaaatc ctgaagaaag ttttccgccc gacgtcgtgg ctggcattca
    45841 tatgcatcga cgtatcgacg tattaaccga caatctgccg gaagtccgcg aagcacggga
    45901 atggtttcgt agtgaaactc gccgcgttgc gcctattacg ctggatgtca tgtgggatca
    45961 ctttctttcc cgccactggt cgcagctgtc gccggacttt ccgctacagg aatttgtctg
    46021 ttatgcccgc gagcaagtga tgacgatttt gccggactca ccgccacgtt ttatcaatct
    46081 gaacaattac ttgtggtcag agcagtggct ggtgcgctat cgcgatatgg atttcatcca
    46141 gaacgtgtta aacggcatgg caagccgccg cccacgtctg gatgccctgc gtgactcctg
    46201 gtacgattta gacgctcatt atgacgccct cgaaacccgc ttctggcagt tttatccgcg
    46261 gatgatggcg caggcgtcac acaaggcgtt ataaaacgtt agttcatcca taccgtagcc
    46321 gaaatggcag gcaaagccag aatcccgttt gtcagttgcc catggccttc tttgcgttgc
    46381 cattgcgcga cattaagaaa cggtgacgcg gggagcacca cttcacaggc ctcgccacgg
    46441 ttgattgcca ccagtacacg ttgctgattc agcacgcgga caaataccac tacgttatct
    46501 tccgcataca gcacctgaca gccgccacga cgcagcgcct gacttttctt acgcagcgca
    46561 atcattcgct ggtacagcgc gaataacgcc gtatcctgct tttccacctg ccaggggaac
    46621 ggtttacggc aaaacggatc gtttttgcca tccagtccta cttcatcacc gtaataaatg
    46681 cacggtacac caggccaggt gaacagccag accaccgcca gcggcaggcg cgcaatatcc
    46741 cgaccgagca gcgttttaaa tcgcgcagta tcgtggctgt cgagctgatt aaacatacgt
    46801 aattgttgtt gatgagaaag ccctgcgcgg taattatcca tccaggccat acaggtttgg
    46861 gcatcaattt gctgcggatc gtaagagata tcggtattgg caagaaatcc ccacaacggg
    46921 aatgtgaagc cacgatagtt catggcggca tcttccacat cggcctgtaa ccattgccgt
    46981 gcatcgccaa aatgttcgcc gacaatatac gcttccggct gggtttcttt cgccgcttcg
    47041 gtgatcccag caacgtgctg catat
//
LOCUS       DAZFQD010000019        44784 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.019, whole genome shotgun sequence.
ACCESSION   DAZFQD010000019 DAZFQD010000000
VERSION     DAZFQD010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 44784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44784
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.019"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            263..1357
                     /gene="gcvT"
                     /locus_tag="ACHKTW_001195"
     CDS             263..1357
                     /gene="gcvT"
                     /locus_tag="ACHKTW_001195"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HFO6462257.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQAGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPADRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            1381..1770
                     /gene="gcvH"
                     /locus_tag="ACHKTW_001196"
     CDS             1381..1770
                     /gene="gcvH"
                     /locus_tag="ACHKTW_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005960 - glycine cleavage complex
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HFO6462258.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            1889..4762
                     /gene="gcvP"
                     /locus_tag="ACHKTW_001197"
     CDS             1889..4762
                     /gene="gcvP"
                     /locus_tag="ACHKTW_001197"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HFO6462259.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASDVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPIGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLTRAEAAEINLRSDILNAVGITLDETTTRENVMQLFNVLLGDNHGLDID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWPEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQNELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            5023..5766
                     /gene="ygfF"
                     /locus_tag="ACHKTW_001198"
     CDS             5023..5766
                     /gene="ygfF"
                     /locus_tag="ACHKTW_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO6462260.1"
                     /translation="MVIALVTGGSRGIGRATALLLAQEGYTVAVNYQQNLHAAQEVMN
                     LITQAGGKAFVLQADISDENQVVAMFTAIDQHDEPLAALVNNAGILFTQCTVENLTAE
                     RINRVLSTNVTGYFLCCREAVKRMALKNGGSGGAIVNVSSVASRLGSPGEYVDYAASK
                     GAIDTLTTGLSLEVAAQGIRVNCVRPGFIYTEMHASGGEPGRVDRVKSNIPMQRGGQA
                     EEVAQAIVWLLSDKASYVTGSFIDLAGGK"
     gene            complement(5823..7256)
                     /gene="bglA"
                     /locus_tag="ACHKTW_001199"
     CDS             complement(5823..7256)
                     /gene="bglA"
                     /locus_tag="ACHKTW_001199"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HFO6462261.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGSWNKGGKGPSICDVLTGGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDDMFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKVVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHDNPEETMYQVLHHQFV
                     ASALAVKAARRINPEMKVGCMLAMVPLYPYCCNPDDVMFAQESMRERYVFTDVQLRGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQRPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GAGDMSRSRKKSFNWYQEVIASNGEKL"
     gene            7301..7612
                     /gene="yqfB"
                     /locus_tag="ACHKTW_001200"
     CDS             7301..7612
                     /gene="yqfB"
                     /locus_tag="ACHKTW_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-acetylcytidine aminohydrolase"
                     /protein_id="HFO6462262.1"
                     /translation="MQPNDITFFQRFQDDILAGRKTITIRDESESHFKTGDVLRVGRF
                     EDDGYFCTIEVTATSTVRLDTLTEKHAEQENMTLTELKKVIADIYPGQTQFYVIEFKC
                     L"
     gene            7776..8435
                     /gene="yqfA"
                     /locus_tag="ACHKTW_001201"
     CDS             7776..8435
                     /gene="yqfA"
                     /locus_tag="ACHKTW_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFO6462263.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(8631..9611)
                     /gene="ygfZ"
                     /locus_tag="ACHKTW_001202"
     CDS             complement(8631..9611)
                     /gene="ygfZ"
                     /locus_tag="ACHKTW_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HFO6462264.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMAEDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARAALANLFSELPSKEKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLAGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            9854..10120
                     /gene="sdhE"
                     /locus_tag="ACHKTW_001203"
     CDS             9854..10120
                     /gene="sdhE"
                     /locus_tag="ACHKTW_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HFO6462265.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRNRERGPVAI"
     gene            10101..10508
                     /gene="ygfX"
                     /locus_tag="ACHKTW_001204"
     CDS             10101..10508
                     /gene="ygfX"
                     /locus_tag="ACHKTW_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HFO6462266.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQEWSIGKAPWMIKSGMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(10548..11069)
                     /gene="fldB"
                     /locus_tag="ACHKTW_001205"
     CDS             complement(10548..11069)
                     /gene="fldB"
                     /locus_tag="ACHKTW_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HFO6462267.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIVALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            11181..12077
                     /gene="xerD"
                     /locus_tag="ACHKTW_001206"
     CDS             11181..12077
                     /gene="xerD"
                     /locus_tag="ACHKTW_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFO6462268.1"
                     /translation="MKQDLARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            12102..12812
                     /gene="dsbC"
                     /locus_tag="ACHKTW_001207"
     CDS             12102..12812
                     /gene="dsbC"
                     /locus_tag="ACHKTW_001207"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HFO6462269.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKEMKEFLDEHQKMTSGK"
     gene            12818..14551
                     /gene="recJ"
                     /locus_tag="ACHKTW_001208"
     CDS             12818..14551
                     /gene="recJ"
                     /locus_tag="ACHKTW_001208"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFO6462270.1"
                     /translation="MKQQIQLRRRAVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGETLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLDPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            14642..15740
                     /gene="prfB"
                     /locus_tag="ACHKTW_001209"
     CDS             join(14642..14716,14718..15740)
                     /gene="prfB"
                     /locus_tag="ACHKTW_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFO6462271.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            15750..17267
                     /gene="lysS"
                     /locus_tag="ACHKTW_001210"
     CDS             15750..17267
                     /gene="lysS"
                     /locus_tag="ACHKTW_001210"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFO6462272.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDINPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(17310..17858)
                     /gene="idi"
                     /locus_tag="ACHKTW_001211"
     CDS             complement(17310..17858)
                     /gene="idi"
                     /locus_tag="ACHKTW_001211"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HFO6462273.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTLLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESSEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPSGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLHGIDATPWAFSP
                     WMVMQAANSEARKLLSTFAQHN"
     gene            17913..17984
                     /locus_tag="ACHKTW_001212"
     CDS             17913..17984
                     /locus_tag="ACHKTW_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6462274.1"
                     /translation="MINQVSVYRQPPVLSGCRQVKTI"
     gene            complement(17981..18106)
                     /gene="yqfG"
                     /locus_tag="ACHKTW_001213"
     CDS             complement(17981..18106)
                     /gene="yqfG"
                     /locus_tag="ACHKTW_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqfG"
                     /protein_id="HFO6462275.1"
                     /translation="MNLLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(18108..19556)
                     /gene="uacT"
                     /locus_tag="ACHKTW_001214"
     CDS             complement(18108..19556)
                     /gene="uacT"
                     /locus_tag="ACHKTW_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HFO6462276.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKEAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPLIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIIRGLRVDGVGTMIGGTFNSFPHTSFSQNVGLVSVTRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLKVRTVRMWLLMRKLKKNEHGE"
     gene            19992..21911
                     /gene="ygfT"
                     /locus_tag="ACHKTW_001215"
     CDS             19992..21911
                     /gene="ygfT"
                     /locus_tag="ACHKTW_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     YgfT"
                     /protein_id="HFO6462277.1"
                     /translation="MNKFIAAEAAECIGCHACEIACAVAHNQENWPLSHSDFRPRIHV
                     VGKGQAANPVACHHCNNAPCVTACPVNALTFQSDSVQLDEQKCIGCKRCAIACPFGVV
                     EMVDTIAQKCDLCNQRSSGTQACIEVCPTQALRLMDDKGLQQIKVARQRKTAAGKASS
                     DAQPSRSAALLPVNSRKGADKISASERKTHFGEIYCGLDPQQATYESDRCVYCAEKAN
                     CNWHCPLHNAIPDYIRLVQEGKIIEAAELCHQTSSLPEICGRVCPQDRLCEGACTLKD
                     HSGAVSIGNLERYITDTALAMGWRPDVSKVVPRIEKVAVIGAGPAGLGCADILARAGV
                     QVDVFDRHPEIGGMLTFGIPPFKLDKTVLSQRREIFTAMGIDFHLNCEIGRDITFSDL
                     TSEYDAVFIGVGTYGMMRADLPHEDAPGVIQALPFLTAHTRQLMGLPESEEYPLTDVE
                     GKRVVVLGGGDTTMDCLRTSIRLNAASVTCAYRRDEVSMPGSRKEVVNAREEGVEFQF
                     NVQPQYIACDEDGRLTAVGLIRTAMGEPGPDGRRRPRPVAGSEFELPADVLIMAFGFQ
                     AHAMPWLQGSGIKLDKWGLIQTGDVGYLPTQTHLKKVFAGGDAVHGADLVVTAMAAGR
                     QAARDMLTLFDTKAS"
     gene            21911..22399
                     /gene="ygfS"
                     /locus_tag="ACHKTW_001216"
     CDS             21911..22399
                     /gene="ygfS"
                     /locus_tag="ACHKTW_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFO6462278.1"
                     /translation="MKSLIIVNPADCIGCRTCEVACVVAHPSEQELNADVFLPRLKVQ
                     RLDSISAPVMCHQCENAPCVGACPVGALTMGEQVVQTNSARCIGCQSCVSACPFGMIT
                     IQSLPGDTRQQIVKCDLCEQREEGPACVESCPTQALQLLTERELRRVRQQRIVASGEN
                     PL"
     gene            complement(22435..23802)
                     /gene="ghxQ"
                     /locus_tag="ACHKTW_001217"
                     /gene_synonym="ygfQ"
     CDS             complement(22435..23802)
                     /gene="ghxQ"
                     /locus_tag="ACHKTW_001217"
                     /gene_synonym="ygfQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxQ"
                     /protein_id="HFO6462279.1"
                     /translation="MSGDILQTPDAPKPQGALDNYFKITARGSTVRQEVLAGLTTFLA
                     MVYSVIVVPGMLGKAGFPPAAVFVATCLVAGFGSLLMGLWANLPMAIGCAISLTAFTA
                     FSLVLGQQISVPVALGAVFLMGVIFTAISVTGVRTWILRNLPMGIAHGTGIGIGLFLL
                     LIAANGVGMVIKNPIEGLPVALGAFTSFPVMMSLLGLAVIFGLEKCRVPGGILLVIIA
                     ISIIGLIFDPAVKYHGLVAMPSLTGEDGKSLIFSLDIMGALQPTVLPSVLALVMTAVF
                     DATGTIRAVAGQANLLDKDNQIINGGKALTSDSVSSIFSGLVGAAPAAVYIESAAGTA
                     AGGKTGLTATVVGALFLLILFLSPLSFLIPGYATAPALMYIGLLMLSNVSKLDFNDFI
                     DAMAGLVCAVFIVLTCNIVTGIMLGFVTLVVGRVFAREWQKLNIGTVIITAALVAFYA
                     GGWAI"
     gene            complement(23838..25154)
                     /gene="guaD"
                     /locus_tag="ACHKTW_001218"
     CDS             complement(23838..25154)
                     /gene="guaD"
                     /locus_tag="ACHKTW_001218"
                     /EC_number="3.5.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008892 - guanine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006147 - guanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanine deaminase"
                     /protein_id="HFO6462280.1"
                     /translation="MSGEHTLKAVRGSFIDVTRTVDNPEEIASALRFIEDGLLLIKQG
                     KVEWFGEWEDGKHQIPDTIRVRDYRGKLIVPGFVDTHIHYPQSEMVGAYGEQLLEWLN
                     KHTFPTERRYEDLEYAREMSAFFIKQLLRNGTTTALVFGTVHPQSVDALFEAASHINM
                     RMIAGKVMMDRNAPDYLLDTAESSYHQSKELIERWHKNGRLLYAITPRFAPTSSPEQM
                     AMAQRLKEEYPDTWVHTHLCENKDEIAWVKSLYPDHDGYLDVYHQYGLTGKNCVFAHC
                     VHLEEKEWDRLSETKSSIAFCPTSNLYLGSGLFNLKKAWQKKVKVGMGTDIGAGTTFN
                     MLQTLNEAYKVLQLQGYRLSAYEAFYLATLGGAKSLGLDDLIGNFLPGKEADFVVMEP
                     TATPLQQLRYDNSVSLVDKLFVMMTLGDDRSIYRTYVDGRLVYERN"
     gene            complement(25172..26572)
                     /gene="xanQ"
                     /locus_tag="ACHKTW_001219"
     CDS             complement(25172..26572)
                     /gene="xanQ"
                     /locus_tag="ACHKTW_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanQ"
                     /protein_id="HFO6462281.1"
                     /translation="MSDINNAGSDLIFELEDRPPFHQALVGAITHLLAIFVPMVTPAL
                     IVGAALQLSAETTAYLVSMAMIASGIGTWLQVNRYGIVGSGLLSIQSVNFSFVTVMIA
                     LGSSMKSDGFHEELIMSSLLGVSFVGAFLVVGSSFILPYLRRVITPTVSGIVVLMIGL
                     SLIKVGIIDFGGGFAAKSSGTFGNYEHLGVGLLVLIVVIGFNCCRSPLLRMGGIAIGL
                     CVGYIASLCLGMVDFSSMRNLPLITIPHPFKYGFSFSFHQFLVVGTIYLLSVLEAVGD
                     ITATAMVSRRPIQGEEYQSRLKGGVLADGLVSVIASAVGSLPLTTFAQNNGVIQMTGV
                     ASRYVGRTIAVMLVILGLFPMIGGFFTTIPSAVLGGAMTLMFSMIAIAGIRIIITNGL
                     KRRETLIVATSLGLGLGVSYDPEIFKILPASIYVLVENPICAGGLTAILLNIILPGGY
                     RQENVLPGITSAEEMD"
     gene            complement(26737..29607)
                     /gene="xdhD"
                     /locus_tag="ACHKTW_001220"
     CDS             complement(26737..29607)
                     /gene="xdhD"
                     /locus_tag="ACHKTW_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     Mo/Fe-S-binding subunit"
                     /protein_id="HFO6462282.1"
                     /translation="MIIHFTLNGAPQELTVNPGENVQKLLFNMGMHSVRNSDDGFGFA
                     GSDAIIFNGNIVNASLLIAAQLEKADIRTAESLGKWNELSLVQQAMVDVGVVQSGYND
                     PAAALIITDLLDRIAAPTREEIDDALSGLFSRDAGWQQYYQVIELAVARKNNPQATID
                     IAPTFRDDLEVIGKHYPKTDAAKMVQAKPCYVEDRVTADACVIKMLRSPHAHALITHL
                     DVSKAEALPGVVHVITHLNCPDIYYTPGGQSAPEPSPLDRRMFGKKMRHVGDRVAAVV
                     AESEEIALEALKLIDVEYEVLKPVMSIDEAMAEDAPVVHDEPVVYVAGAPDTLEDDNS
                     HAAQRGEHMIINFPIGSRPRKNIAASIHGHIGDMDKGFADADVIIERTYNSTQAQQCP
                     TETHICFTRMDGDRLVIHASTQVPWHLRRQVARLVGMKQHKVHVIKERVGGGFGSKQD
                     ILLEEVCAWATCVTGRPVLFRYTREEEFIANTSRHVAKVTVKLGAKKDGRLTAVKMDF
                     RANTGPYGNHSLTVPCNGPALSLPLYPCDNVDFQVTTYYSNICPNGAYQGYGAPKGNF
                     AITMALAELAEQLQIDQLEIIERNRVHEGQELKILGAIGEGKAPTSVPSAASCALEEI
                     LRQGREMIQWSSPKPQNGDWHIGRGVAIIMQKSGIPDIDQANCMIKLESDGTFIVHSG
                     GADIGTGLDTVVTKLAAEVLHCPPQDVHVISGDTDHALFDKGAYASSGTCFSGNAARL
                     AAENLREKILFHGAQMLGQAVADVQLATPGVVRGKKGEVSFGDIAHKGETGTGFGSLV
                     GTGSYITPDFAFPYGANFAEVAVNTRTGEIRLDKFYALLDCGTPVNPELALGQIYGAT
                     LRAIGHSMSEEIIYDAEGHPLTRDLRSYGAPKIGDIPRDFRAVLVPSDDKVGPFGAKS
                     ISEIGVNGAAPAIATAIHDACGIWLREWHFTPEKILTALEKI"
     gene            complement(29604..30383)
                     /gene="ygfM"
                     /locus_tag="ACHKTW_001221"
     CDS             complement(29604..30383)
                     /gene="ygfM"
                     /locus_tag="ACHKTW_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     FAD-binding subunit"
                     /protein_id="HFO6462283.1"
                     /translation="MIEQFFRPDSVEQALELKRRYQDEAVWFAGGSKLNATPTRTDKK
                     IAISLQDLELDWVDWDNGALRIGAMSRLQPLRDARFIPAALREALGFVYSRHVRNQST
                     IGGEIAARQEESVLLPVLLALDAELVFGNGETLSIEDYLACPCDRLLTEIIIKDPYRT
                     CATRKISRSQAGLTVVTAAVAMTDHDGMRIALDGVASKALRLHDVEKQNLEGNALEQA
                     VANAIFPQEDLRGSVAYKRYITGVLVADLYADCQQAGEEAV"
     gene            complement(30434..31762)
                     /gene="ssnA"
                     /locus_tag="ACHKTW_001222"
     CDS             complement(30434..31762)
                     /gene="ssnA"
                     /locus_tag="ACHKTW_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HFO6462284.1"
                     /translation="MLILKNVTAVQLHPAKVQEGVDIAIENDVIVAIGDALTQRYPDA
                     SYKEMHGRIVMPGIVCSHNHFYSGLSRGIMANIAPCPDFISTLKNLWWRLDRALDEES
                     LYYSGLICSLEAIKSGCTSVIDHHASPAYIGGSLSTLRDAFLKVGLRAMTCFETTDRN
                     NGIKELQEGVEENIRFARQIDEAKKAATEPYLVEAHIGAHAPFTVPDAGLEMLREAVK
                     ATGRGLHIHAAEDLYDVSYSHHWYGKDLLARLAQFDLIDSKTLVAHGLYLSKDDIALL
                     NQRDAFLVHNARSNMNNHVGYNHHLSDIRNLALGTDGIGSDMFEEMKFAFFKHRDAGG
                     PLWPDSFAKALTNGNELMSRNFGAKFGLLEAGYKADLTICDYNSPTPLLADNIAGHIA
                     FGMGSGSVHSVMVNGVMVYEDRQFNFDCDSIYAQARKAAASMWRRMDALA"
     gene            complement(31765..34863)
                     /gene="ygfK"
                     /locus_tag="ACHKTW_001223"
     CDS             complement(31765..34863)
                     /gene="ygfK"
                     /locus_tag="ACHKTW_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenate reductase subunit YgfK"
                     /protein_id="HFO6462285.1"
                     /translation="MGDIMRPIPFEELLTRIFDEYQQQRSIFGIPEQQFYSPVKGKTV
                     SVFGETCATPVGPAAGPHTQLAQNIVTSWLTGGRFIELKTVQILDRLELEKPCIDAED
                     ECFNTEWSTEFTLLKAWDEYLKAWFALHLLEAMFQPSDSGKSFIFNMSVGYNLEGIKQ
                     PPMQQFIDNMMDASDHPKFAQYRDTLNKLLQDDAFLARHGLQEKRESLQALPARIPTS
                     MVQGVTLSTMHGCPPHEIEAICRYMLEEKGLNTFVKLNPTLLGYARVREILDVCGFGY
                     IGLKEESFDHDLKLTQALEMLERLMALAKEKSLGFGVKLTNTLGTINNKGALPGEEMY
                     MSGRALFPLSINVAAVLSRAFDGKLPISYSGGASQLTIRDIFDTGIRPITMATDLLKP
                     GGYLRLSACMRELEGSDAWGLDHVDVERLNRLAADALTMEYTQKHWKPEERIEVAEDL
                     PLTDCYVAPCVTACAIKQDIPEYIRLLGEHRYADALELIYQRNALPAITGHICDHQCQ
                     YNCTRLDYDSALNIRELKKVALEKGWDEYKQRWHKPAGSGSRHPVAVIGAGPAGLAAG
                     YFLARAGHPVTLFEREANAGGVVKNIIPQFRIPAELIQHDIDFVADHGVKFEYGCSPD
                     LTVEQLKNQGFHYVLIATGTDKNSGVKLAGDNQNVWKSLPFLREYNKGTALKLGKHVV
                     VVGAGNTAMDCARAALRVPGVEKATIVYRRSLQEMPAWREEYEEALHDGVEFRFLNNP
                     ERFDADGTLTLRVMSLGEPDEKGRRRPVETNETVTLHVDSLITAIGEQQDTEALNAMG
                     VPLDKNGWPDVDHNGETRLTDVFMIGDVQRGPSSIVAAVGTARRATDAILSRENIRSH
                     QNDKYWNNVNPAEIYQRKGDISITLVNSDDRDAFVAQEAARCLECNYVCSKCVDVCPN
                     RANVSIAVPGFQNRFQTLHLDAYCNECGNCAQFCPWNGKPYKDKITVFSLAQDFDNSS
                     NPGFLVEDCRVRVRLNNQSWVLNIDSEGQFNNVPPELNDMCRIISHVHQHHHYLLGRV
                     EV"
     gene            complement(35185..35763)
                     /gene="mocA"
                     /locus_tag="ACHKTW_001224"
     CDS             complement(35185..35763)
                     /gene="mocA"
                     /locus_tag="ACHKTW_001224"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HFO6462286.1"
                     /translation="MSAIDCIITAAGLSSRMGQWKMMLPWQQGTILDTSIKNALQFCS
                     RIILVTGYRGNELHERYANQSNITIIHNPDYAQGLLTSVKAAVPAVQTEHCFLTHGDM
                     PTLTIDIFRKIWSLRNDGAILPLHNGIPGHPILVSKPCLMQAIQRPNVTNMRQALLMG
                     AHYSVEIENAEIILDIDTPDDFITAQKRYTKI"
     gene            35866..36636
                     /gene="yqeC"
                     /locus_tag="ACHKTW_001225"
     CDS             35866..36636
                     /gene="yqeC"
                     /locus_tag="ACHKTW_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium cofactor biosynthesis protein YqeC"
                     /protein_id="HFO6462287.1"
                     /translation="MKSIVDPSALVIDLGAQKRPTVISVVGAGGKTSLLFWLAELLQA
                     SGRRVLITTTTHMFMPTSHWPVVFCRDPAMLPHASLTSPISFCFHSWKANQGKVQGFT
                     PEAIDALVQRPECDVILIEADGSRGMPLKAPDEHEPCIPKSSCCVIAVMGGHTLGAKV
                     STENVHRWSQFADITGLTPDATLQLSDLVALVRHPQGAFKNVPQGCRRVWFINRFSQC
                     ENAIAQSELLQPLQQHDVEAIWLGDIQEHPAIARRFVN"
     gene            36684..38309
                     /gene="yqeB"
                     /locus_tag="ACHKTW_001226"
     CDS             36684..38309
                     /gene="yqeB"
                     /locus_tag="ACHKTW_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent molybdenum cofactor
                     biosynthesis protein YqeB"
                     /protein_id="HFO6462288.1"
                     /translation="MNIFTEAAKLEEQNCPFAMAQIVDSRGSTPRHSAQMLVRADGSI
                     VGTIGGGMVERKVIEESLQALQERKPRLFHGRMARNGADAVGSDCGGAMSVFISVHGM
                     RPRLVLIGAGHVNRAIAQSAALLGFDIAVADIYRESLNPELFPPSTTLLHAESFGAAV
                     EALDIRPDNFVLIATNNQDREALDKLIEQPIAWLGLLASRRKVQLFLRQLREKGVAEE
                     HIARLHAPVGYNIGAETPQEIAISVLAEILQVKNNAPGGLMMKPSHPSGHQLVVIRGA
                     GDIASGVALRLYHAGFKVIMLEVEKPTVIRCTVAFAQAVFDGEMTVEGVTARLATSSA
                     EAMKLTERGFIPVMVDPACSLLDELKPLCVVDAILAKQNLGTRADMAPVTIALGPGFT
                     AGKDCHAVIETNRGHWLGQVIYSGCAQENTGVPGNIMGHTTRRVIRAPAAGIMRSNVK
                     LGDLVKEGDVIAWIGEHEIKAPLTGMVRGLLNDGLAVVGGFKIGDIDPRGETADFSSV
                     SDKARAIGGGVLEALMMLMHQGVKATKEVLEVA"
     gene            complement(38350..39282)
                     /gene="arcC"
                     /locus_tag="ACHKTW_001227"
     CDS             complement(38350..39282)
                     /gene="arcC"
                     /locus_tag="ACHKTW_001227"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFO6462289.1"
                     /translation="MSKKIVLALGGNALGDDLAGQMKAVKITSQAIVDLIAQGHEVIV
                     THGNGPQVGMINQAFEAAAKTEAHSPMLPMSVCVALSQGYIGYDLQNALREELLSRGI
                     NKPVATLVTQVEVDANDPAFLNPTKPIGSFFTEQEAEQLTKQGYTLKEDAGRGYRRVV
                     ASPKPVDIIEKETVKALVDAGQVVITVGGGGIPVIREGNHLRGASAVIDKDWASARLA
                     EMIDADMLIILTAVEKVAINFGKENEQWLDRLSLSDAERFIEEGHFAKGSMLPKVEAA
                     ASFARSRAGREALITVLSKAKEGIEGKTGTVICQ"
     gene            complement(39330..40715)
                     /gene="hyuA"
                     /locus_tag="ACHKTW_001228"
     CDS             complement(39330..40715)
                     /gene="hyuA"
                     /locus_tag="ACHKTW_001228"
                     /EC_number="3.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-phenylhydantoinase"
                     /protein_id="HFO6462290.1"
                     /translation="MRVLIKNGTVVNADGQAKQDLLIESGIVRQLGNNISPQLPYEEI
                     DATGCYVFPGGVDVHTHFNIDVGIARSCDDFFTGTRAAACGGTTTIIDHMGFGPNGCR
                     LRHQLEVYRGYAAHKAVIDYSFHGVIQHINHAILDEIPMMVEEGLSSFKLYLTYQYKL
                     NDDEVLQALRRLHESGALTTVHPENDAAIASKRAEFIAAGLTAPRYHALSRPLECEAE
                     AIARMINLAQIAGNAPLYIVHLSNGLGLDYLRLARANHQPVWVETCPQYLLLDERSYD
                     TEDGMKFILSPPLRNVREQDKLWCGISDGAIDVVATDHCTFSMAQRLQISKGDFSRCP
                     NGLPGVENRMQLLFSSGVMTGRITPERFVELTSAMPARLFGLWPQKGLLAPGSDGDVV
                     IIDPRQSQQIQHRHLHDNADYSPWEGFTCQGAIVRTLSRGETIFCDGTFTGKAGRGRF
                     LRRKPFVPPVL"
     gene            complement(40768..41979)
                     /gene="ygeY"
                     /locus_tag="ACHKTW_001229"
     CDS             complement(40768..41979)
                     /gene="ygeY"
                     /locus_tag="ACHKTW_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HFO6462291.1"
                     /translation="MAKNIPFKLILEKAKDYQADMTRFLRDMVAIPSESCDEKRVVHR
                     IKEEMEKVGFDKVEIDPMGNVLGYIGHGPRLVAMDAHIDTVGIGNIKNWDFDPYEGME
                     TDELIGGRGTSDQEGGMASMVYAGKIIKDLGLEDEYTLLVTGTVQEEDCDGLCWQYII
                     EQSGIRPEFVVSTEPTDCQVYRGQRGRMEIRIDVQGVSCHGSAPERGDNAIFKMGPIL
                     GELQELSQRLGYDEFLGKGTLTVSEIFFTSPSRCAVADSCAVSIDRRLTWGETWEGAL
                     DEIRALPAVQKANAVVSMYNYDRPSWTGLVYPTECYFPTWKVEEDHFTVKALVNAYEG
                     LFGKAPVVDKWTFSTNGVSIMGRHGIPVIGFGPGKEPEAHAPNEKTWKSHLVTCAAMY
                     AAIPLSWLATE"
     gene            complement(42037..43233)
                     /gene="dpaL"
                     /locus_tag="ACHKTW_001230"
     CDS             complement(42037..43233)
                     /gene="dpaL"
                     /locus_tag="ACHKTW_001230"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008838 - diaminopropionate ammonia-lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HFO6462292.1"
                     /translation="MSVFSLKIDIADNKFFNGETSPLFSQSQAKLARQFHQKIAGYRP
                     TPLCALDDLANLFGVKKILVKDESKRFGLNAFKMLGGAYAIAQLLCEKYHLDIETLSF
                     EHLKNAIGEKMTFATTTDGNHGRGVAWAAQQLGQNAVIYMPKGSAQERVDAILNLGAE
                     CIVTDMNYDDTVRLTMQHAQQHGWEVVQDTAWEGYTKIPTWIMQGYATLADEAVEQMR
                     EMGVTPTHVLLQAGVGAMAGGVLGYLVDVYSPQNLHSIIVEPDKADCIYRSGVKGDIV
                     NVGGDMATIMAGLACGEPNPLGWEILRNCATQFISCQDSVAALGMRVLGNPYGNDPRI
                     ISGESGAVGLGVLAAVHYHPQRQSLMEKLALNKDAVVLVISTEGDTDVKHYREVVWEG
                     KHAVAP"
     gene            complement(43291..44478)
                     /gene="ygeW"
                     /locus_tag="ACHKTW_001231"
     CDS             complement(43291..44478)
                     /gene="ygeW"
                     /locus_tag="ACHKTW_001231"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HFO6462293.1"
                     /translation="MKTVNELIKDINSLTSHLHEKDFLLTWEQTPDELKQVLDVAAAL
                     KALRAENISTKVFNSGLGISVFRDNSTRTRFSYASALNLLGLAQQDLDEGKSQIAHGE
                     TVRETANMISFCADAIGIRDDMYLGAGNAYMREVGAALDDGYKQGVLPQRPALVNLQC
                     DIDHPTQSMADLAWLREHFGSLENLKGKKIAMTWAYSPSYGKPLSVPQGIIGLMTRFG
                     MDVTLAHPEGYDLIPDVVEVAKNNAKASGGSFRQVTSMEEAFKDADIVYPKSWAPYKV
                     MEQRTELLRANDHEGLKALEKQCLAQNAQHKDWHCTEEMMELTRDGEALYMHCLPADI
                     SGVSCKEGEVTEGVFEKYRIATYKEASWKPYIIAAMILSRKYAKPGALLEQLLKEAQE
                     RVK"
BASE COUNT        10857 a        11253 c        11836 g        10838 t
ORIGIN      
        1 caaattagct ttctaatggc atcatcttta tatagaaact ggtgtgtttt ttggtttttt
       61 attctgtcgc gattttggcg ttttttaacc ataagctaat gtgatgatca attttacctt
      121 atggttaaca gtctgtttcg gtggtaagtt caggcaaaag agaacgattg cgttggggac
      181 cgggagtggc tccgatgctg ggtttcgtgg tgataatttc accatgaaaa gttgtcagcc
      241 ccgcttattc aatgaggaca agatggcaca acagactcct ttgtacgaac aacacacgct
      301 ttgcggcgct cgcatggtgg atttccacgg ctggatgatg ccgctgcatt acggttcgca
      361 aatcgacgaa catcatgcgg tacgtaccga tgccggaatg tttgatgtgt cacatatgac
      421 catcgtcgat ctccgcggca gccgcacccg ggagtttctg cgttatctgc tggcgaacga
      481 tgtggcgaag ctcaccaaaa gcggcaaagc cctttactcg gggatgttga atgcctctgg
      541 cggtgtgata gatgacctca tcgtctacta ctttactgaa gatttcttcc gcctcgttgt
      601 taactccgcc acccgcgaaa aagacctctc ctggattacc caacacgctg aacctttcgg
      661 catcgaaatt accgttcgtg atgacctttc catgattgcc gtgcaagggc cgaatgcgca
      721 ggcaaaagct gccacactgt ttaatgacgc ccagcgtcag gcggtggaag ggatgaaacc
      781 gttctttggc gtgcaggcgg gcgatctgtt tattgccacc actggttata ccggtgaagc
      841 gggctatgaa attgcgctgc ccaatgaaaa agcggccgat ttctggcgtg cgctggtgga
      901 agcgggtgtt aagccatgtg gcttgggcgc gcgtgacacg ctgcgtctgg aagcgggcat
      961 gaatctttat ggtcaggaga tggacgaaac tatttctcct ttagccgcca acatgggctg
     1021 gactatcgcc tgggaaccgg cagatcgtga ctttatcggt cgtgaagccc tggaagtgca
     1081 gcgtgagcat ggtacagaaa aactggttgg tctggtgatg accgaaaaag gcgtgctgcg
     1141 taatgaactg ccggtacgtt ttaccgatgc gcagggcaac cagcatgaag gcattatcac
     1201 cagcgggact ttctccccga cgctgggtta cagcattgcg ctggcacgcg tgccggaagg
     1261 tattggcgaa acggcgattg tgcaaattcg caaccgtgaa atgccggtta aagtgacgaa
     1321 acctgttttt gtgcgtaacg gtaaagccgt cgcgtgattt acttttttgg agattgattg
     1381 atgagcaacg taccagcaga actgaaatac agcaaagaac acgaatggct gcgtaaagaa
     1441 gccgacggca cttacaccgt tggtataacc gaacatgccc aggagctgtt aggcgatatg
     1501 gtgtttgttg acctgccgga agtgggcgca acggttagcg cgggcgatga ctgcgcggtt
     1561 gccgaatcag taaaagcggc gtcagacatt tatgcgccag taagcggtga aatcgtggcg
     1621 gtaaatgacg cactgagcga ttccccggaa ctggtgaaca gcgaaccgta tgcaggtggc
     1681 tggatcttta aaatcaaagc cagcgatgaa agcgaactgg aatcactgct ggatgcgacc
     1741 gcatacgaag cattgttaga agacgagtaa cggctttatt cctcttctgc gggagaggat
     1801 cagggtgagg aaaatttatg cctcaccctc actctcttcg taaggagaga ggttcacaat
     1861 tcactgcacg tttcaggaac catcgctcat gacacagacg ttaagccagc ttgaaaacag
     1921 cggcgctttt attgaacgcc atatcggacc ggacgccgcg caacagcaag aaatgctgaa
     1981 tgccgttggt gcacaatcgt taaacgcgct gaccggccag attgtgccga aagatattca
     2041 gcttgcgacc ccgccgcagg ttggcgcacc ggcgaccgaa tacgccgcac tggcagaact
     2101 caaggctatt gccagtcgca ataaacgctt cacgtcttac atcggcatgg gttacaccgc
     2161 cgtgcagcta ccgccggtta tcctgcgtaa catgctggaa aatccgggct ggtataccgc
     2221 gtacactccg tatcaacctg aagtctccca gggccgcctt gaagcactgc tcaacttcca
     2281 gcaggtaacg ctggatttga ctggactgga tatggcctct gcttctcttc tggacgaggc
     2341 caccgctgcc gccgaagcaa tggcgatggc gaaacgcgtc agcaaactga aaaatgccaa
     2401 ccgcttcttc gtggcttccg atgtgcatcc gcaaacgctg gatgtggtcc gtactcgtgc
     2461 cgaaaccttt ggttttgaag tgattgtcga tgacgcgcaa aaagtgctcg accatcagga
     2521 cgtcttcggc gtgctgttac agcaggttgg cacaaccggt gaaatccacg actacaccgc
     2581 gcttattagc gaactgaaat cacgcaaaat tgtggtcagt gtcgccgccg atattatggc
     2641 gctggtgctg ttaactgcgc cgggtaaaca gggcgcggat attgtttttg gttcggcgca
     2701 acgcttcggc gtgccgatgg gctacggtgg cccacacgcg gccttctttg cggcgaaaga
     2761 tgaatacaaa cgctcaatgc cgggccgtat tatcggtgta tcgaaagatg cagctggcaa
     2821 taccgcgctg cgcatggcga tgcagactcg cgagcaacat attcgccgtg agaaagcgaa
     2881 ctccaacatt tgtacttccc aggtgctgct ggcaaacatc gccagcctgt atgccgttta
     2941 tcacggcccg attggcctga aacgtatcgc taaccgcatt caccgtctga ccgatatcct
     3001 ggcggcgggc ctgcaacaaa aaggtctgaa actgcgccat gcgcactatt tcgacacctt
     3061 gtgtgtggaa gtggccgaca aagcgggcgt actgacgcgt gccgaagcgg ctgaaatcaa
     3121 cctgcgtagc gatattctga acgcggttgg gatcaccctt gatgaaacaa ccacgcgtga
     3181 aaacgtaatg cagcttttca acgtgctgct gggcgataac cacggcctgg acatcgacac
     3241 gctggacaaa gacgtggctc acgacagccg ctctatccag cctgcgatgc tgcgcgacga
     3301 cgaaatcctc acccatccgg tgtttaatcg ctaccacagc gaaaccgaaa tgatgcgcta
     3361 tatgcactcg ctggagcgta aagatctggc gctgaatcag gcgatgatcc cgctgggttc
     3421 ctgcaccatg aaactgaacg ccgccgccga gatgatccca atcacctggc cggaatttgc
     3481 cgaactgcac ccgttttgtc cgccggaaca ggctgaaggt tatcagcaga tgattgcgca
     3541 gctggctgac tggctggtga aactgaccgg ttacgacgcc gtttgtatgc agccgaactc
     3601 cggcgcacag ggcgaatacg cgggcctgct ggcgattcgt cattatcatg aaagccgcaa
     3661 cgaagggcat cgcgatatct gcctgatccc ggcttctgcg cacggaacta accccgcttc
     3721 tgcacatatg gcaggaatgc aggtggtggt tgtggcgtgt gataaaaacg gcaacatcga
     3781 tctgactgat ctgcgcgcga aagcggaaca ggcgggcgat aacctctcct gtatcatggt
     3841 gacttatcct tctacccacg gcgtgtatga agaaacgatc cgtgaagtgt gtgaagtcgt
     3901 gcatcagttc ggcggtcagg tttaccttga tggcgcgaac atgaacgccc aggttggcat
     3961 cacctcgccg ggctttattg gcgcggacgt ttcgcacctc aacctgcata aaactttctg
     4021 cattccgcac ggcggtggtg gtccgggtat gggaccgatc ggtgtgaaag cgcatctggc
     4081 accgtttgta ccgggtcata gcgtggtgca aatcgaaggc atgttaaccc gtcagggcgc
     4141 agtttctgcg gcaccgttcg gtagcgcctc tatcctgcca atcagctgga tgtacatccg
     4201 catgatgggc gcagaagggc tgaaaaaagc aagccaggtg gcaatcctca acgccaacta
     4261 tattgccagc cgcctgcagg atgccttccc ggtgctgtat accggtcgcg acggtcgcgt
     4321 ggcgcacgaa tgtattctcg atattcgccc gctgaaagaa gaaaccggca tcagcgagct
     4381 ggatattgcc aagcgcctga tcgactacgg tttccacgcg ccgaccatgt cgttcccggt
     4441 ggcgggtacg ctgatggttg aaccgactga atctgaaagc aaagtggaac tggatcgctt
     4501 tatcgacgcg atgctggcta tccgcgcaga aatcgaccag gttaaagccg gtgtctggcc
     4561 gctggaagat aacccgctgg tgaacgcgcc gcacattcag aacgaactgg tcgccgagtg
     4621 ggcgcatccg tacagccgtg aggttgcagt attcccggca ggtgtggcag acaaatactg
     4681 gccgacggtg aaacgtctgg atgatgttta cggcgaccgt aacctgttct gctcctgcgt
     4741 accgattagc gaataccagt aattcactga ttcgactatt ttctaaaggc gcttcggcgc
     4801 ctttttagtc agatgacaaa gtacaaaagt gctcagacag tcccctcgcc cctttgggga
     4861 gagggtgagg ggaacaggcc ggcactggcg cgaatattta ccctcacccc ggccctctcc
     4921 ctgaaagggc gagggggaaa agcgtgccaa cattgaagat tgagccagtt tgttagcaat
     4981 ctcaaagata cgtcaacgaa tgaatttttc tcggaaaaac aaatggttat agcacttgtg
     5041 accggtggca gtcgcggcat cgggcgggca actgcattac tgttggcgca agaagggtat
     5101 acggtggcgg ttaattatca gcaaaacctc cacgcggcgc aggaagtgat gaacttaata
     5161 acgcaagccg gtggcaaagc attcgtgctc caggcggata tcagcgacga aaaccaggtc
     5221 gttgcgatgt ttacagcaat cgatcagcac gatgaaccgc tagcagcgct ggtcaataac
     5281 gccgggatct tgtttaccca gtgcaccgtt gaaaacctta ccgcagagcg aatcaaccga
     5341 gtactttcca ccaacgtgac gggatatttt ctctgctgcc gcgaggcggt aaaacgcatg
     5401 gcgcttaaaa atggtggcag tggcggcgct atcgtcaatg tctcttcggt ggcctcacgg
     5461 ttgggttcgc caggggaata tgttgattac gcggcatcga aaggggcgat tgatacgtta
     5521 accaccggac tatcgctgga agtcgccgcg caggggatcc gcgttaactg cgtgcggcca
     5581 gggtttattt ataccgaaat gcacgccagc ggcggcgagc ctggacgcgt cgatcgcgtt
     5641 aagtcgaaca tccccatgca gcgtggtgga caggcagaag aggtcgcgca ggccattgtc
     5701 tggctactaa gtgataaagc ctcttacgtc acgggaagtt ttatcgattt ggcgggcggg
     5761 aaataaaaca gggaagttgt ctgacctgat gcaacaagta ttgcatccgg tacttcatcg
     5821 acttaaagct tctcgccgtt gctggcaatc acctcttggt accagttaaa gctcttctta
     5881 cgtgaacgcg acatatcgcc agcaccgtcg tcatgtttat tcacatagat aaagccgtag
     5941 cgtttgctgt actgcccggt ggtgaacgat acgcagtcga tgcagcccca cggtgtgtag
     6001 cccatcagat ccacgccatc gtaagtcacc gcttttttca tctcttcgat atgggcgcgc
     6061 aggtagtcaa tgcggtagtc gtcgttgatg ctgccatctt cttccacttt gtcgtaagcg
     6121 ccaaaaccgt tttcgacaat aaacagcggc ctctgataac gctcatacag ttcgcaaagg
     6181 gcatagcgca gacctactgg atcaatctgc cagccccagt cagacgcttt aacatacggg
     6241 ttcggtacgc tgccttcgaa accagagatc gcatcgccgg tgccgccttc ggccttcact
     6301 gcgttggtca tgtaatagct gaaaccaaga taatcgcagg tgccttcacg cagtacatcg
     6361 agatcgccgt cttccatttt gatgttaaat ccgcgacgct cccactcgtt caacacatag
     6421 gacgggtaat agccgcgtag ctgcacatcg gtaaagacgt agcgttcgcg catcgactcc
     6481 tgagcgaaca tcacatcgtc cgggttacag cagtagggat agagcggcac catcgccagc
     6541 atacagccga ctttcatctc cgggttaatg cgacgcgcag ctttcaccgc cagggcgctg
     6601 gcgacaaact ggtgatgcag cacctggtac atcgtctctt ccgggttgtc atgctcggta
     6661 taaaccacgc cggagcagca gtaaccgaac agcggtgcac gccagttacg ctggttgtta
     6721 atttcgttga aggtcatcca gtatttgact ttatgcttat agcgttcaaa caccacttcc
     6781 gcgaaacgga caaagaaatc aaccacttta cggttggtcc agctaccgta ttgctgcacc
     6841 agatgcagcg gcatttcaaa gtgggagagg gtgatcaccg gttcgatgtt gtatttcagc
     6901 agttcatcga acatatcatc gtagaacttc agcccttctt cgtttggctg agcttcatca
     6961 ccttttggaa aaatacgcgt ccaggcgatg gaagtccgaa aacatttgaa gcccatttcg
     7021 gcaaatagct tgatgtcttc cttgtagtga ccataaaaat caacggcttc atggtttgga
     7081 tagtatttgc ctggcaagac ttctttggtg atttcgcgcg gcacgccgtg tgcgccaccg
     7141 gtcagaacgt cacaaatgct cggccctttg ccgcctttgt tccagctgcc ttcgacctga
     7201 tgagcggcaa ctgcgccgcc ccataagaaa tctttcggta aggtgagttt tttcactatc
     7261 ataggctcca gatgagtcaa ttcttacagg aaatagtacc atgcagccaa acgacatcac
     7321 tttttttcaa cgtttccagg atgacattct ggctgggcgt aaaaccatca ccatccgcga
     7381 cgagtctgaa tcgcacttca aaacgggtga tgtgcttcgt gtcgggcgtt ttgaagatga
     7441 cggttatttt tgcacgattg aagtcaccgc aacctcaacc gtaaggctgg atacgctgac
     7501 tgaaaaacat gcagagcagg aaaatatgac cctgactgaa ctgaaaaagg tcattgccga
     7561 catctatccc ggtcagacac agttttatgt gattgaattt aaatgtcttt aaattgaact
     7621 cacgcctgtt agttgaaatt tctcatttaa tttcatgatt tttaggagat tagtctcaaa
     7681 tcagaattta ttttagctaa caggtgttca ctggaactat tctcagttac gctggagagg
     7741 tatacatgtg cgtgtacgtt tctccggagt aagttatggt tcagaagccc ctcattaagc
     7801 agggatattc gctggcagag gaaattgcca acagcgtcag tcacggcatt gggttggtgt
     7861 ttggtatcgt tgggctggtg ttgctactgg ttcaggcggt ggatctcaat gccagcgcca
     7921 cagcgataac cagctacagc ctctatggcg gcagtatgat cctgctgttc ctcgcttcga
     7981 cgctctatca cgccattcct catcaacggg caaaaatgtg gctgaagaaa tttgaccatt
     8041 gcgctattta cctgttgatt gccggaacct acacgccgtt tttgctggtg gggctggatt
     8101 ctccgttagc gcgcgggttg atgattgtta tctggagcct ggcattgctg ggtattctgt
     8161 ttaaactgac catcgcgcac cgattcaaaa ttttatctct ggtgacctat ctggcgatgg
     8221 gctggctgtc gctggtggta atttatgaaa tggcagttaa gctcgcggcg ggcagcgtta
     8281 ccttactggc ggtaggcggc gtggtttatt cgctcggggt gattttctac gtctgcaaac
     8341 gcattccata caaccatgcc atctggcacg gcttcgtgct cggcggtagt gtgtgccact
     8401 ttctggcgat ctatttgtat attgggcagg cgtaatgggt atgcctttgt aggcctgata
     8461 agacgcgcca gcgtcgcatc aggcatcgga gcgcaaccgc cggatgcggc gtgaacgcct
     8521 tatccgatct acgggcctcg tccctgttgt gataaaggca tcaaatccca gacatgataa
     8581 gacgcgtcag cgtcgcatca ggcgataaat gcctgatgcg atacagagat ttactcttcg
     8641 agcgaatacg gcagcggctc gatatgcagt gtattcgcat cgtcgcgtac gcggaagatg
     8701 ctgtccggtt ccatatcgtt attcatgacg acctgtacca cgacctgacc atcttccagt
     8761 tttaccgcag ccagcaccgt accggtacga cgccagttct cgcccatttt cagctctaag
     8821 tcttcaccag cttccggcag tcggctggcg ctacctgcca gcaaccagag cgcgcgtttg
     8881 ttggcaccac ggaattttgc tcgcgccacc atctcttgtc cggtataaca gcctttctta
     8941 aagctgatac cgcccagcgc ctggagattg gtcgcctgtg ggataaactg cccgctgttg
     9001 gcggcatcaa tcaccgggaa acccgcttca atgtttaatg ccagccactg ttggctattg
     9061 ttcagttccg cttcaccgcg cagtttatcg gtcagcatat tagcagtagc ttcatcggtt
     9121 acgatcagga aacgttctgc cgggtgttca aaccatagca aagtggtcgc gccttctttg
     9181 actacctgtt tttctttcga aggcagttcg ctaaagagat ttgccagcgc ggcgcgcgcc
     9241 tgaaaaccgg caacaccaag cagcacacgc tcgtcgtctg gcgcgatggt cactttagag
     9301 aataccgcat attttttcag ttcagtcagc tgcggttcac gcacgctgcg ccgttcaatc
     9361 catgcaaagc catcgccgtc gcggaacaga cgtaaattgc tccacatttt acctttggcg
     9421 tcgcaatggg cggcgagcag gtgctgatct tctgccatct ggctgacatc tgctgtcacc
     9481 tgaccctgca tatatttttc gctgtccgcg ccagtaatgg tggcaagcgc ccagtcatca
     9541 agcgtcatca gcgtcagcgg taaacgggca gaagccgtag gctgacgggg aggaaaaggt
     9601 gtaaaagcca taatagagtc ctgagttgct taacggcgta ttaatgacta atggtaaaag
     9661 agctatgagg caatgcaagt gctttcacta cgcgatctgt gccttctgcg aggcgacttc
     9721 caaaaaaatg aagccattga attgttgtga attaatttcg cgatcgcgat ggtgtttact
     9781 gatattcccg taaaagaagg cgaaaaacac gttacaatag cggggtactg gattttcgtg
     9841 agacaggaag aagatggaca ttaacaacaa agcccgcatt cattgggcat gccgccgtgg
     9901 tatgcgcgaa ctcgatattt caatcatgcc gtttttcgaa catgagtacg acagcttaag
     9961 cgatgacgaa aaacgcatct ttattcgtct gctggaatgt gacgatccgg acctgtttaa
    10021 ctggctgatg aatcacggta aaccggccga tgcagaactg gaaatgatgg tccgactcat
    10081 ccagacacgg aaccgggaac gtggtcctgt ggcaatctga tttgcgcgtc tcctggcgcg
    10141 cacagtggct ttccttgctg attcatgggc tggttgccgc tgttatttta ctcatgccct
    10201 ggccactcag ttacaccccg ttatggatgg tgttactttc gctggtggtg tttgattgcg
    10261 ttcgcagcca gcggcgtatt aatgctcgcc agggggaaat tcgcttgttg atggacgggc
    10321 gtttgcgttg gcaagggcag gagtggagca tcggcaaagc accgtggatg attaagagcg
    10381 gcatgatgct gcgtttacgt tctgatggcg gtaaacggca acatttatgg ctggcagccg
    10441 acagcatgga cgaagctgaa tggcgggatt tacggcggat tttgttgcaa caagagacgc
    10501 aaagataaag atgacggcaa agcatatgcg ctaagccgtc agtgagatca ggcgtaatgc
    10561 tctgccattt cgttgaggat ttgctcgcac cagctctgaa tacgctcgtc gctaaggtca
    10621 tactggttag tttcatccag cgccagaccc acgaacagtt gcccgtcagc aatcaccggt
    10681 ttcgggctgg taaattcata tccttccgtt ggccagtagc cgacgaactt cacgcctttg
    10741 gtcgagagtt tgtcatgcag cataccgagc gcatcgagga accactcgcc gtatcccagt
    10801 tgatcgccaa gcccatacag cgcaacaatt ttaccttcaa ggttcaggtc gtcgagctga
    10861 tcccagacgg cttcccagtc ttcctggatt tcaccaaaat cccaggtcgg gatacccaga
    10921 atgagcacat cgtactgctc cattaatttc ggggagtcgt ccttgaggtt atgtaaggtc
    10981 accagttctg ggccgataat atcgcggatt ttttctgccg ccatttcggt gtaacaggtg
    11041 ctggaaccgt aaaaaagacc catattcata acgtaagctt ctcgattctg ctgtggcttt
    11101 tgcagctagt gtatcagaat cgctttagtc acggcacaat aaggcataat ggatcaaacg
    11161 agtgaccata aggggcgcaa gtgaaacagg atctggcacg catcgagcag tttcttgatg
    11221 ctctgtggct ggaaaaaaat ctggctgaaa atacgttgaa cgcttaccgt cgcgatctgt
    11281 caatgatggt ggagtggttg catcaccgcg ggttgacgct ggcgacggcg caaagtgacg
    11341 atttgcaggc attactggca gaacggctgg agggcgggta taaagccacc agctcagcgc
    11401 gtttgctgag tgcagtgcgc cgattgttcc agtatcttta tcgcgaaaag tttcgtgaag
    11461 acgatcccag tgcgcatctc gcttcaccga aattgccaca gcgtttgcca aaagatttaa
    11521 gcgaagcgca ggtcgaacgt ttattacagg caccattaat tgatcagcca ctggagctac
    11581 gcgataaagc catgcttgaa gtgttgtatg ctaccgggct gcgtgtctct gaactggtcg
    11641 gactgacaat gagtgatatc agcctgcgtc agggcgtagt gcgggtcatt ggtaaaggca
    11701 acaaagagcg cctggtgccg ttaggtgaag aggcggttta ctggctggaa acgtatctcg
    11761 agcatgggcg accgtggttg ttgaacggtg tctctattga tgtgttgttc cccagccagc
    11821 gtgcgcagca gatgacgcga cagaccttct ggcaccgcat taaacattat gccgtgctgg
    11881 cgggtatcga cagcgaaaag ctgtcaccgc atgtgttgcg tcacgctttt gccactcact
    11941 tattaaatca tggtgcggat ttgcgcgtgg tgcagatgct actgggccac agcgatctct
    12001 ccaccacgca aatttatacg catgtcgcta ccgagcgtct gcggcaactt catcaacagc
    12061 atcacccgcg ggcgtgatgt ctgaaaagaa cgggaagatt tatgaagaaa ggttttatgt
    12121 tgtttacttt gttagcggcg ttttcaggct ttgctcaggc tgatgacgcg gcaattcaac
    12181 aaacgttagc caaaatgggc atcaaaagca gcgatattca gcccgcgcct gtagctggca
    12241 tgaagacagt tctgactaac agcggcgtgt tgtacatcac cgatgatggt aaacatatca
    12301 ttcaggggcc aatgtatgac gttagtggca cggctccggt caatgtcacc aataagatgc
    12361 tgttaaagca gttgaatgcg cttgaaaaag agatgatcgt ttataaagcg ccgcaggaaa
    12421 aacacgtcat caccgtgttt actgatatta cctgtggtta ctgccacaaa ctgcatgagc
    12481 aaatggcaga ctacaacgcg ctggggatca ccgtgcgtta tcttgctttc ccgcgccagg
    12541 ggctggacag cgatgcagag aaagaaatga aagctatctg gtgtgcgaaa gataaaaaca
    12601 aagcgtttga tgatgtgatg gcaggtaaaa gcgtcgcacc agccagctgc gacgtggata
    12661 ttgccgacca ttacgcactt ggcgtccagc ttggcgttag cggtactccg gcagttgtgc
    12721 tgagcaatgg cacacttgtt ccgggttacc agccgccgaa agagatgaaa gaattcctcg
    12781 acgaacacca aaaaatgacc agcggtaaat aattcgcgtg aaacaacaga tacaacttcg
    12841 tcgccgtgca gtcgatgaaa cggcagactt gcccgctgaa ttgcctccct tgctgcgccg
    12901 tttatacgcc agccggggcg tgcgcagtgc gcaagaactg gaacgcagtg ttaaaggtat
    12961 gttgccctgg cagcaactga gcggcgtcga aaaggccgtt gagatccttt acaacgcttt
    13021 tcgcgaagga acgcggatta ttgtggtcgg cgattttgac gccgacggcg ccaccagcac
    13081 ggctctaagc gtgctggcga tgcgctcgct tggttgcagc aatatcgact atctggtacc
    13141 aaaccgtttc gaagacggtt acggcttaag cccggaagta gtcgatcagg cccatgcccg
    13201 tggcgcgcag ttaattgtca cggtggataa cggtatttcc tcccatgcgg gcgttgaaca
    13261 cgctcgctcg ttgggcattc cggttattgt taccgatcac catttgccgg gcgaaacatt
    13321 acccgcagcg gaagcgatca ttaaccctaa cttgcgcgac tgtaatttcc cgtcgaaatc
    13381 actggcaggc gtgggtgtgg cgttttatct gatgctggcg ctgcgcacct ttttgcgcga
    13441 tcagggctgg tttgatgagc gcggcatcgc aattcctaac ctggcagaac tgctggatct
    13501 ggtagcgctg gggacagtag cggacgtcgt gccgctggac gctaataatc gcattctgac
    13561 ctggcagggg atgagtcgca tccgtgccgg aaagtgccgt ccagggatta aagcgctgct
    13621 ggaagtggca aatcgcgatg cacaaaaact agccgccagc gatttaggtt ttgcgctggg
    13681 accgcgtctc aatgctgccg ggcgactgga tgatatgtcc gttggtgtgg cgctgttgct
    13741 gtgcgacaac atcggcgaag cgcgcgtgct ggcaaatgaa ctcgatgcgc taaaccagac
    13801 gcgaaaagag atcgaacaag gaatgcaggt tgaagccctg accctgtgcg agaaactgga
    13861 gcgcagccgt gacacgctac ccggcgggct ggcaatgtat caccccgaat ggcatcaggg
    13921 cgttgtcggt attctggctt cgcgtatcaa agagcgtttt caccgtccgg ttatcgcctt
    13981 tgcgccagca ggtgacggta cgctgaaagg ttccggtcgc tccattcagg ggctgcatat
    14041 gcgtgatgcg ctggagcgat tagacacact ctaccctggc atgatgctga agtttggcgg
    14101 tcatgcgatg gcggcgggtt tgtcgctgga agaggataaa ttcgaactct ttcaacaacg
    14161 gtttggcgaa ctggttactg agtggctgga cccttcgcta ttgcaaggcg aagtggtgtc
    14221 agacggtccg ttaagtccag ccgaaatgac catggaagtg gcgcagctgc tgcgcgatgc
    14281 tggcccgtgg gggcagatgt tcccggagcc gctgtttgac ggtcatttcc gtctgctgca
    14341 acagcggctg gtgggcgaac gtcatttgaa ggtgatggtc gaaccggtcg gcggcggtcc
    14401 actgctggat ggtattgctt ttaatgtcga taccgccctc tggccggata acggcgtgcg
    14461 cgaagtgcaa ctggcttaca agctcgatat caacgagttt cgcggcaacc gcagcctgca
    14521 aattatcatc gacaatatct ggccaattta gcgtcatctt ctctataaaa aagagcgtgg
    14581 attgggtaca atcccgctct tatcaccgca ttttgactag ctcaataaaa gaaatcagac
    14641 catgtttgaa attaatccgg taaataatcg cattcaggac ctcacggaac gctccgacgt
    14701 tcttaggggg tatctttgac tacgacgcca agaaagagcg tctggaagaa gtaaacgccg
    14761 agctggaaca gccggatgtc tggaacgaac ccgaacgcgc acaggcgctg ggtaaagagc
    14821 gttcctccct cgaagccgtt gtcgacaccc tcgaccaaat gaaacagggg ctggaagatg
    14881 tttctggtct gctggaactg gctgtagaag ctgacgacga agaaaccttt aacgaagccg
    14941 ttgctgaact cgacgccctg gaagaaaaac tggcgcagct tgagttccgc cgtatgttct
    15001 ctggcgaata tgacagcgcc gactgctacc tcgatattca ggcggggtct ggcggtacgg
    15061 aagcacagga ctgggcgagc atgcttgagc gtatgtatct gcgctgggca gaatcgcgtg
    15121 gtttcaaaac tgaaatcatc gaagagtcgg aaggtgaagt ggcgggtatt aaatccgtga
    15181 cgatcaaaat ctccggcgat tacgcttacg gctggctgcg tacagaaact ggcgttcacc
    15241 gcctggtgcg taaaagcccg tttgactccg gcggtcgtcg ccacacgtcg ttcagctctg
    15301 cgtttgttta tccggaagtt gatgatgata ttgatatcga aatcaacccg gcggatctgc
    15361 gcattgacgt ttatcgcgcg tccggcgcgg gcggtcagca cgttaaccgt accgaatctg
    15421 cggtgcgtat tacccacatc ccgaccggga tcgtgaccca gtgccagaac gaccgttccc
    15481 agcacaagaa caaagatcag gccatgaagc agatgaaagc gaagctttat gaactggaga
    15541 tgcagaagaa aaatgccgag aaacaggcga tggaagataa caaatctgac atcggctggg
    15601 gcagccagat tcgttcttat gtccttgatg actcccgcat taaagatctg cgcaccgggg
    15661 tagaaacccg caacacgcag gccgtgctgg acggcagcct ggatcaattt atcgaagcaa
    15721 gtttgaaagc agggttatga ggaaccaaca tgtctgaaca acacgcacag ggcgctgacg
    15781 cggtagtcga tcttaacaat gaactgaaaa cgcgtcgtga gaagctggcg aacctgcgtg
    15841 agcagggaat tgccttcccg aacgatttcc gtcgcgatca tacctctgac caattgcacg
    15901 cagaattcga cggtaaagag aacgaagaac tggaagcgct gaacatcgaa gtcgccgttg
    15961 ctggccgcat gatgacccgc cgtattatgg gtaaagcctc ttttgttacc ctgcaggacg
    16021 ttggcggtcg tattcagctg tacgttgccc gtgacgatct tccggaaggc gtttacaacg
    16081 agcagttcaa aaaatgggat ctcggcgaca tcctcggcgc gaaaggtaaa ctgttcaaaa
    16141 ccaaaaccgg cgaactgtct atccactgca ccgagctgcg tctgctgacc aaagcactgc
    16201 gtccgctgcc ggacaaattc cacggcttgc aggatcagga agcgcgctat cgtcagcgtt
    16261 atctggatct catctctaac gatgaatccc gcaacacctt taaagtgcgt tcgcagatcc
    16321 tctctggcat tcgccagttc atggtaaatc gcggctttat ggaagttgaa acgccgatga
    16381 tgcaggtgat cccaggcggt gcggctgcgc gtccgtttat cacccatcat aacgcgctgg
    16441 atcttgacat gtacctgcgt atcgcgccgg aactgtacct caagcgtctg gtggtcggtg
    16501 gcttcgagcg tgtattcgaa atcaaccgta acttccgtaa cgaaggtatt tccgtacgtc
    16561 ataacccaga gttcaccatg atggaactct atatggctta cgcagattac aaagatctga
    16621 tcgagctgac cgaatcgctg ttccgtactc tggcacagga tattctcggt aagacggaag
    16681 tgacctacgg cgacgtgacg ctggacttcg gtaaaccgtt cgaaaaactg accatgcgtg
    16741 aagcgatcaa gaaatatcgc ccggaaaccg acatggcgga tctggacaac ttcgactctg
    16801 caaaagcgat tgctgaatct atcggcatcc acgttgagaa gagctggggt ctgggccgta
    16861 tcgttaccga gatcttcgaa gaagtggcag aagcacatct gattcagccg accttcatta
    16921 ctgaatatcc ggcagaagtt tctccgctgg cgcgtcgtaa cgacattaac ccggaaatca
    16981 cagaccgctt tgagttcttc atcggtggtc gtgaaatcgg taacggcttt agcgagctga
    17041 atgacgcgga agatcaggcg caacgcttcc tggatcaggt tgccgcgaaa gacgcaggtg
    17101 acgacgaagc gatgttctac gatgaagatt acgttaccgc actggaacat ggcttaccgc
    17161 cgacagcagg tctggggatt ggcatcgacc gtatggtaat gctgttcacc aacagccata
    17221 ccatccgcga cgttattctg ttcccggcga tgcgtccggt aaaataagca ttacgttatg
    17281 ctcacaaccc cggcaaatgt cggggttttt taattgtgct gcgcgaaagt agacaacaat
    17341 tttcttgctt cactattggc tgcctgcatc accatccacg gactgaacgc ccacggcgtg
    17401 gcatcaatac cgtgtaatac atcagctaaa tcacaccatt gataatccat cacttcatca
    17461 tcgttgatct gtaacgcact ggtggtgcgt gcggcaaata ccggacacac ttcattttcc
    17521 acaatgccac tcggatcggt ggcgcggtag cgaaagtcag gatagataga ttcaggaggc
    17581 gtaatttcca cgccaagctc ataacggcaa cggcggatca ccgcgtcttc gctgctttct
    17641 cccagttgtg ggtgcccaca aaccgagtta gtccacacgc caggccacgc ttttttgctc
    17701 agtgcgcggc gggtaactaa taattgcccc ttggcattaa acagccaact ggagaacgcg
    17761 agatgtaaga gggtgtctgc cgtgtgtgcg gcatactttt ccagcgtacc agtgggaact
    17821 ccctgtgcat tcaataaaat gacgtgttcc gtttgcataa tttctcacat gtaattctga
    17881 tcactcatca ttatagataa ttgaagagtg aaatgattaa ccaagtgagc gtttatcgac
    17941 aaccgcccgt tttgagcgga tgccgacagg taaaaaccat ttaaaaaagt agcatcatat
    18001 atttgaagcg actttcaatg gccattgcaa cacagtcaga aatgacagga atagagagag
    18061 taaaaagcaa cagcagactg aatatagcgc gcattaaaag attcatatta ttctccatgc
    18121 tcatttttct tcagcttgcg catcagaagc cacatacgta ctgtcctgac ttttaaatct
    18181 ttatcagaga cggattcctt caccaggtca gcatgatgct gatagccatt aaagaagacg
    18241 ttcagcacaa cggcgctaag ggttgcgagc ataatgccgc tatgtagcag cggttgcagt
    18301 acggccggta acttagaaaa gaaatcgtga gagagcgtcg gagtcatgcc aacgccgaga
    18361 ctgatcgcca caatatagag gttgtaacgg ttggtggtgt agttacagcg cgacagaatt
    18421 cgaatccctg tcgccagtac catgccgaac atcaccagcc cagcaccgcc cagcacaaat
    18481 tgcggaatgg aggcgaccag caccgccatt ttgggcacca tgccaaacag gattaatata
    18541 attcccgaag aaatacacac ccagcggcta tgaacgcgcg tcacgctaac caggccaacg
    18601 ttttgtgaaa atgacgtgtg ggggaagctg ttaaacgttc cgcctatcat tgtccctacg
    18661 ccatcgacac gcagcccgcg aataatatcg tgtgaagaga gtttacgacc gactatttca
    18721 cccagtgcta ggaacatccc catcgactcg ataaacacga tgattaacac ggcagtcatg
    18781 gtcagaatgg aaacgggatc gaaaatcggc ataccaaatg acatcggcgt gacaatcgca
    18841 aaccatgaag catcatgtag cccggataaa ttgacttcat tcatcatcca cgaaagtaaa
    18901 aagccaaata caatccccag taatacggcg acgttggaca taaatccttt cgcatagcga
    18961 gtaatgagca agataaaaat taagacagca aaggagatac ctaaataaac gggattacca
    19021 tattgcggat tccctttacc tccggcggcc cagtcaatac ccacctgaat gatgctaagc
    19081 ccgatagaag taataaccac accggtaacc agtggcggga ataaaggcat caagcgaccg
    19141 ataagtggcg ctaataatgt ggtgataaaa cccgcggcga tagtggcacc aaatatcccc
    19201 agcaggccga tatccgggtt catcccaatg gctatcattg gtgttacagc agcaaaggtc
    19261 accgacataa tcaccggcag gcggatcccc ataaagcggc cgataccgat acattgcaat
    19321 aatgtgacga tcccgcagca aaagagatcc gagctaatga gcatcgcaat agcttctttt
    19381 gagaggccca gtcggtcacc aatcataaga ggaacagcga ctgcacctgc gtacatgaca
    19441 aggacgtgtt gcagaccgag tatgatcagc tttcctggtg ataatatgcg gtcaacctca
    19501 tcagttgggc ggtcttgccc agaagatgag ggaagttggg aatctatggc gctcatcagt
    19561 gtttctcctt tcgcccgcca gagtaaaaag caaccgttta cgttaatgaa actgtctaaa
    19621 cacatgctcg tggagtgcca gtttttgcga ggaaggggag ttagagacag gaattacgga
    19681 ggcaatcaca taatcgtggt taaaccacga aaatgagaag agaacctgac aatgaaatta
    19741 tctgtaattt tctcatattg atatttcatt aatcattctg gcatgtcgca ctctcgcatt
    19801 taatcgtttt tatctggata gcgctctttt ggtcggcaat gttgaaataa tgaaagcggc
    19861 atagattgag gggcaataat ttgaactcag tcgcaaaatt ataattaaaa cggtcatgct
    19921 acctaatata tgcccgttta ttgcatcgcg aaatttgaga agacataact cattaaatga
    19981 aagggatgca aatgaataag tttatcgctg ctgaagctgc ggaatgtata ggctgtcatg
    20041 cttgtgaaat tgcctgtgcg gtggcacaca atcaagaaaa ctggccgctg agtcacagtg
    20101 actttcgacc gcgtatccac gttgtaggga aaggccaggc tgcgaatccg gtggcctgcc
    20161 atcactgcaa caatgcccct tgcgttacgg cttgtccggt taatgctctg actttccagt
    20221 ccgatagcgt acaactggac gagcaaaaat gtattggctg taaaagatgc gcaatcgctt
    20281 gcccctttgg cgtcgttgag atggtcgata cgattgcgca gaaatgcgac ctttgtaacc
    20341 agcgcagttc cggcacgcaa gcctgtattg aagtctgccc aacacaggcg ttacgactga
    20401 tggacgataa agggttacag cagataaagg tggcccgcca gcgcaaaacg gcagcaggaa
    20461 aagcgtcatc agacgctcag ccatctcgca gtgcagcgtt gctccccgtt aactcgcgta
    20521 aaggcgcaga taaaatttca gcgagtgaac ggaaaaccca ctttggcgaa atatattgcg
    20581 ggctggatcc acaacaagcg acttatgaga gtgaccgctg tgtttattgt gccgaaaaag
    20641 ctaactgcaa ctggcattgc ccgctgcata acgctattcc ggattacatc cgtctggtac
    20701 aggaaggaaa gattattgaa gcggcagaac tttgccacca gaccagttcc ttaccagaaa
    20761 tttgcggcag ggtatgtcca caggaccgtc tttgtgaagg cgcatgtact ttgaaagatc
    20821 actcgggcgc agtctctatc ggtaatctgg aacgctacat caccgatacc gcgctggcga
    20881 tgggctggcg tcccgatgtc agcaaagttg ttccccgtat cgaaaaagtg gcggtgattg
    20941 gcgctgggcc agcagggtta gggtgtgctg atattctggc gcgcgcggga gttcaggttg
    21001 atgtctttga tcgccatcca gaaattggcg gtatgctgac ttttggcatt cctcctttca
    21061 aactcgataa aacggtatta agccagcggc gagagatatt caccgcaatg ggaatcgact
    21121 ttcatcttaa ctgtgaaatt ggccgcgata ttacctttag cgatttaact tctgaatatg
    21181 atgcagtttt catcggcgtg gggacttacg ggatgatgcg agcagatctg ccgcatgaag
    21241 atgcgcccgg tgtcattcag gctctaccgt tcctgactgc ccatacccgc cagctcatgg
    21301 gattgccgga gtctgaagag tatccgctga cggacgtgga aggtaagcga gtcgtggtat
    21361 tgggcggtgg cgatacgaca atggattgtt tgcggacttc catccgcctc aatgccgcca
    21421 gcgtgacctg cgcgtatcgt cgtgatgaag tcagtatgcc gggctcgcgc aaagaggtgg
    21481 ttaatgcgcg cgaggaaggt gtcgagtttc agttcaatgt tcaaccgcaa tatatcgctt
    21541 gtgacgaaga tgggcgttta actgcggtgg gcctgattcg taccgccatg ggtgagccgg
    21601 ggccggatgg tcgccgtcgt cctcgtccgg tagcgggttc agagtttgaa ttgcccgccg
    21661 atgttctgat tatggccttt ggtttccagg cgcatgccat gccgtggttg cagggcagcg
    21721 gaattaaact cgataaatgg ggcctgattc aaaccggtga cgtcgggtat ttacctaccc
    21781 agacgcatct gaaaaaagtc tttgctggtg gtgatgcagt tcatggcgcg gatctggttg
    21841 tcactgcaat ggccgcagga aggcaggcgg cgcgcgatat gttaactctg tttgatacga
    21901 aggcatcgtg atgaaatcgt taattatcgt taatccggct gactgtattg gctgccgtac
    21961 ttgtgaagta gcctgtgtgg tcgctcaccc ttcagaacag gagttgaatg ccgatgtctt
    22021 tttaccccgg ctgaaggtac agcgactgga cagcatcagt gcgccggtga tgtgccatca
    22081 gtgtgaaaac gccccttgtg ttggcgcttg ccccgtgggg gcgctgacga tgggcgagca
    22141 ggtggtgcag accaattctg cccgttgtat tggctgtcag agttgcgtca gtgcgtgccc
    22201 gtttggaatg atcaccattc agtcgttgcc gggggatacc cgacaacaaa ttgtgaaatg
    22261 cgatctctgt gaacagcggg aagaggggcc agcctgcgtt gaatcttgcc caacacaggc
    22321 gctgcagttg cttactgaaa gagaactcag gcgagtccgc cagcagcgta ttgttgccag
    22381 cggtgagaat cctctgtgaa agaaatctcc gcgctgaaag gcgcggagaa tctattagat
    22441 tgcccaacca cccgcgtaaa atgcgaccag tgcggcagta atgatcaccg taccaatatt
    22501 cagcttttgc cattcgcgtg caaagacgcg gcctacgacc agtgtcacaa agcccagcat
    22561 aataccggta acgatattac aagtcagaac gatgaacacg gcacacacca ggccagccat
    22621 agcgtcaata aaatcattga aatccagctt cgagacgtta cttaacatca gcaaacctat
    22681 gtacatcaga gcgggtgcag tggcgtaacc agggatcaaa aatgacagcg gtgataagaa
    22741 cagaatcagc aggaataacg cccccactac ggttgcggtt aaccctgttt tacccccggc
    22801 ggcggttcct gccgctgatt cgatataaac cgctgcgggc gctgcgccca ccaggccgga
    22861 gaatattgaa cttactgagt cactggtcag ggctttgccg ccgttgatga tctggttgtc
    22921 tttatccaac aaattcgcct gaccggcgac ggcacggatg gtgccagtag cgtcgaacac
    22981 tgcggtcatc accaatgcca gtacactcgg aagtacggtt ggctggagtg cgcccataat
    23041 atcgaggctg aaaatcagag acttaccatc ttcgccagtc agacttggca tcgccaccag
    23101 accgtggtat ttcaccgctg ggtcaaagat taagccgata atcgaaattg cgataatcac
    23161 caacaagatc ccgccgggta cgcggcactt ctccagaccg aagatgaccg ccagccccag
    23221 caagctcatc atcaccggga aggaggtaaa cgcaccgagc gccactggca agccttcaat
    23281 cgggtttttg ataaccatac ccacaccgtt agcagcaatc agcagcagaa acaggccgat
    23341 accgatacct gtaccgtgag cgatacccat cggtaaatta cgtaagatcc aggtacgcac
    23401 accggttacg gaaatagcgg tgaagatgac gcccatcaga aagaccgcgc ccagtgcgac
    23461 aggaacgcta atttgttgcc cgagtaccag actgaatgcg gtaaatgccg tcaaggaaat
    23521 cgcacaacca atcgccattg gcaaattggc ccacaacccc atcagcaacg agccgaagcc
    23581 cgcgaccaga caggtggcaa caaacacagc tgcgggagga aaacctgctt tgcccagcat
    23641 tcccggaacg acgataacgg aataaaccat ggccagaaag gtcgttaagc cagccagtac
    23701 ttcctgacga acggtactgc cacgagcggt aattttaaaa taattatcca gcgcgccctg
    23761 tggctttggt gcgtccggtg tttgtaggat gtctccagac ataatgatgt cctcacgagg
    23821 atgttaaagt tttattatta gttgcgttcg tacaccagac gaccatcaac gtaggtgcgg
    23881 tagatcgaac ggtcatcgcc caacgtcatc atcacgaaca atttgtcgac taaagaaaca
    23941 gagttgtcat agcgcagctg ctgtagcgga gtggcggtgg gttccatcac cacgaaatca
    24001 gcctctttgc caggtaaaaa gttgccaatc aaatcgtcaa ggcccagaga tttcgctccg
    24061 ccgagcgtgg ccaggtaaaa cgcttcatat gccgagaggc gatagccttg taattgcaat
    24121 actttgtagg cttcgttcag cgtttgcagc atgttgaaag tggttccggc accgatatcc
    24181 gttcccatgc ccactttaac tttcttctgc catgcttttt tcaagttgaa taagccgctg
    24241 ccgaggtaaa ggttggaggt cggacagaaa gcaatgctgg atttggtttc gctgagacga
    24301 tcccactctt tttcttcgag atggacgcag tgagcaaaga cacagttttt accggtcagg
    24361 ccgtactgat ggtaaacatc cagataacca tcatggtcag gataaagcga tttcacccag
    24421 gcaatttcat ctttgttttc acagagatgg gtatgtaccc acgtatccgg atattcttct
    24481 ttcaggcgtt gcgccatcgc catctgttca ggagatgagg tcggggcgaa gcgtggcgta
    24541 atcgcatata gcagacgacc atttttgtgc cagcgttcaa tcagttcttt gctttggtga
    24601 tagctgcttt cggcagtgtc gagcagataa tccggtgcgt tgcggtccat catcacctta
    24661 ccggcaatca tacgcatatt gatatgactg gcggcttcaa acagcgcatc aacagattgc
    24721 ggatgaacag tgccaaacac cagcgccgtg gtggttccgt tacgtaaaag ctgcttgatg
    24781 aagaatgccg acatttcgcg ggcgtactct aaatcctcat aacgacgttc agtagggaag
    24841 gtgtgtttat tcaaccactc cagcaattgt tcaccatagg cccccaccat ttcactttgc
    24901 ggataatgga tatgtgtatc gacaaagccc ggtactatca gtttgccgcg atagtcgcgc
    24961 acgcgaatag tgtcaggaat ttgatgcttt ccgtcttccc attcgccaaa ccattccact
    25021 tttccctgtt taatgagtaa taaaccatcc tcaataaacc gcagcgcaga ggcaatctct
    25081 tccgggttat cgaccgtacg ggtgacatca ataaaactgc ctcgtaccgc tttcaacgtg
    25141 tgttctcctg acatcattga ctcctttact gttaatccat ctcttccgct gaggtaatac
    25201 caggcagaac gttttcctgt cggtagccac cagggaggat aatatttaat aaaatcgcag
    25261 ttaacccgcc agcacaaata gggttttcaa ctagtacata aatagaggct ggcaatattt
    25321 taaaaatttc gggatcgtag gagacgccaa gtcctaaacc taaagaagtg gcgacaataa
    25381 gtgtttctcg gcgctttaaa ccgttggtga tgattatgcg aatcccagcg atggcaatca
    25441 tggaaaacat caacgtcatt gcgcctccca gaactgccga gggaatggtc gtgaagaagc
    25501 cgccaatcat cggaaacaaa ccgaggataa ccagcattac cgcgatggtt cgcccgacat
    25561 aacgtgaagc gacgccagtc atctgaataa ccccattatt ttgcgcaaac gtggttaagg
    25621 gtaatgaccc gacagcggag gcgataacag aaaccagacc gtctgccagc acgccgcctt
    25681 tcagtcggga ctgatactct tctccctgaa tgggtcggcg ggaaaccatt gccgtggcgg
    25741 tgatatcgcc gacagcttcc agcacgctaa gcagataaat cgtgccaacc accaggaact
    25801 gatggaagct aaaactaaag ccgtatttga acggatgcgg gatggtgatt aacggcaaat
    25861 tgcgcatact gctgaaatcc accatgccca ggcataacga tgcgatatag ccgacacata
    25921 gcccaatggc gatccctccc atgcgtagca acggactgcg acagcagtta aagccgatca
    25981 ccacaattaa aaccaataaa ccaacgccga gatgttcgta attgccgaat gtaccgctgc
    26041 ttttggctgc aaatcctcca ccaaaatcga taatgccgac tttaatcagg cttaagccga
    26101 tcatcagtac cacaataccg ctgacggtag gcgtaataac ccgacgtaaa tagggcaaga
    26161 taaatgaaga tccgacaacc agaaatgcgc caacgaagga gacgccgaga agcgacgaca
    26221 tgattaactc ttcgtgaaaa ccgtcgcttt tcatgctgct gcccagcgca atcatgaccg
    26281 taacaaatga aaaattgact gactgaattg agagtaggcc agaaccgacg atgccgtagc
    26341 ggtttacttg taaccaggta ccaataccag aggcgatcat cgccatagaa acaagatagg
    26401 cagttgtttc agcggaaagc tgtaaggccg cccccacgat taacgcgggg gttaccatcg
    26461 gaacgaaaat tgccaacaga tgggtaatgg caccaacgag agcctgatga aagggagggc
    26521 gatcctccag ttcaaatata aggtcagaac ctgcattgtt tatatcagac atcccttcac
    26581 tccttttaaa gtaaatttga tcatctgaat tagtaaaata aattcccacg aatttggggc
    26641 gcaggacgcc ttgagaacgg cttatctcat tttcgcagtc actatttagt catcgtccaa
    26701 acagagaaac aggccgccga agcggccatt tattatttat attttttcca gcgcagtgag
    26761 tattttctcc ggtgtgaaat gccattcgcg taaccagatg ccgcatgcat cgtgaattgc
    26821 ggtagcaatc gccggagctg cgccatttac accgatttcc gagatcgatt tcgccccgaa
    26881 cgggccgact ttatcgtcgc tcggcaccag cacagcgcgg aaatcacgcg gaatgtcacc
    26941 aattttcggt gcgccgtaac tgcgtaaatc acgcgttaac gggtgacctt cggcgtcata
    27001 aatgatctct tcgctcatac tgtggccgat agctcgcagg gtagcaccgt agatttgtcc
    27061 caatgctaat tccgggttga ccggtgtacc gcagtccagc aaggcgtaga atttatccag
    27121 gcggatttca cccgtacgcg tgttgacggc aacttcagcg aagtttgcgc catacgggaa
    27181 ggcgaaatca ggcgtgatat aactgccagt tcccaccagt gaaccaaagc cggtgccggt
    27241 ttcgccttta tgagcaatat ccccgaagct gacttcgcct ttcttgccgc gcacgacgcc
    27301 cggcgttgcc agttgaacat ctgccactgc ctgacccaac atttgcgcgc cgtggaacag
    27361 aattttctcc cgcagatttt ccgctgccaa acgcgccgcg ttacctgaga agcaagtacc
    27421 ggacgaggca tatgcgcctt tatcaaacaa cgcatgatcg gtatcaccgg agataacatg
    27481 cacgtcctgc ggtgggcagt gcagcacttc tgctgccagt ttcgtcacta cagtatccag
    27541 accagtacca atatccgcac cgccagaatg aacgataaag gtgccgtccg attccagttt
    27601 gatcatgcag ttagcctgat cgatatccgg gatacccgat ttctgcatga taatggcgac
    27661 gccgcgaccg atgtgccagt caccattttg cggtttcggt gacgaccatt ggatcatctc
    27721 gcgaccctga cgcaggatct cttctagtgc acagctggcg gcggaaggaa cggaggtcgg
    27781 cgctttacct tcaccgattg caccgagaat tttcagctct tgcccttcgt gtacccggtt
    27841 acgttcgata atttccagtt ggtcgatctg taactgttca gccagttccg ctaatgccat
    27901 ggtgatagcg aagttacctt tcggtgcgcc ataaccctga taagcaccat ttgggcaaat
    27961 gttgctgtag taggtggtga cctggaaatc gacgttatcg cacggatata acggcagcga
    28021 cagcgccggt ccgttacacg gtacggtgag tgagtggttg ccgtaagggc cagtgttggc
    28081 gcggaaatcc atcttcactg ccgtcaggcg accatctttt ttcgctccca gtttgacggt
    28141 gactttcgcg acgtgacgag aggtgttagc aataaactct tcttcacggg tgtagcggaa
    28201 cagtaccgga cgcccggtca cgcaggttgc ccaggcgcac acttcttcca gcaggatgtc
    28261 ctgtttggaa ccaaaaccgc cgccaactcg ctctttaatg acatgaactt tatgctgttt
    28321 catgcccacg aggcgcgcga cctggcggcg taagtgccat ggtacctggg tggaggcgtg
    28381 gataaccaga cgatcgccgt ccatccgggt aaagcagata tgtgtttcag tcgggcactg
    28441 ctgtgcctgc gttgagttat aggttcgctc aatgatcaca tcggcatcgg caaagccttt
    28501 gtccatatcg ccaatatgac catgaatact ggcggcgata tttttgcgag ggcgagaacc
    28561 gatcgggaag ttgatgatca tatgctcgcc gcgctgggct gcatggctgt tatcgtcttc
    28621 cagagtatct ggcgcaccag caacatacac caccggttca tcgtgcacga caggcgcatc
    28681 ttccgccatt gcttcgtcga tcgacattac cggcttaagc acttcatatt caacgtcgat
    28741 gagcttcaat gcttcgagcg caatttcttc actttctgcg acgaccgcag caacacgatc
    28801 gccgacgtga cgcattttct tgccgaacat acggcggtca agcggtgacg gttccggtgc
    28861 gctctgacca cccggggtat agtagatatc cgggcaattc aggtgagtaa taacgtgaac
    28921 gacgcccggt aaggcttcag ctttgctgac atccagatga gtaatcagtg cgtgagcgtg
    28981 tgggctacgt aacattttaa tgacgcaggc gtcagccgtt acgcggtctt caacatagca
    29041 gggtttcgcc tgcaccattt tcgcggcatc agttttagga taatgcttgc caatgacttc
    29101 aaggtcgtca cggaaagtcg gagcgatatc aatggtggcc tgcggattat ttttacgtgc
    29161 aaccgccagt tcaatgacct gatagtattg ttgccagcct gcatcgcggc tgaacaaacc
    29221 agaaagcgcg tcgtcgatct cttcgcgggt aggtgcggcg atgcgatcga gaagatcggt
    29281 gataatcaga gctgcagctg gatcgttata accagactgc accacgccaa catcaaccat
    29341 tgcctgttga accagactta actcgttcca tttgcccaaa gattctgcgg tacgaatatc
    29401 tgccttctct aactgtgcgg caataagcaa ggacgcgtta acgatattac cgttaaagat
    29461 tattgcgtca gaaccggcaa acccgaagcc atcatcactg ttgcgtacag agtgcattcc
    29521 catgttaaac aacagctttt gcacgttttc gcctggatta acggttagct cctgaggcgc
    29581 gccatttaaa gtaaagtgga tgatcatacg gcttcctccc cagcctgttg gcagtcggca
    29641 tacaggtcgg ctaccagaac tcccgtgata tagcgtttat aggccacgct gccccgcaaa
    29701 tcttcctgcg ggaaaatggc gttggcgaca gcctgttcaa gtgcattgcc ttccagattt
    29761 tgtttttcga catcatgcag acgcagtgct ttactggcga cgccatccag cgcaattcgc
    29821 ataccgtcgt ggtctgtcat tgcaacggct gccgtcacga cggttaaacc tgcctgagaa
    29881 cggctaattt tgcgggtcgc acaggtgcga tacggatctt taatgataat ttcggttagc
    29941 aggcgatcgc atgggcaggc caggtagtcc tcgattgaca gcgtttcgcc gttgccaaaa
    30001 accagttcag catccagtgc caacaggacg ggaagcagca ccgactcttc ctggcgggcg
    30061 gcgatttcac caccaatggt cgactgatta cgaacatggc gtgagtaaac aaaaccgagg
    30121 gcttcacgca gcgctgcagg aataaatcgc gcatcacgca gtggctgcaa gcgagacatt
    30181 gcgccaatcc gcagtgcacc gttatcccag tcaacccagt ccagttccag atcctgtaag
    30241 gaaatggcaa tctttttatc ggtacgggtt ggtgtagcat tgagtttgct gcccccggcg
    30301 aaccagacgg cttcatcctg gtagcggcgc ttcagttcca gcgcctgttc gacagagtcg
    30361 ggcctgaaaa attgttcaat catcttcttt ccttatttaa ccctcttccc tgcgggaaga
    30421 gggcatctgt catttatgcc agcgcatcca tccgacgcca catactggca gcggcttttc
    30481 tggcttgtgc ataaatggaa tcgcaatcga agttaaactg acggtcttca tagaccatca
    30541 caccattgac catcacgctg tgaacgctgc ctgagcccat accgaaagcg atatgcccgg
    30601 cgatattgtc tgccagcagc ggcgtcggtg agttgtaatc gcaaatggtt aaatcagctt
    30661 tgtaaccggc ttccagaagt ccaaatttcg cgccaaagtt gcggctcatc agttcgttac
    30721 cgttagtcag ggctttggca aaactgtcag gccacagcgg accacccgca tcgcgatgtt
    30781 taaagaaggc aaatttcatc tcttcaaaca tgtccgaacc aatgccgtcc gttcccaacg
    30841 ccagattgcg gatgtcgcta aggtgatggt tgtagccgac atggttgttc atgtttgaac
    30901 gggcgttatg caccaggaac gcatcgcgct gattgagtag ggcgatgtca tctttcgaca
    30961 agtacagccc atgagcgacc agcgttttgc tatcgatgag atcgaattgc gccagtcgtg
    31021 ccagcaggtc tttgccgtac cagtggtgac tgtaggaaac gtcgtaaagg tcttccgcag
    31081 cgtgaatatg caaaccacgg cctgtggctt tcacggcttc acgtagcatc tccagaccgg
    31141 catctggcac ggtaaacggc gcgtgagcac cgatatgtgc ttccaccaga tacggctcgg
    31201 ttgctgcttt cttcgcctca tcaatctgac gggcgaagcg gatgttttct tctacacctt
    31261 cctgcaactc tttgatgccg ttgttacggt cagtagtttc aaaacaggtc atcgcgcgca
    31321 ggccaacttt taaaaatgcg tcgcgcaatg tggagagcga cccgccgata tacgccggag
    31381 aggcatggtg atcgataacc gatgtacatc cgctcttaat cgcttccagg gaacaaatca
    31441 gcccgctgta atagagcgac tcttcatcaa gggcgcgatc gagccgccac cagagatttt
    31501 tcagcgttga gatgaaatcc gggcaggggg cgatgtttgc cataattccg cgggaaagcc
    31561 ccgagtaaaa atggttgtgc gagcagacaa ttcccggcat cacaatccga ccatgcatct
    31621 ctttgtagct ggcatcgggg tagcgttgcg tcagggcatc gccgatagcg acaatcacat
    31681 cattttcgat agcgatatca acgccttcct gcactttcgc cgggtgtaac tgcactgcgg
    31741 tgacattctt cagaatcaac atgattacac ctccacgcgg cccagcagat aatgatgatg
    31801 ctggtggaca tggctgatga tgcggcacat atcgttcagc tccggtggta cgttgttgaa
    31861 ctgaccttcg ctgtcgatgt ttaacaccca gctttggtta ttcagtcgta ctcgtacccg
    31921 gcaatcttcc acaaggaagc ctgggttgct gctgttatca aagtcttgcg ccaggctgaa
    31981 aacggtgatt ttgtctttgt atggtttacc gttccacgga cagaactgag cgcagttgcc
    32041 gcattcgtta cagtaagcgt cgaggtgcag cgtctggaat ctattctgga agcctgggac
    32101 cgcaatggag acgttggcgc ggttcgggca gacatccaca cacttgctgc aaacgtagtt
    32161 acattcgagg cagcgagcgg cttcctgggc gacaaacgcg tcacgatcgt cactgttcac
    32221 cagagtgata gagatatcgc ctttacgttg atagatttcc gccggattga cgttgttcca
    32281 gtatttatcg ttctggtggg aacggatatt ttcccgacta aggatggcat cggtcgcccg
    32341 acgcgcggtt ccgacagcag cgacaatgga ggatggtccg cgctgtacgt cgccgatcat
    32401 aaagacgtca gtcagacgag tttcgccatt gtggtcgacg tctggccagc cgtttttgtc
    32461 cagcggcacg cccatcgcat tcagggcttc agtatcctgc tgttcaccaa tggcggtgat
    32521 caggctgtct acatgcagtg ttactgtttc gttggtttca accggacggc gacggccttt
    32581 ctcatcgggt tcgccaagcg acataacgcg caaggttaag gtgccatcag catcgaaacg
    32641 ttccggatta ttcaggaaac ggaactccac gccgtcgtgc aacgcttctt catactcttc
    32701 gcgccatgcg ggcatctctt gcagtgaacg acggtaaacg atcgttgctt tttctacgcc
    32761 tggaacgcgt aacgccgcac gagcgcagtc cattgcggtg ttacccgccc cgacaacgac
    32821 cacatgtttg cccagcttga gcgctgtacc cttgttgtat tcacgcagga aggggagtga
    32881 tttccaaaca ttttggttgt cacccgccag tttcacaccg ctatttttat cagtgccggt
    32941 ggcaatcaga acatagtgga agccctgatt ttttaactgt tcaacggtca aatcgggtga
    33001 gcagccatac tcaaatttca cgccgtgatc ggcaacaaaa tcgatatcgt gctgaattaa
    33061 ctcagccgga attcggaact gaggaatgat atttttcacc acgccgcccg cattggcttc
    33121 gcgttcaaac agcgtaaccg gatggcccgc tctggcaagg aagtaacctg ctgccagacc
    33181 cgccggacct gcaccaatca cggcaaccgg atggcgtgaa ccagaaccgg ctggtttgtg
    33241 ccagcgttgc ttatattcat cccaaccttt ttccagcgcg acttttttca gttcgcggat
    33301 attcagcgca ctgtcgtaat ccaggcgggt acagttgtat tggcactggt gatcgcaaat
    33361 atgaccggta atggcgggca gagcgttgcg ttggtagatg agttccagcg cgtcggcata
    33421 gcggtgttcg ccaagcagac ggatgtattc cggaatatct tgcttgatag cgcaggcagt
    33481 aacacagggg gcaacgtagc aatcggtcag cggcaggtct tctgccactt caatacgctc
    33541 ttctggcttc cagtgtttct gggtgtattc catggttaac gcatctgctg ccagtctgtt
    33601 cagtcgttcg acgtcaacat ggtcaagtcc ccaggcgtcg gagccttcca gctcgcgcat
    33661 gcaggcactt aagcgcaaat agccgccagg tttcagcagg tcggttgcca tagtaatagg
    33721 gcgaatacct gtatcaaaaa tatcgcggat agtcagctga ctggcaccac cggaataaga
    33781 aatgggcagt ttgccgtcaa aggcgcgaga gagaactgct gcaacattga tggagagcgg
    33841 gaacagcgca cggcctgaca tatacatctc ttcaccaggc agtgcgcctt tattgttgat
    33901 ggtgccgaga gtgttagtca gttttacgcc aaagccgagt gatttttctt ttgccagtgc
    33961 catcaggcgt tccagcattt ccagtgcttg cgtcagcttg aggtcgtgat caaatgactc
    34021 ttcttttaag cctatgtaac cgaaaccgca gacatcgagg atctcacgaa cacgcgcgta
    34081 tcccagtaag gtcgggttaa gtttcacaaa ggtgttgagc cctttttctt ccagcatgta
    34141 gcggcaaatg gcttcgattt catgcggagg acagccgtgc atggtggaga gggtgacgcc
    34201 ctgcaccata ctggtgggga tgcgagcggg taaggcttgc aagctttcgc gtttttcctg
    34261 caatccgtgg cgggctaaaa atgcgtcatc ctggagtaat ttattcagcg tatcgcgata
    34321 ttgcgcgaat ttcggatggt cagatgcgtc catcatattg tcgatgaact gttgcattgg
    34381 cggttgctta ataccttcga ggttgtaacc gacgctcata ttaaagatga acgatttacc
    34441 ggaatcagaa ggctggaaca tcgcttcgag aaggtgcagg gcaaaccagg ctttgaggta
    34501 ttcatcccag gctttaagca gggtaaattc ggtagaccat tcggtgttaa agcactcgtc
    34561 ttcggcatcg atacagggct tttccagctc caggcggtca agaatttgga cggtttttag
    34621 ttcgatgaag cgtccgccag tcagccagga agtgacaata ttttgcgcga gctgcgtgtg
    34681 cgggccagcg gcagggccga cgggagtggc acaggtttca ccgaagacgc taacagtttt
    34741 accttttaca ggtgagtaaa actgttgctc gggaatacca aagattgagc gttgttgttg
    34801 gtattcatca aatatgcgcg tcaaaagttc ctcaaacgga atgggacgca taatatcccc
    34861 cataaccctc tccttagctt atacagatat cattgagtga ccggtgttga gaaatccggt
    34921 ggtttagggg gatagagcaa caatcgcacc actcttgcca ggtttggcgg gaaatataaa
    34981 aaaatgtgag gcggctcgaa acttaaaggc tttttgtgaa tgaactcaca ggtgccaaat
    35041 gagaattaaa atcactcctg aaaattatta ttgtcagccc atttatcact ttttctcttt
    35101 tctcaaaatc agtgattcct tatcattatg ataaagagtg ctggagagat taaatatttg
    35161 aacgcaatcg tttaactaca ttttctaaat tttagtatac cttttttgcg ctgtaataaa
    35221 gtcgtccgga gtatcaatat ctaaaattat ttctgcattt tctatctcga cggaataatg
    35281 agctcccata aggagtgcct gacgcatatt ggtgacattg ggtcgttgga ttgcctgcat
    35341 caagcatggt tttgacacta aaatcggatg gccggggatg ccattatgga gtggcagtat
    35401 tgcgccatca tttcgtaacg accagatttt tctaaaaata tcgatggtga gggttggcat
    35461 gtcaccgtgg gtgagaaaac aatgttctgt ttgcactgcg ggtactgcgg cctttactga
    35521 tgttagtaaa ccctgcgcat aatctgggtt gtggataata gtgatattgc tctggttcgc
    35581 ataacgttcg tgcagttcat taccacgata gccggtgact aaaataattc ggctacaaaa
    35641 ctgcaacgca tttttgatac ttgtatcaag aattgtcccc tgttgccagg gtaacatcat
    35701 tttccattgc cccattcttg atgataatcc ggcagcggta attatacagt cgatggctga
    35761 catagtggtt ccattattta taggggctaa taacgcctga taattgcgtt acttcctgat
    35821 ggattaagtt ggggataatt tatcccagag aggtcataaa gactcgtgaa aagtatagtt
    35881 gacccatcgg cattagtcat tgatttaggt gcgcagaaac gtcccactgt gatttctgtt
    35941 gttggtgctg gggggaaaac cagcttgctt ttttggctgg cagaactgct ccaggctagc
    36001 ggcaggcgtg tgttaatcac gacgacgaca catatgttta tgccaacgtc tcactggccc
    36061 gtggttttct gtcgtgatcc cgccatgctt cctcatgcgt ctcttacatc ccccatttca
    36121 ttttgttttc acagctggaa agcgaaccag ggaaaagtgc agggatttac gccagaagcg
    36181 attgatgcac tggtgcaacg accagagtgt gacgtaattc tcattgaagc agatggctcg
    36241 cgtggaatgc cgttaaaagc gcctgatgag cacgaacctt gcatacccaa aagcagttgc
    36301 tgcgtgattg ctgtgatggg aggacatact ttgggcgcga aagtgagcac agaaaatgtc
    36361 catcgctggt cgcagtttgc tgatattacc gggttaacac ctgatgcaac cttgcaactg
    36421 agcgatctcg ttgcgctggt tcgccaccct cagggggcgt ttaaaaacgt accgcaaggt
    36481 tgtcggcgag tctggttcat taaccgtttt tctcaatgtg agaatgcgat tgcacaaagc
    36541 gagctccttc aaccgctgca acaacacgac gtagaggcaa tctggctggg cgatatacaa
    36601 gaacatcctg caatcgcgcg cagatttgtg aattagcggc ccgggtattc cgggttactg
    36661 aaaggatatc cattgaggtt ggtatgaata ttttcacaga ggctgcaaaa ctcgaagagc
    36721 aaaattgtcc gtttgcgatg gcgcaaattg ttgatagccg aggctcgact ccccgccatt
    36781 ctgcacaaat gttagtgcgc gccgatggtt ctatcgtcgg tacaattggt ggcggaatgg
    36841 ttgagcggaa ggtgattgaa gagtcacttc aggcattgca ggaacgtaag ccgcgattat
    36901 tccatggacg tatggctcgt aacggtgcgg atgctgtcgg gtcagattgt ggaggtgcaa
    36961 tgtcagtatt tatcagcgtc catggtatgc gtccacgtct ggtgttgatt ggcgcggggc
    37021 atgtcaaccg ggcgatagcc cagagtgcgg cgctattagg atttgatatt gccgttgccg
    37081 atatttatcg cgaaagcctc aatcctgaac tattccctcc atcaaccacg cttctccatg
    37141 ctgagtcgtt tggtgcagca gtggaagcac tggatattcg ccctgataat tttgtcctga
    37201 ttgccacgaa taatcaggat cgtgaagccc tcgacaaact cattgaacag cccattgcat
    37261 ggttggggtt gctggcaagt cgccgcaagg ttcagctttt cctgcgtcaa ttgcgtgaga
    37321 aaggcgtggc tgaagaacat attgcccgtt tacatgcgcc cgttggttac aacataggtg
    37381 cggaaacgcc gcaggagatc gccatcagcg tgttggcaga aatattacag gtgaaaaata
    37441 acgcgccggg tgggctgatg atgaaacctt ctcatccttc cggacaccag ctggtggtga
    37501 ttcgcggtgc gggagatatc gccagtggtg tggcgctacg tctgtatcat gcgggtttta
    37561 aagtgatcat gctggaagtg gaaaaaccga cagtgattcg ttgtacggtg gcgtttgccc
    37621 aggccgtgtt cgatggcgaa atgacggtcg aaggcgtcac tgctcgcctg gcaaccagct
    37681 ctgcggaagc gatgaaactt accgaacgcg gattcatccc tgtgatggta gatcccgcct
    37741 gttcattgct tgatgaactg aaaccgcttt gcgtggtgga cgctatcctg gcgaaacaga
    37801 atttgggaac gcgggcagat atggcaccag taacaatcgc gcttgggccg ggctttactg
    37861 cagggaagga ttgtcatgcg gtaattgaaa caaatcgcgg gcactggctc ggtcaggtga
    37921 tttactctgg ttgtgcgcag gagaataccg gtgttcctgg caatattatg gggcatacca
    37981 cccgacgggt gatccgtgct cctgctgcag gcattatgcg atccaacgtg aaattaggcg
    38041 atctggtgaa agagggcgat gtgattgcct ggattggtga gcatgaaatt aaagcaccgt
    38101 tgacggggat ggtgcgtggc ttgttgaacg acggcttggc agtggttggt ggttttaaaa
    38161 ttggtgatat cgatcctcgt ggtgaaaccg ctgatttctc cagcgtttct gataaagccc
    38221 gggcgattgg cggcggcgta cttgaggcgt taatgatgtt gatgcatcag ggcgtgaaag
    38281 cgacaaaaga agtgctggaa gtggcttaaa tgaaaatggc cggatggtat tacccacccg
    38341 gccttaaaat tactgacaaa tcaccgttcc ggttttgcct tcaatccctt ctttcgcttt
    38401 gctcaatacg gtaatcagtg cttcacgacc tgcgcgagag cgagcaaacg aggcggcagc
    38461 ttccacttta ggcagcatag aacctttcgc aaaatgacct tcttcgataa agcgttcagc
    38521 atcacttaat gacaggcgat cgagccactg ttcattctct ttaccgaagt taatggccac
    38581 tttttctacc gcagtcagaa tgatcagcat atcggcatca atcatttctg ctaaacgggc
    38641 gctggcccag tctttatcga taaccgcgct ggcaccacgc agatggttac cttcacgaat
    38701 aacaggaata ccaccaccgc caacggtgat caccacctgg cctgcatcta ccagagcttt
    38761 aaccgtttct ttttcaataa tatcaaccgg ttttggcgag gcaacaacac ggcgatagcc
    38821 gcgaccagca tcttctttca gagtgtaacc ctgttttgtc agttgctctg cttcttgctc
    38881 ggtaaagaac gaaccgatcg gcttggtcgg gttgaggaat gccggatcgt tagcgtcgac
    38941 ttcgacctga gtaaccagcg ttgctaccgg tttattaatg ccccgagaaa gcagttcttc
    39001 tcttaaggcg ttttgtagat cgtaaccaat ataaccctgg cttaaggcaa cgcagacaga
    39061 catcggcagc attggcgagt gcgcttcagt ttttgcagca gcttcaaagg cctggttaat
    39121 catgcccacc tgcggcccgt taccatgagt gacgataact tcatgtcctt gggcaattaa
    39181 atcaacaatt gcctgagaag taatttttac cgctttcatt tgtccggcca ggtcatcgcc
    39241 cagcgcattc ccgcccaggg cgagaacaat tttcttactc atttttcctc actcatcatt
    39301 cttgattgtg tatagatccc tgacagtggt tagagcacgg gagggacaaa cggtttgcgt
    39361 cgcaggaaac gacctcgccc ggctttgcct gtaaaggtgc cgtcacagaa aatcgtttca
    39421 ccacgggata aggttctgac aatcgcgccc tgacaggtaa aaccctccca tggcgagtag
    39481 tcggcgttgt cgtggagatg gcgatgctga atttgttggc tctgacgtgg gtcgataatc
    39541 accacgtcgc catcggaacc gggcgctaat aatccttttt gcggccacag gccaaacagc
    39601 ctggcgggca ttgcgctggt taattcaaca aagcgttccg gtgttatacg tcccgtcatc
    39661 acgccactgg aaaacagtaa ctgcatgcgg ttttccacac cgggtaagcc atttgggcag
    39721 cgactgaaat cgcctttaga aatttgcagg cgttgagcca tcgagaaggt gcagtgatcg
    39781 gtcgccacca cgtcaatcgc accatcgctg atgccacacc acagtttgtc ctgctcgcgt
    39841 acgttacgca gcggtgggct aagaatgaac ttcatgccgt cttctgtatc gtaactgcgt
    39901 tcgtccaaca ggagatattg tgggcaggtt tcaacccaga ctggctggtg attcgcacgg
    39961 gcaagacgca gataatccag acctaagccg ttagacaggt gcacgatata gagcggggcg
    40021 ttaccggcaa tttgtgccag gttaatcatg cgggcgatgg cttccgcttc gcattccaga
    40081 gggcgactca aggcatgata gcgcggcgcg gttaaccctg cggcgataaa ttccgcccgc
    40141 ttgctggcga tagccgcatc attttccggg tgcacggtgg tcagcgcgcc ggattcatgc
    40201 agacggcgta atgcctgcaa aacctcgtca tcgttgagtt tgtattgata ggttaaatag
    40261 agtttaaaac tgctcagtcc ttcctcgacc atcatcggga tttcgtcgag gattgcgtga
    40321 ttaatgtgct ggatcacacc gtgaaagctg taatcgatga ccgctttatg ggcggcataa
    40381 ccacgataaa cctccagttg atggcgtaac cgacagccgt ttgggccaaa tcccatatgg
    40441 tcaataatgg ttgttgtacc gccacacgca gctgcgcggg taccggtaaa aaaatcatca
    40501 caactgcgcg cgatgccgac atcaatattg aaatgcgtat ggacatccac gccgccaggg
    40561 aaaacgtaac agccagtggc atcaatttct tcatacggga gctgcggcga aatattgttg
    40621 cccaactggc gaacaatccc gctttcaatc agcaaatcct gtttggcttg tccatctgcg
    40681 ttaacgacag tgccgttttt gatcaatacg cgcatagcaa actccaaaaa ccctccggcg
    40741 aaccggaggg gaataagtga agagtaatta ctctgttgcc agccagctta acgggattgc
    40801 agcgtacatc gcggcacagg tcaccaggtg agatttccag gttttttcgt taggtgcatg
    40861 cgcttcaggt tctttacccg ggccaaagcc gatcaccgga atgccgtgac ggcccatgat
    40921 agatacgccg ttagttgaga aggtccactt atcaacaacc ggcgctttgc caaacagccc
    40981 ttcgtaggca ttcaccagtg ctttaacggt gaagtgatct tcttccactt tccaggtcgg
    41041 gaagtagcat tcggttgggt aaaccaggcc agtccaggac ggacggtcgt agttgtacat
    41101 agaaacaacc gcgttagctt tctgtactgc aggcagggcg cggatttcgt ccagcgcgcc
    41161 ttcccaggtt tcgccccagg tcagacggcg gtcaatagag acggcgcagc tatctgctac
    41221 agcgcaacgg cttggggatg tgaagaagat ttcagaaacg gtgagggtgc ctttgccgag
    41281 gaattcgtca taacccagac gttgggagag ttcttgtaat tcgccaagaa tcggacccat
    41341 tttgaaaatg gcgttgtcac cgcgttctgg cgcagaaccg tggcagctaa caccctgaac
    41401 atcaatacga atttccatac gaccgcgctg accacggtat acctggcagt cggttggttc
    41461 ggtactgacc acaaattccg ggcgaatgcc ggattgttca ataatgtact gccagcacag
    41521 accgtcgcag tcttcttcct gcacagtacc ggtaaccagc agggtatatt catcttccag
    41581 accgaggtct ttaatgattt taccggcata aaccatagat gccatgccgc cttcctggtc
    41641 ggaagtaccg cgaccaccga tcagctcatc agtttccatg ccttcgtacg gatcgaagtc
    41701 ccagtttttg atgttgccaa tgccgacggt atcgatatga gcgtccattg ccaccagacg
    41761 cgggccgtgg ccgatataac cgagaacgtt gcccatcggg tcgatttcaa ctttatcgaa
    41821 gccgactttt tccatctctt ctttaatacg atgtactacg cgtttttcgt cgcagctttc
    41881 actgggaata gcaaccatgt cgcgcaggaa gcgagtcatg tccgcctggt aatcttttgc
    41941 tttttcaaga atcagtttga atggaatatt cttagccatt atttttctcc agatttttta
    42001 ctccccgttt tgcagggagt agttccaata gggtgattaa ggtgctacag cgtgtttgcc
    42061 ttcccagaca acttcgcggt agtgcttcac gtcggtgtcg ccttcagtgc tgataaccag
    42121 cactacggca tctttgttca gcgccagttt ttccatcagg ctttgacgtt gcgggtgata
    42181 atgaaccgct gcgagaacgc ccaaaccgac agcgccggat tcaccggaga tgatgcgcgg
    42241 gtcgttgccg tacggattac ccagcacgcg catacctaat gcggcaacgc tgtcctggca
    42301 ggagatgaat tgggtggcgc agttacgtag gatttcccag cccagcgggt taggttcgcc
    42361 acaggccagg cccgccatga tggtggccat atcaccgcca acgttgacga tgtcgccttt
    42421 gacgccggag cgataaatac agtcagcttt gtcaggttca acaataatgc tgtgcagatt
    42481 ttgcgggctg tagacgtcga ccagataacc cagcacacca ccggccattg ctccgacacc
    42541 ggcttgcagc agaacgtgcg tcggggttac gcccatttca cgcatttgct cgacggcttc
    42601 atctgccagg gttgcgtagc cttgcatgat ccaggttggg attttggtgt aaccttccca
    42661 tgccgtgtcc tgtaccactt cccagccgtg ctgctgcgcg tgttgcatgg tcaggcgaac
    42721 ggtatcgtca tagttcatat ccgtgacgat gcactcggca ccgaggttca gaatggcgtc
    42781 aacgcgttcc tgagcagaac ctttcggcat gtaaatcacc gcattctgtc cgagttgctg
    42841 tgctgcccac gccacaccgc gcccgtggtt gccgtcggtg gtggtcgcga aagtcatttt
    42901 ttcgccgatg gcatttttca ggtgctcaaa tgacagcgtt tcgatatcaa gatgatattt
    42961 ttcacacaat aattgagcga tggcgtacgc accgccaagc attttgaagg cgttcagacc
    43021 gaatcgtttt gattcgtctt tgacgagaat tttcttcaca ccaaaaaggt ttgcgagatc
    43081 gtccagcgca caaagcggtg ttgggcgata accagctatt ttctggtgga actggcgcgc
    43141 cagtttggcc tggctttgcg aaaagagcgg tgatgtttcg ccgttgaaaa atttgttatc
    43201 ggcgatatca atcttcaatg agaaaacgga catatcctat ccttttggcc tctggaaata
    43261 gagggccgac cagctgatcg gcccgagggg ttatttcacg cgttcttgcg cttctttcag
    43321 cagttgctcg agcagtgcac ctggtttggc gtatttacgg gacaggatca tcgcggcgat
    43381 gatataaggc ttccagctgg cttctttgta ggtagcgata cggtattttt cgaatacgcc
    43441 ttcagtcact tccccttctt tgcaggatac gccgctgata tcagctggca ggcagtgcat
    43501 gtacagggct tcgccatcac gggtcagttc catcatctct tcagtacaat gccagtcttt
    43561 gtgttgcgcg ttctgtgcca gacactgttt ttccagtgct tttaagcctt cgtgatcgtt
    43621 agcgcgcagc aattcggtac gctgttccat cactttgtaa ggtgcccatg acttcggata
    43681 aacgatgtct gcatctttga atgcttcttc catgctggtg acctgacgga agctaccgcc
    43741 agatgcttta gcattgtttt tcgcgacttc tactacatcc gggatcaggt cgtagccttc
    43801 cggatgggcc agggtgacat ccataccgaa gcgagtcatc agaccgatga tgccttgtgg
    43861 tacagagagc ggtttgccat agcttggaga gtaggcccag gtcatggcga tttttttacc
    43921 tttcaggttt tccagtgaac caaagtgttc acgtaaccac gcgaggtcag ccattgactg
    43981 agtcgggtgg tcaatatcgc attgcaggtt cactaaagcc ggacgctgtg gcagtacacc
    44041 ctgcttgtaa ccgtcatcaa gtgcagcgcc aacttcacgc atataggcgt tgcctgcgcc
    44101 cagatacata tcgtcgcgaa taccaatagc gtcggcgcag aaggagatca tattggcggt
    44161 ttcacgcacg gtttcgccgt gagcgatttg tgatttgcct tcgtcgagat cttgttgtgc
    44221 aaggccgagc aggttaagcg cggaagcata agagaagcgg gtacgagtgg agttgtcgcg
    44281 gaatacggaa atacctaatc cactattaaa gactttggtt gagatgtttt cagcacgcag
    44341 tgcttttaat gctgcggcaa cgtccagtac ttgtttcagt tcatctggcg tctgttccca
    44401 cgttaacaaa aaatctttct cgtgaaggtg agaggtcagc gaattgatat ccttaatcag
    44461 ctcattaaca gttttcatca taaagtcccg gtagtggaat gacaggcaaa gtggtttatt
    44521 tcactgtgac ttgccaaagt gattggtgtt ttcacagcta tacctgggac taacaataag
    44581 tgtgccagaa ttatccacgg ggctgtaaaa gactaatttg acggataatg tgtggggttg
    44641 atcacaaatt gatgccagct tcgggttgtt tgtaaaatac gaaaacgatt ggcttttctt
    44701 atcgcgttat ttatttactg ctaagtatca gaaataagat aaagggacag gctatctctc
    44761 ttcagtttga taaactcacc gccg
//
LOCUS       DAZFQD010000020        44198 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.020, whole genome shotgun sequence.
ACCESSION   DAZFQD010000020 DAZFQD010000000
VERSION     DAZFQD010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 44198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44198
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.020"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(60..1652)
                     /gene="ybiT"
                     /locus_tag="ACHKTW_001232"
     CDS             complement(60..1652)
                     /gene="ybiT"
                     /locus_tag="ACHKTW_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HFO6462294.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFADPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIE"
     gene            1871..2791
                     /gene="ldtB"
                     /locus_tag="ACHKTW_001233"
     CDS             1871..2791
                     /gene="ldtB"
                     /locus_tag="ACHKTW_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFO6462295.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVERKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(2850..3968)
                     /gene="ybiR"
                     /locus_tag="ACHKTW_001234"
     CDS             complement(2850..3968)
                     /gene="ybiR"
                     /locus_tag="ACHKTW_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HFO6462296.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPLAGAMMLTLLLLCWCCFPGKALQYHTGVQTPEWKPRLVWSCLGLYIVFLTALE
                     FKQELWGLVIVAAGFALLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAIGLSQVISNVPSTILLLNYVPPSLLLVWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVILPAN"
     gene            complement(3965..4432)
                     /gene="mntR"
                     /locus_tag="ACHKTW_001235"
     CDS             complement(3965..4432)
                     /gene="mntR"
                     /locus_tag="ACHKTW_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HFO6462297.1"
                     /translation="MSRRAGTPTAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            4618..4746
                     /gene="mntS"
                     /locus_tag="ACHKTW_001236"
     CDS             4618..4746
                     /gene="mntS"
                     /locus_tag="ACHKTW_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HFO6462298.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            5018..6601
                     /gene="opgE"
                     /locus_tag="ACHKTW_001237"
     CDS             5018..6601
                     /gene="opgE"
                     /locus_tag="ACHKTW_001237"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HFO6462299.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGISSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYMVGIFIFVLGVIAIRRKKESEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSDDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGSIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(6650..7165)
                     /gene="ompX"
                     /locus_tag="ACHKTW_001238"
     CDS             complement(6650..7165)
                     /gene="ompX"
                     /locus_tag="ACHKTW_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HFO6462300.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(7218..7283)
                     /locus_tag="ACHKTW_001239"
     CDS             complement(7218..7283)
                     /locus_tag="ACHKTW_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6462301.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            7518..8405
                     /gene="rhtA"
                     /locus_tag="ACHKTW_001240"
     CDS             7518..8405
                     /gene="rhtA"
                     /locus_tag="ACHKTW_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HFO6462302.1"
                     /translation="MPGSLRKMPVWLPIIILLVAMASIQGGASLAKSLFPLVGAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
     gene            8704..9207
                     /gene="dps"
                     /locus_tag="ACHKTW_001241"
                     /gene_synonym="pexB"
     CDS             8704..9207
                     /gene="dps"
                     /locus_tag="ACHKTW_001241"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016722 - oxidoreductase activity, acting
                     on metal ions [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HFO6462303.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            9611..10357
                     /gene="glnH"
                     /locus_tag="ACHKTW_001242"
     CDS             9611..10357
                     /gene="glnH"
                     /locus_tag="ACHKTW_001242"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015599 - ATPase-coupled L-glutamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1903803 - L-glutamine import across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HFO6462304.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFGTEPK"
     gene            10496..11155
                     /gene="glnP"
                     /locus_tag="ACHKTW_001243"
     CDS             10496..11155
                     /gene="glnP"
                     /locus_tag="ACHKTW_001243"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HFO6462305.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            11152..11874
                     /gene="glnQ"
                     /locus_tag="ACHKTW_001244"
     CDS             11152..11874
                     /gene="glnQ"
                     /locus_tag="ACHKTW_001244"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HFO6462306.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALENVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     DPQVLIKNPPSQRLQEFLQHVS"
     gene            11991..14216
                     /gene="ybiO"
                     /locus_tag="ACHKTW_001245"
     CDS             11991..14216
                     /gene="ybiO"
                     /locus_tag="ACHKTW_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
                     /protein_id="HFO6462307.1"
                     /translation="MRWILFILFCLLGAPAHAVSIPGVTTTTTTDSTTEPAPEPDIEQ
                     KKAAYGALADVLDNDTSRKELIDQLRTVAATPPAEPVPKIVPPTLVEEQTVLQKVTEV
                     SRHYGEALSARFGQLYRNITGSPHKPFNPQTFSNALTHFSMLAVLVFGFYWLIRLCAL
                     PLYRKMGQWARQKNRERSNWLQLPAMIIGAFIIDLLLLALTLFVGQVLSDNLNAGSRT
                     IAFQQSLFLNAFALIEFFKAVLRLIFCPNVAELRPFTIQDESARYWSRRLSWLSSLIG
                     YGLIVAVPIISNQVNVQIGALANVIIMLCMTVWALYLIFRNKKEITQHLLNFAEHSLA
                     FFSLFIRAFALVWHWLASAYFIVLFFFSLFDPGNSLKFMMGATVRSLAIIGIAAFVSG
                     MFSRWLAKTITLSPHTQRNYPELQKRLNGWLSAALKTARILTVCVAVMLLLSAWGLFD
                     FWNWLQNGAGQKTVDILIRIALILFFSAVGWTVLASLIENRLASDIHGRPLPSARTRT
                     LLTLFRNALAVIISTITIMIVLSEIGVNIAPLLAGAGALGLAISFGSQTLVKDIITGV
                     FIQFENGMNTGDLVTIGPLTGTVERMSIRSVGVRQDTGAYHIIPWSSITTFANFVRGI
                     GSVVANYDVDRHEDADKANQALKDAVAELMENEEIRGLVIGEPNFAGIVGLSNTAFTL
                     RVSFTTLPLKQWTVRFALDSQVKKHFDLAGVRAPVQTYQVLPAPGAAPAEPLPPGEPT
                     L"
     gene            complement(14213..15139)
                     /gene="rlmF"
                     /locus_tag="ACHKTW_001246"
     CDS             complement(14213..15139)
                     /gene="rlmF"
                     /locus_tag="ACHKTW_001246"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008988 - rRNA
                     (adenine-N6-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HFO6462308.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVAFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            15415..15675
                     /gene="mcbA"
                     /locus_tag="ACHKTW_001247"
     CDS             15415..15675
                     /gene="mcbA"
                     /locus_tag="ACHKTW_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family periplasmic protein McbA"
                     /protein_id="HFO6462309.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMFGTATIYK"
     gene            15940..18222
                     /gene="fiu"
                     /locus_tag="ACHKTW_001248"
     CDS             15940..18222
                     /gene="fiu"
                     /locus_tag="ACHKTW_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
                     /protein_id="HFO6462310.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQTNADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNVPGVG
                     AFFAGENGNSTTGDAIYMRGADTSNSIYIDGIRDIGSVSRDTFNTEQVEVIKGPSGTD
                     YGRSAPTGSINMISKQPRNDSGIDASASIGSAWFRRGTLDVNQVIGDTTAVRLNVMGE
                     KTHDAGRDKVKNERYGVAPSIAFGLGTANRLYLNYLHVTQHNTPDGGIPTIGLPGYSA
                     PSAGTAALNHSGKVDTHNFYGTDSDYDDSTTDTATMRFEHDINDNTTIRNTTRWSRVK
                     QDYLMTAIMGGASNITQPTSDVNSWTWSRTANTKDVSNKILTNQTNLTSTFYTGSIGH
                     DVSTGVEFTRETQTNYGVNPVTLPAVNIYHPDSSIHPGGLTRNGANANGQTDTFAIYA
                     FDTLQITRDFELNGGIRLDNYHTEYDSATACGGSGRGAITCPAGVAKGSPVTTVDTAK
                     SGNLVNWKAGALYHLTENGNVYINYAVSQQPPGGNNFALAQSGSGNSANRTDFKPQKA
                     NTSEIGTKWQVLDKRLLLTAALFRTDIENEVEQNDDGTYSQYGKKRVEGYEISVAGNI
                     TPAWQVIGGYTQQKATIKNGKDVAQDGSSSLPYTPEHAFTLWSQYQATDDISVGAGAR
                     YIGSMHKGSDGAVGTPAFTEGYWVADAKLGYRVNRNLDFQLNVYNLFDTDYVASINKS
                     GYRYHPGEPRTFLLTANMHF"
     gene            18264..18941
                     /gene="ybiX"
                     /locus_tag="ACHKTW_001249"
     CDS             18264..18941
                     /gene="ybiX"
                     /locus_tag="ACHKTW_001249"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HFO6462311.1"
                     /translation="MMYHIPGVLSPQDVARFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSTLYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSLHCV
                     TPVTRGVRVASFMWIQSMIRDDKKRAMLFELDNNIQSLKSRYGENEEILSLLNLYHNL
                     LREWSEI"
     gene            19015..19281
                     /gene="ybiI"
                     /locus_tag="ACHKTW_001250"
     CDS             19015..19281
                     /gene="ybiI"
                     /locus_tag="ACHKTW_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HFO6462312.1"
                     /translation="MASGWANDDAVNEQINSTIEDAIARARGEIPRGESLDECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDSQLR"
     gene            19546..19806
                     /gene="ybiJ"
                     /locus_tag="ACHKTW_001251"
     CDS             19546..19806
                     /gene="ybiJ"
                     /locus_tag="ACHKTW_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YbiJ"
                     /protein_id="HFO6462313.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(19909..19968)
                     /gene="ybiE"
                     /locus_tag="ACHKTW_001252"
     CDS             complement(19909..19968)
                     /gene="ybiE"
                     /locus_tag="ACHKTW_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbiE"
                     /protein_id="HFO6462314.1"
                     /translation="MRLSANRQGNTCAGRSVNA"
     gene            complement(20035..21120)
                     /gene="hcxB"
                     /locus_tag="ACHKTW_001253"
     CDS             complement(20035..21120)
                     /gene="hcxB"
                     /locus_tag="ACHKTW_001253"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HFO6462315.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKTVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCAAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCNAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQKQGIPLDAGS
                     WQAICDAARQIGMPEETLQAFCQQLAS"
     gene            complement(21261..22223)
                     /gene="ybiB"
                     /locus_tag="ACHKTW_001254"
     CDS             complement(21261..22223)
                     /gene="ybiB"
                     /locus_tag="ACHKTW_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HFO6462316.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALRLSSVSHPEYIGRVAKFFSDI
                     GGRALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDTAGSELLPQAKDPETTAQ
                     WIERCLAGSEPIPESLKIQMACCLVATGEAATISDGLARVNQAF"
     gene            complement(22251..24401)
                     /gene="dinG"
                     /locus_tag="ACHKTW_001255"
     CDS             complement(22251..24401)
                     /gene="dinG"
                     /locus_tag="ACHKTW_001255"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HFO6462317.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAINDDLWRRLSTDKASCLNRNCYYYRECPFFVARREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDEVLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEAQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRVE
                     PSIDNEEQHIAEMAAFFRKQVESKKHLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            24521..25003
                     /gene="ybiA"
                     /locus_tag="ACHKTW_001256"
     CDS             24521..25003
                     /gene="ybiA"
                     /locus_tag="ACHKTW_001256"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosidase YbiA"
                     /protein_id="HFO6462318.1"
                     /translation="MPVRAQRIQHVMQDTIINFYSTSDDYGDFSNFAAWPIKVDGKTW
                     PTSEHYFQAQKFLDEKYREEIRRVSSPMVAARMGRDRSKPLRKNWESVKEQVMRKALR
                     AKFEQHPELRALLLATAPAKLVEHTENDAYWGDGGNGKGKNRLGYLLMELREQLAIEK
                     "
     gene            complement(25235..26599)
                     /gene="rhlE"
                     /locus_tag="ACHKTW_001257"
     CDS             complement(25235..26599)
                     /gene="rhlE"
                     /locus_tag="ACHKTW_001257"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
                     /protein_id="HFO6462319.1"
                     /translation="MSFDSLGLSPDILRAVAEQGYREPTPIQQQAIPAVLEGRDLMAS
                     AQTGTGKTAGFTLPLLQHLITRQPHAKGRRPVRALILTPTRELAAQIGENVRDYSKYL
                     NIRSLVVFGGVSINPQMMKLRGGVDVLVATPGRLLDLEHQNAVKLDQVEILVLDEADR
                     MLDMGFIHDIRRVLTKLPAKRQNLLFSATFSDDIKALAEKLLHNPLEIEVARRNTASD
                     QVTQHVHFVDKKRKRELLSHMIGKGNWQQVLVFTRTKHGANHLAEQLNKDGIRSAAIH
                     GNKSQGARTRALADFKSGDIRVLVATDIAARGLDIEELPHVVNYELPNVPEDYVHRIG
                     RTGRAAATGEALSLVCVDEHKLLRDIEKLLKKEIPRIAIPGYEPDPSIKAEPIQNGRQ
                     QRGGGGRGQGGGRGQQQPRRGEGGAKSASAKPAEKPSRRLGDAKPAGEQQRRRRPRKP
                     TAAQ"
     gene            26828..27499
                     /gene="cecR"
                     /locus_tag="ACHKTW_001258"
     CDS             26828..27499
                     /gene="cecR"
                     /locus_tag="ACHKTW_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HFO6462320.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            27502..28497
                     /gene="hlyD"
                     /locus_tag="ACHKTW_001259"
     CDS             27502..28497
                     /gene="hlyD"
                     /locus_tag="ACHKTW_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HFO6462321.1"
                     /translation="MKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGNEAGHE"
     gene            28490..30226
                     /gene="ybhF"
                     /locus_tag="ACHKTW_001260"
     CDS             28490..30226
                     /gene="ybhF"
                     /locus_tag="ACHKTW_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFO6462322.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGNATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKEATPD
                     DIRHAEGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGIDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            30219..31352
                     /gene="ybhS"
                     /locus_tag="ACHKTW_001261"
     CDS             30219..31352
                     /gene="ybhS"
                     /locus_tag="ACHKTW_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO6462323.1"
                     /translation="MSNPILSWRRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQAGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            31363..32469
                     /gene="ybhR"
                     /locus_tag="ACHKTW_001262"
     CDS             31363..32469
                     /gene="ybhR"
                     /locus_tag="ACHKTW_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO6462324.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(32431..32841)
                     /gene="ybhQ"
                     /locus_tag="ACHKTW_001263"
     CDS             complement(32431..32841)
                     /gene="ybhQ"
                     /locus_tag="ACHKTW_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HFO6462325.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            32974..33735
                     /gene="ybhP"
                     /locus_tag="ACHKTW_001264"
     CDS             32974..33735
                     /gene="ybhP"
                     /locus_tag="ACHKTW_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFO6462326.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRVHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            33732..34973
                     /gene="clsB"
                     /locus_tag="ACHKTW_001265"
     CDS             33732..34973
                     /gene="clsB"
                     /locus_tag="ACHKTW_001265"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HFO6462327.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DDVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     ARREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRHFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLAQVDP
                     PAQPTMETQDRVETENTGVKP"
     gene            34973..35929
                     /gene="ybhN"
                     /locus_tag="ACHKTW_001266"
     CDS             34973..35929
                     /gene="ybhN"
                     /locus_tag="ACHKTW_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HFO6462328.1"
                     /translation="MSKSHPRWRLAKKILTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNRVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLPGSTITRIFSLSITTNWLGYILLAGIIFTAGVVELPDHWYVD
                     QTTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWKFALAQMLISSVNWMV
                     MGAIIWLLLGQSVNYFFVLGVLLVSSIAGVIVHIPAGIGVLEAVFIALLAGEHTSKGS
                     IIAALLAYRVLYYFIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(35965..36677)
                     /gene="ybhM"
                     /locus_tag="ACHKTW_001267"
                     /pseudo
     CDS             complement(35965..36677)
                     /gene="ybhM"
                     /locus_tag="ACHKTW_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706665.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
     gene            complement(36882..37586)
                     /gene="ybhL"
                     /locus_tag="ACHKTW_001268"
     CDS             complement(36882..37586)
                     /gene="ybhL"
                     /locus_tag="ACHKTW_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFO6462329.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            complement(37723..38175)
                     /gene="moaE"
                     /locus_tag="ACHKTW_001269"
     CDS             complement(37723..38175)
                     /gene="moaE"
                     /locus_tag="ACHKTW_001269"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HFO6462330.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDQQAAKRW"
     gene            complement(38177..38422)
                     /gene="moaD"
                     /locus_tag="ACHKTW_001270"
     CDS             complement(38177..38422)
                     /gene="moaD"
                     /locus_tag="ACHKTW_001270"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HFO6462331.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADFPTVEALRQHLAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHSLTDGDEVAFFPPVTGG"
     gene            complement(38415..38900)
                     /gene="moaC"
                     /locus_tag="ACHKTW_001271"
     CDS             complement(38415..38900)
                     /gene="moaC"
                     /locus_tag="ACHKTW_001271"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061799 - cyclic pyranopterin
                     monophosphate synthase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HFO6462332.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            complement(38903..39415)
                     /gene="moaB"
                     /locus_tag="ACHKTW_001272"
     CDS             complement(38903..39415)
                     /gene="moaB"
                     /locus_tag="ACHKTW_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HFO6462333.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDKAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREIEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            complement(39437..40426)
                     /gene="moaA"
                     /locus_tag="ACHKTW_001273"
     CDS             complement(39437..40426)
                     /gene="moaA"
                     /locus_tag="ACHKTW_001273"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HFO6462334.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEDDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     regulatory      complement(40414..40558)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            40823..41731
                     /gene="yvcK"
                     /locus_tag="ACHKTW_001274"
     CDS             40823..41731
                     /gene="yvcK"
                     /locus_tag="ACHKTW_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HFO6462335.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPSVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDTHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHNALEKALQALG"
     gene            complement(41923..43944)
                     /gene="uvrB"
                     /locus_tag="ACHKTW_001275"
     CDS             complement(41923..43944)
                     /gene="uvrB"
                     /locus_tag="ACHKTW_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFO6462336.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVVVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMNELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDKITPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMSPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
BASE COUNT        10607 a        11748 c        11213 g        10630 t
ORIGIN      
        1 attttgcatt tgtcatcagc cgccgtcacc ccgacagtat cggggtgacg gataatcact
       61 tactcgatcc ctttactacg caggtaatct tcgtaattac cgctaaagtc gatcacgcgt
      121 tccggggtga tttccagaat gcgggtcgcc agggagctta cgaactcacg gtcgtgtgaa
      181 acaaagatca gcgtgccctg atacagttcc agcgccatgt tcagtgactc gatggattcc
      241 atatccaggt ggttggtcgg ttcgtccatg atcagaatgt tcggcttctg catcattaac
      301 ttaccaaaca gcatccgccc tttttcccca ccggaaagca ctttagctgg ctttttgatg
      361 tcgtcctggc tgaacagcaa acgaccgaga atgctgcgta ccgcctgctc gtcatcgcct
      421 tcctgcttcc actgactcat ccattcgaac acggtcagat cattttcaaa ctcatattcg
      481 tggtcctggg cgtagtaacc aatgcgcgcg ttttcagacc attttacggt gccgctgtcc
      541 ggttgcagat cgcccaccag cgttttcagc agcgttgatt taccgacgcc gttggtaccc
      601 agtaccgcaa gtttttcacc gacctccagc agcaggttga gatttttaaa cagtggaccg
      661 ttatcaaacc ctttggtcag accttccact tccagcgcgt tacggaacag tttcttatcc
      721 tgttcaaaac ggatgaacgg gttctgacgg ctggaggctt tcacctcttc cagtttgatt
      781 ttatcaatct ggcgcgcgcg cgaagttgcc tggcgagatt tcgaggcgtt ggcgctaaag
      841 cggctaacga aagattgcaa ctcagcaatc tgcgctttct tcttggcgtt atcggccagc
      901 agacgttcac gcgcctgggt cgccgccgtc atgtactcat cgtagttacc cggataaacg
      961 cgcagctcgc cgtaatccag atccgccatg tgggtacaga ccatgttaag gaagtgacgg
     1021 tcgtgcgaga tgatgatcat ggtgctgtca cgctcgttca gcacctgttc cagccagcga
     1081 atggtgtcga tgtcgaggtt gttggtcggt tcgtcgagca ggagaatatc cggatcagca
     1141 aacagcgcct gcgccagaag cacacgcagc ttccagccag gagcaacttc actcatcggg
     1201 ccgtagtgct gttccactgg aattcccacg ccaagcaaca attcaccggc gcgagcttcc
     1261 gcagagtaac cgtccatttc gccgtattta acttccagat cggccacttt atagccgtct
     1321 tcctcactca tttccggcaa agcatagatg cggtcacgct cctgcttcac ttcccacaac
     1381 tctttatgcc ccatgatcac cgtatccagc acagtgaact cttcaaaggc aaactgatcc
     1441 tgacgcagct taccaatgcg ctcgttggga tcgagggaaa cgttacccaa agtcggctct
     1501 aagtcgccgc cgaggatctt cataaaggtg gatttaccac taccgttcgc gccaatcagg
     1561 ccgtaacggt tgccgccgcc aaatttgacg gaaatgtttt caaacaacgg cttactgccg
     1621 aactgcatgg tgacgttact ggaaactaac acaggcgtat cctgaaaaga gatatgacaa
     1681 accgcgcatt atgccataag tctgaaagaa gatcctgctc ctcgcttaag gtctacactc
     1741 aagccaaagg caaaaaatgt gatttcgtac acatctgatt tcactgtgag ctggaatgaa
     1801 cttataatgc gcttccaata ctctaatatt ctcaacccaa tggcctgcca ggcacaaaat
     1861 ctcgcttaac atgaatatga aattgaaaac attattcgca gcggccttcg ctgttgtcgg
     1921 cttttgcagt accgcctctg cggtaactta tcctctgcca accgacggga gtcgcctggt
     1981 tggtcagaat caggtgatca ccattcctga aggtaacact cagccgctgg agtattttgc
     2041 cgcggagtac cagatggggc tttccaatat gatggaagcg aacccgggtg tggatacctt
     2101 cctgccgaaa ggcggtactg tactgaacat tccgcagcag ctgatcctgc cggataccgt
     2161 tcatgaaggc atcgtcatta acagtgcaga gatgcgtctg tattactatc cgaaagggac
     2221 caacaccgtt atcgtgctgc cgatcggcat tggtcagtta ggcaaagata cgcctatcaa
     2281 ctggaccacc aaagttgagc gtaagaaagc aggcccgacc tggacgccga ccgccaaaat
     2341 gcacgcagag taccgcgctg cgggcgaacc gcttccggct gtcgttccgg caggtccgga
     2401 taacccgatg ggtctgtatg cactctacat cggtcgcctg tatgctatcc atggcaccaa
     2461 cgccaacttc ggtatcggcc tgcgtgtaag tcatggttgt gtgcgtctgc gtaacgaaga
     2521 catcaaattc ctgttcgaga aagtaccggt cggtacccgc gtacagttta ttgatgagcc
     2581 ggtaaaagcg accaccgagc cagacggcag ccgttatatt gaagtccata acccgctgtc
     2641 taccaccgaa gcccagtttg aaggtcagga aattgtgcca attaccctga cgaagagcgt
     2701 gcaaacagtg accggtcagc cagatgttga ccaggttgtt cttgatgaag cgattaaaaa
     2761 ccgctccggg atgccggttc gtctgaatta atcttcaaaa cttaaagcaa aaggcggact
     2821 cataatccgc cttttttatt tgccagaccc tagttggccg ggagtataac taacaaaaca
     2881 tatcccacca acgccgccca caacagcatc ggtattgaat agagatggaa acgccaccag
     2941 atgcggcgat cgttcgccat acgtagcgca atcaaatttg ccagcgatcc gggtaataac
     3001 ccaaagccac ctacgtttac cgcccatacc agtaataaag acggcggcac atagttcagc
     3061 aacaatatgg tactcggcac gttactgatc acctgcgata aaccgattgc cgttaaccat
     3121 aacccgggtt cagatagatg actcacgtta cccaacacgc cttgcaacgc tggaagctgg
     3181 gtcagtaaat ggacgtcgat aaacatcgcc ataaacacca gcagcagcgt ccagtccaca
     3241 ctgagcacca cgcgacgtgc cagcagcgca aagcctgccg ccacaatcac cagtccccac
     3301 agctcttgtt tgaactccag cgccgtcaga aagacgatat acagccccag acaactccac
     3361 accagccgcg gtttccactc cggtgtttgc acccccgtat ggtattgcag cgcctttcca
     3421 gggaaacaac accagcacag gagcaggagc gtcagcatca ttgcgccagc cagcggtgcc
     3481 atttgggcaa taaatccggc aaacgaaaga ccagaacgtc cccagataag aatattttgc
     3541 gggttgccaa ttggcgtcag tagcgaacca gcgttgactg ccagcgcctc aaaaataatc
     3601 agccgattaa ccgggatctc acacagtctt tttagcgtga tagtcagcgg aacaacaata
     3661 aacagcgcga catcgttggt cagaaaggta gaaagcagcg ccgccgccag caccataaac
     3721 atcgccagcc gacgctccgt agcaaagcgg cgcaccattt tgcgccccag cacatcaaaa
     3781 taaccgctta actccacacc tttggtcagc agcatcaggc cgcttaaggt gatgatggtg
     3841 tgccagtcga tagcagcagg ccaggatttc ggtgcaaagg gcacgaaaaa gcttaatccg
     3901 ataccaacaa gaattaataa ctgaaaaaaa cgatcgcctt gcagcgtgcg taaaaaaggc
     3961 aggctcattt ggcaccgtgt ttctgggtaa acaaacgaaa agcgtcgagc gtctcttcac
     4021 taacatggtg ctccatgcct tccgcgtcgc gacgggcgat ttccggactg acgcccaaca
     4081 ccaacaagaa attttcgact atctgatgtc gctcgcggct ttcctgcgcc agcttctctc
     4141 cttctgccgt taaaaacacg ccacgccagg ggatcatttc aatcagcccc atggttgcca
     4201 gccgcttaag cattttagcc accgtcggtt gcgaaactcc cagacgagca gccatgtcca
     4261 cctgacgagc ttcccccact tccctgatca agtcagaaat cagctcaacg taatcatcaa
     4321 taagctcgcg ccgatgcgcc tctctgacct gacggaaccc ttcaacgtgc tcttccacgt
     4381 tcactaactg cgtcactttt tttgctgttg gcgtacctgc gcgacgactc attgtgcttc
     4441 ctcattatgg tgacgcatac atagcgttct catttcgtgc gcacattgta aaccagagtt
     4501 gcgaaggtac aaaaaattaa cgttttagca atagctatat aatatagcct gtgctatatc
     4561 tgtatgtaat gcaatcatcc ctcaaggatc gacgggatta gcaagtcagg aggtcttatg
     4621 aatgagttca agaggtgtat gcgcgtgttt agtcattctc cctttaaagt acggttaatg
     4681 ctgctctcta tgttgtgcga tatggtcaac aacaaaccgc agcaagataa accttccgat
     4741 aaatagcggc gtcgcggtac gccgcttcac tcctgctttc atgcaggcat aacgcgtttt
     4801 ggtctgaaaa accccacttt ttgtcggatt tgcaatcccc ttcgcaaaag atttgttcgt
     4861 cagtagttga cctgaacggc ggctcgctct atcttcttgc agccctgcgt atattgcggc
     4921 tcgcggatgc ggaccccttt cccctcttca cgcactcttg caggtattga cccttgacgc
     4981 cagggtaagc acatggcgtt tgttacgata gtggcatatg aatttaaccc tcaaagaatc
     5041 gcttgttacc cgtagccggg tatttagccc gtggactgcg ttctactttt tacagtcgct
     5101 attaattaac ctcggcttag gttacccctt cagtttgctc tacaccgctg cgtttacggc
     5161 tattttgctt ttgctatggc gaacattgcc tcgcgtacaa aaagttctgg tcggtatcag
     5221 ttcgctggtg gcggcttgtt atttcccttt tgctcaggcc tacggcgcgc ctaacttcaa
     5281 tacattgctg gcattgcact ccaccaatat ggaagagtcg accgaaatcc tgacgatttt
     5341 tccgtggtac agctacatgg tcggcatatt tatttttgtg ctcggcgtaa tagcaatcag
     5401 gcgaaaaaaa gagagtgaaa aagcgcgctg gaataccttc gacagcctgt gtctggtatt
     5461 cagtgtggcg acattttttg ttgctcccgt gcaaaacctg gcctggggtg gcgtatttaa
     5521 actgaaagat actggctatc cggtatttcg ttttgctaag gatgtcatcg tcaataataa
     5581 cgaggtgatt gaagagcaag aacggatggc aaaactttcc ggaatgaaag atacctggac
     5641 ggtcactgcc gttaagccga agtatcagac ctatgtggtg gtgatcggtg aaagcgcgcg
     5701 tcgcgatgcc ctcggtgcct ttggcggtca ctgggacaat accccgtttg ccagcagcgt
     5761 taacggtttg atatttgctg actacattgc cgccagtggc tccacgcaga aatcgcttgg
     5821 cttaacgctc aatcgcgtag tcgatggcaa accacagttt caggataact ttgtcaccct
     5881 ggcaaatcgc gcgggcttcc agacctggtg gttttccaac cagggccaaa tcggcgaata
     5941 cgatactgct atcgccagca tcgccaaacg tgcagatgaa gtgtacttcc tgaaagaagg
     6001 taattttgaa gcagataaaa acacgaaaga cgaagcgtta ctggatatga ccgctcaagt
     6061 gctggcgcaa gagcactcgc aaccgcagct gattgttcta catctgatgg gctcacatcc
     6121 gcaggcctgc gacaggacac aaggcaaata cgaaaccttt gtgcagtcga aagaaacgtc
     6181 gtgctatctc tataccatga cgcaaacgga cgatttactg cgcaagctgt acgatcagtt
     6241 acgcaacagc ggcagcagct tctcgctggt ttacttttct gaccacggtc tggcctttaa
     6301 agagcgcggc aaagacgtgc aataccttgc ccatgatgat aagtatcagc aaaatttcca
     6361 ggtgcctttt atggtcattt ccagcgacga taaagcgcat cgggtgatta aagcccgccg
     6421 ctcagccaat gacttcttag gttttttctc ccagtggacg ggaattaaag cgaaggaaat
     6481 taacatcaaa tacccgttta tatctgagaa gaaagccggg tcgatataca tcaccaactt
     6541 ccagttacag aaggtggatt acaaccatct cggaaccgat attttcgacc cgaagcctta
     6601 aaaacaaaaa tccgccccga gaggcggatt ttttatatca ccaaagtgat tagaagcggt
     6661 aaccaacacc ggcaatccag gtgcctacgt caacgctacg aatacggctc tgctcgtaag
     6721 agaagtccag agcaacgttt tccatcgggt tgaactgcag accagcaccg taggagaaac
     6781 cgtagtcgct ggtgtcgtgt ttgtaggtcg ggtattcagt ggtctggaat ttaccataac
     6841 ccacacccac tacaccgtag atgcttgccc agtcgttaat gcggtaagcc ggaccagcag
     6901 tgatgccgta gtactggttt ttgttgtagt caccagagct tgcagtacgg cttttctcgg
     6961 tgtaagtgaa agaaccgatc acacccagcg ggctgttgtc ttcttcatag cggtatttca
     7021 ggttgaaacc gcccattttg ttcatttggc cctgagcgtc gctctgtgcg taaccgccag
     7081 ttacggtaga agtcgcagct acggaagtac ctgcggtgaa agccagaact gcggccagtg
     7141 ctgaaagaca tgcaattttt ttcataacca cctcaaatgt gattcaaata agtcctaagt
     7201 tttaaatata tcaaaaatta atgggaaact cttcgcgatt tgtgatgtct aacgggccat
     7261 ttcatgtaac agaacgtttc catacaccgc tatccatcta aatttaaatc actttttcag
     7321 agaactgcgt aagtattacg catgttttcc ctgtcattca tccagattat tcctaatcac
     7381 cagactaatg attccatcaa tcctggcgca ttttagtcaa aacgggggaa aattttttca
     7441 acaaatgctc aaccagcatt gggtatatcc agtacactcc acgctttact taagtctaga
     7501 tatttgtggg agaaaggatg cctggttcat tacgtaaaat gccggtctgg ttaccaataa
     7561 tcatattgct cgttgccatg gcgtctattc agggtggagc ctcgttagct aagtcacttt
     7621 ttcctctggt gggcgcaccg ggtgtcactg cgctgcgtct ggcattagga acgctgatcc
     7681 tcatcgcgtt ctttaagcca tggcgactgc gctttgccaa agagcaacgg ttaccgctgt
     7741 tgttttacgg cgtttcgctg ggtgggatga attatctttt ttatctttct attcagacag
     7801 taccgctggg tattgcggtg gcgctggagt tcaccggacc actggcggtg gcgctgttct
     7861 cttctcgtcg cccggtagat ttcgtctggg ttgtgctggc ggttcttggt ctgtggttcc
     7921 tgctaccgct ggggcaagac gtttcccatg tcgatttaac cggctgtgcg ctggcactgg
     7981 gggccggggc ttgttgggct atttacattt taagtgggca acgcgcagga gcggaacatg
     8041 gccctgcgac ggtggcaatt ggttcgttga ttgcagcgtt aattttcgtg ccaattggag
     8101 cgcttcaggc tggtgaagca ctctggcact ggtcggttat tccattgggt ctggctgtcg
     8161 ctattctctc gaccgctctg ccttattcgc tggaaatgat tgccctcacc cgtttgccaa
     8221 cacggacatt tggtacgctg atgagcatgg aaccggcgct ggctgccgtt tccgggatga
     8281 ttttcctcgg agaaacactg acacccatac agctgctggc gctcggcgct atcatcgccg
     8341 cttcaatggg gtctacgctg acagtacgca aagagagcaa aataaaagaa ttagacatta
     8401 attaaattta catttctgca tggttatgca taaccatgca gaatttctcg ctacttttcc
     8461 tctacaccgt ctttatatat cgaattatgc aaaagcatat ttattccgaa aattcctggc
     8521 gagcagataa ataagaattg ttcttatcaa tatatctaac tcattgaatc tttattagtt
     8581 ttgtttttca cgcttgttac cactattagt gtgataggaa cagccagaat agcggaacac
     8641 atagccggtg ctatacttag tctcgttaat tactgggaca taacatcaag aggatatgaa
     8701 attatgagta ccgctaaatt agttaaatca aaagcgacca atctgcttta tacccgcaac
     8761 gatgtctccg acagcgagaa aaaagcaaca gtagagttgc tgaatcgcca ggttatccag
     8821 tttattgatc tttctttgat taccaaacaa gcgcactgga acatgcgcgg cgctaacttc
     8881 attgccgtac atgaaatgct ggatggcttc cgcaccgcac tgatcgatca tctggatacc
     8941 atggcagaac gtgcagtgca gctgggcggt gtagctctgg ggaccactca agttatcaac
     9001 agcaaaaccc cgctgaaaag ttacccgctg gacatccaca acgttcagga tcacctgaaa
     9061 gaactggctg accgttacgc aatcgtcgct aatgacgtac gcaaagcgat tggcgaagcg
     9121 aaagatgacg acaccgcaga tatcctgacc gccgcgtctc gcgacctgga taaattcctg
     9181 tggtttatcg agtctaacat cgaataaatc catcgctgat ggtgcagaac tttagtaccc
     9241 gataaaagcg gcttcctgac aggaggccgt tttgttttgc agcccacctc aacgcactta
     9301 tttagtgcat ccatctgcta tctccagctg attaagtaaa ttttttgtat ccacatcatc
     9361 acacaatcgt tacataaaga ttgttttttc atcaggtttt acgctaaata atcactgtgt
     9421 tgagtgcaca attttagcgc accagattgg tgccccagaa tggtgcatct tcagggtatt
     9481 gccctataaa tcgtgcatca cgtttttgcc gcatctcgaa aaatcaagga gttgcaaaac
     9541 tggcacgatt ttttcatata tgtgaatgtc acgcagggga tcgtcccgtg gatagaaaaa
     9601 aggaaatgct atgaagtctg tattaaaagt ttcactggct gcactgaccc tggcttttgc
     9661 ggtttcttct catgccgcgg ataaaaaatt agttgtcgcg acggataccg ccttcgttcc
     9721 gtttgaattt aaacagggcg ataaatatgt gggctttgac gttgatctgt gggcggccat
     9781 cgctaaagag ctgaagctgg attacgaact gaagccgatg gatttcagtg ggatcattcc
     9841 ggcactgcaa accaaaaacg tcgatctggc gctggcgggc attaccatca ccgacgagcg
     9901 taaaaaagcg atcgatttct ctgacggcta ctacaaaagc ggcctgttag tgatggtgaa
     9961 agctaacaat aacgatgtga aaagcgtgaa agatctcgac gggaaagtgg ttgctgtgaa
    10021 gagcggtact ggctccgttg attacgcgaa agcaaacatc aaaactaaag atctgcgtca
    10081 gttcccgaac atcgataacg cctatatgga actgggcacc aaccgcgcag acgccgttct
    10141 gcacgatacg ccaaacattc tgtacttcat caaaaccgcc ggtaacggtc agttcaaagc
    10201 ggtaggtgac tctctggaag cgcagcaata cggtattgcg ttcccgaaag gtagcgacga
    10261 gctgcgtgac aaagtcaacg gcgcgttgaa aaccctgcgc gagaacggaa cttacaacga
    10321 aatctacaaa aaatggttcg gtactgaacc gaaataataa cgctacacct gtaaaacgca
    10381 ctggcagttc cctctcccct ctggggagag gattagggtg aggggcgcaa acccgctccg
    10441 gggccattaa ttaccctgaa tttgattatt tacaccacgg taacaggaac aacatatgca
    10501 gtttgactgg agtgccatct ggcctgccat tccgcttctg attgaaggtg ccaaaatgac
    10561 cctgtggatt tcggtcctcg gtctggcagg cggtctggta atcggattgc tggcaggttt
    10621 tgcacgcacc ttcggtggtt ggatagccaa ccacgtcgcg ctggtcttta ttgaagtgat
    10681 ccgcggcaca cctatcgtcg tccaggtgat gtttatctat ttcgccctgc cgatggcgtt
    10741 taacgactta cgcatcgacc catttactgc ggcggtggtc accatcatga tcaactccgg
    10801 cgcgtacatt gcggaaatca cgcgtggtgc ggtgctgtct atccacaaag gttttcgtga
    10861 agcaggactg gcgctcggtc tttcacgttg ggaaaccatt cgctacgtca ttttaccgct
    10921 ggcactgcgt cgtatgctgc cgccgctggg taaccagtgg atcatcagca ttaaagacac
    10981 ctcgctgttt attgtgatcg gcgtggcgga actgacccgt caggggcaag aaattattgc
    11041 cggtaacttc cgcgcccttg agatctggag cgccgtggcg gtgttctatc tgattattac
    11101 cctggtgctg agctttattc tgcgtcgtct ggaaagaagg atgaaaatcc tgtgattgaa
    11161 tttaaaaacg tctccaagca ctttggccca acccaggtgc tgcacaatat cgatttgaac
    11221 attgcccagg gcgaagtcgt ggtgattatc gggccgtccg gttccggtaa atcgaccctg
    11281 ctgcgctgca tcaacaaact ggaagaaatc acctccggcg atctgattgt cgatggcctg
    11341 aaggttaacg atccgaaagt tgacgagcgc ctgattcgcc aggaagcagg tatggtgttc
    11401 cagcagtttt acctcttccc gcatctgacg gcgctggaaa acgtcatgtt tggtccgcta
    11461 cgcgtgcgtg gcgcgaacaa agaagaggcg gaaaaactgg cgcgtgagct gctggcgaaa
    11521 gtcggtctgg cagaacgtgc acatcactac ccttccgaac tttctggtgg tcaacagcag
    11581 cgtgtggcga ttgcccgcgc gctggcggtg aagccgaaaa tgatgctgtt tgatgaaccg
    11641 acttccgctc ttgacccgga actgcgccat gaagtgctga aggttatgca ggatctggct
    11701 gaagaaggga tgacgatggt gatcgtgacc cacgaaatcg gttttgccga gaaagtagct
    11761 tcgcggctga tctttatcga caaaggccgg attgcggaag atggcgatcc gcaggtattg
    11821 atcaagaacc cgccgagcca gcgcttgcag gaatttttgc agcacgtctc ttaacttctc
    11881 tgcctccctt tcacccgaaa gggaggcaca ccagattcct ctcatttaaa atcgcccctc
    11941 ccccagcatc tatacttatc tttttgctct gttttctcac tggaggagtc atgcggtgga
    12001 tcctgttcat cctcttctgc ctgctgggcg cacctgccca cgcggtatcc atacccggcg
    12061 ttacaaccac aacgacaacg gactcaacga ctgaaccggc cccggaaccg gatatcgaac
    12121 aaaaaaaagc ggcctatggc gcactggcgg atgtgctgga taacgacacc tcgcgtaaag
    12181 aattgatcga ccagttgcgt actgtcgccg cgacgccccc tgcggaaccg gtcccgaaga
    12241 tcgtaccgcc gacgctggtt gaagagcaaa ccgtgctgca aaaggtcacc gaagtcagcc
    12301 gccattatgg tgaagccctt tccgcccgct tcgggcaact ttatcgcaat atcaccggct
    12361 ccccgcataa gccatttaat ccacaaacct tcagcaatgc gctgacccat ttttcaatgt
    12421 tagcggtatt agtgtttggt ttttactggc tgattcgcct gtgcgcactg ccgctgtatc
    12481 gcaaaatggg tcagtgggcg aggcaaaaaa atcgtgagcg cagcaactgg ttgcagcttc
    12541 cggcgatgat tatcggggcg tttattatcg acctgctgtt actggccctg acattatttg
    12601 tcggccaggt attaagcgac aacctgaatg caggcagtcg caccatcgct ttccaacaaa
    12661 gtttgtttct caatgccttt gccctgattg aatttttcaa agccgtacta cgcctgattt
    12721 tttgcccaaa cgtggctgaa ctgcgcccgt tcacgattca ggacgagagc gcccgttact
    12781 ggagtcgtcg cctgagctgg ttaagcagtt tgataggtta tggcctgatt gtggccgtgc
    12841 cgattatctc taatcaggtg aatgtacaga taggtgcgct ggcgaacgtc atcattatgc
    12901 tgtgcatgac cgtctgggcg ttgtacctga tctttcgcaa taaaaaagag attacccaac
    12961 atttgctcaa ctttgcggag cattcgctgg cctttttcag cctgtttatc cgcgcctttg
    13021 cgctggtgtg gcactggctg gcaagcgcct attttatcgt gctgtttttc ttttcgttgt
    13081 tcgatccggg caacagcctg aaatttatga tgggtgcaac ggtgcgcagc ctggcgatta
    13141 ttggtatcgc agcgtttgtt tccggtatgt tttcccgctg gctggcgaaa accatcaccc
    13201 tctcgccaca tactcagcgt aactatccgg agctacaaaa acggctgaat ggctggcttt
    13261 cggcggcgct gaaaacggcg cgtattctga ccgtctgcgt ggcggtaatg ttgctgttga
    13321 gcgcatgggg attgttcgat ttctggaact ggctgcaaaa cggcgcgggg cagaaaaccg
    13381 tagatatcct gatccgcatc gcactcattc ttttcttctc ggcggttggc tggacagtgc
    13441 tcgccagttt gatcgaaaac cggctggctt cggatattca tggccgcccg ctacccagcg
    13501 cccgtacgcg taccctgctg acgctgttcc gtaacgcgct ggcggtgatt atcagtacca
    13561 tcaccatcat gattgtgttg tcggaaatcg gcgtcaatat cgcgccattg ctggcaggtg
    13621 ctggggcatt aggtctggct atctcgtttg gttcgcaaac gctggtgaaa gatattatca
    13681 ccggggtatt tattcagttt gaaaacggca tgaacactgg agatttggtt actatcgggc
    13741 cgttgaccgg cactgtggaa cggatgtcga ttcgctccgt gggcgtgcgt caggataccg
    13801 gggcgtatca catcattcct tggtcttcga taaccacctt tgctaacttc gtccgcggca
    13861 ttggttcggt ggtggcaaac tacgatgttg atcgccatga agatgctgat aaagccaatc
    13921 aggcactgaa agatgcggta gcggaattga tggaaaacga agaaattcgc gggctggtta
    13981 ttggtgaacc aaactttgcc gggattgtcg gcttaagcaa taccgcgttt acactgcgtg
    14041 tttcgttcac cacgctgcca ctcaaacagt ggacggtccg ctttgccctc gacagccagg
    14101 tgaaaaagca tttcgacctg gcaggtgttc gcgcgccagt gcagacttat caggtgctgc
    14161 ctgctccggg cgcggccccg gctgaaccgc tgccgccggg ggagccaacg ctttaacgtt
    14221 ggcgattaac aaaacggcgg cgctgctcgt cgttcataaa ggtccaggca ataaagcgac
    14281 tctgcttttg cccctgggcc atctcttttt taaccacctt caccgcgccc acgtcagtca
    14341 gagcacgata caacggcggt aagttttcac cacgagaaac cagcgaggta aaccacatca
    14401 cctgcttcgc gaagcctttg ctctcttcaa tcatcttttt gataaaggcg acttcaccac
    14461 cttcacacca caactcctgt tgctgaccac caaagttcag tgcatcgtct ttgttcagcc
    14521 ccaggttacg gcgtttacgc tcactacctg cccgtgccgc agcggcggaa tcgtggaatg
    14581 gcgggttaca caaggtcgcg tcgtattgct cgtttttatg gatgatgccg ttaaaaatcg
    14641 ccccactctc tttttgccga cgcagacgaa tggcacggtt aagccccgga ttagcactga
    14701 tgatcgcctg cgcactactt aacgcctgac tgctggtttc actaccggta aaacgccagc
    14761 catattcatg tacgccaatc agcggataaa tacagttcgc accaacgccg atatccagaa
    14821 tgctggcatt agctggaatt gttccgcttg cttctgccag taaatcggca aggtgatgaa
    14881 tataatccgc ccggcccggt actggcgggc agagaaaacc gtccgggata tcccagttcg
    14941 ctacggcgta aaaatgggcc agcaatgcct tattgagcgc cttcaccgcc agcggattgg
    15001 caaagtctac gctttgctcc cctgcgggtg taagcgtgag gaattgcctg agttcaggat
    15061 tgacctgaca aagcgtggcg agatcgtagc ggctgtgatg acggttgcgc ggatgcaacc
    15121 ccggtttctg ggcggacata gcattctcct ttaagcagcg gcgtaagata cccgcataac
    15181 gcgacagggt aaatatctgg ctgcgtttat cattccccaa aaccttaaca tggctatggt
    15241 ttatggcaat tcatcataaa aatcagcatt atgcatgtca ttgatttgtc ttattttgta
    15301 aaaatatatc gttcaaaaaa taagcacatc tctgtcaatt ttttgcgtca ttttcatcgc
    15361 ccaggcgacc tacacttatc aggcactacc cgttgtattt atgaggatgt cattatgaaa
    15421 aagtgcctca cactactgat tgccaccgtc ctgagcggaa tctctctcac ggcttatgcc
    15481 gcgcaaccga tgagtaacct ggacagcggt cagctacggc ccgccggtac cgtttcggca
    15541 actggcgcat caaacctaag cgatctggag gataaactgg cagaaaaagc gcgcgagcaa
    15601 ggcgcgaaag gttatgtcat taattccgca ggcggaaatg accagatgtt cggtactgca
    15661 accatctaca aataacccca ccaacgtaac tgcattcgcc tctggatgca gttttcgcct
    15721 gttttgtaat caaaaaacat caaatatgaa cgcaatgtaa ataaatgtat ttctttttcg
    15781 cgcaatgggt gatagaaaat cgcgccaagt gataatgctt atcaaaatta ttatcacttg
    15841 cacgaacact atcacgagat taacagtggc atcgcatccg cagagaggct ttctcgtggc
    15901 agtgaaaatt tcaacatata aaaaaaagtc acctgcaaaa tggaaaacaa tcgcaatttc
    15961 cctgccagac aatttcattc gctcacgttc tttgccggtc tttgtattgg catcacgcct
    16021 gtggctcagg cactcgccgc cgaagggcaa actaacgcgg atgacacgct ggttgtcgaa
    16081 gcatcaacgc cttcgcttta tgcgccacaa caatctgccg atccgaaatt ctcgcgtccg
    16141 gtagcggata ctacccgcac gatgacggtg atttctgaac aagtgattaa agatcagggc
    16201 gcaaccaacc ttaccgacgc gctcaaaaac gtccccggcg tgggtgcgtt ttttgcgggt
    16261 gagaacggta actccaccac tggcgacgcc atttatatgc gcggtgctga tacctctaac
    16321 agtatttata ttgatggcat tcgcgatatc ggcagcgtct cgcgcgacac cttcaatact
    16381 gagcaggtcg aagtgattaa agggccttcc ggcaccgact acgggcgcag cgcgccgacg
    16441 ggctcgatca atatgatcag caagcagccg cgcaatgatt ccggcattga cgcctccgcc
    16501 agtattggca gcgcctggtt ccgccgcggc acgctggacg tcaatcaggt cattggtgat
    16561 accactgcgg tgcgcctgaa tgtgatgggc gaaaaaacgc acgatgccgg acgcgacaaa
    16621 gtcaaaaatg agcgttacgg cgtcgcccct tctatcgctt ttggccttgg tacagcgaat
    16681 cgtttgtatc ttaattatct gcatgtcacc cagcacaaca cgccagacgg cggcattccg
    16741 accatcggtt tgccgggtta ttctgcccca tctgcgggaa cagcggctct gaatcattcc
    16801 ggaaaagttg atactcataa cttttacggc acggattccg attacgacga ttcgaccacc
    16861 gacaccgcca ccatgcgttt tgagcacgac atcaacgata acaccaccat tcgcaatact
    16921 acccgttggt cgcgcgtaaa gcaggattac ctgatgacgg cgattatggg cggggcgtcg
    16981 aatattactc agcccaccag cgatgtgaat agctggacct ggtcacgcac ggcgaatacc
    17041 aaagatgtga gtaataaaat tctcaccaac cagaccaacc tgacctcgac gttctatacc
    17101 ggttctatcg gtcatgatgt cagtaccggc gtggaattta cccgtgaaac gcagactaac
    17161 tacggcgtta atccggtgac gttacccgcg gtaaatattt atcatcctga cagcagcatt
    17221 catcccggcg gcctgacgcg caacggcgca aacgccaatg gtcagacgga taccttcgca
    17281 atttacgcct tcgatacgct gcaaatcacc cgtgattttg agctgaacgg cgggatccgt
    17341 ctggataatt atcatactga atatgacagt gccaccgcct gcggcggcag cggacgcggt
    17401 gccatcacct gcccagctgg tgtggcaaaa ggttctccgg tcaccaccgt cgacaccgcc
    17461 aagtcgggca atctggtgaa ctggaaagcc ggggcgctgt atcacctgac ggaaaacggt
    17521 aatgtctata ttaactatgc cgtttcccag cagcctccgg gcggcaacaa cttcgccctt
    17581 gcgcagtctg gcagcggtaa cagtgccaac cgcaccgatt ttaaaccgca aaaagccaac
    17641 accagcgaga ttggcaccaa atggcaggtt ctggataaac gcctgttgct caccgccgcg
    17701 ctattccgta ctgatatcga aaatgaagtt gagcaaaatg atgacggaac ttactcgcaa
    17761 tacggtaaga aacgtgtcga aggctatgag atatccgtgg ccgggaatat cacgcccgcg
    17821 tggcaggtga ttggcggcta tacccagcaa aaagcaacca tcaaaaacgg caaagatgtt
    17881 gcccaggatg gttcctcatc gctgccgtat accccggagc acgccttcac cttatggagc
    17941 cagtatcagg caacagatga tatctctgtt ggcgcgggcg cacgctatat cggcagtatg
    18001 cataaaggtt cagacggcgc ggtgggaacg ccagcgttta ccgaaggtta ctgggtcgcc
    18061 gatgccaaac tggggtatcg ggttaatcgc aatctcgact tccagctaaa cgtttacaac
    18121 ctgtttgata ccgattacgt cgcctcaatc aacaagagcg gctaccgtta tcacccgggc
    18181 gagccaagaa ccttcttgct cacagccaat atgcatttct gattcagatg tggggcgcag
    18241 gccccacttt ttggagaact tgtatgatgt accacattcc cggcgtgtta tcgccacagg
    18301 acgtcgcccg gtttcgcgaa caactggaac aagccgaatg ggtggatgga cgcgtcacca
    18361 ccggcgcaca aggtgcgcaa gttaagaaca atcaacaggt cgacacccgc agcacgttat
    18421 acgccgccct gcaaaatgag gtgctgaacg cggttaacca acatgcttta ttctttgccg
    18481 cggccttgcc gcgtaccctt tccacgccgc tgtttaatcg ctatcagaac aatgaaacct
    18541 atggtttcca tgtggatggc gcagtacgca gccatccaca aaacggctgg atgcgtactg
    18601 acctttctgc cacgctgttt ttaagcgatc cacaaagcta cgacggcggc gaactggtcg
    18661 ttaatgacac cttcggacaa catcgggtaa aactcccggc aggcgatctc gtgttgtatc
    18721 cctccagcag cctgcattgc gtgacacccg taacccgcgg cgtacgagtg gcatcattta
    18781 tgtggatcca gtcgatgatc cgtgatgata aaaagcgcgc catgctgttt gaactggaca
    18841 acaatattca gtcgctgaaa agccgctatg gtgaaaatga agagatcctg tcgctgctta
    18901 atctttatca taatctgctg cgggaatggt cggagatctg atgcttgaac tgcccaagtt
    18961 cgtccctgga ttagctacac ttaactgtac aagcattgat atggggagga cgatatggca
    19021 tccggttggg ctaacgatga cgccgtcaac gaacagatca acagtacaat tgaagatgcg
    19081 attgcccgcg ctcggggtga aattccgcgt ggcgaaagcc tggatgaatg tgaagagtgc
    19141 ggtgccccca tcccgcaagc ccgtcgggaa gccattcctg gcgtgcgctt atgcattcat
    19201 tgtcagcagg agaaagattt acaaaaacca gcgtatacag gatataatcg cagaggttcg
    19261 aaagacagcc agttacgtta actacaactg gcgagatgta tagcgagtat aatttgtatt
    19321 ttgcgtggca ttccgtgcct ttaacgctgt cgtgactgcc acgctgcata aaacaagtcc
    19381 ataagtacaa caaataaatg gtttatcagt atgttagata tcaatcaatt tatttgaaca
    19441 aggcggtcaa ttctcttcga ttttatctct cgtaaaaaaa cgtgatactc atcacatcga
    19501 cgaaacaacg tcacttatac aaaaatcacc tgcgagagat taattatgaa aactatcaat
    19561 actgttgttg ctgctatggc tctttcaact ctgtcatttg gcgtgtttgc cgcggaaccg
    19621 gtaacggcat cccaggcaca gaacatgaat aaaatcggcg tggtttctgc cgatggcgca
    19681 tccaccctcg atgccctgga agcgaaactg gctgagaaag ccgcggcagc cggtgccagt
    19741 ggatacagca tcacttccgc caccaacaac aataaattaa gcggtactgc ggtaatttat
    19801 aagtaattcg taggaaggat aaggcgttta cgccgcatcc ggcaacagat gcctgatgcg
    19861 acgctaccgc gtcttatcag gcctacaaaa tccgaaccgt tggacggatc aggcgtttac
    19921 gctacgtcca gcacaagtat ttccctgtct gtttgccgac agacgcatat gctctaaccc
    19981 tcattgatcc tatgttaccc ttgtttgccc gtccgccact ggacgggctt ttttttagct
    20041 ggctaactgc tgacagaaag cctgcaacgt ctcttccggc ataccaatct gccgcgctgc
    20101 atcacaaatg gcctgccagc ttcccgcatc cagtggaatc ccctgcttct gccgttcgcg
    20161 acgcgtgttc acttcccact cgcccggtag caaaatcggc ttatcatcat catgcggcga
    20221 ggctttcacc cactcggcaa aggcttcggt ctgcgcgtta caatccggcg cgccgaacag
    20281 ttccgggttg atgatgatag tggtcatgca gttaagaatg gcatcgggac tggtttgtaa
    20341 cgtttcctga tgcgtcgttt taccgccgga aagcgccccg ccaagaattt cacacatcgc
    20401 tgcaagggcg tagcctttat gttcggcaaa ggtcaacagc gaacccaacg gcgactcctg
    20461 cattaccgcc ggattggtcg tcggcacgcc gttaacgtca atcaggcaac ctggcggcac
    20521 ggggacgcct ttatgccagg cgacgcgggt tttgccaaat gcaatggcgc tggtggcgta
    20581 atcaagcaac agcgggaaat tatctttacg agggaaaacc acacagaacg gattggtgcc
    20641 aaagcggctg tcgcgaccgt ggaacggcgc gaccattgga ataccgacca cgctaacaaa
    20701 gtggatagag acaaaccccg ccgctgcaca ctgctccgcc cagtaaccga tacggccgat
    20761 atgatgcgag ttatgtagcg ccacggcggc aataccgtgc tgatgcgctt tctcaatccc
    20821 cagcgccatc gcttcatgtg ccgcgacctg accaaatgcg cgatcgccat cgagcgtgac
    20881 cgccgccccc gcctctttaa cggttttggc atgatggtta atttgcaggt gcccctgact
    20941 ccaggagcgt acatagcttg ggatcatgcc aataccatgt gaatcatgcc ctgccaggtt
    21001 tgccgcgatt aaatgatcgg caactaattt cgcttcttgt tcctcgctac ccatctgacg
    21061 aaatacagcc tgaataaaac tgtgcagcgt ctgagcatca aagcgatgac cactttccat
    21121 ggttaactcc tgtttatgtt atgtgtttgt tgtattttta tgctgcacgc cgggcgtggg
    21181 aaggtcaata aagatgcagg aatttcatat tgcggtgggg taaaagccgg atgacatggc
    21241 tcatccggct gagaaaagaa ttaaaatgcc tgattaacgc gcgccaggcc gtcgctgata
    21301 gttgccgctt cacccgtagc caccaggcag caagccatct ggattttcag cgattcggga
    21361 atcggttcgc tgccagcaag gcaacgctca atccactgcg ccgtggtttc cggatctttt
    21421 gcttgtggca gtaactcgct accagcagtg tcctgttttt catacagcac ccgcatccct
    21481 tcacggtcaa tgagattgat ctgcgggcag cgctgcggat tagcatacac ttcaccttca
    21541 gtaccatgca ttagtagcgc ccgcccgccg atatcgctaa agaacttcgc gacgcgtcca
    21601 atgtattccg gatgcgaaac gctggaaaga cgcagcgcct caccttcggc aaatggcgtc
    21661 gccagtttcg ccagggtatg tgcactgtta cgcacgccca tccgccagcg catcgccagt
    21721 tgtttttcca gcggcgggca aaacgcgccg actggcataa acaccggttg atgttcgtcg
    21781 agcttcgcct gcgcctgccc gccgtgcaac gttggcgtaa tacccatcaa ttcaaaaatg
    21841 gtttcagtca gcacgcgggt tggatcttcg ctaaccccgt gaaccaccac aggaaaaccg
    21901 agtttgtgca ggagaatcgc caacaacggc gtcaggttgg cctgtttacg cgcgccgttg
    21961 taactgggga tgacaatcgg catcggcttg cctgctggcg gcgtcagctt gatggtgtga
    22021 ttttgcatgg cttcgtaaaa gccgagcatc tctgcttccc cttctccttt gatacgcagc
    22081 gcaatcaata cgccgcccaa ctcaaggtca gggacttcac cattgagcat atgagcatac
    22141 agaccgcgcg cagtatcccg gtctaaatcg cgcgcgtggt ttttcccgcg cccgatctct
    22201 ttaatgattt tgcgatagtc catttacgac tccttacctg actcacatca ttaacgccgc
    22261 cgacggcgtg gggatttcgt tttttctttc tttttaacta taccttcagg gacttccggt
    22321 tgctctatcg gaaataccgg taatgcatcc agtagtcgct tgccataatt tttggtcagc
    22381 aagcgtttgt cgtagataac cacttcgccc cagcaaccgt ggcttcgaat cagtcgccca
    22441 acctgctgaa tcaggttaaa cgaggcgctc ggcaggcttt gcacctcaaa cggatagcgg
    22501 ttgaggcttt tcagccattc cccttcggtg atcaccaccg ggctgtcgat gggcggaaaa
    22561 gcgattttgt ggatatgcac ctggctgagc agatcacctt tcaaatcaag cccttcggca
    22621 aatgactgta agcccaccag cacgctgcgc tcaccgttgg cgacgcgttt gcggtgcagt
    22681 tcaactaaac ggtaacgcgg ctgatcgccc tgaaccagca acatcagacg taaatccgtc
    22741 acatagtcga gaaagcgctg catcgcccgt ccgctggcaa acagtaccaa cataccgaga
    22801 tgttttttgc tctccacctg cttacggaaa aaggccgcca tttcggcaat atgctgctct
    22861 tcgttgtcga tggaaggctc aacgcgcatc cggggaataa caattttgcc ctgttcgcag
    22921 tggttaaagg gggaatccag cgccacaaaa cggtcgcccg ctttctcttt cagaccactc
    22981 atctcctgca aacgcgaaaa actgttcagc gaacgcaagg ttgcggaggt gacaataatg
    23041 tgcggaatac tgcgccacag cagcctttcc agctgatcgc tgacacgtat tcccacgcag
    23101 tgaaaccaga ggtgtagctg cccttcgcgc tcttcccgcg tcgcccattt ggtcaccggt
    23161 gcaccggaag attgcgccag cgaagccagc cgccagagtt tgctttgcgc ctcgaacatc
    23221 cccaacgcgc ggttcatctg caaaatcaac cgatgcagac gtacaatgtc atggctgccg
    23281 gttttctcac ttaaatcgtt aagaaataac tccgccaggc cacgcagcat ctcggtgagt
    23341 tttgccagcc gctggcagat ctccagcact tcatctggca gttcgcccat cgcaaaacgg
    23401 tgctctgcct cctgcccggc aggcatgtag agattgagaa tgttgtttaa cgaggcgata
    23461 agctcataca actcttcaca atgcgcattc aaacgttcag ggatcgccag cggtgggatg
    23521 gtcttcgggc gaaactgctc catgcaggta gcgaccagtt tcgtgaacaa gtccagctgt
    23581 agccgatacc acggcgcggt gatttcggcg ctcatctcaa gcgcatcccg cgccacatcc
    23641 ggcaggtgat ggccttcgtc cagcaccagc agtaaatttt tcgggtcagg caataccgct
    23701 tcgctttcca tcgccgccat caccagcgca tggtttgcca ccaccacttc cgcttcctga
    23761 atctcccgac gagcaacaaa aaacgggcat tcacgatagt agtaacagtt acggttgagg
    23821 cagctggctt tgtcggtact caaacgacgc cagagatcgt catttatagc gatatccgta
    23881 tgatcgcgca gaccatccca tttataagta tcgagatcgc ccttcagctt cgcacaacgt
    23941 ttttgctctt cctgattgtt cggcgtcagt tcgtcgtcaa gaaacgccag cagatcctgt
    24001 tgcgtgggtt cagtactggc gagcgccgtc agattacgcg gacaaacgta gcgcccacgc
    24061 ccaaaagcgg cagtgaattt aagatcggga atgatctttt tcagcagcgg taaatctttg
    24121 ctgtaaatct gatcctgcaa tgccacgttg gcggtactca ccaccagcgt tttttgctct
    24181 tcgcgggcaa tggcgatgcc gggaatcaaa taagagagcg ttttcccaac gccggtgggg
    24241 gcttcaatcg ccagatgtcg cccttcttct ccggccagcg ttttggcgac gtccgcaatc
    24301 atctgccgct gcggcgcacg gggaataaag tcggggatct gttcctgaag cgccttatac
    24361 caggcggcaa tttgcgcttt aagcgcggcg gttaatgcca tgagaaaacc tgaaatactg
    24421 tataaacagc caatattgtg gcatttttcg gttttcaggg gtaatggttt ttctgtttct
    24481 ggaaagcggc tcgcaataac caccaacata atgcggttta atgcccgttc gagcacaaag
    24541 gatccaacac gtcatgcaag acaccatcat aaatttctac agcaccagcg atgactacgg
    24601 tgatttttct aactttgccg cctggcccat caaggttgac gggaaaacct ggcccacctc
    24661 agaacactat tttcaggcac aaaaattcct tgatgaaaaa taccgcgaag agatccgtcg
    24721 ggtttcttcc cctatggtcg ccgcacgcat ggggcgcgat cgttctaagc ctctgcgtaa
    24781 aaactgggag tcggtcaaag aacaggtgat gcgaaaagca cttcgcgcta aattcgaaca
    24841 gcatccagaa ctgcgtgcgc tcttgctggc aaccgcgccc gcaaaactgg ttgagcatac
    24901 ggaaaacgat gcttactggg gagacggtgg taatggtaag ggcaagaatc gactgggcta
    24961 ccttttaatg gagttgcgcg aacaattggc tatagagaag taacgtctcg gtggcttatg
    25021 ggcggtaaaa cagataaatt ttttcccgtg ttctcaaatt gctgacaagt gcttgttgtg
    25081 aaatgccgga tgcggcgtaa acgccttatc cggcctacaa aagcatgcaa attcaatata
    25141 ttgcagagtt cacgtaggcc tgataagcgt agcgcatcag gcagttttgc gtttgttcat
    25201 cagcctgatg ccgggcatag cccggcataa aagattactg cgcagcggta ggtttacgcg
    25261 gacggcggcg acgttgttgt tcgcctgccg gtttggcatc gccgaggcgg cgagacggtt
    25321 tttctgcagg tttcgcgctt gcagattttg cgccaccttc cccacggcgt ggttgctgtt
    25381 gaccgcgacc accaccttgc ccacgaccgc cgccgccacg ttgctggcga ccgttctgga
    25441 tcggttcggc tttgattgac gggtccggct catagcccgg aatcgcaatg cgcgggatct
    25501 cttttttcag cagtttttcg atatcacgca gcagtttgtg ttcatcaaca cacaccagcg
    25561 acaacgcttc accggtagca gccgcacgac cggtacgccc gatacggtgg acataatctt
    25621 ccggtacgtt tggcagttca tagttgacga cgtgcggcaa ctcttcgata tccagaccgc
    25681 gcgcagcgat atcggtcgcc accagtacgc gaatgtcacc cgatttaaaa tcagccagcg
    25741 cacgggtacg cgcaccttgc gatttattcc cgtggatcgc cgcactacgg atgccatctt
    25801 tattgagctg ctcagccaga tggttagcgc cgtgtttggt acgggtaaac accagcacct
    25861 gctgccagtt ccctttacca atcatgtgcg acagcaattc gcgtttgcgt ttcttatcga
    25921 caaagtgaac gtgctgggtc acctgatcag acgcggtatt gcggcgtgcc acttcgattt
    25981 ccagcgggtt gtgcaacagt ttttccgcca gggctttaat atcgtcagag aaggtcgcag
    26041 agaacagcag gttctggcgt ttcgctggca gttttgttaa cacgcgacgg atatcgtgga
    26101 taaagcccat gtcgagcatg cggtcagctt catcgaggac gaggatttca acctgatcca
    26161 gcttcactgc attttgatgt tccaggtcca gcaaacgtcc cggtgttgcc accagaacat
    26221 caacgccgcc gcgcagcttc atcatttgcg ggttaatgct gacaccgcca aacaccacca
    26281 gcgaacgaat gttcaaatat ttgctgtaat cacggacgtt ttcgccaatc tgcgcagcca
    26341 gttcacgggt cggggtaaga atgagcgcac gtaccggacg acgccctttg gcgtgcggct
    26401 ggcgagtgat caggtgttgc aacagcggca gcgtaaagcc cgctgtttta ccggtaccgg
    26461 tctgggcgct agccatcagg tcgcggcctt ccagcaccgc agggatcgcc tgctgctgaa
    26521 taggggtggg ttcacggtaa ccctgctcgg caacggcgcg caggatatca gggcttaaac
    26581 ccaaagaatc gaaagacata actactccga accgccccga ccgttaccgg tgtagttttc
    26641 agggagatac tgaaaaggaa aatgacaaaa accacaatgt catgagggag cggagtgtag
    26701 cagtttttgt gacgcagcgc ataaattatc cctgtgactg gcgtagctaa aattcttagc
    26761 catactggac aactccccta tggagtcata atcttaatca atcatttgat taagaatgga
    26821 ctgtgcgatg aataatcctg ccatgacaat caagggtgaa caggcgaaaa aacagctgat
    26881 tgctgccgca ctggcgcagt ttggtgaata tggaatgaac gccaccaccc gcgagatagc
    26941 cgcccaggcc gggcagaata tcgctgccat cacctactac ttcggttcga aagaagattt
    27001 gtacctcgcc tgcgcccagt ggattgccga ttttattggc gagcagttcc gtccgcacgc
    27061 cgaagaggcc gaacgcttgt tcgcacaacc acagcctgat cgcgcagcca tccgtgaact
    27121 gatccttcgc gcctgcagga acatgattaa gctgctcacc caggatgaca ccgtcaacct
    27181 cagcaagttt atctcccgtg agcagctctc tcccacggca gcctatcacc tggtgcacga
    27241 acaggtgatt agcccgctac acagccacct gacacgcctg attgccgcct ggaccggctg
    27301 cgacgccaac gatacccgca tgatcctcca tactcatgcg ttgattggtg agattctggc
    27361 gttccgtctt ggcaaagaaa caattctgtt acgtaccggc tggaccgcgt tcgatgaaga
    27421 aaagaccgaa ctgatcaacc agacggtgac ctgtcatatc gacctgattt tgcaaggatt
    27481 atcgcaaagg agtttgtagt gatgaaaaaa cctgtcgtga tcggattggc ggtagtggta
    27541 cttgccgccg tggttgccgg aggctactgg tggtatcaaa gccgccagga taacggcctg
    27601 acgctgtatg gcaacgtgga tattcgtacg gtaaatctta gtttccgtgt tggggggcgc
    27661 gttgaatcgc tggcggtgga cgaaggtgat gctatcaaag cgggccaggt gctgggcgaa
    27721 ctggatcaca agccgtatga gattgccctg atgcaggcga aagcgggcgt ctcggtggca
    27781 caggcgcagt atgatctgat gcttgccggg tatcgcgatg aagaaatcgc tcaggccgcc
    27841 gcagcggtga aacaggctca agccgcctat gactatgcgc agaacttcta taaccgccag
    27901 caaggactgt ggaaaagccg caccatttcg gcaaatgacc tggaaaatgc ccgctcctcg
    27961 cgcgaccagg cacaggcaac gctgaaatca gcacaggata aattgcgtca gtaccgttcc
    28021 ggtaaccgtg aacaggacat cgctcaggcg aaagccagcc tcgaacaggc gcaggcgcaa
    28081 ctggcgcagg cggagctgaa tttacaggac tcaacgttga tagcaccgtc tgatggcacg
    28141 ctgttaacgc gcgcggtgga gccaggcacg gttctcaatg aaggtggcac ggtgtttacc
    28201 gtttcactaa cgcgtccggt gtgggtacgt gcctatgttg atgaacggaa tcttgaccag
    28261 gcccagccgg ggcgcaaagt gctgctttat accgatggtc gcccggacaa gccctatcac
    28321 ggacagattg gtttcgtttc gccgactgct gaatttaccc cgaaaaccgt cgaaacaccg
    28381 gatctgcgta ccgacctcgt ctatcgcctg cgtattgtgg tgaccgacgc cgatgatgcg
    28441 ttacgccagg gaatgccggt gacggtgcaa ttcggtaacg aggcaggaca tgaatgatgc
    28501 cgttatcacg ctgaacggcc tggaaaaacg ctttccgggc atggacaagc ccgccgtcgc
    28561 gccgctcgat tgtaccattc acgccggtta tgtgacgggg ctggtggggc cggacggtgc
    28621 aggtaaaacc acgctaatgc ggatgctggc gggattactg aaacccgaca gcggcaatgc
    28681 cacggtgatt ggctttgatc caatcaaaaa cgacggcgcg ctgcacgccg tgctcggcta
    28741 tatgccgcag aaatttggtc tgtatgaaga tctcacggtg atggagaacc tcaatctgta
    28801 cgcggatttg cgcagcgtca ccggcgaggc acgtaagcaa acttttgctc gcctgctgga
    28861 gtttacgtct cttgggccgt ttaccggacg cctggcgggc aagctctccg gtgggatgaa
    28921 acaaaaactc ggtctggcct gtaccctggt gggcgaaccg aaagtgttgc tgctcgatga
    28981 acccggcgtc ggcgttgacc ctatctcacg gcgcgaactg tggcagatgg tgcatgagct
    29041 ggcgggcgaa gggatgttaa tcctctggag tacctcgtat ctcgacgaag ccgagcagtg
    29101 ccgtgacgtg ttactgatga acgaaggcga gttgctgtat cagggagaac caaaagccct
    29161 gacacaaacc atggccggac gcagctttct gatgaccagt ccacacgagg gcaaccgcaa
    29221 actgttgcaa cgcgccttga aactgccgca ggtcagcgac ggcatgattc aggggaaatc
    29281 ggtacgtctg atcctcaaaa aagaggccac accagacgat attcgccatg ccgaagggat
    29341 gccggaaatc aacatcaacg aaactacgcc gcgttttgaa gatgcgttta ttgatttgct
    29401 gggcggtgcc ggaacctcgg aatcgccgct gggtgcaata ttgcatacgg tagaaggcac
    29461 acccggcgag acggtgatcg aagcgaaaga actaaccaag aaatttggtg attttgccgc
    29521 caccgatcac gtcaactttg ccgttaaacg aggggagatt tttggtctgc tcgggccaaa
    29581 cggcgcgggg aaatcgacca cctttaagat gatgtgcggc ttgctggtgc cgacctccgg
    29641 tcaggcgctg gtgctgggga tggatctgaa agagagttcc ggtaaagcgc gccagcatct
    29701 cggctatatg gcgcaaaagt tttcgctcta tggcaacctg acggtcgaac agaatttacg
    29761 ctttttctct ggtgtgtatg gcttacgcgg tcgggcgcag aacgaaaaaa tctcccgtat
    29821 gagcgaggcg ttcggcctga aaagtattgc ctcacacgcc accgatgaac tgccattagg
    29881 ttttaaacag cggctggcgc tggcctgttc gctgatgcat gaaccggata ttctgtttct
    29941 cgacgaaccg acttccggca ttgaccccct cacccgccgt gaattttggc tgcacatcaa
    30001 cagcatggta gagaaaggcg tcacggtgat ggtcaccacc cactttatgg atgaagcgga
    30061 atattgcgac cgcatcggcc tggtgtaccg cgggaaatta atcgccagcg gcacgccgga
    30121 cgatttgaaa gcacagtcgg ctaacgatga gcaacccgat cccaccatgg agcaagcctt
    30181 tattcagttg atccacgact gggataagga gcatagcaat gagtaacccg atcctttcct
    30241 ggcgtcgcgt gcgggcgctg tgcgtcaaag agacgcggca gatcgttcgc gatccgagta
    30301 gttggctgat tgcggtagtg atcccgctgt tgctgctgtt tatttttggt tacggcatta
    30361 acctcgactc cagcaagctg cgggtcggga ttttactgga acagcgtagc gaagcggcgc
    30421 tggatttcac ccacaccatg accggttcgc cctacatcga cgccaccatc agcgataacc
    30481 gtcaggaact gatcgccaaa atgcaggcgg ggaaaattcg cggtctggtg gttattccgg
    30541 tggattttgc ggaacagatg gagcgcgcca acgccaccgc accgattcag gtgatcaccg
    30601 acggcagtga gccgaatacc gctaactttg tacaggggta tgtcgaaggg atctggcaga
    30661 tctggcaaat gcagcgagcg gaggacaacg ggcagacttt tgaaccgctt attgatgtac
    30721 aaacccgcta ctggtttaac ccggcggcga ttagccagca cttcattatc cccggtgcgg
    30781 tgaccattat catgacggtc atcggcgcga ttctcacctc gctggtggtg gcgcgagaat
    30841 gggaacgcgg caccatggag gctctgctct ctacggagat tacccgcacg gaactgctgc
    30901 tgtgtaagct gatcccttat tactttctcg ggatgctggc gatgttgctg tgtatgctgg
    30961 tgtcagtgtt tattctcggc gtgccgtatc gcgggtcgct gctgattctg ttttttatct
    31021 ccagcctgtt tttactcagt accctgggga tggggctgct gatttccacg attacccgca
    31081 accagttcaa tgccgctcag gtcgccctga acgccgcttt tctgccgtcg attatgcttt
    31141 ccggctttat ttttcagatc gacagtatgc ccgcggtgat ccgcgcggtg acgtacatta
    31201 ttcccgctcg ttatttcgtc agcaccctgc aaagcctgtt cctcgccggg aatattccag
    31261 tggtgctggt ggtaaacgtg ctgtttttga tcgcttcggc ggtgatgttt atcggcctga
    31321 cgtggctgaa aaccaaacgt cggctggatt agggagaaga gcatgtttca tcgcttatgg
    31381 acgttaatcc gcaaagagtt gcagtcgctg ctgcgcgaac cgcaaacccg cgcgattctg
    31441 attttacctg tgcttataca ggtgattctg ttcccgttcg ccgccacgct ggaagtgact
    31501 aacgccacca tcgccatcta cgatgaagat aacggcgagc attcggtgga gctgacccaa
    31561 cgttttgccc gcgccagcgc ctttactcat gtgctgctgc tgaaaagccc acaggagatc
    31621 cgcccaacca tcgacacaca aaaggcgtta ctactggtgc gtttcccggc tgacttctcg
    31681 cgcaaactgg ataccttcca gaccgcgccg ttgcagttga tcctcgacgg gcgtaactcc
    31741 aacagtgcgc aaattgccgc caactacctg caacagatcg tcaaaaatta tcagcaggag
    31801 ctgctggaag gaaaaccgaa acctaacaac agcgagctgg tggtacgcaa ctggtataac
    31861 ccgaatcttg actacaaatg gtttgtggtg ccgtcgctga tcgccatgat caccactatc
    31921 ggcgtaatga tcgtcacttc actttccgtc gcccgcgaac gtgaacaagg tacgctcgat
    31981 cagctactgg tttcgccgct caccacctgg cagatcttca tcggcaaagc cgtaccggcg
    32041 ttaattgtcg ccaccttcca ggccaccatt gtgctggcga ttggtatctg ggcgtatcaa
    32101 atccccttcg ccggatcgct ggcgctgttc tactttacga tggtgattta cggtttatcg
    32161 ctggtgggat tcggtctgtt gatttcatca ctctgctcga cacaacagca ggcgtttatc
    32221 ggcgtgtttg tttttatgat gcccgccatt ctcctttccg gttacgtttc tccggtggaa
    32281 aacatgccag tatggctgca aaacctgacg tggattaacc ctattcgcca ctttacggac
    32341 attaccaagc agatttattt gaaggatgcg agtctggata ttgtgtggaa tagtttgtgg
    32401 ccgctactgg tgataacggc cacgacaggg tcagcggcgt acgcgatgtt tagacgtaag
    32461 gtgatgtaac ttcttatctt tcgccagcaa agacactacc gccgggccgg caaggattgc
    32521 cagccctgca attgccagca aaaagttgtt ttccagtaca cgggacaaca gccacagcac
    32581 aatcagcgct gcgccaaaat accaggtcgt ggtcagctct tccagcacgt ctgccacctc
    32641 acgccagcgt tgctcggggt ggcgctgaaa caccaacatc atcactaccg tgacacaata
    32701 cagtgcgcat aatcccacgc cgacgtgaac cagagtctta ctcatccagc ccaccaccag
    32761 cagcgccact accagtaaat gcaacataat ctgccagcaa cttagaccag ttgcgacacg
    32821 aacacgttgt tgccacttca tggcttctct cctgaagaat ttatctctac ttactcagag
    32881 taccgaactt tacggaaaat tccgtaagac cgcacctttt tgtgacgccg actacactat
    32941 ttcaatgaga taagaggcaa agacaggaaa attatgcccg atcaaacaca acaattttcg
    33001 ttcaaggtgc tcaccatcaa cattcacaaa ggttttaccg cgtttaaccg acgcttcatt
    33061 ttgccggaac ttcgcgacgc ggtgcgtacc gtcagcgccg atattgtttg cctgcaggaa
    33121 gtgatgggcg cgcacgaagt tcatccgctg catgtggaaa actggcccga tacctcgcac
    33181 tacgagtttc tcgccgacac gatgtggagc gattttgcct acggtcgcaa tgccgtatac
    33241 ccggaagggc atcacggcaa cgccgtactg tcgcgttatc ccattgaaca ttatgagaat
    33301 cgcgatgttt cggtcgatgg tgcggaaaag cgcggcgtgc tctactgccg cattgtgcca
    33361 ccgatgaccg gaaaagcgat tcatgtgatg tgcgtacatc tgggcctgcg tgaggcgcac
    33421 cgtcaggcgc agcttgcgat gctcgccgaa tgggtgaatg agctaccgga cggcgaaccg
    33481 gtattggtgg cgggtgattt caatgactgg cggcaaaaag ctaatcatcc gttaaaagtg
    33541 caggccggac tggatgagat ttttacccgc gtccacggac gcccggcgcg cacgtttccg
    33601 gtgcaatttc ctctactacg actggacagg atctacgtca aaaatgccag cgccagcgcg
    33661 ccaaccgcgt tgccgctgcg gacatggcga cacctttctg atcatgcccc tttaagtgcg
    33721 gagattcatt tatgaaatgt agctggcgcg aaggcaataa gatccagttg ctggaaaacg
    33781 gcgagcaata ttatcccgcg gtgtttaagg cgattggcga ggcacaagaa cgcatcattc
    33841 ttgaaacgtt tatctggttt gaggatgacg tcggcaaaca gctgcatgcg gcactactgg
    33901 cagcagcgca acgcggggtt aaagcggaag tcttgctgga tggctacggt tcgccggatc
    33961 tcagcgatga gtttgtcaat gaactgacgg cagctggcgt ggtatttcgc tactacgatc
    34021 cccgccctcg cctttttggt atgcgcacca atgtgtttcg ccggatgcat cgcaaaattg
    34081 tggtgatcga cgcgcgtatc gcctttattg gcgggctgaa ttactccgcc gagcatatgt
    34141 ccagctacgg tccagaggct aaacaggatt acgcggtacg ccttgaaggg ccgattgtcg
    34201 aagatattct ccagtttgag ctggaaaacc tgcctggaca gagcgcggca cgacgctggt
    34261 ggcgacgtca tcacaaagcg gaagagaacc gccagccggg agaagcgcag gtattgctgg
    34321 tctggcgcga taacgaagaa catcgcgatg atattgaacg tcactatctg aaaatgctca
    34381 ctcaggcgcg gcgagaagtc attatcgcca acgcctactt cttccccggc tatcgatttt
    34441 tacacgcctt gcgtaaagcg gcacggcgcg gggtgcggat caaactgatc attcagggcg
    34501 aaccggatat gccgattgtc agagtcggtg cgcgcttgct gtataactat ctggttaaag
    34561 gcggcgttca ggtatttgag taccgccgcc gcccgctcca cggcaaagtg gcattgatgg
    34621 acgatcactg ggcgacagta gggtccagta atctcgatcc gctcagtttg tcactgaatc
    34681 tcgaagcaaa tgtcatcatc cacgatcgtc attttaacca gacgctgcgc gataatctga
    34741 acggcattat tgccgcagat tgtcagcagg tcgatgagac catgctgccg aaacgcacct
    34801 ggtggaacct gaccaaaagc gtgctggcgt tccacttttt acgccacttc ccggcgctgg
    34861 ttggctggct tccggcacac acaccacgtc tggcgcaggt tgatccgccc gcacaaccga
    34921 caatggaaac gcaggatcgg gtagaaactg aaaacacggg ggtaaaaccc tgatgagtaa
    34981 atcacacccg cgctggcgct tagcaaagaa gatcctcacc tggctgtttt ttatcgcggt
    35041 gattgtgttg ctggtggtct acgccaaaaa agtggactgg gaagaggtct ggaaggtcat
    35101 ccgcgactac aatcgcgttg cgctgcttag tgcggtcggg ctggtggtcg tcagctatct
    35161 gatttacggc tgctatgacc tgctcgcccg cttttactgc ggtcacaaac tggcgaagcg
    35221 ccaggtgatg ctggtgtcgt ttatctgcta cgccttcaac ctgacgctca gtacctgggt
    35281 cggcggcatt ggtatgcgct atcgtttgta ctcccgacta gggttaccgg gcagcactat
    35341 tacgcggatt ttctcgctca gtattaccac caactggctg ggctatattt tactggcagg
    35401 gattatcttt accgcaggcg tggtggagtt gccggatcac tggtatgtcg atcaaactac
    35461 gctgcgtatt ctcggcattg gcttactgat gattatcgcg gtttatttgt ggttttgcgc
    35521 tttcgcgaag caccgccata tgaccatcaa agggcaaaag ctggtgctgc cttcatggaa
    35581 attcgccctc gctcagatgc tgatttccag cgttaactgg atggtaatgg gggcgattat
    35641 ctggctgtta cttggtcaaa gcgtgaacta tttctttgta ctgggcgtgt tactggttag
    35701 tagtattgct ggcgtcatcg tgcatattcc ggcggggatc ggtgtgctgg aagcggtgtt
    35761 tatcgcacta ctggctgggg agcatacctc gaaagggagc attatcgccg ccctgctcgc
    35821 ttatcgcgtc ctgtattact ttatcccgct gctgctggcg ctgatctgtt acctgctact
    35881 ggaaagccag gcgaagaagc tacgggcgaa aaatgaagcg gcgatgtgaa gtttagttaa
    35941 gttctttagt atctgcattt acgactaatg aaaaaaacgc gtatgccttt gccagacaag
    36001 cgttatagct atcgcggcaa gtatcccaaa gaagccgata aaatagtaat agagtgttaa
    36061 tgcacctgta acagcgcaac gaaccggact ccacaactct tcactatggc atttccccgc
    36121 caattcaagg agcttatttc gtccatgcga gataatgcct gaccataaca caatcattaa
    36181 gcaacttatt acccaaacgg gtcgttcact cattaatgcg gcattcatga tgatcaccag
    36241 ggccaaccct gtcaacgtca tcataatgat aaaacggtga gaaccaggat caacattaaa
    36301 taataaaccg gccagcatac tgattaaaaa catcgcgccc gcagtgctga acacgacgat
    36361 aatggcaggt acaggagcta aacttgcaac gacaatcgcc accaaagaga gtaaaaacag
    36421 atatattacg cctgcgatga cggcatttat cttgttgata tccttactaa gccatacata
    36481 caaactaata ataaccctat ggcgacatac attgttggtg aatcatattt ggttgccgta
    36541 atttcgagat acttagctcc ccaggccagt cctgcggttg ccaccaaacc taaagcggta
    36601 atgagccata taccgtttaa tatcctggtc tcgtgctgaa aatctaattt attgctgttt
    36661 tgtgagtatg attccattct tttagttgtc cttagaaaaa atattaatat tcaccatcaa
    36721 taaatttaaa accacgcata caaaatactt ttgtatttat ggatacggaa aacgcttccg
    36781 ctcgccataa ggcgtatttt atacctggta cttgttaagt gtcgttttta gtaagaaaaa
    36841 aaatcccgac acatggccgg gatattgatg gtgaaaaaga attaacggcg gttgccaaaa
    36901 attcgcagca acatcaggaa caggttgatg aagtccagat acaaggttaa cgcgccaaga
    36961 atggaatatt tgcgcaggtt cgacgtgtcg cgggtatcaa tctgctcacc catatttttc
    37021 aatttctgag tgtcatacgc cgtcaatccg acaaagacaa tcacgccgat gtaggtaact
    37081 gcccacatca atgcttcgct tttcagccag aagttgacca gcgatgccag cacaatgccg
    37141 attaacgcca taaacagcat attgccaaag ccacttaaat cgcgcttcgt ggtgtaaccg
    37201 tacaggctca ttgcgccgaa catcccggca gtaacgacga aagtactggc gatagaagca
    37261 gcggtataga caatgaatat actggaaagc gtaagacccg tcagcgccga ataaagcata
    37321 aagagcatcg tcgttacacc tgcgctcagc ttttgaatca tcgctgataa cacaataacc
    37381 aatgctaatt gcgcgatgat cagaccgatt aaaaagacac ggttagtaaa caacagctcc
    37441 atcacggccg cggaattagc cgcataccag gcaacaaatg cggtcagcaa caagccaacg
    37501 gtcatccagc catagacttg cgccatataa gtttgcaagc cagcccgggg ttgtacgatt
    37561 gaatcagaac gtgggaatct gtccatgacg atctcctgaa gatataagga atatcttaag
    37621 gatactgcaa aatgatgagg ctgtgcatcg acgcagcgta aacgcatgta ctgagcggtg
    37681 aaattgccgg acgcagcggt gccttatccg gctaacaaaa aactaccagc gttttgccgc
    37741 ctgctgatcg ctctcccggg cttcaaccca gcggtcgcct tccggcgtgg cttcgcgctt
    37801 ccagaacggt gcgcgggttt tgagataatc cataataaac tgcccggctt caaacgcact
    37861 gctgcgatgc gcactggtga caccgacaaa aacgatttca tcgcccggcc ataattcccc
    37921 gatgcggtga atcaccgtga cgcgccccag cggccagcgg ttacgcgctt catcaacaat
    37981 ttctgccagt gctttttcag tcatccccgg atagtgttcg agggttaatg ctttgacgct
    38041 gtcgcccagg ttatggttgc gcaccttacc ggtaaaggtg actaccgcac cgtcttcgtc
    38101 acgctccgcc agccacgggt actcttctcc tacgctgaac ggctgcgggc cgacaacaat
    38161 tttggtttct gccatcttaa cctccggtta ccggcgggaa gaaagctact tcgtcgccgt
    38221 cagtcagcga atggtcaaaa ctcaccagcg tctggttaac ggcagccagc aatttgccat
    38281 cctccagcgc caacgcccag cgatcgctct gcgcagccag gtgctggcgt aacgcttcaa
    38341 cggttgggaa atccgcagcc acttcggttg catctgttcc caccaactcg cgcacctggg
    38401 cgaaaaaaag aactttaatc atccgcttcc accttaaagt cacccgactt gccgccgctc
    38461 ttcgccagca aacgtaccgg accaatcacc atatcttttt gcaccgcttt gcacatgtca
    38521 taaatggtca gcgccgccac ggaggccgcg gttaatgctt ccatttcgac accggttttc
    38581 ccggtcaggc ggcataaggt ttctatacgc acccgattgt gctccggctc ggcctgtaaa
    38641 ttgacttcaa ctttgctgag catcagcgga tgacagagcg ggatcagatc ccaggtgcgt
    38701 tttgccgcct gaataccggc aatacgcgca gtggcaaata cgtcgccttt gtggtggcga
    38761 ccatcaataa tcatcgccag cgtctcgctg cgcatggtga caaaggcttc cgcccgcgct
    38821 tcacgcacgg tttccgcttt ggcggagaca tccaccatgt gcgcttcgcc agcggcgttg
    38881 atatgggtca gttgcgacat acttatttct tcaaatgtgg atggaaatta cacggacgcg
    38941 tacgggcatc cagctgcggc gcgatgatat tttcccatgc ggtacggcac gctttggtcg
    39001 aacccggcat ggcgaaaatc agcgttttgt tggcgacgcc cgctaccgca cgagattgca
    39061 acgtggaagt gccaatctct tcaaacgaca acatacggaa cacttcacca aaaccttcaa
    39121 tttcacggtc gaacaacggc agcaatgctt cgggagcctg atcaccttcc gtcaggccag
    39181 taccacccgt aatcaatacc acttgtacat cgtcgctggc gatccacgca gatacctgag
    39241 cgcgaatagc gtagcggttt tctttcacaa tggctttatc gacaacgtga tggcccgctt
    39301 cttgcgccga atcgcgcaga tagtgaccgg aggtatcgtc ttcttcaccg cgacgattag
    39361 aaaccgtaag aatagcaata cgggtcggga taaattcagt gcttacctga ctcatctgat
    39421 ctctcctttt gacgttttag ccgccaatgt acgataagtt ttgcgtaata ccggtgttgt
    39481 tttgatgcag gaaatgggtc tgtttcttct cccgcagcgc cgctgaaata cgcgcttcca
    39541 gcgcctgttg ctgggtatcg tcttccagca gatcgcgcag gttaacgccg ccttcaccaa
    39601 acaggcagag atggagttta ccaatggagg aaacgcgcag gcggttgcaa gtggcgcaga
    39661 agtctttttc atacggcatg ataaggccaa tctctccggc gtaatctgga tggcaaaaga
    39721 cttgcgcggg accgtcgctg cgttgacgta attggtggat ccagccgcga cgcagtagct
    39781 cgtcacgcag aacctgacca gagatgtgat gcttacggaa gagctcgctg ccctcgcccg
    39841 tttccatcag ttcgatgaaa cgcagctgga tagggcgatg ctggatccag ttcagaaagg
    39901 tgtcgagctg gtgatgatta acatcacgca tcagcacggt attgactttg accttctcaa
    39961 aaccggcctc aaatgcagca tcaatccccg ccatgacctg gttgaattta tcctgcccgg
    40021 taatagcgtg aaactggcgg gcgtccagac tgtcgacact gacgttaatg ccagtaagtc
    40081 ccgcatcgcg ccagttcgcc acatcgcgtt ccagacggta accattggtg gtgaccgcaa
    40141 tctggcggat agcgtcgttt tcccgcacag cggcgatgat atcggtaaag tcgcggcgta
    40201 aagacggctc accgcctgtc aggcgcactt tttcggtgcc cagactggcg aaggcgcgcg
    40261 taacccggcg aatttcatcg acggtaagaa agcctttatt ggtgacgccg ctcggtttgt
    40321 agccatccgg caggcagtag gtgcaacgaa agttacacac atcggtaatc gacaggcgca
    40381 agtagtaaaa cttacgcgca aatgcatcag tcagttgtga agccatgtac acctttccag
    40441 atacgggagg cgaagtcatt tcttccttcg ccctggtggc aatctttacg agcattgtca
    40501 cggccaaagc accgtatcag ttgacccagg tgcagaggct agagtgttta gtggttatgc
    40561 cgatactagc gtgtaaatga gatttttacc atccacactt tcgctatgta atcatgtata
    40621 tagcgtcatg atcgtgctat ttcgctacag aataagggaa aatggcggtt ttactttgat
    40681 atacatcatc agggaggcat agcgtgcatc gtcctttaga ggtagaagaa aatagtttgt
    40741 atcctcaata ttggcaggtt aattgctgtt tccccgcaat ttgcgctact gtagcgcgga
    40801 taatctgact ccaggaaact atatgcgcaa tcgtacgctg gctgatcttg atcgtgtcgt
    40861 tgctctcggc ggagggcatg gactgggacg cgttctctca tcactttcgt ctttgggttc
    40921 tcgtttaacg ggtatcgtca ccaccaccga taatggtggc tcgacggggc gtattcgccg
    40981 ttcagaaggc ggcattgcct ggggcgatat gcgcaactgc ctcaaccagc tgataacgga
    41041 accgagcgtc gcctccgcga tgtttgaata ccgttttggt ggcaatggcg aactttccgg
    41101 tcataatctc ggaaacttga tgttaaaggc gctggatcac cttagcgtgc ggcctctgga
    41161 agccatcaat ttaattcgta atctgctgaa agtggatacg catttgattc caatgtcaga
    41221 gcatcctgtt gatctgatgg cgattgacga tcaggggcat gaagtttacg gcgaggtcaa
    41281 tatcgaccag ttaactacgc cgattcaaga gttattgtta acgcctaatg tacccgcaac
    41341 gcgtgaggcg gttcacgcta tcaatgaagc ggatctcatc attattgggc ctggcagttt
    41401 ttataccagc ctgatgccaa ttctgctgct gaaggaaatc gcccaggcat tacgccgcac
    41461 gccagcgccg atggtttata tcggcaatct ggggcgtgag ttgagtttac ctgcggctaa
    41521 tttgaagctg gaaagcaagc tggcaattat ggagcagtat gttggtaaaa aggtcattga
    41581 tgcggtcatc gtcgggccaa aagtggatgt ctcggcggtg aaagagcgga ttgtgatcca
    41641 ggaggtactg gaggccagcg atattccgta tcgtcatgac cgccagttgt tacataacgc
    41701 gctggaaaag gcgttacagg ctttaggtta acggaaatcc gaaggaaaat tccggcttcc
    41761 tattgaagac aaagtgcgcg ttgtttatgc cggatgcggc gtgaacgcct tatccggcct
    41821 acaaaccgcg caaattcaat atattgcgga gaaaatgtag gcctgataag cgtagcgcat
    41881 caggctgttt tccgtttgtc atcagtcttc ttcgctatcc tgttacgatg ccgcgataaa
    41941 cagctcacgc agctgatgca actggtcacg aatttgcgcc gcttcttcga actccagatt
    42001 ctgcgcgtgt tgcatcatca acccttccag ctcatggatt ttctgctgca acgctttagg
    42061 cgacatatcc atcggcacat tatccggctc aacaatcggt cgcgattttc ctctgccctt
    42121 cgctttggtt ttagcaatgt tctgccccaa cgccaggata tcgaccactt tcttgttcaa
    42181 gccttgcggc gtaatgccgt gttcctcgtt gtacttctgc tgtttctcgc ggcgacgttc
    42241 ggtttcgcca atcgctttcg ccattgatgg ggtgatctta tcgccgtaga gaatcgcttt
    42301 accgttaacg ttacgtgccg cacgaccaat ggtctggatc aacgaacgtt cggaacgcag
    42361 gaagccttct ttgtcagcgt cgaggatcgc caccagcgaa acttccggca tatccagacc
    42421 ttcacgcagt aagttgatcc ctaccaatac gtcgaactca cccagacgca agtcgcggat
    42481 aatctccata cgttcgacgg tgtcgatatc tgagtgaaga taacgcacgc gctcgccatg
    42541 ttcttcgaga tattcggtaa gatcttccgc catccgcttg gtcagtgtgg tgaccagtac
    42601 gcgttcgtta attgccgctc gctgacgaat ctccgaaaga agatcatcaa cctgtgtcgc
    42661 caccggccgc acttcgataa tcgggtcaag caatccggtt ggacgcacca cctgatccac
    42721 cacatcgccg ccggattttt ccagctcgta attacccggc gtcgccgaaa catagatggt
    42781 ttgcggcgct aatgcttcga actcttcaaa cttaagcgga cggttatcca gcgctgatgg
    42841 caggcggaag ccgtactcca ccagtgtctc tttacgcgcc cggtcaccgc gatacatgcc
    42901 gccaatttgt ggaatggtga cgtgagattc atcgaccacc agcagcccat cagcagggag
    42961 gtaatcaaac agcgtcggcg gtggctcacc cggtccacga ccggagagga agcgcgagta
    43021 gttttcaatc cccgaacagt agcccagctc gttcatcatc tccagatcaa actgggtacg
    43081 ctgggtcagc cgctgctctt ccagcagttt gttgttttcc aacagcactt tgcgtctggc
    43141 ggccagctct tctttgatct cctccatcgc ctgtacgatg cgctcgcgcg gtgtgacgta
    43201 gtgcgttttc gggtagatgg taaaacgtgg aatagtggaa acaatctgcc cggtcagcgg
    43261 gtcaaataac gacaatcgtt ccacttcctc gtcaaacagt tccacgcgaa gtgcaatgtc
    43321 atccgattct gccgggaaga tatctatcac ctcgccacga acgcggaaag taccacgctg
    43381 gaatgcttga tcattacgag cgtattgcag ctccgccagt cggcgcagaa tcgcgcgctg
    43441 atcgataatc ataccgaccg tgagatggag catcatcttg agatataaat caggatcgcc
    43501 cagaccataa atcgcggaaa cagacgccac cacaaccaca tcacgccgct ccagcatcgc
    43561 tttggtggcg gacaaacgca tctgctcaat atgttcgtta accgaggcat ctttctcaat
    43621 gaaagtgtcg gaactcggta cataggcttc cggctgatag tagtcgtagt aggaaacgaa
    43681 atattccacc gcgttttccg ggaagaactc tttcatttcg ccatacagct gggccgccag
    43741 cgttttgttg ggcgcaagta ccatggttgg gcgctgaagg tcagcaatga cattggcaat
    43801 ggtgaatgtt ttcccggagc cagtcacgcc aagtaacgtc tggtgcgcca ggccatcttc
    43861 cagcccctct tcgagacgtc gaatcgcctc tggctgatcg ccagaaggtt taaaagcgga
    43921 attcagtttg aacggtttac tcatgagtcg ctacctgaag gagttgggcg ggcaggtatg
    43981 taattttact cgtcgtactt aattatgcca acaaattata ctggataaaa aaacagttca
    44041 tcaccataat atttctgtta cagcgtaaac tccgctgtca atcatgagca aaatttactc
    44101 tgtggcgaga taaaactccg gccttaccgg gttatcccca aagcaacggc ttttttaaca
    44161 tttgtcaaga tgagtgatga cagttttatg gcaagaga
//
LOCUS       DAZFQD010000021        43759 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.021, whole genome shotgun sequence.
ACCESSION   DAZFQD010000021 DAZFQD010000000
VERSION     DAZFQD010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 43759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43759
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.021"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            142..726
                     /gene="yadN"
                     /locus_tag="ACHKTW_001276"
     CDS             142..726
                     /gene="yadN"
                     /locus_tag="ACHKTW_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO6462337.1"
                     /translation="MSKKLGFALSGLMLAMVAGTASADMDGGQLNISGLVVDNTCETR
                     VDGGNKDGLILLQTATVGEIDAGVLNDTVGAKAKPFSITVDCSKANPNPGSTAKMTFG
                     SVFFGNSKGTLNNDMSINNPSDGVNIALHNIDGSTIKQVQINNPGDVYTKALDATTKS
                     AVYDFKASYVRAVADQTATAGYVKTNTAYTITYQ"
     gene            831..1571
                     /gene="yadV"
                     /locus_tag="ACHKTW_001277"
     CDS             831..1571
                     /gene="yadV"
                     /locus_tag="ACHKTW_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HFO6462338.1"
                     /translation="MFFNTKHTTALCFVTCMAFSSSSIADIVISGTRVIYKSDQKSVN
                     VRLENKGNNPLLVQSWLDTGDDNAEPGSITVPFTATPPVSRIDAKRGQTIKLMYTAST
                     SLPKDRESVFWFNVLEVPPKPDAEKVANQSLLQLAFRTRIKLFYRPDGLKGNPSEAPL
                     ALKWFWSGSEGKASLRVTNPTPYYVSFSSGDLEASGKRYPIDVKMIAPFSDEVMKVTG
                     LNGKASSAKVHFYAINDFGGAIEGNASL"
     gene            1606..4206
                     /gene="htrE"
                     /locus_tag="ACHKTW_001278"
     CDS             1606..4206
                     /gene="htrE"
                     /locus_tag="ACHKTW_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Yad fimbria usher protein HtrE"
                     /protein_id="HFO6462339.1"
                     /translation="MTIKPTKNYHNHLTRIATFCALLYCNSAFCAELVEYDHTFLMGQ
                     NASNIDLSRYSEGNPAIPGMYDVSVYVNDQPIINQSITFIEIEGKKNAQACITLKNLL
                     QFHINSPDINNEKAVLLARDETLGNCLNLTEIIPQASVRYDVNEQRLDIDVPQAWVMK
                     NYQNYVDPSLWENGINAAMLSYNLNGYHSETPGRRNDSIYAAFNGGMNLGAWRLRASG
                     NYNWMTDSGSNYDFKNRYIQRDIASLRSQLILGESYTTGETFDSVSIRGIRLYSDSRM
                     LPPTLASFAPIIHGVANTNAKVTITQGGYKIYETTVPPGAFVIDDLSPSGYGSDLIVT
                     VEESDGSKRTFSQPFSSVVQMLRPGVGRWDISGGQVLKDDIQDEPNLFQASYYYGLNN
                     YLTGYTGIQITDNNYTAGLLGLGLNTSVGAFSFDVTHSNVRIPDDKTYQGQSYRVSWN
                     KLFEETSTSLNIAAYRYSTQNYLGLNDALTLIDEVKHPEQDLEPKSMRNYSRMKNQVT
                     VSINQPLKFEKKDYGSFYLSGSWSDYWASGQNRSNYSIGYSNSASWGSYSVSAQRSWN
                     EDGDTDDSVYLSFTIPIEKLLGTEQRTSGFQSIDTQMSSDFKGNNQLNVSSSGYSDNA
                     RVSYSVNTGYTMNKASKDLSYVGGYASYESPWGSLAGSVSANSDNSRQVSLSTDGGFV
                     LHSGGLTFSNDSFSDSDTLAVVQAPGAQGARINYGNSTIDRWGYGVTSALSPYHENRI
                     ALDINDLENDVELKSTSAVAVPRQGSVVFADFETVQGQSAIMNITRSDGKNIPFAADI
                     YDEKGNVIGNVGQGGQAFVRGIEQQGNISIKWLEESKPVSCLAHYQQSSEAEKIAQSI
                     ILNGIRCQIQ"
     gene            4223..4792
                     /gene="yadM"
                     /locus_tag="ACHKTW_001279"
     CDS             4223..4792
                     /gene="yadM"
                     /locus_tag="ACHKTW_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO6462340.1"
                     /translation="MIKITPHKIAILMGILLSPSVFATDINVEFTATVKATTCNITLT
                     GNNVTNDGNNNYTLRIPKMGLDKIANKTTESQADFKLVASGCSSGISWIDTTLTGNAS
                     SSSPKLIIPQSGDSSSTTSNIGMGFKKRTTDDATFLKPNSAEKIRWSTDEMQPDKGLE
                     MTVALRETDAGQGVPGNFRALATFNFIYQ"
     gene            4807..5409
                     /gene="yadL"
                     /locus_tag="ACHKTW_001280"
     CDS             4807..5409
                     /gene="yadL"
                     /locus_tag="ACHKTW_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein YadL"
                     /protein_id="HFO6462341.1"
                     /translation="MTFKNLRYGLSSSVVLAASLFSVPSYAATDSIGLTVITTVEIGT
                     CTATLVNDSDQDISVVDFGDVYISEINAKTKVKTFKLKFKDCAGIPNKKAQIKLTKRA
                     TCEGTANDGAGFANGSTAADKASAVAVEVWSTETPATGSATQFSCVTPASQEVTISNA
                     ANAVVYYPMSARLVVEKNKTVSNVTAGKFSAPATFTVTYN"
     gene            5436..6032
                     /locus_tag="ACHKTW_001281"
     CDS             5436..6032
                     /locus_tag="ACHKTW_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HFO6462342.1"
                     /translation="MHPTQRKLMKRIILFLSLQFPITYPAIAGQDIDLVANVKNSTCK
                     SGISNQGNIDLGVVGVGYFSDNVTPESYQPGGKEFTVTVSDCALQGTGDVLNQLHIDF
                     RALSGVMAAGSRQIFANEVATGAKNVGVVLFSIQDPTNIFNVISSAGNSRSVYPVMTS
                     ALHNSSWKFYARMQKIDPALDVISGQVMSHILVDVYYE"
     gene            6016..7359
                     /locus_tag="ACHKTW_001282"
     CDS             6016..7359
                     /locus_tag="ACHKTW_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001462196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like adhesin"
                     /protein_id="HFO6462343.1"
                     /translation="MCTTNNRSKQITATVTAVDGGVIMKKFFRHFLFLILCLSCYTAS
                     AGTDDNVGYIVGNSYGVGPSDQKWRETGPNGDATVIFRYATSTNNLVFYKPTQLGPTG
                     VKLQWSQLDTASGGGFLYCNRSDSTSGSAMRIENAMVDSGKMYGSHKLFNTSVPGLYY
                     TLLISNMWSAYGTVTNVSSPGIYIGDSAEQYFSWYNPSEDVLYWSCNNANSTRKYWAV
                     GGIYQTLTIEFYTDTNFDPTVTQQIKLSSSSNYLYSFKAYGAGQGINEHSYFIKIDFD
                     LLNVKLTNPTCFTAMLSGTSVTGSTVKMGEYSAEQIRNGATPVPFDISLQNCVRVTNI
                     ETKLVSTKVGTENGQLLGNTLTGNDAAKGVGVLIEGLATSKNPLMTLKPNDSNSVYKD
                     YDPRGKDDTTGGVYPDQDTGITYPLHFQATLQQDGTIPIEAGEFKATSTFQVTYP"
     gene            7472..8266
                     /gene="panB"
                     /locus_tag="ACHKTW_001283"
     CDS             7472..8266
                     /gene="panB"
                     /locus_tag="ACHKTW_001283"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HFO6462344.1"
                     /translation="MKPTTIASLQKCKQDKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVEDIAYHTAAVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGDEAG
                     DQLLSDALALEAAGAQLLVLECVPVELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            8278..9129
                     /gene="panC"
                     /locus_tag="ACHKTW_001284"
     CDS             8278..9129
                     /gene="panC"
                     /locus_tag="ACHKTW_001284"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HFO6462345.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHDGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIITIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKMVELA"
     gene            complement(9203..10104)
                     /gene="rpnC"
                     /locus_tag="ACHKTW_001285"
                     /pseudo
     CDS             complement(9203..10104)
                     /gene="rpnC"
                     /locus_tag="ACHKTW_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414674.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
     gene            10378..10758
                     /gene="panD"
                     /locus_tag="ACHKTW_001286"
     CDS             10378..10758
                     /gene="panD"
                     /locus_tag="ACHKTW_001286"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HFO6462346.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(10762..11991)
                     /gene="yadE"
                     /locus_tag="ACHKTW_001287"
     CDS             complement(10762..11991)
                     /gene="yadE"
                     /locus_tag="ACHKTW_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFO6462347.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAIWAQIGDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLITWKDTPVYNAPSAGSAPFGVLADNLRYPILHKLKDRLNQTWYQIRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMAQ
                     LEGYVKNKINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATAFVVTSRIKRHPQKW
                     NPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLETMSRLVSNQPQG"
     gene            complement(12055..12495)
                     /gene="yadI"
                     /locus_tag="ACHKTW_001288"
     CDS             complement(12055..12495)
                     /gene="yadI"
                     /locus_tag="ACHKTW_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFO6462348.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRERIVELGGPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(12600..13370)
                     /gene="yadH"
                     /locus_tag="ACHKTW_001289"
     CDS             complement(12600..13370)
                     /gene="yadH"
                     /locus_tag="ACHKTW_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO6462349.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(13367..14293)
                     /gene="yadG"
                     /locus_tag="ACHKTW_001290"
     CDS             complement(13367..14293)
                     /gene="yadG"
                     /locus_tag="ACHKTW_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO6462350.1"
                     /translation="MTIALELQQLKKTYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            14402..15064
                     /gene="can"
                     /locus_tag="ACHKTW_001291"
     CDS             14402..15064
                     /gene="can"
                     /locus_tag="ACHKTW_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HFO6462351.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(15105..15641)
                     /gene="hpt"
                     /locus_tag="ACHKTW_001292"
     CDS             complement(15105..15641)
                     /gene="hpt"
                     /locus_tag="ACHKTW_001292"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFO6462352.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            15847..18237
                     /gene="gcd"
                     /locus_tag="ACHKTW_001293"
     CDS             15847..18237
                     /gene="gcd"
                     /locus_tag="ACHKTW_001293"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HFO6462353.1"
                     /translation="MAINNTGSRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGGILTWAGFNDPQEINGTLSANATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQINADNVHNLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPVIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPKKDLSGADMWGATMFDQLVCRVMFHQL
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLIPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(18284..19834)
                     /gene="cueO"
                     /locus_tag="ACHKTW_001294"
     CDS             complement(18284..19834)
                     /gene="cueO"
                     /locus_tag="ACHKTW_001294"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HFO6462354.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGEKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLTEETTLHW
                     HGLEVPGEVDGGPQGIILPGGKRSVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFNADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVSELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGT
                     QFRILSENGKPPAAHRAGWKDTVKVEGNVSEVLVKFNHDAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            20000..20347
                     /gene="yacC"
                     /locus_tag="ACHKTW_001295"
     CDS             20000..20347
                     /gene="yacC"
                     /locus_tag="ACHKTW_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HFO6462355.1"
                     /translation="MKTFFRTVLFGSLMAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLPNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            20453..21319
                     /gene="speE"
                     /locus_tag="ACHKTW_001296"
     CDS             20453..21319
                     /gene="speE"
                     /locus_tag="ACHKTW_001296"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HFO6462356.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFKLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQPS"
     gene            21335..22129
                     /gene="speD"
                     /locus_tag="ACHKTW_001297"
     CDS             21335..22129
                     /gene="speD"
                     /locus_tag="ACHKTW_001297"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HFO6462357.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(22167..22529)
                     /gene="yacL"
                     /locus_tag="ACHKTW_001298"
     CDS             complement(22167..22529)
                     /gene="yacL"
                     /locus_tag="ACHKTW_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YacL"
                     /protein_id="HFO6462358.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDGEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(22705..25302)
                     /gene="acnB"
                     /locus_tag="ACHKTW_001299"
     CDS             complement(22705..25302)
                     /gene="acnB"
                     /locus_tag="ACHKTW_001299"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HFO6462359.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVDVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLNSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLADIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            25657..27414
                     /gene="yacH"
                     /locus_tag="ACHKTW_001300"
     CDS             25657..27414
                     /gene="yacH"
                     /locus_tag="ACHKTW_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HFO6462360.1"
                     /translation="MKMTLPFKPHVLALICSAGLCAASTGLYIKSRTVEAPVEPQSTQ
                     QTAPDITAVTLPATVSAPPVTPAVVKSAFSTAQIDQWVAPVALYPDSLLSQVLMASTY
                     PANVAQAVQWSHDNPLKQGDAAIQAVSDQPWDASVKSLVAFPQLMALMGENPQWVQNL
                     GDAFLAQPQDVMDSVQRLRQLAQQTGSLKSSTEQKVITTTKKAVPVTQTVTAPVIPSN
                     TVSTASPVITEPATTVISIEPANPDVVYIPNYNPTVVYGNWANTAYPPVYLPPPAGEP
                     FIDSFVRGFGYSMGVATTYALFSSIDWDDDDHDHHHHDDDDYHHHDGGHRDGNGWQHN
                     GDNINIDVNNFNRITGEHLTDKNMAWRHNPNYRNGVPYHDQDMAKRFHQTDVNGGMSA
                     TQLPAPTRDSQRQAAASQFQQRTHAAPVITRDTQRQAAAQRFNEAEHYGSYDDFRDFS
                     RRQPLTQQQKDAARQRYQSASPEQRQAVREKMQTNPQNQQRREAARERIQSATPEQRQ
                     VFKEKVQQRPLNQQQRDNARQRIQSASPEQRQVFREKVQESRPQRLNDSNHTVRLNNE
                     QRSAVRERLSERGARRQER"
     gene            complement(27485..28909)
                     /gene="lpdA"
                     /locus_tag="ACHKTW_001301"
     CDS             complement(27485..28909)
                     /gene="lpdA"
                     /locus_tag="ACHKTW_001301"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFO6462361.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(29234..31126)
                     /gene="aceF"
                     /locus_tag="ACHKTW_001302"
     CDS             complement(29234..31126)
                     /gene="aceF"
                     /locus_tag="ACHKTW_001302"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HFO6462362.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEAAPAAAPAPAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAAPAAKAEGKSEFAENDAYVHATPLIR
                     RLAREFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKV
                     DFSKFGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAK
                     RKLDVKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVV
                     PVFKDVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVN
                     APEVAILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSD
                     IRRLVM"
     gene            complement(31141..33804)
                     /gene="aceE"
                     /locus_tag="ACHKTW_001303"
     CDS             complement(31141..33804)
                     /gene="aceE"
                     /locus_tag="ACHKTW_001303"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HFO6462363.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKEISTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(33965..34729)
                     /gene="pdhR"
                     /locus_tag="ACHKTW_001304"
     CDS             complement(33965..34729)
                     /gene="pdhR"
                     /locus_tag="ACHKTW_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HFO6462364.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
     gene            35270..36643
                     /gene="aroP"
                     /locus_tag="ACHKTW_001305"
     CDS             35270..36643
                     /gene="aroP"
                     /locus_tag="ACHKTW_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HFO6462365.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAVVAMIIFGGWLLFSGNGGPQATVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTHFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(36686..37540)
                     /gene="ampE"
                     /locus_tag="ACHKTW_001306"
     CDS             complement(36686..37540)
                     /gene="ampE"
                     /locus_tag="ACHKTW_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HFO6462366.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPRAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(37537..38088)
                     /gene="ampD"
                     /locus_tag="ACHKTW_001307"
     CDS             complement(37537..38088)
                     /gene="ampD"
                     /locus_tag="ACHKTW_001307"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HFO6462367.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRPDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDCYPDIAKNMTGHCDIAPD
                     RKTDPGPAFDWARFRVLVSKETT"
     gene            38176..39069
                     /gene="nadC"
                     /locus_tag="ACHKTW_001308"
     CDS             38176..39069
                     /gene="nadC"
                     /locus_tag="ACHKTW_001308"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HFO6462368.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     SLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            39272..39712
                     /gene="ppdD"
                     /locus_tag="ACHKTW_001309"
     CDS             39272..39712
                     /gene="ppdD"
                     /locus_tag="ACHKTW_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HFO6462369.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWTRNCNIQSDSALHQACEDVFRFDDAN"
     gene            39722..41107
                     /gene="gspE"
                     /locus_tag="ACHKTW_001310"
     CDS             39722..41107
                     /gene="gspE"
                     /locus_tag="ACHKTW_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
                     /protein_id="HFO6462370.1"
                     /translation="MNIPQLTALCLRYHGVLLDASEEVVHVAVVDAPSHELLDALHFA
                     TTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQPKAELLTRTLQSALEQRASDIHI
                     EPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
                     AGNAVSFRIATLPCRGGEKVVLRLLQQVSQALDVNTLGMQPLQLADFAHALQQPQGLV
                     LVTGPTGSGKTVTLYSALQTLNTADINICSVEDPVEIPIAGLNQTQIHPRAGLTFQGV
                     LRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNSTCETLVRLQQMGVA
                     RWMLSSALTLVIAQRLVRKLCPHCRRQQGEPIHIPDNVWPSPLPHWQAPGCVHCYHGF
                     YGRTALFEVLPITPVIRQLISANTDVESLETHARQAGMRTLFENGCLAVEQGLTTFEE
                     LIRVLGMPHGE"
     gene            41097..41216
                     /locus_tag="ACHKTW_001311"
                     /pseudo
     CDS             41097..41216
                     /locus_tag="ACHKTW_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308137.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin biogenesis protein"
     gene            complement(41573..42184)
                     /locus_tag="ACHKTW_001312"
     CDS             complement(41573..42184)
                     /locus_tag="ACHKTW_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO6462371.1"
                     /translation="MDKAEKTVSSAERFIDMEGINRFSERLAMAMGNMTNVELATLCG
                     LSEATVRNYRKGKNFPPLDKLALIAKACNCSLEWLATGKNEDECGKIEKNCDSGSGID
                     QALSSLSPQEKQLILTFIRREGTNNLVRLAAMNTSTISQDAVESIIDALPLRPVLKNA
                     IKIGMAGSEETDKEILRVIEKYNSSNNATQESGAVTVKKNVVG"
     gene            42341..42607
                     /locus_tag="ACHKTW_001313"
     CDS             42341..42607
                     /locus_tag="ACHKTW_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001266279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO6462372.1"
                     /translation="MRVSRQASNNLFVREGCDWSAKAVYAALENKGTDLPELERELGL
                     KSGSMRNVFYRQCRKYEAAIAEKIGVEPAVIWPSRYQVDAREVA"
     gene            42618..>43759
                     /locus_tag="ACHKTW_001314"
     CDS             42618..>43759
                     /locus_tag="ACHKTW_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO6462373.1"
                     /translation="MWVTAKECVGLPDLPMMEHNIRHRLTKAAAGNAAMLRKRKGTKA
                     IEFNIDCLPSGAQQVLRERYALQLMTQKADESPAPVVTKARRSSAVVDAVEAYRGSPQ
                     LMLERLNALTENQRQVADARIAIVSEVLKVAQQPGFSCAKAIRFIVDNLARSQLDERI
                     VAMVETANAKKGNSRALSEITLKRWIAAFNKAQNAAERLLLLAPGKRQEIKAEDINWL
                     PEFLAQYRQSNGRPMTEAYEDFVAEWQHRHADEPYMLDIMPSYDTIRRAMKKLPEVVK
                     QKGRVTGSEYRQLEGFTRRDWSRMPVNYVWIGDGHGMKLKCRHPVHGRPFAPEVTFVI
                     DGGTRFVVGWSLDLAENVFAVAGAIQHGIRHHGKPFLYYSDNGSGET"
BASE COUNT        11025 a        11298 c        10603 g        10833 t
ORIGIN      
        1 tcctaatgaa caatggttgt aaattttggt aacaaatatc attgctcatt aatatctagc
       61 atctgcttat aacatttgtg tcatgaatag tcattcttca aaccagtagt ctgactaatg
      121 gaattttatt caaggatttt tatgtctaaa aaattaggtt ttgccctgtc tggtcttatg
      181 ctggcaatgg ttgcaggtac tgcttctgct gatatggatg gtggtcagtt aaatatcagt
      241 ggtctggttg ttgataacac ctgtgaaacc cgcgttgacg gcggtaataa agatggtctg
      301 atcctgctgc aaactgcaac tgttggtgaa atcgatgctg gcgtgctgaa tgacaccgtt
      361 ggcgctaaag ccaaaccttt cagcatcacc gttgactgca gcaaagcaaa tccgaatcca
      421 ggtagtacag ctaaaatgac cttcggttct gttttctttg gtaatagcaa aggaacgctg
      481 aacaacgaca tgtctattaa caacccgtct gacggcgtga atatcgctct gcacaatatt
      541 gatggttcca ctatcaaaca ggttcaaatt aacaaccctg gcgatgtgta tactaaagcc
      601 ctggatgcaa cgacaaaatc tgctgtttat gattttaaag cgtcttacgt tcgtgccgtc
      661 gcagaccaaa cagcaactgc tggttatgta aaaactaaca ctgcatacac cattacttat
      721 cagtaagtat tcaccaatta aattgattga atgaatatac agggaatagt gttaataatt
      781 tctattttta tattattccc tgttttaatt aactctgtca gggatggttt atgtttttta
      841 acaccaaaca tacaacagct ttatgcttcg taacctgtat ggcttttagt tcatcgtcta
      901 ttgcggacat tgtcatttcg ggtactcgcg taatatataa aagcgatcaa aaaagtgtca
      961 acgtacgtct ggaaaataaa gggaataacc cgttgcttgt ccagagttgg ttagatactg
     1021 gcgatgacaa cgctgagcct ggcagtatta cagtcccttt tactgccacg ccgccagtat
     1081 cgcgtattga tgccaaacgt gggcaaacaa tcaaattaat gtacacagcc agcacctcac
     1141 tgcctaaaga cagagagagc gtgttctggt ttaacgtact ggaagttcca ccaaaaccag
     1201 atgcagaaaa ggtcgcgaat caaagtctgc tgcaactggc atttcgcaca cgtataaaac
     1261 ttttctatcg cccggatgga ttgaagggaa atccctctga agccccgtta gccctgaagt
     1321 ggttctggtc aggttcagaa ggtaaggcgt cattgcgagt gaccaatcca accccttact
     1381 acgtctcttt tagcagtggt gatttagaag ctagcggtaa acgctatccg attgatgtga
     1441 aaatgattgc accatttagt gatgaggtca tgaaagtcac tggccttaat ggcaaagcaa
     1501 gttctgcaaa agtacatttt tacgccatta atgattttgg tggcgccatt gaaggtaatg
     1561 ccagtctgta atcaggcaag gatataattc cgcaggaagc ataacgtgac tataaaacca
     1621 actaaaaatt atcataatca tctgacccgt atcgctacgt tttgcgcgct gctgtattgc
     1681 aatagtgctt tctgtgctga acttgttgaa tatgaccata ccttcctgat ggggcagaat
     1741 gcctcaaata ttgatctcag ccggtatagt gaaggtaacc ctgctatacc gggtatgtac
     1801 gacgtcagtg tttatgtcaa cgatcaacct attattaacc aaagcattac atttattgaa
     1861 attgaaggaa aaaaaaacgc acaagcctgt atcacactta agaatttatt gcagtttcat
     1921 ataaattctc ccgatataaa taacgaaaaa gccgttctgc ttgccaggga tgaaacactc
     1981 ggcaattgcc ttaatttgac ggaaattatc cctcaggctt ctgttcgtta tgacgttaac
     2041 gagcaacgtc tggatataga cgttcctcag gcatgggtaa tgaaaaatta ccaaaactat
     2101 gttgatccat cgttatggga aaatggcatt aatgcggcca tgttgtcata caacctcaac
     2161 ggatatcata gtgaaactcc tggtcgaaga aatgacagta tttatgctgc ttttaacggt
     2221 gggatgaatt taggtgcatg gcgactgcgt gcctcaggca actacaactg gatgaccgat
     2281 tctggcagta attatgattt taagaatcgg tatatacagc gtgatatcgc ctcgctgcgt
     2341 tctcaactca ttcttggtga gtcttataca acgggcgaaa cctttgattc tgtcagtatc
     2401 cgcggcattc gtttatacag cgacagccgc atgctgcctc cgacattagc gagttttgcg
     2461 cctatcattc atggcgttgc taataccaac gccaaagtca ctattacgca aggtggctat
     2521 aagatttatg aaacgactgt gccgccaggc gctttcgtca ttgatgatct gagtccgtca
     2581 ggttacggca gcgatcttat tgttactgtc gaagaatccg atggctcaaa gcggacattc
     2641 tcgcaacctt tttcatcggt cgttcaaatg ttacgccctg gcgttggacg ttgggatatt
     2701 agcggcggcc aggtcttaaa agatgatatt caggatgagc ctaatttatt tcaggccagc
     2761 tactactacg gtctgaataa ctatctaacg ggttatactg gtattcagat taccgataat
     2821 aactataccg ctggtttgtt aggtcttggt ctgaatactt cagtcggtgc attttctttc
     2881 gatgtgactc actccaatgt tcgtataccg gatgataaaa catatcaggg acaaagttat
     2941 cgtgtttcct ggaacaagtt attcgaagaa acaagtactt cactgaatat cgcggcctat
     3001 cgctattcga cacagaatta ccttggtctt aatgatgcac taactctaat tgatgaagtg
     3061 aaacatcccg aacaagatct tgaaccgaaa tccatgcgta attactcgcg catgaaaaat
     3121 caggttacgg tcagtattaa ccaaccgcta aaatttgaga aaaaagatta cggttcattt
     3181 tatctttccg gaagttggtc cgattactgg gcttccggac aaaatcgtag caattactct
     3241 attggctaca gtaacagtgc atcctggggc agctacagtg tcagtgccca gcgttcatgg
     3301 aatgaagacg gcgacactga cgatagcgtt tatctcagtt tcaccattcc tattgaaaaa
     3361 ttacttggca ctgaacaacg gacttcaggt ttccagagta ttgatactca aatgagcagt
     3421 gactttaagg gtaataacca actcaacgtc agtagcagtg gctatagtga taacgctcgc
     3481 gtcagttata gcgtaaatac tggctatacg atgaataaag ccagcaaaga tttgagttat
     3541 gttgggggtt atgccagcta tgaatcaccg tggggaagcc tggcaggttc agtttctgca
     3601 aatagcgata acagtcgtca agtgtctctc agcaccgacg gtggttttgt attgcatagc
     3661 ggcggcctga ccttcagtaa tgacagtttt agcgactccg atacactggc ggtagttcag
     3721 gctccaggtg ctcaaggagc gcgaataaat tatggcaaca gtactatcga tcgatggggt
     3781 tatggtgtca ccagcgctct ttctccttat catgaaaacc gtatcgcgct ggatatcaac
     3841 gatcttgaga acgatgttga attaaaaagt accagtgcag tagctgtacc gcgtcagggt
     3901 tcagtcgtct ttgctgattt tgaaaccgtg caagggcaat cagccattat gaacatcaca
     3961 cgaagtgatg gtaaaaatat tccatttgct gcagatattt atgatgagaa aggcaatgtc
     4021 attggtaatg ttggacaggg tggacaagca tttgttcgtg gtattgagca gcagggaaat
     4081 atcagcatta aatggctcga agaaagtaaa cccgtaagtt gtcttgcgca ttatcaacaa
     4141 agttcagaag cagaaaaaat agcgcaatct attattctga atggaattcg gtgtcagatt
     4201 cagtaactac aagggacgtc aaatgataaa aataacgcca cataaaatag cgatactgat
     4261 gggaatatta ttatcaccat cagtatttgc aacggatatt aatgtagagt ttacagccac
     4321 tgtcaaagcg acaacctgta acatcacact tactggtaat aacgtcacga atgatggcaa
     4381 taataactac acattgagaa tccctaagat gggtctggat aagatcgcga ataaaacgac
     4441 agaatctcag gctgatttta aactggttgc tagtgggtgc agcagtggca tcagttggat
     4501 tgataccact ctgaccggaa atgcatcatc aagctcacct aagcttatta taccgcagtc
     4561 tggtgattca tcttcgacga caagtaatat cggtatgggt ttcaaaaaac ggactactga
     4621 tgatgccact ttccttaaac ctaacagtgc ggaaaagata cgctggagca cagacgagat
     4681 gcagcccgat aagggtcttg aaatgaccgt tgcgctacgt gaaacagatg cagggcaagg
     4741 cgtaccgggg aattttcgtg cactggcaac gtttaatttc atctatcaat aagaaggata
     4801 attaagatga catttaaaaa tttacgttat ggattatcca gcagtgtcgt tttggctgcc
     4861 tcattgttca gcgtaccctc ttatgcggca acagacagca ttgggctgac cgtgattact
     4921 accgtagaaa taggtacttg tactgctaca ttagtaaatg actctgatca ggacatttct
     4981 gttgttgact ttggcgatgt atatatttct gaaatcaatg ccaagaccaa agttaaaaca
     5041 ttcaaactca aattcaaaga ctgtgcgggt atccccaata aaaaagcaca aataaaatta
     5101 accaagcgag ccacatgcga aggtactgct aatgacggtg cggggttcgc aaatggatcc
     5161 acagccgcag ataaagcaag tgccgtcgcc gttgaagtct ggagcaccga aactccggca
     5221 acagggagcg caacacaatt tagttgtgtg acaccagcat ctcaagaggt cactatctcc
     5281 aatgcagcca acgcggttgt ttattatccg atgagtgctc gactggtcgt cgaaaaaaat
     5341 aaaaccgtaa gcaatgtcac tgcgggtaaa ttttctgcac ctgccacatt tacagtaacc
     5401 tataactaac agactgacag atccaggaac tatatatgca tcccactcaa cgtaagctga
     5461 tgaagagaat aattctgttt ctgtcactac agtttcccat tacctatcca gccatcgctg
     5521 ggcaggatat tgaccttgtt gccaatgtaa aaaacagcac ttgcaaaagc ggaatcagta
     5581 accagggtaa tattgatctt ggcgtcgttg gggtgggata tttttcagat aatgttactc
     5641 ctgaaagcta tcaaccaggt ggaaaagagt tcactgtcac agtatcagac tgtgcattac
     5701 agggaactgg cgatgtgctt aatcagttac atattgattt tagagccctt agcggtgtca
     5761 tggctgcagg ttcaaggcaa atatttgcca atgaagtcgc aaccggggct aaaaatgtcg
     5821 gtgtcgttct cttttccatt caggacccga ccaatatatt caatgttatt agttcagcag
     5881 gaaattctcg ttccgtgtat ccagtaatga cttctgccct gcataattct tcatggaaat
     5941 tttatgccag aatgcaaaaa atcgatcccg cgctggatgt catatctggc caggtaatga
     6001 gtcatatatt agttgatgtg tactacgaat aatagatcaa aacaaataac tgcgactgtt
     6061 acagcagtag atggtggagt gataatgaag aaattcttca gacattttct ttttttaatt
     6121 ctgtgcttga gttgttatac cgcgagtgct ggcactgatg ataacgtagg atatattgtt
     6181 gggaatagtt acggcgttgg tccgtcagat caaaaatgga gagaaacggg gccgaatggc
     6241 gatgctactg tcatattcag atatgcaaca agtacaaata atttagtttt ctacaaaccg
     6301 acgcagcttg gacctacagg tgtaaaatta cagtggagtc agttagatac cgcttctggt
     6361 ggtggttttc tttattgcaa cagaagtgat agcacaagtg gtagcgcaat gcgtattgaa
     6421 aatgcaatgg ttgactcagg taaaatgtat ggctcccata aattatttaa tacatcagtt
     6481 cctggtttgt attacacatt attaatttca aacatgtggt cagcttacgg taccgtaact
     6541 aacgttagtt cacctgggat atacattggt gactctgcag aacaatattt ttcgtggtat
     6601 aatccaagcg aagacgtgtt atattggagt tgcaataatg cgaatagcac ccgtaaatac
     6661 tgggctgtag gtggtattta tcagaccctt acaattgaat tctatacaga tacaaacttt
     6721 gatccaacag taactcaaca gatcaaatta tccagctcat caaattatct gtattcattt
     6781 aaagcctatg gcgcaggcca aggtataaat gagcatagtt attttatcaa aatcgatttc
     6841 gatctgctca atgtcaagtt gactaatccc acttgtttta ccgctatgct tagtggaact
     6901 tctgtgactg gttctacggt aaaaatgggt gaatatagtg cagaacaaat cagaaacggt
     6961 gccacaccgg ttccttttga tatttcactt caaaactgcg ttcgtgtgac taatattgag
     7021 acaaaattag tttcaacaaa ggttggtact gaaaacgggc aactccttgg taatactctc
     7081 acaggtaatg acgcagcaaa aggagtcggt gtactcatag aaggtttagc aactagtaaa
     7141 aatcctctaa tgacattgaa acctaatgat tcaaattctg tttataaaga ttacgacccg
     7201 agaggcaaag acgatacaac aggaggagtt tacccggatc aagatacagg tataacatac
     7261 cctctccatt tccaggccac gctacaacag gatggaacta taccaataga agctggtgag
     7321 tttaaagcca ccagtacttt ccaggtaacc tacccttaat aagtccaccg caccgccatc
     7381 atctggcggt gcggtaattg ataaatcatt gcccccacca atgtgactga taaaatgcca
     7441 ggcaacacga catcatttat caggacacgt tatgaaaccg accaccattg cttcacttca
     7501 gaaatgtaag caggataaaa agcgtttcgc gaccatcacc gcttacgact acagcttcgc
     7561 gaaactattt gctgatgaag gacttaacgt catgctggta ggcgattcgc tgggcatgac
     7621 ggttcagggc cacgactcca cccttcccgt taccgtcgag gatatcgcct atcataccgc
     7681 cgccgtacgt cgcggcgcgc caaactgcct gctgttggct gacctgccgt ttatggcgta
     7741 tgccacgccg gaacaagcct ttgaaaacgc cgcaacggtt atgcgtgccg gtgccaatat
     7801 ggtcaaaatt gagggcggtg agtggctggt cgaaaccgta aaaatgctga ccgaacgtgc
     7861 cgttcctgta tgtggtcact taggtttaac cccacagtca gtgaatattt tcggtggcta
     7921 caaagttcag gggcgcggcg atgaagcggg cgatcaactg ctcagcgatg cattagcctt
     7981 agaagccgct ggggcacagc tgctggtgct ggaatgcgtg ccggttgaac tggcaaaacg
     8041 tattaccgaa gcactggcga tcccggttat tggcattggc gcaggcaacg tcactgacgg
     8101 gcagatcctc gtgatgcacg acgccttcgg cattaccggc ggtcacattc ctaaattcgc
     8161 taaaaatttc ctcgccgaaa cgggcgacat ccgcgcggct gtgcggcagt atatggctga
     8221 agtggagtcc ggcgtttatc cgggcgaaga acacagtttc cattaaggag tcacgttgtg
     8281 ttaattatcg aaaccctgcc gctgctgcgt cagcaaattc gccgcctgcg tatggaaggc
     8341 aagcgcgtgg cgctggtgcc tactatgggt aatctgcacg atggccatat gaagctggtc
     8401 gacgaagcca aagcccgcgc cgatgtggtc gtcgtcagta ttttcgttaa cccgatgcag
     8461 ttcgaccgcc cggaagatct ggcacgttat ccacggacct tgcaggagga ctgcgagaag
     8521 ctaaacaaac gtaaagtgga tttagttttc gccccttcgg taaaagagat ctacccgaac
     8581 ggtactgaaa cccatactta cgttgacgtt cccgggcttt cgaccatgct ggaaggcgcg
     8641 agccgtccgg ggcattttcg cggcgtttcg actatcgtca gcaagctgtt taacctggtc
     8701 cagccggaca tcgcctgctt cggtgaaaag gactttcagc aactggcgct gatccgcaaa
     8761 atggttgccg atatgggctt cgatattgag attgtcggtg tgccaattat gcgcgccaaa
     8821 gatggccttg cgctcagttc ccgtaatggt tatctgacgg cggaacaacg caaaattgcg
     8881 cccggtctgt acaaagtttt aagttcaatt gccgacaaat tgcaggctgg ggaacgggat
     8941 ctcgatgaaa ttattaccat tgcggggcaa gaactgaatg aaaaaggctt ccgcgccgat
     9001 gatattcaga ttcgcgatgc cgacacattg ctggaagttt ctgaaaccag caaacgggca
     9061 gtaattctgg tagccgcctg gcttggcgat gctcgcctga tcgacaacaa aatggtcgag
     9121 ctggcgtaat acttaactgg cgctacggct gatggcgcca gttattaatt taccccacgt
     9181 catctgtcat aacacaggta acttaaataa ggttaattac cttatcaatc tcagcaagag
     9241 gtaaatttgc catctctgca acgtcttccc gagacattcc tttacttaga agacgcaggg
     9301 cgaattcttg cctttcctcc tgtcttccct gctgaatccc cttctcaatc cctttctctt
     9361 caaaccactg cgccagcgtc atcatagact cccctcccgt ttccctgtct ctcagcacac
     9421 cataaaacaa atccgcttgt tcagtatgac cgcgttgcag catatagttt tgcatggcaa
     9481 ctaactgact tccgctagtg tacccttcgt caatcagcgt gaccagttgc tccagcaata
     9541 acattaagtc gcgctggcga atatgttttt gcagtagttc gagaatcgca atccgccgat
     9601 gttgcatgat ttcgtcatcc ggtgtgatgg tgatatccac cagcgggaaa ggactgttat
     9661 agacgcgtcg tgccagctcc ggcgagtaaa atatatcaaa ccagcacatt gagagcggat
     9721 aaggtgtggc ctcgccctga taaaacagta tcggcaccac cagcggcagc ttatcgtgat
     9781 ccgcctccag atgccggtgc atggcagcaa tagaataacg catcatgcga aaggccattt
     9841 tcttatccgg cttgctttgg tgttcaatca caacatgcag ataaccggga ttgccctgca
     9901 tttgcacgga atagagcacg tccgtgctgt gtcctttcag gctctcttca atgaaactcc
     9961 ccgactctaa atgaagcgtg ttgaggtcac aaagttctcg taattccact ggcaaatgta
    10021 tctccagaaa gtcgcgagcc gtctccgcat gcattaaaaa ttgttaaata ccgcgtcatg
    10081 cggtgtggta ctcggtgcat ccatcttccc tgtcttccgt gttgtcttgc gtgaaggcag
    10141 tatccagtca aggcgcgccg tcgtcagccg cctctttagt gtttaccgag cagcgttcag
    10201 agaaatttca attcgttaca ggcgatacat ggcacgcttc ggcgcggtac gcaaactgct
    10261 gacgcaaatg ccatcagatg agtagacact catcaaaaat cgggcaatac tgcgtgagaa
    10321 tttcctcaaa gcaggcaccg cctgcttcgt taacgacagg gtagaaaggt agaagttatg
    10381 attcgcacga tgctgcaggg caaactccac cgcgtgaaag tgactcatgc ggacctgcac
    10441 tatgaaggtt cttgcgccat tgaccaggat tttcttgacg cagccggtat tctcgaaaac
    10501 gaagccatcg atatctggaa tgtcaccaac ggcaagcgtt tctccactta tgccatcgcg
    10561 gcagaacgcg gttcgagaat tatttctgtt aacggtgcgg cggcccactg cgccagcgtc
    10621 ggcgatattg tcatcatcgc cagcttcgtt accatgccag atgaagaagc tcgcacctgg
    10681 cgacccaatg tcgcctattt tgaaggcgac aatgaaatga aacgtaccgc gaaagcgatt
    10741 ccggtacagg ttgcttgatt gttatccctg cggctggtta ctcaccagcc gcgacatcgt
    10801 ctccagcgaa tccgttctta agatataaag tcgttttagt aacaacggat tatcccccgg
    10861 ttttactttg cctttcatgg ttgtcaccgc caggtgaaat ccggcatcgt ttgctgcctt
    10921 cacggctttg tcattaaatc cgccaaacgg atacgaaaga taccagacat gcggattaaa
    10981 ttgcgccaga gcgcggcgtg aacgtgcaaa atcaaacaga atattgtgct cactacggct
    11041 cagtaatatg ggtcggcgat agccatccac ccgatgcaaa aaatgggtat gtgactgaaa
    11101 atcaaaaaca tcgcgaattt cgttaagctc agaaacgctc ataaattgca gcgattttgg
    11161 gttccacttc tgcgggtgac gtttgatgcg cgaggtaaca acaaacgccg tcgccttcat
    11221 gccatattgt ttcaatacag gatacgcata gcggctcacc gacttgaggc catcatcaaa
    11281 ggtaatcacc accgctcgcg cagggagatt gatcttattc ttcacgtaac cttccagctg
    11341 cgccatgctc agtgtcgcgt atcccctgtc gcgcagccag gccatctggt tattgaaagc
    11401 gcgtaccgag gtggtcgtcg aagtatggcg aaaacgggtg ttttcttcat cgcgcagaat
    11461 atggtgatag gttagcaccg gcaggccatt atcgggttgg gcatccagcg cgctgatata
    11521 ggcgagtcga tcgccaatac ggatctgata ccaggtttga tttaacctgt ctttcagttt
    11581 atgcaaaatc gggtagcgca aattgtccgc cagtacccca aatggcgcac tgcccgcact
    11641 cggcgcgtta tagaccggcg tatctttcca ggtaattaag ttctgattac tcagcggctt
    11701 gttgaggtca cccaaaccgt cttcaacttt ttgtcgcccc tgaactggct cgagatgccc
    11761 tttatcgata aaccctttac caaagccaaa attaaatgcg taataacttg cggcagtggg
    11821 ctccacggca atgatttgcc cggcccgaat attccccacg gtcaccatct tgtcgccaat
    11881 ttgcgcccag atcgcagcat tttcgatggt ttgcatataa cgggcaggta acgcggcact
    11941 gacacttgcg gtaaacagca tcagcaggag aataacagct tgtttgtaca tgaaaaaata
    12001 accgagggtg aaacaacgct tttattgtag caaaagcgct ataagaatcg tgggttaata
    12061 ctcatacaaa ttatgtttga ggacgaaagg cggatttttt tgttgttggt gccaaagact
    12121 actcacctcc ggcccaccca gttcgacaat acgctcgcga aactctgaac tagtcatggt
    12181 ttcacggcaa gccatcatct gttcaattaa cggtaacgtg acaccagaaa tcacttcgca
    12241 acgggagtgt ttgtggctta ataatgaagc cacgcggtac ggtggcgctc cggctatatc
    12301 ggttaagaag atgacaccct caccagagtc cgcttcatgc agagcatcgc acatcatgcg
    12361 gctgagcata ttagagctta acccacgcca gaaattcacg gcccggcact gaagaagcgc
    12421 cccatgtttt ttctccagcg catccagtat ctcttgcgcc cggtcatcgt gacaggtaat
    12481 tacccaacct aacatcgcct tcctccttag ccgaaaatca gtctaccctg accattgttg
    12541 ataaagggtg atagccgtca aaactcctct cccccaaatc cagaggagag gaaatagcct
    12601 tagctacgca aaccacgtcc acgttggatc agcgaccaac agatcaaata aaacgccaca
    12661 ataaagacca ccagtacgcc aaaggtagtg accagcggaa catcattgat accgaggaag
    12721 ccgtagcgga aaccactgat catataaacg attgggttca ggtgcgacag cccttgccag
    12781 aacggcggca acaaagtcag tgagtaaaag accccaccca aatacgtgag tggcgttaac
    12841 acaaaggttg gcaccaggct gatgtcatcg aacgttttgg caaacacacc gttcagcaaa
    12901 cccgcaaggg agaacaacac cgccgtgagc accagggtta aggcaacgaa tacccacgaa
    12961 tgcacctgaa acggcacaaa aaacagtgaa attgccgtca ccagaatgcc aacaaacaga
    13021 ccacgcgcca cgccaccgcc gacatatccg gcaataatga cgtgagtcgg aaccggcgct
    13081 accagcagct cttcaatatt acgctggaac ttggcaccaa aaaatgatga cgcaacgttg
    13141 gcgtaggcat tggtgatcac cgacatcatg atcagccccg gtacgatgaa ctgcatatag
    13201 ctgaagccat gcatatcgcc aatacgcgaa ccaatcaggt taccgaagat aataaagtaa
    13261 agggtcatgg tgatgactgg cggcaccagc gtctgcaccc agatacgcat aaagcgatgg
    13321 atctctttcg cccagatgct ttttagcgcc acccagtaaa gatgcatcat gcgcgatctc
    13381 cttgtttttc attaaccagt gaaacaaaca gctcttccag acggttagct ttgttacgca
    13441 tacttaatac ctgaatgccc tgctcactta actgcgtaaa tacgctgttg atcccctgct
    13501 cacgcagcac ttcaacttcc agcgtcgcgg tatcgaccag tcgatactga tagccatcga
    13561 gcttcggtaa cgggcttttc ggtgcgagat cgagaataaa ggtttccgat ttcagcttcg
    13621 ccagcagcgc cttcatcgag gtattttcca ccagctcacc gtgttgaata atgccgatat
    13681 tgcggcacag catttctgct tcttccaggt agtgtgtggt gagaatgatg gtggtgcctt
    13741 tgtcgtttaa atccttcaaa aagccccaca ttgagcggcg aagttcaata tccacgcctg
    13801 cggtcggttc gtcgagaatc agtagtttag gttcatgcat taacgcacgg gcaatcatta
    13861 aacggcgctt catcccgcca gataacatac gcgcacgttc gttgcgtttt ccccatagat
    13921 cgagttgttt aagatacttt tcgctgcgga tgtacgcttc tttgcgctcc acgccatagt
    13981 accctgcctg attcaccaca atttgctgca cggtttcaaa cgggttgaag ttaaattcct
    14041 gcggcaccag tcccaactga cgtttagcgt tcacgacatc cttctcgaga tcgtaaccaa
    14101 atacgctgac ccgcccggag gttttattta ccagagagct gataataccg atagtggtcg
    14161 atttcccggc cccgttcggc ccgagaagcg cataaaaatc acccgcttcg acctgcaaat
    14221 ctatcccacg aagcgcctga acgccgcctg gataggtttt tttaagctgt tgaagttcca
    14281 gtgcaatggt cataaatttt tacttacctt acgttcttac actttatctg tggtttaaat
    14341 cgtcccggag ttgccctata ttagccaaac gtaattattt ggttacaggt cgttaacctc
    14401 catgaaagac atagatacac tcatcagcaa caatgcacta tggtcaaaaa tgctggtgga
    14461 agaggatccc gggttttttg agaaactggc acaagcgcaa aaaccgcgct ttctatggat
    14521 tggatgttcc gacagtcgcg ttcctgcaga acgtttaacc ggtcttgagc cgggcgaact
    14581 ctttgttcac cgtaatgttg ctaacctggt cattcacact gacctgaact gcctttccgt
    14641 ggttcagtat gcagtggatg tactcgaagt tgaacacatt attatctgtg gccactacgg
    14701 ttgcggcggc gtacaagccg cagttgaaaa cccggaactg gggcttatca acaactggct
    14761 gctgcatatc cgcgatatct ggttcaaaca tagctcattg ctcggcgaaa tgccgcaaga
    14821 gcgccgtctg gataccttgt gtgaactgaa cgtcatggaa caggtgtata acctgggcca
    14881 ctccaccatt atgcaatcag cgtggaaacg cgggcagaaa gttaccattc acggctgggc
    14941 ctacggcatt cacgacggct tgctgcgtga tctggatgtc acagcgacca accgcgaaac
    15001 ccttgagcaa cgttaccgtc acgggatttc caacctcaag ctgaaacacg ccaaccacaa
    15061 ataaaaatgc catgccggat gcaacacatc cggcaacttc acacttactc gtccagcaga
    15121 atcactttgc cgatatacgg cagatgacgg taacgctgtg cgtaatcaat gccgtaaccc
    15181 accacaaact catccgggat cgagaaaccg ataaattcta ccgggacgtt cacttcacga
    15241 cgggacggtt tatccagcag cgtacaaatc gccagcgact tcggttcgcg caggcttaag
    15301 atctcacgca ctttcgacag tgtattcccc gagtcgatga tatcttcaac aatcagcacg
    15361 tccttgccac ggatatcttc atccagatct ttgaggattt tcacatcacg ggtggtggac
    15421 atgccgctac cgtagctgga ggcggtcata aagtcgactt catgagatac ctgaacttca
    15481 cggcacaggt ccgccataaa cataaatgag ccacgcagca gaccaaccag caccatatcg
    15541 ctgccgctgt ctttgtaacg ctcagtaatc tgacgaccca gttcggcgat acgcgcttta
    15601 atctccgctt cggggatcat tacttctaca gtatgtttca tatctctaac catatgattt
    15661 aaaaacaaat cacttcgcac gggcaggttt tggcaccgca acaaagcgca agacacgcag
    15721 tataccagta aaactattca ttcgcggaat ctgttaataa agctcgaatt gtgatgacga
    15781 tcacacatgt taaaacctat aattaattgc tactaaaata ttaatgaatt gaaatggtgt
    15841 ctctttatgg caattaacaa tacaggctcg cgacgattac tcgtcacgct aacagccctt
    15901 tttgcagcgc tttgcgggct gtatctactc attggcggag gctggctggt cgcgattggc
    15961 ggctcctggt actaccctat cgctggtctt gtgatgctcg gcgtcgcctg gatgctgtgg
    16021 cgcagtaaac gtgccgcgct ttggctgtac gcagccctgc tgctcggcac catgatttgg
    16081 ggcgtctggg aagttggttt cgacttctgg gcgctgactc cgcgcagcga cattctggtc
    16141 ttcttcggca tctggctgat cctgccgttt gtctggcgtc gcctggtcat tcctgccagc
    16201 ggcgcagttg ccgcactggt ggtggcactg ctgattagcg gtggtatcct gacctgggcc
    16261 ggatttaacg atccgcagga aatcaacggc accttaagcg ccaatgccac acctgcggaa
    16321 gctatctcac ccgttgccga tcaggactgg cctgcctatg gtcgcaatca ggaaggtcaa
    16381 cgcttttcac ctctgaaaca aattaacgcc gataacgtcc ataatctgaa agaagcctgg
    16441 gtgttccgta ctggcgatgt gaagcagccg aacgatccgg gcgaaatcac caatgaagtg
    16501 acgccgatta aagtgggcga caccctttac ctgtgtaccg ctcaccagcg cctgtttgcg
    16561 ctcgatgccg ccagcggcaa agagaaatgg cattacgatc ctgagctgaa aactaacgag
    16621 tctttccagc acgtaacctg ccgtggcgtc tcttatcatg aagccaaagc agaaacggct
    16681 tcgccggaag tgatggcgga ttgcccgcgt cgtatcattc ttccggtcaa tgatggtcga
    16741 ctgattgcga ttaacgctga aaacggcaaa ctgtgcgaaa ccttcgccaa taaaggcgtg
    16801 ctcaatctgc aaagcaatat gccagacacc aaaccgggtc tgtatgaacc gacttcgcca
    16861 ccgattatca ccgataaaac catcgtgatg gccggttcag ttaccgataa cttctcaacc
    16921 cgcgaaacgt ctggcgtgat ccgtggtttt gatgtcaaca ccggggagct gctgtgggct
    16981 tttgatcccg gcgcgaaaga tccgaacgca atcccgtctg acgaacacac ctttaccttt
    17041 aactcgccaa actcctgggc accagcggcc tatgacgcga agctggatct ggtctatctg
    17101 ccgatgggcg tgaccacgcc ggatatctgg ggcggtaacc gcacaccgga acaggaacgt
    17161 tatgccagct cgattctggc gctgaatgcc actaccggga aactggcgtg gagctaccag
    17221 accgttcacc acgacctgtg ggacatggat cttccggcac agccgacgct ggcggacatc
    17281 accgttaatg gtcagaaagt gccagttatt tacgctccgg cgaaaaccgg caacattttt
    17341 gtgctcgatc gtcgcaatgg cgaactggtg gttccggccc cggaaaaacc ggtgccgcaa
    17401 ggtgctgcga aaggcgatta cgttactccg acccagccgt tctctgaact gagcttccgt
    17461 ccgaagaaag atttgagcgg tgcggatatg tggggagcca ccatgtttga ccaactggtg
    17521 tgccgcgtga tgttccacca gttgcgctat gaaggcattt tcacgccgcc atctgaacag
    17581 ggcacgctgg tcttcccggg taacctggga atgtttgaat ggggcgggat ttccgttgat
    17641 ccaaatcgtg aagtggcaat tgccaaccca atggcactgc cgtttgtttc gaaactgatc
    17701 ccacgcggtc cgggtaaccc gatggagcag ccgaaagatg ccaaaggcac gggtacggaa
    17761 tccggcattc agccacagta cggtgtaccg tatggtgtca cgctcaaccc gttcctctca
    17821 ccgtttggtc tgccgtgtaa acagccagca tggggttata tctcggcgct ggacctgaaa
    17881 accaatgaag tggtgtggaa gaaacgtatt ggtacgccgc aggacagtat gccgttcccg
    17941 atgcctgtac cggtgccgtt caatatgggt atgccgatgc tgggcgggcc aatctccacg
    18001 gcgggtaacg tgctgtttat cgccgctacg gcagataact acctgcgcgc ttacaacatg
    18061 agcaacggtg aaaaactgtg gcagggtcgt ttaccagcgg gtggtcaggc tacgccaatg
    18121 acctatgaag tgaatggtaa gcagtatgtg gtgatctccg caggcggtca cggttcattt
    18181 ggtacgaaga tgggcgacta tattgtggct tatgcgctgc cggatgatgt gaagtaacca
    18241 taatgcccgg agaaatccgg gcatatttcc gaatacggtc tttttatacc gtaaacccta
    18301 acatcatccc cgtatcttca tgctccagca gatggcagtg cgccatataa gcatgttctt
    18361 tcggtgcgtc gtgattaaac ttcaccagca cttcactgac attgccttct accttaacgg
    18421 tatctttcca gcccgcgcga tgagccgctg gcggtttgcc attttctgac aagatacgga
    18481 actgggtgcc gtgaatatgg aacggatgca gcatcatgtc gcccacgcca gagataaccc
    18541 aacgttcgta ctgccctttc gccgccgcaa acatcggctt attcatatca aacgcctgac
    18601 cgttgatttt attggcatgg tggaaatcga acttcccgcc gtggttcata tgattcatat
    18661 taccgtgccc catatggccc atcatctggc tgtgatccat tccagccatc gcctgatcgc
    18721 catatttctc cattagcatc tgcataccca tcatatcgag catcgggtcc atagaaagtt
    18781 gcagcttgcg taccgtcagc ccttccagcg aaggtaacgc aggcaggcta cttaatgtgt
    18841 ctggcaaagc accggaggca ctaatagcaa tcggctgaat ccgcattacc ggatgcggct
    18901 tgtcaaacgg cgcaatcgcc atccccatct ggctgaccgg tagcgtcacc aggtcaaagg
    18961 gtttgttgtc gttaacctcc accagcactt caaaacgctc gcccatcagc accggcagct
    19021 cgctcacctt caccggttca ggtagcagac caccgtcgct ggcaatcaca tacagcgggc
    19081 gattgtcgct ggtggcgaaa ttgagcgagc gggcattaca gccattgagc aaacgcaggc
    19141 gcagccaacc acgcggggca gcgtgttgtg ggtagattgc accgttggtc agcagcgtat
    19201 cgccaaacca gcccacggcg gcggtcatca catccagttg ataatcaatc tgcccatcag
    19261 cgttaaattt tttatcctga acaatcaccg gaacatcatc gataccccac tgttttggca
    19321 gcattaattt caggatctcg tcatcttcaa ttaccaccag cccagccaac cccatcgcca
    19381 cctgtcgccc ggtcttgcca tgttgatgtg gatggaacca gcaagtagcg gcaggttgat
    19441 caacgttcaa cgtcaccgag cgcttgccac ctggcagaat aattccctgc gggccgccgt
    19501 cgacttcacc cggtacttcc agcccgtgcc agtgcaacgt cgtctcttcc gtcagttggt
    19561 tatagatatc aaccgttacc gctttgccgc gctgtaattt caccgccggc cccagcagat
    19621 tgccgttata gccccaggta gttgcagttt tctcgccaaa ggtagactga cctgcgccaa
    19681 tagttaactg aatgcgatta cgggcatcgg tcgtgagcaa atcagggatc ggtaacgttg
    19741 ggcgttccgc cgcaaagact gcgcggctcc acagcggcaa ggctgaagcc acacccagcg
    19801 cgacggaata ttttaagaaa tcacgacgtt gcatagttat ttccttattc ttaagcaggc
    19861 gaaacaaaat caaacgttga gcatagtcct tccccttacg ggaaggtcaa gccgcagaca
    19921 caatttaatc gacaataatg gtcgtcgcct cgctcgcagt gtgctaacgt ttatcttctt
    19981 taagcccctg gtagaagcaa tgaagacgtt tttcagaaca gtgttattcg gcagcctgat
    20041 ggccgtttgc gcaaacagtt acgcgctcag cgagtctgaa gccgaagata tggccgattt
    20101 aacggcagtt tttgtctttc tgaagaacga ttgtggttac cagaacttac ctaacgggca
    20161 aattcgtcgc gcactggtct ttttcgccca gcaaaaccag tgggatctca gtaattacga
    20221 caccttcgac atgaaagccc tcggtgaaga cagctaccgc gatctcagcg gcattggcat
    20281 tcccgtcgct aaaaagtgca aagccctggc ccgcgattcc ttaagcctgc ttgcctacgt
    20341 caaataatcc ctgatacctt tttgtagaaa tattgaccgt gcatccgcgg tcaatgttag
    20401 ctattatgtt gcgccctttt tttacgggtg ttaacaaagg aggtatcaac ccatggccga
    20461 aaaaaaacag tggcatgaaa cgctacacga ccagtttggg cagtactttg cggtagataa
    20521 cgttctgtat catgaaaaga ccgatcacca agatctgatc atttttgaga acgctgcatt
    20581 tggtcgcgta atggcgctgg atggcgtagt acaaaccacc gagcgcgacg agtttatcta
    20641 tcatgagatg atgacccatg ttccgctact ggcccacggt cacgcgaaac atgtgctgat
    20701 tatcggcggc ggcgacggtg ccatgctgcg tgaagtaacc cgacataaaa acgttgagtc
    20761 aatcacgatg gtggaaatcg atgcgggtgt cgtgtcgttc tgccgtcagt atctgcccaa
    20821 ccataacgcc ggtagctacg acgatccgcg ctttaagctg gtgatcgacg atggcgtcaa
    20881 tttcgttaat caaaccagcc agacctttga tgtcattatc tccgactgca ccgatcctat
    20941 cggtcccggc gaaagtctgt tcacctcagc attttatgaa ggctgcaaac gttgcctgaa
    21001 tcctggcggt atcttcgtcg cacaaaacgg cgtctgcttt ttacagcagg aagaagccat
    21061 cgacagccat cgcaaactca gccattactt cagcgacgtt ggcttttatc aggcggcgat
    21121 cccgacctat tacggcggta tcatgacttt tgcatgggcg acagataacg acgccttacg
    21181 ccatctctca accgaaatta ttcaggcgcg ttttctcgct tctggcctga aatgccgtta
    21241 ttacaatccg gcaatccata cggcagcttt tgccttacct cagtatctgc aagacgcact
    21301 ggcttcacag ccgtcctaag gagaagataa gaaattgaaa aaactgaaac tgcatggctt
    21361 taataatctg accaaaagtc tgagtttttg tatttacgat atctgctacg ccaaaactgc
    21421 cgaagagcgc gacggttata ttgcttatat cgatgaactc tataatgcca accgtctgac
    21481 cgaaatcctg tcagaaacct gttccattat cggggccaat attcttaaca tcgcccgcca
    21541 ggattacgaa ccacagggtg ccagcgtcac tattctggtg agtgaagaac cggttgaccc
    21601 gaaactcatc gacaaaacag aacaccccgg cccactgcca gaaacggtgg ttgcccatct
    21661 cgataaaagt catatttgcg tacataccta cccggaaagt catcctgaag gcggtttatg
    21721 taccttccgc gccgatattg aagtctctac ctgcggcgtg atttctccgc tgaaggcgct
    21781 gaattacctg atccaccagc ttgagtccga tatcgtaacc attgattatc gcgtgcgcgg
    21841 ttttactcgc gacattaacg gtatgaagca ctttatcgac catgagatta attcgattca
    21901 gaactttatg tctgacgata tgaaggcgct gtatgacatg gtggatgtga acgtctatca
    21961 ggaaaatatc ttccatacca agatgttgct taaagagttc gaccttaagc actacatgtt
    22021 ccacaccaaa ccggaagact taaccgacag cgagcgccag gaaattaccg ctgcgctgtg
    22081 gaaagagatg cgcgagattt attacgggcg caatatgcca gctgtttaac ggctctggcg
    22141 gagctcccag gctccgccag atttatttac ttctgctgca cgaaattacg gtaagccgcc
    22201 acgacctgca gaaaatcctc aacgccgcat agcgacaggc tttcttcgtc gtagtagttc
    22261 atcccctctt ccatttcatc gccagcgaat tccagttgat tggcgcgaac catcacctct
    22321 tcaccgtcca tccacagagt gtactcatgc cctgcccgtt gccaggaccg ttcgctacct
    22381 ttcagtgcgt gcgctgcttg ttccacttca tcaagcaagg ccaggttttc tttcacctct
    22441 tcattaaacc agtgcccgac cacttcatgc cccatggaca tacgcacctt taccactccg
    22501 gtaatatcgc gcagaaattc gtaatccata gtgtgttcct ctgctcccgg caatgcgccg
    22561 aaatcgcatt gcgccattga tgtaattatc gcagccttac cggagaaaaa cagcggaacg
    22621 gcgatggttt agaaataaaa aaatgcccgg gaatctcatt caccgggcat tgtgtcgttt
    22681 atgcgcagcg cgtgcgctta cttattacac cgcagtctgg aaaatcaccc catcggcttt
    22741 ttcggtgtac tgagaaagct ggttgaagtt cagataacga taagtatcaa cggcagtttt
    22801 atctacttgc gccacgtagg tctggtactc ttccggcgtc ggcagtttgc caatcagcgc
    22861 cgcaacagcc gccagttccg cagaagccag gaagacattc gcgccagtac ccagacggtt
    22921 cgggaagtta cgggtagagg tggaaaccac cgtcgcaccg tctgctacac gcgcctggtt
    22981 acccatacac agggaacagc cagggatctc gatacgcgca ccactcttac cgaagacgct
    23041 gtagtagcct tcttcagtca actgcgcggc gtccatacgg gttggcggtg ccacccacag
    23101 gcgggtcggc agttggcctt tgtgcgcatc cagcagttta cccgcagcac ggaagtgacc
    23161 gatgttggtc atgcaggaac cgataaatac ttcgtcgatc ttctcaccct gtaccgcaga
    23221 cagcggacgc gcgtcatccg ggtcgttcgg tgcacagagg attggctctt taatatccgc
    23281 cagatcgatg tcgatcactg ccgcgtattc agcatctgca tcggcttcca gcagctcagg
    23341 attcgccagc catttttcca tgccctgaat acgacgttcc agggtgcgac gatcgccgta
    23401 accttccgcg atcatccact tcagcaggac gatgttagag ttcaggtatt cgatgatcgg
    23461 ttctttgttc agcttgatgg tacaaccagc ggcagaacgc tcggcggacg catcggtcag
    23521 ttcaaatgcc tgctcaactt tcagatccgg cagaccttca atttccagga tgcggccaga
    23581 gaagatgttt ttcttgcctt tcttctcaac ggtcagcaga ccttgtttga tcgcatacag
    23641 cggaatggca tgtaccagat cgcgcagggt gatgcccggc tgcattttgc ctttgaagcg
    23701 caccagaacg gattccggca tatccagcgg cattacgcct gttgcggctg caaatgccac
    23761 cagaccagaa cccgccggga aagagatacc gatcgggaaa cgggtatggg aatcaccacc
    23821 ggtaccgacg gtatccggca gcagcatacg gttcagccag gagtgaatga cgccgtcacc
    23881 cggacgcagc gacacaccgc cacggttcat aatgaagtcc ggcagcgtgt ggtgcgtgtt
    23941 cacgtcaact ggcttcggat acgccgcggt gtggcagaaa gactgcatca ccaggtcagc
    24001 cgagaagccc aggcacgcca ggtctttcag ttcatcacgg gtcatcgggc cggtggtgtc
    24061 ctgggaacct acagaagtca ttttcggttc acagtacgcg cccggacgaa tgcctttcac
    24121 gccacaggca cggcctacca ttttttgcgc cagcgagaag ccgcgatcgc tctcagcgac
    24181 atctttcgcc tgacggaaca catcactgtg cggcagacca agtgcttcac gcgctttggt
    24241 ggtcaggcca cgcccgataa tcagcggaat acggccacca gcacgcactt catcaatcag
    24301 cacgtcggtt ttcagttcga aggtcgccag cagttcgccg gtttcgtggt tacgcacttc
    24361 acctttgtac gggtaaacgt caatcacgtc gcccatgttc aggttagaga cgtcgacttc
    24421 gattggcagt gcacccgcgt cttccatcgt gttaaagaag atgggtgcaa ttttaccgcc
    24481 gaggcacaaa ccaccgccgc gtttgttcgg cacatgtgga atatcatcgc ccataaacca
    24541 cagaacggag ttagtggcgg atttacgcga agaacccgta cccacaacgt caccgacgta
    24601 cgccagcggg aaacctttct gttgcagagc ttcgatttgc ttgatcggac caacaacacc
    24661 aggctggtct ggctcaatac cttcacgggc gtttttcagc atcgccagcg cgtgcagtgg
    24721 gatatccggg cgtgaccacg catccggtgc cggagaaagg tcatcggtgt tagtttcgcc
    24781 agtgactttg aagacggtaa cggtcagttt ttcagccagc gccgggcgat tcaggaacca
    24841 ttcggcatcc gcccaggact gcataacctg cttcgcatat tcgttgcctg ctttcgcttt
    24901 ctcttctacg tcatagaagt tatcgaacat cagcagcgtg tgagaaagtg ctttggcagc
    24961 aataggtgcc agtttggcat catccagcgc gtcgatcagc ggatgaatgt tgtaaccacc
    25021 ctgcatggtg cccagcagtt cgatggcttt ttccggagtc agcagagggg atttggcttc
    25081 gcctttcgcg atagcagcca ggaagcctgc tttgacatag gcggcttcat cgacgcctgg
    25141 gggaacacgg ttggttaaca gatctaacag gaattcttct tcgcccgcgg gcgggttttt
    25201 cagcagctct acaagtgcgg ccatttggtt tgcatccagg ggtttgggcg caatcccctc
    25261 agcggcacgc tcagctacgt gcttacggta ttcttctagc acgacggttc tcctcgctct
    25321 cattgtcata gtgcggcagg cgattctctt cacgctcctg tgagacagca gtttgtaggg
    25381 taaatgccca gtaccgcaac gggcagaata gcaggatttt gacgatgtgt taatctgttt
    25441 acaaaaaagc aacataaaaa agtggctgaa tcgttaagga tggtctagag attgtttccc
    25501 tccgtaaaaa cttcgttaat tcgcatggtg ataatcacct ttctcaacga atcccaagat
    25561 tagtcaaaat ttaaactacc gcctctttat actcggattc acagcacctg cgggtggcag
    25621 ttcgccgacc attgcgattt ccttgagatc cgaattatga aaatgacttt gccgtttaaa
    25681 ccccatgtgc tggcgctaat ttgcagtgcc gggctttgtg ccgcctctac cgggctatat
    25741 ataaaaagcc gcacagtgga agcgcctgtg gaaccgcaat cgacacaaca gactgcgcct
    25801 gacatcaccg cagttacgct tcctgcaacg gtttccgcac cgcccgtaac gcccgccgtc
    25861 gtcaaatccg cattcagcac tgcacaaatc gatcaatggg ttgcgcctgt cgcgctgtac
    25921 cccgattctc tgctttcaca agtgttaatg gcatcaacct atccggcaaa cgttgctcaa
    25981 gcagtacaat ggtcgcacga taatccactt aaacaaggcg atgctgctat tcaggcggta
    26041 tctgaccagc cgtgggacgc cagcgttaaa tcactggtgg cctttccaca attgatggca
    26101 ttgatgggcg aaaacccgca atgggtgcaa aacctgggcg atgcttttct ggcgcagccg
    26161 caggacgtga tggactcggt acagcgattg cggcaactgg cgcaacaaac cggctcgctg
    26221 aagtcatcaa ccgaacagaa agttattacc acaacgaaga aagctgtacc ggtaacacag
    26281 acagtcacgg ctcccgtcat accatccaat accgtttcaa ctgccagccc cgtcattaca
    26341 gagcctgcaa caaccgtcat ttccattgag cccgccaatc ctgatgtggt ctatattccc
    26401 aactacaacc caaccgtggt ttacgggaac tgggccaata ctgcgtatcc gccggtttat
    26461 ctgccaccac cagccggaga accgtttatt gacagctttg tgcgcggatt cggctatagc
    26521 atgggtgttg ctaccacgta cgcactattc agcagcatcg actgggacga cgacgatcat
    26581 gaccatcatc atcatgacga tgatgattat catcaccacg atggcggtca tcgtgacggt
    26641 aatggctggc aacacaacgg cgacaacatc aatatcgacg tcaacaattt caaccgtatc
    26701 accggtgagc atcttactga taagaatatg gcatggcggc acaatccaaa ctaccgtaat
    26761 ggtgtgccct atcatgatca ggatatggca aagcggtttc atcaaaccga tgtcaacggc
    26821 ggaatgagcg ccacgcagct acctgctcca acacgcgaca gccagcgtca ggcggcagca
    26881 agtcagtttc agcaacgaac acacgccgcc cccgtcatta cacgagatac ccaacgtcag
    26941 gcagcggcac agcggtttaa tgaagctgaa cactatggga gctatgacga cttccgcgac
    27001 ttcagccgtc gccaacccct gacccagcaa caaaaggacg ccgctcgtca gcgttatcag
    27061 tccgcctcgc ctgagcagcg ccaggcggtt cgcgagaaaa tgcagactaa cccacagaac
    27121 cagcagcgaa gagaggcagc gcgtgagcgt attcagtctg caactcccga acagcgccag
    27181 gtatttaagg aaaaagtaca gcagcgccca ctgaaccaac agcaacgtga taacgcccgc
    27241 cagcgtatac aatcggcatc acctgaacaa cgtcaggttt ttcgggaaaa agttcaggag
    27301 agccgcccac aacgtctaaa cgacagtaac catactgtca ggctgaataa cgagcaacgg
    27361 tcggcagtac gcgaacgtct ctctgagcgc ggagcaaggc gacaggaaag gtaaattgca
    27421 gacgtaaaaa aagcggcgtg gttagccgct tttttaattg ccggatgttc cggcaaacga
    27481 acaattactt cttcttcgct ttcgggttcg gcaggtcggt aatgctacct tcgaacactt
    27541 ctgccgccag gcccacagac tcgtgcagag tcgggtgcgc gtggatggtc agtgcgatgt
    27601 cttcagcatc acaacccatt tcgattgcca ggccgatttc acccagcagc tcgccgccgt
    27661 tggtaccgac aatcgcacca ccgatcacac ggtgagattc tttgtcgaaa atcagcttgg
    27721 tcataccgtc tgcgcagtcg gaagcgatag cacgaccaga agcagcccac gggaaggtgg
    27781 cggtttcata gctgatgcct ttctctttcg cttctttctc agtcagacct acccatgcaa
    27841 cttctggttc ggtataggcg atggacggga taactttcgg atcgaagtag tgtttcttac
    27901 cggcgataac ttcagcggca acgtgacctt cgtgaacacc tttgtgtgcc agcatcggct
    27961 gaccgacgat atcgccgata gcaaagatgt gcggtacgtt ggtacgcagc tgtttgtcaa
    28021 cgcggatgaa accacggtcg tccacttcca cgccagcttt gcctgcgtcg aggtttttac
    28081 cgttcggcac acgaccaatc gctaccagca cggcgtcgta acgctgcggt tcagcgggtg
    28141 cttttttgcc ttccatcgtc acataaatac cgtcttcttt cgcttcaacg gcggtaactt
    28201 tggtttccag catcaggttg aatttcttgc tgatacgctt ggtgaagact ttaacgatgt
    28261 ctttgtcagc tgccgggata acctggtcga acatttcaac cacgtcaatc tgtgaaccca
    28321 gcgcgtggta tacggtgccc atttccagac cgatgatacc gccacccatt accagcaggc
    28381 gttctggtac ttctttcagt tccagcgcgt cagtggagtc ccagatacgc ggatcttcat
    28441 gcggaataaa cggcagttgg atcgggcgag aacccgctgc aatgatcgcg ttgtcgaagt
    28501 tgatcacggt tttaccgttc tcaccttcaa cttccagggt gttagccccg gtaaatttac
    28561 ccagaccgtt gaccactttg actttgcggc ctttcgccat accagccaga ccaccggtca
    28621 gctgattgat cactttctct ttccaggtac gaatcttgtc aatatcggtt ttcggttcgc
    28681 cgaagacgat accgtgttca gccagcgctt tggcttcttc gataactttt gctacgtgca
    28741 gcagtgcttt agaagggata cagccgacgt tcaggcaaac accgccaagg gtgttgtaac
    28801 gttctacgat tacggtttcc agacctaaat cagcgcaacg gaaggcagcg gagtaacctg
    28861 cggggcctgc cccaagtacc acgacctgag ttttgatttc agtactcatc atgacctcta
    28921 tatatttatc tccggcggtc atacccgtcg tctttcaggc cgcagcggcg ttagcttcac
    28981 gccctcaccc cagtcactta cttatgtaag ctcctgggga ttctggcgct tgctaccttg
    29041 ctgcacccag aaatccatag ggtatgtgaa gtaataccct aaccaccacc gggtcgttct
    29101 atccgtcggt attttacaaa attgttaaca atttttaaac aacaaacggc aaccgatttg
    29161 tctattcgct aataacctca atacattcat gagattacca gaaaaaagcc ggccgttggg
    29221 ccggctcttt tacttacatc accagacggc gaatgtcaga cagcgtgttg ttaatgatgg
    29281 taatgaaacg ggcaccatca gcaccgtcga tcacgcggtg gtcgaaggag agagaaatcg
    29341 gcagcatcag acgcggcacg aactctttac cattccacac cggctccatc gcggacttgg
    29401 aaacgccgag gatagccact tccggcgcgt tcacaatcgg cgcgaagtgg gtagtaccca
    29461 ggccgccgat gctggagatg gtgaagcaac cgccctgcat ttcgcccgca gtcagcttac
    29521 cgtcacgcgc tttcttagaa atagtcatca gctcgcgaga cagctcgatg atgcctttct
    29581 tgttgacgtc tttgaatacc ggaacaacca gaccgttcgg ggtatccacc gccacaccga
    29641 tgttgatgta tttcttcagg gtcagacgct gaccgtcttc cgacaacgaa ctgttgaagc
    29701 gaggcatctg ctcaagagct gcagcaacgg ctttcatgat gaagacaacc ggggtgatct
    29761 tcacatccag cttacgcttc gccgcttctt cgttctgctg tttacggaac gcttccagct
    29821 cggtgatatc ggttttgtcg aagtgagtaa catgcgggat catcacccag ttacggctca
    29881 ggttcgcacc agaaattttc tggatacggc ccagttccac ttcttcgatt tcaccaaact
    29941 tgctgaagtc caccttcggc cacggcagca tgcctgggat accgccgcca gtcgccgccg
    30001 gagctgcttc tgcacgtttg atagcttctt tcacgtaagc ctgaacgtct tcgcgcagga
    30061 tacgaccttt acggccagtg cccttcactt tcgccaggtt aacgccaaac tcgcgtgcca
    30121 gacggcggat cagcggagtc gcgtgaacgt aagcgtcgtt ttcagcaaat tcagatttgc
    30181 cttcggcttt cgcagccgga gctgctgccg gagcttcagc ttttgctgcc ggagccggcg
    30241 ctgccgcttc ctgtttcgcc ggagccgccg caggcgctgc gccttcaact tcgaagatca
    30301 taatcagcga gccagttttc actttatcgc caacgttgac tttcagttcc ttcacgacgc
    30361 ctgcaaacgg agccggaact tccatagaag ctttgtcgcc ttctacggta atcagtgact
    30421 gttcagcggc aactttgtcg cccactttca ccatcacttc ggtcacttca acttcgtcac
    30481 cgccgatatc cggaacgtta acttctttca cgccagccgc tggtgcaggg gccgctgccg
    30541 gagccgcttc ctgtttagcc gccggagctg ccgcgcctgc ttcacccgcg acttcgaaga
    30601 ccataatcag cgagccggta gacactttgt cacccacgtt cactttgatc tctttcacgg
    30661 tgccagcaaa cggagccgga acttccatag aagctttatc gccttctacg gtgatcagcg
    30721 actgttcagc ttcaacttta tcgccaactt tcaccaggat ttcggtcact tcaacttcgt
    30781 cgctgccgat atccggaacg ttaacgtctt ttgccgccgc agccgcgggt gctgctgccg
    30841 gagctgcttc tttcttctct tctgcctgag caggtgcagc gtctgctgca ccgtcggcgg
    30901 aatcgaaaat cataatcagt gcgccggtct gggttttatc gccaacagag actttgatct
    30961 ctttaacgat acccgcctgc ggagacggaa cttccataga ggctttgtcg ccttctacgg
    31021 tgatcagcga ctgttcggct tcaactttgt cgcccacttt gaccaggatc tcggtgattt
    31081 caacttcatc agccccgatg tccggtactt tgatttcgat agccattatt cttttacctc
    31141 ttacgccaga cgcgggttaa ctttatctgc atcgatgttg aatttggcga ttgcgtcagc
    31201 aaccactttc ttatcgattt cgccacgttt agccagttcg cccagcgccg caaccacgac
    31261 ataagaagca tcaacttcga agtggtgacg caggttctca cggctgtcgg aacgaccgaa
    31321 gccatcagta cccagtacgc ggtagtcgtc agccggtacg taagtacgga cctgctcagc
    31381 gaacagtttc atatagtcgg tagatgccac tgccggagcg tcgttcatca cctgagcgat
    31441 atacggaacg cgcggagttt ccagcggatg aagcatgttc cagcgttcac aatcctgacc
    31501 atcacgcgcc agctcggtga aggaggtcac gctataaacg tcagaaccta cgccgtaatc
    31561 tttcgccagg atctctgccg cttcacggac gtgacgcagg atagaaccgg agcccagcag
    31621 ctgaacttta cctttgctac cttcaatggt ttcgagtttg tagatacctt tacggatacc
    31681 ttcctcagca ccttccggca ttgccggcat gtggtagttt tcgttcagcg tagtgatgta
    31741 gtagtaaacg ttctcttgtt tttcaccgta catacgctcc agaccgtcat gcatgatgac
    31801 agcaacttcg taagcgtaag ccgggtcgta agagatacag ttcgggatag tcagcgactg
    31861 aatgtggctg tgaccatctt cgtgctgcag accttcaccg ttcagggtgg tacgaccgga
    31921 agtaccgccg atcaggaagc cacgcgcttg ctggtcgcca gccgcccagc acagatcgcc
    31981 gatacgctgg aagccgaaca tcgagtaata gatgtagaac gggatcatcg gcagattgtt
    32041 ggtgctgtaa gaggtcgccg ctgccagcca ggaacaacct gcgcccagct cgttgatccc
    32101 ttcctgcaga atctgacctt tctcgtcttc tttatagtaa gcaacctgct cgcggtcctg
    32161 cggggtgtac tgctgaccgt tcgggctgta aataccaatc tgacggaaca gaccttccat
    32221 accgaaagta cgcgcttcgt cggcgatgat cggtaccaga cgatctttga tcgacttgtt
    32281 cttcagcatt acgttcaggg cacgaacgaa agcgatagtg gtagagatct ctttgctctg
    32341 ctcttccagc agcgcgccga agtcttgcag gctcggcagc tcaagcttct cggtgaagtt
    32401 cggctgacgg cttggcagat aaccgtgcag tttctgacgc tgtgcatgca gataggtatg
    32461 ctcttcagaa ccttccggga aggtgatgta cggcagtttt tcgatatctg catcagacac
    32521 cggcacattg aaacggtcgc ggatgtgacg cacgccgtcc atgttcattt tcttaacctg
    32581 gtgcgcgatg tttttacctt cagccgcgtc gcccatgccg taacctttaa tggtatgagc
    32641 aaggattact gtcgctttgc ctttggtttc ctgcgctttc ttgaatgcag cgtagatttt
    32701 cttcggatcg tgaccaccac ggttcagtgc ccagatctgc tcgtcagtcc agtctgcaac
    32761 cagtgctgcg gtttcaggat atttaccgaa gaagtgttca cgaacgtacg caccatcttt
    32821 cgatttgaag gtctggtagt cgccgtcaac ggtttcgttc atcagctgga tcagtttacc
    32881 gctggtatct ttacgcagca gttcatccca acggctaccc cacatcactt tgatcacgtt
    32941 ccagccagca ccttcgaaga tgccttccag ttcgttgatg atcttgccgt taccggtgac
    33001 cgggccgtca agacgctgca ggttacagtt gataacgaag accaggttat ccagtttttc
    33061 acgggtagcg atggtgatcg cacctttgga ttccggttcg tccatttcac cgtcgccgag
    33121 gaacgcgtaa acggtctgtt tagaggtatc tttcaggcca cggtgttcca gatatttcag
    33181 gaatttagcc tggtaaatag caccaatcgg ccccagaccc atagatacgg tcgggaactg
    33241 ccagaattcc ggcatcagtt tcgggtgcgg ataggaagag aggccattgc cgtgaacttc
    33301 ctgacggaag ttatccagct gctcctgagt cagacgacct tccaggaaag cacgagcgta
    33361 cacgcccggg gagatgtggc cctggaagta aaccaggtcg ccgccatcct gctcgttgcg
    33421 tgcacggaag aagtggttaa agcacacatc ataaatggtt gcggaagact ggaaggacgc
    33481 catatggccg cccagttcga ggtctttttt cgacgcacgc agcaccgtca tgatggcgtt
    33541 ccagcggata gctgaacgaa tacggcgttc cagttccaga ttacccggat actccggttg
    33601 ttcttcaacg gggatggtgt tgatgtagtt gctgatacct gtgcctgcgg ctacgtttac
    33661 accgcctttg cgggcttcag caagcagttg gtcgatcaga tactgagcac gctcaacacc
    33721 ttcttcacgg atgaccgatt cgatcgcctg gagccagtcg cgagtttcga tcggatccac
    33781 gtcatttggg aaacgttctg acatgggtta ttccttatct atctaataac gttgagtttt
    33841 ctggaacctg tcccattgaa ctctcgccgg aaagctcaat aagacaggtt ctacgtttag
    33901 ttgccgcgct tttatatgcg cttgatttac aacatcttct ggataatttt taccagaaaa
    33961 atcactaatt ctttcgttgc tccagacgac gcagagaacg ctcacggcgg ctctcttcac
    34021 gacttctgtc gagcaaaatt tcttcgataa aggccagatg gcgatgcgat gcttcgcgcg
    34081 cttcttccgg cttaccggcc ataatcgctt caaatatgcg ggtgcggtga ctactcacca
    34141 gcggcagcat ctcgcgacgc gaatagagca attcgaagtt ctggcggaca ttctgggcca
    34201 acatcggctc catacacctt agcagatgaa gcagaaccac attgtgggcc gcttcggtga
    34261 cggcaatctg atactggagt acggcgtttg attccgcgtc cagatcgcca gactgctgcg
    34321 ccagctctat ggcgtggtgg agttcacgga tgcgttcctt gtcttcatcg gtactacgca
    34381 gcgcggcgta ataagcggcg ataccttcca gggcgtgtcg tgtttcgagc aagtcatact
    34441 gtgactcagg atggtcggag agcagctcca ccagcggatc gctgaagctt tgccataggc
    34501 tgctctggac aaaagtgccg ccaccctggc gacgaagcaa caagcccttc gcttcgagac
    34561 gttgaatcgc ctcacgcaag gagggacggg agacgtcaaa ctgttttgcc agttcgcgtt
    34621 ccggtgggag tttttcgccc gggcggagag tgccttcgag gatcaaaaac tccagttgct
    34681 gctcaatcac atcggagagt tttggttggc ggattttgct gtaggccatg agttcctgtc
    34741 ttaagccact tgccgaagtc aattggtctt accaatttca tgtctgtgac gctaaagtaa
    34801 caaagtattc accttatgtc catacaggtt ttgattgaaa tcatgaaact gtgcacattt
    34861 taacaacttg acatatataa cgtttcaaag ttgtaactat gcacaaatgt agactttacg
    34921 taggaaagga gttttttaac gattattaaa ctataaaaat ccgaattgaa ccgattcact
    34981 taccaatttt gtgattttta attcaattaa aacgaattta aattcattct acatattgag
    35041 aggggttgag gctgagcttt acaaacggtt tctttttaag caactcatct tcaaccatgc
    35101 ataaagtggg tgcattcgct gccgcatacc attattcttg atctgacgga agtctttttg
    35161 taacaattca aacttctttg atgtaaacaa attaatacaa caaacggaat tgcaaactta
    35221 cacacgcatc actgcgtaga tcaaaaaaac aaccaccgca cgaggtttca tgatggaagg
    35281 tcaacagcac ggcgagcagc taaagcgcgg ccttaaaaac cgccatattc agcttatcgc
    35341 gctgggtggc gcgataggga ccgggttatt cctgggtagc gcctccgtaa tacagtctgc
    35401 agggccaggg attatcctgg gttacgccat tgctggtttt atcgcctttc tgatcatgcg
    35461 tcagctgggt gaaatggtgg tcgaagaacc tgtcgcaggc tcctttagcc actttgctta
    35521 taaatactgg ggcagttttg ccggtttcgc ctctggctgg aactactggg tactgtacgt
    35581 tttagttgcc atggctgagc tgactgccgt gggtaaatac attcagttct ggtatccgga
    35641 aatccccacc tgggtttctg ccgccgtatt ctttgtggtg attaacgcca tcaacctgac
    35701 caacgttaaa gtgtttggcg agatggagtt ctggtttgcc attatcaaag ttatcgcggt
    35761 ggtagcgatg atcatcttcg gcggctggct gctattcagt ggcaacggcg gcccgcaggc
    35821 gaccgttagc aacctgtggg atcagggtgg tttcctgccg cacggcttca ccgggctggt
    35881 gatgatgatg gcgattatca tgttctcgtt cggtggtctg gaactggtgg ggatcaccgc
    35941 agcagaagct gataacccgg agcaaagtat accgaaagca actaaccagg ttatctaccg
    36001 catcctgatt ttctatattg gttcgttagc cgttctgctc tcactgatgc cgtggacccg
    36061 cgttaccgcc gataccagtc cgtttgtgct gatcttccac gagttaggcg atacctttgt
    36121 ggcgaatgcg ctgaacatcg tggtactgac tgcggcgctc tccgtgtaca acagctgcgt
    36181 atattgcaac agccgtatgc tgtttggtct ggcacaacag ggtaatgcgc caaaagcgct
    36241 ggcgtctgtc gataaacgtg gtgtaccagt aaataccatt ctggtgtctg cactggtaac
    36301 ggcgttgtgc gtactgatta actaccttgc cccagagtcc gctttcggac tgttaatggc
    36361 gctggtggta tctgcactgg taatcaactg ggcgatgatt agcctggcgc atatgaaatt
    36421 ccgtcgcgcc aagcaggaac aaggcgtggt aactcacttc cctgctctgc tttatccgct
    36481 gggtaactgg atctgcctgc tgtttatggc ggcggtactg gtgattatgc tgatgacccc
    36541 aggaatggcg atttcggtat acctgatccc ggtatggctg atcgtgttag gtatcggcta
    36601 tctgtttaaa gagaaaaccg ccaaagccgt aaaagcgcat taatctctct acgccctcac
    36661 ccgtacaggg tgagggcaat aatctttaca ccaacgcccc gtaaatcgtc agtagtgcaa
    36721 tcaccaccac gaccacgaac gaggttttct tcgccattga aaccgctgcc ctcggcgtct
    36781 ccaccttatc gacatgcggt tcacgcgcca gagagaactg cgccagacgc gttaacacct
    36841 gatactgcga agtatggaaa tcacccagcg aagcaaacca ggccggtaac gctttctcac
    36901 catgaccgat caaggcatat accacacccg caagacgaac cggcacccaa tccagtacat
    36961 gaagcacggc atcaatgccg gactgtaaac gatgatgcgg cgtctgatat cgcgccagcc
    37021 agtattgcca tgcacgcaaa aacgcatacc ccatcagcgt aacgggtccc caggttcccc
    37081 ccacaatcag ccagaacagc ggtgcaagat aaaaacgaaa gttaatccac agcaatgcat
    37141 tttgcagctc acgcaaatac tcacgttcgt cgcagcctgc cgggacgccg tgaatcatgg
    37201 tgagttcgcc agccatcgtg gcacgggcat ggctatcatt acgtgaagca gctgtcagat
    37261 aagcatgata atgaagacga actttacctg cgccaataca cagcaaacca atcagcagcc
    37321 acaccagtag cgtgggaacg ttgaacaata ctccctgcaa tgcgcgtaac agtaaaaaag
    37381 tcacgcccat cgcaataatg gtcatgccta acgtgcgccc gagagaaaaa tgtttcaccc
    37441 gccgaaagaa cgcttcaaga cgatgatcaa gctgccagtg ctcgcccaac ttaaacaggc
    37501 gctcgaaaat taacaccagt aaggttgtaa atagcgtcat gttgtctcct tgctgaccag
    37561 cacacgaaac cgtgcccaat caaatgcagg accgggatcg gttttccgat ccggcgcaat
    37621 atcacaatgg cccgtcatgt ttttagcgat atccggatag caatcaatca gtgcccgcgt
    37681 aaccgccgca agctgttgat actgcgcatc ggtatacgcc agcgtatcgg tgccttcaag
    37741 ctcaatccca atagaaaaat cattgcagcg ttcgcgcccc tgatactgag agactcccgc
    37801 atgccatgca cgtttatcga aaggaacata ctggactatt tcaccatcac ggcgaatcaa
    37861 acagtgagcg gagacgcgca aatgggcgat ctcagcaaag aaaggatgtg cctgcggatc
    37921 aatagttcca gtgaataatg cgtcgatcca cggaccgcca aactcgcctg gcggcaggct
    37981 aatattgtgc accaccagca gggtgggtgt ttcgtcatcc gggcggcaat cgtaatgtgg
    38041 tgagggaacg cggcgcgcgc caaccagcca cccctgttct aacaacatgc aggatctcct
    38101 tatatgtggt gctaataccc ggttcagagt agcatgtttc taccttatga ttcgttagct
    38161 atctggagtt ttaacatgcc gcctcgccgc tataaccctg acacccgacg tgacgagctg
    38221 ctggaacgca ttaatctcga tatccccggc gcggtagccc aggcgctgcg ggaagattta
    38281 ggcggaacag tcgatgccaa caatgatatt acggcaaaac ttttaccgga aaattctcgc
    38341 tctcatgcca cggtgatcac ccgcgagaat ggcgtctttt gcggcaaacg ctgggttgaa
    38401 gaggtgttta ttcaactggc aggcgacgat gtcaccataa tctggcatgt ggatgacggc
    38461 gatgtcatca atgccaatca atccttgttc gaacttgaag gcccatcccg cgtgctgtta
    38521 acgggcgaac gcactgcgct taattttgtg caaacccttt caggagttgc cagtaaggta
    38581 cgccactatg tcgaattgct ggaaggcacc aacacgcagt tgttggatac gcgcaaaacc
    38641 ttacccggcc tgcgttcagc tctgaaatac gcggtacttt gcggcggcgg agcgaatcac
    38701 cgtctggggc tttctgatgc cttcctgatc aaagaaaacc atattattgc ctccggctca
    38761 gtgcgccagg cggtcgaaaa agcgtcctgg ctgcacccgg atgcgccagt agaagtcgaa
    38821 gtagagaatc tggaagaact tgatgaagcc ctgaaagcag gagccgatat catcatgctg
    38881 gataacttcg aaacagaaca gatgcgcgaa gccgtcaaac gcaccaacgg caaggcgcta
    38941 ctggaagtgt ctggcaacgt cactgacaaa acactgcgtg aatttgccga aacgggcgtg
    39001 gactttatct ccgtcggtgc gctaactaaa cacgtacaag cactcgacct ttcaatgcgt
    39061 tttcgctaat agttgacaga tttatccgct ccatcgcggg cggataatct ccccttcccc
    39121 aactttcttc gtaacgcctc gcaaatttct tgtctttcgc tgcaacgaaa taaaaaacct
    39181 cggaatctgc tttcccgttt cgttttttgc ccctcgcctg tgctgatggc gtttgccaaa
    39241 gtagcgccaa ctaaatcaag gagcgaaaca gatggacaag caacgcggtt ttacacttat
    39301 cgaactgatg gtggttattg gcatcattgc cattttaagc gccattggta ttcccgctta
    39361 tcaaaactac ctgcgcaaag ccgcactcac cgacatgcta caaacctttg tgccttaccg
    39421 taccgccgta gagttgtgcg cgctggaaca tggtggatta gatacctgcg acggtggcag
    39481 caatggcatt ccctcgccta ccaccacccg ctatgtttca gccatgagtg tggcaaaggg
    39541 cgtggtgtcg ctgaccgggc aagaaagtct caatgggcta agcgtcgtca tgacaccggg
    39601 ttgggataac gcaaacggcg tcaccggctg gacgcgcaac tgcaatattc aaagtgacag
    39661 cgcattgcat caagcctgcg aagatgtctt ccgctttgat gacgccaact aaggagcggc
    39721 aatgaatatt ccacagctca ctgccctgtg tctgcgttat catggagtct tgctggatgc
    39781 cagcgaagag gtggttcatg ttgcggtagt cgatgcacct tcgcatgagc tactggacgc
    39841 attgcatttc gctaccacca aacgtattga gatcacctgc tggacgcgcc aacaaatgga
    39901 aggtcacgcc agtcgcacac aacagacatt gcccgtagct gttcaggaga agcatcagcc
    39961 caaagcagag ttgctaactc gaacgttaca atctgcgctg gaacaacgcg cgtctgatat
    40021 tcatatcgaa ccagcggaca atgcctaccg catccgcttg cgtatcgacg gcgtattgca
    40081 tcctttaccg gatgtttcac cggatgccgg agtcgcatta accgccagat taaaagtgct
    40141 gggaaacctg gatattgcgg aacatcgcct gccgcaggac gggcaattca ctgtcgaact
    40201 ggcaggaaac gccgtctcat ttcgtattgc gaccttacca tgtcggggtg gtgaaaaggt
    40261 ggtattaagg ttgttacagc aggtgagcca ggcactggat gtcaacacgc ttggaatgca
    40321 gccgttacaa ctggcggact ttgctcatgc cttgcaacaa ccacagggac tggtgctggt
    40381 aactggccct acaggcagcg gcaaaacggt cacgctttat agtgccctgc aaacgctgaa
    40441 taccgctgac attaatattt gtagcgtcga agatccggtt gagatcccca tagccggact
    40501 aaaccagacg caaatccatc cgcgtgccgg actcaccttt cagggcgtgt tgcgtgcgtt
    40561 attgcgccag gatcctgacg tcatcatgat cggagagatc cgcgatggcg aaacagcaga
    40621 gatcgctatt aaagcggcgc aaactggtca cctggtgttg tctaccctac acactaattc
    40681 cacctgcgaa acgctggtac gtttacagca aatgggggtc gcccgctgga tgctatcatc
    40741 ggcgcttacg ctggtaatag cccagcgtct ggtacgcaaa ctttgcccac attgtcgccg
    40801 gcagcaaggg gagcccatcc acattccaga caatgtatgg ccatcgccgc tgccccactg
    40861 gcaggcaccc ggttgtgtac attgctacca cggtttttat ggtcgtacgg ccttatttga
    40921 agttctgccc ataacgccgg tcattcgtca gcttatttcc gctaataccg acgttgaatc
    40981 gctggaaacg cacgcacgac aggcgggtat gcgtacgctt tttgaaaacg gctgcctggc
    41041 cgtggagcaa ggcttaacca cctttgaaga gttaatccgc gtactgggga tgccgcatgg
    41101 cgagtaagca actctggcgc tggcatggca taaccggcga cggcaatgcg caagatggga
    41161 tgctatgggc agagagccgt gctttgctgc tcatggcact acagcaacag atggtttgta
    41221 accaggtgaa tcatttgata ccgacaaggg ttttagtgaa aggaggtgaa atcacctgta
    41281 agatgctgaa ataaaacagg tgatgtatac ttcggacgga aaactctttt tgataccgac
    41341 tgctcaacaa atagtcaaaa gatcaaaaaa cggcgacaca tttgatactg ctaatttacc
    41401 cttaaaatct tcttcaaaac atcttcaaac tgccaaaatg ctcacataat cgatctaact
    41461 ataatcagta tgataatcat catgaattac tattcaatag cgtgattatc ttgggatgag
    41521 tcatatgatc tctatatgta tttacagcac cacctcatga tagcgatggc gcttaaccaa
    41581 caacattttt ctttactgtc actgcccctg attcctgggt ggcgttattg ctcgagttgt
    41641 acttctcaat aacgcgcaaa atctctttgt ctgtttcttc acttcccgcc attcctatct
    41701 ttattgcatt ttttagtacg ggacgtaatg gcaaggcatc aatgatgctt tctaccgcat
    41761 cctggctgat tgtagatgta ttcattgctg ccagcctgac aaggttgtta gtaccttctc
    41821 gcctgatgaa tgtaaggatt aattgcttct cttgggggct tagagagcta agcgcctgat
    41881 caatacccga acctgaatcg caattttttt cgatttttcc gcattcatct tcgttcttgc
    41941 ctgtggccaa ccattcgagt gaacaattac aggctttagc tattaaggcc agtttatcca
    42001 aaggagggaa gtttttcccc ttcctgtaat ttcttactgt ggcttcagat agtccacaaa
    42061 gggtcgccag ttcgacgttt gtcatgtttc ccatagccat agctaagcgt tctgaaaatc
    42121 ggtttattcc ttccatatca ataaaccgtt ccgcactgga tacggttttt tctgccttat
    42181 ccatataacc ccctataaaa caaagaaatt atcggcatcg catttaattt cgatatgctc
    42241 gcaaaaaacc gttgatatcg aattattttg cgattatgat ttacctaaat cgataaccgg
    42301 ttttgattat cggttcaggt aaacatgaga ggatcacaca atgagggtat ccagacaagc
    42361 atcgaataat ttgtttgttc gagaggggtg tgactggtct gctaaagcgg tatatgcagc
    42421 ccttgaaaac aaaggcacag acctccctga actggaaagg gagttaggat taaaaagcgg
    42481 atcaatgcgc aatgtgtttt accgccagtg ccgtaaatac gaagccgcca tcgccgaaaa
    42541 aataggggtt gaacctgctg taatctggcc cagcagatac caggtggacg ccagagaagt
    42601 ggcttgatgg gggatgtatg tgggtaacag caaaagaatg tgttggtctt cctgatttac
    42661 caatgatgga acataacatt cgccatcgac tcacaaaagc agctgcggga aatgcagcca
    42721 tgttacgcaa acgtaagggg acaaaagcaa tcgaatttaa tatcgattgt ttgccttctg
    42781 gcgcgcaaca agtattgcgt gaacgttatg cccttcaact gatgacgcaa aaagctgatg
    42841 aatcaccggc tccggtggtg acaaaggcca gacgctcatc tgccgtggtt gatgcggtgg
    42901 aggcatatcg cggatcacca caactgatgc tcgaacgcct caatgccctg actgaaaacc
    42961 agcgccaggt ggctgatgca cgaatcgcga tcgtcagcga agttctgaaa gtcgcgcaac
    43021 aacctggttt cagctgtgcg aaggctatcc ggtttatcgt tgacaacctg gcacgttcgc
    43081 aactggacga gcgcattgtg gcaatggttg agacggcgaa cgccaaaaag ggaaacagcc
    43141 gtgcgttgag tgaaatcacg ctgaaacgct ggattgcagc ctttaacaag gcgcagaacg
    43201 ccgctgaacg cctgcttttg ctggcacctg gtaagcgcca ggaaataaaa gccgaagata
    43261 ttaactggct gcccgaattt ctggcgcagt atcgccagtc aaacggccga ccaatgaccg
    43321 aggcttacga ggattttgtc gctgaatggc agcaccggca cgctgatgag ccttatatgc
    43381 tcgatatcat gccgtcttat gacaccattc gccgcgcaat gaagaaactg ccggaagtgg
    43441 tgaaacaaaa aggccgggtg accggcagtg aataccgcca gcttgaggga ttcacgcgcc
    43501 gcgactggtc cagaatgccg gtgaactatg tctggattgg tgacggtcac ggcatgaagc
    43561 tgaaatgcag gcacccggtt cacgggcggc catttgcacc ggaagtaacc tttgttatcg
    43621 atggcggtac acgctttgtg gtgggctgga gccttgacct ggctgaaaat gttttcgccg
    43681 tagccggtgc catacagcac ggtattcgac atcacggcaa accgtttctg tattactcgg
    43741 ataatggctc cggggaaac
//
LOCUS       DAZFQD010000022        43470 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.022, whole genome shotgun sequence.
ACCESSION   DAZFQD010000022 DAZFQD010000000
VERSION     DAZFQD010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 43470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43470
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.022"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..1094
                     /locus_tag="ACHKTW_001315"
                     /pseudo
     CDS             1..1094
                     /locus_tag="ACHKTW_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312389.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
     gene            1356..2978
                     /locus_tag="ACHKTW_001316"
     CDS             1356..2978
                     /locus_tag="ACHKTW_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HFO6462374.1"
                     /translation="MKIGTVAGTNDSTTTIATNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVVEKIDDWQIERFQQSIRDDKISFTIQTNHSEKYSMLSDMRAHILRR
                     DNNYQFIVTINSKNYGCYLDNTDINWCSIVYLLNNMTVNDDANDVAVTESYKPVWDWK
                     ISQFNVSDIKFETMINPQFADRTYFSNCSPVDPTSTRPTYFGDTDGSVGAVLYALFAT
                     GHLRIMAEGENFLSQLLNIEDEVLNVLLRENFNEQLDTNVNTIISILNRRDIVLESLQ
                     PYLVINKDAVTPCTFLGDQTGDRFSNICGDQFIIDLLKRIMSINENVHVLAGNHETNC
                     NGNYMQNFTRMKPLDEDTYAGIKDYPVCFYDPKYKIMANHHGITFDEQRKRYIIGPIT
                     VSIDEMTNALDPVELAAIINKKHHAIINGKKFKTSRAISCRSFNRYFSVSTDYRPKLE
                     ALLACSQMLGINQVVAHNGNGGRERIGETGTVLGLNARDSKHAGRMFSMHNCQINPGA
                     GPEITTPWKSYQHEKNRNGLMPLIRRRTMLQL"
     gene            3168..3281
                     /gene="yhiY"
                     /locus_tag="ACHKTW_001317"
     CDS             3168..3281
                     /gene="yhiY"
                     /locus_tag="ACHKTW_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhiY"
                     /protein_id="HFO6462375.1"
                     /translation="MMTTLLPVFTKPSPLALNALRAGRICRFLLIPDGRIR"
     gene            3344..4411
                     /gene="yhiI"
                     /locus_tag="ACHKTW_001318"
     CDS             3344..4411
                     /gene="yhiI"
                     /locus_tag="ACHKTW_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFO6462376.1"
                     /translation="MDKSKRHLAWWVVGLLAVAAIVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHTRSRSLAHRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAELGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            4408..7143
                     /gene="rbbA"
                     /locus_tag="ACHKTW_001319"
     CDS             4408..7143
                     /gene="rbbA"
                     /locus_tag="ACHKTW_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HFO6462377.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVMVLGGDMRDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVIHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIVRGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            7143..8267
                     /gene="yhhJ"
                     /locus_tag="ACHKTW_001320"
     CDS             7143..8267
                     /gene="yhhJ"
                     /locus_tag="ACHKTW_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO6462378.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQKIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKIWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            8371..8601
                     /locus_tag="ACHKTW_001321"
     CDS             8371..8601
                     /locus_tag="ACHKTW_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HFO6462379.1"
                     /translation="MDQLFKSPLPQGIKWTDIESLVKALGGEIKEGRGSRCKFILNMS
                     VACFHRPHPSPDTDKGAVESVRDWLLSIGVKP"
     gene            8598..8957
                     /locus_tag="ACHKTW_001322"
     CDS             8598..8957
                     /locus_tag="ACHKTW_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFO6462380.1"
                     /translation="MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSD
                     SIQGLQKEGELSLREYLEDCKAAGIEPYVRTEKIKTFTLRYPESLSERLNNAAAQQQV
                     SVNTYIIETLNERLNHL"
     gene            complement(9077..9478)
                     /gene="nikR"
                     /locus_tag="ACHKTW_001323"
     CDS             complement(9077..9478)
                     /gene="nikR"
                     /locus_tag="ACHKTW_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HFO6462381.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(9484..10290)
                     /gene="nikE"
                     /locus_tag="ACHKTW_001324"
     CDS             complement(9484..10290)
                     /gene="nikE"
                     /locus_tag="ACHKTW_001324"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HFO6462382.1"
                     /translation="MTLLNVCGLSHHYAHGGFSGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPSQGNISWRGEPLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQRV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGSACLFITHDLRLVERF
                     CQRVMVMDNGQIVETQVVGDKLTFSSDAGRVLQNAVLPAFPVRRRTTEKV"
     gene            complement(10287..11051)
                     /gene="nikD"
                     /locus_tag="ACHKTW_001325"
     CDS             complement(10287..11051)
                     /gene="nikD"
                     /locus_tag="ACHKTW_001325"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HFO6462383.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKPVSPCALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGK
                     IVEQGDVETLFNAPKHTVTRSLVSAHLALYGMELAS"
     gene            complement(11051..11884)
                     /gene="nikC"
                     /locus_tag="ACHKTW_001326"
     CDS             complement(11051..11884)
                     /gene="nikC"
                     /locus_tag="ACHKTW_001326"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015099 - nickel cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HFO6462384.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWLPYDPQAIDLPSR
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            complement(11881..12825)
                     /gene="nikB"
                     /locus_tag="ACHKTW_001327"
     CDS             complement(11881..12825)
                     /gene="nikB"
                     /locus_tag="ACHKTW_001327"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HFO6462385.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGGWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            complement(12825..14399)
                     /gene="nikA"
                     /locus_tag="ACHKTW_001328"
     CDS             complement(12825..14399)
                     /gene="nikA"
                     /locus_tag="ACHKTW_001328"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO6462386.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALSKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWILQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKALLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            complement(14510..15097)
                     /gene="acpT"
                     /locus_tag="ACHKTW_001329"
     CDS             complement(14510..15097)
                     /gene="acpT"
                     /locus_tag="ACHKTW_001329"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HFO6462387.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            complement(15152..16201)
                     /gene="yhhT"
                     /locus_tag="ACHKTW_001330"
     CDS             complement(15152..16201)
                     /gene="yhhT"
                     /locus_tag="ACHKTW_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFO6462388.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLELM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGIYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            16333..17550
                     /gene="yhhS"
                     /locus_tag="ACHKTW_001331"
     CDS             16333..17550
                     /gene="yhhS"
                     /locus_tag="ACHKTW_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO6462389.1"
                     /translation="MPEPVAEPALNGLRLNLRIVSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVTY
                     GAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASKGKPLPFRAVLG
                     SVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMPWMAKIGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVAIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            complement(17554..18111)
                     /gene="dcrB"
                     /locus_tag="ACHKTW_001332"
     CDS             complement(17554..18111)
                     /gene="dcrB"
                     /locus_tag="ACHKTW_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HFO6462390.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(18184..18849)
                     /gene="yhhQ"
                     /locus_tag="ACHKTW_001333"
     CDS             complement(18184..18849)
                     /gene="yhhQ"
                     /locus_tag="ACHKTW_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HFO6462391.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSIFGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            19070..19315
                     /gene="tusA"
                     /locus_tag="ACHKTW_001334"
     CDS             19070..19315
                     /gene="tusA"
                     /locus_tag="ACHKTW_001334"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097163 - sulfur carrier activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HFO6462392.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            complement(19417..21615)
                     /gene="zntA"
                     /locus_tag="ACHKTW_001335"
     CDS             complement(19417..21615)
                     /gene="zntA"
                     /locus_tag="ACHKTW_001335"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HFO6462393.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSSPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQATDEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAELAIPTAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGIIALQDTLRADAA
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTKLNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(21689..22315)
                     /gene="yhhN"
                     /locus_tag="ACHKTW_001336"
     CDS             complement(21689..22315)
                     /gene="yhhN"
                     /locus_tag="ACHKTW_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HFO6462394.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYL"
     gene            22456..22815
                     /gene="yhhM"
                     /locus_tag="ACHKTW_001337"
     CDS             22456..22815
                     /gene="yhhM"
                     /locus_tag="ACHKTW_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HFO6462395.1"
                     /translation="MSKPPLFFIVIICLIVVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFSLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            complement(22818..23087)
                     /gene="yhhL"
                     /locus_tag="ACHKTW_001338"
     CDS             complement(22818..23087)
                     /gene="yhhL"
                     /locus_tag="ACHKTW_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HFO6462396.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(23077..23673)
                     /gene="rsmD"
                     /locus_tag="ACHKTW_001339"
     CDS             complement(23077..23673)
                     /gene="rsmD"
                     /locus_tag="ACHKTW_001339"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HFO6462397.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRAVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQREAQGESDAD"
     gene            23823..25319
                     /gene="ftsY"
                     /locus_tag="ACHKTW_001340"
     CDS             23823..25319
                     /gene="ftsY"
                     /locus_tag="ACHKTW_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFO6462398.1"
                     /translation="MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     VKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVAESEKAQPEAEVVAQPEPVAEETP
                     EPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEM
                     GEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            25322..25990
                     /gene="ftsE"
                     /locus_tag="ACHKTW_001341"
     CDS             25322..25990
                     /gene="ftsE"
                     /locus_tag="ACHKTW_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFO6462399.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            25983..27041
                     /gene="ftsX"
                     /locus_tag="ACHKTW_001342"
     CDS             25983..27041
                     /gene="ftsX"
                     /locus_tag="ACHKTW_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFO6462400.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            27286..28140
                     /gene="rpoH"
                     /locus_tag="ACHKTW_001343"
     CDS             27286..28140
                     /gene="rpoH"
                     /locus_tag="ACHKTW_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HFO6462401.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMQGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            28411..29514
                     /gene="livJ"
                     /locus_tag="ACHKTW_001344"
     CDS             28411..29514
                     /gene="livJ"
                     /locus_tag="ACHKTW_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HFO6462402.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(29713..30096)
                     /gene="panM"
                     /locus_tag="ACHKTW_001345"
     CDS             complement(29713..30096)
                     /gene="panM"
                     /locus_tag="ACHKTW_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0031638 - zymogen activation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HFO6462403.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPAVSCWWMADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
     gene            30520..31629
                     /gene="livK"
                     /locus_tag="ACHKTW_001346"
     CDS             30520..31629
                     /gene="livK"
                     /locus_tag="ACHKTW_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HFO6462404.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINAKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            31677..32603
                     /gene="livH"
                     /locus_tag="ACHKTW_001347"
     CDS             31677..32603
                     /gene="livH"
                     /locus_tag="ACHKTW_001347"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HFO6462405.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTVFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYGVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            32600..33877
                     /gene="livM"
                     /locus_tag="ACHKTW_001348"
     CDS             32600..33877
                     /gene="livM"
                     /locus_tag="ACHKTW_001348"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HFO6462406.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDTASDIR
                     WQWVFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLAVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTLFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAILLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            33874..34641
                     /gene="livG"
                     /locus_tag="ACHKTW_001349"
     CDS             33874..34641
                     /gene="livG"
                     /locus_tag="ACHKTW_001349"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HFO6462407.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            34643..35359
                     /gene="livF"
                     /locus_tag="ACHKTW_001350"
     CDS             34643..35359
                     /gene="livF"
                     /locus_tag="ACHKTW_001350"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HFO6462408.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGGQ"
     gene            35752..37068
                     /gene="ugpB"
                     /locus_tag="ACHKTW_001351"
     CDS             35752..37068
                     /gene="ugpB"
                     /locus_tag="ACHKTW_001351"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HFO6462409.1"
                     /translation="MKPLHYTASALALGLALMGNAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAAFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTPVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYAAKLKASGMKCGYASGWQGWIQLENFSAWNGLPFASKNNGFDG
                     TDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAN
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKNKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADTATRQMLNKPPLPFTKGLRLGN
                     MPQIRVIVDEELESVWTGKKTPQQALDTAVERGNQLLRRFEKSTKS"
     gene            37166..38053
                     /gene="ugpA"
                     /locus_tag="ACHKTW_001352"
     CDS             37166..38053
                     /gene="ugpA"
                     /locus_tag="ACHKTW_001352"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HFO6462410.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSQFVGLDNFVALFHDSYYLDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALQSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSVVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            38050..38895
                     /gene="ugpE"
                     /locus_tag="ACHKTW_001353"
     CDS             38050..38895
                     /gene="ugpE"
                     /locus_tag="ACHKTW_001353"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HFO6462411.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLQMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            38897..39967
                     /gene="ugpC"
                     /locus_tag="ACHKTW_001354"
     CDS             38897..39967
                     /gene="ugpC"
                     /locus_tag="ACHKTW_001354"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol 3-phosphate ABC transporter ATP
                     binding protein UgpC"
                     /protein_id="HFO6462412.1"
                     /translation="MAGLKLQAVTKSWDGKTQVIKPLTLDVADGEFIVMVGPSGCGKS
                     TLLRMVAGLERVTEGDIWINDQRVTEMEPKDRGIAMVFQNYALYPHMSVEENMAWGLK
                     IRGMGKQQIAERVKEAARILELDGLLKRRPRELSGGQRQRVAMGRAIVRDPAVFLFDE
                     PLSNLDAKLRVQMRLELQQLHRRLKTTSLYVTHDQVEAMTLAQRVMVMNGGVAEQIGT
                     PVEVYEKPASLFVASFIGSPAMNLLTGRVNNEGTHFELDGGIELPLNGGYRQYAGRKM
                     TLGIRPEHIALSSQAEGGVPLVMDTLEILGADNLAHGRWGEQKLVVRLAHQERPTAGS
                     TLWLHLAENQLHLFDGETGQRV"
     gene            39964..40707
                     /gene="ugpQ"
                     /locus_tag="ACHKTW_001355"
     CDS             39964..40707
                     /gene="ugpQ"
                     /locus_tag="ACHKTW_001355"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFO6462413.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGESLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARQLWAGMTPPLLSSFEIDALEAAQLAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKVRVMQLKDAGLRILVYTVNKP
                     QRAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(40694..41134)
                     /gene="yhhA"
                     /locus_tag="ACHKTW_001356"
     CDS             complement(40694..41134)
                     /gene="yhhA"
                     /locus_tag="ACHKTW_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HFO6462414.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPSTPQPDIPLKTIGP"
     gene            41254..42996
                     /gene="ggt"
                     /locus_tag="ACHKTW_001357"
     CDS             41254..42996
                     /gene="ggt"
                     /locus_tag="ACHKTW_001357"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036374 - glutathione hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HFO6462415.1"
                     /translation="MIKPTFLRRVAIAALLSGSCFSAAAAPPAPPVSYGVEEDVFHPV
                     RAKQGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIR
                     SKNGNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALDK
                     YGTMPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLKK
                     GDTLVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVERT
                     PISGDYRGYQVYSMPPPSSGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYAY
                     ADRSEYLGDPDFVKVPWQALTNKAYAKSIADQIDINKAKPSSEIRPGKLAPYESNQTT
                     HYSVVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNVYGLVGGD
                     ANAVGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTVLQMVVNSIDYGMNVAEA
                     TNAPRFHHQWLPDELRVEKGFSPDTLKLLEAKGQKVALKEAMGSTQSIMVGPDGELYG
                     ASDPRSVDDLTAGY"
     gene            complement(43034..43318)
                     /gene="yrhB"
                     /locus_tag="ACHKTW_001358"
     CDS             complement(43034..43318)
                     /gene="yrhB"
                     /locus_tag="ACHKTW_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhB family protein"
                     /protein_id="HFO6462416.1"
                     /translation="MITYHDAFAKANHYLDDADLPVVITLHGRFSQGWYFCFEAREFL
                     ETGDEAARLAGNAPFIIDKDSGEIHSLGTAKPLEEYLQDYEIKKATFGLP"
BASE COUNT        10344 a        11007 c        11975 g        10144 t
ORIGIN      
        1 ggtaatttca aacctgactg gcgttgggat gtctctaaag agacgtttac taaggaaaag
       61 tttgatagct atgttaaacc tgtgttttca aaaatagact tctataaaca gtgtggtgtg
      121 attaacccac aaaatgccaa cacggcttat tttggcgata cagatggaag agtaggcgct
      181 gtactttatg cattgctcgt ttcagggcat ataggcatca gggaaaaggg ttggagttta
      241 ttatgtgaat tattaaaaca cgaagagatg gcttcatctg catacaaaca taaaaataat
      301 aaagtgctct acgatttatt aaatacgaga gacatgatct tgaatgagtt gcatcaacat
      361 gtttttttaa aggacgatgc gataacccct tgtatcttcc ttggggatca tacaggcgat
      421 agatttagta ctatttttgg tgataaatat atccttactt tgctaaattc tatgcgaaat
      481 atggagggta ataaagatag ccgtattaat aaaaatgtcg tcgttctggc tggaaatcat
      541 gaaataaatt ttaatggtaa ctatactgca cgactagcaa atcataaact ctctgccggt
      601 gatacgtata acttaattaa aacgctcgat gtttgtaatt atgactcaga aaggcaagtt
      661 ttaaccagtc atcacggaat aataagagat gaagaaaaaa atgttattgt ctcggtgctt
      721 tgcaggttcc atttaatcag atgaaaaatc ctactgatcc tgaggaatta gccaatattt
      781 ttaataaaaa acataaagaa cacatggatg atcgttttat ccatctaatt agaagcaatg
      841 ctatagcatc cacacctgta tatgacaatt attttaataa cactactgct ttcagaccaa
      901 aacctgaaga tatcttcaaa tgtggacaga cgctcaaaaa aactaaacag aaatatggcc
      961 attatggatt aggggtagat cagcatcaaa aaattgataa ttataccatg gggctaaatt
     1021 cctggaagat agctcctaac gaacgtggtg ataaaaaagg tgtcccaggg cttagttgtt
     1081 ttcaaccaca ttaaaactcc atgagtgctg tggtatatct ttttaatcct gccagtattt
     1141 cataggtttt cttaatcgat tgtagaggca tgttctgatg acgcctctac aatctttagg
     1201 gactttctct gctatgagat aagaaatttc atattatgct ttaaggctga atgatttgtg
     1261 caaaatataa ttatttttcc atcgaacatg aggtgtatgt cataagatta aatgttaact
     1321 atgcattcct cgcggtgaat tatatgaggt aaaagatgaa aattggaact gtggcaggca
     1381 ctaacgacag tacaacgaca atcgcaacaa atgatatggt gcaagagcat gttactaact
     1441 tcactaaaga actgtttggg tatattgcaa atggtatagg tgatgatata tcaagtattg
     1501 ccaggacaat gctcggtgaa gttgtggaaa aaattgatga ttggcaaata gaacgtttcc
     1561 aacaatctat tcgagatgac aaaatatcat ttaccataca aacgaatcat tcagaaaaat
     1621 atagtatgct atcggacatg cgcgctcata ttctacgtag ggataataat tatcagttta
     1681 ttgtgacaat taatagtaaa aattacggct gctatcttga taataccgat attaattggt
     1741 gcagcattgt ttatttactt aataatatga cagtaaatga tgatgctaat gatgttgcag
     1801 ttactgagtc gtacaaaccc gtctgggact ggaaaatatc gcaattcaat gtatctgata
     1861 ttaaattcga aacaatgata aacccccagt tcgctgacag aacctatttc tccaattgtt
     1921 cacctgttga tcccacaagt accagaccaa cctattttgg cgataccgat ggctcagtcg
     1981 gtgcagtttt gtacgcgctt tttgcaacgg gacatttgag aatcatggct gaaggagaaa
     2041 atttccttag ccagttgctt aatatagaag atgaagttct caacgtattg ctaagggaga
     2101 actttaatga gcaacttgac actaatgtta atacaataat cagtattctg aacagaagag
     2161 atatagtttt agaaagtctt cagccttatt tagttattaa taaagacgct gtgacgccat
     2221 gtacgtttct tggcgaccaa accggcgatc gatttagtaa tatctgtggc gatcaattta
     2281 tcatcgattt gttaaaacgc ataatgagta tcaatgaaaa tgttcatgta ttagctggaa
     2341 atcatgaaac gaattgcaat ggtaactata tgcagaactt cacgcgtatg aaaccacttg
     2401 atgaggatac ctacgctggg ataaaagatt atcctgtatg tttctatgac cccaaataca
     2461 aaataatggc aaatcatcat gggataactt ttgatgagca gcgaaaacgt tacattatag
     2521 gaccgataac agtctctatt gatgaaatga ccaatgctct cgatccggtc gaattagcgg
     2581 caattatcaa taaaaagcat catgctataa ttaatggcaa aaagtttaaa actagccggg
     2641 ctatctcttg tcgatccttt aatcgttatt tttctgtttc gacagattac agacctaaac
     2701 ttgaagcact attagcatgt tcacaaatgt taggtataaa tcaagttgtt gcacataacg
     2761 gaaatggtgg aagagaacgc ataggcgaaa cggggacagt tttaggtctt aacgcccgtg
     2821 acagtaaaca tgctggaaga atgttcagca tgcataattg ccaaattaac cctggtgcag
     2881 ggcctgagat aaccactccc tggaagtctt accagcatga aaaaaacaga aatggactta
     2941 tgcctttgat tagaaggcgc acaatgttac agctctgaac catagacaac atgaatatta
     3001 atgttactat ctggttgaga ctgttgataa aacgtaaaaa ggaatgctct gttcctgcaa
     3061 ctgaacaggc gtgaaacgct atccaacagg atggataccg ttttgtcagc cagttttata
     3121 gatcacactt atgagtatgc gtgtttcatt tacggccagg aatgctgatg atgacaacat
     3181 tactacccgt tttcactaag ccttccccgt tggcgctcaa tgctctgcgc gctggtcgta
     3241 tttgccgttt ccttcttatc ccggatggaa gaatccgctg aacgtcgttc ggcggcagtt
     3301 gtaacacgcg tcgtttgctt acagggatta tggcgggtcg attatggata agagtaagcg
     3361 ccatctggcg tggtgggttg tcgggttact ggcggtggcg gctatcgtgg cgtggtggct
     3421 gttgcgcccg gcaggtgtgc cggaaggctt tgctgtcagc aatgggcgca ttgaagcgac
     3481 ggaagtggat atcgccagca aaattgccgg gcgtattgac accattctgg tgaaagaagg
     3541 ccagtttgtt cgagaaggtg aagtgctggc gaagatggat actcgcgtgt tgcaggaaca
     3601 gcgactggaa gccatcgcgc aaatcaaaga agcacaaagc gccgttgctg ccgcgcaggc
     3661 tttgctggag caacgccaaa gtgaaactcg cgccgctcag tcgctggtta atcaacggca
     3721 agctgaactg gactccgtag ccaaacgtca tacccgttcc cgctcgctcg cccatcgtgg
     3781 ggctatttct gcgcaacagt tggatgacga ccgcgccgcc gctgagagcg cccgagctgc
     3841 gctggaatcg gcaaaagctc aggtttcggc ttctaaagcc gctatagaag cggcacgcac
     3901 caatatcatt caggcgcaaa ctcgcgtcga agccgcacaa gccactgaac ggcgcattgc
     3961 cgcagatatc gatgacagcg aactgaaagc cccgcgtgac ggacgcgtgc agtatcgggt
     4021 tgccgagcta ggcgaagtgc tggcggcagg cggtcgggtg ctgaatatgg tcgatctcag
     4081 cgacgtctat atgactttct tcctgccaac cgaacaggcg ggcacgctga aactgggcgg
     4141 tgaagcccgg ctgatcctcg atgccgcgcc agatctgcgt attcctgcaa ccatcagttt
     4201 tgtcgccagt gtcgcccagt tcacgccaaa aaccgtcgaa accagcgatg aacggctgaa
     4261 actgatgttc cgcgtcaaag cgcgtatccc accggaatta ctccagcagc atctggaata
     4321 tgtcaaaacc ggtttaccgg gcgtagcgtg ggtgcgggtg aatgaagaac ttccgtggcc
     4381 tgacgacctc gtggtgaggt tgccgcaatg acgcatctgg aactggttcc cgtcccgcct
     4441 gtcgcgcaac tggcgggcgt gagccagcat tatggaaaaa ccgttgcgct gaacaatatc
     4501 acactcgata ttccggcccg ttgcatggtc gggctgattg gcccggacgg cgtcgggaag
     4561 tcgagcttgt tgtcgttgat ttccggtgcc cgcgtcattg aacagggcaa tgtgatggtg
     4621 ctgggcggcg atatgcgcga cccgaagcat cgccgcgacg tctgcccgcg catcgcctgg
     4681 atgccgcagg ggctgggcaa aaatctctac cacaccttat cggtgtatga aaatgtcgat
     4741 tttttcgctc gcttgttcgg tcacgataaa gcggagcggg aagtccgaat taatgagctg
     4801 ctgaccagca ccgggttagc accgtttcgc gatcgtccgg caggtaaact ctccggcggg
     4861 atgaagcaaa aactggggct gtgctgcgcg ttaatccacg acccggaact gttgatcctt
     4921 gatgagccaa caacgggggt tgacccgctc tcccgcgccc agttctggga tctgatcgac
     4981 agtattcgcc agcggcagag caatatgagc gtgctagtcg ccaccgccta tatggaagag
     5041 gccgaacgct tcgactggct ggtagcgatg aatgccggag aagtgctggc aactggcagc
     5101 gccgaagagc tgcggcagca aacgcaaagc gccacgctgg aagaggcgtt tataaatctg
     5161 ttaccgcaag cgcaacgcca ggcgcatcag gcggtagtga tcccaccgta tcaacctgaa
     5221 aatgcagaga ttgccatcga agcgcgcgat ctgaccatgc gttttggttc cttcgttgcc
     5281 gttgatcacg tcaatttccg cattccacgc ggggagattt ttggttttct tggatcgaac
     5341 ggctgcggta aatccaccac catgaaaatg ctcaccggac tgctgcccgc cagcgaaggt
     5401 gaggcgtggc tgtttgggca accggttgat ccaaaagata tcgatacccg ccgtcgggtg
     5461 ggctatatgt cgcaggcgtt ttcgctctat aacgaactca ccgtgcggca aaaccttgag
     5521 ttacatgccc gtttgtttca catcccggaa gcggaaattc ccgcgcgagt ggctgaaatg
     5581 agcgagcgtt ttaagctcaa cgacgttgaa gatgttctgc cggagtcatt gccgctcggc
     5641 attcgccagc ggctttcgct ggcggtggcg gtgattcatc gcccggagat gttaatcctc
     5701 gatgaaccta cttctggcgt cgatccggtg gcgagggata tgttctggca gttgatggtc
     5761 gatctctcgc gccaggacaa agtaaccatt tttatctcca cccactttat gaacgaagcg
     5821 gaacgttgcg accgcatctc actgatgcac gccggaaaag tgctcgccag cggtacaccg
     5881 caggaactgg ttgagaaacg cggagccgcc agtctggaag aggcatttat cgcctatttg
     5941 caggaagcgg caggacagag caacgaagcc gaagcgccgc ccgtgataca cgacaccacc
     6001 cacgcgccgc gtcagggatt tagcctgcgc cgtctgttta gctacagccg ccgcgaagca
     6061 ctggaactgc gacgcgatcc ggtacgttcg acgctggcgc tgatgggaac agtgatcctg
     6121 atgctgataa tgggttacgg catcagtatg gatgtggaaa acctgcgctt tgcggtgctc
     6181 gaccgcgacc agaccgtcag tagccaggcg tggacgctca atctctccgg ttcccgttac
     6241 tttatcgaac agccgccgct caccagttat gacgagcttg atcgtcggat gcgtgcgggc
     6301 gatataacgg tggcaattga gatccctccc aatttcgggc gcgatatcgt gcgtggtacg
     6361 cctgtggaac tcggcgtctg gatcgacgga gcgatgccga gccgtgccga aacggtaaaa
     6421 ggttacgtgc aggcaatgca ccagagctgg ctacaggatg tggcgagccg acaatccaca
     6481 cccgccagcc aaagcgggct gatgaatatt gagacgcgct atcgctataa cccggacgta
     6541 aaaagcctgc cagcgattgt tccggcggtg atcccgcttc tgctgatgat gatcccgtca
     6601 atgctaagcg cccttagcgt ggtgcgggaa aaagagcttg ggtcgattat caacctttac
     6661 gtgaccccca ccacgcgtag tgaatttttg cttggtaaac agttgccata catcgcgctg
     6721 gggatgctga actttttcct gctctgcggc ctgtcggtgt ttgtgtttgg cgtaccgcat
     6781 aaaggcagtt tcctgacgct caccctggcg gcgctgctgt atatcatcat tgccaccgga
     6841 atggggctgc tgatctccac ctttatgaaa agccagattg ccgccatttt cggaacggcg
     6901 attatcacgt tgatcccggc gacacagttt tccgggatga tcgatccggt agcttcgctg
     6961 gaagggcctg gacgttggat cggcgaggtt tacccgacca gtcattttct gactatcgcc
     7021 cgcgggacgt tctcgaaagc gctggatctg actgatttgt ggcaactttt tatcccgttg
     7081 ctgatagcca tcccgctggt gatgggctta agtatcctgc tgctgaaaaa acaggaggga
     7141 tgatgcgcca tttacgcaat atttttaatc tgggtatcaa agagttgcgc agtctgctcg
     7201 gtgataaagc gatgctgacg ctgattgtct tctcgtttac ggtgtcggtg tattcgtcag
     7261 cgaccgttac gccaggatcg ttgaacctcg cgccgatcgc cattgccgat atggatcaat
     7321 cgcagttatc gaaccggatc gttaacagct tctatcgtcc gtggtttttg ccaccggaga
     7381 tgatcaccgc cgatgagatg gatgccggac tggacgccgg acgctatacc ttcgcgataa
     7441 atattccgcc taattttcag cgtgatgtcc tcgccggacg ccagccggat attcaggtga
     7501 acgtcgatgc cacgcgcatg agccaggcat ttaccggcaa tgggtatatc cagaagatta
     7561 tcaacggtga agtgaacagc tttgtcgcgc gctaccgtga taacagcgaa ccgttggtat
     7621 cgctggaaac ccggatgcgc tttaacccga acctcgatcc cgcgtggttt ggcggggtga
     7681 tggcgatcat caacaacatt accatgctgg cgattgtatt gaccggatcg gcgctgatcc
     7741 gcgagcgtga acacggcacg gtggaacact tactggtgat gccgataacg ccgtttgaga
     7801 tcatgatggc gaagatctgg tcgatggggc tggtggtgct ggtggtatcg ggattatcgc
     7861 tggtgctgat ggtgaaaggt gtactgggcg taccgattga aggctcgatc ccgctgttta
     7921 tgctgggcgt ggcgctcagt ctgtttgcca ccacgtcaat cggcattttt atggggacga
     7981 tagcgcgttc aatgccgcaa ctggggctgc tggtgattct ggtgctgctg ccgctgcaaa
     8041 tgctttccgg tggatccacg ccgcgcgaaa gtatgccgca gatggtgcag gacattatgc
     8101 tgaccatgcc gacgacacac tttgttagcc tcgcgcaggc cattctctac cggggtgccg
     8161 gattcgaaat cgtctggccg cagttcctga cgctgatggc aattggcggc gcgtttttca
     8221 ccatcgcgct gctgcgattc aggaagacga ttgggacaat ggcgtaagga agggaaactt
     8281 gacttcatac tgtgatgtca taaagtgaag tcataaggga atgtcatgta aacacaggta
     8341 caggctttac gaaagaaaca aaaaaacaca ctggatcagc tatttaaatc acctttacct
     8401 caaggaatca aatggactga tatagaatct ctggttaaag cgttaggcgg agaaattaag
     8461 gaaggtaggg ggtcgcgctg taagttcata ctaaatatga gcgttgcgtg tttccatcgg
     8521 cctcatccgt cgccagatac cgataaaggc gctgtagaaa gcgtgcgtga ctggttacta
     8581 agtatagggg taaaaccatg atcaaactga agacgccaaa ctctatggag attgccgggc
     8641 aacctgctgt tatcacttac gtgccagaac tgaatgcatt tcgtggcaaa ttcctgggct
     8701 tgtcaggtta ttgtgatttt gtctctgaca gcattcaggg gctgcaaaaa gaaggggaac
     8761 tttccctgcg agagtatctt gaggattgta aagctgcggg tattgagccg tatgtgcgta
     8821 cagaaaagat taagaccttt acgttacgct atccagaatc gctgagtgaa cgccttaata
     8881 atgcggctgc gcagcaacaa gtctctgtta atacctatat tattgaaaca ctgaatgagc
     8941 gtttgaatca tttataaatt ttagcggcgt acattacgca gaatatctgc ctttacagga
     9001 tctatctatg caactgaact taattttata aattaagttc agttaatatc ttttctttcc
     9061 tcgtttcaga aataactcaa tcttccttcg gcaggcactg caaatgcccg tgccgcacgc
     9121 cgcgctgggc gataacgtca tcggcaaaat gctgcacgtc gcccatatca cctttcagca
     9181 cggcgatttc cagacagtcg tcgtgattga tatgcacatg cagcgtggcg acggagagat
     9241 cgtggtgatg gtgctgggtg gagacaatgc ggctggctaa gtcgcgtttt tcgtgttcat
     9301 acacatagga gagcaccgcg aaaccttgcg tgccgtgctg ctgagtggcc tcctgggcca
     9361 gggcgctacg cagaatatcg cggatagctt cggaacggtt gttataacca cgacgctggc
     9421 tcaggctgtc cagcgtctcc agtaaatcgt catcaagcgt gatggtgact cgttgcattt
     9481 gcgttaaacc ttttctgtgg tgcgacggcg cacggggaat gcgggtaata ccgcgttttg
     9541 tagcacacgt ccggcgtcag aggaaaaggt taatttatct cccaccacct gggtttcgac
     9601 gatttgtccg ttgtccatca ccatcacccg ctgacagaag cgttccacca ggcgtaagtc
     9661 gtgggtgatg aacaggcagg cgctgccaaa ctgttgttgt agctttttca gcaggcgaat
     9721 gacgcccgcc tgtaacacga gatcaaggtt agaaacggct tcatccagaa tcagtagttt
     9781 cggttcgacc gccagcgcgc gagccaggca gacgcgctgg agctggccgc cgcttaactg
     9841 cggtgggcgt ttgtcgagaa cgctgtcatc gagatcgacc gccttcagca tttcgctggc
     9901 gcgcgccagt tgttcggatt ttttcagtga tagcaggtgg cgcatcggtt cacgcaggat
     9961 ctcgcgcacg gttttgcgcg gattcacggc gctgatggag tcctgaaata ccatctgaat
    10021 atcgcggcgg aacgctttac gctgggcgcg attgagtttc gccagcggtt cgccgcgcca
    10081 gctaatattc ccttgtgagg gagactctaa gcctaccagc agccgcgcga gggtgctttt
    10141 tccgcagccg ctgcgtccca gcagggcgac ggtttcgccg cttttcaggg tcagggaaac
    10201 gttattcagc accgcctgat gttgatgttt tccgctaaat ccaccgtggg catagtgatg
    10261 ggaaaggccg cagacgttaa gtaaggtcat gatgccagct ccataccgta gagggcgaga
    10321 tgagcggaaa ccaggctgcg cgtcaccgta tgtttggggg cgttaaacag cgtttctaca
    10381 tcgccctgtt cgacaatttt accttgtgac atcaccgcca cgtcatccgc cagacgcgcc
    10441 acaacgccca tatcatgggt caccagcagc attcccggcg cttgtttttg cataatgctt
    10501 tccagcagat cgaggatgcg cgcctgtgct accacgtcga ggtcggtggt cggttcatcg
    10561 gcgatgataa acggtgattc acacagcacc gccatcgcaa tcatcatgcg ctgcaacatg
    10621 ccgccgctca tctcgaacgg gtacagcttc agcacgcgcg cggcgttttc cagccccacc
    10681 gcttctatgg cagcggtaag cgtagcgtca tcggcgggtt tccctaacgc caggcaggtt
    10741 tcccgcgcgt gggtgtgcat ggtgtgcagt ggattaaagg cgctgcgcgg gttctgcatg
    10801 atggtggcaa ttttgatgcc gcgcagggcg caaggcgaaa ccggtttgcc atcggctaaa
    10861 atttcccccg ccgtctggcg aacgccagcg ggcaaaatgc ccagcgtcgc ggcgcaggtt
    10921 aatgatttcc cgctgccgct accgccgact aacgccagca cgcgcccgcg ttgcagggtt
    10981 aacgatacac cgtgtaccag cggctgcgcg gcctgtagcg cgatattacg tagttcaatc
    11041 tgttgcggca ttagtgtgcg tgctccgtca ccagatgagg atccagatga tcgcgcagtg
    11101 cgtcacccac caggttaaag gccatcacgc tgataaacag cgccagcccc ggccagaaca
    11161 tttgcagcgg ctgggtccag atatactggc gcgcgtcgtt aatcatcacg ccccattcgg
    11221 cggtcggcgc ggtcacaccg aggccaagga aagacatccc cgcgacgtgc agcatcatat
    11281 ggccgatatc cagcgttgcc agcaccagca gcgaagggat caccgcgcct gccagatgat
    11341 cgacaaacac ccgcacatgg cccgcaccgg aaagccgtga cgccagcaca aactcgcgtt
    11401 ggcgtagtga aatcaccagg ctgcgcacca tgcgtgcata ccacgcccag tgcgacaggg
    11461 cgatggcgat aattacgttg gtcagcccgg tgccgagcac gccaaccata aagaacgaca
    11521 gaatcgaggt cgggaaggtc ataaacatat cggcgacgcg catggtggcc tgatcaacgc
    11581 gtccgccaat cagtccggcg ctgccgccaa tcaccagccc taatgtcagc accagcagca
    11641 ggcaggccat taccgaaccg agcgacacgc gggtcgccgc catcagccgc gagaaaatat
    11701 cacgccctaa gtgatcggtg cccagccagt gctgcgcatc cggcgaaagc aggcgcgacg
    11761 gcagatcaat agcctgaggg tcatacggca gccaccactg gctggtaagc gcaatcagcg
    11821 ccagcagggc gataatgatc agcgccaggc gtaccgacca gcgggaagag aggaaaaagt
    11881 tcacgcgtgc gctccttcat gacgacgaat gcgcgggtcc agcgcggcgt tgagcaaatc
    11941 gacaatcaaa ttacagacca caaaaaccac caccatcatc agcgtaaagc actggatcac
    12001 cggatagtca cggttaaaaa tcgccgacac cgcatagcgc ccgacgcccg gccaggcaaa
    12061 gatgttttcg ataatcatcg tcccgccgat cagttcgccg atgtgcatcc ccacggcggt
    12121 gatcatcggc agcgaggcat tgcgcaggat gtggcgacgt tcggtctgtt tgtcgttcag
    12181 gccgcgcaga cgcgcccagg tgacgtgacg ctgaccggcg acgtccagca tactggcgcg
    12241 cagtaaacgc gcgttaatcg ccagcgacat aaaggcaatg gaaaccgcag gcaaaatgat
    12301 gtgctgccag ccgccgtaac ccatcgcggg tagccattgc agatacaccg aaaacgccat
    12361 caccagtaaa aacgccagcc agaagttagg catcgacacg ccaagaaacg cgatgaaacg
    12421 tacggcgaaa tccggcagac ggtcgcggtg gcgcgccgcc cagataccga gcggtacgga
    12481 agtgagcaga attaatacca gcgccgcacc tgcaagttcc agcgtggcgg gcaggaagtt
    12541 cagcatatcg tccagtaccg ggcgttggct ggcgaatgag ataccaaagt caagatgcag
    12601 cgccttccac aaccaggtgc cgtactggac gtacagcggc tgatccagtc ccagcatggt
    12661 gcgggtagag gccagcatct ccggcgtcgg cggcaggtta gacagacgca aataatcgag
    12721 cgccgggtcg ccggtaccga ggcgcagcat cagaaaaatg atcaccgagg cggcgagcac
    12781 catcgggatc agcagcagaa agcggcgtaa tacgtaacgc aacattaagg tttcaccggt
    12841 ttaatctgtt cgaacggaat ttcggtggcg atcggcgcgt aggggatatt acccagctcc
    12901 ggttttgata ccaccatcat cgagatgtag ctgataggca gataaaccgc ctcgtcatgc
    12961 agacgggtca gtatgtcgcg atacagcgcc tgacgttgcg tttcgtcatg ggtcgccagc
    13021 acttcgccga tctctttatc aatcagcggc ttatcggcta atccttgctg tgcctggaag
    13081 tcagcgtgcg acggtacgcg cattgaactc aggaaggcgt gtggatcgta tggcgcgccc
    13141 caggtgcggt ggaaaatcat gccaaaacga ccgtcgcgct gacgagcata gatactgctc
    13201 tcttcttcgc caatcagcga gacatctgcg ccaatctggc gcatatcagc ctgaatgatt
    13261 tccgccatcg atttgcttaa cgcatcggtg ccgatgaacg acagttcaat gcgcagcggc
    13321 tgaccatttt tctcgcggat gtctttgccc gcaggcagcg tccaaccggc tttttccagc
    13381 aacgctttcg ctttctgcgg atcgtactgg cgcggtttca ggccgagatt agcgtaaggc
    13441 acagaagggg caaacagggt gtcggcgacc tgctgggtgc catataacgc gttatcaatc
    13501 agcgattttt tgtttaccgc gtaattaaga gcttcacgta ctgccagctc gttggtgggg
    13561 gctttggcgg tattgagcgc cagcatcacg gtttcgatcg gctgtgacag ttgggtgtgg
    13621 taagccggat tctggctaaa gcgggcgaag gtatcgagcg gtaataaccc ttcgtttccg
    13681 tacagcaggt cgatatcgcc agtttcaaac gccaccgcgc gggtagtcgg gtccgggatg
    13741 acgttaaagg tgatcttttt aatcgctggc ttttcgcccc agtagttttc gttacggacg
    13801 aagacatcgt actgattcag tttcgattcc tgcaaaatcc atggtccggt gccaatcggc
    13861 gctttaattc cgttcatggt ttcatggttt ttaaactgcg agggagcgat aaaacggaaa
    13921 ggacggggca gggccagttc ttgcaggaaa ggatagtagg cgcttttcag ggtaatttgc
    13981 agctctgttt tactgagtgc tttaacatca acaatctggt ttgccagctc cagccaggcg
    14041 tgacgttgac ggttatcgag cactgcgcgg aagttttctg ccgccgcctc ggcatcgaac
    14101 ggttcaccgt tggagaattt cacgtcatca cgcagggtga aggtccaggt tttaccatct
    14161 tctgaatgag tccagctttt tgccagccac gggatcaccg aaccgtctgc ctgatatttc
    14221 accaatggtt cataaaccat gctctgggcg aacatctggt taggcgtgta aaggtgcggg
    14281 tttagtggcc cgacattcac cggccaggcg gtagtgattt catctggtgc agcggcatgg
    14341 acgataaaag acgcacaagc cagcagcgca aatagagtgc ggcggagtgt ggagagcatg
    14401 ataaccccaa tggattaaaa tagatggcgg aaataagtat gacgatttta agtattcgtc
    14461 atactgatta cctgttctgg atcaatagat gggcagtcgg gtgggcggat cagttaactg
    14521 aatcgatcca ttgcacactg tcggcggtga gcgtaaaggg ggtgggggtg cagatcgcca
    14581 ggcttaaatt ttcaagctga caatggctga ctgacagcga ggagtgatag gtgctgtcta
    14641 cgctgacgat ttgccaggcg ctgccgccac gctgtttaac gatggcttct ttgcgcgtcc
    14701 agatgcgcca gaacatttcc agttgctgat caggatgcac ggcgtccatc tcagcgtgtt
    14761 ccccgaggct gaatacggcg ttcgccagcc agcgccagtt ggcgcgcggg cgaatcactt
    14821 cgatatcgca gccgacttcg ccttcatcac tcaacagcag agcgatatcg tcaccgctat
    14881 ggcttaagtt gaaccatagc ggcgtttccg gcgcgaatgc aggtttgcct tgttcgccat
    14941 agatgatctc cggtagcggg gaaagcgtgt gcgaaagcaa tgcacgcccc gccagccagc
    15001 gttcgcgtcg tggaccttgc ggtgcttgct cgcgtaaacc cggtggcagt ggagctgcgc
    15061 ttaaggtcga aactttcccc agaactatcc gatacatatc agggccaacg tttaatggaa
    15121 aatgaaagtg cgtatcgtat cacttgtcgc ctcatcccgg taaccgactt ttcggtctgc
    15181 ccggccccag taaaatcgcc agtttgctgc cacctttcgt cgtttccatc cagattttac
    15241 acacgctggt taatggcacc gaaagtagca tccctaccgg gccgagcagc catccccaga
    15301 ttaacaatga aagaaatacc accatggtgg acatccccag gcgatggccc atcatccgtg
    15361 gctctaaaat attgccgatg accatatgga caactaaaaa caatgcgccg accagaatac
    15421 attcgtaaat accattaaac agcagcacct gaatcattgg cggtacggcg gaaattaccg
    15481 cgccgatatt gggcacgtag ttaagcaaaa acgccagtac tgcccacatc agcgcaaact
    15541 gtacccccat cagctccagc cccagccaga cgatgacacc tgtccataaa ctgagtagcg
    15601 tcttcaatgc aagatagtgc gaaacgcctt taagtgcgcg gtgtaatccc gcgatgtgaa
    15661 tctgtggatt attcagcgca aaacgcattt tgtaagggac gtggcgcact tcaaacagca
    15721 taaaaactac ggtcatcacc agcaaaagca cgctcgccat tgccccggaa agcccggtca
    15781 ttagcgctgt ggtgaaggta accacttttt ccgagtccat ccgctgcagc attcgctccg
    15841 gcgacatatg caaattaaga aaaggcaaca tctcctgcaa tttaaaaagt ttgcgcgtca
    15901 gctccttatt aaacttcggc agcatagaga taaattcgtt aaacgatgcc gccagtacgc
    15961 cgaccagcgc ggttagtgcg atcagcatca ccaccactac aatcgtaatg gcaacggggc
    16021 gttgtactcc ccgacgaata aaccaggtga cgagcgggtt gaggacgatg gcaaaaaaca
    16081 gcgccagtaa cagctgcaca atgatatctg ccgctgcgtg aatgcccgcg aggatcacta
    16141 ccagcgaggc cagcttgagc agaatgtgca tgcccgtttt atcgggttga ggggtttcca
    16201 ttggggcttc cttgtgactt tttgtattaa gtgtagcggg agtcacgcca gcaatattct
    16261 gattcttact gctgattttc acgtcagcac atggtaaaaa tgaaacactg ttgtaaaaat
    16321 gtggtgatcc tcatgcccga acccgtagcc gaacccgcgc taaacggatt gcgcctgaat
    16381 ttgcgcattg tctccattgt catgtttaac ttcgccagct acctcaccat cgggttgccg
    16441 ctcgctgtat taccgggcta tgtccatgat gtgatgggct ttagcgcttt ctgggcagga
    16501 ttggttatca gcctgcaata tttcgccacc ttgctgagcc gccctcatgc cggacgttat
    16561 gccgatttgc tgggacccaa aaagattgtc gtcttcggtt tatgcggctg ctttttgagc
    16621 ggtctggggt atctgacggc aggattaacc gccagtctgc ctgtcatcag cctgttatta
    16681 ctttgcctgg ggcgcgtcat ccttgggatt gggcaaagtt ttgccggaac gggatcgacc
    16741 ctgtggggcg ttggcgtggt tggctcgctg catatcgggc gggtgatttc gtggaacggc
    16801 attgtcactt acggggcgat ggcgatgggt gcgccgttag gcgtcgtgtt ttatcactgg
    16861 ggcggcttgc aggcgttagc gttaatcatt atgggcgtgg cgctggtggc cattttgttg
    16921 gcgatcccgc gtccgacggt aaaagccagt aaaggcaaac cgctgccgtt tcgcgcggtg
    16981 cttgggagcg tctggctgta cggtatggcg ctggcactgg cttccgccgg atttggcgtc
    17041 atcgccacct ttatcacgct gttttatgac gctaaaggtt gggacggtgc ggctttcgcg
    17101 ctgacgctgt ttagctgtgc gtttgtcggt acgcgtttgt tattccctaa cggcattaac
    17161 cgtatcggtg gcttaaacgt agcgatgatt tgctttagcg ttgagataat cggcctgcta
    17221 ctggttggcg tggcgactat gccgtggatg gcgaaaatcg gcgtcttact ggcgggggcc
    17281 gggttttcgc tggtgttccc ggcattgggt gtagtggcgg taaaagcggt tccgcagcaa
    17341 aatcaggggg cggcgctggc aacttacacc gtatttatgg atttatcgct tggcgtgact
    17401 ggaccactgg ctgggctggt gatgagctgg gcgggcgtac cggtgattta tctggcggcg
    17461 gcgggactgg tcgcaatcgc gttattactg acgtggcgat taaaaaaacg gcctccggaa
    17521 cacgtccctg aggccgcctc atcatcttaa aacttactga ataaccagcg tattaatgat
    17581 gttttctgcg gtggtctgcg ctttttgctg atcgtcagcg ggcagcgtaa tttgcatggt
    17641 cagcagttga ttacccacgt tacccagaat aacggaagag tacgccgtct ggcctttcgc
    17701 ggagataata ctgtctaact gctgcatttt gtgacctttc agctcaatgg ctttattggt
    17761 taccacttgc agctgcggat cgcggctacg ttgctgatct tccagacgct tcgccagcac
    17821 cgccagatct tctttcggat catcgcccat gatgacgatg actgctttct gcccggtggc
    17881 gtcggaccag acatgcatgt tattggcctg cgttcccagc ttaccgctct ggtcggtcat
    17941 atccgctggc agcgagaaac ttaacttgcc atcaagcagg ttgacgggat tcccggtagc
    18001 gttactttcc gcgaccgaac cctgcgccgt agcgttagtg tctttatcat cacaggccgc
    18061 aagccccata accagcaggc caattccgac atatttaacc agattgcgca ttgacttctt
    18121 cctttcgata aacggccata acggctcatt catccatctt atcacaactc tgataacgaa
    18181 cctttaactc gcctgcaaag cgttgatttc ggatttgtct gccagtcttt tcaacagcat
    18241 attgagtaat acgccataca ttggcaggaa gaaaacgata ctgattaaca ctttgaaaca
    18301 gtaatcgacc agcgcgattt ccatccagtg ttcagccata aaggcatccg ggctacgcca
    18361 gaaggcaatg aagaaaaagg ccagcgtgtc actgacgtta ccgaacagtg tggacgctgt
    18421 cggtgccagc caccagcggc gactttgacg caggcggtta aaaacgtgca cgtcgaggat
    18481 ttgccccagc gcgtaggcca tgaaactggc ggtggcgata cgggcgacaa acaggttgaa
    18541 gtgggcgagt gcgccgaatc cctgccatga acccatatag aacagcgacg agatgacata
    18601 ggagattaat aacgcaggga tcattaccgc gaagataatg cgtcgggcca gcggtgcgcc
    18661 aaaaatacgc acggtcaggt cggtcgcgag aaagataaac ggaaaactaa acgcgcccca
    18721 ggtggtatga aaaccaaaaa tggagaccgg aagctggacc agatagttac tggaggtgat
    18781 caccagcaga tgaaataacg ataaccagaa caacgcctta tagcgttgag tttgcgagaa
    18841 aacgttcata ttgtaccttt ttgattaacc attggggtga gggaacccaa tacgtacgac
    18901 acgtctgtta tcttaacata ataacgtgct tacccttttt tcgagccgcc gcatgatact
    18961 gctttacgtt gacaatgcaa tggttaattt tcacgcaatc gttaacctgg tttgcttacg
    19021 gactcacagg gcgtaaacta gcgccgtttt tttaagtgat gagaagaaaa tgaccgatct
    19081 cttttccagc cctgaccaca cactcgacgc gcttggcctg cgctgcccgg aaccggtgat
    19141 gatggtgcgc aaaaccgtgc gcaatatgca gcctggcgaa actttactga ttatcgccga
    19201 cgatccggcg acgacccgcg atattcctgg gttttgtacc tttatggaac acgaactggt
    19261 tgcgaaagag acggatggac tgccttatcg ttatttgatt cgtaaaggcg gttgatgggg
    19321 aaagattggc ttcgatgccg ccttttcccc tcaccctaac cctctcccca gaggagcgag
    19381 gggaccgatc gcgctcaatg ttgcgatcgg tttgccttat ctcctgcgca acaatcttaa
    19441 cgcattcgct gttaccagca cggtcgcacc ggtatctgcc agcaccgcca gccatagtcc
    19501 ggtcatccct agcagcgtgg tgacgaggaa gatccctttc agccccagcg caatagtgat
    19561 gttctggcgg atattggcgt gagtggcgcg tgccagttca atcatttgca ccaggccgcg
    19621 caggtggtta tgggttaatg ctgcgtcggc ggtttccagc gccacgtctg tgccactgcc
    19681 cattgcaatc ccaatggcgg cagctttcat cgctggcgcg tcgttaatac catcaccgac
    19741 catcgccagg ggcgcatgtt gattcagctt ggtcaccgct ttgactttat cttccggcaa
    19801 taggcccgct ttaaactcca gccccagctc cccggcaatt gccgccgctg cgcgtggatt
    19861 atcgccggtg aggatcactc ctttaacccc cagcgcgttc agttcactga tggcagttgc
    19921 tgcgtcggcg cgcagggtat cctgcaatgc aatgataccc agcacgtcat cattacgtac
    19981 taccagcaca accgtttgcc cggcgctttc cagttcatta atcaaaccag caaatgcatc
    20041 agcgggatgt ttcccggcag cgcaaatcaa tacgcgctca ccgttaacct gcgcttcaat
    20101 gccagacccg accagcgccc gctgtgattc ggcggtggga atggcgagtt cagcaacctg
    20161 tgcttcgcgt acgatggcct gcgccagtgg atgcgtcgcg ccttgctcga ccgccgccgc
    20221 cagtgtcagc agttcagatt cactaatacc cgttgccgga tgaatcgcgg taacgcgcgg
    20281 tttaccgacg gtcagcgtac cggttttatc aaacgccacc tgagtaacac gacccagctg
    20341 ttccagcgcc gctccgcctt taatcaacgc cccacgacgc gctgccgccg ccagcccgga
    20401 ggtaatcgcc gcaggcgtgg agataactaa cgcacacggg cagccaatca gcagcagcgt
    20461 cagcccttta taaatccact cctgccagct ggcggcaaac agcagcggtg gcaccagcgt
    20521 caccagcagg gcgacggcca taatcgctgg cgtataaata cggctgaaac ggtcgataaa
    20581 ccgctcaatg ggggcgcggc gctcttcggc ttcttcgatc agtttcagaa tccggtcaat
    20641 ggcgctggca cccggttctg acagcacttc cagcgttacc agacggtcta cgctggtggc
    20701 tcctgcagga actttatcgc ccgtcgcgcg ctccaccgga atggattctc cggtcagggc
    20761 gctttcatca aaactggcaa acggtgagag cagtttaccg tcggcaggca aacgcccacc
    20821 tgcggcgact tcaatcacat cgccaggccg caggctgtta atcgccacct cttcccgctc
    20881 accgttacgc aggcgcgtgg cggtttctgg tttcagcgcc attaacgcgc taacgccctg
    20941 acgcgcgcgg ctggcggccc agccttccag tcgttcacca atcaaaaaca gcagcaacac
    21001 catcgcagct tcagccgttg cgccaataaa cagtgcacca atagcggcta cgctcattaa
    21061 ggtttcaatg gcgaagtagc tgccggattt gatcagccgt aatgcctgac gagcaatcgg
    21121 gtacagccca accagcgtgg tcgcgataaa cgccagttgc ccaaacggat gattaaattg
    21181 ctccagaccc cagctgattg ccatcatcac gattagcgta atcagcggca gattctcttt
    21241 caggcgcgat gcttgtggct cgtcggtggc ctgctcatcg cgcagggaat agcccgcttt
    21301 ttgcaccgca gattcaacct gggcacggat gtcattgtcg gcatcgacca ccagtttttc
    21361 ggtggcgaac agtacctgca cctgattcac acctgcaagc tggcgcacgg cattttctac
    21421 cttgcgcgca caggcggcgc agtccatgcc gctgactttc cagctatagc gggtgccgga
    21481 gacgttttca gagagcgttg gcgagctgga gcatgcgccg tcgcagcaac agtcgttggc
    21541 gttctgtacc gtggttagcg gtttgaacgc agcaaattga ggggctttct tgccgtgatt
    21601 gtcaggtgtc gacatggcat cctccggtta agttttttct cattaaccga aggatacact
    21661 ctggagtcga ctccagagtc aagttttatc agagatacag tgagcggacg atgaggaagt
    21721 gaccggcaaa gtagcaggcc gcagcaatcg cgttatccgc acggaagcgt cggcgatagt
    21781 ggctccccag ccagacaaag ttactgataa acagcaacga agcgccaaca aacgcagaga
    21841 gcgccggagc ggtcggacgg aagaaccaca gttcacctgc cagccacacc atcaccagcg
    21901 tcatgccgat aaacgtgcag ataggccaac gatactcttc cagccgcgtc cagataatcg
    21961 ccagtaacag cgcacccaga accagcaaca ccagtggcag aggccagaag aaagagagcg
    22021 tcatctgact ggcgaaatag atggtgtaca gcaggtgcga gaggaaaaac gcgccgatgg
    22081 cgtacatcag acgttgacgt ggcaacaggg ttagcgcatc gcccagcagt gaggcgcaca
    22141 gccctgccag caccagatag ctaatggcgt cgaacatcgg cgcttgccag gccagtaaca
    22201 gcaggagaag aagggttaac ggtttaaaca cccagcgttg ccaggttggc ccacgatacg
    22261 atgcatccac agatagccat gcggaaagac agacagcgat aaacgaccaa agcatcttag
    22321 ttccttgaat tcgttatttt ttcgtaagcg gaatgcctac ggtcttgctt ccagtttagt
    22381 ggcgagggtg gacggatgac aacaccataa ctggcagggt atgcttattt ccgcattttc
    22441 cgatgaggga taaagatgag caaaccacct cttttcttta ttgttatcat ttgcttaatt
    22501 gtcgtcgccg catcgtttcg ttttatgcaa cagcgacggg aaaaagctga taatgatatg
    22561 gctccgctcc agcaaaagct ggtggtggtg agcaacaagc gggaaaaacc gattaacgat
    22621 cgccgttcgc gccagcagga agtgactccg gcaggcacca gtatgcgcta tgaggcaagc
    22681 ttcaaaccgc aaagcggagg aatggagcag acgtttagcc tcgacgccca gcagtaccac
    22741 gccctgacag tgggcgataa aggtacgctg agctataaag gaacgcgctt tgtcagcttt
    22801 gtaggcgaac aataaggtta ttttttaact ttaaatttct tctgccagac cagcagttca
    22861 aacacgccga aaaggaaaat ccgcaccttt tcggcggtgg tcatctgcgg gccatctttc
    22921 ggtaaagtgg atttcaatag cgccagttgc ataccatgca tcagcacggt aaaaatcagc
    22981 gccacgttaa cgaaaatatt cagcgggcgc gggaagggat gcaccagatt gaggattaaa
    23041 aatccccaaa cgcagagcat taacaaacga ccaatattaa tcagcatcac tttctccttg
    23101 tgcttcgcgt tgatacagcc gataagccac ctgacccgcc actttttccc gatgtaatga
    23161 ccagtttgcc ggaacagtgg gcagaccgtt ttcgacttcg ctttcgacat aaatcagggc
    23221 ttcgtcagcc agccagccgt tatcttccag taaatttatc gtctcttcta acaagccacg
    23281 gcggaacggt ggatcgacaa acacgatatt atgcggtgta cctttttgcg ccaggaatga
    23341 cattgcgttg ctgttcacca cgcgtgcatt gcctgctttt agtgtcgcca gattcttaat
    23401 taactgctga gaaaccgcgc gatccatctc aatcaacgtt gcccccgcag catagcgcga
    23461 caacgcttcc agccccagcg cgccgcttcc ggcgaagcaa tccagacatt gggcgtcaac
    23521 aatgaccgga gccagccagt taaacaacgt ttcgcgtacg cggtcggtgg tggggcgcag
    23581 acctgggcta tcaggaaccg ggagtttacg gcctcgccac tgcccgccaa taatgcggat
    23641 ttggccgctg ccggaatgat tcggtttttt catgataaat tgctcaatcc gccagataac
    23701 acatacttgc aggcggtgat gtgaattaag ttaagtgata gactatttca tcattttttt
    23761 agctgctatg tacatagcgt taacgctgtg ccatgaagca acagcgagga gtgtagtcgc
    23821 aaatggcgaa agaaaaaaaa cgtggctttt tttcctggct gggctttggt caaaaagagc
    23881 agaccccgga aaaagagaca gaagttcaga atgaacaacc ggttgtagaa gaaatcgttc
    23941 aggcgcaaga gcctgtgaag gcttcggaac aagccgttga agagcagccg caggcgcata
    24001 ctgaagccga ggcggaaact tttgctgccg acgttgtgga agtcactgaa caggttgctg
    24061 aaagtgaaaa agcgcagcct gaagcggaag tcgttgcaca gccggaaccg gtcgcagaag
    24121 aaacgccgga gccagtggct atcgaacgtg aagagctgcc gttgccagaa gacgtcaacg
    24181 ccgaagaggt ttcgccagaa gagtggcagg ctgaagcgga aaccgtagag attgtcgaag
    24241 cggcagaaga agaagcggct aaagaagaaa tcaccgacga agagctggaa gcacaggcgc
    24301 tggctgccga agcggcagaa gaggcggtga tggtagttcc tccggcagaa gaagagcagc
    24361 cggttgaaga aattgcccag gagcaagaaa aaccgaccaa agaaggtttc ttcgcgcgcc
    24421 tgaaacgcag cctgttaaaa accaaagaaa atctcggttc cggatttatc agcctgttcc
    24481 gcggtaaaaa aatcgacgat gatctgtttg aggagctaga agaacagttg ttgatcgccg
    24541 atgtgggcgt ggaaaccaca cgtaaaatta tcaccaatct gacggaaggc gcatcccgca
    24601 agcagcttcg tgatgccgag gcgctctatg gcctgctgaa agaagagatg ggcgagattc
    24661 tggcgaaagt cgatgagccg ctgaatgttg aaggtaaaac gccgttcgtg atcctgatgg
    24721 tgggcgtcaa cggtgtgggt aaaactacga cgattggtaa gctggcacgt cagtttgagc
    24781 agcagggtaa atcggtgatg ctggcggcgg gcgatacttt ccgtgcagca gcggttgaac
    24841 agcttcaggt ctggggtcag cgcaacaata ttccggtgat tgcccagcat accggtgcgg
    24901 attccgcctc tgttatcttc gacgccattc aggcagctaa agcgcgtaat atcgacgtcc
    24961 tgattgccga taccgccgga cgcctgcaga acaaatcgca cctgatggaa gagttgaaga
    25021 aaatcgtccg cgtgatgaag aaactcgacg ttgaagcgcc gcatgaagtt atgctgacta
    25081 tcgatgccag taccgggcag aacgcggtaa gccaggccaa actgttccat gaagctgttg
    25141 gcttaaccgg catcacgcta acgaaactgg acggcacggc gaaaggcggg gtaattttct
    25201 cggtggctga ccagtttggt atccctatcc gctacattgg tgtcggcgaa cgtattgagg
    25261 atttgcgtcc gtttaaggcg gacgacttta tagaggcact ttttgcccga gaggattaac
    25321 aatgattcgc tttgaacatg tcagcaaggc ttatctcggt gggagacagg cgctgcaggg
    25381 cgttacgttc catatgcagc cgggtgagat ggcgtttctg accggtcatt ccggcgcagg
    25441 gaaaagtacc ctcctgaagc tgatctgtgg gattgagcgg cccagcgccg ggaaaatctg
    25501 gtttagcggc catgacatca cgcgtctgaa aaaccgtgaa gttccgtttc tgcgccgcca
    25561 gattggcatg attttccagg atcaccatct actgatggac cgtactgtct acgataacgt
    25621 ggcgatcccg ctgattatcg ctggtgccag cggtgacgat attcgtcgcc gggtgtcggc
    25681 ggcgctggat aaagtcgggc tactggacaa agcgaagaac ttccctattc agctttccgg
    25741 cggtgaacaa cagcgtgttg gcattgcccg cgcggtggtg aacaagcccg cggtactgct
    25801 ggcggacgaa ccgactggta acctggacga cgcgttgtcg gaagggattc tacgtctgtt
    25861 tgaagagttt aaccgcgttg gggtaaccgt attgatggca acgcacgaca tcaacctgat
    25921 ttcgcggcgt tcctatcgca tgctcaccct gagcgatggt cacttgcatg gaggcgtggg
    25981 ccatgaataa gcgcgatgca atcaatcata ttcggcagtt tggcgggcgt ctggatcgct
    26041 tccgtaaatc ggtcggcggc tcaggcgacg gcggtcgtaa cgcaccgaaa cgcgcgaaat
    26101 cctcgccaaa accggtaaat cgcaaaacca acgttttcaa cgaacaggtg cgctatgcct
    26161 tccacggcgc attgcaggat ctgaaaagca aaccgttcgc cacgttttta acggtgatgg
    26221 ttatcgccat ctccctgacg ctgcccagcg tctgttatat ggtgtacaaa aacgttaatc
    26281 aggcggcgac gcagtattat ccgtcaccgc aaatcactgt ttacttgcaa aaaacgctgg
    26341 acgatgacgc cgctgcgggc gtggtggcac agttgcaggc cgagcaaggc gtggagaaag
    26401 tgaactatct ttctcgtgaa gacgcactgg gcgagttccg taactggtct ggttttggtg
    26461 gtgcgctgga tatgctggaa gaaaacccgc ttccggcagt ggcggtggtg atcccgaaac
    26521 tcgattttca ggggacggaa tcactcaata cgctgcgtga tcgtatcacc cagattaacg
    26581 gcattgacga agtgcggatg gatgacagct ggtttgcccg tctggcggcg ttgaccgggc
    26641 tggtcgggcg cgtttccgcg atgatcggcg tgttgatggt ggcagcagtg ttcctcgtca
    26701 tcggcaacag tgtgcgtctg agcatctttg ctcgccgtga ctccattaac gtgcagaaac
    26761 tgattggtgc gacggatggt ttcatcctgc gcccgttcct gtacggaggc gcattgctgg
    26821 gattttctgg cgcattgctg tcattaattt tgtcagaaat tctggtactg cgtttgtcat
    26881 cggcggttgc agaagtggca caggttttcg gaacgaagtt tgatatcaat ggtttatcgt
    26941 tcgatgaatg cctgctattg ctgctggtat gctcgatgat tggctgggtg gcagcgtggc
    27001 ttgccacggt acaacattta cgccacttta cgcctgaata ataaaagcgt gttatactct
    27061 ttccctgcaa tgggttccgt agcagggaaa gagatcccgt tgtctcttcc cggtatttca
    27121 tctctatgtc acattttgtg cgtaatttat tcacaagctt gcattgaact tgtggataaa
    27181 atcacggtct gataaaacag tgaatgataa cctcgttgct cttaagctct ggcacagttg
    27241 ttgctaccac tgaagcgcca gaagatatcg attgagagga tttgaatgac tgacaaaatg
    27301 caaagtttag ctttagcccc agttggcaac ctggattcct acatccgggc agctaacgcg
    27361 tggccgatgt tgtcggctga cgaggagcgg gcgctggctg aaaagctgca ttaccatggc
    27421 gatctggaag cagctaaaac gctgatcctg tctcacctgc ggtttgttgt tcatattgct
    27481 cgtaattatg cgggctatgg cctgccacag gcggatttga ttcaggaagg taacatcggc
    27541 ctgatgaaag cagtgcgccg tttcaacccg gaagtgggtg tgcgcctggt ctccttcgcc
    27601 gttcactgga tcaaagcaga gatccacgaa tacgttctgc gtaactggcg tatcgtcaaa
    27661 gttgcgacca ccaaagcgca gcgcaaactg ttcttcaacc tgcgtaaaac taagcagcgt
    27721 ctgggctggt ttaaccagga tgaagtcgaa atggtggccc gtgaactggg cgtaaccagc
    27781 aaagacgttc gtgagatgga atcacgtatg gcggcacagg acatgacctt tgacctgtct
    27841 tccgacgacg attccgacag ccagccgatg gcaccggtgc tctatctgca ggataaatca
    27901 tctaactttg ccgacggcat cgaagatgat aactgggaag agcaggcggc aaaccgtctg
    27961 accgacgcga tgcaaggtct ggacgaacgc agccaggaca tcatccgcgc gcgctggctg
    28021 gacgaagaca acaagtccac gttgcaggaa ctggctgacc gttacggtgt ttccgctgaa
    28081 cgtgtgcgcc aactggaaaa gaacgcgatg aaaaaacttc gcgccgctat tgaagcgtaa
    28141 tttccgctat taagcagaga accctggatg agagtccggg gtttttgttt tttgggcctc
    28201 tacaataatc aattccccct ccggcaaaac gtcaatcccc acgcagattg ttaataaact
    28261 gtcaaaatag ctattccaat atcataaaaa tggggtatgt tttagcagag tatgctgcta
    28321 aagcacgggt agtcatgcat aaaacgaaat aaagtgctga atcacaacac cacaacacac
    28381 gtaataacca gaagaatggg gattctcagg atgaacataa agggtaaagc gttactggca
    28441 ggatgtatcg cgctggcatt cagcaatatg gctctggcag aagatattaa agtcgcggtc
    28501 gtgggcgcaa tgtccggtcc ggttgcgcag tacggtgacc aggagtttac cggcgcagag
    28561 caggcggttg cggatatcaa cgctaaaggc ggcattaaag gcaacaaact gcaaatcgta
    28621 aaatatgacg atgcctgtga cccgaaacag gcggttgcgg tggcgaacaa agtcgttaac
    28681 gacggcatta aatatgtgat tggtcacctc tgttcttcat caacgcagcc tgcgtctgac
    28741 atctacgaag acgaaggcat tttaatgatc accccagcgg caaccgcgcc ggagctgacc
    28801 gcccgtggct atcagctgat cctgcgcacc accggcctgg actccgacca ggggccgacg
    28861 gcggcgaaat atattcttga gaaagtgaaa ccgcagcgta ttgctatcgt tcacgacaaa
    28921 cagcaatacg gcgaaggtct ggcgcgagcg gtgcaggacg gcctgaagaa aggcaatgct
    28981 aacgtggtgt tctttgatgg tatcaccgcc ggggaaaaag atttctcaac gctggtggcc
    29041 cgtctgaaaa aagagaatat cgacttcgtt tactacggcg gttatcaccc ggaaatgggg
    29101 caaatcctgc gtcaggcacg cgcggcaggg ctgaaaactc agtttatggg gccggaaggt
    29161 gtggctaacg tttcgctgtc taacattgcg ggcgaatcag cggaagggct gctggtgacc
    29221 aagccgaaga actacgatca ggttccggcg aacaaaccca ttgttgacgc catcaaagcg
    29281 aaaaaacagg acccaagcgg cgcattcgtt tggaccacct acgccgcgct gcaatctttg
    29341 caggcgggcc tgaatcagtc tgacgatccg gctgagatcg ccaaatacct gaaagcgaac
    29401 tccgtggata ccgtgatggg tccgctgacg tgggatgaga aaggtgatct gaaaggcttt
    29461 gagttcggcg tatttgactg gcacgccaac ggcacggcga ccgatgcgaa gtaatcatta
    29521 atcggcaact ttgggttgcc gccaaattgc taatatcgag tacgttgctt catgccggat
    29581 gcggcgtaaa cgccttatcc ggcctacaag atccaaagaa atcagtaaat tgcaacacac
    29641 attgtaggcc tgataagcgt agcgcatcag gcaatttctc gttggtcatc acttttgaaa
    29701 tcaacttgac gattaacgct tctcccagcc gccctgttgc gccgtaaacc ccagcgcctg
    29761 cataaacgcc gtcatcacac cgcgatcttc cacgcctgca tccgccatcc accagcatga
    29821 aacggcagga ttgttacgca aaacctcttc cagcagatat tgccccacac cgcgacggcg
    29881 ggtgacttcc cgcacgcgca gggaatccag tgctccctcg gtgccgctta aggttacccg
    29941 cacggccgct agcaggcgct cgttaaaacg cgcggcgtag atgcggtggt tatcgtcaac
    30001 ctgtaaagag gaaggggaat actccggcca gatcttttgc aggtcaatcc ggtcctggtc
    30061 gctaaagttt tctaatcgaa tgatggtcag cttcatgggg aacccgtgta aatcacaaaa
    30121 gtggaaccag tgtagcgaaa taatttaatc ggaggctttc tcttttttat ttcttttggc
    30181 aggtgattaa ttttttaaca gcaataatta caaagttaaa acattagaga atgaaaaata
    30241 cccagcataa tcccctgaat ggtagtgaat tattccgctc tttgtaccgt tattttatgc
    30301 tgacgacggc gcttttttct gtttatctat caataaattc agaatattat ctgttcttaa
    30361 tcgactgaaa aatagggatt ttaatcgcta ttatcacaaa atactgcgct aaacccttaa
    30421 tcagacaggc aaaaacagtg cagtataaaa aaagaacagt ctgatttgtt aacacataaa
    30481 aacaaagcaa cacaacatca cgaatgggga tttttgacta tgaaacggaa tgcgaaaact
    30541 atcatcgcag ggatgattgc actggcaatt tcacacaccg ctatggctga cgatattaaa
    30601 gttgccgttg tcggcgcgat gtccggcccg attgcccagt ggggcgatat ggaatttaac
    30661 ggcgcgcgtc aggcgattaa agacattaat gccaaagggg gaattaaagg cgataagctg
    30721 gtgggcgtgg aatatgacga cgcctgcgac ccgaaacaag ccgttgcggt cgccaacaaa
    30781 atcgttaacg acggcattaa atacgttatt ggtcatctgt gttcttcttc tacccaacct
    30841 gcgtcagata tctacgaaga cgaaggtatt ctgatgatct cgccgggagc gaccaacccg
    30901 gagctgaccc aacgcggtta tcaacacatt atgcgtactg ccgggctgga ctcttcccag
    30961 gggcctacgg cggcaaaata cattcttgag acggtgaagc cccagcgcat cgccatcatt
    31021 cacgacaaac aacagtatgg cgaagggctg gcgcgttcgg tgcaggacgg gctgaaagcg
    31081 gctaacgcca acgtcgtctt cttcgacggt attaccgccg gggagaaaga tttctccgcg
    31141 ctgatcgccc gcctgaaaaa agaaaacatc gacttcgttt actacggcgg ttactacccg
    31201 gaaatggggc agatgctgcg ccaggcccgt tccgttggcc tgaaaactca gtttatgggg
    31261 ccggaaggtg tgggtaacgc atcattgtcg aatattgctg gcgatgctgc cgaaggcatg
    31321 ttggtcacta tgccaaaacg ctatgaccag gatccggcaa accagggcat cgttgatgcg
    31381 ctgaaagcag acaagaaaga tccgtccggg ccttatgtct ggatcaccta cgcggcggtg
    31441 caatctctgg cgactgccct tgagcgtact ggcagcgatg agccgctggc gctggtgaaa
    31501 gatttaaaag ctaacggtgc aaacaccgtg attgggccgc tgaactggga tgaaaaaggc
    31561 gatcttaagg gatttgattt tggtgtcttc cagtggcacg ccgatggttc atccacggca
    31621 gccaagtgaa aatcccaccg cccgtgaaaa acgggcgggt ttagaaaggt taccttatgt
    31681 ctgagcagtt tttatatttc ttgcagcaga tgtttaacgg cgtcacgctg ggcagtacct
    31741 acgcgctgat tgccatcggc tacaccatgg tttacggcat tatcggcatg atcaacttcg
    31801 cccacggcga ggtttatatg atcggcagct atgtctcatt tatgatcatc gccgcgctga
    31861 tgatgatggg cattgatacc ggctggctgc tggtggcggc gggattcgtc ggcgcaatcg
    31921 tcatcgccag cgcctacggc tggagtatcg aacgggtagc ctatcgcccg gtgcgtaact
    31981 ctaagcgcct gattgcgctc atctcggcaa tcggcatgtc catcttcctg caaaactacg
    32041 ttagcctgac tgaaggttcg cgtgacgtgg cgctgcccag tctgtttaac ggtcagtggg
    32101 tggtggggca tagcgaaaac ttctctgcct ctattaccac catgcaggcg gtgatctgga
    32161 ttgtcacctt ccttgccatg ctggcgctga cggttttcat tcgctattcc cgtatggggc
    32221 gcgcgtgtcg tgcctgcgcg gaagacctga aaatggcgag cctgcttggc attaacaccg
    32281 accgggtgat tgcgctgacc tttgtgattg gcgcggcgat ggcggcggtg gcaggtgtgc
    32341 tgctcggtca gttctacggc gtaattaacc cctacatcgg ctttatggcc gggatgaaag
    32401 cctttaccgc tgcggtgctc ggtgggattg gcagcattcc gggagcgatg attggcgggc
    32461 tgattctggg gattgcggag gcgctctctt ctgcctatct gagtacggaa tataaagatg
    32521 tggtctcatt cgccctgctg attctggtgc tgctggtgat gccgaccggt attctgggtc
    32581 gcccggaggt agagaaagta tgaaaccgat gcatattgca atggcgctgc tctctgccgc
    32641 gatgtttttt gtcctggcgg gcgtctttat gggcgtgcaa ctggagctgg atggcaccaa
    32701 actggtggtc gacacggctt cagacatccg ttggcagtgg gtgtttatcg gcacggcggt
    32761 ggtctttttc ttccagcttt tgcgaccggc tttccagaaa gggctaaaaa gcgtttccgg
    32821 accgaagttt attctgcccg cgattgatgg ctccacggtg aagcagaaac tattcctcgt
    32881 ggcgctgctg gtgcttgcgg tggcgtggcc gtttatggtg tcgcgtggga cggtggatat
    32941 cgccaccctg accatgatct acattatcct cggcctcggg ctgaacgtgg ttgttggtct
    33001 ttctggtctg ctggtgctgg ggtacggcgg cttttacgcc atcggcgctt acacttttgc
    33061 gctgctcaat cactattacg gcctgggctt ctggacctgt ctgccgattg ccgggttaat
    33121 ggcagcggcg gccggcttcc tgctcggctt cccggtgctt cgcttgcgcg gcgactatct
    33181 ggcgatcgtt accctcggtt tcggcgaaat tgtgcgcata ttgctgctca ataacaccga
    33241 aattaccggc ggcccgaacg gaatcagtca gatcccgaaa ccgacactct tcggactcga
    33301 gttcagccgt accgctcgtg aaggcggctg ggacacgttc agtaatttct ttggcctgaa
    33361 atacgatccc tccgatcgtg tcatcttcct ctacctggtg gcgttgctgc tggtggtgct
    33421 aagcctgttt gtcattaacc gcctgctgcg gatgccgctg gggcgtgcgt gggaagcgtt
    33481 gcgtgaagac gagatcgcct gccgttcgct gggcttaagc ccgcgtcgta tcaagctgac
    33541 tgcctttacc ataagtgccg cgtttgccgg ttttgccgga acgctgtttg ctgcgcgcca
    33601 gggctttgtc agcccggaat ccttcacctt tgctgaatcg gcgtttgtac tggcgatagt
    33661 ggtgctcggc ggtatgggat cgcagtttgc ggtgattctg gcggcaattt tgctggtggt
    33721 atcgcgcgaa ttgatgcgtg atttcaacga atacagcatg ttaatgctcg gtggtttgat
    33781 ggtgctgatg atgatctggc gtccgcaggg cttgctgccc atgacgcgcc cgcaactgaa
    33841 gctgaaaaac ggcgcagcga aaggagagca ggcatgagtc agccattatt atctgttaac
    33901 ggtctgatga tgcgcttcgg cggcttgctg gcggtgaaca acgtcaatct tgaactgtac
    33961 ccgcaggaga tcgtctcgtt aatcggccct aacggtgccg gaaaaaccac ggtttttaac
    34021 tgcctgaccg gattctacaa acccaccggc ggcactattt tactgcgtga tcagcatctg
    34081 gaaggtttac cggggcagca aattgcccgc atgggcgtgg tgcgtacctt ccagcatgtg
    34141 cgtctgttcc gtgaaatgac ggtgattgaa aacctgctgg tggcgcagca tcaacaactg
    34201 aaaaccgggc tgttctctgg cctgttgaaa acgccttctt tccgtcgcgc ccagagcgaa
    34261 gcgctcgacc gcgccgccac ctggcttgag cgcattggtt tgctggaaca cgccaaccgt
    34321 caggcgagta acctggccta tggtgaccaa cgccgtctgg agattgcccg ctgcatggtg
    34381 acgcagccgg agattttaat gctcgacgaa ccggcggcag gtcttaaccc aaaagaaacc
    34441 aaagagctgg atgagctgat tgccgaactg cgcaatcatc acaacaccac tatcttgttg
    34501 attgaacacg atatgaagct ggtgatgggg atttcagacc gaatttatgt ggtcaatcag
    34561 gggacgccac tggcaaacgg tacgccggag caaatccgta ataacccgga cgtgatccgt
    34621 gcctatttag gtgaggcata agatggaaaa agtcatgttg tcctttgaca aagtcagcgc
    34681 ccactacggc aaaatccagg cgctgcatga ggtgagcttg catatcaatc agggcgagat
    34741 tgtcacgctg attggcgcga acggggcggg gaaaaccacc ttgcttggta cgttatgcgg
    34801 cgatccgcgc gccaccagcg ggcgaattgt gtttgatgat aaagacatta ccgactggca
    34861 gacggcgaaa atcatgcgcg aagcggtagc gattgtcccg gaagggcgtc gcgtcttctc
    34921 gcggatgacg gtggaagaga acctggcgat gggcggcttc tttgccgaac gtgaccagtt
    34981 tcaggagcgc ataaagtggg tgtatgagct gtttccacgt cttcatgagc gtcgtgttca
    35041 gcgggcgggc accatgtccg gcggtgaaca gcagatgctg gcgattggtc gcgcgctgat
    35101 gagcaacccg cgtttgttgt tgcttgatga gccatcgctc ggtcttgcgc cgattatcat
    35161 ccagcaaatt ttcgacacca tcgagcagct gcgcgagcag gggatgacta tcttcctcgt
    35221 cgagcagaac gccaaccagg cgctaaagct ggcggatcgc ggctacgtgc tggaaaacgg
    35281 ccatgtggtg ctctccgata ccggcgacgc gctgctggcg aacgaagcag tgagaagtgc
    35341 gtatttaggc gggcaataac acgttgattg ataaggagtc aaaagactcc tttgagacag
    35401 gtgacaaatg taaaattgcc tgatgcgctg cgcttatcag gcctactggg tgagttgcaa
    35461 tatattgaat ttgcacgatc ttgtaggccg gataaggcgt ttacgccgca tccggcatga
    35521 aacgatgagt aatctgtaga gtttgattca gaccttctat tttccgctta tccgtgcccc
    35581 atctcccatt ttccctcacc cacgccgtca ccgccttgtc atctttctga caccttacta
    35641 tcttacaaat gtaacaaaaa ggttattttt ctgtaattcg agcatgtcat gttaccccgc
    35701 gagcataaaa cgcgtgaatt cgcgcattcg gtacaacaag agagataaac gatgaaaccg
    35761 ttacattata cagcttcagc actggcgctc ggactggcgt taatggggaa tgcacaggca
    35821 gtgacgacca ttccgttctg gcattctatg gaaggggaac tgggtaaaga ggtggattct
    35881 ctggcccaac gttttaacgc cgaaaatccg gattacaaaa ttgtaccgac ctataaaggc
    35941 aactacgaac agaatttaag cgcggggatt gccgcatttc gtaccggcaa cgctccggct
    36001 attttgcagg tttatgaagt tggcaccgcc accatgatgg catcgaaagc cattaaaccg
    36061 gtatatgacg tgtttaaaga ggcggggatt caattcgatg agtcgcagtt tgtgccgacg
    36121 gtttccggtt actactccga cagcaaaacg ggccacttac tctcccagcc attcaacagc
    36181 tcgacccccg ttctctatta caacaaagac gccttcaaga aagcaggatt agacccggaa
    36241 caaccgccga aaacctggca ggatctggcg gactatgccg cgaaactgaa agcctccggt
    36301 atgaagtgcg gctacgccag cggctggcag ggctggatcc aactggaaaa ctttagcgcc
    36361 tggaacggtc tgccgtttgc cagcaaaaac aacggctttg acggcacgga cgcggtgctg
    36421 gagttcaaca agccggagca ggtgaaacac atcgccatgc tcgaggagat gaacaagaag
    36481 ggcgacttca gctacgtcgg tcgtaaggat gaatccaccg agaagttcta taacggtgat
    36541 tgcgcgatga ccaccgcctc ttccggttct ctcgctaata ttcgtgagta cgccaaattt
    36601 aactatggcg taggcatgat gccttacgac gccgatgcga aagatgcacc gcaaaacgcc
    36661 attatcggcg gggccagcct gtgggtgatg cagggcaaaa ataaagaaac gtataccggc
    36721 gtggcgaagt tcctcgactt cctcgcgaag ccagaaaacg ctgccgagtg gcatcagaaa
    36781 accggttatc tgccaatcac caaagcagcg tatgacctga cccgtgagca gggcttttat
    36841 gagaaaaacc caggggcgga taccgcgacg cgtcagatgc tgaataagcc gccgttgccg
    36901 ttcaccaaag ggctgcgtct gggcaacatg ccgcagatcc gcgtgattgt ggatgaagag
    36961 ctggagagcg tgtggaccgg taagaagaca ccacagcagg cactggatac cgccgttgag
    37021 cgtggaaatc agttgctgcg ccgctttgag aaatcgacga agtcttaatc agtgtaatgt
    37081 cggatgcgtt tcgcttatct gacctggcat cgcgtgtagg ccggataagc gaagcgcatc
    37141 cggcacagtt caggaattaa ccgtaatgtc atcatcccgt ccggtgttcc gctcgcgctg
    37201 gctgccttat ctgctggtcg cgccgcagct catcatcacc gttatctttt ttatctggcc
    37261 tgcgggcgaa gcgttgtggt actcgctaca aagcgtcgat ccgtttggtt tctccagcca
    37321 gtttgtcggc ctggataact tcgtcgcgct gtttcatgac agctactatc tcgactcctt
    37381 ctggacgacg ataaaattca gcacctttgt caccgtcagc ggtttgctgg tgtcgctgtt
    37441 cttcgcggcg ctggtggagt acatcgtgcg cggcagccgt ttctatcaaa ccttaatgtt
    37501 gctgccttac gccgtggctc ccgccgttgc cgccgtattg tggatcttcc tgtttaaccc
    37561 cggtcgcggg ctgatcaccc attttctcgc ggagttcggc tacgactgga accacgcgca
    37621 aaacagcggt caggcaatgt ttctggtggt gtttgcctca gtatggaagc aaatcagcta
    37681 caacttcctg ttcttctatg ccgcgctgca atccattccc cgttcgttga tcgaagccgc
    37741 agccatcgac ggtgcagggc cgattcgccg cttctttaag attgcgctgc cgcttatcgc
    37801 cccggtgagt ttcttcctgc tggtagtgaa cctggtgtat gccttcttcg acaccttccc
    37861 ggtgatcgac gccgccacgt ccggcgggcc ggttcaggcc accacgacgc tgatttataa
    37921 gatctaccgc gaaggtttta ccggactgga tctggcttcg tctgccgcac agtcggtggt
    37981 gttgatgttc ctcgtcatcg tgctgacggt ggtgcagttc cgctatgttg aaagcaaggt
    38041 gcgttaccaa tgattgagaa ccgtccgtgg ctgacgatat tcagccatac catgctgatc
    38101 ctcgggatcg cggtgatcct cttcccgctg tacgtggcgt ttgtcgcggc gacgctggat
    38161 aaacaggccg tctatgccgc gccgatgacg ctcatccccg gcacacatct gctggaaaac
    38221 atccacaaca tctgggtgaa cggggtaggc acgaatagcg cgccgttctg gcggatgttg
    38281 cttaacagct ttgtgatggc gttcagcatt acgctcggca aaattaccgt ctcgatgctc
    38341 tcggcatttg ccattgtctg gtttcgtttt ccgctacgta acctcttctt ctggatgatt
    38401 tttatcaccc tgatgctgcc ggttgaagta cgtatcttcc cgacggtgga agtcatcgcc
    38461 aacctgcaga tgctcgacag ctacgccggt ttaacgctgc cgctgatggc ctcggcgacc
    38521 gctactttcc tgttccgcca gttctttatg acgctgccgg atgagctggt ggaagccgcg
    38581 cggatcgacg gcgcatcgcc aatgcgcttc ttttgcgaca tcgtttttcc gctctccaaa
    38641 actaatctgg cggcgctgtt tgtgatcacc tttatctacg gctggaatca gtatttgtgg
    38701 ccgttgttga ttattaccga cgttgatctc ggcaccaccg tggcagggat caaagggatg
    38761 atcgccactg gcgaaggcac cacggaatgg aactcggtaa tggcggcgat gttgttaacg
    38821 cttatccctc cggtggtgat tgttttagtg atgcagcgtg ccttcgtgcg cggcctggtc
    38881 gatagtgaga aataagatgg caggactgaa attacaggca gtaaccaaaa gctgggatgg
    38941 caaaacccag gtgattaaac cactgaccct tgatgtggcg gatggcgaat ttatcgtgat
    39001 ggtcgggccg tctggctgcg gaaaatcgac gctgctgcgc atggttgccg ggctggagcg
    39061 ggtgacggaa ggcgatatct ggatcaacga ccagcgcgtg accgaaatgg agccgaaaga
    39121 tcgcgggatt gcgatggtgt tccagaacta cgcgctttat ccgcatatga gcgtcgaaga
    39181 aaacatggcg tgggggctga aaattcgcgg catgggcaag cagcaaatag ccgagcgcgt
    39241 taaagaagcg gcgcgcattc tggagctgga tggcctgctt aagcgccgcc cgcgcgagct
    39301 atccggcggt cagcgtcagc gtgtggcgat gggtcgcgcg attgtgcgcg atccggcggt
    39361 gttcctgttt gatgagccgc tctctaacct cgatgccaag ctgcgcgtgc agatgcgcct
    39421 tgagctgcaa cagctgcacc gtcgcctgaa aacgacttca ctctacgtta ctcacgatca
    39481 ggttgaagcg atgacgctcg cccagcgagt aatggtgatg aacggcggcg ttgccgaaca
    39541 gattggcaca ccagttgaag tctacgaaaa gcccgccagc ctgtttgtag cgagttttat
    39601 cggcagtccg gcgatgaacc tgctgacagg ccgcgtgaat aacgaaggca cgcatttcga
    39661 actggacggc ggtattgagc tgccgctaaa cggtggctac cgtcagtatg ccgggcgtaa
    39721 aatgactctc ggcattcgcc cggaacatat tgcgctaagc tcgcaggcag aaggcggcgt
    39781 accgctggtg atggacacgc tggagatcct cggcgcagat aacctggcgc acggacgctg
    39841 gggcgagcag aagctggtgg tgcgactggc gcatcaggag cgcccgacgg caggcagcac
    39901 gctgtggctg catctggcgg aaaatcagct gcatcttttt gatggtgaaa caggacaacg
    39961 agtatgagta actggcctta tccccgcatc gtcgctcatc gtggcggcgg taagctggcc
    40021 ccggaaaaca ccctggcggc aatcgacgtc ggggcaaaat acggtcataa gatgatcgaa
    40081 tttgacgcga agttatcgaa agatggcgag atcttcctgc tccatgacga caatctcgaa
    40141 cgcaccagca acggctgggg cgtcgcgggt gaactgaact ggcaggattt actgcgcgtg
    40201 gatgcgggca gttggtacag caaagcgttt aaaggtgagt cgctgccgtt gctttcgcag
    40261 gtggcggaac gctgtcgcga acacgggatg atggcgaata tcgaaatcaa acccaccact
    40321 ggcactggac cattaacggg caaaatggtg gcgctggcgg cacgccaact gtgggccggt
    40381 atgacgccgc cgctgctgtc atcgtttgag attgatgctt tagaagctgc acagctggcg
    40441 gcaccggaac tgccgcgtgg tttattgctt gatgagtggc gcgacgactg gcgcgaactg
    40501 accgcgcggc tgggctgcgt ctctattcat ctcaatcata agttactcga taaagtgcga
    40561 gtgatgcagt tgaaagacgc cggactgcgg attctggttt ataccgtcaa taaaccccag
    40621 cgcgcggcag agttgctgcg ctggggcgtg gattgcatct gtaccgatgc gattgacgtg
    40681 attggtccga actttacggc ccaatagttt tcaacggaat gtcaggctgc ggcgtgctgg
    40741 gctgattcag catgtcgccg tttctcctct ccggcagcat atgctgctga ttcaacgaac
    40801 tatccggatt acggttgctg tttaacatcc cgccgttggt gttgggcagc atttgctgcc
    40861 gggcgggatt tcgctccccc ggctgtgact gcaacacccg ctgagaatta ttgtttatct
    40921 ggttttctaa atgctgctgt tgcaactgag tttgcgtttt cagttgctga ttcagcatcc
    40981 ctttttgctg gatttgctga gtctgcatct gggtttgcat ccgctgctgg ctgggtatct
    41041 gataccccgg ctggttaggg ttgttcagag tattaatggg ctgtgcaaag ccgacaaacg
    41101 gcaggagtgc cgtaagaatc agaagtcgtt tcatcgcgta tcctcctctg aagatatcct
    41161 ttaagtttac tcgcttcccg acaaaacgat gattaattca gagttatata ccaggcttag
    41221 ctggggttgc cccttaatct ctggagaata acgatgataa aaccgacgtt tttacgccgg
    41281 gtggccattg ctgctctgct ctcaggaagt tgttttagcg ccgccgccgc gcctcctgca
    41341 ccgcccgtct cgtatggtgt ggaggaagat gtcttccacc cggtacgcgc gaaacaggga
    41401 atggtagcgt ctgtggacgc cactgccact caggtggggg tggatattct caaggagggc
    41461 gggaatgccg ttgatgccgc cgtggcggtg ggctacgcgc tggcggtaac gcatccgcag
    41521 gcagggaatc tgggcggtgg tggttttatg ctgatccgct cgaaaaatgg caataccacc
    41581 gctatcgatt tccgcgaaat ggcacccgct aaagcgaccc gcgatatgtt ccttgatgac
    41641 cagggcaacc cggacagcaa aaaatcactc acttcgcatc tggcttccgg cacaccaggt
    41701 acggtcgctg gtttctcgct ggcgttggat aagtacggca ctatgccgtt gaacaaagtg
    41761 gtgcagcccg cgttcaaact ggcacgcgat ggttttatcg ttaacgatgc tttggcagac
    41821 gatctcaaaa cctacggtag cgaagtgttg ccgaatcacg aaaacagcaa agctatcttc
    41881 tggaaagagg gcgaaccgct gaaaaagggc gacacgctgg tgcaggcgaa cctggcaaag
    41941 agcctggaga tgattgctga aaacggcccg gacgaattct ataaaggcac gattgcggaa
    42001 cagatcgccc aggagatgca gaaaaacggt ggcttgatca ctaaagaaga tttagcggcc
    42061 tataaagcgg tcgaacgcac tccgataagc ggcgattatc gcgggtatca ggtttactcc
    42121 atgccaccgc catcctccgg cgggatccat atcgtacaaa tcctcaatat tctggaaaac
    42181 ttcgatatga agaaatacgg ctttggcagc gccgatgcga tgcaaatcat ggcagaagcg
    42241 gagaaatacg cctacgccga ccgctcggaa tatcttggcg acccggattt tgtcaaagtc
    42301 ccgtggcagg cactgaccaa taaagcctat gccaaatcca ttgccgatca aattgatatc
    42361 aacaaagcga agccgtccag tgagattcgc cctggcaagc ttgcgcctta tgagagtaat
    42421 caaactaccc attactctgt agtggataaa gacggtaacg cggtggcggt gacctatacg
    42481 ctgaacacca ccttcggtac gggtattgtc gcgggcgaga gcggtattct gcttaataac
    42541 cagatggatg atttctccgc caaaccaggc gtaccgaacg tttacgggct ggtgggcggt
    42601 gatgccaacg ccgtcgggcc gaacaaacgc ccgctgtcgt cgatgtcgcc gaccattgtg
    42661 gtgaaagacg gtaaaacctg gctggttacc ggtagcccag gcggtagccg gatcatcact
    42721 acagtgctgc aaatggtggt gaatagcatc gattatggca tgaacgtcgc cgaagcgacc
    42781 aatgcgccgc gtttccacca tcagtggttg ccggacgagc tgcgtgtcga aaaagggttt
    42841 agcccggata cgctcaagct gctggaagca aaaggtcaga aagtggcgct gaaagaggcg
    42901 atgggcagta cacaaagcat tatggttggg ccggacggtg agttgtacgg cgcatccgac
    42961 ccgcgctcgg tggatgattt aacggcgggg tactaaggtt agcggccctc ttcgtgggaa
    43021 gagggctatt ttgtcagggc aagccgaagg tagccttctt tatttcgtaa tcctgtagat
    43081 attcttccag cggctttgcc gttcccagag aatgaatttc accactgtct ttatcaataa
    43141 taaaaggtgc gttaccagct aagcgcgcgg cctcatctcc agtttcgaga aattctcgtg
    43201 cttcgaaaca gaaataccag ccctggctaa agcgtccatg tagagtaatg acgaccggga
    43261 gatctgcatc atcaaggtaa tggttcgctt tcgcgaatgc gtcgtgataa gtaatcataa
    43321 ttataataaa tattattgtt gagtgttata ttattattcc tgtgtgatac attgagcaaa
    43381 gacgcgttca tcttcgtcaa agatattatt aaatgctggt tttgaaaaaa gttcatcgga
    43441 taagatatag tcacgaaata agtatctgct
//
LOCUS       DAZFQD010000023        42931 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.023, whole genome shotgun sequence.
ACCESSION   DAZFQD010000023 DAZFQD010000000
VERSION     DAZFQD010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 42931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42931
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.023"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..121
                     /locus_tag="ACHKTW_001359"
     CDS             <1..121
                     /locus_tag="ACHKTW_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS element protein dsORF-f3"
                     /protein_id="HFO6462417.1"
                     /translation="YESPTSTHNDIMGGGGMSYAYEYDVKYNICHLRKKSRMR"
     gene            complement(208..883)
                     /locus_tag="ACHKTW_001360"
                     /pseudo
     CDS             complement(208..883)
                     /locus_tag="ACHKTW_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(1028..1636)
                     /gene="yiiX"
                     /locus_tag="ACHKTW_001361"
     CDS             complement(1028..1636)
                     /gene="yiiX"
                     /locus_tag="ACHKTW_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HFO6462418.1"
                     /translation="MKNRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVIRNKKPYVFEAVGPVKYTPLKQWIAHGEKGKYVVRRVEGGLSVEQQQK
                     LAQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            complement(1820..2137)
                     /gene="metJ"
                     /locus_tag="ACHKTW_001362"
     CDS             complement(1820..2137)
                     /gene="metJ"
                     /locus_tag="ACHKTW_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HFO6462419.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            2414..3574
                     /gene="metB"
                     /locus_tag="ACHKTW_001363"
     CDS             2414..3574
                     /gene="metB"
                     /locus_tag="ACHKTW_001363"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HFO6462420.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLRRFLSGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            3577..6009
                     /gene="metL"
                     /locus_tag="ACHKTW_001364"
     CDS             3577..6009
                     /gene="metL"
                     /locus_tag="ACHKTW_001364"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HFO6462421.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEYSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPGKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(6148..6282)
                     /locus_tag="ACHKTW_001365"
     CDS             complement(6148..6282)
                     /locus_tag="ACHKTW_001365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6462422.1"
                     /translation="MKEKPKGVNRKMTNDGEEKSGKMKKLQDGKKKKIRYAKQTGLQI
                     "
     gene            6358..7248
                     /gene="metF"
                     /locus_tag="ACHKTW_001366"
     CDS             6358..7248
                     /gene="metF"
                     /locus_tag="ACHKTW_001366"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HFO6462423.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            7577..9757
                     /gene="katG"
                     /locus_tag="ACHKTW_001367"
     CDS             7577..9757
                     /gene="katG"
                     /locus_tag="ACHKTW_001367"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HFO6462424.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            9851..10756
                     /gene="yijE"
                     /locus_tag="ACHKTW_001368"
     CDS             9851..10756
                     /gene="yijE"
                     /locus_tag="ACHKTW_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HFO6462425.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYTRHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVLALALVSRKKKEAVSVKSI"
     gene            complement(10783..11400)
                     /gene="yijF"
                     /locus_tag="ACHKTW_001369"
     CDS             complement(10783..11400)
                     /gene="yijF"
                     /locus_tag="ACHKTW_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HFO6462426.1"
                     /translation="MKASLALLSLLTAFTSHSLKSPAVPPTVVQIQANTNLAIADGAR
                     QQIGSTLFYDPAYMQLTYPGGDVPQERGVCSDVVIRALRSQKVDLQKLVHEDMAKNFA
                     EYPQKWQLKRPDSNIDHRRVPNLETWFTRHDKTRPISKNPSDYQAGDIVSWRLDNGLA
                     HIGVVSDGFARDGTPLVIHNIGAGAQEEDVLFSWRMVGHYRYFVK"
     gene            complement(11675..12778)
                     /gene="gldA"
                     /locus_tag="ACHKTW_001370"
     CDS             complement(11675..12778)
                     /gene="gldA"
                     /locus_tag="ACHKTW_001370"
                     /EC_number="1.1.1.6"
                     /EC_number="1.1.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
                     /protein_id="HFO6462427.1"
                     /translation="MDRIIQSPGKYIQGADVINRLGEYLKPLAERWLVVGDKFVLGFA
                     QSTVEKSFKDAGLVVEIAPFGGECSQNEIDRLRGIAETAQCGAILGIGGGKTLDTAKA
                     LAHFMGVPVAIAPTIASTDAPCSALSVIYTDEGEFDRYLLLPNNPNMVIVDTKIVAGA
                     PARLLAAGIGDALATWFEARACSRSGATTMAGGKCTQAALALAELCYNTLLEEGEKAM
                     LAAEQHVVTPALEHVIEANTYLSGVGFESGGLAAAHAVHNGLTAIPEAHHYYHGEKVA
                     FGTLTQLVLENAPVEEIETVAALSHAVGLPITLAQLDIKEDVPAKMRIVAEAACAEGE
                     TIHNMPGGATPDQVYAALLVADQYGQRFLQEWE"
     gene            complement(12789..13451)
                     /gene="fsa"
                     /locus_tag="ACHKTW_001371"
     CDS             complement(12789..13451)
                     /gene="fsa"
                     /locus_tag="ACHKTW_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFO6462428.1"
                     /translation="MELYLDTANVAEVERLARIFPIAGVTTNPSIIAASKESIWEVLP
                     RLQKAIGDEGILFAQTMSRDAQGMVEEAKRLRDAIPGIVVKIPVTSEGLAAIKMLKKE
                     GITTLGTAVYSAAQGLLAALAGAKYVAPYVNRVDAQGGDGIRTVQELQALLEMHAPES
                     MVLAASFKTPRQALDCLLAGCESITLPLDVAQQMLNTPAVESAIEKFEHDWNAAFGTT
                     HL"
     gene            complement(13463..15964)
                     /gene="ptsP"
                     /locus_tag="ACHKTW_001372"
     CDS             complement(13463..15964)
                     /gene="ptsP"
                     /locus_tag="ACHKTW_001372"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFO6462429.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLRTDRK
                     GNAKSALALIGTDTLAGDNCQLLISGADEQEAHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALGNLPAAKGVDAEQSALE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLREHLLAGVSAGLSCAEAIVTS
                     ANHFCEEFARSSSSYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQPAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQRVWLTQQARTADGIRIEIAANIAHSVE
                     AQAAFGNGAEGVGLFRTEMLYMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGG
                     DKPVDYLNIPAEANPFLGYRAVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSM
                     EEILWVKEKLAEAKQQLRNEHIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSN
                     DLTQYLLAVDRDNAKVTRHYNSLNPAFLRALDYAVQAVHRQGKWIGLCGELGAKGSVL
                     PLLVGLGLDELSMSAPSIPAAKARMAQLDSRECRKLLNQAMACRTSLEVEHLLAQFRM
                     TQQDAPLVTAECITLESDWRSKEEVLKDMTDNLLLAGRCRYPRKLEADLWAREAVFST
                     GLGFSFAIPHSKSEHIEQSTISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIF
                     SRLARRIMHEEFRNALVNAASADAIASLLQHELEL"
     gene            16273..17352
                     /gene="frwC"
                     /locus_tag="ACHKTW_001373"
     CDS             16273..17352
                     /gene="frwC"
                     /locus_tag="ACHKTW_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HFO6462430.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIVGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            17367..17687
                     /gene="frwB"
                     /locus_tag="ACHKTW_001374"
     CDS             17367..17687
                     /gene="frwB"
                     /locus_tag="ACHKTW_001374"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HFO6462431.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            17738..20035
                     /gene="pflD"
                     /locus_tag="ACHKTW_001375"
     CDS             17738..20035
                     /gene="pflD"
                     /locus_tag="ACHKTW_001375"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFO6462432.1"
                     /translation="MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVILRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATSTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAANCT
                     DAQRREELLTIAEISRHNAEHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQASLTQGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMVFEQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRAFTQLKLQHIQFNVVNADTLREAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            20001..20879
                     /gene="pflC"
                     /locus_tag="ACHKTW_001376"
     CDS             20001..20879
                     /gene="pflC"
                     /locus_tag="ACHKTW_001376"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312908.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HFO6462433.1"
                     /translation="MTSSAGQRISCNVVETRRNDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVNMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLNIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVTT
                     MREMAERAGFQVTVGG"
     gene            20881..21222
                     /gene="frwD"
                     /locus_tag="ACHKTW_001377"
     CDS             20881..21222
                     /gene="frwD"
                     /locus_tag="ACHKTW_001377"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HFO6462434.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRVDVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(21209..22060)
                     /gene="yijO"
                     /locus_tag="ACHKTW_001378"
     CDS             complement(21209..22060)
                     /gene="yijO"
                     /locus_tag="ACHKTW_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO6462435.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVAAGGWNFPQWKTPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTERSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(22209..23942)
                     /gene="eptC"
                     /locus_tag="ACHKTW_001379"
     CDS             complement(22209..23942)
                     /gene="eptC"
                     /locus_tag="ACHKTW_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HFO6462436.1"
                     /translation="MHSTEVQAKPLFSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWAASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQDVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLSAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            complement(24125..26776)
                     /gene="ppc"
                     /locus_tag="ACHKTW_001380"
     CDS             complement(24125..26776)
                     /gene="ppc"
                     /locus_tag="ACHKTW_001380"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HFO6462437.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYEHNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            complement(27128..28279)
                     /gene="argE"
                     /locus_tag="ACHKTW_001381"
     CDS             complement(27128..28279)
                     /gene="argE"
                     /locus_tag="ACHKTW_001381"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HFO6462438.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASIGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGVNAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPLPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELITQVIHHFCWH"
     gene            28433..29437
                     /gene="argC"
                     /locus_tag="ACHKTW_001382"
     CDS             28433..29437
                     /gene="argC"
                     /locus_tag="ACHKTW_001382"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HFO6462439.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVDLPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKSGVTQAQVAQVLQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            29445..30221
                     /gene="argB"
                     /locus_tag="ACHKTW_001383"
     CDS             29445..30221
                     /gene="argB"
                     /locus_tag="ACHKTW_001383"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HFO6462440.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRHAEQLPALFNGMPMGTRILA"
     gene            30282..31655
                     /gene="argH"
                     /locus_tag="ACHKTW_001384"
     CDS             30282..31655
                     /gene="argH"
                     /locus_tag="ACHKTW_001384"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HFO6462441.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLSELQKFSHVIGEDVYPILSLQSCLDKRAAKGGVSPQQVAQAIA
                     FAQARLE"
     gene            32154..33367
                     /gene="manD"
                     /locus_tag="ACHKTW_001385"
                     /pseudo
     CDS             32154..33367
                     /gene="manD"
                     /locus_tag="ACHKTW_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-mannonate dehydratase ManD"
     gene            33434..34717
                     /locus_tag="ACHKTW_001386"
     CDS             33434..34717
                     /locus_tag="ACHKTW_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000382182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO6462442.1"
                     /translation="MDVDVSTSVAGNKPQRIRRIQTVTLVLLFMAGIVNFLDRSSLSV
                     AGEAIRGELGLSATEFGVLLSAFSLSYGFSQLPSGILLDRFGPRIVLGAGLIFWSLMQ
                     ALTGMVNSFSHFILMRIGLGIGEAPFMPAGVKSITDWYAQKERGTALGIFNSSTVIGQ
                     AIAPPALVLMQLAWGWRTMFVIIGVAGILVGICWYAWYRNRAQFVLTDEERTYLSAPV
                     KPRPQLQFSEWLALFKHRTTWGMILGFSGVNYTGWLYIAWLPGYLQAEQGFSLAKTGW
                     VAAIPFLAAAVGMWVNGIVVDRLAKKGYDLAKTRKTAIVCGLMMSALGTLLVVQSSSP
                     AQAVAFISMALFCVHFAGTSAWGLVQVMVSETKVASIAGIQNFGSFVFASFAPIVTGW
                     VVDTTHSFNLALVIAACVTFTGALCYFFIVKDRIE"
     gene            34969..35886
                     /gene="oxyR"
                     /locus_tag="ACHKTW_001387"
     CDS             34969..35886
                     /gene="oxyR"
                     /locus_tag="ACHKTW_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HFO6462443.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVPMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(35869..37269)
                     /gene="sthA"
                     /locus_tag="ACHKTW_001388"
     CDS             complement(35869..37269)
                     /gene="sthA"
                     /locus_tag="ACHKTW_001388"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003957 - NAD(P)+ transhydrogenase
                     (Si-specific) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HFO6462444.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIEGCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            37590..38240
                     /gene="fabR"
                     /locus_tag="ACHKTW_001389"
     CDS             37590..38240
                     /gene="fabR"
                     /locus_tag="ACHKTW_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HFO6462445.1"
                     /translation="MMGIRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            38240..38599
                     /gene="yijD"
                     /locus_tag="ACHKTW_001390"
     CDS             38240..38599
                     /gene="yijD"
                     /locus_tag="ACHKTW_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HFO6462446.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMPVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(38639..39739)
                     /gene="trmA"
                     /locus_tag="ACHKTW_001391"
     CDS             complement(38639..39739)
                     /gene="trmA"
                     /locus_tag="ACHKTW_001391"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030697 - tRNA
                     (uracil(54)-C5)-methyltransferase activity, S-adenosyl
                     methionine-dependent [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HFO6462447.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IELDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQCETIFVDPPRSGLDSETEKMVQAYPRILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     regulatory      39853..40043
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            40108..41952
                     /gene="btuB"
                     /locus_tag="ACHKTW_001392"
     CDS             40108..41952
                     /gene="btuB"
                     /locus_tag="ACHKTW_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015420 - ABC-type vitamin B12 transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HFO6462448.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTANRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRVEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRVTLLGDYAHTHGYDVVAYGNTGTQAQTDNDGFL
                     SKTLYGALEHNFTDAWSGFVRGYGYDNRTNYDAYYSPGSPLLDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHYGRYDSSATLDEMKQYTVQWANNVIVGHGSIGAG
                     VDWQKQTTTPGTGYVENGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKAPNLGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     gene            41897..42754
                     /gene="murI"
                     /locus_tag="ACHKTW_001393"
     CDS             41897..42754
                     /gene="murI"
                     /locus_tag="ACHKTW_001393"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFO6462449.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEEFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
BASE COUNT         9784 a        10697 c        11801 g        10649 t
ORIGIN      
        1 ttatgaaagc ccaacatcaa cccacaatga cataatgggg ggcgggggaa tgtcatatgc
       61 ctatgaatat gatgtaaaat ataatatatg tcatttgaga aaaaagagca gaatgaggta
      121 aaaatctttg gctgtgtatt cagtctggta atcatccaag gaaagcttat cagaagggtg
      181 atgactccaa ttagtagaac atatattttc gataaaagca ccgatgactt tttcatgaag
      241 ttctacagaa cgcgagaaac agatggttct gcgggccaga cgtttgattc gggttctcag
      301 ggtcagattg ttacgctcaa tgtgctgagt aaaaatctta ccagtcagat gcttctcctt
      361 cggtaactct ctagcgtaac taccccattc gtcagtggtc aacatgccga tggtaaaagg
      421 gtaagcagcg cccgcaattc acgacaggtt tcgtcagtcc gggggccaaa agtgtaggcc
      481 agaacaccac cagttttcgt gttgtacacg taccagagcc agtgctgtcg cgctttactg
      541 ccgacaaagc tccattgctg actaagtttg cagatgagtg ccacatcagc atgagcgacc
      601 ggggatgacg ttattcttcg tggcgagagt tttttaaagt tcggatgact gtattaatgc
      661 cgattttcag cgtacgggca gtatcgcgaa ccccggcacc gttgaaagtc atttcagcga
      721 tttggtcttt aactcccggt ttacggggct cgtaacagta actgagctga aatacccggt
      781 gacagtcacg gccgcggaat cgatcatgtc tttttgggtt ctgcccgtga cggtaaacct
      841 gaacggactg gcaacggggg caatgaaggt taactgtgac catgaaacaa ccttaaaagc
      901 gagtattata tccttaattc aactaattgg aggcatcacc gtttaccgga gcgacagctt
      961 gcggaaaggt cgtgcaacta caaagcaaca aacagactac ggcgggaatt atttttttca
     1021 tcatccatta ccacgaaaac agcggccact ctttggcaac cgtggtgagt tgtggcgcgt
     1081 cgaaaacggc ttgtggtgaa actaccgttt cctcaagcgg aatattttta ccgtagcgtt
     1141 ctttgagctt cgcctggacc agcgggttgc tgagatcaaa ctctttcaac ttctgctgtt
     1201 cgcccacgcg cattcccagc gcgttctggt aaaccttcca caccacttcc gaacagtact
     1261 ggcgatcgtc gctccatgag aagctgaaat catacggttt accgagataa cgttttgccg
     1321 tttgcgccag tttttgctgt tgttcaacac tcagtccgcc ttcaacgcgg cgaacgacgt
     1381 atttgccctt ttcaccatgg gcgatccact gcttgagcgg ggtgtatttc accgggccga
     1441 ccgcttcaaa aacgtagggc tttttgttgc gtatcaccag cataccggtg tggctgtaat
     1501 cggaatgggt tgccagttgg atcgctttac tttgcgatga gcgagagatt tgaaagatga
     1561 tgtcgccagt ttgcggctgc caggcaaagg caggtacaga aaccagcagg ctgaggatca
     1621 gcagcctgtt tttcatagtt aaacgtccat gtataaaaag cggtgggtcg cagacaacgt
     1681 gctcgttgtt tatgccggat gcggcgtgaa cgccttatcc ggcctacaag ttcgtgcaaa
     1741 ttcaataaat tgcaatatga cgtaggcctg ataagcgtag cgcatcaggc ggttccactc
     1801 cgcgccgctc ttttccgctt tagtattccc acgtctccgg gttaatcccc atctcacgca
     1861 tgatctcttt tgccgcttcc gggatttcgt cgctgcgctc tttacgcaga tcggcatcat
     1921 ccggcaaagg ttgcccggta aaggcatgca gaaacgcttc gcacagcagc tcgctgttgg
     1981 tagcgtgacg caggttgttc acctgacgac gcgtgcgttc atcggtgagg atttttaaca
     2041 ccttaagagg aatggaaacc gtaatctttt tgacttgttc actcttcttg ccgtgctcag
     2101 cgtatgggct gatatattcg ccgctccatt cagccatgag atacttaatc ctcttcgtca
     2161 ataaattgag accagaccac acagttaatg tgggcactgg ccgtaaaccc gcatagttta
     2221 ccgtacaggc gctaccgtta catcgggtaa tccacctgct ggcgtgataa tgcatataat
     2281 tttaacggct atttgggatt tgctcaatct atacgcaaag aagtttagat gtccagatgt
     2341 attgacgtcc attaccacaa tgtttactct ggtgcctgac atttcaccaa taaagcccag
     2401 ggaacatcat cacatgacgc gtaaacaggc caccatcgca gtgcgtagcg ggttaaatga
     2461 cgacgaacag tatggttgcg ttgtcccacc catccatctt tccagtacct ataactttac
     2521 cggatttaat gaaccgcgcg cgcatgatta ctcgcgtcgt ggcaacccaa cgcgcgatgt
     2581 ggttcagcga gcactggcag aactggaagg cggtgctggt gcagtactta ctaataccgg
     2641 catgtccgcg attcacctgg taacgaccgt ctttttgaaa cctggcgatc tgctggtagc
     2701 gccgcacgac tgctacggcg gtagctatcg cctgttcgac agcctggcga aacgtggttg
     2761 ctatcgcgtg ttgtttgttg atcaaggcga tgaacaggca ttacgggcag cgctggcaga
     2821 aaaacccaaa ctggtactgg tagaaagccc aagtaatcca ttgttacgcg tcgtggatat
     2881 tgcgaaaatc tgccatctgg caagggaagt cggggcggtg agcgtggtgg ataacacctt
     2941 cttaagcccg gcattacaaa atccgctggc attaggtgcc gatctggtgt tgcattcatg
     3001 cacgaaatat ctgaacggtc actcagacgt ggtggccggc gtggtgattg ctaaagaccc
     3061 ggacgttgtc actgaacttg cctggtgggc aaacaatatt ggcgtgacgg gcggcgcgtt
     3121 tgacagctat ctgctgctac gcgggttgcg aacgctggtg ccgcgcatgg agttggccca
     3181 gcgtaacgcg caggcgattg tgaaatatct gcaaacccag ccgttggtga aaaaactgta
     3241 tcacccgtcg ctgccggaaa atcaggggca tgaaattgcc gcgcgtcagc aaaaaggctt
     3301 tggcgcaatg ttgagttttg aactggatgg cgatgagcag acgctgcgtc gtttcctgag
     3361 cgggctgtcg ttgtttacgc tggcggaatc attaggggga gtggaaagtt taatctctca
     3421 cgccgcaacc atgacacatg caggcatggc accagaagcg cgtgctgccg ccgggatctc
     3481 cgagacgctg ctgcgtatct ccaccggtat tgaagatggc gaagatttaa ttgccgacct
     3541 ggaaaatggc ttccgggctg caaacaaggg gtaaaaatga gtgtgattgc gcaggcaggg
     3601 gcgaaaggtc gtcagctgca taaatttggt ggcagtagtc tggctgatgt gaagtgttat
     3661 ttgcgtgtcg cgggcattat ggcggagtac tctcagcctg acgatatgat ggtggtttcc
     3721 gccgccggta gcaccactaa ccagttgatt aactggttga aactaagcca gaccgatcgt
     3781 ctctctgcgc atcaggttca acaaacgctg cgtcgctatc agtgcgatct gattagcggt
     3841 ctgctacccg ctgaagaagc cgatagcctc attagcgctt ttgtcagcga ccttgagcgc
     3901 ctggcggcgc tgctcgacag cggtattaac gacgcagtgt atgcggaagt ggtggggcac
     3961 ggtgaagtgt ggtcggcacg cctgatgtct gcggtactta atcaacaagg gctgccagcg
     4021 gcctggcttg atgcccgcga gtttttacgc gctgaacgcg ccgcacaacc gcaggttgat
     4081 gaagggcttt cttacccgtt gctgcaacag ctgctggtgc aacatccggg caaacgtctg
     4141 gtggtgaccg gatttatcag ccgcaacaac gccggtgaaa cggtgctgct ggggcgtaac
     4201 ggttccgact attccgcgac acaaatcggt gcgctggcgg gggtttctcg cgtaaccatc
     4261 tggagcgacg tcgccggggt atacagtgcc gacccgcgta aagtgaaaga tgcctgcctg
     4321 ctgccgttgc tgcgtctgga tgaggccagc gaactggcgc gcctggcggc tcccgttctt
     4381 cacgcccgta ctttacagcc ggtttctggc agcgaaatcg acctgcaact gcgctgtagc
     4441 tacacgccgg atcaaggttc cacgcgcatt gaacgcgtgc tggcctccgg tactggtgcg
     4501 cgtattgtca ccagccacga tgatgtctgt ttgattgagt ttcaggtgcc cgccagtcag
     4561 gatttcaaac tggcgcataa agagatcgac caaatcctga aacgcgcgca ggtacgcccg
     4621 ctggcggttg gcgtacataa cgatcgccag ttgctgcaat tttgctacac ctcagaagtg
     4681 gccgacagtg cgctgaaaat cctcgacgaa gcgggattac ctggcgaact gcgcctgcgt
     4741 caggggctgg cgctggtggc gatggtcggt gcaggcgtca cccgtaaccc gctgcattgc
     4801 caccgcttct ggcagcaact gaaaggccag ccggtcgaat ttacctggca gtccgatgac
     4861 ggcatcagcc tggtggcagt actgcgcacc ggcccgaccg aaagcctgat tcaggggctg
     4921 catcagtccg tcttccgcgc agaaaaacgc atcggcctgg tattgttcgg taagggcaat
     4981 atcggttccc gttggctgga actgttcgcc cgtgagcaga gcacgctttc ggcacgtacc
     5041 ggctttgagt ttgtgctggc aggtgtggtg gacagccgcc gcagcctgtt gagctatgac
     5101 gggctggacg ccagccgcgc gttagccttc ttcaacgatg aagcggttga gcaggatgaa
     5161 gagtcgttgt tcctgtggat gcgcgcccat ccgtatgatg atttagtggt gctggacgtt
     5221 accgccagcc agcagcttgc tgatcagtat cttgatttcg ccagccacgg tttccacgtt
     5281 atcagcgcca acaaactggc gggagccagc gacagcaata aatatcgcca gatccacgac
     5341 gccttcgaaa aaaccgggcg tcactggctg tacaatgcca ccgtcggtgc gggcttgccg
     5401 atcaaccaca ccgtgcgcga tctgatcgac agcggcgata ctattttgtc gatcagcggg
     5461 atcttctccg gcacgctctc ctggctgttc ctgcaattcg acggtagcgt gccgtttacc
     5521 gagctggtgg atcaggcgtg gcagcagggc ttaaccgagc ctgatccgcg tgacgacctc
     5581 tccggcaaag acgtaatgcg caagctggta attctggcgc gtgaagcggg ttacaacatt
     5641 gaaccggacc aggtacgtgt ggaatcgctg gtgcctgctc attgcgaagg cggcagcatc
     5701 gaccatttct ttgaaaatgg cgatgaactt aacgagcaga tggtgcaacg gctggaagcg
     5761 gcccgcgaaa tggggctggt gctgcgctac gtggcgcgtt tcgatgccaa cggcaaagcg
     5821 cgtgtgggcg tagaggcggt gcgtgaagac catccgctgg catcactgct gccgtgcgat
     5881 aacgtctttg ccatcgaaag ccgctggtat cgcgacaacc cgctggtgat ccgtgggcct
     5941 ggcgcggggc gcgacgtcac cgccggggcg attcagtcgg atatcaaccg gctggcacag
     6001 ttgttgtaat ttcagaaatt taataatgcc cggtactcat gttttcgggt ttatggtttc
     6061 taatgaaata tattgaatta tcataggatt aggccggatt aagcgtttac gacgaatccg
     6121 gcaagaagca ataagtacat ggttagttta tatttgcagt ccggtttgct ttgcataccg
     6181 gattttcttt ttcttaccat cctgaagttt tttcatcttc cctgattttt cctcaccatc
     6241 attggtcatt tttcggttga cgcccttcgg cttttccttc atctttacat ctggacgtct
     6301 aaacggatag atgtgcacaa cacaacatat aactacaagc gattgatgag gtaaggtatg
     6361 agcttttttc acgccagcca gcgggatgcc ctgaatcaga gcctggcaga agtccagggg
     6421 caaattaacg tttcgttcga gtttttcccg ccgcgtacca gtgaaatgga gcagaccctg
     6481 tggaactcca tcgatcgcct tagcagcctg aaaccgaagt ttgtatcggt gacctatggc
     6541 gcgaactccg gcgagcgcga ccgtacgcac agcattatta aaggcattaa agatcgcact
     6601 ggtctggaag cggcaccgca tcttacttgc attgatgcga cgcccgacga gctgcgcacc
     6661 attgcacgcg actactggaa taacggtatt cgtcatatcg tggcgctgcg tggcgatctg
     6721 ccgccgggaa gtggtaagcc ggaaatgtat gcttctgacc tggtgacgct gttaaaagaa
     6781 gtggcagatt tcgatatctc cgtggcggcg tatccggagg ttcacccgga agcaaaaagc
     6841 gctcaggcgg atttgctcaa cctgaaacgc aaagtcgatg ccggagccaa ccgcgcgatt
     6901 actcagttct tctttgatgt cgaaagctac ctgcgttttc gtgaccgctg cgtatcggcg
     6961 ggtattgatg tggaaattat tccgggaatt ttgccggtct cgaactttaa acaagcgaag
     7021 aaatttgccg atatgaccaa cgtacgtatt ccggcgtgga tggcacaaat gtttgacggg
     7081 ctggacgacg atgccgaaac ccgcaaactg gtgggagcga atattgccat ggatatggtg
     7141 aagattttaa gccgtgaagg agtgaaagat ttccacttct atacgcttaa ccgcgctgaa
     7201 atgagttacg cgatttgcca tacgctgggt gttcgacctg gtttataaat agtgtggctt
     7261 ttgtgaaaat cacacagtga tcacaaattt taaacagagc acaaaatgct gcctcgaaat
     7321 gagggcggga aaataaggtt atcagccttg ttttctccct cattacttga aggatatgaa
     7381 cctaaaaccc ttttttataa agcatttgtc cgaattcgga cataatcaaa aaagcttaat
     7441 tacgatcaat ttgatctaca tctctttaac caacaatatg taagatctca actatcgcat
     7501 ccgtggatta attcaattat aacttctctc taacgctgtg tatcgtaacg gtaacactgt
     7561 agaggggagc acattgatga gcacgtcaga cgatattcat aacaccacag ccactggcaa
     7621 atgcccgttc catcagggcg gtcacgacca gagcgcgggg gcgggcacaa ccactcgcga
     7681 ctggtggcca aatcaactcc gtgttgacct gttaaaccaa cattctaatc gttctaaccc
     7741 actgggtgag gactttgact accgcaaaga attcagcaaa ttagattact acggcctgaa
     7801 aaaagatctg aaagccctgc tgacagaatc tcagccgtgg tggcctgccg actggggcag
     7861 ttacgccggt ctgtttattc gtatggcctg gcacggcgcg ggtacttacc gttcaatcga
     7921 tggacgcggc ggcgcgggtc gtggtcagca acgttttgca ccgctgaact cttggccgga
     7981 taacgtaagc ctcgataaag cgcgtcgcct gttgtggcca atcaaacaga aatatggtca
     8041 gaaaatctcc tgggccgacc tgtttatcct cgcaggtaac gtggcgctgg aaaactccgg
     8101 cttccgtacc ttcggttttg gtgccggtcg tgaagacgtc tgggaaccgg atctggatgt
     8161 taactggggt gatgaaaaag cctggctgac tcaccgtcat ccggaagcgc tggcgaaagc
     8221 accgctgggt gcaaccgaga tgggcctgat ttacgttaac ccggaaggcc cggaccacag
     8281 cggcgaaccg ctttctgcgg cagcagctat ccgcgcgacc ttcggcaaca tgggcatgaa
     8341 cgacgaagaa accgtggcgc tgattgcggg tggtcatacg ctgggtaaaa cccacggtgc
     8401 cggtccgaca tcaaatgtag gtcctgatcc agaagctgca ccgattgaag aacaaggttt
     8461 aggctgggcg agcacttacg gcagcggcgt tggcgcagat gccattacct ctggtctgga
     8521 agttgtctgg acccagacgc cgacccagtg gagcaactat ttcttcgaga acctgttcaa
     8581 atatgagtgg gtacagaccc gcagtccggc tggcgcaatc cagttcgaag cggtagacgc
     8641 accggaaatt atcccggatc cgttcgatcc gtcgaagaaa cgtaaaccga cgatgctggt
     8701 gaccgacctg acgctgcgtt ttgatcctga gttcgagaag atctctcgtc gtttcctcaa
     8761 cgatccgcag gcgttcaacg aagcctttgc ccgtgcctgg ttcaaactga cgcacaggga
     8821 tatggggccg aaatctcgtt acatcggacc ggaagtaccg aaagaagatc tgatctggca
     8881 agatccgctg ccgcagccga tctacaaccc gaccgagcag gacattatcg atctgaaatt
     8941 cgcgattgcg gattctggtc tgtctgttag tgagctggta tcggtggcct gggcatctgc
     9001 ttctaccttc cgtggtggcg acaaacgcgg tggtgccaac ggtgcgcgtc tggcattaat
     9061 gccgcagcgc gactgggatg tgaacgccgc agccgttcgt gctctgcctg ttctggagaa
     9121 aatccagaaa gagtctggta aagcctcgct ggcggatatc atcgtgctgg ctggtgtggt
     9181 tggtgttgag aaagccgcaa gcgccgcagg tttgagcatt catgtaccgt ttgcgccggg
     9241 tcgcgttgat gcgcgtcagg atcagactga cattgagatg tttgagctgc tggagccaat
     9301 tgctgacggt ttccgtaact atcgcgctcg tctggacgtt tccaccaccg agtcactgct
     9361 gatcgacaaa gcacagcaac tgacgctgac cgcgccggaa atgactgcgc tggtgggcgg
     9421 gatgcgtgta ctgggtgcca acttcgatgg cagcaaaaac ggcgtcttca ctgaccgcgt
     9481 tggcgtattg agcaatgact tcttcgtgaa cttgctggat atgcgttacg agtggaaagc
     9541 gactgacgaa tcgaaagagc tgttcgaagg tcgtgaccgt gaaaccggcg aagtgaaata
     9601 cacagccagc cgtgcggatc tggtatttgg ttctaactct gtcctgcgtg cggtagcgga
     9661 agtttacgcc agcagcgatg cccacgagaa gtttgttaaa gacttcgtgg cggcatgggt
     9721 gaaagtgatg aacctcgacc gtttcgacct gctgtaatct gaccttcttc agcgactgtc
     9781 tttcaggcag tcgctgaagt ttctttaccg gcgtatagtg tccacaggaa aactacacac
     9841 tggatctctc atgtctgccg caggaaagag caacccactg gcaatcagtg gcctggttgt
     9901 gctcacactt atctggagtt atagctggat tttcatgaag caagtcacca gctacatcgg
     9961 tgccttcgac tttaccgcct tacgctgcat tttcggcgct ctcgttttat tcatcgtcct
    10021 tttattacgt ggtcgcggaa tgcgcccgac accgtttaaa tacaccttag ccattgccct
    10081 gttacaaacc tgcgggatgg ttggcctggc gcagtgggcg ctggtcagcg gcggagcggg
    10141 gaaggtggcg atcctaagct ataccatgcc gttctgggtg gtgattttcg ccgcgttgtt
    10201 tctcggtgaa cgcctgcgac gtgggcaata tttcgcgata ctgattgccg ctttcggctt
    10261 atttttggtg ttgcagccgt ggcaactcga tttctcttca atgaaaagtg ccatgctggc
    10321 aatcctctcc ggcgtcagtt ggggggcgag cgcgattgtt gctaaacgcc tatacacccg
    10381 tcatccgcgc gtggatttat tatcgttaac atcctggcag atgctctatg cggcgctggt
    10441 gatgagtgtg gtcgctttac tggtgccgca acgtgaaatt gactggcagc ccaccgtgtt
    10501 ctgggcgctg gcctacagtg cgattctggc gacggcgctg gcgtggagct tatggttgtt
    10561 tgtattgaaa aacttgcctg ccagtattgc cagcttaagc acgctggccg ttcccgtttg
    10621 cggcgtactc ttttcctggt ggctgctcgg tgagaatccg ggggccgttg aaggtagcgg
    10681 tattgtgctg attgtgctgg cactggcgct ggtgagccgt aagaaaaaag aagctgtcag
    10741 tgtaaaaagc atctgaattt tttcttcatg tggggcgatc gcttatttaa caaaataacg
    10801 ataatgcccc accatccgcc agctaaacag cacatcttct tcctgcgcgc ctgcgccaat
    10861 gttatgtatc accagcggcg taccgtcgcg ggcgaagcca tctgacacca ccccaatatg
    10921 tgccagtccg ttatccagtc gccaggagac aatatcgccc gcttgatagt cactggggtt
    10981 cttgctgatg gggcgtgttt tatcgtggcg ggtaaaccag gtttccagat taggcacccg
    11041 acggtgatcg atgttgctgt ccgggcgctt taactgccat ttttgcgggt actcggcaaa
    11101 attcttcgcc atatcttcgt gcaccagttt ctgcaaatcg accttctggc tgcgcaatgc
    11161 acggatcacc acatcggaac ataccccgcg ttcttgcggg acatcaccgc caggataggt
    11221 aagctgcata tacgccggat cgtaaaatag cgtgctgccg atttgctgtc tggcaccgtc
    11281 tgcgatggca aggttggtat tggcctggat ttgtaccacg gttggtggaa cggcgggaga
    11341 ttttaaggag tggctggtaa atgccgttag caggctgagc agcgccagtg aagctttcat
    11401 ctggttcgtc cgtgaaaata agagtcacag attcgacctt ccgggctaac gattgttctg
    11461 tggagaaaat gtccagaagc ggataaaaaa ctcctgaaca acgtgggagt ttcagattac
    11521 tggcacagag tgccggatgc ggagtgaacg ccttatccgg cctacaaaag cacgcatatt
    11581 caatatattg cagagatttc gtaggcttga taaacgtagc gcatcaggca attttgcgtt
    11641 caaactcccg gacaagccgg gagtttggag taggttattc ccactcttgc aggaaacgct
    11701 gaccgtactg gtcggctacc agcagagcgg cgtaaacctg atctggcgtc gcgccgccag
    11761 gcatgttgtg aatggtttca ccttctgcac atgccgcttc tgccacaatt cgcattttcg
    11821 ccgggacatc ttctttaata tccagttgag cgagagttat tggcaaacct accgcatggc
    11881 taagtgcagc tacggtttcg atttcctcaa ccggtgcgtt ttccagaacc agctgcgtca
    11941 gcgtaccaaa tgccactttt tcaccgtgat aatagtgatg cgcctccggg atagcggtca
    12001 ggccgttatg caccgcgtga gccgccgcca gcccgccgct ttcaaaacca acgccgctca
    12061 aataggtgtt cgcttcaatc acgtgctcca gcgccggggt tactacatgc tgttcggcgg
    12121 caagcatcgc tttttcgcct tcttccagca gggtgttgta gcacagttca gccagtgcca
    12181 gcgccgcctg ggtgcacttg ccgcccgcca tggtggtcgc gccgctacga gagcaagcac
    12241 gcgcttcaaa ccaggttgcc agcgcatcgc cgatacccgc tgctaacaga cgtgcaggtg
    12301 cgcccgcgac gattttggtg tcgacaatga ccatattcgg gttatttggc aacagcagat
    12361 agcggtcaaa ctcaccctca tcggtgtaga taacagacaa tgcgctgcac ggtgcatcgg
    12421 tagaggcgat agtcggtgcg atcgctaccg gaacacccat gaaatgtgcc agtgctttgg
    12481 cagtatcgag ggtttttccg ccaccgatac cgagaattgc gccacactgc gcagtctccg
    12541 cgatgccacg cagacggtcg atctcatttt gcgaacattc accgccaaac ggcgcaattt
    12601 ctactaccag tccagcatct ttaaagcttt tctcgacagt ggattgagca aaacctaaaa
    12661 caaatttgtc acccaccact aaccagcgtt ctgccagcgg cttcaggtat tcgcccagac
    12721 gattaatcac atcagcgccc tggatgtatt tacccggtga ttgaataatg cggtccataa
    12781 ttgctccttt agagatgagt agtgccaaat gcggcattcc agtcgtgttc gaacttctct
    12841 atagctgact ctaccgcagg ggtgttgagc atttgttgcg ctacatctaa gggcagggtg
    12901 atggattcac atcctgccag taaacagtcc agcgcctgac gcggcgtttt aaagctggcc
    12961 gccagcacca tgctttctgg tgcgtgcatt tctaacagcg cttgcagctc ctgaaccgta
    13021 cgaatgccgt ccccgccctg ggcatctacg cggttaacat acggagcaac gtattttgcc
    13081 cctgccagcg cggccagtaa cccttgtgca gcactgtaca ctgcggtgcc aagcgtagta
    13141 ataccctctt ttttcagcat cttaatggcg gccagacctt cggaagtcac cgggattttc
    13201 accacaatgc ccggaatggc gtcgcgcagg cgcttcgctt cttccaccat accctgcgcg
    13261 tcacgactca tagtctgagc aaacagaatg ccctcatcac caatcgcttt ttgcagacgc
    13321 ggcagaactt cccatatgga ctccttgctg gcagcgataa tgctcgggtt agttgtcacc
    13381 ccggcaatgg ggaatatgcg tgccagacgt tcgacttctg cgacgttagc ggtgtccaga
    13441 tacagttcca tgatgtttcc ttttacagtt ccagttcatg ttgcagcagg ctggcgatag
    13501 cgtcggcaga ggcggcgtta accagcgcgt tacggaattc ttcgtgcatg atgcggcgag
    13561 cgaggcgcga gaaaatgcgc atgtgctgat cgcccgcagc gtgtttgttc agggttaaca
    13621 taatgatgaa ttgcgcttca tcatcgcccc agcgcaccgg cgcttgcaga cgcgccacgc
    13681 tgatggtgga ttgctcaatg tgttctgatt tgctgtgtgg aatggcaaaa ctaaagccca
    13741 gaccggtaga gaaaacggcc tcgcgcgccc ataagtcggc ttccagttta cgcggatagc
    13801 ggcagcggcc cgccagcagc aggttatcgg tcatgtcttt gagcacttct tctttgctgc
    13861 gccagtcact ttccagcgtg atgcactcgg cggtgaccag cggtgcgtcc tgttgggtca
    13921 tgcggaattg cgccagcagg tgttccactt ccagcgaagt acggcaggcc attgcctggt
    13981 tgagcaactt gcggcactca cggctatcaa gttgcgccat ccgcgctttc gccgccggaa
    14041 ttgatggtgc gctcatgctg agttcatcca gccctaagcc gaccagcaac ggcagcacgg
    14101 aacctttcgc tcccagctca ccgcacagac caatccattt gccctggcga tgcaccgcct
    14161 gcacggcgta atcgagcgcc cgcaagaatg ccggattcag gctgttgtag tgacgagtaa
    14221 ccttagcgtt atcgcgatcc actgccagca gatattgcgt caggtcatta ctaccaatac
    14281 taaagaaatc aatctcttcg cagcattgat cgatgatgaa catcaccgac ggtacttcca
    14341 gcataatgcc aagctggatc ttctcatcaa acggaatgtg ttcgttacgc agttgctgtt
    14401 tggcttccgc cagtttttct ttcacccata agatctcttc cattgaggag atcatcggga
    14461 tcatgatttt caggctgccg tgagcggagg cgcgcaggat cgaccgtagt tgtgtggtga
    14521 acagcgaggc gtattcttca taaatacgca cagcgcgata accgaggaac gggttagctt
    14581 ctgccggaat gttcagataa tcaacgggtt tgtcgccgcc aatgtccata gtgcgcacaa
    14641 taatgctgcg tccgttggca gactccagcg cctgacaaaa aatgttgtac agctcgcttt
    14701 cgcccggtgc actggtgcga tccatataga gcatttcagt gcggaacaaa ccaacgcctt
    14761 ccgcaccatt gccgaatgcg gcctgcgctt ccacggagtg agcgatgtta gcggcaattt
    14821 caatgcggat accgtcagcg gtacgggctt gttgggtcag ccagacacgc tgttgctcac
    14881 gcagggcgtc ctgtacgcgg gcttcttgct gataataacg agctacggct tctcctggct
    14941 caaccacaat cgccccagcg ttgccgtcga tataaatcgt ttgatgctgc cacggagtaa
    15001 gggcatcaat atctacacca accagtgtcg gaatgttgaa cgaacgggca agaattacgg
    15061 tatgtgaggt ggtgccgcca cttttcagta acagaccttt gaggtgattt ttatccagtt
    15121 cgaggaactg gctgggagtc agttcatcag ccatacaaat ggcgggctgc gtcagtttgc
    15181 ccggtgccgg gaagcgttgc tcaccgtaga tttgctggag taactggaag cagacgtcgc
    15241 gtacgtccag ggcacgttct tgcaggtagc tgctgctgga acgggcgaac tcttcgcaaa
    15301 agtgattcgc actggtgaca atcgcttcgg cgcagcttaa tccggcgctg acgcctgcca
    15361 gtaaatgttc gcgcagggaa gtatcgccag ccagggatcg gtgcgcttcc agaatcgcgc
    15421 tggtggcacc gtcgctatcc agcagacgaa actcaatgtt tttcagtacc agcgtcaggc
    15481 cgttttccag tgcggattgc tcggcgtcaa cgcctttggc tgcgggaaga ttacccagcg
    15541 cattgagatc taaagaagag atcggcgtca gaatgccgcc tgcactaccg ctgcacacgg
    15601 tgcgggcgcg gataatttgc ggattcagat tggtcagtga aaccggcagt ggttccagtt
    15661 cgtcagattt aacttccgcc agcggcgcat cgcagtgggg gaattcatcg cgcagccatt
    15721 ggcttaaacg ctggtgcgct tcctgttcgt cggccccgga aatcagtaac tggcagttat
    15781 cgcccgccag cgtatcggtg ccaatcagcg caagggcgct tttggcgttg cccttgcggt
    15841 cagtgcgcag gttatgccac tcaatttgtg atgaaaaagt attacacagc gtttcaacgt
    15901 ggcttgccgg acgtgcatgt acgccgttag gtagctcaca aataaattcc acaatcaggg
    15961 ccattgcctc tctcccggta cggttttctg tttacaggat aagagcgcac ggcaacggcc
    16021 tgccatgtga caaatctgcc aaaagctgga caaatgtaat gtaaccgtca atttgcgacg
    16081 cgtctcacaa gacgctgttt tgcggcatgc ttccggttta tcgcaagtta tgaggcggat
    16141 cgcatttttg tactgatatt acaaaagtcc agtaaatggc ctttttatcc actgtttgct
    16201 ccgcctgcga ttgcctattg tttcctccat caaaaatatg gacatgggct ttcgcaagcc
    16261 cgggagcagc ttatgaatga gttggtgcag atcctgaaaa atacccgtca gcatttaatg
    16321 acgggcgttt cacacatgat tcccttcgtg gtatcgggcg gtattttgct ggcggtttcc
    16381 gtcatgttgt atggcaaagg cgcagtgccg gatgccgtag ccgatccaaa tctgaaaaaa
    16441 ctgtttgata tcggcgttgc gggcttgacg ctgatggtgc ctttcctcgc agcgtacatc
    16501 ggttattcca ttgcagagcg ttctgcgctg gctccgtgcg ctatcggtgc ctgggttggt
    16561 aacagctttg gtgcgggctt ctttggtgca ctgatcgccg ggattatcgg cggcatcgtg
    16621 gtgcattacc tgaagaaaat tccggtgcat aaagttctgc gctcggtgat gcctatcttc
    16681 attattccta tcgtcggcac actgattacc gcaggcatca tgatgtgggg cttgggcgag
    16741 cctgtagggg cgttgaccaa cagcctgact cagtggcttc aggggatgca gcagggcagc
    16801 attgttatgc tggcggtgat catgggtctg atgctggcgt tcgatatggg cggtccggtt
    16861 aacaaagtgg cctatgcctt catgctgatt tgcgttgctc agggtgttta taccgtggtg
    16921 gccatcgctg ccgtgggtat ttgtatcccg ccgctgggga tggggctggc gacgctgatt
    16981 ggtcgtaaaa atttctccgc agaagagcgc gaaaccggca aagcggcact ggtgatgggc
    17041 tgtgttggcg ttactgaagg ggcgattcct ttcgccgctg ccgatccgct gcgtgtcatt
    17101 ccttccatca tggtcggttc agtttgtggt gcagtaactg cggcgctggt cggtgcgcag
    17161 tgctatgcag gctggggtgg tctgattgtg ctgccggtgg ttgaaggcaa gctgggttat
    17221 atcgcagcag tggctgtcgg agcagtggtg acggctgttt gtgtgaacgt gctgaaaagt
    17281 ctggcgcgta aaaatgggtc ttcgactgat gaaaaagaag acgacctgga tttggatttt
    17341 gaaattaatt aattgaggaa cgagccatga cgaaaattat tgcagtaacc gcatgtcctt
    17401 caggtgttgc ccatacctat atggcagcag aggcgctgga aagtgccgcg aaagcgaaag
    17461 gctgggaagt gaaagtagaa acccaggggt caattggtct ggaaaatgaa ctgactgcgg
    17521 aagatgtggc gagcgctgat atggtgattc tgaccaaaga tatcggcatc aagtttgaag
    17581 agcgttttgc tggcaagacc atcgtgcgcg tcaacatcag cgatgcagta aaacgtgctg
    17641 acgccattat gagcaaaatt gaagctcacc tggcgcaaac cgcttaagtt cttctcccgc
    17701 tcgcaagggc gggttcgctt tcccacagga gttcctcatg acgaatcgta tctctcgcct
    17761 caaaactgca ctgtttgcca atacccgtga aatctcgctg gagcgggcgc tgctttatac
    17821 cgccagccat cggcaaaccg aaggcgaacc ggtgatattg cgccgggcga aagcaacagc
    17881 gtatatcctt gaacatgttg aaatttcgat tcgtgatgaa gaactgattg ccggtaaccg
    17941 caccgtaaaa ccgcgcgctg ggattatgtc gccggaaatg gacccttact ggctgctgaa
    18001 agagctggat caattcccga cgcgtccgca ggaccgcttt gctatcagtg aagaagataa
    18061 acgtatctac cgtgaagagt tgttcccgta ctgggaaaaa cgttcgatga aagatttcat
    18121 caacgggcag atgacagatg aagtaaaagc cgcgaccagc acgcagattt tcagcatcaa
    18181 ccagacagat aaaggccagg ggcacattat tattgattac ccacgcctgc tgaatcacgg
    18241 gctgggggag ctggtggcgc agatgcagca acattgtcag caacagccgg agaatcactt
    18301 ttatcaggcc gcgctgttac tgctggaagc ctcgcagaaa cacattctgc gttacgctga
    18361 actggcggaa acaatggcgg caaactgcac agatgcccag cgtcgcgaag aactgctgac
    18421 tattgcggag atctcccgcc ataacgccga acataagccg cagacgttct ggcaggcgtg
    18481 ccagttattc tggtacatga acatcattct gcaatacgaa tccaacgcca gttcgctatc
    18541 gttggggcgc ttcgaccagt atatgttgcc gttctatcag gcatctttaa cccagggcga
    18601 agatccggcg ttcctgaaag aactactcga atctttatgg gtgaaatgca acgacatcgt
    18661 gctgttgcgc tccaccagca gcgcgcgtta tttcgcaggt ttcccgaccg gctataccgc
    18721 actgctcggc gggttaaccg agaacggacg tagcgcggtg aacgtgcttt cgttcctttg
    18781 ccttgacgct tatcaaagcg tgcaattacc gcaaccgaac ctcggcgtgc gcactaacgc
    18841 cttgatcgac acgccgttcc tgatgaaaac cgccgaaacc attcgcctcg gcaccggtat
    18901 tccgcaaatc tttaacgatg aagtggtggt accagcgttc ctcaaccgtg gcgtttcgct
    18961 ggaagatgcg cgcgactatt ccgtagtggg ctgtgtggaa ttatctattc ccggcagaac
    19021 ctacggcttg catgacatcg cgatgtttaa tctgctgaaa gtgatggaaa tctgcctgca
    19081 tgaaaatgaa ggcaatgctg cgctgactta tgaaggttta ctggagcaga tccgcgccaa
    19141 gatcagccac tacatcaccc tgatggttga gggcagcaat atttgtgata tcggccatcg
    19201 cgactgggca cctgtaccgc tgctctcgtc ttttatcagc gattgtctgg aaaaaggtcg
    19261 cgatattacc gatggcggcg cgcgttataa cttctccggc gtacagggga tcggtatcgc
    19321 caacctgagc gattctctcc atgcgttgaa agggatggtt tttgagcaac agcgtttaag
    19381 ttttgacgaa ttgctgtcgg tattaaaagc caactttgca acgccagaag gcgaaaaagt
    19441 ccgtgctcgc ttaattaacc gcttcgagaa atacggtaac gatatcgacg aggtggataa
    19501 catcagcgcc gaactgttac gccactactg caaagaagtg gaaaaatacc agaacccgcg
    19561 cggcggttac ttcacgccgg gatcgtatac cgtttctgct cacgtcccgt tgggatcggt
    19621 ggttggcgcg acgccagacg gtcgttttgc cggggaacaa ctggcagacg gcggcttgtc
    19681 acctatgctg ggccaggacg cacaagggcc aacagcggta ctgaagtcag tcagtaagct
    19741 cgataatacg ctgctgtcta acggtacgtt gctgaacgtg aaattcactc cggcgaccct
    19801 ggaaggtgaa gcgggattac gcaaactggc cgacttctta cgggcgttta cccagcttaa
    19861 gttgcagcat attcagttta acgtggtgaa cgccgacacg ttgcgggaag cgcaacagcg
    19921 cccacaagat tatgccgggc tggtggtgcg cgttgccgga tacagcgcct tctttgtcga
    19981 actgtcgaag gagatccagg atgacatcat ccgccggaca gcgcatcagc tgtaacgttg
    20041 tggaaacgcg ccgcaatgat gtggcgcgca ttttcaacat tcagcgttat tcactgaatg
    20101 acggcgaggg cattcgtacg gtggtctttt ttaaaggctg tccgcatctt tgcccgtggt
    20161 gtgctaatcc ggagtcgatc tccggcaaaa tccagacggt acgcagagag gcgaaatgtc
    20221 tgcactgtgc gaaatgtttg cgtgatgcgg atgaatgccc ctccggtgcg tttgaacgga
    20281 ttggtcgcga tatcagcctt gacgctctgg aacgggaagt gatgaaagat gacattttct
    20341 ttcgcacgtc cggcggcggc gtcacgcttt ctggcggcga agtgttaatg caggcggagt
    20401 ttgctacccg ttttttacag cgactgcggc tgtggggtgt ctcatgtgcc attgaaactg
    20461 ccggagacgc gccggccagc aagctgttac cactggcgaa attgtgcgat gaagtgttgt
    20521 tcgatttaaa aattatggat gcgactcagg cgcgggatgt ggtgaatatg aacctgccac
    20581 gcgtgctgga gaatctgcgt ttgctggtga gtgagggcgt caacgtgatc ccgcgtttac
    20641 cgctgatccc tggtttcacg ctcagccggg agaatatgca gcaggcgctg gacgtgctga
    20701 tcccgctgaa tatcaggcag atccatctgt taccgtttca tcagtacggc gaaccgaaat
    20761 accgcctgct ggggaaaaca tggtcgatga aagaggtgcc tgcgccgtcg tcagccgatg
    20821 tgacaacgat gcgcgaaatg gcagaacggg ccggatttca ggttaccgtg ggaggttaaa
    20881 atggcatacc tggtggcagt aaccgcctgc gtaagcggcg tggcgcatac atatatggcg
    20941 gcggaacggc tggaaaagtt gtgccagtta gagaagtggg gcgtcagcat tgaaactcag
    21001 ggcgcgttgg gaacggagaa tcgtttagcg gacgaggata tccgtcgggt ggatgttgct
    21061 ctgttgatta cggatatcga gcttgccggt gccgagcgat ttgaacattg ccgctatgtg
    21121 caatgcagca tctacgcatt cctgcgtgag ccgcagcggg taatgagcgc ggtgcgcaaa
    21181 gtgctttctg caccgcagca aacccatctt attctggagt agtcggtttt tctgtcagtt
    21241 ggctgtggta ctgccggcga tattccgacg gcgagcgttc ggtgttttta cgaaacagac
    21301 ggcagaagta gttgctgtcg acaaagccgc aggcgtgcgc cacttctttt accttcaggt
    21361 catagccttt caataacgtc ttagcgtgct ccagtcgcgt gtgattcagg tattcgttaa
    21421 aaccaatggc ccccgttttt tgaaacaggt gcgagagata gtttggtgaa atatagaatg
    21481 cctgtgcgac agattcgcgg gtaagcgcgg aggcatagcg ttcgtcgata taatcgcgaa
    21541 tagcttcaaa tagtgcctgg ctgcgtgagg cggtctggat ttggctgccg agcaaatcgc
    21601 ggcagtggct aagcaggctg gctacgataa gccttgccgt ttgctgctcc tgcggctgca
    21661 tttgcatttc attgagcgtt tgtagcagaa aagaacctat acgtgggccg cgtcgggcga
    21721 cgtgttgctt cgccagattt tgatattgtt tgccatccca ttgcacgacg ctgaagccga
    21781 gttgctgttt gccaaacagc acgctaaagg tggtagcggg tgttttccat tgtgggaaat
    21841 tccagccacc agcggcaacg tacagcacat cgccgggaac taacgttgct ccagcgccgg
    21901 tatcaacaaa ctcaccttcc agcacaattt ccagccgtgg aaaatcgacc tgatacgcaa
    21961 gatcgggcac cgggccgtta ctgctggcaa agtaaatctg gcgcagggac agcgggccgt
    22021 tgatcaggcg ggagagcaga tagctgacgt cgtgatacat gttattccct taggtatgta
    22081 gacgggaaaa cagtaactgg cattattaaa aaaggccacc tgaaaagtgt ggcctgaggg
    22141 gagttcgatg taaacattgc ggcagtatcg catccggcaa atagcgccag acgcgataca
    22201 aaggtcattt actgattacc tacctggtcg ccatacggca gagtgtcgta atcgatcagc
    22261 gcgtttttct tgtacgggtt accaatccag cgggtagttt ctttgaactg cggattcacc
    22321 actgaacggg ttggatcgta accgtcgtaa gataagcccg ccaaatctga ccaggtgtgg
    22381 atcagttccg ccaggctgta tttacgatcg acatcctgtg agaaatcacg gggatgagtc
    22441 gcttgccatt tttccgacgt ccacagcagg aacggaatgg tgtacatgtg gcgcgtcggg
    22501 ttgtcttcgt tacgcccctg agttttgtgc ggcggcgtgt cgtaaacctc ttcaccgtgg
    22561 tcagagaagt aaaccaggaa tccgttcggg tctgccgctt taaagtcttt aatcaggctg
    22621 gcaaccacat gatcgttata caggttagcg ttgtcataat cgttatatga ttccagctct
    22681 tctgcgctta atcctggcgg aacatgatcg gtattgccat caaacttgcc ctgattttcc
    22741 gggtagcggt atttgtattt gatatgcgta cccagcagat gaacgatgat cagtttcttc
    22801 ggcgcagggt cattcagcac atcctggaac ggcttcagca cgttggtgtc gtattcacgc
    22861 gcactctgcg tacgttgctg gttcatgtag tactgcttgt cggtctggcg cgaaaatacc
    22921 gtcagcatgg tattgcgggc ggtcatcgtc tgctggttgg tgatccagaa ggttttataa
    22981 cccgcctgtt tcatcatgtt catcagcgac ggctgcgtca gatacagatc cgggttcttt
    23041 tcattggcaa aggtcagcgc ctgttgcagg atttcaatgg tgtacggacg agacgtaact
    23101 acgttattaa acacggtcag attcggatcg gttttatgca gcgcatccag ctccggcgtg
    23161 gtttcacgcg gataaccgta cagactcatg cgtccgcgct gggtcgactc gccaatcacc
    23221 agcactaaag tgcgcggttc gttacccgat tcatctttga aattagccag tggcggcaag
    23281 gcattatttt cattcagtaa ctttgttagc gagtttagtt gctgacgata ctgataatag
    23341 ccggtcagga attgccacgg tgcggcaggc tccatacgcg aggccaggtt atccaacgtt
    23401 ttctcaaacg gcttgttttt gataaacgta ttcatggcga tcggatgcag aatcaagcca
    23461 taaagcaggg caaaagagac aacataacgc cacggctttg gaatatagac cgggcgcagg
    23521 cgtgtccaca gcagaactgc caccgccgta taggccagcg cgataagcac aattttcagg
    23581 ctgaaatact ggcttaaata ctcgctggct tcgttggtgt tggtttcgaa catcacaaac
    23641 agaacgctct gcgagaactc ctgcccgtag atgacgtagt agcatagcgc cgccagagag
    23701 gccgcccata gcaccacgcc gattactgcg gcaataattt taatccgctt cggaaagagg
    23761 aataccggga tcaaccacag cgagctgaat aacagcgagt cgcgaatgcc gttggtgcca
    23821 ctataaccac tgatgtaaat aatggcctgt agcagagtag agaaaaacca aaagtagagc
    23881 agtgcccaac ccagggcttt ccagctaaaa aggggtttag cctggacttc tgtggaatgc
    23941 atagtaagaa cctgtcttga aaaaaatatc gccgaatgta acgacaattc cttaaggata
    24001 tctgaaggta tattcagaat ttgaataaaa tgcagacaga aatatattga aaacgagggt
    24061 gttagaacag aagtatttca gaaaaccctc gcgcaaaagc acgagggttt gcagaagagg
    24121 aagattagcc ggtattacgc atacctgccg caatcccggc aatagtgacc attaacgctt
    24181 gttcgacgcg aggatccggt tcctggcctt ctttttctgc ctggcgggag cggtgcagta
    24241 actcggcctg caatacgttc agcgggtcgg tgtaaatatt ccgtagctga atagactctg
    24301 caatccacgg cagatcggcc attagatggg aatcgttggc aatcgccagc accactttga
    24361 tgtcttcttc ttgcaggttg cgtaactctt tacctaacgg ccacagtgct ttgtctacca
    24421 ggcgttggtc atagtattcc gccagccaca ggtctgcttt ggcgaagacc atctccagca
    24481 tgccgagacg cgtcgagaag aatggccaat cgcggcacat ggcttccagc tcgctctgtt
    24541 tgccatcttc gaccactttt tgcagcgccg tacctgcacc cagccaggcg gggagcatca
    24601 ggcggttttg cgtccaggca aaaatccacg gtatggcgcg cagcgactcg acgccgccgg
    24661 ttgggcgacg tttcgccgga cgtgaaccca acggcagttt gcccaattct tgttccggcg
    24721 tagcggagcg gaagtaaggc acaaaatctt tgttttcacg tacgtagcca cggtagagat
    24781 cgcaggagat gactgacagt tcatccataa tgcgacgcca gctctctttc ggctcaggtg
    24841 gtggcagcag gttggcttcc agaatcgccc cggtgtacag cgacaggctg ctgacggtga
    24901 tttctggcag accatattta aagcggatca tctcgccctg ttcagtcacg cgcaggccac
    24961 ctttcaggct tcctggcggt tgtgacagca gcgccgcatg agcaggtgcg ccgccgcgac
    25021 caatggaacc gccgcgaccg tggaacaacg tcagctcaat acccgctttt tcgcaggttt
    25081 tgattaatgc atcctgtgcc tgatattgcg cccaggaagc tgccatcact cccgcatctt
    25141 ttgctgagtc ggaatagcca atcatcacca tctgtttgcc ctgaatcagg ccacgatacc
    25201 agtcaatatt gagcagctgg gtcatgacat cgttggcgtt gttcagatca tcgagggttt
    25261 caaacagcgg agcaaccggc atcgcaaacc cgatacccgc ttctttcagc agcaggtgga
    25321 cagccagtac gtcggacggc gttttcgcca tcgagatcac gtaggcggca atggagcctt
    25381 gcggcgcttc ggcaatcacc tggcaggtat cgagcacttc gcgcgtttcg gcgcttggtt
    25441 gccagttgcg cggcagaagc ggacgtttgg agttcagttc gcggatcagg aacgcctgtt
    25501 tgtcggcctc tgaccagctt tcgtagtcgc cgataccgag gtagcgggtc agctcgccca
    25561 gcgcttcggt atgacgcgtg ctctcctgac ggatatcaat acggaccagc ggtacgccga
    25621 aacatttcac gcggcgcagg gtgtcgagca gatcgccgtt ggcgataata cccatgccac
    25681 acgcctgaag tgactggtag caagcgtaga gcggttccca cagttcttcg ttttgtgtca
    25741 gcaggccttc tggttttggc agttcttcgc ctttcaggcg cgcttccagc catgcctgtg
    25801 tcgccatcag gcgagaacgc aggtttttca tcagatagcg atacggttct gcggcacctt
    25861 cttcgccaac cagcgccagc agttcagggg tcgcttcaac catcgacagt tcagaaacca
    25921 gcacctgaat atctttcagg aacaaatcgg tggctttcca gcggctgagt agcaggacgt
    25981 ggcgggtgat atcggcagtg acgttcgggt tgccgtcgcg gtcgccgccc atccacgaag
    26041 taaaacggac cggaacaaat tcgacgggca gtttgtagcc gaggttctct tccagttgtt
    26101 cgttcagttc gcgcaggtaa tttggtacgc cttgccacag gctgttttcc actacggcaa
    26161 agccccattt ggcttcatct accgggcttg gacgcagctt acggatttca tcggtatgcc
    26221 atgactgggc gattaactgg cgcaggcggc gcatcagctg gttgtgttcg tagtcagcga
    26281 tatctttgtt atcgagctgt tttaaacagg cgttcacttc caccattttg tggatcagtg
    26341 tacgacgggt aatttcggtt gggtgagccg tgaggaccag ttccagcgac agcgattcca
    26401 ctgctttttt gatggtgtct tcgctcagtt ccggctggtt tttcagttta cgcagggtgc
    26461 gggcgatcac ttccgggttg ctggcagctt cgcctttcgg cgaaatgctg tggtattgct
    26521 cggcggtgtt ggccaggttc aggaactgac taaacgcacg cgcaacgggc agcagctcgt
    26581 cgttcgacaa attttgtaag gtggtgagca actcctggcg gttagcatca ttgccagcgc
    26641 gtgaagattt cgacaactta cggatagttt ctacgcgttc aagaatgtgt tctcccaacg
    26701 catccttgat ggtttctccc agcactttgc cgagcatact gacattacta cgcaatgcgg
    26761 aatattgttc gttcatatta ccccagacac cccatcttat cgtttgatag ccctgtatcc
    26821 ttcacgtcgc attggcgcga atatgctcgg gctttgcttt tcgtcgtctt ttataaagcc
    26881 acgtaaaagc ggtgacgtca aatgctgcga aatcgcttca gcaaacgaat aaatagcagg
    26941 aatttacgcc attaaattca cgacgcttta aataagcgta acttatggaa atgttaaaaa
    27001 atcgccatac ataacaccaa aggtgtaggt cggataagat gcgcaagcat cgcatccgac
    27061 attattgcgg cacaggagtt tggcaacggt gccggatgcg gcgcgagcgc cttatccggc
    27121 ctacgtttta atgccagcaa aaatggtgaa ttacctgggt tatcagttcg cgggtgggct
    27181 tgataaaccg tgtttccaga tattcatcag gttgatgagc ctgattaatt gagccaggcc
    27241 ccaacaccag cgtcgggcat aacgtttgaa taaacggcgc ttcggtacag tagttcacca
    27301 cttcggtctt tgctccgagc aatttctcaa ctacttcaac cagttgatga ttcggtgggc
    27361 attcatagcc agggatcggc ggatgcagct cgtcgaccgt cagacgaccc ggccagcgtt
    27421 cgctcaccgg agccaatgca tcgttgagca aaccattaag ttcattgagt gtcatgccag
    27481 gcagcggacg aatatccata tgcaactcac agcaagcgca aatacggtta gaagcgtcgc
    27541 caccgtgaat atgcccgagg ttgagcgtag ggtatggcac ggtaaacgct tcgtagtgat
    27601 aacgttcttt caggttatcg cgcaattgca aaatatgccc gatggcgtcg tgcattagtt
    27661 cgatagcgtt aactccgcgt gctggatcgc tggagtgccc cgactggccc tgaatacgga
    27721 tggcgttaga gatatgacct ttatgtgcgc gtaccggttg tagtgacgtc ggttcgccaa
    27781 tgatggcgca atccgggcgc agggcggtag tttcggcaaa ataacgcgct ccggccatac
    27841 tggtttcttc atcagcagtc gccagaatgt agagcggttt tttcagtttc gtgacgtcga
    27901 catcgcgtag cgcatcaagg ataaacgcaa aaaagccttt catgtcggcg gtgcctaagc
    27961 cgtaaagctt gccgtcatgc tccgtcagtg taaacggatc gcgcgtccag cgaccgtcat
    28021 caaatggcac cgtatcggta tgccccgcca gcaacaagcc gccagccccc tgtccgatac
    28081 ttgccagcat attgaatttg ttgcgagttc ctggcacagg ctgcacttcc acattgaagc
    28141 ccaaatcttt aaaccagtcc gccagcagag tgattaaatc tgcattgctt tgatcgagtg
    28201 cctcttccgt ggcgcttatt gaaggtgtgg caatcagagc acggtaaatc tcgataaatg
    28261 gcggtaattt gtttttcatt gttgacacac ctctggtcat gatagtatca atattcatgc
    28321 agtatttatg aataaaaata cactaacgtt gagcgtaata aaacccacca gccgtaaggt
    28381 gaatgtttta cgtttaacct ggcaaccaga cataagaagg tgaatagccc cgatgttgaa
    28441 tacgctgatt gtgggtgcca gcggctacgc tggcgcagag ctagtgacct atgtaaatcg
    28501 ccatccgcat atgaacataa ccgctttgac tgtttcagcg caaagcaatg atgcgggaaa
    28561 gttaatctcc gatttgcatc cgcagctaaa aggcatcgtt gatctgccgt tgcagccgat
    28621 gtcggatatc agcgagttta gcccaggggt ggacgtagtg tttctcgcca ccgcccatga
    28681 agttagccac gatttagcgc cgcagtttct tgaagcgggc tgcgtggtgt tcgacctttc
    28741 cggcgcgttt cgtgttaacg acgccacctt ctatgaaaaa tattacggct ttacccatca
    28801 atacccggaa ctgctggaac aggcagccta cggtctggcg gagtggtgcg gtaataaatt
    28861 aaaagaagcg aatttgattg cggtgccagg ctgttatccg acggcggcac agctggcgct
    28921 gaaaccgttg attgatgccg atcttcttga cctcaatcag tggccggtga tcaacgccac
    28981 cagcggcgtg agcggtgcag gacgtaaggc agcgatttca aatagttttt gtgaagttag
    29041 cctgcaaccg tatggcgtct ttactcatcg ccatcaacca gagatcgcca cacacctcgg
    29101 tgctgacgtt atcttcactc cgcatctggg caatttcccg cgcggcattc tcgaaaccat
    29161 tacctgccgc ctgaaatcgg gtgtgaccca ggcgcaagtc gcgcaagtgt tacagcaggc
    29221 gtatgcccat aaaccgctgg tgcggctgta tgacaaaggc gttccggcgc tgaaaaatgt
    29281 cgttgggctg ccattttgcg atatcgggtt tgccgttcag ggcgagcatc tgattattgt
    29341 ggcgaccgaa gacaacttac tgaaaggcgc ggcggcacaa gcggtacagt gcgccaatat
    29401 tcgtttcggc tatgcggaaa cgcagtctct tatttaaggg tgcaatgatg aatccattaa
    29461 ttatcaaact gggcggcgta ctgctggata gtgaagaggc gctggaacgt ctgtttagcg
    29521 cactggtgaa ttatcgtgag tcacatcagc gtccgctggt gattgtgcac ggcggcggtt
    29581 gcgtggtgga tgagctgatg aaagggctga atctgccggt gaaaaagaaa aacggcctgc
    29641 gggtgacgcc tgctgatcag atagacatta tcaccggagc actggcggga acggcaaata
    29701 aaaccctgtt ggcatgggcg aagaaacatc agattgcggc cgtaggtttg tttctcggtg
    29761 acggcgacag cgtcaaagtg acccagcttg atgaagagtt aggtcatgtt ggactggcgc
    29821 agccaggttc gcctaagctt atcaactcct tgctggagaa cggttatctg ccggtggtca
    29881 gctccattgg cgtaacagac gaagggcaac tgatgaacgt caatgccgac caggcggcaa
    29941 cggcgctggc ggcaacgctg ggcgcggatc tgattttgct ctccgacgtc agcggcattc
    30001 tcgacggcaa agggcaacgc attgccgaaa tgaccgccgc gaaagcagaa caactgattg
    30061 agcagggcat tattactgac ggcatgatag tgaaagtgaa cgcggcgctg gatgcggccc
    30121 gcacgctggg ccgtccggta gatatcgcct cctggcgtca tgcggagcag cttccggcac
    30181 tgtttaacgg tatgccgatg ggtacgcgga ttttagctta agttttgttg gccggaggcg
    30241 cagctttccg gcattgaatt tcaaaataag gaaacagagt tatggcactt tggggcgggc
    30301 gttttaccca ggcagcagat caacggttca aacaattcaa cgactcactg cgctttgatt
    30361 accgtctggc ggagcaggat attgttggct ctgtggcctg gtccaaagcc ctggtcacgg
    30421 taggcgtgtt aaccgcagaa gagcaggcgc aactggaaga ggcgctgaac gtgctgttgg
    30481 aagatgttcg cgccaggcca caacaaatcc ttgaaagcga cgccgaagat atccatagct
    30541 gggtggaagg caaactgatc gacaaagtgg gccagttagg caaaaagctg cataccgggc
    30601 gtagtcgtaa cgatcaggta gcgactgacc tgaaactgtg gtgcaaagat accgttagcg
    30661 agttactgac ggctaaccga cagctgcaat cggcgctggt ggaaaccgca caaaacaatc
    30721 aggacgcggt catgccaggt tacactcacc tgcaacgcgc ccagccggtg acgttcgcgc
    30781 actggtgcct ggcctatgtt gagatgctgg cgcgtgatga aagccgtttg caggatgcgc
    30841 ttaagcgtct ggatgtcagc ccattaggct gtggcgcgct ggcgggaacg gcctatgaaa
    30901 tcgaccgtga acagttagca ggctggctgg gctttgcttc ggcgacccgt aacagtctgg
    30961 acagcgtttc tgaccgtgac catgtgttgg aactgctgtc ggctgccgct atcggcatgg
    31021 tgcatctgtc gcgttttgct gaagatctga ttttctttaa caccggcgaa gcggggtttg
    31081 tggagctttc tgaccgcgtg acttccggtt catcattaat gccgcagaag aaaaacccgg
    31141 atgcgctgga gctgattcgc ggtaaatgcg gtcgggtgca gggggcgtta accggcatga
    31201 tgatgacgct gaaaggtttg ccgctggctt acaacaaaga tatgcaggaa gacaaagaag
    31261 gtctgttcga cgcgctcgat acctggctgg actgcctgca tatggcggcg ctggtgctgg
    31321 acggcattca ggtgaaacgt ccacgttgcc aagaagcggc tcagcagggt tacgccaacg
    31381 ccaccgaact ggcggattat ctggtggcga aaggcgtacc gttccgcgag gcgcaccata
    31441 ttgttggtga agcggtggtg gaagccattc gtcagggcaa accgctggaa gatctgcctc
    31501 tcagtgagtt gcagaaattc agccacgtga ttggcgaaga tgtctatccg attctgtcgc
    31561 tacaatcgtg cctcgacaag cgtgcggcaa aaggcggcgt ctcaccgcag caggtggcgc
    31621 aggcgattgc ttttgcgcag gctcggttag agtaatttgt ttatattact cccctgaaaa
    31681 tggagagctt accacgccta caaagtgcga cacttcgtag gcggataagg cgtttacgcc
    31741 acatcaggca gaaatttgtc agcattctgg tgaccttaaa tataacgccc aaccagaacc
    31801 attatttgtc acattccttt ttatcattgt gtcttcttta ccttcacact ccccatgaaa
    31861 atcatatttt caaatacatc aaccaataca tattttaatt aattgaatat aattattttc
    31921 tattgctgtg cccattttca atgattaatg gagtttgtta tgtgattatt taacgtgttg
    31981 ttttaacgat aatggttaat tattcaggtg gtttttgagg gtattttttc tcctcggtgg
    32041 aaatttttgt ttttaatgtg atcacggttg aataatctct ttttctctct tgtatggtag
    32101 tatgcgtaaa attaagtttg aaacgtctat ttacaacaca ggagattgca ataatgaaaa
    32161 ttattgctgc agatgttttt gttacttgcc cagggcgtaa tttcgttacg cttaaaataa
    32221 ccactgaaag cggactttgt ggattaggag atgccacgtt aaatggtcgc gaattgtcgg
    32281 tcgcttctta tctgaaagat catctttgtc cacaactaat tggccgcgat gccagccgta
    32341 tcgaagatat ctggcaattc ttttataaag gtgcttactg gcgacgtggc cccgtcacga
    32401 tgtcagccat ttctgccatt gatatggcgt tgtgggatat caaagctaaa gcggcaaata
    32461 tgccattgta tcaactgctt ggtggtgctt ctcgcgaagg cgtgatggtt tattgccaca
    32521 ccaccgggcg gacgattgat gaagtccttg aagactatgc aaagcaccag cagatgggct
    32581 ttaaggcgat tcgtgtgcag tgcggtgtac cgggtatgca aactacttat ggcctggcga
    32641 agggcaaagg cctggcgtat gaaccggcaa ccaaaggcct gtggccagaa gagcagttgt
    32701 ggtcttcaga aaagtatctc gattttacgc cgaagttatt tgaagcggtg cgtaataagt
    32761 tcggttttaa cgaacatctg ttgcatgaca tgcaccatcg cctgaccccc atcgaagcgg
    32821 cgcgctttgg gaagagcatt gaggattacc gcctgttctg gatggaagat ccgacgcctg
    32881 cagagaacca ggaatgtttt cgtctgattc gccagcatac cgtgacgccg attgcggtcg
    32941 gtgaagtgtt taacagtatc tgggactgca agcagttaat cgaagagcag ctgattgact
    33001 atattcgcac gacgatcacc catgcaggcg gcattaccgg gatgcggcgg attgccgatt
    33061 ttgcatcgct ttatcaggta cgtaccggtt cgcatggccc ttcagattta tcgcctgttt
    33121 gtcatcggcg gcgctgcatt ttgacctatg ggtaccaaat tttggcgttc aggagtacat
    33181 gggctattcc gaacaaatgc ttgaagtttt cccgcacagc tggcgcttcg aggaaggtta
    33241 tatgcaccca ggcgatgaac cggggctggg tatttctttc gatgagaaac tggcggcgaa
    33301 atatccgtat gacccggcgt atctccccgt cgcacgactg gaagatggca cattgtggaa
    33361 ctggtaataa cggttcgctt aattattttc tgaatagtca tcaaatcgat taattccgct
    33421 caaggagcta aaaatggacg ttgatgtgtc aaccagtgtt gcgggtaata aaccgcaacg
    33481 gattcgtcgt attcagacag tgaccctggt tttattattt atggcgggga tcgttaattt
    33541 tctcgaccgt tcgtcattga gcgtggcagg tgaagcgatt cgtggcgagt taggattatc
    33601 ggcgacagag tttggtgttt tgctttctgc attttctctg tcttatggtt tttcacaact
    33661 accttccggt attttgctag accgctttgg cccacgaatt gttttaggcg caggcttaat
    33721 attctggtca ttaatgcagg cattaacagg aatggttaat tcttttagcc actttatttt
    33781 aatgcgtatc ggtctgggta ttggcgaagc gccatttatg cctgcgggag taaagtcgat
    33841 caccgactgg tatgcacaaa aagaacgcgg cacggcgctg gggattttta actcatctac
    33901 cgttatcggt caggctatcg cgcctcctgc tcttgtattg atgcagctgg catggggctg
    33961 gcggacgatg ttcgttatca tcggcgtggc agggattctg gttgggatct gttggtacgc
    34021 gtggtatcgc aaccgggcgc agtttgtcct gactgacgaa gaacggacgt atctctccgc
    34081 gccggttaaa ccgcgtccac agctgcaatt tagcgagtgg ctggcgctgt ttaagcatcg
    34141 gacaacctgg gggatgattt taggtttctc tggcgtcaat tataccggtt ggctctacat
    34201 cgcgtggtta cccggttatt tgcaagccga gcaaggtttc agtctggcga aaaccggttg
    34261 ggtggcggcg attcctttcc tcgcggcggc agtcgggatg tgggttaacg gtattgttgt
    34321 cgatcgactg gcgaaaaaag gctacgacct ggcgaagacg cgtaaaacgg ctattgtctg
    34381 cggtttgatg atgtcggcat taggcacgtt gctggtcgtg caatcttcct cgccagccca
    34441 ggcggtagcg tttatctcaa tggcgctatt ctgcgtgcat ttcgctggaa cgtctgcatg
    34501 ggggctggtg caggtgatgg tgtcagaaac aaaagtggct tccatcgccg ggattcaaaa
    34561 ctttggcagt tttgtttttg cttcctttgc tccgatcgta accggttggg tagtggatac
    34621 cacacactcg tttaatctgg cgttggttat cgcggcctgt gtgacgttca ccggggcgct
    34681 gtgttacttc tttattgtca aagatcgcat tgagtaatcg ttactatcag gctctcttgc
    34741 tgtgggcctg tagaataaaa aaaagcggat cctggagatc cgcaaaagtt cacgttggct
    34801 ttagttattc gagttgagaa actctcgaaa cgggcagtga cttcaagggt taaaagaggt
    34861 gccgctccgt tcctgtgagc aattatcagt cagaatgctt gatagggata atcgttcatt
    34921 gctattcttc ctatcgccat gaactatcgt ggcgatggag gatggataat gaatattcgt
    34981 gatcttgagt acctggtggc attggctgaa caccgccatt ttcggcgtgc ggcagactcc
    35041 tgccacgtta gccagccgac gcttagcggg caaattcgta agctggaaga tgagctgggc
    35101 gtgatgttgc tggagcggac cagccgtaaa gtgttgttca cccaggcggg aatgctgctg
    35161 gtggatcagg cgcgtaccgt gctgcgtgag gtgaaagtcc ttaaagagat ggcaagccag
    35221 cagggtgaga ccatgtccgg gccgctgcat attggtttga ttcccacggt cgggccgtac
    35281 ctgctaccgc atattatccc gatgctgcac cagacctttc caaagctgga aatgtatctg
    35341 catgaagcac agacccacca gttactggcg caactggaca gcggcaaact cgattgcgtg
    35401 atcctcgcgc tggtgaaaga gagcgaagca ttcattgaag tgccgttgtt tgatgagcca
    35461 atgttgctgg ctatctatga agatcacccg tgggcgaacc gcgaatgcgt accgatggcc
    35521 gatctggcag gggaaaaact gctgatgctg gaagatggtc actgtttgcg cgatcaggca
    35581 atgggtttct gttttgaagc cggggcggat gaagatacac acttccgcgc gaccagcctg
    35641 gagacgctgc gcaacatggt ggcggcaggt agcgggatca ctttactgcc tgcgctggct
    35701 gtgccgccgg agcgcaaacg cgatggggtt gtttatctgc cgtgcattaa gccggaacca
    35761 cgccgcacta ttggcctggt ttatcgtcct ggctcaccgc tgcgcagccg ctatgagcag
    35821 ctggcagagg ccatccgcgc acgaatggat ggccatttcg ataaagtgtt aaaacaggcg
    35881 gtttaaaccg tttaacgcag ctacccgata ggcttccgcc atggtcgggt agttaaaggt
    35941 ggtgttgacg aagtactcaa tagtgttgcc gccacctttc tgttccataa tcgcctgacc
    36001 gatatgaata atttcggcag cgcgctcgcc aaagcagtga atacccagaa tctcttttgt
    36061 ttcccgatgg aacaaaattt tcagcgtgcc cacgttcatg ccgacgattt gtgcgcgtgc
    36121 cagatgttta aactgggcgc ggcccacttc atatggcact ttcatcgcgg tcagctgctg
    36181 ttcggttttg cccacagagc tgatttccgg gatggtgtaa atgccggtag ggatatcttc
    36241 aatcagatgc gcggtggctt cgccttttac cagcgcctgc gcggcaatgc gcccctggtc
    36301 ataggccgcc gacgccaggc tcggataacc aatcacgtcg cccaccgcgt aaacgtgcgg
    36361 ctgtgcggtc tgatacatgc tgttgacctt cagctgtccg cgactgtcag tttccagccc
    36421 aatgttctgt aacgccagcg aatcagtatt accggtgcga ccgttggcat agagcaggca
    36481 gtcagctttc agttttttac ccgacttcag atgcatgatc acaccgtcgt cacagccttc
    36541 gatcttctcg tactcttcgt tgtggcgaat cactacgcca ctgttccaga agtgatagga
    36601 gagagaatct gacatctctt gatcgagaaa tgccagcagc cgatcgcggg tgttgatcag
    36661 atccactttt acatccatac cgcggaagat cgacgcatat tcacagccga tcactccagc
    36721 accatagata agtacatggc gcggttcgtg gtgcatgcta agaattgagt cgctgtcgta
    36781 aatgcgtgga tgggtgaaat caacatctgt tggatgatat ggacgagagc cgcaggcaat
    36841 aacaaatttt tcagcggtta gtgtttcaac gctgccgtcc gggcaatcca gcgccaacgt
    36901 atgctcgtca acaaagcgag cgtttccctg caatatttca cagtgattac gttcgtaaaa
    36961 tccctgacgc atgcgcgttt gttgattaat cacgttatcg gcatggttaa ggatatcggc
    37021 aaaagaagag cggagcagtc gggaatggtc gctgtaaagt gggttttgat tgaattctat
    37081 aatgcggctg acggcgtgac ggagagcttt cgacgggatg gtgccccagt gggtgcaacc
    37141 gccgccaaca ttttgataac gctcgataac tgcgacgcgc gcaccttgct taaccaggcc
    37201 cattgcagcg ccttcgccgc cggggccgga acctattact atggcatcgt aatcgtagga
    37261 atgtggcatg gtagggctta cctgttctta tacataaaag caacagaatg gtaacatttt
    37321 atcgcgggta agccaattga tccccgtcat ttatctggct atatcctgag cggcctttgc
    37381 tttgtctgtt tcttactttt gccctgacgt tttattggat ttttatcgac gatactctcc
    37441 gtttaagcgg caggtttccg ctgtacgtaa aagaaccggc cagtaaatat gttttattgc
    37501 gttaccgttc attcacaata ctggagcaat ccagtatgtt cattctctgg tatagtgcca
    37561 gcagtacttt tggcaaggat tcagacatcg tgatgggcat aagagcgcaa caaaaagaaa
    37621 aaacccgccg ttcgctggtg gaagccgcat ttagccaatt aagtgctgaa cgcagcttcg
    37681 ccagcctgag tttgcgtgaa gtggcgcgtg aagcgggcat tgctcccacc tctttttatc
    37741 ggcatttccg cgacgtagac gaactgggtc tgaccatggt tgatgagagc ggtttaatgc
    37801 tacgccaact catgcgccag gcgcgtcagc gtatcgccaa aggcgggagt gtgatccgca
    37861 cctcggtctc cacatttatg gagttcatcg gtaataatcc taacgccttc cggttattat
    37921 tgcgggaacg ctccggcacc tccgctgcgt ttcgtgccgc cgttgcgcgt gaaattcagc
    37981 acttcattgc ggaacttgcg gactatctgg aactcgaaaa ccatatgccg cgtgcgttta
    38041 ctgaagcgca agccgaagca atggtgacaa ttgtcttcag tgcgggtgcc gaggcgttgg
    38101 acgtcggcgt cgaacaacgt cggcaattag aagagcgact ggtactgcaa ctgcgaatga
    38161 tttcgaaagg ggcttattac tggtatcgcc gtgaacaaga gaaaaccgca attattccgg
    38221 gaaatgtgaa ggacgagtaa tgaaacaagc aaatcaagat agaggtacgc tgctgctggc
    38281 gttagttgct ggcttatcga ttaatggtac tttcgcagcg ctgtttagct ccattgtgcc
    38341 attttctgta ttcccgatta tttccctggt gctgacggtt tactgcctgc atcaacgtta
    38401 tcttaatcgc actatgccgg taggcttgcc gggtctggca gctgcctgtt ttattctcgg
    38461 cgtactgctg tacagcacgg tagttcgtgc ggaatatccg gatatcggct ctaacttctt
    38521 cccggcagta ctctccgtca ttatggtgtt ctggattggc gcgaagatgc gtaaccgtaa
    38581 gcaggaagtt gctgagtaat cggtattatg ccggatgctg gcgcatccgg catgggtttt
    38641 acttcgcggt cagtaatacg ccgcactcca tatggtgcgt gtaggggaac tgatcaaaca
    38701 gagccagacg ttcgaccttg tgcgtctggc ttaatgtttc cagattcttg cataacgttt
    38761 ccgggttaca ggagatgtac aaaatacgcg gatacgcctg caccattttc tcggtttcac
    38821 tgtccagacc gctgcgcggt gggtcgacaa aaatggtttc gcactgataa ctctttaagt
    38881 cgatcccttg caggcggtta aactcgcgca caccattcat cgcctgagta aattcttctg
    38941 ccgccatacg aataatttgt acgttatcaa tatggttagc tgcgatgttg tattgcgcag
    39001 cagcaaccga cggcttagcg atttcggtgg ctaatacccg atcaaaattg cgcgccagcg
    39061 ctaatgaaaa gttaccgttg ccgcagtaca gctccagtaa atcgcctttt gagcctttgg
    39121 ttacgtccag cgcccattcc agcatctgaa tattcatcgc cgcgttcggc tgggtaaaac
    39181 tgttttctac ctgacggtag atcatctctt tccctgcgac cggcagacgt tcatcgatgt
    39241 aatcttgatc cagctcgatt ttggttttcg ttgcccgacc aatcagatgc acattcagat
    39301 tctgcgcgcg cagtgcatcg cgcagggcct ccgcttcctg acgccactca tcatccagct
    39361 tcttatggta tagcagggaa accaccgcct gattactcag cgtggtgagg taatcaatct
    39421 ggaacaactt gtggcgcaga acgggattat tacgcacacc cgcaatcatc gccgtcatca
    39481 actggttgat gagttcactg gcagcgggga agctatccac gcggatgcgg cttttggttt
    39541 gttgatcgaa aatgatgtga tacaggtcat cgccatcgtg ccagatgcgg aactccgcac
    39601 gcatccggta atgactgacc ggcgagcgaa acacttccgg aaccaggtca gaaaacggtg
    39661 ccatcatact ttgcaaacgt accacttttt cggctaactg cgcttcatac tgttctgttg
    39721 gaaggtgttc gggggtcatg atgtatcctg aaaaattaaa gtacgcggcg attgtaggga
    39781 ttgctcatca gatgtccaga tcttgatgaa ttcctatttg tgagctacgt ctggacagta
    39841 acttgttaca acctgtagca tccacttgcc ggtcctgtga gttaataggg aatccagtgc
    39901 gaatctggag ctgacgcgca gcggtaagga aaggtgcgat gattgcgtta tgcggacact
    39961 gccatccggt gggaagtcat catctcttag tatcttagat acccctccaa gcccgaagac
    40021 ctgccggcca acgtcgcatc tggttctcat catcgcgtaa tattgatgaa acctgcggca
    40081 tccttcttct attgtggatg ctttacaatg attaaaaaag cttcgctgct gacggcgtgt
    40141 tctgtcacag ccttttccgc ttgggcacag gataccagcc cggatactct cgtcgttact
    40201 gctaaccgtt ttgaacagcc gcgcagcact gtgcttgcac caaccaccgt tgtgacccgt
    40261 caggatatcg accgctggca gtcgacctcg gtcaatgatg tgctgcgccg tcttccgggc
    40321 gtcgatatca cccaaaacgg cggttcaggt cagctctcat ctatttttat tcgcggtaca
    40381 aatgccagtc atgtgttggt gttaattgat ggcgtacgcc tgaatctggc gggggtgagt
    40441 ggttctgccg accttagcca gttccctatt gcgcttgtcc agcgtgttga atatatccgt
    40501 gggccacgct ccgccgttta tggttccgat gcaataggcg gggtggtgaa tatcatcacg
    40561 acgcgcgatg aacccggaac ggaaatttca gcagggtggg gaagcaatag ttatcagaac
    40621 tatgatgtct ctacgcagca acaactgggg gataagacac gggtaacgct gttgggcgat
    40681 tatgcccata ctcatggtta tgatgttgtt gcctatggta ataccggaac gcaagcgcag
    40741 acagataacg atggtttttt aagtaaaacg ctttatggcg cgctggagca taactttact
    40801 gatgcctgga gcggctttgt gcgcggctat ggctatgata accgtaccaa ttatgacgcg
    40861 tattattctc ccggttcacc gttgctcgat acccgtaaac tctatagcca aagttgggac
    40921 gccgggctgc gctataacgg cgaactgatt aaatcacaac tcattaccag ctatagccat
    40981 agcaaagatt acaactacga tccccattat ggtcgttatg attcgtcggc gacgctcgat
    41041 gagatgaagc aatacaccgt ccagtgggca aacaatgtca tcgttggtca cggtagtatt
    41101 ggtgcgggtg tcgactggca gaaacagact acgacgccgg gtacaggtta tgttgagaat
    41161 ggatatgatc aacgtaatac cggcatctat ctgaccgggc tgcaacaagt cggcgatttt
    41221 acctttgaag gcgcagcacg cagtgacgat aactcacagt ttggtcgtca tggaacctgg
    41281 caaaccagcg ccggttggga attcatcgaa ggttatcgct tcattgcttc ctacgggaca
    41341 tcttataagg caccaaatct ggggcaactg tatggcttct acggaaatcc gaatctggac
    41401 ccggagaaaa gcaaacagtg ggaaggcgcg tttgaaggct taaccgctgg ggtgaactgg
    41461 cgtatttccg gatatcgtaa cgatgtcagt gacttgatcg attatgatga tcacaccctg
    41521 aaatattaca acgaagggaa agcgcggatt aagggcgtcg aggcgaccgc caattttgat
    41581 accggaccac tgacgcatac tgtgagttat gattatgtcg atgcgcgcaa tgcgattacc
    41641 gacacgccgt tgttacgccg tgctaaacag caggtgaaat accagctcga ctggcagttg
    41701 tatgacttcg actggggtat tacttatcag tatttaggca ctcgctatga taaggattac
    41761 tcatcttatc cttatcaaac cgttaaaatg ggcggtgtga gcttgtggga tcttgcggtt
    41821 gcgtatccgg tcacctctca cctgacagtt cgtggtaaaa tagccaacct gttcgacaaa
    41881 gattatgaga cagtctatgg ctaccaaact gcaggacggg aatacacctt gtctggcagc
    41941 tacaccttct gaaccacgtc ccaccgtgct ggtgtttgac tccggcgtcg gtgggttgtc
    42001 ggtctatgac gagatccggc atctcttacc ggatctccat tacatttatg ctttcgataa
    42061 cgtcgctttt ccgtatggcg aaaaaagcga agagtttatt gttgagcgag tggtggcaat
    42121 tgtcaccgca gtgcaagaac gttatcccct tgcgctggct gtggtcgctt gcaacaccgc
    42181 cagtaccgtt tcacttcctg cattacgcga aaagttcgac ttcccggttg ttggtgtcgt
    42241 gccggcgatt aaacctgctg cacgtctgac ggcaaatggc attgtcggat tactggcaac
    42301 ccgcggaact gttaaacgtt cttatactca tgagctgatc gcgcgtttcg ctaatgaatg
    42361 ccagatagaa atgctgggct cggcagagat ggttgagttg gctgaagcga agctgcatgg
    42421 cgaagatgtt tctctggatg cactaaaacg tatcctacgc ccgtggttaa gaatgaaaga
    42481 gccgccagat accgttgtat tgggttgcac ccatttccct ctactacaag aagaactctt
    42541 acaggtgctg ccagagggaa cccggctggt ggattctggc gcagcgattg ctcgcagaac
    42601 ggcctggctg ttagaacatg aagctccgga tgcaaaatct gccgatgcga atattgcctt
    42661 ttgtatggca atgacgccag aagctgaaca attattgccc gttttacagc gttacggctt
    42721 cgaaacgctc gaaaaactgg cagttttagg ctgatttggt tgaatgttgc gcggtcggaa
    42781 aattatttta aatttcctct tgtcaggccg gaataactcc ctataatgcg ccaccactga
    42841 cacggaacaa tggcaaacac gccgccgggt cagcggggtt ctcctgagaa ttccgacaga
    42901 gaaagcaaaa ataaatgctt gactctgtag c
//
LOCUS       DAZFQD010000024        42926 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.024, whole genome shotgun sequence.
ACCESSION   DAZFQD010000024 DAZFQD010000000
VERSION     DAZFQD010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 42926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42926
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.024"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(68..502)
                     /gene="merR"
                     /locus_tag="ACHKTW_001394"
     CDS             complement(68..502)
                     /gene="merR"
                     /locus_tag="ACHKTW_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0045340 - mercury ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0046689 - response to mercury ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hg(II)-responsive transcriptional regulator"
                     /protein_id="HFO6462450.1"
                     /translation="MENNLENLTIGVFAKAAGVNVETIRFYQRKGLLREPDKPYGSIR
                     RYGEADVVRVKFVKSAQRLGFSLDEIAELLRLDDGTHCEEASSLAEHKLKDVREKMAD
                     LARMETVLSELVCACHARKGNVSCPLIASLQGEAGLARSAMP"
     gene            574..924
                     /locus_tag="ACHKTW_001395"
     CDS             574..924
                     /locus_tag="ACHKTW_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mercuric transport protein MerT"
                     /protein_id="HFO6462451.1"
                     /translation="MSEPQNGRGALFAGGLAAILASTCCLGPLVLVALGFSGAWIGNL
                     TVLEPYRPLFIGAALVALFFAWKRIYRPVQACKPGEVCAIPQVRATYKLIFWIVAVLV
                     LVALGFPYVVPFFY"
     gene            938..1213
                     /gene="merP"
                     /locus_tag="ACHKTW_001396"
     CDS             938..1213
                     /gene="merP"
                     /locus_tag="ACHKTW_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004636750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0045340 - mercury ion binding [Evidence
                     IEA];
                     GO_process: GO:0046689 - response to mercury ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mercury resistance system periplasmic binding
                     protein MerP"
                     /protein_id="HFO6462452.1"
                     /translation="MKKLFASLALAAAVAPVWAATQTVTLAVPGMTCAACPITVKKAL
                     SKVEGVSKVDVGFEKREAVVTFDDTKASVQKLTKATADAGYPSSVKQ"
     gene            1249..1671
                     /gene="merC"
                     /locus_tag="ACHKTW_001397"
     CDS             1249..1671
                     /gene="merC"
                     /locus_tag="ACHKTW_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016239395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organomercurial transporter MerC"
                     /protein_id="HFO6462453.1"
                     /translation="MGLMTRIADKTGALGSVVSAMGCAACFPALASFGAAIGLGFLSQ
                     YEGLFISRLLPLFAALAFLANALGWFSHRQWLRSLLGMIGPAIVFAATVWLLGNWWTA
                     NLMYVGLALMIGVSIWDFVSPAHRRCGPDGCELPAKRL"
     gene            1723..3417
                     /gene="merA"
                     /locus_tag="ACHKTW_001398"
     CDS             1723..3417
                     /gene="merA"
                     /locus_tag="ACHKTW_001398"
                     /EC_number="1.16.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016152 - mercury (II) reductase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0045340 - mercury ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0046689 - response to mercury ion [Evidence
                     IEA];
                     GO_process: GO:0050787 - detoxification of mercury ion
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mercury(II) reductase"
                     /protein_id="HFO6462454.1"
                     /translation="MSTLKITGMTCDSCAVHVKDALEKVPGVQSADVSYAKGSAKLAI
                     EVGTSPDALTAAVAGLGYRATLADAPSVSTPGGLLDKMRDLLGRNDKTGSSGALHIAV
                     IGSGGAAMAAALKAVEQGARVTLIERGTIGGTCVNVGCVPSKIMIRAAHIAHLRRESP
                     FDGGIAATTPTIQRTALLAQQQARVDELRHAKYEGILEGNPAITVLHGSARFKDNRNL
                     IVQLNDGGERVVAFDRCLIATGASPAVPPIPGLKDTPYWTSTEALVSETIPKRLAVIG
                     SSVVALELAQAFARLGAKVTILARSTLFFREDPAIGEAVTAAFRMEGIEVREHTQASQ
                     VAYINGEGDGEFVLTTAHGELRADKLLVATGRAPNTRKLALDATGVTLTPQGAIVIDP
                     GMRTSVEHIYAAGDCTDQPQFVYVAAAAGTRAAINMTGGDAALNLTAMPAVVFTDPQV
                     ATVGYSEAEAHHDGIKTDSRTLTLDNVPRALANFDTRGFIKLVVEEGSGRLIGVQAVA
                     PEAGELIQTAALAIRNRMTVQELADQLFPYLTMVEGLKLAAQTFNKDVKQLSCCAG"
     gene            3435..3797
                     /gene="merD"
                     /locus_tag="ACHKTW_001399"
     CDS             3435..3797
                     /gene="merD"
                     /locus_tag="ACHKTW_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0046689 - response to mercury ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mercury resistance co-regulator MerD"
                     /protein_id="HFO6462455.1"
                     /translation="MSAYTVSQLAHNAGVSVHIVRDYLVRGLLRPVACTTGGYGVFDD
                     AALQRLCFVRAAFEAGIGLDALARLCRALDAADGAQAAAQLAVLRQLVERRRAALAHL
                     DAQLASMPAERAHEEALP"
     gene            3794..4030
                     /gene="merE"
                     /locus_tag="ACHKTW_001400"
     CDS             3794..4030
                     /gene="merE"
                     /locus_tag="ACHKTW_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004102820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="broad-spectrum mercury transporter MerE"
                     /protein_id="HFO6462456.1"
                     /translation="MNAPDKLPPETRQPVSGYLWGALAVLTCPCHLPILAAVLAGTTA
                     GAFLGEHWGVAALALTGLFVLAVTRLLRAFRGGS"
     gene            4027..4674
                     /locus_tag="ACHKTW_001401"
                     /pseudo
     CDS             4027..4674
                     /locus_tag="ACHKTW_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003132006.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            4809..6488
                     /locus_tag="ACHKTW_001402"
     CDS             4809..6488
                     /locus_tag="ACHKTW_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013124600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu transposase C-terminal domain-containing
                     protein"
                     /protein_id="HFO6462457.1"
                     /translation="MATDTPRIPEQGVATLPDEAWERARRRAEIISPLAQSETVGHEA
                     ADMAAQALGLSRRQVYVLIRRARQGSGLVTDLVPGQSGGGKGKGRLPEPVERVIHELL
                     QKRFLTKQKRSLAAFHREVTQVCKAQKLRVPARNTVALRIASLDPRKVIRRREGQDAA
                     RDLQGVGGEPPAVTAPLEQVQIDHTVIDLIVVDDRDRQPIGRPYLTLAIDVFTRCVLG
                     MVVTLEAPSAVSVGLCLVHVACDKRPWLEGLNVEMDWQMSGKPLLLYLDNAAEFKSEA
                     LRRGCEQHGIRLDYRPLGQPHYGGIVERIIGTAMQMIHDELPGTTFSNPDQRGDYDSE
                     NKAALTLRELERWLTLAVGTYHGSVHNGLLQPPAARWAEAVARVGVPAVVTRATSFLV
                     DFLPILRRTLTRTGFVIDHIHYYADALKPWIARRERWPSFLIRRDPRDISRIWVLEPE
                     GQHYLEIPYRTLSHPAVTLWEQRQALAKLRQQGREQVDESALFRMIGQMREIVTSAQK
                     ATRKARRDADRRQHLKTSARPDKPVPPDTDIADPQADNLPPAKPFDQIEEW"
     gene            6491..7351
                     /locus_tag="ACHKTW_001403"
     CDS             6491..7351
                     /locus_tag="ACHKTW_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TniB family NTP-binding protein"
                     /protein_id="HFO6462458.1"
                     /translation="MDEYPIIDLSHLLPAAQGLARLPADERIQRLRADRWIGYPRAVE
                     ALNRLEALYAWPNKQRMPNLLLVGPTNNGKSMIVEKFRRTHPASSDADQEHIPVLVVQ
                     MPSEPSVIRFYVALLAAMGAPLRPRPRLPEMEQLALALLRKVGVRMLVIDELHNVLAG
                     NSVNRREFLNLLRFLGNELRIPLVGVGTRDAYLAIRSDDQLENRFEPMMLPVWEANDD
                     CCSLLASFAASLPLRRPSPIATLDMARYLLTRSEGTIGELAHLLMAAAIVAVESGEEA
                     INHRTLSMAC"
     gene            7453..8976
                     /gene="istA"
                     /locus_tag="ACHKTW_001404"
     CDS             7453..8976
                     /gene="istA"
                     /locus_tag="ACHKTW_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001324342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS1326 family transposase"
                     /protein_id="HFO6462459.1"
                     /translation="MINVAILSAIRRWHFRDGASIREIARRSGLSRNTVRKYLQSKVV
                     EPQYPARDSVGKLSPFEPKLRQWLSTEHKKTKKLRRNLRSMYRDLVALGFTGSYDRVC
                     AFARQWKDSEQFKAQTSGKGCFIPLRFACGEAFQFDWSEDFARIAGKQVKLQIAQFKL
                     AHSRAFVLRAYYQQKHEMLFDAHWHAFQIFGGIPKRGIYDNMKTAVDSVGRGKERRVN
                     QRFTAMVSHYLFDAQFCNPASGWEKGQIEKNVQDSRQRLWQGAPDFQSLADLNVWLEH
                     RCKALWSELRHPELDQTVQEAFADEQGELMALPNAFDAFVEQTKRVTSTCLVHHEGNR
                     YSVPASYANRAISLRIYADKLVMAAEGQHIAEHPRLFGSGHARRGHTQYDWHHYLSVL
                     QKKPGALRNGAPFAELPPAFKKLQSILLQRPGGDRDMVEILALVLHHDEGAVLSAVEL
                     ALECGKPSKEHVLNLLGRLTEEPPPKPIPIPKGLRLTLEPQANVNRYDSLRRAHDAA"
     gene            8963..9748
                     /gene="istB"
                     /locus_tag="ACHKTW_001405"
     CDS             8963..9748
                     /gene="istB"
                     /locus_tag="ACHKTW_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000983249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS1326 family helper ATPase
                     IstB"
                     /protein_id="HFO6462460.1"
                     /translation="MMQHEGHVRILKSLKLFGMAHAIEELGNQNSPAFNQALPMLDSL
                     IKAEVAEREVRSVNYQLRVAKFPVYRDLVGFDFSQSLVNEATVKQLHRCDFMEQAQNV
                     VLIGGPGTGKTHLATAIGTQAVMHLNRRVRFFSTVDLVNALEQEKSSGRQGQIANRLL
                     YADLVILDELGYLPFSQTGGALLFHLLSKLYEKTSVILTTNLSFSEWSRVFGDEKMTT
                     ALLDRLTHHCHILETGNESYRFKHSSTQNKQEEKQTRKLKIET"
     gene            complement(9924..10424)
                     /locus_tag="ACHKTW_001406"
     CDS             complement(9924..10424)
                     /locus_tag="ACHKTW_001406"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO6462461.1"
                     /translation="MDSEEPPNVRVACSGDIDEVVRLMHDAAAWMSAKGTPAWDVARI
                     DRTFAETFVLRSELLVASCSDGIVGCCTLSAEDPEFWPDALKGEAAYLHKLAVRRTHA
                     GRGVSSALIEACRHAARTQGCAKLRLDCHPNLRGLYERLGFTHVDTFNPGWDPTFIAE
                     RLELEI"
     gene            complement(10552..11391)
                     /gene="sul1"
                     /locus_tag="ACHKTW_001407"
     CDS             complement(10552..11391)
                     /gene="sul1"
                     /locus_tag="ACHKTW_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul1"
                     /protein_id="HFO6462462.1"
                     /translation="MVTVFGILNLTEDSFFDESRRLDPAGAVTAAIEMLRVGSDVVDV
                     GPAASHPDARPVSPADEIRRIAPLLDALSDQMHRVSIDSFQPETQRYALKRGVGYLND
                     IQGFPDPALYPDIAEADCRLVVMHSAQRDGIATRTGHLRPEDALDEIVRFFEARVSAL
                     RRSGVAADRLILDPGMGFFLSPAPETSLHVLSNLQKLKSALGLPLLVSVSRKSFLGAT
                     VGLPVKDLGPASLAAELHAIGNGADYVRTHAPGDLRSAITFSETLAKFRSRDARDRGL
                     DHA"
     gene            complement(11385..11732)
                     /locus_tag="ACHKTW_001408"
     CDS             complement(11385..11732)
                     /locus_tag="ACHKTW_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter QacE delta 1"
                     /protein_id="HFO6462463.1"
                     /translation="MKGWLFLVIAIVGEVIATSALKSSEGFTKLAPSAVVIIGYGIAF
                     YFLSLVLKSIPVGVAYAVWSGLGVVIITAIAWLLHGQKLDAWGFVGMGLIIAAFLLAR
                     SPSWKSLRRPTPW"
     gene            complement(11938..12726)
                     /locus_tag="ACHKTW_001409"
     CDS             complement(11938..12726)
                     /locus_tag="ACHKTW_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA5"
                     /protein_id="HFO6462464.1"
                     /translation="MGEFFPAQVFKQLSHARAVIERHLAATLDTIHLFGSAIDGGLKP
                     DSDIDLLVTVSAAPNDSLRQALMLDLLKVSSPPGDGGTWRPLELTVVARSEVVPWRYP
                     ARRELQFGEWLRHDILSGTFEPAVLDHDLAILLTKARQHSLALLGPSAATFFEPVPKE
                     HFSKALFDTIAQWNAESDWKGDERNVVLALARIWYSASTGLIAPKDVAAAWVSERLPA
                     EHRPLICKARAAYLGSEDDDLAMRVEETAAFVRYAKATIERILR"
     gene            complement(12784..13416)
                     /locus_tag="ACHKTW_001410"
     CDS             complement(12784..13416)
                     /locus_tag="ACHKTW_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002075262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B-3 chloramphenicol O-acetyltransferase
                     CatB3"
                     /protein_id="HFO6462465.1"
                     /translation="MTNYFDSPFKGKLLSEQVKNPNIKVGRYSYYSGYYHGHSFDDCA
                     RYLFPDRDDVDKLIIGSFCSIGSGASFIMAGNQGHRYDWASSFPFFYMQEEPAFSSAL
                     DAFQKAGNTVIGNDVWIGSEAMVMPGIKIGHGAVIGSRSLVTKDVEPYAIVGGNPAKK
                     IKKRFTDEEISLLLEMEWWNWSLEKIKAAMPMLCSSNIVGLHKYWLEFAV"
     gene            complement(13534..14007)
                     /gene="dfrA1"
                     /locus_tag="ACHKTW_001411"
     CDS             complement(13534..14007)
                     /gene="dfrA1"
                     /locus_tag="ACHKTW_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA1"
                     /protein_id="HFO6462466.1"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVLIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISTIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
     gene            14164..15177
                     /gene="intI1"
                     /locus_tag="ACHKTW_001412"
     CDS             14164..15177
                     /gene="intI1"
                     /locus_tag="ACHKTW_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012077405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HFO6462467.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATP
                     HTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLT
                     SER"
     gene            15380..15730
                     /locus_tag="ACHKTW_001413"
                     /pseudo
     CDS             15380..15730
                     /locus_tag="ACHKTW_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004636771.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3330 domain-containing protein"
     gene            15856..16416
                     /locus_tag="ACHKTW_001414"
     CDS             15856..16416
                     /locus_tag="ACHKTW_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFO6462468.1"
                     /translation="MTGQRIGYIRVSTFDQNPERQLEGVKVDRAFSDKASGKDVKRPQ
                     LEALISFARTGDTVVVHSMDRLARNLDDLRRIVQTLTQRGVHIEFVKEHLSFTGEDSP
                     MANLMLSVMGAFAEFERALIRERQREGIALAKQRGAYRGRKKSLSSERIAELRQRVEA
                     GEQKTKLAREFGISRETLYQYLRTDQ"
     gene            16419..19385
                     /locus_tag="ACHKTW_001415"
     CDS             16419..19385
                     /locus_tag="ACHKTW_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element TnAs3 family transposase"
                     /protein_id="HFO6462469.1"
                     /translation="MPRRSILSAAERESLLALPDSKDDLIRHYTFNDTDLSIIRQRRG
                     PANRLGFAVQLCYLRFPGVILGVDELPFPPLLKLVADQLKVGVESWNEYGQREQTRRE
                     HLSELQTVFGFRPFTMSHYRQAVQMLTELAMQTDKGIVLASALIGHLRRQSVILPALN
                     AVERASAEAITRANRRIYDALAEPLADAHRRRLDDLLKRRDNGKTTWLAWLRQSPAKP
                     NSRHMLEHIERLKAWQALDLPTGIERLVHQNRLLKIAREGGQMTPADLAKFEPQRRYA
                     TLVALATEGMATVTDEIIDLHDRILGKLFNAAKNKHQQQFQASGKAINAKVRLYGRIG
                     QALIDAKQSGRDAFAAIEAVMSWDSFAESVTEAQKLAQPDDFDFLHRIGESYATLRRY
                     APEFLAVLKLRAAPAAKNVLDAIEVLRGMNTDNARKLPADAPTGFIKPRWQKLVMTDA
                     GIDRRYYELCALSELKNSLRSGDIWVQGSRQFKDFEDYLVPPEKFTSLKQSSELPLAV
                     ATDCEQYLHERLTLLEAQLATVNRMAAANDLPDAIITESGLKITPLDAAVPDTAQALI
                     DQTAMVLPHVKITELLLEVDEWTGFTRHFTHLKSGDLAKDKNLLLTTILADAINLGLT
                     KMAESCPGTTYAKLAWLQAWHTRDETYSTALAELVNAQFRHPFAGHWGDGTTSSSDGQ
                     NFRTASKAKSTGHINPKYGSSPGRTFYTHISDQYAPFHTKVVNVGLRDSTYVLDGLLY
                     HESDLRIEEHYTDTAGFTDHVFALMHLLGFRFAPRIRDLGDTKLYIPKGDAAYDALKP
                     MIGGTLNIKHVRAHWDEILRLATSIKQGTVTASLMLRKLGSYPRQNGLAVALRELGRI
                     ERTLFILDWLQSVELRRRVHAGLNKGEARNALARAVFFNRLGEIRDRSFEQQRYRASG
                     LNLVTAAIVLWNTVYLERAAHALRGNGHAVDDSLLQYLSPLGWEHINLTGDYLWRSSA
                     KIGAGKFRPLRPLQPA"
     gene            complement(19420..19659)
                     /locus_tag="ACHKTW_001416"
                     /pseudo
     CDS             complement(19420..19659)
                     /locus_tag="ACHKTW_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001132032.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            20013..20615
                     /locus_tag="ACHKTW_001417"
     CDS             20013..20615
                     /locus_tag="ACHKTW_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ProQ/FINO family protein"
                     /protein_id="HFO6462470.1"
                     /translation="MGKNQEERKTPVIVVKKRRTFSPPSLSEKTDIIAPVFTEQTAES
                     APAGINSSAVETHIPEAPARKKKKKRHRFPRPSHWTREYTHECVEKIKALFPHLRAEG
                     GGFIPLKIGINNDISAFLAEHPETELTMDEWLCAVSCITSRRVYLQRTAVAGVPRYGL
                     DGHPKGQVSDSEAQSAGRRLATLEQKWLRMQAQQENISGQ"
     gene            complement(21267..21812)
                     /locus_tag="ACHKTW_001418"
     CDS             complement(21267..21812)
                     /locus_tag="ACHKTW_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001523466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6462471.1"
                     /translation="MLLSFSELDVLIYQGKVGGLFRERKNERVATDIDSRNKSSSSSH
                     EYDSVLSFQFICDNGKEINVNLTNWNINIFPGQELIIYFVENSKKNKKIPYMIKNKST
                     SEVTNNVQEMYKEFFYTSYFKLIIPFLLIISYFMSSISVAIMSFVLYVIIRRRIGKDF
                     FSEIKMKRLKKEDIMGLEHVI"
     gene            complement(21822..22109)
                     /locus_tag="ACHKTW_001419"
     CDS             complement(21822..22109)
                     /locus_tag="ACHKTW_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO6462472.1"
                     /translation="MTYTVKFRDDALREWLKLDKSVQQQFAKKLKKCSENPHIPSAKL
                     RGLKDCYKIKLRASGFRLVYQVIDDMLIIAVVAVGKRERSHVYNLASERMR"
     gene            complement(22106..22357)
                     /locus_tag="ACHKTW_001420"
     CDS             complement(22106..22357)
                     /locus_tag="ACHKTW_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001595120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFO6462473.1"
                     /translation="MPSIILSDTSASVSELKKNPMATVSAGEGYPVAILNRNQPAFYC
                     VPAELYERMLDAMDDQELVKLVTERSNQPLHDVDLDSYL"
     gene            complement(22444..22611)
                     /locus_tag="ACHKTW_001421"
     CDS             complement(22444..22611)
                     /locus_tag="ACHKTW_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000841011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6462474.1"
                     /translation="MAESGYGLAGLGMGKVKSINQYRLTPGFGGFTPVSYETTECRLI
                     CRWRGIRITEE"
     gene            complement(23306..24178)
                     /gene="repA"
                     /locus_tag="ACHKTW_001422"
     CDS             complement(23306..24178)
                     /gene="repA"
                     /locus_tag="ACHKTW_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HFO6462475.1"
                     /translation="MAALNQTSFTESTYRQVKNPNPVFTPREDAGTLKFCEKLMEKAV
                     GFTSRFDFAAHVAYARSRGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTL
                     AIECGLATESEAGKLSITRATRALKFLSELGLITYQTEYDPTIGCNIPTDITFTPALF
                     SSLDISEEAVASARRSRVEWENKLRKKQGLDALGMDELIARAWRFVRERFRSYQAELK
                     SHGMKRARARRDAGRTRQDIVTLVKRQLTREIAEGRFRGSLEAVKREIDRRVKERMIM
                     SRNNNYTRLATASP"
     gene            24881..25522
                     /locus_tag="ACHKTW_001423"
     CDS             24881..25522
                     /locus_tag="ACHKTW_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000335849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0140673 - transcription elongation-coupled
                     chromatin remodeling [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination NusG
                     family protein"
                     /protein_id="HFO6462476.1"
                     /translation="MCLSELQAGLNEGQPLFPTHALCIRRSQQHRSPSAGIDFRGAFR
                     ILNISELHQRNWYLAQYIPAGKNREHLFSWLSEQHVLPWTPLILKKVRRTDKVCGYRR
                     HIHAVFPGYFFLKADPERHSFTHLRRHSAFLDFVKMAGEIKTVREDIVQSLMKVYPDP
                     ALNPAAREELDAASTLWLTKARYQYLLRLDAQPLPESRIALLLHLVSDDGALT"
     gene            25663..26328
                     /locus_tag="ACHKTW_001424"
     CDS             25663..26328
                     /locus_tag="ACHKTW_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000154135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="traC"
                     /protein_id="HFO6462477.1"
                     /translation="MTILSQENPVILLLENMLEALSTAPDNINNERRRRRYLLNWLDT
                     ARQMREFRGMAEEFTTLRKLLATNSDIPVANTLKSLLESGNNAALYDLYRFRSALSAC
                     RSLGWQIGTCAWNDQLLSETLSRAQTGKRHILQLNSTRDTFSTTGRMLRPVAFSLIHP
                     ASLESSEVEEIFRDEGFILAHGRECSLNGSGRNMLSRILHVGHSGLPKAEWGIDHSNH
                     LLH"
     gene            26595..27215
                     /locus_tag="ACHKTW_001425"
     CDS             26595..27215
                     /locus_tag="ACHKTW_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000176498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold-containig protein"
                     /protein_id="HFO6462478.1"
                     /translation="MTLTLLTAYNAPYLAALTFVLLTGIAELIALLCGHSLSSAMDTP
                     DLPEGLTGEALDWLNIGRIPLLIVLCMLAGFFGISGILLQGLIIHLLQAPAPNILLAP
                     LCLLLTCPLVHRTGRLIAPWLPRDETTALTEEAFIGCMATITGYHAVAGSPCEGRFTD
                     RFGQVHYLLLEPEAGKEFRRGDKVIIVCRLSGSRWLGELNPWPDVL"
     gene            27242..28936
                     /locus_tag="ACHKTW_001426"
     CDS             27242..28936
                     /locus_tag="ACHKTW_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000482663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HFO6462479.1"
                     /translation="MFNLPGWLFTVIALVLTLFVIGLIFARLYRRASAEQAFVRTGLG
                     GQKVVMSGGAIVMPIFHETIPVNMNTLKLEVSRAAAESLITRDRMRVDVAVAFFLRVK
                     PSAEGISTAAQTLGQRTLTPEDLRSLVEDKFVDALRATAARMSMQDLQDARENFVQGV
                     QNTVAEDLSKNGLELESVSLTSFNQTAREHFNPDNAFDAEGLTLLTQETERRRRERNE
                     VEQDVEVAIREKNRDALSRRLEIEQQEAFMTLEQQQRVKTRTAEQSASIAAIEAERRR
                     EAESARILAERKIEEAEIERQQIVRTRQVEAEREVAIREIEQQQATEIASQARAIAVA
                     AKSEEQSQAEARASKALAEAVQAQQDVETTRQTAEADRTKQVALIAAAQEAETKAVEL
                     TVRAQAEKEAAELQAAAIIELAEATRQKGLAEAEAQRALNDAINVLSDEQTSLRFRLA
                     LLDALPAVIEKSTEPMKSIDGIKIIQVDGLNGGVTRGKTATTDISGGNLAEQAVTAAL
                     AYRTQAPLIDSLLKEVGLSGEGLTALTRPLVMPAPEKVPDVSEPVTAPDNSEPQQV"
     gene            29017..29259
                     /locus_tag="ACHKTW_001427"
     CDS             29017..29259
                     /locus_tag="ACHKTW_001427"
                     /inference="COORDINATES: protein motif:HMM:NF022090.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilI type IV pilus biogenesis protein"
                     /protein_id="HFO6462480.1"
                     /translation="MSAEKFRVVIYTNTCEKKVSYIPATEDINIAIMPFQTADNGLII
                     VCQNSRIIRRKERIPGSSCEWKDVTLAPKSRNRVQL"
     gene            29376..29711
                     /locus_tag="ACHKTW_001428"
     CDS             29376..29711
                     /locus_tag="ACHKTW_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001402868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6462481.1"
                     /translation="MRTITTREQLLINGRVHERIATHIVTGAHGYETLCTNGYNVRYN
                     KAQQLVESCEKIAEGKLPVTCPVCFAIWQDVHRFTHVDFDTQSGKSDFINTCHSEIIT
                     GNVSITGKK"
     gene            29868..30938
                     /locus_tag="ACHKTW_001429"
     CDS             29868..30938
                     /locus_tag="ACHKTW_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000730426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcpQ domain-containing protein"
                     /protein_id="HFO6462482.1"
                     /translation="MQKKSLTLLLPSLLSGCTAFNATPVPDTTPPALVFVDGQIRESA
                     AIIAHTQKRLLPPPVTRPVTPPRPTTTATPTTAVSSQSTALPPRPLLQATQLPALVMT
                     GKPGQSSVVYLREARQRSVSDWIRQLLPPGWQFRQQNAAIPALNKRLPVWSVNDQWHR
                     ALNTLLTGQQLYGHMDWNRKILTVTTTAAPPVDLTAPQGSQKTADTPRNPFRSTTGTA
                     ADQAQVPPAVSASRVQSTTGTSGTSAPRTTTPAPVVTKPVAPPPQKVTPPRQWKMEKG
                     TTLKDGLARWTSSEKCPHGTWTLRWETPVNYSIDAPLVFTGSFRQALEQLFALYTPVE
                     KPLYARTSTAQCLLRVTDRPQE"
     gene            30943..31380
                     /gene="pilM"
                     /locus_tag="ACHKTW_001430"
     CDS             30943..31380
                     /gene="pilM"
                     /locus_tag="ACHKTW_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis protein PilM"
                     /protein_id="HFO6462483.1"
                     /translation="MGWFIVFIMVITTTGNFWFTSQLSRSEHRHQAAENTQQAATFIR
                     YMNAINDYLHQHPERRTAGGRLTSAQLGIPGTDTVSHIISQQRVFVWATETPGLMAAL
                     REQSNDSALLGRVENGRLLDTAGRALSVTLPSVIPDHVILWMN"
     gene            31411..33030
                     /locus_tag="ACHKTW_001431"
     CDS             31411..33030
                     /locus_tag="ACHKTW_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilN family type IVB pilus formation outer
                     membrane protein"
                     /protein_id="HFO6462484.1"
                     /translation="MNKKILAIMIAAALLQGCASYRQLESTRKEAVAEQQRAREMMSD
                     LRTRTPVVKESSRQWINPRPVMDKNTGKTVPGCPIVIHTRQSITLQQVVQRITESCHI
                     PVRITPDVLTYLNTSTTGSTQQIQGALPPPDASGMVPLAAIGASDTPPSRLPGNTAAL
                     SELNASGLTTLLNAVSSRLGISWRYDGSHIIFYYLETQTFPITYMDSQVAYNATVVSG
                     TMSSNGSSGGSASDAISGDASNTQTTTVDMKSALYQDVKNAVTSMLTPGIGRMFMSTG
                     FITVTDNPQVLETVRAFIEKRNEEMKRQVVLNVEILSIRKTRNEQAGIDWNAVFSDRT
                     LGLSLGSTFTSAASDAITGGVSIVDGKLTGSKAFLKALSSQGDVSVVTQNSAVTKNLT
                     PVPMQIANQQSYIESVTTDTTANVGSSTSLNAATITTGFNMTLLPFILPDSQTLQLLY
                     SMSLSDKPVIENYESGGSKAQLPNVDLKTINQTVDLKSGQTVIISGFQQSGRRSGKQG
                     VGTPGFFGLGGGINSENDDTILVVLITPNII"
     gene            33051..34346
                     /gene="pilO2"
                     /locus_tag="ACHKTW_001432"
     CDS             33051..34346
                     /gene="pilO2"
                     /locus_tag="ACHKTW_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4b pilus protein PilO2"
                     /protein_id="HFO6462485.1"
                     /translation="MTDISPTGRQVFPLTAGRNVFLAGLEWKTLPSQYRHARDFARAQ
                     KADLFLACQYLSNEDADTHTMVATVSRRILPGKPRQCFSLALLILPLLEHGGYAITEL
                     TLPGETPRYSFVSVVDGVLVSDLVGSGEEVREARDTFLSINTEPTQGWIHYAPAAFSA
                     GDQNQHLPLASLTGSGKHPAAARLNPVSRGPQFITVSLIIALLGTAWYGWQYYERWQT
                     EKAAQAAVARQATEERITPPWPGLPETDAFIRGCADIWTSLPLSAAGWRYSLAECSTD
                     GGSGNLRASYTNTAGATVTDFIRRISDMTDSRPFIVMPEGKTGGIGLPVTFRLSENAV
                     TVDALPETAVLQERLTSLAQSMQLKLDWQEVNNSLTDENGQIIQPPWKEYDLQMQTTL
                     PAHHLAERLKEPSVRFISVTRQLENGRFHYQFTGKYYAR"
     gene            34336..34788
                     /gene="pilP"
                     /locus_tag="ACHKTW_001433"
     CDS             34336..34788
                     /gene="pilP"
                     /locus_tag="ACHKTW_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis protein PilP"
                     /protein_id="HFO6462486.1"
                     /translation="MPDKAKTLLFSVLLLPATAMATTGNAEQQNTQQIRIEQIQEETA
                     LYEAMLAREKIRHELRTLEQGVPAEAGRQQESISAVTTNVQALPSPRLTAIFGVHPQL
                     TARIELPGGSISDVKAGQNIPGTTYHVEKITPDSVVLEQSGTRKTLRP"
     gene            34873..36381
                     /locus_tag="ACHKTW_001434"
     CDS             34873..36381
                     /locus_tag="ACHKTW_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HFO6462487.1"
                     /translation="MLDPADLPPDDILDYVAIDTDNAGQLRIRVVEGKKHLRAVQEYL
                     TRLRARYQGRVGDFEFTTLDVIARLRQDATTTGDENAINPIQQKMLGYIRHSASLGAS
                     DLHMTPGRDNTDFTYVEARVHGELEVLDILRKEEGLELLGATYSGMTDVIKGTQFDPG
                     VPQDARLSEQYLKLAGLFGARYSHYPCVGGLYAVLRLIKDDSQQIPTFSMLGYHPDQE
                     CTLRRILQRPEGIITLSGPTGSGKSTTLRTASAAYLEQYGFNNTGGILLPRRRLFTIE
                     SPPEGRIPGAIQTAVMDTTRGWVDSIKSALRLDPDSILNGEIRDHDSAITAIKAAMTG
                     HLMLTTIHANDPINILERLEMEGVQARMIADPQLFIGLLSQRLVQLICPHCRHPWNDV
                     ASSRTKDERHLVETFCNADAVYLRNHEGCPHCWRGVTGRTVIAEVISPDARFFQLYRE
                     KGRIEAKTYWHRELGGMTRNQHLLGKINSGLVDPLAAHYISPIDEDSYTLLR"
     gene            36383..37483
                     /locus_tag="ACHKTW_001435"
     CDS             36383..37483
                     /locus_tag="ACHKTW_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000670135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HFO6462488.1"
                     /translation="MKFLEKLNSTELDLSALNSWTARKTFGLKRAEFYESLGGMIRDG
                     TPSLRALEFICNVETDFGTRKGTSGMYFLARECIASLKASGMLSGALPTWVPPEEAAL
                     IRNGEERGNIAEALFQVAKTMKARREMVSSLITLCLYPLLLLSLCVENMYNAHTRLIP
                     IVSAFAPEEKWSFQMKVLAAMSELVIGYGLWISGAIITLVIIIRFSLSRYTGPGRRLL
                     DKLPPWSLYQTFHGVNYLFSISSMLQINIPLSQALLRIEKSAEHNKWLKERIQAIRKN
                     VLSGQNLATAMRNSDYDFPSKICINKLLLNSEREDSVSAMNTYAGQWLEDAKNSVRKS
                     GLIITGISGGFVFFFILNMISAIYSISDMLKQ"
     gene            37557..38093
                     /locus_tag="ACHKTW_001436"
     CDS             37557..38093
                     /locus_tag="ACHKTW_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009447271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4 pilus major pilin"
                     /protein_id="HFO6462489.1"
                     /translation="MNFRARLSSYFSLKKDKEDDKGLTLLEIAGAMIIIAIVVALAMS
                     QMSSTMASSDNQRELKNLQTIATKMKQYKFQGRYTGTDYIKTLTESGALPADMIAGGN
                     KAKNAWGGDVTIAATADKYGYIITSNNVPKTNCVELINSLRSSSIFTKIMNTAPNTVD
                     PVTVCSSDKNNIKLETNS"
     gene            38147..38620
                     /locus_tag="ACHKTW_001437"
     CDS             38147..38620
                     /locus_tag="ACHKTW_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000870986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HFO6462490.1"
                     /translation="MKWFYLSSLFLFCLPATAAVNSNTLDSCFIQAGKRYSIDPGLLK
                     VIARKESSLNPRAINHNRNASGKIISTDYGLMQINSTHIPGLKRMGIIRSENELLSNV
                     CLNIQTGAWILARHFQQCGVNWECLGSYNAGFNKNNTHRRMKYARLIYAAYMQGR"
     gene            38639..39274
                     /locus_tag="ACHKTW_001438"
     CDS             38639..39274
                     /locus_tag="ACHKTW_001438"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HFO6462491.1"
                     /translation="MFLIILLVISATLTIQLLAIRVAGHYLMQVHTLRLPRHLSLLCV
                     VLALTATITHIIRGTDITGLCFLLLMAGCMTLMTLTDATSCRLPRPFTLTFLMLGVAF
                     RLWQGDAAIALACALFWFLLLYLLRRYTSYRKGVETLGLGDVFLIAGVAMWSSANSTP
                     WIIGIAATGTMVCMSLFPRSNTGAAIPFGPFICASLYIFTFFPGAIFHTGG"
     gene            39279..40643
                     /gene="pilV"
                     /locus_tag="ACHKTW_001439"
     CDS             39279..40643
                     /gene="pilV"
                     /locus_tag="ACHKTW_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001168069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shufflon system plasmid conjugative transfer
                     pilus tip adhesin PilV"
                     /protein_id="HFO6462492.1"
                     /translation="MQKDDDKGFVLFEILAGLIVIGIATPMIYSEIENWLNEQLYQSA
                     AYHADAYNTAARNYIADNNARLHSGSLPANFTADDLIRQGYLKQGFNHSPFGQSYITG
                     IRRNQTTGRLEALTCSTGGQTIKEEGLRSVAGQLPGLGGFISKNGTATGAFGAWTDKP
                     GDYGLTCSTGHIAVVMSGDDLQESDRLYRFQVAGRPELNQMHTAINMGGNNINNTGNI
                     NGQSATLKGDITSEDGWLITRNNKGWMNITHDGGFTMTDSQWIRAVNGKGITTTGEIK
                     GGKVSGGTVRSDGRLSTGEYLQLDKTATAGTKCSPDGLVGRTSTGAILSCQSGMWAGF
                     ESYPVGSPIPWPSATPPQGYLVMNGQSFSCSRYPQLARAYPGCKLPDLRGVFIRGWDN
                     GRGLDGDRNRHLLSYQADQSGVYHERGGWLKGHHSGMPYWAQGSTTEMRPKNIAFNYI
                     VKAS"
     gene            41258..42427
                     /locus_tag="ACHKTW_001440"
     CDS             41258..42427
                     /locus_tag="ACHKTW_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000486835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFO6462493.1"
                     /translation="MFQKNKTRKMSLSRALDKYLKTVSVHKKGHLQEFYRVNVIKRHP
                     MADRYMDEITTIDIASYRDQRLAQINPRTGRQITGNTVRLELALLSSLFNIARVEWGT
                     CRMNPVELVRKPKISNGRDRRLTSGEERRLSRYFRDKNPQLYVIFHLALETAMRQGEI
                     LSLRWEHLDLQHGVAHLPETKNGSPRDVPLSRKARNYLQILPQQINGNVFSYTSSGFK
                     SAWRTTLLDLKIENLHFHDLRHEAISRLFELGTLNVMEIAAISGHRSLNMLKRYTHLR
                     AYHLVSKLDARRKQTSKISPYFVPYPATVRNRNGVFIVTLHDFDLESRAETRELAISH
                     ASVLLLRALAQAAQRGERVPTPGELPANIDERVMICPLTNSQFMTKANSIFISSS"
     gene            42469..42759
                     /locus_tag="ACHKTW_001441"
     CDS             42469..42759
                     /locus_tag="ACHKTW_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001597008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6462494.1"
                     /translation="MKHLSSRAATADNNRIVTIEDVYTIAFSQPIHTESALGQRWMLI
                     HTGEYESASDAKRFLDTLVTGHCACMVTPSPVLDHDTWLRIRLLMGEIRDAK"
BASE COUNT        10102 a        12147 c        11335 g         9342 t
ORIGIN      
        1 tgaccaggcg caagcgcctg gaaatgctca attagggggc acctcagaaa acggaaaata
       61 aagcacgcta aggcatagct gaccttgcca ggcctgcttc gccctgtagt gacgcgatca
      121 acgggcagga aacattcccc tttcgtgcat ggcaggcgca cacgagttca gacagcacgg
      181 tttccatgcg cgccaagtcg gccatcttct cgcgcacgtc cttgagcttg tgttcggcca
      241 ggctgctggc ctcctcgcag tgggtgccat cgtcgagccg caacagctcg gcaatctcgt
      301 ccagactgaa ccccagccgc tgtgccgatt tcacgaattt cacccgaacc acgtccgcct
      361 ccccatagcg gcggatgctg ccgtaaggct tgtccggttc ccgcaacagg cccttgcgct
      421 gatagaagcg gattgtctcc acgttgaccc cggccgcctt ggcaaaaacg ccaatggtca
      481 ggttttccaa attattttcc atatcgcttg actccgtaca tgagtacgga agtaaggtta
      541 cgctatccaa tccaaattca aaagggccaa cgtatgtctg aaccacaaaa cgggcgcggt
      601 gcgctcttcg ccggcgggct ggccgccatt cttgcatcga cctgctgcct ggggccgcta
      661 gtactggtcg ccctgggctt ctccggtgct tggatcggca acctgacggt gctggaaccc
      721 tatcgaccgt tgttcatcgg cgcggcgcta gtggcgctgt tcttcgcctg gaagcggatt
      781 taccggcccg tgcaggcatg caagccaggt gaggtctgcg cgattccgca ggtgcgcgcc
      841 acctacaagc tgattttctg gatcgtggcc gtgctggtcc tggtcgcgct tggatttccc
      901 tatgtcgttc catttttcta ttaaccagga gttcatcatg aagaaactgt ttgcctccct
      961 tgccctcgcc gccgctgttg ccccggtgtg ggccgctacc cagaccgtca cgctagcggt
     1021 tcccggcatg acttgcgccg cctgcccgat cacagtcaag aaagcgctct ccaaggtcga
     1081 aggcgtgagc aaggtcgatg tgggcttcga gaagcgcgag gccgtcgtca cttttgacga
     1141 caccaaggcc agcgtacaga agctgaccaa ggccaccgca gacgccggct atccgtccag
     1201 cgtcaagcag tgagccagca agccaacgac aacagcgaga gccgcttcat gggactgatg
     1261 acacgcattg ccgataaaac cggcgcgctc ggcagcgtcg tttccgcgat gggctgcgcc
     1321 gcctgctttc cagccctcgc cagcttcggc gcggccatcg ggctgggctt cttgagccag
     1381 tacgagggac tgttcatcag ccgcctgctg ccgctgtttg ccgcgctggc cttcctggcg
     1441 aacgcgctgg gttggttcag tcatcggcaa tggctgcgca gtctgctcgg catgatcggc
     1501 ccggccatcg tgtttgcggc cacggtctgg ctgctcggca actggtggac ggcgaacctg
     1561 atgtacgtcg gcctggcctt gatgattggg gtgtcgatct gggacttcgt gtcgccggcg
     1621 catcgccgtt gcggaccgga cggctgcgaa ctccccgcca agcgcttgtg aaagacggct
     1681 gaccgtgcga cacggcggcc cacacgaata aggaacgatg gtatgagcac tctcaaaatc
     1741 accggcatga cttgcgactc gtgcgcagtg catgtcaagg acgccctgga gaaagtgccc
     1801 ggcgtgcaat cagcggatgt ctcctacgcc aagggcagcg ccaagctcgc cattgaggtc
     1861 ggcacgtcac ccgacgcgct gacggccgct gtagctggac tcggttatcg ggccacgctg
     1921 gccgatgccc cctcagtttc gacgccgggc ggattgctcg acaagatgcg cgatctgctg
     1981 ggcagaaacg acaagacggg tagcagcggc gcattgcata tcgccgtcat cggcagcggc
     2041 ggggccgcga tggcagcggc gctgaaggcc gtcgagcaag gcgcacgtgt cacgctgatc
     2101 gagcgcggca ccatcggcgg cacctgcgtc aatgtcggtt gtgtgccgtc caagatcatg
     2161 atccgcgccg cccatatcgc ccatctgcgc cgggaaagcc cgttcgatgg cggcatcgcc
     2221 gctaccacgc cgaccatcca gcgcacggcg ctgctggccc agcagcaggc ccgcgtcgat
     2281 gaactgcgcc acgccaagta cgaaggcatc ttggagggca atccggcgat cactgtgctg
     2341 cacggctccg cccgctttaa ggacaatcgc aacctgatcg tgcaactcaa cgacggcggc
     2401 gagcgcgtgg tggcattcga ccgctgcctg atcgccaccg gcgcgagccc ggccgtgccg
     2461 ccgattcccg gcctgaaaga cactccgtac tggacttcca ctgaagcgct ggtcagcgag
     2521 acgattccta agcgcctggc cgtgattggc tcatcagtgg tggcgctgga gctggcgcag
     2581 gcgttcgccc gactcggagc gaaggtgacg atcctggctc gcagcacgct gttcttccgc
     2641 gaagacccag ctataggcga agccgtcacg gccgcattcc gcatggaggg catcgaggtg
     2701 agggaacaca cccaggccag ccaggtcgcg tatatcaatg gtgaagggga cggcgaattc
     2761 gtgctcacca cggcgcacgg cgaactgcgc gccgacaagc tgctggtcgc caccggccgc
     2821 gcgcccaaca cacgcaagct ggcactggat gcgacgggcg tcacgctcac cccgcaaggc
     2881 gctatcgtca tcgaccccgg catgcgtaca agcgtggaac acatctacgc cgcaggcgac
     2941 tgcaccgacc agccgcagtt cgtctatgtg gcggcagcgg ccggcactcg cgccgcgatc
     3001 aacatgaccg gcggtgacgc ggccctgaac ctgaccgcga tgccggccgt ggtgttcacc
     3061 gacccgcaag tggcgaccgt aggctacagc gaggcggaag cgcaccatga cggcatcaaa
     3121 actgatagtc gcacgctaac gctggacaac gtgccgcgcg cgctcgccaa cttcgacacg
     3181 cgcggcttca tcaaactggt ggttgaagaa ggcagcggac gactgatcgg cgtgcaggca
     3241 gtggccccgg aagcgggcga actgatccag acggccgcac tggcgattcg caaccggatg
     3301 acggtgcagg aactggccga ccagttgttc ccctacctga cgatggtcga agggttgaag
     3361 ctcgcggcgc agaccttcaa caaggatgtg aagcagcttt cctgctgcgc cgggtgagga
     3421 caaggaggtg tgcgatgagc gcctacacgg tatcgcaact ggcccataac gctggggtga
     3481 gcgtacatat cgtgcgcgac tacctggtgc gcggcttgtt acggccggtg gcctgcacca
     3541 cgggcggcta cggcgtgttc gacgatgcgg ccttgcaacg gctgtgcttc gtgcgcgcgg
     3601 ccttcgaggc gggtatcggc ctggatgccc tggcgcggct gtgccgtgcg ctcgacgcag
     3661 cggacggcgc acaagccgca gcgcagcttg ccgtgctgcg ccagttggtc gagcggcggc
     3721 gcgcggcgtt ggcccatctg gacgcgcaac tggcctccat gccagccgag cgggcgcacg
     3781 aggaggcatt gccgtgaacg cccctgacaa actgccgccc gagacgcgcc aacccgtttc
     3841 cggctacctg tggggtgcgc tggccgtgtt gacctgcccc tgccatctgc cgattctcgc
     3901 cgccgtgctg gccgggacga ccgccggtgc cttccttggc gagcattggg gtgttgccgc
     3961 gctcgcgctg accggcttgt tcgttctggc cgtaacgcgg ctgctgcgcg ccttccgggg
     4021 cggatcatga cgagttcgca gcccgccgga tggacggcgg ccgagttggc gcaggcggcg
     4081 gcgcgcggac agcttgacct gcattaccag ccgctggtcg atctgcgcga tcaccggatc
     4141 gctggcgcgg aagcgttgat gcgctggcgg catccgaggc ttggcctgtt gccgcccggc
     4201 cagttcctgc cgctggccga gtcgttcggc ctgatgccgg aaataggcgc gtgggtgctg
     4261 ggcgaggcct gtcgccagat gcacaagtgg caaggaccgg catggcaacc gttccgtctt
     4321 gccatcaatg tgtccgccag ccaggttggg ccaacgttcg acgacgaggt aaagcgggtg
     4381 ctggccgata tggccctgcc cgccgagctt ctggagatcg aactgaccga atcggtcgca
     4441 ttcggcaatc cagccctgtt cgccagtttc gacgccttgc gcgccatcgg cgtgcgcttc
     4501 gccgccgacg acttcggcac cggctattcc tgcctgcaac atctgaaatg ctgccccatc
     4561 accacattga aaatcgacca atcctttgtc gccaggctcc cggatgatgc ccgtgaccaa
     4621 actatcgtgc gggcggtgat ccagctcgcg cacgggctgg gcatggatgt cattttcaga
     4681 agacgactgc accagttgat tgggcgtaat ggctgttgtg cagccagctc ctgacagttc
     4741 aatatcagaa gtgatctgca ccaatctcga ctatgctcaa tactcgtgtg caccaaagcg
     4801 aggtgagcat ggcgacggac accccacgga ttccagaaca aggcgtggcc actctgcctg
     4861 atgaggcttg ggagcgtgcg cgccgtcgtg cggagatcat cagtccgttg gcgcagtcgg
     4921 agacggtcgg gcacgaagcg gccgatatgg cggctcaggc gctgggcttg tctcggcgcc
     4981 aggtatacgt tctgatccgg cgtgcccggc aaggcagcgg cctcgtgacg gatctggtgc
     5041 ccggccagtc cggtggaggt aaaggtaagg ggcgcttgcc ggaaccggtc gagcgcgtca
     5101 tccacgagct actgcaaaag cggttcctga ccaagcagaa gcgcagccta gcggcctttc
     5161 accgcgaagt cactcaggtg tgcaaggctc aaaaactgcg agtgccggcg cgcaataccg
     5221 tggccttacg gatcgctagc cttgacccgc gcaaggtcat ccgccggcgg gaaggccagg
     5281 atgccgctcg tgacctacaa ggtgtgggcg gcgagcctcc tgccgtgacc gcgccgctgg
     5341 agcaggtgca gatagaccat acggtcatcg acctgatcgt ggtcgatgac cgcgaccggc
     5401 aacctattgg ccgcccgtac ctgaccctcg ccatcgacgt gttcacccgc tgcgtgctcg
     5461 gcatggtcgt cacgctggaa gcgccgtctg ccgtttcggt tggcctgtgc ctcgtgcatg
     5521 tcgcctgcga caagcgccct tggctggaag gactgaacgt ggaaatggat tggcagatga
     5581 gcggcaagcc cttgctgctc tacctagaca acgcggccga gttcaagagc gaggccctgc
     5641 gccggggttg cgagcagcat ggcatccggc tggactatcg cccgctggga cagccgcact
     5701 atggcggcat cgtggaacgg atcatcggca cggcgatgca gatgattcac gacgaactgc
     5761 cgggaacgac cttctccaac cctgaccagc gcggcgacta cgattccgaa aacaaggccg
     5821 ccctgacgct gcgcgagcta gagcgctggc tcacattggc ggtcggcacc taccacggtt
     5881 cggtgcacaa cggcctgctc caaccgccgg ccgcgcgctg ggccgaggcc gtggcgcgtg
     5941 tcggcgtacc ggccgtcgtc acacgcgcta cttcgttcct ggtcgatttt ctgccgatcc
     6001 tccggcgcac gctgacccgc accggctttg tcatcgacca catccactac tacgccgatg
     6061 cgctcaagcc gtggattgcg cggcgtgaac gctggccgtc ctttctgatc cggcgcgatc
     6121 cgcgcgacat cagccgtatc tgggtcctgg aaccggaggg acagcattac ctggaaattc
     6181 cctaccgtac cttgtcgcat ccggctgtca ccctctggga acaacggcag gcgctggcga
     6241 aactgcggca gcaagggcgc gaacaggtgg atgagtcggc gctgttccgc atgatcggcc
     6301 agatgcgtga gattgtgacc agcgcgcaga aggccacacg caaggcgcgg cgtgacgcgg
     6361 atcgccgcca gcacctcaag acatcagctc ggccggacaa gcccgttccg ccggatacgg
     6421 atattgccga cccgcaggca gacaacttgc cacccgccaa accgttcgac cagattgagg
     6481 agtggtagcc gtggacgaat atcccatcat cgacctgtcc cacctgctgc cggcggccca
     6541 gggcttggcc cgtcttccgg cggacgagcg catccagcgc cttcgcgccg accgctggat
     6601 cggctatccg cgcgcagtcg aggcgctgaa ccggctggaa gccctttatg cgtggccaaa
     6661 caagcaacgc atgcccaacc tgctgctggt tggcccgacc aacaatggca agtcgatgat
     6721 cgtcgagaag ttccgccgca cccacccggc cagctccgac gccgaccagg agcacatccc
     6781 ggtgttggtc gtgcagatgc cgtccgagcc gtccgtgatc cgcttctacg tcgcgctgct
     6841 cgccgcgatg ggcgcgccgc tgcgcccacg cccacggttg ccggaaatgg agcaactggc
     6901 tctggcactg ctgcgcaagg tcggcgtgcg catgctggtg atcgacgagc tgcacaacgt
     6961 gctggccggc aacagcgtca accgccggga attcctcaac ctgctgcgct tcctcggcaa
     7021 cgaactgcgc atcccgttgg ttggggtagg cacgcgcgac gcctacctag ccatccgctc
     7081 cgatgaccag ttggaaaatc gcttcgagcc gatgatgctg ccggtatggg aggccaacga
     7141 cgattgctgc tcactgctgg ccagcttcgc cgcttcgctc ccgctgcgcc ggccttcccc
     7201 aattgccacg ctggacatgg ctcgctacct gctcacacgc agcgagggca ccatagggga
     7261 actggcgcac ttgctgatgg cggcggccat cgtcgccgtg gagagcggcg aggaagcgat
     7321 caaccatcgc acactcagca tggcctgttg agttgcatct aaaattgacc cacttagggt
     7381 aaagatttgc gtcgaaattt gacccacgta tgacactgtt tcccgtctgg atatggcggg
     7441 agaaatcaag gagtgataaa cgtggcgata ttgagcgcaa ttcgacgctg gcattttcgc
     7501 gatggtgcgt cgattcggga aatagcccga cgaagcggcc tgtccaggaa caccgttcgc
     7561 aagtatttgc aaagcaaggt ggttgaaccg cagtacccag cgcgagacag cgttggcaag
     7621 ttaagtcctt ttgagcccaa gttaaggcag tggctctcca ccgagcacaa aaagacaaag
     7681 aagctgcgca gaaacctgcg cagcatgtac cgggatttgg tcgctttggg ctttaccggg
     7741 tcttatgacc gagtgtgtgc ctttgcccga cagtggaaag attccgaaca gttcaaggcg
     7801 caaacctcgg gcaagggttg tttcatcccc ttgcgctttg cttgtggcga agccttccaa
     7861 ttcgattgga gtgaggactt tgcccgcata gcgggcaaac aggtcaaact tcagattgcc
     7921 cagtttaagt tggcccacag ccgggccttt gtgcttcggg cttactacca gcaaaaacat
     7981 gaaatgctgt ttgatgccca ctggcatgcc tttcaaatct tcggtggcat tcccaagcgc
     8041 ggcatctacg acaacatgaa gaccgctgtg gattcggtgg ggcgtggcaa agagcgcagg
     8101 gtcaatcagc ggttcactgc catggtcagc cactacctgt ttgatgcgca gttctgtaat
     8161 ccagcatcgg gttgggagaa aggccagatt gagaagaacg tgcaggattc ccgccaacgc
     8221 ctgtggcaag gggcaccaga ctttcaaagc cttgctgatt tgaatgtgtg gcttgagcat
     8281 cgctgcaaag cgctgtggtc tgagctgcgc caccccgaat tggaccaaac cgtgcaagag
     8341 gcctttgccg atgaacaagg cgagttgatg gcgctaccca atgcctttga tgcattcgtg
     8401 gagcaaacca agcgagtcac ttcaacctgc cttgttcacc acgagggcaa tcgctacagc
     8461 gttcctgcca gttacgccaa cagggccatc agccttcgga tttatgcaga caagctggtg
     8521 atggctgccg aaggccaaca cattgccgag catccaagat tgtttggcag tggccacgct
     8581 cggcgtggcc acacacaata cgactggcac cattacttgt ctgtgcttca gaagaaacct
     8641 ggggcgttgc gcaatggtgc gccatttgct gaattgccac ccgcgttcaa gaagcttcaa
     8701 tccatcttgc tgcaacgccc cggcggtgac cgtgacatgg tggaaattct ggcccttgta
     8761 ttgcaccacg atgaaggtgc ggtactcagt gctgtggaat tggcattgga gtgtggcaag
     8821 ccatcgaagg agcatgtgct taatctgttg ggacgtttga ccgaagaacc tccacccaaa
     8881 ccgattccaa ttcccaaggg gttaaggctg acattggaac cacaggccaa cgtgaaccgc
     8941 tatgacagtt taaggagagc ccatgatgca gcatgaaggc catgtgagaa tcctcaaatc
     9001 cttgaaactc tttggcatgg cacacgccat tgaggagttg ggcaatcaga attcaccagc
     9061 atttaatcaa gccttgccca tgctggacag cttgattaaa gctgaagtgg cagagcgtga
     9121 agtacgttcg gtgaactatc aattgcgggt ggccaagttc cccgtgtatc gggacttggt
     9181 gggctttgac ttcagtcaaa gcctggttaa tgaggccacg gtcaaacaat tgcaccggtg
     9241 cgacttcatg gaacaagccc agaacgtggt gctgattggt gggccaggca caggcaagac
     9301 tcacctggcc acagccattg gtacacaagc agtgatgcac ttgaaccgac gggtgcgttt
     9361 cttctccacc gtggatttgg tcaatgcact ggagcaagag aaatcatctg ggcgtcaggg
     9421 acaaatcgca aaccgtctgt tgtatgccga tttggtgatt ctggatgagc tgggatattt
     9481 gccttttagc caaaccggtg gggcactgct gtttcacctg ctctcaaagc tgtacgaaaa
     9541 aaccagcgtg atactgacca ccaacttgag cttctcggaa tggagccgag tgtttggcga
     9601 tgaaaagatg acaacagcgt tgttggaccg actaacccac cactgccaca tcctggaaac
     9661 cggcaatgaa agttaccgct tcaaacacag ttcaactcag aataagcagg aggaaaaaca
     9721 gacccgcaaa ctgaaaatcg agacataatt ctgacaacaa ggggtgggtc aaaattcaat
     9781 gcaaatcccg ggtcaaattt gggtgcaaat caacagatat cgacaacctc tcgcgcaacc
     9841 aagacatcgc ggtcggactg caagtgatct tgaagccacg ggcccgtccc accccgacat
     9901 ggacctcgat gcccgaacgg acgttagatt tcgagttcta ggcgttctgc gatgaaggtt
     9961 ggatcccagc cgggattgaa agtgtcgacg tgggtgaatc cgagccgctc gtataggcca
    10021 cgcaggttcg ggtggcagtc gagccgcagc ttggcgcacc cctgcgttcg cgcggcatgg
    10081 cggcaagcct cgatcagcgc ggagctgaca ccccggcccg catgtgtccg tcgcaccgcg
    10141 agcttgtgca gatatgcggc ctcccccttg agggcgtcgg gccagaactc gggatcctcg
    10201 gccgacaagg tgcaacagcc gacgatgccg tcgctgcaac tcgcgactag gagctcggat
    10261 ctcaggacga aggtctccgc gaatgtccgg tcgatccgcg cgacgtccca ggcgggcgtt
    10321 cccttggcgg acatccacgc cgcagcgtcg tgcatcagcc gcacaacctc gtcgatatca
    10381 cccgagcagg cgacccgaac gttcggaggc tcctcgctgt ccattcgctc ccctggcgcg
    10441 gtatgaaccg ccgcctcata gtgcagtttg atcctgacga gcccagcatg tctgcgccca
    10501 ccttcgcgga acctgaccag ggtccgctag cgggcggccg gaaggtgaat gctaggcatg
    10561 atctaaccct cggtctctgg cgtcgcgact gcgaaatttc gcgagggttt ccgagaaggt
    10621 gattgcgctt cgcagatctc caggcgcgtg ggtgcggacg tagtcagcgc cattgccgat
    10681 cgcgtgaagt tccgccgcaa ggctcgctgg acccagatcc tttacaggaa ggccaacggt
    10741 ggcgcccaag aaggatttcc gcgacaccga gaccaatagc ggaagcccca acgccgactt
    10801 cagcttttga aggttcgaca gcacgtgcag cgatgtttcc ggtgcggggc tcaagaaaaa
    10861 tcccatcccc ggatcgagga tgagccggtc ggcagcgacc ccgctccgtc gcaaggcgga
    10921 aacccgcgcc tcgaagaacc gcacaatctc gtcgagcgcg tcttcgggtc gaaggtgacc
    10981 ggtgcgggtg gcgatgccat cccgctgcgc tgagtgcata accaccagcc tgcagtccgc
    11041 ctcagcaata tcgggataga gcgcagggtc aggaaatcct tggatatcgt tcaggtagcc
    11101 cacgccgcgc ttgagcgcat agcgctgggt ttccggttgg aagctgtcga ttgaaacacg
    11161 gtgcatctga tcggacaggg cgtctaagag cggcgcaata cgtctgatct catcggccgg
    11221 cgatacaggc ctcgcgtccg gatggctggc ggccggtccg acatccacga cgtctgatcc
    11281 gactcgcagc atttcgatcg ccgcggtgac agcgccggcg gggtctagcc gccggctctc
    11341 atcgaagaag gagtcctcgg tgagattcag aatgccgaac accgtcacca tggcgtcggc
    11401 ctccgcagcg acttccacga tggggatcgg gcgagcaaaa aggcagcaat tatgagcccc
    11461 atacctacaa agccccacgc atcaagcttt tgcccatgaa gcaaccaggc aatggctgta
    11521 attatgacga cgccgagtcc cgaccagact gcataagcaa caccgacagg gatggatttc
    11581 agaaccagag aaagaaaata aaatgcgatg ccataaccga ttatgacaac ggcggaaggg
    11641 gcaagcttag taaagccctc gctagatttt aatgcggatg ttgcgattac ttcgccaact
    11701 attgcgataa caagaaaaag ccagcctttc atgatatatc tcccaatttg tgtagggctt
    11761 attatgcacg cttaaaaata ataaaagcag acttgacctg atagtttggc tgtgagcaat
    11821 tatgtgctta gtgcatctaa cgcataattg agcggcgggc gcagcccgtc cgcttgaacg
    11881 ccgagttagg catcagatgc cctcggcgcg ggtcgatgca cttttcgcac atgccgctca
    11941 acgcaagatt ctctcaatcg ttgctttggc atatcgaacg aacgcggccg tctcttcgac
    12001 gcgcattgct aggtcgtcgt cctcgctacc caggtacgcc gcgcgtgcct tgcagatgag
    12061 gggccgatgc tcggcaggca aacgctccga tacccatgcg gcagcaacgt ccttaggagc
    12121 aatgagacca gttgaagcgc tgtaccaaat gcgagcaaga gcaagaacga cgttccgctc
    12181 gtcacccttc caatccgact ctgcattcca ctgggcaata gtgtcgaaaa gcgccttgga
    12241 gaaatgctcc ttcggcaccg gctcgaaaaa cgtggctgcg gatgggccta gaagcgcaag
    12301 gctgtgttgc ctcgccttgg tcagcaaaat cgcaagatcg tgatccagaa cggcaggctc
    12361 gaacgttccg gaaaggatgt cgtggcggag ccactcaccg aactgaagct cacgccgcgc
    12421 cggatagcgc caaggcacta cttcgcttcg agcgacaaca gttagctcca gcggtcgcca
    12481 tgttccgcca tcgcctggcg gtgatgagac tttcagcaaa tcgagcatta gcgcctgccg
    12541 gagcgaatcg ttaggtgcgg cgctgacggt cacgagcaag tctatgtcgc tgtccggctt
    12601 cagccctcca tcgatcgcag atccgaacag gtggattgtg tccagtgtcg cagccagatg
    12661 gcgctcgatc accgcgcgag cgtgggacag ctgcttgaaa acttgtgcag ggaaaaattc
    12721 acccatgatg cctaacgcct gaaataagcc gtgccgcgaa gcggcatcgg cttgattgaa
    12781 ttgttagacg gcaaactcga gccaatactt gtgcaggcca acaatattag acgagcacag
    12841 catgggcatt gccgctttga tcttctccag tgaccaattc caccactcca tctccagaag
    12901 caatgaaatt tcctcatcgg tgaagcgttt cttaatcttc ttagcgggat tgccgccaac
    12961 gatagcgtaa ggctccacat cttttgtcac caacgagcgg ctgcctatca ccgcaccgtg
    13021 cccgatcttg attccgggca tgaccattgc ctcagagccg atccaaacgt cattgccaat
    13081 gacagtatta cctgcttttt ggaaggcatc gagtgcgctt gagaatgcag gttcttcctg
    13141 catataaaag aacgggaaag atgatgccca gtcgtaccga tgcccctgat tgccagccat
    13201 gataaaggaa gccccactcc cgatagagca gaaactaccg atgatcaact tatcaacgtc
    13261 atcacggtcc ggaaacagat accgtgcgca gtcatcgaat gagtgcccat gatagtagcc
    13321 agagtaatag ctgtaccgcc caactttgat attggggttc ttcacttgct cagaaagcag
    13381 cttgcctttg aaggggctat caaagtagtt ggtcataaga gatcccgcgg tctgtgactt
    13441 tgccgtctaa cgcctggcac agcggatcgc aaacctggcg cggcttttgg cacaaaaggc
    13501 gtgacaggtt tgcgaatccg ttgctgccac ttgttaaccc ttttgccaga tttggtaact
    13561 ataatttatg ttagaggcga agtcttgggt aaaaactggc ctaaaattgc tggggatttc
    13621 aggaaagtaa acatcacctt ccggctcgat gtctattgta gatatatgta gtgtatctac
    13681 ttgatcgatc aggcttttgt atatctcccc accacctgaa acaatgacat gatccgttat
    13741 tttctttagg ttggttaaag catctttaat tgatggaaag atcaatacgt tctcattgtc
    13801 agatgtaaaa cttgaacgtg ttacgaccgc atactttcgg ttgggtaatg ctcccattga
    13861 ttcaaaagtc ttgcgtccaa ccaacagcca ttggttatag gtaatagctt taaacaggag
    13921 ctgttcacct ttggcactcc atggaatatc agggccattc ccgataactc cattcttcga
    13981 tatagctacc attagtgata gtttcacaat tcttcctcag aggttaactt tgttttaggg
    14041 cgactgccct gctgcgtaac atcgttgctg ctccataaca tcaaacatcg acccacggcg
    14101 taacgcgctt gctgcttgga tgcccgaggc atagactgta caaaaaaaca gtcataacaa
    14161 gccatgaaaa ccgccactgc gccgttacca ccgctgcgtt cggtcaaggt tctggaccag
    14221 ttgcgtgagc gcatacgcta cttgcattac agcttacgaa ccgaacaggc ttatgtccac
    14281 tgggttcgtg ccttcatccg tttccacggt gtgcgtcacc cggcaacctt gggcagcagc
    14341 gaagtcgagg catttctgtc ctggctggcg aacgagcgca aggtttcggt ctccacgcat
    14401 cgtcaggcat tggcggcctt gctgttcttc tacggcaagg tgctgtgcac ggatctgccc
    14461 tggcttcagg agatcggaag acctcggccg tcgcggcgct tgccggtggt gctgaccccg
    14521 gatgaagtgg ttcgcatcct cggttttctg gaaggcgagc atcgtttgtt cgcccagctt
    14581 ctgtatggaa cgggcatgcg gatcagtgag ggtttgcaac tgcgggtcaa ggatctggat
    14641 ttcgatcacg gcacgatcat cgtgcgggag ggcaagggct ccaaggatcg ggccttgatg
    14701 ttacccgaga gcttggcacc cagcctgcgc gagcagctgt cgcgtgcacg ggcatggtgg
    14761 ctgaaggacc aggccgaggg ccgcagcggc gttgcgcttc ccgacgccct tgagcggaag
    14821 tatccgcgcg ccgggcattc ctggccgtgg ttctgggttt ttgcgcagca cacgcattcg
    14881 accgatccac ggagcggtgt cgtgcgtcgc catcacatgt atgaccagac ctttcagcgc
    14941 gccttcaaac gtgccgtaga acaagcaggc atcacgaagc ccgccacacc gcacaccctc
    15001 cgccactcgt tcgcgacggc cttgctccgc agcggttacg acattcgaac cgtgcaggat
    15061 ctgctcggcc attccgacgt ctctacgacg atgatttaca cgcatgtgct gaaagttggc
    15121 ggtgccggag tgcgctcacc gcttgatgcg ctgccgcccc tcactagtga gaggtagggc
    15181 agcgcaagtc aatcctggcg gattcactac ccctgcgcga aggccatcgg tgccgcatcg
    15241 aacggccggt tgcggaaagt cctccctgcg tccgctgatg gccggcagca gcccgtcgtt
    15301 gcctgatgga tccaacccct ccgctgctat agtgcagtcg gcttctgacg ttcagtgcag
    15361 ccgtcttctg aaaacgacaa tggaggtggt agccgagggt gtggaaacac ccgactgcct
    15421 tgcgtggttg cggcaggcgg gttgcgacac ggtgcagggt ttcctgttcg ccaggccgat
    15481 gccggcggcg gccttcgtcg gcttcgtcaa ccaatggagg aacaccacca tgaacgccaa
    15541 tgaaccgagc accagttgct gcgtgtgctg caaggaaatc ccgctcgatg ccgccttcac
    15601 gccggaaggg gccgagtacg tggagcattt ctgcgggctg gagtgctatc agcgcttcca
    15661 ggcgcgggcc agcactgcga ccgaaaccag cgtcaaaccg gacgcttgtg attcgccgcc
    15721 gtcaggttga ggcataccct aacctgatgt cagatgccat gtgtaaattg cgtcaggata
    15781 ggattgaatt ttgaatttat tgacatatct cgttgaaggt catagagtct tccctgacat
    15841 tttgcaggga attccatgac tggacagcgc attgggtata tcagggtcag caccttcgac
    15901 cagaacccgg aacggcaact ggaaggcgtc aaggttgatc gcgcttttag cgacaaggca
    15961 tccggcaagg atgtcaagcg tccgcaactg gaagcgctga taagcttcgc ccgcaccggc
    16021 gacaccgtgg tggtgcatag catggatcgc ctggcgcgca atctcgatga tttgcgccgg
    16081 atcgtgcaaa cgctgacaca acgcggcgtg catatcgaat tcgtcaagga acacctcagt
    16141 tttactggcg aagactctcc gatggcgaac ctgatgctct cggtgatggg cgcgttcgcc
    16201 gagttcgagc gcgccctgat ccgcgagcgt cagcgcgagg gtattgcgct cgccaagcaa
    16261 cgcggggctt accgtggcag gaagaaatcc ctgtcgtctg agcgtattgc cgaactgcgc
    16321 caacgtgtcg aggctggcga gcaaaagacc aagcttgctc gtgaattcgg aatcagtcgc
    16381 gaaaccctgt atcaatactt gagaacggat cagtaaatat gccacgtcgt tccatcctgt
    16441 ccgccgccga gcgggaaagc ctgctggcgt tgccggactc caaggacgac ctgatccgac
    16501 attacacatt caacgatacc gacctctcga tcatccgaca gcggcgcggg ccagccaatc
    16561 ggctgggctt cgcggtgcag ctctgttacc tgcgctttcc cggcgtcatc ctgggcgtcg
    16621 atgaactacc gttcccgccc ttgttgaagc tggtcgccga ccagctcaag gtcggcgtcg
    16681 aaagctggaa cgagtacggc cagcgggagc agacccggcg cgagcacctg agcgagctgc
    16741 aaaccgtgtt cggtttccgg cccttcacca tgagccatta ccggcaggcc gtccagatgc
    16801 tgaccgagct ggcgatgcaa accgacaaag gcatcgtgct ggccagcgcc ttgatcgggc
    16861 acctgcggcg gcagtcggtc attctgcccg ccctcaacgc cgtcgagcgg gcgagtgccg
    16921 aggcgatcac ccgtgctaac cggcgcatct acgacgcctt ggccgaacca ctggcggacg
    16981 cgcatcgccg ccgcctcgac gatctgctca agcgccggga caacggcaag acgacctggt
    17041 tggcttggtt gcgccagtct ccggccaagc caaattcgcg gcatatgctg gaacacatcg
    17101 aacgcctcaa ggcatggcag gcactcgatc tgcctaccgg catcgagcgg ctggttcacc
    17161 agaaccgcct gctcaagatt gcccgcgagg gcggccagat gacacccgcc gacctggcca
    17221 aattcgagcc gcaacggcgc tacgccactc tcgtggcgct ggccaccgag ggcatggcca
    17281 ccgtcaccga cgaaatcatc gacctgcacg accgcatcct gggtaagctg tttaacgctg
    17341 ccaagaataa gcatcagcag cagttccagg cgtcaggcaa ggccatcaac gccaaggtac
    17401 gtctgtacgg gcgcatcggt caggcgctga tcgacgccaa gcaatcaggc cgcgatgcgt
    17461 ttgccgccat cgaggccgtc atgtcctggg attcctttgc cgagagcgtc accgaggcgc
    17521 agaagctcgc gcaacccgat gacttcgatt tcctgcatcg catcggcgag agctacgcca
    17581 ccctgcgccg ctatgcaccg gaattccttg ccgtgctcaa gctgcgggcc gcgcccgccg
    17641 ccaaaaacgt gcttgatgcc attgaggtgc tgcgcggcat gaacaccgac aacgcccgca
    17701 agctgccagc cgatgcaccg accggcttca tcaagccgcg ctggcagaaa ctggtgatga
    17761 ccgacgccgg catcgaccgg cgctactacg aactgtgcgc gctgtccgag ttgaagaact
    17821 ccctgcgctc gggcgacatc tgggtgcagg gttcacgcca gttcaaggac ttcgaggact
    17881 acctggtacc gcccgagaag ttcaccagcc tcaagcagtc cagcgaattg ccgctggccg
    17941 tggccaccga ctgcgaacaa tatctgcatg agcggctgac gctgctggaa gcacaacttg
    18001 ccaccgtcaa ccgcatggcg gcagccaacg acctgccgga tgccatcatc accgagtcgg
    18061 gcttgaagat cacgccgctg gatgcggcgg tgcccgacac cgcgcaggcg ctgatagacc
    18121 agacagccat ggtcctgccg cacgtcaaga tcaccgaact gctgctcgaa gtcgatgagt
    18181 ggacgggctt cacccggcac ttcacgcact tgaaatcggg cgatctggcc aaggacaaga
    18241 acctgttgtt gaccacgatc ctggccgacg cgatcaacct gggcctgacc aagatggccg
    18301 agtcctgccc cggcacgacc tacgcgaagc tcgcttggct gcaagcctgg catacccgcg
    18361 acgaaacgta ctcgacagcg ttggctgaac tggtcaacgc tcagtttcgg catccctttg
    18421 ccgggcactg gggcgatggc accacatcat catcggacgg acagaatttc cgaaccgcta
    18481 gcaaggcaaa gagcacgggg cacatcaacc caaaatatgg cagcagccca ggacggactt
    18541 tctacaccca catctccgac caatacgcgc cattccacac caaggtggtc aatgtcggcc
    18601 tgcgcgactc aacctacgtg ctcgacggcc tgctgtacca cgaatccgac ctgcggatcg
    18661 aggagcacta caccgacacg gcgggcttca ccgatcacgt cttcgccctg atgcacctct
    18721 tgggcttccg cttcgcgccg cgcatccgcg acctgggcga caccaagctc tacatcccga
    18781 agggcgatgc cgcctatgac gcgctcaagc cgatgatcgg cggcacgctc aacatcaagc
    18841 acgtccgcgc ccattgggac gaaatcctgc ggctggccac ctcgatcaag cagggcacgg
    18901 tgacggcctc gctgatgctc aggaaactcg gcagctaccc gcgccagaac ggcttggccg
    18961 tcgcgctgcg cgagttgggc cgcatcgagc gcacgctgtt catcctcgac tggctgcaaa
    19021 gcgtcgagct acgccgccgc gtgcatgccg ggctgaacaa gggcgaggcg cgcaatgcgc
    19081 tggcccgtgc cgtgttcttc aaccgccttg gtgaaatccg tgaccgcagt ttcgagcagc
    19141 agcgctaccg ggccagcggc ctcaacctgg tgacggcggc catcgtgctg tggaacacgg
    19201 tctacctgga gcgtgcggcg catgcgttgc gcggcaatgg tcatgccgtc gatgactcgc
    19261 tattgcagta cctgtcgcca ctcggctggg agcacatcaa cctgaccggt gattacctat
    19321 ggcgcagcag cgccaagatc ggcgcgggga agttcaggcc gctacggcct ctgcaaccgg
    19381 cttagcgtgc tttattttcc gttttctgag acgaccccta cccgtattgt ttttaagagc
    19441 agcaaaattc atggccggcg aatctcccgg aatacagtca cactgtaacc atgcatcata
    19501 ctcacgcatt tcccggagaa tatcattggc caggttatgt agtgccatgg ttgctggctt
    19561 tttcagtcca cagaccgtct ttactgcagt ctcttcctca ctgctaagct cccgaatcac
    19621 tttgccccgg ggattcttct ttacaagtat cattggcata atgaaagcac tccttatgta
    19681 catgcttaaa cagtaagtaa aatatacaaa gaacacccca tgaaacaaag gcagagatga
    19741 actgtgcgcc ccgatatctt cttctgatat cctttcaaat gaattgtttt tatttatatc
    19801 atgacaacat taaagtgaga gaatatatca accatgtttc tgtcagggtt cagtagaata
    19861 tcaattcgac tgtcacgcca taactgttac tacctcgagt ataaatactc agagaacgga
    19921 cttattagcc atgaaaaatc acctgatggg aaatttacta atttatgaat tgtgatatta
    19981 ttaaagagta aaaccagcca gccgaggtat ttatgggaaa aaatcaggaa gaaaggaaaa
    20041 ctcccgtcat tgtggtgaaa aaacgtcgta ctttttcgcc accgtcctta tccgaaaaaa
    20101 cagatattat cgcacctgta tttactgaac agacggcaga atcagccccc gccggaataa
    20161 attcatctgc cgtcgaaaca catatccccg aagcgccagc caggaagaag aaaaagaaaa
    20221 gacaccgttt tccacgtccc tcccactgga cccgtgagta tactcacgag tgcgtggaaa
    20281 aaataaaggc cctgtttcct catcttcgtg ctgaaggtgg tggatttatt cccctgaaaa
    20341 ttggcataaa caatgatatt tcagcatttc tggcagaaca cccggagaca gaactgacca
    20401 tggacgaatg gctctgtgca gtgtcctgca tcacatcccg ccgcgtatat ctgcagcgga
    20461 cagctgtcgc cggcgtccca cgttatggcc tggatggtca ccccaaaggg caggtctcag
    20521 actctgaagc acagagtgcc ggtcgcagac tggcgacatt agaacaaaag tggctcagga
    20581 tgcaggcaca gcaggagaat atttctgggc aataaacact cctgataaca ggtggacatt
    20641 acgtatcaga aaaaatggga agagaatgtt atgtgtgatt cagaagcgga gaagatttta
    20701 ttcatcctga ataaacatga acgggcatac agacgcctga tccccgtata tattttttta
    20761 gctatagtat tatggatttt acctattctc ctgattgtat acctgtattt ctgtatagtt
    20821 gacaatgagt ttctcagaat cggtttctcg aagtgacgag cgatcagaat ggagacaaat
    20881 aaattcggga aacgaacctt cacggtaagt tttgtcacat ttatcatccc gaaacatgag
    20941 aattatcaca aaaactaccc acccgaccaa cagtagcacc gggagcagca ttataaaatc
    21001 atgtacagag ttgcgtgacg acataataag ttctcaatat gctctttact ggattatacc
    21061 agtgatacag ttttttgata ctgcacactt gacatcctcc cccaccttcg cacttcgtga
    21121 ctcaggaggg ggattcccat tagtgggtga cggctgattg ctcagcgtct ggttcctgct
    21181 tcaacgggcg gtctgaaaac accgtatagc gcgggagtac accctaccgc gataactaga
    21241 cggtccctta taagaaccag tcgcccctat attacgtgct ctaaccccat gatatcttct
    21301 ttttttaacc ttttcatttt tatttctgaa aagaaatctt ttcctattct tcttctgatt
    21361 atgacatata aaacgaatga catgatagcc acagatatgg atgacataaa gtaagaaatg
    21421 attaacagga aaggtattat cagtttgaag tatgatgtat aaaaaaactc tttatacatt
    21481 tcttgaacat tattagtcac ttccgatgtg cttttgtttt ttatcatgta tggtattttt
    21541 ttatttttct ttgagttttc cacaaaataa ataataagct cttgacctgg aaatatgtta
    21601 atgttccaat ttgttaaatt cacatttatt tctttgccat tatcacaaat aaattgaaat
    21661 gataaaactg aatcatattc atgactgctt gatgatgact tgtttctgga atcaatgtct
    21721 gtagcaaccc tttcgttttt tctttcacga aataaacccc caaccttccc ctgataaata
    21781 agaacatcta actcactaaa acttaaaagc atttacaccc cctatctcat tctctcgctg
    21841 gcgagattgt aaacatgact acgttcacgt ttacctactg cgacaaccgc aataattaac
    21901 atgtcatcaa tcacctgata gaccagacga aaacctgatg cgcgaagttt tattttgtag
    21961 caatctttta atcctcgtag ttttgctgaa ggtatatgag gattttcgct gcattttttt
    22021 agtttttttg cgaactgttg ttgaacggat ttatccagtt tcagccattc cctgagggca
    22081 tcatccctga atttaaccgt ataagtcata aataactatc caggtccaca tcatgtagtg
    22141 gctgattgct gcgttcagta accagtttca ccagctcctg gtcatccata gcatcaagca
    22201 tcctttcata caattcagca ggaacacagt agaatgcagg ctgattccgg ttaagaatgg
    22261 caacaggata cccctctccg gcactgactg tcgccattgg attttttttc agttcactga
    22321 cacttgcact ggtgtcagac aaaatgatac tgggcataac acacctctga gactgtttaa
    22381 caggtcttca ccttacaccc tgaaagactg cttaacaagt ctgtttgatg ttaatgtgta
    22441 ctgtcactct tcagttatcc tgatgccgcg ccagcggcat ataaggcggc actcagttgt
    22501 ttcatatgat accggagtaa aaccgccgaa gcccggcgtc agccggtact gattgataga
    22561 tttcacctta cccatcccca gccctgctaa gccatacccg ctttcagcca tgagagagtt
    22621 tctgtgcgcg gtcggagtgg tcccgacgag ggtttacccg aagtcggggc gtatctccgc
    22681 gttagcgggc cgtcagggcc gcttacgagc gtgtattcag tacgtccagc cagaagacta
    22741 acagcgatga tgatgtttag ttacaacatt cataattaaa agcgatgttg ttcccgccct
    22801 tcgggcgggg gcggcctcgc ttttcagtta tgagaggtga gcacggtgct gcgtttgtgt
    22861 gatgttttta ttcctgatgt ctttttgctg tggtttcaga acggtgaggt tcggggcccg
    22921 cgcgttagcg tgggaggtgc atatttgctt gtccacagga caggcactga ataggttttc
    22981 ttttcaaatg gaagtaatta agtagtttaa aggaaataca gtactggggt ttaaaagaaa
    23041 cattgcaccc tgtaacgcct gcggctggcg ctttatagca ttaacggttg taaccttacg
    23101 gagaagtccc ttgcagttaa atgtggataa acagaattcc ccgtctgtga gacgtgtttt
    23161 gcgtgaaaaa cagggggagc aaggcgagcc ggaaggcaga tgatgaggct gttttttgtg
    23221 tgctgtgctg cttttttgtg cgccgccggg cttcagacgt gcggaagcct ccggcgcagg
    23281 ccggattatt cagatgaggt caaactcagg gtgaggcagt ggccagccgt gtgtagttgt
    23341 tgttccgcga cattatcatg cgttccttca cgcgacggtc gatttcgcgc ttcacggcct
    23401 ccaggcttcc gcggaaacgc ccttccgcga tttcacgtgt cagctgccgt ttcaccaggg
    23461 tgacaatgtc ctgacgggtc ctgcctgcat cgcggcgcgc acgggcacgt ttcatgccgt
    23521 gggacttaag ctctgcctga taactacgga aacgctcgcg cacaaaacgc caggctctcg
    23581 ctatcagttc atccataccc agcgcatcca gcccctgctt tttgcgcagc ttgttttccc
    23641 attccacgcg gctgcggcgt gcggaagcca ccgcctcttc tgatatatcg agtgaggaaa
    23701 acagtgccgg cgtgaacgtg atgtcggtcg ggatgttaca gccaattgtc gggtcgtatt
    23761 ccgtctggta ggttatcagc cccagctcag acaggaactt cagggcacgg gtggcacggg
    23821 tgatggagag tttcccggcc tcagactccg tcgccagtcc gcactcaatg gccagcgtgg
    23881 tgatggagca ctggacgcgg ttggccagcg gatcatagtg gaaacacagc ccctgcagca
    23941 gcgcatcaat cgcccggcgg cgcagcaccg gcggcatacg ccggcgcagg ccgcgcgaac
    24001 gggcatacgc cacatgcgcg gcaaaatcaa aacgggaggt gaagcccacc gccttttcca
    24061 tcagtttttc gcagaacttc agcgttccgg catcttcacg gggtgtgaac accggattcg
    24121 ggttctttac ctgacggtat gtgctttcag taaatgaggt ctggttcagg gccgccacaa
    24181 gctgtcgctt atggcggaaa cagaatgccg tgcgttaata cactgcgaag ggttgaatct
    24241 ctgacacggt ttcatatata cttatcccgt attctgtgag gcccccataa tcttctgtgc
    24301 gttccgccaa gttcgacagg aagattatgg gggttttcct ttatggcctt ctgaaacttt
    24361 tttcagcctt ccggccccgg caaactgccg tcgtttctgc catacatcct gaatgcctta
    24421 tggcgcattc tgccgttttc tggctttccg gaccgcgagg atcccaaaag ccactgaata
    24481 acgatccgca atatacccct tttccaccgc tttgaaaagc cgacacgatt tctctgattt
    24541 accaggcctc tcattaacca cctaaaaaac gtcgtttttt tgaactatgt caaaaaaata
    24601 gacaattcca caaacgatct tttatgctgt gtttttcatc atttttagta gaagagatcg
    24661 cctaaaattt ttaaacgatc taatcctcca gacaccgcac acaaatcagc ataatatgct
    24721 gctttatcaa atataacgat ctatttaact gcttatcttc actcttgcca cagatacatc
    24781 cttatgtaat atatttgcca gccattaatc ctgtttgcag gagttgtcgg ttttcgcccg
    24841 cctcgaaacc gtccacaagg cttacaagag tgcgtaaatc atgtgcctgt cagaactgca
    24901 ggccgggctg aacgagggtc agccgctgtt tccgacacat gctttatgta ttcgccgttc
    24961 acaacaacat cgaagcccct ctgcggggat cgattttcgc ggagcgttcc ggattttgaa
    25021 tatcagtgaa ttacatcaac gcaactggta tctggctcag tacatccctg ccggcaaaaa
    25081 ccgcgaacat ctcttttcct ggctttccga acaacacgtc cttccctgga cacccctgat
    25141 actgaaaaag gtccgccgca cggataaagt ctgtggttac cgtcgacata tacacgccgt
    25201 gtttcccggt tactttttcc tgaaagcaga ccccgaacgc cacagcttca ctcaccttcg
    25261 ccgtcacagt gcctttctgg acttcgtgaa aatggccggt gaaatcaaaa ccgtcaggga
    25321 ggatatcgtt cagtctttga tgaaagtgta cccggatcct gcgctcaatc cggcagccag
    25381 ggaggagctg gatgctgcct ccaccctctg gctgacaaaa gcgcgttacc agtacctcct
    25441 ccggcttgat gcacagcctc tgccggagtc gaggatcgcc ctgctcctgc acctggtatc
    25501 tgatgacgga gcgttgacat gaaaattgct gtcttaattt gacgactttt cccggccttt
    25561 atggccggga tttttttccc ttctgtttcc acaatgcccg gcaccggtga cacctgaggg
    25621 tgtacggaga gcggctgtcg ggcatttttt caggacgcct ttatgaccat tctatcccag
    25681 gaaaaccctg tcattctgtt actggagaat atgctcgaag cactctccac cgccccggac
    25741 aacataaaca acgaacggcg tcgccggcgt tacctgctga actggcttga cactgcccgt
    25801 cagatgcgcg agttcagagg aatggctgaa gagttcacta ccctgcgaaa actgctggcc
    25861 accaattcag acataccggt agcaaacacc ctgaaatcac tgctggaaag tggcaataac
    25921 gcagcattat acgacctgta ccgcttccgc tcggctcttt ccgcgtgcag aagtctggga
    25981 tggcagattg gcacgtgcgc atggaacgac cagctgctga gtgagacact ttcccgtgcg
    26041 caaacaggta aacgtcatat cctgcagctg aacagtacca gagacacctt ttccaccacg
    26101 ggcagaatgc tcagaccggt ggcattttca ttaattcatc cggcatccct cgaatcttca
    26161 gaagtggaag agatattccg ggatgaaggt tttatactcg cgcacggacg cgaatgcagc
    26221 ctgaacggca gcggcagaaa catgctgtca cgcattcttc atgtggggca ttcaggttta
    26281 ccgaaagccg aatggggcat agatcacagc aatcacctgt tacactgaaa taccgggaca
    26341 gccacaacgg cagccactct ccgctgactt tttttcgcaa atcgcggttt gctgatgtgg
    26401 atgtcccatt ttttattatc cgtcctctcc ggttgtcata aagcgcattc cggcgtgagg
    26461 cttctgttgt tatcatcccc tcctgcagac atccccttgt cttctgatga ctgacgatat
    26521 gcttcttctg acaattaaag aagcggaagc ttcacgccat aatgttgttt gactgacctg
    26581 ccacaggagg aaaaatgaca ctgacgttac ttaccgcgta taacgcaccc tatctggcag
    26641 ccctgacttt tgttctgctt actggtatcg ctgaacttat cgccctgctc tgcggacact
    26701 ccctctcttc agctatggat acacctgatt taccggaggg gctgacagga gaggcgctgg
    26761 actggctgaa catcggacgg atccccctgc tgatcgtcct ctgtatgctg gcaggttttt
    26821 ttggtatatc cggcattctc ctgcagggtc ttattattca tctgctgcag gctccagccc
    26881 cgaacattct ccttgcacca ctttgcctgc tgctgacatg tcctctcgta caccgcaccg
    26941 gcagactgat tgccccctgg ttaccccgtg atgaaacaac ggcgctcacc gaggaggctt
    27001 tcatcgggtg tatggccacc atcaccggtt atcacgcagt ggcgggttcc ccctgtgaag
    27061 gtcgcttcac agaccgcttt gggcaggttc attacctgtt gctggagccg gaggccggga
    27121 aagaattcag acgaggagac aaagtgatta ttgtctgccg tctttccggt tcccgctggc
    27181 tgggcgaact gaatccctgg ccggatgttc tttaatcccg acatatacag aggttctcac
    27241 tatgtttaat cttccgggat ggttgtttac cgttattgcg ctggtcctca ccctgttcgt
    27301 tatcgggctg atatttgccc gcctgtaccg tcgggcatcg gcagagcagg cctttgtccg
    27361 cacaggtctg ggaggccaaa aagtggtaat gagtggcggc gcaattgtta tgcccatttt
    27421 tcacgaaaca attcccgtca acatgaacac cctgaagctg gaagtcagtc gtgcagcggc
    27481 tgaaagtctc attacccgcg accgcatgcg tgttgatgtt gccgttgcct tttttcttcg
    27541 ggtaaaacca tcagccgaag gaatatccac tgcagcccag acgctgggac aacgcacgct
    27601 cacacctgaa gatctgcgta gtctggtcga ggataaattt gttgatgccc tgcgggccac
    27661 ggcggccaga atgagcatgc aggatttaca ggatgcccgc gagaactttg ttcagggtgt
    27721 gcagaacacc gttgcggaag acttatccaa aaacggtctg gagcttgaaa gtgtgtcgct
    27781 gacaagtttc aaccagactg cacgggaaca ttttaatcct gacaatgcct ttgatgctga
    27841 aggcctgacc cttctgaccc aggaaacaga acgtcgtcgc cgcgaacgta acgaagttga
    27901 gcaggatgtt gaagtggcca tcagggaaaa aaaccgggat gcactctccc gacgtctgga
    27961 gattgaacag caggaagcct tcatgactct ggagcaacaa caacgggtaa aaacccgtac
    28021 ggcagagcag agtgccagta ttgctgctat tgaagctgaa cgacgtcgtg aagcagaatc
    28081 cgcgcgaatt ctggctgaac gtaagataga ggaagccgaa atcgaacgac agcagatagt
    28141 gcgtacccgc caggtcgaag ccgaacggga agtagccata cgggaaattg agcaacagca
    28201 ggcaaccgaa atcgccagcc aggccagagc cattgccgtg gcggcaaaat cggaagagca
    28261 gtcacaggca gaagcccggg caagtaaagc actggctgaa gccgtacagg cccagcagga
    28321 tgtggaaacc acccgccaga ctgcggaggc cgaccgtacc aaacaggttg ccctgattgc
    28381 tgctgctcag gaagctgaaa cgaaggcggt tgaactgacg gttcgcgccc aggctgaaaa
    28441 agaagcagcc gaactccagg cagcagcaat tattgaactg gcggaggcca cccgtcagaa
    28501 aggtctggca gaagcggaag cacagcgcgc actgaacgat gccattaacg tgctttcgga
    28561 tgagcagacc agccttcggt tccgtcttgc tctgctggat gcattacctg ccgtcataga
    28621 gaagtccaca gaaccaatga aatccattga cggcattaaa atcattcagg tggatggcct
    28681 gaatggcggt gttacccgcg gtaaaacagc aacaaccgac ataagtggcg gaaatctcgc
    28741 tgaacaggcg gtaaccgccg ctctggctta ccgaacacag gctccgctca tcgactccct
    28801 gctgaaggag gtggggttat ccggtgaagg acttacagcc ctgacacgtc cactggtcat
    28861 gcctgccccg gaaaaggttc cggatgtcag cgaaccggta accgctccgg ataactcaga
    28921 accacaacag gtttaatccc cgtgccggaa tttttccggc atctttttca caaagaatgc
    28981 cctcacggga tattctggtt taaaagagga ttgaaaatgt ccgcagaaaa attcagagtt
    29041 gtcatataca ccaatacatg tgaaaagaaa gtcagttata ttcctgcaac agaagatata
    29101 aacatcgcta taatgccatt tcagacggca gacaatggct taattattgt gtgtcagaac
    29161 tcccgtataa tacgacgcaa agagcgtata cccggaagca gctgtgaatg gaaagatgtg
    29221 acattagcac caaaatccag aaaccgcgtt cagttatagc ctgacagcct gctttttatc
    29281 cctcagaaaa gggggataaa aattaaatat ttcacaaccg agatattttt caggaatgca
    29341 gattatttta tgttatgacc aacagagaaa caggcatgag aacaatcaca acgcgtgaac
    29401 aattattaat taatgggaga gtccacgaaa gaatcgccac acatatagtt acaggagctc
    29461 atgggtatga aacgctttgc acaaatggct ataacgtcag atacaacaag gcgcagcaac
    29521 tggttgaaag ctgcgaaaaa atagctgaag gcaaactacc tgttacctgt cctgtctgtt
    29581 tcgccatatg gcaggatgta cacaggttca cccacgttga ctttgacaca caatcgggga
    29641 aaagtgattt tattaatacc tgccattcgg agatcataac cggaaatgtt tcaataacag
    29701 gcaagaaata aaataatgct cccgttctaa aatgcaattc actcttataa tccccccacg
    29761 aacatgaacg ttcattatca ataacccgat gaaatgatat tcatctgaag gccagttacc
    29821 cgccacctgt cttataccgt aacccgtaat ataccggatt ttattctatg caaaaaaaat
    29881 cacttacact ccttctgccg tcattactca gcggctgtac ggcatttaat gccacgcctg
    29941 ttcctgacac cacacctccg gctctggtgt tcgtcgacgg acaaattcgc gaaagcgctg
    30001 ccattatcgc ccatacgcaa aaacgtctgc ttccgccacc ggtgacccgc cctgtgacgc
    30061 cgccccgccc gacaacaaca gcgacgccca caaccgcagt cagcagccag agtaccgcac
    30121 tgcctccccg cccgctcctt caggccacac agctgcccgc actggttatg accggtaaac
    30181 ccggtcagtc atccgtcgtt tatttacgtg aggcacgtca gcgctccgtc agtgactgga
    30241 tacgccagtt gctgccaccc ggctggcagt tccggcaaca gaacgctgcc ataccggcac
    30301 tgaacaaacg cctccccgtc tggtccgtta atgaccagtg gcaccgggca ctgaatactc
    30361 tgctgaccgg gcagcaactg tacggacata tggactggaa caggaaaata ctgactgtca
    30421 cgaccactgc agcaccgcct gttgacctca cggcaccgca gggttcacag aaaacagcgg
    30481 acacaccacg taatccgttc aggagcacaa ccggaacagc ggcagaccag gcacaggtac
    30541 cacctgccgt cagcgcttcc cgggttcaga gcacaacagg gacatccggt acatctgcgc
    30601 cccgcacaac cacgccagcc ccagtggtca cgaaaccggt agcgccacca ccacagaagg
    30661 tcacaccgcc acggcagtgg aaaatggaaa aagggaccac gctgaaggac ggactggccc
    30721 gctggacgtc gtccgaaaaa tgcccccacg gcacctggac gctgcgctgg gaaacgcccg
    30781 ttaactacag tatcgatgcc cctctggttt tcaccggcag tttccgccag gcgctggaac
    30841 agctcttcgc actctacacc ccggtggaaa aaccgctgta tgcccggacc agcactgcac
    30901 aatgtctgct gcgggtgaca gatcgtccac aggaataacc ttatgggctg gttcatcgtg
    30961 ttcatcatgg tcatcacgac cacaggcaat ttctggttca ccagtcagct ctcccgcagc
    31021 gaacaccgtc atcaggccgc agaaaacaca caacaggcag caacgttcat tcgttatatg
    31081 aacgccatca atgattacct gcatcagcat ccggaacgac ggacagccgg agggcggctg
    31141 acatccgctc agctcgggat tcccgggacg gataccgtca gtcatatcat cagccagcaa
    31201 cgtgtttttg tctgggctac agaaaccccg ggcctgatgg ccgcgctcag ggaacagagt
    31261 aatgactctg ctctgctcgg cagggtggaa aacggccgac tgctggacac agccggcaga
    31321 gcgctttccg tcacacttcc gtcagtgatc cccgatcacg tcatcctctg gatgaattag
    31381 tcacgaatct gaataaatca cggatctccc atgaataaaa aaattcttgc cattatgatt
    31441 gccgccgctc tgttacaggg ctgtgccagt tacagacagc ttgagagcac aagaaaggaa
    31501 gccgttgcgg aacaacaacg ggcacgggaa atgatgtctg atctgcgaac ccggacgccg
    31561 gttgttaagg aatcctcacg acaatggatc aatccccgtc cggtcatgga taaaaacacc
    31621 ggcaaaaccg ttcccggttg tcccattgtc attcataccc gtcagagcat cacccttcag
    31681 caggtggttc agcggatcac ggaaagctgc catattccgg tacggatcac acctgatgta
    31741 ctgacatacc tgaatacatc cacaaccggc agcacacaac aaatccaggg cgcattaccc
    31801 ccaccggatg cgagcggcat ggttcccctt gccgccattg gcgcatctga cacgccacca
    31861 tcccgtctgc cgggaaatac cgcagcactg tctgaactga atgcctccgg cctgacgacc
    31921 ctgctgaatg cggtctcctc ccgtctcggt atatcctggc gctatgacgg ctcgcatatc
    31981 atcttctatt atctcgagac acagaccttc cccatcacct atatggacag tcaggtggcc
    32041 tataacgcca ctgtggtcag cggcaccatg tcctcaaacg gctcatcagg aggcagtgca
    32101 tcagatgcca tttccggtga tgccagcaat acccagacca ccaccgtgga tatgaagtct
    32161 gcgctgtatc aggatgtgaa aaatgcggtc acatcaatgc tgaccccggg tatcggacgc
    32221 atgttcatgt ccacgggctt tatcaccgtc acagataatc cacaggttct ggaaacggtc
    32281 cgggcattca ttgaaaaacg caatgaagag atgaaacgtc aggtggtgct gaatgtggaa
    32341 attctgagta tccggaaaac ccgcaatgag caggccggta tcgactggaa cgcagtcttc
    32401 agcgacagaa cgctgggact gagtctcggc agcacattca ccagtgcagc cagtgatgcc
    32461 atcacaggtg gtgtttccat tgtggacggt aaactcaccg gttcaaaagc cttcctgaaa
    32521 gcattgtctt cccaggggga tgtcagtgtg gtgacccaga actctgccgt caccaaaaac
    32581 ctgacgccgg tgccaatgca gatcgccaat cagcaaagtt acatcgagag tgtgacgacg
    32641 gataccacag ccaatgtcgg ctcatccacc tctctgaatg cggccaccat caccaccggt
    32701 tttaacatga cccttctgcc gttcattctc ccggactcac agacactgca gttgctctat
    32761 tccatgagtc tctccgacaa accggttatt gagaattatg agagcggtgg aagtaaagcc
    32821 cagctgccga atgtggatct gaaaaccatc aatcagaccg tggatctgaa atccgggcag
    32881 acggtcatta tcagtggctt ccagcagtca ggacgtcgca gcggaaaaca gggtgtaggt
    32941 acgccgggat tcttcggtct tggcggtggg atcaacagtg aaaacgacga caccattctc
    33001 gtggtgctga tcacgcccaa tatcatctga tttgcacaag gaggacgatc gtgacggata
    33061 tttcaccgac agggcggcag gtgtttccgc tgacggcagg gcgcaatgtg tttctggccg
    33121 gtctggaatg gaaaacactg ccctcacagt accggcatgc ccgcgacttt gcccgggcac
    33181 agaaagccga ccttttcctg gcctgtcagt atctcagtaa tgaagatgcc gatacccaca
    33241 ccatggtagc cactgtcagt cgccgcatac tgcccggaaa accccgtcag tgcttctccc
    33301 tcgccctgct gatcctgcca ctgcttgaac atggcggcta tgccatcacg gaactgacgt
    33361 tacccggaga aacaccgcgc tatagctttg tttccgttgt cgacggtgtt ctcgtcagtg
    33421 acctggtggg aagcggggaa gaggttcggg aagcccggga tacctttctt tccatcaaca
    33481 ccgaaccaac tcagggctgg atacactatg caccggcggc attttcagca ggcgatcaga
    33541 atcagcacct gccgctggcc tccctgactg gctctggtaa acatccggct gccgcacgcc
    33601 tgaatccggt atcccgggga ccgcagttca tcactgtctc tctgatcatc gcccttctcg
    33661 gcactgcatg gtacggctgg cagtattatg aacgctggca gacagaaaaa gccgcgcagg
    33721 cagcggttgc ccgacaggcc acagaggaac gaatcactcc cccctggccc ggactccctg
    33781 aaacagacgc gttcatccgg ggatgtgctg atatatggac ttcactgcca ctgtctgctg
    33841 ccggatggcg ttattcgctg gcagaatgct cgactgacgg tggcagcggc aatcttcgcg
    33901 catcgtacac caatactgcg ggcgcgacag tgactgactt catccggcgc atcagtgata
    33961 tgacagacag ccgaccgttt attgtgatgc cggaaggaaa gaccggcggc attggcctgc
    34021 cggtcacctt ccggctgtct gaaaacgccg tcacagttga tgcactaccg gaaaccgctg
    34081 tcctgcagga acgactgacc tcgctggctc agtcaatgca actgaaactg gactggcagg
    34141 aagttaataa cagcctcact gatgagaacg gacagatcat tcagccccca tggaaagagt
    34201 atgaccttca gatgcagacc acactccctg cacaccatct ggctgaacgc ctgaaagagc
    34261 cttctgtccg ttttatctct gtcacacggc aactggagaa cggtcgtttc cactatcaat
    34321 ttacaggtaa atattatgcc cgataaagcc aaaacccttc tgttttcagt cctgctgtta
    34381 ccggccacgg caatggccac aaccggaaat gcagagcaac agaacacaca gcaaataagg
    34441 attgaacaga tacaggaaga gacagccctg tatgaagcta tgctggcgag agagaagatc
    34501 cgccatgaac tgcgaacact tgagcagggc gttcccgcag aggccggacg tcagcaggaa
    34561 agcatttccg cagttacaac aaatgtacag gcattaccat ccccccgcct gacagcaata
    34621 ttcggtgttc atcctcagct gactgcccgg atagaacttc cggggggaag catctccgat
    34681 gtgaaggcgg gccagaacat ccccggaacc acttatcacg tggaaaaaat cacgccggac
    34741 agtgtggtgc tggaacagtc tggcaccaga aaaaccctgc gaccatgaga ccggccgccg
    34801 taactggcgg ctgtcaaaaa taaaaggatg aaagacatat tttccgggca ccgctgcccg
    34861 taaggaggct ttatgctgga tccagcagac ctgccgccgg atgacattct ggattatgtg
    34921 gccatcgaca ccgataacgc cggacaactg cggatcaggg ttgttgaggg aaaaaaacat
    34981 ctgcgtgccg tccaggaata tctgacccgc cttcgcgcac gctatcaggg gcgtgtggga
    35041 gactttgagt tcaccacgct ggatgtgata gcccgcctca gacaggacgc cacaacgaca
    35101 ggcgatgaaa acgctattaa cccgatacag cagaaaatgc tcggctatat ccgccattct
    35161 gcctcccttg gtgcctccga ccttcatatg acgccagggc gggacaacac agacttcacc
    35221 tatgtggaag cccgggttca tggcgaactg gaagtgctcg atatcctccg gaaagaagaa
    35281 ggtctggaat tactgggggc tacctattcc ggcatgacgg atgtcatcaa gggcacacaa
    35341 tttgatcccg gggttccaca ggacgcacgt ctctctgaac agtacctgaa actggctggc
    35401 ctgtttggcg cacgctactc ccactacccc tgcgtgggag gcctctatgc tgttctccgt
    35461 ctgatcaagg atgacagcca gcaaatcccg accttcagta tgctgggtta tcacccggat
    35521 caggagtgca ccctgcgccg tatcctgcaa cgccctgaag gcattatcac cctctctggc
    35581 cccaccggct cgggaaaatc caccacgctg agaaccgcct ctgcggcata ccttgaacaa
    35641 tacggcttca acaacaccgg cggtattctt ctgccacggc gcaggttgtt cacgattgaa
    35701 tccccgccgg aaggccgtat ccccggagca attcagacag ccgttatgga taccacccgg
    35761 ggctgggtgg actccatcaa gtcagcactg cgtcttgatc ctgacagcat cctgaacggt
    35821 gaaatccgcg atcacgattc cgccattacg gcaataaaag ccgccatgac cgggcatctg
    35881 atgctgacca ccatccatgc aaacgacccg attaacatcc tcgaacgcct tgagatggag
    35941 ggtgttcagg ccaggatgat tgcagacccg cagttgttca tcggactgct cagtcagcgt
    36001 ctggttcagc tgatatgtcc ccactgtcgt catccatgga atgacgtagc atccagtcgt
    36061 acaaaggatg agcgacatct ggtggaaacc ttctgtaatg ctgatgcggt atatctgaga
    36121 aaccatgaag gctgtccaca ctgctggcgt ggtgtcacag ggcgtacagt gattgcagaa
    36181 gtcatctctc ctgacgccag attttttcag ttataccggg aaaaaggccg tatcgaagcc
    36241 aaaacctact ggcacaggga actgggcgga atgacgcgta atcagcatct gctgggaaaa
    36301 atcaacagcg gactggttga ccctctggcg gcccattaca tctctcccat tgatgaggac
    36361 agctatacgc tgctgcgctg atatgaaatt tctggaaaaa ctcaacagta ctgaactgga
    36421 tctgtctgcc ctgaacagct ggacagccag aaaaaccttc ggtcttaagc gtgccgagtt
    36481 ctatgagtcc ctcggcggaa tgatacgtga cggaacgccg tcactgcgcg ccctggagtt
    36541 tatctgtaat gtcgaaactg actttggcac ccgaaaagga acatccggca tgtactttct
    36601 ggccagagaa tgtattgcct ccctgaaagc cagcggtatg ctgtcggggg ccctgcccac
    36661 atgggttccc ccggaagaag cggcactgat caggaacggt gaagaacgcg ggaacatcgc
    36721 cgaagccctg ttccaggtcg caaaaaccat gaaagcacgg cgggaaatgg tgagcagtct
    36781 catcacactc tgtctttatc cccttctgct gctttcactc tgtgtggaga atatgtacaa
    36841 cgcacatacc cgcctcatcc ccattgtcag cgcttttgca ccggaggaga aatggtcatt
    36901 ccagatgaaa gtccttgcag ccatgtcaga gcttgtcatc ggctatggtc tgtggatcag
    36961 tggtgccatc atcacgcttg tcatcatcat ccgcttctca ctcagtcgct ataccggccc
    37021 gggacgcagg cttcttgaca aactgccacc atggtcgctc tatcagactt ttcatggtgt
    37081 gaattatctg ttcagcatat cgtcaatgct gcagataaat attccgcttt cacaggcgct
    37141 gctcagaatt gaaaaaagtg cagagcataa taaatggtta aaggagagaa ttcaggcaat
    37201 aagaaaaaat gtattatccg gtcagaatct ggccaccgcc atgcgcaatt ctgactatga
    37261 ttttccttca aaaatatgca tcaataaact tctcttaaat tctgaaaggg aagacagcgt
    37321 atctgcaatg aatacctatg ccggacagtg gctggaagat gcaaaaaata gcgtcagaaa
    37381 aagtggatta attatcaccg gtatttcagg aggttttgta ttctttttta ttcttaacat
    37441 gattagcgcg atatacagta tcagcgatat gctcaagcaa taatacctgc cgtcacacta
    37501 aaacgccctt caatatactt tcattaacgt aataaccatt aatacaggag tcatatatga
    37561 acttcagagc acgtctgtct tcttattttt ctttaaaaaa agataaagaa gatgataaag
    37621 gtttaacatt attagaaatt gccggcgcaa tgattatcat cgcaatagtt gtcgcgctcg
    37681 cgatgagcca gatgtccagc acaatggcct ccagtgacaa ccagcgcgaa ctgaaaaacc
    37741 tgcagacaat tgcaaccaaa atgaagcaat acaagttcca gggaagatat accggaacgg
    37801 attatatcaa aaccctgacg gaaagcggtg cattgcctgc tgatatgatt gcaggaggca
    37861 acaaagccaa aaacgcatgg ggtggtgatg tcacgattgc tgccacagca gacaaatatg
    37921 gctacattat caccagtaat aatgttccta aaacaaactg tgttgagctg attaacagcc
    37981 tgcgcagttc gtccatattt accaaaatta tgaacacagc cccgaacacg gtggatcccg
    38041 ttaccgtctg cagtagcgat aaaaacaata tcaagctgga aaccaacagc taatgccctg
    38101 ccacacagca ctccctccgg ggtgctgttt cccggagcac ataatcatga aatggtttta
    38161 tctctcatct cttttcttat tctgtctccc ggcgactgcc gccgtaaaca gtaacacact
    38221 ggatagctgc tttattcagg caggaaaacg ttactccatc gatcccggac tactgaaagt
    38281 aattgcccgt aaagaaagca gcctgaatcc ccgggccatc aatcataaca gaaatgccag
    38341 cggaaaaatt atcagcacag attatggcct gatgcagatt aattccacac atattcccgg
    38401 actgaaaaga atgggaatta tccgaagtga aaatgaactg ctcagtaatg tttgcctgaa
    38461 tatacagaca ggtgcatgga tccttgcccg gcattttcag cagtgtggtg tgaactggga
    38521 gtgtctcggt agctacaacg ccggatttaa taaaaataac actcaccgca gaatgaaata
    38581 tgcccggctg atttatgccg catatatgca gggacgttaa taacggagaa tacgagccat
    38641 gttcctcatc atcttactcg tgatctccgc tacgctgacc atacagttac ttgccatcag
    38701 agtggccgga cattatctca tgcaggtgca taccctccgg cttccacgtc acctctccct
    38761 gctctgtgtt gttctggcac tcaccgccac cattacgcac atcatccggg ggacagacat
    38821 taccggactt tgctttctgt tgctgatggc aggctgcatg acactgatga cactaactga
    38881 cgcaacctcc tgccgtcttc cccgtccctt caccctcaca ttcctgatgc tgggtgtggc
    38941 gtttcgttta tggcagggcg atgctgctat tgcgcttgcc tgtgctctgt tctggtttct
    39001 cctgttatat ctcctgaggc gctacacctc ataccgcaag ggagtggaga cgctgggact
    39061 gggggacgtg tttctgattg caggtgtggc catgtggtca tctgccaaca gcactccgtg
    39121 gatcatcggt attgccgcca ccggcaccat ggtctgtatg tcgctgttcc cgcgcagtaa
    39181 tacaggggcc gctatcccat ttggtccgtt tatctgtgca tcactgtaca ttttcacttt
    39241 ttttcctggt gccatttttc acaccggagg gtaacaccat gcaaaaagac gacgataaag
    39301 gttttgttct gtttgaaatt ctggccggac tgattgtgat cggtatcgcc acaccaatga
    39361 tttattcaga aattgaaaac tggctgaatg agcagcttta ccagagtgct gcatatcatg
    39421 cagatgccta caataccgca gccagaaact atattgccga caacaatgcc cgtctgcata
    39481 gcggcagcct tccggcaaat ttcacggctg atgatctgat tcgtcaggga tatctgaaac
    39541 aggggtttaa ccattctccg tttggtcaga gttatatcac cggtatccgc cgtaaccaga
    39601 ccaccgggcg tctggaagcc ctgacctgct caactggagg gcagacaatc aaagaagaag
    39661 ggctgcgtag tgtggcgggt cagttgccgg ggctgggtgg atttatcagc aaaaacggaa
    39721 cggcaacagg cgcattcgga gcctggacag ataaaccggg agactacgga cttacctgtt
    39781 cgacaggtca catcgccgtc gtcatgagcg gtgatgacct gcaggaaagt gaccgtctct
    39841 accgttttca ggtagcaggt cgtcctgaac tgaatcagat gcatactgca ataaatatgg
    39901 gcgggaataa cataaacaac accggcaata tcaatggtca gagtgccaca ctgaaaggtg
    39961 atattacgag tgaagacggc tggctaatca ccagaaacaa taagggatgg atgaatatca
    40021 cacatgatgg tggattcacc atgacggaca gccagtggat acgcgcggtt aatggcaaag
    40081 gcatcacaac caccggggaa atcaaagggg ggaaagtcag tggtggtaca gtccggtctg
    40141 acggtcgtct ttcaaccggg gagtatttac agcttgataa aacagctacg gcaggcacaa
    40201 agtgttcacc ggacggactt gtaggccgca ccagtacagg ggccatactt tcgtgccaat
    40261 ccggtatgtg ggcaggtttt gaatcctacc ctgtcggaag ccctataccg tggccatcag
    40321 ccacccctcc acaaggctac ctggtgatga acgggcagtc attttcatgt tcccggtatc
    40381 ctcagcttgc ccgtgcttat cccgggtgta agctgccgga tttgcgcggt gtttttatcc
    40441 gtggctggga caacggacgt gggctggatg gagatcggaa cagacatctc ctgagttatc
    40501 aggccgatca atccggtgtc tatcatgagc gtgggggatg gctgaaaggg catcacagcg
    40561 gtatgcctta ctgggcccag ggaagcacaa cggaaatgcg ccccaaaaac attgccttta
    40621 actacatcgt caaagcaagc tgatgcattt aaggcgtatt aacttttaac ttacagcaac
    40681 gtattttgac ccagaccatg cttcccgaat accccggttt acctctgtac gctctgaatc
    40741 ctgccgcacc atagcctgct ttgccgtcac agacggcaaa gccgtttgag ccactacccc
    40801 agttcgcata aaccggtttt ctggagtctt cttctgcgtc ataacaggcc gaataatcaa
    40861 aaaccagtgc atcgctgata ttattcttcc agacaccgga ttggcacgct ggctactgcc
    40921 tgacgcttaa ttaaataccg gccccaggta tattgagaca cgtaacagat atcgttccac
    40981 ctgactcata attaccccac gcataattat tgacatacca gacattattt ttatcggaat
    41041 aaacctgctg aaaaccaccg aaaggagaac cattaagata cgtccatgca caatatgcat
    41101 ggacaccaag tctgaaatta cgaatatttt ttccgatgtt ataggtctgc gttgtgaatt
    41161 taatttcagt tgaccgccag acaccggatt ggcacgaaag tattgcattt ctgtatccag
    41221 cctcctacct tccccgtttt tatcgtggaa ggcagatatg tttcaaaaaa acaaaactcg
    41281 caaaatgtcc ctcagccggg cactggacaa atacctgaag actgtttccg tccataaaaa
    41341 aggtcatctg caggagtttt atcgcgtcaa tgtgattaaa cgtcacccta tggccgatcg
    41401 ctatatggat gaaatcacta ccattgatat cgccagttac cgggatcagc gtttagctca
    41461 aattaaccca cgaacagggc gtcaaatcac cggaaatacc gtccgtcttg aacttgccct
    41521 tttatcatca ttattcaata tcgcccgtgt ggaatgggga acatgccgta tgaatcctgt
    41581 tgaactggtc cgaaaaccga aaatcagtaa tggccgcgac cgccgactga cttctggtga
    41641 agaacgccgc ctttcccgct atttcaggga caaaaatccg cagctctacg ttatcttcca
    41701 tctggctctg gaaacagcaa tgcgccaggg agaaattctc tcattacgct gggaacacct
    41761 cgatttgcag cacggcgtgg cgcatcttcc tgaaacgaaa aatggctcac cacgcgatgt
    41821 cccactctcc cggaaagcac gtaactacct gcaaattctg ccacaacaga taaacggcaa
    41881 tgtgttcagt tacacctcgt ccggtttcaa aagcgcctgg cgtaccactc ttctggatct
    41941 gaaaatagaa aatctgcatt ttcacgatct ccgccatgag gccatcagcc gtttgttcga
    42001 gctcggcaca ctgaacgtta tggagattgc agccatttcc ggtcaccgct ccctgaacat
    42061 gttgaaacgg tacacacatc tccgggcata tcacctggtc agtaaactcg atgcaaggcg
    42121 taaacagacc agcaaaattt ccccttattt tgttccctac cctgcaactg tcagaaacag
    42181 aaacggagtg ttcattgtga cactgcacga tttcgacctt gaaagccgtg ctgaaacccg
    42241 ggaactggca atatctcacg ccagcgtatt gttgctgcgc gcactggccc aggctgctca
    42301 gcggggtgaa cgcgtcccca caccaggcga gctacccgca aacattgatg aacgggtcat
    42361 gatttgtccg ttaacaaaca gccagttcat gacaaaagcg aacagtattt tcatatcctc
    42421 ctcctgaaca aataattttt gttcaaaatt aacgctatga gaccctttat gaaacactta
    42481 tcctcccgtg cagccactgc ggataataat cgtatcgtaa caattgaaga cgtttacaca
    42541 atcgcattct cgcaaccaat tcacaccgaa tcagcgttgg ggcaaaggtg gatgttgatc
    42601 catactggtg aatatgaaag tgccagcgat gcaaaacgct tccttgatac gctcgtcacc
    42661 gggcactgtg cttgcatggt aacaccatca cctgttctgg atcatgacac ctggctgcgt
    42721 atccgcctgc tgatgggcga gataagggat gccaaataaa cattcatggc cccaacacat
    42781 aaaaataacg ccggggactc tggcagaggt accattcttt ttacatagag ccaatatata
    42841 aacaactttt ctatgataat atctttatca tgttataaat aattgccccc tcagcagaaa
    42901 gggcccaaat agaaaaagca aaaatc
//
LOCUS       DAZFQD010000025        42493 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.025, whole genome shotgun sequence.
ACCESSION   DAZFQD010000025 DAZFQD010000000
VERSION     DAZFQD010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 42493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42493
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.025"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            215..910
                     /gene="aqpZ"
                     /locus_tag="ACHKTW_001442"
     CDS             215..910
                     /gene="aqpZ"
                     /locus_tag="ACHKTW_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015250 - water channel activity [Evidence
                     IEA];
                     GO_process: GO:0006833 - water transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HFO6462495.1"
                     /translation="MFRKLAAECFGTFWLVFGGCGSAVLAAGFSELGIGFAGVALAFG
                     LTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLI
                     ASGKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGATDKFAPAG
                     FAPIAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGII
                     GGLIYRTLLEKRD"
     gene            1405..2304
                     /gene="lysO"
                     /locus_tag="ACHKTW_001443"
     CDS             1405..2304
                     /gene="lysO"
                     /locus_tag="ACHKTW_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HFO6462496.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQAALKVINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHTTEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVVSSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
     gene            2448..4100
                     /gene="hcp"
                     /locus_tag="ACHKTW_001444"
                     /gene_synonym="priS"
     CDS             2448..4100
                     /gene="hcp"
                     /locus_tag="ACHKTW_001444"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HFO6462497.1"
                     /translation="MFCVQCEQTIRTPAGNGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKTREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGEDFSAVIAQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLSA"
     gene            4112..5080
                     /gene="hcr"
                     /locus_tag="ACHKTW_001445"
     CDS             4112..5080
                     /gene="hcr"
                     /locus_tag="ACHKTW_001445"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HFO6462498.1"
                     /translation="MTMPTNQCPWRMQVHHITQETPDVWTISLICHDYYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLAGVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            5213..6931
                     /gene="poxB"
                     /locus_tag="ACHKTW_001446"
     CDS             5213..6931
                     /gene="poxB"
                     /locus_tag="ACHKTW_001446"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0052737 - pyruvate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HFO6462499.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDGLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNHVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKADRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIVVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            6968..7969
                     /gene="ltaE"
                     /locus_tag="ACHKTW_001447"
     CDS             6968..7969
                     /gene="ltaE"
                     /locus_tag="ACHKTW_001447"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HFO6462500.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRERN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMAGGGMRQSGILAAAGMYALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            7980..9410
                     /gene="ybjT"
                     /locus_tag="ACHKTW_001448"
     CDS             7980..9410
                     /gene="ybjT"
                     /locus_tag="ACHKTW_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HFO6462501.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQVALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPASEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTAL
                     RALIPQRLIAFDDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLAALWQVVNQIGGKERYFFGNILWQTRALMDRAIGHKLAKGRPEREYLQTGDAVD
                     SWKVIVVEPEKQLTLLFGMKAPGLGRLCFSLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKQIARLAEQSTD"
     gene            9509..10522
                     /gene="ybjS"
                     /locus_tag="ACHKTW_001449"
     CDS             9509..10522
                     /gene="ybjS"
                     /locus_tag="ACHKTW_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFO6462502.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRATGRNEAMGKLLEKMG
                     AEFVPADLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRDIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPVITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(10519..11349)
                     /gene="amiD"
                     /locus_tag="ACHKTW_001450"
     CDS             complement(10519..11349)
                     /gene="amiD"
                     /locus_tag="ACHKTW_001450"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HFO6462503.1"
                     /translation="MRRVFWLIAAALLLAGCAGEKGIVEKEGYQLDTRRQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLVPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSAGVKYFAPFEPAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(11346..11669)
                     /gene="ybjQ"
                     /locus_tag="ACHKTW_001451"
     CDS             complement(11346..11669)
                     /gene="ybjQ"
                     /locus_tag="ACHKTW_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HFO6462504.1"
                     /translation="MQFSTTPTLEGQTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            11795..12310
                     /gene="ybjP"
                     /locus_tag="ACHKTW_001452"
     CDS             11795..12310
                     /gene="ybjP"
                     /locus_tag="ACHKTW_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFO6462505.1"
                     /translation="MRYSKLTMLIPCALLLSACTTVTPAYKDNGTRSGPCVEGGPDNV
                     AQQFYDYRILHRSNDITALRPYLSDKLATLLSDASRDNNHRELLTNDPFSSRTTLPDS
                     AHVASASTIPNRDARNIPLRVDLKQGDQSWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            12528..13256
                     /gene="artP"
                     /locus_tag="ACHKTW_001453"
     CDS             12528..13256
                     /gene="artP"
                     /locus_tag="ACHKTW_001453"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HFO6462506.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            13274..14005
                     /gene="artI"
                     /locus_tag="ACHKTW_001454"
     CDS             13274..14005
                     /gene="artI"
                     /locus_tag="ACHKTW_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtI"
                     /protein_id="HFO6462507.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEASYPPFESIDANNQIV
                     GFDVDLAQALCKEIDSTCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDGVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            14012..14728
                     /gene="artQ"
                     /locus_tag="ACHKTW_001455"
     CDS             14012..14728
                     /gene="artQ"
                     /locus_tag="ACHKTW_001455"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HFO6462508.1"
                     /translation="MNEFFSLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            14728..15396
                     /gene="artM"
                     /locus_tag="ACHKTW_001456"
     CDS             14728..15396
                     /gene="artM"
                     /locus_tag="ACHKTW_001456"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HFO6462509.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            15688..16419
                     /gene="artJ"
                     /locus_tag="ACHKTW_001457"
     CDS             15688..16419
                     /gene="artJ"
                     /locus_tag="ACHKTW_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtJ"
                     /protein_id="HFO6462510.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPERSKQVSFTT
                     PYYENSAVVIAKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(16594..17721)
                     /gene="rlmC"
                     /locus_tag="ACHKTW_001458"
     CDS             complement(16594..17721)
                     /gene="rlmC"
                     /locus_tag="ACHKTW_001458"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HFO6462511.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKPLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSETKLAQLRKALPWLQE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIASEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVLTLLVKQ"
     gene            complement(17762..18250)
                     /gene="ybjO"
                     /locus_tag="ACHKTW_001459"
                     /pseudo
     CDS             complement(17762..18250)
                     /gene="ybjO"
                     /locus_tag="ACHKTW_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
     gene            complement(18310..19155)
                     /gene="potI"
                     /locus_tag="ACHKTW_001460"
     CDS             complement(18310..19155)
                     /gene="potI"
                     /locus_tag="ACHKTW_001460"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HFO6462512.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDDAMMSAVGLSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARHG"
     gene            complement(19152..20105)
                     /gene="potH"
                     /locus_tag="ACHKTW_001461"
     CDS             complement(19152..20105)
                     /gene="potH"
                     /locus_tag="ACHKTW_001461"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HFO6462513.1"
                     /translation="MNTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALPYIWLILLF
                     LLPFLIVFKISLAEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDTYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            complement(20115..21248)
                     /gene="potG"
                     /locus_tag="ACHKTW_001462"
     CDS             complement(20115..21248)
                     /gene="potG"
                     /locus_tag="ACHKTW_001462"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015417 - ABC-type polyamine transporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HFO6462514.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEIATRVNEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLDSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            complement(21343..22455)
                     /gene="potF"
                     /locus_tag="ACHKTW_001463"
     CDS             complement(21343..22455)
                     /gene="potF"
                     /locus_tag="ACHKTW_001463"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HFO6462515.1"
                     /translation="MTALNKKWLSGLVAGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     NLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYANANKAATPLVSAEVRDNPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            complement(22806..23282)
                     /gene="ybjN"
                     /locus_tag="ACHKTW_001464"
     CDS             complement(22806..23282)
                     /gene="ybjN"
                     /locus_tag="ACHKTW_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HFO6462516.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            complement(23370..24272)
                     /gene="rimK"
                     /locus_tag="ACHKTW_001465"
     CDS             complement(23370..24272)
                     /gene="rimK"
                     /locus_tag="ACHKTW_001465"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HFO6462517.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGDFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            complement(24333..25055)
                     /gene="nfsA"
                     /locus_tag="ACHKTW_001466"
     CDS             complement(24333..25055)
                     /gene="nfsA"
                     /locus_tag="ACHKTW_001466"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HFO6462518.1"
                     /translation="MTPTIELICGHRSIRHFTDEPISDAQCEAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALIAAESLGLGGVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLGWP
                     AENPDLKPRLPASILVHENSYQPLDKGALAQYDEQLAEYYLTRGSNNRRDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            complement(25039..25326)
                     /gene="ybjC"
                     /locus_tag="ACHKTW_001467"
     CDS             complement(25039..25326)
                     /gene="ybjC"
                     /locus_tag="ACHKTW_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HFO6462519.1"
                     /translation="MRAIGKLPKGVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNRPPQYSRSEREKDNDANH"
     gene            25486..25743
                     /gene="grxA"
                     /locus_tag="ACHKTW_001468"
     CDS             25486..25743
                     /gene="grxA"
                     /locus_tag="ACHKTW_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HFO6462520.1"
                     /translation="MQTVIFGRSGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            complement(25773..26150)
                     /gene="ybjM"
                     /locus_tag="ACHKTW_001469"
     CDS             complement(25773..26150)
                     /gene="ybjM"
                     /locus_tag="ACHKTW_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HFO6462521.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKKQ"
     gene            26420..28105
                     /gene="ybjL"
                     /locus_tag="ACHKTW_001470"
     CDS             26420..28105
                     /gene="ybjL"
                     /locus_tag="ACHKTW_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HFO6462522.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLRHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            complement(28280..28816)
                     /gene="rcdA"
                     /locus_tag="ACHKTW_001471"
     CDS             complement(28280..28816)
                     /gene="rcdA"
                     /locus_tag="ACHKTW_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HFO6462523.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            28900..30108
                     /gene="ybjJ"
                     /locus_tag="ACHKTW_001472"
     CDS             28900..30108
                     /gene="ybjJ"
                     /locus_tag="ACHKTW_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO6462524.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGVPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     RYSRVAVVRASALMGALGIGLIIFIDSAWVAGVSVVLWGLGASLGFPLTISATSDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTQTAMENS"
     gene            30108..30923
                     /gene="ybjI"
                     /locus_tag="ACHKTW_001473"
     CDS             30108..30923
                     /gene="ybjI"
                     /locus_tag="ACHKTW_001473"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HFO6462525.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVVSEGKDVFNGELSKDAFTTVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGSAVVAAAKYRAGSNNREGVLDVIDKVLKHEAPFDQ"
     gene            31009..31293
                     /gene="ybjH"
                     /locus_tag="ACHKTW_001474"
     CDS             31009..31293
                     /gene="ybjH"
                     /locus_tag="ACHKTW_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbjH"
                     /protein_id="HFO6462526.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYVRHLASVGEGMVIEHQIDDHHH"
     gene            complement(31334..32566)
                     /gene="mdfA"
                     /locus_tag="ACHKTW_001475"
     CDS             complement(31334..32566)
                     /gene="mdfA"
                     /locus_tag="ACHKTW_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
                     /protein_id="HFO6462527.1"
                     /translation="MQNKLASGARLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVV
                     EQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLAGVVWFIITCLAILLA
                     QNIEQFTLLRFLQGISLCFIGAVGYAAIQESFEEAVCIKITALMANVALIAPLLGPLV
                     GAAWIHVLPWEGMFVLFAALAAISFFGLQRAMPETATRIGEKLSLKELGRDYKLVLKN
                     GRFVAGALALGFVSLPLLAWIAQSPIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLA
                     RLTSRRTVRSLIIMGGWPIMIGLLVAAAATVISSHAYLWMTAGLSIYAFGIGLANAGL
                     VRLTLFASDMSKGTVSAAMGMLQMLIFTVGIEISKHAWLNGGNGQFNLFNLVNGILWL
                     SLMVIFLKDKQMGNSHEG"
     gene            32851..33447
                     /gene="ybjG"
                     /locus_tag="ACHKTW_001476"
     CDS             32851..33447
                     /gene="ybjG"
                     /locus_tag="ACHKTW_001476"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFO6462528.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGFTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRVCFALPIRKGWVRD"
     gene            33505..34263
                     /gene="deoR"
                     /locus_tag="ACHKTW_001477"
     CDS             33505..34263
                     /gene="deoR"
                     /locus_tag="ACHKTW_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HFO6462529.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTQRIKLMY"
     gene            complement(34310..35512)
                     /gene="dacC"
                     /locus_tag="ACHKTW_001478"
     CDS             complement(34310..35512)
                     /gene="dacC"
                     /locus_tag="ACHKTW_001478"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HFO6462530.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLGAKTDRIRFNESEK
                     LLTWGFRFFETVTPIKPDATFVTQRVWFGDKSEVNLGAGEAGSVTIPRGQLKNLKASY
                     TLTEPQLTAPLKKGQVVGTIDFQLNGKSIEQRPLIVMENVEEGGFFGRMWDFVMMKFH
                     QWFGSWFS"
     gene            35759..36385
                     /gene="gstB"
                     /locus_tag="ACHKTW_001479"
     CDS             35759..36385
                     /gene="gstB"
                     /locus_tag="ACHKTW_001479"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
                     /protein_id="HFO6462531.1"
                     /translation="MITLWGRNNSTNVKKVLLTLEELELPYEQILAGREFGINHDADF
                     LAMNPNGLVPLLRDDESDLILWESNAIVRYLAAQYGQKRLWIDSPARRAEAEKWMDWA
                     NQTLSNAHRGILMGLVRTPPEERDQAAIDASCKECDALFALLDAELAKVKWFSGDEFG
                     VGDIAIAPFIYNLFNVGLTWTPRPNLQRWYQQLTERPAVRKVVMIPVS"
     gene            complement(36382..37497)
                     /gene="yliI"
                     /locus_tag="ACHKTW_001480"
     CDS             complement(36382..37497)
                     /gene="yliI"
                     /locus_tag="ACHKTW_001480"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
                     /protein_id="HFO6462532.1"
                     /translation="MHRQSFFLVPLICLSSALWAAPATVNVEVLQDKLDHPWALAFLP
                     DNHGMLITLRGGELRHWQAGKGLSAPLSGVPDVWAHGQGGLLDVVLAPDFAQSRRIWL
                     SYSEVGDDGKAGTAVGYGRLSDDLSKVTDFRTVFRQMPKLSTGNHFGGRLVFDGKGYL
                     FVALGENNQRPTAQDLDKLQGKLVRLTDQGEIPDDNPFIKESGARAEIWSYGIRNPQG
                     MAMNPWSNALWLNEHGPRGGDEINIPQKGKNYGWPLATWGINYSGFKIPEAKGEIVAG
                     TEQPVFYWKDSPAVSGMAFYNSDKFPQWQQKLFIGALKDKDVIVMSVNGDKVTEDGRI
                     LTDRGQRIRDVRTGPDGYLYVLTDESSGELLKVSPRN"
     gene            complement(37608..37991)
                     /gene="bssR"
                     /locus_tag="ACHKTW_001481"
     CDS             complement(37608..37991)
                     /gene="bssR"
                     /locus_tag="ACHKTW_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HFO6462533.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            38204..39529
                     /gene="rimO"
                     /locus_tag="ACHKTW_001482"
     CDS             38204..39529
                     /gene="rimO"
                     /locus_tag="ACHKTW_001482"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HFO6462534.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ADMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            complement(39576..40904)
                     /gene="dgcI"
                     /locus_tag="ACHKTW_001483"
     CDS             complement(39576..40904)
                     /gene="dgcI"
                     /locus_tag="ACHKTW_001483"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFO6462535.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYSLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQVVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLLGLE
                     RFSLLSKSLAMDSENLMFSLFKNGKPVTGDEYNAKNAIFTVSEAMEHFAYLPTGLYVF
                     AYKKDVYLRVCTLIIFFAALVAVISGTSCLYLVRRVINRGIVEKEAIINNHFERVLDG
                     GLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGGYLCLFDVDKFKNI
                     NDTFGHLLGDEVLMKVVKILKSQIPVDKGKIYRFGGDEFAVIYTGGTLEELLSILKEI
                     VHFQVGSINLSTSIGVAHSNECTTVERLKMLADERLYKSKKNGRAQISWQ"
     gene            complement(40912..>42493)
                     /locus_tag="ACHKTW_001484"
     CDS             complement(40912..>42493)
                     /locus_tag="ACHKTW_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001400151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cyclic diguanylate phosphodiesterase"
                     /protein_id="HFO6462536.1"
                     /translation="AKTSAVPNNALFEIYFYYPGGNLLNASDKLFYLPFAFIIIVLLV
                     VYFMTTRVFRRQFSEMTELVNTLAFLPDSTDQIEALKIREGDAKEIISIKNSIAEMKD
                     AEIERSNKLLSLISYDQESGFIKNMAIIESNNNQYLAVGIIKLCGLEAVEAVFGVDER
                     NKIVRKLCQRIAEKYAQCCDIVTFNADLYLLLCRENVQTFTRKIAMVNDFDSSFGYRN
                     LRIHKSAICEPLQGENAWSYAEKLKLAISSIRDHMFSEFIFCDDAKLNEIEENIWIAR
                     NIRHAMEIGELFLVYQPIVDINTRAILGAEALCRWVSAERGIISPLKFITIAEDIGFI
                     NELGYQIIKTAMGEFRHFSQRASLKDDFLLHINVSPWQLNEPHFHERFTTIMKENGLK
                     ANSLCVEITETVIERINEHFYLNIEQLRKQGVRISIDDFGTGLSNLKRFYEINPDSIK
                     VDSQFTGDIFGTAGKIVRIIFDLARYNRIPVIAEGVESEDVARELIKLGCVQAQGYLY
                     QKPMPFSAWDKSGKLVKE"
BASE COUNT        10345 a        11044 c        10632 g        10472 t
ORIGIN      
        1 aaatcttttg cttgcactac actgtcaatc agacaaatga ctatatgggg caacccgctg
       61 ttgccctaaa tcaaatttac tggatttatt tatctggtga atgactcgcc ttcgttttta
      121 aaatagccgc gcattatttc cgtcgtcact tttttacgac gaccctataa aacgaccata
      181 tttttcacag ggtcaatttt taattgaggt ggatatgttc agaaaattag cagctgaatg
      241 ttttggtact ttctggcttg tttttggtgg ctgtggtagt gctgtactgg ccgcaggctt
      301 ctcggaatta ggcattggtt ttgccggcgt ggcgttggcg ttcggtctga ccgttctgac
      361 gatggccttt gctgttggtc atatttctgg tggtcatttt aacccggcgg tcactattgg
      421 tttatgggct ggcggacgtt ttccggcaaa agaagtcgtt ggctacgtaa ttgcccaggt
      481 tgtcggcggt attgttgcag cggcgctgct gtatttaatt gccagtggta aaacgggttt
      541 tgacgcggca gccagcggtt ttgcttctaa cggttatggc gagcattcac caggcggtta
      601 ttccatgctt tccgcgctgg tagttgaact ggtattgagt gcaggtttcc tgttggtgat
      661 ccacggcgca accgacaaat tcgcgccggc aggttttgcg ccgatcgcta ttggtctggc
      721 cttaaccctg attcacttaa ttagtattcc ggtgactaac acttctgtta acccggcgcg
      781 cagcaccgcg gttgctatct tccagggcgg ctgggcatta gaacaactgt ggttcttctg
      841 ggtggtgcca attgtcggcg gcattatcgg tggtctgatt taccggaccc tgctggaaaa
      901 gcgtgattaa ttaacgcaga aggcctggcg taatgtcagg ccttttttta agatgttgtt
      961 atatgttgat tcatcatctt gttgcgctcc tttcatgagc taagccacat attgccactg
     1021 gcgcaaggag cgcgcagggg gcggccaatc gccgccgccc catgcacccc cgggctctgg
     1081 cgaacaaaat cgccgctgcg cggtgccctc gacttatccc ttacggctac cgggtcgggc
     1141 gcgaggtaac atccctgtaa aacgcgccct cagcccacat ccatgtgggc tgccccggcc
     1201 ttcagggaac gcctcggcga ttttgacgcc accaacatcg gtgcagtttg taattaaagc
     1261 gcaagaatat ctttaaatga aaacgaatac tctactgctc cttatgcata acatttcctt
     1321 ttaattgcaa ctttactcgt ccttccgctt tcagtagtgt gtcatagcgc atttcgactt
     1381 catctttgca aggactgggt tttcatgttt tctgggctgt taatcattct ggttcccctg
     1441 attgtgggtt acctcattcc gcttcgccaa caagctgcgt taaaagttat taatcagcta
     1501 ttaagctgga tggtttacct tattctcttt tttatgggta tcagtctggc gtttctcgat
     1561 aacctcgcca gtaacctgtt ggcgattctg cattattctg ccgtcagtat taccgttatt
     1621 ttactgtgta atattgccgc cctgatgtgg ctggagcgag gcctgccgtg gcgcaaccac
     1681 catcagcaag aaaaactccc gtcgcgtatt gcgatggcgc tggagtcgct aaaactgtgc
     1741 ggcgtagtag tgattggttt tgccattggt ctgagtggac tggctttctt acaacacacg
     1801 accgaagcca gtgaatacac gttaattttg ctacttttcc tcgttggtat tcagttgcgc
     1861 aataatggca tgaccttaaa gcagattgtc cttaatcgcc ggggaatgat tgtcgccgtg
     1921 gtggtggttg tcagttcatt aattggtggt ttaattaacg cctttattct tgatctcccc
     1981 atcaataccg cgctggcaat ggcctccggt ttcggctggt attctctttc cggtatttta
     2041 ttgaccgaat cttttggtcc ggtaatcggg agcgcggcgt tttttaatga tctggcccgt
     2101 gaactgattg ctattatgtt gatccctggg ctgattcgcc gcagccgctc tactgcactg
     2161 ggcttatgcg gtgccacatc aatggatttc accctgcccg ttcttcaacg tactggcggg
     2221 ctggatatgg tcccggcggc aattgttcac ggttttattc ttagcctgtt agtgccgatc
     2281 ctcatcgcct ttttctctgc gtaatacctc tctggcggta gatccctgcc gccaaaattg
     2341 cgctaaatca atctccctta aagttgcatg aaaaatccct tttatccccg cgttaagcgt
     2401 cttaacctta aacatgtata ttaaatataa ctttaaaagg tgtgatcatg ttttgtgtgc
     2461 aatgtgaaca aactatccgt actccggcag gaaacggctg ctcatacgcg caggggatgt
     2521 gtggtaaaac ggcggaaact tctgaccttc aggatttact catcgcggcg ctgcaagggc
     2581 tttctgcctg ggcggtaaaa acgcgtgaat acggcatcat caaccacgat gttgacagct
     2641 tcgcgccacg tgcctttttc tcaaccctga ccaacgttaa cttcgattct ccgcgtattg
     2701 tcggctacgc tcgtgaagcg attgccctgc gcgaggcgct gaaagcgcaa tgcctggctg
     2761 tagatgccaa cgcgcgcgtc gataacccga tggctgacct gcaactggtg agcgacgatc
     2821 tcggcgagct gcaacgtcag gcagcagaat ttacccctaa caaagataaa gcggcgattg
     2881 gcgaaaacat tctcggcctg cgtctgctgt gcctgtatgg cctgaaaggt gcggcggcct
     2941 atatggaaca cgcgcatgtt ctcggtcaat acgacaacga tatttatgcc cagtaccata
     3001 aaatcatggc gtggctgggg acctggcctg ccgatatgaa cgcgcttctt gagtgttcaa
     3061 tggaaatcgg ccagatgaac ttcaaagtga tgagcattct ggatgcaggc gaaaccggta
     3121 aatacggtca cccgacgcca acccaggtca acgtcaaagc gacggcgggt aaatgcattc
     3181 tgatttccgg tcacgatctc aaagatctct acaacctgct ggaacagacc gaaggcacgg
     3241 gcgttaatgt ctacacccac ggcgaaatgc tgcctgcgca tggctacccg gagctgcgta
     3301 aattcaagca tctggtcggt aactacggca gcggctggca gaatcagcaa gtagagttcg
     3361 ctcgtttccc tggccccatc gtgatgacct cgaactgcat catcgaccca accgtaggcg
     3421 cttatgacga tcgtatctgg acccgcagca tcgttggctg gcctggcgtg cgtcatctgg
     3481 atggcgaaga tttctctgcg gtcatcgccc aggcgcaaca aatggcgggc ttcccgtaca
     3541 gcgaaattcc tcacctgatc accgtgggct ttggtcgcca gacgctgctt ggtgcagccg
     3601 atacgctgat tgatctggtt agccgtgaaa aactgcgtca tatcttcctg cttggtggct
     3661 gtgacggcgc acgcggcgag cgccactact tcaccgattt cgccaccagc gtgccggatg
     3721 actgcctgat cctgaccctc gcctgtggta aatatcgctt taacaaactt gagtttggcg
     3781 atatcgaagg tctgccgcgt ctggtagatg caggtcaatg taacgatgct tactcagcaa
     3841 ttattctggc tgtcactctg gcagagaaac tgggctgtgg tgtgaacgat ctgccgctgt
     3901 cgctggtgct ctcctggttt gaacagaaag cgatcgtcat tctgctgacg ctgctttctc
     3961 tgggcgtgaa aaatatcgtc accggcccga ctgcacctgg tttcctgaca ccagacctgc
     4021 tggcggtgct gaacgagaaa ttcggcctgc gttctatcac cactgttgaa gaagacatga
     4081 agcaactgtt gagcgcgtaa ggagtttgtc gatgacgatg ccaacgaatc aatgcccgtg
     4141 gcggatgcag gttcatcaca ttacgcaaga aacgccggat gtgtggacga tttccctgat
     4201 ttgccacgat tactacccat atcgcgccgg gcaatatgca ctggtcagcg tgcgaaactc
     4261 agcggaaacg ctgcgtgctt acaccatttc ctccacgcca ggcgtgagtg aatatatcac
     4321 cctgaccgtg cggcggattg atgacggtgt cggctcccag tggctgacgc gcgatgtaaa
     4381 acgcggtgat tatctctggc tttcggacgc gatgggggaa tttacctgcg acgataaagc
     4441 agaagataaa ttcctgttgc tggcggcagg ctgcggcgtc acgccgatta tgtcgatgcg
     4501 tcgctggctt gcgaagaacc gtccacaggc cgatgtgcgg gtgatctaca acgtgcgtac
     4561 gccgcaggat gttattttcg ccgatgagtg gcgtaactat ccggtaacgc tggtggcaga
     4621 aaataacgtt accgaaggct ttatcgctgg tcgtctcact cgcgaactgc tggcaggtgt
     4681 acctgactta gcttcacgta ccgtgatgac ctgcggcccg gctccgtata tggattgggt
     4741 agagcaggaa gtgaaagcgc tcggcgtgac gcgtttcttt aaagagaaat tcttcacccc
     4801 agtagcagaa gcggcgacca gcggtctgaa attcaccaaa ctgcaaccgg cacgagaatt
     4861 ttacgccccg gttggcacca cgctactgga ggcgctggaa agcaataacg ttccggttgt
     4921 cgctgcctgc cgtgcgggtg tttgcggctg ctgtaagaca aaagtggttt ccggtgaata
     4981 tacggtgagc agtacaatga cgctgaccga cgccgaaatc gctgaaggtt acgtactggc
     5041 ctgctcctgc catccgcagg gggatttggt tctcgcataa tcgccttatg cccgatgata
     5101 ttcctttcat cgggctattt aaccgttagt gcctcctttc tctcccatcc cttccccctc
     5161 cgtcagatga actaaacttg ttaccgttat cacattcagg agatggagaa ccatgaaaca
     5221 aacggttgca gcttatatcg ccaaaacact cgaatcggca ggggtgaaac gcatctgggg
     5281 agtcacaggc gactctctga acggtcttag tgacggtctt aatcgcatgg gcaccatcga
     5341 gtggatgtcc acccgccacg aagaagtggc ggcctttgcc gctggcgctg aagcacaact
     5401 tagcggagaa ctggcggtct gcgccggatc gtgcggcccc ggcaacctgc acttaatcaa
     5461 cggcctgttc gattgccacc gcaatcacgt tccggtactg gcgattgccg ctcatattcc
     5521 ctccagcgaa attggcagcg gctatttcca ggaaacccac ccacaagagc tattccgcga
     5581 atgtagtcac tattgcgagc tggtttccag cccggagcag atcccacaag tactggcgat
     5641 tgccatgcgc aaagcggtgc ttaaccgtgg cgtttcggtt gtcgtgttac caggcgacgt
     5701 ggcgttaaaa cctgcgccag aaggggcaac cacgcactgg tatcatgcgc cacaaccagt
     5761 cgtgacgccg gaagaagaag agttacgcaa actggcgcaa ctgctgcgtt attccagcaa
     5821 tatcgccctg atgtgtggca gcggctgcgc gggggcgcat aaagagttag ttgagtttgc
     5881 cgggaaaatt aaagcgccta ttgttcatgc cctgcgcggt aaagaacatg tcgaatacga
     5941 taatccgtat gatgttggaa tgaccgggtt aatcggcttc tcgtcaggtt tccataccat
     6001 gatgaacgcc gacacgttag tgctactcgg cacgcaattt ccctaccgcg ccttctaccc
     6061 gaccgatgcc aaaatcattc agattgatat caacccagcc agcatcggcg ctcacagcaa
     6121 ggtggatatg gcactggtcg gcgatatcaa gtcgactctg cgtgcattgc ttccattggt
     6181 ggaagaaaaa gccgatcgca agtttctgga taaagcgctg gaagattacc gcgacgcccg
     6241 caaagggctg gacgatttag ctaaaccgag cgagaaagcc attcacccgc aatatctggc
     6301 gcagcaaatt agtcattttg ccgccgatga cgctattttc acctgtgacg ttggtacgcc
     6361 aacggtgtgg gcggcacgtt atctaaaaat gaacggcaag cgtcgcctgt taggttcgtt
     6421 taaccacggt tcgatggcta acgccatgcc gcaggcgctg ggtgcgcagg cgacagagcc
     6481 agaacgtcag gtggtcgcca tgtgcggcga tggcggtttt agcatgttga tgggcgattt
     6541 cctctcagta gtgcagatga aactgccagt gaaaattgtc gtctttaaca acagcgtgct
     6601 gggctttgtg gcgatggaga tgaaagctgg tggctatttg actgacggca ccgaactaca
     6661 cgacacaaac tttgcccgca ttgccgaagc gtgcggcatt acgggtatcc gtgtagaaaa
     6721 agcgtctgaa gttgatgaag ccctgcaacg cgccttctct atcgacggtc cggtgttggt
     6781 ggatgtggtg gtcgccaaag aagaattagc cattccaccg cagatcaaac tcgaacaggc
     6841 caaaggtttt agcctgtata tgttgcgcgc aattatcagc ggacgcggcg atgaagtgat
     6901 cgaactggcg aaaacgaact ggctaaggta aaaagggtgg catttcccgt cataataagg
     6961 acatgccatg attgatttac gcagtgatac cgttacccga ccgagccgcg ccatgctcga
     7021 agcgatgatg gccgccccgg ttggggacga cgtttacgga gacgacccta ccgttaatgc
     7081 tctgcaggac tatgcagcag agctttccgg taaagaagcc gccatttttc tgcctaccgg
     7141 cactcaggcc aacctggtcg ctctgctcag tcactgcgaa cgtggcgaag agtatattgt
     7201 cggtcaggcc gcgcataact atctgtttga agccggtggc gcagcggtgc tgggcagtat
     7261 tcagccgcaa cccatcgacg cggctgccga cggcacgcta ccgctggata aagtggcgat
     7321 gaaaatcaaa cccgacgata tccatttcgc ccgcaccaaa ttactcagtc tggaaaacac
     7381 ccacaacggc aaagtgctgc cgcgtgaata cctgaaagaa gcatgggaat ttacccgcga
     7441 gcgcaatctg gcgctgcatg ttgacggtgc gcgcatcttt aatgccgtgg tggcttacgg
     7501 ctgcgaactg aaagagatca cgcaatattg tgattcgttc accatttgcc tgtcgaaagg
     7561 tcttgggacg ccagtcggtt cattactcgt cggtaatcgt gattacatta aacgtgccat
     7621 ccgctggcgg aaaatggccg gtggcgggat gcgtcagtcc gggattctgg ctgccgccgg
     7681 gatgtatgcc ctgaaaaata acgtcgcacg gttgcaggaa gatcacgaca acgccgcctg
     7741 gatggcggag caactgcgtg aagcaggcgc ggatgtgatg cgtcaggaca ctaatatgct
     7801 gtttgttcgc gtcggggaag aaaatgctgc cgcgttaggc gaatacatga aagcgagaaa
     7861 cgtgctgatt aacgcctcgc cgattgtccg cctggtgacg catcttgacg tctcgcgcga
     7921 acaactggcg gaagtcgccg cccactggcg cgcattcctg gcgcgttaag gagagagacg
     7981 tgccgcaacg cattttagtt ctcggtgcca gtggctacat tggtcagcat ctggtgcgca
     8041 cactcagcca gcaagggcat cagatcctgg cggcggcacg tcatgtcgac aggcttgcaa
     8101 agctgcaact ggcaaacgtc agttgccata aagtcgatct cagctggccg gataaccttc
     8161 cggccctgtt gcaggatatc gataccgtct attttctggt gcacagcatg ggcgaaggcg
     8221 gcgattttat cgctcaggag cgccaggtgg ctctcaacgt ccgcgatgcg ctacgtgaag
     8281 taccagttaa gcaattaatc tttctcagtt cgttgcaggc cccgccacat gagcagtcgg
     8341 atcatctgcg tgctcgtcag gctacggcgg acattcttcg tgaagcgaat gtacctgtga
     8401 ccgaacttcg ggccggaatt atcgttggcg caggttcagc ggcgttcgaa gtcatgcgcg
     8461 atatggtcta caacctgcca gtgttaacgc cgccacgctg ggtacgttca cgcaccacgc
     8521 ccatcgcgct ggaaaacttg ctgcactatc tggtggcgtt gttagatcat ccggccagcg
     8581 aacaccgcat cttcgaagcc gccggaccag aggtgctcag ttatcagcaa cagtttgaac
     8641 attttatggc ggtgagcggt aagcgccgct ggttgatccc catccccctc cccacccgct
     8701 ggatttcggt gtggtttctc aatgtgatta cttccgtacc gcccaccacc gccagggcgt
     8761 tgattcaggg gctgaaacac gatctgctgg cggatgatac cgcgctacgt gcactcatcc
     8821 cacaacggct gatcgctttc gatgacgcgg tacgtagcac gttgaaagag gaggaaaaac
     8881 tggtcaactc cagcgactgg ggctacgacg ctcaggcctt tgcccgctgg cgaccggagt
     8941 acggttattt tgccaaacag gcggggttta ccgttaaaac gtccgccagc cttgctgctt
     9001 tatggcaggt agtgaaccaa atcggcggta aagagcgtta tttctttggc aatattttgt
     9061 ggcagacacg ggcgttgatg gaccgcgcga tcggtcataa gctggcgaaa ggccgcccgg
     9121 agcgcgaata tttacagact ggcgatgcgg tagatagctg gaaagtgatt gtcgttgaac
     9181 cggaaaaaca acttacgttg ttatttggca tgaaagcgcc ggggctggga cgactgtgtt
     9241 ttagcctgga agataaaggc gactatcgta ctatcgatgt ccgcgctttc tggcatccgc
     9301 acggtatgcc ggggctgttt tactggttat tgatgatccc cgcgcatctg tttatttttc
     9361 gcggaatggc aaaacaaatc gccagactgg cagaacaaag cacagattaa tcgacaaaac
     9421 ctgcgttatt ctttcatgtt tcccattgca tcatggggga aatcacggaa gaatgcgcac
     9481 gttattttct caacaacagt ggattgatat gaaggtactg gttaccggcg ccaccagcgg
     9541 cttaggtcga aacgcggtag agtttttatg ccagaaaggc atcagcgtgc gagcgaccgg
     9601 tcgcaacgag gcaatgggca aattgctgga aaaaatgggc gcagagtttg ttccggcgga
     9661 tctgaccgag ctggtttcat cacaagctaa agtgatgctc gcgggcattg atacgctgtg
     9721 gcactgctcc agctttacct caccctgggg gacacaacag gctttcgatc tggctaacgt
     9781 tcgcgccact cgccgcctgg gtgaatgggc tgtcgcctgg ggtgtgcgta actttattca
     9841 tatctcttcc ccctccctgt acttcgatta tcaccaccat cgcgatatta aagaagattt
     9901 tcgccctcac cgcttcgcca acgagtttgc ccgcagcaaa gcagccagcg aagaagtgat
     9961 caatatgctt tcgcaggcga atccacaaac gcgctttact attctgcgcc cacaaagtct
    10021 gttcggaccg cacgataaag tctttattcc ccgtctggcg catatgatgc accactacgg
    10081 cagtattctg ttaccgcatg gcggcagtgc gctggtcgat atgacctact atgaaaatgc
    10141 cgtgcacgca atgtggctgg caagccagga agcctgcgat aagctacctt ccgggcgtgt
    10201 gtacaacatc accaacggcg agcatcgcac actgcgcagc atcgtgcaga agctgatcga
    10261 cgagttgaat attgactgtc gtattcgttc cgtcccctac ccgatgctgg atatgatcgc
    10321 ccgcagcatg gagcgtttag gccgcaagtc agcaaaagag ccgccgctga cccactacgg
    10381 cgtctccaag ttgaattttg actttacgct ggatattacg cgtgcgcagg aagagttagg
    10441 ttatcagccg gtcatcaccc tggatgaagg tatcgagaaa actgccgcct ggctacgcga
    10501 ccacggaaaa ctgccgcgct aatcctgccc gtatttctcc agcaatgctt cggcaatcgc
    10561 ctgagtttct gcatccgctt cgccgttata taacgtcggg cggaaatgca tctggaatgc
    10621 cataatcacg cgccgctgct cgcgcggtgt catatcaggt ttaacgtcat aaccgtagcg
    10681 cgccaaaagc tccagcaatg acgcggtatc taccggtgtg tgcggcgcgc gcccggcaag
    10741 gtaaaagtta acccgctgcg catccggcca ggcaccaatc ccctgctgcg ccagttgctg
    10801 ccagggaaat aatggccccg gatcgtcttt gcgctgcggt gcgatatccg catgtgccac
    10861 tacgttttcc ggcttgatgt gataacgggc aataatatct ttcgccagtg gaataagcgc
    10921 ctgaatctgt gccggttcaa acggggcaaa atatttcact ccggccgatt tttgccagcc
    10981 acggttttcc agctcaatgc caatagaggt gtcgttaagg cgcgttgccc cgcgccaggc
    11041 gctaatcccc gcatgccagg ccagttcttg ttccggcacc agttgccaga tgcgcggttt
    11101 accgttgtat cgcggtggta ccgcagggac cagataatgc gagctgacct gcttatcggt
    11161 cagtgtcgcc agtgagctat caaaatcatc tgcggtgtag tggatcacca gcactttaat
    11221 gcgcggatac gccgcctgcg cctggcgtcg ggtatcaagc tgatatccct ctttctcgac
    11281 aatgcctttt tcgcctgcac accctgccag taacagagcg gcagcgatca gccagaagac
    11341 tcttctcatc gacgcgtttt caccgccgta ccgctgacgc taaccatcag catactgccg
    11401 ttttgcccga ccgtttcgta gtcgatatca ataccgacga cggcatcggc ccccagcgcc
    11461 cgggcctggg agccaagttc ctcaaaggcg atctcccgcg ctttgcgcag ttctttttca
    11521 tacgcaccgg agcgtccgcc aacgatatcg cggataccag caaagaaatc acggaaaata
    11581 ttggcaccta aaatcgcttc gccggtcacc acaccgcaat attcaacgat ggtctggcct
    11641 tccagagttg gggttgtaga aaattgcatg atcgatctcc ttgtttgtta agccttcgat
    11701 ctcaaaagca ttatcggact gatacgctat tattgaaagg atatcattat tatcgtagaa
    11761 cgctttcaga gcgatcgctt ataaggaaat cattatgcgc tacagcaaat tgacaatgct
    11821 tatcccctgc gcactgctcc tcagtgcctg caccacagtc actccagctt ataaagataa
    11881 cggcacacgc agtggtcctt gcgttgaagg aggcccagat aacgtggccc agcaatttta
    11941 tgactaccgc attttgcacc gcagcaacga catcaccgcc ttgcgccctt atctgagcga
    12001 caaactggcg acactgctta gcgatgcctc ccgggataat aaccatcggg aactactgac
    12061 caacgatcca ttctccagtc gaaccacctt gccagacagc gcccacgttg ccagtgcatc
    12121 gactatccct aatcgtgatg cccgtaacat tccattgcgc gtggatctaa aacagggtga
    12181 tcagagctgg caggatgaag tgttaatgat tcaggaaggc cagtgttggg tgatcgacga
    12241 tgtacgctat ttaggcggca gcgttcatgc cacggcgggt acgctccgtc agtccatcga
    12301 gaatcgctaa cgccttgatc aaccaataaa ccccgtaaat tgtccggcat ttcgtgagga
    12361 atgccgacat ttatgctcac cgaccaccgc ccccgttatt ttgtgctatg tttattgaat
    12421 aatgcgcttt gcttttaact tttaaagcag aaatattgca taattattct gtcaaaggta
    12481 ctatcttcga cctcaatagc tatcagactg ccagtatacg agtgtcaatg agtattcaat
    12541 taaacggcat taattgcttc tacggcgcgc atcaggcgct gttcgatatc acgctggatt
    12601 gcccacaggg cgaaacgctg gtgttacttg gccccagcgg cgcgggtaaa agctcgctgc
    12661 tgcgtgtact caatctgctt gagatgccgc gctccggtac gctcaacatt gcaggcaacc
    12721 atttcgattt taccaaaaca ccctctgaca aagcgattcg cgatttgcgt cgtaacgttg
    12781 gcatggtgtt tcagcaatac aacctgtggc cgcatctgac cgtgcagcaa aacctgattg
    12841 aagcgccctg ccgtgtactg gggttgagta aagatcaggc gctggcccgt gcagaaaaac
    12901 tgctggaacg tctgcgtctc aaaccttata gcgatcgtta cccgctgcat ctttctggtg
    12961 gtcagcagca gcgtgttgct attgcccgtg cgttgatgat ggaaccgcag gtactgctgt
    13021 tcgatgaacc gaccgccgca ctggacccgg aaattacggc acaaatcgtc agcatcattc
    13081 gtgagctggc agaaacgaat attacccagg tgatcgtcac ccacgaagtt gaagtggcgc
    13141 gtaaaaccgc cagccgagtg gtgtatatgg aaaatggtca tatcgtagaa caaggcgacg
    13201 cgagctgctt taccgagccg caaaccgaag catttaaaaa ctatctctct cactaagatt
    13261 cgggataaca acaatgaaaa aagttctgat tgccgcgtta attgcaggtt ttagtctttc
    13321 cgccacagct gccgaaacca ttcgttttgc taccgaagcc tcctatcctc cgtttgaatc
    13381 gattgatgca aacaaccaga tcgttggttt tgacgtcgac ctggcacagg cactgtgtaa
    13441 agagattgat tcaacctgta ctttctctaa ccaggcgttt gacagcctga tcccaagcct
    13501 gaaattccgt cgcgtagaag cagtgatggc gggcatggat atcactccgg agcgtgaaaa
    13561 gcaggtactg tttaccaccc cgtactatga caactctgcc ctgtttgtgg gtcagcaagg
    13621 caaatacacc agtgttgatc agctgaaagg caaaaaagtc ggcgttcaga acgggacgac
    13681 acaccagaaa ttcattatgg ataagcaccc ggaaatcact accgttccgt atgacagcta
    13741 ccagaacgca aaactggatc tgcaaaacgg gcgtatcgac ggcgtcttcg gtgacaccgc
    13801 agtggtcact gagtggctga aagataaccc gaaactggcg gcggtgggcg acaaagtgac
    13861 cgataaagat tacttcggca ctggcctcgg catcgcggta cgtcagggca acactgagct
    13921 gcagcagaaa ctcaacactg cgctggaaaa agtgaagaaa gatggcactt acgaaaccat
    13981 ctacaacaaa tggttccaga agtaattcct gatgaatgaa tttttttctt tagcaagcgc
    14041 cgccgggatg accgtcggcc ttgccgtttg tgcattgatt gtcgggctgg cgctggcgat
    14101 gttctttgcg gtatgggagt cggcaaaatg gcgtcctgtc gcgtgggcag gttcagcgct
    14161 ggtaaccatt ctgcgtggcc tgccagaaat tctggtggtg ctgtttatct attttggctc
    14221 ctcgcagctg ctgctgacgc tttcggatgg cttcactatc aatcttgggt tcgtgcaaat
    14281 cccagtgcag atggacattg agaacttcga cgttagcccg ttcctttgtg gtgtcatcgc
    14341 tctgtcactg ctgtatgccg cctatgcctc gcaaacgctt cggggcgcgt tgaaagcggt
    14401 gccggtgggt cagtgggagt ctggtcaggc gctggggctg tcgaaatcgg ctatcttttt
    14461 ccgtctggtg atgccgcaga tgtggcgtca tgcgctgcct ggcctcggta accagtggct
    14521 ggtgctgctg aaagataccg cgctggtcag tttgattagt gtgaatgatt taatgctgca
    14581 aacaaaaagc atcgctactc gtacccagga accatttacc tggtacattg tggcggcggc
    14641 gatttacctg gtgatcaccc tgctcagtca gtacattctc aaacgcattg acctgcgcgc
    14701 gacacgtttt gagcggaggc ccagctaatg tttgagtatt tacccgaact gatgaaaggg
    14761 ctacacacca gcctgacgct aaccgttgcc tcgctgattg tggcactgat tctggcattg
    14821 atttttacca tcatcctgac gctgaaaacg ccggtgctgg tgtggctggt gcggggttat
    14881 atcacgctgt ttaccggtac gccgctgctg gtgcagatct tcctgattta ttacgggccg
    14941 ggccagtttc cgactttgca ggagtatccg gcactgtggc atttgttgtc agaaccgtgg
    15001 ttatgtgcgc tgattgcgtt atcgctgaac agtgcggcgt ataccacgca gctgttttac
    15061 ggtgcaattc gtgcgatccc agaaggtcag tggcagtcct gtagcgccct gggaatgagc
    15121 aaaaaagata cgctggcgat cctgctgccg tatgccttta aacgctcgct ctcttcttat
    15181 tccaacgaag tggtgctggt gttcaaaagt acctctctgg cttacaccat tacgctgatg
    15241 gaagtgatgg gatacagcca gttgttgtac ggacgcacct acgatgtaat ggtgttcggt
    15301 gcggcaggga ttatttacct ggtcgtcaac ggcctgctga cgctgatgat gcgtctgatc
    15361 gagcgcaaag cactggcatt cgaacggcga aattaatgta gctgcgtaca ttcgccgggg
    15421 gggtttgtag gtctgataag acgcgccagc gtcgcatcag acatcagcac ggtgcctgtg
    15481 ccggatgcgg cgtggacgcc ttatccggcc tacatagtgc ataaattctt ataatgaaga
    15541 cggacaactc actaaggtat ccgtcttttt ttatttcatt taaattattt aatcatattt
    15601 attgcatata aattcacttg atggcattgt tatcccatgc cgcagacacg gccaaaaatc
    15661 ataagattga cagacgggag ttccatcatg aaaaagttag ttcttgccgc tttacttgct
    15721 tcctttactt tcggtgcttc tgccgcagag aaaatcaatt ttggcgtttc agccacctat
    15781 ccaccctttg aatctatagg tgctaataat gagattgtcg gctttgatat cgatctggca
    15841 aaagccttgt gcaaacaaat gcaggcagaa tgtactttta ctaatcacgc gttcgacagc
    15901 ctgatcccgt cactgaaatt cagaaaatat gacgccgtaa tctccggtat ggatatcacg
    15961 cccgagcgta gcaagcaggt atcgtttacc acgccctact atgaaaactc agccgtcgtg
    16021 attgccaaaa aagataccta caaaacgttt gccgatctga aaggcaaacg tattgggatg
    16081 gaaaacggca ccacgcacca gaaatatatt caggatcagc accccgaagt gaaaactgtc
    16141 tcctatgaca gttatcagaa tgcctttatc gatctgaaaa atggtcgtat tgatggggta
    16201 tttggtgaca cagcggtggt aaacgaatgg ctgaaaacca atccgcaact gggcgttgct
    16261 actgaaaaag tgaccgatcc gcaatacttc ggcaccggtc tgggcatcgc tgtacgtccg
    16321 gataacaaag ctctgctgga aaaactgaat aacgcgctgg cagcaattaa agctgacggt
    16381 acatatcaga aaatcaatga ccagtggttt ccgcagtaat tgagactgat gacaaaagta
    16441 agattgcctg atgcgctgcg cttatccggc ccacatcaac tctgcaatgt attgatttag
    16501 catgattttg taggccggat aaggcgtaca cgccgcatcc ggcatgaaca aagcgcacct
    16561 gatcagcaat ctgaagccgc acctgcggct tttttattgc ttcaccagca gcgtcagcac
    16621 ttcatagtgc gcggtgtgcg ggaacatatc aaaaagctgt acccgttcga tgcggtaacc
    16681 tggcagttcg cggatatctt tcgccatggt ttgggcgtta cagctggagt agataataaa
    16741 acgcggtgcc attgttgaga gataatcaca tagcggttta ccaatgccgc ggcgcggcgg
    16801 attaaccagc accagctccg gcacctcccc ctgagcggtg gcaaactgag tggagtccag
    16861 cgcctgaaat tgcaaacgcg ttaagcccag ttcagcggct gactgctttg cacaggcaat
    16921 ggcctctgat gcaatttcga tcccggttaa ctgcatgtca ggcgtcgcgc agtgtaaacc
    16981 aaagccaccc acaccgcaga acagatccca catatgttta actggcagct gccgtaccca
    17041 gtcgcgcgcg gtagcgtaca gctggctggc gaccgccgga ttagtctgga agaaactttg
    17101 cggacggatc cacagcggta cgtcattaaa acgctctgcc agcgcctgtt gttcagtaag
    17161 gtagatctcc gtctcccctt ccataatcgc catatgtacc ggctgaatat tgacggtaat
    17221 aactttcagt tgcggtagtt gttcctgtaa ccacggcagc gccttacgca gttgtgccag
    17281 tttggtttca gaacgcagta caaagcgcag catcatgccg ccatcgctct ggctttcagt
    17341 cagcagaatg tatttcagtt cgccacgctt acgcgccacg ttgtagggtg tcagccccgc
    17401 acgggcgata aacggtttta gcgccgcaaa aacgggcgca aatgaggcag gataaagcgg
    17461 gcagtcacaa aggtcttctg gtgtgccgtc gcgatgcagc ataccgagca gtggtttttc
    17521 aacgctacca ctcaccacca ttttggcttt attacgaaac ccttgttccg ggcctgacac
    17581 cggcgcgcac cattcctcaa ccggaaaatc ggcgagcaga tttttaagat cggcggtttt
    17641 agcggagagt tgctctggaa tcggctgcgt tatccactga caggaacgac agcgacccgc
    17701 gtcgtaaagt gcgcactgca tacattgacc ttcacatcat caggggcgac gattatacac
    17761 attattgcaa ctggaagaac cgccgactgg tcgagggaac gaacagcagc atcaggatga
    17821 gcatatccgg cagcttctgc agcatcaggc tatggaagat ttcacgtttt gattcaccgg
    17881 gaatgctgaa cagctccgga taaccgtacc ccagcgaagc cgcccacaag taacttgcgg
    17941 cggtgatttg tgtcagcaga tatagccagc gcgcccagcg acgccctttc accagtgaaa
    18001 acgcacacca gatctcaatg aaaaccagca ccagactgct taaaaagacc agcgttaaac
    18061 tctaggtttg tacgctgcga tgaatgaact cgccaatacc gcgcacgccc agcgtattga
    18121 aaatcatcag cacgtcgagg ccacggatca taataatggc gagcgccgcc acctgcacca
    18181 gcgcaggcac attcaggcga gcatgagatg aagatgtttt cttaaaaaat cccaacgttt
    18241 cgtcttccat gaaaacaatg ccgcggcatg cgcggcatta tgtagccagg ttggcaaatt
    18301 ttagtgtctt cagccatgtc ttgcacgctg gatatcgcgt atccgctgtt tttctgcgcg
    18361 agccatcaga taccaggcga taaatccgac aattccgacc gcaccgagaa ttaacgttgc
    18421 aagggcgttg atttccggat tcacccccat ccgcacgctg gaaaagacca gcatcggtaa
    18481 cgtggtggct cccggcccgg aaacaaagct ggcgatcacc agatcatcaa gcgacaaagt
    18541 aaaagccagt aaccagccag aaatgatcgc gggcatgatc atcggtagcg taatgacgaa
    18601 aaacactttc agcggcgtcg caccgagatc catcgctgct tcttctatcg agcgatccag
    18661 ttcccgcaat cttgaagaaa taacgaccgc cacataagcc gtacaaaaag tgacatgcgc
    18721 cagccagatg gtgagcatac cgcggtccgc aggccagcca atggcatgag caagcgcgac
    18781 gaataataac aacagcgaca agcccgtgat gacatctggc atcaccagcg gcgcggtgat
    18841 cataaaggca aagccatttg atccgcgaaa cctgccaaag cgcaccagca ccaccgccgc
    18901 aatagtcccg aggatcgccg ccgccgttgc cgcacaggca gcaattgtca ggcttaaacc
    18961 aaccgcactc atcatcgcgt catcgcgcaa taactcacca taccagcgcg ttgaccagcc
    19021 ggcccacacc gtcaccagct tcgagctgtt aaacgaatag ataaccagca tcagcattgg
    19081 cgcgtagaga aaggtaaagc ccagcagcaa aatcacaatc cgccagggcg aacgaactac
    19141 cggtaaatta ttcatccgtg ttctcccacg cttttttgct ggtgtttgtg gaaccacatt
    19201 atcggcacaa ttagcagcag caacatgatg atcgctaccg ccgaggccac cggccagtcg
    19261 cggttattga aaaactcttg ccatagcacg cgcccgatca tgatgctgtc cgggccaccg
    19321 agcagttccg ggatcacaaa ctcgcccaca gccgggataa acaccagcat cgatccggca
    19381 ataatcccac ctttagtcag cggcacgatc acagtaaaga acgttttcag aggtcgtgca
    19441 ccgagatcca gcgctgcttc caccagcgaa taatcaatac gaatcaacgc ggtataaatc
    19501 ggcagtacca taaacggcac gtaagcgtaa acaatgccga tataaacggc cagattggta
    19561 tgcagaatgg tcagcggttg atcgataacc cccagccaca gcagaaaatt attcagcaca
    19621 ccgttgtttt ttaatattcc catccaggca taaacgcgga tcagaaacga ggtccacgac
    19681 ggcaggatca ccagtagtaa taagatatta cgggtcgaag gcttactgtg cgccaccgcc
    19741 cacgccagcg gatagccgat cagtaaacag caaaatgtcg aaatcgcagc cacctgtagc
    19801 gactggagat aagtatcgaa atagagcgga tcgtcggtca gttgcagaaa attaccgaga
    19861 ttaagagtga tggacagttg cccgtcagcc cactccatca gttcggtata aggtggaata
    19921 gcgcgcgcca tctccgccag gcttatttta aagacaatca gaaatggcag cagaaacagc
    19981 aagatcaacc agatatatgg caacgcaatg accagtttgc gcccatgctt catttgcagc
    20041 tgcgacaacc acagcttaaa tccgcccggc ggtttcgact gggcagcagg ttcaagtgta
    20101 ttcatctttg ctccttaaac cgtcagcacc acacagctgt ccacttccca gcacaaacgc
    20161 acttcgtcgc cccaggtcgg taacccttta cgatgacgat gggcgttttg tagctgggcg
    20221 ctaatcatct gcccactttt cagacgaacg tgatacaccg aaagatcgcc gagataggca
    20281 atgtgtatca cctcccccac cgcgaagtta caaccattgg cgggcggctc ttcgcaaagc
    20341 atgatttttt ccgggcgcag cgctacgtgt accggcacgt tatcgaccac tgaagcatcc
    20401 gcatcgactt tcagtggatg caccagcccc ggcgaatcaa gcaccaggcc atcttcctga
    20461 cgctctttga gtacaccttc aaagacatta accgaaccga taaattcagc gctgtagcgg
    20521 gtagttggat gctcgtagat ctcttccggc tcgccaatct ggacaaattt cccgcgattc
    20581 ataatggcga tacgccccgc catggtcatc gcctcttcct gatcgtgggt gaccatcaca
    20641 caagtcacac cgacgcgctc cagaatatcc accacttcaa gctgcatccg gtcacgcagc
    20701 tttttatcca gcgcgcccat cggctcatcg agcagtaata gtttcgggcg tttggcaagg
    20761 cttcgggcca gggccacacg ctgtcgctga ccaccggaaa gctgatgcgg tttgcgtttg
    20821 gcgaactcct gcatgtgcac cagcccaagc atctcgttga cccgggtggc aatttccgct
    20881 ttcggtagtt tgtcctgttt caggccaaag gcgatgttct gctccacagt catatgggga
    20941 aacaatgcgt aagactgaaa catcatattg atggggcgta gataaggtgg cacctgtgac
    21001 aaatcgacgc catcaagcat aatctgtccg gcagagggtt gttcaaaacc tgctagcata
    21061 cgcagcagcg tggatttacc acagccggaa gcgcccagca gcgcgaagat ttcacctttg
    21121 tagatagtca ggctgacatc atccaccgca tgttgaccat cgtaggattt ggtgaggttg
    21181 cggatttcta atagcggcgt cagcgcctta cgggttttcg cctgcgggcg agggatagcg
    21241 tcattcacgg ctgctctccg gcattgaatg tgtgcaagcg caccatgatg gtgcgaattg
    21301 ccggcgggtg tggtgcgccc gcccggcatc taaggctgcg gattattttc cgctcttcac
    21361 tttggtccac gcgcgggtgc gcacacggtc gattttcgga tcctgcactt tcagagtgaa
    21421 cagcttcgca cggacatccg caggcggata aatacccggg ttatcacgga cttccgcact
    21481 caccagcggc gtggctgctt tattggcgtt ggcatagaac acatggtcgg aaatatgcgc
    21541 tactacatcc gggcgcagca ggtaattcag gaactgatag gcttcgtctt tgtttttggc
    21601 atccgcaggc atggcgaaga catcaaagaa cgccatcgcc ccttcttttg gaatcgagaa
    21661 cgagacatta acgccattct tcgcttcctt cgcgcggttt gacgcctgcc agacatcgcc
    21721 cgcccagcca atagcgacgc agatatcgcc gtttgccagg tcgttaatgt attgagatga
    21781 atggaaataa cgaatgttcg ggcgcagctt taacagcaga tcggtagccg gtccggtgta
    21841 atcatccgct ttagtgctgt tgggatcttt gccgagataa ttcagcacgg tagcaaaaac
    21901 ttcttctggc gcatccagga aagagacacc gcagcttttc agtttttcca gattttcagg
    21961 cttgaggatc aagttccagc tatcgacggg cgcgttttcg cccagcaccg ctttaacttt
    22021 atcaacgtta tagccaatcc cggtcgtcgc ccacatatag ggcatagcaa atttattgtc
    22081 gggatcgtgt ttggcgacca acttcagcag ttccgggtcg agattcttcc actccggcaa
    22141 tttgctttta tccagcggct ggaaaactcc cgctgtcagc tggcgttcaa gaaagcttgc
    22201 tgacggcacc accaggtcaa agccggtgct cccggccatt aatttgcctt ccagtacttc
    22261 gttagagtcg aaaacatcgt agacgacttt aataccggtt tctttttcaa aattggccac
    22321 cgtgtccggg gcgatataat cagaccagtt ataaatgtgg agtgtttttt gttcagccgc
    22381 gagcgtgccg acagagacgg ccatcagagc acccgcaacc agacccgata gccatttttt
    22441 atttaaggcg gtcatatctc tttccttctg aaagttcgtt aacaaacgaa caaaagttgt
    22501 cacactgaaa gatatgcaat aaacgtgcat attttttatc ggcgcggtgt aagaaaagag
    22561 aactctctcc cggccagcac tgctcgctat ataaaagcat cgcaagaata actttagcca
    22621 gggtttgaga ctatagctcg gattaaaaaa cggtatttaa caaattttta tgcgtatttg
    22681 ctctatgtga taaagccaaa tcgacacaag agaagtgaaa taatctttaa gaaaaggtta
    22741 aaaacagcag ataattttgc agcgcgcagc cactgcacca gcgactgcgt gctcgaaagg
    22801 agttatcagt ggaggaaata gttttcggta acgttgtttt gcccttcatc atcagtcggt
    22861 aacagcagtt gatgggcatt agcttcaaga atgaccatcg aaatctgctc ttcgctctga
    22921 cgcacgaacc atgcaaactg ctcataggtt acgccctgca taacggataa agactggcaa
    22981 accaccagct ttggcagatt atcatcctgc atatcaagaa atgctttcac ggtcagcgaa
    23041 ctggcattga tggcagataa atccgccgcc agcggcaata cggctgaagg tcggacttcc
    23101 gccatggcag aaaacaggat cgtgttatcg atcagatcga ttttggcatc aaagacaccg
    23161 tcgaaattct gcatatgggg cagatgcaga gcctgacagt tatcacattc aaaaaaactc
    23221 atccccaggt catcgagcca ttgacgcagc gtatccagac caggaacgac cagcgatgtc
    23281 ataattttgt acaacctctt tcgataaaaa gaccggcaca gcttacgcaa aaagcgcagg
    23341 caaaaccatg atcagtaatg tgattgcgac taaccacccg ttttcaggca atattctgtc
    23401 gtagcgtggc gttcgatcca gcggatcatt ttacccgcga tgtcgatacc ggtggttttt
    23461 tctattcctt ccagccccgg cgacgcattc acctccatca ccaacggccc gcgattagca
    23521 cgcagaatat ccacaccagc aacgtccagc gccatcgttc gcgcggcttt tatcgcgatt
    23581 tcacgctcct gtggtgtgat actggcgacg cttgccgcgc cgccacgatg caaattggaa
    23641 cgaaaatcgc cctctttcgc ccgccgttca atcgcagcga ccacttcatc gccaacaacc
    23701 agacagcgga tatcgcaccc ttgcgcctct ttgatatatt cctgcaccag aatatgcgcg
    23761 ttcagaccgc ggaaagcgtc aatcacgctt tccgccgcct gacgcgtctc cgccagcacg
    23821 acgccaattc cctgcgtgcc ttcaaccagc ttgaccacca gcggcgcacc accgaccatg
    23881 tcgattaaat cgctggtatc atccggcgaa tgcgcaatgc ccgtgacagg caggtcgatg
    23941 ccctgacgcg ccagcagttg catggaacgc aatttgtcac gcgcccgggc aatggcgacc
    24001 gactcattga gcggatagct ccccagcatc tcgaactggc gcagtgccgc cgtcccataa
    24061 aaggtaatgg cggtgccaat acgcgggatc actgcgtcaa aatggggtaa cttgcggcct
    24121 ttgtagtgaa tagaagacgc cgcaggattt atgttcatgt agcaagaaag cggatcaaga
    24181 atttcaacca ggtgaccgcg ctgtatagcg gcttcacgca gccgcttaca cgaatagagc
    24241 gttccatccc gggacaatat ggcaattttc accctgcacc tctctgtcag acctggtcaa
    24301 aagcccgcgt atcatacatc gacgtggcgg ttttagcgcg tcgcccaacc ctgtttgtgc
    24361 aaataatcca gaataaatgg gcggctttct ttaatgattg ttcggcggat atgatcgctc
    24421 caggtatccc ggcgattatt gctgccacgg gtgaggtaat attccgccag ttgctcgtca
    24481 tactgcgcca gtgcgccttt atccagcggt tgatagctgt tttcatgcac caaaatggag
    24541 gccggtaaac gcggcttaag atccggattt tccgcaggcc agccaaggca cagcccaaac
    24601 agcggcagaa catgctgcgg taatttaagc agtttcgtca ccgcttcaat attattgcgc
    24661 aggccgccga tatatacccc gcccaatccc agcgattccg ctgcgattaa tgcattctgc
    24721 gccatcattg ccgtatcaac gacaccgagc aacagttgtt ccgccaggcc gagctgagca
    24781 tccggacaga tctgtaaatg gcggttaaag tcggcacaga acacccagaa ctccgccgct
    24841 tgcgctacgt gtttttgccc gccggtcagc gtcaccagtt cttcacgtaa cgctttgtcg
    24901 gtaatgcgaa taatgctact gcactgcaaa aaactggaac tggacgtcgc acgggcgctg
    24961 ttaataatcg cctcacactg cgcgtcggaa atgggttcat cagtgaaatg gcgaatggag
    25021 cgatggccac aaataagttc aatggttggc gtcattatct ttttctcttt ctgaacgtga
    25081 atattgcggt ggacggttca tcagctgtgg ggcaagacgt tttgccacct gaagaataac
    25141 caccaccgca gcgggaagca tgagcaaaac accgagaaaa atcatcagaa tctgcacttc
    25201 tggccgagaa aatggctcag gcagcgacag ggagtcgctt accgacagca gcgccaccgc
    25261 cagtagcatc attccgataa attccagtat caacacgcct ttaggcaatt taccgatcgc
    25321 gcgcatacgc ttccctctgc aaagtgagcc ttcagtctaa aacttttcac tgtattgtgt
    25381 ttaacagtta tagcttttag caattaatgc aacaggttaa acctactttc agcgaataca
    25441 ttttagcgtg atcattacag gcataaatct atgaggagag aaataatgca aaccgttatt
    25501 tttggtcgtt cgggttgccc ttactgtgtg cgtgcaaaag atctggctga gaaattgagc
    25561 aatgaacgcg atgattttca gtatcaatat gtagatattc gtgcggaagg gatcactaaa
    25621 gaagatctac aacaaaaggc aggtaaaccc gtagaaaccg tgccgcagat ttttgtcgat
    25681 cagcaacata tcggcggcta taccgatttt gctgcatggg tgaaagaaaa tctggacgcc
    25741 tgatcgtctg acaagccctc gcgttgaggg ctttactgtt tttttctgtg ctgtggttta
    25801 aacaaactac tgataaataa gaaacacagt gcccccagcg cacaccagaa caccgcgctt
    25861 agtaaccatg ccagctcttg ccagaatgag cgcgtcggtg aaaaaaacag ccgcataatg
    25921 agcatcgaac agggtgccgc cagcattgcg ccaaacagag gtttcaggac ttctctacgc
    25981 tgtgaaaaga agctggccac tgctccagga agaatgaaaa atagcaagcc gatttcagga
    26041 tgcccggcag cccgaaaagc gcctttcatg tgcgtcgcca gaaaaaggca caccacaatg
    26101 aagaggacaa aacagcagat tgcccccgcc caacgttgtt tatgtttcac tcgttcctcc
    26161 tgacactgcg tctatcgaac acatttttcg ccagtgtggc gttcagtaag ataaagccgc
    26221 ttcgcattcc atgctaatat aggccaacgc aattcatata gccgttgata cctaatgtga
    26281 ttacactagt aaaatatatt gttactttac tatcgtttag gtgcgctgaa tgaatctgcg
    26341 ccctgaattc tggtaaaaaa cattatcgta aattaccatt tctttcaaca gcttactagt
    26401 aaacaagaag ttagcctccg tgaatataaa cgtcgccgaa ttgttaaatg ggaattacat
    26461 tctgttatta tttgtggtcc tcgcgcttgg gctatgtctc ggaaagttac gacttggttc
    26521 gatccaactg ggtaattcca ttggcgtttt agtcgtatcg ctgttattag gccaacaaca
    26581 tttcagcatt aacaccgatg cgcttaatct tggctttatg ctgtttattt tctgcgtcgg
    26641 ggtcgaagcc ggaccgaact ttttttccat tttttttcgc gatgggaaaa attacctaat
    26701 gttagcactg gtgatggttg gcagtgcgct ggtgatcgcc ttagggttag gtaagctgtt
    26761 tggctgggat attggcctga cggccggtat gttagcaggc tctatgacgt cgacaccggt
    26821 tctggtcggt gctggcgata cactgcgtca ttccggcatg gaaagcaggc agctctcact
    26881 ggcactggat aatctgagcc tcgggtatgc cttaacctat ttaatcggtc tggtgagttt
    26941 gattgttggt gcgcgttact tgccgaaatt gcagcatcag gacttacaga ccagcgccca
    27001 gcaaatcgcc cgcgaacgtg gcctggacac tgatgccaac cgtaaggttt atttaccggt
    27061 gatccgcgcc tatcgcgtcg gcccggagct ggtggcctgg accgacggca aaaatctgcg
    27121 tgaactgggt atttatcgac aaaccggctg ctacattgaa cgtattcgac gtaacgggat
    27181 tctggcaaat ccagacggtg atgccgtgct acaaatgggc gatgaaatag cgttggtagg
    27241 ctatcccgac gcccatgccc gactcgatcc cagcttccgt aacggtaaag aagttttcga
    27301 tcgtgacctt ctcgacatgc gtatcgtcac tgaagaagtg gtcgttaaaa accataacgc
    27361 tgtaggtaaa cgtctcgcac aactgaagtt gaccgatcac ggttgcttcc ttaaccgcgt
    27421 cattcgtagc cagattgaga tgccgataga tgacaacgtc gtgcttaaca aaggtgacgt
    27481 tttacaagtc agcggtgatg cccgtcgcgt aaaaaccatc gccgatcgca tcggctttat
    27541 ctcgattcac agccaggtca ctgacctgct ggcattttgc gccttctttg ttattgggct
    27601 gatgatcggg atgatcacct tccagttcag cacattcagt ttcggcatgg ggaacgctgc
    27661 cgggttgtta ttcgccggaa ttatgctggg ctttatgcgt gctaaccacc cgaccttcgg
    27721 ttacattccg caaggtgcat taagcatggt gaaagagttc ggcttgatgg tgtttatggc
    27781 aggcgttggt ctgagcgccg gtagcggtat taataacggc ctgggcgcga ttggcggtca
    27841 gatgttgatt gccggattaa ttgtcagtct tgtgcccgtg gttatctgtt tcttgttcgg
    27901 tgcttatgta ttgcgaatga accgcgcact gttgttcggc gcaatgatgg gcgcacgcac
    27961 ctgcgcgccg gcaatggaga tcatcagtga tacagctcgc agtaacatcc ctgcgctggg
    28021 ctatgcgggc acctacgcaa tcgccaacgt cctgctgacg ctggcaggga caatcatcgt
    28081 catggtatgg ccaggattag gataaaactg aagttgccct gaaaatgaaa tttttttgca
    28141 caaccgcaga acttttccgc agggcatcag tcttaattag tgccactgct tttctttgat
    28201 gtccccattt tgtggagccc atcaaccccg ccatttcggt tcaaggttga tgggtttttt
    28261 gttatctaaa acttatctac taccctgcaa ccctctcaac catcctcaaa atctcctcgc
    28321 gcgatagcgg cttacggtcg gtgacaaaat gcagcgtcat cccctcaata aacgcatcaa
    28381 gcgcgcgggc ggttccgggt tcaaaccatt gttcgagcgt ttgctgactg cgctgcatcc
    28441 agttttgcat taccgttttt aatagcggtt ttcggctagc cagcgcgtag agctggtaca
    28501 tcagctccat attatccggc gttgcaacct gtgagctgta gatcatatcg gtgatagcct
    28561 ggcatgcgcc ttgagcatca ctaacatcgc taaaaaatgc ctgatattgc cgggacataa
    28621 tctcagtaaa actgctgaac gcctccagca acaactcatc aattcctgaa aaatagtagg
    28681 tcatcgatcc caacggtacc ccggcaaggg tagcaatttt gcggtgcgta acagcatgta
    28741 ttccgtaaag tttcaccgcc tccagcgtgg cctggataat tttttctcgc cgttgcggat
    28801 cgttagcgcg acgcatgtca tttcctctct ctgcaattgt gtacaaatgt acacaacctt
    28861 gctaatgttg tcttccctct tcttattttg acgtgatcta tgaccgtaaa ttcttcacgt
    28921 aatgcattga aacgccgaac ctgggcgctg tttatgttct tctttttgcc aggcctgtta
    28981 atggcgtcct gggcaacccg tacgcctgct atccgcgata ttctctctgt ctcgatcgct
    29041 gaaatgggcg gtgttctctt tggtctgtcg atcggttcga tgagcggtat tctctgctcg
    29101 gcgtggttag tgaaacgctt tggcacgcgt aatgtcatcc tggtcacgat gtcctgcgca
    29161 ttgatcggga tgatgatatt aagtctggca ctctggctga catcgcccct gctctttgcc
    29221 gttggtctcg gcgtctttgg ggcaagtttt ggttctgcgg aagtggcgat aaacgttgaa
    29281 ggtgctgccg ttgagcgaga aatgaataag acggttttgc cgatgatgca cggtttttat
    29341 agcctgggca cgctggcagg cgcaggtgtc gggatggcac tgacggcctt tggcgttccg
    29401 gcaacggtgc acattttatt ggcggcgctg gtaggtatcg cacctattta tatcgccatt
    29461 caggcaatcc ctgacggtac gggcaaaaat gctgccgatg gcacccagca tggcgaaaaa
    29521 ggcgtacctt tttatcgcga tatccagttg ctgctgatag gtgttgtggt gctggcgatg
    29581 gcctttgccg aaggttctgc caacgactgg ttacccttat taatggttga cggtcacggt
    29641 tttagcccta cttccggctc gctgatttat gccggtttta ccctggggat gaccgttgga
    29701 cgcttcaccg gcggttggtt catcgaccgt tacagtcgcg ttgccgtggt tcgggccagt
    29761 gcactaatgg gggcgttggg tattgggctg attattttta tcgatagcgc ctgggtcgct
    29821 ggggtgtctg ttgtactctg gggactgggt gcctcgctgg gcttcccgct gaccatttct
    29881 gccaccagcg ataccggccc cgatgcacca acccgcgtca gtgtggtagc aacgaccggt
    29941 tatctggctt tcctcgtcgg gccgccgctg ctgggctatc tcggcgaaca ttatggatta
    30001 cgtagtgcaa tgctggttgt actggcgctg gttattctcg cggctattgt cgcgaaagcc
    30061 gtcgccaaac ccgataccaa aacgcagacg gcgatggaga atagttgatg agcattaaat
    30121 taattgcggt agacatggat ggtactttct taagcgatca aaaaacctat aaccgtgagc
    30181 ggtttatggc tcagtatcag caaatgaaag cacaaggcat tcgctttgtg gtcgccagcg
    30241 ggaatcaata ttatcagttg atctctttct tccctgaaat tgcgaatgaa attgcctttg
    30301 tggctgaaaa cggcggctgg gtagtgagtg aaggcaaaga tgtttttaat ggcgagctgt
    30361 cgaaggatgc ctttactact gtcgtggaac atttgctgac gcgcccggaa gtggaaatta
    30421 ttgcctgcgg aaaaaatagc gcctatacgc tcaaaaaata tgacgatgcc atgaaaacgg
    30481 tggcggaaat gtattatcac cgtctggaat acgtcgataa ctttgacaac ttagaagata
    30541 ttttctttaa gtttggcctg aatctttccg atgaactgat tccacaagta caaaaagcat
    30601 tacatgaggc catcggcgat attatggtgc cagtccacac cggcaacggc agcatcgatc
    30661 tgattatccc cggcgtacat aaagccaatg gccttcgcca actgcagaaa ttatggggaa
    30721 tagacgacag cgaagtggtg gtctttggcg atggcggtaa cgatattgag atgctgcgtc
    30781 aggcaggctt tagttttgca atggaaaatg ccggcagcgc ggtcgtcgca gcggcaaaat
    30841 accgggcagg ctccaataac cgtgaaggcg tactggatgt gatcgataaa gtgcttaaac
    30901 acgaagcgcc atttgaccaa taaaaacggc agcacgcccg gcaacacaat acctgaatga
    30961 aggctgcata attcgcggaa attaacccgc tgcattattt caggcgaaat gattatgaaa
    31021 aattgtctac tgttgggcgc gcttttaatg ggctttactg gtgtggcgat ggcgcaaagt
    31081 gtcaccgtcg atgtgccgag tggatacaaa gtggttgtgg ttcctgattc agtcagcgtt
    31141 ccacaagcgg tcagcgtcgc gactgtgccg caaaccgtct atgtggctcc tgcgccagct
    31201 ccggcttacc gcccgcatcc ttatgttcgc catcttgcca gcgtaggtga agggatggtt
    31261 atcgaacacc agattgacga ccatcaccat taattcgaga atgcctgatc gcacaaagca
    31321 atcaggcatt tttttaccct tcgtgagaat ttcccatctg tttatctttt aaaaagataa
    31381 ccatcagcga cagccacaaa attccgttga caaggttgaa gagattaaac tgtccgttgc
    31441 ccccgttcag ccaggcatgt ttgctgattt caataccaac ggtaaagatc agcatttgca
    31501 gcattcccat cgccgcagaa accgtacctt tactcatatc gctggcaaac agggttaatc
    31561 gcaccagtcc cgcattcgcc agaccaatac cgaaagcata aatacttaac ccggcagtca
    31621 tccataaata cgcgtgcgat gagataaccg ttgccgcagc agcgaccaac agaccaatca
    31681 taatcggcca gccgcccata attatcagcg aacgtacggt gcggcgcgag gtcaaacgcg
    31741 ctaacagcaa gttacccgca attaacgccc cgaaaatagg cacttgcagc aagccatatt
    31801 catagctgct caactgctcg ccggtaatga tgataatcgg cgactgggcg atccacgcca
    31861 gcaatggcag gctaacgaat cccagcgcca gcgcccccgc cacaaagcgg ccgttcttca
    31921 gcaccagctt atagtcacga ccgagttctt tcagtgacag tttctcgcct atacgcgtgg
    31981 cggtttcagg catggctcgt tgcagaccga aaaaggagat cgctgccaat gcggcaaaca
    32041 aaacaaacat cccctcccag ggcagcacat ggatccacgc cgcgcccacc agcggaccaa
    32101 gtagcggagc aatcagcgcc acgttcgcca tcagcgcggt gatcttgata caaaccgcct
    32161 cttcgaagga ttcctgaatt gcggcgtatc ccacagcgcc aatgaaacag aggcttatgc
    32221 cctgcaagaa gcgcaacagg gtgaattgtt caatgttttg cgccagcaat attgccagac
    32281 aggtgatgat aaaccacacc acccccgcca gcatcaccgg acggcgacca atacgatccg
    32341 acagcggccc caggagccat tgtaaaaaca tcccgcccgc cagatatgcg gtcattgaag
    32401 taggaaccca atcaatgccc gcctgatatt gttccaccac ggccaacata ccgggttgaa
    32461 tcatatcgtt gccgatatag gttgaaaatt cgtaaagcac cagacagaga gggaaaagta
    32521 acgcctgacg tccaagcctg gcaccggaag ctaatttatt ttgcatgcaa tttcttcgcc
    32581 aataataatc gcgcagagtt taataaaagc acagctaacg agaaagcgaa ttttgtagct
    32641 gaaaccacgg ttaagcacat tcttacatta ttgcgagtat agctacgctt tctttaagtt
    32701 ttatttaacc tatgcccgtt acaattaccc accgtaaaca ggccgcttga gggaaataag
    32761 acgatgccgc tttacccagt ttaacctgca ctttattctc aacgacttgc ctgtattggc
    32821 tcccttttaa tcactttgcg tcgggaagtt atgctggaaa atttgaatct ctctctattc
    32881 tctcttatta acgcgacgcc agactcggct ccgtggatga tctcgttggc gatttttatt
    32941 gctaaagatt tgattaccgt ggtaccgctg ctggccgcgg tgctttggtt gtgggggttt
    33001 acagcacaac ggcaactggt gataaaaatc gctatcgcgc tggcggtcag cctgtttgtg
    33061 tcctggacga tgggacatct ttttccgcac gaccgaccct ttgtcgaaaa tatcggctat
    33121 aacttcctgc atcatgcggc ggatgactca ttcccaagcg atcacggtac ggtgattttc
    33181 acctttgcac tggcattttt atgctggcat cgcctgtggt ccggctcact tttaatggtg
    33241 ctggccgtcg tcattgcctg gtcgcgcgtt tatcttggcg tccactggcc gctggatatg
    33301 ctcggtggat tgctggcagg tatgattggc tgccttagtg cccagattat ctggcaagcg
    33361 atggggcata aactctatca acgtctgcaa tcgtggtatc gcgtctgttt tgcattaccg
    33421 atccgcaaag gctgggtgcg tgactgatct ctgctaaaaa gtgtagtatt gagcggctcg
    33481 cttcaataac tattcagagg gattatggaa acacgtcgcg aagagcgtat cgggcagctg
    33541 ctgcaagaat taaaacgcag cgataagtta catcttaaag acgccgccgc cctgcttggg
    33601 gtttcggaga tgacgattcg tcgcgatctg aacaaccaca gtgcgcccgt cgttttgctc
    33661 ggcggctata ttgttctgga accgcgcagt gccagccatt acctgttaag cgatcaaaaa
    33721 tcccgcctgg tggaagaaaa acgccgggcg gcaaaactgg ctgcgacact ggtagaaccc
    33781 gatcagaccc tcttttttga ctgtggcacc accacgccgt ggattattga agcgattgat
    33841 aatgaaatcc cttttaccgc cgtttgttat tcgctaaata cctttctggc gctgaaagag
    33901 aaaccccatt gccgcgcatt tctgtgcggt ggtgaatttc acgccagcaa cgccattttc
    33961 aaacccatcg attttcagca aacgctgaat aatttttgcc cggatatcgc tttttattct
    34021 gcggcgggcg tgcatgtcag taaaggcgct acctgtttta atcttgaaga gttgccggta
    34081 aaacactggg ccatgtcgat ggcgcaaaag catgtgctgg ttgtcgacca cagtaaattt
    34141 ggcaaggtgc gtccggcgcg catgggtgac ctgaaacgct ttgatattgt ggtgagcgat
    34201 tgttgcccgg aagatgagta tgtgaagtac gcgcagacgc agcgcattaa gttgatgtat
    34261 taatgacgta taaccggatg acgtttcgcg ccatccggtt atcagaagat taagagaacc
    34321 agctgccgaa ccactgatgg aatttcatca tcacgaaatc ccacatccga ccaaagaatc
    34381 cgccctcttc cacattttcc atcacgatca gcggacgctg ctcaatggat ttaccgttaa
    34441 gctggaaatc aatggtcccg acaacctgac cttttttcag cggtgcggta agctgcggtt
    34501 ccgttaacgt ataactcgct ttcaggtttt tcagctgccc acgcggtatg gtcactgagc
    34561 ccgcttcacc tgccccgaga ttcacttcgc tcttatcacc aaaccagacg cgctgagtca
    34621 caaaggtggc atcaggttta attggcgtca cggtttcaaa gaagcggaaa ccccaggtca
    34681 ataatttctc agactcatta aaacggatac ggtcagtttt cgcccccagc actacggaga
    34741 ttaaacgcat atcgccctgg gtagccgaag caaccagatt atatcccgcg cctgccgtgg
    34801 ttcctgtctt catgccatca acattcagat tgctgctcca cagcagacgg ttacggttag
    34861 gctgacgaat tttgttgaag gtgaactctt tctctttatg aatggcgtac tcttccggca
    34921 catcgtggat caatgcttta cccagcaatg ccatatcgcg cgcggtactg aactgccccg
    34981 gcgcatccag accgtgcacc gtctggaagg tagtgttggt cagacccagt tttttggcat
    35041 aaccattcat caaaccaatg aatgactcct ggctcccggc aacgtaatcg gccagcgcaa
    35101 tacaggcgtc attaccggac tggataatca cacctttgtt caagtctgcc accgaaacct
    35161 gatcgcccgg tttgaggaac attaccgatg aaccacgcag tgccggattt cccgtcgccc
    35221 aggcatcttt accgaccgtc accatatcgg tgagtttaat cttatcggcc ttaagcgcct
    35281 gcccaaccac atagctggtc atgattttag tcaggctcgc gggatccagt ttctcatccg
    35341 cgttgccttc tgccagcact ttaccgctgg cgtaatccat taaaatccat gcacgcgcat
    35401 ccacgctcgg cgcttcaacg gtttgttccg ccgcgaatgc cgttggggca aaaaggaata
    35461 aaaatgcaga acccgctgca agaccacgaa ggagagagga gtattgcgtc ataaatgcca
    35521 cccgagtatc cattcaaaaa aaatacgtta tatcgccgta tgataagttc ggtgattaat
    35581 aacgcacaca caaacttaaa gaaaccgtaa gttggtaaag tttttaaagt ttatgcaata
    35641 tcctgccgct tcgctgcttc tggtgtcatt tttttgccag ctgttgctta aaagttaact
    35701 ttacttcacc atcggagtcc tgttcagcct gtggctgcct gtcggaaaag gggtgagtat
    35761 gattacgctg tggggtcgga ataattcaac caacgtaaaa aaagtattgc tgacgctcga
    35821 agaactggaa ctaccttatg agcaaattct cgcgggccgt gagtttggga taaatcacga
    35881 tgctgatttt ctggcgatga accctaacgg gctggtgccg ttgttgcgtg acgacgaaag
    35941 tgatctcatt ctttgggaat caaacgccat tgtccgctat ctggcggcac agtacgggca
    36001 aaaacgtctg tggatcgact caccggcacg tcgtgcggaa gcggaaaaat ggatggactg
    36061 ggcaaaccag acgctcagta atgctcatcg cgggatcctg atgggattag tcagaacacc
    36121 accggaagag cgcgatcagg ccgccattga tgccagttgc aaagagtgcg acgccctgtt
    36181 tgccctgctc gatgcggaac tggcaaaagt aaaatggttc tctggcgacg agtttggtgt
    36241 gggcgatatc gctatcgcac cgttcatcta caatttgttt aacgttggcc tcacctggac
    36301 accgcgtcca aatctgcaac gctggtatca gcaactcact gaacgccccg cagtgcgcaa
    36361 agtggtgatg attcccgtta gctaattgcg tgggctaact ttaagtaatt ccccactgga
    36421 ctcgtcggtg agaacgtata aataaccgtc gggtccagtg cgaacatcac gaattcgctg
    36481 ccctctgtcc gttaaaatac ggccatcttc tgtcactttg tcgccgttga cgctcatcac
    36541 aatgacatct ttatctttca gcgcgccaat aaataatttt tgctgccact gggggaattt
    36601 atcgctgtta tagaaggcca tgccgctcac ggcgggcgaa tctttccagt aaaaaacagg
    36661 ttgctcggtc ccggcgacga tctccccttt cgcttccggt atcttaaagc ctgaatagtt
    36721 gattccccag gttgccagcg gccagccgta gtttttgcct ttttgcggga tattaatttc
    36781 atcaccaccg cgcgggccat gttcattcag ccatagtgca ttactccacg gattcatcgc
    36841 cattccttgc ggattacgaa tgccataaga ccagatctcg gcgcgcgcac cggattcctt
    36901 tataaaagga ttatcatccg ggatttcgcc ctggtcggtc agacgcacca gtttgccctg
    36961 taatttatcc agatcctgcg ccgtcgggcg ctgattgttt tcgcccagag caacaaaaag
    37021 ataaccttta ccgtcgaata ccagccgccc gccaaaatgg ttgccggtag acagttttgg
    37081 catctggcga aagacggtgc ggaagtcggt cacttttgag agatcatcac ttaagcggcc
    37141 ataacccaca gcagttccgg ctttgccatc atcgccaact tcggaataac ttaaccagat
    37201 gcggcgagac tgagcaaaat caggcgctaa aaccacgtcc agcaggccgc cctgcccgtg
    37261 cgcccaaacg tccggaactc cggaaagcgg cgcagataat ccttttcctg cttgccagtg
    37321 acgcaactcg ccgccgcgca gagtgattaa cataccgtga ttatcgggta aaaaggccag
    37381 tgcccaggga tggtcgagtt tgtcttgcag tacttcgaca tttaccgttg caggagccgc
    37441 ccagagagcg gaagaaagac aaataagggg cacaaggaaa aaggattgtc gatgcataac
    37501 gcctccggat tgactctggc ttaaagcgta gtcagtggag gagataacaa attcattttt
    37561 acaaaaactt aaacatgaag ggggagacgc tttctccccc ttagttttca ggccttctca
    37621 agcatggcgt gcttctgcag gctctggata ctcagcgtta agctcatcag acaattttca
    37681 agcttatcgg cgttgacggt aataacagtc gggcaatcat ggtgcccact catcaaacat
    37741 actgcggctg tcgctaatgc ttcttcagca tgatgaagag cactccactc ttcctgatcc
    37801 agatgaagat tcaaccgcag cgatttatcg tgcagttcgc gattcagttt aaaaaagtta
    37861 tctcgtagat gattgctttc gctgacggac atgtatcctt ttgccttcct cagttgcacg
    37921 tatgcagcga ggtcaacgcg tgcgtcgatc aaccggttca gcagatcgat tcgctgtctg
    37981 tcaacgaaca tacttcgatc ctcctctttc cagccttgtt gcaattaaat agtatggcta
    38041 ttttttgagc aaaatgctgt ccaggatcaa ttcttcacgc cgaattaaca aaatttacat
    38101 ttccttcgcc tccgccagcg aatgagattg cgatacaaat tttatccaga actctgtaga
    38161 atccctgccc tgacctttcc tgtaactgaa tattttttaa gctatgagca aagtaactcc
    38221 ccagccgaaa atcggctttg tttcccttgg ctgtccgaaa aaccttgtcg attcagagcg
    38281 tattctcacc gaactacgca ctgaaggtta tgacgtggta ccgagctatg acgatgcgga
    38341 catggtgatc gtcaacacct gcggctttat tgacagcgcg gtacaagaat cactggaagc
    38401 cattggtgaa gcgttgaatg aaaacggcaa ggtaattgtg accggttgtc tgggggcaaa
    38461 agaagatcag atccgcgaag tccacccgaa agtgctggaa atcaccgggc ctcatagcta
    38521 tgagcaggtt ctggagcacg ttcatcacta cgtgccaaaa ccgaaacaca acccattcct
    38581 gagcctggtg ccagaacaag gtgtgaaact gacgccgcgt cattatgcct atctgaaaat
    38641 ttcggaaggc tgtaatcacc gctgcacctt ctgcattatt ccgtctatgc gcggcgacct
    38701 ggtgagccgt ccgattggcg aagtgttaag tgaagcgaaa cgtctggtag atgcgggcgt
    38761 taaagagatt ctggtgatct cgcaggatac ttccgcctat ggcgttgatg ttaaacatcg
    38821 tactggcttc cacaacggcg agccggtaaa aaccagcatg gtcagcctgt gcgaacagtt
    38881 atcgaaactg gggatctgga cacgtctgca ctacgtttac ccttatccgc atgtggacga
    38941 cgtcatccca ctgatggcag aaggcaaaat cctgccgtat ctggacattc cgttgcagca
    39001 cgccagcccg cgcattctca aactgatgaa gcgtccgggt tctgtagatc gccaactggc
    39061 gcgcatcaaa cagtggcgcg aaatctgccc ggaactgacc ctacgctcaa cctttattgt
    39121 cggcttccct ggcgagacag aagaagattt ccagatgcta ctagacttcc tgaaagaagc
    39181 gcgtctggat cgcgttggct gctttaaata cagcccggtt gaaggtgcag acgccaatgc
    39241 cctgcctgac caggttccgg aagaagttaa agaagaacgc tggaaccgtt tcatgcagct
    39301 gcagcagcag atttccgccg agcgcctgca agagaaagtg ggccgtgaaa ttctggtgat
    39361 tatcgacgaa gtggacgaag aaggcgcgat tggtcgcagc atggcagatg caccggaaat
    39421 cgacggcgcg gtttatctca atggtgaaac caacgttaag ccgggtgata tcctgcgtgt
    39481 gaaagtcgag cacgccgatg agtacgattt gtggggtagc cgggtttaat taattcaggc
    39541 cgggcaaaat catttgtccg gcctgcgaat aatgattact gccagctaat ctgagccctg
    39601 ccgtttttct tactcttata cagccgctca tccgccagca ttttcaagcg ttcgacggtg
    39661 gtacattcat ttgaatgcgc tacaccgata ctggtactta aattaatgct tcccacctgg
    39721 aaatgaacga tttcttttag aatcgatagc aactcttcca gcgttccacc cgtataaatc
    39781 accgcaaatt cgtcaccgcc gaagcggtag attttacctt tatctaccgg gatctgtgat
    39841 ttaaggattt tcaccacttt catcaacact tcatcgccca gcaaatgacc gaaggtgtcg
    39901 ttaatgtttt tgaatttatc gacgtcaaac aggcacaaat aaccgccttt ttccggcagc
    39961 gcttttaaat cttcgtcaaa ggattttctg cccattgctt tggtcaggtc gtccaggaac
    40021 gccgagttat acatactgta gagttttttg acatcggcag ccgaaaagaa aagcccgcca
    40081 tccagtacgc gttcaaaatg gttattaatg atggcttctt tctccacaat accacgatta
    40141 atcactctgc gtaccagata gaggcaactg gtacccgata tcactgccac caatgcggca
    40201 aagaaaataa tcaatgtaca gacccgcaaa taaacatctt ttttatacgc aaatacgtac
    40261 aatccggtcg gcaaataggc gaagtgctcc atcgcttccg aaacggtgaa gatggcgttt
    40321 ttagcattat attcatcacc ggtcaccggt ttaccgttct taaatagaga gaacatcagg
    40381 ttctcgctgt ccatcgccag cgattttgac agtaaagaaa aacgctccag accaagcaaa
    40441 aatatcagac tacgattgtg agttaccgga tagaggtaat agacgaaggt tttaccgtca
    40501 aaagagataa accctgtatt tttgacatgt cgatacttca caacctgcat aatatctgca
    40561 caataatttt tctcgaactc ctttacctga atacattcgt cactggaaat cagataagga
    40621 ccgacatcgt acaaataaga gggggaaacc gagtcggcgt aagctgtgcg caaaccaacc
    40681 aacagctgtt cctccgtggg atttagcgtg ttgataagat caattgccac gctacgccga
    40741 tattcagcaa tatttgccac cgcaaaagca gtatcaataa cggactgttt caggctatac
    40801 acccgttcct ttaccatttg agtgtagatc ccgcaggcaa ctgctgaaat catgataaaa
    40861 atcagcagac tgactgtaag tacgaactta ttgattctgg acatacgtaa actactcttt
    40921 tactaatttt ccacttttat cccaggcgga gaatggcatg ggtttctggt acagataccc
    40981 ctgagcctga acacatccta atttaattaa ttcgcgcgca acgtcttcgc tctctacgcc
    41041 ttccgcaatc accgggatcc ggttatagcg tgccagatcg aaaataatgc gcacaatttt
    41101 tcccgcagta ccgaaaatat cgccggtaaa ttgcgagtcc acctttatgc tgtctggatt
    41161 aatttcataa aaacgtttca ggtttgacaa accggtgcca aagtcatcaa tcgatatccg
    41221 taccccttgt ttacgcagtt gttcaatatt gagataaaaa tgttcattaa ttcgctcgat
    41281 cacggtttca gtgatctcaa cacagaggct gttcgccttc aggccatttt ctttcatgat
    41341 ggtggtaaaa cgctcatgaa agtgcggttc gtttaactgc cagggcgaaa cattaatatg
    41401 cagtaagaaa tcatccttca gcgacgcacg ctgactaaaa tgtctgaatt cacccattgc
    41461 cgttttaata atctgataac ccagctcatt gataaacccg atatcttcag caatggtaat
    41521 gaacttcagc ggtgaaatga tcccccgctc cgcagaaacc caacggcaca acgcctccgc
    41581 gcccagaatg gcgcgggtgt taatatcaac gatcggttga tagacgagga atagttcgcc
    41641 aatttccatt gcatggcgaa tattacgcgc aatccagata ttctcttcta tttcgttgag
    41701 tttcgcgtca tcacagaaaa taaactcgga gaacatatgg tcacggatac tggaaatcgc
    41761 cagtttcagt ttttctgcgt aactccaggc gttttccccc tgcaaaggtt cacaaatggc
    41821 agacttatgg atgcgcagat tgcggtagcc aaagctgctg tcaaaatcgt ttaccatcgc
    41881 tattttacgg gtaaatgtct gtacattttc ccgacacaga agtaaataga gatcggcatt
    41941 gaatgtcacg atatcgcagc attgcgcata tttctcggca attcgctgac acaatttcct
    42001 gacgatttta ttgcgttcat caacaccaaa caccgcttcc acggcttcca gaccacacag
    42061 tttgatgatc cccacagcca gatactgatt attgttggac tcaataatcg ccatattttt
    42121 aataaaaccg ctttcctgat cgtaagagat cagtgagagc aatttatttg accgttcaat
    42181 ttcggcatct ttcatttccg cgatcgaatt tttgatgctg ataatctctt tcgcatcgcc
    42241 ttcgcgaatt ttcagagcct cgatttgatc cgttgagtcg ggcaaaaacg ccagcgtatt
    42301 aaccagctcg gtcatttcag aaaattgccg acggaacaca cgagtggtca tgaaatatac
    42361 caccagcaac acgataataa tgaaagcaaa cggtagataa aaaagtttat ctgatgcgtt
    42421 caataaatta ccgccaggat aataaaaata gatttcgaac aatgcattat ttggcactgc
    42481 tgatgtcttt gca
//
LOCUS       DAZFQD010000026        41822 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.026, whole genome shotgun sequence.
ACCESSION   DAZFQD010000026 DAZFQD010000000
VERSION     DAZFQD010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 41822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41822
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.026"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            303..389
                     /gene="leuL"
                     /locus_tag="ACHKTW_001485"
     CDS             303..389
                     /gene="leuL"
                     /locus_tag="ACHKTW_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HFO6462537.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     gene            482..2053
                     /gene="leuA"
                     /locus_tag="ACHKTW_001486"
     CDS             482..2053
                     /gene="leuA"
                     /locus_tag="ACHKTW_001486"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HFO6462538.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVANYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKAQHNENNKETV"
     gene            2053..3144
                     /gene="leuB"
                     /locus_tag="ACHKTW_001487"
     CDS             2053..3144
                     /gene="leuB"
                     /locus_tag="ACHKTW_001487"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HFO6462539.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLDAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDILCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAACAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            3147..4547
                     /gene="leuC"
                     /locus_tag="ACHKTW_001488"
     CDS             3147..4547
                     /gene="leuC"
                     /locus_tag="ACHKTW_001488"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HFO6462540.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            4558..5163
                     /gene="leuD"
                     /locus_tag="ACHKTW_001489"
     CDS             4558..5163
                     /gene="leuD"
                     /locus_tag="ACHKTW_001489"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HFO6462541.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDNAIAAYEAKQPAFMN"
     gene            complement(5212..6390)
                     /gene="setA"
                     /locus_tag="ACHKTW_001490"
     CDS             complement(5212..6390)
                     /gene="setA"
                     /locus_tag="ACHKTW_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetA"
                     /protein_id="HFO6462542.1"
                     /translation="MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREV
                     GAQPFWIGLFYTVNAIAGIGVSLWLAKRSDSQGDRRKLIIFCCLMAIGNALLFAFNRH
                     YLTLITCGVLLASLANTAMPQLFALAREYADNSAREVVMFSSVMRAQLSLAWVIGPPL
                     AFMLALNYGFTVMFSIAAGIFTLSLVLIAFMLPSVARVELPSENALSMQGGWQDSNVR
                     MLFVASTLMWTCNTMYIIDMPLWISSELGLPDKLAGFLMGTAAGLEIPAMILAGYYVK
                     RYGKRRMMVIAVAAGVLFYTGLIFFHSRMALMTLQLFNAVFIGIVAGIGMLWFQDLMP
                     GRAGAATTLFTNSISTGVILAGVIQGAIAQSWGHFAVYWVIAVISVVALFLTAKVKDV
                     "
     gene            complement(6492..6623)
                     /gene="sgrT"
                     /locus_tag="ACHKTW_001491"
     CDS             complement(6492..6623)
                     /gene="sgrT"
                     /locus_tag="ACHKTW_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046325 - negative regulation of glucose
                     import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HFO6462543.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEGLMQWDDRNSES"
     gene            6712..8367
                     /gene="sgrR"
                     /locus_tag="ACHKTW_001492"
     CDS             6712..8367
                     /gene="sgrR"
                     /locus_tag="ACHKTW_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HFO6462544.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIA
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPREWETLSNFASHPIGTGPYAVIRN
                     TTNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEEKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     regulatory      8401..8500
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            8531..9514
                     /gene="thiB"
                     /locus_tag="ACHKTW_001493"
     CDS             8531..9514
                     /gene="thiB"
                     /locus_tag="ACHKTW_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0030975 - thiamine binding [Evidence IEA];
                     GO_function: GO:0048502 - ABC-type thiamine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HFO6462545.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPKIKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDTFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            9490..11100
                     /gene="thiP"
                     /locus_tag="ACHKTW_001494"
     CDS             9490..11100
                     /gene="thiP"
                     /locus_tag="ACHKTW_001494"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HFO6462546.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGNWV
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALLQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            11084..11782
                     /gene="thiQ"
                     /locus_tag="ACHKTW_001495"
     CDS             11084..11782
                     /gene="thiQ"
                     /locus_tag="ACHKTW_001495"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HFO6462547.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDGVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTNELL
                     SGKASASALLGITG"
     gene            complement(11896..12660)
                     /gene="yabI"
                     /locus_tag="ACHKTW_001496"
     CDS             complement(11896..12660)
                     /gene="yabI"
                     /locus_tag="ACHKTW_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001148402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFO6462548.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGG"
     gene            complement(12746..13624)
                     /gene="araC"
                     /locus_tag="ACHKTW_001497"
     CDS             complement(12746..13624)
                     /gene="araC"
                     /locus_tag="ACHKTW_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HFO6462549.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            13963..15663
                     /gene="araB"
                     /locus_tag="ACHKTW_001498"
     CDS             13963..15663
                     /gene="araB"
                     /locus_tag="ACHKTW_001498"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HFO6462550.1"
                     /translation="MAIAIGLDFGSDSVRALAVDCASGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKTVLAELSVEQRAAVVGIGVDTTGSTPAPIDANGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDTAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKAQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATDAPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVATL
                     "
     gene            15674..17176
                     /gene="araA"
                     /locus_tag="ACHKTW_001499"
     CDS             15674..17176
                     /gene="araA"
                     /locus_tag="ACHKTW_001499"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HFO6462551.1"
                     /translation="MTIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PCKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLAVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            17345..18040
                     /gene="araD"
                     /locus_tag="ACHKTW_001500"
     CDS             17345..18040
                     /gene="araD"
                     /locus_tag="ACHKTW_001500"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFO6462552.1"
                     /translation="MLEDLKRQVLEANLALPKHNLVTLTWGNISAVDRERGVFVIKPS
                     GVDYSVMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQSGQSIPAIGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            18115..20466
                     /gene="polB"
                     /locus_tag="ACHKTW_001501"
     CDS             18115..20466
                     /gene="polB"
                     /locus_tag="ACHKTW_001501"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HFO6462553.1"
                     /translation="MAQAGFILTRRWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IPADQVPRAQHILQGEQGFRLTPLALKDFHRQPVYGLYCRAHSQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGTIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASSLDFELEYVASRPLLLEKLNAWFANHDPD
                     VIIGWNVVQFDLRMLQKHAERYRLPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDVIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRETIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            20631..23537
                     /gene="rapA"
                     /locus_tag="ACHKTW_001502"
     CDS             20631..23537
                     /gene="rapA"
                     /locus_tag="ACHKTW_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HFO6462554.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKNCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            23549..24208
                     /gene="rluA"
                     /locus_tag="ACHKTW_001503"
     CDS             23549..24208
                     /gene="rluA"
                     /locus_tag="ACHKTW_001503"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HFO6462555.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(24325..25140)
                     /gene="djlA"
                     /locus_tag="ACHKTW_001504"
     CDS             complement(24325..25140)
                     /gene="djlA"
                     /locus_tag="ACHKTW_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HFO6462556.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            25395..27749
                     /gene="lptD"
                     /locus_tag="ACHKTW_001505"
     CDS             25395..27749
                     /gene="lptD"
                     /locus_tag="ACHKTW_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HFO6462557.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKLLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYRYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASWPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            27802..29088
                     /gene="surA"
                     /locus_tag="ACHKTW_001506"
     CDS             27802..29088
                     /gene="surA"
                     /locus_tag="ACHKTW_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0042277 - peptide binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA];
                     GO_process: GO:0050821 - protein stabilization [Evidence
                     IEA];
                     GO_process: GO:0060274 - maintenance of stationary phase
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HFO6462558.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            29088..30077
                     /gene="pdxA"
                     /locus_tag="ACHKTW_001507"
     CDS             29088..30077
                     /gene="pdxA"
                     /locus_tag="ACHKTW_001507"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HFO6462559.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADTTLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTTGTLTLLPVALRESVTAGQLGVENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDAGIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLDELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGKADVGSFITALNLAIKMIVNTQ"
     gene            30074..30895
                     /gene="rsmA"
                     /locus_tag="ACHKTW_001508"
     CDS             30074..30895
                     /gene="rsmA"
                     /locus_tag="ACHKTW_001508"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFO6462560.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            30898..31275
                     /gene="apaG"
                     /locus_tag="ACHKTW_001509"
     CDS             30898..31275
                     /gene="apaG"
                     /locus_tag="ACHKTW_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HFO6462561.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            31282..32130
                     /gene="apaH"
                     /locus_tag="ACHKTW_001510"
     CDS             31282..32130
                     /gene="apaH"
                     /locus_tag="ACHKTW_001510"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HFO6462562.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGTLTCLRWEDKQYFVQPSNRHKDLGE
                     GEAVAS"
     gene            32155..32754
                     /locus_tag="ACHKTW_001511"
     CDS             32155..32754
                     /locus_tag="ACHKTW_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000796364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4291 domain-containing protein"
                     /protein_id="HFO6462563.1"
                     /translation="MADYFEIRADYNQHTITVYQAYNDAIADVAVRDGRFGASFSFNR
                     MTWIKPSFMWMMERSNWGLKKDQQHILAIRIKRTFFDTLLEQAVLTTPEAHVYPHAGI
                     WETLFAQANVYVQWDPERSINGKKLEHRSLQLGISRNLISQFNEDAIVAIDDLTPLVR
                     KCHNLLINGKTTQAKSFLPPEKIYPVSAAARKALGMKNS"
     gene            32790..33257
                     /locus_tag="ACHKTW_001512"
     CDS             32790..33257
                     /locus_tag="ACHKTW_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6462564.1"
                     /translation="MRQINSGLNTREIILAMIDEADEPPFAEQRTWHRRRIAFLCDCI
                     RVAIDYGFFCSVSQQSIMNITRMAEDVWITESKTTQLIDIWQQLKQLYPPETIPYVHK
                     LPTKELLELLLSSPDDLRIIEIPDIIGDVFDDQTWIRLFKKHFADIIQLRCDV"
     gene            complement(33356..33535)
                     /locus_tag="ACHKTW_001513"
     CDS             complement(33356..33535)
                     /locus_tag="ACHKTW_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system CcdA family
                     antitoxin"
                     /protein_id="HFO6462565.1"
                     /translation="MNDKTFDFTSERDFVSRAHCVGLNLQATLQWNEVNEDAISALNE
                     FAEETGCFSDEYRKF"
     gene            complement(33556..34035)
                     /gene="folA"
                     /locus_tag="ACHKTW_001514"
     CDS             complement(33556..34035)
                     /gene="folA"
                     /locus_tag="ACHKTW_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HFO6462566.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(34228..36090)
                     /gene="kefC"
                     /locus_tag="ACHKTW_001515"
     CDS             complement(34228..36090)
                     /gene="kefC"
                     /locus_tag="ACHKTW_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HFO6462567.1"
                     /translation="MDSHTLVQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLATSSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLLENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWAKSLTLAVALSMAVTPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(36083..36613)
                     /gene="kefF"
                     /locus_tag="ACHKTW_001516"
     CDS             complement(36083..36613)
                     /gene="kefF"
                     /locus_tag="ACHKTW_001516"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HFO6462568.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVFSHGWAYGHGGTALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(36721..38052)
                     /gene="yaaU"
                     /locus_tag="ACHKTW_001517"
     CDS             complement(36721..38052)
                     /gene="yaaU"
                     /locus_tag="ACHKTW_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO6462569.1"
                     /translation="MQPSRNFDDLKFSSIHRRILLWGSGGPFLDGYVLVMIGVALEQL
                     TPALKLDADWIGLLGAGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATMF
                     VSSPVELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADL
                     VGYWLYDVEGGWRWMLGSAAIPCLLILIGRFELPESPRWLLRKGRVKECEEMMIKLFG
                     EPVAFDEEQPQQTRFRDLFNRRHFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGLLGLG
                     VGKNAALGNVVISLFFMLGCIPPMLWLNTAGRRPLLIGSFAMMTLALAVLGLIPDMGI
                     WLVVMAFAVYAFFSGGPGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTWALPI
                     FINNYGISNTMLMGAGISLFGLLISVAFAPETRGMSLAQTSNMTIRGQRMG"
     gene            complement(38110..38397)
                     /gene="fixX"
                     /locus_tag="ACHKTW_001518"
     CDS             complement(38110..38397)
                     /gene="fixX"
                     /locus_tag="ACHKTW_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HFO6462570.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQVLELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(38394..39680)
                     /gene="fixC"
                     /locus_tag="ACHKTW_001519"
     CDS             complement(38394..39680)
                     /gene="fixC"
                     /locus_tag="ACHKTW_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFO6462571.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFADSAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVILAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGYPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(39731..40672)
                     /gene="fixB"
                     /locus_tag="ACHKTW_001520"
     CDS             complement(39731..40672)
                     /gene="fixB"
                     /locus_tag="ACHKTW_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HFO6462572.1"
                     /translation="MNTFSQVWVFSDTPSRLPELMNGAQALANQINTFVLNDADGVQA
                     IQLGANHVWKLNGKPDDRMIEDYAGVMADTIRQHGADGLVLLPNTRRGKLLAAKLGYR
                     LKAAVSNDASTVSVQDGKATVKHMVYGGLAIGEERIATPYAVLTISSGTFDAAQPDAS
                     RTGETHTVEWQAPAVAITRTATQARQSNSVDLDKARLVVSVGRGIGSKENIALAEQLC
                     KAIGAELACSRPVAENEKWMEHERYVGISNLMLKPELYLAVGISGQIQHMVGANASQT
                     IFAINKDKNAPIFQYADYGIVGDAVKILPALTAALAR"
     gene            complement(40687..41457)
                     /gene="fixA"
                     /locus_tag="ACHKTW_001521"
     CDS             complement(40687..41457)
                     /gene="fixA"
                     /locus_tag="ACHKTW_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HFO6462573.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAVDA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
BASE COUNT        10177 a        11442 c        10885 g         9318 t
ORIGIN      
        1 aaaaatcgca aaacatataa ttcaatacaa atcatcagga taggttttgc aacgcgtgca
       61 ttttgtcccc tttttcctcg ttgattagat gcaaaaattt atgctgaaat atgtcaaccg
      121 atgaaaagcg tcggtagtta agcagaaatt aatatcgctt actttaacca ccgcagcaca
      181 attagctaat tttacggatg cagaactcac gctggcggga cgtttttatt gcgtcagggt
      241 tgacatccgt ttttgtatcc agtaactcta aaagcatatc gcattcatct ggagctgatt
      301 taatgactca catcgttcgc tttatcggtc tactactact aaacgcatct tctttgcgcg
      361 gtagacgagt gagcggcatc cagcattaag ccagcacgca gtcaaacaaa aaacccgcgc
      421 cattgcgcgg gtttttttat gcccgaagcg aggcgctcta aaagagacaa ggacccaaac
      481 catgagccag caagtcatta ttttcgatac cacattgcgc gacggtgaac aggcgttaca
      541 ggcaagcttg agtgtgaaag aaaaactgca aattgcgctg gcccttgagc gtatgggtgt
      601 tgacgtgatg gaagtcggtt tccccgtctc ttcgccgggc gattttgaat cggtgcaaac
      661 catcgcccgc caggttaaaa acagccgcgt atgtgcgtta gctcgctgcg tggaaaaaga
      721 tatcgacgtg gcggccgaat ccctgaaagt cgccgaagcc ttccgtattc atacctttat
      781 tgctacctcc ccaatgcaca tcgccaccaa gctgcgcagc accctggacg aggtgatcga
      841 acgcgctatc tatatggtga aacgcgcccg taattacacc gatgatgttg aattttcttg
      901 cgaagatgcc gggcgtacac ccattgccga tctggcgcga gtggtcgaag cggcgattaa
      961 tgccggtgcc accaccatca acattccgga caccgtgggc tacaccatgc cgtttgagtt
     1021 cgccggaatc atcagcggcc tgtatgaacg cgtacctaac atcgacaaag ccattatttc
     1081 cgtacatacc cacgacgatt taggcctggc ggtcggcaac tcactggcgg cggtacatgc
     1141 cggtgcacgc caggtggaag gtgcaatgaa cgggatcggc gagcgtgccg gtaactgttc
     1201 gctggaagaa gtcatcatgg cgattaaagt tcgtaaggat attctcaacg tccacaccgc
     1261 cattaatcac caggagatat ggcgcaccag ccagttagtt agccagattt gtaatatgcc
     1321 gatcccggca aacaaagcca ttgttggcag cggcgcattc gctcactcct ccggtatcca
     1381 ccaggatggc gtgctgaaaa accgcgaaaa ctacgaaatc atgacaccag aatctattgg
     1441 tctgaaccaa atccagctga atctgacctc tcgttcgggg cgtgcggcgg tgaaacatcg
     1501 catggatgag atggggtata aagaaagtga atataattta gacaatttgt acgatgcttt
     1561 cctgaagctg gcggacaaaa aaggtcaggt gtttgattac gatctggagg cgctggcctt
     1621 catcggtaag cagcaagaag agccggagca tttccgtctg gattacttca gcgtgcagtc
     1681 tggctctaac gatatcgcca ccgccgccgt caaactggcc tgtggcgaag aagtcaaagc
     1741 agaagccgcc aacggtaacg gtccggtcga tgccgtctat caggcaatta accgcatcac
     1801 tgactataac gtcgaactgg tgaaatacag cctgaccgcc aaaggtcacg gtaaagatgc
     1861 gctgggtcag gtggatattg tcgccaacta caacggtcgc cgcttccacg gcgtcggcct
     1921 ggccaccgat attgtcgagt cctccgccaa agccatggtg cacgtactga acaatatctg
     1981 gcgtgccgca gaagtcgaaa aagagttgca acgcaaagct caacacaacg aaaacaacaa
     2041 ggaaaccgtg tgatgtcgaa gaattaccat attgccgtat tgccggggga cggtattggt
     2101 ccggaagtga tgacccaggc gctgaaagtg ctggatgccg tgcgcaaccg ctttgcgatg
     2161 cgcatcacca ccagccatta cgatgtaggc ggcgcagcca ttgataacca cgggcaacca
     2221 ctgccgcctg cgacggttga aggttgtgag caagccgatg ccgtgctgtt tggctcggta
     2281 ggcggtccga aatgggaaca tttaccacca gaccagcaac cagaacgcgg cgcgctgttg
     2341 cctttgcgta agcacttcaa attattcagc aacctgcgcc cggcaaaact gtatcagggg
     2401 ctggaagcat tctgtccgct gcgtgcagac attgccgcta acggcttcga catcctgtgc
     2461 gtgcgcgaac tgaccggcgg catctatttc ggtcagccaa aaggccgcga aggtagcgga
     2521 caatatgaaa aagcgtttga taccgaggtg tatcaccgtt ttgagatcga gcgtattgcc
     2581 cgcatcgcgt ttgaatctgc tcgtaaacgt cgccacaaag tcacctcaat cgacaaagct
     2641 aacgtgctgc aatcctctat tttatggcgt gaaatcgtca acgagatcgc cacggaatac
     2701 ccggatgtcg aactggcgca tatgtacatc gacaacgcca ccatgcagct gattaaagat
     2761 ccatcacagt ttgacgttct gctgtgctcc aacctgtttg gcgacattct gtctgacgag
     2821 tgcgcaatga tcactggctc gatggggatg ttgccttccg ccagcctgaa cgagcaaggt
     2881 tttggtctgt atgaaccggc aggcggctca gcaccagata tcgcaggcaa aaacatcgcc
     2941 aacccgattg cgcaaattct gtcgctggca ctgctgctgc gctacagcct ggatgccgat
     3001 gatgcggctt gcgccattga acgcgccatt aaccgcgcat tagaagaagg cattcgcacc
     3061 ggggatttag cccgtggcgc tgccgccgtt agtaccgatg aaatgggcga tatcattgcc
     3121 cgctatgtgg cagaaggggt gtaatcatgg ctaaaacgtt atacgaaaaa ttattcgacg
     3181 ctcacgtagt gtacgaagcc gaaaacgaaa ccccgctgtt atatatcgac cgccacctgg
     3241 tgcatgaagt gacctcaccg caggcgtttg atggtctgcg cgcccacggt cgcccggtac
     3301 gtcagccggg caaaaccttc gccactatgg atcacaacgt ctctacccag accaaagaca
     3361 ttaatgcctg tggtgaaatg gcgcgcatcc agatgcagga gctgattaaa aactgcaaag
     3421 aatttggcgt cgagctgtat gacctgaatc acccgtatca ggggatcgtc cacgtaatgg
     3481 ggccggaaca gggcgtcacc ttgccgggga tgaccattgt ctgcggcgac tcgcataccg
     3541 ccacccacgg cgcgtttggc gcactggcct ttggtatcgg cacttccgaa gttgaacacg
     3601 tactggcaac acaaaccctg aaacagggcc gtgcgaagac catgaaaatt gaagtccagg
     3661 gcaaagccgc gccgggcatt acagcaaaag atatcgtgct ggcaattatc ggtaaaactg
     3721 gcagcgcagg cggcaccggg catgtggtgg agttttgcgg cgaagcaatt cgtgatttaa
     3781 gcatggaagg tcgtatgacc ctgtgcaata tggcaatcga aatgggcgca aaagccggtc
     3841 tggttgcacc ggacgaaacc acctttaact atgtcaaagg ccgtttgcat gcgccgaaag
     3901 gcaaagattt cgacgacgcc gttgcctact ggaaaaccct gcaaaccgac gaaggcgcaa
     3961 ctttcgatac cgttgtcact ctgcaagcag aagaaatttc accgcaggtc acctggggca
     4021 ccaatcctgg ccaggtgatt tccgtgaacg acaatattcc cgatccggct tcgtttgccg
     4081 atccggttga acgcgcgtcg gcagaaaaag cgctggccta tatggggctg aaaccgggca
     4141 ttccgctgac cgaagtggct atcgacaaag tgtttatcgg ttcctgtacc aactcgcgca
     4201 ttgaagattt acgcgcggca gcggagatcg ccaaagggcg aaaagtcgcg ccaggcgtgc
     4261 aggcactggt ggttcccggc tctggcccgg taaaagccca ggcggaagcg gaaggtctgg
     4321 ataaaatctt tattgaagcc ggttttgaat ggcgcttgcc tggctgctca atgtgtctgg
     4381 cgatgaacaa cgaccgtctg aatccgggcg aacgttgtgc atccaccagc aaccgtaact
     4441 ttgaaggccg ccaggggcgc ggcgggcgca cgcatctggt cagcccggca atggctgccg
     4501 ctgctgctgt gaccggacat ttcgccgaca ttcgcaacat taaataagga gcacaccatg
     4561 gcagagaaat ttatcaaaca cacaggcctg gtggttccgc tggatgccgc caatgtcgat
     4621 accgatgcaa tcatcccgaa acagtttttg cagaaagtga cccgtacggg ttttggcgcg
     4681 catctgttta acgactggcg ttttctcgat gaaaaaggcc aacagccaaa cccggacttc
     4741 gtgctgaact tcccgcagta tcagggcgct tccattttgc tggcacgaga aaacttcggc
     4801 tgtggctcct cgcgtgagca cgcgccctgg gcattgaccg actacggttt taaagtggtg
     4861 attgcgccga gttttgctga catcttctac ggcaatagct ttaacaacca gctgctgccg
     4921 gtgaaattaa gcgatgcaga agtggacgaa ctgtttgcgc tggtgaaagc caatccgggg
     4981 atccatttcg acgtggatct ggaagcgcaa gaggtgaaag cgggagagaa aacctatcgc
     5041 tttaccatcg atgccttccg ccgccactgc atgatgaacg gtctggacag tattgggctt
     5101 accttgcagc acgacaacgc cattgccgct tatgaagcaa aacaacctgc gtttatgaat
     5161 taatcccctt gcccggtcaa atgaccgggc tttccgctat cgtccacgtc atcaaacgtc
     5221 tttaaccttt gcggttaaaa ataatgcgac aacagaaata accgcaatta cccagtagac
     5281 agcaaagtgc ccccaacttt gtgcaattgc tccctgaata acgccagcca gaattacccc
     5341 ggtagaaata ctgttagtaa ataaggtggt agctgccccc gctcttccag gcattaaatc
     5401 ctgaaaccat agcatcccaa tacccgcaac aatgccgata aatacagcgt taaaaagttg
     5461 cagcgtcatc aacgccatac ggctatgaaa gaaaatcaat ccggtgtaaa acagtactcc
     5521 tgccgccact gctatgacca tcattcgccg cttaccataa cgtttgacat agtagccagc
     5581 cagaatcatt gctggtattt ccagtccagc tgccgtcccc atcaggaaac ccgccagttt
     5641 gtctggcaat cctaactcgc tactgatcca caacggcata tcaataatgt acatggtgtt
     5701 gcaggtccac attaacgtcg aggcgacaaa taacatccgt acgttactat cctgccagcc
     5761 accttgcatt gataaagcat tttccgacgg cagttctacc cgcgccacag acggaagcat
     5821 aaatgcaatc aataccagac tgagtgtgaa tatcccggcg gcaatcgaaa acatcaccgt
     5881 aaagccgtaa ttcaacgcca gcataaaggc caacggtgga ccgataaccc atgccagaga
     5941 aagctgcgca cgcatcaccg agctaaacat caccacttct cgcgccgagt tatccgcata
     6001 ttcccgcgcc agagcaaata actgtggcat tgccgtattg gccagagatg ccagaagcac
     6061 accacaggtg ataagcgtca gataatgacg attaaatgca aacaatagcg cattgccgat
     6121 agccatcaaa cagcaaaata taatcagttt tcgccgatcg ccctgactgt cagaacgttt
     6181 tgccaaccag aggcttacgc cgatcccagc aatagcattc accgtataaa agagtccgat
     6241 ccagaaaggt tgcgcgccaa cctcacgact cagaaataag ctcaatgtag gagcctgtag
     6301 cgccccggcc acccccatca taaaagcgac cagcataaat gccgcgtaaa caccgttcat
     6361 acggcgagcc atcgtcatta tccagatcat acgttccctt tttagcgcgg cgagaataaa
     6421 aaaaaccagc aggtataatc tgctggcggg tgattttaca ccaatactca gtcacacatg
     6481 atgcaggcaa gtcaactttc agaattgcgg tcatcccact gcatcagtcc ttcaagcatg
     6541 gttaatcgtt gtgggacgct taaccaacgc aaccagcaca acttcgctgt cgcggtaaaa
     6601 tagtgctgat aaaactgacg catggggcac ctccttgctt catcgttagg aattattgac
     6661 ttaatatagg gaaaataaaa ttgctgtctt ttgcgcagga gttccccttt tatgccatct
     6721 gctcgtctgc aacaacagtt catccgcctg tggcaatgct gcgagggtaa atcgcaggac
     6781 acaacgctca acgaactggc agcgttattg agctgctcgc gtcgtcatat gcgcaccctg
     6841 ctcaacacca tgcaggatcg cggctggctg acgtgggaag cggaagtcgg gcgtggtaaa
     6901 cgctcgcgtc tgacattcct ctataccggg ctggcgcttc agcaacagcg ggcggaagac
     6961 ctgctggagc aggatcgtat cgaccaacta gtgcagttgg ttggcgacaa agcgactgtg
     7021 cggcaaatgc tggtttctca tctgggccgc agcttccgcc aggggcggca catcctgcgc
     7081 gtgctctact atcgtccgtt gcgtaatctg ctacctggca gcgcattgcg ccgttccgaa
     7141 acccatattg cccggcaaat cttcagttcg ctaacgcgca taaatgagga aaatggggaa
     7201 ctggaagcag acatcgccca ccactggcag caaatatcac cgcttcactg gcgtttcttt
     7261 ttgcgtccag gagtccattt tcatcatggt cgtgaactgg aaatggacga cgtgatcgcc
     7321 tctttaaaac gaatcaatac gctgccgctc tattcgcata ttgcggacat tgtgtcgccg
     7381 acgccctgga cgctggatat ccatctcacg caaccggacc gctggttacc gttactgctg
     7441 ggacaagttc cggcgatgat cctgccgcgc gaatgggaaa ccctcagtaa ctttgccagc
     7501 catcccatcg gcaccggtcc gtatgcggtg atccgcaaca ccaccaatca actgaaaatt
     7561 caggcattcg atgacttctt cggttaccgg gcattaatcg atgaagttaa cgtctgggtt
     7621 ctgccggaaa ttgccgacga gccagctgga ggtctgatgc taaaaggtcc acagggtgag
     7681 gaaaaagaga ttgaaagccg cctggaggaa ggttgctact atttactgtt cgacagccgc
     7741 acccatcgcg gggcgaatca gcaagtcagg gactgggtaa gctatgtgct ttctccaact
     7801 aatctggtct atttcgctga ggaacagtac cagcaactgt ggttcccggc ttatggactg
     7861 ctcccccgtt ggcaccatgc ccgcaccata aagagcgaaa aaccggctgg cctggaaagc
     7921 ctcaccctga ccttttatca ggatcacagt gagcatcggg tgattgccgg gatcatgcag
     7981 cagattctgg caagtcacca ggtcacgctg gaaatcaaag agatcagcta cgatcagtgg
     8041 catgaaggag agatcgagag cgatatctgg cttaacagcg ccaactttac gctgccgctg
     8101 gatttttcgc tgttcgcgca cttgtgcgag gtgccgctgc tccaacactg tattccgatc
     8161 gactggcaag ccgacgccgc ccgctggcgc aatggcgaaa tgaacctggc gaactggtgc
     8221 cagcaactgg tcgccagcaa agcaatggtg ccgcttatcc accactggct gatcattcag
     8281 gggcaacgca gtatgcgcgg cctgcgcatg aacaccctcg gctggttcga ttttaaatca
     8341 gcgtggtttg cgccaccgga tccatgattg ctggtagata acaaaatcac tacactaacg
     8401 ccgttctcaa cggggtgcca cgcaaacgcg tgcgctgaga aaatacccgt cgaacctgat
     8461 ccggataacg ccggcgaagg gatttgaggc tccttctcaa gtcctttgcc actctttttt
     8521 gaggtgcaaa gtgttaaaaa aatgtcttcc cctgctgttg ctgtgcacag cgcccgtttt
     8581 cgctaaaccc gttctgactg tttataccta cgattccttc gccgccgact gggggcctgg
     8641 tccaaagatc aaaaaagctt ttgaagccga ctgtaactgc gaactgaaac tggtggcgct
     8701 ggaagatggc gtttcgcttc tcaaccgtct acggatggaa ggcaaaaata gtaaagccga
     8761 tgtggtgctg gggctggata acaatctgtt agacgccgcc agtaaaaccg gactatttgc
     8821 caaaagcggt gtggcagcgg atgccgttaa cgttcccggc ggctggaata atgacacttt
     8881 cgtcccgttt gattacggct atttcgcctt cgtctatgac aagaacaaac tgaaaaaccc
     8941 gccgcaaagc ctgaaagaat tggttgagag cgatcagaac tggcgggtga tttatcagga
     9001 tccgcgcacc agtacgccgg ggctgggcct gctgttgtgg atgcaaaaag tctatggcga
     9061 tgacgcccca caagcctggc aaaaactggc gaagaaaacc gtcacggtca ccaaaggctg
     9121 gagcgaagcc tacggcctgt ttttaaaagg tgaaagcgat ctggtactga gttacaccac
     9181 ctctccggct tatcacattc tcgaagagaa aaaagataac tacgccgccg cgaactttag
     9241 cgaaggtcac tatctgcagg tggaagtcgc cgcccgcacc gctgccagca agcagccgga
     9301 gctggcgcaa aaattcctcc agtttatggt ttctccggct tttcagaatg cgatcccaac
     9361 cggtaactgg atgtatccgg tggcaaatgt cacgctgcct gccgggtttg aacaattgac
     9421 caaaccggcc accacgctgg agttcacacc agctgaagtg gcggcacaac gtcaggcatg
     9481 gattagcgaa tggcaacgcg ccgtcagccg ttaattcccg gctggttaat tccaggtgta
     9541 agcgccgcca cgctggtggt agcggttgcg ctcgcggcgt ttctcgccct gtggtggaac
     9601 gcgccgcagg gtaactgggt ggcagtctgg caggacagct acctgtggca tgtggtgcgc
     9661 ttctccttct ggcaggcgtt tctctcggcg ctactctctg tcgtacccgc gatattcctc
     9721 gcccgcgccc tctatcgcag gcgctttccg ggtcggctgg cgctgttgcg tctgtgcgca
     9781 atgaccttga tcctcccggt gctagttgct gttttcggca ttcttagcgt ctatggtcgt
     9841 cagggctggc tggcatcgct ctgccaatcg ctcggtctgg agtggacctt ttcgccctac
     9901 ggcctgcaag gcattttgct ggcgcacgta ttttttaatc tgccgatggc gagccgctta
     9961 ttactccagg cactggaaaa cattcccggc gaacaacgtc agcttgccgc ccagcttggg
    10021 atgcgcggct ggcatttttt ccgcttcgtc gaatggccgt ggctacggcg acaaatcccg
    10081 ccggttgccg cgcttatctt tatgctctgt ttcgccagct tcgccaccgt gctatcgctg
    10141 ggcggcggtc cgcaggcaac tactatcgag ctggctattt atcaggcgct gagttacgac
    10201 tacgatcctg cccgcgcggc gatgctggcg ctgctccaga tggtgtgttg ccttgggctg
    10261 gtgctgttga gtcagcgact aagtaaggcc attgcgcccg gtaccacgct gctgcaaggc
    10321 tggcgcgatc cggacgatcg tctgcatagc cgcatttgcg acacggtgtt aatagtgctg
    10381 gcgctgctgc tgttgctgcc accgttgctg gcggtgatcg tcgatggggt aaatcgccag
    10441 ttgccggaag tgctggcaca accggtgctg tggcaggcgc tgtggacctc gttgcgtatt
    10501 gcgctggcgg caggtgtatt gtgcgtagtg ctgaccatga tgctgctatg gagcagtcgc
    10561 gaacttcggg cgcggcagaa aatgctggcg ggtcaggcgc tggagatgag cggcatgttg
    10621 atcctcgcca tgccggggat tgtgctggct accggcttct ttttactgct caacaacacc
    10681 attggcctgc cgcaatctgc tgacggcatt gtgattttca ccaatgcgtt aatggcgatc
    10741 ccttatgcgc tgaaagtact ggaaaacccg atgcgcgata tcaccgcccg ctacagtatg
    10801 ttgtgtcagt cgctgggcat tgaaggctgg tcgcgcttaa aagtggtcga gctgcgcgcc
    10861 ctgaaacgtc cactggcgca ggcgctggct tttgcctgcg tgctgtcgat tggtgatttt
    10921 ggcgtagtgg cgttgttcgg taacgatgat ttccgtaccc tgccgtttta tctctaccag
    10981 caaattggct cctatcgcag ccaggacggt gcggtcaccg cgttaattct gctactactc
    11041 tgttttctgc tgtttaccgt gattgaaaaa ctaccggggc gaaatgttaa aactgactga
    11101 tatcacctgg ctttatcacc atttgccgat gcgttttagc ttaacggtag aacgcggcga
    11161 gcaggtggca atcctcgggc caagcggcgc gggtaaaagt accctgctga atttgatcgc
    11221 cggttttctg acgccagcca gcggttcgct gactatcgac ggcgttgatc acaccacgac
    11281 gccgccgtca cgccgtccgg tgtcgatgct gtttcaggaa aacaacctgt tcagtcacct
    11341 gacggtcgcg cagaacatcg ggctggggct aaatccggga ttgaaactga acgcggcaca
    11401 gcaggagaaa atgcacgcta tcgcccgcca gatggggatt gataatttaa tggcgcggtt
    11461 acctggcgag ctttccggcg gtcagcgaca gcgcgtggcg ttagcgcgtt gtctggtgcg
    11521 cgaacagccg attttattgc tcgatgaacc gttctctgcg ctcgatccgg cgttacgtca
    11581 ggagatgctg acgctggtga gcacgagctg ccagcagcaa aaaatgacgc tattgatggt
    11641 gtcgcacagt gtggaagatg cggcgcggat cgccacacgc tcggtggtgg tcgccgacgg
    11701 gcgcatcgcc tggcagggta agaccaatga gttgttgagc ggtaaggcga gtgcttcggc
    11761 actattgggg attacgggtt agtatgcact gtcggatgcg gcgtgaacgc cttatccgac
    11821 ctacgggtag tgcgttttgt aggcctgata agacgcggtc agcgtcgcat caggcaaccc
    11881 gcacaagact atctcctaac ccccaaccac tttacgcaaa atatcgatat acaccggcat
    11941 caacgggtgg cgaattaaca ccaccagcgc caccacgccg atggcagaaa ttaacggcgt
    12001 cagccacaac aaacgaccac gggacaaata atgactcaaa cggtcagtcg ctttaccact
    12061 gcgccataac cgccagcaca gccagccacc aacccacaaa aacaccgctg ttgccagcag
    12121 caaccattta aactcaccgc tctgcattcc ggctggaata tcgatcgccg cgcccgccag
    12181 aatccctggc aggaagtaaa acggtggcca cagcaggcag ccgataatat tcggcgtaat
    12241 gaattttgcc accggcagat ccagcattcc cgccaccatt ggcaccagcg gacgcgtcgg
    12301 gccaacaaaa cgaccgacca gaatggtgaa catgctgtgt tgatgcaacg catgttcagt
    12361 tttatcgagt aatgctttgt ttttcttcag aaatgaccag cgatgcaacg gctttttaaa
    12421 acgccagccc agccagaagg agatccagtc gcccagcagg caaccgacga tccctgccag
    12481 ccaggcgtgc cagaaactta actcgccgct gccaatcagc gctcccagcc ccgccatcag
    12541 caccgtaccg ggtagaatca aaccgaccag cgccagcgac tccagaaagg ccaccaacac
    12601 caccgccatc aatgaataca cggtagattg ggtaataaag tgttccagca atgcttgcat
    12661 aatgtgcctg tcaaatggac gaagcaggga ttctgcaaac cctatgctac tccgtcaagc
    12721 cgtcaattgt ctgattcgtt accaattatg acaacttgac ggctacatca ttcacttttt
    12781 cttcacaacc ggctcggaac tcgctcgggc tggccccggt gcatttttta aatacccgcg
    12841 agaaatagag ttgatcgtca aaaccaacat tgcgaccgac ggtggcgata ggcatccggg
    12901 tggtgctcaa aagcagcttc gcctggctga tacgttggtc ctcgcgccag cttaagacgc
    12961 taatccctaa ctgctggcgg aaaagatgtg acagacgcga cggtgacaag caaacatgct
    13021 gtgcgacgct ggcgatatca aaattgctgt ctgccaggtg atcgctgatg tactgacaag
    13081 cctcgcgtac ccgattatcc atcggtggat ggagcgactc gttaatcgct tccatgcgcc
    13141 gcagtaacaa ttgctcaagc agatttatcg ccagcagctc cgaatagcgc ccttcccctt
    13201 gcccggcgtt aatgatttgc ccaaacaggt cgctgaaatg cggctggtgc gcttcatccg
    13261 ggcgaaagaa acccgtattg gcaaatattg acggccagtt aagccattca tgccagtagg
    13321 cgcgcggacg aaagtaaacc cactggtgat accattcgcg agcctccgga tgacgaccgt
    13381 agtgatgaat ctctcctggc gggaacagca aaatatcacc cggtcggcat acaaattctc
    13441 gtccctgatt tttcaccacc ccctgaccgc gaatggtgag attgagaata taacctttca
    13501 ttcccagcgg tcggtcgata aaaaaatcga gataaccgtt ggcctcaatc ggcgttaaac
    13561 ccgccaccag atgggcatta aacgagtatc ccggcagcag gggatcattt tgcgcttcag
    13621 ccatactttt catactcccg ccattcagag aagaaaccaa ttgtccatat tgcatcagac
    13681 attgccgtca ctgcgtcttt tactggctct tctcgctaac ccaaccggta accccgctta
    13741 ttaaaagcat tctgtaacaa agcgggacca aagccatgac aaaaacgcgt aacaaaagtg
    13801 tctataatca cggcagaaaa gtccacattg attatttgca cggcgtcaca ctttgctatg
    13861 ccatagcatt tttatccata agattagcgg atcctacctg acgcttttta tcgcaactct
    13921 ctactgtttc tccatacccg tttttttgga tggagtgaaa cgatggcgat tgcaattggc
    13981 ctcgattttg gcagtgattc tgtgcgagct ttggcggtgg actgcgccag cggtgaagag
    14041 atcgccacca gcgtagagtg gtatccccgt tggcaaaaag ggcaattttg tgatgccccg
    14101 aataaccagt tccgtcatca tccgcgtgac tacattgagt caatggaagc ggcactgaaa
    14161 accgtgcttg cagagcttag cgtcgaacag cgcgcagctg tggtcgggat tggcgttgac
    14221 acaaccggct cgacgcccgc accgattgat gccaacggta acgtgctggc gctgcgcccg
    14281 gagtttgccg aaaacccgaa cgcgatgttc gtattgtgga aagaccacac tgcggttgaa
    14341 gaagcggaag agattacccg tttgtgccac gcgccgggca acgttgacta ctcccgctac
    14401 attggcggta tttattccag cgaatggttc tgggcaaaaa tcctgcatgt gactcgccag
    14461 gacaccgccg tggcgcaatc tgccgcatcg tggattgagc tgtgcgactg ggtgccagct
    14521 ctgctttccg gtaccacccg cccgcaggat attcgtcgcg gacgttgcag cgccgggcat
    14581 aagtcgctat ggcacgaaag ctggggtggc ttgccgccag ccagtttctt tgatgagcta
    14641 gacccgatcc tcaatcgcca tttgccttcc ccgctgttca ctgacacctg gactgccgat
    14701 attccggtgg gcaccttatg cccggaatgg gcgcagcgtc tcggcctgcc tgaaagcgtg
    14761 gtgatttccg gcggcgcgtt tgactgccat atgggcgcag ttggcgcagg cgcacagcct
    14821 aacgcactgg taaaagttat cggtacttcc acctgcgaca ttctgattgc cgacaaacag
    14881 agcgttggcg agcgggcagt gaaaggtatt tgcggtcagg ttgatggcag cgtggtacct
    14941 ggatttatcg gtctggaagc aggccaatcg gcgtttggtg atatctacgc ctggtttggt
    15001 cgcgtactcg gctggccgct ggaacagctt gccgcccagc atccggaact gaaagcgcaa
    15061 atcaacgcca gccagaaaca actgcttccg gcgctgaccg aagcatgggc caaaaatccg
    15121 tctctggatc acctgccggt ggtgctcgac tggtttaacg gccgccgcac accgaacgct
    15181 aaccaacgcc tgaaaggggt gattaccgat cttaacctcg ctaccgacgc tccgctgctg
    15241 ttcggcggtt tgattgctgc caccgccttt ggcgcacgcg caatcatgga gtgctttacc
    15301 gatcagggga tcgccgtcaa taacgtgatg gcgctgggcg gcatcgcgcg gaaaaaccag
    15361 gtcattatgc aggcctgctg cgacgtgctg aatcgcccgc tgcaaattgt tgcctctgac
    15421 cagtgctgtg cgctcggtgc ggcgattttt gctgccgtcg ccgcgaaagt gcacgcagac
    15481 atcccatcag cccagcaaaa aatggccagt gcggtagaga aaaccctgca accgcgcagc
    15541 gaacaggcac aacgctttga acagctttat cgccgctatc agcaatgggc gatgagcgcc
    15601 gaacaacact atcttccaac ttccgccccg gcacaggctg cccaggccgt tgcgactcta
    15661 taaggacacg ataatgacga tttttgataa ttatgaagtg tggtttgtca ttggcagcca
    15721 gcatctgtat ggcccggaaa ccctgcgtca ggtcacccaa catgccgagc acgttgttaa
    15781 tgcgctgaat acggaagcga aactgccctg caaactagtg ttgaaaccgc tgggcaccac
    15841 gccggatgaa atcaccgcta tttgccgcga cgcgaattac gacgatcgtt gcgctggtct
    15901 ggtggtgtgg ctgcacactt tctccccggc caaaatgtgg atcaacggcc tgaccatgct
    15961 caacaaaccg ttgctgcaat tccacaccca gtttaacgcg gcgctgccgt gggacagtat
    16021 cgatatggac tttatgaacc tgaaccagac tgcacatggc ggtcgcgagt tcggcttcat
    16081 tggcgcgcgt atgcgtcagc aacatgccgt ggttaccggt cactggcagg ataaacaagc
    16141 ccatgagcgt atcggctcct ggatgcgcca ggcggtctct aaacaggata cccgtcatct
    16201 gaaagtctgc cgttttggcg ataacatgcg tgaagtagcg gtcaccgatg gtgataaagt
    16261 tgccgcacag atcaagttcg gtttctccgt caatacctgg gcggttggcg atctggtgca
    16321 ggtggtgaac tccatcagcg acggcgatgt taacgcgctg gtcgatgagt acgaaagctg
    16381 ctacaccatg acgcctgcca cacaaatcca cggcgaaaaa cgacagaacg tgctggaagc
    16441 ggcgcgtatt gagctgggga tgaagcgttt cctggaacaa ggtggcttcc acgcgttcac
    16501 caccaccttt gaagatttgc acggtctgaa acagcttcca ggtctggccg tacagcgtct
    16561 gatgcagcag ggttacggct ttgcgggcga aggcgactgg aaaaccgccg ccctgcttcg
    16621 catcatgaag gtgatgtcaa ccggtctgca gggcggcacc tcctttatgg aggactacac
    16681 ctatcacttc gagaaaggta atgacctggt gctcggctcc catatgctgg aagtctgccc
    16741 gtcgattgcc gtagaagaga aaccgatcct cgacgttcag catctcggta ttggtggtaa
    16801 ggacgatcct gcccgactga tcttcaatac ccaaaccggt ccagcgattg tcgccagcct
    16861 gattgatctc ggcgatcgtt accgtctgct ggttaactgt atcgacacgg tgaaaacacc
    16921 gcactccctg ccgaaactgc cggtggcgaa tgcgctgtgg aaagcgcaac cggatctgcc
    16981 aactgcttcc gaagcgtgga tccttgctgg tggcgcgcac cataccgtct tcagccatgc
    17041 gctgaacctc aacgatatgc gccagttcgc cgagatgcac gacattgaaa tcacggtgat
    17101 tgataacgac acccgcctgc cagcgtttaa agacgcactg cgctggaacg aagtgtatta
    17161 cggatttcgt cgctaagtag ccacatcagg tatgtaacgc ctgatgcgac gctgacgcgt
    17221 cttatctggc ctacacgctg cgattttgta ggccggataa gcaaagcgca tccggcattg
    17281 ttttccccct cgcccctttg gggagagggc cggggtgagg ggaaacgacc tctcaggagt
    17341 caacatgtta gaagatctca aacgccaggt attagaagcc aacctggcgc tgccaaaaca
    17401 caacctggtc acgctcacat ggggcaacat cagcgccgtt gatcgcgagc gcggcgtctt
    17461 tgtgatcaaa ccttccggcg tcgattacag cgtcatgacc gctgacgata tggtcgtggt
    17521 tagcatcgaa accggtgaag tggttgaagg tacgaaaaag ccctcctccg acacgccaac
    17581 tcaccggctg ctctatcagg cattcccctc cattggcggc attgtgcata cgcactcgcg
    17641 ccacgccacc atctgggcgc agtcgggtca gtcgattcca gcaatcggca ccacccacgc
    17701 cgactatttc tacggcacca ttccctgcac ccgcaaaatg accgacgcag aaatcaacgg
    17761 cgaatatgag tgggaaaccg gtaacgtcat cgtagaaacc tttgaaaaac agggtatcga
    17821 tgcagcgcaa atgcccggcg ttctggtcca ttcccacggc ccgtttgcat ggggcaaaaa
    17881 tgccgaagat gcggtgcata acgccatcgt gctggaagag gtcgcttata tggggatatt
    17941 ctgccgtcag ttagcgccgc agttaccgga tatgcagcaa acgctgctgg ataaacacta
    18001 tctgcgtaag catggcgcga aggcatatta cgggcagtaa tgactgtata aaaccacagc
    18061 caatcaaacg aaaccaggct atactcaagc ctggtttttt gatggatttt cagcgtggcg
    18121 caggcaggtt ttatcttaac ccgacgctgg cgggacaccc cgcaagggac agaagtctcc
    18181 ttctggctgg cgacggacaa cgggccgttg caggttacgc ttgcaccgca agaatccgtg
    18241 gcgtttattc ccgccgatca ggttccccgc gctcagcata ttttgcaggg tgaacaaggc
    18301 tttcgcctga caccgctggc gttaaaggat tttcaccgcc agccggtgta tggcctttac
    18361 tgtcgcgccc atagccaatt gatgaattac gaaaagcgcc tgcgtgaagg tggcgttacc
    18421 gtctacgagg ccgatgtgcg cccgccagaa cgctatctga tggagcggtt tatcacctca
    18481 ccggtgtggg tcgagggtga tatgcacaat ggcactatcg ttaatgcccg tctgaaaccg
    18541 catcccgact atcgtccgcc gctcaagtgg gtttctatag atattgaaac cacccgccac
    18601 ggtgagctgt actgcatcgg cctggaaggc tgcgggcagc gcatcgttta tatgctgggg
    18661 ccggagaatg gcgacgcctc ctcgcttgat ttcgaactgg aatacgtcgc cagccgcccg
    18721 ctgctgctgg aaaaactcaa cgcctggttt gcaaaccacg atcccgatgt gatcatcggc
    18781 tggaacgtgg tgcagttcga tctgcgaatg ctgcaaaaac atgccgagcg ttaccgtctt
    18841 ccgctgcgtc ttgggcgcga taatagcgag ctggagtggc gcgagcacgg ctttaaaaac
    18901 ggcgtctttt ttgcccaggc taaaggtcgg ctaattatcg acggtatcga ggcgctgaaa
    18961 tccgcgttct ggaatttctc ttcattctcg ctggaaactg tcgctcagga gctattaggc
    19021 gaaggaaaat ctatcgataa cccgtgggat cgaatggacg aaattgaccg ccgtttcgcc
    19081 gaagataaac ctgcgctggc aacttataac ctgaaagatt gcgagctggt gacgcagatc
    19141 ttccacaaaa ctgaaatcat gccattttta ctcgaacgag caacggtgaa cggcctgccg
    19201 gtggatcggc acggcggttc ggtggcagcg tttggtcatc tctattttcc gcgaatgcat
    19261 cgcgctggtt atgtcgcgcc taatctcggc gaagtgccac cgcacgccag ccctggcggc
    19321 tacgtgatgg attcacggcc agggctttat gattcagtgc tggtgctgga ctataaaagc
    19381 ctgtacccgt cgatcatccg cacctttctg attgatcccg tcgggctggt tgaaggcatg
    19441 gcgcaacctg atccagagca cagtaccgaa ggttttctcg atgcctggtt ctcacgggag
    19501 aaacattgcc tgccggagat tgtgactaac atctggcacg ggcgcgatga agccaaacgt
    19561 cagggtaata agccgctttc gcaggcgctg aagatcatta tgaatgcctt ttatggcgtg
    19621 ctcggcacca ccgcctgccg cttcttcgat ccgcgcctgg catcgtcgat caccatgcgt
    19681 ggtcatcaga tcatgcggca gaccaaagca ttgattgaag cgcagggcta cgacgttatc
    19741 tacggagata ccgactcgac ttttgtctgg ctaaaaggtg cacattcgga agaagaggcg
    19801 gcgaaaatcg gtcgtgcact ggtgcagcac gttaacgcct ggtgggcgga aacgctgcaa
    19861 aaacaacggc tgaccagcgc attagaactg gagtatgaaa ctcatttctg ccgttttctg
    19921 atgccaacca ttcgcggagc cgataccggc agtaaaaagc gctacgccgg gctgattcag
    19981 gagggcgata aacagcggat ggtgtttaaa gggctggaaa ccgtgcgcac cgactggacg
    20041 ccgctggccc agcagtttca gcaggagcta tacctgcgca tcttccgcaa cgagccatat
    20101 caggaatatg tacgcgaaac catcgacaaa ctgatggcgg gtgaactgga tgcgcgactg
    20161 gtttaccgta aacgccttcg ccgtccgctg agcgagtatc agcgtaatgt gccgcctcat
    20221 gtacgcgccg ctcgccttgc cgatgaagaa aaccaaaagc gcggtcgccc cttgcaatat
    20281 cagaatcgcg gcaccattaa gtacgtatgg accaccaacg gcccggagcc gctggactac
    20341 caacgttcac cactggatta cgaacactat ctgacccgcc agctacaacc cgtggcggag
    20401 ggaatactcc cttttattga ggataatttt gctacactta tgaccgggca acttgggcta
    20461 ttttgagcaa aaaaaagagt tcgccagata ccattttgat gcgtgacgaa tgctttgcca
    20521 tccagtacca tagcgccctt tccattcctg gacctgaata acaccactac ctcataagca
    20581 cggtagcggg tggttattgc ctgcaattaa agatatagag ccgaacacat atgcctttta
    20641 cacttggtca acgctggatc agcgatacag aaagcgaatt gggacttgga accgttgtcg
    20701 cggtggatgc gcgaactgtc actttacttt tcccatctac tggtgaaaac cgtctgtacg
    20761 cacgcagtga ttcccccgtg acccgcgtga tgttcaaccc tggtgatact attaccagcc
    20821 atgacggctg gcagatgcaa gtcgaagaag taaaagaaga aaatggcttg ctgacctata
    20881 tcggtactcg cctggatact gaagagtccg gcgtagccct gcgtgaagtt ttccttgata
    20941 gcaaactggt gttcagcaaa ccgcaggacc gtctgtttgc cgggcagatt gaccgtatgg
    21001 accgctttgc gctgcgttat cgcgcgcgta agtattccag cgaacagttc cgtatgccgt
    21061 acagcggcct gcgcggtcag cgtaccagcc tgattccgca tcagctcaac atcgctcatg
    21121 atgttggtcg ccgccacgcg ccgcgcgtcc tgctggctga cgaagtgggt ttagggaaaa
    21181 ccattgaagc cgggatgatc ctgcatcagc aactgctctc tggcgctgct gaacgtgtgc
    21241 taattatcgt cccggaaacc ttacagcatc agtggctggt agaaatgctg cgccgtttca
    21301 acctgcgctt tgcgctattt gatgatgagc gttatgccga agctcagcac gatgcttaca
    21361 acccgtttga caccgaacag ctggtgattt gctcgctgga ttttgcccgt cgtagcaaac
    21421 agcgcctgga acatctctgt gaagccgaat gggacctgct ggtggtcgat gaagcgcatc
    21481 acctggtgtg gagcgaagat gcgccaagcc gtgaatatca ggccattgaa caactggcag
    21541 agcacgtgcc gggcgttctg ctgctgaccg cgaccccgga acagctgggg atggaaagcc
    21601 acttcgcccg tctgcgtctg ctggacccga accgtttcca cgattttgcg cagttcgttg
    21661 aagagcagaa aaattatcgt ccggttgcgg acgccgttgc catgctgctg gcaggtaaca
    21721 aactgagcaa tgacgaactg aacatgctcg gcgagatgat cggcgagcag gatatcgagc
    21781 cgctgttgca ggcagcaaac agcgacagcg aagatgccca gagcgcccgt caggagctgg
    21841 tttcgatgct gatggatcgc cacggcacca gccgcgtgct gttccgtaac acgcgtaacg
    21901 gtgtgaaagg attcccgaaa cgcgagctgc acaccattaa actgccgcta ccgacgcagt
    21961 atcagacggc tattaaagtc tccggcatta tgggcgcacg taaaagtgcg gaagatcgtg
    22021 ctcgcgatat gctctacccg gagcgtattt atcaggaatt tgaaggtgat aacgccacct
    22081 ggtggaactt cgatccgcgc gttgagtggc tgatgggcta cctgaccagc catcgctctc
    22141 agaaagtgct ggtgatctgc gccaaagctg ccactgcgct gcaactggag caggtactgc
    22201 gcgaacgtga aggtattcgc gctgcggtgt tccacgaagg tatgtcgatt atcgaacgtg
    22261 accgcgctgc cgcctggttt gccgaagaag acaccggcgc acaggtactg ctgtgctcag
    22321 aaatcggttc tgaaggacgt aacttccagt tcgccagcca catggtgatg tttgacctgc
    22381 cattcaaccc ggatctactg gagcagcgta ttggtcgtct ggatcgtatc ggccaggcgc
    22441 acgatattca gatccatgtg ccttatctgg agaaaaccgc tcagtcggtg ctggtgcgct
    22501 ggtatcacga aggtctggat gcatttgagc acacctgccc gaccggacgc actatttacg
    22561 atagcgtata caacgatctg attaactatc tggcttcacc ggatcaaacc gaaggctttg
    22621 acgatctgat caaaaactgc cgcgagcaac atgaagcgct gaaagcacaa ctggaacagg
    22681 gtcgtgaccg cctgctggaa atccactcca acggtggcga aaaagcccag gcactggcag
    22741 aaagcattga agagcaggat gacgatacca acctgatcgc cttcgccatg aacctgttcg
    22801 atattatcgg tatcaatcag gacgatcgcg gcgacaacat gatcgtgctg acgccgtccg
    22861 atcatatgct ggtgccggac ttccctggcc tgtcggaaga tggcatcacc atcacctttg
    22921 atcgtgaagt ggcgctggcg cgtgaagatg ctcagtttat tacctgggaa catccgctga
    22981 tccgcaacgg tctggatctg atcctttctg gcgataccgg tagcagcacg atttcactgt
    23041 taaaaaacaa agcgttgccg gtaggtacgc tgttggtgga actgatttac gtggtcgaag
    23101 cccaggcgcc gaagcagttg cagctcaacc gcttcctgcc accgacgccg gtacgtatgc
    23161 tgctggataa aaacggcaac aacctggcgg cccaggtgga gtttgaaacc tttaaccgcc
    23221 agcttaacgc ggttaaccgt cacaccggca gcaaactggt taacgccgtg cagcaggatg
    23281 ttcacgctat ccttcagctg ggtgaagcgc agatcgagaa atctgcccgt gcattgattg
    23341 atgccgcacg taacgaagcc gacgaaaaac tgtctgccga gctgtctcgt ctggaagcgc
    23401 tgcgcgcagt gaacccgaac attcgtgacg acgaactgac cgccattgag agcaaccgtc
    23461 agcaggtaat ggaaagcctg gatcaggcag gctggcgtct ggatgccctg cgtttgatcg
    23521 ttgtaacgca tcagtaacgg agccgaaaat ggggatggaa aactacaatc caccgcagga
    23581 accctggttg gttatcctgt atcaggatga ccacattatg gtggttaaca agccgagcgg
    23641 tttgttgtct gtgccgggtc gtctggaaga gcacaaagat agcgtgatga cgcgcattca
    23701 gcgtgattat ccgcaggcag aatcggtgca tcgtctggat atggctacca gcggcgtgat
    23761 tgtagtggcg ctgaccaaag ccgcggagcg ggagttaaaa cgccagttcc gcgagcgcga
    23821 gccgaaaaag cagtatgtgg cccgcgtctg ggggcatcca tcccccgcag aaggtctggt
    23881 ggatctgccg ctgatttgcg actggccaaa ccgcccgaaa cagaaagtct gttacgaaac
    23941 gggtaaacct gcgcagacgg aatatgaagt ggtggagtat gcggcggata acacggcaag
    24001 agtggtgtta aaaccgatta ccgggcgttc gcatcaattg cgtgtgcata tgctggcgct
    24061 ggggcatccg attctcggcg atcgttttta tgcatcacca gaagcgagag cgatggcacc
    24121 gcgtttgttg ctacatgcag agatgttgac gattacccat ccggcgtatg gcaatagtat
    24181 gacgtttaaa gcgccagcgg atttttaatc gtcacgctgg cgcagttcgg taggcggata
    24241 aggcgttcac gccgcatccg gcaattgtgc gccgattgcc tgatgcgacg cgggccgcgt
    24301 cttatcaggc ctacagttta aaacttattt aaaccctttc tgctgcttta tcagctcata
    24361 tgcctgctga atttcctgcg ctttctgctt cgccatctcc atcatctcag gcggcaaacc
    24421 tttcgccacc agcttatcgg gatggtgttc actcatcagc ttacggtagg cacgtttgat
    24481 ggtggtcgca tcatccgtcg gcttcacgcc cagcacatta caggcatctt ccagcgtcgg
    24541 gccacgctgc gcttgctgcc agttaccacc gccagattgc tgctgataac cgccgccaaa
    24601 ctgtgcaccg ccctgcatca tgcgcaaaaa ctggtcaaac tgggcgcggg agatccctaa
    24661 ttcttcagca atgacataca gcaccgcccg ttcattcggg tgcagtgaac catcagcaaa
    24721 cgccgcctga atctggatct ccagaaacat acgaattaag tcaaaacgac caaagcagac
    24781 actgcgaaac tggcgcatct tttcgcgcag cgggtaattg tctgattttc ccacccggaa
    24841 cgcattttgc gccgcagtac gggaagcgcc atgaagattc attcggtcca tcaactggct
    24901 ggcgatatga atatcagcct ccgtgacgcg acctttggat ttggttaaat gccccatcac
    24961 ttcaaaagtg gtggcaaaaa acagcgcctg acgctcacgc tggttggcga accacgccat
    25021 tttacggcta cgggctttat caaacatatg gccaattaac aggcctaaca ctacgcccca
    25081 aaagccgccg cccatcagta aggccacggc cacgccaatg atttttcccc aatactgcat
    25141 atattcccca aatcgacaca cggatatcag ggctatctcc cacaatataa aggtgctttt
    25201 accgttttcc ggctgcggtc aattgtggga catcgcctat aatttgcatt atcatacctg
    25261 tcattcaatg ccgtgcctaa caccactgac gctattcgga caggattaac actagcgtag
    25321 agatgacgag tacgttagtc tctgaccgtt tgtcacgcgc aacgttaccg atgatggaac
    25381 aataaaatca acgtatgaaa aaacgtatcc ccactctcct ggccaccatg attgccaccg
    25441 ccctttatag tcaacaggga ctggcagccg acctcgcctc acagtgcatg ttgggcgtgc
    25501 caagctatga ccgtcctctg gtacagggcg ataccaatga cttacccgtg actatcaatg
    25561 ctgaccacgc gaaaggggac tacccggatg acgccgtgtt tactggcagc gtggatatca
    25621 tgcagggtaa cagccgtctg caggccgacg aagtgcagct ccatcaaaaa gaggcaccag
    25681 gacaaccgga gccggtacgt accgttgatg cgctcggtaa tgtccattac gacgataacc
    25741 aggtgatcct caaagggccg aaaggctggg cgaatctgaa caccaaagat accaacgtct
    25801 gggaaggtga ttaccagatg gtgggtcgcc agggtcgcgg taaagcggac ctgatgaaac
    25861 aacgtggcga aaaccgctat accattctgg ataacggtag ctttacctcc tgtctgccgg
    25921 gttctgacac ctggagcgtg gtaggtagcg aaattattca tgaccgcgaa gaacaagttg
    25981 cggagatctg gaacgcccgc tttaaggtgg gtccggtacc gatcttttat agcccctatt
    26041 tgcagttgcc ggtgggtgac aaacgtcgct ctggtttctt gatcccgaac gccaagtaca
    26101 ccaccaccaa ctactttgag ttctacctgc catattactg gaacatcgcg ccaaatatgg
    26161 atgccaccat cacgccgcat tatatgcatc gtcgtggcaa catcatgtgg gagaacgaat
    26221 tccgctacct ctcccaggcg ggcgctggct tgatggaact ggactatctg ccttcagata
    26281 aagtctatga agatgaacac ccgaacgatg acagttcacg tcgttggtta ttctactgga
    26341 accactccgg ggtcatggat caagtgtggc gtttcaacgt cgactacacc aaggtcagcg
    26401 atcctagcta cttcaatgat ttcgataaca agtacggttc cagtactgac ggctacgcaa
    26461 cgcaaaaatt cagcgttggc tatgcggtgc aaaacttcaa tgccaccgtt tcaaccaagc
    26521 agttccaggt tttcagcgaa cagaacacca gtagctactc ggcagagccg cagttagacg
    26581 ttaattacta ccagaatgat gttggtccgt ttgatacgcg tatttacggc caggcagtgc
    26641 actttgttaa caccagagac gacatgcctg aagcaacccg tgttcacctg gaaccgacca
    26701 tcaatttgcc gctctctaat aactggggca gcatcaatac cgaagcgaag ttgctggcaa
    26761 cccattatca gcaaaccaat cttgactggt ataactccag aaacacgacc aagctggacg
    26821 aatccgttaa ccgcgtaatg ccgcaattca aagttgacgg caaaatggtc tttgaacgcg
    26881 atatggaaat gctggctccg ggttataccc aaacgctgga accgcgcgcg cagtatttgt
    26941 acgtgccgta tcgcgatcag agcgacatct ataactacga ctcgtctctg ctgcaatctg
    27001 actactctgg cctgttccgg gaccggactt acggcggtct tgaccgtatt gcctccgcta
    27061 accaggtgac gaccggtgtc acatctcgca tatatgatga tgctgccgtt gaacgtttta
    27121 atatttccgt tggtcaaatc tactatttca cggagtctcg cactggcgat gacaacataa
    27181 catgggagaa tgacgacaaa acgggttcac tggtgtgggc aggcgatact tactggcgta
    27241 tctccgagcg ttggggattg cgtggcggga ttcagtacga tacacgtctg gataacgtag
    27301 cgaccagtaa ctccagcatt gaataccgtc gggatgaaga ccgtctggta cagctgaatt
    27361 accgttacgc cagcccggaa tatattcagg ctacgctgcc taagtactat tccactgctg
    27421 agcaatataa gaatggtatt tcgcaggtag gtgctgtcgc cagctggcca attgccgatc
    27481 gttggtccat tgttggggcc tactactacg acaccaatgc gaacaagcaa gccgactcta
    27541 tgttaggtgt gcaatacagc tcctgctgct atgcaattcg cgtcggttac gagcggaagc
    27601 tgaacggttg ggataacgat aaacaacatg cggtatatga caacgcaatc ggctttaaca
    27661 tcgaacttcg cggcctgagc tccaactacg gtctgggtac gcaagagatg ctgcgttcga
    27721 acattctgcc gtatcaaaac actttgtgat ttgttgattt accacgtaat ccgcagtgcg
    27781 gttaattgaa atggaaaaag tatgaagaac tggaaaacgc tgcttctcgg tatcgccatg
    27841 atcgcgaata ccagtttcgc tgccccccag gtagtcgata aagtcgcagc cgtcgtcaat
    27901 aacggcgtcg tgctggaaag cgacgttgat ggattaatgc agtcggtaaa actgaacgct
    27961 gctcaggcaa ggcagcaact tcctgatgac gcgacgctgc gccaccaaat catggaacgt
    28021 ttgatcatgg atcaaatcat cctgcagatg gggcagaaaa tgggagtgaa aatctccgat
    28081 gagcagctgg atcaggcgat tgctaacatc gcgaaacaga acaacatgac gctggatcag
    28141 atgcgcagcc gtctggctta cgatggtctg aactacaaca cttatcgtaa ccagatccgc
    28201 aaagagatga ttatctctga agtgcgtaac aacgaggtgc gtcgtcgcat caccatcctg
    28261 ccgcaggaag tcgaatccct ggcgcagcag gtgggtaacc aaaacgacgc cagcactgag
    28321 ctgaacctga gccacatcct gatcccgctg ccggaaaacc cgacctctga tcaggtgaac
    28381 gaagcggaaa gccaggcgcg tgccattgtc gatcaggcgc gtaacggcgc tgatttcggt
    28441 aagttggcga ttgctcattc tgccgaccag caggcgctga acggcggcca gatgggctgg
    28501 ggccgtattc aggagttgcc ggggatcttc gcccaggcat taagcaccgc gaagaaaggc
    28561 gacattgttg gcccgattcg ttccggcgtt ggcttccata ttctgaaagt taacgacctg
    28621 cgcggcgaaa gcaaaaatat ctcggtgacc gaagttcatg ctcgccatat tctgctgaaa
    28681 ccgtcgccga tcatgactga cgaacaggca cgcgtgaaac tggaacagat tgctgctgat
    28741 atcaagagtg gtaaaacgac ttttgctgcc gcagcgaaag agttctctca ggatccaggc
    28801 tccgctaacc agggcggtga tctcggctgg gctacaccag atattttcga tccggccttc
    28861 cgtgacgccc tgactcgcct gaacaaaggt caaatgagtg caccggttca ctcttcattc
    28921 ggctggcatt taatcgaact gctggatacc cgtaatgtcg ataaaaccga cgctgcgcag
    28981 aaagatcgtg cataccgcat gctgatgaac cgtaagttct cggaagaagc agcaagctgg
    29041 atgcaggaac aacgtgccag cgcctacgtt aaaatcctga gcaactaatg gttaaaaccc
    29101 aacgtgttgt gatcactccc ggcgagcccg ccgggattgg cccggactta gttgtccagc
    29161 ttgcacagcg tgagtggccg gtcgaactgg ttgtttgtgc cgataccact ctccttaccg
    29221 accgggcagc gatgctcggt ttgccgctca ccctccgccc ttattccccc aactcccctg
    29281 cacaaccgca aactacgggc acattaacgc tacttcctgt cgcgctacgt gaatctgtca
    29341 ctgcggggca gttaggggtt gaaaatgggc attacgtggt ggagacgctg gcgcgagcgt
    29401 gtgatggctg tctgaacggt gaatttgctg cgctgatcac aggccccgtg cataaaggcg
    29461 tcattaacga cgcaggtatt ccgtttactg gtcataccga gtttttcgaa gagcgttcgc
    29521 aggcgaaaaa agtggtgatg atgctggcga ccgaagaact tcgcgtggcg ctggcaacga
    29581 cgcatttacc gctgcgcgat atcgcagatg ctatcacccc tgcacttttg cacgaagtga
    29641 ttgctatttt gcatcacgat ttgcggacaa aatttggtat tgccgaaccg cgcattctgg
    29701 tctgcggact gaatccgcac gcgggcgaag gcggtcatat gggtacggaa gagatagaca
    29761 ccattattcc ggtgctcgac gagctgcggg cgcaggggat gaaactcaac gggccgctgc
    29821 ctgccgatac cctgtttcag ccgaaatatc tcgataacgc cgacgccgtg ctggcgatgt
    29881 accacgatca gggtcttccc gtgctaaaat accagggctt cgggcgcggt gtgaacatta
    29941 cgctgggcct gccctttatt cgcacatcag tggaccacgg caccgcgctt gaactggcgg
    30001 gacgtggtaa agccgatgtc ggcagtttta ttacggcgct taatctcgcc atcaaaatga
    30061 ttgttaacac ccaatgaata atcgagtcca ccagggccac ttagcccgta aacgcttcgg
    30121 gcaaaacttt ctcaacgatc agttcgtgat cgacagtatt gtttctgcca ttaacccgca
    30181 aaagggccag gcgatggtcg aaatcggccc cggtctggcg gcattgaccg aaccggtcgg
    30241 cgaacgtctg gaccagctga cggtcatcga acttgaccgc gatctggcgg cacgtctgca
    30301 aacgcatcca ttcttaggcc cgaaactgac gatttatcag caggatgcga tgacctttaa
    30361 ctttggcgaa ctggccgaga aaatgggtca gccgctgcgt gttttcggca acctgcctta
    30421 taacatctcc acgccgttga tgttccatct gtttagctat actgatgcca ttgccgacat
    30481 gcactttatg ttgcaaaaag aggtggtgaa tcgtctggtt gcaggaccga acagcaaagc
    30541 gtatggtcga ttaagcgtca tggcgcaata ctattgcaac gtgatcccgg tactggaagt
    30601 tccgccgtca gcctttacac caccacccaa agtggattcc gccgtcgtgc gcctggttcc
    30661 tcatgcaacg atgcctcacc cggttaaaga tgttcgtgtg ttgagccgca tcaccaccga
    30721 agcctttaac cagcgtcgta aaaccattcg taacagcctc ggcaacctgt ttagcgtcga
    30781 ggtgttaacg ggaatgggga tcgacccggc gatgcgagcg gaaaatatct ctgtcgcgca
    30841 atactgccag atggcgaact atctggcgga gaacgcgcct ttgcaggaga gttaacgatg
    30901 atcaattcgc cccgagtgtg tattcaggtt caaagcgtct acattgaggc acaatcttca
    30961 cctgataatg aacgttacgt ttttgcttat accgtaacca tacgcaatct ggggcgagcg
    31021 ccagtgcagt tgttggggcg ttactggctg atcaccaatg gcaatggccg tgaaaccgaa
    31081 gtccagggcg aaggagtggt tggcgtccag ccacttatcg cgcctggcga agagtaccag
    31141 tacaccagcg gtgcaatcat tgaaaccccg ctgggcacca tgcagggtca ctacgaaatg
    31201 atcgatgaaa atggcgtccc tttcagcatc gacattcccg tattccgact cgccgttccc
    31261 acactcattc attaaaagaa tatggcgaca taccttattg gcgacgttca tggttgttac
    31321 gatgaactga tcgcattgct gcataaagta gaatttaccc ctgggaaaga taccctctgg
    31381 ctgacgggcg atctggtcgc gcgcgggccg ggttcgctgg atgttctgcg ctatgtgaaa
    31441 tccttaggcg acagcgtacg tctggtgctg ggcaatcacg atctgcatct gctggcggta
    31501 tttgccggga tcagccgcaa taaaccgaaa gatcgcctga caccgctgct ggaagcgccg
    31561 gatgccgacg agctgcttaa ctggctgcgt cgccagcctt tgctgcaaat cgacgaagag
    31621 aaaaagttgg tgatggccca cgcagggatc acgccgcagt gggatctgca gaccgccaaa
    31681 gagtgcgcgc gcgatgtaga agcggtgctg tcgagcgact cctatccctt cttccttgat
    31741 gccatgtacg gcgatatgcc aaataactgg tcaccggaat tgcgggggct gggaagactg
    31801 cgttttatca ccaacgcctt tacccgtatg cgtttttgct ttccaaacgg tcaactggat
    31861 atgtacagca aagaatcgcc ggaagaagcc cctgccccac tgaaaccgtg gtttgcgatt
    31921 cctggccctg tcgctgaaga atacagcatc gcctttggtc actgggcatc gctggagggc
    31981 aaaggtacgc cggaaggtat atacgcgctg gataccggct gctgctgggg tggtacatta
    32041 acctgcctgc gctgggaaga taaacagtat tttgtccagc cgtcgaaccg gcataaggat
    32101 ttgggcgagg gagaggcggt cgcgtcttaa cgaattacca ttcagggagg aataatggct
    32161 gactattttg aaatacgtgc tgattacaat cagcacacca tcacggttta tcaggcttac
    32221 aatgatgcaa tcgctgacgt cgccgtacgt gacgggcgat ttggcgcatc tttttcattc
    32281 aaccgcatga cctggattaa accctctttt atgtggatga tggagcgtag caactgggga
    32341 ctgaaaaaag atcaacaaca cattctcgca atccgaatca aacgcacctt ctttgataca
    32401 ttgctggaac aagcggtgct aaccacgccg gaagcccatg tgtatccaca tgcaggcatt
    32461 tgggaaacgt tatttgcaca agcaaatgtg tatgttcagt gggatcccga acgcagtata
    32521 aatggtaaaa aactggagca ccgatcatta cagcttggta tttcccgtaa cttgatcagt
    32581 caatttaacg aagacgccat tgttgccatt gatgatctga ctccgctggt gcgaaagtgt
    32641 cataaccttt tgataaacgg taaaacaaca caggcgaaat ccttcctgcc gccagaaaaa
    32701 atttaccctg ttagtgcagc tgcgagaaag gctttgggaa tgaaaaattc ctgagacaaa
    32761 tacgcgccta ttaagaggaa aatatctaag tgcgacaaat caacagcggg cttaatacca
    32821 gagaaattat tctggcaatg attgacgaag cagatgaacc cccatttgct gagcagcgga
    32881 cctggcatcg caggcgaatc gcttttctat gtgattgcat ccgcgttgct atcgactacg
    32941 gcttcttctg ttctgtctct cagcagtcaa taatgaacat aaccaggatg gctgaagatg
    33001 tatggataac agaaagtaaa acgacccaac tcattgatat ttggcaacaa cttaagcagc
    33061 tttatccgcc agaaactatt ccctacgtcc ataagttacc cactaaggaa ttacttgaac
    33121 tgctactttc tagtcctgat gatcttcgaa taattgaaat tcctgacatc atcggtgatg
    33181 tttttgatga ccaaacctgg attcggttat tcaaaaaaca ctttgctgat attatccaac
    33241 tacgatgtga tgtgtaacaa tccaaaatat atcgccagac gatagaccaa aaagaaagta
    33301 tttagcagat actcaacatt ctgacttctg tacagaaata gcagagttta aaaacttaaa
    33361 acttccggta ctcatcagag aaacatcctg tttcttctgc aaattcattt aatgccgaga
    33421 tggcatcctc gtttacttca ttccactgta gagtggcttg aaggtttaga cctacgcaat
    33481 gagcgcgact cacaaaatcg cgctcactgg taaaatcaaa cgtcttatcg ttcaatttgt
    33541 agtcctggca aaaaattacc gccgctccag aatctcaaag caatagctat gagagttctg
    33601 cgcatcggcg tcgtggaatt cgctgaatac cgattcccag tcatccggct cgtaatccgg
    33661 gaaatgggtg tcgccttcca cttctgcgtc gatatgcgtc agatacagtt tttgcgcttt
    33721 tggcaggaac tgctcataaa cgcgaccacc gccaatcacc atgatttctg gtacgtcacc
    33781 acacgccgcg atggcttcat ccaccgactt cacccacgtt acgcgatcgt ccgtacccgg
    33841 ttgactgctg aggataatat ttttgcgtcc tggcagcgga cgaccgatgg attcccaggt
    33901 atggcggccc ataattacgg gtttatttaa ggtgttgcgt ttaaaccagg cgagatcggc
    33961 aggcaggttc cacggcatgg cgttttccat gccgataaca cgatctaccg ctaacgccgc
    34021 aatcagacta atcattgatt tatcctggat ataaaaaatt gtcgccacta tacgtaaagc
    34081 ataaaccgtc gtcgactggc gcaaggatga tgttgagtaa aattttacgt tctgctggcg
    34141 agtccacgct ctcccactgg actcgccaca ttacaatgaa acaaaaacaa acagttagct
    34201 gtaaagtgtg atttacgtca ctctttatta ggatgagggt ttcgtttccg gttcatccgc
    34261 catgttgccg gtatgtttcc cttcttcggt tccctgccag ccatgtcgtt gaattaatga
    34321 cagatgttcg cggtcttcgg taatgatctc acttaacatc gcgctggtgc gtttatagac
    34381 cgctgcgcgg gctttggtgt cgttctcaac cattgccatc tcttccacca tctgaatatt
    34441 aaagcggcgg aacacatcgg cacgttctcg cgcttcatac ggccccagac ctaaactttc
    34501 cagtgccaga cgcccggttt tcagcgcacc ttcgaaggtt tcacgctccg gcttttcaac
    34561 gcctgcctga cgcaaacgaa tgtagtgatc gacatcgcgg gcgcgggcaa taatctgcaa
    34621 atgcgggaaa tgttctttca ccatctccgt cagttgcagg ttggtttgcg gatcgtcaat
    34681 ggcgttaatc agcacttccg ctttcgccgc tccggcagac tccagtaaat ccatccgcgt
    34741 ggcatcgcca taaaacactt tcataccaaa tttacgcaag gtttcgatat ggtccgggtc
    34801 gtgatcgagc accaccattt tcaccccgct ggagagcagt aaacgtccgg tgatctgccc
    34861 gaaacgaccg aatccggcga taatcacgcg tggctgttct tcgtcgatct catcggcttc
    34921 acgcgcttcc tcagtagaag attgctcaag gcgattgagg atcaccagca gaatcggcgt
    34981 tactgccatc gacagcgcca ccgccagggt cagcgatttc gcccactccg gctccagcac
    35041 attcgccatc tgcgccgcac caaataccac aaaggcaaac tcactaccct gccctaacaa
    35101 caccgcaaac caacgacgct gtttatttgg cacttgcaac ggtcgggcaa tcagccacag
    35161 catggcgatt ttgatgatga ggaaaccgag cagcaaaatg acaatgcgca atgggttttc
    35221 aagcagcgtg ccaaagtcta tcgacatgcc aacaccgatg aaaaacagcc ccaacagcaa
    35281 acctttaaat ggttcgatat cgctctccag cgcatgacgg tattccgagc ttgccagcag
    35341 tacgcccgcc agaaacgcgc ccatcgccat cgacaagccg acctcttcca gcagcaaacc
    35401 aaagccaaac acgaggaata acgccacggc actaaacact tcccgcaagc cagagcgggc
    35461 tacaaaacgc agcgccggac gcgtgacata gcgccccagc aataccacca gcaccagcgc
    35521 acccgccact tttaacgccg agagagcaaa tgcgcccatc gtcgtcgagg cactgctcgt
    35581 tgccagtagc ggaatcatcg ccaccagcgg gatcgccgcg atatcctgga acagcagcac
    35641 cgcaaaggca ctgcgaccca tttgcgtcac catcagattg cgttcattca tcgcctgcat
    35701 ggcaatcgcc gtagaggaga gcgccagcgt catgccgatc aattccgcga cctgccagcg
    35761 cagcccaagt aacatgcaga acagccccag caggccaccg caaatcacca tctgcaatgc
    35821 gccgccgccg aacactgccg cacgcagctt ccatagcctt tgtggatcga gttcgaggcc
    35881 gataataaac agcatcagca ccacgccaat ctcggcaaag tgcaggatag attcggcatc
    35941 ggtcaccagt cgcagccccc acgggccaat aatgcagccg gcaatcaggt agccaagtac
    36001 cgatcccaga ccaagacgta ccgcaatggg tacaatcagc gccgccgatc cgaggtagat
    36061 cagcgcctga accagcgtat ggctatccat gatgggcctc ctgccattcc agcagacgtt
    36121 gcttatagtg acgcgcctgc ccttcgaggg tttcgtcgtc acaaataaag gtgcagtgca
    36181 tggcaaacgg tggcagccag ttcagcccgc agtagattgc cgtcgcctgt agcggctgcg
    36241 acagcacatc aaagcccgga tgcgcaccaa tttcaaaatg gctttccccg ccgccggtcg
    36301 tcaccgccca cagcaaatgt ttgccatgca gcgccgtgcc gccgtgaccg taagcccagc
    36361 cgtgcgagaa aactttatcg atccaaagtt tgaggagcgg tggaatgctg taccactgca
    36421 tcgggtgctg ccagacgatc agatcggcgc gagacagcgc ctcctgctcg gcggcaatat
    36481 cgatattgaa gtcaggatag agttgataaa gagagcgaat ttcgacgcct tccagcgtcc
    36541 ttgcctgttc aagcatccgt ttattcgcat gggaatgatg cggatacgga tgcgcataaa
    36601 ttataagaat cataggtagc ccgcgtgatt acgcctttgt tttcccacaa agtgtagtca
    36661 tatattcacg cggcttatag tcaataatat tgattcagaa aagagagaaa tctgaacaat
    36721 ttaccccatt ctctgcccgc ggatcgtcat attgctggtt tgcgccagtg acatccctcg
    36781 agtctccggg gcaaacgcta cggaaatcaa caagccaaac agcgagatac ccgcccccat
    36841 tagcatcgtg ttactgatac cgtaattatt gataaagatc ggcagtgccc aggtcgaaac
    36901 aatggtgcca atacggctta aggacataat cacgcccacg gcagaggcgc ggatatctgt
    36961 cgggaagagt tcattaggat agagccactg caaattaccc ggcccgccag agaaaaaggc
    37021 atacaccgca aaggccatca ctaccagcca gatccccata tccgggatca gccccaaaac
    37081 cgccagcgcc agcgtcatca tggcaaagct gccaatcaac aatggacgcc gtccggcagt
    37141 gtttagccac agcatcggcg gaatacagcc gagcataaag aacaggctaa tcaccacgtt
    37201 ccccagtgcc gcgtttttgc caacccccaa tcccaacaaa ccaacgattt gcgggccaaa
    37261 ggtgtaaatg gcgaacattg ggatcacctg gcaggtccag atggcggcaa caaacagaac
    37321 aaaaggaaaa tggcggcgat taaacagatc gcgaaaacgg gtttgctgcg gctgctcttc
    37381 atcgaaagcc accggttcgc caaacagttt tatcatcatc tcttcgcact cttttactcg
    37441 ccctttgcgt aataaccagc ggggagattc aggcagttcg aatcgaccaa tcaaaatcaa
    37501 caaacagggg atcgccgcgc tacccagcat ccagcgccag ccgccttcca catcataaag
    37561 ccagtagccg accagatcgg cgcaggtcgc gccgacatac cacatggcgg cgataaagct
    37621 gatggaaaac gcccgctgac gggtactgga gaactcggtg atcattgagg tggcgatggg
    37681 ataatctgca ccgatgacaa tgccgataag tacccgcatc accaacagtt cgacagggga
    37741 tgagacaaac atcgtcgcca ccgatatcac gccgatggcg atgatatcaa tgaggaacat
    37801 tttgcgccgt ccgactttat cggagatata gccaaacagc gatgtgccaa cgaacagccc
    37861 ggcgagcgtt cccgcaccca gcaagccaat ccagtcagcg tccagtttca gcgccggagt
    37921 cagttgctcc agcgccacgc caatcattac cagtacataa ccatccagaa acggaccacc
    37981 acttccccac agcaaaatgc ggcggtgaat agaggagaat ttgagatcgt caaagtttct
    38041 ggacggttgc atgggttatt cctgtttttt gcaatgagag tcagtgcggc gttacgtatc
    38101 aaaccaacat cagccgtaac ggaactccac accaaaggtg ccgcgcgggt attcccactg
    38161 ttccagcgcc gaccccagcc ccagaatgcg acaggtgccg cactccagac atccggcgta
    38221 atcgaagcgc acactgccgt catcctgctt cttgtacaga cctgcggggc acgctttcac
    38281 cagcagctcc agcacctgtt tatcagcatc ggccttcaca acaatgtgcg gatgctcttc
    38341 atcgacattg aatttattga cgcccagttt gacgtccaca ttgacgggag aagtcataaa
    38401 acggtcactc ctttcatgcc atccttgatt agattgatga agcccacttt cttaccgtgg
    38461 cggaggattt tcttgcgcat cagttctggc gcactgccgt caatggtgaa caggtcacgc
    38521 gccacgccca ccgccagctc cgggtagccg ctgaacatgc gtgggttatc aaggaacgcc
    38581 ggtagtttct ggtacatgcg catatcgcgc agcgggccac tctcaagatg ctgacgatat
    38641 tccgccagtt tttgcttact gaaatcgtcg cttttcatcg ctgaaagcac ggtttttgct
    38701 gcagcttccc cggcggcaat cgccagatcc ataccgcgaa tggtaaaacc gaggttcata
    38761 cacattccgg cggcatcgcc ggcaatcaat acgccgtcac cgaccagctc cggcagcatg
    38821 ttgatgcctg cttccggcac tacgtgagcg gaatattcca ccagcttgcc acccgcgatc
    38881 agcggtgcaa cggccggatg ttgtttgaaa tcttccagca tttgcggcac cgattttttt
    38941 gcgtcatgca gatgatgcag accacaaacc agccccagcg acagggtatt ttcattggta
    39001 taaaggaagc cgccgcccat caggccatcg gtgggtgatc ccgcaaacag acaagccgcg
    39061 ccctgattac cctgcaactg aaaacggtct tcgattaccg acttcggtaa ctcgatcagt
    39121 tccttcacgc caaccgccac atccgtcggt ttaacgcgtt ttgccatccc caacttttcg
    39181 gcaaggatgg aattcacccc gtcagcaagg atcaccgttt tcgcttcaat cacatcgcca
    39241 tcggcttcta caccgacgac tttgccatcg cgctgtacga ggttatcgac acgaatcccg
    39301 gtaattaact gcgcgcccgc ttcttcggcc tgctccatca accaggcatc aaatttactg
    39361 cgcaaaacgg agtaagaacg ctgggatggc gaggtttcgt caccgttgca gtagtccata
    39421 gtcatcgctg acttttccgt cataaacgcg agtttttcat gggtgatcag gcgttctacg
    39481 ggggcggagt cggcgaaacc aggaataatg tgttccagac tgtgggcata gagacgcccg
    39541 ccggtgacgt tcttggcacc tgcggaattg ccacgctcga taactaacac ttgcgcacct
    39601 tcgcgggcga gcaccagtgc ggcaaccgaa ccggcaagcc ctgcaccgac gatgatggcg
    39661 tcaaagatat cttcggacat aagagctccc tgtcagcggc agggccaaaa tgcagccctg
    39721 ccagagtgga tcaacgcgct aaagctgcgg tcagcgccgg aaggatcttc acggcgtcgc
    39781 caacaatgcc gtaatccgcg tactggaaga tcggcgcatt tttatctttg ttgatggcga
    39841 aaatggtttg cgatgcatta gcaccaacca tgtgctggat ctgcccggag atccccaccg
    39901 ccaggtacag ttcaggtttc agcatcaggt tggagatacc gacatagcgt tcgtgctcca
    39961 tccatttttc gttttccgcc accggacgag aacaggccaa ctccgcacct atcgccttgc
    40021 aaagctgttc tgccagcgca atgttctctt tgctgccaat accgcgaccg acgctgacca
    40081 ccagacgggc tttgtcgaga tcgacgctgt tgctctggcg cgcctgggtt gccgtgcggg
    40141 taatcgccac agccggagcc tgccactcca cggtgtgcgt ttcgccagtg cgtgacgcgt
    40201 ctggctgagc cgcatcgaac gtgccgctgc tgatggtcag taccgcatac ggcgtggcaa
    40261 tgcgttcttc gccaatcgcc agaccaccgt aaaccatgtg tttcactgtc gctttaccgt
    40321 cctgtacgct gacggtgctg gcatcgttag acaccgccgc tttaaggcga taacccagtt
    40381 ttgccgccag taatttgccg cgacgggtgt ttggcagcag caccaggccg tctgcgccgt
    40441 gctggcgaat agtgtcagcc atgacaccgg cgtaatcttc gatcatccga tcgtccggtt
    40501 tgccgtttaa tttccagaca tgattagcgc cgagctggat tgcctgtacg ccgtcggcat
    40561 cattgaggac aaaggtgttg atttgattag ctaaagcctg cgcaccgttc atcagttccg
    40621 gcagacgaga aggggtatcg ctgaataccc agacttgaga aaacgtgttc atagcatccc
    40681 ctgtaattaa atgactttgc gaagattttc agcaaatgcg gcgatctgtt cttcgccgtc
    40741 gccttcaatc acgatgcgct gacgttcgcg ctgtttcggc gcagcaacct gttgttcaga
    40801 ccaggcgtcc acggcgttaa aaccgatatc cgccgccgac catacctgga ctggcttttt
    40861 cgccgcgccg agaatggctt tcatcgaagg aatttgtggg gagttgatat cagtggaaac
    40921 agcaacaacc gcaggcagcg gaatgcttaa ggtttcggtt tcatcttcca gttcgcgctc
    40981 aacggtgagg gtatctgccg tcagggagat aattttgctg acgccgttaa ctgccggaat
    41041 attgaggatt tcgcccacca gcagaccaac ctgctgggca taaaggtcgg aagaaccatc
    41101 gccacagagg atcaaatcaa agcctgcttt ctgggcggct gcagccagtg cgctcgccgt
    41161 ttgttgcggc agtgcctgct cgaactggtc atcaatcacc acaatcagtt catccgggcc
    41221 gcgcgatagc acatctttac gccctttggc gttggtcagg gctttaccgc ccacacttaa
    41281 ggccgtcacc tgcgcctctg ctgcctgttg ctttagctgg caagccgctt caatagcgtt
    41341 cagatcgtat tggcttattt tggcatcggc tttgctgaag tctaatgaac catcagcatt
    41401 attgaccgca atatcctgtt catcaggcac gcacttatag caagtaataa tcttcattgc
    41461 atctccagaa atcatgaagg aaacgtcgtt catatcggtg gtgttaatta cagatatcac
    41521 gccctttaat ttggaaataa aactttaatc accaatattg aaaatgtcac acgcagataa
    41581 atccaacttt caatattgtt aagttcctca ccaatattga aaacatggcg tagaaaaaag
    41641 aaattttcaa tattgcttta tggatcacca atattgaaag tcagatgcta tttttttact
    41701 cacaacacaa cataacaaac tgattaataa tcaaaattaa cgagaaaatc gccctctatt
    41761 cacccgaaac aaaaatgtga taacaatcac agaatgcagc ttattgaata cccattatga
    41821 gt
//
LOCUS       DAZFQD010000027        41672 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.027, whole genome shotgun sequence.
ACCESSION   DAZFQD010000027 DAZFQD010000000
VERSION     DAZFQD010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 41672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41672
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.027"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(244..1836)
                     /gene="lsrK"
                     /locus_tag="ACHKTW_001522"
     CDS             complement(244..1836)
                     /gene="lsrK"
                     /locus_tag="ACHKTW_001522"
                     /EC_number="2.7.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416028.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071518 - autoinducer-2 kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer-2 kinase"
                     /protein_id="HFO6462574.1"
                     /translation="MARLFTPSESKYYLMALDAGTGSIRAVIFDLEGNQIAVGQAEWR
                     HLAVPDVPGSMEFDLNKNWQLACECMRQALHNAGIAPEYIAAVSACSMREGIVLYNNE
                     GAPIWACANVDARAAREVSELKELHNNTFENEVYRATGQTLALSAIPRLLWLAHHRSD
                     IYRQASTITMISDWLAYMLSGELAVDPSNAGTTGLLDLTTRDWKPALLDMAGLRADIL
                     SPVKETGTLLGVVSSQAAELCGLKAGTPVVVGGGDVQLGCLGLGVVRPAQTAVLGGTF
                     WQQVVNLAAPVTDPEMNVRVNPHVIPGMVQAESISFFTGLTMRWFRDAFCAEEKLIAE
                     RLGIDTYTLLEEMASRVPPGSWGVMPIFSDRMRFKTWYHAAPSFINLSIDPDKCNKAT
                     LFRALEENAAIVSACNLQQIADFSNIHPSSLVFAGGGSKGKLWSQILADVSGLPVNIP
                     VVKEATALGCAIAAGVGAGIFSSMAETGERLVRWERTHTPDPEKHELYQDSRDKWQAV
                     YQDQLGLVDHGLTTSLWKAPGL"
     gene            complement(1915..2868)
                     /gene="lsrR"
                     /locus_tag="ACHKTW_001523"
     CDS             complement(1915..2868)
                     /gene="lsrR"
                     /locus_tag="ACHKTW_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LsrR"
                     /protein_id="HFO6462575.1"
                     /translation="MTINDSAISEQGMCEEEQVARIAWFYYHDGLTQSEISDRLGLTR
                     LKVSRLLEKGHQSGIIRVQINSRFEGCLEYETQLRRQFSLQHVRVIPGLADADVGGRL
                     GIGAAHMLMSLLQPQQMLAIGFGEATMNTLQRLSGFISSQQIRLVTLSGGVGSYMTGI
                     GQLNAACSVNIIPAPLRASSADIARTLKNENCVKDVLLAAQAADVAIVGIGAVSQQDD
                     ATIIRSGYISQGEQLMIGRKGAVGDILGYFFDAKGDVVTDIKIHNELIGLPLSSLKTI
                     PVRVGVAGGENKAEAIAAAMKGGYINALVTDQDTAAAILRS"
     gene            3117..4652
                     /gene="lsrA"
                     /locus_tag="ACHKTW_001524"
     CDS             3117..4652
                     /gene="lsrA"
                     /locus_tag="ACHKTW_001524"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter ATP-binding
                     protein LsrA"
                     /protein_id="HFO6462576.1"
                     /translation="MQTSDTRALPLLCARSVYKQYSGVNVLKGIDFTLHQGEVHALLG
                     GNGAGKSTLMKIIAGITPADSGTLEIGGNNYARLTPVHAHQLGIYLVPQEPLLFPSLS
                     IKENILFGMAKKQLSMQKMKNLLAALGCQFDLHSLAGSLDVADRQMVEILRGLMRDSR
                     ILILDEPTASLTPAETERLFSRLQELLATGVGIVFISHKLPEIRQIADRISVMRDGTI
                     ALSGKTSELSTDDIIQAITPAVREKSLSASQKLWLELPGNRPQHAAGTPVLTLENLTG
                     EGFRNVSLTLNAGEILGLAGLVGAGRTELAETLYGLRTLRGGRIMLNGKEINKLSTGE
                     RLLRGLVYLPEDRQSSGLNLDASLAWNVCALTHNLRGFWAKTAKDNATLERYRRALNI
                     KFNQPEQAARTLSGGNQQKILIAKCLEASPQVLIVDEPTRGVDVSARNDIYQLLRSIA
                     AQNVAVLLISSDLEEIELMADRVYVMHQGEIAHSALTGRDINVETIMRVAFGDSQRQE
                     ASC"
     gene            4646..5674
                     /gene="lsrC"
                     /locus_tag="ACHKTW_001525"
     CDS             4646..5674
                     /gene="lsrC"
                     /locus_tag="ACHKTW_001525"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrC"
                     /protein_id="HFO6462577.1"
                     /translation="MLKFIQNNREITALLAVVLLFVLPGFLDRQYLSVQTLTMVYSSA
                     QILILLAMGATLVMLTRNIDVSVGSITGMCAVLLGMLLNAGYSLPVACVATLLLGLLA
                     GFFNGVLVAWLKIPAIVATLGTLGLYRGIMLLWTGGKWIEGLPAELKQLSAPLLFGVS
                     AIGWLTIILVAFMAWLLAKTAFGRSFYATGDNLQGARQLGVRTEAIRIVAFSLNGCMA
                     ALAGIVFASQIGFIPNQTGTGLEMKAIAACVLGGISLLGGSGAIIGAVLGAWFLTQID
                     SVLVLLRIPAWWNDFIAGLVLLAVLVFDGRLRCALERNLRRQKYARFMTPPPSVKPAS
                     SGKNREAA"
     gene            5674..6666
                     /gene="lsrD"
                     /locus_tag="ACHKTW_001526"
     CDS             5674..6666
                     /gene="lsrD"
                     /locus_tag="ACHKTW_001526"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrD"
                     /protein_id="HFO6462578.1"
                     /translation="MRIRYGWELALAALLVIEIVAFGAINPRMLDLNMLLFSTSDFIC
                     IGIVALPLTMVIVSGGIDISFGSTIGLCAIALGVLFQSGVPMPLAILLTLLLGALCGL
                     INAGLIIYTKVNPLVITLGTLYLFAGSALLLSGMAGATGYEGIGGFPMAFTDFANLDV
                     LGLPVPLIIFLICLLVFWLWLHKTHAGRNVFLIGQSPRVALYSAIPVNRTLCALYAMT
                     GLASAVAAVLLVSYFGSARSDLGASFLMPAITAVVLGGANIYGGSGSIIGTAIAVLLV
                     GYLQQGLQMAGVPNQVSSALSGALLIVVVVGRSVSLHRQQIKEWLARRANNPLP"
     gene            6678..7700
                     /gene="lsrB"
                     /locus_tag="ACHKTW_001527"
     CDS             6678..7700
                     /gene="lsrB"
                     /locus_tag="ACHKTW_001527"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein LsrB"
                     /protein_id="HFO6462579.1"
                     /translation="MTLHRFKKIALLSALGIAAISMNVQAAERIAFIPKLVGVGFFTS
                     GGNGAQQAGKELSVDVTYDGPTEPSVSGQVQLINNFVNQGYNAIIVSAVTPDGLCPAL
                     KRAMQRGVRVLTWDSDTKPECRSYYINQGTPAQLGGMLVDMAARQVNKDKAKVAFFYS
                     SPTVTDQNQWVKEAKAKIAKEHPGWEIVTTQFGYNDATKSLQTAEGILKAYSDLDAII
                     APDANALPAAAQAAENLKNDKVAIVGFSTPNVMRPYVERGTVKEFGLWDVVQQGKISV
                     YVADALLKKGSMKTGDKLDIQGVGQVEVSPNSVQGYDYEADGNGIVLLPERVIFNKEN
                     IGKYDF"
     gene            7727..8602
                     /gene="lsrF"
                     /locus_tag="ACHKTW_001528"
     CDS             7727..8602
                     /gene="lsrF"
                     /locus_tag="ACHKTW_001528"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HFO6462580.1"
                     /translation="MADLDDIKDGKDFRTDQPQQNIPFILKGCGALDWGMQSRLSRIF
                     NPKTGKTVMLAFDHGYFQGPTTGLERIDINIAPLFEHADVLMCTRGILRSVVPPATNK
                     PVVLRASGANSILAELSNEAVALSMDDAVRLNSCAVAAQVYIGSEYEHQSIKNIIQLV
                     DAGMKVGMPTMAVTGVGKDMVRDQRYFSLATRIAAEMGAQIIKTYYVEKGFERIVAGC
                     PVPIVIAGGKKLPEREALEMCWQAIDQGASGVDMGRNIFQSDHPVAMMKAVQAVVHHN
                     ETADRAYELYLSEKQ"
     gene            8626..8916
                     /gene="lsrG"
                     /locus_tag="ACHKTW_001529"
     CDS             8626..8916
                     /gene="lsrG"
                     /locus_tag="ACHKTW_001529"
                     /EC_number="5.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione
                     isomerase"
                     /protein_id="HFO6462581.1"
                     /translation="MHVTLVEINVHEDKIDEFIEVFRQNHLGSVQEEGNLRFDVLQDP
                     EVNSRFYIYEAYKDEDAVAFHKTTPHYKTCVAKLESLMTGPRKKRLFNGLMP"
     gene            8973..9731
                     /gene="tam"
                     /locus_tag="ACHKTW_001530"
     CDS             8973..9731
                     /gene="tam"
                     /locus_tag="ACHKTW_001530"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030798 - trans-aconitate
                     2-methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HFO6462582.1"
                     /translation="MSDWNPSLYLHFAAERSRPAVELLARVPLENIEYVADLGCGPGN
                     STALLHQRWPAARITGIDSSPAMIAEARSALPDCQFVEADIRNWQPEQALDLIFANAS
                     LQWLPDHYELFPHLVSLLNPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLA
                     GVHAYYDILSEAGCEVDIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFL
                     KRYHQMLEEQYPLQENGQILLAFPRLFIVARRME"
     gene            complement(9735..10649)
                     /gene="yneE"
                     /locus_tag="ACHKTW_001531"
     CDS             complement(9735..10649)
                     /gene="yneE"
                     /locus_tag="ACHKTW_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family protein"
                     /protein_id="HFO6462583.1"
                     /translation="MIVRPQQHWLRRIFVWHGSVLSKISSRLLLNFLFSIAVIFMLPW
                     YTHLGIKFTLAPFSILGVAIAIFLGFRNNAGYARYVEARKLWGQLMIASRSLLREVKT
                     TLPDSASVREFARLQIAFAHCLRMTLRKQPQAEVLAHYLKTEDLQPVLASNSPANRIL
                     LIMGEWLAVQRRNGQLSDILFISLNDRLNDISAVLAGCERIAYTPIPFAYTLILHRTV
                     YLFCIMLPFALVVDLHYMTPFISVLISYTFISLDCLAEELEDPFGTENNDLPLDAICN
                     AIEIDLLQMNDEAEIPAKILPDRHYQLT"
     gene            complement(10856..12307)
                     /gene="uxaB"
                     /locus_tag="ACHKTW_001532"
     CDS             complement(10856..12307)
                     /gene="uxaB"
                     /locus_tag="ACHKTW_001532"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HFO6462584.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLAGQKAKGTLPARLTFA
                     LAALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDRVIGTQELVAIVLAEKDHWE
                     QDLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
     gene            complement(12534..13952)
                     /locus_tag="ACHKTW_001533"
     CDS             complement(12534..13952)
                     /locus_tag="ACHKTW_001533"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFO6462585.1"
                     /translation="MHVHPISTFRLFQEGHLLRNSIAIFALTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGVFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLAINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIALIVSVIASVVIGGAGSLAF
                     PLPALIWCAVRYTPQVTCLLTFVTGAVEIVLVANSVIDISVGSPFSIPQMFSARLGIA
                     TMAICPIMVSFSVAAINSLMKQVALRADFDFLTQVYSRSGLYEALKSPSLKQTQHLTV
                     MLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGLVARMGGEEFAVAVPSVNP
                     VDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRASYRTLTDDFNKLMVEADTC
                     LYRSKKDGRNRTSTMRYGEEVV"
     gene            complement(14091..14450)
                     /gene="yneG"
                     /locus_tag="ACHKTW_001534"
     CDS             complement(14091..14450)
                     /gene="yneG"
                     /locus_tag="ACHKTW_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HFO6462586.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGVSLSEEQQR
                     YIVAVIYHWLVVQMNQP"
     gene            complement(14450..15376)
                     /gene="glsB"
                     /locus_tag="ACHKTW_001535"
     CDS             complement(14450..15376)
                     /gene="glsB"
                     /locus_tag="ACHKTW_001535"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HFO6462587.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINAGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            complement(15440..16828)
                     /gene="sad"
                     /locus_tag="ACHKTW_001536"
     CDS             complement(15440..16828)
                     /gene="sad"
                     /locus_tag="ACHKTW_001536"
                     /EC_number="1.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004030 - aldehyde dehydrogenase [NAD(P)+]
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate-semialdehyde dehydrogenase"
                     /protein_id="HFO6462588.1"
                     /translation="MTITPATHAISINPATGEQLSVLPWAGANDIENALQLAAAGFRD
                     WRETNIDYRAEKLRGIGKALRARSEEMAQMITREMGKPINQARAEVAKSANLCDWYAE
                     HGPAMLKAEPTLVENQQAVIEYRPLGTILAIMPWNFPLWQVMRGAVPIILAGNGYLLK
                     HAPNVMGCAQLIAQVFKDAGIPQGVYGWLNADNDGVSQMIKDSRIAAVTVTGSVRAGA
                     AIGAQAGAALKKCVLELGGSDPFIVLNDADLELAVKAAVAGRYQNTGQVCAAAKRFII
                     EEGIASAFTERFVAAAAALKMGDPRDEENALGPMARFDLRDELHHQVEKTLAQGARLL
                     LGGEKMAGAGNYYPPTVLANVTPEMTAFREEMFGPVAAITVAKDAEHALELANDSEFG
                     LSATIFTTDETQARQMAARLECGGVFINGYCASDARVAFGGVKKSGFGRELSHFGLHE
                     FCNIQTVWKDRI"
     gene            16929..17810
                     /gene="yneJ"
                     /locus_tag="ACHKTW_001537"
     CDS             16929..17810
                     /gene="yneJ"
                     /locus_tag="ACHKTW_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFO6462589.1"
                     /translation="MDLTQLEMFNAVAEAGSITQAAAKVHRVPSNLTTRLRQLETELG
                     VDLFIRENQRLRLSPAGHNFLRYSQQILALVDEARSVVAGDEPQGLFSLGSLESTAAV
                     RIPATLAEFNHRYPKIQFSLSTGPSGTMLEGVLEGKLNAAFIDGPINHTAIDGIPVYR
                     EELMIVTPQGHAPVIRASQVNGSNIYAFRANCSYRRHFESWFHADGAAPGTIHEMESY
                     HGMLACVVAGAGIALIPRSMLESMPGHHQVEAWPLAEQWRWLTTWLVWRRGAKTRPLE
                     AFIQLLDVPDSAKQGYQ"
     gene            17888..19003
                     /gene="yneK"
                     /locus_tag="ACHKTW_001538"
     CDS             17888..19003
                     /gene="yneK"
                     /locus_tag="ACHKTW_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HFO6462590.1"
                     /translation="MVTPVSISNYISLPDDFPARNIAPQVKEVLKDFIDALSTIICDE
                     EWRTSLNINSATKKIFNNLDNLSYIQRTSFRGNDTLYNEKIQFKLTYPVKNGRHKENI
                     EFQVLINLSPIYLDNFRHDGEINIFCAPNPKPVTMGRVFQTGVERVPFLFLNDFIEQF
                     PMINPGAPIKRAHTPHIEPLPPDHHTAADYLRQFDLLVLNFISRGNFVILPRLWNNSE
                     VHRWFVNKDPNLNTAILDITDSELKEDLLQSLMDSLGSNKHVLPEVCICFLSLLAEQE
                     SPHFQDLFLFFANMLLHYHQFMNPNESDLNDVLMPASLSDDKIIKHMARRTLKLFVKN
                     ETPPKVTHEDLVKNRPRSPVRPPIPATSKTPDLPERH"
     gene            19153..20343
                     /gene="ydeA"
                     /locus_tag="ACHKTW_001539"
     CDS             19153..20343
                     /gene="ydeA"
                     /locus_tag="ACHKTW_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HFO6462591.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALITLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(20368..21033)
                     /gene="marC"
                     /locus_tag="ACHKTW_001540"
     CDS             complement(20368..21033)
                     /gene="marC"
                     /locus_tag="ACHKTW_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HFO6462592.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            21245..21679
                     /gene="marR"
                     /locus_tag="ACHKTW_001541"
     CDS             21245..21679
                     /gene="marR"
                     /locus_tag="ACHKTW_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HFO6462593.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            21699..22082
                     /gene="marA"
                     /locus_tag="ACHKTW_001542"
     CDS             21699..22082
                     /gene="marA"
                     /locus_tag="ACHKTW_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HFO6462594.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            22114..22332
                     /gene="marB"
                     /locus_tag="ACHKTW_001543"
     CDS             22114..22332
                     /gene="marB"
                     /locus_tag="ACHKTW_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HFO6462595.1"
                     /translation="MKPLSSAIAAALILFSAQGVAEQTTQPVVTSCANVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(22389..23828)
                     /locus_tag="ACHKTW_001544"
     CDS             complement(22389..23828)
                     /locus_tag="ACHKTW_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFO6462596.1"
                     /translation="MSGFKKGFLWGGAVAAHQLEGGWNEGGKGISIADVMTAGAHGVP
                     REVTEGVIDGLNYPNHEAIDFYHRYKTDIQLFAEMGFKCFRTSIAWTRIFPQGDEQEP
                     NEEGLQFYDDLFDECLKQGMEPVVTLSHFEMPYHLVTKYGGWRNRKLIDFFIRFASTV
                     FTRYKEKVKYWMTFNEINNQVNFSESLCPFTNSGILYSPEEDINEREQIMYQAVHYEL
                     VASALAVQTGKSINPEFNIGCMIAMCPIYPLTCAPNDMMMATKAMHRRYWFTDVHARG
                     YYPQHMLNYFARKGFNLDITPEDNAILASGCVDFIGFSYYMSFTTQFSPDNPQLDYVE
                     PRDLVSNPYIDTSEWGWQIDPAGLRYSLNWFWDHFQLPLFIVENGFGAVDQRQADGTV
                     NDHYRIDYFASHIREMKKAVVEDGVDLIGYTPWGCIDLVSAGTGEMKKRYGMIYVDKD
                     NEGKGTLERIRKASFYWYRDLIANNGENI"
     gene            complement(23853..25526)
                     /locus_tag="ACHKTW_001545"
     CDS             complement(23853..25526)
                     /locus_tag="ACHKTW_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HFO6462597.1"
                     /translation="MNIKTLNVSLLSFSIITALFPLNAMATKLTIEQRLELLENELSQ
                     NKQELKATQNELGVYKSRLSTLQKSITENKYKSASLAEISATSPVADNIKNENGEQNS
                     SAAAHTINGSQQVAVIESKGDKTTIESVTLKDISKYIKDDIGFSYQGYFRSGWGTGNH
                     GSPQTYAAGSLGRFGNEMSGWFDLTLNQRVYNQDGKTANAVVTYDGNVGEQYNDAWFG
                     DSANENIMQFSDIYLTTRGFLPFAPEADFWVGKHKLPQYEIQMLDWKTLTTDVAAGVG
                     IENWALGVGLFDMSLSRDDVDVYSRDFSRTSQMNTNSVDVRYRNIPLWDDATLSLMAK
                     YSAPNKTDQQQDNENDNSYFEMKDSWMLTSVLRQNLQRDTFNEFTLQVANNSYASSFA
                     SFSDASNTMAHGRYYYGDHTNGIAWRLISQGEMYLTDNIIMANALVYSHGEDVYSYES
                     GAHSDFDSIRTVIRPAWIWNTWNQTGLELGWFKQQNKTQQGVTLNESAYKTTLWHALK
                     VGESILGSRPEIRFYGTYINILDNELSNFKFNENSKDEFMAGIQAEVWW"
     gene            complement(25582..25893)
                     /locus_tag="ACHKTW_001546"
     CDS             complement(25582..25893)
                     /locus_tag="ACHKTW_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFO6462598.1"
                     /translation="MFADEELVMELLVNAGQARSDAMEAIRCAGQKDWQGATQLMASS
                     ESACLQAHKIQTALISQDEGCGKIKVNLILIHAQDHLMNAILCQDLAREIISLRKELH
                     A"
     gene            complement(25921..27243)
                     /locus_tag="ACHKTW_001547"
     CDS             complement(25921..27243)
                     /locus_tag="ACHKTW_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001736301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFO6462599.1"
                     /translation="MGLMASFERGMERFLVPVAVKLNSQKHVAAVRDGFVFTFPIIMA
                     SSLIILINFAILSPDGFIAGLLHLNSIFPNLEKAQAIFTPVMNGSVNIMSIMIAFLVA
                     RNMAISYEQDDLLCGLTAIGAFFIVYTPYQMIDGQAFLTTKYLGAQGLFVAVIVALIT
                     SEIFCRLARNPKITITMPAAVPPAVARSFKVLLPIFFVMVFFSALNYCLTLISPAGLN
                     DLIYTLIQTPLKHMGTNIFAVIILGAVGNFLWVLGIHGPNTTSAIRETVFSEANLENL
                     SWAAQHGTTWGAPYPITWTSINDAFANCGGSGMTLGLLLAIFIASKRAEYRDLAKMSF
                     IPGIFNINEPIMFGLPIVLNPIMMVPFIMVPIVNCAIGYFFVSMEIIPPVAYAVPWTT
                     PGPLIAFLGTGGNWLALLVGFLCLGVATMIYLPFVIAANKVNNMTTNG"
     gene            complement(27358..27669)
                     /locus_tag="ACHKTW_001548"
     CDS             complement(27358..27669)
                     /locus_tag="ACHKTW_001548"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000722572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFO6462600.1"
                     /translation="MKKILLVCAAGMSTSMLVKRMIDHANAISLEVNISALAIAEAKG
                     KIKNNEVDVVLLGPQVRFQKPEIEAVAQGKMPVAVIEMKDYGTMNGQAVLEFAMKLLQ
                     E"
     gene            27868..28566
                     /locus_tag="ACHKTW_001549"
     CDS             27868..28566
                     /locus_tag="ACHKTW_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000577178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFO6462601.1"
                     /translation="MIFQKIARLLKSEINGNSWHVGDLLPSEAELAVRYNVSRNTLRK
                     ALSLLEGEGIIHRKHGSGTYIQKKNFVAHIDHMNSFSEIAHKSGKEAGSQIMKFEVQD
                     ASPTIATELNLVTGEQVYYIKRLRFIEDNAAQLEETWMSVARFPDLTVSHMQKSKFSY
                     IENECGIKIIGTFETFSPTFPTPEIASILRISPRDPILKIQTQAVDSNSIPLDYSLLY
                     SNIFEFQVKYFFPR"
     gene            complement(28611..29510)
                     /gene="eamA"
                     /locus_tag="ACHKTW_001550"
     CDS             complement(28611..29510)
                     /gene="eamA"
                     /locus_tag="ACHKTW_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HFO6462602.1"
                     /translation="MSRKDGVLALLVVVVWGLNFVVIKVGLHNMPPLMLAGLRFMLVA
                     FPAIFFVARPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIV
                     LGAFTFGERLHGKQLAGIALAIFGVLVLIEDSLNGQHVAMLGFMLTLAAAFSWACGNI
                     FNKKIMSHSTRPAVMSLVIWSALIPIIPFFVASLILDGSATMIHSLVTIDMTTILSLM
                     YLAFVATIVGYGIWGTLLGRYETWRVAPLSLLVPVVGLASAALLLDERLTGLQFFGAV
                     LIMTGLYINVFGLRWRKAVKVRG"
     gene            29704..30891
                     /gene="ydeE"
                     /locus_tag="ACHKTW_001551"
     CDS             29704..30891
                     /gene="ydeE"
                     /locus_tag="ACHKTW_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
                     /protein_id="HFO6462603.1"
                     /translation="MNLSLRRSTSALLASSLLLTIGRGATLPFMTIYLSRQYSLSVDL
                     IGYAMTIALTIGVVFSLGFGILADKFDKKRYMLLAITAFASGFIAIPLVNNVTLVVLF
                     FALINCAYSVFATVLKAWFADNLSSTSKTKIFSINYTMLNIGWTIGPPLGTLLVMQSI
                     NLPFWLAAICSAFPMLFIQIWVKRSEKIIATETGSVWSPKVLLQDKALLWFTCSGFLA
                     SFVSGAFASCISQYVMVIADGDFAEKVVAVVLPVNAAMVVTLQYSVGRRLNPVNIRAL
                     MIAGTLCFVIGLVGFIFSGNSLLLWGMSAAVFTVGEIIYAPGEYMLIDHIAPPGMKAS
                     YFSAQSLGWLGAAINPLVSGVVLTSLPPSSLFIILALVIIAAWVLMLKGIRAKPWGQP
                     ALC"
     gene            31018..31113
                     /gene="mgtS"
                     /locus_tag="ACHKTW_001552"
     CDS             31018..31113
                     /gene="mgtS"
                     /locus_tag="ACHKTW_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtS"
                     /protein_id="HFO6462604.1"
                     /translation="MLGNMNVFMAVLGIILFSGFLAAYFSHKWDD"
     gene            31113..31217
                     /gene="mgtT"
                     /locus_tag="ACHKTW_001553"
     CDS             31113..31217
                     /gene="mgtT"
                     /locus_tag="ACHKTW_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtT"
                     /protein_id="HFO6462605.1"
                     /translation="MNGDNPSPNRPLVTVVYKGPDFYDGEKKPPVNRR"
     gene            complement(31332..32222)
                     /gene="dgcZ"
                     /locus_tag="ACHKTW_001554"
     CDS             complement(31332..32222)
                     /gene="dgcZ"
                     /locus_tag="ACHKTW_001554"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcZ"
                     /protein_id="HFO6462606.1"
                     /translation="MIKKTTEIDAILLNLNKAIDAHYQWLVSMFHSVVARDASKPEIT
                     DNHSYGLCQFGRWIDHLGPLDNDELPYVRLMDSAHQHMHNCGRELMLAIVENHWQDAH
                     FDAFQEGLLSFTAALTDYKIYLLTIRSNMDVLTGLPGRRVLDESFDHQLRNAEPLNLY
                     LMLLDIDRFKLVNDTYGHLIGDVVLRTLATYLASWTRDYETVYRYGGEEFIIIVKAAN
                     DEEACRAGVRICQLVDNHAITLSEGHINITVTAGVSRAFPEEPLDVVIGRADRAMYEG
                     KQTGRNRCMFIDEQNVINRV"
     gene            complement(32477..32869)
                     /gene="ydeI"
                     /locus_tag="ACHKTW_001555"
     CDS             complement(32477..32869)
                     /gene="ydeI"
                     /locus_tag="ACHKTW_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family stress tolerance OB fold protein"
                     /protein_id="HFO6462607.1"
                     /translation="MKFQAIVLASFLVMPYALADDQGGLKQDAAPPPPHAIEDGYRGT
                     DDAKKMTVDFANTMHDGASVSLRGNLISHKGEDRYVFRDKSGEINVVIPAAVFDGREV
                     QPDQMINISGSLDKKSAPAVVRVTHLQK"
     gene            33145..33663
                     /gene="ydeJ"
                     /locus_tag="ACHKTW_001556"
     CDS             33145..33663
                     /gene="ydeJ"
                     /locus_tag="ACHKTW_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HFO6462608.1"
                     /translation="MNINSDKIVQLADTDTIENLTSALSQRLIADQLRLTTAESCTGG
                     KLASALCAAEDTPKFYGAGFVTFTDQAKMKILSVSQQSLERYSAVSEKVAAEMATGAI
                     ERADADVSIAITGYGGPEGGEDGTPAGTVWFAWHIKGQTYTAVMHFAGDCETVLALAV
                     RFALAQLLQLLL"
     gene            complement(33708..35753)
                     /gene="dcp"
                     /locus_tag="ACHKTW_001557"
     CDS             complement(33708..35753)
                     /gene="dcp"
                     /locus_tag="ACHKTW_001557"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HFO6462609.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELLTRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDGNDTRAIIQRLVEIRAQQAKLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGLRFREAILSRGNSEDLERLYRQWRGKAPQIMPMLQHRGLNI"
     gene            35890..36636
                     /gene="ydfG"
                     /locus_tag="ACHKTW_001558"
     CDS             35890..36636
                     /gene="ydfG"
                     /locus_tag="ACHKTW_001558"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HFO6462610.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELKDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWCNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            36725..37411
                     /gene="rspR"
                     /locus_tag="ACHKTW_001559"
                     /gene_synonym="ydfH"
     CDS             36725..37411
                     /gene="rspR"
                     /locus_tag="ACHKTW_001559"
                     /gene_synonym="ydfH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator rspR"
                     /protein_id="HFO6462611.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            37589..37792
                     /gene="ydfZ"
                     /locus_tag="ACHKTW_001560"
     CDS             37589..37792
                     /gene="ydfZ"
                     /locus_tag="ACHKTW_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HFO6462612.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(37828..39288)
                     /gene="ydfI"
                     /locus_tag="ACHKTW_001561"
     CDS             complement(37828..39288)
                     /gene="ydfI"
                     /locus_tag="ACHKTW_001561"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFO6462613.1"
                     /translation="MGNNLLSAKATLPVYDRNNLAPRIIHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYEVNLIGGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQM
                     DGLETVLAAMCEPQIAIVSLTITEKGYFHSPATGQLMLDHPMVAADVQNPHQPKTATG
                     VIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAKAVDVKLAQWIEDNVTFP
                     STMVDRIVPAVTEDTLAKIEQLTGVGDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAE
                     LVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRYAAYGLMLQEQAP
                     TLKVQGVDLQDYANRLIERYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIF
                     GDDLPDNSLFTAKVTEAYLSLLAHGAKATVAKYSVK"
     gene            complement(39377..40660)
                     /gene="ydfJ"
                     /locus_tag="ACHKTW_001562"
                     /pseudo
     CDS             complement(39377..40660)
                     /gene="ydfJ"
                     /locus_tag="ACHKTW_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582800.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter YdfJ"
     gene            41264..41377
                     /gene="ynfT"
                     /locus_tag="ACHKTW_001563"
     CDS             41264..41377
                     /gene="ynfT"
                     /locus_tag="ACHKTW_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnaM/YnfT family protein"
                     /protein_id="HFO6462614.1"
                     /translation="MNSILIITSLLIIFSIFSHALIKLGIGISNNPDKTDV"
     gene            41446..>41672
                     /gene="ydfK"
                     /locus_tag="ACHKTW_001564"
     CDS             41446..>41672
                     /gene="ydfK"
                     /locus_tag="ACHKTW_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein YdfK"
                     /protein_id="HFO6462615.1"
                     /translation="MKSKDTLKWFPAQLPEVRIILGDAVVEVAKQGRPINTRTLLDYI
                     EGNIKKKSWLDNKELLQTAISVLKDNKNLNG"
BASE COUNT        11018 a        10308 c         9583 g        10763 t
ORIGIN      
        1 aaaatcgaag tttattaaac cccttttgca tccattagtg tctttaggaa tattcgctat
       61 aaaataaggg ttgtgcttaa tgctttaaga aaaatagcaa ttttcccttg aatatagtac
      121 tggtgatgga acgatgaatc tgcaagctgg cttttaacaa gccagctcta aaagaaggga
      181 aataagaata actatactca aaaactaaca gccacggtca tcatgatgtg gctgtcaatg
      241 aaactataac ccaggcgctt tccataacga cgtcgtcagt ccatgatcaa ccagccccag
      301 ctgatcctga taaactgcct gccacttatc gcgtgaatcc tgataaagtt catgcttttc
      361 cgggtctggt gtgtgcgtcc gttcccagcg aaccaggcgt tctccggttt ctgccattga
      421 tgaaaaaatt ccggcaccga cgccagctgc aatggcacat cctaatgcag tggcttcttt
      481 gaccaccgga atattgacgg gtaatcccga gacatcagcg agaatttgac tccataattt
      541 cccttttgaa cctccgcctg caaagactaa cgatgaagga tgaatattcg agaaatcagc
      601 aatttgctgc aagttacacg ctgatacaat cgccgcattt tcttccagcg cacggaacaa
      661 tgtcgctttg ttacatttat ccgggtcaat ggacaagtta ataaaggaag gcgcagcgtg
      721 ataccaggtt ttaaagcgca ttctgtcgga gaagatcggc attacgcccc acgacccagg
      781 cggcacccga ctggccatct cttccagcag cgtataggtg tcgatgccta aacgttcggc
      841 aatcagtttt tcttcggcac agaaagcatc gcggaaccag cgcatggtga gtccggtaaa
      901 aaagcttata gattcagctt gtaccatgcc aggaataaca tgaggattaa cgcgcacgtt
      961 catttctggg tctgtcaccg gcgcggctaa atttacaact tgctgccaga atgtgccgcc
     1021 aagaaccgcg gtttgtgccg gacgcacaac gcctaaccca aggcaaccaa gctgcacgtc
     1081 gcctcctcca acgaccaccg gagtgcccgc cttcagaccg cagagttccg ccgcttgtga
     1141 acttaccacg cccagcaatg tgccggtttc tttgacagga gaaagaatat cggcacgtag
     1201 gccagccata tccagcaatg caggtttcca gtcacgggtg gttagatcaa gaagtcccgt
     1261 ggtgccagcg ttagagggat ccaccgccag ttcgccgctg agcatatagg ccagccagtc
     1321 gctgatcatg gtgatggttg atgcctgacg gtaaatatcg gaacgatggt gcgccagcca
     1381 aagtaatctg gggatggcac ttaaagccag tgtttgtccg gtcgcgcgat aaacttcgtt
     1441 ttcaaaggta ttgttgtgca gttctttaag ttcgctaact tcgcgtgccg ctctggcatc
     1501 cacattggcg caggcccaga tcggggctcc ttcattatta tataaaacaa tgccttcacg
     1561 catcgaacat gccgaaacgg cagcgatata ctccggggct atgccggcgt tgtgcagcgc
     1621 ctggcgcata cactcacacg ccagttgcca gtttttgttg agatcaaatt ccatagaacc
     1681 aggaacgtcc ggtactgcca ggtgccgcca ctccgcctgt cccactgcta tttgattgcc
     1741 ttccaggtcg aatatcacag cccgaatact tccggtacct gcatccagcg ccatcaggta
     1801 atactttgat tctgaagggg taaagagtcg agccattaga ttatcctcgg cttatgcgtt
     1861 ctgtctgttc ctctatacgt tctccatcat tcccggtaat aaggtcatgc aaatttaact
     1921 acgtaaaatc gccgctgctg tgtcctgatc ggtaaccagt gcgttgatat aaccgccttt
     1981 cattgcagcg gcaattgctt cggctttatt ttctccccct gccacgccaa cccggacggg
     2041 tatggtcttc agcgagctta aaggtaagcc aatcagttcg ttatgtattt taatatccgt
     2101 gacaacgtca ccttttgcat caaaaaagta gcctaaaatg tcgccaaccg ccccttttcg
     2161 accaatcatt aactgttcgc cctggctgat ataaccggag cgaatgatcg tcgcgtcgtc
     2221 ctgttgactg acggccccaa tgccgacaat cgccacatcg gctgcttgcg cggctaacag
     2281 aacatctttg acgcaatttt cattttttag cgtacgggca atgtcagcgg aggatgcccg
     2341 caacggagcc ggaataatat tcacactgca cgccgcgtta agctgcccga tacccgtcat
     2401 ataagaaccg acgccaccgg agagcgtgac caggcgaatt tgctgtgacg aaataaaacc
     2461 acttaagcgt tgcagcgtat tcatggttgc ctcgccaaaa ccaatcgcca gcatctgttg
     2521 tggttgaagt aaactcatca acatatgcgc cgcgcctatc cccagtcgcc cgccgacatc
     2581 agcatccgca agcccaggga tcacccggac atgttgcagc gaaaactgac gacgtaattg
     2641 agtttcatat tccagacagc cttcaaagcg agaattaatc tgtacgcgaa taatgccgga
     2701 ctgatgccct ttctccagca agcgcgacac tttcaaacgg gtcaggccga gacgatcgct
     2761 gatctcgctc tgggtcagcc cgtcgtgata gtaaaaccac gcgatccgcg cgacctgttc
     2821 ttcttcacac attccctgtt ctgaaattgc cgaatcgttg attgtcataa ttcattcttc
     2881 actttgaaca tatttaaatc tttaatgcaa ttgttcagtt cttgcgcatt tatatctgtg
     2941 atggcagcca cagtttgact ctacgagcat gaacaaacgc aaccgtgaaa atcaaaatag
     3001 cataaattgt gatctattcg tcggaaatat gtgcaatgtc cacctaaggt tatgaacaaa
     3061 ttaaaagcag aaatacattt gttcaaaact cacctgcaaa actgaacggg ggaaatatgc
     3121 aaacgagtga tacccgcgcg ttaccgctac tttgcgcccg ctcggtttat aaacagtatt
     3181 caggggtcaa tgtcctgaaa ggcatcgatt ttacgttgca tcagggggag gtccacgccc
     3241 tgctcggcgg caatggtgcc ggtaaatcga cgttaatgaa gattattgcc ggtattaccc
     3301 ctgctgatag cggtacgctg gagattgggg gcaacaacta cgccagatta acgccagttc
     3361 atgctcatca gctgggcatt tatctcgttc cccaggaacc gctgcttttc ccaagcctgt
     3421 cgataaaaga aaacatcctg tttgggatgg caaaaaaaca gctctccatg caaaaaatga
     3481 agaacttgct ggcggcgctg ggctgccagt ttgatctgca tagtctggca ggatcgctgg
     3541 atgtcgccga tcgccaaatg gtggaaatcc tccgcgggct gatgcgcgac tcgcggattc
     3601 tgatcctcga tgaacctacc gcctcgctta cccctgcgga aaccgaacgc ttgtttagtc
     3661 gcttgcaaga gctgcttgct actggcgtgg gtattgtttt tatctcgcat aagctgccgg
     3721 aaattcgcca gattgccgat cgaattagcg tgatgcgcga cggaaccatc gccttaagcg
     3781 gcaaaaccag cgaactgtct accgacgaca ttattcaggc catcacccca gcggtacggg
     3841 aaaaatcgct ctctgccagc caaaaattat ggctggagtt acctggtaac cgcccacaac
     3901 atgctgccgg aacgccggtg ctgacactgg aaaatctgac cggcgaaggt ttcaggaatg
     3961 tcagcctgac gctcaatgcc ggagaaattc tgggcctggc tgggctggtg ggagccggac
     4021 gcacagaact ggccgagacg ctctatggtc tgcgtacttt gcgtggcgga cgcattatgc
     4081 tgaatggtaa agagatcaat aaattatcca ctggagaacg tttactgcgc ggtctggttt
     4141 atctgccgga agatcgccag tcatccggac tgaatctcga tgcttcgctg gcctggaacg
     4201 tctgcgccct tactcataac cttcgtggat tctgggcgaa aaccgcgaaa gataatgcca
     4261 ccctggaacg ttatcgtcgg gcgctgaata ttaaatttaa ccaaccggaa caagctgcac
     4321 ggacattatc cggtggcaac cagcaaaaaa tcctcattgc caaatgcctg gaagcttcgc
     4381 cgcaagtatt gattgtcgat gagccgacgc gcggcgtgga tgtctcggcc cgtaatgata
     4441 tctaccagct gttgcgcagc atcgccgcac aaaatgtggc tgtgctgctt atctcctccg
     4501 acctggaaga gatcgaactg atggcagatc gtgtgtatgt gatgcatcag ggcgaaattg
     4561 cccactctgc actgaccggg cgcgatatta atgtcgagac tattatgcgc gttgccttcg
     4621 gcgatagtca gcgtcaggag gcatcatgct gaagtttatt cagaacaacc gtgaaatcac
     4681 ggcattgctg gcggtggtgc tgctgtttgt attacccggt tttctcgacc gccagtattt
     4741 aagtgtgcaa acgctgacca tggtttatag cagcgcgcaa atcctgatcc tgctggcaat
     4801 gggcgcgacg ctggtaatgc ttacgcgcaa tattgatgtt tcagtgggtt cgattaccgg
     4861 aatgtgcgcg gtgctgttgg ggatgttact gaacgcagga tattcactac ctgttgcctg
     4921 tgtcgcgact ttactgcttg gtttgctcgc gggatttttc aacggtgtcc tggtcgcgtg
     4981 gctaaagatc cctgccattg ttgccaccct tggcacgtta gggttgtaca gaggcatcat
     5041 gttgctgtgg actggcggta aatggattga agggttaccc gccgaactga aacagctctc
     5101 cgcaccgctg ctgtttggcg tttccgcgat tggttggttg acgataattc tggtggcatt
     5161 tatggcctgg ctgctggcaa agacggcgtt tggacgcagt ttttatgcca cgggcgataa
     5221 tttacagggc gctcgtcaac tgggtgttcg tacggaagcc attcgcattg tggcattttc
     5281 gttgaacggc tgcatggcgg cactggcggg aattgtgttt gcttcgcaga ttggttttat
     5341 ccccaaccag accggtaccg ggctggagat gaaagcaatt gcagcctgcg tgctgggcgg
     5401 cattagtttg ctcggcggtt ccggtgcgat catcggtgcg gtactcggcg catggttcct
     5461 gacgcagatc gatagcgtac tggtgctgtt gcgcattccg gcatggtgga acgattttat
     5521 cgcgggcctc gtgctgctgg cggtgctggt gtttgatgga cgcctgcgtt gtgcgctgga
     5581 acgtaatcta cggcggcaaa aatatgcccg ctttatgacg ccaccgccat ccgttaaacc
     5641 cgcttcgtca ggtaaaaatc gggaggccgc ataatgcgta ttcgctacgg ttgggaactg
     5701 gctcttgccg cactgctcgt tattgagatt gtcgcatttg gtgcaattaa cccgcggatg
     5761 ttagatctca atatgttgct gttcagcacc agtgacttta tctgcattgg cattgtcgcc
     5821 ctaccgctga cgatggtgat tgtcagtggc gggatcgata tttcgtttgg ttcgaccatc
     5881 ggcctctgcg ccattgcatt gggcgtactg tttcaaagtg gtgtgccgat gccgctggcg
     5941 atactcctga ccttactgct cggcgcattg tgcgggctga tcaacgccgg attaattatc
     6001 tataccaaag ttaacccgct ggtgattacg cttggcacgc tgtatctgtt tgccggaagc
     6061 gctctgctgc tttccggtat ggccggagcg acggggtacg aaggtattgg tggattcccg
     6121 atggcgttta cagatttcgc taacctggat gtgctgggac tccccgttcc gctgattatc
     6181 ttcctgatat gtctcctcgt tttctggctc tggctgcata aaacccatgc cggacgtaat
     6241 gtgtttttga ttgggcaaag cccgcgcgtg gcgctttata gcgcgattcc agttaaccgc
     6301 accttatgtg cgctctatgc catgacgggg ctggcgtctg cggtcgccgc tgtgctgctg
     6361 gtgtcgtatt ttggttcagc acgttccgat ctcggtgcgt cgtttctgat gcccgccatc
     6421 accgccgtgg tgcttggcgg tgccaatatt tatggtggtt ccggttccat tatcggcacc
     6481 gccattgcgg ttttattagt gggatatttg caacaaggtt tgcaaatggc aggagtgcca
     6541 aatcaggtgt ccagcgccct ttccggtgcg ctacttatcg tcgttgtcgt aggtcgttcc
     6601 gttagcctgc atcgccagca aattaaagag tggctggcgc gtcgggccaa taacccattg
     6661 ccataaagga tatcttcatg acacttcatc gctttaagaa aatcgcctta cttagcgctc
     6721 ttggcattgc cgcaatctct atgaatgtgc aggccgcaga gcgtattgca tttattccca
     6781 aactggttgg cgtgggattt tttaccagcg gtggcaacgg cgcacaacaa gcgggtaaag
     6841 agctgagcgt tgatgtgacc tacgacgggc cgacagaacc cagtgtttct ggtcaggtac
     6901 agttgattaa taacttcgtc aatcaaggtt ataacgccat tatcgtttct gcggttacgc
     6961 ctgatggctt gtgtccggca ctgaaacgcg ccatgcaacg tggtgtgaga gtgctgacct
     7021 gggactctga tactaaaccg gagtgccgct cttactacat taatcaggga acgcccgccc
     7081 agttgggagg tatgttggtg gatatggcgg cgcgtcaggt gaataaagac aaagccaaag
     7141 tcgcgttttt ctactcaagc cccaccgtta cggaccaaaa ccagtgggtg aaagaagcga
     7201 aagcgaaaat cgccaaagag catcctggct gggaaattgt cactacgcag tttggctata
     7261 acgatgccac taaatcatta caaaccgcag aaggaatatt aaaagcgtat agcgatctcg
     7321 acgccattat cgcccccgac gccaacgccc tgcccgctgc cgcacaagcc gcagaaaacc
     7381 tgaaaaatga caaagttgcg attgtcggat tcagtacgcc aaacgtgatg cgtccatatg
     7441 tggaacgcgg cacggtgaaa gaatttggcc tgtgggatgt ggttcagcaa ggcaaaattt
     7501 cagtgtatgt cgcggatgca ttattgaaaa aaggatcaat gaaaacgggc gacaagctgg
     7561 atatccaggg cgtaggtcag gtggaagtct cgccaaacag cgttcagggc tatgactacg
     7621 aagcggatgg taatggcatc gtactgttac cggagcgcgt gatattcaac aaagagaata
     7681 tcggcaaata cgatttctga tgtgcattac ttaaccggag taagttatgg cagatttaga
     7741 cgatattaaa gatggtaaag attttcgtac cgatcaaccg caacaaaata tcccttttat
     7801 cctgaaaggt tgcggtgcgc tggattgggg aatgcagtca cgcttatcgc ggatatttaa
     7861 tccgaaaacg ggtaaaaccg tgatgctggc ctttgaccat ggttattttc aggggccgac
     7921 taccggactt gaacgcattg atataaatat cgccccgctg tttgaacatg ccgatgtatt
     7981 aatgtgtacg cgcggcattt tgcgcagcgt agttccccca gcgaccaata agccggtggt
     8041 actgcgggcg tcaggtgcga actctattct ggcggaatta agtaatgaag ccgtggcgtt
     8101 atcgatggat gatgccgtgc gcctgaacag ttgcgcggtg gcggcgcagg tttatatcgg
     8161 cagcgaatat gaacatcagt cgatcaaaaa tattattcag ctggtcgatg ccggaatgaa
     8221 agtgggaatg ccgaccatgg ccgtgactgg cgtgggcaaa gatatggtgc gcgatcagcg
     8281 ttatttctcg ctcgcgactc gaatcgccgc tgaaatgggg gcgcaaatta tcaaaaccta
     8341 ttatgtcgaa aaaggttttg aacggattgt tgccggatgt ccggtaccca ttgttattgc
     8401 tggcggtaaa aaattaccgg agcgcgaggc gctggaaatg tgctggcagg ctatcgatca
     8461 gggcgcttct ggtgtggata tggggcgtaa tattttccag tctgaccatc cggtggcgat
     8521 gatgaaagcc gtacaggcgg tggttcacca taacgaaacg gctgatcggg catatgaact
     8581 ctatctgagt gaaaaacagt aactgcggat ttaaggagaa gaattatgca cgtcacactg
     8641 gttgaaatta acgttcatga agacaagatt gacgagttta tcgaagtttt tcgccagaac
     8701 cacctgggct ctgtacagga agaaggcaat ttgcgcttcg atgtcttaca ggacccggaa
     8761 gtgaattcgc gcttttatat ctacgaagcc tataaagatg aagacgcagt ggcattccat
     8821 aaaaccacgc cccactacaa aacctgtgtc gcgaaactgg aatctttaat gaccgggccg
     8881 cgtaaaaaac gtctgttcaa tgggttgatg ccgtgaggcg aatttatcat ttttatctac
     8941 aattggggta acgcgctgac gggagtaaat aaatgtctga ctggaacccc tctttatatc
     9001 tgcactttgc cgctgaacga tcgcgtccgg cggtggagct gcttgccaga gtaccgctgg
     9061 agaatatcga atacgttgcc gatcttggct gtggcccagg taacagcacc gccctgctgc
     9121 atcaacgttg gcctgcggcc aggataacag gcatcgactc gtctccggcg atgattgctg
     9181 aagcgcgcag tgctttgcca gactgccagt tcgtggaagc agacatccgc aactggcaac
     9241 cggaacaggc actcgatctg atttttgcta atgcctcact gcaatggctg cccgaccact
     9301 acgaattgtt tcctcatctg gtttctttac ttaatccgca gggcgtgctg gcagtacaga
     9361 tgccagataa ctggctggag ccgacccatg tgctcatgcg cgaagttgcc tgggaacaaa
     9421 actacccgga tcgcgggcgt gagccgttgg ctggcgttca tgcttactac gatattttga
     9481 gcgaagccgg atgtgaggtc gatatctggc gaaccaccta ctatcaccag atgccgtcac
     9541 accaggcgat tatcgattgg gtgactgcca ctggattacg tccgtggtta caggatctga
     9601 ccgagagcga acagcagctt tttcttaagc gctaccatca gatgctggaa gagcagtatc
     9661 cactgcaaga gaacggacag atactgctgg catttccgcg tctttttatt gttgcccggc
     9721 gtatggagta aatatcacgt cagctggtaa tgacgatcgg gaagaatttt tgctggaatt
     9781 tcggcttcat cgttcatctg taacaggtca atttcaatag cgttgcagat ggcatccagc
     9841 ggtaaatcat tgttttcagt accgaacgga tcttccagtt cttccgccag acaatccagc
     9901 gaaataaaag tgtaggaaat cagcacagag ataaaaggcg tcatgtaatg caggtccacg
     9961 accagcgcga acggcagcat gatacaaaac agataaacgg tacgatgcaa aatcagggtg
    10021 taggcaaagg gaattggcgt ataggcaatg cgctcgcatc ctgccaggac cgctgaaata
    10081 tcattaagcc gatcgttgag gctaataaac aggatatctg aaagctgccc attgcggcgc
    10141 tgaaccgcca accactcccc cattattaac aagatacggt tagctggaga gttcgaagcc
    10201 agtacaggct gaagatcttc agtcttgaga taatgagcca gcacttccgc ctgtggctgt
    10261 ttgcgtaatg tcatgcgtaa acagtgggcg aaggcgattt gcaaccgggc aaactccctt
    10321 acacttgccg aatccggcaa tgtcgttttt acctcgcgca gtaacgaccg tgaggcaatc
    10381 atcaactgcc cccaaagttt tcgcgcttca acgtaacggg cgtacccggc attattacga
    10441 aaaccaagaa aaatggcgat ggcgacaccg agaatgctga acggtgcgag ggtgaatttg
    10501 atgcccagat gcgtgtacca gggcagcatg aaaataacag cgatagaaaa gagaaaattg
    10561 agtagtaagc gcgatgatat cttggataat actgagccgt gccagacaaa aatacggcgc
    10621 agccagtgtt gttgtggacg aacaatcatg gttatcttca ggcgtgaaaa agtcgcccta
    10681 ttaaacgtga ttacgatcac attctcaaga cgctacttac aaattaccta caagcttaca
    10741 acattaacag gcgctgcatg tggcatctgt taatgataaa ggcacatagg atgttgtaac
    10801 taatatagtg aaataagaaa cccggtcgaa accgggttca gaagtaacgg tgttattagc
    10861 acaacggacg tacagcttcg cgcatccctt tttcaagaat cgcatccagg tcattagcaa
    10921 cctgctcgac cagaccaggc acttgcgtca ggtcctgctc ccagtggtct ttttctgcca
    10981 acacaatcgc taccagttcc tgggtaccga taacacggtc acgatgctgg ctccagagtt
    11041 gctgataacg ttccagccag tgtgcatcgt cctgtaccgg atatgtttct ccgttacgct
    11101 caccacgata gaaggcaatc aatgccgcta atgcgaaagt aaggcgcgcc ggaagtgtgc
    11161 cttttgcctt ctgccctgcc agcagctgcg gcaggatgcg agtgcggaac ttggtcatac
    11221 cgttgagcgc gatagacagc agctgatgct taatgtacgg gttacggaaa cgcccggtga
    11281 ctgcactggc gaaagattcc agttcatcac gaggcaaatc cagtaccgga ataatttctt
    11341 cgtagatagc tttttcaacg aatgcgcaaa tttcagcatc gttcatcgct tcacctacgg
    11401 tatccagccc tgcctggaac gccaccggca ccagcgcggt gtgcgcaccg ttgaggattg
    11461 ccactttgcg ttctttgtac ggtttaatat cgtcaacaat cagcacgttg agcggatatt
    11521 tgtccagacg cagttcagtc gctaaggatt tcggcccctg aatcacaaac aggtaaaagt
    11581 gttcagcggt gtcgaggaaa ccatcgtgat aacccagttc ttcttccagt ttagccactt
    11641 catcgcgcgg ataaccggta acgatacggt ccaccagcgt agaacagaag ctgttagcct
    11701 gatccaacca ttgaataaat gcttctggca gtgcccactc ttgagcatag cgcagcacca
    11761 gttcacgcaa cgcgtcgcca ttgtagtcga tcaactcaca cgggatgatg atccaacctt
    11821 tatccagcgc accgttgaaa tggctaaaac gttcgaacag cagacgggtc agttttgcgg
    11881 gatagcttac cgctggcgcg tcatcgaatt tatcgcccgc atggtagctg atacccgctt
    11941 cggtggtgtt agagaaaaca aagcgcattt ccggattgtg cgccagtttc aggaattcat
    12001 cgtattcact gtagacgctg atttcacgat taacagagcg aatcagacgc gcgtcgctga
    12061 ccgcttcccc cttctcattc aggccacgga taatggtggt gtacagacca tcctgcgtgc
    12121 tcagtgacgg cgggaatgaa gtttcaatcg gacgaacaac gaccacgcca gaattcagat
    12181 cggtgtgctc attcaggaga tcgatttgcc agtcaacaaa ggcgcgcagg aagttacctt
    12241 caccaaactg aatgatacgt tctggatact gtgcaccggg aaaatcgcga cgatttagtg
    12301 ttttcacaat gggttccctt ctgattagtc atacaacctg cttgaattgg tacgacaggt
    12361 tagcaaactt taatacgccg aacccctgtt ttgatcaact cctgatgatt aatgagcagt
    12421 tttatgagaa aagtgtggcg cggatcatgg tttaatcgag gaaatacgcc ttttcctgga
    12481 tcataaagtg gtagaacaca ttgcattcaa atcgcgcgga atgaataaag atgtcagaca
    12541 acttcctcac cgtaacgcat agtgctggta cggttgcgcc catctttctt cgagcgatac
    12601 agacacgtat cggcttcgac catcaattta ttaaaatcat cggtcagcgt tcggtacgat
    12661 gcgcgaccac taccgacgcc aatacttacc gtgagataaa gcgttttttg ttgccaggtg
    12721 aatggttgca gttcaacgcc tttacggatt ttttccgcca ttagcagacc atcgacagga
    12781 ttcaccgacg gcactgcaac agcaaattct tcgccgccca tgcgcgccac cagcccctta
    12841 tcaccgacaa tcttctgaat atgccgggca aacacgctta acactttatc gccacattca
    12901 tgtccatagt tatcgttaat gcttttgaaa tagtcgatat caagcagcat gacagtcaga
    12961 tgttgtgtct gtttcagcga tggacttttc agcgcctcat aaagaccgga ccgtgagtaa
    13021 acctgagtca gaaaatcaaa gtcggctcgc agcgcaactt gcttcattag cgaattgatc
    13081 gctgccacgc taaaagaaac cataattggg catatcgcca tcgtggcaat accgagacgt
    13141 gcggagaaca tttgcggaat ggagaacggc gaaccgaccg agatatcaat caccgaattt
    13201 gccaccagta cgatttccac cgcaccggtg acaaaggtca acagacatgt tacctgcggc
    13261 gtatagcgca ctgcacacca gattaatgca ggcagcggga acgccagact ccccgctccg
    13321 ccaatgacta ccgaggcaat aaccgacaca ataagcgcga tggcgggcat catctgttct
    13381 gctttaaagc gcggcagcac tccaggaata gccaacgtca gcatacaagg cacgatcaaa
    13441 acgcccgttg agaattgctc actgaaccag tccgcaagca aaggccagaa atccagacta
    13501 tcaatactga ccgaaccaat cgccccgaca atagcgcata ataatgcggc aatcagacag
    13561 taattaaata gccgtaaggc acttactggc tcatacttat ttttccccaa acgcttatcc
    13621 cgcacgacca gtaaggcgac ggtaacaata aacatcatat tggagaaatt gatagccagt
    13681 gaaaccagtc cccattcggt ggttatggca tcataaacca gcatcgccac ataactgatc
    13741 gcatagtaat gcagtcgatt aagccagaca tagcgggcaa acacccccgc cattacgcca
    13801 ttcagcggcc agaaaagaga aagttcgtga accagccgta actctgcacc aataaaatag
    13861 aacagcgtgg ttagcgcaaa aatagcgatg ctattacgta gcagatgacc ttcctggaac
    13921 aaacggaaag ttgaaatggg gtgtacatgc attaattatc tgtccataca attgcgggtg
    13981 gcaattatct caacgtaata agtaaattct cataaaggat agcatgttcg ctgacagcgg
    14041 cggcaaataa aggtaccctg ggaatgctat tttatcgttt tccgcaattc tcatggctga
    14101 ttcatctgta caaccagcca gtgatagatt accgccacga tataacgttg ttgttcttcg
    14161 cttaacgaaa caccctgcgg aatgttatgc cacttagcca gacaaccgcg acaacaggtt
    14221 gcagtggcgt gctgagcgat aaacactgga tgcccgcgca tgggggtttg cttaccgtca
    14281 ttagccggta atgccggagc aagacgttta gcgacaaaat ccgccgcatg ttgttcgatg
    14341 actggcgcgc ctttctccag acaatactga cgctctttca tgcccaaacg aaagcgagat
    14401 ctaaattttg aacgggataa acgcgcgaag agtggatcga gagactgcat taataaaccg
    14461 aacgccctaa ctgtttcgtt aattgttcaa gaacggcaat gcccgcaagc gagttacctg
    14521 catcatccag ttccggactc cagactgcga tggccatttc atgcggaaca atcgccacaa
    14581 taccgccacc aacgccagat ttcgccggta gtcccacccg ccaggcaaac tcccccgcgt
    14641 tctggtacat accactggtc gccatcagcg cgttaatttg ccgcgcctgc attggcgtca
    14701 ccacaggttc atcaatatga atagctttcc cctgattagc cagaaagaca aacgtccggg
    14761 ccagctctac acagctcatt ttcagagcgc agtaatgaaa gtagttttgc agaacggttg
    14821 tcacgtcatg atggaaattg ccaaacgact tcatcagcca ggcgatagcc gcatttcgcg
    14881 cggaatgttc aaattcggaa cgcgctacca ccgtatcgta tgaaatatca gacacaccgc
    14941 ttaagccgcg cacgacttcc agcatacgtt gccgtggtgc gcttagtcgc ccttgcaaca
    15001 tatcgcagac caccagcgca ccggcattaa tgaacggatt acgcggaata ccctgttcca
    15061 tttccagttg cactaaagaa ttgaacggtg atccagacgg atctttgccg acgcgttgcc
    15121 agatttcctc ttcggagtaa tgacgcatgg cgacaacgag actcagcact ttggaaatag
    15181 actgaatgga aaaacgttct tgcgcgtctc cggcctgaaa aagctgtccg tcaacggtac
    15241 agatagcaat gcccaatcgg gaaccgtcta ctgtagccag cgccggaata taatccgcga
    15301 ctttaccctg accaatgagc ggccgcactt gccgcaagat gttttctaaa attgcattat
    15361 ccatggcgac tgccactttc tactcctgga ccgcaggtct gaaaagacct gcgagtatat
    15421 cagagctgaa tatgtcgcgt cagatccggt ctttccacac cgtctggata ttacagaatt
    15481 cgtgtaagcc gaaatgggaa agctcacgac caaagccact ctttttcacg ccaccaaagg
    15541 ccactcgcgc gtcgctggca caataaccat tgataaacac cccaccgcat tccagacgtg
    15601 ccgccatctg tctggcctgt gtttcgtcgg tggtaaaaat ggtcgctgaa aggccgaact
    15661 cactatcatt agccagttcc agcgcatgtt ctgcatcttt cgcaacggtg attgccgcaa
    15721 cagggccaaa catttcttcc cgaaacgcgg tcatttctgg ggtaacattc gccagaaccg
    15781 ttggcggata gtagttacct gccccagcca tcttttcccc gcccagtaac aaacgcgcac
    15841 cctgcgccag ggttttctcc acctgatgat gcagctcatc acgtaaatca aaacgagcca
    15901 ttggtccgag agcattctct tcatcacggg gatcgcccat tttcaaggct gccgcagctg
    15961 ccacaaaacg ttcggtaaat gcagaagcaa ttccctcttc gataataaag cgttttgccg
    16021 ctgcacaaac ctgtccggta ttctgataac gtccggctac cgccgcttta accgccagtt
    16081 ccagatcggc atcgttaagc acaataaatg gatccgaacc gcccagttcc agtacgcatt
    16141 ttttcagtgc cgctccagcc tgtgcgccaa tagccgctcc cgcacgaaca cttccggtca
    16201 ccgtgacagc agcaatgcgc gaatctttaa tcatttgact gacaccgtcg ttgtcggcat
    16261 tcagccagcc atatacgcct tgcgggattc ccgcatcttt aaacacctgg gcaatgagct
    16321 gagcacagcc catcacattc ggcgcatgtt taagtaagta gccgttacct gcaagaatga
    16381 tgggaaccgc gccacgcatc acctgccata acggaaagtt ccacggcata atcgccagaa
    16441 tcgtccccaa cggtcgatac tcaataactg cctgctgatt ttccaccagc gtaggttccg
    16501 ccttcagcat tgccggacca tgttctgcat accagtcaca caaattcgcc gatttcgcca
    16561 cttcagcgcg cgcctgattg attggtttgc ccatttcacg ggtgatcatt tgcgccattt
    16621 cttcgctacg ggcgcgcaga gccttaccga taccacgcag tttttcagca cgataatcta
    16681 tatttgtctc gcgccagtcg cgaaagcctg ctgccgccag ctgaagtgcg ttttcgatat
    16741 cgttagcgcc agcccaaggc agcacagaaa gttgttcacc cgtggcagga tttattgaaa
    16801 ttgcatgagt tgccggagta atggtcatcg gggtatctcc tttatgagtc atggtatgaa
    16861 gatacgcaga tttactcttg ctttaaaatg aataatatta agccacttat tcacgaatcg
    16921 agaatgttat ggatctgacc caactggaga tgttcaacgc ggttgccgaa gctggcagca
    16981 ttacccaggc tgcagcaaaa gtgcatcgcg tgccgtcgaa tctcactacc cgtctacgcc
    17041 agctggaaac agaactgggg gtagatctgt ttattcgcga gaatcagcgt ttacgtctct
    17101 ctcctgccgg gcataacttt ttacgctata gccaacaaat tctcgcgtta gtggatgaag
    17161 cgcggagcgt tgtcgctggc gatgagccgc aaggtttatt ttctcttggt tcgctggaaa
    17221 gcaccgcagc agtgcgcatt ccggccacgc tggcggaatt caaccatcgt tatcccaaaa
    17281 ttcagttttc actttccacc ggcccttccg gcactatgct ggaaggagtg ctggagggaa
    17341 aactgaatgc ggcatttatt gatgggccca ttaaccatac tgccattgac gggataccgg
    17401 tataccgcga ggagctgatg atcgtcacgc cacaaggtca tgcgccagta atccgcgcca
    17461 gtcaggttaa cggcagtaac atttatgcct tccgcgccaa ttgttcgtat cgtcgccact
    17521 tcgagagctg gtttcatgct gacggtgccg ctccgggaac tattcacgag atggagtcgt
    17581 atcacgggat gttggcctgt gtggtcgcag gagcaggcat tgcgcttatt ccacgctcta
    17641 tgctggaaag tatgccgggg catcaccagg ttgaagcgtg gccgttagct gagcaatggc
    17701 gttggttaac aacctggctg gtctggcgtc gtggtgcgaa aacgcgtccg cttgaggcat
    17761 ttattcaact actggatgtg cctgactcgg caaaacaggg ttaccaatga gctatttttg
    17821 atagttcttg cagctcaaat gccctatata gtgatgttcc gatgacttat gactatatgg
    17881 ggcaaatatg gttacgccag taagcatcag caattatata tctcttcctg atgattttcc
    17941 cgctcgtaat attgcacctc aggtaaaaga agttttaaag gattttattg atgcactcag
    18001 taccataata tgtgatgaag agtggcgcac gtctttaaac atcaactcag ccacaaaaaa
    18061 gatatttaat aaccttgata acttatcata tattcagagg acttctttcc ggggtaacga
    18121 cacgctatac aatgaaaaga ttcagtttaa actcacttat cccgtcaaga atggaagaca
    18181 caaagaaaat attgaatttc aggtactaat aaatttaagc cctatttatt tagataattt
    18241 tcgccatgat ggagaaatta atattttttg cgctcccaac ccaaagcctg tcactatggg
    18301 gcgcgtattt cagaccggcg tcgagcgtgt gccctttctg tttctgaatg attttattga
    18361 acaatttccg atgatcaacc ctggcgctcc catcaaaaga gcgcatacac cacatattga
    18421 acccctgcct ccggatcacc ataccgctgc agattactta cgccagtttg atttgcttgt
    18481 cctgaatttt atctctcgcg gtaattttgt catactcccc cgattatgga ataactctga
    18541 ggttcacaga tggttcgtca ataaagatcc taacttgaac accgccattc tcgacataac
    18601 ggacagcgaa ttaaaagagg atttattgca aagcctgatg gattcattag gttctaacaa
    18661 acatgtacta cccgaagtct gcatctgctt tttatccctt ttagcagaac aagaatctcc
    18721 tcattttcag gacttgtttt tatttttcgc caatatgtta ttgcactatc accaatttat
    18781 gaatcccaat gaaagtgatt tgaatgacgt gttgatgcca gcatcattaa gtgatgataa
    18841 aattatcaaa catatggcgc gcaggaccct caaacttttt gtaaaaaatg aaacgccgcc
    18901 aaaagttact cacgaagacc tggtgaaaaa caggcctcgc tcccctgtca gaccacctat
    18961 acccgcaacc tccaaaacgc cagacctccc tgaacgtcat taaaccgtga tgttaccgac
    19021 tctctgacgc gtgaaagaat cagcgtcaga gaaacggaaa acgcgatcca gatcacaaat
    19081 gcattgtatt cacatcatta accgttttaa gatcatttca tcactttttc gcaactcacc
    19141 cgataatctg ttatgacaac aaacactgtt tcccgcaaag tggcgtggct acgggtcgtt
    19201 acgctggcag tcgccgcctt catcttcaac accaccgaat ttgtccctgt tggcctgctc
    19261 tctgacattg cgcaaagttt tcacatgcaa accgctcagg tcggcatcat gttgaccatt
    19321 tacgcatggg tagtagcgct aatgtcattg ccttttatgt taatgaccag tcaggttgaa
    19381 cggcgcaaat tactgatctg cctgtttgtg gtgtttattg ccagccacgt actgtcgttt
    19441 ttgtcgtgga gctttaccgt tctggtgatc agtcgcattg gtgtggcttt tgcacatgcg
    19501 attttctggt cgattacggc gtctctggcg atccgtatgg ctccggccgg gaagcgagca
    19561 caggcattga gtttaattgc caccggtaca gcactggcga tggtcttagg tttacctctc
    19621 gggcgcattg tgggccagta tttcggttgg cgaatgacct tcttcgcgat tggtattggg
    19681 gcgcttatca cccttttgtg cctgattaag ttacttccct tactgcccag tgagcattcc
    19741 ggttcactga aaagcctccc gctattgttc cgccgcccgg cattgatgag catttatttg
    19801 ttaactgtgg tggttgtcac cgcccattac acggcataca gctatatcga gccttttgta
    19861 caaaacattg cgggattcag cgccaacttt gccacggcat tactgttatt acttggtggt
    19921 gcgggcatta ttggcagcgt gattttcggt aaactgggta atcagtatgc gtctgcgtta
    19981 gtgagtacgg cgattgcgct gttgctggtg tgcctggcat tgctgttacc tgcggcgaac
    20041 agtgaaatac acctcggggt gctgagtatt ttctggggga tcgcgatgat gatcatcggg
    20101 cttggtatgc aggttaaagt gctggcgctg gcaccagatg ctaccgacgt cgcgatggcg
    20161 ctattctccg ggatatttaa tattggaatc ggggcgggtg cgttggtagg taatcaggtg
    20221 agtttgcact ggtcaatgtc gatgattggt tatgtgggcg cggtgcctgc ttttgccgcg
    20281 ttaatttggt caatcattat atttcgccgc tggccagtga cactcgaaga acagacgcaa
    20341 tagttgaaag gcccattcgg gcctttttta atggtacgtt ttaatgattt ccaggatgcc
    20401 gttaataata aactgcacac ccatacatac cagcaggaat cccatcagac gggagatcgc
    20461 ttcaatgcca cccttgccca ccagccgcat aattgcgccg gagctgcgta ggcttcccca
    20521 caaaataacc gccaccagga aaaagatcag cggcggcgca accatcagta cccaatcagc
    20581 gaaggttgaa ctctgacgca ctgtggacgc cgagctaata atcatcgcta tggttcccgg
    20641 accagcggta cttggcattg ctagcggcac aaaggcaata ttggcactgg gttcatcttc
    20701 cagctcttcc gacttgcttt tcgcctccgg tgaatcaatc gctttctgtt gcggaaagag
    20761 catccgaaaa ccgataaacg cgacgattaa gccgcctgca attcgcagac cgggaatcga
    20821 aatgccaaat gtatccatca ccagttgccc ggcgtaatac gccaccatca tgatggcaaa
    20881 tacgtacacc gaggccatca acgactgacg attacgttcg gcactgttca tgttgcctgc
    20941 caggccaaga aataacgcga cagttgttaa tgggttagct aacggcagta acaccaccag
    21001 ccccaggcca attgctttaa acaaatctaa cattggtggt tgttatcctg tgtatctggg
    21061 ttatcagcga aaagtataag gggtaaacaa ggataaagtg tcactcttta gctaaccttg
    21121 catcgcattg accaaaactt gaaccgattt agcaaaacgt ggcatcggtc aattcattta
    21181 tttgacttat acttgcctgg gcaatattat cccctgcaac taattacttg ccagggcaac
    21241 taatgtgaaa agtaccagcg atctgttcaa tgaaattatt ccattgggtc gcttaatcca
    21301 tatggttaat cagaagaaag atcgtctgct taacgagtat ctgtctccgc tggatattac
    21361 cgcggcacag tttaaggtgc tctgctctat ccgctgcgcg gcgtgtatta ctccggttga
    21421 actgaaaaaa gtgttgtctg tcgaccttgg agcactgacc cgtatgctgg atcgcctggt
    21481 ctgtaaaggc tgggttgaaa ggttgccgaa cccgaatgat aagcgcggcg tactggtaaa
    21541 acttaccacc agcggcgcgg caatatgcga acaatgccat caattagttg gccaggacct
    21601 gcatcaagaa ttaacaaaaa acctgacggc ggacgaagtg gcaacacttg agcatttgct
    21661 taagaaagtc ctgccgtaaa caaaaaagag gtatgacgat gtccagacgc aatactgacg
    21721 ctattaccat tcatagcatt ttggactgga tcgaggacaa cctggaatcg ccactgtcac
    21781 tggagaaagt gtcagagcgt tcaggttact ctaaatggca cctgcaacgg atgtttaaaa
    21841 aagaaaccgg tcattcatta ggccaataca tccgcagccg taagatgacg gaaatcgcgc
    21901 aaaagctgaa ggaaagtaac gagccgatac tctatctggc agaacgatat ggcttcgagt
    21961 cgcaacaaac tctgacccga accttcaaaa attactttga tgttccgccg cataaatacc
    22021 ggatgaccaa tatgcagggc gaatcgcgct ttttacatcc attaaatcat tacaacagct
    22081 agttgataac gtgacaacgt cactgaggca atcatgaaac cactttcatc cgcaatagca
    22141 gctgcgctta ttctcttttc cgcgcagggc gttgcggaac aaaccacgca gccagttgtt
    22201 acttcttgtg ccaatgtcgt ggttgttccc ccatcgcagg aacaaccacc gtttgattta
    22261 aatcacatgg gtactggcag tgataagtcg gatgcgctcg gcgtgcccta ttataatcaa
    22321 cacgctatgt agttattgtc aggctctgat cacagagcct gacatctccg taatatcttc
    22381 ctcaagtctc aaatattttc gccattgttg gcgatgagat cccgatacca gtaaaacgac
    22441 gctttacgta tccgttccag cgttcccttc ccttcgttgt ctttatcgac ataaatcatt
    22501 ccgtagcgct ttttcatttc ccctgttccg gcagaaacca ggtcaatgca gccccacggg
    22561 gtgtagccaa ttaagtcaac accatcttca actacggctt ttttcatttc ccgaatatgg
    22621 gaagcaaagt aatcaatgcg atagtgatcg ttcaccgtgc cgtcagcttg tctctggtca
    22681 accgcaccaa atccattttc gacaataaac agcggcaact ggaaatgatc ccagaaccag
    22741 ttgagtgaat aacgtagccc tgccggatca atttgccatc cccattcgga tgtatcgata
    22801 taagggttgc tgaccaaatc tcgtggttca acataatcca gttgcgggtt atcgggcgaa
    22861 aattgtgtcg taaaagacat gtagtagcta aagccgataa agtcgacaca accactggca
    22921 agaatcgcgt tatcttctgg tgtgatatcg aggttgaatc ctttcctggc aaagtaattc
    22981 agcatatgtt gcggataata tccacgagca tgaacatcag taaaccagta acgacgatgc
    23041 attgctttcg tggccatcat catatcgttg ggtgcacacg tcagaggata gatgggacac
    23101 atggcgatca tacagccaat gttaaattca ggattgatcg attttccagt ctgtaccgcc
    23161 agggcactgg caactaactc gtaatgtacc gcctggtaca ttatttgttc gcgctcattg
    23221 atatcttcct ctggcgaata caagataccg gaattagtaa atggacacag gctttcgctg
    23281 aaattcacct gattattgat ttcgttaaac gtcatccagt actttacttt ttctttatag
    23341 cgcgtgaaga ccgttgatgc gaagcggata aaaaagtcga tcagtttacg gtttcgccag
    23401 ccaccatatt ttgtcaccag atgataaggc atctcaaaat gtgaaagcgt caccacaggt
    23461 tccattccct gcttaaggca ttcatcgaac agatcatcat aaaattgtaa accctcttca
    23521 ttcggctcct gttcgtcacc ttgcggaaag attcgtgtcc aggcaatgga agttcgaaag
    23581 catttgaatc ccatctcggc aaataactga atatctgttt tatagcgatg ataaaaatca
    23641 attgcttcat gattgggata attaagcccg tcgataacgc cttctgtcac ttcacgcggc
    23701 accccgtgag cgccagcagt catcacatca gcgatgctga tgccttttcc tccttcattc
    23761 cagccacctt ccaactgatg cgcggctacc gcaccgcccc ataaaaaacc ttttttaaat
    23821 cctgacatac acaactcctt aactaaaata gattaccacc agacttccgc ctggatgccg
    23881 gccataaact cgtctttgct gttctcatta aacttaaaat tcgataattc gttatccaga
    23941 atattgatat acgttccata gaagcgaatt tctggtcgtg aacctaaaat gctttcaccc
    24001 actttcaatg catgccagag tgtcgtttta taagccgatt catttagtgt tacaccctgc
    24061 tgagttttgt tctgttgctt aaaccagcct aattcaagcc ccgtctgatt ccatgtattc
    24121 cagatccagg ccggtcttat tacagtgcga atactgtcaa aatcactatg agcaccactt
    24181 tcataactat aaacatcttc gccgtgagaa tagacaagcg cgttagccat aataatattg
    24241 tcagtaagat acatctcgcc ctgagagatt aaacgccagg cgatcccatt ggtatgatca
    24301 ccatagtaat agcgaccatg cgccatcgta ttactggcat ctgagaaact ggcaaaactg
    24361 ctggcatagg aattattggc aacctgtaac gtaaattcat taaacgtatc gcgttgcagg
    24421 ttttgccgta aaacagaagt cagcatccag ctatctttca tttcaaaata actgttgtca
    24481 ttttcgttat cttgttgttg atccgtttta ttaggtgcgg aatatttagc cattaatgat
    24541 aatgtcgcat catcccataa cgggatattg cgataacgaa catccacaga attggtattc
    24601 atctggctgg tacgcgaaaa atcacgagag taaacatcga catcatcacg gcttaaggac
    24661 atatcaaaca gccctacacc aagtgcccag ttttcaatcc ccacacccgc ggcgacatcc
    24721 gtggttaagg ttttccagtc cagcatctgg atctcatatt gcgggagttt atgtttgccg
    24781 acccagaagt ctgcctctgg tgcgaagggt aaaaaacctc gcgttgtcag gtaaatatcg
    24841 ctgaactgca tgatattttc attggcactg tcaccaaacc aggcatcgtt atactgctca
    24901 cccacgttgc catcataggt aacgaccgca tttgccgttt taccgtcctg attataaaca
    24961 cgctgattta aggtcaggtc aaaccaaccg ctcatctcat taccaaaacg tcccagagaa
    25021 cccgctgcat aagtttgtgg tgagccgtga ttaccagtac cccagcctga gcggaagtac
    25081 ccctgatagc tgaacccaat atcatccttt atatatttac tgatatcttt cagggtcacg
    25141 ctttcgatag tggttttgtc gcctttactt tcaataacgg caacttgctg cgatccattt
    25201 atagtatgcg ctgcggcaga cgagttctgt tcaccgtttt catttttgat gttatcagcg
    25261 acgggagatg tcgctgatat ttcagcaagc gaggctgatt tatatttgtt ttccgtgatg
    25321 cttttttgta aggtcgaaag tcgggattta tatactccca gttcattctg tgttgctttc
    25381 agctcttgtt tattttgcga caattcattt tcaagcaatt caaggcgctg ctctatggtt
    25441 aattttgttg ccatcgcgtt caatggaaac aatgctgtaa taatagaaaa agacaaaagg
    25501 ctgacgttaa gcgtctttat attcatgact tatccttaag agtataataa tttcctccag
    25561 ctatcgcact ggaggaaaaa atcaggcatg gagttctttt cttaatgaaa taatttccct
    25621 ggctaaatcc tggcataata ttgcattcat caaatgatcc tgtgcatgaa tcagaattaa
    25681 attaactttt attttgccac agccttcatc ctgactaatt aatgcggtct gaatcttatg
    25741 tgcctgaaga caggcagatt cagaactggc cattagctga gttgcgccct gccagtcttt
    25801 ttgcccggca caacgtatgg cttccatcgc gtcagaacgt gcctgccccg cgttgaccag
    25861 taactccata acaagttctt catctgcaaa catcacaatc tccttcctgg gtaaatggaa
    25921 ttatccgtta gttgtcatgt tattgacttt gttggcggca ataacaaaag gtaaatagat
    25981 cattgtcgcc acacctaaac ataaaaaacc aaccagtaaa gccagccagt tccccccggt
    26041 tccgaggaaa gcaattaaag gtccgggcgt agtccagggc acggcataag caaccggtgg
    26101 aataatttcc atcgaaacaa agaagtaacc aatggcacag ttaacaatgg gaaccataat
    26161 aaacggcacc atcatgatgg ggttaagtac aataggaagg ccaaacatta tcggttcatt
    26221 gatattgaaa ataccgggga taaatgacat ttttgccaga tcacggtatt ccgcacgctt
    26281 agaagcgata aaaatagcca acaataaccc caacgtcata cctgaaccgc cgcagttggc
    26341 gaatgcatca ttaatagaag tccaggtaat cggatatggc gcgccccagg tagtgccatg
    26401 ttgagcggcc caggagagat tctccagatt agcctcagaa aaaacggttt ctcgaattgc
    26461 cgatgtggta ttaggtccgt ggatccccag cacccacagg aaattaccca cagcccccag
    26521 gataattacc gcaaagatat tcgttcccat atgtttgagc ggcgtctgga ttaatgtgta
    26581 aataagatcg tttagtcctg ccggggatat cagtgtcagg caataattaa gtgcggaaaa
    26641 gaacaccatg acaaaaaata ttggcaataa aactttaaat gaacgcgcta ccgcaggcgg
    26701 tacagctgcc ggcatcgtta tggtgatttt tgggtttcga gctaagcgac aaaatatttc
    26761 actggtgatc aatgcaacga taacagcaac aaacaacccc tgcgcgccga gatatttggt
    26821 cgtcaggaat gcttgcccat ctatcatctg atatggcgta taaacaataa aaaatgctcc
    26881 tattgccgtt aatccgcata aaagatcatc ttgctcatag ctaatcgcca tattcctggc
    26941 gaccaggaaa gcaatcataa ttgacatgat atttacagaa ccattcatta ccggagtaaa
    27001 aatagcttgt gctttttcaa ggttggggaa aatgctattc agatgcagta atccggcaat
    27061 aaaaccgtct ggcgataata tggcaaagtt aattaatata attaatgaac ttgccatgat
    27121 aattggaaac gtaaaaacga atccatctct caccgctgca acatgttttt gtgagtttaa
    27181 cttgacagca actggaacaa gaaaacgttc cattccacgt tcgaatgagg ccattaatcc
    27241 cattttttta tacctgttgt tttggatatt tatacatccc aataaagcat gtatgccata
    27301 tccattttat gatgtgtgaa aagttagttc aggtaatagt ttaataatat tagattctta
    27361 ctcttgcagt agtttcatcg caaattcaag aactgcctgt ccgttcatgg tgccgtagtc
    27421 tttcatctcg ataacggcta caggcatttt cccctgtgca acggcttcaa tctctggttt
    27481 ctgaaagcga acttgcgggc caagtaaaac aacgtcaact tcgttatttt taattttccc
    27541 tttagcctct gcaatcgcca atgcggaaat attaacttca agtgaaatag cgttagcatg
    27601 atcaatcata cgtttaacca gcatactggt tgacatgccc gcagcgcaaa ctaacaatat
    27661 ttttttcata ttttccctta ctggtatata acagattaca ttgtttaaat atcatatccc
    27721 gcagtagcta tacgtcctgg tacattgatc acagtaatac agtgataaag ttgttcaaca
    27781 tcagggtgat tattaaacga ctcggccata cgataaacca atcaggactc aactccttgt
    27841 tttcaatggc tcttaaaagg tgcaaaagtg atttttcaaa agattgcccg cttacttaaa
    27901 tccgaaatca atggcaattc atggcatgtt ggtgatttgt tgccgtcaga agcggaactc
    27961 gctgttcgtt ataatgtttc gcgtaatacc ctgcgtaagg cattatcctt gctggaaggc
    28021 gaaggaatta ttcacagaaa gcatggttca ggaacataca ttcaaaaaaa gaattttgtt
    28081 gcacacattg atcacatgaa tagtttcagt gaaattgcgc ataaaagcgg caaagaggca
    28141 gggagtcaga ttatgaaatt tgaagtgcag gatgcttcac ccactattgc gactgagctg
    28201 aatttagtga ctggtgagca ggtttattac ataaagagac tgcgattcat tgaggataat
    28261 gcagcacaat tagaagaaac gtggatgtca gtagcacgtt ttcctgattt aaccgtgtca
    28321 catatgcaaa aatccaaatt ttcatatatt gagaatgaat gcgggatcaa aatcattggc
    28381 acctttgaaa ctttctcccc tacttttcct accccagaaa tcgccagtat tttacggatc
    28441 agcccacgag atcccatact taaaattcag acccaggctg tggacagtaa ctctattccg
    28501 ctggattatt cgttacttta cagcaatatt ttcgagttcc aggtaaagta ctttttcccg
    28561 cgataaaagc agacgcacag agtgctgaca tcacattctg tgcgtatggt ttagcctcta
    28621 acctttaccg ctttacgcca ccgcaagcca aatacattga tatacagccc ggtcataatg
    28681 agcaccgcac cgaaaaattg tagacccgtt aagcgttcat ccaacaataa tgccgcactt
    28741 gccagtccga ctacgggcac cagtaacgat aacggtgcaa cccgccaggt ttcatagcgt
    28801 ccaagtagtg ttccccagat cccataacca acaattgtcg ccacaaacgc cagatacatc
    28861 agagacaaaa tggtggtcat atcgatagta accagactgt gaatcatggt tgcggaacca
    28921 tcgagaatca gcgaggcaac aaagaaggga atgattggga ttaatgcgct ccagattacc
    28981 agcgacatca ccgccggacg cgttgagtgc gacatgatct ttttattgaa gatgttgcca
    29041 cacgcccaac taaatgctgc cgccagggtc aacataaagc cgagcatcgc cacatgctga
    29101 ccgttcagac tatcttcgat taacaccagt acgccaaaaa tcgctaaggc gatccccgcc
    29161 aattgtttgc catgcaggcg ctccccgaaa gtaaacgcgc caagcacgat agtaaaaaac
    29221 gcctgtgcct gtaacaccag cgaagccagt ccagcaggca taccaaaatt aatggcgcag
    29281 aacagaaagg caaactgcgc aaaactgatg gttaatccat accccagcag caaattcagt
    29341 ggtactttcg gtcgtgcgac aaaaaagata gccggaaaag cgaccagcat aaagcgcaaa
    29401 ccggccagca tcagcggtgg catgttatga agccccactt tgatgaccac aaaatttagc
    29461 ccccatacga ccactaccag tagcgccaac accccatctt ttcgcgacat tctaccgcct
    29521 ctgaatttca tcttttgtaa gcaatcaact tagctgaatt tacttttctt taacagttga
    29581 ttcgttagtc gccggttacg acggcattaa tgcgcaaata agtcgctata cttcggattt
    29641 ttgccatgct atttctttac atctctaaac aaaacataac gaaacgcact gccggacaga
    29701 caaatgaact tatccctacg acgctctacc agcgcccttc ttgcctcgtc gttgttatta
    29761 accatcggac gcggcgctac gctgccattt atgaccattt acttgagtcg ccagtacagc
    29821 ctgagtgtcg atctaatcgg ttatgcgatg acaattgcgc tcactattgg cgtcgttttt
    29881 agcctcggtt ttggtatcct ggcggataag ttcgacaaga aacgctatat gttactggca
    29941 attaccgcct tcgccagcgg ttttattgcc attcctttag tgaataacgt gacgctggtg
    30001 gtgctctttt ttgcccttat taactgcgcc tattccgttt ttgctaccgt gttgaaagcc
    30061 tggtttgccg acaatctttc gtccaccagc aaaacgaaaa tcttctcaat caactacacc
    30121 atgctaaaca ttggctggac catcggtccg ccgctcggca cgctgttggt gatgcagagc
    30181 atcaatctgc ccttttggct ggcagctatc tgttccgcgt ttcccatgct tttcattcaa
    30241 atttgggtaa agcgcagcga gaaaatcatc gccacggaaa caggcagtgt ctggtcgccg
    30301 aaagttttat tacaagataa agcactgttg tggtttacct gctctggttt tctggcttcc
    30361 tttgttagcg gcgcatttgc ttcatgcatt tcacaatatg tgatggtgat tgctgatggg
    30421 gattttgccg aaaaggtggt cgcggttgtt cttccggtta atgctgccat ggtagttacg
    30481 ttgcaatatt ccgtgggccg ccgccttaac ccggttaaca tccgcgcgct gatgatagca
    30541 ggcaccctct gtttcgtcat cggtctggtt ggttttattt tttccggcaa cagcctgcta
    30601 ttgtggggta tgtcagctgc ggtatttact gtcggtgaaa tcatttatgc gccgggcgag
    30661 tatatgttga ttgaccatat tgcgccgcca ggaatgaaag ccagctattt ttctgcccag
    30721 tctttaggct ggcttggtgc tgcaattaac ccattagtga gtggcgtagt gctaaccagc
    30781 ctgccgcctt cctcgctgtt tatcatctta gcgttggtga tcattgctgc gtgggtgctg
    30841 atgttaaaag ggattcgtgc aaaaccgtgg gggcagcccg cgctttgttg atttaagtcg
    30901 aacacaataa aaatttaatt cagccttcgt ttaggttacc tctgctaata tctttctcat
    30961 tgagatgaaa attaaggtaa gcgaggaaac acaccacacc ataaacggag gcaaataatg
    31021 ctgggtaata tgaatgtttt tatggccgta ctgggaataa ttttattttc tggttttctg
    31081 gccgcgtatt tcagccacaa atgggatgac taatgaacgg agataatccc tcacctaacc
    31141 ggccccttgt tacagttgtg tacaaggggc ctgattttta tgacggcgaa aaaaaaccgc
    31201 cagtaaaccg gcggtgaatg cttgcatgga tagatttgtg ttttgctttt acgctaacag
    31261 gcattttcct gcactgataa cggatcgttg acacagtagc atcagttttc tcaatgaatg
    31321 ttaaacggag cttaaactcg gttaatcaca ttttgttcat caataaacat gcagcgattt
    31381 cttccggttt gcttaccctc atacattgcc cggtctgctc ttccaatgac cacatccaga
    31441 ggctcttcag gaaatgcgcg actgacacct gctgtcacgg taatgttgat atgcccttca
    31501 gaaagcgtga tggcatggtt atcgactaac tggcaaattc tgacacctgc acgacatgct
    31561 tcttcatcat tagccgcttt gacaataatg ataaattctt cgcccccgta gcgataaacc
    31621 gtttcgtaat cacgcgtcca actggctaag taagttgcca gggtgcgtaa tactacatcg
    31681 ccgattaaat gcccgtaggt atcattaacc aatttaaatc ggtcaatatc caacaacatt
    31741 aaataaagat tcagaggctc agcgttgcgt aactgatgat caaaggattc atcaagaacc
    31801 cgacgccccg gcaatcccgt caaaacatcc atattgctac ggatcgtcag caaataaatt
    31861 ttgtaatcgg ttaatgccgc agtaaaagaa agcaacccct cctgaaaggc gtcgaaatgc
    31921 gcgtcctgcc agtgattttc aacaatagcc agcattaatt cccgaccaca gttatgcata
    31981 tgttgatggg cagaatccat tagccgaacg taaggtaatt catcgttatc gagtggcccc
    32041 agatgatcaa tccaccgacc aaactggcac agtccataag aatggttatc cgttatttct
    32101 ggcttactgg catctctcgc gaccacgctg tgaaacatac tcaccagcca ctggtagtgg
    32161 gcatcgatag ccttattgag atttaacaag atggcatcaa tttccgttgt tttcttgatc
    32221 attgccactc ctttttcaca gttccttgtg cgcgctattc taacgagaga aaagcaaaat
    32281 tacgtcaata ttttcataga aatccgaagt tatgagtcat ctctgagata acattgtgat
    32341 ttaaaacaaa atcagcggat aaaaaagtgt ttaattctgt aaattacctc tgcattatcg
    32401 taaataaaag gatgacaaat agcataaccc aataccctaa tggcccagta gttcaggcca
    32461 tcaggctaat ttatttttat ttctgcaaat gagtgacccg aacgacggcc ggcgcgcttt
    32521 tcttatccag actgccgcta atgttgatca tctggtccgg ctgaacttct cgtccatcaa
    32581 agacggccgc aggaataacg acattaattt caccgctctt atcgcgaaaa acgtaacggt
    32641 cctctccttt gtgagaaatc aaattaccgc gtagtgaaac cgaagcgcca tcgtgcatgg
    32701 tatttgcgaa atcaacggtc attttttttg catcatcggt tccgcgatag ccatcttcta
    32761 ttgcatgagg cggcggtggc gctgcatcct gttttaaacc gccctggtca tctgccaacg
    32821 cataaggcat gacaagaaaa cttgctaata caatggcctg aaatttcata ctgactcctt
    32881 aattgcgttt ggtttgactt attaagtctg gttgctattt ttataattgc caaataagaa
    32941 tactgccaat tgttataagg catttaaaat caggcaacta gctgtcaaat attcagagaa
    33001 tttaactcac taaagttaag aagatcaaat agtcttaaat atattttcag aataatcgga
    33061 tttatatgtt tgaaaattat tatattggac gagcatacag aaaaagcaaa tcacctttac
    33121 atataaaagc gtggacaaaa aacagtgaac attaatagcg ataaaattgt acaacttgca
    33181 gataccgaca ctattgaaaa cctgacatcc gcgttgagtc aaagacttat cgcggatcaa
    33241 ttacgcttaa ctaccgccga atcatgcacc ggcggtaagt tggctagcgc cctgtgtgca
    33301 gctgaagata cacccaaatt ttacggtgca ggctttgtta ctttcaccga tcaggcaaag
    33361 atgaaaatcc tcagcgtaag ccagcaatct cttgaacgat attctgcggt gagtgagaaa
    33421 gtggcagcag aaatggcaac cggtgccata gagcgtgcgg atgctgatgt cagtattgcc
    33481 attaccggct acggcggacc ggagggcggt gaagatggta cgccagcggg taccgtctgg
    33541 ttcgcgtggc atattaaagg ccagacctat actgcggtta tgcattttgc tggcgactgc
    33601 gaaacggtat tagctttagc ggtgaggttt gccctcgccc agctgctgca attactgcta
    33661 taaccaggct ggcctggcga tatctccagg acagccattg gtggtgttta tatgttcaag
    33721 ccacgatgtt gcagcatcgg cataatctga ggagccttac cgcgccattg tcgatacagg
    33781 cgttccagat cttcgctgtt acctctggaa aggatcgcct cgcgaaaacg cagcccattt
    33841 tcacgcgtta atccgccctg ctcaacaaac cactgataac catcatcggc caacatttgt
    33901 gtccacagat aagcgtaata acctgcggca tatccgccac caaaaatatg ggcgaaataa
    33961 ctgctgcgat agcgtggcgg tatagcagga agatccatat tttccgccac cagcgcccgc
    34021 aattcaaaat catcgacatc ctgcattgct tcgttttctt ccaggcaatg ccagcgcata
    34081 tcgagaagtg cggcgctaag cagttcgctc atctcatacc ctttgttgaa caggctggca
    34141 ttacgcattt tctgttgcag ttcgtcaggc attgctgccc cgctctgata atgccgggcg
    34201 tagcgagcga atacctgcgg atgcgttgcc cagtgttcgt tgatttgcga cggaaattcg
    34261 acaaaatcac gcggcgtgtt ggtgccggaa agcgtggcat aacgctggcg ggcaaaaagg
    34321 ccgtgcagcg tatgaccaaa ttcatggaat aaggttatga catcatccca gagtaacaac
    34381 gcaggctcac cggcagcggg tttctgataa ttgcaaacgt tataaattac cggatgcgtt
    34441 tcattaagcg ttgattgctc aacaaaattg cccatccatg caccgccgct ttttgaatca
    34501 cgggcgaaga aatcaccgta aaataacgcc agtcccacgc cattatgatc aaaaatttcc
    34561 cacacacgaa cgtcaggatg gtagacagga atatcaaaac gttcgacaaa cttaataccg
    34621 aagagctgat tcgcggtcca gaatacacct tcattcaaca ccgtgtttaa ttcaaaatat
    34681 ggcttgagct gcgcctcatc aagatcaaat ttctcccgcc gtacctgttc ggcataaaat
    34741 gcccagtccc acggctgcgc gctaaacccg ccttgctgct tatcgataac cgcctgtatg
    34801 gaggctaatt catcgctagc acgttgacgc gccgctggaa caatttcccg cataaagttg
    34861 agtgctgctt caggtgtttt tgccatctga tcggcgattt tccatgcggc ataatgagga
    34921 aaaccaagca gtttcgcctg ctgtgcgcga atctctacca gacgttgaat gatagcgcgg
    34981 gtatcattgc catcattttt ttccgctcgc gtccagcccg caataaacag tttttcacgc
    35041 gtcgcacgat cgcgcatttc ggcaagcgcc ggttgttggg tggtattcag cagcggaatc
    35101 agccatttgt tatccagacc tttctcgcga gccgcctctg ccgccagcgc aatctcttgc
    35161 tcactcattc ctgccagctg cgcgatatcg ttcacaacca gaccgccgga tttatttgct
    35221 gcaagtaacc gctggttaaa ctggctggtc agggtcgcag cttctgtatt cagtactttt
    35281 aattttgctt tatcagcttg cgcaagtttg gctccggcaa ggacaaaacg ttgatgaata
    35341 acctccacca ggcggatgga ttcactatca agccccaggg attcacggcg ctgccagaca
    35401 gcatctaccc gcgcgaataa ttcaccgttc agatagatat cattagccag ttccgccagt
    35461 tcagcggaaa actgttcatc aagacgctgt aattcatcat tggtatgcgc cgcagtcatc
    35521 gcaaaaaaga cgctggtaac gcgggtaagt aattctccgc tttgttccag tgccagaata
    35581 gtattgttga aatcaggcgt ttgcgggtta agcgcgatgg cagcaatttc tgcccgcttt
    35641 tgctgcattc cctcatcgaa tgccgggcga tagtgatgat tggcaatttg atcaaaatga
    35701 ggagccagat acggcagtgt gctttgcaca aggaaaggat tcattgttgt cattttcttc
    35761 tcctgaacgc gaggtgttcc atagcgtagg cttactgata acggagtgca atcttgcaaa
    35821 ccagtattac ccgctcttaa gcatcacgtg ctatgttatt gacacacaaa agcgttgagg
    35881 aacagtgaga tgatcgtttt agtaactgga gcaacggcag gttttggtga atgcattact
    35941 cgtcgtttta ttcaacaagg gcataaagtt atcgccactg gccgtcgcca ggagcggttg
    36001 caggagttaa aagacgaact gggagataat ctgtatatcg cccaactgga cgttcgcaac
    36061 cgcgccgcta ttgaagagat gctggcatcg cttcctgccg agtggtgcaa tattgatatc
    36121 ctggtaaata atgccggctt ggcgttgggc atggagcctg cgcataaagc cagcgttgaa
    36181 gactgggaaa cgatgattga taccaacaac aaaggcctgg tatatatgac gcgcgccgtc
    36241 ttaccgggta tggttgaacg taatcatggt catattatta acattggctc aacggcaggt
    36301 agctggccgt atgccggtgg taacgtttac ggtgcgacga aagcgtttgt tcgtcagttt
    36361 agcctgaatc tgcgtacgga tctgcatggt acggcggtgc gcgtcaccga catcgaaccg
    36421 ggtctggtgg gtggcaccga gttttccaat gtccgcttta aaggcgatga cggtaaagcg
    36481 gaaaaaacct atcaaaatac cgttgcattg acgccagaag atgtcagcga agccgtctgg
    36541 tgggtgtcaa cgctgcctgc tcacgtcaat atcaataccc tggaaatgat gccggttacc
    36601 caaagctatg ccggactgaa tgtccaccgt cagtaatttt tatacccggc gtaactgccg
    36661 ggttattgct tgtcacaaaa aagtggtaga ctcatgcagt taactcactc acaagcaaga
    36721 acgaatgacc gtcgaaacgc aacttaatcc cacacagcct gtcaatcagc agatttatcg
    36781 tattcttcgt cgcgacattg tccattgcct gattgcacca ggcacaccgt tgtcggaaaa
    36841 agaagtttct gttcgtttca atgtgtcacg ccagccggtt cgtgaagcct ttattaaact
    36901 ggcggaaaac ggcctgattc aaattcgtcc gcagcgtggc agctacgtca acaaaatttc
    36961 catggcccag gtgcgaaacg gcagttttat ccgtcaggcc attgagtgcg cggtggcgcg
    37021 tcgggcggcg agcatgatta ccgaaagcca gtgctaccaa ctggaacaaa atcttcacca
    37081 gcaacgcatt gccattgagc gcaagcaact ggatgatttt tttgaacttg atgacaactt
    37141 ccatcaactc ctgacgcaga ttgccgactg tcaactggcg tgggatacca ttgagaacct
    37201 gaaagcgacc gttgatcgcg tgcgctatat gagtttcgac catgtttctc caccagaaat
    37261 gctgttacgc cagcatcttg atattttctc tgccctgcaa aaacgtgatg gcgatgcggt
    37321 agaacgtgca atgacgcaac atttgcagga aatcagcgaa tccgtgcgcc agatccgcca
    37381 ggaaaacagc gactggttta gcgaagagta attcatttac tctcatccca tccgggatga
    37441 gaggcttttc cccccgcctt acggctcatg catgcatcaa aaaagatgtg agcttgatca
    37501 aaaacaaaaa atatttcact cgacaggagt atttatattg cgcccgttac gtgggcttcg
    37561 actgtaaatc agaaaggaga aaacacctat gacgacctac gatcgtaacc gtaacgcaat
    37621 caccactggc agccgtgtta tggttagcgg caccggtcac actggcaaga tcctgtcgat
    37681 tgatactgaa ggtctgaccg ctgagcaaat ccgccgcgga aaaaccgtag ttgttgaagg
    37741 ttgtgaagag aaactggcac ctctggacct gattcgtctc ggcatgaact aagtgtgtga
    37801 atgccgccga tggcggcatt gcttttttta cttcacggaa tatttcgcca cggtcgcttt
    37861 cgcgccatgc gctaataaag acaagtacgc ttccgtcact tttgcagtaa acaagctatt
    37921 gtctggcaaa tcatcaccaa agattgcctt aatcgccagc aatgactgga cgcgcgcttt
    37981 cccttcggca ctactttgta cagccttctg aataacaggt aacagtgggt cactgatttc
    38041 tatcggattt ccctgttcat caacaccacc gacataacgc atccaacccg cgacgcccag
    38101 cgccagcaga tcgaacttgc tgtcatgcgc cagatgccag cgaacagaat ccaacatccg
    38161 ctgtggcaat ttctggctac catccatcgc aatctgccag gttcgatgac gtaacgccgg
    38221 attgctatag cgttcaatta atcggttagc gtaatcttgc aaatcaacgc cctgcacttt
    38281 cagcgtcggc gcttgttcct gcaacatcaa gccatacgcc gcataacgat aatgttcatc
    38341 ttccatgcag tcattaatgt gctgatatcc ggcaagataa cccaaatacg ccaggaatga
    38401 atgactgccg ttgagcatgc gcaacttcat ctcttcataa ggcagcacat cactaaccag
    38461 ttcggctccc gctttttccc attctggacg tccggcaaca aagttatctt ctattaccca
    38521 ctggcggaaa ggttcacagg caacgcccgc aggatcgccc acaccggtaa gttgttcgat
    38581 tttcgccagc gtatcctctg tcactgcggg cacaatacgg tccaccattg ttgatgggaa
    38641 agtcacgtta tcttcgatcc attgtgccag ttttacatca acagctttcg cgtatgaagt
    38701 gacaacgtca cgcataacat gaccgttttc tggcatgttg tcacatgaca tgacggtaaa
    38761 tgcgggaagt cctgccgctt tacggcgggc cagcgcctca acaatcaccc ctgttgctgt
    38821 tttcggctgg tggggatttt gcacgtcggc agcgaccatc gggtgatcga gcattaactg
    38881 tccggtcgcc ggagagtgga aatacccttt ttcggtgatt gtcagagaga caatcgcgat
    38941 ttgcggttca cacatcgcag ccaacacggt ttctaagcca tccatctgta cgtgcaaggc
    39001 ttttttaacg acgccaacga cgcgagccgt ccacgcatcg gccgacattt ccgcaacggt
    39061 ataaagatta tcttgctgtt gtaaatcggc aatttgctgt tcgccgccga ttaagttgac
    39121 ctcataatat ccccagtcac tgaaatgttc tgtagcaaga atatcggcat acacgccctg
    39181 atgcgcacgg tgaaatgcac caaagcctaa atgaataatt cttggggcca ggttattacg
    39241 atcataaaca gggagtgtcg cttttgctga taacaaatta tttcccataa caattcctta
    39301 aatttaaata tggcaagcta tatgttttgt tatatgaata aaaatcccct ctccggtaag
    39361 agaagggatt gagggtttac agacttctgg aaggttgcgc agctcttaca acacgcggtt
    39421 gatcttccgc agcgtcttcc agcgcactta aatcacggtc tttcacctct ggcattttca
    39481 gcgcagagat taaaccaatt actgaatatg ccatgatcat aatggcgatc ggataccagg
    39541 attccgtcat ggtgcagaaa atacccgcca ggataggacc aaaaccggaa gcgataatac
    39601 caccaatttc tttagaaata gccatccggg taaagcggtt tttacagccg aacatttctg
    39661 ccatggtaat gttttccaga gcaaataatc ccagcaccgc acagttatga atcacaatca
    39721 gtgcaaccat aatggtgctc ggggcatagc ttttatctac aatgatagaa agcattggcc
    39781 atgccagcac aatcgcggag gtattcataa taatatacgg gatccggcga ccaattttat
    39841 cggataacca accaaggaac ggaatggtca taaagccgag aatcaaacta atcatcaatg
    39901 catctgttgg aattgctttg ttaaacaata acgtctgcac taaatagcct gcaaggaaag
    39961 tctgaattaa cccggagtta cccgcctgac caaaacgcag ccctgttgcc agccagaagg
    40021 atttgctctg gaacatgcta ccagcaggtg caggttttgc tgtgggttgg ttgctgtcat
    40081 taaccttctc aaagaccggg ctttctttca gattcatacg taaccagata gcaaagacca
    40141 tcacgacaac gctcgccagg aacggtatac gccatcccca cgccagcagt tcctctttac
    40201 tgagaatgaa gaacataaag gcccagattg ccgttgcgct caaggttccg cagttagttc
    40261 ccatagctac aaatgaggag ataattccgc gcttaccttt tggtgcatat tccgccagca
    40321 tcgtaccggc accggaaatt tccgcacctg cacccaaccc ctgaataata cgcagcgtca
    40381 ccagcaagat gggggcaaaa acaccaatct gtgcataggt cggtaacaca ccaattaagg
    40441 tggtacagat ccccatcatg gtgatggtaa taaagagcac ttttttacgc cctattctgt
    40501 cgcccatttt gccgaaaata aatgctccga caatacgcgc cacataacct gcaccgtagg
    40561 ttcccattgc caaaattaac gccattgccg ttgatgattc aggaaaaaat atttcatgaa
    40621 acactaacgc tgcgccgagc gaatataact ggaaatccat aattcacagg tgttttttcc
    40681 catcctgtgg tttccttggc gttttctagg ttttttcaga tagttgcatt ttttaaaaag
    40741 catcctaagt tcgatctcag tgtctatctg gggcctattt ctgtcccata tatgccccaa
    40801 aaaaactccc caacagataa gtagtttttt catggattta cgcgtaaaat caataacggc
    40861 tggaaatcat tcaatactcg cactatcgaa aaatttacca gccaaccgca gcacgttctt
    40921 gcataaggcg tgtctgcggt ttttcaacta ttcagataca tcactcccat cacattcatt
    40981 cctccgcatc aaaggcatat aggctatatc accttgatat ttttcttctt cagataaaaa
    41041 ctgttatcta tgtatacttt taaacccaat ccgtgtagag tctctgcata agatagtttg
    41101 cagttgccac ttcagcttgc gccataaacc gcctgatttt tgccgccacc tgttagcatt
    41161 cctgtatacc tgaaacgaca atgtttatct acgaacttta agaacaccca ggataaaaat
    41221 tgtcaactat atcatatata acacattact aattcgaggc tatatgaaca gcatactgat
    41281 aatcacttcg ctccttatca tattcagcat ttttagtcat gctctaataa aattagggat
    41341 tggcatatcc aataacccag acaaaaccga tgtataagtc aacatatcct gaatcagaca
    41401 tacaatatcg caatgaaaat caataatatt ttaaggaata tcttcatgaa atcaaaagac
    41461 accctaaagt ggttccctgc gcagcttcct gaagtaagaa ttatcctagg ggatgctgta
    41521 gtggaagtag caaaacaggg aagacctatc aataccagaa cattgcttga ttacattgaa
    41581 ggaaacataa agaaaaagtc atggctggat aacaaagaat tattacaaac agcgatatca
    41641 gttcttaaag acaacaaaaa tttaaatggt aa
//
LOCUS       DAZFQD010000028        41003 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.028, whole genome shotgun sequence.
ACCESSION   DAZFQD010000028 DAZFQD010000000
VERSION     DAZFQD010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 41003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41003
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.028"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(94..927)
                     /gene="fdhD"
                     /locus_tag="ACHKTW_001565"
     CDS             complement(94..927)
                     /gene="fdhD"
                     /locus_tag="ACHKTW_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HFO6462616.1"
                     /translation="MKKTQQKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGQLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
     gene            1121..4171
                     /gene="fdnG"
                     /locus_tag="ACHKTW_001566"
     CDS             1121..4171
                     /gene="fdnG"
                     /locus_tag="ACHKTW_001566"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:1706..1708,aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HFO6462617.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTC
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNREYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            4184..5086
                     /gene="fdoH"
                     /locus_tag="ACHKTW_001567"
     CDS             4184..5086
                     /gene="fdoH"
                     /locus_tag="ACHKTW_001567"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HFO6462618.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            5083..5718
                     /gene="fdoI"
                     /locus_tag="ACHKTW_001568"
     CDS             5083..5718
                     /gene="fdoI"
                     /locus_tag="ACHKTW_001568"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HFO6462619.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHHNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            5715..6644
                     /gene="fdhE"
                     /locus_tag="ACHKTW_001569"
     CDS             5715..6644
                     /gene="fdhE"
                     /locus_tag="ACHKTW_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HFO6462620.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQEVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(6974..7216)
                     /gene="yiiF"
                     /locus_tag="ACHKTW_001570"
     CDS             complement(6974..7216)
                     /gene="yiiF"
                     /locus_tag="ACHKTW_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YiiF"
                     /protein_id="HFO6462621.1"
                     /translation="MNSLVGIDMGRILLDLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLINQSQTARTSVPGIWQGCEEDGVEYQRKLREEW"
     gene            complement(7436..7654)
                     /gene="yiiE"
                     /locus_tag="ACHKTW_001571"
     CDS             complement(7436..7654)
                     /gene="yiiE"
                     /locus_tag="ACHKTW_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032993 - protein-DNA complex [Evidence
                     IEA];
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HFO6462622.1"
                     /translation="MAMNTVFLHLSEEAIKRLNKLRGWRKVSRSAILREAVEQYLERQ
                     QFPVRKAKRGRQRGETVGVYDQCKDHKE"
     gene            complement(8240..8530)
                     /gene="nadS"
                     /locus_tag="ACHKTW_001572"
     CDS             complement(8240..8530)
                     /gene="nadS"
                     /locus_tag="ACHKTW_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
                     /protein_id="HFO6462623.1"
                     /translation="MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRAS
                     GLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALLRAIANDPQHVLQALNR"
     gene            complement(8531..8842)
                     /locus_tag="ACHKTW_001573"
     CDS             complement(8531..8842)
                     /locus_tag="ACHKTW_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO6462624.1"
                     /translation="MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGG
                     LRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIYRKGIKDDLSPQEKAMLRLLNTR
                     W"
     gene            9071..9979
                     /locus_tag="ACHKTW_001574"
     CDS             9071..9979
                     /locus_tag="ACHKTW_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO6462625.1"
                     /translation="MALEKGIAELVEKFIAAGRPSSRQQSIAQRREGYIASAVLAGET
                     ETRVDIQTIELEGMTLRIVSPLNAPTLLPTIIYYYGGCFVSGGFATHDNQLRQLAYYG
                     QCRVIAVQYRLAPEHTFPAAHDDAQRGAEIVWQHAERLGVDKQRITLAGDSAGGHLAL
                     VTALRLKRAGEWQPAQLILIYPMLDATAHFESYIRNGHDYIITRDTLLSGFEMYLPGI
                     ERRHPEASPLWRDDFNGLPPVHIITAEYDPLCDEGEALYHRMTGQGVQCTCQRYLGVI
                     HGFFQLGGISEAARSALRDVAWRAGR"
     gene            complement(10043..10984)
                     /gene="fabY"
                     /locus_tag="ACHKTW_001575"
                     /gene_synonym="yiiD"
     CDS             complement(10043..10984)
                     /gene="fabY"
                     /locus_tag="ACHKTW_001575"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HFO6462626.1"
                     /translation="MYHLRVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMAVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFVNQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
     gene            complement(11029..11466)
                     /gene="dtd"
                     /locus_tag="ACHKTW_001576"
     CDS             complement(11029..11466)
                     /gene="dtd"
                     /locus_tag="ACHKTW_001576"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFO6462627.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            complement(11463..12335)
                     /gene="yihY"
                     /locus_tag="ACHKTW_001577"
     CDS             complement(11463..12335)
                     /gene="yihY"
                     /locus_tag="ACHKTW_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HFO6462628.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            complement(12329..12928)
                     /gene="yihX"
                     /locus_tag="ACHKTW_001578"
     CDS             complement(12329..12928)
                     /gene="yihX"
                     /locus_tag="ACHKTW_001578"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HFO6462629.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRIPLASLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            complement(13069..13956)
                     /locus_tag="ACHKTW_001579"
     CDS             complement(13069..13956)
                     /locus_tag="ACHKTW_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HFO6462630.1"
                     /translation="MKTERKNEHYLALQQAFDAPWPGPVGELVTLEKGNIHLQIYPHD
                     GARITSLKAFGSEVLRQWQPQRRAFQYGCFPMVPWAGRLGNATLNAGGQCYSLPANKP
                     PHALHGMACYSTWEIIDKTADSLTLRMPLASPWPWQGEVIQTFLLENDALVLQLEVHS
                     YADTFPASAGWHPWFAKKLTPQNTESLQVLFDADWQEEAGSDELPTGNRISPQAGPWD
                     DCFGFYDGVKVKLLWPGKLAMTMISSANSLVVFDKQPDATCINPLTQAPNAINLTPEL
                     VTPDRPLVIETRWQFTPES"
     gene            complement(13946..15295)
                     /locus_tag="ACHKTW_001580"
     CDS             complement(13946..15295)
                     /locus_tag="ACHKTW_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO6462631.1"
                     /translation="MSEKLPAPREGLSGKAMRRVVMGSFAGALMEWYDFFIFGTAAGL
                     VFAPLFYPDSDPFIGLIAAFATFGVGFLTRPLGGIVFGHFGDKIGRKITLIWTLAIVG
                     CSTFLIGFIPTYQEIGIWAPLILMALRLIQGFGLGGEYGGAALMTIESAPESRRGFLG
                     SLPQTAASVGIMLATGIFALCNHFLTSEQFLSWGWRIPFWLSAVMLIVGLFIRLHTEE
                     TLDFQKQKTTNNKEKSVPPLIELFKKHPRNILLALGARLAESVSSNIINAFGIVYISS
                     QLALSRDIPLTGMLIASAIGIFSCPLVGWLSDRIGQKSLYLSGAGFCVLFAFPFFLLL
                     DSKSTLIIWCSMILGYNLGPTMMFAVQPTLFTRMFGTKVRYTGLSFAYQFSAILGGLS
                     PLIASSLLALGGGKPWYVALFLFAVSVLSFVCVWLIEPTDEQETASYRYIREQSHEN"
     gene            complement(15394..16827)
                     /locus_tag="ACHKTW_001581"
     CDS             complement(15394..16827)
                     /locus_tag="ACHKTW_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO6462632.1"
                     /translation="MSQITSPATYSISRPQDVIDIVNKNSAINTSIGVIFIALGGILI
                     DAYQAAMVGFGNKYIAAQFGISPGLAATVNASVLIAALIGGLLANRVINRFGQKRAFI
                     IGMGLCTIGAAAVAIAPSIWWVLVCRVIMGFGLGIDFPLATNAVAELRGSTSKKTGTS
                     VNLWQMAWYVSTTVVYLVLLPLLLSGIAEEQLWRYGIFIGAIFAVIFMILRYFFIGES
                     AMWAARVGRYQEACDILGKRYGVQARVAASSTTEAKFSEKAENKYSGGYGILFNDRYR
                     KRTILGCVVATMQAWQYNAVGVYLPLTLAGIISGGLTGALTGSAVVNALCGVTGGMIG
                     SFILQRLGTRRQSMYGFAVVTLALLSLGALATTNPWLSLGLLGSIIFFHSAGPGGLGM
                     TIATLSYPPAIRPTGVGFARAIMRTGAIAGLIFWPMLWGALKTEAFYWLAIVPFLGFL
                     TCVLINWEPLGANVDAEDAEVLAELKK"
     gene            complement(16897..17907)
                     /locus_tag="ACHKTW_001582"
     CDS             complement(16897..17907)
                     /locus_tag="ACHKTW_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001494590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HFO6462633.1"
                     /translation="MDKITQVLFSDIGKVTTQYVEVPHQELKPHEVRIAPVFYGICGS
                     DLHVLKGGHPFAKPPVVPGHEIAARVTEVGSDVKNVQPGDHVVVDPIMACMECRACKA
                     GRFNLCEPPQVAGFRAPGFARSQHIVPARNCHVAPASLPLKVLAFAEPAACARHCVNR
                     MPKASLESVLVIGAGTIGLSIVQALRIMGAGKITVIEPDAAKRALALKLGAAEVWAPG
                     ELAADVRFTGAIDVVAAQATLNDACTRVYAGGTVVCMGVPSGPREIPLPMMQRFERDL
                     LNSGMYIPEDFDAVIEWLADGRFDTSELVTDLFAIEDAAAAFERAQQNDSIKVMLQFA
                     PE"
     gene            complement(17940..18827)
                     /locus_tag="ACHKTW_001583"
     CDS             complement(17940..18827)
                     /locus_tag="ACHKTW_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HFO6462634.1"
                     /translation="MSSHFTPFTLKDISRPGGGFAMLAVDQREAMRLMFAAAGQPKPI
                     ADSVLTDFKVAATRILSPYASAVLADKQFCLEQIVEQGAVANSCGLIVAADLFIPGNG
                     IPVDSVEIDMSVDPHKAREMGAKAMKLLVLWREDEPAEERLAMVDKFVRRCRSAGLVS
                     IIEPVVRPPRRGWDFDRESAIVAAAAELGGTEADLYKAEMPLGGKGDEKTLLAACQQL
                     NDQMKMPWVILSSGVDADIFGRAVSIAMKGGASGFLAGRAVWASVVGAQDPQTMLRDV
                     SVPRLQRLAEIVDEGIAQR"
     gene            complement(18852..19730)
                     /gene="yihU"
                     /locus_tag="ACHKTW_001584"
     CDS             complement(18852..19730)
                     /gene="yihU"
                     /locus_tag="ACHKTW_001584"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001318159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HFO6462635.1"
                     /translation="MATIAFLGLGQMGSPMANNLLQKGHSLQVFDVNAQAVDALVTQG
                     ATAAQTPAEAAAGAEFIITMLPNGDIVRQVLLGEKGVCEAVSSDALLIDMSTIHPLQT
                     DALIRELQEKGISMMDAPVGRTSVNAIDGTLLILAGGTDEQIARARPILMCMGNELVE
                     AGGPGMGIRVKLINNYMSIALNALSSEAAVLCESLGLNLDVAIKVMSGTAAGKGHFTT
                     TWPCKVLKGDLSPAFMVDLALKDLRIAVDVARETGAPLNMGIAAESYYAAASQDGKGR
                     QDWSALLNQVRQQAGL"
     gene            19903..20799
                     /locus_tag="ACHKTW_001585"
     CDS             19903..20799
                     /locus_tag="ACHKTW_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFO6462636.1"
                     /translation="MTKIACVGITVQDRIYSLLTLPDGGGKYQANHYLEIGGGPAATA
                     AVAIAKLGVEVDFIGRVGDDSCGNTLLAELEGWGVNTAFCRRYPNARSSQSAILVDQH
                     GERIIVNYPSPDLGTDAEWLEAIDFSRYDLILADVRWHSGTEKAFSLARLAGVTTLLD
                     ADMTPQDISPLVALADHAVFSTPGLKRMTGLQSPEEGLFQATTQTAGKVYVTLGSEGS
                     LWIEDGHLCQQEAFSVNVVDTTGAGDVFHGALAVALAEKMPTKEAIRFASAVAAMKCT
                     QPGGRAGIPNREQTESFLSLYA"
     gene            20839..21627
                     /locus_tag="ACHKTW_001586"
     CDS             20839..21627
                     /locus_tag="ACHKTW_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFO6462637.1"
                     /translation="MSIIEVTGNPRHDQLVHLIAERGYMNIEELAQLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRVSSVMNTAFEQRELSLTAEKRAIAEAVADYLPERCTVFI
                     TIGTTVEAVARALLNRRDLRIITNSLRVAQILYKNQDIEVMVPGGTLRAHNGGIIGPG
                     AVDFIEGFRADYLITSIGAIEHDGTLLEFDVNEALVARTMIKHARNTLLVADHTKFAA
                     SAAVSIGNARNVRAFFTDAPPPNSFCQLLSEENVELVVAEQEVS"
     gene            complement(21738..23003)
                     /gene="yihN"
                     /locus_tag="ACHKTW_001587"
     CDS             complement(21738..23003)
                     /gene="yihN"
                     /locus_tag="ACHKTW_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO6462638.1"
                     /translation="MLTKKKWALFSLLTLCGGTIYKLPSLKDAFYIPMQEYFHLTNGQ
                     IGNAMSVNSFVTTVGFFLSIYFADKLPRRYTMSFSLIATGLLGVYLTTMPGYWGILFV
                     WALFGVTCDMMNWPVLLKSVSRLGNSEQQGRLFGFFETGRGIVDTVVAFSALAVFTWF
                     GSGLLGFKAGIWFYSLIVIAVGIIIFFVLNDKEEAPSVEVKKEDGASQNTSMTSVLRD
                     KTIWLIAFNVFFVYAVYCGLTFFIPFLKNIYLLPVALVGAYGIINQYCLKMIGGPIGG
                     MISDKILKSPSKYLCYTFIISTAALVLLIMLPHESMPVYLGMACTLGFGAIVFTQRAV
                     FFAPIGEAKIAENKTGAAMALGSFIGYAPAMFCFSLYGYILDLNPGIIGYKIVFGIMA
                     CFAFCGAVVSVMLVKRISQRKKEMLAAEA"
     gene            complement(23105..24085)
                     /gene="yihM"
                     /locus_tag="ACHKTW_001588"
     CDS             complement(23105..24085)
                     /gene="yihM"
                     /locus_tag="ACHKTW_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HFO6462639.1"
                     /translation="MVTINNARKILQRVDTLPLYLHAYAFHLNMRLERVLPADLLDIA
                     SENNLRGVKIHVLDGERFSLGNMDDKELSAFGDKARRLNLDIHIETSASDKASIDEAV
                     AIALKTGASSVRFYPRYEGNLRDVLSIIANDIAYVRETYQDSGLTFTIEQHEDLKSHE
                     LVSLVKESEMESLSLLFDFANMINANEHPIDALKTMAPHITQVHIKDALIVKEPGGLG
                     HKACISGQGDMPFKALLTHLICLGDDEPQVTAYGLEEEVDYYAPAFRFEDEDDNPWIP
                     YRQMSETPLPENHLLDARLRKEKEDAINQINHVRNVLQQIKQEANHLLNH"
     gene            complement(24093..24803)
                     /gene="yihL"
                     /locus_tag="ACHKTW_001589"
     CDS             complement(24093..24803)
                     /gene="yihL"
                     /locus_tag="ACHKTW_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFO6462640.1"
                     /translation="MTGNQSTVENAKEKLDRWLKDGITTPGGKLPSERELGELLGIKR
                     MTLRQALLNLEAESKIFRKDRKGWFVTQPRFNYSPELSASFQRAAIEQGREPSWGFTE
                     KNRTSDIPETLAPLIAVTPSTELYRITGWGALEGHKVFYHETYINPEVAPGFIEQLEN
                     HSFSAVWEKCYQKETVVKKLIFKPVRMPGDISKYLGGSAGMPAILIEKHRADQQGNIV
                     QIDIEYWRFEAVDLIINL"
     gene            complement(25020..26843)
                     /gene="typA"
                     /locus_tag="ACHKTW_001590"
     CDS             complement(25020..26843)
                     /gene="typA"
                     /locus_tag="ACHKTW_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HFO6462641.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            27216..28625
                     /gene="glnA"
                     /locus_tag="ACHKTW_001591"
     CDS             27216..28625
                     /gene="glnA"
                     /locus_tag="ACHKTW_001591"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HFO6462642.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            28798..29847
                     /gene="glnL"
                     /locus_tag="ACHKTW_001592"
     CDS             28798..29847
                     /gene="glnL"
                     /locus_tag="ACHKTW_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HFO6462643.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            29859..31268
                     /gene="glnG"
                     /locus_tag="ACHKTW_001593"
     CDS             29859..31268
                     /gene="glnG"
                     /locus_tag="ACHKTW_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HFO6462644.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNVQLNGPTTDIIGEAPAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRAKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            31380..31490
                     /gene="yshB"
                     /locus_tag="ACHKTW_001594"
     CDS             31380..31490
                     /gene="yshB"
                     /locus_tag="ACHKTW_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HFO6462645.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(31680..33053)
                     /gene="hemN"
                     /locus_tag="ACHKTW_001595"
     CDS             complement(31680..33053)
                     /gene="hemN"
                     /locus_tag="ACHKTW_001595"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HFO6462646.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFQFNADAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVDEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPIEKQW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(33242..33751)
                     /gene="yihI"
                     /locus_tag="ACHKTW_001596"
     CDS             complement(33242..33751)
                     /gene="yihI"
                     /locus_tag="ACHKTW_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HFO6462647.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELL
                     ETDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            complement(33870..34036)
                     /locus_tag="ACHKTW_001597"
                     /pseudo
     CDS             complement(33870..34036)
                     /locus_tag="ACHKTW_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546704.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            34333..34965
                     /gene="yihA"
                     /locus_tag="ACHKTW_001598"
     CDS             34333..34965
                     /gene="yihA"
                     /locus_tag="ACHKTW_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFO6462648.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKAQLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            complement(35347..38133)
                     /gene="polA"
                     /locus_tag="ACHKTW_001599"
     CDS             complement(35347..38133)
                     /gene="polA"
                     /locus_tag="ACHKTW_001599"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFO6462649.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKPAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISRERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKEPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKEQGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            38497..39429
                     /gene="yihG"
                     /locus_tag="ACHKTW_001600"
     CDS             38497..39429
                     /gene="yihG"
                     /locus_tag="ACHKTW_001600"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HFO6462650.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWSLDMPFMKRYSRAYLLRH
                     PERRGKDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQNK"
     gene            complement(39470..40900)
                     /gene="yihF"
                     /locus_tag="ACHKTW_001601"
     CDS             complement(39470..40900)
                     /gene="yihF"
                     /locus_tag="ACHKTW_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HFO6462651.1"
                     /translation="MIRKSATGVIVALAVIWGGGTWYTGTQIQPGVEKFIKDFNDAKK
                     KGEHAYDMTLSYQNFDKGFFNSRFQMQMTFDNGAPDLNIKPGQKVVFDVDVEHGPLPI
                     TMLMHGNVIPALAAAKVNLVNNELTQPLFIAAKNKSPVEATLRFAFGGSFSTTLDVAP
                     AEYGKFSFGEGQFTFNGDGSSLSNLDIEGKVEDIVLQLSPMNKVTAKSFTIDSLARLE
                     EKKFPVGESESKFNQINIINHGEDVAQIDAFVAKTMLDRVKDKDYINVNLTYELDKLT
                     KGNQQLGSGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAVHPELANDEVALQEVNAAL
                     FKEYLPLLQKSEPTIKQPVRWKNALGELNANLDISIADPAKSSSSTNKDIKSLNFDVK
                     LPLNVVTETAKQLNLSEGMDAEKAQKQADKQISGMMTLGQMFQLITIDNNTASLQLRY
                     TPGKVVFNGQEMSEEEFMSRAGRFVH"
BASE COUNT        10289 a        10575 c         9931 g        10208 t
ORIGIN      
        1 gccactacac gcgttttatt tataaataaa ataataaaat tcacgaaatg aataagtcct
       61 gcaatttaat atatatcgca ggacttattt taattaattg ctcaaacgct gcggatgggt
      121 ataaaccgtt gcccgacccg gtttacaaaa acctaccagt gtcagattac agcgctcagc
      181 cacttctaca gcaagcgtgg tcgcggcaga caccgcaaac aaaatctcta cgccgcacat
      241 cgccgacttt tgcaccattt cataactggc acgactggaa accagtaccg caccttgctg
      301 ccagctttcc ccttcttgtg accggcggcc taacagtttg tccagcgcca catggcgacc
      361 cacgtcttca tgcccgccga ccagttcgcc agatggcaac atccaggcag cggcgtgagt
      421 acaaccagtc agttgcccca ctggctgaaa atcgttgaga tgacgtaatg catcatccag
      481 tttgttgaga tcaaacgtct gggtgaacgg tagcggctgc accggttttc cgatgtcatt
      541 aagttgctcc acgccgcata cgccacagcc cgtacgtccc gccagcgccc ggcggcgctc
      601 cttcaacccc ataaagcggc ggctggaaag ctcaatttgt acttcaagac cattacagga
      661 aggaacgaca tccatgccga agatatcgcg cggactttct attatcccct cggaaagcga
      721 aaaaccgagc gcaaagtact caaggtcttt gggcgacgcc atcatcacca catgcgaaat
      781 gccgttgtag accagcgcaa cgggaacttc ttccgcgacc tcatctaacc gtgggtgttg
      841 taaatcatca cgccgccata actcaatttg acgaacacct gtgatatttg tcacattttc
      901 gatttctttt tgctgtgttt ttttcacttt ctcttaacca gataagaaca cacacaccaa
      961 cattatggta ttctgttaca aacccttctt ggatggaggg aaattgagcc aattctggac
     1021 ctttgcggcc ccttccgcaa agaaaaataa ctcccactcc ctgcacatgc agcaagcgaa
     1081 tgtaaatggg acgtgacaat gtcgaaacaa ggagcaatcc atgcaggtca gcagaaggca
     1141 gttctttaag atctgcgctg gcggtatggc aggcaccacg gcagcggcac tgggttttgc
     1201 acccagcgta gcactcgcgg aaacccggca gtataaactg ctgcgcacct gcgaaacccg
     1261 taatacctgc acctattgtt ccgtaggctg tgggctgttg atgtacagcc tcggtgacgg
     1321 agcaaaaaac gccaaagcat ctatcttcca tatcgaaggc gatccggatc acccggtcaa
     1381 ccgcggtgca ctttgtccga aaggcgctgg cctggtggat ttcatccact ccgaaagccg
     1441 tctgaagttt ccggaatacc gtgcgccagg ttctgataaa tggcagcaaa tcagttggga
     1501 agaggcgttt gatcgcatcg ccaaactgat gaaagaagac cgcgatgcta actacattgc
     1561 gcaaaacgcc gaaggcgtga ctgttaaccg ctggctctcc accgggatgc tgtgtgcttc
     1621 cgcgtcgagt aacgaaaccg gctatttaac gcaaaaattc tcccgcgcgc tgggtatgct
     1681 cgcggtcgac aaccaggcgc gtgtctgaca cggaccaacg gtagcaagtc ttgctccaac
     1741 atttggtcgc ggtgcgatga ccaaccactg ggtcgacatc aagaacgcca acctcgtcgt
     1801 ggtgatgggc ggtaacgccg ctgaagctca cccggtcggg ttccgctggg cgatggaagc
     1861 caaaattcac aacggcgcga agctgattgt gatcgatcct cgctttacgc gtacggcggc
     1921 ggtagctgac tactatgccc ctattcgttc cggtactgac attgctttcc tgtcaggcgt
     1981 attgctgtac ctgctgaaca atgaaaaatt caaccgcgaa tacaccgaag cctataccaa
     2041 cgccagcctg atagtccgtg aggattacgg ctttgaagat ggcctgttca ccggctacga
     2101 cgcggaaaaa cgcaagtacg ataaatcctc ctggacttat gaactggacg aaaacggctt
     2161 cgccaaacgc gataccacgc tgcaacatcc gcgctgcgtg tggaacttgc tgaaacagca
     2221 cgtttcccgc tacacgccag atgtggttga aaacatctgt ggtacgccaa aagacgcgtt
     2281 cctgaaagtc tgcgaataca tcgcagaaac cagtgctcac gataaaactg cctcgttcct
     2341 gtatgccctc ggctggacgc aacactccgt tggtgcgcaa aacattcgta cgatggcgat
     2401 gatccagctg ctgctcggca atatggggat ggccggcggc ggcgttaacg ccctgcgcgg
     2461 tcactccaat attcaggggc tgacggacct ggggctactg tcgcagagtc tgccaggtta
     2521 catgacgctg ccaagcgaga agcagaccga tctgcaaacc taccttaccg ccaacacgcc
     2581 aaaaccgctg ctggaaggcc aggtaaacta ctggggcaac tacccgaaat tcttcgtctc
     2641 tatgatgaag gccttctttg gtgataaagc gacggcggaa aatagctggg gctttgactg
     2701 gttgccgaag tgggataaag gctacgacgt cctgcaatac ttcgagatga tgaaagaagg
     2761 caaggtcaat ggctatatct gccagggctt taaccctgtt gcctcattcc cgaacaaaaa
     2821 caaagtgatc ggttgtctgt cgaaactgaa gttcctcgtc accatcgacc cgctgaacac
     2881 tgaaacctct aacttctggc agaaccacgg tgagctgaac gaagttgact cgtcgaagat
     2941 ccagaccgaa gtgttccgtc tgccatcgac ctgcttcgca gaagagaacg gttcaatcgt
     3001 taactcaggt cgctggttgc agtggcactg gaaaggtgcg gacgccccgg ggattgcgct
     3061 gactgacggt gagatcctct ccggtatctt cctgcgcttg cgcaagatgt atgccgaaca
     3121 gggtggcgcg aacccggatc aggtgctgaa catgacctgg aactacgcca ttccgcatga
     3181 gccgaagtcg gaagaagtag cgatggagag caacggtaag gcgctggccg atattaccga
     3241 tccggcaacc ggtgcggtta tcgtcaagaa aggccaacaa cttagctcgt tcgcccaatt
     3301 gcgcgatgac ggtacaacct cctgtggctg ctggattttc gccggtagct ggacgccgga
     3361 aggcaaccag atggcgcgtc gtgataacgc cgatccgtct ggcctcggta acacgctggg
     3421 ctgggcatgg gcatggccgc ttaaccgccg cattctgtat aaccgcgcct ccgcagatcc
     3481 gcagggtaac ccgtgggatc cgaagcgtca gttgctgaaa tgggacggca ctaagtggac
     3541 cggctgggat attccggact acagcgcagc gcctccgggt agcggcgtcg ggccgtttat
     3601 catgcagcag gaaggcatgg ggcgtctgtt tgccctcgat aagatggcgg aaggtccgtt
     3661 cccggaacac tacgagccgt ttgaaacgcc gctgggaact aacccgctgc atccaaacgt
     3721 tatctcgaac ccggctgcac gtatctttaa agacgacgcc gaagcattgg gtaaagccga
     3781 taagttcccg tatgtcggaa ccacctatcg tctgaccgag catttccact actggaccaa
     3841 acacgcgctg ttgaacgcga ttttgcaacc agagcagttt gtggaaatcg gggagtcgct
     3901 ggcgaataaa cttggcattg cccagggcga taccgtgaaa gtctcctcca accgtggcta
     3961 tatcaaggcc aaagcggtgg tgaccaaacg tattcgtaca ctgaaagcga acggcaaaga
     4021 tatcgatacc atcggtattc cgattcactg gggctatgaa ggcgtagcta aaaaaggctt
     4081 tattgccaat acgttaacgc cattcgttgg tgatgcgaac acgcagacgc cggagtttaa
     4141 gtccttcctt gtgaatgtgg aaaaggtgta acggagacga catatggctt atcaatctca
     4201 agatatcatt cgtcgttccg cgactaacgg tctgaccccc gcgcctcagg cgcgggactt
     4261 ccaggaagaa gtggcgaaac tcatcgacgt caccacctgt atcggctgta aagcctgtca
     4321 ggtggcgtgt tcagagtgga acgacattcg cgataccgtc ggcaataaca ttggggtgta
     4381 cgacaacccc aatgatttaa gcgccaaatc gtggacggta atgcgcttct cggaagtgga
     4441 gcagaacgac aaactggaat ggctgatccg caaagacggc tgtatgcact gttccgatcc
     4501 aggctgcctg aaagcgtgcc cggcggaagg tgcaattatt cagtatgcca acggtatcgt
     4561 cgacttccag tccgagcagt gcattggctg cggttattgc attgcgggct gtccgttcga
     4621 cattccgcgc ctcaacccgg aagacaaccg cgtctacaaa tgtacgctgt gcgttgaccg
     4681 cgtggtggtt gggcaagaac cagcctgcgt gaagacctgc ccaacgggcg cgattcactt
     4741 cggtacgaaa gagtcgatga aaacgctggc gagcgagcgc gttgctgagc tgaaaacccg
     4801 cggttacgac aatgcgggtc tgtacgatcc ggcaggcgtc ggtggtacac acgtcatgta
     4861 cgtgctgcac catgctgaca agccaaatct gtatcatggc ttgccggaga acccggaaat
     4921 cagcgaaacc gtgaaattct ggaaaggcat ctggaaaccg ctcgcggctg ttggctttgc
     4981 ggctaccttt gcggccagta tcttccacta cgtgggtgtc ggtccgaacc gtgcggatga
     5041 ggaagagaat aatctgcacg aagagaaaga cgaggagcgc aaatgaaacg acgtgacacc
     5101 atcgtgcgct acaccgcgcc ggaacgtatc aaccactgga tcaccgcctt ctgcttcatc
     5161 ctggcggcgg tgagcgggct gggctttttg ttcccgtcct tcaactggtt gatgcaaatc
     5221 atgggcacac cgcagctggc gcgaattctg cacccgtttg tcggcgtggt tatgtttgcc
     5281 tcgttcatca tcatgttttt ccgttactgg catcacaacc taatcaatcg ggatgatatc
     5341 ttttgggcga agaatattcg taagatcgtc gtcaacgagg aagtaggtga caccgggcgt
     5401 tataacttcg gtcagaaatg cgttttctgg gcggcgatta ttttcctggt tctgctgctg
     5461 gtgagcggcg tgattatctg gcgtccttat tttgcgcctg ctttctcaat cccggtgatc
     5521 cgattcgcgt taatgctgca ttcatttgcc gcagtggcgt taattgtggt tatcatggtg
     5581 catatctacg ccgccctttg ggtgaaaggc acgattaccg ccatggtgga aggatgggtt
     5641 accagcgcct gggcgaagaa acatcacccg cgctggtacc gtgaggtccg caagacaacg
     5701 gaaaagaaag ctgaatgagt attcgcataa tcccgcaaga tgagctgggt tcgagcgaga
     5761 aacgtacggc ggatatgatt ccgccgttat tgttccctcg gctcaagaat ttatacaacc
     5821 gccgcgccga gcgtctgcgc gagctggcag aaaataatcc gctgggtgat tacctgcgct
     5881 ttgctgcgct tatcgcccac gcccaggaag tggtgctgta cgaccatccg ctggagatgg
     5941 atctgaccgc gcgcattaaa gaagccagcg cacagggcaa gcccccgctg gatattcacg
     6001 ttctgccgcg tgataagcac tggcaaaagc tgctgatggc gctgattgct gagctgaaac
     6061 ctgaaatgag cggcccggcg ctggcagtga ttgagaatct ggagaaggca tcaactcagg
     6121 agctggaaga tatggccagc gcactgtttg cctctgattt ctcatccgtc agcagcgata
     6181 aagcgccgtt tatctgggct gcactgtcgc tctactgggc gcagatggcc aatctgatcc
     6241 ccggcaaagc ccgcgctgaa tacggcgagc aacgtcaata ttgcccggtt tgtggttcta
     6301 tgccggtgtc cagcatggtg caaattggca ccactcaggg tctgcgttac ctgcactgca
     6361 acctgtgtga aaccgaatgg cacgtagtgc gcgtaaaatg cagcaactgc gagcagagcg
     6421 gcaaactgca ttactggtcg ctggatgacg aacaggccgc gattaaagcc gaaagctgcg
     6481 atgactgtgg cacttacctg aagattcttt atcaggaaaa agaaccgaaa gttgaagccg
     6541 tggcagatga cctcgcttct ctggtactgg acgcgcgaat ggagcaagaa ggctatgccc
     6601 gcagttccat caacccgttc ctgtttccgg gtgaagggga gtaatctctg acgttttccg
     6661 ggtggcgctg gtcgcccgga ttacagctca tagggtgtga cgacaccatc tctcgtattc
     6721 cgcgttaccc attcaagatc atgccgcaat tgtctgtttg cctcgttctt ccctccaaca
     6781 catcgataag gttatgggta tgtcactgat gacaagcgta tgattgcctg atgcgctgcg
     6841 cttatcagcc ctaccaataa acttcaatat attgaatttg catgactttg taggccggat
     6901 aaggcgttca cgccgcatcc ggcatgaaca acgcgcacgt tgtcagcaat ctgattgaca
     6961 tcattttttc ccattaccat tcctcgcgca gcttacgctg atattcgaca ccatcttcct
     7021 cacacccttg ccagatgcca ggaacactgg ttcttgctgt ttgcgattga tttatcaggt
     7081 attgatctac cgcttccctt aataggtccg cacgaggaag attacgctgc acctcgagat
     7141 catcaagttg cttaatcacc tcattcgata aatcgagtaa aattctgccc atatctatac
     7201 ctaccagaga gttcataaat atctcgccaa tatcattttt gaatctatgg agagaaaagt
     7261 acccttgtcg aatctttaaa gaaagctcat ttacgaatca ttttttattt ttgcgaggcg
     7321 ttttccagat atgatgtaac agttgcagtt attataagtt caagtaaaac aaccttgtat
     7381 taataccaga ataagggcat ctggtttttt atattcaaga ataaaaaaat ttttgtcatt
     7441 ccttatgatc cttacactga tcataaacac ctactgtctc acccctttgc ctgcctctct
     7501 ttgctttacg caccggaaat tgctgccgct ccagatattg ctcaaccgct tcgcgtaaaa
     7561 ttgcagatcg tgaaacctta cgccagcccc gaagcttgtt cagccgttta attgcctctt
     7621 ctgataaatg aagaaaaacc gtattcatcg ccattaccac caccttacat atattactca
     7681 ttaatgtatg tgcgaaaatt acgccggaaa atcatttaaa aaaagcggat ttttgcatgt
     7741 tgttttgttt ttgcgaggtg ttttccagaa ataataagag tgttgcagtt gtttcattta
     7801 ttactcaaaa atggtttagt tatcgcccag gatgagttat ggatgctatg aatatattat
     7861 attcagagat atttatttgc ataatctgaa tggtgtagcc gaaaaacgtc agcatcctaa
     7921 cagcaccagg atttaaggtg aaattaatca tccatcacag accggtcagt actgccacca
     7981 accggtcttt acttgttcat cgttataatt ttgttggtaa cagttcggga aacaaggaat
     8041 tccttccgga accccccggt atcatccgat agcattgacg tcaatcatca gcgcgacgat
     8101 gagttttttt aatcgcatca tagggtggcg acgttcagca cgattacggg tcatagcaaa
     8161 actcctgggc aggcgaccac tatgccgctt tcacaggagt tctgaatgct agtgctttct
     8221 gtatggcata accatgcctc taacggttaa gtgcttgcag cacatgttgc ggatcattgg
     8281 caatcgctcg cagcaaagcc tttgcaggcc cggtcggtga acgtcgcccc tgctcccagt
     8341 tacgcaacgt gtctacgctt acagaaataa gatcggcaaa tttagattgt gacaaccctg
     8401 aagcgcggcg tatttctttg attttcatag catcaacttc aaaagtccgc gaaggctgtc
     8461 gcgttccttc aataatttca ttcatctggg ccatgctgtg agtcaaccgc tcaaaaagtg
     8521 ccttatccat ttaccacctc gtatttaaca accggagcat tgctttttcc tggggagaca
     8581 gatcatcttt aataccctta cgataaataa gcagtaatct gatctcataa tgtttaacct
     8641 gatgaaagta aataaccctg acaccagcac gtttcccctt tcctcctgaa acccatcgaa
     8701 cctttcttaa cccaccagtt tcaggtatta cctcaccata gtcaggattc aaggccagga
     8761 aaaactgaaa acgactgaat tcatcgtcgg tcagcagttt ttgaacatct tcagtaaaaa
     8821 tttccgtttc gatgaagagc ataattatac gccattggct tagagtaaca atcctttttg
     8881 tacgtcattg acgtaccctt gaaaccacta gcttactgat agtgctccag caaaaccacg
     8941 tctctgccgg gtttgtcttt agatttgcga ggcgctttcc agatttttca cgcccgtgaa
     9001 tttccctgta tccatccagc aaaaacgggt gtataagact gtacattcat acagcaaaat
     9061 aagttctact atggcactgg aaaaaggaat tgcagagtta gtcgaaaagt ttatcgctgc
     9121 gggtcgcccc tcatcgcgtc aacaaagtat tgcgcagcgc agggagggct atatcgccag
     9181 cgctgtcctt gcgggtgaga cagaaacgcg ggtagatatt cagactatcg aacttgaagg
     9241 gatgacctta cgaatagtgt cgccgctgaa tgccccgaca ttactcccga cgataattta
     9301 ttactacggt ggctgttttg ttagcggcgg gttcgcaact cacgacaacc agctgcgcca
     9361 gttagcctat tacgggcaat gtcgggttat tgccgtccag tatcggctgg caccggaaca
     9421 tactttcccc gccgcccatg atgatgcgca acgaggtgca gaaatagtct ggcaacacgc
     9481 cgaacgactc ggcgttgata aacaacgtat aactcttgcc ggtgacagtg cgggcggaca
     9541 tctggcgctg gtaactgctt tgcgattaaa gcgtgctggc gagtggcaac cggcacaact
     9601 catcttaatt tatcctatgc ttgatgcgac tgcgcatttc gaaagctaca tacgcaatgg
     9661 tcacgattac atcatcacac gcgacacttt actgagcggc tttgagatgt atctgccagg
     9721 cattgaacgc aggcatcctg aagccagccc tctttggcgc gatgatttca atggcttgcc
     9781 acctgtacat atcattaccg ctgaatacga cccactttgc gatgaaggtg aagcgttgta
     9841 tcaccgcatg acagggcaag gagtgcaatg cacctgccaa cgctaccttg gcgttatcca
     9901 cggatttttt cagttaggtg gtatcagcga ggccgcgcga tcggcactac gggatgttgc
     9961 ctggagagcg ggaaggtaac ttcggacctg ggccctgaaa aggttttcag attgaaaacc
    10021 ttttcagtca agtaaaacgc ccctactctt cttcgttccc gccctcttca tacgggccaa
    10081 atggcttcgc gggcagaacg atatacgtgc cttcaaacac tgcacccggc gtctcgtcgc
    10141 caaagatttc aacttgcatc tgcacccgtg cttttcgtcc gcgcgccaga cggtcgagat
    10201 cgccgcttaa ggcaccaagg tcggctaccg catgaggttt accgctaatc ggcttgctgt
    10261 agcggatatg cgcatccgcc agaataatcg ttccgccgag atggcgttcg cgcagcatca
    10321 gccagataag cccccaaccg gtgagcgtcg ccagcgagaa taaactcccg gcaaacagcg
    10381 tatggtgcgg attctgattg ccggtttccg gcatggtggt gataaatttt tgcccggtat
    10441 attgttgaat acgcacgccc attttttcac taagcgggat atgttcgtac cacgcctgtt
    10501 gcagctgcgc gcaccagtcg ccgcgatgca aaatatcatc cagagtggcg acgggcttaa
    10561 tcatcaaaaa atggcgaatc ggcgtggtgg ttggcgtggt gatttctccc tgattaacaa
    10621 accccagctt ggcgaaaaac tccaccgcgt cttcacgggc gctacaggtc acgcgcttaa
    10681 cgccttcctg acgcgccacc gactccaggg tcatcgccat cagcgttcct aaccctttgt
    10741 cctgcacgtc cgggtgaacg gccataaagc gaatggacgc ttcattgtcg gcattaatat
    10801 acagtcggcc taccgccacc agattaccct gctcgtcgac gaccatctgg tgatgcgcca
    10861 tcgcatccca cgcgtcgcgt tccgaacctt ttggttgatg caggggctta cgcaacattt
    10921 cccagcgaaa ctgatagtaa cgctctaatt cttcttctgt ttgtggaacc cgaaggtgat
    10981 acatagcgat actctctctt gtttcccgtg acaaccctgg aagctggctc atacctgcaa
    11041 ccagaatgtc acggggccat cattgaccag cgatacctgc atatcggcag cgaagcgtcc
    11101 tgtttgcgtg ttcatctctt gctggcggca gcgttcgacg aaatagtcat ataacgcctc
    11161 tgcgcgatcc ggtgatgcgc ctttggagaa acttgggcgc atccctcgtt cggtatctgc
    11221 ggcgagggta aactgggaaa ccaccagtac actgccgccc gcctgttgca cgttgagatt
    11281 catcttgcct tcggcatcgc taaagatgcg gtagccgagc acacgctcgc acagacggtt
    11341 tgctttctgt tcgtcgtcat ccttttcgac acccaataac accaaaagtc ccgcgccaat
    11401 ttcgcccgtc acttctccct ccacggtgac gctggcacgg gttacgcgtt gaattaatgc
    11461 aatcatggtt cgtcgtcttc ttcttgttca gctgcttgtt ttagtttgcg gtattccccg
    11521 agagtgacag taatttccgc gccaagcaag acgatacacc atgtccagta gacccaaaca
    11581 aagagaatgg ggatcaccgc cagcacgccg taaatgagct gatatgacgg gaacatggtg
    11641 atataaagcg cgaaaccttt ctttcctgct tcgaacagga gtgcagcgac aaacgcgccg
    11701 acaatcgcgt cgcggttagg tacgcggatg gtaggaacaa tgctgtacag caaccagaag
    11761 gagatccacg acaacagcag cggaaaaata cgcagcacgt tgtcgatgac agtattgaga
    11821 tcgctcgccc agcgcaggga gagcaaatag gaactgatcg ccagactggc ccctgccagc
    11881 agcggcccca gcgttaaaat catccagtac acggcaaacg agtaaatttt tggtcgcgct
    11941 cgtttactgc gccagatggt attcaacgcg ctatcgatgg agtacatcaa caataatgcg
    12001 gtgacgatca gcccgcacgc gccaacggcg gtcatcttgt tggaattggc aacaaattgt
    12061 tcgatatacc gctgaataac atcgccagta gcaggcagaa agttggcaaa aataaagtga
    12121 cgcaactgaa tgctgacgtc ggaaaacatg ggaaaagcgg cgaaaagcgc aaaaacaacg
    12181 gcaaccagcg gcactaatga gagcaaagac acataggcaa ggttacctgc cagggttgtc
    12241 atgttgtcct catcaatacg ttgccagagt agttttagcc aggcccatag tggacgagta
    12301 cggtgcctgg ctttgtcctg aatggttttt agcataacac cttcgcgaaa tagtccggga
    12361 tggtggtttt atctttcacc agaatactgg taatgcccag ctgattggct ccttctatat
    12421 tatcggcgtt atcgtcgaaa aagaccgtat cgctgggtga aaaaccttcc gcctgcaaaa
    12481 catgctggta aattcgtgct tcaggtttgc gcatccccag atcttgcgac agatagatat
    12541 ggtcagcagc atcacgaatt tccgggtatt cttccggcca gaaggtggta tgcaggcggt
    12601 tggtattgga aagcaccacc acgcgatgcc cctgctcacg cagtttatgc atgatggcga
    12661 tcacttccgg gcgcagcgca acaaacaccg cctgccagcc gtgagagaac tgctcgtagc
    12721 ttagcggtag agccatctca tgacacagcg cctctgcgaa cgcttcgtcg ctaatttccc
    12781 cacgctcatg ctgatgaaac gcctccccca tatgaaaact cttcttaagc gatgccagcg
    12841 gaatacgcgt taaatcgctc caggctccca gcacacggtt aaagtcgata tcgacaatca
    12901 cattacctaa atcaaagata tagagcattt ttgcctcctt tcgcgccaca agaaattaac
    12961 tgtagcggga aaggatgggt ttggctatgt ggcaataagg gtaatcttgt gatgctgatt
    13021 gcgaaaaagc agagtaattt cactggcttt ttcgcaatag atgaatggtt aagattcagg
    13081 tgtaaattgc caacgagttt cgatgaccag cggcctatct ggtgtcacca attccggcgt
    13141 tagatttatt gcgttaggtg cctgagtcag cgggttaata caggtggcat cgggctgttt
    13201 atcaaatacc accagactgt ttgcagaaga gatcatggtc atcgcgagtt taccaggcca
    13261 cagtaactta accttcaccc cgtcataaaa accaaaacag tcatcccagg gaccggcttg
    13321 aggagagata cgatttccag ttggcagttc atcactaccc gcttcttctt gccagtccgc
    13381 atcaaaaagc acctgcaacg attctgtatt ttgcggagtc agctttttcg caaaccacgg
    13441 atgccagcct gcggaggccg ggaatgtatc ggcatatgaa tggacttcca gttgcaaaac
    13501 cagcgcatcg ttctccagca aaaaagtctg aatcacttcc ccttgccagg gccacggtga
    13561 agccaggggc atacgtaaag tcagggaatc tgctgttttg tcgatgattt cccacgttga
    13621 gtaacaagcc ataccatgca acgcatgtgg tggtttattg gcgggtaaag aataacattg
    13681 cccaccagca ttcagcgtcg cattccccag tcgccctgcc cacggcacca ttgggaaaca
    13741 gccatactga aaagctctgc gctgcggctg ccattggcgt aatacttcag aaccaaatgc
    13801 ctttaacgag gttatcctgg caccatcatg cggataaatc tgcaggtgaa tattgccttt
    13861 ttccagcgtc actaactctc ctacaggccc aggccatggc gcgtcaaacg cctgttgtag
    13921 cgccaggtaa tgctcgtttt ttcgttcagt tttcatgaga ttgttccctg atgtagcggt
    13981 aagaagcggt ttcttgttca tcggtcggct caatcagcca gacgcaaaca aaggaaagaa
    14041 ctgagacagc aaataaaaac agagcgacat accacggttt tcctccccct agcgccagca
    14101 aacttgaggc gataaggggg cttagtccgc caagaatcgc ggaaaattga taagcaaaag
    14161 aaaggccggt atatcgtact ttggtaccga acatgcgggt gaaaagtgtt ggctgcacgg
    14221 caaacatcat cgtcggacct aagttgtagc cgaggatcat actgcaccag atgatgagcg
    14281 tacttttgct atccagtaat aagaagaaag gaaacgcgaa caggacacaa aatcctgctc
    14341 ccgacaaata cagacttttt tgacctatac gatctgataa ccatccaacc aatggacaac
    14401 tgaaaatacc gatcgccgag gcaatcaaca tacccgtcag gggaatatct cgcgacaatg
    14461 ctaattggct ggaaatatag acaataccaa aggcgttaat aatattagag gagacacttt
    14521 ccgccagcct cgcacccagt gccaataaaa tatttcgtgg atgttttttg aataattcaa
    14581 tcaacggagg aacggacttt tctttattat tcgtcgtttt ttgcttttga aaatccagcg
    14641 tttcttcggt atgcaggcgg ataaacagcc caacgatcaa catcaccgca gaaagccaaa
    14701 acggaatgcg ccatccccag gagagaaact gctccgaagt aagaaaatgg ttacagagag
    14761 caaaaatacc ggttgccagc ataataccca cagacgcagc cgtttgcggg agtgaaccta
    14821 aaaatccgcg tcgggattcg ggcgctgatt ctatggtcat caatgccgct ccgccatact
    14881 cgccaccaag accaaagccc tgaattaaac gcagcgccat taatatgagc ggtgcccaga
    14941 tcccaatttc ctgatacgtc ggaataaaac caatcaaaaa tgtggagcaa ccgacaatag
    15001 ccagtgtcca gataagtgtt atttttcgcc caattttatc gccaaaatgg ccaaagacga
    15061 taccacctaa aggacgggtc aaaaaaccaa cgccaaaggt agcaaaggcg gctataagac
    15121 ctataaaagg atcgctgtct ggataaaaca gcggtgcaaa taccagtcct gctgccgtgc
    15181 caaaaataaa gaagtcatac cattccatta acgcgcctgc gaaactgccc ataacgacac
    15241 gtctcatggc tttaccggaa agcccttcac gcggtgcggg taacttttca gacataattt
    15301 tcctctcctg gtaggcgaga aatctgggat acgaaccact aaacgtgggc agtttgaatt
    15361 aatgcgcatc ggtttaagcc gatgcgcatt aatttatttc ttcaattcag ccagaacctc
    15421 tgcatcctca gcatcaacat ttgcacccag tggttcccaa ttaatcaata cgcaggtcag
    15481 gaaccccaag aatggcacga ttgccaacca gtaaaacgct tcagttttca acgcccccca
    15541 cagcatcggc cagaaaatga gtcctgcaat tgcccctgtg cgcataatag cgcgggcaaa
    15601 tccgacccca gtagggcgaa tagcgggagg ataagagagt gtggcaatgg tcatgcccag
    15661 cccaccagga cccgctgaat ggaagaaaat aattgatccc aataacccta aagacagcca
    15721 tggattagtc gttgccagtg cgcctaacga cagcaatgct aaggtcacaa cagcaaatcc
    15781 atacatcgac tgtcggcgag tacccagtcg ttggagaata aacgagccga tcatcccgcc
    15841 tgtcacccca caaagggcat tcacgacggc agaacccgtc aacgcaccag taagcccgcc
    15901 acttattatt cccgccaacg taagaggaag ataaacacct acggcgttat attgccacgc
    15961 ctgcatggtt gccacgacac agccaagaat ggtgcgtttg cggtaacgat cattaaataa
    16021 gatgccatat ccaccactgt atttattctc tgctttttcc gagaatttcg cttctgttgt
    16081 actcgatgcc gcaacgcgag cctgaacacc ataacgtttt cccagaatgt cgcacgcttc
    16141 ctggtaacgc ccgacgcgtg cggcccacat tgcggattca ccaataaaga agtaacgcaa
    16201 aatcatgaag atgactgcaa aaatagctcc gatgaatatt ccgtaacgcc acaattgttc
    16261 ttcagcgata cccgacagaa gcagcggcaa gagcaccaaa taaacaacag ttgtcgaaac
    16321 ataccaggcc atttgccaga ggttgaccga cgttccggtt ttcttcgacg ttgaaccacg
    16381 aagctctgcc acggcattgg ttgccaaagg gaagtcgatg cctaaaccaa agcccatgat
    16441 gacgcggcac accagcaccc accagatact gggcgcaata gctaccgcag cagccccgat
    16501 ggtgcacagc cccatgccaa taataaatgc tcgcttttgc ccaaagcggt ttattactcg
    16561 gttcgctaat aaaccgccaa ttaacgcggc gattaatact gacgcattaa cggttgcggc
    16621 aaggcctgga gaaatgccaa attgagcggc aatgtattta ttaccaaacc ccaccatcgc
    16681 cgcctggtag gcatcaatca atatgccgcc caaagcaata aaaataacgc cgatactggt
    16741 gttgattgca gagttcttat taacaatatc aattacgtcc tgtggccgac taattgaata
    16801 agttgcagga ctggtgattt gactcatcgc agtgtccttt ttaattgttg tagggtggcg
    16861 ctgacgtgac gcagcgccag acagagattt agaaaatcat tccggcgcaa attgcagcat
    16921 gacctttatg gagtcatttt gctgcgcgcg ttcgaatgcc gccgctgcat cctcaatggc
    16981 aaataaatcg gtaaccagtt cactggtatc aaaccgccca tccgccagcc attcgataac
    17041 agcatcgaaa tcttcaggga tgtacatgcc agagttgagc aagtcacgct cgaaacgttg
    17101 catcatcggt aatggtattt cacgcggccc acttggtacg cccatgcaca cgacggtgcc
    17161 tccggcatat acacgggtac atgcatcgtt aagcgtggcc tgcgctgcaa cgacatcaat
    17221 cgcccccgta aatcgcacat ctgcggccag ctcacctggt gcccaaactt ctgctgcgcc
    17281 cagctttaac gccagcgcgc gtttggcagc gtcaggttca atcacggtaa tcttacctgc
    17341 ccccataatg cgcagtgcct gcacgatgga taagcctatc gttccggcac caatcaccag
    17401 tacgctttcc agagaagctt tcggcattcg gttaacgcag tgacgggcac aagccgccgg
    17461 ttcggcaaag gccaacactt ttagcggtaa agaggctggt gcgacatggc aattacgcgc
    17521 aggaacaatg tgttgtgagc gagcaaagcc cggtgcgcga aaaccagcaa cctgcggtgg
    17581 ttcacaaaga ttaaaacgtc ctgctttgca ggctcggcat tccatgcaag ccatgatggg
    17641 atcgaccaca acatgatcgc ccggctgtac atttttaacg tcgctgccaa cttccgtaac
    17701 gcgcgctgca atttcatgac cggggacgac aggtggttta gcaaacggat gaccgccttt
    17761 cagaacatgc agatccgaac cgcatatccc atagaacaca ggcgcaatcc gcacttcgtg
    17821 cggtttaagt tcctggtgtg gtacttcaac atattgagtg gtgactttcc caatatctga
    17881 aaataaaacc tgggtaattt tatccattgt tattctccag aatcgatatc cataggggat
    17941 tagcgttgcg cgataccttc atcgacaatt tctgccaacc gttgtaaacg aggaacagag
    18001 acatcgcgca gcatagtttg tggatcttgt gcaccaacaa ccgacgccca aaccgctcgt
    18061 ccagccagga agcctgacgc accacctttc atggcaatac ttactgcacg gccaaaaata
    18121 tcggcatcaa cgccagaaga aagaattacc cacggcatct tcatctgatc gttaagttgc
    18181 tgacaggcgg caagtaaggt tttttcatcg cctttgcccc ctaatggcat ctcggctttg
    18241 taaagatcag cctctgttcc accaagctct gcggcggcgg caacaatggc gctttcacga
    18301 tcaaagtccc aaccgcgacg aggtggacgc accacaggtt caataatgct aaccagcccg
    18361 gcactacggc agcgacgaac aaatttatca accattgcca gacgttcctc tgcgggttca
    18421 tcttcacgcc agagtacaag taatttcatt gccttagccc ccatttcacg ggctttatgc
    18481 gggtcgacgg acatatctat ttcaacgcta tcaaccggga tgccattacc tgggataaaa
    18541 agatcggcag cgacaatcag gccgcaagaa ttagcaacag cgccctgctc gactatttgc
    18601 tcaagacaaa attgtttatc cgccagaacg gcagaagcat aaggagaaag gattcgggtc
    18661 gcggcaactt taaaatcggt gagtacactg tcggcgatgg gttttggctg ccctgctgcc
    18721 gcaaacatca agcgcatggc ctcacgctga tcgacagcta acatggcaaa tccaccacct
    18781 gggcgggaga tatctttcag cgtaaatggg gtgaagtggg atgacataga aactcctgtt
    18841 aatagataaa attaaagacc agcctgctgg cgaacctgat ttagcagggc actccagtcc
    18901 tgccgccctt tgccgtcttg acttgctgca gcgtaataac tttctgctgc aatccccata
    18961 ttgagcggtg cccccgtctc ccgagcgacg tcaactgcaa tacgcagatc tttcaacgcc
    19021 agatccacca tgaaggcggg tgaaagatcg cctttcagca ctttgcaagg ccaggtggtc
    19081 gtaaaatgtc ctttccctgc tgccgtacca ctcatcacct taattgcgac atcaagattt
    19141 aatcccagtg actcacataa cacagcggcc tcggaggaca gcgcattaag cgcaatgctc
    19201 atatagttgt taattagctt cacgcggatc cccattcctg ggccgccagc ctcgaccagc
    19261 tcattaccca tgcacatgag aatcgggcgt gcacgtgcaa tctgctcatc agtgccaccc
    19321 gctaaaatca gcaatgtgcc atcaatcgca ttcacggacg ttctgcccac gggggcgtcc
    19381 atcatactga tgcctttttc ttgtaactcc cgtattaatg catcggtctg taagggatga
    19441 atagtcgaca tatcaatcaa taacgcgtca gatgaaacag cctcacagac gcctttttcc
    19501 cccaacagaa cctgtcgaac aatgtcacca ttgggaagca tagtgatgat aaattcagcg
    19561 cctgctgccg cttccgctgg ggtttgtgcc gcagtggccc cctgcgtaac tagcgcatcg
    19621 acggcctgtg cattgacatc aaatacctgc aatgaatgcc ctttttgcaa tagattgtta
    19681 gccattgggc tacccatctg ccccagcccg agaaatgcaa tggttgccat gaatactctc
    19741 ctgtctgaca ttgacaaaat aatgacaatt gaaaattcgt tttcgtctgt ttttgagcat
    19801 atttgtaatt tatgttcaaa aacacgacac gcatcactaa tttgatcatt tgtgtcattt
    19861 aaattgaccg aagacgacaa aattaaacag gacaaggcaa atatgaccaa aattgcatgc
    19921 gtgggtatca cagttcagga caggatttat tctttgctca ctctgccgga tggcggcgga
    19981 aaatatcagg caaatcatta tcttgagatt ggtggcggcc cggcagcaac agccgctgtt
    20041 gcaatcgcaa agcttggcgt agaggttgat ttcatcggtc gcgtcggcga tgacagttgt
    20101 ggaaatacct tacttgcaga acttgagggt tggggcgtca ataccgcatt ttgccgccga
    20161 tatcctaacg cccgttcatc gcaatcagcg atcctcgttg atcagcatgg cgaacggatt
    20221 atcgttaatt atccaagtcc cgatctcggt actgatgccg aatggttaga agcgatcgat
    20281 ttctctcgtt atgatctgat tctggccgat gttcgctggc atagcggtac tgaaaaagca
    20341 ttctcgctag cgcgtcttgc gggtgtgaca acgcttttag acgccgatat gacaccgcag
    20401 gatatttctc cacttgtcgc gcttgccgat cacgccgtgt tctcaacacc agggctgaaa
    20461 cgcatgactg gcctgcagtc acctgaagaa ggtttgttcc aggcaacgac acaaacagca
    20521 ggtaaagtgt atgttacgct gggttctgaa ggatcgttgt ggattgaaga tggtcatttg
    20581 tgtcaacagg aggcattttc tgtcaatgtg gttgatacca ctggagcagg cgatgttttt
    20641 cacggcgcac tcgcagtagc gttggcagaa aaaatgccaa caaaagaggc tattcgtttt
    20701 gccagtgcgg ttgccgcgat gaaatgtacc cagcctggcg gtcgggccgg cattcctaat
    20761 cgtgagcaaa ccgaatcatt tttgtcactt tatgcgtaaa atggtaaaca tctaccgagg
    20821 aggggcaagg gagtattcat gagtatcatc gaagtgacag gtaatccacg ccatgaccaa
    20881 ctcgttcatc taatcgcaga acgcggttat atgaatattg aagaactggc acaactgctg
    20941 gatgtttcaa cgcaaaccgt gcgccgggat atccgaaaac tcagtgagca gggcttgatc
    21001 acccgtcatc atggaggggc cgggcgcgtc tccagcgtca tgaataccgc ttttgagcaa
    21061 cgggaacttt cgctcaccgc cgagaaacgg gcgatcgctg aggcagtcgc cgattacctt
    21121 cccgaacgct gtaccgtctt tatcaccatc ggtacaaccg tagaagccgt tgccagggca
    21181 ttactcaacc ggcgtgattt acgcattatc actaacagcc tacgtgtggc acagattctt
    21241 tataagaatc aggatattga agtgatggtg ccgggaggca ctttacgcgc tcataacggc
    21301 gggattatcg gcccaggagc cgtggatttt attgaaggct tccgcgcaga ttatttaatc
    21361 accagtatag gtgccattga acatgacggc accctactgg aatttgatgt aaatgaagcg
    21421 ttagtcgcga gaacgatgat taaacatgcg cggaatacat tgttagtggc cgatcatacg
    21481 aagtttgccg cgtctgctgc cgtttcaatt ggcaatgcac ggaatgtcag ggcttttttt
    21541 acagatgccc cgcctcccaa ttctttctgc cagttgttaa gtgaagagaa tgttgaactg
    21601 gtggttgccg agcaggaagt ttcctgaaat tgaaacttcg aaaaatccgc tggggattca
    21661 gcggatttta ttttgccgga tgcggcctac ggttgatacg gttttcgttg gccggattaa
    21721 gcacatccgg ccattaatta agcttcagcc gccagcatct ctttcttacg ttggctaata
    21781 cgcttaacca gcattacgga aaccaccgca ccacagaatg cgaagcaggc cataatgcca
    21841 aacacgattt tgtagccaat aatccccgga tttaaatcca gaatgtagcc atacagactg
    21901 aagcagaaca tcgccggagc gtaaccaata aagctaccca acgccatcgc cgcgcctgtt
    21961 ttattttcag caatttttgc ttcgccgata ggtgcaaaaa atacggctcg ctgtgtaaag
    22021 actatcgcgc caaagcccag cgtacatgcc atccctaaat agaccggcat actttcgtgc
    22081 ggcagcataa tcaacagtac gagcgcagcg gtactgatga taaaggtgta gcatagatat
    22141 ttactcggcg atttcaggat cttatctgaa atcatgccac caatcggtcc accaatcatt
    22201 ttcagacagt attggttaat gatgccgtaa gcccccacca gcgcaacggg caatagatag
    22261 atgtttttca ggaatggaat gaagaatgtc aggccacagt aaaccgcgta aacgaagaag
    22321 acgttaaaag cgataagcca gatggttttg tctctcagca ccgaggtcat actggtgttt
    22381 tgcgatgctc cgtcttcttt tttcacctca acggacggtg cctcttcttt gtcattcagg
    22441 acaaagaaaa taataatgcc tacggcaatc acaataaggg aatagaacca gatgcctgct
    22501 ttaaaaccca ataagccact gccaaaccag gtaaatactg ccaacgcaga aaatgccacc
    22561 acggtatcga caatgccacg ccctgtttcg aagaagccaa acaaccgacc ttgttgttcg
    22621 ctattgccca atcgacttac cgacttgagc aagaccggcc agttcatcat gtcgcaagta
    22681 acgccaaata gcgcccagac aaagaggatg ccccaatacc ccggcattgt cgtcaaataa
    22741 acacccagta atcctgtcgc aatgagtgag aatgacatgg tgtatctgcg cggtagttta
    22801 tcggcaaaat aaatagacag aaaaaaaccc actgtcgtga caaatgagtt taccgacata
    22861 gcattaccaa tttgaccatt ggtcaaatgg aaatattcct gcatcgggat ataaaacgca
    22921 tctttcagcg acggtaattt ataaattgta ccgccacaca atgttaatag actaaataac
    22981 gcccatttct ttttcgtgag cataacatcc cctaagcgat ttttaaaatc actgttttaa
    23041 aggcaataag ataccgacca ttcatatgga atggttttat taactgcgta tgagttaaaa
    23101 cttattagtg gttcagaaga tggtttgcct cttgtttgat ttgttgtagt acgttacgca
    23161 catgatttat ctgattaatt gcatcttctt tttctttacg taaccgcgca tccagtaaat
    23221 gattttctgg caatggtgtt tcactcatct ggcgataagg gatccacgga ttatcatctt
    23281 cgtcttcaaa gcggaacgcc ggcgcatagt aatcaacctc ttcttccagg ccatatgccg
    23341 tcacctgcgg ctcatcatca cccaggcata taaggtgcgt taataacgct ttgaacggca
    23401 tatcaccctg acctgaaata caggctttat gacccaggcc acccggttct ttaacgatca
    23461 aggcatcttt gatatggacc tgggtaatat gcggcgccat cgtttttaaa gcgtcgatgg
    23521 gatgctcatt tgcattgatc atgttcgcaa aatcaaacag taaggaaaga gattccatct
    23581 cactttcttt gaccagcgac accagctcat ggctttttaa atcttcatgc tgttcgatcg
    23641 taaaagtcag gccgctgtcc tgatacgttt cccgtacata ggcaatgtcg ttagcgatga
    23701 tcgataatac gtcgcgcaaa ttaccttcat aacgtggata aaaacgtacg gacgatgccc
    23761 cagttttcaa cgcaatggcg acggcttcgt caatagatgc cttatctgag gcgctggttt
    23821 caatatgaat atcaaggttc agacggcggg ctttatctcc aaaggcagag agttctttat
    23881 cgtccatatt accaagagaa aaacgctcgc catccagaac atggattttg acgccacgta
    23941 gattattttc acttgcgata tcaagtaaat cagcaggcaa cactctttcc agccgcatat
    24001 ttaaatgaaa ggcataagca tgtaaataaa gaggaagagt gtcgacacgt tgtagaatct
    24061 ttcttgcgtt atttattgtc accataaaac acctacagat taatgatgag gtctacggcc
    24121 tcaaatcgcc aatattcaat atctatctgg acaatattgc cttgctggtc ggcgcgatgc
    24181 ttttcaatta agatcgctgg catacccgca gaaccgccaa gatacttgct gatatcgccc
    24241 ggcattctga cgggtttgaa aatcaatttt tttactaccg tctctttttg gtagcacttt
    24301 tcccagactg cagaaaatga gtggttttca agttgttcaa taaaacccgg agcaacttca
    24361 ggattaatat atgtttcgtg atagaaaact ttatgtcctt ccagcgcccc ccagccggtg
    24421 atgcgataga gttcagttga tggtgtcact gcaatcagtg gcgcgagcgt ctcgggaata
    24481 tcgctggtac ggtttttctc ggtaaacccc caggaaggct ctcgtccttg ctcaattgcg
    24541 gcccgctgaa agctcgccga caactccgga ctgtaattaa atcgcggctg ggtcacgaac
    24601 caccccttac gatccttacg gaagattttg gattctgcct cgaggttcaa caacgcctgg
    24661 cgcagcgtca tacgtttaat gcccagcagt tctcccagct ctctttctga agggagtttt
    24721 ccacccggcg tggtgatgcc atcttttaac caccgatcca gtttctcttt tgcattttct
    24781 acggtggatt gatttcccgt cattgtttaa tccttttggt ttaaaccaga tgaggcatct
    24841 tacgcttacg ccaaaaaaga aagcaattaa cggcgtatga ctttgcagat aattgtgaca
    24901 aagctctcta ttgacgtaat cccagactta taagggagat actcgcattg gtgtgcgctc
    24961 acgacaggta aaaaaaaacc tgccagcgat ggcaggtttt ttataactaa aaaacgaaat
    25021 taatcgtctt tcggtgcgcg gttggcgcgg cgacgatcgt tttccgtcag gtgacgttta
    25081 cgaatacgga tagaggtcgg agtcacttct accagttcgt catcatcgat gaactccaga
    25141 gcttgttcca gagtcatgcg gataggcgga accagaacaa cggcttcgtc agtaccggaa
    25201 gcacgcatgt tggtcagttt cttaccggtc aggcagttta cagtcaggtc gttagagcgg
    25261 ctatgaatac cgataatctg accttcgtaa acttctgcac cgtgaccgag gaacagctta
    25321 ccgcgatcct gcagaccgaa cagcgcgaac gcgaccgctt taccctgacc gttagagatc
    25381 agtacgccgt tctgacgctg acccacttca cccggacgta cgtcgtcgta gtggctgaag
    25441 gtggagtaca gcagaccagt accggaagtc atggtcatga actcagaacg gaagccaatc
    25501 agaccacggc ttgggatcac gtagtcgaga cgtacgcggc ctttaccgtc tggattcatg
    25561 tttttcaggt cgcctttacg ttcgcccagc gcctgcatta cagaaccctg atgctgttct
    25621 tcaacgtcca gagtcacgtt ttcatatggc tcttgtttac gaccgtcgat ttcacggaag
    25681 ataactttcg gacgggatac cgccagttcg aaaccttcac gacgcatgtt ttcgatcaga
    25741 acagacaggt gtagttcgcc acgaccagaa acgcggaacg catcagcgtc ttcggtttct
    25801 tctacgcgca gcgcaacgtt gtgtaccagt tctttgttca gacgatccag gatctgacga
    25861 gacgttacga acttaccttc tttaccgcag aacggcgagg tgttaacgca gaagaacata
    25921 gaaacggtcg gctcatcaac ggagagtgcc ggcagcgctt caacgttttg cgtgtcgcaa
    25981 acggtgtcag aaatgttcag ttcgccaagg cccgtgatcg caacgatatc gccagcttcc
    26041 gccaggtcgg tttcgatacg ttccagaccg aggtggccca gcactttacc gactttcgcg
    26101 ttgcgggttt tgccttcgct atcgatgata gtgacctgct ggttcggctt cactttaccg
    26161 cgcttgatgc ggccaatgcc gataacgcca acatagctgt tgtaatcgag ctgagaaatc
    26221 tgcatctgga acggaccgtc aaggtcaacg tccggcgcag gaacgtggtc aacaatcgcc
    26281 tggtacagcg gggtcatgtc ttccgccata tcttcgtggt ccagacccgc gataccgttc
    26341 agcgcagaag cgtaaacgat cgggaagtcc agctgctcgt cggtcgcgtc gaggttaacg
    26401 aacagatcga atacctgatc cacaacccaa tcaggacgcg cgccagggcg gtcaactttg
    26461 ttgataacaa caatcggctt caggccgtaa gcaaacgctt ttttggttac gaagcgcgtt
    26521 tgcggcatcg ggccgtcaaa tgcgtcaacc accagcagca ctgagtctac catggacatt
    26581 acacgttcaa cttcaccacc gaagtcggcg tgccccgggg tatcaacgat gttgatacgg
    26641 taatcattcc atttgatagc ggtgtttttc gcgaggatgg taatcccacg ctctttctcc
    26701 aaatcgttgg agtccatcac gcgctcttgg gtttcggcac gagagtcgaa cgtaccggat
    26761 tgttggagca gcttgtctac cagggtggtt ttaccatggt ctacgtgcgc gatgatggcg
    26821 atattacgca atttttcgat cacaactttg cctcaggcat tagaaatagc gcgttattgt
    26881 acacggatta atcgcactac aaaacaggat cacaaacatc ctccgcaaac aagtattgca
    26941 gagtcccttt gtgatcgctt tcacggagca taaaaagggt tatccaaagg tcattgcacc
    27001 aacatggtgc ttaatgtttc cattgaagca ctatattggt gcaacattca catcgtggtg
    27061 cagccctttt gcacgatggt gcgcatgata acgcctttta ggggcaattt aaaagttggc
    27121 acagatttcg ctttatcttt tttacggcga cacggccaaa ataattgcag atttcgttac
    27181 cacgacgacc atgaccaatc caggagagtt aaagtatgtc cgctgaacac gtactgacga
    27241 tgctgaacga gcacgaagtg aagtttgttg atttgcgctt caccgatact aaaggtaaag
    27301 aacagcacgt cactatccct gctcatcagg tgaatgctga attcttcgaa gaaggcaaaa
    27361 tgtttgacgg ctcctcgatt ggcggctgga aaggcattaa cgagtccgac atggtgctga
    27421 tgccagacgc atccaccgca gtgattgacc cgttcttcgc cgactccacc ctgattatcc
    27481 gttgcgacat ccttgaacct ggcaccctgc aaggctatga ccgtgacccg cgctccattg
    27541 cgaagcgcgc cgaagattac ctgcgttcca ctggcattgc cgacaccgta ctgttcgggc
    27601 cagaacctga attcttcctg ttcgatgaca tccgtttcgg atcatctatc tccggttccc
    27661 acgttgctat cgacgatatc gaaggcgcat ggaactcctc cacccaatac gaaggtggta
    27721 acaaaggtca ccgtccggca gtgaaaggcg gttacttccc ggttccaccg gtagactcgg
    27781 ctcaggatat tcgttctgaa atgtgtctgg tgatggaaca gatgggtctg gtggttgaag
    27841 cccatcacca cgaagtagcg actgctggtc agaacgaagt ggctacccgc ttcaatacca
    27901 tgaccaaaaa agctgacgaa attcagatct acaaatatgt tgtgcacaac gtagcgcacc
    27961 gcttcggtaa aaccgcgacc tttatgccaa aaccgatgtt cggtgataac ggctccggta
    28021 tgcactgcca catgtctctg tctaaaaacg gcgttaacct gttcgcaggc gacaaatacg
    28081 caggtctgtc tgagcaggcg ctgtactaca ttggcggcgt aatcaaacac gctaaagcga
    28141 ttaacgccct ggcaaacccg accaccaact cttataagcg tctggtcccg ggctatgaag
    28201 caccggtaat gctggcttac tctgcgcgta accgttctgc gtctatccgt attccggtgg
    28261 tttcttctcc gaaagcacgt cgtatcgaag tacgtttccc ggatccggca gctaacccgt
    28321 acctgtgctt tgctgccctg ctgatggccg gtcttgatgg tatcaagaac aagatccatc
    28381 cgggcgaagc catggacaaa aacctgtatg acctgccgcc agaagaagcg aaagagatcc
    28441 cacaggttgc aggctctctg gaagaagcac tgaacgaact ggatctggac cgcgagttcc
    28501 tgaaagccgg tggcgtgttc actgacgaag caattgatgc gtacatcgct ctgcgtcgcg
    28561 aagaagatga ccgcgtgcgt atgactccgc atccggtaga gtttgagctg tactacagcg
    28621 tctaatagtt gaagttgtac tacccggcgc aacaacgccg ggatttagtt gccgtggaaa
    28681 tttcagccca tctctgcatg ggcttttttc tccgtcaatt ctctgatgct tcgcgctttt
    28741 tatccgtaaa aagctataat gcactaaaat ggtgcaacct gttcaggaga ctgctttatg
    28801 gcaacaggca cgcagcccga tgctgggcag atcctcaact cgctgattaa cagtattttg
    28861 ttaatcgatg acaacctggc gatccattac gccaaccctg ccgcgcaaca actgctcgcc
    28921 caaagctccc gcaaattgtt tggtacaccg ttaccggaac tgttgagcta cttctcatta
    28981 aatatcgagc tgatgcaaga aagtctggag gcggggcaag gttttaccga taacgaagtg
    29041 acgctggtca tcgacgggcg ctcgcatatc ctttctgtga cggcccagcg tatgccggac
    29101 ggcatgatcc tgctggagat ggctccgatg gataaccagc gccgcttaag tcaggaacag
    29161 ctacagcacg cccagcaggt tgctgcccgt gatttagtgc gcggcctggc acatgagatt
    29221 aaaaatccgc ttggcggttt acgtggcgcg gcgcagctgc tcagcaaagc gttacctgac
    29281 ccatcactac tcgaatatac caaagtgatt atcgaacagg cggaccggct gcgaaatctg
    29341 gtcgaccgtc tgttggggcc gcagctgccc ggtacgcgcg ttaccgaaag tattcacaaa
    29401 gtggctgaac gcgtggtaac gctggtgtcg atggaactgc cggacaacgt gcggttgatt
    29461 cgtgattacg atcccagcct gccggaactg gcgcacgacc cggatcaaat tgaacaggtc
    29521 ttgctgaata ttgtgcgcaa tgcgctacag gcgctggggc cggaaggcgg tgaaatcatt
    29581 ctgcgtaccc gcaccgcgtt tcaactgacc ttacacggcg agcgctaccg gctggcggcg
    29641 cggattgatg tggaagataa cgggccgggc attccgcctc atttgcagga tacgctgttt
    29701 tacccgatgg tcagcggccg cgaaggtggc accgggcttg gcttatccat cgctcgtaat
    29761 ttgattgatc agcattcagg caaaattgaa tttaccagtt ggccagggca taccgagttc
    29821 tcggtttacc tgcctatcag gaaataaagg tgacgtttat gcaacgaggg atagtctggg
    29881 tagtcgatga cgatagttcc atccgttggg tgcttgaacg tgcgctcgct ggggcaggtt
    29941 taacctgtac gacgtttgag aacggcgcag aagtgctgga ggcgctggcg agcaaaacgc
    30001 cggatgtgct gctttcagat atccgtatgc cgggaatgga cgggctggcg ctgctcaagc
    30061 agattaaaca gcgccatcca atgcttccgg tcatcattat gaccgcacat tccgatctgg
    30121 atgctgccgt cagcgcctat caacaagggg cgtttgatta tctgcccaaa ccgtttgata
    30181 tcgacgaagc agtggcgctg gttgagcgcg ctatcagtca ttaccaggaa cagcagcagc
    30241 cgcgtaatgt tcagcttaac ggcccaacga ccgatatcat cggcgaagcg ccagccatgc
    30301 aggacgtgtt ccgtattatc ggtcggcttt cgcgttcttc tattagcgtg ctgattaacg
    30361 gcgaatccgg caccggtaaa gaactggtcg ctcatgccct gcatcgccac agtccgcgcg
    30421 ccaaagcgcc gtttatcgcg ctgaatatgg cagctatccc aaaagatttg atcgaatcag
    30481 aactgtttgg ccacgagaaa ggcgcgttta ctggcgcgaa taccattcgt caggggcgtt
    30541 ttgaacaggc cgatggcggt acattattcc tcgacgaaat tggtgatatg ccgctggatg
    30601 tgcagacgcg tttgctgcgc gtgctggcag acggtcagtt ttaccgcgtt ggcggctatg
    30661 cgccggtgaa agtggatgtg cggattatcg ctgccactca ccagaatctc gaacagcgag
    30721 tgcaggaagg taagttccgt gaggatctgt tccaccgcct gaacgttatc cgcgttcatc
    30781 tgccgccgct gcgcgaacgt cgggaagata ttccccgtct ggcgcgccat tttttacagg
    30841 ttgccgcgcg cgaactgggc gtagaagcga agttactgca tccggaaacc gaagctgctc
    30901 tgacgcgtct ggcgtggcca ggcaacgtgc gccagctgga aaacacctgc cgctggctaa
    30961 cggtgatggc cgccgggcag gaagtgttga ttcaggattt gcccggcgaa ctgtttgaat
    31021 caacggttgc ggagagtact tcgcaaatgc aaccggacag ttgggcgacg cttcttgcgc
    31081 agtgggcaga cagagcgctg cgttccggtc atcaaaatct gctttccgaa gcgcagccag
    31141 agctggagcg gacgttactg acgaccgcgt tgcgacatac gcaggggcat aaacaggaag
    31201 cggcgcggct actcggctgg ggccgcaaca ctctgacgcg taagttaaaa gagctgggga
    31261 tggagtgatt cacggcgtgt gtgtaaagat tgattattga gcgcaaattg ctggtatttt
    31321 gcgctttact gttccgataa gttcagtatg atcttgcccg gaaaacgggg agagtcatta
    31381 tgctggaatc aataattaat ctggtatcga gtggcgcagt tgacagccac acaccgcaaa
    31441 ctgccgttgc tgccgtgctg tgtgccgcaa tgattgggct gtttagttga gtttcagatt
    31501 gctgatgaag tgctctttgt tcatgccgga tgcggcgtga acgccttatc cggcctacaa
    31561 aagcaatatt ttcaacaggt tttgtaggcc tgataagcgt agcgcatcag gcaatcttgt
    31621 tctcagcaca ttgatggcgc tttcgttttc tactttgtaa acgaagcgcc attcactatt
    31681 taaatcaccc gtgagaactg ctgcatccgc gctttctggc gcagataggt atcaaagcac
    31741 atgcaaatgt tgcggatcag caagcgacct ttcgccgtca cctgtattcc cttctcatcc
    31801 acatccacca gcccatcttt tgctaacggg gcgagcagct tgagatcttc cgcaaagtaa
    31861 tcagcgaagt gcaaatccca ctgtttctca ataggggcgt aatccagacg gaagttgcag
    31921 atgagcgact taatcacatc gcggcgaata cagtcatcac gcgttagcgc aataccacgc
    31981 cacagcgcat tgccttgttc atccacttgc tgatagtact gcttcaactc tttctggttc
    32041 tgcgcgtagc agtcgccaat catgctgatg gcggaaacgc ccatccccag cagatcggta
    32101 tcgccctgag tggtgtagcc ctggaagtta cgatgcagca cgccttcacg ctgggccacc
    32161 gccagctcgt catccggacg ggcaaagtga tccataccga taaactgata gcccgattgc
    32221 gtcaggaagg cgatggtttc ctgcaggata tcgagttttt gctgcggact cggcaggtca
    32281 gcatctttga ttttgcgctg agcagcaaaa atggtcggca gatgcgcgta gttaaagaca
    32341 ctcagacgat cggggttcag ctccgccaca cgtttcaggg taaaggcgaa actctccggc
    32401 gtctgtttcg gcaggccgta aatcaggtcg atgttggtgg aggtaaaacc aatctcacgc
    32461 gcatggttaa gcagtgcaaa gatgaactct tcatcctgct cgcggttaac cagacgttgc
    32521 acttctttgt tgaagtcctg cacgcccatg ctcaggcgat taaagccttc ggcgcgtaaa
    32581 tgatcgagta catccagttc gatttcccgc ggatcgactt cgatcgaaat ctccgcatcg
    32641 gcattgaact ggaagttttc gcgcagcagc ttcatcaggc ggctgatttg cgctttattc
    32701 agatacgtcg gcgttccgcc gccccagtgc aattggctga cgtgacgccc ggcgaacagc
    32761 ggtgcacgat ggacgatttc ttgctccagc gcgtccagat actgatcggc cttgtgctgc
    32821 tggcgagtaa caatcttatt gcaaccgcag aagtaacaaa gcttatggca gaacgggata
    32881 tgtacgtaga gagataatgg acgctcagga tagcgcgcca cggcttgtaa aaacgcctgt
    32941 tcgccgaagt cttctgaaaa ctccagcgcg gtcgggtacg aggtgtatcg tggcccggaa
    33001 tagttatatt tctggatcag ggccagatcc cagtcgattt gctgtacaga catgctcact
    33061 ccttccgatg gcgtctctgg cggctacggc gtaccggcgg atgcccgttt cgggtcatca
    33121 gccggttgcg cagccacttt tgtcgccgcg acaaccgctg tagtttaacg aataaccaca
    33181 ccagataaca tataaccgga agggttatca gcagaacggg aaggcccacg gcggcaatcc
    33241 gttagttgcc ccgcagcaga cgcatcatgt cttcttgctt ctcgtcttct tcctcttctt
    33301 cgtcatcgtc ataagagagg ccgagtttct gcatcaactc atcaatacga tccagtttag
    33361 catccaccca ggattgctct tcggcgctca gggtttcgcc tgcttccaga cgttccagca
    33421 gcgcatccag acgctcatcc gtttccagta attccaactc cgcctgcggt gaaagcatag
    33481 gtttctcact cttcggtttg tgctgtttgg tgactttttc agtcacgccc aatggaatag
    33541 gggttttact gccaatacgt ggatcttttg gtgcgttctg gcctttgctg cctgacgtgg
    33601 tgtttccgcc cgctgcgcgg ctgcccggcg catgaccacg acgttttttc tgacgcttgc
    33661 gatcacgagc ttcctgatcc agctcctcgc gtgtttttcg acgcgctttt gcatgacctt
    33721 tgctgcgtga gtttgaagat gatggtttca taatggttcg tcttttagcc gttttattca
    33781 gtatagattt gcggcggaat ctaacagaaa gcaagcaaag aaaaaaggcg acagattact
    33841 ctgtcgcctt ttttcctgac tcataaccct taacgggtct tacaatcctt gcaggccgcc
    33901 aacacaccct gtttttcctt tagccggaac aatccgtgtt gattccattt ccgtttaatt
    33961 acgtctccgg acgtttgtct tcctgacaat cctctgtctt cgcctcctgg cgctcctgac
    34021 cctcatcctg agtcattgtt cctgttagcg tcctcgctct atgggtgcta ctttacgcct
    34081 ttgctttaaa caaacaatca accgccatca gtatttgcac attttcagag ttttatataa
    34141 aagcaattca ctgatttata tagcttattt gacatgcatc gcagaatttt ctctgctatt
    34201 tcgcataaag actatcggca atgtctcaca aagcgtatgg cttttactta caacgctcac
    34261 gccgaaaaat ccgcctttta ttatggttca ggtataatcg ccacaaagct ttgactgacg
    34321 gagacgaccg ctttgactaa tttgaattat caacagacgc attttgtgat gagtgcgcct
    34381 gatattcgcc acctaccttc cgataccgga attgaagtgg cttttgcagg ccgttccaac
    34441 gcaggtaaat ccagcgcgct gaacacgctg actaaccaga aaagcctggc tcgtacctca
    34501 aaaaccccag ggcgcaccca gcttatcaac ctgtttgaag tggctgacgg caagcgtctg
    34561 gttgacttgc ctgggtacgg ttatgcggaa gtcccggaag agatgaagcg caaatggcag
    34621 cgtgcgctcg gcgaatacct cgaaaaacgt cagagcctgc aaggtctggt ggtgctaatg
    34681 gatattcgcc atccgctgaa agatttggat cagcagatga ttgagtgggc ggtagacagc
    34741 aatatcgccg ttctggtgct gctgaccaaa gcggacaaac tggcaagcgg cgcacgtaaa
    34801 gcgcaattga atatggtgcg tgaagctgta ctggcgttta acggtgatgt gcaggttgaa
    34861 acgttttctt cgttgaagaa acaaggcgtg gacaagctgc ggcagaaact ggatacctgg
    34921 tttagcgaga tgcagcctgt agaagaaacg caggacggcg aataattttc ttgccttaat
    34981 gcttgtgccg gatgtggcgt atccggcccg taaattcaat agattacaat tctaccgttg
    35041 gcctgataaa catagcgcat caggcattac ggatcttttc tttcgcccaa taaaaaacgc
    35101 cccagtcatt actgactggg gcggctaaaa tattcagcca aatccgatta cgtgaagtaa
    35161 aaggtctgaa agatagaaca tcttacctct gtaccctacg cgactaactt tactcttttt
    35221 tgttcagttc agaaagcact ttttgtaatt ttttttcagt tgttgcatag gaatttctaa
    35281 tagccatcac aaaacgtgac agcttatgtt gcttacttac gaaaaaaggg catgttcagg
    35341 cgaatcttag tgcgcctgat cccagttttc gccactcccc acttccacca gcaacggcac
    35401 atccagacgg gtacagtttt ccatcagttg atgaatctgc ttcgcgacgg catcaacatc
    35461 atctttatga acttcaaata ccagttcatc gtgtacctgc atgatcatac gtacacgcgg
    35521 ttgctcagcc tgtaaccacg catcaacggc aatcatcgcc cgtttgataa tgtcggcggc
    35581 ggttccctgc attggcgcgt taatggctgc acgttcagcc gctgcacgac gagcaccatt
    35641 gctggatttg atatccggca gatacagacg gcgtccgtcc agcgtttcaa cgtagccctg
    35701 ctctttcgcc tgagcacggg tgcgttccat atactccagc acgccagggt agcgttcgaa
    35761 gtaaaggtcc atgtacttct gcgcttcttt acgtggaatg ttcaattgcc gcgccagacc
    35821 gaaagcactc atgccataaa tcagaccaaa gttgatcgct ttcgcgctac ggcgttgctc
    35881 gctggtgacg gtttccagtg gcaaaccaaa cacttctgcc gccgttgccc ggtggatatc
    35941 ttttccttcc gcgaatgcgg tcagcaagcc tttgtcacgc gagagatgcg ccataatgcg
    36001 cagttcaatc tgcgagtagt ccgctgagac aatcacataa tcctctggcg caataaacgc
    36061 ctggcggata cgacgacctt cttcgttacg caccggaatg ttttgcaggt taggatcggt
    36121 tgacgataaa cgtcccgttg cagttactgc ctggtgataa gaggtatgca cacgcccggt
    36181 tttcgggttg atcatcagcg gcagcttgtc ggtgtaggtc gatttcagct tcgccagacc
    36241 acgatactcc agaatcactt ttggcaacgg atagtccagc gccagttctt ccagtacctc
    36301 ttccgacgtt gacggcgcgc cacccggcgt tttcttcagc ggtttaatac cctgtttttc
    36361 aaacagaatg gtttgcaact gcttggtgga agaaaggtta aattcttcac ctgcaatttc
    36421 atgcgctttc ttttccagct cagccagacg caaggtgagc tcttcagaat gattgtgcag
    36481 cactttcgga tcgatcttca caccgttacg ttcaatgcgt gaaagcaccg gcaccagcgg
    36541 catttcgata ttctcgaaga cgttcaacgg ctctttgtgt ttttgcaggt ccggccacat
    36601 tttcagatgc aactgcaagg tgacatcagc atcttcggcg gcgtaacgtc cggcctcttc
    36661 gagggcaatc tggttaaagg tcagttgatt tttgccttta cccgcaatct cttcaaaagt
    36721 gatggttttg tgcttcaacc aacgttccgc gaggctgtcc atatcgtgac gcccggcaac
    36781 gctattgaga atgtaggact ccagcatggt atcaaacgca atcccacgca gctcaatgcc
    36841 gtaattcgcc agaataccgc gatcgtattt caggttttgc ccgaccttca gcgccttttc
    36901 atcttccagc agcggtttta gcaactcgag tgcacgctcg cgagagattt gatcgggcgc
    36961 atcaagataa tcatgagcaa ccggaatata tgccgctacg cctggctcga tagcaaaaga
    37021 aagcccgacc aggttagcag agatgttatc aaggctgtcg gtttcggtat caaatgcaaa
    37081 taccggcgct ttttccagct tcgcaatcca cgctttcagt gtttcttcat caaggatggt
    37141 gacgtagttg tcataagaaa tcaccgttgc cgtcacttct ggcgcttcgt ctgcaacact
    37201 ggtttcctgc ggcttcgcgg ctggttttgc ccctttggcc tgtaaccatt tgcccgcttc
    37261 gacatcagca gtccagcgtt tgaactcata ctttttgaac agccccaaca actcttccgc
    37321 tgccggttgc tgcacttcca gttgttcaca ggtcagctcc agttcaacgt cggttttaat
    37381 cgtcgccagc tggtatgaga gataagcaac ttctttgttt tgctcgagct tcgctgccat
    37441 tgttttcgcg ccacggaagc tcaacccagc aattttttct ggctcggcat acagcgtatc
    37501 cagcccgccc agaccttgca gcaatgcctg cgcggttttt tcaccgacgc ccggtacgcc
    37561 agggatgtta tcggaggagt cacccatcag cgccaggaaa tcgatgatca gttctggcgg
    37621 cacgccgtac ttattcacca cctcttccgg tccgaggatg gtattcgtca tggtattgat
    37681 aagcgtaata tttggcgtca ccagctgcgc catatcttta tcgccagtgc tgatcagcac
    37741 cggacgcccg gctttttcgg cttcgcgcgc cagagtaccg ataacgtcgt ccgcttctac
    37801 gccagaaacc gccagcagcg gcagtcccat cgctttaacc atcgcgtgca agggttcgat
    37861 ttgtgcacgc agatcgtccg gcattggcgg gcgatgtgat ttgtaatgtt caaacagttc
    37921 atcacgaaag gtttttccct tggcgtcaaa gaccaccgct gcatgcgtcg gtttatattg
    37981 catgatcaga ctgcgcagca tgttgaggac accatacatc gcaccggtcg gctcgcctgc
    38041 gctgttagtc agcgggggaa acgcgtgata tgcgcgataa agataagatg aaccatctac
    38101 aaggataagt ggattttggg ggatctgaac cataatgtcc gtgcctgttt atcagattat
    38161 gggtaaagga tgccacagac aggatgaaaa catgagtttt tcaccgcttt tcgtgaaaat
    38221 cttttgtaga tcttctggat cgctcgcaaa tcgtcatgtg gataactttg tgcataaatt
    38281 atttacatcg cttttaattt attatcatgc tttttacagc atgatgtttg tctttgtttt
    38341 tcatgcagtt aaaaataaat ccatccctaa taattgagat aatgggattt tttctaatgt
    38401 ggatagatat gaattatttt tctccttaag gatcatccgt tatttgggtc gttttctgct
    38461 aaaatctgcg ccttgcgctt ctttagcgca ctgtctatgg ctaacctttt gaataaattc
    38521 attatgacga gaatactcgc tgcgataacc cttttgctga gtatcgtatt gaccattttg
    38581 gtcactattt tttgttccgt cccgatcatc attgccggga ttgtaaaact attgctacct
    38641 gtgccagtca tttggcgaaa ggtttcgcgt ttttgtgatt ttatgatgta ttgctggtgt
    38701 gaaggtctgg cggtattact gcatctgaac ccacacctgc aatgggaagt tcacgggctg
    38761 gaagggttaa gtaagaagaa ctggtatctg cttatttgta accaccgtag ttgggcagat
    38821 attgtcgtac tgtgcgtgct gtttcgtaag cacattccga tgaataaata tttcctcaag
    38881 caacagctgg cctgggtgcc atttcttggc ctggcgtgct ggtcgctgga tatgccgttt
    38941 atgaagcgtt attcccgcgc ttatttatta cgtcatccgg agcgacgtgg taaagatgtt
    39001 gaaaccactc ggcgttcttg tgaaaagttt cgcctgcatc ccaccaccat tgtcaatttc
    39061 gttgaaggct cccgtttcac gcaagaaaaa catcagcaaa cccactccac ttttcaaaac
    39121 ttgttgccac caaaggcggc aggcattgcg atggcgctaa atgtactggg caaacaattc
    39181 gataaactgc ttaatgttac actgtgttat ccggacaata accgtcagcc gttcttcgat
    39241 atgttaagcg gtaaactgac gcggattgtc gtacatgtgg atttacagcc tattgccgat
    39301 gagttacatg gcgactacat caatgacaaa agctttaagc gccattttca gcaatggctg
    39361 aactcattgt ggcaggaaaa agaccggctt ctgacgtctc tcatgtcatc acagcgtcag
    39421 aataagtaag ttttactcgc ctctgaatga gcagaggcga gtgagtattt taatgaacaa
    39481 aacgtccggc acgagacata aattcttctt cgctcatctc ctgtccgtta aaaacaactt
    39541 tacccggtgt ataacgcagc tgcagcgagg cggtattgtt gtcaatcgtg attaactgaa
    39601 acatctgacc caatgtcatc atcccgctga tttgtttatc agcctgcttt tgcgcttttt
    39661 ccgcatccat tccttcagat aaattaagct gttttgcggt ttctgtgacg acattaagcg
    39721 gtaacttcac atcaaaattg agcgatttga tatctttgtt tgtggatgat gaagatttgg
    39781 ctgggtcggc aatactgata tccagattgg catttagttc gccgagtgcg ttcttccatc
    39841 ttaccggttg tttaatggtc ggctcacttt tttgtaataa cggtaaatac tctttgaaca
    39901 atgcagcatt cacttcttgc agagcaactt catcgtttgc taactcaggg tgtacagcaa
    39961 gctgcttctg catagcaatg ttatactgga tgataaattg gcgcactgct gagggatcaa
    40021 tagattcagc aatcaatgac cactcaccac taccgagttg ctgattccct tttgttaact
    40081 tatcaagttc gtaggtcaga ttgacattga tataatcttt gtctttaacg cgatccagca
    40141 tggtttttgc aacgaaagca tcgatttggg caacgtcttc cccgtgattg ataatgttaa
    40201 tctgattaaa tttcgactcg ctttcaccaa ccggaaattt cttttcttct aatcgcgcca
    40261 gagaatcaat ggtaaaactt tttgccgtta ctttgttcat tggtgataat tgcagaacaa
    40321 tatcttcgac tttgccttca atatccaggt tagacaatga actaccatca ccattaaaag
    40381 taaactggcc ctcaccaaaa gaaaactttc catactctgc aggggcaaca tctaatgtcg
    40441 tagagaatga gccaccaaac gcgaatcgca atgtcgcttc cacgggcgat ttattttttg
    40501 cggcgataaa tagcggttgt gtcagttcat tattcactaa gttcactttt gccgctgcca
    40561 gtgctgggat gacattacca tgcattaaca ttgtgatggg caacggaccg tgctcaacat
    40621 ccacatcaaa tacaactttc tggcctggct tgatattgag atcgggtgca ccgttatcga
    40681 atgtcatttg catttgaaaa cgggaattaa aaaagccttt gtcaaaattt tgataactta
    40741 acgtcatatc gtaggcatgt tcacctttct ttttagcatc gttaaaatct ttaataaatt
    40801 tttcgacacc aggctgaatt tgtgtacctg tgtaccatgt gccaccaccc cagattacgg
    40861 ctaacgcaac aataacacct gtagctgact tacgtatcat ataaatattc cctgataaaa
    40921 cagcaaaact ttgaatatcc acttatgctg aaataatgtc cagcggcagg atgcattatc
    40981 agaatgtatt tatatttatt aaa
//
LOCUS       DAZFQD010000029        40305 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.029, whole genome shotgun sequence.
ACCESSION   DAZFQD010000029 DAZFQD010000000
VERSION     DAZFQD010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 40305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40305
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.029"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(166..878)
                     /gene="lpxT"
                     /locus_tag="ACHKTW_001602"
                     /pseudo
     CDS             complement(166..878)
                     /gene="lpxT"
                     /locus_tag="ACHKTW_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311093.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
     gene            complement(917..1903)
                     /gene="yeiR"
                     /locus_tag="ACHKTW_001603"
     CDS             complement(917..1903)
                     /gene="yeiR"
                     /locus_tag="ACHKTW_001603"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HFO6462652.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSTLLKLRLATTA"
     gene            complement(2021..3487)
                     /gene="yeiQ"
                     /locus_tag="ACHKTW_001604"
     CDS             complement(2021..3487)
                     /gene="yeiQ"
                     /locus_tag="ACHKTW_001604"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFO6462653.1"
                     /translation="MKTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQVIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERGLTPFTVLSCDNIPDNGHVVKNAVLGMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFVAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFAHISDCMQDRAFRHAARTLMLNE
                     QAPTLQIKDVDLTQYADKLIARFANPALKHKTWQIAMDGSQKLPQRMLAGIRIHLGCE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIVQFGAHQALLNTLKI"
     gene            complement(3710..4282)
                     /gene="yeiP"
                     /locus_tag="ACHKTW_001605"
     CDS             complement(3710..4282)
                     /gene="yeiP"
                     /locus_tag="ACHKTW_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA];
                     GO_process: GO:0043043 - peptide biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HFO6462654.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLVKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            4437..4691
                     /gene="yeiW"
                     /locus_tag="ACHKTW_001606"
     CDS             4437..4691
                     /gene="yeiW"
                     /locus_tag="ACHKTW_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HFO6462655.1"
                     /translation="MKCRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            complement(4688..5869)
                     /gene="setB"
                     /locus_tag="ACHKTW_001607"
     CDS             complement(4688..5869)
                     /gene="setB"
                     /locus_tag="ACHKTW_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HFO6462656.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTIEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPVILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATLFCLLRIKD
                     V"
     gene            6237..7367
                     /gene="fruB"
                     /locus_tag="ACHKTW_001608"
     CDS             6237..7367
                     /gene="fruB"
                     /locus_tag="ACHKTW_001608"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HFO6462657.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDEHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEAGAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            7367..8305
                     /gene="fruK"
                     /locus_tag="ACHKTW_001609"
     CDS             7367..8305
                     /gene="fruK"
                     /locus_tag="ACHKTW_001609"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFO6462658.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            8322..10013
                     /gene="fruA"
                     /locus_tag="ACHKTW_001610"
     CDS             8322..10013
                     /gene="fruA"
                     /locus_tag="ACHKTW_001610"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HFO6462659.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWQGDISRAVAHPELFLSEAKGHAKPYTAPVAATAPVDASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTAQELDKAVAEATPYEPAGKAQTATTE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISECAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPETQIVEKNA"
     gene            10468..11409
                     /gene="psuK"
                     /locus_tag="ACHKTW_001611"
     CDS             10468..11409
                     /gene="psuK"
                     /locus_tag="ACHKTW_001611"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HFO6462660.1"
                     /translation="MREKDYIVIIGSANIDVAGYSHHPLNYADSNPGKIKFTPGGVGR
                     NIAHNLALLGKNAWLLSAVGGDFYGQSLLAQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMSISDCISAEFLAQHQEFIRGAKVIVADCNLSEEALVWVLENSGETP
                     VFIDPVSAWKCVKIRDHLSKIHTLKPNRLEAETLSGIALSGREDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDINGESGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFIDSV
                     RFAQGCSSMALACEYTNNPELSIANVRSLVENTECLN"
     gene            11397..12335
                     /gene="psuG"
                     /locus_tag="ACHKTW_001612"
     CDS             11397..12335
                     /gene="psuG"
                     /locus_tag="ACHKTW_001612"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HFO6462661.1"
                     /translation="MSELTLSPELLQISAEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEEIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSPFEVSIRLDSAREIARA
                     MAVKWQTGLNGGLVVANPIPEQFAMPEETINAAIDQAVAEAEEQGVIGKESTPFLLAR
                     VAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLVG"
     gene            12429..13679
                     /gene="psuT"
                     /locus_tag="ACHKTW_001613"
     CDS             12429..13679
                     /gene="psuT"
                     /locus_tag="ACHKTW_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HFO6462662.1"
                     /translation="MDIMRSVVGMVVLLAIAFVLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRMNRNELFTAICSGMASIAGSMMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSIIEAAANGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFANVSLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTSGTLDVKTIAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(13750..14448)
                     /gene="yeiL"
                     /locus_tag="ACHKTW_001614"
     CDS             complement(13750..14448)
                     /gene="yeiL"
                     /locus_tag="ACHKTW_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
                     /protein_id="HFO6462663.1"
                     /translation="MKEIHNNDLKQQLMSESAFKDCFLTDVSADTRLFHFLARDYIVQ
                     EGQQPSWLFYLTRGRARLYATLANGRVSLIDFFAAPCFIGEIELIDKDHEPRAVQAIE
                     ECWCLALPMKHYRPLLLNDTLFLRKLCVTLSHKNYRNIVSLTQNQSFPLVNRLAAFIL
                     LSQEGDLYHEKHTQAAEYLGVSYRHLLYVLAQFIHDGLLTKSKKGYLIKNRKQLSGLA
                     LEMDPENKFSGMMQ"
     gene            14578..15519
                     /gene="rihB"
                     /locus_tag="ACHKTW_001615"
     CDS             14578..15519
                     /gene="rihB"
                     /locus_tag="ACHKTW_001615"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HFO6462664.1"
                     /translation="MEKRKIILDCDPGHDDAIAMMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRKQIVADNIHGETGLDGPVFEPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERAGGPAGELFSD
                     IMNFTLKTQFENYGLAGGPVHDATCIGYLINPDGIKTQEMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            15619..16869
                     /gene="nupX"
                     /locus_tag="ACHKTW_001616"
     CDS             15619..16869
                     /gene="nupX"
                     /locus_tag="ACHKTW_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HFO6462665.1"
                     /translation="MDVMRSVLGMVVLLTIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEAAATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFAHASLESILGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTGGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(16925..18013)
                     /locus_tag="ACHKTW_001617"
     CDS             complement(16925..18013)
                     /locus_tag="ACHKTW_001617"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFO6462666.1"
                     /translation="MNDREKQILKILRRNPLIQQNEIADILQISRSRVAAHIMDLMRK
                     GLIKGKGYILTEQDYCVVVGAINMDIRGMADIRYPQAASHPGSVHCSAGGVGRNIAHN
                     LALLGRDVHLISAIGNDFYGETLLEETRRAGVNVSNCIRLHGHSTATYLAIANKQEET
                     ILAINDTHILQQLTPQLLNTSRDLIRHAGVVLADCNLTPEALEWVFTIADEIPMFVDT
                     VSEFKANKVKNWYSRIHTLKPTQNELEILWGQPIKDDNDRIRAVNSLHQQGVKRIFVY
                     LKDESVFCSDKDGEQFLLTAPAHTTVDSFGADDGFMAGLVYSFLEGYSFRDSARFAVA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(18017..18874)
                     /gene="nfo"
                     /locus_tag="ACHKTW_001618"
     CDS             complement(18017..18874)
                     /gene="nfo"
                     /locus_tag="ACHKTW_001618"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HFO6462667.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQDDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            complement(18948..19997)
                     /gene="yeiH"
                     /locus_tag="ACHKTW_001619"
     CDS             complement(18948..19997)
                     /gene="yeiH"
                     /locus_tag="ACHKTW_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HFO6462668.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAVGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            20096..20977
                     /gene="yieE"
                     /locus_tag="ACHKTW_001620"
     CDS             20096..20977
                     /gene="yieE"
                     /locus_tag="ACHKTW_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YeiE"
                     /protein_id="HFO6462669.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTREIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            21182..22651
                     /gene="lysP"
                     /locus_tag="ACHKTW_001621"
     CDS             21182..22651
                     /gene="lysP"
                     /locus_tag="ACHKTW_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HFO6462670.1"
                     /translation="MVSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            22944..24930
                     /gene="cirA"
                     /locus_tag="ACHKTW_001622"
                     /pseudo
     CDS             22944..24930
                     /gene="cirA"
                     /locus_tag="ACHKTW_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
     gene            complement(24962..25798)
                     /gene="yeiG"
                     /locus_tag="ACHKTW_001623"
     CDS             complement(24962..25798)
                     /gene="yeiG"
                     /locus_tag="ACHKTW_001623"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HFO6462671.1"
                     /translation="MEMLEEHRCFEGWQQRWRHDSSTLNCPMTFSIFLPPPRDHTPPP
                     VLYWLSGLTCNDENFTTKAGAQRVAAELGIVLVMPDTSPRGEKVANDDGYDLGQGAGF
                     YLNATQPPWATHYRMYDYLRDELPALVQSQFNVSDRCAISGHSMGGHGALIMALKNPG
                     KYTSVSAFAPIVNPCSVPWGIKAFSSYLGEDKNAWLEWDSCALMYASNAQDAIPTLID
                     QGDNDQFLADQLQPAVLAEAARQKAWPMTLRIQPGYDHSYYFIASFIEDHLRFHAQYL
                     LK"
     gene            26056..26724
                     /gene="folE"
                     /locus_tag="ACHKTW_001624"
     CDS             26056..26724
                     /gene="folE"
                     /locus_tag="ACHKTW_001624"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HFO6462672.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            26741..27898
                     /gene="yeiB"
                     /locus_tag="ACHKTW_001625"
     CDS             26741..27898
                     /gene="yeiB"
                     /locus_tag="ACHKTW_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 domain-containing protein YeiB"
                     /protein_id="HFO6462673.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYGAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLICWRLVRDAPSVKSLFNTGVMLYLVGLGVLLLLGLISDSQTSRA
                     WTPDASAILYEKYWKLHGGVDAISNRADGVGNSLLALGAQYGWQLAGMMLIGAALMRS
                     GWLKGQFSLRHYRRTGFVLVAIGVTINLPAIALQWQLDWAYRWCAFLLQMPRELSAPF
                     QAIGYASLFYGFWPQLSRFKLVLAIACVGRMALTNYLLQTLICTTLFYHLGLFMHFDR
                     LELLAFVIPVWLANILFSVIWLRYFRQGPVEWLWRQLTLRAAGTAISKTSR"
     gene            28040..29080
                     /gene="galS"
                     /locus_tag="ACHKTW_001626"
     CDS             28040..29080
                     /gene="galS"
                     /locus_tag="ACHKTW_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HFO6462674.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAVFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMTKLATELALQGAAGNIDPRASHCFMPTLVRRHSVATRQN
                     AAAITNSTNQAM"
     gene            29360..30358
                     /gene="mglB"
                     /locus_tag="ACHKTW_001627"
     CDS             29360..30358
                     /gene="mglB"
                     /locus_tag="ACHKTW_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HFO6462675.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            30419..31939
                     /gene="mglA"
                     /locus_tag="ACHKTW_001628"
     CDS             30419..31939
                     /gene="mglA"
                     /locus_tag="ACHKTW_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043211 - ABC-type carbohydrate
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HFO6462676.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKQINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            31955..32965
                     /gene="mglC"
                     /locus_tag="ACHKTW_001629"
     CDS             31955..32965
                     /gene="mglC"
                     /locus_tag="ACHKTW_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HFO6462677.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKVSGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(33036..34271)
                     /gene="preA"
                     /locus_tag="ACHKTW_001630"
     CDS             complement(33036..34271)
                     /gene="preA"
                     /locus_tag="ACHKTW_001630"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017113 - dihydropyrimidine dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0006210 - thymine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HFO6462678.1"
                     /translation="MLTKDLSITFCGVKFPNPFCLSSSPVGNCYEMCAKAYDTGWGGV
                     VFKTIGFFIANEVSPRFDHLVKEDTGFIGFKNMEQIAEHPLEENLAALRQLKEDYPDK
                     VLIASIMGENEQQWEELARLVQEAGADMIECNFSCPQMTSHAMGSDVGQSPELVEKYC
                     RAVKRGSTLPMLAKMTPNIGDMCEVALAAKRGGADGIAAINTVKSITNIDLNQKIGMP
                     IVNGKSSISGYSGKAVKPIALRFIQQMRTHPELRDFPISGIGGIETWEDAAEFLLLGA
                     ATLQVTTGIMQYGYRIVEDMASGLSHYLADQGFDSLQEMVGLANNNIVPAEDLDRSYI
                     VYPRINLDKCVGCGRCYISCYDGGHQAMEWSEKTRTPHCNTEKCVGCLLCGHVCPVGC
                     IELGEVKFKKGEKEHPVTL"
     gene            complement(34265..35503)
                     /gene="preT"
                     /locus_tag="ACHKTW_001631"
     CDS             complement(34265..35503)
                     /gene="preT"
                     /locus_tag="ACHKTW_001631"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
                     /protein_id="HFO6462679.1"
                     /translation="MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDP
                     GKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEKLCQSGCTRAGVDTPIDIGRLQ
                     RFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAHPG
                     GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEVGNTLTLEQLKAENRAVLVTVG
                     LSSGSGLSLFEHSDVEIAVDFLQCARQAQGDISIPQSALIIGGGDVAMDVASTLKVLG
                     CQAVTCVAREELDEFPASEKEFASARELGVSIIDGFTPVAVEGNKVTFKHVRLPGELT
                     MAADKIILAVGQHARLDAFAELEPQRNTIKTQNYQTRDPQVFAAGDIVEGDKTVVYAV
                     KTGKEAAEAIHHYLEGACSC"
     gene            complement(35822..36061)
                     /gene="yeiS"
                     /locus_tag="ACHKTW_001632"
     CDS             complement(35822..36061)
                     /gene="yeiS"
                     /locus_tag="ACHKTW_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HFO6462680.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            complement(36064..36783)
                     /gene="sanA"
                     /locus_tag="ACHKTW_001633"
     CDS             complement(36064..36783)
                     /gene="sanA"
                     /locus_tag="ACHKTW_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HFO6462681.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            complement(36933..37817)
                     /gene="cdd"
                     /locus_tag="ACHKTW_001634"
     CDS             complement(36933..37817)
                     /gene="cdd"
                     /locus_tag="ACHKTW_001634"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFO6462682.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            complement(37947..38642)
                     /gene="yohK"
                     /locus_tag="ACHKTW_001635"
     CDS             complement(37947..38642)
                     /gene="yohK"
                     /locus_tag="ACHKTW_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HFO6462683.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            complement(38639..39037)
                     /gene="yohJ"
                     /locus_tag="ACHKTW_001636"
     CDS             complement(38639..39037)
                     /gene="yohJ"
                     /locus_tag="ACHKTW_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFO6462684.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAI
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            39276..40223
                     /gene="dusC"
                     /locus_tag="ACHKTW_001637"
     CDS             39276..40223
                     /gene="dusC"
                     /locus_tag="ACHKTW_001637"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HFO6462685.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGSWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCMAISGC
                     DAVMIGRRALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVRVLNNSPDIARAIQAIDIEKL"
BASE COUNT         9958 a         9836 c        10528 g         9983 t
ORIGIN      
        1 gttaataagc gggattggtc aattaaagta cgattgagtg aaacgcatgc aatccattca
       61 tttacttaga gaatgatttt tgcgttcgat agatgaaagc acggtgcgca tcatcaggat
      121 tatcctcact ataaaaataa acctgatgat gttaattact gtgagttatt tgttttggaa
      181 atgtttgttt tttcccggta gtgatttgtc aaaaaaggtg attaatcgat cacttaatgg
      241 cgtcagcaac acccagggca aaccgatcaa tatcacggtc atcgaaccga caatgatgtc
      301 agtaaaccag tgtgcgccaa tcattactct gggaaacgca aaaaccacaa aaataataag
      361 ggcgataaga cctgtaactt gccgaaataa cgccacatga atgccgaaaa aataagcagc
      421 atcatgccgt gatcgccggg aaagctatct cgtgaggcat ctttcgtggg aacagagagc
      481 agttcgctga cgcggttaat gtcggtaaaa gtcaatgttg ggctggcccg ttttacagga
      541 attaatgcct gacccagctg gtttaatacc actgcagtta atagcatgac cagacccata
      601 atcacgatac gtcgtctgcc tggggcgttt tctttcagcc agaaactcag catcaacata
      661 cccatcgcca gcagtgaaca accgtcgaag gcgcgattgt tggtcaatgc aaccagccac
      721 aaaaaggcct tactttcgac cagtttctga ttaaagaaat aaaaaatatc cgcatcaatc
      781 ggcaaccaga atccatgatt aacgggaata taccaggaaa gaaacagcgc gaggccgaca
      841 atattcaaca acactatttg cggcaaattt ttaatcatat tttctctaat tatcttactg
      901 aaaaacgcag gcaaccttac gcggtagtcg ctaaacgaag cttcaacagc gtgctctgca
      961 aagcattcca gtcagcttcg ctgctggaaa tcagctcaat acgactgtcc ggtggcgcaa
     1021 cgttttgcgt ttcaatgtgc aggtcatcgc cctgacggtt gattcgcacc agcccttcgg
     1081 gaatacgcag cacgcctttg acgcgttcca ccggtgcaag tcgcgcccat tccagaatgc
     1141 caatggtgtc gaataccgta tcagcgtcga atatccagcc gcaggcctga tatccttgcc
     1201 cgctgttcag actgcgacgc cagcgttgat gctctggcag gcttaacgct gctaaccctt
     1261 ttttcacgac atgctgatga gaatgcgcgg cgctggcggg caactcggct aaattgcgac
     1321 gcggcaaatc cagaagatga ccgtcaactt tcccatgctc actgtgaatt aattgtcgat
     1381 cgccaccatt ttgctgccac caacgctgta gcgcttgctc actttcgggc gtcgcacggt
     1441 cggatttatt ggcgacaatg atgtctgcgg cagccagctg gtcacggaag ttttcattgc
     1501 tggcgctttt ttcgtccagc agcaggcgtg gatcgagaat gcacaaggtg gcgcgcagat
     1561 ctatccacgg ttcatagact ggtgcggtta acagatcgag gatctgtttc ggatggccca
     1621 gcccggtcgg ctctatcaac aagcggtctg gttttccctg acgcagtaag gtattcaacc
     1681 ctacctgcat gggtaaacca ttaacgcagc acatgcagcc gccggggatc tctttcagca
     1741 atgcgccgct atcggcgagc aaagcaccat caattccgac ttccccaaat tcattaacca
     1801 gtaccgccca tttttcgttg ggatctttat gggctaacag atgaagaatc gacgtggttt
     1861 tcccgctgcc gagaaaaccg gtgatgaggt tggtcctggt cacaacggct ccgcaatact
     1921 ctggtaagtt aactgagcgt taatcctggc taaaaaaggc agaaaagaga aggggagagc
     1981 ggacgaaggt gagcttcatc cgctttaacc gcagaaatcg ttaaattttg agggtgttta
     2041 atagcgcctg atgtgcgccg aattgtacga tttgttgcca ggcttgttcg atggcctgca
     2101 caaaatgcgg gttatctggc agatcatcac cgaaaacttc acgcagggaa agcagggcgg
     2161 ttacgcgttg ttcactgctg ctgcccgcaa caagttcgcg aattttatcg ctaagcggat
     2221 cgcgaacatc aatggcattt ccggcatcat caacgccgct gacgtaacgc atccagccag
     2281 caacgcctaa tgccagcaac gaccagtccg tttcgcaccc cagatgtatg cgaatacctg
     2341 ccagcatacg ttgcggtaat ttctggctac catccattgc gatttgccag gtcttatgtt
     2401 tcagcgccgg attagcaaaa cgtgcaatta acttgtccgc atattgtgtt aaatcgacat
     2461 ctttaatttg tagtgtcggc gcttgctcat tcagcattaa tgttctggcg gcatggcgaa
     2521 atgcgcgatc ctgcatgcaa tcactgatat gggcgaatcc tgaaaggtaa cccagataag
     2581 cgagaaaaga gtggctgcca ttaagcatac gcagtttcat ctcttcccat ggcaggacat
     2641 cattcaccat ttgtacacct gcgacttccc aggcaggacg cccagcgacg aagttatctt
     2701 ccaccaccca ctggataaac ggttcgcagc taatcgcgca gggatcattt acccccagat
     2761 gctggctgat ttctgccagt gattcgtcgg ttgcagccgg aacaatgcgg tcgaccatgg
     2821 ttcccggaaa actgacgtgt tcttttatcc acccggcgag ttctggcgaa cgtttttctg
     2881 ccattcccag caccgcgttt ttcaccacat ggccattgtc gggaatattg tcgcaggaga
     2941 gcacggtaaa cggtgtaagg ccgcgctcac ggcggcgttt cagtgcttcg acgagaatac
     3001 ccggtgcgga gtgaggttct tcaggggttt gtagatcgtg aataatccgc ggattactgg
     3061 tgtcgagtgc accggtggcc gggtcaatac aatagccttt ttcggtaatc gtcagggaaa
     3121 caattgccac ctgtggctcg caaaatttct caataattgc cgctaaggaa tccagttttg
     3181 cattaaggca ttcgtgaacg gcaccgacaa ttatcacctg attgccgtcc gcaccttttt
     3241 ccagcacggt atataaatgg ttctgtgcgc ggagctggct catcagttga tcaccgctga
     3301 acaagctgat ttcacagatc ccccagtcgc cgccctgggc attcagcaca cgatcggtca
     3361 gtaacgcctg atgagcgcgg tgaaaggcgc caaagccaaa atgaacgata cgtgattgca
     3421 actgctggcg atcgtagcgt ggagcgtgta catgatgcgg gagcgtaacg gaggcaattg
     3481 tcttcatagg agtcacgtct tagtaacgat aaattaacaa tgacagtgta aagttatatg
     3541 acagcaattg aaattggtct ggattgttaa tgcgatgaag tgtgagggcg atcaatcaac
     3601 gagtgaaaat tgagggcgta aggtgttcag gaagcgcaaa atagaagatt ggtataataa
     3661 attaaaaaag ggacagcctg agctatccct tttctgcatg cggctgaagt tagtcagcac
     3721 gccccatata acggcgttct tcgatatgga tacgaatttt ttcgcccggg cttaagtatt
     3781 ctggtacctg aatcaccaga ccagtgctca atgtcgccgg tttgttacgg gcgctggcag
     3841 atgccccttt gatgcctggt gcggtttcaa cgatttccag atcaacggtc tgcggcagct
     3901 caagcgccag cagttgaccg tcccaggtca gcacctgcat gtccggcatg ccgccctctg
     3961 gcataaacag caactcttct tcaatctgat ctttggtgaa ggtatacggg gtatagtctt
     4021 ctttatccat aaagacatat tcgttgccat cgacatagga gaagtcaacg taacggcggg
     4081 tcagcgtcac ggtgtcaacg atatcatcac ctttgaaacg ctcttctact ttcagcccgg
     4141 tacggacatc agaaaaacgc attttgtaca gcgtagcggc accgcgggca gtgggcgact
     4201 gaatatcaat atccttaacc agcagcagtt tgccgttgta attcagtacc ataccttttt
     4261 taatttcgtt cgctcttggc atcgaaaaat tcctgtagtc gaaaatgtca aaaatatcgc
     4321 gacaaagtac tcgcgacggg cctttcgggc aagtggtatt cgcacttttg ctggtgcaaa
     4381 aaaggtggta ctgtgcgcgc tcatcaatcc ggtggttaac cttaagagaa caacgtatga
     4441 aatgccgtcc gggttgcggg gcgtgttgca ctgccccttc aatttccagc cctattcccg
     4501 gtatgccaga tggcaagccc gccaatacgc cctgcattca gcttgatgaa cagcaacgct
     4561 gtaaaatttt cacctcgcca ctgcgaccga aagtctgtgc aggcttacaa gccagcgctg
     4621 aaatgtgcgg taactcgcgt cagcaggcca tgacctggct tatcgatctg gaaatgctga
     4681 ccgcgcctta aacatcttta atccgcagta agcaaaacag agtggcgata atcatcacca
     4741 tcgcaaacca gaacacagcg tgataattcc agatctcggc gacgatgccc gccactgatc
     4801 ctgcgatgat ccagcccacg cgcgacgtgt tggtatagag cgtggtggct gaacccgcct
     4861 gaccgggcat cagatcctga aaatagagca tcccgatgcc gcccagaatg ccaataaaaa
     4921 tagcatttag cagctgcaag cccaacagaa tgacaggtga atgcgccatc agcattcctg
     4981 cgtaaaaaca gacgccaccc acggcagcaa cgcgcattaa gaaacgctta cccagacgtt
     5041 tggcgaaata tccggcaatc aacatcgtcg ggatttccag cccggcggcg gtccccatca
     5101 tcacaccggc cagtttctcg ggaagatgca gttcgttgat aataaatagc ggcatgttga
     5161 tgatgtacag gctgttcgag ccccacatca atgtacaaat gacaaacagc agcagcgtat
     5221 cgcgacggtt acggcgcggc gcttcgatcg tgccggtcgc cagcggaagc tctttttgca
     5281 tcgacggtaa aaacagccac accatcacac cgcaaacaat aaacgctacc gctgcgctca
     5341 gatacattac cgtaaagctg aaacccatcg ctaaggcata agccagcggt gggccaatga
     5401 cccatgccag tgaaacctga gcgcgtaaaa aagagctgaa catcaccgcc tcacgtccgg
     5461 ttttgtcggc atgttcacgg gcaagggcaa acatttgcgg gttagcggtc gagccaaagc
     5521 tgctaagaaa gacgccaacg aatagcaaaa caaagtagtt gcgattccag gcaaaaaggg
     5581 tgcaggccag cacgcctaac aggcagcaaa agacaatcag cgatttgcga tcgccgcgct
     5641 tatcagagcg cccggcgaga aactgactta ccagaatccc aatgacagcg ctgccggtaa
     5701 agaagaatcc caccatcgcc ggacgagcat gtacttcatc agtaagaaaa atactgagtg
     5761 tcggggtttg cagagcgccc gcaataccgg tgagaaaggc aacgattaaa aacgccgtcg
     5821 aggtcaggtc aaacgatttc gcgctggaga ctgcggggga gttatgcatg ctggtcgatc
     5881 actggaagag gaagacgcgg agtttacgct gcttgtcaga aaataaacag acagcgaaat
     5941 caaaagcgcc acgaataatc aaaatgccat ttcactgttg ctgattggct gctgacgctg
     6001 ctgaaatgaa attgctgata acggattttc ccatcagcaa ttaggaaaaa tggcaaaaaa
     6061 ttgtgcagca catcaaactt ttgctcataa ctttacggct ttccttgcgt gctgaaacag
     6121 aagtattatg ctttcttgaa acgtttcagc gcgatcttgt ctttaaccct aagccaggtt
     6181 ggcgcttttt ttctcataga ggctgaatcg tttcaattca gcaagagagg agaactatgt
     6241 tccagttatc cgtacaggac atccatccgg gcgaaaaggc cggagacaaa gaagaggcga
     6301 ttcgccaggt cgctgcggcg ctggtgcagg ccggtaatgt agcagaaggc tacgtcaatg
     6361 gcatgctggc gcgcgaacag caaacctcaa cgttcctcgg caatggtatt gctattccac
     6421 acggcactac cgacacccgc gatcaggtgc tgaaaaccgg cgttcaggta tttcagttcc
     6481 cggaaggcgt cacctggggt gacggtcagg tagcgtacgt ggcaatcggt attgctgcca
     6541 gctcggatga acatctgggc ctgctacgcc agctgaccca cgtactgagc gatgattccg
     6601 ttgctgaaca actgaagtca gcaacaacag cagaagaact tcgcgcatta ctgatgggcg
     6661 aaaagcagag tgagcagctg aagctcgaca acgaaatgct gacgctggat atcgtcgcca
     6721 gcgatctgct gactcttcag gcgctgaacg ctgcgcgtct gaaagaggcg ggggcagttg
     6781 acgccacttt cgtcaccaaa gccatcaatg aacaaccgct gaacctcgga cagggtatct
     6841 ggctgagcga tagcgccgaa ggcaatctgc gtagcgcgat tgcggtaagc cgtgcggcaa
     6901 atgcttttga tgtggacggc gaaacggcag ccatgctggt gagtgtggcg atgaatgacg
     6961 atcagcccat cgcggttctt aagcgtctcg ctgatttgtt gctcgacaat aaagctgatc
     7021 gcttgctgaa agcggatgcg gcaacgttgc tggcgctgct gaccagcgat gatgcgccga
     7081 ccgacgacgt gttaagcgcg gagtttgtgg tgcgcaatga acacggcctg catgctcgtc
     7141 caggtaccat gctggtcaat accattaaac aatttaacag tgatattacc gtgacaaacc
     7201 ttgatggcac cggcaaaccg gcaaacggac gtagtctgat gaaagttgtg gcacttggcg
     7261 ttaagaaagg tcatcgccta cgctttaccg cccagggtgc agatgctgaa caggcgctga
     7321 aagccattgg cgacgctatc gctgctggtc ttggggaggg cgcataatga gcagacgtgt
     7381 tgctacgatc acccttaatc cggcttatga ccttgttggt ttctgcccgg aaattgaacg
     7441 cggcgaagtg aacctggtga aaaccaccgg tctgcatgcg gcgggtaaag gcatcaacgt
     7501 ggccaaagta ttaaaagacc tgggaattga tgtcaccgtt ggcggcttcc tcggtaaaga
     7561 caatcaggat ggttttcagc aactgttcag cgagctgggc attgccaacc gtttccaggt
     7621 tgtacagggg cgcacccgaa ttaacgttaa gctgacggaa aaagacggcg aagtgaccga
     7681 cttcaacttc tcgggttttg aagtcacccc cgccgactgg gaacgctttg tgactgattc
     7741 tctgagctgg ctcggtcagt tcgatatggt ctgtgtcagc ggaagcttac cgtcaggcgt
     7801 cagcccggaa gcgttcaccg actggatgac tcgcctgcgt agtcagtgtc cttgcattat
     7861 ctttgatagt agccgtgaag cgttagtagc aggtttgaaa gcggcaccgt ggctggtgaa
     7921 acctaaccgc cgcgagctgg aaatctgggc aggccgtaaa ctgcctgaaa tgaaagatgt
     7981 gattgaagct gcacatgcgc tacgtgaaca aggtattgcg catgttgtta tttcactggg
     8041 tgccgaaggc gcgctttggg ttaatgcctc cggcgaatgg atcgccaaac caccgtcagt
     8101 cgatgtcgta agcaccgttg gcgcagggga ttctatggtt ggtggcctga tttatggctt
     8161 gctgatgcgt gaatccagtg aacacacact gcgtctggcg actgctgttg cagccctggc
     8221 ggtaagtcaa agcaatgtgg gtattaccga tcgtccgcag ttggccgcaa tgatggcgcg
     8281 cgtcgactta caacctttta actgacagca ggagaggcat aatgaaaacg ctgctgatta
     8341 ttgacgctaa tctcggtcag gcacgcgcct atatggcgaa gaccctgctg ggcgcggcgg
     8401 cgcgaaaagc aaaactggaa atcatcgaca atccgaacga cgctgaaatg gcgattgttc
     8461 tcggtgattc catcccgaac gacagcgcgc tgaacggtaa aaatgtctgg cagggcgata
     8521 tttcccgggc agttgcgcac cctgagctgt tcctgagtga agccaaaggc catgcgaaac
     8581 cttacactgc gccggtcgct gcgacagcac cggttgacgc cagcggcccg aaacgcgtag
     8641 ttgcggtgac tgcttgcccg actggcgtag cacacacctt tatggctgct gaagccattg
     8701 aaaccgaagc gaaaaaacgt ggctggtggg tgaaagttga aacccgtggt tctgttggcg
     8761 cgggtaatgc aatcactccc gaagaagtag ccgcagcgga tctggtgatt gtggcggcag
     8821 atatcgaagt ggatctggcg aaatttgctg gtaaaccgat gtatcgcacc tctaccggtc
     8881 tggcgctgaa gaaaaccgcg caggaactgg ataaagcggt tgctgaagca acgccgtatg
     8941 aaccggcggg caaagctcaa acggcgacca ctgaaggtaa gaaagagagt gcgggcgctt
     9001 accgtcactt gctgacgggc gtttcttaca tgctgccgat ggtcgttgct ggtggtctgt
     9061 gtatcgcgct ttcttttgct tttggtatcg aagcgtttaa agagccgggt acgttggcag
     9121 cggcgctgat gcagattggc ggtggttcag cctttgcgct gatggtgccg gtactggcag
     9181 gttatattgc cttctccatt gccgatcgtc cgggcctcac gccgggtctg attggcggta
     9241 tgctggctgt cagcaccggt tctggcttca ttggcggtat tattgcgggc ttcctggctg
     9301 gttacattgc gaagttaatc agtacgcaac tgaaactgcc acagagtatg gaggcgctga
     9361 aaccaatcct gatcattccg ctaatttcca gtctggtggt cggtctggcg atgatctacc
     9421 tgatcggtaa accagttgct ggcattctcg aagggctgac tcactggctg cagaccatgg
     9481 ggactgcgaa tgcggttctg ctgggggcga tcctcggtgg catgatgtgt actgacatgg
     9541 gcggtccggt aaataaagca gcgtacgcgt tcggtgtggg tctgctgagt actcaaacct
     9601 atggcccgat ggcggcgatt atggcggcag gtatggtgcc accgctggca atgggtctgg
     9661 caacaatggt ggcgcgtcgc aaattcgaca aagcgcagca ggaaggtggc aaagccgctc
     9721 tggtattggg actgtgcttc atttcggaat gtgcaattcc gtttgctgct cgtgatccga
     9781 tgcgtgtgct gccgtgctgt atcgtgggtg gggcgctgac tggcgcaatc tcaatggcga
     9841 ttggtgcgaa actgatggca ccgcacggtg gtctgtttgt tctgctgatc cctggcgcga
     9901 ttacgccggt actgggttac ctggtagcca ttattgccgg tacgctggtg gcgggtctgg
     9961 cctatgcctt cctgaaacgc ccggaaacac aaatagtaga aaaaaacgca taatttatct
    10021 tcattatcgg ggatgagaca tctctgcccc gatgatgaca aacgcaaaat tgcctgatgc
    10081 tctatgctta tcagacctac atgaaccctg caatatattg aatttttgcg cttttgtagg
    10141 tcggataagg cgtttacgcc acatccagga tgaacaaagc gcgcgttgtc aacaatctgg
    10201 agggcagaaa catctctgcc ctttttcttg cctgccatat tgtgttacac atcacaactt
    10261 tctcttttgt tgctgcataa atagacaaac tttgtgaccg ccttcatatc tggccgattt
    10321 cgttttttct gatcgattta cattacgtta aatatgctta ttatgaacat gtgtttaaat
    10381 atgaacacgt gctctaatag tcttattgac tgtaatgact attttaccca atttcttcgt
    10441 tggcaattta ttgccagggg gcgtgctatg cgcgaaaagg actacatagt aattattggt
    10501 tcggcgaata ttgatgtggc tgggtattca catcatccat tgaattatgc ggattcgaat
    10561 cccggaaaaa ttaaatttac cccaggtggc gtgggaagga atatcgctca taatctggcg
    10621 ttactgggga aaaatgcctg gcttttaagt gcagtaggtg gtgattttta tgggcaatca
    10681 ttactggcgc aaactaatca atccggtgtt tatgtcgata aatgcttaat tgttccgggt
    10741 gagaatacat ccagctattt atcactgctc gataatacag gagaaatgct tgtcgcgata
    10801 aatgatatga gtattagtga ctgtatttct gcagaatttc ttgcccagca tcaggagttt
    10861 attcgcggtg caaaggttat tgtcgcggat tgcaacctta gtgaagaggc gctggtctgg
    10921 gttctggaaa atagcgggga aacgcctgta tttattgatc cggtatcggc atggaaatgc
    10981 gtcaaaatcc gcgatcatct gagcaaaatc catacgttaa aacctaaccg tcttgaagcc
    11041 gaaacgttga gcggaattgc actttccgga cgtgaagatg tcgcaaaggt cgccgcctgg
    11101 tttcatcaac acggcctgaa ccgcctggtg ctgagcatgg gaggggatgg cgtttactac
    11161 agtgatatca acggtgaaag tgggtggtcg gcccctatta aaacgaatgt catcaatgtt
    11221 actggggcag gcgacgccat gatggcgggg ctggcctcct gttgggtaga cggtatgccg
    11281 tttatcgatt ctgttcgatt tgcgcaggga tgttcgtcaa tggcacttgc ctgtgaatat
    11341 accaacaacc ctgaattatc aattgccaat gttagatcgt tagtggagaa cacagaatgt
    11401 ctgaattaac tctgtcccct gaattattac aaatatccgc tgaagtacag gatgctttaa
    11461 aaaataaaaa accggttgtt gcgctggaat cgaccataat ttctcacgga atgccattcc
    11521 cacaaaatgc ccaaaccgca attgaagtag aagaaactat tcgtaaacag ggcgcagtac
    11581 cagccactat cgccattatt ggcggtgtga tgaaagtggg cttaagtaaa gaagaaatcg
    11641 aattgctggg ccgtgaagga cataacgtga ccaaagttag tcgtcgcgat ttaccttttg
    11701 ttgttgccgc cggaaaaaat ggcgcaacca ctgtggcttc aacgatgatt attgcggcgc
    11761 ttgccggaat taaagtattt gccaccgggg gaattggtgg tgtgcatcgc ggggcggaac
    11821 ataccttcga tatttctgcc gatttacagg aactggcaaa taccaatgtc actgttgttt
    11881 gtgcgggggc aaaatctatt ctcgatttag gattaacaac agaatattta gaaaccttcg
    11941 gtgtaccgtt aattggttat cagacaaaag cgctgcctgc gtttttctgc cgaaccagcc
    12001 catttgaagt cagcattcgt cttgacagtg cccgcgaaat tgctcgcgcg atggcagtga
    12061 agtggcaaac cggtttgaac ggcggcctgg tagtggcgaa tccgatcccg gaacagtttg
    12121 cgatgccaga agaaaccatc aatgcagcaa ttgatcaagc cgttgctgaa gccgaagaac
    12181 agggtgttat tgggaaagag agtacaccgt tcctgctggc gcgtgttgcg gagctaacag
    12241 gcggtgatag cctgaaatcc aatattcaac tggtctttaa taacgccatt ctggcgagcg
    12301 aaattgccaa agaatatcag cgtctcgtgg gttaatttct ggcgggcaga aagccgtcgc
    12361 ccgtactcat caaacatacc aaaacctggc gctgacgccg ggtttactgg catgaaggga
    12421 aaatcattat ggatataatg agaagtgttg tggggatggt ggtattgctg gcaattgcct
    12481 ttgtgttgtc ggtaaataaa aagagcatca gcttgcgcac ggttggcgct gcgctgctgc
    12541 tgcaaattgc cattggtggc atcatgctct attttccccc gggaaaatgg gcagtagagc
    12601 aggcggcatt aggcgtccat aaagtgatgt cctacagtga cgcaggaagt gcctttattt
    12661 ttggttcgct ggtcggtccc aaaatggatg ttctgtttga cggtgcgggc tttatcttcg
    12721 cctttcgcgt actcccggcg attattttcg ttaccgcgct tatcagcttg ctgtactaca
    12781 ttggcgtaat ggggctgctg attcgtattc ttggcagtat ttttcagaaa gccctcaaca
    12841 tcagcaaaat cgaatctttt gtcgcagtca ccactatttt cctcgggcaa aatgagatcc
    12901 cggcgatcgt taaacctttt attgatcgca tgaatcgcaa cgagctattt acggctattt
    12961 gtagcggcat ggcgtctatt gcgggttcga tgatgattgg ctatgccgga atgggcgtgc
    13021 cgattgacta tttgttagcc gcttcgctga tggctattcc tggcggtatc ctgtttgcac
    13081 gtattcttag tccggcgacg gaaccttcgc aagttacgtt tgaaaatctg tcgtttagcg
    13141 aaacaccgcc aaaaagcatt attgaagctg cggcgaacgg tgcaatgacc gggctgaaaa
    13201 tcgccgccgg tgtggcgacg gtggtaatgg catttgtggc aattattgcg ctgatcaacg
    13261 gcattattgg tggagttggc ggctggtttg gttttgccaa tgtctctctg gaaagcattt
    13321 tcggctatgt gctggccccg ttggcatgga ttatgggggt ggactggagt gatgcaaatc
    13381 ttgccggaag tttgattggg cagaaactgg cgattaatga gtttgttgct tatctcaatt
    13441 tctcgcccta tctgcaaacg agcggcacgc tggatgtcaa gaccattgcc attatctcgt
    13501 tcgccctctg tgggtttgca aactttggtt ctatcggtgt tgtcgttggc gcattttcgg
    13561 ctatttcgcc aaaacgtgct ccagaaatcg cccagcttgg tttacgggcg ctggcagcag
    13621 caacgctttc caacctgatg agtgcgacta ttgccgggtt ctttattggt ctggcgtaat
    13681 gctcgatttg tagaccggat aaaacgtatt aagcgttgca tccggcaatc gcgctattgc
    13741 aaataatttt tactgcatca tcccggagaa tttattctcc gggtccatct ccagcgccag
    13801 tcctgacaac tgctttctgt ttttaatgag ataccctttc ttgcttttag ttaataaacc
    13861 gtcgtgaatg aattgcgcga gaacatataa aagatgtcga taagaaacgc ctaaatactc
    13921 tgccgcttgc gtatgctttt cgtgataaag atcaccttcc tgcgagagta atataaaagc
    13981 tgccaggcga ttaactaacg gaaatgattg attctgagtt aaagaaacaa tattacgata
    14041 atttttatga cttaaggtga cgcagagttt tcgtaaaaat agcgtgtcgt ttaataacag
    14101 cgggcggtaa tgtttcatag ggagcgcaag gcaccaacac tcttcaatag cctgcaccgc
    14161 acgcggttca tggtctttgt cgattaactc aatctcgcca ataaaacagg gggcggcaaa
    14221 gaaatcgatc agcgacacgc gaccattagc tagcgtggcg taaagcctgg cgcggcctcg
    14281 cgtcaggtaa aacagccagg acggctgttg cccttcctgc acaatgtagt cacgcgctaa
    14341 aaaatgaaac agccgcgtat cggctgaaac atccgttaaa aagcaatcct taaacgcgga
    14401 ttcactcatc aattgctgct taagatcatt attgtggatt tctttcacga tgtttatccg
    14461 ctatgagatt tctcatattt agcatacgta cgcggttggt aagattcaat catcggcccg
    14521 gtcgaatttt ctttgctgta gaaaaaataa cgcaactgga aacagaggaa ataaacaatg
    14581 gaaaagagaa aaattattct ggattgtgat ccgggtcatg atgatgctat tgcaatgatg
    14641 atggcagcga aacatccggc aatagattta ttaggcatca ctattgtagc gggtaatcag
    14701 acgcttgata aaacattaat taatggcctg aatgtttgcc agaaactgga gattaatgtt
    14761 ccggtttatg cggggatgcc gcaacccatt atgcgtaaac aaatcgttgc cgataatatt
    14821 cacggtgaaa ccggactgga tggcccggta ttcgagccgc tgacccgcca ggcagaaagt
    14881 acccacgcgg tgaaatacat cattgatacc ctgatggcaa gcgatggcga tatcactctg
    14941 gtgccggttg gtccgctttc aaatatcgcg gtggcaatgc gtatgcaacc cgcgatcctg
    15001 cccaaaatcc gtgaaattgt gctaatgggc ggcgcttacg gtacaggcaa cttcacgcca
    15061 tctgccgagt tcaacatctt tgctgatccg gaagcagccc gcgtggtgtt cacctccggc
    15121 gttccattag tgatgatggg cctcgatctc accaaccaga ccgtttgcac cccggacgtg
    15181 attgcacgga tggaaagggc aggcggccct gccggagagc tgttcagcga catcatgaac
    15241 ttcactctca aaacgcagtt cgaaaactac ggcctggctg gcggcccggt gcacgacgcc
    15301 acctgcatcg gttatctgat taaccctgat ggcattaaaa cacaggagat gtacgtcgaa
    15361 gtggacgtca acagtggccc ttgctatggg cgtaccgtct gcgacgagct gggcgttctt
    15421 ggcaagcccg ctaataccaa agtcggcatc actattgata ccgactggtt ctggggctta
    15481 gtcgaagagt gcgtgcgcgg ctacatcaaa acccattaag tgttgataac aagccgggtc
    15541 agtatcgcaa gatccaagcc cggctatttc tgatgggcag aaagccgtcg cccaatatga
    15601 catgaagaga taataactat ggatgtcatg agaagtgttc tgggaatggt ggtattgctg
    15661 acgattgcgt ttttgctgtc agtaaacaag aagaagatca gcctgcgtac cgttggcgcg
    15721 gcgttagtgt tacaggtcgt gattggcggg attatgcttt ggttaccgcc agggcgttgg
    15781 gtcgctgaaa aagtcgcttt tggcgtgcat aaagtgatgg cgtacagcga cgcgggtagc
    15841 gcatttatct tcggttcgct ggtcgggccg aaaatggaca cgctgtttga tggcgcagga
    15901 tttatctttg gtttcagggt attaccggca attatcttcg tcactgcact ggtgagtatt
    15961 ctctactaca tcggtgtgat ggggatttta attcgcattc tcggcggtat attccagaaa
    16021 gcattaaata tcagcaagat tgagtcattc gtcgcggtca ccaccatttt cctcgggcaa
    16081 aacgaaattc cggcgatcgt caaacccttt atcgatcgtc tgaatcgcaa tgaattattt
    16141 acagcgattt gtagtggcat ggcctcgatt gctggttcga caatgattgg ttacgccgcc
    16201 ctgggcgtac ctgtggaata tttgctggcg gcatcgttaa tggcgatccc aggcgggatc
    16261 ttgtttgccc gcctgttaag cccggctacg gaatcttcgc aggtttcctt taataacctc
    16321 tctttcaccg aaacaccgcc aaaaagcatt attgaagccg ctgcgacagg ggcaatgacc
    16381 gggctgaaaa tcgccgccgg tgtagcgaca gttgttatgg catttgtcgc catcattgcg
    16441 ttaattaatg gtattatcgg cggcgttggc ggctggtttg gttttgcaca tgcctcgctg
    16501 gagtccattt taggttacct gttggcccca ttggcgtggg tgatgggggt tgactggagt
    16561 gatgcaaatc ttgccgggag tttgattggg cagaagctgg cgatcaatga atttgtcgct
    16621 tatctcaatt tctcgccata tctgcaaacg ggtggcactc tggatgctaa aaccgtggcg
    16681 attatttctt tcgcgttgtg cggtttcgct aactttggtt ctatcggggt ggtggtgggg
    16741 gcgttttctg cggttgcgcc acaccgtgcg ccggaaatcg cccaacttgg tttacgcgcg
    16801 ctggcggcgg cgacactttc taacctgatg agtgctacta ttgcaggatt ctttattggt
    16861 ttagcgtagt gcgcaattta tgagccggat aagatgcggt caagcgtcga atccggcaac
    16921 attgttacac cattggcact aatgaaagcg cgttatcggc agacagggta gggttgttta
    16981 agctgccgct ggcgcgcgaa attgccgcgc aggctaccgc aaaacgggcg ctgtcgcgga
    17041 aactgtatcc ttcaagaaag ctatatacca ggcccgccat aaaaccatcg tcagcaccaa
    17101 aactgtctac tgtcgtatgc gctggcgcgg tcagcaaaaa ttgttctcca tccttatcgc
    17161 tgcaaaatac agattcatct ttcagataaa caaaaatgcg tttaacacct tgttgatgca
    17221 gcgaatttac agctctgatg cgatcgttgt cgtctttaat cggttgcccc cacaaaattt
    17281 ccagttcatt ttgtgttggt ttcagagtgt ggatccggct gtaccagttt tttactttgt
    17341 ttgctttaaa ttcggaaacg gtatcgacaa acatcggaat ttcatcagca atggtaaaga
    17401 cccactccag ggcttcaggt gtgagattac aatcagccag caccacacca gcatggcgaa
    17461 tcaagtcccg tgaagtattg agcagttgag gcgttagctg ttgcagaata tgtgtgtcat
    17521 tgattgccag gatagtttct tcctgcttat tagcgatagc gagataagtt gcagtgctgt
    17581 gtccatgcag acgaatgcag tttgaaacgt tcacgcccgc gcggcgcgtt tcttccagta
    17641 atgtctcgcc gtaaaaatcg ttgccaatag ctgatattaa atgcacatcc cgaccaagca
    17701 gagccaggtt atgcgcgata ttacgtccga cgccgcctgc ggaacagtga acagaacctg
    17761 gatgagaggc tgcttgcggg taacggatat ccgccattcc gcgaatatcc atattgattg
    17821 cccccacaac cacacagtaa tcctgctcgg taaggatata tcctttgcct ttaatcaaac
    17881 ccttgcgcat caaatccata atatgagcgg caacacgtga gcggctgatt tgcagaatat
    17941 cggcgatttc attctgttga atcagcgggt tacgccgtaa aattttaaga atctgctttt
    18001 ccctgtcgtt catcgatcag gccaccgctt tttcagtttg ttgcgctttc agccaggcga
    18061 tctcttctgc ccagatatcc gggttgatgg tttcaaggat cagcggaatg ccgtcgaaac
    18121 ggtcgtcttg catgatccag cggaacgcat catgaccgat attgccttca ccgaggctat
    18181 gatggcggtc aacgcggctg ccaaaggtac ttttcgcatc gttaaggtgc atcccgcgca
    18241 gatacttaaa gccgacaata cgggcaaaat cagcgaacgt tttttcgcat tcggctggag
    18301 tacgcaaatc atacccggca gcgaaagcat ggcaggtatc aatgcagacg ccgacacggg
    18361 atttatcttc cacgccgtcg ataatcgccg cgagatgttc gaatttaaac cctaagttac
    18421 tgccctgacc ggcggtgttt tcaatcaccg ctgttacacc ttgagtttta tccagcgcaa
    18481 tgttgatgga ttcggcaata cgcgcaaggc aatcctcttc tgaaatctgc atcagatggc
    18541 tgccagggtg gaagttgagc aaagaaagcc ccagttgttc gcaacgctgc atttcatcta
    18601 taaaggcatc gcgcgatttt tccagagctt cagtgaccgg atgtccgagg ttaatcagat
    18661 agctgtcgtg gggaagaatt tgcgccgatg tgtagtgata tttttcacag gccgctttga
    18721 attcatcgat ggtttgcgtc gtgagcggtg cggcacgcca ctgacgttgg tttttggtga
    18781 acaaggcaaa cgcggttgcg tcgatttcgg cggcgcgaat tgcggcattt gccagaccgc
    18841 cagcagcact aacgtgcgct ccaatgtatt tcatgcgagg actcctgtta aacccgctgg
    18901 aggaaaacgg taatgatagc gggttaacag gagcaagatg tagtggttta tgcgatgacg
    18961 ctttgaatca catagttaat cgcaccacca ccaacaatca gccaggcaaa cagtaccagt
    19021 gccatcagca gaggtttcgc cccggctttt ttcagcgcgc tgacgtgagt ggtcagaccc
    19081 agcgccgcca tcgccattgc cagcaggaag gtatccagcg ttaccagcat gttcaccacg
    19141 ctctgcggta acaggtggaa cgagttaaag atggcaacta cgatgaacaa gatggcaaac
    19201 cacggaatag tgattttgct tttctcgccg ctgttcgccc cagacagctg tttaacacgc
    19261 gccgccagca ggatgaggaa cggagccagc atcatcacgc gcagcatttt ggaaataact
    19321 gctgcgtttt ccgcatccgg gctgatggca tgacctaccg ccaccacctg cgccacttcg
    19381 tgcacagtag aaccaatgta gataccgaaa gtttccggac taaaccattg agacatcagc
    19441 ggatatatcg ccgggtagag gaaaatcgcg acggtcccga agataacaac ggttgcaaca
    19501 gccacggtta ctttactggc ttccgctttc actaccggct cagtcgccag taccgcggca
    19561 gcaccacaga tactgctacc ggcaccgatc aaccagctgg tgtgcttatc cagaccaaac
    19621 actttctgcc ccaggaagca agccagcagg aaggtactgg acagcgtcaa cacgtcaatg
    19681 atgatcccac tgataccgac atcggcaatt tgcgagaacg tcagacggaa gccataaaga
    19741 atgataccca gacgtaataa atattgcttg gcaaacagca caccaccgtc acagcttttc
    19801 cagatgtgcg gatagatggt gttgcctaaa accatcccca acaagattgc gagggtgagg
    19861 gcactaaacc cggcacccgc aaccgcggga atggaaccac cccacagggc gaccccggtg
    19921 ataactgcac tcagggctaa ccccggaata aaatgccaca gtgtacgatg ttgtttctgt
    19981 aaggtgatat tcgtcataac cctctccttt aaccgggcat aaggttacga ctaactggtt
    20041 taaaaataaa attgattata tatttataat taatctttat aagtggtaag cgactatgca
    20101 catcaccctc cggcagttgg aagtttttgc agaagtattg aaaagtggat caaccaccca
    20161 ggcgtcggtg atgctggcgt tgtcgcaatc agcagtgagc gcagccttga ccgacctgga
    20221 agggcagctt ggcgtgcaac tgtttgatcg cgtggggaaa agactggttg ttaatgaaca
    20281 cgggcggctg ctctatccgc gtgcgttggc attgcttgaa caggcggttg aaatcgaaca
    20341 actgtttcgc gaagacaacg gcgcgattcg tatctatgcc agtagtacca tcggtaacta
    20401 cattctgcct gcagttatcg cccgttatcg ccatgattat ccgcagttgc cgattgaact
    20461 tagcgttggg aacagccagg acgtgatgca agcggtgctg gatttccgcg ttgatattgg
    20521 ttttattgaa ggaccgtgcc acagcactga aattatttct gaaccgtggc tggaagacga
    20581 gctggtggtt ttcgccgcgc cgacttcgcc gttggcccgt ggtccggtca ccttagagca
    20641 gctggccgct gcgccgtgga tcctgcgtga acgcggttcc ggcacgcggg agattgtcga
    20701 ttatctattg ctgtcacatt taccgaagtt tgagatggcg atggaattag gtaactccga
    20761 ggcaatcaaa catgcggtgc gtcatgggtt gggaattagt tgcctgtcgc gacgggtgat
    20821 tgaagatcaa ttgcaggcag gcacattaag tgaagttgcg gtccctctgc cgcgcctgat
    20881 gcgtacgttg tggcgtatac atcatcggca aaaacacctt tccaacgcgc tacggcgctt
    20941 tctggactat tgcgatcccg cgaatgtacc gcgttaagtt gctgtacaag aacatgctgg
    21001 tgctgtgtcg atttcgtgac gcagcgccct cagcatgcat tcgccagaaa agagattggc
    21061 tgctttactt ataatccctg ggcgatcatg aaggtgtctt ataaccgtgt atttctgccg
    21121 gaaggattgc caatcgtctg ctacaatcgc gcctcatttt taagatggat agcatttttg
    21181 tatggtttcc gaaactaaaa ccacagaagc gccgggctta cgccgtgaat taaaggcgcg
    21241 tcacctgacg atgattgcca ttggcggttc catcggtaca ggtctttttg ttgcctctgg
    21301 cgcaacgatt tctcaggcag gtccgggcgg ggcattgctc tcgtatatgc tgattggcct
    21361 gatggtttac ttcctgatga ccagtctcgg tgaactggct gcatatatgc cggtttccgg
    21421 ttcgtttgcc acttacggtc agaactatgt tgaagaaggc tttggcttcg cgctgggctg
    21481 gaactactgg tacaactggg cggtgactat cgccgttgac ctggttgcag ctcagctggt
    21541 catgagctgg tggttcccgg atacaccggg ctggatctgg agtgcgttgt tcctcggcgt
    21601 tatcttcctg ctgaactaca tctcagttcg tggctttggt gaagcggaat actggttctc
    21661 actgatcaaa gtcacgacag ttattgtctt tatcatcgtt ggcgtgctga tgattatcgg
    21721 tatcttcaaa ggcgcgcagc ctgcgggctg gagcaactgg acaatcggcg aagcgccgtt
    21781 tgctggtggt tttgcggcga tgatcggcgt agctatgatt gtcggcttct ctttccaggg
    21841 aaccgagctg atcggtattg ctgcaggcga gtccgaagat ccggcgaaaa acattccacg
    21901 cgcggtacgt caggtgttct ggcgaatcct gttgttctat gtgttcgcga tcctgattat
    21961 cagcctgatt attccgtaca ccgatccgag cctgctgcgt aacgatgtta aagacatcag
    22021 cgttagtccg ttcaccctgg tgttccagca cgcgggtctg ctctctgcgg cggcggtgat
    22081 gaacgcagtt attctgacgg cggtgctgtc agcgggtaac tccggtatgt atgcgtctac
    22141 tcgtatgctg tacaccctgg cgtgtgacgg taaagcgccg cgcattttcg ctaaactgtc
    22201 gcgtggtggc gtgccgcgta atgcgctgta tgcgacgacg gtgattgccg gtctgtgctt
    22261 cctgacctcc atgtttggca accagacggt atacctgtgg ctgctgaaca cctccgggat
    22321 gacgggtttt atcgcctggc tggggattgc cattagccac tatcgcttcc gtcgcggtta
    22381 cgtattgcag ggacacgaca ttaacgatct gccgtaccgt tcaggtttct tcccactggg
    22441 gccgatcttc gcattcattc tgtgtctgat tatcactttg ggccagaact acgaagcgtt
    22501 cctgaaagat accattgact ggggcggcgt agcggcaacg tatattggta tcccgctgtt
    22561 cctgattatt tggttcggct acaagctgat taaaggaact cacttcgtac gttacagcga
    22621 aatgaagttc ccgcagaacg ataagaaata agtttcctcc cttccttgct aagccctctc
    22681 aaccgagagg gctttttcaa ttccatttcc ctgacaaatc atgcggatat aaaatttaac
    22741 atttggattg ataattgtta tcgtttgcat tatcgttacg ccgcaatcaa aaaaggctga
    22801 caaatcagag gctgttccgg ctttctggga tgatcacctg catacaaata agtccaccgc
    22861 gatgctgccg tacgcaaggg gacgtgaaga agatgtgagc gataacccat tttattttcg
    22921 tagttacctc atggagatat ggaatgttta ggttgaaccc tttcgtacgg gtcgggctgt
    22981 gtttgtccgc tatttcttgt gcatggcctg tgttagcggt cgatgatgat ggcgaaacga
    23041 tggttgtcac tgcatcttcc gttgaacaaa acctcaaaga tgcacccgcc agtatcagcg
    23101 tcattaccca ggaagacctg cagcgaaaac cggtacagaa tctgaaggat gtcctcaaag
    23161 aagtgcctgg cgtacaactg acgaacgaag gggataaccg taagggcgtt agtattcgtg
    23221 gtctggacag cagctacacc ctgattctcg tcgacggtaa acgcgttaac tcccgcaatg
    23281 ccgtcttccg ccacaatgat ttcgatctga actggatccc ggtcgattcc atcgaacgta
    23341 ttgaagtggt tcgtggcccg atgtcgtcgc tatacggctc cgatgcgctc ggcggtgtag
    23401 tgaatatcat caccaaaaaa atcggtcaga aatggtcggg taccgttacc gtcgatacca
    23461 ccattcagga acatcgcgat cgcggtgata cctataacgg tcagttcttt accagtggac
    23521 cattaattga tggtgtgctg ggaatgaaag cttacggcag cctggcaaaa cgtgaaaagg
    23581 atgacccgca aaactcaacg accaccgata ccggagaaac gccgcgtatt gaaggattct
    23641 ccagccgcga cggcaatgtc gaatttgcct ggacaccgaa tcaaaatcac gattttactg
    23701 ccggatacgg tttcgaccgt caggatcgtg attccgactc gctggacaaa aaccgcctgg
    23761 aacgccagaa ctactccgtc agccataatg ggcgttggga ttacggcacc agcgaactga
    23821 aatactacgg tgagaaagtc gagaacaaaa accctggcaa cagcagcccg ataacttccg
    23881 aaagcaatac ggtcgacggc aaatacacgt tgccgctgac ggcgattaat cagtttctca
    23941 cggttggcgg tgaatggcgt cacgacaaac ttagcgatgc ggtgaacctg accgggggaa
    24001 ccagctccaa aacgtctgcc agccagtacg cgctgtttgt ggaagatgaa tggcggatct
    24061 tcgagccgct ggcgctgacg accggcgtgc gtatggacga tcacgaaacc tacggtgaac
    24121 actggagtcc gcgtgcctac ctggtttata acgccaccga caccgtaacg gtgaaagggg
    24181 gctgggcgac ggcatttaaa gcaccttctc tgttgcaact tagccctgac tggacgagca
    24241 attcctgccg tggcgcatgt aagattgtgg gtagcccgga tctgaaacca gaaaccagcg
    24301 aaagttggga gctggggctt tactacatgg gtgaagaagg ctggctggaa ggggttgaat
    24361 ccagcgttac cgttttccgt aacgatgtga aagatcgtat cagcatcagc cgtacgtctg
    24421 acgtcaacgc tgcaccgggc taccaaaact ttgttggttt tgagacgggc gctaacggac
    24481 ggcgcatacc ggtatttagc tactacaacg ttaacaaagc tcgtattcag ggcgtggaaa
    24541 ccgaactgaa aattccgttc aacgatgaat ggaaactgtc gatcaactac acctacaacg
    24601 atggtcgtga tgtcagcaac ggcgaaaaca aaccgctatc tgccgttcca tactgctaac
    24661 ggtacgctgg actggaaacc gctggcgctg gaagactggt cattctatgt ttctggtcac
    24721 tataccgggc agaaacgcgc cgacagcgcg acggctaaaa caccgggcgg ttataccatc
    24781 tggaataccg gcgcggcctg gcaggtgact aaagacgtca aactgcgcgc aggcgtgctg
    24841 aaccttggcg acaaggatct cagtcgtgac gactacagct ataacgaaga cggacgtcgt
    24901 tactttatgg cagtggatta tcgcttctga tgagaagatg cccggcgaac cgggcggact
    24961 ttcacttcag taaatactgc gcatggaagc gcaggtgatc ctctataaaa gaggcgatga
    25021 agtagtaact gtgatcatat cccggctgaa tacgcagcgt catcggccac gctttctggc
    25081 gcgcggcttc tgccagtacc gcaggttgca actggtcggc aagaaactga tcattatcgc
    25141 cctgatcgat aagcgtcggg atcgcatcct gcgcgttact ggcatacatc agcgcgcaac
    25201 tgtcccattc cagccatgca tttttgtcct cacctaaata gctgctaaac gctttgattc
    25261 cccacgggac gctgcacgga ttcacaattg gcgcaaaggc cgaaacgctg gtgtatttac
    25321 ccggattttt cagcgccata atcagcgcac cgtgaccacc cattgagtga ccgctaatgg
    25381 cgcagcggtc gctgacatta aattgcgact gaaccagcgc cggtaattca tcgcgcagat
    25441 aatcatacat ccggtaatgc gtcgcccacg gcggttgcgt ggcattaaga taaaagcctg
    25501 cgccctggcc taaatcgtag ccatcgtcgt tggcaacctt ttcgccgcgc gggctggtgt
    25561 ctggcatcac cagtacaatc cccagttctg ccgccacgcg ctgggcaccc gccttggtgg
    25621 tgaagttctc gtcattgcag gttaatccgg aaagccagta cagcactggt ggcggggtgt
    25681 gatcacgagg gggtgggaga aagatactga acgtcatcgg gcagtttaag gtactggagt
    25741 cgtgtcgcca gcgttgctgc cagccttcaa aacagcggtg ctcttcgagc atttccatgc
    25801 gtggctcctt gttgtgttgt ttgcaatttc ttcatcatac cgtttattca atgtgctgtg
    25861 agtaactttc acttccgtat ttgcataacg atgttttaac atctgctgat gaaaggcagc
    25921 ggcaattaca ataattatcg ctgtgaatac tggattatgt gcgccgcctc acgcacaata
    25981 atcaggctgt aaatcagctt aataactttg cccccacgca gggcggaggc gtcacacctg
    26041 caggagaaat cataaatgcc atcactcagt aaagaagcgg ccctggttca tgaagcgtta
    26101 gttgcgcgag gactggaaac accgctgcgc ccgcccgtgc atgaaatgga taacgaaacg
    26161 cgcaaaagcc ttattgctgg tcatatgacc gaaatcatgc agctgctgaa tctcgacctg
    26221 gctgatgaca gtttgatgga aacgccgcat cgcatcgcta aaatgtatgt cgatgaaatt
    26281 ttctccggtc tggattacgc caacttcccg aaaatcaccc tcattgaaaa caaaatgaag
    26341 gtcgatgaaa tggtcaccgt gcgcgatatc actctgacca gtacctgtga acaccatttt
    26401 gttaccatcg atggcaaagc gacggtggct tatatcccga aagattcggt gatcggtctg
    26461 tcaaaaatta accgtatcgt gcagttcttt gcccagcgtc cgcaggtgca ggaacgtctg
    26521 acgcagcaaa ttcttattgc gctacaaacg ctgctgggca ccaataacgt ggctgtctcg
    26581 atcgacgcgg tgcattactg cgtgaaggcg cgtggcatcc gcgatgcaac cagtgccaca
    26641 acaacgacct ctcttggtgg attgttcaaa tccagtcaga atacgcgcca cgagtttctg
    26701 cgcgctgtgc gtcatcacaa ctgattaaaa ggcaggaacc atggagcgca acgtcacgct
    26761 cgattttgtt cgcggcgtcg ccattctggg gatcctgcta ttaaacatca gcgcctttgg
    26821 gctaccaaag gcggcttatc tcaatcccgc ctggtacggt gctattacgc cgcaggatgc
    26881 atggacctgg gcatttcttg atctcatcgg ccaggtgaaa ttcctcacgc tttttgcgct
    26941 gctgtttggt gctggactgc aaatgttgct gccccgtggc agacgctgga tccagtcgcg
    27001 gttaacgctg ttagtcttat tgggctttat tcacggttta ttgttctggg acggcgatat
    27061 tctgctggct tacgggctgg tgggcttaat ctgctggcgg ctggtgcgcg atgcgccatc
    27121 ggtaaaaagc ctgtttaata ccggcgtcat gctttatctg gtggggcttg gcgttttgct
    27181 gttattgggg ctgatttccg acagccagac cagccgcgcc tggacgccgg atgcatcggc
    27241 tattttgtat gaaaaatact ggaagcttca cggcggcgtt gatgcgatca gtaatcgtgc
    27301 cgatggtgtt ggcaacagtt tactggcact gggcgcacag tatggctggc aactggctgg
    27361 gatgatgctc attggtgcgg cgctgatgcg cagcggctgg ctgaaagggc agttcagctt
    27421 acgtcactat cgtcgtactg gttttgtgct ggtggcgatt ggggtgacca ttaaccttcc
    27481 tgccatcgcc ctgcaatggc agctggactg ggcgtatcgc tggtgtgcct tcttacttca
    27541 aatgccgcgg gaactgagtg cgccgtttca ggcgattggc tatgcgtcgc tgttttatgg
    27601 cttctggccg caattaagcc gctttaagct ggtgctggca atcgcctgcg tcggacggat
    27661 ggcgctgacc aactatctat tgcaaacgct gatttgtacc acgctttttt accacctcgg
    27721 tttgtttatg cattttgatc gcctggagct gctggcgttt gttattccgg tatggctggc
    27781 gaatatcctc ttctctgtta tctggctgcg ttacttccgc caggggccgg tggaatggct
    27841 ctggcgtcag ttaactttgc gtgctgccgg aacggcaata tctaaaacat caagataacg
    27901 atctggatca cattcgttaa caaaacggct gtaaccgttt ccattgctgt gactcgattc
    27961 acgaagtcct gtattcagtg ctgacaaaat agccgccagc aagcagtcat ttactgcaat
    28021 ctcataacag gtagtgaata tgatcaccat tcgtgatgta gcgcgtcagg ctggcgtctc
    28081 tgtggcaacg gtttcccggg tgctcaataa cagcacgctg gtcagtgccg acacgcgtga
    28141 agcagtaatg aaagccgtga gtgagctgga ttatcggcca aatgccaatg cccaggcgct
    28201 ggcaactcag gttagcgata ccattggcgt ggtggtgatg gacgtttctg atgctttttt
    28261 cggcgcgctg gtaaaagcgg tggatctggt cgcccagcag catcagaaat acgtgctaat
    28321 cggcaatagc tatcatgaag cggaaaaaga gcgtcacgcc attgaggtgt taattcgcca
    28381 gcgttgtaat gcgttgattg ttcactcaaa agcattgagt gacgatgaac tggcgcaatt
    28441 tatggataac attcccggta tggtgttaat caaccgcgtt gtgccggggt acgcccatcg
    28501 ttgcgtttgc ctggataatc tcagcggtgc ccgaatggcg acgcgcatgt tgctgaataa
    28561 cggtcatcaa cgtattggtt atctttcttc cagccacggc attgaagatg acgccatgcg
    28621 taaagcaggc tggatgagtg cgttgaaaga gcaggatatt attccgccgg aaagctggat
    28681 tggcactggt acgccggaca tgccgggcgg tgaggcggcg atggttgaac tgctggggcg
    28741 caatctacaa cttaccgctg tatttgctta taacgacaat atggccgctg gcgcactgac
    28801 agcattaaaa gataatggca ttgcgattcc gttacatctc tcaatcatcg gtttcgatga
    28861 tattcccatc gcccgttaca ccgacccgca attaacgacc gtgcgttatc ccattgcttc
    28921 aatgactaaa ttagccaccg aactggcctt gcagggggca gcaggcaata ttgatcctcg
    28981 tgccagccac tgttttatgc cgacgttagt gcgtcgtcat tctgtcgcaa cgcgccagaa
    29041 tgcggcggcg atcactaact caacaaatca ggcgatgtaa ccgctttcaa tctgtgagtg
    29101 atttcacagt atcttaacaa tgtgatagct atgattgcac cgttttaacg ttgtaacccg
    29161 tatgtaacag tgaataatca ctttcaccga ggtaatagcg tcataacaac aattaaagcc
    29221 gttttctgga gcgttaccgg gcatggaaga acgaatttta aaaagtgagc ttcggcgttc
    29281 agtaacactt cattaactct actgccccgc cgagcattta tctcaagcac taccctgcat
    29341 aagaaaaacc ggagatacca tgaataagaa ggtgttaacc ctgtctgctg tgatggccag
    29401 catgttattc ggtgccgctg cacacgctgc tgatactcgc attggtgtaa caatctataa
    29461 gtacgacgat aactttatgt ctgtagtgcg caaagctatt gagcaagatg cgaaagccgc
    29521 gccagatgtt cagctgctga tgaatgattc tcagaatgac cagtccaagc agaacgatca
    29581 gatcgacgta ttgctggcga aaggggtgaa ggcactggca atcaacctgg ttgacccggc
    29641 agctgcgggt acggtgattg agaaagcgcg tgggcaaaac gtgccggtgg ttttcttcaa
    29701 caaagaaccg tctcgtaagg cgctggatag ctacgacaaa gcctactacg ttggcactga
    29761 ttccaaagag tccggcatta ttcagggcga tttgattgct aaacactggg cggcgaatca
    29821 gggttgggat ctgaacaaag acggtcagat tcagttcgta ctactgaaag gtgagccggg
    29881 ccatccggat gcagaagcac gtaccactta cgtgattaaa gagctgaacg ataaaggcat
    29941 caaaactgaa cagttacagt tagataccgc aatgtgggac accgctcagg cgaaagataa
    30001 gatggacgcc tggctgtctg gcccgaacgc caacaaaatc gaagtggtta ttgctaacaa
    30061 cgatgcgatg gcaatgggtg cggttgaagc gctgaaagca cacaacaagt ccagcattcc
    30121 ggtgtttggc gtcgatgcgc tgccagaagc tctggcgctg gtgaaatccg gtgcactggc
    30181 gggcaccgta ctgaacgatg ctaacaacca ggcgaaagcg acctttgatc tggcgaaaaa
    30241 cctggccgat ggcaaaggtg cggctgatgg caccaactgg aaaatcgaca acaaagtggt
    30301 ccgcgtacct tatgttggcg tagataaaga caacctggct gaattcagca agaaataaga
    30361 ctgattgggc gcagtcgttt actgcgccct tattgtatgg ccaacaaggt aaaattatat
    30421 ggtcagctca acgactccgt cttccgggga atacttgttg gaaatgagcg gtatcaacaa
    30481 gtcctttcct ggtgttaagg cacttgataa cgttaattta aaagtccggc cacattctat
    30541 ccatgcatta atgggggaaa acggtgcagg aaaatcgaca ttattaaaat gcctgtttgg
    30601 tatttatcaa aaagactccg gcaccatttt attccagggt aaagagatcg atttccattc
    30661 cgccaaagag gcactggaaa atggtatttc gatggtacac caggagttaa acctggtatt
    30721 acaacgttcg gtgatggata acatgtggct ggggcgatat cccaccaaag gcatgtttgt
    30781 cgatcaggac aaaatgtacc gcgaaaccaa agcgattttt gatgaactgg atattgatat
    30841 cgatccgcgt gcgcgcgtcg gcacattatc cgtttcgcaa atgcagatga tcgaaatcgc
    30901 caaagcgttt tcctataacg cgaaaattgt gattatggat gaaccgactt cttcgttaac
    30961 cgaaaaagag gtcaatcatc tgttcactat tattcgtaaa ttaaaagagc gcggctgcgg
    31021 tattgtttat atctcgcata aaatggaaga aatcttccag ttatgtgatg aagttaccgt
    31081 attgcgcgac ggtcagtgga tcgccaccga accgctggca ggactgacga tggacaagat
    31141 catcgccatg atggttgggc gttctcttaa tcagcgtttt cctgacaaag aaaacaagcc
    31201 gggcgaagtc atcctcgagg tgcgtaacct aacgtcactg cgccagccgt cgattcgcga
    31261 tgtctcgttt gatctgcata aaggggagat cctcggtatt gccgggctgg tgggggcgaa
    31321 acgtaccgat attgttgaga cgttatttgg tattcgcgag aaatcggctg gcaccattac
    31381 gttgcacggc aaacagatca ataaccataa tgccaacgaa gccataaacc acggatttgc
    31441 actggtaact gaggagcgcc gctcaacggg aatttatgcc tatctggata ttggttttaa
    31501 ctcgttaatt tccaatattc gcaactacaa aaataaagtt ggtttactgg ataactcgcg
    31561 gatgaaaagc gatacccagt gggtgattga ttcgatgcgg gtaaaaacgc cgggtcatcg
    31621 gacgcaaatt ggttcgctct ccggtggtaa tcagcaaaag gtgattattg gtcgctggct
    31681 actaacgcaa ccagaaatat taatgctcga tgaaccgacg cgcggtattg atgtcggggc
    31741 gaagtttgaa atttatcagt taattgccga actggcgaag aaaggcaagg ggattattat
    31801 tatctcctct gaaatgcctg agttgttagg gataacagac cgtattctgg tcatgagcaa
    31861 tggtctcgtt tccggaattg tcgatacaaa aacaacaacg caaaacgaaa ttctgcgtct
    31921 tgcgtctttg cacctttaag attaggggct tcccatgagt gcgttaaata agaaaagttt
    31981 tcttacttac ctgaaagagg gcggtattta cgtcgttctt ttagttttgc tggcgattat
    32041 tattttccag gacccaacat ttttaagtct gttgaactta agtaatattc tcacccagtc
    32101 atcggtgcgt attattatcg cgctcggtgt ggcagggtta attgtcaccc aggggaccga
    32161 tctttctgct ggtcgtcagg tagggctggc ggcagtggtg gctgcgacat tattgcagtc
    32221 catggataac gccaacaaag tgttcccgga aatggcgacg atgccgattg cgctggttat
    32281 tctgattgtc tgtgccattg gtgcggtgat cggtttgatc aacggtctga ttatcgctta
    32341 tctcaacgtg acgccgttca ttaccacgct cggcacgatg atcatcgtct atggaatcaa
    32401 ctcgctctat tacgactttg tcggggcgtc gccaatttct ggttttgaca gtggcttctc
    32461 tacctttgct cagggctttg tcgcgctggg gagtttccgt ctctcttaca tcaccttcta
    32521 cgcgttgatt gcggtggcgt tcgtctgggt gttgtggaac aaaacccgct tcggtaagaa
    32581 catttttgcc attggcggta acccggaagc ggcaaaagta tctggtgtca acgtcggcct
    32641 gaacctgctg atgatctacg cgttgtctgg cgtgttctat gcctttggcg ggatgttaga
    32701 agccggacgt atcggctctg ccaccaacaa cctcggcttt atgtatgagc tggatgctat
    32761 cgcggcgtgc gtggtaggcg gtgtatcgtt cagcggcggt gtggggacgg tgattggcgt
    32821 ggtgaccggg gtaattattt ttaccgtcat caactatggc ctgacgtata tcggcgtaaa
    32881 cccatactgg cagtacatca tcaaaggggc gattattatc ttcgccgtag cgctggattc
    32941 actgaaatac gcgcgtaaga agtgatgtga ttgtggatat tgccggatgc gctgcatccg
    33001 gcaatcggtg ccaaatgccg gatgcgtctc tggttttaca acgttaccgg gtgttctttc
    33061 tcgcctttct taaacttcac ttccccgagc tcaatacaac ccaccgggca gacgtgacca
    33121 caaagcagac aacccacaca tttctcggta ttacaatgcg gtgtgcgggt tttctcgctc
    33181 cattccatcg cctggtgacc gccgtcgtaa caggaaatat aacagcgtcc acagccaaca
    33241 catttatcaa gattgatacg gggatagaca atataactgc ggtctaaatc ttccgccggg
    33301 acaatattgt tattcgccag acctaccatc tcctgcagcg aatcaaatcc ctgatcggcg
    33361 agataatgcg acaacccgct tgccatatct tccactatcc gatacccgta ctgcatgatg
    33421 ccggtggtca cctgtaacgt tgctgcgccg agcaataaaa actcagccgc atcctcccag
    33481 gtttcaatgc cgccgatacc gctgattggg aaatcgcgca gttctggatg ggtgcgcatc
    33541 tgctgaatga agcgcagggc gatcggtttt accgctttac cggaatatcc ggaaatactc
    33601 gattttccgt taacgatcgg cataccgatt ttctgattaa gatcgatatt ggtgatggat
    33661 ttaacggtgt taatcgccgc aatgccatct gcgccgccgc gcttcgccgc cagcgccact
    33721 tcgcacatat caccgatatt cggcgtcatc ttcgccagca ttggcagcgt ggaaccccgt
    33781 ttcactgccc gacaatattt ttctaccagc tccgggcttt gcccgacatc gctacccatc
    33841 gcatgagaag tcatttgcgg acaggagaag ttacactcga tcatatccgc gccagcttct
    33901 tgcaccaggc gcgccagctc ctcccattgc tgctcatttt cccccatgat cgaagcgatc
    33961 aatactttgt ccgggtaatc ttccttcagc tgacgcaggg cggccagatt ctcttccaac
    34021 ggatgttcag caatctgctc catatttttg aagccgataa aaccggtatc ttctttcacc
    34081 agatgatcaa aacgcggcga gacttcgttg gcgataaaaa agccgatcgt tttaaacacc
    34141 acgccgcccc aacctgtgtc gtaggctttg gcacacatct catagcagtt gcctacaggc
    34201 gaagaagaga ggcagaacgg gttgggaaac ttcacgccgc aaaaagtaat cgaaagatct
    34261 ttcgttaaca tgagcaagct ccctctaaat agtgatgaat cgcctcggcg gcttctttcc
    34321 cggttttcac ggcatagacc acggttttgt caccctcaac aatatcgcca gcagcaaaga
    34381 cttgcggatc gcgggtctga taattttgtg ttttgatggt gttacgctgc ggctctaact
    34441 ccgcaaaggc atccagtctg gcatgctgac cgacggcgag aataatttta tctgccgcca
    34501 tcgtcagttc gcccggtaac cgtacatgct taaacgtgac tttattgcct tccacggcta
    34561 ctggcgtgaa tccatcgatg atcgaaacgc ccagctcccg ggcgctggca aactcttttt
    34621 cgcttgccgg aaactcatct aactcttcac gcgctacgca ggttaccgcc tgacagccga
    34681 gaactttcag cgtgctggct acgtccatcg caacatcacc gccgccgata attaatgcgc
    34741 tttgtggaat gctgatatcg ccttgcgcct gtcgtgcaca ttgcaggaag tcgacggcaa
    34801 tctcaacgtc gctatgctca aacagcgata acccggaacc gctcgataac cccacggtga
    34861 ccagtaccgc gcggttttct gctttcagct gctcaagggt gagtgtgtta ccgacttcgt
    34921 tgttgcactt aatggtcacg cccatttttt caatacgggc gatctctgca tccagcactg
    34981 attgcggtaa ccggaattgc ggaataccgt tacgcaacca gccaccgggg tgcgcttctt
    35041 tctcataaat cgtgacgtca taaccctggt ttgtcagtgt tacactggcc tgcaatccgg
    35101 caggacctgc gccaataatc gcgactttgc cgagcgtttt agtaccgggc tgataaattt
    35161 ccatcccggt ttgttgttca aaatcagtaa caaaacgctg taagcgaccg atatcaatgg
    35221 gagtatcaac accggcacgg gtacaaccgc tttgacataa tttctccgtc gggcacactc
    35281 tggcacaaac ggcaccgagg gcattatttt cgcgaattgt ctcggcagcg cctttaaaat
    35341 tacgaaagta gattgagcga ataaatttcc ccggatcggt ctgtgccggg caagcctgac
    35401 tacagggagc gtcatgacat aataaacagc gagaggcttc tttaatcgcc agtaaaggcg
    35461 taaaagccgg agtgagttca tccagataat tttgttgcgg catgtgatct tccttaatgc
    35521 tatgagtaat taaggcgaac gctaaatata ctcgtcatac ttcaagttgc atgtgctgcg
    35581 tctgcgttcg ctcaccccag tcacttactt atgtaagctc ctggggactc actcacttgt
    35641 cgccttcctg caactcgaat tatttagagt ataaatattg cgtgctgata ttaaggctat
    35701 gcgcgtgggg aaatttatac tgtgaaagga ttcacaaata gctgcggttc agaatgttat
    35761 atccgcagct tattgagtga agtaataatt attctcaata attaacagaa aagagaaaat
    35821 attaacgata gaaaataaga taaacaatca tgccgataga taaaattccg aagccgatat
    35881 atgccaccat ataagcgaaa aagagtccgg gattttttgc tcgccagaca tacaccggtt
    35941 ccggtgcatt tttaacccgt ttatcaatcg cgcctgcgcg ccagcctttc catgcttcgc
    36001 ggaaacaaaa tatcggaata aggaaaaaaa cggcaacgac aaaaaactgc tgtacatcca
    36061 taactacttt ccttgtttct tttgtaattc aagtaactgt tcgggtgtga cggcgggata
    36121 cccctgcgca tcttccggca cctggtgcat agccggaata gggaccagcg gccctaaaaa
    36181 acgcggttca cgtttaaaaa tataaaggtc agccagcgca ccgaaacggg cggcaaattc
    36241 gcgaatacgt actgacagca tatctttcgg tgacggtacg gcataacact gagcctgaat
    36301 ccccatatgc agcgcaataa ataatgcccg ctcacagtgg aaacgttggg tgataataat
    36361 gaaatcatta gtatcgaaaa ctttgcgtgt acgcacgatg gaatccagcg tacgaaagcc
    36421 tgcgtaatcg agaacaatat ctgatgggtc gacaccagca gcgattaaat ctttgcgcat
    36481 ggtcatcggc tcattataac tttgcaatgc gttatcgccg ctcagtaata gataatttac
    36541 cttaccgctg ttataggcat taatcgctcc ttgaatgcgg tagcgataat actgattaat
    36601 tacgccagta cgataatatt ttgctgttcc gagcaccaca ccgacctggc ggtaggggag
    36661 atcctgcaat tcgtcgtaga tataaggcgc ggttttccag ctcatccagc gatcgaggcc
    36721 gagcacagtc aacagcagca agccgatcag gactaacagg ctgaggaaca cgcgctttaa
    36781 catgcaggag actcgatcta aggaaaattt ccatcaggct actttacccg ctggttttgc
    36841 gcaagaaacg gcaaactgtt tgccgggaat ttcgacacca ggagagggct ggcaagccgg
    36901 acaaggcgtt cacgccgcat ccggcaccag gcttaagcga gaagcactcg gtcgatactg
    36961 tgacagccga gagctttcag cgttgcggag gtggcatccc actgaatcaa cggcgcatcg
    37021 gctttttctg ccagaaccgc gcgctggata tccgggtaat cataaccctt gagattcaac
    37081 agaattaacg ctccctgcaa cggtggcaga gtcgggttga atgcggcgtt ttcagcgtag
    37141 ctgccactga aaatacgacc gtctttacat tccagcgcga caccgcttgg cgacttactg
    37201 taaggcatgt gcgaacggtt tgccgctgca atcgccgcct gagaaagcgc atcaccagtc
    37261 agcgcatagc cgtgatcctg ctcgtccatc agcagcgttt taatctccag atctttcggc
    37321 ccaaaggcat ccggcagata gtcacgcagc gcgtgtgcct cgcggcccgg cagatgaata
    37381 cgcagatcca gaccgctgtt cagttcattc ataaactgac ggcagtgacc acaaggcgtg
    37441 tagttaacgg tgatggctgc aagcgctttt tcaccactca accaggcgtg gctgatcgcg
    37501 ctttgttcgg catgaacggt ttgctgcatt gtcgcaccaa taaattccat attggcaccg
    37561 aaataccagg ttccgctcac accgcgcgca attgcgccaa cattaaaatt cgacaatggc
    37621 gtacgcgcac aggccgccgc cagcggaagt agtgcgaatg ccagcgcgtc ttcgtccagc
    37681 cccgttgcgc tcttcagcga tgagacttgc tccccggtca acaaagcggg gaagtacttg
    37741 tctgccagaa taggttccag tgcagattgc aagttatccg caagttgggc aaaagcggtt
    37801 tgaaaacgtg gatgcatggc gttgcctcat aatcatgtaa tgggataaca gtttacgaac
    37861 ccgttatggc tttatatgtg atctgaatct cattaattat gcaacgtgtg aaacttacat
    37921 caaaaatgcg cgacgcatcg caaattttag cccattactg ccagaataat cgggaaaagg
    37981 aacggtgcga tcagcgaagt aattatcccg cataacacca gcgctagcga actaaatgca
    38041 ccttcctgat aatccagctc ggcgcagcgc gccgtaccga gggcgtgcga ggcagtcccc
    38101 attgccagac cgcgcgcagc tttggtacga atacgcatcg cattaagcaa tgtatggcca
    38161 aataccgcgc cgaggatgcc gacgaaaatc acgcaaactg cgctgattgc cggaataccg
    38221 ccaatactgc cgccaaccgc cattgcaata ggcgtggtga ctgatttcgg caggattgac
    38281 gcggcgattt ccggtgaagc gcccatcaac aatgccacgg aagtccccgt caccatcgca
    38341 accacgctgc cgataaaaca gatggtgatg atcgatttcc agcgcgcgcg gatctggtgt
    38401 agctgctcat ataaaggata ggccagcgcg acgaccgccg gttgcagcag atcgtttagc
    38461 acttcgctac ctttaaagta gctgtcgtaa gagatgccgg tcagcattaa aaaaggaatg
    38521 atcaccacca ttgctaccag caacgggtta agcaggggaa acttataccg tgccgccagt
    38581 ttgcgggcgg caaaaaagac aatcaacgtc agcggtaatg accaccagat attcgccatc
    38641 attcttctga tcctttctga cctacgactt tacgttcacc gtgtaccagt tgcgaactcc
    38701 agctcaccac cagaaaaacc accagcgtac tgattgcaca ggaaaccact accgggccaa
    38761 actgtgcgcg gagcaaatca aaatattgca tgacgcctac gccaatcggc acaaacaata
    38821 gcgccatata gcgaatcagt acgtagcacc ccggattgac ccatttcgcc ggaagaattt
    38881 gcaaggccag caggacaaac aggatcagca tcccgatgat gctgcccgga atggttaccg
    38941 gtagcaggga agcaatgaaa atgcctgcat acaggcaggc ataaatcagg acgaaagcgc
    39001 gtaaatattg ccagataatg ttcagtgtct tactcataat caacacccca gatgagatgg
    39061 attcatcata caattaattc gtaaaacgtg ctaccgatca cattaagaaa tcagcgctta
    39121 ttttttcgct ccccggtatt acgcaatgag cagcgaactc aggcatacat caagcataga
    39181 gagggattgc agtttgcagc aatccctctt tgcagacctt taatccgttg accataggcg
    39241 ctaacatagc gcttcatttt tttgcgggtg atgatatgcg tgtgttactg gcaccgatgg
    39301 agggagtgct tgactctctg gtgcgtgaat tgctgaccga agttaacgac tacgatctgt
    39361 gcatcaccga gtttgtccgc gtggtggatc aactgctgcc ggtaaaagtc tttcatcgca
    39421 tttgccctga gctacaaaac gccagccgga caccatctgg tacgctggtg cgcgtgcagt
    39481 tgttaggtca gttcccacaa tggctggcag agaacgccgc ccgtgcggtg gagttaggtt
    39541 cctggggcgt ggatctcaat tgcggctgcc cgtcgaaaac ggtcaacggt agcggtggtg
    39601 gggcgacgtt actcaaagat cctgaactca tctaccaggg tgcaaaagcg atgcgtgaag
    39661 ctgtaccggc gcatttgccc gtcagcgtga aagtgcgtct gggctgggac agcggtgaga
    39721 agaaatttga aatcgccgat gcggttcaac aggctggtgc tacggagctg gtggtgcatg
    39781 gacggacaaa agagcagggt taccgcgcgg agcatattga ctggcaggcg attggcgaga
    39841 ttcgccagcg gctgaatatt ccggtgattg ccaacggtga aatctgggac tggcagagtg
    39901 cgcaacaatg tatggcgatc agcggctgcg acgcagtgat gattggtcgc agggcgctca
    39961 atattcccaa cctgagccgg gtggtaaaat ataacgaacc gcgaatgccg tggccggagg
    40021 tggttgcttt gctgcaaaaa tatacccgtc tggaaaagca gggcgatacc gggttatatc
    40081 acgttgcgcg gattaaacag tggttgagtt atttgcgtaa agaatacgat gaagcaacgg
    40141 aattatttca gcatgttcgg gtgttgaata attcccctga tattgcaagg gctattcagg
    40201 caattgatat cgagaaactc taaaaaacca tagtgatgtt ttttgacaat gtgttagctc
    40261 tttatcaata ggccgcacgg ttgtttggtg gcgtcaaaat tgccg
//
LOCUS       DAZFQD010000030        38507 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.030, whole genome shotgun sequence.
ACCESSION   DAZFQD010000030 DAZFQD010000000
VERSION     DAZFQD010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 38507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38507
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.030"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(14..226)
                     /gene="rpmE"
                     /locus_tag="ACHKTW_001638"
     CDS             complement(14..226)
                     /gene="rpmE"
                     /locus_tag="ACHKTW_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HFO6462686.1"
                     /translation="MKKDIHPKYEEITASCSCGNVMKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            429..2627
                     /gene="priA"
                     /locus_tag="ACHKTW_001639"
     CDS             429..2627
                     /gene="priA"
                     /locus_tag="ACHKTW_001639"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFO6462687.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRVRVPFGKQQERIG
                     VVVSVSDVSELPLNELKAVVEVLDVEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPMFPGVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIISGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            2783..3808
                     /gene="cytR"
                     /locus_tag="ACHKTW_001640"
     CDS             2783..3808
                     /gene="cytR"
                     /locus_tag="ACHKTW_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HFO6462688.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKVAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            3900..4859
                     /gene="ftsN"
                     /locus_tag="ACHKTW_001641"
     CDS             3900..4859
                     /gene="ftsN"
                     /locus_tag="ACHKTW_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HFO6462689.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            4952..5482
                     /gene="hslV"
                     /locus_tag="ACHKTW_001642"
     CDS             4952..5482
                     /gene="hslV"
                     /locus_tag="ACHKTW_001642"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HFO6462690.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            5492..6823
                     /gene="hslU"
                     /locus_tag="ACHKTW_001643"
     CDS             5492..6823
                     /gene="hslU"
                     /locus_tag="ACHKTW_001643"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HFO6462691.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQTIIIDADYVSKHLDALVADEDLSRFIL"
     gene            6890..7816
                     /gene="menA"
                     /locus_tag="ACHKTW_001644"
     CDS             6890..7816
                     /gene="menA"
                     /locus_tag="ACHKTW_001644"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HFO6462692.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQEMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            7909..8394
                     /gene="rraA"
                     /locus_tag="ACHKTW_001645"
     CDS             7909..8394
                     /gene="rraA"
                     /locus_tag="ACHKTW_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008428 - ribonuclease inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0051252 - regulation of RNA metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HFO6462693.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(8479..8724)
                     /gene="zapB"
                     /locus_tag="ACHKTW_001646"
     CDS             complement(8479..8724)
                     /gene="zapB"
                     /locus_tag="ACHKTW_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HFO6462694.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            9149..9994
                     /gene="glpF"
                     /locus_tag="ACHKTW_001647"
     CDS             9149..9994
                     /gene="glpF"
                     /locus_tag="ACHKTW_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
                     /protein_id="HFO6462695.1"
                     /translation="MSQTSTLKGQCIAEFLGTGLLIFFGVGCVAALKVAGASFGQWEI
                     SVIWGLGVAMAIYLTAGVSGAHLNPAVTIALWLFACFDKRKVIPFIVSQVAGAFCAAA
                     LVYGLYYNLFFDFEQTHHIVRGSVESVDLAGTFSTYPNPHINFVQAFAVEMVITAILM
                     GLILALTDDGNGVPRGPLAPLLIGLLIAVIGASMGPLTGFAMNPARDFGPKVFAWLAG
                     WGNVAFTGGRDIPYFLVPLFGPIVGAIVGAFAYRKLIGRHLPCDICVVEEKETTTPSE
                     QKASL"
     gene            10017..11525
                     /gene="glpK"
                     /locus_tag="ACHKTW_001648"
     CDS             10017..11525
                     /gene="glpK"
                     /locus_tag="ACHKTW_001648"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFO6462696.1"
                     /translation="MTEKKYIVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPG
                     WVEHDPMEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAI
                     VWQCRRTAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSRERARRGE
                     LLFGTVDTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEV
                     RRSSEVYGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNT
                     GEKAVKSENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFA
                     TKVQNTNGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDV
                     LEAMQADSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGL
                     AVGFWQNLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            11660..12670
                     /gene="glpX"
                     /locus_tag="ACHKTW_001649"
     CDS             11660..12670
                     /gene="glpX"
                     /locus_tag="ACHKTW_001649"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HFO6462697.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            12767..13513
                     /gene="fpr"
                     /locus_tag="ACHKTW_001650"
     CDS             12767..13513
                     /gene="fpr"
                     /locus_tag="ACHKTW_001650"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HFO6462698.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(13518..13946)
                     /gene="uspD"
                     /locus_tag="ACHKTW_001651"
     CDS             complement(13518..13946)
                     /gene="uspD"
                     /locus_tag="ACHKTW_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HFO6462699.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKMSADLLIVPFIDK"
     gene            complement(13973..14272)
                     /gene="yiiS"
                     /locus_tag="ACHKTW_001652"
     CDS             complement(13973..14272)
                     /gene="yiiS"
                     /locus_tag="ACHKTW_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HFO6462700.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(14484..14924)
                     /gene="yiiR"
                     /locus_tag="ACHKTW_001653"
     CDS             complement(14484..14924)
                     /gene="yiiR"
                     /locus_tag="ACHKTW_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFO6462701.1"
                     /translation="MTIQQWLFSFKGRIGRRDFWIWIGLWFAGMLVLFSLAGKNLLDI
                     QTAAFCLVCLLWPTAAVTVKRLHDRGRSGAWAFLMIVAWMLLAGNWAILPGVWQWAVG
                     RFVPTLILVMMLIDLGAFVGTQGENKYGKDTQDVKYKADNKSSN"
     gene            15025..15624
                     /gene="yiiQ"
                     /locus_tag="ACHKTW_001654"
     CDS             15025..15624
                     /gene="yiiQ"
                     /locus_tag="ACHKTW_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HFO6462702.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            15732..16499
                     /gene="tpiA"
                     /locus_tag="ACHKTW_001655"
     CDS             15732..16499
                     /gene="tpiA"
                     /locus_tag="ACHKTW_001655"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFO6462703.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(16554..17309)
                     /gene="cdh"
                     /locus_tag="ACHKTW_001656"
     CDS             complement(16554..17309)
                     /gene="cdh"
                     /locus_tag="ACHKTW_001656"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008715 - CDP-diacylglycerol diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HFO6462704.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVREQLDNNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHECEILR"
     gene            complement(17416..18405)
                     /gene="sbp"
                     /locus_tag="ACHKTW_001657"
     CDS             complement(17416..18405)
                     /gene="sbp"
                     /locus_tag="ACHKTW_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HFO6462705.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(18725..19687)
                     /gene="pfkA"
                     /locus_tag="ACHKTW_001658"
     CDS             complement(18725..19687)
                     /gene="pfkA"
                     /locus_tag="ACHKTW_001658"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFO6462706.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(19868..20770)
                     /gene="fieF"
                     /locus_tag="ACHKTW_001659"
     CDS             complement(19868..20770)
                     /gene="fieF"
                     /locus_tag="ACHKTW_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312867.1"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HFO6462707.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLISPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(20919..21419)
                     /gene="cpxP"
                     /locus_tag="ACHKTW_001660"
     CDS             complement(20919..21419)
                     /gene="cpxP"
                     /locus_tag="ACHKTW_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HFO6462708.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            21569..22267
                     /gene="cpxR"
                     /locus_tag="ACHKTW_001661"
     CDS             21569..22267
                     /gene="cpxR"
                     /locus_tag="ACHKTW_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HFO6462709.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            22264..23637
                     /gene="cpxA"
                     /locus_tag="ACHKTW_001662"
     CDS             22264..23637
                     /gene="cpxA"
                     /locus_tag="ACHKTW_001662"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HFO6462710.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(23743..24417)
                     /gene="yiiM"
                     /locus_tag="ACHKTW_001663"
     CDS             complement(23743..24417)
                     /gene="yiiM"
                     /locus_tag="ACHKTW_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-hydroxyaminopurine reductase"
                     /protein_id="HFO6462711.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWAREFPEQAELFVAPAFGENLSTDGLTESNVYMGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
     gene            complement(24566..25549)
                     /gene="kdgT"
                     /locus_tag="ACHKTW_001664"
     CDS             complement(24566..25549)
                     /gene="kdgT"
                     /locus_tag="ACHKTW_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015649 - 2-keto-3-deoxygluconate:proton
                     symporter activity [Evidence IEA];
                     GO_process: GO:0046411 - 2-keto-3-deoxygluconate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HFO6462712.1"
                     /translation="MQIKRSIEKISGGMMLVPLFLGALCHTFSPGAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGT
                     AGIASFEPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTV
                     IAQTGLLGILLGVAVIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEM
                     VPAFKPMAPAATSLVATAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
     gene            complement(25809..26429)
                     /gene="sodA"
                     /locus_tag="ACHKTW_001665"
     CDS             complement(25809..26429)
                     /gene="sodA"
                     /locus_tag="ACHKTW_001665"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HFO6462713.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            26714..27748
                     /gene="rhaT"
                     /locus_tag="ACHKTW_001666"
                     /pseudo
     CDS             26714..27748
                     /gene="rhaT"
                     /locus_tag="ACHKTW_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015153 - rhamnose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008645 - hexose transmembrane transport
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
     gene            complement(27745..28593)
                     /gene="rhaR"
                     /locus_tag="ACHKTW_001667"
     CDS             complement(27745..28593)
                     /gene="rhaR"
                     /locus_tag="ACHKTW_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HFO6462714.1"
                     /translation="MAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDFCELVIV
                     WRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKLNLDWQG
                     AIPGFSASAGQPHWRLGSVGMAQARQVIGQLEHESSQHVSFANEMAELLFGQLVMLLN
                     RHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQFRQQTG
                     MTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMTPSQWRH
                     LNSQKD"
     gene            complement(28667..29503)
                     /gene="rhaS"
                     /locus_tag="ACHKTW_001668"
     CDS             complement(28667..29503)
                     /gene="rhaS"
                     /locus_tag="ACHKTW_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HFO6462715.1"
                     /translation="MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLVWLEDHFADEVNWDAVAEQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            29791..31260
                     /gene="rhaB"
                     /locus_tag="ACHKTW_001669"
     CDS             29791..31260
                     /gene="rhaB"
                     /locus_tag="ACHKTW_001669"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HFO6462716.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWDVDSLESAIRLGLNMVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRTNGLMAQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASALIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPDEMCSEIQAACRETAQPIPGSDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNALLNQLCADACGIRVIAGPVEASTLGNIGIQLMTLDELNNVDDF
                     RQVVSTTANLTTFIPNPDSEIAHYLAQIHSTRQTKELCA"
     gene            31257..32516
                     /gene="rhaA"
                     /locus_tag="ACHKTW_001670"
     CDS             31257..32516
                     /gene="rhaA"
                     /locus_tag="ACHKTW_001670"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HFO6462717.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRTDLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHADDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLNP
                     AHHIDAVESKLFGIGAESYTVGSNEFYLGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPWQAVWEMYC
                     QRHDTPTGSEWLESVRAYEKAILSQRG"
     gene            32758..33582
                     /gene="rhaD"
                     /locus_tag="ACHKTW_001671"
     CDS             32758..33582
                     /gene="rhaD"
                     /locus_tag="ACHKTW_001671"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HFO6462718.1"
                     /translation="MQNITQSWFVQGMIKATTDAWLKGWDERNGGNLTLRLDDADIAP
                     YKDNFHAQPRYIPLSQPMPLLANTPFIVTGSGKFFRNVQLDPAANLGVVKVDSDGAGY
                     HILWGLTNEAVPTSELPAHFLSHCERIKATNGKDRVIMHCHATNLIALTYVLENDTAV
                     FTRQLWEGSTECLVVFPDGVGILPWMVPGTDEIGQATAQEMQKHSLVLWPFHGVFGSG
                     STLDETFGLIDTAEKSAQVLVKVYSMGGMKQTISREELIALGKRFGVTPLASALAL"
     gene            33592..33906
                     /gene="rhaM"
                     /locus_tag="ACHKTW_001672"
     CDS             33592..33906
                     /gene="rhaM"
                     /locus_tag="ACHKTW_001672"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HFO6462719.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGAHNYAIY
                     LDKARNLLFATVEIESEERWNAVASTDVCQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
     gene            34219..34665
                     /gene="frvA"
                     /locus_tag="ACHKTW_001673"
     CDS             34219..34665
                     /gene="frvA"
                     /locus_tag="ACHKTW_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HFO6462720.1"
                     /translation="MAALTASCIDLNIQGNGAYSILKQLATMALQNGFITDSHQFLQT
                     LLLREKMHSTGFGSGIAVPHGKSACVKQPFVLFARKAQAIDWKASDGEDVNCWICLGV
                     PQSGEEDQVKIIGTLCRKIIHKEFIHQLQQGDTDQVLALLNQTLSS"
     gene            34676..36127
                     /gene="frvB"
                     /locus_tag="ACHKTW_001674"
     CDS             34676..36127
                     /gene="frvB"
                     /locus_tag="ACHKTW_001674"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIBC"
                     /protein_id="HFO6462721.1"
                     /translation="MESSLRIVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQG
                     SSGVENRLSSEEIAAADYVILATGRGLSGDDRARFAGKKVYEIAISQALKNIDQIFSE
                     LPTNSHLFAADSGVKLGKQEVQSGSVMSHLMAGVSAALPFVIGGGILVALANMLVQFG
                     LPYTDMSKGAPSFTWVVESIGYLGFTFMIPIMGAYIASSIADKPAFAPAFLVCYLAND
                     KALLGTQSGAGFLGAVVLGLAIGYFVFWFRKVRLGKALQPLLGSMLIPFVTLLVFGVL
                     TYYVIGPVMSDLMGGLLHFLNTIPPSMKFAAAFLVGAMLAFDMGGPINKTAWFFCFSL
                     LEKHIYDWYAIVGVVALMPPVAAGLATFIAPKLFTRQEKEAASSAIVVGATVATEPAI
                     PYVLAAPLPMITANTLAGGITGVLVIAFGIKRLAPGLGIFDPLIGLMSPVGSFYLVLA
                     IGLALNISFIIVLKGLWLRRKAKAAQQELVHEH"
     gene            36117..37187
                     /gene="frvX"
                     /locus_tag="ACHKTW_001675"
     CDS             36117..37187
                     /gene="frvX"
                     /locus_tag="ACHKTW_001675"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFO6462722.1"
                     /translation="MNIELLQQLCEASAVSGDEQEVRDILINTLEPCVNEITFDGLGS
                     FVARKGNKGPKVAVVGHMDEVGFMVTHIDESGFLRFTTIGGWWNQSMLNHRVTIRTHK
                     GFKIPGVIGSVAPHALTEKQKQQPLSFDEMFIDIGANSRDEVEKRGVAMGDFISPEAN
                     FACWGEDKVVGKALDNRIGCAMMAELLQTVNNPEITLYGVGSVEEEVGLRGAQTSAEH
                     IKPDVVIVLDTAVAGDVPGIDNIKYPLKLGQGPGLMLFDKRYFPNQKLVAAFKNCATQ
                     NDLPLQFSTMKTGATDGGRYNVMGGGRPVVALCLPTRYLHANSGMISKADYDALLTLI
                     RGFLTTLTAEKVNAFSQFRQVD"
     gene            37187..>38507
                     /locus_tag="ACHKTW_001676"
     CDS             37187..>38507
                     /locus_tag="ACHKTW_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative frv operon regulatory protein"
                     /protein_id="HFO6462723.1"
                     /translation="MLNERQLKIVDLLEQQPRTPGELAQQTGVSGRTILRDIDYLNFT
                     LNGKARISASGSAGYQLEIFERRSFFQLLQKHDNDDRLLALLLLNTFTPRAQLASALN
                     LPETWVAERLPRLKQRYERACCLASRPGLGHFIDETEEKRVILLANLLRKDPFLIPLA
                     GVTRDNLQHLSTACDNQHRWPLMQGDYLSSLILAIYALRNQLTDEWPQYPGDEIKQIV
                     EHSGLFLGDNAVRTLTGLIEKQHQQAQIISADNVQRLLQRVPGIASLNIIDTQLVENI
                     TGHLLRCLAAPVWIAEHRQSSMNNLKAAWPAAFDMSLHFITLLREQLDIPLFDSDLIG
                     LYFACALERHQNERQPIILLSDQNAIATINQLAIERDVLHCRVIIARSLSELVAIREE
                     IEPLLIINNSHYLLDDAVNNYITVKNIITAAGIEQIKHFLATAFIRQQ"
BASE COUNT         9335 a        10016 c        10020 g         9136 t
ORIGIN      
        1 cggaaatccg gtcttatttg ctgcccggga tgttgaaacg cttgttgaag cggtcaacac
       61 ggccaccggt agcaacatca cgctgtttgc cagtgaagaa cgggtggcac ttgctgcaca
      121 cgtcgaggtt caggtcatga ccaacggtgg agcggatttt cattacgtta ccgcaagagc
      181 agctagcagt aatttcttcg tatttcgggt gaatatcttt tttcatggga aaacctcggt
      241 ttaaggccgc gtcgctcttc cagccctaac gccagacacc acgcgatgtt aaaagtatag
      301 cttcaatacg atcatttcgt acgaaggcgc gaaatcatac agaaattaac cagcgtatgc
      361 aaactgatcc gcactcttct acggcaatgt gtatactaac ccaccgaatt tcaagtcagg
      421 atgatgctat gcccgttgcc cacgttgcct tgcccgttcc gcttcctcgt acctttgact
      481 atctgctgcc agaaggcatg acggttaaag ccgggtgtcg cgtgcgcgtg ccgtttggca
      541 aacagcagga gcgcatcggg gttgtggtgt cagttagcga tgtcagtgag ctgccgctta
      601 acgagctgaa agcggtagtg gaagtgctgg acgttgagcc ggtatttact cactccgtct
      661 ggcggttact gctctgggcg gcagattact atcatcatcc gattggcgat gtgctgtttc
      721 atgccttgcc gattttactg cgccaggggc ggcctgcggc gaacgcgccg atgtggtact
      781 ggtttgccac tgaacaaggc caggcggtgg atctgaacag cctgaaacgc tcccccaagc
      841 aacaacaggc gctggcggcg ttacggcaag gcaaaatctg gcgcgaccag gtcgccacgc
      901 tcgaatttaa tgatgccgcg ttgcaggcgc tacgcaaaaa aggtctgtgt gatttagcaa
      961 gtgaaacacc agagtttagc gactggcgaa cgaactatgc cgtttctggt gagcggttgc
     1021 gattgaatac cgaacaagcc accgccgttg gcgcaattca tagcgcggca gatacttttt
     1081 ctgcctggct gctggcgggc gttaccggtt ccggtaaaac ggaggtttat ctcagtgtac
     1141 tggaaaacgt gctcgctcag ggcaaacagg cgctggtgat ggtgccggaa atcggcctga
     1201 caccgcaaac tatcgcccgt tttcgtgaac gttttaatgc ccccgtggaa gttctgcatt
     1261 ccggcctgaa cgacagcgag cggctttcgg cgtggttgaa agcgaaaaat ggcgaggcgg
     1321 cgattgtgat cggcacccgc tccgcgctgt ttacgccgtt taaaaatctc ggcgtgattg
     1381 tcattgatga agagcacgac agctcctaca agcagcagga aggctggcgc tatcatgccc
     1441 gcgacctggc ggtgtatcgt gcgcatagcg agcaaatccc gattattctt ggctccgcaa
     1501 cgcccgcgct ggaaacgtta tgcaacgtcc agcagaaaaa ataccgcctg ctgcgcctga
     1561 cccgtcgggc agggaatgcg cgtccggcaa ttcaacatgt gctggattta aaaggtcaga
     1621 aggtgcaggc aggtctggct ccggcgttaa tcacccgtat gcgccagcat ttacaggcca
     1681 acaatcaggt tatcctcttt cttaatcgtc ggggttttgc gcctgcgctg ctgtgccacg
     1741 actgcggctg gatagccgaa tgcccacgtt gcgatcacta ctacacgctg catcaggcgc
     1801 aacagcattt gcgctgtcac cactgtgaca gtcagcgtcc ggtgccgcgc cagtgccctt
     1861 cctgcggttc cacgcacctg gtccccgtgg ggctgggcac cgaacagctt gaacagacgc
     1921 ttgcgccgat gttccccggc gtgcccattt cacgtatcga ccgcgatacc accagccgca
     1981 aaggggcgct ggaacagcaa ctggcggaag tgcatcgcgg cggcgcgagg atcttgattg
     2041 gcacgcaaat gctggcgaaa ggtcaccact ttccggatgt gacgctggtg gcgttactgg
     2101 atgtggacgg cgcgctgttt tctgccgatt ttcgctcggc agaacgtttc gcccagcttt
     2161 acacccaggt cgccggtcgt gccgggcgtg cgggtaaaca gggcgaagtg gtgctgcaaa
     2221 cgcaccatcc ggaacatcct ctgttgcaaa cgttgctcta taaaggctac gacgcctttg
     2281 ccgaacaggc gctggctgag cgacgcatga tgcagctgcc gccgtggacc agccatgtga
     2341 ttgtgcgtgc ggaagatcat aacaatcagc atgcgccgct gttcctgcaa caactgcgta
     2401 atctgatcct ttccagcccg ctggcagacg acaaactgtg ggttctcggc ccggttccgg
     2461 ctctggcacc taagcgtggt ggccgctggc gctggcagat cttgttgcag cacccttccc
     2521 gcgtgcgctt gcaacacatc attagtggta cgctggcgct tattaataca ataccggatt
     2581 cccgcaaggt gaaatgggtg ttggatgtcg atcccatcga aggctaatgc gcaaacgttg
     2641 cgaggcggat cgaaaaattc aatattcatc acacttttca tgaaaattct gtaaccgttt
     2701 tcacgcgcta tctgctaaaa atgttgccga tgtgaagtaa acatggatgt agtacgcctg
     2761 acgtgccagg cgaggagtga gtgtgaaagc gaagaagcag gaaactgccg cgaccatgaa
     2821 agacgttgcc ctcaaggcaa aagtctctac agcgactgtc tcccgagcat taatgaatcc
     2881 cgataaagtc tcccaggcca cccgtaatcg ggttgaaaaa gtggcccggg aagtgggtta
     2941 tttaccgcaa cctatggggc gcaacgtcaa gcgtaatgaa tcccgcacca ttctggtgat
     3001 tgtcccggat atctgcgatc ccttctttag cgaaattatt cgcggtatcg aagttacggc
     3061 ggcaaatcac ggatatctgg tgctgattgg cgactgtgcg catcaaaatc agcaggaaaa
     3121 aacctttatc gatttgatca tcaccaagca aattgatggc atgttgctgc tgggctcaag
     3181 gctgccgttt gatgccagca ttgaggaaca gcgtaatctg ccgccgatgg tgatggcgaa
     3241 cgaatttgca ccggaactgg agctgcctac cgttcatatc gacaatctga ccgccgcatt
     3301 tgatgcagta aattatttat atgagcaagg gcataaacgg attggctgta tagccggtcc
     3361 cgaagagatg ccactgtgtc actaccgcct gcaaggctat gttcaggcgc tgcgtcgctg
     3421 cggcattatg gttgatccgc aatacatcgc ccgtggcgac ttcaccttcg aagccggaag
     3481 caaagcgatg cagcagctgc ttgatcttcc acaaccgcct actgctgtct tctgccatag
     3541 cgatgtgatg gcgctcggcg cactttctca ggcaaaacgc caggggctga aagtcccgga
     3601 agacctttcc ataatcggtt ttgataacat cgacctgacg caattttgtg atccgccgct
     3661 gacaaccatc gcgcagccgc gttacgaaat cggtcgggaa gctatgctgt tattgcttga
     3721 tcaaatgcag gggcaacacg ttggcagtgg ctctcgttta atggactgcg aacttatcat
     3781 ccggggatca acacgcgcgt taccttaaag taaaccttta agactgccgt gactggtcaa
     3841 aggcccgccg cttaagtaac atggcgggct gacgaacgaa taaatacagc gaaacgatag
     3901 tggcacaacg agattatgta cgccgcagcc aaccggcacc ttcgcggcga aaaaagagca
     3961 cctcacggaa aaagcaacga aatctgcctg cggtttctcc cgctatggtc gctattgctg
     4021 ccgccgttct tgtgaccttt atcggtggtc tgtacttcat tacgcatcac aagaaagaag
     4081 agtccgagac gctgcaaagc cagaaagtga ccggtaacgg actaccacca aaaccagaag
     4141 aacgctggcg ctacattaaa gagctggaaa gtcgccagcc gggtgtgcgt gcgcccacag
     4201 aaccttctgc cggtggtgaa gtgaaaacgc cggagcaact gacaccagaa caacgtcagc
     4261 ttcttgaaca aatgcaggct gatatgcgcc agcagccaac gcagctggtt gaagttccgt
     4321 ggaatgaaca gacgccggaa cagcgtcagc aaacgctaca acgccagcgt caggcgcagc
     4381 aattagcgga gcagcaacgc ctggctcagc agtcgcgtac gactgaacaa agctggcagc
     4441 agcagacgcg tacgtcgcaa gccgcgccgg tacaggcgca accgcgccag tctaaaccgg
     4501 cttctactca gcagccgtac caggatctgc tgcaaacccc tgcgcacacg accgcgcagt
     4561 cgaagccgca gcaagctgcg ccggttgctc gtgccgctga cgcgccaaaa ccgacggcgg
     4621 agaaaaaaga cgaacgccgc tggatggtgc agtgcggttc gttcagaggc gcggaacagg
     4681 cagagacggt acgtgctcag ctggcgttcg aaggatttga ctcgaaaatc accaccaaca
     4741 atggctggaa tcgtgtggtc attggcccgg tgaaaggcaa agagaacgca gacagcaccc
     4801 tcaatcggtt gaagatggcg ggtcatacaa actgcattcg gctcgccgcc gggggttgaa
     4861 accctcaaaa tcccccccat ctataattgc attatgcccc gtacttttgt acggggtttg
     4921 tactctgtat tcgtaaccaa ggggtcagct cgtgacaact atagtaagcg tacgccgtaa
     4981 cggccatgtg gtcatcgctg gtgatggtca ggccacgttg ggcaataccg taatgaaagg
     5041 caacgtgaaa aaggtccgcc gtctgtacaa cgacaaagtc atcgcgggct ttgcgggcgg
     5101 tactgcggat gcttttacgc tgttcgaact gtttgaacgt aaactggaaa tgcatcaggg
     5161 ccatctggtc aaagccgccg ttgagctggc gaaagactgg cgtaccgatc gcatgctgcg
     5221 caaacttgaa gcactgctgg cagtcgcgga tgaaactgca tcgcttatca tcaccggtaa
     5281 cggtgacgtg gtgcagccag aaaacgatct tattgctatc ggctccggcg gcccttacgc
     5341 ccaggctgcg gcgcgtgcac tgttagaaaa tactgaactg agtgctcgtg aaattgctga
     5401 aaaggcgttg gatattgcag gcgacatttg catctatacc aaccatttcc acaccatcga
     5461 agaattaagc tacaaagcgt aaggatctcc catgtctgaa atgaccccac gcgaaatcgt
     5521 cagcgaactg gataagcaca tcatcggcca ggacaacgcc aagcgttctg tggcgattgc
     5581 tctgcgtaac cgctggcgtc gcatgcagct caacgaagag ctgcgccatg aagtgacccc
     5641 gaaaaatatc ctgatgatcg gcccgaccgg tgtcggtaaa actgaaatcg cccgtcgtct
     5701 ggctaagctg gcgaatgcgc cgttcatcaa agttgaagcg accaaattca ccgaagtggg
     5761 ctacgtcggt aaggaagtgg attctattat tcgcgatctg accgatgccg ccgtgaaaat
     5821 ggtacgcgtc caggctatcg agaaaaaccg ttatcgcgct gaagaactgg cagaagaacg
     5881 tattctcgac gtgctgatcc cacctgctaa aaacaactgg ggacagaccg aacagcagca
     5941 ggaaccgtcc gctgctcgtc aggcattccg caaaaaactg cgtgaaggcc agcttgatga
     6001 caaagaaatc gagatcgatc ttgccgcagc accgatgggc gttgaaatta tggctcctcc
     6061 aggcatggaa gagatgacca gccagctgca gtccatgttc cagaacctgg gcggccagaa
     6121 gcaaaaagcg cgtaagctga aaatcaaaga cgccatgaag ctgctgattg aagaagaagc
     6181 ggcgaaactg gtgaacccgg aagagctgaa gcaagacgct atcgacgctg ttgagcagca
     6241 cgggatcgtg tttatcgacg aaatcgacaa aatctgtaag cgcggcgagt cttccggtcc
     6301 ggatgtttct cgtgaaggcg ttcagcgtga cctgctgccg ctggtagaag gttgcaccgt
     6361 ttccaccaaa cacgggatgg tcaaaactga ccacattctg tttatcgctt ctggcgcgtt
     6421 ccagattgcg aaaccgtctg acctgatccc ggaactgcaa ggtcgtctgc caatccgcgt
     6481 tgaactgcag gcgctgacca ccagcgactt cgagcgtatt ctgaccgagc cgaatgcctc
     6541 tatcaccgtg cagtacaaag cactgatggc gactgaaggc gtaaatatcg agtttaccga
     6601 ctccggtatt aagcgcatcg cggaagcggc atggcaggtg aacgaatcta ccgaaaacat
     6661 cggtgctcgt cgtttacaca ctgttctgga gcgtttaatg gaagagattt cctacgacgc
     6721 cagcgattta agcggtcaaa ctatcatcat tgacgcagat tatgtgagca aacatctgga
     6781 tgcgttggtg gcagatgaag atctgagccg ttttatccta taatcgcgtt caatcatttt
     6841 catcattgtt tgatggggct gaaaggcccc atttttattg gcgcgtatta tgactgaaca
     6901 acaaattagc cgaactcagg cgtggctgga aagtttacga cctaaaaccc tccccctcgc
     6961 ctttgctgca attatcgtcg ggacagcgct ggcatggtgg caaggtcact tcgatccgct
     7021 ggtcgccctg ctggcactga ttaccgccgg gctattacag atcctttcta acctcgccaa
     7081 tgattacggc gatgcggtaa aaggcagcga taaacctgac cgcattgggc cgctacgcgg
     7141 catgcaaaaa ggggtcatta cccagcaaga gatgaaacgg gcgctcatta ttaccgtcgt
     7201 gctcatctgt ctctccgggc tggcactggt tgcagtggca tgccatacgc tggccgattt
     7261 tgtcggtttc ctgattcttg gcgggttgtc gatcattgcc gctatcacct acaccgtggg
     7321 caatcgtcct tatggttata tcggtctggg tgatatttcc gtactggttt tctttggctg
     7381 gttgagtgtc atggggagct ggtatttaca ggctcataca ttgattccgg cactgatcct
     7441 tccggcgacc gcatgcggcc tgctggcaac ggcagtactg aatattaata acctgcgtga
     7501 tatcaatagc gaccgcgaaa atggcaaaaa cacgctggtg gtgcgcttag gtgaagtgaa
     7561 cgcgcgtcgt tatcatgcct gcctgctgat gggctcgctg gtgtgtctgg cgctgtttaa
     7621 tctcttttcg ctgcatagcc tgtggggctg gctgttcctg ctggcggcac cattactggt
     7681 gaagcaagcc cgttatgtga tgcgggaaat ggacccggtg gcgatgcgac caatgctgga
     7741 acgtactgtc aagggagcgt tactgactaa cctgctgttt gttttaggga tattcctaag
     7801 ccagtgggca gcataactga caaatatcaa ttaacaattg atgattttgc caacagccta
     7861 catagcgcga tatactgaaa attctcgcag caactgaatg ttaagcctat gaaatacgat
     7921 acttccgagc tttgtgacat ctatcaagaa gatgttaacg tcgtggaacc gctgttctcc
     7981 aactttggcg gacgggcgtc gtttggcgga caaataatca cggtaaaatg tttcgaggac
     8041 aacgggttgc tgtacgatct gctcgaacag aatggccgtg gtcgtgttct tgtcgtcgat
     8101 ggcggtggtt ctgttcgtcg cgcactggtc gatgctgaac tggcgcgtct ggcagtacaa
     8161 aatgaatggg aaggtctggt catttacggc gcggtgcgtc aggtagatga cctggaagag
     8221 ttggatatcg gcatccaggc gatggcggca attccggttg gtgccgctgg cgaaggcatt
     8281 ggcgaaagcg atgtccgcgt caattttggc ggtgtcacct tcttctccgg cgaccatctt
     8341 tatgccgaca ataccgggat tattctttct gaagatccgc tggatattga atgatagaaa
     8401 aggcaccgca aggtgccttt tttctgcgtt acctgttggc ctacacagta aagaaattac
     8461 gcggaagatg aagcgtaatc agacctcttc catgcgaccc agcagggcct gcagacgttc
     8521 ctgccagccg ttctgctgtt ctttcagatg gttgttctca cgctccagct cttcgcgctg
     8581 atgctgggca ttttgaactt cctgcgacag tgagttgttt ttttctttca gctcttcgat
     8641 ttccatctgc aacagagtga tggtatcaat cgcctgctgt acttttgctt ccagtttctc
     8701 aaacacttct aatgacattg tcatacctct cctgaattgc aaggcgttga tggataaaaa
     8761 tcctcgtccc gattaccggt gacgccttaa taaatacgag cgcactttag ttagctccga
     8821 ttgtatgaag ccgcgccatc gctgtccagc ggcacgcctt gcagattacg gtttgccaca
     8881 cttttcatcc ttctcctggt gacataatct acatcaatcg aaaatgttaa taaatttgtt
     8941 gcgcgaatga tctaacaaac atgcatcatg tacaatcaga tggaataaat ggcgcgataa
     9001 cgctcatttt atgacgaagc acacacattt taagttcgat atttctcgtt tttgctcgtt
     9061 aacgataagt ttacagcatg cctacaagca tcgtggaggt ccgtgacttt cacgcataca
     9121 acaaacatta actcttcagg atccgattat gagtcaaaca tcaaccttga aaggccagtg
     9181 cattgctgaa ttcctcggta ccgggttgtt gattttcttc ggtgtgggtt gcgttgcagc
     9241 actaaaagtc gctggtgcgt cttttggtca gtgggaaatc agtgtcattt ggggactggg
     9301 ggtggcaatg gccatctacc tgaccgcagg ggtttccggc gcgcatctta atcccgctgt
     9361 taccattgca ttgtggctgt ttgcctgttt cgacaagcgc aaagttattc cttttatcgt
     9421 ttcacaagtt gccggcgctt tctgtgctgc ggctttagtt tacgggcttt actacaattt
     9481 atttttcgac ttcgagcaga ctcatcacat tgttcgcggc agcgttgaaa gtgttgatct
     9541 ggctggcact ttctctactt accctaatcc tcatatcaat tttgtgcagg ctttcgcagt
     9601 tgagatggtg attaccgcta ttctgatggg gctgatcctg gcgttaacgg acgatggcaa
     9661 cggtgtacca cgcggccctt tggctccctt gctgattggt ctactgattg cggtcattgg
     9721 cgcatctatg ggcccattga caggttttgc catgaaccca gcgcgtgact tcggtccgaa
     9781 agtctttgcc tggctggcgg gctggggcaa tgtcgccttt accggcggca gagacattcc
     9841 ttacttcctg gtgccgcttt tcggccctat cgttggcgcg attgtaggtg catttgccta
     9901 ccgcaaactg attggtcgcc atttgccttg cgatatctgt gttgtggaag aaaaggaaac
     9961 cacaactcct tcagaacaaa aagcttcgct gtaatatgac tacgggacaa ttaaacatga
    10021 ctgaaaaaaa atatatcgtt gcgctcgacc agggcaccac cagctcccgc gcggtcgtaa
    10081 tggatcacga tgccaatatc attagcgtgt cgcagcgcga atttgagcaa atctacccaa
    10141 aaccaggttg ggtagaacac gacccaatgg aaatctgggc cacccaaagc tccacgctgg
    10201 tagaagtgct ggcgaaagcc gatatcagtt ccgatcaaat tgcagctatc ggtattacga
    10261 accagcgtga aaccactatt gtctgggaaa aagaaaccgg caagcctatc tataacgcca
    10321 ttgtctggca gtgccgtcgt accgcagaaa tctgcgagca tttaaaacgt gacggtttag
    10381 aagattatat ccgcagcaat accggtctgg tgattgaccc gtacttttct ggcaccaaag
    10441 tgaagtggat cctcgaccat gtggaaggct ctcgcgagcg tgcacgtcgt ggtgaattgc
    10501 tgtttggtac ggttgatacg tggcttatct ggaaaatgac tcagggccgt gtccatgtga
    10561 ccgattacac caacgcctct cgtaccatgt tgttcaacat ccataccctg gactgggacg
    10621 acaaaatgct ggaagtgctg gatattccgc gcgagatgct gccagaagtg cgtcgttctt
    10681 ccgaagtata cggtcagact aacattggcg gcaaaggcgg cacgcgtatt ccaatctccg
    10741 ggatcgccgg tgaccagcag gccgcgctgt ttggtcagtt gtgcgtgaaa gaagggatgg
    10801 cgaagaacac ctatggcact ggctgcttta tgctgatgaa cactggcgag aaagcggtga
    10861 aatcagaaaa cggcctgctg accaccatcg cctgcggccc gactggcgaa gtgaactatg
    10921 cgttggaagg tgcggtgttt atggcaggcg catccattca gtggctgcgc gatgagatga
    10981 agttgattaa cgacgcctac gattccgaat atttcgccac caaagtgcaa aacaccaatg
    11041 gtgtgtatgt ggttccggca tttaccgggc tgggtgcgcc gtactgggac ccgtatgcgc
    11101 gcggggcgat tttcggtctg actcgtgggg tgaacgctaa ccacattatc cgcgcgacgc
    11161 tggagtctat tgcttatcag acgcgtgacg tgctggaagc gatgcaggcc gactctggta
    11221 tccgtctgca cgccctgcgc gtggatggtg gcgcagtagc aaacaatttc ctgatgcagt
    11281 tccagtccga tattctcggc acccgcgttg agcgcccgga agtgcgcgaa gtcaccgcat
    11341 tgggtgcggc ctatctcgca ggcctggcgg ttggcttctg gcagaacctc gacgagctgc
    11401 aagagaaagc ggtgattgag cgcgagttcc gtccaggcat cgaaaccact gagcgtaatt
    11461 accgttacgc aggctggaaa aaagcggtta aacgcgcgat ggcgtgggaa gaacacgacg
    11521 aataatgtaa atgccgaatg aagcgtttat gccgcatccg gtagtcccga aacgtgcggg
    11581 ggcaaccccg cacacatcaa taatccctcc cttcccctgt gctacacttc gcgccattcc
    11641 ttactgctta gagtttgcta tgagacgaga acttgccatc gaattttccc gcgtcaccga
    11701 atcagcggcg ctggctggct acaaatggtt aggacgcggc gataaaaaca ccgcggacgg
    11761 cgcggcggta aacgccatgc gtattatgct caaccaggtc aacattgacg gcaccatcgt
    11821 cattggtgaa ggtgaaatcg acgaagcacc gatgctctac attggtgaaa aagtcggtac
    11881 tggtcgcggc gacgcggtag atattgctgt tgatccgatt gaaggcacgc gcatgacggc
    11941 gatgggccag gctaacgcgc tggcggtgct ggcagtaggc gataaaggct gcttcctcaa
    12001 tgcgccggat atgtatatgg agaagctgat tgtcgggccg ggagccaaag gcaccattga
    12061 tctgaacctg ccgctggcgg ataacctgcg caatgtagcg gcggcgctcg gcaaaccgtt
    12121 gagcgaactg acggtaacga ttctggctaa accacgccac gatgccgtta tcgctgaaat
    12181 gcagcaactc ggcgtacgcg tatttgctat tccggatggc gacgttgcgg cctcaattct
    12241 cacctgtatg ccagacagcg aagttgacgt gctgtacggt attggtggcg cgccggaagg
    12301 cgtagtttct gcggcggtga tccgcgcatt agatggcgac atgaacggtc gtctgctggc
    12361 gcgtcatgac gtcaaaggcg acaacgaaga gaatcgtcgc attggcgagc aggagctggc
    12421 acgctgcaaa gcgatgggca tcgaagccgg taaagtattg cgcctgggcg atatggcgcg
    12481 cagcgataac gtcatcttct ctgccaccgg tattaccaaa ggcgatctgc tggaaggcat
    12541 tagccgcaaa ggcaatatcg cgactaccga aacgctgctg atccgcggca agtcacgcac
    12601 cattcgccgc attcagtcca tccactatct ggatcgcaaa gacccggaaa tgcaggtgca
    12661 catcctctga ttgatttgat cgattgagcc ttccagtcct tcgggactgg aatttttttg
    12721 ttcggagaac gaagataagg caagtcaatc aaaacaggag aaaaacatgg ctgattgggt
    12781 aacaggcaaa gtcactaaag tgcagaactg gaccgacgcc ctgtttagtc tcaccgttca
    12841 cgcccccgtg cttccgttta ccgccgggca attcaccaag cttggccttg aaatcgacgg
    12901 cgaacgcgtc cagcgcgcct actcctatgt taactcgccc gataatcccg atctggagtt
    12961 ttacctggtc accgtccccg atggcaaatt aagcccacga ttggcggcac tgaaaccagg
    13021 cgatgaagtg caggtggtta gcgaagcagc tggcttcttt gttctggatg aagtaccgga
    13081 ctgcgaaacg ctatggatgc tggcaaccgg tacagcgatt ggcccttatt tatcgattct
    13141 gcaactaggc aaagatttag atcgcttcaa aaatctggtc ctggttcacg ccgcacgtta
    13201 tgccgccgac ttaagctatt tgccactgat gcaagaactg gaaaaacgct acgaagggaa
    13261 actgcgcatt cagacggtgg tcagtcggga aacggcagcg gggtcgctca ccgggcggat
    13321 accggcgtta attgaaagtg gggaactgga aagcgcgatt ggcctgccga tgaataaaga
    13381 aaccagccat gtgatgctgt gcggcaatcc acagatggtg cgcgatacac aacagttgct
    13441 gaaagagacc cggcagatga cgaaacattt acgtcgccga ccgggccata tgacagcgga
    13501 gcattactgg taagcggtta cttatcgata aacggcacga tgagcaaatc cgcactcatc
    13561 ttattgatca tcccgcgata tgccggcatc aaacggttga taaatgagtg atgatgacca
    13621 cagacaagga ggtcgcactg ctctttttgc ataatttcca gcagtgtttc cggcatttct
    13681 ccgcgttcaa tacgcagttt tgtcttcggc cattgaatat ttttcgtcag tttatacagc
    13741 ttgttatccg acttattctt caacaattga agaatatctt ctgttgcagg gaagtagata
    13801 cccggataca actcgcttaa gccatcatca atatgaatta acgtcaggtg agcgtcatta
    13861 tgtctggcga gctccagagc tttattcacc agtaaggcat cttcttcatt cccggaaatt
    13921 gccacgccaa tgtgtttata agccatgttt aactccttct aaagcaactc catcaagcta
    13981 acgaacgcag tgataactca aaaataatca tctcggcctg gcatgagaaa gtgaaggccg
    14041 catcaagctc aacttgcccc tccagatcct taatcttgta ggccacgctg gccccttcat
    14101 ctgcagagga tgcagcggcg gcaagctctt tcagtttggt cataaaagac tctgcttctt
    14161 cgcgggtggc aaaaaagcgc gaaaacttac tggtacagtt gtcgttatca atcaccgtac
    14221 cgatatcaat cgcacatcct ttagtactgc atttatctac gacatctttc atagggcacc
    14281 tctgtatatt cgcctctctg tcgattacga cttaagaata atgcgccgtt aagggaagaa
    14341 caaacaagta atgatcttat ggattaccat tttttttgat attgcttaag gtgaagagtt
    14401 cagaaggatt ttccgggtat tgtcatttgc atcgtggaaa taacaatccc ggaaaaaaat
    14461 agcagtaaga gatatattaa aggttaatta cttgatttat tgtcggcttt atacttcaca
    14521 tcctgagtat ctttaccata tttattttcg ccttgtgtgc caacaaacgc accaagatcg
    14581 ataagcatca tcaccagaat caacgtcggg acaaaacgcc ccaccgccca ttgccagaca
    14641 cccggtaaaa tcgcccagtt acccgccagc agcatccacg ccacaatcat cagaaatgcc
    14701 catgcgccgg aacgcccgcg atcatgcaag cgcttaacag ttactgccgc tgttggccaa
    14761 agcaagcaca caaggcaaaa cgccgcggtc tgaatatcga gtaaattttt acccgccagt
    14821 gagaaaagca ccagcatgcc tgcgaaccac aggcctatcc aaatccagaa atcacggcgt
    14881 ccaatacgcc ctttaaatga gaataaccat tgctgtatgg tcatgtaagt tccttgatgg
    14941 ttgtcttttc caggattcta cccttttgac aagggcgaca gatatcgttt taatcggagc
    15001 cagtcataac aaaaggtact gtcaatgaag ccagggtgta cgctgttttt tctcttatgt
    15061 tctgcattaa ccgttacaac aacggcgcat gcacaaacac cagatacggc aacgaccgcg
    15121 ccttatctgc tggctggagc ccctactttc gatctctcca tcagccagtt tcgagaagac
    15181 tttaacagcc agaatcctag cctgccactg aacgaatttc gtgccatcga cagcagtccc
    15241 gacaaagcca atctcactcg tgctgcaagt aaaattaacg agaacttgta tgcttctaca
    15301 gcgctggagc gcggtacctt aaaaatcaaa agcattcaaa tgacctggct acccatccag
    15361 gggccagagc aaaaagcagc gaaagcgaaa gctcaggaat acatggcagc agtgatccgc
    15421 acactcaccc cattaatgac caaaacacaa agccagaaaa aactgcagtc gctgctaacg
    15481 gcggggaaaa acaaacgtta ttacaccgag acagaaggtg cactgcgtta tgttgtcgcg
    15541 gacaacggcg aaaaggggct gaccttcgct gttgaaccga ttaagctggc gctatctgaa
    15601 tcgcttgaag gtttgaataa atgacaaaaa gcaaagcctt tgtgccgatg aatctctata
    15661 ctgtttcaca gacctgctgc cctgcggggc ggccatcttc ctttattcgc ttataagcgt
    15721 ggagaattaa aatgcgacat cctttagtga tgggtaactg gaaactgaac ggcagccgcc
    15781 acatggttca cgagctggtt tctaacctgc gtaaagagct ggcaggtgtt gctggctgtg
    15841 cggttgcaat cgcaccaccg gaaatgtaca tcgatatggc gaagcgcgaa gctgaaggca
    15901 gccacatcat gctgggtgcg caaaacgtgg acctgaacct gtccggcgca ttcaccggtg
    15961 aaacttctgc cgctatgctg aaagacatcg gcgctcagta catcatcatc ggccactctg
    16021 aacgtcgtac ttaccacaaa gagtctgacg aactgatcgc gaaaaaattc gcggtgctga
    16081 aagagcaggg cctgactccg gttctgtgca tcggtgaaac cgaagctgaa aacgaagcgg
    16141 gcaaaactga agaagtttgc gcacgtcaga tcgacgcggt actgaaaact cagggtgctg
    16201 cggcattcga aggtgcggtt atcgcttacg aacctgtatg ggcaatcggt actggcaaat
    16261 ctgcaactcc ggctcaggca caggctgttc acaaattcat ccgtgaccac atcgctaaag
    16321 ttgacgctaa catcgctgaa caagtgatca ttcagtacgg cggctctgta aacgcgtcta
    16381 acgctgcaga actgtttgct cagccagata tcgacggcgc gctggttggc ggtgcttctc
    16441 tgaaagctga cgctttcgca gtgatcgtta aagctgcaga agcggctaaa caggcttaag
    16501 tctgacaggt gccggatttc atatccggca cttactttcc ttaactcctc gccttaacgc
    16561 aaaatctcac actcatgatc ctgaatttcc tcggctgaag cacggttaag cgtcagtaga
    16621 tttcgttgtg tcgccagcaa tacaaatgag ttatcactct gccgtaccat cgccagcccg
    16681 tagcttccca tatgttcccg cgcctcaggt acttcttctg ccagcatcat aaatgggctg
    16741 cgttgtacca gttcgctttc cgttacccga cgcgccaggt attcatgccc gcgcaaacca
    16801 cctggcagtg gcaaccagcg gctgctgatg ttcgccagat tgttatccag ttgttcgcgc
    16861 acatcaggac gaatacaaga gatatgaata tgaaaatggt tttgcgtacg cccggtgcgg
    16921 gagttgatcg ccaaagaaac cgcgcgatcg ggaaccggct ggccgtattt tttgctcata
    16981 aaatcacgcg cctgccaggc caaccaaaag aagttcggcg ttgaaggatc ggtcaacaaa
    17041 ggactttcag taccgttaat acgatacgtt ggcatcaaca gatattgcag tggaccatta
    17101 agatctttta aaaccacgta tccggcattg ggtttgactt ccgcacatgg cgaaggattt
    17161 tgattttgct gctgattggg caaacattcc tcaaggacaa tcttacgtaa tgtatccgac
    17221 tcttcaccgg ttaatttcca gtaaccaata ccggcagcca caacggcgat aactatcatc
    17281 accaaaaaaa gaagacccgc ttttttcatc tttttttccc tgtacctcaa agagatgcaa
    17341 gggtaacgca aaatcgtgac aaataaaaaa cccggtcgcg agaaaccacc gggtttgata
    17401 attatcctgg ggaaatcagc gtttgctgat ctgatcgaac gtaccgccgt tagcaaaatg
    17461 ctctttttgc gctttcgtcc agccgccgaa ctcttcatca atggtgaata acttcagctt
    17521 tggaaacgca ttttcgtact ttttcgccac ctcagcgtcg cgcggacggt agtagttttt
    17581 cgcggcaatt tcctgacctt ctggcgagta gagatatttc aggtaggctt ccgccacctc
    17641 tttggtgcct tttttctcga ccactttatc gaccaccgac acggttggct ctgcgaggat
    17701 agactcactc ggcgtgacga tttcgaattt atctttcccc agttcattcg ctgccagcag
    17761 agcttcgttt tcccaggcga tcagtacatc gccaatcccg cgctcgacaa aggtgttagt
    17821 ggagccgcgc gcgccagaat ccagaacttc gacgttttta tacagtgccc gaacaaaatc
    17881 ctgtgctttt gcctgatcgt tgttgttgtg atgcagcgcg tagccccagg cagccaggta
    17941 gttccagcgt gcgccaccag agcttttcgg attaggcgtg atcaccgaaa cacccggttt
    18001 aatcagatcg ttccagtcat ggatctgctt cggattgccc ttacgcacca ggaaaacaat
    18061 ggtggaagtg tacggtgcgg agttatccgg cagacgtttg atccactctt tatcaatccg
    18121 cccgcgttcc gcaattgcgt ccacgtcata ggccagagcc agcgtgacaa catcagcttc
    18181 aataccgttg attaccgacg tcgcttgttt acctgaacca ccgtgtgact gacgaatcac
    18241 cacgttatca ccagtttgct gtttccagtg ggcgctgaat gccttgttgt actgttcgta
    18301 caattcgcgc gttggatcat atgaaacgtt aagaagctga atatcctttg ccataacgct
    18361 ggttgccgcc agcaaaaatg ttaaccctac gccccacttg ttcatcgccc gactctctta
    18421 tgttgtgttg tgatgagcaa agcgtgccag aaggttaacc aaacattaaa gaataaaaaa
    18481 agattggcta taacttgcgg gtatatgttg aaggattaaa aaggcggaag aaatccgcct
    18541 catattgctg acaaagtccg ctttgtccat gccggatgcg gcgcgaacgc cttatccggc
    18601 ctacaaaagt ttgcaaattc aataaattgc agaattaatg taggcctgat aagcgaagcg
    18661 catcaggcat ttttgcttct gtcatcggtt tcaggctaaa tgaatctgcc tttttccgaa
    18721 atcattaata cagttttttc gcgcagtcca gccagtcgcc tttgaacgga cgcttcatgt
    18781 tttcgatagc gtcgatgatg tcgtggtgaa ccaactgttc gttctggata cctacgcaac
    18841 gaccgccgta acctgccagc agcagatcga tagcgtaagc gcccatacgg gaagccagaa
    18901 tacggtcgta aggcaccgga gaaccaccgc gctggatgtg gcccagcaca gttgcgcggg
    18961 tttcacgacc ggtttctttc tcgatgaaat gcgccagttc gtcaacatca cacatatgtt
    19021 cggtaatcgc cacgatcgcg tgttttttac ctttcgcgat acccgctttg atttcgttta
    19081 ccaggtcttc acggctgaat tcaacttccg gaaccacaac gaattcacag ccaccggcaa
    19141 tggccgcagc cagggtcaga tcgccacaat aacggcccat cacttccacc acggaaatac
    19201 gctggtgaga agaagaggtg tcacgcagac ggtcgatcgc ttctacaacg gtgctcagcg
    19261 cagtgaagaa accgatagtg tagtcagtgc ctttgatgtc gttgtcgata gtgcccggca
    19321 gaccgatgca cgggaagccc atttcggtca gacgcattgc acccatgtag gaaccgtcac
    19381 cgccgataac caccagcgcg tcgataccac gttttttcag gttttcgata gccacggcgc
    19441 ggatgttctc gtcgcggaat tccgggaaac gcgcagaacc gaggaacgta ccgccacggt
    19501 tgatcatgtc agacacgctg taacggtcta gctgtaccat acggtcttca tacagaccca
    19561 gatagccgtc ataaataccc attacttcca gaccttctgt cagcgcagaa cgaacaaccc
    19621 cgcgaattgc ggcgttcatg cctggcgcat caccgccgct tgtcaacaca ccgattttct
    19681 taatcatgac tacctctgaa ctttggaatg caaaatgaaa tctgttgccg gaagtcttct
    19741 tgcacatcga agtgatccaa cgaatgtgca aatagtataa caatcacttc ctgctgaatt
    19801 gattcaggtc aggccaaatg gcggtatttt atacacaaaa tgcgggtctg gctctctttt
    19861 atactgatta tgaaagcata gaccgtttac cctccctggg tacgacggaa caggggtcct
    19921 gatggataat tacatccgat cccggaaaac gccgtaaaat agcctgctct acctgatccg
    19981 ccaccatatg tgcctgaacc aaaggcagag agtcttccat ttccaaatga atctgaataa
    20041 agcgggtcgg ccctgactgc cgcgtgcgaa gatcgtgagc gccgctaaca cccggccagg
    20101 aagtcacgat atcaataatt tcttgccgtt cctcatcagg caatgcgcga tccagtaatg
    20161 actgtaccgc ctcatatccc atgcgtaacg cgctatataa aatatagatg ccgattccca
    20221 atgcaaacag agcatcggcg cgatgccagc cgtaccagga caaccccagc gccagcagaa
    20281 ttgcgccgtt catcataaca tcagactggt aatgtagcat atcagcccgc accgcctggc
    20341 tttgcgtccg ccgcaccacc caacgctgaa acgagacaag gataatcgta caaattagcg
    20401 ccacaattgt cacgataacc ccgacgcctg gatctgtcat cggtgttgga gatatcagat
    20461 gttgaatacc cgtcaaaaac aggaatagtg ccgaaccgga gataaacata ctttgcgcca
    20521 gcgccgcgag ggactcagct ttaccgtgac caaacgagtg attatcgtcg gcaggttgca
    20581 gggaatatcg caccaccaat aaattcgtca acgacgcgcc gatatccacc agcgaatcca
    20641 ccagcgcggc gagaatactc accgacccgg tataccacca tgcaaaaatt ttaatcagca
    20701 atagcagcga agccatcgcc gtcgcagcaa tcgccgcccg actgaccagc cgtccataag
    20761 attgattcat aaatactccc gctatcaact gacgctagta taacggaagc aaatcatctg
    20821 caatgcatta agcagcaggc aaattgagga taaaaaaaac ccccacatca tgtgggggaa
    20881 gacagggatg gtgtctatgg caaggaaaac agggtttact actgggaacg tgagttgcta
    20941 ctactcaata gcttcaacga tgaacttttt tgccattgcg tcacgtcacg caactgctcc
    21001 attcgttgtt gatgtttctc gtttaaaacc gcttgctgct ccggcgttaa caggcgatac
    21061 atttggttgc ggacttttgc catctcaacc tgacgagcaa tttgctcatt cgccattttt
    21121 tctgcctgtg cgcgcacagc gttttcatca aaattttctg cggtgacaag gcgatgcatt
    21181 gtctccagtt cgctaacatt aacaggaggc tgttcgtgcc gggcctgttg cataagatct
    21241 cgcatctgct gacgctgatg ttcggttaaa cttatgccgt cgaacatatg gctctgcgta
    21301 ctgcgctgcg taagttcttc acccggatgc cagttatcgc ctgaaccgac ttcagcagcg
    21361 tggcttaatg aactgactgc cagcgttgag gccatgacgg cagcggtaac tatgcgcatc
    21421 atttgctccc aaaatctttc tgtcgcgatt caacgataga gagtttacga ttcaggctgc
    21481 aaacatgcgt cagggggtgt aaaacaacgt aaagtcatgg attagcgacg tctgatgacg
    21541 taatttctgc ctcggaggta tttaaacaat gaataaaatc ctgttagttg atgatgaccg
    21601 agagctgact tccctattaa aggagctgct cgagatggaa ggcttcaacg tgattgttgc
    21661 ccacgatggg gaacaggcgc ttgatcttct ggacgacagc attgatttac ttttgcttga
    21721 cgtaatgatg ccgaagaaaa atggtatcga cacattaaaa gcacttcgcc agacacacca
    21781 gacgcctgtc attatgttga cggcgcgcgg cagtgaactt gatcgcgttc tcggccttga
    21841 gctgggcgca gatgactatc tcccgaaacc gtttaatgat cgtgagctgg tggcacgtat
    21901 tcgcgcgatc ctgcgccgtt cgcactggag cgagcaacag caaaacaacg acaacggttc
    21961 accgacactg gaagttgatg ccttagtgct gaatccaggc cgtcaggaag ccagcttcga
    22021 cgggcaaacg ctggagttaa ccggcactga gtttaccctg ctctatttgc tggcacagca
    22081 tctgggtcag gtggtttccc gtgaacattt aagccaggaa gtgctgggca aacgcctgac
    22141 gcctttcgac cgcgctatcg atatgcacat ttccaacctg cgtcgtaaac tgccggatcg
    22201 taaagatggt cacccgtggt ttaaaacctt gcgtggtcgc ggctatctga tggtttctgc
    22261 ttcatgatag gcagcttaac cgcgcgcatc ttcgccatct tctggctgac gctggcgctg
    22321 gtgttgatgt tggttttgat gttacccaag ctcgattcac gccagatgac cgagcttctg
    22381 gatagcgaac agcgtcaggg gctgatgatt gagcagcatg ttgaagcgga actggcgaac
    22441 gatccgccca acgatttaat gtggtggcga cgtctgttcc gggcgattga taagtgggca
    22501 ccgccaggac agcgtttgtt attggtgacc accgaaggcc gcgtgatcgg cgctgaacgc
    22561 agcgaaatgc agatcattcg taactttatt ggtcaggccg ataacgccga tcatccgcag
    22621 aagaaaaagt atggccgcgt ggaactggtc ggtccgttct ccgtgcgtga tggcgaagat
    22681 aattaccaac tttatctgat tcgtccggcc agcagttctc aatccgattt cattaactta
    22741 ctgtttgacc gcccgctatt actgctgatt gtcaccatgt tggtcagtac gccgctgctg
    22801 ttgtggttgg cctggagtct ggcaaaaccg gcgcgtaagc tgaaaaacgc tgccgatgaa
    22861 gttgcccagg gaaacttacg ccagcacccg gaacttgaag cggggccaca ggaattcctt
    22921 gccgctggtg ccagttttaa ccagatggtc accgcgctgg agcgcatgat gacctctcag
    22981 cagcgtctgc tttctgatat ctctcacgag ctgcgcaccc cgctgacgcg tctgcaactg
    23041 ggtacggcgt tactgcgccg tcgtagtggt gaaagcaagg aactggagcg tattgaaacc
    23101 gaagcgcaac gtctggacag catgatcaac gatctgttgg tgatgtcccg taatcagcaa
    23161 aaaaacgcgc tggttagcga aaccatcaaa gccaaccagt tgtggagtga agtgctggat
    23221 aacgcggcgt tcgaagccga gcagatgggc aagtcgttga cggttaactt cccgcctggg
    23281 ccgtggccgc tgtacggcaa tccgaacgcc ctggaaagtg cgctggaaaa tattgttcgt
    23341 aatgctctgc gttattccca tacgaagatt gaagtgggct ttgcggtaga taaagacggt
    23401 atcaccatta cggtggacga cgatggtcct ggcgttagcc cggaagatcg cgaacagatt
    23461 ttccgtccgt tctatcggac cgatgaagca cgcgatcgtg aatctggcgg tacaggattg
    23521 gggctggcga ttgttgaaac cgccattcag cagcatcgtg gctgggtgaa ggcagaagac
    23581 agcccgctgg gcggtttacg gctggtgatt tggttgccgc tgtataagcg gagttaaact
    23641 ccgcatttgt aggcaggata aggcgtttac gccgcatccg gcatttgagc aggatgcctg
    23701 atgcgacgct gatagcgtct tatcaggcct acactccggg ggttattttc cccacaaccg
    23761 gcgggaaaaa tcttcgatct tgccgctcag tcggcgcttt tgcatcgtcc gcgtccagct
    23821 tttcgataac ccggcggcgg agagtaaacg gtgatattga tcgtcatcaa acggcatatg
    23881 ccatgcgatg gcggcagcct cctgcacggt cacatcgctg acacgggaaa ccaactccag
    23941 cggcgcgtcc gcagacactt tccctggcgc tatcacgcta tacaaccacc ccaccttacc
    24001 tgtgttttgc atcaactgcg ctatatcgct gatatcaaaa tggtaattga gtttatagca
    24061 aggcgagcgc ggctggctga cctgaattaa tgcctctccc cagcggaaaa tatcgcccat
    24121 ataaacatta ctttccgtca ggccgtcggt tgagaggttt tcaccaaacg caggcgcaac
    24181 aaacaactcc gcctgttccg gaaattcccg cgcccagtag agataatgct cacgaggata
    24241 atgacacagc gctctgtctg gcccgccgtg aacctttttc tccgcctgct cgtcaccttc
    24301 cagccccagc tccgtcagca tcaactcacc atcaacctgg attttggcaa ttgcgctggg
    24361 tttgccttcg ggataagcct gaatcttgcc tgtgtataca tcaaccggat atcgcatctg
    24421 ttccctctcc acgagaaatt tccccattag accatattta ttttatgtca ggcgtgctgt
    24481 tttcgcggta agtatccagg caggaactca atagcaaacc ggacgaacgt tagttcatcc
    24541 ggctgtgggg attaaggaag caggtttatt tcaccgtacc taaaatttcg attttcgctg
    24601 ctctggcttt gactttacgt gaccagatag aggtaagaat tggcaccaga atcgaagtca
    24661 caatgaccgc cgtcgctacc agcgaagttg ctgccggagc catcggttta aacgcaggca
    24721 ccatttctgc aatcagcaca ggtgtcgcta ccgcggcccc tgcggaactg gaagcggcaa
    24781 ttccggcagt gccatcgcca ccgccaatca atttatcggc gataatcaac ggaataccgg
    24841 tcacgataat tactgccaca cccaacagga tccccagcaa accagtctgg gcaatcacag
    24901 tcaaatcaat ggtattgccc agcgcgaagg caaagaatgg aatcagcgtt tgcaccgctt
    24961 tgctgaaaaa ttctcgtaat tcagggtcaa ggttccccag ggcaaagccc accaggaacg
    25021 gtaatactgc gccgacgaaa acatgcggtt caaacgaggc aatccctgca gtgcccagaa
    25081 taatcatcgt catgagcggc ccggactcca acgacatcaa cacaaatgcc ccagcttctt
    25141 cttttgtgcc gtactgctgc atgatggaag cgtaaagtcc gccattggtc atatccatcg
    25201 ccgccaccag cgccagcgtt gaaagtccgg caaagaatcc aacttcaaca ccatgttccg
    25261 gaataatgcg cgaggcaatt gccgcaacca cccacgcgac ggcaattttt gttaccacca
    25321 gcgtaccgga tttacgcaat accgttcccg tcgcgcttaa ttttattgac gcccccatgc
    25381 aaaaaaacca caccgccaga atgggcaccg taccggtaat catcccgttg gtgaatgatc
    25441 caaaatattt ccccgccccc ggcgagaagg tgtggcacag tgcgccaagg aatagcggga
    25501 cgagcatcat cccccccgag attttctcaa tcgagcgttt tatctgcatt tccatcacct
    25561 gtcggcatat ttttaagggc tgatgcgtca ttcctcaatg gagcatcagt gttgatggaa
    25621 acgaagatag acattcattt aatcaataaa agtgatcaaa accacatcaa ttgaaacact
    25681 attttatttt tatcggatca ttgtttttat tttgaaattg atcacaaaaa aaccaccgcc
    25741 gttggcgatg gttcattttt taagctgata tgcggcctat acgcctcatt gcagcaggcg
    25801 gcaaatgatt attttttcgc cgcgaaacgc gccgcagctt cgtcccagtt caccacgttc
    25861 cagaactctt tgatgtagtc cgggcggcgg ttctggaatt tcaggtagta agcatgttcc
    25921 cacacatcca ggcccagaat cgggaagccg gaagcgccag aaatagcttc acccatcagc
    25981 ggagaatcct ggttagcagt agaaaccacc gccagtttat cgcctttcag caccagccat
    26041 gcccagccgg aaccaaagcg ggaagctgcc gctttttcaa attctgcttt gaagttatca
    26101 acggagccga agtcacgttc gatagccgct ttcaggtcac cctgcagggt ggtgcctttt
    26161 ttcagacctt tccagaacag gctgtggtta gcgtgaccgc cagcgttgtt gcgcagtacg
    26221 gttttcttgt ctgctggcag ctggtccagt ttggtgatca gctcttcaac cggcaggttg
    26281 gcaaattctg gcaggctttc cagcgccgcg ttggcgttgt ttacgtaggt ctgatggtgt
    26341 ttggtgtggt ggatttccat ggtctgctta tcgaagtgcg gttccagggc atcgtaagca
    26401 tacggcaggg atggcagggt atagctcata ttcatctcca gtattgtcgg gcggccgatt
    26461 gttaatgccg cgtaagcagt tggttcatta tagttaatta aatgatattg aaaatgatta
    26521 tcaatgccgt acttttcgta agggtatggt tttgcaggaa aatgcccgag atgtgaagca
    26581 aatcacccac ttaatgccgt gattgccagt aaatcgacaa cggcggcaac aggcgaaagg
    26641 ttaatcgaca gcacgatttt tacactcatc tcgtcggaga tgtgaagcga cgaaaaatga
    26701 tgaggataag aagatgagta acgcgattac gatggggata ttttggcatt tgatcggcgc
    26761 ggccagtgca gcctgttttt acgctccgtt caaaaaagta aaaaaatggt catgggaaac
    26821 catgtggtca gtcggtggga ttgtttcgtg gattattctg ccgtgggcca tcagcgccct
    26881 gttactaccg gatttctggg cgtattacag ctcgtttagt ctctctacgc tgctgcctgt
    26941 ttttctgttc ggcgctatgt gggggatcgg taatatcaac tatggcctga ccatgcgtta
    27001 tctcggcctg tcgatgggaa ttggcatcgc cattggcatt acgttgattg tcggtacgct
    27061 gatgacgcca attatcaacg gcaatttcga tgtgctgatt aacaccgaag gcggacgcat
    27121 gacgttgctc ggcgttctgg tggcgctgat tggcgtaggg attgtgactc gcgccgggca
    27181 gttgaaagag cgcaaaatgg gcattaaagc cgaagagttc aacctgaaaa aagggctggt
    27241 gctggcggtg atgtgcggca ttttctctgc cggtatgtct tttgcgatga acgccgcaaa
    27301 accgatgcat gaagccgctg ccgcactggg cgtcgatcca ctgtatgtcg ctctgccaag
    27361 ctatgtcatc atcatgggcg gcggcgcgat catcaacctc ggtttctgct tcattcgtct
    27421 ggcaaaagtg aaggatttgt cgctaaaagc cgacttctcg ctggcaaaac cgctgatcat
    27481 tcacaatgtg ttactctcag cactgggcgg gttgatgtgg tatctgcaat tctttttcta
    27541 tgcctagggc cacgcccgca ttccggcgca gtatgactac atcagttgga tgctgcatat
    27601 gagtttctat gtattgtgcg gcggtatcgt cgggctggtg ctgaaagagt ggaacaatgc
    27661 aggtcgccgt ccggtaacgg tgttgagcct cggttgtgtg gtgattattg tcgccgccaa
    27721 catcgtcggc atcggcatgg cgaattaatc tttctgcgaa ttgagatgac gccactggct
    27781 gggcgtcatc ccggtttccc gggtaaacac caccgaaaaa tagttactat cttcaaagcc
    27841 acattcggtc gaaatatcac tgattaacag gcggctatgc tggagaagat attgcgcatg
    27901 acacactctg acctgccgca gatattgatt gatggtcatt ccagtctgct ggcgaaattg
    27961 ctgacgcaaa acgcgctcac tgcacgatgc ctcatcacaa aatttatcca gcgcaaaggg
    28021 acttttcagg ctagccgcca gccgggtaat cagcttatcc agcaacgttt cgctggatgt
    28081 tggcggcaac gaatcactgg tgtaacgatg gcgattcagc aacatcacca actgcccgaa
    28141 cagcaactca gccatttcgt tagcaaacga cacatgctga ctactttcat gctcaagctg
    28201 accgataacc tgccgcgcct gcgccatccc cacgctacct aagcgccagt gtggttgccc
    28261 tgcgctggcg ctaaatcccg gaatcgctcc ctgccagtca agattcagct tcagccgctc
    28321 cgggcaataa ataatattct gcaaaaccag atcgttaacg gaagcgtagg agtgtttatc
    28381 gtcagcatga atgtaaaaga gatcgccacg ggtaatgcga taagggcgat cgttgagtac
    28441 atgcagacca ttaccgcgcc agacaatcac cagctcacaa aaatcatgtg tatgttcggc
    28501 aaaaacatct tgcggataac ggtcagccac agcgactgcc tgctggtcgc tggcaaaaaa
    28561 atcatctttg agaagtttta actgatgcgc caccgtggct acctcggcca gagaacgaag
    28621 ttgattattc gcaatatggc gtacaaatac gttgagaaga ttcgcgttat tgcagaaaac
    28681 catcccgtcc ctgacgaata tcacgcggtg accagttaaa ctctcggcga aaaagcgtcg
    28741 aaaagtggtt actgtcgctg aatccacagc gataggcgat atcagtaacg ctggcctcgc
    28801 tgtggcgtag tagatgtcgg gctttcatca gtcgcaggcg gttcaggtaa cgctgaggcg
    28861 tcagtcccgt ttgctgctta agctgccgat gtagcgtacg cagcgaaaga gaaaattgct
    28921 ccgccacggc atcccaattc acctcatcgg caaaatggtc ctccagccag accagaagca
    28981 agttgagacg tgatgcgctg ttttccaggt tctcctgcaa actgctttta cgcagcaaga
    29041 gcagtaattg cataaacaag atctcgcgac tggcggtcga gggtaaatca ttttcccctt
    29101 cctgctgttc catctgtgca accagctgtc gcacctgctg caatacgctg tggttaacgc
    29161 gccagtggga aggatactgc ccatccagct cttgtggcag caactgattc agcccggcga
    29221 gaaactgaaa tcgatccggc gagcgataca gcacattagt cagacacaga ttatcggtat
    29281 gttcatacag atgccgatca tggtcgcgca caaaacagac cgtgccaccg gtgatggtat
    29341 agggctgccc attaaacaca tgaatacccg tgccatgttc gacaatcaca atttcatgaa
    29401 aatcatgatg atgttcagga aaatccgcct gcgggagccg gggttctatc gccacggacg
    29461 cgttaccaga cggaaaaaaa tccacactat gtaatacggt catactggcc tcctgatgtc
    29521 gtcaacacgg cgaaatagta atcacgaggt caggttctta ccttaaattt tcgacggaaa
    29581 accacgtaaa aaacgtcgat ttttcaagat acagcgtgaa ttttcaggaa atgcggtgag
    29641 catcacatca ccacaattca gcaaattgtg aacatcatca cgttcatctt tccctggttg
    29701 ccaatggccc attttcctgt cagtaacgag aaggtcgcga attcaggcgc tttttagact
    29761 ggtcgtaatg aaattcagca ggatcacatt atgacctttc gcaattgtgt cgccgtcgat
    29821 ctcggcgcat ccagtgggcg cgtgatgctg gcgcgttacg agcgtgaatg ccgcagcctg
    29881 acgctgcgcg aaatccatcg ttttaacaat gggctgcata gtcagaacgg ctatgtcacc
    29941 tgggatgtgg atagcctgga aagtgccatt cgccttggat taaacatggt gtgtgaggaa
    30001 gggattcgta tcgatagcat tgggattgat acctggggcg tggactttgt gctgctcgac
    30061 caacagggtc agcgtgtggg cctgcccgtt gcttatcgcg atagccgcac caatggccta
    30121 atggcgcagg cacagcaaca actcggcaaa cgcgatattt atcaacgtag cggcatccag
    30181 tttctgccct tcaatacgct ttatcagttg cgtgcgctga cggagcaaca acctgaactt
    30241 attccacaca ttgctcacgc tctgctgatg ccggattact tcagttatcg cctgaccggc
    30301 aagatgaact gggagtacac caatgccacc accacacaac tggtcaatat caatagcgac
    30361 gactgggacg agtcgctact ggcgtggagc ggggccaaca aagcctggtt tggtcgcccg
    30421 acgcatccgg gtaatgtcat tgggcactgg atttgcccgc agggtaatga gattccggtg
    30481 gtcgccgttg ccagccatga taccgccagc gcgcttatcg cctcgccgtt aaacggttca
    30541 cgcgccgctt atctctcttc tggcacctgg tcattgatgg gcttcgaaag ccagacgcca
    30601 tttaccaatg acacggcgct ggcagccaac atcaccaatg aaggcggggc ggaaggtcgc
    30661 tatcgggtgc tgaaaaatat tatgggctta tggctgcttc agcgagtgct tcaggagcgg
    30721 caaatcaacg atctcccggc gcttatcgcc gcgacacagg cacttccggc ctgccgcttc
    30781 atcatcaatc ccaatgacga tcgctttatt aatcctgacg agatgtgcag cgaaattcag
    30841 gctgcgtgtc gggaaacggc gcaaccgatc ccgggaagtg atgctgaact ggcgcgctgt
    30901 attttcgaca gtctggcgct gctgtatgcc gatgtgttgc atgagctggc gcagctgcgt
    30961 ggtgaagatt tctcgcaact gcatattgtc ggcggtggct gccagaacgc gctgctcaac
    31021 cagttatgtg ccgatgcctg cggtattcgg gtgatcgccg ggccagttga agcctcgacg
    31081 ctcggcaata tcggcatcca gttaatgacg ctggatgaac tcaacaatgt ggatgatttc
    31141 cgtcaggtcg tcagcaccac cgcgaatctg accaccttta tccctaatcc tgacagtgaa
    31201 attgcccact atttggcgca gattcactct acacgacaga caaaggagct ttgcgcatga
    31261 ccactcaact ggaacaggcc tgggagctgg cgaaacagcg tttcgcggcg gtggggattg
    31321 atgtcgagga ggcgctgcgc caacttgatc gtttacccgt ttcaatgcac tgctggcagg
    31381 gcgatgatgt ttccggtttt gaaaacccgg aaggttcgct gaccggtggg attcaagcta
    31441 ctggtaatta tccgggcaaa gcgcgtaatg ccagtgagct acgtaccgat ctggaacagg
    31501 ctatgcggct gattccgggg ccgaaacggc ttaatttaca tgccatctat ctggaatcag
    31561 acacgccagt ctcgcgcgac cagatcaaac ctgagcactt caaaaactgg gttgaatggg
    31621 cgaaagccaa tcagctcggt ctggatttta acccctcctg tttttcgcat ccgctaagcg
    31681 ccgatggctt tacgctttcc catgccgacg acagcattcg ccagttctgg attgatcact
    31741 gcaaagccag ccgtcgcgtt tcggcctatt ttggcgagca actcggcaca ccatcggtga
    31801 tgaacatctg gatcccggat ggtatgaaag atatcaccgt tgaccgtctc gccccgcgtc
    31861 agcgtctgct ggcagcactg gatgaggtga tcagcgagaa gctaaaccct gcgcaccata
    31921 tcgacgccgt tgagagcaaa ttgtttggca ttggcgcgga gagctacacg gttggctcca
    31981 atgagtttta cctggggtat gccaccagcc gccagacggc gctgtgcctg gacgccgggc
    32041 acttccaccc gactgaagtg atttccgaca agatttccgc cgccatgctg tatgtgccgc
    32101 agttgctgct gcacgtcagt cgtccggttc gctgggacag cgatcacgta gtgctgctgg
    32161 atgatgaaac ccaggcaatt gccagtgaga ttgtgcgtca cgatctgttt gaccgggtgc
    32221 atatcggcct tgacttcttc gatgcctcta tcaaccgcat tgccgcgtgg gtcattggta
    32281 cacgcaatat gaaaaaagcc ctgctgcgtg cgttgctgga acctaccgcc gagctgcgca
    32341 agttggaagc ggcgggcgat tacactgcgc gtctggcact gctggaagag cagaaatcgt
    32401 tgccgtggca ggcggtctgg gaaatgtatt gccaacgtca cgatacccca acgggtagcg
    32461 aatggctgga gagcgtgcgg gcttatgaga aagcgatttt gagccagcgt gggtaaacac
    32521 tgccggatgc ggcacgagcg ccttatccgg cctacgggtc gaatgtccat gcgggctgtc
    32581 accctcaccc cggccctctc cctgaaaggg cgagggagaa aaacatagcc tcgtgcggca
    32641 acggagctga acactgctcc ggacagtccc ctcgccccta cggggagagg gttagggtga
    32701 agggaacaag cagcaccatg tctaaacaag gcaacctaat ttacaggaac acagaacatg
    32761 caaaacatta cccagtcctg gtttgtccag ggaatgatca aagccaccac cgatgcctgg
    32821 ctgaaaggct gggacgagcg caacggcggt aacctgacgc tgcgcctgga tgacgccgat
    32881 atcgcgcctt ataaagacaa cttccatgca caaccgcgtt atatcccgct cagccaaccc
    32941 atgcctttac tggcaaatac accgtttatt gtcaccggct ccggcaaatt cttccgtaac
    33001 gtccagcttg atcctgcagc taacttaggc gtcgtaaaag tcgacagcga cggcgcgggc
    33061 taccatattc tctgggggtt aaccaacgaa gccgttccaa cttctgaact tccggctcac
    33121 tttctctccc actgcgagcg cattaaagcc accaacggta aagatcgggt gatcatgcac
    33181 tgccacgcca ccaacctgat cgccctcacc tatgtactgg aaaacgacac cgcggtcttt
    33241 actcgccaac tgtgggaagg cagcaccgag tgtctggtgg tattcccgga tggcgttggc
    33301 attctgccgt ggatggtgcc cggcacggac gaaatcggcc aggcgaccgc acaagagatg
    33361 caaaaacatt cgctggtgtt gtggcccttc cacggcgtct tcggcagcgg gtcgacgttg
    33421 gatgaaacat tcggtttaat cgacaccgca gaaaaatcag cacaagtatt agtgaaggtt
    33481 tattcgatgg gcggcatgaa acagaccatc agccgtgaag agttgatagc gctcggcaag
    33541 cgcttcggcg ttacaccact agccagtgcg ctggcgctgt aaggagcaaa catgatccgc
    33601 aaagcctttg tcatgcaggt taaccccgac gctcacgaag agtatcagcg tcggcataac
    33661 cccatctggc cggaactgga agcagtgttg aaatctcacg gtgcgcataa ctacgccatt
    33721 tacctcgaca aagcgcgtaa tctgctgttt gccacggtag agattgaatc tgaagagcgc
    33781 tggaatgcgg ttgccagtac tgatgtttgc caacgttggt ggaaatatat gaccgacgtc
    33841 atgcccgcga acccggataa cagcccggtg agtagcgaac tgcaagaagt gttttacctg
    33901 ccgtaattgc ctaatgtgcc ggagacgcct ccggcacaat atttttaatt tcgatttgaa
    33961 aaataaacat attacacctt cagttaatat atcccggtaa cttatatttc cccgatgcat
    34021 tgtgagtaaa tcacaaaaat aatgaataac tcattaatga ttcatgtggt ttatttaaat
    34081 aaccattatg tgcattactc cgcaaatctg acctttcact ctgtctcaaa ttgtcattgt
    34141 ttaccgacat atcgcctctc atcacttaat cgctccacca ttacaattga ttaaaatata
    34201 tttcatggag tccgattaat ggcagctctt actgcaagct gtattgacct gaatattcag
    34261 ggcaatggcg cttattccat tctgaagcag ttggcgacaa tggcgttaca aaacggtttt
    34321 atcaccgact cacaccagtt cctgcaaacc ctgctgctgc gcgaaaaaat gcactctact
    34381 ggatttggtt ccggcatcgc cgtgccgcac ggtaaaagcg cctgcgttaa acaaccgttc
    34441 gtgttattcg cccgcaaagc gcaggctatt gactggaaag ccagcgatgg cgaagacgtc
    34501 aattgctgga tctgcctcgg cgtgccgcaa agcggcgaag aggatcaggt caaaatcatc
    34561 ggcacactgt gtcgcaaaat tattcacaag gaatttattc atcaactgca acagggcgat
    34621 accgaccagg tgcttgcctt gttaaatcaa accctcagct cataaggaag tggcgatgga
    34681 gtcatcctta cgtattgtcg cgatcaccaa ctgccccgcc gggatcgctc acacctacat
    34741 ggtggcggaa gccctggaac agaaagcccg ttctctcggt cataccatta aagttgaaac
    34801 tcaaggatca agcggcgtcg aaaaccgctt atccagcgaa gagattgccg ctgccgatta
    34861 cgtcattctc gctaccgggc gtggcctgag cggtgacgat cgcgcgcggt ttgccgggaa
    34921 gaaggtttat gagattgcca tctcccaggc gctgaaaaat atcgatcaga ttttcagtga
    34981 attaccaaca aactcgcatc tttttgctgc agatagcggc gtgaagcttg gcaaacagga
    35041 agtgcaaagc ggcagcgtga tgagccacct gatggcgggc gtctctgcgg cgctgccgtt
    35101 tgtcatcggc ggtggtatcc tggtggcgct tgccaacatg ctggtgcagt tcggtttgcc
    35161 gtataccgat atgtcgaaag gcgcgccgtc gtttacctgg gtagttgaat ccatcggtta
    35221 ccttggcttc acctttatga tccccatcat gggcgcttat attgcctcgt cgattgccga
    35281 taaaccggct ttcgctccgg cgtttctggt ttgctatctg gcgaacgaca aagcgctgct
    35341 cggcacccag tcgggtgcgg gattcctcgg cgcggtggtg ctggggctgg cgattggcta
    35401 tttcgtcttc tggttccgca aagtgcgcct tggcaaagca ttacaaccgc tgctcggttc
    35461 catgctgatc ccgttcgtta ctctgctggt tttcggcgtg ctcacttact acgttatcgg
    35521 gccggtgatg tccgacctca tgggcgggct gctccacttc ctgaatacca ttcctccatc
    35581 aatgaagttt gcggcagcgt ttctggtcgg tgcgatgctg gcgttcgata tgggcggccc
    35641 catcaataaa accgcctggt tcttctgctt ctcactgctg gaaaaacaca tttatgactg
    35701 gtacgccatc gtcggcgttg tcgcgttgat gccgcccgtt gcggcgggtc ttgcgacgtt
    35761 cattgcgccc aaactgttta cccgacagga aaaagaggct gccagtagtg ccattgtggt
    35821 cggtgccaca gtcgcaaccg aaccggctat accttacgtt ctggcagctc cgttgccgat
    35881 gattaccgcc aatacgctgg cgggtggcat taccggcgtg ctggtgatcg ctttcggaat
    35941 caaacgtctg gcacctggtt tgggtatctt tgacccgctg atcggcctga tgtcgccggt
    36001 gggttcgttt tatctggtgc tggcgattgg cctcgcgctg aacatttcat tcattatcgt
    36061 cctgaaagga ttgtggttac gccgcaaagc gaaagccgcg caacaggagc ttgtccatga
    36121 acattgagtt actgcaacag ttgtgcgaag ccagcgccgt cagcggcgat gaacaggaag
    36181 ttcgcgacat tctgataaac acgctggaac cttgcgttaa tgagatcacc tttgatggtc
    36241 tgggcagctt tgttgcccgt aaggggaata aaggtccaaa agttgccgtt gtcgggcata
    36301 tggatgaagt cggctttatg gtcacccaca tcgacgagag cggttttctg cgtttcacca
    36361 ccattggtgg ctggtggaat cagtcaatgc tcaaccaccg ggtaaccata cgcacacaca
    36421 agggatttaa aatccctggt gtgattggtt ccgtcgcacc tcatgcgtta acggaaaagc
    36481 aaaagcaaca gccgctgtca tttgatgaga tgttcattga tattggcgcg aacagtcgcg
    36541 atgaggtgga aaaacgcggt gtggcgatgg gcgattttat tagcccggaa gccaattttg
    36601 cctgctgggg cgaagataaa gtggtcggca aggcgctgga taaccgcatc ggctgcgcaa
    36661 tgatggctga actattgcag acggtgaata atcccgaaat tacgctgtat ggcgtcggca
    36721 gcgtggaaga agaagttggg ctacgcgggg cacaaacctc ggcggaacac attaaaccgg
    36781 atgtggtgat tgtgctggat accgctgtcg caggtgatgt tccgggcatt gataacatta
    36841 aatacccgct gaaactgggc caggggccgg ggctgatgct gtttgacaag cgctacttcc
    36901 ccaaccagaa actggtagca gcgtttaaaa actgtgccac gcagaatgat ttaccgctgc
    36961 aattttccac catgaaaacc ggagcgacgg atggcgggcg ctacaacgtg atgggcggcg
    37021 ggcgtccggt tgtcgcgctg tgtctgccaa ctcgttatct gcacgccaac agcggtatga
    37081 tttcaaaagc cgattacgat gctctgctca cgctgatacg gggttttctg accaccttaa
    37141 ctgcggagaa agtcaacgcg tttagccagt tccgtcaggt ggattaatgc taaacgaacg
    37201 ccagttaaag attgtcgatc tgctggagca acagccgcgc acgcctggtg agctggcgca
    37261 acagactggc gtttcaggca ggaccatcct gcgtgatatt gactatctca actttaccct
    37321 taacggcaaa gcccgcattt ccgccagtgg tagtgcgggc tatcagctgg aaatcttcga
    37381 gcgccgcagc ttttttcagt tgctgcaaaa gcacgataac gacgatcggc tgctggcact
    37441 gttattactg aataccttta ctccccgtgc gcaactcgcc tcggcgctta atttgccaga
    37501 aacgtgggtc gcagagcgtc tgccccggtt aaaacagcgt tatgaacgcg cttgttgcct
    37561 ggccagccgc cccggtttgg gccatttcat tgatgagaca gaagagaagc gcgttatctt
    37621 gctggcgaac ttgctgcgca aagatccgtt tttaattccg ctggcgggcg taacacgaga
    37681 caaccttcag catttatcca cggcctgcga caaccaacac cgctggccgc tcatgcaggg
    37741 tgattatctc tccagcctga ttctggcgat ttacgccctg cgtaatcaac tgaccgatga
    37801 gtggccgcaa tatcccggtg acgagataaa acaaatcgtt gaacatagcg gtctgtttct
    37861 tggtgataac gctgtaagaa ccctgacagg tttgatagag aaacagcatc agcaagcgca
    37921 gataatttct gccgataatg tgcagcggtt gctgcaaagg gtgccgggca tcgcgtcatt
    37981 gaatattatt gatacgcagc tggttgagaa cattaccggg catttattac gttgccttgc
    38041 cgcaccggtg tggattgctg agcaccgcca aagcagcatg aataacctga aagccgcctg
    38101 gcctgcggcc tttgatatga gtctgcactt tattacgcta ctgcgtgaac agctcgatat
    38161 tccccttttc gacagcgatc tgatcggttt gtattttgcc tgtgcgctgg aacggcatca
    38221 aaacgaacgc cagccgatca ttttgctctc ggaccagaac gcgattgcca ctattaatca
    38281 gctcgccatt gagcgcgatg ttttacattg tcgggtgatt attgcccgta gcttaagcga
    38341 acttgttgcc attcgcgaag agattgagcc gttattgatc attaacaaca gccattattt
    38401 actggatgac gcggtaaata attacatcac cgtaaaaaat atcattacgg ctgccggtat
    38461 cgaacaaata aaacattttc tggcgacggc atttattcgc cagcaac
//
LOCUS       DAZFQD010000031        38404 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.031, whole genome shotgun sequence.
ACCESSION   DAZFQD010000031 DAZFQD010000000
VERSION     DAZFQD010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 38404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38404
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.031"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..219
                     /locus_tag="ACHKTW_001677"
     CDS             <1..219
                     /locus_tag="ACHKTW_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6462724.1"
                     /translation="SICSLFIVNSNMASISLGNESFSTDDDLEYGYLINAGNHYDVYL
                     PPELFAQAYKLKNKEMNAQLDYLNRYAI"
     gene            513..1754
                     /gene="agp"
                     /locus_tag="ACHKTW_001678"
     CDS             513..1754
                     /gene="agp"
                     /locus_tag="ACHKTW_001678"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HFO6462725.1"
                     /translation="MNKTLIAATVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAQQGMVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPVITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDKQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRTSAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDSVLNEAVK"
     gene            complement(1792..2019)
                     /gene="yccJ"
                     /locus_tag="ACHKTW_001679"
     CDS             complement(1792..2019)
                     /gene="yccJ"
                     /locus_tag="ACHKTW_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HFO6462726.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKTDKDSLFWGEQTIERKNV"
     gene            complement(2040..2645)
                     /gene="wrbA"
                     /locus_tag="ACHKTW_001680"
     CDS             complement(2040..2645)
                     /gene="wrbA"
                     /locus_tag="ACHKTW_001680"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HFO6462727.1"
                     /translation="MSVKARILVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPE
                     TMPPQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWA
                     SGALYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVTQVRGG
                     TPYGATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            2697..4340
                     /locus_tag="ACHKTW_001681"
     CDS             2697..4340
                     /locus_tag="ACHKTW_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFO6462728.1"
                     /translation="MKLLATYIRWSTKEQDSGDSLRRQTNLIDAFYSKHKNDYYLLPA
                     HRYVDKGKSGFHQQHKAQGSDFRRMFENVMSGVIPEGSLIVVENFDRFSRADIDTAID
                     DVRQILRKGVSILTLGDGELYDKSALTDPVKLIKHIIIAERAHQESLVKQKRIAQVWN
                     HKTQLARELKKPMGKQAPGWLELSEDGSHYIVDEDKASLVNIIYDKRLSGMSMFAICK
                     WLNEQGYPTINQRKVRISKTKKPDGNWSALSVKHILTSRSVLGYLPAKISTEDRKTVL
                     REEIEGFYPQIVTDSKFYAVQQLLEETGKGKTSSGDHWLYVNILKGLIRCKCGLVMTP
                     TGIRKPVYQGTYRCNGNKESRCSYGTVSRKLLDTQLCSRLFSKLSQLHDEATDTAKLD
                     ELQRRLNTVDSELEKLTETLIQLPNITQIQEALRVKQEEKDELIVQLSREKARVKSVS
                     SLDLSGLDMESVEGRTEAQIIIKRLVKEIVVSGNEKLVDIYLHNGNMIRGFPLDGKDD
                     HTLTLEEATDEMQPLDDMLIFGEPVTRIYPAGDMEEVDA"
     gene            complement(4360..5133)
                     /locus_tag="ACHKTW_001682"
     CDS             complement(4360..5133)
                     /locus_tag="ACHKTW_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000640586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate protein"
                     /protein_id="HFO6462729.1"
                     /translation="MIFSNQIRYSGKDGVYFHLRDNVDAFLTLSATENMEFDSPMQVT
                     TQNMQSGQTVTDNVQRAPRTITISGVVVVGYEGSLLLTRQGQLVENFIDTLESWRDQK
                     QIISVICKDGIKIDDSIITSFKASKDVGISNGLRVQLTFQEINFKAVVGQTDISAATG
                     KTATTNDGGATSKKNAGNVTTERNNAPMLMCQELYSYSASELSDEALKARSNCSKNVT
                     VKDGVSTFTASANEQASKVLKSGNALRKYSVNPNKKGTY"
     gene            complement(5297..7429)
                     /locus_tag="ACHKTW_001683"
     CDS             complement(5297..7429)
                     /locus_tag="ACHKTW_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001689732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFO6462730.1"
                     /translation="MSKFTVDSFIVELGFNENVIKGLQRVEKAALQSAQRIEKNLNKG
                     FRINTKQLDSNLTASLGQLERKFNKAFDKIEQRARNTKAFQFTTRFNDTVNPPKQPRQ
                     PRISGNRAITAAYSANMSKLKGLNPIVEKYIKSQFYGLSAKAGSMDSSKFNEKLAQLN
                     ASVREAIAKARGHTSTSINGDRAANSLDNLSSAAVKTAGAFISMQAVLASYQKIMEIG
                     LQRASAERSIDFVFDKDAGQVKEFTKSLAQTTGLDIAELQSQFAGFGASAKESMGIQG
                     SEELFKNMIGYARLMGRSEEEIKRALTALSQMAGKGQIMAEELKGQLAEAVPGMVQVF
                     AKATGKSEQELFDAMKKGALKSSDTLQKVTQELNKQITAKGGWKAISESTQAQLGNLK
                     NSWNTTLDSIFRGSENGLQDFTRSLTTLLNALGGSGKTLGESFGSLMSDMSHGVDNLT
                     DISYRVRAFFDEVTLAYRSLNDTQKKIVDGLSDGLIDGLKMLAVAVTAQKAANVAGGL
                     LNLANAVTSFGDAVNKAKAGKAGSSGGALSKVGRVVLYAELVNFALDNLNPEAAKNPN
                     LHQSGIGTETNFNPEYKGDSSKPNQINEGWAKIGSSIADWFNGAANKIANFQEPMSNL
                     VNNTQSTPPAITGDMVNSNAQLAMAVSELGRKVDALTAEAKKPLSVNITGEATVRPDE
                     PAFFTFNQSANDQFTQDMLLSSSYPEEE"
     gene            complement(7546..7758)
                     /locus_tag="ACHKTW_001684"
     CDS             complement(7546..7758)
                     /locus_tag="ACHKTW_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6462731.1"
                     /translation="MFEERLETIRSVCERLQLQPNRQKLRIKNNRHYITSHKPKTRKI
                     PKWCIDRIPSDAQIIGETELHYLVRH"
     gene            complement(7782..8486)
                     /locus_tag="ACHKTW_001685"
     CDS             complement(7782..8486)
                     /locus_tag="ACHKTW_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001461099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6462732.1"
                     /translation="MSIRNEHEMIVENRPSALDIHELSLRESFDRAWRESHEVNKGPY
                     IPPEPLEIPRVEIDFSMNERCELDLKLKLQRRYFQAIKDSQEAMRSVYEKIQSKLEDE
                     IDEMAILMELQTNSYAYFSRLDRDGWGYDPVEIGKVIADLPEGQRVIRTERAVRAPHR
                     LSLIVADKSQAELEEIAKEKLMKARENEINLLKSRLAKLISDHQALAKEYRQELAQII
                     SFNDFLSVPREFSKAV"
     gene            complement(9059..9382)
                     /locus_tag="ACHKTW_001686"
     CDS             complement(9059..9382)
                     /locus_tag="ACHKTW_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6462733.1"
                     /translation="MSNYELQIFVDNDTAMMVQSFIDAGVEIDFDRLLKLMAENSEAI
                     EDFIQGIETGEPRFMFPVTDSNMKRLIVEETNRYSVSPEKYLKAAIAILYADNILVTD
                     SRVVH"
     gene            complement(9757..9987)
                     /locus_tag="ACHKTW_001687"
     CDS             complement(9757..9987)
                     /locus_tag="ACHKTW_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6462734.1"
                     /translation="MLPRYKFYKLASHLIRQDSRSKEYEDVTLDRNKKRLKPHQIEQL
                     RYELKSLDIELDYSDPTQFKRVYELIKNWGKE"
     gene            complement(9987..10274)
                     /locus_tag="ACHKTW_001688"
     CDS             complement(9987..10274)
                     /locus_tag="ACHKTW_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6462735.1"
                     /translation="MKNRHTLTRITKTAFEELNRIKNITGLPHSAVMKLAINRDINEQ
                     DLLNSRLEAARHHIRIKQDDLDSLKSLNAFGLDLARLLSIKIHKLADKLEI"
     gene            complement(10276..10831)
                     /locus_tag="ACHKTW_001689"
                     /pseudo
     CDS             complement(10276..10831)
                     /locus_tag="ACHKTW_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999794.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            11179..11367
                     /locus_tag="ACHKTW_001690"
     CDS             11179..11367
                     /locus_tag="ACHKTW_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000247758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6462736.1"
                     /translation="MVNLPPELGSPDVWTGGGVGLIAGLLLRYFIVRVFVVFKLFGRE
                     WSFDLKDRTRTDNHTEPR"
     gene            14182..14355
                     /gene="ymdF"
                     /locus_tag="ACHKTW_001691"
     CDS             14182..14355
                     /gene="ymdF"
                     /locus_tag="ACHKTW_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HFO6462737.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGQHSGGNFKNDPQRASEAG
                     KKGGKSSHGKSDN"
     gene            complement(14438..15766)
                     /gene="rutG"
                     /locus_tag="ACHKTW_001692"
     CDS             complement(14438..15766)
                     /gene="rutG"
                     /locus_tag="ACHKTW_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015218 - pyrimidine nucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006864 - pyrimidine nucleotide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HFO6462738.1"
                     /translation="MAMFGFPHWQLKSTSTESGVVAPYERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNLLG
                     LGKAVDFTLVSHAAWFGLPHFSTPAFNSQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNRVDLSQNGNLIMVAVTL
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRKLVDVPPPEVVHQEP"
     gene            complement(15787..16281)
                     /gene="rutF"
                     /locus_tag="ACHKTW_001693"
     CDS             complement(15787..16281)
                     /gene="rutF"
                     /locus_tag="ACHKTW_001693"
                     /EC_number="1.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent FMN reductase RutF"
                     /protein_id="HFO6462739.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHATPYGLVWFDRSYHALM
                     RPAC"
     gene            complement(16292..16882)
                     /gene="rutE"
                     /locus_tag="ACHKTW_001694"
     CDS             complement(16292..16882)
                     /gene="rutE"
                     /locus_tag="ACHKTW_001694"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HFO6462740.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWRETPVSDETLREIYALMKWGP
                     TSANCSPARIVFIRTAEGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPQLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQHVDDAFFA
                     GSTLKSNLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            complement(16892..17692)
                     /gene="rutD"
                     /locus_tag="ACHKTW_001695"
     CDS             complement(16892..17692)
                     /gene="rutD"
                     /locus_tag="ACHKTW_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HFO6462741.1"
                     /translation="MKLSLSPPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQVVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIEHYAVVGHALGALVGMQLALDYP
                     ASVTVLISVNGWLRINAHTRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAARAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPSACSS
                     ELHAALPDSQKMVMPYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            complement(17700..18086)
                     /gene="rutC"
                     /locus_tag="ACHKTW_001696"
     CDS             complement(17700..18086)
                     /gene="rutC"
                     /locus_tag="ACHKTW_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-aminoacrylate deaminase"
                     /protein_id="HFO6462742.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQHNNVLF
                     ADDPKAQTRHVLEIIRKVIETAGGTMADVTFNSIFITDWKNYAAINEIYAEFFPGDKP
                     ARFCIQCGLVKPDALVEIATIAHIAK"
     gene            complement(18098..18790)
                     /gene="rutB"
                     /locus_tag="ACHKTW_001697"
     CDS             complement(18098..18790)
                     /gene="rutB"
                     /locus_tag="ACHKTW_001697"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HFO6462743.1"
                     /translation="MTTLTARPEAITFDPQQSALIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
                     IATNVCVESTLRDGFFLEYFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFC
                     DALSPTSFARIA"
     gene            complement(18790..19881)
                     /gene="rutA"
                     /locus_tag="ACHKTW_001698"
     CDS             complement(18790..19881)
                     /gene="rutA"
                     /locus_tag="ACHKTW_001698"
                     /EC_number="1.14.99.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HFO6462744.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIVQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGTGKS
                     DFKGDFFTMNDCRVSPQPSVPMKVICAGQSDAGMAFSAQYADFNFCFGKGVNTPTAFA
                     PTAARMKQAAEQTGRDVGSYVLFMVIADETDDAARAKWEHYKAGADEEALSWLTEQSQ
                     KDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPGAEGVLLTFDDF
                     LSGIETFGERIQPLMQCRAHLPALTQEVA"
     gene            20169..20807
                     /gene="rutR"
                     /locus_tag="ACHKTW_001699"
     CDS             20169..20807
                     /gene="rutR"
                     /locus_tag="ACHKTW_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HFO6462745.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEVFFNQTVENVQRIIIEGIRPR"
     gene            complement(20847..24809)
                     /gene="putA"
                     /locus_tag="ACHKTW_001700"
     CDS             complement(20847..24809)
                     /gene="putA"
                     /locus_tag="ACHKTW_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0004657 - proline dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trifunctional transcriptional regulator/proline
                     dehydrogenase/L-glutamate gamma-semialdehyde
                     dehydrogenase"
                     /protein_id="HFO6462746.1"
                     /translation="MGTTTMGVKLDDATRERIKSAATRIDRTPHWLIKQAIFSYLEQL
                     ENSDTLPELPALLSGAANESDEAPTPAEEPHQPFLDFAEQILPQSVSRAAITAAYRRP
                     ETEAVSMLLEQARLPQPVAEQAHKLAYQLADKLRNQKNASGRAGMVQGLLQEFSLSSQ
                     EGVALMCLAEALLRIPDKATRDALIRDKISNGNWQSHIGRSPSLFVNAATWGLLFTGK
                     LVSTHNEASLSRSLNRIIGKSGEPLIRKGVDMAMRLMGEQFVTGETIAEALANARKLE
                     EKGFRYSYDMLGEAALTAADAQAYMVSYQQAIHAIGKASNGRGIYEGPGISIKLSALH
                     PRYSRAQYDRVMEELYPRLKSLTLLARQYDIGINIDAEEADRLEISLDLLEKLCFEPE
                     LAGWNGIGFVIQAYQKRCPLVIDYLIDLATRSRRRLMIRLVKGAYWDSEIKRAQMDGL
                     EGYPVYTRKVYTDVSYLACAKKLLAVPNLIYPQFATHNAHTLAAIYQLAGQNYYPGQY
                     EFQCLHGMGEPLYEQVTGKVADGKLNRPCRIYAPVGTHETLLAYLVRRLLENGANTSF
                     VNRIADTSLPLDELVADPVTAVEKLAQQEGQTGLPHPKIPLPRDLYGHGRDNSAGLDL
                     ANEHRLASLSSALLNSALQKWQALPMLEQPVAAGEMSPVINPAEPKDIVGFVREATPR
                     EVEQALESAVNNAPIWFATPPVERAAILHRAAVLMESQMQQLIGILVREAGKTFSNAI
                     AEVREAVDFLHYYAGQVRDDFANETHRPLGPVVCISPWNFPLAIFTGQIAAALAAGNS
                     VLAKPAEQTPLIAAQGIAILLEAGVPPGVVQLLPGQGETVGAQLTGDDRVRGVMFTGS
                     TEVATLLQRNIASRLDAQGRPIPLIAETGGMNAMIVDSSALTEQVVVDVLASAFDSAG
                     QRCSALRVLCLQDEIADHTLKMLRGAMAECRMGNPGRLTTDIGPVIDSEAKANIERHI
                     QTMRSKGRPVFQAVRENSEDAREWQSGTFVAPTLIELDDFAELQKEVFGPVLHVVRYN
                     RNQLPELIEQINASGYGLTLGVHTRIDETIAQVTGSAHVGNLYVNRNMVGAVVGVQPF
                     GGEGLSGTGPKAGGPLYLYRLLANRPESALAVTLARQDAEYPVDAQLKAALTQPLNAL
                     REWAANRPELQALCTQYGELAQAGTQRLLPGPTGERNTWTLLPRERVLCIADDEQDAL
                     TQLAAVLAVGSQVLWPDDALHRQLVKALPSAVSERIQLAKAENITAQPFDAVIFHGDS
                     DQLRALCEAVAARDGAIVSVQGFARGESNILLERLYIERSLSVNTAAAGGNASLMTIG
                     "
     gene            24864..25073
                     /locus_tag="ACHKTW_001701"
     CDS             24864..25073
                     /locus_tag="ACHKTW_001701"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6462747.1"
                     /translation="MMLQLCATMLNVTCVASLKMNEMLIKEIDMTGIKKITQTFSLRQ
                     LTFLKGATAKNVRECNLMKNSVAEH"
     gene            25232..26740
                     /gene="putP"
                     /locus_tag="ACHKTW_001702"
     CDS             25232..26740
                     /gene="putP"
                     /locus_tag="ACHKTW_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005298 - proline:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0015824 - proline transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HFO6462748.1"
                     /translation="MAISTPMLVTFCVYIFGMILIGFIAWRSTKNFDDYILGGRSLGP
                     FVTALSAGASDMSGWLLMGLPGAVFLSGISESWIAIGLTLGAWINWKLVAGRLRVHTE
                     YNNNALTLPDYFTGRFEDKSRILRIISALVILLFFTIYCASGIVAGARLFESTFGMSY
                     ETALWAGAAATILYTFIGGFLAVSWTDTVQASLMIFALILTPVIVIISVGGFGDSLEV
                     IKQKSIENVDMLKGLNFVAIISLMGWGLGYFGQPHILARFMAADSHHSIVHARRISMT
                     WMILCLAGAVAVGFFGIAYFNEHPAVAGAVNQNAERVFIELAQILFNPWIAGILLSAI
                     LAAVMSTLSCQLLVCSSAITEDLYKAFLRKHASQKELVWVGRVMVLVVALVAIALAAN
                     PENRVLGLVSYAWAGFGAAFGPVVLFSVMWSRMTRNGALAGMIIGALTVIVWKQFGWL
                     GLYEIIPGFIFGSIGIVVFSLLGKAPSAAMQKRFAEADAHYHSAPPSRLQES"
     gene            27283..28113
                     /gene="efeU"
                     /locus_tag="ACHKTW_001703"
     CDS             27283..28113
                     /gene="efeU"
                     /locus_tag="ACHKTW_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter permease EfeU"
                     /protein_id="HFO6462749.1"
                     /translation="MFVPFLIMLREGLEAALIVSLIASYLKRTQRGRWIGVMWIGVLL
                     AAALCLGLGIFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNVKVQLE
                     QAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAMLGLAT
                     AVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQEIAFD
                     MSAVLSTHSLFGTLMEGVFGYQEAPSVSEVAVWFIYLIPALVAFALPPRAGATASRSA
                     "
     gene            28171..29298
                     /gene="efeO"
                     /locus_tag="ACHKTW_001704"
     CDS             28171..29298
                     /gene="efeO"
                     /locus_tag="ACHKTW_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HFO6462750.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAANVPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDQYADQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFETYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            29304..30581
                     /gene="efeB"
                     /locus_tag="ACHKTW_001705"
     CDS             29304..30581
                     /gene="efeB"
                     /locus_tag="ACHKTW_001705"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0033212 - iron import into cell [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter
                     deferrochelatase/peroxidase subunit"
                     /protein_id="HFO6462751.1"
                     /translation="MQYEDKNGVNEPSRRRLLKGIGALALALAGSCPVAHAQKTQSAP
                     GTLSPDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKPDLERLFRLLTQRFAF
                     LTQGGAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQ
                     KMTRFPNDSLDAALCHGDVLLQICANTQDTVIHALRDIIKHTPDLLSVRWKREGFISD
                     HAARSKDKETPINLLGFKDGTANPDSQNDKLMQKVVWVTADQQEPAWTIGGSYQAVRL
                     IQFRVEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRL
                     ANPRTAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEA
                     LEEYVKPIGGGYFFALPGVKDANDYLGSALLRV"
     gene            31200..31988
                     /gene="phoH"
                     /locus_tag="ACHKTW_001706"
     CDS             31200..31988
                     /gene="phoH"
                     /locus_tag="ACHKTW_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HFO6462752.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMGGVEA
                     IGMARDSRDTSPILARNEAQLHYLKAIESKQLIFATGEAGCGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDVLVRRLGASFMQYCLRPEIG
                     KVEIAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVCSGLSDALERFEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
     gene            complement(32038..32451)
                     /gene="pgaD"
                     /locus_tag="ACHKTW_001707"
     CDS             complement(32038..32451)
                     /gene="pgaD"
                     /locus_tag="ACHKTW_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     biosynthesis protein PgaD"
                     /protein_id="HFO6462753.1"
                     /translation="MNNLIITTRQSPVRLLVDYVATTILWTLFALFIFLFAMDLLTGY
                     YWQSEARSRLQFYFLLAVANAVVLIVWALYNKLRFQKQQHHAAYQYTPQEYAESLAIP
                     DELYQQLQKSHRMSVHFTSQGQIKMVVSEKALVRA"
     gene            complement(32453..33778)
                     /gene="pgaC"
                     /locus_tag="ACHKTW_001708"
     CDS             complement(32453..33778)
                     /gene="pgaC"
                     /locus_tag="ACHKTW_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008375 - acetylglucosaminyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042710 - biofilm formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine synthase"
                     /protein_id="HFO6462754.1"
                     /translation="MINRIVSFFILCLVLCIPLCVAYFHSGELMMRFVFFWPFFMSIM
                     WIVGGVYFWVYRERHWPWGENAPAPQLKDNPSISIIIPCFNEEKNVEETIHAALAQRY
                     ENIEVIAVNDGSTDKTRAILDRMAAQIPHLRVIHLAQNQGKAIALKTGAAAAKSEYLV
                     CIDGDALLDRDAAAYIVEPMLYNPRVGAVTGNPRIRTRSTLVGKIQVGEYSSIIGLIK
                     RTQRIYGNVFTVSGVIAAFRRSALAEVGYWSDDMITEDIDISWKLQLNQWTIFYEPRA
                     LCWILMPETLKGLWKQRLRWAQGGAEVFLKNMTRLWRKENFRMWPLFFEYCLTTIWAF
                     TCLVGFIIYAVQLAGVPLNIELTHIAATHTAGILLCTLCLLQFIVSLMIENRYEHNLT
                     SSLFWIIWFPVIFWMLSLTTTLVSFTRVMLMPKKQRARWVSPDRGILRG"
     gene            complement(33771..35789)
                     /gene="pgaB"
                     /locus_tag="ACHKTW_001709"
     CDS             complement(33771..35789)
                     /gene="pgaB"
                     /locus_tag="ACHKTW_001709"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     N-deacetylase PgaB"
                     /protein_id="HFO6462755.1"
                     /translation="MLRNGNKYLLMLVSIIMLTACISQSRTSFIPPQDRESLLAEQPW
                     PHNGFVAISWHNVEDEAADQRFMSVRTSALREQFAWLRENGYQPVSIAQIREAHRGGK
                     PLPEKAVVLTFDDGYQSFYTRVFPILQAFQWPAVWAPVGSWVDTPADKQVKFGDELVD
                     REYFATWQQVREVARSRLVELASHTWNSHYGIQANATGSLLPVYVNRAYFTDHARYET
                     AAEYRERIRLDAVKMTEYLRTKVEVNPHVFVWPYGEANGIAIEELKKLGYDMFFTLES
                     GLANASQLDSIPRVLIANNPSLKEFAQQIITVQEKSPQRIMHIDLDYVYDENLQQMDR
                     NIDVLIQRVKDMQISTVYLQAFADPDGDGLVKEVWFPNRLLPMKADIFSRVAWQLRTR
                     SGVNIYAWMPVLSWDLDPTLTRVKYLPTGEKKAQIHPEQYHRLSPFDDRVRAQVGMLY
                     EDLAGHAAFDGILFHDDALLSDYEDASAPAITAYQQAGFSGSLSEIRQNPEQFKQWAR
                     FKSRALTDFTLELSARVKAIRGPHIKTARNIFALPVIQPESEAWFAQNYADFLKSYDW
                     TAIMAMPYLEGVAEKSADQWLIQLTNQIKNIPQAKDKSILELQAQNWQKNGQHQAISS
                     QQLAHWMSLLQLNGVKNYGYYPDNFLHNQPEIDLIRPEFSTAWYPKND"
     gene            complement(35798..38221)
                     /gene="pgaA"
                     /locus_tag="ACHKTW_001710"
     CDS             complement(35798..38221)
                     /gene="pgaA"
                     /locus_tag="ACHKTW_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1901515 -
                     poly-beta-1,6-N-acetyl-D-glucosamine transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6 N-acetyl-D-glucosamine export
                     porin PgaA"
                     /protein_id="HFO6462756.1"
                     /translation="MYSSSRKRCPKTKWALKLLTAAFLAASPAAKSAVNNAYDALIIE
                     ARKGNTQPALSWFALKSALSNNQIADWLQIALWAGQDKQVITVYNRYRHQQLPARGYA
                     AVAVAYRNLQQWQNSLTLWQKALSLEPQNKDYQRGQILTLADAGHYDTALVKLKQLNS
                     GAPDKANLLAEAYIYKLAGRHQDELRAMTESLPENASTQQYPTEYVQALRNNQLAAAI
                     DDANLTPDIRADIHAELVRLSFMPTRSESERYAIADRALAQYAALEILWHDNPDRTAQ
                     YQRIQVDHLGALLTRDRYKDVISHYQRLKKTGQIIPPWGQYWVASAYLKDHQPKKAQS
                     IMTELFYHKETIAPDLSDEELADLFYSHLESENYPGALTVTQHTINTSPPFLRLMGTP
                     TSIPNDTWLQGHSFLSTVAKYSNDLPQAEMTARELAYNAPGNQGLRIDYASVLQARGW
                     PRAAENELKKAEVIEPRNINLEVEQAWTALTLQEWQQAAVLTHDVVEREPQDPGVVRL
                     KRAVDVHNLAELRIAGSTGIDAEGPDSGKHDVDLTTIVYSPPLKDNWRGFAGFGYADG
                     QFSEGKGIVRDWLAGVEWRSRNIWLEAEYAERVFNHEHKPGARLSGWYDFNDNWRIGS
                     QLERLSHRVPLRAMKNGVTGNSAQAYVRWYQNERRKYGVSWAFTDFSDSNQRHEVSLE
                     GQERIWSSPYLIVDFLPSLYYEQNTEHDTPYYNPIKTFDIVPAFEASHLLWRSYENSW
                     EQIFSAGVGASWQKHYGTDVVTQLGYGQRISWNDVIDAGATLRWEKRPYDGDREHNLY
                     VEFDMTFRF"
BASE COUNT         9860 a         9206 c         9127 g        10211 t
ORIGIN      
        1 agcatttgct cattatttat tgttaatagt aatatggcat caatctcctt ggggaatgaa
       61 tccttttcaa cagatgacga ccttgagtat ggttatttaa taaacgctgg caatcattat
      121 gatgtttacc tccctcctga actttttgct caggcttata agttaaaaaa taaggaaatg
      181 aatgcgcaac tcgactattt aaatcgttat gcaatttaat ggcaaaggca tatgctaaaa
      241 accattgtta ttagtctcac acttttttat tggtaaatat tgtctctgta ttggtaacgc
      301 cgcagatatt ctgtttagcc acaggtgtaa tcatcagcgg cgtacgcgag gcagggagct
      361 aatcaggcat agtttgcgtc aaaccttgcc tgtttttaaa gatgtatata gaaaaacagg
      421 cattcaacaa gccattttgc gaacctgttc ccggaaaaat gtcatatttc tgtcacactc
      481 tttagtgatt gataacaaaa gaggtgccag gaatgaacaa aacgctaatc gccgcaactg
      541 tggcagggat agttttactc gcttcaaatg ctcaggcaca aaccgtaccg gaaggctatc
      601 agctacagca agtgctcatg atgagccgcc ataacttacg tgcgccgctg gcgaacaatg
      661 gcagtgtgct ggagcagtct acgccgaata aatggcccga atgggacgtc cccggtgggc
      721 aactcaccac caaaggtggc gtgctcgaag tgtatatggg ccattacatg cgtgaatggc
      781 tggcacagca ggggatggtg aaatcggggg aatgcccgcc gccggacacc gtttatgcct
      841 atgccaatag tctgcaacgt accgttgcga ccgcacagtt ctttattacc ggcgcattcc
      901 cggggtgtga tattcctgtg catcaccagg aaaaaatggg cactatggac ccaaccttta
      961 acccggtgat caccgatgat tccgccgcat tcagtgaaca ggcggtggcg gcaatggaga
     1021 aagagctcag caaactccag cttaccgaca gctaccagct actggaaaaa atcgttaact
     1081 ataaagattc ccctgcctgt aaagagaaac aacagtgttc gctggtggat ggcaaaaata
     1141 cctttagcgc caagtatcaa caagaaccag gtgtttccgg gccgctgaaa gtcggcaact
     1201 cgctggtaga tgcgtttact ttgcaatatt acgaaggttt tccgatggat caggtggcct
     1261 ggggagaaat caaatctgac aagcagtgga aggtgttgtc gaagcttaaa aacggctacc
     1321 aggacagcct gtttacctca ccggaagtgg cgcgcaatgt tgcgaaaccg ctggtcagtt
     1381 atatcgacaa agctctggtc accgatcgca ccagcgcacc gaaaattaca gtgttggttg
     1441 ggcacgactc caacattgcc tctctgttaa cggcgctgga tttcaaaccg tatcagttgc
     1501 atgaccagaa cgaacgcacg ccgattggcg gcaaaatcgt tttccagcgt tggcatgaca
     1561 gcaaagccaa tcgcgatttg atgaaaattg aatatgtgta tcagagtgcg gaacagttac
     1621 gtaatgccga tgcgttaacc ctgcaggcac ctgctcagcg tgtgacgctg gaattaagcg
     1681 gttgcccgat agatgccaac ggtttctgcc cgatggataa atttgatagc gtgttgaatg
     1741 aagcggtgaa ataacagaaa actcccccgc gagaagcggg ggagtcgctg gttaaacgtt
     1801 tttacgttcg atggtctgtt cgccccaaaa aagcgaatct ttatcggtct tagcaaaggc
     1861 tttgactaac acttcatcgc taccttcttc ccaaatcttt tccgccattt tttcgtcgta
     1921 cccggcgact tcgaaaatgg cctcggctat ttccggcgac gtattgcgca gagatgccca
     1981 ttcaccgacg tgatgagctt tcgcttcttg agttggcatg cgtatcctcc tgttgaagat
     2041 tagccgttaa gtttaactgc cagacctgcg acatattccc cttgataacg agcaatagac
     2101 agttcttcct ggcttggctg gcgtgagccg tcaccgcctg cgatggtggt tgcgccgtac
     2161 ggcgtaccgc cgcgaacctg tgtaacgtca aataattcct gcgctgcgta gccaatgggg
     2221 acaattacca tgccgtgatg cgcaagggtc gtccaggttg aagtaatagt ttgttcctga
     2281 ccgccgccag taccggtgga actaaagacg ctcgccagtt ttccgtatag tgcgccggaa
     2341 gcccacaggc cgcccgtctg gtcgaggaag gtacgcattt gaccggacat gttgccaaag
     2401 cgggtaggtg taccaaaaat aatggcgtcg taatcggcca gttcttgcgg ggttgcaacc
     2461 ggtgcagttt gcgttttacc gcctgctttt tcaaataatt gcggcggcat ggtttccggt
     2521 acacgcttaa cgacaacttc tgcgccatcg actttgcttg caccctcagc gactgcgcgt
     2581 gccatcgttt caatatgtcc gtacatggaa taataaagca ctagtattct tgccttaaca
     2641 ctcatatcat tgttctcctg ttcaatgaac cacttagata agcatagtcc gaagatatga
     2701 agttgctagc gacatacatt cgttggagta ctaaagagca agactctgga gactccttaa
     2761 gaagacaaac aaacctcatt gatgctttct actctaagca caaaaacgat tattaccttc
     2821 ttcctgctca cagatacgtt gataagggta aatctggttt tcaccagcaa cataaggctc
     2881 aaggttctga cttcagacgt atgtttgaaa atgttatgtc tggggttatt ccagaaggtt
     2941 ccctgattgt tgtagagaac tttgaccgct tcagtagggc agatatcgac acagctatag
     3001 atgacgttag gcagatactg aggaagggag tctctatact gactctggga gatggtgaac
     3061 tctacgacaa gtcagcctta acagaccctg tgaagttgat taagcacatc atcatagctg
     3121 agagagcaca tcaggaatct cttgttaagc aaaagcgtat agctcaggtc tggaaccata
     3181 aaacacagct tgcaagagaa cttaagaagc ctatgggtaa gcaagccccc ggatggttag
     3241 agctatcaga agacggttca cactacatcg ttgatgaaga taaagcgtca ctggtgaata
     3301 tcatctatga caagcgtctt tctggtatgt cgatgtttgc tatctgtaaa tggctaaacg
     3361 aacaaggtta cccgaccatt aatcagcgta aggtcagaat atccaagacc aagaaaccgg
     3421 atggcaactg gtcagcatta agcgttaaac acatcctgac ttcacgaagt gtattaggtt
     3481 accttcccgc taagatttct acagaagacc gtaaaacagt gttacgtgaa gaaattgaag
     3541 ggttctatcc tcagattgtc acagactcta agttttacgc tgtacagcaa ctgttagagg
     3601 aaacagggaa ggggaaaaca tccagcggtg accactggtt atacgtgaac atactgaagg
     3661 ggcttatccg ttgtaagtgt gggcttgtaa tgactcctac agggatacgt aaacccgttt
     3721 atcaaggtac ttaccgttgc aatggtaata aggagtcccg ttgtagctac ggtacagtgt
     3781 ccagaaagtt acttgataca cagctttgca gtcgtctgtt tagcaaacta tcacagctac
     3841 acgatgaagc tacggataca gctaaattag atgagctaca acgcagacta aacaccgttg
     3901 atagtgaact ggaaaaactc actgagacgc tgatacagct tcctaacatc actcagattc
     3961 aggaagcact acgggttaag caggaagaaa aagatgaact gatagttcag ttatcacgag
     4021 aaaaggcgag ggtaaaatct gtatcaagct tggatttaag cggtcttgat atggagtctg
     4081 tagaggggag aactgaagcc cagattatca ttaagcgtct ggtgaaagag attgttgttt
     4141 ctggtaatga aaagctggtg gatatatatc ttcacaatgg aaatatgatt cgtggtttcc
     4201 ctcttgatgg taaagatgac cacaccttaa cgcttgaaga agcaaccgat gagatgcagc
     4261 cattagatga tatgcttatc tttggtgaac cagtaacaag gatttatcct gctggtgata
     4321 tggaagaggt ggacgcttag gaattggtat tgttaccaat caataagtgc ccttcttatt
     4381 cggattcaca gagtactttc gtaaagcatt acccgactta agcacttttg aagcctgctc
     4441 attagctgag gctgtgaagg tgcttacacc atctttcacc gtgacatttt tagagcaatt
     4501 actacgggct tttaatgctt catcagatag ctcagacgct gagtaactgt acagttcctg
     4561 acacatcagc ataggtgcgt tattgcgttc agtagtcacg ttacccgcat tcttcttact
     4621 ggtagcacca ccatcattcg tggtagcggt ctttccagta gcagcagaaa tatcagtttg
     4681 ccctacaaca gctttgaagt taatttcctg aaaagttagc tggactctta gaccatttga
     4741 aataccaaca tctttagagg ctttaaaact tgtaataatg gaatcatcaa ttttaatacc
     4801 atctttacag atgaccgaaa taatttgttt ctggtctctc cagctttcaa gagtgtcaat
     4861 gaagttttct actaattgac cctgacgagt taataataag cttccttcat agccaactac
     4921 aacgacacca ctaatagtaa ttgttctggg tgctctttgc acattatctg tgacggtttg
     4981 ccctgattgc atgttctgtg tagtcacctg catagggctg tcaaattcca tgttttcagt
     5041 tgctgataag gttaagaagg catctacatt atctcttaag tggaagtaaa cgccatcttt
     5101 accactgtag cggatttgat tgctaaaaat cattaaattc tcctttgatt tcaaagactt
     5161 agtgtatttt aatcattttt aaccaagcct ttgatatata agtgttttct ctgttaagcg
     5221 tgagctacgt aaaaaataat ttttggtggg tatatctgtt cagaagattt cacgtagcac
     5281 acggatatat aagaaatcat tcttcctctg gatagcttga acttaagagc atatcctgcg
     5341 tgaactggtc attagctgat tgattgaaag taaagaaagc tggttcatcc ggtctaacag
     5401 ttgcttcacc agtgatatta actgacaaag gcttcttagc ttctgctgtt aaagcgtcta
     5461 cctttcttcc taactctgat acagccatag ctaattgagc attactatta accatgtcac
     5521 ctgtgatagc tggtggagtt gattgtgtgt tattaacaag gttactcatt ggctcttgga
     5581 agttagcaat tttattagct gcaccattaa accagtcagc gatagaacta ccaatcttag
     5641 cccatccctc attaatttgg tttggtttag atgaatcgcc tttgtattca gggttgaagt
     5701 ttgtctctgt tccaatacct gattggtgta agttcggatt cttagctgct tcagggttta
     5761 aattatcaag agcaaagttt accaactcag cataaaggac aactctaccg actttactta
     5821 atgcaccacc tgaagaacca gcctttccag ctttagcttt attaacagcg tcaccgaatg
     5881 aagtaacagc gtttgcaagg tttaacaagc ctccagctac atttgcagcc ttttgagcag
     5941 taacagctac ggcaagcatc ttcaagccat caattaaccc atcagataaa ccatcaacaa
     6001 tttttttctg agtgtcattt agtgaacgat aagccaatgt cacctcgtca aagaacgccc
     6061 ttaccctgta actaatatca gtcaggttat cgacaccatg tgacatatcg ctcatcaagc
     6121 taccaaaact ttcacctaat gttttaccac ttccaccaag agcgtttaaa agcgttgtta
     6181 ggcttcttgt gaaatcttgc agaccatttt cagaacctct gaagatacta tcaagcgtgg
     6241 tattccaaga gtttttcagg ttccctagct gtgcctgtgt actttctgaa atagctttcc
     6301 agccaccttt agcggtgatt tgtttattca gttcctgagt gaccttctga agagtatcgg
     6361 aagactttaa agcccccttc ttcatggcat caaataattc ctgttcactc tttccggttg
     6421 ctttagcgaa cacctgcacc ataccaggaa cagcttcagc cagttgcccc ttaagctctt
     6481 cagccataat ctgaccctta ccagccatct gtgataatgc tgtcagtgct cgcttaatct
     6541 cttcttcact tctccccatc agacgagcat agccaatcat atttttaaaa agttcttcag
     6601 aaccttgaat acccatagac tctttagctg aagcaccaaa acccgcaaac tgtgattgca
     6661 gttcagcgat atctaaaccc gttgtttgag ctaatgactt tgtgaactct tttacctgac
     6721 ctgcatcttt atcaaatacg aaatcaattg aacgttcagc acttgcacgc tgtaaaccaa
     6781 tttccataat cttctgataa ctggctaata cagcctgcat tgagataaat gcacctgctg
     6841 ttttaactgc tgctgaagac aggttatcaa gactattagc tgctctgtca ccattaatgc
     6901 tggtggatgt atgacctctg gctttagcaa ttgcttctct gactgaagcg tttaactgag
     6961 ctaacttctc attgaatttt gaagaatcca ttgacccggc tttagcagat agcccgtaaa
     7021 actgactctt aatgtatttc tcgacaattg ggttaaggcc ttttagcttg ctcatattcg
     7081 ctgagtaagc tgctgtgatt gctctgtttc cgctaattcg tggttgtctt ggttgttttg
     7141 gaggattaac agtgtcatta aatctggtgg taaactggaa agctttagta tttcttgctc
     7201 tctgttcaat cttgtcaaag gccttattaa acttcctttc aagctgccct aaagaagccg
     7261 ttaaattact atcaagctgc tttgtattaa tcctgaaacc tttatttagg ttcttttcaa
     7321 ttctttgtgc tgactgcaaa gctgcctttt caactctttg cagtccctta ataacgtttt
     7381 cattgaagcc tagctcaacg ataaaactat caactgtgaa tttgctcatt catgtttcac
     7441 ctcatagact tcggatagaa gactatcctc atatacaaaa aaaaggagag ttaccgatgt
     7501 cactcgataa tctctcccgt tgatttttac ccaaccaatt cttttttaat gtcggacaag
     7561 ataatgtaat tccgtttcac ctatgatttg agcatcagaa ggaatacggt caatacacca
     7621 ctttggaatc ttgcgtgttt ttggtttgtg tgaggtgatg taatgacggt tattcttaat
     7681 tcgtagcttt tgtctatttg gttgtagctg gagtctttcg caaacagacc gaatagtctc
     7741 taaacgttct tcaaacatta atcacctcac atattgcata atcaaacagc cttgctaaat
     7801 tctcgtggaa cactcagaaa atcattgaag ctgataatct gtgccaactc ttgacggtac
     7861 tccttagcca gcgcttgatg gtcactaatc agcttagcca gtcttgattt cagcaaatta
     7921 atctcatttt ctcttgcttt catcagtttc tctttggcaa tttcttcaag ctcagcttgt
     7981 gatttatcag caacaattag agacaatcga tgtggtgctc tgacagctct ttctgtacga
     8041 ataactcttt gaccttctgg taaatctgca atcactttac caatctctac agggtcatac
     8101 ccccaaccgt cacggtctag tcgagagaaa taagcataag aattggtttg aagctccatt
     8161 aaaatagcca tttcgtcaat ttcgtcttca agcttggact gaattttctc gtaaacactt
     8221 ctcattgctt cttgagagtc tttaatagcc tggaaatatc ggcgttgtag cttcaacttc
     8281 aaatcaagtt cacaccgttc gttcatagaa aaatcaattt caactcttgg aatctcgagt
     8341 ggttctggtg gaatatatgg acctttattt acttcgtgtg attcccgcca agctctatca
     8401 aaagattcac gcaaacttaa ttcgtgaata tcaagtgcac ttggtctgtt ctcaacaatc
     8461 atttcgtgtt cattacgaat agacattatt ttttcacctc tttggttttt gtttgtttgt
     8521 ttttcaattt atagaagcac actcagcaat cagaggtgaa gagtctgact gaacaagaac
     8581 ctgccaagtg tgcctttaaa aactgaaaaa gccctaaaca taggtgattc ggatattcat
     8641 ggaatcatcc ttatctgtga agggctttgg aggtttgctt tataacgtta acgaagtttc
     8701 aggaaaaact ttctttaaaa gctctgttta aaaactctta aagaaaaccc taatcatgag
     8761 ttagggcaaa gctgtaaagg gttcaatgaa tgattaagca ctttttgaga tagcctctca
     8821 gactaaactg aaaggccatg acgaaaagca ttgcaccttt ccaactcaga atatagttat
     8881 catcgatgta gagacgatta atagtttaat tcaaggagaa ttgctctcct catacttaaa
     8941 attatggcat atttaaggtt atttgtcaac agcttttaac agattggtaa catttatact
     9001 ttaatagatg cacctgcaat cataccaatc aatacaaaac agataatcac caattccatt
     9061 aatgcaccac cctcgaatcc gtcaccaaaa tattgtcagc gtacaaaatt gctatagccg
     9121 ccttgagata tttttcaggg ctaacgctgt atcggttagt ttcctcaaca ataaggcgct
     9181 tcatgtttga gtcggtaacg ggaaacataa aacgtggttc accagtttca attccctgaa
     9241 taaagtcttc aattgcttct gagttttcag ccattagttt taacagcctg tcaaaatcaa
     9301 tttccacacc tgcatcaatg aagctttgca ccatcatagc ggtgtcgtta tcaacgaaaa
     9361 tctgtaattc gtaattgctc atttttaaaa tctccttttt atcagacctc ctcaatcgct
     9421 ctgtaacgag ctaaatagtc tttttcaggg gtagcctgta ggggtgtact acatacccta
     9481 aaataagata gttttttgcg ttagaatgcg attgagagcg tctgagagtg ggtaaaaccg
     9541 ttttttgcct ttagtctgag actattaacc ccatctcact atgaaattga ctaaaccagc
     9601 tttttgttac tgaggttttt agtgtgaaag atggggattg cataatcaga tagatgccat
     9661 cattatcaat gtcgccccat ccactatgta catcaattag atttcgaaaa tatttaaagg
     9721 ctttgtctgt tttaagtttg agggtgaata ctttcttcat tctttccccc aattcttaat
     9781 gagttcatag acccgtttaa attgagtcgg gtcactataa tcaagctcga tatccagtga
     9841 ctttaattca tagcgtaact gctcaatttg atgtggcttt agtcttttct tgttacggtc
     9901 aagcgtaaca tcttcatatt cttttgagcg gctatcttgt ctgattagat gtgatgccag
     9961 tttataaaat ttgtatctag gtagcattaa atctccagct tatccgctaa cttgtgaatc
    10021 ttgatactta gtaaccttgc taaatccagt ccaaaagcat ttaaagattt cagactatca
    10081 aggtcatctt gcttaatcct gatatggtgt cttgcagcct ctaagcgact attaagcaaa
    10141 tcctgttcgt taatatcacg gttaattgca agcttcatca ctgctgagtg tggcagacct
    10201 gtaatgtttt tgattcggtt aagttcttca aaggctgtct ttgtaatacg ggtcaaagtg
    10261 tgtctgtttt tcattttaaa attcctctgc ttgtgcgtat acgtcaaatt caaagtctgg
    10321 ttcactggtt gtaaggctga tatttcccat ttccgcatga tgattgattg cctcaataac
    10381 tttcatgcaa atagtggcta agttagcgca atatttatca cttggtagct catggcttaa
    10441 tcgggttgct tgacgctgta gataacttgt tattgtgctt gagctaatct tgtcaatatt
    10501 tcgtagtaaa tagaaaactt ttcggtgata attatctctt ccggcattac ggcaagttcc
    10561 gataataata tcttcaattc gtgatggtaa ttgttcatag ctgtcaaaac tcatcaagaa
    10621 agtttttaaa gtattttctt tttctttgcg tactgacata aatctgtctc gtggcgtaat
    10681 gctacgaatg accgcttcaa ggttcttacg ttcggatttg ctaaaggttg gtacttcgta
    10741 gctggtgggg ggtgtctgtg tagctgtaac cgttgcttct gtttcttgct ttgctctctt
    10801 caagaaatct ttgaaattct ctgtcgttca ttcatattct gaccctcatg aagtgttatt
    10861 attctgttaa ctatatgtac aatgctagca ttttcaaaga ctaatgtcaa gcatttttta
    10921 acatttcatt aacatctgtg gtataatggg tttatgggga gagtaaaata taaaggcgtg
    10981 actatgaggt ttattcgata ggggttggta caagcccaac taaccgaatc aactatgtat
    11041 ttggtcgcat gttttgcatt gcacacagac ctcttcaatc gccctgtaac gagctaaaca
    11101 gcctcttcag gtcaactcat aaggtcaggt acttttttgt gtcagaatgc gattgagagc
    11161 gtcttacaag gaaaactaat ggtaaatctc cctcctgaac tggggtcacc tgatgtatgg
    11221 actggtggag gtgttgggct gattgcaggc ctgctacttc gatatttcat cgtcagagtg
    11281 tttgtcgtgt tcaaattgtt cggtcgtgaa tggtcgtttg acctcaagga caggaccaga
    11341 acagacaacc acacagaacc ccgctaaact cccttcaaaa cccctatacc atctcgtaca
    11401 ggggtatctt tagcattttg tgctatctat taaaaaacag cttgacagaa ccggaaagct
    11461 gtatctatca agtgttcacc tctctgacag taatttctaa aagttcttat atttcaataa
    11521 gttgtaatta gccatatata gagaactgac aagtctcttc acagttaact ctgtaacctt
    11581 aacagtaact tctaaaaaca cttatttttc aataagttgt aatcacccaa tataatagct
    11641 gttcagcttc ttcacagaga aacttcagag gtcttcttaa tcaatccact aagcgagaga
    11701 agagcgataa ccccagagag ggaagctcct ttcttaaccg acctcttaaa cgacctttac
    11761 agaccttgtt caaggtaatc acaatagatt tttcttccct cggtttcccg tgttcctcgg
    11821 tttgtctatt gagggctttg aaatgtccct gtaacttccc tcttagacag ggaagaataa
    11881 tattggtgat acaagcgaag cgcccctcta aaaagatttt ttagctattt tgtttaaaaa
    11941 catatcaaac gatacatttt attgatttag gtcaaagaca aaagcattga caagatggta
    12001 agattgttca catagccctc tcttaacaga ctttttaaga gcttttgaga agtttgttaa
    12061 acagacactc tttctagtgt tgtcttttac ttaagaggct tgtttaggta actctcaaga
    12121 catttcttaa gacttcatgt taagacagaa cctaccttcg gtttcatggt tctcttcctc
    12181 ccgttaaaca ccccttccta gcaacttcat acttatctgt caagtacctt tacagactct
    12241 atcaaaaccc tctaaatctg acctgatata gatgctgtga aggcatgttc tctaggtctg
    12301 tgtagcttac ttagtgggaa gagagggtga tacatgagaa agaaccctcc tgttaaaaag
    12361 atatctcttc ctcccgttaa acgctcttat caagccctct gcataatcac taccaaaaac
    12421 cttacaaggt aatatccagg cctcttatag ttgacctgat acagaagttt tacagacata
    12481 ctctctagat actatcaagg gacactaagc gagagagagg gtgatacatg agaaagaacc
    12541 ctcctgttaa aaagaccact tagaagtctg agactttagt aaaatctgtg aatataactt
    12601 ctcagacgct ctgtaacgta ttctaagcga ttttacccct ttattttaag gtgggtggta
    12661 catccctaca gattacccct aaaaatgtgg ttaaaactcg ctacagagct ttctatgaga
    12721 ttagcttcat gaagtaattc attactgaat cacctaatcg aatcaactat gaagcttaat
    12781 aggattgtgc aaaatctgta cagtagttat ttagcttctg aaaattttta taaaaatcgt
    12841 agtaccccct ctgcattccc cccttcaccg ataatcttaa aaaatatcta cactcaattg
    12901 attcggtaga atctacctca tgattgatgg ctgttaagta ccccagattc acgatatagc
    12961 accccctttg caaaaggaaa taaaatatct ataaccaact attgactttc ttcatgatat
    13021 ctgtttaccc ccacctcaag aaaccttcat cacacttcaa agcatctatc aagaagattt
    13081 cacctatcaa tagctgtaac attttatagc tctgtgaagt cctgcccagt tttctctgta
    13141 acagctacaa acttctgtaa ccttatatgt caatgactta gaagacatgc tctcaaggtg
    13201 ctgtatagcc tcttatcagc caatacacaa aacccttata aatcagtact gatacgtata
    13261 cagtgcgttt ttaacataat agctgtttta cgcactattc gtgttctaca tgaaatctgt
    13321 atagatatta aggtgtctgt aagtgatttt ttaagaggaa ctacgaagga actatgaggt
    13381 tgattcctag aacaaccgaa tcaactataa ccatcacttc ctaactttca cataggaaac
    13441 tattaattag cttgatagtc actttgaggg aatatgagat tagcttctaa tcgaatctaa
    13501 tattgatggt gaccaactat ctatatatcg ttatatagcc ctgtatatag cactatatgt
    13561 agtgtctgga gagggacgac taacacgata cagtttaccc tgaaaagaga cttcatagat
    13621 tctctatata tttcaatagg ttacgctaaa aagagactat atcgatactg catagaatct
    13681 gtgtagaatt gcatagatac ttgggaaaat gcctttaaat tgaatagaga ttcacctgat
    13741 attgtcctgt ttttacaggt agatgaatgt acgtgtttag ctatgtaaaa gcaccagaac
    13801 tttagccatt tctaaccact cctcgtgtta tctctattcc gtagcgattc gctaccactt
    13861 atttaaagat aagacgtcct tttcagagtg caaatttcac aaccacttat ttgatttata
    13921 acaactttca caagcacgta attttgtcgc aaaatgacac atttttatct catcgcgttt
    13981 ttttaatcat aagagcggct tatggataat tatttgagat gatatctatt ctcgctaaga
    14041 agctgttgca ggatattacc aaacgcgggt ctgcccgcgt cagttcacta agcttagtcc
    14101 cacgtagcga aaatatggca gccgccatac gccgcgttaa ttctatgcaa tatgatgtct
    14161 atacccagac ggaggtcagt aatggcaaac catcgaggcg gttccggcaa ttttgcagaa
    14221 gaccgcgaaa gagcatcaga agcaggtaaa aaaggtggac agcacagcgg gggtaatttc
    14281 aagaatgacc cgcagcgcgc atctgaagca ggtaaaaaag gtggcaagag cagtcacggc
    14341 aaaagcgaca actagccggg ctaatcaatg acgaatgcat ttttttctta ggctcgtcaa
    14401 aaagtcatca ccgccggtaa tcccggcggt tttctgttta tggctcctga tgaacaactt
    14461 ctggcggtgg aacgtcaacc aactttctgc ttaacaacgc attgagtaaa atcgcgccaa
    14521 aggttgctgt accaatccct cccaacgtaa aaccgcccag cgtgagagca aaatcacccg
    14581 cgcccagcac taaggttact gcgaccataa tcaaattacc gttctggctt aaatcgacac
    14641 ggttttgtac ccatatcctt gcgcctgcga cggcaatcag cccgaacaca acaattgatg
    14701 caccaccaat caccgcggcc ggaatggtat gaatcagcgc gccaaatttc ggtgaaaagc
    14761 ccaacagcat ggcgatgacg gcagcagcga caaacaccag cgtcgagtag acttttgtca
    14821 cggccattac accgatattc tcggcgtagg tggttacgcc gctgccaccg acagaaccag
    14881 aaagcatcgt cgccagccca tcaccgacga atgcccgccc catatacggg tccatattgc
    14941 gtccggtcat cccggcgacc gccttgagat gacctaagtt ttccgccacc agaatcaccg
    15001 ccacgggcgc aatcagcatc attgcctgac tattaaaagc aggagtggaa aaatgtggca
    15061 aaccgaacca ggcagcatgg ctgacgagag taaaatcgac ggcttttccc agccctaaaa
    15121 ggttggtcat cacgccatac agcagacagg cgacaattaa acctacgaga atcaataacc
    15181 gctggatcat gccacgggta aacaccgcca ccagcccaat acacagcacc gtcattaccg
    15241 ccatccagct atcaaaggcc gaagccgata cacttttcac tgcgataggc gctaagttca
    15301 ggccaatcgc catcaccacc gcacccgtca ccaccggcgg cattagtcgt tcaatccagc
    15361 gtgtaccaat tttcatcacc accaggccaa tgacggtata aaccagccca caggcgataa
    15421 tcccgcccag cgcaatgctg atattcgggt taatgccctg accgttaaag cccgtcgcgg
    15481 caatcaccac gccgacaaaa gccgcgctgg aaccgagata actggggacg cgcccgccgg
    15541 taataaagaa aaacagtaac gtgccgatcc ccgacattaa aatggaaaga ttgggatcca
    15601 gccccatcag aatcggcatt aacaccgtcg cgccaaacat cgccaccgcg tgttgaacgc
    15661 ccattactgc cgtctgagca aacggcaatc gttcatacgg cgcgaccacg ccgctctctg
    15721 tagaggtcga ttttaactgc cagtgaggaa aaccgaacat tgccatcagc tgtctccttt
    15781 aggaggttaa caagcagggc gcatcagcgc gtgataactg cgatcgaacc acaccagccc
    15841 gtagggtgtg gcgtgacgat gaatcgcttc gatggcgcaa aacagaatgt cgtgggtgcc
    15901 gacgctcacc acctggctga tacggcagtc aaacgaaacc agagcctctt ccagttgcgg
    15961 gcatccggtc acacccgtct gccagcgggc ggcggcaaag cggtgttcca tgggcgtttt
    16021 gccgccaaaa agatttgaaa gcggctcctg cccggcgctc agtgtattta cacacagcgt
    16081 tcgattttca ttgaataccg gccagacgga cgccccacga ttcaggcaca ccagtaatgt
    16141 aggcggcgtg tcggtcacgc tgcagaccgc gctggcggtg aacccggcgc gcccggctgg
    16201 accatccgtg gtgatgatat tgactgccgc gcccatacag gacatcgcat cgcgaaaggt
    16261 ttgttgatcg acaatgttca tggtttgctc cttacaacag cccgcaggct tcttcaaagg
    16321 acagacgtgg caggcgcgca aaaagtttgc tgctatcgcc atagccgata ttaatcagca
    16381 gattgctctt cagcgtgctg cccgcaaaaa aggcgtcgtc aacgtgttga cggtcaaaac
    16441 ccgacatcgg gccggtatcc agtcccagcg cacggcaggc gacgatcaga taggccgcct
    16501 gcatggaact gttgcgaaac gctgtttctt cggcaagttg tgggctggag gtaaaccaac
    16561 tgcgggcatc gccgtgagga aacagttgcg gtaaccgttc ataaaattca ctgtcccagg
    16621 cgacgatagc ggtgacgggc gcggtcaggg ttttttgcag attgccgctg gaaagtgccg
    16681 ggcgcagacg ttcttttcct tctgccgtgc ggataaacac gatccgtgcc ggagaacagt
    16741 tagctgatgt cggcccccat ttcatcaggg cataaatctc ccgtaacgtc tcatcgctga
    16801 cgggtgtctc ccgccagccg ttgtgagtgc gggcatcggt gaacaaggtg ctaagcgcac
    16861 ctgggctaac ggcttcgttc atagcaattc cttacagggc ggcttcacgg tgatgtaaca
    16921 ggctggcaag cccgttgagt aacagagcat taaacgtttc gggatcggtc acgttgcagg
    16981 cgtgtccgcc atagggcatc accattttct ggctatcggg cagggcggca tgaagctcac
    17041 tggaacatgc tgatggcacc agcagatcat cactggcgca gatgatttgc accgggcagc
    17101 ggatgcgatc cgcatggtga ctaaagtcag cgcgtttgag ggcgttaagt cgacgcagta
    17161 aattattttt gccctgaaaa tgcgccagtg ccagcgcgtc ttctgcctcc aggcgaggtg
    17221 cgcgggccgc catccagtcg gcgggataga ggaacaacgg ctgcgcttcc acccatgcct
    17281 gcgcgccgcc gctatacagt aatcgttcgc gaacctgaaa acagcggcgc gtatgggcgt
    17341 ttattcgtag ccagccgtta acgctgatca gcacagttac cgacgcggga taatccagcg
    17401 ccagctgcat tcccaccagc gcaccgagcg catggccgac caccgcgtaa tgctcaatcc
    17461 ctgcggctac cagcgcctga tgcagttccg ctgccatctg ggcgatactg taatcttctg
    17521 ccagcgtgtc gggattattg ccggtgccgc gctggtcgta acagactacc tgatactcct
    17581 gctccagcac cgccagttgc ggtaaccagt aactgccgct accgccaaga cccgaaatca
    17641 acaccactac gggcgcatca gcataagggg gaggtgagag tgaaagtttc atcgcggcct
    17701 cacttggcga tatgcgcaat tgtggcgatt tccaccagcg cgtcaggttt taccagtccg
    17761 cactgaatgc agaatcgcgc cggtttatca cccggaaaaa actcggcgta gatttcgtta
    17821 atcgcggcgt aatttttcca gtcggtaata aagatgctgt tgaaggtcac atccgccatc
    17881 gtgccacccg ccgtctcgat caccttgcgg ataatttcca gaacgtggcg ggtttgcgcc
    17941 tttgggtcat cggcaaacag cacgttatta tgttgatcaa aagccagcgt accggagaca
    18001 tacaccacgc catcagccag cgtgccggga acgaaggggg ccagcggtgc gctgctgcca
    18061 gcgggaataa ttacggattt tggcatcgtt aaactcctta agcgatacga gcaaaggatg
    18121 tgggagaaag cgcgtcgcag aacgtttcga cgtcgctgac ccagccaaaa aaggtttcga
    18181 tattgaacaa cgcggctttc tgtgcaaatt ccggccccgc ctggtgtgtt gcgtcttcca
    18241 gcaccacgcc gaaatactcc agaaaaaagc catcgcgtag cgtcgattcg acgcagacgt
    18301 tggtagcgat accggtgaaa accagatggc gtattccacg gctgcgcaaa atgctgtcca
    18361 gcggtgtatt gaagaaaccg ctgtagcgcg gcttcggcag cacaatatcg ccaggctgcg
    18421 gcaccagttc atccaccagt tgataatccc aggagccttt cgccagcaat ttcccctgca
    18481 gctgcggctg cttacgcatg gttttcaggg cgttcgattt atgaaaattc ggtgagccgg
    18541 ggccgccagc ttcgacatac tgttcatccc agccattttg aaaccagatg atcagcatcc
    18601 ctgccgctcg cgctgcggtc acggcggttt gaatgttggc aatgaccggg cgagtggttg
    18661 agacatcaaa cccggcgaga tctaagtagc cgcctggcgt ggcataagcg ttttgcatat
    18721 ccaccacgat cagcgcactt tgctgcggat cgaaggtaat ggcttccggt cgagcggtta
    18781 aggttgtcat catgccacct cctgagtcag cgcaggtaga tgggcgcggc actgcatcag
    18841 tggttgaatg cgctcgccga aggtttcgat tcccgacaga aaatcatcga aggtcaacag
    18901 cacgccttcg gcaccaggca cgcttgcgac ttcatctaac atgcgcgcga cactggcgta
    18961 agaaccgact aacgtcccca tattgatgtt taccgccgaa gtgggatcgg ccatctgacg
    19021 gacgttggta tcagtaccgg agcgggtatc tttctgactt tgttcggtta gccagcttaa
    19081 cgcctcttca tccgcgcccg ctttgtagtg ttcccatttg gcgcgagcgg catcgtcggt
    19141 ttcatcggca atcaccataa acaacacata agaaccaacg tcgcgcccgg tttgctctgc
    19201 ggcctgtttc atccgcgcag cggtcggggc gaaagccgtg ggcgtattta cgcctttgcc
    19261 gaaacagaag ttgaaatcgg catactgggc ggagaacgcc atgccagcgt cgctttgccc
    19321 ggcgcagatc actttcatgg ggacactcgg ttgcggactg acgcgacaat cattcatggt
    19381 gaaaaaatcg cctttaaaat cgcttttccc cgtgccccac aggtcgcgca gcacctgaac
    19441 atattcggtg agatagtcgt aacgacggga gaaatagtca tcgccaggcc agatacccat
    19501 ctgctcatac tcgggctttt gccagccagt cacgaggttg acgccaaaac gcccgccaga
    19561 gatggagtcg atggttgcgg ccatacgggc gacgattgcc ggaggtaacg ttaaggtggc
    19621 agcagtggcg taaatctgaa tgcgcgaggt gacggccgcc agccccgcca tcaaggtgaa
    19681 cgactcaagg ttgtgatccc agaactcagt tttgccgcca aagccacgca gtttgatcat
    19741 cgacagggcg aaatcgaaat ggtagtgctc cgctttttgc acaatggctt tattcagttc
    19801 aaaggtcggc atgtactgcg gcgcgtgggt cgaaatgagc cagccgttgt tgccaatagg
    19861 tacgaatacg ccaattttca tcatcaacct ctcttcgtct cgtaaagtga aagtcagacg
    19921 gggcgctgca tcctgcatat ccttttcagc cgcgtaatgg cttgtttgca aagcggatgc
    19981 cagtttttaa aaagtgaatg ttattaatgt gttaacattg tgttgtctaa aatatctggt
    20041 aaaaagtgga ctaaacggtc aaaacagttg cacataaaac atgcatctgt gcgcgatgag
    20101 agtgcagaag gtcgaggccg ggcgggggtt ttgctatcct gttgccaatc tacaagaggg
    20161 gagagcgcat gacgcaaggc gcagtgaaaa caacgggtaa acgttcgcgc gcagtaagcg
    20221 cgaagaaaaa agcgattctt agcgcagcac tggacacttt ttcacaattc ggttttcacg
    20281 gcacaaggct ggagcagatc gcagagttgg cgggtgtttc aaaaaccaat ctgctgtatt
    20341 actttccgtc aaaagaggcg ctgtatattg ccgtgctgcg gcagattctc gatatctggc
    20401 tggcaccgtt aaaagcgttt cgtgaagatt tcgctccgct ggcggcgata aaagagtaca
    20461 tccgtctgaa gctggaagtc tcccgcgatt atccgcaggc ttcgcgcctg ttctgtatgg
    20521 agatgctggc aggcgcgccg ctgttaatgg atgaactgac gggcgatttg aaggcattaa
    20581 ttgatgagaa atcggcgctg attgccggtt gggtcaaaag cggcaaactc gcgccgattg
    20641 atccgcagca tttgattttc atgatttggg cttccactca acattacgcc gatttcgccc
    20701 ctcaggtgga ggcggtgaca ggcgcgacgt tgcgcgatga ggtatttttc aatcaaacgg
    20761 ttgaaaacgt gcagcggatt attattgagg ggattcgacc acgttaaaga tgccggagga
    20821 ggttgtaaca tcctccggct acctgtttaa ccgatcgtca ttaaactggc gttaccgcca
    20881 gcggcagcgg tattcacact cagcgaacgt tcgatataca gccgttccag aaggatattg
    20941 ctttcgccac gggcaaaacc ctgcaccgaa acaattgcgc catcccgcgc agcaacggct
    21001 tcacacaatg cgcgaagctg atccgaatca ccgtggaaga tcaccgcatc aaagggttga
    21061 gcggttatat tttccgcttt cgccagttga atacgttcgc tgactgccga tggcaatgcc
    21121 tttactagct gacgatgcag cgcgtcatcc ggccacagta cctggctgcc caccgccagc
    21181 acggcggcga gctgagtcag cgcatcctgc tcatcatcgg caatacacaa cacgcgctca
    21241 cgcggcagca gcgtccaggt gttgcgttca cccgtcggcc ccggcagcaa tcgttgtgtt
    21301 cctgcctgcg ccagctcgcc atattgcgta cataacgcct gcaattctgg acgatttgct
    21361 gcccattccc gcagtgcatt tagcggctga gtcaatgcgg ctttcaactg tgcatcgacc
    21421 ggatactctg catcctgacg cgcgagcgtc actgccagcg cactttccgg gcgattcgcc
    21481 agcagacggt agagatagag cggaccgcct gctttcggcc cggtaccgga caacccttcg
    21541 ccgccgaatg gctgcacacc caccactgcg cccaccatat tacggttaac atacaggtta
    21601 ccaacatggg ccgagccagt gacctgggcg atggtttcat caatacgcgt atggacacca
    21661 agcgtcagac catagccgga agcgttaatc tgctcgatca gctctggtag ctggttacgg
    21721 ttgtaacgca ccacatgcag caccggacca aagacctctt tttgcaattc ggcaaagtca
    21781 tccagttcga tcagcgtcgg ggcgacaaag gtgccgcttt gccattcacg ggcatcttcg
    21841 ctgttttccc gcaccgcctg gaacaccgga cggcctttgc tacgcatggt ctgaatatgg
    21901 cgctcaatat tggctttcgc ttcgctatca atcaccggac cgatatcggt ggtcaggcga
    21961 cccggattac ccatccggca ttcggccatt gcgccgcgca gcattttcaa cgtgtggtcg
    22021 gcaatctcat cttgcaggca cagcacgcgc agcgccgaac aacgctgacc cgcactgtcg
    22081 aacgccgagg ccagtacatc cacgacgacc tgttcggtca gtgctgaaga atcgacaatc
    22141 atcgcgttca tgccgccggt ttcagcgatg agcggaatag ggcgaccctg agcgtccagg
    22201 cggctggcga tattgcgctg cagtaacgta gcgacttcgg ttgaaccggt aaacatcacc
    22261 ccgcgtacgc gatcatcacc cgtcagttgc gcgcccacgg tttccccctg acctggcagc
    22321 aactgcacca cgcctggcgg tacacccgct tccagcaaaa tggcgatccc ttgcgcggca
    22381 atcagcggcg tttgttctgc cggttttgcc agcacgctgt tacctgccgc cagtgcggca
    22441 gcaatctgcc cggtgaaaat agccagcggg aagttccacg gactgataca caccacaggc
    22501 cctaatggac ggtgggtttc gttagcgaaa tcatcccgca cctgtccggc atagtagtgg
    22561 agaaaatcga ccgcttcgcg cacttcggca atggcgttac tgaaggtttt tccggcttca
    22621 cgcaccagaa taccaatcag ttgctgcatc tggctttcca ttagcacggc agcgcgatgc
    22681 aaaatcgccg cgcgttcaac cggaggagtg gcaaaccaga ttggcgcgtt attaaccgca
    22741 ctttccagcg cctgttctac ctcacgcggc gtggcttcac gcacaaagcc cacaatatct
    22801 ttcggttccg cagggttaat aacgggcgac atctcacctg ccgctaccgg ttgttccagc
    22861 attggcaagg cctgccattt ttgcagtgca ctattgagca gggcagaaga gagcgaggcc
    22921 agccggtgtt cgttagcgag atccagccct gccgagttgt cgcgcccgtg accgtaaaga
    22981 tcgcgcggca ggggaatttt cggatgcggt aatccagttt gcccttcctg ttgcgccagt
    23041 ttttctacag cagtgaccgg atcggcgacc agttcatcca gtggcaaaga ggtgtcggca
    23101 atacggttaa caaacgaggt gttagcaccg ttttccagca ggcgacgcac cagatacgcc
    23161 aacagcgttt catgggtacc gaccggagca taaatacgac acggacggtt aagtttgccg
    23221 tcggcaactt tcccggtgac ctgctcatac agtggctcgc ccataccatg caggcactgg
    23281 aactcgtact gacccggata gtagttctga cctgccagtt gataaatcgc tgccagcgta
    23341 tgggcgttgt gcgtcgcgaa ctgcgggtag attagattcg gcaccgccag cagctttttc
    23401 gcacaggcga gatatgaaac gtcggtatac accttgcggg tataaaccgg atagccttca
    23461 aggccgtcca tctgcgcacg cttgatttca ctatcccagt acgcgccttt caccaggcga
    23521 atcatcagac ggcgacggct gcgggtggcg agatcaatca ggtaatcaat caccagtggg
    23581 cagcgttttt gataagcctg aataacaaaa ccgataccgt tccagcctgc cagctcaggc
    23641 tcgaaacaga gtttttccag cagatcgagg gagatctcca gacgatcggc ctcttcggcg
    23701 tcaatgttga taccaatatc atactgacgc gccagcaggg tgagtgattt cagacgtggg
    23761 taaagctctt ccattacccg gtcatactgg gcgcggctgt agcgcggatg cagcgctgac
    23821 agtttgattg aaatgcccgg accttcatag atgccacgac cgttagacgc tttaccgatg
    23881 gcgtgaatcg cctgctgata ggaaaccata tacgcctgtg catctgcggc ggtcagcgcg
    23941 gcttcgccca gcatatcgta agagtaacgg aaacctttct cttccagctt gcgggcattg
    24001 gctaacgctt ccgcgatggt ttcgccagtg acgaactgct cgcccatcag gcgcatcgcc
    24061 atatccacac ctttgcggat cagcggttca ccgcttttac cgataatgcg gttcagcgag
    24121 cgggagaggc tggcttcgtt atgggtggaa accagtttgc cagtaaacag cagcccccag
    24181 gtagcggcat taacaaacag ggacgggcta cgaccaatgt gtgactgcca gttaccgttg
    24241 ctgattttgt cgcgaattaa cgcgtcgcgg gtggctttgt cgggaatacg caacaacgct
    24301 tctgccagac acatcagcgc cacgccttcc tgcgatgaca gcgaaaactc ctgcaataac
    24361 ccctggacca tacctgcgcg accactggca tttttttgat tacgcagttt atcggccagt
    24421 tgatacgcca gtttgtgcgc ctgttcagca actggctgcg gcaggcgagc ttgttccaac
    24481 agcatagaaa ccgcttcggt ttccgggcgg cgataggccg cggtgatcgc ggcgcgggaa
    24541 accgactggg gcaatatttg ctcggcaaag tcgaggaaag gttggtgtgg ttcctctgcc
    24601 ggagtcggtg cttcatcgct ctcattggca gcgccagaaa gcagcgcagg tagctccggc
    24661 agagtatcgc tgttttccag ttgttcgaga taagaaaaaa tcgcctgctt aattaaccag
    24721 tgtggtgtgc gatcgatacg tgtcgcggca gacttaatac gctcacgcgt cgcgtcgtcc
    24781 agcttaaccc ccatggtggt ggttcccatg ccattactcc tgttgttctg aaaggtgcaa
    24841 cttaacgtta tcgtgaaata tccatgatgt tgcaactttg tgcaaccatg ttaaatgtga
    24901 catgcgtagc aagcttaaaa atgaatgaaa tgttaataaa agaaatcgat atgacaggga
    24961 ttaaaaaaat aactcagact ttttctctgc ggcagttaac atttttgaaa ggtgcaaccg
    25021 caaaaaatgt gagagagtgc aacctgatga aaaatagtgt cgctgagcac taaaatttaa
    25081 tgtaaatggt gtgttaaatc gattgtgaat aaccagcgct tccggcagga tacggtcgcc
    25141 ctggtaaaac ataaactctg ttaccccgtt ccggtggcag atataacggc aagtttcgac
    25201 attgccgata ataatttttt ggagacttta gatggctatt agcacaccga tgttggtgac
    25261 attttgtgtc tatatctttg gcatgatatt gattgggttt atcgcctggc gatcaacgaa
    25321 aaacttcgac gactatattc tgggcggtcg tagccttggg ccattcgtga cggcattatc
    25381 ggcgggtgcg tctgatatga gcggctggct gttaatgggg ttgccgggcg cggtttttct
    25441 ttccgggatt tccgaaagct ggatcgccat tggcctgaca ttaggcgcgt ggatcaactg
    25501 gaagctggtg gccgggcggt tgcgtgtgca taccgaatac aacaataacg ccttaacatt
    25561 gccggattat ttcaccgggc gctttgaaga taaaagccgc attttgcgca ttatctctgc
    25621 gctggttatt ttgctgttct tcaccattta ttgcgcttcg ggcattgtgg caggcgcgcg
    25681 tctgtttgaa agtacctttg gcatgagcta cgaaacggct ctgtgggcgg gcgctgcggc
    25741 gacgatcctt tacaccttta ttggcggttt cctcgcggtg agctggactg acactgtaca
    25801 ggccagcctg atgatttttg ccctgatcct gacgccggtt atcgtcatta tcagtgtcgg
    25861 tggctttggt gactcgctgg aagtgatcaa acaaaagagc atcgaaaacg tggatatgct
    25921 caaagggctg aactttgttg ccattatctc attgatgggt tgggggctgg gttacttcgg
    25981 tcagcctcat attctggcgc gttttatggc ggcggattct caccacagca ttgtccatgc
    26041 gcgtcgtatc agtatgacct ggatgatcct ctgcctggca ggggcggtgg ctgtcggctt
    26101 ctttgggatt gcttacttta atgagcaccc ggcggtagca ggtgcggtaa accagaacgc
    26161 tgaacgtgtg tttatcgaac tggcgcaaat tctgtttaac ccgtggattg ccgggattct
    26221 gctgtcggca attctggcgg cggtaatgtc aacgttaagc tgccagctgc tggtgtgctc
    26281 cagtgcgatt accgaagatt tatacaaagc gtttctgcgt aaacatgcca gccagaaaga
    26341 gctggtgtgg gtagggcgtg tgatggtgct ggtggtggcg ctggtggcga ttgcgctggc
    26401 ggcaaacccg gaaaaccgcg tgctgggctt agtgagctac gcgtgggcag gctttggcgc
    26461 ggcgtttggt ccggtggtgc tgttctcggt gatgtggtca cgcatgacgc gcaacggtgc
    26521 gctggcgggg atgatcatcg gtgcgctgac ggttatcgtc tggaagcagt tcggctggct
    26581 gggactctac gaaattattc cgggctttat cttcggcagt attgggattg tagtgtttag
    26641 tttgctgggt aaagcgccgt cagcggcgat gcaaaaacgc tttgccgagg ccgatgcgca
    26701 ctatcattcg gctccgccgt cacggttgca ggaaagctaa gggacttagc ctgcggcggt
    26761 tttgtttggc ttcagcagcg ggttgcgctc ccttaatgtg cctcgccata taaattgaat
    26821 ggtgcaggga gcgcgcaggg ggcggccaat cgccgccgcc ccctgctgtc ccggccttcg
    26881 gggaacgctt cagcgatttt gacgccacca acacccgagc tgttattatg ttccgggcaa
    26941 aaagttagat ttgataatcg cggatggacg aaattgcttg atacacccgc ttatcagttt
    27001 tacatggaag ctctgatgca ttgagtctgg acagttttgt cggctggata cggcgtttac
    27061 gcggcatccg gcaagaacac atggttcttt gcaaacaatc ccatctttct accctggaat
    27121 aatcgtttat atcccttggc attacctctc tttgtttaca ttccaacatc attttataaa
    27181 cattccgctt gtatttttct ttaccgtaat gataatcgct atcactgcga tttacttttc
    27241 tttgcataga ttgactcaga aaaacgttta agggtgggtg gcatgtttgt tccgtttctc
    27301 attatgttgc gcgaaggact tgaagccgcg ctgattgtca gtttgattgc cagctatctt
    27361 aagcgtaccc agcgaggccg atggattggt gtgatgtgga ttggcgtgtt gcttgccgct
    27421 gcgttgtgcc tgggcctggg tatcttcatt aacgaaacca ccggcgaatt tccgcaaaaa
    27481 gaacaggaac tgtttgaagg tatcgtggcg gtgatcgccg tggtgatcct tacctggatg
    27541 gttttctgga tgcgcaaagt gtcgcgcaac gtcaaagtgc aactggaaca ggcagtcgat
    27601 agcgcattgc agcgtggaaa tcatcatggc tgggcgctgg tgatgatggt cttttttgcc
    27661 gttgcaaggg aagggctgga gtcggtcttt ttcctgctgg cggcatttca acaagatgtc
    27721 gggatctggc cgccgctggg tgcaatgctc ggtcttgcta ctgccgtggt gctcggcttc
    27781 ctgctctact ggggcggtat tcgcctcaat cttggtgcat tttttaaatg gaccagcctg
    27841 tttattctct tcgtcgccgc agggctggcg gctggtgcca ttcgcgcatt tcatgaagcc
    27901 ggattgtgga accactttca ggaaatcgcc ttcgatatga gcgcggtcct ctcaactcac
    27961 tcgctgtttg gcacgctgat ggaaggggtt tttggctatc aggaagcacc gagcgtcagc
    28021 gaagtcgccg tctggtttat ttatctcatc ccggcgctgg tggcatttgc cctgccacca
    28081 cgcgcagggg cgacagcgtc tcgctccgcg tagcaaatac gacgcaaact cttgcttagt
    28141 tacaacatac tttaaaggga tagtctcgtc atgaccatta acttccgccg taacgcattg
    28201 cagttgagcg tggctgcgct gttctcttct gcttttatgg ctaacgccgc taatgtgccg
    28261 caggtcaagg tgaccgtgac cgataagcag tgcgaaccga tgaccattac ggttaacgcc
    28321 gggaaaacac agttcattat tcagaaccac agccagaagg cgctggagtg ggagatcctc
    28381 aaaggcgtga tggtggtgga agagcgggaa aatatcgccc ctggctttag ccagaaaatg
    28441 acggcgaatt tacagcctgg cgaatacgat atgacctgcg gtctgctgac taacccgaaa
    28501 gggaagttga tcgtcaaagg tgaggcaacg gcggatgcgg cgcaaagtga tgcgctgtta
    28561 agtcttggtg gtgcaattac tgcatataaa gcgtatgtca tggcggaaac cacgcagctg
    28621 gtgaccgaca cgaaagcctt tactgacgct attaaagcag gcgatatcga aaaagcgaaa
    28681 gcactgtatg cgccgacgcg ccagcactat gagcgcatcg aaccgattgc tgaactgttc
    28741 tccgatctgg atggcagcat tgacgcccgt gaagatgatt acgagcaaaa agccgccgat
    28801 ccgaaattca ccggtttcca ccgtctggaa aaagcattgt ttggcgacaa caccaccaaa
    28861 ggcatggatc agtacgctga ccagctttat accgatgtgg tcgatttgca aaaacgcatc
    28921 agtgaactgg ctttcccacc ttcaaaagtg gtcggcggtg cagccggact gattgaggaa
    28981 gtggcagcca gcaaaatcag cggtgaagaa gatcgctaca gccacaccga tctgtgggat
    29041 ttccaggcta acgttgaagg ctcgcagaaa attgtcgatc tgctgcgtcc acaactgcaa
    29101 aaagccaacc cggaactgtt ggcaaaagtc gatgccaact ttaaaaaggt cgataccatt
    29161 ctggcgaaat accgtactaa agacggtttt gaaacctacg acaaattgac cgatgccgac
    29221 cggaatgcac tgaaaggacc gattactgcg ctggcggaag atctggcgca acttcgcggt
    29281 gtgctgggat tggattaagc attatgcagt atgaagataa aaacggcgtg aatgaaccgt
    29341 cacgccgacg tttactgaaa ggcattggtg cgctggcgct ggcgctggcg ggaagttgtc
    29401 cggtcgctca tgcacaaaaa acgcaaagtg cgccgggtac gctttcaccg gatgcacgca
    29461 atgagaaaca gccgttttat ggtgagcatc aggcagggat cctgacgcca caacaggccg
    29521 caatgatgct ggtggcgttt gatgtgcttg ccagcgataa acccgatctt gaacggttgt
    29581 ttcgcttgtt gactcagcgt tttgcttttc tgactcaggg cggagcggca ccagaaacgc
    29641 caaatccgcg cctgccacca ctcgattccg gcattcttgg cggctacatt gcgcccgata
    29701 atctcaccat cacgttatcg gtgggtcact cattgtttga tgagcgcttt ggccttgcgc
    29761 cacagatgcc gaaaaagttg cagaagatga cgcgttttcc caacgactcg ctggatgcgg
    29821 cgttatgtca tggtgatgtg ttgctacaaa tttgcgccaa cacccaggac acggtgatcc
    29881 atgcgctgcg cgatatcatc aaacatacgc cagatttgct cagcgtgcgc tggaagcggg
    29941 aagggtttat ttccgatcac gcggcgcgta gtaaagacaa agagacgccg attaatttgc
    30001 tgggtttcaa agacggcact gccaatcccg atagccagaa tgataagttg atgcaaaaag
    30061 tggtgtgggt aacggcagat cagcaggagc cagcgtggac aatcggtggc agctatcagg
    30121 cagtacgctt gattcagttt cgagtggaat tttgggacag aactccgctg aaagaacagc
    30181 agacgatttt tggtcgtgat aagcaaaccg gtgcgccgct gggaatgcag catgagcatg
    30241 atgttcccga ttacgccagc gatccggaag ggaaggtgat tgcgctggac agccatatcc
    30301 ggctggcgaa tccccgcacg gcggagagtg agtccagcct gatgctgcgt cgtggctaca
    30361 gttattcact gggcgtcacc aactccgggc aactggatat ggggctactg tttgtctgtt
    30421 accaacacga tctggaaaaa ggcttcctga ctgtacaaaa aaggctcaat ggcgaagcgc
    30481 tggaggaata cgttaaacct atcggcggcg gttatttttt tgcgctgccg ggagtaaagg
    30541 acgcgaacga ttatctcgga agcgcgttat tgcgggttta atgtttatag acggataagg
    30601 cgttcttgcg cagatgcctg atgcgatgct tgcgcgtctt cttatgccta caatcggtgc
    30661 gggttcggta ggcggataag gcgttcacgc cgcatccggc gttcgtgcgc agatgcctga
    30721 tgcgaatcct gctgaaagca cacagctttt ttcatatctg tcatcactct gtcatctttc
    30781 cagtagaaac taatgtcact gaaatggtgt tttatggtta aatataagtg aatatattgt
    30841 tgcaatgaat gcgagatctg ttgtaattat taagtagcag cggaagttcc cggcagtgat
    30901 agtcagtcac tatggagatc gcggatggta acgtcctgtt ctggacaatg tcttgttaag
    30961 ctacgctcca ccacttgcgg agatttctcc tccggtagca acttcgtcac tctgtatttt
    31021 tctcacttcc tctctttact cgtgttaccg acgccgcaaa cggtgtgcgt agccgttttt
    31081 cggcatttta tccaaaagca aactatggct tacaaggaag ccaaccctct gatgttcgtg
    31141 cgcataatcg cgctgccaac ggcgcgtgtg atgaatacaa acaactcaag gtgctctcca
    31201 tgggaagaca aaaagcagtg atcaaagctc gtcgtgaggc aaaacgtgtg ctgagacggg
    31261 attcgcgcag ccataagcag cgtgaagaag aatcggtcac ctcgcttgtg cagatgggcg
    31321 gcgtagaagc cattggtatg gcccgcgaca gtcgcgatac ttcgcccatc ctcgcgcgaa
    31381 atgaagcgca attgcactat ctgaaggcta ttgagagtaa gcagctgata ttcgccacgg
    31441 gcgaagccgg gtgcggaaaa acctggatca gcgcagcaaa agcggcagag gccctgatac
    31501 ataaggatgt cgacaggatt atcgtcaccc gtccagttct gcaagccgat gaagatcttg
    31561 gcttcttacc tggagatatc gcagaaaagt ttgctcccta ttttcgcccg gtctacgacg
    31621 tgctggtccg gcgcttaggg gcttccttta tgcagtactg cctgcgaccg gaaataggga
    31681 aggtggaaat tgcgccgttc gcctatatgc gtggacgtac ctttgaaaat gcagtcgtca
    31741 ttcttgacga ggcgcagaat gtgactgccg cgcaaatgaa aatgttttta acccgcctcg
    31801 gggagaacgt gacggttatc gtcaacggtg atatcacgca atgcgatttg cctcgcggcg
    31861 tgtgctccgg attaagtgac gcgctggaac gtttcgaaga agatgaaatg gttgggatcg
    31921 tccggttcgg taaagaggac tgcgtacgtt cggcactttg ccaacgtacg ctgcatgcat
    31981 acagttaagt gtattatcgg tgcagagccc gggcgaaccg ggctttgttt tgggtgttta
    32041 tgcccggact agcgcttttt ctgaaacaac catttttatt tgcccctggc tggtgaagtg
    32101 tacgctcatc ctgtggcttt tttgtagttg ctgatagagc tcatcaggta ttgctaagct
    32161 ctctgcatat tcttgcggcg tatattggta ggctgcatga tgctgctgtt tttgaaaacg
    32221 cagcttattg tacagcgccc agacaattaa cacgacggca ttcgccactg ccagcaaaaa
    32281 atagaactga agtcggcttc tggcctcgct ttgccagtaa taacccgtca gcagatccat
    32341 ggcgaataag aatatgaaca acgcaaataa tgtccacaag atggttgtgg caacataatc
    32401 aaccagtaaa cgtactggtg attgtcgggt cgtaataatt aaattgttca tattaacctc
    32461 tcagaatccc gcgatcggga cttacccaac gggcgcgttg ctttttaggc atcaacatga
    32521 ctcgtgtaaa tgataccaat gtcgttgtca ggctcagcat ccagaaaata accgggaacc
    32581 aaataatcca gaaaagcgat gaagtcagat tatgctcata gcgattctcg atcatcaggc
    32641 tgacaataaa ttgcagtaaa cataacgtac acaataatat tccggcagta tgtgtcgcag
    32701 cgatatgtgt caattcaata tttaacggta caccggcaag ttggactgcg taaataatga
    32761 aaccgaccag gcaggtgaag gcccatattg tcgtcaggca gtattcaaaa aacagcggcc
    32821 acattcgaaa gttttctttg cgccacaacc ttgtcatatt tttgaggaat acttctgcac
    32881 cgccctgagc ccagcgcagg cgctgtttcc acagcccttt taacgtttca ggcattaata
    32941 tccagcacag cgcccgtggc tcgtaaaaaa tcgtccactg attcaactgc agcttccagc
    33001 taatatcaat atcttcggtg atcatatcgt cactccagta acccacttct gccagggcgc
    33061 tgcgacgaaa tgcggcaata acaccggaaa cggtaaatac gtttccatag atacgctggg
    33121 ttcgcttgat caaaccaata attgaggaat actcgccaac ctgaatttta cccaccaggg
    33181 tagaacgtgt tcgaatacga ggattaccgg ttacggcacc cacacgcggg ttgtacaaca
    33241 tcggttccac aatatatgcc gccgcatcgc ggtctaataa cgcatcgcca tcaatgcaca
    33301 ccagatattc acttttcgcc gcggcagctc cggttttaag cgcaatggct ttcccctggt
    33361 tttgcgccag atgaatgacc cgcaaatggg gaatttgtgc agccatgcga tccaggatgg
    33421 cacgggtttt atctgttgaa ccgtcattta cggcaataac ttcaatgttc tcataacgct
    33481 gtgctaaagc ggcgtgtatg gtttcctcaa cgtttttctc ctcattaaaa cagggaatga
    33541 taatggagat agacggatta tctttcaact ggggagctgg tgcgttttct ccccacggcc
    33601 agtggcgttc acgatagacc cagaaataga cgccgccaac aatccacata atggacataa
    33661 aaaacggcca gaagaaaacg aacctcatca tcagttcacc agagtgaaag tacgctacgc
    33721 ataggggtat gcataacacc agacataata taaaaaacga tacgatgcga ttaatcattt
    33781 ttcggatacc aggctgttga aaactcagga cgaataaggt ctatttcagg ttggttatgc
    33841 agaaaattgt cgggataata accatagttt ttcactccat tcagttgtaa taggctcatc
    33901 cagtgagcga gttgttgcga agaaatagcc tgatgctgac catttttctg ccagttttgt
    33961 gcctgtaatt ctaaaataga tttgtcttta gcctgaggga tgtttttaat ttgattggtc
    34021 aattgtatta accattggtc agccgatttt tctgcgacac cttccagata aggcatagcc
    34081 ataatagcgg tccagtcata gctttttagg aaatcagcat aattctgtgc aaaccaggct
    34141 tcactttcag gttgtattac cggaagtgca aaaatatttc gtgcagtttt aatatgtgga
    34201 ccgcgaatgg cttttacgcg cgcactaagt tctaaagtga agtcagttaa cgcacgactt
    34261 ttaaagcggg cccactgttt aaattgctcc gggttttgtc gaatttcgct cagactcccg
    34321 ctaaagcctg cttgctgata agccgtgata gccggtgcac tggcatcttc ataatctgaa
    34381 agcaaagcat catcgtggaa caatatgcca tcaaaagcag catgtccggc aagatcttca
    34441 tataacatgc caacttgtgc tctgactctg tcatcgaaag gagagagacg gtgatattgt
    34501 tcaggatgaa tttgtgcttt tttctcccct gttggtaagt attttactcg cgttaatgtg
    34561 ggatctaaat cccagcttaa taccggcatc cacgcataga tgtttacacc tgagcgggta
    34621 cgtaattgcc aggcaacccg actaaaaata tctgctttca ttggtagcaa acgatttgga
    34681 aaccagacct ctttgaccag cccatcacca tcgggatcag caaatgcctg caaatacacg
    34741 gttgatattt gcatatcttt cacccgctga attagcacat caatattgcg atccatttgc
    34801 tggaggtttt cgtcataaac gtaatcaaga tcgatatgca ttatccgttg tggtgatttt
    34861 tcctgtacgg taataatttg ctgggcaaac tcttttaatg agggattatt ggcgattaat
    34921 acccgcggaa tggaatccaa ttgcgacgca tttgccaaac ctgattcaag ggtgaagaac
    34981 atgtcataac cgagtttttt taattcctct atcgctatgc cattcgcttc gccataaggc
    35041 caaacaaaaa cgtgtggatt tacctcaacc tttgtacgca ggtattccgt catttttaca
    35101 gcatccagac gaattctttc ccggtattct gctgcggttt cataccgtgc gtggtcagta
    35161 aaatatgcac gatttacata tacaggcaat aagctgccgg tggcattagc ctgaataccg
    35221 tagtgagaat tccatgtatg agaagcgagc tcaacgagcc gggaacgcgc aacttctcgc
    35281 acttgttgcc acgtggcaaa atattctcga tcgaccaact catcgccaaa ttttacttgt
    35341 ttatccgctg gcgtatcgac ccaactgccg acgggggccc atacagcagg ccactggaag
    35401 gcctgaagaa ttgggaagac gcgggtataa aaactctggt agccgtcatc aaaagtcagc
    35461 actacagctt tttccggtag cggttttcct cctcgatgtg cttcacgaat ttgagcaata
    35521 ctgaccggtt gataaccgtt ctcgcgcagc caggcaaatt gttcacgcag tgctgatgtc
    35581 cgcactgaca taaaacgctg gtcggcagct tcgtcttcaa cgttatgcca tgaaatcgct
    35641 acaaaaccat tatgcggcca cggttgctcg gcgagtaaag attcgcgatc ctgtggcggt
    35701 ataaatgatg ttcttgactg gctaatgcac gcggtgagca taattatact caccagcatc
    35761 aggagatatt tatttccatt acgtaacata tttatcctta aaatctgaat gtcatatcga
    35821 attcaacgta taagttgtgt tctctgtcac cgtcataagg tcgtttttcc cagcgtagcg
    35881 ttgcgccagc atcaatcacg tcattccagc taatgcgttg cccgtagccg agttgggtga
    35941 cgacatccgt gccataatgt ttttgccagg aggcaccaac acctgcgctg aatatttgct
    36001 cccagctatt ttcatagctt cgccataaca aatggcttgc ctcaaatgcc ggaacaatat
    36061 cgaacgtttt tatagggttg tagtatgggg tatcgtgttc tgtattttgt tcgtaataca
    36121 gactgggtag gaaatcgaca atcaaatatg gtgaagacca gatgcgttcc tgaccctcaa
    36181 gtgagacttc atgacgctgg ttactgtcgg aaaaatcagt gaaagcccag gagacaccgt
    36241 acttacgccg ctcattttga taccagcgaa cataagcctg agcactgttg cctgtaacac
    36301 catttttcat tgcccgtaat ggaacgcggt gagagaggcg ttccagttgc gaaccaatac
    36361 gccagttatc attaaaatca taccagccag acaggcgcgc gccgggttta tgctcatgat
    36421 tgaaaacgcg ttcagcgtac tctgcctcga gccagatatt acgtgaccgc cactcaacac
    36481 ccgcaagcca gtcgcgaaca atcccttttc cttcgctaaa ttgtccatcg gcataaccga
    36541 atccagcaaa accgcgccag ttatccttca gcggtggtga ataaacgatg gtggttaagt
    36601 cgacatcatg tttaccacta tccgggcctt cggcatcaat tcctgttgag ccagcgatac
    36661 gaagctctgc aagattatgt acatcaaccg cacgttttaa tcgtacaacg ccgggatctt
    36721 gcggttcacg ttcgacaaca tcgtgcgtta agacagctgc ctgctgccat tcttgtaacg
    36781 ttaatgctgt ccaggcttgt tcaacctcca gattaatatt acgtggctcg atcacttctg
    36841 ctttttttaa ttcattttct gctgcacgag gccaaccgcg ggcttgtaac acactcgcgt
    36901 aatcaatgcg cagtccctga tttcctggtg cgttataagc aagctctctg gctgtcattt
    36961 cagcctgagg aagatcatta ctatattttg ctacggttga gagaaacgaa tgcccctgta
    37021 accaggtatc attcgggatg ctcgtaggcg tgcccattaa ccgaaggaaa ggcggcgaag
    37081 tattaatggt atgttgggtg acagttagcg cgcccggata attttcactc tccaggtggc
    37141 tgtaaaagag atccgcaagt tcttcatcgg ataaatccgg ggcaatggtc tccttgtgat
    37201 aaaagagctc ggtcattatt gactgtgctt ttttcggctg atgatctttg agataagccg
    37261 atgcaaccca atattgcccc cagggcggaa taatttgccc cgtctttttt aatcgctgat
    37321 agtgagaaat aacgtcttta taacgatcgc gagttaataa cgcgccaaga tgatcaacct
    37381 gaatacgctg gtactgggca gtacggtctg ggttatcgtg ccacagaatt tctaatgcag
    37441 cgtattgggc gagggcgcga tcggcaatgg cataacgttc actttcactg cgcgtaggca
    37501 taaacgacag tctgaccagt tcggcatgaa tatcagcgcg aatatctggc gttaaattgg
    37561 catcgtcaat cgcggcagca agttgattat tacgtaatgc ctgcacgtat tctgtgggat
    37621 attgttgcgt agatgcattt tcaggtaatg actctgtcat cgcccgtaat tcatcctgat
    37681 gacgccccgc cagtttatag atataggctt ctgcgagtaa attggctttg tccggtgctc
    37741 cagagttaag ctgcttaagt ttaaccagcg cagtatcata gtgaccagca tctgccaggg
    37801 ttaaaatttg tccccgttga taatccttat tttgcggctc cagagagagc gccttttgcc
    37861 acagtgtaag cgagttttgc cattgttgca ggttacgata agcgacggcg acagctgcat
    37921 aaccacgcgc tggtaattgc tgatgacggt agcggttgta aacggtaata acctgtttat
    37981 cttgcccggc ccataaggca atctgtaacc agtcagcaat ttggttattg ctgagtgctg
    38041 attttagtgc aaaccatgac aaagctggct gagtattacc cttgcgagct tcaataatca
    38101 atgcatcata ggcgttatta acagcactct tcgccgcggg actcgctgct aaaaatgcgg
    38161 cagtaagaag tttcaaagcc catttggttt tcgggcacct ttttctgcta cttgaataca
    38221 tcctgtatta ctccatgtat tgccaaaatc tctctctgta tctaattaca ggtaactgaa
    38281 aagaaagata tttttgcacc tcataatccg ttattaaacg cggaagagag acgtgaattg
    38341 ttgatgatga gaagaagaaa tgatgagcag agtgtccata taaaatcctt ttctcgcccg
    38401 aaaa
//
LOCUS       DAZFQD010000032        38178 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.032, whole genome shotgun sequence.
ACCESSION   DAZFQD010000032 DAZFQD010000000
VERSION     DAZFQD010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 38178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38178
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.032"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            138..542
                     /gene="ybgC"
                     /locus_tag="ACHKTW_001711"
     CDS             138..542
                     /gene="ybgC"
                     /locus_tag="ACHKTW_001711"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HFO6462757.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            539..1231
                     /gene="tolQ"
                     /locus_tag="ACHKTW_001712"
     CDS             539..1231
                     /gene="tolQ"
                     /locus_tag="ACHKTW_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HFO6462758.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGVSRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            1235..1663
                     /gene="tolR"
                     /locus_tag="ACHKTW_001713"
     CDS             1235..1663
                     /gene="tolR"
                     /locus_tag="ACHKTW_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HFO6462759.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            1728..2993
                     /gene="tolA"
                     /locus_tag="ACHKTW_001714"
     CDS             1728..2993
                     /gene="tolA"
                     /locus_tag="ACHKTW_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0019534 - toxin transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HFO6462760.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEEAAAKAAADAKAKAEADAKAAEEAA
                     KKAAADAKKKAEAEAAKAAAEAQKKAEAAAAALKKKAEAAEAAAAEARKKAATEAAEK
                     AKAEAEKKAAAEKAAADKKAAAEKAAADKKAAEKAAAEKAAADKKAAAEKAAADKKAA
                     AAKAAAEKAAAAKAAAEADDIFGELSSGKNAPKTGGGAKGNNASPAGSGNTKNNGASG
                     ADINNYAGQIKSAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAAL
                     AAAKLAKIPKPPSQAVYEVFKNAPLDFKP"
     gene            3126..4418
                     /gene="tolB"
                     /locus_tag="ACHKTW_001715"
     CDS             3126..4418
                     /gene="tolB"
                     /locus_tag="ACHKTW_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HFO6462761.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            4453..4974
                     /gene="pal"
                     /locus_tag="ACHKTW_001716"
     CDS             4453..4974
                     /gene="pal"
                     /locus_tag="ACHKTW_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HFO6462762.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMY
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            4984..5775
                     /gene="cpoB"
                     /locus_tag="ACHKTW_001717"
     CDS             4984..5775
                     /gene="cpoB"
                     /locus_tag="ACHKTW_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0070206 - protein trimerization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HFO6462763.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVGSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSSDQSGAAASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            5940..6015
                     /locus_tag="ACHKTW_001718"
     tRNA            5940..6015
                     /locus_tag="ACHKTW_001718"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5973..5975,aa:Lys,seq:ttt)
     gene            6151..6226
                     /locus_tag="ACHKTW_001719"
     tRNA            6151..6226
                     /locus_tag="ACHKTW_001719"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6184..6186,aa:Val,seq:tac)
     gene            6229..6304
                     /locus_tag="ACHKTW_001720"
     tRNA            6229..6304
                     /locus_tag="ACHKTW_001720"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6262..6264,aa:Lys,seq:ttt)
     gene            6454..6529
                     /locus_tag="ACHKTW_001721"
     tRNA            6454..6529
                     /locus_tag="ACHKTW_001721"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6487..6489,aa:Val,seq:tac)
     gene            6533..6608
                     /locus_tag="ACHKTW_001722"
     tRNA            6533..6608
                     /locus_tag="ACHKTW_001722"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6566..6568,aa:Lys,seq:ttt)
     gene            6642..6717
                     /locus_tag="ACHKTW_001723"
     tRNA            6642..6717
                     /locus_tag="ACHKTW_001723"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6675..6677,aa:Lys,seq:ttt)
     gene            6748..6873
                     /locus_tag="ACHKTW_001724"
     ncRNA           6748..6873
                     /ncRNA_class="other"
                     /locus_tag="ACHKTW_001724"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            7122..8165
                     /gene="nadA"
                     /locus_tag="ACHKTW_001725"
     CDS             7122..8165
                     /gene="nadA"
                     /locus_tag="ACHKTW_001725"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HFO6462764.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQAECSLDLGCPVEEFNAFCDAHPDRTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKQTGGDILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKTLPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNGLQSIAEALEQEGSNHEVHVDERLRERALVP
                     LNRMLDFAATLRG"
     gene            8203..8922
                     /gene="pnuC"
                     /locus_tag="ACHKTW_001726"
     CDS             8203..8922
                     /gene="pnuC"
                     /locus_tag="ACHKTW_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HFO6462765.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(8919..9860)
                     /gene="zitB"
                     /locus_tag="ACHKTW_001727"
     CDS             complement(8919..9860)
                     /gene="zitB"
                     /locus_tag="ACHKTW_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HFO6462766.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVVGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHATIQMEYQPCHGPDCHLNEGVSGHSHHHH"
     gene            complement(9974..10354)
                     /gene="ybgS"
                     /locus_tag="ACHKTW_001728"
     CDS             complement(9974..10354)
                     /gene="ybgS"
                     /locus_tag="ACHKTW_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HFO6462767.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHSDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            10670..11722
                     /gene="aroG"
                     /locus_tag="ACHKTW_001729"
     CDS             10670..11722
                     /gene="aroG"
                     /locus_tag="ACHKTW_001729"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HFO6462768.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLESGEPLAYGKSITDACIGWEDT
                     DALLRQLANAVKARRG"
     gene            complement(11880..12632)
                     /gene="gpmA"
                     /locus_tag="ACHKTW_001730"
     CDS             complement(11880..12632)
                     /gene="gpmA"
                     /locus_tag="ACHKTW_001730"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFO6462769.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(12837..13877)
                     /gene="galM"
                     /locus_tag="ACHKTW_001731"
     CDS             complement(12837..13877)
                     /gene="galM"
                     /locus_tag="ACHKTW_001731"
                     /EC_number="5.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004034 - aldose 1-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HFO6462770.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILADEYLPVDEGGIPHDVLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAE"
     gene            complement(13871..15019)
                     /gene="galK"
                     /locus_tag="ACHKTW_001732"
     CDS             complement(13871..15019)
                     /gene="galK"
                     /locus_tag="ACHKTW_001732"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HFO6462771.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLDAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            complement(15023..16069)
                     /gene="galT"
                     /locus_tag="ACHKTW_001733"
     CDS             complement(15023..16069)
                     /gene="galT"
                     /locus_tag="ACHKTW_001733"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HFO6462772.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESHDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAEREERLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            complement(16079..17095)
                     /gene="galE"
                     /locus_tag="ACHKTW_001734"
     CDS             complement(16079..17095)
                     /gene="galE"
                     /locus_tag="ACHKTW_001734"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308814.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFO6462773.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVGESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPKIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            complement(17356..18828)
                     /gene="modF"
                     /locus_tag="ACHKTW_001735"
     CDS             complement(17356..18828)
                     /gene="modF"
                     /locus_tag="ACHKTW_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HFO6462774.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPGEDDTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKSLLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDEIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANEPRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLVTGDHPQGYSNDLTLFGRRRGSGETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQAVSDRQQKLVQQWLDILGIDK
                     RTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYRYVLTKIY"
     gene            complement(18896..19684)
                     /gene="modE"
                     /locus_tag="ACHKTW_001736"
     CDS             complement(18896..19684)
                     /gene="modE"
                     /locus_tag="ACHKTW_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HFO6462775.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            19813..19962
                     /gene="acrZ"
                     /locus_tag="ACHKTW_001737"
     CDS             19813..19962
                     /gene="acrZ"
                     /locus_tag="ACHKTW_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HFO6462776.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            20129..20902
                     /gene="modA"
                     /locus_tag="ACHKTW_001738"
     CDS             20129..20902
                     /gene="modA"
                     /locus_tag="ACHKTW_001738"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO6462777.1"
                     /translation="MARKWLNLFAGAALSFAVAGNTLADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTATRQ
                     TLLGNSLVVVAPKASEQKDFTIDSKTNWTSLLSGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVASKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            20902..21591
                     /gene="modB"
                     /locus_tag="ACHKTW_001739"
     CDS             20902..21591
                     /gene="modB"
                     /locus_tag="ACHKTW_001739"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HFO6462778.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITIPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPGETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            21594..22652
                     /gene="modC"
                     /locus_tag="ACHKTW_001740"
     CDS             21594..22652
                     /gene="modC"
                     /locus_tag="ACHKTW_001740"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HFO6462779.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMA
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLDEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELV
                     IKPGLWLYAQIKSVSITA"
     gene            complement(22653..23471)
                     /gene="ybhA"
                     /locus_tag="ACHKTW_001741"
     CDS             complement(22653..23471)
                     /gene="ybhA"
                     /locus_tag="ACHKTW_001741"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyridoxal
                     phosphate/fructose-1,6-bisphosphate phosphatase"
                     /protein_id="HFO6462780.1"
                     /translation="MTTRVIALDLDGTLLTPKKTLLPSSIEALARAREAGYQLIIVTG
                     RHHVAIHPFYQALALDTPAICCNGTYLYDYHAKTVLEADPMPVNKALQLIEMLNEHHI
                     HGLMYVDDAMVYEHPTGHVIRTSNWAQTLPPEQRPTFTQVASLAETAQQVNAVWKFAL
                     THDDLPQLQHFGKHVEHELGLECEWSWHDQVDIARGGNSKGKRLTKWVEAQGWSMENV
                     VAFGDNFNDISMLEAAGTGVAMGNADDAVKARANIVIGDNTTDSIAQFIYSHLI"
     gene            23626..24621
                     /gene="pgl"
                     /locus_tag="ACHKTW_001742"
     CDS             23626..24621
                     /gene="pgl"
                     /locus_tag="ACHKTW_001742"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HFO6462781.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESALPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDHSGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            complement(24662..25615)
                     /gene="ybhD"
                     /locus_tag="ACHKTW_001743"
     CDS             complement(24662..25615)
                     /gene="ybhD"
                     /locus_tag="ACHKTW_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308823.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO6462782.1"
                     /translation="MKHELSSMKAFVILAESSSFNNAAKLLNITQPALTRRIKKMEED
                     LHIQLFERTTRKVTLTKAGKRLLPEARELIKKFDETLFNIRDMNAYHRGMVTLACIPT
                     AVFYFLPLAIGKFNELYPNIKVRILEQGTNNCMESVLCNESDFGINMNNVTNSSIDFT
                     PLVNEPFVLACRRDHPLAKKQLVEWQELVGYKMIGVRSSSGNRLLIEQQLADKPWKLD
                     WFYEVRHLSTSLGLVEAGLGISALPGLAMPHAPYSSIIGIPLVEPVIRRTLGIIRRKD
                     AVLSPAAERFFALLINLWTDDKDNLWTNIVERQRHALQEIG"
     gene            25799..26851
                     /gene="ybhH"
                     /locus_tag="ACHKTW_001744"
     CDS             25799..26851
                     /gene="ybhH"
                     /locus_tag="ACHKTW_001744"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HFO6462783.1"
                     /translation="MKKIPCVMMRGGTSRGAFLLAEHLPEDQTQRDKILMAIMGSGND
                     LEIDGIGGGNPLTSKVAIISRSSDPRADVDYLFAQVIVHEQRVDTTPNCGNMLSGVGA
                     FAIENGLIAATSPVTRVRIRNVNTGTFIEADVQTPNGVVEYEGSARIDGVPGTAAPVA
                     LTFLNAAGTKTGKVFPTDNQIDYFDDVPVTCIDMAMPVVIIPAEYLGKTGYELPAELD
                     ADKALLARIESIRLQAGKAMGLGDVSNMVIPKPVLISPAQKGGAINVRYFMPHSCHRA
                     LAITGAIAISSSCALEGTVTRQIVPSVGYGNINIEHPSGALDVHLSNEGQDATTLRAS
                     VIRTTRKIFSGEVYLP"
     gene            26927..28360
                     /gene="ybhI"
                     /locus_tag="ACHKTW_001745"
     CDS             26927..28360
                     /gene="ybhI"
                     /locus_tag="ACHKTW_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HFO6462784.1"
                     /translation="MNKKSLWKLILILAIPCIIGFMPAPAGLSELAWVLFGIYLAAIV
                     GLVIKPFPEPVVLLIAVAASMVVVGNLSDGAFKTTAVLSGYSSGTTWLVFSAFTLSAA
                     FVTTGLGKRIAYLLIGKIGNTTLGLGYVTVFLDLVLAPATPSNTARAGGIVLPIINSV
                     AVALGSEPEKSPRRVGHYLMMSIYMVTKTTSYMFFTAMAGNILALKMINDILHLQISW
                     GGWALAAGLPGIIMLLVTPLVIYTMYPPEIKKVDNKTIAKAGLAELGPMKIREKMLLG
                     VFVLALLGWIFSKSLGVDESTVAIVVMATMLLLGIVTWEDVVKNKGGWNTLIWYGGII
                     GLSSLLSKVKFFEWLAEVFKNNLAFDGHGNVAFFVIIFLSIIVRYFFASGSAYIVAML
                     PVFAMLANVSGAPLMLTALALLFSNSYGGMVTHYGGAAGPVIFGVGYNDIKSWWLVGA
                     VLTILTFLVHITLGVWWWNMLIGWNML"
     gene            28543..30804
                     /gene="ybhJ"
                     /locus_tag="ACHKTW_001746"
     CDS             28543..30804
                     /gene="ybhJ"
                     /locus_tag="ACHKTW_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HFO6462785.1"
                     /translation="MIKLSEKGVFLASNNEIIAEEHFTGEIKKEEAKKGTIAWSILSS
                     HNTSGNMDKLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGT
                     INGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMAGGGKMILGSDSHTRYGALGTM
                     AVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKPAPYVGPQDVALAIIGAVFKNGYVK
                     NKVMEFVGPGVSALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQL
                     NPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYKIDTLNQNLTDILREIEIESERVAH
                     GKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPS
                     SQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNREG
                     SKPANGQMSAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQG
                     FVKGATQQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRS
                     NPIGLAEFTLSRRDPGYVGRSKATAELENQRLAGNVSELTEVFARIKQIAGQEHIDPL
                     QTEIGSMVYAVKPGDGSAREQAASCQRVIGGLANIAEEYATKRYRSNVINWGMLPLQM
                     AEVPTFEVGDYIYIPGIKAALDNPGTTFKGYVIHEDAPVTEITLYMESLTAEEREIIK
                     AGSLINFNKNRQM"
     gene            complement(31038..32321)
                     /gene="ybhC"
                     /locus_tag="ACHKTW_001747"
     CDS             complement(31038..32321)
                     /gene="ybhC"
                     /locus_tag="ACHKTW_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HFO6462786.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEAQNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     AFGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(32473..32949)
                     /gene="ybhB"
                     /locus_tag="ACHKTW_001748"
     CDS             complement(32473..32949)
                     /gene="ybhB"
                     /locus_tag="ACHKTW_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HFO6462787.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDVERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            complement(33008..34297)
                     /gene="bioA"
                     /locus_tag="ACHKTW_001749"
     CDS             complement(33008..34297)
                     /gene="bioA"
                     /locus_tag="ACHKTW_001749"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HFO6462788.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVASAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKSQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEHAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAILESGDWQQ
                     QVAAIEVQLREQLAPARDAEMVADVRVLGAIGVVETTHPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            34384..35424
                     /gene="bioB"
                     /locus_tag="ACHKTW_001750"
     CDS             34384..35424
                     /gene="bioB"
                     /locus_tag="ACHKTW_001750"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HFO6462789.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFDPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLEQALMT
                     PDTDEYYNAAAL"
     gene            35421..36575
                     /gene="bioF"
                     /locus_tag="ACHKTW_001751"
     CDS             35421..36575
                     /gene="bioF"
                     /locus_tag="ACHKTW_001751"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HFO6462790.1"
                     /translation="MSWQEKINAALDARRAADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWQQGAEQFGVGSGGSGHVSGYSVAHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSQLRRFAHNDVTHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRRDEGDARREKLVSLIARFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            36562..37317
                     /gene="bioC"
                     /locus_tag="ACHKTW_001752"
     CDS             36562..37317
                     /gene="bioC"
                     /locus_tag="ACHKTW_001752"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010340 - carboxyl-O-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HFO6462791.1"
                     /translation="MATVNKQAIAAAFGRAAALYEQHADLQRQSADVLLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLVQARQKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQAWQAVDERP
                     HANRFLPPDEIEQSLNGVHYQHHIQPITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            37310..37987
                     /gene="bioD"
                     /locus_tag="ACHKTW_001753"
     CDS             37310..37987
                     /gene="bioD"
                     /locus_tag="ACHKTW_001753"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFO6462792.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAVGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQHNSSLQLDYATVNPYTFAEPTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALL"
BASE COUNT         9262 a         9475 c        10183 g         9258 t
ORIGIN      
        1 tttaaaaaat tatgggccgc tccaggccca taaattttta cgctccctta acttgccctc
       61 attcccaaac ctcaatcgcg cgcgtatagt agcagcgttt aaaagctcta acttttgttg
      121 cattaccggg atgtaaagtg aatacaacgc tgtttcgatg gccggttcgc gtctactatg
      181 aagataccga tgccggtggt gtggtgtacc acgccagtta cgtcgctttt tatgaaagag
      241 cacgcacaga gatgctgcgt catcatcact tcagtcagca ggcgctgatg gctgaacgcg
      301 ttgcctttgt ggtacgtaaa atgacggtgg aatattacgc acctgcgcgg ctcgacgata
      361 tgctcgaaat acagactgaa ataacatcaa tgcgtggcac ctctttggtt ttcacgcaac
      421 gtattgtcaa cgccgagaat actttgctga atgaagcaga ggttctggtt gtttgcgttg
      481 acccactcaa aatgaagcct cgtgcgcttc ccaagtctat tgtcgcggag tttaagcagt
      541 gactgacatg aatatccttg atttgttcct gaaggctagc cttctggtta aacttatcat
      601 gttgattttg attggttttt caatcgcatc ttgggccatt attatccagc ggacccgtat
      661 tcttaacgca gcggcgcgcg aagccgaagc gtttgaagat aaattctggt ctggaatcga
      721 actctctcgc ctctatcaag agagccaggg gaaacgggat aatctgactg gttcggaaca
      781 aatcttttac agcgggttca aagagtttgt gcgcctgcat cgtgccaata gccatgcgcc
      841 ggaagccgta gtggaagggg tgtcgcgtgc tatgcgtatc tccatgaacc gtgaacttga
      901 aaatctggaa acgcacattc cgttcctcgg tacggttggc tccatcagcc cgtatattgg
      961 tctgtttggt acggtctggg ggatcatgca cgcctttatc gccctcgggg cggtaaaaca
     1021 agcaacactg caaatggttg cgcccggtat cgcagaagcg ttgattgcga ctgcaattgg
     1081 tctgttcgcc gcaattccgg cggtaatggc ttataaccgc ctcaaccagc gcgtaaacaa
     1141 actggaactg aattacgaca actttatgga agagtttacc gcgattctgc accgccaggc
     1201 gtttaccgtt agcgagagca acaaggggta agccatggcc agagcgcgtg gacgaggtcg
     1261 tcgcgatctc aagtccgaaa tcaacattgt accgttgctg gacgtactgc tggtgctgtt
     1321 gctgatcttt atggcgacag cgcccatcat cacccagagc gtggaggtcg atctgccaga
     1381 cgctactgaa tcacaggcgg tgagcagtaa cgataatccg ccagtgattg ttgaagtgtc
     1441 tggtattggt cagtacaccg tggtggttga gaaagatcgc ctggagcgtt taccaccaga
     1501 gcaggtggtg gcggaagtgt ccagccgttt caaggccaac ccgaaaacgg tctttctgat
     1561 cggtggcgca aaagatgtgc cttacgatga aataattaaa gcactgaact tgttacatag
     1621 tgcgggtgtg aaatcggttg gtttaatgac gcagcctatc taaacatctg cgtttccctt
     1681 gcttgaaaga gagcgggtaa caggcgaaca gtttttggaa accgagagtg tcaaaggcaa
     1741 ccgaacaaaa cgacaagctc aagcgggcga taattatttc agcagtgctg catgtcatct
     1801 tatttgcggc gctgatctgg agttcgttcg atgagaatat agaagcttca gccggaggcg
     1861 gcggtggttc gtccatcgac gctgtcatgg ttgattcagg tgcggtagtt gagcagtaca
     1921 aacgcatgca aagccaggaa tcaagcgcga agcgttctga tgaacagcgc aagatgaagg
     1981 aacagcaggc tgctgaagaa ctgcgtgaga aacaagcggc tgaacaggaa cgcctgaagc
     2041 aacttgagaa agagcggtta gcggctcagg agcagaaaaa gcaggctgaa gaagccgcaa
     2101 aacaggccga gttaaagcag aagcaagctg aagaggcggc agcgaaagcg gcggcagatg
     2161 ctaaagcgaa ggccgaagca gatgctaaag ctgcggaaga agcagcgaag aaagcggctg
     2221 cagacgcaaa gaaaaaagca gaagcagaag ccgccaaagc cgcagccgaa gcgcagaaaa
     2281 aagccgaggc agccgctgcg gcactgaaga agaaagcgga agcggcagaa gcagctgcag
     2341 ctgaagcaag aaagaaagcg gcaactgaag ctgctgaaaa agccaaagca gaagctgaga
     2401 agaaagcggc tgctgaaaag gctgcagctg ataagaaagc ggcagcagag aaagctgcag
     2461 ccgacaaaaa agcagcagaa aaagcggctg ctgaaaaggc agcagctgat aagaaagcag
     2521 cggcagaaaa agccgccgca gacaaaaaag cggcagcggc aaaagctgca gctgaaaaag
     2581 ccgctgcagc aaaagcggcc gcagaggcag atgatatttt cggtgagcta agctctggta
     2641 agaatgcacc gaaaacgggg ggaggggcga aagggaacaa tgcttcgcct gccgggagtg
     2701 gtaatactaa aaacaatggc gcatcagggg ccgatatcaa taactatgcc gggcagatta
     2761 aatctgctat cgaaagtaag ttctatgacg catcgtccta tgcaggcaaa acctgtacgc
     2821 tgcgcataaa actggcaccc gatggtatgt tactggatat caaacctgaa ggtggcgatc
     2881 ccgcactttg tcaggctgcg ttggcagcag ctaaacttgc gaagatcccg aaaccaccaa
     2941 gccaggcagt atatgaagtg ttcaaaaacg cgccattgga cttcaaaccg taatcgcgat
     3001 gttgactgtt cggacggtca acatcaggca ccggttgcca cggggttctg gtagttttgt
     3061 gtattttagt ttgttaacat tctgctaaat tatcgtgggc catcggtcca gataagggag
     3121 atatgatgaa gcaggcatta cgagtagcat ttggttttct catactgtgg gcatcagttc
     3181 tgcatgctga agtccgcatt gtgatcgaca gcggtgtaga ttccggtcgt cctattggtg
     3241 ttgttccttt ccagtgggcg gggcctggtg cggcacctga agatattggc ggcatcgttg
     3301 ctgctgactt gcgtaacagc ggtaaattta atccgttaga tcgcgctcgt ctgccacagc
     3361 agccgggtag tgcgcaggaa gtacaaccag ctgcatggtc cgcactgggc attgacgctg
     3421 tagttgtcgg tcaggtcact ccgaatccgg atggttctta caatgttgct tatcaacttg
     3481 ttgacactgg cggcgcaccg ggtactgtac ttgctcagaa ctcgtacaaa gtgaacaagc
     3541 agtggctgcg ttatgctggt cataccgcca gtgatgaagt gtttgaaaaa ctgaccggca
     3601 ttaaaggtgc gttccgtact cgtattgcct acgttgttca gaccaacggc ggtcagttcc
     3661 cgtatgaact gcgcgtatct gactatgacg gttacaacca gtttgtcgtt caccgttcac
     3721 cgcagccgct gatgtctccg gcgtggtcac cagacggttc taaactggct tatgtgacct
     3781 tcgaaagcgg tcgttccgcg ctggttattc agacgctggc aaatggcgct gtacgtcagg
     3841 tggcttcatt cccgcgtcac aacggtgcac ctgcattctc gccagacggc agcaaactgg
     3901 cattcgcctt gtcgaaaacc ggtagtctga acctgtacgt aatggatttg gcttctggtc
     3961 agatccgcca ggtgactgat ggtcgcagta acaataccga accgacctgg ttcccggata
     4021 gccagaacct ggcatttact tctgaccagg ccggtcgtcc gcaggtttat aaagtgaata
     4081 tcaacggcgg tgcgccacaa cgtattacct gggaaggttc gcagaaccag gatgcggatg
     4141 tcagcagcga cggtaaattt atggtaatgg tcagctccaa tggtgggcag cagcacattg
     4201 ccaaacaaga tctggcaacg ggaggcgtac aagttctgtc gtccacgttc ctggatgaaa
     4261 cgccaagtct ggcacctaac ggcactatgg taatctacag ctcttctcag gggatgggat
     4321 ccgtgctgaa tttggtttct acagatgggc gtttcaaagc gcgtcttccg gcaactgatg
     4381 gacaggtcaa attccctgcc tggtcgccgt atctgtgata ataattaatt gaatagtaaa
     4441 ggaatcattg aaatgcaact gaacaaagtg ctgaaagggc tgatgattgc tctgcctgtt
     4501 atggcaattg cggcatgttc ttccaacaag aacgccagca atgacggcag cgaaggcatg
     4561 ctgggtgccg gcactggtat gtatgcgaac ggcggcaacg gcaacatgtc ttccgaagag
     4621 caggctcgtc tgcaaatgca acagctgcag cagaacaaca tcgtttactt cgatctggac
     4681 aagtacgata tccgttctga cttcgctcaa atgctggatg cacatgcaaa cttcctgcgt
     4741 agcaacccgt cttacaaagt caccgtagaa ggtcacgcgg acgaacgtgg tactccggaa
     4801 tacaacatct ccctgggtga acgtcgtgcg aacgccgtta agatgtacct gcagggtaaa
     4861 ggcgtttctg cagaccagat ctccatcgtt tcttacggta aagaaaaacc tgcagtactg
     4921 ggtcatgacg aagcggcata ctccaaaaac cgtcgtgcgg tactggttta ctaagagaat
     4981 tgcatgagca gtaacttcag acatcaacta ttgagtctgt cgttactggt tggtatagcg
     5041 gccccctggg ccgcttttgc tcaggcacca atcagtagtg tcggctcagg ctcggtcgaa
     5101 gaccgcgtca ctcaacttga gcgtatttct aatgctcaca gccagctttt aacccaactc
     5161 cagcaacaac tttctgataa tcaatccgat attgattccc tgcgtggtca gattcaggaa
     5221 aatcagtatc aactgaatca ggtcgtggag cggcaaaagc agatcctgtt gcagatcgac
     5281 agcctcagca gcggtggtgc agcggcgcaa tcaaccagca gcgatcaaag cggtgcggcg
     5341 gcatcaacga cgccgacagc tgatgctggt actgcgaatg ctggcgcgcc ggtgaaaagc
     5401 ggcgatgcaa acacggatta caatgcagct attgcgctgg tgcaggataa atcccgccag
     5461 gatgacgcaa tggtggcatt tcagaatttc atcaaaaatt accctgattc aacttacctg
     5521 ccaaacgcca attattggct gggtcagtta aactacaaca agggtaaaaa agatgatgcg
     5581 gcgtactatt ttgcttcggt agtgaaaaac tatccgaagt caccaaaggc tgctgatgcg
     5641 atgtttaaag tcggcgtcat catgcaggac aaaggtgaca ccgcaaaagc gaaagccgta
     5701 taccagcagg ttatcagtaa ataccctggt actgatggcg ctaaacaggc acaaaaacgt
     5761 ctgaacgcga tgtaatgcat aacacacgac cagaagtcgc attatttctg gtcgtgtcgt
     5821 gcgaatcata agcagttgag tgatctgcat cgaaattttt gttgcgctca agtctgaaat
     5881 cagtaatata tgccgccgtt gccacgggat atcaaacaaa ccgaaagcaa cgaaaaagtg
     5941 ggtcgttagc tcagttggta gagcagttga cttttaatca attggtcgca ggttcgaatc
     6001 ctgcacgacc caccaatcgc taaggtggaa gcggtagtaa aacgtgaagg ataacgttgc
     6061 atcagcaacg gcccgaaggg cgagacgaag tcgagtcatc ctgcacgacc caccactaac
     6121 atagttagtt gtagtatcca gcgtagtatt gggtgattag ctcagctggg agagcacctc
     6181 ccttacaagg agggggtcgg cggttcgatc ccgtcatcac ccaccaccgg gtcgttagct
     6241 cagttggtag agcagttgac ttttaatcaa ttggtcgcag gttcgaatcc tgcacgaccc
     6301 accagtttta gcatcgaaga cagatgttaa gcgtgtagga taacgttgcg tcagcaacgg
     6361 cccgtagggc gagcgaagcg agtcatcctg cacgacccac cactaattac ggtgggttcg
     6421 gtggaagtag tttgtagtat ccagcgcagt atcgggtgat tagctcagct gggagagcac
     6481 ctcccttaca aggagggggt cggcggttcg atcccgtcat cacccaccac tcgggtcgtt
     6541 agctcagttg gtagagcagt tgacttttaa tcaattggtc gcaggttcga atcctgcacg
     6601 acccaccatc ctgaatgatt aaagcagcat aatcccgcaa ggggtcgtta gctcagttgg
     6661 tagagcagtt gacttttaat caattggtcg caggttcgaa tcctgcacga cccaccaatg
     6721 taaaaaagcg ccctaaaggc gcttttttgc tatctgcgat actcaaagat tcgaacctgc
     6781 agcaggtttg agttgagcgc agcgaaacaa cggagccgct cgcggcgacg gcccgaaggg
     6841 cgagcgaagc gagtcatcct gcacgaccca ccaatgtaaa aaagcgccct aaaggcgctt
     6901 ttttgctatt caggcatcct caatttcact ttgtaaacct gatgacatcg tcagagctta
     6961 ctgtgcaagc aactctacgt cggtggaatt aggcgtaatt cgagtgactt ttccccacca
     7021 ttcgactatc ttgtttagca tataaaacaa attacaccga taacagcgaa tattacgcta
     7081 atgtcggttt taacgttaag cctgtaaaac gagatggtaa gatgagcgta atgtttgatc
     7141 cagacacggc gatttatcct ttccccccga agccgacgcc gttaagcatt gatgaaaaag
     7201 cgtattaccg cgagaagata aaacgtctgc taaaagaacg taatgcggtg atggttgccc
     7261 actactatac cgatcccgaa attcaacaac tggcagaaga aaccggtggc tgtatttctg
     7321 attctctgga aatggcgcgc ttcggtgcaa agcatcccgc ttctactttg ttagtcgctg
     7381 gggtgagatt tatgggagaa accgccaaaa ttctcagtcc ggaaaaaaca attctgatgc
     7441 cgacacttca ggctgaatgt tcactggatc tcggctgccc tgttgaagaa tttaacgcat
     7501 tttgcgatgc ccatcccgat cgtactgtcg tcgtctacgc caacacttct gctgcggtaa
     7561 aagcgcgcgc agattgggtg gtaacttcaa gcattgccgt cgaacttatt gatcatcttg
     7621 atagtttggg tgaaaaaatc atctgggcac ccgacaaaca tctggggcgt tacgtgcaaa
     7681 aacagacggg tggagacatt ctatgctggc agggtgcctg tattgtgcat gatgaattta
     7741 agactcaggc gttaacccgc ttgcaagaag aatacccgga tgctgccata ctggtgcatc
     7801 cagaatcacc acaagctatt gtcgatatgg cggatgcggt cggttccacc agtcaactga
     7861 tcgctgctgc gaaaacattg ccacatcagc ggcttatcgt ggcaaccgat cggggcattt
     7921 tctacaaaat gcagcaggca gtgccagata aagagttact ggaagcacca accgcaggtg
     7981 agggtgcaac ctgccgcagc tgcgcgcatt gtccgtggat ggccatgaat ggccttcagt
     8041 ccatcgcaga ggcattagaa caggaaggaa gcaatcacga ggttcatgtt gatgaaaggc
     8101 tgcgagagag ggcgctggtg ccgctcaatc gtatgctgga ttttgcggct acactacgtg
     8161 gataacgaat aataaggcgt aacgttacgc tttgggggaa agatggattt ttttagtgtg
     8221 cagaatatcc tggtacatat accaataggg gcaggcggtt atgatctctc atggatcgaa
     8281 gcggtaggca cgatcgccgg gttgctgtgt attggccttg ccagtctgga gaagatcagc
     8341 aactacttct ttggcctgat caacgtcacc ttgtttggca ttattttctt tcagattcag
     8401 ctgtatgcca gcctgctatt acaggtgttt ttctttgccg cgaatattta cggttggtat
     8461 gcgtggtcgc gacaaaccag tcagaacgag gcggagttga aaattcgctg gttgccattg
     8521 ccgaaggcac tcagctggtt ggcggtttgc gttgtttcga ttggtctgat gacggtattt
     8581 atcaatccgg tgtttgcatt tttgacccgc gtggcagtca tgatcatgca agcattagga
     8641 ttacaggttg tgatgcctga actgcaaccg gacgctttcc cgttctggga ttcatgcatg
     8701 atggtgttat ctatcgtggc aatgattctg atgacgcgta agtatgtgga aaactggctg
     8761 ttgtgggtga ttattaacgt gattagcgtc gttatttttg cacttcaggg cgtttacgcc
     8821 atgtctctgg agtacatcat cctgaccttt attgcgctca acggcagccg gatgtggatc
     8881 aacagcgcac gtgaaagagg ctcacgcgcg ctgtcccatt aatggtgatg atgtgaatgg
     8941 ccagacaccc cttcattcag gtggcagtct ggcccatgac aaggttgata ttccatctga
     9001 atggtggcgt gctcaatctg atagtgatcc atcaggtagt gttggatctg atccaacaag
     9061 gcatcgtgat cgtgtggcgg gatcacctgc acatgcaacg tcatcaccgg cttctcgcct
     9121 accatccata catgtacatg gtgcacattg cggacttccg ggatttcccg gcacatacga
     9181 cgcttcagtt cagcgatatc cagcgatacc ggtgcacctt caagtaattc attcacgcta
     9241 tctttcaaca gtcgccacgc gctacgcaga accagaagcg acaccagtat cgagagaatg
     9301 gggtcagcag gtgtccagcc agtccagata ataatcaacg cggcaataat agccccaacc
     9361 gaacccagca ggtccccgag cacatgcagt gccgctgctc gtacgttgag gtttttctct
     9421 tcgctgccgt gatgaagtaa ccagaaagaa agtatatttg ccagcaaccc agccacggca
     9481 attgccatca tcatgccgcc ctcgaccgga cgcggcgtgc ggaagcgttc tatcgcctcc
     9541 cagacaatca aaatggtaat taccacgagg gctatggcat tcacaaacgc cgcgagcgtg
     9601 gtcagtctta gccaaccgaa agtgtggcga atggtgggag gacgacgtga aaattgcacg
     9661 gcgagtaggg cgaaaagcag ggcggcagta tcggtcaaca tatgacccgc atcggccagc
     9721 aatgccagag aaccagaaag aaaaccacca acgacttcta ccagcataaa cccggcagtc
     9781 acgccgaaag catacaacaa gcgacgagca ttattatctt ctggcaggtg tgaggacgtg
     9841 tgtgagtgtg agtgcgccat gacgagtcat cccttaatta ttctcactta actttatgac
     9901 atcatactgc ttttagaagt gaaaaattaa aagggagaga ctccgctctc ccattattgg
     9961 ctattttgca gggttactgc gtggtaccgt cggttttggt atcgacatca ttattgatgc
    10021 catcaccggt ttgtaccttt ttattgatat ccggacagcg accatctttg cacatggtgt
    10081 tcttgtgctc ttcatctttg gtcattccgt cattgttcat tgaagaacca tccgaatgca
    10141 gcattgtgcc accagaaccg gtatttaccc cgttattgtc gacgttattt ggcgcgacat
    10201 tttcacgggc gtcaggggct acctggcccg catcagctgc ggcgtttgcc tggccgttat
    10261 tagtttgcgc tccgctatcg gcagccagtg cggcaccgct ggcaaggctt agagtggcag
    10321 tcagaaataa tgtggccagt tttgtcattt tcataggatg ctcctgttat ggtcgttatg
    10381 tcggataacc tcttccaaca gtgcatttgc aggtgaatat aaggcattgg tttaagattt
    10441 cagccaggtt atgaaacgca gcagagaatc ttgaaataat taacaaacaa aggagttaca
    10501 gttagaaatt gtaggagaga tctcgttttt cgcgacaatc tggcgttttt cttgctaatt
    10561 ccaggattaa tccgttcata gtgtaaaacc ccgtttacac attctgacgg aagatataga
    10621 ttggaagtat tgcattcact aagataagta tggcaacact ggaacagaca tgaattatca
    10681 gaacgacgat ttacgcatca aagaaatcaa agagttactt cctcctgtcg cattgctgga
    10741 aaaattcccc gctactgaaa atgccgcgaa tacggttgcc catgcccgaa aagcgatcca
    10801 taagatcctg aaaggtaatg atgatcgcct gttggttgtg attggcccat gctcaattca
    10861 tgatcctgtc gcggcaaaag agtatgccac tcgcttgctg gcgctgcgtg aagagctgaa
    10921 agatgagctg gaaatcgtaa tgcgcgtcta ttttgaaaag ccgcgtacca cggtgggctg
    10981 gaaagggctg attaacgatc cgcatatgga taatagcttc cagatcaacg acggtctgcg
    11041 tatagcccgt aaattgctgc ttgatattaa cgacagcggt ctgccagcgg caggtgagtt
    11101 tctcgatatg atcaccccac aatatctcgc tgacctgatg agctggggcg caattggcgc
    11161 acgtaccacc gaatcgcagg tgcaccgcga actggcatca gggctttctt gtccggtcgg
    11221 cttcaaaaat ggcaccgacg gtacgattaa agtggctatc gatgccatta atgccgccgg
    11281 tgcgccgcac tgcttcctgt ccgtaacgaa atgggggcat tcggcgattg tgaataccag
    11341 cggtaacggc gattgccata tcattctgcg cggcggtaaa gagcctaact acagcgcgaa
    11401 gcacgttgct gaagtgaaag aagggctgaa caaagcaggc ctgccagcac aggtgatgat
    11461 cgatttcagc catgctaact cgtccaaaca attcaaaaag cagatggatg tttgtgctga
    11521 cgtttgccag cagattgccg gtggcgaaaa ggccattatt ggcgtgatgg tggaaagcca
    11581 tctggtggaa ggcaatcaga gcctcgagag cggggagccg ctggcctacg gtaagagcat
    11641 caccgatgcc tgcatcggct gggaagatac cgatgctctg ttacgtcaac tggcgaatgc
    11701 agtaaaagcg cgtcgcgggt aaggtttaat tgtcggatgc gccgtcagag tggcgtatcc
    11761 gatgaatcac cacaggcctg ataagtcgcg cagcgtcgca tcaggcaatg tgctccattg
    11821 ttagcaacaa aaatgccgac tcacttgcag tcggctttct cattttaaac gaatgacgtt
    11881 tacttcgctt taccctggtt tgcaaccgcc gctgctttcg ctgcgatctc gtcagcatta
    11941 cccagataat agcgtttcag cggtttgaaa ttctcgtcga actcatacac cagcggcacg
    12001 ccagtcggga tattaagctc aagaatctct tcttcgctca tgttatcaag atatttcacc
    12061 agcgcacgta aagagttacc gtgtgcagcg atgatcacgc gctcaccgct cttcatacgc
    12121 ggcagaatag tttcattcca gtaagggatc acgcggtcaa tggtcagcgc caggctttcc
    12181 gtcagcggca gttctttttc gctcagtttc gcgtaacgcg gatcgtgacc cggataacgc
    12241 tcatcatctt tagtcagttc cggcggagtc actgcaaaac cacgacgcca ctgtttcacc
    12301 tgctcgtcgc catacttttc agcagtttcc gctttgttca gaccctgcaa cgcaccgtag
    12361 tgacgttcgt tcagtttcca ggatttctca acgggcagcc atgcctgatc cagttcgtcc
    12421 agcacattcc acagggtatg gatagcgcgt ttcagcacag aggtgtaagc aaagtcaaag
    12481 ctgtaacctt cctctttcag cagcttacct gctgcttttg cttcgcttac gcctttctca
    12541 gacagatcca cgtcgtacca accggtgaaa cggttttctt tgttccactg actttcgcca
    12601 tgacgaacca gaaccagctt agttacagcc atatacttac tcctcaaatc attttttaat
    12661 gataataatt ctcattatat tgccgtgacg aagcaacagc aatgcttacg cataaccata
    12721 gcgaaaatag tgtcgcagtg taaggttgtt gtgaatattg agttgcaaat atgtcggtgt
    12781 ttgctggtga tttgagcaat ataaggataa agccctcatt acgagggctt caggtgctac
    12841 tcagcaataa actgatattc cgtcaggctg gaatactctt cgccaggacg caggaagcag
    12901 tccggttgcg gccattcagg gtggtttggg ctgtccggca gaaactcgct ttccagtgcc
    12961 aggccttgcc agtcggcgta aggttcagtt cctcttgatg gcgtgccgcc gaggaagttg
    13021 ccggagtaga attgcagagc cggagcggtg gtgtagacct tcagctgcaa tttttcatct
    13081 gctgaccaga catgcgccgc cactttcttg ccatcgcctt tggcctgtaa caagaatgcg
    13141 tgatcgtaac ctttcacttt gcgctgatcg tcgtcggcaa gaaactcact ggcgatgatt
    13201 ttggcgctac ggaaatcaaa agacgttccg gcgacagatt tcaggacgtc gtgcggaatg
    13261 ccgccttcat caaccggcag atattcgtcc gccagaatct gcaacttgtg attgcgcacg
    13321 tcagactgct cgccgtcaag attgaaatag acgtgattag tcatattcac cgggcaaggt
    13381 ttatcaactg tggcgcgata agtaatagag atacggttat cgtcggtcag acgatattgc
    13441 accgtcgcgc cgagattacc cgggaagccc tgatcgccat catctgaact cagggcaaac
    13501 agcacctgac gatcgttctg gttcacaatc tgccagcgac gtttgtcgaa cccttccggg
    13561 ccgccgtgca gctggttaac gccctgactt ggcgaaagcg tcacggtttc accgtcaaag
    13621 gtataacggc tattggcgat gcggttggca taacgaccaa tagaggcccc cagaaacgca
    13681 gcctgatcct gatagcattc cgggctggca cagccgagca gcgcctcgcg gacgctgcca
    13741 tcggaaagcg gaatacgagc tgaaagtaaa gtcgcacccc agtccatcag cgtgactacc
    13801 atccctgcgt tgttacgcaa agttaacagt cggtacggct gaccatcggg tgccagtgcg
    13861 ggagtttcgt tcagcactgt cctgctcctt gtgatggttt acaaacgtaa aaagtctctt
    13921 taatacctgt ttttgcttca tattgttcag cgacagcttg ctgtacggca ggcaccagct
    13981 cttccgggat cagcgcgacg atacagccgc caaatccgcc gccggtcatg cgtacgccac
    14041 ctttgtcgcc aatcacagct ttgacgattt ctaccagagt gtcaatttgc ggcacggtga
    14101 tttcgaaatc atcgcgcata gaggcatgag attccgccat caactcgccc atacgtttca
    14161 ggtcgccttg ctccagcgcg ctggcagctt caacggtgcg ggcgttttca gtcaggatat
    14221 gccgcacgcg tttcgccacg attgggtcca gctcatgtgc aacagcgttg aactcttcaa
    14281 tggtgacatc gcgcagagct ggctgctgga agaaacgcgc accggtttcg cactgttcac
    14341 gacgggtgtt gtattcgctg ccaaccaggg tacgtttgaa gttactgttg atgatgacga
    14401 cagccacacc tttcggcatg gaaactgctt tagtccccag tgagcggcag tcaatcagca
    14461 aggcatgatc tttcttgccg agtgcggaaa ttagctgatc catgatcccg cagttacagc
    14521 caacaaactg gttttctgct tcctgaccgt taagcgcgat ttgtgcgccg tccagcggca
    14581 gatgataaag ctgctgcaat acggttccga ccgcgacttc cagtgaagcg gaagaactta
    14641 acccggcacc ctgcggcaca ttgccgctga tcaccatgtc cacgccgccg aagctgttgt
    14701 tacgcagttg cagatgtttc accacgccac gaacgtagtt cgcccattga tagttttcat
    14761 gcgcgacaat gggcgcatcg agggaaaact cgtcgagctg attttcataa tcggctgcca
    14821 ttacgcgaac tttacggtca tcgcgtggcg cacagctgat cacggtttga taatcaatcg
    14881 cgcagggcag aacgaaaccg tcgttgtagt cggtgtgttc accaatcaaa ttcacgcggc
    14941 caggcgcctg aatggtgtga gtggcagggt agccaaatgc gttggcaaac agagattgtg
    15001 ttttttcttt cagactcatt tcttacactc cggattcgcg aaaatggata tcgctgactg
    15061 cgcgcaaacg ctctgctgcc tgttctgcgg tcaggtctcg ctgggtctct gccagcattt
    15121 cataaccaac cataaattta cgtacggtgg cggagcgcag cagaggcgga taaaagtgcg
    15181 cgtgcagctg ccagtgttga ttctcttcgc cattaaatgg tgcgccgtgc cagcccatag
    15241 agtaggggaa ggagcactgg aagaggttgt cataacgact ggtcagcttt ttcaacgcca
    15301 gcgccaaatc gctgcgctgg gcgtcggtca aatcggtgat ccgcaaaacg tgggctttgg
    15361 gcagcagtag cgtttcgaac ggccaggcag cccagtaagg cacgacggct aaccagtgtt
    15421 cggtttcgac aacggtacgg ctaccgtctg ccagctcgcg ctgaacataa tccaccagca
    15481 ttggtgattt ctgttcggca aaatattctt tttgcaggcg ttcttcgcgc tcagcttcgt
    15541 taggcaggaa gctatttgcc caaatctgac cgtgcggatg cgggttagag cagcccatcg
    15601 cagcgccttt gttttcaaaa acctgcaccc atgggtacgt tttccccagt tctgcggttt
    15661 gctcctgcca ggttttgacg atttccgtca atgctgcaac gctgagctct ggcagcgttt
    15721 tactgtgatc cggtgaaaag cagatcaccc ggctggtgcc gcgcgcgctc tggcaacgca
    15781 tcaaaggatc gtgactttct ggcgcatctg gcgtgtcaga catcaaagcc gcaaagtcat
    15841 tagtgaaaac gtaagtcccg gtgtaatcgg ggtttttatc gcctgtcacc cgcacattac
    15901 ctgcgcagag gaagcaatct ggatcgtgcg caggtaacac ctgtttggct ggcgtttcct
    15961 gcgccccctg ccaggggcgc ttagcgcggt gcggtgaaac cagaatccat tgcccggtga
    16021 gcgggttgta gcggcgatgt ggatgatcaa cgggattaaa ttgcgtcatg gtcgttcctt
    16081 aatcgggata tccctgtgga tggcgtgact gccagtgcca ggtgtcctgc gccatttcat
    16141 cgagtgtgcg cgttacgcgc cagttcagtt cacggtcggc tttgctggcg tccgcccagt
    16201 aggccggaag gtcgccttcg cgacgcggtg caaaatgata gttaaccggt ttgccgcagg
    16261 ctttgctgaa ggcattaacc acgtccagca cgctgctgcc tacgccagca ccgaggttgt
    16321 agatgtgtac gcctggcttg ttcgccagtt tttccatcgc cacgacgtga ccgtccgcca
    16381 gatccattac gtggatgtaa tcgcgtacgc cagtaccatc ttcggtcgga taatcgttac
    16441 caaaaatcgc cagcgagtcg cgacggccta cagcaacctg ggcgatgtat ggcatcaggt
    16501 tattcggaat gccttgcgga tcttcgccca tatcgcccga cggatgcgcg ccaaccgggt
    16561 tgaagtagcg cagcagggca atgctccagt ccggctgggc tttttgcagg tcggtgagga
    16621 tctgttccac catcaatttg cttttgccat aagggctttg cggtgtgccg gtcgggaagc
    16681 tttcaacgta tggaattttg ggctgatcgc cataaacggt agcggaggag ctaaaaataa
    16741 agtttttgac gttagcggcg cgcatggcgc taatcaggcg cagagtgccg ttgacattgt
    16801 tgtcgtaata ttccagcggt ttttgtaccg attcgccgac ggctttcagc ccggcgaagt
    16861 ggatcacggt gtcgatagcg tgatcgtgca gtatctcggt catcaacgct tcgttacgaa
    16921 tatcgccttc aacaaacgtt ggatgtttgc cgcctaaacg ctcgataaca ggcagtacgc
    16981 tgcgcttact gttacagagg ttatcaagaa tgatgacatc atgaccgttt tgcagtaatt
    17041 gcacgcaggt atgacttcca atgtaaccgc taccaccggt aaccagaact ctcataattc
    17101 gctccattag gcttatggta tgaaataacc atagcataac aaagatgcga aaagtgtgac
    17161 atggaataaa ttagtggaat cgtttacaca agaatttagc cgttttttat gcgcgattaa
    17221 gtgattataa aacagagggt ttatgaatga ttgcgctttt tatctgaaaa aagaagcggt
    17281 ttcatgcctg catgcgtcga accgttggcc ggagagggcg cttaggccgc ctccggcaag
    17341 gtcagcacta ccgactcaat atatttttgt cagcacatag cgatagagtc caccgtccgg
    17401 cacgaactca aggcgatggg taatacaggc aggcgcatct tcagcgtggt gcgaaacaaa
    17461 caacaattgc gtttcacctt cgctaatcag cacatcaaca aaacggcgga taagctggcg
    17521 attgagcgga tcaagcccct gtagtggttc atcgagaata agcaacgtcg gatgtttcac
    17581 cagtgcgcgg acgatcagcg ccagacgctg ctgtccccag gaaagactat ggaaaggagc
    17641 gtcagccgtg cgtttatcaa tgccgagaat atccagccac tgctgcacca gtttttgctg
    17701 gcgatccgaa acggcctgat aaatgccaat cgaatcaaaa tagccagaaa gaatcacatt
    17761 acgcacagta gtactgaccc ggtaatccag atgcaaacta ctgctgacgt aaccgatatg
    17821 ctttttgata tcccagatgg tttcgccgct gccgcgacgt cgtccgaaaa gcgtcaaatc
    17881 gttgctgtaa ccttgcggat gatcgccagt aaccaggctt aataacgtcg attttcctgc
    17941 accatttggc ccgacaattt gccagtgttc gcctggattc acctgccagc taaggttatt
    18001 aagaatgggg cgatcgttat aagaaaccac gccattgttc agcacaatgc gcggttcgtt
    18061 ggcgggtaag gcgtgacgtg ctgaaggttc atccggctcc ggcagttgca caccttcaag
    18121 ctgttcacta tgcgccagtt gcgcgacgag tgcttgttgg agcagttcct ctttagcgcc
    18181 agtttccgct aacgtgcaat ccgccagcac gccagcaaac tggacaaact ccgggatctc
    18241 atcgaagcga ttgagcacca gtaccagagt aataccggac tgatgtaacg aggcgagtcg
    18301 ctcagccagc tgctgacgtg aggcaacatc caggccatcg aacggctcat caagaatcaa
    18361 caagtcaggc tccgacatca gcgcctgaca cagcaggctt tttcgcgtct cgccagtgga
    18421 aaggtattta aagcgtcggt cgaggagggc ggtaataccg aactgctgcg ccagttgcat
    18481 gcaacgcggt gcatccttta cttcatcctg aatgatctca gccgtagtgc gtccggtgtc
    18541 atcttcgcca gggccgagca tatcggtgtt attccgctgc cattcgtcgc tgacgagttt
    18601 ttgcaattgc tcgaaggaga gacgagtgat gtgggaaaac tggctttgcc gttcaccttt
    18661 caaaagcgga agttcccccg ccagcgcgcg ggccagggcc gatttcccgc ttccattcga
    18721 accgacaaac gcccaactat cacccgcgtt taacgttagc tgaggcaatt gcagcgtttt
    18781 tgtgtcgcta agacgaaacg tgccttgcaa aatttgcaac gatgacattt tatatcccat
    18841 tttgtgcagc gattactgac agggatacgt gtttcataac aaattgtcaa cacgcttagc
    18901 acagcgtggc gataatcacg ctgtcggcat taaagtaggc cgtgacattc tgcccttgct
    18961 gaagagaagt cgcttcattt accggcactg tggcgcacag tgtttgcccg tcgggtagcg
    19021 ccattaatac ttcgcactgc tctgcgccgc gctcaatatg actaataata cccggtaatt
    19081 ggttgtcagc gttttgcgcg accgcctcgt cctgagtaat acctacccac ggcgctttta
    19141 gcaatatcaa cacttctttg ccttcatcca gccccagacg cgcgccgctt tgtgcggtaa
    19201 ttgcgacttt caggcgtgtt tttccgtcag ccagtaagac atcaacatgc tgttgaacgt
    19261 catcatgatc gcgggcggtg atggtgccga accactggtt acgggcgctg gtttgcagtg
    19321 aaaaacgtga gatcgcggcc agcaggctgt tcagcggcag ggcgtcatcg tcacttaaca
    19381 catcaaaggc tttttgctgg atttgcgcca gtaagtcata gagctgaatc agtcgctgac
    19441 catagcgggt cagtactgcg ccgccgccac ctttaccgcc tgttgcgcgc tcgaccagaa
    19501 tatgctcact taactgattc atctcgttaa tggcatccca ggcgctttta tagctgatac
    19561 cggcatcttt cgctccctgg ctaatggaac cggaaagcgc aatgtgtttt agtagcgaaa
    19621 tccggcgcgg gtcggcgaat aatttttgtt ggagcttaag ggtgagaagg atttcggcct
    19681 gcataacaat gtcctggcaa aagtcttatt gtgacggaaa acgaacgcca cgcaaagctg
    19741 accgcacaaa aggggagtgc ttttctgtgc ttagcggtta gaatagtctc atgactatat
    19801 ctggagttga ccatgttaga gttattaaaa agtctggtat tcgccgtaat catggtacct
    19861 gtcgtgatgg ccatcatcct gggtctgatt tacggtcttg gtgaagtatt caacatcttt
    19921 tctggtgttg gtaaaaaaga ccagcccgga caaaatcatt gattccctga atgcccgctt
    19981 agtcgggcat tttctttttc tcaacttcct gcttttcctg ccgatatttt ttcttatcta
    20041 cctcacaaag gttagcaata actgctggga aaattctgag ctagccgtta tattgtcacc
    20101 tatataacgt tacattaagg ggttaccaat ggctcgtaaa tggttgaact tgtttgccgg
    20161 ggcggcactc tctttcgctg ttgctggcaa tacactggca gatgaaggga aaatcacggt
    20221 gttcgccgcc gcatcactga ctaacgcaat gcaggacatt gctacgcagt ataaaaaaga
    20281 aaaaggcgtg gatgtggttt cttctttcgc ttcgtcatct actctcgccc gtcagattga
    20341 agcgggtgcg cctgcggatc tgtttatttc tgccgatcag aaatggatgg attatgcagt
    20401 tgataaaaaa gcgatcgata ctgctacgcg tcagacactg ctcggcaata gcctggtcgt
    20461 tgtagcaccg aaagccagcg agcagaaaga tttcaccatc gacagcaaaa ccaactggac
    20521 ttcgctgctg agtggcggtc gtctggcggt tggcgatccg gaacatgttc ccgctggcat
    20581 ttatgcaaaa gaagcactgc aaaaactggg cgcatgggat acgctctctc cgaaactggc
    20641 tccggcggaa gatgttcgtg gcgcgctggc gttggtcgaa cgcaatgaag cgccgctggg
    20701 catagtttac ggttctgacg cagttgccag caaaggggta aaagtggtcg ccaccttccc
    20761 ggaagattcg cataaaaaag tggaataccc ggttgctgtt gtggaagggc ataacaatgc
    20821 gacagtgaaa gcattttatg attatttgaa agggccgcag gctgcggaaa tctttaaacg
    20881 ttacggattt acaaccaagt aatgatactg accgatccag aatggcaggc agttttatta
    20941 agcctgaaag tttcttccct ggctgtgctg tttagcctgc cgtttgggat cttttttgcc
    21001 tggttactgg tgcgttgcac gtttccgggc aaagctctgc tcgacagcgt actgcatcta
    21061 ccgctggtgt taccgcccgt ggtcgtcggt tacttattat tagtttcgat gggacggcgc
    21121 gggtttatcg gtgaacgtct gtatgactgg tttggcatta ccttcgcctt tagctggcgc
    21181 ggcgcggttc tcgctgccgc cgtcatgtcg tttccgctga tggtgcgggc aattcgtctg
    21241 gcgctggaag gggttgatgt caaactggaa caggccgcaa gaacactggg ggccgggcgc
    21301 tggcgcgttt tctttactat cacgataccg ctgaccttac cgggaattat tgttggtacg
    21361 gtactggctt ttgctcgttc tctcggtgaa tttggcgcaa ccatcacctt tgtgtcgaac
    21421 attcctggtg aaacgcgaac cattccttct gccatgtata ccctgatcca gacccccggc
    21481 ggggaaagtg gcgcggcgag actgtgcatt atttctattg cgctggcgat gatctccctg
    21541 ttgatttcag aatggctggc cagaatcagc cgtgaacggg cggggcgcta atcatgctgg
    21601 aactgaattt ttcccagacc ttgggcaacc attgcctgac cattaatgaa acgctgcccg
    21661 ccaatggcat cactgctatc tttggcgtct ccggtgccgg aaaaacttcg ctgattaacg
    21721 ccatcagtgg actgacgcgc ccgcaaaaag ggaggattgt cctcaatggt cgggtactaa
    21781 atgatgccga aaaaggtatc tgcctgacgc cagaaaagcg tcgcgttggc tatgtttttc
    21841 aggatgcgcg gctgtttccg cattacaaag tgcgtggcaa tctgcgctac ggcatggcga
    21901 aaagcatggt cgatcagttc gataagctgg tggcgctttt aggcattgaa ccgttgcttg
    21961 accgtttacc aggcagcctg tccggaggcg aaaaacagcg cgtagcgatt gggcgggctt
    22021 tgctgacggc accagaattg ctgttgctgg atgaaccgct ggcgtcactg gatattccgc
    22081 gtaaacgcga actgttgcct tatctgcaac gtctgacgcg ggaaatcaac atcccgatgt
    22141 tgtatgtcag ccattcgctg gatgagatcc tccatctggc agacagagtg atggtactgg
    22201 aaaacggtca ggtgaaagcc tttggcgcac tggaagaagt ctggggtagt agcgtgatga
    22261 atccgtggct gccgaaagag caacaaagta gcattctgaa agtgacggta cttgagcatc
    22321 atccgcatta cgcgatgact gcgctggcgc tgggcgatca gcatttgtgg gtcaataagc
    22381 tggacgaacc gctgcaagct gcgctacgta tccgcattca ggcttccgat gtttctctgg
    22441 ttttacaacc gccgcagcaa accagcattc gtaacgtatt gcgggcaaaa gttgttaata
    22501 gttatgacga caacggccag gtggaagtgg aactggaagt cggaggtaaa acgctgtggg
    22561 cgcgtatcag cccgtgggcc agggatgaac tggtgatcaa acctggcctg tggctgtacg
    22621 cgcaaattaa aagtgtgtcg ataaccgcct gattaaatca ggtggctata aatgaactgg
    22681 gcaatgctgt cggtggtgtt atcaccaatc acaatgttgg cgcgcgcttt taccgcgtca
    22741 tcggcgttgc ccatcgccac gcctgtacca gcggcctcca gcatactgat atcattaaag
    22801 ttatcgccga acgccacgac gttttccatc gaccagcctt gcgcctcaac ccatttcgtc
    22861 aaacgtttac ctttgctgtt gccgccgcgt gcaatatcaa cctgatcgtg ccaggaccat
    22921 tcacactcca gtcccagttc atgttcgaca tgcttaccaa aatgctgcaa ttgcggcagg
    22981 tcatcgtgcg tcagggcgaa cttccatacg gcgttaactt gttgcgccgt ttcagccaga
    23041 gaagcgactt gtgtgaaagt cggacgctgt tccggcggca gggtttgcgc ccagttagac
    23101 gtgcgaatga catgcccggt cgggtgctca tagaccattg catcatccac atacatcaga
    23161 ccgtgaatgt ggtgttcatt cagcatctca atgagttgca gggctttatt aacgggcatt
    23221 gggtccgctt ccagcacggt ttttgcatga taatcataca aataggtgcc attacagcaa
    23281 atagcaggtg tatccagcgc cagcgcctga taaaaaggat gaatagcgac gtgatggcga
    23341 cctgtgacga tgattaattg atagcctgct tcgcgagcgc gggccagggc ttctatcgat
    23401 gaaggaagca gggtcttttt cggggtcaat aaggtgccgt ctaagtcgag agcaatcacg
    23461 cgtgtggtca tggcgtattc cagattaagg ttaagaattt tcgtctgcgc gaatggtaca
    23521 ccgataccac tatcggacaa aattctgcgt tttaattcag cattcaccgc caaaagcgac
    23581 taattttagc tgttacagtc agttgctaaa tgcaaaggag cattcatgaa gcaaacagtt
    23641 tatatcgcca gccctgagag ccagcaaatt cacgtctgga acctgaatca tgaaggcgca
    23701 ctgacgctga cacaggttgt cgatgtgccg gggcaggtgc agccgatggt ggtcagcccg
    23761 gacaaacgtt atctctatgt tggtgttcgc cctgagtttc gcgtcctggc gtatcgtatc
    23821 gccccggacg atggcgcact gacctttgcc gcagagtctg cgctgccggg tagtccgacg
    23881 catatttcca ccgatcacca ggggcagttt gtctttgtag gttcttacaa tgcgggtaac
    23941 gtgagcgtaa cgcgtctgga agatggcctg ccagtgggcg tcgtcgatgt ggtcgagggg
    24001 ctggacggtt gccattccgc caatatctca ccggacaacc gtacgctgtg ggttccggca
    24061 ttaaagcagg atcgcatttg cctgtttacg gtcagcgatg atggtcatct cgtggcgcag
    24121 gaccctgcgg aagtgaccac cgttgaaggg gccggcccgc gtcatatggt attccatcca
    24181 aacgaacaat atgcgtattg cgtcaatgag ttaaacagct cagtggatgt ctgggaactg
    24241 aaagatccgc acggtaatat cgaatgtgtc cagacgctgg atatgatgcc ggaaaacttc
    24301 tccgacaccc gttgggcggc tgatattcat atcaccccgg atggtcgcca tttatacgcc
    24361 tgcgaccgta ccgccagcct gattaccgtt ttcagcgttt cggaagatgg cagcgtgttg
    24421 agtaaagaag gcttccagcc aacggaaacc cagccgcgcg gcttcaatgt tgatcacagc
    24481 ggcaagtatc tgattgccgc cgggcaaaaa tctcaccaca tctcggtata cgaaattgtt
    24541 ggcgagcagg gactactgca tgaaaaaggc cgttatgcgg tcgggcaggg accaatgtgg
    24601 gtggtggtta acgcacacta accgctgatt taaccggcgc agtctctcct gcgccggtgt
    24661 attaacctat ctcctgtaac gcatgtctct ggcgttcgac gatattggtc cacaaattgt
    24721 ctttatcgtc agtccacaaa ttaatcagca aggcaaaaaa gcgttcagct gccggagaaa
    24781 gcacggcatc tttgcggcga ataatcccca atgtccgacg tataacgggt tccaccagtg
    24841 ggataccaat aatcgaagaa tagggcgcat ggggcattgc cagaccagga agcgccgaaa
    24901 tccccagtcc tgcctccact agtcctaatg acgtcgaaag atgacgcact tcgtaaaacc
    24961 agtccagctt ccagggcttg tcggctagct gttgttctat caacaatcgg ttgccgctgg
    25021 aggaacgtac gccaatcatt ttgtaaccca ctaactcctg ccattctaca agctgctttt
    25081 tggccaatgg atggtcacgc cgacaggcca gcacgaatgg ctcgttgacc agtggggtaa
    25141 aatcaatgga tgaatttgtg acgttgttca tgtttatgcc aaagtctgat tcgttacaca
    25201 gcaccgattc catgcaatta ttcgtgcctt gttccagaat ccgcacttta atattgggat
    25261 acagctcatt aaatttaccg attgccagcg gtaaaaaata aaatactgcg gtcggaatac
    25321 acgctaacgt caccatacca cgatgataag cattcatatc acgaatatta aaaagcgttt
    25381 catcgaattt ctttattaat tcccgcgcct ccgggagcaa cctttttcct gcttttgtta
    25441 acgtaacctt gcgagttgta cgctcaaata gttgaatatg taaatcttcc tccatctttt
    25501 ttattcttcg cgttaatgca ggttgcgtaa tattgagtaa tttagcggca ttgttaaatg
    25561 aactggactc tgccagtatg acaaatgcct tcatacttga taattcatgc ttcattatta
    25621 ctccggaaaa tggaagcgac gattttgggt ggctggtcgt taaaaatttt aactgcattt
    25681 agccaactta aattaatgaa aaaatgttat taatcgttga gctaaagtca ttagagatgc
    25741 tttgtcctta atgtaaccat atcgcaataa gttatgtttt taaattgagg gcattattat
    25801 gaaaaaaata ccctgcgtga tgatgcgagg tggaacctcg aggggcgcgt tcttgttagc
    25861 ggaacattta cccgaagatc aaacgcagcg cgataaaata ttgatggcaa ttatgggttc
    25921 cggtaacgat ctggaaattg acggtattgg cggcggtaat ccgctgacca gtaaagtcgc
    25981 cattattagc cgttccagcg atccgcgtgc tgatgtcgat tatctgtttg ctcaagttat
    26041 tgtccatgag caacgtgtcg ataccacgcc taattgtggc aatatgctgt ctggtgttgg
    26101 ggcattcgcc attgaaaatg gtttgattgc agcgacatca ccggtcaccc gtgtgcgtat
    26161 ccgcaacgtc aatacgggta cgttcatcga agctgatgtg caaacgccaa atggtgttgt
    26221 cgagtacgag ggtagtgcca gaattgacgg cgtaccgggt actgccgcac cggttgcgct
    26281 cactttcctg aatgccgctg gaaccaaaac cggaaaagtt ttcccgactg ataatcagat
    26341 tgattatttt gacgatgtcc cggtgacctg tatcgatatg gcgatgccag tcgtcattat
    26401 tccggctgaa tatctgggaa aaacaggtta tgaattaccg gcggaactgg atgccgacaa
    26461 agcattatta gcccgcattg aatctatccg tctacaagcg ggtaaagcaa tgggcttagg
    26521 tgatgtcagt aatatggtta tccctaaacc tgtgcttatt tctccagcgc agaaaggcgg
    26581 ggcaattaat gtgcgttatt ttatgccgca ttcttgccat cgcgcgctgg cgataaccgg
    26641 tgctattgct atttccagta gttgtgcatt ggaaggcacc gtcacccgac aaatcgtccc
    26701 ttctgtagga tacggcaata tcaatattga acaccccagt ggtgcgctcg acgttcattt
    26761 aagtaatgaa ggtcaggatg ccacgacgtt acgcgcatct gttattcgga cgaccagaaa
    26821 aatattttcc ggtgaagttt atcttccctg aaaaaattcg ttgtcaagat aaggataatc
    26881 aataaaggac ttctgtatga gtcatacaga aagaacagga ttttaaatga ataagaaatc
    26941 gttatggaag ctaattctga tattagcgat cccatgtatt attggtttta tgccagctcc
    27001 ggcaggatta agcgaactgg cgtgggtgct ttttggtatt tacctggcgg ccattgtggg
    27061 gctggttatc aagcctttcc cggaacctgt cgtactgtta attgccgttg ctgcctccat
    27121 ggtggtggtc ggtaacttat ccgacggtgc gtttaagacc accgccgtat taagcggtta
    27181 ctcttcaggt actacctggc tggtgttctc ggcgtttacc ttaagcgccg catttgtgac
    27241 caccggttta ggtaaacgta ttgcctatct gctgattggt aaaatcggta acaccacgct
    27301 gggtctgggt tacgttacgg tattcctcga tctggtactg gctccggcaa caccgtctaa
    27361 caccgcgcgt gcgggcggta ttgtgttacc gatcatcaac agcgtggcgg tggctttggg
    27421 gtccgaaccg gaaaaaagtc cgcgtcgtgt cggacattac ctgatgatgt ccatttacat
    27481 ggtcaccaaa accaccagct atatgttctt taccgcaatg gcggggaaca ttctggcgct
    27541 gaaaatgatc aacgacattc tgcacctgca aattagctgg ggtggatggg cactggccgc
    27601 cggattgccg ggcatcatta tgctgctggt caccccactg gtgatttata ccatgtatcc
    27661 gccagaaatt aagaaggtgg ataataaaac catcgctaaa gcgggccttg ccgaactggg
    27721 accgatgaaa atccgcgaaa aaatgctgct cggtgtcttt gtgctggcgc tactgggctg
    27781 gattttcagt aagtctctgg gggttgatga atccactgtg gcaatcgttg ttatggcgac
    27841 catgctgctg ctgggtatcg ttacctggga agacgtggtt aaaaataaag gcggctggaa
    27901 taccttaatc tggtacggcg gtattatcgg attaagctcc ttattatcga aagttaaatt
    27961 cttcgaatgg ttagctgaag tctttaaaaa taacctggca tttgatggtc acggtaacgt
    28021 tgctttcttc gttattattt tcctcagcat tatcgtgcgt tatttcttcg cttccggtag
    28081 tgcctatatc gttgctatgt taccggtatt tgccatgctg gcgaacgtct ccggcgcacc
    28141 gttaatgtta accgcgctgg cactgttgtt ctccaactcc tatggcggca tggttactca
    28201 ctatggcggc gcggcaggtc cggtcatctt tggcgtgggt tataacgata ttaaatcctg
    28261 gtggttggtc ggtgcggtac tgacgatatt aaccttcctg gtgcatatca ccctcggcgt
    28321 gtggtggtgg aatatgctga tcggctggaa catgctgtaa atattctcgt catacttcaa
    28381 gttgcatgtg ctgcgtctgc gttcgctcac cccagtcact tacttatgta agctcctggg
    28441 gattcactcg cttgtcgcct tcctgcaact cgaattattt agagtatatc catttattat
    28501 ctttctgcgc acttcacggt gcgcagatat ctggagcatt tgatgatcaa gttatctgaa
    28561 aaaggcgtgt ttctcgccag taataacgaa ataattgccg aagaacattt caccggcgaa
    28621 attaaaaaag aagaagccaa aaaaggcact attgcctggt ctattctctc ttcgcataat
    28681 acgtccggaa atatggataa acttaaaatt aagtttgatt cattagcctc tcacgatatt
    28741 acctttgttg gtattgtaca gaccgctaaa gcgtccggta tggaacgttt cccgctgccg
    28801 tatgtgctga ccaactgcca taactcactc tgcgccgtcg gcggcactat taacggtgat
    28861 gaccatgttt ttggtttatc ggcggcccag cgttatggcg gtatttttgt gcctccgcat
    28921 attgcggtca tccatcaata tatgcgtgag atgatggcag gcggcggcaa aatgatcctc
    28981 gggtcagaca gccacacccg ttacggtgca ttagggacaa tggcagtcgg tgagggcggc
    29041 ggtgagttgg taaaacagct gcttaatgac acctgggata tcgactatcc gggcgtggtt
    29101 gcggtgcatc tgaccggaaa acctgcgccg tatgtggggc cacaggatgt ggcgctggct
    29161 atcattggcg cggtgttcaa aaacggttac gtcaaaaaca aagtcatgga gttcgttgga
    29221 ccgggcgtta gcgcgctctc taccgatttc cgtaacagcg ttgacgtgat gaccacggaa
    29281 accacctgtt taagctccgt ctggcaaact gatgaagaag tgcataactg gctggcgctg
    29341 cacggtcgcg gccaggatta ctgccagctt aaccctcaac cgatggcgta ctacgatggc
    29401 tgcatcagcg ttgatttaag cgccatcaaa ccaatgattg cgctgccgtt ccacccgagc
    29461 aacgtgtata aaatcgacac actgaaccag aacctgaccg acattctgcg tgagattgaa
    29521 attgagtccg aacgcgtggc gcacggtaaa gccaaactct cgctgctgga taaagtggaa
    29581 aatggtcgcc tgaaagtgca gcaggggatt atcgcgggct gttctggcgg taactacgaa
    29641 aacgtcatcg cggcggcgaa tgcactgcgc ggtcaatcct gtggcaatga caccttctcg
    29701 ctggcagttt acccgtcatc acagccggtg tttatggatc tcgccaaaaa aggtgtggta
    29761 gcagatttga ttggcgcagg cgcaatcatc agaaccgcgt tctgcggccc atgctttggc
    29821 gcgggcgata cgccaatcaa caacggtttg agtattcgcc acaccacgcg taacttcccg
    29881 aaccgcgaag gctctaagcc agctaatggg cagatgtcag cggtggcgtt gatggacgct
    29941 cgttctatcg ctgcgactgc ggcaaacggt ggctatttaa cctctgccag cgaacttgat
    30001 tgctgggaca acgtgccgga gtacgccttc gatgtaacgc cgtataaaaa ccgtgtttat
    30061 cagggctttg tgaaaggggc aactcagcaa ccgctgattt acggaccgaa cattaaagac
    30121 tggccggaat tgggtgcgct gactgacaat atcgtcctga aagtgtgctc gaagatcctc
    30181 gacgaagtga ccaccaccga cgaactgatt ccttccggtg aaacctcttc ttatcgttca
    30241 aatccgattg gtctggcgga gtttaccctg tcacgccgcg atcccggtta tgttggcaga
    30301 agtaaagcga ctgctgagct ggaaaatcag cgtctggcgg ggaatgtcag cgagctgaca
    30361 gaggtgtttg cgcgcattaa gcagattgct ggtcaggagc atattgatcc gctgcaaact
    30421 gaaattggca gcatggtata tgcggtgaaa ccaggcgatg gttctgcgcg tgaacaggcg
    30481 gcgagctgcc agcgtgtgat tggcggtctg gcgaatattg ccgaagagta cgcgactaaa
    30541 cgctaccgtt ctaacgtcat caactggggg atgttaccgc tgcagatggc ggaagtgcca
    30601 acctttgaag tgggggatta catttacatc cctggcatta aagcggcgct ggataatccg
    30661 ggtacgacgt ttaaaggtta tgtgatccat gaagatgcgc cggtaacgga aattacgctc
    30721 tatatggaaa gtctgactgc tgaagagcgc gagattatca aggcgggtag tttgattaac
    30781 ttcaataaaa accgtcagat gtaaaaagcg ccatgtgaat gtaggtcgca ttcggcactt
    30841 attgtcggat gcgatgcttg cgcatcttat ccgacctacg aatcgtatcg aatctgtagg
    30901 ccagataagg cattttcgca gcatccggca cttattgtcg gatgcgatgc ttgcgcatct
    30961 tatccgacct acaaatcgca tcgaaccgta ggccggataa ggcgtttacg ccgcatccgg
    31021 caaatagtta attgctctta cttcttcgcc tctgcaacca ctttactacc cacgccgcgg
    31081 ttattgtatt cccacatgcg gttgtagtta gtgtcattca gattgcgctg tatttcgtcg
    31141 ttatcatcta cgctgccggt attacccgca aacggacgat tagagatcac cgcatcggcc
    31201 cacggtttag ccgtgttaaa accttcgttg atggcgctat cacggatcac cacctgaccg
    31261 ttggtattgg catcaacatc cagcgagcgg cccagttgcg ccacaccatc accgaaagca
    31321 ttgaaacggc tgtttacggc gaggaaaccg tagtaaatgt tggacagcgt agccggtgca
    31381 aacacatacg cttcttgctg agtacgtgag ttcaccacgc ggaattcggt gttatcgaac
    31441 accactgcgc cgcgaccaga aacgatatcc acatcccctt caatgtagct gttggtcacc
    31501 agcgtacgcg gctgacggtt ggtttccaga cggttctgca caccgctgtt ggtgacaaag
    31561 aaggtgttct gacgaccgag aatgttaacg ttgttaatct gtacctggtc accatcagta
    31621 cgcagtgcca ccgccggatg gttacctgca tctacgctat cgcccagcgt gttttcgatg
    31681 gtcagatttt gcagttgcag gccattgttt tgtgaccaga agaccgcaga gcagagaaca
    31741 ccgatactgt cgctgcgttt gctctggcag ctatcgtaca tataccacgc tggtttacct
    31801 ggcatatatt tgccgcgcgg gttgacgtcg tgacgccagt cggcagggct catgccacca
    31861 tcaagggaaa gcccaatctt cacatcaatc ggtttttcac ctgtaccgta cagagtaatt
    31921 ccacccggag cggcagggac atataccgtt ccctgatact caccaggcat cacggcaata
    31981 tactggcgct tgttggtacg cttgataatt gccgcatcta ccgccgcctg aatcgtggta
    32041 tgcgttacac cttgagtgcc cgccgggccg acaacaaagt caggttgcgc aggcagggta
    32101 atcggggaag gattccacgc tgcagcacct ggtgtcaggg atgcaaaata gtgttgagca
    32161 tcgaaattct gcgcttcttt tgccgacaga atcgggcgag aagaggtacc aggcgcggtt
    32221 tgatcagaag gacgttgatc gggcggggtt gagctacagg cggtcagcgt cacgccaaaa
    32281 gccaatgcca gcgccagacg ggaaactgaa aatgtgttca caggttgctc cgggctatga
    32341 aatagaaaaa tgaatccgtt gaagcctgct tttttatact aacttgagcg aaacgggaag
    32401 gtaaaaagac aaaaagttgt ttttaatacc tttaagtgat accagatggc attgcgccat
    32461 ctggcagagt gattaactaa acatcgcagt aatcgaggcg cttgccagag agtggaaatg
    32521 aacgttaaac ccgaccatcg cgccgctggc accttcatcg acatcaatac gttctacatc
    32581 cagcgcgtga acggtaaaaa tgtagcgatg agtttcgcct ttcggcggtg ctgcgccatc
    32641 gtacccggtt ttaccaaagt cggtacgcgt ctgcaaaacg ccgtctggca ttgctaccag
    32701 accagagcca aacccttgcg gtaatacgcg ggtatcagcg ggtaagttaa caactaccca
    32761 gtgccaccag ccggagccgg ttggcgcatc cgggtcgtag caggtgacaa caaaactttt
    32821 cgttcccgca ggaacatcat cccacgccag atgcggtgaa atattatcgc catcgtaacc
    32881 catgccgtta aagacatgac gatgcggcaa tttatcgcca tcgcgcagat cgttactgat
    32941 gagtttcatg aaccctcctt tcttgtttgc agaaagtgta gccagaaacc ctcacgcgga
    33001 cttctcgtta ttggcaaaaa aatgtttcat cctgtaccgc gcggttaacc gctgcggtca
    33061 gacgctgcaa ctgttgcggg agaataatat agggcggcat caggtaaatc agtttgccaa
    33121 aaggccggat ccagacaccc tgttcgacaa agaatttttg cagcgccgcc atattcaccg
    33181 gatgagtggt ttcgactacg ccgattgccc ccagcacgcg cacatcggca accatttcgg
    33241 catcacgggc gggggcaagt tgctcgcgca gctgtacttc aatagccgcc acctgttgct
    33301 gccagtcgcc agattcgaga atcgccaggc tggcgtttgc tgccgcgcag gccagcggat
    33361 tgcccataaa agttggccca tgcataaagc aaccggcttc gccgttactg atggtttccg
    33421 caacctcgcg cgtggtgagc gtggcggaaa gggtcattgt gccgccggtt aaggctttac
    33481 cgaggcacaa aatgtccggc gcgatttctg catgttcaca ggcaaacagt ttgccggtac
    33541 gaccaaatcc ggtggcgatc tcgtcggcaa tcagcaagat accttcgcga tcgcacattt
    33601 tgcggattcg ttttaaccat tccggatggt acatgcgcat cccgcctgcg ccctggacaa
    33661 tcggttcaat gatcaccgcc gcgatttcat gacgatgcgc cgccatcagg cgggcaaagc
    33721 caaccatatc acgttcatcc cattcaccat ccatgcggct ttgcggggcg ggagcaaaca
    33781 agttttctgg cagatagcct ttccacaggc tgtgcattga gttgtccgga tcgcacaccg
    33841 acatcgcgcc aaaggtatcg ccatgataac cattgcggaa ggtcagaaaa cgctggcgcg
    33901 cttcgccttt ggcctgccag tactgcaacg ccattttcat cgccacttcc accgctacgg
    33961 agccggagtc cgcgagaaaa acgcattcca gcggttgcgg cgtcattgcc accagtttgc
    34021 ggcatagctc gatggcgggc gcatgggtga taccgccaaa catcacatgc gacatagcat
    34081 caatttgcga cttcatcgcc gcattaagct gcgggtgatt atagccgtgg atcgccgccc
    34141 accaggacga cataccgtca accaggcgtc tgccgtcaga caaaattagc tcgcaacctt
    34201 cggcgctcgc gaccggataa accggcagag gggaggtcat ggatgtgtat gggtgccaga
    34261 tatggcgttg gtcaaaggca agatcgtccg ttgtcataat cgacttgtaa accaaattga
    34321 aaagatttag gtttacaagt ctacaccgaa ttaacaacaa aaaacacgtt ttggagaagc
    34381 cccatggctc accgcccacg ctggacattg tcgcaagtca cagaattatt tgaaaaaccg
    34441 ttgctggatc tgctgtttga agcgcagcag gtgcatcgac agcatttcga tcctcgtcag
    34501 gtgcaggtca gcactttgct gtcgattaag accggagctt gtccggaaga ttgcaaatac
    34561 tgcccgcaaa gctcgcgcta caaaaccggg ctggaagccg agcggttgat ggaagttgaa
    34621 caggtgctgg agtcggcgcg caaagcgaaa gctgcgggat cgacgcgttt ctgtatgggc
    34681 gcggcgtgga agaatcccca cgaacgcgat atgccgtacc tggaacaaat ggtgcagggg
    34741 gtaaaagcga tggggctgga ggcgtgtatg acgctgggca cgttgagtga atctcaggcg
    34801 cagcgcctcg cgaacgccgg gctggattac tacaaccaca acctggacac ctcgccggag
    34861 ttttacggca atatcatcac cacccgcact tatcaggaac gcctcgatac gctggaaaaa
    34921 gtgcgcgatg ccgggatcaa agtctgttct ggcggcattg tgggcttagg cgaaacggta
    34981 aaagatcgcg ccggattatt gctacaactg gcaaacctgc cgacgccgcc ggaaagcgta
    35041 ccaatcaaca tgctggtgaa ggtgaaaggc acgccgctgg cggataacga tgatgtcgat
    35101 gcctttgatt ttattcgcac cattgcggtc gcgcggatca tgatgccgac ctcttacgtg
    35161 cgcctttctg ccggacgcga gcagatgaac gaacagactc aggcgatgtg ctttatggca
    35221 ggcgcaaact cgattttcta cggttgcaaa ctgctaacca cgccgaatcc ggaagaagat
    35281 aaagacctgc aactgtttcg caaactgggg ctaaatccgc agcaaactgc cgtgctggca
    35341 ggggataacg aacaacagca gcgtctggag caggcactga tgaccccgga cactgacgaa
    35401 tattacaacg cggcagcact atgagctggc aggagaaaat caacgcggcg ctcgatgcgc
    35461 ggcgtgctgc cgatgccctg cgtcgccgtt atccagtggc gcaaggagcc ggacgctggc
    35521 tggtggcgga tgatcgccag tatctgaact tttccagtaa cgattattta ggtttaagcc
    35581 atcatccgca aattatccgt gcctggcagc agggtgcgga gcaatttggc gtcggtagcg
    35641 gcggttccgg tcacgtcagc ggttatagcg ttgcgcatca ggcgctggaa gaagaactgg
    35701 ccgagtggct gggctattcg cgggcactgc tatttatctc tggttttgcc gctaatcagg
    35761 cggtcattgc cgcgatgatg gcaaaagagg accgcattgt cgctgaccgg cttagccatg
    35821 cctcattgct ggaagctgcc agtttaagcc cgtcgcagct tcgccgtttt gctcataacg
    35881 atgtcactca tttggcgcga ttgcttgctt ccccctgtcc ggggcagcaa ctggtggtga
    35941 cagaaggcgt gttcagcatg gacggcgata gtgcaccact ggcggaaatc cagcaggtaa
    36001 cgcaacagca caatggctgg ttgatggtcg atgatgccca cggcacgggc gttatcgggg
    36061 agcaggggcg cggcagctgc tggctgcaaa aggtaaaacc agaattgctg gtagtgactt
    36121 ttggcaaagg atttggcgtc agtggggctg cggtgctttg ctcaagtacg gtggcggatt
    36181 atctgctgca attcgcccgc caccttatct acagcaccag tatgccgccc gctcaggcgc
    36241 aggcattacg tgcgtcactg gcggtcattc gccgtgatga gggggatgca cggcgcgaaa
    36301 aactggtgtc actcattgcg cgttttcgtg ccggagtaca ggatttgccg tttacgctgg
    36361 ctgattcatg cagcgccatc cagccattga tcgtcggtga taacagccgt gcgttacaac
    36421 tggcagaaaa actgcgccag caaggctgct gggtcacggc gattcgcccg ccaaccgtac
    36481 ccgctggtac tgcgcgactg cgcttaacgc taaccgcagc gcatgaaatg caggatatcg
    36541 accgtctgct ggaggtgctg catggcaacg gttaataaac aagccattgc agcggcattt
    36601 ggtcgggcag ccgcactcta tgagcaacac gcagatctac agcgccagag tgctgacgtc
    36661 ttactggcaa tgctcccaca gcgtaaatac acccacgtac tggacgcggg ttgtggacct
    36721 ggctggatga gccgccactg gcgggaacgt cacgcgcagg tgacggcctt agatctctcg
    36781 ccgccaatgc ttgttcaggc acgccagaag gatgccgcag accattatct ggcgggagat
    36841 atcgaatccc tgccgttagc gactgcgacg ttcgatcttg catggagcaa tctcgcagtg
    36901 cagtggtgcg gtaatttatc cacggcactc cgcgagctgt atcgggtggt gcgccccgga
    36961 ggcgtggtgg cgtttaccac gctggtgcag ggatcgttac ccgaactgca tcaggcgtgg
    37021 caggcggtgg acgagcgtcc gcatgctaat cgctttttac cgccagatga aatcgaacag
    37081 tcgctgaacg gcgtgcatta tcaacatcat attcagccca tcacactatg gtttgatgat
    37141 gcgctcagtg ccatgcgttc gctgaaaggc atcggtgcta cgcatcttca tgaagggcgc
    37201 gacccgcgaa tattaacgcg ttcgcaattg cagcgattgc aactggcctg gccgcaacaa
    37261 caggggcgat atcctctgac gtatcatctt tttttgggag tgattgctcg tgagtaaacg
    37321 ttattttgtc accggaacgg ataccgaagt ggggaaaacc gtcgccagtt gtgcactttt
    37381 acaagccgcg aaggcagtag gttatcggac ggcaggttat aaaccggtcg cctctggcag
    37441 cgaaaagacc ccggaaggct tacgtaatag tgacgcgctg gcgttacagc acaacagcag
    37501 cctgcagctg gattacgcaa cggtaaatcc ttacaccttc gcagaaccca cctcgccgca
    37561 catcatcagc gcgcaagagg gcaggccgat agaatcattg gtaatgagcg ccggattacg
    37621 cgcgcttgaa caacaggctg actgggtgtt agtggaaggt gctggcggct ggtttacgcc
    37681 gctttctgac actttcactt ttgcagattg ggtaacacag gaacaactgc cggtgatact
    37741 ggtagttggt gtgaaactcg gctgtattaa tcacgcgatg ttgactgcgc aggcaataca
    37801 acacgccgga ctgactctgg cgggttgggt ggcgaacgat gttacgcctc cgggaaaacg
    37861 tcacgctgaa tatatgacca cgctcacccg catgattccc gcgccgctgc tgggagagat
    37921 cccctggctt gcagaaaatc cagaaaatgc ggcaaccgga aagtacataa accttgcctt
    37981 gttgtagcta tcctgtattt ggttaaattg cgagcgagat cgcgtcttcg attgactgca
    38041 atttaaccaa ttaaattcta aaataatcac gaaaaaaatt ttacttccgc ctcatgcggc
    38101 gaatgtggga attgcccagg cggcggggga taggggctgg agacagttat ccactattcc
    38161 tgtggataac catgtgta
//
LOCUS       DAZFQD010000033        38048 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.033, whole genome shotgun sequence.
ACCESSION   DAZFQD010000033 DAZFQD010000000
VERSION     DAZFQD010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 38048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38048
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.033"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(102..389)
                     /gene="yebO"
                     /locus_tag="ACHKTW_001754"
     CDS             complement(102..389)
                     /gene="yebO"
                     /locus_tag="ACHKTW_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HFO6462793.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTVESQKSVEDEDIIRLVAER"
     gene            complement(464..607)
                     /gene="mgrB"
                     /locus_tag="ACHKTW_001755"
     CDS             complement(464..607)
                     /gene="mgrB"
                     /locus_tag="ACHKTW_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HFO6462794.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            766..1005
                     /gene="yobH"
                     /locus_tag="ACHKTW_001756"
     CDS             766..1005
                     /gene="yobH"
                     /locus_tag="ACHKTW_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobH family protein"
                     /protein_id="HFO6462795.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLGITDCPLLRKSNIVVDNG"
     gene            complement(1148..1939)
                     /gene="kdgR"
                     /locus_tag="ACHKTW_001757"
     CDS             complement(1148..1939)
                     /gene="kdgR"
                     /locus_tag="ACHKTW_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HFO6462796.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDLDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLRCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            2116..3489
                     /gene="yebQ"
                     /locus_tag="ACHKTW_001758"
     CDS             2116..3489
                     /gene="yebQ"
                     /locus_tag="ACHKTW_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO6462797.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQMLTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIAAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMAAGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLMAAAILAVIAACVSGLRI
                     TQPRSRA"
     gene            complement(3535..4416)
                     /gene="htpX"
                     /locus_tag="ACHKTW_001759"
     CDS             complement(3535..4416)
                     /gene="htpX"
                     /locus_tag="ACHKTW_001759"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HFO6462798.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(4608..6656)
                     /gene="prc"
                     /locus_tag="ACHKTW_001760"
     CDS             complement(4608..6656)
                     /gene="prc"
                     /locus_tag="ACHKTW_001760"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HFO6462799.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNALWDSKVKFDELSLKLAGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FGPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLPKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            complement(6676..7374)
                     /gene="proQ"
                     /locus_tag="ACHKTW_001761"
     CDS             complement(6676..7374)
                     /gene="proQ"
                     /locus_tag="ACHKTW_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0033592 - RNA strand annealing activity
                     [Evidence IEA];
                     GO_function: GO:0034057 - RNA strand-exchange activity
                     [Evidence IEA];
                     GO_process: GO:0010608 - post-transcriptional regulation
                     of gene expression [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HFO6462800.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            complement(7471..7968)
                     /gene="msrC"
                     /locus_tag="ACHKTW_001762"
     CDS             complement(7471..7968)
                     /gene="msrC"
                     /locus_tag="ACHKTW_001762"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HFO6462801.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFGRFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            8098..9381
                     /gene="yebS"
                     /locus_tag="ACHKTW_001763"
     CDS             8098..9381
                     /gene="yebS"
                     /locus_tag="ACHKTW_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HFO6462802.1"
                     /translation="MALNTPQITPTKKITVRSIGEEQPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDTITGAMVFFCVIGAPLILVSSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERFY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGRCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSMLDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            9350..11983
                     /gene="yebT"
                     /locus_tag="ACHKTW_001764"
     CDS             9350..11983
                     /gene="yebT"
                     /locus_tag="ACHKTW_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
                     /protein_id="HFO6462803.1"
                     /translation="MSQETPASTTEAQIKNKRRISPFWLLPFIALMIAGWLIWDSYQD
                     RGNTVTIDFMSADGIVPGRTPVRYQGVEVGTVQDISLSDDLRKIEVKVSIKSDMKDAL
                     REETQFWLVTPKASLAGVSGLDALVGGNYIGMMPGKGKEQDHFVALDTQPKYRLDNGD
                     LMIHLQAPDLGSLNSGSLVYFRKIPVGKVYDYAINPNKQGVVIDVLIERRFTDLVKKG
                     SRFWNVSGVDANVSISGAKVKLESLAALVNGAIAFDSPEESKPAEAEDTFGLYEDLAH
                     SQRGVIIKLELPSGAGLTADSTPLMYQGLEVGQLTKLDLNPGGKVTGEMTVDPSVVTL
                     LRENTRIELRNPKLSLSDANLSALLTGKTFELVPGDGEPRKEFVVVPGEKALLHEPDV
                     LTLTLTAPESYGIDAGQPLILHGVQVGQVIDRKLTSKGVTFTVAIEPQHRELVKGDSK
                     FVVNSRVDVKVGLDGVEFLGASASEWINGGIRILPGDKGEMKASYPLYANLEKALENS
                     LSDLPTTTVSLSAETLPDVQAGSVVLYRKFEVGEVITVRPRANAFDIDLHIKPEYRNL
                     LTSNSVFWAEGGAKVQLNGSGLTVQASPLSRALKGAISFDNLSGASASQRKGDKRILY
                     ASETAARAVGGQITLHAFDAGKLAVGMPIRYLGIDIGQIQTLDLITARNEVQAKAVLY
                     PEYVQTFARGGTRFSVVTPQISAAGVEHLDTILQPYINVEPGRGNPRRDFELQEATIT
                     DSRYLDGLSIIVEAPEAGSLGIGTPVLFRGLEVGTVTGMTLGTLSDRVMIAMRISKRY
                     QHLVRNNSVFWLASGYSLDFGLTGGVVKTGTFNQFIRGGIAFATPPGTPLAPKAQEGK
                     HFLLQESEPKEWREWGTALPK"
     gene            12063..13502
                     /gene="rsmF"
                     /locus_tag="ACHKTW_001765"
     CDS             12063..13502
                     /gene="rsmF"
                     /locus_tag="ACHKTW_001765"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HFO6462804.1"
                     /translation="MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKISVADFLQLTAPYGWTLTPIPWCEEGFWIERDNEDALPLGSTAEHLSGLFYIQEA
                     SSMLPVAALFADDNAPQRVMDVAAAPGSKTTQIAARMNNEGAILANEFSASRVKVLHA
                     NISRCGISNVALTHFDGRVFGAAVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRPGGTLVYSTCTLNQEENEAVCLWLKETYPDAVEFLPLGD
                     LFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPAPKYKVGNFPFSPVKD
                     REAGQIRQAAAGVGLNWDENLRLWQRDKELWLFPVGIEALIGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIALASPDNMNAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPI
                     GLAKRIGSRLKNSYPRELVRDGKLFTGNA"
     gene            13620..13856
                     /gene="yebV"
                     /locus_tag="ACHKTW_001766"
     CDS             13620..13856
                     /gene="yebV"
                     /locus_tag="ACHKTW_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebV family protein"
                     /protein_id="HFO6462805.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            13961..14152
                     /gene="yebW"
                     /locus_tag="ACHKTW_001767"
     CDS             13961..14152
                     /gene="yebW"
                     /locus_tag="ACHKTW_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HFO6462806.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLYSMSDQRIRHGGCF
                     PIEDFIDGFWRPAQEYGDF"
     gene            complement(14153..14809)
                     /gene="pphA"
                     /locus_tag="ACHKTW_001768"
     CDS             complement(14153..14809)
                     /gene="pphA"
                     /locus_tag="ACHKTW_001768"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HFO6462807.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLISVGDVIDRGPQSLRCLQLLEQHWVCAVRGNHEQMAMDALASQQMSLWLMNGGD
                     WFIALADNQQKQAKTALEKCQHLPFILEVHSRTGKHVIAHADYPDDVYEWQKDVDLHQ
                     VLWSRSRLGERQKGQGITGADHFWFGHTPLRHRVDIGNLHYIDTGAVFGGELTLVQLQ
                     "
     gene            complement(15205..15546)
                     /gene="yebY"
                     /locus_tag="ACHKTW_001769"
     CDS             complement(15205..15546)
                     /gene="yebY"
                     /locus_tag="ACHKTW_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HFO6462808.1"
                     /translation="MMKKSILTFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTKAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(15559..16431)
                     /gene="copD"
                     /locus_tag="ACHKTW_001770"
     CDS             complement(15559..16431)
                     /gene="copD"
                     /locus_tag="ACHKTW_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
                     /protein_id="HFO6462809.1"
                     /translation="MLAFTWIALRFIHFTSLMLVFGFAMYGAWLAPLTIRRLLAKRFL
                     RLQQHAAVWSLISATAMLSVQGGLMGTGWADVFSPNIWQAVLQTQFGGVWLWQIVLAL
                     VTLIVALMQPRNVSRLLFMLTTAQFILLAGVGHATMNEGVTAKIHQTNHAIHLICAAA
                     WFGGLLPVLWCMQLIKGRWRHQAIQALMRFSWCGHFAVIGVLASGVLNALLITGFPPT
                     LTTYWGQLLLLKAILVMIMVVIALANRYVLVPRMRQDEDRAAPWFEWMTKLEWAIGAV
                     VLVIISLLATLEPF"
     gene            complement(16435..16809)
                     /gene="yobA"
                     /locus_tag="ACHKTW_001771"
     CDS             complement(16435..16809)
                     /gene="yobA"
                     /locus_tag="ACHKTW_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HFO6462810.1"
                     /translation="MTSTARSLRYALAILTTSLVTPSVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYI
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            16948..17178
                     /gene="holE"
                     /locus_tag="ACHKTW_001772"
     CDS             16948..17178
                     /gene="holE"
                     /locus_tag="ACHKTW_001772"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HFO6462811.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            17280..17936
                     /gene="yobB"
                     /locus_tag="ACHKTW_001773"
     CDS             17280..17936
                     /gene="yobB"
                     /locus_tag="ACHKTW_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein YobB"
                     /protein_id="HFO6462812.1"
                     /translation="MSFWKVAAAQYEPRKTSLTEQVAHHLEFVRAAARQQCQLLVFPS
                     LSLLGCDYSRRALPAPPDLSLLDPLCYAATTWRMTIIAGLPVEYNDRFIRGIAVFAPW
                     RKTPGIYHQSHGACLGRRSRTITVVDEQPQGMDMDPTCSLFTTGQCLGEPDLLASARR
                     LQFFSHQYSIAVLMANARGNSALWDEYGRLIVRADRGSLLLVGQRSSQGWQGDIIPLR
                     "
     gene            17960..18622
                     /gene="exoX"
                     /locus_tag="ACHKTW_001774"
     CDS             17960..18622
                     /gene="exoX"
                     /locus_tag="ACHKTW_001774"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HFO6462813.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSHLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGIKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERDPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(18619..20679)
                     /gene="ptrB"
                     /locus_tag="ACHKTW_001775"
     CDS             complement(18619..20679)
                     /gene="ptrB"
                     /locus_tag="ACHKTW_001775"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HFO6462814.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPEVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWETLLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASHDKLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPRENIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTGERRVLKQTEVPGFYAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDDHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     TPAD"
     gene            complement(20888..21547)
                     /gene="yebE"
                     /locus_tag="ACHKTW_001776"
     CDS             complement(20888..21547)
                     /gene="yebE"
                     /locus_tag="ACHKTW_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HFO6462815.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVPGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSMPLDERT
                     ERLILALVFAAKSDGHIDAKERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVRDGIERDLEQQKRTLA
                     E"
     gene            complement(21874..22230)
                     /gene="yebF"
                     /locus_tag="ACHKTW_001777"
     CDS             complement(21874..22230)
                     /gene="yebF"
                     /locus_tag="ACHKTW_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HFO6462816.1"
                     /translation="MKKRGAFLGLLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCKAGMAEYQRR"
     gene            complement(22297..22587)
                     /gene="yebG"
                     /locus_tag="ACHKTW_001778"
     CDS             complement(22297..22587)
                     /gene="yebG"
                     /locus_tag="ACHKTW_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
                     /protein_id="HFO6462817.1"
                     /translation="MAVEVKYVVIREGEEKMSFTSKKEADAYDKMLDTADLLDTWLTN
                     SPVQMEDEQREALSLWLAEQKDVLSTILKTGKLPSPQVVGAESEEEDASHAA"
     gene            22721..23899
                     /gene="purT"
                     /locus_tag="ACHKTW_001779"
     CDS             22721..23899
                     /gene="purT"
                     /locus_tag="ACHKTW_001779"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HFO6462818.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSESLFREAVAAIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWEYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRRLGVALATAESVVDAIERAKHAAGQVKVQG
                     "
     gene            complement(23955..24596)
                     /gene="kdgA"
                     /locus_tag="ACHKTW_001780"
                     /gene_synonym="eda"
     CDS             complement(23955..24596)
                     /gene="kdgA"
                     /locus_tag="ACHKTW_001780"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFO6462819.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(24633..26444)
                     /gene="edd"
                     /locus_tag="ACHKTW_001781"
     CDS             complement(24633..26444)
                     /gene="edd"
                     /locus_tag="ACHKTW_001781"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004456 - phosphogluconate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HFO6462820.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYVEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEL
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PRIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            complement(26679..28154)
                     /gene="zwf"
                     /locus_tag="ACHKTW_001782"
     CDS             complement(26679..28154)
                     /gene="zwf"
                     /locus_tag="ACHKTW_001782"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFO6462821.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKAGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFSRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            28492..29361
                     /gene="hexR"
                     /locus_tag="ACHKTW_001783"
                     /gene_synonym="yebK"
     CDS             28492..29361
                     /gene="hexR"
                     /locus_tag="ACHKTW_001783"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HFO6462822.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAAMALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANSTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            29489..30931
                     /gene="pyk"
                     /locus_tag="ACHKTW_001784"
     CDS             29489..30931
                     /gene="pyk"
                     /locus_tag="ACHKTW_001784"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFO6462823.1"
                     /translation="MSRRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLKVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(31062..32033)
                     /gene="lpxM"
                     /locus_tag="ACHKTW_001785"
                     /gene_synonym="msbB"
     CDS             complement(31062..32033)
                     /gene="lpxM"
                     /locus_tag="ACHKTW_001785"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416369.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HFO6462824.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVDWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IARRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(32153..33475)
                     /gene="mepM"
                     /locus_tag="ACHKTW_001786"
     CDS             complement(32153..33475)
                     /gene="mepM"
                     /locus_tag="ACHKTW_001786"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HFO6462825.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            complement(33491..34423)
                     /gene="znuA"
                     /locus_tag="ACHKTW_001787"
     CDS             complement(33491..34423)
                     /gene="znuA"
                     /locus_tag="ACHKTW_001787"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HFO6462826.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPEAKQV
                     TIAQLEDVKPLLMKSIHGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            34502..35257
                     /gene="znuC"
                     /locus_tag="ACHKTW_001788"
     CDS             34502..35257
                     /gene="znuC"
                     /locus_tag="ACHKTW_001788"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015633 - ABC-type zinc transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HFO6462827.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNRPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            35254..36039
                     /gene="znuB"
                     /locus_tag="ACHKTW_001789"
     CDS             35254..36039
                     /gene="znuB"
                     /locus_tag="ACHKTW_001789"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HFO6462828.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLIAIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQRVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFILSMMKKQAS"
     gene            complement(36186..37196)
                     /gene="ruvB"
                     /locus_tag="ACHKTW_001790"
     CDS             complement(36186..37196)
                     /gene="ruvB"
                     /locus_tag="ACHKTW_001790"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFO6462829.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(37205..37816)
                     /gene="ruvA"
                     /locus_tag="ACHKTW_001791"
     CDS             complement(37205..37816)
                     /gene="ruvA"
                     /locus_tag="ACHKTW_001791"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFO6462830.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDASSETLIREALRAAL"
     gene            complement(37955..38020)
                     /gene="yobI"
                     /locus_tag="ACHKTW_001792"
     CDS             complement(37955..38020)
                     /gene="yobI"
                     /locus_tag="ACHKTW_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response small protein YobI"
                     /protein_id="HFO6462831.1"
                     /translation="MYIFITHFFTEYVIFKYLLPI"
BASE COUNT         9142 a         9811 c         9371 g         9724 t
ORIGIN      
        1 accagagttt taatttgaag cagttgcacc atggcagtgc gtataatcag gatgaattga
       61 gtaacccttc aacgacgggg ttactcaaga ttgaggaagg attatcgttc ggcgaccaga
      121 cgaataatat cttcgtcttc aaccgatttt tgactctcaa cggtcttttt atctgctttc
      181 tcaggttcgt ttgcttcgca caaacgacgt aacagtgcat tttgacgttt ttgctgatcc
      241 agcaacgcct caagcagttc aatctgttcg ttagtccgag aactggcacg attgataaaa
      301 aaccacaaga tgagcccgat aagaagaacc accaccgata caaccaaaga cgcaatgttc
      361 atcacgcctg aatttacaac ttcgttcatt tcaccacctc tgagtagagg cgctattcta
      421 ccactgctgg agaggaagaa aatctagtgc tgaaaaaatg atatcaccac gggataaact
      481 ggttaatggc acaaattccg ctgaaaaatt gtacatcctg atcgcacatc atgttgaata
      541 cctgcgccca aagcagcaag caagccaaca ccacgacaac cagaacgacc catcgaaact
      601 ttttcactcc aatctccgct tcacctacct tatgtcatta aactagcatg cgttagttaa
      661 acagcgccta actatgtcga tattcgtgct ttttcggaat gagtagcttg cttcagcgca
      721 tattgccgct acgattaagc gaacataaaa agagaagagg ttgtaatgcg attcatcatt
      781 cgcacagtta tgctgatagc actggtatgg ataggattat tactcagcgg atatggcgtt
      841 ttgattggaa gcaaagagaa tgccgcagga ttaggtttgc aatgtaccta tctgactgcc
      901 agaggcacca gcacggtaca atacttgcac actaaaagtg gtttcttggg gataacagat
      961 tgcccactgt tacgtaaaag caatatcgtg gtcgataatg gttaagggta ataaataacc
     1021 agattctcac tcatgagtaa aatattctta tagcaagcgc gtttaatata acgcaccttc
     1081 aactttattt ttctcatgct aaataaagaa cctgtctgtt tgatattccg acaggttctt
     1141 actctcatca gaacggatag tcgtgataac ccatttgggc agaaattttg cgcgctgcgg
     1201 tatgcaacat tgcgacatat tcctgtaaac gctcttcaga gaaacgcaac gtcgggaagg
     1261 agatgctcaa accggcaatg accacgccaa agcgatcaaa taccggtacc gcaatgcatc
     1321 gcagcccttc ttcctgctct tcgttatctt cgccataccc ctgctcgcgc acctggtcca
     1381 gaacgggtaa taacgcttct gtactggtga tggtccgctc ggtactgcgt ttatactcca
     1441 cgccctcaag aatttgcttc acttcatcga gatcgcgcca tgccagcagt accttaccaa
     1501 tcgcggtgct gtacagcgga ttacgacgcc caatccgtga atacatgcgc aaattgtaca
     1561 tagagtcaat tttgtgaatg taaacaatac tgtcttcgtc cagtgcgccg aggtggatag
     1621 tttctttggt caggcgggag atctcacgca tctggatatc tgcgctacga attaaatcga
     1681 cgttttgtaa cgcgcgagcg cccagttcaa acaatttcag ggtcagggaa tatttctccg
     1741 actccccttc ctgcgccaca taacctaagg ttttcatggt ctgtaaaaag cgataaacgg
     1801 tgctttttga catcatgacg cgctgcgaca gctcggttat ccctatttcg cgctcttcac
     1861 ccagcgcctg caaaatgcca aaaactttta gcacggaaga tacagaatca ggctgtttat
     1921 ccagatctgc gttagccatt tatcacctca ttgcgagtgt tttataaaaa tcagaactgt
     1981 tttttattat aatttcgcac cagggtggtc gcaatccatc ttttgccggt tagttacaat
     2041 tctgcgacat ccaccgtgaa tatcagtgct agaatcatac ccctgttgat tattcaccaa
     2101 agatataaaa ttcctatgcc aaaagttcag gccgacggcc tgccattgcc ccagcgatac
     2161 ggtgcgatat taaccattgt gattggtatt tcgatggctg tccttgacgg cgcaatcgcc
     2221 aacgtcgccc tgccaacaat cgccacggac cttcatgcca cgccagccag ttccatctgg
     2281 gtagtgaacg cctatcaaat cgccattgtc atctccctgc tctcgttttc gtttctgggc
     2341 gatatgtttg gctatcgacg tatttataaa tgcggtctgg tcgtttttct gttgtcttca
     2401 ctgttctgcg ccctttctga ttcgctgcaa atgctcaccc ttgcgcgtgt catacaaggt
     2461 ttcggcggtg cagcgttgat gagcgttaat accgcactta tccgcctgat ctatccacaa
     2521 cgttttctgg ggagagggat gggcataaac tcgtttattg ttgccgtctc ttctgctgcc
     2581 gggccgacaa ttgctgcagc aatcctctcc atcgcatcct ggaaatggtt atttttaatc
     2641 aacgtaccgt taggtattat cgccctgctt ctggcgatgc gttttctgcc acccaatggt
     2701 tctcgcgcca gtaaaccccg tttcgacctg cccagcgccg tgatgaacgc gttaaccttc
     2761 ggcctgctta tcactgcgtt gagtggtttc gctcaggggc aatcgctgac gttaattgct
     2821 gcggaactgg tggtaatggt tgttgttggt attttcttta ttcgccgcca gctttctctt
     2881 cccgtaccgc tgctaccggt ggatttactg cgtatcccgc tgttttcact ttctatttgc
     2941 acatctgttt gctctttctg cgcacaaatg ctggcaatgg tttccctgcc cttttacctg
     3001 caaaccgtgc tcgggcgtag tgaagtcgaa acaggtttac ttctgacacc gtggccgtta
     3061 gcaacgatgg tgatggctcc gttggcaggc tatttgattg aacgcgtaca tgcaggattg
     3121 ctgggtgctc tagggttgtt catcatggct gcggggcttt tttccctggt tctgctgccc
     3181 gcgtcacctg cggatatcaa tattatctgg ccgatgatct tatgtggcgc tggatttggc
     3241 ttgttccagt cacccaataa ccacaccatt attacctccg ctcctcgcga acgtagcggt
     3301 ggagccagtg gcatgttagg aacggctcgt ctactgggtc agagtagcgg cgcggcactg
     3361 gtggcgctga tgctaaatca gttcggtgat aatggtacgc acgtttcgct gatggctgcg
     3421 gctattctgg cagtgattgc tgcctgtgtc agtggtttac gtatcactca gccacgatcc
     3481 agggcataaa aaaagcgcgt cgatcaggac gcgcttttta gtatttactt catattactt
     3541 caggtattca cccgtacgca gagcttcaat tcgtttatcc agcggcgggt gagtcatgaa
     3601 caactcactg agcgatttcg acttaccgtt aatgcagaaa gccatcatgc tggttgcttc
     3661 ttgcggttca tagctggttt tcaggcgctg cagcgcggca atcattttct cgcgaccaac
     3721 cagttttgcc gaaccggcat cagcatggaa ttcacgatga cgcgagaacc acatggtgat
     3781 aatgctcgcc agaataccaa acaccagttc cagaaccgtt gcaaccgcaa agtagatcag
     3841 cgggttgccg ttgctctctt caccttcatc acgatttccg cccataaaac ccgcggcaag
     3901 ctgcgccaga atacgggaaa taaagataac gaaggtgttc accacgccct gaatcagcgt
     3961 catggtgacc atatcaccat tggcgatgtg gctgatttcg tgagcaatta ccgcctcggc
     4021 ttcatccggg ctcatgttct gcagcaaacc ggtgctgaca gcaaccagag aggcatcacg
     4081 gcgcgcaccg gttgcaaaag cgttgatgtc cggcgcatgg tagatagcca cttgcggcat
     4141 agcgatccct gcctgacgag cctgggttgc tacagtatta accagccaac gttccctttc
     4201 gttacgcggt tgctcgatca cttccccgcc aacagatcgt aatgccatcc atttggacat
     4261 cagaagcgaa acgaaggaac caccaaaacc gaacagcaag gccatgatca tcagcccctg
     4321 aacgctgctc gactgtatcc ctgtcaggct cagtaccagc ccgaaaacga ccattacggc
     4381 caggttcgtt agcaggaaga gcgcgattcg catcataatt ttctttttac ctcagtttaa
     4441 caaaacgcaa tatgcgatac ccacatcgta tgggttacgc gactattttc aagtctggat
     4501 agtgcgtaag tcaccagaaa gacacaactt tacattttgt agcatctgat ttacggcatc
     4561 ttgccgctgt taaaaaatca ggcacaattt cttgtgcctg attgatatta cttgacggga
     4621 gcgggttgtt ccgcgggtct ggctttttca agcttcgcca gatcgagtgc gatattcacc
     4681 gtctcatcca gataaggatc cggctcctgg taatctttcg gtagatcatc cagcttcttc
     4741 aactccggtt taccttcgcg tttaaagcgt tcgttcaaac gcgccagacg cgtcgcatca
     4801 tcttcattat tctctttctc acgcacagcg tagttcagag aaacgatatt gcgcttgtcc
     4861 ttcatagcgt tgaagcgcgc gatatccttc atgatgttct ggaactcagg atctttcgcg
     4921 atacgcgcat tatgttcctt cagcagctcc ggcccaaagg ccgttaaatc gcctgatttc
     4981 acataagtcg cggcatcaat gctatcccac ggcagcgcgt tatcttcgaa tttctcaccc
     5041 gtttccgttt cttcattacc cgtcggcatg atgatgtctg gcgttacgcc tttacgttgc
     5101 gtactgccgc cgttaacgcg atagaatttc tggatcgtgt actgcacaga acccagcgct
     5161 ggccattcag gacgtaacat ctgatcgtaa atacggttca atgaacggta ttgctgaacg
     5221 gtgcctttac caaacgtcgg ttcacccaca accagcgcac gaccgtaatc ctgcattgcc
     5281 gcggcaaaga tttctgaagc cgaagcactg aaacggtcaa ccagtaccac cagcgggcct
     5341 ttatagaaca cctgcccgtc ggtatcgctg tcttcacgaa ccttgccgtt gttatcgcgg
     5401 acctgaacaa tgggacccgc aggaataaac agacctgaga gcgataccgc ttcggtcaac
     5461 gccccaccgc cattgctacg caggtcgatg atcacactgc tgacattctg tttttccagt
     5521 ttctgcagtt gaactttgac atcgtctgtc aaacccacat agaagcccgg aatatccagc
     5581 acgccgactt tctctttacc gacggtcttc accgacattt taaccgcgcg gtcttcgaga
     5641 cgaatacgtt cacgggtcaa tgttacagtg cgggtcttgg tccctttacc agcaggtaaa
     5701 atttccagac gaactttact gcccttcggc cctttaatta aggcaaccac atcatcaaga
     5761 cgccagccaa tcacgtcaac catcggcttg cctgtttgac caacaccgac aattttgtca
     5821 ccaacgctga tagctttact cttcgctgcc ggaccacctg ccaccatcga attgataacg
     5881 gtgtagtcat catccatttg cagcactgcg ccaatacctt ccagcgacaa actcatttca
     5941 gtgttgaact gttcggtatt acgcggggaa agatagttgg tatgcgggtc gatttcacgc
     6001 gcaaacgccg tcattgccag cgagaaaaca tcttcgctgt tggtttgcgc cagacgacga
     6061 atggcaaatt tgtagcggcg tgtcagggtt tcacgaattt ctttatccgt ttttcctgcc
     6121 agcttcaggc ttaactcgtc gaatttgact ttactgtccc acagcgcgtt caactcagcc
     6181 tcgtttttcg gccagggcgc tttgctgcgg tcaaggttat aagtgtcgtt gccggtgaaa
     6241 tccatcggct tttccagtac cgacaaagcg tactggtaac gctcaaaacg gcgcttttgc
     6301 gccagattgt agagatcgta gaaaacgtcg agtttgcctg aacgcagttc atcgcctaac
     6361 tcggttttct ttttcgcgaa ctgttcaaca tcgcttgcca gcagcacgtt gtggctgtaa
     6421 tcgagcagat tcaggtagcg gtcaaagatt ttggccgaaa atgcctgatc gaggtcgaac
     6481 tggcgataat gagaacgggt gaagcgcgac gttacgcgct cacttaccgt cgcatgctgc
     6541 gtctcttcct ttaataccgg aatttgatca gcacgcgtga tatcttctac agcgaaggtc
     6601 tggcctgcta ttgcaagcag gccagctaac gcggtaagcc taaaaaacat gttcatgcct
     6661 ggcccggcct ccgtttcaga acaccaggtg ttctgcgcgc acaatcaaag acatacccga
     6721 attcagctgg acgcggacgc cgtctttggt gatttctaat acggtggcat ccatcgcgtt
     6781 ttgacccgct ttcaccttca gggcttgtcc gacagtcaga gctgaaatgt cagaaaccgg
     6841 ggtgtgctgt tcttcgcgag gtgcttttac tgtttttggc gctttctcta ccggcttttg
     6901 cgcacgaggt ttacgttcag cgccttcttt gcggcgtggc gtggtcggac gtggcttgcg
     6961 ttcgcggcgc ggtgcgtctt ctttctcacc agcagctgcg gcagcttcgc gttttttcgc
     7021 ttgctgttca gcacgctgtg cctgaacacg cgctttcgct tcttcaagct gcttgcgagc
     7081 atgctctaca tgttgctcgt ccagctcacc gcatgggttg ccgtcaagat cgacacgcgt
     7141 tgcgccgggt ttaacaccgt aaagataacg ccagctcgaa gtgtagagac gtaaagcgga
     7201 tcgtaattgc gttttgctca ggttcatttc cccggcaaca cgatcgacca aatcctgaaa
     7261 aataccgatt ttcagcggac gcgcttcacc ttccgcactg aaacagtggg gaaaacgttc
     7321 ggccagaaac gcgattactt ctttactgct attcaactta ggttgatttt ccatgaaatt
     7381 tcctgattac aacggacgta gccaacaagc gcaggcatga acaggcgtca ttataatgac
     7441 gctaccagta aatgctacgt tatccgttga ttatcctgcg acgctcgcaa agaatttttt
     7501 gtaatccgtc gttgcaagca ctttttcaag ctgtgccaca agctgacgta agccttgctc
     7561 gtcctcgtct gtaaagcgac cgaagacggt actatcgatg tcgagaacac caataatctg
     7621 atttttcacc accagcggca gaacaatttc agaattactc gccgcatcac aggcaatatg
     7681 cccgtcaaac gcgtgaacat cctcgatacg ctgcacttga ttgcgggcaa ccgcagtgcc
     7741 gcacaccccg cgcccgacgg gtatccggac acaggcaatt ttgccctgaa atggtccgag
     7801 taccagtgta tcgtcctcaa gcaaataaaa acctgcccag tttacgtcag tgagacgctc
     7861 gtataacaac gcactggtgt tcgcaagcgt tgccagaaaa ctggtttctc ctgccatcag
     7921 cgcgttaaag tcgcgattta aatccgcgta aaattctgtt ttgttcatta tataatcact
     7981 tggttgtctt acctggaact gccagcctat taaaataagc attaaatgcg ttaatgctca
     8041 agatcattcc catcatgggt taatattaat gttaattctt attacatttg gcacgtcatg
     8101 gctcttaaca caccacaaat tacgccgaca aaaaagataa cagtgaggtc aatcggcgag
     8161 gaacagccgc gtggtgatta ccaacgttgc ccgcaatgcg acatgctgtt tagcctgccc
     8221 gagataaatt ctcatcagag tgcctattgt ccgcgctgtc aggcaaaaat tcgcgacggg
     8281 cgcgactggt cgctaacgcg cctggcggca atggctttca ccatgctgtt gctgatgccg
     8341 tttgcctggg gcgaaccgct gttgcatatc tggctgttag gtattcgtat cgacgccaac
     8401 gtcatgcaag gcatctggca aatgaccaaa cagggcgata cgataacggg ggcgatggtc
     8461 tttttctgcg ttattggtgc ccccctcatt ctggtgtcct ccatagcata tttatggttt
     8521 ggtaaccgac tgggaatgaa tttacgtccg gtactgctga tgcttgagcg acttaaagag
     8581 tgggtaatgc tggatatcta cctggtcggc attggcgttg cttctataaa ggtacaggat
     8641 tatgcccata tccaggcggg tgtcggcttg ttctcttttg tggcgttggt gattttaacg
     8701 acggtgacgt tgtcacatct taatgtcgag gagctgtggg agcgatttta tccgcagcgc
     8761 cccgctacgc gtagggacga gaaacttcgt gtctgtcttg ggtgccattt taccggctat
     8821 cctgatcagc gtggtcgctg cccgcgttgc catatcccgc tacgcctgcg tcgccgtcat
     8881 agtttgcaaa aatgctgggc ggcgctgtta gcgtcaatcg ttttgttgtt acctgccaac
     8941 ctgttgccta tttctatcat ttatctgaac ggaggacggc aggaagatac aattctttcc
     9001 ggaattatgt cgctggcaag tagcaacatt gcggttgcgg gaatcgtgtt tatcgccagt
     9061 attctggtac cgtttactaa agtgatcgtc atgttcactt tactgttgag cattcatttt
     9121 aaatgccagc aaggtttacg cacacgcatt ctgttactgc ggatggtgac ctggattggt
     9181 cgctggtcga tgctcgacct gtttgtcata tctttaacca tgtcgctgat taatcgcgat
     9241 cagatcctcg cttttactat gggaccggct gcgttttatt tcggcgcagc ggtaattttg
     9301 actattcttg ctgtggaatg gctggacagc cgcttacttt gggatgcaca tgagtcagga
     9361 aacgcccgct tcgacgactg aagcgcagat taaaaataaa cgccgtatct cacctttctg
     9421 gctgctgccg ttcatcgcgc taatgattgc cggttggctg atttgggaca gttatcagga
     9481 ccggggtaat accgtcacca tcgactttat gtcggcggat ggtattgtcc cgggccgtac
     9541 gcctgttcgt tatcagggcg ttgaagtcgg aacagtgcag gatatcagcc tcagcgacga
     9601 tcttcgtaag attgaagtca aggtcagcat caagtccgat atgaaagatg cgctgcgcga
     9661 agagactcaa ttctggctgg tgacgccaaa agcatcgttg gcaggtgtct ccgggctgga
     9721 cgccctcgtc ggtgggaact atatcggcat gatgccgggt aaaggtaaag agcaggatca
     9781 ctttgtcgca ctcgataccc aaccgaaata tcggctggac aatggcgatc tgatgatcca
     9841 cctgcaagcc cccgatctcg gttcgctgaa cagcggttca ttggtctatt tccgcaagat
     9901 cccggtggga aaagtctacg actatgccat caatcccaac aaacaaggcg tggtgattga
     9961 tgtcctgatc gagcggcgtt ttaccgatct ggtgaaaaaa ggtagccgtt tctggaacgt
    10021 ttccggcgtt gatgccaatg tcagtatcag tggcgcgaag gtgaaactgg aaagcctggc
    10081 ggcactggtt aacggtgcga ttgccttcga ttcaccagaa gagtcgaaac ctgccgaggc
    10141 ggaagatacc tttggtctgt atgaagacct cgcccatagc cagcgtggcg taataataaa
    10201 actggaactg ccgagtgggg ccggattaac cgccgactcg acgccattaa tgtatcaggg
    10261 actggaagtc ggacagctaa ctaaactgga tttaaatcct ggtggtaaag tcactgggga
    10321 aatgacagtt gatcccagcg tcgttaccct gcttcgtgaa aatacccgca tcgaattacg
    10381 taatccgaaa ttatccctta gcgatgccaa tctcagcgcc ctgctgactg gcaaaacctt
    10441 cgagttggta cccggcgatg gcgagccacg caaagagttc gttgttgtgc caggcgaaaa
    10501 agcactgctg catgaacctg atgttctgac gctgaccctg accgcgccgg aaagttacgg
    10561 tattgatgcg ggtcagccgc tcattcttca cggcgtgcag gtaggccagg ttatcgatcg
    10621 taaactcacc agcaaaggcg tcacctttac cgtcgccatc gagcctcagc atcgagaact
    10681 ggtaaaaggc gatagcaaat ttgtcgtcaa cagccgtgtc gacgtgaagg tggggctgga
    10741 tggcgttgag tttctcggtg ccagcgcctc agaatggatt aacggcggga tacgtattct
    10801 gccgggcgat aaaggcgaga tgaaagccag ctatccactg tatgccaatc tggaaaaagc
    10861 gctggagaac agccttagcg atttacccac cacaaccgtg agtttgagtg cagagacgct
    10921 gccggatgtg caggcaggat cggtagtgct ctaccgtaaa tttgaagttg gtgaagttat
    10981 taccgtccgt ccgcgagcta acgcgtttga tatcgatctg catattaagc cggagtatcg
    11041 caaccttctg accagcaata gcgtgttctg ggcagaaggc ggggcgaaag ttcagctgaa
    11101 tggtagtggc ctgaccgtac aggcatcccc gctctccaga gcattaaagg gagccattag
    11161 cttcgataac ctcagcggtg ccagcgccag tcagcgtaaa ggcgacaaac gaattctgta
    11221 tgcttccgaa acagcggccc gtgcggttgg tgggcagatt acgcttcacg ctttcgatgc
    11281 cggaaaactg gcggtcggga tgccaattcg ctatctcggt attgatatcg ggcaaatcca
    11341 gacgctggat ctgattaccg cgcgcaatga agtacaggca aaggcggtgc tctatccgga
    11401 gtatgtccag accttcgccc gcggcggtac gcgcttctcg gtggtcacac cgcaaatttc
    11461 agccgcgggc gttgagcatc tagatactat cctccagccg tatatcaacg tcgaaccagg
    11521 ccggggcaat cctcgccgcg actttgaatt acaagaggcc accattactg attcgcgtta
    11581 cctggatggc ttaagcatta ttgttgaagc gccggaagcc ggttcgttag gtattggtac
    11641 gcctgtgctg ttccgtggtc tggaagtcgg tacggttaca ggaatgacgc tggggacatt
    11701 gtcagatcgc gtgatgattg cgatgcgcat cagtaaacgc tatcaacacc tggtgcgtaa
    11761 caattccgtc ttctggttgg catcgggtta cagtctggac tttggtctga cgggcggcgt
    11821 agtgaaaacc ggcaccttta accagtttat ccgtggcggc atcgccttcg ccacgcctcc
    11881 gggtacgcca ctggcaccga aagcccagga aggcaaacac ttcctgttgc aggaaagtga
    11941 accgaaagag tggcgtgaat ggggaactgc gcttcccaaa taatgcccac tgctccggcg
    12001 tgcctgcgcc ggagcgttta tgctaaactg cgcgcctgtt tttttgccag tggtacatgc
    12061 tcgtggccca acacaccgtt tatttcccgg acgcctttct gacacaaatg cgcgaagcga
    12121 tgccttcgac gctctcattt gatgattttc ttgccgcctg tcagcgcccg ttgcgccgca
    12181 gcattcgcgt taatacgctg aaaatctccg ttgctgattt cctgcaatta accgctcctt
    12241 atggctggac gcttacgcca attccgtggt gtgaagaagg tttctggatt gaacgcgaca
    12301 atgaagatgc attgccattg ggtagtaccg ccgagcattt aagcggcctg ttttatattc
    12361 aggaagccag ttcaatgttg cccgttgccg ccttgtttgc tgacgataat gcaccacagc
    12421 gggtgatgga tgtcgcagct gcgccaggct ccaaaacgac gcaaattgcc gcgcggatga
    12481 ataacgaagg ggcaatcctt gccaatgagt tttccgccag tcgggtaaaa gtgttacatg
    12541 ccaatatcag ccgctgtggc atcagtaatg ttgcgctcac acattttgat ggccgcgtgt
    12601 ttggtgcggc agtgccagaa atgttcgatg ccattttgct ggacgctccc tgctctggcg
    12661 aaggcgtggt gcgtaaagat cccgatgcgc taaaaaactg gtcaccagaa agcaatcagg
    12721 aaatcgcagc tacacaacgg gagcttatcg acagcgcctt tcatgcatta cgtcctggtg
    12781 gtacgctggt ttactcgacc tgtaccttaa accaggaaga aaacgaagcc gtttgcctgt
    12841 ggctgaaaga gacttacccc gacgcagtag agtttttacc acttggcgat ctcttccctg
    12901 gtgcaaacaa agcgctgacc gaagaaggct ttttgcatgt tttcccacaa atttacgact
    12961 gcgaaggctt cttcgttgct cgtctgcgta aaactcaggc gattcccgcc ttacccgccc
    13021 ccaaatacaa agtgggcaat ttcccgttca gcccggtgaa agatcgcgaa gctggacaaa
    13081 ttcgtcaggc ggctgcaggt gttggcttaa actgggatga aaacctgcgc ctctggcagc
    13141 gtgacaaaga actgtggttg ttcccggtgg gcattgaagc cctgatcggt aaagtccgat
    13201 tttctcggtt ggggattaaa cttgccgaga cgcacaacaa aggttatcgc tggcagcatg
    13261 aagcggttat tgctcttgcc tcacccgata atatgaatgc attcgagctg acaccgcagg
    13321 aagcggagga gtggtatcgc gggcgcgatg tttacccgca agccgcgcca gtggcggatg
    13381 atgtattggt tactttccag catcaaccga ttggtttagc caaacggatt ggttcgcgat
    13441 tgaaaaacag ctatccgcgt gaactggtgc gcgatgggaa actttttacc ggtaacgcct
    13501 gacagcgcac aaaaaaagcg cactttttga ctggcacatt cggctgcctc aactaggctg
    13561 aaaaatggtg cgatcggact ggtcgtacca caatcggcag ctaaatggag agcacgaaga
    13621 tgaaaaccag tgtgcgcata ggcgcttttg aaatcgacga cggcgaatta cacggtgaat
    13681 cgccaggtga tcgaacgtta accattcctt gtaaatctga ccccgattta tgtatgcaac
    13741 tggatgcctg ggatgctgaa accagtatcc ctgccctgct aaatggcgaa cactctgtcc
    13801 tttaccgtac ccgttacgat caacaatctg atgcctggat tatgcgtctt gcctgatcca
    13861 aaaagaaccc gtcggcatgg cgggttattt gtcctggtta ttcccccgtt gtaaaatctc
    13921 tcctaaactt aacggtacgg caccacactt cggggatgaa atgttcgcgc tggtactttt
    13981 tgtttgctac ctggatggcg gttgtgaaga tattgttgtg gatgtctaca acacggaaca
    14041 gcagtgtctt tattctatga gcgatcaacg gatccgccat ggcggttgtt ttccgattga
    14101 ggattttata gatggtttct ggcgaccagc acaggagtat ggtgattttt aattattgca
    14161 attgcacaag agtcagttcg cccccaaaga cagcaccggt atcaatataa tgcaggttgc
    14221 caatatccac gcgatgtcgc aacggtgtat gaccaaacca gaaatgatca gcacctgtaa
    14281 ttccctgccc tttttggcgt tcacctaatc gcgagcggct ccacaagacc tgatgcaaat
    14341 caacgtcctt ttgccattca taaacatcat ctggataatc ggcatgagca ataacatgtt
    14401 tgccggtacg actgtgtact tcaagaataa agggcaaatg ctgacatttt tccagcgccg
    14461 ttttcgcttg tttctgttga ttatctgcca gcgcaataaa ccagtcgccg ccattcatca
    14521 accacaaaga catctgctgg gatgccagcg catccatcgc catctgttca tgattgcctc
    14581 ttaccgcaca aacccaatgt tgttccagta actgcagaca acgtaaactt tgcggcccac
    14641 gatcgataac gtctcctact gagataagta aatctcgcca cggatcaaaa cgacaatgcc
    14701 ataatttgcg gcgcaactgc tcaagacaac cgtgtatatc gccagaaagc cagatatgtc
    14761 gccattgatg acccgcaatt ctctgataaa cgggcgcagg ctgtttcatc aatattttcc
    14821 tcccgcgcta aagatcacat aatcttaaca agaatgttaa aaaacgctgg actcagacag
    14881 tagagtgtgt gttatggttg actataaagt cagcgaagga aatgcttctg gcttttaaca
    14941 gataaaaaga gaccgaacac gattcctgta ttcggtccag ggaaatggct cttgggagag
    15001 agccgtgcgc taaaagttgg cattaatgca ggcttagttg ccttgccctt taagaataga
    15061 tgacgacgcc aggttttcca gtttgcgtgc aaaatggtca ataaaaagcg tggtggtcat
    15121 cagctgaaat gttaaaaacc gcccgttctg gtgaaagaac tgaggcggtt tttttattgg
    15181 aaatcaaaag gctattttag gtaattaaca gagtttttca gctcgttcta taaacggtgc
    15241 cagactcatt ttttcgccgg gattgttagg atcatcaatc tgaatcaccg aaatgggttg
    15301 ggctttagtc ttcccactgg caacttcctt ttgtgcgata tcgtttaaag gatactgcac
    15361 gagggtactt gggttaatga catacaaagc attacccggt ctgcaagtca gcatcacctc
    15421 ttcgcgatta aacgcccatt tgtctttacc cacttcaaaa cgactgacgg taatcacctg
    15481 cggtgcagcc agcgccgctg cagaactggt gagtaacaga aacgtcagaa tacttttttt
    15541 catcattttc gtaatccatc aaaaaggttc gagagtcgca agcaggctga tgatcaccag
    15601 cactaccgca cctatcgccc attcgagttt agtcatccac tcaaaccagg gtgccgcacg
    15661 atcttcatct tgcctcatgc gcggtacaag aacataacga ttagccagcg caatgaccac
    15721 cataatcatc accaggatgg ctttgagcaa taagagttgg ccccagtaag tcgtaagcgt
    15781 ggggggaaat ccggtaatca acaatgcatt aagcacgccg cttgccagta cgccgatcac
    15841 cgcaaaatgc ccgcaccagg aaaaacgcat cagcgcctga atggcctggt gtcgccaacg
    15901 gcctttgatg agttgcatac accagagcac cggcagtaat cccccaaacc acgccgccgc
    15961 acaaatcagg tgaatcgcgt gattagtctg atggattttc gccgttaccc cttcattcat
    16021 cgtcgcatgc cccactcccg ccagcagaat aaattgcgcg gtagtgagca taaacagcaa
    16081 acgtgacaca ttccgcggtt gcataagggc gacgatcaac gttacgaggg cgagaacaat
    16141 ttgccataac cagacgccac caaactgcgt ttgcaaaaca gcctgccaaa tgtttggcga
    16201 aaatacatct gcccatcccg ttcccatcaa cccgccctga acggagagca ttgcagtggc
    16261 actgatcaaa ctccagacgg cagcgtgctg ttgtaaacgc agaaaacgct tcgccagcaa
    16321 gcgacgaatc gttaaggggg ccagccaggc accgtacatt gcgaagccaa agaccagcat
    16381 cagcgaggtg aaatggataa atcgcagcgc gatccaggta aacgccagca tgatttattt
    16441 cacgctaaag gtgtagtgtc ctttcgtttt atgcccgtcc accgaaacaa catgccagtc
    16501 aacgatgtag gttccgggtt tcagcgaatc ggccagtgga acaatcagtt gcttttggtc
    16561 ctgctcattt cgcttcgccg gtaatgtttt aatattttcg ttttttggcc ccgtgatttt
    16621 tgcaccactg aatcctgttt caacaccttc cgagaagttt aaagtgattg cctgcggtgc
    16681 agctgtcact tgcgcgtttg ccgcaggata ctgatgcgtt aaatgcgcat gtgcccagac
    16741 ggaaggtgtc accaacgaag tggtcaggat tgccagcgcg tagcgaaggg agcgtgcagt
    16801 tgaagtcata ttatctattc ctttttgtaa taactttttt acagagcata accttgtcta
    16861 atgtctgagt cgaggatcat caattccggc ttgccatcct ggctcactct tagtaacttt
    16921 tgcccgcgaa tgatgaggag attaagaatg ctgaagaatc tggctaaact ggatcaaaca
    16981 gaaatggata aagtgaatgt cgatttggcg gcggccgggg tggcatttaa agaacgctac
    17041 aatatgccgg tgatcgctga agcggttgaa cgtgaacagc ctgaacattt gcgcagctgg
    17101 tttcgcgagc ggcttattgc ccaccgtttg gcttcggtca atctgtcacg tttaccttac
    17161 gagcccaaac ttaaataaaa cttatacaga gttacacttt cttacataac gcctgctaaa
    17221 ttatgagtat tttctaaacc gcacccataa tttgcagtca ttttgaaaag gaagtcatta
    17281 tgtcgttctg gaaagttgca gcagcgcaat atgagcctcg taaaacctca ctcaccgaac
    17341 aggtagctca tcatctggag tttgtgaggg ccgccgccag gcaacaatgt cagctgttag
    17401 tttttccatc gctttcttta ctggggtgtg attattcgcg acgtgccctt cctgccccac
    17461 ccgatctctc actgttagac ccgctttgtt atgccgcaac gacctggcga atgaccatca
    17521 ttgccggcct tcccgttgaa tataacgatc gctttattcg tggaattgcg gtgttcgcac
    17581 cgtggcgaaa aacgcctggg atctaccatc agagtcatgg tgcatgtctg ggtcgtcgtt
    17641 ccagaaccat cacggtggtc gatgaacaac cgcaaggtat ggatatggac ccgacctgtt
    17701 cactgtttac tacagggcaa tgtctgggag aacccgacct gctggcgtcg gcccgtcgct
    17761 tacaattttt ttcacatcag tactcgattg ccgtgctgat ggcaaatgcc cgtggtaaca
    17821 gtgcactatg ggatgaatat ggtcgtctca tcgttcgcgc cgatcgtggt tcattattgt
    17881 tagtcggtca gcgttcatca cagggttggc aaggcgatat cattccatta cgctaggctt
    17941 tttcggcctg gagcatgcca tgttgcgcat tatcgataca gaaacctgcg gtttgcaggg
    18001 agggatcgtt gagattgcct ctgttgatgt cattgacgga aaaatcgtca accccatgag
    18061 ccacctggtg cgccccgatc gtcctattag tccacaagcg atggcgattc atcgcatcac
    18121 cgaagccatg gtcgccgata aaccgtggat tgaagatgtg atcccacact attacggtag
    18181 tgaatggtat gtcgcgcata acgccagctt tgaccgccgc gtactgcctg agatgcccgg
    18241 tgagtggatt tgcactatga aactggcccg tcgtttgtgg cctgggatca agtacagcaa
    18301 tatggcgtta tataaaacac gcaagctcaa tgtacagacg ccgccgggcc tgcatcatca
    18361 ccgcgcgttg tatgactgtt atatcaccgc cgcgttgctt atcgatatta tgaacacctc
    18421 cggctggacg gcagaacaga tggccgatat caccggacgt ccgtcgttga tgacgacctt
    18481 cacctttggc aaataccgtg gcaaagcggt ttccgacgtt gccgaacgcg atccgggcta
    18541 tctgcgctgg ttatttaata acctggacag catgagcccg gagctgcgtt taacactgaa
    18601 acattatctg gaaaatactt agtccgcagg cgtagcgggt aatgttccct gcgccagcgc
    18661 gaccagaaaa gcatattcca tcgctacgcc ttcgtacgat ttaaagcgac cagatttacc
    18721 gccatggcct gagtccatgt cggtacagag caataaaaga tggtcatcgg ttttcagctc
    18781 gcgcaattta gcgacccatt ttgccggttc ccaatattgc acctgagaat cgtgcaaacc
    18841 ggtcgttacc agtaaatgcg gataagcctg tgcggtgacg ttgtcatacg ggctgtagct
    18901 tttcatgtac tcgtaatatt gcggatcctg cgggttaccc cactcttcaa actcaccagt
    18961 ggtaagagga attgattcat caagcatcgt tgttacaaca tcaacaaacg gtacctgggc
    19021 gataacgccg tggaataatt ccgggcgttg attaattgca acgcccatca acatgccccc
    19081 cgcactcccg cccatcgcat aacaaagcga aggagagcca tagcccagtt ttaacaatgc
    19141 atcgcaggca tcaagataat cattaaacgt atttttcttc ttcagaaatt ttccgtcttc
    19201 gtaccattgt tgccccagct caccaccgcc gcgaacatgg acaatggcgt agacaaagcc
    19261 acgatctaac aaactcaagc ggctaaaact gaaatcggca tcaatacttg cgccgtaaga
    19321 accatagcca tacaccagca acgggttgtg tcctttgcga aaatgtttgc gatggtagac
    19381 caacgaaacc ggaacttcga cgccatcacg ggcgactatc cacaggtgtt cactgcggta
    19441 attcgccgca taaaaaccag gaacttccgt ttgttttaat acacgacgct caccggtatc
    19501 catatccagt tcaaacaaag tgtctggtgt agtcatggaa gaataaccat aacgcaatcg
    19561 cgcggtttca ggttctggat tgtaggcaat ccaggtcaca taggccggat catcaaaggc
    19621 aataccaatg acttcccggg tcttgcggtt aatttggcgc aaactggtta acccgcgctg
    19681 acgctcttca accaccagcc agtcggtaaa cagcgtaaac ccttccagca tgatgttttc
    19741 gcgtggcgga attaactctt cccactgttg ctcatcacgc atacgggtac ggtataagcc
    19801 aaagtttttg ccgtggcggt tggaacgcag ataaaaacga tgctggtagt gatcaaggct
    19861 gtattcgtga tctttgcggc gcggcagaaa aacaaacggc tcggcatcgg ccatttccgc
    19921 gtccagcagg cgaacttcac tggtggtggc gctggccaaa tgaatgacta catagtgctt
    19981 cgaggtcgtt ttatgcaggc tgacgtaata ggtatcgtct ttttcttcgt agatcagttt
    20041 atcatgcgat gctggcgtac cgatggcgtg acgccagacc tgataaggca gcagcgtcac
    20101 cggatgcttg cgaacatagt agaaagtcca ggagtcattt gcccagacaa agctgggttc
    20161 aacgttatcc agcagttccg ggtaccagtt accagtttcc agattacgaa aacgaatgcc
    20221 gtactggcgt cgggaaagaa aatcttctgc cagcgccata atggtgttat cgggcgtaat
    20281 cgccattccg cccatcgaat aaaactcact atgagctgcg cgcttattgg catcgagcaa
    20341 tgtttcccac tcatcccact cttcactgaa tgccgattga cgctggtaga tagcatattc
    20401 acagcctggt tcataaatat gccgatagcg gtagccattt ttgatgtagg gcgcagaaac
    20461 ttctcgttgc ggaatgcggt cgatgatttc ctttaagatg cgatcctgca aggcttgttg
    20521 tgaggccatc acccgatgac cgtaactatt ttcttgttgc aggtagtcca ggacttctgg
    20581 ctgagaacgc gtatcgtccc gcagccagta gtaattatcg atgcgcgtat cgccatgaag
    20641 cgtcatggcg tggggaatgc gggcggcttt tggtagcatg ttattgttct ttctggttga
    20701 aacatcttat aagggtggca aaactcaccg gggatgcaag cgaaacaggg gagtcattgc
    20761 ttagatgatg acaggtaatg gcgcggatat cgaatgttat gcagacagag aaaatcagcc
    20821 tgttcaaatg gctgtgcgat tctggatagc ccgaaatagt caacttcagg ctatccagag
    20881 agcggaatta ttccgccaaa gtgcgttttt gctgttcgag atcgcgttca atgccgtcac
    20941 gaacatcctg ggggattttc agcgcgtcac ccagtgcatt caggtaactg cgttccataa
    21001 aatggtcaat atcaatagcc gcgcaactca ggaaatagat ttccagcgcc tcttcttcat
    21061 tgcggacccc ggtagccagg cgttgtggat ccagcggttg ttcgattgcc tgctcaatga
    21121 gtacacgccc ctgctcttcc acgccggctt cacgcaattg ctggtcgatt gccgcacgtt
    21181 ctttggcatc aatatgacca tcacttttag cggcaaagac cagcgcaagg atcaaacgtt
    21241 ctgtacgctc atccagcggc atactttgcg cgccaaactg cggttcgtcc tgatgcgccg
    21301 cgcgaatttt atctttgtat ttattccaca gcaccgtacc cgctaccgct ccgccgccaa
    21361 ccagtaacgc gttggtgcca tatttcgtaa gaagtttacg tgctgattta tttgcgacca
    21421 gcagcccagc cagtccgcct aatgcgcctg gcactaacag tttgcccaat ccctgatccg
    21481 cagacgagga ggtagaagaa ctgctttgcc caagcaggga ttgcagttga tttaaccagt
    21541 tagccatatt tgctcctcaa taaccatttt tagtgcctgt agcatagcgg atgaggatgt
    21601 cagaaaatgt atatcgagac aaaagatgcg caaataagcg gggcggtagg ccgttattca
    21661 aagaaattcg cgccaggtaa ggtatttact gtatatcagg tgataagggt attttaggtg
    21721 aaagtagttt gtcgttttga gtggcattgt ttgatgctga ttgcccgatg cgacgctgtt
    21781 gcgtcttctc aggcctacga ttgtggtgcg ttctggggcg gacaaggcac tcgcgccgca
    21841 tccgccaacg gtcaatcgaa ttatcagaag ccattaacgc cgctgatatt ccgccattcc
    21901 cgctttgcag tccacgctga cctggtaatg aatatcggca cttttaccac gcacggttag
    21961 cggtactgac catttatcat ctttaccctg aatgtcctgc aaactgaccc aagccacggg
    22021 atcggcctga ccgacaattt tttgatcatc tgcccagcgc gccacgcgat tttgttgata
    22081 atcacgtttt acgctcgcgg caattccggc agcatccaga ccttcacact ttgggaaagt
    22141 gaccgacttg ctggtttcat tattggcagc gaaaactgat gcgcaggcag aaaccaacaa
    22201 cagccctaaa aacgcccctc tttttttcat gtttttctcc atagcacaat gattcaggag
    22261 aaagcatggt acaaattgtc aggagcgcaa gttgcttcag gccgcgtgtg aggcatcttc
    22321 ctcttcggat tcagcaccga cgacctgtgg ggacggtaat ttgccggttt tcagaatggt
    22381 gctcagcaca tctttttgtt ctgccagcca tagcgaaagg gcttcacgtt gctcgtcttc
    22441 catttgcact ggagaatttg tcagccaggt gtcaagcaga tccgccgtat caagcatttt
    22501 atcataagca tcggcttcct ttttgctggt aaacgacatt ttctcttcgc cctcacgaat
    22561 gactacgtat ttaacttcaa ccgccatttg cagcctctca taataactgt gattttatac
    22621 agtatatttc ttttcggttg agaaatcaac atcagcaata aagacacacg caaacgtttt
    22681 cgtttatact gcgcgcggaa ttaatcaggg gatattcgtt atgacgttat taggcactgc
    22741 gctgcgtccg gcagcaactc gcgtgatgtt attaggctcc ggtgaactgg gtaaagaagt
    22801 ggcaatcgag tgtcagcgtc tcggcgtaga ggtgattgcc gtcgatcgct atgccgacgc
    22861 accagccatg catgtcgcgc atcgctccca tgtcattaat atgcttgatg gtgatgcatt
    22921 acgccgtgtg gttgaactgg aaaaaccaca ttatatcgtg ccggagatcg aagctattgc
    22981 caccgatatg ctgatccaac ttgaagagga aggactgaat gttgtcccct gcgctcgcgc
    23041 aacgaaatta acgatgaatc gcgagggtat ccgtcgcctg gcggcagaag agctgcagct
    23101 gcccacttcc acttatcgtt ttgccgatag cgaaagcctt ttccgcgagg cggttgccgc
    23161 cattggctat ccctgcattg taaaaccggt gatgagctct tccggcaagg ggcagacgtt
    23221 tattcgttct gcagagcaac ttgctcaggc atgggaatac gctcagcaag gcggtcgcgc
    23281 cggagcgggc cgcgtaattg ttgaaggcgt cgttaagttt gacttcgaaa ttaccctgct
    23341 aaccgtcagc gcggtggatg gcgtccattt ctgtgcacca gtaggtcatc gccaggaaga
    23401 tggcgactac cgtgaatcct ggcaaccaca gcaaatgagc ccgcttgccc ttgaacgtgc
    23461 gcaggagatt gcccgtaaag tggtgctggc actgggcggt tatggtctgt ttggtgtcga
    23521 gctgtttgtc tgtggtgatg aggtgatttt cagtgaggtc tcccctcgtc cacatgatac
    23581 cgggatggtg acgttaattt ctcaagatct ctcagagttt gccctgcatg tacgtgcctt
    23641 cctcggactt cctgttggcg ggatccgtca gtatggtcct gcagcttctg ccgttattct
    23701 gccacaactg accagtcaga atgtcacgtt tgataatgtg cagaatgccg taggcgcaga
    23761 tttgcagatt cgtttatttg gtaagccgga aattgatggc agccgtcgtc tgggtgttgc
    23821 gctggcgacc gctgagagtg ttgttgacgc cattgaacgc gcgaagcacg ccgccggaca
    23881 ggtaaaagta cagggttaaa cccggcaaaa aaacgctaca aaaatgcccg atcgtagatc
    23941 gggcgttttg acttttacag cttagcgcct tctacagctt cacgcgccag cttagtaatg
    24001 cggtcgtaat cgcccgcttc cagcgcatct gccggaacca gccaggaacc accgatgcac
    24061 agcacgcttt tcagcgccag gtagtcacgg tagttagccg gagaaatacc acccgtcggg
    24121 cagaaacgga cctgggagaa cggacccgcg atcgcctgca gggctttcac gccgccgtta
    24181 gcttcagccg ggaagaattt gaactctttc aaaccgtagt ccatacccag catcagttcg
    24241 gaaacagtgc tgatccccgg aattagagga atagtccctt cggtagcagc tttcagcagc
    24301 ggctcggtca gacccgggct aattgcgaac tgtgcacccg cttcagtgac ttctgccagc
    24361 tgctgtggat tcagcaccgt accggcaccc acaatcgctt caggcacttc tttggcgata
    24421 gcacggatag cgtcaactgc acactcggta cgcagagtca cttccagaac gcgcacccca
    24481 ccagcaacca acgcttttgc catcggcact gcgtgttcca gtttttttac cacgataacc
    24541 ggtacaaccg ggccggtggt caggattgat tctgcacttg ttttccagtt tttcatcaga
    24601 gttttctctc gcctgattac aaatttgtcg tcttaaaaag tgatacaggt tgcgccctgt
    24661 tcggcaccgg acagtttttc acgcaaggcg ctgaataatt cacgtcctgt ccccacgcgt
    24721 gacgcgctca ggtcaggaat gcgcggttcg cgagcagcca gttccgcttc gtctaccagc
    24781 agcgtcagtt cgcctgtctg tccattcaca cgaatgatgt ccccgtcgcg cacttttgcc
    24841 agcagcccgc catcgtaggc ttctggtgtt acgtggatag ctgacggcac tttacctgaa
    24901 gcgccggaga gtcgtccatc ggtaactaac gcaattttga aacaccggtc caataataca
    24961 ccaagtggcg gcatgagttt atgtaattct ggcattccgt tcgcttttgg cccctgatga
    25021 cggacaacaa cgacacaatc gcggtccagc aaacccgctt caaaggccgg cataacgtca
    25081 tgctggcttt caaaaacaac cgctggcgct tcaatcacct ggttctcaac cggcacggca
    25141 gaggttttca taaccgcacg gcccaggtta ccgcttaaca cttttgtccc accatgatga
    25201 gagaaaggtt gttcgaagga agcgatcaca ttgctgtcga gtgatttttc cgccccttcc
    25261 cgccagtcca gttcaccatt attcagccat ggttcaaggg tataacgaga cagaccaaag
    25321 cctgccaccg tattgacatc ttcatgcagc aggcctgctt tgagcagttc acgcaccaga
    25381 accggtacgc cacctgccgc ctggaagtgg ttaatatcgg ccggaccgtt cgggtagaga
    25441 cgtgccatca gcggtacaac atcagaaagg tcagagaagt catcccagtt aatctgaata
    25501 ccggccgcgc gcgccatcgc caccaggtgc atggtgtggt tagtggaacc accggtcgcc
    25561 agcagtgcaa cgataccgtt caccaccact ttctcatcga tcatcttacc gatcggcatc
    25621 cattcattac cattaccggt catgcgtgta acctgacgcg cagcggcagc ggtcaaagca
    25681 tcgcgcagcg gagaatccgg atgaacaaaa gaagagcctg gcaactgcat ccccataaat
    25741 tccaccacca tctggttggt gttggcagta ccgtagaaag tacatgttcc cggcgcatgg
    25801 taagacgcgg cttctgactc cagtaaggcc atgcggtcca ctttaccttc gacataaagc
    25861 tggcgaatac gcactttttc tttatttggc aaaccgcttg ccatcggtcc agacggcaca
    25921 aacaccgcag gcaaatgacc aaacgacagg gctgccatcg tcagacccgg gacaatcttg
    25981 tcgcacacac cgaggaacag agcaccatca aacatgttat gggacagccc caccgccgca
    26041 gacatcgcta tcacttcgcg gcttagcagc gacaattcca ttccatcctg cccctgggtg
    26101 acaccatcac acatcgccgg aacaccgccc gcaacctgac caaccgcatt cgcttcatgc
    26161 agggctttac gaatgatttc tggatagtgt tcataaggct ggtgcgcgga gagcatgtcg
    26221 ttataggagg tgatgatggc gatattgtta cgcaacatgc ttttcaaaga ggctttgtct
    26281 tctggctggc aggcagcgaa accgtgtgcc aggttaccgc atgccaactg cgaacgatga
    26341 acggtcgacg ttttcgcttg ttctatccgg gcgagataag cagagcgagt ctcgcgcgaa
    26401 cgttcaatga ttcgatttgt tacgcgtaac aattgtggat tcataaaggc tcctgaaatt
    26461 gagttgtcag agcaggatga ttcacaacgc gtttcattca gaggatttat gagtgaaacg
    26521 cctgtaaccg gagctcatag ggcaaaaacg tttcagtcag tgtaataaaa aaagcctcgt
    26581 gggtgaatcc gcacgaggcc tgaaagtgta aaaattgttc tacaatctgt gcaagatcat
    26641 gttaccggta aaataaccat aaaggataag cgcagatatt actcaaactc attccaggaa
    26701 cgaccatcac gggtaatcat cgccaccgag gcaacgggtc cccaggttcc ggcctgatac
    26761 ggtttcggcg catcattgtc catcgcccac gcctcagtaa tggagtctac ccatttccag
    26821 gcttcttcca cttcgtcgcg acgaacaaac agtgcctgaa taccacgcat ggtttccagc
    26881 agcaaacgtt cataggcatc cgccagatgc gtctgattaa aggtttctga atagctcaga
    26941 tccagcttgg tgatttgcag gttatgtttg tggtcaaggc caggaacttt attcagtacc
    27001 tggatatcca cgccttcatc aggttgcaga cggatagtca gtttattctg cggcagatcc
    27061 tgccacgact ctttaaacag attcagttca ggtgttttga aatagaccac gacttcagaa
    27121 catttggtcg gcagacgttt accagtacgc aggtagaatg gcacaccggc ccagcgccag
    27181 ttatcaatgt cgacgcggat cgccacgaag gtttctgtat tgctgctctt gttcgcgccc
    27241 tcttcttcca gatatcccgg cacttttttg ccctgggcga agcccgcagt atattgcccg
    27301 cgtacggttt tttcgcgtac gttggagcgg tcgatgcggc gcagagactt cagtactttc
    27361 actttttcat cgcggatgct gtctgcgctc aggtcagacg gcggagacat cgcaatcatg
    27421 caaagaattt gcagcaggtg gttctggatc atgtcgcgca tctgaccggc tttatcaaaa
    27481 tagccccagc gcccttcgat ccccacttct tctgccacgg taatctcaac atgatcaatg
    27541 gtgcgattgt cccagttatt cacaaacagg gagttagcaa aacgcagcgc caacaggttc
    27601 agcaccgttt ctttaccaag atagtggtcg atacggtaaa cctggcactc ctcgaagtat
    27661 tcgccaacct gatcattgat ttcctgcgag gtcgccagcg acgtccccag cggtttctcc
    27721 atgactacgc gtgccggttt agcattcagt tttgcctcgc caagcccttt gcaaattgcg
    27781 ccaaaagtgc tgggcggcat ggcaaagtag ttaatggtga tacgattttt ttgatccagc
    27841 atcgcgccga gacggctgaa tgcagcagtg tcattgacat cgagattaca aaaatccaga
    27901 cgtgcactca gggtgtccca taaaccttca tcaatggttt ctttcatgaa agtttcgagc
    27961 gcctcgcgga caactttggt atatgccgct ttatcccagt cagcacgccc tacgccgata
    28021 atccgggtgt ccgggttgag ctgaccggct ttttccagtt gatacaggga aggcagcaat
    28081 ttacgacgcg caaggtcgcc tttcgcgccg aaaatgacca ggtcacaggc ctgggctgtt
    28141 tgcgttaccg ccatgtcatt ctccttaagt taactaaccc ggtacttaag ccagggtata
    28201 cttgtaattt tcttacggtg cactgtactg cttttacgag cttgcgaaaa ctgtaaacgc
    28261 ttatccaccc gtgcgattac gggaaaagcg cgcaaagtgc ggcaaaactg ataaaaaaat
    28321 catcgttttc cattgggtaa aaatctgaca ctgatcatgt tatgaaaaaa aataacaact
    28381 tttttatctg cttttgtcat taacacggca cacaggcgta atatctgaca aaactgcatt
    28441 tcgatttctt tcagtgcgga aatcgtcatt acccgtgagt ctctttacat catgaatatg
    28501 ctggaaaaaa tccagtctca gctggaacat ttgagcaaat cagagcgcaa agttgccgag
    28561 gtcattctgg cttcgcccga taacgcgatc cattcgagta ttgctgctat ggcactggaa
    28621 gccaatgtta gcgaaccgac ggtgaatcgt ttctgtcgca gcatggacac gcgcggtttt
    28681 cctgatttta aacttcatct ggcacagagt ctggcgaata gcactcccta tgttaatcgc
    28741 aatgtcaatg aagatgacag cgttgaatca tacacaggga aaatatttga gtccgcaatg
    28801 gcaacgcttg atcatgtccg tcattcactg gataaatctg ccatcaaccg cgccgtcgac
    28861 ttgctcactc aggcaaaaaa aatcgccttt ttcggattag gctcttcagc cgccgttgcc
    28921 cacgatgcga tgaataagtt ctttcgtttt aatgttccgg tggtgtactc cgatgatatc
    28981 gtgctgcaac gcatgagttg tatgaattgt agcgacggag acgtggtggt gctgatttct
    29041 cacactggaa gaacaaaaaa tctggtcgag ctggcgcagc tggctcgcga aaacgacgcc
    29101 atggtgattg ccctcacctc tgcgggtacc ccgctcgccc gggaagcaac gctggcaatt
    29161 accctcgacg taccggaaga tactgacatt tatatgccca tggtttctcg acttgcacag
    29221 ctgaccgtga tagatgtgct ggcgacagga tttactttgc gacgcggtgc aaaattcaga
    29281 gataacttga agcgggtcaa ggaagcgctg aaggaatcgc gttttgataa gcagttactt
    29341 aatttaagtg acgatcgcta aaaacgactg tcactgtcct aatcttatac gacatccgaa
    29401 tgagattaat ttatcgccat cgcggcgtta tttcattcgg atttcatgtt caagcaacac
    29461 ctggttgttt cagtcaacgg agtattacat gtccagaagg cttcgcagaa caaaaatcgt
    29521 taccacgtta ggcccagcaa cagatcgcga taataacctt gaaaaagtta tcgcggcagg
    29581 tgccaacgtt gtacgtatga acttttctca cggctcgcct gaagatcaca aaatgcgcgc
    29641 ggataaagtt cgtgagattg ccgcaaaact ggggcgtcat gtggctattc tgggtgacct
    29701 ccaggggccc aaaatccgtg tatccacctt taaagaaggc aaagttttcc tcaatattgg
    29761 ggataaattc ctgctcgacg ccaacctggg taaaggtgaa ggcgacaaag aaaaagtcgg
    29821 tatcgactac aaaggcctgc ctgctgacgt cgtgcctggt gacatcctgc tgctggacga
    29881 tggtcgcgtc cagttaaaag tactgaaagt tcagggcatg aaagtgttca ccgaagtcac
    29941 cgtcggtggt cccctctcca acaataaagg tatcaacaaa cttggcggcg gtttgtcggc
    30001 tgaagcgctg accgaaaaag acaaagcaga cattaagact gcggcgttga ttggcgtaga
    30061 ttacctggct gtctccttcc cacgctgtgg cgaagatctg aactatgccc gtcggctggc
    30121 acgcgatgca ggatgtgatg cgaaaattgt tgccaaggtt gaacgtgcgg aagccgtttg
    30181 cagccaggat gcaatggatg acatcatcct cgcctctgac gtggtaatgg ttgcacgtgg
    30241 cgacctcggt gtggaaattg gcgacccgga actggtcggc attcagaaag cgttgatccg
    30301 tcgtgcgcgt cagctaaacc gagcggtaat cacggcgacc cagatgatgg agtcaatgat
    30361 tactaacccg atgccgacgc gtgcagaagt catggacgta gcaaacgccg ttctggatgg
    30421 tactgacgct gtgatgctgt ctgcagaaac tgccgcaggg cagtatccgt cagaaactgt
    30481 tgcagccatg gcgcgcgttt gcctgggtgc ggaaaaaatc ccgagcatca acgtttctaa
    30541 acaccgtctg gacgttcagt tcgacaatgt ggaagaagct attgccatgt cagcaatgta
    30601 cgcagctaac cacctgaaag gcgttacggc gattattacc atgaccgaat cgggtcgtac
    30661 cgcgctgatg acctcccgta tcagctctgg tctaccaatt ttcgccatgt cgcgccatga
    30721 acgtacgctg aacctgactg ctctctatcg tggcgttacg ccggtgcact ttgatagcgc
    30781 taatgacggc gtagcagctg ccagcgaagc ggttaatctg ctgcgcgata aaggttactt
    30841 gatgtctggt gacctggtga ttgtcaccca gggcgacgtg atgagtaccg tgggttctac
    30901 taataccacg cgtattttaa cggtagagta agtacgttgc cggatgcggc gaaaacgcca
    30961 catccggcct acagttcaat gatagttcaa cagatttcga atattctgaa gcaaacttga
    31021 acttatcatc aggcgaaggc ctctcctcgc gagaggcttt tttatttgat gggataaaga
    31081 tctttgcgct tatacggctg gatttcgccc ggtttgcgag ttttcagcaa ttttagtatc
    31141 caggtgtatt gttctggtcg cggaccaaca aaaatctcga cttcttcatt catccgccgc
    31201 gcaatcgtat gatcatccgc ctctaacaga tcatccatcg gtgggcgcac ctgaatcgtc
    31261 agacggtgcg tcttgccatc ataaatcgga aacagcggaa caacgcgcgc acggcacact
    31321 ttcatcaaac gaccaatcgc gggcaacgtc gctttatagg tggcaaagaa atcaacaaat
    31381 tcgctgtgct cagggccatg atcctgatcg ggtaaataat atccccagta gccctgacgt
    31441 accgactgga tgaatggttt aataccatca tttctcgcat gcagacgacc gccaaaacga
    31501 cgacgcaccg tgttccagac ataatcaaaa accgggttgc cctgattatg gaacatcgct
    31561 gccattttct gcccttgcga ggccatcagc atggcaggaa tatcgacggc ccaaccgtgc
    31621 ggcaccagaa agataacttt ctcgttatta cgccgcatct cttcgatgat ctccagccct
    31681 tgccagtcaa cgcgcggctg aattttctcc ggcccgcgta ttgccaactc agccatcatt
    31741 gccatcgctt gcggcgcggt ggcaaacatc tcatcaacaa tcgcttcgcg ttcagcttca
    31801 ctacgttctg gaaagcagag cgacagattg attaacgcac gacggcgtga gctttttccc
    31861 agtcgtccgg caaaacgtcc cagccgtgcc agaatgggat cacggaactt tggcggcgtt
    31921 aaagcgatac ccgccatcgc tgctacgccc agccatgctc cccagtagcg cgggtggcga
    31981 aaggatttat caaactcagg aatgtattcg ctattatttt ttttcgtttc catgcttttc
    32041 cagtttcgga taaggcaaaa atcaatctgg tgatagtgta gcggcgcaac ttgccccgca
    32101 ccaaataaaa aaagccggta ctgacttcgt accggctgcg aatggatgtt aattaatcaa
    32161 accgtagctg cggcacaatc tctttggcct gcgccaggaa ttcgcgacga tcggagccgg
    32221 tcagcccttc ggtacgcggc agttttgccg tcagcgggtt tacggcctgc tggtttatcc
    32281 atacttcata gtgcaaatgc ggcccggttg aacgtccggt attaccggaa agcgcgatac
    32341 ggtcgccacg tttcaccttc tgtcccggtt tcaccagaat cttgcgcaag tgcatataac
    32401 gcgtggtgta gctgcgacca tgacgaatag ccacataata acctgctgcg ccactgcgtt
    32461 tggcaaccac cacttcaccg tcacccactg aaagcactgg cgtaccctgc ggcatggcga
    32521 aatcaacacc tctgtgtggt gcaacgcgac cggtcaccgg attagtacga cgcgggttaa
    32581 agttagacga gatacggaac tgtttcgccg tcgggaatcg caagaatcct ttcgccagac
    32641 cagtaccgtt acgatcgtag aatttgccat cttcagcgcg gatagcgtaa taatctttac
    32701 cttctgaacg caaacgtacg cccagcagct ggctttgctc acgtttacca tcaagcattt
    32761 ctcgagacat taataccgca aattcatcgc cttttttcag tttgcggaaa tccatttgcc
    32821 actgcatggc tttaatcact gcgctcactt cggcgctggt taaaccggcg tttctggcgc
    32881 tggcaacaaa gctccccccg acggtacctt tcagcagatt gttaacccac tctccttgct
    32941 gcatttcgct ggtcatttta aaaccgttag cggcagtacg gtcataggtt cgggtttcac
    33001 gacgagacac ttcccaggtg aggcgctgca gttcgccgtc cgcggttaat gtccaggaga
    33061 gttgttgacc gattttcagg ttacgcaatt ctttgtcggc agcagccagt tgggtgatat
    33121 cacccatatc aataccatac tgattgagaa tgctgcttag cgtatcgcca gtggaaacaa
    33181 catattcatg cacgcccgct tcaccggcga ttttgtcatc cagttcgtcc tgaggaatgg
    33241 cttcatcttc ttgtgcagct tgatcaatcg gctcactggc ttcaggtaag agcgaacgaa
    33301 tttcgttctg ttccagctca atggttttga caattggcgt ggcatcgcgg tgataaacat
    33361 agggccgcca gacagcgacg gccagagtaa gaacggtgag cgaccccaac ataacgcggt
    33421 gtggtcgcgg taaattatta aacgccaggg cgacagagcg ggctatctgt tgcacgtaat
    33481 cacttcctca ttaatctcct ttcaggcagc tcgcatactg gttggctaat tgactcagga
    33541 attctgaata gcttgtttta cccagtttga tattcgtccc caggggatcc aacgttccca
    33601 tacgaacgga tgtccctcgt gcgacgctct caacgaccgc tggcctgaac tgtggctcag
    33661 caaaaacgca ggttgctttt tgctcaacca actgtgttct tatttcatgt aaacgctgcg
    33721 cgccaggttg aatctcaggg ttaacggtaa aatgaccaag cggtgtcagt ccaaactgtt
    33781 tttcgaaata gccgtaagca tcgtgaaaaa cgaaataacc tttccccttg agcggcgcga
    33841 gctcgttacc aacctgcgtt tcggttgagg ctaattgtgc ctcaaaatcc ttcaggttgg
    33901 cgtcaagttt ggctcgactt tgcggcataa gttccactaa ttttccatgg attgcaaccg
    33961 ctgtagcccg cgctatctct ggggaaagcc aaagatgcat gttgaaatcg ccgtgatggt
    34021 gatcttcgtc acttttttcc gcgtggtcgt gatcatcatc atcgccgtga atacttttca
    34081 tcagtagcgg tttcacatct tcaagctgcg caatcgttac ctgcttcgct tctggtaatt
    34141 tgcttaccgg tttttgcata aacgcttcca tctccgggcc aacccaaacg actaagtccg
    34201 cgttctgtaa gcgttttaca tccgatggac gcagtgaata atcatgttct gaagcgccgt
    34261 caggcagtaa aacctccgtt tctgttacac catcagcaat ggcagaagcg atgaacccaa
    34321 cgggtttaag cgaagcgaca acggcggcat ctgcggcctg tgttgcacct ccccagagag
    34381 cggcggataa tgctgcgaaa agaagcgttt ttttatgtaa cataatgcga ccaatcatcg
    34441 taatgaatat gagaagtgtg atattataac atttcatgac tactgcaaga ctaaaattaa
    34501 catgacaagt ctggtttccc tggaaaatgt ctcggtttct tttggccaac gccgcgtcct
    34561 ctctgatgtg tcgctggaac ttaaacctgg aaaaattttg actttacttg ggccaaatgg
    34621 cgcaggtaag tcgacactgg tacgggtagt gctcgggctg gtaacacccg atgaaggggt
    34681 tatcaagcgc aacggaaaac tgcgcatcgg ctatgtaccg cagaagctgt atctcgacac
    34741 cacgttgcca ctgaccgtaa accgtttttt acgcttacgc cctggtacac ataaagaaga
    34801 tattttgcct gcactgaaac gtgtccaggc cgggcacctg attaacgcac cgatgcaaaa
    34861 gctctctggt ggcgaaacgc agcgtgtact attagcgcga gcattgttaa atcgcccgca
    34921 attattagtg ctggatgaac ccactcaagg cgtggatgta aatggccagg tggcgttata
    34981 tgaccttatt gaccaactgc gtcgtgaact ggattgtggc gttttaatgg tttctcacga
    35041 tctgcatctg gtgatggcaa aaaccgatga agtgctgtgc ctgaatcacc acatttgttg
    35101 ttccggcaca ccggaagttg tttccctgca tccggagttt atttcaatgt ttggtcctcg
    35161 tggtgctgaa caactgggta tctatcgcca tcatcataac catcgtcacg atttacaggg
    35221 acgaattgtt ttgcgtcggg gaaatgatcg ctcatgattg aattattatt tcccggttgg
    35281 ttagccggga tcatgctcgc ctgtgccgcg ggtccgctgg gttcgtttgt agtctggcgt
    35341 cgtatgtctt atttcggtga tacgctggct catgcctcat tacttggcgt cgcgtttggt
    35401 ttgttgctgg acgtgaatcc attctatgcg gtaattgccg ttacgctgct cctggcgggc
    35461 ggtctggtat ggctggagaa gcgtccacag ctggcgattg acacgttatt agggattatg
    35521 gcgcacagtg ccctgtcgct gggcctggtg gtcgttagtc tgatgtctaa cattcgtgtt
    35581 gatttgatgg cttacctgtt cggtgatttg ctggcagtga cgccagaaga tctcatcgct
    35641 attgcgattg gcgtggtcat cgtggtggct attttgttct ggcaatggcg caatttgctg
    35701 tcgatgacga ttagcccgga tctggcgttt gttgatggtg tgaaattaca gcgcgtgaaa
    35761 ttgttgttga tgctggtgac ggcattgacg attggtgtag cgatgaaatt tgttggcgcg
    35821 ttgattatta cttcactgct gattattcct gcagctactg cgcgtcgctt tgcccgtacg
    35881 ccggaacaga tggctggtgt cgctgttttg gtggggatgg tggcagtaac tggcggttta
    35941 accttttccg cgttttacga tacgccggcg ggtccgtcgg tggtgctatg tgcggcactg
    36001 ttatttattc tcagtatgat gaaaaagcag gccagctaat ctatcgctga acacatttgt
    36061 cggatgcggc gcgagcgcct tatccgacct acggttcgct atctctggta ggccggataa
    36121 gacgcgaata gcgtcgcatc aggcacactg ccaatgtcgg atgcggtgcg agcgaccaat
    36181 ccgacttacg gcatttctgg cggcgttatg ccaaagtgat tccacgcccg cgtcgtcgcc
    36241 atacgcccac gcggtgtacg ctgcaaaaag ccttgctgaa tcagataagg ctccagcaca
    36301 tcctcaatgg tttcacgttc ttcgccaata gccgccgcca ggttatccag acctacaggc
    36361 ccaccaaaga atttatcgat taccgccagc aacaatttgc ggtccatata atcaaaacct
    36421 tcagcatcga cattcagcat atccagcgcc tgagcagcga tatctgccga gatggtgcca
    36481 tcgtgcttca cttcggcgaa atcacgcact cgacgcagca gacggttggc aatgcgcggc
    36541 gtaccgcgag cgcgacgagc aacttccagc gcgccgtcat cactcatctc aagccccata
    36601 aagcgtgcgc tgcgactgac gatatattgc agatccggca cctgataaaa ctccagacgt
    36661 tgcacaatac caaaacggtc gcgcaacggt gatgtcagcg aacctgcgcg tgtggttgca
    36721 ccaatcaggg taaacggcgg caaatcaatt ttaatggagc gtgccgccgg accttcacca
    36781 atcatgatat ccagttggta gtcttccatt gccggataca acacctcttc aacaactggc
    36841 gatagacggt ggatctcatc aataaacagc acgtcatgcg gttcaaggtt agtgagcatc
    36901 gctgccagat cgcccgcctt ttccagcacc ggaccagaag tcgtgcgtaa attaacgccc
    36961 atttcattgg cgacaatgtt ggcaagcgta gttttaccca accccggagg accaaaaatc
    37021 aacaaatgat cgagggcatc gccgcgcagt ttcgctgctt tgatgaaaat ctccatctgt
    37081 gaacgaacct gcggctgacc aacatactct tccagtaatt tagggcgaat ggcgcgatct
    37141 gctacatctt ccggcaaagt ggtaccggca gaaatcagac ggtctgcttc aatcatcctt
    37201 tacctcataa cgcggcgcgt agggcttcgc gaattaatgt ttcactgctg gcgtcagggc
    37261 gagcgatttt gctcaccatg cggcttgctt cttgtggttt atagcccagc gccaccagcg
    37321 cggcaaccgc ttcttgttca gcatcgtcgg tcgccgggct ggcaggagac gtgagcacca
    37381 gatcggcggc tggcgtaaag agatcgccgt gcaaaccttt aaatcggtct ttcatttcga
    37441 caattaaacg ctcggcggtt tttttaccaa tgcccggcag tttcaccagt gctcccactt
    37501 cttcacgttc aacggcatta acgaactgct gcgctgacat tccggagagg atcgccagcg
    37561 ccaacttcgg gccgacgccg ttggttttga ttaactcttt aaacaacgtg cgctcttgct
    37621 tattgttaaa gccgtacagc agctgcgcgt cttcacgcac cacaaagtgg gtgaaaacga
    37681 tcgcttcctg acccgcttca gggagttcat aaaaacaggt catcggcata tgcacttcat
    37741 agcctacgcc gcccacttca attaacacca gcgggggttg tttttcaatg atgatgcctc
    37801 tgagtctgcc tatcacatga cgctcctgcg taatgaatca aagataatgc tgtatgataa
    37861 aaaaatgctg gatagatatc cagcgaagga tgaagaaaac ttgcgaggcg tcccaacgat
    37921 ctgaaaaatg gcgcagtata atttattcta caggttatat tggaagcaaa tatttgaata
    37981 ttacatattc agtgaagaaa tgcgtaataa aaatatacat tgcgactcct gaaataataa
    38041 atttttta
//
LOCUS       DAZFQD010000034        36481 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.034, whole genome shotgun sequence.
ACCESSION   DAZFQD010000034 DAZFQD010000000
VERSION     DAZFQD010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 36481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36481
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.034"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            45..779
                     /locus_tag="ACHKTW_001793"
     CDS             45..779
                     /locus_tag="ACHKTW_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFO6462832.1"
                     /translation="MKSIATLVVCAISGIACVNLSAHAAEGNHTISLGYAHFQFPGLK
                     DFVKDATAHNRETFSHFVNRNYFSSLGEYTDGRVSGYEGKDKNPQGINIRYRYEITDD
                     FGVITSFTWTRSLTNSQTFIDVQSADHTRKIKNPAASARTDIRANYWSLLAGPSWRVN
                     QYMSLYAMAGMGVAKVSADLKIKDNINSSGGFSESNSTKKNSLAWAAGAQFNLNESVT
                     LDVAYEGSGSGDWRTSGITAGIGLKF"
     gene            1010..1150
                     /locus_tag="ACHKTW_001794"
     CDS             1010..1150
                     /locus_tag="ACHKTW_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO6462833.1"
                     /translation="MSQKSLITVTICMTVIFTIWMLHGSLCEFRLNLWGAEFAAFLQC
                     KQ"
     gene            1207..1608
                     /locus_tag="ACHKTW_001795"
     CDS             1207..1608
                     /locus_tag="ACHKTW_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6462834.1"
                     /translation="MAPVSGERMNDKILRYMQRVVRNSRNPEFMNEVKDACLKKQAFC
                     FEAPDGFLVLRSVLSADGIPYVLVLLGVCTGSNSVERYLPEVKTLTRLAGGRWAEFHT
                     ARRGFIRLGKRLGFERMPDDEDGFMVFRIAV"
     gene            1703..2359
                     /locus_tag="ACHKTW_001796"
     CDS             1703..2359
                     /locus_tag="ACHKTW_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6462835.1"
                     /translation="MGGSKGGGDTKVKPTAAQIAQEEVAWKGWQDYKNILRPAEDNFM
                     EKVDDLNSEQQYDNIAGTTNLGYQKQFGEARKELAGNLAQSGVDPSSGRFNAVMNANQ
                     SDQVTGQIDTTTRGQVSQADKYVAGLQDVAALGSGQKADALQSFNSLADSSLAKAKSD
                     AQAAFTKQQGRASLVGAGLGAVGAYAMHKAGGSGGSGGAKTPGTGANAIQHQAQNWRL
                     "
     gene            2362..2808
                     /locus_tag="ACHKTW_001797"
     CDS             2362..2808
                     /locus_tag="ACHKTW_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6462836.1"
                     /translation="MEYGKYETLARYGYTGAARPQGDWQTSAALTRQQYDDWRTRYLP
                     RVARLADLGENNSLMNAQLARVGGLATSSLRTAQMAQDNQMARYGVSRPDNPDSNTLG
                     LRNALAIAGAKNGIREAEQDRQMNILTGASAPARQKLSVGGQLVAA"
     gene            2818..3069
                     /locus_tag="ACHKTW_001798"
     CDS             2818..3069
                     /locus_tag="ACHKTW_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HFO6462837.1"
                     /translation="MGYGLLDIANQSRREALQGISDADRRREEIEAANKQMAAQQKAQ
                     NKQNIGTGIGTGAAIGASVGGPVGAVAGAVIGGIAGSLF"
     gene            3080..4345
                     /locus_tag="ACHKTW_001799"
     CDS             3080..4345
                     /locus_tag="ACHKTW_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6462838.1"
                     /translation="MSGFAQGLLAGFSTVDQAMTRRKELGLREAQLARQQKNNERDFE
                     FAQSQFEHNKNVDQRNFDYRAKVDDRNYALKEREFNANQNYRNASLGMEQQRLRMQKY
                     NQRRLEYNDMLARDQPVMAALGKAVDAGDRDAAMRLYGQLSEGNPLRLMANDGYVAKA
                     GQAVNNLQKIFDDKPDRAIASLNTPENLDVLSGVFGPELQQRIGMPDSTGKKTIKEAR
                     IGSIVPAQQEGYILIGLDLTYSDGSTAHKPVTEYGSAHPDDQTVLAIPVDKAIAQVRD
                     RSKFAEISKNYGYFMPKQQGLSLKELQKGASNVAADAIKNGGNAQAAVDEYYAATGSQ
                     PHQQKIQQQKLQQQVINWAGDDPDKLSFARNVAARQPEMLEPQNQKLLENGYANFLRI
                     QKARGEQARDESASSASQFIRGLKQNYAQ"
     gene            4415..12796
                     /locus_tag="ACHKTW_001800"
     CDS             4415..12796
                     /locus_tag="ACHKTW_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPD38 domain-containing protein"
                     /protein_id="HFO6462839.1"
                     /translation="MAYSEEQRPEAQLGNQNRNSLNIQQPGETDSYEAFFSDPNRWKD
                     NSTSFSLGDVLPTMGKGFAQSVRGTGEMARGLGDAMIQSPVKTGARILNEFSRMGLPG
                     VATVQDIFAGGSRGADEVIDTLPDGKNAVTDTVGKGLKATGKAVSDGAEATDEWLTGK
                     MSPGAVRALNTPMTEGYDDSAVWVAKGVNLIGALVPDMVAGGVAKKVGDVTLRKMLTA
                     GLEKKYIAAGMQPERATALAAEAVDKKMPDLFQAGLITHSTASAQGQSAMAAADAVLN
                     ADYSELAQSPKFQQTFLSIDADPQHAQLTDRQKMDLAKERVADEVRAQLATDPELLAV
                     NAMAAKLGDAQLFNLVTRGTAKTVKSGIVRNATEQGAINAAQGGYSRYQENTALRETA
                     GMDVSPWEGVADATIEGAAFGAAMGAPSGAVAGYRGRRQAAEEAAMRDAETVQQDDAA
                     PQPESVDPVAQHRESMQGMNREQLLEQYADADMATEGDASAAHRREAASQLLNELDEQ
                     AKRQAVMNELKAKPRSELLEEYRRLSQKEERTETEEQQFQAIREVIRPQQEVTPEAQS
                     QPENAEDGNGSIYPTVRFRDPNEVRIEINGNGASRPAERIEKVRPDNRYFTDEKSAMG
                     SDVFRNAAATGLKPSVVKKGETQYAVEMDNPAFSEDVATETINTLADGERIADADPME
                     QPAFMRDPRFRGFTGDDTEVQARLARGNVPTAEELVRSQMAEGDAGPTAQELTERPRL
                     PAPGDIHPGQGYPLPGEVARTPDENQAGRGGRFTTTGEVKGQSFQKGQALAPENAAGR
                     QGETLEGEMVRRGLPSPDAQNATAPVREGLPAPDITHNVRMPQPDQLPRTVRNSLPEL
                     AQQAEVRRQAGGNRDIPQPETIAPESETTVATDREATVRGGEVRGKKIEDFGEEIKGA
                     AKHRYAQLAETLGKTLEDRDYVTQPLSKLFPKPDYAKLASEGADADTLAMIALYRSDI
                     PVKTKHNTAGWGESVKKVRHSVSEMLNGTVSAKRLAEWMEGRMPSRYADTWQLLRTLP
                     PSQMDKASAYRVVSGVYQAAGGKRYDPPQKLYSLRNKDNKGSNLFFSESRDELLAKAK
                     VWFAEQEEKSQAKGDEKTAPSPDDKIRFDVYRNTRSGDIFIAYGKNKMRVRGGFKSAS
                     DARKYIDLHRDELVRHVKEMREISREEQRNATNRDRTGPERRKGDVSPEQFSDAFGFR
                     GVQFGNYVESPRRQADLNRAYDSLHDLADVLNVPTKALSLNGRLGLAFGARGKGKAAA
                     HYESGEVAINLTKGNGPGALAHEWFHSLDNYFGRYDVSSDGKITSGGDFMTEAQRVRR
                     IFKDGRYVDAEYPVRQEVYDAFKGVIQAIKNSDMPRRSALLDEVRSKPYWSTDVEMAA
                     RAFERYVQDKARMAGVENDYLVNIRKAPEHNTDNTYAYPTNAELDGGIREAFDHLFRT
                     LKTRETDKGVAFYSRKGMTRTPEGNLISDVNRSAEAKGSPVPQVEAVARGVMSGIKDS
                     DLTVRVVKSQKEAEALAGESFDGYGRVHAFYRPDKREIVLVADNIPDGQTVREKLRHE
                     IIHHAMEHVVTPAEYQTIIKTVLKTRDSDNATIREAWRKVDASYGKESPEVQAGEFLA
                     HMAEKQPNKFVAAWERVVALVKGVLRRTGLLKPTELNDIRLVRETIRTLGQRVREGYT
                     PREDGVGASSQYSRSGKPDPFKVPEGEGERYRDDLARMMKSLRTTDLTVNIGRTPPVL
                     RHLGAPDLPLVISRDTVRKATNGVKHVVPMDVIERLPELMHDPDAIYRSATERNAVVM
                     LLDAVDKNGDPVVSAVHMKATQKLLEVNRIASVYGTENGKKLKSMETAGLTLYRREKL
                     NPDGSLYRGLQLPKDEHSRQGSADKILYPEDIRKGPYYSRTSSLTPEETIASRFVRQM
                     QDKFQVLKAVQENIRKTGGKIDDSNNAYMAEELFHGKAENDLNVMKERYVQPLAKLLA
                     DYKIAQADLDEYLYARHAPERNAHIAKINPKMPDGGSGMTNAEAAEIMQRVRNSGKQA
                     QYDRLAGIVDDMLARRRELIREAGLEESGVVDAWQKAYRYYVPLKGQDVDGVVSLPRT
                     GKGFTIGGRESKQAMGRASRAQSPSTQAIQDLSESLIRNRKNEVGNAFLKLVQDNPDK
                     DYWQVFTDDRPDTMRTIAERKDKETGETIREVVERPVPMAMMADRYFTTKKNGKTYYI
                     KLHDPRLMRAMKNMGPETSNAVIRTLGKVNRFLATVNTSYNPEFLVSNFIRDVQTAVM
                     NLKAEQGRSDGKLKGLDNLSALAVVKDSRSAMSAVYASLRGKTLTGNGAQWQKVWKEF
                     VEDGGKTGWFNMGDLEGQQKEMDRLVSLAKGGWKGQSIGAWNSFLNLVEDANGAVENA
                     LRLSAYKHARDAGLSRQQAASLAKNMTVNFNRRGEQGALMNSLYMFANASIQGTANLV
                     RTLGHLNGEGPLLERLRWKNLNVPQKIALAAVGAGYLLGSLNRSVAGEDDDGVNWYDK
                     VPSHVKERNLVIMKSVFGGKAGEYWSIPLPYGYNVFFLLGHTAEGVAAGDLTASRAAG
                     NVVGGVLGAFSPVGSETSETLSGALLKNAAPTILRPFANLAMNENFMGAQIYQENMPF
                     GTPKPDSQLGRRSTPEAYKAFASWLNAFSGGSQYRPGAVDITPESLKFWIDYISGGTG
                     RFISKTTDAAVKSLNGIDIPEQQVPFLGKISGEVMPYADQQKMYDRMTEIAQYHAELK
                     SLTGAERTAFIDENNGKLSMNGLMQDTRKRLKDLRKQRDAIYADSSLSLAQQAAIVKS
                     VERDMKVAVDRFNREYNKKVGVD"
     gene            12920..13111
                     /locus_tag="ACHKTW_001801"
     CDS             12920..13111
                     /locus_tag="ACHKTW_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000481379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6462840.1"
                     /translation="MFNEEKVAQMAAYLLKKHGGSMRFIKLMKLMYLSDRKAMESLTG
                     KGRGLNAPPQFPGIKSPCR"
     gene            complement(13198..13413)
                     /locus_tag="ACHKTW_001802"
     CDS             complement(13198..13413)
                     /locus_tag="ACHKTW_001802"
                     /inference="COORDINATES: protein motif:HMM:NF016343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFO6462841.1"
                     /translation="MPEMGERQYYVLAADFKNNYPPSIPNTQVGVYGDWFNDGNPTWD
                     DGDGEDLHLKEVTHWMPLPEPPKEVSQ"
     gene            complement(13501..14124)
                     /locus_tag="ACHKTW_001803"
                     /pseudo
     CDS             complement(13501..14124)
                     /locus_tag="ACHKTW_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000208055.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
     gene            complement(14121..14453)
                     /locus_tag="ACHKTW_001804"
     CDS             complement(14121..14453)
                     /locus_tag="ACHKTW_001804"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6462842.1"
                     /translation="MADRKKRFRKNPSLGMGDWRFFISEPGIISIEDLPPGWGLLHVV
                     NGRVRKVHGWPKGNCCWGNPEDKPFIGNKQVECDYMLSALRRMELRGHLNEIYDGVIV
                     NKKEGNAA"
     gene            complement(14458..14636)
                     /locus_tag="ACHKTW_001805"
                     /pseudo
     CDS             complement(14458..14636)
                     /locus_tag="ACHKTW_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305741.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(14638..15588)
                     /locus_tag="ACHKTW_001806"
     CDS             complement(14638..15588)
                     /locus_tag="ACHKTW_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFO6462843.1"
                     /translation="MSEINYQVLREKAEKATRGEWSLEYGENRFDGDDALIHREAAGY
                     IPICRIEGAHPESGFDEDFQMEQQANAEFIAAANPATVLALLDERERNQQYIKRRDQE
                     NEDIALTVGKLRVELETAKSKLNEQREYYEGVIADGSKRIAELEKQCAEWERKALSNF
                     EECAAMAERIEEMQTKSAPDSFGIIGENIRTQDNRITSDPMFCVYQKREIVVDADYDH
                     DRIVWVDEDGNEANKRHSRRLELLHENFREPPEKWRRVAVKDIDEFVTCCFTEQGCKD
                     YLAVNGHNLRLPFIYVKSGFRNAEYIGIRNWLAGIRIKGE"
     gene            complement(15575..15859)
                     /locus_tag="ACHKTW_001807"
     CDS             complement(15575..15859)
                     /locus_tag="ACHKTW_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4752 family protein"
                     /protein_id="HFO6462844.1"
                     /translation="MSIDTTITIDTALNTGLALLGYFYIMFCAGRWLSLLLLKKWNKC
                     RKQDQRQKAMNAFSEAFGIDSMEPGDPARVISRGDVVILVYRSEEKNERD"
     gene            complement(15856..16077)
                     /locus_tag="ACHKTW_001808"
     CDS             complement(15856..16077)
                     /locus_tag="ACHKTW_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HFO6462845.1"
                     /translation="MADIIDNAAEIEELQRNLSLQKYKSESNAPSATHCCECGDPIDE
                     RRRQAVRGCRTCASCQQDIELINKQRGVK"
     gene            complement(16125..16748)
                     /locus_tag="ACHKTW_001809"
     CDS             complement(16125..16748)
                     /locus_tag="ACHKTW_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000184489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor Ant"
                     /protein_id="HFO6462846.1"
                     /translation="MTTQLIPVFEGTISNEPTLLVNARDLHGFLEVGKDFSNWIRAKL
                     NEYGFVENLDYILFSPNLAKTPGRRRKDYHLTLDTAKELAMVERNEKGRQIRRYFIEC
                     EKKLRNIQPVQTEQQFTAEDIILLCYVQLWMEKAQQLSKQLYPVMRELNSDYYGKLFD
                     LAYETRHITNRTRDTLLREAAKLDPANYMVHRARSMLAQLRARQFEF"
     gene            complement(17016..17285)
                     /locus_tag="ACHKTW_001810"
                     /pseudo
     CDS             complement(17016..17285)
                     /locus_tag="ACHKTW_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203821.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antA/AntB antirepressor family protein"
     gene            complement(17700..17804)
                     /locus_tag="ACHKTW_001811"
                     /pseudo
     CDS             complement(17700..17804)
                     /locus_tag="ACHKTW_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
     gene            complement(17815..20238)
                     /gene="ynfF"
                     /locus_tag="ACHKTW_001812"
     CDS             complement(17815..20238)
                     /gene="ynfF"
                     /locus_tag="ACHKTW_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate/tellurate reductase subunit YnfF"
                     /protein_id="HFO6462847.1"
                     /translation="MKIHTTEALMKAEISRRSLMKTSALGSLALASSAFTLPFSQMVR
                     AAEAPVEEKAVWSSCTVNCGSRCLLRLHVKDDTVYWVESDTTGDDVYGNHQVRACLRG
                     RSIRRRMNHPDRLKYPMKRVGKRGEGKFERISWDEALDTISDNLRRILKDYGNEAVHV
                     LYGTGVDGGNITNSNVPYRLMNSCGGFLSRYGSYSTAQISAAMSYMFGANDGNSPDDI
                     ANTKLVVMFGNNPAETRMSGGGVTYYVEQARERSNARMIVIDPRYNDTAAGREDEWLP
                     IRPGTDGALACAIAWVLITENMVDQPFLDKYCVGYDEKTLPANAPRNAHYKAYILGEG
                     PDGIAKTPEWAAKITSIPAEKIIQLAREIGSAKPAYICQGWGPQRHSNGEQTSRAIAM
                     LSVLTGNVGINGGNSGVREGSWDLGVEWFPMLENPVKTQISVFTWTDAIDHGTEMTAT
                     RDGVRGKEKLDVPIKFLWCYASNTLINQHGDINHTHEVLQDDSKCEMIVGIDLFMTAS
                     AKYCDILLPDLMPTEQEDLISHESAGNMGYVILAQPATSAKFERKPIYWMLSEVAKRL
                     GPDVYQTFTEGRSQHEWIKYLHAKTKERNPEMPDYEEMKTTGIFKKKCPEEHYVAFRA
                     FREDPQANPLKTPSGKIEIYSERLAKIADTWELKKDEIIHPLPAYTPGFDGWDDPLRK
                     TYPLQLTGFHYKARTHSSYGNIDVLQQACPQEVWINPIDAQARGIRHGDTVRVFNNNG
                     EMLIAAKVTPRILPGVTAIGQGAWLKADMFGDRVDHGGSINILTSHRPSPLAKGNPSH
                     SNLVQIEKV"
     gene            complement(20299..21333)
                     /locus_tag="ACHKTW_001813"
                     /pseudo
     CDS             complement(20299..21333)
                     /locus_tag="ACHKTW_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416104.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
     gene            21391..22974
                     /locus_tag="ACHKTW_001814"
     CDS             21391..22974
                     /locus_tag="ACHKTW_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001397936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFO6462848.1"
                     /translation="MTDINHDNTVYPSSSFSGSPYLYQQVCTSSPDTRLTQINLLSVA
                     KPQLVRRANGRYTIRFRLKGQTIPFLSVSTRSTDRRVATMRQRELAATAKSFMLDRPD
                     VSLQELTDHLRSMAEQFLTDASDDYWNGLEVATLVDEKSNLKELAATQALSLDQQKGI
                     RLALEVLTAAQQRVDTGDASGLIKLIDDNNHTDYSTIGDSTSILKNEQGDRPAVFTQE
                     RQPSVVFSSLVSSLLAEKVQTLKSSSYKDLSSSLNTVSRFLPEDMDLMSRSEWLAVRD
                     SMLAAEVRPSTINKLLTKAKMCLDYGLMNGQLEGRNPIERMKLTKDIDSKRRAFTDEE
                     LERLLVRVESEYQFTRHTAHTTSEARRWASLVSVVTGARSAEVCHLTKRDIVTLDNGL
                     TCIDINEDGDGKSVKNKHSVRLVPLTDGACGFDLKSFLEWVDTQPDDGGPLFGMTPSA
                     YSSWFNSRVLTEALGDAENVSLHSLRHWLATRMKERGVNLVDAQGILGHSSQSITYDL
                     YGKGHAVGRLAEVLKTALL"
     gene            23468..23836
                     /locus_tag="ACHKTW_001815"
     CDS             23468..23836
                     /locus_tag="ACHKTW_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6462849.1"
                     /translation="MARPIATHDNTFTKAYLQQHCGDLLSFDGQGDLSGWLDDVLTGA
                     GRLNESMASNTKPVSPYLILTQLLTHDTLTVSAVQESLSRKRVALGEPMVSTRYARYV
                     YATVVSASKSVQYHASKAGS"
     gene            24497..25255
                     /locus_tag="ACHKTW_001816"
     CDS             24497..25255
                     /locus_tag="ACHKTW_001816"
                     /inference="COORDINATES: protein motif:HMM:NF016283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005634 - nucleus [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HFO6462850.1"
                     /translation="MSTQPLMKHEVVLPVIVDGYHWACNQDRMWNLNELHKELELPSA
                     KEPGQWRSSVRTLLDQDANLHVAHGGSTPGTWATEEAAIAYAMWVSPDFYLKVIRAFI
                     ALRNDKVSEAKALAQDSKELKGLKPIARNWLEKLDNPKQGHTLTECLKNLDFRLGTKL
                     VSAKALNAVLKSGRIANPFYSRKLDGRGQPVFDISSGGWMTSFNPKGLENGYYRLQDN
                     HYNGQEGLKVLTKGYEWLKSNKVELVRLCAKGGL"
     gene            25521..26012
                     /locus_tag="ACHKTW_001817"
     CDS             25521..26012
                     /locus_tag="ACHKTW_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6462851.1"
                     /translation="MMFNNNNWKLSVTDINLYENTVSLDGQSYPLSLAIKTLIPGYLS
                     GLPSTSRESMELLEALAEAGVAIGNFFSNDLMTAYGRRQQNKRAEAERIAKEQRIQAE
                     RMREENMTDAEWQKELQRREQVKAERRTYGENLRSATHSAGRSRAAIVADLESGGNWL
                     DSL"
     gene            26094..27083
                     /locus_tag="ACHKTW_001818"
     CDS             26094..27083
                     /locus_tag="ACHKTW_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6462852.1"
                     /translation="MMTIKDAFQFGIEPVRITDTDNIQVNEGLPTNADPQVYALQLAK
                     TVKAMLNGVLKDAQENIPFPVEVLPTRNSLPTPIIAHTLTDRSVVVPVRGGKRPEVVT
                     VPSGQEIVVEPIEQAILISEQTKLWDAKSSTGFTQGTLQQDAMNICENVVRTINARMV
                     DVLESSKLLKTVELPVLTGSLTAKADAIMDALYENTESSFGSEVSDYGIIAHESQLKA
                     LSRLAAKQGFSGEDAIVDMLGTDIAYYNGEDKGVFMLAKRFTALSFGCFRHDGENITV
                     VLSRDGDSQSHDLEILGKVFVVAEAATTIKMGTGSATAVLPVVKRLKFTKTEA"
     gene            complement(27959..29371)
                     /locus_tag="ACHKTW_001819"
                     /pseudo
     CDS             complement(27959..29371)
                     /locus_tag="ACHKTW_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416104.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0009389 - dimethyl sulfoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DMSO/selenate family reductase complex A
                     subunit"
     gene            complement(29570..29875)
                     /gene="ynfD"
                     /locus_tag="ACHKTW_001820"
     CDS             complement(29570..29875)
                     /gene="ynfD"
                     /locus_tag="ACHKTW_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HFO6462853.1"
                     /translation="MKLSTCCAALLLALASPAVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYTRTTSGNVSAPAQSTQDGAPAEPQ"
     gene            29983..30693
                     /gene="ynfC"
                     /locus_tag="ACHKTW_001821"
     CDS             29983..30693
                     /gene="ynfC"
                     /locus_tag="ACHKTW_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HFO6462854.1"
                     /translation="MKYKLLPCLLAIFLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAET
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDSHANPVDCQLIIVDEG
                     VKPAVERVYTIKNTIDYY"
     gene            complement(30696..31256)
                     /gene="speG"
                     /locus_tag="ACHKTW_001822"
     CDS             complement(30696..31256)
                     /gene="speG"
                     /locus_tag="ACHKTW_001822"
                     /EC_number="2.3.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
                     /protein_id="HFO6462855.1"
                     /translation="MPSAHSVKLRPLEREDLRYVHQLDNNASVMRYWFEEPYEAFVEL
                     SDLYDKHIHDQSERRFVVECDGEKAGLVELVEINHVHRRAEFQIIISPEYQGKGLATR
                     AAKLAMDYGFTVLNLYKLYLIVDKENEKAIHIYRKLGFTVEGELMHEFFINGQYRNAI
                     RMCIFQHQYLAEHKTPGQTLLKPTAQ"
     gene            complement(31291..31632)
                     /gene="ynfB"
                     /locus_tag="ACHKTW_001823"
     CDS             complement(31291..31632)
                     /gene="ynfB"
                     /locus_tag="ACHKTW_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1283 family protein"
                     /protein_id="HFO6462856.1"
                     /translation="MKITLSKRIGLLAFLLPCALALSTTVHAETNKLVIESGDSAQSR
                     QHAAMEKEQWNDTRNLRQKVNKRTEKEWDKADAAFDNRDKCEQSANINAYWEPNTLRC
                     LDRRTGRVITP"
     gene            31767..32093
                     /gene="ynfA"
                     /locus_tag="ACHKTW_001824"
     CDS             31767..32093
                     /gene="ynfA"
                     /locus_tag="ACHKTW_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HFO6462857.1"
                     /translation="MIKATLLFFATALCEIIGCFLPWLWLKRNASIWLLLPAGISLAL
                     FVWLLTLHPAASGRVYAAYGGVYVCTALMWLRVVDGVKLTLYDWTGALIALCGMLIIV
                     AGWGRT"
     gene            32299..33513
                     /gene="rspA"
                     /locus_tag="ACHKTW_001825"
     CDS             32299..33513
                     /gene="rspA"
                     /locus_tag="ACHKTW_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="starvation-sensing protein RspA"
                     /protein_id="HFO6462858.1"
                     /translation="MKIVKAEVFVTCPGRNFVTLKITTEDGITGLGDATLNGRELSVA
                     SYLQDHLCPQLIGRDAHRIEDIWQFFYKGAYWRRGPVTMSAISAVDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGHSIDEALDDYARHQELGFKAIRVQCGIPGMKTTYG
                     MSKGKGLAYEPATKGQWPEEQLWSTEKYLDFMPKLFDAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRMFWMEDPTLAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTLTHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCMAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHNWTFDNGYMHPGDKPGLGIEFDEKLAAKYPYEPAYLP
                     VARLEDGTLWNW"
     gene            33525..34544
                     /gene="rspB"
                     /locus_tag="ACHKTW_001826"
     CDS             33525..34544
                     /gene="rspB"
                     /locus_tag="ACHKTW_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent oxidoreductase"
                     /protein_id="HFO6462859.1"
                     /translation="MKSILIEKPNQLAIVEREIPTPSAGEVRVKVKLAGICGSDSHIY
                     RGHNPFAKYPRVIGHEFFGVIDAVGDGVESARVGERVAVDPVVSCGHCYPCSIGKPNV
                     CTTLAVLGVHADGGFSEYAVVPAKNAWKIPEAVADQYAVMIEPFTIAANVTGHGQPTE
                     NDTVLVYGAGPIGLTIVQVLKGVYNVKNVIVADRIDERLEKAKESGADRAINNSQTPL
                     GEIFAEKGIKPTLIIDAACHPSILKEAVTLASPAARIVLMGFSSEPSEVIQQGITGKE
                     LSIFSSRLNANKFPVVIDWLSKGLIKPEKLITHTFDFQHVADAISLFEQDQKHCCKVL
                     LTFSE"
     gene            complement(34732..36012)
                     /locus_tag="ACHKTW_001827"
     CDS             complement(34732..36012)
                     /locus_tag="ACHKTW_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3596 domain-containing protein"
                     /protein_id="HFO6462860.1"
                     /translation="MKYPTGVENHGGKLRIWFVYKGVRVRENLGVPDTAKNRRVAGEL
                     RSSVCYAIKTGVFDYAKQFPSSRNLEKFGEARQDLTIKELAEKFLALKETEVAKTSLN
                     TYRAVIKNILSIIGEKNLASSINKEKLLEVRKELLTGYQIPKSNYIVTQPGRSAVTVN
                     NYMTNLNAVFQFGVDNGYLADNPFKGISPLKESRTIPDPLSREEFIRLIDACRNQQAK
                     NLWCVSVYTGVRPGELCALGWEDIDLKNGTMMIRRNLAKDRFTVPKTQAGTNRAIHLI
                     KPAIDALRSQMTLTRLSKEHIIDVHLREYGRTEKQKCTFVFQPEVSARVKNYGDHFTV
                     DSIRQMWDAAIKRAGLRHRKSYQSRHTYACWSLTAGANPAFIANQMGHADAQMVFQVY
                     GKWMSENNNAQVALLNTQLSEFAPTMPHNESMKN"
     gene            complement(36047..36298)
                     /locus_tag="ACHKTW_001828"
     CDS             complement(36047..36298)
                     /locus_tag="ACHKTW_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000005552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HFO6462861.1"
                     /translation="MSEVIMIVSPGKWVSEEQLIALKGIKKGTLKKAREKSFMEGREY
                     KHVAHDGMPWDNSPCFYNLEEIDRWIERQASARPRCHLT"
     gene            complement(36371..>36481)
                     /locus_tag="ACHKTW_001829"
     CDS             complement(36371..>36481)
                     /locus_tag="ACHKTW_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000528968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HFO6462862.1"
                     /translation="AIPFEGERHNALDDARYQAKYVSVIWQKLIPSQADS"
BASE COUNT         9030 a         8648 c         9636 g         9167 t
ORIGIN      
        1 ttattaaaaa gtttagcgtg ttatctgaat acaggatatc ttaaatgaag agtatagcaa
       61 cactggttgt gtgtgcaatc tccgggattg cctgtgtaaa tttatctgca catgcagcag
      121 aaggaaacca tacaatttct ctggggtatg cgcactttca gtttccggga ctgaaggatt
      181 ttgtaaagga tgcgactgct cataacaggg agactttcag tcatttcgtc aacagaaact
      241 acttttcttc attgggcgaa tatacagatg gtcgggtcag tggatatgaa ggcaaggata
      301 aaaatccaca gggcattaat atcaggtatc gctacgagat aacggatgat tttggcgtta
      361 tcacctcttt tacatggacg cgttctctca ctaactcaca gacatttatt gatgtgcagt
      421 cagccgatca taccaggaag attaagaatc cggcagcttc tgccagaacg gatatcaggg
      481 cgaattactg gagtctgtta gcggggcctt catggcgggt taatcagtac atgagcttat
      541 atgcgatggc agggatgggc gttgctaaag ttagcgctga cctgaaaatt aaggacaata
      601 ttaacagtag tggcggattt tctgaaagca acagcacgaa aaaaaactcc cttgcgtggg
      661 ctgcaggtgc acagtttaac ctgaatgaga gtgttacact ggatgtggct tacgaaggtt
      721 ccggctctgg cgactggcgc acgagtggaa ttactgctgg cattggcctg aaattctaac
      781 ctgtatccgg taaccgttta ctacccgctg tgatggcggg ttttttattg cccgtacagg
      841 gcaaaaaccg taaattatgc gtgggtgcct ttcggctgat ggctggaggg tgaacctgaa
      901 ggcctgatgt ggaaaggccc cgagtcaact taacgttaac ccgaggccct aacacttcgt
      961 accttgacaa gtagaaggtt agcgcctctc cactcaggag gcaagcgcta tgtcgcaaaa
     1021 atcgcttatc accgtcacaa tttgtatgac ggttatcttc actatctgga tgttgcacgg
     1081 ttcgctgtgt gagttccggc tgaatttgtg gggagcggag tttgcggcgt tcttacagtg
     1141 taagcagtag gaaaaccgcg acggggacga aagtccccgt caactggttg ctgaggttca
     1201 gccgatatgg cacccgtttc aggtgagaga atgaacgata aaattctccg gtatatgcag
     1261 cgtgtggtga gaaattcccg caaccctgaa tttatgaatg aagttaaaga cgcctgcctt
     1321 aaaaagcagg cgttttgttt tgaggcacct gatggctttc tggtgctgcg ttctgtgctc
     1381 agtgctgatg gtatccctta tgttctggtg ttgctgggcg tgtgtacggg gagtaacagc
     1441 gttgagcgtt atctgccgga ggtgaagaca ttaacccgtc tggctggcgg acgttgggct
     1501 gaatttcata cggcaaggcg gggatttatc cggctgggaa aacgtctggg ctttgagcga
     1561 atgccggatg atgaggatgg cttcatggtg ttcaggatag cggtctgact gccacagttt
     1621 tcatcatcgt gtttaaacca acattgcaat tcacattctg accctgctcc ggcagggttt
     1681 ttttattatc cagggggcca ttatgggtgg aagtaaaggt ggtggtgata ccaaagtaaa
     1741 accaacagca gcgcaaatag cacaggaaga agtggcctgg aaagggtggc aggattacaa
     1801 aaatatcctc cgcccggctg aagataactt catggaaaag gtcgatgacc tgaacagtga
     1861 gcagcagtac gacaatatcg ctggcaccac aaatctgggg tatcagaaac agtttggcga
     1921 agcacggaag gagctggcgg gtaatcttgc tcagtccggt gttgacccgt ccagtggtcg
     1981 ctttaacgcg gtaatgaatg cgaaccagag tgatcaggta accgggcaga ttgacacaac
     2041 cacacggggg caggtatcgc aggcagataa gtatgttgcc gggctacagg atgttgcagc
     2101 tctcggttca ggtcagaagg cggatgcgtt acagagtttt aactcgctgg cagacagcag
     2161 tctggcaaaa gctaaatcgg acgcacaggc tgcgtttacg aaacagcagg ggcgagcctc
     2221 tcttgttggc gctggtttgg gtgcggtagg tgcatatgcg atgcataagg ctggtggtag
     2281 cggaggaagt ggcggtgcta aaacaccggg caccggcgct aatgccattc agcatcaggc
     2341 tcagaactgg agactgtgat tatggagtac ggtaaatacg aaacacttgc gagatacggt
     2401 tataccggag ctgcccgtcc acagggtgac tggcagacgt cagcagcgct gacacgccaa
     2461 caatacgacg actggcgaac cagatatttg ccccgcgtgg caaggctggc tgaccttggc
     2521 gagaacaaca gcctgatgaa tgcacagctt gcccgggtgg gcggccttgc cacttccagt
     2581 ctccgtacag cgcagatggc gcaggataac cagatggcga gatacggggt aagccgcccg
     2641 gataatcccg acagtaatac gctggggtta cgtaatgccc tggcaattgc tggcgcgaaa
     2701 aatggtatcc gtgaagccga acaggatcgc cagatgaaca tactgacggg ggcttctgca
     2761 ccggcaagac agaaactgag tgttggcgga caactggtgg cagcgtaagg aggaaatatg
     2821 gggtacggct tactggatat tgcgaatcag tcacggcgtg aggcattaca gggaataagt
     2881 gacgcagacc gacgacgtga agaaattgag gctgcgaaca aacagatggc ggcgcaacag
     2941 aaagcgcaga acaagcagaa tatcggtacg ggcattggta cgggggcggc tattggcgca
     3001 tccgttggtg gtcctgttgg tgctgttgct ggagcagtaa ttggcggcat tgctggttct
     3061 ttgttttaag gagtggtgaa tgagcggatt tgcacagggg ttacttgccg gattcagcac
     3121 ggttgaccag gcaatgaccc gtcgtaagga gcttggtctg cgtgaagcac agcttgcccg
     3181 gcaacagaaa aataacgagc gcgattttga gtttgcgcag tctcagtttg aacataataa
     3241 aaacgttgat cagcggaact ttgattacag agccaaagtt gacgaccgta attatgcact
     3301 gaaggaaagg gagtttaacg ctaaccagaa ttaccggaat gcgtcgctgg gtatggagca
     3361 gcagcgactc cggatgcaga aatacaacca gcgacggctt gagtataacg atatgctggc
     3421 gcgcgatcag cctgtgatgg cggcgcttgg aaaggctgtg gatgctggcg atcgggatgc
     3481 agcaatgcgt ctttacggcc agttgtcaga gggtaatccg ctgaggctga tggcgaatga
     3541 tggctatgta gcgaaagcgg gtcaggccgt gaacaacctg caaaaaatct ttgatgacaa
     3601 gccagacagg gctatcgctt cacttaatac gccggagaat cttgatgtcc tttccggggt
     3661 gtttggcccg gaactgcaac agcgtattgg catgcccgat tcaaccggga aaaagacgat
     3721 aaaagaggcc aggattggca gtatagtacc ggcacagcag gaagggtaca tacttatcgg
     3781 ccttgatctc acatacagtg atggctctac agcacataaa cctgtaacag aatacggcag
     3841 tgcgcaccct gatgatcaaa ccgtgctggc gatacccgtt gataaggcta tcgctcaggt
     3901 cagggatcgc agcaaatttg cagagatatc gaaaaattat ggttatttta tgccgaagca
     3961 gcagggactt tctctgaaag agcttcagaa gggggccagc aacgtcgcgg cggacgcgat
     4021 caagaatggc ggtaatgcac aggctgcggt ggatgaatat tatgctgcga ctggttcaca
     4081 accgcatcaa cagaaaattc agcaacaaaa acttcagcaa caggttatca actgggcggg
     4141 agatgatcct gataagctgt catttgccag aaatgtagcg gcccgtcagc ctgaaatgct
     4201 ggagcctcag aatcagaaat tgctggagaa cgggtatgcg aattttctcc gtattcaaaa
     4261 ggccaggggg gaacaggcca gagatgaaag tgcttcatct gcatctcagt ttatccgtgg
     4321 actgaaacag aattacgccc agtaattcac gatattccat taataccatt tcctgatgcc
     4381 cggccattgt gccgggtttt tttatggagt ctgtatggcc tattcagagg aacagcgtcc
     4441 tgaggcgcaa ctcggtaacc agaatcgtaa cagcctgaac attcagcaac ccggcgaaac
     4501 tgacagctat gaagcatttt tctctgatcc gaatcgctgg aaggataaca gtacgtcgtt
     4561 cagcctgggc gatgtattgc caacaatggg taaaggtttc gcccagtccg tccggggaac
     4621 aggggaaatg gcccgtggac tcggtgatgc gatgattcag agcccggtaa aaacaggggc
     4681 gcgtatttta aatgagttca gccgtatggg gctgccgggt gtcgcaactg tgcaggatat
     4741 ttttgccggt ggcagcaggg gggctgatga ggtcatcgat accctgcctg atggcaaaaa
     4801 cgcggttact gatactgtcg gtaaaggtct gaaggcaacc ggtaaggctg tcagtgatgg
     4861 tgctgaagcc actgatgaat ggctgaccgg taagatgtcg ccgggtgcag ttcgtgcgct
     4921 gaatacgccg atgaccgaag gctatgatga ttctgcggtc tgggtggcga agggtgtaaa
     4981 cctgattggt gcgcttgtac ctgatatggt tgctggcggt gtggctaaaa aggtgggtga
     5041 tgtcacactg cgaaaaatgc tgaccgccgg gctggagaaa aaatacatcg cggcagggat
     5101 gcagccggaa agagccacgg cactggcagc agaagctgtc gataaaaaaa tgccggattt
     5161 attccaggcg ggcctgatca cccattccac agccagtgca caggggcaga gtgcaatggc
     5221 ggcagcagat gctgttctta atgcggatta ctctgagctg gcgcagtcac cgaaatttca
     5281 gcagacgttt ttgtccattg acgccgaccc gcagcacgca cagcttactg atcgccagaa
     5341 aatggatctg gcaaaagagc gtgttgccga tgaggtgcgc gcgcagctgg caaccgatcc
     5401 tgaattgctg gctgtgaatg ccatggcggc aaaactgggt gacgcacaac tgtttaatct
     5461 ggtgacacga ggcacagcga agaccgttaa aagcggcatt gtcagaaatg ccacggaaca
     5521 gggggcgatt aatgcggcgc agggcggcta ttcacgctat caggaaaaca cggcattgcg
     5581 tgagaccgcc ggaatggatg tgtcaccgtg ggagggcgtg gctgacgcaa cgatcgaagg
     5641 tgcagccttt ggtgctgcga tgggggctcc atccggtgcg gttgccggat atcgtggcag
     5701 acgtcaggcc gcagaagaag cagccatgcg tgatgctgaa accgtgcagc aggacgacgc
     5761 agccccgcaa ccagaatctg ttgatccggt ggcgcagcat cgtgaatcca tgcaggggat
     5821 gaatcgcgag cagcttctgg agcagtatgc tgatgcggat atggcaacag agggggacgc
     5881 atccgcagct catcgccggg aagctgccag ccagttgttg aatgaactgg acgaacaggc
     5941 gaagcgacag gctgtgatga atgagctgaa ggcgaagccg cgttctgaac tgcttgagga
     6001 ataccgcaga ctcagccaga aagaggagcg caccgagact gaagaacaac agtttcaggc
     6061 aatacgagaa gtcattcgcc cacaacagga agtgacgccg gaagcacagt cacagcctga
     6121 aaatgcggag gatggtaacg ggagcattta cccgacggtg cggttccggg acccgaatga
     6181 agtccgcatt gaaattaacg ggaatggtgc gtccagacca gcggaacgca ttgaaaaggt
     6241 gcgcccggac aaccgttatt tcacggatga gaaaagcgcc atggggagtg atgttttccg
     6301 taatgccgcc gccaccggcc tgaaaccgtc cgtagtgaag aaaggcgaga ctcaatatgc
     6361 tgttgaaatg gataatcctg cgttctctga agatgtggca acggaaacca ttaacaccct
     6421 ggctgacgga gagcgtattg ctgatgctga cccgatggag cagcccgcgt tcatgcgtga
     6481 cccacgattc cgtggtttca cgggggatga tacggaggta caggcccgcc ttgcccgtgg
     6541 caacgtgccg acggcagagg agcttgtacg ttcacagatg gctgaaggtg atgccggtcc
     6601 gacagcacag gagttaactg agcgtccacg cctgcccgct cccggcgata ttcatcccgg
     6661 acagggatat ccgttaccgg gagaggtggc gcgtacgccg gatgagaatc aggcaggacg
     6721 tggtggtcgt tttaccacaa ccggtgaggt taagggccag agtttccaga aaggacaagc
     6781 tctggcaccg gaaaacgccg ctggtcgcca gggggaaaca ctcgagggtg aaatggttcg
     6841 tcgtggtctg ccgtcaccgg atgcgcagaa cgcgacagca ccggtacgtg aagggctacc
     6901 ggctccggac attacacaca atgtccgtat gcctcagcct gatcagcttc cccgaactgt
     6961 tcgtaactca ttgcctgagc tcgcacagca ggcagaagta cgtcgacagg ccggaggaaa
     7021 tcgtgacatc ccgcagcctg agacaatcgc acctgaatct gaaacaacag tcgctactga
     7081 cagggaagct accgtgcgcg gaggtgaagt caggggcaaa aaaattgaag actttggtga
     7141 agaaattaag ggcgcggcaa aacaccgtta tgcacagctt gctgaaacac tgggtaaaac
     7201 gctggaagac agggattatg tcacgcaacc gctgagcaaa ctgttcccga aaccggacta
     7261 cgcaaaactg gcgagcgaag gggctgatgc tgacaccctg gcaatgatag cgctgtatcg
     7321 tagcgatatt ccggtgaaga cgaaacacaa tacggcaggc tggggggaga gcgtaaaaaa
     7381 agtacgacac agtgtatcgg aaatgctgaa cggaacggtc agcgcgaaac gcctcgcaga
     7441 atggatggaa ggcagaatgc cctcccgtta cgcggacacc tggcaactgt tacgcactct
     7501 gccaccctca cagatggaca aggcttctgc ttatcgggtg gtatcgggtg tgtatcaggc
     7561 ggcaggaggg aagcgttacg atccgccaca gaaactttat tcactgcgca ataaggacaa
     7621 taaggggagt aacctctttt tctcggaaag cagggatgaa ttactggcaa aggcgaaagt
     7681 ctggtttgca gagcaggagg aaaaatcaca ggcgaaaggt gatgaaaaaa cagcaccgtc
     7741 tccggatgac aaaatccgct ttgacgttta ccggaatacc cgcagtggcg atatttttat
     7801 cgcttacggt aaaaacaaaa tgcgggtgag aggtggcttt aagtcagcca gtgatgcgcg
     7861 taagtacatt gatttacatc gtgatgagct tgttcgtcat gtgaaggaga tgcgggagat
     7921 ttcgcgtgag gagcagcgca acgccaccaa ccgcgaccgt accggaccag aacgccgcaa
     7981 gggggatgtt tcaccggagc agttcagtga tgcgtttggt tttcgtggtg tgcagtttgg
     8041 taattatgtg gaaagtccgc gtcgtcaggc tgatttgaac cgggcttatg actcgctgca
     8101 tgacctggct gacgtgctga atgtaccgac aaaagcgctt tctctgaacg gtcgtcttgg
     8161 gcttgctttt ggtgcccgtg gtaagggtaa ggcggcggca cactatgagt caggtgaggt
     8221 ggcaatcaac ctgacaaaag gtaacggacc gggtgcgctg gcgcacgaat ggttccattc
     8281 tctggataat tattttggtc gttatgacgt ttccagtgac gggaaaatta cgtcaggtgg
     8341 cgactttatg acggaagcac agcgtgtcag gcgcatattt aaagacggca ggtatgttga
     8401 tgctgaatat ccggtacgtc aggaggttta cgacgctttt aaaggtgtga ttcaggccat
     8461 taaaaacagt gacatgccgc gtcgttcagc gcttcttgat gaggtgcgct caaaaccgta
     8521 ctggtcaacg gatgttgaaa tggcggcacg tgcctttgag cgttatgttc aggataaggc
     8581 gcgtatggct ggcgtggaga atgattatct ggtcaatatc cgtaaggcac ctgagcacaa
     8641 cacagataac acctacgctt atccgacgaa tgcggaactg gatggcggta ttcgtgaggc
     8701 attcgatcac ctgttccgca ccctgaaaac ccgtgagacg gacaagggcg ttgcgtttta
     8761 ttcccgtaag ggcatgaccc gcacacctga aggtaatctc atttcggatg ttaaccgtag
     8821 tgcggaagcc aaaggcagcc cggtcccgca ggttgaagcg gttgcccgtg gcgtgatgag
     8881 cggcattaag gacagtgacc tgacggtccg tgtggtgaag tcacagaaag aggctgaagc
     8941 gctggcgggt gaatcgttcg acggttacgg cagggtgcac gcattctatc gtccggataa
     9001 acgagaaatt gtcctggtgg cggataacat ccctgacggg cagaccgtac gcgagaagtt
     9061 gcgtcacgag attatccacc atgccatgga gcatgttgtc acgccagcgg aatatcagac
     9121 gattatcaaa accgtgctga aaacccgcga cagtgataac gccaccatcc gtgaagcctg
     9181 gcgtaaggtt gatgcgtcct atggtaagga atcaccggaa gtacaggcgg gtgaatttct
     9241 ggcacatatg gcggagaaac agccgaataa attcgtggcg gcatgggagc gtgttgttgc
     9301 cctggtcaaa ggggtactgc gtcgtacggg gttactgaag ccgacggaac tgaacgatat
     9361 cagacttgtt cgcgagacca tccgtacgtt aggccagcgt gtgcgggaag gttacacgcc
     9421 gcgtgaggat ggcgtgggcg catcgtctca gtactcccgt agtggtaagc ctgatccgtt
     9481 caaagtgccg gaaggtgagg gtgaacgtta tcgcgatgac cttgccagaa tgatgaaatc
     9541 tctgcgtacc acagatttaa cggtaaacat cgggcgtacg ccgccggtat tgcgtcacct
     9601 tggtgcaccg gatttgccgc tggttatttc ccgcgatact gtgcggaagg ccaccaatgg
     9661 tgtgaaacat gtggtgccga tggatgttat cgagagacta ccggaactga tgcacgatcc
     9721 ggatgcaatt taccgctcag cgacagaaag aaatgcggtt gtgatgctgc ttgatgccgt
     9781 ggataaaaat ggtgatccgg tagtgtctgc ggtgcatatg aaggcaaccc aaaaacttct
     9841 ggaagttaac cgtattgctt ctgtttacgg aacggaaaac gggaaaaaac tgaagagtat
     9901 ggaaacggct ggcttaacat tatacaggag agaaaaacta aaccctgatg gttctctgta
     9961 cagagggctc caattgccca aagatgagca ctcccgtcag ggttctgcgg ataaaatact
    10021 ctatcctgaa gatattcgca aggggccgta ttactcccgt accagcagtc tgacaccgga
    10081 agagacaatt gcatcgcgtt ttgtgcgcca gatgcaggat aaattccagg tgctgaaagc
    10141 tgttcaggag aatatccgta aaactggtgg aaaaatagac gacagtaaca acgcttatat
    10201 ggcggaagaa ctcttccacg ggaaggcgga aaacgacctg aacgtgatga aggagcgcta
    10261 cgttcagcca ctggctaaat tactggcgga ctacaaaatt gcgcaggccg atctggatga
    10321 gtacctctac gcccgtcacg cgccggaacg taacgcgcat atcgcgaaaa tcaacccgaa
    10381 aatgccggac ggcggttcgg ggatgaccaa cgcggaggcg gcggaaatca tgcagcgtgt
    10441 acgtaacagc ggcaaacagg cacagtatga ccgtctggca gggattgttg acgatatgct
    10501 ggcccgtcgc cgtgagctta tccgtgaggc aggactggaa gagagcggcg tggtggatgc
    10561 ctggcagaaa gcctaccgtt actacgttcc cctgaaaggg caggatgttg acggtgtggt
    10621 gtcactgcca cgtacaggca agggattcac catcggcggg cgtgaaagta agcaggccat
    10681 ggggcgcgca tcccgcgcac agtctccttc cactcaggcg atacaggatt tgagcgaatc
    10741 actgatccgc aatcgcaaaa atgaagtggg taacgccttc ctgaaactgg tacaggataa
    10801 tcccgacaag gattactggc aggtattcac cgatgacaga ccggatacca tgcggacgat
    10861 tgcagagcgc aaggacaagg aaactggtga aaccattcgc gaagttgtcg aacgccctgt
    10921 accgatggca atgatggcag accggtactt caccaccaaa aagaacggca aaacgtacta
    10981 catcaaactc catgatccgc gcctgatgcg tgcgatgaag aatatgggac cggaaaccag
    11041 taacgccgta atccgcacgc tggggaaagt taaccgcttc ctggcaacgg tgaacacgtc
    11101 gtataacccg gaattcctgg tcagtaactt catccgtgac gtacagacgg cggtgatgaa
    11161 cctgaaggcg gagcagggaa ggagcgacgg caaactgaaa gggctggata acttatccgc
    11221 actggctgtg gtgaaagaca gtcggtctgc catgtctgcc gtatacgcca gtctgcgtgg
    11281 taaaaccctc acgggcaacg gtgcgcagtg gcagaaggtg tggaaagagt ttgttgagga
    11341 cggaggtaaa accggctggt ttaacatggg tgaccttgaa ggccagcaga aggaaatgga
    11401 tcgccttgta tcgctggcga agggaggatg gaaaggccag agtatcggtg catggaattc
    11461 gttccttaac cttgtcgagg atgccaacgg ggcggttgaa aacgctctgc gtctttctgc
    11521 ctataaacac gcccgtgatg ccggtttgtc acgccagcag gcggcgtctc ttgccaaaaa
    11581 catgacggtg aactttaacc gtcgtggtga gcagggggcg ctgatgaact cgctgtacat
    11641 gttcgccaac gccagtattc agggaacggc aaatctggtg agaacgctcg gacatcttaa
    11701 tggtgaggga cctttactgg agcgccttcg ctggaagaat ctgaatgttc cgcagaaaat
    11761 cgcgcttgcc gctgtgggag cgggttatct gcttggctcg cttaaccgca gcgtggcggg
    11821 tgaggatgat gacggggtta actggtatga caaagtaccg tctcatgtga aagagcgtaa
    11881 cctcgtcatt atgaaatcgg tgttcggggg caaggccgga gagtactgga gtattcctct
    11941 gccttacggg tacaacgttt tcttcctgct tggtcacaca gccgaagggg tggcagcggg
    12001 cgacctgacc gcttcacgtg ctgccggtaa tgttgttggt ggtgtgcttg gggcattcag
    12061 tcctgtgggc agtgaaacgt cggaaacact gtccggggca ttgctgaaaa atgcagcgcc
    12121 gaccattctg cgtccgtttg cgaaccttgc catgaatgaa aacttcatgg gggcgcagat
    12181 ttaccaggag aacatgccgt ttggtacacc aaaacctgac agccagctgg gaagacgttc
    12241 aacgccagaa gcgtacaagg cgtttgcatc ctggctgaat gcgttctcag gtggcagcca
    12301 gtaccgtccc ggcgcggtgg atatcacacc ggaatcgctg aaattctgga ttgactatat
    12361 ctccggaggg acagggcgct tcatttccaa aaccacggat gcggcggtga aatcgctgaa
    12421 tggtattgat ataccggaac agcaggtgcc cttcctgggg aaaatttcgg gggaggtgat
    12481 gccgtatgca gaccagcaga agatgtacga ccggatgaca gagattgcgc agtatcacgc
    12541 agagctgaag agtctgaccg gtgcagaaag aacggcgttc attgacgaga acaacggaaa
    12601 attgtcgatg aacgggctta tgcaggatac ccggaagaga ctgaaggatt tgcgtaaaca
    12661 gcgtgatgcc atttatgccg acagttctct cagtctggcg cagcaggcgg cgatagtgaa
    12721 atcggtagag cgggatatga aggttgccgt ggatcgcttt aaccgcgagt acaacaaaaa
    12781 agtgggagtg gattaacaga aatggccccg tacggaagtg cggggctgat taaggaataa
    12841 acacacatta acctgtaata accggagcta ttaacatata gtcagaaaga gtatttcatg
    12901 tgagacagag agccgattta tgtttaatga agaaaaagtt gcgcaaatgg cagcgtattt
    12961 gctgaaaaag catggcggat ctatgcgttt cattaagctg atgaagctga tgtatctctc
    13021 tgaccgcaaa gcaatggagt ctttaaccgg gaaggggaga ggtttgaatg ctcccccaca
    13081 attccccggc atcaaatctc catgcaggtg aactatttta cccccagcgg caaatcgcaa
    13141 aaacaatcag tgcgatcgaa atggcagcca ctacaattgc aaatgcttca ggctaggtca
    13201 ttggcttacc tccttcggcg gttctggtag cggcatccag tgggttactt ctttgagatg
    13261 caggtcttcg ccatcaccgt catcccaagt gggattgcca tcattaaacc agtcgccata
    13321 tacgccgacc tgagtgttgg ggatgcttgg tgggtagttg tttttaaagt cagctgctaa
    13381 cacatagtat tgtcgctctc ccatttctgg cattcgatca ctacagctta tccaaccatc
    13441 cggagttacc ggaactggcg gaacggctgt ttgctctcga acgtcattag tcgctatcgg
    13501 ttctgctgcc aactgactgg catatttgtt aatggtaacg ataagctctt gctcagcctc
    13561 atccagacaa tcaccgatac ctcgcctgtc accgtcaaaa tcatcgaaat cggcacgaat
    13621 cttggcaacc ttctggattg cggacaacac ctcactagga attaccggat agttggttga
    13681 cgtttccgcg atttcccgaa aattattggt tgacgaattc ttgttttccc gaaagtttcc
    13741 ggactgaagc atggcggcgc ggcaggcatt ccagcctctt acctctgcaa tagcggcaac
    13801 cgcatcaacc gcgtacatgc taagaggatt aggcattggt ttttcttccg gtactactgg
    13861 tgctggaggg gcggcgtaaa tgccctctat cactaaatgt ttgcgctcaa aatcatctgg
    13921 ctctcgatga tatacgtaac tccaatcacc aaggttatca ttgcgcctgc aacggaaacc
    13981 tatcggctcg gcttccagcg ctgccagagc aattcgtgcc agttcttccg cttcttctgc
    14041 tggcagtaca acgttgctac caggtccgta tgtttcgcgc cactgcttga ttgtcagtag
    14101 tcgctctttg gtaatagtga tcatgccgcg tttccttctt tcttattaac aattacaccg
    14161 tcatatattt cattaaggtg ccctctcaac tccatgcgcc ttaatgcaga taacatgtaa
    14221 tcgcattcaa cctgcttatt cccaataaaa ggtttatctt cagggttacc ccaacagcaa
    14281 ttccccttgg gccatccatg tactttccgt actcttccgt taacaacgtg aagtaatccc
    14341 cagccaggtg gtaaatcctc aattgaaata attcccggct cactaataaa gaatcgccag
    14401 tcgcccattc caagagacgg atttttacga aaacgctttt ttctatctgc caacaagtca
    14461 gcacgagaac atttcgcctc tatcaggcat gatgctgaat ttctgaatcc catagcatct
    14521 ggctgttctc cggtactggt tacagctata aagcggtcat gaaaacaaac cttgaacccg
    14581 ttgcgcttaa ggaacttgta cgcaatctga cagagttcgc ggtgtgttaa cgccatatca
    14641 ctctcctttg atgcgaatgc cagcaagcca gtttcttatg ccgatatatt cagcgttccg
    14701 gaaaccgctt tttacatata taaatggcaa gcgaagattg tgaccattga ctgccaggta
    14761 gtctttacaa ccctgttcgg tgaaacagca ggtaacgaat tcatcaatat ctttcacagc
    14821 aacgcgccgc catttttctg gtggctctcg aaagttttca tgaagtagct cgagacgacg
    14881 actatggcgt ttattggctt cattgccatc ttcgtcaacc cagacaatcc ggtcatggtc
    14941 ataatcagca tcaacaacga tttcgcgctt ttgatacaca caaaacatgg gatctgacgt
    15001 tattcgattg tcctgtgttc gaatattttc accgatgatg ccaaacgaat ctggtgcaga
    15061 ttttgtctgc atctcttcga tacgttcagc catcgcagca cactcttcaa agttgcttaa
    15121 tgcttttcgc tcccattcgg cgcattgttt ttccagttct gctatgcgct tacttccatc
    15181 cgcgattact ccctcgtaat attcacgctg ctcgttgagt tttgattttg ctgtttcaag
    15241 ctcaacgcgc agcttcccta ccgtaagcgc aatatcctcg ttctcctggt cgcggcgttt
    15301 gatgtattgc tggtttcttt cccgttcatc cagcagtgcc agcacggtag ccggattggc
    15361 tgcggcgatg aattcagcat tggcctgctg ttccatttgg aaatcttcat cgaaaccgct
    15421 ttcaggatgt gctccttcaa ttctgcaaat gggaatatat ccagcagcct cgcgatgaat
    15481 tagtgcatca tcaccatcaa atcggttctc tccatattcg agcgaccact caccacgcgt
    15541 tgctttttct gccttttcac gcagtacctg atagttaatc tcgctcattt ttctcttcgc
    15601 tccggtatac aagaattaca acgtcacctc tgctaattac gcgagctgga tctcctggct
    15661 ccatgctgtc aatcccgaag gcttcggaaa acgcattcat tgccttctgg cgttgatcct
    15721 gcttacggca tttattccat tttttcagta acaacagcga tagccaccgc ccggcgcaga
    15781 acataatgta aaaataacca agaagcgcca gacctgtgtt gagggccgta tcgatcgtta
    15841 tagtggtgtc tatgctcact tcacacctct ctgtttgttg ataagttcaa tatcctgctg
    15901 gcaactggcg caagttcggc atccgcgaac agcctggcgt cgccgctcat ctatcggatc
    15961 gccacactcg caacaatgag tagcagatgg ggcattactt tcggatttgt atttttgcag
    16021 ggagagattg cgctgcaatt cttcgatttc agcggcattg tcgatgatat ctgccatttt
    16081 cctttccttc aggcatgaaa aaaggagccg aagctccttt ggtatcagaa ttcaaattgt
    16141 cttgctctta gttgcgctag catacttctg gccctatgca ccatatagtt ggcgggatca
    16201 agttttgctg cttcgcggag caatgtgtcg cgagtccggt tggttatatg acgagtctcg
    16261 taggccagat caaacagttt cccgtagtaa tcagaattca gttctctcat gacggggtat
    16321 agctgcttac tgagttgttg tgctttttcc atccagagtt gaacgtagca gagcaggatg
    16381 atgtcttctg ctgtgaattg ctgctcagtt tgtactggct gaatgttgcg aagtttcttt
    16441 tcgcactcga tgaagtatcg gcgtatctgg cggccttttt cgttacgttc aaccatcgca
    16501 agctctttgg ctgtgtcgag ggtgaggtgg taatccttgc ggcggcggcc tggagttttc
    16561 gccaaatttg gcgaaaatag aatgtagtct aaattttcta cgaatccata ctcgtttagc
    16621 ttggcccgta tccagttaga aaaatcttta cctacctcta aaaatccatg caaatcacgc
    16681 gcgttcacca gaagcgtagg ttcatttgag attgtacctt caaacacagg gatgagttga
    16741 gtggtcatga tgacctcctt tggctttttt cgagaaatgc caccacaaaa tgtggtgccg
    16801 ggaggctcga aacggcccaa aagataccgc ggacttattc cccttgcggg tgttgtattc
    16861 gtcgccctcc cgacattgat cggggatgtg accgcacact gtgctatcac tgaataacag
    16921 gcataaaaaa tccaacactg acggggttgg tttaatccgc ttttgggagg ttttcgaggc
    16981 ctcggcgcgg agtatagtca gtattggacg cagtcgtcaa ccatcgccgt ttctttggct
    17041 gtgtcgaggg tgaggtggta atccttgcgg ttgtgaccgc ctctaccaga tgtttgcttt
    17101 cccaaattgg aaagcaaaat atagtcttga ttttcaatga attcgtattc tgaaatgcga
    17161 tttgtaatcc atgccgcaaa cacctttttc acgcctaaaa aagcatgcag atcgcgggca
    17221 ttgcagagta gggctgtttc gttgtcgata gtgccgttga atacggggat gagttgatta
    17281 gccataatta tgactcctga cgttttagta ttgactgcca ccttagtggg gtggcgggct
    17341 tcaactaccg cgtcagacgg cggagcgtat ttcccgaaag ggtattgtat taggctctct
    17401 cgacccgcca ttgatatggc gatacctgta aacaggcata aaaaagccgc aaagctatcg
    17461 ggtgcggttg accgctgacg ttgtagtgca gtcagtatgc gatagctctg gcagatttgt
    17521 caaatcgtgc agtaacatcc ttttcttcct tgccatttct caatgatgac aaagggtgga
    17581 ttcggattgg tattgggaca aaagtgagac acacaaagct ttgcatcggc ttacaaagct
    17641 ttgcatgttt ttcaatgttg ggacgtgtga gcgcagaaat gacgggctat ctaattgatt
    17701 ttaaacgata cgtaaccaac tttaaaatct ttgcacgcca gttcgcaggt tttacagcca
    17761 gtacagcggc tggaatcgat aaaaaatcca tattgtgtgg tcatgggcta ctccttaaac
    17821 cttttcgatc tggacaagat tgctgtgcga cgggtttccc tttgccagcg gtgaagggcg
    17881 gtgagaggtc agaatattga tactgccgcc gtgatcgacc cggtcaccaa acatatccgc
    17941 tttaagccac gcaccttgcc cgatggcggt aacgccaggc agaatacgcg gagtcacttt
    18001 tgcggcaatc agcatttctc cattattgtt aaatacccgc acggtatcgc catgacggat
    18061 accgcgtgcc tgagcatcaa tggggttgat ccacacctct tgtgggcagg cctgctgtaa
    18121 cacatcaata ttgccgtagc tggagtgggt acgcgctttg tagtggaagc ccgttaactg
    18181 cagtggatag gttttccgca ggggatcgtc ccagccatca aaacctgggg tatacgcagg
    18241 aaggggatga ataatttcat cttttttcaa ttcccaggta tctgcaatct tcgccagtcg
    18301 ttcagaataa atttcgattt tccccgaagg tgttttcaac gggtttgcct gtggatcttc
    18361 acggaatgcg cggaaagcga cgtagtgttc ttccgggcat tttttcttaa agatcccggt
    18421 cgttttcatc tcctcgtagt cgggcatctc agggttacgt tccttcgttt tcgcatggag
    18481 atatttgatc cattcatgct gactgcgacc ttcagtaaag gtttgataaa cgtctggtcc
    18541 taagcgtttg gcgacttcac tcagcatcca gtagatgggt ttgcgttcaa attttgctga
    18601 ggttgcgggt tgggcgagga tcacatagcc catattccct gcagattcat gagagataag
    18661 gtcttcttgc tctgttggca tcaggtcggg caacaggata tcgcaatact tagccgaggc
    18721 cgtcatgaag aggtcaatgc caacaatcat ctcgcacttg ctgtcatcct gaagcacctc
    18781 atgggtgtga ttgatgtcgc catgttgatt gatcaatgtg ttactggcgt agcaccataa
    18841 aaacttgatg gggacatcca gtttttcttt tccacgaaca ccatcacggg tcgcggtcat
    18901 ttccgtacca tggtcgatgg catctgtcca tgtaaagacg gaaatctgcg ttttaacagg
    18961 attctcaagc atcgggaacc attctacccc cagatcccag ctaccttcgc gtacacctga
    19021 gttgccgccg tttatgccga cgttaccggt gagaacggaa agcatggcaa tagcgcggga
    19081 cgtttgctcg ccgttggaat gtcgttgtgg cccccaaccc tgacaaatat aagcaggttt
    19141 tgctgaaccg atctctcgtg ccaactggat aattttttct gccgggatgc tggtgatttt
    19201 tgctgcccat tccggcgttt tagctatgcc gtcaggccct tcgcccagaa tataggcttt
    19261 ataatgcgcg ttacgtggtg cgttggcggg cagcgttttt tcatcgtaac caacacaata
    19321 tttgtcgaga aatggctgat cgaccatgtt ttcagtaatc agtacccagg caatcgcaca
    19381 ggccagtgcg ccatcggtgc cagggcgaat gggcagccat tcatcttcac gcccggcagc
    19441 agtgtcgtta taacgtggat cgatgacgat catgcgtgcg tttgaacgtt cgcgggcttg
    19501 ctcgacgtag taagtgacac caccgccgct catccgcgtt tctgccgggt tatttccgaa
    19561 cataacgacc agtttcgtat tggcgatatc atccgggctg ttgccatcat tggcaccgaa
    19621 catataactc attgcggcac tgatctgtgc ggtactgtag ctgccatagc gactgagaaa
    19681 accaccgcaa gagttcatca gacggtacgg gacgtttgag ttggtgatgt ttccgccatc
    19741 tacgcctgtt ccgtacagga catgtacagc ctcattgccg taatctttca ggatccgccg
    19801 aagattatca ctgatggtat ccagggcttc gtcccaactt atccgttcaa atttaccttc
    19861 accgcgcttg ccgacgcgct tcatgggata tttcaaccta tcaggatgat tcatccgtcg
    19921 gcggatagag cgcccgcgta aacacgctcg aacctgatgg ttaccgtaga cgtcgtcacc
    19981 tgtcgtatca gactccaccc agtacacggt gtcatctttc acatgcaaac gtaacagaca
    20041 gcggctcccg cagttaacgg tgcaggaact ccagaccgct ttctcttcta ccggagcctc
    20101 tgccgcccgg accatttggg aaaatggcag agtgaaagca ctgcttgcca gcgcaagact
    20161 gccaagtgcg gaggttttca tcagacttct acggctgatt tcagccttca tgagcgcctc
    20221 tgtggtatgg attttcatca ttactcactt attgcttttc aaacaaaatg tcatgccaga
    20281 atttatgatt gtcgtgggtt atattttttc gatctcgacc agattagtgt gctgcgggtt
    20341 tcccttcgcc agtggtgaag ggcgcagagt ggttagcgta ttcacacagc cgccatggtc
    20401 gattttatcg ccagacatat tggcctcgtg ccaggctccc tggcccatag cgctaactcc
    20461 agggagaata cgtggtgtta ctttggctgg tagccgaact tcgccacgat ggttaaacac
    20521 ccgcaccata tcgccgttgg caatcccacg tttctgcgca tctatagggt tgatccacac
    20581 ctcctgacgg caggcagcct tcaggagatc aatattgccg taggtcgagt gagtacggga
    20641 tttgtaatgg aaaccaaaca gttgcagtgg gaaggttcta cgttcagggg agtcccagcc
    20701 ttcaaaggta gaggcataaa ctggcagtgg gcttatcact tcatcttttt ccagttccca
    20761 ggtacgggca atttccgcca gcttgctgga ataaatttca atcttaccgg aaggtgtttt
    20821 aagtggattt gcctcggggt cgtcacgaaa tgctttgtag gcgacaaaat gaccattggg
    20881 atctttacgc ttatagatac ccattttttt cagttcgtcg taagacggta acgccggatc
    20941 tttggcaagc attttggcgt acagatgttg taaccattgt tcctgcgtgc gaccttcagt
    21001 gaacttttga tagacgtcag gtccaagacg tttcgcgact tcactcagga tccagtaaat
    21061 cggtttgcgt tcgaattttt cgctggtgac aggctggagg aaaatgagat atcccatgtt
    21121 accggcgtag tcgttaggaa taatatcttc ctgctcaacg gtcatcaggt ctggcagcag
    21181 aatgtcggca tattttgccg atgaggtcat aaagttttcg atgaccacaa tcatttcgca
    21241 tttcgattcg tcctgcagaa tttcatgcgt tttgttgatg tcagaatgct gattaacgag
    21301 ggtatttccc gcgtagttcc agatggactc cattgtcaga cggatggatg gacttgaagt
    21361 agttgactca atgctactta aggtcctgat atgactgaca ttaaccatga taatactgta
    21421 tatccatcca gttctttctc tgggtctccc tatctgtatc agcaagtctg cacctcaagt
    21481 cctgacacaa ggctgacaca aatcaatcta ctcagcgtag ctaagcctca gttagtccgt
    21541 agagccaatg gtcgatatac tatccgcttc agactcaagg gacagaccat cccgtttctt
    21601 tcagtatcga cccgaagcac agacaggaga gtagccacaa tgcgtcagag agagcttgca
    21661 gccacagcta agtccttcat gttggatagg cctgatgtct cgctacagga gcttacggac
    21721 cacctccggt ctatggctga gcagttcctg acagacgcca gtgatgacta ctggaatgga
    21781 cttgaggtcg ctactctggt ggacgagaag tccaacctga aggagctagc cgctacgcaa
    21841 gccttgagtc tggaccagca gaagggcata aggttagcgc ttgaagtcct gaccgcagca
    21901 cagcagaggg tagacactgg tgatgcctct ggtctgatta agctgattga tgataataac
    21961 catactgatt actcaactat aggcgactct acgtcaattc tgaagaatga gcagggggat
    22021 agaccagcag ttttcacaca ggagcgtcag ccatctgtgg ttttttcaag cctagtgtcc
    22081 tcgctgctgg ctgagaaggt ccagaccctg aagtcctcca gctacaagga cctgtcgtcc
    22141 tcactgaata ccgtctctcg gttcttacct gaagacatgg acctgatgtc ccgctctgag
    22201 tggctggctg tcagggactc catgctggct gctgaggtga gaccatcgac aatcaacaag
    22261 ctgctgacta aggcgaagat gtgtcttgat tatggcctga tgaatgggca acttgagggc
    22321 cgcaacccta ttgaacggat gaagctgacc aaggatatcg actccaagcg cagagcgttc
    22381 acagacgagg agctggagag acttctggtc cgtgttgagt ctgagtatca gttcacacga
    22441 cacacggctc acacgacctc tgaggcccgt agatgggcgt ctctggtgtc tgtggtcact
    22501 ggcgcgaggt ctgctgaagt ctgccacctg actaaacgtg acattgtcac cttagacaac
    22561 ggtttaacct gcatcgacat caatgaagat ggggacggta agtctgtgaa gaacaaacat
    22621 tcagtccgac ttgtaccgct gaccgatggg gcctgtgggt tcgacctgaa gtccttcctt
    22681 gaatgggtag acacgcagcc tgacgacggc ggtcctctct tcgggatgac tccgagcgcc
    22741 tactctagct ggtttaactc tagggtcctg actgaggcac tgggagacgc tgagaacgtt
    22801 tctctccata gtctgaggca ttggctggcg acccgaatga aagagcgtgg agtcaatctg
    22861 gtggacgctc agggcatctt aggccatagc agtcagtcaa ttacgtatga cctgtacggg
    22921 aaaggacacg ctgttggtag gctggctgag gtgctgaaga cagctttact ttaagtagga
    22981 agacccaagg tggagaccat atacggcttg atggttgaga ccttgggtca ctgaagtctg
    23041 actgaagtta gggagggtga taataatcat aatccctcct gactataggc ccactggttc
    23101 gccttcggct gtcagactct aagtctgtat gtggtcttat ttaaactggt tccctaactc
    23161 cagtactcca aattcctatg gtctcctctt gacattcctg tttaactgtg agtcgcctct
    23221 ggtctctctt tatatatact tccttaattc ctgaggcgtc tttagtcctt gacatccagt
    23281 ccagcctgtg agtcacatcc ggtcctccct ctttatatat acttccttaa ttcctagggt
    23341 cgccttaatc cttgacatcc agtccagcct gtgagtcaca tcctgtcgcg ctactgtatg
    23401 catcaccagt gatggacttg attatcacta taggggaacc aactaattca actacaggag
    23461 atactctatg gcacgaccaa tcgcaactca cgacaacacg ttcaccaaag cctacttgca
    23521 gcagcactgc ggggacctgc tgtcattcga tggtcagggc gacctgagtg gttggctgga
    23581 tgatgtcctt actggtgctg gtcgcctgaa cgaatcaatg gcaagcaata ccaagcctgt
    23641 cagcccatac ttgattctga cccaactact aacgcacgac acattaactg taagcgctgt
    23701 acaggagtcc ctgagccgta agcgtgttgc cttgggtgaa cctatggttt caactcgcta
    23761 cgctcgctac gtctatgcta ccgtcgtcag cgccagtaaa tccgtccagt accacgccag
    23821 taaagctggt tcatgattac ggaccctaaa ctatcactat agggagacca gcctccgcct
    23881 ctgagtctga tgcgtcagaa catgcgagca tctggtcaac cgacattaag tatctctcct
    23941 aagtaatcaa ggcatgtgac cgcctctgtc agacctcgtg tatcgacctc cagctcgtta
    24001 gcgttctcgt gtcttcatta tgatttgggt ctatcaccgc tcaccatctg gaagtcctta
    24061 cagctcgtcc tgacatgcgg tctcaccttt atatggctgg aagagacctc ctctgtcaga
    24121 cgctatctaa ccaacccaga aaacgggttt gttaaaaaga ttaacatggg tctccacctt
    24181 catgaggacc gctccggaaa tttccggacc gtgaacgggt ggctctatca caggtcgcga
    24241 aaatggaaac ctgcttaacg tccccaaaac tgagcagtca ccattcttaa cgggtccaaa
    24301 tttgggcgcg ttggcgagaa gtctaaccaa cccagaaaac gggtttgtta aaaaagttaa
    24361 catggtcttc acacgacctc attacctcaa gtctttctgt atgtctcctg ttgcctgtga
    24421 gtctccctat cgttgggggc ttgagtaggc tttcagggtg tgacttcagg aggcataaca
    24481 aaaggagtac aacgatatgt caactcaacc cttaatgaag catgaagtag ttcttcctgt
    24541 catcgttgac ggctatcact gggcctgtaa tcaggaccgt atgtggaacc tgaacgagct
    24601 acataaagag cttgagcttc catctgctaa ggagccgggt cagtggcgtt ctagcgtcag
    24661 gacgcttctt gaccaagatg caaatctgca tgttgctcat ggtggttcga ctccggggac
    24721 atgggctact gaagaggcgg ctattgccta tgcgatgtgg gtctcacctg atttctacct
    24781 taaggtcatc cgtgctttca tcgctttacg taacgacaaa gtgtcggaag ctaaggcgct
    24841 ggcgcaggac tccaaggaac ttaagggtct caagcctata gcccgtaact ggctggaaaa
    24901 gctggataac ccgaagcagg gccacaccct gacagagtgt cttaagaatc tcgacttcag
    24961 gttaggtaca aagctggtat cagccaaggc tcttaatgcc gtattgaagt cagggcgtat
    25021 agctaatccc ttctatagtc gcaagctgga cggtcgagga cagcctgtat ttgacatctc
    25081 gtcaggtggc tggatgacca gcttcaatcc taagggtctg gagaacggct attaccgtct
    25141 tcaggataac cactataacg gtcaggaggg gcttaaggtc ctcaccaagg gttacgagtg
    25201 gcttaagtcc aacaaggtcg agctggttcg tctgtgcgct aagggaggac tctaagatga
    25261 ttgacccgcg cacacctgag ggccgcatga ctctccgtta ccgtggctac cggactgaag
    25321 tcctcctcag agagcttggt ctagacccgg aagatgagac ccgccagcac caatcccgag
    25381 acgagctgat agcgcaactg gtcgctatga aactgtctgc ctaattttaa aaccttttga
    25441 gatgaccgat gcggggagac ccgtaggcgc tactgcgtct gcgataccga tagtctcaac
    25501 tctcaagagg agaattacct atgatgttca acaacaacaa ctggaagctg tcagtcaccg
    25561 acatcaacct ttatgaaaac actgtgagtc tggacggtca gtcctatccg ttgtcgctcg
    25621 ctatcaagac cctgattccg ggctacctgt caggactgcc gtctaccagc cgtgagtcta
    25681 tggagttact ggaagctctg gctgaagctg gtgtagctat tggcaacttc ttcagtaatg
    25741 acctgatgac cgcctatggt cgccgccagc agaacaagcg agctgaggct gagcgtatcg
    25801 ctaaagagca gcgcattcag gccgagcgca tgcgtgaaga gaacatgact gatgcagagt
    25861 ggcagaaaga gctacagcgc cgcgaacagg tcaaagctga acgccggacc tatggtgaga
    25921 acctccgcag tgctacccat tccgctggtc gctcccgtgc cgctattgtc gctgaccttg
    25981 agtcaggcgg taactggctg gacagcttat aagacacaca gtccctgacc cgatgttagg
    26041 gactcctcag ttttgaggac tcacagtatt taaactaatt taggagattg attattatga
    26101 cgattaaaga cgctttccag tttggcattg agccagtacg catcaccgat accgacaaca
    26161 ttcaggtcaa tgaaggtctg ccgactaacg ctgacccgca ggtctacgcc ctccagttag
    26221 ctaagacggt taaggctatg ctgaacggtg tacttaagga cgctcaagag aacatcccgt
    26281 tccctgtaga agtcctgccg acccgtaaca gcttaccgac cccaatcatt gcccacactc
    26341 tgactgaccg ctctgtggtc gttcctgtcc gtggtggcaa gcgacctgaa gtcgtgaccg
    26401 ttccgtctgg acaagaaatc gtggtcgaac caatcgagca ggctattctc atctctgagc
    26461 agaccaagct ctgggacgcc aagtcatcca ccggattcac tcagggcaca cttcagcagg
    26521 acgctatgaa catctgtgag aatgtggtcc gtaccatcaa cgctcgcatg gtcgacgttc
    26581 tggaatcctc taagctgctg aagaccgtag agcttcctgt cctgactggt agcctgaccg
    26641 ctaaggcaga cgctatcatg gacgctctgt atgagaacac agagtcttct ttcggttctg
    26701 aggtgtctga ctacggtatt atcgctcatg agagccaact gaaagcactg tcccgtctgg
    26761 ctgctaagca aggcttctcc ggtgaagacg ctatcgtcga tatgctggga acagacatcg
    26821 cctactacaa cggtgaagac aagggtgtat tcatgctggc taaacgcttc acggctctga
    26881 gcttcggttg cttccgtcac gatggcgaga atattacggt cgtcctttca cgtgatggcg
    26941 actctcagtc ccacgacttg gaaatcctcg gtaaagtgtt cgttgtcgct gaagctgcta
    27001 cgaccatcaa gatgggcact ggctcagcta ctgccgtcct gcctgtagtc aaacgcctga
    27061 agttcaccaa gaccgaagcg taagaccaat gggtaagagc attagtaaag gcttcaggtc
    27121 catcgccaag ggcctgacag gtggtggtgg gactcaaggt ggggcttcgg tcccgcctcc
    27181 tgtagctcct cctgtagctc ctccagatga cgctcagcaa gctgtagtcg atacccaagg
    27241 tcaggagacc gagggaggta agaccatgag cagaggtaag tctggactga atatcagcag
    27301 gacttcaggt ggtggcctgt cactgtgatt aagacatcta tacagcctgt ggagggtgga
    27361 cgatatcaag caacaatctg gatgacacgc agagtctata agcggctatg ctggtcactg
    27421 gctgaagctg aacagtgggt gagacgtaaa gtgcctaccg ttcaatcgtt ccaatcatca
    27481 tgataactat ggtctggacc aatggtctgg acctttgttt tgtactagtt atctagtgca
    27541 gtagtacaat agacgaagga ggctttatgt ctatgacttc cgcagaccta aggtccctcc
    27601 tgactctggt ctataaactc gtgttcctct ctgtgggtct ctatatggtc ctctccggtc
    27661 gactcggtgt gaatgtattc gacactctgt ctaaggctgt gggtgggctt ctgggggctt
    27721 aggtttggac tcacagtggg accgatggtt tctgacagaa aaatgtgaat gaccatctaa
    27781 tacattaagc cagcagactt cccccatagc ccgcctcaag tccagaccga ggccaccggg
    27841 gggctgacca gccgtctaga catcccgtct aatgacacaa ggctgacaca aagcctgaca
    27901 caaagtcctt gactcaatgc aggacctcag gcctactaag ggtccgcttc aagtccttga
    27961 agttccagat gaacttaatg ggcacatcca gtttatcttt gccgcggacg ccgtcgcgga
    28021 ttgccgtcat ttgcggacca tgatcgatag catctgtcca gctgaagcag gagattgaca
    28081 ttttgaccgg attatccagc accggcaggc gttctatggt aatggtatag gtcgattcac
    28141 gcgcgccact atttccgccg ctgatgccga cattgcccgt caaaataggt aacatagcaa
    28201 tagcgcgtgc agtcagttcg ccgtttgcct ggcgttgcgg cccccagccc tggcagatat
    28261 aagcgggttt tgctgtgcca atttcacgcg ccagtttgat gatacggtct accgggatac
    28321 cggtaatttg cgaagcccac tgcggcgttt tcgctgtgtt atcatcacct tcaccaagaa
    28381 tataggcttt atagtgacta tttttgggtg catctgcggg taaggttttt tcgtcatagc
    28441 cgacgcagta tttatcgaga aaaggttgat caacgagatt ttcgttaatc aatacccagg
    28501 caatacccgc aaccagcgcg gcatcggtgc ccgggcgaat agggagccat tcatcttcac
    28561 gaccagcagc cgtatcggta tatcgcggat cgatagcaat cattttggcg ttcgatttct
    28621 cgcgcgcttt ttcaagaaga taagtgatgc caccgccgct catgcgggtt tctgccgggt
    28681 tgttaccaaa catcacgacc agcttgctgt tttcaatatc cgtggtgctg ttgccatcat
    28741 tactgccgta ggtgtagggc atggcacagg aaatttgcgc ggtgctgtag gagccatact
    28801 gattgagtga accgccgtag cagttcatca ggcgtttgac cgccgaggct gatggcgaag
    28861 agcgggtcat attgccgcca acgatccccg aagagtactg aatatataca gcctcattgc
    28921 catattgttc gacggttttc ttcaggctac tggcgatagt atccagggct tcatcccagc
    28981 taatccgttc gaatttgcct tcgccgcgtt tgcccacgcg tttcattggg taattcaagc
    29041 gatcgggatg attaatacgc cgacggatgg atcgaccacg caaacaggcg cgtacttgat
    29101 gattgccata ctcgtcgctg ccggtgttgt cagtttccac ccaggtgact tcattatctt
    29161 taacatgtag acgaagtgca cagcggctac cacagttgac ggaacaggca ccccagatca
    29221 ctttttcgct ggcctgttgt accgctgccg ctgcactgcg cagggtgaac ggtaaagaaa
    29281 aaccgcctgc agccagcgcc agagaaccta tcgcggttga tttaacgagt gttctgcggc
    29341 tgatgcccac cattcggtca tttttggaca taactcactc cctgttcttt atcgttatat
    29401 aaatgtttat atattgaata tttagcgcgc taacaataga gggagtctac ccattttggg
    29461 ttaagaatta ttaatccata tcaatagaag ggtatgagta ataaggtggg attatgttgt
    29521 atgttcaaat cgccggatgt gtcgtatccg gcgttcagtc gataatgtat tactgcggtt
    29581 cggcaggcgc gccatcctgg gtagactgcg cgggagcaga gacgttaccg ctggtggtgc
    29641 gggtatagag aattttatgc gtatcattag cgcaatggcc gacgacctgg gaatcaggct
    29701 gatcaacctg gtcattgggt acaatactta acgtgaagct gctttcgggt acgccattat
    29761 tgataatgcg ctgtgatata tcgctctgta tgcgctcaca ggatcccggc gcggcgagta
    29821 ccgcgggtga ggcgagggcg agcagaagcg cggcacagca ggttgagagt ttcatcataa
    29881 gctccttacg cgaagataac ttctttaagc atagcattta acgtgtaaag tactgtattt
    29941 gctactatga ttgagaatca tctctactct ctggtgactg ttgtgaaata caaattacta
    30001 ccatgcttac tcgcgatatt cctcacagga tgtgaccgca cagaggtaac actttcattt
    30061 acccctgaga tggccagttt ctctaatgaa ttcgattttg atccgctgcg tggtccggta
    30121 aaagatttca ctcagacatt aatggatgag caaggtgaag tgacgaaacg tgtttctggg
    30181 actttgtcgg aagaaggctg ttttgattca ctcgaattac tggatctgga aaataatacc
    30241 gtggtcgctc tggtactgga cgccaattat taccgtgatg ccgagacgct ggagaagaga
    30301 gtacgtttac agggaaaatg ccagctagca gaattacctt ctgccggggt gagttgggaa
    30361 accgatgata atggcttcgt gattaaagcc agcagcaaac aaatgcagat ggaatatcgc
    30421 tatgatgatc agggttatcc actgggtaaa accacgaaaa gtaacgacaa aacattatct
    30481 gtcagcgcca cgccatcaac ggacccgatc aaaaaattag attacacagc ggttacttta
    30541 ctgaataatc aacgagttgg taatgtaaaa cagagctgtg aatatgacag tcacgccaat
    30601 ccggtggact gtcagctgat cattgttgat gaaggagtaa aacccgccgt cgaaagggtt
    30661 tacaccatca aaaatacgat cgattattat taatgctatt gtgcggtcgg cttcaggaga
    30721 gtctgacccg gtgttttgtg ctctgccaga tactgatgct ggaatataca catgcgaatg
    30781 gcattacgat attgaccatt aataaagaac tcgtgcatca attcaccttc aaccgtaaag
    30841 ccaagcttgc ggtaaatgtg aatcgctttt tcattctctt tatcgacgat cagatacagc
    30901 ttatagagat tgagaacggt gaagccatag tccattgcta atttggcggc acgggttgcc
    30961 agacctttcc cctgatactc cggggagata attatctgaa attctgcccg gcgatgtaca
    31021 tggttaattt ccaccagctc caccagaccg gctttttcgc cgtcacattc caccacaaag
    31081 cgtcgttcgc tctgatcgtg aatatgctta tcatacagat cagagagttc aacaaaggct
    31141 tcgtagggtt cctcaaacca gtaacgcatc acactggcgt tattgtcgag ttgatgtaca
    31201 tagcgtaaat cttcacgctc cagcgggcgt agcttaacac tgtgggcgct tggcataacg
    31261 tgtccttaca ttccttaaat caataacagg ttagggggta ataacgcggc cagttcgacg
    31321 gtccaggcag cgcaaagtat ttggctccca gtaggcattg atgttggcgc tttgctcaca
    31381 tttatcgcgg ttatcaaaag cggcgtcggc tttatcccac tctttttcag tgcgtttatt
    31441 cactttctga cgcagattgc gcgtgtcatt ccattgctct ttttccatag cggcgtgctg
    31501 gcggctttgt gcactgtcgc cagactcaat caccagtttg ttagtttcgg catgaacagt
    31561 tgtgctcagt gccagtgcgc aaggcagcag aaaagcgagc aggccgattc gtttgctgag
    31621 agtgattttc ataattcatt ccctgtatga atgattaaag gagattctac accatccact
    31681 gcgaacgcaa aacgtaccag gagggtgttt atattgatga tattatgtcg ccctataact
    31741 atacatgatg tcaataagtg acaaagatga ttaaagcaac gttactattt tttgctactg
    31801 cgctgtgtga aattattgga tgctttctgc cctggttgtg gttaaaacga aacgccagta
    31861 tctggctgtt gcttccggcg gggatttcac tggcgctgtt tgtctggttg ttaacgttgc
    31921 atccagcggc gagtgggcgt gtttacgcgg cttatggtgg cgtttatgtc tgcacggcgt
    31981 tgatgtggct gcgcgttgtg gatggcgtga aactgactct ttatgactgg acgggtgcgt
    32041 tgattgcgct ttgcggcatg ttgatcattg ttgcgggctg ggggcgcacg taggaacata
    32101 aatccatttt atcaataaga taagaggaag tgtcagctga caaaaggtat tctatttcat
    32161 cttttgtcaa ccattcacag cgctaatata cgcctttttt tgtgatcact ccggcttttt
    32221 tcgatcttta tacttgtatg gtagtagctc agttgcgtag atttcatgca tcacgacaag
    32281 cgatgcaagg aatcgaacat gaagatcgta aaggctgaag tttttgttac ctgtccgggg
    32341 cgtaatttcg tcacattaaa aatcaccact gaggacggta ttacgggcct tggggatgcc
    32401 accctcaatg gacgtgagct ttccgtggcc tcttatttgc aggatcacct ttgtccgcag
    32461 cttattggtc gcgatgcgca ccgtatcgaa gatatctggc agtttttcta taaaggtgct
    32521 tactggcgtc gcggtccggt tacgatgtcg gccatttcag cggttgatat ggcgctgtgg
    32581 gatattaaag ccaaagctgc caacatgccg ctttaccagt tactcggcgg cgcgtctcgt
    32641 gaaggggtga tggtttattg ccataccacc ggtcacagta ttgatgaagc tctggatgat
    32701 tatgcccgtc atcaggagct gggattcaaa gccatccgcg tgcagtgcgg aatccctggt
    32761 atgaaaacca cctacggcat gtcgaaaggt aaaggtctgg cttatgaacc cgcaaccaaa
    32821 ggacagtggc cggaagagca gttgtggtcg acggagaaat acctcgattt catgccgaaa
    32881 ttgtttgacg cggtacgtaa caagtttggt tttaatgaac atttgctgca tgacatgcac
    32941 catcgcttaa cgcctattga agcggcgcgc tttggtaaaa gcattgaaga ttatcgcatg
    33001 ttctggatgg aagacccgac gcttgcagaa aaccaggaat gcttccgtct cattcgccaa
    33061 cataccgtca cacccatcgc agtgggtgaa gtcttcaaca gcatctggga ctgcaaacaa
    33121 ctgattgaag agcaactcat cgattatatc cgcaccacgc tgacccatgc aggcggaatt
    33181 accggtatgc gccggattgc cgattttgct tcgctgtatc aggtacgtac tggctcacac
    33241 ggtccttccg atttgtcacc agtctgcatg gctgcagcgc tgcactttga tctgtgggtc
    33301 cccaatttcg gtgtccagga atacatgggt tattccgaac aaatgctcga agtcttcccg
    33361 cacaactgga ctttcgataa cggctatatg catccgggag acaaaccggg tcttggcatc
    33421 gaattcgatg aaaagctggc ggcgaaatat ccctatgaac ctgcttatct accagtcgca
    33481 cgtctggaag atggcacgct gtggaactgg taaggagtaa gataatgaaa agcatattaa
    33541 ttgaaaaacc gaatcaactg gcgattgtcg aacgtgaaat acccaccccg tcagcgggtg
    33601 aagtacgagt aaaagtgaaa cttgccggaa tttgtggttc agatagccat atttatcgtg
    33661 ggcataatcc ttttgcgaaa tatccgcgcg tcattggaca tgaattcttt ggcgtcattg
    33721 atgcggtggg tgacggcgtg gaaagcgcca gagtcggtga acgtgttgct gtcgatccgg
    33781 tggtcagctg tgggcattgc tatccgtgct ctataggtaa accgaacgtt tgtacgacac
    33841 ttgctgtatt aggtgtgcac gctgacggtg gtttcagtga atatgccgtg gttccggcaa
    33901 aaaatgcgtg gaaaattcct gaagcagtgg ccgatcaata tgcggtgatg atcgaacctt
    33961 ttaccattgc ggctaacgta accggacatg gtcaaccgac tgaaaatgat accgttctgg
    34021 tttatggtgc cggtccaatc ggcctgacga tcgttcaggt attaaaaggc gtctataacg
    34081 ttaaaaatgt gattgttgcc gatcgcattg atgaacgact ggaaaaagcg aaagagagcg
    34141 gggcagacag ggcgattaat aacagccaga caccgcttgg cgagattttc gctgaaaaag
    34201 gcatcaagcc gacattaatt atcgatgcgg cttgtcatcc ttctatcctg aaagaggccg
    34261 ttacgctggc ttctccagcg gcacgtattg tattgatggg cttctccagt gaaccgtctg
    34321 aagtgattca gcaaggaatt accggaaaag aactctctat tttctcttca cgcttaaatg
    34381 caaataaatt tccggttgtt atcgactggt taagtaaagg gttaattaaa ccagaaaaat
    34441 taattaccca tacgtttgat ttccagcatg ttgctgatgc cattagttta tttgaacagg
    34501 atcaaaaaca ttgctgcaaa gtcttactca ctttttctga ataataccaa taacggcgag
    34561 taagtagtac gcatcttacc tcttttttag agataaccat tatgacaata gaaaaacatg
    34621 aaagaagcac taaggatttg gtgaaagcag cagtatcggg atggctgggc actgcgcttg
    34681 aatttatgga tttcaagagt catgcgtgtt aactatttga taaatattaa attaattttt
    34741 cattgattcg ttatggggca tggttggggc aaactcgctt aactgtgtat ttaacaaagc
    34801 tacctgtgca ttattgtttt cagacatcca ttttccgtat acctgaaata ccatttgcgc
    34861 atctgcatgg cccatctggt ttgctataaa tgccgggtta gcaccagctg tcagcgacca
    34921 gcaggcataa gtatgtctcg actgatatga ttttcgatgg cggagtccgg cacgttttat
    34981 cgctgcgtcc cacatctgcc ttattgagtc aacggtaaaa tggtcaccat aattttttac
    35041 tctcgctgac acttcaggtt gaaaaacaaa ggtgcatttt tgtttttctg ttctgccata
    35101 ctctctgagg tgaacatcaa tgatatgctc tttgctcagt ctcgttaatg tcatctgact
    35161 ccggagagcg tcgattgctg gcttaataag atgaatggcc cgattggttc ccgcctgtgt
    35221 ttttggtacc gtgaaacggt cttttgctaa atttctcctg atcatcattg ttccattttt
    35281 cagatctatg tcctcccatc caagtgcaca cagctcacca gggcgaacgc cagtataaac
    35341 agaaacacac cataaatttt ttgcttgctg atttctgcac gcatcgataa gacggataaa
    35401 ttcctcccgc gaaagaggat ccggaatggt tcttgattcc tttaatggcg agatcccctt
    35461 aaacggatta tctgccaggt aaccgttatc aacaccaaac tggaacacgg cgttaagatt
    35521 tgtcatgtaa ttatttacag ttacagccga tctccctggt tgtgtaacaa tatagttact
    35581 tttggggatc tggtatccag tcagtagctc tttacgaacc tccagtaatt tttctttatt
    35641 aatcgatgag gcaagatttt tttcaccgat tatgctcagg atatttttga tgacggcacg
    35701 gtatgtgttg agtgatgttt tggcgacttc agtttctttc agtgccagaa atttttcagc
    35761 cagttctttt atggttaaat cttgtcgggc ctcaccaaat ttttccagat tgcgtgagga
    35821 gggaaactgt tttgcatagt cgaaaacacc agtttttatt gcgtaacaaa cagaggagcg
    35881 tagttcacct gcaacgcgcc tgttttttgc tgtgtcagga acccccagat tttccctgac
    35941 tcttacgcct ttataaacaa accagatacg taatttccct ccatggtttt ccacgcctgt
    36001 cggatatttc atttcaactt ctctcattag ttagtgtggc ttttagtcaa gtaagatgac
    36061 atcttggtct cgctgatgct tggcgctcaa tccagcgatc aatttcttcc aggttgtaaa
    36121 agcatggact gttatcccat ggcataccgt catgagcgac atgcttatat tcccttcctt
    36181 ccataaacga tttttcccga gcctttttta acgtaccttt ttttattcct ttcagcgcaa
    36241 ttaactgctc ttcggatacc catttgccgg gagagacaat catgattact tcgctcatcg
    36301 atttctttat ctcttacatc agacgagcgc cggttgcaga ataccagtca caaccggcga
    36361 cagttgaaca ttaagaatca gcctgactcg ggatcagttt ttgccagata actgaaacgt
    36421 attttgcctg gtaacgggcg tcatcaagtg cattatggcg ctcaccttcg aatggaatag
    36481 c
//
LOCUS       DAZFQD010000035        36225 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.035, whole genome shotgun sequence.
ACCESSION   DAZFQD010000035 DAZFQD010000000
VERSION     DAZFQD010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 36225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36225
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.035"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1092
                     /locus_tag="ACHKTW_001830"
     CDS             <1..1092
                     /locus_tag="ACHKTW_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1-like element ISEc1 family transposase"
                     /protein_id="HFO6462863.1"
                     /translation="YRQAWKVEHKLSDILLLTICAVISGAESWEDIEDFGETHLDFLK
                     QYGDFENGIPVHDTIARVVSCISPAKFHECFINWMRDCHSSNDKDVIAIDGKTLRHSY
                     DKSRRRGAIHVISAFSTMHSLVIGQIKTDEKSNEITAIPELLNMLDIKGKIITTDAMG
                     CQKDIVEKIQKQGGDYLFAVKGNQGRLNKAFEEEFPLKELNNPEHDSYAISEKSHGRE
                     EIRLHIVCDVPDELIDFTFEWKGLKKLCVAVSFRSIIAEQKKEPEMTVRYYISSADLT
                     AEKFATAIRNHWHVENKLHWRLDVVMNEDDCKIRRGNAAELFSGIRHIAINILTNDKV
                     FKAGLRRKMRKAAMDRNYLASVLAGSGLS"
     gene            1361..3598
                     /gene="nrfB"
                     /locus_tag="ACHKTW_001831"
     CDS             1361..3598
                     /gene="nrfB"
                     /locus_tag="ACHKTW_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-3',5'-guanylate-activated
                     glycosyltransferase NrfB"
                     /protein_id="HFO6462864.1"
                     /translation="MDWLLDVFATWLYGLKVIAITLAVIMFISGLDDFFIDVVYWVRR
                     IKRKLSVYRRYPRMSYRELYKPDEKPLAIMVPAWNETGVIGNMAELAATTLDYENYHI
                     FVGTYPNDPDTQRDVDEVCARFPNVHKVVCARPGPTSKADCLNNVLDAITQFERSANF
                     AFAGFILHDAEDVISPMELRLFNYLVERKDLIQIPVYPFEREWTHFTSMTYIDEFSEL
                     HGKDVPVREALAGQVPSAGVGTCFSRRAVTALLADGDGIAFDVQSLTEDYDIGFRLKE
                     KGMTEIFVRFPVVDEAKEREQRKFLQHARTSNMICVREYFPDTFSTAVRQKSRWIIGI
                     VFQGFKTHKWTSSLTLNYFLWRDRKGAISNFVSFLAMLVMLQLLLLLAYESLWPDAWH
                     FLSIFSGSAWLMTLLWLNFGLMVNRIVQRVIFVTGYYGLTQGLLSVLRLFWGNLINFM
                     ANWRALKQVLQHGDPRRVAWDKTTHDFPSVTGDTRSLRPLGQILLENQVITEEQLDTA
                     LRNRVEGLRLGGSMLMQGLISAEQLAQALAEQNGVAWESIDAWQIPSSLIAEMPASVA
                     LHYAVLPLRLENDELIVGSEDGIDPVSLAALTRKVGRKVRYVIVLRGQIVTGLRHWYA
                     RRRGHDPRAMLYNAVQHQWLTEQQTGEIWRQYVPHQFLFAEILTTLGHINRSAINVLL
                     LRHERSSLPLGKFLVTEGVISQETLDRVLTIQRELQVSMQSLLLKAGLNTEQVAQLES
                     ENEGE"
     gene            3585..6557
                     /gene="nfrA"
                     /locus_tag="ACHKTW_001832"
     CDS             3585..6557
                     /gene="nfrA"
                     /locus_tag="ACHKTW_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
                     /protein_id="HFO6462865.1"
                     /translation="MKENNLNRVIGWSGLLLTSLLSTSALADNIGTSAEELGLSDYRH
                     FVIYPRLDKALKAQKNNDEATAIREFEYIHQQVPDNIPLTLYLAEAYRHFGHDDRARL
                     LLEDQLKRHPGDARLERSLAAIPVEVKSVTTVEELLAQQKACDAAPTLRCRSEVGQNA
                     LRLAQLPVARAQLNDATFAASPEGKTLRTDLLQRAIYLKQWSQADTLYNEARQQNTLS
                     AAERRQWFDVLLAGQLDDRILALQSQGIFTDPQSYITYATALAYRGEKARLQHYLNEN
                     KPLFTTDAQEKSWLYLLSKYSANPVQALANYTVQFADNRQYVVGATLPVLLKEGQYDA
                     AQKLLATLPANEMLEERYAVSVATRNKAESLRLARLLYQQEPANLTRLDQLTWQLMQN
                     EQSREAADLLLQRYPFQGDARVSQTLMARLASLLESHPYLATPAKVAILSKPLPLAEQ
                     RQWQSQLPGIADNCPAIVRLLGDMSPSYDASAWNRLAKCYRDTLPGVALYAWLQAEQR
                     QPNAWQHRAVAYQAYQVEDYATALAAWQKISLHDMSNEDLLAAANTAQAAGNGAARDR
                     WLQQAEQRGLGNNALYWWLHAQRYIPGQPELALSDLTRSINIAPSANAYVARATIYRQ
                     RHNVPAAVSDLRAALELEPNNSNIQAALGYALWDSGDIAQSREMLEQAHKGLPDDPAL
                     IRQLAYVNQRLDDMPATQHYARLVIDDIDNQALITPLTPEQNQQRFNFRRLHEEVGRR
                     WTFSFDSSIGLRSGAMSTANNNVGGAAPGKSYRSYGQLEAEYRIGRNMLLEGDLLSVY
                     SRVFADTGENGVMMPVKNPMSGTGLRWKPLRDQIFFLAVEQQLPLNGQNGASDTMLRA
                     SASFFNGGKYSDEWHPNGSGWFAQNLYLDAAQYIRQDIQAWTADYRVSWHQKVANGQT
                     IEPYAHVQDNGYRDKGTQGAQLGGVGVRWNIWTGETHYDAWPHKVSLGVEYQHTFKAI
                     NQRNGERNNAFLTIGVHW"
     gene            6558..7448
                     /gene="ybcH"
                     /locus_tag="ACHKTW_001833"
     CDS             6558..7448
                     /gene="ybcH"
                     /locus_tag="ACHKTW_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HFO6462866.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRLQGFDTLVLQWTRYGDAFTQPEQRALLFKRAAAAQQAGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAAPGVTPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEQVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSPAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDTLYFSLRYLPVAHGILEY"
     gene            7631..8392
                     /gene="envY"
                     /locus_tag="ACHKTW_001834"
     CDS             7631..8392
                     /gene="envY"
                     /locus_tag="ACHKTW_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator EnvY"
                     /protein_id="HFO6462867.1"
                     /translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLIPHINRNIINDYLLFLNKNLTCVKPWSRLATPVIACHSRTPEVFRLAA
                     NHSKQQPSKPCEAELTRALLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPSLLKKKLKNENTSYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFYGLTPLNYLAKQRQKVMW"
     gene            8904..9857
                     /gene="ompT"
                     /locus_tag="ACHKTW_001835"
     CDS             8904..9857
                     /gene="ompT"
                     /locus_tag="ACHKTW_001835"
                     /EC_number="3.4.23.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease OmpT"
                     /protein_id="HFO6462868.1"
                     /translation="MRAKLLGIVLTTPIAISSFASTETISFTPDNINADISLGTLSGK
                     TKERVYLAEEGGRKVSQLDWKFNNAAIIKGAINWDLMPQISIGAAGWTTLGSRGGNMV
                     DQDWMDSSNPGTWTDESRHPDTQLNYANEFDLNIKGWLLNEPNYRLGLMAGYQESRYS
                     FTARGGSYIYSSEEGFRDDIGSFPNGERAIGYKQRFKMPYIGLTGSYRYEDFELGGTF
                     KYSGWVEASDNDEHYDPGKRITYRSKVKDQNYYSVAVNAGYYVTPNAKVYVEGAWNRV
                     TNKKGNTSLYDHNDNTSDYSKNGAGIENYNFITTAGLKYIF"
     gene            10044..11528
                     /locus_tag="ACHKTW_001836"
     CDS             10044..11528
                     /locus_tag="ACHKTW_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO6462869.1"
                     /translation="MRKIITHFKVVLTLLLPVTVSAQQIQWQSCMASQFNHWFGEEKP
                     SPDLLCGYLSVPLKYTDTGGDASYEKKSQVKLALTKLPAKSKHKGSILIISGGPGLPG
                     INPYINFDWPVTNLRESWDIIGFDPRGVGQSTPTINCRQSDTETQENITEKQQVLNKI
                     NACIHNTGAEVIRHIGSNEAVYDIDRIRQALGDKQLTAVAYSYGTQIAALYAERFPYN
                     VRSIVLDGVVDIDDLEDNFTWQLKQAQSYQETFDRFASWCARTKSCPLSSDRDKAITQ
                     FHELLSKLHHKPLLDSKGENISSDELISLTTDLLLWRSSWPTLATAIRQFSQGIVSNE
                     IETALSAPIASEESSDASGVILCVDQGDEQLTPEERKSRKDALANAFPAINFDNGRSD
                     SPDFCELWPIHSDLNKTRLKNTVLPSGLLFVAHKYDPTTPWINARKMAEKFSSPLLTI
                     NGDGHTLALTGVNLCVDKAVVHHLITPQKIENIYCPGNSEAEIQ"
     gene            complement(11712..11924)
                     /locus_tag="ACHKTW_001837"
                     /pseudo
     CDS             complement(11712..11924)
                     /locus_tag="ACHKTW_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309678.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            12074..12742
                     /locus_tag="ACHKTW_001838"
     CDS             12074..12742
                     /locus_tag="ACHKTW_001838"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFO6462870.1"
                     /translation="MVKLMKKNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHK
                     INHDVFEPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATI
                     LGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVKTKVQGTVVMFSA
                     SGKK"
     gene            complement(12687..12824)
                     /locus_tag="ACHKTW_001839"
     CDS             complement(12687..12824)
                     /locus_tag="ACHKTW_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleocapsid protein"
                     /protein_id="HFO6462871.1"
                     /translation="MAYEPCQGVIIGIMPQHVLSKNVLRLDAIFSLKRKTLLQYLEPW
                     F"
     gene            complement(12797..13381)
                     /locus_tag="ACHKTW_001840"
     CDS             complement(12797..13381)
                     /locus_tag="ACHKTW_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFO6462872.1"
                     /translation="MAFRMSEQPRTIKIYNLLAGTNEFIGEGDAYIPPHTGLPANSTD
                     IAPPDIPAGFVAVFNSDEASWHLVEDHRGKTVYDVASGDALFISELGPLPENVTWLSP
                     EGEFQKWNGTAWVKDAEAEKLFRIREAEETKNSLMQVASEHIAPLQDAVDLEIATEEE
                     TSLLEAWKKYRVLLNRVDTSTAPDIEWPTNPVRE"
     gene            complement(13381..16740)
                     /locus_tag="ACHKTW_001841"
     CDS             complement(13381..16740)
                     /locus_tag="ACHKTW_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFO6462873.1"
                     /translation="MAVKISGVLKDGTGKPVQNCTIQLKAKRNSNTVVVNTLASENPD
                     EAGRYSMDVEYGQYSVILLVEGFPPSHAGTITVYEDSQPGTLNDFLGAMTEDDARPEA
                     LRRFELMVEEVARNASAVAQNTAAAKKSASDASTSAREAATHATDAAGSARAASTSAG
                     QAASSAQSASSSAGTASAKATEASKSAAAAESSKSAAATSAGAAKTSETNAAASQQSA
                     ATSASTATTKASEAATSARDASASKEAAKSSETNASSSASSAASSATAAGNSAKAAKT
                     SGTNAKSSETAAAQSASAAADSKTAAASSASAASTNAGQASASATAAGKSAESAASSA
                     STATTKAGEATEQATAAARSASAAKTSETNAKASETSAESSKTAAASSASSAASSASS
                     ASASKDEATRQASAAKGSATTASTKATEAAGSATAAAQSKSTAESAATRAETAAKRAE
                     DIASAVALEDASTTKKGIVQLSSATNSTSETLAATPKAVKAAYDNAEKRLQKDQNGAD
                     IPDKGRFLSNINVYSKGEVDQKKGMRYVVVNAPAGVQEGKYYPLVIKRNDSHRASRVV
                     ISTPSRTANHRMNNCEFNGFVCAGGWTDRSSYACGMFWAYSSSERAIHSILMSNKGDT
                     VDSVFYIEGGAFPVEAFLEEGLSVTAPASDYVVAETTYKFGATDPYSESVAVNLILDF
                     KQGNGFYSSYPVLSKSDISGNKIYANDEVIVRSQNALRMIAGDYGVIWRNDGANTYLL
                     MTDKGDQYGGWNGLRPFAVNNATGEVTINTPLNSPKGIKGNSDTATKLQTAIKISGVS
                     FDGSTDITLTAAHVAAFARRATDTYADADGGVPWNAESGAYNVTRSGDSYILVNFYTG
                     VGSCRTLQMKAHYRNGGLFYRSSRDGYGFEEDWAEVYTSKNLPPESYPVGAPIPWPSD
                     TVPSGYALMQGQTFDKSAYPKLAAAYPSGVIPDMRGWTIKGKPASGRAVLSQEQDGIK
                     SHTHSASASSTDLGTKTTSSFDYGTKSTNNTGAHTHSVSGSTNSAGAHTHSLANVNTA
                     SANSGAGSASTRLSVVHNQNYATSSAGAHTHSLSGTAASAGAHAHTVGIGAHTHSVAI
                     GSHGHTITVNAAGNAENTVKNIAFNYIVRLA"
     gene            complement(16805..17404)
                     /locus_tag="ACHKTW_001842"
     CDS             complement(16805..17404)
                     /locus_tag="ACHKTW_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFO6462874.1"
                     /translation="MRKLYAAILSAAICLAVSGAPAWASEHQSTLSAGYLHARTNVPG
                     SDDLNGINVKYRYEFTDTLGLVTSFSYAGDKNRQLTRYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGMAYSRVSTFSGDYIRVTDNKGKKHDVLTGSDDGRHSNTSLAW
                     GAGVQFNPTESVAIDIAYEGSGSGDWRTDGFIVGVGYKF"
     gene            complement(17471..20869)
                     /locus_tag="ACHKTW_001843"
     CDS             complement(17471..20869)
                     /locus_tag="ACHKTW_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO6462875.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVEGPVDGLKS
                     VLLNSTPVLDSEGNTNISGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRFTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVDNLPPRPFSIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGSQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTFKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGGTEPRITCNAYLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNAVEVNWIDPNNGWETATELVEDTQAIARYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISIGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGSGNPVSVEVQSVTDGVKVKVSRVPDGVAGYSVWGLKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDQSGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFMLRLTVAADDGSERLVSTARTTETTYRFTQ
                     LALGNYRLTVRAVNAWGQQGDPASVSFRIAAPAAPSRIELTPGYFQITATPHLAVYDP
                     TVQFEFWFSEKRIADIRQVETTARYLGTALYWIAASINIRPGHDYYFYVRSVNTVGKS
                     AFVEAVGRASDDAEGYLDFFKGKITESHLGKELLEKVDLTEDNASRLDEFSKEWKDAS
                     DKWNAMWAVKIEQTKDGKHYVAGIGLSMEDTEEGKLSQFLVAANRIAFIDPANGNETP
                     MFVAQGNQIFMTDVFLKRLTAPTITSGGNPPAFSLTPDGKLTAKNADISGSVNANSGT
                     LNNVTINENCQIKGKLSANQIEGDIVKTVSKSFPRTNSYASGTITVRISDDQKFDRQV
                     MIPPVLFRGGKHENFNSNNQQSYWYSTCRLRVTRNGQEIFNQSTTDAQGVFSSVIDMP
                     AGQGTLTLTFTVSSSGANNWTPTTSISDLLVVVMKKSTAGISIS"
     gene            complement(20930..21502)
                     /locus_tag="ACHKTW_001844"
     CDS             complement(20930..21502)
                     /locus_tag="ACHKTW_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFO6462876.1"
                     /translation="MKTGAEAIRALATQLPMFRQKLSDGWYQVRIAGRDVSTSGLTAQ
                     LHETLPDGAVIHIVPRVAGAKSGGVFQIVLGAAAIAGSFFTAGATLAAWGAAIGAGGM
                     TGILFSLGASMVLGGVAQMLAPKARTPRTQTTDNGKQNTYFSSLDNMVAQGNVLPVLY
                     GEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            complement(21499..22242)
                     /locus_tag="ACHKTW_001845"
     CDS             complement(21499..22242)
                     /locus_tag="ACHKTW_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFO6462877.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVVSTPEGERYFPCVNISGEPE
                     AYFRMSPEDWLQAEMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGTIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHREDDWWRHGQNLYLDNLEAT
                     GLYQVPLSSAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHRAWRASAFTGICNDLAAASTFV"
     gene            complement(22248..22946)
                     /locus_tag="ACHKTW_001846"
     CDS             complement(22248..22946)
                     /locus_tag="ACHKTW_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO6462878.1"
                     /translation="MQDIRQETLNECTRAEQSASVVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQAYPIQGSGFELNGKGTSTRPTLTVSNLYGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLSTPTETDG
                     AVFPGRIMLANTCTWTYRGDECGYNGPAVADEYDQPTSDITKDKCSKCLSGCKFRNNV
                     GNFGGFLSINKLSQ"
     gene            complement(22946..23275)
                     /locus_tag="ACHKTW_001847"
     CDS             complement(22946..23275)
                     /locus_tag="ACHKTW_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO6462879.1"
                     /translation="MKTFRWKVKPGMDVASAPSVRKVRFGDGYSQRAPAGLNADLKTY
                     SVTLSVPRWEATALESFLAEHGGWKSFLWTPPYGYRQIKVTCAKWSSRVSMLRVEFSA
                     EFEQVVN"
     gene            complement(23272..25821)
                     /locus_tag="ACHKTW_001848"
     CDS             complement(23272..25821)
                     /locus_tag="ACHKTW_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO6462880.1"
                     /translation="MAEPVGDLVVDLSLDAARFDEQMARVRRHFSGTESDAKKTAAVV
                     EQSMSRQALAAQKAGISVGQYKAAMRMLPAQFTDVATQLAGGQSPWLILLQQGGQVKD
                     SFGGMIPMFRGLAGAITLPMVGATSLAVATGALAYAWYQGNSTLSDFNKTLVLSGNQS
                     GLTADRMLVLSRAGQAAGLTFNQTSESLSALVKAGVSGEAQIASISQSVARFSSASGV
                     EVDKVAEAFGKLTTDPTSGLTAMARQFHNVTAEQIAYVAQLQRSGDEAGALQAANEAA
                     TKGFDDQTRRLKENMGTLETWADRTARAFKSMWDAVLDIGRPDTAQEMLIKAEAAFKK
                     ADDIWNLRKDDYFVNDEARARYWDDREKARLALEAARKKAEQQSQQDKNAQQQSDTEA
                     SRLKYTEEAQKAYERLQTPLEKYTARQEELNKALKDGKILQADYNTLMAAAKKDYEAT
                     LKKPKQSGVKVSAGDRQEDSAHAALLTLQAELRTLEKHAGANEKISQQRRDLWKAENQ
                     FAVLEEAAQRRQLSAQEKSLLAHKDETLEYKRQLAVLGDKVTYQEHLNALAQQADKFA
                     QQQRAKRAAIEAKNRGLTDRQAARDATEQRLKEQYGDNPLALNNVMSEQKKTWAAEDL
                     LRGSWMAGLKSGWSEWKESATDSMSQVKSAATQTFDGIAQNMAAMLTGSEQNWRSFTR
                     SVLSMMTEILLKQAMVGIVGSIGSAIGGGASASGGTAIQAAAAKFHFATGGFTGTGGK
                     YEPAGIVHRGEFVFTKEATSRIGVGNLYRLMRGYATGGYVGTPGSMADSRSQASGTFE
                     QNNHVVINNDGTNGQIGPQALKAVYDVARKAAMDVVTGQMRDGGLFSGGGR"
     gene            complement(25814..26248)
                     /locus_tag="ACHKTW_001849"
     CDS             complement(25814..26248)
                     /locus_tag="ACHKTW_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFO6462881.1"
                     /translation="MFDGELSFALKLAREMGRPDWRAMLAGMSSTEYADWHRFYSTHY
                     FHDVLLDMHFSGLTYTVLSLFFSDPEMHPLDFSLLNRREADEEPEDDVLMQKAAGLAG
                     GVRFGPDGNEVIPASPDVADMTEDDVMLMTVSEGIAGGVRYG"
     gene            complement(26230..26652)
                     /locus_tag="ACHKTW_001850"
     CDS             complement(26230..26652)
                     /locus_tag="ACHKTW_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFO6462882.1"
                     /translation="MFLKTESFEHNGVTVTLSELSALQRIEHLALMKRQAEQAESDSN
                     RKFTVEDAIRTGAFLVAMSLWHNHPKKTKLPSMNEAVKQIEQEVLTTWPTEAISHAEN
                     VVYRLSGMYEFVVNDAPEQAEDAGPAEPVSAGKCSTVS"
     gene            complement(26668..27408)
                     /locus_tag="ACHKTW_001851"
     CDS             complement(26668..27408)
                     /locus_tag="ACHKTW_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO6462883.1"
                     /translation="MPVPNPTMPVKGAGTTLWVYKGSGDPYANPLSDVDWSRLAKVKD
                     LTPGELTAESYDDSYLDDEDADWTATGQGQKSAGDTSFTLAWMPGEQGQQALLAWFNE
                     GDTRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGRPSMAEDRSTVTA
                     TTGMTVTPASASVVKGQSTTLTVAFQPEGATDKSFRAVSADKTKATVSVSGMTITVKG
                     VAAGKVNIPVVSGNGEFAAVAEINVTAS"
     gene            complement(27416..27811)
                     /locus_tag="ACHKTW_001852"
     CDS             complement(27416..27811)
                     /locus_tag="ACHKTW_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFO6462884.1"
                     /translation="MKHTELRAAVLDALEKHDTGATLFDGRPAVFYEEDFPAIAVYLT
                     GAEYTGEELDSDTWQAELHIEVFLPAQVPDSELDSWMESRIYPVMSDIPALSDLITSM
                     VASGYDYRRDDDAGLWSSADLTYVITYEM"
     gene            complement(27808..28386)
                     /locus_tag="ACHKTW_001853"
     CDS             complement(27808..28386)
                     /locus_tag="ACHKTW_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO6462885.1"
                     /translation="MAIKGLEQAVENLSRISKTAVPGAAAMTINRVASSAISQSASQV
                     ARETKVRRKLVKERARLKRATVKNPQARIKVNRGDLPVIKLGNARVVLSRRRRRKKGQ
                     RSSLKGGGSVLVVGNRRIPGAFIQQLKNGRWHVMQRVAGKNRYPIDVVKIPMAVPLTT
                     AFKQNIERIRRERLPKELGYALQHQLRMVIKR"
     gene            complement(28377..28751)
                     /locus_tag="ACHKTW_001854"
     CDS             complement(28377..28751)
                     /locus_tag="ACHKTW_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000752961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFO6462886.1"
                     /translation="MADFDNLFDAAIARADETIRGYMGTSATITSGEQSGAVIRGVFD
                     DPENISYAGQGVRVEGSSPSLFVRTDEVRQLRRGDTLTIGEENFWVDRVSPDDGGSCH
                     LWLGRGVPPAVNRRRGKGDVWP"
     gene            complement(28763..29161)
                     /locus_tag="ACHKTW_001855"
     CDS             complement(28763..29161)
                     /locus_tag="ACHKTW_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HFO6462887.1"
                     /translation="MTKDELIARLRSLGEQLNRDVSLTGTKEELALRVAELEEELDDT
                     DETAGQDTPLSRENVLTGHENEVGSAQPDTVILDTSELVTVVALVKLHTDALHATRDE
                     PVAFVLPGTAFRVSAGVAAEMTERGLARMQ"
     gene            complement(29203..30228)
                     /locus_tag="ACHKTW_001856"
     CDS             complement(29203..30228)
                     /locus_tag="ACHKTW_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFO6462888.1"
                     /translation="MSMYTTAQLLAANEQKFKFDPLFLRLFFRESYPFTTEKVYLSQI
                     PGLVNMALYVSPIVSGEVIRSRGGSTSEFTPGYVKPKHEVNPQMTLRRLPDEDPQNLA
                     DPAYRRRRIIMQNMRDEELAIAQVEEMQAVSAVLKGKYTMTGEAFDPVEVDMGRSEEN
                     NITQSGGTEWSKRDKSTYDPTDDIEAYALNASGVVNIIVFDPKGWALFRSFKAVKEKL
                     DTRRGSNSELETAVKDLGKAVSYKGMYGDVAIVVYSGQYVENGVKKNFLPDNTMVLGN
                     TQARGLRTYGCIQDADAQREGINASARYPKNWVTTGDPAREFTMIQSAPLMLLADPDE
                     FVSVQLA"
     gene            complement(30284..30616)
                     /locus_tag="ACHKTW_001857"
     CDS             complement(30284..30616)
                     /locus_tag="ACHKTW_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HFO6462889.1"
                     /translation="MTSKETFTHYQPLGNSDPAHTATAPGGLSAKAPAMTPLMLDTSS
                     RKLVAWDGTTDGAAVGILAVAADQTSTTLTFYKSGTFRYEDVLWPEAASDETKKRTAF
                     AGTAISIV"
     gene            complement(30626..31945)
                     /locus_tag="ACHKTW_001858"
     CDS             complement(30626..31945)
                     /locus_tag="ACHKTW_001858"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFO6462890.1"
                     /translation="MTAELRNLPHIASMAFNEPLMLEPAYARVFFCALAGQLGISRLT
                     DAVSGDSLTAGEAPAALALSVDDDGPRQARSYQVMNGIAVLPVSGTLVSRTRALQPYS
                     GMTGYNGIIARLQQAASDPMVDGILLDMDTPGGMVAGAFDCADIIARVRDIKPVWALA
                     NDMNCSAGQLLASAASRRLVTQTARTGSIGVMMAHSNYGAALEKQGVEITLIYSGSHK
                     VDGNPYSHLPDDVRETLQSRMDATRRMFAQKVSAYTGLSVQAVLDTEAAVYSGQEAID
                     AGLADELVNSTDAITVMRDALDARKSRLSGGRMTKETQSTTVSATASQADVTGVVQAT
                     EGENASAAQPDVNAQITAAVAAENSRIMGILNCEEAHGREEQARVLAETPGMTVETAR
                     RILAVAPQSAQARSDTALDRLMQGAPAPLAAGNPASDAVNDLLNTPV"
     gene            complement(31926..33527)
                     /locus_tag="ACHKTW_001859"
     CDS             complement(31926..33527)
                     /locus_tag="ACHKTW_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO6462891.1"
                     /translation="MKTSSIPTLLGPDGMTSLREYAGYHGGGSGFGGQLRAWNPPSES
                     VDAALLPNFTRGNARADDLVRNNGYAANAIQLHQDHIVGSFFRLSHRPSWRYLGIGEE
                     EARAFSREVEAAWKEFAEDDCCCIDVERKRTFTMMIREGVAMHAFNGELFVQATWDTS
                     PSRLFRTQFRMVSPKRISNPNNTGDSRNCRAGVQINDSGAALGYYVSEDGYPGWMPQK
                     WTWIPRELPGGRASFIHVFEPVEDGQTRGANVFYSVMEQMKMLDTLQNTQLQSAIVKA
                     MYAATIESELDTQSAMDFILGANSQEQRERLTGWIGEIAAYYAAAPVRLGGAKVPHLM
                     PGDSLNLQTAQDTDNGYSVFEQSLLRYIAAGLGVSYEQLSRNYAQMSYSTARASANES
                     WAYFMGRRKFVASRQASQMFLCWLEEAIARRVVTLPSKARFSFQEARSAWGNCDWIGS
                     GRMAIDGLKEVQEAVMLIEAGLSTYEKECAKRGDDYQEVFAQQVRETMERRAAGLKPP
                     AWAAAAFESGLRQSTEEEKSDSRAA"
     gene            complement(33524..33730)
                     /locus_tag="ACHKTW_001860"
     CDS             complement(33524..33730)
                     /locus_tag="ACHKTW_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019058 - viral life cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail joining protein"
                     /protein_id="HFO6462892.1"
                     /translation="MTRQEELAAARAALHDLMTGKRVATVQKDGRRVEFTATSVSDLK
                     KYIAELEVQTGMTQRRRGPAGFYV"
     gene            complement(33727..35652)
                     /locus_tag="ACHKTW_001861"
     CDS             complement(33727..35652)
                     /locus_tag="ACHKTW_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFO6462893.1"
                     /translation="MNISNSQVNRLRHFVRAGLRSLFRPEPQTAVEWADANYYLPKES
                     AYQEGRWETLPFQRAIMNAMGSDYIREVNVVKSARVGYSKMLLGVYAYFIEHKQRNTL
                     IWLPTDGDAENFMKTHVEPTIRDIPSLLALAPWYGKKHRDNTLTMKRFSNGRGFWCLG
                     GKAAKNYREKSVDVAGYDELAAFDEDIEQEGSPTFLGDKRIEGSVWPKSIRGSTPKVR
                     GTCQIERAASESPHFMRFHVACPHCGEEQYLKFGDKETPFGLKWTPDDPSSVFYLCEH
                     NACVIRQQELDFTDARYICEKTGIWTRDGILWFSSSGEEIEPPDSVTFHIWTAYSPFT
                     TWVQIVKDWMKTKGDTGKRKTFVNTTLGETWEAKIGERPDAEVMAERKEHYSAPVPDR
                     VAYLTAGIDSQLDRYEMRVWGWGPGEESWLIDRQIIMGRHDDEQTLLRVDEAINKTYT
                     RRNGAEMSVSRICWDTGGIDPTIVYERSKKHGLFRVIPIKGASVYGKPVASMPRKRNK
                     NGVYLTEIGTDTAKEQIYNRFTLTPEGDEPLPGAVHFPNNPDIFDLTEAQQLTAEEQV
                     EKWVDGRKKILWDSKKRRNEALDCFVYALAALRISISRWQLDLSALLASLQEEDGAAT
                     NKKTLADYARALSGEDE"
     gene            complement(35627..36172)
                     /gene="nohD"
                     /locus_tag="ACHKTW_001862"
     CDS             complement(35627..36172)
                     /gene="nohD"
                     /locus_tag="ACHKTW_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HFO6462894.1"
                     /translation="MEVNKKQLADIFGASIRTIQNWQEQGMPVLRGGGKGNEVLYDSA
                     AVIKWYAERDAEIENEKLRREVEELRQASETDLQPGTIEYERHRLTRAQADAQELKNA
                     RDSAEVVETAFCTFVLSRIAGEIASILDGIPLSVQRRFPELENRHVDFLKRDIIKAMN
                     KAAALDELIPGLLSEYIEQSG"
BASE COUNT         8352 a        10264 c         8921 g         8688 t
ORIGIN      
        1 tacagacaag cctggaaagt ggaacataaa ttatcggata ttctattgtt gactatttgt
       61 gccgttattt ctggtgcaga aagttgggaa gatatagagg atttcgggga aacacatctc
      121 gatttcttga agcaatatgg tgattttgaa aatggtattc ctgttcacga tactattgcc
      181 agagttgtat cctgtatcag tcctgcaaaa tttcatgagt gctttattaa ctggatgcgt
      241 gactgccatt catcaaatga taaagacgtc attgcaattg atggaaaaac gctccggcac
      301 tcttatgaca agagtcgccg caggggagcg attcatgtca ttagtgcgtt ctcaacaatg
      361 cacagtctgg tcatcggaca gatcaagacg gatgagaaat ctaatgagat tacagctatc
      421 ccagaacttc ttaacatgct ggatattaaa ggaaaaatca tcacaactga tgcgatgggt
      481 tgccagaaag atattgtaga gaagatacaa aaacagggag gtgattattt attcgcggta
      541 aaaggaaacc aggggcggct aaataaagcc tttgaggaag aatttccgct gaaagaatta
      601 aataatccag agcatgacag ttacgcaatc agtgaaaaga gtcacggcag agaagaaatc
      661 cgtcttcata ttgtttgcga tgtccctgat gaacttattg atttcacgtt tgaatggaaa
      721 ggtctgaaga aattatgcgt ggcagtctcc tttcggtcaa taatagcaga acaaaagaaa
      781 gagcccgaaa tgacggtcag atattatatc agttctgctg atttaaccgc agagaagttc
      841 gccacagcaa tccgaaacca ctggcacgtg gagaataagc tgcactggcg tctggacgtg
      901 gtaatgaatg aagacgactg caaaataaga agaggaaacg ccgcagaatt attttcaggg
      961 atacggcaca tcgctattaa tattttaacg aatgataagg tattcaaggc agggttaaga
     1021 cgtaagatgc gaaaagcagc catggacaga aactatctcg cgtcagtcct tgcggggagc
     1081 gggctttcgt aatcttgccc tgcctgctca cctcctccag gcacttccac tacattatga
     1141 cctgctcggt gccaccgctc cttttcacta tgacgttaac ctcccctcct tgacaccatg
     1201 cttttgtcta cattctacgc aaacggcaac ttttataacc agtgtaaaaa taacgtgccg
     1261 caatagtcat acctattaat ggtaaaaagc tgtcacaatt cataaaaaac cttaatatac
     1321 gccaccctaa acataaccag cgttaatgta aggtttttgt gtggactggc ttcttgatgt
     1381 ttttgcaacc tggctctacg gcttaaaagt aatcgcgata acgttagcgg tcatcatgtt
     1441 catcagcggg ctggacgatt tttttattga tgtcgtctac tgggtacgcc gcattaaacg
     1501 caagttgagt gtttatcgcc gctacccgcg aatgagttac cgcgaactgt ataaaccaga
     1561 tgaaaaaccg ttagcgatta tggttccggc gtggaatgaa acgggcgtca tcggcaatat
     1621 ggccgagctg gcggcgacca cgctcgacta cgaaaactat catatctttg ttggcaccta
     1681 ccccaacgac cccgatactc aacgtgatgt tgacgaagtg tgcgctcgct ttccgaacgt
     1741 gcataaggta gtctgcgcgc gtcctggtcc caccagcaaa gccgactgtc tgaacaacgt
     1801 gctggacgcc atcacccagt ttgaacgtag cgccaatttc gcttttgctg gttttattct
     1861 gcatgacgcc gaagatgtga tttcaccgat ggaattgcgt ctgttcaact atctggtcga
     1921 gcgtaaagat ctgattcaga tcccggtgta tccgttcgaa cgcgaatgga cgcacttcac
     1981 cagcatgact tacattgatg agttttcaga gctgcatggc aaagatgttc cggtgcgtga
     2041 agccctcgcc ggacaagtgc ccagcgcagg cgtcggcacc tgtttcagcc gccgcgccgt
     2101 gaccgctctg ttagctgacg gtgacggtat tgctttcgac gtgcagagtc tgactgaaga
     2161 ttacgacatt ggtttccgcc tgaaagagaa aggtatgacg gaaatttttg tccgttttcc
     2221 ggtggtggac gaagccaaag aacgcgagca gcgcaaattt ttacagcacg cacggacgtc
     2281 aaacatgatc tgcgtgcgcg aatatttccc cgataccttt tcgactgcag ttcgacaaaa
     2341 atctcgctgg atcatcggca ttgttttcca gggctttaaa acccacaaat ggacctccag
     2401 cctgacgctg aactactttc tctggcgcga ccgcaaaggg gcaatcagta actttgtcag
     2461 cttcctcgcg atgctggtga tgctccagct tttgctgttg ctggcgtatg aaagtttgtg
     2521 gcccgatgcc tggcatttcc tttctatttt tagcggcagc gcatggttaa tgaccctgct
     2581 gtggctaaac tttggcttga tggttaaccg catcgtgcag cgggtgattt tcgtcactgg
     2641 ctactacggt ctgacgcagg ggctgttatc tgtcctgcgt cttttctggg gcaacctgat
     2701 taacttcatg gccaactggc gcgcgctaaa acaggtactt caacacggcg atccacgtcg
     2761 cgtcgcgtgg gataaaacaa cgcatgactt ccccagcgtg actggcgata cccgctcgtt
     2821 gcgcccgtta ggccaaattc tgctggaaaa tcaggtcatc actgaagaac aactcgatac
     2881 agcactgcgt aatcgcgtcg aaggtctacg cctgggcggt tcaatgctga tgcaggggct
     2941 gattagcgcc gagcagctgg cacaggcgct ggcagagcaa aacggcgtgg cgtgggaatc
     3001 catcgatgcc tggcagatcc cgtcctcgct gattgccgaa atgccggcct ccgtagcgct
     3061 gcattatgca gtactgccgc tgcgtctgga aaatgatgag ttaattgtcg gcagtgaaga
     3121 tggtattgac ccggtttcgc tggcggccct gacgcgtaaa gtcggacgca aagtgcgtta
     3181 cgtcattgtt ctgcggggac aaattgtcac ggggttacgt cactggtatg cacgccgacg
     3241 cggtcacgat ccgcgggcaa tgttgtacaa tgcggttcag catcagtggc tcacggaaca
     3301 gcagaccggt gaaatctggc ggcaatatgt gccgcatcag ttcctgttcg ccgaaatact
     3361 gaccacgctc ggtcatatta atcgttcagc aattaacgtg ttgttattgc gccatgaacg
     3421 cagttctctg ccgctcggca agtttttggt caccgaaggc gttatcagcc aggaaacgtt
     3481 ggatcgcgtc ctgacaattc aacgcgaatt acaagtttcg atgcaatcac tattactcaa
     3541 agcaggttta aacacagaac aggttgcgca actggagtcc gaaaatgaag gagaataacc
     3601 ttaatcgcgt catcggatgg tctggtttac tgctgacgtc tttattgagt accagcgcac
     3661 tcgcagacaa tatcggcacc agcgcagaag agctggggct gagcgattat cgccattttg
     3721 ttatttatcc ccgtctcgat aaggcgctga aggcacagaa aaataacgac gaagcaaccg
     3781 ccatccgcga atttgaatat atacaccagc aggtgccgga taatattccg ctgactttat
     3841 accttgcgga agcctatcgc cattttggtc atgatgaccg ggcgcggctg ttgcttgagg
     3901 atcaactgaa acgtcaccca ggagatgccc gacttgagcg cagtctggcg gctattccgg
     3961 ttgaagtgaa aagcgttacg actgttgaag aactgcttgc ccagcaaaaa gcgtgcgacg
     4021 ctgcgccgac cctgcgttgt cgcagtgaag tcgggcagaa tgccctgcgg ctggcacaat
     4081 tacctgtcgc cagagcgcaa ctgaacgatg cgacgtttgc tgcatcgcca gaaggaaaaa
     4141 cgctgcgaac cgatctgctg caacgggcaa tctacctgaa acaatggtcc caggcagata
     4201 cgctatacaa tgaagcacgc cagcagaaca cattaagcgc ggcagaacgc cgtcagtggt
     4261 ttgacgtgct tcttgccggg cagctggacg atcggatcct ggcactgcaa tcacagggga
     4321 tcttcaccga tcctcagtca tatattactt acgcgaccgc gctggcttat cgtggcgaaa
     4381 aagcacgcct ccagcattat ctcaatgaaa ataagccgct gtttaccacg gacgcacaag
     4441 agaaaagttg gctctatctg ttatctaaat acagcgccaa ccccgttcag gcattggcga
     4501 attatacggt gcagtttgcc gataaccgcc agtatgttgt tggcgcgacg ctaccggtgc
     4561 tgttaaaaga aggtcagtac gacgcagcgc aaaaactgct cgccaccctc cccgccaatg
     4621 aaatgcttga ggagcgttat gctgtcagcg tggcgacccg taacaaggct gaatctctgc
     4681 gtctggcacg attgctgtat cagcaagaac cggcaaatct tacccgcctg gatcaactga
     4741 cctggcaact gatgcagaac gagcagtcac gcgaagctgc cgatttattg ctgcaacgct
     4801 atcctttcca gggcgatgcg cgtgtcagcc agactttaat ggcgcgactg gcgtctctgc
     4861 tggaaagtca tccttacctg gcaacgccgg cgaaggtggc gattttatcg aaacccttac
     4921 cgctggcgga gcaacgtcag tggcaaagtc agttgccggg tattgcagat aattgcccgg
     4981 caatagttcg cttgctgggc gatatgtcgc cttcctacga tgcttccgcc tggaaccgtc
     5041 tggcaaagtg ttatcgggac acgctacccg gtgtggcgct gtatgcatgg cttcaggccg
     5101 aacaacgaca accgaacgcc tggcaacatc gtgcggtagc ctatcaggcg tatcaggttg
     5161 aggactacgc caccgcactg gcggcctggc agaaaatcag tcttcacgac atgagcaatg
     5221 aggatctgct tgctgctgcc aataccgccc aggcggcagg aaatggtgca gctcgcgatc
     5281 gctggctaca acaggcagaa caacgtggac tgggaaacaa tgccctctac tggtggctgc
     5341 atgcgcaacg ttacattcct ggtcagccgg aactcgcact gagcgatctc acgcgctcaa
     5401 tcaatattgc cccttctgcc aacgcttacg ttgcgcgggc gacaatttat cgccaacgtc
     5461 ataatgtccc ggcggcggtg agtgatttgc gcgccgcgct ggaactggaa ccgaataata
     5521 gcaacatcca ggcagcgctt ggttacgcct tgtgggatag cggtgatatc gcacagtcgc
     5581 gggaaatgct cgaacaggcg cataaagggc ttccggacga tccggcactg atccgacaac
     5641 tggcctacgt gaaccagcgt ctggatgaca tgcctgcgac gcagcactac gcccggctgg
     5701 tgattgatga cattgataat caggcgctga taaccccact gaccccagag caaaatcagc
     5761 aacgcttcaa tttccgccgt ttgcatgagg aggtcggtcg ccgctggacg ttcagtttcg
     5821 attcttctat cggcttgcgt tccggggcaa tgagcaccgc taacaataat gtcggcggcg
     5881 cagcgccagg gaaaagctat cgtagctacg ggcaactgga agccgagtac cgcatcggac
     5941 gcaatatgct gctggaaggc gacctgctct cagtttatag ccgcgtcttt gccgataccg
     6001 gagaaaacgg ggtgatgatg ccggtgaaaa atccgatgtc cggcaccggt ctgcgctgga
     6061 agccgctgcg cgatcagatc tttttcctcg ccgtcgaaca gcagttgccg ctgaacggcc
     6121 aaaatggcgc atccgatacc atgctgcgcg ccagcgcctc attctttaat ggcggcaaat
     6181 acagcgacga atggcacccg aacggttcag gctggtttgc ccaaaacctg tacctcgatg
     6241 cggcgcaata tatccgccag gatattcagg cgtggacggc agattatcgc gtcagctggc
     6301 atcagaaggt agctaacgga cagactattg agccttacgc tcacgttcag gacaacggct
     6361 atcgtgataa aggcactcag ggcgcgcagc ttggcggagt cggggtccgc tggaatatct
     6421 ggaccggcga gacgcactac gacgcctggc cgcacaaagt cagtctcggc gtcgagtatc
     6481 aacatacctt taaggcgatt aatcaacgta acggagagcg caacaacgcg tttctcacca
     6541 ttggagtgca ctggtaaatg cgtaagttca ttttcgtatt gctgacactg cttttggtca
     6601 gccctttttc ctttgcgatg aaaggtatta tctggcaacc acaaaaccga gatagtcagg
     6661 ttaccgatac ccagtggcag gggctgatga gtcagttacg tttgcaaggc ttcgataccc
     6721 tggttttgca atggacccgt tacggcgatg cattcaccca gccagaacag cgcgcgttat
     6781 tgtttaagcg tgccgcagct gcgcaacagg ctggcctgaa gctgattgtc gggctgaacg
     6841 ccgatccgga attttttatg caccagaaac agtcgtccgc agcgctggaa agctatctta
     6901 atcgcctgct ggctgccgat ctccagcaag ccagattatg gagcgccgcg cctggcgtaa
     6961 cgccggatgg ctggtacatc agcgcggaaa ttgacgacct gaactggcgc agcgaagctg
     7021 cccgtcagcc tttgctaaca tggttaaaca acgcgcagcg gctgattagc gatgtttcag
     7081 caaaaccggt ttatatcagt agttttttcg ccggaaacat gtcgcccgat ggctatcgcc
     7141 aactgctgga acaagttaaa gcaaccggcg ttaatgtctg ggtacaggat ggcagcggcg
     7201 tggataaact taccgctgaa cagcgtgaac gttatctaca ggccagcgcc gattgccaaa
     7261 gtcccgcccc tgccagcggc attgtttatg aactgtttgt cgccggcaaa ggcaaaacct
     7321 ttacagcgaa accgaaaccg gatgcagaga tcgcctcgct gttagcgaaa cgttcctctt
     7381 gcggtaaaga cactctctat ttctctctgc gctatttgcc cgtcgcgcac ggcattctcg
     7441 agtattaaat ctcctccagg taagtcgggt acgacctggc ttacctcttt cgctctcgtc
     7501 agaattccat caagattaat tgctaaaaag cgtgctaaag aaagaagatc gttatcggcg
     7561 aacgggccga tcactcatgc aggaagcata gatcctttga gcggagtttc tgatatctga
     7621 ggagtgcgaa atgcaattga gcagcagtga accttgcgtg gtgatcctga ccgaaaaaga
     7681 ggtagaggta agcgtcaata accatgctac gtttaccctt ccgaaaaact acctggccgc
     7741 cttcgcgtgc aacaataacg tcattgaact ctcaacgtta aatcacgtat taatccccca
     7801 catcaaccgt aacatcatca acgattatct gttgttttta aataagaact taacctgtgt
     7861 aaagccctgg tcgcggctgg caaccccggt tatcgcttgt catagccgta caccggaagt
     7921 gttccggcta gccgccaacc acagcaagca gcaacccagc aaaccctgcg aagcggagtt
     7981 gacgcgcgca ttacttttta ccgtattgtc taactttctt gagcaatcgc ggtttatagc
     8041 cctactgatg tatatcttac gcagcagcgt ccgcgacagc gtctgccgca ttattcaaag
     8101 cgatattcag cattactgga atctgcgaat tgtcgccagt tcgctatgtt taagccccag
     8161 cctactcaaa aagaaattaa aaaacgaaaa taccagctat agccagattg tcacagagtg
     8221 tcgtatgcgt tacgccgtac agatgttatt gatggataac aaaaatatca ctcaggtggc
     8281 gcaattatgt ggctatagca gcacgtcgta ctttatctct gtttttaagg cgttttacgg
     8341 cctgacaccg ttgaattatc tcgccaaaca gcgacaaaaa gtgatgtggt gaagggcaaa
     8401 gcggaaacgg ataagacggg cataaatgaa gaagaaatgg cgcgccctgc aggattcgaa
     8461 cctgcggccc acgacttaga agttcctaga acgacatttt aagtcaacaa cttaccacgc
     8521 catctctgcg ctcacacgtc ccactacctc aaaacatgta aagccttgca agccattgtg
     8581 aggccttatg tgtctcagtt ttgtccctct tttttgtact aaaaaacata gcaattgagg
     8641 ataaacctca tgctattttc gcttatatgc ctctaaaggc atggcgctta aatagataaa
     8701 aacaccacaa aagcataaaa aaccacacag taaaactgca atataaaaca ataacagata
     8761 attaaaccaa aaacagatag cgcattgtga taatcattca atactaaaca aaatataaac
     8821 agtggagcaa tatgtaattg actcattaag ttagatataa aaatacatat tcaatcatta
     8881 aaacgattga atggagaact tttatgcggg cgaaacttct gggaatagtc ctgacaaccc
     8941 ctattgcgat cagctctttt gcttctaccg agactatatc gtttactcct gacaacataa
     9001 atgcggacat tagtcttgga actctaagcg gaaaaacaaa agagcgcgtt tatctagctg
     9061 aagaaggagg ccgaaaagtc agtcaactcg actggaaatt caataacgct gcaattatta
     9121 aaggtgcaat taattgggat ttgatgcccc agatatctat tggggctgcg ggctggacta
     9181 ctctcggcag ccgaggtggc aatatggtcg atcaggactg gatggattcc agtaaccccg
     9241 gaacctggac agatgaaagt aggcaccctg atacacaact caattatgcc aacgaatttg
     9301 atctgaatat caaaggctgg ctcctcaacg aacccaatta ccgcctggga ctcatggccg
     9361 gatatcagga aagccgttat agctttacag ccagaggtgg ttcctatatc tacagttctg
     9421 aggagggatt cagagatgat atcggctcct tcccgaatgg agaaagagca atcggctaca
     9481 aacaacgttt taaaatgccc tacattggct tgactggaag ttatcgttat gaagattttg
     9541 agctaggtgg cacatttaaa tacagcggct gggtggaagc atctgataac gatgagcact
     9601 atgacccagg aaaaagaatc acttatcgca gtaaagtcaa agaccaaaat tactattctg
     9661 ttgcagtcaa tgcaggttat tacgtaacac ctaacgcaaa agtttatgtt gaaggcgcat
     9721 ggaatcgggt tacgaataaa aaaggtaata cttcacttta tgatcacaat gataacactt
     9781 cagactacag caaaaatggt gcaggcatag aaaactataa cttcatcact actgctggtc
     9841 ttaagtacat cttttaacga agttaaccag attctctccc cggtggtatt gtataacccc
     9901 ggggaatttg tttacagtta caagtagaca atatccacta attttgtaag tatttaacct
     9961 atgtcataac gtgtaatctc ttgccgcaac acaagacctc aatatattca aattttatct
    10021 ttattccgat attcagagac acaatgcgaa aaataattac tcatttcaaa gttgttttaa
    10081 cgttacttct accagtaacc gtatctgccc agcagataca gtggcaatcc tgtatggcca
    10141 gtcaattcaa ccactggttt ggtgaggaaa aaccgtctcc tgacttacta tgtggttatt
    10201 tgtctgttcc attaaaatat acagacacag gcggagatgc ttcttatgaa aaaaaatcac
    10261 aagtcaaact agcgttgaca aaattgccgg caaaaagcaa gcataaagga agtatcctga
    10321 taataagtgg tggtcccggg ttaccaggca taaatcctta tattaacttt gactggccag
    10381 tcacaaatct tcgtgagtca tgggatatta ttggatttga tcctcgaggc gtcggacagt
    10441 ccactccgac aataaactgc cggcaatcag atacagagac tcaggaaaac ataaccgaaa
    10501 agcaacaagt attaaataaa attaatgcct gtatccataa taccggagcc gaagtcattc
    10561 gccatatcgg ctctaacgag gctgtatacg atattgatcg tattaggcaa gccttggggg
    10621 ataaacaact gacagccgtg gcgtattcgt atggaactca aattgcagcc ttatatgcag
    10681 aacgttttcc ctacaacgta agatctatcg ttcttgatgg agtcgtcgat atcgatgacc
    10741 tggaggacaa cttcacatgg caactcaaac aggcacagag ttatcaggaa acgtttgatc
    10801 gctttgcatc ctggtgtgcg cgtacaaaaa gttgcccgct ttcttcagac agagataagg
    10861 caataactca gttccatgag ctattatcaa aattacatca caaaccttta ttagacagta
    10921 agggagaaaa tatatcttca gatgaactca tatcattaac aacagacctt ctgctatggc
    10981 gttcatcatg gccaaccctt gcaactgcca tacgccagtt ctctcagggg attgtcagta
    11041 atgaaattga aactgcgctc agtgctccga tagcctcaga agagtcaagc gatgcttcgg
    11101 gggtaatcct ctgtgtagat cagggggatg agcaattaac accagaagag cgaaaatccc
    11161 gaaaagacgc tcttgcgaat gccttcccgg ctattaactt tgacaatgga cgttccgatt
    11221 cacctgattt ttgtgaatta tggccaatac atagcgacct gaacaaaact cgcctgaaaa
    11281 atactgttct gccctctggt ttactgtttg tagcacacaa atacgaccca acaacgccct
    11341 ggattaatgc ccgtaagatg gcagagaaat tttccagccc gttactaaca ataaatggtg
    11401 atgggcatac attagctctc accggagtta atttatgtgt agataaagca gttgtacatc
    11461 acctgatcac tccacaaaaa atagaaaata tatactgccc aggaaattct gaagcagaaa
    11521 tacaataatt tcaaaatatt ccgctatttg ctcccaatgc aaaacatata ttgcattggg
    11581 agcatattca tatttttgtt attattgtat gaaatcgtct catgtacacg gtcctttttt
    11641 cgccgaaaac tgatattact ttaatcaaaa gttgacgtgc cagattcgaa gtaaatttat
    11701 aaaatatcaa atcaatatac attttgcacc ttcgagatag taatgactgt tactgtaaca
    11761 ttagttacaa acagcttcat acccagagct actgatgtat caactcgatt caacaatacc
    11821 cgatatttat tccatgcctc cagcaacgat ctttcttcct ccgttgcgat ttccagatct
    11881 acagcatcct gcagtggcgc aatatactca ctgaattcct ggatgtagaa ctgtgtggtg
    11941 acggtcttcc agccattcgg ctcctgctgt atcgaagcat accaggctat ttcaatatcg
    12001 ctatgctgcg gcagcattta accccttgta attcatcgcc ataattgatt taattcacaa
    12061 ataaaactat aacatggtga aattaatgaa aaaaaacaca gatgatgggg ctaaaattta
    12121 cacaccactt accctaaagc tttatgactg gtgggttttg ggagtatcaa atcggcttgc
    12181 atggggatgt cctacaaagg aacaccttct tccacacttt ctggaacatt taggtaacaa
    12241 ccatctggat attggtgttg gaactgggtt ttaccttact cacgtacctg agagtagtct
    12301 gatatcttta atggatttga acgaagctag cctgaacgcg gcatctacaa gggctgggga
    12361 atcaaaaatt aaacataaaa ttaaccatga tgtttttgaa ccttatcccg cggcgttaca
    12421 tggtcaattt gattccattt ccatgtttta ccttcttcac tgcctgcctg gaaatatatc
    12481 tacaaaaagc tgtgtaatac gcaatgcggc gcaggcctta actgacgatg gaactctata
    12541 cggagccaca attcttggcg atggagttgt gcacaatagc ttcggtcaaa aactgatgcg
    12601 catttacaat cagaaaggca tcttttcaaa cacaaaagat tccgaagaag gcttaacaca
    12661 tatactctca gagcatttcg agaatgttaa aaccaaggtt caaggtactg tagtaatgtt
    12721 ttccgcttca gggaaaaaat agcatccaac cgcagcacgt tcttgcttaa gacgtgctgc
    12781 ggcataatcc caatgattac tccctgacag ggttcgtagg ccactcaata tcaggtgcag
    12841 ttgatgtatc aacacggttc agcaacaccc gatacttttt ccaggcttcc agcaatgagg
    12901 tttcttcctc cgttgcgatt tccagatcca cagcatcctg aagtggcgca atatgctcac
    12961 tggctacctg catcaggctg ttttttgttt cttccgcctc tcggatccgg aacagttttt
    13021 ctgcttctgc atctttcacc caggctgtgc cgttccactt ctgaaactcc ccttccggcg
    13081 ataaccaggt aacattttcc ggtaacggac cgagttcaga aataaataac gcgtcccctg
    13141 acgctacgtc ataaaccgtt ttaccccgat ggtcttcaac gagatgccac gatgcctcat
    13201 cactgttgaa aacagccacg aagcctgccg gaatatctgg cggtgcaata tcggtactgt
    13261 ttgctggcag acctgtatga ggcggaatat atgcatcacc ttcaccaata aattcattag
    13321 ttccggccag cagattataa atttttatgg tccgtggttg ttcactcatt ctgaatgcca
    13381 ttatgcaagc ctcacaatgt agttaaatgc gatgtttttg acggtgtttt ccgcgttacc
    13441 agcagcgtta acggtgatgg tgtgtccatg tgagccaatc gcaaccgagt gcgtatgagc
    13501 accaataccg acagtatgtg cgtgtgcacc tgcgcttgca gcagtgccgg acagtgagtg
    13561 ggtatgtgcg ccagcagatg atgttgcata gttttgatta tgcacaacag acaatcttgt
    13621 cgatgcacta ccagcaccgg agttagcact agccgtgttc acgttggcta gtgagtgtgt
    13681 gtgtgctcca gccgagtttg tagagccgct cacactgtgt gtatgtgccc cggtgttatt
    13741 cgtggattta gtgccgtaat caaacgacga tgtggttttc gtccccaaat ccgtactgga
    13801 tgcgctggcg ctgtgggtgt gcgatttaat gccgtcctgt tcctgagata atacggcccg
    13861 accactggca ggtttgccct taatcgtcca gccacgcata tcagggatca cgcctgacgg
    13921 ataagcggct gcaagtttcg ggtaagcaga tttgtcaaaa gtctgcccct gcatcagggc
    13981 atagccagac ggaacggtat ctgatggcca cgggattggt gcgccgactg ggtagctttc
    14041 tggcggaaga tttttcgagg tataaacttc tgcccagtct tcctcaaaac cataaccgtc
    14101 tcttgaagaa cggtagaaca gaccaccatt tctgtaatgc gccttcatct gcaaagtccg
    14161 gcaacttccg actccggtat agaagttaac cagaatatag ctgtcgccag agcgggtgac
    14221 attgtaagcg cctgattcgg cattccaggg aacgccccca tccgcatcgg catacgtatc
    14281 cgttgctctt ctggcaaaag cagcaacatg cgcagcggtt aaagtgatat cagtagaacc
    14341 atcaaacgaa acaccagata tttttattgc tgtctgaagt ttcgtagctg tatcagaatt
    14401 tcccttaatc cccttaggcg aattaagtgg tgtattgatc gtcacttcac ctgttgcgtt
    14461 atttacagca aatggtcgaa gaccgttcca gccaccgtac tggtcgcctt tatcagtcat
    14521 gagcaagtaa gtgtttgctc catcatttcg ccatatgaca ccgtaatcgc ctgcaatcat
    14581 ccttaacgcg ttctgactac ggacaataac ttcatcatta gcgtaaatct tattcccgct
    14641 aatatctgac ttactcaaaa cgggatacga actataaaat ccattacctt gtttaaaatc
    14701 taaaattaaa tttacagcaa ctgattcaga ataaggatca gttgcgccaa atttataagt
    14761 tgtttcagca acaacataat cagaagcagg tgcagtaacg gagagtcctt cttctaaaaa
    14821 agcctctacg ggaaatgccc caccttctat atagaatacg ctgtctacgg tatcgccttt
    14881 attactcatc agaatggaat gaatagcgcg ttccgatgat gaataagccc agaacatacc
    14941 acaggcatag ctactccggt ccgtccagcc gcctgcgcaa acaaaaccat taaactcaca
    15001 gttattcatt ctgtgattag ctgtgcgact tggcgttgaa atgacaacgc gggatgcccg
    15061 atggctgtca ttccttttta tcactaacgg ataatattta ccttcctgga cacccgccgg
    15121 ggcatttacc acaacgtatc gcatcccctt tttctgatcc acttcacctt tgctgtaaac
    15181 attgatgtta ctcaggaagc ggcccttgtc gggaatatca gcaccgttct ggtctttctg
    15241 cagacgtttc tctgcattgt cataggctgc ttttactgcc tttggcgttg ccgccagcgt
    15301 ttcagacgtg ctgttggtcg cactgctgag ctgtactatc ccctttttcg tcgtgctcgc
    15361 atcctcaagc gccacggcgg atgcaatatc ctctgcccgt tttgctgctg tctcggcgcg
    15421 cgttgccgcg gattccgccg tacttttgct ctgagctgcc gccgtcgcac tgccagctgc
    15481 ctctgtcgcc ttcgtggatg ccgtcgtggc gctgcccttc gctgctgacg cctgtctggt
    15541 cgcctcatct tttgaagcag acgccgatga tgccgatgac gccgccgaac tggctgacga
    15601 tgcggcagcc gtttttgagg attctgcgct ggtttccgac gctttcgcgt tcgtttcgga
    15661 tgtcttcgct gcggaagctg acctcgctgc tgccgtggcc tgttcagtgg cttcgccagc
    15721 cttcgttgtg gctgttgaag cggatgatgc ggcgctttct gccgactttc cggcagcggt
    15781 ggcactggct gaggcctgcc cggcatttgt tgacgcggcg ctggcagatg aggcagccgc
    15841 tgtttttgag tctgctgctg cggaggcact ctgtgctgct gccgtctcag aagacttagc
    15901 gttcgtcccg gacgtttttg ccgccttcgc ggaatttcct gccgccgtag ccgaggaagc
    15961 tgcactactg gcgcttgatg atgcgttcgt ttctgatgat tttgccgcct cttttgaagc
    16021 cgacgcatcc ctggctgagg tggcagcttc tgacgctttc gtggtcgcgg tggatgcaga
    16081 agtggcggct gattgttgtg acgctgccgc attcgtttct gacgttttcg ccgcaccggc
    16141 actggtagcc gccgcgcttt ttgaggactc tgcagcggca gcactttttg atgcttcagt
    16201 ggcctttgct gatgccgttc ctgcgctgga agacgctgac tgagccgacg acgcggcctg
    16261 tccggctgac gtgctggctg cgcgtgctga gcctgcagca tcagtcgcat gggttgccgc
    16321 ctcacgggct gatgtgctgg catcgctggc tgacttcttc gcggctgctg tgttctgtgc
    16381 caccgcggac gcgttacgcg ccacctcttc caccatcagt tcaaaacgtc tcagtgcctc
    16441 cggacgggca tcatcctccg tcatggcacc gagaaaatca ttcagcgtac cgggttgaga
    16501 atcttcatac acggtgatgg tcccggcatg tgacggcggg aatccctcca ccaacagaat
    16561 aacgctgtac tgaccgtact caacgtccat actgtaacgc ccggcttcat ccggattttc
    16621 tgaggccagc gtgttcacca ccaccgtgtt gctgttacgt tttgctttca gttggattgt
    16681 gcagttctgt accggttttc ctgtgccgtc tttcagtaca cctgaaatct ttactgccat
    16741 attcacccca caaaaaagcc cgcctgaacc ggcgggctgt cataacactg tgttacctgg
    16801 ctaatcagaa tttataaccg acacccacga tgaaaccgtc agtgcgccag tcaccactgc
    16861 cggagccttc ataagcaata tcaatggcca cggattcggt cgggttaaac tgcacgccag
    16921 ccccccacgc cagagacgtg ttgctgtggc gaccgtcatc acttccggtc agcacatcgt
    16981 gctttttccc cttgttgtca gttacgcgga tataatctcc ggagaaagtc gaaacacggc
    17041 tgtaagccat acccgccatc gcatacgcgc tgaaccattc attcacgcgc acagacggcc
    17101 ccgccatcac gctgaaccag cggttacgca cggaatcttc atgccagcgg gtatcgctgt
    17161 aacgggtaag ctggcgattc ttgtctcctg catagctgaa tgacgtcacc agccccagtg
    17221 tgtccgtaaa ctcataacgg tatttcacgt taatcccgtt aagatcatcg ctgccgggaa
    17281 cgttcgtccg ggcatgaaga taccccgcgc tcagcgtgga ctgatgttca gacgcccatg
    17341 caggcgcacc ggatacggcc agacaaatgg ctgcggacaa aatggcggca taaagtttac
    17401 gcataattac ctctcgcttt tctgcaataa aaaaggcgtc atttctgacg cccgttctgg
    17461 gttataaaat tcagctgata ctgatacctg ctgtggattt tttcatcacc acaaccagca
    17521 gatcgctgat actggttgtt ggtgtccagt tattcgctcc tgatgaagat acggtgaatg
    17581 tcagtgtcag cgtcccctgt ccggcaggca tatctataac tgaggaaaat acgccctgag
    17641 catccgtcgt ggactgatta aaaatctcct gaccattgcg ggtcactctt aaccggcagg
    17701 ttgaatacca gtatgactgt tggttattac tgttgaaatt ctcatgctta ccaccgcgga
    17761 ataacactgg cggtatcatg acctgccggt caaatttctg atcatcactg attcttaccg
    17821 tgatggtgcc actggcataa ctgttcgtgc gggggaaaga cttgctgacc gttttgacaa
    17881 tatcgccttc aatctgattg gctgacagtt tccccttaat ctgacagttc tcattaatcg
    17941 tgacgttgtt gagcgtcccg gagttcgcat tcacactgcc actgatatcc gcatttttag
    18001 cagtcagctt tccgtccggt gtcagggaaa atgccggtgg atttccacca ctggtaatgg
    18061 tgggggccgt caggcgtttc aggaacacgt cggtcatgaa tatctggttg ccctgcgcca
    18121 caaacatcgg cgtttcattc ccgtttgccg ggtcaataaa cgcgatacga ttggcggcaa
    18181 ccagaaactg actcagtttg ccttcctctg tatcctccat gctgaggcca atacccgcga
    18241 cataatgttt gccgtctttg gtctgctcaa ttttgacagc ccacatggca ttccacttat
    18301 cactggcatc cttccactct ttcgaaaact catccagtct gctggcgtta tcctccgtca
    18361 ggtcgacttt ttccagcaac tccttgccga gatgagactc ggttatcttg cctttgaaaa
    18421 aatccaggta accttccgca tcatcgctcg cccgaccgac agcctccacg aatgccgatt
    18481 tgccaacggt gttcacacta cgaacgtaaa aataataatc atggcccggc ctgatattga
    18541 tactggcagc tatccagtac agcgccgtgc caagatagcg ggctgtggtt tcaacctgcc
    18601 tgatatcagc aatccgtttt tccgagaacc agaactcaaa ctgtaccgtc gggtcataaa
    18661 ccgcaagatg cggcgtggcg gttatctgaa aatagcccgg tgtcagctca atccgagacg
    18721 gcgctgccgg tgcggcaatc cggaacgata ccgatgccgg atcgccctgc tgtccccacg
    18781 catttaccgc ccggactgtc agcctgtagt tccccagcgc cagttgcgtg aagcggtatg
    18841 tggtttccgt cgtccgggcc gtgctgacca gccgctcact gccgtcatcc gctgccacgg
    18901 tcaggcgaag cataaagctc acccccttca ccaccttcgg cgtgtcccag cgcgccagca
    18961 cctgatattc cccgctgtct gcggtgactt cggcagtcag gtgctgcacc gctggcggcg
    19021 tgacaccatt caccgtgccg ctctggtcgc cgtcaaagtg cgccccgtta tccacgatgg
    19081 cctctttttc cggtacatgc tgcacggcgg tgatggcata cgtgccgtca tcgttctcac
    19141 ggatactcac gcagcggaac aggcgctggc gcagcgtcgg cagcttcagc ccccatacgc
    19201 tgtatccggc aacgccgtca ggaacacggc tcacttttac cttcacgccg tcggtgacgg
    19261 actggacctc cacgctgacc ggattgccac ttccgtcaac caggcttatc agcgtggtac
    19321 cggaggatgg cagcgtgatt tcacggtcga gcgtcagcgt ccgggtctgg ctgttcacag
    19381 ccagcacgcg cccgccgatg ctgatccccg catagtcatc atcgcagatt tcaataacat
    19441 cacccggcac atggcgaagc ccttcggcac ccacgctgaa atccacggtc tgcgtctcca
    19501 gcagctccgt tttaatcagc cacagcccgg cgcggtgtgc ctgcccccgg ctggtacagc
    19561 caaaggcatc catcttcgta acattacgac cgtaacgggc aatggcctgc gtatcttcaa
    19621 caagctctgt cgccgtctcc cagccgttgt tcgggtcaat ccagttcacc tcaacggcat
    19681 tatggcggtc cttcagggcg ctgaagctgt agcggaacgg cgcgccatca tccggcatca
    19741 ccacattact gcggttatag gtccacacct tatctgatgg ccggtcctgc acgaacgtca
    19801 gcgtctgccc gttccatacc ggcatacagc gcatcgccga gcagaaatca ctgagcacat
    19861 cccacgcctt gcgctgtgtg gtcaggtacg cattacaggt gatgcgcggc tccgtgccgc
    19921 caaagccgtc cggcactgac tggtcgcagt actggccgat aacatacaac gcccatttat
    19981 ccacatccgc cgcaccaaga cgtttcccca tgccgtagcg cggatgggtc agcatatccc
    20041 acagacacca ggccatgttg ttgctgtatg ccggtttaaa cgttccgtcc cagataccgc
    20101 tgtattgccg cgtctgcggg ttataattcg acggcacctg cagaatacgc ccgcgcagat
    20161 gataattacg gctcacctgc tggctgccga actgctccga gtccacctgc acgccgacca
    20221 gtgccgtgtt cgggtagcac tgtttcacat cgataatttc ggtgtatgac gaccagagcg
    20281 ttttgttctg cagctggtct gtggtgctgt ccggcgtcat cctacgcatc cggatactga
    20341 acgggcgcgg cggcaggtta tccaccacca ccgaggccag atactgtgaa gtggttttac
    20401 ccttaatggt gatgtctttt tccgtcaccc agccaccgtt acgttgtatc tgaaccagca
    20461 ggcggacttc cgatggattc ctgtccccct ttgaggtggt ttccaccagt gcctgcacgc
    20521 cgaaagtaaa acgcagacgg tcaatgtttg ccgacgtgat ggtccgggtg atcggcgtgt
    20581 catatttcac ttctgtaccg agcaccgtct cggagccgga ggattcaaat ccctccggcg
    20641 gtgtctgctc ctgctcaccg gcccggaaca ccaccgtgac gccggatata ttggtattcc
    20701 cctcactgtc cagcaccggc gtactgttca gcagcacgct ttttaatcca tccaccggac
    20761 cttcaaccgg cccttcgctg atggcatcga tcacactcag cagctgcgtg gacttcaggt
    20821 tgtccttcgc ttcgcgcggg gtatgcccct tactgctgcc tttacccatt cgtcatgctc
    20881 cataaacgat aaaaccgccc ggaggcggtt tcacataaaa cattttgcat cagcgaccaa
    20941 tcaccacaac ctgaccacca tccccttcgt ctgccgtgct gatctcctga gaaaccacgc
    21001 gtgaccccac gcgcatttca ccgtacagaa ccggcagaac attaccctgg gcaaccatgt
    21061 tatccaggga ggagaaatag gtgttctgtt tgccgttatc cgttgtctgt gtacggggag
    21121 ttctggcttt cggtgccagc atctgcgcca caccaccgag caccatactg gcaccgagag
    21181 aaaacaggat gccggtcata ccaccggccc caatggctgc cccccatgct gcaagggtgg
    21241 ctccggcggt aaagaatgat ccggcaatgg cggcagcccc caggacaatc tggaatacgc
    21301 cacctgactt ggccccggcg actctgggaa caatatgaat cacagcgcca tcaggcagag
    21361 tctcatgtaa ctgcgccgtt aacccggacg tgctgacgtc ccgcccggca atccgtacct
    21421 gataccagcc gtcgctcagt ttctgacgaa acatcgggag ctgtgtggcc agtgcgcgga
    21481 tggcttcagc ccccgttttc acacgaaggt cgatgcggcg gccaaatcgt tgcaaatccc
    21541 cgtaaaggca gatgcgcgcc atgcccggtg acgccagagg gagtgtgtgc gtcgctgcca
    21601 tttgtcggta tacctctctc gtttgctcag ttgttcagga atatggtgca gcagctcgcc
    21661 atcaccacag taaatggcgg catgattcgg caccgatgaa ccaaaacagc acagcagcac
    21721 atcgcccggt tgtgctgatg acaacggcac ctgatacagc cctgtggcct ccagattatc
    21781 cagatagaga ttctgaccgt gacgccacca gtcatcctcg cgatgaaaat ccggcatctc
    21841 aatccccgcc agatgataag catcccggaa cagcgtgtaa cagtccgtca ccccgtgctc
    21901 aaagcgccgc ccggtgagat gcggcacaca gcggaactta tgaatcgtcc cccggcagac
    21961 cagccaccac ggcaaatcac tctgcacctg cagccgccgg tcggcctcac tcagccaggg
    22021 cagaccaccg gggtggctgt ggaccagcgc cacaatctca ccctgcattt ctgcctgcag
    22081 ccagtcttcc ggcgacatac ggaaatacgc ctccggctca ccggagatat tcacgcaggg
    22141 gaaatatctt tccccctccg gcgtgcttac cacgaagccg cacgactccg ctggcgcaca
    22201 tcgccgggcg tgcgccagaa tcgctgattc tgtctgtgtc atgggattta ctgcgaaagt
    22261 ttgttaatgg aaaggaagcc gccaaagttg ccgacattat tgcgaaactt acagccactc
    22321 aggcatttgc tgcatttatc cttcgtgata tcggacgtcg gctggtcata ttcatccgcg
    22381 accgccggac cgttataacc gcactcatca ccgcgatagg tccaggtgca ggtgttggcc
    22441 agcatgatgc gccccggaaa aacggcacca tccgtttcag tcggcgtgga cagtacaaag
    22501 gaggcactga ccgcgctcaa ttcgctgcac tgctcgatgc gccagcggct aatcacctcc
    22561 tgctccggat cggcgtcgct gtttccgttg acgaagttca ccgcatccag aaaacgggca
    22621 taaaccttac gcctgaccac cgttccgccg accagactct gcagatcttc cgccatcccg
    22681 gtgaccatgc catacaggtt agaaaccgtc agcgtggggc gcgtactggt gcctttgcca
    22741 ttcagttcga aaccactccc ctgaatggga tacgcctgat actgtcgccc ctgccaggtg
    22801 accggctcac ctttttcgtt ctgctcatta cagaaaaaat aacgttctcc accaacctct
    22861 gtcagatcga tttcccagag caccacgctg gccgactgct ccgcacgggt gcattcattc
    22921 agtgtttcct gtcggatatc ctgcatcagt tcaccacctg ttcaaactct gcgctgaact
    22981 caacacgcaa catactgacc cgcgacgacc attttgcgca ggtcaccttt atctgccggt
    23041 agccataagg cggcgtccac agaaaggatt tccagccccc gtgctcagcc agaaacgact
    23101 caagcgccgt ggcctcccaa cggggaacag aaagcgtcac gctgtacgtt ttcaggtcgg
    23161 cattcagccc ggcaggcgct cgctgggaat agccatcacc aaagcgcacc tttcttacgg
    23221 aaggggccga agccacatcc ataccgggtt tcactttcca gcggaaggtt ttcatcgtcc
    23281 acctccggag aacagaccac catcgcgcat ctgcccggtc acaacatcca ttgccgcctt
    23341 acgggctacg tcataaacag ccttcagtgc ctgtggccct atctgaccgt tcgtgccgtc
    23401 gttgttaatc accacatggt tattctgctc aaacgtcccg gacgcctgcg accggctgtc
    23461 cgccatgctg cccggtgtac cgacataacc gccggtggca tagccgcgca tcagccggta
    23521 aagatttccc acgccaatcc ggctggttgc ctccttcgtg aagacaaact caccacggtg
    23581 aacaatcccc gctggctcat atttgccgcc ggttcccgta aaccctccgg tcgcaaaatg
    23641 gaatttcgcc gcagctgcct gaatggctgt accgcctgac gctgatgcgc cgccgccaat
    23701 ggcgctgcct atacttccga caatccccac cattgcctgc ttaagcagaa tttctgtcat
    23761 catggacagc acggaacggg tgaagctgcg ccagttctgc tcactgccgg tcagcatcgc
    23821 cgccatattc tgcgcaatac catcaaaggt ctgcgtggca gcacttttaa cctgcgacat
    23881 actgtccgtg gcactctctt tccactcgct ccagccggac ttgaggccag ccatccagct
    23941 cccgcgaagc aggtcttcag ctgcccaggt ctttttctgc tctgacatga cgttatttag
    24001 cgccagcgga ttatcgccat actgctcctt cagacgctgt tccgtggcgt cccgcgctgc
    24061 ctgccggtca gtaagccccc ggtttttcgc ctcaatggct gcccgttttg cccgttgttg
    24121 ctgtgcgaac ttatccgcct gctgcgccag cgcgttcagg tgctcctgat acgtgacctt
    24181 atcgccaagt acagccagct ggcgtttgta ctccagcgtc tcatctttat gcgccagcag
    24241 ggatttctcc tgtgcggata gctggcgacg ttgtgccgcc tcctccagta ccgcgaactg
    24301 attttccgcc ttccacaaat cccggcgctg ctggctgatt ttctcattcg ctccggcatg
    24361 cttctccagc gtccggagtt ctgcctgaag cgtcagcagg gcagcatgag cactgtcttc
    24421 ctgacgatcg cccgcagaca ccttcacgcc ggactgtttc ggctttttca gcgtcgcttc
    24481 ataatccttt ttcgccgccg ccatcagcgt gttgtaatct gcctgcagga ttttcccgtc
    24541 tttcagtgcc ttgttcagtt cttcctgacg ggcggtatat ttctccagcg gtgtctgcag
    24601 ccgttcgtaa gccttctgcg cctcttcggt atatttcagc cgtgatgctt cggtatcgct
    24661 ctgctgctgc gcatttttgt cctgttgact ctgctgttca gccttctttc gggcggcttc
    24721 aagcgcaaga cgggcctttt cacgatcatc ccagtaacgc gcccgcgctt catcgttaac
    24781 aaaataatca tccttgcgca gattccagat gtcgtccgct ttcttaaacg cagcctctgc
    24841 cttaatcagc atctcctgag cggtatcagg acgaccaata tccagcaccg catcccacat
    24901 ggatttgaat gcccgtgctg tcctgtctgc ccaagtttcc agcgtgccca tgttctcttt
    24961 caggcgtcgg gtctggtcat caaacccttt cgttgcggcc tcgttcgccg cctgcaatgc
    25021 cccggcttca tcgccggaac gctgcaactg agcaacatac gcaatctgct ccgccgtcac
    25081 gttatggaac tggcgcgcca tcgccgtcag ccccgacgtc gggtcagtgg tcagcttccc
    25141 gaaggcttca gcgaccttgt ccacctccac gccggatgca gaggagaaac gcgccacact
    25201 ctggctgatg gacgcaatct gagcctcacc gcttaccccc gccttaacca gtgcgctgag
    25261 tgactcgctg gtctggttaa acgtcagccc tgccgcctgc ccggctctgg acaggaccag
    25321 catgcgatct gccgtcagac ctgactgatt accagaaagg accagcgttt tgttgaaatc
    25381 ggacagggtt gagttaccct gataccaggc atacgccagc gcaccggtcg ccaccgccag
    25441 cgaggtggcc ccgaccatcg gcagggtgat cgcaccggca agccccctga acatggggat
    25501 catcccgccg aaggagtcct tcacctgacc accctgttgc agcaggatca gccacggact
    25561 ctgcccgcct gcaagctgcg tggccacgtc ggtgaactgc gcaggcagca tacgcatggc
    25621 ggctttatac tgcccgacgg aaatccccgc tttctgtgca gccagcgcct gccggctcat
    25681 cgactgttca acgactgccg ctgttttttt cgcatcactt tccgtaccgg aaaaatgacg
    25741 cctgactctg gccatctgct cgtcaaatct ggccgcatcc agacttaaat caacgaccag
    25801 atcgcctacc ggttcagcca taccggactc ctcctgcgat cccttctgat actgtcatca
    25861 gcattacgtc atcctccgtc atgtccgcca catccgggga agcggggata acttcattcc
    25921 cgtccgggcc aaaacgaacg cctccggcaa gccctgccgc tttctgcatc agcacatcat
    25981 cttcaggctc ttcgtcagcc tcgcgccggt tcagcagact gaaatccagc ggatgcatct
    26041 ctggatcgct gaaaaacagg ctgagcacag tgtacgtcag cccggaaaag tgcatatcca
    26101 gcagaacatc atgaaaataa tgggtactgt aaaagcggtg ccagtcggca tactccgtgg
    26161 atgacatccc ggcaagcatg gcgcgccagt cgggtcgccc catctcacgc gccagtttca
    26221 gggcaaaact cagctcaccg tcgaacactt tcccgcagaa acaggctctg caggcccggc
    26281 gtcctctgcc tgttcaggag catcattcac cacaaactca tacataccgg acagccggta
    26341 caccacgttt tcagcatgag aaattgcctc tgtgggccag gtggtaagca cttcctgctc
    26401 aatctgttta acggcttcat tcatggaagg cagctttgtc ttcttcggat ggttatgcca
    26461 cagggacatc gccaccagaa acgcgccggt tctgatggcg tcttccacag taaacttccg
    26521 gttgctgtct gactccgcct gttctgcctg ccgtttcatc agggcgagat gctcaatacg
    26581 ctgcagggct gacagttcag aaagcgtgac ggtcacaccg ttatgttcaa atgattcggt
    26641 tttcaggaac atcgctgact ctccggatta actggcggtg acgttgattt ctgcaaccgc
    26701 agcaaactca ccattaccag atacgaccgg aatgttgacc ttgcctgcag caacaccttt
    26761 cacggtgatg gtcataccac tgaccgacac ggtggctttt gttttatccg cagacaccgc
    26821 acggaagctc ttgtcggttg cgccctccgg ctggaatgcc acggtcagcg tggtgctctg
    26881 ccctttcact acggaagcac tggcgggtgt caccgtcatg ccggttgtcg ccgtcaccgt
    26941 gctgcgatct tctgccatcg acggacgtcc cacattggtg accttcaccg tgcgggtgat
    27001 cacttccttc gccgtcaccg ccttaccgat actgctgacc cagccacgga acacatcgac
    27061 cgtgccgttc gggaagcgga ttttataggc acgggtatca ccttcattaa accacgccag
    27121 cagcgcctgc tgcccctgct ctccgggcat ccacgccagc gtgaagctgg tatctccggc
    27181 agatttctgc ccctgcccgg tcgcagtcca gtctgcatct tcatcatcga gatagctgtc
    27241 gtcataggac tcagcggtca gttcgccggg cgtcaggtct ttaacttttg ccagacgcga
    27301 ccagtcaacg tctgaaagcg ggttcgcata agggtcaccg ctccccttat aaacccacag
    27361 ggtggtcccg gcacctttca ccggcattgt aggatttggt acaggcatag cgtcctcaca
    27421 tttcataggt aatgacataa gtcagatcgg ctgaactcca caggcccgca tcatcgtcgc
    27481 gccggtagtc atagccactg gccaccatac tggtgatcaa atctgacagt gccgggatat
    27541 cgctcatcac cggataaatc cgggactcca tccacgaatc cagctctgaa tccggcacct
    27601 gagcaggcag gaaaacttca atatgcagct ccgcctgcca ggtatcgctg tccagctctt
    27661 cgcccgtgta ttcagcgccg gtgagataaa cggcaattgc cggaaaatct tcctcataaa
    27721 aaacagcggg gcgaccatca aaaagcgtcg ccccggtgtc atgcttctcc agtgcatcca
    27781 gtacggctgc acggagttca gtatgtttca tcgctttatt accattctca gttgatgctg
    27841 cagcgcatag cccagctctt tcggaagacg ttcacgccgt atccgttcaa tattctgttt
    27901 aaacgccgtg gtaagcggca ccgccatcgg gattttcacc acatcaatgg ggtaacggtt
    27961 tttcccggcc acacgctgca taacatgcca ccggccattt ttcagttgct gaataaacgc
    28021 gccgggaata cgacggttac ccaccacaag cacgctgccg ccacctttca gggatgaacg
    28081 ctgccccttt ttacgacgcc tgcggcggga caggacaacc cgcgcattac ccagcttgat
    28141 tacgggcaaa tccccccggt taactttgat tctggcctgc ggatttttga ccgtggccct
    28201 tttcagcctg gccctttcct ttaccagttt tcggcgtacc tttgtctcac gggcaacctg
    28261 tgacgcagac tgcgatatcg cggatgaagc aacgcggtta atggtcattg cggcggcacc
    28321 aggcaccgcc gttttgctga tacggctgag gttttcaacg gcctgctcaa gaccttttat
    28381 ggccatacat ccccctttcc gcggcgacgg ttaacggcag gcggtacgcc ccgtccaagc
    28441 cagagatgac aacttccgcc atcatccggc gaaacccgat ctacccagaa attttcctca
    28501 ccgatggtca gcgtgtctcc acgccgcagc tgccgcacct catcagtccg gacaaacagg
    28561 gacgggctgg agccttcaac gcgcacgccc tgtccggcat agctgatatt ttcagggtca
    28621 tcaaaaacac cacgtatcac cgcacctgac tgctcaccgg atgtaatggt ggctgacgtt
    28681 cccatgtacc cgcgtatcgt ttcatcggcg cgggcaatgg cagcatcgaa caggttatcg
    28741 aaatcagcca cagcgcctcc cgttattgca ttctggccag gccgcgctct gtcatttcgg
    28801 ctgccacacc ggcagagaca cgaaacgccg ttcccggcag cacaaatgcc acaggttcat
    28861 cccgcgtggc gtgaagtgca tcagtatgca gcttcaccag tgccacgacc gtgaccagtt
    28921 cagacgtatc cagaatcacg gtatccggct gcgctgatcc cacctcattt tcatgtccgg
    28981 tcagcacatt ttcccggctg agaggggtgt cctgaccggc agtttcatcc gtgtcatcaa
    29041 gctcctcttc cagctctgcc acacggagcg ccagttcttc tttcgtcccc gtcaggctga
    29101 catcacggtt cagttgttca ccaagcgagc ggagacgggc aatcagttca tctttcgtca
    29161 tggactcctc cacagagaaa caatggcccc gaagggccat gattacgcca gttgtacgga
    29221 cacgaactca tcagggtcag ccagcagcat cagcggtgct gactgaatca tggtgaactc
    29281 acgcgccgga tcgccggtgg tcacccagtt tttcgggtaa cgggcagagg cgttaatgcc
    29341 ttcgcgctgt gcgtccgcat cctgaatgca gccataggtg cgcagaccgc gtgcctgagt
    29401 gttccccagc accatcgtgt tgtccggcag gaagttcttt ttgacgccgt tttccacgta
    29461 ctgtccggaa tacacgacga tggccacatc gccatacatc cccttatagg acaccgcttt
    29521 acctaggtct ttcaccgctg tctccagctc ggaattagag ccacgacggg tatccagctt
    29581 ctccttgacg gctttgaagg aacggaacag cgcccagcct ttcggatcga acacgatgat
    29641 attcaccaca ccgctggcgt tcagcgcgta ggcttcgata tcgtcggtcg ggtcatacgt
    29701 ggacttgtca cgcttgctcc actccgtgcc gccggactgc gtgatgttat tctcctcact
    29761 gcggcccata tccacctcaa ccggatcgaa ggcttcaccg gtcatggtgt atttgccctt
    29821 aagcacggca gaaactgcct gcatctcttc gacctgagca atggccagct cttcgtcacg
    29881 catgttctgc atgatgatgc gacggcggcg gtaagccggg tccgccagat tctgcggatc
    29941 ttcatccggc aggcgacgca gggtcatctg cggattcact tcatgcttcg gcttgacata
    30001 tcccggcgta aattcagagg tggagccgcc acgggaacgg ataacctcac cggaaacaat
    30061 cggcgaaacg tacagcgcca tgtttaccag tcccggaatt tgtgagagat agactttctc
    30121 cgtggtgaag ggatagctct cacggaaaaa gagacgcaga aacagcggat caaacttaaa
    30181 tttctgctca tttgccgcca gcagctgggc tgttgtgtac atcgacataa aaaaatcccg
    30241 taaaaaaagc cgcacaggcg gcctttagtg atgaagggta aagttaaacg atgctgattg
    30301 ccgttccggc aaacgcggtc cgttttttcg tctcgtcgct ggcagcctcc ggccagagca
    30361 catcctcata acggaacgtg ccggacttgt agaacgtcag cgtggtgctg gtctggtcag
    30421 cagcaaccgc aagaatgcca acggcagcac cgtcggtggt gccatcccac gcaaccagct
    30481 tacggctgga ggtgtccagc atcagcgggg tcattgcagg cgctttcgca ctcaatccgc
    30541 cgggcgcggt tgcggtatga gccgggtcac tgttgcccag cggctggtaa tgggtaaagg
    30601 tttctttgct cgtcataaac atcccttaca ctggtgtgtt cagcaaatcg ttaacggcat
    30661 cagatgccgg gttacctgca gccagcggtg ccggtgcccc ctgcatcaga cgatccagcg
    30721 cagtgtcact gcgcgcctgt gcactctgtg gtgcaactgc cagaatgcgg cgggccgttt
    30781 ccacggtcat accgggggtt tcggccagca cgcgtgcctg ttcttcgcgt ccgtgagcct
    30841 cctcacagtt gaggatcccc ataatgcgac tgttttctgc cgcaaccgct gcggtgatct
    30901 gcgcgttcac gtccggctgc gcagcgctgg cgttctcgcc ctccgtcgct tgcaccacgc
    30961 cagtaacgtc agcctgcgaa gcagtggctg aaacagttgt tgattgagtc tctttggtca
    31021 ttcgccctcc tgagagacgg gatttacgtg catccagtgc atcacgcatg acggtgatcg
    31081 catcggtact gttaacaagt tcatcagcca gtccggcatc aatggcctcc tgaccgctgt
    31141 acactgcagc ctcggtatcc agcacagcct gcacggacag gccggtatat gccgacacct
    31201 tctgtgcaaa catccggcgg gttgcatcca tccgggactg cagtgtttcc cggacatcat
    31261 ccggtagatg gctgtagggg ttgccatcca ccttatggct gccgctgtaa atcagcgtga
    31321 tttccacgcc ctgtttctcc agcgcagcac cgtaattact gtgagccatc atgacgccga
    31381 tggagcctgt ccgggcggtc tgcgtgacca gacgtcggga ggcggcgctg gcaagcaact
    31441 gacctgcact gcagttcatg tcgttggcca gcgcccatac cggctttatg tcacgcacac
    31501 gggcgatgat gtcagcgcag tcaaatgccc ctgccaccat cccgcctggc gtatccatat
    31561 cgagcagaat gccgtccacc atcgggtcgc tggcagcctg ttgcagacgg gcgataatgc
    31621 cgttgtaacc ggtcatcccc gaatacggct gcagcgcccg cgtccgactg accagcgtgc
    31681 cggacaccgg cagcacggcg atgccgttca tgacctgata actgcgggcc tgtcgtggtc
    31741 cgtcatcatc aacggataac gccagcgccg cgggtgcctc tccggcagtc aggctgtcgc
    31801 cggataccgc atccgtcagg cggctgatcc caagctggcc tgcaagcgca caaaagaaaa
    31861 cccgcgcata ggcgggttca agcatcagcg gctcattaaa ggccatactg gcaatatgcg
    31921 ggagattacg cagctctgct gtcactcttc tcctcctctg ttgattgtcg cagcccggat
    31981 tcaaatgccg cagccgccca ggcgggcggt ttaagaccgg ctgcacggcg ctccatcgtt
    32041 tcacggacct gctgggcaaa aacttcctga tagtcgtcac cgcgttttgc gcactctttc
    32101 tcgtaggtac tcagtccggc ttctatcagc atcaccgctt cctgtacttc tttcagacca
    32161 tcgatggcca tacgaccgga gcctatccag tcgcagttcc cccaggcact tcgggcttcc
    32221 tgaaaactga agcgcgcttt tgaaggtaac gtcaccacgc ggcgagcgat ggcctcttcc
    32281 agccagcaca gaaacatctg gctcgcctga cgggatgcga cgaattttcg ccgccccata
    32341 aagtacgccc acgactcgtt cgcactggcc cgtgccgtgg agtagctcat ctgggcgtaa
    32401 ttccgggaaa gctgctcata cgagacaccc agcccggcag cgatataccg caacagtgac
    32461 tgctcaaaca cggagtagcc gttatccgtg tcctgagccg tctgcaggtt cagtgagtcc
    32521 cccggcatca ggtgcggcac ttttgcgcct cccagacgga ccggtgctgc ggcgtaatac
    32581 gcggcaattt caccaatcca gcccgtcagc ctttcccgct gctcctgact gttcgcgccc
    32641 agaataaaat ccatcgctga ctgcgtatcc agctcacttt caatggtggc ggcatacatc
    32701 gccttcacaa tggcgctctg cagctgcgtg ttctgcagcg tgtcgagcat cttcatctgc
    32761 tccattacgc tgtaaaacac atttgcaccg cgggtctgcc cgtcctccac gggttcaaaa
    32821 acgtgaatga acgaggcgcg cccgccgggt aattcacggg gtatccatgt ccatttctgc
    32881 ggcatccagc caggataccc gtcctcgctg acgtaatatc ccagcgccgc accgctgtca
    32941 ttaatctgca caccggcacg gcagttccgg ctgtcgccgg tattgttcgg gttgctgatg
    33001 cgcttcgggc tgaccatccg gaactgtgtc cggaaaagcc gcgacggact ggtatcccag
    33061 gtggcctgaa cgaacagttc accgttaaag gcgtgcatgg ccacaccttc ccgaatcatc
    33121 atggtaaacg tgcgttttcg ctcaacgtca atgcagcagc agtcatcctc ggcaaactct
    33181 ttccatgccg cttcaacctc gcgggaaaag gcacgggctt cttcctcccc gatgcccaga
    33241 tagcgccagc ttgggcgatg actgagccgg aaaaaagacc cgacgatatg atcctgatgc
    33301 aactggatgg cgttggcggc atagccgtta ttgcgtacca gatcgtctgc gcgggcattg
    33361 ccacgggtaa agttgggcag cagggctgca tccacacttt cactcggtgg gttccacgcc
    33421 cgcaactgcc caccaaatcc gctgccaccg ccgtgataac cggcatattc acgcagcgat
    33481 gtcatgccgt ccggccccag aagggtggga atggaggacg ttttcataca taaaatcctg
    33541 caggtcccct gcgtcgctgt gtcatgccgg tctgcacttc cagctccgca atgtattttt
    33601 tcaggtcaga cacggaagtg gccgtaaact ccactcgccg tccgtctttc tgtaccgttg
    33661 ccacccgttt tcctgtcatc aggtcatgca gtgccgcacg ggcagcggca agttcttcct
    33721 gtcgcgtcat tcatcctctc cggataaggc acgggcgtaa tctgccagtg ttttcttgtt
    33781 ggttgctgca ccatcctctt cctgcaggct cgccagcagt gcactgagat ccagctgcca
    33841 gcgggaaata ctgatgcgca gcgccgccag cgcataaacg aagcagtcga gcgcctcatt
    33901 gcgtcgcttt ttgctgtccc acagtatttt tttcctgcca tccacccatt tttcgacctg
    33961 ctcttcagca gtcagctgct gcgcttcggt cagatcaaaa atatccgggt tattcgggaa
    34021 gtgaacggca ccgggaagcg gttcatcccc ttccggcgtc agtgtgaagc ggttataaat
    34081 ctgctctttc gcggtatccg taccgatttc ggtaaggtaa accccgtttt tgtttcgctt
    34141 acgtggcatg ctggccactg gctttccgta gacggatgcc cctttaatgg ggatcacccg
    34201 gaacagccca tgctttttcg agcgttcata cacaatggtc gggtcaatcc cgccagtatc
    34261 ccagcagata cgggataccg acatttctgc accattccgg cgggtatagg ttttattgat
    34321 ggcctcatcc acacgcagca gcgtctgttc atcgtcgtgg cggcccataa taatctgccg
    34381 gtcaatcagc cagctttcct cacccggccc ccatccccat acgcgcattt cgtagcggtc
    34441 cagctgggag tcgataccgg cggtcaggta agccacacgg tcaggaacgg gcgctgaata
    34501 atgctctttc cgctccgcca tcacctcagc atccggacgt tcgccaattt tcgcttccca
    34561 tgtctcaccg agcgtggtgt tcacgaaggt tttacgtttt cccgtatccc ctttcgtctt
    34621 catccagtct ttgacaatct gcacccaggt ggtgaacggg ctgtacgccg tccagatgtg
    34681 aaaggtcaca ctgtccggcg gctcaatctc ttcaccggat gacgaaaacc agagaatgcc
    34741 atcacgggtc cagatcccgg tcttttcgca gatataacgg gcatcagtaa agtccagctc
    34801 ctgctggcgg atgacgcagg cattatgctc gcagagataa aacacgctgg aggggtcatc
    34861 cggcgtccat ttgaggccaa acggcgtctc tttgtcgcca aatttaagat actgctcctc
    34921 cccgcagtgc gggcaggcaa catgaaaacg cataaaatgc ggggattcac tggctgcacg
    34981 ctcaatctgg caggtgcctc tcactttggg cgtggagcca cggatggact ttggccagac
    35041 cgagccttca atacgtttgt cgccaaggaa cgtcggagag ccttcctgtt caatatcctc
    35101 atcaaaggca gcaagttcat cataacccgc cacatccacc gacttttcac ggtagttttt
    35161 tgccgcttta ccgcccaggc accagaagcc acgaccattg gaaaaacgct tcatagtgag
    35221 cgtgttatcc cggtgctttt tgccatacca cggagccagc gccagcagcg acggaatatc
    35281 gcggatggtc ggctcaacgt gggttttcat aaagttctcg gcatcaccat ccgtcggcaa
    35341 ccagataagg gtgttgcgct gcttatgctc tataaagtag gcataaacac ccagcagcat
    35401 tttggaataa ccgacacggg cagacttcac cacattcacc tcacggatgt agtcgctgcc
    35461 catcgcattc atgatggccc gctgaaaggg cagtgtttcc cagcgccctt cctggtatgc
    35521 ggattctttt gggagatagt aattggcatc cgcccattca acggcggtct gtggctccgg
    35581 cctgaacagt gagcgaagcc cggcgcggac aaaatgccgc agcctgttaa cctgactgtt
    35641 cgatatattc actcagcaac cccggtatca gttcatccag cgcggctgct ttgttcatgg
    35701 ctttgatgat atcccgtttc aggaaatcaa catgtcggtt ttccagttcc ggaaaacgcc
    35761 gctgcaccga cagggggatc ccgtcgagaa tactggcaat ttcacctgcg atccgcgaca
    35821 gcacgaaagt acagaatgcg gtttccacca cttcagcgga gtctctggca tttttcagct
    35881 cctgtgcgtc ggcctgcgca cgcgtaagtc gatggcgttc gtactcaata gtccctggct
    35941 ggagatctgt ctcgctggcc tgccgcagtt cttcaacttc ccggcgcagc ttttcgttct
    36001 caatttcagc atccctttcg gcataccatt ttatgacggc ggcagagtca taaagcacct
    36061 cattaccctt gccaccgcct cgcagaacgg gcattccctg ttcctgccag ttctgaatgg
    36121 tacggatact cgcaccgaaa atgtcagcca gctgcttttt gttgacttcc attgttcatt
    36181 ccacggacaa aaacagagaa aggaaacgac agaggccaaa aagct
//
LOCUS       DAZFQD010000036        35125 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.036, whole genome shotgun sequence.
ACCESSION   DAZFQD010000036 DAZFQD010000000
VERSION     DAZFQD010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35125
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.036"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(34..3936)
                     /gene="hrpA"
                     /locus_tag="ACHKTW_001863"
     CDS             complement(34..3936)
                     /gene="hrpA"
                     /locus_tag="ACHKTW_001863"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HFO6462895.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHGVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DPALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVKPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVAIKLFDNPLEQKQAMWNGLRRLLLLNIPSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDKLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
     gene            4137..4742
                     /gene="azoR"
                     /locus_tag="ACHKTW_001864"
     CDS             4137..4742
                     /gene="azoR"
                     /locus_tag="ACHKTW_001864"
                     /EC_number="1.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HFO6462896.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWREKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            complement(4793..6109)
                     /locus_tag="ACHKTW_001865"
     CDS             complement(4793..6109)
                     /locus_tag="ACHKTW_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2/dual specificity phosphatase
                     family protein"
                     /protein_id="HFO6462897.1"
                     /translation="MITERRNVLLQGAGWLLLLAPFFFFTYGSLNQFTAVQDLNSHDI
                     PSQVFGWETAIPFLPWTIVPYWSLDLLYGFSLFVCSTTFEQRRLVHRLILATVMACCG
                     FLLYPLKFSFIRPEVSGVTGWLFSQLELFDLPYNQSPSLHIILCWLLWRHFRQHLAER
                     WRKVCGGWFLLIAISTLTTWQHHFIDVITGLAVGMLIEWMVPVDRRWNYQKPDQRRIK
                     IALPYVVGAGSCIVLMELMVMIQLWWSVWLCWPVLSLLIIGRGYGGLGAITTGKDSQG
                     KLPPAVYWLTLPWRIGMWLSMRWFCRRLEPVSKITAGVYLGAFPRHIPAQNAVLDVTF
                     EFPRGRATKDRLYFCVPMLDLVVPEEGELRQAVAMLETLREEQGSVLVHCALGLSRSA
                     LVVAAWLLCYGHCKTVDEAISYIRARRSHIVLKEDHKAMLKLWENR"
     gene            complement(6099..7856)
                     /locus_tag="ACHKTW_001866"
     CDS             complement(6099..7856)
                     /locus_tag="ACHKTW_001866"
                     /EC_number="2.1.1.-"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HFO6462898.1"
                     /translation="MENSRIHGEHFFTSSDNTALFYRHWPALQPGAKKVIVLFHRGHE
                     HSGRLQHLVDELAMPDTVFYAWDARGHGQTSGPRGYSPSLARSVQDVDEFVRFAASDS
                     QVGLDEVVVIAQSVGAVLVATWVHDYAPAIRGLVLASPAFKVKLYVPLARPALALWHR
                     LRGLFFINSYVKGRYLTHDRQRVASFNNDPLITRAIAVNILLDLYKTSERIIRDAAAI
                     TLPTQLLISGDDYVVHRQPQIDFYQRLRSPLKELHLLPGFYHDTLGEENRAQAFEKMQ
                     SFISRLYANKSQKFDYQHEDRTGPSADRWRLLSGGPVPLSPVDLAYRFMRKAMKLFGA
                     HSAGLHLGMSTGFDSGSSLDYVYQNQPQGSNAFGRLIDKIYLNSVGWRGIRQRKTHLQ
                     ILIKQAVADLHAKGLAVRVVDIAAGHGRYVLDALANEPAVSDILLRDYSELNVAQGQA
                     MIAQRGMSGRVRFEQGDAFNPEELSALTPRPTLAIVSGLYELFPENEQVKNSLAGLAN
                     AIEPGGILIYTGQPWHPQLEMIAGVLTSHKDGKPWVMRVRSQGEMDSLVRDAGFDKCT
                     QRIDEWGIFTVSMAVRRDN"
     gene            complement(7872..8768)
                     /locus_tag="ACHKTW_001867"
     CDS             complement(7872..8768)
                     /locus_tag="ACHKTW_001867"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFO6462899.1"
                     /translation="MLEKSLATLFALLILATLINHFLLWRLPERKEDEVTLRIRTWWG
                     IVICFSLVISGPRWMTLTFFALISFLALKEYCTLISVHFPRWLYWVIPLNYLFIGFNC
                     FELYLLFIPLAGFLILATGQVFMGDPSGFLHTVSAIFWGWIMTVFALSHAAWLLMLPT
                     TNIQGGALLVLFLLALTESNDIAQYLWGKSCGRRKVVPKVSPGKTLEGLVGGVITTMI
                     ASLIIGPLLTPLNTLQALLAGLLIGISGFCGDVVMSAIKRDIGVKDSGKLLPGHGGLL
                     DRIDSLIFTAPVFFYFIRYCCY"
     gene            complement(8768..9373)
                     /locus_tag="ACHKTW_001868"
     CDS             complement(8768..9373)
                     /locus_tag="ACHKTW_001868"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFO6462900.1"
                     /translation="MTLYQIKPLFQSLLRPTMFWLYKHHVTANHITLAALALSLLTGL
                     LLMLVAQPILFMLLPIVLFIRMALNALDGMLARECNQQTRLGAILNETGDVISDIALY
                     LPFLFLPESNASLVILMLFCTILTEFCGLLAQTINGVRSYAGPFGKSDRALIFGLWGL
                     AVAIYPQWMQWNNLLWSIASILLLWTAINRCRSVLLMSAER"
     gene            complement(9544..11850)
                     /locus_tag="ACHKTW_001869"
     CDS             complement(9544..11850)
                     /locus_tag="ACHKTW_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing bactofilin"
                     /protein_id="HFO6462901.1"
                     /translation="MFKARICGWIGLLPLFMLSLPVQAELRCVANTVDIEPFFSAATA
                     EDKQQVEQAINSSVNLVPFGLSASDWKVHRGDLVVEGNIESNQKLIVLGNLTVKGNIS
                     TFSLSNPWVILGNVTATNIVTDSPLLITGSINASGLVFIDSYYDNPSSIKGSINARGI
                     FINDIIAPVVASSTNSEFMVRASDKNDTENVKKALMIINPDAYYWGLINDEDALKEIF
                     KRSNIRMAGNVCNQMKKEALFRPKPSPELVQELQMLDEGNVAAFEGRDIATFDLAIMR
                     TLPRLKGISANLRKQLINSNDEQTIESMARYMPDNEILELTDQQLGYQPVVLGLLDRE
                     PLSVEIMTRMSRLPDGVGPLNLALRENLPLDIVMTLAKRDWDMIIQELYKDAWLLPES
                     IIDGYIRSDDSSIRQVGAGGQLTYNQAMQLANDSSNNVVTSLAFKLAEMKHHGQLLRM
                     TPQESDKVAAYLYQKFENDDDLIRVLFLALPDNLQFNFVKRMEKKSPAYFCCRDMQVI
                     HSDAALQRLLTRFNDPEGWSNLAKNQYLSTSMKQKIWQRALSHRKNNPKADSAAYETS
                     ADMILSELISHGEVDDQMLLNATALIRLEDWDFLESALVSWDNLPAVVLKELQQNTPR
                     NDIWAKFFLRQENSSRAQVDEALRVYYALDPDALAQLDVLAKQPDRIWWSTLAKSNLT
                     FFKFGALNNRHTPPAVLAAEIDPEWWIVAMNNPRFPVDVLKARLKRDPLLALELVNPE
                     LDLVRQLALNGKTRAIREQAMRKLDELY"
     gene            complement(11914..12774)
                     /gene="pdxI"
                     /locus_tag="ACHKTW_001870"
     CDS             complement(11914..12774)
                     /gene="pdxI"
                     /locus_tag="ACHKTW_001870"
                     /EC_number="1.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 4-dehydrogenase"
                     /protein_id="HFO6462902.1"
                     /translation="MSSNTFTLGTKSVNRLGYGAMQLAGPGVFGPPRDRHVAITVLRE
                     ALALGVNHIDTSDFYGPHVTNQIIREALYPYSDDLTIVTKIGARRGEDASWLPAFSPA
                     ELQKAVHDNLRNLGLDVLDVVNLRVIMGDGHGPAEGSIEASLTVLAEMQQQGLVKHIG
                     LSNVTPTQVAEARKIAEIVCVQNEYNIAHRADDAMIDALARDGIAYVPFFPLGGFTPL
                     QSSTLSDVAASLGATPMQVALVWLLQRSPNILLIPGTSSVAHLRENMAAEKLHLSEEV
                     LSTLDGISRE"
     gene            complement(13005..13595)
                     /gene="paaY"
                     /locus_tag="ACHKTW_001871"
     CDS             complement(13005..13595)
                     /gene="paaY"
                     /locus_tag="ACHKTW_001871"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation protein PaaY"
                     /protein_id="HFO6462903.1"
                     /translation="MPIYQIDGLTPVVPEESFVHPTAVLIGDVILGKGVYVGPNASLR
                     GDFGRIVVKDGANIQDNCVMHGFPEQDTVVEEDGHIGHSAILHGCIIRRNALVGMNAV
                     VMDGAVIGENSIVGASAFVKAKAEMPANYLIVGSPAKAIRELSEQELAWKKQGTHEYQ
                     VLVTRCKQTLHQVEPLREVEPNRKRLVFDENLRPKQ"
     gene            complement(13577..14527)
                     /gene="paaX"
                     /locus_tag="ACHKTW_001872"
     CDS             complement(13577..14527)
                     /gene="paaX"
                     /locus_tag="ACHKTW_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation operon negative
                     regulatory protein PaaX"
                     /protein_id="HFO6462904.1"
                     /translation="MSKLVTFIQHAVNAVPVSGTSLISSLYGDSLSHRGGEIWLGSLA
                     ALLEGLGFGERFVRTALFRLNKEGWLDVSRIGRRSFYSLSDKGLRLTRRAESKIYRAE
                     QPAWDGKWLLLLSEGLDKSTLADVKKQLIWQGFGALAPSLMASPSQKLADVQTLLHEA
                     GVADNVICFEAQIPLALSRAALRARVEECWHLTEQNAMYETFIQSFRPLVPLLKEAAD
                     ELTPERAFHIQLLLIHFYRRVVLKDPLLPEELLPAHWAGHTARQLCINIYQRVAPAAL
                     AFVSEKGETSVGELPAPGSLYFQRFGGLNIEQEAICQFTR"
     gene            complement(14628..15941)
                     /gene="paaK"
                     /locus_tag="ACHKTW_001873"
     CDS             complement(14628..15941)
                     /gene="paaK"
                     /locus_tag="ACHKTW_001873"
                     /EC_number="6.2.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047475 - phenylacetate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase PaaK"
                     /protein_id="HFO6462905.1"
                     /translation="MITNTKLDPIETASVDELQALQTQRLKWTLKHAYENVPMYRCKF
                     DAAGVHPDDFRELSDLRKFPCTTKQDLRDNYPFDTFAVPMEQVVRIHASSGTTGKPTV
                     VGYTQNDIDNWANIVARSLRAAGGTPKDKIHVAYGYGLFTGGLGAHYGAERLGATVIP
                     MSGGQTEKQAQLIRDFQPDMIMVTPSYCLNLIEELERQLGGDASGCSLRVGVFGAEPW
                     TQAMRKEIERRLGITALDIYGLSEVMGPGVAMECLETTDGPTIWEDHFYPEIVNPHDG
                     TPLADGEHGELLFTTLTKEALPVIRYRTRDLTRLLPGTARTMRRMDRISGRSDDMLII
                     RGVNVFPSQLEEEIVKFEHLSPHYQLEVNRRGHLDSLSVKVELKESSLTLTHEQRCQV
                     CHQLRHRIKSMVGISTDVMIVNCGSIPRSEGKACRVFDLRNIVGA"
     gene            complement(15968..17173)
                     /gene="paaJ"
                     /locus_tag="ACHKTW_001874"
                     /gene_synonym="paaE"
                     /gene_synonym="ydbW"
     CDS             complement(15968..17173)
                     /gene="paaJ"
                     /locus_tag="ACHKTW_001874"
                     /gene_synonym="paaE"
                     /gene_synonym="ydbW"
                     /EC_number="2.3.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-oxoadipyl-CoA/3-oxo-5,6-dehydrosuberyl-CoA thiolase"
                     /protein_id="HFO6462906.1"
                     /translation="MREAFICDGIRTPIGRYGGALSGVRADDLAAIPLRELLVRNPRL
                     DAECIDDVILGCANQAGEDNRNVARMATLLAGLPQSVSGTTINRLCGSGLDALGFAAR
                     AIKAGDGDLLIAGGVESMSRAPFVMGKATSAFSRQAEMFDTTIGWRFVNPLMAQQFGT
                     DSMPETAENVAELLKISREDQDSFALRSQQRTAKAQSSGILAEEIVPVVLKNKKGVVT
                     EIQHDEHLRPETTLEQLRGLKAPFRANGVITAGNASGVNDGAAALIIASEQMAAAQGL
                     TPRARIVAMATAGVEPRLMGLGPVPATRRVLERAGLSIHDMDVIELNEAFAAQALGVL
                     RELGLPDDAPHVNPNGGAIALGHPLGMSGARLALAASHELHRRNGRYALCTMCIGVGQ
                     GIAMILERV"
     gene            complement(17173..17595)
                     /gene="paaI"
                     /locus_tag="ACHKTW_001875"
     CDS             complement(17173..17595)
                     /gene="paaI"
                     /locus_tag="ACHKTW_001875"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyphenylacetyl-CoA thioesterase PaaI"
                     /protein_id="HFO6462907.1"
                     /translation="MSHKAWQNAHAMYENDACAKALGIDIISMDEGFAVVTMTVTAQM
                     LNGHQSCHGGQLFSLADTAFAYACNSQGLAAVASACTIDFLRPGFAGDTLTATAQVRH
                     QGKQTGVYDIEIVNQQQKTVALFRGKSHRIGGTITGEA"
     gene            complement(17585..19012)
                     /gene="paaH"
                     /locus_tag="ACHKTW_001876"
     CDS             complement(17585..19012)
                     /gene="paaH"
                     /locus_tag="ACHKTW_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008691 - 3-hydroxybutyryl-CoA
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase PaaH"
                     /protein_id="HFO6462908.1"
                     /translation="MMINVQTVAVIGSGTMGAGIAEVAASHGHQVLLYDISAEALTRA
                     IDGIHARLNSRVTRGKLTAETCERTLKRLIPVTDIHALAAANLVIEAASERLEVKKAL
                     FAQLAEVCPPQTLLTTNTSSISITAIAAEVKNPERVAGLHFFNPAPVMKLVEVVSGLA
                     TAAEVVEQLCELTLSWGKQPVRCHSTPGFIVNRVARPYYSEAWRALEEQVAAPEVIDA
                     ALRDGAGFPMGPLELTDLIGQDVNFAVTCSVFNAFWQERRFLPSLVQQELVIGGRLGK
                     KSGLGVYDWRAEREAVVGLEAVSDSFSPMKVEKKSDGVTEIDDVLLIETQGETAQALA
                     IRLARPVVVVDKMAGKVVTIAAAAVNPDSATRKAIYYLQQQGKTVLQIADYPGMLIWR
                     TVAMIINEALDALQKGVASEQDIDTAMRLGVNYPYGPLAWGAQLGWQRILRLLENLQH
                     HYGEERYRPCSLLRQRALLESGYES"
     gene            complement(19014..19802)
                     /gene="paaG"
                     /locus_tag="ACHKTW_001877"
     CDS             complement(19014..19802)
                     /gene="paaG"
                     /locus_tag="ACHKTW_001877"
                     /EC_number="5.3.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-(1,2-epoxy-1,2-dihydrophenyl)acetyl-CoA
                     isomerase PaaG"
                     /protein_id="HFO6462909.1"
                     /translation="MMEFILSHVEKGVMTLTLNRPERLNSFNDEMHAQLAECLKQVER
                     DDTIRCLLLTGAGRGFCAGQDLNDRNVDPTGPAPDLGMSVERFYNPLVRRLAKLPKPV
                     ICAVNGVAAGAGATLALGCDIVIAARSAKFVMAFSKLGLVPDCGGTWLLPRVAGRARA
                     MGLALLGNQLSAEQAHEWGMIWQVVDDETLADTAQQLARHLATQPTFGLGLIKQAINS
                     AETNTLDTQLDLERDYQRLAGRSADYREGVSAFLAKRSPQFTGK"
     gene            complement(19802..20569)
                     /gene="paaF"
                     /locus_tag="ACHKTW_001878"
     CDS             complement(19802..20569)
                     /gene="paaF"
                     /locus_tag="ACHKTW_001878"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dehydroadipyl-CoA hydratase PaaF"
                     /protein_id="HFO6462910.1"
                     /translation="MSELIVSRQQQVLLLTLNRPAARNALNNALLTQLVNELEAAATD
                     SSISVCVITGNARFFAAGADLNEMAEKDLAATLNDTRPQLWARLQAFNKPLIAAVNGY
                     ALGAGCELALLCDVVVAGENARFGLPEITLGIMPGAGGTQRLIRSAGKSLASKMVLSG
                     ESITAQQAQQAGLVSDVFPSDLTLEYALQLASKMARHSPLALQAAKQALRQSQEVALQ
                     AGLAQERQLFTLLAATEDRHEGISAFLQKRTPDFKGR"
     gene            complement(20566..21636)
                     /gene="paaE"
                     /locus_tag="ACHKTW_001879"
     CDS             complement(20566..21636)
                     /gene="paaE"
                     /locus_tag="ACHKTW_001879"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaE"
                     /protein_id="HFO6462911.1"
                     /translation="MTTFHSLTVAKVEPETRDAVTITFAVPQPLQEAYRFRPGQHLTL
                     KASFDGEELRRCYSICRSYLPGEISVAVKAIEGGRFSRYAREHIRQGMTLEVMVPQGH
                     FGYQPQAERQGRYLAIAAGSGITPMLAIIATTLQTEPESQFTLIYGNRTSQSMMFRQA
                     LADLKDKYPQRLQLLCIFSQETLDSDLLHGRIDGEKLQSLGASLINFRLYDEAFICGP
                     AAMMDETEAALKALGMPDKTIHLERFNTPGTRVKRSVNVQSDGQKVTVRQDGRDREIV
                     LNADDESILDAALRQGADLPYACKGGVCATCKCKVLRGKVAMETNYSLEPDELAAGYV
                     LSCQALPLTSDVVVDFDAKGMA"
     gene            complement(21644..22141)
                     /gene="paaD"
                     /locus_tag="ACHKTW_001880"
     CDS             complement(21644..22141)
                     /gene="paaD"
                     /locus_tag="ACHKTW_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaD"
                     /protein_id="HFO6462912.1"
                     /translation="MQRLATIAPPQVHEIWALLSQIPDPEIPVLTITDLGMVRNVTQM
                     GEGWVIGFTPTYSGCPATEHLIGAIREAMTTHGFTPVQVVLQLDPAWTTDWMTPDARE
                     RLRQYGISPPAGHSCHAHLPPEVRCPRCASVHTTLISEFGSTACKALYRCDSCREPFD
                     YFKCI"
     gene            complement(22156..22902)
                     /gene="paaC"
                     /locus_tag="ACHKTW_001881"
     CDS             complement(22156..22902)
                     /gene="paaC"
                     /locus_tag="ACHKTW_001881"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaC"
                     /protein_id="HFO6462913.1"
                     /translation="MNQLTAYTLRLGDNCLVLSQRLGEWCGHAPELEIDLALANIGLD
                     LLGQARNFLSYAAELAGEGDEDTLAFTRDERQFSNLLLVEQPNGNFADTIARQYFIDA
                     WHVALFTRLMESRDPQLAAISAKAIKEARYHLRFSRGWLERLGNGTDVSGQKMQQAID
                     KLWRFTAELFDADEIDIALSEEGIAVDPRTLRAAWEAEVFAGINEATLNVPQEQAYRT
                     GGKKGLHTEHLGPMLAEMQYLQCVLPGQQW"
     gene            complement(22911..23198)
                     /gene="paaB"
                     /locus_tag="ACHKTW_001882"
     CDS             complement(22911..23198)
                     /gene="paaB"
                     /locus_tag="ACHKTW_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaB"
                     /protein_id="HFO6462914.1"
                     /translation="MSNVYWPLYEVFVRGKQGLSHRHVGSLHAADERMALENARDAYT
                     RRSEGCSIWVVKASEIVASQPEERGEFFDPAESKVYRHPTFYTIPDGIEHM"
     gene            complement(23210..24139)
                     /gene="paaA"
                     /locus_tag="ACHKTW_001883"
     CDS             complement(23210..24139)
                     /gene="paaA"
                     /locus_tag="ACHKTW_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097266 - phenylacetyl-CoA 1,2-epoxidase
                     activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaA"
                     /protein_id="HFO6462915.1"
                     /translation="MTQEERFEQRIAQETAIEPQDWMPDAYRKTLIRQIGQHAHSEIV
                     GMLPEGNWITRAPTLRRKAILLAKVQDEAGHGLYLYSAAETLGCAREDIYQKMLDGRM
                     KYSSIFNYPTLSWADIGVIGWLVDGAAIVNQVALCRTSYGPYARAMVKICKEESFHQR
                     QGFEACMALAQGSEAQKQMLQDAINRFWWPALMMFGPNDDNSPNSARSLAWKIKRFTN
                     DELRQRFVDNTIPQVEMLGMTVPDPDLHFDTESGHYRFGEIDWQEFNEVINGRGICNQ
                     ERLDAKRKAWEEGTWVREAALAHAQKQLARKVA"
     gene            24424..26469
                     /gene="paaZ"
                     /locus_tag="ACHKTW_001884"
     CDS             24424..26469
                     /gene="paaZ"
                     /locus_tag="ACHKTW_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation bifunctional
                     protein PaaZ"
                     /protein_id="HFO6462916.1"
                     /translation="MQQLASFLSGTWQSGRGRSRLIHHAISGEALWEVTSEGLDMAAA
                     RQFAIEKGAPALRAMTFIERAAMLKAVAKHLLSEKERFYALSAQTGATRADSWVDIEG
                     GIGTLFTYASLGSRELPDDTLWPEDELIPLSKEGGFAARHVLTSKSGVAVHINAFNFP
                     CWGMLEKLAPTWLGGMPAIIKPATATAQLTQAMVKSIVDSGLVPEGAISLICGSAGDL
                     LDHLDSQDVVTFTGSAATGQMLRVQPNIVAKSIPFTMEADSLNCCVLGEDVTPDQPEF
                     ALFIREVVREMTTKAGQKCTAIRRIIVPQALVNAVSDALVARLQKVVVGDPAQEGVKM
                     GALVNAEQRADVQEKVNILLAAGCEIRLGGQADLSAAGAFFPPTLLYCPQPDETPAVH
                     ATEAFGPVATLMPAQNQRHALQLACAGGGSLAGTLVTADPQIARQFITDAARTHGRIQ
                     ILNEESAKESTGHGSPLPQLLHGGPGRAGGGEELGGLRAVKHYMQRTAVQGSPTMLAA
                     ISKQWVRGAKVEEDRIHPFRKYFEELQPGDSLLTPRRTMTEADIVNFACLSGDHFYAH
                     MDKIAAAESIFGERVVHGYFVLSAAAGLFVDAGVGPVIANYGLENLRFIEPVKPGDTI
                     QVRLTCKRKTLKKQRSAEEKPTGVVEWAVEVFNQHQTPVALYSILTLVARQHGDFVD"
     gene            26717..28990
                     /gene="tynA"
                     /locus_tag="ACHKTW_001885"
     CDS             26717..28990
                     /gene="tynA"
                     /locus_tag="ACHKTW_001885"
                     /EC_number="1.4.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415904.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0008131 - primary amine oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_process: GO:0009308 - amine metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primary-amine oxidase"
                     /protein_id="HFO6462917.1"
                     /translation="MGSPSLYSARKTALALAVALSFAWQAPVFAHGGEAHMVPMDKTL
                     KEFGADVQWDDYAQIFTLIKDGAYVKVKPGAQTAIVNGQSLALQVPVVMKDNKAWVSD
                     TFINDVFQSGLDQTFQVEKRPHPLNALTADEIKQAVEIVKASADFKPNTRFTEISLLP
                     PDKEAVWAFALENKPVDQPRKADVIMLDGKHIIEAVVDLQNNKLLSWQPIKDAHGMVL
                     LDDFASVQNIINNSEEFAAAVKKRGITDAKKVITTPLTVGYFDGKDGLKQDARLLKVI
                     SYLDVGDGNYWAHPIENLVAVVDLEQKKIVKIEEGAVVPVPMTARPFDGRDRVAPAVK
                     PMQIIEPEGKNYTITGDMIHWRNWDFHLSMNSRVGPMISTVTYNDNGTKRKVMYEGSL
                     GGMIVPYGDPDIGWYFKAYLDSGDYGMGTLTSPIARGKDAPSNAVLLNETIADYTGVP
                     MEIPRAIAVFERYAGPEYKHQEMGQPNVSTERRELVVRWISTVGNYDYIFDWIFHENG
                     TIGIDAGATGIEAVKGVKAKTMHDETAKDDTRYGTLIDHNIVGTTHQHIYNFRLDLDV
                     DGENNSLVAMDPVVKPNTAGGPRTSTMQVNQYNIGNEQDAAQKFDPGTIRLLSNPNKE
                     NRMGNPVSYQIIPYAGGTHPVAKGAQFAPDEWIYHRLSFMDKQLWVTRYHPGERFPEG
                     KYPNRSTHDTGLGQYSKDNESLDNTDAVVWMTTGTTHVARAEEWPIMPTEWVHTLLKP
                     WNFFDETPTLGALKKDK"
     gene            complement(29048..30547)
                     /gene="feaB"
                     /locus_tag="ACHKTW_001886"
                     /gene_synonym="maoB"
                     /gene_synonym="padA"
     CDS             complement(29048..30547)
                     /gene="feaB"
                     /locus_tag="ACHKTW_001886"
                     /gene_synonym="maoB"
                     /gene_synonym="padA"
                     /EC_number="1.2.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415903.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetaldehyde dehydrogenase"
                     /protein_id="HFO6462918.1"
                     /translation="MTEPHVAVLSQVQQFLDRQHGLYIDGRPGPAQSEKRLAIFDPAT
                     GQEIASTADANEADVDNAVMSAWRAFVSRRWAGRLPAERERILLRFADLVEQHSEELA
                     QLETLEQGKSIAISRAFEVGCTLNWMRYTAGLTTKIAGKTLDLSIPLPQGARYQAWTR
                     KEPVGVVAGIVPWNFPLMIGMWKVMPALAAGCSIVIKPSETTPLTMLRVAELASEAGI
                     PDGVFNVVTGSGAVCGAALTSHPHVAKISFTGSTATGKGIARTAADHLTRVTLELGGK
                     NPAIVLKDADPQWVIEGLMTGSFLNQGQVCAASSRIYIEAPLFDTLVSGFEQAVKSLQ
                     VGPGMSPVAQINPLVSRAHCDKVCSFLDDAQAQQAELIRGSNGPAGEGYYVAPTLVVN
                     PDAKLRLTREEVFGPVVNLVRVADGEEALQLANDTEYGLTASVWTQNLSQALEYSDRL
                     QAGTVWVNSHTLIDANLPFGGMKQSGTGRDFGPDWLDGWCETKSVCVRY"
     gene            30783..31688
                     /gene="feaR"
                     /locus_tag="ACHKTW_001887"
     CDS             30783..31688
                     /gene="feaR"
                     /locus_tag="ACHKTW_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator FeaR"
                     /protein_id="HFO6462919.1"
                     /translation="MNPAVDNEFQQWLSQINQVCGNFTGRLLTERYTGVLDTHFAKGL
                     KLSTVTTSGVNLSRTWQEVKGSDDAWFYTVFQLSGQAIMEQDERQVQIGAGDITLLDA
                     SRPCSLYWQESSKQISLLLPRTLLEQYFPHQKPVCAERLDADLPMVQLSHRLLQESMN
                     NPALSETESEAALQAMVCLLRPVLHQRESVQPRRERQFQKVVTLIDDNIREEILRPEW
                     IAGETGMSVRSLYRMFADKGLVVAQYIRNRRLDFCADAIRHAADDEKLAGIGFHWGFS
                     DQSHFSTVFKQRFGMTPGEYRRKFR"
     gene            complement(31860..32189)
                     /locus_tag="ACHKTW_001888"
     CDS             complement(31860..32189)
                     /locus_tag="ACHKTW_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HFO6462920.1"
                     /translation="MMKKTLLLCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVA
                     LKTDAETEKLVTDINAERKASYQQLAKQNNVSVDDIAKLAGQKLVARAKPGEYVQGIN
                     GKWVRKF"
     gene            complement(32194..32379)
                     /locus_tag="ACHKTW_001889"
     CDS             complement(32194..32379)
                     /locus_tag="ACHKTW_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HFO6462921.1"
                     /translation="MKILLAALTSSFMLAACTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            complement(32376..35015)
                     /locus_tag="ACHKTW_001890"
     CDS             complement(32376..35015)
                     /locus_tag="ACHKTW_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HFO6462922.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLAGIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGTVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVVAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWPYAGFPLS
                     GRLGVKVDNWQAGLENALISGRLSVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLSDPTLTFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRDPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGPMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTKINLDNLGLLTMQANITGTSRVDGKSGTVNLNYYHEENIFTLWRSLRFGDNLQA
                     WLEQNARLPGNDCPQGKECEEKQ"
BASE COUNT         8756 a         9482 c         8361 g         8526 t
ORIGIN      
        1 ccgggttttt tctttatcag gcaaatagca gtgttaaccg ctaatctgct ccatcgcctg
       61 caaaatacgc ttatccgaaa taggataagg cgtaccaagt tgttgagcga agtaactaac
      121 gcgcaactct tctatcatcc aacggatctc tttcacgtct tcatcctcac gacgtgcggg
      181 cggcagtttg ttgatccatt gctgccacgc ctgctggacg ttttcgactt tcagcatctg
      241 cgcacggtcg cgatgtggat caaccgccag tttttccagc cgtttttcaa tcgcctgcaa
      301 atatcgcagc gtgtcgccca gccgtttgaa gccgttacca gtgacaaaac cgcgatatac
      361 caacccgccc atctgcgctt taatgtcaga aagccccagc gccatggtca tatccacccg
      421 ccctttcaga cgtttgttga tattgaacac tgccgtaagg atttgctcga cctgcttcgc
      481 aatatccacc accgtgtcgt tcagttcggc acgcactttt tcatgcagcg cagcaaagcc
      541 ttcttccgtc cagaccgggc caccattggc gtcgatcaat ttatccacac cgcaggagat
      601 acagtcgtcg atcagctcca gcactttgcc atacgggtta aagtacagtc ccagcttggc
      661 tttgttcggt aacttttcat gtaaatattt gattggcgat ggaatattca gcagcagtag
      721 acggcgaaga ccgttccaca ttgcctgctt ttgctccagc gggttatcaa acagtttgat
      781 cgccacactg tcgcgctcat ccaccagcgc cggccacgcc ttcactttgt agttgccacg
      841 cttctgttcg tagctttccg gcagctgacc aaaactccag atatgtaagc cgctctgctc
      901 gataccgtca tccgccaccg cggacagcgt ttcctgcact ttgcctttca gcgcatcttt
      961 cagatcctgt aatgagcgcc cttcttttag cttcttattt ttgtcgtcca ccacgcggaa
     1021 ggtgattttc aggtgatcgg gcacctgatc ccagtgccag tcttcgcggt caacggtaac
     1081 gccggtcatc cgccgtaact cgcgctcaag gctgtcgagc aacggtaact ccagcggttt
     1141 gacgcggcct aaaaacgctt cggcatagtt tggcgcgggt acaaaattac ggcgtaccgg
     1201 tttcggcaac gatttaatca gagcaatcac cagttcgcgg cgcagaccgg ggatctgcca
     1261 ttcaaacccg ctttcctcaa cctggttaag taacggcagc ggaatatgta cggtcacacc
     1321 gtcagcatcc gcgccgggct caaactgata gctcaaacgc agcttgagat tgccctgatg
     1381 ccagaagttc gggtaatcca gcttgctgat tttttctgcg ccctctttga tcaacatgct
     1441 tttttcaaag ttgagcaaat caggcgtttc gcggctgact tttttccacc agctatcgaa
     1501 gtggcgcgca gagatcacat cgtggctgat gcgctggtcg tagaactcaa acaacgtttc
     1561 gtcatcaacc agaatatcgc ggcgacgtga tttgtgttcc agctcttcca cttcggcccg
     1621 cagtttcagg ttttcacgga agaatgcgtg acgcgtctgc cagtcacctt ccaccagcgc
     1681 atggcgaata aagagttcac gacataacgc cggatcgatc tggctgtagt tgaccttgcg
     1741 cgcggcaaca atcggcaaac cataaacagt gactttttcc gttgccatca ccgcgccctg
     1801 cgcccgttcc cagtgcggtt cgctgtaggt acgtttaatc aaatgctgag cgacaggctc
     1861 cacccattcc gggtcgatac gcgcagcaat gcgcccccac aggcggctgg tttctaccag
     1921 ttccgccacc attacccatt tcggcggttt tttgaataaa ccagaaccgg ggaagatgga
     1981 gaaacgcgcg ttacgtgcgc cggtatattc ttgtttatcg gcatctttca tgccgatatg
     2041 ggaaagtaaa cccgtcagca acgcaatgtg aatttcgcga tactccgccg gttcgctgtt
     2101 aaccggaatg ccaagttctt tcaccacctg acgcaactgg gtgtagatat cctgccattc
     2161 gcgcacgcgc agatagttga ggtaatcggt acgacacagg cgacggaagg cgttggaaga
     2221 aagcgccttt tgctgctcgc caagataatt ccacagattt acaaacgcga ggaagtcgga
     2281 ttctttgtcg tggaagcgac gatgtttttc gtccgatgcc tgctgtttgt ccatcggccg
     2341 ctcacgcggg tcctgaatgg agagcgcgga cgtgataatc atcgcctcac gcacgcagcc
     2401 atgtttctgc gcttccagca ccatacgcgc cagacgtggg tcgacaggca actgcgagag
     2461 ctggcgaccg agcggcgtca gtttataggc gctggcctgt tcatccgtgg tgatcgcgcc
     2521 cagttcttcg agcagacgca cgccatcctg gatattgcgt ttatccggtg cttcgacaaa
     2581 cgggaacgca gcgatatcgc ccagccccag cgcggtcatc tgcaaaataa ccgaggccag
     2641 gttggtacgc agaatctccg gatcggtaaa ctccgggcgc gaaaggaaat cgtcttcgga
     2701 atagagacga atacagatcc cttcggacac acgcccacag cggcctttac gctgattggc
     2761 agacgcctgc gaaatgggtt caatcggcaa acgctgcact ttggtgcgat agctgtagcg
     2821 gctgatacgt gctgtaccgg ggtcgataac gtatttaatc cccggtacgg tcagcgaggt
     2881 ttccgcgacg ttggtcgcca gcacaatgcg ccgtccgctg tgcgactgga acacgcggtt
     2941 ctgttcgctg ttcgaaagcc gcgcataaag cggcaagatt tcggtatgac gcagatttag
     3001 cttgttcagc gcatcggcgg tatcgcgaat ttcccgctca ccactcataa agatcagaat
     3061 gtcgccaggg ctttcctgac tcagttcgtc tacggcgtca aaaatcgcct gcaactgatc
     3121 gcgctcggtg tcgtcggctt cttcaacaat cggtcgatag cgcacttcca ccggataggt
     3181 ccgaccggag acttcgataa tcggcgcatt attaaagtgg cgcgaaaagc gttccgggtc
     3241 gatagtcgcg gaagtgataa tgatttttag gtcaggacgc cgcggcagca actctttcaa
     3301 atagccgagc agaaaatcga tattcaggct gcgttcgtgt gcttcgtcaa taatgatggt
     3361 gtcgtactgc atcagcaggc ggtcttgctg gatctccgcc agcaggatac cgtcggtcat
     3421 cagcttgacc atcgtgttat cactaacgtg atcgctgaaa cgcactttgt aaccgatgca
     3481 accgcccggc tccgttttca gctcttccgc aatacggttc gccaccgttc ttgccgccag
     3541 gcgacgcggc tgggtatggc cgatcagccc tttaatcccg cgccccagct ccatacagat
     3601 tttcggtaac tgagtcgttt taccagaacc cgtttccccg gcgacgatca ccacctggtg
     3661 atcacgaatc gcttcgagaa tgtcctgttt tttctgacta acaggtaaat tgtcaggata
     3721 agtaatttcc ggtcgtgccg cttcacgcag caggactttc cctgccgctt ggtcaatctc
     3781 tttcgccatc tcctggaaaa tggcctgttg tgcatcagga tttttaacct tcttaacacc
     3841 gtgcagacgc cgagaaaaac gcagtctgtc gcgcagcatc agcgaatcca gccgctgctg
     3901 caaggccgta aaggtcaatt tttgttgttc tgtcataacg ttagtgggca gtagtctgcc
     3961 tgattaattt tcttttaaat gatagatata gagtaccaca tcgcggcttt ctatgcgttg
     4021 ttcaataaat tcgaacatag gcttcgattt attgcgctat ctctgtggca ggattgtgaa
     4081 taaagtgtca gcaagcaacg gggcatcgcc cattcaaaca tctataagga aacaccatga
     4141 gcaaggtatt agttcttaaa tccagcatcc tggcagggta ctctcagtct aatcagttgt
     4201 ccgattattt tgttgaacaa tggcgcgaaa agcactccgc tgatgaaatc accgttcgcg
     4261 acctggctgc aaatccgatt ccggtactgg atggcgaact ggttggtgca ctgcgtccga
     4321 gcgatgcgcc gctgactccg cgtcagcagg aagctctggc gctttccgat gagctgattg
     4381 ccgagctgaa agcccacgac gttatcgtta ttgcggcacc gatgtataac ttcaacatct
     4441 caactcagtt gaaaaactat tttgacctgg ttgcacgcgc aggcgttact ttccgctata
     4501 ccgagaacgg tccggaaggt ctggtaacgg gtaaaaaagc catcgttatt accagccgcg
     4561 gtgggatcca caaagatgga ccaacggacc tggtgacgcc gtatctgtcc acgttcctcg
     4621 gctttatcgg cattaccgat gtgaaatttg tcttcgccga agggatcgca tacggtccgg
     4681 aaatggcagc gaaagcgcag tctgacgcga aagcggccat cgacagcatt gttgctgcat
     4741 aaccggacac gatcttgatg tggactgtag ttcagtccac atctcaatcc acttacctgt
     4801 tttcccataa tttcagcatc gctttgtgat cttccttaag cacaatatgc gaacgtctgg
     4861 ctcgaatata actaatcgct tcatcaacgg ttttacagtg tccgtaacat aacaaccatg
     4921 ccgccactac cagcgcactg cgcgataatc ccaacgcgca atggaccaga acgctgcctt
     4981 gctcttcgcg taatgtttcc agcatcgcca cggcctgtcg gagctccccc tcttccggaa
     5041 ccaccagatc cagcatcggt acacagaaat agagtcgatc ttttgtcgct cgtccccgag
     5101 ggaattcaaa ggtgacgtcc agaaccgcat tctgtgccgg aatatgtcgt ggaaacgccc
     5161 ctaaataaac accagcagta attttgctca ccggctccag gcgacgacaa aaccaacgca
     5221 tagacagcca catcccgatg cgccagggca atgtcagcca gtaaacggcg ggcgggagtt
     5281 tcccctgact atctttccct gttgttatcg cgccaagccc accgtaccca cggccaataa
     5341 tgagtagcga taatactggc caacataacc agactgacca ccataactga atcatcacca
     5401 ttagctccat caacacaatg cacgagcccg cgcctacgac atatggcagt gctattttga
     5461 ttcgacgttg atcaggtttc tgataattcc aacgacgatc gacgggcacc atccattcaa
     5521 tcaacatacc taccgccagc cctgtgatga catcaataaa atgatgctgc caggtcgtca
     5581 gagtagaaat agcgatgagt aaaaaccatc cgccacagac tttacgccac ctctcagcca
     5641 gatgctgacg aaagtgacgc caaagtagcc agcagagaat aatatgcagc gaaggagact
     5701 ggttataagg cagatcaaac agttcaagtt gcgaaaatag ccatcccgtc accccactca
     5761 cttcaggacg gataaaacta aacttcagcg gatagagcaa aaaaccgcag caggccatta
     5821 ccgttgccag aataagccgg tggacaagtc ggcgctgttc gaatgtcgtg ctacaaacga
     5881 acagcgaaaa tccatataaa agatccagac tccagtaagg aacaatagtc cagggaagaa
     5941 aagggatcgc cgtttcccaa ccgaatacct gactggggat atcatggctg ttaaggtcct
     6001 gaaccgcggt gaactgatta agagatccat aggtgaagaa gaaaaacggg gccagcaaca
     6061 ataaccagcc agcgccttgt agcaatacgt tccgacgttc agttatcacg acgcaccgcc
     6121 atcgaaaccg taaaaatacc ccactcatca atccgttgtg tgcatttatc aaatccggca
     6181 tcacgcacga gtgaatccat ctccccttgc gaacgcacgc gcattaccca cggtttacca
     6241 tctttatgac tggttaacac cccggcaatc atctccagtt gagggtgcca cggctgcccg
     6301 gtgtagatga gaatgccgcc cggttcgatg gcattggcaa gacctgcgag tgagtttttt
     6361 acctgctcat tttcgggaaa aagctcatac aggccagaga caatcgccag cgtaggccgc
     6421 ggagttaacg cgctgagttc ctccgggtta aacgcatcgc cctgttcaaa acgcacccgc
     6481 ccagacattc cccgttgggc aatcatcgct tgcccctgtg caacatttaa ctcactgtaa
     6541 tcacgtaaca aaatatcgct tacggcaggc tcgtttgcca gcgcatccag tacatagcgc
     6601 ccatgccctg cggcaatgtc aaccacgcgg acggctaaac ctttggcgtg gagatcggca
     6661 acggcttgtt taatcagtat ttgtaaatgg gttttacgct ggcgaatacc gcgccagcca
     6721 acactgttca ggtagatttt gtcaattaag cgcccgaatg cgttactgcc ttgcggttga
     6781 ttttgataga cataatccag ggaactgcct gaatcaaagc cggtgctcat tccgagatgc
     6841 aggcccgcag agtgcgcccc gaacaatttc atcgcctttc gcataaagcg atacgccaaa
     6901 tcaaccggcg ataatggcac gggtccacct gaaaggagcc gccagcgatc cgctgatggt
     6961 ccagtgcggt cttcatgctg ataatcaaat ttttgtgact tgttagcata taaacgacta
     7021 ataaagcttt gcattttttc aaatgcctgc gccctgttct cttcacccaa cgtgtcgtga
     7081 taaaagcctg gcagcagatg cagctctttc agagggctac gtaatctctg ataaaaatca
     7141 atctgcggtt ggcgatgcac cacatagtca tcgcctgata tcagaagttg cgtggggagc
     7201 gtaatcgccg ccgcatctct aataatacgt tcagacgttt tgtagagatc gagcaagata
     7261 ttaacggcaa tcgcccgtgt gatcagcgga tcattattga aactcgccac ccgttgccga
     7321 tcgtgggtca aatagcgtcc tttcacatag gaattaataa aaaacagacc acgcagacga
     7381 tgccataacg ccagcgcagg acgtgccagc ggtacataca atttaacctt aaaggccgga
     7441 gaagccagca ccagcccgcg aattgcaggc gcataatcat gaacccatgt ggcaaccagc
     7501 actgcgccga cgctttgcgc gatcactacc acctcatcca gtccgacctg gctatcgctg
     7561 gcagcaaaac gaacaaattc atcgacatcc tgcactgaac gcgcaagaga tgggctataa
     7621 ccacgcggcc ccgaagtttg tccatgccct cgggcatccc aggcataaaa aacagtatct
     7681 ggcatcgcca gttcatcaac gagatgttgt agacgaccag aatgttcatg accgcgatga
     7741 aataagacga tgaccttttt agccccgggc tgtaaagcgg gccagtgccg ataaaacaac
     7801 gctgtattat cactgctggt aaaaaaatgt tccccatgga tgcgtgaatt ttccattttc
     7861 tctattttcc ttcagtaaca gcagtagcga ataaaataaa aaaataccgg agcggtgaaa
     7921 attaatgagt caatcctgtc aaggagtccg ccatgtcctg gtaatagttt cccactatct
     7981 ttaacaccaa tatctcgttt gatggctgac atcacgacat cgccgcaaaa accactaata
     8041 ccaattaaca aacccgctaa taatgcctgt aatgtattta gcggtgtcag caacggtcca
     8101 ataatcagtg acgcaatcat ggtggtgatt acgcccccca ccagaccttc cagcgttttt
     8161 cccgggctga ctttggggac cacttttctt ctgccgcagg atttccccca taaatactgt
     8221 gcaatatcgt ttgactcggt taatgccaaa agaaataaca ccagtaacgc cccgccctgg
     8281 atatttgtgg ttggcaacat taataaccag gcggcatgac tcaaggcgaa aacggtcatt
     8341 atccagcccc aaaaaatggc gctcacggta tgcagaaaac cggaggggtc ccccataaaa
     8401 acttgcccgg tggctaatat tagaaaccca gccaaaggta taaataatag atacagctca
     8461 aagcaattaa aaccgataaa caaatagtta agaggaataa cccaatataa ccaccgcgga
     8521 aaatgtacag atataagcgt acaatattct ttcaatgcca gaaaacttat cagcgcaaaa
     8581 aacgtcaacg tcatccagcg tggacctgaa atcaccagtg aaaaacaaat gacgatgccc
     8641 caccaggtac gaatacgtaa tgtaacttca tcctcttttc tctccggtaa ccgccatagc
     8701 agaaaatggt ttatcagcgt tgctaaaatt aacaacgcga aaagtgttgc cagagatttt
     8761 tccagcatta tctttcagcg ctcataagaa gcacactccg acaacgatta atcgcagtcc
     8821 agagaagcag aattgaagca atgctccata aaagattatt ccactgcatc cactgtggat
     8881 aaatggcaac ggccagaccc cacagaccaa atattaacgc gcgatcgctt ttgccaaatg
     8941 gaccggcata actgcgaaca ccgttaatcg tctgcgcgag taaaccgcaa aactcggtca
     9001 atatggtgca aaataacatg agtatcacga gtgatgcgtt actttccggt aaaaataaaa
     9061 atggtaaata gagcgcaata tcggaaataa catcaccagt ctcattcaaa attgccccaa
     9121 gccgtgtttg ctgattgcac tcacgcgcca acatgccatc cagcgcattg agcgccatac
     9181 ggataaaaag cacgatgggc aatagcataa agaggatggg ttgtgccacc aacatcaaca
     9241 gcaatccggt aagaagagaa agcgccagtg cagcaagagt gatgtgattc gctgtaacgt
     9301 ggtgcttgta aagccaaaac atcgtcggcc ttaacagcga ctgaaagagc ggttttatct
     9361 ggtagagcgt cattatcaat ccttgataat tcggtttgcg ctatggtgca tcaattcccc
     9421 tgacggagat atcgcaacac gccgcttcat taaaaattca gaatatatca aggtattggg
     9481 gactcactgt caggaataat tatacccaat taatcaaagg gttaagaaat attacgttgt
     9541 ggatcaatac aactcatcaa gttttctcat cgcttgttcc cgtattgcgc gcgtcttacc
     9601 gttaagcgcc agctggcgga ctaaatccag ttcagggtta acaagctcta acgccagcaa
     9661 aggatcacgt ttcagcctcg cctttaatac atcaactgga aaacggggat tattcatcgc
     9721 cacaatccac cactcgggat caatttctgc cgccagtact gcaggcggtg tgtggcggtt
     9781 gttaagtgcg ccgaacttga aaaatgtgag attgcttttc gccagtgtac tccaccatat
     9841 acgatccggt tgttttgcca gtacatccag ttgtgctaac gcatcggggt cgagtgcata
     9901 atagacgcgt aatgcttcat caacctgcgc acgggagctg ttttcctgcc tcagaaaaaa
     9961 cttcgcccaa atatcattgc gtggcgtatt ttgctgtaat tccttgagga caacagcggg
    10021 taaattatcc caactaacta atgcactctc taaaaagtcc cagtcttcca aacgtatcag
    10081 ggcggtggca tttaatagca tctgatcatc aacttcaccg tggctgataa gctcagacaa
    10141 aatcatatca gcgctggttt catacgcagc tgaatctgct ttcggattat ttttccgatg
    10201 cgataaagca cgttgccaaa ttttttgttt catcgacgtg ctcagatact gatttttcgc
    10261 cagattactc cacccctcag gatcgttgaa acgtgtcaat aaccgttgta gagcagcgtc
    10321 agagtgaatt acctgcatat cccgacagca aaagtaggca ggggattttt tctccatcct
    10381 tttaacaaaa ttaaactgta agttatctgg caatgctaaa aataacacac gtattagatc
    10441 atcgtcattc tcgaattttt ggtataaata ggctgcaact ttgtcactct cttgtggcgt
    10501 catccgcaat aattgaccat ggtgtttcat ctctgccagc ttgaacgcta agcttgtgac
    10561 aacattgttt gatgaatcgt tcgccaactg cattgcctga ttgtaggtga gttgtcctcc
    10621 agcaccgacc tgacgaatag aggaatcatc actgcggata tagccatcaa taatggattc
    10681 aggcaataac cacgcatctt tataaagctc ctgaatgatc atatcccaat ctcttttcgc
    10741 cagagtcatt acgatatcca gagggagatt ttcacgtagc gcaagattca acggaccgac
    10801 accatcaggt agacgactca ttcgcgtcat tatttcgaca gagagcggtt cacggtcgag
    10861 caaccccaga acaacaggct ggtaacccaa ttgctgatcg gtcaactcca gaatctcatt
    10921 gtcaggcata tatctcgcca tactttcaat cgtttgttca tcattgctat taataagttg
    10981 tttgcgtagg ttagctgaaa ttcctttaag ccttgggaga gttcgcatta tggcaagatc
    11041 aaatgtcgca atatctcgtc cttcaaatgc agcaacgttg ccttcatcca gcatttgcaa
    11101 ttcctgcact aactcaggag aaggcttagg gcgaaacagc gcttcttttt tcatctgatt
    11161 acagacattc cctgccatgc gaatattgct tcgcttaaaa atttctttca gagcatcttc
    11221 atcatttatt agaccccaat aatatgcatc aggatttatt atcatcagcg cttttttgac
    11281 attttcagtg tcatttttgt cactcgcacg gaccatgaat tcactatttg tcgaagacgc
    11341 aacaacaggt gcaattatgt cattgataaa tattccacgc gcattaatac tccccttaat
    11401 cgaagacgga ttatcgtaat atgagtcgat aaataccagt ccacttgcat ttatcgaacc
    11461 ggtaattaac agtggtgaat ccgtaactat attcgttgcc gtcacgttac cgagaataac
    11521 ccatggatta gaaagagaga aagtggaaat attacctttg actgtcaaat taccaagaac
    11581 aatcaatttt tgattactct ctatattacc ttctaccact aaatcgccac gatgcacttt
    11641 ccagtccgat gcagataaac cgaaggggac aagattcacg ctactgttga tagcttgttc
    11701 aacttgttgt ttatcttcag cggtagccgc agaaaaaaat ggttcaatat caactgtatt
    11761 tgcaacacat cgaagttccg cctgtactgg aagactcagc ataaacaggg gaagtaaacc
    11821 tatccagccg caaatcctgg ccttaaacat atgcactcca tgccattaaa aaagaaacaa
    11881 aagggaaatg catctttccc ttttgtatat tcgttattct cgcgaaatac catccaacgt
    11941 agacaacact tcctcagaaa gatgcaattt ttcagcagcc atattctccc gtaaatgcgc
    12001 aacagaagac gtccctggga tcagcaaaat attcggtgaa cgttgtaaca gccacaccag
    12061 cgccacctgc atgggtgttg cacccaggct cgcagcaaca tccgacagcg tagaggattg
    12121 cagcggtgta aagcccccga gcgggaagaa cggcacgtag gcaatgccgt cacgagccaa
    12181 agcatcaatc attgcgtcat cagcacgatg agcaatgttg tattcgttct gcacacagac
    12241 gatttcggca atcttgcgcg cctctgcaac ctgcgtcggt gtgacgttgc tcaggccaat
    12301 atgttttacc aggccttgtt gttgcatctc tgccagcacg gtcaggctgg cttcaatcga
    12361 tccttccgct gggccatgac catcccccat aataacgcgc aggttaacca catccagtac
    12421 gtccaggccg agattacgta gattatcgtg caccgctttt tgcaactctg ccggagaaaa
    12481 ggcgggcaac caggaagcgt cctctccacg ccgcgcacca attttagtga caattgtcag
    12541 gtcgtcagag taaggataaa gcgcttcgcg gataatctga ttggtgacgt gcggaccata
    12601 aaagtcgctg gtatcaatgt gattgacgcc caatgccagc gcctcgcgca gcacggttat
    12661 agcgacgtgg cgatctcttg gggggccaaa aaccccagga cctgccagtt gcatcgcgcc
    12721 ataaccaaga cggttaacgg attttgtacc gagagtaaat gtattgctgc tcatcgcacc
    12781 ctgccctgta ataaaacgga ttaagaaaaa tcagtataga aacgaaaaga gataaataat
    12841 gcgagggcgt aaacaagatt actggatgag ttgctctatt gtcctgaaaa acacaaaaca
    12901 aaacgcgtaa agcgccgcat ccggcaataa aaaaaccgca acctgtatca gcgttgcggt
    12961 ttaggtatca catgctaaaa attagaatgt tcagtatctg gacgttactg tttcggtcgc
    13021 agattctcat caaataccag acgtttcctg ttcggttcaa cttcccgcaa tggctcaact
    13081 tgatgcaacg tctgcttaca acgagtcacc agcacctgat actcatgcgt accctgcttt
    13141 ttccatgcca gctcctgctc actgagttca cgaatcgctt tcgccgggct gccgacaatc
    13201 aggtaattgg caggcatttc tgctttggct ttcacaaatg cggatgcgcc aacaatgctg
    13261 ttctcgccaa tcaccgcgcc gtccatcact accgcgttca ttcccactaa tgcattgcgg
    13321 cggataatgc aaccatgaag gatagcgcta tgaccaatat gtccatcttc ttccacgaca
    13381 gtatcctgct cgggaaaacc gtgcataacg caattatcct gaatgttcgc gccatctttc
    13441 accacgatac gaccaaaatc gccacgcagg ctggcatttg gcccaacgta aacgcccttg
    13501 cctagaataa catcgccgat caataccgct gtcggatgga caaaactctc ttctggcaca
    13561 accggagtca gaccatctat ctggtaaatt ggcatatcgc ctcctgttca atattcaaac
    13621 cgccaaaacg ttgaaaatac aggcttcccg gcgcaggcag ttcaccgacc gaggtttcac
    13681 ctttttcact aacgaacgct aaagcagcag gcgctacgcg ctgataaatg ttgatacaca
    13741 gctgacgcgc cgtatgccct gcccagtgcg ccggaagcaa ctcctctggc aacaatgggt
    13801 ctttaaggac gacacggcga taaaaatgga tcagtaaaag ctgaatatga aatgcacgct
    13861 ccggggttaa ctcgtctgct gcctctttta acagcggcac cagcgggcgg aatgactgaa
    13921 taaaggtttc gtacatggca ttttgttcag ttaaatgcca gcactcttcc actctggcac
    13981 gcagtgctgc acgagaaagc gccagtggta tttgcgcttc aaaacaaatc acgttatccg
    14041 ccacccccgc ttcatgcaaa agtgtctgta catccgccag tttttgcgac ggcgatgcca
    14101 tcaggctggg tgccagtgcg ccaaaacctt gccagatcaa ctgctttttg acatcagcca
    14161 gcgttgattt atctaaacct tccgagagca acaggagcca tttaccatcc catgcaggtt
    14221 gctctgcgcg ataaatttta ctttctgccc gtcgcgtcag gcgcaagcct ttatcactga
    14281 ggctatagaa actgcgtcgc ccgatgcggg aaacatccag ccagccttct ttattaagac
    14341 gaaacaaagc ggtgcgcacg aaacgctcac caaatcccag cccttccagc aaagcagcca
    14401 gactacccag ccagatttca ccaccacgat gggaaagcga atcaccatac agagaggaga
    14461 tcaaagatgt gccactgacc ggaacagcgt ttacagcatg ttggataaaa gtaacaagtt
    14521 tactcattcg attcagtctg ttgttattgt tgtgatttat gaatcatagc acaggtcgta
    14581 tcgacttctg gccctggcgc tttacctcac cagggccaga cgacacgtca ggcaccaaca
    14641 atattgcgca gatcaaacac ccgacacgcc ttgccttctg aacgcgggat actgccacag
    14701 ttaacgatca tcacatcggt agagatcccc accatcgact taatccgatg gcgcagctgg
    14761 tggcacacct ggcaacgctg ctcatgcgtc aatgtcaggc tgctttcttt caactccact
    14821 ttcacagaaa gtgaatcaag atgcccccgg cggttcacct ccagttggta atggggcgat
    14881 aaatgttcga atttgacaat ctcttcttcc agttgtgacg ggaagacatt tacaccgcga
    14941 atgatcaaca tgtcatcgct gcgtccgctg atgcgatcca tgcggcgcat agtccgcgcc
    15001 gttcctggta acagtcgggt taaatcacgc gtgcggtaac gaatgaccgg caatgcttct
    15061 ttggtcagcg tggtgaataa cagttcgcca tgttcaccat cggcaagcgg tgtgccgtca
    15121 tgaggattaa caatttcagg atagaaatga tcttcccaaa tggttgggcc gtcggtagtt
    15181 tccagacact ccatcgccac ccccggcccc atcacttctg acaggccata aatatccagt
    15241 gcggtgatcc ccagacgccg ttcaatctct ttacgcatgg cctgtgtcca cggctcagca
    15301 ccaaacactc caacccgcag cgagcaaccg ctggcatcac cgcccaactg ccgctccagc
    15361 tcttcaatca ggttaaggca ataagatggc gtaaccatga tcatatctgg ttgaaaatcg
    15421 cggatcagtt gcgcttgttt ttccgtctgc ccaccagaca tcgggatcac cgtagcgccc
    15481 agacgttcgg caccatagtg cgcgcccagc ccaccagtaa acaggccgta accataggca
    15541 acatgaattt tgtctttggg cgtgccacct gccgcacgca aggaacgcgc tacaatattg
    15601 gcccagttat caatatcgtt ttgcgtatag ccgacaactg tcggtttacc tgtggttccc
    15661 gaagaagcat gaatgcgcac cacttgttcc atcggcacgg caaaggtgtc gaagggatag
    15721 ttgtcgcgca gatcctgttt ggtggtacag ggaaatttac gcagatctga aagttccctg
    15781 aaatcatcag gatgtacgcc tgctgcgtca aatttgcacc gatacatcgg gacattttca
    15841 tacgcgtgtt tgagcgtcca tttcagacgt tgtgtttgca acgcctgtaa ctcatcaaca
    15901 gacgcggttt caatcgggtc aagctttgta ttggttatca ttgtagggta ctcgcaggtt
    15961 gatatgctca aacacgctcc agaatcatgg cgatgccctg accgacaccg atgcacatgg
    16021 tgcacaatgc gtaacgaccg ttacgccgat gcagctcatg gctggcagcc agtgccaggc
    16081 gggcaccact cattcccaac ggatggccta aggcgatagc gcctccgttg gggttaacat
    16141 gtggggcatc atcaggcagc cccaattcgc gtagtacacc caacgcctgg gccgcgaacg
    16201 cttcgttcag ttcaatcacg tccatatcgt gaatactcag ccccgcgcgt tccagcaccc
    16261 ggcgagttgc aggcaccgga ccaagcccca tcaggcgcgg ttccaccccg gcggttgcca
    16321 tggctacgat tcgcgcccgc ggtgtcagtc cttgcgctgc tgccatctgt tcactggcaa
    16381 taatcaacgc agcggctccg tcattcaccc cggaggcatt gcctgcggta atcaccccat
    16441 tggcacgaaa tggtgctttt aacccacgta actgttccag cgtcgtttcc gggcgcagat
    16501 gctcatcatg ttgtatttct gttacaacgc ctttcttgtt tttcaacaca accggaacaa
    16561 tctcctcagc cagaatgcct gaggattgcg cttttgccgt acgttgctga ctgcgtagcg
    16621 caaaactatc ttgatcttct cgcgatattt ttaacagttc agctacattc tctgccgttt
    16681 ccggcatgct gtcagttcca aattgttgag ccatgagcgg gttcacaaat cgccagccaa
    16741 tagtggtatc aaacatctca gcctgacgag aaaatgcact ggttgccttg cccataacaa
    16801 acggtgcccg agacattgac tccacgccac cagcgatcag caaatcgcca tcgcccgctt
    16861 taatcgcccg tgcggcgaac cccagtgcgt ccagcccgga accacacaag cggttaatgg
    16921 ttgtgccgga aacactctgc ggcagccccg ccagtaacgt cgccatccgg gctacgttac
    16981 ggttgtcttc tcccgcctga ttagcacagc cgaggatcac atcatcgata cactccgcat
    17041 cgagacgcgg gtttcgcacc agcagctccc gcaaagggat agcagccaga tcgtcagccc
    17101 gaacacctga taatgcccca ccatagcggc caattggcgt acgaattccg tcacagataa
    17161 aggcttcacg catcaggctt ctcctgtaat ggtgccgccg atgcggtgag atttaccgcg
    17221 aaacagcgca accgtttttt gttgttggtt aacaatttcg atgtcgtaaa caccggtttg
    17281 cttgccctgg tgacgtacct gcgcagtagc agttaaggtg tctccggcaa agcctggacg
    17341 caaaaaatca atcgtgcagg cagaagcgac ggctgccagc ccctggctat tgcaggcgta
    17401 ggcaaaggca gtatcagcca gtgaaaatag ctgcccgccg tggcaacttt gatgaccgtt
    17461 aagcatttgt gcagtgacgg tcatggtcac tacagcaaag ccttcatcca ttgagataat
    17521 gtcgatgcca agcgctttgg cgcaggcatc gttctcatac attgcatggg cattttgcca
    17581 ggccttatga ctcataaccg ctctccagaa gcgctcgttg gcgcagcaat gaacatgggc
    17641 gatagcgttc ttcgccatag tgatgttgta gattttccag gagccttaat attcgctgcc
    17701 agccaagttg cgctccccag gcaagcgggc catatggata attcacccca agacgcatgg
    17761 cggtatcgat atcctgttca gaggccacgc ctttttgcag cgcatcaagg gcttcattga
    17821 tgatcattgc taccgttcgc caaatcagca ttcctgggta atctgcaatt tgcagcactg
    17881 ttttgccctg ctgttgcagg taataaatgg ccttgcgggt cgctgagtcc gggttcaccg
    17941 ctgccgcagc aatggtcacc accttgcccg ccattttgtc gaccactacc accgggcgtg
    18001 ccagtcgtat tgccagcgcc tgtgccgtct cgccttgtgt ctcaatcaat aaaacatcgt
    18061 caatttccgt gacgccgtca cttttctttt ctactttcat tgggctaaaa ctgtcgctta
    18121 ccgcttccag gccaacaact gcctcacgtt ccgcgcgcca gtcgtacacg cccagcccac
    18181 ttttcttgcc caaccgtcca ccaatcacca gttcctgttg caccagcgaa ggtaaaaaac
    18241 gacgctcctg ccagaaagcg ttaaacaccg aacaggtgac agcaaaattg acgtcctgac
    18301 caatcagatc ggttaattcc agcggcccca tcgggaaacc agcgccatcg cgaagtgcag
    18361 cgtcaatcac ttctggtgca gcaacctgct cttccagtgc ccgccaggcc tcggaataat
    18421 aaggacgcgc aacacggtta acgataaatc cgggggtcga atgacagcgc actggctgct
    18481 taccccaact caatgtcagt tcacacaact gctcgacaac ttccgctgcc gttgccagcc
    18541 cactgaccac ctccaccaac ttcatcaccg gtgccgggtt aaaaaaatgc agccccgcaa
    18601 cacgttcagg attttttacc tccgcagcaa tcgcggttat agagattgac gaagtgttag
    18661 tggtcaatag cgtttgtggc gggcaaactt ccgccagttg tgcaaagagc gcttttttaa
    18721 cttccagacg ttcagacgcc gcttcaataa ccagattcgc tgctgccagc gcgtgaatat
    18781 cggtcaccgg gatcaggcgt ttcaatgtgc gttcacaggt ttcagcggtc agttttcccc
    18841 gcgtcacgcg tgaatttagc cgcgcgtgta tcccgtcgat tgcgcgggtc agcgcttcag
    18901 cagaaatgtc atacagtaaa acctgatgtc catgactggc ggcaacttca gcaatgcctg
    18961 cccccatggt gccgctccca atcactgcca cagtttgcac atttatcatc atgctatttc
    19021 cccgtgaact gcggtgagcg tttagccagg aacgcactga caccttcacg ataatctgcg
    19081 ctccgtccgg caagtcgctg atagtcacgt tccagatcca gttgcgtatc gagcgtattg
    19141 gtttcagcgc tatttatcgc ttgcttgata agtccaagac caaatgtcgg ttgtgtcgcc
    19201 agatgccgtg ccagctgttg cgcggtatct gccagcgttt catcatcaac aacctgccag
    19261 atcatccccc attcgtgcgc ctgttcagca ctcagttgat tccccagcag tgccagcccc
    19321 atggcgcgcg ctcgcccagc aacgcgtggc agtaaccagg ttccaccgca atcgggtact
    19381 aaacctaact tactaaacgc catgacgaat tttgctgaac gggcagcaat aacgatgtcg
    19441 catcccagtg ccagtgttgc gccagcccct gccgccacgc cattgactgc acagatcacc
    19501 ggttttggca gttttgccag gcgacgtacc agtgggttat agaaacgttc aactgacatc
    19561 cctaaatccg gtgcggggcc tgtgggatca acgttacgat cgttaagatc ctgaccagca
    19621 caaaatccgc gcccggcacc tgtaagtaac aggcaacgga tagtgtcgtc gcgctcgacc
    19681 tgtttcaggc actctgccag ttgtgcgtgc atctcatcat taaaactgtt caggcgttcc
    19741 gggcggttga gcgttagtgt catcacgccc ttttctacat gactgaggat gaattccatc
    19801 attagcgtcc tttaaagtcg ggcgtgcgtt tttgcaagaa agcggagatg ccttcatgac
    19861 gatcttctgt tgccgccagc aaggtgaata actgtcgctc ctgggcaagt ccggcttgca
    19921 aagccacttc ctgcgactgg cgcagcgctt gctttgccgc ttgtaaggcc agcggcgagt
    19981 gacgtgccat tttcgacgcc agctgtaagg cgtattcgag ggttaaatcg ctggggaaga
    20041 cgtcgctaac cagcccggcc tgctgtgctt gctgagcggt gatactttct ccgctcagca
    20101 ccattttgct ggctaacgat ttaccggcac tacggattaa acgttgcgtt cctcctgcgc
    20161 ctggcatgat gccgagagtg atttccggca aaccaaaacg cgcgttctct ccggcaacca
    20221 ccacatcgca caacaatgcc agttcgcaac ccgcaccaag cgcgtaaccg ttgacggctg
    20281 cgatgagagg tttgttgaag gcctgcaatc gcgcccatag ctgcgggcgt gtatcgttta
    20341 aggtggccgc gagatctttt tctgccattt cgttgagatc ggccccagcg gcaaaaaagc
    20401 gtgcattacc ggtaatcaca cagaccgaaa tgctgctatc ggtagccgca gcttccagtt
    20461 catttaccag ttgcgtcaga agggcattat ttagcgcatt acgtgcggcg ggacggttaa
    20521 gggtcagcaa caatacttgt tgctgacggc tgacgatcag ttcgctcatg ccatcccctt
    20581 cgcgtcaaag tcaaccacca catcgctggt cagcggcagt gcctggcaac tcaacacata
    20641 gcctgcggcc agttcatccg gttccagact gtaattggtt tccatcgcca ctttgccacg
    20701 cagcactttg catttgcagg tcgcacagac gccgcctttg caggcatagg gcagatcagc
    20761 cccctggcgc aatgccgcat cgagaatgct ttcatcgtcg gcattaagca cgatttcccg
    20821 atcccgccca tcctgacgta cagtcacttt ttgtccgtca ctttgcacgt taacgctacg
    20881 tttgacgcgc gtgccaggcg tattaaaccg ctccagatga atggttttat ctggcattcc
    20941 cagtgctttt aaggcggctt ccgtctcatc catcatcgcc gccggaccac aaataaatgc
    21001 ctcatcataa agacgaaaat taatgagcga ggccccaagt gactgtaatt tttcaccgtc
    21061 aatacgcccg tgaagcagat cgctgtcgag ggtttcctga ctgaaaatgc acaacaactg
    21121 taaacgctga ggatatttgt ctttcaggtc tgccagtgcc tggcgaaaca tcatgctctg
    21181 gctggtacgg ttaccgtaga tcagggtgaa ctgactttca ggctcggttt gtaaagtggt
    21241 ggcgataatc gccagcattg gcgtaatacc tgatcctgct gcaatagcca gatagcggcc
    21301 ctggcgttcg gcctgtggct gatagccgaa atgcccctgc ggcaccatga cctccagcgt
    21361 cataccctgg cggatgtgtt cgcgggcata gcgggagaaa cgtccgcctt caatggcttt
    21421 caccgccaca ctaatttcgc caggcagata gctgcggcaa atggagtaac aacggcgtaa
    21481 ttcttcacca tcaaagctgg cttttaaggt caaatgttga ccggggcgaa agcgatacgc
    21541 ctcctgcaaa ggctggggca ccgcaaaggt aatggtcacc gcatcacggg tttccggctc
    21601 cacttttgcc accgttaagg aatgaaacgt tgtcatggca tcctcaaata catttgaaat
    21661 aatcgaaagg ttcgcggcaa ctatcgcagc gatacaatgc tttgcaggcc gtggaaccaa
    21721 attcactgat aagtgtggta tggacgctgg cgcagcgcgg gcaacgtact tctggcggca
    21781 aatgggcatg gcaactgtgt ccggcgggcg ggctaatgcc atactgtcgc agacgctcac
    21841 gggcatcggg ggtcatccag tcggtggtcc atgccgggtc gagttgcagc acaacctgaa
    21901 cgggggtaaa cccgtgggtt gtcattgcct cacgtatcgc gccaatcaaa tgttccgttg
    21961 ccggacaacc ggaataagtc ggcgtaaagc cgatcaccca tccttctccc atctgtgtca
    22021 cattacgcac catgcctaaa tcggtaatgg tcagcaccgg gatctccggg tccgggatct
    22081 ggctgagtag cgcccatatc tcatgaacct gtggcggtgc aatggtggcc agacgttgca
    22141 tacccatctc ctctgttacc attgctgacc gggcaagaca cactggagat actgcatttc
    22201 tgccagcatg ggtccaaggt gttcggtatg cagtcctttt ttaccgccag tgcgatacgc
    22261 ctgctcttgc ggtacgttca atgtggcttc gttgattccg gcaaaaactt cggcttccca
    22321 cgctgcgcgt aaagtgcgtg gatcaaccgc aataccctct tcactcagtg caatatcaat
    22381 ctcgtcggca tcgaacagtt cggcagtaaa acgccacaac ttgtcgatgg cctgctgcat
    22441 cttttgccct gatacgtcag taccattgcc cagtcgctcc agccagccac gactaaaacg
    22501 caggtgataa cgcgcttctt taattgcttt ggcagaaatc gccgccagtt gcggatcacg
    22561 gctttccatc agacgggtaa agagcgccac atgccatgca tcgatgaaat actggcgtgc
    22621 aatggtgtcg gcaaaattgc cgtttggttg ttcaaccaac aataagttgc tgaactggcg
    22681 ctcgtctcgg gtgaaggcca gggtatcttc atcaccttct cccgctaatt cagcggcata
    22741 cgataagaag ttacgtgcct gacctaataa atcgaggcca atgtttgcca gtgcgagatc
    22801 gatttccagt tccggtgcgt gaccgcacca ttcccccaac cgctgggaga gcaccagaca
    22861 gttatcgccc aggcgcaagg tgtaagccgt taactgattc atttccgacc tcacatgtgc
    22921 tcaatgccat cagggatggt gtaaaacgtt ggatggcgat agaccttgct ttcagccgga
    22981 tcaaaaaatt caccgcgttc ttccggttgc gaggcaacaa tttcactcgc cttcaccacc
    23041 caaattgaac atccttcgct acgacgggtg taagcatcac gggcattttc cagtgccatc
    23101 cgctcatcgg cagcatgtaa actgccaaca tggcggtgtg ataagccctg tttgccacgc
    23161 acgaaaactt cgtataacgg ccagtaaaca ttactcattt tgaatctcct tatgcgacct
    23221 tacgggcaag ttgtttttgt gcatgggcca gcgctgcttc ccgtacccag gtaccttctt
    23281 cccaggcttt acgtttggcg tcgagcctct cctgattaca aattccgcga ccgttaatta
    23341 cttcgttaaa ctcctgccag tcgatctcac caaagcggta gtgaccgctt tcagtgtcaa
    23401 aatgcagatc cgggtcagga acggtcatac cgagcatttc aacctgtgga atggtgttat
    23461 ccacgaaacg ctggcggagt tcgtcgttgg tgaaacgttt gattttccag gcgagacttc
    23521 tggcgctgtt tggcgagtta tcatcgtttg gcccgaacat cattaaggct ggccaccaga
    23581 aacggttaat ggcgtcttgc aacatctgct tttgagcttc gctgccctgc gcgagtgcca
    23641 tgcaggcttc aaacccctga cgctggtgaa aactctcttc tttgcagatt ttcaccattg
    23701 ctctggcata cgggccataa gaagtgcgac ataacgccac ctgattgacg atcgctgcgc
    23761 catccaccag ccagccgata acgccgatat cagcccaact caatgtcgga taattaaaga
    23821 tggaggaata tttcatccgt ccgtcgagca ttttttggta gatgtcttct ctggcgcagc
    23881 ccagtgtttc ggcggcgcta tagagataca gaccatgacc ggcttcatcc tgcactttgg
    23941 ccaacagaat ggctttacgc cgcaaggttg gtgcgcgagt gatccagtta ccttcaggca
    24001 acataccaac aatctcggag tgcgcatgct gcccaatctg gcggatcagt gtctttcggt
    24061 aagcatcggg catccagtcc tgtggctcga tagccgtttc ctgggctatc cgttgctcaa
    24121 agcgttcttc ttgggtcacg tcatcacctt tacgattcca gttttcgtga attaattaaa
    24181 aacacgaatc acattgtttt gtgaaagtta cacaatggtt aactaaaatc acaaattttg
    24241 tatgcttgaa ctgtgacgga tttcgcaccc tatttttata aatcattatt tatcagtgca
    24301 ttaatttttt ccgcagttgc tatacagatc gcatagttaa catttcgtta aaagaaccct
    24361 tgctttttat gattcgcgat ttaactatta gcaacagaaa tgtgaaacat ctggagagta
    24421 gcgatgcagc agttagccag tttcttatcc ggtacctggc agtctggccg gggccgtagc
    24481 cgtttgattc accacgctat tagcggcgag gcgttatggg aagtgaccag tgaaggtctt
    24541 gatatggcgg ctgcccgcca gtttgccatt gaaaaaggtg cccccgccct tcgcgctatg
    24601 acctttatcg aacgtgcggc gatgcttaaa gcggtcgcta aacatctgct gagtgaaaaa
    24661 gagcgcttct atgctctttc tgcgcaaaca ggcgcaacgc gggcagacag ttgggttgat
    24721 attgaaggtg gcattgggac gttatttact tacgccagcc tcggtagccg ggagctgcct
    24781 gacgatacgc tgtggccgga agatgaattg atccccttat cgaaagaagg tggatttgcc
    24841 gcgcgccatg tactgacctc aaagtcaggc gtggcagtgc atattaacgc ctttaacttc
    24901 ccctgctggg gaatgctgga aaagctggca ccaacgtggc tgggcggaat gccagccatc
    24961 atcaaaccag ctaccgcgac ggcccaactg actcaggcga tggtgaaatc aattgtcgat
    25021 agtggtcttg ttcccgaagg cgcaattagt ctgatctgcg gtagtgcggg cgaccttttg
    25081 gatcatctgg acagccagga tgtggtgact ttcacggggt cagcggcgac cggacagatg
    25141 ctgcgagttc agccaaatat cgtcgccaaa tctatcccct tcactatgga agctgattcc
    25201 ctgaactgct gcgtactggg cgaagatgtc accccggatc aaccggagtt tgcgctgttt
    25261 attcgtgaag ttgtgcgtga gatgaccaca aaagccgggc aaaaatgtac ggcaatccgg
    25321 cggattattg tgccgcaggc attggttaat gctgtcagtg atgctctggt tgcgcgatta
    25381 cagaaagtcg tggtcggtga tcctgctcag gaaggcgtga aaatgggcgc actggtaaat
    25441 gctgagcagc gtgccgatgt gcaggaaaaa gtgaacatat tgctggctgc aggatgcgag
    25501 attcgcctcg gtggtcaggc ggatttatct gctgcgggtg ccttcttccc gccaacctta
    25561 ttgtactgtc cgcagccgga tgaaacaccg gcggtacatg caacagaagc ctttggccct
    25621 gtcgcaacgc tgatgccagc acaaaaccag cgacatgctc tgcaactggc ttgtgcaggc
    25681 ggcggtagcc ttgcgggaac gctggtgacg gctgatccgc aaattgcgcg tcagtttatt
    25741 accgacgcgg cacgtacgca tgggcgaatt cagatcctca atgaggaatc ggcaaaagaa
    25801 tccaccgggc atggctcccc actgccacaa ctgttacatg gtgggcctgg tcgcgcagga
    25861 ggcggtgaag aattaggtgg tttacgagcg gtgaaacatt acatgcagcg aaccgctgtt
    25921 cagggtagtc cgacgatgct tgccgctatc agtaaacagt gggtgcgcgg tgcgaaagtc
    25981 gaagaagatc gtattcatcc gttccgcaaa tattttgagg agctgcaacc aggcgacagc
    26041 ctgttgactc cccgccgcac aatgacagag gccgatattg ttaactttgc ttgcctcagc
    26101 ggcgatcatt tctatgcgca tatggataag attgctgctg ccgaatctat tttcggtgag
    26161 cgtgtggtgc atgggtattt tgtgctttct gcggctgcgg gtctgtttgt cgatgccggt
    26221 gtcggtccgg tcattgctaa ttatgggctg gaaaacttgc gctttatcga acccgtaaag
    26281 ccaggcgata ccatccaggt gcgtctcacc tgtaagcgca agacgctgaa aaaacagcgt
    26341 agcgcagaag aaaaaccaac aggtgtggtg gaatgggcgg tagaggtatt caatcagcat
    26401 caaaccccgg tggcgctgta ttcaattctg acgctggtgg ccaggcagca cggtgatttt
    26461 gtcgattaat cggtgaatga agggcaacgg cgaatagttg cccttttatt tcaccaaatt
    26521 ttgtgacgtt gtcacgttat gcatgatgtg tatctgtatt ttcagggcac ccactgtatg
    26581 aaaagctggc acacctgcca aacaacctgg caggtgcagg caatcccctt tacatcagta
    26641 ctgataatgt gaacctgact aaaccgccca cagagcgcgg ttgctaacaa gaacacaaca
    26701 tctgacgagg ttaataatgg gaagcccctc tctgtattct gcccgtaaaa cagccctggc
    26761 gttggcagtc gccttaagtt tcgcctggca agcgccggta tttgcccacg gtggtgaagc
    26821 gcatatggtg ccaatggata aaacgcttaa agaatttggt gccgatgtgc agtgggacga
    26881 ctacgcccag atctttaccc tgattaaaga tggcgcgtac gtgaaagtga agcctggtgc
    26941 gcaaacagca attgttaatg gtcagtctct ggcactgcaa gtaccggtag tgatgaaaga
    27001 caataaagcc tgggtttctg acacctttat taacgatgtt ttccagtccg ggctggatca
    27061 aacctttcag gtagaaaagc gccctcaccc acttaatgcg ctaactgcgg acgaaattaa
    27121 acaggccgtt gaaattgtta aagcttccgc ggacttcaaa cccaataccc gttttactga
    27181 gatctccctg ctaccgccag ataaagaagc tgtctgggcg tttgcgctgg aaaacaaacc
    27241 ggttgaccag ccacgcaaag ccgacgtcat tatgctcgac ggcaaacata tcatcgaagc
    27301 ggtggtggat ctgcaaaaca acaaactgct ctcctggcaa cccattaaag acgcccacgg
    27361 tatggtgttg ctggatgatt tcgccagtgt gcagaacatt attaacaaca gtgaagaatt
    27421 tgccgctgcc gtgaagaagc gcggtattac tgatgcgaag aaagtgatta ccacgccgct
    27481 gaccgtaggt tatttcgatg gtaaagatgg cctgaaacaa gatgcccggt tgctcaaagt
    27541 catcagctat cttgatgtcg gtgatggcaa ctactgggca catcccatcg aaaacctggt
    27601 ggcggtcgtt gatttagaac agaaaaaaat cgttaagatt gaagaaggtg cggtagttcc
    27661 ggtgccaatg accgcacgcc catttgatgg ccgtgaccgc gttgctccgg cagttaagcc
    27721 tatgcaaatc attgagcctg aaggtaaaaa ttacaccatt actggcgata tgattcactg
    27781 gcggaactgg gattttcacc tcagcatgaa ctcgcgcgtc ggacctatga tctccaccgt
    27841 gacttataac gacaatggca ccaaacgcaa agtcatgtac gaaggttctc tcggcggcat
    27901 gattgtgcct tacggtgatc ctgatattgg ctggtacttt aaagcgtatc tggactctgg
    27961 tgactacggt atgggcacgc taacctcgcc aattgcacgt ggcaaagatg caccctctaa
    28021 cgcagtgctc ctgaatgaaa ccatcgccga ctacactggc gtaccgatgg agatccctcg
    28081 tgctatcgcg gtatttgaac gttatgccgg gccggagtat aagcatcagg aaatgggcca
    28141 gcccaacgtc agtaccgaac gccgggagtt ggtggtgcgc tggatcagta cagtgggtaa
    28201 ctatgactac atttttgact ggatcttcca tgaaaacggc actattggca ttgatgccgg
    28261 tgctacgggc atcgaagcgg tgaaaggtgt taaagcgaaa accatgcacg atgagacggc
    28321 gaaagatgac acgcgctacg gcacgcttat cgatcacaat atcgtgggta ctacacacca
    28381 acatatttat aatttccgcc tcgatctgga tgtagatggc gagaataaca gcctggtggc
    28441 gatggacccg gtggtaaaac cgaatactgc cggtggccca cgcaccagta ccatgcaagt
    28501 taatcagtac aacatcggca atgaacagga tgccgcgcag aagtttgatc cgggcacgat
    28561 tcgtctgttg agtaacccga acaaagagaa ccgcatgggc aatccagttt cctatcaaat
    28621 tattccttat gcaggtggta ctcacccggt agcaaaaggt gcccagttcg cgccggacga
    28681 gtggatctat catcgtttaa gctttatgga caagcagctt tgggtaacgc gttatcatcc
    28741 tggcgagcgt ttcccggaag gcaaatatcc gaaccgttct actcatgaca ccggtcttgg
    28801 acaatacagt aaagataacg agtcgctgga caacaccgac gccgttgtct ggatgaccac
    28861 cggcaccaca catgtggccc gcgccgaaga gtggccgatt atgccgaccg aatgggtaca
    28921 tactctgctt aaaccatgga acttctttga cgaaacgcca acgctagggg cgctgaagaa
    28981 agataagtga ttgtttcaga caaaaaaacg gcaccaggtg ccgtttttta cttatgagcg
    29041 aaccagatta ataccgtaca cacaccgact tagtttcaca ccaaccgtcc agccagtcgg
    29101 ggccaaaatc acggcccgtt cctgactgct tcatcccacc aaacggtaag ttagcgtcaa
    29161 ttaaggtatg gctgtttacc cacaccgtcc ctgcctgtaa gcgatcgcta tattccagag
    29221 cctgggagag attttgcgtc cagacactgg cagttaagcc atattccgtg tcgtttgcca
    29281 gttgtaacgc ctcttctcca tccgctactc gcaccaggtt taccaccgga ccaaacacct
    29341 cttcacgagt taagcgcaat ttagcatcgg gatttaccac cagcgttggc gcaacataat
    29401 acccctctcc ggctggtcca ttcgacccgc gaatcagctc tgcttgctgt gcctgcgcat
    29461 cgtcgaggaa tgaacacact ttgtcgcagt gcgcacgaga aaccaaaggg ttaatctgtg
    29521 caacaggtga catccccggt cccacttgca acgattttac cgcctgctca aatccactaa
    29581 ccagcgtgtc aaacaacggc gcttcaatat aaattcgcga actggcggcg catacttgcc
    29641 cttgattcag gaagcttccg gtcatcaagc cttcaataac ccattgcgga tcagcatctt
    29701 ttaatacaat tgccgggttt ttaccgccca gttccagcgt tacacgcgtt aagtgatcag
    29761 cagcagttct ggcaatacct tttcccgtcg cggttgaacc ggtaaaactg attttcgcaa
    29821 catgaggatg tgacgtcagg gccgcgccgc atacagcacc tgacccggtg acgacattaa
    29881 aaacgccatc agggatacca gcctcgctgg ccagttccgc cacgcgcaac atcgtcagtg
    29941 gcgtggtttc cgaaggctta atcacgattg aacagcctgc tgccagtgct ggcatcacct
    30001 tccacatacc aatcatcaac ggaaagttcc atggcacaat tcccgccact acgccaaccg
    30061 gctctttacg cgtccaggcc tgataacgcg ccccctgggg taagggaatc gacaagtcca
    30121 gcgttttacc cgcgattttg gtcgttaacc cggcggtata acgcatccag ttcagcgtac
    30181 agcccacttc aaaagcacgg gaaatggcaa ttgacttgcc ttgctccagg gtttccagtt
    30241 gcgccagctc ctcactgtgc tgttccacca gatcggcaaa acgtagcaga atacgttcac
    30301 gctctgcggg taatcgcccg gcccagcgac gcgagacaaa ggcccgccag gcagacatga
    30361 ctgcgttatc tacatccgct tcgttggcat cagcagtaga cgcaatttct tgcccggtgg
    30421 ccggatcaaa gatcgccaat cgtttttcac tttgtgcggg gcctggacga ccatcaatat
    30481 aaagaccgtg ttgacgatcg agaaactgtt ggacctggct taatactgct acatgcggct
    30541 ctgtcatcac ttttccttat tatttaccca gtgtgatttc agtgtacgca gcgacaagaa
    30601 aagtcacttt tttcgctgtg acattcgctt tgtcagctgt gacaagctcc gcaaatcgtg
    30661 acaataaccg cttatgtctc tatgcctgag aagacttatt tcctatagtc aggctcatta
    30721 gcttcgttta ctgcaacaca aatgcaacaa taaaaataca tttcacagag cgaaaacgtg
    30781 ccatgaaccc cgcagtggat aatgagtttc agcaatggct ttcccaaatc aatcaggtat
    30841 gcggaaattt taccggacgc ctgctgactg agcgttacac gggtgtactg gacacgcatt
    30901 ttgccaaagg actaaagctg agtaccgtga caaccagcgg ggtgaattta tcccgcacct
    30961 ggcaggaagt aaaaggcagc gacgatgcct ggttttacac cgtttttcag cttagtggtc
    31021 aggcaataat ggagcaggat gagcgtcagg tgcagattgg cgctggcgat attacgttac
    31081 tcgatgcctc acgcccctgt tcgctttact ggcaggagtc ttctaaacag atttcattac
    31141 ttttgccacg cactctgctg gaacaatatt ttccccatca aaaacctgtc tgcgcagaaa
    31201 gactggacgc tgacttaccc atggtgcaac tcagtcatcg cctgttacag gagagcatga
    31261 ataatccggc actttctgaa acagaaagtg aagctgcgct acaggcgatg gtgtgtctgc
    31321 tgcgcccggt acttcatcag cgggaatctg ttcaacctcg tcgtgaacgt cagtttcaaa
    31381 aagtggttac gttgatagac gataatattc gcgaagagat attacgcccg gagtggatag
    31441 ccggagagac aggtatgtca gtacgtagtt tgtaccgaat gtttgccgat aaaggtttgg
    31501 tagtcgcgca atatattcgt aaccgtcgtc tcgatttttg tgcagatgcg attcgccatg
    31561 ccgcagatga tgaaaaactg gcaggcatcg gctttcattg gggattttct gaccagagtc
    31621 atttttcaac ggtatttaag caacgctttg ggatgacgcc aggcgagtat cgacgtaaat
    31681 tccgctaact aattggcgtt gtgtgtactg caacgccaat tagttaaaac agatatttga
    31741 ctatgcattt ttcagggcga aaggcaattt gacaggagtt tttgaatatt caccttgttt
    31801 caggaaaata gatcaatcgc tttctcctgt agcgttatcc cgatattgcg gttttgctat
    31861 taaaattttc gcacccattt accgtttatc ccttgtacat attcccccgg tttggcccga
    31921 gctacgagct tttgcccggc gagtttcgcg atatcatcta cagacacatt attttgcttt
    31981 gccagttgtt ggtaactcgc tttacgttcg gcattaatgt cggttactaa tttctctgtt
    32041 tcagcatccg ttttcagcgc aaccagataa ccataaaatg tttcacctac ccgcccctga
    32101 gttctggctt catccagagt caatgccatt acattgctgc ttaccagccc aacaagaaac
    32161 gcacaaagaa gtaatgtttt cttcatcatc gcctcagaaa agatcgctac gagtttcaag
    32221 cagctcttcg acatctttgt ctgccttgat gatgatctca tgctcaattt taacgttcat
    32281 attgatagtg atcggttcct tgggcgcagc gacttcaata cgcggcgtac aggcagccag
    32341 cataaaagat gacgtcaacg cagccagtaa aattttcatt gtttttcctc acactctttt
    32401 ccttgcggac agtcatttcc cggcagacgt gcgttctgct ccaaccatgc ctggagatta
    32461 tcgccaaagc gtaaactgcg ccacagcgta aaaatattct cttcatgata gtaattaagg
    32521 tttaccgtac cgcttttacc atcaacgcga ctggtacctg taatgttggc ctgcatggtt
    32581 aataaaccga gattatctaa gttaattttt gtcgatgacc ggctaatttc catatagcgc
    32641 aaccagttaa ttgctgaacc cgcagtcata ttgtctttca ccaccgcatc tgcggtgtct
    32701 ttatccagcc gcaaggtcat tggcccgcta tttgccagcc aaccatcttt cactatccat
    32761 ttttcgttgt ttagccataa cggcaatgca ccactaaatg ccccggacat ggcgaattgt
    32821 ttcggattga cggcgctaac cagttcgctg gatgagagat tattcagccg caacagcgcc
    32881 gggtcatgtt gcggcatacg taattgtttc atcagtacgt taccgcctaa cacatcgacg
    32941 ctaacatcag tcaacagcaa gggttcttct tcagtccacg gataacgccc ttgcaaatca
    33001 gccgtaatat ttttcgctgt caccagatta atcacttcgg caattcgcaa cgtaacgggg
    33061 ccgcgagtcc ccagatgcca ggctccatcg gcaaaacgga aaggcaggac aaaatcgaca
    33121 ccgttaacct gattatctgg catccaggca ctaccgcctt tcaacacgcc gtgtcctccc
    33181 gcgcggaatc cttgttcagg cgcagcagaa aatgcaacct gagcatacag ttcaccatcg
    33241 cgtaagttca tcttccagtc gggtggcacc agcggctgga atacagtcag tgattgtttt
    33301 ggccaccagg cgttaccgcg cagacgaata ccgtcccagc gaccatttac ccgaaccggg
    33361 ccaatctcac cagcatgtaa atcgccttta aagaggaaat acgtcggatc gcgcccatcg
    33421 acgctaaatt ttaaggttga tggcggtaac acactgccgc cagtgaacag cgtttgcccg
    33481 gcgtccagtg acagcgcgcc gctgaaggag ggatgctgtg cgtcacgtac ccagacgacg
    33541 ggcttgtcga gaattaatcg cggcttttct accgtcatcg taccgtattg taactggtcg
    33601 aaaccggtgg agaggtcggt cagcgtaatc gtgctgtcat gccattcacc ttttcctgcg
    33661 acatcccagg tggcattcat cggtgtaaaa ctgccttttc cccagtagcg ccactgccag
    33721 cggcccgcgt cagggagaaa atcattcgcc agcccatcca tatgcagcac gaaatcgccc
    33781 agttcatttt catgcgcctg caaaatggcc tgcaaacgtc cgtcaacacc acgttgggtg
    33841 acttttacac ccgctaaagg ccagcggatt tcatcgatgt ccagcgaatc gatgactctt
    33901 ccctttgaac gaagtaacgc gcctggctca aaggtcagcg ttgggtcaga cagacttcca
    33961 cttagctgcg caggtaaacg ggcatataaa atgaggtccg cctgtttcgc ttcaccggtc
    34021 agctgcagag gcagctgact gttatccatg cttaattttc ctgggccaaa atttagcacc
    34081 gcgttgcctt tacccgcttg cccctgggtc agcacactca gtcgtccgct gatcagagcg
    34141 ttctcaagcc ctgcctgcca gttgtcgact ttgacaccca gtcggccact caaaggaaaa
    34201 cctgcatacg gccaactcca gcgaccatcg cttacggtca attgttgacg agtaatttgc
    34261 cacggcaaat cgagcaacgg atcgccgtta tcccgtgcca gcacaatcaa ttgcccgcta
    34321 ttttcctgcc agtccagctc tgcatccacc agtgacggtt cctgcggcaa gtttaacgtc
    34381 gcagtagcat gcccacttac aggaagtcca tccggcacga gcggcatagt aaattccccc
    34441 accagtttaa ccggcggctg attttcaaac gcgacgacat ccagttcgct gactgtaagt
    34501 tgttgccctt tcagctggcc ttgaaattta actttttcgc cctgataacg cagttgctgg
    34561 atatcagagg ttaatgcgag agagagtttt ccctgccatt cctgccaggg agaaaaaatc
    34621 agtttatcga tattgatcca ggtgttaggc agcatggact gccactgcgc gagggttttt
    34681 ggagcagctg gcgattgctc cgtctgcggc aatttcgcca gacaagcaga atcaagttct
    34741 accgtgccga cgttcaataa ccagcggctg ggatgtgaaa ggctggcgtt ggtgatatgc
    34801 gcaagctgac aatctcccac cagataacgg agatcgggaa tgattaaacc tttacgcgta
    34861 atgcgtgggc tttcatctaa tgcaatacgt gtcccgagcg gtagccagat gcccgccagc
    34921 gtgggaaccc acagcccgag cgtcatcagc agcgtcaacg gcacaagaat aatcagtaat
    34981 aacagcgcga gaacggcttt atatttaccc agcatgggta gttaatatcc tgatttagcg
    35041 aaaaattaag cattcaatac gggtattgtg gcatgtttaa ccgttcagtt gaaggttgcg
    35101 cctacactaa gcatagttgt tgatg
//
LOCUS       DAZFQD010000037        34141 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.037, whole genome shotgun sequence.
ACCESSION   DAZFQD010000037 DAZFQD010000000
VERSION     DAZFQD010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34141
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.037"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(87..1217)
                     /gene="dnaJ"
                     /locus_tag="ACHKTW_001891"
     CDS             complement(87..1217)
                     /gene="dnaJ"
                     /locus_tag="ACHKTW_001891"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFO6462923.1"
                     /translation="MAKQDYYEILGVSKTAEEREIKKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(1306..3222)
                     /gene="dnaK"
                     /locus_tag="ACHKTW_001892"
     CDS             complement(1306..3222)
                     /gene="dnaK"
                     /locus_tag="ACHKTW_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFO6462924.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTTPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            3599..4003
                     /gene="yaaI"
                     /locus_tag="ACHKTW_001893"
     CDS             3599..4003
                     /gene="yaaI"
                     /locus_tag="ACHKTW_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HFO6462925.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKILGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            4029..4742
                     /gene="msyB"
                     /locus_tag="ACHKTW_001894"
     CDS             4029..4742
                     /gene="msyB"
                     /locus_tag="ACHKTW_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HFO6462926.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSVLM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKNMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSAYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            4891..5457
                     /gene="satP"
                     /locus_tag="ACHKTW_001895"
     CDS             4891..5457
                     /gene="satP"
                     /locus_tag="ACHKTW_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HFO6462927.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(5492..6079)
                     /gene="mog"
                     /locus_tag="ACHKTW_001896"
     CDS             complement(5492..6079)
                     /gene="mog"
                     /locus_tag="ACHKTW_001896"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HFO6462928.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAAGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(6194..7147)
                     /gene="tal"
                     /locus_tag="ACHKTW_001897"
     CDS             complement(6194..7147)
                     /gene="tal"
                     /locus_tag="ACHKTW_001897"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFO6462929.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPTLLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            7426..8856
                     /gene="yaaJ"
                     /locus_tag="ACHKTW_001898"
     CDS             7426..8856
                     /gene="yaaJ"
                     /locus_tag="ACHKTW_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HFO6462930.1"
                     /translation="MPDFFSFINSVLWGSVMIYLLFGAGCWFTFRTGFVQFRYIRQFG
                     KSLKNSIHPQPGGLTSFQSLCTSLAARVGSGNLAGVALAITAGGPGAVFWMWVAAFIG
                     MATSFAECSLAQLYKERDVNGQFRGGPAWYMARGLGMRWMGVLFAVFLLIAYGIIFSG
                     VQANAVARALSFSFDFPPLVTGIILAVFALLAITRGLHGVARLMQGFVPLMAIIWVLT
                     SMVICVINIGQLPHVIWSIFESAFGWQEAAGGAAGYTLSQAITNGFQRSMFSNEAGMG
                     STPNAAAAAASWPPHPAAQGIVQMIGIFIDTLVICTASAMLILLAGNDTTYMPLEGIQ
                     LIQKAMRVLMGSWGAEFVTLVVILFAFSSIVANYIYAENNLFFLRLNNPKAIWCLRIC
                     TFATVIGGTLLSLPLMWQLADIIMACMAITNLTAILLLSPVVHTIASDYLRQRKLGVR
                     PVFDPLRYPDIGRQLSPDAWDDVSQE"
     gene            8926..9702
                     /gene="yaaA"
                     /locus_tag="ACHKTW_001899"
     CDS             8926..9702
                     /gene="yaaA"
                     /locus_tag="ACHKTW_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HFO6462931.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQQLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPENARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEASSSNGELVFKRYEQR"
     gene            complement(9855..10151)
                     /gene="yaaX"
                     /locus_tag="ACHKTW_001900"
     CDS             complement(9855..10151)
                     /gene="yaaX"
                     /locus_tag="ACHKTW_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HFO6462932.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAPHDHHGGHGPGKHHR"
     gene            complement(10365..11651)
                     /gene="thrC"
                     /locus_tag="ACHKTW_001901"
     CDS             complement(10365..11651)
                     /gene="thrC"
                     /locus_tag="ACHKTW_001901"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HFO6462933.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIAGDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSLGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFAALRKLMMNHQ"
     gene            complement(11652..12584)
                     /gene="thrB"
                     /locus_tag="ACHKTW_001902"
     CDS             complement(11652..12584)
                     /gene="thrB"
                     /locus_tag="ACHKTW_001902"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HFO6462934.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVTPVDGALLGDVVTVEAAETF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(12586..15048)
                     /gene="thrA"
                     /locus_tag="ACHKTW_001903"
     CDS             complement(12586..15048)
                     /gene="thrA"
                     /locus_tag="ACHKTW_001903"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009067 - aspartate family amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HFO6462935.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTNVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHLLRHAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELMKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGACRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(15074..15193)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(15129..15194)
                     /gene="thrL"
                     /locus_tag="ACHKTW_001904"
     CDS             complement(15129..15194)
                     /gene="thrL"
                     /locus_tag="ACHKTW_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HFO6462936.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(15408..16094)
                     /gene="yjtD"
                     /locus_tag="ACHKTW_001905"
     CDS             complement(15408..16094)
                     /gene="yjtD"
                     /locus_tag="ACHKTW_001905"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HFO6462937.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDIIDNIKVFPTLAESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPAKSDTTADQHQLQALRERVMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            complement(16494..16634)
                     /gene="yjjY"
                     /locus_tag="ACHKTW_001906"
     CDS             complement(16494..16634)
                     /gene="yjjY"
                     /locus_tag="ACHKTW_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjjY"
                     /protein_id="HFO6462938.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            16730..17446
                     /gene="arcA"
                     /locus_tag="ACHKTW_001907"
     CDS             16730..17446
                     /gene="arcA"
                     /locus_tag="ACHKTW_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HFO6462939.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(17505..18857)
                     /gene="creD"
                     /locus_tag="ACHKTW_001908"
     CDS             complement(17505..18857)
                     /gene="creD"
                     /locus_tag="ACHKTW_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HFO6462940.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTAQEDDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELDEPNITLGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLKLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREVSESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQCQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHTGFTGAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTTDDELRIWK
                     "
     gene            complement(18915..20339)
                     /gene="creC"
                     /locus_tag="ACHKTW_001909"
     CDS             complement(18915..20339)
                     /gene="creC"
                     /locus_tag="ACHKTW_001909"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HFO6462941.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDSQG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMDGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPELGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTVVDVAALF
                     RRVSEARTVQLAEKNITLHVMPTEVNVAAEPALLEQALGNLLDNAIDFTPESGRITLS
                     AEVDQEHVALKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(20339..21028)
                     /gene="creB"
                     /locus_tag="ACHKTW_001910"
     CDS             complement(20339..21028)
                     /gene="creB"
                     /locus_tag="ACHKTW_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HFO6462942.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSSPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDTHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(21041..21514)
                     /gene="creA"
                     /locus_tag="ACHKTW_001911"
     CDS             complement(21041..21514)
                     /gene="creA"
                     /locus_tag="ACHKTW_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HFO6462943.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSYRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            21725..22594
                     /gene="robA"
                     /locus_tag="ACHKTW_001912"
     CDS             21725..22594
                     /gene="robA"
                     /locus_tag="ACHKTW_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HFO6462944.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(22591..23238)
                     /gene="gpmB"
                     /locus_tag="ACHKTW_001913"
     CDS             complement(22591..23238)
                     /gene="gpmB"
                     /locus_tag="ACHKTW_001913"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HFO6462945.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            23290..23811
                     /gene="yjjX"
                     /locus_tag="ACHKTW_001914"
     CDS             23290..23811
                     /gene="yjjX"
                     /locus_tag="ACHKTW_001914"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
                     /protein_id="HFO6462946.1"
                     /translation="MHQVVCATTNPAKIQAILQAFHEIFGEGSCHIASVAVESGVPEQ
                     PFGSEETRAGARNRVANARRLLPEADFWVAIEAGIDGDSTFSWVVIENTSQRGEARSA
                     TLPLPAVILQKVREGEALGPVMSRYTGIDEIGRKEGAIGVFTAGKLTRASVYHQAVIL
                     ALSPFHNAVYQAL"
     gene            complement(23896..24222)
                     /gene="trpR"
                     /locus_tag="ACHKTW_001915"
     CDS             complement(23896..24222)
                     /gene="trpR"
                     /locus_tag="ACHKTW_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HFO6462947.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(24312..26249)
                     /gene="sltY"
                     /locus_tag="ACHKTW_001916"
     CDS             complement(24312..26249)
                     /gene="sltY"
                     /locus_tag="ACHKTW_001916"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HFO6462948.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLMQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSALTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTAAHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            26460..28127
                     /gene="ettA"
                     /locus_tag="ACHKTW_001917"
     CDS             26460..28127
                     /gene="ettA"
                     /locus_tag="ACHKTW_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HFO6462949.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVE
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(28434..29666)
                     /gene="nadR"
                     /locus_tag="ACHKTW_001918"
     CDS             complement(28434..29666)
                     /gene="nadR"
                     /locus_tag="ACHKTW_001918"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0050262 - ribosylnicotinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HFO6462950.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIDTVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQR"
     gene            complement(29687..31069)
                     /gene="radA"
                     /locus_tag="ACHKTW_001919"
     CDS             complement(29687..31069)
                     /gene="radA"
                     /locus_tag="ACHKTW_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFO6462951.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSMMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(31118..32086)
                     /gene="serB"
                     /locus_tag="ACHKTW_001920"
     CDS             complement(31118..32086)
                     /gene="serB"
                     /locus_tag="ACHKTW_001920"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HFO6462952.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQK"
     gene            32192..32836
                     /gene="ytjB"
                     /locus_tag="ACHKTW_001921"
     CDS             32192..32836
                     /gene="ytjB"
                     /locus_tag="ACHKTW_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HFO6462953.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            32864..33880
                     /gene="lplA"
                     /locus_tag="ACHKTW_001922"
     CDS             32864..33880
                     /gene="lplA"
                     /locus_tag="ACHKTW_001922"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
                     /protein_id="HFO6462954.1"
                     /translation="MSTLRLLISDSYDPWFNLAVEECIFRQMPATQRVLFLWRNADTV
                     VIGRAQNPWKECNTRRMEEDNVRLARRSSGGGAVFHDLGNTCFTFMAGKPEYDKTIST
                     SIVLNALNALGVSAEASGRNDLVVKTAEGDRKVSGSAYRETKDRGFHHGTLLLNADLS
                     RLANYLNPDKKKLAAKGITSVRSRVTNLTELLPGITHEQVCEAITEAFFAHYGERVEA
                     EIISPDKTPDLPNFAETFARQSSWEWNFGQAPAFSHLLDERFTWGGVELHFDVEKGHI
                     TRAQVFTDSLNPAPLEALAGRLQGCLYRADMLQQECEALLVDFPEQEKELRELSTWIA
                     GAVR"
     gene            33912..>34141
                     /locus_tag="ACHKTW_001923"
     CDS             33912..>34141
                     /locus_tag="ACHKTW_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO6462955.1"
                     /translation="MIDLENQEREIINLMFSQRISWLAAVRIRHKLSLAEVSKMLGIS
                     INSLKQIEKTERLSSNIKSKMAEIYGCPPELL"
BASE COUNT         8044 a         9304 c         8497 g         8296 t
ORIGIN      
        1 gatccccgcc actttacagg tgctcgcata tcttcaacgc accctatttt tacccaggcc
       61 tgcccacggg caggcttttg gggaggttag cgggtcaggt cgtcaaaaaa cttcttcaca
      121 ccatcaaaga agctctttga gcgcgggctg ttgtgctcgc cggttgggcc accgaagctt
      181 tcttgcagct cttgcagcag ctgtttctgc ttctcgttca ggcctaccgg tgtttcgacg
      241 acaacgcggc acagtaaatc accctgtgcg ccaccgcgga cagacttgac gcctttaccg
      301 cgcatacgga acagcttacc agtctgggtt tcgccaggca ctttcagttt gacgcgacca
      361 tcaagggtcg gtacttcgat ttcgccaccc agcgccgcca tagcgaagtt gatcgggact
      421 tcgcaataca ggttgttgcc ttcacgctcg aaaatcgggt gctgtttaac ctgaacctga
      481 acgtacagat cgcctgccgg tgcgccatgc tcgcccgctt caccttcgcc cgcaagacgg
      541 atgcggtctc cagtgtccac ccctgccggg attttaacgg acagcgtttt gctgcgctca
      601 acacgaccat gaccatgaca tttgttgcac ggatctttga tcagcgtacc gcgaccctga
      661 cagtgtggac aggtctgctg cacggcaaag aaaccctgac gcatctgcac ctgaccagaa
      721 ccatgacagg tcggacaggt ctgcggctgt gtacctggtt ttgcaccgct accgtggcaa
      781 acgtcacact cttccagagt cggaatgcgg atctctttgg tcacgccacg tacagcttct
      841 tcgagggtga gctccatgtt atagcgtaaa tcagcaccgc gcgccgcacg ttgacgacca
      901 cgtccgccgc caaaaatatc gccgaaaacg tcaccaaaaa tatcgctgaa gtctgcgccg
      961 ccgccaaaac cgccgccgcc cataccacct tgctcaaacg cagcatgacc atactgatcg
     1021 tatgccgcac gtttttgcga gtcggtcaga acttcataag cttccttgat ctctttaaat
     1081 ttcgcctcgg cctctttgtc accctggtta cggtccgggt ggtatttcat ggccaggcgt
     1141 ttgtaggcct ttttgatttc acgctcttcc gctgttttgg aaacgcctaa aatctcgtaa
     1201 taatcttgct tagccatctt ttttaaattg cccctagatg aatgcacggg cggagaggaa
     1261 attccccttc gcccgtgtca gtataattac ccgtttatag ggcgattatt ttttgtcttt
     1321 gacttcttca aattcagcgt cgacaacatc gtcatctttc gcgttgtttg cagaagcatc
     1381 agcaccggca gtctgctgct gggcatgttg ctgctgggcg atttccatca gtttctggga
     1441 aacctgtgcc agctcctgca ttttcgcttc gatagcggct ttgtcttcac ctttcagagc
     1501 agtttccagt gcagtcagtg cagactcgat agcagttttg tcgtcagccg gcagtttgtc
     1561 gcctgcttct tcaacctgct tacgggtgct gtgcagcaga tggtcgccct ggttgcgagt
     1621 ctgtaccagc tcttcaaact tacggtcagc ttcggcgtta gcttctgcgt cgcgtaccat
     1681 tttctggatt tcatcttcgt tcagaccaga agaagccttg atggtgatct tctgctcttt
     1741 accgctgttt ttatctttcg cggaaacgtg caggataccg tcagcatcga tatcgaaggt
     1801 aacttcgatc tgcggcatgc cgcgcggtgc cgggttgata ccatccaggt tgaactgacc
     1861 cagagatttg ttatcagccg cacgtttacg ttcaccctgc agcacatgga tggttaccgc
     1921 agactggttg tcttcagcgg tagagaacac ctggctgtgc ttggtcggga tagtggtgtt
     1981 tttcgcgatc agcgtcgtca tcacaccgcc catggtttcg atacccagag acagcggggt
     2041 aacgtccagc agcagtacgt ctttcacgtc accagtcaga acaccaccct gaacagcagc
     2101 accgattgct acagcttcgt ccgggttaac gtctttacgc ggctctttac caaagaactc
     2161 agcaactttc ttctgaacca ttggcatacg agtctgacca ccaacgagga taacgtcgtc
     2221 gatatcagat acggacaggc cagcgtcctg cagtgcaact ttcagcggct caatggaacg
     2281 gtttaccaga tcttcaacca ggctttccag tttcgcacga gtcactttga tgttcatgtg
     2341 tttcggaccg gtcgcatctg cagtgatgta cggcaggtta acgtcggtct gctgagcgga
     2401 agacagttcg attttcgctt tttctgccgc ttctttcagg cgctgcattg ccagcggatc
     2461 gttgcgcagg tcaatgccct gatctttctt gaattcttca accaggtagt tgatcagacg
     2521 gctgtcgaag tcttcaccac ccaggtgggt atcaccgttg gtagccagaa cttcgaaggt
     2581 tttttcgccg tcaacttcgt cgatttcgat aatagaaata tcgaaagtac caccacccag
     2641 gtcataaacc gcgatagtac ggttgccagt acctttgtcc agaccgtaag ccagcgcagc
     2701 tgcggtcggt tcgttgatga tacgttttac ttccagacca gcgatacggc ctgcgtcttt
     2761 ggttgcctga cgctgagcat cgttaaagta tgccggtacg gtgataacag cttcagttac
     2821 cggttcaccc aagtaatctt cagcggtttt cttcattttt ttcagcactt cagcagagat
     2881 ctgcggcggt gccattttct ggcctttaac ttcgacccat gcgtcgccgt tatcagcagc
     2941 aataattttg aacggcatga tggaaacatc acgctgtact tcttcgtcct ggaagcggcg
     3001 gccaatcagg cgtttaatcg caaacagggt gttttgcggg ttcgtcactg cctgacgttt
     3061 agccggctga ccaaccagag tttcaccatc ctgggtatag gcaatgatag aaggcgtggt
     3121 gcgatcgcct tcggcgttct ccagcacgcg aggagtggtg ccatccataa tcgctacaca
     3181 agagttggta gtacccaggt cgataccaat tattttaccc atctaaacgt ctccactata
     3241 tattcggtca tcatgtggtt gtgagtctgt aataggggcg aaacgtctgg tttcaactgc
     3301 ccaatttcat ttttttgcag actcactcac tgcggttgac tactaaatgg ggtcgtaacc
     3361 cacgtcatca agggggagat gcaaaaattt tttgtgcttt tgcgcagaaa tttcgcgata
     3421 tagcaggaca agacgtgaaa ctgacagcaa ttttattcat ataaagaatg aatcgcagcc
     3481 aagacattca tcaaaaaatt gtaataaaaa gaaaacatta cgcacctgaa tcttctcttt
     3541 atcagcagta aactagtggg tatccatccc cctacctctc cccactaaga gaatccttat
     3601 gaaatccgtt tttacgattt ccgccagcct ggcgattagt ctgatgctgt gctgcacggc
     3661 gcaggcaaac gaccataaaa tcctcggcgt cattgcaatg ccgcgtaacg aaaccaacga
     3721 tctggcgctg aaacttcctg tttgccgaat tgttaaacgc atacaactct ctgccgacca
     3781 tggcgattta cagttaagcg gtgcatcggt ttatttcaaa gccgcccgta gcgccagtca
     3841 gagcctgaat attccttcag aaataaaaga agggcaaacc actgactgga tcaacattaa
     3901 cagcgataac gacaataaac gctgcgtctc aaaaatcacc ttttccggtc atacggtgaa
     3961 ctcatcggat atggccacgc tgaaaattat cggcgacgac taacgccaga atatagaagc
     4021 cacaaaaaat gaatgttaat tacctgaatg attcagatct ggattttctc cagcattgca
     4081 gtgaggaaca gttggcaaat ttcgcccgat tgctcaccca taatgaaaaa ggcaaaactc
     4141 gcctctccag cgtactgatg cgcaacgaac tgtttaaatc gatggaaggg catcccgagc
     4201 aacatcgccg caactggcag ctgattgctg gagaattaca gcattttggt ggcgatagta
     4261 tcgccaacaa actgcgcgga cacggtaaat tgtatcgggc cattttgctc gatgtttcaa
     4321 aacgattgaa gctgaaagcc gacaaagaga tgtctacgtt tgagattgag caacagttac
     4381 tggaacaatt tctgcgtaac acctggaaga atatggacga ggaacataaa caggagtttc
     4441 tgcacgcggt cgatgcgagg gtgaatgagc tggaagagct gctgccgctg ctgatgaaag
     4501 acaaattatt ggcaaaaggc gtgtcgcatt tgctttccag ccaactgacc cgcattttac
     4561 gcacccacgc agcaatgagc gtacttgggc atggtttgct gcgcggcgcg gggctgggag
     4621 gccctgtagg tgcggcacta aatggggtta aagcggtcag cggcagcgcc tatcgcgtga
     4681 cgattccagc cgtactgcaa atcgcctgcc tgcgccggat ggttagcgcc actcaggtct
     4741 gataccgtaa actggggaaa attttcattt tcccctcaaa agatcgtaga cactgcccca
     4801 ctggctgatt attatgccgc gccctgaaaa cactacagtt attcagggaa attatttcac
     4861 cattcattcg atgatgattt ttgaggaatt atgggcaaca ctaagttggc taatccggca
     4921 ccgctgggcc tgatgggctt cggcatgacc accattctgc ttaacctgca caacgtgggt
     4981 tatttcgctc tggacggtat tattcttgcc atgggcattt tctacggcgg catcgcgcaa
     5041 atttttgctg gtctgctgga gtacaaaaaa ggcaacactt tcggtttaac cgcattcacc
     5101 tcttacggtt ctttctggct gacgctggtt gcgattctgc tgatgccgaa actgggtctg
     5161 accgatgcgc caaatgcaca gttccttggt gtctacctgg gtctgtgggg cgtatttacg
     5221 ctgtttatgt tcttcggcac gctgaaaggc gcacgcgttc tgcaattcgt tttctttagc
     5281 ctgaccgtgc tgtttgccct gctggcgatc ggtaacattg ccggtaacgc cgcaatcatc
     5341 cactttgccg gctggattgg gctgatctgc ggtgccagcg caatctatct ggcgatgggt
     5401 gaagtactga acgagcagtt tggtcgcacc gttctgccga ttggtgaatc ccactaattg
     5461 ttttgagatc cccccgctcg gggggatttt tttattcgct aacgtcgcgt cttgcactct
     5521 tcggtctgaa tgctgcaacc acttccggtg ccgtttcaac gtatggccct tccagcaact
     5581 gaatgcagta cggtacgctg gcaaaaatac cgtgtaccac aacgttaccc gcagcgtcct
     5641 tcacaccttc cagcgtctct ttaatagact tcggctgacc gggtaagtta aggatcagcg
     5701 cctgtttgcg aatcacacca acctgacgcg aaaggatcgc agttggtaca aaatgcaggc
     5761 ttatctggcg catctgttca ccaaagccag gcatctcgcg gtccgctact gccagcgtcg
     5821 catcgggcgt tacgtcacga cgcgccgggc cagttccgcc cgtggtgagc accagatggc
     5881 aactcatttc atccactagc tcacacaacg tttgctcgat gatcgcctgc tcatcgggga
     5941 ttaagcgggt ttccagttca aacggcgtgg ttagcgccga tgtcagccat tcttccagcg
     6001 cagggatgcc tttatcctga taaacgccgc tggatgcgcg atcagagatg gaaactaagc
     6061 caatacgtaa agtattcatc aagatttttc cgtttaaaca gtctcgttaa acagaatgat
     6121 acactgcgaa gggagtgaca gacaggcttc agaagaggta gcgtgaccga cttcccggtc
     6181 acgctaagaa tgattacagc agatcgccga tcattttttc cagtttttcc tggtcaatag
     6241 caaacttacg gataccttcc gccagtttat ctactgccat tggatcctgg ttgtgctgcc
     6301 acaggaactc ggactcagtg atacgcgccg gacgcgcttt cacttcgccg gtgtaagaca
     6361 gtttacgttc gatagcccct tcgctctcag ccagctcttt cagcagtgta ggtgcgatgg
     6421 taagacggtc gcagcctgcc agttccagaa tttcgccgat gttacggaag cttgcgccca
     6481 taaccacggt ttcataaccg tgctctttgt agtactggta gatttcagat acagaaacca
     6541 cgcccggatc ttctgccgga gcgtactctt tcttatcagt attcgctttg taccagtcaa
     6601 gaatacggcc aacaaatggc gagatcagga acacgcctgc ttccgcacaa gcacgagcct
     6661 gagcgaagga gaacagcagg gtcaggttac agttgatgcc ttctttttcc agctgttctg
     6721 cagcacggat accctgccag gtagaagcca gtttgatcag aatacgatcg ttgctaatac
     6781 cagcatcgtt gtagagtttg atcaggcgtt ttgctttcgc aattgacgct tcggtgtcat
     6841 aggaaagacg cgcatcaact tcagttgaga tacgacccgg aaccagtttc aggatttcca
     6901 gaccaatatt tactgccagt ttgtcggtcg cgtccacgat ctgctgcgcg cgatcgttgc
     6961 tctgctgttt cgcccaggcg acagcatcat caatcaactt acggtattcc ggaatctgcg
     7021 ctgcgttaag aatgagagaa gggttggttg tggcatcctg cggttgatac agcttcattg
     7081 ccgcgatgtc cccagtgtcg gccactacgg tggtgtactg acgaagggag gtcaatttgt
     7141 ccgtcatgat agtatttctc tttaaacagc ttgttagggg gatgtaaccg gtctgccctg
     7201 atgatatcac gacgcaacgt cagcgcaacc gccgacaaga cggttatcgc caggcgagac
     7261 tgtttcggat ttctgacact gcgttgatat cgcccgccat ttttatacaa aacctcatgt
     7321 atgctacgca gaagttatca agtacctcgt agcgtatata cttcttaaac aattggtaac
     7381 gtttacacag gaaagtcatc gcgaccggca ataagaggga tatgcatgcc agattttttc
     7441 tcctttatta acagcgtcct ttggggatcg gtaatgattt acctgctctt cggcgcaggt
     7501 tgttggttca cttttcgcac cggatttgtg cagtttcgct acatccgcca gtttggcaaa
     7561 agtcttaaaa atagcattca tccacagcca ggcggtttaa cctcatttca gtcattgtgt
     7621 accagtcttg cggcgcgcgt gggtagcggc aacctggccg gcgttgcgct ggcaattacc
     7681 gccggtggac ctggagccgt cttttggatg tgggttgccg cgtttatcgg catggcgacc
     7741 tcgtttgccg aatgttctct ggcacaactt tataaagaac gtgacgtcaa tggacagttt
     7801 cgtggcggac cggcatggta tatggcgcgc gggctgggga tgcgttggat gggcgttctg
     7861 ttcgccgtct ttttgctcat cgcctatggc ataattttca gcggagttca ggcgaacgcc
     7921 gttgcccgcg ccctaagttt ttcttttgat tttcctccgc tggtgacagg cattattctc
     7981 gctgtctttg ctctgctggc aatcactcgc ggtcttcatg gcgtcgcccg gctcatgcag
     8041 gggtttgtcc cgttgatggc gataatctgg gtgctgacca gcatggtgat ttgcgtaata
     8101 aatatcgggc aacttcccca cgtcatttgg tctatttttg agagtgcttt tggctggcag
     8161 gaagcggcag gcggcgcggc gggatatacc ttaagccagg cgattactaa cggttttcag
     8221 cgcagtatgt tttccaatga ggcgggaatg ggttcgacgc caaacgcggc agcggcagcg
     8281 gcgtcctggc ctccgcatcc ggcagcgcaa gggattgtcc agatgattgg catttttatc
     8341 gacaccctgg tcatctgtac ggcaagcgcc atgctgatat tactggcggg taacgacaca
     8401 acctacatgc cgctggaagg tattcagctt atccagaagg cgatgcgggt gctaatgggt
     8461 tcctggggtg ctgagtttgt taccctcgtg gttattctgt ttgccttcag ctccatcgtt
     8521 gccaactaca tttatgccga aaacaatctc ttctttttac gcctgaacaa ccctaaagcg
     8581 atctggtgtt tgcggatctg caccttcgca acggtcatcg gcggcacctt gctaagcctt
     8641 ccgctgatgt ggcaactggc agatatcata atggcctgca tggctattac caatttgacc
     8701 gccattttac tgctctcgcc tgtggttcat accattgcca gtgattatct acgccagcgt
     8761 aaactcggcg tgcgcccggt gtttgatccg ttgcgttatc cggatatcgg tcgccagctt
     8821 tctccggacg cgtgggatga tgtttcgcag gagtaatcac aactatcgat caactcattc
     8881 tctttttttg ctaaagtcgg cataaatttc ctgcaaggac tggatatgct gattcttatt
     8941 tcacctgcga aaacgcttga ttaccaaagc ccgttgacca ccacgcgcta tacgctgccg
     9001 gagctgttag acaattccca gcagttgatc catgaagcgc ggaaactgac gcctccgcag
     9061 attagcacgc tgatgcgcat cagcgacaaa ctggcgggta tcaacgccgc tcgctttcat
     9121 gactggcagc cagatttcac gccggagaat gcccgccagg cgattctggc gtttaaaggt
     9181 gatgtctaca ccggcttgca ggccgaaacc ttcagcgaag acgatttcga ttttgcccaa
     9241 cagcatttgc gaatgctttc cggcttgtat ggcgtactgc gcccgctcga tttaatgcag
     9301 ccttatcgtc tggaaatggg gatccgtctt gagaatgccc gagggaaaga tctgtatcaa
     9361 ttctggggag atatcatcac caacaagctg aacgaggcgc tcgcagcaca aggcgataat
     9421 gtggtgatta atctggcgtc agatgagtat tttaaatcgg tgaagccgaa gaaattgaat
     9481 gccgagatta tcaagccggt gttcctcgat gagaagaacg gcaaatttaa gataatcagc
     9541 ttctacgcta agaaagcacg cggtctgatg agtcgtttta ttattgaaaa tcggctgacc
     9601 aaaccagagc aactgactgg ttttaatagc gaaggttact tctttgatga agcttcctcc
     9661 agcaatggcg aactggtgtt taaacgctac gagcagcgtt aagtttgtag tcaatgccgg
     9721 atgcggcgtg aacgccttat ccggcctaca aaagcatgca gattcaatat attgcagaat
     9781 tcacgtaggc ctgataagac gcgccagcgt cgcatcaggc gctgaatgcc ggattgttac
     9841 ccggcatttg tcatttagcg gtgatgtttg cctggaccat gaccgccgtg atgatcatga
     9901 ggagctttct tatggtggcg cggcggtggc ggcggtccgt gtgggtgcca gcgattgcct
     9961 cgccattcat aatgttgttt ccaccagccg tggtcgcgcc agtgacctcc atcccagtaa
    10021 tagccacgat tatcacgatc gcctatctgt aattttactg acgggactaa cgtaatttcc
    10081 gcagcctgtg ctgccatggg agcgaccaga accagggaaa gtgcgagtac gatagattgc
    10141 atctttttca caggttactc cttttatcat tgcccgttat gggtcgatat ccggagatta
    10201 cgcgatgaga acgacggttg ttattctccg caatcctaat ctctaagtta ccgcatttat
    10261 tgagaatttc tcctttttcc ctgtcgtttt tctccataat ttcttcataa aaaagccggg
    10321 ctgcataaaa gcaaacccgg cctgattgag ataatgaata gattttactg atgattcatc
    10381 atcaatttac gcaacgcagc aaaatcggcg ggcagattat gtgaaagcaa gggtaaatca
    10441 gcacgttctg ccagctcttt tggcagatcc aacgtttcac cgagaatcgc ttccacgctc
    10501 tctttaaatt tcgccggatg cgcggtgccg aggaacaagc catattcgcc tggattcaac
    10561 tggtcacgca gcgcacgata cgctacggca gcgtgcggct ccgaggtgta gcccagttct
    10621 tttaactcac gcattgtctg ttgcgtggtt tcatcatcca cggctgcata acccagctct
    10681 ttcagttgcc agattttgcg gcggaacaac tcttccacac gcggccagtt gttcggctga
    10741 ctcacgtcca tcgcgttgga taacgtcgcc tgagtcgctt tgggtgacca ctgaccgtcg
    10801 tgcaggaaac gtggcacggt atcgttcacg ttggtcgcag caataaaacg tttcaccggc
    10861 agaccgagtg acttcgccag cagacccgcc gtcaaatcgc cgaagtttcc gcttggcacc
    10921 gagacaacca gctggttgcg cgcttcctgc ggcagctgcg caacagcttc aaagtagtag
    10981 caaatctgcg ccagcaaacg gctgatgtta atcgagttag ctgagtttaa ccccagcgcc
    11041 actttcagtt cttcatcatc aaacgcctgc ttcaccagcg cctgacaggc atcgaaatcg
    11101 ccgtcgatgg caacagtttc gatattgccg cccaacgtac agaacagttt ttcttgcagt
    11161 ggactgattt tgcctcgtgg atagaggata accactttca cattcggtaa accgtagaaa
    11221 gcatgagcca ctgccgctcc ggtatcaccg gaggtcgcgg tcagaatggt cactggctta
    11281 tcgcccgcaa tatgggtcag catttgtgcc ataaagcgac cgccgaaatc tttaaatgcc
    11341 agcgttggcc cgtggaacaa ttccagacaa ccgacatcgc tttcaacatt ggcgaccgga
    11401 gccgggaagg caaacgccgc gcgcacgcgc tcttccagga tttcctgcgg gatttcatca
    11461 ccaataaacg ccgagaggat cttcgcactg cgggtgacaa aatccagctt cagcatctca
    11521 tcaatttcag tcaggctgaa ttccggcagg tcatgcggaa aaaacagccc ctgatttttg
    11581 cccaacccct gggttacggc ttgcgcaaag ctgacctgct cgttgtgatc tttcagattg
    11641 tagagtttca tttagttttc cagtactcgt gcgcccgccg tatccagccg gcaaatatga
    11701 acaaaacctt cctgattttg caggtagttc ttacccaacc agtcggcaac gcgctgggcg
    11761 gtatccggct tgtcacatag agcgaacaag gtcgggccgg agccggagat accgctcgct
    11821 accgcgccga tttccgcgac cgcctgccgc gcctgccgga agccaggcag taaccgttca
    11881 cggtagggtt cagcgataac atctttcatc agcttcgcgg caagctcagg ctgacgggaa
    11941 tagcaggcgt gaatgaagcc agccagatgt cgcccgtgcg caatgcaatc ctggcggcga
    12001 tactgcgccg gtaaaatagc cctggcttct gccgtcgaga ctttaatccc cggatacgcc
    12061 agcacccaca gccactcatc aaaccctggc acttgctggc tgatgatgtc gttttcttcg
    12121 atcatcaact gcataccacc aagaaaacac ggtgccacgt tgtcgtaatg aatgctgccg
    12181 gagattcgtc cttccagctc gcccatcaaa gccagcaaac gagtgtcatt aagcggcttg
    12241 ccacagtgtt cattcatcgc catcagcgcc gcgaccaccg aacaggcgct ggagcctaag
    12301 cccgaaccga ttggcatatt cttttccaga gtcatcgcca ctggaatttg cttgccaagc
    12361 tcctggcaaa aacgctccca gcactgataa acgatatttt cccgtggttc tgacggcagc
    12421 ttatcggcaa agcgtccgag gttgttgaga ctgaatgtct ctgccgcctc aaccgtgact
    12481 acatctccga gcaatgcacc atcaacaggt gtcaccgccg ccccgagcac atcaaacccg
    12541 acgctcatat tggcactgga agccggggca taaactttaa ccatgtcaga ctcctaactt
    12601 ccatgagagg gtacgtagca gatcggcaaa gacaccggca gctgtaacgt cattgcccgc
    12661 accatatccg cgcagcacca acggcagcgg ctgataatag tggctataaa aggccagggc
    12721 gttttcgcca tttttcactt tgaacagcgg atcattacca tccacttcgg caatcttcac
    12781 gcggcaggca ccatcttcat caatattgcc aacatagcgc aaaacttttc cttcatcacg
    12841 ggccttcgcc acgcgcgcgg caaagagatc gtcgagctgt gacagattcg ccataaaagc
    12901 ggcaacatca ccctcagcgt taaactctgc gggcagcaca ggttcaattt caatatccgc
    12961 cagctccagt tcacgtcccg tttcacgagc gagaatcaat agcttacgcg ctacatccat
    13021 accagaaaga tcatctcgcg gatccggttc ggtataaccc atttcccgcg ccagagtagt
    13081 cgcctcggag aaactcatgc cttcgtctaa cttgccgaag atataagaaa gcgaacctga
    13141 aagaatgccg gagaacttca tcaattcatc accagcattg agcagatttt gcaggttctc
    13201 aataaccggt aatccagccc caacgttggt gtcatagagg aatttacgcc gcgatttttc
    13261 cgccgcatga cgcaacagat ggtagtaatc catcgacgag gtgttggcct ttttgttcgg
    13321 cgtgacaacg tggaaacctt cgcgcaagaa gtcggcatat tgatccgcca ctgcctggct
    13381 ggaggtgcag tcaacaatga ccgggttcag cagatgatat tctttcacga ggcgaattaa
    13441 gcgcccgaga ttaaacggct ctttggcttg cgccagttct tcctgccagt tttccagatt
    13501 taggccatgc acattggtga gcagtgcctt cgagttggca acaccgcaga cacgtaagtc
    13561 gatatgttta ttcttcagcc agctttgttg acgcttcagt tgctccagca gcgcaccgcc
    13621 aacgccaccg acgccaatca caaacacttc gataacctga tcggtattga acagcatctg
    13681 atgagtaacg cgcacgccag tggtcgcatc atcgttattt accacgacag agattgagcg
    13741 ttcagaagat ccctgagcaa tggcgacaat gttgatattg gcgcgggcca gcgcggcaaa
    13801 gaatttcgcc gagatcccac gcaaggtgcg cataccatca cctaccaccg agataatggc
    13861 cagccgttcc gtcaccgcca gcggctccag taagccttct ttcagttcca ggtagaactc
    13921 ttcctgcatt gcccgttcag cccgcacaca gtcgctttgc ggaacgcaga aactgatact
    13981 gtattcggaa gatgattgcg taatcagcac cacggaaata cgggcgcgtg acatcgcggc
    14041 aaagacgcgc gccgccatgc cgaccattcc tttcatcccc gggccggaaa cgctgaacat
    14101 tgccatgtta ttcagattgg aaatgccctt gaccggtaat tcgtcttcat cacgactggc
    14161 accaatgagc gtacctggtg cttgaggatt tccggtattt ttaatcaggc aagggatctg
    14221 gaactgggcg atgggggtaa tggtgcgggg gtgaagaact ttagcgccga agtaggaaag
    14281 ctccatcgct tcctggtagg acatcgactt caacaacctc gcatcgggca cctgacgcgg
    14341 gtcgcaggta tagaccccgt caacgtccgt ccaaatctcg caacaatcgg cgcgtaaaca
    14401 ggcagccagc accgccgcgg agtagtcgga accgttgcgt ccaagtacca ccagttcgcc
    14461 tttttcatta ccggcggtga aacctgccat caacaccatg tgatcagccg gaatgcggct
    14521 tgccgcaata cggcgggtgg actcagcaat atcgacggta gattcgaggt aatgccccac
    14581 tgccagcagt ttttcgaccg gatcgataac ggtaacgttg tgaccacgcg cttctaacac
    14641 gccggccata atggcgatcg acattttctc gccacggcaa atcagcgcag cgttgatgct
    14701 atccgggcac tgccccaaca aactaatgcc atgcaggaca tgttttattt gggcaaattc
    14761 ctgatcgacg aaagttttca attgcgccag cgggaacccc ggctgggcgg cggcgagtcc
    14821 cgtcaaaagt tcggcaaaaa tacgttcggc atcgctgata ttgggtaaag catcctggcc
    14881 gctaatggtt ttttcaatca tcgccaccag gtggttggtg attttggcgg gggcagagag
    14941 gacggtggcc acctgcccct gcctggcatt gctttccaga atatcggcaa cacgcagaaa
    15001 acgttctgca tttgccactg atgtaccgcc gaacttcaac actcgcatgg ttgttacctc
    15061 gttacctttg gtcgaaaaaa aagcccgcac tgtcaggtgc gggctttttt ctgtgtttcc
    15121 tgtacgcgtc agcccgcacc gttacctgtg gtaatggtga tggtggtggt aatggtggtg
    15181 ctaatgcgtt tcatggatgt tgtgtactct gtaattttta tctgtctgtg cgctatgcct
    15241 atattggtta aagtatttag tgacctaagt caataaaatt ttaatttact cacggcaggt
    15301 aaccagttca gaagctgcta tcagacactc tttttttaat ccacacagag acatattgcc
    15361 cgttgcagtc agaatgaaaa gctgaaaaat acttactaag gcgtttttta tttggtgata
    15421 tttttttcaa tatcatgcag caaacggtgc aacattgccg tgtctcgttg ctctaaaagc
    15481 cccaggcgtt gttgtaacca gtcgaccagt tttatgtcat ctgccactgc cagagtcgtc
    15541 agcaatgcca tgactcgttc gcgtaaagct tgcagttgat gttggtctgc cgttgtgtca
    15601 cttttcgccg gttgttgtat taatgttgct aattgatagc aatagaccat caccgcctgc
    15661 cccagattga gcgaaggata atccgccacc atcggcacac cagtaagaac gtcagccaac
    15721 gctaactctt cgttagtcag cccggaatct tcgcgaccaa acaccagcgc ggcatggctc
    15781 atccatgaag atttttcctc taacagcggc accagttcta ctggcgtggc gtagtaatga
    15841 tatttcgccc gactgcgcgc agtggtggcg acagtgaaat cgacatcgtg taacgattca
    15901 gccagcgtcg ggaaaacttt aatattatca ataatatcac cagatccatg tgcgacccag
    15961 cgggtggctg gctccaggtg cgcttgacta tcgacaatcc gcagttcgct aaaacccatc
    16021 gttttcattg cccgcgccgc tgccccaata ttttctgctc tggcgggtgc gaccagaata
    16081 atcgttatac gcatattgcc actcttcttg accaaataac cgcgaacagg gtgatcactg
    16141 tcaacttatt acgcggtgcg aatttacaaa ttattaacgt aagtcgcaga aaaagccctt
    16201 tacttagctt aaaaaaggct aaactatttc ctgactgtac taacggttga attgttaaaa
    16261 aatgctacat atccttctgt ttacttagga taattttata aaaaataaat ctcgacaatt
    16321 ggattcacca cgtttattag ttgtatgatg caactagttg gattattaaa ataatgtgac
    16381 gaaagctagc atttagatac gatgatttca tcaaactgtt aacgtgctac aattgaactt
    16441 gatatatgtc aacgaagcgt agttttattg ggtgtccggc ccctcttagc ctgttatgtt
    16501 gctgttaaaa tggttaggat gacagccgtt tttgacactg tcgggtcctg agggaaagta
    16561 cccacgacca agctaatgat gttgttgacg ttgatggaaa gtgcatcaag aacgcaatta
    16621 cgtactttag tcatgttacg ccgatcatgt taatttgcag catgcatcag gcaggtcagg
    16681 gacttttgta cttcctgttt cgatttagtt ggcaatttag gtagcaaaca tgcagacccc
    16741 gcacattctt atcgttgaag acgagttggt aacacgcaac acgttgaaaa gtattttcga
    16801 agcggaaggc tatgatgttt tcgaagcgac agatggcgcg gaaatgcatc agatcctctc
    16861 tgaatatgac atcaacctgg tgatcatgga tatcaatctg ccgggtaaga acggtcttct
    16921 gttagcgcgt gaactgcgcg agcaggcgaa tgttgcgttg atgttcctga ctggccgtga
    16981 caacgaagtc gataaaattc tcggcctcga aatcggtgca gatgactaca tcaccaaacc
    17041 gttcaacccg cgtgaactga cgattcgtgc acgcaacctg ctgtcccgta ccatgaatct
    17101 gggtactgtc agcgaagaac gtcgtagcgt tgaaagctac aagttcaatg gttgggaact
    17161 ggacatcaac agccgttcgt tgatcggccc tgatggcgag cagtacaagc tgccgcgcag
    17221 cgagttccgc gccatgcttc acttctgtga aaacccaggc aaaattcagt ctcgtgctga
    17281 actgctgaag aaaatgaccg gccgtgagct gaaaccgcac gaccgtactg tagacgtgac
    17341 gatccgccgt attcgtaaac atttcgaatc tacgccggat acgccggaaa tcatcgccac
    17401 cattcacggt gaaggttatc gcttctgcgg tgatctggaa gattaatcgg ctttaccacc
    17461 gtcaaaaaaa cggcgctttt tagcgccgtt tttatttttc aaccttattt ccagatacgt
    17521 aattcatcgt ccgttgtaac ttctttactg gctttcattt tcggcagtga aaacgcatac
    17581 cagtcgatat tacgggtcac aaacatcatg ccggccagcg ccaccaccag cacactggtt
    17641 cccaacaaca gcgcgctatc ggcagagttg agcagtcccc acatcacacc atccagcaac
    17701 aacagcgcga gggtaaacaa catgctgttg cgccagcctt tcaataccgc ctgcaaataa
    17761 ataccgttca ttaacgcccc aatcagactg gcgattatcc atgccccggt aaaaccggta
    17821 tgttcagaaa gcgccagcaa gagcaaataa aacatcacta atgaaagccc caccagtaaa
    17881 tattgcattg ggtgtaaacg ttgcgcggtg agcgtttcaa aaacaaagaa cgccataaaa
    17941 gtcagcgcaa tcagcagaat agcgtactta gtcgcccggt cagttaattg gcattgatcg
    18001 gctggcgtcg tcactgcgac gctaaacgcg gggaagtttt cccagccggt atcattgcct
    18061 gaagcaaaac gctcaccgag attattagca aaccagctgc tttgccattg cgcctgaaaa
    18121 cctgactcac taacttcccg tttggctggc aaaaaatcac ctaaaaaact gggatgcggc
    18181 cagttgctgg ttaaggtcat ttcgctatta cgcccggcag gcaccacaga aagatcgccg
    18241 gtaccgctta aattcagggc catattcagc ttcaggttct gcttgcgcca gtccccttca
    18301 ggtaaaggga tatgcacgcc ctgcccgcct tgctctaacc cggtgccggg ttcaatggtc
    18361 agcgccgttc cgttaacttc aggcgctttc accacaccaa taccacgcgc atccccgacg
    18421 ctaatcacaa taaatggctt gcctaaggtg atatttggct cgtcgagttc gctaagacgc
    18481 gaaacatcga aatcggcttt tagcgttaaa tcactgtgcc agacctgacc ggtataaatc
    18541 cctatcttgc gttcttcaac gttctgattg ccatcaacca tcaatgactc aggtaaccaa
    18601 aaatggataa aacttcgttt ccgctccacg gttttatcgt cttcctgcgc cgtataaagc
    18661 tcggtcacag gaatagcgat gagcggccca acgagttttt gcggcccgct ggtactttgc
    18721 cgaatcgcat cttccacatc gctacggtaa tcggcacgtt cgacaatcac ttgccgaatc
    18781 agcattatcg gaatcaacaa cagcaatact gcaccaaaca aggtggtcat tttccagaac
    18841 aggggggatt tcaacatagt cttctccttt gcaatggcag caggatacga aggctatgtg
    18901 ggaagaattt gaagctatgt gaagtgacgg tgaagtcgaa gcgaggccag cacgccacct
    18961 tcctgcacgt tgcgcagcgt gacttcgccg ttaaacaaac gggcgacctc actgacgaac
    19021 gccaacccca gaccgctgct tttttgccca tttgcacgcg gcaaagagta aaagcgttca
    19081 aaaatacgtg acagcgcgta gtcaggaata ccactaccgg tatccagcac cttaagggcg
    19141 acgtgttcct gatccacttc ggcgcttagc gttatgcgac cgctctcggg ggtaaaatcg
    19201 atggcgttat ccagtaaatt ccccagcgcc tgctccagta acgccggttc agcagcaaca
    19261 ttaacctcag tgggcataac atgtagagtg atgttttttt ctgccaactg cacggtgcgc
    19321 gcttcgctga cgcggcgaaa taatgccgcc acatcaacaa cagtcagaac gacttcctga
    19381 cgattctcca gtcttgcctg gcgtagtaac gtttctacca atgcctgcat acgcgcattt
    19441 tgcgtcagaa tgttgtcggt aaaacgagcc accacttccg gtggcggacc ttcgcgtaaa
    19501 atttccgccg cgccacgaat cgccgccagt gggcttttta gctcatgggt taacgcataa
    19561 acatactgct caatatagtt tttcccttcc agcttcacgc gcatactttc cagcgcctgc
    19621 gcaagcttac gcaactcgct actgccgagt tcaggaagag gaacgggctt attgtcagtg
    19681 acggaatcgg catagcgagt gagccgggca atggagcggt tgatccacca aaccatgcct
    19741 gcgccaatca ccagtgcaat ccccagcaaa atggcgctgg cccataaaat tcgccgctcg
    19801 ctacgcttaa tgaccggagc catcgccgcg ttcggtttgc ctacgctcaa aacgccaata
    19861 agccgcgagc cgtccataat cggcgcggca acatacatca ccgaactttc gggatcggca
    19921 ggattttgca acgtgctgcg cgcaccatac tgaccacgca acgttagcca gacgtcattc
    19981 cagcgcgaat aatcctgccc aacggcttta tttgccgaat cgaacaatac tttaccctgc
    20041 gaatcggtca tatagacatg atattcattg cgcactttgt taatgccacc gatattggcg
    20101 cgaaacgggc gatgttgtag ctgattaaac gcctgcgcca gttgcccatg cgttgggtcc
    20161 ccagagagca aatcgggacg cgccagctcc gccagcaacg ttgcggtatc gattaacgtc
    20221 ccctccgttg ctcttcgcac gcccggttta acttctttga caaaaatagc cagtacgaac
    20281 caggccgcca ccgccaccag taaaaaatag cccagcagca accgcatgcc gatacgcatt
    20341 acaggcccct caggctatat cccatgccgc gatgagtatt aatcggtgaa agatcggggt
    20401 tgatggcgcg cagcttggca cgcaacgttt taatgtgggt atcaacggtg cgatcgtagg
    20461 tgtcctgcgc atcttcccac acgctatcca tcagttgctg gcgggaccag acgcggcctg
    20521 gcgacttgag taacgtcttc agcaataaaa actcataccg cgtcagcgtt aatggcgtgt
    20581 caaaccagct gatctgcgcc gcgggttcat tcaattcaaa atgaccaata cggatgacgg
    20641 gagacggcga cgagaacttc ttcacccgac gcagtaaggt gcgcaccctg gcgcacactt
    20701 cgcggggtga aaacggttta gccacatagt cgtcagcgcc aatttccagc ccaagcaggc
    20761 gatcgacctc ttcacttcgg gccgtcagga acagtacagg taacgccgga tggagcgcca
    20821 gtaactggcg gcataattca aagccgctga tatcaggcaa accaacatcg agaatcatga
    20881 cgtcgggaac ctgctggcga gctttatcca gcaccggcaa gcctcgctca aagacctcga
    20941 cggcaaaacc ttcctgctgc aacatgtaga ctagcgtgtc ggctatccct tgctcatctt
    21001 ccactaacca gaccgtttcc cgttgcataa atcgcctctg ttattgccgc cacggcatga
    21061 caggaaccgc gctaatcgcg tttttcggcg aaccttctac aactttgtcg gagtaagcca
    21121 gataagcgag cgcgttgcgt ttggcgtcat aaaagcgcac gacctgtaac gacttaaaga
    21181 ccagggacgt gcgttttttg aatactacct cgccctgagc tttgccgttt ttaatacgat
    21241 acgacagttc aatcggcccg acttgctgac aagaaatagc cgcatcggag gtatcttccg
    21301 ccagacccaa tcccccttta ataccacctg ttttcgcccg gctcacataa caggtgacat
    21361 ttttcacatc gggatcgtca aaggcttcca caacaatttt gtgatccggg ccgatcattt
    21421 taaatacggt gtcgaccgaa ccaatctctt ctgcatgagc caatggcccc agcattatca
    21481 ggcttaaaga caggatcaaa tgcttgtatt tcatattgtt accattttta tctattacac
    21541 gagcaattag ttcgtcacgg taaataacat attgattaag aacaaaatct caatactttt
    21601 atttccgtca agccctaaaa catactctac taagaaaaaa acactgaatg ctaaaacagc
    21661 aaaaaatgct attatccaat tacctgatgt caggtgctcg ttgttgaaag gatgaggata
    21721 ttttatggat caggccggca ttattcgcga ccttttaatc tggctggaag gtcatctgga
    21781 tcagcccctg tcgctcgaca atgtagcggc gaaagcaggt tattccaagt ggcacttaca
    21841 gagaatgttt aaagatgtca ctggccatgc tattggcgcg tatattcgtg ctcgtcgttt
    21901 gtcgaaatcg gcggtcgcac tacgcctgac tgcgcgtccg attctggaca tcgcgctgca
    21961 ataccgcttc gactctcaac agacatttac ccgcgcattc aagaagcagt ttgcccagac
    22021 tcctgcactt taccgccgtt ctcctgaatg gagcgccttt ggtattcgcc cgccgctacg
    22081 tctgggtgaa ttcacaatgc cagagcacaa atttgtcacc ctggaagata cgccgctgat
    22141 tggtgttacc cagagctact cctgttcgct ggagcaaatc tctgatttcc gccatgaaat
    22201 gcgttatcag ttctggcacg attttctcgg caacgcgccg accattccgc cagtgctcta
    22261 cggcctgaat gaaacgcgtc cgagtcagga taaagacgac gaacaagagg tattctatac
    22321 cactgcgtta gcccaggatc aggcagatgg ctatgtactg acggggcatc cggtgatgct
    22381 gcagggcggc gaatatgtga tgtttaccta tgaaggtctg ggaaccggcg tgcaggagtt
    22441 tatcctgaca gtatacggaa cgtgcatgcc aatgctcaat ctgacgcgcc gtaaaggtca
    22501 ggatattgag cgatactacc cggcagaaga tgccaaagcg ggagatcgcc caattaatct
    22561 gcgctgtgaa ctgctgattc cgatccgtcg ttaacgctgc aactcatcta atgcaggggc
    22621 gtccagatgc gagatgtccc ctgcggtttc aacaacccag ccagacgcca gccacaggct
    22681 ttcctgataa tccacgcgcg aaatagaaca gttacgcaga cgtaagcggc gctctgccca
    22741 tgctggtaat ccgagaatcg tactcaccag acatcccagt gcaataccat ggctcaccag
    22801 cagcggtcgg cttccctgcg gtaagtcccg gcaagattcc agtgccgcat tgacgcgatc
    22861 gctgagctcc tgcattgact cgccttcagg aatacgcccg tcaacggtgc cattgaccag
    22921 ctgccgacgc cagttctctt cttcttcggt cagagaatcg atatgtcttt tttccagcac
    22981 acccatgttt aattcacgca ggcgagaatc aaagatgatg tcacagccgc aggcctgggc
    23041 gatgatttcc gccgtacgcc gggtgcgtcc taaatcgcta ctgataatat gcgtaatgcc
    23101 aagctctttg gcacgggttg ccacctgcat cgcctgttgc tcacctttgg cggtcagcgg
    23161 gctgtcagac tggccctgaa tacgtcgctc ggcgttccac tgcgtttcac cgtggcggac
    23221 taggtatacc tgtaacatgc tttttttccg tatactgctg taacgttaat tttctgagta
    23281 atgctgatta tgcaccaagt tgtctgtgcg accaccaatc ccgctaaaat tcaggccatt
    23341 ctgcaggcat ttcacgagat cttcggcgaa ggatcctgcc atattgcatc cgttgccgtc
    23401 gagagcggtg taccggaaca gccctttggc agtgaggaaa cgcgcgctgg cgcacgaaat
    23461 cgggtagcaa atgcccgccg tttacttcca gaggctgatt tttgggtggc gattgaagct
    23521 ggcatcgatg gtgacagcac tttcagttgg gtggtgattg aaaacaccag ccagcgcggc
    23581 gaggcgcgtt cggctacttt gccgttacct gcggtgattc tgcagaaagt gcgtgaaggt
    23641 gaggcgcttg gtcccgtgat gtcgcgttat accggtattg atgagattgg tcgtaaggaa
    23701 ggggcgatcg gcgtgtttac cgccgggaaa ctcactcgcg ccagcgtcta tcaccaggcg
    23761 gtgatcctgg cgctgagtcc gtttcacaat gccgtgtatc aggcattgta ggacggataa
    23821 ggcgttcacg ccgcatccgg cattcggtgc acgatgcctg atgcgacgct gccgcgtctt
    23881 atcaggccta caaaatcaat cgcttttcag caacacctct tccagccact ggcgcagctc
    23941 cacgggcgcg gctttcaggc tgttagatcc acgcgtaatc gtcgcgatgc ccgcgccgag
    24001 ttcatttttt aactcacgct ggctcatttc gccgcgcaac agctcttcga caatacgcac
    24061 ccgagtcccc aacgcttcgc gctcatctgg cgtcagcatc agatttaaca acggtaaatg
    24121 gagatcgttt tggtaggcat tcttaagcag gtcgacaaaa cgtaaccact cctggtgacg
    24181 ctgttctgcc atcgctgctg aatagggtga ttgttgggcc ataatatgtc gccattgtta
    24241 gcgggggaag caaaatgcct cccccggttg tactcgctaa agagtacgat agcatatcat
    24301 aaacgtgcgg atcagtaacg acgtccccat tccgtggcgc tcatcaacgt cggtttatcc
    24361 cccatgaaat agcggtagta agcgtcataa gccagcacgt tcttcacata accgcgcgtc
    24421 tcggagaacg gaatactctc gacaaatgcc acagcgtcga tacgcccggc gctgttgcca
    24481 agccaggttc gcacccgtcc aggtccggcg ttataagctg ctgaggagaa aatacggtta
    24541 ttgccaaact gctgataaac atattgcagg taactggtgc caatgttgat attcgtttcc
    24601 ggatccagca attgcccagg gctgctataa ccaggaatag agaacatctt caccgtatgg
    24661 gccgctgtac caggcataat ctgcatcagg ccgctggccc ctaccggtga tttcactttc
    24721 ggattccagg cgctctcctg acgggcaatc gccatcgcat agctttgcgg gatctctttc
    24781 ccactggtgt aacgtttgaa aagatcgttg taagccagcg ggaatcgctc ttccagatga
    24841 tcccaaagct tcccggcgat cgttgcctga acgctaagat cccaccattg gttattgaaa
    24901 gcataccgag ccagttgagc ctgctctgtt tttgacttgc tcttcaccag attggcccac
    24961 tcgctacgcg cggtgttatc gagattccag tacatcaact cgcgcacgcg cgccatctcc
    25021 ggcccctgag tcagggcgct gtcaacattc tgcggcgctt tatcaatctt cagctcatac
    25081 tcttctccga tgcgttgtgc tgcaaccatc gggtagaaac cacgctgttg catgagttga
    25141 tgcaaaatct ctttcgcttc agcttcacgt ccgcgttcca gcaataaatc cgcctgccag
    25201 taacgccatt catctttctc ttttgcttcc atcggcagac gcgccagcca ggtattcagg
    25261 ccgcgacgat cgccggtgcc aagtgccatt cgcacacggc gttcaatcaa cgacgtggat
    25321 tgcgagcgca taatggcgtc atcgcgccat ttcgcctgct cgtcggtgac atcgttgccc
    25381 atcaaacgcc aggcgacgat atcgcgcagc tcctgaatct gatcttcatt aagctgctgc
    25441 gcctgggcaa gcgacgggat catcagccgt gcattttcag catcctgccg cgccacactg
    25501 gcaaacgcca ccgccgccat ttgacgggta aaatcggtcg ctccggttgt acgcgcgaag
    25561 gtcagtaccg tattagggtt gttcgccagt gaaatgattg ccgaggcgat agtctggtaa
    25621 tcggcaggca tctgccctgc cagcactgtt accaggccag tgttacccgc tttcatcgcc
    25681 agacggatac gctctaaata cgccagcgga tcttgtttac ctgacgcacg ccagacgcta
    25741 aataacttgt cacaggcgtt aggctggctc ttgccggtta gccacagctc tttcgcccct
    25801 tgccaggctt cttcactctg cccggtgttc catttcgcat agtagtaatt acattgcgct
    25861 tcggtagttc cgggcttttc cgggctaaag gctaacaagc cacgccagtc ttcacgccgc
    25921 gccagttcat tgacgaaacg agattgcagc gtgcgagcgg gaggaagcgt ggggttagcg
    25981 cgaacaaagt tagtgaccgt caccgccggt tgattcatca gatcatcggt gatctggcgg
    26041 tattccaggt agggataaag cggataatcc ttcagtccag gcatcatttg ttcgaccaca
    26101 tccatttgtc gattatccca ggcctgcttg atttgcgcgt aacggctacg ctgctcatcc
    26161 agtgagtcgg ctcgcgccac gctgctgacc gtcagcagac agacaccggc agccaacagc
    26221 cgccaggtaa cttgtttggc tttttccaca agcgcatcct ctaagtgtaa atacatcaat
    26281 gcagcgtcat gccgcgtaat agatttatgc taaccagtca ttgccgttta cgccacgtta
    26341 cggacacttt tttactttta ctgcgaaggt gatcgaccaa ctgggattag gcggggaaaa
    26401 aggctacact tcgcctttga aaactcatca tcacgataaa catagaggcg aagtccaacg
    26461 tggctcaatt cgtttatacc atgcatcgtg tcggcaaagt tgttccgccg aaacgtcata
    26521 ttttgaaaaa catctctctg agtttcttcc ctggggcaaa aattggtgtc ctgggtctga
    26581 acggcgcggg taagtctacc ctgctgcgca ttatggcggg cattgataaa gacatcgaag
    26641 gtgaagcgcg tccgcagcca gacatcaaga ttggttacct gccgcaggaa ccgcaactga
    26701 acccggaaca caccgtgcgt gagtccattg aagaagcggt ttctgaagtg gttaacgccc
    26761 tgaaacgcct ggatgaagtg tatgcgctgt acgccgatcc ggatgccgat tttgacaagc
    26821 tggccgctga acaaggccgt ctggaagaga tcattcaggc tcacgacggt cataacctga
    26881 acgtacagct ggagcgtgcg gcggatgcgc tgcgtctgcc agattgggac gcgaaaatcg
    26941 ctaacctctc cggtggtgag cgtcgtcgcg tggcgttgtg ccgcctgctt ctggaaaaac
    27001 cagacatgct gctgctcgac gaaccgacca accacctgga tgccgaatcc gtggcctggc
    27061 tggaacgctt cctgcacgac ttcgaaggca ccgttgtggc gattactcac gaccgttact
    27121 tcctcgataa cgttgcaggc tggatcctcg aacttgaccg cggtgaaggt attccgtggg
    27181 aaggcaacta ctcctcctgg ctggagcaga aagatcagcg cctggcgcag gaagcttcac
    27241 aagaagcggc gcgtcgtaag tcgatcgaga aagagctgga gtgggtacgt cagggaacta
    27301 aaggccgcca gtcgaaaggt aaagcacgtc tggcacgctt tgaagagctg aacagcaccg
    27361 aatatcagaa acgtaacgaa accaacgaac tgtttattcc acctggaccg cgtctgggcg
    27421 ataaagtgct ggaagtcagc aacctgcgta aatcctacgg tgatcgcctg ctgattgatg
    27481 acctgagctt ctcgatcccg aaaggggcaa tcgtcgggat catcggtccg aacggcgcgg
    27541 gtaaatcgac cctgttccgt atgatctctg gtcaggaaca gccggacagc ggcaccatca
    27601 ctttaggtga aacggtgaaa ctggcatcgg ttgagcagtt ccgtgactca atggataaca
    27661 gcaaaaccgt ttgggaagaa gtttccggcg ggctggatat tatgaagatc ggcaacaccg
    27721 agatgccaag ccgcgcctac gttggccgct ttaactttaa aggggttgat cagggtaaac
    27781 gcgttggtga actttccggt ggtgagcgcg gtcgtctgca tctggcgaag ctgctgcagg
    27841 ttggcggcaa catgctgctg ctcgacgaac caaccaacga cctggatatc gaaaccctgc
    27901 gcgcgctgga aaacgccctg ctggagttcc cgggctgtgc gatggttatc tcgcacgacc
    27961 gttggttcct cgaccgtatc gccacgcaca tcctggacta ccaggatgaa ggtaaagttg
    28021 agttcttcga aggtaacttt actgagtacg aagagtacaa gaaacgtacg ctgggcgcag
    28081 acgcactgga gccgaagcgt atcaagtaca agcgtattgc gaagtaatgc gtaaaatgcc
    28141 ggatgcggcg tgaacgcctt atccggccta caaaccaaga aatttcaata cgttgtgaat
    28201 ctccaggcct gataagacgc ggtaagcgtc gcatcaggca ttgagcaccg aatgccggat
    28261 acggcgtgaa cgccttttcc ggcctacaac agccttagta tccgcaaatt cgtagcgggt
    28321 cacccgtgct gattctttta aaccttgttt aatctcgaca gcctcttcca gagaggtttt
    28381 caactcatca aaaaacttat tttatacctc gcctttgagc cgtttcatcg cggttatctc
    28441 tgctccccca tcatctcccg caccagctcc acgcagcgca ggaaacggct gtcataatcg
    28501 tcctcttcaa cccgcacgaa ttcgatattg ttctcttcca gcatctccac cagcaagttc
    28561 tggaactctt tgcgatccac cgaactgccg aggctgcgta aaccatccgc cacccacggc
    28621 gtgttgttct ccagcaggat caccagatcg aaacggtatt catcaatcaa cgcctgtacg
    28681 aacggatgct cacgcccttc gtactttttg cagaacgcct gggtagtgac aaaatcagtg
    28741 tcaataaacg ccactttatt ggcatatttc actgcaaaat caatgtattg tgcgtggccc
    28801 agcgcgattt tatcgtaatc ggaatactgc aatgcgatct catcaccgcc gaggtgtgaa
    28861 aagacataat cgcgaccata ttcccacgca ctggtggtat tgaagatatt ggcaagtttg
    28921 tttaccaaag tggatttacc gctcgactcg ccaccaagga tcgccacggt acgtacaaag
    28981 aacggcttca cttccgtagg aatatattcc cagtagcgga acgggttttc gcggatctgc
    29041 gcaccgctga tactcataaa ggtacgtttc ggatcgacca gcaccgtatc gatccccaga
    29101 tgttccatat actgtggcgc atcggcttct tccgaggtgt agatcaggtc cggctgaatc
    29161 cccttttcag ccataaactt tttgatgccg ttgctccaca catcccagcc gtgcggatac
    29221 ggctccatgc cctcttcgtt gaaagcatga atgcgaatat ttttctgata tttaaaagtt
    29281 tgtaataacc agcgcagacg atccggcacc gtgggctgct gcgacatggc gctgtcttca
    29341 aacaacgcgc ggtcgcgggt atcgtcaaaa cccataatga tatgcagctc gtcaacctgg
    29401 ctacaggcgc gctggataag gtagatatgt ccggtatgca gcgggtagaa cttaccgaat
    29461 acgacaccaa tggttttctt ctgtcgggga aattcaagcc ccaaaaaacg gtgcaacgcc
    29521 tccagctttt gcgcgctggg gcttttaatt ttggcattca gtaactggct taaataccct
    29581 ttggtcatac cgctggcatc agctacctgc tgtagcgtgc agccctgttg cttgatggca
    29641 gttttcaggt aatcaaatga cgacatatct ccctccgtat ctctcattat aagtcgtcga
    29701 acacgctaag cgcgtcggag agttttttaa cgccaaaaat ctgcatccct tccggcgctt
    29761 ttttcggtac gttagccgcc ggaacaatcg cccggcgaaa accgtgtttc gccgcttctg
    29821 aaattcgttc ctgaccgctg ggcaccgggc ggatctcccc tgccagcccg acttcaccaa
    29881 acaccaccag atcctgtggc agcggtctgt cacgcaggct ggaaaccatc gccagcagta
    29941 acgctaaatc ggcactggtt tcggttacct tcacgccgcc gaccacgttc acaaatacat
    30001 cctgatcggc catttgcaga ccaccgtgac ggtgcaagac agccagcagg attgccagac
    30061 ggttttgttc cagccccact gccacgcggc gcggattcgc catcatcgag tgatcgacca
    30121 gcgcctgaat ctccaccagc aacggacgcg ttccttccca caccaccatc acggagctgc
    30181 cggaggtcac ttcatctccg cgacttaaga aaattgccga agggttgctg acttcacgca
    30241 gcccctgttc ggtcatcgcg aagacgccca gctcattcac cgcgccgaag cggtttttat
    30301 ggctgcgcaa ggtgcgaaaa cgggagtcgg catcaccatc cagaagcacc gaacagtcga
    30361 tacagtgttc cagcacttta gggccagcca gcgagccatc tttggttacg tgcccaacca
    30421 tgacaatcgc cacaccgcgc gttttggcga agcgcgtcag ataagccgcc gtttcacgca
    30481 cctgcgccac gctaccaggc gatgattgta catccgccat atgcatcacc tggatcgagt
    30541 caattaccat cagtttcggt tgctcttctt cggcaatcag gcagatctgt tcgatgctgg
    30601 tttccgacaa catattgaga ttgtcagtcg gcaggccaag gcgatgagcg cgcattgcca
    30661 cctgttgcag cgactcttcg ccagtgacat acagcgtttt catctgctgg gccagtttgc
    30721 acagcgtttg cagtagcagc gtggatttcc ccgcacctgg gttaccgcca atcagaatgg
    30781 cgcttcctgg caccacgccg ccgcctagta cgcggtcgaa ctctttaaat ccggtggaaa
    30841 aacgcggcag ctcttcaagg ctgatatcgg agagtttctg gacttttgcc accccggcgc
    30901 taccggcata gccgctgaga cgctcgttac gcgccaccgt tggcgacgca gcaagacgca
    30961 cctcggtgat ggtgttccag gcatgacagg cactgcactg cccctgccag cgcggataat
    31021 cggccccgca ttcattacaa acaaaggcgc gttttggagc ttttgccacg gtgtacctcg
    31081 ttaatgctgt gccgcccgca ggatggcggg cgagcaatta cttctgattc aggctgccag
    31141 agaggatgca gaataccccc atcaggtcag cgtgacggat ggtgacttcc gccttttcat
    31201 tcacttttgg cttggcatgg taggcaatcc ccagccctgc tgctttgatc atcggcaggt
    31261 cattagctcc atcgccaatc gccacggtct gcgccagcgg gatttcatac tcctgcgcga
    31321 ggcgagtcaa agttttcgct ttgtactgcg cgtctacgat gtcgccgatc acattgccgg
    31381 taaatttacc gtccatgatc tccagttcat tggctaccac ggcggtcagg cgcagcttgt
    31441 cgcgcagata ttcagcaaag aaagtaaagc cgccggaggc aatcgccact ttccagccca
    31501 gcgtttccag cttgagcacc agttgcgtta agcctggcat cagcggcaga ttttcacgca
    31561 cctgttgcag aatattggcg tcagcgcctt tcagcgtcgc cacacggctg cgcaggctgg
    31621 cggtaaaatc gagttcgccg cgcatcgccc gttcggttac ttccgccacc atctcgcccg
    31681 ttccggccag tttggcaatt tcatcaatac attcaatctg gatggcggtg gaatccatgt
    31741 ccatcaccag caaacccggc gtgcgcaggt gcgggatttt ccccagcggg gcgacatcca
    31801 gttgcgcttc atgggccagg cgcgtagccc gtgcggtgag tgaacctgcc agacgaatca
    31861 cctgataatc ttccacgcac caggcggcaa caatcaccat cgccgcaccc agtttgctct
    31921 ggtattgggt cagacgctgt ttatccagcc cacgaccata cagcagccag ccgctacgac
    31981 ctgcgtggta atccagtggc atcacttcat caccacttaa tgaaagaggc agaccaggcc
    32041 ataaagagac atcttcaggc aggtcgcacc aggtaatgtt aggcattaag gctcctgtaa
    32101 aatcgttcga agcagggaaa ataacgcatg aggctacctt gtatccattg cttctggcaa
    32161 cattaagtct caaattttca aagggtggaa gatggctcgc acaaaactga aattccggct
    32221 gcatcgggca gtgattgtcc tgttctgtct tgccttgtta gtggcgctga tgcagggagc
    32281 gtcatggttt agtcaaaacc accagcgaca acgtaatcca cagctggaag aactggcccg
    32341 caccctggcg cgtcaggtaa cgctgaacgt tgcaccgctg atgcgtaccg actcaccgga
    32401 tgaaaaacgc attcaggcga tcctcgatca gttaacggat gaaagccgta tcctcgacgc
    32461 gggtgtgtat gacgaacaag gcgatcttat cgcacgttct ggcgaaagcg tcgaagtgcg
    32521 cgaccggctg gcgctcgacg gtaaaaaagc aggcggctat tttaaccagc agattgtcga
    32581 gccaattgcg ggtaaaaacg gaccgctcgg ctatctgcgc ctgacactcg acacccatac
    32641 gctcgccacc gaagcccaac aggtggataa caccactaac attttacgcc tgatgttgct
    32701 gctctcactg gcaatcggcg tagtgctgac ccgcacgctg ctacagggta aacgcacccg
    32761 ctggcagcaa tcgcccttcc tgttaaccgc cagcaaaccg gtgccggaag aggaagaaag
    32821 cgagaaaaaa gagtgaccca ttactacaag aaaggaaatc gttatgtcca cattacgcct
    32881 gctcatttct gactcttacg atccgtggtt taacctggcg gtggaggaat gtatttttcg
    32941 ccagatgccc gccacgcagc gcgttctgtt tctctggcgc aatgccgaca cggtagtgat
    33001 tggtcgcgcg cagaacccgt ggaaagagtg taatacccgg cggatggaag aagataacgt
    33061 ccgcctggcg cggcgcagta gcggtggcgg cgcggtgttc cacgatctcg gcaatacctg
    33121 ttttaccttt atggctggca agccggagta cgataaaacc atctctacgt cgattgtgct
    33181 caatgcgctg aacgcgcttg gcgtcagcgc cgaagcgtcc ggacgtaacg atctggtggt
    33241 gaaaaccgcc gaaggcgacc gcaaagtctc aggctcggcc tatcgcgaaa ccaaagatcg
    33301 tggcttccac cacggcacct tgctgctcaa tgccgacctt agccgcctgg caaactatct
    33361 caatccggat aaaaagaaac tggcggcgaa aggcattacc tcagtgcgtt cccgcgtgac
    33421 caacctcacc gagctgctgc cggggatcac ccatgagcag gtttgcgagg ccataaccga
    33481 ggcctttttc gcccattatg gcgagcgcgt ggaagcggaa atcatctccc cggacaaaac
    33541 gccagacttg ccaaacttcg ccgaaacctt tgcccgccag agtagctggg aatggaactt
    33601 cggtcaggct ccggcattct cgcatctgct ggatgaacgc tttacctggg gcggcgtgga
    33661 actgcatttc gacgttgaaa aaggccatat cacccgcgcc caggtgttta ccgacagcct
    33721 caaccccgcg ccgctggaag ccctcgccgg acgactgcaa ggctgcctgt accgcgcaga
    33781 tatgctgcaa caggagtgcg aagcgctgtt ggttgacttc ccggaacagg aaaaagagct
    33841 acgggagtta tcgacgtgga tagcgggggc ggtgcggtaa ctcgctgatt aaaatatcca
    33901 aggaaggaaa tatgatcgat ctggaaaacc aggaacggga gattatcaat ttaatgttca
    33961 gccagcgtat ttcctggcta gccgccgtgc gtatcaggca taaattatcg cttgctgaag
    34021 taagtaaaat gttaggtatc tcgattaatt ctctaaaaca gatcgaaaag acggaacggc
    34081 ttagctcaaa catcaaaagc aaaatggcag aaatttatgg ttgtccacca gagttgctga
    34141 t
//
LOCUS       DAZFQD010000038        33941 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.038, whole genome shotgun sequence.
ACCESSION   DAZFQD010000038 DAZFQD010000000
VERSION     DAZFQD010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33941
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.038"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(42..491)
                     /gene="rplI"
                     /locus_tag="ACHKTW_001924"
     CDS             complement(42..491)
                     /gene="rplI"
                     /locus_tag="ACHKTW_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFO6462956.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(533..760)
                     /gene="rpsR"
                     /locus_tag="ACHKTW_001925"
     CDS             complement(533..760)
                     /gene="rpsR"
                     /locus_tag="ACHKTW_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFO6462957.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            complement(765..1079)
                     /gene="priB"
                     /locus_tag="ACHKTW_001926"
     CDS             complement(765..1079)
                     /gene="priB"
                     /locus_tag="ACHKTW_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030894 - replisome [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HFO6462958.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            complement(1086..1481)
                     /gene="rpsF"
                     /locus_tag="ACHKTW_001927"
     CDS             complement(1086..1481)
                     /gene="rpsF"
                     /locus_tag="ACHKTW_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFO6462959.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            1808..2083
                     /gene="yjfY"
                     /locus_tag="ACHKTW_001928"
     CDS             1808..2083
                     /gene="yjfY"
                     /locus_tag="ACHKTW_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YjfY"
                     /protein_id="HFO6462960.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            complement(2212..2898)
                     /gene="ulaF"
                     /locus_tag="ACHKTW_001929"
     CDS             complement(2212..2898)
                     /gene="ulaF"
                     /locus_tag="ACHKTW_001929"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HFO6462961.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGNAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIASFLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(2898..3752)
                     /gene="ulaE"
                     /locus_tag="ACHKTW_001930"
     CDS             complement(2898..3752)
                     /gene="ulaE"
                     /locus_tag="ACHKTW_001930"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HFO6462962.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLRLAKTLGFDFVEMSVDETD
                     ERLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGREIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVAKARDWVKARMA
                     KAGMVEAA"
     gene            complement(3762..4412)
                     /gene="ulaD"
                     /locus_tag="ACHKTW_001931"
     CDS             complement(3762..4412)
                     /gene="ulaD"
                     /locus_tag="ACHKTW_001931"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFO6462963.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            complement(4426..4890)
                     /gene="ulaC"
                     /locus_tag="ACHKTW_001932"
     CDS             complement(4426..4890)
                     /gene="ulaC"
                     /locus_tag="ACHKTW_001932"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HFO6462964.1"
                     /translation="MKLRDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            complement(4900..5205)
                     /gene="ulaB"
                     /locus_tag="ACHKTW_001933"
     CDS             complement(4900..5205)
                     /gene="ulaB"
                     /locus_tag="ACHKTW_001933"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HFO6462965.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKAHFPQDVK"
     gene            complement(5221..6618)
                     /gene="ulaA"
                     /locus_tag="ACHKTW_001934"
     CDS             complement(5221..6618)
                     /gene="ulaA"
                     /locus_tag="ACHKTW_001934"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFO6462966.1"
                     /translation="MEILYNIFTVFFNQVMTNAPLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDTYASMMATIDRMGDAYS
                     WVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGLIAVTLFIFGYSMWTTII
                     CTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASWIAYKVAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVNWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACGSSILIIPGFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWAVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMAVIIVIALAYMFFAGRAL
                     RAEEDAEKQLAEQSA"
     gene            6973..8037
                     /gene="ulaG"
                     /locus_tag="ACHKTW_001935"
     CDS             6973..8037
                     /gene="ulaG"
                     /locus_tag="ACHKTW_001935"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HFO6462967.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEMKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            8145..8900
                     /gene="ulaR"
                     /locus_tag="ACHKTW_001936"
     CDS             8145..8900
                     /gene="ulaR"
                     /locus_tag="ACHKTW_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HFO6462968.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLD
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(8897..9646)
                     /gene="yjfP"
                     /locus_tag="ACHKTW_001937"
     CDS             complement(8897..9646)
                     /gene="yjfP"
                     /locus_tag="ACHKTW_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFO6462969.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDAARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLDDRLAVGGASMGAMTALGITARHPTVRCTASMMGSGYFTSLARSLFPPLIPETTA
                     QQNEFNNIVAPLAEWEATNHLEQLSDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            9828..10157
                     /gene="bsmA"
                     /locus_tag="ACHKTW_001938"
     CDS             9828..10157
                     /gene="bsmA"
                     /locus_tag="ACHKTW_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HFO6462970.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            10306..10581
                     /gene="yjfN"
                     /locus_tag="ACHKTW_001939"
     CDS             10306..10581
                     /gene="yjfN"
                     /locus_tag="ACHKTW_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protease activator YjfN"
                     /protein_id="HFO6462971.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(10698..12323)
                     /gene="aidB"
                     /locus_tag="ACHKTW_001940"
     CDS             complement(10698..12323)
                     /gene="aidB"
                     /locus_tag="ACHKTW_001940"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HFO6462972.1"
                     /translation="MHWQTHTVFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQVLCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPITMTFAATPLLLQMLPAPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTTGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEGSGNIMCLDVLRVLNKQAGVYDLLS
                     EAFVEVKGQDRYFDRAVRRLQQQLRKPAEELRREITHQLFLLGCGAQMLKYASPPMAQ
                     AWCQVMLDTRGGVRLSEQIQNDLLLRATGGVCV"
     gene            complement(12407..13570)
                     /gene="yjfC"
                     /locus_tag="ACHKTW_001941"
     CDS             complement(12407..13570)
                     /gene="yjfC"
                     /locus_tag="ACHKTW_001941"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HFO6462973.1"
                     /translation="MLRHNVPVRRDLDQIAANNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSREPFYFCCCQDTDEDRATVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLCKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKSQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            complement(13573..14211)
                     /gene="yjfM"
                     /locus_tag="ACHKTW_001942"
     CDS             complement(13573..14211)
                     /gene="yjfM"
                     /locus_tag="ACHKTW_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HFO6462974.1"
                     /translation="MARKRKSRNNSKIGHGAISRIGRPHNPFEPRRNRYAQKYLTLVL
                     MGGAVFLIVKGWSESGDVDNDGDGTFYSTVQDCIDDGNNADICARGWNNAKAAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASR
                     PVWRNTSGDYSWRSGSGKKESYSSGGFTTKKASTVSRGGYGRSSSARGHWGG"
     gene            complement(14221..14619)
                     /gene="yjfL"
                     /locus_tag="ACHKTW_001943"
     CDS             complement(14221..14619)
                     /gene="yjfL"
                     /locus_tag="ACHKTW_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HFO6462975.1"
                     /translation="MHILDSLLAFSAYFFIGVAMVIIFLFIYSKITPHNEWQLIKNNN
                     TAASLAFSGTLLGYVIPLSSAAINAVSIPDYFAWGGIALVIQLLVFAGVRLYMPALSE
                     KIINHNTAAGMFMGTAALAGGIFNAACMTW"
     gene            complement(14637..15296)
                     /gene="yjfK"
                     /locus_tag="ACHKTW_001944"
     CDS             complement(14637..15296)
                     /gene="yjfK"
                     /locus_tag="ACHKTW_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjfK family protein"
                     /protein_id="HFO6462976.1"
                     /translation="MSGFFQRLFGKDNKPAIARGPLGLHLNSGFTLDTLAFRLLEDEL
                     LIALPGEEFTVAAVSHIDLGGGSQIFRYYTSGDEFLQINTTGGEDIDDIDDIKLFVYE
                     ESYGISKESHWREAINAKVMGAMILNWQEKRWQRFFNSEEPGNIEPVYMLEKVENQNH
                     AKWEVHNFTMGYQRQVTEDTYEYLLLNGEESFNDLGEPEWLFSRALGVDIPLTSLHII
                     G"
     gene            complement(15347..16045)
                     /gene="yjfJ"
                     /locus_tag="ACHKTW_001945"
     CDS             complement(15347..16045)
                     /gene="yjfJ"
                     /locus_tag="ACHKTW_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HFO6462977.1"
                     /translation="MGILKSLFTLGKSFISQAEESIEETQGVRMLEQHIRDAKAELDK
                     AGKSRVDLLARVKLSHDKLKDLRERKASLEARALEALSKNVNPSLINEVAEEIARLEN
                     LITAEEQVLSNLEVSRDGVEKAVTATAQRIAQFEQQMEVVKATEAMQRAQQAVTTSTV
                     GASSSVSTAAESLKRLQTRQAERQARLDAAAQLEKVADGRDLDEKLAEAGIGGSNKSS
                     AQDVLARLQRQQGE"
     gene            complement(16064..16465)
                     /gene="yjfI"
                     /locus_tag="ACHKTW_001946"
     CDS             complement(16064..16465)
                     /gene="yjfI"
                     /locus_tag="ACHKTW_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2170 family protein"
                     /protein_id="HFO6462978.1"
                     /translation="MTWNPLALATALQTVPEQNIDVTNSESALIIKMNDYGDLQINIL
                     FTSRQMIIETFICPVSSISNPDEFNTFLLRNQKMMPLSSVGISSVQQEEYYIVFGALS
                     LKSSLEDILLEITSLVDNALDLAEITEEYSH"
     gene            complement(16592..17323)
                     /gene="rlmB"
                     /locus_tag="ACHKTW_001947"
     CDS             complement(16592..17323)
                     /gene="rlmB"
                     /locus_tag="ACHKTW_001947"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFO6462979.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLVLVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(17503..19944)
                     /gene="rnr"
                     /locus_tag="ACHKTW_001948"
     CDS             complement(17503..19944)
                     /gene="rnr"
                     /locus_tag="ACHKTW_001948"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFO6462980.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(19983..20408)
                     /gene="nsrR"
                     /locus_tag="ACHKTW_001949"
     CDS             complement(19983..20408)
                     /gene="nsrR"
                     /locus_tag="ACHKTW_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HFO6462981.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKSASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(20613..21911)
                     /gene="purA"
                     /locus_tag="ACHKTW_001950"
     CDS             complement(20613..21911)
                     /gene="purA"
                     /locus_tag="ACHKTW_001950"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFO6462982.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPTELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(22015..22212)
                     /gene="yjeT"
                     /locus_tag="ACHKTW_001951"
     CDS             complement(22015..22212)
                     /gene="yjeT"
                     /locus_tag="ACHKTW_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HFO6462983.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(22294..23298)
                     /gene="hflC"
                     /locus_tag="ACHKTW_001952"
     CDS             complement(22294..23298)
                     /gene="hflC"
                     /locus_tag="ACHKTW_001952"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HFO6462984.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(23301..24560)
                     /gene="hflK"
                     /locus_tag="ACHKTW_001953"
     CDS             complement(23301..24560)
                     /gene="hflK"
                     /locus_tag="ACHKTW_001953"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HFO6462985.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(24646..25926)
                     /gene="hflX"
                     /locus_tag="ACHKTW_001954"
     CDS             complement(24646..25926)
                     /gene="hflX"
                     /locus_tag="ACHKTW_001954"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome rescue GTPase HflX"
                     /protein_id="HFO6462986.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKATGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(26002..26310)
                     /gene="hfq"
                     /locus_tag="ACHKTW_001955"
     CDS             complement(26002..26310)
                     /gene="hfq"
                     /locus_tag="ACHKTW_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HFO6462987.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(26396..27346)
                     /gene="miaA"
                     /locus_tag="ACHKTW_001956"
     CDS             complement(26396..27346)
                     /gene="miaA"
                     /locus_tag="ACHKTW_001956"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFO6462988.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(27339..29186)
                     /gene="mutL"
                     /locus_tag="ACHKTW_001957"
     CDS             complement(27339..29186)
                     /gene="mutL"
                     /locus_tag="ACHKTW_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFO6462989.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMNVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFNHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRSIPENRVAAGRNHFAEPAAREPVAPRYTPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKPKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLSLPVAERWLRQAQLTPGEAPVCAQPLLIPLRLKVSAEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(29196..30533)
                     /gene="amiB"
                     /locus_tag="ACHKTW_001958"
     CDS             complement(29196..30533)
                     /gene="amiB"
                     /locus_tag="ACHKTW_001958"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HFO6462990.1"
                     /translation="MMYRIRNWLVATLLLLCTPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADAPPPPPPPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVARKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQHEKQSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(30552..31013)
                     /gene="tsaE"
                     /locus_tag="ACHKTW_001959"
     CDS             complement(30552..31013)
                     /gene="tsaE"
                     /locus_tag="ACHKTW_001959"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFO6462991.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(30985..32532)
                     /gene="nnr"
                     /locus_tag="ACHKTW_001960"
     CDS             complement(30985..32532)
                     /gene="nnr"
                     /locus_tag="ACHKTW_001960"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HFO6462992.1"
                     /translation="MTDHTMKKNPVSIPHTVWHADDIRRGEREAADALGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLATAVGIEVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANSH
                     PAPIAAVDIPSGLLAETGATPGAVINADHTITFIALKPGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPGEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            32531..33670
                     /gene="queG"
                     /locus_tag="ACHKTW_001961"
     CDS             32531..33670
                     /gene="queG"
                     /locus_tag="ACHKTW_001961"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFO6462993.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGAIPEELRPLIGNRIYGCDDCQLICPWNRYSQLTTEEDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILTALESRKGEH
                     PLLDEHIAWAIAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(33653..33706)
                     /locus_tag="ACHKTW_001962"
     CDS             complement(33653..33706)
                     /locus_tag="ACHKTW_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6462994.1"
                     /translation="MRFYFYSQAVDESGVTR"
BASE COUNT         8106 a         9401 c         8350 g         8084 t
ORIGIN      
        1 accaatggtc ggcgtttttt tacgtctcgt tgaataacga attattcagc tactacgttt
       61 acgatcactt tcgcgaatac ttcgctgtga acctggaagc tcacttcgtg ttcgccagtg
      121 gtacgcagaa cgccgttcgg cagacgaact tcgctcttag ccacttcaac gccagctgca
      181 gttacagcgt cagcgatgtc gcgagtaccg atggaaccga acagtttacc ttcgtcgcca
      241 gctttagacg cgatggtaac agtttccagt gcattgattt tctcagcgcg agcattagca
      301 gctgccagaa cttcagccag tttagcttcc agttcagcgc gacgtgcttc gaagaattca
      361 atgtttttct tggtagctgg aacagcttta ccctgcggta ccaggaagtt acgagcatag
      421 cccgctttaa cgtttacctg atcacccagg ctacccaggt ttgctacttt atcaagcaga
      481 ataacttgca ttaccttatc ctctcaaagt cgtattaatg gaccgtgacc gattactgat
      541 ggcgatcagt gtacggcagc agggacaggt agcgagcgcg tttgatagcg cgagccagct
      601 gacgctggta ttttgcacgg gtaccggtga tacggcttgg gacaatctta ccgctttcgg
      661 tgatgtagtt tttcagcgta gcgatatctt tatagtcgat ctcttgaacg ccttccgcgg
      721 tgaaacggca gaacttgcga cgacggaaat aacgtgccat atggctagtc tccagaatct
      781 atcaattcaa tctgctcggc atgcaaaacc attttgctca gtccgttctt tgccttgtgg
      841 catgaaatga acccctgaac ggttatgcga ctgccgaccg ttatactgtg agtaatggcc
      901 tggttttcgt gtccgctaac aataacgggc atttgacacc atgcctgccg gtgaaagccg
      961 gcttcctcct gcacagaacg atgctcaagc acgaactggc agtgaggaat tcctgatgga
     1021 ctgacctttc gaaggggagt cctgcacacg gtgccggaca acaccagacg gttggtcatc
     1081 agaaattact cttcagaatc cccagcttca gcatcatcag cggtttcgtt tgcgaaatca
     1141 tcgcgacgct cacggcgctc gtctttcgct ttaaccatcg gagatgcttc ggtaacagcg
     1201 tgcttggtac gcataaccat gctgcggata acggcatcgt tgaagcggaa ggtagtttcc
     1261 agctcatcga tcacttcctg cggagcttca acgttcatca gaacgtagtg tgctttgtgc
     1321 agtttgttga tcgggtaagc cagctgacgg cggccccagt cttccagacg gtggatcttg
     1381 ccttctgcac cagtgatggc agcagtgtag cgctcgatca tgcccggaac ctgttcgctc
     1441 tggtcaggat ggaccataaa aacgatttcg taatgacgca tcgaattgct ccttacggat
     1501 tattcagcct cctgtctggg tcagccgaat cccggggagg caaggaacgt gttaaaggtc
     1561 ggctgaaaaa tgacgcgtta tagtacttgt atgccccgct aaactcaagg ccagccgaca
     1621 aataaatctc atctttcgct caaaagaact aactccctga tttgaagttc atcacattcc
     1681 gtcacagtgt tttttcgaac aactgcatca gaaatggtca ccatcaactt ctttatcatt
     1741 gattaaaatt aaatcagaag atcatcacag aggagagcac acaaagtcag ccgtcgagga
     1801 ggtatctatg ttcagtcgtg ttttagccct tctggctgtg cttttgctaa gtgcaaatac
     1861 atgggcagcc attgaaatca ataaccacca ggccagaaat atggacgatg tgcaaagctt
     1921 aggcgtgatt tatatcaatc ataatttcgc cactgaaagt gaagcacgtc aggcattaaa
     1981 tgaagaaaca gatgcgcagg gcgcaacgta ctaccacgta attctgatgc gggaaccggg
     2041 gagtaacggc aatatgcacg ccagcgcgga tatttatcgc tagcaccagg tataaccaac
     2101 gaaacattgc catagtttgc tttgccccct tcgcaggggg ctttttttga aaaacggctg
     2161 cggaattaga ccagttatct cccgaagaag gaaatttccg cagcgcgtgt tttacttctg
     2221 cccgtaataa gcgttaggac catgcttacg catgaagtgt ttattcatca ggaagctggc
     2281 gatgtgattg agttgtggat taatactgcg cgcaatccac gccattttcg ccacttcttc
     2341 catcactacc gcgttatgca ccgcgtcgtg ggcatctttc ccccaggcga acggtccgtg
     2401 ctgatacacc acaattcccg gcgtatgcag cggctcggcg ttgcccagcg tttcgataat
     2461 cactttgcca gtgttcagtt catactcacc ctgcacctct tcttcgctta atccacgcgt
     2521 acacgggatg tcgccaaaga agtagtcggc atgcgtagtg cctaacgccg ggatcgccag
     2581 ccccgcctgc gcccatgcgg tagcatgagt ggagtgggta tggacaatgc cgccaagcga
     2641 cggataacga cggtagagtt caagatgcgt cgcggtgtcg gaagacggac gatattcccc
     2701 ttccaccacc ttaccgctca tatcgaccac caccatatcg tctgctttca tggtttcgta
     2761 ggcgacgccg ctgggcttga tcaccaccag cccgcgttcg cggtcgatag cgctgacgtt
     2821 gccccaggta aaggtcacca gcccatagcg cggcagatcc atgttggctt caaatacctg
     2881 ctgttttagc ttttgcatta tgccgcctcc accatgcccg ctttcgccat gcgcgctttc
     2941 acccaatcac gcgctttcgc cacttctgcc gccgggtctt ccgccgtttc gctccacatc
     3001 tcaatcaggt acggcccgca atagccactc tgtttgagcg tttcgaaaca acgttcgaaa
     3061 tccactacac cttcgccaaa cggcacgttt ttgaagacgc caggtttggt gtctttcaca
     3121 tgtaccgcga cgatatgccc gattccggcc tgcaactcca tctgcacatc gttgtcccac
     3181 gccgacaggt tgccgatatc cgggtagagc tggaaccacg ggttgttgag atagtgcgcg
     3241 taacccagcg ccttgctgat ggagttcatc aacggataat ccatgatctc catcgccagc
     3301 gtcacctgcg cgcggctcgc catctcaacg ctctctttca ggccgtcacg gaaacgacga
     3361 cgcgtttcgt tattggcttc ctgatagtaa acgtcatagc ccgccagctg gatcacgcga
     3421 atgccgacgt cctgggcgaa ctggatagct ttacgcataa tctcccgccc ctgcgcccgc
     3481 accgcgtcat cttcactgcc cagcgggaaa cgacgatgag cagaaaggca catggacggc
     3541 acgcgcacgc cggtttcaac aatcgcattc accagcgcca gacgctgctc gcggctccag
     3601 tcgaggcgcg acaggcgctc gtcagtttca tctaccgaca tctcgacaaa atcgaagccc
     3661 aacgtttttg ccagtcgcag gcgttccagc cagcactccc cggcggggag cgctttttca
     3721 tagatgccaa gcgggatttg tttggacaac atatccgctc cttagcccca cagttcggcg
     3781 atggaacgtt tgaactgacg cgcggcttca accggagaag cggcatcacg gatgctgcga
     3841 ccggcgataa acacatgaat cggaatacct ttgaacagcg gcagatcttc cagcgccagg
     3901 ccaccggtga cggtgacttt gaagcccata tcggaaagac gtttgatcgc ggtaatgtcc
     3961 gcttcgcccc acgctacgcc tgcggcctgc gcgtcacggc tgcggtgata aaccacctgc
     4021 tgaatgcctg cgtcacgcca ctgctgcgcc tgttcccagg tccagtagcc ggtcaattcg
     4081 atctgcacgt cgccgttaaa ctcttttgct acgtccagcg cgcctttggc ggtgttgata
     4141 tccgcacagc aaattaccgt cacccagtca gcgttggctt cgaagcacat acgcgaaagg
     4201 attttgcccg catcagcaat tttagcgtct gccagtacga ttttgtgcgg gtagagcgct
     4261 ttcaggtcac gaaccgcacg cacgccttcg cccacgcaca gaatggtacc cacttcgata
     4321 atgtcgactt cttcggcaat caggcgagtg gtttcgtagg cgctatccat agtctggttg
     4381 tccagcgcga cttgcaacat cggtaatgac atcttcaatt ccttcttaag ctgccgcgtt
     4441 ggtgcggtca atgagatcca gtacttcctg ctcggtacgg caggcacgta aacggtcgaa
     4501 attttcttca tcttcaaaca ggttgacgat ctgcatgatg cccacttcct ggtgcgtgtt
     4561 ggcatcgacc gccgccatgg tgatgaggat atccaccggg tcgttatctt cgtggttgaa
     4621 ctccagcggc tttttcagcg tcaccagcgc aaaaccggtt ttcttaacgc cctcttccgg
     4681 gcgaccgtgc ggcattgcca ggcctggtgc gagcacaaag tacggaccgt gctgttcaac
     4741 cgcatccaga atcgcctggt agtaacgcgg ctcgacaaca tccgccgcca ccagcaggtc
     4801 aacgccgatt tttactgcgt cctgccatgt ctcagcttca gcctgcaggc ggatggattt
     4861 attctccgcc agcgaatcac gtaatttcat ggcgcgtcct tacttcacat cctgcgggaa
     4921 atgcgctttg atcacttcca gcagtttcgg gccaaagtcg gcaggagaga gcatgttgcg
     4981 cacgccaacc acgtatttgt tgccggtcac ggtgatttcg cccgcaatgt gcgtagaagc
     5041 gatgatgata tccgcgccgc tcaactcgct tttgtattcg ccaaccgcgc agctatttac
     5101 cgtgtggtca atgtttgatt gggttaaaaa ctggtccact ttcatcttca tgatcatgga
     5161 actgccttgt ccgttgccac acacagccag aatacgtacg gtcataatca aaactcctta
     5221 ttaagcagac tgttctgcca gttgtttttc tgcatcttct tctgcgcgca gtgcgcggcc
     5281 agcgaagaac atataagcca gtgcaattac aatgatgacg gccataaagg cgataccgat
     5341 ggagaagaag ccctgcatca tcggcggtgc cagaatcgac cagtccgcca tgcccatcca
     5401 ggcgctcata ccggtgagtt tcaccgccca gacgcagcca aagatttcaa tcatccccat
     5461 caccagacat atcttcagcg ccgcacgcca gccgccgaag tggttagcga acacgccgat
     5521 ggtggcgttg gagaagaaca tcgggataaa gccgggaata atcaggatcg aggaaccgca
     5581 ggcgaccagg atgccaaccg caatcagctg accgatggtg ccccacataa agccccaaac
     5641 cacggcgttc ggcgcgaagc tatagatagc cgcacagtca atcgccagaa ccgcaccagg
     5701 gatcaggcgc tgagagatac cgttaaatgc ttcagagagt tccgccacaa acatgcgcac
     5761 gccctgagtg atgatgaaga tcgccaccgc gaaggagaaa ccagtttgca ggatgtacac
     5821 cgtccagttc actttgcctg ccatcgcctg cacggtgtcg ataccgaagg agagcagaat
     5881 ggcaccaaag aagatggtca tcacaatcgc cgtggagacg atgttgtcgt ggaaaatatt
     5941 cagccagcct ggcagtttga ggtcttcaac gctttcctct tttttgccga ggaacggcgc
     6001 aactttatag gcaatccatg atgcaaactg ttgctggtga ccgatggaga aaccgcagcc
     6061 gtccgtcact tcctgagtcg gcttgtacat catgttggag gtaatgcccc agtagagcga
     6121 aaccagaatc gccgtgcaga taatggttgt ccacatggag tagccgaaaa taaacaacgt
     6181 aacggcaatc agccccgcct gctggaacat gatatgacca gtcagcatga tggtacgaat
     6241 accggtaatg cgacgcagca gcacgtaaca gatgttcagc gccagcgcta acaacaccgc
     6301 gtaacctacc cagctatagg catcgcccat gcggtcgatg gttgccatca tcgacgcgta
     6361 agtatcggaa attgcgccgt taatgccgta gacttcggac attttcgcca ccaccggttt
     6421 gaaggtgctg gtgaggatgc cggaccctgc ctgcaacaac atgaaaccaa ttatggtttt
     6481 aatcgtgcct ttaataataa cgctgacact tttgcgcagt aggatgtagc ccagacaggt
     6541 cacaataccc agcaacaacg gggcattggt catgacctgg ttaaaaaaca cggtaaagat
     6601 gttgtagagg atctccataa cgatctcctg aagaagaggt aaccgggtat tccacacacc
     6661 cggaatgttg tgcactcact ctaattttca aaagtaatca caacaagatt atttatgatt
     6721 aaatgtgacg cgacccgcaa ataattccac atggatagaa tggtattcac acaacgaaac
     6781 aactattcat ttaaattcat ttagttaatt gaattacaca ttccattaaa tctttccaca
     6841 ctcaaaatca acaaccactt ccctggcaaa aagtcaaaaa atggtcattg ccttcctgaa
     6901 aaaatagtgt caggattaat caaaaccaat cacacattga ttcgaattga acaatcatga
     6961 aggtaaatgg cgatgagtaa agtgaaaagt atcacccgtg aatcctggat cctgagcact
     7021 ttcccggagt ggggtagctg gttgaatgaa gaaattgaac aagaacaggt cgctccaggc
     7081 acatttgcga tgtggtggct tggctgcacc gggatctggt tgaaatcgga aggtggcacc
     7141 aacgtttgcg ttgatttctg gtgcggcact ggcaaacaaa gtcacggtaa cccgttaatg
     7201 aaacagggtc accagatgca gcgcatggct ggcgtgaaaa aactgcagcc aaacctgcgc
     7261 accaccccgt ttgttcttga tccgtttgcg attcgccaga tcgacgcggt actggcgact
     7321 cacgatcaca acgatcatat cgacgttaac gttgctgctg ccgtgatgca gaactgtgct
     7381 gatgacgtac cgttcattgg accgaaaacc tgtgtggatt tgtggattgg ctggggcgta
     7441 ccgaaagagc gttgcatcgt ggtcaaaccg ggcgatgtag taaaagtgaa agacattgaa
     7501 attcatgcgc ttgacgcttt cgaccgtact gcactgatca ccctgcctgc cgatcaaaaa
     7561 gcggctggcg tactgccaga tggcatggac gatcgcgcag taaactacct gttcaaaacc
     7621 cctggcggtt ccctatatca cagtggcgat tcccactact ctaactatta tgcgaagcac
     7681 ggtaacgaac atcagatcga tgtggcgtta ggatcgtatg gtgagaaccc gcgcggtatt
     7741 accgacaaaa tgaccagcgc cgatatgctg cgtatgggtg aagccctgaa tgcgaaagtg
     7801 gttatcccgt tccaccacga catctggtca aacttccagg ccgatccgca agagatccgc
     7861 gtgctgtggg agatgaaaaa agatcgtctg aagtatggct tcaagccgtt tatctggcag
     7921 gttggcggca aatttacctg gccgctggat aaagacaact tcgagtacca ctatccgcgc
     7981 ggtttcgatg attgcttcac tattgaaccg gatctgccgt tcaagtcatt cctgtaatct
     8041 gtcgtttata ccggatagcg tggagaagtt cgcgctatcc ggcgtagtat ttcctggcta
     8101 tttcaaatat catctaaaaa aatcaaattt tatcggaata gctcatgact gaagcacaaa
     8161 gacatcaaat cctcctggaa atgctcgcac aattgggctt tgtgaccgtt gagaaagtcg
     8221 ttgagcgtct gggaatttcg cctgccactg cgcgacgcga tatcaataaa cttgacgaaa
     8281 gcggcaaact gaaaaaagtg cgcaatggcg cagaagctat tacccaacag cgcccgcgct
     8341 ggacgccgat gaatctgcat caggcgcaga atcacgatga aaaagtacgt atcgctaaag
     8401 cggcctcgca gctggttaat ccgggcgaaa gcgtagtcat caactgcggc tccaccgcgt
     8461 ttctgcttgg gcgggaaatg tgtggcaagc cagtgcaaat catcactaat tatctaccgc
     8521 tggcaaatta cctgatcgat caagaacatg acagcgtgat cattatgggc ggacagtaca
     8581 acaaaagtca gtccatcact ttaagcccgc agggcagcga aaacagtctc tatgccgggc
     8641 actggatgtt taccagcggc aaaggactga cagcagaagg gttgtataaa accgatatgc
     8701 tgacggcaat ggcagagcag aagatgctga gcgtggtagg gaaactggtg gtactggttg
     8761 atagcagtaa gattggcgaa cgcgcgggaa tgctttttag ccgtgccgat caaattgata
     8821 tgctgatcac cggcaaaaat gctaacccgg aaatcctgca acaactggaa gcgcaagggg
     8881 tcagcattct gcgtgtttaa agatgctggc ggaaaaatgt cactgcagca tctaacgcct
     8941 caggcgtaat gcggtgacgc acgcctggct gccatgaaca ggttagcagt ttatcccgtc
     9001 ccgtctcgct taaggcctgc tgcaaacgta gtgattcgtc ggcaggcaca acgtcatcgt
     9061 cgaggccatg ccacagcagt agaggtctgt cactaagttg ttccaggtgg tttgtcgctt
     9121 cccactctgc cagcggcgcg acaatgttat tgaattcatt ctgctgtgct gtcgtttcag
     9181 gtatcagcgg tggaaacagt gaacgggcga gtgatgtaaa atagcccgat cccatcatgc
     9241 tggcggtaca tctcaccgtg gggtggcgag cggtaatccc cagtgccgtc atcgcgccca
     9301 tcgacgcacc accgactgcc agacggtcat caagcagcca gttttcttcg gctattgccg
     9361 cacgtaaagt agtgaattcc tgcatacttt gtagcaagat ttgccagaat tgatttaacc
     9421 gccgcgctgc gtcaccacta aaacggctac cgtgatcggg cgcatccggc atgatcactc
     9481 gcaaaccagc ctgcgccagc gcaacggcaa aatagctata caccagactg gatgaagtaa
     9541 agccgtgata aaaaattacg cacggtaacg gggtatcttt ttgcccgaca ggataagcat
     9601 gaagaacggg aatatctgcc agctcgcgtg attctatttc aatcatctgt ctctcctttt
     9661 tgtctggtat gacatgaaga aatccttagc acaagtttct gctggcggta ttagttcata
     9721 atgttgagat gtgggttacg ctttcgtcac gttttcattc gaaaatcgcg taccaggtaa
     9781 caattcggga acattccccc aaagttaaat taataaggca ctacactatg gttagcagga
     9841 aacgtaatag cgttatttac cggtttgcca gtttattatt ggtgttgatg ttaagtgcct
     9901 gtagcgcact gcaaggtacg ccacagccag caccgccagt aacggaccat ccgcaagaga
     9961 ttcgccgcga ccagacgcaa gggttacagc gaataggcag cgtaagcacg atggttcggg
    10021 gttctccgga tgacgcatta gcagaaatca gagcgaaagc tgtcgctgca aaagctgatt
    10081 attacgttgt cgtcatggtg gacgaaacca tcgtgacagg acagtggtat tcacaagcca
    10141 ttttgtaccg taaataaatc agacaactta catagtcagc tttacattgc tttgcgtccc
    10201 tatgcgttta cctgtgcaca ataaattacc ggctggaagc ctgggcgttt attaccgcga
    10261 tggaatgccc tgcaacatgt ggggaaacga aaaatggagc tgacgatgaa acaattactt
    10321 gcctcaccct cgctgcaatt agtgacttat cctgcgagcg ccacggcaca gtctgcagaa
    10381 ttcgccagtg ctgactgcgt gacgggcctg aatgaaatag gtcagatctc agttagcaat
    10441 atttcagggg atccgcagga cgtggaacgt atcgtggcgt taaaggccga tgaacaaggt
    10501 gcgtcatggt accgcattat taccatgtac gaagaccagc agcctgataa ctggcgcgta
    10561 caggcgatac tgtatgcatg agtgtcatct acagcaaatg aacaatgccg gatgcggcgc
    10621 gagcgccttg tccggcctac aattttcaca tatttcattt agttaatcga aaccagcgtc
    10681 gcatcagtcg tatacgctta cacacacact ccccccgtcg cccgcagcaa taaatcattc
    10741 tggatctgct ctgacaaccg tacgccgccg cgcgtatcta acatcacctg acaccacgcc
    10801 tgcgccattg gcggagaagc atatttcaac atttgcgcac cgcagcccag caggaatagc
    10861 tgatgagtaa tctctcgcct cagttcttca gctggcttac gcagctgctg ctgtaaacga
    10921 cgaaccgcgc gatcaaaata gcgatcctgc cctttcactt ccacaaacgc ttccgacaat
    10981 aagtcgtata cgcccgcttg cttattgaga acgcgcagca catccaggca cataatattg
    11041 ccggaacctt cccaaatact gtttaccggc atctcccggt aaagccgcgg cagctcgctc
    11101 tcctcgcaat aaccaatgcc gcccagcacc tccatcgctt cggccacaaa cggcatacct
    11161 cttttgcaga tcacaaattt tgccgcaggc gtaaataaac gcgcccacag ggcttctttg
    11221 gcatcggcac gccggtccca cgctcgcgca agacgaaaca gcaacgccgt ttgcccttca
    11281 agctgaagtg ccatgcgact taagacatga cgcataaggg gctgttggat caatggatta
    11341 ccaaaaacat ggcgttgatg tgcatgataa atcgccagcg aaaatgcacg gcgcatcatg
    11401 gcatggctac ccagggcgca atcaaaacgc gtcatcccac ccattttcag gatcagacga
    11461 attccttccc cttcctgccc caacaaccag ccaatggcat cctgaaactc cacttcgcag
    11521 ctggcgttag agcgattacc cagcttatct ttcagccgct cgaggcgaat cgcgttgcgt
    11581 tgcccgtcag gcaaaaagcg cggcacaaaa aagcaggaca aaccgcccgt agtctgcgcc
    11641 agcaccagat gcgcatcgct ttgcggcacc gagaaaaacc atttatgccc caccagccga
    11701 taagagccat cttccaggcg ctctgcgcgg gtggtgttgc tcatgacatc ggaaccgccc
    11761 tgcttttccg tcattcccat gccaatcaac aaaccgcgtt tttgcccacc tggcaataag
    11821 tgagaatcgt agcgatcgct cagcagcggc gtggtccagt cctgaaacgg cgcgggtaac
    11881 atctgtaaca gcaatggcgt ggcggcaaag gtcatggtta tcggacataa cgaccctgcc
    11941 tcaacctgtg cgtgcaacat aaaacgcgcc gcgcgcgcca caaatgcgcc ggagcgagcg
    12001 tcttcttccc aggcaagatt gtgcacccga ttggtacata gcacctgcat cagcaggtgc
    12061 caggcggggt gaaaacgcac atcgtccaga cggcgtcctt gcgcatcgta gcgcaataat
    12121 tcaggcggat tcacattcgc cagccgcccc agttcaaggg attcagccgt tcctaactgc
    12181 tgaccaatac tggctagaaa atcgctatcc cagccagcac cttcacgcgt taccgcttcg
    12241 cagagcgcgc catcagacag gtataagttg ctgttattta atggtatagg ttgattaaaa
    12301 acggtgtgag tttgccagtg cactgtgtct ccctccatca atggcagtca ccattagtat
    12361 ggtcactgcc atggattcat gacagaaaga gattgctaaa acattcttat ccagcaatgt
    12421 aatgcggaac gaaacgtgag gtgtctttgg tgatcagtgt gttatcttca cggatcccca
    12481 ttccgcacgc ttcatcatcg acaatccagc taccgatgag tgtgtagcta tcgccaaatc
    12541 gcggcagagg ttgaaacgcc tggtagatca tcggttcatc ggcgtaatca ccatcagcgt
    12601 ggtcaacgac gttattctga ccgtcgaaaa tggtgacgtt tccgccttcg cgcgagtaga
    12661 tcggtttacg cacatagctt tcgccagcgg caatctgcga tttttcaccc tcaaaccagg
    12721 atgcaagaag gttaggatga ccagggaaga agcgccaaag cagcggcatt agccctttat
    12781 tactcaagat acttttccat aacggctcca cccattgctc gcgacgcttg cacagcagcg
    12841 gaccgttatc gtcacgcatc atccactcca gcggatacag cttaaatgca cgctggatga
    12901 cattatcatc aagatcggtc agtacaccgc cgacgcccaa accgagatct tcaatgtaga
    12961 taaaccgcga ctcctgccct gcctgctggg cgcagtcctg caagtacagc acggtagccc
    13021 tgtcttcatc ggtgtcctga cagcagcaga aataaaacgg ctcccggcta taaagctcgc
    13081 taaagcgtga aatcaggcgt tcctgaatgg cattgtactg atcggcatca cgcggaataa
    13141 tgccgctgcg ccgggcatcc tccagccaca accactggaa ataagccgac tcatacaacg
    13201 aggtcggcgt atcggcgttg tactccagca gcttcaccgg cgcattgcca caccaggcaa
    13261 aatccatccg gccatacagc gaaggatcgc gtgcgcgcca gctttcggcg atcacatccc
    13321 agtacaacgg cgggatcgcc agttgcgtca ggatctcttc gtctttaacc gcgcgatcca
    13381 ccacctcaag gcacatctga tgcagttccg cagtcggttt ttcgatctgc tcttcaatct
    13441 ggcgcagagt aaaacggtaa gcccgactct cgtcccaata gatttcattg tcgatgatat
    13501 gaaagtcgaa accgttgtta gcggcgatct ggtccagatc ccgtcgcaca ggaacgttgt
    13561 gtctcagcat gattagcctc cccaatgccc acgggcgcta gaagaacgac catagccgcc
    13621 gcgcgaaacg gttgacgctt ttttggtggt aaagccgcct gaagagtaag actctttttt
    13681 gccagaaccg gagcgccagg agtaatcacc ggaagtgttg cgccagacgg ggcgggaagc
    13741 aaatgaggat ccgccgctgt tatagacgaa cggctcatcg cgatctttac gaataactcg
    13801 gcttaacaaa aatccagaaa cgaccgggat ccaactctgt tcaacattgt cgtagtagca
    13861 attttcgtac ttagactgac aattctgctg agtcatattc ttcggaacat cggcataaaa
    13921 tgccgctttg gcgttgttcc agccacgcgc gcaaatgtcg gcattattac cgtcatcaat
    13981 acaatcctgc actgtcgaat aaaacgttcc gtcgccgtcg ttatcgacat cgccactttc
    14041 actccatcct ttgactatta aaaaaacggc accgcccatt agcaccaacg ttaagtattt
    14101 ttgtgcgtag cgattgcggc gcggctcaaa aggattatgc ggtcttccaa tgcgactgat
    14161 cgctccgtga ccaattttac tgttatttct tgatttgcgt tttctggcca tgatcgtcca
    14221 ttaccatgtc atacaagctg cgttaaaaat accaccagcc agtgcggcgg ttcccataaa
    14281 cattcctgct gcggtattgt gattaataat tttttcgctt aatgcgggca tataaagtct
    14341 gacaccagca aaaacgagta actgaatcac cagtgcaatc ccgccccagg cgaaatagtc
    14401 tggaatactc accgcattga ttgccgcact ggataagggg atcacgtaac ccaacaatgt
    14461 accgctgaat gccagtgacg ccgcggtatt attgtttttg attaattgcc attcgttgtg
    14521 cggtgtaatt ttggagtaga taaacagaaa aataatcacc atagccacgc caataaaaaa
    14581 ataggcacta aaggcaagaa gtgaatccag tatatgcatt gtaaacgtcc ttgtaattaa
    14641 ccaataatat gaagtgatgt cagcgggata tcgacaccca acgcgcgcga aaataaccac
    14701 tctggctcgc cgagatcgtt aaaagattcc tcaccattta acagcaaata ttcgtaagta
    14761 tcttcggtta cttggcgttg gtagcccatg gtaaaattat ggacctccca tttggcatgg
    14821 ttttgatttt ctactttttc cagcatgtag acgggttcga tatttcccgg ttcttcgctg
    14881 ttaaaaaatc gctgccagcg tttttcctgc cagtttaaga tcattgcccc catcactttg
    14941 gcgttgatcg cctcgcgcca gtgactctct ttactgatac cgtagctctc ttcatagaca
    15001 aagagcttga tatcatcaat gtcatcaata tcttcgccgc cggtggtatt gatttgcaga
    15061 aattcatcgc ctgaagtgta gtagcggaaa atctgactac cgccgcccag atcgatgtgg
    15121 ctgacagcgg ctaccgtaaa ttcctcaccc ggcagcgcaa tcagcaattc atcttccagc
    15181 aaacgaaacg ctaacgtgtc gagcgtaaag ccactattga gatgaagtcc cagcggaccg
    15241 cgagcgatag caggcttatt atccttgcca aacagacgct ggaaaaaacc agacatattt
    15301 tatccttaaa atagttgcca gccttttagc gaggctggca aaaaaattac tcgccctgtt
    15361 ggcgttgcag tcttgctaat acatcctggg cgctactttt attgctaccg ccaattccgg
    15421 cttccgccag cttttcatca agatcgcgac cgtctgcgac tttttccaac tgtgcagcag
    15481 catccaggcg agcctgacgt tcggcctgac gcgtttgcag gcgttttaag gattctgccg
    15541 ctgtcgaaac gctggaagat gcgccaacgg tagaggttgt tacagcctgt tgtgcacgct
    15601 gcatggcttc agtggcttta acgacttcca tttgctgctc aaactgagcg atacgctgcg
    15661 ctgtcgctgt aactgctttt tccacgccat cacgggagac ttccagattc gacaacactt
    15721 gctcttcagc ggtaatgaga ttctcaaggc gcgcgatttc ttcagcaact tcgttaatca
    15781 acgacggatt aacgttcttg cttaacgctt ccagcgcacg ggcttccaga ctggctttgc
    15841 gctcacgtaa atctttcagc ttatcgtgac ttaatttcac ccgcgccagc agatcaacgc
    15901 gagattttcc ggctttatcg agttcagctt tagcgtcacg aatatgctgt tccagcatgc
    15961 gcacgccctg ggtttcttca atggattctt ccgcctggga gataaacgat ttccccagcg
    16021 taaataaact ttttaaaatt cccataaata cccgtccttg taattagtgt gaatattctt
    16081 ctgtaatttc agccagatcc aatgcgttat ctaccagcga ggttatctca agcaggatat
    16141 cttcaagaga agatttaagc gataacgccc cgaaaacaat gtaatactct tcctgttgca
    16201 cgctggagat tcctaccgat gacaacggca tcatcttctg atttcttaat aagaaggtat
    16261 taaattcatc tggattgctg atactactta ccggacaaat aaaggtttcg ataatcattt
    16321 ggcgggatgt aaaaagaata ttgatttgca aatcgccata atcattcatt ttaataatta
    16381 atgcgctctc gctatttgtc acatcaatat tttgttcagg tacagtttgc agcgccgtcg
    16441 ccagcgccaa cggattccat gtcatatatt attccatata gatttagttt aaatattaat
    16501 aaaatgaata tttgcagtac gtaattatct taccagctat agacaaaaaa aaaccatcca
    16561 aatctggatg gcttttcata attcagacga actagctgcg ctgacgaacc gcttcaaata
    16621 agcaaattcc ggttgcaacc gaaacgttca gggaagaaac acttcctgcc attgggatgc
    16681 tgatcaactc atcgcaatgt tcacgggtca ggcgacgcat accttcacct tccgcgccca
    16741 tcaccagcac caggcgtccg gtcattttgc tctgatagag tgtatgatcc gcctcgcctg
    16801 ccgtaccgac gatccagata ttctcttcct gcaacatacg catggtgcgc gcaaggttag
    16861 tcacccgaat cagtggaacg ctttctgccg cgccgcaggc tacttttttc gccgtagcgt
    16921 tgagctgtgc ggagcgatct ttcggcacaa tcaccgcgtg aacgccagca gcgtccgcgc
    16981 tacgcaggca cgcgccgagg ttgtgcggat cggttacacc gtcgaggatc agcaggaacg
    17041 gttgatcgag cgaagcgatc agatccggca gatcgttttc ctgatactga cgtcctggct
    17101 tcacgcgggc gataatgccc tgatgcacgg caccgtcgct tttctcgtcg agatattggc
    17161 ggtttgccaa ctggataacc acgccctgag actcaagggc gtgaatcagc ggtaacagac
    17221 gtttatcttc acggcctttt aaaataaaga cttcctgaaa acgttcaggg gcgcgctcca
    17281 gcagggcctg cactgcgtgg atgccgtaaa tcatttcgct cattaatgta ctcgttgttt
    17341 acgttttgcc tgatgcacta cgtttatccg gcctacatga tctctgcaat atattgaatt
    17401 tgcgtgatta tgtaggccgg ataaggcgtt cacgccgcat ccggcatgaa caaagcgcat
    17461 tttgtcagca atctaaccct cttcttttaa agagggtatt gatcactctg ccactttttt
    17521 cttcgccgca cgcttcgctt tggtcgctgc ggctattttc tgcgttttcg ccgatggctt
    17581 tttcgctttt ctcgcgtctt tcttcgccgc tttcggcttc gtttttttct caccgcggaa
    17641 ggcgctgtct ggctcaaagt ttaccttttt accgacctga cgacgcttgc cgcctttttt
    17701 gcctgcatcg ccttttttcg ctttctcgcg cgccgtttta ccgacgttgc gcggtgcgcg
    17761 ttcactggag atcagactaa agtcgatttt gcgctcgtcc atattaaccg cttcgactcg
    17821 aacttccacg cgatcgccca ggcgataagt ctggccgctg gattccccca tcaggcgttg
    17881 ccctacctgg tcaaagcgat agtagtcatt gtccagcgaa gagacatgga ccagaccatc
    17941 aatgaacaag tcgtccagac ggacgaagaa gccaaagcca gtgacgctgg aaattacgcc
    18001 tttaaagacg ttacctacct ggtcgagcat gaagtcacac ttcagccagt cagcgacatc
    18061 gcgcgttgct tcgtcggcac gacgttccgc catcgaacag tgctgaccca gttgcaacat
    18121 ctcttccatc gaataatggt agccgccggt ttcagtggtg ttgccctgat gcccctgctc
    18181 tttcgccagc agatacttaa tggcgcggtg cagcgtcagg tcaggataac gacgaatcgg
    18241 cgaagtaaag tgcgcatagg actgcaatgc caggccaaag tgaccacggt tttctggatc
    18301 gtaaatcgcc tgtttcatcg agcgcagcag catggtttgc agcatttctg catcaggacg
    18361 gtcggcaacc gactccagca gttccgcgta gtcacgcggt tccggcttgt taccacccgg
    18421 cagctccagc cccagctccg ccagcactga acggaaagag gtaatcgctt cggtgctcgg
    18481 cttgtcgtga atacggaaca gtgccggttc tttcgctttc tcaacgaaac gcgccgccga
    18541 gatattcgcc agaatcatgc actcttcaat taatttgtgc gcgtcgttac gctgggtctg
    18601 ttcgatacgt tcaatacggc gttcagcgtt gaaaatgaac ttcgcttctt cgctctcaaa
    18661 tgagatccca ccgcgttctt cacgggcttt atccagcact ttatagaggt tatgcaactc
    18721 ttcgagatgc ttaaccagcg gggcgtactg ctcgcgcaga tcctgatcgc cctgcagaat
    18781 atgccagact ttggtgtagg tcagacgcgc gtgagagctc atcaccgctt cgtagaattt
    18841 gtagcccgtc aggcggcctt tcgacgaaac cgtcatctcg cacaccatac acaggcggtc
    18901 tacctgcggg ttgagcgaac acaggccgtt agagagcact tccggcagca tcgggataac
    18961 ctgcgaaggg aagtacaccg acgtgccacg gttacgcgct tctctgtcca gcggcgttgg
    19021 cggacgcaca tagtagctga cgtcggcaat cgcgacccat aaacgccagc cgccgccgcg
    19081 ttttttctcg cagtaaactg catcgtcaaa gtcacgggcg tcttcgccat caatggtgac
    19141 cagcggtaaa tcgcgcagat caacacggcc cgcttttgct tcttccggca cttcttcttt
    19201 cagcccggca acctgttgct caacagcctg cggccagatg tacggaattt catgggtacg
    19261 cagagcgata tcaaccgcca tgccggtgcc catattgtcg cccagcactt cgacgatttt
    19321 acccaccgct ttggtgcggc gagtcggacg ctgagtcagt tcgactacga ccacaaagcc
    19381 catccgcgcg cccatgatct gatcgggcgg gattaagata tcgaagctca gacggctatc
    19441 gtcaggaacc acaaagccga cgcccgcttc agtaaagtag cgaccaacaa tctggctggt
    19501 ttttggcacc agtacgcgga caatacgcgc ttcacgacga cctttacggt cagcgcccag
    19561 cggctgcgcc agcacctgat cgccatgaat gcaggttttc atctgctcgc tggagagata
    19621 caaatcatct ttacgccctt caacccgcag aaagccgtag ccatcacggt ggccaataac
    19681 ggtacctttc accaggtcga ggcgttccgg cagcgcatag cactgacgac gagtgaagac
    19741 cagttgacca tcgcgctcca tcgcgcgcag gcggcgacgc aggccttcaa gctgctcttc
    19801 gccttcaatg tgcagttcta ccgccagctc atcacggctg gccggttttt cacgtttggt
    19861 taaatgttcg aggataaatt cccgactagg gatgggattc gcgtattttt cagcttcgcg
    19921 ttcctggaaa ggatcttgtg acatctcggt tcctccgttg tcatctctga tgaagatttt
    19981 cgtcactcca ccagcaataa tttataaagc ggttgattct cttcaaccaa atcggcaagc
    20041 gtgtagttat ccagttccgt aagaaaactt tgcacggcct tagaaagcgc ctgtttcaac
    20101 cgacaggcag gtgtaatgtg gcaaaactca ctgctgcaat tcaccagcga taagggctcc
    20161 agctcgcgca ccacatcacc aatacgtatc gcactcgccg atttacccag gcgaatgccg
    20221 ccattttttc cacgaacagc agtcacgtag ccggcacgac taagttgatt gattattttg
    20281 accatatgat tacgggagac gccgtagacg tcagtcactt cagaaatact ggtcatccgc
    20341 ccttctggca atgacgccat gtagatcagc gcacgtaatc cgtaatcagt gaaactcgtt
    20401 aactgcacat caacctcaaa agggaaatcg ggaaaaaata catttatatt gatgataaac
    20461 cagccacaag ctgtgtcgct aatttatttc agttcgggga aggaaaaaag cgaggattta
    20521 acactgtgtc ggatagcgta atggcttaac cgacacagca atgtatcaca ctgttagccc
    20581 ggcaggcaaa atatctgcca ggcgtaccag aattacgcgt cgaacggatc gcgcagaatc
    20641 atggtttcag tacgatccgg accggtagag atgatatcga tcggcacacc agtcagctct
    20701 tcaatacgct tgatgtagtt cagcgccgcc tgcggcagac cgctacgatc tttcacgccg
    20761 aaggtggatt cagaccagcc cggcatggtt tcgtaaatcg gctctacacc tttccagtcg
    20821 tcagctgcca gcggagtggt agtcacttcg cgaccatccg gcatacggta agccacgcag
    20881 agtttaacct ctttcaggcc atccagaacg tccagtttag tcaggcagaa gccagacagg
    20941 gagttcagct gtaccgcacg acgaacggca acggtgtcca gccagccggt acgacgacga
    21001 cgccccgtag ttgcgccgaa ttcgttaccc tgcttgcaga ggaactcgcc agtttcatca
    21061 aacagttcgg tcgggaacgg acctgcacct acacgagtgg agtaagcttt gaggataccc
    21121 agaacgtaat caacataacg cgggcccagg ccggaaccgg tcgccacgcc accagcagtg
    21181 gtgttggaag aagttacgta cggataagta ccgtggtcga tatccagcag cgtaccctgc
    21241 gcaccttcaa acatgacgaa atcgccacgc tgacgcgcct ggtcgagcag gtcagaaacg
    21301 tcaaccacca tagaagtcag gatgtcggca acagccatcg tatcatccag aactttctgg
    21361 taatcaaccg cttcagcttt gtagtagtta accaactgga agttgtgata ttccatcact
    21421 tctttcagtt tttcagcgaa ggtttctttg tcgaaaaggt cgccaacacg cagaccgcga
    21481 cgtgctactt tatcttcata agcaggcccg ataccacgac cggtggtgcc gatcgctttc
    21541 gcgccacgcg ctttctcacg cgcgttatcc agcgcaacgt gataatcaag gatcagcgga
    21601 catgcttcag acagcagcag acgctcacga acggggatgc cacggtcttc cagttctttc
    21661 atctctttca tcagcgcggc cggagacagc acaacaccgt taccgatgat gctggttaca
    21721 ttctcgcgga gaatacctga tggaataaga tggagaacgg ttttttcacc gttgattacg
    21781 agagtatggc ctgcgttgtg accgccctgg tagcgtacaa catatttagc ccgttcagtc
    21841 agaagatcga cgatcttacc tttaccttcg tcaccccatt gggtgcccag tacgacgacg
    21901 ttgttaccca tttttcaaaa tcaccgtttg cttaaaaatg gattctacca tcgctttttc
    21961 agagttacag cactttttgc actcaaaaat gacctcgatc agtctgtttt ttgttcagcc
    22021 aatcgttttc ctcaacatgt agtagaccac aacgcccgca accacaagtc caccgccaaa
    22081 acgacgtaaa atattatcgg gcaaattggt catcgcagag atcatcttct tccatgcctt
    22141 cgggtaaagc atcggcccta aaccttccag taccaaaacc agggcaagcg ccagccagat
    22201 tgtcgaattc attcattatc cttatagaaa aagaaaacca ccgacatccc tgaggatgcg
    22261 gtggctttat tgacctgtac cgcagtcgtt atattaacgc gttgcggaag tcggcgtctt
    22321 catgtagcgg aagaaatcgc tatccgggct catgaccatc acgtcctgat tgccagagaa
    22381 gctgttctca taagcacgca ggctacggat gaatgcgtag aagtccggat ctttactgaa
    22441 tgcatcagca aacagtttgg ctgcttcggc atcgccttca ccacgcatga tgcggccctg
    22501 acgctcagct tctgccagcg ttctggtcac ttcatagtcg gcagtcgcgc gcagtttttc
    22561 cgcttcttcc tgaccttgtg aacggtgacg acgcgctacc gcttcacgct cggcgcgcat
    22621 acggttgtag atcgcttcag acacttcggt cggcaggttg atctgcttga tacgcacatc
    22681 gacaacttca atacccagcg ccgccatact gttcgggttg atgaccggaa ctttgccctt
    22741 cgtctcagcc gttacgcgct ctgccgcttc ggcaatggcg ttatccgctg ccggggtagt
    22801 aacttcatct tctgtacccg cagaaccgga gttcagcgcg tcacgtactt cgagggtcag
    22861 acgaccacgg gaatcggtga cgatgtcttt cacgtccagg cgaccaattt cagaacgcag
    22921 acggtcagag aacttacgtt tcaacagcac ttccgcttgc gaaatgtcgc caccacccgt
    22981 tgccaggtag taacggctga aatcgctgat gcgccatttg atgtaagaat cgacgatcag
    23041 gtctttcttc tctttggtca caaagcggtc ggcctggttg tccatggtct gaatacgtgc
    23101 gtcgagcatt ttcaccgttt caatgaacgg tatcttgaaa tgcagacccg gctcataaac
    23161 cagaggtttg ttgtcatcgt cacgcagtac cttaccaaaa cgcagcgtaa taccgcgctc
    23221 accttctttg acgacaaaga cagacatgta aagcactacc agcacgatga tgataatcgc
    23281 gataactgac ttacgcatcg ttattccccc tgacgctggt agtcgttacg ctgcgcgttg
    23341 gcgcggcgtt ggtccataat atcgccctga ctggtggacg acgtgttgct tgctccactg
    23401 gttgtggagg aagaggctgg cggcaaacgc agcagattgc tggcaccgtt atcgctcttc
    23461 gccgcagggg cgttaccacc tttcagcatc tggtctaacg gcagaaccat caggttgcca
    23521 cctttatcgt taaccagcac tttgcgggtg ttacccaaca ctttttccat cgtctcgata
    23581 tacagacgct cgcgagtaat ttccggcgcg gctttatatt ccggcagaag tttagcaaag
    23641 cgcgccactt caccctgagc ttccaggatg gtctgggcct tgtacgcacg cgcctcttcg
    23701 aggatacgtt gcgcctgacc gttcgcacgc ggctgaactt cgttggtata cgcttctgct
    23761 tcacgaatgt attgctgttc gttttcacgc gcggcaatcg catcgtcaaa cgccgctttt
    23821 acttcttccg gcggacgagc agcctggaag ttgacgtcca gcagcgtgat acccatgtca
    23881 tacggacgga tagtctcttc cagttcgcgc tgagtatcgc tacgaatcac ggtacgacct
    23941 tccgtcagaa tgcggtccat ggtgtattta ccgataactc cacgcagggc gctgtcggta
    24001 gcctgacgca ggctgtcatc cgggctggtc acgctataca gatatttttc cggattggtg
    24061 acgcggtact gcacgttcat ctcaacgcgc actacgttct cgtccgacgt cagcatcaca
    24121 ccagaagcgg ccagttcacg cacggcttcc acgttcaccg gtttgacttc gtcgataaac
    24181 gtcggtttcc agttcagacc cggctcaacc agatggctga atttaccaaa gcgtgttacc
    24241 acgccgcgtt cggcttcttt aatggtatag aaaccactgg ccgcccagat aatgacaatc
    24301 gctgccgctg cgatggtaac gacacgaccg ccaagctgcg ggcgcgggcc ttgcgatgaa
    24361 ctgccaccgc cagatccggt gcctttaccg ccgcccagac caccgagctt tttgctcagt
    24421 ttgcggaaga tatcatctaa atcaggtggc ccttgatcgc gaccgccttt gtttccattt
    24481 ccctcagagt tgccgccagg tttgctgctt ccccacgggt cgcggtcttg tccgttatta
    24541 ccgggctgat tccacgccat gtttgtgctc catatttgtt atgcggtgat ccctgttgtc
    24601 tttcgacgcc agaggatatg actccacgct tcagacgcta cgccgttaga tcaggtaatc
    24661 gatcaacgcc ggttcttgtt tacagaggcg acgccagtca acgatcggca tacgaacttg
    24721 cagacttacg ctgccgtcct cctccatcca ctctttttct attgcctgaa gctgataaaa
    24781 acgacttctc agacgccctt cctgcggtgg cagacgcaat gtatgctgcg ccacctcgcc
    24841 ggaaagccgc tccgtcaaag cctgaaaaag ctgtggtatc cccgctccgg tctgtgcgga
    24901 aagccagaca cggatcggtt tgttctcttc gtcccgatca atacgcggtt cgaaatcttc
    24961 cagcatatcg atcttgttca tcaccagcag ggttgggatc tcgtgagcgt cgatctcttc
    25021 aagaaccgta ttcaccgctt cgatgttttc ttgtacacgc acatccgccg catcaatgac
    25081 gtgcagcagt aatgtggctt gccgcgtctc ttgtaacgtg gctttaaatg ccgccaccag
    25141 atcgtgcggc aggtggcgaa taaaccctac ggtatctgca agtacggttt caccgacatc
    25201 tgcaacgtca atacgccgca acgtcgggtc gagggtggca aacaactggt ctgccgcgta
    25261 gacccgcgct tcggtgatgc gattgaaaag ggtagattta ccggcgttgg tatatcccac
    25321 cagcgaaaca gtaggaacgt cggctttgat acgcgattgc cgcccctgct cacgctgctt
    25381 ttcaactctt tccaggcgtg actgtatctg cacgatgcga ttacgcaaca aacgacggtc
    25441 ggtttcgagc tgggtttcac ccggaccacg caaacctatc ccgcctttct gtctttcaag
    25501 gtgggtccag ccacgcacca ggcgcgtagc cagatggcgc agctgcgcca gctcaacctg
    25561 caacttaccc tcatgggtac gcgcacgttg ggcgaaaata tctaaaataa ggccggtgcg
    25621 gtcgataaca cgacactcgc acaaacgctc caggttacgc tcttgcgccg ggctcagggc
    25681 atggtcaaaa agaacgaccg aagcacccgt cgctttgaca gcttccgcaa tttcaactgc
    25741 tttaccttca cctacaaaat actttgggtg cggcgcttta cggctaccgg taatcacctg
    25801 caatgcttcg acaccggcgg aagagaccag agattcaaac tcctggaggt cttccatatc
    25861 tttgtcttgc gtaaaataga tgtgtaccag taccgcctgc tcaccagcat cataacggtc
    25921 aaacaagcgt ataaccctct aaatagatca gcggggaacg caggatcgct ggctccccgt
    25981 gtaaaaaaac agcccgaaac cttattcggt ttcttcgctg tcctgttgcg cggaagtatt
    26041 ctgcgcgctg ctaccatgat ggtagttact gctggtaccg ccaccggcgt tgttactgtg
    26101 atgagaaacc gggcgagacg ggacaacagt agaaatcgcg tgcttgtaaa ccatctggct
    26161 gaccgtgttt ttcaacagga tcacgaactg atcaaaagac tcgatttgcc cttgcagctt
    26221 aataccattc accaaataaa tagaaactgg aacacgttcc cgacgcagtg cgttcaggaa
    26281 cggatcttgt aaagattgcc ccttagccat tctctctttt ccttatatgc ttatttgtac
    26341 tttgaacctt tcgattctga aaaaattgcg cacgatacgt ctcaattgta cacattcagc
    26401 ctgcgatagc accaacaacc tgtaatactt cgtcacgcgc ctgttctggt ttttcactgt
    26461 caagccagtg aaccccttcc caaccacgca gccaggttat ctgccgcttc gccaactgtc
    26521 tcgtggcgca aacacctcga taaaccattt catcgtatga gatttcgcct tcaaggtaag
    26581 accacatctg gcgataaccc acgcaacgaa tggaaggcaa gtccgtatgc aaatctcctc
    26641 gggcaaaaag cgcccggact tctgcttcaa aacctgaagc caacatctga tgaaaacgct
    26701 gctcaatgcg ttgatggagc agttcacggc tcgccggggc gatggcgaac tgatgcacct
    26761 gatacggtag agcgtctcct gacgtttgcg tcagttccgt taaagtttta cccgaaatga
    26821 aaaaaacttc cagtgcccgg gaaagccttt gtggatcatt tggatgaatc cttgctgccg
    26881 caaccggatc tacctcctga agttgacgat gcaatgactc ccaaccttgc tctgccgcct
    26941 gttgctcaat tctggcccgc acttccgggt ctgccgacgg tagcggcgac aacccttcca
    27001 gcaatgcctt gaaatacaac atcgtaccgc ccactaacag tgggatccgc cccgccgcgg
    27061 tgatatcggc catttccgcc agcgcatcgc ggcgaaaatc agcagccgag taagcctgcg
    27121 acggatcgcg aatatccagc aatcggtgcg gcgcggcgag taactcttca gcgttcggct
    27181 tcgccgtccc gatatccatc cctttgtaaa taagggcaga atcaacgctt atcaactcta
    27241 ctggtaaaat tttacgcagc tcaatggcta acgccgtttt accggaggcc gtcggcccca
    27301 tcaaaaaaat cgccttaggc aggctcgcct tactgatatc actcatcttt cagggctttt
    27361 atcgccggat gtaaatcaac agattgtaac agaccacccg gcggcgtttt cacaagttgc
    27421 ggacataacc gttccacgtc cgccagcagg gttatggcct gtgccattga ccactgcgca
    27481 tgttcgctca tcagatttcg tgcaatccac tgcgcaatat tgccaggttc gaatacggac
    27541 tgcttcgcca ggtagcctat cagttcagga atcaagattt gtaaattttg ttggcgtaag
    27601 ggtaaaggca cggccctgat ggtcacatgc tgtgcatctg actggaaatc aatacccaat
    27661 tccgccaggg cagactgcgc tttttctaat gccgattttt cttcggcaga aacttttagc
    27721 cgcaacggaa tcagcagcgg ctgggcgcaa acgggcgctt cacccggcgt caattgtgcc
    27781 tgacgtagcc aacgttctgc cactggcaag gataaaagtg aaatgttgcc atcgcgctcc
    27841 agcaacgcac agtcggaatg gacgatagtc agtacccgac caaaactctg actgttcgcc
    27901 gcaagtgcag gttcctgcgg ttccggcgct tttggttttt gcatcggcgc gggcgtttgc
    27961 aaaagctggc gatacacttc accttgctgt ttctggtagc ctggctgcgc attcggccag
    28021 ggggcagccg gacgactgcc tgatgctggc gcaggagtgt agcgcggagc taccggctca
    28081 cgagctgccg gttctgcaaa gtgattgcgc cccgccgcca cgcggttttc cggaatggaa
    28141 cgcggtgcag gttggggttc atcgtccagc ggtagcggcg tttccagttg ctgttgtagc
    28201 acgctcagca cgccctgata gataaaatca tgcaccagac gcgactgatg gaaacgcact
    28261 tcgtgtttgg cggggtgcac gttgacgtcc acctgatgcg gatcgatctc cagatacagc
    28321 acaaatgccg gttgctgatc ggcccccagt ttgtcttcgc aggcctggcg gatcgcgtga
    28381 ttgatcaggc gatcgcgcat catgcgaccg ttcacgtagc aatactgaat ttctgccagt
    28441 gcgggcgtgg tgtgatttgg atcggccacc cagccgcgta gcgtgagatc gccgtgttgc
    28501 cattcaatcg ccagcgcttg ttcaagaaaa gcggtgccgc aaatcgcgcc taagcgccgt
    28561 tctttttgcc cgccttccgg cactgcgcgg tactgacgca caattttacc gttatgcgac
    28621 aggttgatcg tgacgtcgaa acgcgccagc gcaatgcggc ggatgatctc atcaatgtgg
    28681 ttaaattcgg ttttctcggt gcgcaggaat ttgcgccgcg ccggggtgtt gtagaacaga
    28741 tccagcacct ccagcgtcgt ccccacagga tgcgccgccg gttttaccgt cacgttcata
    28801 tcgcgccctt cggcataggc ctgccaggct tcctgctgtt ctgcggtgcg tgaagtgagc
    28861 gtcaggcggg aaaccgaact gatactcgcc agcgcctcac cgcgaaagcc caggctgata
    28921 atggcttcga gatcgtccag agaggcgatt ttactggtgg catgacgcgc cagcgccagc
    28981 gccagctcgt cttttttgat accgcagccg ttatcacgaa tgcggataag tttcgcccca
    29041 ccgcgttcga tatcaatatc gatacgtgtc gcacctgcat cgaggctgtt ttccaccagt
    29101 tctttgacta ccgacgcagg tcgctcgacc acctcacctg cggcaatctg gttcgccagt
    29161 tgtggcggta agacctgaat tggcatcaat cgtccttagt ttggcagcgt gcgatctggc
    29221 gtcgtcaccg tactggccgt ttgtgccgtt gccccctgcg gcgcagattg catcggatgc
    29281 gcaaggaaat aattgcgcag gcctttgtaa atggcttctg ccagctgttg ttggtaatcg
    29341 tcgctcgcca gcaaacgttc ttcgctgttg ttgctgataa aaccggtttc gaccagtact
    29401 gatgggatat ccggcgaacg cagaacgccg agactcgcgt gttctggtcg gcgtttatgt
    29461 atttcgccaa tgcgttgcaa ctgactgatc atactggtcg ctacatcata ccctacccgt
    29521 tgggaatgac cgaactgtaa atccagcacc gcctggctta aataggggtc agactgactg
    29581 ttcgccagca catcacccgc cccacccagc agctccgact gtttctcgtg ctgctccagc
    29641 cagctggcca tttcgctgtt ggcgcgacgg ttagagagca cccatacgga agcgccagtc
    29701 gcactgcggt tcggtgcggc atcagcgtga atcgacacga ggaaattggc gttttgctta
    29761 cgtgccacat cactgcgccc catcaccgag ataaagtaat ccccgtcacg ggttaaaacg
    29821 cccttaaaca tcggatcgtc attgagcaaa gtacgcaatt tacgcgcgat ggcgatggtg
    29881 acatttttct cccgcgtacc accggggccg atagcgccag ggtcctgacc gccgtgtccg
    29941 gcatcaatag cgatgataat tttatcgcca gtgttagccg tcgcgcgcgc tgccggacgc
    30001 gttaccgtat tactgctgat aacacccgta gtgcggttac tttccgtttt aaacggattg
    30061 cgcgccggtt cgctgacgcg cggtgcgaca accgcaggcg tttcaacgcg tttcgcaacc
    30121 acgggcggcg gaggaggagg tggcggcgca tcggcgttaa tcgtaaagac gacagtgtaa
    30181 ttactgccat tctgccgctt caccgcttcg gttttaccgt tttcggtaag atcgaccacc
    30241 agccgtagcg tttgtgcatc tttaggcgtt ccagagcgaa tcgccttcac cagattattg
    30301 ccgctgaaca acaacggcag tccctgaatc acgcccgttt gtttgatatc gagcgccacg
    30361 gtgcgtttgc tttgatggct aaacgcataa tcaggatcgc caataaaact caacgttatc
    30421 cgcgcctgtt gattaccgtt agaaacctga atatcagaga gcgtcgcggc acccaccggc
    30481 gtgcacagca gcagcagcgt cgctaccaac caatttctga tgcgatacat catcccgcca
    30541 cctttcaaag gttaaccggc taaacgcgcc agcaacaatt cacccgcaga ggaaaccgca
    30601 ctcacgcgcg cctcacggcc ttgtgcctga taatcaatgt gtatttcgac atccgggtca
    30661 ggaagaacac cagtaccttg ttgtggccac tccaccaggc agatggcatc gttggcaaaa
    30721 taatcgcgaa tccccataaa ctccagctcc tcgggatcgg caaggcggta caaatcaaag
    30781 tgataaacca ttaaattgtc gagcgtatag ggttcgacca gcgtataagt ggggcttttg
    30841 acattaccct gatgacccag agcttgtaaa aagccccggc taaaggtggt tttacctgcg
    30901 cctaaatcgc catacagata gattacggtt gcgccatcgc aggctttcgc tacccgctcg
    30961 cccaggtcta atgttgcctg ctcatcaggg agcggaatta ctcgattcat catggttttt
    31021 atcagtcact tccgggttaa caatacgctg tagcgtggaa aagagatcgg ttgccagcat
    31081 cccgcgcgtt ccaaaacgcg ccgccagtac gtcagctgca gcaccgtgcg cgacacagcc
    31141 cgcacaggcg gcatcatacg gcgacagttt ttgcccaagc aatgcgccaa taataccaga
    31201 gagcacatcg cccatgccgc cgctcgccat gcctgcattt ccggcatcaa taatgcctaa
    31261 agcgtcagga tgggcggcga ccacggttcc ggcacctttc agcaccgcta cgccgccata
    31321 acgttgtacc agacgtttgg cgcaatgtaa gcggtcactt tcaatttcag cgacggaaca
    31381 gcctaacaac cgtgcggcct cgccaggatg cggcgtgatc acgcgattgt gacgcttatc
    31441 gggattgatt gccagcaggt tcaatgcatc ggcatcccac aacatcggtt tgcgaaaatt
    31501 ctcaactttt tgcagtgctt ttttccccca ctcttgctgg cccagaccgg gaccaatcac
    31561 caccacatcg gcccattcca ggctttcggt aagagagtcc atcgtcagtt catgcaccat
    31621 caattccggt cgtgcagtca gcagcggcgc aatgttttca ctgcgggtca gtactcggac
    31681 taaaccagca ccagcacgta gcgccgcttc ccccgtcata cgaatagccc ccgccgtgcc
    31741 gtgatcgcca ccgataatta ccagccgccc gtgatcgcct ttatgcgaag tcgggcgacg
    31801 cggttttagc cagtgagaaa gttgttctgc cgaaaaccgc tgaattttcg tctcctgacc
    31861 tgccagccaa ctatccagcc ccagtgagtc aaaatgcagt tgtccggtaa catcccgcgc
    31921 ttttccagtg agcaagcctg gtttcagcgc aataaaagtg atggtgtgat cggcgttgat
    31981 cactgcgcct ggcgtagcgc cggtttcagc cagcaggccg gaagggatat caaccgccgc
    32041 aatcggcgca ggatgggaat tagcgtggtc gattaactgg ctaatggatt cgcggggcgc
    32101 ttgctgcaag ccggtaccga gcagcgcatc aacaatcaga tctaccgatt cgggccagac
    32161 aatattcgaa gcatggatct cgcctcccgc gtttaaccat gcttcgcgtg ccagcgcggc
    32221 ctcttccggc aacggtttgt cgctctcctg ggccagcagc gtgacctcaa tgccgaccgc
    32281 tgtggccagc cgcgcgacca cgtagccatc gccgccgtta ttaccatgac cgcacagcac
    32341 cagccaatgg cgggcgtcag gatacgccga acgacacacc tggaatgcgg cttcgccagc
    32401 gcgaagcatc agctcataga gtgtaagccc cagcgcatct gccgcctcac gttctccgcg
    32461 gcggatatcg tcggcgtgcc agacggtgtg tggtatactt acggggtttt tcttcattgt
    32521 atggtccgtc atgtcagagc ccctcgatct caatcagtta gcgcaaaaaa ttaaacagtg
    32581 ggggctggaa ctgggctttc agcaggtagg tattaccgat accgatctca gcgagtccga
    32641 gcccaaactg caagcatggc tggacaaaca ataccacggc gaaatggact ggatggcacg
    32701 tcacggtatg ctgcgcgctc gcccccatga gttattgccc ggtacgctgc gcgtgatcag
    32761 cgtgcggatg aattaccttc ctgctaacgc cgcatttgcc agcacgctga aaaaccccaa
    32821 actcggctat gttagccgtt atgcgctggg ccgtgactat cacaaacttc tgcgcaaccg
    32881 actcaaaaag ctgggcgaga tgattcagca acattgtgtt tcgctgaatt ttagaccgtt
    32941 tgtcgattct gcgcctattc tcgagcgccc gttagctgaa aaagctgggc tcggctggac
    33001 aggtaagcac tcacttatcc tcaatcgcga ggccggttcg ttcttctttt taggcgaatt
    33061 gctggtcgat attccgctgc ccgtggatca accagtcgag gaaggatgcg gcaaatgcgt
    33121 ggcctgtatg acgatttgcc cgaccggtgc catcgtcgag ccatataccg tcgatgctcg
    33181 ccgctgtatc tcttatctca ccatcgaact ggaaggggcg atcccggaag agttgcgacc
    33241 gttaatagga aaccgtattt acggttgcga tgactgccag cttatctgcc cgtggaatcg
    33301 ctattcacaa ctcaccacag aagaggattt cagcccgcgt aagccgctac acgcaccgga
    33361 actcattgag ttattcgcct ggagcgaaga gaagttttta aaagtcacgg aaggatcggc
    33421 gattcgtcgt attggtcacc tgcgttggct gcgtaatatc gccgtagcat taggcaatgc
    33481 cccttgggat gaaacgattt tgacagcgct ggaaagtcgt aaaggtgagc acccacttct
    33541 cgatgagcac atagcgtggg cgattgcgca gcaaatagag agacgaaatg cgtgcatagt
    33601 cgaagtgcaa ttgccgaaaa aacagcgtct ggttcgggtg attgaaaaag ggttaccgcg
    33661 tgacgcctga ttcatccaca gcttgtgaat aaaaataaaa acgcattgca attcaagacg
    33721 aataaaatcg tcaagtgatc gactcgacaa aacggaatct taatttttaa cttatctatc
    33781 aataggttaa aaaaatacta ttcaccgtgc gaagtttctt tgaaattaag attacagcct
    33841 gaggctgtgg ataactctgt ttacaagagt ttgtcagaga caacgaaaac cagcaataac
    33901 gcatcgcatc gctgtggata ttttattgag agaagaattt g
//
LOCUS       DAZFQD010000039        33641 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.039, whole genome shotgun sequence.
ACCESSION   DAZFQD010000039 DAZFQD010000000
VERSION     DAZFQD010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33641
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.039"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(201..392)
                     /gene="yfgG"
                     /locus_tag="ACHKTW_001963"
     CDS             complement(201..392)
                     /gene="yfgG"
                     /locus_tag="ACHKTW_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfgG family protein"
                     /protein_id="HFO6462995.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
     gene            410..562
                     /locus_tag="ACHKTW_001964"
     CDS             410..562
                     /locus_tag="ACHKTW_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6462996.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLCKKASVDTYFNVMEVNLIIYND
                     DVIMMN"
     gene            744..2987
                     /gene="pdeF"
                     /locus_tag="ACHKTW_001965"
     CDS             744..2987
                     /gene="pdeF"
                     /locus_tag="ACHKTW_001965"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HFO6462997.1"
                     /translation="MKLNATYIKIRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGITLGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDTRLISQRIFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDAKVTKKEFAL
                     WLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMI
                     SIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRAVVRRIQRLAYVDP
                     VVHLPNVRALNRALRDAPWSALCYLRIPGMEMLVKNYGIMLRIQYKQKLSHWLSPLLE
                     PGEDVYQLSGNDLALRLNTESHQERITALDSHLKQFRFFWDGMPMQPQIGVSYCYVRS
                     PVNHIYLLLGELNTVAELSIVTNAPENMQRRGAMYLQRELKDKVAMMNRLQQALEHNH
                     FFLMAQPITGMRGDVYHEILLRMKGENDELIGPDSFLPVAHEFGLSSSIDMWVIEHTL
                     QFMAENRAKMPAHRFAINLSPTSVCQARFPVEVSQLLDKYQIEAWQLIFEVTESNALT
                     NVKQAQITLQHLQELGCQIAIDDFGTGYASYARLKNVNADLLKIDGSFIRNIVSNSLD
                     YQIVASICHLARMKKMRVVAEYVENEEIREAVLSLGIDYMQGYLIGKPQPLIDTLNEI
                     EPIRESA"
     gene            complement(3026..4567)
                     /gene="ppx"
                     /locus_tag="ACHKTW_001966"
     CDS             complement(3026..4567)
                     /gene="ppx"
                     /locus_tag="ACHKTW_001966"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006798 - polyphosphate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HFO6462998.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEVLRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(4572..6638)
                     /gene="ppk1"
                     /locus_tag="ACHKTW_001967"
     CDS             complement(4572..6638)
                     /gene="ppk1"
                     /locus_tag="ACHKTW_001967"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HFO6462999.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNSHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRGDTIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDFIK
                     SLEQPE"
     gene            complement(6809..7447)
                     /gene="purN"
                     /locus_tag="ACHKTW_001968"
     CDS             complement(6809..7447)
                     /gene="purN"
                     /locus_tag="ACHKTW_001968"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFO6463000.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            complement(7447..8484)
                     /gene="purM"
                     /locus_tag="ACHKTW_001969"
     CDS             complement(7447..8484)
                     /gene="purM"
                     /locus_tag="ACHKTW_001969"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFO6463001.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNSNGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            8809..9435
                     /gene="upp"
                     /locus_tag="ACHKTW_001970"
     CDS             8809..9435
                     /gene="upp"
                     /locus_tag="ACHKTW_001970"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFO6463002.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            9521..10810
                     /gene="uraA"
                     /locus_tag="ACHKTW_001971"
     CDS             9521..10810
                     /gene="uraA"
                     /locus_tag="ACHKTW_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HFO6463003.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPDSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            10905..11606
                     /gene="hda"
                     /locus_tag="ACHKTW_001972"
     CDS             10905..11606
                     /gene="hda"
                     /locus_tag="ACHKTW_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HFO6463004.1"
                     /translation="MNTPAQLSLPLYLPDDETFASFWPGDNSSLLAALQNVLRQEHSG
                     YIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVPEVLDGMEHLSLVCID
                     NIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNLGLPDLASRLDWGQIY
                     KLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRTLFMTLDQLDRASITA
                     QRKLTIPFVKEILKL"
     gene            complement(11744..12103)
                     /gene="arsC"
                     /locus_tag="ACHKTW_001973"
     CDS             complement(11744..12103)
                     /gene="arsC"
                     /locus_tag="ACHKTW_001973"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311384.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HFO6463005.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIVVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(12124..13587)
                     /gene="bepA"
                     /locus_tag="ACHKTW_001974"
     CDS             complement(12124..13587)
                     /gene="bepA"
                     /locus_tag="ACHKTW_001974"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HFO6463006.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQERFKPYTKM"
     gene            13800..14861
                     /gene="yfgO"
                     /locus_tag="ACHKTW_001975"
     CDS             13800..14861
                     /gene="yfgO"
                     /locus_tag="ACHKTW_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFO6463007.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPALMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            complement(14899..15747)
                     /gene="focB"
                     /locus_tag="ACHKTW_001976"
     CDS             complement(14899..15747)
                     /gene="focB"
                     /locus_tag="ACHKTW_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocB"
                     /protein_id="HFO6463008.1"
                     /translation="MRNKLSFDLQLSARKAAIAERIAAHKIARSKVSVFLMAMSAGVF
                     MAIGFTFYLSVIADAPSSQALTHLVGGLCFTLGFILLAVCGTNLFTSSVMTVMAKSRG
                     VISWRTWLINALLVACGNLAGIACFSLLIWFSGLVMSENAMWGVAVLHCAEGKMHHTF
                     TESVSLGIMCNLMVCLALWMSYCGRSLCDKIVAMILPITLFVASGFEHCIANLFVIPF
                     AIAIRHFAPTSFWQLAHSSADNFPALTVSHFITANLLPVMLGNIIGGAVLVSICYRAI
                     YLRQEP"
     gene            complement(15769..17781)
                     /gene="hyfR"
                     /locus_tag="ACHKTW_001977"
     CDS             complement(15769..17781)
                     /gene="hyfR"
                     /locus_tag="ACHKTW_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
                     /protein_id="HFO6463009.1"
                     /translation="MAMSDEAMFAPPQGITIEAVNGMLAERLAQKHGKASLLRAFIPL
                     PPPFSPVQLIELHVLKSNFYYRYHDDGSDVTATTEYQGEMVDYSRHAVLLGSSGMAEL
                     RFIRTHGCRFTPQDCTLFNWLARIITPVLQSWLNDEEQQVTLRLLEKDRDHHRVLVDI
                     TNAVLSHLDLDDLIADVAREIHHFFGLASVSMVLGDHRKNEKFSLWCSDLSASHCACL
                     PRNMPGDSVLLTQTLQTRQPTLTHRADDLFLWQRDPLLLLLASNGCESALLIPLTFGN
                     HTPGALLLAHTSSTLFSEENCQLLQHIADRIAIAVGNADAWRSMTDLQESLQQENHQL
                     SEQLLSNLGVGDIIYQSQAMEDLLQQVDIVAKSDSTVLICGETGTGKEVIARAIHQLS
                     PRRDKPLVKINCAAIPASLLESELFGHDKGAFTGAINTHRGRFEIADGGTLFLDEIGD
                     LPLELQPKLLRVLQEREIERLGGSRTIPVNVRVIAATNRDLWQMVEDRQFRSDLFYRL
                     NVFPLELPPLRDRPEDIPLLAKHFTQKMARHMNRSIDAIPTEALRQLMSWDWPGNVRE
                     LENVIERAVLLTRGNSLNLHLNVRQSRLLPTLNEDSALRSSMAQLLHPTTPENDEEER
                     QRIVQVLRETNGIVAGPRGAATRLGMKRTTLLSRMQRLGISVREVL"
     gene            complement(17811..18224)
                     /gene="hyfJ"
                     /locus_tag="ACHKTW_001978"
     CDS             complement(17811..18224)
                     /gene="hyfJ"
                     /locus_tag="ACHKTW_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HFO6463010.1"
                     /translation="MTEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVAFPCPLTEYEDWLAQVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFAPLA
                     DDATSVWQARSIQFIHLLDEIVQEPAIYLMARKIA"
     gene            complement(18217..18975)
                     /gene="hyfI"
                     /locus_tag="ACHKTW_001979"
     CDS             complement(18217..18975)
                     /gene="hyfI"
                     /locus_tag="ACHKTW_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
                     /protein_id="HFO6463011.1"
                     /translation="MSPVLTQYVSQPITLDEQTQKMKQHLLQDIRRSAYVYRVDCGGC
                     NACEIEIFAAITPVFDAERFGIKVVSSPRHADILLFTGAVTRAMRMPALRAYESAPDH
                     KICVSYGACGVGGGIFHDLYSVWGGSDTIVPIDVWIPGCPPTPAATIHGFAVALGLLQ
                     QKIHAVDYRDPTGVTMQPLWPQIPPSQRIAIEREARRLAGYRQGREICDRLLRHLSDD
                     PTGNRVNTWLRDADDPRLNSIVQQLFRVLRGLHD"
     gene            complement(18972..19511)
                     /gene="hyfH"
                     /locus_tag="ACHKTW_001980"
     CDS             complement(18972..19511)
                     /gene="hyfH"
                     /locus_tag="ACHKTW_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HFO6463012.1"
                     /translation="MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACPANALTIQTDDQQNSRTWQLYLRRCIYCGRCEEVCPTRAIQLTNNFELTVTNKA
                     DLYTRATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLRAQASVCPECKQ
                     RATLINDDTDVPLVAKEQL"
     gene            complement(19521..21236)
                     /gene="hyfG"
                     /locus_tag="ACHKTW_001981"
     CDS             complement(19521..21236)
                     /gene="hyfG"
                     /locus_tag="ACHKTW_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
                     /protein_id="HFO6463013.1"
                     /translation="MNVNSSSNRGEAILAALKTQFPGAVLDEERQTPEQVTITVKINL
                     LPDVVHYLYYQHDGWLPVLFGNDERTLNGHYAVYYALSMEGAEKCWIVVKALVDADSR
                     EFPSVTPRVPAAVWGEREIRDMYGLIPVGLPDQRRLVLPDDWPEDMHPLRKDAMDYRL
                     RPEPTTDSETYAFINEGNSDARVIPVGPLHITSDEPGHFRLFVDGEQIVDADYRLFYV
                     HRGMEKLAETRMGYNEVTFLSDRVCGICGFAHSVAYTNSVENALGIEVPQRAHTIRSI
                     LLEVERLHSHLLNLGLSCHFVGFDTGFMQFFRVWEKSMTMAELLTGSRKTYGLNLIGG
                     VRRDILKEQRLQTLKLVHEMRADVSELVEMLLAMPNMEQRTQGIGILDRQIARDYSPV
                     GPLIRGSGFARDLRFDHPYADYGNIPKTLFTFTGGDVFSRVMVRVKETFDSLAMLEFA
                     LDNMPDTPLLTEGFSYKPHAFALGFVEAPRGEDVHWSMLGDNQKLFRWRCRAATYANW
                     PVLRYMLRGNTVSDAPLIIGSLDPCYSCTDRVTLVDVRKRQSKTVPYKEIERYGIDRN
                     RSPLK"
     gene            complement(21226..22806)
                     /gene="hyfF"
                     /locus_tag="ACHKTW_001982"
     CDS             complement(21226..22806)
                     /gene="hyfF"
                     /locus_tag="ACHKTW_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HFO6463014.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGLSATCTVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     AHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLEAAWKYIIICTVGVAFGLFGTVLVYANVASVMPQAEMAIFWSEVLKQSSLLDPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHTLNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALAGMPPFNIFLSEFMTVTAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKPPQ
                     AVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQRSTW
                     HDFLPSGTASVSEKHSER"
     gene            complement(22811..23461)
                     /gene="hyfE"
                     /locus_tag="ACHKTW_001983"
     CDS             complement(22811..23461)
                     /gene="hyfE"
                     /locus_tag="ACHKTW_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HFO6463015.1"
                     /translation="MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFAAEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            complement(23473..24910)
                     /gene="hyfD"
                     /locus_tag="ACHKTW_001984"
                     /pseudo
     CDS             complement(23473..24910)
                     /gene="hyfD"
                     /locus_tag="ACHKTW_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416979.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfD"
     gene            complement(24927..25874)
                     /gene="hyfC"
                     /locus_tag="ACHKTW_001985"
     CDS             complement(24927..25874)
                     /gene="hyfC"
                     /locus_tag="ACHKTW_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311372.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HFO6463016.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLMLTPLFTGISRQIRARMHSRRG
                     PGIWQDYRDIHKLFKRQEVAPISSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLATGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGLGLKQVVMAALFVALFLPFGRAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            complement(25885..27902)
                     /gene="hyfB"
                     /locus_tag="ACHKTW_001986"
                     /pseudo
     CDS             complement(25885..27902)
                     /gene="hyfB"
                     /locus_tag="ACHKTW_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311371.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
     gene            complement(27902..28519)
                     /gene="hyfA"
                     /locus_tag="ACHKTW_001987"
     CDS             complement(27902..28519)
                     /gene="hyfA"
                     /locus_tag="ACHKTW_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HFO6463017.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     LTAPVVCHHCEEAPCLQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAHAQYVFQAEGSLKDGEENVLPQHALLRREPGVQTVAVKCDLCDFLPEGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGGEDPLSLTQEQH"
     gene            complement(28772..29242)
                     /gene="bcp"
                     /locus_tag="ACHKTW_001988"
     CDS             complement(28772..29242)
                     /gene="bcp"
                     /locus_tag="ACHKTW_001988"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HFO6463018.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            complement(29242..29814)
                     /gene="gcvR"
                     /locus_tag="ACHKTW_001989"
     CDS             complement(29242..29814)
                     /gene="gcvR"
                     /locus_tag="ACHKTW_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HFO6463019.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            29960..30838
                     /gene="dapA"
                     /locus_tag="ACHKTW_001990"
     CDS             29960..30838
                     /gene="dapA"
                     /locus_tag="ACHKTW_001990"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFO6463020.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLELADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            30855..31889
                     /gene="bamC"
                     /locus_tag="ACHKTW_001991"
     CDS             30855..31889
                     /gene="bamC"
                     /locus_tag="ACHKTW_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HFO6463021.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VSVFQAAFSK"
     gene            32102..32815
                     /gene="purC"
                     /locus_tag="ACHKTW_001992"
     CDS             32102..32815
                     /gene="purC"
                     /locus_tag="ACHKTW_001992"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFO6463022.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            32969..33196
                     /gene="vapB"
                     /locus_tag="ACHKTW_001993"
     CDS             32969..33196
                     /gene="vapB"
                     /locus_tag="ACHKTW_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system antitoxin VapB"
                     /protein_id="HFO6463023.1"
                     /translation="METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAG
                     ESWDSWFDGENVSADFMDIRDQPAMQERESF"
     gene            33196..33594
                     /locus_tag="ACHKTW_001994"
     CDS             33196..33594
                     /locus_tag="ACHKTW_001994"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HFO6463024.1"
                     /translation="MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELI
                     YGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHTGQIRAELARQGRPVGPFDQMIA
                     GHARSRGLIVVTNNTREFERVAGIRLEDWS"
BASE COUNT         8321 a         8624 c         8765 g         7931 t
ORIGIN      
        1 ggaataattt ataaaaatat atcattacaa catatatatt aattttatat ttatttcgca
       61 gccaaacagg ataaacatca gtcaggacaa agacgtaaaa tttaagatga aagaatatct
      121 gccgctttga taattccaaa gagcaaaagc atccgacagc gaagtgccgt taggttctgt
      181 gaaagtggag aaggtaaccg ctaacgttgt accggtgatt cgacggagag tgtggattgc
      241 tgagcttctt ttttgctctg atggtgctgc caggcaccga cggacgagta gataaaacgg
      301 ccaaagaaga agataaagct gatgagcagt acgatacggg tcatgcgact gttaaatcgg
      361 tgtcgttttc gcatactggt tgcctgactc acaaaaggtt ccttgaagta tgtcccacgc
      421 tgtggacggt acttacatta aggcacaaca ggacaaaatg gtcaattctt cgttatgtaa
      481 aaaagcgtca gtggatacat attttaatgt tatggaagtt aatttaatta tttacaatga
      541 tgatgtaata atgatgaatt aacatgataa acgtaataat tcattaatca gggttatttt
      601 gtttgatata tatcaattgg aactttcata tgacacttag aatcaatgct ctctatagtg
      661 atgaataatc atcattcgaa gtcaggtggg atgcctgtct gaatacacct ccttcaggat
      721 gtgggggatt ctgctgagca tctatgaaac tgaatgcaac ttatataaaa atacgtgata
      781 aatggtgggg gcttccgctg ttcctgcctt ctttaatctt gcccattttc gcccacatta
      841 atactttcgc gcatatttct tccggtgagg tttttctctt ttatctgcct ttggcactga
      901 tgatcagcat gatgatgttt ttcagctggg cggcattgcc agggatcacc ttagggattt
      961 ttgtccgcaa atatgcagag ctgggttttt acgaaacgct ctcattaacg gctaatttta
     1021 ttatcattat cattctctgt tggggcggtt acagggtctt tactccccgg cgtaacaacg
     1081 tttcacatgg tgatacccgt ttaatttccc agcgtatatt ctggcagata gtgtttcctg
     1141 caacgctgtt tctgatactt ttccagtttg ctgcatttgt aggattgctg gcgagcagag
     1201 aaaatctggt cggtgtcatg ccttttaacc tcgggacttt aatcaattat caggccttac
     1261 tggtgggtaa tctgatcggt gtcccgctgt gctacttcat cattcgggta gtgcgaaatc
     1321 cattttattt acgtagctat tattcgcaat taaaacagca ggttgatgcc aaagtcacca
     1381 aaaaagagtt cgcgctctgg ctactggcat taggtgcttt attgttgctg ttatgcatgc
     1441 cgttaaatga aaaaagcaca atttttagca ccaattatac cttgtcatta ttgctgcccc
     1501 tgatgatgtg gggagcgatg cgctatggtt ataagctgat ttcgctgctc tgggcggtcg
     1561 tgttgatgat cagcatccac agctatcaaa attacattcc catttatcct ggctatacca
     1621 cgcagctgac cataacctcc tccagttatc tggtattctc ttttattgtc aattatatgg
     1681 ctgtactggc aactcgtcag cgagcggtag tcagacgcat tcagcggctt gcgtatgtgg
     1741 acccggtggt tcatctgcca aatgttcgcg ccctgaatcg cgcgttgcgt gatgccccct
     1801 ggtctgcgct ttgttattta cgcatccctg gcatggaaat gctggttaag aactatggca
     1861 tcatgctgcg gattcaatac aagcaaaaac tttctcactg gctgtcaccc ttgctggaac
     1921 cgggtgaaga tgtttatcag ctttcgggta acgatctcgc gctgcggctg aatacagaat
     1981 cgcaccagga gcgcattacc gcactggata gccatctcaa gcaatttcgt ttcttttggg
     2041 atggcatgcc gatgcaaccg cagattggcg tcagttactg ctatgtgcgc tcgccagtga
     2101 atcatatcta cctgctgctg ggagagctaa atacggtcgc cgaactttcc atcgtgacca
     2161 acgccccgga aaatatgcag cgtcgcgggg caatgtattt gcaacgcgaa ttgaaagata
     2221 aagtcgcgat gatgaatcga ctacagcagg cgctggaaca caaccatttt ttcctgatgg
     2281 cccagccgat taccggtatg cgtggtgatg tttaccatga aattcttctg cgcatgaaag
     2341 gtgagaatga tgaactgatc ggccccgata gcttcttgcc ggtcgcgcac gaatttggtt
     2401 tatcgtcgag tatcgacatg tgggtcattg agcatacgct gcaatttatg gctgaaaaca
     2461 gagcgaagat gcccgctcac cgttttgcta ttaatctgtc tccaacctcg gtatgtcagg
     2521 ctcgttttcc tgttgaagtc agtcagttgc tggataaata tcagattgaa gcgtggcaac
     2581 ttatttttga agtcaccgaa agtaatgctc tgaccaatgt taagcaggcg caaatcacct
     2641 tgcagcatct tcaggaatta ggctgccaga ttgcgattga tgatttcggc accggctacg
     2701 ccagctatgc gcggcttaaa aatgtgaatg ccgatctgct taaaattgac ggcagtttta
     2761 tccgcaatat tgtgtcaaat agtctggatt atcagatagt ggcatcgatt tgccacctgg
     2821 cgcgaatgaa gaaaatgcgg gtagtggcag agtacgttga aaacgaagag atccgcgagg
     2881 cggtgctctc tttggggatc gattatatgc agggttatct tattggtaag ccgcaaccgt
     2941 taattgatac gctgaatgaa atcgaaccca ttcgcgaaag tgcctgaata atgcgggccg
     3001 acatttctcg tcggcccgca aagcattaag cggcgatttc aggtgtactt tcttcttcaa
     3061 ttttcaaccg ccagccagcc acgccttccc agtattcttg ctccttttcc agatcaagca
     3121 gtaccagcgc attctgacta aaccagtcat gcgggaaacg cagtgtccag tgactgtcat
     3181 cagtaatcag tgtcaatgtt ggcggtgtgg tggttgcctg acgttgattg tttagtaata
     3241 cgccaaggcg caatagctgt atcagtggca ggaactgttt cttcttaaac aaggtaaagc
     3301 gcggcagatc gtcgagctta atcgctttac ggtgatagcg caccagtgtc gccatcatca
     3361 gctgctgttc ctgattaaaa cccggcaagt cactgttttg cagaatataa gcggagtggc
     3421 gatgcaaacc gctgtggttg atattcaacc cgacctcatg cagcatggcg gcccatcgca
     3481 gtagcgcctc cagttgcgga tgcgccagct tcggttgctg ttcccgccac tgttcgtaca
     3541 tttgcatagt ggtatccagc acccgtcggg cctgctcgct gtcgatgtga tactggttgg
     3601 cgaggctgct ggcggtgcga ctacgcacat cctgatgacg gaaacgtcct tccatttcat
     3661 acagtacgcc ttcgcgtaaa gccccgtcag aaaggcgcag ttcacggatg gctaaagcat
     3721 caaacacacc gcataaaatc gccagtccag gaacgaagac tgttttccgc tcttcggaaa
     3781 gacccggtaa actcagcgat gcgaaattac ggtgacgtaa aacttctttt accagttttt
     3841 ccagacgttc cggggtaatt atcccgtctt tctcgcccat ttccattaac acttcatggg
     3901 cggcttttat ggtaccggaa gcgcccattg ccacgttcca gccctgaata cggaattgcc
     3961 aggttaaagt ttccagtttt tgtgctgccg ccatacgagc gcgctgaaaa ttctctttat
     4021 tgatgacccc gccagggaag taaagctggg caaagctgac acaacccatc cggcggcttt
     4081 caacgagaat cggttcgaaa ttttcaccaa tcaccagttc cgtagatccg ccgccaatat
     4141 caataaccag tttgcgacct ttctccggtt gggtatgttc cacgcccata aaaatcagac
     4201 gggcttcttc attaccggaa ataatttcaa tcgggtaggg aatgaccttt tccgcgcgtt
     4261 tcagaaagtc agtggcgttc agcgcctgac gcagcgtatg ggtgccgact atacagacgc
     4321 tggcaggaga aaacccttgt agccgttcgg caaacagcga cagacagttc aaaccgcgcg
     4381 tcattgcctc ttcactcaac atattatctg gccccaggcc gtccgccaga tgcacccgct
     4441 gtttcaggcg gccaataatc tgcatggcac catctaccac acgggctatg accatgtgaa
     4501 aactgtttga accaagatcg accgcagcaa actcctgcgg acgaggggat ttatcgtgta
     4561 ttggcatagg gttattcagg ttgttcgagt gatttgatga agtcgtaaat cgccaactgc
     4621 gcccgtactt tgcggcgatt gccgcgggga acgtagcgat tactgagttc tttatcgata
     4681 taacgtgctt tgaccgtatc gctgaacaat atgtcgatga tgtccagcac ccgctgcttc
     4741 aggcgcggat cgagcagcgg cgtcgccact tcaatacgat aatcaatatt gcgcgtcatc
     4801 cagtcggcgg aagaaaggta gaccttttta tcgccgccat tttcaaaaat ataaacccgg
     4861 tcatgttcaa ggtaacggtc aacaatactg atggcacgaa tgttgtcgct aatgccttcc
     4921 agattgggga tcagcgaaca cattccgcga accagcagat taaccggtac gccggagctg
     4981 gaggccgcat acagacgatc aaccaggcct ttatcgacaa ggttatttag cttcagggtg
     5041 ataccgctgg gcagcccttg ctgcgcgttg gcgatttcgc ggtctaccat ttcatataac
     5101 agacggcgag agttttgcgg tgacaccatt aaataatcaa atgtcaccgg gcggtatggg
     5161 ttttcaataa agttaaatac ccgccgtact tcgttggtga tacgcgcatc ggcggtcagc
     5221 aatgaatagt cagtataaag acgcgcggtt ttttcgttaa agttcccggt cccgatgtgt
     5281 gcgtaacgca ccacttcacc gttttctttt cgtgaaatca ggaacagttt ggcgtgaatt
     5341 ttcagccccg gcgcagagaa gataacgtgc acgcctgctt cggtcaggcg cttcgcccag
     5401 tgaatgttag cttcttcgtc gaaacgcgcc tgtaactcaa ccaccacggt gactttctta
     5461 ccgttatgtg cggcgtggat catcgagtcg atgatgcgtg aatctttcgc cacgcggtaa
     5521 atgttaattt taatcgccag tacgctcggg tcgaacgaag cctgacgcag cagttccagc
     5581 acatgctcaa aggtgtgata aggataatag agcaacacat cgcgttcgcg aatggcatca
     5641 aaaccattgc ggaactgggc tttatcaaac caaatatggc gtaaacgcgg cagtggtttg
     5701 ttcaccagat tggctttgcc gacattgggg aaattaataa agtctttaaa attatgataa
     5761 cgaccgccgg ggacgatgga gtcgtagcgg gaaatagtca gtttttcgcg taacacttca
     5821 accagcgcat tgggcatatc gcgctgataa acaaaacgca ccggctcagc agttaaacgc
     5881 tgcttgagac tggaagacat caactccatc aggctggctt ccatctcatg cactaaatcg
     5941 tattcggcat cgcgggtcat cttcattgaa taggcattca gcgcgtcata atcaaagaag
     6001 cctttgaaaa tatcatcaag gcagtaacgc agaatgttat ccagaagaat catcggcttg
     6061 cgtcgacgcg gcgcttctgg cggtaaattc acaaagcgcg gcactttatc tgatgggatc
     6121 tccagcagcg cgtaacggat ggtatcgcca cggataattt ccaccgccag ataggtgtaa
     6181 tcatctttca ggaactgcac taagtcagtg tcaggattga ttaaaatcgg cgtaatgtgc
     6241 tgacgcagat actgcttaaa ataatgacgc agccagtttt gttgattgac ggagagctgg
     6301 cgttcattaa tcaggaagat ctggttacgc gccatctcca gcagcagctc gttgtagagg
     6361 ccgtcgaatt cctgatcggc tttcagcacc cgggactgaa ttttgcccag taaatggcgg
     6421 gaatgagagt tggagccttg ttcttcgcta ataatgatgc gtcgcttcag ttcagcgaag
     6481 cggactttat agaactcatc aaggttattg gaatagatcc ccaggaaacg catcctttca
     6541 atcagcgggt tagatttgtc cgccgcttcc tgaagcacgc gttcattgaa cgataaccaa
     6601 ctgagctctt tttcgatgta tagcttttcc tgacccatta ccacttttac tccgttttat
     6661 tcacgggaca ctgcctggat attatggcga gcaatattgg cagatgtcca actatgccag
     6721 aaaaggggga aaaaaactgc tggagcttgc gatttacagg tgaattgaaa aacgccagcg
     6781 gcagagctgg cgctttaact acggggcatt actcgtcggc agcgtagccc tgcggcggca
     6841 gacgttgacc atccagccac gcggcgtttt cgtgcatttt cagacgacca tcggcaaacc
     6901 agctaatcac cagtggataa atagcgtgtt cctgggtttg cacgcgggcg gtgatgtcat
     6961 cttccgtatc accagcaaat accgggactt tcgcctgtaa aataaccggg ccaccgtcca
     7021 gttcatcggt gacgaaatgc accgatgtac cgtgctcttc atcaccattt tccagcgcct
     7081 gacgatgggt gtgtaatccg ggatatttcg gcagcagaga agggtgaatg ttcagcaaac
     7141 gcccggcata gtgggagaca aacgccgggc tgagaatgcg cataaaacca gccagcacga
     7201 ccacatcggg tgcgtacatg tcgatttcat gaatcaactc ccggtcatag gcttcacgac
     7261 tgtcaaacgc gctggcgatg agcgtatgcg ttgcaatacc cgcctggcgg gcgcgttcaa
     7321 ggccgaacgc gtcggcctta ttgctgaaaa ctgcccgtac ggtgccttta attttgttag
     7381 ttttacaggc gtcaataatt gcctgtaaat tacttccgtt gccggaaata agcaccacaa
     7441 tattcattat tcgataacca cgcgttgttc ggaatcagag gctttgatga taccgatttt
     7501 ccacgcgttt tcaccgttgg agtttagcag ggcgagggct ttgtccactt ccggagcagg
     7561 cagggcgata atcatcccga cgccgcagtt gaaggtgcga tacatttcat ggcgctcaac
     7621 gttacctgcc gtttgcagcc agttgaacac ttccggccac tgccaggaag attcatcaat
     7681 cactgcctga gtattatctg gcaatacgcg tggaatgttt tcccagaagc cgccgccggt
     7741 caggtgcgca atggcatgca catcgacctt ttcaatcaac tccagcactg acttcacgta
     7801 aatgcgggtc ggtgccagca gatgatcggc taatggctta ccatcaagtt cggtggtttg
     7861 cggatcacaa ccgctgactt caagaatttt gcgcaccagc gaatagccgt tcgagtgcgg
     7921 accgctggaa ccgagtgcaa tcagcacatc gccgtcgctg actttagagc cgtcgatgat
     7981 ttctgatttt tctaccacgc caacgcagaa acccgcgaca tcgtaatcct caccgtgata
     8041 catccccggc atttctgccg tttcgccacc caccagtgaa cagcccgatt gcagacatcc
     8101 ttccgcgata ccgctgatca ccgctgaagc ggtatcaaca tccagttttc cggttgcgta
     8161 atagtcgagg aaaaacagcg gctctgcacc ttgcaccacc aggtcattaa cgcacatggc
     8221 gaccagatca ataccaatgg tgtcgtgacg ttttaagtcc atcgccagac gcagcttggt
     8281 acctacgccg tcagtaccgg aaaccagcac aggttcacga tatttttgcg gcaatgcaca
     8341 cagcgcaccg aagccgccca gaccgcccat cacttccgga cgacgcgttt tcttcactac
     8401 gcctttgatt cttccaacca gagcattacc cgcgtcaata tcaacaccgg catctttgta
     8461 gctaagagag gttttatcgg tcactgcttg ggtccccacg cgttacttgc ggtagaaaaa
     8521 taaaattcgg cgcaattcta acagggaaag caaacgtttg cgagactgct ttacacaacc
     8581 tttttgcacg tcttttgccc aggcacgcgg cgaaagaaga cttgtgccag ggtaaaggtt
     8641 agttttcgga tggaataatc ttctttcata accatttgaa tataaaataa ctttatctca
     8701 aaccgttatc attttgacta aagtcaacga aaagaatatt gccgccttga agaaaggagg
     8761 tataatccgt cgattttttt tgtggctgcc cctcaaagga gaaagagtat gaagatcgtg
     8821 gaagtcaaac acccactcgt caaacacaag ctgggactga tgcgtgagca agatatcagc
     8881 accaagcgct ttcgcgaact cgcttccgaa gtgggtagcc tgctgactta cgaagcgacc
     8941 gccgacctcg aaacggaaaa agtaactatc gaaggctgga acggcccggt agaaatcgac
     9001 cagatcaaag gtaagaaaat taccgttgtg ccaattctgc gtgcgggtct tggtatgatg
     9061 gacggtgtgc tggaaaacgt tccgagcgcg cgcatcagcg ttgtcggtat gtaccgtaat
     9121 gaagaaacgc tggagccggt accgtacttc cagaaactgg tttctaacat cgatgagcgt
     9181 atggcgctga tcgttgaccc aatgctggca accggtggtt ccgttatcgc gaccatcgac
     9241 ctgctgaaaa aagcgggctg cagcagcatc aaagttctgg tgctggtcgc tgcgccagaa
     9301 ggtatcgctg cgctggaaaa agcgcacccg gacgtcgaac tgtataccgc atcgattgat
     9361 cagggactga acgagcacgg atacattatt ccgggcctcg gcgatgccgg tgacaaaatc
     9421 tttggtacga aataaagaat aaaaataatt aaagccgact ttaagagtcg gctttttttt
     9481 gagtaaagcg cctataacac ataatacaga ggataatact atgacgcgcc gtgctatcgg
     9541 ggtgagtgaa agaccgccac ttttacagac aatcccgctt agtttgcaac atttgttcgc
     9601 catgtttggt gcaaccgtcc tggtgcccgt cttatttcat attaacccgg cgactgtact
     9661 gttatttaac ggtattggaa cgctgctgta tctcttcatc tgtaaaggga aaattccggc
     9721 ttatcttggt tccagctttg cctttatttc accggtattg ttactgttgc cgttagggta
     9781 tgaagtcgcg ctgggcggct ttattatgtg cggcgtgctg ttctgcctgg tttcttttat
     9841 cgtgaagaaa gcggggaccg gctggctgga cgtgctgttt ccacctgcgg caatgggcgc
     9901 aatcgttgcc gtcatcggtc tggagctggc gggcgtagct gccggtatgg cgggtttact
     9961 cccggctgaa gggcaaacgc cagactccaa aaccatcatc atctctatta ccaccctggc
    10021 ggtcacggtt ttaggttccg tgctgtttcg tggtttcctg gcaattatcc cgattttaat
    10081 tggcgtgctg gtgggctacg cgctctcttt cgcaatggga attgtcgata ccacgccgat
    10141 tattaatgct cactggtttg cgctgccaac cctctatacg ccgcgcttcg agtggtttgc
    10201 cattctgact attctgccag cggcgttagt ggttattgcc gaacacgtag ggcacctggt
    10261 agtaacggct aatatcgtca aaaaagatct gctgcgcgat ccaggtctgc accgttcgat
    10321 gtttgctaat ggcttgtcga ccgtgatctc cggcttcttt ggctctacgc caaataccac
    10381 ttacggagaa aacattggcg tgatggcgat cacccgtgtt tacagtacct gggttatcgg
    10441 cggggcggcg attttcgcta tcctgctttc ctgcgtcggt aaactggctg ccgctatcca
    10501 gatgatccca ttgccggtga tgggcggcgt ttcgctgctg ctttatggtg tcatcggtgc
    10561 ttccggtatt cgtgttttga tcgaatcgaa agtggattac aacaaagcac agaacctgat
    10621 cctgacttcc gtgattttga tcatcggcgt cagtggcgcg aaggtaaaca tcggcgcggc
    10681 ggagctgaaa ggtatggcgc tggcgaccat cgtcggtatc ggcttaagtt tgattttcaa
    10741 actgatcagt gtgctgcgcc ctgaagaagt ggtgctggac gcagaagacg cggatataac
    10801 agacaaataa tgtaccgacc gggcagtgtt gctgcccggt tcaaacatca tgggattctg
    10861 tggtaaactt ctcgcgattt tgtgaaatcc tggttgaggt atctctgaac acaccggcac
    10921 agctctcttt gccactttat cttcctgacg acgaaacctt tgcaagtttc tggccggggg
    10981 ataactcctc tttactggcc gcgctgcaaa acgtgctgcg tcaggaacat agcggttaca
    11041 tctatctctg ggcacgcgaa ggcgcggggc gcagccatct gctgcacgcg gcttgcgcgg
    11101 aattgtcgca gcgtggcgat gcggtgggct atgtcccgct ggataaacgc acctggtttg
    11161 ttccggaagt gctcgacggt atggagcatt tgtcactggt ctgtatcgac aacattgagt
    11221 gtattgccgg cgatgagttg tgggagatgg cgattttcga tctctacaat cgaattctgg
    11281 aatcgggcaa aacacggttg ttgatcaccg gcgatcgtcc accgcggcag ttgaatctgg
    11341 gattaccgga tctcgcgtcg cgactcgact gggggcagat ctacaaattg cagccacttt
    11401 ctgatgaaga taagttgcag gcgctacagt tacgcgcgcg tttgcgtggt tttgaactgc
    11461 cggaagatgt ggggcgtttc ttgctgaagc ggctcgacag agaaatgcgc acgctattta
    11521 tgacgttgga tcagttggat cgtgcgtcga ttaccgcgca acgtaagctg accattccgt
    11581 ttgtgaaaga aattctgaag ttgtagataa ggtgtttatt gtcggatgcg gcgcgaacgc
    11641 cttatccgaa ctacgcgaag ttaatccata gtattcgtag gccggataag acgtggcaag
    11701 cgtcgcatcc ggcaatgtgc ttcaactagc gatggagcac atcttaaccc acgatttcca
    11761 gtacctgctc cggtggacga ccaatccgcg ctttgccatt cgcgaccaca atcgggcgct
    11821 ccatcagctt cggattatcc accattgcct gaatcagcgc ctcttcgcta agcgaactgt
    11881 cagccaggtt cagttcttta taaagatcct ctttctggcg catcaattca cgggcgctat
    11941 tcatccccag cattttcagc aaatcgcgca gcgttgccgc atcggcgggt gtctcaaggt
    12001 agagtaccac ttctggctcc acgccgtttt ctttcagcag gttcagcgtt tcccggctct
    12061 ttgaacagcg tgggttatgg tagattttta cctgtttggt cattgttctt cctttaatgc
    12121 gaattacatc ttggtataag gcttaaagcg ttcctgcagc tggcgcaact ggtcgatgcg
    12181 cgcatcgtaa cgcgcttgtt gcaggctgcc taatttcacc tgcgaactgg cgctactcaa
    12241 cagcgaaatg gcctgatcga gtcgtccggc gagcgcataa ccttctgctc gcgcagccag
    12301 ctcctgatcg cggttattta gcgcggcttc cgcctgtgcc agtaaatccc agccgttgct
    12361 gtcatcttta ttattaaagg tgtagcgatt cagaatattg gccgcttctt gtggttgacc
    12421 gccttgtaga taagcgttcg ccaggttgag ctgcaacacc ggattggtgc gcaaatcacg
    12481 ggcatttttc aggcgattga ttgcctcatt ggctttgttt tgcccaagat cgatatcggt
    12541 agccagatcg agataccatg cgttgccagg ttctgccgcc agtaacggtt gcagcgtttt
    12601 acgcgcctcg tcgtatttat tggcttccat cgcctgtaaa gcacgaccat attgcgccgc
    12661 tcgttgctga cgaacgtttc ctttcgccca ttcatccagc aaatcactgg tgagctggtt
    12721 acgtccggaa ttatacatcc ccagtgtgcg cgctttcgcc agatagaaat cttccgacga
    12781 ctgcaccacc atcggtcgca tctgattagc acggttgcgg gcatctgcca gacgactttc
    12841 cggcaacggg tgagtcaata aaatttccgg cgggcgcgag gagtaacgcg cctgatcgag
    12901 taatttttcg aggaaggttg gcatcgcctg cggatcgaat cccgagcgtt gcagcacctg
    12961 aataccaatg cgatccgctt cctgttcatt ttgctgggtg aagctgatca tcccctggcg
    13021 cgttcccgcc agtgtaccgg tcagcgctgc catccccgcc tgcggactgg ccatcgccag
    13081 taaaatagaa cctaacgcgc cgacccaggt cagcggcgcg ctgcgctgtt gatcttccat
    13141 cgctcgcgcc aggtgacgtt gggtgacgtg ggagatttca tgcgccatta ctgaagccag
    13201 ttggctttcg ttatcggaat aacggaacag ggcagagtgc agtaccacgt tgccgccaaa
    13261 gaaagcaaag gcgttaattt cgtcgttgtt gatcagaaaa aaatgaaacg gtgtcttaac
    13321 cgaattggca tgcgaaacca gacgcattcc tagtgaatta atgtactgcg ttaatagtgg
    13381 atcattaatt aacggcgcgc tgccgcgtag ctggcggaca taatagtcgc ccatctgcat
    13441 ttcctgacca atggaaagcg tgcttcctgc ggaggttccc atatccggca aggtgtctgc
    13501 actatcggca aacgccgggg ctacctgacc aatggtcatg gcagcaatga gggttgcaac
    13561 caggtttttt ttcaactgcc tgaacataac ctctatcctg tatttctgga gatagtcatt
    13621 tgaccgatac tgtgaaatat cgttcccacc gcgccgcctt tgagtgtaac ggcgaagatt
    13681 agtccactca aatgactttt cagtttcctg aatccgtcat aaaaagcgaa gtcttttttg
    13741 tgacatttcg atacaattgc ggatcgcaat cccgctcttg aggttcaggg aaggtcttta
    13801 tgctcgaaat gttgatgcaa tggtatcgcc gccgttttag cgacccggaa gcgattgcct
    13861 tgctggttat tttagttgcc ggatttggca ttatcttttt ctttagtggc ctgcttgctc
    13921 cgctgttggt ggctattgtg ctggcctatt tgttggaatg gccaaccgtg cgcctgcaat
    13981 ctattggctg ctcccgccgc tgggcgacgt cgattgtatt ggtggttttc gtcggtatat
    14041 tgctattgat ggcgttcgtg gtactgccta tcgcctggca gcagggcatc tacttaattc
    14101 gcgatatgcc ggggatgctc aataagcttt ctgactttgc cgccacgttg ccgcgccgct
    14161 atccggcgtt gatggatgcg ggcattattg atgcaatggc cgaaaatatg cgcagtcgga
    14221 tgctgaccat gggcgattcg gtggtgaaaa tttccctcgc ctcgctggtc ggtttgctga
    14281 ccattgccgt ctatctggtg ctggtaccat tgatggtctt cttcctgctg aaagacaaag
    14341 agcagatgct gaacgccgtt cgccgggtgc tgccgcgtaa ccgtggactg gcaggacagg
    14401 tgtggaagga gatgaatcaa caaatcacca actatatccg cggcaaagtg ctggagatga
    14461 tcgtggtggg gattgccacc tggctgggat tcttactctt cgggctgaac tattcgctgc
    14521 tgctggcggt gctggtcggc ttctcggtac ttattccgta cattggcgca tttgtggtga
    14581 ccattccggt ggttggcgtg gcgctattcc agtttggtgc aggcacggaa ttctggagct
    14641 gcttcgcggt gtatctgatt attcaggcgc tggacggcaa cctgttagta ccggtgttgt
    14701 tctccgaagc ggttaacctg catccgctgg tgattatttt atcggtggtg atcttcggtg
    14761 gtttgtgggg attctggggc gtattcttcg ccattccgtt ggcgacgctg atcaaagccg
    14821 tgatccacgc ctggcccgat gggcaaatcg cgcaagaata atcttcagcc cgacgaactg
    14881 tttcgccggg ctgatttttc agggttcctg acgtaaataa atagcccgat aacaaatact
    14941 caccagcact gcaccgccga taatattacc cagcatcacc gggagcagat tggcggtaat
    15001 aaaatggctg accgtcagtg ccggaaaatt gtctgcgcta ctgtgcgcca gttgccagaa
    15061 ggaggtaggg gcgaaatggc gaatggcaat ggcgaacgga atcacaaaca aattggcgat
    15121 acagtgctca aagccactgg cgacaaacag ggtgatgggc aaaatcatgg cgacgatttt
    15181 gtcgcataac gaacgcccgc aataactcat ccacagcgcc aggcaaacca tcagattgca
    15241 cataatgccg aggctgacag attcagtaaa tgtatgatgc attttgccct cggcgcagtg
    15301 taaaaccgcg actccccaca tcgcgttttc actcatcacc agcccggaaa accagattaa
    15361 caaactgaaa caggcaatac ctgccagatt accgcaggcc accagaagtg cgttaatcag
    15421 ccaagttcgc caactaataa cgccccgact ttttgccatt accgtcatta ccgacgaggt
    15481 gaacaggttg gtgccgcaaa ccgccagcaa aataaagcca agtgtaaagc aaaggccgcc
    15541 caccagatgg gttaatgcct gtgaagacgg ggcatcggcg ataactgaaa ggtaaaaagt
    15601 aaatccgatc gccataaaca cgccagcgga catcgccatt aaaaagaccg acactttact
    15661 gcgggcaatt ttatgggcgg caatccgttc agcgattgcc gcttttctgg cgctcaactg
    15721 caagtcgaaa gagagtttgt ttctcatgca tactcctgca aaagcagatt acaacacctc
    15781 gcgaaccgag attcccagac gctgcattcg tgacagcagc gtggtgcgct tcatccctaa
    15841 tcgtgtcgcc gctccacggg gcccggcaac aatgccattg gtttctcgca atacctgaac
    15901 aatgcgctga cgttcttctt cgtcattctc tggcgtcgtc gggtgcagca actgcgccat
    15961 tgaactgcga agcgctgaat cttcatttag cgtcggcagt aaacggcttt gtcggacatt
    16021 aagatgtaaa ttcagactgt taccgcgagt cagcagtacc gctcgctcaa tcacgttttc
    16081 cagctcgcgc acgttgcccg gccagtccca cgacatcaac tggcgtagcg cctcggtcgg
    16141 gatggcgtca attgaacgat tcatatggcg cgccattttt tgcgtgaaat gctttgctaa
    16201 aagagggata tcttccggac ggtcgcgcag cggcggcaat tccagtggga agacattcag
    16261 gcgataaaag agatcgctgc gaaactggcg atcttcaacc atttgccaca aatcacggtt
    16321 ggtggcggca atgactctga catttaccgg gatcgttcta ctcccgccga gacgctcaat
    16381 ctcccgttcc tgcaatacgc gcagcagttt aggctgaagt tctaacggca gatcgccaat
    16441 ttcatcgaga aacaacgtgc cgccatcggc aatttcaaaa cgaccacgat gtgtattaat
    16501 cgcaccagta aacgcccctt tgtcatgacc gaataactca ctttccagaa gactggcggg
    16561 gatggcagcg cagttgattt tgaccagcgg cttgtcgcgt cgcgggctaa gttgatggat
    16621 cgctctggcg atcacctctt tgccggttcc ggtttcaccg caaatcaaca ccgtactgtc
    16681 gctcttcgcc acaatatcta cctgctggag caggtcttcc attgcctggc tttgatagat
    16741 aatgtcaccg acgcccagat tcgaaaggag ctgctcgcta agctggtggt tttcttgctg
    16801 caaactttcc tgcaaatcgg tcatgctgcg ccaggcatcg gcattgccaa cggcaatagc
    16861 gatgcgatcg gctatgtgtt gtagtagctg gcagttttcc tcactaaaga gagtggaaga
    16921 ggtatgcgcc agcaacaatg cacccggtgt atggttgcca aaggtaagcg gtatcaggag
    16981 cgcagattcg cagccgttag atgcaagtaa gagtaataac gggtcgcgtt gccagagaaa
    17041 cagatcatct gcacgatgcg tcaaggtcgg ttgtcgggtt tgtagcgttt gtgtcagcaa
    17101 tacactgtcg ccaggcatat tgcgtggcag acacgcacaa tgtgaggcag aaagatcgct
    17161 gcaccacagg ctgaacttct cgttctttcg atgatcgccc agtaccatac tgactgaagc
    17221 cagaccgaaa aaatgatgga tctcacgggc gacgtcagcg atcagatcgt cgagatcaag
    17281 atgtgacagc actgcattag tgatatccac cagtacccga tgatgatcgc gatctttctc
    17341 cagcaaacgc agcgtcacct gctgttcttc atcattgagc catgattgca gaaccggggt
    17401 gataatgcgt gccagccagt taaacagtgt gcaatcctgg ggagtaaaac ggcagccgtg
    17461 ggtacgaata aagcgtagct ccgccattcc actactgccg agaaggacgg cgtgacgcga
    17521 ataatcgacc atctcgccct gatactctgt tgttgccgtc acatcgctgc catcatcatg
    17581 gtagcggtaa tagaagttgc ttttgagaac atgcagttca ataagttgta ccgggctgaa
    17641 cggcggcggc agcgggatga aggcgcgtaa taaagacgcc ttaccgtgtt tctgcgctaa
    17701 ccgctccgcg agcattccgt ttaccgcttc aattgttatt ccttgcggcg gggcaaacat
    17761 cgcctcgtct gacatagcca tcaacagtcc ttattaatga gaaatccttc tcacgcaatt
    17821 tttctggcca tcagatagat ggccggttcc tgcacaattt catccaacag atgaatgaat
    17881 tgaatgctac gcgcctgcca cacagacgtc gcgtcatccg ccagtggcgc gaatgcccgg
    17941 gtcaacaggg cggtgtggct ggcgtcaata acaatctcac caaaagtcat cacccccagc
    18001 atcttacgtc gggcttgctc ctcttcgacc tgggcaagcc aatcttcgta ttccgtcagt
    18061 gggcagggga aggcgacatt cagacaatca atcacgccaa cgtgatgacc gatagccagc
    18121 gagtaataca ttacctgaga gctgtgttcc ggcatctcgt cgctactggc gacaaacttt
    18181 tttcgcagcg tccagaaaac aatttccccg cactcttcag tcatgtaacc cccggagtac
    18241 gcgaaagagt tgctgaacga tactattgag acgtggatcg tcggcatcgc gcaaccaggt
    18301 gttaacccga tttcctgtag gatcgtcgct taaatggcgc aggagccgat cgcaaatttc
    18361 tcgcccctga cgatagcccg ccagccgccg cgcttctcgc tcaatggcga tacgctgtga
    18421 tggcgggatc tgtggccaca acggttgcat agtcaccccg gtgggatcgc gataatccac
    18481 agcgtgaatc ttctgttgca gcaaaccgag cgccacggcg aaaccgtgaa tggtggcggc
    18541 cggtgttggc gggcagccgg ggatccaaac atcaatgggg acaatggtgt cgctgccgcc
    18601 ccagacgctg tagagatcgt ggaaaatacc gccgccgaca ccgcacgcgc cgtaggaaac
    18661 acagatttta tgatcggggg cagactcata cgcccgaagt gcaggcatac gcatcgcccg
    18721 ggtgactgcg ccagtaaata acaaaatatc ggcgtgacgc ggtgatgaaa caaccttaat
    18781 gccaaaacgt tctgcgtcga atactggtgt gatggcagca aaaatttcga tttcacaggc
    18841 gttgcagccg ccgcaatcga cgcgataaac gtaagccgag cgacggatat cctgtagcaa
    18901 atgctgcttc atcttttgcg tttgctcgtc cagcgtgatg ggctggctga catattgcgt
    18961 aagcactgga ctcatagctg ctccttagcc accagcggta catctgtatc gtcgttgatc
    19021 agcgtcgcgc gttgtttgca ctccgggcag acgctcgctt gcgcccgcaa catttcgcgg
    19081 ttttgtggcg cattttgttg ctgtgccaac aattcagcag ccagtgcgac ggttttttgc
    19141 ggggcaaacg ggcgttcgca acggctgcaa cgttgtagat ggaacgtcgc gcgggtatag
    19201 agatcggctt tattggtgac ggtcagttca aagttattgg taagctggat ggctctggtc
    19261 gggcacactt cttcacaacg tccgcagtaa atacaacgcc tcagatagag ctgccaggtg
    19321 cgcgaatttt gctggtcgtc ggtctggata gtcagcgcat ttgccggaca agcacaggcg
    19381 ccgcaggcaa tacattggct gggcatcagg tccggtttcc cgcgaaagcc tgggctgacc
    19441 tccagtggcg cgaagggata tttcaccgtc gcggttccgg cgcgcataat agttttcagt
    19501 aacttcagca tcttctgtcc ttacttcagc ggcgaacggt tacgatcaat gccgtagcgt
    19561 tcgatctctt tatacggcac ggtttttgac tggcgcttgc gcacatctac cagcgtcaca
    19621 cggtcggtac aggagtagca gggatcaagg ctaccgataa tcagcggtgc gtcagaaacg
    19681 gtattgccgc gcagcatgta acgcaacacc ggccagttgg cgtaggtggc ggcacgacag
    19741 cgccagcgga acaatttttg gttatcaccg agcatgctcc agtgcacgtc ttcaccgcgt
    19801 ggcgcttcaa caaagcccag cgcgaatgcg tgaggtttat agctaaagcc ttcggtcagc
    19861 agcggggtat ccggcatgtt gtcgagggca aattccagca ttgccagcga atcaaacgtc
    19921 tctttgacac ggaccatcac gcgggagaaa acatcgccgc cggtgaaggt aaacagtgtt
    19981 tttggaatat tgccgtagtc ggcgtagggg tgatcaaagc gcaaatcacg ggcaaaacca
    20041 ctgccgcgga tcagcggccc tacagggcta taatcacggg cgatttgtcg gtcgagaatg
    20101 ccaatgccct gagtgcgttg ttccatattc ggcatagcaa gcagcatctc taccagctcc
    20161 gacacgtcgg cgcgcatctc gtgcaccagt ttcagcgttt gcagacgttg ctctttgaga
    20221 atatcgcggc gaacaccacc aatcagattc agaccgtagg ttttacgcga cccggtcagc
    20281 aattccgcca tcgtcatcga cttttcccac acgcggaaaa attgcataaa gccggtatca
    20341 aaaccaacaa aatggcagga gaggccaagg ttgagcaaat gactgtgtag ccgttcgact
    20401 tccagcagaa tcgagcgaat ggtatgcgct cgttgcggca cctcaatccc cagtgcattt
    20461 tcaaccgagt tggtataggc cacactgtgg gcaaaaccgc aaatcccaca cacgcggtcc
    20521 gataagaagg tcacttcgtt gtagcccatc cgcgtttctg ccagtttctc catgccgcga
    20581 tggacataaa acaggcggta atcagcatcg acaatttgct cgccatccac aaacaagcgg
    20641 aagtgacccg gttcatcgga ggtgatatgc agcgggccga cagggatcac ccgcgcatcg
    20701 ctgttgccct cattgataaa cgcatacgtt tcggaatctg tcgtcggttc agggcgcagt
    20761 cgataatcca tcgcatcttt gcgcagcgga tgcatatctt ccggccagtc atcgggcaac
    20821 accaggcgac gctgatccgg caggccaacc ggaatcagcc cgtacatatc gcgaatttct
    20881 cgctcgcccc agaccgcggc agggacgcgc ggtgtgactg acggaaactc ccgactgtcg
    20941 gcatcgacca gtgccttcac cacgatccag catttttcgg ccccttccat tgaaagggca
    21001 taataaaccg cgtaatgacc gttaagtgtc cgctcgtcgt tgccaaacag gactggaagc
    21061 cagccatcat gttgataata aagataatgt acaacgtcag gcagcagatt gattttcacc
    21121 gtaatggtga cctgttcagg cgtttgtcgc tcttcatcca gcaccgcgcc ggggaactgc
    21181 gttttcaggg cggcgagaat cgcttcgcca cgatttgacg atgaattaac gttcactgtg
    21241 tttctccgaa acagatgcgg tgcctgaagg caaaaaatca tgccaggtgc tacgttgtgc
    21301 aggcaggtcg tgcgtccctg agaggactat agtggaagcg cccgccagga tcctgatgac
    21361 aggttgtgga atatgcgttc ccatcgccag catcatgacc agcagaatca ccattggcga
    21421 ggtggtcaac cagccgagat caccccggtt aacggcctgc ggcggtttcg ccattaacac
    21481 ccgcgcagcc atccgtacca ggcccgccag caccagcgtt aacagcaata acagcaggac
    21541 gataatcagc aggtgattac gtgccagtcc tgcggtaacg gtcataaatt cgctaagaaa
    21601 aatgttgaag ggcggcatcc ctgccagcgc cagcgcaccg ccgccaaaca gcacggcggt
    21661 aaatggcatg attttgagca tcccacagac gacgttgaga tcgcgcgtgc cgtacttgag
    21721 cagtacattg ccggaaccgc agaacagcag cgtttttgcc agactgtggt ttaaggtgtg
    21781 cagcagcgcg gcaaaaattc ccagcggccc gccaatgcct agcgccaccg cgaccagccc
    21841 catgttctcc acgctggagt acgccagcag acgcttaatg tcccgctgta ccagaatgaa
    21901 aaatgccgcc acggcaaccg acaacatgcc gaagatgagc aacaaccggt tggggaaatc
    21961 gctgccgatg gcctggcaga taatgatgta atagcgaatt agcaccaaca gcgcgcagtt
    22021 cagcaatacg gcggagagca gggcgctgac cggactcggt gcttcactgt gagcatccgg
    22081 cagccaggcg tgcatgggaa atagcccggt tttggtgcca aagccaatta gcacaaacac
    22141 aaaggccaac agcattaatg ttgggtcaag caaggacgat tgcttaagaa cctcgctcca
    22201 gaatatcgcc atttctgcct gcggcataac gctggcgacg ttggcgtata ccagcacggt
    22261 accgaacaga ccaaaagcga caccaacagt acaaataatg atgtacttcc atgctgcttc
    22321 cagcgatgaa cgctgaccgt aaatgcctac cagaaacgcc gagcttaagg tggtggcttc
    22381 gatcgccgcc cacatcacaa tcaggttatt gctggtaaca accagcagca tggtaaacaa
    22441 aaacagatgg aagaaaccgt agtaatcgca cagcgtaacg ggggaaagct cgccgtgtgc
    22501 cacttcatga cgcatgtagc caatcgagta aacaccggtg agaaagccaa tcacaccaag
    22561 aatggcgagg aacaaaccgc ccagaccatc aatatgaagc cacagtcccg cagcgaatat
    22621 ttctcctgca tcagcggcag tttggaccac ccagagtgcc agaatcagca gcagtgtgat
    22681 ccctaagcta tgtaatacag tcaccgtaca agtcgcagag agcccccgtt tccggcaggc
    22741 aaaacagagc agcgaaaaaa gcagcggcgt gagcaggagt aaagcgaaca tcacagaata
    22801 actcatctca ttatcccttc agcgcggtca agttgttcac gtccagcgtg ccgtgggtac
    22861 gccatatttt tcttgccagt aacaccatca caatgacggc gaagatggcg tcggtagcga
    22921 tacctatttc caccagttcc ggtgctcgcc aggcaagaag cgccagcacc agatgggagc
    22981 cgttttccat caggcagtaa ccaaaaattt gccgcaggat attgcgctgg ctgacaatgc
    23041 acagcaggcc aagcagaaaa tgacctaacg ctaccgccag cgccggtttc agcccggtag
    23101 ccatcggtag cttcacgggc tgaacgacaa atgcgcaaag caggacaatt aacgccgcga
    23161 gcagtgccat cattgccgga ccgaataacg ctttttccgg gatgttctgg ggaatatttc
    23221 gtgcagcgta agtcatgatt aacggtacca gcagcacttt ggtgataaag gcgctggcgg
    23281 accagatcag cagttgctct gcggcgaaca ggcacgagag agtggcgaaa atagacacca
    23341 gcaccagcga ctggcaggcg taaaatccgc atgacaggcg atagcttttg acgctaatca
    23401 caaacagcga tgtcagcatc atcagtcccg ccagattatt tacgatcata gaaccggtca
    23461 ttcatcatct ccttactgca accaggtcgc ggcgattacg ctggaaatca gcgacatcac
    23521 aatcagtaca atcaacacca ggcgcattga ccctggcagc ggtgcggcat cggcgacggc
    23581 ctcgctcggt ttgccaggca caacgcgacc aaaccagcga ataaaccagg cgaaactggc
    23641 gaccgattca atcatcagca gaatcatggc gggcagcagg atccagtact ccactgacaa
    23701 cgcaaaaccg gcagcaaaca gcgggaattt actgaagaag ccgttgaacg gcggcacgcc
    23761 ggtaatcgcc agcgctgcca cgcagaaacc cacgcctggc aacggcaggg tgtgcaatac
    23821 gccacgcaga cgcggcaaca agcgcgtgcc gcagctgtaa ctcagcgcac ctgctacaag
    23881 gaaaaacagg cttttagcga acgcgtggtt gacgatgtag gcgatgctac cctccagcgc
    23941 cagccgcgag ccgaagatgg agagcgacaa gccgaagaac atccagccaa gttgagtgat
    24001 ggtcgaccag gccagcaacc gcttcatatc ctgctgtggc aaatacatca gaaagccata
    24061 aagaatggtg accagtgcca tgaccatgcc aacgccgcca atcacatgcg ggatattgcc
    24121 gccgtcgata attgcgcggg caaaaatgta aacgcccact ttcaccatcg atgcggcgtg
    24181 gagataggcg ctgatcggtg ttggcgcttc cattgcatcc ggtagccacg cttgcatcgg
    24241 tagctgggcc gatttccccc acgcggcaaa caggatcccg ccataaacca gataacgtgc
    24301 gtcgccgtgt aactcgctca ttgcgctaag cgcaaacgtt ccggtctgca aaaacagcgt
    24361 ggcggcggca agatacaacc ccaacgaacc gatatgggtg ataagcaacg cttttagtgc
    24421 tgaacgctgc gctttatcgc tctggtaata actgatcaac gcccaggagc agccgcccta
    24481 atttcaaaaa acaacaactg accgagcagc gtcgaggaga gtaccagtcc cgccatcgcg
    24541 ccgataaaca ccagcaaaaa tgcgtaataa cgattcgtgc cgttatgcgg gtgttcgcga
    24601 tttttatccg tcagataacc cgtggagtag atcgtgacca gcaaaccgag aaacaccacc
    24661 acaaacagaa tcagcgtact cacgcgatca atgaccaggc caaacaatgc cacatccccc
    24721 acgttgacca acgtcaacgt gacggcaact ttatcggcct gataaaacgc ggagatcagc
    24781 gacaacatgc acagcgtggt cagtgcggcg aacaaaaccc ccattcggcg gcccgacgtt
    24841 gtggcgaaaa cgaaacgacc agtgcgccga taaaagacag cattaacgtc gtcagagcaa
    24901 gattttccat attccgtcag tgctccttac agaccggtta accagaagac ccatgcaagc
    24961 gcagcaaggc tgaagccaag ccaggtcaca tggtgaatga gtaaaaaacg cccgcgtgcc
    25021 agcgtgtttt ctgcgattga ggccagtaca aaaatcagca aaaccttgag cagcgtaacg
    25081 acaagtgaag tcagcaggca ggcgagagaa agttcttgcg cgcgcccaaa gggcagaaac
    25141 agggccacaa acagtgctgc catcacgacc tgtttcagcc ccagccccca tttcgctagc
    25201 gccagcccgg caccggaata ttcggtcagc gggccttcct gtaattcctg ttctgcttca
    25261 gcaacatcaa agggaatttt tcccatctca atgaagcagg caaaaccaca ggccagtaac
    25321 gccagtacgg tggttagcgg cgagttccag cccgttgcca gcgtattgct gatcatctcg
    25381 atatgcgtgg aacctgctat cagcgccaat accagcagtg agagaatgag catgggttcg
    25441 accagaatgc cgagcgtcaa ctcgcgactg gcaccgactc ccgcaaacgg gcttccggta
    25501 tccagcccgg aaagagcaaa gaaaaaacga aacagggcaa gaagatagat aagggtgatc
    25561 agatcgccgc cgcccgcaaa aggggaaacg gtaataaaaa gtggtagggc catcgccagc
    25621 accagcatgc tgctgattaa tacccacggc atcaggcgga acatcagacc tgaagatata
    25681 ggcgcaactt cctggcgttt aaacagtttg tggatatcgc gataatcctg ccagatcccc
    25741 ggtccgcggc gggagtgcat tcgcgcgcgt atctgccggg aaatacccgt aaaaagtggg
    25801 gttagcatca gcagaatcac ggcctgtgct aacgcaaaaa tgaccagata tccgtcgcaa
    25861 agagtttgtc tcatggtgat ttccttagac ggcaatagcg attagcagca caaccagtgc
    25921 ggcaaccaca tacaggcaat agagacgaaa gtcgccgctt tgcagatgct gtatttcttt
    25981 ggccagccgt tgggtggcgc tcacgatggg gcggatcacc cgctcatccc agaagggttc
    26041 tgtgctctga gccctggcgg tgacgtgcgc aagaccttta tttagcctca gcgtagggtc
    26101 gagctgttgt cgtagacgaa atagcgcaga aaagaccaca cgcagcggct gcatcacgcc
    26161 attgcctgac ggggccatcg cattttccca gccgtagccg catgcccagg catctcctgt
    26221 gcgacgaaac gctgcgcggc gcgaacgaca ccacagccag taaaggccag gcagcaaagg
    26281 catcgccagt agtaacagca acagtaatga gggggtgacc cgtgtatgaa acgtgccggg
    26341 tacaagtgct attccgctgg cgacagtgac gggaggggta ttggtaaacg cataggcaat
    26401 atgcattatc ttcggtgcca gccaactggc actaagcgca attaatacgc agagtgccgc
    26461 gagcagtagc atggcgacga tcatcgtatt tggcacttcc tgtgcctctt cagcgtgtgt
    26521 actgcgcggc gcaccacaga aagtaatacc gtacattttg acgaagcaca ttactgccag
    26581 cccaccagtg actgccagca ttaccatagc aataggaccc gcaagttgta gcgctacggc
    26641 ttccacacga cttagtgaga acagcgactg ccaggtgtac cattcgctga taaaaccatt
    26701 cagcggagga atggctgata tcgcgaggca accaatcagg catgctgcgg ctgtccacgg
    26761 catccgtttc gccagtgccc ccattttttc catgtcgtgg gtatgcaaac gcgaaataat
    26821 cgcacccgcg ccgagaaata gcagcccttt gaacagcgca tggttgagca gatgaaacag
    26881 tgcgccgagc agtccaacca cggtgagcag cgggtcgtgc agtgacagac cgtccatcgc
    26941 cacaccgacc gccagcaaaa taatgccgac gttttcgacg gtactccagg ccagcagccg
    27001 tttgatatcc tgttccgcca gtgcatacag cacgcccagg agcgcggaga ttgcgccgac
    27061 cgccatcacc agagtgcccc accacagagg caaacccgtt tgcgccagca gatccatcgc
    27121 tactttcagg atgccgaaaa taccgatttt gaccattacg ccagacatca acgccgaagc
    27181 gtgcgatggt gcggcagggt gagcgcgcgg caaccagctg tgcaacggca tcatcccggc
    27241 tttcgcgcca aaaccgaaaa aggccagcag aaacaccgcc gacgccagcc ccggagaaag
    27301 tgaaagcgtg cgaaactggc aaaatcgagg ctgccgcttt cgcgccccat cagcaagaag
    27361 gcgatcataa tcagcaccga tccggcgtgg gcgataaaaa agtagagcat gccagcgttg
    27421 atcgacgttt tatcctgcct ggcaatgacc agaaaccagg aagacagcga catcatttca
    27481 aacagcacga tgaaccaaaa agcgttgtcc atcaccagca gggcaaccat cgatgcgatg
    27541 aaaagattca taaagaagcc catcgccgcc gcgcctttgc cctcgtattc gcgcatataa
    27601 gtcaatgaat agagcgaaca aaccaccacc agcaaggaga taaccagtac cataaatgcc
    27661 gagaggctat ccattcgcag ggttaaatcg gcaaagtcaa aaggggtggc aaaatgggcg
    27721 actaaagtta caccagcatg taattgcgta attccgctga tgatgccaat cacgccgccc
    27781 accagcgatg tgatgccgga aagcttaata gccagtctgt ccagaccgag taaaaacagc
    27841 gaagccagac tggcaaacag atagaggatc agcgaccagg ttaataattg caaggcatcc
    27901 attagtgttg ctcctgagtg agggaaaggg gatcctcccc gccattggca aaccagcttg
    27961 cggcaaggcg ctgtttttct ttcatctgac gttgcaggct atcgtcggtg atcagccgta
    28021 acgcctgatt cgggcaagcg cgaacgcagg ccggaccttc tggcaagaaa tcacacaggt
    28081 cgcatttcac cgcgacggtc tggacacccg gttcccggcg cagcaacgca tgttggggca
    28141 gcacgttttc ttcgccgtct ttgagtgagc cttcagcctg aaaaacatat tgcgcatggg
    28201 cattcaccgg acggcttcct gaagcgctga ttgcgccaaa tgggcagacc acggcgcaaa
    28261 gcttgcagcc aatacagagg ctttcgttga gttggatcgc atcatccctc tgggagatgg
    28321 cattaaccgg gcagacctgc aggcaagggg cttcctcaca gtgatgacac acgacagggg
    28381 cagtgagtgt tgacgtcttc gccagggcca ggcgcgggtg ttgctgtaaa ccttgcgttt
    28441 tatgcacgtc cgaacaggca gcgagacagg tattacatcc tgtacaccac agtggttcgg
    28501 ccaccacaaa gcggttcatg tcggctcctt gaaaatgata tgaaacattg catttcatat
    28561 tgcataaagg atgccattct gattaactca atatattcaa taagttatgt attgactgga
    28621 ttctgaacgg tacaaaaatg atggtcatta acgttcagca aaaaatggat tcgacacccg
    28681 tgtcgcaggg gaaagcgaga atgtgatctt gatcacgaga agtgaggtat gctggccgca
    28741 agcgcagcca gcacggaatg gagcaaagta atcaggcgtg ttctttcagc cagttcagca
    28801 caacgtcgtg gtgattgctg gttttgaaat cgtcaaagac atgttcgatt ttgccatcag
    28861 cgtcaatcag gaagctgatg cgatgaatgc catcgtaggt tttgcccatg aaggactttt
    28921 caccccagac gccgaattgt tcgcacacct ggtggtcctc atcagacagg agcgtaaagt
    28981 taagcagctc tttttccgca aaacgggaga gtttttcggg tttatcggtg ctgataccca
    29041 gcacatcaac gcccgctttt ttcaactcat ccatgttatc gcgtaagccg caggcctgta
    29101 cggtacagcc gggggtcatg gctttcgggt agaaataaac cagaacacgc tgtccctgga
    29161 agtcggtcaa attaacttgt tctccgtctt gatccggcaa gctaaatttc ggtgcgatat
    29221 caccggcttt cagtggattc attacttaac tccatcctgt tcatcatgtt gggaataatt
    29281 gacgacgtta atactgcctt gtgcattgag ttctgtacat agggctttga acgcttgctc
    29341 aatatttgct gcgtccgcag atgcggggct gtgggcggtt atctgaatat gcaactgcgc
    29401 agcccgttca ttttcagcag gttgcgtgcg cgacaccagc tccgcaatgt tcatatgatg
    29461 cgcgtcgaaa agtgctgtga agcgttcaat taaatgcggg gagtctgcca catcgacctg
    29521 aacccagaca gatgctggca ttggcggacg cggacgcgcc gtcgtgcgct tcatcacgat
    29581 taaaagatcc agttcggcac ctttcaacgg taacgttgat tcaatcagag taatggcatt
    29641 ccatgaaccg gaaagcagca taataaacgt gaactcttct cccagcatcg ccaggcgact
    29701 gtcttcaata ttacagccgc aactactgac atgacgggtg atggtgttca caattccagg
    29761 gcgatcggca cccaacgcag tgatcaccag ataatgttgc gatgacagtg tcaaactggt
    29821 tattccttta aggggtgagt tgttcttaag gaaagcataa aaaaaacatg catacaacaa
    29881 tcagaacggt tctgtctgct tgcttttaat gccataccaa acgtaccatt gagacacttg
    29941 tttgcacaga ggatggccca tgttcacggg aagtattgtc gcgattgtta ctccgatgga
    30001 tgaaaaaggt aatgtctgtc gggctagctt gaaaaaactg attgattatc atgtcgccag
    30061 cggtacttcg gcgatcgttt ctgttggcac cactggcgag tccgctacct taaatcatga
    30121 cgaacatgct gatgtggtga tgatgacgct ggagctggct gatgggcgca ttccggtaat
    30181 tgccgggacc ggcgctaacg ctactgcgga agccattagc ctgacgcagc gcttcaatga
    30241 cagtggtatc gtcggctgcc tgacggtaac cccttactac aatcgtccgt cgcaagaagg
    30301 tttgtatcag catttcaaag ccatcgctga gcatactgac ctgccgcaaa ttctgtataa
    30361 tgtgccgtcc cgtactggct gcgatctgct cccggaaacg gtgggccgtc tggcgaaagt
    30421 aaaaaatatt atcggaatca aagaggcaac agggaactta acgcgtgtaa accagatcaa
    30481 agagctggtt tcagatgatt ttgttctgct gagcggcgat gatgcgagcg cgctggactt
    30541 catgcaattg ggcggtcatg gggttatttc cgttacggct aacgtcgcag cgcgtgatat
    30601 ggcccagatg tgcaaactgg cagcagaagg gcattttgcc gaggcacgcg ttattaatca
    30661 gcgtctgatg ccattacaca acaaactatt tgtcgaaccc aatccaatcc cggtgaaatg
    30721 ggcatgtaag gaactgggtc ttgtggctac cgatacgctg cgcctgccaa tgacaccaat
    30781 caccgacagt ggtcgtgaga cggtcagagc ggcgcttaag catgccggtt tgctgtaaag
    30841 tttagggaga tttgatggct tactctgttc aaaagtcgcg cctggcaaag gttgcgggtg
    30901 tttcgcttgt tttattactc gctgcctgta gttctgactc acgctataag cgtcaggtca
    30961 gtggtgatga agcctacctg gaagcggcac cgcttgcgga gcttcatgcc ccggctggaa
    31021 tgattttgcc ggtgacctcc ggtgattatg caatcccggt gaccaacggt agtggtgctg
    31081 tcggtaaggc gctggacatt cgtccaccag cccagccgct ggcactggtt tctggcgcgc
    31141 gtacccagtt cacgggcgat accgcttcat tgctggtgga aaatggtcgt ggcaatactc
    31201 tgtggccgca ggtggttagc gtgctgcagg cgaaaaacta caccatcacc caacgtgatg
    31261 atgctggtca gacactgacc accgattggg tacaatggaa ccgtctggac gaagacgagc
    31321 agtatcgtgg tcgttatcaa atctctgtta agccgcaggg ttatcagcag gcggttacgg
    31381 ttaaactgct gaacctggaa caggcgggca aaccggttgc agacgcggct tccatgcagc
    31441 gttacagcac ggagatgatg aacgttattt ccgccggtct ggataaatct gccactgacg
    31501 ccgcgaacgc tgcgcaaaat cgtgcctcca ccactatgga cgtacaaagt gcagctgatg
    31561 acaccggttt accgatgctg gtcgtacgcg ggccgttcaa tgtggtttgg caacgtctgc
    31621 cagcggcgct ggaaaaagtg ggcatgaaag tgaccgacag cacccgttcg cagggtaaca
    31681 tggccgtaac ttataagccg ctgtctgaca gcgactggca ggaactgggc gcgagcgatc
    31741 caggcctggc atccggtgac tataaactgc aggttggcga tttagataac cgcagcagcc
    31801 tacagttcat cgatccgaaa ggtcatactc tgactcagag tcagaacgac gcgctggtat
    31861 ctgtcttcca ggctgcgttt agcaagtaaa aatacagggc tggaatcatc cggccctttt
    31921 ttctgatatg atacgcaaac gtgtgcgtag gcaggaaaac gcgattttag cggtaattcg
    31981 cacgaaattt gtttgtcgga cgtagttcga ataaggcgtt cacgccgcat ccgacaaaac
    32041 atccggcaca ccagacagca aaagatttta aaacgttaat tcacacccag gagtgataaa
    32101 gatgcaaaag caagctgagt tgtatcgtgg taaagcgaaa accgtataca gcacggaaaa
    32161 cccggacctg ttggtgctcg aattccgcaa tgatacgtca gcaggggatg gcgcgcgcat
    32221 tgagcagttt gatcgcaaag gtatggtgaa caacaagttc aactacttca ttatgagcaa
    32281 actggctgaa gcgggtatcc cgactcaaat ggagcgtctg ctctccgata ccgaatgtct
    32341 ggtgaaaaag ctggatatgg tgccggttga gtgtgtcgtg cgtaaccgtg ctgctggctc
    32401 tctggtgaaa cgtcttggaa tcgaagaagg tattgagctg aacccgccgc tgttcgatct
    32461 gttcctgaaa aacgacgcca tgcacgatcc gatggtcaac gaatcttact gcgaaacctt
    32521 tggctgggtg agcaaagaga acctggcgcg tatgaaagag ctgacctaca aagcgaacga
    32581 cgtgctgaaa aaactgttcg atgatgctgg tctgattctg gtcgacttca agctggagtt
    32641 tggcctgtac aaaggtgaag tggtactggg cgatgagttc tccccggacg gcagccgcct
    32701 gtgggacaaa gaaacactgg agaaaatgga caaagaccgt ttccgccaga gcctcggtgg
    32761 cctgatcgaa gcctatgaag ccgtcgcccg ccgcctgggt gtacagctgg actgattttt
    32821 ctggttgctt gtttctttag ccggagggta ctccggctaa atgtctttga tacccccgct
    32881 acaatacttc tcattatctc cctttgacag gaaagtcata ccaggccaat atgtatatac
    32941 ataacgtata tcccaaaaag ggggggctat ggaaactact gtatttttaa gtaatcgcag
    33001 tcaggccgtc agattaccga aagcggtcgc tctgccagaa gacgttaaga aagtagaaat
    33061 tattgcgatt ggtcgtacaa gaatcatcac tcccgccggt gagtcctggg acagttggtt
    33121 tgatggcgaa aatgtgagtg cggattttat ggatatccgt gatcaacccg caatgcagga
    33181 aagggaatca ttttgatgct gaaattcatg ctggatacca atatctgtat tttcactatt
    33241 aagaataaac cagtacatgt cagagagcgg tttaacctga attctggccg gatgtgtata
    33301 agttctgtaa cattgatgga gcttatctat ggggcagaaa aaagtcagat gccagaacgc
    33361 aatttggcgg ttattgaagg tttcgtatct cgtttggtag tgcttgatta tgatagtgct
    33421 gctgcgacgc ataccgggca aatcagggcc gagcttgccc ggcagggaag gcctgttgga
    33481 ccttttgacc agatgatagc gggacatgct cgcagtcgcg gtttgattgt ggttaccaac
    33541 aatacccgag agtttgagcg agtggcggga atcagactag aagactggag ctaacattct
    33601 cttttacttc taacattcgt aaattatata acgtggcatg a
//
LOCUS       DAZFQD010000040        33137 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.040, whole genome shotgun sequence.
ACCESSION   DAZFQD010000040 DAZFQD010000000
VERSION     DAZFQD010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33137
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.040"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(72..1931)
                     /gene="gss"
                     /locus_tag="ACHKTW_001995"
     CDS             complement(72..1931)
                     /gene="gss"
                     /locus_tag="ACHKTW_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HFO6463025.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            2136..3002
                     /gene="yghU"
                     /locus_tag="ACHKTW_001996"
     CDS             2136..3002
                     /gene="yghU"
                     /locus_tag="ACHKTW_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HFO6463026.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPVSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETMNWLFWLQGAAPFLGGG
                     FGHFYHYAPMKIEYAINRFTMEAKRLLDVLDKQLAQHKFIAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(3031..3112)
                     /locus_tag="ACHKTW_001997"
                     /pseudo
     CDS             complement(3031..3112)
                     /locus_tag="ACHKTW_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3125..3373)
                     /gene="hybG"
                     /locus_tag="ACHKTW_001998"
     CDS             complement(3125..3373)
                     /gene="hybG"
                     /locus_tag="ACHKTW_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HFO6463027.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(3386..3727)
                     /gene="hypA"
                     /locus_tag="ACHKTW_001999"
     CDS             complement(3386..3727)
                     /gene="hypA"
                     /locus_tag="ACHKTW_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HFO6463028.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPICHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(3720..4208)
                     /gene="hybE"
                     /locus_tag="ACHKTW_002000"
     CDS             complement(3720..4208)
                     /gene="hybE"
                     /locus_tag="ACHKTW_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HFO6463029.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRSGE
                     NA"
     gene            complement(4201..4695)
                     /gene="hybD"
                     /locus_tag="ACHKTW_002001"
     CDS             complement(4201..4695)
                     /gene="hybD"
                     /locus_tag="ACHKTW_002001"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HFO6463030.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKSAPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(4695..6398)
                     /gene="hybC"
                     /locus_tag="ACHKTW_002002"
     CDS             complement(4695..6398)
                     /gene="hybC"
                     /locus_tag="ACHKTW_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HFO6463031.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(6395..7573)
                     /gene="hybB"
                     /locus_tag="ACHKTW_002003"
     CDS             complement(6395..7573)
                     /gene="hybB"
                     /locus_tag="ACHKTW_002003"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HFO6463032.1"
                     /translation="MSHDPKPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLQRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIVLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(7563..8549)
                     /gene="hybA"
                     /locus_tag="ACHKTW_002004"
     CDS             complement(7563..8549)
                     /gene="hybA"
                     /locus_tag="ACHKTW_002004"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HFO6463033.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(8552..9670)
                     /gene="hybO"
                     /locus_tag="ACHKTW_002005"
     CDS             complement(8552..9670)
                     /gene="hybO"
                     /locus_tag="ACHKTW_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HFO6463034.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGAAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEEGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(9859..10146)
                     /gene="yghW"
                     /locus_tag="ACHKTW_002006"
     CDS             complement(9859..10146)
                     /gene="yghW"
                     /locus_tag="ACHKTW_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 family protein YghW"
                     /protein_id="HFO6463035.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(10265..11152)
                     /gene="yghX"
                     /locus_tag="ACHKTW_002007"
     CDS             complement(10265..11152)
                     /gene="yghX"
                     /locus_tag="ACHKTW_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YghX family hydrolase"
                     /protein_id="HFO6463036.1"
                     /translation="MPRLTAKDFPQELLDYYDYYAHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIVAEYITYPSPNGHGEVRGYLVKPARMSGKTPAVV
                     VVHENRGLNPYIEDVARRVAKAGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQAAGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHFAELDTRINEGWPAYEAALKANNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            11309..12349
                     /gene="gpr"
                     /locus_tag="ACHKTW_002008"
     CDS             11309..12349
                     /gene="gpr"
                     /locus_tag="ACHKTW_002008"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HFO6463037.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMHREGNKVRGLTPKMLTEANLNNLRLLNEMAQQ
                     RGQSMAQMALSWLLKDDRVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(12389..12883)
                     /gene="yqhA"
                     /locus_tag="ACHKTW_002009"
     CDS             complement(12389..12883)
                     /gene="yqhA"
                     /locus_tag="ACHKTW_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HFO6463038.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLNWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            13074..13958
                     /gene="yghA"
                     /locus_tag="ACHKTW_002010"
     CDS             13074..13958
                     /gene="yghA"
                     /locus_tag="ACHKTW_002010"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HFO6463039.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPVPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASIITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQQTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(13997..14422)
                     /gene="exbD"
                     /locus_tag="ACHKTW_002011"
     CDS             complement(13997..14422)
                     /gene="exbD"
                     /locus_tag="ACHKTW_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HFO6463040.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(14429..15163)
                     /gene="exbB"
                     /locus_tag="ACHKTW_002012"
     CDS             complement(14429..15163)
                     /gene="exbB"
                     /locus_tag="ACHKTW_002012"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HFO6463041.1"
                     /translation="MGNNLMQTDLSVWGMYQHADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            15415..16602
                     /gene="metC"
                     /locus_tag="ACHKTW_002013"
     CDS             15415..16602
                     /gene="metC"
                     /locus_tag="ACHKTW_002013"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HFO6463042.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFDSVEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCVLFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLSNEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            16742..17401
                     /gene="yghB"
                     /locus_tag="ACHKTW_002014"
     CDS             16742..17401
                     /gene="yghB"
                     /locus_tag="ACHKTW_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YghB"
                     /protein_id="HFO6463043.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGRWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(17441..18340)
                     /gene="yqhC"
                     /locus_tag="ACHKTW_002015"
     CDS             complement(17441..18340)
                     /gene="yqhC"
                     /locus_tag="ACHKTW_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HFO6463044.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            18534..19697
                     /gene="yqhD"
                     /locus_tag="ACHKTW_002016"
     CDS             18534..19697
                     /gene="yqhD"
                     /locus_tag="ACHKTW_002016"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:1990362 - butanol dehydrogenase (NAD+)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HFO6463045.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGAGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRETKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            19802..20629
                     /gene="dkgA"
                     /locus_tag="ACHKTW_002017"
                     /pseudo
     CDS             19802..20629
                     /gene="dkgA"
                     /locus_tag="ACHKTW_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050580 - 2,5-didehydrogluconate reductase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
     gene            20829..21755
                     /gene="yqhG"
                     /locus_tag="ACHKTW_002018"
     CDS             20829..21755
                     /gene="yqhG"
                     /locus_tag="ACHKTW_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HFO6463046.1"
                     /translation="MKIILLFLAALASFTVHAQPPSLTVEQTVRHIYQNYKSDATAPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     ADHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPEAFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            21806..22063
                     /gene="yqhH"
                     /locus_tag="ACHKTW_002019"
     CDS             21806..22063
                     /gene="yqhH"
                     /locus_tag="ACHKTW_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HFO6463047.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(22106..24325)
                     /gene="ygiQ"
                     /locus_tag="ACHKTW_002020"
     CDS             complement(22106..24325)
                     /gene="ygiQ"
                     /locus_tag="ACHKTW_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HFO6463048.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTPGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VTVQPPRPKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKNPLAKIGYK
                     SEDVFVPKGDKQRRLHKALLRYHDPANWPLIRQALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRPVNAGAKKRPKAAVGR"
     gene            complement(24436..25848)
                     /gene="ftsP"
                     /locus_tag="ACHKTW_002021"
     CDS             complement(24436..25848)
                     /gene="ftsP"
                     /locus_tag="ACHKTW_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HFO6463049.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(25923..26660)
                     /gene="plsC"
                     /locus_tag="ACHKTW_002022"
                     /gene_synonym="parF"
     CDS             complement(25923..26660)
                     /gene="plsC"
                     /locus_tag="ACHKTW_002022"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HFO6463050.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(26893..29151)
                     /gene="parC"
                     /locus_tag="ACHKTW_002023"
     CDS             complement(26893..29151)
                     /gene="parC"
                     /locus_tag="ACHKTW_002023"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFO6463051.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            complement(29697..30179)
                     /gene="ygiV"
                     /locus_tag="ACHKTW_002024"
     CDS             complement(29697..30179)
                     /gene="ygiV"
                     /locus_tag="ACHKTW_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFO6463052.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDDPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGVTEQRLETDVYVPLA
                     "
     gene            complement(30232..30624)
                     /gene="ygiW"
                     /locus_tag="ACHKTW_002025"
     CDS             complement(30232..30624)
                     /gene="ygiW"
                     /locus_tag="ACHKTW_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HFO6463053.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSATQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            30776..31435
                     /gene="qseB"
                     /locus_tag="ACHKTW_002026"
     CDS             30776..31435
                     /gene="qseB"
                     /locus_tag="ACHKTW_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing response regulator transcription
                     factor QseB"
                     /protein_id="HFO6463054.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLILTARDALEERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            31432..32781
                     /gene="qseC"
                     /locus_tag="ACHKTW_002027"
     CDS             31432..32781
                     /gene="qseC"
                     /locus_tag="ACHKTW_002027"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_306641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing histidine kinase QseC"
                     /protein_id="HFO6463055.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGDKDQWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHYGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPREDLLQSSVMDIYHTAQQAKID
                     VRLTLNAHGIKRTGQPLLLSLLVRNLLDNAVRYSPQGSVIDVTLNADNFIVRDNGPGV
                     TPEALARIGERFYRPPGQTATGSGLGLSIVQRIAKLHGMNVEFGNAEQGGFEAKVSW"
     gene            complement(32827..33135)
                     /gene="ygiZ"
                     /locus_tag="ACHKTW_002028"
     CDS             complement(32827..33135)
                     /gene="ygiZ"
                     /locus_tag="ACHKTW_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2645 family protein"
                     /protein_id="HFO6463056.1"
                     /translation="MFEALLYIMLTYWLIDAFFAFNKYDWMLESGDNICSIPSVSGED
                     RILQAMIAAFFLLTPLIILILRKLFMREMFEFWVYVFSLGICLVCGWWLFWGRFIFCY
                     "
BASE COUNT         8131 a         8873 c         8378 g         7755 t
ORIGIN      
        1 atattgaata ttttgattaa tccccgtact gattattctt cataatcagt acggggttgc
       61 tacaacatgt attacttttt caccacaatt aacggttcaa tatcactctc ttttttgatg
      121 accagtgatt catcaccgcg caaacacgtc ccaccgtagt tgccgcccac ggtgaaggta
      181 catacctgaa tgtatttacc gtccactttc ggcaaacacc acagttgctg atagatgttt
      241 ttctgctcgg caaatttacc gctggttttg tccagcacct cttcatgatg gctgaccagg
      301 tcgatattgc taccacagcg accggcaatc ggtttcactg cgtaacctgt tttcaccaat
      361 tcatcattaa cagtgaaatc ggtatccagc aggtaacgat ggtgcgggaa cagcgaccag
      421 aggatcggca gaatcgcttt gttgccgggg atcaccgtcc acagcggctc aaagactagc
      481 acttccgggc gcagcaatac gtcgataaga cgcacttcgt tttgcggatg accggtacgg
      541 attggcaccg cagcaaactc acggtcgcta acttcacgaa tctgatcaaa cgcggtttcc
      601 cacgcccagg ttttccacac gcagttaacc agtcgccctt ccccatcaat cagttgcccg
      661 gcagcatccc agcccagttc atccaatcca cgcaagatac gcgtttcaaa gcccgcctgg
      721 tgcagcgcct gctccataaa ctgcgcgtga tagttttcct cgatatcttt gtcctgcatg
      781 atatggacaa acggacgtgc acgactgtgt ttccaggcac cagccaattc gttaatcagc
      841 ccttccgccg gattgaagcc gttgccttta tagccctgct ccgcccaacg ttcgaggatc
      901 aagcccgctt cggtatgaca ggaggcggag tcggcgttgt actcgtaaac cttcaggcca
      961 cgctcatcca tgcagaaatc catacgacca gtgatcatat ggtgacggcg acgctgccag
     1021 gagagacgca aacgtggcca gaggattttc gggatgtcga acagcgccag caggttgtca
     1081 tctttcagca ccttgtcggt tgcgtgaaga tacatcaggt gcagctcgtt ggtggcttta
     1141 attagctcct gctcggcact ctcggtaatg gtgtagtaat gataaggatc ctgattgatc
     1201 acctgaccgt tggcctgcac ataggcgttt tgcagcggat ctttttcatc cagccattta
     1261 ccgtcaaact ggcctttgtt ttccaggcgc gctccgctga ttttcagcag ctcgcctgca
     1321 atttccggct gcggtaagct gtattcagta tcttccgtct ggatcatcca gcccagaatg
     1381 gtggtgtcat caaaagtgtc tttcagggta tagcagccgt tttcgaccac catctccagc
     1441 tcacgcgtcc actgttgccc ttgcggcaac ggggaatgaa tcacgttctg ttccgcaata
     1501 cggactttgt tgccatgcaa ttgggtaatg atggcgacat ggccagtgtc tttaaattca
     1561 ccgcctttat cccagataag aagcgcaccc gcgaccggcg cacgcggcga gccgttagga
     1621 aatgcctgca atggcaggat gttgtcatta accacttctc gcaggaagcg cagcgagaaa
     1681 atctcccacg ccatacccac gtcagtaaag accacaccgt aattcagaaa gagaaaacgg
     1741 cgagcaaatt caacgcattg ccacttgtgg cccatatatt cgtcgtcgat atagctacgg
     1801 aatacggcgt catcttcgta ttcctgcgga tcgagagaac tgtaatctga agagtagatt
     1861 gctaccccac ctggggcgta gcccaataat gtcccgaacg gggcatcctg gctggtcgtt
     1921 cctttgctca tcactttacc tttaacaata caacctaagc agttggcgta attgttgtgc
     1981 tctgattacc tgctcagcga cattaaccgg acagaggtca gactaatcat acacctcatc
     2041 gcagcggctg gcgcaaaggg ttaaaaaatt cacattctgt acagcaagtg acctgctaca
     2101 ctgcttcaac actaccactc agaaggcaac tcactatgac agacaatact tatcagcccg
     2161 cgaaagtctg gacgtgggat aaatccgctg gcggcgcgtt cgccaatatc aatcgcccgg
     2221 tttctggtcc gacgcatgaa aaaacgctgc ccgttggcaa acacccattg caactttatt
     2281 cgctgggaac gccgaacggt cagaaagtaa cgattatgct tgaggagctg ctggcgctgg
     2341 gcgttactgg tgcagagtac gacgcctggc tgattcgtat tggcgatggc gatcaattct
     2401 ccagcggctt tgtcgaagtg aacccaaact cgaagatccc ggcgctgcgc gatcatacgc
     2461 ataatccgcc gatccgcgtg tttgaatctg gttcgatcct gctttatctg gctgagaaat
     2521 ttggctactt cctgccgcag gatttggcaa agcgtactga aacgatgaac tggctgttct
     2581 ggttacaggg cgcggcaccg ttcctcggcg gtggttttgg tcacttttac cattacgcgc
     2641 cgatgaaaat tgagtacgcc atcaaccgct ttaccatgga agccaaacgt ctgctcgacg
     2701 tgctggataa gcaactggcg cagcataagt ttattgcggg cgatgagtac accattgcgg
     2761 atatggcgat ttggccgtgg tttggcaatg tggtgttagg cggcgtgtat gatgccgcag
     2821 agtttcttga tgcgggcagt tataagcatg tacaacgctg ggcgaaagaa gtaggcgaac
     2881 gtccggcggt gaaacgtggg cgtattgtta accgcaccaa cggaccgctg aatgagcagt
     2941 tgcatgagcg ccatgacgcc agtgatttcg agacgaatac ggaagataag cgtcaggggt
     3001 aagggttggt gttcgtcgca gcaagccatc caggccggat aaggcgttca cgccgcatcc
     3061 ggcaatcgtg cacaatgcct gatgcgacgc tgccgcgtct tatcaggcct acgaaagcta
     3121 atcatcacgc gctggtaatg tcgtaatcca tttggcgcag tgcgtctaat gtggctttgg
     3181 cttcatcttc gtcgatgatg ctcatggcaa atccgacgtg taccagcacc cactggccca
     3241 gcagatcggc agggttacct tcacaaatca gggcgatatt cacgtcgcgc ttgataccac
     3301 atacttcaac ctgcgcaagc tggtgaatat cttcaccgac agccagcacc tggcctggaa
     3361 cgccaataca cattattaac tccggttatt caacttcaat acttttgacg atcagcgaat
     3421 cgccggtatc gacacgcaac cgctcgccat gacagatcgg acactgcgca tcgtgctgat
     3481 gaatctccac cacctggctg caatcccagc accaggcctg ggcgggttta tagacgatat
     3541 gtaaatcgca cccttgcgcc accgttccgt ggcagacaat ttcaaaacta aaacggacgg
     3601 cgctctcctc aacgcaggag agcgcgccaa tttccagcca cacggcggtg acgcgcttaa
     3661 catcgtgctg ctccgcctgc cgttggataa tttcaacggc gctctggcaa agcgacaact
     3721 catgcatttt cgccactcct gcgaccaaac agcagggcgc gacgccctgc gtgtggtaca
     3781 tccggattcg tgactggcag cgaaaggatc attcgtgcgc agtcatccgt caggcgttgg
     3841 ccctcttcaa tcgacatact gtgcgaaagc ggtgacatca gcgaacagga gaggtattgc
     3901 gaaacaccgt ccagttcgcc aacggtaaag gtcatggtgc catacggcag ttgcagacca
     3961 attttttcac tcactttgcg cagcggccag agttgatccg ggccggggaa gataactgca
     4021 ctcagcatcc acggggtgat cacacacccc gtccactgac cttcgaacag cgtaaaatca
     4081 gaaacataca ccggcattga aggatgcaga aaagagagat cgtgcatcga acgccgggca
     4141 atttcttcaa acgctgcctg tacttgcgcc ttcggggagg tctggaaacc tgctatctct
     4201 tcagtcatga atcgcctccc gtgggatggc ttccacgcct gattcgcgca gcgcagccag
     4261 aacctgctca agcgcaggtt caatcattgc ttcaaccgtc ggcgttaagc cgatgtgtgg
     4321 ctccagcgat tccgggatca cgccgaccag ggtcagcttt ttcggaaact cgccggtgaa
     4381 gcgcagggcc gacaagacgt cggccaggcc aagctgatgc ggagagattt tgttggtaaa
     4441 caacgccgga acttcttcat cccgcaggat catcatcgtt cccggcgcgc tcttcttcga
     4501 cacaatggca tccgcaataa tcagatgatc gcgatttgcc atgtcgccaa gcagctccat
     4561 tcccgccgtg ccgccatcga ggatctcaac ataatccggc agaatgtatc gttgctctaa
     4621 cgcttcaaca atccgcacac cgatggcttc atcggtcagc aaaatattgc cgacccctaa
     4681 gactaaaata cgcattacag aaccttcact gaaaccactt cgttgccgtc ggcatccact
     4741 acgtgtaccg cacaggccat gcacgggtcg aaggagtgaa tggtacgcac cacttccagc
     4801 ggtttattcg gatcagcaac cggtgtaccc accagcgact gctcgtaagg accgacgtca
     4861 tcattgaagt tacgcggacc agagttccag gttgatggaa caactgcctg gtagttgctg
     4921 atgataccgt ctttgatcac catccagtga gagagcatac cgcgcggcgc ttcgaggaag
     4981 ccaacacctt tgaactcacc cgttgccgga atgttcggtt tcacgaaggt ggtgtgatcg
     5041 cctttgccga tattggtgat cagtgcactg tattggtttt gcaggatatc ctgcaattcg
     5101 cagcagtgaa cggtacgacc aataatacgg cccagcgtag agtgcagttg cgccacttcc
     5161 agcgtgttac cagtcagttt ctgataaatc gcaacgattt cattcagttt gttctgggta
     5221 gattctcgac ctgccgccag tttcaccagc atattagcca gcggacccac ttctaccgtt
     5281 ttgccgtaga aagtcggtga tttcacccag gaatatttcc cgtcgtcaga ccaaccatca
     5341 taagccggaa tggtggtacc ttcccacggc gcctgtggcg cttcgtcttt ataccaggag
     5401 tgcttcgcgc tttcctgaat gcctttgatc aggtattcat cggaatgaga agtgatcgga
     5461 cgatacgagg acagatccgc attctcaatg tagccgcccg ggaacaggaa gctgccgttt
     5521 ttgctgtcgg tcgggaattc cggcacgctc aggtagttca ccgcaccttt accgcgcgtc
     5581 agccattccg ggtagaacgc ggcgataacc gcggtatcaa ccttataaac ctgctcaaca
     5641 aagtcgctca gtttgtcgat gaaagacttg atgtacatca ggcgctcaag gttcaacacg
     5701 cccaacccgt cgaggttgat tgggttcgcg acaccaccca ctgccaggtt ctgaatgtgc
     5761 ggcgttttac cgcccagcag cgccacgacg cggttagcgt cacgctggca ctccagcgcc
     5821 tgcaggtagt gcgctaccgc aatcaggttc acttccggcg gcagtttcat cgctgggtga
     5881 ccccagtagc cattagcgaa aatacccaac tgaccgctgg caaccagatc tttgatcttg
     5941 ttctgaactt tggtgaactc ttccgggctg ttcagatgcc aggtcgaaac gcctttcagc
     6001 atttcggagg ctttggttgg gtcagcttgc agtgcagaag tgatatccac ccagtccagc
     6061 gccgaaagct gatagaaatg aacgatatgg tcatgcgtgg tgtgcgcagc cagaatgatg
     6121 ttacggatgt attgcgcgtt aaccggaacg tcgatattca gcgcactttc tgccgcacga
     6181 acggaagaca gcgcgtgagt ggtagtacat acgccacaga tacgttgcac aatcatccat
     6241 gcatcgcgcg gatcgcggtt tttcacgatc tcttccatgc cgcgccacat ggtaccggaa
     6301 gcccatgctt tcgaaacgac gccattttcg atttcgcaat cgatgcgtaa atgcccctca
     6361 atacgggtta ccggatcaat agtaattctc tggctcatgc tttgctcgcc tcatgacgat
     6421 tatgatcgtt ttgttttaaa ggaggaagta tcggcagtag acgaatgagt acgatgtaag
     6481 cgcaaatctc aatagccaca aaaccaatag aaatcaacag ttcttcccag gtcgggaagt
     6541 aggcgtaacc gccgcccggg ttgaatgcca ccagcgaata ggtcagacgc caggttgcac
     6601 aacctaacag tgcgctcagt gctgacagga acagcatgcg ggaatcatta cgcagcttcg
     6661 ccacacgcag aacgaccagc gggaagagca tcagcaggac ttcaatccag aacatcacgg
     6721 agtagaagtc accggcaaac gctaacgaca gcttgtcgcg atagataagc tcgccaaagc
     6781 gcagcacgat gaaaatcgcc agcaacacac tgatggtgtt ggtcagcttg acgaacaggc
     6841 tcttttcatc cggaccgttg ccacgcagac ccgcctgcac cagtgaacct tcaaagatga
     6901 caatcgagaa gcccatgatg aacgccgtca gcagcgagaa cagcggcaac atttcatagc
     6961 tctgccacag cggatgcacc ttgtagcccg ccgagatcat cagcgacccc attgaagact
     7021 ggtgcatggt cggcagcaac gcaccgagcg cgatgatgaa gaacatcacc ttgttcagac
     7081 gctgtagcga caccttccag cccagacgtt caaacagtgc cggagcaaac tccagagcca
     7141 tcacgccgat gtagatggtc atacagaccg ccgtctcgaa cagtaccgag ttcacgttga
     7201 agtgacccgg aatgtagaag tacggcaggt tccagtagcg acccacgtcg atagtgatcg
     7261 acaagccacc cagtgagtaa ccaaacagac tcgccaacag cgccggacgc accagcggat
     7321 ggtattgccc acggttaaag acgtataccg cccacgccag cgcccagccg ccacaggcaa
     7381 agccagtgcc gatcagcagg tcaaacgcga tccacacgcc ccacggaaag ccgccgttca
     7441 ggtcggagac agagcccaga ccgaacacca gacgcttcac aatcaggagc atacagatga
     7501 cgattaacgg tccaaaaatc atgaccggtt tactgatgat tttgccgccc agcggttttg
     7561 gatcatgact catgatcgtc tcctccgtcg tgatggtcgt ttttggtgtt gcgacgaacc
     7621 agcacggtta agcccgccag cacagccagt ggtagcatca tgcctttata cagggtgtgt
     7681 tgaatatttt cggaacgcgc accggtagaa agatcgtcca gtttcggcag gtcgagattt
     7741 tcataaggca cacccgtcag taccagaacc tgagtaccgc cgccctcttt ctcgccgtac
     7801 agatgcggat aatatttcgg caccgtatgc aggtaagtgt cgccagattt cagcgtctga
     7861 cgcggatagt ggtattcgct gccaggcttc agcgccagac gttttttcgc ctccgccatc
     7921 agctcttcac gcgtaccgaa aatcaccgcg cccgccgggc acacttctac gcagccaggc
     7981 agaccgcctt tatcgagacg ttccacacct ttctggttgc acagctcgca cttatgcagc
     8041 gcaccaaacg ggttgttgta gtcgtacttc ggcacgttgt acggacaggc gaccatgcag
     8101 taacggcaac cggtgcacac gtctttgtcg taatggacaa tgccggtttt cggatctttt
     8161 ttcagtgcag agaccgggca cacagagaca cagttcggat cgacgcagtg catacactgt
     8221 ttcttaatgt acgcgtagcc gttctcctcc tggtctttgt tgacccctgt gccgctggtc
     8281 cacacctgaa tgatgttatt ggtatacggc gacagtttgt cgttgttcga ccaggtctgt
     8341 tccccttgcg ggttacgttc agggaaattg atatcctgac acttggtgac gcaagcctgg
     8401 cagcctacgc acaaggtcga gtcgtacaac atccccagcg atcccggaat tggcgggcgg
     8461 ttttcagcag ccgcatgact gacagacggc agcgcgcccg tcagcaatgc cccgcaggag
     8521 gctgctttaa taaaattacg tctgttcacg gttattctcc ccgtgagtca gcgttatctt
     8581 tcttttgctg acgacccagt tcacgcaccg ccatcacgct gacaccggca accaacccaa
     8641 ccacaccgcc gagcaaacca atagcgcctg cagagacgtt gccgccctct ttagcgttaa
     8701 catccggttt ctgtgaacgc ggagtttgat tttcgacgtt ggcaagctga tggatgcctt
     8761 tatggaagcc gataccttct tcgttacagc catagcacgg gtgaccaatc gccaccggcc
     8821 acacaccgcc aacatcgcag aattgcagcg ttgagcagtt gccgtaagtt tctggccctt
     8881 tacagccgag gtggtacagg caccagcctt cgcggtggcc ttcatcaccg aactctttgg
     8941 caaaacgacc agcatcgaag tgcgggcgac gttcgcagtg ttcgtgaatc agacggccat
     9001 aggcgaaggt cggacggttt ttgtcatcca gtttcggcgg tttgccgtaa gtgatgatgt
     9061 gcgcaacggt cgcgaggaag ttgtgcgggt tcggcgggca gcccggaata ttgataacgg
     9121 ttttgcctgg cagaacttct tgcaggctga ctgcgccagt tgggttaact ccagctgcgg
     9181 caacaccgcc ccacgcagag caggaaccga tagcgataat ggctgctgcg ccttccgccg
     9241 ctttgcggat gtgatccaca ataggctcac cggcaaccat gcaataaata ccgttatctt
     9301 ttaatgggat ggaaccatcc accactaaca catactgccc tttgtacttc tcgagagcgt
     9361 tatgtttgtt ctcttcgacc tgatgaccga aggcggcgga aagcacttcg tgatactcca
     9421 gagagatagt ctccagtacg aggttttcta ccgttggatg cgttgcacga agcagagatt
     9481 ccgtacaacc ggtgcactcc tgcgcgccaa tccagataac tggcggacgc tgcgggttag
     9541 taaccgattc ggccatctct gcagcggctt tgctacttaa ccccatggtg gcggctaatg
     9601 ctgcacaaag cttcatgaaa tcacgacggt taatgccgtg agaatggatg agggtgttat
     9661 ctccagtcat ttatagttat tccgttgcga agacctggca tatattttgc ctcaatcgca
     9721 aaatcaataa tgcgatcgat gcgccattta ccacacattt attatggtta tcgatatcat
     9781 gatactgcgg cggggcgcta aaacgaaggg aagaagcatt agtgtaatta attaaagcaa
     9841 gataatacgt atgtttgatc aattttcgag gcgataaccg gccataaaaa agcgcaacgt
     9901 agaacaggaa ttattctcac gaaagaaatc cattaccatc tctttatcca gtccatagcg
     9961 gcgcgtcaga atacccgctt cccaggcgct aagctggcga tctccggtct tttcgtacat
    10021 ccggtgtgag tagcctaata caaaaccgcg tttataatcg gcacagaatt ttgtcacatt
    10081 aaccgcactg tcggcatgcg ttgcttttaa tcccgccatt aagcctttac caaaatggtt
    10141 attcatcgtt cacctcattc gctatatatt gtcatatata gcgatatttt aggcaaagaa
    10201 cgacttttgc gcacgctatt ttttcatgct tgtttaagac gtaatcccgt aagagataaa
    10261 cctatcagga gagatattta tcgaaccatt tcagtgtcct ttgccaggca agatcggcgg
    10321 cagatttgtc ataacggggc gtggaatcat tatggaatcc gtgattaacc cccggataga
    10381 tatacgcctc ataaacctta ttattggctt tcaacgccgc ctcgtaagca ggccagccct
    10441 cgttgattcg ggtgtccagt tccgcgaagt ggagtagtaa aggcgcttca atcttcgcca
    10501 catcggcagt gggtgcctga cgaccataaa acggcaccgc gcaggccagt tccggatacg
    10561 cgacagccgc cgcgttcgat acaccaccgc catagcaaaa tccggtaata cccactttgc
    10621 ctgccgcttg cggatagcgt tgcataaact caattgcggc aaagaaatca ttcatcagtt
    10681 tggttggatc aacctgctgt tgcagctcac gacctttatc atcattaccc ggataacctc
    10741 caacggaact taagccgtca ggtgccaggg cgatataccc cgccttcgcc actcgccgtg
    10801 ccacatcttc gatatacgga ttcagtccac gattctcatg caccaccacc acggctggcg
    10861 ttttgccgct catccttgcg ggtttcacca gataaccccg tacctcgccg tgaccatttg
    10921 gcgaaggata cgtgatgtac tcagcaacaa tctccgggtc ggtaaactct acctgagtcg
    10981 ccagcgcata atttggcttg agcaaatcaa acaacgctaa tgccgtcatc ccgcctaccg
    11041 catacttcgc cgcaaggttg aggaactcac gtttcgagat tttcccgtga gcgtaatagt
    11101 cgtagtaatc caacaactct tgtgggaaat ctttggcggt taaacgcggc atcgttgcac
    11161 tcctcaactg gtgttttttt aagcaaagca taagcacgtg ttttcgccca gtttttcgtc
    11221 actctgtgag ccagactacg ggatacgcgc tggcgaatcg ctaaactaga aacattgttt
    11281 cgaaattgaa cggtggaaag gagaggtcat ggtctggtta gcgaatcccg aacgttacgg
    11341 gcagatgcag taccgctatt gcggaaaaag cggtttacgc ctgcccgcgt tatcgctcgg
    11401 tttatggcac aatttcggtc acgttaacgc gctggaatca cagcgtgcga tcctgcgtaa
    11461 agcgtttgat ttgggcatta cgcactttga tttagccaac aattacgggc cgcctccagg
    11521 aagcgcagaa gagaactttg gtcgcctgct gcgggaggat tttgccgctt atcgcgatga
    11581 actgattatc tctaccaagg ctggctacga tatgtggccc ggcccgtacg gctctggcgg
    11641 ttcacgtaaa tacctgctcg ccagcctcga ccaaagcctg aagcgtatgg ggcttgagta
    11701 tgtcgatatc ttttactctc atcgcgtcga tgaaaatacg ccgatggaag aaaccgcctc
    11761 tgcgctggct catgcggtac aaagcggtaa ggcgctgtat gtcgggatct cctcttactc
    11821 gccagagcgg acgcaaaaaa tggtcgagtt gctgcacgag tggaaaattc cgcttttaat
    11881 tcatcaacct tcgtacaatt tactgaaccg ctgggtggat aaaagcggcc tgctggatac
    11941 cctgcaaaat aacggcgtgg gctgtattgc ctttactcct ctggctcagg gattgctgac
    12001 cggaaaatat ctcaacggca ttccgcaaga ttcacggatg catcgtgaag ggaataaagt
    12061 tcgtggtctg acgccgaaaa tgcttaccga agccaacctc aacaacctgc gcttattgaa
    12121 tgaaatggca cagcagcgtg gacaatcaat ggcgcaaatg gcgttaagct ggttgctgaa
    12181 agatgatcgc gtgacgtcgg tattgattgg tgccagccgc gcggagcaac tagaggagaa
    12241 cgtgcaggcg ctgaataatc tgacatttag caccgaggag ctggcgcaga ttgatcagca
    12301 tatcgccgat ggcgagctga atctgtggca ggcgtcgtcc gataaatgac ctgttaataa
    12361 cgggcgcgcg aaccgcgccc gaatcagatc agtgattatg acgagtcagt cggtcaagat
    12421 agcccatcac aaatgcagaa agcacaaacg tcagatggat aatgacgtac cacatcagtt
    12481 tgttatccgg gacatttttc gcatccataa agacgcgcag taggtgaatg gaagaaattg
    12541 ccacaatcga cgctgctact ttgtttttca gcgacgttgc gtccattttc cccagccagt
    12601 tcagcttctc tttgttctcg gagatatcca gctgtgagac gaaattctca taaccggaaa
    12661 acatcaccat caccagcaaa ccgccaacca gcgtcatatc caccagcgac agcaacacga
    12721 ggatcaaatc tgattccgcc atcgagaaga tattcggcag tacgtgaata atctcctgga
    12781 agaacttcag cgccagggca actaacgcca gcgaaaggcc aaagtacacg ggggcaagca
    12841 gccagcgaga agcatacatt gcattttcaa gaaaacgttc catacattcc tgtcgtgtag
    12901 ttaaaacggg caacagtata tcgcaattga gtaaacagca cgcaacagca aatcaacata
    12961 tacgttaaac ctttgtcgca taattctgag gtttagggat cttcctggtt cccttatcaa
    13021 cgcatcgaga acgtctaccc ttaaaggacg ttgaacctga agggagaaaa acgatgtctc
    13081 atttaaaaga cccgaccacg cagtattaca ctggtgaata tcccaaacag aaacaaccga
    13141 cgccaggcat ccaggcgaag atgacaccgg taccggattg cggcgagaaa acctatgttg
    13201 gtagcggtcg cctgaaagat cgtaaagcac tggtgacagg gggcgattcc ggaataggtc
    13261 gcgctgccgc catcgcttac gcgcgtgaag gggctgacgt ggcgatcagt tatcttcccg
    13321 tggaagaaga agacgctcag gatgtgaaaa agatcattga agaatgcgga cgcaaagccg
    13381 ttctgctgcc aggcgattta agcgatgaga aattcgcccg ttcgctggtt cacgaagcgc
    13441 ataaggcgtt aggcgggctg gatattatgg cgctggtcgc cgggaaacag gttgccattc
    13501 ctgatattgc agacctcacc agcgaacagt ttcaaaagac ctttgccatt aacgttttcg
    13561 cgctattctg gctaacccag gaagcgatcc ccctgctacc gaaaggtgca agtatcatca
    13621 ccacttcgtc aatccaggca taccagccaa gtccgcattt actggactat gcggctacga
    13681 aggcggcgat tctgaactac agccgtggcc tggcaaaaca ggtcgcggag aaaggtattc
    13741 gggtgaatat tgtcgcgcca ggcccgatct ggacagcact gcaaatttcc ggcggacaaa
    13801 cgcaggataa gatcccgcag tttggtcagc aaacgccgat gaagcgtgcg gggcaaccgg
    13861 cggaactggc ccctgtatat gtttatctgg caagtcagga gtcgagctac gtcaccgcag
    13921 aagtgcacgg tgtgtgcggc ggcgagcatt taggttaaaa aaatgcccgg ttgtgaaaag
    13981 caaccgggca tcatttttat ttcgctttgg cggtttcttc gccgaccaga cctatcttca
    14041 ggtaacccgc ctgatgcagc gtatccatta ccttcatcaa cgtctcgtaa tcgacggttt
    14101 tatccgctcg gaagaagatg gtggtgtctt tcttgccttc ggttaacgca ttcaacgccg
    14161 taatcattgt ttcatcggtg accggatcgt taccgataaa catcgagttg tctgccttca
    14221 ccgacagata aaccggtttt tccgggcgcg gctgcggcgt gctggtagaa gcaggcaaat
    14281 tcaccttcac atctaccgtc gctaacggtg ccgccaccat aaagataatc agcagaacca
    14341 acatcacgtc gataaacggc gtcacgttga tatcatgcat ttcgccgtta tcatcgaggt
    14401 tttcgttaag atgcattgcc atcggacatt atcctgcgcg taatttttgt gcgacacgaa
    14461 ccggatgcgc agcggcgctg gcttccagat ccaggtcacg gctttgcagc aacaatacct
    14521 gcgctgcaac atcacccagc atcgctttaa agccgccaat ctggcgtgca aatacgttat
    14581 agataacgac cgcaggaatc gctgccacga ggccgattgc cgttgctaac agagcttctg
    14641 cgatacccgg cgcaacgact gccaggttag tggtctgcgt ttgcgcgata ccaataaagc
    14701 tgttcatgat gccccagacc gtaccaaaca gaccaacgaa cggagaaatc gcgccgatgg
    14761 ttgccagata gccgttaccg cggcccattt gacgacccac tgcggcgacc cgacgctcca
    14821 gacggaagct ggtacgttct ttaatacctt cgttatcgtc gctgccttct gacagttcca
    14881 gctcattctg cgcttcattg agcaaatgca ggcttaagct tttgctacca aaatcagcgg
    14941 cgatatcgtt ggcctggttt aaggaacgcg cttcagccag cagttgctgc tcgcgcttaa
    15001 ggcgacgctt ctgattgaag aactctacgc tcttactgaa gaagattgcc caggtgacta
    15061 cggaggccaa aataagccca atcatcacgc acttaacgac aatatcggcg tgctgataca
    15121 taccccagac ggaaaggtcc gtctgcatta aattattacc cactgtgtat ctccaggacg
    15181 caagtcacaa aatctgcgca taataatatc aaaacgacgt cgaattgata gtcattctca
    15241 ttactatttg catactgccg tacctttgct ttcttttcct tgcgtttacg cagtaaaaaa
    15301 gtcaccagca cgccatttgc gaaaattttc tgctttatgc caattcttca ggatgcgccc
    15361 gcgaatattc atgctagttt agacatccag acgtataaaa acaggaatcc cgacatggcg
    15421 gacaaaaagc ttgatactca actggtgaat gcaggacgca gcaaaaaata cactctcggc
    15481 gcggtaaata gcgtgattca gcgcgcttct tcgctggtct ttgacagtgt ggaagccaaa
    15541 aagcacgcga cgcgcaatcg cgccaatggc gagttgttct atggacggcg cggaacgtta
    15601 acccatttct ccttacaaca agcgatgtgt gaactggaag gtggcgcagg ctgcgtgcta
    15661 tttccctgcg gggcggcagc ggttgctaat tccattcttg cttttgtcga acagggcgat
    15721 catgtgttga tgaccaacac cgcctatgaa ccgagtcagg atttctgtag caaaatcctc
    15781 agcaaactgg gcgtaacgac atcgtggttt gatccgctga ttggtgccga tatcgttaag
    15841 catctgcagc caaacactaa aatcgtgttt ctggaatcgc caggctccat caccatggaa
    15901 gtccacgacg ttccggcgat tgttgccgcc gtgcgcagtg tggtcccgga tgccatcatt
    15961 atgatcgaca acacctgggc agccggtgtg ctgtttaagg cgctggattt tggcatcgat
    16021 gtttccattc aggccgctac caaatatctg gttgggcatt cagatgcgat gattggcact
    16081 gccgtgtgca atgcccgttg ctgggagcag ctgcgggaga atgcctatct gatgggccag
    16141 atggtcgatg ccgataccgc ctatataacc agccgtggcc tgcgcacatt aggtgtgcgt
    16201 ttgcgtcaac atcatgaaag cagtctgaaa gtggctgaat ggctggcaga acatccgcaa
    16261 gttgcgcgag ttaaccaccc tgctctgcct ggcagtaaag gccacgaatt ctggaaacga
    16321 gactttacag gcagcagcgg gctattttcc tttgtgctta agaaaaaact cagtaatgaa
    16381 gagctggcga actatctgga taacttcagt ttattcagca tggcctactc gtggggcggg
    16441 tatgaatcgt tgatcctggc gaatcaacca gaacatatcg ccgccattcg cccacaaggc
    16501 gagatcgatt ttagcgggac cttgattcgc ctgcatattg gtctggaaga tgtcgacgat
    16561 ctgattgccg atctggacgc cggttttgcg cgaattgtat aacattgcca cttttggaca
    16621 attttgcaga catttaattg tgaaaagtct taaattgttg cgtccgggat caaggcgtcc
    16681 cggacgaatc aggagtacaa taggcaaata aaggcttaaa cgctgttcca caggaaagtc
    16741 catggctgtt attcaagata tcatcgctgc gctctggcaa cacgactttg ccgcgctggc
    16801 ggatcctcat attgttagcg ttgtttactt tgtcatgttt gccacgctgt ttttagaaaa
    16861 cggcctgctg cccgcctcat ttttgccagg cgacagcttg ttgatactgg caggcgcatt
    16921 gattgctcag ggggttatgg attttctgcc tacgattgcg attctgaccg ccgcagcaag
    16981 tctgggctgc tggctaagtt atattcaggg gcgctggtta gggaatacca aaacggtgaa
    17041 aggctggctg gcacagcttc ctgctaaata tcaccagcgc gccacctgca tgtttgaccg
    17101 ccacggtctg ctggcgctgc tggctggacg ttttcttgca tttgtccgta cgctgctgcc
    17161 aaccatggcg ggaatttccg gtctgccaaa ccgccgcttc cagtttttca actggttaag
    17221 tggattgctg tgggtcagcg tggtaaccag ttttggctat gccttaagta tgattccgtt
    17281 cgttaaacgc catgaagatc aggtaatgac gttcctgatg atcctgccaa ttgccttgtt
    17341 aaccgctggc ttgttaggca cgctgtttgt ggtgattaaa aaaaaatact gtaacgcctg
    17401 acgattttcc ccgttcccgg ctgctgtacc gggaacgtat ttaattcccc tgcatcgccc
    17461 gcattcttgc cgcatcttcc cccggcgtca caccgaagta acgtttaaac tcacggctaa
    17521 attgcgatgc gctttcatag ccgacgcgca tcgctgctgc gctagccttc atgccgtcat
    17581 ggatgatcat catccgcgcc ttatgcagac ggtaattctt caaatactgc aacggcgagg
    17641 tgctggtgac agacttaaaa ttatggtgga acgccgatac gctcatgttg gcttctgccg
    17701 ccagttgctc gacgctcagg ttttcggtgt atttattctc aatccgtttc agcacgcggc
    17761 taatcagact gaagtgagtc tggcgactga ccagcgccag taacgcgccg ccgcaaggtc
    17821 cggtcagcac gtagtacaga atttcgcgga tgatctgttt gccgagaata cgcgcatcca
    17881 gtggtcgctc catcacgtcg agtaaccgct ccgccgcgca taaaatctct tctgataacg
    17941 tggcggagtt aatcccgctg gctgccatcg acggctggaa atgctcatct tcgccaatgt
    18001 ccatcaacag ttcctgtaac tgcaaaatat cgacattgag acgcaaccct gccagcggca
    18061 cctctgacgt ggcataggtt tcgcactcaa acggcaacgg caccgtcagc agcaggtatt
    18121 cattggcatc ataacgaaac acgcgttcat tgatataacc gattttatgc ccggaaaaga
    18181 gaattatgat gccaggctcg tacatcaccg gtgtacgtgc gaaaggcgtc tcgccataca
    18241 acaaacgcac atcgggcaac agttctgaca aactattttc tttatttttc agtttattaa
    18301 ctttatccgc cagcaagcgg caaatctctt cacgtttcat atcgcgtaat ttcttaggaa
    18361 taatgcggca atttgattgt gcgcaatttt gtagcatttc tccagcactc tggagaaata
    18421 ggcaagacat tggcagaaat gagcattgag agccagggcg ctggcgatca caatgaaaaa
    18481 catcaggcag atcgttctct gccctcatat tggcccagca aagggagcaa gtaatgaaca
    18541 actttaatct gcacacccca acccgcattc tgtttggtaa aggcgcaatc gctggtttac
    18601 gcgaacaaat tcctcacgat gctcgcgtat tgattaccta cggcggcggc agcgtgaaaa
    18661 aaaccggcgt tctcgatcaa gttctggatg ccctgaaagg catggacgtg ctggaatttg
    18721 gcggtattga gccaaacccg gcttatgaaa cgctgatgaa cgccgtgaaa ctggttcgcg
    18781 aacagaaagt gactttcctg ctggcggttg gcggcggttc tgtactggac ggcaccaaat
    18841 ttatcgccgc agcggctaac tatccggaaa atatcgatcc gtggcacatt ctgcaaacgg
    18901 gcggtaaaga gattaaaagc gccatcccga tgggctgtgt gctgacgctg ccagcaaccg
    18961 gttcagaatc caacgcaggc gcggtgatct cccgtaaaac cacaggcgac aagcaggcgt
    19021 tccattctgc ccatgttcag ccggtatttg ccgtgctcga tccggtttat acctacaccc
    19081 tgccgccgcg tcaggtggct aacggcgtag tggacgcctt tgtacacacc gtggaacagt
    19141 atgttaccaa accggttgat gccaaaattc aggaccgttt cgcagaaggc attttgctga
    19201 cgctaatcga agatggtccg aaagccctga aagagccaga aaactacgat gtgcgcgcca
    19261 acgtcatgtg ggcggcgact caggcgctga acggtttgat tggcgctggc gtaccgcagg
    19321 actgggcaac gcatatgctg ggccacgaac tgactgcgat gcacggtctg gatcacgcgc
    19381 aaacactggc tatcgtcctg cctgcactgt ggaatgaaaa acgcgagacc aagcgcgcta
    19441 agctgctgca atatgctgaa cgcgtctgga acatcactga aggttccgac gatgagcgta
    19501 ttgacgccgc gattgccgca acccgcaatt tctttgagca attaggcgtg ccgacccacc
    19561 tctccgacta cggtctggac ggcagctcca tcccggcttt gctgaaaaaa ctggaagagc
    19621 acggcatgac ccaactgggc gaaaatcatg acattacgct ggatgtcagc cgccgtatat
    19681 acgaagccgc ccgctaagct ttttacgcct caaactttcg ttttcgggca tttcgtccag
    19741 acttaagttc acaacacctc accggagcct gctccggtga gttcatataa aggaggaacg
    19801 tatggctaat ccaaccgtta ttaagctaca ggatggcaat gtcatgcccc agttgggact
    19861 gggcgtctgg caagcaagta atgaggaagt aatcaccgcc attcaaaaag cgttagaagt
    19921 gggttatcgc tcgattgata ccgccgcggc ctacaagaac gaagaaggtg tcggcaaagc
    19981 cctgaaaaat gcctcagtca acagagaaga actgttcatc accactaagc tgtggaacga
    20041 cgaccacaag cgcccccgcg aagccctgct cgacagcctg aaaaaactcc agcttgatta
    20101 tatcgacctc tacttaatgc actgacccgt tcccgctatc gaccattatg tcgaagcatg
    20161 gaaaggcatg atcgaattgc aaaaagaggg attaatcaaa agcatcggcg tgtgcaactt
    20221 ccagatccat cacctgcaac gcctgattga tgaaactggc gtgacgcctg tgataaacca
    20281 gatcgaactt catccgctga tgcaacaacg ccagctacac gcctggaacg cgacacacaa
    20341 aatccagacc gaatcctgga gcccattagc gcaaggaggg aaaggcgttt tcgatcagaa
    20401 agtcattcgc gatctggcag ataaatacgg caaaaccccg gcgcagattg ttatccgctg
    20461 gcatctggat agcggcctgg tggtgatccc gaaatcggtc acaccttcac gtattgccga
    20521 aaactttgat gtctgggatt tccgtctcga caaagacgaa ctcggcgaaa ttgcaaaact
    20581 cgatcagggc aagcgtctcg gtcccgatcc tgaccagttc ggcggctaac atgcaaattc
    20641 tcccggtggc ggtaatgttc cgctaccgga cttttcagaa atcatttatt cccctcgcgt
    20701 cccgcccgtt gttactcttc cttgttcagg aatgccaaat ataaggacat catcatgcag
    20761 agccggaagc tcttaaaaga acaactcatc tatatccggg ataaacgcaa cggagaggtg
    20821 aaaaacagat gaaaataata cttctgtttt tagcagccct ggcaagtttt accgtacacg
    20881 cacagccccc ctcactgacc gtagaacaaa cagtccggca tatttatcag aactataaat
    20941 cagatgccac tgccccttat tttggtgaaa ccggagagcg ggcgataact tctgcgcgta
    21001 ttcaacaggc gcttaccctg aacgacaatc ttacgctgcc gggcaatatt ggctggctgg
    21061 attatgatcc ggtttgtgat tgtcaggatt ttggcgatct ggtgctagaa agcgttgcga
    21121 taacccaaac tgacgccgat catgccgatg ccgttgtgcg ctttcgtatc tttaaagatg
    21181 ataaagaaaa gaccacgcag acactgaaaa tggtggcgga aaatggtcgt tgggtcattg
    21241 acgatattgt cagcaatcat ggcagcgtct tacaagcagt taatagcgag aatgaaaaaa
    21301 cgctggccgc tttagcttcg ttgcaaaaag aacagccgga agcctttgtt gccgaactct
    21361 ttgaacatat tgctgattat agctggccgt ggacgtgggt ggtttccgac tcttaccgcc
    21421 aggcggttaa tgccttctat aaaaccacct tcaagacggc caataatccc gatgaagata
    21481 tgcaaataga acggcaattt atttacgaca atccgatctg ttttggcgaa gagtcgctat
    21541 tttcacgcgt tgatgaaatt cgagtcctgg agaaaaccgc cgattccgcc cgcattcatg
    21601 ttcgttttac gctgaccaat ggcaacaacg aagagcaaga actggtttta cagcggcgcg
    21661 aaggcaagtg ggaaatcgct gattttatcc gcccgaacag cggcagccta cttaagcaga
    21721 ttgaggcaaa aaccgccgcc agattaaagc aatgagctga attaaataac aattagccgg
    21781 aacaataaat aaaagggaac actatatgaa aacgattttc accgtgggag ctgttgttct
    21841 ggcaacctgc ttgctcagtg gctgcgtcaa tgagcaaaag gtcaatcagc tggcgagcaa
    21901 tgtgcaaaca ttaaatgcca aaatcgcccg gcttgagcag gatatgaaag cactacgccc
    21961 acaaatctat gctgccaaat ccgaagctaa cagagccaat acgcgtcttg atgctcagga
    22021 ctattttgat tgcctgcgct gcttgcgtat gtacgcagaa tgataaaaaa atccccggca
    22081 gcatgtcagt tgccggggat tttttttaac gtccaaccgc cgctttaggg cgtttcttcg
    22141 caccagcatt caccggacga gattgcgtag acgacgcttt ttttgccgta gcaggcgtct
    22201 gacgctgggt cgccatcggc gtatgtttcg tcaacgccgg acgggtattg cggttctggc
    22261 gacgagcttc acgcatctct tcaatggttg gcgcaggcac taagcaatcg cgacggctgc
    22321 caatcagatg ctttttgccc atcgcttcca gcgcctggcg gattaacggc cagtttgccg
    22381 gatcgtggta acgcaacaac gctttatgca aacgacgctg tttgtcgccc ttcggtacga
    22441 agacgtcttc gctcttataa ccaatcttcg ccagcgggtt tttcccggtg taatacatgg
    22501 tggttgagtt cgccagcggc gatgggtaga agttctgtac ctggtcgaga cggaaacgat
    22561 gctttttcag ccacagcgcc agattcacca tatcttcatc acgcgtaccg gggtgcgcgg
    22621 agatgaaata ggggatcaga tactgctctt tacctgcctg ttttgagtaa gtatcgaaca
    22681 gctctttaaa gcggtcatag ctgcccatgc ccggcttcat catcttcgat aacggccctt
    22741 cttcggtatg ttccggggca atcttcagat aaccgccgac gtgatgggtc gccaactctt
    22801 tgatatagcg cggatcttcc acggcgatgt cataacgtac gcctgaggcg atgaggattt
    22861 ttttaatgcc tttcagatca cgcgcacggc gatagaggtt gatcgtcggt tcgtggttag
    22921 tgtccatgtg cggacaaata tccggataaa cgcacgacaa acggcgacaa gtttgttcag
    22981 cgcgtggcga tttgcagcgc aacatataca tgttggcagt tggcccaccg agatcggaaa
    23041 tcacgcccgt aaaacctgga acggtgtcgc ggatcgcttc gatctcatta atgatcgaat
    23101 cttccgaacg gctctgaata atgcgccctt cgtgctcggt aatagaacag aaagaacagc
    23161 cgccaaagca gccgcgcata atgttgaccg aaaaacggat catttcgtaa gccggaatac
    23221 gggcattgcc ataggccgga tgtggcacgc gcttgtacgg cagcgcaaaa acgctgtcca
    23281 tctcttcggt agaaagcggg atagcaggcg ggttgatcca cacatagcgg tcaccgtgtt
    23341 tttgcatcaa tgcgcgggcg cagcctgggt tggtttcgtg gtgcagaatg cgcgaagcat
    23401 gggcgtacag cactttatcg cccttcactt tctcgaaaga aggcagcaac acgtaggttt
    23461 tttcccacgg tttcgggcgc ggtggctgca cggttacggc tttggcttcc tgctttttcg
    23521 gtgccaccgg tttgttatcc gcgcacggca aatcttcacc atacggatgc gggattgggt
    23581 cgatttttcc aggggtatca agacgggtgg aatccacgcc gctccagcca ggcagcgcct
    23641 ctttcacgat aatcgcggta ttacgcacat cgcggatttc actaatcggc tcgcccatcg
    23701 ccagacggtg cgccacctcc accagcggac gctcaccgtt accaaacatc agcatgtcgg
    23761 ctttcgaatc caccagcacg gaacggcgca cggtatcgga ccagtaatca taatgtgcgg
    23821 tacggcgcag actagcctca ataccgccga ggatcaccgg tacatctttc cacgcctctt
    23881 tacaacgctg ggtataaacc agtgtggcgc gatccgggcg cttacccgcg acgttatccg
    23941 gcgtgtaggc atcgtcatga cgtaaacggc gatcggcggt ataacggttg atcatcgaat
    24001 ccatgttgcc agcagtaaca ccgaaaaaca gattcggttt acccagacgc atgaaatcgt
    24061 ctttgctgct ccagtctggc tgggcgatga tcccgacgcg aaagccctgt gcttccagca
    24121 tacgaccgca aatcgccatc ccgaagcttg ggtgatcgac atacgcgtcg ccagtaacca
    24181 aaatgatgtc gcagctatcc cagccaagtt gatccatctc ttcacgagac atcggcaaaa
    24241 acggtgccgg tccaaaacag gcggcccagt actgcggcca ggagaacagg tcgcgatccg
    24301 gttggatcag ggagatagag ctcatattgc ttccagaaat gataaaaaaa taatcaaagg
    24361 ccggggatta taagccggaa cgaaagagaa atcgaaaggt attccatact cgccctcctc
    24421 gggcgagtat gaagattacg gtaccggatt gaccaaaagt tgccctatcg agccacggtc
    24481 cgccatttcc agcgtctgac tgttgaagta gaacgggaag tgcgcccagg aaggctgacc
    24541 gaaataaaca agcagctcca cttgtccgtc aacccaaacg gtatctttcc agcctctgtc
    24601 ttccgggaac ggcatcgcgc cattcacgtt acggatctgg aacattacgc cttcaatatg
    24661 gaacgcttgc ggctcgtccg cgcgtaccgt ccagcgttcc cacgttcctt gctgcgcggt
    24721 gacatcaata cggttgacgt cccacagctg tccattaata cccgggtcat cacccagact
    24781 gatatcgcga ctgcgaattg gcgaaccagc catgatttca gttggcagca agcgcatcgg
    24841 aagactgtct gtgaccagcg gcagaaggcc ggttgggcgc agcgttagca ccagggtaga
    24901 aaccagaata ctggatggct caaagaagcc gcgaatacga tcaacaatgc tcgccgcttc
    24961 gccacaggtg atcgacactt catcgccgtt gctcatatcc accagaatct cgcggcgctc
    25021 gcccggtgcc agcgaaagtt gcttcaccga tacaggagca ggcaggaatc cctgatcgcc
    25081 agaaatcaca tgtaacgggc gaccatcgct catctgtagt tgatagcgac gagagttcga
    25141 cgcgttcagc agtcgcaagc gcacccagcc acgcgagact tcaacgtacg ggctttgtac
    25201 accgttaacc agcagcgtat caccaacaaa gccgccgctt cccggttcgt tgtattctgg
    25261 cgtaccaaag ttatccagcc gtttatcctg gataatgacc ggaaaatcat ccacaccata
    25321 atggttgggg ataggcagcg acttgctgac ttcatcttcc accagccaca ttccggcaag
    25381 gccgttatag acctgctgag ccgtgcggtt gggagtattg gcgtgatacc acagagtagc
    25441 tgcgttctgg cgaatgggca gtacgggtgc ccagtcagcg tttggcgaca tcatccgtgc
    25501 cggaccgccc atcagcgggc ctggtacctg tagcccggcc accgtcattg agacattttc
    25561 tgtcaggcgg ttgctgtaaa taagcttaac atcgtcgccc ttccagacgc ggatagtcgg
    25621 ccccaggtaa cgaccattga ttccccagac cgacgcgcgt gtccctggcg taaatgacca
    25681 gtgcgcacgt tgtacagtca taaacagcgg ttgcccacgg cgagattcaa gtagcggcgg
    25741 aacgggtagc ggttgctgtt gcccggctgc gctggccttc aggggaacag cgcctgcaca
    25801 aagtgcaatc cccgatgcct gaatgaactg acgccgactg agtgacatat ttgctccatg
    25861 taaaactggc taaagtaata ccgtgcagga aagtgttccc cgcaaaaata cagattgccg
    25921 acttaaactt ttccggcggc ttcgcgttct gcgacttctt tatcgagctc ggcgattttt
    25981 tgttccatta tcgaacgaca atgggcagcc agctcacgaa cctgatcttt gccatactga
    26041 ctgacgtcaa ttggcggcag catttcgaca atcaccagac cgttgtgcag tcgattaaga
    26101 ttaatcttat tcgaagttgt agagacgcac acgggaataa tcgggacgcc cgccgcaatt
    26161 gccgcgtgaa atgctccagt cttgaacggt agcaggccgc gaccacggct gcgggttcct
    26221 tccgggaaca tccagatgga aatacggcgt tttttgaaat gattcacgac ttccgcaatg
    26281 gtgccgtgag ctttagtgcg attgtttctg tcgatcaata agttgccggt taaccagtac
    26341 aactgcccga agaaggggat ccacagcaag ctctttttac ctaccgtcac cgtcggcggt
    26401 tgcacaatgt tcgatgctgt caccatgtca tagttgttct ggtggttagc gatatagata
    26461 gcattgccgt agctttcagc atctgcaggt ttgcggcact caactttcag gccgaacagc
    26521 ggcgcaagac ggccaaacat atgcccaaag gtggccacat gtttcgggtt acgcgggctg
    26581 aaaaggcagt aaatggagcc gaatacacag actaagatgc tgtaaatcac ggtaataata
    26641 agacgaaaga tatatagcat aacaccctct gaaggtctga gagactggca ttgtacgtca
    26701 cctgaaatca ggttagggca ttgttaaaag ccgctctctg gaaaagcgta ttgttaatcg
    26761 caacgcatga ataaacaacg gtttcaaggg aatttttatc gtttccgggg gttgaatacg
    26821 ctgccggatg acgacttaac gtttcatccg gcgttccttg caagcgggag gaaacaggcc
    26881 ctccccggca tattactctt cgctatcacc gctgctggca cggcgaggag agtcgatctc
    26941 aacacgatcg atacgctgca aaccgcgcat caacgtaccg cggcgtccac gttcgccagt
    27001 gactttctgt aactcttccg ggcgcagttt aattttgcgt ttcccaacat gaatggtcag
    27061 cgtgctttgc ggcggcagaa cgtacaattg cgccagacca tcttctccac gcgcggcttc
    27121 tgccgatgga atgttgataa tcttgttgcc tttgcccttc gacagctgcg gcagatcgct
    27181 taccgggaac atcaacatac ggcctgcctg agtgattgcc agcagcatat cggaagcatc
    27241 ttcaatcacc accggcggca taacatgggc attttccggt aaggtgatca aagccttacc
    27301 tgcacggtta cgcgccacca gatcgttaaa ggtgcagacg aaaccgtaac ccgcatcgga
    27361 agccatcagc agtttctgat cgtcgctttc catcagcata tggtcaacgg tcgccccagg
    27421 cggcaacgtt aatttgccgg tgagtggttc gccctgacca cgcgccgacg gcagcgtaat
    27481 cgggtcgatg gcatagctac gaccggtgga atcaacaaac actaccggtt ggttgctctt
    27541 acctttcacc gccgctttga agctatcacc cgctttataa ttcaggcccg gcgcgtcgat
    27601 atcatggcct ttagcgctgc gtacccagcc catctgcgac agcacaatgg tgacaggttc
    27661 agacggcagc atgtcgtgct cgctcatcgc tttcgcttct tcgcgttcct gcaacggcga
    27721 acgacgatcg tcaccgtagg cttgcgcgtc tgcctgcagt tctttcttca gcaggttatt
    27781 cattttacgc tcggaagcca aaatgccctg caactggtcg cgctcttttt ccagctcact
    27841 ctgctcaccg cgaatcttca tctcttccag tttggcaaga tgacgcagtt tcagttcgag
    27901 gatcgcttcc gcctgggttt ccgtaaggcc aaaccgcgac atcagcgccg gtttcggttc
    27961 atcttcgtta cgaatgatct caatcacttc gtcgatgttg agaaacgcca ccagcaaacc
    28021 ttccaggata tgcaggcgct tgaggacttt ctccagacga tagttcagtc ggcggcgcac
    28081 ggtatcgcga cggaacacca gccattcgga gaggatttcc agcaggtttt tcaccgccgg
    28141 acgaccatcc agaccgatca tattgaggtt aatgcgatag ctcttttcca gatcggtggt
    28201 agcgaagagg tggttcatca cctgatccat atccacgcgg ttggaacgcg gcacaatcac
    28261 cagacgggtc gggttctcgt ggtcagattc atcgcgcaga tcgtcaacca tcggcagctt
    28321 tttgttgcgc atttgcgcag caatttgctc cagtacgcgc gcacctgaaa cctgatgcgg
    28381 taatgcgctg ataaccaccg cgccatcttc tttcttccac accgcgcgca tacgcactga
    28441 accacgtccg ttctcgtaga ttttacggat ctcggcgcgc gaagtgataa tttccgcttc
    28501 agtcggataa tccggcccct gcacgatatc cagcagctga tcgagcgtgg ttttcggctg
    28561 gtcgattaat gcaattgccg cctgagctac ttcacgcagg ttatgcggtg gaatatcggt
    28621 cgccatgccg acggcaatac cggtggtgcc gttaagcaaa atgtttggca gacgagcagg
    28681 cagcattttc ggctcctgca aagtgccgtc gaagtttggc acccagtcag ccgtcccctg
    28741 ccccagctcg ctcaatagca gctcggaata tttcgacaac cgggattcgg tgtaacgcat
    28801 tgccgcgaac gatttcggat cgtccggcgc gccccagttc ccctgaccat caaccagcgg
    28861 ataacggtaa gagaacggct gcgccatcag gaccatcgct tcataacagg cgctatcgcc
    28921 gtgcggatgg tatttaccca gtacgtcacc gacggtacgg gccgattttt taaatttggc
    28981 gctggcattc aggcccagtt cagacatcgc atacacaatg cggcgctgaa caggtttaag
    29041 accatcgcca ataaacggca acgcacggtc catgatcacg tacatggagt agtttaagta
    29101 ggcgttttcc gtaaattcat gtagcgcaag gcgctctgcc atatcgctca ttaattctga
    29161 ttcctcaact tattcgccag ccttcaaagg ggcaatattg ccggagatac tacattatct
    29221 gccgcgtcga gtcacaggag atcagcgtga atagcgccgc cggacgtgct ccgacggcga
    29281 taaaggcatc aatgtgcctt gatatacaac tctttgctgt agacttgatc ggcatagagg
    29341 gcagcacaag gttgcgccca tgccacacca ctcggcatgg gggtccgctc cgggcgattc
    29401 gagaagttga ggttatgcga gtccagggaa gcctaaagca tcaaagtagt cgactgtaag
    29461 tatacggttc agcccagttt agctgtgtct ccaccagtgc cccgcgcctc ctgccataag
    29521 tgcggcttgc tcatggatac ccccgagacg gcttacggcg caccggataa taaatttcgc
    29581 gtatacatgg tattccttac cggagaaatt ataagcccaa caacaatacg cctgtggcat
    29641 tgttcgctca aacttatcgc gagtgatttg gctcacactc cggtaaagat tccccatcac
    29701 gccaacggca cataaacatc cgtttccagt cgctgctctg tcaccccttc ggcaagattg
    29761 gtgtagtgaa acagaatcgg tgctttacgc attttctcgc cgcttgcagg cagccagtgg
    29821 cgaataatgc cccatatcgt gtgtgaaata tcgtccagct cgccaacgtg gcgggccacg
    29881 gcataacgtc caccggtaag ttcaccattg ctcacaccat aacgattatc gggaatcggt
    29941 tcgctaacgc tgccgcagat atcaaagcga aacgcttccg gtgcggtggt ggcagggtcg
    30001 tcccaggcga cgccaaaagt ctggctttgg ttaacaggag aaagccccgt ctctttacgc
    30061 cacatgataa atttcgccac actgtagttg agcaactcag ggctacagcg gtgcggcaac
    30121 atcgccacag ggattgatgg gaaatcgata atgtttacat ccagtgtcag gtttgtcatt
    30181 ttgcctccct gcgaaattat tcccgggaac gcgtcccggg agcggtaaca attacggatt
    30241 tactttgcgg atctgtttga cgtcaatttc aacagaattc cagtctttat cgacttcacc
    30301 ctgaatctca accgtatctt tcggcgtcac cgtcacaccg ttccagcgtt tgtggtcgat
    30361 atcaacattg atagtaccgc tggcatcttt aaacacgtag agatcgtcag agatgcgttc
    30421 aacgatattg ccgcgcaggg ttacccaggt gtcgtcacgc agggattttg cgctttctac
    30481 ggtcgttacg ctgccgttcg gcccctggaa tcctccggcc tgactttgcg ttgccgatgg
    30541 gccagaaaaa ccgccctgct ctgctgccat caccggtgcg ctgcacaggg ccattactgc
    30601 gattactgct gcgaattttt tcatgtttat tactcccttt aatgtctgtt tccgagcatt
    30661 taacaagata gtccttaaca acttcttaag ggaaaaaaat aaaatttagt gctgtacaga
    30721 gcgcgttaca acacggttta ctggcagcaa atacggttat cgcagggatg aaaaaatgcg
    30781 aattttactg atagaagatg acatgctgat tggcgacggc atcaaaacgg gccttagtaa
    30841 aatgggtttt agcgtcgact ggtttacaca aggtcgtcag ggaaaagagg cgctttatag
    30901 cgcgccttat gatgcggtga tcctggattt aaccttacca ggcatggatg gtcgcgatat
    30961 tttgcgcgaa tggcgagaaa aaggtcagcg tgagccggta ctgatcctga ccgcgcgcga
    31021 tgcgctggag gaacgtgtag aagggctgcg tctgggagct gacgattatc tgtgtaaacc
    31081 ttttgcgttg atagaagtcg ccgccaggct ggaagctctg atgcgccgaa ccaacggcca
    31141 ggccagcaac gagctgcgcc acggtaacgt catgctcgac cccggcaaac gtatcgccac
    31201 gctggctggc gaacccttaa cactgaaacc aaaagaattt gccctgctgg aattactgat
    31261 gcgtaacgct ggtcgggtac tgccgcgcaa actgattgaa gagaaactgt atacctggga
    31321 cgaagaggtc accagtaatg ccgttgaagt gcatgtgcat catctgcgac gcaaactcgg
    31381 tagtgatttt attcgtaccg tgcatggtat tggctacaca ttaggtgaga aatgaaattt
    31441 acccaacgtc ttagtctgcg cgtcaggctg acgctaatct ttttaattct ggcctcggtg
    31501 acctggctgc tttccagctt tgtcgcctgg aaacaaacaa cggataacgt cgatgaattg
    31561 ttcgacaccc aactgatgct gtttgccaag cggttaagta cgctcgatct caacgaaatc
    31621 aacgcggcgg atcgcatggc acagacgcca aataaattaa aacacggtca tgttgatgac
    31681 gatgcgctga cctttgccat ctttacccac gacggcagaa tggtccttaa cgatggcgat
    31741 aacggagaag atattcctta tagctatcaa cgggaaggtt ttgctgacgg gcaactggtg
    31801 ggcgataaag atcaatggcg ttttgtctgg atgacctcac ctgatggcaa atatcgcatc
    31861 gttgttggtc aggaatggga ataccgtgaa gacatggcgc tggcgattgt tgccgggcaa
    31921 ttgatcccgt ggctggtcgc actgccgatt atgttaatca tcatgatggt actattgggt
    31981 cgtgaactcg cgccgctgaa caaactggcg ctggcgctgc gtatgcgtga ccctgactcg
    32041 gaaaaaccac taaacgcaac tggcgtaccc agcgaagtgc gtccgttggt tgagtcgcta
    32101 aatcaactgt tcgcccgcac acatgcgatg atggttcgtg aacgacgctt tacctccgac
    32161 gcagctcacg aactacgtag cccgttaacg gcgctgaaag tgcaaaccga agttgcgcag
    32221 ctttctgacg atgatccgca agcgcggaaa aaagcgctgc ttcaattaca ttacgggatc
    32281 gatcgcgcta ctcgtctggt tgatcaactg ctcactctat cgcggctgga ctcactggat
    32341 aaccttcagg acgtcgcgga gatcccgcgt gaagatctcc tgcaatcgtc ggtgatggat
    32401 atttaccaca cggcgcagca ggcgaaaatt gacgtgcgac tgacactcaa tgcccacggc
    32461 atcaaacgca ccggacaacc gctattgcta agtttgttgg tgcgaaattt gctggataac
    32521 gccgtgcgct acagtccaca gggcagcgtg atagacgtca cgctgaatgc tgataatttc
    32581 atcgtgaggg ataacggccc cggtgtgacg ccagaggctc tggcgcgaat tggcgaacgc
    32641 ttctatcgcc cccccggaca aaccgctacc ggcagtggac ttgggctatc gattgtccag
    32701 cgaatcgcca aattgcatgg catgaatgtt gagtttggga atgcggaaca aggtggattt
    32761 gaggcgaagg taagctggta acttctctgt ctatgagagc cgttaaaacg actctcatag
    32821 attttactaa tagcaaaata taaaccgtcc ccaaaaaagc caccaaccac aaaccagaca
    32881 aataccgagc gaaaatacat atacccaaaa ctcgaacatt tcccgcataa agagttttct
    32941 taagataaga ataataagtg gcgtaagaag aaaaaatgct gcaatcattg cttgcaatat
    33001 acgatcttcg cctgaaacgg aaggtatact gcaaatattg tctcccgact caagcatcca
    33061 gtcatactta ttgaaagcaa aaaaagcatc tattaaccaa taagtaagca ttatgtataa
    33121 cagagcttca aatattg
//
LOCUS       DAZFQD010000041        32605 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.041, whole genome shotgun sequence.
ACCESSION   DAZFQD010000041 DAZFQD010000000
VERSION     DAZFQD010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32605
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.041"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(145..891)
                     /gene="cutC"
                     /locus_tag="ACHKTW_002029"
     CDS             complement(145..891)
                     /gene="cutC"
                     /locus_tag="ACHKTW_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFO6463057.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLYTLNNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DEHADEYSRYIVDGAAVAEMKGIIERHQAK"
     gene            complement(905..1471)
                     /gene="yecM"
                     /locus_tag="ACHKTW_002030"
     CDS             complement(905..1471)
                     /gene="yecM"
                     /locus_tag="ACHKTW_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFO6463058.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSLRLGLNITPLTADHIS
                     LRCHQNATAERWRRGFEQCGELLSENMINGRPICLFKLHEPVQFAHWQFSIVELPWPG
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            1687..3420
                     /gene="argS"
                     /locus_tag="ACHKTW_002031"
     CDS             1687..3420
                     /gene="argS"
                     /locus_tag="ACHKTW_002031"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFO6463059.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQVRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVSTPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDAAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYESLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEINEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            complement(3462..3506)
                     /gene="yecV"
                     /locus_tag="ACHKTW_002032"
     CDS             complement(3462..3506)
                     /gene="yecV"
                     /locus_tag="ACHKTW_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YecV"
                     /protein_id="HFO6463060.1"
                     /translation="MSIFRIHLDGNKKA"
     gene            3597..4085
                     /gene="yecT"
                     /locus_tag="ACHKTW_002033"
     CDS             3597..4085
                     /gene="yecT"
                     /locus_tag="ACHKTW_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416391.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HFO6463061.1"
                     /translation="MFKFLVLTLGIISCQAYAEDTVIVNDHDISAIKDCWQKNSDDDT
                     DINVIKSCLRQEYNLVDAQLNKAYGEAYRYIEQVPRTGVKKPDTEQLNLLKKSQRAWL
                     DFRDKECELILSNEDVQDLSDPYSESEWLSCMIIQTNTRTRQLQLYRNSEDFYPSPLT
                     RG"
     gene            complement(4205..4597)
                     /gene="flhe"
                     /locus_tag="ACHKTW_002034"
     CDS             complement(4205..4597)
                     /gene="flhe"
                     /locus_tag="ACHKTW_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HFO6463062.1"
                     /translation="MRTLLAILLFPLLVQAAGEGMWQASSVGITLNHRGESMSSAPLS
                     TRQPASGLMTLVAWRYQLIGPTPSGLRVRLCSQSRCVELEGQSGTTVAFSGIAAAEPL
                     RFIWEVPGGGRLIPPLKVQRNEVIVNYR"
     gene            complement(4597..6676)
                     /gene="flhA"
                     /locus_tag="ACHKTW_002035"
                     /pseudo
     CDS             complement(4597..6676)
                     /gene="flhA"
                     /locus_tag="ACHKTW_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416393.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            complement(6669..7817)
                     /gene="flhB"
                     /locus_tag="ACHKTW_002036"
     CDS             complement(6669..7817)
                     /gene="flhB"
                     /locus_tag="ACHKTW_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HFO6463063.1"
                     /translation="MSDESDDKTEAPTPHRLEKAREEGQIPRSRELTSLLILLVGVSV
                     IWFGGVSLARRLSGMLSAGLHFDHSIINDPNLILGQIILLIREAMLALLPLISGVVLV
                     ALISPVMLGGLVFSGKSLQPKFSKLNPLPGIKRMFSAQTGAELLKAILKTILVGSVTG
                     FFLWHHWPQMMRLMAESPITAMGNAMDLVGLCALLVVLGVIPMVGFDVFFQIFSHLKK
                     LRMSRQDIRDEFKQSEGDPHVKGRIRQMQRAAARRRMMADVPKADVIVNNPTHYSVAL
                     QYDENKMSAPKVVAKGAGLVALRIREIGAENNVPTLEAPPLARALYRHAEIGQQIPGQ
                     LYAAVAEVLAWVWQLKRWRLAGGQRPVQPTHLPVPEALDFINEKPTHE"
     gene            complement(8019..8663)
                     /gene="cheZ"
                     /locus_tag="ACHKTW_002037"
     CDS             complement(8019..8663)
                     /gene="cheZ"
                     /locus_tag="ACHKTW_002037"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0050920 - regulation of chemotaxis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HFO6463064.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTRMLRDSLRELGLDQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGQVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKAGVVASQDQVDDLLDSLGF"
     gene            complement(8674..9063)
                     /gene="cheY"
                     /locus_tag="ACHKTW_002038"
     CDS             complement(8674..9063)
                     /gene="cheY"
                     /locus_tag="ACHKTW_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HFO6463065.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(9078..10127)
                     /gene="cheB"
                     /locus_tag="ACHKTW_002039"
     CDS             complement(9078..10127)
                     /gene="cheB"
                     /locus_tag="ACHKTW_002039"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HFO6463066.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELSRSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLAKISAGQAIRI"
     gene            complement(10130..10990)
                     /gene="cheR"
                     /locus_tag="ACHKTW_002040"
     CDS             complement(10130..10990)
                     /gene="cheR"
                     /locus_tag="ACHKTW_002040"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HFO6463067.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRSLGLTDFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHARRRSGEYRVWSAAASTGEEPYSIAMTLADTLGTAPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQNTQQEILRRFVPLLKPDGLLFAGHSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(11009..12610)
                     /gene="tap"
                     /locus_tag="ACHKTW_002041"
     CDS             complement(11009..12610)
                     /gene="tap"
                     /locus_tag="ACHKTW_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein IV"
                     /protein_id="HFO6463068.1"
                     /translation="MFNRIRISTTLFLILILCGILQIGSNGMSFWAFRDDLQRLNQVE
                     QSNQQRAALAQTRAVMLQASTALNKAGTLTALSYPADDIKTLMTTARASLTQSTTLFK
                     SFMAMTAGNEHVRALQKETEKSFARWHNDLEHQATWLESNQLSDFLTAPVQESQNAFD
                     VNFEAWQLEINHVLEAASAQSQRNYQISALVFISMIIVAAIYISSALWWTRKMIVQPL
                     AIIGSHFDSIAAGNLARPIAVYGRNEITAIFASLKTMQQALRGTVSDVRKGSQEMHIG
                     IAEIVAGNNDLSSRTEQQAASLAQTAASMEQLTATVGQNADNARQASELAKNAATTAQ
                     AGGVQVSTMTHTMQEIATSSQKIGDIISVIDGIAFQTNILALNAAVEAARAGEQGRGF
                     AVVAGEVRNLASRSAQAAKEIKGLIEESVNRVQQGSKLVNNAAATMIDIVSSVTRVND
                     IMGEIASASEEQQRGIEQVAQAVSQMDQVTQQNASLVEEAAVATEQLANQADHLSSRV
                     AVFTLEEHEVARHESAQLQIAPVVS"
     gene            complement(12656..14317)
                     /gene="tar"
                     /locus_tag="ACHKTW_002042"
     CDS             complement(12656..14317)
                     /gene="tar"
                     /locus_tag="ACHKTW_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein II"
                     /protein_id="HFO6463069.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNIDEKYKNYYTALTELIDYLDYGNTGAYFAQPTQGMQNA
                     MGEAFAQYALSSEKLYRDIVTDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLT
                     PLAKIIAHIREIAGGNLANTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIY
                     AGTREIAAGNTDLSSRTEQQASALEETAASMEQLTATVKQNADNARQASQLAQSASDT
                     AQHGGKVVDGVVKTMHEIADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVAGEVRNLASRSAQAAKEIKALIEDSVSRVDTGSVLVESAGETMNNIVNAVTRV
                     TDIMGEIASASDEQSRGIDQVALAVSEMDRVTQQNASLVQESAAAAAALEEQASRLTQ
                     AVSAFRLAASPLTNKPQTPSRPASEQPPAQPRLRIAEQDPNWETF"
     gene            complement(14462..14965)
                     /gene="cheW"
                     /locus_tag="ACHKTW_002043"
     CDS             complement(14462..14965)
                     /gene="cheW"
                     /locus_tag="ACHKTW_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HFO6463070.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(14986..16950)
                     /gene="cheA"
                     /locus_tag="ACHKTW_002044"
     CDS             complement(14986..16950)
                     /gene="cheA"
                     /locus_tag="ACHKTW_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HFO6463071.1"
                     /translation="MSMDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASFDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRSQSPR
                     RIILSRLKAGEVDLLEEELGHLTTLTDVVKGADSLSAILPGDIAEDDITAVLCFVIEA
                     DQITFETVEVSPKISTPPVLKLAAEQAPTGRVEREKTTRSSESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSTAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQGTGTTIRILLPLT
                     LAILDGMSVRVADEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(16955..17881)
                     /gene="motB"
                     /locus_tag="ACHKTW_002045"
     CDS             complement(16955..17881)
                     /gene="motB"
                     /locus_tag="ACHKTW_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HFO6463072.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEELKKRMEQSRLRKLRGDLDQLIESDPKLRALRPHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRAIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLDSGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            complement(17878..18765)
                     /gene="motA"
                     /locus_tag="ACHKTW_002046"
     CDS             complement(17878..18765)
                     /gene="motA"
                     /locus_tag="ACHKTW_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HFO6463073.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRILADSVMLDFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGVVHALGSADRPAAELGALIAHAMVGTFLGILLAYG
                     FISPLASVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQTTTEEA"
     gene            complement(18892..19470)
                     /gene="flhC"
                     /locus_tag="ACHKTW_002047"
     CDS             complement(18892..19470)
                     /gene="flhC"
                     /locus_tag="ACHKTW_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HFO6463074.1"
                     /translation="MSEKSIVQETRDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(19473..19823)
                     /gene="flhD"
                     /locus_tag="ACHKTW_002048"
     CDS             complement(19473..19823)
                     /gene="flhD"
                     /locus_tag="ACHKTW_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HFO6463075.1"
                     /translation="MHTSELLKHIYDINLSYLLLAQRLIVQDKASAMFRLGINEEMAT
                     TLAALTLPQMVKLAETNQLVCHFRFDSHQTITQLTQDSRVDDLQQIHTGIMLSTRLLN
                     DVNQPEEALRKKRA"
     gene            20603..21031
                     /gene="uspC"
                     /locus_tag="ACHKTW_002049"
     CDS             20603..21031
                     /gene="uspC"
                     /locus_tag="ACHKTW_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspC"
                     /protein_id="HFO6463076.1"
                     /translation="MSYSNILVAVAVTPESQQLLAKAVSIARPVKGHISLITLASDPE
                     MYNQLAAPMLEDLRSVMQEETQSFLDKLIQDAGYPVDKTFIAYGELSEHILEVCHKHH
                     FDLVICGNHNHSFFSRASCSAKRVIASSEVDVLLVPLTGD"
     gene            complement(21038..22462)
                     /gene="otsA"
                     /locus_tag="ACHKTW_002050"
     CDS             complement(21038..22462)
                     /gene="otsA"
                     /locus_tag="ACHKTW_002050"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase"
                     /protein_id="HFO6463077.1"
                     /translation="MSRLVVVSNRIAPPDEHAASAGGLAVGILGALKAAGGLWFGWSG
                     ETGNEDQPLKKVKKGNITWASFNLSEQDLDEYYNQFSNAVLWPAFHYRLDLVQFQRPA
                     WDGYLRVNALLADKLLPLLQDDDIIWIHDYHLLPFAHELRKRGVNNRIGFFLHIPFPT
                     PEIFNALPTYDTLLEQLCDYDLLGFQTENDRLAFLDCLSNLTRVTTRSAKSHTAWGKA
                     FRTEVYPIGIEPKEIAKQAAGPLPPKLAQLKAELKNVQNIFSVERLDYSKGLPERFLA
                     YEALLEKYPQHHGKIRYTQIAPTSRGDVQAYQDIRHQLENEAGRINGKYGQLGWTPLY
                     YLNQHFDRKLLMKIFRYSDVGLVTPLRDGMNLVAKEYVAAQDPANPGVLVLSQFAGAA
                     NELTSALIVNPYDRDEVAAALDRALTMSLAERISRHAEMLDVIVKNDINHWQECFISD
                     LKQIVPRSAESQQRDKVATFPKLA"
     gene            complement(22437..23237)
                     /gene="otsB"
                     /locus_tag="ACHKTW_002051"
     CDS             complement(22437..23237)
                     /gene="otsB"
                     /locus_tag="ACHKTW_002051"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HFO6463078.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDISVQLHTVIAQYPGAELEAKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITTALQQKRENNRSDDYESFSRSI"
     gene            complement(23404..24390)
                     /gene="araH"
                     /locus_tag="ACHKTW_002052"
     CDS             complement(23404..24390)
                     /gene="araH"
                     /locus_tag="ACHKTW_002052"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HFO6463079.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGILCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGVPVV
                     RTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKI
                     SYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
     gene            complement(24405..25919)
                     /gene="araG"
                     /locus_tag="ACHKTW_002053"
     CDS             complement(24405..25919)
                     /gene="araG"
                     /locus_tag="ACHKTW_002053"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP-binding protein
                     AraG"
                     /protein_id="HFO6463080.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLSIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIDNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQLRSYGEERLRLDAVKAPGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGLFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     PCPEDRKAEGIIPVHSVRDNINISARRKHVLGGCVINNGWEENNADHHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRGIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            complement(25989..26978)
                     /gene="araF"
                     /locus_tag="ACHKTW_002054"
     CDS             complement(25989..26978)
                     /gene="araF"
                     /locus_tag="ACHKTW_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
                     /protein_id="HFO6463081.1"
                     /translation="MHKFTKALAAIGLAAVMSQSAMAENLKLGFLVKQPEEPWFQTEW
                     KFADKAGKDLGFEVIKIAVPDGEKTLNAIDSLAASGAKGFVICTPDPKLGSAIVAKAR
                     GYDMKVIAVDDQFVNAKGKPMDTVPLVMMAATKIGERQGQELYKEMQKRGWDVKESAV
                     MAITANELDTARRRTTGSMDALKAAGFPEKQIYQVPTKSNDIPGAFDAANSMLVQHPE
                     VKHWLIVGMNDSTVLGGVRATEGQGFKAADIIGIGINGVDAVSELSKAQATGFYGSLL
                     PSPDVHGYKSSEMLYNWVAKDVEPPKFTEVTDVVLITRDNFKEELEKKGLGGK"
     gene            27775..28278
                     /gene="ftnB"
                     /locus_tag="ACHKTW_002055"
     CDS             27775..28278
                     /gene="ftnB"
                     /locus_tag="ACHKTW_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HFO6463082.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(28357..28608)
                     /gene="yecJ"
                     /locus_tag="ACHKTW_002056"
     CDS             complement(28357..28608)
                     /gene="yecJ"
                     /locus_tag="ACHKTW_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 family protein YecJ"
                     /protein_id="HFO6463083.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTNHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(28723..28809)
                     /gene="azuC"
                     /locus_tag="ACHKTW_002057"
     CDS             complement(28723..28809)
                     /gene="azuC"
                     /locus_tag="ACHKTW_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HFO6463084.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            29072..29395
                     /gene="yecR"
                     /locus_tag="ACHKTW_002058"
     CDS             29072..29395
                     /gene="yecR"
                     /locus_tag="ACHKTW_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HFO6463085.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGIADRACQQEGYTHAVPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
     gene            29566..30063
                     /gene="ftnA"
                     /locus_tag="ACHKTW_002059"
     CDS             29566..30063
                     /gene="ftnA"
                     /locus_tag="ACHKTW_002059"
                     /EC_number="1.16.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HFO6463086.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(30101..30340)
                     /gene="yecH"
                     /locus_tag="ACHKTW_002060"
     CDS             complement(30101..30340)
                     /gene="yecH"
                     /locus_tag="ACHKTW_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family metal-binding protein"
                     /protein_id="HFO6463087.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGMTAGELVAFLAAKGKFIPSKDGFSTDQSKICRH"
     gene            30531..31742
                     /gene="tyrP"
                     /locus_tag="ACHKTW_002061"
     CDS             30531..31742
                     /gene="tyrP"
                     /locus_tag="ACHKTW_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HFO6463088.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNVRKLRWVF
                     ITGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(31804..32469)
                     /gene="yecA"
                     /locus_tag="ACHKTW_002062"
     CDS             complement(31804..32469)
                     /gene="yecA"
                     /locus_tag="ACHKTW_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HFO6463089.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIEPEQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
BASE COUNT         8001 a         8661 c         7758 g         8185 t
ORIGIN      
        1 ctatgttcac tgtcacccgc tcaacagatc acggttatcg ttcgtttttt atactgctca
       61 gggataaaaa aaggcccctg ttgaaattgc aggggcctgg tacgagcaag catcatattg
      121 ggcgacatga tgcaacggta aaaatcattt ggcctgatgg cgttcaatga ttcctttcat
      181 ttcagcaacc gccgccccgt ctacgatata acgcgaatac tcgtccgcgt gttcatctga
      241 tgacatggac aatccttgat tacgataacg catcggtgag gcttgccacg ctcccgcgga
      301 gctatggact tccagcactc cggcatcgag gaagtggtgc aggttttctg cacggactcc
      361 tgctccggcc ataatgattg gagcatcacg atgggcaata agttccataa tttttgataa
      421 accttgcagt gcgtctgatt tttgccctga tgtcagtacc cgggcaatgc ccaattccgc
      481 aagattattg agtgtatata aagggttagc gcacatatcg aaggcgcgat gaaaagtcac
      541 tgccagcgga ccggcagcag ccattatttt ttccattcgt ggcatatcga cattcccgtc
      601 aacatcgaga acgcccgtca ccagtccagg aaaacctaat tcgcggaccg tgcgcacatc
      661 ctcaagaatg gcggcaaact caccgtcgct gtagcaaaaa tcaccaccgc gtgggcgaat
      721 tatcggatgc acagggatcg tcacccgctg gcgcacggat ttcagtaccc ccaacgacgg
      781 cgttaagccc ccctcttttg gggctgcgca taattcaact ctgtctgcgc cgttttgctg
      841 cgccgttagt gcacattcca tgctgtaaca gcaaatttcc agtaatgcca tttttgctcc
      901 ttaattacgc tgactgctcg ctggcaacga tctcttcaat ggaccacgga tgaaacttaa
      961 tagttgtttt gccatcggtg accgccagcg tcggattcgg taatcgctca tgctcacctt
     1021 ttggtgaact ggttttaact gagatccccg gtaggctgag tccttcatca gaaagcaatg
     1081 ccagcgcacg ggcattcagc gtttctggat cacccggcag aacgatttca atatgttccc
     1141 aaccttcgtg tgggtaacgt ttttccccgg gccacggtag ctccacaata gaaaactgcc
     1201 agtgcgcaaa ctgtaccggt tcatgcaatt taaacagaca aatcggtctg ccattgatca
     1261 tattttctga caaaagctcg ccacactgtt caaacccgcg acgccagcgt tcagcagtgg
     1321 cgttttgatg gcaacgcaaa gaaatgtgat cggcagtcag cggagtgata ttcaacccca
     1381 gacggaggga aagttcatct aatgcgtggg taaatcgcgg taaatccgat gcaatatcct
     1441 gcagctcgtc gatagattgc cagttcgcca taatcactct tcgtctttca gtaaaagcgt
     1501 taatttaccc tgttgccctg cgccaaccaa ccgctgattt cacgccgctt ctgatgcaat
     1561 agtgaaaacg gcaatacgcc acgcgcacgt tgctgacgaa aacagccatt tgcagtatac
     1621 tcccgcccta atttctttaa ctggtgcggg caatttttgc tcgcttcatc aatgtaaggt
     1681 attccggtga atattcaggc tcttctctca gaaaaagtcc gtcaggccat gattgcggca
     1741 ggcgcgcctg cggattgcga accgcaggtt cgtcagtcag caaaagttca gttcggcgac
     1801 tatcaggcta acggcatgat ggcagttgct aaaaaactgg gtatggcacc gcgacaatta
     1861 gcagagcagg tgctgactca tctggatctt aacggtatcg ccagcaaagt tgagatcgcc
     1921 ggtccaggct ttatcaacat tttccttgat ccggcattcc tggctgaaca tgttcagcag
     1981 gcgctggcgt ccgatcgtct cggtgtttct acgccagaaa aacagaccat tgtggttgac
     2041 tactctgcgc caaacgtggc gaaagagatg catgtcggtc acctgcgctc taccattatt
     2101 ggtgacgcag cagtgcgtac tctggagttc ctcggtcaca aagtgattcg cgcaaaccac
     2161 gtcggcgact ggggcactca gttcggtatg ctgattgcat ggctggaaaa gcagcagcag
     2221 gaaaacgccg gtgaaatgga gctggctgac cttgaaggtt tctaccgcga tgcgaaaaag
     2281 cattacgacg aagatgaaga gttcgccgag cgtgcacgta actacgtggt aaaactgcaa
     2341 agcggtgacg aatatttccg cgagatgtgg cgcaaactgg tcgacatcac catgacgcag
     2401 aaccagatca cttacgatcg tctcaacgtg acgctgaccc gtgatgacgt gatgggcgaa
     2461 agcctctaca acccaatgct gccaggaatt gtggcggatc tcaaagccaa aggtctggca
     2521 gtagaaagcg aaggggcgac cgtcgtattc cttgatgagt ttaaaaacaa ggaaggcgaa
     2581 ccgatgggcg tgatcattca gaagaaagat ggcggctatc tctacaccac cactgatatc
     2641 gcctgtgcga aatatcgtta tgaatcactg catgccgatc gcgtgctgta ttacatcgac
     2701 tcccgtcagc atcaacacct aatgcaggca tgggcgatcg tccgtaaagc aggctatgta
     2761 ccggaatccg taccgctgga acaccacatg ttcggcatga tgctgggtaa agacggcaaa
     2821 ccgttcaaaa cccgcgcggg tggtacagtg aaactggccg atctgctgga tgaagccctg
     2881 gaacgtgcac gccgtctggt ggcagaaaag aacccggata tgccagctga cgagctggaa
     2941 aaactggcta acgcggttgg tattggtgcg gtgaaatatg cggatctctc caaaaaccgc
     3001 accacggact acatcttcga ctgggacaac atgctggcgt ttgagggtaa taccgcgcca
     3061 tacatgcagt atgcttacac gcgtgtattg tccgtgttcc gtaaggcaga aattaacgaa
     3121 gagcaactgg ctgcagctcc ggtaatcatc cgtgaagatc gtgaagcgca actggcagct
     3181 cgcctgctgc agtttgaaga aaccctcacc gtggttgccc gtgaaggcac gccgcatgtg
     3241 atgtgtgctt acctgtacga tctggctggt ctgttctctg gcttctacga gcactgcccg
     3301 atcctcagcg cagaaaacga agaagtgcgt aacagccgtc tgaaactggc acaactgaca
     3361 gcgaagacgc taaagctggg tctggatacg ctgggtattg agacagtaga gcgtatgtaa
     3421 tcgatttttc gtgagagtaa agcctgatca gggttacctg atcaggcttt tttattgcca
     3481 tctaaatgta ttctgaaaat ggacatgcca ttgttttctg actgttggat aagaggccag
     3541 aagcgtaata tccggcccca gggaaacgat aacggttgaa tttaaggaat accgcagtgt
     3601 ttaaatttct tgtattaaca ttaggcatta tctcttgcca ggcttacgca gaagatacgg
     3661 ttatagtaaa cgaccatgac atttcagcca tcaaagattg ttggcaaaaa aattcagatg
     3721 atgatactga cattaacgtg atcaaatcat gcctgcgaca agaatacaat ctcgtcgatg
     3781 cgcaattaaa taaagcctat ggtgaagctt atcgttatat agaacaagtg ccacgcacag
     3841 gtgtaaaaaa acctgatacc gaacaactta acttgcttaa aaaatcacag cgagcctggc
     3901 tggattttag ggacaaagaa tgtgaattaa tcctttcaaa tgaggacgtt caggatttaa
     3961 gtgaccctta ttctgaatca gaatggctct catgtatgat catacagacc aatacgcgaa
     4021 ctcgccagtt gcagctatac cgtaactctg aagattttta tccaagccct ttgacaagag
     4081 gataattcac atctttttgg catgttttgt tgcaagctat tcctgataaa taattgcaac
     4141 aagacatcga gcctttttca ctgagttatt aaacatactc gcgagcgcgt aatttttttg
     4201 tccttcagcg ataattcaca atcacttcat tacgttgtac ctttagcggt ggaattaacc
     4261 gcccaccgcc tggcacttcc cagataaatc gcaacggttc tgctgccgct ataccggaaa
     4321 aggccacggt ggttccgctc tgcccctcta attcgacaca acgagattgc gaacacaagc
     4381 gaacccgcag tcctgaaggt gtcgggccga taagctgata acgccacgct accagcgtca
     4441 tcaatcctga agcaggttgt cgcgtagaaa gaggcgcaga cgacatcgac tcaccgcgat
     4501 gatttagcgt aattcctaca ctacttgcct gccacatccc ctccccggcg gcttgcacca
     4561 gcagcggaaa caataatatt gctaataagg ttctcattat ttgccgccaa ttgtcgccgt
     4621 catgcggata tgtcggttat cagacagttc cagattcgac aggaccacta actgcggcaa
     4681 gctgcggcgc aggaagcgag acaataatgg tcgcagcgcg tggttcacca acaatactgg
     4741 cggcgcaccc agcatctcct gacgggatag cgcttcctga gtttgcgcca gtaaacgatc
     4801 cgccagccct ggctccagtc ctcccccgcc ctgcagcgcc tgtagtaaca aacgttccag
     4861 cggtgtatcg aggccaataa catggacttc atctttgcca ggaaaccact gctgggtaat
     4921 cgcccgtccc aacgccacgc gcacgacggc ggttaattca tgtggatcgc tttggatggg
     4981 cgcatgttcc gccagcgttt cgagaatggt gcgcatatcg cgaatcggta ctttttcatc
     5041 gaggatgatt ttgcagcact ttatgcagtg tggtgagcgt gacgacgcca ggaacgagat
     5101 cttccgtcag ctttggcatc tcctgggcga cgcgatccaa cagctgttgc gcctcctgac
     5161 gaccaaacag ctctgcggca tgctggctaa tgaggtggtt aagatgcgtt gctaccaccg
     5221 tgctggcctc aaccactgtg tacccctgaa tctgcgcctg ttcttttagc gcactttcaa
     5281 tccagatagc attcaggcca aatgccggat cgacggtcgc ctcaccaggt aacgtcccgg
     5341 cagcggttcc agggttaatc gccagccagc gccccggata agcatcacca ctgccaatct
     5401 ccacgccttt catcaaaatg cgatagcggg caggttgcag atccatattg tcgcgaatgt
     5461 gcaccactgg cggcagaaat cccatctcct gggcaaattt cttgcggata ctgcgtatac
     5521 ggcccaacaa ctcaccatcc tgctggaaat cgaccatcgg gatcagtcga taacccactt
     5581 ccattcccag agaatcttcc agttgtacat cgttccacgt cgcttcgaca acggtattat
     5641 tctctgccat ttttaccggt ttgggttcga caggcgcttt ttgttcgcgt ccgcgtatcc
     5701 accaggccag ccccagcaat ccggcagtga acagcaaaaa taccaggttc ggcattccag
     5761 gcaccaggcc gagtaaaccg agcacggcgg cgcttaacaa cataacgctt gggttactga
     5821 aaagctgatt caccatctgc tcgccaacat cctgatcggt gctgacacgc gtaacgatga
     5881 cccccgcggc ggtagaaatc accagcgccg gaatttgtgc caccagaccg tcgccaatgg
     5941 tcaatagtgt ataactttcc gccgcgtgtc ccatgctcat gccatgttgc agcacgccga
     6001 ccagcaaccc gccgacaacg ttaatgacca tgatgaggat cccggcgatg gcatcgccgc
     6061 gaacaaactt acttgccccg tccattgagc cgtaaaaatc ggcttcctga gtcacttcgg
     6121 agcggcgttt tttcgcctca tcttcaccaa tcaatccagc gttaaggtcg gcgtcaatcg
     6181 ccatctgctt acccggcata ccatcgagaa caaagcgcgc acccacttct gcgatacgcc
     6241 cggcaccttt ggtaatgacc ataaagttga tgatcacgag aatgacaaac actacgatac
     6301 cgatagcgaa attgccacca acgaggaagt gaccgaatgc ttcgaccacc ttcccagccg
     6361 ccgccgcgcc ggtatgccct tccatcaaaa tgatacgggt tgaagccacg ttaagtgcca
     6421 gacgcaacaa cgtagtaaac aacagaatgg tcggaaacgc agcaaactca agcgtgcgct
     6481 gggtaaacat cgccaccagc aacaccatga tcgacaaggc aatattgaag gtaaacaaca
     6541 ggtcgagtat gaatgcgggc agtggcagca ccatcatcga caagatcaac aggatcaaaa
     6601 tcggtccggc aaggatctgc cattgtgtcg atttcaggtt tgcgggcagg cgcagcatcg
     6661 cggccagatt actcatgggt cggtttctcg ttaataaaat ccagggcttc cggcaccgga
     6721 agatgagtag gttgtacagg gcgctgtcca ccagccagac gccagcgttt cagttgccag
     6781 acccaggcca gcacttccgc caccgcggcg tacagttgac ccgggatttg ttgaccaatc
     6841 tccgcatgtc gatacagcgc tcgcgccagc ggcggcgctt caagcgtcgg gacgttattt
     6901 tcagcgccaa tttcacgaat gcgcagcgcg accagccctg cacctttagc gaccactttc
     6961 ggtgcgctca ttttgttttc gtcatactgc aacgctaccg aatagtgggt cgggttattg
     7021 acaatgacat ccgctttcgg cacatcggcc atcatccgac gccgtgcagc agctcgctgc
     7081 atctgacgga tccgcccttt aacatgaggg tcaccttcgc tttgtttgaa ctcatcacga
     7141 atatcctgcc gtgacatacg cagctttttc aggtggctga atatttggaa aaagacgtca
     7201 aatcccacca ttggaatgac accaagcacc accagcagtg cgcatagccc taccaaatcc
     7261 atcgcattac ccatggcggt aatcggagac tcggccatca agcgcatcat ctgcggccag
     7321 tgatgccaga gaaaaaaccc cgtcacgctg ccaaccagga tggttttcaa aattgcttta
     7381 agcaactccg cgccagtctg agccgagaac atccgtttaa tgcccggtag cgggttgagt
     7441 ttggaaaact tcggctgcaa ggatttgccg ctaaatacca gccctcccag catgaccgga
     7501 gaaatgagcg ccaccagcac cacgccgcta atcagcggca gcagcgccag catggcttct
     7561 ctgatcagca gaataatctg cccgaggatc agattcgggt cattgataat actgtgatca
     7621 aaatgcagcc cagcggagag catgcccgac aatcgacggg ccagcgacac accgccaaac
     7681 cagataacac taacgcccac cagcaaaatc agcagtgagg tcagttcacg ggaacgcggg
     7741 atttgcccct cttcccgcgc tttttctagt cggtgaggtg tgggggcttc tgttttgtcg
     7801 tcgctctcgt cagacacgtc gccaatcctg gaaagagtat taagcgtgaa tgatgccaga
     7861 gcgcaaagcg ttcaatggtt tgagtaaggg gcaaaacagg cgggatttag ggcttttgct
     7921 gccacacatc aagcatagtg tgcgtttgtc ggatgcggcg tcatcgcctt atcagaccgc
     7981 ctgatatgac gtggtcacgc cacatcaggc aatacaaatc aaaatccaag actatccaac
     8041 aaatcgtcca cctgatcctg actggctacc acaccggctt tgctggtatc gacctgaggt
     8101 ccattaagca aactctggtt ttcacgtttt ggacgcgact cctgttccgg gatgttttcc
     8161 aacagcacca tcagcaactg gcgttcgatc tcctgaatga catccatcat ccgcttaatg
     8221 acctgcccgg tgagatcctg aaaatcctgc gccatcatga tttccagcag ttgcgcgtta
     8281 gtaaagctgg tatgcgcggg tacatctgcc agaaattgtc gtgtatctgt taccagttca
     8341 cgggcgtcgg caaggtcaat cggatcggca aaccagtcat cccaacgttg ggttaacgct
     8401 tttgctgatt tctccatttg atcctgatgc ggttgtgacg cctcaacact gttcagcgcc
     8461 cgctccgcag cctgggcggt catctgcaca acatagtaca aacgatcgcg cgcatcgggg
     8521 atggcttccg ccgcttcggc aatggcctga tccagcccca gttcccgcaa actgtcgcgc
     8581 agcatacgcg tcaggctgcc gatgcgcgca atgatatcgc cagctgaatg ctcgtcagca
     8641 ggtttgattg atggttgcat catagtcgca tcctcacatg cccagtttct caaagatttt
     8701 gttgagtttt tcctccagcg tcgcggcggt aaatggcttc accacatagc cactggcccc
     8761 cgcttgcgcc gcagcaatga tgttctcttt cttcgcttct gcagtcacca ttaacactgg
     8821 caatgccgac atcgcgccat ccgcacgaat tgttttcagc aattccaggc catccatatt
     8881 gggcatgttc cagtcggaga taacaaatcc ataaccgcct gcctgcaact tattgagagc
     8941 gtcgacgcca tcttccgctt cctcaacatt attgaatccc agctctttca gcaggttacg
     9001 cactatgcgt cgcatggtgg aaaagtcatc cacaaccaaa aatttaagtt ctttatccgc
     9061 catttcacac tcctgattta aatacgtatc gcctgtccgg cactaatttt tgccaacatt
     9121 tgctggctta cctggctaag atcgaccact tcgcagacac cacccatatt gatggcctcg
     9181 cgcggcatgc cgaacaccac gcaacttgct tcgttttgcg caagggtcca tgcccccgcc
     9241 tgacgcatcg ccaacattcc cgccgcgccg tcgttgccca taccggtcag gatcacccca
     9301 accgcattac gccccgcctg tttggcgaca gaatggaaca acacatctac cgaaggccga
     9361 tgacggttaa ccgccgggcc atcgtgaatt ttgatttggt aatttgcgcc actacgcgac
     9421 agctccatat gccgatcgcc cggcgcgata taggcatgtc ccggcaaaac acgttctccg
     9481 tcttcggctt ctttaacccc gatctgacaa agcttattaa gtctgtcggc aaaagagcgg
     9541 gtgaaaccag gcggcatatg ctgggtaatt aacagtgccg ggctggaaag cggcaacggt
     9601 tgcagtacgt gacgaattgc ctcagttcca cccgttgaag caccaatcgc aatcagtttt
     9661 tcagaactca acaacggccc tgccttcagc gttgtcggtg ccgacaatgg cttatgtgct
     9721 gcaaggctcg cctttgctgc tgtacgcacc ttttcagcaa tcatttcgct atacgccagc
     9781 attccttcgc gaatacccag ttgcggtttg gtgacaaaat ctatcgcccc cagctccagc
     9841 gcgcgcagcg tgacttctga ccctttgccg gtcaaggaag aaaccatcac aacgggcatt
     9901 ggacgcaaac gcattaattt ttcgaggaaa tccagtccgt ccatccgcgg catttcaaca
     9961 tccagcgtca gcacatcggg attgaatttc ttaatcaagt cacgcgcgac cagcggatca
    10021 ggcgcggtcg ccaccatttc catgtcgcta tggctgttga tgatttctgt catgatctgg
    10081 cgcatcagtg ccgaatcatc gacagataac accctgattt tgctcatcgt taatccttac
    10141 ttagcgcata caccgtctga ccacgcagcg tgaagcggcg ctcaaggtgg ctaaagtttt
    10201 cagagtgacc cgcaaacagc aatccgtcgg gtttaaggag cggaacaaag cggcgcaaaa
    10261 tctcctgctg ggtattttga tcgaagtaga tcatgacgtt acgacagaag atcgcatcaa
    10321 acggccccgg cacggtgtac tgtttcgcca gtagattcag cggggcaaaa tcaacatagt
    10381 tcgccagctc ctgacgcacg cgtaccagcc cttcatgcgg tcccgtccct cgcatgaaat
    10441 accgttgcag ttgctgcggc gtcaggtttt tcaactcttc atggcgataa ataccgcttc
    10501 tggctttttc cagcacttcg gtgtcgatat cactggcaaa cactttccag cgtccgggcg
    10561 cggtgcccaa tgtgtcagcc agcgtcatcg caatgctgta cggctcttcg ccggtcgaag
    10621 ccgccgcgct ccatacgcga tactcgccag aacggcgacg tgcgtgatcc gcgagcagag
    10681 ggaaatggtg tgcctcacgg aaaaatgccg tcagattcgt ggtcagcgaa ttgataaacg
    10741 cctgccactc accgctgtgc tgattagatt ccagcaagtt cagataatga ccgaaatccg
    10801 tcagtcccag cgaacgcaaa cgacgaacca gtcggttgta aaccatgtcg cgtttatggt
    10861 cagccagaac gatcccggct cgttgataga tcaattgact tatccgccga aaatgcgcgt
    10921 cggaaagcgc caggcgctcg gtcatctgta acaataaaga cgtttgccca cagggcagag
    10981 atgaagtcat agcgccttct caatcacttc aggataccac tggcgcaatt tgtaactgcg
    11041 ccgactcatg tcgtgctact tcatgttctt caagggtaaa taccgccaca cgcgacgaaa
    11101 gatggtcggc ctgattcgcc agttgttccg ttgccaccgc cgcttcttct accagcgagg
    11161 cgttctgctg agtcacctga tccatctggc tgacagcctg tgcaacctgc tcaatccccc
    11221 gctgttgttc ttccgacgca gaggcaattt ctcccataat gtcgttcacg cgggtcaccg
    11281 aactgacaat atcgatcatg gtcgcggcgg cgttattcac cagtttcgaa ccctgctgga
    11341 cacgattgac tgactcttcg atcagccctt tgatctcttt tgctgcctgc gcgctacggc
    11401 tggcaagatt gcgcacttca cctgccacta ccgcaaaacc acgcccctgc tctccggcgc
    11461 gagccgcttc cactgccgca ttcagggcca gaatattggt ctggaaagca attccgtcga
    11521 taacgctgat aatgtcgcca attttttgcg agctggtggc gatctcctgc atggtgtgag
    11581 tcatggtact gacctgaaca ccgcccgcct gcgccgttgt cgcggcattt tttgccagtt
    11641 ccgacgcctg tcgtgcgtta tcggcgtttt gccctaccgt ggcggttaat tgctccatac
    11701 tggcggccgt ttgtgccagc gatgccgcct gctgttcggt acgacttgag agatcgttat
    11761 tgcctgcgac aatctccgcg ataccaatgt gcatctcctg gcttccctta cgcacatcac
    11821 ttaccgtccc acgcaaagcc tgctgcatgg tcttcagact ggcaaaaatg gcggtgatct
    11881 cattacgacc atataccgca atcggacgcg ccagattacc cgcagcaatg ctgtcaaaat
    11941 ggctaccgat aatggccagt ggttgaacaa tcatcttgcg cgtccaccac agcgcactgc
    12001 tgatgtagat cgccgcaaca ataatcatgc tgataaacac cagcgccgaa atctgatagt
    12061 tacgctggct ttgcgcactg gcggcttcca gcacatggtt gatctccagc tgccaggcct
    12121 caaagttaac gtcaaacgca ttctgtgatt cctgcaccgg cgcagtgagg aaatccgaaa
    12181 gttgattact ttcaagccag gtcgcctgat gttcgagatc gttgtgccag cgggcaaaac
    12241 ttttctccgt ctctttttgc aacgccctga cgtgctcgtt gcccgcagtc atcgccataa
    12301 aacttttaaa cagcgtggtg gattgcgtca gactggcgcg cgccgtcgtc atcaacgttt
    12361 taatgtcatc cgccggatag ctaagcgccg tcagagtgcc cgctttgttc agcgcggtac
    12421 tggcctgtaa cattaccgcc cgagtttgcg ctaatgccgc acgttgctga ttgctctgct
    12481 cgacctgatt cagtcgttgc aaatcgtcgc gaaatgccca aaaagacatg ccgttactgc
    12541 caatctgcaa gatcccgcag agaatcaaaa ttaaaaacag cgtggtcgaa attcgaatac
    12601 gattaaacat caacgctccc catcaggcgg caatgaccgc gttagtaaat actcgtcaaa
    12661 atgtttccca gtttggatct tgttcagcaa ttcgcagtcg tggctgtgcc ggtggttgct
    12721 cactggcagg acgggatggt gtttgcggtt tattggtgag tgggctggct gccagacgga
    12781 acgcggaaac cgcttgcgtt aaacgactcg cctgttcttc cagcgcagcg gcggcggcag
    12841 ctgattcctg caccagcgat gcgttctgtt gcgtgacgcg atccatttcc gaaaccgcca
    12901 atgcgacttg atcgatgcca cggctctgtt catccgatgc cgatgcaatc tcgcccataa
    12961 tgtcagtcac gcgagtgaca gcattgacga tattgttcat tgtttccccg gcgctttcga
    13021 ccagcaccga accggtatca acgcgtgaga cggagtcttc aatgagggct ttgatctctt
    13081 ttgccgcctg ggcgctacga ctggcaagat tacgcacttc acccgccacc acggcaaaac
    13141 cacggccctg ttcacccgca cgcgcggctt caaccgcggc attcagcgcg aggatattag
    13201 tctggaaggc aataccgtcg ataacgctga taatgtcggc aattttcttc gaactatcgg
    13261 cgatctcatg catcgttttc actacgccat ccaccacttt gccgccgtgc tgggcggtgt
    13321 cggaggcact ttgcgccagt tgcgaggcct ggcgggcgtt atcggcgttt tgcttcactg
    13381 tcgcggtgag ctgctccatg ctggcggcag tttcttccag cgcggatgcc tgctgttcag
    13441 tacgggagga aagatcggtg ttgcccgccg caatttcacg ggtaccggca tagatggcat
    13501 ctgaaccttc gcggacatga gtgacggtgt cagtcaaaga gcgttgcata tgtgaaacgc
    13561 tctgcgccag gtcgcccatt tcactgcgcc cgtcaatggt cagggtattc gccaggttac
    13621 caccggcgat ttcgcgaatg tgagcaataa tttttgccag cggagtaagc aacatacggc
    13681 gaatgccgta ccacgccacc agcagaatca ataccaccac cagcgcgata accgccagtt
    13741 gccactgggc aaatcggtaa tcatctgcgt tgtcagtgac gatatcgcga tacagttttt
    13801 cactgctgag ggcgtactga gcaaacgctt cgcccattgc attttgcatt ccctgggttg
    13861 gctgagcgaa ataagctcca gtattgccat aatctagata atcaatcagt tcagttaacg
    13921 ctgtgtaata gtttttatat ttttcatcaa tattacgact ggtagcgacc atttcaggta
    13981 acggtgccat gcttttgaat tttttataat gcgtcgctgc ctgcgccaat gttttcctgg
    14041 cgctatcgag caattcaact ttggcgttac tttgttgatt ggaggaatcc atcatcatcc
    14101 gtaccgctga acgactcagg ttaatgcgcg tttgcagcat taaatcccag gttgacgtca
    14161 gctcgccctg ctgttcccgt aattgattgg aaaccacaaa gctcttctgg ctatggtgaa
    14221 gggaagaaaa aaacagactg ccggaaataa gctgtaacag tgcgaatacc cccagcacca
    14281 ttaccaacag cgtgactacg cggatacggt taatcataag gcaccttcct gaaaacaagt
    14341 tgatctcgtt atcggcaagg aggggggaaa ctttattgct gatgccaccc gccgcgaaat
    14401 tgaaataaaa aacccgatgc gcagatcatc gggttcattt caattgagga aatcgggaga
    14461 attacgccac ttctgacgcc gcgctatcta acagcgccat ctcttcgctg ttcagcagtt
    14521 tttcgatgtt caccagaatc aacatccggt cgcccagtgc gcccagtcca gtgagatatt
    14581 ctgttgaaag cgtcacggca aattccggtg ccggacgaat ttgctccgcc gtcaatgaaa
    14641 gcacgtctga gacgccgtca accacgatgc cgaccacccg ctgtccgaga ttcaggacga
    14701 taactaccgt gttgtcgtta tagtccacat ccacctggct gaacttaatt cgtaagtcaa
    14761 caatcggcac aataaccccg cgcagattcg tgacgccttt gataaacgct ggcgtgttcg
    14821 caatccgtgt tacctgatcg tagccacgga tctcctgcac tttcaggata tcaataccgt
    14881 actcttcatc accaagggta aataccagaa attcctggcc tgacggctcg ctggccagct
    14941 ttgttacatt cgtcataccg gtcatattgt taccttttta ctcattcagg cggcggtgtt
    15001 cgccatacgt tgttcgcggt ttatcgcctg caaggcggag acatcaacaa tcagtgccac
    15061 gctgccgtcg ccaagaatgg tcgcagcaga aatgccgggg actttgcgat agttactttc
    15121 aaggttttta accacaacct ggtgctgacc aattaattga tccaccagca aggcgtagcg
    15181 gcgaccgcca ctttgtaaga tcaccacaat tccctgggtg gcttcggttt tcgcgcccgc
    15241 gacgttgaac actttccaca gttcgacgat gggcagatat tcaccccgca cttccagcac
    15301 ccgctcgccg ccggccagtg gatgcagatc ggcttcacgg ggttgcagtg attccataac
    15361 agcattcagc ggcagaatga aaacttcatc cgcaacgcgt acggacatgc cgtcgaggat
    15421 ggccagcgtc agcggcagta aaatgcggat cgtagtgcca gtaccctgct tcgactggat
    15481 ttcgacatga ccgcccatct cctggatatt acgtttaacg acgtccatgc cgacgccgcg
    15541 cccggagacg tcggtgacct gctctgccgt ggagaagcct ggtgcaaata tcagcatcgc
    15601 gacttcgtcg tcgctcatgt tttcgctgac agtcaaacct tgcgaggccg cttttgccag
    15661 aattcgctca cggtttagcc ccgccccatc gtcggtcact tcaatgcaaa tgttgccgcc
    15721 ctgatgttcg gcagacagaa ttaaatttcc gacgctgttt ttacctgcgg cgagccgttt
    15781 ttctggcagt tcaataccgt gatcgaggct attgcgtacc aggtgggtca gcgggtcgat
    15841 aatgcgttct atcaggctct tgtcgagctc ggtggaactg cccaccagcg tcagttctac
    15901 ctgcttgccg agttttcccg ccagatcacg caccagccgg ggatagcgac taaagacata
    15961 ttccatcggc atcatgcgaa tcgacatcac tgattcctgc aaatcacggg cgttacgttg
    16021 taactgcccc atgctggtaa tcaaatcacc atgattaacc gggtccagtt cgctggaacg
    16081 ctgggcaagc atggactggg tgataaccag ctcgccgacg aggttaatta attgatcaac
    16141 cttttctacc gctacacgga tgctggtgga ttcactgctg cgcgtcgttt tttcccgctc
    16201 cacgcgaccg gttggcgctt gttcggctgc cagtttaagc actggtgggg tggatatttt
    16261 tggcgagact tctactgttt caaaggtaat ctgatcggct tcaatcacaa aacagagtac
    16321 cgctgtgatg tcatcttcgg cgatatcgcc cggtaatatt gccgagagag aatccgcccc
    16381 tttcaccacg tcagttaacg ttgtcagatg ccccagctct tcttccagca ggtcgacttc
    16441 gccggccttc aggcgcgaaa ggataattcg tcgcggcgac tgactgcgac tctgctcatc
    16501 ttgcggttca cttttggcaa ccacacttaa tcgggtcact gcggatggcg tttcgccttt
    16561 cgcttctaat gccagttgac gcaaggcctg gcagatataa tcgaagctgg cggcatccgg
    16621 ctcttgcgac tgtttataag cgtcgagctg ttcttgcatg atgtccttcg tttccaaaaa
    16681 cagattgata atgtcggtgt tgagttgcat ctcacctcgt ctggcttcat cgagcaggtt
    16741 ttccatcaga tgcgtggttt cctgcaaaac gctgaagcca aaagttcctg cccctccttt
    16801 gatcgagtgg gcagcccgaa agatggcatt caattgttcg gcatctggcg cttccggctg
    16861 caaaaccagc agatgctgct ccatatcagc caacagttcg tccgcttcat caaaaaatgt
    16921 ctgataaaaa tcgcttatat ccatgctcac gctgtcacct cggttcggct gatggcattg
    16981 tgggaacact gacctgtggt gcaacctcag gtttttccag ggcgcttact ggctcattct
    17041 ggctttcggc gttttcatgc aaaatggcct gttcggcttg tttgttcagt accagcaggc
    17101 tgatgcgacg gttgacggca tcatcaggtc cgcgatcgct taagcgcatc gttgccgcca
    17161 tgccgacgac acgtaacact ttgccgctat ccaaccctcc gaccatcagt tcgcggcggg
    17221 atgcattggc ccgatcggca gaaagctccc agttgctata tcctttctca ccgctggcgt
    17281 aggggaaatc atcggtatga cctgaaaggc taatacggtt gggaataccg ttcagtacag
    17341 gcgcaatggc acgcagaatg tcgcgcatat agggttcgac atcggcactg ccggttctaa
    17401 acatcgggcg attctggcta tcgatgatct gaatacgtag accttcctgg accagatcga
    17461 ttttgagatg gggacgtaac gcccgcagtt tcggatcgga ctctatcaac tggtcgagat
    17521 caccccgcaa tttccgcaat cgactttgct ccatgcgttt tttcagctct tcgatgttcg
    17581 gctgcttatt cacttccccc tggctttggg tgtaatcatc accaccgccg ggaattgggc
    17641 tttcactatt agaaatgcga tcgccgcccg taaccgcagt cgccagtgga gtccggaagt
    17701 actccgcaat ctgaatcagc tcttttgggc tggagatgga gatcagccac atcaccagaa
    17761 aaaaggccat catcgcagtc ataaagtcgg cataagcaat cttccacgat ccatgtgctg
    17821 ccccgtggct tttggctttg cgtcgtttga cgacaataat cggatgcgct tgattcttca
    17881 tgcttcctcg gttgtcgtct gttgttgcgg atttttcacc gcacgcacat gctcttccag
    17941 ttcaataaac gacggacgtt cgctggaata gagcgttttg cgaccaaact caacggcgat
    18001 aggcggtgcg taaccgttca gattagaaag cagagtgact ttgacgcact gcatcatttt
    18061 gctggtttcg gcgcttttct gacgtaaaac actcgctaat ggggaaataa atccgtaagc
    18121 caataaaatg ccgaggaaag tccccaccat cgcatgtgcg ataagcgcac ccagctcggc
    18181 ggcaggacga tcggctgaac ctaacgcgtg aacgaccccc attacagccg cgacaatacc
    18241 aaacgccgga agtgagtccc cgaccagcgc cagactgttc gccgggactt ctgcctcgct
    18301 ttcgtgcgtc tcaatctctt catccatcag ggcttcgatt tcgaaggtgt tcatatgacc
    18361 gctgataatc agacgcagat aatcgacgat aaaatcaagc atgacgctat ccgcgaggat
    18421 gcgtgggtag ctggcgaaga tctcgctctc acggggattt tcaatatcac gttccagcga
    18481 aaacatcccc atctgccgcg atttcgccat caaccgataa agcagagcca gcagatccat
    18541 atacattgct ttggtgtatt tggagcgacg aaacagcaac ggcagcgcct tcagcgtgcc
    18601 tttaatcgct ttgccattat tgccgacgat aaacgaccca atccctgcac cggcaataat
    18661 caccagttca gcgggttgat agagtgctcc aaggcttcca ccggtcatca aataaccgcc
    18721 gaaaactgta ccgagaacaa ccaggtaacc taataagata agcacgacat catccttcca
    18781 ctgttgacca tgacaggatg ttcagtcgtc aggcgttaac gcgcgattgg ggcaaaaaaa
    18841 agcagcggta cgtcgttacc gctgctggaa tgttgcgcct caccgtatca gttaaacagc
    18901 ctgtactctc tgttcatcca gcagttgtgg gataatatcg gcaggattct gggaaagttt
    18961 acgtcttttt actgcccggg atggcggttg acataagctg caggcaaagc tgccaacagg
    19021 ctggtgagcg tgggtaataa aattgccgcc gcagcagttg cagctggaaa gttgcagtaa
    19081 tccactttca acaaaccgca ccaatgtcca ggcacgggtt aatgccagca gtggtccttc
    19141 ttctgcttgt gggcactgtt caaggtataa acggtaggct ttgatcaccg catcgacgcc
    19201 attacacaaa ccggttttca gtaaaaactg ccatgcatta cagaacatcg aagcatgaac
    19261 gttttgttcc caggtcataa accagtcggt tgagaatggc agcatgcctt tcggcggtgg
    19321 gcttccgcgc agttctttat aaagttttat caggcgtccg cgacttaact gtgtttcgct
    19381 ttccagcatc tgcaaacgag ctcccagggt gatcaattcc attgccagct gaatatcccg
    19441 cgtttcctga acaatgcttt tttcactcat gatcaggccc ttttcttgcg cagcgcttct
    19501 tcaggctgat taacatcatt cagcaagcgt gttgagagca tgatgccggt atgaatttgc
    19561 tggagatcgt caacgcggga atcttgcgtc aactgagtaa tcgtctggtg gctgtcaaaa
    19621 cggaagtgac aaaccagttg attggtttct gccagcttaa ccatttgcgg aagagtcagt
    19681 gccgctaacg ttgtcgccat ttcttcattt atgccgagac gaaacatagc ggacgctttg
    19741 tcctgaacaa tcaaacgctg tgcaagtagt aaatatgaca agttgatgtc ataaatgtgt
    19801 ttcagcaact cggaggtatg cattattccc acccagaata accaacttta tttttatgcg
    19861 gtttcaccgc accccgtgat gtcgccggga agccccggta aaaaataatt agcattagaa
    19921 tagttgcgat aagctgcaat aagcagaacc acctttttgg tttaatatgt ccttacaaat
    19981 agaaatgggt ctttacactt atctaagatt tttcctaaat cgacgcaact gtactcgtca
    20041 ctacacgcac atacaacgga ggggggctgc gattttcaat aatgtgtgat gcagatcaca
    20101 caaaacactc aattacttaa cataaatgta taagtcatac ttttgttttg ggtgtatttc
    20161 aatctgttaa aaagtttttc gctacgctag caagcaaaaa tgaaacagga ataatcgaaa
    20221 tgggatgttg cgcacagtta aaataactca ccgtaaataa tcatctgcta taaataatca
    20281 ctttcatgca ataccagata agctattttt aaacagacac ttaccgcaca acaaactaat
    20341 taaatacaaa gaaaggcaac aatcaactta atgttaatga aatgttttac atatattaac
    20401 cacttgttta atgcggagaa tcagtacact attttcttat aattacattt gaaattatat
    20461 gatcaccggg agtgattgat taattgtatt tactaataat cagactaata tttacctgtt
    20521 tgaccgagtt gggattgcgt cgtttctcca ttaggagtaa agctttaatg tcacctgaag
    20581 ttcacagaat aaggaacagg ttatgagcta tagcaatatt cttgtcgctg ttgcggttac
    20641 accggaaagt cagcaactgc tggcaaaagc ggtatctatc gccaggccag taaagggaca
    20701 catcagttta attactctcg cttccgaccc ggaaatgtac aatcaattag ccgcgccgat
    20761 gcttgaagat ttacgcagcg tgatgcaaga agaaacgcaa agctttcttg ataagttaat
    20821 tcaggatgcg gggtatcccg ttgacaagac gtttattgcc tacggtgagt taagcgaaca
    20881 tattctggaa gtatgtcaca aacatcattt cgatttggtg atttgcggta atcacaatca
    20941 cagtttcttt tcgcgagcat cctgctcggc gaaaagagtg attgcctcaa gtgaggtcga
    21001 tgtgctgtta gttccactta cgggtgatta accgctccta cgcaagcttt ggaaaggtag
    21061 caactttatc gcgctgctgg ctttccgcgc ttcgcggaac tatctgcttt aggtcgctaa
    21121 tgaagcactc ctgccagtgg ttaatatcgt ttttcacgat aacgtccagc atttctgcat
    21181 gacgggaaat acgttccgcc agcgacatag tcaatgcacg atccagcgca gctgcaactt
    21241 cgtcacgatc gtaggggtta acaattaacg ccgacgttaa ctcgtttgcc gctcccgcaa
    21301 attgcgaaag aacaagaacg cccgggttgg ctgggtcctg agcagcaaca tactcttttg
    21361 ctaccaggtt catcccgtca cgcagtggcg tcactaagcc cacgtcagag tagcggaata
    21421 ttttcatcag taatttacgg tcaaaatgct gattcaaata ataaagcggc gtccagccta
    21481 attgcccgta tttaccatta attcgtccag cttcattttc gagctgatga cgaatatcct
    21541 gataggcttg cacatcacca cgcgacgttg gtgcaatctg ggtataacga attttaccat
    21601 gatgctgcgg atatttttcc agcaacgctt cataggcgag aaaacgctct ggcaaacctt
    21661 tggaataatc cagccgttcg acagaaaaga tattttgtac gtttttcagt tccgctttaa
    21721 gttgcgccag ttttggcggc agtggcccgg cagcctgttt ggctatttct ttcggttcaa
    21781 tgccgatcgg gtagacttct gttcgaaatg ctttgcccca ggctgtatgg ctttttgcgc
    21841 tacgtgtcgt gacgcgggtc aggttagaaa gacaatccag gaacgccaga cgatcgtttt
    21901 ctgtctggaa acccagcaaa tcataatcac aaagctgttc aagcaaggtg tcatatgtcg
    21961 gcagcgcgtt gaagatttcc ggtgtcggga aaggaatatg cagaaagaaa ccaatgcgat
    22021 tattcactcc ccgtttgcgt aattcatgcg caaatggcaa caggtgataa tcgtggatcc
    22081 agataatgtc atcgtcttgc aacagcggca gtaatttatc tgccagcaac gcatttacgc
    22141 gtagatagcc gtcccaggca ggacgctgaa attgcaccag atcgagccga taatgaaaag
    22201 cgggccagag aacggcattg gagaattggt tgtagtattc gtcaaggtcc tgttcgctga
    22261 ggttaaaaga ggcccacgta atgttacctt ttttcacctt ttttagcggc tgatcctcat
    22321 tccctgtttc accactccag ccaaaccaca gtccgcctgc ggctttcagt gcccccagta
    22381 tgccaacggc aaggccaccg gcactggcgg cgtgctcgtc tggtggtgca atccggttag
    22441 atactacgac taaacgactc atagtcatca ctcctgttat tttctctttt ttgttgtaat
    22501 gcggtggtta tcatttcaag ccagctccag acatccggca cacccgccag tcgccatgat
    22561 gcctgagttg cacctgtgcc aatttttact gacattccgc ccagtcggtt aacgactgcg
    22621 aagccagatt catcggttaa atcatcgccc agaaatacgg gcgttcgccc gataaaggga
    22681 gcttcctgca taaaagctgc aattgcctca cctttactgg tacctctcgg tttgatctcg
    22741 acaacacact ttccctgctg taacgccatt tgtggccaga tctgagtaat acgttgcgct
    22801 aatgtcatta atgcgtcttc atgctgcgga gcctgacgat aatgcagcgc aaaagccatc
    22861 cctttcgcct ccagctccgc gccgggatac tgagcgatga ctgtatgcag ttgcacgcta
    22921 atatcacgcg caatcgcatc cggcagatga acgatatgtg ttttaccatt gatgtcacgg
    22981 cgctccgccc catgcacgcc cgctaacggg aagcgataag gttttgccag tgcgtcaagc
    23041 tccaccattg agcgccctga tatcaatgcc aatgcaccat cacttgcggt tgccagtagc
    23101 tgtagtcctt gcagaatatt gtcaggcacg acgacctgat cgggatgcgg tttgatttcc
    23161 gccagcgttc catcaagatc aaaaaaccag gcatatttcg cggatagttc aggggtttcg
    23221 gttaacggtt ctgtcacccg gctctcctcc ttctcttttc ttattgtttt gcgcagaacg
    23281 cgctttacag acatgtaagt atagacagtg tgacgggggt cgccatttgc aatgaagaac
    23341 agccaaccgt tgtggcggct ggctgtggtg ggaaaaaacg ttaaattgtt gtggaaaaaa
    23401 gcatcagaca gtgcgtttcg ctttttgctt gtaacggtcg aagatcaccg ctgccagcag
    23461 gattaagccg cgaaccacgt actgcgcgaa aggagaaata ttaagcaggt tcatggcgtt
    23521 ttccacggtg cctaaaatta agatacccgc caccacatat gagatttttc cgatgccacc
    23581 tttcagagaa acgccaccta aaacgcaggc ggagataaca ataagctcat aaccaatcga
    23641 cgtcattggc tgcccactag tcatacgtga agccagaata attccggcta tcgcagatac
    23701 caggccagag agaacaaaga taataatttt ggtgcgaaca accggtacac ccgccagacg
    23761 cgcagcctct tcgttcccgc caatcgccag ggtgttacga ccaaaggtgg ttttattcag
    23821 caacaaacca aagataatca gacacgcgac ggtgagccag attggcgcag gcagaccgaa
    23881 ccagttagcg taaccaaggg caaagaagct ttcatcttcg ataccgaccg ctttaccgtc
    23941 tgaaatgatg tacgccagac ctcgaacaat ctgcatcgtt gccagtgttg tgatcagagc
    24001 atttattttc agtttggcga taacaaagcc attgaccagg ccacagagaa tgccaagcag
    24061 caaccccgct gccacgccaa tccacaggct ttcagtcagg ttgataacca ccgccgtggt
    24121 gacacccgca caggcaatta cggaggcgac agaaaggtca aagtcaccgg aagcaaggca
    24181 gaacaacatg ccacacgcca ccatccccga catggaaatt gccaggccca accctttcat
    24241 attaatgaag gtggcaaaat ttgggacaaa aatggcacag gcgataaaga gcaccgcaaa
    24301 caccaccagc atgccgtact gatcccagat acgcccgaag ctgaatgacg acttaggtgc
    24361 gccagacccc gatgtagaaa cagaagacat catactctcc ttactcaggc aactgcctgg
    24421 ctgactttag gcatcgcaag gctcagtgcc tgacgctcat ctgcctgctc gtgtaacaat
    24481 tcaccggcga tttcaccttc ccgcatcacc acaatccggt cggcaacgcc gaggacttca
    24541 ggtaagtcgc tggaggcaaa cagcaccgcc acaccctgcg ccgccagcgc ataaatcacg
    24601 ttgtaaattt catgcttagc accaacatca atgccgcgcg taggttcatc cagcaaaatg
    24661 accttcatct cttccgataa ccagcggccc agaatggctt tttgctgatt tccgcctgag
    24721 agattcatga tcagttgctc agcgcccggc gttttgatgt tgagcgaacg aatgtggtga
    24781 tcggcattgt tttcttccca accgttgttg attacacaac cgccgagcac atgtttacgt
    24841 ctggcactga tgttgatatt gtcgcgaacg gagtgcacgg gaataatgcc ttccgctttg
    24901 cgatcttccg ggcagggcat catgcctgcg gcaatggcgt ggctcggttt acgaatatcg
    24961 atcggctgtt ggtcgatata aacctgaccg gcggtgattt gcgtcccgcc aaacaagcct
    25021 ttcattaatt cgctacgccc cgctcctacc agaccaaaca gcccaacaat ttcaccactg
    25081 cgaaccgcca gacttattgg cgtacgcacg cctggtgctt tcacagcatc aagacgcagg
    25141 cgctcctcgc cataactacg aagttgccag ccgtagatat cgccaatgtc gcgcccgacc
    25201 atcgcctgca ccagcgcgtc gtggtcaacc tgctgcatat cggtaaaggt tttgacataa
    25261 cgtccatctt taaagacagt gatggcatcg ctgagggcaa atatttcttc catacggtga
    25321 gaaacgtata agatcacccg cccctctttt cgcagttcac gaataacgcg gaaaagattg
    25381 tcgatttcgc gggcagaaag ggagctggtt ggctcatcaa aggcgataat tttagcgtta
    25441 cgcgccagcg ccttggcgat ttcaaccatc tgccactgac caatggagag atatttcagc
    25501 ggcgtgtccg ggtcaatatc cataccaaga tgtttaagtt gtaaacccgc ctcataattc
    25561 agcaatgagc gattcacaat gccgccttta tgcggcagct ggccgagata gatgttttcc
    25621 gcgacggtca tttccggcac gagatgcagt tcctggtaaa taatcgccac acccgcatta
    25681 agtgctgcgg tcgtgtcgga aaaggacatt tcctgcccat taatcactac agaacccgtg
    25741 gttggcgcat agttgccgct gaggattttt aagagagttg attttcctgc gccattttca
    25801 cccatcaacg catgaacctg accggcatag cagtcaaaac taatatccgt cagcgcctta
    25861 acgccgggaa acgttttacc gatgccgcga aatgagagat acggggtaga ctgttgcata
    25921 acgtctccgt gaatcactgg tcgttcacac cccctctgca tcatgcagag gggaattttt
    25981 ccggcaaatt acttaccgcc taaacctttt ttctccagtt cttctttaaa gttgtcacgc
    26041 gtgatcagta ccacgtcggt aacttcggta aattttggcg gttcaacgtc ttttgctacc
    26101 cagttgtaaa gcatttcgct ggatttatag ccatgtacgt ccgggcttgg cagcagggaa
    26161 ccgtagaagc cggttgcctg tgctttagac agttcgctca ccgcatccac accgttaatg
    26221 ccaatgccga tgatatcggc cgctttaaag ccctgacctt ccgtcgcgcg tacgccgccc
    26281 agcacggtgc tgtcgttcat accgacgatc agccaatgtt taacttccgg atgttgaacc
    26341 agcattgagt tggcagcgtc aaatgccccc gggatgtcgt tagatttggt aggtacctga
    26401 taaatttgtt tttccgggaa tccggccgct ttcagcgcat ccatagatcc cgtagtacgg
    26461 cggcgggcgg tatccagttc gttggcggta atcgccatca ccgcgctttc tttgacatcc
    26521 cagccacgtt tctgcatctc tttatacagt tcctggccct gacgttcgcc aattttagtc
    26581 gccgccatca tcaccagcgg aacggtatcc attggcttac ctttggcgtt aacaaactgg
    26641 tcatccacgg caatgacttt catatcgtag ccacgcgctt tcgcgacgat ggcagagccg
    26701 agtttggggt ccggagtaca aataacgaaa ccttttgcgc cactggcagc caggctgtcg
    26761 atcgcgttca atgttttttc gccatccggc acggcaatct taataacctc aaaccctaaa
    26821 tccttcccgg ctttatcggc aaacttccat tcggtctgga accacggctc ttccggttgc
    26881 ttcaccagaa aaccgagctt caggttctcc gccatagcgg attgtgacat aacggctgcc
    26941 agaccaatgg ctgccagggc tttagtaaat ttgtgcatgg ttctctccag ctttagtgtc
    27001 gttttgtgta gggcaaaaac gaatgacatt cgttaaatta atcggaaaac aaagcattac
    27061 cttttaacta aaagataagt gaccgtgttg acatagtttt agcgagaaat taattctcca
    27121 taggagagca atatcacatc gcagaattac agtgagaacg tgcataaatt tagcgggaaa
    27181 agacataagg gaaagccaat ttgtcagaca aattgtcgaa tgcacagcag attaatccat
    27241 aagattagcc tggaaatcct tgttgtcttt ggtacccatg cgggatgtct tctttttaac
    27301 cagtcaatag gccgcattac ctggcgttga gtttttgaaa tggtgtaata accgcaactc
    27361 aaagatgtgg aaaatgcacg tcattcattt cgtcattaat tatcactgtg ctcattaatt
    27421 aacagaacac gtataatgag agccatctcg caaaaatgaa aaaacgtttt ataaaatcat
    27481 cacttcatca tgaattcaaa ttcattgatt aatatcaaca agatacaaaa agcactatca
    27541 ttaaaattca ttgcagttac attgatttca tcaatgaaat gtaaaaatat ataaacttga
    27601 tgatttaagc attttcttat acccgttcag acgttattct tatttcagat catcgtcaga
    27661 attgactcca cgatcacatt tcggaccggc agaaaggaat tattctgcaa acagtaatta
    27721 tggtgttttg atttatcttg cacctctcca cttctggata taaggatatt aggtatggca
    27781 accgctggaa tgcttctcaa actcaactct caaatgaacc gcgagtttta cgcatccaat
    27841 ctctaccttc acctgagtaa ctggtgttct gaacagagtc tgaacggcac cgccactttc
    27901 cttcgcgccc aggcacagag taatgtgacc caaatgatgc gcatgtttaa ctttatgaag
    27961 agtgtcggcg ctacccccat cgttaaagcc attgatgttc ccggtgaaaa actgaactct
    28021 ctggaagaac tgttccaaaa aacgatggaa gaatacgagc aacgttctag tacgttggca
    28081 cagttagccg atgaagcgaa agaactgaat gatgattcaa ccgtcaattt cctgcgcgat
    28141 ctggaaaaag aacagcagca tgatggtctg ttgctgcaaa ccattcttga tgaagtgcgc
    28201 agtgcgaaac ttgcgggtat gtgccctgtg cagaccgacc aacatgttct gaatgtcgtg
    28261 tcacaccagc tgcattgatc atcatcggcg ctaatgcatt gcgccgatga aggttttgag
    28321 aaaccgctgc ctcatctgtt tgaagcagcg gtttttttaa tgggattcac cctgtggggt
    28381 aaacttgagt tcaataagcg cgatggcttt ttggattgcc cgcatggtga ccgggtctgc
    28441 ggcggcggga tggttagtaa agtcgatatt cttcagctga ctggacattt tttcacgaac
    28501 ttcaacgggc gcgattacat cgagaacatc cagaatttgt ttgataacca actggcaagc
    28561 aaccacatca gaaaccagtt cctgatcggc attcagcggc tgggacatcg taaactcctg
    28621 atagcatttt gaaagccgtt atagtagcgg cttcacatct tcagcgatag tcacatccac
    28681 cgtcatcagg acacaaaaaa acctgccgga gcaggttttt tgttatcgga acatattgcc
    28741 tggcggtacg tctttgaacg tcttgcaata gttattgaac atacttttca ggattttgcg
    28801 cagtttcatc gcggcactcc gaccatttgt tatacaggtg ttattgtctt tgcgcttata
    28861 atatgacaac catcacaaaa atcaatcttt atatgataca aatcacataa ataccccttt
    28921 aatgttataa aaatgataat caaaaaacag cccccctatt tctgacacct acagatggca
    28981 agaaatagcg cctgccaggc gtcttttccg gccattgtcg cagcactgta acgcgtaaaa
    29041 tagtgctttc tcttactctt ctggctggac catgagactt ctgattctga ctctttcatt
    29101 aataacgctg gcggggtgta cggttactcg ccaagcccat gtgagcgaag ttgacgcggc
    29161 aacaggtatt gtacggttag tttatgacca ggcttttttg cagcatgccc ataccgatcg
    29221 ttatgtcagt cgcgggatcg ccgatcgcgc atgccagcag gaaggctata cccacgcggt
    29281 cccctttggt cagccagtag gcaactgtag cctttttgcg ggttccttat gtttgaatac
    29341 ggaattcact ttgtcatatc agtgccatca ttccgccttc cctgtctttc tctaaatatt
    29401 ttaatcagcg aggggatctt cgctgattaa agaaatagcg gtaatgcgtt ttattcccat
    29461 tcgtattttt aataattgaa gtttatattt taccttttgc aaataataaa ataacaaatt
    29521 atagtgacgc cacagcaaca aatataacct ttgtggagca ctatcatgct gaaaccagaa
    29581 atgattgaaa aacttaatga gcagatgaac ctggaactgt actcttcact gctttatcag
    29641 caaatgagcg cctggtgcag ctatcatacc ttcgaaggtg ctgccgcgtt cctgcgccgt
    29701 cacgcccagg aagagatgac gcatatgcag cgtctgtttg attacctgac tgataccggc
    29761 aatttaccgc gtattaatac cgttgaatct ccgtttgctg aatattcctc acttgatgaa
    29821 ttattccagg aaacctataa acacgaacaa ttaatcaccc agaaaattaa cgaactggct
    29881 catgctgcaa tgaccaatca ggactaccca acatttaatt tcctgcaatg gtatgtttct
    29941 gagcagcatg aagaagagaa actgttcaaa tcgattattg ataaattaag cctggcaggc
    30001 aaaagcggcg aaggtctgta ttttatcgac aaagaactct ctaccctcga cacacaaaac
    30061 taatgctctc cggcggcagg tttactgccg ccgtttcaaa tcagtgacgg caaatcttac
    30121 tctgatcggt cgaaaaaccg tctttcgaag gtataaattt gccttttgct gccagaaacg
    30181 ctaccagctc tcccgctgtc atcccttctg ccgagcaggt gtgaaaacgt gcctgttcac
    30241 caaaacgcgc tttaatcgca gcttccagac tggcatgcgt atattgctcg cctgattcaa
    30301 tcatcatatt taacacttca tgaccgtgaa tagagtccat cgtccctcct caaaaaaagc
    30361 ctagcgtagc gattgccgct tatgaagact ttgcgccagc gcaggactga atgcttttta
    30421 ttgtacattt atatttacac catatgtaac gtcggtttga cgaagcagcc gttatgcctt
    30481 aacctgcgcc gcagatatca ctcataaaga tcgtcaggac agaagaaagc gtgaaaaaca
    30541 gaaccctggg aagtgttttt atcgtggcgg gaaccacaat tggcgcaggc atgctggcaa
    30601 tgccgctggc tgcggccggt gttggtttta gcgttacgtt aatcttgttg attgggcttt
    30661 gggcgttgat gtgctacacg gcgctattac tgctggaggt gtaccagcat gttccggcag
    30721 ataccggtct gggcacgctg gcaaaacgct atctgggacg ctacggtcaa tggctgacgg
    30781 gcttcagtat gatgttctta atgtatgctc tgactgcggc atacatcagc ggtgccggtg
    30841 aattgttggc ctccagcatc agcgactgga caggtatttc aatgtcggca accgctggcg
    30901 tgctgttgtt cacttttgtt gccggtggcg tggtttgtgt cggaacttca ctggtcgatt
    30961 tatttaaccg ttttctgttc agcgccaaga ttatttttct ggtggtaatg ctggtactac
    31021 tgctgccgca tattcacaaa gtgaatcttt taaccctgcc gttgcaacag gggctggctc
    31081 tgtctgcaat cccggtgatt tttacctcat ttggttttca cggtagcgtg ccgagtattg
    31141 tcagctatat ggatggcaac gttcgtaagc tacgctgggt gtttataacc ggtagtgcga
    31201 tccccctggt ggcatatatt ttctggcagg tggcaacgct tggcagcatt gattcaacaa
    31261 cctttatggg attgctggct aatcatgccg gattaaacgg gctgttacag gcattacgcg
    31321 aaatggtggc ctctccgcat gttgagctgg cagtgcattt atttgctgat ttagccctcg
    31381 ccacgtcatt tctcggcgtt gcgttaggct tatttgatta tctggctgat ttatttcagc
    31441 gttcaaatac cgttggtgga cggttgcaaa ctggtgcaat tacctttctg ccgccgttgg
    31501 cgtttgcact gttttatcca cgaggatttg tgatggcgct gggttacgcc ggtgtggcgc
    31561 tggcggtact ggcattgatt atcccttcgc tgttgacctg gcaaagcaga aagcacaatc
    31621 ctcaggcggg ttaccgggtg aaaggtggtc gtccggcgct ggtggtggtg tttctctgtg
    31681 gtattgctgt gattggcgtg caatttttga ttgcggcagg gttgttacca gaagtggggt
    31741 gatcagatag cctcaaattc cttattgggt gccagaatta acgctgacac ccaatttgac
    31801 ctcttaatgc aggcagcact gcttaaattt cttaccacta ccgcacgggc aaggatcgtt
    31861 acgccccggt ttctcttctg ctttgatcgg ttgctgaaca gctttttcct gcggatgcgc
    31921 catccagtac gcatgtagat caagcgccgc cagtcgaatg gcatctacgc tctcttcaaa
    31981 cgcttctggc gacattttct ctacccgctc gaagttttcc tcagtaccgt gcagcgcaat
    32041 cgcctccagc gctggtttta acgaatcggg caacgttgac cagtcagaaa gtgccacgcc
    32101 ccgcatatag ccaaagcacc actcctcaac aatcgtcagc tcgctgccat caacttctcg
    32161 caagccgaat aacggctcaa actgctccgg gaattcgttc agacgctctg cggtatcggc
    32221 catatgttga aaagccagat tcataaagcg cgtcatctct ttctctgacg cccagcgcgg
    32281 cacatagtca gccccacccc acacggcaac cagccactgt tccggttcaa tctcttgcgg
    32341 agaactcaac accgccgtca ataaaccgtc cagctccgcc acatcaagga tggcgtggtc
    32401 agtgttgtat ttggtcagaa tatcgtccag ccattccaac tcactttcgt ttaacggtcc
    32461 cgttttcata cgcttttcct tgtggatctc aactcgccag cacctatctt acatgccggt
    32521 ccgtatcaga gatacttttt gagtggcttt gctggtgatt aaaaattaag gagggtgtaa
    32581 cgacaagttg caggcacaaa aaaac
//
LOCUS       DAZFQD010000042        32567 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.042, whole genome shotgun sequence.
ACCESSION   DAZFQD010000042 DAZFQD010000000
VERSION     DAZFQD010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32567
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.042"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(324..536)
                     /locus_tag="ACHKTW_002063"
                     /pseudo
     CDS             complement(324..536)
                     /locus_tag="ACHKTW_002063"
                     /inference="COORDINATES: protein motif:HMM:NF015639.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            complement(676..763)
                     /locus_tag="ACHKTW_002064"
     tRNA            complement(676..763)
                     /locus_tag="ACHKTW_002064"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(727..729),aa:Ser,seq:tga)
     gene            1190..2308
                     /gene="hyaA"
                     /locus_tag="ACHKTW_002065"
     CDS             1190..2308
                     /gene="hyaA"
                     /locus_tag="ACHKTW_002065"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HFO6463090.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            2305..4098
                     /gene="hyaB"
                     /locus_tag="ACHKTW_002066"
     CDS             2305..4098
                     /gene="hyaB"
                     /locus_tag="ACHKTW_002066"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HFO6463091.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHLQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFDKLMTNLKNGNLATASTEKWEPATWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            4117..4824
                     /gene="hyaC"
                     /locus_tag="ACHKTW_002067"
     CDS             4117..4824
                     /gene="hyaC"
                     /locus_tag="ACHKTW_002067"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HFO6463092.1"
                     /translation="MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMVFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWTGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMV
                     NGYRSHKFGKISNKERS"
     gene            4821..5408
                     /gene="hyaD"
                     /locus_tag="ACHKTW_002068"
     CDS             4821..5408
                     /gene="hyaD"
                     /locus_tag="ACHKTW_002068"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HFO6463093.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELARDQLPAAEQSALAQLAAWGIVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTLEEQG"
     gene            5405..5803
                     /gene="hyaE"
                     /locus_tag="ACHKTW_002069"
     CDS             5405..5803
                     /gene="hyaE"
                     /locus_tag="ACHKTW_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HFO6463094.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSESRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLREFPDYTWQVAIADLEQSEAIGDRFGVFRFPATLVFTGGN
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            5800..6657
                     /gene="hyaF"
                     /locus_tag="ACHKTW_002070"
     CDS             5800..6657
                     /gene="hyaF"
                     /locus_tag="ACHKTW_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HFO6463095.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQMSQRQPPLGEVLAVDLLNLHADDRHFINTLLGEGEVSVRIQQA
                     DDSESEIQEAIFCGLWRVRRRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPT"
     gene            6791..8335
                     /gene="appC"
                     /locus_tag="ACHKTW_002071"
     CDS             6791..8335
                     /gene="appC"
                     /locus_tag="ACHKTW_002071"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HFO6463096.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            8347..9483
                     /gene="appB"
                     /locus_tag="ACHKTW_002072"
     CDS             8347..9483
                     /gene="appB"
                     /locus_tag="ACHKTW_002072"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HFO6463097.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            9496..9588
                     /gene="cbdX"
                     /locus_tag="ACHKTW_002073"
     CDS             9496..9588
                     /gene="cbdX"
                     /locus_tag="ACHKTW_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HFO6463098.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            9668..10966
                     /gene="appA"
                     /locus_tag="ACHKTW_002074"
     CDS             9668..10966
                     /gene="appA"
                     /locus_tag="ACHKTW_002074"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HFO6463099.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPELKLESVVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKKGCPQS
                     GQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILSRAGGSIADFTGHRQTAFRELERVLNFPQSNLCLKREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIMAALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(11081..13261)
                     /gene="etk"
                     /locus_tag="ACHKTW_002075"
     CDS             complement(11081..13261)
                     /gene="etk"
                     /locus_tag="ACHKTW_002075"
                     /EC_number="2.7.10.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HFO6463100.1"
                     /translation="MTTKNMNTPPGSTQENEIDLLRLVGELWDHRKFIISVTALFTLI
                     AVAYSLLSTPIYQADTLVQVEQKQGNAILSGLSDMIPNSSPESAPEIQLLQSRMILGK
                     TIAELNLRDIVEQKYFPIVGRGWARLTKEKPGELAISWMHIPQLNGQDQQLTLTVGEN
                     GHYTLEGEEFTVNGMVGQRLEKDGVALTIADIKAKPGTQFVLSQRTELEAINALQETF
                     TVSERSKESGMLELTMTGDDPQLITRILNSIANNYLQQNIARQAAQDSQSLEFLQRQL
                     PEVRSELDQAEEKLNVYRQQRDSVDLNLEAKAVLEQIVNVDNQLNELTFREAEISQLY
                     KKDHPTYRALLEKRQTLEQERKRLNKRVSAMPSTQQEVLRLSRDVEAGRAVYLQLLNR
                     QQELSISKSSAIGNVRIIDPAVTQPQPVKPKKALNVVLGFILGLFISVGAVLARAMLR
                     RGVEAPEQLEEHGISVYATIPMSEWLDKRTRLRKKNLFSNQQRHRTKNIPFLAVDNPA
                     DSAVEAVRALRTSLHFAMMETENNILMITGATPDSGKTFVSSTLAAVIAQSDQKVLFI
                     DADLRRGYSHNLFTVSNEHGLSEYLAGKDELNKVIQHFGKGGFDVITRGQVPPNPSEL
                     LMRDRMRQLLEWANDHYDLVIVDTPPMLAVSDAAVVGRSVGTSLLVARFGLNTAKEVS
                     LSMQRLEQAGVNIKGAILNGVIKRASTAYSYGYNYYGYSYSEKE"
     gene            complement(13281..13727)
                     /gene="etp"
                     /locus_tag="ACHKTW_002076"
                     /gene_synonym="yccY"
     CDS             complement(13281..13727)
                     /gene="etp"
                     /locus_tag="ACHKTW_002076"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HFO6463101.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     VKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYGMLERASQEWAKRLSR"
     gene            complement(13715..14854)
                     /gene="gfcE"
                     /locus_tag="ACHKTW_002077"
     CDS             complement(13715..14854)
                     /gene="gfcE"
                     /locus_tag="ACHKTW_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HFO6463102.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            complement(14900..16996)
                     /gene="gfcD"
                     /locus_tag="ACHKTW_002078"
     CDS             complement(14900..16996)
                     /gene="gfcD"
                     /locus_tag="ACHKTW_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HFO6463103.1"
                     /translation="MKKNSYLLSCLAIAVSSACHAEVLTYPDPLGSSQSDFGGTGLLQ
                     MPNARIAPEGEFSVNYRDNDQYRFYSTSVALFPWLEGTIRYTDVRTRKYSQWEDFSGD
                     QSYKDKSFDFKLRLWEEGYWLPQVAFGKRDIAGTGLFDGEYLVASKQAGPFDFTLGMA
                     WGYAGNAGNITNPFCRVSDKYCHRAKSHDAGDISFSDIFRGPASIFGGIEYQTPWNPL
                     RLKLEYDGNNYQNDFAGKLPQASHFNVGAVYRAASWADLNLSYERGNTLMFGFTLRTN
                     FNDLRPALRDTPKPAYQPAPESEGLQYTTVANQLTALKYNAGFEAPEIQLRDKTLYMS
                     GQQYKYRDSREAVDRANRILVNNLPQGVEKISVTQKREHMAMVTTETDVASLRKQLAG
                     TAPGQSEQLQQQRVEAEDLSAFGRGYRIREDRFSYSFNPTLSQSLGGPEDFYMFQLGL
                     MSSARYWFTDHLLLDGGIFTNIYNNYDKFKSSLLPADSTLPRVRTHIRDYVRNDVYLN
                     NLQANYFADLGNGFYGQVYGGYLETMYAGVGSELLYRPLDASWALGVDVNYVKQRDWD
                     NMMRFTDYSTPTGFVTAYWNPPTLNGVLMKLSVGQYLAKDKGATIDVAKRFDSGVAVG
                     VWAAISNVSKDDYGEGGFSKGFYISIPFDLMTIGPNRNRAVVSWTPLTRDGGQMLSRK
                     YQLYPMTAEREVPVGQ"
     gene            complement(16996..17742)
                     /gene="gfcC"
                     /locus_tag="ACHKTW_002079"
     CDS             complement(16996..17742)
                     /gene="gfcC"
                     /locus_tag="ACHKTW_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HFO6463104.1"
                     /translation="MNKLQSYFIASVLYVMTPHAFAQGTVTIYLPGEQQTLSVGPMEN
                     VVQLVTQPQLRDRLWWPGALLTDSAAKAKALKDYQHVMAQLASWEAEADDDVAATIKS
                     VRQQLLNLNITGRLPVKLDPDFVRVDENSNPPLVGDYTLYTVQRPVTITLLGAVSGAG
                     QLPWQAGRSVTDYLQDHPRLAGADKNNVMVITPEGETVVAPVALWNKRHVEPPPGSQL
                     WLGFSAHVLPEKYADLNDQIVSVLTQRVPD"
     gene            complement(17739..18383)
                     /gene="gfcB"
                     /locus_tag="ACHKTW_002080"
     CDS             complement(17739..18383)
                     /gene="gfcB"
                     /locus_tag="ACHKTW_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein GfcB"
                     /protein_id="HFO6463105.1"
                     /translation="MRPLILSIFALFLAGCTHSQQSMVDTFRASLFDNQDITVADQQI
                     QALPYSTMYLRLNEGQRIFVVLGYIEQEQSKWLSQDNAMLVTHNGRLLKTVKLNNNLL
                     EVTNSGQDPLRNALAIKDGSRWTRDILWSEDNHFRSATLSSTFSFAGLETLNIAGRNV
                     LCNVWQEEVTSTRPEKQWQNTFWVDSATGQVRQSRQMLGAGVIPVEMTFLKPAP"
     gene            complement(18490..18795)
                     /gene="gfcA"
                     /locus_tag="ACHKTW_002081"
     CDS             complement(18490..18795)
                     /gene="gfcA"
                     /locus_tag="ACHKTW_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine-rich inner membrane protein GfcA"
                     /protein_id="HFO6463106.1"
                     /translation="MKHKLSAILMAFMLTTPAAFAAPEATNGTEATTGTTGTTTTTTG
                     ATTTATTTGGVAAGAVGTATVVGVATAVGVATLAVVAANDSGDGGSHNTSTTTSTTR"
     gene            complement(19237..19449)
                     /gene="cspH"
                     /locus_tag="ACHKTW_002082"
     CDS             complement(19237..19449)
                     /gene="cspH"
                     /locus_tag="ACHKTW_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HFO6463107.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
     gene            19735..19947
                     /gene="cspG"
                     /locus_tag="ACHKTW_002083"
     CDS             19735..19947
                     /gene="cspG"
                     /locus_tag="ACHKTW_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HFO6463108.1"
                     /translation="MSNKMTGLVKWFNADKGFGFITPDDGSKDVFVHFTAIQSNEFRT
                     LNENQKVEFSIEQGQRGPAAANVVTL"
     gene            19958..20146
                     /gene="ymcF"
                     /locus_tag="ACHKTW_002084"
     CDS             19958..20146
                     /gene="ymcF"
                     /locus_tag="ACHKTW_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFO6463109.1"
                     /translation="MTQHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVALQIRTDHAPLDFTK"
     gene            20121..20351
                     /gene="ymcE"
                     /locus_tag="ACHKTW_002085"
     CDS             20121..20351
                     /gene="ymcE"
                     /locus_tag="ACHKTW_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmcE"
                     /protein_id="HFO6463110.1"
                     /translation="MRRWISQNNIRLPRGAFFISALFFFNAVCIVSDNLLIIESFGEM
                     AYNISYLTRVPGTNTLLACCCLLRPEEVNSEY"
     gene            20341..20514
                     /gene="gnsA"
                     /locus_tag="ACHKTW_002086"
     CDS             20341..20514
                     /gene="gnsA"
                     /locus_tag="ACHKTW_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HFO6463111.1"
                     /translation="MNIEELKKQAETEIADFIAQKIAELNKNTGKEVSEIRFTAREKM
                     TGLESYDVKIKIM"
     gene            complement(20562..21635)
                     /gene="yccM"
                     /locus_tag="ACHKTW_002087"
     CDS             complement(20562..21635)
                     /gene="yccM"
                     /locus_tag="ACHKTW_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFO6463112.1"
                     /translation="MAEKKRTRWQRRPGTTGGKLPWNDWRNATTWRKATQLLLLAMNI
                     YIAITFWYWVRYYETASSTTFVARPGGIEGWLPIAGLMNLKYSLTTGQLPSVHAAAML
                     LLVAFIVISLLLKKAFCSWLCPVGTLSELIGDLGNKLFGRQCVLPRWLDIPLRGVKYL
                     LLSFFLYIALLMPAQAIHYFMLSPYSVVMDVKMLDFFRHMGTATLISVTVLLIASLFI
                     RHAWCRYLCPYGALMGVVSLLSPFKIRRNAESCIDCGKCAKNCPSRIPVDKLIQVRTV
                     ECTGCMTCVESCPVASTLTFSLQKPAANKKAFALSGWLMTLLVLGIMFAVIGYAMYAG
                     VWQSPVPDELYRRLIPQAPMIGH"
     gene            complement(21707..24451)
                     /gene="torS"
                     /locus_tag="ACHKTW_002088"
     CDS             complement(21707..24451)
                     /gene="torS"
                     /locus_tag="ACHKTW_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
                     /protein_id="HFO6463113.1"
                     /translation="MNLTLTRRLWMGFALMALLTLTSTLVGWYNLRFISQVEKDNTQA
                     LIPTMNMARQLSEASAWELFAAQNLTSADNEKMWQAQGRMLTAQSLKINALLQALREQ
                     GFDTTAIEQQEQEISRSLRQQGELVGRRLQLRQQQRQLSQQIVAAADEIARLAQGQAN
                     NATTSAGATQAGIYDLIEQDQRQAAESALDRLIDIDLEYVNQMNELRLSALRVQQMVM
                     NLGLEQIQKNAPTLEKQLNNAVKILQRRQIRIEDPGVRAQVATTLTTVSQYSDLLALY
                     QQDSEISNHLQTLAQNNIAQFAQFSSEVSQLVDTIELRNQHGLAHLEKASARGQYSLL
                     LLGMVSLCALILILWRVVYRSVTRPLAEQTQALQRLLDGDIDSPFPETAGVRELDTIG
                     RLMDAFRSNVHALNRHREQLAAQVKARTAELQELVIEHRQARAEAEKASQAKSAFLAA
                     MSHEIRTPLYGILGTAQLLADNPALNAQRDDLRAITDSGESLLTILNDILDYSAIEAG
                     GKNVSVSDEPFEPRPLLESTLQLMSGRVKGRPIRLATAIADDMPCALMGDPRRIRQVI
                     TNLLSNALRFTDEGYIILRSRTDGEQWLVEVEDSGCGIDPAKLAEIFQPFVQVSGKRG
                     GTGLGLTISSRLAQAMGGELSATSTPEVGSCFCLRLPLRVATAPVPKTVNQAVRLDGL
                     RLLLIEDNPLTQRITVEMLNTSGAQVVAVGNAAQALETLQNSEPFAAALVDFDLPDVD
                     GITLARQLAQQYPSLVLIGFSAHVIDETLRQRTSSLFRGIIPKPVPREVLGQLLAHYF
                     QLQVNNDQSLDVSQLNEDAQLMGTEKIHEWLVLFTQHALPLLDEIDIARASQDSEKIK
                     RAAHQLKSSCSSLGMRIASQLCAQLEQQPLSAPLPHEEITRSVADLEAWLNKKDLNAI
                     "
     gene            24534..25562
                     /gene="torT"
                     /locus_tag="ACHKTW_002089"
     CDS             24534..25562
                     /gene="torT"
                     /locus_tag="ACHKTW_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HFO6463114.1"
                     /translation="MRVLLFLLLSLFMLSAFSADNLLRWHDAQHYTVQASTPLKAKRA
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVANLPVIELVNALDDPLVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            complement(25535..26227)
                     /gene="torR"
                     /locus_tag="ACHKTW_002090"
     CDS             complement(25535..26227)
                     /gene="torR"
                     /locus_tag="ACHKTW_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HFO6463115.1"
                     /translation="MPHHIVIVEDEPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDNCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            26357..27529
                     /gene="torC"
                     /locus_tag="ACHKTW_002091"
     CDS             26357..27529
                     /gene="torC"
                     /locus_tag="ACHKTW_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HFO6463116.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHAKQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNQCHG
                     APEIAHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKEEK"
     gene            27529..30075
                     /gene="torA"
                     /locus_tag="ACHKTW_002092"
     CDS             27529..30075
                     /gene="torA"
                     /locus_tag="ACHKTW_002092"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0050626 - trimethylamine-N-oxide reductase
                     (cytochrome c) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
                     /protein_id="HFO6463117.1"
                     /translation="MNNNDLFQASRRRFLAQLGGLTVAGMLGPSLLTPRRATAAQAAT
                     DAVISKEGILTGSHWGAIRATVKDGRFVAAKPFELDKYPSKMIAGLPDHVHNAARIRY
                     PMVRVDWLRKRHLSDTSQRGDNRFVRVSWDEALDMFYEELERVQKTHGPSALLTASGW
                     QSTGMFHNASGMLAKAIALHGNSVGTGGDYSTGAAQVILPRVVGSMEVYEQQTSWPLV
                     LQNSKTIVLWGSDLLKNQQANWWCPDHDVYEYYAQLKAKVAAGEIEVISIDPVVTSTH
                     EYLGREHVKHIAVNPQTDVPLQLALAYTLYSENLYDKNFLANYCVGFEQFLPYLLGEK
                     DGQPKDAAWAEKLTGIDAESIRGLARQMAANRTQIIAGWCVQRMQHGEQWAWMIVVLA
                     AMLGQIGLPGGGFGFGWHYNGAGTPGRKGVILSGFSGSTSIPPVHDNSDYKGYSSTIP
                     IARFIDAILEPGKVINWNGKSVKLPPLKMCIFAGTNPFHRHQQINRIIEGWRKLETVI
                     AIDNQWTSTCRFADIVLPATTQFERNDLDQYGNHSNRGIIAMKQVVPPQFEARNDFDI
                     FRELCRRFNREEAFTEGLDEMGWLKRIWQEGVQQGKGRGVHLPAFDDFWNNKEYVEFD
                     HPQMFVRHQAFREDPDLEPLGTPSGLIEIYSKTIADMNYDDCQGHPMWFEKIERSHGG
                     PGSQKYPLHLQSVHPDFRLHSQLCESETLRQQYTVAGKEPVFINPQDASARGIRNGDV
                     VRVFNARGQVLAGAVVSDRYAPGVARIHEGAWYDPDKGGEPGALCKYGNPNVLTIDIG
                     TSQLAQATSAHTTLVEIEKYNGAVEQVTAFNGPVEMVAQCEYVPASQVKS"
     gene            30072..30671
                     /gene="torD"
                     /locus_tag="ACHKTW_002093"
     CDS             30072..30671
                     /gene="torD"
                     /locus_tag="ACHKTW_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051259 - protein complex oligomerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HFO6463118.1"
                     /translation="MTTLTAQQIACVYAWLAQLFSRELDDEQLTQIASAQMAEWFSLL
                     KSEPPLAAAVNELENCIATLTVRDDARLELAADFCGLFLMTDKQAALPYASAYKQDEQ
                     EIKRLLVEAGMETSGNFNEPADHLAIYLELLSHLHFSLGEGTVPARRIDSLRQKTLTA
                     LWQWLPEFVVRCRQYDSFGFYAALSQLLLVLVESDHQNR"
     gene            complement(30764..31069)
                     /gene="cbpM"
                     /locus_tag="ACHKTW_002094"
     CDS             complement(30764..31069)
                     /gene="cbpM"
                     /locus_tag="ACHKTW_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HFO6463119.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(31069..31989)
                     /gene="cbpA"
                     /locus_tag="ACHKTW_002095"
     CDS             complement(31069..31989)
                     /gene="cbpA"
                     /locus_tag="ACHKTW_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HFO6463120.1"
                     /translation="MELKNYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNRQFHHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPAARGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGAKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
     gene            32250..>32567
                     /locus_tag="ACHKTW_002096"
     CDS             32250..>32567
                     /locus_tag="ACHKTW_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6463121.1"
                     /translation="MGSNIHGISCTANNYLKQAWNDIKNEYEKNQTYSITLFENTLVC
                     FMRLYNELRRKVNEEDTPCLECESLEKEFEEMQNDNDLSLFMRTLRTNDTQIYSGVSG
                     GITY"
BASE COUNT         7760 a         8297 c         8550 g         7960 t
ORIGIN      
        1 accaaaggga tggaaatcca cgttcgccag cgcaccatga agaaggagct ggaaaagctg
       61 accagtgcgg atgagatact ggcatacagg gttggctggg cacaggaata acagacaaaa
      121 agatggggga ccatcacctc ccccatgaac tgcgttgatt atgtaattat tgtgaagaga
      181 ccttatctac acagataaag agtgccacgg aaaagttaat cctgaaagtg ataccggtct
      241 catttccaga gcatcctgtt gatatggatg atttttcaga tttccggaag tccgtatttt
      301 gcccgtgcgc ggttacatgc ttcattcgcg atgccatttt cgccggacat tgcgaattcc
      361 ctgcatgtgg acggacggtt tttgtaaaca gagcaatatg cgttttcacc cggagttccc
      421 gccagagcga ctgaccggaa cctttccgcc agcatcgtca gtttctgccc agtaaaaaga
      481 gacgcggaaa atgcacaaca ggcaccacac gtcatgcatg gattggggtt attcataatt
      541 catcggtata gcgataaatc ataaagagaa aaatctatct ggtatgtgtt gagctaaata
      601 taccaattag tataccaagt gtatactaag agcaaaaaaa gaaaggggct accatttggt
      661 aaccccttga ttgtttggcg gaagcgcaga gattcgaact ctggaaccct ttcgggtcgc
      721 cggttttcaa gaccggtgcc ttcaaccgct cggccacact tccggaatga cgcgcactat
      781 aaacatcccg atgcggcgtg taaaccccta atttgtttgt ttgcctgaaa aacagccaaa
      841 agtgcattga tagcgtgaaa taacagcaga ttgatcattt catcaccatg aattccttct
      901 cttttactcg tttagcaacc ggctaaacat ccccaccgcc cggccaaaag aaaaataggt
      961 ccatttttat cgctaaaaga taaatccaca cagtttgtat tgttttgtgc aaaagtttca
     1021 ctacgcttta ttaacaatac tttctggcga cgtgtgccag tgcagaagga tgagctttcg
     1081 ttttcagcat ctcacgtgaa gcgatggttt gccttgctac agggacgtcg cttgccgacc
     1141 ataagcgccc ggtgtcctgc cggtgtcgca aggagaagag acgtgcgata tgaataacga
     1201 ggaaacattt taccaggcca tgcggcgtca gggcgttacc cggcgcagct ttctcaaata
     1261 ttgtagtctg gctgccacgt cgctgggatt aggcgcggga atggcaccaa agattgcctg
     1321 ggcgctggag aacaaacccc gcattccggt ggtatggatc cacggtctgg aatgcacctg
     1381 ctgtaccgaa tcttttatcc gctccgctca cccactggcg aaggacgtca tcctttccct
     1441 gatttccctc gattatgacg atactttgat ggctgccgcc ggaacccagg cggaagaagt
     1501 cttcgaagac atcatcacgc aatacaatgg caaatatatc ctcgcagtag aaggtaatcc
     1561 gccgctgggc gagcagggga tgttctgtat cagcagcggt cgaccgttta ttgagaaact
     1621 caaacgtgcc gctgccggag ccagcgcgat tatcgcctgg ggaacctgcg cgtcctgggg
     1681 ctgcgtgcag gccgcgcggc ccaatccgac gcaggcaacg cctatcgaca aagtcatcac
     1741 cgacaaaccc attatcaaag tacctggctg cccgccgatc ccggatgtga tgagcgccat
     1801 cattacttac atggtgacct ttgatcgctt gccagatgtc gacagaatgg gccgtccgct
     1861 gatgttctat ggtcagcgaa tccacgataa atgctatcgc cgcgcccact tcgacgccgg
     1921 agagttcgtc cagagttggg atgatgacgc tgcccgcaaa ggttactgcc tgtacaaaat
     1981 gggctgcaaa gggcctacca cctataacgc ctgttcctcc acacgctgga atgatggcgt
     2041 ttctttccca atccagtctg gtcacggctg cctgggctgt gcggaaaatg gtttctggga
     2101 tcgcggttcg ttctacagcc gcgtggtcga tattccgcaa atgggtactc attccaccgc
     2161 cgataccgtc ggtttaaccg cgcttggcgt ggtggcagcg gctgttggtg tgcacgcagt
     2221 cgccagcgcc gttgaccagc gcagacgtca taaccagcaa cctacagaaa ccgaacatca
     2281 gccaggcaat gaggataaac aggcatgagc actcagtacg aaactcaggg atacaccatc
     2341 aataatgccg gacgccgcct ggtggtcgac ccgattacgc gcatcgaagg ccacatgcgc
     2401 tgcgaagtga atattaacga tcagaatgtg atcaccaatg ccgtctcctg cggcaccatg
     2461 tttcgcgggc tggagatcat cctacaaggg cgcgacccgc gcgatgcgtg ggcgttcgtt
     2521 gaacgtatct gcggcgtctg tactggcgta cacgccctgg cttcggttta cgccatcgaa
     2581 gatgctatcg gtattaaagt gccggacaac gccaatatca tccgcaacat tatgctggca
     2641 acgctctggt gccacgatca tctggtgcac ttctatcagc ttgccgggat ggactggatc
     2701 gatgtgttag atgcgctgaa agccgacccg cggaaaacct ccgaactggc gcaaagtctc
     2761 tcctcttggc cgaaatcatc ccctggctat ttcttcgacg tacaaaaccg cctgaaaaaa
     2821 tttgttgaag gcgggcagtt ggggatcttc cgcaatggct actgggggca cctgcagtac
     2881 aaactgccgc cagaagctaa cctgatgggc tttgcccact atctcgaagc tctcgatttc
     2941 cagcgtgaaa ttgtcaaaat ccacgcggtc tttggcggta aaaacccgca tccaaactgg
     3001 attgtcggcg ggatgccttg cgccatcaac attgacgaaa gcggcgcggt cggggcagtc
     3061 aatatggaac gcctgaacct ggtgcagtca attatcaccc gcacggcgga cttcattaac
     3121 aacgtgatga tccccgacgc cttagccatc ggtcagttca acaaaccgtg gagcgaaatc
     3181 ggcactggtc tttctgataa atgcgttctc agctacggcg cattcccgga tattgccaac
     3241 gactttggcg agaaaagtct gctgatgcct ggcggcgcgg tgattaacgg cgacttcaac
     3301 aatgtgctgc cagtggattt ggttgatccg cagcaggtgc aggagtttgt cgaccacgcc
     3361 tggtatcgat atcccaacga tcaggtcggg cgtcatccgt tcgatggcat caccgacccg
     3421 tggtacaacc ccggcgatgt caaaggcagc gataccaaca ttcagcagct gaatgaacag
     3481 gaacgctact cgtggatcaa agcgccacgc tggcgcggta acgcgatgga agtggggccg
     3541 ctggcgcgca cgttaatcgc ttatcacaaa ggcgatgctg cgaccgttga gtcggtcgat
     3601 cgcatgatgt cggcgttgaa cctgccgctt tccggtatcc agtcaacgtt aggccgcatt
     3661 ttgtgccgcg cgcacgaagc gcagtgggcc gcaggtaagt tgcagtattt cttcgacaag
     3721 ctgatgacca acctgaaaaa cggcaatctc gccactgctt ccacggaaaa atgggaacct
     3781 gcaacctggc cgacagagtg ccgtggtgtc ggttttaccg aagcgccgcg cggggcgtta
     3841 ggccactggg ccgccattcg cgatggcaag attgatctct accagtgcgt ggtgccgacc
     3901 acctggaacg ccagcccgcg cgatcccaaa gggcagattg gcgcttatga agcggcgctg
     3961 atgaacacca aaatggcgat ccccgagcaa ccgctggaga tcctgcgtac tctgcacagc
     4021 tttgacccgt gcctcgcctg ttcaacacac gtgctgggcg acgacggtag cgagctgatc
     4081 tccgtgcagg tgcgttaaca gcgaaggaga atcatcatgc aacagaaaag cgacaacgtt
     4141 gtcagccact atgtctttga agcgccagtc cgcatctggc actggttgac ggtgttatgc
     4201 atggcggtgt tgatggtcac cggatacttt atcggcaagc cgctaccttc cgtcagcggc
     4261 gaggcgacgt atctgttcta tatgggctac atcaggttaa ttcacttcag cgccgggatg
     4321 gtttttaccg tggttttgct gatgcggatc tactgggctt ttgttggcaa tcgatactcc
     4381 cgcgagctgt ttatcgtgcc ggtatggcgt aaaagctggt ggcagggcgt gtggtatgaa
     4441 atccgctggt atctgtttct ggcaaaacgt ccgagtgccg atataggcca taatcccatc
     4501 gcccaggcgg cgatgttcgg ctatttcctg atgtcggtct ttatgatcat cactggtttt
     4561 gcgctgtaca gcgaacacag ccagtacgct atttttgcgc cgttccgtta tgtggtggaa
     4621 tttttctact ggacgggtgg caactcaatg gacattcaca gctggcatcg gctggggatg
     4681 tggctgattg gcgcgtttgt gatcggtcat gtctacatgg cgctgcgtga agacatcatg
     4741 tccgacgaca cggtgatctc caccatggtc aacggctacc gtagccacaa atttggcaaa
     4801 ataagtaaca aggagcgttc atgagcgagc aacgcgtggt ggtcatgggg ctgggcaacc
     4861 tgctgtgggc cgatgaaggc ttcggcgtgc gggtggcgga acggctgtat gcccattacc
     4921 actggcccga gtatgtggag attgtcgatg gcggtactca gggactgaac ttgctggggt
     4981 atgtcgaaag cgccagccat ctgttgattc tcgatgccat tgactacggg ctggaacctg
     5041 gaacgctgcg aacctatgcc ggagaacgca ttccggctta tctcagcgcg aagaaaatga
     5101 gcctgcatca gaacagtttc tccgaagtgc tggcgctggc agatatccgc ggacatctgc
     5161 cagcacatat tgccctcgtc ggtctgcaac ccgctatgct cgacgactac ggcggcagcc
     5221 tgagcgaact ggcacgggat caactgcccg ctgcggaaca atcggcgttg gcgcaactgg
     5281 ctgcgtgggg aattgtgccg caaccggcta atgaatcgcg ctgtctcaat tatgactgtc
     5341 tgtcgatgga aaattacgaa ggcgttcgtt tgcgccagta caggatgaca ctggaggagc
     5401 agggatgagc aacgacacgc catttgatgc gttgtggcaa cgaatgctgg cgcgcggctg
     5461 gacgccggtc agtgaatccc gtcttgacga ctggcttacg caagcgccag acggcgtggt
     5521 gttattaagc agtgacccga aacgtacgcc agaggttagc gataatccgg taatgattgg
     5581 cgaattactg cgcgagtttc ccgactatac atggcaggtg gcgattgctg accttgagca
     5641 gagcgaagcc atcggcgatc gctttggcgt ctttcgcttt cccgccacat tagtgtttac
     5701 cggcggaaac tatcgcggtg tgctgaatgg tattcacccg tgggcggaac tgataaacct
     5761 gatgcgcggg cttgtcgaac cgcagcagga gcgtgcctca tgagcgaaac ttttttccat
     5821 ctgctggggc caggaacgca accgaacgat gacagtttca gcatgaatcc actgccgatc
     5881 acctgtcagg tgaatgatga accgagtatg gcggccctgg aacaatgtgc tcacagcccg
     5941 caggtgattg cgctgttaaa cgagttacaa catcaaatga gtcaacgcca gccgccgttg
     6001 ggcgaggtgc tggcagtcga tctgttaaat ctccacgccg acgatcgtca ctttatcaat
     6061 acccttctcg gggaagggga agtgtcagtg cgcattcagc aggctgacga cagtgaaagt
     6121 gaaatacagg aggcgatctt ctgcggatta tggcgggtgc gcagacgtcg cggcgaaaag
     6181 ttgctggagg acaaactgga agctggctgt gcgccgctgg cgttgtggca ggcggcaacg
     6241 caaaatctct tgccgacaga ttcgctgtta ccgccgccca ttgatggcct gatgaatggc
     6301 ctaccgttgg cgcatgagtt actggcacat gtacgtaacc ccgacgcgca gccgcacagc
     6361 attaatctga cgcaattacc catcagcgag gctgatcggc tttttctctc acgtctctgt
     6421 gggccgggaa atattcagat tcgtaccatt ggctatggcg agagctatat caacgccacg
     6481 gggttacgcc atgtctggca tttacgctgt acggacacct taaaaggccc tttactggaa
     6541 agttatgaaa tctgcccgat accggaagtg gtgctggcag cgccagaaga tttggtcgac
     6601 tctgcgcagc ggcttagcga ggtatgtcag tggctggcgg aagctgcacc gacgtaaaaa
     6661 gacggtaagt atcgctttca gtcttatgaa tatcgcaatc ggcgaatacc tctggtcgta
     6721 gagtttcagg ataaagaggg agatctacca ttatcgggtt atttttctct cttcgcctac
     6781 aggagtgcgc atgtgggatg tcattgattt atcgcgctgg cagtttgctc tgaccgcgct
     6841 gtatcacttt ttatttgtac cccttaccct ggggctgatt tttttgctgg ctatcatgga
     6901 aaccatttac gtggtcaccg gcaaaacaat ctaccgcgat atgacgcgct tctggggtaa
     6961 gctcttcggt atcaattttg ctcttggcgt ggctaccggc ctgaccatgg agtttcagtt
     7021 tggtactaac tggtcattct attccaacta tgtgggcgat atttttggcg caccactggc
     7081 gatggaagca ttaatggcct tcttcctcga atccaccttt gtcgggctgt tcttcttcgg
     7141 ctggcaacgg ctgaataaat accagcacct gctggtgacg tggctggtgg cgttcggttc
     7201 aaatctctct gcgttgtgga tattgaatgc caacggttgg atgcaatacc cgaccggtgc
     7261 gcattttgat atcgacaccc tgcgtatgga gatgaccagc ttcagcgagc tggtctttaa
     7321 cccggtcagc caggtgaaat ttgtgcacac cgtaatggcg ggctacgtga ccggggccat
     7381 gtttattatg gcgatcagcg cctggtattt actgcgcgga cgggagcgcg atgtcgcatt
     7441 acgttcgttt gccatcggtt ccgtcttcgg tactctggcg attatcggta ccctgcaact
     7501 cggagacagt tctgcgtatg aagtcgcgca agtacaaccg gtaaaactgg cggcgatgga
     7561 aggggagtgg caaacggaac ctgcacctgc accgttccat gtggttgcct ggccggaaca
     7621 ggatcaagag cgtaacgcct ttgccatcaa aattcccgcg ctgctaggga tcctcgccac
     7681 tcactcatta gataaacccg tgccgggtct gaagaatttg atggctgaaa cctacccacg
     7741 cttgcaacgc ggacgtatgg cctggctgtt aatgcaggaa atatcgcaag gcaatcgtga
     7801 gccgcatgtg ttgcaggcat tccgggaact ggaaggtgac ctgggctacg gcatgttgct
     7861 ctcccgctat gcgccggata tgaatcatgt cacagccgca cagtaccagg cggcgatgcg
     7921 tggcgcgata cctcaggttg cgccggtatt ctggagtttc cgcatcatgg tgggctgtgg
     7981 ttccctgctg ctactggtga tgctgattgc gcttgtccag acgctgcgtg gcaaaatcga
     8041 ccagcatcgc tgggtgctga aaatggcgct ctggagtttg ccgttgccgt ggattgcgat
     8101 tgaagccggg tggtttatga ccgagtttgg tcgtcagccg tgggcgatac aggacatctt
     8161 accgacatac tccgcgcact ccgctttaac cacaggacaa ctggctttct cactgatcat
     8221 gatcgtaggg ctttacaccc tgttcttaat cgccgaagtc tacctgatgc agaaatatgc
     8281 ccgtctgggg ccgagcgcga tgcagagtga acaaccgacg cagcaacagg ggtaaaggag
     8341 aaaatcatgt ttgattatga aacattgcgc ttcatctggt ggctgctgat tggcgtgatc
     8401 ctggtggtct ttatgatctc cgacggattt gacatgggga tcggctgtct gctgccgctg
     8461 gtggcgcgta atgatgatga acgccggata gtgataaaca gcgttggtgc acactgggaa
     8521 ggcaaccagg tctggttgat cctcgctggt ggggcattat ttgccgcctg gcccagagtg
     8581 tatgcagcgg cgttttccgg cttttatgtg gcgatgatcc tggtgctgtg ctcgctgttc
     8641 ttccgtccgc tggcctttga ttatcgcgga aaaattgccg atgcacgctg gcgtaaaatg
     8701 tgggatgccg gtctggtcat cggcagtctg gtgccgccgg tagtcttcgg tatcgccttc
     8761 ggcaacttgt tgctcggcgt gccgtttgcc ttcacaccgc aattacgcgt ggagtatctc
     8821 ggcagcttct ggcaactgct gacgccattc cctttattgt gcggattgct cagccttggg
     8881 atggtgattt tgcaaggtgg cgtctggtta caactgaaaa ctgttggtgt gattcatctg
     8941 cgttcacagc tggcgactaa acgcgctgca ctgttggtga tgctgtgctt tttgctggcg
     9001 ggttactggc tgtgggtcgg tattgatggc tttgtactgc tcgcccagga tgctaacggt
     9061 ccttccaatc cgttaatgaa actggtggca gtgctacctg gtgcctggat gaataatttc
     9121 gtcgaatcgc ccgttttgtg gatcttcccg ctgctggggt tcttttgccc attgctgacg
     9181 gtgatggcga tttatcgtgg tcgcccgggg tggggatttt tgatggcgtc attgatgcaa
     9241 tttggcgtga ttttcacggc aggcatcacg ctgttcccct tcgtcatgcc gtcaagcgtg
     9301 agtccgatct ccagcctgac gttgtgggac agcacttcca gccagctgac gctgagcatt
     9361 atgttggtaa tcgtgctgat atttttgccc attgtgttgc tctacactct ctggagctac
     9421 tacaaaatgt gggggcgcat gacaacagaa actctccgcc gtaacgaaaa cgagttgtac
     9481 taaggagcag gaacaatgtg gtatttactt tggttcgtcg gcattttgtt gatgtgttcg
     9541 ctctccaccc ttgtgttggt atggctggac ccgcgtctga aaagttaacg aacgtaggcc
     9601 tgatgcggcg cattagcatc gcatcaggca atcaataatg tcagatatga aaagcggaaa
     9661 catatcgatg aaagcgatct taatcccatt tttatctctt ctgattccgt taaccccgca
     9721 atctgcattc gctcagagtg agccggagct gaagctggaa agtgtggtga ttgtcagtcg
     9781 tcatggtgtg cgtgctccaa ccaaggccac gcaactgatg caggatgtca ccccagacgc
     9841 atggccaacc tggccggtaa aactgggttg gctgacaccg cgcggtggtg agctaatcgc
     9901 ctatctcgga cattaccaac gccagcgtct ggtagccgac ggattgctgg cgaaaaaggg
     9961 ctgcccgcag tctggtcagg tcgcgattat tgctgatgtc gacgagcgta cccgtaaaac
    10021 aggcgaagcc ttcgccgccg ggctggcacc tgactgtgca ataaccgtac atacccaggc
    10081 agatacgtcc agtcccgatc cgttatttaa tcctctaaaa actggcgttt gccaactgga
    10141 taatgcgaac gtgactgacg cgatcctcag cagggcagga gggtcaattg ctgactttac
    10201 cgggcatcgg caaacggcgt ttcgcgaact ggaacgggtg cttaattttc cgcaatcaaa
    10261 cttgtgcctt aaacgtgaga aacaggacga aagctgttca ttaacgcagg cattaccatc
    10321 ggaactcaag gtgagcgccg acaatgtctc attaaccggt gcggtaagcc tcgcatcaat
    10381 gctgacggag atatttctcc tgcaacaagc acagggaatg ccggagccgg ggtggggaag
    10441 gatcaccgat tcacaccagt ggaacacctt gctaagtttg cataacgcgc aattttattt
    10501 gctacaacgc acgccagagg ttgcccgcag ccgcgccacc ccgttattgg atttgatcat
    10561 ggcagcgttg acgccccatc caccgcaaaa acaggcgtat ggtgtgacat tacccacttc
    10621 agtactgttt attgccggac acgatactaa tctggcaaat ctcggcggcg cactggagct
    10681 caactggacg cttcccggtc agccggataa cacgccgcca ggtggtgaac tggtgtttga
    10741 acgctggcgt cggctaagcg ataacagcca gtggattcag gtttcgctgg tcttccagac
    10801 tttacagcag atgcgtgata aaacgccgct gtcattaaat acgccgcccg gagaggtgaa
    10861 actgaccctg gcaggatgtg aagagcgaaa tgcgcagggc atgtgttcgt tggcaggttt
    10921 tacgcaaatc gtgaatgaag cacgcatacc ggcgtgcagt ttgtaatgca taaaaaagag
    10981 cattcagtta cctgaatgct ctgaggctga tgacaaacga agaactgtct aatgcgtaga
    11041 ccggaaaagg cgttcacgcc gcatccggcc actttcagtt ttactctttc tcggagtaac
    11101 tataaccgta atagttatag ccgtaactgt aagcggtgct ggcgcgttta atcacaccat
    11161 tgaggatagc gcctttaata ttgacgcctg cctgttccag acgctgcatt gacaaactca
    11221 cctctttggc ggtgttcaag ccaaaacgcg caaccagcag gctggtgcca acagaacgcc
    11281 ccacgaccgc ggcatcactc accgccagca tcggcggcgt atcgacaatc accagatcgt
    11341 aatggtcgtt cgcccattcc agtaattgac gcatccgatc gcgcatcagc agttcagacg
    11401 ggttaggtgg cacctgaccg cgagtaatca catcaaagcc tcctttgcca aaatgctgga
    11461 tcactttgtt gagctcatct ttacctgcca gatattccga caagccatgt tcattactca
    11521 cggtaaacag gttatgcgaa taaccacggc gtaagtcggc atcaataaat aacacttttt
    11581 gatcggactg ggcgatcacc gctgccagag ttgaactgac aaacgtttta ccactgtctg
    11641 gcgtcgcacc ggtgatcatc agaatgttat tctccgtctc catcatagcg aaatgcagac
    11701 tggttcgtag cgcacgtacg gcttccacag cagaatccgc cgggttatcc accgccagga
    11761 aggggatatt tttagtacga tggcgctgct gattagaaaa taaatttttc ttacgcagac
    11821 gggtgcgttt atccagccac tcggacattg ggatagtggc ataaacgctg atgccgtgct
    11881 cttccagttg ttccggggct tctacaccac gacgcaacat cgcacgcgcc agcacggcac
    11941 ccacagaaat aaacaggcca agaataaaac caagcaccac attcaacgct tttttcggtt
    12001 tcactggctg cggctgagtg actgccgggt cgataatccg cacgttacca atggcactgg
    12061 atttcgaaat actcaactcc tgctggcggt taagtaattg cagatatacc gcacggcccg
    12121 cttctacgtc acgacttaaa cgcaacactt cctgttgggt ggaaggcatt gccgataccc
    12181 gcttattcag gcgtttgcgt tcttgctcca gcgtctggcg tttttccagc agcgcacgat
    12241 aagttgggtg atctttctta tacagctggg agatctctgc ctcgcggaag gtcagctcat
    12301 tgagttgatt atcaacgttc acaatctgct caagaacggc tttggcttcc aggttaaggt
    12361 caaccgaatc gcgctgctgg cgataaacgt tgagtttttc ttccgcctgg tccagctcgc
    12421 tgcgcacttc aggtaactgg cgctgtaaga attcaaggct ttgtgaatcc tgcgccgcct
    12481 ggcgagcgat attctgttgc aaatagttgt tagcgatgct gttcagaata cgagtaatca
    12541 actggggatc atcaccagtc atggtaagtt ccagcatccc gctttcttta ctgcgttcgc
    12601 taacggtaaa ggtttcctgc aatgcgttaa tcgcttccag ttcggtacgc tggctcagga
    12661 caaactgtgt tcctggtttg gccttaatgt ccgcgatagt cagcgcaacg ccatcttttt
    12721 ccagacgctg gccgaccata ccattgacgg tgaactcttc accttccagt gtatagtggc
    12781 cgttttcccc aaccgtgagt gtcagttgct gatcctgacc attcagttgt ggaatatgca
    12841 tccagctgat cgccagctca cctggttttt ctttggttaa tctcgcccag ccgcgaccca
    12901 caatcggaaa atacttctgc tcaactatgt cgcgcagatt cagttcagca atggttttac
    12961 cgagaatcat gcgcgattgc agcagttgga tctccggtgc agactcgggc gatgaattag
    13021 ggatcatatc gctcaggccg ctgagaatgg cgttgccctg tttttgctca acctggacca
    13081 gagtatctgc ctgataaatt ggtgtactta acagcgagta agcgacagcg atcagcgtga
    13141 ataacgcggt cacgctgata ataaacttac ggtgatccca taactcgccg accagacgaa
    13201 gcagatcgat ctcattttcc tgagtgctgc ctggtggcgt attcatattt ttagttgtca
    13261 tcgtatttta taaactcaaa ttaccggctg aggcgcttcg cccattcctg actggcgcgc
    13321 tccaacatac cgtagacatg ttcaaatgcg tcctgacttt tacgataggg atccgggatc
    13381 tctttctgtt ccagccattg cccaaacagc attgtcttcc cgcggacctc gggagcaatt
    13441 gcggtaacct gagcaatatg ttccgactcc attgccagaa tcagatcgta atttcgtgcc
    13501 atctcagcag tgagcttacg tccggcatgt ccttcaagag atacgccatg attagcggca
    13561 acatctgccg ctgtcgcatc tgcagggtgt tttaccagac catgaacacc tgccgatttg
    13621 acttttacgc ccggcagacg tttacgcagc aagcgctcgc caatgggcga acggcaaatg
    13681 ttgccggtac aaaccaccag gattgagtta aattttagtt gggccatgtt tgaatccacc
    13741 gtgaagtctc tgtcaggtca tgcaccccgg aaatcgtcgg taccagcaga gaaatcacac
    13801 ggttccaacg agccagcggc gcggtagtga cataaacaat gtcgtaaggt tcaagctgga
    13861 attccgttcc gagtatcatt gccgatgcgt ccttcgcatt cagctgataa atattggcga
    13921 ttttgccatt ctgcttgttt tgcgttgcac gaataacaaa aatccccgtc gcatcagcga
    13981 catcctgatt cataccctca gcgttaccca gcgcttccgc cagggtcata ccgctgcggt
    14041 ccattttcag cgtgctctgt ttacccactt cacccatcac gaagactttc agatcatcgt
    14101 tgcgcggaat aaataaaata tcgcccgggt gcagcagttt gttctggcgc aagtcgccac
    14161 gctgcatcag cgcatagagg ttcactttgg tctttacgcc attctgggtg agcaccacgt
    14221 tgcgccagtc tgcatctgcg gtcagaccgc ccgcggcgtt aatcgcatcc atgatggtca
    14281 gaggaatatt ggtaatcggc tgctgacccg atttgctgac ttcgccggtg acgtacgctt
    14341 tttgcgagcg gaacgccgcg acgctgacat cgacttgcgg gctttcaatg actgaatcca
    14401 gacgcgctgt aatctcgttg cgtacctggg taagtgtttt cccggccact ttcaggcgac
    14461 caatataggg gtagaaaatc gcaccatcgg cgttaaccca gttgccggta tcactggcgc
    14521 tacggtattg ccctgcgggg gtggttagtt ccgggtgatc ccagaccgta accatcaaca
    14581 catccccaat gccgatacga tattcatagt ttgcgatttg ctgctccagc tccgggttag
    14641 cctgcgacat aatcggtttg gcgcgcaact gatcaatcaa tcctggcgtg acgggataga
    14701 tattgaccat tttgtccagg tcatactgat tgtcaggtag ttcaatgaca tccttgttac
    14761 tggtcgataa attctgacca ggaacaaggg tacatgctgt caaagacaga acagccaatg
    14821 tcagtaccga aaacttaaaa atgttctttt tcattggttt atatttatca gtcaatgtaa
    14881 tgttatgaat tacgtcaggt tattgtccaa ccggtacttc tcgctctgcc gtcattggat
    14941 agagctggta tttgcgtgac agcatttgtc caccatcacg cgtcaatggt gtccacgaaa
    15001 ccaccgcgcg gttgcggtta ggtccaatgg tcatcaagtc gaatggaatc gagatataaa
    15061 aacctttact aaagccgcct tcgccgtagt catctttaga tacgttactg attgccgccc
    15121 atacccctac cgccacgccg ctgtcaaagc gtttggcgac gtcgatcgtt gcgcctttat
    15181 cttttgccag atattgccca acgctaagtt tcatcagtac gccattgagc gtcggcgggt
    15241 tccagtaagc cgtcacgaaa cctgttggcg tggaataatc ggtgaaacgc atcatgttgt
    15301 cccagtcacg ttgcttcacg tagttaacgt ccacacccag cgcccagctg gcatctagcg
    15361 ggcgataaag cagctcggaa ccgacaccgg cgtacatcgt ttccagataa ccgccataca
    15421 cctggccata gaaaccattg cctaagtcgg caaagtagtt cgcctgcaag ttgttgagat
    15481 aaacgtcatt gcgaacgtaa tcacggatat gcgtgcgcac gcggggcagg gtagagtccg
    15541 cgggcaacag cgaagactta aacttgtcgt agttgttgta aatattggtg aaaataccgc
    15601 catcaagcag caggtggtcg gtaaaccagt agcgggcact ggacatcagc cccagctgga
    15661 acatatagaa atcttccggg ccgcccagcg actgtgaaag tgttgggttg aaagagtagc
    15721 taaagcgatc ttcacgaata cggtagcccc gaccaaaggc agaaagatct tctgcttcaa
    15781 cacgttgttg ttgaagttgc tctgattgac caggcgctgt accagccagc tgtttgcgca
    15841 ggctggctac gtcggtttcg gtagtcacca tcgccatatg ctcgcgcttt tgcgtcacgc
    15901 taatcttctc aacgccttgc ggcaggttat tcaccagaat ccggttggca cgatcgaccg
    15961 cttcgcgaga atcacggtat ttgtattgtt gaccagacat atacagcgtc ttatcgcgca
    16021 gctgaatttc cggtgcttca aaacctgcgt tgtacttcag cgcggtaagt tggttcgcta
    16081 ctgtggtgta ctgcaatcct tcagattcag gcgcaggttg atatgccggt tttggcgtat
    16141 cgcgcagggc agggcgcaga tcgttgaaat tggtccgtaa cgtgaagcca aacatcaacg
    16201 tgttaccgcg ttcataactc aggttgagat ctgcccagct ggcagcgcga taaactgcgc
    16261 cgacgttgaa atggcttgcc tgaggcagtt tgccagcgaa atcattctgg taattgttgc
    16321 catcgtattc gagtttcaga cgcaggggat tccacggcgt ttgatactca atgccgccaa
    16381 agatggaagc cgggccacga aagatatcgc taaagctgat atcgcccgca tcgtgagact
    16441 ttgcgcgatg acaatattta tcgctcaccc ggcaaaacgg gttggtaata ttgcccgcat
    16501 taccggcgta gccccatgcc atcccgaggg tgaaatcaaa tggccccgct tgcttgctgg
    16561 ccaccagata ctcaccgtca aacagacccg taccagcaat atcacgttta ccaaacgcca
    16621 cttgcggtag ccagtaacct tcttcccaca ggcgaagttt aaaatcgaat gatttgtctt
    16681 tgtatgactg atcgccgctg aaatcttccc actggctata tttgcgtgtg cgcacatccg
    16741 tataacgaat ggtgccttcc agccatggga acagcgccac ggaggtggag tagaaccggt
    16801 attgatcgtt atcccggtag ttgacgctga attcaccttc cggtgcgatg cgcgcatttg
    16861 gcatctgcaa caatcctgtg ccgccaaagt ctgattgcga cgaacccagc ggatccgggt
    16921 aggttaatac ttcagcatga caggcactgg agacggcaat ggccaggcag cttaaaagat
    16981 aagaattctt cttcatcagt caggaacacg ttgcgttaaa acagacacaa tctggtcatt
    17041 taaatcagca tatttttcgg gcagtacatg cgcagaaaag cccaaccaaa gctggctgcc
    17101 aggcggtggt tccacatgtc ttttattcca cagggcaaca ggggcgacga cagtctcacc
    17161 ctcaggcgta atcaccataa cgttattctt gtccgcgcct gcaaggcggg ggtgatcttg
    17221 cagataatcg gtaacgctac ggcctgcctg ccagggcaat tgcccggccc cagaaaccgc
    17281 acccagcaga gtaatagtca ccggacgctg tacggtatag agcgtatagt ccccgaccaa
    17341 cgggggattg ctgttctcat ccactcgtac aaaatcaggg tccagtttga ccggtaagcg
    17401 accagtgata ttgagattga gtaactgctg gcgcacggat ttaatcgttg ccgccacatc
    17461 gtcgtcagct tctgcttccc aggaagcaag ctgtgccatg acatgttggt agtctttaag
    17521 cgctttagct ttcgccgcag agtcggtcag caaggcacca ggccaccaga ggcgatcgcg
    17581 cagttgcggt tgtgtcacca actggacaac attttccatg gggccaacgg aaagtgtctg
    17641 ttgttcgcca ggcagataaa tagtcaccgt tccttgcgca aaggcatggg gtgtcattac
    17701 gtaaagtacg ctggcaatga aatacgactg taatttattc atggtgcggg tttaagaaac
    17761 gtcatttcta cgggaataac ccctgcgcct aacatttgcc gactttgacg aacctggcca
    17821 gtagccgaat cgacccagaa tgtgttttgc cactgttttt ctggccgcgt ggaagtcacc
    17881 tcttcctgcc agacattaca cagcacattg cgacccgcaa tattcagcgt ctctaatcca
    17941 gcaaaggaaa aagtagaact cagggtcgca gagcgaaaat ggttgtcttc actccagaga
    18001 atatcgcgcg tccagcggct gccatctttt attgccagcg cgttacgcag agggtcctgc
    18061 ccggaatttg tcacttccag cagattatta ttaagtttga cggtttttaa aagacgtcca
    18121 ttgtgggtaa ccagcatggc gttatcctgg gataaccatt tgctttgttc ttgttctata
    18181 tatcccagta ccacaaagat tcgttgccct tcattaaggc gtaaatacat agtggaataa
    18241 ggcaacgcct ggatctgctg atccgctacg gtgatatcct gattatcgaa aaggctggcg
    18301 cgaaatgtat cgaccatact ttgctggctg tgcgtacatc ccgcaagaaa tagtgcgaaa
    18361 atcgataaaa taagagggcg cacctttaat ccttgaaagg ttaattaaaa aaatgcaaat
    18421 ctctaaagcc cggtacgaat atcgcaaccg ggtatcattt aactttaatt tcgacgcgtt
    18481 aaagcagtat tagcgtgtag tggacgtggt ggttgaggtg ttatgagaac cgccatcgcc
    18541 actgtcattt gctgcaacaa ctgccaacgt cgccacacca acagccgttg ccacaccgac
    18601 aactgtcgcc gtgccgacag cgccagcagc cacgccacca gtggtagtag cagtcgtggt
    18661 tgcgccggta gtggtcgttg tggtgccagt agtacccgtc gttgcctcgg tcccgtttgt
    18721 tgcttcaggg gcagcaaacg cagctggcgt cgtcaacatg aaggccataa ggattgcaga
    18781 aagcttgtgt ttcataactt ttcctttatt catcgcatgg acaatacgta cgcttgagat
    18841 caggattgtc tactttgagt ataacatcct tgtgaaatct ccctttcctc ataccgcttg
    18901 cactatatac tgcgggtagg aaaggcgcgc agagggaaat ataagattgt taactatccc
    18961 cctctggaaa tacgacttgt gagcataaga taaatcttag caaaatacaa attaaagata
    19021 aggaaattgt tacgaaagct attaattatt gttggtaata tagtttcaag tggtactata
    19081 actggctgtt aaatggcatt tataactatt aggtgcagag atattcgctt aaatggatta
    19141 gtttctttgt ggaaaagtaa ctgatgttat aatgtatgat gttgactatt gcactattcc
    19201 aataaaacca gtcagcttta aacaagcagc gtcatattaa gagagataaa catttgccgc
    19261 tgttggtcct cgcaggccat ttacgcggca aaattccaca cgtaatcctg gtataagcac
    19321 ttctgcgtcg cggggagtga atgcggaaat atggacctga acttctttac gaccgtcgga
    19381 ggggataatg aatcctttgc cgcttttgcg atcaaaggtt ttgacaattc ctgtcatttt
    19441 acgggacaaa caaattcctt actgaaaata ctgcgctgca ctatacgggg ttaataaaat
    19501 aaagccagcg atatttaaga ccgccggacg gctaaaataa aatttgctta atctcaatta
    19561 tcatgcgtta atagctgcgt cggtttgaaa gacagacagc atacaaagta gtttactaaa
    19621 gcagttctca ttatcaggca ttatcccctt cttttgagtc tctctcctga acactaagta
    19681 gtttctgtat taaagccctg tttgccgaaa ggcccaaaat gaaggaagta aaatatgtct
    19741 aataaaatga ctggtttagt aaaatggttt aacgcagata aaggttttgg ctttatcact
    19801 cctgatgatg gcagcaaaga cgttttcgtc catttcaccg ccatccagag caatgaattc
    19861 cgcacgctga acgaaaatca gaaagttgaa ttttctattg agcaggggca acgtggcccc
    19921 gcggcagcga acgttgttac gctctaaggt tgccgttatt actcaacatc tccatttccg
    19981 ctgtccatgt tgtcatggtt cacagtaccg cacatcggca ttcgatgtga cggagcgaaa
    20041 ccctttgggc gctaagtgta ttttttgtaa atcgacgatg atcacctttg ataacgtcgc
    20101 gctgcaaata cgcactgacc atgcgccgct ggatttcaca aaataatatt aggctccctc
    20161 gtggagcctt ttttatatct gccttatttt tcttcaacgc tgtatgtata gtaagcgata
    20221 acctgttgat tattgaatct ttcggggaga tggcttataa catttcttac ctgaccaggg
    20281 taccgggaac caacacctta ctggcgtgtt gctgtctttt aagaccagaa gaggttaaca
    20341 gtgaatattg aagagttaaa aaaacaagcc gaaacggaaa tcgccgactt tatcgcgcaa
    20401 aaaatcgccg agctgaacaa gaatacaggg aaagaagtct ctgaaattcg cttcaccgca
    20461 cgagaaaaaa tgaccgggct tgaaagttat gatgtcaaaa tcaaaataat gtgattttgt
    20521 gaacatcacc cgtgcgaggt gatgttccgc ttgttgctaa tttagtgacc aatcattggc
    20581 gcttgtggaa ttaagcgtcg gtacaattca tccggtaccg ggctttgcca tactcccgca
    20641 tacattgcgt aaccaatcac cgcaaacata atccccagaa ccagtagcgt cattaaccag
    20701 ccagacaacg caaaggcttt tttatttgcc gcaggttttt gcagtgaaaa ggtcaatgtt
    20761 gaggctaccg gacatgactc tacgcaagtc atacagccgg tacattccac tgttcgtacc
    20821 tgaattaatt tatcgaccgg gatccgtgat gggcaatttt ttgcgcattt gccacagtcg
    20881 atacaacttt cggcattgcg acgaatctta aacggcgaca atagcgaaac cacgcccatc
    20941 agcgcgccat atgggcaaag ataacgacac caggcatggc gaataaacag gctggcaatc
    21001 agcaaaacgg tcacgctgat taatgtcgcg gtccccatat gacgaaagaa atcgagcatt
    21061 ttaacgtcca tcaccacgct gtagggcgac aacataaaat agtgaatcgc ctgagcgggc
    21121 atcaataatg cgatatagag aaaaaaactc aacagcaaat acttcacgcc gcgcagagga
    21181 atatccagcc agcggggaag gacacattgc cgaccaaaca gtttgttacc gagatcgccg
    21241 attaattcag aaagcgtacc aaccgggcat aaccatgagc aaaaggcctt tttgagtaat
    21301 agactgatga cgataaaagc gaccaataac agcatcgcgg cggcgtggac ggacggtaac
    21361 tgacctgttg taaggctata tttcagattc atcagaccgg caatcggtag ccagccttcg
    21421 atacctcccg gtctggcgac aaatgtcgtg ctacttgccg tttcgtaata gcgcacccaa
    21481 taccagaacg tgatggcaat ataaatattc attgccaaca gtaataattg cgtcgcttta
    21541 cgccaggtcg tggcattgcg ccagtcattc cacggtaatt tgccgcccgt cgtgcctggc
    21601 cgccgctgcc agcgggttct ttttttctct gccatgattt tgccagtccg ttaagttgca
    21661 taccaaatgc cactattcta gttgttctta actggctgat attgattcaa atcgcgttca
    21721 ggtctttctt attcaaccat gcttccagat cggcaacact gcgtgtaatt tcttcgtgtg
    21781 gaaggggggc agataatggc tgctgctcca gttgtgcgca tagctggctg gcgatacgca
    21841 ttcccaaact cgagcaactg ctttttagct gatgcgcggc acgctttatt ttctcgctat
    21901 cctgactggc gcgggcaatg tcgatttcat cgagaagcgg cagggcatgt tgtgtaaata
    21961 ataccagcca ttcgtggatc ttctccgtcc ccattaactg agcatcttca ttgagttgcg
    22021 atacatccag cgattgatcg ttattgactt gcagttggaa atagtgcgcc agtaactgac
    22081 cgagcacttc acgcggcacc ggtttaggga taatcccgcg gaataatgaa ctggtacgct
    22141 ggcgcagcgt ttcgtcaatg acatgggcgc taaagccaat caaaaccagc gacggatatt
    22201 gctgtgccag ttgtcgggca agcgtaatgc cgtcgacatc cggcagatca aaatccacca
    22261 gtgcggcagc aaacggttcg ctattttgca gtgtctctaa agcctgcgcg gcattgccaa
    22321 cagcaacaac ctgcgcacca ctggtgttca gcatctcaac ggtaattcgc tgggttagcg
    22381 ggttatcttc aattaacagc aaacgtaaac cgtcaagacg caccgcctga ttgactgttt
    22441 ttggcacggg tgccgtggca acacgtaacg gcaagcgtaa acaaaaacag cttccaacct
    22501 ccggcgtgct ggtggcgctc agttcgccgc ccatcgcctg ggccagacgg ctactgatag
    22561 tcagccccag cccggtgccg ccgcgtttgc cgcttacctg cacaaatggc tggaagattt
    22621 ctgccagttt cgcgggatca ataccgcagc cgctgtcttc cacttcgacc agccattgct
    22681 cgccatcagt gcgactacgc aggataatgt acccttcgtc agtaaaacgc agggcgttgc
    22741 tcaacaggtt ggttataacc tgacgaatac gtcgtggatc gcccattaac gcgcacggca
    22801 tatcatcggc aattgccgtt gccaggcgaa tcgggcgacc tttcacccgt ccgctcatta
    22861 attgcagggt actttccagc agcgggcgcg gttcaaaggg ctcatcgctg accgaaacat
    22921 tcttgccacc tgcttcgata gcggaataat cgagaatatc gttgaggatg gtcagcaacg
    22981 attcaccaga gtcagtaatt gcccgcaaat catcacgctg ggcgttaagt gcggggttat
    23041 ctgccagcag ttgagcagtg ccgagaatac cgtacagcgg tgtgcggatc tcatggctca
    23101 tcgccgccag aaacgccgat tttgcctggc tggctttttc tgcttccgcc cgtgcctgtc
    23161 ggtgttctat caccagttcc tgcaattcag ctgtacgcgc tttgacctgc gccgccagct
    23221 gttcgcggtg gcgattcagt gcatgaacat tgctgcgaaa cgcatccatc agccgcccga
    23281 tggtatccag ctcccgtacg ccagcggttt ccgggaaagg ggagtcaata tcaccgtcca
    23341 gcagccgttg cagcgcctgc gtttgttcgg caagtggacg cgtgactgag cgataaacca
    23401 cgcgccaaag gatcagaatc agtgcgcaaa gtgaaaccat ccccagcaat aacaggctgt
    23461 attgcccgcg tgcactggct ttttccagat gcgccagtcc gtgctgatta cgcagctcaa
    23521 tggtgtcgac cagctgactg acttcgctac taaactgcgc gaactgggcg atgttatttt
    23581 gtgcgagagt ttgtaggtga ttgctgattt cactgtcctg ctgatacagc gccagcaaat
    23641 cgctatattg gctaacggta gttaacgttg ttgcgacctg cgcacgaaca cccggatctt
    23701 caatgcgtat ttgccgacgt tgcagaattt tcaccgcatt attgagctgc ttttccagcg
    23761 ttggtgcatt tttctggatc tgctccagcc ccagattcat caccatttgc tgcacccgca
    23821 gagcgctaag gcgcagttca ttcatctggt taacatactc aagatcgata tcaatcagcc
    23881 gatcgagtgc actttcagca gcctgacgct gatcttgttc gatcaaatcg taaatcccgg
    23941 cctgggtcgc tccagcggaa gttgtcgcat tattcgcctg accttgcgcc aggcgtgcga
    24001 tctcatcggc ggcagcgact atctgctgac tgagttgccg ttgttgctgg cgtagttgca
    24061 gacgccgccc caccagttcc ccttgctgac gtaatgaacg ggagatctcc tgctcctgtt
    24121 gttcaatagc ggtggtatca aaaccttgtt cccgtaacgc ttgcagcaac gcattaatct
    24181 tcaggctttg tgcggtgagc attcgcccct gcgcctgcca catcttttcg ttatcggcac
    24241 tggtcaggtt ctgcgcggcg aaaagttccc aggcgctggc ttcgctcaac tggcgcgcca
    24301 tattcatggt aggaatcaat gcctgagtgt tgtctttttc cacctggctg ataaagcgca
    24361 ggttgtacca tcccaccagg gtactggtca gggttaacag cgccatcagg gcaaagccca
    24421 tccagagtct tcgggtcagg gttaaattca cggtcggtgc acttaaggtg aaaaaggttg
    24481 agtcgcaaag cggaatgcat ctagcataaa gccttattat tgatgaggct atcatgcgcg
    24541 tactgctatt tttacttctt tcccttttca tgttatcggc attttcggct gataacctgt
    24601 tgcgctggca tgatgcgcag cattacacgg tgcaagcctc tacgccgctt aaagccaaac
    24661 gcgcatggaa actgtgcgcg ctttatccca gcctgaaaga ttcatactgg ttatcgttga
    24721 actatggtat gcaggaggca gctcgccgct atggtgtgga tttaaaagtg ctggaggcag
    24781 gcggctacag ccagttggct acccagcaag cacaaatcga ccagtgtaaa cagtggggcg
    24841 cagaggccat tttgctcggt agtagcacta cgtcatttcc cgacctgcaa aagcaggtag
    24901 caaatctgcc ggtgatcgaa ctggtaaatg ctcttgatga tcccctggtg aaaagccgcg
    24961 ttggtgtacc ctggtttcag atgggctatc aacctgggcg atatttggtg caatgggcgc
    25021 acggtaaacc actgaacgtg ctgttgatgc ccggacccga taatgccggg ggcagtaaag
    25081 agatggtcga gggttttcgc gcagccattg ccggaagccc ggtgcgtatt gttgatattg
    25141 cgcttggtga taacgatatt gaaatccagc gtaacctgtt gcaggagatg ctggaacgcc
    25201 acccagaaat cgacgtcgtt gccggaacgg ccattgcggc agaggcggca atgggtgaag
    25261 ggcgtaacct gaaaacgccg cttaccgtgg tgtcgtttta tctttcacat caggtgtatc
    25321 gcgggctgaa gcggggaaga gtgattatgg ctgccagcga tcaaatggtc tggcaggggg
    25381 aactggcggt tgagcaggcc atcaggcaat tacaggggca atcggtgtct gataatgtca
    25441 gcccaccgat tttagttctg acgccgaaaa atgccgaccg tgaacatatt cgccgctcgc
    25501 tgtcaccagg gggatttcgt ccggtctatt tttatcagca cacatcagcg gctaagaaat
    25561 aaccttcacc atgttgcgtc accagtaaat ccgcgctgag tttatgacgt aaacgacgaa
    25621 ttaacacatc gacggtgcgc aggtcagggt tttccacccg acgcgcagaa agcatacgta
    25681 gcagacgttc acggctgaga atttcgcccg gattcgtcac aaatgccacc aacatttcat
    25741 actctgcgcg ggtcagttta atcggctcgc catcccgctc cagcgtatgg cgcgacacat
    25801 tcaggcaata accggcaaag cgatagcagt tgtcctgagt gtgcggttga gcttgtcgcg
    25861 cgaggtcgat tcgccagagc agatttttca cccgtactac cagttcgcgc agttccagcg
    25921 gtttggtgac gtaatcgtct gcgcccattt ccagcccaac aatacggtca atccgatcgc
    25981 tgcgtccggt aaccagaata atccccaccg ttgagcgttc tcgcagggcg cgggttaaca
    26041 tcaggccatt ttcatcgggt aagttgatat ccagcagaat taaatctacc ggctgattct
    26101 gcataatttc ccgcagcccg gcaccgctcg ctgtaacgga aacggtatac ccctcctgag
    26161 tgaagtaaga ttgtaatcgc gcctgggtaa ccggctcatc ttcaacaata acaatgtgat
    26221 gtggcatcag agggttttac tcattctgtt catatctgtt catattctgc cgtaagccgt
    26281 tcatcctgac cagtgccgct gttcatattt gctcattaag atcgcttcac taaaccataa
    26341 ttttacaggg gctattatgc ggaaactctg gaacgcgcta cgccgaccca gtgctcgttg
    26401 gtcggtactg gcgctggtcg caattgggat tgtgattggc attgcgctga ttgtattgcc
    26461 acacgttggg atcaaagtca ccagcacaac cgaattttgt gtcagttgcc acagtatgca
    26521 accggtgtat gaagaatata aacagtcggt gcatttccag aacgcctccg gcgtgcgagc
    26581 tgaatgccat gactgtcata tcccgccgga tattccaggc atggtgaagc gcaaactgga
    26641 agcgagcaat gatatctacc agacctttat tgctcactcc attgatacac ctgaaaaatt
    26701 cgaagccaaa cgcgcggaac ttgccgagcg tgaatgggcg cgaatgaaag aaaacaactc
    26761 ggcaacctgc cgctcctgcc ataactacga tgcgatggat catgcgaagc agcatcctga
    26821 agcggcacgt cagatgaagg tggcagcgaa agataatcaa tcctgcatcg actgtcataa
    26881 aggtattgcc caccagttac cggatatgag tagcggcttc cgtaagcagt tcgatgagct
    26941 gcgcgccagt gctaatgaca gtggtgacac gctgtactct attgatatta agccaattta
    27001 tgcggcgaaa ggcgataaag aagcctctgg ttctctgctg cctgcttcgg aagtgaaagt
    27061 ccttaaacgt gacggcgact ggctgcaaat tgaaattacc ggctggacgg aaagcgccgg
    27121 acgtcagcgt gtactcaccc aattcccagg taaacgcatc tttgttgcct cgattcgtgg
    27181 tgatgtgcag cagcaggtaa aaacgctgga gaaaaccacc gttgccgaca ccaataccga
    27241 gtggagcaag ttgcaggcca ctgcgtggat gaagaaaggc gacatggtga acgatatcaa
    27301 accgatctgg gcttatgcgg attcgttgta caacggcacc tgtaaccagt gccacggcgc
    27361 accggaaatc gcccactttg acgctaacgg ttggatcggc acgctcaacg gcatgattgg
    27421 ctttaccagt ctcgataaac gtgaagaacg caccttgttg aaatatctgc aaatgaatgc
    27481 gtctgacacc gcaggtaagg ctcacggcga taagaaggaa gaaaaataat gaacaataac
    27541 gatctctttc aggcatcacg tcggcgtttt ctggcacaac tcggcggctt aaccgtcgcc
    27601 gggatgctgg ggccgtcatt gttaacgccg cgccgtgcga ctgcggcgca agcggcgact
    27661 gacgctgtca tctcgaaaga gggcattctt accgggtcgc actggggggc tatccgcgcg
    27721 acggtgaagg atggtcgctt tgtggcggca aaaccgttcg aactggataa atatccgtcg
    27781 aaaatgattg ccggattgcc ggatcacgta cacaacgcgg cgcgtattcg ttatccgatg
    27841 gtacgcgtgg actggctgcg taagcgccat ctcagcgata cctcccagcg cggtgataac
    27901 cgttttgtgc gcgtgagctg ggatgaagcc ctcgacatgt tctatgaaga actggaacgc
    27961 gtgcagaaaa ctcacgggcc gagtgccttg ctgaccgcca gtggttggca atcaacgggg
    28021 atgttccata acgcttcggg gatgctggcg aaagctattg ccttgcatgg taatagcgtt
    28081 ggtacgggcg gagattactc taccggtgct gcgcaggtga tcctgccgcg cgtggttggc
    28141 tcaatggaag tgtatgaaca gcaaacctcc tggccgctgg tattgcagaa cagcaaaacc
    28201 attgtgctgt ggggctctga tttgctgaaa aaccagcaag cgaactggtg gtgcccggat
    28261 cacgatgttt atgaatatta cgcgcagcta aaagcgaaag tcgccgccgg tgaaattgag
    28321 gtcatcagca tcgatccggt tgtcacatcc acccatgagt atctggggcg cgagcatgtg
    28381 aagcacattg cggttaaccc gcaaactgat gtgccgctgc aactggcgct ggcgtatacg
    28441 ctgtacagtg aaaacctgta cgacaaaaac ttcctcgcta actactgtgt gggttttgag
    28501 cagttcctgc cgtacctgct gggtgagaaa gacggtcagc cgaaagatgc cgcatgggct
    28561 gaaaaactga ccggcattga tgccgaaagc attcgtgggc tggcgcggca gatggcggcg
    28621 aacagaacgc aaattattgc tggctggtgc gtgcagcgta tgcagcacgg tgaacagtgg
    28681 gcgtggatga ttgtggttct ggcggcgatg ctggggcaaa ttggcctgcc aggtggtggc
    28741 tttggttttg gctggcacta taacggcgca ggcacgccgg ggcgtaaagg cgttattctg
    28801 agtggtttct ccggctctac gtcgattccg cctgttcacg acaacagtga ttacaaaggc
    28861 tacagcagca ccattccgat tgcccgtttt atcgatgcga tcctcgaacc ggggaaagtg
    28921 atcaactgga acggtaaatc ggtaaaactg ccgccgctga aaatgtgtat ttttgccgga
    28981 actaacccat tccatcgcca tcagcagatc aaccgcatta ttgaaggctg gcgcaagctg
    29041 gaaacggtta tcgccataga taaccagtgg acctcaacct gccgctttgc cgatatcgtg
    29101 ctgcctgcga ccacgcagtt tgagcgtaac gatctcgacc agtacggcaa ccactccaac
    29161 cgtggcatta tcgccatgaa acaggtggtg ccgccgcagt tcgaggcgcg caacgacttt
    29221 gatattttcc gcgagctgtg ccgtcgcttt aatcgcgaag aagcctttac cgaagggctg
    29281 gacgaaatgg gctggctgaa acgcatctgg caggaaggtg tacaacaagg caaaggacgc
    29341 ggcgttcatc tgccagcgtt tgatgacttc tggaataaca aagagtacgt cgagtttgac
    29401 catccgcaga tgtttgttcg ccaccaggca ttccgcgaag atccggatct cgaaccgctg
    29461 ggcacgccga gtggcctgat tgagatctac tcgaaaacca tcgccgatat gaactacgac
    29521 gattgtcagg ggcatccgat gtggtttgag aaaatcgaac gctcccacgg tgggcccggc
    29581 tcgcagaagt atccgttgca tctgcaatct gtgcatccgg atttccgact tcactcgcag
    29641 ttatgtgagt cggaaactct gcgtcagcaa tatacggtag cgggtaaaga gccagtgttc
    29701 attaacccgc aggatgccag cgcgcgcggt attcgtaacg gtgatgtggt acgcgtcttt
    29761 aacgctcgcg gtcaggtgtt ggcaggggca gtagtttctg accgctatgc acccggcgtg
    29821 gcacgaattc acgaaggggc atggtacgat ccagataaag gcggcgagcc tggtgcgctg
    29881 tgcaaatacg gtaaccccaa cgtgctgacc atcgacatcg gtacttcgca gctggcgcag
    29941 gcgaccagtg cgcacactac gctggtggaa attgagaagt acaatggagc agtggagcag
    30001 gtgacggcgt ttaacggccc tgtggagatg gtggcgcagt gcgaatatgt tcccgcgtcg
    30061 caggtgaaat catgaccacg ctgacagcac aacagattgc ctgtgtttac gcctggctgg
    30121 cgcagttgtt ctcccgtgaa ctggacgatg aacaactgac gcaaatcgcc agtgcgcaga
    30181 tggctgaatg gttttcgttg ctgaaaagcg aaccgccgct ggctgcggcg gtgaatgagc
    30241 tggaaaactg tattgccacg ctgacagtac gtgacgatgc ccgtctggaa ctggccgctg
    30301 acttttgcgg cctgtttctg atgaccgaca aacaagcggc gctgccgtat gcatcggcct
    30361 acaaacagga cgagcaagag attaaacgct tgttagttga ggcagggatg gaaaccagcg
    30421 gcaatttcaa cgaaccggca gatcatctgg cgatctatct cgaattgctc agccatctgc
    30481 atttttcgct gggagagggg accgttcctg cgcgaagaat cgacagtttg cggcaaaaaa
    30541 cactgacggc gctgtggcaa tggttaccag agtttgttgt gcgttgtcgt cagtatgaca
    30601 gctttggttt ttacgcggca ctaagccagt tattgctggt gttagtggag agcgaccacc
    30661 aaaacagata acgtcgtttg tgcgcctgaa aagacgcgtt tagcgtcgca tcaggcatta
    30721 tggggcagtt gccggatgcg tcatcacatc cggcaaacac acatcacgga tgagctacaa
    30781 accgggaaag tcgctggcgc agcaggcggt tttcctgctt caggtgcgca atatcatcca
    30841 ttaacgtcag cgccaccgcg atcccaggcc agtccagagc cagttcatga cgcaggcgta
    30901 ctgcgcgttg caccacgatg gcggcatggt cgtcaaatac ccaggttgtt tcctggatct
    30961 cacgcggttc aaccaccccc aaaccgacaa tttcattcaa ctcctcttca gagatgccgg
    31021 tatgcaggca aaattcggta atagtaaaag tcaccgtaac attagccatt atgctttccc
    31081 ccaatcttta cgtggatcaa aagacgactg ggcgtctgcc agttgctgcc acagcgcggc
    31141 agtgttttca tccggtttcg gcggcatcac gattttcagt accgcataca gatcgccggt
    31201 ctgttttttg ctcaccagac ctttgccttt aacgcgcaat cgttgcccgg cctggctgcc
    31261 tggcgggata gtcagcaaaa tgctttcttt cagtgttgga acggtgactt tagcacccag
    31321 cgccgcttcc cacgggctaa ccggcaccac aatttccaga tcctggccga caatatcgaa
    31381 cagcggatgt ggcgcaatat gaatcaccag ccacaaatcg ccatttggac caccgttttc
    31441 gcccggcgtc ccctggcctt tcagacggat gcgttgacca ttgccgacgc ccgccgggat
    31501 cttcacattc agcgttttcg ggatttcctg ttcgatcatg ccaaaggcgt tataaaccgg
    31561 caggttatag ctgatggtac gcttatgctc agtcagcgtt tcttcgagga ataccgccac
    31621 ttcgatttca atatcgtggc cgcgtgcggc ggggcgttga cggctctggc gggcatgctg
    31681 accgaaaatt gacgagaaga tatcgtcaaa atcttcggca ttaaaactct gaccgtcgcc
    31741 atgatggaac tgacggttaa attgcggatc gttgcgatgt tgccacatct gatcatactc
    31801 agcgcgacgt tgttcatcac ttaacacttc ccaggcttca gcgacctctt tgaagcgggc
    31861 ttcggcatcc ggttctttgc tgacatcagg atggtattta cgggccagtc gacgataggc
    31921 ggtcttgatt gtcttgagat cgtccgtcgg tttcacgccc atgatggcgt aataattctt
    31981 taattccata gcgttatctc gcgtaaatca acacaaattg aaggaacccc tgtaaggtaa
    32041 ctcctataag tgtagggtaa tcctcaaaat ttcatatgcc aacacagaat atgttattga
    32101 aatcatcgcg gagaggaggt cgccatcaag atgggttgct gaacatattt taaacaggtg
    32161 aaaaagggtg agcgattttt gatagttgaa ccaggcactt taagtttaac tggggcgtca
    32221 ttatttatta aattttatag acgctatata tgggtagtaa tatacatgga attagttgca
    32281 ctgcaaataa ttatttgaaa caggcctgga acgatataaa aaatgagtac gaaaaaaatc
    32341 aaacatattc aatcacgctt tttgaaaaca cactggtgtg ttttatgcgg ttatacaatg
    32401 aactcagacg taaagtaaat gaagaggata ctccatgtct ggaatgtgaa tcactagaaa
    32461 aagaatttga ggaaatgcag aatgataatg atctatcatt atttatgaga acattgcgta
    32521 ctaatgatac acaaatttat tcaggggttt caggaggtat tacatat
//
LOCUS       DAZFQD010000043        32443 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.043, whole genome shotgun sequence.
ACCESSION   DAZFQD010000043 DAZFQD010000000
VERSION     DAZFQD010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32443
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.043"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            91..1380
                     /gene="hpaG"
                     /locus_tag="ACHKTW_002097"
     CDS             91..1380
                     /gene="hpaG"
                     /locus_tag="ACHKTW_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008704 -
                     5-carboxymethyl-2-hydroxymuconate delta-isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0018800 -
                     5-oxopent-3-ene-1,2,5-tricarboxylate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:1901023 - 4-hydroxyphenylacetate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate degradation bifunctional
                     isomerase/decarboxylase"
                     /protein_id="HFO6463122.1"
                     /translation="MKGTIFAVALNHRSQLDAWQEAFQQSPYKAPPKTAVWFIKPRNT
                     VIGCGEPIPFPQGEKVLSGATVALIVGKTATKIREEDAAEYIAGYALANDVSLPEESF
                     YRPAIKAKCRDGFCPIGETVALSNVDNLTIYTEINGRPADHWNTADLQRNAAQLLSAL
                     SEFATLNPGDAILLGTPQARVEIQPGDRVRVLAEGFPPLENPVVDEREVTTRKSFPTQ
                     PHPHGTLFALGLNYADHASELEFKPPEEPLVFLKAPNTLTGDNQTSVRPNNIEYMHYE
                     AELVVVIGKQARNVSEADAMDYVAGYTVCNDYAIRDYLENYYRPNLRVKSRDGLTPML
                     STIVPKEAIPDPHNLTLRTFVNGELRQQGTTADLIFSVPFLIAYLSEFMTLNPGDMIA
                     TGTPKGLSDVVPGDEVVVEVEGVGRLVNRIVSEETAK"
     gene            1377..2843
                     /gene="hpaE"
                     /locus_tag="ACHKTW_002098"
     CDS             1377..2843
                     /gene="hpaE"
                     /locus_tag="ACHKTW_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018480 -
                     5-carboxymethyl-2-hydroxymuconic-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:1901023 - 4-hydroxyphenylacetate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HFO6463123.1"
                     /translation="MKKVNHWINGKNVAGNDYFQTTNPATGEVLADVASGGEAEINQA
                     VAAAKEAFPKWANLPMKERARLMRRLGDLIDQNVPEIAAMETADTGLPIHQTKNVLIP
                     RASHNFEFFAEVCQQMNGKTYPVDDKMLNYTLVQPVGVCALVSPWNVPFMTATWKVAP
                     CLALGNTAVLKMSELSPLTADRLGELALEAGIPAGVLNVVQGYGATAGDALVRHHDVR
                     AVSFTGGTATGRNIMKNAGLKKYSMELGGKSPVLIFEDADIERALDAALFTIFSINGE
                     RCTAGSRIFIQQSIYPEFVKRFAERANRLRVGDPNDPNTQVGALISQQHWEKVSGYIR
                     LGIEEGATLLAGGPDKPSDLPAHLKGGNFLRPTVLADVDNRMRVAQEEIFGPVACLLP
                     FKDEAEGLRLANDVEYGLASYIWTQDVSKVLRLARGIEAGMVFVNTQNVRDLRQPFGG
                     VKASGTGREGGEYSFEVFAEMKNVCISMGDHPIPKWGV"
     gene            2845..3696
                     /gene="hpaD"
                     /locus_tag="ACHKTW_002099"
     CDS             2845..3696
                     /gene="hpaD"
                     /locus_tag="ACHKTW_002099"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0008687 - 3,4-dihydroxyphenylacetate
                     2,3-dioxygenase activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HFO6463124.1"
                     /translation="MGKLALAAKITHVPSMYLSELPGKNHGCRQGAIDGHKEISKRCR
                     EMGVDTIIVFDTHWLVNSAYHINCADHFEGVYTSNELPHFIRDMTYNYEGNPELGQLI
                     ADEALKLGVRAKAHNIPSLKLEYGTLVPMRYMNEDKHFKVVSISAFCTVHDFADSRKL
                     GEAILKAIEQYDGTVAVLASGSLSHRFIDDQRAEEGMNSYTREFDRQMDERVVKLWRE
                     GQFKEFCNMLPEYADYCYGEGNMHDTVMLLGMLGWDKYDGKVEFITELFPSSGTGQVN
                     AVFPLPA"
     gene            3706..4086
                     /locus_tag="ACHKTW_002100"
     CDS             3706..4086
                     /locus_tag="ACHKTW_002100"
                     /EC_number="5.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HFO6463125.1"
                     /translation="MPHFIVECSDNIREEADLPGLFAKVNPTLAATGIFPLAGIRSRV
                     HWVDTWQMADGQRDYAFVHMTLKIGAGRSLESRQQAGEMLFELIKTHFAALMESRLLA
                     LSFEIEELHPTLNFKQNNVHALFK"
     gene            4197..5000
                     /gene="hpaH"
                     /locus_tag="ACHKTW_002101"
     CDS             4197..5000
                     /gene="hpaH"
                     /locus_tag="ACHKTW_002101"
                     /EC_number="4.2.1.163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018817 - 2-oxo-hept-3-ene-1,7-dioate
                     hydratase activity [Evidence IEA];
                     GO_process: GO:0009056 - catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hept-4-ene-1,7-dioate hydratase"
                     /protein_id="HFO6463126.1"
                     /translation="MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQR
                     EWVRLKIAEGRTLKGHKIGLTSKAMQASSQISEPDYGALLDDMFFHDGSDIPTDRFIV
                     PRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFD
                     TISDNAANAGVILGGRPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANGVAWL
                     ANKLAPYDVQLEAGQIILGGSFTRPVPARKGDTFHVDYGNMGSISCRFV"
     gene            5011..5799
                     /gene="hpaI"
                     /locus_tag="ACHKTW_002102"
     CDS             5011..5799
                     /gene="hpaI"
                     /locus_tag="ACHKTW_002102"
                     /EC_number="4.1.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018802 -
                     2,4-dihydroxyhept-2-ene-1,7-dioate aldolase activity
                     [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxoheptanedioate aldolase"
                     /protein_id="HFO6463127.1"
                     /translation="MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGAGFDWLLIDGE
                     HAPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIKQLLDVGTQTLLVPMVQNADEAR
                     EAVRATRYPPAGIRGVGSALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQI
                     LDVEGVDGVFIGPADLSADMGYAGNPQHPEVQAAIEQAIVQIRESGKAPGILIANEQL
                     AKRYLELGALFVAVGVDTTLLARAAEALAARFGAQATAVKPGVY"
     gene            5922..7298
                     /gene="hpaX"
                     /locus_tag="ACHKTW_002103"
     CDS             5922..7298
                     /gene="hpaX"
                     /locus_tag="ACHKTW_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1901241 - 4-hydroxyphenylacetate
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1900754 - 4-hydroxyphenylacetate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate permease"
                     /protein_id="HFO6463128.1"
                     /translation="MSDTSPAIPESIDPANQHKALTAGQQAVIKKLFRRLIVFLFVLF
                     IFSFLDRINIGFAGLTMGRDLGLSATMFGLATTLFYAAYVIFGIPSNIMLSIVGARRW
                     IATIMVLWGIASTATMFATGPTSLYVLRILVGITEAGFLPGILLYLTFWFPAYFRARA
                     NALFMVAMPVTTALGSLVSGYILSLDGVMALKGWQWLFLLEGFPSVLLGVMVWFWLDD
                     SPDKAKWLTKEDKKCLQEMMDNDRLTLVQPEGAISHHAMQQRSMWREIFTPVVMMYTL
                     AYFCLTNTLSAISIWTPQILQSFNQGSSNITIGLLAAVPQICTILGMIYWSRHSDRRQ
                     ERRHHTALPYLFAAAGWLLASATDHNMIQMLGIIMASTGSFSAMAIFWTTPDQSISLR
                     ARAIGIAVINATGNIGSALSPFMIGWLKDLTGSFNSGLWFVATLLVIGAGIIWAIPMQ
                     SSRPRATP"
     gene            7308..8198
                     /gene="hpaA"
                     /locus_tag="ACHKTW_002104"
     CDS             7308..8198
                     /gene="hpaA"
                     /locus_tag="ACHKTW_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate catabolism regulatory
                     protein HpaA"
                     /protein_id="HFO6463129.1"
                     /translation="MCDRQIANIDISKEYDESLGTDDVHYQSFARMAAFFGRHMLPHR
                     HEQYFQMHFLNSGQIELQLDDHRYSVEAPLFVLTPPSVPHAFITESDADGHVLTVRED
                     LIWPLLEVLYPGTRETFGLPGICLSLADKPDELAALEHYWQLIERESVEQLPGREHTL
                     TLLAQAVFTLLLRNAKLDDHAASGMRGELKLFQRFNMLIESHFHQHWTVPDYANELHI
                     TESRLTDICRRFANRPPKRLIFDRQLREAKRLLLFSDNAVNNIAWQLGFKDPAYFARF
                     FNRLVGCSPSAYRAKKVPVT"
     gene            8449..10011
                     /gene="hpaB"
                     /locus_tag="ACHKTW_002105"
     CDS             8449..10011
                     /gene="hpaB"
                     /locus_tag="ACHKTW_002105"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016712 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, reduced flavin or flavoprotein as one
                     donor, and incorporation of one atom of oxygen [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
                     /protein_id="HFO6463130.1"
                     /translation="MKPEDFRASTQRPFTGEEYLKSLQDGREIYIYGERVKDVTTHPA
                     FRNAAASVAQLYDALHKPEMQDSLCWNTDTGSGGYTHKFFRVAKSADDLRQQRDAIAE
                     WSRLSYGWMGRTPDYKAAFGCALGANPGFYGQFEQNARNWYTRIQETGLYFNHAIVNP
                     PIDRHLPTDKVKDVYIKLEKETDAGIIVSGAKVVATNSALTHYNMIGFGSAQVMGENP
                     DFALMFVAPMDADGVKLISRASYEMVAGATGSPYDYPLSSRFDENDAILVMDNVLIPW
                     ENVLIYRDFDRCRRWTMEGGFARMYPLQACVRLAVKLDFITALLKKSLECTGTLEFRG
                     VQADLGEVVAWRNTFWALSDSMCSEATPWVNGAYLPDHAALQTYRVLAPMAYAKIKNI
                     IERNVTSGLIYLPSSARDLNNPQIDQYLAKYVRGSNGMDHVQRIKILKLMWDAIGSEF
                     GGRHELYEINYSGSQDEIRLQCLRQAQSSGSMDKMMAMVDRCLSEYDQNGWTVPHLHN
                     NDDINMLDKLLK"
     gene            10029..10541
                     /locus_tag="ACHKTW_002106"
     CDS             10029..10541
                     /locus_tag="ACHKTW_002106"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase reductase
                     subunit"
                     /protein_id="HFO6463131.1"
                     /translation="MQLDEQRLRFRDAMASLSAAVNIITTEGDAGQCGITATAVCSVT
                     DTPPSLMVCINANSAMNPVFQGNGKLCVNVLNHEQELMARHFAGMTGMAMEERFSLSC
                     WQKGPLAQPVLKGSLASLEGEIRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFH
                     PVMLEMEAAI"
     gene            10934..13084
                     /gene="btsT"
                     /locus_tag="ACHKTW_002107"
     CDS             10934..13084
                     /gene="btsT"
                     /locus_tag="ACHKTW_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HFO6463132.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLNVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            13134..13337
                     /gene="yjiX"
                     /locus_tag="ACHKTW_002108"
     CDS             13134..13337
                     /gene="yjiX"
                     /locus_tag="ACHKTW_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFO6463133.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            13348..14304
                     /gene="yjiA"
                     /locus_tag="ACHKTW_002109"
     CDS             13348..14304
                     /gene="yjiA"
                     /locus_tag="ACHKTW_002109"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HFO6463134.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQRYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDETPHSTMVFIGIQLPEDEIRAAFAGLKK"
     gene            complement(14382..16922)
                     /locus_tag="ACHKTW_002110"
     CDS             complement(14382..16922)
                     /locus_tag="ACHKTW_002110"
                     /EC_number="3.5.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008953 - penicillin amidase activity
                     [Evidence IEA];
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin G acylase"
                     /protein_id="HFO6463135.1"
                     /translation="MKNRNRMIVNCVTASLMYYWSLPALAEQSSSEIKIVRDEYGMPH
                     IYANDTWHLFYGYGYVVAQDRLFQMEMARRSTQGTVAEVLGKDFVKFDKDIRRNYWPD
                     AIRAQIAALSPEDMSILQGYADGMNAWIDKVNTNPETLLPKQFNTFGFTPKRWEPFDV
                     AMIFVGTMANRFSDSTSEIDNLALLTALKDKYGVSQGMAVFNQLKWLVNPSAPTTIAV
                     QESNYPLKFNQQNSQTAALLPRYDLPAPMLDRPAKGADGALLALTAGKNRETIAAQFA
                     QGGANGLAGYPTTSNMWVIGKSKAQDAKAIMVNGPQFGWYAPAYTYGIGLHGAGYDVT
                     GNTPFAYPGLVFGHNGVISWGSTAGFGDDVDIFAERLSAEKPGYYLHNGKWVKMLSRE
                     ETITVKNGQAETFTVWRTVHGNILQTDQTTQTAYAKSRAWDGKEVASLLAWTHQMKAK
                     NWQEWTQQAAKQALTINWYYADVNGNIGYVHTGAYPDRQSGHDPRLPVPGTGKWDWKG
                     LLPFEMNPKVYNPQSGYIANWNNSPQKDYPASDLFAFLWGGADRVTEIDRLLEQKPRL
                     TADQAWDVIRQTSRQDLNLRLFLPTLQAATSGLTQSDPRRQLVETLTRWDGINLLNDD
                     GKTWQQPGSAILNVWLTSMLKRTVVAAVPMPFDKWYSASGYETTQDGPTGSLNISVGA
                     KILYEAVQGDKSPIPQAVDLFAGKPQQEVVLAALEDTWETLSKRYGNNVSNWKTPAMA
                     LTFRANNFFGVPQAAAEETRHQAEYQNRGTENDMIVFSPTTSDRPVLAWDVVAPGQSG
                     FIAPDGTVDKHYEDQLKMYENFGRKSLWLTKQDVEAHKESQEVLHVQR"
     gene            complement(17274..17438)
                     /gene="yjiS"
                     /locus_tag="ACHKTW_002111"
     CDS             complement(17274..17438)
                     /gene="yjiS"
                     /locus_tag="ACHKTW_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008786105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1127 domain-containing protein"
                     /protein_id="HFO6463136.1"
                     /translation="MEFHENKAKTPFIRLVQLWHAVRRWRRQMQTRRVLQQMSDERLR
                     DIGLRREDVE"
     gene            17615..19027
                     /gene="yjiR"
                     /locus_tag="ACHKTW_002112"
     CDS             17615..19027
                     /gene="yjiR"
                     /locus_tag="ACHKTW_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFO6463137.1"
                     /translation="MTRYQHLATLLAERIEQGLYRHGEKLPSVRSLSQEHGVSISTVQ
                     QAYQTLETMRLITPQPRSGYFVAQRKAQPPVPPMTRPVQRPVEITQWDQVLDMLEAHS
                     DNSIVPLSKSTPDVETPSLKPLWRELSRVVQHNLQTVLGYDLLAGQRVLREQIARLML
                     DSGSVVTADDIIITSGCHNSMSLALMAVCKPGDIVAVESPCYYGSMQMLRGMGVKVIE
                     IPTDPETGISVEALELALEQWPIKGIILVPNCNNPLGFIMPDARKRAVLSLAQRHDIV
                     IFEDDVYGELATEYPRPRTIHSWDIDGRVLLCSSFSKSIAPGLRVGWVAPGRYHDKLM
                     HMKYAISSFNVPSTQMAAATFVLEGHYHRHIRRMRQTYQRNLALYTCWIREYFPCEIC
                     ITRPKGGFLLWIELPEQVDMVCVARQLCRMNIQVAAGSIFSASGKYRNCLRINCALPL
                     SETYREALKQIGEAVYRAME"
     gene            complement(19269..20213)
                     /locus_tag="ACHKTW_002113"
     CDS             complement(19269..20213)
                     /locus_tag="ACHKTW_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001678496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HFO6463138.1"
                     /translation="MTNFTTSTPHDALFKSFLTHPDTARDFMEIHLPKDLRELCDLDS
                     LKLESASFVDEKLRALHSDILWSVKTREGDGYIYVVIEHQSREDIHMAFRLMRYSMAV
                     MQRHIEHDKRRPLPLVIPMLFYHGSRSPYPWSLCWLDEFAAPTTARKLYSAAFPLVDV
                     TVVPDDEIVQHRRVALLELIQKHIRQRDLMGLIDQLVVLLVTECANDSQITALLNYIL
                     LTGDEARFKAFISELTRRMPQHRERIMTIAERIYNDGWLLGMEKGKEEGEQRLLRLLL
                     QNGADPEWIQKITGLSTEQMQALEQPLPEIKRDPWIEY"
     gene            20680..21912
                     /gene="mdtM"
                     /locus_tag="ACHKTW_002114"
     CDS             20680..21912
                     /gene="mdtM"
                     /locus_tag="ACHKTW_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtM"
                     /protein_id="HFO6463139.1"
                     /translation="MPRFFARHAATLFFPMALILYDFAAYLSTDLIQPGIINVVRDFN
                     ADVSLAPAAVSLYLAGGMALQWLLGPLSDRIGRRPVLITGALIFTLACAATMFTTSMT
                     QFLIARAIQGTSICFIATVGYVTVQEAFGQTKGIKLMAIITSIVLIAPIIGPLSGAAL
                     MHFVHWKVLFAIIAVMGFISFVGLLLAMPETVKRGAVPFSAKSVFRDFRNVFCNRLFL
                     FGAATISLSYIPMMSWVAVSPVILIDAGGLTTSQFAWTQVPVFGAVIVANAIVARFVK
                     DPTEPRFIWRAVPIQLVGLALLIIGNLLSPHVWLWSVLGTSLYAFGIGLIFPTLFRFT
                     LFSNNLPKGTVSASLNMVILMVMSVSVEIGRWLWFNGGRLPFHLLAVVAGVIVVFTLA
                     GLLNRVRQHQAAELAEER"
     gene            21953..23233
                     /gene="yjiN"
                     /locus_tag="ACHKTW_002115"
     CDS             21953..23233
                     /gene="yjiN"
                     /locus_tag="ACHKTW_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HFO6463140.1"
                     /translation="MNKLIELRRAKMLALSLLLIAAATFVVTLFLPPNFWVSGVKAIA
                     EAAMVGALADWFAVVALFRRVPIPIISRHTAIIPRNKDRIGENLGQFVQEKFLDTQSL
                     VALIRRHEPVLLIGNWFSQPENARRVGQHLLQIMSGFLELTDDARIQRLLKRAVHRAI
                     DKVDLSGTSALMLESMTKNDRHQVLLDTLIAQLIALLQRDKSRKFIAQQIVRWLESEH
                     PLKAKILPTEWLGEHSAELVSDAVNSLLDDISRDRAHQIRHAFDRATFALIDKLKNDP
                     EMAARADAVKSYLKEDEAFNRYLSELWGDLREWLKADINSEDSRVKERIARAGQWFGE
                     TLIADDALRASLNGHLEQAAHRVAPEFSAFLTRHISDTVKSWDARDMSRQIELNIGKD
                     LQFIRVNGTLVGGCIGLILYLLSQLPALFPLSNF"
     gene            23349..24500
                     /gene="yjiM"
                     /locus_tag="ACHKTW_002116"
     CDS             23349..24500
                     /gene="yjiM"
                     /locus_tag="ACHKTW_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="double-cubane-cluster-containing anaerobic
                     reductase"
                     /protein_id="HFO6463141.1"
                     /translation="MSLITDLPAIFDQFSEARQKGFLTVMDLKERGIPLVGTYCTFMP
                     QEIPMAAGAVVVSLCSTSDETIEEAEKDLPRNLCPLIKSSYGFGKTDKCPYFYFSDLV
                     VGETTCDGKKKMYEYMAEFKPVHVMQLPNSVKDDASRALWKAEMLRLQKTVEERFGHE
                     ISEDALRDAIALKNRERRALANFYHLGQLNPPALSGSDILKVVYGATFRFDKEALINE
                     LDAMTARVRQQWEEGQRLALRPRILITGCPIGGAAEKVVRAIEENGGWVVGYENCTGA
                     KATEQCVAETGDVYDALADKYLAIGCSCVSPNDQRLQMLSQMVEEYQVDGVVDVILQA
                     CHTYAVESLAIKRHVRQQHNIPYIAIETDYSTSDVGQLSTRVAAFIEML"
     gene            24510..25277
                     /gene="yjiL"
                     /locus_tag="ACHKTW_002117"
     CDS             24510..25277
                     /gene="yjiL"
                     /locus_tag="ACHKTW_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313324.1"
                     /note="YjiL, as found in Escherichia coli, is a homolog of
                     the activator ATPases of enzymes such as lactoyl-CoA
                     dehydratase, (R)-phenyllactate dehydratase, and
                     2-hydroxyisocaproyl-CoA dehydratase. The typical substrate
                     of those enzymes is acyl-CoA with an OH at the
                     beta-position. YjiL is the putative activator for the
                     cognate protein YjiM, a putative 2-hydroxyacyl-CoA
                     dehydratase with unknown specificity, encoded by the
                     adjacent gene; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-hydroxyacyl-CoA dehydratase activator
                     YjiL"
                     /protein_id="HFO6463142.1"
                     /translation="MTYSIGIDSGSTATKGILLADGVITRRFLVPTPFRPATAITEAW
                     ETLREGLETTPFLTLTGYGRQLVDFADKQVTEISCHGLGARFLAPATRAVIDIGGQDS
                     KVIQLDDDGNLCDFLMNDKCAAGTGRFLEVISRTLGTSVEQLDSITENVTPHAITSMC
                     TVFAESEVISLRSAGVAPEAILAGVINAMARRSANFIARLSCEAPILFTGGVSHCQTF
                     ARMLESHLGMPVNTHPDAQFAGAIGAAVIGQRVRTRR"
     gene            25274..25531
                     /gene="ytiA"
                     /locus_tag="ACHKTW_002118"
     CDS             25274..25531
                     /gene="ytiA"
                     /locus_tag="ACHKTW_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HFO6463143.1"
                     /translation="MKEFLFLFHSTVGVIQTRKALQAAGMTFRVSDIPRDLRGGCGLC
                     IWLTCPPGEEIQWVIPGQTESIYCQQDGVWRCIAHYGVSPR"
     gene            25596..26456
                     /gene="yjiK"
                     /locus_tag="ACHKTW_002119"
     CDS             25596..26456
                     /gene="yjiK"
                     /locus_tag="ACHKTW_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418753.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdiA-regulated domain-containing protein"
                     /protein_id="HFO6463144.1"
                     /translation="MTKSISLSKRISVIVILFAIVAVCTFFVQSCARKSNHAASFQNY
                     HATIDGKEIAGITNNISSLTWSAQSNTLFSTINKPAAIVEMTTNGDFIRTIPLDFVKD
                     LETIEYIGDNQFVISDERDYAIYVISLTPNSEVKILKKIKIPLQDSPTNCGFEGLAYS
                     RQDHTFWFFKEKNPIEVYKVNGLLSSNELHISKDEALQRQFTLDDVSGAEFNQQKNTL
                     LVLSHESRALQEVTLVGEVIGEISLTKGSRGLSHNIKQAEGIAMDASGNIYIVSEPNR
                     FYRFTPKSSH"
     gene            26524..27702
                     /gene="yjiJ"
                     /locus_tag="ACHKTW_002120"
     CDS             26524..27702
                     /gene="yjiJ"
                     /locus_tag="ACHKTW_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO6463145.1"
                     /translation="MPSSSHPVERFSFSTALFGMLVLTLGMGLGRFLYTPMLPVMMAE
                     GAFSFSQLSWIASGNYAGYLAGSLLFSFGAFHQPSRLRPLLLVSALASGLLILAMAWL
                     PPFILVLLIRFLAGVASAGMLIFGSTLIMQHTRHPFVLAALFSGVGIGIALGNEYVLA
                     GLHFAFSSQTLWQGAGALSGMMLIALTLLMPSKKHAIAPMPLAKTEQQIMSWWLLAIL
                     YGLAGFGYIIVATYLPLMAKDAGSPLLTAHLWTLVGLSIVPGCFGWLWAAKRWGALPC
                     LTANLLVQAISVLLTLASDSPLLLIISSIGFGGTFMGTTSLVMTIARQLSVPGNLNLL
                     GFVTLIYGIGQILGPALTSMLGNGTSALAGATLCGAAALFIAALISTVQLFKLQVVTS
                     "
     gene            complement(27715..28269)
                     /gene="kptA"
                     /locus_tag="ACHKTW_002121"
     CDS             complement(27715..28269)
                     /gene="kptA"
                     /locus_tag="ACHKTW_002121"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HFO6463146.1"
                     /translation="MAKYNEKELADTSKFLSFVLRHKPEAIGIVLDREGWADIDKLIL
                     CAQKAGKRLTRALLDTVVATSDKKRFSYSSDGRCIRAVQGHSTSQVAISFAEKTPPQF
                     LYHGTASRFLDEIKKQGLIAGERHYVHLSADEATARKVGARHGSPVILTVKAQEMAKR
                     GIPFWQAENGVWLTSTVAVEFLEW"
     gene            28519..29202
                     /gene="yjiH"
                     /locus_tag="ACHKTW_002122"
     CDS             28519..29202
                     /gene="yjiH"
                     /locus_tag="ACHKTW_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HFO6463147.1"
                     /translation="MTQQGDAVAGELATEKVGIKGYLAFFLTIIFFSGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGAGGAGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDAAAGMTKELAQEGEITERDKV
                     IFAAYQTSGSAIITNYFSSGVAVFAFLGTSVIVPLAVILVFKFVGANILRVWLNFEER
                     RNPTQGAQA"
     gene            29199..29660
                     /gene="yjiG"
                     /locus_tag="ACHKTW_002123"
     CDS             29199..29660
                     /gene="yjiG"
                     /locus_tag="ACHKTW_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HFO6463148.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLPGEAATVLLAALMSMGGAVGVAASLATAGALTGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            29673..30845
                     /gene="iadA"
                     /locus_tag="ACHKTW_002124"
     CDS             29673..30845
                     /gene="iadA"
                     /locus_tag="ACHKTW_002124"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFO6463149.1"
                     /translation="MIDYTAAGFTLLQGAHLYAPEDRGICDVLVANGKIIAVASNIPS
                     DIVPDCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHPESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGGKPGVTVFHMGDSKKALQPVYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIARAVQAGIP
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYDFSISDALRPLTS
                     SVAGFLNLTGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVNGTFETA"
     gene            30910..31821
                     /gene="hypT"
                     /locus_tag="ACHKTW_002125"
                     /gene_synonym="qseD"
     CDS             30910..31821
                     /gene="hypT"
                     /locus_tag="ACHKTW_002125"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HFO6463150.1"
                     /translation="MDDCGAILHNIETKWLYDFLTLEKCRNFSQAAVSRNVSQPAFSR
                     RIRALEQAIGVELFNRQVTPLQLSEQGKIFHSQIRHLLQQLESNLAELRGGSDYAQRK
                     IKIAAAHSLSLGLLPSIISQMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLL
                     EAPFDHIRLFESQLFPVCASDEHGEALFNLAQPHFPLLNYSRNSYMGRLINRTLTRHS
                     ELSFSTFFVSSMSELLKQVALDGCGIAWLPEYAIQQEIRSGKLVVLNRDELVIPIQAY
                     AYRMNTRMNPVAERFWRELRELEIVLS"
     gene            complement(31814..32206)
                     /gene="iraD"
                     /locus_tag="ACHKTW_002126"
     CDS             complement(31814..32206)
                     /gene="iraD"
                     /locus_tag="ACHKTW_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418746.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HFO6463151.1"
                     /translation="MMRQSLQAVLPEISGNKTSSLRKSVCSDLLTLFNSPHSALPSLL
                     VSGMPEWQVHNQSDKHLQSWYCRQLRSALLFHEPRIAALQVNLKEAYSHTLAISLEIM
                     LYHDGEPLTFDLVWDNGGWCSAMLENVS"
     gene            complement(32203..32286)
                     /gene="idlP"
                     /locus_tag="ACHKTW_002127"
     CDS             complement(32203..32286)
                     /gene="idlP"
                     /locus_tag="ACHKTW_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iraD leader peptide IdlP"
                     /protein_id="HFO6463152.1"
                     /translation="MENEHQYSGARCSGQTAYVAKRQECAK"
BASE COUNT         7445 a         8566 c         8511 g         7921 t
ORIGIN      
        1 catcacttac ttttgccaaa ctacgcaatc gggaataaaa gtaatcatta acatattaat
       61 gattaaaatg atcgaatccg aggagtggta atgaaaggca ctatcttcgc cgtagcgttg
      121 aaccatcgca gccaacttga tgcatggcag gaagcgttcc agcaatcccc ctacaaagcc
      181 ccgcctaaaa ctgcggtctg gtttattaaa ccgcgcaata cggtgattgg ttgcggtgaa
      241 ccgattccct ttccacaggg tgaaaaggta ctgagcggtg cgactgttgc gctgattgtg
      301 ggaaaaacgg cgacgaaaat acgtgaagaa gatgcagcag agtacatcgc cggatatgcg
      361 ctggctaacg atgtcagcct gccggaagag agcttttacc gcccggcaat caaagcaaaa
      421 tgtcgtgatg gattctgccc cattggcgaa accgtggctc tcagcaatgt cgataatctg
      481 accatctata ccgagatcaa cgggcgtcct gccgatcact ggaataccgc cgatttacaa
      541 cgtaacgccg cgcagttgct gagcgccctg agcgaatttg ccacactgaa tccaggcgat
      601 gccattctgc tcggcacgcc acaggcgcgc gtggaaatac agccaggcga tcgcgttcgt
      661 gttctcgcag aaggtttccc gccgctggaa aatccggtag tggacgaacg tgaagtgacc
      721 acgcgcaaga gcttcccaac gcagccacac ccgcacggca cgctgtttgc cctcggcctg
      781 aactacgccg accacgccag cgaactggaa tttaagccac cggaagaacc gctggtgttc
      841 ctgaaagcgc caaataccct cactggcgat aaccagacct ccgtgcgtcc aaacaatatt
      901 gaatacatgc actatgaagc ggagctggtg gtagttattg gcaaacaggc gcgtaacgtc
      961 agcgaagccg atgccatgga ttatgtcgcg ggctacaccg tgtgtaacga ctacgccatt
     1021 cgcgactatc tggaaaacta ctaccgccct aacctgcggg tcaaaagccg cgacggactg
     1081 acgccgatgc tttcaaccat cgtgccgaaa gaggcgatcc cggacccgca taatctgacc
     1141 cttcgcacct tcgtcaacgg cgagttacgc cagcaaggca ccaccgccga tctgatcttc
     1201 agcgtgccct tcctgatcgc ctacttaagc gaatttatga ccctgaatcc gggcgacatg
     1261 atcgccaccg gcacaccaaa aggcttatct gacgtggtgc ctggcgatga agtggtggtg
     1321 gaagtagaag gcgtgggccg cctggtgaac cgaattgtga gtgaggaaac agcgaaatga
     1381 aaaaagtaaa tcattggatc aacggtaaaa atgttgcagg taacgactac ttccagacta
     1441 ccaatccggc aacgggtgaa gtgctggcgg atgtggcctc cggcggtgaa gcggagatca
     1501 atcaggcggt agcggcagcg aaagaggcgt tcccgaaatg ggccaatctg ccgatgaaag
     1561 agcgcgcgcg cctgatgcgc cgtctgggcg atctgatcga ccagaacgtg ccggagatcg
     1621 ccgcgatgga aaccgcggac acgggcctgc cgatccatca gaccaaaaat gtgttgatcc
     1681 cacgcgcttc tcacaacttt gaatttttcg cggaagtctg ccagcagatg aacggcaaga
     1741 cttatccggt cgacgacaag atgctcaact acacgctggt gcagccggta ggcgtttgtg
     1801 cactggtgtc accgtggaac gtgccgttta tgaccgccac ctggaaggtc gcgccgtgtc
     1861 tggcgctggg caataccgcg gtactgaaaa tgtcggaact ctccccgctg accgctgacc
     1921 gcctgggtga gctggcgctg gaagccggta ttccggcagg cgtgctgaac gtggtacagg
     1981 gctacggcgc aaccgcaggg gatgcgctgg ttcgtcatca tgacgtacgt gccgtgtcgt
     2041 tcaccggcgg tacggccacc gggcgcaaca tcatgaaaaa cgccgggctg aaaaaatact
     2101 ccatggaact gggcggtaaa tcgccggtgc tgatttttga agatgccgat attgaacgcg
     2161 cgctggacgc cgccctgttc accatcttct cgatcaacgg cgaacgctgc accgccggtt
     2221 cgcgcatctt tattcagcaa agcatctacc cggaattcgt taaacgcttt gccgaacgcg
     2281 ccaaccgtct gcgcgtgggc gatccgaacg atccgaatac ccaggttggc gcgcttatta
     2341 gccagcagca ctgggaaaaa gtctccggct atatccgtct cggcattgaa gaaggcgcaa
     2401 ccctgctggc gggcggcccg gataaaccgt ccgacctgcc tgcgcacctg aaaggcggca
     2461 acttcctgcg cccaaccgtg ctggcagacg ttgataaccg tatgcgagtc gcccaggaag
     2521 agattttcgg gccggtcgcc tgcctgctgc cgtttaaaga cgaagccgaa ggcttacgtc
     2581 tggcaaacga cgtggagtac ggcctcgcgt cgtacatctg gacacaggat gtcagcaaag
     2641 tgttacgcct ggcgcgtggc attgaagctg gcatggtgtt cgtcaacacc cagaacgtgc
     2701 gtgacctgcg ccagccattt ggcggcgtaa aagcctccgg caccgggcgt gaaggcggtg
     2761 agtacagctt cgaagtgttc gcggaaatga agaacgtctg catttccatg ggcgaccatc
     2821 caattccgaa atggggagtc tgatatgggt aagttagcgt tagccgccaa gatcacgcac
     2881 gtaccgtcga tgtatctctc tgaactgcca gggaaaaacc acggttgccg ccagggcgcg
     2941 atcgacgggc ataaagagat cagcaagcgt tgccgggaaa tgggcgtcga taccattatc
     3001 gttttcgata cccactggct ggtcaacagt gcttatcaca tcaactgtgc agaccatttt
     3061 gaaggcgtct acaccagcaa cgagctgccg cattttatcc gcgatatgac ctacaactac
     3121 gagggcaacc cggagttggg gcagcttatt gccgatgaag ccttaaagct cggcgtgcgg
     3181 gcaaaagcgc acaacattcc cagcctgaaa ctggaatacg gcacgctggt gccaatgcgc
     3241 tacatgaatg aagacaagca cttcaaagtg gtttccattt cagctttctg cactgttcac
     3301 gattttgccg acagccgcaa gctgggcgag gcgattctga aagcgatcga acagtacgac
     3361 ggcaccgtgg cggtccttgc cagcggttcg ttatcgcacc gctttattga cgatcagcga
     3421 gcggaagaag ggatgaacag ctacacccgc gagttcgacc gccagatgga cgagcgtgtg
     3481 gtgaagctgt ggcgcgaagg ccagttcaaa gagttctgca atatgctgcc ggagtacgcc
     3541 gactactgct acggcgaagg caatatgcac gacacggtga tgctgctggg gatgctcggc
     3601 tgggataaat acgacggcaa ggtggagttt atcaccgagc tattcccaag ctctggcacc
     3661 ggtcaggtta acgctgtttt cccgcttccc gcgtaaggac gttttatgcc gcactttatc
     3721 gttgaatgca gtgacaacat ccgcgaagaa gccgacctgc cggggttgtt cgccaaagtg
     3781 aatccgacgc tggcagccac gggtattttt ccgctggcgg gtattcgcag ccgcgtgcat
     3841 tgggtcgata cctggcagat ggccgacggg cagcgtgatt atgccttcgt gcatatgacg
     3901 ttgaaaatcg gcgcaggtcg cagcctggaa agccgtcagc aggcgggtga aatgctgttt
     3961 gaactgatta aaacgcactt cgccgccctg atggagagcc gcctgctggc gttgtcgttt
     4021 gagattgaag agctgcatcc gacgctgaat tttaaacaaa acaacgtgca cgcattgttt
     4081 aagtagcgcg cagattgccc ggtggcgctg cgcttaccgg gcctacaaaa ccccaaaccg
     4141 tacaccgtag gccggataag gcgcagccgc atccggcaat gccacaggat atcgctatgt
     4201 tcgataaaca tacccacacc ctgatcgccc agcgtctgga tcaggcagaa aaacagcgcg
     4261 aacagatccg cgcgatctcg ctggattacc cggagatcac catcgaagac gcttacgcgg
     4321 tgcagcgtga atgggttcgc ctgaaaatag ccgaaggtcg cacgctgaaa ggccacaaaa
     4381 tcggcctgac ctcgaaagcg atgcaggcca gctcacaaat cagcgagccg gattacggtg
     4441 ccctgctgga tgacatgttc ttccacgatg gcagcgatat cccgaccgat cgctttatcg
     4501 tgccgcgcat tgaagtggag ctggctttcg tgctggcaaa accgctgcgt gggccaaact
     4561 gcacgctgtt cgacgtctac aacgccacgg actacgtgat cccggcgctg gagctgatcg
     4621 acgctcgctg ccacaacatc gatccggaaa cccagcgccc gcgcaaagtg ttcgacacca
     4681 tttctgataa cgccgccaac gccggggtga tcctcggtgg tcgtcccatt aagcccgatg
     4741 agttggatct gcgttggatc tccgccctga tgtatcgcaa tggcgtgatt gaagaaaccg
     4801 gcgtcgccgc tggtgtgcta aatcatccgg caaacggcgt ggcctggctg gcgaacaaac
     4861 tcgcaccgta tgacgtacaa ctggaagccg ggcaaatcat tctcggcggt tcgttcaccc
     4921 gcccggttcc ggcgcgtaag ggcgacacct tccacgtcga ttacggcaac atgggctcca
     4981 ttagctgccg ctttgtttaa ggagagaacg atggaaaaca gttttaaagc ggcgctgaaa
     5041 gcaggccgcc cgcagatcgg attatggctg gggctgagta gcagctacag cgcagagtta
     5101 ctggccggag caggattcga ctggttgttg atcgacggtg agcacgcgcc gaataacgtg
     5161 caaaccgtgc tcacccagct acaggcgatt gcgccctacc ccagccagcc ggtagtacgt
     5221 ccgtcgtgga acgatccggt gcaaatcaaa caactgctgg acgtcggcac acaaaccttg
     5281 ctggtgccga tggtacaaaa cgctgacgaa gcccgtgaag cggtacgcgc cacccgttat
     5341 ccccccgccg gtattcgcgg tgtgggcagt gcgctggccc gcgcctcgcg ctggaatcgt
     5401 attcctgatt acctgcaaaa agccaacgat caaatgtgcg tgctggtgca gatcgaaacg
     5461 cgtgaggcaa tgaagaactt accgcagatt ctggacgtgg aaggcgtcga cggcgtgttt
     5521 atcggcccgg cggatctgag cgccgatatg ggttatgccg gtaatccgca gcacccggaa
     5581 gtacaggccg ccattgagca ggcgatcgtg cagatccgtg aatcgggcaa agcgccgggg
     5641 atcctgatcg ccaatgagca actggcaaaa cgctatctgg aactgggcgc gctgtttgtc
     5701 gccgtcggcg ttgacaccac cctgctcgcc cgcgccgctg aagcgctggc agcacggttt
     5761 ggtgcgcagg ctaccgccgt gaagcccggc gtgtattaat gccggatgcg ctgcgcttat
     5821 ccggcctaca ctcgcaccaa atcgtaggcc ggataaggcg ttacgccgca tccagcaaaa
     5881 accttgtcgt accctacaaa aatcccatta gaggaagaaa aatgagcgac acctcacctg
     5941 ccataccgga gagtatcgat ccggcgaatc agcataaagc gctgactgcc ggacaacagg
     6001 cggttattaa gaagctattt cgccgcctga tcgtctttct gttcgtgctg tttatcttct
     6061 cgttccttga tcgcatcaac atcggctttg ccggactcac gatgggacgc gacctcggtc
     6121 tgagcgccac catgtttggc ctcgctacca ccctgttcta cgccgcttat gtcatcttcg
     6181 gcattcccag caacattatg ctgagtattg tcggtgcgcg gcgctggatc gccaccatca
     6241 tggtgctttg gggcatcgcc tctactgcca ccatgtttgc cactggcccc accagcttgt
     6301 acgtactgcg tatactggtt ggcattaccg aagccggctt tctgcctggc attctgctgt
     6361 atttaacctt ctggtttcca gcctatttcc gcgcccgtgc caacgccttg tttatggttg
     6421 caatgccggt aacgacagcg ttgggatcgc tcgtttccgg ctacattttg tcgctggatg
     6481 gcgtaatggc attaaaaggc tggcagtggc tgtttttgct ggaaggcttc ccgtcggtat
     6541 tactcggcgt catggtgtgg ttctggcttg atgactcacc ggacaaagct aagtggctga
     6601 cgaaagaaga caaaaaatgc ctgcaagaga tgatggataa cgatcgtctg acgctggttc
     6661 agccagaggg agccatcagc caccacgcca tgcaacaacg cagcatgtgg cgggagatct
     6721 tcactccggt ggtgatgatg tataccctgg cgtatttctg cctgaccaac acacttagtg
     6781 cgatcagcat ctggacaccg cagatcctgc aaagctttaa tcagggcagc agtaatatca
     6841 ccatcggcct gctggccgcc gtaccgcaga tttgtaccat tctcgggatg atctactgga
     6901 gccgtcactc agatcgccgc caggaacgaa ggcatcacac cgcccttcct tatttgttcg
     6961 ctgccgcggg ttggttactg gcttcggcaa ctgatcacaa catgatccag atgctgggga
     7021 tcattatggc ttcgaccgga tcattcagcg caatggcgat tttctggaca acaccggatc
     7081 agtccatcag cctgcgggca cgagcgatcg gtattgcggt gatcaacgcc actggcaaca
     7141 ttggttcagc gttaagtccg tttatgatcg gctggttgaa agatctgacc ggcagcttta
     7201 acagtggatt gtggtttgtt gccacgctgc tggtgattgg tgcggggatt atctgggcga
     7261 ttccaatgca gtcctcccgt ccgcgagcga ccccgtaagg aacgacgatg tgtgaccgtc
     7321 agattgccaa tattgatatc agcaaagagt acgatgaaag cctgggcacg gacgatgtgc
     7381 attatcagtc gttcgcccgc atggcggcat tttttggccg ccatatgctg ccacatcgcc
     7441 acgaacagta ctttcagatg catttcctca atagcggaca gattgagcta cagcttgacg
     7501 atcatcgcta ctcggtggaa gcgcccctgt ttgtcctgac gccaccgtca gtacctcatg
     7561 cgtttattac ggagtctgac gccgacggtc atgtgttgac ggtacgggaa gatctgatct
     7621 ggcccctgct ggaagttctt tatccgggca ctcgggaaac cttcggcctg ccggggattt
     7681 gcctgtcact ggcagataaa cccgacgaac tggcggcgct ggaacactat tggcaactga
     7741 tagagcggga atcggtggaa caactgcctg gacgggaaca caccctgacg ttactggcac
     7801 aggcagtgtt caccctactg ctgcgtaacg caaaactcga cgaccatgcc gccagcggga
     7861 tgcgcggaga attaaaactg ttccagcgtt ttaatatgct gattgaaagc cattttcatc
     7921 agcactggac agtaccggat tacgctaacg aactgcatat caccgaatca cgactcacgg
     7981 acatctgccg tcgctttgcc aaccgtccgc caaaacggtt gattttcgac aggcagctgc
     8041 gagaagccaa gcggctgctg ctgttttctg ataacgccgt gaacaatatt gcctggcaac
     8101 tcggttttaa ggatccggct tattttgcgc gcttttttaa tcgcttagtc ggttgctcgc
     8161 ccagtgctta tcgcgccaaa aaagtacctg tgacgtgaaa atcccttagc cttaacggga
     8221 aaccaggcac cacctgctat tcccctttct ccgcctggag caaggggatt tagtaacgtc
     8281 aaaaatcgct aaaagcgacc tcgatcacaa aaccgtaccc gacctgaaaa gtaccagcca
     8341 ttgtcccaaa agtctcttcc cgtaatcgcc cctgcaagct tcaattgaat aacaaaaaca
     8401 acacacaaaa ttaacaattg tattccgatt aatactgtag aggtcgacat gaaaccagaa
     8461 gatttccgcg ccagtaccca acgtccgttc accggggaag agtatctgaa aagcctgcag
     8521 gatggtcgcg agatctatat ctatggcgag cgagtgaaag acgtcactac tcatccggca
     8581 tttcgtaatg cggcagcgtc tgttgcccaa ctgtacgacg cgctgcacaa accggagatg
     8641 caggactctc tgtgctggaa caccgacacc ggcagcggcg gctataccca taaattcttc
     8701 cgcgtggcga aaagtgccga cgacctgcgc cagcaacgcg acgccatcgc tgagtggtca
     8761 cgcctgagct atggctggat gggccgtacc ccagactaca aagctgcttt cggttgcgca
     8821 ctgggcgcga atccgggctt ttacggtcag ttcgagcaga acgcccgtaa ctggtacacc
     8881 cgtattcagg aaactggcct ctactttaac cacgcgattg ttaacccacc gatcgatcgt
     8941 catttgccga ccgataaagt aaaagacgtt tacatcaagc tggaaaaaga gactgacgcc
     9001 gggattatcg tcagcggtgc gaaagtggtt gccaccaact cggcgctgac tcactacaac
     9061 atgattggct tcggctcggc acaagtgatg ggcgaaaacc cggacttcgc actgatgttc
     9121 gttgcgccaa tggatgccga tggcgtgaaa ttaatctccc gcgcctctta tgagatggtc
     9181 gcgggtgcta ccggctcgcc atacgactac ccgctctcca gccgcttcga tgagaacgat
     9241 gcgattctgg tgatggataa cgtgctgatc ccatgggaaa acgtactgat ctaccgcgat
     9301 ttcgatcgct gccgtcgctg gacaatggaa ggcggttttg cccgtatgta tccgctgcaa
     9361 gcctgtgtgc gcctggcagt gaaattagac ttcattacag cactgctgaa aaaatctctc
     9421 gaatgtaccg gcaccctgga gttccgtggt gtacaggccg atctcggtga agtggtggcg
     9481 tggcgcaaca ccttctgggc attgagtgac tcgatgtgtt ctgaagcgac gccgtgggtc
     9541 aacggggctt atttaccgga tcatgccgca ctgcaaacct atcgcgtact ggcaccaatg
     9601 gcctacgcga agatcaaaaa cattatcgaa cgcaacgtta ccagtggcct gatctatctc
     9661 ccttccagtg cccgtgacct gaacaatccg cagatcgacc agtatctggc gaagtatgtg
     9721 cgcggttcga atggtatgga tcacgtccag cgcatcaaga tcctcaaact gatgtgggac
     9781 gccattggca gcgagtttgg tggtcgccac gaactgtatg aaatcaacta ctccggtagc
     9841 caggatgaga ttcgcctaca gtgtctgcgc caggcacaaa gctccggcag tatggacaag
     9901 atgatggcga tggttgatcg ctgcctgtcg gaatacgacc agaacggctg gactgtgccg
     9961 cacttgcaca acaacgacga tatcaacatg ctggataagc tgctgaaata acgcagcagg
    10021 aggttaagat gcaattagat gaacagcgcc tgcgctttcg tgacgcgatg gccagcctgt
    10081 cggcagcggt aaatattatc accaccgagg gcgacgccgg acaatgcggg attacggcaa
    10141 cggccgtctg ctcggtcacg gatacaccac catcgctgat ggtgtgcatt aacgccaaca
    10201 gtgcgatgaa cccggttttt cagggcaacg gtaagttgtg cgtcaacgtc ctcaaccatg
    10261 agcaggaact gatggcacgc cacttcgcgg gcatgacagg catggcgatg gaagagcgtt
    10321 ttagcctctc atgctggcaa aaaggtccgc tggcgcagcc ggtgctgaaa ggttcgctgg
    10381 ccagtcttga aggtgagatc cgcgatgtgc aggcaattgg cacacatctg gtgtatctgg
    10441 tggagattaa aaacatcatc ctcagtgcag aaggtcacgg acttatctac tttaaacgcc
    10501 gtttccatcc ggtgatgctg gaaatggaag ctgcgattta agctccagcc ccttcacgca
    10561 ttcgtcgaag gggcattttt attgtgatct caatcacata tccacccgat gaataattct
    10621 tgcccaaccg ctcaccgcca ttcctgaccg cttacgtccc taaaataacc actcagttat
    10681 ttaccttact ttacgcgcgc gtaactctgg caacatcact gcaggatagc ggtcaattta
    10741 cctcctcaaa cgcaacgcaa acctagaacg gcttcggcct atgattaaca aattactcta
    10801 tgaattttgg tttgcaggaa ggcagcaagt gagtgaatcc ccgggagctt acaacagtaa
    10861 gtgacagggg tgaacgaacg caactgccgc acctgtaagc taaaagacga cgagtaaaat
    10921 gccaggtttt actatggata cgaaaaaact attcaagcac ataccctggg tgattctcgg
    10981 aatcatcggt gcattctgcc tcgcggtagt tgcattacgt cggggggagc acgtcagcgc
    11041 cctgtggatc gtggtcgcct ctgtgtcggt gtatctggtg gcgtatcgct actacagtct
    11101 gtacatcgcc cagaaggtga tgaaactcga ccccacgcgc gcgacgcctg cggttattaa
    11161 caacgacggt ctgaactacg ttccgaccaa ccgttacgtg ttgtttggtc accacttcgc
    11221 cgctatcgcc ggtgctggtc cgctggtcgg tccggttctc gccgcacaga tgggctacct
    11281 gcctggcacg ctgtggctgc tggcgggggt agtactggcc ggtgcggttc aggactttat
    11341 ggtgctgttt atctcctctc gccgtaacgg cgcatctctc ggtgagatga taaaagaaga
    11401 gatggggcca gttccgggga ctatcgcgct gtttggctgt ttcttaatca tgatcatcat
    11461 cctcgccgtt ctggcgctga ttgtggttaa agccttggcc gaaagtccgt ggggtgtctt
    11521 caccgtttgc tcaaccgtac cgattgcgct gtttatgggt atctacatgc gctttatccg
    11581 tccggggcgt gtgggtgaag tctctgtcat tggtatcgtg ctgctggttg cctctatcta
    11641 cttcggtggc gtgattgctc acgatccgta ctggggtccg gcactgacct ttaaagacac
    11701 caccattacc ttcgcgctga ttggctatgc gtttgtttcc gcactgctgc cggtgtggct
    11761 gatcctcgca ccgcgcgact atctggcaac cttcctgaaa atcggcgtta tcgtcggcct
    11821 ggcgctgggt atcgtggtgc tgaacccgga actgaaaatg cctgcgatga cccagtacat
    11881 tgacggtact ggcccgctgt ggaaaggcgc tctgttcccg ttcctgttca tcaccatcgc
    11941 ctgtggtgcg gtatctggct tccacgcgct gatctcttcc ggtacgacgc caaaactgct
    12001 ggctaacgaa accgacgcgc gtttcattgg ctacggcgca atgctgatgg agtccttcgt
    12061 ggcgattatg gcgctggttg cagcgtccat catcgaaccg ggtctttact tcgcgatgaa
    12121 taccccgcct gctggccttg gcatcaccat gcctaacctg catgaaatgg gtggcgagaa
    12181 cgcgccgatc atcatggcgc agctgaaaga cgttaccgca cacgcggcag cgaccgtcag
    12241 ctcctggggc ttcgtgattt cgccagagca gatcctgcaa accgcgaaag acattggtga
    12301 gccttctgtc ctgaaccgtg caggtggtgc gccaacgctg gcggtaggta tcgctcacgt
    12361 gttccacaaa gtgctgccga tggctgacat gggcttctgg tatcacttcg gtattctgtt
    12421 cgaagccctg ttcatcctga ccgcgctgga tgcgggtact cgttctggcc gctttatgct
    12481 gcaagacctg ctgggtaact tcatcccgtt cctgaaaaaa accgactctc tggttgccgg
    12541 tatcatcggt actgcgggct gtgtgggtct gtggggctac ctgctgtatc agggcgtggt
    12601 cgatccgctg ggcggcgtta agagcctgtg gccgctgttc ggtatttcca accagatgct
    12661 ggcagccgta gcgctggtac tgggcaccgt tgtgctgatt aagatgaagc gcacccaata
    12721 catctgggta actgttgttc cggctgtatg gctgcttatc tgcaccacct gggcgctggg
    12781 tctgaaactg ttcagcacca acccgcagat ggaaggcttc ttctacatgg caagccagta
    12841 caaagagaag attgctaacg gtactgacct gacggcgcag cagattgcca acatgaacca
    12901 catcgttgtg aacaactaca ccaacgcagg cctgagtatt ctgttcctga ttgtggtgta
    12961 cagcatcatc ttctacggtt tcaaaacctg gctgaatgtg cgtaacagcg acaaacgtac
    13021 tgacaaagaa acaccgtacg ttccaatccc ggaaggcggc gtgaagatct cttcgcacca
    13081 ctaaccgtat ttagccccgc ttcggcgggg ctttgttctt atcagagtga actatgtttg
    13141 gtaacttagg acaggcaaaa aaatatctcg gccaggcggc gaagatgttg attggcattc
    13201 cagactacga caactacgtc gagcatatga agaccaacca tcccgacaag ccgtacatga
    13261 gctatgaaga attcttccgc gaacgccaga acgcacgcta cggcggcgat ggtaaaggcg
    13321 gtatgcgctg ttgttaatgg agagagcatg aacccgattg cagttaccct actcaccggt
    13381 tttttaggcg cgggcaaaac caccctgctg cgccatattc ttaatgaaca acacggctac
    13441 aagattgccg tgattgaaaa cgaattcggc gaagtctccg ttgatgatca attgattggt
    13501 gatcgcgcaa cgcagatcaa aacgctgacc aacggctgca tctgctgttc gcgctccaac
    13561 gagctggagg acgcgctact cgacctgctg gataatctcg acaagggcaa tattcagttc
    13621 gatcgtctgg tcattgaatg caccggcatg gccgatcctg gcccgattat tcaaaccttt
    13681 ttctcccatg agattttatg ccagcgctac ctgctggacg gcgtgattgc gctggtggat
    13741 gcggtacatg ccgatgagca gatgaaccag ttcaccatcg cccagtccca ggtgggctac
    13801 gccgaccgta ttctgctgac taaaaccgac gtcgcaggcg aagcagaaaa actgcgtgaa
    13861 cgcctggcgc gcatcaacgc ccgtgcaccg gtctacaccg tcacccacgg cgacatcgac
    13921 ctgggcctgc tgttcaacac caacggtttt atgctggaag aaaacgtcgt cagcaccaaa
    13981 ccgcgtttcc actttatcgc ggataaacaa aacgatattt cgtcgattgt ggtggaactg
    14041 gattacccgg tagatatcag cgaagtttcc cgggtgatgg aaaacctgct gctggaatcg
    14101 gcggataaac tgctgcgtta caaagggatg ctgtggattg acggcgaacc taaccgcctg
    14161 ctgttccagg gcgtccagcg cctctacagc gccgactggg acaggccatg gggcgatgaa
    14221 acaccgcata gcacgatggt gtttatcggg attcagttgc cggaggatga gattcgggcg
    14281 gcgtttgcgg gactcaaaaa gtaaccagcc ctccaacatc acaatacctg agactttatc
    14341 accctcatat tcctccagaa tatgagggct ttcgggctta attatctctg aacgtgcaac
    14401 acttcctgcg actccttatg cgcctccaca tcctgcttcg ttaaccagag cgacttacgg
    14461 ccaaaatttt cgtacatttt cagctgatct tcatagtgct tatcaactgt tccatcggga
    14521 gcaataaacc cactctgacc gggtgcgacc acatcccagg caagcacagg acgatcgctt
    14581 gtcgttggtg agaaaacaat catatcgttt tctgttccac ggttttgata ctccgcctga
    14641 tgacgcgttt cttccgctgc ggcctgcggt acaccaaaga aattatttgc ccggaacgtt
    14701 aaggccattg caggtgtttt ccagttactc acattattgc catagcgttt ggaaagagtc
    14761 tcccaggtat cttccagcgc agccaacaca acctcctgct gtggtttccc agcaaacaga
    14821 tcaaccgcct gtgggattgg tgatttgtct ccctgcaccg cctcatacaa aatttttgct
    14881 ccaacactta tattcagcga accagttggg ccgtcctggg ttgtttcgta gccactggcg
    14941 ctgtaccact tatcaaatgg cataggtacg gcagccacta cggtacgctt caacatactg
    15001 gtcagccaaa cgttcaggat ggcagagcct ggctgctgcc aggttttacc atcatcatta
    15061 agcaaattga tgccatccca acgtgttaat gtttctacca actgacgacg cggatcgctc
    15121 tgtgtcaaac cagatgtcgc tgcttgcaga gtaggtaaaa aaagcctcag gttaagatcc
    15181 tgacgactgg tttggcgaat aacatcccat gcctgatcag cagttaagcg tggcttttgc
    15241 tcaagcagtc ggtcgatctc cgtaacgcga tctgcaccac cccacaaaaa ggcaaacaga
    15301 tctgaagcgg gataatcttt ttggggagaa ttgttccagt tagcaatata tcccgactgg
    15361 gggttataca ccttagggtt catttcaaaa ggcaatagcc ctttccagtc ccattttccc
    15421 gtaccaggaa cgggtaatcg cggatcatgg cctgattgac gatctggata agcaccagta
    15481 tgaacataac caatattgcc gtttacatca gcatagtacc agttgatggt cagtgcttgt
    15541 ttcgctgcct gctgtgtcca ctcctgccaa tttttggcct tcatctgatg agtccaggcc
    15601 agcaaagacg ccacctcttt accatcccat gcgcgggatt tagcgtaagc cgtttgtgtc
    15661 gtctggtcag tttggagaat gttgccatgc accgtacgcc agacagtaaa ggtctctgcc
    15721 tgaccatttt tcaccgtaat ggtttcctca cggcttaaca ttttcaccca cttaccatta
    15781 tgcaagtagt agcctggttt ctctgccgac agccgttcag caaaaatatc gacatcatcg
    15841 ccgaaacctg ccgttgatcc ccaggaaatc acaccattat gaccaaaaac cagcccagga
    15901 taggcaaatg gtgtattgcc agtgacatca taaccagcac cgtgcagacc aataccataa
    15961 gtatacgcag gcgcatacca gccaaactgc ggaccattta ccatgattgc tttcgcatcc
    16021 tgggctttgc ttttgccgat cacccacata ttgctggtcg ttggataccc cgccagacca
    16081 ttggcaccac cctgtgcaaa ttgtgcagca atagtttccc ggttcttccc tgctgttaac
    16141 gccagcagtg cgccatccgc cccttttgct ggtcggtcaa gcattggtgc aggtaaatcg
    16201 tagcgtggca acagagctgc tgtttgcgag ttttgctgat taaatttaag tgggtagtta
    16261 ctctcttgta cggcaatagt ggttggcgct gatgggttta ccagccattt caactgatta
    16321 aataccgcca tgccttgtga tacaccatat ttatctttta aagccgttag cagtgccaga
    16381 ttatcaattt cgctagtgct atcagagaag cggtttgcca tggtgcccac aaatatcatc
    16441 gcgacatcaa acggttccca gcgcttagga gtaaagccaa atgtattaaa ctgttttggt
    16501 aagagcgtct ctggattggt atttacctta tcaatccagg cattcattcc atcagcgtag
    16561 ccttgcagaa tggacatatc ctctggggaa agggcagcaa tttgcgcccg gatagcatcc
    16621 ggccagtagt tacgacggat atctttatca aatttcacaa aatctttgcc aagcacttcc
    16681 gcgacagtcc cttgagtact gcgacgtgcc atttccatct gaaaaaggcg atcttgtgct
    16741 actacatagc catagccata aaataggtgc catgtatcat tggcataaat atgcggcatg
    16801 ccgtattcat cgcgaacaat ctttatctca cttgacgact gctcagccag tgcaggtaag
    16861 ctccaataat acatcaggga agcagtaaca cagttcacga tcatacgatt tctatttttc
    16921 attgtatcct ctggcaggtg tataattagc gaattgatac tagcaacgaa gcttcggtta
    16981 tctatttgca gagaattgtt tcattatgaa ctgtgagcgc gataactaat ttttgataca
    17041 aaaatttcat taatgtgatc taaaacgaat cgctttattc accgctgatg attttgttct
    17101 attccttttt gtgacagatc ccccggacgc atagatgatg acccccagag agacagctca
    17161 ggtcattatc tgtatgtaaa tcagaaaaat atgttgtaaa cgacttttat ggttggattt
    17221 tgtagcaatt ttgaatgcag acaggcagca tttgccaaca cgccgcccat agttcactcc
    17281 acatcctccc tgcgtaaccc aatatccctc aaccgttcat cactcatctg ctgtaacact
    17341 cgtcgggtct gcatttgccg ccgccaacgc ctcaccgcat gccagagttg cacaagtctg
    17401 ataaacggcg ttttggcttt gttttcgtga aattccatct gctgcctcct catctttcag
    17461 aggcttcatc atccgccttc tggcagttca caatacagat gcaaatatta gttttattta
    17521 acatacagac aggttaaaac agcatctgca tccggttttt tagcctcatc tgtaccggtt
    17581 tttctttctg tatgggccaa ataaggaata cagcatgacg cgttatcaac atctggcgac
    17641 tctacttgcc gagcggattg agcaagggct gtatcgtcac ggggagaaat tgccgtcggt
    17701 gcgcagctta agtcaggagc acggcgtcag catcagcacc gtgcaacagg cgtatcagac
    17761 gctggaaacg atgaggctca tcactccgca gccgcgttcg ggttattttg tcgcacaacg
    17821 taaagcccag ccgcctgtac cgccgatgac gcgtccggtg cagcgcccgg tggaaattac
    17881 ccagtgggat caggtgctgg atatgctgga ggcgcatagc gacaattcca ttgttccgtt
    17941 aagcaaaagc acgccggatg tcgaaacgcc cagcctgaaa ccgctgtggc gtgagctaag
    18001 ccgggtggtg cagcataatc tgcaaaccgt tctcggttat gacttgttag ccggtcagcg
    18061 agtgttgcgg gagcagattg cccgcctgat gctcgacagc ggctcggtgg tcaccgccga
    18121 tgacatcatc atcaccagcg gctgccataa ctcgatgtcg ctggcgttaa tggcggtgtg
    18181 taaaccgggc gatattgtcg cggtcgaatc tccctgttat tacggttcga tgcagatgct
    18241 gcgaggcatg ggcgtgaaag tgattgaaat cccaaccgat ccagaaactg gcatcagcgt
    18301 tgaagcactg gaactggcgc tggaacagtg gccgattaaa ggcatcattc tggtgccaaa
    18361 ctgtaataat ccgctgggat ttattatgcc ggacgcacgc aaacgggccg ttctctctct
    18421 cgctcagcgt catgatattg tgatttttga agatgatgtc tacggcgaac tggcaacgga
    18481 gtatccgcgc ccgcggacca tccattcatg ggatatcgac gggcgagtgc tgttgtgcag
    18541 ctcgttcagt aaaagtattg ctccaggcct gcgcgtgggt tgggtcgcac cggggcgtta
    18601 tcacgataaa ctgatgcata tgaaatacgc catcagcagc tttaatgtgc cgtccacgca
    18661 aatggcggcg gcaacgtttg tgttggaagg ccactatcat cgccatatcc ggcggatgcg
    18721 gcagacttat cagcgcaatc tggcgcttta tacctgctgg atacgggaat attttccctg
    18781 cgaaatctgt attacgcgcc cgaaaggcgg atttttactg tggatagaat tgcctgaaca
    18841 ggtcgatatg gtctgcgttg cgcggcagct gtgccgcatg aatatccagg tggcagcagg
    18901 ctcgattttc tcggcttccg gcaaataccg taattgtcta cgcatcaact gcgctttgcc
    18961 gctcagcgaa acctatcgcg aagcactaaa gcaaattggc gaggccgtgt atcgggcaat
    19021 ggaataaatt cgaattagtg gcgaagcacc tgatttcctc tcacgaatcg cctgtaactc
    19081 gttgtatctt tgcagggtca tcgccacgtt ccagcatggc ttaggcaaag tgtgcttcgt
    19141 tcataccgga tgcggcgtga acgccttatc cggcctacaa accgcgcaaa ttcaatagat
    19201 tgcagaggaa atgtaggtct gataagcgta gcgcatcagg cagctttgcg tttgtcatcc
    19261 gtctctgatt agtattcgat ccatggatcg cgcttgattt caggcaaggg ctgctctaat
    19321 gcctgcattt gctctgtcga aagtccggta atcttttgta tccattcagg atctgcccca
    19381 ttctgcaaca acaatctaag gaggcgttgt tccccttctt ctttcccctt ttccatcccc
    19441 aacagccatc catcattata aattcgctct gcaatcgtca ttattcgctc cctgtgttgt
    19501 ggcattcgac gggtaagttc gctgataaac gccttaaaac gcgcttcatc gccagtcagt
    19561 aaaatgtaat ttaacagcgc agttatctgg ctgtcattag cacactctgt aaccagtaat
    19621 actaccagtt ggtcaataag ccccatcaga tcgcgctggc gaatatgctt ttggatcaac
    19681 tccaacaggg cgactctgcg atgctgcaca atctcgtcgt ctggcacgac agtgacatcc
    19741 accagcggga acgctgcgct ataaagtttc cgtgcggtag tcggggcggc aaattcgtcc
    19801 agccagcaca gggaccaggg gtaaggacta cggctaccgt gataaaacag catcgggata
    19861 accagcggta gcggccggcg tttatcatgc tcgatatggc gctgcatcac cgccatggaa
    19921 tatcgcatca ggcgaaacgc catatgaata tcctcgcggc tctgatgttc aatcactacg
    19981 taaatataac catcaccttc acgggtcttt accgaccaca gaatatcgga gtgtagcgcc
    20041 cgcaattttt catcgacaaa gctggcggat tccagtttta agctgtcgag atcgcacagt
    20101 tcacgtaaat ctttgggtaa gtggatctcc ataaaatccc gcgcggtgtc agggtgagtg
    20161 agaaaggatt taaataatgc gtcatgtggc gtgctggtcg tgaagtttgt catggcgatc
    20221 cgtcacctgt agataagatg gcccgacttt agcgactaac aaaaccattc tcacccggca
    20281 atttctcgtt ccccgaggtg ctttccaggc tatttttgtt cgtctgcaaa cgatacattt
    20341 ttccggaaac aggctcgcgt tttacatttc ctgtacccgg cagatattcc cctttccacc
    20401 gcgctctttt gattaaccgc aaattttaat tacacttaag gtgtatattt tctatgcaac
    20461 ccatcaatta aagaggtgta atgtgctgat gactatttgt aatcgttata cattctgacc
    20521 tggagtcaga aggtatttct ctgtctgtgt gttcacaggc agtgtggttg attacatgaa
    20581 attcagtaca tttgtccttc tcgcctgcct ttcgtcatta ccgacggtat tgaatttcgt
    20641 tttccccgtt ggggttctcc ggacaaggag ttgtttgtta tgccacgttt ttttgcccgc
    20701 catgccgcca cgctgttttt cccgatggcg ttgattttgt atgactttgc cgcgtacctg
    20761 tcgacggatc tgatccagcc ggggatcatt aatgtggtcc gtgattttaa tgccgatgtc
    20821 agtctggccc cggcagccgt cagtctctat ctcgcgggcg gtatggcgtt acagtggctg
    20881 ctggggccgc tttccgacag gattggccgc aggccggtgc tgattaccgg ggcgctaata
    20941 tttacccttg cctgcgctgc gacaatgttc acaacgtcta tgacacagtt tcttatcgcg
    21001 cgtgcaattc agggcaccag tatctgtttt attgccaccg ttggttatgt cacggtgcag
    21061 gaggcgttcg gccagacaaa agggatcaag ttgatggcga ttatcacctc catcgtactg
    21121 attgcgccga ttattggccc gctttccggc gcagctctga tgcactttgt gcactggaaa
    21181 gtcctttttg ccatcattgc agttatgggt tttatctcat ttgttggctt actgctggcg
    21241 atgccagaga cggtgaagcg cggcgcggtt ccatttagcg ccaaaagcgt ctttcgcgat
    21301 tttcgtaatg tcttttgcaa tcggctgttc ctctttggcg cagcaaccat ctctttaagc
    21361 tatatcccaa tgatgagttg ggtggctgtc tcgccggtga tcctgatcga cgcgggcggc
    21421 ttaacaactt cgcagttcgc ctggacacag gttccggtgt tcggcgcggt gattgtcgcg
    21481 aatgccatcg tggcgcgttt cgttaaagat ccgaccgaac cgcgctttat ctggcgtgct
    21541 gtacccattc aactggtcgg cctcgcgctg ttgattatcg gcaatctgct gtcaccgcac
    21601 gtctggctgt ggtcggtgct gggcaccagt ttgtatgctt tcgggattgg tttgatcttc
    21661 ccgaccttat tccgctttac gcttttttcc aataacttac cgaaagggac cgtctccgca
    21721 tcgctgaata tggtgatcct gatggtgatg tctgtctcgg tcgaaatcgg ccgctggctg
    21781 tggtttaacg gcggtcgctt gccgtttcat ctgttagccg tcgtggcggg cgttatcgtc
    21841 gttttcaccc tggcaggatt gctcaatcgc gtgcgccaac atcaggctgc cgagctggcg
    21901 gaggagcggt gatttttgcg cgatcctgcc gtcaggctct attcttaacg ttatgaataa
    21961 actcattgaa ctcagacgcg ccaaaatgtt ggcgctctct ttactgctta tcgccgctgc
    22021 tacctttgtc gttacgctgt ttttgccgcc caatttttgg gtgagcggcg tgaaggcgat
    22081 tgctgaagcg gcgatggtcg gcgcgctggc ggactggttt gcggtggtgg cgctgtttcg
    22141 ccgcgtgccg attccgatca tttctcgtca tacggcgatt atcccgcgta ataaagaccg
    22201 gattggcgaa aatctcggcc agttcgtgca ggaaaaattt ctcgataccc agtcgctggt
    22261 ggcattgatt cgacgccacg aaccggtgtt gttgattggc aactggttta gtcagccaga
    22321 aaacgcccgc cgcgttggtc agcatctgtt gcagatcatg agcggttttc ttgaactgac
    22381 cgatgatgcg cgtattcagc gcctgcttaa gcgcgcggtc catcgggcga ttgataaggt
    22441 cgatctttcc ggcaccagtg cgttgatgct ggagagtatg accaaaaacg atcgtcatca
    22501 ggtgctactg gatacgctga tcgcacagtt gatcgccctt ctccagcgcg ataaatcgcg
    22561 caagtttatt gcccagcaaa ttgttcgctg gctggagagt gagcatccac tgaaagccaa
    22621 aattctcccc accgaatggt tgggcgaaca tagcgcggag ttggtttctg acgcggtgaa
    22681 ttctttgctt gatgatatca gccgtgatcg tgcgcatcag atccgtcatg cgtttgatcg
    22741 cgccactttt gccctgatcg acaagttgaa aaacgatccg gaaatggcag cgcgagccga
    22801 tgccgtaaaa agttatctga aagaagatga agcttttaac cgctatctca gtgaattgtg
    22861 gggggattta cgggagtggc tgaaagcgga tatcaacagt gaagattctc gtgtgaaaga
    22921 acgtatcgcg cgggcgggtc aatggtttgg cgaaacgtta attgccgatg atgccttgcg
    22981 ggcgtcgtta aatggtcacc tggaacaagc cgcgcaccgc gtcgcgcctg agttttccgc
    23041 attcctgacg cgccacatca gcgatacagt aaaaagctgg gatgcgcggg atatgtcgcg
    23101 gcaaatcgag ttaaatatcg gcaaagatct gcagtttatc cgtgtcaacg gtacgctggt
    23161 tggcggttgt attgggctaa ttttatattt gttgtcgcag ctcccggcct tgttccccct
    23221 cagcaatttt tagaaatcca tgaaaaacta agtggctaac cgcgagagag atcaaataaa
    23281 ccacactaaa agaatggtaa taatgtggcc tcttttcatt atctcccgtg gtacggagaa
    23341 ggaaaatcat gtcacttatc accgatctac ccgccatttt cgatcagttc tctgaagctc
    23401 gccagaaagg ctttctcacc gtcatggatc tcaaggagcg cggcattccg ctggttggca
    23461 cttactgcac ctttatgccg caagagatcc cgatggcagc cggtgcggtt gtggtttcgc
    23521 tctgttctac ctctgatgaa accattgaag aagcggagaa agatctgccg cgcaacctct
    23581 gcccgctgat taaaagcagc tacggctttg gcaaaaccga taaatgcccc tacttctact
    23641 tttcggatct ggtggtcggt gaaaccacct gcgacggcaa aaagaaaatg tatgaataca
    23701 tggcggagtt taagcccgtt catgtgatgc agttgccgaa cagcgttaag gacgatgcct
    23761 cgcgtgcgtt atggaaagcc gagatgctgc gcttgcaaaa aacggtagaa gaacgttttg
    23821 ggcacgagat tagcgaagat gctctgcgcg atgccattgc gctgaaaaac cgcgaacgtc
    23881 gcgcactggc taatttttat catcttgggc agttaaatcc tccggcgctt agcggcagcg
    23941 acattctgaa agtggtctac ggcgcaacct tccggttcga taaagaggcg ttgatcaatg
    24001 aactggacgc gatgacagcc cgcgttcgtc agcagtggga agaaggccag cggctggccc
    24061 tgcgtccacg cattttaatc accggctgcc cgattggcgg cgcagcagaa aaagtggtgc
    24121 gcgcgattga agagaatggc ggctgggttg tcggttatga aaactgcacc ggggcgaaag
    24181 cgaccgagca atgcgtggca gaaacgggcg atgtctacga cgcactggcg gataaatatc
    24241 tggcgattgg ctgctcctgt gtttcgccga acgatcagcg cctgcaaatg ctcagccaga
    24301 tggtggaaga atatcaggtc gatggcgtag ttgatgtgat tttgcaggcg tgccatacct
    24361 acgcggtgga atcgctggcg attaaacgtc atgtgcgtca gcagcacaac attccttata
    24421 tcgctattga aacagactac tccacctcag atgtcgggca gctcagtacc cgtgtcgcgg
    24481 cctttattga gatgctgtaa ggagtggcag tgacatattc gattggcatt gattctggtt
    24541 ctaccgccac caaagggatc ttactggcag acggcgtgat tacgcgccgt ttcctcgttc
    24601 caacaccctt tcgtccggca acagcaatta ctgaagcctg ggaaactctg cgcgaagggt
    24661 tagagacaac gccgtttctg acgctcaccg gctatggacg gcaactggtg gattttgccg
    24721 ataaacaagt gacggaaatc tcctgtcatg ggctgggcgc acggtttctt gcgccagcaa
    24781 cgcgcgcggt aatcgacatc ggtggtcagg acagcaaagt gattcagctt gatgatgacg
    24841 gtaacctgtg cgatttcctg atgaatgaca aatgcgcggc gggcaccggg cgtttcctgg
    24901 aggtgatctc gcgcacgctt ggcaccagcg tcgagcaact cgacagcatt accgaaaatg
    24961 tcacgccgca cgccatcacg agtatgtgca cagtgtttgc cgaatcagaa gtgatcagcc
    25021 tgcgctcagc gggcgtcgcg ccagaagcga ttctcgccgg agtgattaac gcgatggcgc
    25081 ggaggagtgc caatttcatt gctcgtctct cctgtgaagc gccgattctg tttactggtg
    25141 gcgttagtca ttgccagacg tttgcccgga tgctggaatc tcatctggga atgccggtaa
    25201 atacccatcc tgatgcgcaa tttgctggcg caattggcgc ggcggtaatt ggtcaacgag
    25261 tgaggacacg ccgatgaaag agtttttatt tttatttcac tccacggtcg gcgtcataca
    25321 aacccgcaag gcgttgcagg cagcgggcat gacttttcgc gtcagcgata ttccccgtga
    25381 tttacgcggc ggctgcgggt tatgcatttg gctgacctgt ccccccggcg aggaaataca
    25441 atgggtgatc cctgggcaga ctgagtcaat ttattgccag caggatggtg tctggcgctg
    25501 cattgcacat tacggggttt ctccgcgtta aggattcatt aaggtcgcta cgtatacttc
    25561 tcgatatctt attgatttgc cttaaaggga ttaccgtgac aaaaagtatt tcgttaagta
    25621 aacgcatctc cgttattgtg attctttttg ccattgtggc agtgtgtact ttcttcgtgc
    25681 aatcttgcgc ccgtaaaagc aatcatgcag catcatttca gaattaccac gccaccattg
    25741 acggcaaaga aattgcaggc ataacgaata atatctcttc gctgacctgg tcggcacaaa
    25801 gcaatactct ttttagcact atcaacaaac ctgccgccat tgttgaaatg accaccaatg
    25861 gtgattttat ccgcacaatt cctctggatt ttgttaagga tctcgagacg atcgaataca
    25921 ttggcgataa tcagtttgtc attagcgatg agcgcgatta cgccatttat gtgatttcac
    25981 tgaccccaaa ctcggaagta aaaatcctca aaaaaataaa aatcccgctg caagactccc
    26041 caaccaattg cggttttgaa gggttggctt attctcgcca ggatcacact ttctggtttt
    26101 ttaaagagaa aaatccgatt gaggtctaca aagttaacgg gctgttaagt agcaatgaat
    26161 tacacatcag caaagacgag gcactacaac ggcaatttac tttagatgat gtgtccggcg
    26221 cagaatttaa ccagcaaaaa aatacgcttc tggtgctatc tcatgaatct cgcgcactcc
    26281 aggaagtaac cctggttggc gaggttatcg gtgaaatatc actaaccaaa ggtagtcgag
    26341 gtttgtcgca taacatcaaa caggccgaag ggattgcgat ggatgcctct ggtaatattt
    26401 atatcgttag cgaaccaaat cgtttttatc gctttacccc taaatcatcg cattaacagg
    26461 ctggcatatt tcaccttttt gttattaact tgttaacaac aaaaatgaca tacaaggtta
    26521 aacatgcctt cgtcctcgca tcccgtagaa cgcttttctt tcagcaccgc gcttttcggg
    26581 atgctggttc tgaccttagg tatgggatta ggtcgcttcc tttatacgcc tatgttgccc
    26641 gtcatgatgg cggaaggggc gttttcattt agccagctct cgtggattgc cagcggcaac
    26701 tatgcggggt atctggctgg cagtctgcta ttttcgtttg gcgcatttca ccagccgtcg
    26761 cgcctgcgcc cattactgtt ggtttctgcc ctggcgagcg ggttgttgat cctcgcaatg
    26821 gcatggttgc cgccatttat tctggtgtta ttgattcgct tcctggcggg tgtcgccagc
    26881 gccggtatgc tgatttttgg ttcgacgctg attatgcagc acacgcgcca tccttttgtg
    26941 ctggcagctt tgttttctgg cgttggcatt ggcattgcac tgggcaacga atatgttctg
    27001 gcaggcctgc attttgcctt ctcctcgcaa acgttatggc aaggcgccgg ggcgctttct
    27061 ggcatgatgc tgattgcact tacgctttta atgccctcga aaaaacacgc catcgcacca
    27121 atgccattgg caaaaacgga gcaacagata atgagctggt ggttactggc tattctgtac
    27181 ggccttgcgg gatttggtta tatcatcgtc gcgacttacc tgccgctcat ggcgaaagat
    27241 gcaggctcac cattgttaac cgcccatctc tggacgttag tcggcttatc aattgtgcca
    27301 ggctgctttg gctggctatg ggcagcaaaa cgttgggggg ctttgccttg cctgacggcg
    27361 aatttgctgg tgcaggctat cagtgtgctg cttactctcg ccagcgactc tcctctcttg
    27421 cttatcatta gtagtatcgg ttttggcggt acctttatgg gcacgacatc tctggtgatg
    27481 actatcgccc gccaactgag cgttccggga aatctcaatc ttttaggctt tgtgacgctc
    27541 atttacggca ttggacaaat cctcggcccg gcgctgacca gtatgctcgg caatggaaca
    27601 tcggcgcttg ccggcgccac tctctgcggc gcggcggcgt tgtttattgc tgcgcttatc
    27661 agcacagtac aattatttaa gctgcaagtg gtcacttcat gaggagtgtt tcccttacca
    27721 ctcaagaaac tcaacagcaa ccgttgatgt cagccagacc ccgttttccg cttgccagaa
    27781 agggatgcct cgtttcgcca tttcctgtgc tttgacggtt aagataaccg gcgatccgtg
    27841 tcttgctccc actttgcgtg ccgttgcttc atcagcagag agatgtacgt aatgacgctc
    27901 ccctgcaatc agcccctgtt tttttatctc gtcaaggaaa cggcttgccg taccgtggta
    27961 gagaaactgt ggcggtgttt tttcggcaaa ggaaatggcg acctgcgaag tcgaatgccc
    28021 ctgaaccgcg cggatgcacc taccatcact ggaataacta aaacgctttt tatcgctggt
    28081 ggcaaccaca gtatccagca atgcacgggt taaccgcttg cccgcttttt gggcacaaag
    28141 tatgagctta tcgatatccg cccatccttc acggtcaagt acgataccaa tcgcctccgg
    28201 tttgtgtcgg aggacaaaac ttaaaaactt gctcgtatcc gccaactctt tttcgttgta
    28261 ctttgccatc gcgttttcgt ccctggattt tctgttttta tttactgatg tcattgtcac
    28321 tgggtttatg aattttctgc aactcaatcc tgcgcagagc cttttctaca cagcgtaatt
    28381 tttccgctat gcaaaaaacg catagcaaga aacatacttg cattttcccc atacccacat
    28441 ggctgataag gtttttcagc tccaggaatt ttcctggctc aagcaacaca caaacacaaa
    28501 aaaacaatgg ggattgttat gacacaacaa ggggatgctg tagccgggga gctggcgaca
    28561 gaaaaagtgg gcattaaagg gtatctggct ttttttctca ccataatctt tttttccgga
    28621 gttttttccg ggacagacag ctggtggcgc gtttttgatt tttccgttct gaacggatcg
    28681 tttgggcaac tgcctggtgc gaatggagcc accacctcat ttcgcggggc gggtggcgcg
    28741 ggggctaaag atggctttct ctttgcgctg gagctggcac cgtcagtcat tctctctctg
    28801 gggattattt caattaccga tggtcttggt ggattacgcg ccgcgcagca gttaatgacg
    28861 cctgtgctaa aaccgttgtt gggcattcca gggatctgct cgctggcgct gatcgctaac
    28921 ctgcaaaaca ctgatgccgc cgccggtatg accaaagagc tggcgcagga aggggaaatt
    28981 accgaacgcg ataaagtgat tttcgccgca tatcaaacca gcggtagcgc catcatcacc
    29041 aactacttct cttccggcgt cgcggtgttc gcctttctgg gcacgtccgt aatcgtacct
    29101 ttagccgtca tcctggtgtt taaatttgtc ggagccaaca ttttgcgcgt ctggcttaac
    29161 ttcgaagaac gtcgcaaccc aacgcaagga gcgcaagcat gacaactcag gtccgcaaaa
    29221 acgtcatgga tatgtttatc gatggcgcgc gccgtggctt tactatcgcc acgaccaacc
    29281 tgctgccaaa cgtggtgatg gcgtttgtca tcattcaggc gctgaaaatt accggcctgc
    29341 tcgactgggt ggggcatatc tgtgaacccg taatggcgct gtggggatta cccggtgaag
    29401 cggcaaccgt ccttttagcc gcattaatga gtatgggcgg cgcagtcggt gttgccgcca
    29461 gtctggcgac cgccggcgca ttaaccggtc acgatgtcac cgttttactg cccgcgatgt
    29521 atttaatggg caacccggtg caaaatgtcg gtcgctgcct ggggacagcc gaagtgaatg
    29581 ccaaatatta cccgcatatc attacggtct gcgtgattaa cgcattgctg tcgatctggg
    29641 ttatgcagct tattgtttaa caaggagtta ccatgattga ttataccgca gccggtttta
    29701 ccctgctgca gggagcgcat ttgtatgcgc cggaagatcg gggaatttgc gatgtcctcg
    29761 tcgctaacgg caaaattatc gccgttgcca gcaatatccc ttctgacatt gtaccggact
    29821 gcacggttgt cgatctcagc gggcagatcc tctgtcctgg ttttattgat caacacgtcc
    29881 atttgattgg tggtggcggc gaagcaggtc ccacgacgcg cacgccagaa gtggcgctaa
    29941 gtcgcctgac ggaagcgggc gttacgtcag tggttggtct gctgggcacc gactctatct
    30001 ctcgccaccc ggaatccctg ctcgccaaga cccgtgcgct caatgaagaa ggcatcagcg
    30061 cctggatgct gaccggcgct tatcatgtcc cttcccgcac cattacaggt tccgtggaaa
    30121 aagacgtggc gattatcgat cgtgtgattg gcgtgaaatg cgccatctct gatcaccgtt
    30181 ctgccgcacc ggacgtttat cacctggcta atatggcggc ggaatcccgc gttggcggtt
    30241 tgctcggcgg taaacctggc gtcaccgtgt tccacatggg cgacagcaaa aaggcgttac
    30301 agcctgtcta tgacctgctg gaaaactgcg atgtgccgat cagcaagttg ctgccgaccc
    30361 acgttaaccg caacgttccg ttgtttgagc aggcgctgga gttcgcgcgc aaaggcggca
    30421 ctatcgatat caccagcagc attgacgaac cggtcgcccc tgccgaaggt attgcccgcg
    30481 ccgttcaggc gggtattccg ctggcacgcg tcaccctcag ctccgacggc aacggtagcc
    30541 agccgttctt cgatgacgaa gggaatttaa cccatatcgg tgttgccggt tttgaaacgt
    30601 tgctggaaac cgtgcaggtg ctggtcaaag actatgattt cagtatcagc gatgccctgc
    30661 gcccgctcac cagtagcgta gccggtttcc ttaacctgac cgggaaaggc gaaattctgc
    30721 caggcaatga tgctgacttg ctggtcatga cgccagaact gcgcattgag caggtatacg
    30781 ctcgcggcaa actgatggtc aaagacggca aagcctgcgt gaatggaacg tttgaaacgg
    30841 cttaaacggt tgtcagccgc ccgaatggac gtagaatgcc cgcaagggcg gctgacagag
    30901 taaaacgtaa tggatgactg tggtgcgatt ttgcataata ttgaaaccaa atggctttat
    30961 gattttctga ccctggaaaa atgccgcaat ttttcccagg cggcagtcag tcgcaacgtc
    31021 tcgcaaccgg cattcagccg ccgcatccgt gcgctggaac aggcgattgg tgttgaattg
    31081 tttaaccgcc aggtaacgcc gctgcaactc tcagaacaag ggaaaatctt ccattcgcag
    31141 atccgccatc tgttgcaaca gttagagagc aatctggcag agctgcgtgg cggcagcgat
    31201 tacgcgcaac gaaaaatcaa gatagccgct gcacactctc tttccctcgg gctgttaccg
    31261 tccattatca gccagatgcc gccgctcttt acctgggcaa ttgaagctat tgatgtcgat
    31321 gaagcggtcg ataaactgcg tgaagggcaa agtgactgta ttttttcctt tcacgacgaa
    31381 gatttgctgg aagcgccgtt tgaccacatt cgcttatttg aatctcaatt gttccctgtc
    31441 tgcgccagtg acgaacacgg agaagcactt tttaacctcg cgcagccaca ctttccgtta
    31501 ctgaattaca gccgcaactc ctacatgggg cgattgatta atcgcaccct gacgcgccac
    31561 agtgagttaa gtttcagcac cttttttgtc tcttcgatga gcgagctttt aaagcaggtt
    31621 gccctcgacg gctgtgggat tgcctggctg ccggagtacg ccatccaaca agaaattcgc
    31681 agcgggaagc tcgttgtact gaatcgggac gaactggtga tcccgattca ggcctatgca
    31741 taccgaatga atacccgcat gaatccagtt gccgaacgct tctggcgtga actgcgcgag
    31801 ctggagattg tgcttagctg acattctcca gcatcgcact acaccaaccg ccattatccc
    31861 agaccagatc aaacgttagc ggttcaccgt cgtggtacag cattatctca aggctgatcg
    31921 ccagcgtgtg gctgtacgct tctttcagat tgacctgcaa cgccgcaatc cgtggctcat
    31981 gaaataacag tgcagaacgc agctgacggc aataccagct ctggagatgt ttgtcgctct
    32041 ggttatgcac ttgccactcg ggcatgcctg aaacaagtaa tgacggtaat gccgaatgag
    32101 gagaattaaa aagagtgagg agatcgctac atactgattt acgcagcgat gacgttttat
    32161 tgcctgaaat ttcaggtaaa acagcctgaa gtgattggcg catcattttg cgcactcctg
    32221 acgtttagca acatatgcgg tttgccctga acaccgggca ccactgtatt gatgctcatt
    32281 ttccatagcc attccctttt gttcattaca tccatgtcag tctgagacag gcctgaatcc
    32341 tgtcagcaaa gtataaaacc ttacttgcct atgaatatct actgactttt caggaaaaaa
    32401 gtcatgattt gataaaaatc atcaaactca aaaaaaaacc gct
//
LOCUS       DAZFQD010000044        32316 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.044, whole genome shotgun sequence.
ACCESSION   DAZFQD010000044 DAZFQD010000000
VERSION     DAZFQD010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32316
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.044"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(18..93)
                     /locus_tag="ACHKTW_002128"
     tRNA            complement(18..93)
                     /locus_tag="ACHKTW_002128"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(58..60),aa:His,seq:gtg)
     gene            complement(152..228)
                     /locus_tag="ACHKTW_002129"
     tRNA            complement(152..228)
                     /locus_tag="ACHKTW_002129"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(192..194),aa:Arg,seq:ccg)
     gene            complement(331..1716)
                     /gene="yifK"
                     /locus_tag="ACHKTW_002130"
     CDS             complement(331..1716)
                     /gene="yifK"
                     /locus_tag="ACHKTW_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFO6463153.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWFPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(1907..2647)
                     /gene="wecG"
                     /locus_tag="ACHKTW_002131"
     CDS             complement(1907..2647)
                     /gene="wecG"
                     /locus_tag="ACHKTW_002131"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047241 - lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HFO6463154.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(2650..4002)
                     /gene="wzyE"
                     /locus_tag="ACHKTW_002132"
     CDS             complement(2650..4002)
                     /gene="wzyE"
                     /locus_tag="ACHKTW_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HFO6463155.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQALLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNREPNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(3999..5078)
                     /gene="rffT"
                     /locus_tag="ACHKTW_002133"
                     /gene_synonym="wecF"
     CDS             complement(3999..5078)
                     /gene="rffT"
                     /locus_tag="ACHKTW_002133"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HFO6463156.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNTMANDRQREGKMTILVGNSGDLSNEHVAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQILSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASVDKN
                     TIAFFSPNYLQGWQRALAIAAGEGA"
     gene            complement(5075..6325)
                     /gene="wzxE"
                     /locus_tag="ACHKTW_002134"
     CDS             complement(5075..6325)
                     /gene="wzxE"
                     /locus_tag="ACHKTW_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HFO6463157.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGYTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEISQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(6327..7457)
                     /gene="rffA"
                     /locus_tag="ACHKTW_002135"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(6327..7457)
                     /gene="rffA"
                     /locus_tag="ACHKTW_002135"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019180 -
                     dTDP-4-amino-4,6-dideoxygalactose transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HFO6463158.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDALAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGERFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(7462..8136)
                     /gene="rffC"
                     /locus_tag="ACHKTW_002136"
                     /gene_synonym="wecD"
     CDS             complement(7462..8136)
                     /gene="rffC"
                     /locus_tag="ACHKTW_002136"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HFO6463159.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPLLTPDVLAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASGRFYAQWIENAVRGTFDHQCLILRAASGGIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(8114..8995)
                     /gene="rfbA"
                     /locus_tag="ACHKTW_002137"
     CDS             complement(8114..8995)
                     /gene="rfbA"
                     /locus_tag="ACHKTW_002137"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFO6463160.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(9014..10081)
                     /gene="rffG"
                     /locus_tag="ACHKTW_002138"
     CDS             complement(9014..10081)
                     /gene="rffG"
                     /locus_tag="ACHKTW_002138"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFO6463161.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSEHFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNTLTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELAPN
                     KPHGVAHYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(10081..11343)
                     /gene="wecC"
                     /locus_tag="ACHKTW_002139"
     CDS             complement(10081..11343)
                     /gene="wecC"
                     /locus_tag="ACHKTW_002139"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0089714 - UDP-N-acetyl-D-mannosamine
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HFO6463162.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSTRASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(11340..12470)
                     /gene="wecB"
                     /locus_tag="ACHKTW_002140"
     CDS             complement(11340..12470)
                     /gene="wecB"
                     /locus_tag="ACHKTW_002140"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-hydrolyzing UDP-N-acetylglucosamine
                     2-epimerase"
                     /protein_id="HFO6463163.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGDLYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIDPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQTMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(12526..13572)
                     /gene="wzzE"
                     /locus_tag="ACHKTW_002141"
     CDS             complement(12526..13572)
                     /gene="wzzE"
                     /locus_tag="ACHKTW_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HFO6463164.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(13584..14687)
                     /gene="wecA"
                     /locus_tag="ACHKTW_002142"
     CDS             complement(13584..14687)
                     /gene="wecA"
                     /locus_tag="ACHKTW_002142"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0036380 -
                     UDP-N-acetylglucosamine-undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HFO6463165.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(14927..16186)
                     /gene="rho"
                     /locus_tag="ACHKTW_002143"
     CDS             complement(14927..16186)
                     /gene="rho"
                     /locus_tag="ACHKTW_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFO6463166.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(16271..16372)
                     /gene="rhoL"
                     /locus_tag="ACHKTW_002144"
     CDS             complement(16271..16372)
                     /gene="rhoL"
                     /locus_tag="ACHKTW_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HFO6463167.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(16513..16842)
                     /gene="trxA"
                     /locus_tag="ACHKTW_002145"
     CDS             complement(16513..16842)
                     /gene="trxA"
                     /locus_tag="ACHKTW_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HFO6463168.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            16973..18238
                     /gene="rhlB"
                     /locus_tag="ACHKTW_002146"
     CDS             16973..18238
                     /gene="rhlB"
                     /locus_tag="ACHKTW_002146"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HFO6463169.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            18372..19856
                     /gene="gppA"
                     /locus_tag="ACHKTW_002147"
     CDS             18372..19856
                     /gene="gppA"
                     /locus_tag="ACHKTW_002147"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008894 -
                     guanosine-5'-triphosphate,3'-diphosphate diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0015974 - guanosine pentaphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HFO6463170.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENALSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVEKEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQESQWQSYVHWPLEVH"
     gene            complement(19903..21924)
                     /gene="rep"
                     /locus_tag="ACHKTW_002148"
     CDS             complement(19903..21924)
                     /gene="rep"
                     /locus_tag="ACHKTW_002148"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HFO6463171.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEARGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPSQAAASAIGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNHQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSPKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            22141..22590
                     /locus_tag="ACHKTW_002149"
     CDS             22141..22590
                     /locus_tag="ACHKTW_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFO6463172.1"
                     /translation="MAINFSPKVGEILECNFGNYPVSQNGQFSTKYYDGRIPPEMIKN
                     RLVVVLNGKINGNACIVVPLSTTRDHDKLNRGMHVEIASNVINGLQFFDQQIRWAKAD
                     LVQQVSRNRLNRARTYRGYLNQCLPHELVADIQRAVIKSINAISLIN"
     gene            22789..23070
                     /gene="ppiC"
                     /locus_tag="ACHKTW_002150"
     CDS             22789..23070
                     /gene="ppiC"
                     /locus_tag="ACHKTW_002150"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HFO6463173.1"
                     /translation="MAKTAAALHILVKEEKLALDLLEQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(23157..24632)
                     /gene="ilvC"
                     /locus_tag="ACHKTW_002151"
     CDS             complement(23157..24632)
                     /gene="ilvC"
                     /locus_tag="ACHKTW_002151"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004455 - ketol-acid reductoisomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HFO6463174.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRCHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            24782..25672
                     /gene="ilvY"
                     /locus_tag="ACHKTW_002152"
     CDS             24782..25672
                     /gene="ilvY"
                     /locus_tag="ACHKTW_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000365800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HFO6463175.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEDDLG
                     QALFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPSLSGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILERSDEKTPFELGVCA
                     QKKRLHEPLIEAFWKILPNH"
     gene            complement(25669..27213)
                     /gene="ilvA"
                     /locus_tag="ACHKTW_002153"
     CDS             complement(25669..27213)
                     /gene="ilvA"
                     /locus_tag="ACHKTW_002153"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HFO6463176.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(27216..29066)
                     /gene="ilvD"
                     /locus_tag="ACHKTW_002154"
     CDS             complement(27216..29066)
                     /gene="ilvD"
                     /locus_tag="ACHKTW_002154"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HFO6463177.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDSLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALRAYASLATSADKGAVRDKSKLGG"
     gene            complement(29131..30060)
                     /gene="ilvE"
                     /locus_tag="ACHKTW_002155"
     CDS             complement(29131..30060)
                     /gene="ilvE"
                     /locus_tag="ACHKTW_002155"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HFO6463178.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQVGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(30080..30343)
                     /gene="ilvM"
                     /locus_tag="ACHKTW_002156"
     CDS             complement(30080..30343)
                     /gene="ilvM"
                     /locus_tag="ACHKTW_002156"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HFO6463179.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(30340..31986)
                     /gene="ilvG"
                     /locus_tag="ACHKTW_002157"
     CDS             complement(30340..31986)
                     /gene="ilvG"
                     /locus_tag="ACHKTW_002157"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HFO6463180.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAAAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLATTKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQQYC
                     AQLRDEHTWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(31989..32039)
                     /gene="ilvX"
                     /locus_tag="ACHKTW_002158"
     CDS             complement(31989..32039)
                     /gene="ilvX"
                     /locus_tag="ACHKTW_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide IlvX"
                     /protein_id="HFO6463181.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(32126..32224)
                     /gene="ilvL"
                     /locus_tag="ACHKTW_002159"
     CDS             complement(32126..32224)
                     /gene="ilvL"
                     /locus_tag="ACHKTW_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HFO6463182.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
BASE COUNT         7802 a         8895 c         8208 g         7411 t
ORIGIN      
        1 gtcacaactt ctaataatgg ggtggctaat gggattcgaa cccacgacaa ctggaatcac
       61 aatccagggc tctaccaact gagctatagc caccactaca aatcttgtta cgcggtatta
      121 ctaccaccgc agctcaagcg ccgggactaa atggcgcgcc cgacaggatt cgaacctgag
      181 acctctgcct ccggagggca gcgctctatc cagctgagct acgggcgctt agcgccgttg
      241 cgggggtgga taatacggac ttcccacccc tctgtctagt ccctttttaa ataaattgcg
      301 cgtttggtta cggtttgtgc gttttgctgc ttattcctcc agtttatgcg ctttcccgtg
      361 gcgattaagg ccaaaaactt tataaatcgc cgtcaccgcc agcataaaga tgatgccaac
      421 aaacagcgac atacgcgtat cttcattaaa gtacatgccg atcaaaacgc aaatcaggaa
      481 tgccattgtt acgtagttgg cccacgggaa caggattgag cggaacggat ggctggcaat
      541 cgccgcttta tgtgcacgcc gaaaacgcag ctggcttatc aatatcacaa accatggcac
      601 catccccgga agcacgctgg cactgtagac gtagacaaac acacgctgcg gattgggaat
      661 gatgtagttc aggcatgagc caattagcag aatagcaata gatactgcca cacccgcaac
      721 tggtacgccg tgacgggaaa ctttcgccat cgccgccggt aactgacggt ttttcgccag
      781 tgcgtagagc atacgtccgc aactgtacat gccgctgtta cagccagaga gcgcagctgt
      841 cagcaccaca aagttgataa tgcccgccgc tgcggtaata ccgattttgg caaaagtcag
      901 tacgaacggg ctgccgttgc tgccaatttc attccacggg aagatggtga cgataacgaa
      961 aatcgcgcct acgtagaaaa tcaggatccg ccacagcact ttgcctacgg cactgcgcag
     1021 cgtcacctgc ggattcttcg cttcaccggc agtaatgcca atcagctcca cgccctggta
     1081 ggacgccacc acaatacaca gcgcggtcag gaaccctttc cagccacccg caaagaaacc
     1141 gccatgctct gtgagattgc taaaaccaat cgactgcccg ccattgccaa agccaaagaa
     1201 aatcacgccc aggccaatga caatcatcac gataatcgtg gtgactttga tcatcgcgaa
     1261 ccagaactcg atttcgccgt acaaccgcac cgccgccaga ttcgccaacg ccaccagcgc
     1321 cactgcgatc aatgcgggta tccactgcgc catctccggg aaccagaact ggacataaac
     1381 gccaatggcg gtgatttctg agatccccac ggccatccac ataaaccagt aagaccaggc
     1441 ggtgagataa ccaaagaacg ggctcatata acgatgcgca taaacggcga acgaaccggt
     1501 aaccggttcg aggaacaaca tttcgcccat tgaacgcatg atgaaaaaga cgaatagccc
     1561 ggcgatgata taggccaaca atacggatgg cccggcccat ttcaaggtac tggcggcccc
     1621 cataaacagg ccgacgccaa tggtgccccc cagggcgatg agttcgatat gtcgagcttc
     1681 cagcccacgc tgtagctctg gtttgttatc tgccataaat cctcgtgttg tgtttgcatg
     1741 ctttccggtg ttaccggtta tcgttatggg tacatcgagt gttgcgaatg ttttcgtaat
     1801 tcaggagaaa tggcaaataa agcattaaaa atttgaatgc tttgtgtaat aaaaaagcag
     1861 acaggcgacg gagtgaccac tccgtcgctt tacaaagaga ggaaaatcag aggttgccgg
     1921 tgtagtgcca gcgtaaataa cgcagcaaac gaagctgacg cttaatgcgg ctcggctgcg
     1981 aaagcaggcg gtagagccac tccagtccca gcgtttgcca gattttcggt gcgcgtttta
     2041 cgtgaccggt gaaaacatcg taagtcccgc caacgcccat atacagcgca tctggatgca
     2101 ccagacggca gtcgcgcatg aagatctcct gctttggcga tcccatcgca acggtgacga
     2161 tttgcgctcc gctggcatga atgcgttcaa acagcgcctg acgctgctcg ggtttaaaat
     2221 aaccatcctg actgccaacg atattcacat tccattggtt gcgcagttta gcttcagttt
     2281 gcgccagcac ttcaggttta ccgcccacaa gaaataccgg cgtcccttct ttgcctgcgc
     2341 gcgccatcag ctcttcccag agatcggcac cggcaacgcg ggaaacctgc gcctgcgggt
     2401 actttttacg tactgaacgt acaacgctga tgccatccgc atatttaaat tcggcagcgt
     2461 taattaactc cctgacctcg gcgttatctt caatggtcag cattttttcg gcattaatgg
     2521 caaccagcgt tccctgctta agctgcccgt cagcaaacag ataatcgagg gcgtgctgca
     2581 tatcacgcca accaatcaac tgtaagccac gcagcgtata ggtcggtgcc gtggtgttgt
     2641 tattcattgt tatccttcaa cctgcgtccg gagcgatgat tttgtacgtt tatgaatgag
     2701 tccggcgctt tcaaaaagcc agtacaacag ttttgcgatc atcagacatg cgccgaagac
     2761 cacgataaaa aagactacgc gtgagacaaa cgaatccagc ccttcacgtg ccagcacgat
     2821 catattgaag atcgccccaa agcagaaact gtgcaatatc gcagccttat agcgattagg
     2881 ctcgcggttg cccagctcat acagccagtc gaaccatttg atgatcagac caaccacgat
     2941 cgccccgagc gggatgaaca acgcgccgcc catcaccacc agtgagccta taagcgtagg
     3001 cgagatcgcc agtccggagt ggttattcag cacttcccag gtaaagtagt tggctgagtt
     3061 cagcaccata ctcgggcgac ccggccacag ccaggaaggg ataaagacat agaaatcgcg
     3121 gacaattgga gccaggccct ggaagtcgat gttgtcgtag ttctgcaaca gcaacgccag
     3181 attctcccac ggcgagaagg tgtcgcgagt gagatagaga aacgtataga acgcttcatc
     3241 gccgctcaca ttcattccat agcgttttag cgccagccag aacatgccca caatccccag
     3301 cacgcccgcc gccgccagca tccacaacga aatccagccg cgaataatgc caataaacag
     3361 gaagatagcg aatgcgatga tgatattggc gcgagtgccg ccgacaatca tataagtcag
     3421 caagccaaag gcgaccgtgc tgacgaggaa aaacagccac gctttgctat cctggcgcag
     3481 aaagtagacc actagcatcg ccgggatgaa aaagtaaaag aagcgtttta acgccacgcc
     3541 ggagacttca ctggagaaga tctgactgta ggagttaagc cggaacagca aaaagccgtt
     3601 gtgcatgaag aagatgccga cgcttaccag cgcgatgccc atcaggatca cccacgtaag
     3661 attggtctcc acgcggttca tggtaaacag cggacggcgc ggtacatcag caacgcgttt
     3721 gcgtaggcgg gttttgtagg tgacatagta aaccgcgtag aagcagcccg cagaaagcaa
     3781 cgcctgcaac aagatttctg gcggcgcgac accaacatca aagcgaaata ccagcacgct
     3841 ggtcagcggg aagccgaaga aaaaggtgag caaaaacagc aatgaaaaga agacattgaa
     3901 gttaaagcgc acacggcgaa actcaaacca ggtcagcgtg gcaataaaca gcgtgcagag
     3961 cagccagacc acaaacaggc cactgaattg cagcagactc atgcgccctc cccggcggca
     4021 atcgccaacg cccgctgcca gccttgtaga tagttagggc taaagaaggc aatggtgttt
     4081 ttatccaccg acgccaactg gcgctgcgct tcacgcacaa tatcctcgtt gagatcgtcg
     4141 gtagtaaaca gcactggcaa atgctgttcc gtcatatcct gccagaacgg attttcccgg
     4201 ttaagcacac aaggaatgcc cgcctgaatc agtaagcaca gcgtaccaat gccctgctgg
     4261 cgagcaaaaa taaagtaacc aagatcgcac tgacgaagta gcgccagata ggcgtcaaat
     4321 tccagttttt cgctcaaaat ttgcagattt tcttcgctga ataactccag ccccgcctga
     4381 cgaacttcct caatgtacgc ttcgttatta ggcggatatc ccatcggcac caccactttt
     4441 accgtatcgc caaattgctg atgaacggca cgtaaagcgg cgacatgctc attgctgagg
     4501 tcgccggagt tccccaccag aatggtcatt tttccttcac gttgccgatc gttcgccatc
     4561 gtattgagag aagggtccat ccgcgtcggg aagtacagca gttctccccg cacctttggg
     4621 tgcgttttgg caaaaaagct caaatcgccg cgggtggcaa atacacagcc gactcgcttt
     4681 tgcgccaggc gacgtagtgg gtaaaaaagc ttatatctca agccactgga aagctcatac
     4741 aggtctgccc cccagatatg ccagaaaaac tggctgggct taatgccacc actcagcaga
     4801 gccagccaca gtgtgggatt gaactgaccg tggaaaaaaa atcgctgctg acggttagct
     4861 tttgctttcg cgatgaccgc ttccgccagc gattttttcc cagggaaaaa ttgcacagaa
     4921 agcgccggac agctatcact taagccgtcg tccttgccaa caaccataaa ctcgcgcgcg
     4981 tgctcgctcg tcgcagccag cgcgtcattg aaaaaccgca aaacggttcg gttatggtga
     5041 gggatatccg atcccagtac gtgaatcagt acagtcatgc ccgcctacgc cagagtaaaa
     5101 acacgccaca acaaagagaa aaatagacga tataagttgc catatatgcc tgcgccgcgc
     5161 ccagtgcacc atgcgcaggg attagccagt gggcaaatac catcaataaa gtgaactggc
     5221 tgatttccgc cagaatataa aaccgcaatg acgctttggc gatcaccaga taaccaaaga
     5281 cataagcgcc cacttttaac acatcaccca ctaactgcca ggcaaagaga tcgcgcatag
     5341 cggtaaattt attcgacaac agcagccaga tagcaaaatc acgcagcagc cagacggtaa
     5401 aactcgccgc cgccactgcc ggtaagacga atttcagcga tttaaccact tcccgggtga
     5461 tatcgcgctt ttccgttagc cgcgacaacg tgggcagcaa atatacgctg aacgatgccg
     5521 taataaattg caggtaggca tcggaaatac tgctcacccc ttgccagatc cccacttcat
     5581 cccagctata ctgcgccgcc agcagtttac gcatcatgat gtaagcaaca ggcaaggtca
     5641 ccgacgtaat caacgccatg agcgtaaatt tgctcaactg ccctgccaga ccgttatccc
     5701 agctgggttt cagatagctt aacgggatga caccacgttt gatcaacatg atagcggcag
     5761 gaattaccac cagcgcggga atcagcgcca gacccagcaa cgccccttca taaccgccca
     5821 aacggtaact gacgtagtac gcgagaacgc caatcaagct gccgacaatc agcgataacg
     5881 cattacctgc ggcatcgcga aagcctttca tcagcgccag taacaggttg ccccaggcga
     5941 tccccatttg caccagcgcc actaaacgca ccagcccctg atagtcggta taaccaaaca
     6001 atccctggct gattggcgca gctgccagca caaaaaccag cgccatcagc gtagagaaac
     6061 caagtaccat cgctgatgaa gtgccgacca cgcggcgcag ctgttgcgga ttatcatggt
     6121 actgggcaac gtatttggtt acaccgttaa agatgccagc tccggcaagc acgccgagca
     6181 cggtaatcaa ctggcggaaa tttgccgcca gcccaagccc cgccggacca aatgacacag
     6241 ccagcaactt accgaccagt aacccggcac caatcttgac cagtgtactg gccgccgtcc
     6301 acaaggacgc ttttgccaac gacatatcag gagaagtagt tcaacaaagt cgcaattacc
     6361 gtacgctgat tgacgggcga caggttgtag aacagcggca ggcgcagcag gcgctcgctc
     6421 tctttggtgg tgtagcgatc ttcaccgtgg aactcaccaa agcgttcccc cgcagggcaa
     6481 ccgtgcagcg gaatgtaatg gaacaccgcc atgatttccg cttctttcag aaagttaatc
     6541 aacgcgctcc ggtcatcaat atcccgcagt ttaatgtaga acatatgcgc gttctgcacg
     6601 cagccatcgg gaatcgacgg cagctcgata cgcccggctt tcgccagagg cgctaacgca
     6661 tcgtagtagt tttgccacag cgccagacgt tgctggttga tacgatccgc tgcttccagt
     6721 tgcgcccaca ggtacgcagc ttgcagatcg gacatcaaat agctggagcc aatatcgcgc
     6781 caggtatatt tatcgacctg accacggaag aactggctgc ggttagtgcc cttttcacgg
     6841 atgatctcgg ctcgttcgat taacgcttta tcgttaatca gcgtcgcgcc gccttcacca
     6901 cccgccgtat agtttttggt ttcatggaag ctaaagcagc caatatgacc aatggttccc
     6961 agtgcacgcc ctttgtaagt ggacatcacg ccctgagcgg catcttctac cacaaacaga
     7021 ttatgctttt tcgccaacgc cataatggtg tccatttcgc aggccacacc cgcgtaatgg
     7081 accggcacga taacgcgcgt tttgtcggtg atcgccgctt caatcagcgt ttcgtcgatg
     7141 ttcatggtgt ccgggcgaac atccacaaaa acgatttttg cgccacgcag cacaaaggca
     7201 ttggcggtgg agacaaaggt gtagctcggc atgatcactt catcgccagg ctggatatcg
     7261 agcagcagcg ccgccatctc cagcgaagcg gtgcaggacg gcgtcagtaa cactttggcg
     7321 ctgccaaaac gttgctccag ccactgctgg cagcgacggg taaaaccgcc atcgccacac
     7381 agtttgccgc tacccattgc cgactgcata tagtcgagtt cggttcccac caccggcggt
     7441 gcgttaaatg gaatcatgtg atcacctgta taaccagtac gcggtgcttt ctacattcgc
     7501 accactttgt atgtatcgtt taagcgcggc ggtgttgccc atttgggtcg ccacccgcaa
     7561 agttgtttta ccgcgagcat acgcccagtt tagcgccgtt tgcatcagct cagcacctgc
     7621 accgcgtcca gccagcaggc caattcgcgc atctgtcgca ttgagttccc gtaaagagac
     7681 ataaccgcga ataccgccgg acgccgcacg taaaatcaga cattgatgat caaaggtgcc
     7741 gcgcacggca ttttcaatcc actgtgcata aaagcgtccg ctggcgtcag gcgcatacca
     7801 cggcgcacga aaacggcttt gcgcaaatgc ggcgctggct aactgacgta atgcgggaat
     7861 atcggtctct tgtgccacta cagcaccact atcactgaca ttgttcacgg gtagtgccaa
     7921 atcaacttca ccttctacca gggagaatcc cagctgttgc agggcatcca gttcacccgt
     7981 atttgatgcc gcaattttgg cctgcacccg tgaccacggc gctaacacgt ctggcgtcag
     8041 gagcggtgct tcagacgtaa tgcgcacgat ggcgctgtta acaccaaaga aggcgttttc
     8101 ccaggttagt ggctcaatac tggcgcggac gggcacgaag taactccagc agatattggc
     8161 cgtagccagt tttcgctaat gaactggcag cacgcttcac accctcgtca tcgagccagc
     8221 cgttacgcca ggcgatctct tccaggcagg caatcttaaa gccctggcgt ttttctactg
     8281 tctgcacaaa ggtactggct tcaatcaggc tgtcgtgagt gccagtgtcc agccaggcaa
     8341 atccgcgccc gagcagttca acggtcaggt tgcccgcctc gaggtacatc tggttgatgg
     8401 aggtaatctc cagttcacca cgctccgacg gcttcacctg ctttgcgtac tccacgactt
     8461 tactgtcgta gaaataaagc ccggtcaccg cccagtttga cttcggctgt tttggctttt
     8521 cttccagcga gatagcgcgg aaattgtcgt caaactccac cacgccaaag cgttccgggt
     8581 ccatcacctg atagccaaat accgtcgccc cttccgtgcg cgccgcaaca tgacgcagct
     8641 tcggactgaa gccctgaccg aagaagatgt tatcgcccag caccagacaa gaaggttcac
     8701 cgttgaggaa ggtttcaccg atgataaagg cctgcgccag accgtccggg ctgggctgtt
     8761 cggcgtattc cagctggata ccgaactcac taccatcgcc cagcaggcgc tggaaataac
     8821 ctttatcttc cggcgtagtg atgatgagaa tttcgcggat accggccaac atcagcaccg
     8881 acagcggata gtaaatcatt ggcttatcgt aaatcggcaa cagttgcttc gatacgccgc
     8941 gcgtaatcgg atgcaatcgg gtgccggaac cgcccgccag gataatacct ttcatgtttg
     9001 cctccgctga aaattagcct ttcagaccta aacgctcgcc ctgatagctg ccgtcctgca
     9061 cctgcttcca ccagctttca ttagccagat accactgcac cgttttacgc attccacttt
     9121 caaaggtttc ctgtggcagc caaccaagtt cacgggcaat tttcgaagca tcaatggcat
     9181 aacgcagatc atgccccgga cggtcagcga caaaggtgat caagtcacga tagtgcgcca
     9241 cgccgtgcgg cttgttcgga gccagttctt ccagcagctc gcaaatggtt tccacaacat
     9301 cgagattctt acgctcgttg tgaccaccaa tattataggt ttcaccgact ttcccggtgg
     9361 tcgccacgca atacagcgcg cgggcgtgat cttccacata cagccagtca cggatttgct
     9421 gcccgttgcc atataccggc agcggtttac ccgccagcgc gttgaggatc atcagcggga
     9481 tcagtttttc cggaaagtgg taaggaccgt agttattcga gcagttggtg atcagcgtcg
     9541 gcagaccgta ggtccgcagc caggcgcgca ccagatggtc gctactggct tttgacgcgg
     9601 aataagggct gctcggcgca tacggcgtgg tttcggtgaa gaaatcatcc gtcgagtgca
     9661 ggtcaccata tacttcgtcg gtggagatat gatgaaaacg gaacgctgat tttttatctt
     9721 ccgtcagcgt attccagtaa gcccgcgccg cttcaagcaa tgtataagtc ccgacaatgt
     9781 tggtttcaat aaacgctgcc gggccgtcaa tagaacggtc aacatggctt tcggctgcca
     9841 gatgcatgac acagtctggc tgatgctcag tgaatacgcg tgccagttct gcccgatcgc
     9901 agatatcaac tttctcaaag gcaaagtgct cgctttgcgc gaccggtgcc agcgacatca
     9961 ggtttccggc gtaggtcagc ttatcgacca ctaccaccgc atcgctcgtt tcgttgatga
    10021 tataacgcac cagcgccgag ccaataaacc cggcaccacc tgttatcaga atttttctca
    10081 tcagcgccag actcctttgg catcgacgac atactgctga tggacattgt cgccattgat
    10141 aactttgaac tgactatgat cgaccagcat caccagcacg tctgccgttg ccagcgcctc
    10201 gtcaagctgc gccagagtac aaagcccggt cagttttttc ggcaactggt ggatgttagg
    10261 ctcaacaacc agcgtttcgc cgctgtgcca ctgggcgatc agttcagcga tttccatcgc
    10321 cgggctttcg cgcaggtcat caatattcgg tttaaacgcc agaccaaagc aggcgatttt
    10381 cagttcactg gcgcgtttat cggtagcagc aaggcagtcg gcaaccgccg ctttcacctg
    10441 atcgataacc cagaacggtt tgtgatcgtt aacttcgcgc gcggtacgga taagccgcgc
    10501 ctgctggggg ttctgtgcca cgataaacca cggatcaaca gcaatgcagt gaccgcccac
    10561 gccagggcca ggctgaagaa tattaacgcg agggtgacga ttcgccaggc gaatcagttc
    10621 ccagacgtta atcccctgat cggcacaaat cagcgacaat tcattagcaa aagcgatatt
    10681 cacatcgcgg aagctgtttt cggtgagctt acacatttcc gccgtccgcg agttagtgac
    10741 gacacactca ccttcgagga aaattttgta cagttcgctg gcgcgggtcg aacaaaccgg
    10801 cgtcatacca ccaatcacgc gatcgttttt aatcagctcg accataacct ggcccggtaa
    10861 cacgcgttcc gggcagtaag caatgttgac gtccgcctgc tcgcccacct gctgtgggaa
    10921 agtgagatcc ggacgcatct ctgctaacca ttctgccatc ttctcggttg accccaccgg
    10981 cgaggtggat tcaaggatca ccagcgcgcc ttttttcagc actggcgcaa tggagcgagc
    11041 agccgattca acgtaggtca tatctggctc atgatcgcct ttaaacggcg tgggtacagc
    11101 aatcagccag gcatccgctt caactggcgt cgtgctcgct cgtaaaaaac cgccttctac
    11161 ggcagttttt actacgctcg ccaaatcagg ctcgacgata tggatttcgc cacgattgat
    11221 ggtatcaacc gcgtgttggt tgatatcgac accaatcacc tgtttttgcc gtgaggcaaa
    11281 cgctgctgct gtaggcagcc cgatgtaacc cagtccgata acagaaatgg tcgcaaaact
    11341 catagtgata tccgattatt ttttaacgct tccagaatgc gagagcatgc ctgaccatca
    11401 ccatacgggt tatgggcgcg gctcatagtt tgatattcgt tttcgtcttt taaaagacgc
    11461 gtcacttcct cgacaattcg ctgcttatcc gtgcctacca gacgcaccgt acccgccgtc
    11521 accgcttccg gacgctcagt ggtatcgcgc atcaccagca ccggtttccc cagcgaaggc
    11581 gcttcttcct gaatgccgcc tgagtcggtc aaaatcagcc aggcgtggtt catcagccag
    11641 acaaacggta aatactcctg gggatcgatc agaatgacat ttttcacatg ccccagaatg
    11701 cgattgaccg gctctctgac gttcgggttg agatgcaccg gatagacaat ctggatgtcc
    11761 tggtgcgtgg tggcgatgtc tgccagcgcg tggcagattt cttcaaagcc acgaccgaaa
    11821 ctttcacgcc tgtgaccggt caccagaatc atctttttat cggggtcgat aaacgggtaa
    11881 tttgccgcca gttctgaacg cagcgtgtcg ctgctcatca cctgatcacg cacccataac
    11941 agtgcatcaa tgactgtatt accagtaatg aagattcggc tatccgcaac gttttcacgc
    12001 agcaagtttt gccgggaagt ttcggttgga gagaagtgat acatcgccag atgcccggtc
    12061 aatgtacggt tagcctcttc cggccacggc gaatagagat cgcccgtgcg taaaccagcc
    12121 tcaacgtgac caacaggaat acgctgataa aacgccgcca ggctggttgc cagcgtcgtt
    12181 gttgtgtcgc cgtgaaccag cacgacgtct ggtttgaact cggcaagaat aggttttagc
    12241 ccttccagaa tccgacaggt tatctctgtc aggccctgtc ctggctgcat tatgttgaga
    12301 tcgtagtcag gtacaatgga aaagagtttc agcacctgat cgagcatctc ccgatgctgc
    12361 gcagtgacgc aaactttagc ctcaaaaaaa ggatcttttg ccaacgcatg caccaacgga
    12421 gccatcttga tggcttccgg gcgcgtacca aatacagtca gtactttcac atcgattctc
    12481 ttcgaataag cggcgagcgc ctttgcgctc gcagcagcag tgttgctatt tcgagcaacg
    12541 gcgggttaat gcgacaccag ccccgatcag ccccccgaca atgccccaca taatcatcag
    12601 gaaggcacga cgtgggctat cgcgttttac cggttcttcc ggcgtacgca aatagcgata
    12661 ggtctgaaaa cgcggatcca gagttggacc aacattcagg gtgtttaaca tggcccgatt
    12721 ctgatcatag tcgagatcaa aggccggacc gacggcctgt aaattttcca gtcgagcctg
    12781 gagcattgga cgcccaagca ggaacatttc tgaatcaggt aattcctcgg caggcacatc
    12841 tgtcgcactg cgcgaaatat tatgctgctc agcaattttc agcgcctgct caatgctgtt
    12901 catccggcgg tcgtagatgg ctttcgccac ctcttcctga cgcttcacct gagctttcat
    12961 ctggatggtg cgtgccgccc atgcgccttt cagctcatca ttcagatggc tggctgcacg
    13021 ctggctggca aaagcaacat actgacgtaa caggttatta gcgtcaggcg cggtttcggc
    13081 aataagcttc acgctgtcat tgaccgcgcg ggtaaagtct ccggggataa actggatgtt
    13141 gttaatcatt tcatccagca acgccgcatc ggctttgctg ttgcccacca tccgctgttt
    13201 gtaatagtcg gtttgcagcc agaactctct gcgggtatcc cacgaggcca gttgcataac
    13261 aaactcttta taggcttcgt ccatgaccga tggttgatcg gcagaagcca tgtttgaacg
    13321 gacatccagg ttacgcaaaa attgctgctg cgagtaatat ccccccagca tattcaccgt
    13381 tggacgatcg gtaatcgccg tcgagctcca ctcctgacga gcaaaaaaag tatacgccag
    13441 cgcgattaac gcaaacgcca gccccatgcc aataatccat agcttcccag cccacaaggt
    13501 acgaaacaac ccacgaatat ccagttcatt ttcagcgtct tcggccggtt tcccaggcat
    13561 tggttgtgtc atcacatcct catttatttg gttaaattgg ggctgccacc acgatttcta
    13621 cgcagtctgc gttttacgcg cttaataaag cgagcaactt tccaggcacg cttaatgcaa
    13681 tatccataga ggaagaatgc tagcaaaaag agcaccagca tgacccactc cgggacaaaa
    13741 tgagaatatt ctgccagcac gccaatggaa gcgagcagtg ctgcggcaag ggtaatcagc
    13801 acaaacgcct gacgggaagt aaacccggca cgcatgatca aatggtgaat atgctgacgg
    13861 tcaggagaga atgggctcat gcctttacgc aggcgacggt acataatcgc caccatatcc
    13921 attagcggaa tggcgattat ccacaaagcg gtaaccgggc tgatgggatg ggttttgccc
    13981 tgggtcgttt cgagcaggat ccagataacg gtaaaaccaa tcagcgtact gcccgcatca
    14041 cccataaaga ctttgtagcg gcgacccagg ataccaaggt taagcatgat gtatggcagg
    14101 atggcggcga tcatcgcaaa gcaccagatt gcgaggctgg tttgcccgtc gaaccacaaa
    14161 atcataccga ttgctgcaaa cgagacgcag gacaacccgc ccagcaagcc atcaatgcca
    14221 tcaaccatgt tgaacgcatt aatggccgcc cagacggcaa atagcgtcag gaagtaacca
    14281 aacggtccga gcaccatctc ccaggagcca aagatataac ccaggctact gagataaagt
    14341 ttgccgaaca ccatcataac aatgccaaca gcggcctgta tggtggcacg gatttttacg
    14401 ctgatatcaa aacggtcatc cagcgcgcca atgaaaacaa gcacaccggc acaagcgaga
    14461 tagagagatg catgcggaat atagtaatcg acaattccga acgtgaagca aatccctgcg
    14521 taaaccgaaa tccccccaac gagaggtatc aatccctggt gacgtttgcg gaagtttggt
    14581 ttatccacta aaccgacttt ttttgccact ttacgggcaa aaaacagaaa cagtgtcgtg
    14641 aataaaaaaa tactgatgag atcagtactc actgtcagta aattcacaat gcatgctctc
    14701 agagaaaatt attagcagaa gtataaccac gaagaccttt attcagaagg gaaagtccgt
    14761 tccgaaccca ccagtcctgt gcaaaaatta accatttgat atgcaaattg cataacatgc
    14821 cagcaaattc caggaatttt cctaacatcg ccagcgcggc attaagatta cagatataaa
    14881 agcaaaacgc cacgtaaaca cgtggcgttt ttggcataag acaaatttat gagcgtttca
    14941 tcatttcgaa gaaatcgtca ttggtcttag tcatggccag tttattaatg aggaattcca
    15001 ttgcatcgat ttcgcccatc gggtgaatga ttttgcgcag gatccacatt ttctgcagtt
    15061 cttcctgagt cgtgagcagc tcttctttac gggtaccgga acggttgtag tcgatagccg
    15121 ggaagacgcg tttttcagcg atcttacgag agaggtgcag ttccatgttg cctgtacctt
    15181 taaactcttc gtaaataact tcgtccattt tagaaccggt atcgataagc gccgtcgcga
    15241 taatggtcag gctgccgccc tcttccacgt tacgcgccgc accgaagaag cgtttcggac
    15301 gatgaagggc attggcgtcc acaccaccgg tcaacacttt acctgacgcc ggaacaacag
    15361 tgttgtaagc gcgcgccaga cgagtgatgg agtcgagcag aatgataacg tctttcttgt
    15421 gctcaaccag gcgtttcgcc ttctcgatca ccatttccgc aacctgaacg tggcgagatg
    15481 cgggttcgtc aaaggtagaa gcaacaactt caccttttac cagacgctgc atctcggtta
    15541 cttcttccgg acgttcgtcg atcagcagaa ccatcagcac acaatccggg tggttgtaag
    15601 caatgctctg agcaatgttc tgcagcagca tggttttacc ggctttcggc ggtgccacaa
    15661 tcagaccacg ctgaccacga ccgataggtg atgccagatc cagtacgcga gcggttaaat
    15721 cttcagtaga accgttacca cgttccatac gcagacgaga gtttgcgtgc agcggggtta
    15781 agttctcaaa gaggattttg ttgcgggcgt tttcaggttt gtcgaagtta acttcgttaa
    15841 ctttcagcag cgcaaaatag cgttcacctt ctttcggcgg gcgaatctta ccagagatgg
    15901 tatcaccagt gcggaggttg aaacggcgga tttggctagg ggaaacgtag atgtcatcag
    15961 gaccggcgag gtaggagctg tctgcggaac ggaggaaacc aaatccatcc tgcaatatct
    16021 ccagtacgcc atcaccaaag atatcttcgc cactctttgc gtgctgcttc aggatggcaa
    16081 aaataatgtc ctgcttacgc atacgagcca ggttttccag ccccatattt tcgccgagag
    16141 tgatcagctc agaaaccggc gtattcttta attcggtaag attcatagtg gtgtgagttc
    16201 ttaaacttgg ggtaaatctc gaacttaatg ttgtgaatgg tatggcagga tcatccatgc
    16261 ctgtttacgg tcatcgactc atgtcttttc gctgcctggt cacaggagag tacgcagaac
    16321 tgaaacgaca agacggattg agtgacgagc cagaaatctg ttcacttcgc atttaagata
    16381 aaacgggaag cgttggaaat tacaagattc aaacttaata aggtatgttt aatacgaagt
    16441 caacactaag ttagcatgac tcacgccggg cgtccagttt ttagcgacgg ggcacccgaa
    16501 catgaaattc ccttacgcca ggttagcgtc gaggaactct ttcaactgac ctttagacag
    16561 tgcgcccact ttggttgccg ccacttcacc gtttttgaac agcagcagag tcgggatacc
    16621 acggatgcca tatttcggcg cagtgccagg gttttgatcg atgttcagtt ttgcaacggt
    16681 cagtttgccc tgatattcgt cagcgatttc atccagaatc ggggcgatca ttttgcacgg
    16741 accgcaccac tctgcccaga aatcgacgag gatcgccccg tccgctttga gtacatccgt
    16801 gtcaaaactg tcgtcagtca ggtgaataat tttatcgctc atatataact ccacaggaat
    16861 aagcctggcg tgttggtttc gttgttggtg taacattaac caactaaagg ttgactttat
    16921 ttcaccggat acgctttcgt aaagcaatag taagctgata ttctaccaca ctatgagcaa
    16981 aacacattta acagaacaga agttttccga cttcgccctg catccgaagg ttgtagaagc
    17041 ccttgaaaaa aaagggtttc ataactgtac gcccattcag gcactggccc ttccgctgac
    17101 gctggcgggt cgtgacgtag ccgggcaggc gcaaaccggt accgggaaaa cgatggcgtt
    17161 tcttacgtca acgtttcatt atcttctctc tcatcctgcg attgccgatc gcaaggtgaa
    17221 tcaaccacgt gccttaatta tggcaccgac gcgtgaactt gccgtgcaga tccatgccga
    17281 cgcagaaccg ctggcggaag ctaccggcct gaagctgggt ctggcttacg gtggtgatgg
    17341 ctacgacaaa cagctgaaag tgctggaaag cggcgttgac attctgattg gcactacggg
    17401 gcgtttaatt gactacgcca agcagaacca cattaacctc ggtgccattc aggtggtggt
    17461 actggacgaa gccgatcgca tgtacgatct gggctttatt aaagatatcc gctggctgtt
    17521 ccgccgtatg ccgcctgcaa accagcgcct caacatgctg ttctccgcca cgctttcgta
    17581 ccgggtacgt gaactggcgt tcgagcagat gaacaatgcc gaatatattg aagtggaacc
    17641 ggaacagaaa acgggccacc gtattaaaga agagcttttc tacccttcta acgaagaaaa
    17701 aatgcgtttg ctgcaaacgc tgatcgaaga agagtggcca gacagagcga ttattttcgc
    17761 caataccaaa caccgttgtg aagagatctg gggccacctg gcggcagatg gtcatcgtgt
    17821 cggtttgttg acaggcgatg tcgcgcagaa aaaacgtctg cgtattcttg atgaatttac
    17881 ccgtggcgat ctggatattc tggttgccac cgacgttgcc gcgcgtggtt tgcatattcc
    17941 ggcagtgacg cacgtcttta actacgattt acccgatgac tgtgaagatt acgttcaccg
    18001 tattggtcgt acaggtcgcg caggcgcaag cggtcactct atcagcctgg cgtgtgaaga
    18061 gtatgcattg aatttgcctg ctattgagac ctatattggt cactcaattc cggtaagcaa
    18121 atacaatccg gacgcattga tgaccgatct gccaaaaccg ctgcgcctca cgcgcccacg
    18181 tacaggcaat ggcccgcgtc gtactggcgc tccgcgtaat cgtcgtcgtt caggttaaat
    18241 aaaatgtcat actcaaaatc attcaaactg tatcaagatg gcaagagaat gaatcccgat
    18301 gagcatactg tagtaagtga ttcaggtgaa agaatgcagc caacacagag acagattgaa
    18361 ggatgaagag tatgggttcc acctcgtcgc tgtatgcagc cattgatctc ggttcgaata
    18421 gttttcatat gctggttgtg cgcgaggtgg ctggaagcat ccagacgctg acgcgaataa
    18481 aacgcaaagt gcgtctggct gctggcctga atagcgaaaa tgccctgtct aatgaagcaa
    18541 tggagcgcgg ttggcaatgt ctgcgcctgt ttgctgaacg tctgcaagat atccctccct
    18601 cgcaaattcg cgttgtcgct acggcgacat tacgtcttgc cgtcaatgcg ggtgatttta
    18661 ttgccaaagc gcaggaaatc ctcggttgtc cggtacaggt gatcagcggt gaagaggaag
    18721 cacgtctgat ttatcagggc gttgctcaca caactggcgg tgccgatcag cgcctggtgg
    18781 tggatatagg cggtgccagt actgaactgg taaccggcac gggtgcacaa accacctcgt
    18841 tgttcagcct gtcgatgggc tgcgtcacct ggctggaacg ctattttgcc gatcgtaatc
    18901 tggggcagga aaattttgat gctgcagaaa aagcggcacg cgaagtgtta cgtccggttg
    18961 ccgatgaatt acggtatcac ggctggaaag tgtgcgttgg tgcttccggc accgtgcagg
    19021 cgttacagga aatcatgatg gcacagggga tggatgaacg cattaccctg gaaaagttgc
    19081 agcaattgaa acagcgagcc attcattgcg gtcggctgga ggaactggag attgacgggc
    19141 tgacgctgga acgtgcgtta gtgttcccga gtggtctggc gatcctgatc gccattttta
    19201 ccgaactgaa tattcagtgt atgaccctgg cgggcggtgc gctgcgtgaa ggcctggtct
    19261 acggcatgtt gcatctggcc gtcgagcagg atattcgcag ccgtacgctg cgtaatattc
    19321 agcgccgctt tatgatcgat attgatcagg cgcagcgcgt agccaaagtt gcggctaact
    19381 tcttcgatca ggtggaaaag gaatggcatc ttgaagcaat aagccgcgat ttgctcatca
    19441 gcgcctgtca gcttcatgaa atcggcctga gcgttgactt taaacaagcg ccgcaacacg
    19501 ctgcttatct ggtgcgtaat ctggatcttc ccggttttac ccccgcacag aaaaaattgc
    19561 tggcgacgct actgctcaac cagactaatc cggtcgatct ctcatcgctg catcagcaaa
    19621 atgccgtacc tccgcgcgtc gcagaacaac tctgccgttt actccgcctg gccatcattt
    19681 ttgccagccg tcgccgtgac gatctcgtgc cagagatgac attacaggct aaccatgaac
    19741 tgttgacctt gacgcttccg caaggttggc taacccaaca tccgctgggt aaagagatta
    19801 ttgctcagga aagccagtgg cagagctatg tccactggcc gctggaagtg cattaatgag
    19861 taagtgccgg atgcgatgct gacgcatctt ttccggcctt gattatttcc ctcgttttgc
    19921 cgccatcatc gctttcagat tcgccagatg gctttgccct ttctgcatcc gttcttcggc
    19981 gctgaccact ttgcgctcct gttcccaaat cagatcatcc tgcggcagct ccagcaaaaa
    20041 gcggctcggc tccgggcgca ccagttcgcc gtactgacgg cgttctttac acagcgtaaa
    20101 ggtcaattcc ttctgggcgc gggtaatgcc gacataggcc agccgccgct cctcatcgat
    20161 attatcttca tcgatactgc tctggtgcgg caaaaaccct tcttccatac cgaccatgta
    20221 gacataagga aactccagcc ctttcgacgc gtggagagtc atcagttgca cctgatccag
    20281 ctcttcttca ctctcaccac gctccatcat gtcgcgcaaa gtaaagcgcg tcaccacctg
    20341 ggtgagcgtc atcggctcat ccagttcgct accttccagc atctccgtca tccagctaaa
    20401 cagttggttg acgttcttca tgcgcatttc ggcggctttc gggctgggcg atgtttcgta
    20461 cagccaggat tcataatcca tgccgtggat cagatcgcgc accgcggcaa tcggctcccg
    20521 ctccgccaga cgctggattt ctgccaacca gtgagtaaag cgggtcaatg cttcataacc
    20581 acgtccgcta agcgtctgac tcaggcccat atcaaagctg gcggtaaaca tgcttttatt
    20641 gcgcgtcatc gcccactcac ccagcttttt cagcgtcgcc gggccaatct ctctcttcgg
    20701 cgtgttaacg atacgcagaa atgcgctgtc atcgtccggg ttagtcagca cgcgcagata
    20761 agccagcaag tccttgattt caggacgaga gaaaaacgac gtaccaccag atattttgta
    20821 tgggatgcgg ttttgcatca ggaatttttc aaacacccgc gactgatggt taccgcgata
    20881 aagaatggcg taatctttgt actgcgtttt attgacgaag tgatgggcga tcagctcgcc
    20941 agtaacgcgc tcggcttcat gttcctcgtt attcgcgctt aaaactttta gctctgtgcc
    21001 gtaacccagt tcggagaaca gacgcttttc aaagacgtgc ggattattgg cgatcaggat
    21061 gttcgccgct ttcaaaatac gtccggaaga gcggtagttc tgctcaagct taatcacctt
    21121 cagcgccgga aaatcctggc tcagcagcac caggttttgc ggacgtgcgc cgcgccagga
    21181 gtagatcgac tggtcatcgt cacccaccac ggtaaagcgc gcgcggctgc ccaccagcag
    21241 tttcaccagt tcatactggc tggtgttggt gtcctgatat tcgtccacca gcaggtagcg
    21301 aattttgttc tgccagcgct cgcggacttc ttcattgcgt tgcagaagta gcgtcggcaa
    21361 taaaatcaga tcatcgaagt cgagcacatt acaggctttc aggtgtgcat catacagccc
    21421 ataacaatgg gcaaaaatac ggtcccgctc gccaatcgca cttgctgccg cctgggacgg
    21481 tgttttgaga tcattcttcc agttagagat ggtcgaaatc agttgttgca ggagaacttt
    21541 gtcatcttca atcagcccct cggtcaactc tttaagcaaa gcaagctgat cggtatcgtc
    21601 aaacagcgaa aagttcgcct tcatcccaag cgccgcatac tcgcgtttga tgatatccaa
    21661 ccccagcgtg tgaaaagtgg agatcatcag cccgcgcgcc tctttgcgac ccagcgtctg
    21721 cccgacacgc tctttcatct cgcgcgccgc cttattggta aaggtcaccg ccgcgatatg
    21781 tcgcgcctga taaccgcaac cgcggatcag atgggcgatt ttattggtga tcacacgagt
    21841 tttaccggaa cccgcgcccg ccagcaccag gcaggggccg gtaacgaatt cgacagcttg
    21901 ttgttggccg gggtttagac gcataggtgt attgctcaat cttcgaacgg ggggaggatt
    21961 gtagcagaat cgcatttgaa tgtgaccgcg tgtaagccgc taaccggttg gctgcggatg
    22021 tttaaatcag agagagaaga ccgaaaccgg agttcatcgt gaatccatta gacttagaaa
    22081 atatcgggtc gtaacgttgt gttattactt tttcagagtt tactaaggag aggtgtactc
    22141 gtggcgatta atttcagtcc taaggttggt gaaatactgg aatgcaactt tggtaactat
    22201 cctgttagcc agaatggaca gttcagcacc aagtattacg atggccgcat tcctcctgaa
    22261 atgataaaaa acaggttagt tgtcgtgctc aacggaaaaa ttaatggcaa cgcatgtatc
    22321 gtcgttccat tatcaaccac tcgcgatcat gacaagttaa atcgtggaat gcatgttgaa
    22381 atagccagta atgttatcaa tggcctgcaa ttctttgatc agcagattcg ttgggccaaa
    22441 gccgacctgg tacaacaagt cagtcgaaac cgattaaata gagcaaggac ttacagaggg
    22501 tatcttaatc aatgtttgcc acatgagttg gttgcagaca tacagagggc ggtcatcaag
    22561 tcaatcaatg ccatatcgct cataaattga caataaaatc accatccccg ataatgactt
    22621 tgtagcccat agaggcccta agttttctcc ccttgaggga cctgcgaagc ccggattttc
    22681 cgggcttttg ccatatatgt accagcccct ccctgactga caattacgtt agcatctgcg
    22741 gatcatgcta aaatcgccgc ctgacaatta ttcactcaag gcacgatcat ggcaaaaaca
    22801 gcagcagcac tgcatatcct tgtaaaagaa gagaaactgg ctctggatct tctcgagcag
    22861 attaagaacg gtgccgattt cggcaagctg gcgaagaaac actccatttg cccatcaggc
    22921 aaacgcggcg gtgatttagg tgaattccgc cagggtcaga tggttccggc gttcgataaa
    22981 gttgtgttct cttgtccggt actggagcca accggcccgc tgcacaccca gttcggttac
    23041 cacatcatta aggtgctgta ccgtaactaa tagcaaggcc ttctccagga gaaggccttg
    23101 agtgttttct ccctctccct gtgggagagg gtcggggtga gggcatcagc gcgcacttaa
    23161 cccgcaactg caatacgttt catatccgtc atatagccgc gcagtttctt acctacctgc
    23221 tcaatcgcat ggcagcgaat cgcttcgttc acatcacgca gttgcgcgtt atctaccgca
    23281 ccttccggaa tagctttacc cagatcgccc ggttgcagct ctgccataaa cggtttcagc
    23341 agcggcacac aagcgtaaga gaacagatag ttaccgtact cggcggtatc agagataacc
    23401 acgttcattt cgtacagacg cttacgggcg atggtgttgg caatcagcgg cagttcgtgc
    23461 agtgattcat agtaagcaga ttcttcgatg atgccggaat caaccatggt ttcaaacgcc
    23521 agctcaacgc ccgctttcac catcgcgatc atcagtacgc ctttatcgaa gtactcctgc
    23581 tcgccgattt tgccttcata ctgcggcgcg gtttcgaagg cggttttgcc ggtctcttca
    23641 cgccaggtca gcagtttctt atcatcgttg gcccagtccg ccatcatacc ggaggagaat
    23701 tcgccggaga tgatgtcgtc catatgtttc tggaacaggg gtgccatgat ctctttcagc
    23761 tgttcagaca gcgcgtaagc acgcagtttc gccgggttag agagacggtc catcatcagg
    23821 gtgatgccgc cctgcttcag cgcttcggtg atggtttccc aaccgaactg aattagtttt
    23881 tctgcgtatg ccggatcggt gccctcttcc accagcttgt cgaagcacag cagcgagcca
    23941 gcctgcaaca taccgcacag gatggtttgc tcacccatca ggtcagattt cacttccgca
    24001 acgaaggacg attccagcac gcccgcacgg tgaccaccgg ttgctgccgc ccatgcttta
    24061 gcgatcgcca taccttcgcc tttcggatcg ttttccgggt gaacggcaat cagcgtcggt
    24121 acgccgaatc cacgtttgta ctcttcacgc acttcggtgc cagggcattt cggcgcaacc
    24181 atgactacgg tgatatcttt acggatctgc tcgcccactt cgacgatgtt gaaaccgtgc
    24241 gagtaaccca gcgccgcgcc gtctttcatc agtggctgta cggtgcgcac tacatcagag
    24301 tgctgcttgt ccggcgtcag gttaaccacc agatccgcct gcgggatcag ttcttcgtaa
    24361 gtaccaactt taaaaccgtt ttcggtcgct ttacgccagg acgcgcgctt ctcggcaatc
    24421 gcttctttac gcagagcgta ggagatatcg agaccagaat cacgcatgtt caggccctgg
    24481 ttcagcccct gtgcgccaca gccgacgata accacttttt taccctgaag gtagctcgcg
    24541 ccgtcggcga attcgtcgcg ccccataaag cgacatttgc ccagctgtgc cagctgctgg
    24601 cgcaggttca gtgtattgaa gtagttagcc atggtgattc ctcgtgatgt tgtgcttctt
    24661 attgtttggt tcgcgtttgc gagattgaat tcactatatg acaggaaatt tattgcggaa
    24721 attgatatat tcacaacgtc acattgcaat ttctgcaacg tcaacattga gggctgcgcc
    24781 attggattta cgcgatctga aaaccttcct gcatctggcg gaaagccgcc attttggccg
    24841 cagcgcgcgg gcgatgcacg ttagcccgtc cacgctctca cggcagattc agcgcctgga
    24901 agacgatctc ggtcaggcat tatttgtgcg cgataaccgg acggtgacgc tgaccgaagc
    24961 gggcgaagag ttacgcgttt tcgcccagca aacattgttg cagtatcagc aactgcgcca
    25021 caccatcgat cagcaagggc cgtcgctctc tggcgagtta catattttct gctcggtgac
    25081 cgccgcttac agccatctac cgccgattct cgaccgcttc cgcgcggaac acccgtcggt
    25141 ggagattaaa cttactactg gtgatgcggc agatgcgatg gaaaaggtgg tcactggtga
    25201 agcggatctg gcgattgcgg gtaaaccgga aaccttgccc ggcgcagtgg cgttttcgat
    25261 gctggagaat ctggcagtag tgctgattgc ccccgcgctg ccctgcccgg tgcgtaatca
    25321 ggtgtcggta gagaagccgg actggtcaac ggtgccgttt atcatggccg atcaggggcc
    25381 ggtacgccgc cgcattgaac tgtggtttcg gcgtaacaaa atcagtaacc cgatgattta
    25441 cgccacggtt ggcgggcatg aagcgatggt atcgatggtg gcgctcggct gtggcgtggc
    25501 gctgttgccg gaagtggtgc tggaaaacag cccggaaccg gtgcgtaacc gcgtgatgat
    25561 actagagcga agcgatgaga aaacgccgtt tgagctgggc gtgtgtgcgc agaaaaagcg
    25621 gctgcatgag ccgctgattg aagcgttctg gaagatttta ccgaaccact aacccgccaa
    25681 aaagaacctg aacgccgggt tattggtttc gtcgtggcaa tcgtagccca gttcattcag
    25741 ccgggtttcg aaatccggtt catggtcgcc aagctcgaac gccgccagta cgcgcccgta
    25801 gtcggtaccg tgactgcgat aatggaacag agaaatattc cagtgcgtac ccagcgtgtt
    25861 gaggaagcgc agcagcgcac ccggcgattc cggaaattcg aagctgtaga gacgttcctg
    25921 caacggatgc gatggacgcc cgccaaccat atagcgcaca tgtagcttcg ccatttcatc
    25981 gtcggagaga tcaaccacgc tgtagccgcc gtcgttgagc atctgcaaaa tttctttgcg
    26041 ctcttcgagg ccgcggctta agcgcacgcc gacaaagatg caggcgtttt tggcatcggc
    26101 aaagcggtag ttgaattcgg tgaccgaacg tccgccaagc aactggcaga atttgaggaa
    26161 gctacctttc tcttccggaa tggttaccgc cagcaacgct tcacgctgtt cacccagctc
    26221 acaacgttcc gagacgtagc gcaggccgtg aaagttcacg ttagcaccgg aaagaatatg
    26281 cgccagccgt tcgccgcgaa tgttgtgctg ggcgatatat tttttcattc ccgccagcgc
    26341 cagcgcgcca gagggttccg ccaccgcacg cacatcttcg aataaatcct tcatcgccgc
    26401 acagatcgca tcgctatcga cggtgatgat gtcgtcgaga tactcctggc ataaacggaa
    26461 ggtttcgtca ccgatgcgtt ttaccgctac gccttcagca aatagcccta cgcgcggcag
    26521 atcaaccgga tgacccgcat ccagcgctgc tttcaggcag gcggagtctt ccgcttctac
    26581 ggcgatcact ttgatttgtg gcatcagttg tttgatcagc accgcaacgc ccgccgccag
    26641 accgccgccg ccgactggca caaatacgcg gtcgagatgg gcgtcctgct ggagcagttc
    26701 cagcgccagc gtgccttgcc cggcaatcac catcggatgg tcgaacggcg gcacccaggt
    26761 gaacccctgc tgctgtgaca gttcgatcgc tttagctttc gcttcgtcaa agttcgcgcc
    26821 atgaagcagc acttcgccgc cgaatccacg taccgcatcg actttgatgt cggcggtggc
    26881 ggttggcata acgatcaggg ccttcacgcc taaccgcgca gaagaaaacg cgacgccctg
    26941 cgcgtggtta cccgcagaag cagtgatcac gccgtgcgct ttttgttctt ccgtcaggcc
    27001 cgccatcatg gcgtatgcac cgcgcagctt aaagctgtgc actggctggc gatcttcgcg
    27061 cttcaccaga atgacgttat caagacgcga cgacagtttt tccatttttt gtagcggcgt
    27121 aacctgcgcc gcctcgtaaa ccggcgcgcg cagcactgct cttaaatatt cggcaccttc
    27181 cggagcaccg gacaggggtt gcgagtcagc cattattaac cccccagttt cgatttatcg
    27241 cgcaccgcac ctttgtcggc gctggttgcc aggctggcgt aggcgcgcag ggcaaaggaa
    27301 acctgacgtt cacggttttt cggcgtccag gctttgtcac cccgagcttc ctgcgcttca
    27361 cgacgcgccg ccagttcggc atcgcttacc tgtaactgaa tgccacggtt cgggatgtcg
    27421 atagcgatca ggtcaccatc ttcaatcagg ccaatgctgc cgccgcttgc cgcttccggt
    27481 gagacgtggc cgatggaaag accagaggtg ccgccagaga aacgaccgtc ggtgatcagc
    27541 gcacaggctt taccgagacc cattgatttc aggaagctgg ttgggtagag catttcctgc
    27601 atgccagggc cgcctttcgg gccttcatag cggatcacca ccacatcgcc cgcgacaact
    27661 ttgccaccga ggatcgcttc taccgcgtcg tcctggcttt cgtacacttt cgccgggccg
    27721 gtgaatttga ggatgctgtc atcgacgccc gcggttttaa cgatgcagcc gttttctgcg
    27781 aaattaccgt agagcaccgc caggccgccg tctttgctgt aggcgtgttc cagcgagcgg
    27841 atacagccat tggcgcgatc gtcatcgaga gaatcccaac ggcaatcctg cgagaatgcc
    27901 tgtgtggtac gaatgcctgc aggacctgcg cggaacatat tttttaccgc gtcatcctgg
    27961 gtcagcataa cgtcgtattg ttccagcgtt tgcggcaacg tcaggccaag tacgtttttc
    28021 acatcacggt tcagtaaccc cgcgcgatcc agttcgccga gaataccgat aacaccacca
    28081 gcacggtgaa catcttccat atggtatttc tgggtgctcg gcgcaacttt acacagctgt
    28141 ggaaccttgc gggaaagctt atcgatatca ctcatggtga agtcgatttc cgcttcctgc
    28201 gccgccgcca gcaggtgaag tacggtgtta gtcgatccac ccatcgcgat atccagcgtc
    28261 atggcgtttt caaacgccgc tttgctagca atattgcgcg gcagtgcact ttcgtcgttt
    28321 tgctcgtagt aacgtttggt caattcaaca atgcgtttac cggcattgag gaacaactgc
    28381 ttacggtccg cgtgggttgc cagcagcgag ccgttgcccg gctgcgacag gcccagcgct
    28441 tcggtcaggc agttcattga gttagcggta aacatcccgg agcaggagcc gcaggtcgga
    28501 cacgcggaac gttcaacctg atcgctctgg gagtcagaaa ctttcgggtc tgcgccctgg
    28561 atcatcgcat caaccagatc gagtttgatt atctgatcgg acagtttggt tttcccggcc
    28621 tccatcgggc cgccggaaac aaagatcacc ggaatattca ggcgcaggga agccatcagc
    28681 atccccgggg tgattttgtc gcagttagag atgcagacca tggcgtcggc gcagtgggcg
    28741 ttgaccatat actcaacgga atcagcgatc agttcgcgag atggcagtga ataaagcatc
    28801 cccccgtggc ccatggcaat cccatcatcc accgcaatgg tgttgaactc tttggcaacg
    28861 ccgccagccg cttcaatttg ttcggcgacc agtttaccga gatcgcgcag atggacgtga
    28921 cccggtacaa attgggtgaa cgagttcaca accgcgataa tcggcttgcc aaaatcggcg
    28981 tcggtcattc cggtggcgcg ccacagcgca cgagcacccg ccatattacg accatgagtg
    29041 gtggtggcgg aacggtactt aggcatactt tatttactcc cagtgtctgt ctcgtaaatg
    29101 ggacggtgcg tgccgtccca ttttttgtat ttattgatta acttgatcta accagcccca
    29161 tttatcttcg gtttcgccag tgaaaaggcc gaagaaggct tgctgaatgc gtttggtaac
    29221 cgggccacaa cggccttcgc caacctgaat accgtctacg ctgcgcactg gcgtgatttc
    29281 tgccgcagta ccggacataa acacttcatc cgccaggtac agggattcgc gcgacagaac
    29341 ctgctcacgc acttcaattc ccagctcttt cgccagtttg atgatggcat cacgggtaat
    29401 acccggcagc gcggaggagg tgaacggtgg ggtgaacagc acaccatctt tcacttcaaa
    29461 caggttttcg cctgcgcctt cagagatata accgttcaca tccagcgcga taccttcctg
    29521 ataaccgtgg cggcgcgctt cgctacccac cagcagggaa gagagatagt taccaccggc
    29581 ttttgccgcg gttgggatgg tgtttggtgc tgcgcggttc caggaggaaa ccatcgcatc
    29641 gatcccctgc tccagcgctt ctgcgcccag atacgctccc cacgggaaag cggcgataat
    29701 cacatcggtt gagtatcccg ctggcgggtt tacacccatg ccaacatcac caacgaagat
    29761 cagcggacgg atataggcgc tggtgagatt gtttttgcgg atcacgtcac gacaagcttc
    29821 catcagctca tcaatgctct gcgaaaccgg gaagcgatag attttggcgg agtcatgcag
    29881 acgctgcata tgctcacgat ggcggaatac aaccggtcct ttgtgcgagt cgtagcaacg
    29941 gatgccttca aaaaccgaag tgccatagtg cagcgcgtgc gacatcacat gcaccttcgc
    30001 gtcttcccag cgaaccatct ccccattgaa ccaaatgtaa tcagctttct tcgtggtcat
    30061 ttttatattc cttttgcgct caggcgcgga tttgttgtga tgtggttgtg ctctggcaga
    30121 tggcaacgtg tgcgacgtcc accagtttat ttaactgact aaacagtaag tcgaccgacc
    30181 gtgggctggc aacggtcaat tcgatattta tattttgtgc atcgctggcg gcagccatat
    30241 tcattgagca gacgtggaaa ccacgatgac gcaccacgcg taaaacacgt tctaaggttt
    30301 ccggattgaa gcgagccgat acattgacct gatgttgcat catgataatt tctccaacat
    30361 ttctgaatta ctggcgccag gcggcaccag cggccagacg ttctcaagtt cgtcgattga
    30421 gacatgaagc aggtatggcc catcactgtt cagcatggtg tcgagtgccg cttcaacctg
    30481 gtctttacgg gtgatgtgtt ggccagggat gccgaaggcg ctggctaaca tgaggaaatc
    30541 ggggttatca gtaagggtgg tttcgctgta tcgttcctga aaaaacagtt gctgccattg
    30601 tcgaaccatc cctaaccgtt ggttatcgag taagacgatt ttcaacggta actgcttgcg
    30661 ttttacggtg cccagctctt gcacattcat catgaaagag ccgtcaccgg agatacagac
    30721 gacagtatcg ttcggtcgtg cgacttgtgc gccaaccgcc gctggtaaac cgaaacccat
    30781 ggtgcctaag ccgctggagg taatgaaatt ttccgggcga gtgtgtgcga tgtgctgcgc
    30841 ggcccacatc tggtgctgcc ccacatctgt ggtcacgacg caatccgcag gtttacgatc
    30901 cgacagttgt tttaacaaca acggcgcgta gatagcgtca ccgggatggt cgtaacgcca
    30961 ggtatgttca tcacgcagct gcgcgcagta ttgctgccag tcattgatat ttaacggctg
    31021 ctgtaatgct ggtaacagag catttaaatc accttgtaat gccacatgtg cctgacgcag
    31081 cttgttcatt tctgccgggt cgatatccat atggataaca ctggcgtgtg gtgcgaaggt
    31141 gttcagtttg ccggtcaccc ggtcatcaaa acgtgcaccc acggcgatca gcaagtcgca
    31201 ctcctgcacc gcgaagttcg ccgctttggt gccatgcatt cccagcatgc ccaggtagta
    31261 cggataatct gcttcaactg cgcccagccc tttcagcgtg caggtggcag gcatttttgt
    31321 ggtagcgaga aattctcgta aagcaggaac tgcctgcgcc atacccacgc caccaccaac
    31381 gtacagcatc ggtttttgcg cttttgccag catctggcgc gcttgctcaa cttcggcatg
    31441 ggggaaagtc acttcgtttt caacggtggt gaaccacggt tccagatcgc cgctggctaa
    31501 ctggatatct tttgggatat cgaccagaac cggaccagga cgacctgagc tggcaacgtc
    31561 gaatgctgca gccatgatgc gcggcaactc ttccagcgac tgcaccagaa agctgtgctt
    31621 ggtacaggct aacgacaatc ccaggacatc cacttcctga aatgcgtcgg tgccgataaa
    31681 cggtgcggac acttgaccgg tgatggcaac gacagggatg gaatctaaca gtgcgtccgc
    31741 aagcccggtt atcaggttgg ttgcgcccgg accagacgtg gcgatacata cgccagtttt
    31801 accggtagca cgggcataac cgatagccgc cattgccgca ccctgctcat gtcggcacag
    31861 caagtgctcc acgccgccgt catacaatgc atcgtaaacc ggcataattg cgccacccgg
    31921 ataaccgaaa acggtgttca caccctgtgc ccgcaacgca tgtaccaccc actgtgcgcc
    31981 attcatagtt agttccccgt cctgaatctt gagaaacaga attttgtgct gttattcatt
    32041 gtctgctcct cggttatgtt tttaaggtca aaaaaaaccc ccggaccttt cggtgcgggg
    32101 gtcttagttc gttaaggctt gatttctaag cctttcctcg tccaagtgca gccccgcacg
    32161 gtgggataat aatcaccacc acgctaatca cgaccaggct aatcactcgt agaagggctg
    32221 tcattttgtc ttttcttgca tcttgttcga aggaatgcct aaagagttac cataggtttt
    32281 gtaaatagta caagatattt tttggccgat ttttat
//
LOCUS       DAZFQD010000045        32139 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.045, whole genome shotgun sequence.
ACCESSION   DAZFQD010000045 DAZFQD010000000
VERSION     DAZFQD010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32139
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.045"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(67..669)
                     /gene="fimB"
                     /locus_tag="ACHKTW_002160"
     CDS             complement(67..669)
                     /gene="fimB"
                     /locus_tag="ACHKTW_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimB"
                     /protein_id="HFO6463183.1"
                     /translation="MKNKADNKKRNFLTHSEIESLLKAANTGPHAARNYCLTLLCFIH
                     GFRASEICRLRISDIDLKAKCIYIHRLKKGFSTTHPLLNKEVQALKNWLSIRTSYPHA
                     ESEWVFLSRKGNPLSRQQFYHIISTSGGNAGLSLEIHPHMLRHSCGFALANMGIDTRL
                     IQDYLGHRNIRHTVWYTASNAGRFYGIWDRARGRQRHAVL"
     gene            2123..2839
                     /gene="nanC"
                     /locus_tag="ACHKTW_002161"
     CDS             2123..2839
                     /gene="nanC"
                     /locus_tag="ACHKTW_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HFO6463184.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            2859..3965
                     /gene="nanM"
                     /locus_tag="ACHKTW_002162"
     CDS             2859..3965
                     /gene="nanM"
                     /locus_tag="ACHKTW_002162"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HFO6463185.1"
                     /translation="MNKTITALAIMMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPRDQATSAFIDGNLYVFGGIGKNSKGLTQ
                     VFNDVHKYNPKTNSWVKLMSHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSATIDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAALVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNKLAPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            4030..5010
                     /gene="nanS"
                     /locus_tag="ACHKTW_002163"
     CDS             4030..5010
                     /gene="nanS"
                     /locus_tag="ACHKTW_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HFO6463186.1"
                     /translation="MNAIISPDYYYVLTVAGQSNAMAYGEGLPLPDREDAPHPRIKQL
                     ARFAHTHPGGPSCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPSIPDNAGVLIVPCCRGGSAFIAGSEGTYSERHGASHDACRWGSDTPLYQDLVSRT
                     RAALAKNPQNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHSYEAIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSTAARRGVISDRFVEAILQFWREK"
     gene            complement(5018..5122)
                     /locus_tag="ACHKTW_002164"
                     /pseudo
     CDS             complement(5018..5122)
                     /locus_tag="ACHKTW_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001586610.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
     gene            5222..6094
                     /locus_tag="ACHKTW_002165"
     CDS             5222..6094
                     /locus_tag="ACHKTW_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015666 - restriction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorothioated DNA-binding restriction
                     endonuclease"
                     /protein_id="HFO6463187.1"
                     /translation="MASSKSLQQAIANIKIWHKGEQRAPHKPLLLLYVLAGYLNGHPR
                     LFDYGSEIYEPLHSLLERFGPQRSHYRPDMPFWRLQGDGFWQLHNAELCSTAGSSRQP
                     PVKELNEYHVAGGFDEQHYALVTGNKKLINTLAQQILEAHFTESIQEEIADELGFDLQ
                     QIRKQRDPLFRKNVLRAYNYQCAICGFNMRHDDTTVALEAAHIKWKQQGGPCEIPNGL
                     ALCAIHHKAFDKGSIGLDENMRVLVSDAVNGGGIVERLFWDFDGKTIALPQVRKNYPY
                     EVFVEWHRKEVFRG"
     gene            6264..8339
                     /locus_tag="ACHKTW_002166"
     CDS             6264..8339
                     /locus_tag="ACHKTW_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000177022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFO6463188.1"
                     /translation="MTLVDSVEAGRLTISELIDALAKDKNYTASRWYQRYRAFTTLLQ
                     QTSTFAEPATDGLVKQLWYERDNGIASIRQGVPSLAEYQQSLPLLRELTERIRQQPDE
                     ETYQYVGNALQQAKENGLLKRMYWSLRNRVFAAFSPENYTSTVDENAFNKAAEFLNQH
                     FHLGLVLTGNWLQKNYELKQAIHAQSPDTDPYYVNMAIWHLYELLRERDNEQKQEKVA
                     STTSITRSEPIENKIILHSPTNVIFFGPPGTGKTFRLQQKMKEYTSHAVPADRDAWLD
                     SRLESLNWMQVITLVLLDLGKRAKVRQIIEHMWFQRKALLNGRNGNLSNTAWAALQSY
                     TVPESLTVDYKNRREPAVFNKTDNSEWFLVDSQLEQVEDLVELYAELKRGPKSAEAIQ
                     RFAVVTFHQSYGYEEFIEGIRARSDESGNISYPIEPGIFMRLCQRANADPGHRYAIFI
                     DEINRGNISKIFGELISLIEVDKRAGMPNAMSLQLAYSGDHFSVPGNVDIIGAMNTAD
                     RSLALMDTALRRRFDFVEMMPDLSLLSEAKVKGIELESLLEKLNSRIEALYDREHTLG
                     HAFFMPVKNALDAGDEEAAFKQLKIAFQKKIIPLLQEYFFDDWNKIRLVLADNQKQDD
                     NLQFVIEKTDDLDTLFGNNHGLRRHDQQSTAYELKDFDQEIWNIPQAYRSIYQPQQTP
                     LDEQAVNHG"
     gene            8332..9675
                     /locus_tag="ACHKTW_002167"
     CDS             8332..9675
                     /locus_tag="ACHKTW_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001617353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrC family protein"
                     /protein_id="HFO6463189.1"
                     /translation="MGEVISVFEYDLLGSGKAASVGAKPVPPQVFNYLEALSLASNQG
                     SQFLKLTSRSGFKLLQVQNYAGMLSTPHGFQLEILPKVGKNLTAANARQTLLTMLSHL
                     PGFRHIQTQQATLQAQRMPLLEIFINQFLHSVSQLLKQGLRSDYVSKQGNLAFMKGKL
                     MLSAQLRHNAVNRHKFCVDYDDYMPDCAANRLLHSALDKLLSLQLSSDNQRWLYELRF
                     AFDGIPLSRDIERDINNLRLERGMAHYNEPMAWAQLILRGMSPSALQGNTKAISLLFP
                     MEAVFESFVAQTLPDELPPHLKVLPQAAIYSLVKHGVKDCFKLRPDLLIQSNKPVQTE
                     MVMDTKWKLVNSSQQTKSLYGLAQADFYQMFAYGQKYLGGNGEMYLIYPAHDDFSQPI
                     PQHFAFSETLKLWVVPFRIMAKRGERMMWPNDVSCTMSPQLDRMAASVSYSGGMR"
     gene            9672..10058
                     /locus_tag="ACHKTW_002168"
     CDS             9672..10058
                     /locus_tag="ACHKTW_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004222267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6463190.1"
                     /translation="MTHEYQRQSLEQLITQQTATPRSAAVVFNENQTLWQTLGFSQSQ
                     VSLWLASLPQSNHLKGPTTATYQVIPDIAAHLVSLLHQAGGRMPLAQVLKKLPAGITT
                     SEQQIRKLAQKHAQIEIKGPLLVLIN"
     gene            10109..12256
                     /locus_tag="ACHKTW_002169"
     CDS             10109..12256
                     /locus_tag="ACHKTW_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HFO6463191.1"
                     /translation="MAKEAKKQDPQQQLEALFNKLVKQHSEFTQPLVELPEHEFAKAG
                     LIQGLVEGNLTPPVMLIALTEGHWEYAADTAKFDDLAYQLLSDIEGDWPVYAVVDDGL
                     NHRILALFGADGSDGTHGADTLPGLEELRSFDRMERDPTFRWSMRVYTRLMQRFDAFH
                     ENVYRVTKDKVNDKNDIIEEVAKLLFLETFRLHHDEDLTFKDDEGNTLRFRDVFNWQY
                     VESHGDKAVAQIKAAFEEFKSHENYVVISDDGSRNPIFSKETHLRLSVAKNYQDLLEA
                     IQNLGPVKTNDGKIAKEHGTLADVSGDLLGRVFDVFLRANFESKGGLGVYLTPNPVKQ
                     AMLEIAMHDIDDDDEMRSRLANGDFRFCDPTCGSFGFGSVALSQIDKWIDFKLVLADD
                     KKESLKQKLRDCAFTGADAAPRMVMLARVNMALQGAPKAQIFYTDNSLTTNALKPNSF
                     DLICTNPPFGTPKFDKGKNGQNSKANYEANMEQVLGGFRPTKKVVDSYNAWYDHVKMK
                     WEDIGDLELDENGEPKWAGYRTDLRRTGGTDKKPFYSLQPTTAGLALGSKPDSKGNWQ
                     PVGATIDPAVLFIDRCLQLLKPGGRLLIVLPDGILCNSGDRYVREYIMGKKDEKTGEF
                     VGGKAIVKAVISLPSDCFKLSGTGAKTSILYLQKRHANPNQPEQFLPEPQTDVFMAVA
                     ETLGYVVKNNIEDYNAGVANDLDKIVSAYKRGE"
     gene            12256..13782
                     /locus_tag="ACHKTW_002170"
     CDS             12256..13782
                     /locus_tag="ACHKTW_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFO6463192.1"
                     /translation="MDKFFRTNSSILVERLDPNFYQPSLVENDKLIKSKGWVELQSLY
                     EASGIGNTSAVERYYSEAADSIPYISGKVIKSFNIDLDECQRISLDSHKNELTKSALK
                     PTDVLVIRKGDMGNACVVPSEVNEANCSSEVIYLKMKASSDPYYLVSYLNCDQGQKAF
                     KRLGRGTIIPGVSLLDVPRLPIPKVSEFVQKYIGDKVRQAEQLRAWAKLLRTSVDAHL
                     NSLNLPINEPPALLNRVSAQTMEDRLDPRPYRTHYLCLVREIEKLPHDSISTLVELAS
                     GCPVSSNDFLENSGIPLVRIRNIGFDDFIGLDTGVSQDVYQDATKYQAKDKMIVVGMD
                     GIFRSQFFISDELPMLVNQRVAMLSPQNIRGELLTHWLNRPEGQMQLNQWAVKTTVEH
                     TSLSDIGRVLIPRLDKSLENKLADYLLNARLAYRYAKFLTQVAKTLVEALIEGQLTEQ
                     QLIQAQQALEDGDNSFDQAILSKLSAEGYAIEGATPLFSDVDELYSLLEEAAQAEAEE
                     "
     gene            13800..15086
                     /locus_tag="ACHKTW_002171"
     CDS             13800..15086
                     /locus_tag="ACHKTW_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6463193.1"
                     /translation="MDSITTRQNNDPVNSEAALNLCLQLWHQGGLTASKAALLLAAVP
                     ALRSLLQPIIQPKKKDAETDIISAFSLTAPLLDAFSDLSQSGEWQLALLGLNPDVRQH
                     WINLAAARCQEAGAMSDPMVLVKLIQQLGNASEWVLAQLENADFSPQIIASPLAQTER
                     DLLGHSLNDNAAIPALCRILRTSHTLFTVSEQNEPPASIQAVDVTAKQLTNNWCSGRL
                     LALPNTLLDEHNLKPNADWLLVSRSGHDNVPLTELFAQQPWLFLLSLIIFVQDAWAAE
                     QRGGLLLTLPAGQNAFAPGQINVAVQGIEGDEVSLGSLAEFLVLLLGELNIPLYPALD
                     ANTESINRLNRVLSSFIAELLAKKIWQFTEAGRGESGQYRIHTSFSDACYSLPLAPLF
                     GYKSQTLQRAIKQLAQNCYANKKRAANRINLQGSSL"
     gene            15083..19369
                     /locus_tag="ACHKTW_002172"
     CDS             15083..19369
                     /locus_tag="ACHKTW_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6463194.1"
                     /translation="MSFELNEQMVDSLAKQHRWAATGVSDYPPRDPLVGQSRFFKRFQ
                     TFLHTVDHDDDRFAHVFAVEAEWGRGKSRLGHELVAQINDCSRGWFVRDEQGQLHDKQ
                     LFNRDTQDKYLALYIRYSQVASDYQNSDNWFAFGLYQALLPLATQTFDGSIQSEIAKQ
                     AYERLMPFGFKSELLADALQLNANHSEEDLYTDQTLVVSLVQAAYKVLKSFGVQYMLV
                     VLDELETVAEAATFGLEDEQNKRLDGQAIRLIGKAIKEEDPRRKLPWLRYVALCSPLL
                     GQQLREIQSVARRFELVELEHNAFADVSDYVASLLKDGKLGFNYPAGLVEAAYAMSGA
                     NFGWFNVVMANVDAVLSQYQQAGRSIKDVGDLFEAVLAGSGRVARHVLDKGAIEGIQT
                     RDQALLTECRSLLYGQLPVRLAEMAYATELLALKNEDLEPIAAHYRKLSIDRLQCRQA
                     LEQAKFRRDKDEWYYPAVDQALNLDTLLDNLRTFSIKEQAASGDKGAVLLPLSKGEFK
                     YLLSLLYDHPAIEFAADALWHKLVGETTELDASEATHIGPSVAMLLRLDLRYRSAQQN
                     SLIFKDPAMGDAHEQAMSELSKQSSQKPLIKALARVTGFVRLLDNNWTYDENLLSNKA
                     GSDDAPLAILTEPRGRQGGLLTLDGFKLHPEGKALFAWVSNLIELNNLHTFAANHSHQ
                     NGRTPVLALTASAHLMEQYSRLDERNELRDAILLHYVNPSEADQLERIGLSLAACQLH
                     GVNLTADSFTAKFKNKLHALTTFATDAIHKWRQRLQQRGLIAWPLKVDGKLSPNDRDL
                     LFKGWYQLAIAHPELNGILDLQQQHGVPVNELSSLLDKLKVPGSYIAKGYTADEHAGL
                     FTELNNVQRSQAQIPLFLARIAHPNKKHKWQFEGFKQQFYFAYVAETSVTAKGVFNDW
                     MWWCGELNLLTLTNPTEKQAIWEHYPRSRLENAIREAQNWFRGNDMGSYAANVEVMSR
                     VYGYARINEMFAPLGKNKLGFVTVEAKEQLEKAQSLFNVLKQQEEQLADMVEANDTKV
                     LAGLIHKRAEVLELVAKVKPLNSSRPMLKDAHILSLEDKTTSLYQRIEQACLFAEFVE
                     CSAERINNRLVDLIIDVETECAPLTNFPKRLYTNTLRTIGHILDGALKDDTSSATGRK
                     EQQADSDTLLHYLRKLDLGRAHDKLSALAQEVGLNLHNDQQLPIAEIQGHILSSYRNC
                     KERFSKLVNNLTEQKLRAQQLQEWLSSATAEYQFADDIAELPKLVMKLQLIEDAIADL
                     PSDVESKRQSMQNSLRNGQFSSLRDLPEELLKPARGQLTPIQGQLLKIEERLNQVRRN
                     SIAQVNSWLPLLKPLLASQKQAEPAALTLEDVSNLGVRELQQVCESTQAKWQSQGEQI
                     LNDTGLTLADWQPIYKALSQNQEPVLTPEQQKGLVDKGIVKMRLTFATGL"
     gene            19380..20228
                     /locus_tag="ACHKTW_002173"
     CDS             19380..20228
                     /locus_tag="ACHKTW_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004222254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6463195.1"
                     /translation="MSRYLSAALASNRKGRFLQTVAGATPLMKDWISSLPASGLLIVQ
                     AEELTDANTMQHLYHWAMQAGCAALVINLKAEQFTLLAQLPYPLDWQLVPASLRGQEP
                     GLTALLASETDQAIAGFTGSADRYQHQAGDVVHTRYIRKHSNSGLLAFTTLPLWSLTL
                     LDHSELLVSWLNWFVDHAGIAERIIEPKAPSTDYTPDKHDLVVLLLLYAGGGMNLQAL
                     SEHNAVKLMFDVNSLDIVKRGEMLRQHDFIDDAGITATGKTCLQASQYWAYAPLLGEQ
                     LHTGTL"
     gene            20231..21388
                     /locus_tag="ACHKTW_002174"
     CDS             20231..21388
                     /locus_tag="ACHKTW_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001004905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6463196.1"
                     /translation="MNENKTMNLAQSLAIDSQLRLPGSAGRLLKQVRDIEQVAPLFRD
                     AGLVKAVPKPTIVNLRVAGIATKSCLDKVLGGFIYASAAVRTDLLIDDNKVSTVGSDS
                     VSELDDIDFEAQRKRLDLIEIRQAYQLVEKKLLGYEPGDLILLDTPLFLARDMAPLER
                     NVRHKQEYEKTRQVIETFWQNHRSRIFPWNQNGVVLASILAERFSAIVSIAKQDLRTE
                     QGRKQILASDGFSKELMQNLEGLDESLVGVGDLRFINGILGNYSRTIAFRLSEQEKRI
                     EPNIEAKQGLIGFHFRSARGGQIKMVQLAGDEPEWKSADLDLVASRLMVLDMQNKGNA
                     MPLPQLLGYQQLGILPKFATFYRQGLHQALKNNDIEAGWLAGLDEGLNNGL"
     gene            21378..23063
                     /locus_tag="ACHKTW_002175"
     CDS             21378..23063
                     /locus_tag="ACHKTW_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019106735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HFO6463197.1"
                     /translation="MAYKVLGKLVGNTGDASQLTMVVQDSFSVRRGEFVRIMHQERKD
                     EGQVAVLGRVTKISRTNMLYNAGFGDGVTELELLPGANVTGENMFAQVELVGYRDPVT
                     RQIKIPRRPLNPGTAVETVDFQFLSDFYEFNEHSSLHLGNLVGYDKGENTVPVFIDVN
                     KLVTEHLAVLAMTGSGKSYTVGRIIERLVAVNNGTVVVFDPHGEYGKALAGGNLQFSS
                     FLEATDDKRDQEALPLIKQTFEKLQAAGAGIQVYSPQHESFKHKYASKNTPLALQFDH
                     FEMDDIAEILPGLTEPQQRVLDVAIRYWRYADKTEPRDINRLRFLLGDGIEELKEWDD
                     LSEAEAKALSGRSAAVASMKLSRVLNEAKSFYSATMAEPTDIYKMVGRPSNQQGRLVV
                     VDLQGLSDTAKQVICALLSSEILKAASSKTDPLRPCFIIYEEGHNFAPAGGNAVSHRI
                     IKKIAGEGRKFGVGFGIVSQRPSKLDSDVTSQCNTLITMRLKNPDDQRFIAKASDMVS
                     KADLEELPSLSTGEALVCGRSIPAPLLVKVGSKALIHGGESPEVLRVWGSFSG"
     gene            23071..23613
                     /locus_tag="ACHKTW_002176"
     CDS             23071..23613
                     /locus_tag="ACHKTW_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001115385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6463198.1"
                     /translation="MPEVAWVNETFPLLASGKLLDQDVLVHSLGCSVWNTFGHEQSFM
                     AVVECPAPGTFGADIRSDSGWFHKSSASPVCLIEFERFDGSAKGQQKLEEKLKNLLEA
                     AQRWNHCPKTLVLSAWSQGLVGVPDTQKLKDICRMGFTSSTGTQVIAAPDVEVVFSRF
                     LFIKNLNMIVLDRIHYEVLM"
     gene            23610..25079
                     /locus_tag="ACHKTW_002177"
     CDS             23610..25079
                     /locus_tag="ACHKTW_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001022617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HFO6463199.1"
                     /translation="MNIKSAHFLPGTNKRLGDKYLLLECLGDGSHGWVWRAERLKDGK
                     IVAVKIPKDITREDRQLAEGKELLNVEPHENIVQIFDMGRIDNEWFYIEMEYFPSQTL
                     AQKLDDRQRNFGQTYERLFRIYRQVLCAVHYLAELPVPISHGDIKPHNILVGERDLVK
                     LTDFGSSALPEEIYVRTRENGGTVLYSAPEFSNVDSRRGSLEELLLGDIYSLGVLLYQ
                     LLTGKLPHDTPAQVQRHAPFKLPTEINSSIHTDLEQVVLTCLQKRAEDRFSTISDLIH
                     AFDSAATKQLKVGAIAPVFQTKPAQDWSSAVLEAMSDQSYQKAAQLASQEYSRSKDLQ
                     ALHQQLIALYRANRLFDFEKVVEDNKAILLEGKLTQPAALFELIVKANLQLRNISQAN
                     FWLLQRKQAEAENAATMYLESSILGLEAKYSEARVLLEKVNQTTPMKFHVLSQLILVC
                     EQMRDYSGAAAYLKAALRVAPLDNSMKEKKELYAKLGVI"
     gene            complement(25280..26545)
                     /locus_tag="ACHKTW_002178"
     CDS             complement(25280..26545)
                     /locus_tag="ACHKTW_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003924703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO6463200.1"
                     /translation="MALSDVKVRSAKPEAKAYKLTDGEGMVLLVHPNGSKYWRLRYRF
                     GGKEKMLALGKYPEVSLVDARGRRDEARKLLANGVDPSENKKAVKVEHEQEVITFEVV
                     AREWHASNQKWSASHSARVLKSLEDNLFAAIGKRNIADLKTRDLLVPIKAVESSGRLE
                     VAARLQQHTTAIIRFAVQSGLIDYNPAQEIAGAVATAKRQHRAALELNRIPELLQRIY
                     HYSGRPLTRLAVELTLLVFIRSSELRFARWSEVDFDTAMWTIPGEREPLEGVKHSQRG
                     SKMRTPHLVPLSRQALTILEKIKSMSGNRELIFVGDHDPRKPMSENTLNKALRVMGYD
                     TKTEVCGHGFRTMACSSLIESGLWSRDAVERQMSHQERSSVRAAYIHKAEHLGERMLM
                     LQWWADYLDANRMAAITPFDYAKINSSFR"
     gene            26907..28178
                     /locus_tag="ACHKTW_002179"
     CDS             26907..28178
                     /locus_tag="ACHKTW_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001735688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFO6463201.1"
                     /translation="MSFKRDRYLISDSCGNYLVRIAIPKYMRHLFGGRCSYVKSTGTR
                     DIREARLFRDAVALEWIKLRNKLKPQREKTSKIEQIITELRKAGTYAFQVSESFNDSS
                     IGCPSLLNMRDQYILQFSEKRKLTTLSKTNKAVEVLLEHLNKRDVQLRDVNRTIVTSW
                     LDNLKNEKAPQTIQNYISALAQIWDLARNRYHDAPQDNPWRGHGLEAKSSKVNYEAFS
                     NDELAKVFSMLADEEEMQNITLIGMYTGMRINEIASLTIDDVKEIEGVLCFEITQGKT
                     KAAARVVPVHSLITPLVLSLREKPHNGFLFYHASITERADGKRSTWHTQRFTRAKRKA
                     LGEKGTERKVFHSLRHGVAQLLDRNQIPEDRIALLLGHTRGNTETFRTYSKNAASPVE
                     LKNYIELLRYPEIEKGLSINKKSNLRRKTAP"
     gene            complement(28258..28971)
                     /locus_tag="ACHKTW_002180"
     CDS             complement(28258..28971)
                     /locus_tag="ACHKTW_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate protein"
                     /protein_id="HFO6463202.1"
                     /translation="MATAGILTIRAANTPEQHVQAVYKAEQDINSTKNENSKANKTKG
                     ENGFAIVTSGLASSGNDVYENYMALAFDSVDDVNVRRSADVTSYPVENGATVSDHVQI
                     KNNKFSLKGRITETPIKSDPGLLKSAGVNGNRRSLAIDYLNQIMDSRQPFLLVTENKT
                     FENVVLTGIEYTEEASESLVFDLSFEQIRLVSYGTVNTVAIKTQPSKNIGANMKKRVN
                     TEKSSSEGEDTITPAFKQE"
     gene            complement(29003..30838)
                     /locus_tag="ACHKTW_002181"
     CDS             complement(29003..30838)
                     /locus_tag="ACHKTW_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000890067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6463203.1"
                     /translation="MLEINTSKIKNTVTFSVDKDSLKKAKDSITGLKEFAENIKPAKL
                     RFDNVTKGYKKAQSEVDKIAQQKAKNEKQNQRAQAAAARAQARQQQQIAARKEKAELK
                     LLDVSSSISAMHRLSVAEQYKAIAQAREIAKQYEQGAISLARMNSQMKRLQQQQRKIN
                     GNRKAQLAPVKGGGSGNSATMGALLFGGATAAAGVMAVSKASDFVTSSFANAETQGEL
                     IQRAKLGGVDVNQMYNITEWAYRHGIDSLQGDQGARKYLDQMKDVRERAAKSYSEAEL
                     VKDKQTGKSKWKGGDNAINELLNIGVINKSDLKKFADNPAGLISKAVNGMMKKGFSDS
                     QIGQRLEDLGDDLMLTSKYWQRSVKDVQESINQQKASGKWLTETQQESIVKFRELNRQ
                     LSQLSDARQVAFVDGFMKSLDPKVTEEFMKNLSNLTPYFTKLGEASGSLFEAIMKIVN
                     WFNRNDDKTDAIQKNLGDAPPLSNEGMKQHLSNLVPDQYKGAGTATTAPDNSHSLFNT
                     IKGWLFDDKSSSVSDVKMSVSEAPITNLKQGALNNLAMTTPAYNLSPVFNLNPTFEVV
                     TEVPLTINSDTSRLSDYVDFTARASRDSFLKSLTLTSLSGQSNGG"
     gene            complement(30858..31055)
                     /locus_tag="ACHKTW_002182"
     CDS             complement(30858..31055)
                     /locus_tag="ACHKTW_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001350140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6463204.1"
                     /translation="MEVILKSKNGVHVHLDANDSRGLLNLKYLCSLLDVPYEGVKARM
                     FRLNESIDQALHHFLSKEGGK"
     gene            complement(31143..>32139)
                     /locus_tag="ACHKTW_002183"
     CDS             complement(31143..>32139)
                     /locus_tag="ACHKTW_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFO6463205.1"
                     /translation="PLFSAHSTRNYLLSTLDFTDPVGVSSHKVAVSQLVENNESLFNK
                     ETSRFSSEHNVTKREQGKEWLIEIPYFLREDVIRPSDVQGKRKPGTDFQETLTDIYAE
                     YVAKHHVAYQRTKESVLAASLFSGRTYTPKTDDVLIEWGKLFNVSAMKATVNASSTDT
                     TKIFKEFDQIATDIIEKAQSQAAAVERIVVFCKPEAFSAIRFSAGMANAFQYVSPLEE
                     GNVVYQRRDLLPGVTAFTIPGTNIDVIKLVDPLHLAHMTADAVAVPKFAKGAGVYANI
                     FGAASSTFELINAAPAEVYSYSYESSRGDAVNVVTENSQMVVNHGVGFSVQITVK"
BASE COUNT         9135 a         7142 c         7329 g         8533 t
ORIGIN      
        1 aatcattttt gatatatcgt aagaataatg tagtttttaa caccatccct ggtatctcaa
       61 ctatctctat aaaacagcgt gacgctgtcg tcctctggct ctatcccaga tgccgtaaaa
      121 acgccctgca ttgctggcgg tataccagac agtatgacga atattgcgat gcccaagata
      181 atcctggata agtcgcgtat ctattcccat attcgccaaa gcaaaaccac acgaatggcg
      241 taacatgtgc ggatgaatct ccagtgacaa cccggcatta ccaccggaag tcgagataat
      301 atggtaaaac tgttgccgag aaagcggatt tcccttacga gataaaaata cccactcgct
      361 ctcagcatgc gggtacgaag tacggatact caaccagttt tttaaagcct gaacttcttt
      421 gttcaatagc ggatgcgttg ttgaaaagcc tttttttaat cgatggatat atatacactt
      481 tgccttaaga tcaatatccg aaatcctcaa tcgacaaatt tcactcgccc ggaaaccatg
      541 aataaaacaa agcaaagtca gacaataatt acgtgctgca tgaggcccgg tatttgctgc
      601 tttaaggagt gattcgattt cactatgggt caggaagttc ctttttttgt tatcagcctt
      661 attcttcatc gtttttccct tataattaca gacgcgcact agctgtgctg ggttagcaat
      721 aatccaacat ttttatcctg attatgttta taaaagcgaa cgtttgctta ataactaacg
      781 taagtggacc agttcttctg agtgaactta aatggagtag caacagttaa ttataataac
      841 gttgccatac aaacgccatg ctcttgcatg ctatgtacct ttatatattt atcaatcgga
      901 tacgaatcta ataataaccc tcatcattaa tgcaactaat cttatctata tatcatgtga
      961 tatgttctgt aacaagtaaa atcaacataa aaaattcagc aaaatttaat attcgttcaa
     1021 ctagccgtct cacatatgac attattagtc aactccattt gccacaaaat ggctatttca
     1081 tggagcgcag taatttctta gtggctatta tcatgctaat tcttaggtcc ctagcgatta
     1141 ttcctggctg attcgtcagc ttacgccatg agattgttac attccttttt atcctggaga
     1201 tatggggaac gcaggagatg attttttcat ctacattcgt tggtaaatat agctttggtc
     1261 actacgactt aaaccttcct caatcacagc cattcgccgg gcgatacggc aaacaaacta
     1321 agtgaaacat catcctgaaa acaccaacat caacaagcct ctccagatcg acttcagaag
     1381 tgaccagtta caagccacaa accaggaaac atatttcatg atgagaatta tgctcagcct
     1441 gatggcgggt aaacactgat tttcaccacg ctcactgagt tagcttgcaa atctttcctt
     1501 gtttatactt taaataaatt acttataaaa cagataatta aacaacaatc tttggtttgt
     1561 gaaggcctcc gcaagtgttc gtctggcttc acatggcatc ttcttcttag aaaaagatcg
     1621 acatattttg tgacacgaat tgcaaatctg gtttcgttgt atggattgcg tgatttttga
     1681 tctggtataa catgtataaa ggtacaccac gataggcaat gagactgggc atctcgtaat
     1741 ctatggcaaa catcacttca gttctttctc atcgggtgat gaaaatgcac ttcagtctga
     1801 aaggaatatg aaaatgagat caacagacac tctattttat gactctgggt aaaatgaatt
     1861 gagtaagtga tatagcttac gaacattcaa atcaattaaa catcagcaga gattttatac
     1921 tcaagtattt aatctggctc tctgtttatt taaataatgt ggaaagagat ttttcacagg
     1981 agacattata caaaaaatat aaaatacagc tatcggttgc caaagacact ataagcctgg
     2041 caaaaaatac tacacaacat aaatgctaat tgtttatgcg ggctttgtat tgctttctgt
     2101 atcctacaaa tgagtgaaat ttatgaaaaa ggctaaaata ctttctggcg tattattact
     2161 gtgcttttcg tcaccattaa tttctcaggc tgcaacactg gacgtacgtg gtggatatcg
     2221 tagtggaagc cacgcctatg agactcgact caaagtcagt gagggatggc aaaatggatg
     2281 gtgggcaagc atggaaagta atacctggaa taccattcat gataataaaa aggaaaatgc
     2341 cgcactcaat gatgttcagg ttgaagttaa ttacgcgatt aaacttgatg atcaatggac
     2401 ggtgcgcccg ggaatgttaa cgcattttag cagcaacggc acacgctacg gaccctacgt
     2461 aaaactgtcc tgggacgcga caaaagatct taattttggc attcgctatc gttacgactg
     2521 gaaagcttac cgacaacaag acttatccgg tgatatgtct cgtgataacg ttcatcgttg
     2581 ggatggatat gtcacttacc atattaatag tgatttcacc ttcgcatggc aaacgacgct
     2641 atacagcaaa cagaacgatt atcgctatgc aaaccataag aaatgggcga cggaaaatgc
     2701 atttgttcta caataccata tgacgcccga tattacgcca tacatagaat atgactacct
     2761 ggaccgtcag ggtgtttaca acggcagaga taatttatcg gaaaacagtt atcgcattgg
     2821 tgtgtcattt aaactgtagt agacaggaga cagtcacaat gaataaaaca ataacggcgc
     2881 ttgctatcat gatggcttca tttgccgcaa acgcgtctgt attacctgaa actcctgtgc
     2941 catttaaaag tggtaccgga gcaattgata acgacactgt ctacattggt ttaggtagcg
     3001 caggtacggc atggtacaag ctggatacac aggccaaaga taaaaaatgg acagcgttag
     3061 ctgcattccc tggtggacca agagatcaag caacctcggc atttattgat ggcaatctgt
     3121 atgtgtttgg cggcattggc aaaaatagca agggcttgac tcaggtattt aatgacgtac
     3181 acaaatacaa ccccaaaacc aatagttggg ttaaattgat gtcgcatgca ccgatgggca
     3241 tggcgggtca tgtgactttt gtacacaacg gcaaggctta tgttactggc ggtgttaacc
     3301 agaatatctt caatggctat tttgaagatc tcaacgaggc tggaaaagat tcagcaacta
     3361 tagacaagat caatgcccat tattttgaca aaaaagcaga agattatttc ttcaataagt
     3421 ttctgttgtc ttttgatccc tcaacacagc aatggagtta cgctggcgaa tctccctggt
     3481 acggaacggc tggtgcggcg cttgtgaata aaggtgataa aacctggctt attaatggcg
     3541 aagccaaacc aggattgcga acggatgccg tatttgaact tgatttcacc ggtaataatt
     3601 taaaatggaa taagcttgct cccgtctcat caccagatgg cgtcgctggc ggttttgcgg
     3661 ggataagcaa tgattctctt atatttgccg gaggggccgg attcaaaggt tcacgagaaa
     3721 attaccagaa cggtaagaac tatgcgcatg aaggcctaaa aaaatcatat agcactgata
     3781 ttcatctttg gcataacggg aaatgggata aatcgggtga attatcgcaa ggtcgggcct
     3841 acggagtatc attgccctgg aataatagtc tattgattat tggcggtgaa actgcaggcg
     3901 gcaaagcggt gacggattca gttttgatct ctgtgaagga taataaagtc acagtacaaa
     3961 attaacgctt cagggccccg gtaaaggggc cctgctatca atttgtcatt tcaattacgc
     4021 gaaatttata tgaacgcaat aatatcgccc gattattact atgttcttac cgttgctggt
     4081 cagtctaatg ccatggcgta tggcgaagga ctgccattac cggacaggga agatgcgcct
     4141 catcccagaa ttaaacaatt agcgagattt gcgcatacgc atcccggagg cccgtcatgt
     4201 cactttaacg acattattcc actgactcac tgcccacacg atgttcagga tatgcagggt
     4261 tatcaccatc ctctggcaac gaatcatcaa acacagtacg gcaccgttgg ccaggcactg
     4321 catattgcac ggaaattact gccctctatt cctgataatg caggggttct catcgttcca
     4381 tgttgtcgtg gcggctcggc ttttatcgcg ggcagcgaag ggacatattc agaacggcac
     4441 ggagccagcc atgatgcttg tcgttgggga tcggatactc cgctatacca ggatttagtc
     4501 agcagaacgc gagccgcact ggcaaaaaat ccgcagaaca aattcctcgg cgtatgctgg
     4561 atgcaaggcg aatttgactt aatgaccagt gactacgcgt cacaccctca acactttaat
     4621 catatggttg aagcctttcg tagggatcta aaacaatacc attctcagct taataatatt
     4681 actgacgcgc cgtggttttg cggcgatacc acctggtact ggaaagaaaa tttcccgcat
     4741 tcgtatgaag ctatttatgg caattatcaa aataatgttt tagccaatat tattttcgtc
     4801 gacttccagc aacaaggtga aagaggactg acgaacgcgc ctgatgaaga cccggacgat
     4861 ttaagcacgg gatattacgg ttcagcgtac cggtcaccgg agaactggac gacggcactg
     4921 cgaagcagtc atttcagcac ggcagcccgt cggggggtta tttctgacag gtttgtagaa
     4981 gcaattttgc agttttggcg cgaaaagtga gcgttcatta atttgattta ttcgcatcca
     5041 tcaacgcttt taactcctcc ggcgtaaaag gtggtttgcg tcggtgcaac atcgcatggc
     5101 aattagggta cacaggcagc aaaaaaacaa atgagatcaa agtaatatat cttcacttgc
     5161 aggcttgaac agccgaaaat ttgcgagcat gatcgcaatt atttcactgc cagggatatc
     5221 aatggcttcc agtaaatcgc tacagcaggc aattgccaac ataaaaatct ggcacaaagg
     5281 tgaacagcgc gcgccgcata agccattgtt attgctatac gtattagcgg gatacctgaa
     5341 tggacatcca cgccttttcg attatggatc ggaaatctac gaacctctgc acagtttact
     5401 ggaacgtttt gggccacagc gttcacatta ccgacctgat atgccgttct ggcgattaca
     5461 aggcgacgga ttctggcaat tacacaatgc cgagctttgc tcaacagcag ggagcagccg
     5521 acaaccgcca gtgaaggaac tgaacgaata ccacgttgca ggcggtttcg atgaacagca
     5581 ctacgccctg gtaaccggca ataaaaaact catcaatact ctggcgcaac agatactcga
     5641 agcgcacttc accgagagta ttcaggaaga aattgctgac gagctgggtt ttgatctcca
     5701 gcaaatccgt aaacagcgcg accctttatt tcgcaaaaat gtattacgcg cttataacta
     5761 ccagtgcgcg atatgtggct ttaatatgcg ccacgacgac accaccgtag cgttggaagc
     5821 ggcgcatata aaatggaaac agcaaggagg tccttgtgaa attcctaatg gcctggcgct
     5881 ttgcgcaatt caccataaag cctttgataa aggatcgatt ggcctggatg aaaatatgcg
     5941 ggtactggta tcagatgcgg taaacggcgg agggatcgtt gagagattat tctgggattt
     6001 tgacgggaaa actattgcgt tgccgcaggt gcgtaaaaat tacccttatg aggtgtttgt
     6061 agagtggcat cggaaagagg tttttagggg gtgaggttga taacagcatt caccctacaa
     6121 ccttacattg tatttaaatt agagctgagt taacagatag ttgtatatat aacaggcact
     6181 tgtcacaacg attaactcaa tatagttaga atacctcata ttaattaaaa aataagtaat
     6241 gtcacaacag aggaaaagtc acgatgactc ttgtagatag tgtcgaagca ggtaggctaa
     6301 cgattagtga attgattgat gccctggcga aagataaaaa ttacacagct tccagatggt
     6361 atcagcgata ccgtgcattt acgactttgc tacagcaaac ctcaactttt gctgagcctg
     6421 caacagatgg tctggtcaaa cagctttggt atgagcgtga caacggcatt gcaagtattc
     6481 gccagggcgt tccatcctta gcagaatatc agcaaagcct gccactgctt agagaactaa
     6541 ctgaacgaat tcggcaacag ccggatgaag aaacttacca atatgttggc aatgcacttc
     6601 aacaagctaa agaaaacgga cttctcaagc gtatgtattg gagtttgaga aatcgcgtct
     6661 ttgccgcgtt ctcgccagaa aactacacca gtactgtgga tgagaatgct tttaataaag
     6721 cagcagaatt cttaaatcag cacttccatc tcggtttggt actgaccgga aattggttac
     6781 agaaaaacta tgaattgaaa caagccatac acgcccaatc tcctgataca gatccttatt
     6841 atgtgaatat ggccatctgg catctctatg aattgctccg tgaacgcgat aatgaacaaa
     6901 agcaggagaa agtagctagc actacatcca taacccgcag tgagcccatc gagaacaaga
     6961 tcatcctaca ttcaccaact aacgtgatct tctttggccc ccctggcact ggcaagacct
     7021 tcaggttgca gcaaaaaatg aaagagtaca cttctcatgc tgttcccgct gatcgtgatg
     7081 cctggctgga ttctcgcctt gaatcgttga actggatgca ggttataacg ctggtgctgc
     7141 tcgatcttgg gaaacgagcg aaagttcgcc aaattattga acatatgtgg tttcaacgta
     7201 aggcattatt aaacggtcgt aatggcaatc tatcgaatac tgcctgggca gctttgcaat
     7261 cctatacagt tcccgagtcg ttaaccgttg attataagaa tcggcgtgag cctgccgtat
     7321 ttaacaaaac agataacagc gaatggtttc tagttgattc acagctcgag caagtggagg
     7381 atttggtaga gctctacgcc gaacttaaac gtggccctaa atctgccgaa gccatccagc
     7441 gttttgcggt ggttacgttc caccaatctt acggctatga agaatttatt gaaggtatac
     7501 gcgctcgctc tgacgagagt ggcaatatct cttatcccat tgagccgggt atctttatgc
     7561 gcctttgcca acgtgcgaat gccgatccag gacatcgcta cgccattttc attgatgaga
     7621 tcaatcgcgg taacatatcc aagatctttg gtgaactaat ctcactcatt gaagtagaca
     7681 agcgtgcagg catgcccaat gcgatgagcc tgcaactggc ttatagcggt gatcacttca
     7741 gcgtacccgg caatgtcgat atcatcggag ccatgaatac agcggaccgt tctttagctc
     7801 tgatggacac ggctttgcgc cgtcgctttg actttgtcga aatgatgcct gatctctctt
     7861 tactgagtga agctaaggtg aaaggcatag agctcgagtc gttgttagag aaactcaata
     7921 gccgcatcga ggctctttac gatcgtgaac atacgctggg gcatgcgttc tttatgccgg
     7981 taaaaaatgc actcgatgcc ggtgatgaag aagctgcgtt taaacaattg aagatcgcat
     8041 tccagaaaaa gatcattccg cttttacagg aatacttttt cgatgactgg aacaagatcc
     8101 ggttggtgct ggcagacaat caaaagcaag acgacaacct gcaattcgtg attgagaaaa
     8161 ccgacgatct cgatacgctt tttggtaaca accatggttt acgacgccat gatcagcaat
     8221 caacagctta tgagctcaaa gatttcgatc aagagatctg gaatattcca caggcttatc
     8281 gttcaattta tcagccccaa cagactcccc ttgatgagca ggcagtaaat catgggtgaa
     8341 gttatctccg tttttgaata tgacctcttg gggagtggaa aagcggcttc agttggcgca
     8401 aaaccggtgc ctccacaggt ctttaattat ttagaagcgc ttagcctggc aagtaatcaa
     8461 gggagccagt tcctcaaact cacctctcgc tcaggcttca aattgcttca ggttcaaaac
     8521 tacgcaggga tgctttcaac acctcacggt ttccagcttg agatactgcc caaggttggt
     8581 aaaaacctca cagcggctaa tgcacgtcaa acattgctga cgatgctaag ccatctgcct
     8641 gggttccgac atattcaaac ccagcaagcc actcttcagg ctcagcgtat gcctttgctt
     8701 gagattttta ttaaccagtt tttgcatagc gtcagccaat tgcttaaaca gggtttacgc
     8761 tccgactatg tgagcaagca aggtaatctg gcttttatga agggcaagct gatgctttct
     8821 gcacagctgc gacataacgc ggtgaatcgc cataagtttt gtgtcgatta tgatgactac
     8881 atgcctgatt gtgcagccaa tcggctctta cactccgcac tagataagct tcttagtctg
     8941 cagctgtcat cagataatca acgctggctt tacgaactgc gttttgcgtt tgacggtatt
     9001 ccacttagcc gggatattga acgtgatata aacaacttac gcctcgagcg tggtatggct
     9061 cattacaacg agccaatggc atgggcgcaa ttgatcctga ggggaatgag cccgagtgct
     9121 ttgcagggaa acaccaaagc gatatcactt ttgttcccta tggaagcggt atttgaatcc
     9181 tttgtggcac agaccctacc cgacgagtta ccacctcacc tcaaagtttt acctcaagca
     9241 gcaatttatt ctttggtaaa gcatggagtt aaagattgct ttaagcttcg ccccgacttg
     9301 ctgattcaat caaataaacc agttcaaacc gaaatggtga tggatacaaa atggaagctg
     9361 gtgaatagca gccagcagac aaaatcactc tatggactgg cacaagctga cttctatcaa
     9421 atgtttgcct acggacaaaa atatcttggt gggaatggcg aaatgtatct gatttaccct
     9481 gcgcatgatg actttagcca accgataccg cagcattttg ctttctcgga gactttaaaa
     9541 ttatgggttg tgccattccg gataatggca aaacgcggtg agaggatgat gtggccgaat
     9601 gacgtgtcct gtacaatgag cccccaactg gatcggatgg ctgcttcagt atcctatagt
     9661 ggaggcatga gatgacccat gaataccagc gacaaagcct ggaacaacta ataactcagc
     9721 aaacagcaac tcccagaagc gccgccgttg tatttaatga gaaccaaacc ctctggcaaa
     9781 cacttggctt tagccagtct caagtttcgc tatggcttgc ctcattgcct caaagtaatc
     9841 atctaaaagg gcccacaacg gcaacgtacc aggttatacc tgatatcgcg gcacatttgg
     9901 tatcattgtt gcatcaagct ggtgggcgca tgccccttgc acaagtattg aaaaagctcc
     9961 ctgctggtat tacgaccagt gaacagcaga tacgcaaact ggcacagaag cacgcgcaga
    10021 tagagattaa aggccccttg ctggtactga taaattagcc ggcatttgta gtaaccattg
    10081 gctgtattaa aggattttag aaaacatcat ggcaaaagaa gcaaaaaaac aagacccgca
    10141 acagcagcta gaagcactgt tcaataaact ggtcaaacag cactccgagt ttactcagcc
    10201 attggtcgaa ttacccgagc atgaattcgc caaggctggt ctcattcaag gcctggttga
    10261 aggcaactta acgccaccag tcatgttgat tgcgctgaca gaaggccatt gggaatacgc
    10321 cgctgacact gcaaagtttg atgatttagc ctatcagctg ttaagtgata tcgaaggtga
    10381 ctggccagta tatgccgttg tggacgatgg cttgaaccac cgtattttgg ccttgttcgg
    10441 tgccgatgga tctgacggta cccacggtgc agataccctg cctggtctgg aagaattacg
    10501 tagctttgat cgcatggagc gtgatccaac ctttcgctgg tcgatgcgtg tttatacacg
    10561 cttaatgcag cgatttgatg ccttccatga aaatgtttat cgcgttacca aagacaaggt
    10621 aaacgacaag aacgacatta ttgaggaagt agctaagctt ctgtttttag aaactttccg
    10681 cctgcatcac gatgaagacc ttacctttaa agatgatgag ggaaataccc tgcgttttcg
    10741 tgatgtcttt aattggcaat atgtggagag ccacggcgat aaagccgttg cccaaatcaa
    10801 agccgccttt gaggaattta aaagccacga aaactacgtt gtgatctcag atgatggctc
    10861 acgtaacccc atctttagta aagaaacaca cctgcggctg tctgttgcaa aaaactatca
    10921 ggatttgctg gaagcaatac aaaacctggg accggtaaaa accaacgacg gtaaaatcgc
    10981 taaagaacac ggcacgttgg ctgatgtgtc aggagattta ctcggcagag tgtttgatgt
    11041 attcctacgc gctaactttg aatccaaagg tggccttggc gtttatttaa cacctaaccc
    11101 agtaaaacag gccatgctag aaattgctat gcacgacatc gacgatgacg atgaaatgcg
    11161 atcgcgctta gccaatggcg attttcgttt ctgcgacccc acctgtggct cctttggttt
    11221 tggctcggtg gcgttaagcc agattgataa atggatcgat tttaagctgg tgctggccga
    11281 cgataaaaaa gaatcattga aacaaaaatt acgcgactgt gcatttaccg gtgccgatgc
    11341 tgcaccacgc atggtaatgc tggcccgcgt gaatatggca ttgcaaggtg cccccaaagc
    11401 acagattttc tataccgaca actcactcac cactaacgcc ctgaagccga atagctttga
    11461 ccttatttgc accaacccgc cgttcggtac gcctaaattt gataaaggca aaaatggtca
    11521 aaactcaaaa gccaattacg aagccaatat ggagcaagtg ctcggtggtt ttcgtccgac
    11581 caaaaaagtc gttgatagct acaacgcctg gtatgaccac gtaaaaatga agtgggagga
    11641 tatcggtgat ttagagctgg atgaaaacgg cgagcctaaa tgggcgggct atcgtaccga
    11701 tttgcgcaga acgggcggga ctgataaaaa gccgttttac agcctgcagc ctacaaccgc
    11761 agggctcgcg ctgggcagca agcccgatag caagggcaac tggcaaccgg taggtgccac
    11821 tattgaccca gcagtacttt ttatcgaccg ctgcctgcaa ctgcttaaac ccggaggccg
    11881 tctgttaata gtgctgcccg atggtatttt atgtaactcc ggcgaccgtt atgtacgcga
    11941 gtacatcatg gggaaaaaag acgaaaaaac cggcgagttt gttggcggta aagccattgt
    12001 aaaagcagta atttcattgc cgagcgactg ctttaagctc tctggcacgg gcgccaaaac
    12061 ctcgattttg tatttgcaaa aacgccatgc taaccccaac cagccagagc aatttttgcc
    12121 cgagccacaa actgacgtgt ttatggcagt ggctgaaacc ttgggctacg tggtgaaaaa
    12181 taatattgaa gactataacg ctggtgtcgc aaacgatctg gataagattg tatcggctta
    12241 taagcggggt gagtgatgga caaatttttt cgaacaaaca gctcaattct tgtggaaaga
    12301 cttgatccta acttttatca accgagccta gtagaaaatg acaaactgat aaaatccaaa
    12361 ggctgggtgg aactccagtc actttatgaa gcgagtggta ttggaaacac gtcagcagtc
    12421 gaacggtatt actctgaagc tgctgatagt attccatata tttcgggaaa ggtaattaaa
    12481 agcttcaata ttgatttaga tgaatgccaa cgaatatctc tcgattcaca taaaaatgag
    12541 cttacaaaat cagcgctcaa accaacagat gttttagtta ttagaaaagg agatatggga
    12601 aatgcgtgcg tagttccatc tgaagttaac gaagcaaact gttcttcaga agtcatatac
    12661 ctgaaaatga aggcctctag cgatccttac tatctcgtta gctacctgaa ctgtgaccaa
    12721 ggacaaaagg cttttaagcg actaggccgg gggactatta ttcctggagt gagcttgtta
    12781 gatgttccac gtttacctat tccgaaagtg tctgagtttg ttcaaaaata catcggggat
    12841 aaagttcgcc aagccgagca gctgcgcgct tgggcgaagc tgcttcgcac atcagttgac
    12901 gctcatctta atagtctaaa tctgccgatc aacgagcccc ccgcactatt aaaccgagtc
    12961 agcgcgcaaa caatggagga tcgactcgat ccacgtcctt atcggactca ctacctatgt
    13021 ttagtgcgtg aaattgaaaa actacctcat gacagcatta gcactttggt tgaattggca
    13081 tctggttgcc cagtatccag taacgacttt ttggaaaact caggcatacc tttagttcgc
    13141 atccgcaata tcggttttga tgattttatt ggtttagata ctggcgttag ccaagatgta
    13201 tatcaggatg cgactaagta tcaagctaaa gacaaaatga tcgttgtcgg tatggacggt
    13261 attttccgct cgcagttttt catatctgac gagctaccta tgctggttaa tcaacgggtt
    13321 gcaatgctct cacctcaaaa tattcgtggc gaactattaa cacattggct aaatcgcccc
    13381 gaaggacaaa tgcagctaaa tcaatgggca gttaaaacaa ctgtagaaca tacttcccta
    13441 tctgatattg gtcgcgtact aattcctcga ctggataaaa gcttagaaaa caagcttgct
    13501 gattatttgc tcaatgcgag gctggcttat cgttatgcca aatttttaac acaagtagcc
    13561 aaaaccctcg tcgaagccct catcgaaggt caactgaccg agcaacaatt aatccaggct
    13621 caacaagccc tggaagatgg cgacaacagc tttgatcaag cgattttaag taaactgagc
    13681 gctgagggtt atgccataga aggcgccaca ccgctattta gcgatgtgga tgagttatac
    13741 agtttgttgg aagaggcggc gcaagcggag gctgaagagt aaagatggag acaaggggca
    13801 tggacagcat tacaaccagg caaaataacg accccgtaaa tagcgaagcg gcacttaacc
    13861 tctgcctgca gctttggcat caaggcggtt taaccgccag taaagcagcg ttgttactgg
    13921 cggctgtgcc tgccctcagg tcactgttac aaccaataat acagccgaaa aagaaagacg
    13981 ctgaaacgga tatcattagc gcctttagcc tcactgcacc attacttgat gcctttagtg
    14041 atttaagcca gtcgggtgaa tggcaattag cgttgttggg cttaaaccca gatgttcgcc
    14101 aacattggat aaatttagcc gccgctcgct gccaggaagc tggcgcgatg agtgacccaa
    14161 tggtattggt taaactcatt cagcaactgg gcaatgccag tgaatgggtc ttagcccagt
    14221 tggaaaacgc agacttctca ccacaaatca ttgcaagccc attggcgcaa accgaacggg
    14281 atttactcgg tcatagccta aacgacaatg ctgccattcc cgctctatgc cgtattttgc
    14341 gcaccagcca taccctgttt accgtgagcg aacaaaatga accaccggca tctatacaag
    14401 cggtggatgt caccgcaaaa caactgacaa ataactggtg tagtgggcgg ttgttagcac
    14461 tccctaatac cttgcttgat gaacataacc ttaaacccaa tgccgattgg ttattggtgt
    14521 cccgctcggg tcatgacaac gtgccgctta ccgagctatt cgcccaacag ccctggttat
    14581 ttttactcag tctaattatc tttgtgcaag atgcctgggc tgccgagcaa cgtggcggtt
    14641 tgttgctcac cttacctgcc gggcaaaatg cctttgcccc cggtcaaata aatgtggcgg
    14701 tacagggcat tgaaggtgat gaggtgagtc tgggtagttt ggccgagttt cttgtgcttc
    14761 tgctcggtga gctcaatatc cccctctatc cggcactcga tgcgaacacg gaaagcatca
    14821 accgcctaaa ccgcgtcctg agctcattca ttgccgaatt gttggcaaaa aaaatctggc
    14881 aatttaccga ggcgggtcgt ggtgagtccg gccaatatcg aattcataca agcttcagcg
    14941 atgcctgtta tagcctgccg ttagcaccac tatttggcta caaaagccaa acgttacaac
    15001 gggccattaa gcaactggcg caaaactgct atgccaataa aaaacgcgcc gccaatagaa
    15061 taaaccttca aggatcgtcg ttatgagttt tgaattaaac gaacagatgg ttgatagcct
    15121 ggcaaaacaa catcgctggg cggcgacggg cgtatcagat tatccacctc gcgacccgtt
    15181 agtgggccag agccgctttt ttaaacgctt tcaaaccttt ttgcatacgg tggatcacga
    15241 cgatgatcgc tttgcccatg tgtttgccgt cgaagccgaa tggggtcggg gtaaatcacg
    15301 tttaggccat gaattggtcg cacaaattaa cgactgttca aggggctggt ttgtccgcga
    15361 cgaacaaggc caactgcacg ataagcagct gtttaatcgc gacacgcaag acaaatacct
    15421 ggcgctctac attcgctact cgcaagtggc atcagattat caaaactcgg ataactggtt
    15481 tgcttttggt ttatatcagg cgctattacc actggccacc caaacctttg atggatcgat
    15541 tcaaagtgaa attgccaaac aagcctatga acgcttaatg ccatttggct ttaaatctga
    15601 actgttagca gatgccttac agctgaacgc caaccacagc gaagaagacc tctatactga
    15661 ccaaacgctg gtagtcagtt tggtgcaagc cgcctataaa gtattaaaga gctttggcgt
    15721 gcagtatatg ttggtggtgc tggatgagct ggaaaccgtt gcagaagccg ccacctttgg
    15781 cctggaagat gaacaaaata aacgcctcga tggccaggcc attcgcctga ttggtaaagc
    15841 aattaaagag gaagaccctc gccgtaaact tccatggttg cgctatgtag cgctgtgttc
    15901 accactgctt ggccagcaat tgcgcgaaat tcaatcggtc gcacgccgct ttgagttagt
    15961 ggaacttgag cataatgcct ttgccgatgt atcagactat gtggcaagcc tcttaaaaga
    16021 cggcaagctg ggttttaatt accctgctgg tttagtggaa gctgcatacg ccatgagtgg
    16081 tgccaacttc ggttggttta acgtggtcat ggctaatgtg gatgcagtgc tgagccaata
    16141 ccaacaagca ggccgcagta tcaaggatgt gggcgattta tttgaagctg tgctggcagg
    16201 ctctggccgc gtagccaggc atgtgctgga taaaggcgcg atagaaggca ttcaaactcg
    16261 cgaccaggca ctattgaccg aatgtcgtag tttgctctac ggccaattac ccgtgcgctt
    16321 agctgaaatg gcctatgcca cagaattgct ggcgctgaaa aacgaagatc tggaacccat
    16381 agccgctcat taccgcaaac tgagcattga ccgcctacag tgccgacaag ccctggagca
    16441 agccaaattc cgccgcgata aagatgaatg gtactaccct gcagtagatc aagcgctgaa
    16501 cttagatact ttgctggata acctgcgtac tttttcaatt aaagaacaag ccgcaagcgg
    16561 cgataaaggc gcggtattac tacctctgtc aaaaggtgag tttaaatact tactcagctt
    16621 gctgtatgac cacccagcga ttgaattcgc tgccgatgcc ttatggcaca agctagtggg
    16681 tgaaactacc gaactggacg ccagcgaagc aacgcatatt ggccccagtg tggcgatgtt
    16741 actgcgcctg gatttgcgct atcgcagtgc ccagcaaaac tcactgattt ttaaagatcc
    16801 cgccatgggc gacgctcatg aacaggcaat gagtgaactt agcaaacaaa gtagccagaa
    16861 gccgctcatc aaggcattag cccgtgtaac gggctttgtg agattacttg ataacaactg
    16921 gacctatgat gaaaacttgc tgagtaataa agcgggcagc gacgatgcac ctttggccat
    16981 tttaaccgag ccccgtggcc gccagggtgg tttacttacc ctggatggat ttaagcttca
    17041 cccagaaggt aaagccttgt ttgcctgggt cagcaaccta attgagctga acaatcttca
    17101 cacctttgcg gcaaaccaca gccaccaaaa tggtcgcacg ccagtattag cgttaactgc
    17161 ttctgcacat ctcatggagc aatacagccg actggatgaa agaaatgagt tgcgtgatgc
    17221 gatactgctg cattatgtca acccaagcga agcagaccag ttagagcgta ttggcttaag
    17281 tttggcagca tgtcagttgc acggcgtgaa tttaactgcc gacagcttta ccgcgaaatt
    17341 caaaaacaag ctgcatgccc tgacaacctt tgccacggat gccattcaca agtggcgcca
    17401 gcgtttgcag cagcgcggtt taattgcctg gccgttaaag gtagatggta aattatcacc
    17461 gaatgaccga gacctgttgt ttaaaggctg gtatcagctg gccattgctc accctgagtt
    17521 aaatggcatt ctcgatttac agcagcaaca cggcgtgcca gtgaatgagc tgagcagctt
    17581 gttggataaa cttaaggtgc cgggcagcta tattgccaaa ggttacactg ccgatgaaca
    17641 tgccgggcta tttactgaac ttaataatgt gcagcgaagc caggcgcaga tcccattatt
    17701 tttagcccgt attgctcacc ctaataaaaa gcacaagtgg caattcgaag gctttaagca
    17761 acagttttat ttcgcctatg tggctgaaac gagtgttacc gccaaaggcg tgtttaatga
    17821 ctggatgtgg tggtgtggag agctgaactt actcacttta accaatccaa cagaaaaaca
    17881 ggcaatttgg gagcactacc cgcgctcccg cttagaaaat gccattagag aagcgcaaaa
    17941 ctggttccgt ggtaacgata tgggcagcta tgcagccaat gtggaagtca tgagccgcgt
    18001 gtatggttat gcacgcatta atgaaatgtt cgcgccactg ggtaaaaata agctgggttt
    18061 cgtcaccgtg gaagctaaag agcagctgga aaaagcacaa tcgctattta acgtgctcaa
    18121 acagcaagaa gaacaattgg ctgatatggt cgaggccaac gacacgaaag tattggcagg
    18181 gcttatccac aaacgcgctg aagtgctgga gctggtagca aaggttaaac cgcttaatag
    18241 ttccaggcct atgctaaaag atgcccatat tctcagctta gaagacaaaa cgacctcgct
    18301 ttatcaacgg attgaacagg cctgtttatt tgccgagttt gttgagtgca gcgctgagcg
    18361 tatcaacaac cgactcgttg atttaatcat agatgttgaa acagagtgtg ccccgctgac
    18421 gaattttccg aaacgtctgt acaccaacac gctgcgcacc ataggtcata ttttagatgg
    18481 agcgttaaaa gacgatacca gcagtgccac tggccgaaaa gagcagcaag cagatagcga
    18541 taccttactg cattacctgc gtaaactcga tttaggtaga gcgcatgaca agctttcagc
    18601 cttagcgcaa gaagtgggtt taaacctgca caatgatcaa cagttgccga tagcggagat
    18661 tcaggggcat attttgtcca gctaccgcaa ctgcaaagaa cgatttagta agctggttaa
    18721 taacctcaca gagcaaaaac tacgtgcaca acaactgcaa gaatggctta gtagcgccac
    18781 ggctgagtat cagttcgccg atgacattgc agagctacca aaactggtaa tgaaattaca
    18841 gctcattgaa gatgctattg ccgatctccc cagtgatgta gagagcaagc gtcaaagtat
    18901 gcaaaacagt ttgcgcaatg ggcagttttc atcgttacgg gatttaccag aagagctttt
    18961 aaaaccggca cgcggtcaac ttacaccgat tcaaggccag ctacttaaaa ttgaagagcg
    19021 tctaaaccaa gtaagacgca actccatcgc acaggtaaac agctggctgc cgttattaaa
    19081 accgctattg gcttctcaaa agcaagcgga accagcagcg ttgactctgg aggatgtgag
    19141 caatttgggt gtcagagaac ttcaacaggt gtgtgaaagc acccaggcca aatggcagag
    19201 tcaaggtgag caaatattaa atgatacagg cttaacactg gcggattggc aaccgattta
    19261 taaggctttg agccaaaatc aggaacccgt gttaacacct gagcaacaga aaggacttgt
    19321 agacaaaggc attgtaaaaa tgcgccttac ctttgccact ggtttgtaga ggtgacgcga
    19381 tgagtcgtta tttatctgcg gcactggcca gtaatcgtaa aggccgtttt ttgcaaactg
    19441 ttgcgggggc taccccctta atgaaggatt ggattagctc gctgccagcc agcggtttgt
    19501 taatagtgca agcagaagag ttgactgacg ctaatactat gcagcacctg tatcactggg
    19561 ctatgcaggc aggttgtgcc gctttggtaa taaacctgaa agcagaacaa tttaccttat
    19621 tagcgcaatt gccctatcca ctggattggc agttagtacc agcgagcttg agggggcagg
    19681 aaccggggct aaccgctttg ctagccagtg aaacagacca ggctattgct ggctttactg
    19741 gcagcgcaga tcgctaccag caccaggccg gtgatgtagt gcatacccgc tacatacgaa
    19801 aacacagcaa cagtgggcta ttagccttta ccacgttacc tttgtggtcg ctgactttac
    19861 tcgatcatag tgagctcctg gtgagctggt tgaattggtt tgtggaccat gcaggcattg
    19921 ctgaacgaat aatcgaacct aaagcgccat caactgacta cacgccagat aagcacgatc
    19981 tggttgtatt gctattactc tacgctggcg gtggcatgaa cttacaagcg ttgtctgagc
    20041 ataatgcggt taagctgatg tttgatgtta atagccttga tattgtcaag cggggtgaga
    20101 tgttgcggca gcacgatttt atcgacgacg cgggcataac agctaccggt aaaacatgcc
    20161 tacaagcctc gcaatattgg gcttatgccc cattacttgg cgagcagcta cacacaggaa
    20221 cgctataagc atgaatgaga acaaaacgat gaatttagcg caatcgctcg ccatcgatag
    20281 ccagcttcgc ttaccgggaa gtgcaggacg cttgcttaaa caggtccgtg atattgagca
    20341 agtggcgccg ctattccgtg atgctggctt agtgaaagcg gtccccaagc ccacaattgt
    20401 taatttacgc gtagccggta ttgcgactaa atcgtgctta gacaaggtat tgggtggttt
    20461 tatttatgcc agtgcagcag tgcgcacaga tttgttaata gatgacaata aagtcagcac
    20521 ggtgggtagc gactcggtat cagaactgga tgatattgat tttgaagctc agcgtaaacg
    20581 gcttgattta attgaaattc gtcaggctta tcagttagtt gagaaaaaac ttttaggtta
    20641 tgagcctggc gatttaattt tgctggatac gccgttgttt ttagcacggg atatggctcc
    20701 gctggagaga aatgtccgtc ataaacaaga gtacgaaaaa accagacaag tgatagaaac
    20761 tttttggcaa aaccatcgct ccagaatttt tccctggaat caaaatggtg tagtacttgc
    20821 gagcattttg gctgagcgct ttagtgccat tgtaagtatc gccaaacaag atttacgtac
    20881 cgaacaagga cgcaaacaaa ttttagcatc tgatgggttt tctaaagagt tgatgcaaaa
    20941 tctggaagga ctggatgaaa gtttggttgg cgtgggagat ttgcgattta tcaatggcat
    21001 tttaggcaac tattcgcgca ccatcgcatt tcgtttgtct gaacaagaaa agcgcattga
    21061 acccaatatt gaagctaagc aaggtctaat tggttttcac ttccgtagtg cccgaggtgg
    21121 acagataaaa atggtgcaat tagcgggtga cgaaccagaa tggaaaagtg ctgatctcga
    21181 tttagttgcc tctcgcctga tggtgctgga tatgcaaaac aaaggtaatg caatgccgtt
    21241 gccacaatta cttggttatc aacagcttgg tattttacct aaatttgcta cgttttatcg
    21301 ccagggttta caccaggcac taaagaataa tgatattgag gcagggtggt tggcaggcct
    21361 cgatgaggga ttgaataatg gcttataaag ttttaggtaa attagtcggt aacactggtg
    21421 atgccagtca gttaactatg gttgtacaag actcgttttc agtacgccgt ggtgagtttg
    21481 tacgcattat gcaccaggaa cgcaaagatg aggggcaagt cgccgtactg gggcgagtga
    21541 ccaagataag ccgtactaat atgctctata acgccggctt tggcgatggt gtaaccgagc
    21601 ttgagctttt gcccggtgcg aatgtaactg gtgagaatat gtttgctcag gtcgagctgg
    21661 ttggttaccg cgatcctgtt actcgtcaaa tcaaaattcc acgtcgcccg cttaacccgg
    21721 gtaccgctgt tgaaaccgtc gattttcagt ttttgagtga tttttacgaa tttaatgaac
    21781 actccagttt gcacttaggt aacttagtag gctacgacaa aggtgagaat acagttcctg
    21841 tttttattga cgtgaacaag ttggtcactg agcatttggc tgttctggca atgacgggtt
    21901 caggtaagtc ttatacagtt ggccgcatta ttgagcgttt agttgcggtt aataacggta
    21961 cggtagtagt atttgaccca catggtgaat acggtaaagc cctcgctggc ggtaatctgc
    22021 aattctcaag cttcttagaa gccactgacg ataagcgtga tcaagaagca ctgccactga
    22081 taaaacagac ctttgaaaag ctgcaagccg ccggtgcagg tattcaggtt tactcgccgc
    22141 agcatgaatc ttttaaacat aaatacgcca gtaagaatac gccattagcg cttcagtttg
    22201 accattttga aatggacgat attgctgaaa tattacctgg cttaacggaa ccgcagcaac
    22261 gagtactcga tgttgcaatt cgttattggc gctatgcaga taagactgag ccacgagata
    22321 ttaaccgcct acgcttctta ttaggtgatg gtattgaaga gttaaaagag tgggacgacc
    22381 tttctgaagc tgaggcgaag gcgttatcag gccgtagtgc cgccgtcgcc tcgatgaagc
    22441 tttcacgtgt gcttaatgaa gctaaaagtt tttacagcgc aacaatggct gagccaactg
    22501 atatttacaa aatggtgggt cgcccgagca atcaacaggg gcgtttagtc gttgttgact
    22561 tacaaggttt gagtgatacc gctaagcaag tcatttgtgc actgttatct agtgaaatat
    22621 taaaagcagc ctcgagcaaa acggacccgc tgcgcccatg ctttatcatt tacgaagagg
    22681 gtcataattt tgccccggcc ggcggcaacg cagtcagtca ccgcattatt aaaaagatcg
    22741 ctggtgaagg tcgtaaattt ggtgttggat ttggcattgt gagccagcgt ccatcgaaat
    22801 tagattcaga tgtgacctct cagtgcaata cgctaattac gatgcgctta aagaaccctg
    22861 acgatcaacg atttatagct aaagcatcag atatggtgag caaagctgat ctggaggaac
    22921 tcccgagttt atcaaccggt gaagctttgg tgtgtggtcg ttcaatccct gcaccactgc
    22981 ttgttaaagt tggtagtaag gcattaattc acggtggtga atcaccagag gtactgcgtg
    23041 tttggggctc tttcagtggc taaggtgcct atgcctgaag ttgcctgggt aaatgagaca
    23101 tttccacttt tagcaagcgg taagctattg gaccaggatg tattggtcca cagcctgggc
    23161 tgtagcgttt ggaatacatt cgggcatgaa cagtcattta tggcggtagt ggaatgcccg
    23221 gctccaggga catttggtgc tgatattcgc tctgactcgg gctggtttca taaatcttct
    23281 gcatcgcctg tgtgtttaat cgaatttgaa cgctttgatg gttcagcgaa ggggcagcaa
    23341 aagctggaag aaaaattaaa aaacttactg gaagcagcac agcgctggaa tcattgcccc
    23401 aaaaccctgg tcctcagtgc ctggagccag ggcttagtag gtgtacctga tactcaaaaa
    23461 ctgaaagaca tatgccgtat gggttttact tcgtccacgg gtacccaggt aatcgccgct
    23521 cctgatgttg aggtggtatt tagtcgcttt ttatttatca aaaacctgaa catgattgtt
    23581 ttggacagaa ttcactatga ggtgttgatg tgaatataaa atcagcacat tttttgccag
    23641 ggacgaataa acgccttggt gacaaatacc tgttacttga atgccttggt gatggcagcc
    23701 atggctgggt atggcgggcg gaacgcctga aagatggaaa aattgtcgca gtaaaaatcc
    23761 ctaaggatat tactcgggaa gaccgtcagc ttgccgaagg taaagagctt ttaaacgttg
    23821 agccgcacga aaatattgtg caaatttttg atatgggccg tatagataat gaatggttct
    23881 atattgaaat ggagtatttt cccagccaaa ccctggcgca aaagctcgat gacaggcagc
    23941 gtaattttgg ccagacctat gaacggttgt tcagaatata tcgtcaagtg ctctgtgctg
    24001 tgcattattt ggccgaatta ccagtaccaa tatctcatgg agatattaag ccacataaca
    24061 ttttggttgg agagcgggac ctggtgaagt tgacggactt tggcagctca gctttaccag
    24121 aagaaattta cgttcgtacc cgtgaaaatg gcgggactgt gctgtattcg gccccagagt
    24181 tctccaacgt cgatagccgt agaggcagcc tggaagagct actattgggc gacatctata
    24241 gcttaggtgt tttgctatac cagctgctga caggaaagct gccccatgat acgccagcac
    24301 aagttcagcg ccacgcacct ttcaagcttc cgacagagat taatagctct attcatacgg
    24361 acctggaaca agtggttctt acttgtttac aaaaacgagc ggaggatcgc ttttccacta
    24421 tttccgactt aattcatgca tttgattctg ctgccactaa gcaattaaaa gttggtgcta
    24481 ttgctcccgt ttttcaaact aaacctgctc aggactggtc aagtgcggta cttgaagcaa
    24541 tgtccgatca atcttaccaa aaagctgctc aattagcgtc gcaggagtat agccgcagta
    24601 aagatctaca agctttacat cagcaactta ttgccttgta tcgcgccaat cgcttattcg
    24661 actttgaaaa agttgtagaa gataataaag cgatcttact tgagggtaaa ttgactcaac
    24721 cagcagcttt gttcgagttg atagttaagg caaacctaca gttacgcaat atcagtcaag
    24781 caaatttctg gctgttgcaa cgaaagcaag ctgaagcaga aaacgcggcc accatgtatt
    24841 tagaatcatc tatccttggc cttgaagcga aatattctga agcaagagta ctattggaga
    24901 aagtaaacca aacaactcca atgaagttcc atgtgttaag ccaactcatt ttagtttgcg
    24961 agcaaatgcg tgattactct ggtgcagcgg cttatttgaa agcggcattg cgagtagcac
    25021 cattggataa cagcatgaaa gagaaaaaag aattgtatgc aaagcttgga gttatttgat
    25081 tgaaaggatg accattgcgc gaacaaggca tagtggatat ttaacacttc cgttcacata
    25141 ccggacaaaa aaataaagtt ttacataacg atttccttct tgattgagtt aagacaaaag
    25201 cctctcccca ttagggcgga ggcttctcag ttataagcct ttaattaaaa aaagttaata
    25261 ttcatatact tagctagaat tatcgaaaag aactattaat ctttgcatag tcaaatggag
    25321 tgatagccgc cattctattc gcatccagat aatcagccca ccactgcagc atcaacattc
    25381 gctccccaag atgttccgcc ttatggatat aagccgcccg tactgagcta cgttcctgat
    25441 gactcatctg ccgctctact gcatccctcg accacaatcc cgattcaatc aaagaactac
    25501 acgccatcgt cctgaaacca tgtccacaca cttccgtttt cgtgtcatag cccatcactc
    25561 gcaaggcctt gttaagcgta ttctcactca ttggcttacg cggatcgtga tcaccaacga
    25621 agatcagctc acggtttcca ctcatgcttt tgattttttc cagaatggtg agagcttgtc
    25681 gagataaggg tacaagatgt ggagtccgca tcttcgaacc acgctgagaa tgcttaactc
    25741 cctccagtgg ctcacgttct cccggaatcg tccacatggc agtatcaaaa tcaacttctg
    25801 accagcgagc aaaacgcaac tcgcttgaac ggatgaaaac taacaatgtg agttcaaccg
    25861 ccagtcgggt taacggcctg ccagaatagt gatagatgcg ttgaagcaat tcaggaatgc
    25921 ggttaagttc cagagcagca cgatgctgtc ttttcgccgt agcaaccgca ccagcaatct
    25981 cttgagcggg gttgtagtcg attaatccac tctgtacagc aaagcgtata atcgcggtag
    26041 tatgctgctg caaacgagca gcgacttcga gacgcccaga cgactccacc gctttgatgg
    26101 ggacaagcag atcccgagtc ttcagatcag caatgttccg cttaccgata gcagcaaaga
    26161 gattatcttc gaggcttttc aatacacgag cgctatgcga tgcagaccac ttctgattgc
    26221 tggcgtgcca ctctctggct accacttcaa atgttatcac ctcttgctca tgctctacct
    26281 taacagcttt cttgttctca ctaggatcga cgccgttagc taacaactta cgggcttcat
    26341 cccggcgacc tctggcatcg accagcgaga cttcagggta tttccccagc gccagcatct
    26401 tctccttacc gccaaaacga taacgaagtc gccagtattt ggagccatta gggtgaacca
    26461 acaaaaccat gccttcaccg tcagtaagtt tataggcttt tgcttccggc ttagccgaac
    26521 ggaccttcac atcactcaga gccatgatga gtatcctttc aagggccctg tgtgggtaca
    26581 aacataatcg aaccgggaaa tacccgcagt tgtacccgca tcagtaagtt gatgtagatt
    26641 gaatcaggtt gacttaggtt gagtgaaaaa gcgaggaaag ccttgcggat actggatttc
    26701 aggcataaaa aaagacctca gttgaggtct atttacatac ttttggtgcc gaaggccgga
    26761 ctcgaaccgg cacgatttta acggtcgttc gcttgcctag caagctcctg taatgctttg
    26821 aatcaaaagc aaaactaaca acacatcgac acttcacact gtacactatc aagtgcaaaa
    26881 gccctgacaa aatagccggg gtttgtatga gtttcaagag agatcgttat ttaatttcag
    26941 attcttgcgg aaactatctg gtaaggatcg ctatacctaa gtatatgcgt catctatttg
    27001 gtgggcggtg tagctacgtt aaaagcacag gcacaagaga tattcgagaa gcacggttgt
    27061 tccgtgatgc ggtggcgctg gaatggataa aactacgtaa caagctaaag cctcaacgtg
    27121 agaaaacatc gaagatcgag caaattatta cagaactacg gaaagctgga acatatgcat
    27181 ttcaagtatc tgaatcattt aatgattctt ctattggctg cccttctctt ttaaatatgc
    27241 gggatcagta cattctccag tttagcgaga agcgcaagct aactacgcta tcaaagacaa
    27301 acaaagccgt agaggtgctc ttagaacatc tgaataaacg cgatgttcag cttcgtgacg
    27361 ttaaccgaac tattgttact agctggttag ataacctaaa aaatgagaaa gcaccacaaa
    27421 caatacaaaa ctacattagt gcattagccc agatttggga tcttgctcgt aaccgctacc
    27481 acgatgcacc acaagataac ccttggcgag gtcacggcct tgaagctaaa agtagcaagg
    27541 tgaattatga agcctttagc aatgatgaac tcgcaaaagt gttctcaatg ctggctgatg
    27601 aagaagaaat gcaaaatata acactgatcg gaatgtatac aggtatgcgg atcaatgaga
    27661 tagcaagtct cacaatagac gatgtgaaag agatcgaagg tgtgctgtgt tttgaaatca
    27721 cacagggaaa aacgaaagct gcggcacgtg ttgtgcctgt acatagcctt atcactccgt
    27781 tggtgttgtc gctgcgtgaa aagcctcata atggcttttt gttctatcac gccagcatta
    27841 cagagcgtgc tgatggtaaa cgctctacgt ggcacacgca gagatttaca agagctaaac
    27901 gaaaggcttt aggggaaaag ggaacagaaa ggaaagtgtt tcattctctg agacacggag
    27961 tagcacagct tcttgatcga aatcaaattc cagaagacag gatcgccctt ctcctgggcc
    28021 atacacgcgg caatacagag acattccgca catatagcaa gaatgcagct tctccagtag
    28081 agcttaaaaa ctatattgag cttctacgat accctgaaat agagaaaggc ttatcaatca
    28141 ataaaaaatc aaatttaagg cgtaaaacag cgccatagac gcaataaaga ggcttcaaca
    28201 tattggtagc ctcttttata attacatcgt tacagagcgt tttagaggca tttacggcta
    28261 ttcctgttta aaggcaggag taatagtgtc ttcaccttca ctacttgatt tttcagtgtt
    28321 aacacgtttt ttcatattcg ccccgatgtt cttagatggc tgtgttttga tagctacagt
    28381 gtttacagtg ccatacgaaa cgagcctgat ctgctcaaag ctaagatcaa ataccaggga
    28441 ttcgcttgcc tcttctgtgt attcgatgcc tgttaaaaca acgttctcaa aagttttatt
    28501 ttctgtaaca agaagaaaag gctgtctact gtccataatc tgattcaagt agtctatagc
    28561 taatgatctt ctgttcccgt tcacccctgc actctttaac aagccaggat cgcttttaat
    28621 cggtgtttca gtgatccttc ctttaagaga aaacttattg tttttaattt gaacgtgatc
    28681 agacacagta gcaccatttt ctacggggta gcttgtaaca tctgccgaac gtctcacgtt
    28741 cacatcgtca acactatcaa aggccagtgc catataattt tcataaacat cgtttccaga
    28801 agatgccagc ccactggtaa caatagcaaa tccgttctct ccttttgttt tattagcttt
    28861 gctattttcg ttttttgtag agtttatatc ttgttctgct ttgtaaacag cctgaacgtg
    28921 ttgttctgga gtatttgctg cacgtatggt taaaattccc gccgttgcca taataatttt
    28981 ccttgtaatg ttatggcggg aattacccgc cgttagattg tcctgataag ctggttaatg
    29041 ttaatgattt caagaagcta tcccgacttg ctctcgctgt aaaatccaca taatcgctta
    29101 atcttgatgt gtccgaatta atagtaaggg gaacttctgt taccacttca aacgttggat
    29161 tcagattaaa cacaggagat aaattataag ccggagttgt cattgcaaga ttatttaaag
    29221 caccctgttt gagattggtt atcggtgctt cgctgactga catttttaca tctgacacag
    29281 aagaagactt atcatcgaaa agccatccct taatcgtatt aaagagagaa tgactattat
    29341 caggtgcagt tgttgcagtt ccagcgcctt tatattggtc aggaacgaga ttagaaagat
    29401 gctgtttcat cccttcattg cttaatggcg gtgcatcacc taaatttttc tgaatagcat
    29461 ctgtcttatc atcattacga ttaaaccagt ttacaatctt cataatggcc tcaaagagac
    29521 ttccagacgc ttcccctagc ttcgtaaaat acggagtgag attagaaagg tttttcataa
    29581 actcttccgt tacttttgga tcgagagatt tcataaagcc gtctacgaat gctacttgtc
    29641 ttgcatcaga aagctgtgaa agctgtctgt ttagttctct gaatttaacg atagattctt
    29701 gctgtgtttc tgttagccat tttccagagg ctttttgctg gttaatactt tcttgtacat
    29761 ctttaacgga acgttgccaa tattttgatg taagcatcaa atcatcgccc aggtcttcta
    29821 aacgttgtcc aatttgggag tctgaaaacc cttttttcat cattccatta acagccttag
    29881 aaattaatcc agccggatta tcagcaaatt ttttaaggtc gcttttgtta ataacaccga
    29941 tgtttagaag ttcattaata gcgttatcac cgcctttcca tttagatttt ccggtttgct
    30001 tatctttcac caattcagct tcactgtagg attttgcggc acgttcccta acatctttca
    30061 tctgatccag gtatttccta gccccttgat caccctggag gctatcgatc ccgtgtctat
    30121 aggcccattc tgtaatgtta tacatctggt taacgtctac accgcccagc ttggcccgtt
    30181 gaattagctc cccttgcgtc tctgcgttag cgaagctgct agtaacaaaa tcagaggctt
    30241 tgcttacagc catcacgcca gcagcagccg tagcgcctcc aaataacaga gcgcccatag
    30301 tggcggaatt ccctgatcct cctcccttaa cgggggctaa ttgagctttt ctgttgccat
    30361 taatcttacg ttgttgctgt tgtaggcgtt tcatttggct attcatacgt gcaagcgaga
    30421 tagccccttg ctcatattgt tttgctatct ctctagcctg tgcaatagct ttgtattgtt
    30481 ccgctacaga gagacggtgc attgcactga tagagcttga cacatcgagg agctttaatt
    30541 ctgctttctc cttacgtgct gctatttgtt gctgttgcct tgcctgtgct cttgctgcgg
    30601 cagcttgtgc tctttgattc tgtttttcgt ttttcgcttt ctgttgagca atcttatcta
    30661 cttcgctttg tgctttctta tagcctttag tgacattatc aaatcttaat ttagctggct
    30721 tgatgttttc tgcgaattcc tttaagcctg taatagagtc tttcgctttt ttcaagctgt
    30781 ctttatctac actaaatgtt actgtgttct taattttaga agtgttaatt tctaacattt
    30841 aaaatccccc ttattaatca tttaccacct tctttactta agaaatggtg tagagcctga
    30901 tcaatactct cgtttagtct gaacatacgg gcttttactc cttcataggg aacatctaaa
    30961 agagaacata gatatttcag atttaacaag cctctagaat cattagcatc taaatgtacg
    31021 tgtacaccat tttttgattt cagaatcact tccatatttt aatcacctct caaaatttaa
    31081 aaaataaata aaaaaaaggc gaagcgtgaa tacacgccac gcctctatta cacgttaaaa
    31141 tgttatttaa cagtgatttg aacagagaag ccaacgccgt ggtttacaac catctggcta
    31201 ttctctgtaa caacgttaac agcatcacca cgagaagatt catagctgta gctgtacacc
    31261 tcagcaggag ccgcattgat cagttcaaag gtgctggaag ccgccccgaa gatgttggca
    31321 taaacacctg cccccttagc aaacttcgga accgctacag cgtctgcggt catatgtgca
    31381 aggtgtagcg gatctaccag tttaataaca tcaatgttag ttcctggaat agtgaacgct
    31441 gtcacccccg gtagcaggtc acgacgctgg tacacaacat ttccctcttc cagtgggctt
    31501 acatactgga atgcgtttgc catacccgca gagaatcgaa ttgcagagaa ggcttccggc
    31561 ttacagaaaa caacaatacg ctctacagca gccgcctggc tttgtgcttt ctcgataatg
    31621 tcagtagcaa tctgatcaaa ttctttaaaa atcttcgtgg tgtcagtgct ggaagcgttc
    31681 acagtggctt tcatagctga tacgttaaac agcttgcccc actcgataag aacatcatca
    31741 gttttcgggg tgtaggtgcg accactaaac agagaagcag ccaatacgct ctcttttgta
    31801 cgctgatacg ctacgtggtg tttagcaaca tattccgcgt agatatcagt gagtgtttcc
    31861 tggaaatcag tgccaggttt acgtttgcct tgaacatcag aaggacggat cacatcttca
    31921 cgcaggaagt aagggatctc gatcagccat tctttacctt gttctcgctt agtaacgtta
    31981 tgctcagaag agaaacgaga ggtttctttg ttgaacagag attcgttatt ttcgacaagc
    32041 tgggaaacag ctactttatg agaactaacg cccaccggat cagtaaaatc cagagtagaa
    32101 agcagataat ttctagtgct atgcgcagag aacagagga
//
LOCUS       DAZFQD010000046        31962 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.046, whole genome shotgun sequence.
ACCESSION   DAZFQD010000046 DAZFQD010000000
VERSION     DAZFQD010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31962
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.046"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..203
                     /locus_tag="ACHKTW_002184"
     CDS             <1..203
                     /locus_tag="ACHKTW_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000274107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6463206.1"
                     /translation="ILVLFILSLTTTLNLRSIGIGLSFGFCQILGRYTMSLGWSKYWG
                     VLGVFPVTNIVLFLILIFFKKR"
     gene            complement(250..1137)
                     /gene="rarD"
                     /locus_tag="ACHKTW_002185"
     CDS             complement(250..1137)
                     /gene="rarD"
                     /locus_tag="ACHKTW_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HFO6463207.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAISAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFAFI
                     WVALAIFVMDAIYTQRRKS"
     gene            complement(1189..1656)
                     /gene="yigI"
                     /locus_tag="ACHKTW_002186"
     CDS             complement(1189..1656)
                     /gene="yigI"
                     /locus_tag="ACHKTW_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase YigI"
                     /protein_id="HFO6463208.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            1821..2690
                     /gene="pldA"
                     /locus_tag="ACHKTW_002187"
     CDS             1821..2690
                     /gene="pldA"
                     /locus_tag="ACHKTW_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004620 - phospholipase activity [Evidence
                     IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HFO6463209.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYRFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            2823..4652
                     /gene="recQ"
                     /locus_tag="ACHKTW_002188"
     CDS             2823..4652
                     /gene="recQ"
                     /locus_tag="ACHKTW_002188"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HFO6463210.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            4716..5336
                     /gene="rhtC"
                     /locus_tag="ACHKTW_002189"
     CDS             4716..5336
                     /gene="rhtC"
                     /locus_tag="ACHKTW_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HFO6463211.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEAVSAP
                     APQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTARWGIFALIIVET
                     LAWFTVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(5398..6018)
                     /gene="rhtB"
                     /locus_tag="ACHKTW_002190"
     CDS             complement(5398..6018)
                     /gene="rhtB"
                     /locus_tag="ACHKTW_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HFO6463212.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            6129..7151
                     /gene="pldB"
                     /locus_tag="ACHKTW_002191"
     CDS             6129..7151
                     /gene="pldB"
                     /locus_tag="ACHKTW_002191"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HFO6463213.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATPTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            7159..7959
                     /gene="yigL"
                     /locus_tag="ACHKTW_002192"
     CDS             7159..7959
                     /gene="yigL"
                     /locus_tag="ACHKTW_002192"
                     /EC_number="3.1.3.23"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HFO6463214.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGVSKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            8035..8934
                     /gene="bioP"
                     /locus_tag="ACHKTW_002193"
     CDS             8035..8934
                     /gene="bioP"
                     /locus_tag="ACHKTW_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HFO6463215.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRGNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQHNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQLHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(8822..9775)
                     /gene="metR"
                     /locus_tag="ACHKTW_002194"
     CDS             complement(8822..9775)
                     /gene="metR"
                     /locus_tag="ACHKTW_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HFO6463216.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     VTEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            9893..12154
                     /gene="metE"
                     /locus_tag="ACHKTW_002195"
     CDS             9893..12154
                     /gene="metE"
                     /locus_tag="ACHKTW_002195"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFO6463217.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLTVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPPRHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALGHNVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDDVAEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIKQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDVSRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            complement(12193..13008)
                     /gene="ysgA"
                     /locus_tag="ACHKTW_002196"
     CDS             complement(12193..13008)
                     /gene="ysgA"
                     /locus_tag="ACHKTW_002196"
                     /EC_number="3.-.-.-"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HFO6463218.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGGKKSL"
     gene            13270..14031
                     /gene="udp"
                     /locus_tag="ACHKTW_002197"
     CDS             13270..14031
                     /gene="udp"
                     /locus_tag="ACHKTW_002197"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HFO6463219.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTASSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESHAVKIVVEAARRLL"
     gene            14172..15599
                     /gene="rmuC"
                     /locus_tag="ACHKTW_002198"
     CDS             14172..15599
                     /gene="rmuC"
                     /locus_tag="ACHKTW_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HFO6463220.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            15694..16449
                     /gene="ubiE"
                     /locus_tag="ACHKTW_002199"
     CDS             15694..16449
                     /gene="ubiE"
                     /locus_tag="ACHKTW_002199"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HFO6463221.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            16463..17068
                     /gene="ubiJ"
                     /locus_tag="ACHKTW_002200"
     CDS             16463..17068
                     /gene="ubiJ"
                     /locus_tag="ACHKTW_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HFO6463222.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLTALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            17065..18705
                     /gene="ubiB"
                     /locus_tag="ACHKTW_002201"
     CDS             17065..18705
                     /gene="ubiB"
                     /locus_tag="ACHKTW_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HFO6463223.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVAALEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            18784..19053
                     /gene="tatA"
                     /locus_tag="ACHKTW_002202"
     CDS             18784..19053
                     /gene="tatA"
                     /locus_tag="ACHKTW_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HFO6463224.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            19057..19572
                     /gene="tatB"
                     /locus_tag="ACHKTW_002203"
     CDS             19057..19572
                     /gene="tatB"
                     /locus_tag="ACHKTW_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HFO6463225.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            19575..20351
                     /gene="tatC"
                     /locus_tag="ACHKTW_002204"
     CDS             19575..20351
                     /gene="tatC"
                     /locus_tag="ACHKTW_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HFO6463226.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSPEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            20393..21175
                     /gene="tatD"
                     /locus_tag="ACHKTW_002205"
     CDS             20393..21175
                     /gene="tatD"
                     /locus_tag="ACHKTW_002205"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HFO6463227.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAAELNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVARGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(21172..21660)
                     /gene="rfaH"
                     /locus_tag="ACHKTW_002206"
     CDS             complement(21172..21660)
                     /gene="rfaH"
                     /locus_tag="ACHKTW_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HFO6463228.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTINATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPATPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            21827..23320
                     /gene="ubiD"
                     /locus_tag="ACHKTW_002207"
     CDS             21827..23320
                     /gene="ubiD"
                     /locus_tag="ACHKTW_002207"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HFO6463229.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IEQGETAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            23366..24067
                     /gene="fre"
                     /locus_tag="ACHKTW_002208"
     CDS             23366..24067
                     /gene="fre"
                     /locus_tag="ACHKTW_002208"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HFO6463230.1"
                     /translation="MTTLSCKVTSVEAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDIPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHP
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(24259..25422)
                     /gene="fadA"
                     /locus_tag="ACHKTW_002209"
     CDS             complement(24259..25422)
                     /gene="fadA"
                     /locus_tag="ACHKTW_002209"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HFO6463231.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDAQACLVGGVEHMGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSGAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVSGTVTAGTSSALSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCD
                     PSIMGYCPVPASKLALKKAGLSVSDIGVFEMNEAFAAQILPCIKDLGLMEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMERKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(25432..27621)
                     /gene="fadB"
                     /locus_tag="ACHKTW_002210"
     CDS             complement(25432..27621)
                     /gene="fadB"
                     /locus_tag="ACHKTW_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0036125 - fatty acid beta-oxidation
                     multienzyme complex [Evidence IEA];
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004165 - delta(3)-delta(2)-enoyl-CoA
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HFO6463232.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRQEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLVEGAKAVLRQAINGDLDWKAKRQPK
                     LEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDVVVEAVVENPKVKKAVLAETEQKVRPNTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSLLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDTIDALFDANRFGQKNGLGFWRYKEGSKGKPKKEEDAVVDDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEESIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            27811..29142
                     /gene="pepQ"
                     /locus_tag="ACHKTW_002211"
     CDS             27811..29142
                     /gene="pepQ"
                     /locus_tag="ACHKTW_002211"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HFO6463233.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERVLQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHAAVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRCTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            29142..29756
                     /gene="yigZ"
                     /locus_tag="ACHKTW_002212"
     CDS             29142..29756
                     /gene="yigZ"
                     /locus_tag="ACHKTW_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HFO6463234.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITMLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYHQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            29795..31246
                     /gene="trkH"
                     /locus_tag="ACHKTW_002213"
     CDS             29795..31246
                     /gene="trkH"
                     /locus_tag="ACHKTW_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HFO6463235.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            31258..31803
                     /gene="hemG"
                     /locus_tag="ACHKTW_002214"
     CDS             31258..31803
                     /gene="hemG"
                     /locus_tag="ACHKTW_002214"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HFO6463236.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQTDVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMIKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKPTLK"
BASE COUNT         7403 a         8125 c         8754 g         7680 t
ORIGIN      
        1 ccatccttgt tctatttatt ttaagcctga ctacgacatt aaatttaaga agtataggta
       61 tagggttaag ttttgggttt tgtcaaatcc ttggtagata tacgatgtct ctaggatgga
      121 gtaagtactg gggagttttg ggggtatttc ctgtaacaaa tattgttctt tttcttatct
      181 taattttttt caaaaaaaga taaataagtc agtgggtagg ctctttaaac ggcctaccca
      241 tgttaattct taactttttc tacgctgcgt ataaatcgca tccatcacaa aaattgccag
      301 cgccacccaa ataaaggcga aagtcaccat cttatcggca cccggttttt caccataaaa
      361 cgtcacagcc agcaggaaca tcagcgtcgg gccaatgtac tggaaaaagc ctaacgttga
      421 gagacgcaag cgcgtggcag cggcggtaaa acacaacagc ggtacggtag tgacaatacc
      481 ggcggcgatc agcagtaaat tcagcgacat cgggttttgc cccatatggc tggttgagct
      541 gtcggcaata gcaaacaggt aaattgccgc cacgggcagc agccacatgg tttcgattaa
      601 catgccggtt tgcgcttcaa cggcaatctt cttgcgtacc agaccgtaga aggcaaaact
      661 aaatgccagt cccagcgcga taataggtag cgaaccaaaa gtccacagct ggactaacac
      721 gccacatatc gccagaatca ccgccagcca ttgcatccgg cggaatcgct cgccgaggaa
      781 aatcatcccc agcacaatgt tcaccagcgg gttaataaag taaccaaggc tcgcttccag
      841 catatggtga ttgttcaccg cccagataaa cagtagccag ttgccaccaa tcagcacggc
      901 agagattgcc agcataaaaa ttttctgtgg cgtctgaatc agcgttttta aataggacca
      961 ctggcggcaa atgctcatca gcaccaccat aaagaaaaac gaccagatca cgcgatgcgt
     1021 caggatttca tcggcaggca cgtagtaaat caacttgaag tacgctggcg ctatacccca
     1081 aataaaataa gcggcaagag cgagtaatac gccctgccgc gtttgttttg catccatcgg
     1141 gaatactcat ttttaattgg taacagcagt ttacctgctt ttatgtcttc aacctaccat
     1201 ataggtggcg gtggcactgg caatataaag ctgttcttca ttgtgtaatt caacgcgggc
     1261 gacggcgact ttattgcctg cacgcaacag gctactggta gcagtaaaac gctcgcccct
     1321 gcctgggcgc agataatcaa cgcgaagatc aatggtcccc atccgcgata gccgctggcg
     1381 tagttcatct tcactgatgg tttcgtggcg ggttaaggta cttcccacgc acaccagacc
     1441 ggcggcgaca tccagcgccg acgcaatgac cccgccgtgc aaaatgcttt gcgcccagtt
     1501 gcccaccatc attggctgat ttttaaaggc cagctgtgcg aactcttttt cgtaacgctc
     1561 cagttccatc cccaatgcgc ggttaaatgg catgtgataa acaaacatct cacccactaa
     1621 tttcagggct tgttcagcgg tcagtacggc agacatatca tccttacact tcattggtta
     1681 atgaaatgtt gattttatgc ttctttgttg ttggtttcta ctttaggaag ggataactaa
     1741 cggctatgga gttaagtatg taaaatagcc cgcagaaaaa tattcacctt atcaataatt
     1801 cgttacggag aacacgaccg atgcggactc tgcagggctg gttgttgccg gtgtttatgt
     1861 tgcctatggc agtatatgcg caagaggcaa cggtgaaaga ggtgcatgac gcgccagcgg
     1921 tgcgtggcag tattatcgcc aatatgctgc aggagcatga caatccgttc acgctctatc
     1981 cttatgacac caactacctc atttacaccc aaaccagcga tctgaataaa gaagcgattg
     2041 ccagttacga ctgggcggaa aatgcgcgta aggatgaagt aaagtttcag ttgagcctgg
     2101 catttccgct gtggcgtggg attttaggcc cgaactcggt gttgggtgcg tcttatacgc
     2161 aaaaatcctg gtggcaactg tccaatagcg aagagtcttc accgtttcgt gaaaccaact
     2221 acgaaccgca attgttcctc ggttttgcca ccgattaccg ttttgcaggt tggacgctgc
     2281 gcgatgtgga gatggggtat aaccacgact ctaacgggcg ttccgacccg acctcccgca
     2341 gctggaaccg cctttatact cgcctgatgg cagaaaacgg taactggctg gtagaagtga
     2401 agccgtggta tgtggtgggt aatactgacg ataacccgga tatcaccaaa tatatgggtt
     2461 actaccagct taaaatcggc tatcacctcg gtgatgcggt gctcagtgcg aaaggacagt
     2521 acaactggaa caccggctac ggcggcgcgg agttaggctt aagttacccg atcaccaaac
     2581 atgtgcgcct ttatactcag gtttacagcg gctatggcga atcgctcatc gactataact
     2641 tcaaccagac ccgtgtcggt gtgggggtta tgctaaacga tttgttttga tgaacggttg
     2701 agtggttggc aaatctggaa tccagcatcc aggattaccc tctcagagac taaaagcatt
     2761 gcagtttctc gcgcaggcgc tgaaaatagc gcctgttttt atttcaggca atcggggtga
     2821 atgtggcgca ggcggaagtg ttgaatctgg agtccggagc taaacaggtt ttacaagaaa
     2881 cctttggcta ccaacagttt cgccccggcc aggaagaaat tatcgacact gtgctttccg
     2941 gtcgcgattg cctggtcgtc atgcccaccg gtggcggaaa atccctttgc tatcaaattc
     3001 ctgccttatt gctaaacggc cttaccgtgg ttgtttcacc gctgatttcg ttgatgaaag
     3061 atcaggtgga tcaactgcaa gccaacggcg tggcggcggc gtgccttaac tcgacgcaaa
     3121 cccgcgaaca gcaacttgaa gtgatgacag gctgccgcac cgggcaaatt cgtctgcttt
     3181 atatcgcccc ggaacgcctg atgctggata actttcttga gcatctggcg cactggaatc
     3241 cggtgttatt agccgttgat gaagcgcact gtatctccca atggggccac gatttccgcc
     3301 cggaatatgc cgcgctcggt cagttgcgcc agcggttccc gacgctgccg tttatggcgc
     3361 tgaccgccac agccgacgac accacgcgcc aggatatcgt gcgcctgctg gggctgaacg
     3421 atccgctgat tcaaatcagc agttttgacc gtccgaatat tcgctacatg ctgatggaga
     3481 agttcaaacc gctcgatcag ttgatgcgct acgtgcagga acagcgcggt aagtccggca
     3541 ttatctactg caacagccgg gcgaaagtag aagacaccgc tgcgcgcctg caaagcaagg
     3601 gtattagcgc ggcggcctat catgccgggc tggaaaataa tgtccgcgcc gacgtgcagg
     3661 agaaattcca gcgcgatgac ctgcaaattg tggtggcgac ggtggcgttc ggcatgggca
     3721 tcaataaacc aaacgttcgc ttcgtggtcc actttgatat tccgcgcaat atcgaatcct
     3781 attatcagga aaccggtcgc gccgggcgtg atggcctgcc cgcggaagcg atgctgtttt
     3841 acgatccggc cgatatggcg tggcttcgcc gttgtctgga agagaagccg caggggcagt
     3901 tgcaggatat cgaacgtcat aaactcaatg cgatgggcgc ttttgccgaa gcgcaaactt
     3961 gccgtcgtct ggtactgctg aactattttg gcgaagggcg tcaggagccg tgcgggaact
     4021 gcgatatctg cctcgatccg ccgaaacagt acgacggttc aaccgatgct cagattgccc
     4081 tttccaccat tggtcgtgtg aatcagcgtt ttggtatggg ctatgtggtg gaagtgattc
     4141 gcggcgctaa taaccagcgt atccgcgact atggtcatga caaactaaaa gtctacggta
     4201 tgggccgtga taaaagtcat gaacattggg tgagcgtgat ccgccagctg attcacctcg
     4261 gtctggtgac gcaaaatatt gcccagcatt ctgccctaca actgacagag gctgcgcgtc
     4321 cggtgctgcg cggcgaatcc tctttgcaac ttgccgtgcc gcgtatcgtg gcgctcaaac
     4381 cgaaagcgat gcagaaatcg ttcggcggca actatgatcg caaactgttc gccaaattac
     4441 gcaaactgcg taaatcgata gccgatgaaa gtaatgtccc gccgtacgtg gtgtttaacg
     4501 acgcaacctt gattgagatg gctgaacaga tgccgatcac cgccagcgaa atgctcagcg
     4561 ttaacggcgt tgggatgcgc aagctggaac gctttggcaa accgtttatg gcgctgattc
     4621 gtgcgcatgt tgatggcgat gacgaagagt agtcagcagc ataaaaaagt gccagtatga
     4681 agactccgta aacgtttccc ccgcgagtca aatgtatgtt gatgttattt ctcaccgtcg
     4741 ccatggtgca cattgtggcg cttatgagcc ccggtcccga tttctttttt gtctctcaga
     4801 ccgctgtcag tcgttcccgt aaagaagcga tgatgggcgt gctgggcatt acctgcggcg
     4861 taatggtttg ggctgggatt gcgctgcttg gcctgcattt gattatcgaa aaaatggcct
     4921 ggctgcatac gctgattatg gtgggcggtg gcctgtatct ctgctggatg ggttaccaga
     4981 tgctacgcgg tgcactgaaa aaagaggcgg tttctgcacc tgcgccacag gtcgagctgg
     5041 cgaaaagtgg gcgcagtttc ctgaaaggtt tactgaccaa tctcgctaat ccgaaagcga
     5101 ttatctactt tggctcggtg ttctcattgt ttgtcggtga taacgttggc actaccgcgc
     5161 gctggggcat ttttgcgctg atcattgtcg aaacgctggc gtggtttacc gtcgttgcca
     5221 gcctgtttgc cctgccgcaa atgcgccgtg gttatcaacg tctggcgaag tggattgatg
     5281 gttttgccgg ggcgttattt gccggatttg gcattcattt gattatttcg cggtgatgcc
     5341 agacgcgtct tcagagtaag tcggataagg cgtttacgcc gcatccgaca ttatttttca
     5401 cgcatgcctc gccgatgcta acagcgctcc caccagcata aacaacgagc cgaaaatctt
     5461 attcagcgcc ttcatctgct ttggtccttt aatccataga gcaatccgtt gagcaagggt
     5521 ggcgtaaccg atcatcacaa taatatcgac cacaatagtg gtgacgccga gcacgatata
     5581 ctgcatcagt tgcggctgtt gcggcatgat gaattgcgga aatagcgccg ccagaaacac
     5641 aatacttttg ggattggtga gattcacaaa aactgcgcgc tggaacaaat gtcgacgcga
     5701 ttgagtagag gccagcgatt taaggtcaat tgcaccagcg gcgcgccact gctggattcc
     5761 cagccaaatc aagtaagccg cgcctgccca cttcaacact tcaaacgcaa tcactgagcg
     5821 ggaaaatagc gtccccaacc ccacgccaac caacacaata tgaatcgcca gtccggtctg
     5881 aagcccagca atagacgcca ccgcgccgcg ataaccgtgg ttgagcgagg tggtcatagt
     5941 gttgattgca ccagagcctg gcgacaggct taaaatgatc gatgtcagca ggtaggcaaa
     6001 ccaccattct aaggtcatga tgaactcccg gtgtgtctat ttttgtgcca caatacgcta
     6061 ctgtcgcagc gttgtgtcag gcacgctaaa aaaaacgatt ttacgtggtt taagaggcag
     6121 attacccgat gtttcagcag caaaaagact gggaaacaag agaaaacgcg tttgctgctt
     6181 ttaccatggg accgctgact gatttctggc gtcagcgtga tgaagcagag tttactggtg
     6241 tggatgacat tccggtgcgc tttgtccgtt ttcgcgcaca gcaccatgac cgggtggtag
     6301 tcatctgccc ggggcgtatt gagagctacg taaaatatgc ggaactggcc tatgacctgt
     6361 tccatttggg gtttgatgtc ttaatcatcg accatcgcgg gcagggacgt tccggtcgcc
     6421 tgttagccga tccgcatctc gggcatgtta atcgctttaa tgattatgtt gatgatctgg
     6481 cggcattctg gcagcaggag gttcagcccg gtccgtggcg taaacgctat atactggcac
     6541 attcgatggg cggtgcgatc tccacattat ttctgcaacg ccatccaggt gtatgtgacg
     6601 ccattgcgct aactgcgcca atgtttggga tcgtgattcg tatgccgtca tttatggcac
     6661 ggcagatcct caactgggcc gaagcgcatc cacgtttccg tgatggctat gcaataggca
     6721 ccgggcgctg gcgcgcgttg ccgtttgcta tcaacgtact gacccacagc agacagcgat
     6781 atcgacgtaa cttacgcttc tatgctgatg acccaacgat tcgcgtcggt gggccgacct
     6841 accattgggt acgcgaaagt attctggctg gcgaacaggt gttagccggt gcgggtgatg
     6901 atgccacgcc aacgcttctc ttacaagctg aagaggaacg cgtggtggat aaccgcatgc
     6961 atgaccgttt ttgtgaactc cgcaccgccg cgggccatcc tgtcgaagga ggacggccgt
     7021 tggtaattaa aggtgcttac catgagatcc tttttgaaaa ggacgcaatg cgctcagtcg
     7081 cgctccacgc catcgttgat tttttcaaca ggcataactc acccagcgga aaccgctcta
     7141 cagaggttta aatttcttat gtaccaggtt gttgcgtctg atttagatgg cacgttactt
     7201 tctcccgacc atacgttatc cccttacgcc aaagaaactc tgaagctgct caccgcgcgc
     7261 ggcatcaact ttgtgtttgc gaccggtcgt caccacgttg atgtggggca aattcgcgat
     7321 aatctggaga ttaagtctta catgattacc tccaatggtg cgcgcgttca cgatctggat
     7381 ggtaatctga tttttgctca taacctggat cgcgacattg ccagcgatct gtttggcgta
     7441 gtcaacgaca atccggacat cattactaac gtttatcgcg acgacgaatg gtttatgaat
     7501 cgccatcgcc cggaagagat gcgctttttt aaagaagcgg tgttcaaata tgcgctgtat
     7561 gagcctggat tactggagcc ggaaggcgtc agcaaagtgt ttttcacctg cgattcccat
     7621 gaacaactgc tgccgctgga gcaggcgatt aacgctcgct ggggcgatcg cgtcaacgtc
     7681 agtttctcta ccttaacctg tctggaagtg atggcgggcg gcgtgtcgaa aggccatgcg
     7741 ctggaagcag tggcgaagaa actgggctac agcctgaagg attgtattgc gtttggtgac
     7801 gggatgaacg acgccgaaat gctgtcgatg gcggggaaag gctgcattat gggcagtgcg
     7861 caccagcgtc tgaaagacct tcatcccgag ctggaagtga ttggtactaa tgccgacgac
     7921 gcggtgccgc attatctgcg taaactctat ttatcgtaat cgttctttat ttggtcagtt
     7981 gtcaacctga tacttcgcta caatggatac tcgttaatca aagagttttc cattgtggcg
     8041 ctacttatca tcaccacgat tctgtgggcc ttctccttta gcttttatgg cgagtacctt
     8101 gcggggcacg tcgatagcta ttttgcggtg ctggtgcgcg ttggcctggc ggcactcgtt
     8161 tttctgccgt ttctgcgtac ccgtggcaat agcctgaaaa cggtcggcct gtatatgttg
     8221 gtgggcgcga tgcagctcgg cgtgatgtat atgctgagtt tccgcgcgta tctctacctg
     8281 acggtctccg aactgctgct gttcaccgta ctgacgccgc tctacatcac gctgatttat
     8341 gacatcatga gtaagcgccg tctgcgctgg ggctatgctt ttagcgcctt gctggcggtg
     8401 attggtgccg ggattattcg ctatgatcag gtcaccgacc atttctggac tggcttgttg
     8461 ctggtgcaac tctccaatat cacttttgcc atcggcatgg tgggttacaa acgcctgatg
     8521 gaaactcgcc cgatgccgca gcataacgcc tttgcgtggt tctatcttgg cgcgttcctg
     8581 gtggcggtaa ttgcatggtt cttgctggga aatgcgcaga aaatgccgca aaccacgttg
     8641 caatggggca ttctggtgtt tcttggcgtg gtggcttccg ggattggcta ctttatgtgg
     8701 aactacggcg cgacgcaggt ggacgccgga acgctgggca ttatgaataa tatgcacgtt
     8761 ccggcagggc tgctggttaa cctggctatc tggcaccaac agctgcactg gccaacgttt
     8821 attacaggcg cgctggtgat cctggcctca ctgtgggtgc atcgtaagtg ggtcgctccg
     8881 cgctcttcac aaacggcaga tgatcgcagg cgtgattgcg cgctgagcga ataaacgctt
     8941 ccgtaactgg ctgacgctgc tcgccatcgc gcacggcggc gtacagtcgg ctccacaagc
     9001 cttcgcccag ggttttggtc accaccagac cctggcgctc aaaactctct actacccaat
     9061 gcggtagcgc ggcaataccc atccgcgcgg caaccatctg aatcaacaat aaggtgttat
     9121 cgacgctttt cagtgacggg ctgacgcctg ccggctgaag aaaatgccgc cagacatcca
     9181 gtcgactacg ctgcaccgga taaattaata gcgtctcgct ggcgagatct tccggtgtaa
     9241 tacgcgtttt tgccgccagt ggatggtcag gtgctaacac caggcgtact tcataatcga
     9301 acatcggcga ataatgcagg ccactgcgcg gcaggatatc ggaggtcatc accagatcca
     9361 gctcgccctg ttgcaacgcg ggctgcgggt caaacgtcac gcccgattta aaatccatct
     9421 ctacctgcgg ccagttctta tggaaatttt ctaacgcggg tgtcagccac tgaatacagc
     9481 tatggcactc aatggcaatg cgcagacgcg tctgctgcgg ttcattgcag gcttgtagtg
     9541 cctggctgat ttgcggcagc acctggtttg ccagttgcaa caggatttct ccctgcggtg
     9601 taaagcgtag cggctggctc ttacgcacaa atagccggaa accaaggcgt tgttccagat
     9661 cgctaaactg gtgagacagg gcggattgcg tctgatgcaa cgtcgccgca gcggctgcga
     9721 gcgagccgca gttccgcaac gcttgtagcg ttttcaggtg ttttacttcg atcatgaaag
     9781 tccttcactt cggcatgaat aatttgcgct tgaggaatat acagtaaccg ccaattatgg
     9841 atgtgtaaac atctggacgg ctaaatacta aaaattccat aaggggcata taatgacaat
     9901 tcttaatcac accctcggtt tccctcgcgt tggcctgcgt cgcgagctga aaaaagcgca
     9961 agaaagttat tgggcgggga actccacgcg tgaagaactg ctgacagtag ggcgtgaact
    10021 gcgtgctcgt cactgggatc aacaaaagca agcgggtatc gacctgctgc cggtgggcga
    10081 ttttgcctgg tacgatcatg tactgaccac cagtttgctg ctgggtaatg ttccgccacg
    10141 tcatcagaac aaagacggtt cggtagatat cgacaccctg ttccgtattg gtcgtggacg
    10201 tgcgccgact ggcgaacctg cggcggcagc ggaaatgacc aaatggttta acaccaacta
    10261 tcactacatg gtgccggagt tcgttaaagg ccaacagttc aaactgacct ggacgcagct
    10321 gctggaggaa gtggacgagg cgctggcgct gggccacaac gttaagcctg tgctgctggg
    10381 gccggttact tacctgtggc tggggaaagt gaaaggtgaa cagtttgacc gccttagcct
    10441 gctgaacgac attctgccgg tttatcagca agtgctggca gaactggcga aacgcggcat
    10501 cgagtgggta cagattgatg aacccgcgct ggtactggaa ctaccacagg cgtggctgga
    10561 cgcatacaaa cccgcttacg acgcgctcca gggacaggtg aaactgctgc tgaccaccta
    10621 ttttgaaggc gtaacgccaa atctcgacac gattactgcg ctgcctgttc agggtctgca
    10681 tgttgacctc gtacatggta aagatgacgt tgctgaactg cacaagcgcc tgccttctga
    10741 ctggttgctg tctgcgggtc tgatcaatgg tcgtaacgtc tggcgcgccg atcttaccga
    10801 gaaatatgcg caaattaagg acattgtcgg caaacgtgat ttgtgggtgg catcttcctg
    10861 ctcgttgctg cacagcccca tcgacctgag cgtggaaacg cgtcttgatg cagaagtgaa
    10921 aagctggttt gccttcgccc tacaaaaatg ccatgaactg gcactgctgc gcgatgcgct
    10981 gaacagtggt gacacggcag ctctggcaga gtggagcgcc ccaattcagg cgcgtcgtca
    11041 ctctactcgt gtacataatc cggcggtaga aaagcgtctg gcggcgatca ccgcccagga
    11101 cagccagcgt gcgaatgtct atgaagtgcg tgctgaagcc cagcgtgcgc gttttaaact
    11161 gccagcgtgg ccgaccacca cgattggttc cttcccgcaa accacggaaa ttcgtaccct
    11221 gcgtctggat ttcaaaaagg gcaatctcga cgccaacaac taccgcacgg gcattgcgga
    11281 acatatcaag caggccattg ttgagcagga acgtttggga ctggatgtgc tggtacatgg
    11341 cgaggccgag cgtaatgaca tggtggaata ctttggcgag cacctcgacg gatttgtctt
    11401 tacgcaaaac ggttgggtac agagctacgg ttcccgctgc gtgaagccac cgattgttat
    11461 tggtgacgtg agccgaccgg caccgattac cgtggagtgg gcgaaatatg cgcagtcgct
    11521 gaccgacaaa ccggtgaaag ggatgctgac tggtccggtg acgattctct gctggtcgtt
    11581 cccgcgtgaa gatgtgagcc gtgaaaccat cgccaaacag attgcgctgg cgctgcgtga
    11641 tgaagtggcg gacctggaag ccgctggaat tggcatcatc cagattgatg aaccggcgct
    11701 gcgcgaaggt ttaccgctgc gtcgtagcga ctgggacgcc tatctccagt ggggcgtaga
    11761 ggccttccgt atcaacgccg ccgtggcgaa agatgacaca caaatccaca ctcacatgtg
    11821 ttattgcgag ttcaacgaca tcatggattc cattgcggcg ctggatgcgg atgtgattac
    11881 catcgaaact tcgcgttctg acatggagtt gctggagtcg ttcgaagagt ttgattatcc
    11941 caatgaaatc ggtccaggcg tctatgatat tcactcgcca aacgtaccga gcgtggaatg
    12001 gattgaagcc ctgctgaaga aagcggcaaa acgcattccg gcagagcgcc tgtgggttaa
    12061 cccggactgt ggcctgaaaa cgcgcggctg gccagaaacc cgcgcggcac tggcgaacat
    12121 ggtgcaggcg gcgcagaact tgcgtcgggg gtaaatccaa accgggtggt aataccaccc
    12181 ggtcttttct cattacagcg acttcttccc accatactgc ttaaaccatt ccagcatacg
    12241 ctgccagcca tctttcgcag attcggcatg atagctcggg cgataatcag cgttgaatgc
    12301 atgcccggca tccgggtaca cgataatctc tgctttcgca ttagcagccc gcagcgcctg
    12361 gcgcatggtt tcaacgctct cctgcggaat gctgttatcc tgaccaccat ataagccgag
    12421 aaccggcgcg ttaagatcgg ttgcgatatc aacaggttgt ttcggtgaat tcagcgactt
    12481 gtcgcccgtc agtttgccgt accacgccac tgcggctttt agctgtggat tatgcgcggc
    12541 atacagccag gtgatacgtc caccccagca gaatccggtg atcattaaac gatgaacatc
    12601 gccgccgttg cgggaagccc aactggcgac atgatcgaga tcggccagca cctgcgagtc
    12661 aggcactttt gctaccagac cgctaagcaa cgtggggata tcggcaaaat cattcggatc
    12721 gccttcgcgg aagtaaagtt caggtgcgat agccagatac ccctccagcg ccagacggcg
    12781 acaaatgtcg cggatatgtt catgcacgcc aaaaatttcc tgcactacaa tgaccactgg
    12841 cagtgggcca tcgctttgct ttggtctggc atggtaagca ggcatgttat ccccttgtga
    12901 agggatagag gtgaagcccg ccacaatcgc gtcgtccggg gtctgaacga tggtcgaagc
    12961 gagaggcgat gcagcaggtg caaatccaga ttgttgtgtt gttgccatgg tattctccgt
    13021 acccttataa aaatgttgcg caatgttaac tatagtcagc atgcaacaaa tcacattgcc
    13081 tgaatcggct catcttttat gcagtcctgc agaatgaaga gtgatttatg tgatttacat
    13141 cacttttgat gggtaaattt atgcaacgcg tttgcgtcat ggtgatgagt atcacgaaaa
    13201 aatgttaaac ccttcggtaa agtgtctttt tgcttcttct gactaaaccg attcacagag
    13261 gagttgtata tgtccaagtc tgatgttttt catctcggcc tcactaaaaa cgatttacaa
    13321 ggggctacgc ttgccatcgt ccctggcgac ccggatcgtg tggaaaagat cgccgcgctg
    13381 atggataagc cggttaagct ggcatctcac cgcgaattca ctacctggcg tgcagagctg
    13441 gatggtaaac ctgttatcgt ctgttctacc ggtatcggcg gcccgtctac ctctattgct
    13501 gttgaagagc tggcacagct gggcattcgc accttcctgc gtatcggtac aacgggcgct
    13561 attcagccgc atattaatgt gggtgatgtc ctggttacca cggcgtctgt ccgtctggat
    13621 ggcgcgagcc tgcacttcgc accgctggaa ttcccggctg tcgctgattt cgaatgtacg
    13681 actgcgctgg ttgaagctgc gaaatccatt ggcgcgacaa ctcacgttgg cgtgacagct
    13741 tcttctgata ccttctaccc aggtcaggaa cgttacgata cttactctgg tcgcgtagtt
    13801 cgtcacttta aaggttctat ggaagagtgg caggcgatgg gcgtaatgaa ctatgaaatg
    13861 gaatctgcaa ccctgctgac catgtgtgca agtcagggcc tgcgtgccgg tatggtagcg
    13921 ggtgttatcg ttaaccgcac ccagcaagag atcccgaatg ctgagacgat gaaacaaacc
    13981 gaaagccatg cggtgaaaat cgtggtggaa gcggcgcgtc gtctgctgta attctcttct
    14041 cctgtctgaa ggccgacgcg ttcggccttt tgtatttttg cgtagcgcct cgcaggaaat
    14101 gcctttccaa ctggacgttt gtacagcaca attctatttt gtgcgggtaa gttgttgcgt
    14161 caggaggcgt tgtggatttc tcaatcatgg tttacgcagt tattgcgttg gtgggtgtgg
    14221 caattggctg gctgtttgcc agttatcaac atgcgcagca aaaagccgag caattagctg
    14281 aacgtgaaga gatggtcgcg gagttaagcg cggcaaaaca acaaattacc caaagcgagc
    14341 actggcgtgc agagtgcgag ttactcaata acgaagtgcg cagcctgcaa agtattaaca
    14401 cctctctgga ggccgatctg cgtgaagtaa ccacgcggat ggaagccgca cagcaacatg
    14461 ctgacgataa aattcgccag atgattaaca gcgagcagcg cctcagtgag cagtttgaaa
    14521 acctcgccaa ccgtattttt gagcacagca atcgccgggt tgatgagcaa aaccgtcaga
    14581 gtctgaacag cctgttgtcg ccgctacgtg aacaactgga cggttttcgc cgtcaggttc
    14641 aggacagctt cggtaaagaa gcacaagaac gccataccct gacccacgaa attcgcaatc
    14701 tccagcaact caacgcgcaa atggcccagg aagcgatcaa cctgacgcgc gcgctgaaag
    14761 gcgacaataa aacccagggc aactggggcg aggtagtatt gacgcgggtg ctggaggctt
    14821 ccggtctgcg tgaagggtat gaatatgaaa cccaggtcag catcgaaaat gacgcccgct
    14881 cgcggatgca accggatgtc atcgtgcggc tgccgcaggg aaaagatgtg gtgatcgacg
    14941 ccaaaatgac gctggtcgcc tatgaacgct atttcaacgc cgaagatgac tacacccgcg
    15001 aaagcgcgct acaggaacat atcgcgtcgg tgcgtaacca tatccgtttg ctgggacgca
    15061 aagattatca acagctgccg gggctgcgaa ctctggatta cgtgctgatg tttattcccg
    15121 ttgaacccgc ttttttactg gcgcttgacc gccagccgga gctgatcacc gaagcgttga
    15181 aaaacaatat catgctggtt agcccgacta cgctgctggt ggcgttgcgt actattgcca
    15241 acctgtggcg ttatgagcat caaagccgca acgcccagca aatcgccgat cgtgccagca
    15301 agctgtacga caagatgcgt ttgttcatcg atgacatgtc cgcgattggt caaagtctcg
    15361 acaaagcgca ggataactac cgccaggcaa tgaaaaaact ctcttcaggt cgcggaaatg
    15421 tgctggcgca ggcagaagcg tttcgcggtt taggagtaga aattaaacgc gagattaatc
    15481 cggatttggc tgaacaggcg gtgagccagg atgaagagta tcgacttcgg tcggttccgg
    15541 agcagccgaa tgatgaagct tatcaacgcg atgatgaata taatcagcag tcgcgctagc
    15601 ccattagggg tagttaagcc gggtagaaat ctagggcatc gacgcccaat ctgttacact
    15661 tctggaacaa ttttttgatg agcaggcatt gagatggtgg ataagtcaca agaaacgacg
    15721 cactttggtt ttcagaccgt cgcgaaggaa caaaaagcgg atatggtcgc ccacgttttc
    15781 cattccgtgg catcaaaata cgatgtcatg aatgatttga tgtcatttgg tattcatcgt
    15841 ttgtggaagc gattcacgat tgattgcagc ggcgtacgcc gtgggcagac cgtgctggat
    15901 ctggctggtg gcaccggcga cctgacagcg aaattctccc gcctggtcgg agaaactggc
    15961 aaagtggtcc ttgctgatat caatgaatcc atgctcaaaa tgggacgcga gaagctgcgt
    16021 aatatcggtg tgattggcaa cgttgagtat gttcaggcga acgctgaggc gctgccgttc
    16081 ccggataaca cctttgattg catcaccatt tcgtttggtc tgcgtaacgt caccgacaaa
    16141 gataaagcac tgcgttcaat gtatcgcgtg ctgaaacccg gcggccgcct gctggtgctt
    16201 gagttctcga agccaattat cgagccgctg agcaaagcct atgatgcata ctccttccat
    16261 gtgctgccgc gtattggctc actggtcgcg aacgacgccg acagctaccg ttatctggca
    16321 gaatccatcc gtatgcatcc cgatcaggat accctgaaag ccatgatgca ggatgccgga
    16381 ttcgaaagtg tcgactacta caatctgacg gcaggggttg tggcgctgca tcgtggttat
    16441 aagttctgac aggagaccgg aaatgccttt taaaccttta gtgacggcag gaattgaaag
    16501 tctgctcaac accttcctgt atcgctcacc cgcgctgaaa acagcccgct cgcgtctgct
    16561 gggtaaagta ttgcgcgtgg aggtaaaagg cttttcgacg tcattgattc tggtgttcag
    16621 cgaacgccag gttgatgtac tgggcgaatg ggcaggcgat gctgactgca ccgttatcgc
    16681 ctacgccagt gtgttgccga aacttcgcga tcgccagcag cttaccgcac tgattcgcag
    16741 tggtgagctg gaagtgcagg gcgatattca ggtggtgcaa aacttcgttg cgctggcaga
    16801 tctggcagag ttcgaccctg cggaactgct ggccccttat accggtgata tcgccgctga
    16861 aggaatcagc aaagccatgc gcggaggcgc aaagttcctg catcacggca ttaagcgcca
    16921 gcaacgttat gtggcggaag ccattactga agagtggcgt atggcacccg gtccgcttga
    16981 agtggcctgg tttgcggaag agacggctgc cgtcgagcgt gctgttgatg ccctgaccaa
    17041 acggctggaa aaactggagg ctaaatgacg ccaggtgaag tacggcgcct atatttcatc
    17101 attcgcactt ttttaagcta cggacttgat gaactgatcc ccaaaatgcg tatcaccctg
    17161 ccgctacggc tatggcgata ctcattattc tggatgccaa atcggcataa agacaaactt
    17221 ttaggtgagc gactacgact ggccctgcaa gaactggggc cggtttggat caagttcggg
    17281 caaatgttat caacccgccg cgatcttttt ccaccgcata ttgccgatca gctggcgtta
    17341 ttgcaggaca aagttgctcc gtttgatggc aagctggcga agcagcagat tgaagctgca
    17401 atgggcggct tgccggtaga agcgtggttt gacgattttg aaatcaagcc gctggcttct
    17461 gcttctatcg cccaggttca taccgcgcga ttgaaatcga atggtaaaga ggtggtgatt
    17521 aaagtcatcc gcccggatat tttgccggtc attaaagcgg atctgaaact tatctaccgt
    17581 ctggctcgct gggtgccgcg tttgctgccg gatggtcgcc gtctgcgccc aaccgaagtg
    17641 gtgcgcgagt acgaaaagac cttgattgat gaactgaatt tgctgcggga atctgccaac
    17701 gccattcagc ttcggcgcaa ttttgaagac agcccgatgc tctacatccc ggaagtttac
    17761 cctgactatt gtagtgaagg gatgatggtg atggagcgca tttacggcat tccggtgtct
    17821 gatgttgcgg cgctggagaa aaacggcact aacatgaaat tgctggcgga acgcggcgtg
    17881 caggtgttct tcactcaggt ctttcgcgac agctttttcc atgccgatat gcaccctggc
    17941 aacatcttcg taagctatga acacccggaa aacccgaaat atatcggcat tgattgcggg
    18001 attgttggct cgctaaacaa agaagataaa cgctatctgg cagaaaactt tatcgccttc
    18061 tttaatcgcg actatcgcaa agtggcagag ctacacgtcg attctggctg ggtgccacca
    18121 gataccaacg ttgaagagtt cgaatttgcc attcgtacgg tctgtgaacc tatctttgag
    18181 aaaccgctgg ccgaaatttc gtttggacat gtactgttaa atctgtttaa tacggcgcgt
    18241 cgcttcaata tggaagtgca gccgcaactg gtgttactcc agaaaaccct gctctatgtc
    18301 gaaggggtag gacgccagct ttatccgcag ctcgatttat ggaaaacagc gaagcctttc
    18361 ctggagtcgt ggattaaaga tcaggtcggt attcctgcgc tggtgagagc atttaaagaa
    18421 aaagcgccgt tctgggtcga aaaaatgcca gaactgcctg aattggttta cgacagtttg
    18481 cgccagggca agtatttaca gcacagtgtt gataagattg cccgcgagct tcagtcaaat
    18541 catgtacgtc agggacaatc gcgttatttt ctcggaattg gcgctacgtt agtattaagt
    18601 ggcacattct tgttggtcag ccgacctgag tgggggctga tgcccggctg gttaatggca
    18661 ggtggtctga tcgcctggtt tgtcggttgg cgcaaaacac gctgattttt tcatcgctca
    18721 aggcgggccg tgtaacgtat aatgcggctt tgtttaatca tcatctacca cagaggaaca
    18781 tgtatgggtg gtatcagtat ttggcagtta ttgattattg ccgtcatcgt tgtactgctt
    18841 tttggcacca aaaagctcgg ctccatcggt tccgatcttg gtgcgtcgat caaaggcttt
    18901 aaaaaagcaa tgagcgatga tgaaccaaag caggataaaa ccagtcagga tgctgatttt
    18961 actgcgaaaa ctatcgccga taagcaggcg gatacgaatc aggaacaggc taaaacagaa
    19021 gacgcgaagc gccacgataa agagcaggtg taatccgtgt ttgatatcgg ttttagcgaa
    19081 ctgctattgg tgttcatcat cggcctcgtc gttctggggc cgcaacgact gcctgtggcg
    19141 gtaaaaacgg tagcgggctg gattcgcgcg ttgcgttcac tggcgacaac ggtgcagaac
    19201 gaactgaccc aggagttaaa actccaggag tttcaggaca gtctgaaaaa ggttgaaaag
    19261 gcgagcctca ctaacctgac gcccgaactg aaagcgtcga tggatgaact acgccaggcc
    19321 gcggagtcga tgaagcgttc ctacgttgca aacgatcctg aaaaggcgag cgatgaagcg
    19381 cacaccatcc ataacccggt ggtgaaagat aatgaagctg cgcatgaggg cgtaacgcct
    19441 gccgctgcac aaacgcaggc cagttcgccg gaacagaagc cagaaaccac gccagagccg
    19501 gtggtaaaac ctgctgcgga cgctgaaccg aaaaccgctg caccttcccc ttcgtcgagt
    19561 gataaaccgt aaacatgtct gtagaagata ctcaaccgct tatcacgcat ctgattgagc
    19621 tgcgtaagcg tctgctgaac tgcattatcg cggtgatcgt gatattcctg tgtctggtct
    19681 atttcgccaa tgatatctat cacctggtat ccgcgccatt gatcaagcag ttgccgcaag
    19741 gttcaacgat gatcgccacc gacgtggcct cgccgttctt tacgccgatc aagctgacct
    19801 ttatggtgtc gctgattctg tcagcgccgg tgattctcta tcaggtgtgg gcatttatcg
    19861 ccccagcgct gtataagcat gaacgtcgcc tggtggtgcc gctgctggtt tccagctctc
    19921 tgctgtttta tatcggtatg gcattcgcct actttgtggt ctttccgctg gcatttggct
    19981 tccttgccaa taccgcgccg gaaggggtgc aggtatccac cgacatcgcc agctatttaa
    20041 gcttcgttat ggcgctgttt atggcgtttg gtgtctcctt tgaagtgccg gtggcaattg
    20101 tgctgctgtg ctggatgggg attacctcgc cagaagactt acgcaaaaaa cgcccgtatg
    20161 tgctggttgg tgcattcgtt gtcgggatgt tgctgacgcc gccggatgtc ttctcgcaaa
    20221 cgctattggc gatcccgatg tactgtctgt ttgaaatcgg tgtcttcttc tcacgctttt
    20281 acgttggtaa agggcgaaat cgggaagagg aaaacgacgc tgaagcagaa agcgaaaaaa
    20341 ctgaagaata aattcaaccg cccgtcaggg cggttgtcat atggagtaca ggatgtttga
    20401 tatcggcgtt aatttgacca gttcgcaatt tgcgaaagac cgtgatgatg ttgtagcgcg
    20461 cgcttttgac gcgggagtta atgggctact catcaccggc actaacctgc gtgaaagcca
    20521 gcaggcgcaa aagctggcgc gtcagtattc gtcctgttgg tcaacggcgg gcgtacatcc
    20581 tcacgacagc agccagtggc aagctgcgac tgaagaagcg attattgagc tggccgcgca
    20641 gccagaagtg gtggcgattg gtgaatgtgg tctcgacttt aaccgcaact tttcgacgcc
    20701 ggaagagcag gaacgcgctt ttgttgctca gctacgcatt gccgcagaat taaacatgcc
    20761 ggtatttatg cactgtcgcg atgcccacga gcggtttatg acattgctgg agccgtggct
    20821 ggataaactg cctggtgcgg ttcttcattg ctttaccggc acacgcgaag agatgcaggc
    20881 gtgcgtggcg cgtggaattt atatcggcat taccggttgg gtttgcgatg aacgacgcgg
    20941 gctggagctg cgggaattgt tgccgttgat tccggcggag aaattgctga tcgaaactga
    21001 tgcgccgtat ctgctcccgc gcgatctcac gccaaagcca tcatcccggc gcaacgagcc
    21061 agcccatctg ccccatattt tgcaacgtat tgcgcactgg cgtggagaag atgccgcatg
    21121 gctggctgcc accacggatg ccaatgtcaa aacactgttt gggattgcgt tttagagttt
    21181 gcggaactcg gtgttcttca cactgtgctt aatctcttta ttaataagat taagcaatag
    21241 catggagcga gcctcaccat cgggttcggt gaaaatggcc tgaaagcctt cgaacgcgcc
    21301 ttcggtaata atcaccttat cacccggata aggggttgcc ggatcgacaa tgtctttcgg
    21361 tttatatacc gatagctgat gaatcaccgc cgatgggact atcgctggcg acgcgccaaa
    21421 gcgcacgaag tggctgacac cgcgggtcgc gttgatagtc gtggtatgaa tcacttctgg
    21481 atcaaattcg acaaacaggt agttgggaaa caatggctca ctgactgcag tacgttttcc
    21541 acgcacgatt ttttccaggg tgatcatcgg tgccaggcaa ttcacagcct gtctttcgag
    21601 gtgttcctgg gcacgttgaa gttgcccgcg cttgcagtac agtaaatacc aggattgcat
    21661 aatgactctt atccgtttaa tcggggcgca aggatagcaa aagctttacg ctaagttaat
    21721 tatattcccc ggtttgcgtt ataccgtcag agttcacgct aatttaacaa atttacagca
    21781 tcgcaaagat gaacgccgta taatgggcgt agattaagag gctacaatgg acgccatgaa
    21841 atataacgat ttacgcgact tcctgacact gcttgaacag cagggtgagc taaaacgtat
    21901 cacgctcccg gtggatccgc atctggaaat cactgaaatt gctgaccgca ctttgcgtgc
    21961 cggtgggcct gcgctgttgt tcgaaaaccc taaaggctac tcaatgccgg tgctgtgcaa
    22021 cctgttcggt acgccaaagc gcgtggcaat gggcatgggg caggaagatg tttcggcgct
    22081 acgtgaagtt ggtaaattat tggcgtttct gaaagagccg gagccgccaa aaggtttccg
    22141 cgacctgttt gataaactgc cgcagtttaa gcaagtattg aacatgccga caaagcggct
    22201 gcgtggtgcg ccctgccaac aaaaaatcgt ctctggcgat gacgtcgatc tcaatcgcat
    22261 tcccattatg acctgctggc cggaagatgc cgcgccgctg attacctggg ggctgacagt
    22321 gacgcgcggc ccgcataaag agcggcagaa tctgggcatt tatcgccagc agctgattgg
    22381 taaaaacaaa ctgattatgc gctggctgtc gcatcgcggc ggcgcgctgg attatcagga
    22441 gtggtgtgcg gcgcatccgg gcgaacgttt cccggtttct gtggcgctgg gtgccgatcc
    22501 cgccacgatt ctcggtgcag tcacccccgt tccggataca ctttcagagt atgcgtttgc
    22561 cggattgctg cgcggtacca agaccgaagt ggtgaagtgt atctccaatg accttgaagt
    22621 gcccgccagt gcggagattg tgctggaagg gtatatcgaa caaggtgaaa cggcgccgga
    22681 agggccgtat ggcgaccaca ccggttacta taacgaagtc gatagtttcc cggtatttac
    22741 cgtgacgcat attacccagc gtgaagatgc gatttaccac tccacctata ctggtcgtcc
    22801 gccggatgaa cccgcggtgc tgggtgtcgc actgaacgaa gtgtttgtgc cgattctgca
    22861 aaaacagttc ccggaaattg tcgattttta cctgccgccg gaaggctgtt cttatcgtct
    22921 ggcggtagtg acgatcaaaa aacagtacgc cggacacgcg aagcgcgtca tgatgggcgt
    22981 ctggtcgttc ttacgccagt ttatgtacac taaatttgtg atcgtttgcg atgatgacgt
    23041 caacgcacgc gactggaacg atgtgatttg ggcgattacc acccgtatgg acccggcgcg
    23101 ggatactgtt ctggtagaaa atacgcctat tgattatctg gattttgcct cgcctgtctc
    23161 cgggctgggt tcaaaaatgg ggctggatgc cacgaataaa tggccggggg aaacccagcg
    23221 tgaatgggga cgtcccatca aaaaagatcc agatgttgtc gcgcatattg acgccatctg
    23281 ggatgaactg gctattttta acaacggtaa aagcgcctga tgcgcgtttg ttttgcccta
    23341 tttatcgatc cgacagagaa agcgcatgac aaccttaagc tgtaaagtga cctcggtaga
    23401 agctatcacg gataccgtat atcgtgtccg catcgtgcca gatgcggcct tttcttttcg
    23461 cgctggtcag tatttgatgg tagtgatgga tgagcgcgac aaacgtccgt tctcaatggc
    23521 ttcgacgccg gatgaaaaag ggtttatcga gctgcatatt ggcgcttctg aaatcaacct
    23581 ttacgcgaaa gcagtcatgg accgcatcct caaagatcat caaatcgtgg tcgatattcc
    23641 ccacggagaa gcgtggctgc gcgatgatga agagcgtccg atgattttga ttgcgggcgg
    23701 caccgggttc tcttatgccc gctcgatttt gctgacagcg ttggcgcgta acccaaaccg
    23761 tgatatcacc atttactggg gcgggcgtga agagcagcat ctgtatgatc tctgcgagct
    23821 tgaggcgctt tcgttgaagc atcctggtct gcaagtggtg ccggtggttg aacaaccgga
    23881 agcgggctgg cgtgggcgta ctggcaccgt gttaacggcg gtattgcagg atcacggtac
    23941 gctggcagag catgatatct atattgccgg acgttttgag atggcgaaaa ttgcccgcga
    24001 tctgttttgc agtgagcgta atgcgcggga agatcgcctg tttggcgatg cgtttgcatt
    24061 tatctgagat ataaaaaaac ccgcccctga caggcgggaa gaacggcaac taaaacttta
    24121 ttcagtggca tttagatcta tgtcgcatcc ggcattcggc acacgatgcc tgatgcgacg
    24181 ctggcgcgtc ttatcaggcc tacattggtg ccggatcggt agaccggata aggcgttcac
    24241 gccgcatccg gcaagtggtt aaacccgctc aaacaccgtc gcaataccct gacccagacc
    24301 gatacacatc gtcgccagac caaactggac gtctttgcgc tccatcagat ttagcagcgt
    24361 ggtgctgata cgcgcaccgg aacagcccag cggatgaccc agcgcgatcg cgccaccgtt
    24421 gaggttgatc ttctcgtcaa tctgctccat tagccccaga tctttaatac atggcaggat
    24481 ctgcgcggca aaggcttcat tcatttcaaa cacgccgata tcactgacag aaagccccgc
    24541 ttttttcagc gccagcttcg aggccggaac cgggcagtaa cccataatcg atgggtcaca
    24601 accaacgacc gccatcgaac gcacgcgggc gcgcggctta agacctaatt catgggcgcg
    24661 gctttcactc atcaccagca tggcagctgc gccatcggaa agcgcagaag atgtacccgc
    24721 cgttaccgta ccgcttactg gatcaaacgc cggacgcagc gtggcgaggg cttccacggt
    24781 ggtttccggg cgaatcactt cgtcgtaatt aaactgcttc aggacgccgt cggcatcgtg
    24841 accaccggtc gggataattt catttttaaa tgcacccgac tgcgtggcgg cccaggcgcg
    24901 ggcgtgtgac cgcgcggcaa aggcatcctg catttcacgg ctgataccgt gcatacgcgc
    24961 cagcatttct gccgttaagc ccatcatgcc cgccgctttg gcgacattgc ggctcaggcc
    25021 ggggtgaaaa tcgacgccgt ggctcattgg cacatggccc atatgctcca cgccgccgac
    25081 aagacatgcc tgcgcatcgc cagtcatgat cattcgtgct gcgtcatgca atgcctgcat
    25141 ggatgaacca cacaagcgat tgacggtcac cgccgggaca gagtgtggta cttctgccag
    25201 cagcgccgcg ttacgggcga tattaaaacc ctgctccagc gtctgctgca cacaacccca
    25261 gtaaatatcg tcgagggccg ccgcttccag cgccgggtta cgcgccagca ggctacgcat
    25321 taaatgagcg gagagatctt ctgcacgcac gttacgaaaa gcaccgccct tcgaacggcc
    25381 catcggggtg cgaattgcat cgacaatgac aacctgttcc attgtgactc cttaagccgt
    25441 tttcaggtcg ccaaccggac gggctggctc aaccggggga tagtacggtt cgttatgacg
    25501 cgctttatta cgcagacctt ccggcacttc atacagcggg ccgaggtgct gatattgctg
    25561 tgccatatcg aggtattttg cgctaccgag ggtgtccagc cagcggaacg cgccgccgtg
    25621 gaacggaggg aagcccaggc cgtagaccag cgccatatcc gcttctgccg gagtggcgat
    25681 aatgctttct tccagacagc gcaccacttc gttgaccatc gggatcatca tgcgggcgat
    25741 aatctcttct tcgctgaaat cgcgcttcgg ctggctcact tctgccagca ggtcgtcaac
    25801 gacggcgtct tcttctttct tcggcttacc tttgctgcct tctttataac gccagaaacc
    25861 gaggccgttc ttctgaccaa agcggttggc atcaaacagc gcgtcgatgg tatcgcgata
    25921 atctttctgc atccgctgcg ggaagcctgc tgccatgaca gcctgggcat gatgcgcggt
    25981 gtcaatgccc acgacatcca gcagatatgc cggacccatc ggccagccaa actgtttttc
    26041 cattactttg tcgatcttgc ggaaatccgc gccgtcgcgc agcaacaggc tgaaaccggc
    26101 gaaatacggg aacagcacgc ggttgacgaa gaagccgggg cagtcgttaa ccacaatcgg
    26161 cgttttgccc atcttgctcg cccaggcgac gactttcgcg atggtttcgt cggagctttt
    26221 ctcgccgcga attatttcta ccaacggcat tcggtggacc gggttaaaga agtgcatccc
    26281 gcagaagttt tctggacgtt ccagcgcgtt ggccagttcg ctgataggaa tggttgaagt
    26341 gttagacgcc agcacggtat tcgggcgtac tttctgctcg gtttctgcca gtacggcttt
    26401 tttcactttt gggttttcga caaccgcttc taccacaaca tccacgcggt caaacccggc
    26461 gtagtcgagc gttgggtgga ttgtggagat cacgccagcc agtttcagac catcgatctt
    26521 gccgcgctca agctgcttgt tcagcagttt cgcggcttcg gtcatgccga gggttaacga
    26581 cttgtcgttg atatctttca tgacaaccgg cacgcctttc cacgcagact ggtaagcgat
    26641 gccgccgccc ataatgcctg cacccagcac cgcggcctgt ttcggggttt caacgtcttt
    26701 ggtgagtttc ttcgctttgc cttttacata ttgatcgtta aggaaaatgc cgaccagtgc
    26761 gcgggcttcg ttggtatgcg ccagcgggac aaaacttttg ttttccaggt ttaaggcttc
    26821 ttcacgacca aaacgggccg cagcttcaat ggtttttact gcggtgatgg gggccggata
    26881 atgtttcccc gctgtttgtg cgaccatccc tttagcgatg gtgaagctca tggtggcttc
    26941 aatcttgctc agttttagtg gttccagctt cggctgacgt tttgctttcc agtcgaggtc
    27001 gccgttaatg gcctggcgta aaaccgcctt tgcgccttca accagttttt ctgctttgac
    27061 tacgccatcc accagaccga ttttcagcgc ctgatccgcg ccgacatctt taccggcggc
    27121 aatgatttcc agcgcactgt cagcgcccag catacgtggc atacgtacag aaccgccaaa
    27181 gcccggcatg atgcccagtt tggtttccgg cagaccgatg cgcagatccg gcgtcgccag
    27241 acgataatcg gtcgccagca cgcattcgca gccaccgccc agcgcatagc cgttgacggc
    27301 agcaatggtc ggcaccggca gatcttcctg gcgattaaac acgctattgg caaagtgcag
    27361 ccactgactt aactgttctt caggaacgag gaacagggac aaaaattcgg tgatatcagc
    27421 accgacgata aaggctgctt tgttcgaacg cagcagcagc ccttttagat ctgattgctg
    27481 ttccagcacg ccgatggcct cgccgaggct ggcgacggtc gcagtgtcga gtttattaac
    27541 tgaacctggg gcatcaaata ccagttcggc aatgccatct tccagccagt caaggtacag
    27601 ggtgtcgcct ttgtaaagca tgtcagtctc ctgaatccgc aaggtgatct ggtcgtacca
    27661 gatgcatcga agtgtgtatt ttgtgttaaa aacatgcaaa taaaagatta aagaaatgcc
    27721 gatctgatca cgctcggcag aaatcacgct ctggatgaac gatgtgctaa gatgcggaga
    27781 cttaaggtca aaaaaacaga agggtaaaaa atggaatcac tggcctcact ctataaaaat
    27841 catatagcta ccttacagga acggactcgc gatgcgctgg cgcgcttcaa gctggatgcg
    27901 ttacttattc actccggcga actgttcaac gtttttctcg acgatcatcc ctatccgttt
    27961 aaagtgaacc cgcaattcaa agcgtgggtg ccggtaactc aggtgccaaa ctgctggctg
    28021 ctggtggatg gcgtgaacaa gccgaaactg tggttctatc tgccggttga ttactggcac
    28081 aacgtcgaac cgctgccgac ctctttctgg actgaagacg tggaagtgat cgcgctgccg
    28141 aaagccgatg gcattggtag cctgctgcct gctgcgcgcg ggaatatcgg ttatatcggt
    28201 ccggtgccgg aacgtgtgct gcaactgggt attgaggcca gcaatatcaa cccgaaaggg
    28261 gtgatcgact acctgcatta ctaccgctcc ttcaaaaccg agtacgaact ggcctgtatg
    28321 cgtgaagcgc agaaaatggc ggtcaacggt catcgcgcgg cagaagaagc gttccgttct
    28381 ggcatgagcg agttcgatat caacattgcc tatctgaccg cgaccggtca tcgtgatacc
    28441 gacgtacctt acagcaacat tgtggcgctc aacgaacacg ctgcggtgtt gcattacacc
    28501 aaactggacc accaggcacc ggaagagatg cgcagcttcc tgctggatgc cggggccgaa
    28561 tataacggct atgcggctga cctgacccgt acctggtcgg caaaaagtga caacgactac
    28621 gcacagctgg tgaaagacgt aaatgatgaa caactggcgc tgatcgctac catgaaagca
    28681 ggcgtcagct atgtggatta ccacatccag ttccatcagc gcatcgccaa actgctgcgt
    28741 aaacatcaaa tcatcaccga tatgagtgaa gaagcgatgg tcgaaaacga tctcaccgga
    28801 ccgtttatgc cgcacggtat tggccatccg ctgggcctgc aggtgcatga cgtagccggt
    28861 tttatgcagg atgatagcgg tacgcacctc gcggcaccgg caaaatatcc gtacctgcgc
    28921 tgcacccgta ttctccagcc gggcatggtg ttaaccatcg aaccgggtat ctacttcatt
    28981 gaatcgctgc tggcaccgtg gcgtgaaggg cagttcagca agcacttcaa ctggcagaaa
    29041 attgaagcac tgaaaccgtt cggcggcatt cgtatcgaag acaacgtggt gatccacgaa
    29101 aataacgtgg aaaacatgac ccgggatctg aaactggcgt gatggaaagc tggttaattc
    29161 ctgcggcacc ggtcacggtc gttgaagaga tcaaaaagag ccgcttcata acgatgttgg
    29221 cgcataccga tggcgttgag gcggcgaaag cgtttgttga atcggtgcgg gcagaacacc
    29281 ccgatgcccg ccaccattgc gtggcgtggg tggcgggtgc gccggatgat tcacaacagc
    29341 tgggtttctc tgacgacggg gagccggcgg gaacggcagg taaaccgatg ctcgcccagc
    29401 taatgggcag cggcgttggg gaaattaccg ctgtggtggt gcgctactac ggcggcatat
    29461 tgcttggcac cggtgggtta gtgaaagcgt atggcggcgg cgtgaatcag gcgctgcgcc
    29521 agctaacgac ccaacgcaag acgccattaa ccgaatatac tttgcaatgt gaatatcatc
    29581 agttaaccgg cattgaagcg ttgctggggc agtgtgacgg caaaattatc aacagtgatt
    29641 atcaggcatt cgttctgctg cgggtggcgc ttccggcggc gaaagtggct gaattttccg
    29701 caaagctggc ggattttagc cgtggttcat tgcaattgtt agcgattgaa gaataatccc
    29761 cacttcgttt tgcagaacta aggaagcggc agagatgcat tttcgcgcca ttacccgaat
    29821 cgttggacta ctggtcatct tattttcagg gaccatgatt atccctgggc tggtagcact
    29881 catctaccgg gatggagcgg gccgcgcttt tacccagacc ttttttgtcg ccctcgccat
    29941 tggctctatg ctgtggtggc cgaaccgcaa agagaaaggc gaacttaaat cccgtgaggg
    30001 gtttctgata gtggtgctgt tctggaccgt gctgggtagc gtcggtgcgc tcccttttat
    30061 cttctcggaa agcccgaacc tcacgattac cgatgcgttt tttgaatctt tctctggcct
    30121 gaccaccacc ggggccacta cgctggtggg gctggattcg ctccctcacg ccatcctctt
    30181 ttatcgccag atgctgcaat ggtttggcgg gatggggatc atcgtgttag cggttgcgat
    30241 actgcctatc ctcggcgtgg gtgggatgca gctctatcgc gcagaaatgc ccggcccgct
    30301 gaaagataac aaaatgcgcc cgcgaattgc ggaaacggcg aaaaccctgt ggttgattta
    30361 tgtcttgctg accgtcgcct gtgcgctggc gttgtggttt gccggaatgg atgcctttga
    30421 tgccatcggc catagctttg cgactatcgc tattggcggc ttctcgacac atgatgccag
    30481 tatcggttat ttcgatagcc cgactattaa cactatcatt gctatcttcc tgctgatctc
    30541 cggctgtaac tacggtctgc acttttcact gttaagtggg cgtagtctga aggtttattg
    30601 gcgcgatccg gaatttcgca tgtttatcgg cgtacagttt acgctggtgg ttatttgtac
    30661 cctcgtactg tggtttcata atgtctacag ttcggcgctg atgacaatta accaggcgtt
    30721 tttccaggtg gtgtcgatgg cgacaaccgc cgggtttacg actgacagca ttgcccgctg
    30781 gccgctcttt ttgccggtac tgcttttatg ttcggctttt atcggcggtt gtgccgggtc
    30841 aacgggcggt ggcctgaaag tgatccgcat cctgctgctg tttaagcagg ggaaccgtga
    30901 gctgaaacga ctggtgcatc cgaacgccgt ctatagcatt aagctgggga atcgcgcact
    30961 gccggaacgt atcctcgaag ccgtttgggg atttttctcc gcctatgcat tggtgtttat
    31021 tgtcagtatg ctggcgatta tcgccacggg cgtggatgac ttttctgcct ttgcctcagt
    31081 tgttgcgaca ttgaataacc tggggccagg gcttggcgtg gttgctgata actttaccag
    31141 tatgaacccg gtggctaaat ggatcctgat tgccaacatg ctgtttggtc gtctcgaggt
    31201 ctttacattg ctggtgctct ttaccccgac tttctggcgt gaatgatgga gtaatacgtg
    31261 aaaacattaa ttcttttctc aacaagggac ggacaaacgc gcgagattgc ctcctacctg
    31321 gcttcggaac tgaaagaact ggggatccag acggatgtcg ccaatgtgca ccgcattgaa
    31381 gaaccacaat gggaaaatta tgaccgtgtg gtcattggtg cttctattcg ctatggtcac
    31441 taccactctg cgttccagga atttgtcaaa aaacatgcga cgcgactgaa ttcgatgccg
    31501 agcgcctttt attccgtaaa cctggtggcg cgcaaaccgg agaagcgtac tccacagacc
    31561 aacagctacg cgcggaagtt tctgatgaac tcgcaatggc gtcccgatcg ctgcgcggtc
    31621 attgccgggg cgctgcgtta cccacgttat cgctggtacg accgttttat gatcaagctg
    31681 attatgaaga tgtcaggcgg tgaaacggat acgcgcaaag aagttgtcta taccgattgg
    31741 gagcaggtgg cgaatttcgc ccgagaaatc gcccatttaa ccgacaaacc gacgctgaaa
    31801 taagcacaaa gaataaaaaa tgcgcggtca gaaaattatt ttaaatttcc tcttgtcagg
    31861 ccggaataac tccctataat gcgccaccac tgacacggaa caacggcaaa cacgttgccg
    31921 ggtcagcggg gttctcctga gaatctcaac agagaaaagc aa
//
LOCUS       DAZFQD010000047        31953 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.047, whole genome shotgun sequence.
ACCESSION   DAZFQD010000047 DAZFQD010000000
VERSION     DAZFQD010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31953
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.047"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            162..605
                     /gene="cmtB"
                     /locus_tag="ACHKTW_002215"
     CDS             162..605
                     /gene="cmtB"
                     /locus_tag="ACHKTW_002215"
                     /EC_number="2.7.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IIA"
                     /protein_id="HFO6463237.1"
                     /translation="MRLSDYFPESSISVIHSAKDWQEAIAFSMVSLLDKNYISENYIQ
                     AIKDSTINNGPYYILAPGVAMPHARPECGALKTGMSLTLLEQGVYFPGNDEPIKLLIG
                     LSAADADSHIGAIQALSELLCEEEILEQLLTASSEKQLADIISRG"
     gene            633..2021
                     /gene="cmtA"
                     /locus_tag="ACHKTW_002216"
     CDS             633..2021
                     /gene="cmtA"
                     /locus_tag="ACHKTW_002216"
                     /EC_number="2.7.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HFO6463238.1"
                     /translation="MENKSARAKVQAFGGFLTAMVIPNIGAFIAWGFITALFIPTGWL
                     PNEHFAKIVGPMITYLLPVMIGSTGGHLVGGKRGAVMGGIGTIGVIVGAEIPMFLGSM
                     IMGPLGGLVIKYVDKALEKRIPAGFEMVINNFSLGIAGMLLCLLGFEVIGPAVLIANT
                     FVKECIEALVHAGYLPLLSVINEPAKVLFLNNAIDQGVYYPLGMQQASVNGKSIFFMV
                     ASNPGPGLGLLLAFTLFGKGMSKRSAPGAMIIHFLGGIHELYFPYVLMKPLTIIAMIA
                     GGMSGTWMFNLLDGGLVAGPSPGSIFAYLALTPKGSFLATIAGVTVGTLVSFAITSLI
                     LKMEKTVETESEDEFAQSANAVKAMKQEGAFSLSRVKRIAFVCDAGMGSSAMGATTFR
                     KRLEKAGLAIEVKHYAIENVPADADIVVTHASLEGRVKRVTDKPLILINNYIGDPKLD
                     TLFNQLTAEHKH"
     gene            2036..3313
                     /gene="yggP"
                     /locus_tag="ACHKTW_002217"
     CDS             2036..3313
                     /gene="yggP"
                     /locus_tag="ACHKTW_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFO6463239.1"
                     /translation="MKTKVAAIYGKRDVRLREFELPEITDNELLVSVISDSVCLSTWK
                     AALLGSEHKRVPDDLENHPVITGHECAGVIVEVGKNLTGKYKKGQRFVLQPAMGLPSG
                     YSAGYSYEYFGGNATYMIIPEIAINLGCVLPYHGSYFAAASLAEPMCCIIGAYHANYH
                     TTQYVYEHRMGVKPGGNIALLACAGPMGIGAIDYAINGGIQPSRVVVVDIDDKRLAQV
                     QKLLPVDLAASKGIELVYVNTKGMSDPVQTLRALTGDVGFDDIFVYAAVPAVVEMADE
                     LLAEDGCLNFFAGPTDKNFKVPFNFYNVHYNSTHVVGTSGGSTDDMKEAIALSATGQL
                     QPSFMVTHIGGLDAVPDTVLNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKE
                     LARLVEETHGIWNEQAEKYLLAQFGVDIGEAAQ"
     gene            3310..4275
                     /gene="glpX"
                     /locus_tag="ACHKTW_002218"
     CDS             3310..4275
                     /gene="glpX"
                     /locus_tag="ACHKTW_002218"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HFO6463240.1"
                     /translation="MMSLAWPLFRVTEQAALAAWPQTGCGDKNKIDGLAVTAMRQALN
                     DVAFRGRVVIGEGEIDHAPMLWIGEEVGKGDGPEVDIAVDPIEGTRMVAMGQSNALAV
                     MALAPRDSLLHAPDMYMKKLVVNRLAAGAIDLSLPLADNLRNVARALGKPLDKLRMVT
                     LDKPRLSAAIEEATQLGVKVFALPDGDVAASVLTCWQDNPYDVMYTIGGAPEGVISAC
                     AVKALGGDMQAELIDFCQAKGDYTENRQIAEQERKRCKAMGVDVNRVYSLDELVRGND
                     ILFSATGVTGGELVNGIQQTANGVRTQTLLIGGADQTCNIIDSLH"
     gene            4297..4806
                     /gene="fumE"
                     /locus_tag="ACHKTW_002219"
     CDS             4297..4806
                     /gene="fumE"
                     /locus_tag="ACHKTW_002219"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HFO6463241.1"
                     /translation="MATLTEDDVLEQLDAQDNLFSFMKTAHSILLQGIRQFLPSLFVD
                     NDEEIVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQYWHYLNEQD
                     ETPGFADDMTWDFISNVNSITCNATLYDALKAMKFADFAVWSEARFSGMVKTALTLAV
                     TTTLKELTP"
     gene            4803..5516
                     /locus_tag="ACHKTW_002220"
     CDS             4803..5516
                     /locus_tag="ACHKTW_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HFO6463242.1"
                     /translation="MKIELTVNGLNVQAQYHDEEIERVHKPLLRMLAALQTVSPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLAQQDPELPAIQTLPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLCQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFINAPATALRERLVGRKLAGGVSLADAEAFYDRTDGPNVRRGLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(5488..6165)
                     /locus_tag="ACHKTW_002221"
     CDS             complement(5488..6165)
                     /locus_tag="ACHKTW_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO6463243.1"
                     /translation="MLTLNKISYRWPGAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKVGVLFQEAENQLFHSTVADEIAFG
                     LKLQKCPADEITQRTHAALQCCQLADTANSHPLDLHSAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCGQRGTSVVAISHDAAFTRRHFSRVVRLEDGLIRNVN
                     PPDDIHP"
     gene            complement(6159..6836)
                     /locus_tag="ACHKTW_002222"
     CDS             complement(6159..6836)
                     /locus_tag="ACHKTW_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO6463244.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPGEITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAEAEIMARIDAALTLTECQPLRHRHPATLSGGETQRVVIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWAFERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            complement(6824..7531)
                     /locus_tag="ACHKTW_002223"
     CDS             complement(6824..7531)
                     /locus_tag="ACHKTW_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HFO6463245.1"
                     /translation="MHPFTSLTLWALAACTTLILPAQTILPIYSAATFFCLIALKATR
                     RRAKYVVWLMFSLGAGLWLVHGGWLPEWLSGTPRSPERWSHAITLWLRILAIVSTSQL
                     WMQYVPVQCFIRALFASRLPLGVAYLFAGPLLVVEQLKRQLAIIHEAQRARGVPLDEG
                     WYQRLRAMPALIIPLTHNALNNLAVRGAALDMRAFRIQNRRTTLWAPADSTLQRVARY
                     TMILLMLTEFGAWIWLR"
     gene            complement(7532..8110)
                     /locus_tag="ACHKTW_002224"
     CDS             complement(7532..8110)
                     /locus_tag="ACHKTW_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFO6463246.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVAGWLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTELFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            complement(8133..8564)
                     /locus_tag="ACHKTW_002225"
     CDS             complement(8133..8564)
                     /locus_tag="ACHKTW_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing DNA-binding protein"
                     /protein_id="HFO6463247.1"
                     /translation="MMTLPYPYSSSARFYALRLLPGQEVFSQLHAFAQQHQLHAAWIA
                     GCTGSLTDVALRYAGQEGTTLLNGTFEVISLNGTLEQSGEHLHLCVSDPHGTMLGGHM
                     MPGCTVRTTLELVIGSLEELAFSRQPCALSGYDELHISPVK"
     gene            8936..9955
                     /gene="epd"
                     /locus_tag="ACHKTW_002226"
     CDS             8936..9955
                     /gene="epd"
                     /locus_tag="ACHKTW_002226"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048001 - erythrose-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HFO6463248.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            10005..11168
                     /gene="pgk"
                     /locus_tag="ACHKTW_002227"
     CDS             10005..11168
                     /gene="pgk"
                     /locus_tag="ACHKTW_002227"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFO6463249.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            11383..12462
                     /gene="fbaA"
                     /locus_tag="ACHKTW_002228"
     CDS             11383..12462
                     /gene="fbaA"
                     /locus_tag="ACHKTW_002228"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFO6463250.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            complement(12526..12624)
                     /locus_tag="ACHKTW_002229"
                     /pseudo
     CDS             complement(12526..12624)
                     /locus_tag="ACHKTW_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            12820..13680
                     /gene="mscS"
                     /locus_tag="ACHKTW_002230"
     CDS             12820..13680
                     /gene="mscS"
                     /locus_tag="ACHKTW_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HFO6463251.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            13819..14454
                     /gene="argO"
                     /locus_tag="ACHKTW_002231"
     CDS             13819..14454
                     /gene="argO"
                     /locus_tag="ACHKTW_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0061459 - L-arginine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HFO6463252.1"
                     /translation="MFSYYFQGLALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVAFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKAQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            14547..15287
                     /gene="yggE"
                     /locus_tag="ACHKTW_002232"
     CDS             14547..15287
                     /gene="yggE"
                     /locus_tag="ACHKTW_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HFO6463253.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            15454..16350
                     /gene="ygfI"
                     /locus_tag="ACHKTW_002233"
     CDS             15454..16350
                     /gene="ygfI"
                     /locus_tag="ACHKTW_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417396.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO6463254.1"
                     /translation="MDIFISKKMRNFILLAQTNNIARAAEKIHMTASPFGKSIAALEE
                     QIGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRSREIVIGIDNT
                     YPTIIFDQLISLGDKYEGVTAQPVEFSENGVIDNLFDRQLDFIISPQHVSARVQELEN
                     LTISELPPLRLGFLVSRRYEERQEQELLQELPWLQMRFQNRANFEAMIDANMRPCGIN
                     PTIIYRPYSFMAKISAVERGHFLTVIPHFAWRLVNPATLKYFDAPHRPMYMQEYLYSI
                     RNHRYTATMLQHIAEDRDGTSH"
     gene            complement(16347..17825)
                     /gene="scpC"
                     /locus_tag="ACHKTW_002234"
     CDS             complement(16347..17825)
                     /gene="scpC"
                     /locus_tag="ACHKTW_002234"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
                     /protein_id="HFO6463255.1"
                     /translation="METQWTRMTADEAAEIIQHNDMVAFSGFTPAGSPKALPTAIARR
                     ANEQHEAKKPYQIRLLTGASISAAADDVLSDADAVSWRAPYQTSSGLRKKINQGAVSF
                     VDLHLSEVAQMVNYGFFGDIDVAVIEASALAPDGRVWLTSGIGNAPTWLLRAKKVIIE
                     LNHYHDPRVAELADIVIPGAPPRRNSVSIFHAMDRVGTRYVQIDPKKIVAVVETNLPD
                     AGNMLDKQNPMCQQIADNVVTFLLQEMAHGRIPPEFLPLQSGVGNINNAVMARLGENP
                     EIPPFMMYSEVLQESVVHLLETGKISGASASSLTISADSLRKIYDNMDYFASRIVLRP
                     QEISNNPEIIRRLGVIALNVGLEFDIYGHANSTHVAGVDLMNGIGGSGDFERNAYLSI
                     FMAPSIAKEGKISTVVPMCSHVDHSEHSVKVIITEQGIADLRGLSPLQRARTIIDNCA
                     HPMYRDYLHRYLENAPGGHIHHDLSHVFDLHRNLIATGSMLG"
     gene            complement(17849..18634)
                     /gene="scpB"
                     /locus_tag="ACHKTW_002235"
     CDS             complement(17849..18634)
                     /gene="scpB"
                     /locus_tag="ACHKTW_002235"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HFO6463256.1"
                     /translation="MSYQYVNVVTINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            complement(18645..19640)
                     /gene="meaB"
                     /locus_tag="ACHKTW_002236"
     CDS             complement(18645..19640)
                     /gene="meaB"
                     /locus_tag="ACHKTW_002236"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HFO6463257.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPYCGNALRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGVGQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLLVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRVLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLHHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            complement(19633..21777)
                     /gene="scpA"
                     /locus_tag="ACHKTW_002237"
                     /pseudo
     CDS             complement(19633..21777)
                     /gene="scpA"
                     /locus_tag="ACHKTW_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417392.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
     gene            complement(21981..22874)
                     /gene="argP"
                     /locus_tag="ACHKTW_002238"
     CDS             complement(21981..22874)
                     /gene="argP"
                     /locus_tag="ACHKTW_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HFO6463258.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            23016..23201
                     /locus_tag="ACHKTW_002239"
     CDS             23016..23201
                     /locus_tag="ACHKTW_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001328908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6463259.1"
                     /translation="MHFAQRVRALVVLNGVALLPLFAPWSGIPRPLYALFAGRKGMPA
                     IARYFMDELTTRLANGV"
     gene            23257..23916
                     /gene="rpiA"
                     /locus_tag="ACHKTW_002240"
     CDS             23257..23916
                     /gene="rpiA"
                     /locus_tag="ACHKTW_002240"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFO6463260.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            24172..25404
                     /gene="serA"
                     /locus_tag="ACHKTW_002241"
     CDS             24172..25404
                     /gene="serA"
                     /locus_tag="ACHKTW_002241"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFO6463261.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            25633..25692
                     /gene="ibsC"
                     /locus_tag="ACHKTW_002242"
     CDS             25633..25692
                     /gene="ibsC"
                     /locus_tag="ACHKTW_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HFO6463262.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            26025..26084
                     /locus_tag="ACHKTW_002243"
     CDS             26025..26084
                     /locus_tag="ACHKTW_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFO6463263.1"
                     /translation="MMRLAIILIVLLLISFPAY"
     gene            complement(26185..26787)
                     /gene="fau"
                     /locus_tag="ACHKTW_002244"
     CDS             complement(26185..26787)
                     /gene="fau"
                     /locus_tag="ACHKTW_002244"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFO6463264.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQQEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(26808..26991)
                     /gene="ssrS"
                     /locus_tag="ACHKTW_002245"
     ncRNA           complement(26808..26991)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACHKTW_002245"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(27033..27362)
                     /gene="zapA"
                     /locus_tag="ACHKTW_002246"
     CDS             complement(27033..27362)
                     /gene="zapA"
                     /locus_tag="ACHKTW_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFO6463265.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            27530..28108
                     /gene="ygfB"
                     /locus_tag="ACHKTW_002247"
     CDS             27530..28108
                     /gene="ygfB"
                     /locus_tag="ACHKTW_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HFO6463266.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            28134..29459
                     /gene="pepP"
                     /locus_tag="ACHKTW_002248"
     CDS             28134..29459
                     /gene="pepP"
                     /locus_tag="ACHKTW_002248"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HFO6463267.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYTYADEIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPIVHEMRLFKSPEEIAVLRRAGEITALAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENECELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSMQEVTGEVVRIMVSGLVKLGILKG
                     DVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     AEVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            29456..30634
                     /gene="ubiH"
                     /locus_tag="ACHKTW_002249"
                     /gene_synonym="visB"
     CDS             29456..30634
                     /gene="ubiH"
                     /locus_tag="ACHKTW_002249"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008681 - 2-octaprenyl-6-methoxyphenol
                     hydroxylase activity [Evidence IEA];
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HFO6463268.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADCATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVTLHCPDRVANVARTQSHVEVTLESGEMLTGRVLIA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGKITHAGKRSAYPLALTRAARA
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAHERGEDIGDYGILCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            30657..31859
                     /gene="ubiI"
                     /locus_tag="ACHKTW_002250"
                     /gene_synonym="visC"
     CDS             30657..31859
                     /gene="ubiI"
                     /locus_tag="ACHKTW_002250"
                     /gene_synonym="visC"
                     /EC_number="1.14.13.240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HFO6463269.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAADA
                     PPQLRVSAINAASEKLLTRLGVWQDILSRRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPAELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLVLVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGTNPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
BASE COUNT         7656 a         7914 c         8596 g         7787 t
ORIGIN      
        1 ttgacatttg ttgttatatc tataactaaa tgttatataa gaaattaata attcactgtt
       61 ttcaaaacat cggtttccct gctcaattgc tttcattaaa ccgcacgagt attttatttt
      121 taccgccgga cggtacccta aaaataaaat aaggataatt tatgcggctt agtgattatt
      181 ttccagaatc atcaatctca gttatacatt cagcaaaaga ttggcaggaa gctatcgctt
      241 tctcgatggt atcattgctg gataaaaact atatcagcga gaattacatt caggcaatta
      301 aagattccac cattaacaat ggcccttatt atattctcgc accaggcgtg gcaatgcctc
      361 atgcgcgacc ggaatgtggg gcgcttaaaa ccgggatgtc tttgacatta cttgaacaag
      421 gtgtttattt tccggggaat gacgaaccaa ttaaattact catcggactc tctgctgccg
      481 atgccgattc gcacattggc gctattcagg cgttaagtga attactgtgc gaagaagaaa
      541 tactcgaaca actcttaaca gcatcatcag aaaaacaatt agcggacatt atcagccgcg
      601 gataattact ctctcctcta caggataata ttatggaaaa caagtctgct cgtgcaaagg
      661 tccaggcttt tgggggcttt ttgactgcaa tggtcatccc caatattggt gcttttattg
      721 cctggggttt tattactgcg ttatttattc ccaccggttg gctgcctaac gaacatttcg
      781 ccaaaattgt cggcccgatg attacctatt tattgcccgt gatgattggt tctacaggtg
      841 gtcatctggt cggcggtaaa cgcggggcgg tcatgggcgg aataggtact attggtgtga
      901 tcgttggcgc agagatcccg atgttccttg gctcaatgat catggggccg ctcggtgggt
      961 tggtcataaa atatgtcgat aaggcactgg aaaaacgcat acctgccggt tttgagatgg
     1021 ttatcaataa cttctcatta ggtatcgcgg ggatgctcct ttgtctgctg ggttttgaag
     1081 tgatcggccc ggcggtgtta attgccaata ctttcgtcaa agagtgtatc gaggcgctgg
     1141 tacatgcggg ttatctgcct ctgctgtcag tcatcaatga accggcgaaa gtgcttttcc
     1201 ttaataatgc gatcgatcag ggcgtctatt atccgctggg aatgcaacag gcttcggtta
     1261 acggtaaatc catcttcttt atggtggcct ctaacccagg tccgggcctg gggctgctgc
     1321 tggcgtttac cttgtttggt aaagggatga gtaaacgttc tgcgcccggg gcgatgatta
     1381 ttcacttcct cggtgggatc cacgaactgt atttcccata tgtgctgatg aagccgctga
     1441 ctattattgc catgattgcg ggcggtatgt ctggtacctg gatgtttaac ttactggacg
     1501 gtggtctggt ggctggccca agtccggggt ctatctttgc ttacctggca ctgacgccga
     1561 aaggctcgtt cctggcgaca attgccggtg ttacggtagg taccctggtg tcctttgcta
     1621 ttacttcgct gatactgaag atggaaaaaa cggtggaaac ggagagcgaa gatgagtttg
     1681 ctcagtcagc caatgcggtt aaggcgatga aacaagaggg tgcattctcg ttaagcaggg
     1741 ttaagcgtat cgcctttgtt tgcgatgcgg ggatgggctc cagtgcgatg ggcgcgacca
     1801 ccttccgtaa acgcctggaa aaagcggggc tggcaataga agtaaaacat tacgcgatag
     1861 aaaacgtgcc tgcggatgcc gatatcgtcg ttactcatgc cagtctggaa gggcgcgtga
     1921 aacgtgttac ggataaaccg ctgatattga ttaataacta tattggcgat ccaaaactcg
     1981 acactttatt taatcaatta accgccgaac ataaacactg attacagagg taaaaatgaa
     2041 aaccaaagtt gctgctattt atggcaagcg ggatgtccgt ctgcgcgaat ttgaactgcc
     2101 agaaattacc gataatgaac tgttagtgag tgtaatttct gacagcgtct gtttatcgac
     2161 ctggaaagcg gcgttactcg gtagtgaaca taaacgcgta cccgacgatt tagaaaatca
     2221 tccggtcatt accgggcatg aatgtgccgg ggttattgtc gaagtgggta aaaatctcac
     2281 tggcaaatat aaaaaaggcc agcgttttgt attgcaaccg gcgatggggt taccaagcgg
     2341 atattcagca ggctacagct acgaatattt tggcggtaac gccacctata tgattattcc
     2401 cgaaatcgcc attaatttgg gctgcgtatt accgtatcac ggctcttatt ttgctgcggc
     2461 gtcgctggca gagcctatgt gctgcattat tggtgcttat catgccaatt atcacaccac
     2521 gcaatatgtt tatgagcatc gcatgggcgt caaacctggc ggcaatattg cactgctggc
     2581 gtgtgcgggt ccgatgggca ttggcgctat cgattacgcc attaacggtg gcatacaacc
     2641 gtcacgggtg gtggtggtcg atatcgacga caaacgcctg gcgcaggtgc agaagctgct
     2701 gccggtggat ctggcggcca gtaaaggcat tgagctggtg tatgtgaata ccaaagggat
     2761 gagcgatccg gtccagacgc tgcgggcgct gacaggagat gtcgggttcg atgacatttt
     2821 tgtttatgcg gcggtgcctg ctgtcgttga gatggctgac gaattactgg cggaagatgg
     2881 ctgtctgaac ttctttgccg ggccgacgga taaaaacttc aaagtgccgt ttaatttcta
     2941 caacgtccat tacaacagca cgcacgtagt cggtacatcc ggcggttcaa cggacgacat
     3001 gaaagaggcg attgcgttaa gcgccacggg gcagttgcag ccgtccttta tggtcacgca
     3061 tatcggtggg ctggatgcgg tgccagatac cgtgctcaat ctgccggata tccctggcgg
     3121 taaaaaactc atttataacg gcgtgaccat gccgctcact gccattgccg attttgccga
     3181 aaaaggcaaa accgatccgc tgtttaaaga gttggcgcgg ctggttgagg aaacgcacgg
     3241 catctggaat gaacaggccg agaaatatct gctggcacaa tttggcgttg atatcgggga
     3301 ggccgcgcaa tgatgtccct ggcgtggcca ttatttcgcg ttacggaaca ggcagcgttg
     3361 gctgcctggc cgcaaaccgg atgtggcgac aaaaataaaa ttgatggcct ggcggtcacc
     3421 gcgatgcgcc aggcattaaa cgacgtcgct tttcgtggac gagtggttat cggcgaagga
     3481 gaaattgacc atgcgccgat gctgtggatt ggcgaagagg tgggcaaagg cgatgggcca
     3541 gaagtcgata tcgcggttga cccgattgaa ggcacgcgga tggtagcgat ggggcagagc
     3601 aatgcgctgg cggtcatggc tttagcccct cgagatagcc tgttacatgc gcccgatatg
     3661 tatatgaaaa agctggttgt taatcgactg gctgccgggg cgattgatct gtctttgcct
     3721 ctggcggaca acttgcgtaa cgtggcgcgg gcgttaggta aaccgctgga caagctgcgc
     3781 atggttacgc tggataaacc gcgcctgagt gccgcgattg aagaagcaac acagctgggc
     3841 gtgaaggttt tcgctctgcc ggatggcgat gtcgccgcca gcgtgctgac ctgctggcag
     3901 gataatcctt acgacgtgat gtacaccatc ggcggtgcgc cagaaggcgt gatttctgcc
     3961 tgtgccgtta aagcattagg tggggatatg caggccgagc tgattgactt ttgtcaggct
     4021 aaaggggatt acacggaaaa tcggcagatt gcagagcagg agcgcaagcg ttgtaaggca
     4081 atgggcgttg acgttaaccg tgtgtactcg ctcgatgaac tggtgagggg gaacgatatc
     4141 ctctttagcg ctacgggcgt gacgggcggc gagttggtga acggtatcca acagacggcg
     4201 aatggggtgc ggacgcagac attactgatc ggcggcgcgg accaaacgtg taatataata
     4261 gactccctgc attgatggtg atctatcgga agaccaatgg cgacgctgac agaagatgat
     4321 gtgcttgagc aactggatgc acaggacaat ttattttcat ttatgaaaac tgcgcattct
     4381 attttgctcc aggggatacg ccagtttctg ccgtcgctgt ttgtcgataa cgatgaagag
     4441 atcgtcgaat atgcagtgaa gccgttactc gcccaaagcg gcccgcttga cgatattgat
     4501 gttgcgctgc gtttgattta tgcgctgggg aaaatggata aatggctgta tgccgatatc
     4561 acgcactttt cccagtactg gcattacctg aacgaacagg atgaaacgcc cggatttgcc
     4621 gatgacatga cctgggattt tatcagcaac gtcaatagca taacctgcaa tgcgacgctc
     4681 tatgacgcgt taaaagcgat gaagttcgcc gatttcgccg tctggtcaga ggcgcgtttt
     4741 agcggaatgg tcaaaacggc gctgacgctg gcagtaacga ccaccttaaa ggaattaacg
     4801 ccgtgaaaat tgaattaacg gtgaatgggc tgaacgtgca agcgcagtac catgatgaag
     4861 agatagaacg cgtgcataaa ccgttgctgc gtatgctggc ggccttacag acggtaagtc
     4921 cgcagcgacg tacggtggtt tttctttgcg ctccgccggg gacgggtaaa tctaccttaa
     4981 ctaccttctg ggaatatctc gcgcaacaag accctgaatt acccgctatc cagacgctcc
     5041 cgatggatgg ttttcaccat tacaatagct ggctggatgc gcatcaattg cgccccttca
     5101 aaggcgcacc agagacattt gacgttgcga aactggcgga aaatctgtgc caggtagtgg
     5161 aaggggattg tacgtggccg cagtatgatc gacaaaagca tgatcctgtt gaagatgcgt
     5221 tgcacgttac cgcaccactc gtcatcgtcg aaggaaactg gttgttgctg gacgacgaga
     5281 agtggtgcca gcttgcgcaa ttttgtgatt tctcgatctt tatcaacgcg ccagctactg
     5341 cgttgcggga acgactggta gggcgcaagc tggcgggagg ggtatcgctg gcagacgcag
     5401 aagcttttta tgaccgtacc gacgggccga atgttcgccg ggggctggaa gagagtctgc
     5461 ctgcaaattt gaccttaatg atgaccgcta cgggtgaata tcgtctggtg gattaacgtt
     5521 cctgattaga ccatcttcca gtcgcaccac acgagagaaa tgacgccggg taaatgcggc
     5581 gtcatgactg attgctacga cgctcgtgcc tcgctggccg cacttctcca gccagctttc
     5641 aaacacgctc aaccaatttt cgtcaaaatc ccgactcggc tcgtccagca gcaatagtgg
     5701 cggagagagt gcttccaggc aggcgacggc gaccatccgt cgctgcgcac tatgcagatc
     5761 caacgggtgc gagttggcag tgtctgctaa ctgacaacat tgcagggcag catgggtacg
     5821 ttgagtgatt tcatccgccg ggcatttttg cagtttcaaa ccaaatgcga tctcgtcagc
     5881 gacggtgctg tgaaaaagtt ggttttccgc ttcctgaaac agtacgccaa cctttgccgc
     5941 gcgctggcgg tttttcaggt ttttcatcgc ctgttgttgc agcataaccg taccggctgt
     6001 gggcgtaaga agacctgcca ttactcgtaa cagcgtcgat tttcccgcgc cattgtcgcc
     6061 ggtcagcgcc aaccattcac cttgtttgag ttgcagagaa atgtcgcaaa gacaatccgt
     6121 tgccgcgcca ggccagcgat aagatatctt atttaacgtt aacatagtgg ctggagcgct
     6181 ccgttttgca attgccacgc ttgctggcag tagttcagga aaggtgtgcg atgacgttca
     6241 aaaagaataa tcagcgaacc acgttcaaac gcccagtgtt gtaagcgctg caacagcatt
     6301 tcgctggcct gcggagtcag gcggctaaag gcttcatcaa gaattaagag ctttggctgc
     6361 atcgcgatgg cgcaggcgat gaccacgcgc tgtgtctctc caccggagag tgttgccggg
     6421 tgacgatggc gcaacggctg gcactcggtc agagtcaggg cggcatcaat ccgcgccata
     6481 atctctgctt cagcaagaca cagattttct ggcccaaagg caacttcttc ttccacgcta
     6541 aaggtacagc cggagagctg caaatagggc gattgctgga cgagctggat ggtggcggat
     6601 tgttcattaa gcgggagatg cccaatgggc gtaccgagga gcgtccccgt gcctgttatc
     6661 tctcctggca gaaagtccgg ataccagccc gccatcagct gtgccagcgt acttttgcca
     6721 ctgccgttgt caccaaagat ggcgaccatc cccggtttga catagtgaaa gtcataacag
     6781 aacggaggat gcgttgagtg tgtcgggcaa taacggaact gttctaacgt aaccatatcc
     6841 acgctccgaa ttctgtcagc atcagcaaga tcatggtgta acgcgctacg cgttgtaggg
     6901 tgctatcggc tggtgcccac aacgttgttc gtcggttttg gatccgaaat gcccgcatat
     6961 ccagcgccgc accacggaca gcaagattgt ttagcgcatt gtgtgttagc ggaataatta
     7021 acgcgggcat cgcacgtaaa cgctgatacc agccctcatc cagcggcacg ccgcgggcac
     7081 gctgcgcttc atgaataatc gctaattgcc gttttaactg ctcaacgacc aataacggcc
     7141 cggcaaacag ataagcgacg cctagcggca ggcgagaagc aaacagagcg cggataaaac
     7201 attgcacggg gacgtactgc atccacaact gtgaggttga aacaatcgcc agaatccgca
     7261 accagagcgt aatagcatgc gaccaccgtt ctggcgagcg tggcgtgccg cttagccatt
     7321 ctggtagcca gccaccgtgt accagccaca gcccggctcc cagcgagaac atcagccaga
     7381 cgacatattt tgcccgccgc cgcgtggcct ttagcgcaat aaggcagaaa aaggtcgccg
     7441 cgctatagat cggcaaaatg gtctgcgcgg gcaggataag cgtggtgcag gccgccaatg
     7501 cccacagtgt taatgaggta aatggatgca tttagcgcac ggcagccatt gccggaaaat
     7561 ggcgcgtggt gcgaatcggc agctggcgca gtaaaagcca gacaatcacc gctgtaagaa
     7621 ttttatcgac caggttcgcg cctatcaccg taatcgccac cgactccacc aggttttgcc
     7681 ccatcgagtg catccaggca acgaacaaat cggctccgct accggtgaca ccgccgaaca
     7741 gctcagtacg cagcggtaca gcgacgaccg ttaccgcaag cgtaataatc acgccgctga
     7801 caacgacttt tggcagggtg cgaaaccaac ccgctcgcgc cagccaaccg gcgaccaggc
     7861 cgatgaccat cgcgacgggt gcaaatgcgg cggcgatcgg gtcggtcagc aatccccaga
     7921 gtaaattggt cagtaagccg gtcagcattc cgatcaccgg accaagcagt accgcgctaa
     7981 taagtgtgcc gatggagtcg agaaatatag gtaatttgac catgctggcc agttgcccgc
     8041 cgatcatatt gatggcgata gatataacga tcaacaccag tgcctgactg gaaaagtggc
     8101 tacgcgccat aagaaaacct ctttgaaagt agttatttaa cgggggaaat atgcaactcg
     8161 tcgtaaccag aaagcgcgca aggttggcgg ctaaacgcca gttcctcaag gctaccgatc
     8221 actagttcaa gcgtggtccg cacggtacag ccgggcatca tatgaccgcc aagcatcgtg
     8281 ccgtgtggat cggaaacgca gagatgaaga tgctcaccac tctgttccag cgtgccgttt
     8341 agtgagatca cttcgaatgt accgttcagg agcgtggtgc cttcttgccc ggcatagcgc
     8401 aaggcgacgt cagtcaggct accggtacag cctgcaatcc aggcggcatg aagttgatgc
     8461 tgctgtgcaa aagcgtgtag ctgggaaaaa acttcctgcc cggggaggag gcgcagtgcg
     8521 taaaagcgtg cggaggaaga atatgggtag ggcaatgtca tcattggagt actccgaaac
     8581 tcggacgtat tgcggataat atcgacgctg catcagtcat attctgatat aagtcagggt
     8641 ttgtgacggc acaacgaaaa gattttgcta cgcttattcg tgattctgca aagtggaata
     8701 aattccacag gggcggggct gaaaacgtga cgtaagtcag ataattgctt tctttgactg
     8761 gacaatcatt ccttttattc cacgtttcgc ttatcctagc tgaagcgttt cagtcgatta
     8821 aatgttcgac aattaaccaa tcagtcgcag tttgcgacag gtaaggtttc cccggacgat
     8881 ttgctggatt actctgtctg gcaaatttgc tcagggaaaa ccttgcagga gatctatgac
     8941 cgtacgcgta gcgataaatg gcttcggtcg catcgggcgt aatgtggttc gtgctttgta
     9001 tgaatcagga cgccgggcgg aaattaccgt ggtggcaatc aacgaactgg cggatgctgc
     9061 gggcatggcg catttgttga aatatgacac cagccatggc cgttttgcat gggaagtacg
     9121 acaggaacgc gatcaacttt ttgttggtga tgacgccatc cgcgtattgc atgaacgttc
     9181 actgcaatcg ctcccctggc gtgaacttgg cgttgatgta gtcctcgact gcaccggcgt
     9241 atatggctcc cgcgagcatg gcgaagcgca tattgccgcc ggggccaaaa aagtgctctt
     9301 ttcacatcct ggcagtaacg atctcgacgc gaccgttgtt tacggcgtca atcaggatca
     9361 acttcgtgcg gaacaccgca tcgtttctaa cgcttcctgt accacgaatt gcataattcc
     9421 cgtcatcaaa ttgttagatg atgcgtacgg tattgagtcc ggcacagtga ccacaattca
     9481 ctccgccatg cacgatcaac aggttattga tgcataccat cctgacctgc gtcgcacccg
     9541 ggcagccagc cagtcgatca ttccggtcga tactaaactg gccgccggta tcacacgatt
     9601 ttttccgcaa tttaacgatc gctttgaagc gattgcggta cgtgtgccaa ccataaatgt
     9661 gacggcaatc gatttaagcg tgacggtgaa gaaacctgta aaagccaatg aagtcaacct
     9721 gttgctgcaa aaagcagcac aaggtgcatt tcatggtata gttgactata cggaattgcc
     9781 gttggtctct gtagatttta accacgatcc gcacagtgcc attgtcgatg gcacccaaac
     9841 ccgggtcagt ggcgcacacc tgatcaaaac gttggtctgg tgcgataacg aatggggctt
     9901 tgctaaccga atgctcgaca cgacgttagc tatggctact gttgctttca ggtaagacgc
     9961 aagcagcgtc tgcaaaactt ttagaatcaa cgagaggatt caccatgtct gtaattaaga
    10021 tgaccgatct ggatcttgct gggaaacgtg tatttatccg tgcggatctg aacgtaccag
    10081 taaaagacgg gaaagtaacc agcgacgcgc gtatccgtgc ttctctgccg accatcgaac
    10141 tggccctgaa acaaggcgca aaagtgatgg taacttccca cctgggtcgt cctaccgaag
    10201 gcgagtacaa cgaagaattc tctctgctgc cggttgttaa ctacctgaaa gacaaactgt
    10261 ctaacccggt tcgtctggtt aaagattacc tcgacggcgt tgacgttgct gaaggtgaac
    10321 tggttgttct ggaaaacgtt cgcttcaaca aaggcgagaa gaaagacgac gaaaccctgt
    10381 ccaaaaaata cgctgcactg tgtgacgtgt tcgtaatgga cgcattcggt actgctcacc
    10441 gcgcgcaggc ttctactcac ggtatcggta aattcgctga cgttgcgtgc gcaggcccgc
    10501 tgctggcagc tgaactggac gcgctgggta aagcactgaa agaacctgct cgcccgatgg
    10561 tggctatcgt tggtggttct aaagtatcta ccaaactgac cgttctggac tccctgtcta
    10621 aaatcgctga ccagctgatt gttggtggtg gtatcgctaa cacctttatc gcggcacaag
    10681 gccacgatgt gggtaaatcc ctgtacgaag ctgacctggt tgacgaagct aaacgtctgc
    10741 tgaccacctg caacatcccg gttccgtctg atgttcgcgt agcaaccgag ttctctgaaa
    10801 ctgcaccggc taccctgaaa tctgttaacg atgtgaaagc tgacgagcag atcctggata
    10861 tcggtgatgc ttccgctcag gaactggctg aaatcctgaa gaatgcgaaa accattctgt
    10921 ggaacggtcc ggttggcgtg ttcgaattcc cgaacttccg caaaggtact gaaatcgtgg
    10981 ctaacgctat cgcagacagc gaagcgttct ccatcgctgg cggcggcgac actctggcag
    11041 caatcgacct gttcggcatt gctgacaaaa tctcctacat ctccactggc ggcggcgcat
    11101 tcctcgaatt cgtggaaggt aaagtactgc ctgcagtagc gatgctcgaa gagcgcgcta
    11161 agaagtaaaa aatcacaggg cagggaaacc tgcccttgtt tcagcgcgct tttagagcac
    11221 gcattttttc atactctaaa taattcgagt tgcaggaagg cgacaagaga gtgaatcccc
    11281 aggagcttac ataagtaagt gactggggtg agcgaacgca gacgcagcac atgcaacttg
    11341 aagtatgacg agtataaggc ccgacgatac aggataagag acatgtctaa gatttttgat
    11401 ttcgtaaaac ctggcgtaat cactggtgat gacgtacaga aagttttcca ggtagcaaaa
    11461 gaaaacaact tcgcactgcc agcagtaaac tgcgtcggta ctgactccat caacgccgta
    11521 ctggaaaccg ctgctaaagt taaagcgccg gttatcgttc agttctccaa cggtggtgct
    11581 tcctttatcg ctggtaaagg cgtgaaatct gacgttccgc agggtgctgc tatcctgggc
    11641 gcgatctctg gtgcgcatca cgttcaccag atggctgaac attatggtgt tccggttatc
    11701 ctgcacaccg accactgcgc gaagaaactg ctgccgtgga tcgacggtct gctggacgcg
    11761 ggtgaaaaac acttcgctgc taccggcaag ccgctgttct cttctcacat gatcgacctg
    11821 tctgaagaat ctctgcaaga gaacatcgaa atctgctcta aatacctgga gcgtatgtcc
    11881 aaaatcggca tgactctgga aatcgaactg ggttgcaccg gtggtgaaga agacggcgtg
    11941 gacaacagcc acatggacgc ttctgcactg tacacccagc cggaagacgt tgattacgca
    12001 tacaccgagc tgagcaaaat cagcccgcgt ttcaccatcg cagcgtcctt cggtaacgta
    12061 cacggtgttt acaagccggg taacgtggtt ctgactccga ccatcctgcg tgattctcag
    12121 gaatatgttt ctaagaaaca caacctgccg cacaacagcc tgaacttcgt attccacggt
    12181 ggttccggtt ctactgctca ggaaatcaaa gactccgtaa gctacggcgt agtgaaaatg
    12241 aacatcgata ccgataccca atgggcaacc tgggaaggcg ttctgaacta ctacaaagcg
    12301 aacgaagctt atctgcaggg tcagctgggt aacccgaaag gcgaagatca gccgaacaag
    12361 aaatactacg atccgcgcgt atggctgcgt gccggtcaga cttcgatgat cgctcgtctg
    12421 gaaaaagcat tccaggaact gaacgcgatc gacgttctgt aagatattcc tttctgctta
    12481 tctcaaggcc cgctctgcgg gtcttttttt cgccaaaagc aaaattgcct gatgcgctac
    12541 gcttatcagg cctacacgta tcctgcaata tattgaattt gcaggttttt gtaggccgga
    12601 taaggcgttc acgccgcatc cggcatgaca aagcgcacat tgatatctgt ctgagccccg
    12661 ctctgcgggg attttttcgc caaaagcaag gtgattcaga tgagaaatct gtgatctatt
    12721 tggcaaaatt atgctttatt gtttaccctt gtcagactgc ccgtcataag gcggcggagt
    12781 gtatttctcc attttgagtc agttgaaaag gaatattgaa tggaagattt gaatgttgtc
    12841 gatagcataa acggcgcggg aagctggctg gtagctaacc aggcgctgct gctaagttat
    12901 gcagtaaaca tcgtggcggc actcgcgatc atcatcgttg gtttgattat cgcgcggatg
    12961 atttccaacg cggtgaatcg cctgatgatc tcccgtaaaa tcgatgccac tgttgctgat
    13021 tttctctctg cattagtccg ttacggtatt atcgccttta cgctaatcgc tgcactggga
    13081 cgcgtgggtg tacaaaccgc gtccgtcatt gctgtactcg gtgccgcagg cttagctgtt
    13141 ggtctggctt tgcaggggtc actgtctaac ctggccgctg gcgtgttact tgtcatgttc
    13201 cgcccgttcc gtgccggaga atatgttgac ctgggcggcg tagccggtac tgtgctgagt
    13261 gtgcagattt tctccaccac catgcgtact gcagacggta aaattatcgt tattccgaac
    13321 ggtaaaatta ttgccggaaa tattattaac ttctcccgcg agccagttcg ccgtaacgaa
    13381 tttattattg gcgtggcgta tgattccgat atcgatcagg ttaagcagat cctgaccaat
    13441 attatccagt ctgaagatcg cattttgaaa gatcgcgaaa tgactgtgcg cctgaacgaa
    13501 cttggtgcat cgtcgattaa tttcgtggtc cgcgtctgga gcaacagcgg cgatctgcaa
    13561 aacgtgtact gggatgtgct ggagcgtatt aaacgtgaat ttgatgccgc cggtatcagc
    13621 ttcccgtacc cgcaaatgga tgtgaacttt aagcgggtga aagaagacaa agctgcgtaa
    13681 tcaacgctga ggccagataa tactctctgg cctctctctt attagttatt ctgattgcca
    13741 attaatatta tcaatttccg ctaataacaa tcccgcgata tagtctctgc atcagatact
    13801 taattcggaa tatccaacgt gttttcttat tactttcaag gtcttgctct tggggcggct
    13861 atgatcctac cgctcggtcc acaaaatgct tttgtgatga atcagggcat tcgtcgtcag
    13921 taccacatta tgattgcctt actttgtgct atcagcgatt tggtcctgat ttgcgcgggg
    13981 atttttggtg gcagcgcgtt attgatgcag tcgccgtggt tgctggcgct ggtcacctgg
    14041 ggcggcgtag ccttcttgct ctggtatggt tttggcgctt ttaaaacagc aatgagcagt
    14101 aatattgagt tagccagtgc cgaagtcctt aagcaaggca gatggaaaat tatcgccacc
    14161 atgctggcag tgacctggct gaatccgcat gtttacctgg atacttttgt tgtactgggc
    14221 agcctcggcg ggcaacttga cgtcgaacca aaacgttggt ttgcgctcgg gacaattagt
    14281 gcctctttct tgtggttctt tggtctggct attctcgcag cctggctggc accgcgtctg
    14341 cgcacagcaa aagcacagcg cattatcaat ctggtagtgg gatgtgttat gtggtttatt
    14401 gccttgcagc tggcgagaga cggtattgct catgcacaag ccttgttcag ttaggcgcta
    14461 tctgatggaa aaataaaaca gaggcgctaa gcttgcctcc agaggtcctg aattttgggc
    14521 aagtcaatta acacggagag actaacgtga agttcaaagt tatcgccctg gcggcattaa
    14581 tgggtattag cgggatggca gcgcaggcta acgaattgcc ggatggaccg catattgtca
    14641 cctccggtac ggcaagcgtg gatgcggtgc cagacattgc cactcttgcg attgaagtca
    14701 acgtggccgc gaaggatgcc gctactgcca agaaacaggc ggatgagcgc gtcgcacaat
    14761 acatttcctt ccttgaactc aatcagatcg cgaaaaaaga tatcagctca gcgaatttac
    14821 gcacccagcc agattacgac tatcaggatg gtaaaagtat cctgaaaggc taccgcgctg
    14881 tgagaacggt ggaagtcacg ctccgtcagt tagacaaact gaattccttg ctggatggcg
    14941 cgctgaaggc gggtcttaac gaaattcgtt ctgtgtcgct gggcgtggcg cagccggatg
    15001 cctataaaga caaagcgcgt aaggcagcga ttgataacgc gattcatcag gcgcaggaac
    15061 tggcgaacgg ctttcatcgt aaactggggc cggtatatag cgtgcgctac catgtttcca
    15121 actatcagcc cagcccaatg gtgcggatga tgaaagccga tgccgcgccg gtgtctgccc
    15181 aggaaactta cgagcaggcc gctattcagt ttgatgatca ggtcgatgtg gtcttccagt
    15241 tagaacctgt ggatcaacaa cccgctaaaa cacctgcagc acaataatac ttgtagcccg
    15301 acgtattcgt cgggctaatg cctttacccg atattgcctc ctttttctgt aattttctgt
    15361 aaaaccatga cgtaatcacc tatctttgct cgcggtttca tcaaggatag gctgactgaa
    15421 acggttaagc ggacaaggat gcggggaggg gggatggaca tttttatttc gaaaaagatg
    15481 cgcaatttca ttttattagc gcaaaccaat aatattgccc gggcggcaga aaaaatccat
    15541 atgacggctt cgccatttgg taaaagcatt gccgcgctgg aagaacaaat tggctatacg
    15601 ctatttaccc gcaaagataa taatatcagc ctcaataaag ccggtcagga actgtatcaa
    15661 aaactgttcc cggtttatca acgactttct gcgattgata atgaaatcca taactcaggg
    15721 cgtcgttcac gagaaattgt gattggcata gataatactt atccgacaat tattttcgat
    15781 cagttgatta gtctcggaga taagtatgaa ggcgtgaccg cccagccagt tgagttcagc
    15841 gaaaacggcg ttatcgataa tctttttgac cgtcagttag attttattat ctccccacag
    15901 catgtatctg cacgagttca ggaactggaa aacctgacta tcagtgaatt accaccgctg
    15961 cgattgggct ttctcgtttc tcgccgttat gaagagcgtc aagagcaaga gctgcttcag
    16021 gagctgccct ggctacaaat gcgctttcaa aaccgggcta acttcgaagc aatgattgat
    16081 gcgaatatgc gaccttgtgg gatcaatccc accattattt atcgtcccta cagttttatg
    16141 gccaaaatca gcgccgtgga gcgcggacat tttctgacgg tgatcccgca ttttgcctgg
    16201 cgactggtga atccggcaac gctgaaatat tttgatgcgc cgcacaggcc gatgtatatg
    16261 caggaatatc tttattcaat cagaaatcat cgttataccg ccacgatgct tcagcatatt
    16321 gctgaagatc gtgacgggac gagtcattaa cccagcatcg agccggttgc aattaaatta
    16381 cggtgtaagt cgaagacgtg gctaagatcg tggtgaatat gtccgccagg cgcattttcc
    16441 agatagcgat gcagataatc ccgatacata ggatgtgcac aattatcaat gatagtgcgg
    16501 gcgcgttgaa gcggggaaag accgcgcaga tcggcgatcc cttgttcggt gatgatcact
    16561 ttgacgctgt gttcgctgtg atcaacatgg ctgcacattg gcacgacggt tgagatcttg
    16621 ccttctttag caatcgacgg ggccataaag atcgacagat acgcgttgcg ttcaaaatca
    16681 ccgctgccgc cgatgccgtt catcagatcg accccggcta cgtgcgttga gttggcatgc
    16741 ccgtaaatat caaactccag gccgacgttc agagcgatga cgcccagacg acggatgatt
    16801 tccgggttat tggaaatctc ctgcggacgc aacacaatgc ggctggcaaa gtaatccata
    16861 ttgtcgtaaa tcttgcgcag ggaatcggcc gagattgtca ggctggaggc gctggccccg
    16921 ctgattttgc cggtttccag taaatgcacc accgattcct gtagcacttc cgaatacatc
    16981 ataaacggag gaatttccgg gttttccccc agacgcgcca ttaccgcatt attgatattg
    17041 cccacgccac tttgcagcgg cagaaattcc ggcggaatac gcccatgcgc catttcctgc
    17101 aataagaacg tgaccacgtt atcggcaatc tgctggcaca tgggattttg cttatccagc
    17161 atattaccgg cgtcgggcaa gttggtttcc acgacggcga caatcttttt cggatcgatt
    17221 tgcacatagc gggtaccgac gcgatccatt gcatggaaga tcgacacgct attgcgccgc
    17281 ggtggcgcgc caggaatcac aatatccgcc agttctgcaa cgcgcggatc gtgatagtga
    17341 ttaagttcaa tgatcacttt cttcgcccgc agaagccatg tcggcgcatt accgatcccg
    17401 ctggttaacc agactcgacc atccggtgcc agtgccgatg cttcgatgac ggcaacatca
    17461 atgtcgccga agaaaccgta attgaccatt tgcgccactt cgctcaaatg caggtcaacg
    17521 aaactcaccg cgccctgatt gatcttttta cgtaaaccgg acgatgtttg atatggcgca
    17581 cgccaggaaa cagcatcggc gtcagaaagt acatcgtcag cggcggcgct gattgacgcc
    17641 cccgtcagca ggcgaatttg atacggcttt ttggcctcat gctgttcgtt agctctgcgg
    17701 gcaatcgcgg tgggtagggc tttcggcgaa cccgccgggg taaagccgct aaatgccacc
    17761 atgtcgttat gctggataat ttctgccgct tcatcggcgg tcatccttgt ccactgagtt
    17821 tccattttta ctccttcgtt cgcagggatt aatgaccaac gaaattaggt ttacgttttt
    17881 cgaggaaagc gttcattcct tcctggtaat cttcgctgtc ataaaccgcg cgtcgcatcc
    17941 cctgaatacg ttcaaattca tcggagttca tggtgtgcgc ttcgcccagt acacgcagct
    18001 cttctttgat aacagcaatg gctaacggcg ctttctcaga gatgtggtgc gccatttgta
    18061 aggtgaaatc ttccagttct tccacttcca caacatggtt gaggatgccg acagccaacg
    18121 cgcgctgggc ggtgattggc gaagcggtaa aaatcagctc tttgacaatg tggaagcccg
    18181 cgtcgcgggt caggttgtga atgccgacca ggttatacgg gacgccgagg tttacaggcg
    18241 tcattgagaa ggttgaggta ctggcggcga taatcagatc ggaactcatg atcatttcaa
    18301 atgcgccacc ccaaacacta ccttccacca tcgaaatgat cggtttcggg aatttttgga
    18361 tcatgcgggt gatttgacgc aatggatcat cataggagag cggatcgcga ccgccagacg
    18421 gcagttcgtg aatatcgtgg cctgcggaga agactttgga tccactcggc gcgcgcaaaa
    18481 tgatacagcg aatttccggc cggttgagat cgcttaatgc ctgcataaga tcatcaataa
    18541 agactttact taaggcatta agtttgcggc catagttaaa ctcaatgacc gccactttgt
    18601 tgatagtgac aacgttaaca tactgataag acataaaaat tcctttaatc aaaatattgc
    18661 gtctggataa attcactgag ctgccgcagg ccggtgcgcg gtgagagcgt attgtttttg
    18721 accgctaaaa gcgtctggcg gtaatagcga tcgaaatctt cattcgcgaa caggtgatgc
    18781 agtacttctt cttcggtctg cttacgcagc cattccaccg attgttgttg ccgcacttgt
    18841 tgtaaacgac cactggcagt tagcgcggtt ttgaagtcga tgatggcgtg ccagatctca
    18901 tcgattccac gtttttccag tgcgctacaa gtcagaaccc gtggctgcca ttcgtcgtat
    18961 ttacgtcgca gaatatgcag ggcactctcg tacatatgcc gggcaatggc gacattggta
    19021 tggttatcgc catcgtcttt gttgataacg agcagatcag ccacttccat cagccctttt
    19081 ttaatgccct gcagatcatc gccgccaccg gcaatttgca acgagataaa acagtccacc
    19141 atgcgggcga cttccgtttc cgactgcccg acgccaaccg tttcgacaat cactacgtca
    19201 taacccgccg cttcgcataa cagcattaat tcccgcgctc gctgactggc accgcccaga
    19261 tgaccggagg atggtaccgg gcgaataaac gccgcttcgg cacgcgccag gtcattcatg
    19321 cgggttttat ccccgagaat gctaccgcca gtgaccgggc tgctgggatc gaccgcaata
    19381 accgcgacct ttaatccctc acgaatcaac aacatgccaa aggcttcaag aaaggtactt
    19441 ttccccgcgc cgggtgtgcc ggtaacgccc agtcgcaggg cgttaccgca gtacggcata
    19501 atggcatcaa gcagttgcgt acttagcgcc tgatgacgcg ggtgacggct ttccaccagc
    19561 gtcatggcct gggcgagtgt ggcacgctca ccctgacgta agcggcgaat actttctgcc
    19621 agcgtggctt cattaatcat gatgctggct tatcagattc agtacgtcgc gcacactctc
    19681 gagcataggt gtacctggac cataaatcgc cgccacgccg cgctcttgca ggaaggcgta
    19741 atcctgcggt ggaatgacgc caccggcgac tacgcagata tcttcgcgtc cccatttttt
    19801 cagcgcttcg accagttccg ggatcagcgt tttatgaccg gcagccagtg aggatgcgcc
    19861 cactacgtga acgtcgtttt ctacggccag gcgggcgatc tcttcaggtg tagagaacat
    19921 cgggcttaaa tctacgtcga aaccgagatc ggaataggcg ctggcgatca ctttcgcgcc
    19981 gcgatcgtgt ccatcctggc ccatcttagc gatcagaatg cgcgggcgac gaccattgtc
    20041 ggcaaggaac tgctccgttt gcgcaacaat ggcatcgaac tcggaggccg atttctcaga
    20101 ctgatgatag ctttgcgcaa tcacgccggt aacacactgg cttggcacca gataacggtc
    20161 gaaagcgact tccagcgcat cggaaatttc acccagggtg gcgcgaacgc gagcggcatt
    20221 aacagcggca gccagcaggt tttcgttatg ctgtgcggcg tgagtcaggg cgttcaacgc
    20281 ggcggttacg gcggcatcat cacgggtggc gcgaatgcgt tccagcgaag caatttgctc
    20341 gttacgcacc atcacgttgt cgatctcaag tacatcggtt tcgtcttcgt gatccagttt
    20401 gtacttgttg acaccaacga tgacacgctt gccctggtcg atcagcgact gttcgcgcgc
    20461 tgaggcctct tcgatcattc gttttggcag acctgcttcg atcgctttcg ccatgccacc
    20521 ggcttcgtcg atctattgga taatagctct ggcttgtttg acgatttgat cggtcagcga
    20581 ctcaatgtaa taggatccgg ccagtggatc gacggtgcgg cagagttctg attcttcctg
    20641 gatgatgatc tgggtgttgc gggcaatgcg tgctgagaaa tcggtaggca aaccaagcgc
    20701 ttcgtcaaag gcgttggtat gcagtgactg agtaccgccc agcgtcgcag ccagcgcttc
    20761 aatggtggtg cggataacgt tgttatacgg atcctgttca gtcaggctcc agcctgaggt
    20821 ctggcagtgg gtacgcagcg ccagtgattt cgggtcctgt gcgccaaatc cactgacctc
    20881 ttcgctccat aaataacgtg ccgcacgcaa catggcgacg ttcataaaca gatccatgcc
    20941 gatgccgaag aagaacgaca ggcgaggagc gaagtcatca attttcagtc cggcagagat
    21001 tgctgctttg atgtactcaa tcccatcagc gagcgtaaat gctacctgct gcacgcagtt
    21061 ggcacccgct tcacccatgt ggtaaccgct gatactgatg gtattaaatc gcggcatgtt
    21121 gccggaacac caggcgatga tgtcggcgat aatgcgcatt gacggttttg gcgggtaaat
    21181 ataggtgttg cggcagaggt actctttgag aatatcgttt tgaatggtgc cggtcagttt
    21241 atcaggtgta acaccttgct cttctgcggc gacgatataa aacgccagta ctggtagcac
    21301 tgcgccattc atggtcatcg aaaccgacat tttatccagc gggatctggt cgaacaggac
    21361 tttcatatct tccacggtgt cgatagcgac gcccgctttg ccgacgtcac ccgccacgcg
    21421 tgggttatcg gagtcgtagc cacggtgggt ggcaaggtca aacgcaacgg aaagaccttt
    21481 ttgcccggcg gccaggttac ggcgataaaa agcgttggac tcttttgctg ttgaaaaacc
    21541 agcatactga cggatggtcc acggttgggc ggtatacata gtggcacgcg ggccacgaac
    21601 gtagggcggc aaaccaggaa gggtacctgt cacctccaga ttatcgagat cggcttcggt
    21661 atacagcggc ttgatggcga tcccttccgc ggtttgctga accagcgagt cgacagtttt
    21721 ctcccgacgg ctcaattcct tgttggcaag ctgttgccac tcctgctcgt tagacattat
    21781 ttgctccgta aaacgcaatc aaaatgaggg tatttgcgct tacggtgagc tttttacgtg
    21841 ggtttgtcgc cagcaaaaaa aatgcattca gacgtcgttt tttgccgcct gagatttgtc
    21901 tattttgtga tgtgattcaa ggaatacaaa aagaagtgag gacaacgcct gatatgtgct
    21961 atcaggcatt atttgatgga ttaatcctga cgaaggactt tgtgaccata atcgagtaac
    22021 gcatcagtga ctttacgcat catgcggctt tcaggagcaa agcggtgcca gtagagcatc
    22081 cgtcgttgaa atagcccagg cgttaagtca atcagttcac cgctggccag ctctttctcg
    22141 atttgcaggt gcgggatcat acagcaggtg gtgccctggc gagcaagttg tacgaacgct
    22201 tctgaagaat taacgatatg gcagggcacg ctgcctggag gcagatcgaa gttttgctgc
    22261 aaaaaggcct ggtgcatatc gtcaagatgg tcaaacgcga ccactggcgc tttcagtaat
    22321 gccgaacgcg ttacgccgtt agggaaatat ttttcggcaa agggttttga gctgacgaac
    22381 agatagtcga gcgcaccaag tttatcgaca agacaactcg gcagcgcctg atgttgaata
    22441 ctcaccgcgc cgaccacttc gccgcggcgc agacgttcct gagtgcgggt ttcatcttct
    22501 acctgcaagt tgaggcggat aggcgaatca gccaacacag gagccagtgc aggaagcaac
    22561 cacgtcgcca gactgtcggc gttgaccgcc agtgaaagca gcagcggagt cgaaccggtt
    22621 tgttcatcgc ccagccactc ttcttccagc aactccacct ggcgcagcag tgccagcagt
    22681 ttttgccctt gttccgtcgg gcgcggcggt acggtacgca ccaacagcgg ctgcccgaac
    22741 atattttcca gttgcttaat gcgctgtgag acggctgatt gtgtaatgca cagcttttgt
    22801 gccgcgcgct caaatcctcg ttcacgtatc accgcatcca gtgcctgtaa tgttctgtag
    22861 tccgggcgtt tcattgctgt tgtctgctcc tggtaaatgc tttttcctgc actatgacac
    22921 aattttgtgt caggttgcat ataccgaagc cgtcggcggg taacatttgt attcgtggtt
    22981 aaacgcgcag cttaaaagat cagggatttg cgctaatgca tttcgctcag cgagttcgcg
    23041 ccctggttgt gttgaatggc gtggcgttat tgcctctgtt cgcaccgtgg agcggcatac
    23101 ccagaccgtt gtatgcttta tttgcggggc gaaaggggat gcccgccatt gcgcgatatt
    23161 ttatggatga gttaaccacg cggcttgcca acggggtctg aatcgctttt tttgtatata
    23221 atgcgtgtga aatttcatac cacaggcgaa acgatcatga cgcaggatga attgaaaaaa
    23281 gcagtaggat gggcggcact tcagtatgtt cagcccggca ccattgttgg tgtaggtaca
    23341 ggttccaccg ccgcacactt tattgacgcg ctcggtacaa tgaaaggcca gattgaaggg
    23401 gccgtttcca gttcagatgc ttccactgaa aaactgaaaa gcctcggcat tcacgttttt
    23461 gatctcaacg aagtcgacag ccttggcatc tacgttgatg gcgcagatga aatcaacggc
    23521 cacatgcaaa tgatcaaagg cggcggcgcg gcgctgaccc gtgaaaaaat cattgcttcg
    23581 gttgcagaaa aatttatctg tattgcagac gcttccaagc aggttgatat tctgggtaaa
    23641 ttcccgctgc cagtagaagt tatcccgatg gcacgtagtg cagtggcgcg ccagctggtg
    23701 aaactgggcg gtcgtccgga ataccgtcag ggcgtggtga ccgataatgg caacgtgatc
    23761 ctcgacgtcc acggcatgga aatccttgac ccgatagcga tggaaaacgc cataaatgcg
    23821 attcctggcg tggtgactgt tggcttgttt gctaaccgtg gcgcggacgt tgcgctgatt
    23881 ggcacacctg acggtgtcaa aaccattgtg aaatgatctg acgggggaac ctcccccgtt
    23941 aaaaaaattc tcttcattaa atttggtgac atgtgtcacg tttttacctg gctattgtcg
    24001 attgctctaa ataaatcttc taaaccagca tattcatcca agaattacct ttgcgtgata
    24061 tttcctcaac atcgcgacgc aaacgttcat attgccgcaa tattattttt tgatatgttg
    24121 aaaggcggac gcaaatccgc acacaacatt tcaaaagaca ggattgggta aatggcaaag
    24181 gtatcgctgg agaaagacaa gattaagttt ctgctggtag aaggcgtgca ccaaaaggcg
    24241 ctggaaagcc ttcgtgcagc tggttacacc aacatcgaat ttcacaaagg cgcgctggat
    24301 gatgaacaat taaaagaatc catccgcgat gcccacttca tcggcctgcg atcccgtacc
    24361 catctgactg aagacgtgat caacgccgca gaaaaactgg tcgctattgg ctgtttctgt
    24421 atcggaacaa accaggttga tctggatgcg gcggcaaagc gcgggatccc ggtatttaac
    24481 gcaccgttct caaatacgcg ctctgttgcg gagctggtga ttggcgaact gctgctgcta
    24541 ttgcgcggcg tgccggaagc caatgctaaa gcgcaccgtg gcgtgtggaa caaactggcg
    24601 gcgggttctt ttgaagcgcg cggcaaaaag ctgggtatca tcggctacgg tcatattggt
    24661 acgcaattgg gcattctggc tgaatcgctg ggaatgtatg tttactttta tgatattgaa
    24721 aataaactgc cgctgggcaa cgccactcag gtacagcatc tttctgacct gctgaatatg
    24781 agcgatgtgg tgagtctgca tgtaccagag aatccgtcca ccaaaaatat gatgggcgcg
    24841 aaagaaattt cactaatgaa gcccggctcg ctgctgatta atgcttcgcg cggtactgtg
    24901 gtggatattc cggcgctgtg tgatgcgctg gcgagcaaac atctggcggg ggcggcaatc
    24961 gacgtattcc cgacggaacc ggcgaccaat agcgatccat ttacctctcc gctgtgtgaa
    25021 ttcgacaacg tccttctgac gccacacatt ggcggttcga ctcaggaagc gcaggagaat
    25081 atcggcctgg aagttgcggg taaattgatc aagtattctg acaatggctc aacgctctct
    25141 gcggtgaact tcccggaagt ctcgctgcca ctgcatggtg gtcgtcgtct gatgcacatc
    25201 cacgaaaacc gtccgggcgt gctaactgcg ctgaacaaaa tcttcgccga gcagggcgtc
    25261 aacatcgccg cgcaatattt acaaacttcc gcccagatgg gttatgtagt tattgatatt
    25321 gaagctgacg aagacgttgc cgaaaaagcg ctgcaggcaa tgaaagctat tccgggtacc
    25381 attcgcgccc gtctgctgta ctaattcccc ttctctgaaa attaacgggc aggtcactga
    25441 cttgcccgtt tttttatccc ttctccacac cgttgccgcg ttgcgcattc tactttgcga
    25501 gtctgctcca gaaaataaat caaagtcaga tcaatgcgtt gtgtttattt aatggggatt
    25561 tacgatggcg ggacagcatg gggctgtaac ggtaaagccc tcaccgaagc gagggcttaa
    25621 aggagaaggg ttatgatgcg acttgtcatc atactgattg tactgttact gataagtttc
    25681 ggagcttatt aacagtcagt ctcaggggag gagtcatcct cccttaccct tactcactaa
    25741 attaggtcaa agaatcaacg atgtcaatca gggcgatgcg gggtgcatcg ccctgaaccg
    25801 aactttttga tagccacttc atcgcccacg tttactgccg taatagatcc tttttcccaa
    25861 aaattatctc tgttttgcat ggggtttttc gagcacgctc gaagaaaaga tttaaaggta
    25921 gatcaatgcg ttgtggttat ttaaagagag cttacgatag ctgggcagca tggggctgta
    25981 acggtaaagc cctcaccgaa gcgagggctt gaaggagaag ggttatgatg cgacttgcca
    26041 tcatactgat tgtactgtta ctcataagtt tcccagctta ttaacagtca gtcacagggg
    26101 aggagcaatc ctcccttacc cttactcact aaattaggtt aaagaatcaa cgatgtcaat
    26161 cagggcgatg caggtgtatc gcccttacca ctcccagact ttcgacggtg taaccaccgc
    26221 aggaagaggg atatcccact cttcaacggg gagtttttcc accaactgac aatcatgcgc
    26281 ataacccacc ggttgcgttt tatagtgctg ccagttttgt aaggtccgat cataaaaacc
    26341 accgcccatt cccaggcgct gaccgtactc atcaaaggcg accagcggtg tgatcagcac
    26401 gtctaatcgg gaaaggggta gcacgtcacg cacatccagt tttggctcat ggatcttcaa
    26461 cctgttcatc accagttcgc tttgcggatg gtaattcagg aacagcaaat taccggcact
    26521 aaagggatgc aaaactggaa gatatacgcg cttaccggcg cgccagagtt gttctattag
    26581 cggctgggtg tcgagttcac catcaaaaga gaggaataca gcgaccgtat gcgccatcac
    26641 caccggggga taagtcatca tccgggtagc ggcttgttga cccatttcct gctgttgttc
    26701 cggcgttaac gcacgacgac gttgccgaat cattttgcgg atttcttgtc gggataatgt
    26761 cagtgggagt tctggtagtt gcgtcatggc tggtgccaga taagaaggga atctccgaga
    26821 tgccgccgca ggctgtaacc cttgaaccct tggttcaagg tgaatgtgtc gtcgcagttt
    26881 taaggcttct cggacggacc gagcatgctc accaaccgcg gagcgccaca ttcttgtggt
    26941 atgaaatatc ggctcagggg actggcccgc ttgcgaacat ctcagagaaa ttttgtcttc
    27001 acggttactc taccacagta aaccgaaaag tgtcattcaa agttttggtt agttttttcg
    27061 gtgatgcgac cttgttcaag taacgcttgt tctatggtct gctgcagcat ccgaatacgc
    27121 tgttccatac ttgccgcgta gtcacgagtc ttcgcttttt cttgcgctaa ctcatagctg
    27181 atattcaatg cggcaatgaa gaccaactgt tcagtatttg tgactctagt gcgttctttc
    27241 agatcttgca accgttggtt cagatcgtcc gctgcctgat tcaacgcatc cctttggtca
    27301 ggcgggcagt tcacacgcag tgaacggcca aaaatttgga tatcgacggg ttgtgcagac
    27361 atgccacctt cctgctgatt gactgcgctg ccttcgtctt tagacccatg gtctgcgaag
    27421 gggcgacact atagctaccc tgatgagaag agacaagccc ttttctggtc caccagggcc
    27481 caaagtggta gcatatcatg aatattcctc cctttgacga cgaatgctta tgtctataca
    27541 gaacgaaatg cctggttaca acgaaatgaa ccagtatctg aaccaacaag ggacgggtct
    27601 gacccctgct gagatgcatg gtttaatcag tgggatgata tgtggcggta acgatgacag
    27661 ctcatggcta ccgctacttc acgacctgac gaacgaaggc atggctttcg gtcatgagct
    27721 ggcacaggca ctgcgtaaaa tgcactctgc caccagcgat gccctgcagg atgacggctt
    27781 cctttttcag ctttatctgc ctgatggcga tgatgtcagc gttttcgatc gggctgatgc
    27841 attggcaggt tgggtcaatc acttcctgct tggtcttggc gttacgcaac cgaagctgga
    27901 taaagtgacc ggcgaaaccg gtgaagctat cgacgatctg cgtaacattg cgcaactggg
    27961 ttacgacgaa gacgaagatc aggaagagct tgaaatgtcg cttgaagaga tcatcgaata
    28021 cgttcgtgtt gccgcgctgt tatgccacga cacctttact catccgcaac cgaccgcgcc
    28081 agaagtacaa aaaccgactc tacactaaaa acaaaaaacg taaggagagt gttatgagtg
    28141 agatatcccg gcaagagttt cagcgtcgcc gtcaggccct ggtggagcaa atgcaacccg
    28201 gcagcgccgc gctgattttt gctgcaccag aagtaacacg tagcgccgac agcgaatacc
    28261 cctatcgtca gaacagtgac ttctggtact tcaccggctt taacgaaccg gaagcggtgc
    28321 tggtgctgat taaaagcgat gacactcata accacagcgt tctgtttaac cgcgttcgcg
    28381 acctgacggc ggagatctgg tttggccgtc gtttaggtca ggatgccgcg ccagagaaac
    28441 tgggcgttga ccgcgcactg gcattcagcg aaattaatca gcaactttat caattactta
    28501 atggtctgga tgtggtttac cacgcccagg gcgaatatac ctatgctgat gaaatcgtga
    28561 acagcgcgct ggaaaagctg cgtaaaggtt cgcgacaaaa tctcaccgcg ccggcaacga
    28621 tgatcgactg gcgtcccatt gttcatgaaa tgcgcctgtt caaatcgcca gaagagattg
    28681 ccgtacttcg ccgcgcgggt gaaatcaccg ccctggcgca tacccgggcg atggaaaaat
    28741 gccgtccggg aatgttcgag taccacctgg aaggcgaaat tcaccacgaa tttaaccgcc
    28801 acggcgcgcg ctatccttcc tataacacca ttgtcggcag cggtgaaaac ggctgcattt
    28861 tgcactacac cgaaaacgag tgtgaactgc gtgacggtga cctggtgttg attgacgcgg
    28921 gttgtgaata caaaggttac gctggcgata ttacccgcac cttcccggtc aacggcaaat
    28981 tcactcaggc ccagcgtgaa atctacgaca ttgtgctgga gtctctcgaa accagcttgc
    29041 gcctgtatcg tccgggaact tccatgcagg aagtcaccgg tgaagtggtg cgcatcatgg
    29101 ttagcggcct ggtaaaactc ggcattctga aaggtgatgt tgatgaactg atcgctcaga
    29161 acgcccatcg tcctttcttt atgcatggcc ttagccactg gttagggcta gatgtccatg
    29221 acgttggcgt ttatggtcag gatcgctcgc gcattctgga accgggcatg gtactgaccg
    29281 tagagccagg gctgtatatt gcgccggatg cagaagtgcc agaacaatat cgcggtatcg
    29341 gcattcgtat tgaagacgac attgtgatta ccgaaaccgg taacgaaaac ctcaccgcca
    29401 gcgtggtgaa aaagccggaa gaaatcgaag cgttgatggc agctgcgaga aagcaatgag
    29461 cgtaatcatc gtcggtggcg gcatggcggg cgcgacgctg gcgctggcta tttcccggtt
    29521 aagtcacggg gcgctgccgg tacatttgat tgaagcgacc gcgccagagt cacatgctca
    29581 tccgggcttt gatggacgag ctattgcgct agcggcgggg acctgtcagc aactggcgcg
    29641 catcggcgtc tggcaatctc tggctgattg cgcaaccgcc atcactaccg tgcatgtcag
    29701 cgatcgtggt catgctggat tcgtcaccct cgccgcagaa gattaccaac tggcggcgct
    29761 ggggcaggtt gtcgaattgc ataatgtcgg gcaacggctg tttgcattgc tgcgaaaagc
    29821 acctggcgta acgctgcatt gccctgatcg cgtagctaac gttgcccgta ctcagagtca
    29881 cgttgaagtg acgctggaga gcggcgagat gctgacgggc cgcgtgctga tagccgctga
    29941 tggcacccat tcagcgttag ccaccgcgtg cggcgttgat tggcagcagg agccttacga
    30001 acaactggcc gtgattgcca acgttgcgac ttccgttgcg cacgaaggcc gcgcttttga
    30061 acgcttcacg caacatggcc cgctggcgat gttgccgatg tctgacggac gctgttcgct
    30121 ggtctggtgt catccactgg aacggcgcga agaggtgctg tcgtggagcg acgagaagtt
    30181 ttgccgtgaa ctccagtcgg cctttggctg gcgacttgga aaaattaccc acgccggtaa
    30241 acgcagtgct tatccgctgg cgttaacccg cgccgccaga gctattaccc atcgtaccgt
    30301 gctggtgggc aatgcggcgc aaactctgca cccgattgcc gggcaagggt ttaacctcgg
    30361 tatgcgagat gtcatgagcc ttgcggaaac cctaactcag gcgcatgagc gcggagaaga
    30421 catcggtgat tacggcatat tgtgccgcta tcaacagcgt cgacagagcg atcgcgaagc
    30481 aaccatcggc gtcacggaca gcctggtaca tctttttgcc aaccgttggg caccgctggt
    30541 tgtcgggcgc aacatcggac tgatgacgat ggaattattc accccggcac gcgatgtgct
    30601 ggcgcagcgc accctcggtt gggtggcgcg ttgaaggcgg ttttaaggaa taaacaatgc
    30661 aaagtgttga tgtagccatt gttggtggcg gcatggtggg gctggcggtt gcctgcggct
    30721 tacaggggag cggcttacgc gttgccgtac tggagcagcg cgtaccggaa cctctggcgg
    30781 cggatgcacc accacaactg cgcgtttcgg ctatcaatgc cgccagcgaa aaattactca
    30841 cccgtcttgg cgtctggcag gacattctct ctcgtagggc cagttgttat cacggtatgg
    30901 aagtgtggga caaagacagt tttggtcaca tttcgtttga cgatcaaagc atgggctata
    30961 gccatcttgg gcatatcgtt gaaaactcag tgattcacta cgcgttgtgg aacaaagcgc
    31021 agcagtcgtc agatatcact ctgttggccc ccgcagaatt acagcaggtc gcctggggag
    31081 aaaatgaaac tttcctgacg ctgaaagatg gcagcatgtt aacggcgcgt ctggtgattg
    31141 gcgcggacgg tgctaattcc tggttgcgca acaaagcaga tattccgctg actttctggg
    31201 attatcagca tcacgcgctg gtagcgacca ttcgcacgga agaaccgcat gatgcggtgg
    31261 cgcggcaggt tttccatggc gaaggcattc tggccttttt accgcttagc gatccgcatc
    31321 tttgctcaat tgtctggtca ctgtcgccag aggaagcgca gcggatgcag caggcaagtg
    31381 aagatgaatt taatcgcgcg ttaaatatcg cttttgataa tcgcctgggc ttatgcaagg
    31441 ttgagagcgc gcgtcaggtg ttcccactga cggggcgtta tgcgcgccag tttgccgcgc
    31501 accgtctggt gctggtgggc gacgccgcgc ataccattca cccgctggcg gggcagggag
    31561 taaatctcgg ctttatggat gctgcagagc tgattgctga actgaaacgg ttgcatcgtc
    31621 aggggaaaga catcgggcag tacatttacc tgcgccggta tgagcgtagc cgcaagcaca
    31681 gtgcggcgct gatgctggct ggtatgcagg gattccgcga tctgttttcc ggtaccaatc
    31741 cggcgaaaaa actgctgcgt gatattggtt tgaaactggc cgacacgctt cctggcgtta
    31801 aaccacaact tatccgccag gcaatgggat taaacgattt gcctgaatgg ctgcgttaaa
    31861 aatttctctt ctgctgttta tttgataccc atcacacttt catctcccgg ttttttcgcc
    31921 gggagatttt cctcatttga aataaactaa ttt
//
LOCUS       DAZFQD010000048        31680 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.048, whole genome shotgun sequence.
ACCESSION   DAZFQD010000048 DAZFQD010000000
VERSION     DAZFQD010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31680
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.048"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(95..1759)
                     /gene="glnS"
                     /locus_tag="ACHKTW_002251"
     CDS             complement(95..1759)
                     /gene="glnS"
                     /locus_tag="ACHKTW_002251"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HFO6463270.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHTTVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVESIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(1962..3908)
                     /gene="nagE"
                     /locus_tag="ACHKTW_002252"
     CDS             complement(1962..3908)
                     /gene="nagE"
                     /locus_tag="ACHKTW_002252"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HFO6463271.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVVFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLNQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDTMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLIIKAQGHVVAGQTPLYEIKK"
     gene            4241..5041
                     /gene="nagB"
                     /locus_tag="ACHKTW_002253"
     CDS             4241..5041
                     /gene="nagB"
                     /locus_tag="ACHKTW_002253"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFO6463272.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMILVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            5101..6249
                     /gene="nagA"
                     /locus_tag="ACHKTW_002254"
     CDS             5101..6249
                     /gene="nagA"
                     /locus_tag="ACHKTW_002254"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFO6463273.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLAKHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIGVEKRLGTLAAGKVANLTAFTPDFKITKTIVNGNEVVTQ"
     gene            6258..7478
                     /gene="nagC"
                     /locus_tag="ACHKTW_002255"
                     /gene_synonym="nagR"
     CDS             6258..7478
                     /gene="nagC"
                     /locus_tag="ACHKTW_002255"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HFO6463274.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            7526..8278
                     /gene="nagD"
                     /locus_tag="ACHKTW_002256"
     CDS             7526..8278
                     /gene="nagD"
                     /locus_tag="ACHKTW_002256"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
                     /protein_id="HFO6463275.1"
                     /translation="MTIKNVICDIDGVLMHDNVAVPGAAEFLHGIMDKGLPLVLLTNY
                     PSQTGQDLANRFATAGVDVPDSVFYTSAMATADFLRRQEGKKAYVVGEGALIHELYKA
                     GFTITDVNPDFVIVGETRSYNWDMMHKAAYFVANGARFIATNPDTHGRGFYPACGALC
                     AGIEKISGRKPFYVGKPSPWIIRAALNKMQAHSEETVIVGDNLRTDILAGFQAGLETI
                     LVLSGVSSLDDIDSMPFRPSWIYPSVAEIDVI"
     gene            8535..10199
                     /gene="asnB"
                     /locus_tag="ACHKTW_002257"
     CDS             8535..10199
                     /gene="asnB"
                     /locus_tag="ACHKTW_002257"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HFO6463276.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKTHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKSLVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLL
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            10580..10656
                     /locus_tag="ACHKTW_002258"
     tRNA            10580..10656
                     /locus_tag="ACHKTW_002258"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10614..10616,aa:Met,seq:cat)
     gene            10665..10749
                     /locus_tag="ACHKTW_002259"
     tRNA            10665..10749
                     /locus_tag="ACHKTW_002259"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10699..10701,aa:Leu,seq:tag)
     gene            10773..10847
                     /locus_tag="ACHKTW_002260"
     tRNA            10773..10847
                     /locus_tag="ACHKTW_002260"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10805..10807,aa:Gln,seq:ttg)
     gene            10882..10956
                     /locus_tag="ACHKTW_002261"
     tRNA            10882..10956
                     /locus_tag="ACHKTW_002261"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10914..10916,aa:Gln,seq:ttg)
     gene            10972..11048
                     /locus_tag="ACHKTW_002262"
     tRNA            10972..11048
                     /locus_tag="ACHKTW_002262"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11006..11008,aa:Met,seq:cat)
     gene            11097..11171
                     /locus_tag="ACHKTW_002263"
     tRNA            11097..11171
                     /locus_tag="ACHKTW_002263"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11129..11131,aa:Gln,seq:ctg)
     gene            complement(11325..12500)
                     /gene="ubiF"
                     /locus_tag="ACHKTW_002264"
     CDS             complement(11325..12500)
                     /gene="ubiF"
                     /locus_tag="ACHKTW_002264"
                     /EC_number="1.14.99.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-demethoxyubiquinol 3-hydroxylase"
                     /protein_id="HFO6463277.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVMFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPGSLIALHRDNDLQELELKGGEVIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPVRIRQLQNMNMAQLQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
     gene            12646..14070
                     /gene="miaB"
                     /locus_tag="ACHKTW_002265"
     CDS             12646..14070
                     /gene="miaB"
                     /locus_tag="ACHKTW_002265"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HFO6463278.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRV
                     AETPESVIARTRKENDLGVGYYQP"
     gene            14223..15263
                     /locus_tag="ACHKTW_002266"
     CDS             14223..15263
                     /locus_tag="ACHKTW_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFO6463279.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            15260..15727
                     /gene="ybeY"
                     /locus_tag="ACHKTW_002267"
     CDS             15260..15727
                     /gene="ybeY"
                     /locus_tag="ACHKTW_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFO6463280.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            15817..16695
                     /gene="corC"
                     /locus_tag="ACHKTW_002268"
     CDS             15817..16695
                     /gene="corC"
                     /locus_tag="ACHKTW_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HFO6463281.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            16720..18258
                     /gene="lnt"
                     /locus_tag="ACHKTW_002269"
     CDS             16720..18258
                     /gene="lnt"
                     /locus_tag="ACHKTW_002269"
                     /EC_number="2.3.1.269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HFO6463282.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIVSLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIAAPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPIMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDGDQLLNTLKIYYNATAPLLGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSVNGIELTAAICYEIIL
                     GEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFTREALTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     ARKR"
     gene            complement(18336..20213)
                     /locus_tag="ACHKTW_002270"
     CDS             complement(18336..20213)
                     /locus_tag="ACHKTW_002270"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HFO6463283.1"
                     /translation="MSASSVKPLNVQLPAITLILFALCIGIFCYLAQWMSYEEVDQSA
                     LIHLGANVAPLTLSGEPWRLLSSIFLHSSVSHLLMNMFAFLVVGGVAEQILGKWRLLI
                     TWLFSGVFGGLISACYALRESEQIVISVGASGAILGIAGAAIATQFASGTGTYHKNQR
                     RVFPLLGMVALTLLYGARQTGIDNACHIGGLIAGGALGWLSARLSGQNRLVTEGGIIV
                     AGSLLMTGAIWLAQQQMDESVLQVRQSLREAFYPQEIEQERRQKKQQLAEERNALRET
                     LSAPVSREQASGDLLAEIADIHDMAISRDGNMLYAAIENTNSIVVFDLGQKKILHTFT
                     APIAKEKSVKHCGGCKDQGVRSLALSPDEKLIYATSFEANALSVINVATGEIIQSITT
                     GAHPDSLILSRDGTKAWVMNRTSNSVSAIDLVTYQHVADIPLEKYDGTGTSNKPCAWV
                     MALSPDEKILLIPGMVRGDIVRINTITHQKESYPASDARGTISAMRFRPENGDVIFAD
                     SQGISRISVGDQQASIMTQWCSRSVYSVEGISPDGQYLALVSYGLQGYVILLNINAGQ
                     IIGVYPASYVNHLRFSADGRKIFVMAKNGLIQLDRTRSLDPQAIIRHPQYGNVACIPE
                     P"
     gene            20668..21576
                     /gene="gltI"
                     /locus_tag="ACHKTW_002271"
     CDS             20668..21576
                     /gene="gltI"
                     /locus_tag="ACHKTW_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HFO6463284.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPAAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKDKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWEIVGKPQSQEAYGCMLRKDDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            21746..22486
                     /gene="gltJ"
                     /locus_tag="ACHKTW_002272"
     CDS             21746..22486
                     /gene="gltJ"
                     /locus_tag="ACHKTW_002272"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HFO6463285.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWIWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQAYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAVIMLVMTLVERKVRLPGNMGGK"
     gene            22486..23160
                     /gene="gltK"
                     /locus_tag="ACHKTW_002273"
     CDS             22486..23160
                     /gene="gltK"
                     /locus_tag="ACHKTW_002273"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HFO6463286.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVAWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            23160..23885
                     /gene="gltL"
                     /locus_tag="ACHKTW_002274"
     CDS             23160..23885
                     /gene="gltL"
                     /locus_tag="ACHKTW_002274"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP-binding
                     protein GltL"
                     /protein_id="HFO6463287.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMDPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            24003..24938
                     /gene="rihA"
                     /locus_tag="ACHKTW_002275"
     CDS             24003..24938
                     /gene="rihA"
                     /locus_tag="ACHKTW_002275"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050263 - ribosylpyrimidine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HFO6463288.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPTFAPQKC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYSQGMTVV
                     DYYYLTGNKPNATVMVDVDRQGFVDLLADRLKFYA"
     gene            25022..26692
                     /gene="hscC"
                     /locus_tag="ACHKTW_002276"
     CDS             25022..26692
                     /gene="hscC"
                     /locus_tag="ACHKTW_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
                     /protein_id="HFO6463289.1"
                     /translation="MDNAELAIGIDLGTTNSLIAVWKDGAAQLIPNKFGEYLTPSIIS
                     MDENNHILVGKPAVSRRTSHPDKTAALFKRAMGTNTNWRLGSDTFNAPELSSLVLRSL
                     KEDAEEFLQRPIKDVVISVPAYFSDEQRKHTRLAAELAGLNAVRLINEPTAAAMAYGL
                     HTQQNTRSLVFDLGGGTFDVTVLEYATPVIEVHASAGDNFLGGEDFTHMLVDDVLKRA
                     DVARTTLNESELAALYACVEAAKCSNQSPLHIRWQYQDETRECEFYENELEDLWLPLL
                     NRLRVPIEQALRDARLKPSQIDSLVLVGGASQMPLVQRIAVRLFGKLPYQSYDPSTIV
                     ALGAAIQAACRLRSEDIEEVILTDICPYSLGVEVNRQGVSGIFSPIIERNTTVPVSRV
                     ETYSTMHPEQDSITVNVYQGENHKVKNNILVESFDVPLKKTGAYQSIDIRFSYDINGL
                     LEVDVLLEDGSVKSRVINHSPVTLSAQQIEESRTRLSALKIYPRDMLINRTFKAKLEE
                     LWARALGDEREEIGRVITDFDAALQSNDMARVDEVRRRASVYLAIETS"
     gene            complement(26752..28203)
                     /gene="djlC"
                     /locus_tag="ACHKTW_002277"
     CDS             complement(26752..28203)
                     /gene="djlC"
                     /locus_tag="ACHKTW_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
                     /protein_id="HFO6463290.1"
                     /translation="MKTCWQILEIESTTQIDIIRQAYLARLPLCHPETDPQGFKALRQ
                     AYEEALRLAVNPVEEADDEEKDAAAEHEILRAFRTLLDSESDRFQPSAWQKFIQQLNT
                     WNMEDVDQLRWPLCAIAIEARYLSLNCASLLAERLNWHSFNDSEGMDEEEREAFLEAI
                     QAGDCFDFLSLLEYPVALQNQTVEYYFALERCCRYHPDYVTAFLAMEGPWFIPDDAKL
                     HRKLLRWYSSVQTGMAELIPVAKQWQMEEPESEDARYYLCAQRLYCGEGESLLADLCA
                     YRESYPSTQADNLLLQWSKRHCPDYFALLVMVIEARSMVDAQGQPLKYVPGESARTRL
                     LWAEILHSGKLSPLGQSFIESLFFKRKAWAWWKSRVGSETEEDSPLLDLYRVAEQVVL
                     EAFPKQEMLARLNTRLEGGDAHPLEAIITRMLLTKVKLEPEDEDVDEPTPENHEEKND
                     EGEKPQSITSIIKISLTVLVIGYALGKIAMLFS"
     gene            complement(28200..28907)
                     /locus_tag="ACHKTW_002278"
     CDS             complement(28200..28907)
                     /locus_tag="ACHKTW_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFO6463291.1"
                     /translation="MNKEEQYLLFALSAPMEILNQGCKPAHDSPKMYTGIKEFDLSSS
                     WGINNRDDLIQTIYQMTDDGHANDLAGLYLTWHRSSPEEWKALIAGGSERGLIYTQFV
                     AQTAMCCGEGGIKAWDYVRMGFLSRVGVLNNWLTEEESLWLQSRVYVRAHHYYHSWMH
                     YFSAYSLGRLYWQSSQCEDNTSLREALTLYKYDSAGSRMFEELAAGSDRFYATLPWQP
                     LTVQPECPVTLKDVSDL"
     gene            29008..29562
                     /locus_tag="ACHKTW_002279"
     CDS             29008..29562
                     /locus_tag="ACHKTW_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFO6463292.1"
                     /translation="MNKKLMYIFAIFIVAAITCISQPKKTTLRDKAMVNYAFDYLSSP
                     GSLPLTTAATELSAIHGHSTSQYRLGEFYLHGSDGKPLDYTQARYWYEQSAEQENPRA
                     QSKLGWIYLKGLGVKPDTRKAILWYKEAAEQGYAHAQYTLGLIYRNGTGINVNHYESQ
                     KWLKLAAKQHYKNAERLLAGLPAH"
     gene            complement(29572..30999)
                     /locus_tag="ACHKTW_002280"
     CDS             complement(29572..30999)
                     /locus_tag="ACHKTW_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HFO6463293.1"
                     /translation="MKNCWKILEIEETTDVDIIRRAYLALLPSFHPETDPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLPSAWQRFIQQLNY
                     CSMEDIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQTILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSAGRLDIPELLDYARSWREAEPDNEDARYYEYAQRVYCGEGESLLAELCD
                     YWREYPSTQADALMLLWCRQHRVDYYPLVVMMIEARDLVNDKGKPLLYVPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHKGRKPRISLTRDTEHPLWPLYLVAKQLVQASQPT
                     EESLMPIVSRLDAEDRCPLEALIIRRLLIQAANFTGQETVEPEPQPQPMPVDDGGLGC
                     LGVIKIIFYIFIFAGLIGKILHLFG"
     gene            complement(30996..31679)
                     /locus_tag="ACHKTW_002281"
     CDS             complement(30996..31679)
                     /locus_tag="ACHKTW_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFO6463294.1"
                     /translation="MFALSTPMEIRNECCLPSHSSPKMYLGTCFFDLSSSWGIDDRDD
                     LLRTIHRMIENGHAARLAGFYHRWFRYSPCEWRNYLAELNEQGQAYAQFVASTAECCG
                     EGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWIQSRIHLRALRYYRNWRQYFAGYTFG
                     RQYWHSPEDDNLQLLREFLARKEYDDSGNDMFYQLFASDNAYYPTLSWQPLAYYSACP
                     ETLKDMSDL"
BASE COUNT         7918 a         8164 c         7908 g         7690 t
ORIGIN      
        1 agaaaaaacg ccgcagagtg cggcgtttgg gattgtttgc tatttcttgc cggatgcgac
       61 gtaaatgcct cacccggcct ggtagaactt aaaattactc gcctactttc gcccaggtat
      121 cacgcagccc aacggtgcgg ttaaataccg gtttttccgc tgtggaatgg cggctgtcga
      181 ggcagaagta accttcacgc tcaaactgga atgctttacc cgcaaccgca tctttcagcg
      241 acggttcagc aaagccctgt ttgatcacca gcgattccgg gttaatcacc gacaggaaat
      301 catccgcagc accagggttc ggcacgctga acagacggtc gtacagacgg atttcaaccg
      361 gcagcgcatg tgccgcgctc acccagtgaa taacaccttt gactttacga ccatctgccg
      421 gatctttgct taaggtgtcg gcgtcataag tacagaagat ggtggtgata ttgccttcgg
      481 catctttctc cacgcgttct gccttaatga cgtaagcatt acgcagacgc acttctttac
      541 ccagcaccag acgtttgtac tgcttgttag cttcttcgcg gaaatcggcg cgatcgatcc
      601 aaatctcacc gctaaacggc acctggcggc tacccatttc cggtttgttc ggatggttcg
      661 gcatggtaac catttcgcct tcgccctgat agttttcgat aaccagtttc accggatcga
      721 taaccgccat tgcgcgcggc gcattttcgt tgagatcttc acggatgcag gattccagcg
      781 atgccatctc aatggtgttg tcctgcttgg tcacgccgat gcgtttgcag aactcacgaa
      841 tagaagcagc agtgtaacca cgacgacgca gaccggaaat ggtcggcata cgcgggtcat
      901 cccagccttc aacgtgcttg tcggtcacca gcaggttcag cttacgcttg gacatcacag
      961 tgtattccag attcaggcgc gagaattcgt actggcgcgg gtgaacagga atggtgatgt
     1021 tgtccagcac ccagtcgtac aggcgacggt tgtcctggaa ctcaagcgta cacagagagt
     1081 gcgtaatacc ttccagggca tcgctgatgc agtgggtgaa gtcgtacatc gggtagatgc
     1141 accacttgtt gccagtctgg tggtgttcag caaatttaat acggtacagc accggatcgc
     1201 gcatcacgat aaacggtgaa gccatgtcga ttttcgcacg caggcaggct ttaccttctt
     1261 caaaaccacc ggcacgcatt ttttcgaaca gcgccaggtt ctcttcaacg ctgcggtcgc
     1321 ggtacgggct gtttttaccc ggttgcgtca gggtgccgcg gtattcgcgg atctgttccg
     1381 gcgtcagttc atcaacgtac gccaggcctt tattgatcag ttcgatcgca taggcgtgga
     1441 gctgatcaaa ataatcggag gagtaacgga cgttaccaga ccagtgaaaa cctaaccact
     1501 ctacgtcgtt tttgatcgac tcaacatact cgatatcttc ttttaccggg ttagtgtcgt
     1561 cgaaacgcag gttgcactgg cctttatagt cctgggcgat cccgaagttc aggcagatag
     1621 atttcgcatg gccaatatgc agatagccat tcggctccgg cgggaaacgg gtgtgtactg
     1681 tggtgtgctt accactggcc agatcttcat cgatgatctg acggataaag ttagtcgggc
     1741 gggcttctgc ctcactcatc gtggattcct caaagcgtaa acaacgtata acggcgtatg
     1801 atcttataag tgggacaggc tgacaactgt tagtttttta aaattgataa tctaacaagg
     1861 attatgggga tgattgatga aaaaaaaagc ggcagagaag atctccaccg cttgtcattg
     1921 ttggatgcga cgctcaagcg tcgcatcagg cataaagcag attacttttt gatttcatac
     1981 agcggtgttt gacccgccac aacatggccc tgagctttaa tgatcaagcc actgaaatcg
     2041 tcgatattgc tgcaaaccac cgggctaatc atcgagcggg cgttagcgtt caggtaatcc
     2101 agatccattt ccagaatcgg ttgccctgcg cttacctgcg caccctcttc caccagacgt
     2161 ttaaagcctt taccttccag cgctacggtg tcgataccca tatggacgac gatctccgcg
     2221 cctttttcgg tttccaggca gaacgcgtgg ttggtgttga agattttcac gattgtccct
     2281 gcggctggtg atacgacgat tttatctgtc ggtttcaccg ccacaccgtc acccaccgct
     2341 ttgctggcga atgcttcgtc aggaacctga tccagtgcca cgacatcacc ggtaatcggc
     2401 gataccagct ccgcgataga taccgcgttt ggtacagcct gcggttttgc tacaggcgcg
     2461 gcagttgccg gagttgcttc agctgacgca gcggctaccg gaccacgggc aacgactttc
     2521 ttcatcgcat cgccgatgga ttctgctttc gcgccaacaa tcacctgaat agtctgtttg
     2581 ttcagtttca ctacaccaga agcacccaga cgtttacaca tcgtatcgtt gacgcgggca
     2641 gagtcagcca cggtcaggcg cagacgggtg atacaggcgt caattgcttt caggttgtca
     2701 gtgccgccaa ccgcagcaat atagttggtc gccagttgat tgaggccttc ttcagtgttg
     2761 ctgttggctt cttcagtaac gatctcgtct tctttatctt cacgacccgg cgttttcagg
     2821 ttgaacatgc ggataaccaa actgaacacc acgaagtaga tagcgaagaa gacaacaccc
     2881 ataaccagca gcatccagac gttctggctg gcggccggca ggttatacat caacgcgtag
     2941 tcgatagccc ccgcagagaa agagaagcca gcatggatac ccagcagtgt tgccacaaac
     3001 aggctgatac cggtcagcag tgcgtgcagg aggtacagca gcggtgcaag gaacatgaac
     3061 aggaattcca gcggctcagt cacaccggtc aggaacgcag taacagcaac agaaagcagc
     3121 atcccgccaa ccatcggacg acgctctttc ggtgctgcga agtacatcgc cagcgccgca
     3181 cccggcagac cgaacatcat aatcgggaag aagccggaca tgaacatccc cgcggtgccg
     3241 tcaccggcat agaagcggtt gatatcaccg tggaaaaccg tacccgccgc gttggtgaat
     3301 tcaccaatct ggaaccaggc gatggtgttc agcacctgat gcagaccggt tgggatcagc
     3361 agacggttga tgaaaccaaa gataccggaa cccagcgcgc ccgcagaaac gatccactcg
     3421 cctcctgcat ggatagcgtg ctgtaccggc ggccagacgt aaccaaaaat ggccgccagc
     3481 accaggcaga agaagccggt ggcaatcggc acaaagcgtt tgccgccgaa gaaactcagg
     3541 aagtccggca gtttaatatc ggaccaacgg ttataggctg cgccaccaac cagaccggta
     3601 atgatacccg ccagtacacc catgttaatt tctgggttga tggtcaccat cgctttggtt
     3661 aacacaaagt aacctaccgc acccgccagc gccgccgcac ctgcgctgtc tttcgaccag
     3721 ctggatgcca caccgatggc gaagattaat gcgaggttat caaaaatcgc accgcccgcc
     3781 tgggcaataa acgcaacgtt aagtaaatct ggctgaccga atcgcagcaa cagtgccgcc
     3841 accggcagca ccgcgatagg gagctgtaac gccctaccga gtcgctggaa aaaacctaaa
     3901 atattcatct tattccccct acgagaaccc tatttggctc gtttaaagcc gtatttttat
     3961 tttgctgcaa attgtactgc cgatgttctg taatcagatt gttagatcat ctgctacaga
     4021 gtgtgtgaaa atttaattcg tatcgcaaat taaacgcgtg tcttttgtga gttttgtcac
     4081 caaatatcgt tatcatcact cccttttact ggctaaagca gaaaacttat tttatcattc
     4141 aaaaaatcag gtcggattga cgcctgtctg cgcaaatcca ggttacgctt aaagatgcct
     4201 aatccgccaa cggcttacat tttacttatt gaggtgaata atgagactga tccccctgac
     4261 taccgctgaa caggtcggca aatgggctgc tcgccatatc gtcaatcgta tcaatgcgtt
     4321 caaaccgact gccgatcgtc cgtttgttct gggcctgccg actggcggca cgccgatgac
     4381 cacctataaa gcgttagtcg aaatgcataa agcaggccag gtcagcttta agcacgttgt
     4441 caccttcaac atggacgaat atgtcggtct gccgaaagag catccggaaa gctactacag
     4501 ctttatgcac cgtaatttct tcgatcacgt tgatattcca gcagaaaaca tcaaccttct
     4561 caacggcaac gccccggata tcgacgccga atgccgccag tatgaagaaa aaatccgttc
     4621 ttacggaaaa attcatctgt ttatgggcgg tgtaggtaac gacggtcata ttgcatttaa
     4681 cgaaccggcg tcttctctgg cttctcgtac tcgtatcaaa accttgactc atgacactcg
     4741 cgtcgcgaac tctcgtttct ttgataacga tgtcaatcag gtgccaaaat atgccctgac
     4801 tgtcggtgtt ggtacattgc tggatgctga agaagtgatg attctggtgc tgggtagcca
     4861 gaaagcactg gcactgcagg ctgcagttga aggttgcgtg aaccacatgt ggaccatcag
     4921 ctgtctgcaa ctgcatccaa aagcgatcat ggtgtgcgat gaaccttcca ccatggagct
     4981 gaaagttaag actttaagat atttcaatga attagaagca gaaaatatca aaggtctgta
     5041 attgttatcc ctgccctggc tccttgctca gggcaatatt ttttaaaatc gggggtcaga
     5101 atgtatgcat taacccaggg ccggatcttt accggccacg aatttcttga tgaccacgcg
     5161 gttgttatcg ctgatggcct gattaaaagc gtctgtccgg tagcggaact gccgccagag
     5221 atcgaacaac gttcactgaa cggggccatt ctctcccccg gttttatcga tgtgcagtta
     5281 aacggctgcg gcggcgtaca gtttaacgac accgctgaag cggtcagcgt ggaaacgctg
     5341 gaaatcatgc agaaagccaa tgagaaatca ggctgtacta actatctgcc gacgcttatc
     5401 accaccagcg atgagctgat gaaacagggc gtgcgcgtta tgcgcgagta cctggcaaaa
     5461 catccgaatc aggcgttagg tctgcatctg gaaggtccgt ggctgaatct ggtaaaaaaa
     5521 ggcacccata atccgaattt tgtgcgtaag cctgatgccg cgctggtcga tttcctgtgt
     5581 gaaaacgccg acgtcattac caaagtgacc ctggcaccgg aaatggttcc tgcggaagtc
     5641 atcagcaaac tggcaaatgc cgggattgtg gtttctgccg gtcactccaa cgcgacgttg
     5701 aaagaagcaa aagccggttt ccgcgcgggg attacctttg ccacccatct gtacaacgcg
     5761 atgccgtata ttaccggtcg tgaaccgggc ctggcgggcg cgatcctcga cgaagctgac
     5821 atttattgcg gtattattgc tgatggcctg catgttgatt acgccaacat tcgtaacgct
     5881 aaacgtctga aaggcgacaa actgtgtctg gttaccgacg ccaccgcgcc agcaggtgcc
     5941 aacattgaac agttcatttt tgcgggtaaa acaatatact accgtaacgg actttgtgtg
     6001 gatgagaacg gtacgttaag cggttcatcc ttaaccatga ttgaaggcgt gcgtaatctg
     6061 gtcgaacatt gcggtatcgc actggatgaa gtgctgcgta tggcgacgct ctatccggcg
     6121 cgtgcgattg gcgttgagaa acgtctcggc acactcgccg caggtaaagt agccaacctg
     6181 accgcattca cacctgattt taaaatcacc aagaccatcg ttaacggtaa cgaggtcgta
     6241 actcaataag agaaagtatg acaccaggcg gacaagctca gataggtaat gttgatctcg
     6301 taaaacagct taacagcgcg gcggtttatc gcctgattga ccagtacggg ccaatctcgc
     6361 ggattcagat tgccgagcaa agccagcttg cccccgccag cgtaaccaaa attacgcgtc
     6421 agcttatcga acgcgggctg atcaaagaag ttgatcagca ggcctccacc gggggccgcc
     6481 gcgctatctc catcgtcacc gaaacccgca atttccacgc aatcggcgta cggcttggtc
     6541 gtcacgacgc caccatcacc ctgtttgatc tcagcagcaa agtgctggca gaagaacatt
     6601 acccgctgcc ggaacgtacc cagcaaacgc tggaacatgc cctgttgaat gccattgctc
     6661 agtttattga tagctaccag cgcaaactgc gcgagctgat cgcgatttcg gtgatcctgc
     6721 cagggcttgt tgacccggac agcggcaaaa ttcattacat gccgcatatt caggtagaaa
     6781 actgggggct ggtagaagca ctggaagaac gttttaaagt gacctgtttc gttggtcacg
     6841 atatccgtag tctggcgctg gcggagcact acttcggtgc aagtcaggat tgcgaagact
     6901 ccattctggt gcgtgtccat cgcggaaccg gggccgggat tatctctaac gggcgcattt
     6961 ttattggccg caacggcaac gtcggtgaaa ttggccatat tcaggtcgaa ccgctgggtg
     7021 aacgctgcca ctgcggcaac tttggctgcc tggaaactat cgctgccaac gccgctattg
     7081 aacaacgggt gttgaatctg ttaaagcagg gctaccagag ccgcgtgccg ctggacgact
     7141 gcaccatcaa aactatctgc aaagccgcga acaaaggcga tagcctggcc tcggaagtga
     7201 ttgagtatgt cggtcgtcat ctgggcaaaa ccatcgccat tgctatcaac ttatttaatc
     7261 cgcaaaaaat tgttattgcc ggtgaaatca ccgaagccga taaagtgctg ctccctgcta
     7321 ttgaaagctg cattaatacc caggcgctga aggcgtttcg cactaatctg ccggtggtac
     7381 gttctgagct ggatcaccgc tcggcaatcg gcgcttttgc gctggtaaaa cgcgccatgc
     7441 tcaacggtat tttgctccag catttgctgg aaaattaatg tgcttttata gtggcgctta
     7501 ttgttgtcaa tattctgggt agtccatgac cattaaaaat gtaatttgcg atatcgacgg
     7561 cgtgctgatg cacgataacg tcgccgtacc gggtgcagcg gaatttttgc acgggattat
     7621 ggataaaggc ctgccgctgg tgttgctgac caactatcct tcgcagaccg ggcaagatct
     7681 ggcgaaccgc tttgccactg ccggtgtcga tgtgccggat agcgtgtttt atacctctgc
     7741 gatggcgact gccgatttcc tgcgtcgcca ggaaggcaag aaagcgtatg tggtgggcga
     7801 aggcgcactg attcatgaac tgtacaaagc cggtttcact attaccgatg tgaaccctga
     7861 ttttgtgatt gttggcgaaa cgcgttccta caactgggac atgatgcata aagcggccta
     7921 tttcgtcgct aacggtgcac gctttatcgc caccaacccg gacacccacg ggcgcggttt
     7981 ttatcccgct tgtggcgcgt tgtgtgcagg gattgagaaa atctccgggc gcaaaccgtt
     8041 ctatgttggt aagcccagcc cgtggatcat ccgcgcggca ttaaacaaaa tgcaggcgca
     8101 ttcggaagaa acggtgattg tcggcgataa cttgcgtacc gatattctgg cgggcttcca
     8161 ggcgggtctg gagacgattc tggtgctttc tggtgtttca tcgctcgacg atatcgacag
     8221 tatgccgttc cgcccaagct ggatttaccc gtcggtcgct gaaatcgacg ttatctgaat
     8281 agcaaccacg tctcagggcg tggtttctta tttttaccca tcattaaaat catcccattc
     8341 aataaaactc gccaaaaatt atcgatccat caataaatac atcaccacac catccccttt
     8401 ttcacaaatc agcaatcacc attacgtttt ttatttttgc actgccaaat cgcttgcgca
     8461 cggtgctgct ttgcggcatt tttttaaaca agcaaacaca acaagcaaca aataccaggt
     8521 taacggagaa ggttatgtgt tcaatttttg gcgtattcga tatcaaaaca gacgcagttg
     8581 agctgcgtaa gaaagcactc gagctgtcac gcctgatgcg tcatcgtggc ccggactggt
     8641 ccggtattta tgccagcgat aacgccattc tcgctcacga acgtctgtca attgttgacg
     8701 ttaacgcggg ggcgcaacct ctctacaacc aacaaaaaac ccacgtactg gcggtaaacg
     8761 gtgaaatcta caaccaccag gcattgcgcg ctgaatatgg cgatcgttat cagttccaga
     8821 cagggtctga ctgtgaagtg atcctcgcgc tgtatcagga aaaagggccg gaatttctcg
     8881 acgacttgca gggcatgttt gcctttgccc tgtacgacag cgaaaaagat gcctacctga
     8941 ttggtcgcga ccatctgggg atcatcccac tgtatatggg ctatgacgaa cacggtcagc
     9001 tgtatgtggc ctcagaaatg aaatcgctgg tgccagtttg ccgcacgatt aaagagttcc
     9061 cggcggggag ctatttgtgg agccaggacg gcgaaatccg ttcttattat catcgcgact
     9121 ggttcgactt tgatgcagtg aaagataacg tgaccgacaa aaacgagctg cgtcaggctc
     9181 tggaagattc cgttaaaagc catctgatgt ctgatgtgcc ttacggtgtg ctgctttctg
     9241 gtggtctgga ttcctcaatt atttccgcta tcaccaagaa atacgcagcc cgtcgcgtgg
     9301 aagatcagga acgctctgaa gcctggtggc cgcagttaca ctcctttgct gtaggtctgc
     9361 tgggttcacc ggatcttaaa gcagcccagg aagtggcaaa ccatctgggc acggtgcatc
     9421 acgaaattca cttcactgta caggaaggtc tggatgccat ccgcgacgtg atttaccaca
     9481 tcgaaactta tgatgtgacc actattcgcg cttcaacacc gatgtattta atgtcgcgta
     9541 agatcaaggc gatgggcatt aaaatggtgc tgtccggtga aggttctgat gaagtgtttg
     9601 gcggttatct ttacttccat aaagcgccca acgctaaaga actgcatgaa gagacggtgc
     9661 gtaaactgct ggccctgcat atgtatgact gcgcgcgcgc caacaaagcg atgtccgcct
     9721 ggggcgtgga agcgcgcgtt ccgttcctcg acaaaaaatt ccttgatgtg gcgatgcgta
     9781 ttaacccaca ggataaaatg tgcggtaacg gcaaaatgga aaaacacatc ctgcgtgaat
     9841 gttttgagtc atacctgcct gcaagcgtgg cctggcggca gaaagagcag ttctccgatg
     9901 gcgtcggtta cagttggatc gacaccctga aagaagtggc ggcgcagcag gtttctgatc
     9961 aacagctgga aactgcccgc ttccgcttcc cgtacaacac gccgacgtcc aaagaagcgt
    10021 atctgtaccg ggagatcttt gaagaactgt tcccacttcc gagcgccgct gagtgcgtac
    10081 cgggcggtcc ttccgtcgcg tgttcttccg ctaaagcgat tgagtgggat gaggcgttca
    10141 agaaaatgga cgatccgtct ggtcgtgcgg ttggcgttca ccagtcggcg tataagtaag
    10201 aaatgcgaaa cagccccggt gaaatccggg gctgtttcag ttattgaggt gcttgttaat
    10261 tctgatatgc agtaaggaaa tgttccgggt ttgccacatc actgtttgac aactcatcac
    10321 tgtaggccag tatttcatag attaatttcc caccactatt gttgaattcc gaatttcatc
    10381 ctgtacgaca aaagaatggc tgtagggtgg ggaaaaaaca caaagtaaac aataattgac
    10441 gaatatagcg ccacgctgtt cgcaacctaa ccaaacagtc actttcgagc aattttcctt
    10501 gaaaaagagg ttgacgctgc aaggctctat acgcataatg cgccccgcaa cgccgataag
    10561 gtatcgcgaa aaaaaagatg gctacgtagc tcagttggtt agagcacatc actcataatg
    10621 atggggtcac aggttcgaat cccgtcgtag ccaccatctt ttttgcggga gtggcgaaat
    10681 tggtagacgc accagattta ggttctggcg ccgcaaggtg tgcgagttca agtctcgcct
    10741 cccgcaccat tcaccagaaa gcgttgtacg gatggggtat cgccaagcgg taaggcaccg
    10801 gtttttgata ccggcattcc ctggttcgaa tccaggtacc ccagccatct tcttcgagta
    10861 agcggttcac cgcccggtta ttggggtatc gccaagcggt aaggcaccgg tttttgatac
    10921 cggcattccc tggttcgaat ccaggtaccc cagccatcga agaaacaatc tggctacgta
    10981 gctcagttgg ttagagcaca tcactcataa tgatggggtc acaggttcga atcccgtcgt
    11041 agccaccaaa ttctgaatgt gtcgaatacg ttcggcaaat tcaaaaacca atttgttggg
    11101 gtatcgccaa gcggtaaggc accggattct gattccggca ttccgaggtt cgaatcctcg
    11161 taccccagcc acattaaaaa agctcgcttc ggcgagcttt ttgcttttct gcgttcattc
    11221 aatgtcgaat gcgatgttga cacgtcttat ccttcaatgt cggatgcgac gctgccgcgt
    11281 cttatccgac ctacggttgg cacgcatccg gcaatgttgt aaggctacaa ccctaacgca
    11341 tatttcagcg cctgacgttt caacacgcca gcacgctctg ccgccattaa cccgagatta
    11401 cgcacaaaac gcagcggcgg cagattatta ctgaatccgg cataaaacag atccatacca
    11461 ctttgcataa tgaagttatc cgccatgcgc cgcatctggt aacgcttgag gacaggataa
    11521 ctggcccacg cttcaccgta gctgcgggca ttcaccagaa catcaatcag ggcatcgaca
    11581 tcacgataac caagattcac cccctgcccc gccagcggat ggatggtatg cgcggcatcg
    11641 cccaccagcg caagccccgg ctgcacatac tgcaacgcat gtcggcgcgt cagcggaaac
    11701 gcaccagcgg caagcggtgt aacgtaaccc agacgcgacg ggaaatgctt cgcgatttcc
    11761 gcctggagtt gcgccatatt catattctgc aactggcgaa tacgtaccgg agagtcatac
    11821 cacaccagcg atgcccagtt atcaaacaac ggcagaaacg cacgtggtcc gtccggggta
    11881 aattgctgcc aggtgctgtc gccgggatcg ttctcacact gtacgctaat caacatacac
    11941 gactgcgcat actgccaggc atgaacgcca attcccgcca tctgccgcac ctgcgaattt
    12001 gcgccgtcag caccaatcac cagcttcgcg cgaatcactt caccgccctt cagttccagc
    12061 tcctgaagat cattatctcg atgtaatgca atcagcgagc ctggcacacg taacgttact
    12121 ttcggatgcg cttccagcgc ctgccacaat gcctgttgca ggacggtgtt ttccaccata
    12181 tagccaagca gcggcagctt aagttcagcg gcgtcaaaca tcacatgcgc cgtttcccac
    12241 tcccacgttt ccagtctgcg gtaaggatgg caacgcatag cctgtactgc atcccagacc
    12301 cctaaccctt taagcaatga taccgatgcg gcgctgatcg ccgagatccg cacgtccggt
    12361 tggctatcag cgacaaacgg tgcaggttct gcgtgctcga tcaccgttac cgaaaatccg
    12421 tgctgtgcca gccccagcgc cagtgcgccg ccgaccattc ctccgccgac aatggcaatt
    12481 tccgttggtt gatttgtcat ggtcaatcat cctgttagca atatgcttta agtttacagg
    12541 atttttaccc ccaagggctg ataacgctca tactggtcac aacggcagca aagcattaca
    12601 ctatgcgccc tgcattcctg gctactattt cgcaagagca agtcgatgac caaaaaactc
    12661 catattaaaa cctggggctg tcagatgaac gagtacgatt catcgaagat ggccgatctg
    12721 ctggatgcca cccacggcta tcaactgacc gacgtggcgg aagaagcgga tgtgctgctg
    12781 ctgaacacct gctcaatccg cgagaaggct caggaaaaag tcttccatca gttgggtcgc
    12841 tggaaactgt taaaagagaa gaatccagac ctgattatcg gcgtcggtgg ctgcgtggca
    12901 tcgcaagaag gcgagcacat tcgccagcgc gcccactatg tcgatattat ttttgggccg
    12961 caaacgctgc accgtctgcc ggagatgatc aactccgtgc gcggcgaccg cagcccggtt
    13021 gtagatatca gcttcccgga aatcgagaag tttgaccgtc taccggaacc gcgcgccgaa
    13081 gggccgaccg cgtttgtctc catcatggaa ggctgcaata aatattgtac ctactgcgtg
    13141 gtgccttaca cccgtggtga agaggtaagc cgtccgtctg acgatattct gtttgagatt
    13201 gcccagcttg ctgctcaggg cgtgcgtgaa gtcaacctgc tcggtcagaa cgtgaacgcc
    13261 tggcgtggtg aaaactacga cggcaccacc ggatcgtttg ccgatctgct gcgtctggtt
    13321 gctgcgatcg acgggatcga tcgtattcgc tttaccacca gccatccgat cgaattcacc
    13381 gacgatatca tcgaagtgta ccgcgacacg cctgagctgg tgagtttcct gcatctgccg
    13441 gtacagagcg gttccgatcg cattctgaac ctgatgggcc gtacccatac ggcgctggag
    13501 tacaaagcga tcatccgtaa actgcgtgcg gcgcgtccag atattcagat cagttctgac
    13561 ttcattgttg gcttccctgg cgaaaccacc gaagacttcg agaaaacgat gaagctgatt
    13621 gccgacgtca atttcgacat gagctacagc tttatcttct ctgcacgtcc gggtacacca
    13681 gccgccgata tggttgatga tgttccggaa gaagagaaga agcaacgtct gtatattctg
    13741 caagaacgta tcaatcagca agcgatggca tggagccgcc ggatgctcgg caccacccag
    13801 cgtattctgg tagaagggac ttcgcgtaag agcatcatgg agctttccgg gcgtaccgaa
    13861 aataaccgcg tggtcaactt cgagggcaca ccggatatga tcggtaaatt cgtcgatgta
    13921 gaaattaccg acgtctaccc gaactctctg cgcggtaaag tggtgcgtac tgaagatgaa
    13981 atgggtctgc gcgtggcaga aacaccggaa tcggtgattg cccgtacccg caaagaaaac
    14041 gaccttggcg tgggttatta tcagccgtaa ttctcaggcc cgccgttccg gtgggccttg
    14101 ttttttctct ctttatcccc atctttatcg caatgcttgc gcctttttcc tgtggcgtga
    14161 ataatttcct tataggccgg tgaatgttca gcttccggcg acatacagat aagaggaacg
    14221 gtttgaacat agacactcgc gaaatcaccc tggagccagc agacaacgcg cgtctgttga
    14281 gcctgtgcgg cccgtttgat gacaacatca agcagctcga acgccgtctc ggcatcgaga
    14341 tcaatcgccg cgacaaccac tttaaactga ccggccgtcc gatttgcgtc accgctgcgg
    14401 cagacattct gcgtagcctg tatgtcgata cagccccgat gcgcggtcag attcaggata
    14461 tcgaaccgga acagatccac cttgcgatta aagaagcgcg agtactggag caaagtgcgg
    14521 agagcgtgcc ggagtacggc aaagcggtca atatcaaaac caaacgcggc gtaattaagc
    14581 cgcgcacgcc aaaccaggcg cagtacatcg ccaatattct cgaccatgac attaccttcg
    14641 gcgttggccc ggcgggtacg ggtaaaacct acctggcagt ggctgcggca gttgatgccc
    14701 tggagcgtca ggagattcgc cgtattctgc tgactcgtcc ggcggtcgaa gccggtgaga
    14761 aactgggctt cctgcctggc gatttaagcc agaaagtaga cccgtatctg cgcccgctgt
    14821 acgacgcgct gtttgaaatg ctgggctttg agaaagttga gaaactgatt gagcgcaacg
    14881 ttattgaagt cgcgccgctg gcctatatgc gtggtcgtac gctgaacgac gcgtttatca
    14941 ttctcgatga gagccagaac accaccatcg aacagatgaa gatgttcctg acccgtatcg
    15001 gttttaactc aaaagcggtt atcaccggcg acgtcacaca gatcgacctg ccgcgtaata
    15061 ctaaatcagg cttacgtcac gccatcgaag tgctggccga tgtcgaagag atcagcttta
    15121 acttcttcca cagcgaagac gtggttcgtc accctgtggt ggcgcgtatc gttaacgcct
    15181 atgaagcctg ggaagaagcc gaacaaaaac gaaaagcggc gctggcggca gaacgcaagc
    15241 gcgaagaaca ggaacaaaaa tgagtcaggt gatcctcgat ttacaactgg catgtgaaga
    15301 taattccggg ttaccggaag agagccagtt tcaaacatgg ctgaatgcgg tgatcccgca
    15361 gtttcaggaa gaatcggaag tgactattcg cgtggtcgat accgccgaaa gccacagtct
    15421 gaatctgacc tatcgcggta aggataagcc gaccaacgtg ctctccttcc cgtttgaagt
    15481 gccgcctggc atggaaatgt cgctactggg cgatctggtt atctgccgtc aggtggttga
    15541 gaaggaagct caggagcaag gcaaaccact ggaggcgcac tgggcgcata tggtggtgca
    15601 cggcagtctg catttgttag gttacgatca catcgaagat gacgaagcag aagaaatgga
    15661 agccctcgaa acagagatta tgcttgctct gggctatgag gatccgtaca ttgccgagaa
    15721 agaataattt gccaggcatt tgactggcag acgtccccgc cgttggtgat taacgacggc
    15781 gttttgatta actaacatga gaacccatac gacgccatga gcgacgacaa ttcacacagt
    15841 agtgacacga taagcaacaa gaagggattt ttctccctgt tactcagcca acttttccac
    15901 ggtgaaccga aaaaccgtga cgaactgctg gcgctgatcc gtgattccgg gcagaacgac
    15961 cttatcgacg aagatacgcg cgatatgctc gaaggggtga tggacatcgc agaccaacgc
    16021 gttcgcgaca tcatgatccc ccgctcccag atgattaccc tgaaacgcaa ccagacgctg
    16081 gacgaatgcc ttgatgtcat catcgagtcc gcccactcac gtttcccggt gattagcgaa
    16141 gacaaagatc acattgaagg gattctgatg gcgaaagact tgctgccgtt tatgcgcagc
    16201 gatgctgaag ccttcagcat ggacaaagtg ttacgtcagg cggttgtcgt tcctgaaagt
    16261 aagcgcgtag accggatgct gaaagagttt cgctctcagc gttaccacat ggcgatcgtt
    16321 attgacgaat tcggtggggt ttccggtctg gtgaccattg aagacatcct ggaactgatt
    16381 gttggtgaga ttgaagacga gtatgacgaa gaagatgata tcgacttccg tcagctgagt
    16441 cgtcatacct ggaccgtgcg cgcactggct tccattgaag acttcaacga agcgttcggc
    16501 acccacttta gcgatgaaga agtcgacact atcggtggtc tggtgatgca ggcgtttggg
    16561 catcttccgg cgcgtggcga aactatcgac atcgacggtt accagttcaa agtggcgatg
    16621 gccgatagtc ggcgtattat tcaggttcat gtcaaaatcc cggatgactc accccagcca
    16681 aagctggatg aataaaaccg aaactggata gataactaca tggcttttgc ctcattaatt
    16741 gaacgccagc gcattcgcct gctgctggcg ttattattcg gtgcctgcgg aacgctggcc
    16801 ttctctcctt acgatgtctg gcctgcggcg attgtttcgc tgatggggct tcaggcgctg
    16861 acctttaacc gccgcccgct ccagtctgcc gctattggct tctgctgggg atttggcctc
    16921 tttggcagcg gtattaactg ggtctatgtc agcatcgcga cctttggcgg gatgcctggc
    16981 ccggttaaca tcttcctggt ggtgctgctg gcggcgtatt tgtcgctgta taccggactg
    17041 tttgctggcg tgctgtcgcg cctgtggccg aaaaccacct ggctgcgcgt ggcgattgcc
    17101 gcccctgctc tctggcaagt gaccgagttt ctgcgcggtt gggtgctgac tggcttcccg
    17161 tggttacagt tcggctatag ccagattgat ggcccgttaa aagggctggc accaataatg
    17221 ggcgtggaag ccattaactt cctgctgatg atggttagcg gcctgctggc actggcgtta
    17281 gtcaaacgca actggcgtcc gctggtggtg gccgtcgtgc tgtttgccct tcccttcccg
    17341 ctgcgttaca tccaatggtt taccccgcaa ccggagaaaa ccattcaggt ttcgatggtt
    17401 cagggcgata ttccgcaatc actgaaatgg gacggagacc agcttcttaa tacgctgaag
    17461 atttactaca acgcaacggc accgctgctg ggcaaatcat cgttgattat ctggccggag
    17521 tcggcgataa ccgatctgga aattaatcag caaccgttcc tcaaagcact ggacggtgag
    17581 ttgcgtgata aaggtagctc gctcgtaacc gggattgtcg acgcgcgtct caataagcag
    17641 aaccgctacg atacctacaa caccatcatc acactgggta aaggtgcacc gtacagctac
    17701 gaatcagccg atcgctataa caaaaaccat ctggtgccgt ttggcgagtt tgtcccgctg
    17761 gagtcgattc tgcgtccgtt agcaccgttc tttgatctgc cgatgtcgtc gttcagccgt
    17821 gggccatata tccagccgcc gctgtcggta aatggtattg agcttactgc ggccatttgc
    17881 tacgagatca ttcttggcga gcaagtgcgc gataacttcc gcccggatac cgactatctg
    17941 ctgactatct ccaacgatgc gtggtttggt aaatctattg gtccatggca acacttccag
    18001 atggcgcgaa tgcgtgcgct ggagctggcg cgcccactgt tgcgcagcac caacaacggc
    18061 attacggcgg tgattggccc gcagggtgag attcaggcga tgatcccgca gttcacccgc
    18121 gaggcgttaa ccactaatgt gacgccgacc accggactca caccatacgc acgtaccggc
    18181 aactggccgc tgtgggtgct gacggcactg tttggttttg ccgctgtgct gatgagtctg
    18241 cgagcacgta aacgttgatg tgcctgatgt atcctgcatg agcaaaacgt actttctcat
    18301 tgagctttgg ggcatggtta tcatgcccct ttttcttacg gttcagggat acaagccaca
    18361 ttgccatatt ggggatgacg aataattgcc tgcggatcaa gcgagcgcgt cctgtccagt
    18421 tggatcaacc cgttcttcgc cataacaaat atttttctac catccgctga aaaacgaagg
    18481 tgattaacgt agctggcagg ataaacgcca ataatctgcc cggcattaat attgagcagg
    18541 atgacataac cttgcaggcc atatgacacc aacgctaaat actgaccgtc cgggctaata
    18601 ccctcaacgg aataaacgct cctgctacac cattgcgtca taatgctggc ttgttgatcc
    18661 cctacgctta tacgtgaaat cccctggctg tcggcaaaaa ttacatcccc gttttcaggt
    18721 cgaaaacgca tcgccgatat cgttcctcgc gcatcactag ccggatagct ttctttctga
    18781 tgcgtgatgg tattgatgcg tacaatgtca cctctgacca taccgggtat caacaatatt
    18841 ttttcatccg gggaaagagc cataacccag gcacaaggtt tattactcgt ccccgtcccg
    18901 tcgtatttct ccagcgggat atccgccaca tgctgataag tcaccagatc aatcgctgac
    18961 acactattac tggtgcgatt catcacccag gcttttgtac catcacgcga gaggataagg
    19021 ctgtcaggat gtgcaccggt ggtaatcgac tgaataatct cccctgtcgc cacgttaatg
    19081 accgataacg cattcgcttc aaatgaagtc gcataaatca acttttcatc cgggcttaat
    19141 gccagcgatc tgacgccctg atctttacag ccaccacaat gtttgactga cttttctttc
    19201 gctatgggcg ctgtaaaggt atgcaggatt ttcttttgtc cgaggtcgaa aacaacaatg
    19261 ctgttggtgt tttcaattgc ggcatacaac atattaccat cccggctgat cgccatatca
    19321 tggatatcgg caatctcagc gagcaaatca ccactggcct gttcacgact taccggagcg
    19381 gataatgttt ccctgagggc gttgcgttcc tccgctaact gctgcttttt ttgccgtcgc
    19441 tcttgttcaa tctcctgtgg ataaaacgct tcacgcaggc tttgcctgac ttgtaaaact
    19501 gactcatcca tctgctgctg cgcaagccag atagccccgg tcataagaag actgcccgca
    19561 acaataatcc cgccttccgt aacgagtcgg ttttgcccag ataaacgcgc gctcagccaa
    19621 cccaacgcgc cacccgcaat caggccgcca atgtggcaag cgttatctat tcctgtttgc
    19681 cgggcaccgt acaacagtgt cagcgccacc atacccaaca gaggaaatac tcgccgctgg
    19741 tttttatggt atgtgcccgt acctgaagca aactgtgttg ctatcgctgc accagctatt
    19801 cccagaatcg ccccggatgc cccaacgctg atgactatct gctcactttc gcgtaacgca
    19861 taacaggcgc tgatgagccc accaaagacg ccggagaata accaggtaat caggagtcgc
    19921 catttcccca gaatctgttc cgccacgcct cccacgacca ggaatgcaaa catattcatc
    19981 agcagatgag aaacactact gtgcagaaag atactgctca ataagcgcca gggttcaccc
    20041 gacaaagtaa gaggtgcaac gtttgcgccg agatggataa gtgcggattg atcgacctct
    20101 tcataactca tccattgtgc gagataacaa aatatcccaa tacagagtgc aaaaaggata
    20161 agggttattg cgggtaattg aacatttaac ggcttaaccg aagatgcaga cataaggtgt
    20221 ttcttccttg aaaagacagg taaaaagcat ttttcccgtg tgcagttatc atcccggcaa
    20281 acgtcccatg ccgaaaagat aattctgtcc gaaatatgac tttttcagcc taaaattaca
    20341 atcgtaatta attcgcccct aagtattttt tggcatgtaa gttgctttat ttactgtgta
    20401 agcgatgaaa tcagccgcga cgcaaccagg ttgcaccacc acaaatcagc ggtagcacca
    20461 aaaaggtgcg gtttaagaat tttgcttcgg aatggtgcgg cacatctcgc aaaaacaaac
    20521 aataaaacag caatatcttt acatttagcc aaactaaatg ttacctaaca ggcacaacac
    20581 tgcacaataa agttgcagac gataacaaca caaacactca caacgggtat ccatgcgttc
    20641 ttaacgcaga agataaagga gttggatatg caattacgta aacctgccac agcaatcctc
    20701 gccctggcgc tttccgcagg actggcacag gcagatgacg ccgccccggc agcaggcagt
    20761 acgctggaca aaatcgccaa aaacggtgtg attgtcgtcg gtcaccgtga atcttcagta
    20821 cctttctctt attacgacaa tcagcaaaaa gtggtgggtt actcgcagga ttactccaac
    20881 gccattgttg aagcagtgaa aaagaaactc aacaaaccgg acttgcaggt aaaactgatt
    20941 ccgattacct cacaaaaccg tattccactg ctgcaaaacg gcactttcga ttttgaatgt
    21001 ggttctacca ccaacaacgt cgaacgccaa aaacaggcgg ctttctctga tactattttc
    21061 gtggtcggta cgcgcctgct gaccaaaaag ggcggcgata tcaaagattt tgccgacctg
    21121 aaagacaaag ccgtagtcgt cacttccggc actacctctg aagttttgct caacaaactg
    21181 aatgaagagc aaaaaatgaa tatgcgcatc atcagcgcca aagatcacgg tgactctttc
    21241 cgcaccctgg aaagcggtcg tgccgttgcc tttatgatgg atgatgccct gctggccggt
    21301 gaacgtgcga aagcgaagaa accagacaac tgggaaatcg tcggcaagcc gcagtctcag
    21361 gaagcctacg gttgcatgtt gcgtaaagac gatccgcagt tcaaaaagct gatggatgac
    21421 accatcgctc aggtgcagac ctccggtgaa gcggaaaaat ggtttgataa atggttcaaa
    21481 aatccaattc cgccgaaaaa cctgaacatg aatttcgaac tgtcagacga aatgaaagca
    21541 ctgttcaaag aaccgaatga caaggcactg aactaattac aagaaccagg ggcggaaatt
    21601 ccagccctct cgattgttac gtagcacgga cagactatac gcctgatggt cgttccccat
    21661 cgggcctgaa aaccgcaata cgctgggtaa caatcttcga gggtagcagt taacgctgct
    21721 accctctttt ttctggagta gatttatgtc tatagactgg aactggggta tttttttaca
    21781 acaagccccg ttcggcaaca ccacctatct tggttggatc tggagtggtt ttcaggtgac
    21841 gatcgctttg tcgatctgcg cctggattat cgctttcctc gtcggttcat ttttcggcat
    21901 tttacgtacc gttccaaacc gttttctctc cggtctgggt acgttgtatg tcgaactgtt
    21961 ccgcaacgtg ccgttgattg tgcaattctt tacctggtat ctggtgatcc cggagctgtt
    22021 gccggagaaa atcggcatgt ggtttaaggc cgagctggat cccaatattc agtttttcct
    22081 ttcatccatg ctctgcctgg ggctgtttac cgccgcccgt gtttgcgaac aggtacgcgc
    22141 cgccattcag tcgttgccgc gtgggcaaaa aaatgccgcg ctggcgatgg gcctgacgct
    22201 accgcaggct tatcgatacg tactgctgcc taatgcttat cgagttatcg tcccgccgat
    22261 gacttccgag atgatgaacc tggtgaaaaa ctccgccatc gcctcgacta ttggtctggt
    22321 ggatatggcg gcgcaggcgg gtaaattgct tgattactca gcccacgcct gggagtcgtt
    22381 taccgctatt acgctggcgt atgtgttgat taacgcggtt atcatgctag taatgacgct
    22441 ggttgaacgt aaagttcgcc tgcctggcaa catggggggc aaataatgta cgagtttgac
    22501 tggagttcca ttgtcccttc cctgccatat ctgctcgacg ggctggtgat cacgctgaaa
    22561 atcaccgtca cggcagtcgt gattggtatt ttgtggggca ccatgctggc ggtgatgcgc
    22621 ttgtccagct ttgcgcccgt agcgtggttt gccaaagcct acgttaacgt attccgctcg
    22681 attcctttag ttatggtttt gctgtggttt tacctgatcg tgccaggttt tctgcaaaac
    22741 gtgctgggat tatcaccaaa aaatgatatc cgtttaattt cggcgatggt ggcgttttcg
    22801 atgtttgaag cggcctacta ttcagagatt atccgtgctg gtattcaaag tatttctcgt
    22861 ggtcagtcga gcgccgcgct ggcgttggga atgactcact ggcagtcgat gaaactgatt
    22921 attctgccgc aggcgttccg cgcgatggtg ccgcttctgc tcactcaggg catcgtactg
    22981 ttccaggata cctcactggt gtatgtgtta tctctggccg atttcttccg taccgcctca
    23041 accattggtg agcgtgatgg tacgcaggtt gagatgatcc tgtttgccgg atttgtttat
    23101 ttcgttatta gtcttagcgc gtcgttgttg gtcagctact tgaaaagaag gacagcataa
    23161 tgattaccct gaaaaatgtt tcaaaatggt atggtcactt tcaggtgctg accgactgct
    23221 caaccgaagt gaaaaaaggc gaagtggtgg tggtttgcgg cccgtctggt tccggcaaat
    23281 caacgctgat taaaaccgtc aacggcctcg aaccagtgca gcaaggtgaa atcaccgtcg
    23341 atggtatcgt ggttaatgac aagaaaaccg atctggcaaa gctgcgttcc cgcgtcggga
    23401 tggtattcca gcatttcgag ctgttccctc atctgtcgat tatcgaaaac ctgaccctgg
    23461 cgcaggtgaa agtgcttaaa cgcgataaag cgcccgctcg tgaaaaagca ttgaaattgc
    23521 tggagcgcgt gggactttcc gcacatgcca ataaatttcc ggcgcagctt tccggcggtc
    23581 agcagcagcg tgtggcaatc gctcgcgcgt tgtgtatgga tcctattgcg atgctgttcg
    23641 acgaaccgac atcggcgctg gatccggaga tgatcaacga agtgctggac gtgatggtgg
    23701 aactggcaaa cgaaggaatg accatgatgg tggtgaccca cgaaatgggt tttgcccgta
    23761 aagtggcgaa tcgggtgatc tttatggacg agggtaaaat tgtcgaagac tcgccgaaag
    23821 acgctttctt cgatgatccg aaatcggacc gcgcaaaaga cttcctcgcg aaaatcctgc
    23881 attaatcact ctggcgcgca tacccaattg cgcgccatct cacgctttat cgtttcgccc
    23941 ctcgtatctg ttacatcctg gcgttaccct tgtcgcaaag aagcacacaa caaggagcaa
    24001 caatggcact gccaattctg ttagattgcg acccaggtca tgatgacgct atcgcaatag
    24061 ttctcgccct cgcctcacca gagcttgatg tcaaagcaat tacgtcttcc gccggaaacc
    24121 agacaccaga aaaaacctta cgcaatgttc tgcgtatgct gaccttgctt aatcgcaccg
    24181 atattccggt agccggcggc gcggtaaaac cgttaatgcg tgagttgatt atcgcggata
    24241 atgtgcacgg cgaaagcgga cttgacggcc cggcactacc ggaaccgacg tttgcaccgc
    24301 aaaagtgtac ggcggtggag ctgatggcga aaacgctacg tgaaagtgcg gaacctgtca
    24361 cgattgtgtc taccggaccg caaactaacg ttgccttgct gctcaatagc catccggaac
    24421 tgcatagcaa aattgctcgt atcgtgatta tgggcggcgc aatggggctt ggtaactgga
    24481 cgcctgcggc tgaatttaat atttacgttg acccggaagc ggcagaaatt gtcttccagt
    24541 cagggatccc ggtggtgatg gccggtctgg atgttactca taaagcacaa atccacgttg
    24601 aagacaccga gcgtttccgc gcgattggca accctgtttc aaccattgtt gccgaactgc
    24661 tggatttctt cctcgaatat cataaagacg aaaaatgggg ctttgtcggt gcaccgctgc
    24721 atgacccatg caccatcgcc tggctgttga aacccgagtt gtttaccact gttgagcgct
    24781 gggttggcgt ggaaacacag gggaaatata gccagggtat gacggttgtt gattattatt
    24841 atctgactgg caataaaccg aatgccactg taatggtcga tgttgatcgt cagggctttg
    24901 ttgatttact ggctgatcgt ctgaaatttt acgcttaaca cacgtcattg attaagatca
    24961 ccacgcgtta cctgggtggt cttaatctgt ttatctcttt ttctttcaag atataaatgc
    25021 aatggataat gcagaactcg ccattggtat cgatctcggc actaccaata gtttaattgc
    25081 cgtctggaaa gacggtgccg cgcaattaat tccaaataag ttcggtgaat atttaacacc
    25141 atccataatt agcatggatg aaaataatca tattttagtc ggaaaaccgg ctgtatcacg
    25201 gcgtacttcg catccggata aaacggcagc gttatttaaa cgtgcaatgg gcactaatac
    25261 caactggcgg ttaggcagcg acacatttaa cgcgccagaa ctgtcctctt tggtattacg
    25321 ctcattaaaa gaagatgccg aagaatttct gcaacgtccg attaaagatg tggtgatctc
    25381 cgttccggct tatttcagcg atgaacaacg caagcatacc cgtttagcag cggagttagc
    25441 cgggttaaat gcggtacgct taattaatga acccacagca gctgcgatgg catatggcct
    25501 gcatacgcaa caaaataccc gctcgctggt ttttgatctc ggtggcggca cgtttgacgt
    25561 cacggtgctc gaatacgcca cgccggtgat tgaagttcac gcctccgctg gcgataactt
    25621 cctcggtggc gaagatttta cccatatgtt ggtcgatgac gttttaaagc gcgcggatgt
    25681 cgccaggacc acgcttaacg agagtgaact ggcagccttg tacgcctgtg tggaagcggc
    25741 aaaatgtagc aatcaatcgc cattgcacat tcgctggcag taccaggatg aaacgcggga
    25801 gtgcgaattt tacgagaacg aactggaaga tttgtggttg ccgctgctca atcgcttgcg
    25861 agtgccgatt gaacaggcgt tgcgcgatgc acgtctgaag ccgagtcaaa tcgacagtct
    25921 ggtgctggtt ggcggcgcgt cacaaatgcc gctggtgcag cgaatcgccg tgcgtctgtt
    25981 tggcaaatta ccgtatcaaa gttacgaccc gagcaccatt gtcgcgctgg gcgcagcaat
    26041 ccaggccgcc tgccgcttac gcagtgaaga tattgaagag gtgatcctca ctgatatttg
    26101 cccttactcg ttgggcgttg aagttaaccg ccagggcgtt tccggcattt tctcgccaat
    26161 tattgaacga aacaccacag tacccgtgtc gcgtgtagaa acttactcga ccatgcaccc
    26221 ggaacaggat tcaattacgg ttaacgtcta tcagggagaa aaccacaaag ttaaaaataa
    26281 cattctggtt gaatccttcg atgtgccgtt gaagaaaacc ggggcttatc agtcgattga
    26341 tattcgcttt agttatgata tcaacggctt gcttgaagtt gacgtgcttc tggaagatgg
    26401 cagcgttaag tccagagtca ttaatcatag cccggtaaca ttgagcgcgc agcagattga
    26461 agagagtcgg acgcggttat ccgcattgaa aatttatccg cgcgatatgc tcatcaaccg
    26521 cacctttaag gccaaactgg aagagttgtg ggcgcgggcg ctgggtgacg agcgagaaga
    26581 gattggccgg gtgatcaccg attttgatgc ggcgttgcag tcaaacgata tggcccgcgt
    26641 cgatgaggtc aggcggcggg cgagcgttta tttagccatt gagacctcat aatcacggct
    26701 gccggatgcg gcgtaaatgc tgcatccggc atcggcgcaa aaaacacatc gtcaactaaa
    26761 caacatcgcg attttgccga gagcataacc tataaccagc accgttaaac tgattttgat
    26821 aatgctggta atgctctgtg gtttttcacc ctcatcattt ttttcttcat ggttttcagg
    26881 tgttggctca tcgacatctt catcctccgg ctcgagtttt actttcgtca acagcatccg
    26941 ggtgataatg gcctctaatg gatgagcatc tccgccttcc agccttgtat taagacgggc
    27001 cagcatctct tgtttcggaa acgcttcaag tactacctgt tccgctaccc gatacaaatc
    27061 cagtaacggt gaatcttctt ctgtctcgct accgactctc gatttccacc acgcccatgc
    27121 tttgcgtttg aagaataacg actcaataaa cgattgacct aacggcgata attttccgct
    27181 atgtaaaatt tccgcccata acagccgcgt ccgggcgctc tcaccaggaa catatttcag
    27241 cggttgacct tgcgcatcta ccatgctccg cgcttcgata accatcacta ataacgcgaa
    27301 ataatccggg caatgacgct tgctccactg caacaacaaa ttatcagctt gtgtagatgg
    27361 gtaactttcc cggtacgcgc agagatcggc aagcaggctt tccccctcgc cgcagtacaa
    27421 cctttgagca cacaagtaat atcgggcatc ttcgctttct ggttcttcca tttgccactg
    27481 tttggctaca ggaatgagtt ccgccatacc tgtttgcacc gagctgtacc agcgcaacag
    27541 tttgcgatgt aactttgcat catcaggaat gaaccacgga ccttccatcg ccaaaaacgc
    27601 agtgacgtag tcaggatggt aacggcagca acgttccagc gcgaagtaat actcaacagt
    27661 ctggttctgc aacgcaacgg gatattccag aaggctaagg aaatcgaaac aatcaccagc
    27721 ctgaatggcc tcaagaaaag cctccctttc ttcctcatcc attccttcgc tgtcattaaa
    27781 cgaatgccag ttcaaacgct ctgccagcaa agaagcacaa tttaatgaaa gatatcgcgc
    27841 ttctatagcg attgcacaca gcggccagcg taattgatcg acatcctcca tgttccaggt
    27901 atttaattgc tgaataaatt tctgccaggc ggagggctga aaacgatcac tttccgaatc
    27961 cagtaatgtc ctgaatgcac gcagtatttc atgttcagcg gcggcatctt tttcttcatc
    28021 atctgcttcc tcgacaggat ttaccgccag tcgcagcgcc tcttcatagg cctggcgtaa
    28081 tgctttaaac ccctgcggat cggtttcggg atgacataac ggtaagcgag caagataagc
    28141 ctggcggata atgtctattt gcgtcgtgct ttcaatttcg aggatttgcc aacatgtttt
    28201 cataggtcgc tcacatcctt aagcgttacc gggcattcag gctggacagt taatggctgc
    28261 cagggaagcg tggcatagaa acgatcgctt cccgctgcca gctcttcgaa catccgactc
    28321 ccggcgctat cgtatttgta cagggttagc gcctcgcgta gcgaagtgtt gtcttcgcac
    28381 tgagaagatt gccagtagag acgtcctaac gaataggcgg aaaaatagtg catccagctg
    28441 tgatagtaat ggtgagctcg tacatagacg cgcgattgca gccataaact ctcttcttcc
    28501 gtcagccagt tgttgagcac accgacccgt gacagaaacc ccattcgaac ataatcccag
    28561 gctttgattc ccccttctcc acagcacata gcggtttggg caacgaattg ggtataaatc
    28621 aaccctctct ccgagccacc agcaattaat gctttccact cttcagggga agagcgatgc
    28681 catgtcagat ataagccagc taaatcattg gcatgaccat catcagtcat ctgataaata
    28741 gtctgaatta aatcatcacg attatttatt ccccaggatg atgagagatc gaattctttt
    28801 ataccggtat acatttttgg cgagtcatgc gcgggtttgc aaccctgatt aagtatttcc
    28861 atgggcgcag aaagcgcaaa aagaagatat tgttcttcct tattcatttg ttaataatcc
    28921 cttaagtgca ttaactgact actttttatt attgatgagt ataaacttga cgctttcaaa
    28981 ataaaaagaa aatcgaagta ttcacatatg aataaaaaat taatgtatat attcgcaata
    29041 tttatagttg cagcaattac ctgtattagc caacccaaga aaacaacgtt gcgtgataaa
    29101 gccatggtga attatgcctt tgattattta agctcaccag gcagtctacc gttaaccacg
    29161 gcagccacgg agctttccgc gattcatggt cactcaacat cgcaatatcg ccttggcgaa
    29221 ttttatcttc atggtagcga cggtaaacca ctggattata cacaggcgag atactggtat
    29281 gagcaatcag cagaacagga aaatccgcgc gcacaaagca aactggggtg gatctacctg
    29341 aaaggcctgg gagtcaaacc cgatacccgt aaagcaattc tctggtataa ggaagcagct
    29401 gaacaagggt atgctcatgc tcaatatact ttgggtttga tctacagaaa tggcactggt
    29461 attaatgtta accattatga atctcaaaaa tggttaaaac tggccgccaa acaacattac
    29521 aaaaatgcgg aaagattact tgccggactt cccgcacatt aacgtcgact gtcacccgaa
    29581 gagatggagt attttcccta tcaaaccggc aaagataaaa atgtagaaaa taattttgat
    29641 gacgcccaga cagcccagcc cgccatcgtc aacgggcatt ggctgcggtt gcggctccgg
    29701 ctcgacggtt tcctgaccgg taaaattcgc cgcctgaatt aataatcgac gaataattaa
    29761 tgcttccagt ggacaacgat cttctgcatc aaggcggctc acaatcggca ttaatgattc
    29821 ttcggttggt tggctggcct gcactaattg tttggcaact aaatataatg gccataaggg
    29881 atgttctgta tcacgcgtga gtgagatccg cggcttacgt cctttgtgca aaaccatctc
    29941 gaccagtgaa cgccccagag cagagagttt ttcatcgctg agtatttcgt ataaatgaaa
    30001 acgcgtacgg gcgctgtcgc cggggacata aagtagcggt tttcccttat cgttaaccag
    30061 atcacgcgct tcaatcatca tcaccactaa cgggtaataa tcgacccgat gctgacggca
    30121 ccatagcaac attaaagcat ccgcctgggt ggagggatac tcgcgccagt agtcacaaag
    30181 ttctgccagc aagctttcgc cttctccaca atagacgcgt tgcgcgtatt cataataacg
    30241 cgcatcttca ttatcaggtt cagcttcccg ccacgaccgg gcgtaatcca gaagttcggg
    30301 gatatccagg cgacctgcgc tataccagcg gagtaaattt ctgtgcagtt ttgcatcatc
    30361 ggggatgatc actgcgcgat gttgcgctaa atacaatgtc agccattctg gatgcgatga
    30421 ccaaatccga gcgaacattt ggtaaaaatc gatggtgtca ttttgcacgg caacgggcag
    30481 atgcagaatg gtctggaagt taaaaacatt ccccttcgca atggcatata aaaaggattc
    30541 cagttcttct tcgtctattt ccccgacgtt ttcctcctgc aaccaccgca atctttctgc
    30601 taataacacc acgcactcga aggataaatg ggcagtgttc atggcgattg tgcacagcga
    30661 ccagcgtaat tcatcaatat cttccatcga gcaataattt aattgctgaa tgaatcgctg
    30721 ccaggcggag ggcagaaaac gttcgctatc agaggcaagt aacgcgcgaa aggcgagcag
    30781 aatttcatgt tctgctacct catattcttc tggttgccaa acagatttag caggcgactg
    30841 cgcaatccgt agcgcctcct catacgcctg acgaagttgt ttaaaaccct gcggatcggt
    30901 ttctggatga aaggacggta acagcgccag ataagcgcgg cggataatat cgacgtcagt
    30961 cgtttcctct atttcgagga tcttccagca atttttcata ggtcgctcat atccttaagc
    31021 gtttccgggc atgcagaata gtaagccaat ggttgccagg acaaggtagg gtaatacgca
    31081 ttatcactgg caaataattg ataaaacata tcattgccgg aatcgtcgta ttctttacgc
    31141 gctaagaatt cacgtaataa ctgcagatta tcatcttcgg gagaatgcca atattgccga
    31201 ccaaaggtat aaccggcaaa gtattgccgc cagttacgat aataacgtag agcccgtaga
    31261 tgaatgcgcg actggatcca tagactttct tcttcgctca accagttatt aagtacgccc
    31321 attcggctta aaaaacccat ccggacataa tcccaggctt taattcctcc ctcaccacag
    31381 cattcggcgg tgctggcgac aaattgcgca taagcctgac cttgctcatt tagttcagca
    31441 agatagttac gccattcaca tggagaataa cgaaaccagc ggtgataaaa ccctgccaac
    31501 cgagcggcat gaccgttctc gatcattcgg tgaattgtgc gcagcaggtc atcacggtca
    31561 tcaattcccc atgaggaaga aaggtcaaaa aagcatgtac ctaaatacat tttgggtgat
    31621 gaatgcgaag gtaaacagca ttcattgcgt atttccatcg gagtggaaag tgcaaacaat
//
LOCUS       DAZFQD010000049        31482 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.049, whole genome shotgun sequence.
ACCESSION   DAZFQD010000049 DAZFQD010000000
VERSION     DAZFQD010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31482
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.049"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(90..1487)
                     /gene="ccp"
                     /locus_tag="ACHKTW_002282"
     CDS             complement(90..1487)
                     /gene="ccp"
                     /locus_tag="ACHKTW_002282"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HFO6463295.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHALNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLEVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
     gene            1892..3541
                     /gene="treF"
                     /locus_tag="ACHKTW_002283"
     CDS             1892..3541
                     /gene="treF"
                     /locus_tag="ACHKTW_002283"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005993 - trehalose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HFO6463296.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGVYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKMI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
     gene            complement(3592..4194)
                     /gene="yhjB"
                     /locus_tag="ACHKTW_002284"
     CDS             complement(3592..4194)
                     /gene="yhjB"
                     /locus_tag="ACHKTW_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFO6463297.1"
                     /translation="MQIVMFDRQSIFIHGMKISLQQRIPGVSIQGASQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
     gene            4714..5613
                     /gene="rcdB"
                     /locus_tag="ACHKTW_002285"
     CDS             4714..5613
                     /gene="rcdB"
                     /locus_tag="ACHKTW_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO6463298.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSELDGLFQQDATSISGKLRIDIPPGI
                     AKSLLLPRLSEFLYLHPGIELELSSHDRPVDILHDGFDCVIRTGALPEDGVIAPPLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAIVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLAAGLAGLGIIQIPRIAVREALRAGRLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEHLD"
     gene            5662..6675
                     /gene="yhjD"
                     /locus_tag="ACHKTW_002286"
     CDS             5662..6675
                     /gene="yhjD"
                     /locus_tag="ACHKTW_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HFO6463299.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            7086..8408
                     /gene="yhjE"
                     /locus_tag="ACHKTW_002287"
     CDS             7086..8408
                     /gene="yhjE"
                     /locus_tag="ACHKTW_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO6463300.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVASI
                     LGASVAPYIAAWLQANYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(8642..10702)
                     /gene="yhjG"
                     /locus_tag="ACHKTW_002288"
     CDS             complement(8642..10702)
                     /gene="yhjG"
                     /locus_tag="ACHKTW_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFO6463301.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGDLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYVFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLIYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLDIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(10772..11539)
                     /gene="pdeH"
                     /locus_tag="ACHKTW_002289"
     CDS             complement(10772..11539)
                     /gene="pdeH"
                     /locus_tag="ACHKTW_002289"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HFO6463302.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFIEHGLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPIETLNTAVLAL"
     gene            11771..12700
                     /gene="kdgK"
                     /locus_tag="ACHKTW_002290"
     CDS             11771..12700
                     /gene="kdgK"
                     /locus_tag="ACHKTW_002290"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HFO6463303.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLESEQSAAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRANG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEGLVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVRLT
                     GGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            complement(12796..14292)
                     /gene="yhjJ"
                     /locus_tag="ACHKTW_002291"
     CDS             complement(12796..14292)
                     /gene="yhjJ"
                     /locus_tag="ACHKTW_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFO6463304.1"
                     /translation="MQGTKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLADSTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASNSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAAATSVATDDVHPEVTDIPPAQ"
     gene            complement(14513..15799)
                     /gene="dctA"
                     /locus_tag="ACHKTW_002292"
     CDS             complement(14513..15799)
                     /gene="dctA"
                     /locus_tag="ACHKTW_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HFO6463305.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            complement(15982..17970)
                     /gene="hmsP"
                     /locus_tag="ACHKTW_002293"
     CDS             complement(15982..17970)
                     /gene="hmsP"
                     /locus_tag="ACHKTW_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HFO6463306.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            complement(18052..21525)
                     /gene="bcsC"
                     /locus_tag="ACHKTW_002294"
     CDS             complement(18052..21525)
                     /gene="bcsC"
                     /locus_tag="ACHKTW_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex outer membrane
                     protein BcsC"
                     /protein_id="HFO6463307.1"
                     /translation="MRKFTLNIFTLSLGLAVMPMVEAAPTAQQQLLEQVRLGEATHRE
                     DLVQQSLYRLELIDPNNPDVVAARFRSLLRQGDIDGAQKQLDRLSQLAPSSNAYKSSR
                     TTMLLSTPDGRQALQQARLQATTGHAEEAVASYNKLFNGAPPEGDIAVEYWSTVAKIP
                     ARRGEAINQLKRINADAPGNTGLQNNLALLLFSSDRRDEGFAVLEQMAKSNAGREGAS
                     KIWYGQIKDMPVSDASVSALKKYLSIFSDGDSVAAAQSQLAEQQKQLADPAFRARAQG
                     LAAVDSGMAGKAIPELQQAVRANPKDSEALGALGQAYSQKGDRANAVANLEKALALDP
                     HSSNNDKWNSLLKVNRYWLAIQQGDAALKANNPDRAERLFQQARNVDNTDSYAVLGLG
                     DVAMARKDYPAAERYYQQTLRMDSGNTNAVRGLANIYRQQSPEKAEAFIASLSASQRR
                     SIDDIERSLQNDRLAQQAEALENQGKWAQAAALQRQRLALDPGSVWITYRLSQDLWQA
                     GQRSQADTLMRNLAQHKPNDPEQVYAYGLYLSGHDQDRAALAHINSLPRAQWNSNIQE
                     LVNRLQSDQVLETANRLRESGKEAEAEAMLRQQPPSTRIDLTLADWAQQRRDYTAARA
                     AYQNVLTREPANADAILGLTEVDIAAGDKAAARSQLAKLPATDNASLNTQRRVALAQA
                     QLGDTAAAQRTFNKLIPQAKSQPPSMESAMVLRDGAKFEAQAGDPKQALETYKDAMVA
                     SGVTTTRPQDNDTFTRLTRNDEKDDWLKRGVRSDAADLYRQQDLNVTLEHDYWGSSGT
                     GGYSDLKAHTTMLQVDAPYSDGRMFFRSDFVNMNVGSFSTNADGKWDDNWGTCTLQDC
                     SGNRSQSDSGASVAVGWRNDVWSWDIGTTPMGFNVVDVVGGISYSDDIGPLGYTVNAH
                     RRPISSSLLAFGGQKDSPSNTGKKWGGVRADGVGLSLSYDKGEANGVWASLSGDQLTG
                     KNVEDNWRVRWMTGYYYKVINQNNRRVTIGLNNMIWHYDKDLSGYSLGQGGYYSPQEY
                     LSFAIPVMWRERTENWSWELGASGSWSHSRTKTMPRYPLMNLIPTDWQEEAARQSNDG
                     GSSQGFGYTARALLERRVTSNWFVGTAIDIQQAKDYAPSHFLLYVRYSAAGWQGDMDL
                     PPQPLIPYADW"
     gene            complement(21507..22613)
                     /gene="bcsZ"
                     /locus_tag="ACHKTW_002295"
     CDS             complement(21507..22613)
                     /gene="bcsZ"
                     /locus_tag="ACHKTW_002295"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex periplasmic
                     endoglucanase BcsZ"
                     /protein_id="HFO6463308.1"
                     /translation="MNVLRSGIVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRAAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYLPPTLAQYFTRFGAPWTTLRETNQRLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPSSDAYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            complement(22620..24959)
                     /gene="bcsB"
                     /locus_tag="ACHKTW_002296"
     CDS             complement(22620..24959)
                     /gene="bcsB"
                     /locus_tag="ACHKTW_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HFO6463309.1"
                     /translation="MKRKLFWICAVAMGMSAFPSFMTQATPATQPLINAEPAVAAQTE
                     QNPQVGQVMPGVQGADAPVVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNLEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKNDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKAPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETRSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIRESGINSLRVGDVYYVGHLPWFERVWYAL
                     ANHPILLAVLAAISVILLAWVLWRLLRIISRRRLNPDNE"
     gene            complement(24970..26070)
                     /locus_tag="ACHKTW_002297"
                     /pseudo
     CDS             complement(24970..26070)
                     /locus_tag="ACHKTW_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312440.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
     gene            complement(26342..26926)
                     /locus_tag="ACHKTW_002298"
     CDS             complement(26342..26926)
                     /locus_tag="ACHKTW_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001056416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6463310.1"
                     /translation="MNMKKSSFLDEGKEPVIERIFRLAERYKSRSEAARAWGINIQTL
                     QNYYKRRNNQPPPQPRKHHLQNIAEKEHVSMEWLIYGYNNESITKKELLEQIERTKKQ
                     DVNDGDSIIMRAWDSLTSSEQEILSNLLIRKGAELLTILLDTDIQKLHSLSGVKRALA
                     LSLQELPDDTVREIYEECEAKVNHLNVTQKKAGA"
     gene            27093..27341
                     /locus_tag="ACHKTW_002299"
     CDS             27093..27341
                     /locus_tag="ACHKTW_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO6463311.1"
                     /translation="MSRREVSDHDWPPEIIKARLHMAGLSLRSLSLKAGYSRDSLKSV
                     LRTPCRPYQQIIADALGVSPEEIWPSRYQVKSYMRKAS"
     gene            27343..29433
                     /locus_tag="ACHKTW_002300"
     CDS             27343..29433
                     /locus_tag="ACHKTW_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu transposase C-terminal domain-containing
                     protein"
                     /protein_id="HFO6463312.1"
                     /translation="MYFIAKELIGAPGMPATTKGIRQALQRYAQGKSCCSRRRSGSKA
                     TEYSIDCLPEVTQQALRERYALQLMAQKADESPVPVVTKARRSPAVVDAVEAYRGSPQ
                     LMVERINALTEKQRQVADARIAIVSEVLKFAQQPGFSCAKAIRFIVDRLSRSQLDERI
                     VAMVETANAKKGNSRALSEITLKRWIAAFNKAQNAAERLLLLAPGKRQEIKAEDISWL
                     PEFLAQYRQSNGRPMTEAYEDFVAEWQHRHADEPYMLDIMPSYDTIRRAMKKLPEVVK
                     QKGRVTGSEYRQLEGFTRRDWSRMPVNYVWIGDGHGMKLKCAHPVHGRPFAPEVTFVI
                     DGGTRFVVGWSLDLAENVFAVAGAIQHGIRHHGKPFLYYSDNGSGETADILDKEVVGI
                     LPRLGINHPTGIAGNPQGRGIIERLNRTLPMRIARKYRTYFGKGADRETLRKTNRDLR
                     SAFSALQQGKRLNARQQSAMRDLPSWSELIDAIRDGVEWYNNRPHDELPIKPNGKHYS
                     PAEFRKKRLAEEDTEIEWLSDVELRDMFRPMVERPVRRCEIRWLNNIYYAPELRDEHG
                     RKVLISYDIHDAERITVRRPDGSVICEAVWDGNKREAFPVSAEYYKQQQRLKGMRKRA
                     EEKIRDAEDEVVNVLEHKPQEPWLENIYRPVGNTVTVQQPVADDEPDEEYERNFQRGL
                     QLLEAKLKENDPLA"
     gene            29517..30449
                     /locus_tag="ACHKTW_002301"
     CDS             29517..30449
                     /locus_tag="ACHKTW_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFO6463313.1"
                     /translation="MTDINDVIKTIDELIDGGVLTQYAIAREAGISDGTLSAFRKGKY
                     KGDNAAVAASLRSWYENWNKQSALPEPPQFVETQTVQELRALFQAVRLMGCINVIVGV
                     PGVGKTATARNYCQEQPNTWMITLSPAHSSVTECLLELAEELGIDYTRANKGALSRAI
                     RRRLMGTRGLVIVDEADHLGIDGLEQLRAIQDATGIGMVLIGNPRGLFKGGRRAVDDL
                     SRLFSRLARTKQLRKAKKADVLAIARAWGISGEAELAVMQAIAEKPGALRVLTHTLNQ
                     AWITASGEGAVLTEKHINAAFKEVYTNPELLSQV"
     gene            30452..30673
                     /locus_tag="ACHKTW_002302"
     CDS             30452..30673
                     /locus_tag="ACHKTW_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000257931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6463314.1"
                     /translation="MAVFNIPDIYGRFYLVNFDNVKVISLAENKECGDLLFEFNDRTR
                     MVISAGLDREGATEVYRGICRSVGAKQVR"
     gene            30686..30940
                     /locus_tag="ACHKTW_002303"
     CDS             30686..30940
                     /locus_tag="ACHKTW_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6463315.1"
                     /translation="MNMQSCGKKMNLFDSLNSARRLTELAGAVLERSKRYPQRYALKT
                     TPPVSEVKGTGEIEITIQTNGLRRRVKATRISGCTVYWEE"
     gene            30942..31223
                     /locus_tag="ACHKTW_002304"
     CDS             30942..31223
                     /locus_tag="ACHKTW_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host nuclease inhibitor protein"
                     /protein_id="HFO6463316.1"
                     /translation="MKRNLIAWAWSSGLIELGYVLPEGALPIVAGKPATVRHVIEVMA
                     RHGRDEQEQLLVPGIPEAVTEEEAFNAMIRFCREVRRRVSYPNRTRTRG"
     gene            31220..>31482
                     /locus_tag="ACHKTW_002305"
     CDS             31220..>31482
                     /locus_tag="ACHKTW_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6463317.1"
                     /translation="MSKVVRIIFEYKEHVIHKNADGTVRMGISLDIRSTGIKQKGDGP
                     AMIFGVVMLAESRDFAELVAMKASALMKDMSMRSGVIKGNEFN"
BASE COUNT         7523 a         8450 c         8024 g         7485 t
ORIGIN      
        1 tataaacatt taacatgata atattaaaaa cagactttat tatcgtaata aaaatcgccc
       61 aaacattcct gttcgggcga tcaaattaat tattgtttat cctgcatata cggcgtgtac
      121 accccgttca gactgtgcag gaaagctacg atatcatcca catcctcctg cggcagctct
      181 ttgcctacct gatagcgcag catcagtttc accgccccgt ccagcgtcgg cacgtcaccg
      241 cgatggaagt acggtgccgt taaagcaacg ttacgtaaac cgggtacttt ctgacgcaat
      301 ttatcacgct cttctttagt cacattcata cgaccaatat ccgccgccgt aatttcccca
      361 aagttaaagt cttttttcag ccccaacggt tcaaaggaac gtccgccgag aataatacca
      421 ccatgacaag ttgcacattt attatcttta aataattgat agcctttttt ctgttgcgcc
      481 gtcagcgcat tttcatctcc acgcaaccat ttatcaaatg gggaatccgg cgtaattaat
      541 gttttctcaa attcagcaat ggcatcagta atattttcgc cactgaaacc ttgcggataa
      601 acttcgagga actgcgcttt aagctgcgga tctttttcca gcttagcaat aatttcgtcc
      661 caggatttcg acgccatttc aatcgggttc aacggcggtc cacctgcctg atcctgcaat
      721 gttgccgcac gaccatccca gaactgctca acgttaaata ctgagttaaa taccgtcggc
      781 gcgttaatcg gcccaactgc gccaccaaca ccaatcgatg tttttctgcc atcgacgccc
      841 cccgcattca acgcatggca atgagcgcat gaaatggtgc tatcagccga taaacgggga
      901 tcgtgataca gcgcaaagcc caacgccact ttttgcgcat cggtaggcag tttttgcggg
      961 atgggctgca ccggttcatt gcgatgctcc ggagcagtat cattgctggc gtaatattcc
     1021 gcgcgctgtt ttgcaatcca ggccagtatt tccgcccgct cttcatcact caccttaccc
     1081 gcccagtgta gtgcggtata acgcgttggt ggcatagttt catactgcat cacccattca
     1141 atcttattca gatcgctttg cgaaacgggt ttatcagcca gcagtgccgc acgcacggcc
     1201 tcgaggttaa aagatttata tccaagctta atgtcgtaat ccatcaactg tttcgcgcca
     1261 ggaatataat aataggcggg taattctgcc gaaggtgtgt ggcaatagtc gcatcctttt
     1321 tcgcggagaa agcctaaaac cttattattt tcactgacag cagatgcctg aacatcggcc
     1381 tgtttactgc gtttattatc gtggtaccac acataaccag ataaccctaa atagcaaatc
     1441 gcgacgccag ccaggccgat cgcggtaata cgtgagacca ttttcattat tcttccctca
     1501 cggtggtgag ttattgtgac ccagaaaatt ttccagaatg ctgcaaacgt ctctgcgaga
     1561 caaagccatc ataaaaaaca gggagatacg ctttttgata ggaatcaatc aattacatga
     1621 atataaacta tcaataagat agcctgtgcc tattacccag atcaggaatg gcatatcatg
     1681 attgcggcga gagcaggatt gaatgttctg aaaatgaaaa acaacacgaa atccgtattt
     1741 ttataaattc catggatatt agtgcgttat attttttgac aggttaataa acgtgatcca
     1801 ccgcacgctt tgtcgcccac caggcggagc gaatgactac ccttaaagaa aagcccgata
     1861 attagcgacg aatttcggag gttggatcct tatgctcaat cagaaaattc aaaaccctaa
     1921 tccagacgaa ctgatgatcg aagtcgatct ctgctatgag ctggacccgt atgaattaaa
     1981 actggatgag atgatcgagg cagaaccgga acccgagatg attgaagggc tgcccgcctc
     2041 tgatgcgctg acgcctgccg atcgctatct cgaactgttc gagcatgttc agtcggcgaa
     2101 aattttcccc gacagtaaaa cctttcccga ctgcgcaccc aaaatggacc cgctggatat
     2161 tttaatccgc taccgtaaag tgcgccgtca tcgtgatttt gacttgcgca agtttgttga
     2221 aaatcacttc tggctgccgg aggtctactc cagcgagtat gtatcggacc cgcaaaattc
     2281 cctgaaagag catatcgacc agctgtggcc ggtgctaacc cgcgaaccac aggatcacat
     2341 tccgtggtct tctctgctgg cgctaccgca gtcatatatt gtcccgggcg gccgttttag
     2401 cgaaacctac tattgggatt cctatttcac catgctgggg ctggcggaaa gtggtcggga
     2461 agatttgctg aaatgcatgg ccgataactt cgcctggatg atcgaaaact atggtcacat
     2521 ccccaacggc aaccgcacct attatttgag ccgatcgcaa ccaccggttt ttgcgctgat
     2581 ggtggagttg tttgaagaag atggtgtacg cggtgcgcgc cgctatctcg accaccttaa
     2641 aatggaatat gccttctgga tggacggtgc agaatcgttg atccctaatc aggcctatcg
     2701 ccatgttgtg cggatgccgg acggatcgct gctcaaccgt tattgggacg atcgcgacac
     2761 gccgcgtgac gaatcctggc ttgaggacgt tgaaaccgcg aaacattctg gtcgcccgcc
     2821 caacgaggtg taccgcgatt tacgcgcggg agcggcctca ggttgggatt actcttcccg
     2881 ttggctgcgt gatactggtc gtctggcgag cattcgtacc acccagttca tccccatcga
     2941 tctgaatgcc ttcctgttta aactggagag cgccatcgcc aacatctcgg cgctgaaagg
     3001 cgagaaagag acagaagcgc tgttccgcca gaaggccagt gcccgtcgcg atgcggtaaa
     3061 ccgttacctc tgggatgatg aaaacggcgt ctaccgcgat tacgactggc gacgcgaaca
     3121 actggcgctg ttttccgctg ccgccattgt gccgctctat gtcggcatgg cgaaccatga
     3181 acaggccgat cgtctggcaa acgccgtacg cagccggtta ctgacacctg gcgggattct
     3241 ggcaagcgag tacgaaaccg gtgaacagtg ggataaaccc aatggctggg caccgttaca
     3301 atggatggca attcagggat ttaaaatgta tggcgatgac cttctgggtg atgaaatcgc
     3361 gcgcagctgg ctgaaaacgg tgaatcagtt ctatctggaa cagcacaaaa tgatcgagaa
     3421 ataccatatt gccgatggtg ttccccgcga aggcggcggt ggcgagtatc cgttgcagga
     3481 tgggtttggc tggactaacg gtgtggtacg ccgtttaatt ggtttgtacg gcgaaccata
     3541 atatttttac agccagccgc taacttcctg ctggctgtaa aattatcctc ttcaggagga
     3601 gatatttaac atcattgccg cctgggtgcg atttttcact tccagacggc gatacaggga
     3661 ttccagatgc gcttttaccg ttccggtact gatattcagt gctctgccga tctccttatt
     3721 tgattcgccc gccgctaaca tggttaaaat ctcccgctgg cgggcgctta acgatttgag
     3781 atctttaatg tccttttccg gcgtcgtccg ccagtctcca ggcagaaaca tcatccccat
     3841 cgccgcacta tttaccgcca acgcaaatgt ctcgacggtt gaatcacgag gcacaatggc
     3901 cagcacatta aaatggataa cttcctgtaa ccaccgttta ttgcaatccg tcgccgtgat
     3961 taacacctta acctcaggaa actgcaccac ggttttttgc agcaaccagt agcaaaactc
     4021 accatcctga tcgccatcga gcataactaa ggcttcaggg taactttcca gcttttgcca
     4081 taactcgtct gcctgactgg ccccctgaat actcactcct ggaatacgct gctgtaaact
     4141 gattttcatt ccatgaataa atattgactg cctgtcaaac atgactattt gcataactga
     4201 atctccacct gaatacgtta aaaagactta agtagtggaa gggtattacc cgcgagaaaa
     4261 aataagaatt cgccatttgg cggtggccat tctacagaga tgacgcgtag aaaatagtta
     4321 ccgatataaa tagttacagc taaacgcctg aaattacatg tcgagggcac tatttaaaac
     4381 aattttgagg atttccttat attggtggtt agtacgcatg caattaaaaa tgaaactccg
     4441 cgaccacaag caaaaataac aaacggcaat gagacaaaaa taagcacaaa tagccaacac
     4501 gtcctctatt cactttaaag ggaatcgctg aaaaatacgc tctgtttagg gggattcacc
     4561 tttctcagaa agctattccg cccttttcct gctgagaaat cgccacattc gtcatgacaa
     4621 cattgtgaaa cccggcatta gatgttagaa aaaacaataa acaatgcgat atgcgcgtta
     4681 ctgtttcgct gtgaacaaca atcggtcaaa gaaatggata aaattcacgc aatgcagttg
     4741 ttcatcaaag tcgcggagct ggaaagtttt tcccgcgcgg cggatttctt tgctttgcca
     4801 aaaggaagtg tttcgcgcca gatacaggca ctggaacatc aacttggcac ccagcttctc
     4861 cagcgcacca cgcgacgggt caaactcacg ccagaaggca tgacctatta tcaacgagca
     4921 aaagatgtgt tgagtaatct cagcgaactg gacggtctgt ttcaacagga tgccaccagt
     4981 atcagcggta aattacgcat cgacatcccg ccaggaatcg cgaaaagcct gttactgccg
     5041 cgcctgtcgg aatttctcta tctgcatccg ggaattgagc tggaactgag tagccatgac
     5101 cgtccggtag atattcttca tgatggtttt gattgcgtga tacgcactgg cgcgttaccg
     5161 gaagatggcg ttatcgcccc tcccctcggc aaactgacca tggtcaactg tgccagtccg
     5221 cactatctga cgcgctttgg ttatccgcaa agccccgatg atctgacttc acacgcaata
     5281 gtgcgttata caccgcacct gggtgtacat ccgttaggtt ttgaggttgc cagcgttaat
     5341 ggcgtccagt ggtttaagtc tggcggcatg ttgacggtaa acagtagcga aaactatctc
     5401 gccgccggtc ttgccggtct ggggattatt cagatcccgc gcattgccgt gcgcgaagcc
     5461 ctgcgtgccg ggcggcttat tgaagtgttg ccaggctacc gtgccgagcc gctctccctt
     5521 tcgctggttt acccgcagcg tcgggagctt tcccggcgtg taaacctgtt tatgcagtgg
     5581 ctggctggcg taatgaaaga gcacctggac tgaccgacta tactttttaa gaatgaccac
     5641 gacagaagga caaaagagcg gatgacgcag gaaaacgaga tcaaacgtcc cacccaggat
     5701 ctggagcacg agccgattaa acagttggat aatagcgaga aaggcggcaa agttagccag
     5761 gcgctggaaa ccgtcaccac cactgccgaa aaagtccaac gccaaccggt tattgcgcac
     5821 ctgattcgtg cgacagaacg ctttaacgat cggctgggta accagtttgg tgcggctatc
     5881 acctatttct catttttgtc gatgatcccg attttgatgg tgtcgtttgc cgcagggggc
     5941 tttgtgctgg cctcccatcc gatgttgcta caggatatct tcgacaaaat tctgcaaaac
     6001 atcagcgatc cgacgctagc cgccacgttg aaaaacacca tcaacaccgc cgttcagcag
     6061 cgtacgactg tagggcttgt cggcctggcg gtggcacttt attccggcat caactggatg
     6121 ggtaacctgc gtgaagcgat tcgtgcccag tcgcgcgatg tctgggaacg ctcgccgcaa
     6181 gatcaggaga agttctgggt aaaatatctg cgtgatttta tttcactgat tggtttgtta
     6241 attgcgctga ttgtgacgct gtcgatcacc tcggttgccg gttcggcgca gcaaatgatt
     6301 attagcgccc tgcacctgaa cagcattgag tggctgaaac cgacgtggcg gttgattggc
     6361 ctggcgattt ccatcttcgc caactatctg cttttcttct ggatcttctg gcggttgccg
     6421 cgccaccgtc cgcgcaaaaa agccctgatt cgcggaacat ttctggctgc tattggtttt
     6481 gaagtgatta aaatcgtgat gacctacacc ttgccatcgc tgatgaaatc cccctctggc
     6541 gcagcatttg gttccgtgct ggggctgatg gcatttttct acttcttcgc ccgtttgacg
     6601 ctgttttgcg cggcgtggat tgccaccgcc gaatataaag acgacccgcg aatgccaggg
     6661 aaaacgcagc cttaaaatag gccggatgcg gcgtaaacgc cttatccggc atacgatttt
     6721 attcggcctg gctccccgta ggccggataa gatgcgccag catcgcatcc ggctataatg
     6781 cgcacataac ctcttgaaac tcttccccag agcctcttca gccatctatt tgggagcaaa
     6841 caatttcatt ccaactcata accccagcat ataaatccag ttggtaactt ttatttaacc
     6901 tgaaaccagt tttatccact atttataaaa ttatgtgaag catttcatag aagaaaaatc
     6961 actggcctaa acattatccc ctttttgcct ggtttttgac catttccgcg atttgttaca
     7021 cattgaaata tcacttttgc tgtgcgtaat atggctattc gttagccaaa aaataagaaa
     7081 agattatgca agcaacagcc acaacactcg accacgagca agaatacacg ccgatcaact
     7141 cgcgtaataa agtccttgtc gcctctctca ttggcacggc cattgagttc ttcgactttt
     7201 acatttacgc cactgcggcc gttattgtgt ttccgcatat cttcttcccg cagggcgatc
     7261 caacggcagc aacgctacag tcgctcgcca ccttcgccat cgccttcgtc gcgcgcccca
     7321 ttggctctgc cgtttttggt cattttggcg atcgcgttgg gcgtaaagcg acgctggtcg
     7381 cctcgttgct aacgatgggg atttcgaccg tggtgattgg tctgctgccg ggctatgcca
     7441 cgattggtat tttcgccccg ctgctgctgg cgctggctcg atttggtcag ggtctgggct
     7501 taggcggtga atggggcggc gcggcgctgc tggcgactga aaacgcccca ccgcgcaaac
     7561 gtgcactgta tggctccttc ccacagctgg gcgcaccgat tggcttcttc tttgccaacg
     7621 gcactttctt gctgctttcc tggctactga ccgacgagca gtttatgagc tggggctggc
     7681 gtgtgccatt tatcttctcg gcggtgctgg tcattatcgg cctgtatgtt cgcgtgtcgc
     7741 tgcatgagtc gccggtgttt gagaaagtcg ctaaagcgaa aaaacaggtg aagatcccgc
     7801 tgggtacgct gctgaccaaa catgttcgcg taaccgtact gggtacgttc attatgctgg
     7861 caacctatac gctgttttac atcatgacgg tctactctat gaccttcagt accgccgccg
     7921 cgccagttgg gcttggcctg ccgcgtaacg aagtgttgtg gatgctgatg atggcagtta
     7981 ttggttttgg cgtgatggtg ccagtcgccg gattactggc tgatgccttt ggtcgccgta
     8041 aaagcatggt aatcatcacc acgctgatca tcctgttcgc gctgttcgcc tttaacccgc
     8101 tgctcggttc tggcaacccg attctggttt ttgccttcct gctgctgggg ttaagtctga
     8161 tgggactgac cttcgggccg atgggcgcgc tgttaccaga gctgttcccg acagaagtgc
     8221 gttacaccgg agcatcgttc tcttacaacg tagcgtcgat tctcggggct tccgttgcac
     8281 catatatcgc agcctggttg caggctaact acgggttagg tgcggtgggg ttatatctgg
     8341 cggcgatggc tggcttgacg ttaatcgccc tgctgctgac ccatgagacg cgacatcagt
     8401 cgttgtaagt atcgtgtggg cagccaccct caccccagcc ctctccctga aagggagagg
     8461 gagaaaagcg gagcgagatg ttatatcgcg aacataacag ccctccccca gaaagtgaac
     8521 ggaaaagcgg tactcgttgt ttcaacacgc acaaaacagt cccctcgccc ctttggggag
     8581 agggttaggg tgaggggaac acgctgctcc cgaactcacc agcaaacaca atccaacctc
     8641 atcacttctt catctgcgac aatatcgtcc gacactgatt agcctcccct tcggaaggtg
     8701 agatcagtgc cagtaacgcc gccgccggtg taaccagcgt tgccagtgcc gcagcaacag
     8761 caccacgggc aatcaacggc ccggctttca ccccagcctg cggattttta aacgtcccac
     8821 gcacatacag cggcgaacgc agtgtgataa tccgaattcc tttactctcc ggatcaatag
     8881 tcaaatccag ctgttcggaa gcaaaacttg ccgtgccggt aacgttaatc aacgcgttct
     8941 cagtatcgaa agcaaagatc tgcgggcgcg ccacgccgtt ggcaatatcc agattcgccg
     9001 ccgcgcagtt cacccgcacc tcatcatcac caaatatcgc accgacaatg tagttgccga
     9061 cattcagccc aacaatctcc atcaggttgc ggctcaccag cccgtcattc atcaacagtt
     9121 tcaggttgcc gttactgttg cctaaaagtg ccgccaccga gttaccgcta ccgcgcagtt
     9181 ccgcgtcacc gttcatttcc cccagcgtct tctgcatcag ttccacatcg ggcatcagtt
     9241 ctttcagttt cagtcgacga gcctgaatat ctgcccgccc ctgcatcggc tttttatcgc
     9301 cttccagatg aatattcgcc gcaatgctgc cacccgccat gccaaatttc agcggttgca
     9361 ggcgcaggtc agcatttttg aggatgatat gagtagaaag atcgctaatc ggcaggctac
     9421 tgccatgctc aatgcgccgc cctttgaagc gaacatcggc atccataacg tcccatttat
     9481 cggtttcgaa gcggtcataa ggcagcactt tgcccgcagg ctgaacgctt ttttcgccct
     9541 tcttctgttc agaccgtttc gacttttcag cccctttccc ggaatcaacg ccaatcaacg
     9601 gtcccaggtc cgccagccgc aattgccgcg actcgacatc accttccagt tttggtcgtg
     9661 gcttgccggt ggtgtagatc agagaaccgt ggatatcgct atcgccaatg cgcccattaa
     9721 aaccgcgata atcaaagacc gacgattttt cagtgtcgat tttcgctacc agccgaccat
     9781 ctgtttcaaa cggcggggta tcgggcagca gaacgcccgt cagttcatag agatcaccca
     9841 gtgaatcgcc agaaaattta agccgtaaat cgacaccgcc catcttcatt gggtcattca
     9901 cgacgccatc aaaagcaaca cgggtattac ctgaacggaa atcagcctgt accggaaacg
     9961 gcgtcccttc gccacgcagc gccagcatac cgcctatttt tcccgtacca gtgagcggtt
    10021 caccgttata acgtccctgc gccttcaggc caaaaacgta atcgcccacc ttttctttat
    10081 ccgctttacc tttcgatcca gtaacttcgc tgaacggcag cggcttacct aacggatcga
    10141 caaaaatttc cagatccgct ttgctcactt tgtcatcaat ggcgatccgc ccttgatcga
    10201 aaagaatatt atccagccga aacgaccatg ccgacggctt tgcattcgcg tctttgttat
    10261 catcgttggc gagattaaac gtccagttat tgttcttttc agagaggcga atcaggcgcg
    10321 cgtcgggctt ttcgagcttg atccacggca gccagaccgt tttggtcagc agcgccagcg
    10381 gggccagcgt tgcctctacg cgtggcaaat gcaccatcgt gacttcggga atatccggtg
    10441 gattgccaag aatgatgtct tccgcgtgta catggggcca tggcacccag ctgcgccagc
    10501 cagtttcttg tttttgccgc tcccacacca cgcccagatc gccacggata gcgaacggac
    10561 gattcaactc cgcagagact ttctggttga tggtcggttt gagtcgattc cagtcaaaag
    10621 ttgcaatcaa aatgatcgcc acgacaatca acaacaagaa agcccctgaa atcgcagcgg
    10681 ttattttgcc tgccttgctc atcgttcgct tcctcatatt cttcctgtgc cagtcctaaa
    10741 gatagtccag ccaggcggaa aatgaggcag cttatagcgc cagaaccgcc gtattcagcg
    10801 tttctatcgg tgccgggcgt gaaagaaacc agccttgtgc ggcgaatgcg ggcgagttct
    10861 gaacatcacg ccactcttcc ggtgtttcta cgccctcgac aatcaccccg cgacaatagc
    10921 gattcattag atgtaaaagc tgagaaaaga gtgtgcggcc ttccggcgac tgacgcagca
    10981 tcacaaacag ttctcgcgcg attttgatgt agtcataacg cacttcactt agtgcagaga
    11041 aatttgccat cccggtacca aaatcatcca gccacagcgg gccaaattca cacatcgagg
    11101 caaaggttga atctttcggc agacggatat gctccaccag ttcgaaacgc agccagggaa
    11161 gacgctcaat ctggcgcagg atttttggtt gctgacgcag ggcgatgagc gtagggccat
    11221 caatattgac cgatgccagc aggccgtgct ctataaagaa gtcggctttt tgcgccagca
    11281 aatcaatctg ctctttcaca acctccatcc gatggctgac ggtgatttca gtaaaatagc
    11341 gatccggcgg caggcgttgc gaagggttca agggatgcgt gaccaccgtt aatagctcca
    11401 cggccattaa ccgcccgcat gtttgataga tcggctgcca ggtgtaagca cgctcacact
    11461 gcaaccaaaa acgccgttcc tgcaagctct cgatgcttgc ttcagggttg cttattcgct
    11521 ggataacctg ccttatcatc aaagatgtcc tgtgtttaaa agtgatgctg cccggactcg
    11581 acaaagatta tcggcgcgca agggcagaac tttaccgcct ggccggggta aatttcccct
    11641 ctccacaacg gaatctttca ggaatacgtt ctggctcaaa aaaataatgg aacactgttt
    11701 taatatggtt gaccagcaaa ccaccacagc gcaaactaac gctaattttt tacagatcag
    11761 gttcacgact atgtccaaaa agattgccgt gattggcgaa tgcatgattg agctttccga
    11821 gaaaggcgcg gacgttaagc gcggtttcgg cggcgataca ctgaatactt ccgtctatat
    11881 cgcccgtcag gtcgatcctg cggcattaac cgttcattac gtaacggcgc tgggaacgga
    11941 cagttttagc cagcagatgc tggacgcctg gcacggcgag aacgttgata cttccctgac
    12001 ccaacggatg gaaaaccgtc ttccgggcct ttactacatt gaaaccgaca gcaccggcga
    12061 acgtacgttc tactactggc gtaacgaagc cgccgccaaa ttctggctgg agagcgagca
    12121 gtctgcggcg atttgcgaag agctggcgaa tttcgattat ctctacctga gcgggattag
    12181 cctggcgatc ttaagcccga ccagccgcga aaagctgctt tccctgctgc gcgaatgccg
    12241 cgccaacggc ggaaaagtga ttttcgacaa taactatcgt ccgcgcctgt gggccagcaa
    12301 agaagagaca cagcaggtgt accaacaaat gctggaatgc acggatatcg ccttcctgac
    12361 gctggacgat gaagacgcgc tgtggggtca acagccggtg gaagacgtca ttgcgcgcac
    12421 ccataacgcg ggcgtgaaag aagtggtggt gaaacgcggg gcggattctt gcctggtgtc
    12481 cattgctggc gaagggttag tggatgttcc ggcggtgaaa ctgccgaaag aaaaagtgat
    12541 cgataccacc gcagctggcg actctttcag tgccggttat ctggcggtac gtttgacggg
    12601 cggcagtgcg gaaaacgcag cgaaacgcgg gcacctgacc gcaagtaccg ttattcagta
    12661 tcgcggcgcg attatcccgc gtgaggcgat gccagcgtaa acgcagcata tgcaaaaggc
    12721 tggataaagt gttacccctc tatccagctc atccataaat acctgatggt tgtgctaact
    12781 catcaccatc aggcattact gtgcaggtgg aatatccgtc acttcaggat gtacgtcatc
    12841 cgtggcgaca gaggtggcgg cagcagcagt cgatggggcc atgatttgat cccaggccgc
    12901 ctgcaacgct ttcatgttaa attccggctc gcctttcggc tgcagcagta ttaacgccat
    12961 atcattcgac aactgctgac gcagatcctg atttaacatc tccaccgtca ggctgttaag
    13021 gaaatcctgc cgcagtttct gatactgctc cggcgcgata tcgacgacct gattttgcaa
    13081 cgaacgcatc cgctgaccca tcagaatatc ggtatcagcc cgcgcatagg cggcaaacag
    13141 tttctgcagc tccagtttct tttgcgccac taacgcattg aactcttctt ccggcagacc
    13201 tttatcgcga accttcgcca gttcacgcgc caccagatta aggttgctgt tcagcttgtc
    13261 gtttggtgat tcgatgttga tggcacactg cgcacgcaga tacagcacac ggcagtcaaa
    13321 tccaagaccg atgtctttgc tgttactggc gcttaacgct tgctgaacat gccagaacag
    13381 cgcctcgcgg gccaggtccg cacgccagta gcgcagcagt gcggctgatt cacgaatcgg
    13441 ctgccacggc gtatcccaca tgatagataa ccggtcctga cgcaccgcgt cagtcataat
    13501 actcaccgct tccgcacgca gcggagaaag cgtcggcacc ggagctggcg tttcacgttt
    13561 gcctttcagt tcgccaaacg ttttgtttat ttggtcgaca accgagcgcg catccacgtt
    13621 tcccaccacc agtagcgtca ttgcatccgg ggtgtaccat ttctgataga aatctttaat
    13681 tttttccgct tcaacgggtt gtttcagcgg atcggcagga tcgtgaccta acaaggttga
    13741 ccctttcaga cgatagcgcc accagccctc tttagtatcg gcaggccagg ttgccaccat
    13801 gtcctgactt tgcagcgcgt ggttgatggt ttctggtgtg atggtcaatt tgccagtggc
    13861 atttgccaga taagagagcg cttctttcag caaatcgtta cggttattgg gcaaactcag
    13921 attaaacagc gtggtgtcat aagagacaat taccggcggc atcgggcgtt tagggtcgat
    13981 cccctgctgc cacaatgaac gcgcctgtgc tgcgtcaagg ccaccgcttt gcgttagcgc
    14041 aatacgaggg atggcgtgac tgtaaccgct ctgttgtgta ctatcggcga gcgaaccggt
    14101 attaaccagc aggcgaattt caacacgatc gctgggacgc tggggggtgg tcagcacttg
    14161 ccactgtaaa ccgttggaaa gcgtcccctg ttgccatgct ggatcaggct ggagcgcatc
    14221 tgcctgcaca tagccagcag tggccatcat cagcaaaccg cccgctaaaa gtcgaatttt
    14281 tgtgccctgc atgtgaaccc ctgatcaact atcctggtaa taaaaaaact gcccgttgtc
    14341 ggtcagtcta aatgacgtta aaaacacttc gtgttagacc gcaagaacat gaaaatgtca
    14401 cggaagaagt gaaataaacc tgaactcacc caggtggtaa gttcaggcac aggggtcaat
    14461 tatgcgcaaa cacccgcact cggggaaggg agtgcgggca taagtgatga gattaagagg
    14521 ataattcgtg cgttttgcca tccggcgcac gattattcag cacatcgtcc agttttttgt
    14581 ggtccagttc tttcacccac ttagcaacga caatggtcgc tacgccgtta ccgaccaggt
    14641 tagtcagcgc acgagcttct gacataaagc ggtcgatacc gaggatcagc gccagacccg
    14701 ctaccggcaa atggcccacc gcagagagcg tcgccgccag cacgataaag ccactacccg
    14761 ttacccctgc cgccccttta gaagaaagca gcaacacgat taacagcgtg atttggtgga
    14821 cgatatccat ctgactgtta gtggcctggg cgataaacac cgccgccatt gtcaggtata
    14881 tcgatgtgcc atcaaggtta aacgagtagc ctgtcgggat gaccagcccc accaccgatt
    14941 tgcggcagcc gagtttctcc atcttgtcga gcatacgcgg cagcgccgac tcggaagatg
    15001 aagtccccag tacaatcagc agttcttcac ggatgtagcg gataaatttg aagatactga
    15061 aaccagtcgc tttagcgatt gaacccaata ccagcaccac aaacaggata caggtaatgt
    15121 agaaacagat aatcagctgc cccagttgca ccagtgtgcc gacgccgtat ttaccgatgg
    15181 taaacgccat tgccccgaac gcaccaatag gcgccagacg catgatcata ttgatgatgc
    15241 cgaagatgac ctgcgagaaa ctttcgatga cgttaaaaat cagttggcct ttgctgccca
    15301 gacggtggag cgcaaaacca aacagtacgg caaacagcag cacctgcaga atgttaccgc
    15361 tggcaaatgc gccaatgacg ctcgccggga tgacatccat aatgaaggcg acaatgccct
    15421 ggtctttcgc ctgatcggcg taaaccgcta ccgctttcgc atcaagcgtt gccggatcga
    15481 cgttcattcc ggcaccaggc tgcacgacgt taacgatgat aagaccaata atcagcgcga
    15541 tggtactgac aatttcaaag taaagcagtg cgactgcgcc ggtacgaccg accgccttca
    15601 tgctttccat gcccgcaatg cccgttacga cggtacaaaa gatgacagga gcgatgatca
    15661 tcttaatgag cttaacgaag ccgtcgccaa gcggtttcat ttgctcgcct atttcaggat
    15721 agaaatggcc aaggagaata ccaatggcta tcgctgtcag gacctgaaag taaaggcttt
    15781 taaacagaga ggttttcata gggtgtcctt tagtaaaacc acaggtcttg taaggttatg
    15841 gggtacctgc ggccttaaaa taacacccag acaacatcac agaaatgtac ctggatcata
    15901 attgaaacaa aaaggttaaa aagtttgagc tggctcgcac aaaccagcac tttttaaagt
    15961 tttgtaatca gtttggggta gctacttttc ttccaggtaa ctctcttcga agatttcaat
    16021 agggagtggg cgagcaaaaa ggaagccctg ggcaatacca acgcccgctt ttgccagcca
    16081 gtcgcgttgt gcttcagtct ccacgccttc ggcaatcatt tgtaagttca ggctctgcgc
    16141 cagcatgata attgcagcaa tcatgctgct atcttccggc aagccttcaa caaacatttt
    16201 gtcgattttc agtacgtcga ttggcaacga tttcatatgc tgcagctgac gcagccctgc
    16261 gtagcccatg ccgaaatcat ccagcgccac ccgaactccg gcattgcgca gcggacggag
    16321 gattgccacc gcagcatgag ggtcgtcaat acgtcggctt tctgtcactt ccagaatcag
    16381 tgttcccggc tgaatgcgat agcgggttaa cagttccagc atatccgcca ccatatttgg
    16441 gtgcatcagt tgcagcgcag agaggtttac cgacaagggc agcataatgc cgcgctcttg
    16501 ccaggctgca agcaatcgac aggactcttc cagcacccag tgaccgacgg taaccatcag
    16561 cccacagcac tcaatgcgat cgattaagcc atccggcagg tcccaactgc catccggttg
    16621 ctggatacgc agtaacactt ccgcactgac cagttttccg ctggtcatct cgacctgtgg
    16681 ctgtaaccaa atagcaaact gatgattttc cagtgcatta aggatatcgc tctcttccgt
    16741 caaccgcttc tgggcggctt ccatctgctg cggatcaaag aactgaatct gattcttgcc
    16801 tttatggcga gcggtaaatg ccgcagaaat agcgcgactg taaagctgtt cggcggtgag
    16861 atcgccgtag aacatcgcca cgccaatgct acagtgcgga cggagttgaa tacgttcaat
    16921 cggcaggcgc tcgctcatga tagtgagcac ttgctgacct aaggtgattg cgtgccacgg
    16981 ttcctgtaca ccgttggcaa tgacagcaaa gtcataaccg ctaatctgcg cgaggatcat
    17041 acgtggcgac agtaccgatt tgagtttttc caccagcgtc agcagcagaa tttctcgttg
    17101 cgcctctttc agcacgcccg cagtatcacg cagggtttca caggtgatga tcatcagcgc
    17161 ggtggtttgt ttacgcgcga caacctgctc cagcatctcc atcagcaagg ctttgttcgg
    17221 caaatccgac accgggaagc gcatcgcatt ttcgttctgt tcttcataat ggcgctgcag
    17281 caattgctgg ttgaggttgt aactgcgcac caacataccg atttcatcgt cctgatgcag
    17341 acgcggtaat gccagttggt gaccaacaag ctcctgggct gggatggcgt taagttcgcg
    17401 agcaatattg cgtaacggat gcaaaatcag gcggttaatg caccagctga tggcgacggt
    17461 caggataagc gacaaaagta agtaaatggt cactaacgtt gagagggtgc tcatcacgaa
    17521 cttatacata cggaaggaat ccgcctgtag taccagatag gcaattggct gcgggtttgc
    17581 cggacgttcg agcgagtaaa cgcccagcga gatttgaacc ggtagctcaa acaggcgagt
    17641 aaccattacc ggcaccgggc gctctggaat aaaactttta cgcagcgcct ggaactggtt
    17701 aggcagcact acatcggcac ggctgaccac gcctgccggt ttaatgctgg caagaatagc
    17761 ttccgcttcg ggaatatcgc ctttcaaaat agctgaagat aagggttcgc ggacagagcg
    17821 agcaatgctt tccagttgcg tagccgtgtt atagcgattc tgttggacca gatggaacag
    17881 caaaacggtg caaaaaataa aaacgaacac caggacaacg gctgccacca ttgccatctg
    17941 cttgattgtt aacgagcgac ttacgcgcaa attgtctccc cccggaaatc ccagattgcc
    18001 gcccgagtat acccgatcgc agcggcatta agagaggcgc tatctgaaaa cttaccagtc
    18061 ggcgtaaggt atcagcggct gcggcggtaa atccatgtca ccctgccatc cggcggcgga
    18121 ataacgtacg tagagcagga aatggctggg tgcgtaatct ttcgcctgct ggatatcaat
    18181 tgccgtgcca acaaaccagt tggaagtaac acgtcgttca agtaatgccc gcgccgtgta
    18241 gccgaagccc tgactgctgc cgccatcgtt ggattgccgc gcagcttctt cctgccagtc
    18301 ggtcgggatc agattcatca gcggataacg cggcatggtt ttggtgcgtg aatgcgacca
    18361 cgagccagac gctcccagct cccacgacca gttttccgtg cgctcccgcc acatcaccgg
    18421 tatggcaaac gacaggtatt cctgcggact gtagtaaccg ccctgaccga gtgagtagcc
    18481 actcagatct ttgtcgtaat gccagatcat gttgttcagg ccgattgtga cgcggcgatt
    18541 gttctggtta atgaccttat agtaatagcc cgtcatccag cgcacgcgcc agttatcttc
    18601 gacattttta ccggttaact ggtcgccact aagcgatgcc cagacgccgt ttgcttcacc
    18661 tttatcgtag ctcagactta gccccacacc gtcggcacgt acgccacccc attttttccc
    18721 ggtattgctc ggggagtctt tttgcccacc aaaggccagc aaagaactgg agatgggccg
    18781 acggtgggcg ttaacggtgt aacccagcgg cccgatatca tcgctgtaac tgatgccgcc
    18841 gaccacatcc accacgttga agcccatcgg cgtggtaccg atatcccagc tccagacgtc
    18901 atttcgccag ccgaccgcca cgctggcacc ggaatccgac tggctgcggt tgccgctaca
    18961 gtcctgtaat gtacaggtgc cccagttgtc atcccatttg ccatcggcat tagtggagaa
    19021 actgccgacg ttcatattga cgaaatcact gcgaaagaac atccgcccgt cagaatacgg
    19081 cgcatccacc tgcaacatgg tagtgtgcgc tttcagatcg gagtaaccac cggtgccgct
    19141 cgaaccccag taatcgtgct caagggtgac gttaagatcc tgctggcgat agaggtccgc
    19201 cgcatcgctg cgcacgccgc gtttcagcca gtcatctttc tcgtcgttac gggtcagtcg
    19261 ggtgaatgtg tcgttatcct gcggacgcgt cgtagtcaca ccggatgcga ccatggcgtc
    19321 tttgtaggtt tccagcgcct gctttggatc gcccgcctgc gcttcaaact tcgcaccgtc
    19381 gcgcagcacc atcgcgcttt ccatcgacgg tggctgagat tttgcctgcg ggatcaactt
    19441 attaaacgtc cgctgcgctg ctgcggtatc gccaagctgc gcctgtgcca gcgccacgcg
    19501 ccgctgtgtg ttcagcgagg cgttatcggt agcgggcagt ttcgccagct ggctacgtgc
    19561 cgccgctttg tcaccggcag caatatccac ttccgtcaga ccaagaatgg cgtcggcgtt
    19621 agctggctcc cgcgtcagga cattctgata tgcagcacgg gcggcggtgt aatcacgtcg
    19681 ttgttgcgcc cagtcagcca gcgtgaggtc aatacgcgtg gaaggtggtt gctgacgcag
    19741 catcgcttcc gcttcagctt ctttgccgct ttctcgcagg cgattagcgg tttccagcac
    19801 ctgatcgctt tgcagtcgat taaccagctc ctgaatattg ctgttccact gcgcgcgcgg
    19861 caggctgttg atatgcgcca gcgccgctct gtcctggtca tgaccagaga ggtacagccc
    19921 gtaagcgtaa acctgctccg ggtcgttcgg cttatgctgc gccagattgc gcattaacgt
    19981 atcggcctgg ctgcgttgtc cggcctgcca gagatcctgc gaaagtcggt aagtaatcca
    20041 tacgctgccg gggtccagcg ccagtcgttg ccgctgaagt gctgccgcct gcgcccattt
    20101 gccctggttt tccagtgcct ctgcctgctg tgccagacgg tcgttttgca ggctgcgttc
    20161 gatatcatca atgctacgcc gctgactggc agagagcgag gcgataaacg cttcagcttt
    20221 ttctggcgat tgctggcggt aaatatttgc cagcccgcgc acggcgttag tgttgccgct
    20281 gtccatacgc aaggtctgct gataataacg ttcggcggcg ggataatctt ttcgcgccat
    20341 cgccacatcg cccagcccca gcactgcata actgtcggtg ttatcgacat tacgcgcctg
    20401 ctggaacagg cgttctgccc ggtcaggatt attggctttc agcgcagcat cgccctgctg
    20461 gatcgccagc cagtagcggt ttactttcag cagactgttc catttgtcgt tgttgctgct
    20521 gtgcgggtcc agtgcgaggg ctttttccag attcgccact gcattggcgc gatcgccttt
    20581 ctgagaatac gcctggccca gcgcccccag agcttcactg tctttcgggt tcgcccgcac
    20641 cgcctgttgt agttcgggaa tggctttacc cgccatacca gagtccaccg ccgctaaacc
    20701 ttgcgcacga gcgcggaaag caggatcggc cagctgtttt tgctgttctg ccagttgcga
    20761 ttgcgcagcc gccacgctat cgccatcact aaagatcgag agatattttt tcagcgccga
    20821 cacactggca tcactgacgg gcatgtcttt aatctgcccg taccagattt tagaggcccc
    20881 ttcgcgcccg gcgttcgatt ttgccatctg ttccaggacg gcaaaacctt cgtcacggcg
    20941 atcgctacta aacagcaata gcgccagatt gttttgcagg cccgtattgc ccggtgcatc
    21001 cgcattgatg cgttttaact gattaatcgc ttcgccacgg cgagccggaa ttttcgccac
    21061 cgtactccag tactcgacgg caatgtcacc ttccggcggc gcaccgttga acagtttgtt
    21121 gtaactcgcc acagcttctt ctgcatgacc ggtcgtcgcc tgcaatcgtg cctgttgcag
    21181 tgcctgacga ccatccggcg tggaaagtag catcgtagtc cgcgacgatt tatacgcatt
    21241 tgaactcggc gctaactgcg acagccgatc gagctgtttt tgcgcgccat caatatcgcc
    21301 ctgacgtaac aaagaacgga aacgggcggc aacgacgtcc gggttattcg gatcaataag
    21361 ttccagccga tataacgact gttgcaccag atcttcacga tgggtcgctt cgcctaaccg
    21421 aacttgctcc agcaactgtt gctgagcggt tggtgctgcc tcgaccatcg gcatgacggc
    21481 cagaccgagg gaaagcgtga atatgtttag tgtgaatttg cgcattcctg gccccagtca
    21541 ggtaataact caccttttgt cgagaagcgg aaacggtgtt gatcccagcc ttgtccaaac
    21601 agggtcagca catagttgta ataggcatcg ctgctgggaa agttatcggc cacgcgctgg
    21661 cgctgaacgg cctgcgcatc gcggttttgt aaaaagggca gcatggcggc agaaaaaccg
    21721 accggtcctt taccctgcgc tttccccgta gccacatcca ctttttccgg cggataaccg
    21781 tttttctcag tgaatgtcgc catcggttta aaccggttga gcatccgcgc tttttgcgga
    21841 tcgctgtcag gcatcatgcc tacccacatg taaacgcgga tagcgtcgta gctgctgatc
    21901 aatgtttttt cggcttttag ctgccagcct ttgtctttct catagcgcac ccagtctggc
    21961 gaaaaacctt tcggggcggt ttccagcaat aaacgttgat tggtttcgcg cagcgtggtc
    22021 cacggcgcgc caaagcgggt gaaatactgc gccagcgtcg gcggcaggta gctggggtta
    22081 aaacgccagc tgttatcctc agcaaaaccc actttgcccg gtaacaacat ggaacccagc
    22141 ccaggcaccg tcaccacttc ctcccgcgcg atacgtttta gcaacgcgct gccgatgtcg
    22201 gtataacgct gctctttcca caaacgcccc gcctccagca acgaccaggc catccagaca
    22261 tcaccatcgg aggccgaatt gctgtccagc acttcccact tactgttctc tttcttgccc
    22321 cacagccagg cgggcaaacg ttcttttaaa gaaccctgag cgagattgtt ctgcgtccag
    22381 tcgagaatat tatcgaaagc tgcacggtcg ttagccgcca gggcaaagaa catgccgtaa
    22441 ctttgccctt cggaggtggt gattttgcgc gcgtcgctgg ggtcgatgac gcgcccttcc
    22501 tgactgatgt aatccttttt aaactgctcc caggcaggcc aggtacaggc tgcctgaaca
    22561 ctaaaggcag ccagcagcag catcgtcacg attccactac gcaacacatt catcttcaat
    22621 tactcgttat ccgggttaag acgacgacga ctaataattc gcagcagacg ccacagtacc
    22681 catgccagca atatcacact gatagccgcc agcaccgcca gcagaatcgg atggtttgcc
    22741 agcgcatacc acacgcgctc gaaccacggc agatgaccta cgtaataaac gtcgccaaca
    22801 cgtaggctgt tgataccgga ctcgcggatc accgcgaccg aaccgaacat ggtggcgcgt
    22861 ttgccgctat cgttcaccgc atcgttaagc atttcataac cgcgtgggct atctgccaac
    22921 agcgcaatca cgctgcgctg gtcgttatac ggcgactgga agccaatcac cgccgccatc
    22981 gcaccggaag aggtcagcgt tgaccgggtt tctgccgcgc gatcgctctc gtccggcaca
    23041 atgccgggga acggggtctg gcgcatcggt gttttcaccc agctttcggt cgcctgcacc
    23101 aataggtcga tctgcttatc gtctttcagt ttgtccggga taccaccgat gatcatgatg
    23161 tcggcatctt tgccctgaat ggtgctgcca tcatcggtca ccgtcagatt aatcgccggg
    23221 aagcccgtct gtgcgccgat aaaaccaaca gtattcagca acgtttccat ctgtgcttcg
    23281 ttaggcgctt tcggcatcac ggtgatggtt tgcgacagat ccgccatccg gctgaatggg
    23341 aagcccgcgt tagcaaaggc gcgtagatcc ggcatcggga tgaagtggta atacttcgag
    23401 aagtcgatgg tggagtcgtc accaatcacc acatgattct gcaccggctg gaaggtaata
    23461 cagttatcca ccgaaccgcc cggcatcggg ttcatatact caaagtcgaa gcgcagctgg
    23521 ttggtcgcgc ccagtttcag cgccggaata gagacatctg ttttgccatc cagcaaacct
    23581 tgtaataccg gaatccgcag cagcaggcgg ttcgcctcct gtttgctgct caggttgaag
    23641 gattgcagga actggttatt caggctgata tccatccgcg aactgtcttt caccggcggc
    23701 atggtgtagc ggtaattaat atccatatca atgccggtac tgcgcatcag gtagagatcc
    23761 ggcggcaggt ttagcgaaac gttaatcgct gctggctcaa gaccgctgga ttgtaactgt
    23821 tcttcatagg ttttcagttc gccaaatgtg accggacgat cggtacgtac ccagttcggc
    23881 gcatcgtacg gcttacgcgg tagcagcggt ttcacttcat tcactaccac gctttcacca
    23941 cggaacagaa tgttaccctg agcgatacct ttcgctgcct gcaacaggtc tttgtcgtca
    24001 cgaccaaaca ccaccagcag tttgacgtaa ggattctgcg gatggttaat catctcaatc
    24061 acaggggctt ttaccgccgg atgatcgcgc aggaagtccg gccgtttgtc gttggttgca
    24121 aagacaatgg cattacgatc cggcagttgg ttatagagca ccgggaagtt ctgcccacgc
    24181 cagccagaac gcgaaccaaa ccacgaggcg acaatggcag aggcttgttg cagcccaaca
    24241 tccggcgcac ccgcaaagac catcggcaag gtgttagtac ggttatcgcg cgggtcaaag
    24301 aatggcaccg ggaagtgtga caggtcattc ttcacattca gggtctgata ggtcagatcc
    24361 agtccactgc tccgcccaac atccagccaa agcgtggtgc tggccgggtt ttcgcacacg
    24421 tcctgataat ggccgacaaa ctccagccgc acacggttga agtcggtaat aaacagtggg
    24481 ttaatgggca tttgcgccag cgttttttta cccaactgtt ctttggtcac tggcagcacg
    24541 cccatcagct catcattgag ataaaccttt aactgcgact ggacaggcag taacgatggc
    24601 gatggcgtgt attcgaggtt gagcatcgcc ttcgtcacca cttcatcgct gcgcatacca
    24661 aactcaatgc tgccgttcgg gttaatgcca cgtagcacca tgctgcccgg cggcggcgca
    24721 atttgcgcaa aggtcagctt cacatcacgc gaaggaccgt tctgcgccac gactggcgca
    24781 tcagcgccct gcacgcccgg catcacttgc cccacctgcg gattttgttc cgtctgggcg
    24841 gctacagctg gctcagcatt gatcagtggt tgcgttgctg gcgtcgcctg cgtcatgaaa
    24901 gaggggaacg cactcatccc catagccact gcacaaatcc agaatagttt tcttttcatc
    24961 gcgttatcat cattgttgag ccaaagcctg atccgatggt tgtgccgttt cgctccgctc
    25021 cgggcggcgc ggaataaacg atacaaccca ggaaaccaga gaagtcagca cacggaatat
    25081 gcccttcacc gaagaaggcg caaactccgc cagatggcgg tagccacgga agccgagctt
    25141 cagaatatcc agcagacttt ccagcggctt atcttccggg tagctgtcct gccagagcgc
    25201 ccatgtatcc gcacgggcaa acgtacactg cacaaaatcg atatgttgct gggtggtgag
    25261 cggcattaat ttcagcccaa cttcattacc catcacgcgc gccacctggg tcgggaagac
    25321 gtattcctgc tgaccgcgtt taagcaacag attcactttc tgcccttcca gaatctgcgc
    25381 ctgaccgttg atcttgatcc ccaaaccacc gtcggagaaa tcctgaacgg tacacgagaa
    25441 gaggtgacca tcttcgcggg caattgccgc gggcatcgtc atctccacgc ggtgcgatcg
    25501 gcgtacctgt ttgctttcta ccgataccgc aactgcgccg ccaagaacaa tcaggttgta
    25561 gaacacccac accatactga cgaccacggt gagcatctcg gttggcgggc catagaagta
    25621 gcgccagatg cctaccgcaa cgcccaccag gttgagtaga acaaggaaga tgtagggccg
    25681 cgagatcacc cagtcgacgt actcttcttc caccagtcca cctttggcgg tgacgttaaa
    25741 tttgcctttg tgcgggttaa tcagcgccac cagcgtcggt ggtgcgatat accacgccag
    25801 caccgtttcg tagatttcac tccagaaaga gtggcgatat ttgccctgga tcttcgagtt
    25861 ggtcaggctg gcatggatca tatgcggcag cacgaacagg gcgatcatca acgctggcgc
    25921 atagatgatg taggcatgaa gcagcaggaa cgccagcggc gcagtcagga agatcagtcg
    25981 tggaatgccc gacaagaagt ggaacatggc gttgacgtag cacagccgct gggcgaattt
    26041 cagcccttta ccggtaagcg ggttatcgag tgtaaccagg tgaatcattt gataccgacg
    26101 agggttttag tgaaaggagg taaaatcacc tgtaagatac tgaaataaaa caggtgatgt
    26161 ccggtgaacg cgaaaagtct atttgatacc gattgtgcaa taattagtcg tttatcctaa
    26221 aatcggcaac acttttgata ctacagattg acgcttaaaa ttgctttaaa aaaacttaaa
    26281 acgcttttct ccagtccgta ttgaaaggag tgtgccacag cacaaaattt gcactactcc
    26341 cttacgcacc ggcctttttc tgggttacat ttaaatgatt aaccttagcc tcacattctt
    26401 cataaatctc tcttacagta tcatcgggta actcttgcag actcaatgcc aatgctcttt
    26461 tgaccccact caatgagtgc agcttttgaa tatcagtatc aagtaaaatg gttagtaact
    26521 cggccccttt tctgatcaat aaattggaca aaatctcttg ttcagatgaa gttagcgagt
    26581 cccacgccct catgatgatg gagtcaccat catttacatc ttgttttttt gttctttcta
    26641 tttgttctaa tagttctttt tttgtgattg actcattatt ataaccatag attagccact
    26701 ccattgatac atgttctttt tcagcaatgt tctggagatg gtgctttctt ggctgtggtg
    26761 gtggctgatt gtttctcctt ttgtaatagt tctgcaatgt ctgaatattg attccccagg
    26821 ccctagccgc ctcacttctc gatttgtaac gctctgcaag gcgaaatatt cgctcgatta
    26881 ctggttcttt tccttcgtca agaaaagaac tttttttcat gttcatgcct ttaggttcct
    26941 tcgcgcttat ctaattgatt ttattgatga aaacaatata aagaacaaaa agaacaaaat
    27001 accttgattt ggttcttttc ttgttctatc gtttacccgt acagatatcc gtccgaatat
    27061 ccgtgcgggt aactttttag aatagaggaa gtatgagtag aagagaagtt tcagatcacg
    27121 attggccgcc agaaatcatc aaggcgcgcc tgcacatggc tggcttatca ctgcgctctt
    27181 tatctttaaa ggctggttac agcagggatt cgctgaaaag cgtattacgc accccttgcc
    27241 gcccatatca acaaataatt gctgatgctc ttggtgtgtc acctgaagaa atctggccca
    27301 gcagatacca ggttaagagc tatatgagaa aggcgtcatg atatgtattt cattgcaaaa
    27361 gaactgattg gtgcgcccgg aatgcctgca actacaaaag gtattcgcca ggcattacaa
    27421 cgttacgcac aagggaaaag ctgttgttcc cgtcgtcgct caggatctaa agcaactgaa
    27481 tacagcatcg actgtttacc tgaagtgacg cagcaggcat tacgtgaacg ctacgccctg
    27541 caactgatgg cgcaaaaagc cgatgagtca ccggttccgg tggtgacaaa ggccagacgc
    27601 tcacctgccg tggttgatgc ggtggaggca tatcgcggat caccccaact gatggtcgaa
    27661 cgtatcaatg cactgactga aaaacagcgc caggtggccg atgcacgaat tgcgattgtc
    27721 agcgaggtgc ttaaattcgc gcaacaaccc ggtttcagct gcgctaaagc tatccggttt
    27781 attgttgacc gtctgtcacg ctcgcaactg gacgaacgca ttgtggcaat ggttgaaacg
    27841 gcgaacgcca aaaagggaaa cagccgcgcg ttgagtgaaa tcacgctgaa acgctggatt
    27901 gcggccttta acaaggcgca gaacgccgct gaacgcctac ttttactggc accgggtaaa
    27961 cgtcaggaaa taaaagccga agatattagc tggttgcccg agtttctggc gcagtatcgc
    28021 cagtcaaacg gccgaccaat gaccgaggct tacgaggatt ttgtcgccga atggcagcac
    28081 cggcacgctg atgagcctta tatgctcgat atcatgccct cttatgacac cattcgccgc
    28141 gcgatgaaga aactgccgga agtggtgaaa caaaaaggcc gggtgaccgg cagtgaatac
    28201 cgccagcttg agggattcac gcgccgcgac tggtccagaa tgccggtgaa ttatgtctgg
    28261 attggtgacg gtcacggcat gaagctgaaa tgtgcgcacc cggttcacgg gcgaccattt
    28321 gcaccggaag tgacctttgt tatcgacggt ggcacgcgct ttgtggtggg ctggagcctt
    28381 gacctggctg aaaatgtttt cgccgtagcc ggtgccatac agcacggcat tcgccatcac
    28441 ggcaaaccgt ttctgtatta ctcggataat ggctcggggg aaacggccga catcctggat
    28501 aaggaggttg tggggatact accgcgactg gggattaatc acccgaccgg gattgccggt
    28561 aatccgcagg gacggggcat tatcgaacgg cttaaccgca cattaccgat gcgcatagcc
    28621 cgtaaatacc gcacctattt cgggaaaggt gcagatcgcg agacgttacg taaaaccaac
    28681 cgcgatttac gctcggcatt cagtgccctg caacagggca agcggctgaa cgcccggcag
    28741 cagtcagcga tgcgtgattt accgtcctgg tctgaactga ttgatgccat tcgtgacggc
    28801 gttgagtggt acaacaaccg gccgcacgat gaattaccca taaagccgaa cggtaaacat
    28861 tacagcccgg cggagttcag aaaaaaacgc ctggcggaag aggacacgga aattgaatgg
    28921 ctgtccgatg tggaattgcg ggacatgttc cggccgatgg tggaacgccc tgtaagacgc
    28981 tgtgaaatac gctggctgaa taatatttac tacgcgcccg agctgcgtga tgaacatggc
    29041 cgcaaagtgc ttatcagcta tgacattcat gatgccgaac gaattaccgt acgccgcccg
    29101 gatggcagcg tgatttgcga ggcggtatgg gacggcaata aacgcgaagc cttccctgtc
    29161 agcgcggaat actacaaaca gcagcagcgc cttaaaggta tgcgtaaacg cgcagaggaa
    29221 aaaatccgtg atgccgagga tgaggttgtc aacgtgctgg agcacaagcc gcaggagcca
    29281 tggctggaaa acatataccg ccctgtgggt aatacggtga ccgttcagca accagtcgct
    29341 gacgatgaac ctgacgaaga atacgagcgt aatttccagc ggggtttgca actgctcgaa
    29401 gcgaaattaa aagaaaatga tccgctggcc tgaaataaaa aaaataaccc gaggggtgac
    29461 tcaggttatt tgaggtttct gtctcggaga atgctgtctc aagcgaggtg aataatatga
    29521 ccgatattaa cgatgtaatc aagaccattg atgaacttat tgatggcggc gtactgacgc
    29581 agtatgccat cgccagagag gcgggaattt ccgacggcac attatcggct ttccgtaagg
    29641 ggaaatataa aggcgataac gctgctgtgg ctgcttccct gcgttcctgg tatgagaact
    29701 ggaataaaca aagcgcactg cccgagccgc cgcagtttgt tgaaacgcag acagtccagg
    29761 agctgcgcgc actgtttcag gcggttcgcc tgatgggctg tattaacgtc attgtgggcg
    29821 taccgggtgt gggtaaaacg gccacagccc gtaattactg tcaggagcaa ccgaacacct
    29881 ggatgatcac cctgtcgcct gcgcattcca gcgtcacgga gtgtctgctg gagctggcag
    29941 aggaactggg gattgattac acccgcgcga acaaaggggc attatcccgc gcaatccgcc
    30001 gtcgcctgat gggaacgcgt ggactggtga ttgttgatga ggcggatcat cttggtattg
    30061 acggtctgga gcaactcagg gcaatccagg acgccacggg gatcgggatg gtgcttattg
    30121 gtaacccgcg cggactgttt aaaggtggac gccgcgccgt tgatgattta tcgcgcctgt
    30181 tcagccgtct tgcccgtaca aaacaacttc gcaaggccaa aaaggcggat gtgctggcca
    30241 ttgccagggc atgggggatc agtggtgagg ccgagctggc cgtcatgcag gctatcgctg
    30301 aaaaaccggg agcattacgt gttctgacgc atacgcttaa ccaggcgtgg atcaccgcca
    30361 gtggtgaagg cgctgtgctg acagaaaaac atattaatgc ggcctttaaa gaggtttata
    30421 ccaaccctga attactctca caggtgtgat tatggcggta tttaatattc cggatattta
    30481 cggacgcttt tacctggtga atttcgataa cgtgaaggtg atttcactgg ccgaaaataa
    30541 agaatgtggt gatttacttt ttgaatttaa tgaccgcaca cgaatggtga tatctgccgg
    30601 acttgatcgc gaaggtgcga cagaagttta caggggaata tgccgttctg ttggtgcaaa
    30661 acaagtcaga taaacgaggt gttatatgaa tatgcaatcc tgcggtaaaa aaatgaattt
    30721 attcgactcc ctgaacagtg cacgccgtct gactgaactt gccggtgcgg tactggaacg
    30781 cagcaaacgc tacccacaac gttatgccct gaaaaccacg ccgccggtaa gcgaagttaa
    30841 gggaaccggt gaaattgaaa tcaccataca gaccaacggc ctgcgccgcc gtgtgaaggc
    30901 tacccgcatc agcggctgca cggtttactg ggaggagtga ggtgaaacga aatctaattg
    30961 cgtgggcgtg gtcgagcggt ctcattgaac tgggttacgt cctgccggaa ggtgcattgc
    31021 cgatagttgc cggaaagcct gccacagtac ggcatgtgat tgaggttatg gcgcgtcatg
    31081 gacgtgatga acaggagcag ttactggttc cggggatacc ggaagcggtg acggaggaag
    31141 aagcctttaa tgccatgatt cggttctgcc gtgaggtcag acgccgggtc agttatccaa
    31201 acagaacgag gaccagagga tgagtaaagt cgtacgcatt attttcgaat acaaggagca
    31261 cgttatccat aaaaacgcgg atggaacagt gcgcatgggg ataagtctgg acatacgttc
    31321 aaccgggata aagcagaaag gtgatggacc tgccatgatt tttggggtgg ttatgctcgc
    31381 ggaaagcaga gactttgctg aacttgtggc aatgaaagcc agtgcgctca tgaaagatat
    31441 gagcatgcgt tccggggtta ttaaaggtaa tgaatttaat ca
//
LOCUS       DAZFQD010000050        31206 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.050, whole genome shotgun sequence.
ACCESSION   DAZFQD010000050 DAZFQD010000000
VERSION     DAZFQD010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31206
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.050"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(123..386)
                     /gene="cedA"
                     /locus_tag="ACHKTW_002306"
     CDS             complement(123..386)
                     /gene="cedA"
                     /locus_tag="ACHKTW_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HFO6463318.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
     gene            569..2830
                     /gene="katE"
                     /locus_tag="ACHKTW_002307"
     CDS             569..2830
                     /gene="katE"
                     /locus_tag="ACHKTW_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HFO6463319.1"
                     /translation="MSQHNEKNPHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGNRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKALK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSADGSFMDELLTLMAAHR
                     VWSRIPKIDKIPA"
     gene            complement(2877..3635)
                     /gene="chbG"
                     /locus_tag="ACHKTW_002308"
     CDS             complement(2877..3635)
                     /gene="chbG"
                     /locus_tag="ACHKTW_002308"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HFO6463320.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMHFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVVRFAAEEGIALRIDRQPLSNAG
                     DLPANLRSSQGFSSAFYGEEISEALFLQVLDDSSHRRERSLEVMCHPAFVDNLIRKSA
                     YCFPRLTELEVLTSASLKYAIAERGYRLGSYLDV"
     gene            complement(3648..5000)
                     /gene="celF"
                     /locus_tag="ACHKTW_002309"
                     /gene_synonym="chbF"
     CDS             complement(3648..5000)
                     /gene="celF"
                     /locus_tag="ACHKTW_002309"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFO6463321.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEDG
                     KEKLDIIFELCQRMIDNAGVPMKLYKTLDRREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIRDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRF
                     AELLDGVASGQLKASSVKNIFDLPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCKLGRDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            complement(5105..5947)
                     /gene="chbR"
                     /locus_tag="ACHKTW_002310"
     CDS             complement(5105..5947)
                     /gene="chbR"
                     /locus_tag="ACHKTW_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
                     /protein_id="HFO6463322.1"
                     /translation="MMQPVINAPEIATAREQQLFNGKNFHVFIYNKTESISGLHQHDY
                     YEFTLVLTGRYFQEINGKRVLLERGDFVFIPLGSHHQSFYEFGATRILNVGISKRFFE
                     QHYLPLLPYCFVASQVYRTNNAFLTYVETVISSLNFRETGLEEFVEMVTFYVINRLRH
                     YREEQVIDDVPQWLKSTVEKMHDKEQFSESALENMVTLSAKSQEYLTRATQRYYGKTP
                     MQIINEIRINFAKKQLEMTNYSVTDIAFEAGYSSPSLFIKTFKKLTSFTPKSYRKKLT
                     EFNQ"
     gene            complement(5955..6305)
                     /gene="chbA"
                     /locus_tag="ACHKTW_002311"
     CDS             complement(5955..6305)
                     /gene="chbA"
                     /locus_tag="ACHKTW_002311"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HFO6463323.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            complement(6356..7714)
                     /gene="chbC"
                     /locus_tag="ACHKTW_002312"
     CDS             complement(6356..7714)
                     /gene="chbC"
                     /locus_tag="ACHKTW_002312"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
                     /protein_id="HFO6463324.1"
                     /translation="MSNVIASLEKVLLPFAVKIGKQPHVNAIKNGFIRLMPLTLAGAM
                     FVLINNVFLSFGEGSFFYSLGIRLDASTIETLNGLKGIGGNVYNGTLGIMSLMAPFFI
                     GMALAEERKVDALAAGLLSVAAFMTVTPYSVGEAYAVGANWLGGANIISGIIIGLVVA
                     EMFTFIVRRNWVIKLPDSVPASVSRSFSALIPGFIILSVMGIIAWALNTWGTNFHQII
                     MDTISTPLASLGSVVGWAYVIFVPLLWFFGIHGALALTALDNGIMTPWALENIATYQQ
                     YGSVEAALAAGKTFHIWAKPMLDSFIFLGGSGATLGLILAIFIASRRADYRQVAKLAL
                     PSGIFQINEPILFGLPIIMNPVMFIPFVLVQPILAAITLAAYYMGIIPPVTNIAPWTM
                     PTGLGAFFNTNGSVAALLVALFNLGIATLIYLPFVVVANKAQNAIDKEESEEDIANAL
                     KF"
     gene            complement(7799..8119)
                     /gene="chbB"
                     /locus_tag="ACHKTW_002313"
     CDS             complement(7799..8119)
                     /gene="chbB"
                     /locus_tag="ACHKTW_002313"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HFO6463325.1"
                     /translation="MEKKHIYLFCSAGMSTSLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAAN"
     gene            complement(8418..8756)
                     /gene="osmE"
                     /locus_tag="ACHKTW_002314"
     CDS             complement(8418..8756)
                     /gene="osmE"
                     /locus_tag="ACHKTW_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HFO6463326.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            8958..9785
                     /gene="nadE"
                     /locus_tag="ACHKTW_002315"
     CDS             8958..9785
                     /gene="nadE"
                     /locus_tag="ACHKTW_002315"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HFO6463327.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSVDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRQETGNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLAALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNVPEQVARTIENWYLKTEHKRRPPITVFDDFWKK"
     gene            10015..10902
                     /gene="cho"
                     /locus_tag="ACHKTW_002316"
     CDS             10015..10902
                     /gene="cho"
                     /locus_tag="ACHKTW_002316"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HFO6463328.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLRSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQSIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEEATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(10862..11437)
                     /gene="ves"
                     /locus_tag="ACHKTW_002317"
     CDS             complement(10862..11437)
                     /gene="ves"
                     /locus_tag="ACHKTW_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HFO6463329.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMFLESADRFNHTLKPLQPFAFAADQVVKAKLTAGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(11640..12125)
                     /gene="spy"
                     /locus_tag="ACHKTW_002318"
     CDS             complement(11640..12125)
                     /gene="spy"
                     /locus_tag="ACHKTW_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone Spy"
                     /protein_id="HFO6463330.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIREIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
                     E"
     gene            complement(12455..13423)
                     /gene="astE"
                     /locus_tag="ACHKTW_002319"
     CDS             complement(12455..13423)
                     /gene="astE"
                     /locus_tag="ACHKTW_002319"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0009017 - succinylglutamate desuccinylase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HFO6463331.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDLGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSARHFGALACTLELGKALPFG
                     QNDHRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            complement(13416..14759)
                     /gene="astB"
                     /locus_tag="ACHKTW_002320"
     CDS             complement(13416..14759)
                     /gene="astB"
                     /locus_tag="ACHKTW_002320"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009015 - N-succinylarginine dihydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HFO6463332.1"
                     /translation="MNAWEVNFDGLVGLTHHYAGLSFGNEASTRHRFQVSNPRQAAKQ
                     GLLKMKALADAGFPQAVIPPHERPFIPVLRQLGFSGSDEQVLEKVARQAPHWLSSVSS
                     ASPMWVANAATIAPSADTLDGKVHLTVANLNNKFHRSLEAHVTESLLKAIFNDEEKFS
                     VHSALPQVALLGDEGAANHNRLGGHYGEPGMQLFVYGREEGNDTRPSRYPARQTREAS
                     EAVARLNQVNPQQVIFAQQNPDVIDQGVFHNDVIAVSNRQVLFCHQQAFARQSQLLAN
                     LRARVNGFMAIEVPATQVSVSDAVSTYLFNSQLLSRDDGSMMLVLPQECREHAGVWGY
                     LNELLAADNPISELKVFDLRESMANGGGPACLRLRVVLTEEERRAVNPAVMMNDTLFN
                     ALNDWVDRYYRDRLTAADLADPQLLREGREALDVLSQLLNLGSVYPFQREGGGNG"
     gene            complement(14756..16234)
                     /gene="astD"
                     /locus_tag="ACHKTW_002321"
     CDS             complement(14756..16234)
                     /gene="astD"
                     /locus_tag="ACHKTW_002321"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043824 - succinylglutamate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HFO6463333.1"
                     /translation="MTLWINGDWITGQGASRVKRNPVSGEVLWQGNDADAAQVEQACR
                     AARAAFPRWARLSLAERQVVVERFAGLLERNKGELTAIIARETGKPRWEAATEVTAMI
                     NKIAISIKAYHARTGEQRSEMPDGAASLRHRPHGVLAVFGPYNFPGHLPNGHIVPALL
                     AGNTIIFKPSELTPWSGEAVMRLWQQAGLPPGVLNLVQGGRETGQALSALEDLDGLLF
                     TGSANTGYQLHRQLSGQPEKILALEMGGNNPLIIDEVADIDAAVHLTIQSAFVTAGQR
                     CTCARRLLLKSGAQGDAFLARLVAVSQRLTPGNWDDEPQPFIGGLISEQAAQQVVTAW
                     QQLEAMGGRTLLAPRLLQSETSLLTPGIIEMTGVAGVPDEEVFGPLLRVWRYDSFEEA
                     ILMANNTRFGLSCGLVSPEREKFDQLLLEARAGIVNWNKPLTGAASTAPFGGIGASGN
                     HRPSAWYAADYCAWPMASLESDSLTLPATLNPGLDFSDEVVR"
     gene            complement(16231..17265)
                     /gene="astA"
                     /locus_tag="ACHKTW_002322"
     CDS             complement(16231..17265)
                     /gene="astA"
                     /locus_tag="ACHKTW_002322"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008791 - arginine N-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HFO6463334.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHALKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLARTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            complement(17262..18482)
                     /gene="astC"
                     /locus_tag="ACHKTW_002323"
     CDS             complement(17262..18482)
                     /gene="astC"
                     /locus_tag="ACHKTW_002323"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
                     /protein_id="HFO6463335.1"
                     /translation="MSQPITRENFDEWMIPVYAPAPFIPVRGEGSRLWDQQGKEYIDF
                     AGGIAVNALGHAHPELREALNEQASKFWHTGNGYTNEPVLRLAKKLIDATFADRVFFC
                     NSGAEANEAALKLARKFAHDRYGSHKSGIVAFKNAFHGRTLFTVSAGGQPAYSQDFAP
                     LPADIRHAAYNDINSASALIDDSTCAVIVEPIQGEGGVVPASNAFLQGLRELCNRHNA
                     LLIFDEVQTGVGRTGELYAYMHYGVTPDLLTTAKALGGGFPVGALLATEECARVMTVG
                     THGTTYGGNPLASAVAGKVLELINTPEMLNGVKQRHDWFVERLNTINHRYGLFSEVRG
                     LGLLIGCVLNADYAGQAKQISQEAAKAGVMVLIAGGNVVRFAPALNVSEEEVTTGLDR
                     FAAACEHFVSRGSS"
     gene            18928..19734
                     /gene="xthA"
                     /locus_tag="ACHKTW_002324"
     CDS             18928..19734
                     /gene="xthA"
                     /locus_tag="ACHKTW_002324"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFO6463336.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSLLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRDNPVLIMGDMNISPTDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMDRLMSWGLVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            19901..20611
                     /locus_tag="ACHKTW_002325"
     CDS             19901..20611
                     /locus_tag="ACHKTW_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HFO6463337.1"
                     /translation="MNAERNFLFACLIFALVIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     LFGYSLYILLFIIATLFLLPGSILVIAGGIVFGPLLGTLLSLIAATLASSCSFLLARW
                     LGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGLTTIAFWP
                     YTLISALTTLPGIVIYTVMASDLANEGITLRFILQLCLAGLALFILVQLAKLYARHKH
                     VDLSASRRSPLTHPKNEG"
     gene            20616..21293
                     /gene="ydjY"
                     /locus_tag="ACHKTW_002326"
     CDS             20616..21293
                     /gene="ydjY"
                     /locus_tag="ACHKTW_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YdjY"
                     /protein_id="HFO6463338.1"
                     /translation="MSQHYSVSWKKGLAALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMAYATPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKRGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            21308..22015
                     /locus_tag="ACHKTW_002327"
     CDS             21308..22015
                     /locus_tag="ACHKTW_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HFO6463339.1"
                     /translation="MMKIQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAAGLTSIRFRSFFIATGLGQLPATIVYSWAGSMLTGGTFWFVTGLFILFALTV
                     VIFMAKKIWLERQKRNA"
     gene            22015..22563
                     /locus_tag="ACHKTW_002328"
     CDS             22015..22563
                     /locus_tag="ACHKTW_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFO6463340.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSTDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASVTPPTVDDALRTRLAAHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            22573..23739
                     /locus_tag="ACHKTW_002329"
     CDS             22573..23739
                     /locus_tag="ACHKTW_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFO6463341.1"
                     /translation="MRHCGWLLGLLSLFSLATHASDWQEIKNEAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYAINLKIVRLADAADAVKRIQTEAAAGRKTGGSVDLLWVNGE
                     NFRTLKEANLLQTGWAETLPNWRYVDTQLPVREDFSVPTEGAESPWGGAQLTFIARRD
                     VTPQPPQTPQALLEFAKANPGTVTYPRPPDFTGTAFLEQLLIMLTPDPAALKVAPDKA
                     TFARVTAPLWQYLDALHPYLWRKGKDFPPSPARMDALLKAGTLRLSLTFNPAHAQQKI
                     ASGDLPASSYSFGFNQGMIGNVHFVTIPANANASAAAKVVANFLLSPNAQLRKADPAV
                     WGDPSVLDPQKLPDGQRETLQSRMPQDLPPVLAEPHAGWVNALEQEWLHRYGTH"
     gene            23712..25247
                     /locus_tag="ACHKTW_002330"
     CDS             23712..25247
                     /locus_tag="ACHKTW_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFO6463342.1"
                     /translation="MATPLRYALIFLLWAMVAVIYAPLIPAALTLISPALSLTHWQAL
                     FADPQLPQALLATLVSTTIAAVGALLIALLVIVALWPWPKWQRMCARLPWLLAIPHVA
                     FATSALLLFAEGGLLYDYFPYFTPLMDKLGIGLGLTLAVKESAFLLWILAAVLSEKRL
                     LQQVIVLDSLGYSRWQCLNWLLLPSVAPALAMAMLAIVAWSLSVVDVAIILGPGNPPT
                     LAVISWQWLTQGDADQQTKGALASLLLMLLLAAYVLLSYLLWRSWRRTIPRVDGIRKP
                     ATPLLPGTTLASFLPLTGVLCVVLLAILADQSTINSEALINSLTMGLVATFIALLLLL
                     LWLEWGPQRRQLWLWLPILLPALPLVAGQYTLALWLNLDGSWTAVVWGHLLWVMPWML
                     FILQPAWQRIDSRLILIAQTLSWSRAKIFFYVKCPLMLRPTLIAFAVGFSVSIAQYMP
                     TLWLGAGRFPTLTTEAVALSSGGSNGIFAAQALWQLLLPLIIFALTALVAKWVGYVRQ
                     GLR"
     gene            25247..25900
                     /locus_tag="ACHKTW_002331"
     CDS             25247..25900
                     /locus_tag="ACHKTW_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFO6463343.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     STLKGTARRNAVKDALDRAGLAETYHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRELAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            25967..27274
                     /gene="ynjE"
                     /locus_tag="ACHKTW_002332"
     CDS             25967..27274
                     /gene="ynjE"
                     /locus_tag="ACHKTW_002332"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
                     /protein_id="HFO6463344.1"
                     /translation="MKRVSQMTALAMALGLACASSWAAELAKPLTPDQLQQQNGKAID
                     TRPSAFYNGWPQTLNGPSGHEPAALNLSASWLDKMSTEQLNAWIKQHNLKADAPVALY
                     GNDKDVDAVKTRLQKAGFTHISILSDALSEPSRLQKLPHFEQLVYPQWLHDLQQGKEV
                     TAKPAGDWKVIEAAWGAPKLYLISHIPGADYIDTNEVESEPLWNKVSDEQLKAMLAKH
                     GIRHDTTVILYGRDVYAGARVAQIMLYAGVKDVRLLDGGWQTWSDAGLPVERGTPPKV
                     KAEPDFGVKIPAQPQLMLDMEQARGLLHRQDASLVSIRSWPEFIGTTSGYSYIKPKGE
                     IAGARWGHAGSDSTHMEDFHNPDGTMRSADDITAMWKAWNIKPEQQVSFYCGTGWRAS
                     ETFMYARAMGWKNVSVYDGGWYEWSSDPKNPVATGERGPDSSK"
     gene            complement(27283..27903)
                     /locus_tag="ACHKTW_002333"
     CDS             complement(27283..27903)
                     /locus_tag="ACHKTW_002333"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFO6463345.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRGLTDAGGFLDISLDFLFYALVPFGFIL
                     AAPQQNALAGGWLLFAFIGTGSSFLAFAALAAKHQIDNPGYAHKSFYYLGGLTEGTET
                     ILLFVLGCLFPAWFAWFAWIFGALCWMTTFTRVWSGYLTLKSHQRQ"
     gene            27990..28397
                     /gene="nudG"
                     /locus_tag="ACHKTW_002334"
     CDS             27990..28397
                     /gene="nudG"
                     /locus_tag="ACHKTW_002334"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HFO6463346.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKVEPDES
                     QRQALVRELREELGIEATVGEYVTSHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARPAD"
     gene            complement(28363..28635)
                     /locus_tag="ACHKTW_002335"
     CDS             complement(28363..28635)
                     /locus_tag="ACHKTW_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HFO6463347.1"
                     /translation="MSRALFAVVLAFPLIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDKNYSEGAVIKAEGILLQCQRDDKTLSTNPLVWRRVKP"
     gene            28871..30214
                     /gene="gdhA"
                     /locus_tag="ACHKTW_002336"
     CDS             28871..30214
                     /gene="gdhA"
                     /locus_tag="ACHKTW_002336"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFO6463348.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQMSLLERLVEPERVIQFRVVWVDDRNQVQVNRAWRVQFSSAIGPYKGGMRFHPSVN
                     LSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGEVMRFCQALMTELYRHLGA
                     DTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYF
                     TEAMLKRHGMGFEGMRVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTK
                     EKLARLIEIKASRDGRVADYAKEFGLVYLEGQQPWSVPVDIALPCATQNELDVDAAHQ
                     LIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLG
                     WKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLAQGVI"
     gene            complement(30331..>31206)
                     /locus_tag="ACHKTW_002337"
     CDS             complement(30331..>31206)
                     /locus_tag="ACHKTW_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFO6463349.1"
                     /translation="FAAITSNGLNERGLITAGCSFLYLLIMMGLIVRAGFKAKKEQLH
                     YYQAKGIEPLSIEKLQALQLIAPYRFYHKQWSETLEFWPRKPEPGKDTFQYHVLPFDS
                     IDIISKRRESLEDQWGIEDSESYCALMEHFLSGDHGANTFKANMEEAPEQVIALLNKF
                     AVFPSDYISDCANNRSGKSSAKLIWAAELSWMISISSTAFQNGTIEEELAWHYIMLAS
                     RKAHELFESEEDYQKNSLMGFLYWHICCYRRKLTDAELEACYRYDKQFWEHYSKKCRW
                     PIRNVPWGASSVKYS"
BASE COUNT         7687 a         8234 c         7703 g         7582 t
ORIGIN      
        1 ttatttatag caatagctcg tcaaaggcag ctttttgtta caggtggttt gaatgaatgt
       61 agcaacgaaa tgcagaattt caggtcatgt aactcccggc aaaaccggga ggtatgtaat
      121 ccttactcag tcacttcccc ttcctggcgg atctgatttg cccaacgttg ggcagattca
      181 ggcacagtaa acgccggtga gcgcagaaat gactctccca tcagtacaaa cgcaacatat
      241 ttgccacgca gcatccagac atcacgaaac gaatccatct ttatcgcatg ttctggcggc
      301 gcgggttccg tgcgtgggac atagctaata atctggcggt tttgctggcg gagcggtttc
      361 ttcattactg gcttcactaa acgcatatta aaaattagaa aaactgtagt ttagccgatt
      421 tagcccctgt acgtcccgct ttgcgtgtat ttcataacac cgtttccaga atagtctccg
      481 aagcgggatc tggctggtgg tctatagtta gagagttttt tgaccaaaac agcggccctt
      541 tcagtaataa attaaggaga cgagttcaat gtcgcaacat aacgaaaaga acccacatca
      601 gcaccagtca ccactacacg attccagcga agcgaaaccg gggatggact cactggcacc
      661 tgaggacgga tctcatcgtc cagcggctga accaacaccg ccaggtgcac aacctaccgc
      721 ccctgggagc ctgaaagccc ctgatacacg taacgaaaaa cttaattctc tggaagacgt
      781 acgcaaaggc agtgaaaatt atgcgctgac cactaatcag ggcgtgcgca tcgccgatga
      841 tcaaaactca ctgcgtgccg gtaaccgtgg tccaacgctg ctggaagatt ttattctgcg
      901 cgagaaaatc acccactttg accatgagcg catcccggaa cgtattgttc atgcacgcgg
      961 atcagcagct cacggttatt tccagccata taaaagctta agcgatatca ccaaagcgga
     1021 tttcctctca gatccgaaca aaatcacccc agtatttgtt aggttctcta ccgttcaggg
     1081 tggtgctggc tctgccgata ccgtgcgtga tatccgtggc tttgccacca aattctatac
     1141 cgaagagggt atttttgacc ttgttggcaa taacacgcca atcttcttta tccaggatgc
     1201 gcataaattc cccgattttg ttcatgcggt aaaaccagaa ccacactggg caattccaca
     1261 agggcaaagc gcccacgata ctttctggga ttatgtttct ctgcaacctg aaactctgca
     1321 caacgtgatg tgggcgatgt cggatcgcgg catcccccgc agttaccgca ccatggaagg
     1381 cttcggtatt cacaccttcc gcctgattaa tgccgaaggg aaggcaacgt ttgtacgttt
     1441 ccactggaaa ccactggcag gtaaagcctc actcgtttgg gatgaagcac aaaaactcac
     1501 cggacgtgac ccggacttcc accgccgcga gttgtgggaa gccattgaag caggcgattt
     1561 tccggaatac gaactgggct tccagttgat tcctgaagaa gatgaattca agttcgactt
     1621 cgatcttctc gatccaacca aactgatccc ggaagaactg gtgcccgttc agcgtgtcgg
     1681 caaaatggtg ctcaatcgca atccggataa cttctttgcc gaaaacgaac aagcagcatt
     1741 ccatccaggt catattgttc ctggtctgga cttcaccaac gatccgctgt tgcagggacg
     1801 tttgttctcc tataccgata cacaaatcag tcgtcttggt gggccgaatt tccatgagat
     1861 tccgattaac cgtccgacct gcccttacca caacttccag cgtgatggca tgcatcgtat
     1921 ggggatcgac actaacccgg cgaattacga accgaactcg atcaacgata actggccgcg
     1981 cgaaacaccg ccgggaccga aacgcggcgg ttttgaatca tatcaggaac gcgtggaagg
     2041 aaataaagtt cgtgagcgca gtccatcgtt tggcgaatat tattcccatc cgcgtctgtt
     2101 ctggctaagt cagacgccat tcgagcagcg ccatattgtc gatggtttca gttttgagtt
     2161 aagcaaagtc gttcgtccgt atattcgtga gcgcgttgtt gaccagctgg cgcatattga
     2221 tctcactctg gcccaggcgg tggcgaaaaa tctcggtatc gaactgactg acgaccagct
     2281 gaatatcacc ccacctccgg acgtcaacgg tctgaaaaag gatccatcct taagtttgta
     2341 cgccattcct gacggtgatg tgaaaggtcg cgtggtagcg attttactta atgatgaagt
     2401 gagatcggca gaccttctgg ccattctcaa ggccctgaag gccaaaggcg ttcatgccaa
     2461 actgctctac tcccgaatgg gtgaagtgac tgcggatgac ggtacggtgt tgcctatagc
     2521 cgctaccttt gccggtgcac cttcgctgac ggtcgatgcg gtcattgtcc cttgcggcaa
     2581 tatcgcggat atcgctgaca acggcgatgc caactactac ctgatggaag cctacaaaca
     2641 ccttaaaccg attgcgctgg cgggagacgc gcgcaagttt aaagcaacaa tcaaggtcgc
     2701 tgaccagggt gaagaaggga ttgtggaagc tgacagcgct gacggtagtt ttatggatga
     2761 actgctaacg ctgatggcag cacaccgcgt gtggtcacgc attcctaaga ttgacaaaat
     2821 tccggcgtaa atatggataa ggtcacgtcc cctggggagt gaccttacca ccttacttac
     2881 acatcaagat aactacccaa ccgataacca cgctcagcaa ttgcatattt caacgacgct
     2941 gatgtcagca cttccagctc cgttaaccgt gggaaacagt aagcactctt gcgaatcaaa
     3001 ttatcgacaa acgccggatg gcacatgact tccagcgagc gttccctgcg atggctggaa
     3061 tcatccagca cttgcaggaa cagtgcctcg ctaatctctt caccataaaa cgcgctactg
     3121 aatccctgcg aactgcgcag attagccggt aaatcaccag cgttagatag cggctgacgg
     3181 tcgatgcgca acgcaatccc ctcttcagcc gcaaacctga caacaatcgg gaaaatctgc
     3241 gggaacatat gcacatgatg atggctatca agatgcgtag gtttgcgtcc aaatagctca
     3301 atgaaacgca aatactgact ggcaagctcc tgagtaattt cttccagcgg taaagcatct
     3361 tcttctgcca actgccagat ccattttccc agcacaccat cgcgggttaa ccccggcata
     3421 gctgtcagtg gcttgcccat agtaaggaca aagtgcatcc ctatggccag actcggttca
     3481 tcacgactca actgcaccgc atggtcaata gcctgcccat tcaccagcgc cgtcgtcgat
     3541 gtgacaatcc cattgcgaca ggcctcgata atgccgtagt tctgaccttt gcttaagcca
     3601 aaatcatcgg cattaacaat cagtaagcgt tccataatca gcctcggtta atgtgctttt
     3661 ttaagctctg cgatgcagtc ggcaaaattt ggcagccatt tctcgtgcgc cagaatcatc
     3721 tcgcgtgcca gcagctcagc atcgcgatcg gaatgcacca acggactaag gtttagcgcc
     3781 agtaacacat cgttaaattc tccgctaagt gcggcgttgc tggcagcaat ctcgaagcct
     3841 ttaatggtgt gaatcagccc catcacttta tcatcgaaat gcgtaatgcg cggatgtggc
     3901 gtcgcgccat cgcgccccag cttacaggtc atttctaccg cccaatctgc cggaatatta
     3961 tcaatatgcc catgatgcgg gatattaacg taatgttcag cttgcttgtc gttgtagata
     4021 gcgttgatca cttcgcacgc tgcatcagag taataagccc caccgcgctg ttccagttct
     4081 ttcggcttaa ctttcaactc cggattttta tacagctcaa aaagttgttt ctcgactttc
     4141 tgtactacct gtgctcgtgc gccgcctttg tagtattcgc ccatttcaat agccagcatc
     4201 tctttctgct tgaagtaata cagcagataa gaacatggca gcagatttaa cgaacgaatt
     4261 aagccctcac taaatggcag atcgaaaata ttttttacag aggacgcttt taactgccct
     4321 gacgccacac catcaagcaa ttcggcaaag cgcgacttgc catttaccag cacatcctta
     4381 atgaacacca tatggttgag gccgaacaga tcgatagata aatcatcgct gtctttcagc
     4441 atcagaacat cgcgaataaa catcttcatg ccgatcggaa tattacacac gccgataaag
     4501 cgtttaaatc cggtatgacg ataaacggct tcagtgacca ttcccgccgg gttagtgaag
     4561 ttaatcaccc atgcattcgg acaaagttct tcgacatctt ttacgatgtc aaaaatcacc
     4621 ggaatggtac gcagaccttt aaacagaccg cccgcgccgt tggtttcctg accaagataa
     4681 ccatgactta atggaatacg ttcatccagt tcgcgcgccg gtaattggcc tacgcgcagt
     4741 tgggtagtaa cgaaatcagc atctttcaat gcttcgcggc gatccagcgt tttataaagc
     4801 ttcatcggga cgccagcgtt atcaatcatc cgttggcaga gttcaaaaat gatatccagt
     4861 ttctctttac catcttcgac atccaccagc cataattcgc tgaccggcaa ttcgtgataa
     4921 cgcttaataa atccttccag taactccggg gtatagctgc tcccgccacc aatagtgacg
     4981 acttttaatt tctggctcat aatttctccc ttcagtacag aatactgata tctggcatat
     5041 ctgccccccc ggacataaat aatccagcaa cagtacagat atgtgaattg tcaggtataa
     5101 cgacttactg attaaattca gtcaatttct tacgatagct cttaggcgta aaggacgtta
     5161 atttcttaaa cgttttaata aacaaactcg ggctactata accggcctca aacgcaatat
     5221 ccgtcacgga atagttggtc atttccagtt gttttttggc aaaattaata cggatctcat
     5281 taataatctg cattggtgtt ttgccataat atcgttgagt cgctcgcgtc aaatattcct
     5341 gtgatttggc tgacaacgtc accatattct ccagcgccga ttcactaaac tgctctttat
     5401 catgcatctt ttctaccgta cttttcagcc actgcggtac atcatcaatc acctgttctt
     5461 cgcggtaatg acgtaaacgg ttaatgacat aaaaagtaac catctcaaca aactcttcca
     5521 gccctgtttc gcggaaattc aatgaagaaa tcactgtttc cacataggtg agaaaagcgt
     5581 tattggtccg gtatacctgc gaagcgacaa agcaataagg caacaacggc aggtaatgct
     5641 gctcaaaaaa gcgtttactg atcccaacgt tcaatatacg cgtggcacca aactcataaa
     5701 aactttgatg gtgcgaacct aacggaataa aaacaaaatc gccccgttcc agtaacacgc
     5761 gcttaccgtt aatctcctgg aaataacgcc cggttaatac cagagtaaat tcataatagt
     5821 cgtgctgatg cagcccgctg atactctcag ttttgttata gataaacaca tggaagtttt
     5881 tgccattaaa caactgctgt tctcgggcag tggcaatttc cggcgcgtta atcactggct
     5941 gcatcatcga ctccttatgc cttcagtttt tcatgaagct caattaattc agtaatcagt
     6001 tcacgcgcaa gcatggacgt cattaaatga tcctgagcgt ggaccagcac cagactcact
     6061 ttcatcttac cttcgcccgc atcgccttca atcagtttcg tctgtaccag atgcgcttca
     6121 ttcaatgcca tccgtgactg atccatcatg gcttttgctg cagcaaaatc gccctgcttc
     6181 gcctgtttca gtgccgcata cgccaggctg cgcgcttgtc cggagttgat gataagcccc
     6241 atcaccactt cttccagctc ttcagcttcc gtttgcgtat cgggaatgtt atcgagatcc
     6301 atcatacatc gttcctctct attcttaccg gcacgattac ccgtaccggc atcgattaaa
     6361 atttcagggc attagcgata tcttcttcgc tctcttcttt atcaatcgcg ttttgtgctt
     6421 tgttagccac cacaacaaag ggcagataaa ttaacgttgc gataccaagg ttgaagagtg
     6481 cgaccagcaa tgcggcgacg ctaccgttgg tgttaaagaa ggctcccaga ccggttggca
     6541 tggtccacgg tgcaatattg gtcaccggag gaatgatgcc catgtagtac gctgcgaggg
     6601 tgattgccgc cagaatcggt tgtaccagta caaacgggat aaacatcacc gggttcatga
     6661 taattggcag accaaacaga atcggttcgt taatctggaa gatgccggac ggcagcgcca
     6721 gttttgccac ctgacgataa tcagcacggc gagaggcgat aaagatagcc aggatcaggc
     6781 ctaaagtcgc accactgccc ccaaggaaaa taaaggagtc cagcatcggc ttggcccaga
     6841 tatggaaggt cttaccggct gccagcgccg cttcgacgga accatattgc tgataggtcg
     6901 cgatattttc cagcgcccac ggcgtcataa tgccgttgtc cagtgcggtc agcgccagcg
     6961 cgccatgaat accgaagaac cagagcagtg gaacaaagat cacataggcc cagcccacca
     7021 cgctacccaa cgatgccagt ggggttgaga tggtatccat aatgatctga tggaagttgg
     7081 tgccccaggt attcaacgcc caggcaataa tccccatcac ggaaagaata ataaagccgg
     7141 gaattaatgc cgagaaggaa cgcgatactg aagcaggtac gctgtcgggc agtttaatga
     7201 cccaattgcg gcggacaata aaggtaaaca tttctgccac caccaggcca ataataatcc
     7261 cggagatgat attcgcccca cctaaccagt ttgcgccaac cgcataggcc tcaccgacac
     7321 tatatggggt gacggtcata aatgctgcaa cggataacaa cccagccgcc agcgcatcga
     7381 ctttacgctc ttctgccagc gccatgccaa taaagaacgg tgccattaaa gacattatcc
     7441 ctaatgttcc gttatatacg ttgccgccaa tacctttcag accattaagt gtttcaatgg
     7501 ttgaggcgtc gaggcgaata cctaaggaat aaaaaaacga cccctcccca aagcttagaa
     7561 aaacgttgtt aattaataca aacatggccc ccgcaagggt taacggcatt aagcgaataa
     7621 agccattttt gattgcatta acgtgtggct gctttcctat tttaactgca aaagggagga
     7681 gtaccttttc aagcgatgca ataacattac tcatagaaaa atacccttaa aaaccgcaat
     7741 ttaaatattg cggtattgat ttatgaaata actctttgac gggaaaattt aaaataaatt
     7801 aatttgctgc ggctttttta atcgctgcaa ccgcagcctt aagcacgcct aaaccatcga
     7861 ctttgccata aagcagcgag tcaattactt caaccggttt gttgggtaac aaacgctgga
     7921 tttcgggcaa catataagca atctgcggcc ctaataacac gacatcggca ttctgacctt
     7981 tttcaccagc cagtgtttcc ggaaatgctt caataatgac cggaacttca tatttttctg
     8041 cctgtgcgcg catttttgat accagtaaag aggtagacat gcccgcagaa caaaacagat
     8101 aaatgtgttt cttttccata aaactgccct catcgacgat tatctgtcag ccagacactc
     8161 cgcaagcctt aacctgcttc catgctctgg gtaacttgcg aaaccaacat gacttttttt
     8221 gtggctgaaa ggagtatacg gtatagacct gagcaatagt atttctttga ccttataaat
     8281 tgtttctcct tgacctgtgg ttatgaccct cttcacattt cgggcaaata attcgcgaag
     8341 ataattaaga actcaggccc aaaaaaaccg gcgcaatggc cggtttccgt tgttactcaa
     8401 gctttcagac gaattgatta cttcgcagcc tgtggatcag tgtcgtattc agcacaggtc
     8461 tgataaccgg agttgatgac atgtccggta tcatctaacg cgacaaagta ggtttctgct
     8521 ttaccatcac gttgacccag gatgtaggtc tggcaagtac cgcgagcatg gatcatgctc
     8581 acttcagacg aaggtttacc cgcaatttgt gcaacctgcg cccggctcat gccttttttg
     8641 acgtctttca ccacaggctg tacaaactgg tctttagtac gatcataagc cgtacaaccc
     8701 gccagcatgg ttaataccgc cgctgcactc agaattcctg ccatattctt gttcatattc
     8761 cgtcctcttg tttatcagcg tgttagataa gcctggaata cattgggcgc tttttcaagc
     8821 ccgtgaacga aacagctccg ctttcagagg attcctgtat gacgttttaa ccaccattca
     8881 gcccgctgtc gcttgtcgtt tcagtagcaa cgggttagct ttaaggaagt tttgtctttt
     8941 ctgtctggag gggttcaatg acattgcaac aacaaataat aaaggcgctg ggcgcaaaac
     9001 cgcagattaa tgctgaagag gaaattcgtc gtagtgtcga ttttctgaaa agctacctgc
     9061 aaacttatcc gttcattaaa tcactggtgc tcgggatcag cggcggtcag gactccacgc
     9121 ttgccggaaa gctgtgccag atggcgatta atgagctgcg ccaggaaact ggcaacgaat
     9181 cactgcaatt tattgccgta cgcctgccct atggtgttca ggccgacgaa caagattgcc
     9241 aggatgccat tgcctttatt caaccggatc gcgtattaac cgttaatatc aagggcgcgg
     9301 tattggctag cgagcaggca ttgcgggaag caggcattga actgagcgat tttgtccgtg
     9361 gcaatgaaaa agcgcgtgag cggatgaaag cacaatatag cattgcgggt atgaccagcg
     9421 gtgtcgtggt gggcaccgat catgcagcag aagccattac cggattcttc actaaatatg
     9481 gtgacggcgg tacggacatt aatccgctgt atcgtctcaa caaacgtcag ggtaaacagt
     9541 tactggcggc attaggttgc ccggaacacc tttataagaa agcgccaacg gccgatctgg
     9601 aagatgatcg cccttctctg ccagatgaag tggcactcgg cgtgacctat gacaatatcg
     9661 acgactatct ggaagggaaa aacgtacctg aacaggtcgc cagaacaata gagaactggt
     9721 atctgaaaac cgaacataaa cgccgtccgc caattaccgt tttcgatgat ttctggaaaa
     9781 agtaataatt tgcacctatt ggattgtgcg aaaaagagta atttgttcac gccggatgcg
     9841 gcgtgaacgc cttattcgac ctacaaaact atgcaaattc aatacattgc aggagtcgaa
     9901 taggcctgac aggcgtagca cgtcagacgg tgtaaccttt gtcatcaacc cgcctctttt
     9961 ttaatcgcct cccgcctgtt acactggata gataaccagc attcggagtc aacagtggta
    10021 cggcgtttaa cttctccgcg gctcgaattt gaagctgcgg caatttatga atatcccgaa
    10081 catttacgtt cattccttaa tgacttaccc acccgaccag gggtgtatct gtttcatggc
    10141 gaaagtgaca ccatgccgct ctatatcggc aaaagcgtta atatccgcag tcgggtgctt
    10201 tctcatttac gcaccccgga tgaagccgcc atgctacggc aatcccggcg aattagctgg
    10261 atctgcaccg caggcgaaat cggcgctctg ctccttgaag cgcgattaat caaagaacaa
    10321 cagccgctgt ttaataaacg gttgcgccgc aatcgccagc tctgtgccct gcaattaaat
    10381 gaaaagcgcg tcgatgtggt gtatgccaaa gaggtggatt tttcacgagc ccccaacctg
    10441 tttggcctgt ttgccaatag gcgcgcagct ttgcaagcat tgcagagcat cgctgatgaa
    10501 caaaaacttt gttatggcct gttgggactg gaaccgttaa gtcgcggtcg tgcatgtttt
    10561 cgttcagcgc taaaacgttg cgccggagca tgctgcggta aagagagcca tgaggaacat
    10621 gcgctacgct tgcgccagtc tctggagcgt ttgcgggtgg tgtgttggcc ttggcaaggt
    10681 gcggtggcac tgaaagaaca gcacccggaa atgactcaat atcatattat tcaaaactgg
    10741 ctgtggctgg gggcggttaa ttcgctggaa gaagcgacaa cgttaattcg gacacccgcc
    10801 gggtttgatc acgacggtta taaaattctt tgtaagccgc tgctttccgg taactatgaa
    10861 attactgaac ttgatccggc gaatgaccag cgagccagtt gatttcactg aacaacagtt
    10921 taccttgcgg ttgtaaaagc cgcagggtgt gcctgccatc gaaccagcac gcgccctggt
    10981 cggtagtgag caatttatcg cccagttgcc acgccccgtt aatcacaaac accaccccgc
    11041 cgcgtgagcc aaaggtagta aacgtacgtt cggcaattct gactttcgcc ttacagacat
    11101 ccaggcgtgt catgatgttg aaatccatcg acatctgccc tgccgtcagt ttcgctttta
    11161 ccacctggtc cgctgcaaag gcaaaaggct gtaacggttt taaggtatgg ttaaagcggt
    11221 ctgcgctttc aaggaacatc tcgccgcctt ccagcaacgt cactatcctt tccatgccgg
    11281 gaaacaaaga aaactcccca ttcgccgcga tggatgcaat gctggcacgc cagtaaaaat
    11341 cacgttttgc cggtgggaaa gtgcaaattt cgcgcgtttc accggcagcg tttcgccaca
    11401 gattcaccga cattttacgc atatcaaagt attccatact cgctcccggc ccgttctttt
    11461 cattgttatc gttcaggtaa ccgtggatgg ttatcggcaa ctcgggtgat ttacgttagt
    11521 ggtgatcagg ctttttttac aactgtttga gaagagaaaa gaaaaccgcc gatcctgtcc
    11581 accgcattac tgcaaggtag tggacaagac cggcggtctt aagttttttg gctgaaagat
    11641 tattcagcag ttgcaggcat tttacctttt gccgctggac gttctgtcag acgcttctca
    11701 aaattagcat taaattgctt tttctgttcc ggcgtcagga tgttgtaaat tttgttctgg
    11761 gtttccatgt gcgccagcat gttagctttg cgctgttctt ccatttttgc gatctgcgct
    11821 tcagcttttg ctttatcgaa ggtatcgctg gcaatgatgt catgcattgc gcggcgttct
    11881 tccagcggcg gacgtttcat ctggtcacgc tggcctttca tgatttcgcg gatctgctgt
    11941 ttctgcgcgt cggtcaggtt caggtctttg aacatcatgt cctgatgcgg accgaacttg
    12001 cctttgtggt gcatcatcgg cttcgcgtca gccggtgctg cggtagtggt gtctgcggca
    12061 tgggccaggt tagccgcgcc aagagccagg gtagaggcaa caaacagtgc agttaattta
    12121 cgcatattct atatccttcc tttcagttat ttattacggc tttcttaagt agcgtgccgt
    12181 gttgacgaga ttaactttac tgactttagc gtcaattaat cagagcaacg gtaaaacaat
    12241 gaaagtgtaa aaaacacttt tgcgccaatt atggagaaaa aaagaaaatt tgatggagag
    12301 tgatgagaga ataatacaac acgatgattt tgcagagatt atgaagaact ataccggatg
    12361 actggtgata aataaagcaa ataaccagga ttaatctgta ttaatttata agaaagcaac
    12421 ttaatacccg cagaatgatt tctgcgggta agtattagct tattttttcg agcattaatc
    12481 ccgcgcgtaa tcccaacgct accaacggat tagggaataa cacatactct acatcatggg
    12541 ttacggtaaa acgttcctct ccgtcctgcg ccagcaatgt tcctttctca aacggcataa
    12601 aattcagcgt gtcacttgcc atatgcattt cgaaggacgg cgagtggcga gtaatttgcg
    12661 aaaccacccg ataacggagc ggcggtgttc tcacgatacc gacactctca ccagatagca
    12721 gcgcagcaat tgcgctggca gttactgcaa actggcgatg atcgttttgc ccaaagggca
    12781 acgctttgcc aagttccagc gtacaggcca gcgcgccaaa atgtctggcg ctgaaatggg
    12841 taaacgtacc accaggttcc tgatggaaca ccagcgcctc cagccccgcc gcacccagcc
    12901 acgtcagaaa tttctcgtcc cagggaatgt cgcgttgcgg taatacaccg aactgcggat
    12961 gcaaggagcc acgaattgcg gtatgtagat caaggtgcca gcgcacagat tctttgccca
    13021 ggtcataaaa atcttccagg cactgttcca gttcgcgcgc ccgacaggtt tctccgcttt
    13081 cagcaaatag ctgccaacga ccgccaaaca ttcgattcat atcgctatgg caataacgtt
    13141 tcccttgctt cagcgcagga ggattcccga ggatcaccag caaccgccaa cgtaacggga
    13201 tctcgccgtg agatatcgcg ccaagcaacg cgtccagcat ctccacaggt gccgtctcat
    13261 taccgtgtat tcccgctgaa atcaccagtg cgccttgcgg tggcgttaat ggcgtcagtt
    13321 ccagcacacc atcgcccagc cagcgccagc gaacgccgtt gatttctcgc tcggtgataa
    13381 ccggtttttt acccgttaag gtcagagcaa gaaaattatc cattgccccc tccctcgcgc
    13441 tggaacggat aaaccgaacc gagattcagt aattggctca atacatccag tgcttcccgc
    13501 ccttcgcgca gcaattgcgg gtcggccaga tcggcagcag taaggcgatc gcggtagtaa
    13561 cgatccaccc agtcattgag cgcattaaac agcgtatcgt tcatcatcac cgccggattc
    13621 accgcccggc gttcttcttc tgtcaatacc acccgcaacc gcaggcacgc cgggccgccg
    13681 ccattcgcca tgctttcacg taaatcaaag acttttagtt cgctaatcgg gttgtcagcg
    13741 gcaaggagtt cattgagata accccatact ccggcgtgtt cccgacactc ctgaggcagc
    13801 accaacatca tggaaccatc atcgcggctc aacagttggc tgttaaacag ataggtagac
    13861 accgcatccg acacggaaac ctgagttgcc ggaacttcta tcgccataaa accgttcacc
    13921 cgcgcacgca ggtttgccag taactgtgac tggcgagcga acgcctgttg gtggcaaaac
    13981 agcacctggc ggttactcac ggcaatcacg tcattatgaa aaacgccctg gtcgataacg
    14041 tccgggtttt gctgggcgaa aatcacctgt tggggattca cctgattcag ccttgccacc
    14101 gcctcgctgg cttcgcgagt ctgtcgcgcc ggataacggg aaggccgggt atcattgcct
    14161 tcttctcgcc cgtagacaaa aagttgcata cccggctcac cgtaatgacc gccgagacga
    14221 ttgtggtttg ccgccccctc atcaccgagc aacgctacct gcggcaacgc cgaatgaacg
    14281 ctaaatttct cttcgtcgtt aaaaatcgct tttaaaagtg attcggtgac gtgcgcttcc
    14341 agcgagcggt gaaatttatt gttcaggttg gcaacggtga gatgcacttt gccatccagc
    14401 gtatcggcag atggcgcgat cgttgccgca ttggctaccc acattggcga agcggaactg
    14461 acgctggaaa gccagtgcgg tgcctggcgt gcaacttttt ccagtacctg ctcatcgcta
    14521 ccactgaatc ccaactgacg cagcaccgga ataaacggac gctcgtgcgg cgggatcacg
    14581 gcctggggga atcccgcatc ggcaagggct ttcattttca gtaagccctg ttttgccgcc
    14641 tgtcgcgggt tagacacctg aaaacggtga cgggtagagg cttcattacc aaacgacagg
    14701 ccagcgtaat gatgcgtcag ccctaccagc ccgtcgaaat taacttccca ggcgttcatc
    14761 gcaccacctc atcggaaaaa tccagcccgg ggttaagcgt tgcgggcaaa gttaacgagt
    14821 ccgactccag gctcgccata ggccatgcgc agtaatctgc ggcataccag gcgctggggc
    14881 gatggttacc ggatgcacca atgccgccga atggcgcggt actggcagca ccagtaagcg
    14941 gtttgttcca gttaacaatc cccgcccgcg cctccagcaa cagttgatcg aatttttccc
    15001 gctcggggga aaccagaccg caagagagtc cgaagcgagt gttattcgcc attagaatcg
    15061 cttcctcgaa agaatcataa cgccagacgc gcaataacgg tccgaacacc tcttcatctg
    15121 gtacgccagc aacgcctgtc atttcaatga tccccggcgt cagcaacgat gtctctgatt
    15181 gtaataagcg cggcgcaagc agggttcgtc cgcccatcgc ttccagttgc tgccaggcag
    15241 taaccacctg ctgtgcggcc tgttcagaaa tcagcccgcc aataaacggc tgcggttcgt
    15301 catcccagtt gcccggcgtt aatcgctggc tgacggcaac cagacgagca agaaacgcat
    15361 cgccctgcgc tccgcttttg agcaataaac ggcgggcgca ggtgcagcgt tgcccggctg
    15421 tgacaaacgc cgactgaatg gtcagatgga cagccgcgtc gatatccgcc acctcatcga
    15481 taattagcgg attattaccg cccatctcaa gggcgagaat tttctccggc tgaccggaga
    15541 gctggcgatg cagctggtag ccggtattgg cgctaccggt aaacagcaaa ccgtcgagat
    15601 cctccagcgc actcagcgcc tgacccgttt cacgcccgcc ctgcaccagg ttcagtacgc
    15661 ccggcggcaa gccagcctgc tgccataaac gcattaccgc ttcgccactc cacggtgtca
    15721 gttcgctggg tttaaagata atggtgttac ctgccagcaa tgccggaacg atatgtccgt
    15781 tcggcaaatg accagggaaa ttatacggcc caaacaccgc cagcacgccg tgcgggcgat
    15841 gtcgcaggct cgccgcaccg tccggcattt cactacgctg ctcgccggta cgagcgtgat
    15901 acgccttaat tgatatcgcg attttattga tcatcgccgt cacttcggtt gccgcttccc
    15961 agcgcggctt gccggtttct cgggcaatga ttgcggttaa ttcgccttta ttcctttcca
    16021 gcaatccggc aaagcgttcg acaacgacct gacgttcagc caatgagagc cgcgcccagc
    16081 gcggaaacgc cgcacgggct gcccgacaag cctgctcgac ctgagcggca tcggcatcat
    16141 tgccttgcca taacacctcg cccgataccg gattacgctt cacacgcgat gcgccctggc
    16201 ccgttatcca gtcaccgtta atccataaag tcatgctgtt ttctcctctg cgcacaggcg
    16261 caccagacga acgcgatccc ctgcgtggca tttgagggca tccagttgtg cggcggttaa
    16321 aatcaaacgc tcggttgccg gatcggtacg cgccagcacc acgcggaaat ggtgataatt
    16381 ttcattggcg accaggcagg ccggaaaatc gccctgcgca ggctgccctt ctgccacttc
    16441 caccagccga cttttacgaa tggcgcgcac gcggtcgatg tcacactcaa gcgttggccc
    16501 gccgtcgaag atgtcgatat agttgcggta acgaaaacct tctttctcca gcaccgcgcg
    16561 ggcaggcgcg gtttgcggat gtacctgacc gatgacgtcc tgggcttcct gggataaaaa
    16621 gtgggtatag atcggatgtt tcggcatcag ttctgcaata aatgcctttt gcccggtgcc
    16681 gcagagaaaa tcggcgcggc taaaatccat cgaaaagaag cgtttaccga ggctttgcca
    16741 gaacggtgaa tagccgtgtt cgtcaatcac cccgcgcatt tcagcaacca ctttgtcatt
    16801 aaacttgtcg cgaaaagccg ccataaacat aaagcgcgat ttcgacagca aatagccgtt
    16861 gccctctttg cgccagtccg ggtcgagaaa cagcgtgcac agctcgctgc tgccggtgtg
    16921 atcgttactg agaaacagcg tcggcaatgc gttatagaca ttcagctctt ttaaggcgtg
    16981 aaccaacgtg ccgacgcgat agttgtacca gggatcgttc agcccaaccg ccacctcaat
    17041 ggcacaaatc cccgccacgg tgcctgtctc gctatcttcc agcacgaaca catagccctg
    17101 ctcacttttg ggcagttcgc cttgccaggt tttgattgcc ctttcgatac gcgccgaaag
    17161 cgtggcttca ttggcgggaa gcgacgtcag gccgccgccc gttttgctgg caagctgcat
    17221 cagcgccgag acatctgatc gctcaacggg acggatgacc atcatgatga acctcggcta
    17281 acaaagtgtt cgcaagcagc tgcaaagcga tccagtccgg tcgtcacctc ttcttcgctg
    17341 acattgagcg caggcgcaaa acgcaccacg ttgccacccg caatcagtac catcacgcct
    17401 gctttcgccg cttcctgaga gatctgtttc gcttgcccgg cgtaatcggc attcagtaca
    17461 cagccaatca gcaaacctaa gccgcgaact tcactgaaca aaccatagcg gtgattaata
    17521 gtattaagac gctcaacaaa ccagtcgtga cgctgtttaa cgccattaag catctctggt
    17581 gtgttgatga gctccagcac tttgcctgcc accgccgagg ccagcggatt accgccatag
    17641 gtggtgccat gagtgccaac ggtcatcacg cgggcgcact cttcggttgc caacaacgca
    17701 ccgaccggga aaccgccgcc cagcgctttg gcggtagtta acagatcagg cgtcacgccg
    17761 tagtgcatat aggcatacag ttccccggtg cgcccgacgc cggtttgtac ttcatcaaaa
    17821 atcaacagcg cattgtggcg gttacacaat tcacgcagac cttgtaaaaa cgcgttgctg
    17881 gctggcacca caccgccttc cccctggatg ggttcgacaa tcaccgcaca ggtagagtcg
    17941 tcaatcagcg cgctggcaga gttaatatcg ttatatgcag catgacgaat atccgccggc
    18001 agtggcgcaa aatcctgtga ataggctggc tgcccacccg cactgacagt aaacagcgtg
    18061 cgaccatgaa acgcattttt gaacgccacg atgccgctct tatggctgcc gtagcggtcg
    18121 tgagcgaatt tacgcgccag ttttagcgcc gcttcgttgg cttccgcacc ggagttacaa
    18181 aagaagacgc gatcggcaaa cgtggcgtcg atcaattttt tcgccagtcg cagtaccggc
    18241 tcgttggtgt aaccgttgcc ggtatgccag aacttactcg cctgttcgtt cagcgcttca
    18301 cgcagttccg gatgcgcatg gcccagcgcg ttcaccgcaa tgccacccgc gaagtcgata
    18361 tactctttcc cctgctgatc ccacaagcgc gaaccttcgc cacgtaccgg tataaagggt
    18421 gccggagcgt aaacaggtat catccattca tcaaagtttt cacgcgtaat tggctgagac
    18481 atagcgacct ctacagtaaa taattcgtta tttatatatt aacaataagt aatgtttgcg
    18541 ctgtaaatgt agattgcagg gttcgtgcca gccagtgata aaagtgcata aacggcggag
    18601 gctaactgga aatcaaggag ttataaccaa accatatgca tttaaagtgc atataaagtg
    18661 aatacgtttg cgatgtgggt gaataaaaag aataaaaaac gcaatgttat gcagaagtaa
    18721 aatataattc tggaattgtg atcattgacg aaatttactg gaaattactg cgccattctg
    18781 acgcagcgcg caccaaaagc gggcattttt tgcgccatcg ttgacatcat taacaaccat
    18841 cgatcaaatc acttaacaac aggcggtaag caacgcgaaa ttctgctacc atccacgcac
    18901 tctttatctg aataaatggc agcgactatg aaatttgtct cttttaatat caacggcctg
    18961 cgcgccagac ctcaccagct tgaagccatc gtcgaaaagc accaaccgga tgtgattggc
    19021 ctgcaggaga caaaagttca tgacgatatg tttccgctcg aagaggtggc gaagctcggc
    19081 tacaacgtgt tttatcacgg gcagaaaggc cattatggcg tggcgctgct gaccaaagag
    19141 acgccgattg ccgtgcgtcg cggctttccc ggtgacgacg aagaggcgca gcggcggatt
    19201 attatggcgg aaatcccctc actgctgggt aatgtcaccg tgatcaacgg ttacttcccg
    19261 cagggtgaaa gccgcgacca tccgataaaa ttcccggcaa aagcgcagtt ttatcagaat
    19321 ctgcaaaact acctggaaac cgaactcaaa cgtgataatc cggtactgat tatgggcgat
    19381 atgaatatca gccctacaga tctggatatc ggcattggcg aagaaaaccg taagcgctgg
    19441 ctgcgtaccg gtaaatgctc tttcctgccg gaagagcgcg aatggatgga caggctgatg
    19501 agctgggggt tggtcgatac cttccgccat gctaatccgc aaacagcaga tcgtttctca
    19561 tggtttgatt accgctcaaa aggttttgac gataaccgtg gtctgcgcat cgacctgctg
    19621 ctcgccagcc aaccgctggc agaatgttgc gtagaaaccg gcatcgacta tgaaattcgc
    19681 agcatggaaa aaccgtccga tcacgccccc gtctgggcga ccttccgccg ctaatttagc
    19741 agctctcctg gctcaaactg ggtcaggaga attaaccttg agaaaaatca acaaactgtc
    19801 agtaatgatt tgttgcctgc cgtcctttgt tataccgtca ctgcgttttt agttgtctga
    19861 ccacttctct attatcaagt ttgatatagg aaactccacg atgaacgctg agcgtaactt
    19921 tctgtttgcc tgtcttattt ttgcgctggt catttacgct atccacgctt tcggtttatt
    19981 cgatctgctc accgatttac cccacttaca gacactcatt cgccagagcg gattgttcgg
    20041 ttatagcctc tatattctgt tattcatcat tgccaccctc tttctgttac caggaagcat
    20101 actggtgatc gctggaggaa tagtttttgg cccgctctta gggacactac tctcattaat
    20161 tgccgcaacg ctggcctcct cgtgctcatt cctgctggcg cgctggctgg gacgtgattt
    20221 actgctgaaa tacgtgggcc atagccatac cttccaggcc attgaaaaag gcattgcgcg
    20281 taacggtatt gattttctta ttctgacccg cttaatcccg ttgtttcctt acaatattca
    20341 aaattacgct tacggattaa ccaccatcgc cttctggcct tataccctta tttcggcact
    20401 cacgaccctg cccggtattg ttatttatac cgtgatggca agcgatctcg ccaatgaagg
    20461 cattacgctg cgctttattt tacaactctg tctggcgggc ctggcgctgt ttattctcgt
    20521 ccagctcgca aaactctacg cccgacacaa acatgtggat ctgtctgctt cgcgccgcag
    20581 cccacttact cacccaaaaa atgaaggata gaacgatgtc gcaacattat tcagtgtcat
    20641 ggaaaaaagg actggctgca ctctgcttac tggctgttgc cgggcttagc ggctgcgatc
    20701 aaaaagagaa tgcggcggca aaagtggaat acgacggact ttcgaacagc caaccgctgc
    20761 gcgtcgatgc caataaccat acggtaacca tgctggtgca aattaatggt cgtttcctca
    20821 ccgacgacac tcgtcacggt attgtgttta aagatggctc caacggacat aaatcgctgt
    20881 ttatggctta tgcgaccccg aaagcatttt atgaagccct gaaagaggca ggtggtacgc
    20941 cgggcgaaaa catgacgatg gataataaag aaacgactca tgtcacaggc agcaaactgg
    21001 atatttcggt caactggcaa ggggcggcaa aagcgtattc cttcgatgaa gtgattgttg
    21061 acagtaatgg caagaaactg gacatgcgct ttggcggtaa tttaacggca gcagaagaga
    21121 agaaaacggg ttgcctggtg tgtctggata gctgcccggt cggcatcgtc agcaatgcaa
    21181 catacactta tggtgcggtg gaaaaacgcg gcgaagtgaa attcaaaggc aatgcctcag
    21241 ttctcccggc ggataacacg ctggcaacgg ttacctttaa aatcaccgaa taaagccagg
    21301 ataaaggatg atgaagatac aatcgcgtaa aatctggtac taccgtataa ccctcatcat
    21361 cctgttgttc gctatgctgc tcgcatgggc gctgcttccc ggcgtccatg agtttatcaa
    21421 tcgcagcgtt gcggcgtttg ccgccgtgga ccaacagggt atagaacgct ttattcagtc
    21481 ttacggcgca ctggcggcag ttgtctcgtt cttgttgatg attttacagg ccattgctgc
    21541 accgctacct gcgtttttga tcacctttgc caatgcgtcg ctgtttggcg cgttctgggg
    21601 cggcttgctg tcgtggacca gttcgatggc cggcgcggcg ctgtgctttt ttatcgccag
    21661 agtgatgggc cgcgaagtgg tggaaaaatt aaccggcaaa accgtgcttg acagtatgga
    21721 cggctttttc actcgctacg gcaaacacac catcctggtc tgtcggttat tgccttttgt
    21781 ccctttcgat ccaatcagct atgctgccgg tctgacttca atacgttttc gctcgttttt
    21841 tatcgccacc gggcttggtc aattaccggc gactattgtt tattcctggg cgggcagcat
    21901 gttaacaggc ggtactttct ggtttgtcac cggactgttt attctgtttg ccctgaccgt
    21961 ggtgattttt atggcgaaga aaatatggct tgagcgccag aagaggaatg cctgatgggt
    22021 ttaccgccgc ttagcaaaat tcctttcatt ttacgtccgc aggcgtggct gcatcgtcgc
    22081 cattacggcg aggtgctaag cccgattcgc tggtgggggc ggatcccgtt tatcttttat
    22141 ttggtgtcaa tgtttgttgg gtggctggag cgcaaacgct caccgctcga tccggtagtg
    22201 cgatcgcttg tcagcgcgcg cattgcgcaa atgtgcctgt gtgagttttg cgtggatatc
    22261 accagtatga aagtcgccga gcgtaccggc agcaccgata aactgctggc agtggctgac
    22321 tggcggcaaa gcccgctctt cagcgatgaa gaacggctgg cgctggagta cgccgaagcc
    22381 gcaagcgtaa cgccgccaac ggtcgatgat gccctgcgca cccgactggc tgcgcatttt
    22441 gacgctcagg cgctcaccga actgacggca ttgatcggcc tgcaaaacct gtcggctcgt
    22501 tttaattctg ccatggacat tcccgctcag gggctgtgcc gtattccgga aaaacgttct
    22561 taaggagaga agatgcgcca ttgtgggtgg ttgctgggat tgttatcgct gttttctctg
    22621 gcaacacatg ccagtgactg gcaggaaatt aaaaatgagg ccaaagggca aaccgtctgg
    22681 tttaacgcct ggggcggcga taccgcaatt aaccgctatc tcgactgggt tagcggcgag
    22741 atgaaaaccc attacgctat aaacctgaag attgttcgtc tggcggatgc cgcagacgcg
    22801 gtgaagcgca ttcagaccga agctgctgcc ggacgtaaaa cgggcggctc ggtggatctg
    22861 ctctgggtga acggcgaaaa cttccgcacc ttaaaagagg ccaatttatt acaaacgggc
    22921 tgggcggaga ctctgcccaa ctggcgctat gtcgacacac agctgccggt gcgggaagat
    22981 ttttcagtgc ctacagaagg ggctgaatcg ccctggggcg gcgcacaact gacgtttatc
    23041 gcccgccgcg atgttacgcc acagccacca caaacgccgc aagccttact ggagtttgct
    23101 aaagccaatc ccggcacggt tacctatccg cgcccaccgg actttaccgg cacggcgttt
    23161 cttgaacagt tgctgattat gctgacgccc gatcccgccg cgttaaaagt tgccccggat
    23221 aaagccactt tcgcccgcgt cactgcgccc ttgtggcaat atcttgacgc gctacatccc
    23281 tatttatggc gcaaaggaaa ggatttcccg ccttcgcccg cgcggatgga tgctctgctg
    23341 aaagccggca cattgcgcct gtcgctgacc tttaaccctg ctcatgccca gcaaaaaatc
    23401 gccagcggcg atttgcctgc aagcagttac agttttggtt ttaaccaggg gatgattggc
    23461 aacgtgcatt tcgtcaccat tcctgctaac gcaaatgcca gtgctgcggc gaaggtggtt
    23521 gccaacttcc tgctctcacc caatgcgcaa ctgcgtaaag cggatcccgc tgtctggggc
    23581 gatccttctg ttctcgatcc gcaaaaactg cctgatgggc agcgcgaaac attgcaatca
    23641 agaatgccgc aagatctgcc gccggtactg gctgaaccgc acgcaggttg ggtaaatgcg
    23701 ctggaacaag aatggctaca ccgttacggt acgcattaat ctttttgctg tgggcgatgg
    23761 tggcggtgat ttatgcacca ctgatcccgg cagctctcac gctgatatcg cctgctttgt
    23821 cgttgacaca ctggcaggcg ttatttgccg atccacagtt accgcaggca ttactggcaa
    23881 cgctggtgtc gacaaccatc gcggcggtcg gggcgttgtt gattgccctg ctggtgattg
    23941 tggcgctgtg gccttggccg aaatggcagc gtatgtgtgc ccgtctgccg tggctgctcg
    24001 ccattcccca tgtggctttt gccaccagcg cccttctgct ctttgctgag ggagggctgc
    24061 tttatgacta tttcccgtat ttcactccgc taatggacaa attgggtatt gggctgggcc
    24121 tcacccttgc agtgaaagaa agcgcatttc tgctgtggat cttagcggca gtattgagcg
    24181 aaaaacggct gttgcagcag gtcattgtgc tggattcgct gggctacagc cgctggcaat
    24241 gcctgaactg gctgctcttg ccctccgtcg cacctgcact ggcaatggcg atgttggcga
    24301 ttgttgcctg gtcgctttcg gtcgtggatg tggcgattat tcttgggcca ggtaatccac
    24361 cgacgctggc agtaattagc tggcagtggt taactcaggg cgacgcggat caacaaacga
    24421 aaggcgcact cgccagtctg ctgctgatgc tgttactcgc cgcctacgtt ttgctgagct
    24481 atctgctatg gcgcagctgg cggcgcacta ttccccgcgt agatggcatt cgcaagcctg
    24541 ccacaccttt attaccaggc actacgctgg cgagtttttt acccttaacc ggtgtgctgt
    24601 gtgtggttct gctggcgatc ctcgcggatc agtcgacgat caatagtgaa gcactcatca
    24661 acagcctgac aatggggctg gtggcgacat tcatcgcttt gctcctgtta ctgctgtggc
    24721 tggaatgggg gccacagcgt cgccagttgt ggctctggtt gcccattcta ttgcccgctc
    24781 tgccactggt ggcgggccag tacacgctgg cgctatggct gaacctggat ggaagctgga
    24841 cggcggtggt ctgggggcat ctgctgtggg taatgccgtg gatgctgttt atcctgcaac
    24901 ccgcctggca gcgcattgat tcacggttaa ttttgattgc gcaaacactg agctggtcgc
    24961 gggccaaaat cttcttttac gtgaaatgtc cgcttatgct acgccctacg ctgattgcct
    25021 tcgcggtggg attttcagtc agtattgcgc agtatatgcc gacgctgtgg ctgggcgcgg
    25081 ggcgttttcc gacgctcacc actgaggcgg tggcattaag cagcggcggc agcaatggta
    25141 ttttcgccgc ccaggcttta tggcaactgc tattaccgct tattattttt gccctgaccg
    25201 cgttagtcgc aaaatgggta ggttatgtca gacaaggact ccgctaatgc tctgcgtgaa
    25261 aaatgtttcg ctacgtttac cagaaagccg cttgctgaca aacgttaact ttacggtgga
    25321 taaaggtgac attgtcacgt taatggggcc gtctggctgt ggaaaatcca ctctgttttc
    25381 atggatgatt ggtgcactgg ctggacagtt ttcttgtaca ggtgagctat ggctcaatga
    25441 gcaacggatt gacatgctac ccaccgcaca gcgtcagatt ggcattcttt tccaggatgc
    25501 actgttattt gaccagttca gcgtcgggca aaatttactg ctggcgctac cgtcgacgct
    25561 caaggggact gccaggcgca atgccgtgaa agacgcgctt gatcgagcgg gactggctga
    25621 aacttatcac caggaccctg cgacattatc aggcggtcag cgagcgcggg tggccctgct
    25681 gcgcgccctt ctcgctcagc caaaagcgtt actcctggat gagccattca gccgtcttga
    25741 tgtggctctg cgcgataatt ttcgccagtg ggtgttcagc gaagttcgcg aactggcgat
    25801 ccccgtcgtt caggtaacgc acgatctcca ggatgttcct gctgatagtt ctgttctgga
    25861 tatggcgcag tggtcagaaa attacaacaa actgcgataa cgcaaagttt ttctcaatgc
    25921 gtcagttcag aatggcgcac tcaaaactac aatgtcggga ttttcgatga aacgtgtttc
    25981 tcaaatgacc gcgctggcaa tggctttagg gctggcttgc gcttcttcgt gggccgctga
    26041 actggcgaag cctcttacac ctgaccagct tcaacaacaa aatggcaaag cgatagatac
    26101 tcgccccagc gcgttttata acggctggcc acaaacctta aatggccctt ctggtcatga
    26161 acctgccgcc ttaaacctct ctgccagctg gctcgacaaa atgagcaccg aacagctcaa
    26221 cgcgtggatc aagcaacata acctgaaagc cgatgccccg gtggcgctgt acggtaatga
    26281 caaagatgtc gacgccgtca aaacgcgact gcaaaaagca ggttttacgc atatctccat
    26341 tctgagtgac gcgctaagcg aaccttcccg tctacaaaaa ctgccgcact ttgagcagct
    26401 ggtttatccg caatggctgc acgacctgca acaaggtaaa gaggttacgg cgaaacctgc
    26461 cggtgactgg aaagtcattg aagcggcctg gggcgctcct aagctttacc ttatcagcca
    26521 tattcccggt gctgactaca tcgataccaa cgaagtggaa agcgaaccgc tgtggaacaa
    26581 agtttctgat gaacaactaa aagcgatgct ggcaaaacac ggcattcgcc atgacaccac
    26641 ggtcattctg tacgggcgtg acgtatacgc tggagcgcgt gtggcgcaaa ttatgctcta
    26701 cgcaggcgtg aaagatgtgc gtctgctgga tggcggctgg caaacctggt ccgacgcggg
    26761 actgcctgtt gagcgaggaa cgccaccgaa agtgaaagcg gaaccggatt tcggcgtgaa
    26821 gatcccggca caaccgcagt tgatgctcga tatggaacag gcccgtggac tgctgcatcg
    26881 ccaggatgca tcgctggtga gcattcgttc gtggccagaa tttatcggta cgaccagcgg
    26941 ttacagctat attaaaccaa aaggtgaaat agccggagca cgttggggac acgctggtag
    27001 cgactcgacg catatggaag attttcataa cccggatggc accatgcgca gcgccgatga
    27061 tattaccgct atgtggaaag cgtggaatat caaaccagag cagcaagttt cattctactg
    27121 cggcaccggc tggcgcgcgt ccgaaacctt tatgtacgca cgcgccatgg gttggaagaa
    27181 tgtttccgtg tatgacggcg gctggtacga atggagcagc gatccaaaaa atccggtagc
    27241 aaccggtgaa cgcggcccgg acagtagcaa ataacattgc aattactgac gctggtgcga
    27301 cttcagcgtc agatacccac tccacacccg cgtaaaggtc gtcatccagc acaacgcacc
    27361 gaagatccac gcaaaccacg caaaccatgc agggaataaa catcccagca caaacagtaa
    27421 gatcgtttct gtgccttcgg ttaacccgcc cagataataa aacgatttat gcgcataacc
    27481 gggattatca atctgatgtt tcgccgccag cgcagcaaaa gcgagaaaac tgctgcctgt
    27541 gccgataaac gcaaacagca gccagccgcc tgccagggcg ttttgctgcg gtgcggcgag
    27601 gataaaccca aaaggtacca gcgcgtaaaa aagaaaatcg agagaaatat caagaaagcc
    27661 gcccgcgtcg gtaagccctc tgcgccgcgc cagcgcaccg tccagaccat caagcagcct
    27721 gttcaacaaa atgacgacaa gcgccgccag ataccagccc agcgccagaa aaggcagtgc
    27781 cagcacgccg atggcaaatc ccactaatgt taaaccatct ggcgtaatgc ccggtttatc
    27841 aagaacccgc acgcactgat gcaacaacgg tttaatccgg ggatgaagat ggcggtctag
    27901 cacaggcact ccttaaatat aaagcctttc tgattgagca acagtgcgga tattatggca
    27961 tttttcgctt atctgcccgt gtgtaattta tgaaaatgat tgaagttgtt gccgccatca
    28021 ttgaacgtga tggcaaaatt ttactcgcgc aacgccccgc ccagagcgat caggcgggat
    28081 tatgggagtt tgccggtggt aaagtcgagc cggatgaaag ccagcggcag gcgctggtgc
    28141 gtgagttacg cgaagaactg ggcatcgaag caactgtggg tgagtatgtt accagccatc
    28201 agcgagaagt ttcgggccgg attatccatc ttcatgcctg gcacgtaccc gacttccacg
    28261 ggacgttaca ggcacatgaa catcaggcgc tggtctggtg ctcacctgaa gaggcgctgc
    28321 aatatccgct ggcccctgct gacattccat tattagaggc gtttatggct ttacgcgccg
    28381 ccagaccagc ggattagtgc taagggtttt gtcatcccgc tggcattgca gcagtattcc
    28441 ctcggcttta attaccgcgc cttcagaata atttttatcc tgataaacgc agcactgagt
    28501 acagggctgc gctgactgcc cgcctgaact gaacacttct ggcggtacgt ttacctccac
    28561 gtccggacga taatgcgggt tagccagtgc aattaatgga aatgctaata ctacggcgaa
    28621 caatgctcga ctcacaggga actccttaac gttattgtct ctgctactga taacggtagc
    28681 cgggtggcaa aactttagcg tctgagttat cgcatttggt tatgagatta ctctcgttat
    28741 taatttgctt tcctgggtca tttttttctt gcttaccgtc acattcttga tggtatagtc
    28801 gaaaactgca aaagcacatc acataaacaa cataagcaca atcgtattaa tatataaggg
    28861 ttttatatct atggatcaga catattctct ggagtcattc ctcaaccatg tccaaaagcg
    28921 cgacccgaat caaaccgagt tcgcgcaagc cgttcgtgaa gtaatgacca cactctggcc
    28981 ttttcttgaa caaaatccaa aatatcgcca gatgtcatta ctggagcgtc tggttgaacc
    29041 ggagcgcgtg atccagtttc gcgtggtatg ggttgatgat cgcaaccagg tacaggtcaa
    29101 ccgtgcatgg cgtgtgcagt tcagctcagc catcggcccg tacaaaggcg gtatgcgctt
    29161 ccatccgtca gttaacctct ccattctcaa attcctcggc ttcgaacaaa ccttcaaaaa
    29221 tgccctgact accctgccga tgggcggtgg taaaggcggt agcgattttg acccgaaagg
    29281 taaaagcgaa ggcgaagtga tgcgtttctg ccaggcgctg atgaccgagc tttatcgtca
    29341 tctgggcgcg gataccgacg ttccggcagg tgatatcggc gttggtggtc gtgaagtcgg
    29401 ctttatggcg gggatgatga aaaagctctc caacaatacc gcctgcgtct tcaccggtaa
    29461 gggcctttca tttggcggta gtcttattcg cccggaagct accggctacg gtctggttta
    29521 tttcacagaa gcaatgttaa aacgccacgg tatgggtttt gaagggatgc gcgtttccgt
    29581 ttctggctcc ggcaacgtcg cccagtacgc tatcgaaaaa gcgatggaat ttggtgctcg
    29641 tgtgatcact gcgtcagact ccagcggcac tgtagttgat gaaagcggat tcacgaaaga
    29701 gaaactggca cgtcttatcg aaatcaaagc cagccgcgat ggtcgagtgg cagattacgc
    29761 caaagaattt ggtctggtct atctcgaagg ccaacagccg tggtctgtac cggttgatat
    29821 cgccctgcct tgcgccaccc agaacgaact ggatgttgac gccgcgcatc agcttatcgc
    29881 caatggcgtt aaagccgtcg ccgaaggggc aaatatgccg accaccatcg aagcgactga
    29941 actgttccag caggcaggcg tactgtttgc accgggtaaa gcggctaatg ctggcggtgt
    30001 agcaacgtca ggccttgaaa tggcgcaaaa cgctgcgcgc ctgggctgga aagccgagaa
    30061 agttgacgca cgtttgcatc acatcatgct ggatatccac catgcctgtg ttgagcatgg
    30121 tggtgaaggt gagcaaacca actacgtgca gggcgcgaac attgccggtt ttgtgaaggt
    30181 tgccgatgcg atgctggcgc agggtgtgat ttaagttgta aatgcctgat ggcgctacgc
    30241 ttatcaggcc tacaaatggg cacaattcat tgcagttacg ctctaatgta ggccgggcaa
    30301 gcgcagcgcc cccggcaaaa tttcaggcgt ttatgagtat ttaacggatg atgctcccca
    30361 gggaacattt cttatgggcc aacggcattt cttactgtag tgctcccaaa actgcttgtc
    30421 gtaacgataa cacgcttcaa gttcagcatc cgttaacttt ctgcgatagc agcagatatg
    30481 ccagtaaaga aatcccatta gactattttt ttgataatct tcttcgcttt cgaacaactc
    30541 gtgcgccttt cgagaagcaa gcattatata atgccaggcc agttcttctt caattgtccc
    30601 gttttgaaaa gctgtgcttg atatcgagat catccatgat aattccgccg cccatattag
    30661 cttcgccgag gatttaccgg agcgattatt agcgcaatca gagatatagt ctgagggaaa
    30721 aacagcaaat ttattcaaca aggcgataac ctgctctggg gcttcctcca tgtttgcttt
    30781 aaaggtattg gctccatggt cgccagaaag aaaatgctcc attaaggcac aataactttc
    30841 gctatcttcg ataccccatt gatcctctaa agactcgcgt cttttactta tgatatcgat
    30901 cgagtcaaaa ggaagcacat gatattggaa ggtatctttg ccaggttcag gctttcgcgg
    30961 ccagaactcc agcgtttcag accattgctt atgatagaat cgataaggtg cgatcaattg
    31021 tagcgcctgt aacttctcga tactgagcgg ctcaatacct ttagcctgat aataatgcag
    31081 ttgttctttt tttgctttaa aaccggcccg aacaataagc cccatcataa ttaatagata
    31141 aagaaaagag catcccgcag taatcaggcc tctttcattc aaaccgttgg atgttatcgc
    31201 tgcgaa
//
LOCUS       DAZFQD010000051        31037 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.051, whole genome shotgun sequence.
ACCESSION   DAZFQD010000051 DAZFQD010000000
VERSION     DAZFQD010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31037
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.051"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(139..2025)
                     /gene="prpE"
                     /locus_tag="ACHKTW_002338"
     CDS             complement(139..2025)
                     /gene="prpE"
                     /locus_tag="ACHKTW_002338"
                     /EC_number="6.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050218 - propionate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate--CoA ligase"
                     /protein_id="HFO6463350.1"
                     /translation="MSFSEFYQRSINEPEQFWAEQARRIDWQTPFTQTLDHSNPPFAR
                     WFCEGRTNLCHNAIDRWLEKQPEALALIAVSSETEEERTFTFRQLHDEVNAVASMLRS
                     LGVQRGDRVLVYMPMIAEAHITLLACARIGAIHSVVFGGFASHSVAARIDDAKPVLIV
                     SADAGARGGKIIPYKKLLDDAISQAQHQPRHVLLVDRGLAKMARVSGRDVDFASLRHQ
                     HIGARVPVAWLESNETSCILYTSGTTGKPKGVQRDVGGYAVALATSMDTIFGGKAGGV
                     FFCASDIGWVVGHSYIVYAPLLAGMATIVYEGLPTWPDCGVWWKIVEKYQVSRMFSAP
                     TAIRVLKKFPTAEIRKHDLSSLEVLYLAGEPLDEPTASWVSNTLDVPVIDNYWQTESG
                     WPIMAIARGLDDRPTRLGSPGVPMYGYNVQLLNEVTGEPCGVNEKGMLVVEGPLPPGC
                     IQTIWGDDDRFVKTYWSLFSRPVYATFDWGIRDADGYHFILGRTDDVINVAGHRLGTR
                     EIEESISSHPGVAEVAVVGVKDALKGQVAVAFVIPKESDSLEDRDVAHSQEKAIMALV
                     DSQIGNFGRPAHVWFVSQLPKTRSGKMLRRTIQAICEGRDPGDLTTIDDPASLDQIRQ
                     AMEE"
     gene            complement(2065..3516)
                     /gene="prpD"
                     /locus_tag="ACHKTW_002339"
     CDS             complement(2065..3516)
                     /gene="prpD"
                     /locus_tag="ACHKTW_002339"
                     /EC_number="4.2.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047547 - 2-methylcitrate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019679 - propionate metabolic process,
                     methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate dehydratase"
                     /protein_id="HFO6463351.1"
                     /translation="MSAQINNIRPEFDREIVDIVDYVMNYEISSKVAYDTAHYCLLDT
                     LGCGLEALEYPACKKLLGPIVPGTVVPNGVRVPGTQFQLDPVQAAFNIGAMIRWLDFN
                     DTWLAAEWGHPSDNLGGILATADWLSRNAVASGKAPLTMKQVLTGMIKAHEIQGCIAL
                     ENSFNRVGLDHVLLVKVASTAVVAEMLGLTREEILNAVSLAWVDGQSLRTYRHAPNTG
                     TRKSWAAGDATSRAVRLALMAKTGEMGYPSALTAPVWGFYDVSFKGESFRFQRPYGSY
                     VMENVLFKISFPAEFHSQTAVEAAMTLYEQMQAAGKTAADIEKVTIRTHEACIRIIDK
                     KGPLNNPADRDHCIQYMVAIPLLFGRLTAADYEDNVAQDKRIDALREKINCFEDPTFT
                     ADYHDPEKRAIANAITLEFTDGTRFEEVVVEYPIGHARRRQDGIPKLVDKFKINLARQ
                     FPTRQQQRILEVSLDRARLEQMPVNEYLDLYVI"
     gene            complement(3550..4719)
                     /gene="prpC"
                     /locus_tag="ACHKTW_002340"
     CDS             complement(3550..4719)
                     /gene="prpC"
                     /locus_tag="ACHKTW_002340"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050440 - 2-methylcitrate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HFO6463352.1"
                     /translation="MSDTTILQNSTHVIKPKKSVALSGVPAGNTALCTVGKSGNDLHY
                     RGYDILDLAEHCEFEEVAHLLIHGKLPTRDELAAYKTKLKALRGLPANVRTVLEALPA
                     ASHPMDVMRTGVSALGCTLPEKEGHTVSGARDIADKLLASLSSILLYWYHYSHNGERI
                     QPETDDDSIGGHFLHLLHGEKPSQSWEKAMHISLVLYAEHEFNASTFTSRVIAGTGSD
                     MYSAIIGAIGALRGPKHGGANEVSLEIQQRYETPDEAEADIRKRVENKEVVIGFGHPV
                     YTIADPRHQVIKRVAKQLSQEGGSLKMYNIADRLETVMWESKKMFPNLDWFSAVSYNM
                     MGVPTEMFTPLFVIARVTGWAAHIIEQRQDNKIIRPSANYVGPEDRQFVALDKRQ"
     gene            complement(4764..5654)
                     /gene="prpB"
                     /locus_tag="ACHKTW_002341"
     CDS             complement(4764..5654)
                     /gene="prpB"
                     /locus_tag="ACHKTW_002341"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046421 - methylisocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HFO6463353.1"
                     /translation="MSLHSPGKAFRAALSKENPLQIVGTINANHALLAQRAGYQAIYL
                     SGGGVAAGSLGLPDLGISTLDDVLTDIRRITDVCSLPLLVDADIGFGSSAFNVARTVK
                     SMIKAGAAGLHIEDQVGAKRCGHRPNKAIVSKEEMVDRIRAAVDAKTDPDFVIMARTD
                     ALAVEGLDAAIERAQAYVEAGAEMLFPEAITELAMYRQFADAVQVPILANITEFGATP
                     LFTTDELRSAHVAMALYPLSAFRAMNRAAEHVYNVLRQEGTQKSVIDTMQTRNELYES
                     INYYQYEEKLDDLFARSQVK"
     gene            5893..7479
                     /gene="prpR"
                     /locus_tag="ACHKTW_002342"
     CDS             5893..7479
                     /gene="prpR"
                     /locus_tag="ACHKTW_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate catabolism operon regulatory protein
                     PrpR"
                     /protein_id="HFO6463354.1"
                     /translation="MAHPPRLNDDKPVIWTVSVTRLFELFRDISLEFDHLANITPIQL
                     GFEKAVTYIRKKLANERCDAVIAAGSNGAYLKSRLSVPVILIKPSGYDVLQALAKAGK
                     LTSSIGVVTYQETIPALVAFQKTFNLRLDQRSYITEEDARGQINELKANGTEAVVGAG
                     LITDLAEEAGMTGIFIYSAVTVRQAFSDALDMTRMSLRHNTHDATRNALRTRYVLGDM
                     LGQSPQMEQVRQTILLYARSSAAVLIEGETGTGKELAAQAIHREYFARHDARQGKKSH
                     PFVAVNCGAIAESLLEAELFGYEEGAFTGSRRGGRAGLFEIAHGGTLFLDEIGEMPLP
                     LQTRLLRVLEEKEVTRVGGHQPVPVDVRVISATHCNLEEDMRQGQFRRDLFYRLSILR
                     LQLPPLRERVADILPLAESFLKVSLAALSAPFSAALRQGLQASETVLVHYDWPGNIRE
                     LRNMMERLALFLSVEPTPDLTPQFMQLLLPELARESAKIPAPRLLTPQQALEKFKGDK
                     TAAANYLGISRTTFWRRLKN"
     gene            complement(7580..7855)
                     /gene="yahO"
                     /locus_tag="ACHKTW_002343"
     CDS             complement(7580..7855)
                     /gene="yahO"
                     /locus_tag="ACHKTW_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family periplasmic protein YahO"
                     /protein_id="HFO6463355.1"
                     /translation="MKIISKMLVGALAFAVTNVYAAELMTKAEFEKVESQYEKIGDIS
                     TSNEMSTADAKEDLIKKADEKGADVLVLTSGQTDNKIHGTANIYKKK"
     gene            8002..8673
                     /gene="yahN"
                     /locus_tag="ACHKTW_002344"
     CDS             8002..8673
                     /gene="yahN"
                     /locus_tag="ACHKTW_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HFO6463356.1"
                     /translation="MMKLLHLFMDEITMDPLHAVYLTVGLFVITFFNPGANLFVVVQT
                     SLASGRRAGVLTGLGVALGDAFYSGLGLFGLATLITQCEEIFSLIRIVGGAYLLWFAW
                     CSMRRQSTPQMSTLQQPISAPWYVFFRRGLITDLSNPQTVLFFISIFSVTLNAETPTW
                     ARLMAWAGIVLASIIWRVFLSQAFSLPAVRRAYGRMQRVASRVIGAIIGVFALRLIYE
                     GVTQR"
     gene            complement(8690..8935)
                     /gene="yahM"
                     /locus_tag="ACHKTW_002345"
     CDS             complement(8690..8935)
                     /gene="yahM"
                     /locus_tag="ACHKTW_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YahM"
                     /protein_id="HFO6463357.1"
                     /translation="MAVQLFKTLLNQIPLLSSLQSGTLPLFGYSGRGRPMKKAHTGES
                     GLKWEAKDSSKLIGNKDHALRGLSSPMAGIRQIRLIT"
     gene            complement(9348..10163)
                     /gene="yahL"
                     /locus_tag="ACHKTW_002346"
     CDS             complement(9348..10163)
                     /gene="yahL"
                     /locus_tag="ACHKTW_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YahL"
                     /protein_id="HFO6463358.1"
                     /translation="MISLKAPHNNLMPYTQQSILNTVKNNQLPEDIKSSLVSCVDIFK
                     VLIKQYYDYPYDCRDDLVDDDKLIHLMAAVRDCEWLDDNALTINVQFNDFPGFYDWMD
                     YPDHPVKFVFRILENQKGTVWVYDQDDAFLDIKANVQAGRFTGLKKLVQFIDSVRTDC
                     KCILLEHHMPLLRIFPKGKECMRVEKWLREMSSIPETDAPIKQALAHGLLLHLKNIHP
                     VFPESLVMLLLSVLDVKTYRDDARLNEWISNRVQELGDRYYPVNKHVKIRYTL"
     gene            complement(10406..11455)
                     /gene="yahK"
                     /locus_tag="ACHKTW_002347"
     CDS             complement(10406..11455)
                     /gene="yahK"
                     /locus_tag="ACHKTW_002347"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase YahK"
                     /protein_id="HFO6463359.1"
                     /translation="MKIKAVGAYSAKQPLEPMDITRREPGPNDVKIEIAYCGVCHSDL
                     HQVRSEWAGTVYPCVPGHEIVGRVVAVGDQVEKHAPGDLVGVGCIVDSCKHCEECEDG
                     LENYCDHMTGTYNSPTPDEPGHTLGGYSQQIVVHERYVLRIRHPQEQLAAVAPLLCAG
                     ITTYSPLRHWQAGPGKKVGVVGIGGLGHMGIKLAHAMGAHVVAFTTSEAKREAAKALG
                     ADEVVNSRNADEMAAHLKSFDFILNTVAAPHNLDDFTTLLKRDGTMTLVGAPATPHKS
                     PEVFNLIMKRRAIAGSMIGGIPETQEMLDFCAEHGIVADIEMIRADQINEAYERMLRG
                     DVKYRFVIDNRTLTD"
     gene            complement(11542..12498)
                     /locus_tag="ACHKTW_002348"
     CDS             complement(11542..12498)
                     /locus_tag="ACHKTW_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO6463360.1"
                     /translation="MKKSWRNNVEFYLIGLLVLTVAAFSITMPEIFWSISNFQSVASQ
                     MPVLGILALAMAVTMLCGGINLSIIATANACSLVMAWVATQYPPGIATVVATLLAGAG
                     AAVIIGLCNGVLIAGIRVSPILATLGMMTLLKGINILVTGGSAIANYPSWVLWLNHAQ
                     WFGIPLPMWLFAAVALGLWILLEKTPLGKAIYLIGSNERATLYSGINTRRVLIWVYVI
                     SALLCAVAAFLMMSKLNSAKASYGESYLLVSILAAVLGGVNPDGGSGRIIGMVLALFL
                     LQIIESGFNILGISPYLTMALWGTLLLCFIQVRGMLGLDRVV"
     gene            complement(12495..13466)
                     /locus_tag="ACHKTW_002349"
     CDS             complement(12495..13466)
                     /locus_tag="ACHKTW_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO6463361.1"
                     /translation="MAELKKRHEFWLALLIVVLFVGLAWRSDEFLTFGNLYDLANNYA
                     MLTILACGLFVVLISGGIDISFPAMTIIAQYGMVLLLQKIGGNFAVAFALAGCIGILL
                     GLINALLVNRLRVPSIIITISTLNIFYGLLLWLSKGVWLYDFPPWFEQGVMLFKYTDA
                     DGYDYGLGLPLIAMITVVLLTAFIMNFTSVGRKIYALGGNRESASRIGFSVLKLQLFV
                     YGYMGLMSGAAGVVQSWTVMTVAPDSLLGYELTVLAAVVLGGTSLLGGRGTLTGTLLG
                     VVLLALMQNGLNLLGVSSYWQTLITGIIIVASISATAWSQHQNRSLL"
     gene            complement(13459..14943)
                     /locus_tag="ACHKTW_002350"
     CDS             complement(13459..14943)
                     /locus_tag="ACHKTW_002350"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HFO6463362.1"
                     /translation="METFLSLRHINKTFHATRALRDVSLDFMSGEVHCLAGQNGCGKS
                     TLIKIMSGVYRPDEGAEITLGGKNWSKLTPAASVAQGIQVIYQDLSLFPNLSVWENIA
                     VNHYHHGLFVNRRRLREVAQAAMTSINVTLPLDTLVSELSIARCQLVAICRALAQDAR
                     LIVMDEPTASLTHQEVQGLLQVVHQLRERGICVVFVSHRLEEVMEVSDRISVLKDGEL
                     VGTFPAAEMTTKQLGFLMTGQEFEYQVRELWQGKSSTPVLEVRNLSRHGEYLNINLRV
                     EAGEVVSIVGLLGAGRTELCLSLFGMTRPDAGEILINGQPVTLHSNQDAIRHGIGYVS
                     EDRMSRGLVMAQSIEDNIISTVFHKVKDRFGFLSETKVRDLVDRLIKALTIKVSDPHL
                     PVNTLSGGNAQRVSIAKWLAIGPRLLILDSPTVGVDIANKAGIYGIISDLAAHGIAVL
                     MICDEIEEAWYQSHRILVMQKGQITHSFLPDSSSQARIAEVVNG"
     gene            complement(14992..15975)
                     /locus_tag="ACHKTW_002351"
     CDS             complement(14992..15975)
                     /locus_tag="ACHKTW_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO6463363.1"
                     /translation="MNKRFVINMVSSLLLGAALISAPLQAAEKVVVNISKVDGMPWFN
                     RMGEGVVESGKAFGVNASQVGPSSTDAPQQVKIIEDLIARKVNAITIVPNDANVLEPV
                     FKKARDAGIVVLTNESPGQPSANWDIEIIDNEKFAAEYVEHMAKRMGGKGGYVIYVGS
                     LTVPQHNLWADLLVKYQKEHYPDMHEVTRRMPVAESVDDSRRTTLDLMKTYPDLKAVV
                     SFGSNGPIGAGRAVKEKRAKNKVAVYGMMIPSQAASLIKSGDITEGITYDPASAGYAL
                     AAVASTLLKGEEIKPGLEMQNLGKADVDMDKRIIRFHKVLLVNKDNIDSLY"
     gene            complement(16229..16639)
                     /locus_tag="ACHKTW_002352"
     CDS             complement(16229..16639)
                     /locus_tag="ACHKTW_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HFO6463364.1"
                     /translation="MTIKEKFSQKYPHASFCTFGDSAALADHLATLIATGVKTASCGS
                     LAGCIEDNAFPMIGEYKIVENSRGEPVCVIRVIGLHLLRFSDVTVELARKEGEGDLSL
                     EYWRNEHRRFFQAEGSYSPEMDVIFEEYALIDVV"
     gene            complement(16938..18320)
                     /gene="yahJ"
                     /locus_tag="ACHKTW_002353"
     CDS             complement(16938..18320)
                     /gene="yahJ"
                     /locus_tag="ACHKTW_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFO6463365.1"
                     /translation="MKESNSRREFLSQSGKMVTAAALFGTSVPLAHAAVAGTLNCEAN
                     NTMKITDPHYYLDNVLLETGFDYENGVAVQTRTARQTVEIQDGKIVALRENKQHPDAT
                     LPHYDAGGKLMLPTTRDMHIHLDKTFYGGPWRSLNRPAGSTIQDMIKLEQKMLPELQP
                     YTQERAEKLIDLLQSKGTTIARSHCNIEPVSGLKNLQNLQAVLARRQAGFECEIVAFP
                     QHGLLLSKSEPLMREAMQAGAHYVGGLDPTCVDGAMEKSLDTMFQIALDYDKGVDIHL
                     HETTPSGVAAINYMVETVEKTPQLKGKLTISHAFALATLNEQQVDALANRMAAQQISI
                     ASTVPIGTLHMPLKQLHDKGVKVMTGTDSVIDHWSPYGLGDMLEKANLYAQLYIRPNE
                     QNLSRSLFLATGDILPLNEKGERVWPKAQDDASFVLVDASCSAEAVARISPRTATFHK
                     GQLVWGSVAG"
     gene            complement(18330..19280)
                     /gene="yahI"
                     /locus_tag="ACHKTW_002354"
     CDS             complement(18330..19280)
                     /gene="yahI"
                     /locus_tag="ACHKTW_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase family protein"
                     /protein_id="HFO6463366.1"
                     /translation="MKELVVVAIGGNSIIKDNASQSIEHQAEAVKAVADTVLEMLASD
                     YDIVLTHGNGPQVGLDLRRAEIAHEREGLPLTPLANCVADTQGGIGYLIQQALNNRLA
                     RHGEKKAVTVVTQVEVDKNDPGFAHPTKPIGAFFSESQRDKLQKANPDWCFVEDAGRG
                     YRRVVASPEPKRIVEAPAIKALIQQGFVVIGAGGGGIPVVRTEAGDYQSVDAVIDKDL
                     STALLAREIHADILVITTGVEKVCIHFGKPQQQALDRVDIATMTRYMQEGHFPPGSML
                     PKIIASLTFLEQGGKEVIITTPECLPAALRGETGTHIIKT"
     gene            complement(19423..20841)
                     /gene="yahG"
                     /locus_tag="ACHKTW_002355"
     CDS             complement(19423..20841)
                     /gene="yahG"
                     /locus_tag="ACHKTW_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HFO6463367.1"
                     /translation="MSQSLFSQPLNVINVGIAMFSDDLKKQHVEVTQLDWTPPGQGNM
                     QVVQALDNIADSPLADKIAAANQQALERIIQSHPVLIGFDQAINVVPGMTPKTILHAG
                     PPITWEKMCGAMKGAVTGALVFEGLAKDLDEAAELAASGEITFSPCHEHDCVGSMAGV
                     TSASMFMHIVKNKTYGNIAYTNMSEQMAKILRMGANDQSVIDRLNWMRDVQGPMLRDA
                     MKIIGEIDLRLMLAQALHMGDECHNRNNAGTTLLIQALTPGIIQAGYSVEKQREVFEF
                     VASSDYFSGPTWMAMCKAAMDAAHGIEYSTVVTTMARNGVEFGLRVSGLPGQWFTGPA
                     QQVIGPMFAGYKPEDSGLDIGDSAITETYGIGGFAMATAPAIVALVGGTVEEAIDFSR
                     QMREITLGENPNVTIPLLGFMGVPSAIDITRVGSSGILPVINTAIAHKDAGVGMIGAG
                     IVHPPFACFEKAILGWCERYGV"
     gene            complement(20841..22388)
                     /gene="fdrA"
                     /locus_tag="ACHKTW_002356"
     CDS             complement(20841..22388)
                     /gene="fdrA"
                     /locus_tag="ACHKTW_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HFO6463368.1"
                     /translation="MSVKIVIKPNTYFDSVSLMSISTRANKLDGVEQAFVAMATEMNK
                     GVLKNLGLLTPELEQAKNGDLMIVINGKSGADNEQLLVEIEELFNTKAQSGSHEARYA
                     TIASAKKHIPESNLAVISVNGLFAAREARQALQNDLNVMLFSDNVSVEDELALKQLAH
                     EKGLLMMGPDCGTAIINGAALCFGNAVRRGNIGIVGASGTGSQELSVRIHEFGGGVSQ
                     LIGTGGRDLSEKIGGLMMLDAIGMLENDPQTEIIALISKPPAPAVARKVLERARACRK
                     PVVVCFLGRVETPVDEQGLQFARGSKEAALKAVMLSGVKQENLDLHTLNQPLIADVRA
                     RLQPQQKYIRGLFCGGTLCDETMFAVMEKHGDVYSNIQPDPEFRLQDINRSIKHTFLD
                     FGDDDFTNGKPHPMIDPTNRISRLIEEARDPEVAVIVMDFVLGFGSHEDPVGSTIEAI
                     KEAKAIAAAEGRELIILAYVLGTDLDTPSLEQQSQMLLDAGVILASSSTNTGLLAREF
                     ICKGEEA"
     gene            complement(22378..23241)
                     /gene="yahE"
                     /locus_tag="ACHKTW_002357"
     CDS             complement(22378..23241)
                     /gene="yahE"
                     /locus_tag="ACHKTW_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HFO6463369.1"
                     /translation="MWALTADADFLAQRGQGQVEQVFARAVNIALPARQQLLTLLCEE
                     YDNAPNSCRLALTHFDDLFRHGDKVQFDDQGITVGQHLHIEMSRCRRWLSPTLQMTAV
                     NFHLIAWLQWHDIIHQHLGENETLFNYRGDNPFYQALNKELHIKRRAVIQAVNEKQNI
                     AAAVASMIGLGIGLTPSADDYLTGLALILFIPGHPSEKYKEEFYLGMQRGRNNTTLLS
                     AITLEAALQQRCRENIHRFIHNIIYDIPGNATQAIEKIKHIGSSSGCDMLYGMADGCA
                     LSQTYGGNYVS"
     gene            complement(23281..23886)
                     /gene="yahD"
                     /locus_tag="ACHKTW_002358"
     CDS             complement(23281..23886)
                     /gene="yahD"
                     /locus_tag="ACHKTW_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HFO6463370.1"
                     /translation="MSIKNLPADYLLAAQQGDIDKVKTCLALGVDINTCDRQGKTAIT
                     LASLYQQYACVQALIDAGADINKQDHTCLNPFLISCLNDDLTLLRIILPAKPDLNCVT
                     RFGGVGLTPACEKGHLSIVKELLAHTEINVNQTNHVGWTPLLEAIVLNDGGIKQQAIV
                     QLLLEHGASPHLTDKYGKTPLELARERGFEEIAQLLIAAGA"
     gene            24144..24641
                     /gene="yahC"
                     /locus_tag="ACHKTW_002359"
     CDS             24144..24641
                     /gene="yahC"
                     /locus_tag="ACHKTW_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HFO6463371.1"
                     /translation="MNGLTATGVTVGICAGLWQLVSSHVGLSQGWELLGTIGFVAFCS
                     FYAAGGGKSGFIRSLAVNYSGMVWAFFAALAAGWLASVSGLSAFWASVITTVPFSAVV
                     VWQGRFWLLSFIPGGFLGMTLFFASGMNWTVTLLGFLAGNCVGIISEYGGQKLSEATT
                     KRDGY"
     gene            24733..25665
                     /gene="yahB"
                     /locus_tag="ACHKTW_002360"
     CDS             24733..25665
                     /gene="yahB"
                     /locus_tag="ACHKTW_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFO6463372.1"
                     /translation="MNSVFTEENLLAFTTAARFGSFSKAAEELGLTTSAISYTIKRME
                     TGLDVVLFTRSTRSIELTESGRYFFRKATDLLNDFYAIKRSIDTISQGIEARVRICIN
                     QLLYTPKHTARLLQVLKKQFPTCQITVTTEVYNGVWDAIINNQANIAIGAPDTLLDGG
                     GIDYTEIGAIRWAFAIAPDHPLAFVPEPIAESQLRLYPNIMVEDTAHTINKKVGWLLH
                     GQESILVPDFNTKCQCQILGEGIGFLPDYMVREAMTQSLLVTRQIHNPRQDSRMLLAT
                     QHSATGQVTQWIKKQFAPNGILTGIYQDLLHREN"
     gene            complement(25707..26795)
                     /gene="pdeL"
                     /locus_tag="ACHKTW_002361"
     CDS             complement(25707..26795)
                     /gene="pdeL"
                     /locus_tag="ACHKTW_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFO6463373.1"
                     /translation="MASHDLSVFLEEFGATVNLTLPGIVSEKERLLLKLLMEGMSVTE
                     ISQYRNRSAKTISHQKKQLYEKLGIQSDITFWRDIFFQYHPQVISGTGNKNNFYIPDN
                     RCHHIVTPEAISLALENHEFKPWIQPVFCAQTGVLTGCEVLVRWEHPQTGIIPPDQFI
                     PLAESSGLIVIMTRQLMKQTADILMPVKHLLPDNFHIGINVSAGCFLAAGFEKECLNL
                     VKKLGNDKIKLVLELTERNPIPVTPEARAIFDSLHQHNITFALDDFGTGYATYRYLQA
                     FPVDFIKIDKSFVQMASVDEISGHIVDNIVELARKPGLSIVAEGVETQEQADLMIGKG
                     VHFLQGYLYSPPVPGNKFISEWVMKAGG"
     gene            complement(26937..30678)
                     /locus_tag="ACHKTW_002362"
                     /pseudo
     CDS             complement(26937..30678)
                     /locus_tag="ACHKTW_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308389.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
BASE COUNT         7435 a         8388 c         7433 g         7781 t
ORIGIN      
        1 gcgcggggtg tcggatgcga cgcgagcgcc ttatccgacc tacggttcag gcctgtaggc
       61 ataataagac gcggtaagcg tcgcatcagg catcggcgca tggtgtcgga tgcgccacgc
      121 gacgcatccg acaatacctt actcttccat cgcctggcgg atctgatcca acgacgctgg
      181 atcatcaatg gtcgtcagat ctccaggatc gcgtccttcg caaatcgcct ggatcgtgcg
      241 gcgcagcatt tttccggatc gcgtttttgg caattgcgag acaaaccaga cgtgcgccgg
      301 gcggccaaag ttgccaatct ggctgtccac cagcgccata atcgccttct cttgcgaatg
      361 cgccacatca cgatcttcca gactgtcgct ctctttcgga atgacaaacg ctaccgccac
      421 ctgccctttc agcgcatctt tcaccccaac caccgccact tcggcaacgc ccggatgact
      481 ggaaatactc tcttcaatct cgcgcgtgcc cagccgatgc ccggcaacgt taatcacatc
      541 gtcagtgcgc ccgagaataa agtgataacc gtcagcatcg cggatgcccc agtcaaaggt
      601 ggcgtacacc ggacgggaaa acagcgacca gtacgtcttc acaaagcggt cgtcatcgcc
      661 ccagatggtc tgaatacacc ccggcggcag cggcccttcc accaccagca tccctttctc
      721 gttgacgcca cacggttcgc cggtgacttc attaagcaac tgcacgttat agccatacat
      781 cggcacgccg gggcttccca gacgcgtcgg cctgtcgtcc agaccgcgag caatcgccat
      841 aatcggccag ccggattcgg tctgccagta gttgtcgatg accggcacat ccagcgtatt
      901 gctcacccaa ctggcggtcg gctcgtccag cggttctcca gccagataga gcacttccag
      961 cgacgagaga tcgtgtttgc gaatttcagc ggtagggaat tttttcagca cgcgaatggc
     1021 ggtcggcgct gagaacatcc ggctaacctg atatttctcg acgattttcc accacacgcc
     1081 gcagtccggc caggtcggta atccttcgta aacgatagtc gccatccccg ccagcagcgg
     1141 cgcgtagacg atatacgaat gccccaccac ccagccgata tccgaagcac aaaagaacac
     1201 accgcccgct ttgccgccaa aaatggtgtc catcgaggtc gccagcgcca ccgcatatcc
     1261 gccgacgtca cgctgcacgc ctttaggttt gccggtcgtg ccggaggtgt agagaatgca
     1321 ggaggtttcg ttggattcca gccacgccac cggtacccgc gcgccgatgt gttgatggcg
     1381 caacgacgcg aaatcgacat cccgcccgct gacgcgcgcc attttcgcca gcccgcgatc
     1441 caccagcaaa acatggcgcg gctggtgctg cgcctgactt atcgcatcgt cgagcaattt
     1501 tttataggga atgattttgc caccgcgcgc tccggcatca gccgagacaa tcagcaccgg
     1561 tttagcatca tcaattcgcg ccgccacgct gtgcgaggca aatccaccaa acaccaccga
     1621 gtgaatagcg ccaatgcgcg cgcaggccag cagggtaata tgcgcttcgg caatcatcgg
     1681 catatacacc agcacccgat cgccacgctg cacgcccagc gagcgcagca ttgacgccac
     1741 cgcgtttact tcgtcatgaa gctggcgaaa ggtaaaggtg cgctcttctt ctgtttccga
     1801 agagacggca atcagcgcca gcgcctctgg ctgtttctcc agccagcgat cgatagcgtt
     1861 gtgacacaag ttggttcggc cttcacaaaa ccaacgggca aacggcggat tgctgtgatc
     1921 gagcgtttgc gtaaagggcg tctgccagtc aatacgccgg gcctgctcgg cccagaactg
     1981 ctccggttcg ttaatcgaac gctgataaaa ttcgctaaaa gacataatgc tctcctgttg
     2041 aacttacgcc ttaccgccgt ttacttaaat gacgtacagg tcgagatact cattgaccgg
     2101 catctgttcc aggcgagctc tgtcgagaga aacctccaga atgcgctgct gctggcgagt
     2161 cgggaactgg cgcgcgagat tgattttgaa tttatccacc agcttcggaa tgccatcctg
     2221 acggcggcga gcatgaccaa tggggtactc caccaccact tcttcaaagc gcgtgccgtc
     2281 ggtgaactca agagtaatgg cattggcgat ggcgcgtttt tccgggtcgt ggtagtcagc
     2341 ggtaaacgtc ggatcttcaa agcaattgat cttctcgcgc agggcgtcaa tgcgtttatc
     2401 ttgcgcaacg ttgtcctcgt aatctgccgc cgttaagcgc ccgaacaaca gcgggatcgc
     2461 caccatgtat tgaatgcagt ggtcgcggtc agccgggtta ttgagcggcc cttttttgtc
     2521 gatgatgcga atacaggctt cgtgggtgcg aatggtcact ttttcgatat ccgccgccgt
     2581 tttgcctgct gcttgcatct gttcatagag cgtcatcgcc gcttcaactg ccgtctggga
     2641 gtggaactcc gccgggaagg agattttgaa cagcacattt tccatgacgt aagaaccgta
     2701 cgggcgctgg aagcggaacg attcaccttt gaaggagacg tcgtagaagc cccacaccgg
     2761 cgcagtcagg gctgacgggt aacccatttc gcccgttttc gccatcagtg ccagacgtac
     2821 cgcgcgggaa gtggcatcgc ccgccgccca ggatttacgc gtgccggtgt tcggcgcatg
     2881 gcgataggtg cgcagcgact gaccgtccac ccacgccagc gaaacggcgt tgagaatttc
     2941 ctcgcgggtc aggccaagca tttcggcgac cacggcggtg gaagccactt tcactaacag
     3001 aacgtggtcg aggccaacgc ggttaaagga gttttccagc gcgatgcagc cctgaatttc
     3061 atgggctttg atcattccgg tcagcacctg tttcatggtc aacggcgctt tgccgctggc
     3121 gaccgcgttg cgcgaaagcc agtctgccgt tgccagaatg ccgccgaggt tgtcggaagg
     3181 atgcccccac tccgccgcca gccaggtatc gttgaaatcg agccaacgga tcatcgcgcc
     3241 gatgttaaat gccgcctgga cggggtcgag ctggaactga gttccgggga cgcgcacgcc
     3301 gttaggtacg acggtgccgg ggacaattgg ccccagcagt tttttacagg ctgggtattc
     3361 gagagcttca agaccgcagc cgagcgtgtc gagcaggcag taatgtgcgg tgtcgtaggc
     3421 tactttggag ctgatttcgt agttcatgac gtaatcgacg atatcaacga tttcacgatc
     3481 aaattccggg cggatgttgt tgatttgagc tgacattggg tacgtttcct tattgttatt
     3541 cgtagaggtt tactggcgct tatccagcgc gacaaactgg cggtcttccg gtccaacata
     3601 attggcggaa ggacggataa ttttgttgtc ctgacgttgt tcgataatgt gcgccgccca
     3661 gccggtgacg cgggcgataa caaacagtgg tgtgaacatc tcggtgggaa cgcccatcat
     3721 gttgtaggaa acagcagaga accagtcgag attggggaac atctttttgc tctcccacat
     3781 caccgtttcc aggcgatcgg cgatgttgta catcttcagc gagccgcctt cctgcgagag
     3841 ctgcttcgcc acacgtttaa tcacctggtg gcgcgggtca gcgatggtgt aaaccggatg
     3901 accaaaacca atgaccactt ctttgttttc cacgcgcttg cggatatctg cttcggcttc
     3961 gtccggcgtt tcgtagcgtt gctggatctc cagcgacact tcattcgccc cgccgtgttt
     4021 tggcccgcgc agtgcgccaa tcgcgccaat aatcgcggaa tacatatcag agccggtgcc
     4081 cgcaatcacc cgactggtaa aggtggaggc gttaaactcg tgttcggcgt acagcaccag
     4141 cgagatatgc atcgcctttt cccagctttg cgatggcttt tcgccgtgca gcagatgcag
     4201 gaagtgaccg ccgatggagt cgtcatcggt ttccggttgg atgcgttcgc cgttgtggct
     4261 gtagtgatac caataaagga gaatcgagct aagcgacgcc agcagtttgt cggcaatatc
     4321 ccgcgcgcca gagacggtat gcccctcttt ttctggcagc gtgcagccga gcgcggaaac
     4381 accggtgcgc ataacatcca tcgggtgtga cgccgccggt aaggcttcca gcacggtacg
     4441 cacgttagcc ggtaaaccac gcagggcttt cagtttcgtt ttgtaagcgg cgagttcgtc
     4501 acgggtcggc agtttgccgt ggatcagcag atgagccact tcttcaaatt cgcaatgttc
     4561 cgccagatca agaatatcgt agccacggta atgcaggtca ttgccacttt tacccacggt
     4621 gcagagcgcc gtattgcccg ccggaacgcc ggaaagtgcc accgattttt tcggtttaat
     4681 gacatgggta ctgttttgca ggatcgttgt gtcgctcata ttgtcctcgt catttgtagg
     4741 gtcgagatat ttttcgttgt gttttatttc acctggctac gggcaaacag gtcgtcgagc
     4801 ttctcttcgt actggtagta gttgatgctt tcgtacagtt cgttgcgggt ctgcatggtg
     4861 tcgatgacgc ttttctgcgt gccttcctgg cgcaggacgt tgtagacatg ttcagcggcg
     4921 cggttcatgg cgcggaacgc tgaaagcggg tacagcgcca ttgcgacatg ggcgctgcgt
     4981 aattcgtcgg tggtaaacag cggcgtggca ccaaattcgg taatgttggc gaggattggc
     5041 acctgaaccg catcggcaaa ctggcggtac atggcgagtt cggtaatcgc ctccgggaac
     5101 aacatctcgg cacccgcttc aacataggcc tgtgcacgct cgatcgccgc atccagcccc
     5161 tctaccgcca gcgcatcggt gcgcgccatg atcacaaaat caggatcggt tttcgcatcc
     5221 accgccgcgc ggatccgatc caccatctcc tctttcgaga cgatcgcttt gttcgggcga
     5281 tgaccgcagc gtttcgcacc aacctgatct tcaatatgca atcctgccgc accggcttta
     5341 atcatcgatt tcaccgtacg cgccacgtta aaggccgaag aaccaaaacc gatatccgca
     5401 tccaccagca gcggcagcga acaaacgtcg gtgatacggc gaatgtcggt cagcacatca
     5461 tcaagggtag aaataccgag atcgggcagc cccagcgaac ctgccgccac gccaccgccg
     5521 gagagataaa ttgcctgata tccggcacgc tgcgccaaca gcgcatgatt agcgttgatg
     5581 gtgccaacaa tctgcaatgg attttcttta gacagtgcag cgcgaaacgc tttacctgga
     5641 gagtgtagag acatagccca tcctttgtta tcaacttgtt acttgcgttg ataaagacaa
     5701 agcaaggggt gtgccacaat taactgaata aaattaaatg tttaatttca atttgttata
     5761 aataaagtca tgaactaatc cgcaaatatg cgtttcagtt aacgtttcag gcaatgtttc
     5821 accgcgtttc attgcaacaa ttatgaaaca agactaaacc caatattcgg tttcttaact
     5881 ttgcggtgcg ctatggcaca tccaccacgg cttaatgacg acaaaccggt tatctggacg
     5941 gtatctgtaa cgcgcctgtt cgagctgttt cgcgatatca gcctcgagtt tgatcacctg
     6001 gcgaacatta cccctatcca gcttggcttt gaaaaagcag tgacctatat ccgcaagaaa
     6061 ctggcaaacg aacgctgcga cgccgtcatc gccgctggct ctaacggcgc gtacctgaaa
     6121 agccgcctgt cagtgccagt tattttgatt aaaccgagcg gctacgatgt gttacaggca
     6181 ctggcaaaag ccggaaagct cacctcttct atcggcgttg tcacttatca ggaaactatt
     6241 ccggcactgg tggcgtttca aaaaaccttt aatttgcgcc tcgatcaacg tagctacatt
     6301 accgaagaag acgcccgcgg gcagattaac gagctcaaag ctaacggcac cgaagcggtg
     6361 gtcggtgcgg ggctgattac cgatctggca gaagaagccg gaatgaccgg aatttttatc
     6421 tattccgccg tcaccgtgcg ccaggcgttc agcgatgcgc tggatatgac gcgcatgtcg
     6481 ttacgccata acactcacga tgccacccgc aacgccctga gaactcgtta cgtgctgggc
     6541 gatatgctcg gtcaatcacc acagatggaa caagtacggc agactatttt gctgtatgcc
     6601 cgctccagtg cggcggtgtt gattgaaggg gaaacgggga cgggcaaaga gctggcggcc
     6661 caggcgattc atcgggaata ttttgcccgc cacgatgcgc gacagggcaa aaagtcgcat
     6721 ccgtttgttg ccgtcaactg cggggcgata gccgaatcgc tgctggaagc ggagctgttt
     6781 ggctatgagg aaggggcgtt taccggctcg cgacgcggag gtcgcgccgg gctgttcgaa
     6841 attgcccacg gcggtacgct gtttctggat gagattggcg aaatgccgct acctttgcag
     6901 actcgcctgt tacgggtgct ggaagaaaaa gaggttactc gcgtcggcgg gcatcagcct
     6961 gttccggtgg atgtgcgggt cattagcgcc actcactgca atctggaaga agatatgcgg
     7021 caagggcagt ttcgccgtga tctgttttat cggctgagta ttttgcgtct gcaattgcca
     7081 ccactgcgcg agcgggtggc ggatattctt ccgctggcgg aaagcttttt gaaagtgtct
     7141 ctggcggcgc tctccgcccc gttttctgcc gcattacgcc agggattaca ggcaagcgaa
     7201 accgtgctgg tgcactacga ctggccaggc aatattcgtg aactgcgcaa tatgatggaa
     7261 cgactggcgc tgtttttaag tgtggaaccg acgccggatt taacgccgca gtttatgcaa
     7321 ctgctactgc cggagctggc gcgcgagtcg gcgaaaattc ccgctccacg cttactgaca
     7381 ccacaacagg cactggagaa atttaaaggc gataaaacag cagcggcgaa ttatttaggc
     7441 ataagccgga cgacgttctg gcggcggctg aaaaactgaa ctgccgaatg cagcgcgaac
     7501 gctttatccg gcataaattt caatagatga tgatcaccga taaatttacg catcggggat
     7561 gtttacatag gtttcagaat tacttcttct tataaatatt tgccgtgccg tggatcttat
     7621 tgtcagtttg accagaggtc agtaccaaca catcagcccc tttttcatcc gcttttttga
     7681 tcaaatcctc tttcgcatct gcggtcgaca tttcattact ggttgaaata tcacctattt
     7741 tttcatactg cgattcaact ttttcaaatt ccgctttggt catcaattca gcggcataga
     7801 cattggtaac ggcaaacgct aacgcaccga ctaacatttt agagattatt ttcatattac
     7861 ggctccagag ttgttatcac actatcaccc acccacatta ttgggttgat taataattag
     7921 ttcagtcagg gcataaacgc cagtaaccgg aaataagaca ctttgtgatc tctttcgcac
     7981 ccgattattt ttggtagaaa tatgatgaag ttacttcact tatttatgga tgaaatcact
     8041 atggatcctt tgcacgccgt ttacctgacc gtaggactgt tcgtgattac tttttttaat
     8101 ccgggagcca atctctttgt ggtagtacaa accagcctgg cttccggtcg acgcgcaggt
     8161 gtgctgaccg ggctgggggt ggcgctgggc gatgcatttt attccgggtt gggtttgttt
     8221 ggtcttgcaa cactaattac gcagtgtgag gagatttttt cgcttatcag aatcgtcggc
     8281 ggcgcttatc tcttatggtt tgcgtggtgc agcatgcgcc gccagtcaac accgcaaatg
     8341 agcacactac aacaaccgat tagcgccccc tggtatgtct tttttcgccg cggattaatt
     8401 accgatctct ctaacccgca aaccgtttta ttttttatta gtattttctc agtaacatta
     8461 aatgccgaaa caccaacatg ggcacgttta atggcctggg cggggattgt gctcgcatca
     8521 attatctggc gagtttttct tagtcaggcg ttttctttgc ccgctgtgcg tcgtgcttat
     8581 gggcgtatgc aacgcgttgc cagtcgggtt attggtgcaa ttattggtgt attcgcgcta
     8641 cgcctgattt acgaaggggt gacgcagcgg tgagcagata atttactctc tacgtaatca
     8701 acctgatttg ccgaatcccg gccatcggcg atgatagccc ccgtaatgcg tggtccttat
     8761 ttccgataag tttacttgag tctttagctt cccatttcag cccactctcc cctgtgtggg
     8821 cttttttcat tggtctgccc cgtcctgaat agccaaacaa tggcaatgtt ccactttgca
     8881 gggatgataa taaggggatc tgattcagaa gagttttgaa aagttggacc gccatcgagg
     8941 cctcgaaccc cgtaccatac aacaatcctg gttgtcgtct gctcttcctg atgagctaat
     9001 ggcggttttt gatatgagcc ttacagcata ttttctgtca ggcttgagtg cgatgcgtaa
     9061 ggtacagaaa agaccccaca caaacatgat ggaattatga tttttttcat atgaatgtca
     9121 acccacacat tcacaaaaca tctgcatgag atcatttttt atacaccgca tagctgcaaa
     9181 accactgctg gtgctttttt aagcgaagcg tcgccgatca caacaaaata tcggcgaaaa
     9241 aggcggtgga atgccatttg gcatacggat atacagatga aatgattacc tgctttgcga
     9301 ttcactctct ggtggaataa caacttgcag gtggaaccct ttctgaacta aagagtgtag
     9361 cggattttaa cgtgcttatt tacaggataa tatctgtcac ctaattcctg cactctattc
     9421 gatatccatt catttaatcg tgcgtcatct ctgtatgttt ttacgtccag aacagagagc
     9481 aataacatca ccagtgattc aggaaaaaca gggtggatat tttttaagtg taataataat
     9541 ccatgcgcaa gcgcctgctt aatgggcgcg tctgtctccg gaatagacga catttcgcga
     9601 agccattttt ccacacgcat acactctttc cctttaggga aaattctcag caaaggcata
     9661 tggtgttcaa gaagaataca tttgcaatct gttcgcactg aatcgataaa ctgcactaat
     9721 ttcttaagcc cggtaaagcg tccagcctgc acatttgctt taatatcaag aaacgcatca
     9781 tcctggtcat atacccaaac tgtccctttt tgattttcaa gtatgcgaaa aacaaactta
     9841 accgggtgat cagggtaatc catccagtca taaaatccag ggaaatcatt aaactgcaca
     9901 tttatggtga gtgcgttatc gtctaaccac tcacagtctc gtacggcagc catgagatga
     9961 atgagtttgt cgtcatcgac taaatcatca cgacaatcat aaggataatc ataatattgt
    10021 ttaatcaaaa ccttgaatat atcgacacag gaaaccaggg agcttttgat atcctctggt
    10081 aactgattgt ttttaaccgt attaagtatg ctttgttgcg tatatggcat caaattattg
    10141 tgcggagctt ttaatgatat catcgcgatc tctcttaaat aaaaataaac gtcgcagata
    10201 ataaagctct cattaaacca ggcactatca gaaatcgctc ataactaacg tgagaagtat
    10261 tgtgtactgg agggagttaa tttaacaatt tgaatttacg ttataggttt tactgctact
    10321 ttgatattat ttcgctctcc ttaactaaaa agttaagtgg agagcgaagt taatatgtta
    10381 aaccacaggg tatttattaa ttttttcagt ctgttagtgt gcgattatcg ataacaaaac
    10441 gatatttcac atcaccacgc agcattcgct cataggcttc attaatttga tcggcccgaa
    10501 tcatctctat atcagccacg atgccatgtt cggcgcaaaa atcgagcatc tcctgagttt
    10561 ctggaatgcc gccaatcata gaaccggcta tcgcacggcg tttcatgatc aggttgaaaa
    10621 cttccggcga tttatgcggt gtcgcaggcg caccaaccag cgtcatggtg ccatcacgct
    10681 tcagcaaggt ggtaaaatcg tcgagattat gtggcgcagc tactgtattc aaaatgaaat
    10741 cgaaactctt cagatgagcc gccatctcat cggcattgcg tgagttaaca acttcatcgg
    10801 cccccagggc ttttgccgct tcgcgttttg cctcagaagt ggtaaatgcc accacatgcg
    10861 cccccatcgc gtgggccagc ttgatcccca tatgtcccag accgccgatg ccgaccacgc
    10921 ccactttttt acccggcccg gcctgccagt gacgtagcgg cgaatacgtg gtgatccctg
    10981 cacacaacaa aggagccacc gccgccagct gctcttgcgg gtgacgaata cgcagaacat
    11041 atcgctcatg aacgacgatc tgttgtgagt agccgcccag agtatggccc ggttcgtccg
    11101 gcgtcggcga gttataggtg ccggtcatgt gatcacagta gttttccagc ccgtcttcac
    11161 actcttcgca atgtttacaa ctgtcgacaa tgcagccgac accgaccaga tcgcccggcg
    11221 catgtttttc tacctgatca ccaacggcta ccacacgccc cacaatttca tgacccggta
    11281 cgcaggggta aaccgtcccc gcccactcgg aacggacctg gtggagatcg gaatggcaga
    11341 cgccacagta agcgatttcg attttgacat cattcggtcc cggttcacgc cgtgtgatat
    11401 ccatcggttc aagtggttgt ttagcggaat atgcaccaac agctttgatc ttcattgtgt
    11461 ttactcctga ttagctatgt gtatttggcg acgcctgata tgtcatcgcg gcaaaacgcg
    11521 tccattgaat atagccaata tttaaaccac ccgatccagc cccaacatgc cgcgaacctg
    11581 aatgaaacag agcagcaacg ttccccataa ggccatcgtc aggtaggggc ttataccgag
    11641 aatattgaag ccgctttcaa taatttgcag aagaaacagc gccagcacca taccaattat
    11701 ccgcccgctg ccgccgtccg gattaacccc accgagtact gccgcgagga ttgacaccag
    11761 caagtacgat tcgccataag aggccttagc cgagttgagt ttcgacatca tcaaaaatgc
    11821 cgctaccgca caaagcaagg cggaaatgac ataaacccaa atcagcaccc gacgagtgtt
    11881 aatgccggag taaagtgttg ctcgttcgtt agacccaatc aggtaaatgg ctttaccgag
    11941 cggcgttttc tccagcaata tccatagccc cagtgccact gcggcaaaca accacatcgg
    12001 tagcggaata ccgaaccatt gcgcatgatt caaccacagc acccatgagg gataattggc
    12061 aatggcgcta ccacctgtca ccagaatatt tatccctttg agcaacgtca tcatcccaag
    12121 cgttgcaaga attggcgaca ctcgaatacc agcaatcaat acgccgttac ataaaccgat
    12181 tatcaccgcc gcgccagcgc ccgccagtaa cgtagcgaca accgtagcaa tacccggtgg
    12241 atactgagtc gccacccacg ccatcaccag tgaacaggcg ttagcagtgg ctataattga
    12301 caagttaatg ccaccgcaaa gcatggtcac cgccatagcc aacgccagaa tgcccagcac
    12361 gggcatttgc gaagcgacgg actggaaatt acttatcgac cagaaaatct ccggcatagt
    12421 aatgctgaac gcggcaacag ttagcaccag caggccgatc aagtaaaact caacgttatt
    12481 acgccacgat tttttcatag cagactccgg ttctgatgct gactccacgc cgtggcacta
    12541 atgctggcaa cgatgatgat gccggtgatc aatgtttgcc agtaagacga gactcccaat
    12601 aaatttagcc cgttttgcat caatgccaac aacaccacgc cgagcaaagt accggttaac
    12661 gtgccgcgcc cgccgagcaa actagtgccg ccaagcacca ccgcagccag tactgtcagc
    12721 tcataaccca gaagagaatc gggggcgaca gtcatcaccg tccacgactg cactacaccc
    12781 gcagcgccag acatcaatcc catatagcca tagacgaaaa gttgcagttt cagcacgcta
    12841 aagccgatgc gactggctga ttcgcggttc ccgccaaggg cataaatttt gcgccctaca
    12901 ctggtgaaat tcatgataaa cgctgttagc agcaccaccg taatcatggc gatcagcggc
    12961 agaccaaggc catagtcata gccatcagca tcggtgtact tgaacaacat aaccccctgc
    13021 tcaaaccacg gcggaaagtc gtacagccac acacctttac tcaaccataa cagcaggcca
    13081 tagaaaatat tcagcgtcga gatagtgatg atgatagaag gcacccgtag gcgattaacc
    13141 agtaaggcgt taattaagcc aagtaaaatg ccgatgcagc ccgccagtgc aaacgcgaca
    13201 gcgaagttgc caccaatttt ttgcagcaac agcaccatgc cgtattgcgc aatgatggtc
    13261 attgctggaa acgaaatatc aattccaccg gaaatcaaca ccacaaacaa accacaggcg
    13321 agaatagtca acatggcata gttattggcg agatcgtaca aattaccgaa tgtcagaaac
    13381 tcgtcgctgc gccaggcaag gccaacaaac agcaccacaa tcagtaatgc cagccagaat
    13441 tcatggcgtt ttttcagttc agccatttac cacctccgca atcctggcct gagaactgct
    13501 gtcaggcagg aagctatggg tgatctgacc tttttgcatt accaaaatcc ggtggctttg
    13561 ataccacgct tcttcaattt catcgcagat catcaatacc gcaatgccat gagccgccag
    13621 atcgctgata atgccgtata ttcctgcttt gttggcgata tcaacaccga ctgtcgggct
    13681 atcgagaatc aacagtcgtg gcccaatcgc cagccatttg gcgatcgata ctcgctgcgc
    13741 gttaccacca gaaagcgtat tcaccggaag atgaggatca gagactttga tggtcaatgc
    13801 cttgattagc ctgtccacca ggtcacgaac tttagtttca ctcagaaagc cgaagcgatc
    13861 ttttaccttg tgaaaaacag tgctaatgat gttgtcttca atagattgcg ccatcaccaa
    13921 accgcgcgac atgcgatctt ccgaaacata accaataccg tggcggatag cgtcctggtt
    13981 actatgcagt gtaaccggct ggccgttgat gaggatctcg ccagcatccg gtcgggtcat
    14041 accaaacaga ctcagacata attccgtgcg ccccgcacca agcaggccaa caatcgatac
    14101 cacttcgccc gcttccaccc gcaagttaat atttagatat tccccatgac gacttaaatt
    14161 acgcacctca agcaccgggg tgctggattt tccctgccac aactctcgca cctgatattc
    14221 gaactcctgg ccggtcatca ggaaaccgag ttgttttgtg gtcatctctg cagccggaaa
    14281 agtcccgacc agctcaccat ctttcagcac tgaaatacgg tcggaaactt ccatcacttc
    14341 ttccagacga tgactgacga agaccacgca gatcccgcgt tcccgcaatt gatgcaccac
    14401 ctgtaataac ccttgcactt cctgatgggt cagcgaagcg gttggttcat ccatcacgat
    14461 caaccgtgcg tcctgcgcca gtgcacggca aattgctacc agctgacagc gggcaattga
    14521 aagctcagaa acgagtgtat ccagtggcaa tgtgacgtta atactggtca ttgccgcctg
    14581 cgccacctca cgcagacgac ggcggttaac aaacagaccg tggtgatagt gattcacggc
    14641 gatattctcc cagacactca ggttaggaaa taaagagagg tcctgataaa tcacctgaat
    14701 cccctgcgcc accgaagcgg cgggtgtcag ctttgaccag tttttcccac caagcgtaat
    14761 ttccgctccc tcatccgggc gataaacacc ggacataatt ttaatcaacg tcgatttacc
    14821 acagccgttt tgcccggcaa ggcagtgcac ttcacccgac ataaagtcga gagaaacatc
    14881 gcgcagcgct cgcgtggcat gaaacgtttt gttgatatga cgaagggaaa ggaaagtttc
    14941 cataacatcc cctggcaata agaatcgggg ctttcgcccc gaagaaatgg attaataaag
    15001 tgagtcgata ttgtctttgt tgaccagtaa taccttatgg aaacggataa ttcgcttatc
    15061 catatctacg tcggccttac ccagattctg catttctaat ccaggtttga tctcttcacc
    15121 cttcagcaac gtgctggcga cggctgcgag tgcgtaacct gcagaggccg gatcgtaagt
    15181 aatcccttcg gtgatatcac cacttttaat cagtgatgcc gcctgtgaag ggatcatcat
    15241 gccatagact gcgactttat ttttcgcccg tttctctttc accgcacgtc ccgcgccaat
    15301 cggaccgttt gaaccaaagg agacaaccgc tttcaagtca ggataggttt tcatcaggtc
    15361 cagtgtggta cgacgtgagt catccacact ctcggcaacc ggcatgcggc gggtaacttc
    15421 atgcatatcc gggtaatgct ctttctggta tttcaccagc aagtcagccc ataagttatg
    15481 ctgcggcacg gtcaaactac ccacgtaaat cacatagccg cccttgcccc ccatgcgttt
    15541 cgccatatgc tcaacatatt cagcggcaaa tttttcgtta tcaatgattt caatatccca
    15601 gttagcactt ggctgaccgg gggattcgtt ggtcagaacc acaattccgg catctcgcgc
    15661 ttttttgaat accggttcca gcacgttggc atcgtttggc acgatagtaa ttgcattaac
    15721 cttacgggcg attaaatcct caataatttt aacttgttgc ggagcatcag tacttgaagg
    15781 ccccacctgt gaggcattaa caccaaaggc tttacccgac tcaaccacac cttcgcccat
    15841 gcgattaaac cacggcatac catcgacttt agaaatattc accacgactt tttccgctgc
    15901 ctggagcggc gcagaaatta gcgcagcgcc taataacagc gaagacacca tattgataac
    15961 aaaacgttta ttcatcattt cagcctcatt gcagggtagt caggaagtgt tcgcccaaat
    16021 tagcgcgctg gagagaataa gaattttcga atatttttat gcagaataaa cagccctatt
    16081 tcaggcagct attaaaaata tggttataaa tatggacaaa acgggcagca acaataagtg
    16141 tgacattcat cacgtaaaaa atagcaaaat aaacaaaatt agcgatattt tatgtgtgtg
    16201 agtttatttc gcgtagataa atagttatct acacaacgtc aataagggca tattcttcaa
    16261 atataacgtc catttctggt gaatagcttc cttctgcctg gaaaaaacga cggtgttcat
    16321 tacgccaata ttcaagactg aggtcacctt caccttcctt acgggcaagc tccacggtga
    16381 catcagaaaa gcgcagtaaa tgcaggccaa tcactctgat aacacataca ggctcaccac
    16441 ggctattttc tacaatctta tactcaccaa tcatcggaaa tgcgttgtct tcaatacatc
    16501 ccgccagcga accacaggaa gctgttttaa caccagtcgc aatcaacgtt gcgaggtgat
    16561 cggccaacgc tgctgaatcg ccaaaagtac aaaaggaagc atggggatat ttttgtgaga
    16621 atttttcttt tatggtcatg gcaatatttt tgcgattacg gaaaaacaaa tcacatctta
    16681 attactgttg gtaaccatca gcaagagtat cattggtttt tcgatactga atctttttta
    16741 tccgaagcag gtcacattta tggcctgata aatacctttg ttaaaactca cttttgtgta
    16801 tttctctcaa ataatcccct ggaaattatg ccgcgagatt ataaagttca ggatcattca
    16861 ttagcaaacc attcccgccc cactccctgg ggcttaaatc tcttacaatt caccgttgca
    16921 ggcgtttcaa atgcgcatca acctgccaca ctcccccaca ccagttgccc tttatggaac
    16981 gttgcggttc tcggcgagat acgcgccacc gcctcggcgg aacaggaggc gtccaccagc
    17041 acaaagctgg catcatcctg cgcttttggc catacacgct cgcctttttc attcagcggc
    17101 aatatatcgc cagtggctag aaacagcgaa cgggagaggt tctgttcgtt aggacgaata
    17161 tagagctgcg cgtagagatt ggctttttcc agcatgtcgc ccagaccata aggcgaccag
    17221 tggtcgataa cgctgtcagt gccagtcatc acttttacgc ctttgtcgtg caactgtttg
    17281 agcggcatat gcagcgtgcc aatcggcacc gtcgaggcga tagaaatttg ctgcgccgcc
    17341 atccgattcg ccagcgcatc gacctgttgt tcgttgagcg tagccagcgc aaaggcgtgg
    17401 ctgatggtca gcttaccttt cagttgcggc gttttctcta ccgtttcaac catataattg
    17461 atggctgcca cgcccgacgg agtggtttcg tgcaggtgaa tatcgacacc tttgtcgtag
    17521 tccagcgcaa tctgaaacat ggtgtcgagg gatttttcca tcgcgccatc gacacaggtc
    17581 gggtccagcc cgccgacgta atgcgcaccc gcctgcatcg cttcgcgcat taagggttcc
    17641 gatttcgaca gcagcaaacc gtgctgcggg aaggcgacga tttcacactc gaagcccgcc
    17701 tgacgccgcg ccagcaccgc ctgcaaattt tgcagatttt ttaggccgga aaccggttcg
    17761 atattgcagt ggctgcgggc aatggtggtg cctttcgact gcaatagatc gatcagtttt
    17821 tccgcccgct cctgcgtata cggttgtaac tccggcagca ttttctgctc aagcttgatc
    17881 atgtcctgga tagtggagcc tgccggacga ttgagcgagc gccacggccc gccgtagaag
    17941 gttttgtcga gatgaatatg catgtcgcga gtggtgggta gcatcaactt accgccagcg
    18001 tcatagtgcg gcagcgtggc gtccggatgc tgcttgttct cgcgcagggc gacaattttg
    18061 ccgtcctgaa tctccacggt ctggcgcgcc gtgcgggtct gcaccgccac accgttttcg
    18121 tagtcaaaac cggtttccag cagcacgtta tcgagatagt aatgcgggtc agtgattttc
    18181 atggtgttgt tcgcttcgca gtttagggtg ccagctaccg ccgcatgggc gagcggcaca
    18241 gaggtaccaa acagcgcggc ggcggtgacc atcttaccgc tctggctcag aaattcacgg
    18301 cggctattgc tttctttcat cctacgtcct tacgttttaa taatatgggt gcccgtttca
    18361 ccgcgcagcg ccgcaggcag gcattccggc gtggtgataa tcacttcttt gccgccctgc
    18421 tccaggaacg tcaggctggc gatgattttt ggcaacatgc tgccgggcgg aaaatgccct
    18481 tcctgcatat agcgagtcat ggtggcaata tccacccgat ccagcgcctg ctgctgcggt
    18541 ttaccaaagt ggatacagac tttttcgacg ccggtggtga tcaccagaat gtcggcgtga
    18601 atttcacggg ccagcagcgc ggtagagaga tctttgtcaa taaccgcgtc cacgctttgg
    18661 taatcccccg cttcagtacg cactaccgga attccaccgc cgcccgcgcc aattacgaca
    18721 aaaccttgtt ggatcagcgc cttaatggcg ggtgcttcga caatacgttt cggttccggc
    18781 gaggcgacca cgcggcgata gccccgtccg gcatcttcaa caaaacacca gtcagggttt
    18841 gccttttgta gtttgtcacg ctggctttca ctgaagaatg caccaatggg cttggtggga
    18901 tgggcaaaac ctggatcgtt tttatccact tccacctgag ttaccacggt gacggctttt
    18961 ttctcgccgt gacgcgccag ccggttattc agcgcctgtt ggatcagata gccgatgccg
    19021 ccctgcgtat ctgccacaca gttcgccagc ggcgttaagg gcagcccttc gcgctcgtgg
    19081 gcaatctccg cacggcgtaa atccagcccg acctgcggcc cgttaccgtg ggtcagcaca
    19141 atgtcgtaat cggaagccag catttccagc accgtatcgg ccacggcttt caccgcctcc
    19201 gcctgatgct caatcgactg gctggcgtta tctttgataa tgctgttgcc accaatggca
    19261 acgaccacaa gctctttcat gttgttatgt ccgctacaga gggtgattag gttatgacca
    19321 acgccagact gcctgatgcg ctacgcttat caggcctaca attctcgggc catccgtagg
    19381 ccggataagg cgttcacgcc gcatccggca tgaacatgta ggtcagacgc cgtaacgttc
    19441 gcaccagcca agaatggctt tctcgaagca ggcaaaaggt ggatgcacaa tgcccgcgcc
    19501 aatcatgccg acgcccgcat ctttatgggc gatggcagtg ttgatcaccg gcagaatgcc
    19561 gctgctaccc acgcgggtga tgtcgattgc cgacggcacg cccataaaac cgagcagcgg
    19621 aatggtgacg ttggggtttt caccgagagt gatttcacgc atctggcgag agaaatcgat
    19681 agcctcttcc accgtgccgc ccaccagcgc aacgattgca ggcgcggtcg ccatagcaaa
    19741 tccgccaata ccgtaggttt cagtgatggc gctgtcgccg atatccagcc ccgaatcttc
    19801 cggcttataa ccggcaaaca tcgggccgat gacctgctgc gccggaccgg taaaccattg
    19861 ccccggtaat ccgctgaccc gcaggccgaa ctcgacgccg ttacgcgcca tggtggtgac
    19921 cacggtgctg tattcgatgc cgtgcgccgc atccatcgcc gctttacaca tcgccatcca
    19981 cgtcgggccg gagaagtagt cgctgctagc gacaaactca aacacttcgc gctgtttctc
    20041 cacggaataa cctgcctgaa taattcccgg cgtcagcgcc tgaatcagca acgtcgtccc
    20101 ggcgttattg cggttatggc actcatcgcc catatgcagt gcctgcgcca gcattaagcg
    20161 cagatcaatc tcgccgataa ttttcatcgc gtcgcgcagc attggcccct gcacatcgcg
    20221 catccagttg aggcggtcga tcacgctctg atcgttggct cccatacgca aaatcttcgc
    20281 catctgctcg ctcatattgg tgtaagcgat gttgccgtag gttttgtttt ttacgatgtg
    20341 cataaacatc gaggcagagg taacacccgc catcgatccc acacagtcgt gttcgtggca
    20401 cggcgagaag gtgatctccc cggaggccgc cagttcagcc gcctcgtcga gatctttcgc
    20461 cagcccttcg aacaccaacg ccccggtgac cgcgcctttc atcgcgccgc acattttttc
    20521 ccaggtgatc ggcggcccgg cgtgaagaat ggttttcggc gtcatgcccg gcaccacgtt
    20581 aatcgcctga tcaaaaccaa tcagcaccgg atgcgactgg ataatgcgct ccagcgcctg
    20641 ctggttagcg gcggcgattt tgtctgccag cggcgaatcg gcaatgttat ccagcgcctg
    20701 caccacctgc atattgccct gccccggcgg cgtccagtcg agttgagtca cttctacatg
    20761 ctgctttttc aggtcatcgc taaacatggc gatgccgacg ttaataacgt tcaatggttg
    20821 gctaaacagt gactggctca tcaggcttcc tcccctttgc agataaattc acgcgccagc
    20881 aatccggtat tggtgctgct gctcgccaga atcactccgg catcaagcag catctggctt
    20941 tgttgttcta acgatggcgt atcaagatcg gtacccagca cataggcgag aatgatcaac
    21001 tcgcgtcctt cggcagcggc gatcgctttc gcttctttga tcgcctcgat ggtggagccg
    21061 accggatctt catgcgatcc aaatccgagc acaaaatcca tcacgatcac cgccacttct
    21121 ggatcgcgcg cctcttcgat caagcgactg atgcggttgg tggggtcaat cattggatgc
    21181 ggcttgccgt tcgtgaagtc gtcatcgcca aagtcgagga aggtgtgttt gatgctgcgg
    21241 ttgatatctt gcaggcggaa ttccggatcg ggctgaatgt tgctgtagac atcgccatgt
    21301 ttttccatca ccgcgaacat ggtttcgtcg cacagcgtgc cgccgcagaa caggccacga
    21361 atgtatttct gctgcggttg cagacgcgca cgcacatcag caatcaacgg ctggttaagc
    21421 gtatgcaggt cgagattttc ctgtttcacg ccggagagca tcaccgcttt tagcgccgcc
    21481 tctttgctgc cgcgggcaaa ctgtagcccc tgctcatcca ccggcgtttc aacgcgaccg
    21541 aggaagcaga ccaccaccgg cttgcggcag gcacgtgcgc gttccagcac tttgcgggcc
    21601 accgcaggcg cgggaggttt tgaaatcagt gcgataattt cagtttgcgg atcgttttcc
    21661 agcatcccga tggcgtcgag catcatcagg ccgccgattt tctcgctcag gtcgcgcccg
    21721 ccggtgccaa tcagttgtga aacaccgcca ccaaattcat gaatgcggac gctcagctcc
    21781 tggctgccgg tgccggatgc gccaacaata ccgatattgc cgcgacgcac ggcgttacca
    21841 aaacagagcg ccgcgccgtt aataatcgcc gtgccgcagt ccggccccat catcagcagc
    21901 cctttttcgt gggccaactg cttgagcgcc agttcatctt caactgagac gttatcggaa
    21961 aacagcatca cgttgagatc gttttgcagc gcctgacgcg cttcgcgagc ggcaaacaga
    22021 ccgttgaccg aaatcaccgc caggttactt tccgggatat gctttttggc gctggcaata
    22081 gtggcgtaac gcgcctcgtg cgagccgctt tgcgctttgg tgttgaacag ttcttcaatc
    22141 tccaccagca actgctcgtt gtccgcaccc gatttaccat tgatgacaat catcaggtcg
    22201 ccgtttttcg cctgttccag ctccggcgtc agcagtccta aatttttcag tacacctttg
    22261 ttcatttcgg tcgccatcgc cacaaatgcc tgctcgacgc cgtcgagttt atttgcacgc
    22321 gtggagatag acatcagcga gacagaatca aaataggtat tcggtttaat gactatttta
    22381 actgacataa ttccctccgt aggtttggct cagcgcacaa ccatcggcca tgccatacag
    22441 catgtcgcag ccggaactgg agccaatatg tttaattttt tctattgcct gagttgcgtt
    22501 cccagggatg tcataaataa tgttgtgaat aaaacgatga atattttccc ggcaacgttg
    22561 ttgtaatgcg gcttccagcg ttatagcact taataatgtg gtgttatttc tgccgcgctg
    22621 cataccgaga taaaattcct ctttgtattt ttccgacgga tgcccgggaa taaataaaat
    22681 aagcgccaga cctgttaaat aatcgtcggc tgatggcgta aggccaatcc ctaacccaat
    22741 catactggcg accgctgcgg cgatattttg tttttcgttt actgcctgaa taactgcccg
    22801 tcgtttaata tgtagttctt tatttaacgc ctgataaaac ggattatcgc cgcgataatt
    22861 aaacagggtt tcattttccc ccaggtgctg atgaataatg tcgtgccact gtagccaggc
    22921 gataaggtga aaattcacag cggtcatttg caaggttggg gacagccaac gccgacaacg
    22981 actcatctct atatgaagat gttgaccaac cgtaatacct tgatcgtcaa actgaacctt
    23041 atcaccatgc cggaacagat catcaaagtg agtgagtgcc aaccgacaac tgtttggcgc
    23101 attgtcgtac tcttcacaaa gcagcgtcag caactgctgg cgagccggga gtgcgatatt
    23161 taccgctctg gcaaagacct gttcaacctg tccttgcccc cgctgcgcca gaaaatccgc
    23221 atccgcagtt agcgcccaca cacaccgtct ttctggtgtg ttgatagcaa gcctcccggt
    23281 ttatgcacct gcggcaatca gtaactgcgc aatctcttca aagccccgtt cccgcgccag
    23341 ttccagtggc gttttgccat atttatcggt cagatgcggg ctggcaccgt gttccagcaa
    23401 taactgcaca atcgcctgct gtttaatacc accatcatta agcacaatcg cttccagtag
    23461 cggcgtccag cctacatggt tggtctggtt aacgttaatc tccgtatgcg ccaaaagctc
    23521 ttttacaata cttaaatggc ctttttcaca ggcaggcgtc aggccgacac cgccaaaacg
    23581 ggttacgcaa ttaagatcgg gtttagccgg taaaataatt cgtagtagcg ttaaatcatc
    23641 gttcagacag ctaattaaaa aaggatttaa acaggtatga tcttgcttat taatatccgc
    23701 tccggcatca attaatgcct gaacgcaagc atattgctga tataaacttg ccagcgtaat
    23761 tgccgttttc ccctgacgat cgcaggtatt aatatcgaca cccagcgcaa ggcaggtttt
    23821 tactttatcg atatcaccct gttgtgcagc caataaatag tcggcaggta gattttttat
    23881 actcattcac attcccctgg tttttgtggc tgttttcgat gaattgagaa tagcaacgat
    23941 aatctgataa aagaacccgc ttaaatttaa ttcgctgtat ttcgcctttt tgttgaatga
    24001 tgttcaacaa tcttttcgca gtgtgcggta gtgcaatatt tgtttctttg acccgatacc
    24061 ctttcactca atcaggtttt atttctctat agtctgcaca tgtaagagta gctttcgtcc
    24121 atgtctaacc agaggggaac aagatgaacg gactcacggc aacgggtgtc accgtcggta
    24181 tttgtgcagg gctttggcag ttagtgtctt cacatgtcgg gctctctcag ggatgggaat
    24241 tattaggaac cattggcttc gtcgcctttt gcagttttta tgccgcaggc ggcggtaaat
    24301 cggggtttat cagaagtctg gcggttaatt attccgggat ggtgtgggca tttttcgccg
    24361 cgctggctgc cggttggctg gcctctgtaa gtggactgtc cgcgttctgg gcgagcgtaa
    24421 ttactaccgt acccttctcg gcggtagtgg tctggcaagg gcgtttctgg ttactgtcct
    24481 ttattcccgg tggctttctc ggcatgacgc tgttttttgc cagcgggatg aactggacgg
    24541 tgacgttact gggttttctg gcgggtaact gcgtggggat aatttcagaa tatggcggac
    24601 aaaaattaag cgaagcgacg actaaacgcg acgggtatta aaagttattc aaaaatgatt
    24661 atctatcttt aatcgatacc tctacagtca tggctggaga aattcatatt agataatggc
    24721 gggaatggtt taatgaactc agtttttacc gaagagaatc tgttggcatt tactaccgct
    24781 gcgcgttttg gcagtttcag taaagccgca gaagagttgg gtttaaccac ttccgccatt
    24841 agctacacca tcaagcgaat ggaaacaggg ctggatgtgg tgctgttcac tcgcagtacc
    24901 cgcagcattg agttaacgga gtccgggcgt tattttttcc gcaaagctac cgacctgctg
    24961 aatgattttt atgccatcaa acgtagcata gatactattt cgcagggcat agaagcgcgt
    25021 gtgcgcattt gtattaatca gctgctttat acgcccaaac acaccgcgcg cttattacag
    25081 gtgctgaaaa agcagtttcc tacctgccag attaccgtca ccaccgaagt gtataacggc
    25141 gtctgggatg cgattattaa taatcaggcc aatatcgcca tcggcgcacc cgatacacta
    25201 ctggacggcg gcggtattga ttacactgaa attggcgcga ttcgctgggc ttttgctatc
    25261 gccccggatc acccgttagc ctttgtgccg gaacccatcg ccgaaagcca actgcgtctc
    25321 taccctaata ttatggtgga ggacaccgcg catacgatta ataaaaaagt gggctggttg
    25381 ctgcacgggc aggagtcaat tctggtgcca gatttcaaca ccaaatgtca gtgccagatt
    25441 ttaggtgaag gaattggttt tttgccggat tacatggtcc gtgaggcgat gacgcaatcc
    25501 ctgctggtta cccgacaaat ccataatccg cgccaggatt cgcgcatgtt actggcgact
    25561 cagcattctg ctacgggtca ggtgacgcag tggattaaaa aacaatttgc gccaaatggc
    25621 atattaacgg ggatctatca ggatttactg catcgggaga attaatcgct gccaacctcc
    25681 cgatgcgctg tgaatagcgg tttacatcaa ccacctgctt tcattaccca ttcagagata
    25741 aatttattac ccggtactgg cggagagtac aaatagccct gcaaaaagtg aacgccttta
    25801 ccgatcatta aatccgcctg ctcctgggtt tctacccctt ccgccacgat actcagacca
    25861 ggcttacgcg ctagttcgac aatattgtcc acaatatgac cggagatttc gtcgacactc
    25921 gccatttgca caaatgactt atcgatctta ataaaatcga ccgggaacgc ctgcaagtaa
    25981 cgataggtcg cataacccgt accaaagtca tccagcgcaa aggtaatgtt gtgctgatga
    26041 aggctgtcaa atatcgctct ggcttctggc gttaccggaa tagggttacg ttccgttagc
    26101 tcgagaacca gcttgatttt atcgttacct aatttcttaa ccaggttcag acactctttt
    26161 tcaaatcccg ctgccaaaaa acaacccgcc gagacgttga tgccaatatg gaaattgtcc
    26221 ggcagcaaat gttttaccgg catcagaata tccgcagtct gtttcatcag ttggcgggtc
    26281 attatgacaa taagaccgga tgactccgcc agaggaataa actgatccgg tgggataatt
    26341 cccgtttgtg gatgttccca gcggacaagt acctcacagc ccgtcagtac cccagtctgc
    26401 gcgcagaaaa ccggttggat ccacggtttg aattcgtggt tttccaacgc cagactgatg
    26461 gcttcaggcg tgacgatatg gtgacaacgg ttatcaggga tataaaaatt atttttattt
    26521 cccgtgccac tgatgacttg cggatgatat tgaaaaaaga tatcccgcca gaaggtaata
    26581 tcactctgga tgcctagttt ttcatacagc tgcttctttt gatgtgaaat tgtctttgca
    26641 ctgcgatttc tgtactgtga gatttctgta accgacattc cctccattag cagcttgagt
    26701 aaaagccgtt ctttttcgga cacgatacca ggcaacgtca aattcaccgt tgccccgaac
    26761 tcttccaaaa aaacactcaa atcatgcgaa gccatcgaac acaccttgat taaatcttgt
    26821 gcgccagcta aatctgcgag tcttcactct gactgataaa gctggctgca agaaaatgac
    26881 acagagagcc gaattatctc ccgttcggct ctctctcctt ttactcatta ttcagatcag
    26941 aattgccact taatgccaac cattgcagag gtgtcattat atcccctatc ggcaacctga
    27001 acgcccacat tcccccagac attcaggttg gcatttagct gtccttccac cccggttttt
    27061 atctcagcaa tatttcgggc tccatcctga gtgacggaca cgccatccat actggtgctg
    27121 aaatccttac tgttatgcag ccagttcact tctacaaacg gctggaactc gcgggattta
    27181 ccgtcatcca ttttatggtg gctcttcagc caggttttta cgccaagtcg ggtttgaaca
    27241 ttgccatcac cgttgctatg aacgagggtt ccgttgcttt cgcggtgctt atcggctttg
    27301 actcccatcc aggtaacctg tgcttgcggc tgaacatacc attcattacg cgttccctgg
    27361 ctgccattaa attcagctaa tttgtgtttg tatccagctt ccagtgaagc ggtaaatcct
    27421 tttgatttat aggattcact ttgtaagtca tcccctttca ctgtgttatc aaaccagctg
    27481 tactgcgccc aactgtcgag atacgcgcca ttacgcgact cgtcatcggc ataccaggtg
    27541 gcatagaggc cggtgctata tccgttcaca ctcgcttttg cacgataacc ggttcgcgag
    27601 gaaatggttt tactgtcgct gttgccatat cccgccatga ccccaacatg ccagctgtcg
    27661 ctgccgtttt ggctccactg cgcgacatcg cctcccagtt gcagaacata gcgattgctt
    27721 tgggttttca gctggccgct gccatcacgc catttattat gaccaccttc atggcgcatc
    27781 cacatagtgg tttgtttctg ctcacccgtc accatgtcgg tatagtacgt attacccaga
    27841 cgctcatgca gacgcgtggt gaacattgta ttcgctgctg caaggttcgc aatgtagcta
    27901 cccgcctccg gtcgcaggtc attatccaca ttcagatccg gacccggcgt aggtgtcggg
    27961 ttagggttcg gctctggatt cgggtttggc tccggattcg gcatcgggtc tggctccggc
    28021 tgcagttcag gagaatcact gccgctggtc agataccagt taccactatt tgccccttgt
    28081 ccacgcgcga gagtgtagtc atacgccccc gcaacgatac gaccagcctg aacaaattcg
    28141 ccctcagact taccgtctac atggataagt tcgataccgt taagggtttt cgcacctgta
    28201 ccgccagcgt tattcaccgt aacggcagtc gtgccgctag tatcgccctc gacaaccaat
    28261 ttatcggtta ctgagtcatc gccattcagt accgtattca ttactagttg accgttatta
    28321 ccgtggtagt tccccttcac cactaacaca ttgccagctt gctgtcctga cattccggtc
    28381 atcagcgtac cgttgttggt cagattgccg cccagggtgt aattcacaga cgcagccgct
    28441 cttttttcag cgctattatt caacgccagg gtaccgttgt tctcaacgtt tgcgctaccc
    28501 aatccctgcg catgacttac caggacatta gccgaattag caatgttcac attcgctgtc
    28561 aggttgctgt tatcgcccgc gatgtccagc gtgccgccct gaatattcag ctctccgctc
    28621 cccgttaacg aaccggttga agtgcccccg ttggtaaccg tcagactgcc gccatttaag
    28681 ctcagcagtg aatcggcgct gccattgagc gtgcccacgg tctggctgtg accattcata
    28741 tccagttcag tctctgccgc cagacgcagt tcatgggtat tacccaacac gttatcgtta
    28801 ttcaacaaaa gcgtcccact gcgcagatcg gtaacaccgg tatagtcgtt gtctttgtta
    28861 gaaagcgata cggtctgacc cttctggctg ctgaatgcca ggtcaccact gccggtaatc
    28921 tttgcgctga gatcggcggc attctcgctt ttaccgttag agctcagcac cagagcatcg
    28981 ctgtcggtag cgtgtaaatc aagctgggtc aggccgtaac cgatatacag accgtcattg
    29041 tttaccccac tcgtcagacg atagtcgtaa gtgccgacgg ccgctttttg cccattctgc
    29101 tgaatatcac gttgggcact gctggagagg atttgcccat ttttatcctg caattgcaga
    29161 ttgcccgcat cgcccagaac ttccgcacct tgcgcatcaa ccaacttaat ggtgctgtta
    29221 ccatcatcga cttcagtgag cgataacgca gagtcaatat cacgagatac tgagcttaca
    29281 acgtcactgt cagaaacctg aatcacaccc tcgccgcgca aatccagcgt tttactaacg
    29341 ttgaccgtcc cttctgtcat ctgtgcacct gctgtcaggg caccaaaatc aacggttccg
    29401 ccggagaatc ccaaagtgct aatggagtga gcgcccttac ccactgttac caggcttccc
    29461 tgacccgcag tgatcccgcc tctctgcaga aactcagcat tcttttccag gttaaatgtc
    29521 gtgttctgga acgccagtga accctggaac cagtctgtat aagcaaactc atcgaaggag
    29581 aattcgttta ttggcaaagg tcatgctatc gtcagccttc aggtgtaatt tcccgcggtt
    29641 gttgatctct gctgtaccta tatttttggt gatccctaaa gcgccatttt gctcaatttt
    29701 gactttacca ctaaaggcag agttatcacc ggttaagtaa gtcgtgccat tgagcgcatt
    29761 gatatagccc aggccctcgc tgccactgac aatatccgcg gaaatagtcg cgtttttatc
    29821 cgtgtggttt aaggtgattt tcttatcgga tgtctttgcc cacacaaact caattttttc
    29881 ggcatcaata atccctgcag ccttcgcagc acttccttcc tgtgcaccta acgctaactc
    29941 agagttggtg cttaaactaa ttttcggtgc ggatatttta cctccatctg atacgactaa
    30001 tgttgtatca gaacgttggt cgtaataacc caggctaata tgttcacgag cattaacttc
    30061 accaccgtta ttaaccgaca ggaactgatt accgccatat ccgccgtaaa cgcccataaa
    30121 gaaggtccct gcatttacct gactaccttc gccgcttaca ttcccctgcg tataagtcag
    30181 acgactggtg cccttaacat gaattttgcc gccattggag acattaaaat agccttgatc
    30241 atgtctcatg cctgagtttg actcatacaa atcatcagca tataactcgc tgccagcatt
    30301 ggagacattc aggaaccccg tacctttcag caaaaagtta agcgggccat tgattgttac
    30361 ctttcctccg gtaatatcca acccaccacc agaagcgaga gtcagacgcg gacgggaagt
    30421 atctgtcgta ttgattacca gattggcatt gtcagtcacc gtaatacggt aatcgatgct
    30481 ctggttctct ttgccaaaat caatagcttt atcctggttt acgatgacat catcggcgaa
    30541 taccagagat gaatataaac ttatcgcgct aattaaaaca gcccgggact tttttccttt
    30601 tactctgctc cccagctccg agcagacgct ccaacagtta cgggacctgt tccactttag
    30661 ccgatatatt ttattcatca caatctccag caatacatcc aaataaaacc aagattgcat
    30721 aaacagcaac gcattaaatg ccattctggt tactgttcat cgcatataaa aattcaaaaa
    30781 ttttgcatag cattggcaac cacatatctg agtatgtagt tcagagggta ataaaatagc
    30841 aagttactac tgaaaactta ttttaagtta acactcttaa ctttatcatt acattcctcc
    30901 cagtctctaa tacctgcgca ttatatgtat ccaggaactc aaatcaactc aaaatataaa
    30961 caaaagcagg aggaatacat ttaatataca gacggtaaaa gtggtaactc ttattaggta
    31021 tccaaatata agaatcg
//
LOCUS       DAZFQD010000052        30803 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.052, whole genome shotgun sequence.
ACCESSION   DAZFQD010000052 DAZFQD010000000
VERSION     DAZFQD010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30803
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.052"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            109..651
                     /gene="zapC"
                     /locus_tag="ACHKTW_002363"
     CDS             109..651
                     /gene="zapC"
                     /locus_tag="ACHKTW_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HFO6463374.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKEQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(648..1757)
                     /gene="ycbX"
                     /locus_tag="ACHKTW_002364"
     CDS             complement(648..1757)
                     /gene="ycbX"
                     /locus_tag="ACHKTW_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HFO6463375.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDRWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRTGICGSCRVQ
                     LLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLAR"
     gene            2001..4109
                     /gene="rlmKL"
                     /locus_tag="ACHKTW_002365"
     CDS             2001..4109
                     /gene="rlmKL"
                     /locus_tag="ACHKTW_002365"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HFO6463376.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQPGIPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWVQHDEDIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERQKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTGRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            4121..6028
                     /gene="uup"
                     /locus_tag="ACHKTW_002366"
     CDS             4121..6028
                     /gene="uup"
                     /locus_tag="ACHKTW_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO6463377.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLLEKEEALRVEELQNAEFDRKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVDGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRALSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKTEEAAAAKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            6158..7411
                     /gene="pqiA"
                     /locus_tag="ACHKTW_002367"
     CDS             6158..7411
                     /gene="pqiA"
                     /locus_tag="ACHKTW_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HFO6463378.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            7416..9056
                     /gene="pqiB"
                     /locus_tag="ACHKTW_002368"
     CDS             7416..9056
                     /gene="pqiB"
                     /locus_tag="ACHKTW_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HFO6463379.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTNNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            9053..9616
                     /gene="pqiC"
                     /locus_tag="ACHKTW_002369"
     CDS             9053..9616
                     /gene="pqiC"
                     /locus_tag="ACHKTW_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HFO6463380.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVTVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLASVWSQEAASIAQEIKRLP"
     gene            9872..10039
                     /gene="rmf"
                     /locus_tag="ACHKTW_002370"
     CDS             9872..10039
                     /gene="rmf"
                     /locus_tag="ACHKTW_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0141014 - ribosome hibernation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HFO6463381.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLNQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(10109..10627)
                     /gene="fabA"
                     /locus_tag="ACHKTW_002371"
     CDS             complement(10109..10627)
                     /gene="fabA"
                     /locus_tag="ACHKTW_002371"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008693 -
                     (3R)-3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HFO6463382.1"
                     /translation="MVDKRESYTKEDLLASGRGELFGAKGPQLPAPNMLMMDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDGRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(10696..12456)
                     /gene="ycbZ"
                     /locus_tag="ACHKTW_002372"
     CDS             complement(10696..12456)
                     /gene="ycbZ"
                     /locus_tag="ACHKTW_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFO6463383.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIANAARTLQSDAGQLVGGHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVTQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRAQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            12642..13094
                     /gene="matP"
                     /locus_tag="ACHKTW_002373"
     CDS             12642..13094
                     /gene="matP"
                     /locus_tag="ACHKTW_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HFO6463384.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            complement(13170..14210)
                     /gene="ompA"
                     /locus_tag="ACHKTW_002374"
     CDS             complement(13170..14210)
                     /gene="ompA"
                     /locus_tag="ACHKTW_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HFO6463385.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     INNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGSVENGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKSNVYGKNHDTGVSPVFAGGVEYAITPEIATRL
                     EYQWTNNIGDAHTIGTRPDNGMLSLGVSYRFGQGEAAPVVAPAPAPAPEVQTKHFTLK
                     SDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLSER
                     RAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEIEV
                     KGIKDVVTQPQA"
     gene            complement(14567..15076)
                     /gene="sulA"
                     /locus_tag="ACHKTW_002375"
     CDS             complement(14567..15076)
                     /gene="sulA"
                     /locus_tag="ACHKTW_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0051782 - negative regulation of cell
                     division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS-induced cell division inhibitor SulA"
                     /protein_id="HFO6463386.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLADDLTEEEHAELVDAANEGNAMGFIMRPVSASSHATRQLSG
                     LKIHSNLYH"
     gene            15295..15924
                     /gene="sxy"
                     /locus_tag="ACHKTW_002376"
     CDS             15295..15924
                     /gene="sxy"
                     /locus_tag="ACHKTW_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HFO6463387.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEVGIKDVRALRILGAKMCWLRLRQQN
                     SLVTEKILFMLEGAIIGIHEAALPVARRQELAEWADSLTPKQEFPAELE"
     gene            complement(15887..18049)
                     /gene="yccS"
                     /locus_tag="ACHKTW_002377"
     CDS             complement(15887..18049)
                     /gene="yccS"
                     /locus_tag="ACHKTW_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YccS family putative transporter"
                     /protein_id="HFO6463388.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGLLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQPYQHDPHFERAFTHIDAALERMRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPHNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAIRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWQFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDEAVCYVDDALHHQPADEERVNEALASLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQITQVPQETPVSA"
     gene            complement(18059..18505)
                     /gene="yccF"
                     /locus_tag="ACHKTW_002378"
     CDS             complement(18059..18505)
                     /gene="yccF"
                     /locus_tag="ACHKTW_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HFO6463389.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            18628..20682
                     /gene="helD"
                     /locus_tag="ACHKTW_002379"
     CDS             18628..20682
                     /gene="helD"
                     /locus_tag="ACHKTW_002379"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HFO6463390.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGDRCDLDTTYRFNSRI
                     GEVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSDGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(20714..21172)
                     /gene="mgsA"
                     /locus_tag="ACHKTW_002380"
     CDS             complement(20714..21172)
                     /gene="mgsA"
                     /locus_tag="ACHKTW_002380"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HFO6463391.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(21268..21930)
                     /gene="yccT"
                     /locus_tag="ACHKTW_002381"
     CDS             complement(21268..21930)
                     /gene="yccT"
                     /locus_tag="ACHKTW_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HFO6463392.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            22103..22516
                     /gene="yccU"
                     /locus_tag="ACHKTW_002382"
     CDS             22103..22516
                     /gene="yccU"
                     /locus_tag="ACHKTW_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFO6463393.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(22561..22878)
                     /gene="hspQ"
                     /locus_tag="ACHKTW_002383"
     CDS             complement(22561..22878)
                     /gene="hspQ"
                     /locus_tag="ACHKTW_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HFO6463394.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(22936..24126)
                     /gene="rlmI"
                     /locus_tag="ACHKTW_002384"
     CDS             complement(22936..24126)
                     /gene="rlmI"
                     /locus_tag="ACHKTW_002384"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HFO6463395.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFSRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPECSIYDRSDVA
                     VRKKEGMELTQGPVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            24221..24499
                     /gene="yccX"
                     /locus_tag="ACHKTW_002385"
     CDS             24221..24499
                     /gene="yccX"
                     /locus_tag="ACHKTW_002385"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HFO6463396.1"
                     /translation="MSKVCIIAWVYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(24496..24825)
                     /gene="tusE"
                     /locus_tag="ACHKTW_002386"
     CDS             complement(24496..24825)
                     /gene="tusE"
                     /locus_tag="ACHKTW_002386"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HFO6463397.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(24916..25575)
                     /gene="yccA"
                     /locus_tag="ACHKTW_002387"
     CDS             complement(24916..25575)
                     /gene="yccA"
                     /locus_tag="ACHKTW_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HFO6463398.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMVANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIIHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(25983..27002)
                     /locus_tag="ACHKTW_002388"
     CDS             complement(25983..27002)
                     /locus_tag="ACHKTW_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO6463399.1"
                     /translation="MGRPRKNKKDNVLPPRVRSNGYSYVWKPEGSTRTIGLGRVRKTS
                     VAKVWQNYELEKAKLHNIMTVAKLWHMFMDSPAFTELAPRTQKDYRQHQKALLMVFGK
                     VLADNVKTEQVRIFMDKRGLESKTQANHELASLSRVYGWGYERGYVKNNPCKGVRKFS
                     LKARTVYITDEQYAAIYAEAIPQLRIAMEISYLCAARLGDVLELKWQDIMDKGIYIEQ
                     NKTGTKQIKEWSPRLRTAIQLARNVSSCTCEYVINTTKGGKVIAKTLNNWWNQAKRSA
                     EQKVGVPFGCNFHDIKAKGISDYEGSSRDKQIFSGHKTENQVLIYDRKTKITPTLDLP
                     LVVSK"
     gene            complement(26980..27222)
                     /locus_tag="ACHKTW_002389"
     CDS             complement(26980..27222)
                     /locus_tag="ACHKTW_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_303789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
                     /protein_id="HFO6463400.1"
                     /translation="MYELTLSPAEIKEITKYERYTKQQHQLRLHGIPFVIGPKNEPIV
                     LRRDIPHGLTTMPKTSELVSAEPDFEALNNGKTKKK"
     gene            complement(27290..29761)
                     /locus_tag="ACHKTW_002390"
     CDS             complement(27290..29761)
                     /locus_tag="ACHKTW_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HFO6463401.1"
                     /translation="MSKVFICAAIPDELATREEGAVAVATAIEAGDERRARAKFHWQF
                     LEHYPAAQDCAYKFIVCEDKPGIPRPALDSWDTEYMQENRWDEESASFVPVEPESDPM
                     NVNFDKLSPEVQNAVLVKFDTCENITVDMVISAQELLQEDMATFGGHIVEAFMKMPEV
                     NSMYPELKLHAIGWVKHKCEPGAKWPEIQAEMRIWKKRREGERKETGKYTSVVDLARS
                     RVNQQNTENAAEKNGAVTVAVRREYKQTWKTLDNELACALWPGDVDAGNIDGSIHRWA
                     KNEVIDNDREDWKRISASMRKQPEALGYDRQTIFGLVRERPIDIHKDPVALNKYITEY
                     LTTKGVFEDEGTNQSTTNTLPSPVPEADAVETAMPDNEKTECKVEVEPSVEREGPFYF
                     LFTDKDGEKYGRANKLSGLDKALAAGATEITKEEYFARKNGTYTGSQQNTDASDTTAQ
                     PDLVKVTADEVNKIMQAASISQPDADKLLAASRGEFVAGISDPNDPKWVKGIETRDSV
                     DQNQQESEQNDQKAEQNSPNALQNEPETKQPEPVVQQEPEKICTACGQTGGGNCPDCG
                     AVMGDATYQETFDEEYQVEVQEDDPEEMEGAEHPHKENAGSAQDHASDSETGETADPL
                     ITVNGHHVITSTSRLWHHLMIDLETMGKNPDAPIISIGAIFFDPQTGDMGPEFSKTID
                     LETAGGVIDRDTIKWWLKQSREAQSAIMTDEIPLDDALLQLREFIDENSGEFFVQVWG
                     NGANFDNTILRRSYERQGIPCPWRYYNDRDVRTIVELGKAIDFDARTVTPFEGERHNA
                     LDDARYQAKYVSAIWQKLIPSQADF"
     gene            complement(29857..30045)
                     /locus_tag="ACHKTW_002391"
     CDS             complement(29857..30045)
                     /locus_tag="ACHKTW_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HFO6463402.1"
                     /translation="MTSAFALVMTVFLITGEPQNVITGIYDSKSSCIQVRDEQKIPGE
                     CLPLKKVSLNLNNEIPAG"
     gene            complement(30042..30230)
                     /gene="dicB"
                     /locus_tag="ACHKTW_002392"
     CDS             complement(30042..30230)
                     /gene="dicB"
                     /locus_tag="ACHKTW_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HFO6463403.1"
                     /translation="METLLPNVNTSEGCFDIGVLLSNREFTEDAIKMRKYEPYLLNDN
                     SILSRIALLELGIIGEQQ"
BASE COUNT         7705 a         7486 c         7948 g         7664 t
ORIGIN      
        1 ttcattgctg ttgcttattt agacattttg tacttttatt gttgaggtta ttaagcgaag
       61 cgacaatgga ttgtgttgct gcggttttat agggtagggg agaggcagat gcgaattaaa
      121 ccagacgata actggcgttg gtattacgat gaagagcacg atcgaatgat gctcgattta
      181 gccaatggta tgctatttcg ctcacgtttt gcgcgcaaga tgttgactcc agatgccttt
      241 tctcccgcag gcttttgcgt tgacgacgcc gcgctctatt tctcttttga agaaaagtgc
      301 cgcgatttta atttatctaa agaacaaaaa gccgaactgg tgctgaatgc actggtagca
      361 attcgctatc tcaaaccgca aatgccgaaa agctggcatt ttgtttccca tggtgaaatg
      421 tgggtgccca tgccgggcga tgcggcctgc gtttggttga gcgatactca cgagcaggtc
      481 aatttgctgg tcgttgaatc tggcgaaaat gccgcactat gcctgctggc acaaccctgc
      541 gttgttattg cgggtcgcgc gatgcagttg ggcgacgcca ttaaaatcat gaacgacagg
      601 ctgaaaccgc aggttaatgt tgacagcttc agcctcgaac aggcagtcta acgcgccaac
      661 ttaagtgcag tcttcggtac acagctacag caaagaatgg tgccatcatc gcccattgct
      721 gattttttca gcggcgtgac ttcgccttct aaaagctgaa cacggcaact tccacaaatg
      781 cccgtgcggc aagaataagg gatacgaatt ccctgatttt ctaattgctc cagcaacacc
      841 tgttggttat ttccacgaaa tgcctgtccc tgccagtcaa tatctacatt tgcgtccggt
      901 tgttgcgtga tgttggcggt atcatcagcg gcagctgcgc cgtaaatttt tgccggagcc
      961 gttgccagaa tttccacctc atcgccaacg cgaatcacgc cgctattacg ggcaattaaa
     1021 ttctgaccaa aatcgacatc gccgttatcc tgggcagtgc ggaaagattg caatgttttt
     1081 aatggttcgc ctgccggatg tttttgccct ttttctgggc tgacggtggt gaaaatacag
     1141 cggctacaag gtttaaccac atcaaacacc acatcaccaa tgcgaatcac tttccagcga
     1201 tcttcttccc aggctgacgc gccggaaacc accagattgg ggcggaattg ctccattttt
     1261 acactggccg gacaacgttg ttggagatca cgtaacgagg cttcgttagc aagaaggtaa
     1321 ggatagccat cagcaaatga cagaggtaca gtgttgtggc gtttcacgcg ccgggtcatt
     1381 tgtggcccca cccagcgtaa ttgcacttcg cgggagaaaa atccacttag ccatttgttg
     1441 atcgcgtctg gcgcaattcg cgcggtaaaa tgtgtgcccc aaacttcggt tggtgcgtct
     1501 tgtgtggcga aatcagcaaa acgaacatat gcgctactgc catctggtgc ggtgagatgc
     1561 aagccatcat gcacaggtga aggagtaaac cgtaccatct ggggaaactg gcgagcggta
     1621 ataaacgtac cgtcaggttc cgtgatcata aagatgcgat cgaaggccag accactgaca
     1681 tcagccagag catgtgtaag accaatgccg cgcatcgatt taacaggatg aataaaaagc
     1741 cgggttaatg tcgccacggt gcggtcctca aatgaaaata agccctcaac tttatgacat
     1801 gacgcgctta ttagctataa tgcgcaacaa ttttcttagc gcctgtccca ttaggctatt
     1861 ttatttgtca gtttggcctt gggttgtgct cgaaatcctc acgtacttgt gtacgctccg
     1921 gtttctccgc gctgtccatg tccagactga ctgcaacaat tacgcctact gcggtaggct
     1981 cttagagtaa aagtgacgat atgaattctc tgtttgccag tacggcccgt gggctggaag
     2041 agctgttaaa aactgaactg gaaaacctgg gggccgttga atgccaggtg gttcagggtg
     2101 gggtccattt caagggcgac acacggcttg tttaccagag cctgatgtgg agccgcctgg
     2161 cctcgcgtat tatgttgccg ctgggcgagt gtaaggttta cagcgattta gacctctatc
     2221 tcggtgttca ggcgatcaac tggacagaga tgtttaatcc tggcgcgacc ttcgctgtcc
     2281 acttcagtgg tttgaatgac accatccgca acagtcagta cggcgcgatg aaagtgaaag
     2341 acgcgatcgt cgatgctttc acgcggaaaa atctgccgcg tccaaatgtt gatcgcgatg
     2401 cgccggatat ccgcgttaac gtctggctgc ataaagaaac cgccagtatc gctcttgatc
     2461 tgagtggtga tggtttacat ctgcgtggct atcgcgatcg tgctggtatt gcgccgatca
     2521 aagaaaccct ggcagccgct attgtgatgc gatccggctg gcagccagga ataccgctgc
     2581 tcgatccgat gtgtggttcc ggtacgttgc tgattgaagc ggcgatgcta gcgaccgatc
     2641 gcgcaccagg cttgcaccgt gggcgttggg gctttagcgg ctgggtgcag catgatgaag
     2701 atatctggca ggaagtgaaa gcggaagcgc aaactcgtgc ccgtaaaggc ctggctgagt
     2761 atagctctca tttttacggt tcggacagcg acgcacgggt gattcaacgt gcgcgcacta
     2821 acgcccgtct tgcggggatt ggtgaactga tcacctttga ggtgaaagat gtcgcgcaac
     2881 tgaccaatcc gctgccgaaa gggccgtacg gtacagtgtt gagcaacccg ccatacgggg
     2941 aacgtctgga cagcgaaccg gcgctgattg cgctgcatag cctgctgggt cggatcatga
     3001 aaaaccagtt tggtggctgg aatctctctt tgtttagtgc ctcgccggat ctgctaagct
     3061 gcctgcagct gcgtgcagac aaacagtaca aggcgaaaaa cggcccgctg gactgcgtac
     3121 agaaaaatta ccacgttgcc gaaagcactc cagacagcaa accggcgatg gtagcggaag
     3181 actacgccaa ccgcctgcgt aagaacctca aaaaattcga gaagtgggct cgtcaggaag
     3241 gaattgaatg ttaccgcctg tatgacgccg atctgccgga atataacgtt gccgttgacc
     3301 gttatgccga ctgggtggtg gtgcaggagt atgcaccgcc aaaaactatt gatgctcaca
     3361 aagcgcgtca gcgtctgttc gatattatcg ctgcaaccat ttcggtactg gggattgcgc
     3421 caaacaaact ggtgctgaaa acccgtgaac gtcagaaggg caaaaatcag taccagaaac
     3481 tgggcgagaa gggcgagttt ctggaagtta ccgaatataa cgctcacttg tgggtgaatc
     3541 tgacggatta cctcgatacc ggtctgttcc tcgatcaccg tatcgcccgt cgtatgctcg
     3601 gccagatgag caaaggcaaa gatttcctca acctgttctc ttataccggc agcgccaccg
     3661 tgcatgcggg attaggcggt gcacgcagca ccaccaccgt ggatatgtcg cgtacttatc
     3721 tggagtgggc agaacgcaac ctgcgtctga atggcctgac cgggcgtgcg catcgcctga
     3781 ttcaggccga ttgcctggcg tggctgcgtg aggcaaatga acagttcgat ctgatcttta
     3841 tcgatccgcc aaccttctct aactcaaaac gaatggaaga tgcgtttgat gttcagcgcg
     3901 atcatctggc gctgatgaaa gatttgaaac gtctgctgcg tgcaggtggg acgatcatgt
     3961 tctcgaacaa taaacgtggc ttccgtatgg atctcgacgg actggcaaaa ctgggactga
     4021 aagcacaaga aattacgcaa aaaacgctct cccaggattt cgcccgtaac cgccagatcc
     4081 acaactgctg gctgattacc gcagcctgaa aggaatagta atgtcattaa tcagtatgca
     4141 tggcgcatgg ctgtcgttca gcgacgcgcc gcttctcgat aacgcagaac tgcatatcga
     4201 agataacgaa cgtgtttgtc tggtaggccg taacggcgcg ggcaaatcga cgttaatgaa
     4261 gatcctcaac cgtgaacagg ggctggatga cgggcgcatt atttacgagc aagatttgat
     4321 cgtggctcgt ctgcaacagg atccgccgcg taacgttgag ggtagcgttt atgatttcgt
     4381 tgccgaaggc attgaagaac aagcggaata tctgaaacgc tatcacgata tttcgcgcct
     4441 ggtgatgaac gacccgagcg agaaaaatct caacgaactg gcgaaggttc aggaacagct
     4501 ggatcaccac aacctgtggc agctggaaaa ccgcatcaac gaagtgctgg cgcaactggg
     4561 gttagatcct aacgttgcac tgtcgtcgct ttccggcggc tggttgcgta aagcggcatt
     4621 aggacgcgcg ctggtgagta atccgcgcgt gctgttgctt gatgaaccga ctaaccacct
     4681 ggatatcgaa accatcgact ggctggaagg gttccttaaa accttcaatg ggacgattat
     4741 tttcatctcc cacgaccgtt cgtttatccg caatatggcg acgcgcattg tcgatctcga
     4801 tcgcggcaag ctggtgacct atccagggaa ttacgaccag tacctgctgg aaaaagaaga
     4861 agccctgcgc gtggaagaac ttcaaaatgc cgagttcgat cgcaaactgg cgcaggaaga
     4921 ggtgtggatc cgccagggga tcaaagcacg ccgtacccgt aatgaaggcc gcgtacgcgc
     4981 cctgaaagca atgcgtcgtg agcgtggcga acgtcgcgaa gtgatgggta ccgcaaagat
     5041 gcaggtggaa gaggccagcc gctccggtaa gatcgttttc gaaatggaag acgtttgcta
     5101 ccaggttgac ggtaagcaac tggtgaaaga tttttcggcc caggttctac gtggcgacaa
     5161 aattgccctg attggtccga atgggtgcgg caaaaccacg ctgctgaaac taatgctcgg
     5221 tcagcttcaa gcggacagcg ggcgtattca cgttggcacc aaactggaag tggcttattt
     5281 cgatcagcac cgcgcggaac tggatcccga taaaacggtg atggataacc ttgccgaagg
     5341 taagcaagag gtgatggtta acggcaagcc acgccacgta ttgggctatt tgcaggactt
     5401 tctgttccat ccgaaacggg cgatgacgcc ggtacgtgcg ctttctggcg gtgagcggaa
     5461 ccgcttgctg ctggcgcgtt tgttcctcaa accaagcaac ttattgattc ttgacgaacc
     5521 gaccaacgat cttgatgtcg aaacgctgga actgctggaa gaactgatcg acagctatca
     5581 gggcacggta ttgctggtta gccacgatcg tcagtttgtc gataacaccg ttacagaatg
     5641 ttggatcttc gaaggcggcg gtaaaattgg tcgttatgtc ggcggttatc atgatgcccg
     5701 tggtcagcaa gagcagtatg tggcgctcaa acagcctgcg gtgaaaaaaa ccgaagaagc
     5761 cgccgcggca aaagcagaaa ctgtaaaacg cagcagtagc aaactaagct ataaattgca
     5821 gcgcgaactg gagcagctac cgcaattgct cgaagatctg gaggcgaagc tggaagccct
     5881 acagacgcag gtggcggatg cttccttctt cagtcagcca catgagcaga cacaaaaagt
     5941 gctcgccgat atggctgctg cagagcagga gctggagcaa gcctttgaac gctgggagta
     6001 tcttgaagcg ttaaaaaatg gtggctgatc gcaaaatagt cgatttaaac agagacagct
     6061 gacaacggta aaattgcctg atgaactggg tttatcaggc atgaaaacca acactttgtt
     6121 agtcatctgt aaaatagcgc atcattaagg agtaccaatg tgcgaacatc atcatgccgc
     6181 gaagcacatc ctgtgctcgc agtgtgacat gctggtggcg ttaccgcgcc ttgagcatgg
     6241 tcagaaagcg gcatgtcccc ggtgtggcac aacgttaacc gtggcgtggg atgcccctcg
     6301 gcagcgtccc accgcctatg cgttggctgc actgttcatg ctgttgctgt ccaacttgtt
     6361 tccttttgtg aatatgaacg ttgcaggagt taccagtgaa attacattac tggaaattcc
     6421 cggcgtgctt ttttctgagg actacgccag cctcggcacc tttttcctgt tgtttgtgca
     6481 actggttccc gcgttttgtc tgataaccat tctgttactg gtgaatcgcg cggaattacc
     6541 ggtccgttta aaagagcaac tggcacgggt gctttttcaa ctcaaaacct ggggaatggc
     6601 ggagattttc ctcgctggtg tgctggtcag tttcgttaaa ctgatggctt acggcagcat
     6661 tggggtaggc agcagctttc tcccctggtg tttattttgt gtcctgcaac tgcgcgcttt
     6721 tcagtgcgtt gatcgtcgct ggttatggga cgacatcgcc ccgatgccag aactgcgcca
     6781 gccgctaaaa ccaggcgtca cggggatacg tcaggggctg cgttcgtgct cctgttgtac
     6841 ggcaatcctt cctgctgatg aacccgtgtg cccgcgttgt agtaccaaag ggtacgttcg
     6901 gcgtagaaac agcctgcagt ggacactcgc gctgcttgta acgtccatca tgctgtatct
     6961 tccggctaat attttgccca tcatggtgac ggatttatta ggctcgaaga tgccgtcgac
     7021 gattctcgct ggggtcattc tgttatggag cgaaggctct tatcccgtcg ctgcggtgat
     7081 ctttctggcc agtattatgg tgccaacgtt aaagatgatc gccatcgcgt ggctgtgttg
     7141 ggatgccaaa gggcatggca agcgcgacag tgaaagaatg catttgattt atgaagttgt
     7201 tgagtttgta ggccgctggt cgatgattga cgttttcgtt atcgcggtgc tctcggcgct
     7261 ggtgcgtatg ggaggtttaa tgagtattta tccggcaatg ggtgcattaa tgtttgcttt
     7321 agtcgtcata atgacaatgt tttctgctat gacgtttgac ccgcgtttgt cgtgggatcg
     7381 tcaacctgaa tcagagcatg aggagtcctg acagtatgga atctaataat ggggaagcca
     7441 aaatccagaa agtgaagaac tggtctcccg tgtggatatt tcctatcgtc acggcgctca
     7501 ttggggcctg ggttcttttt tatcattaca gccatcaggg accggaagtg accctgatca
     7561 ccgcgaatgc ggaaggaatt gaaggtggca aaaccaccat taaaagccgt agcgttgacg
     7621 tcggcgtggt tgaaagcgcc acactggctg atgatttgac gcacgttgaa atcaaagcgc
     7681 ggctgaattc cggtatggaa aaattgctgc ataaagacac cgttttttgg gtggtgaaac
     7741 cgcagattgg tcgcgaaggg attagcggcc tgggaacgct gctgtctgga gtttatatcg
     7801 aactgcagcc aggcgcgaaa ggcagcaaaa tggataaata cgatttgctg gactcgccac
     7861 cgttggcccc gcctgatgcg aaaggtatcc gtgtgattct cgatagcaaa aaagccgggc
     7921 agctctcgcc aggagatccg gtgctgttcc gtggctatcg ggtaggttcg gttgaaacca
     7981 gcaccttcga tacacaaaaa cgcaatatca gctatcaact gttcatcaat gcaccttatg
     8041 accgactggt gaccaacaat gttcgcttct ggaaagatag tggcattgcg gttgatctga
     8101 cgtcagcagg gatgcgtgtg gagatgggct cattgacaac gctgctgagt ggcggtgtca
     8161 gctttgatgt gccggaaggt ctggatttag ggcagccagt ggcaccgaaa acagctttcg
     8221 ttttgtatga tgatcagaag agcattcagg attcgttgta caccgatcac attgattatc
     8281 tgatgttctt taaagattcg gtacgcggtc tgcaaccggg agctccggta gagttccggg
     8341 gtattcgcct gggtaccgta agcaaagtgc cattctttgc gccgaatatg cgtcagacat
     8401 ttaacgatga ttaccgtatt ccggtactga ttcgtatcga gccagagcgg ctgaaaatgc
     8461 agcttggcga aaatgcggat gttgttgagc accttggcga attgttgaaa cgtggtttac
     8521 gcggatcgct gaaaaccgga aacctggtca ctggtgcact gtatgttgat ctcgatttct
     8581 atccaaatac gcctgcaata accggtattc gtgaatttaa tggttatcag attatcccga
     8641 ccgttagcgg cggcctggcg caaatccagc aacgactgat ggaagcgttg gataagatca
     8701 acaaactgcc attgaatccg atgattgaac aggcaaccag tacgctttct gaaagtcagc
     8761 gcacaatgaa aaacctgcaa acgacgctgg atagcatgaa caagatcctc gctagccagt
     8821 cgatgcagca gttgccgacg gatatgcagt caacgttgcg tgaattgaat cgcagcatgc
     8881 agggcttcca gcctggctcc gcagcctaca acaagatggt ggcggatatg cagcgccttg
     8941 atcaggtgtt gcgagaactg caaccggtgc tgaaaacgct caatgagaag agtaacgcgc
     9001 tggtatttga agcgaaggac aaaaaagatc cagagccgaa gagggcgaaa caatgaaaaa
     9061 gtggctagtg acgattgcag cactgtggct ggccggatgc agctccggcg aaattaataa
     9121 aaactattac cagttacctg tggtgcagag cggtacacaa agtaccgcca gccagggcaa
     9181 tcgtctgtta tgggtagagc aggtcactgt tcctgactat ctggcgggga atggtgtggt
     9241 ttatcaaacc agtgatgtga agtatgtgat tgccaacaac aacttgtggg ccagcccgtt
     9301 ggatcaacag ttgcgcaaca ccctggttgc caacctgagt acgcaactgc ccggctgggt
     9361 ggttgcctcc cagcctctgg gaagcgccca ggacacgctc aatgttaccg taacggagtt
     9421 taacggtcgc tatgatggca aggtcattgt cagtggtgag tggctgttga accaccaggg
     9481 acaactgatc aaacgtccgt tccgtctgga aggagtgcaa actcaggatg gttacgatga
     9541 gatggttaaa gtactggcca gtgtctggag tcaggaagcc gcttctattg cgcaagagat
     9601 aaagcgtcta ccttaattat aaagatttgt aaatataacc gtctccggta tgttgcctga
     9661 ggcggttttt ttgtttctaa cgtgcggaaa aatttgttcc tcttcacatt ttttgtacaa
     9721 ccgacatgcc cgtgtagctc acaaatatga cagtggcgtg aattttgcgc attgacggca
     9781 gttatgattc gcggtattgc ttaactgtga ttgcacattt agtaatcact gttttctttt
     9841 ccaccagaaa ccagtatgag ggaaacgagg catgaagaga caaaaacgag atcgcctgga
     9901 acgggcacat caacgtggtt atcaggccgg catcgccgga cgctcaaaag aaatgtgtcc
     9961 ctatcagacg ctgaatcaaa ggtcacaatg gctgggaggc tggcgagaag ccatggcgga
    10021 cagggtagta atggcctgat tctgtctctt taaaaagaaa cctccgcatt gcggaggttt
    10081 cgccttttga tactctgtct gattataatc agaaggcaga cgtatcctgg aacagaccga
    10141 ctttcaggtc gctggcggta tagataagac gaccatcaac cagcacttcg ccatccgcca
    10201 ggcccataat cagacgacgg ttaacaatgc gtttaaagtg aatacggtag gtcacttttt
    10261 tcgctgtcgg cagtacctga ccagtgaatt tcacttcgcc aacgcccagc gcgcggcctt
    10321 taccttcgcc gcccagccag ccgaggtaga accctaccag ctgccacatt gcgtccaggc
    10381 ccaggcatcc cggcataacc ggatcgccaa taaagtggca tccgaagaac cacagatccg
    10441 gattgatatc cagttctgct tcaacatacc ctttgtcgaa gttaccaccc gtttcggtca
    10501 ttttgaccac acggtccatc atcagcatgt tcggtgctgg caattgcggg cctttagcgc
    10561 caaacagttc accgcgacca gaggcaagaa ggtcttcttt tgtataggat tcgcgtttat
    10621 ctaccatgtt ctctgtaagc cttattttat tgaagcacgc aggatagcta acacgtgtac
    10681 gctgaacaag tccgatcagt tcggaataaa ccagttcagc caacgtaatg gccatggaaa
    10741 acggtgacgt ccttcctgtt gcgatgcttg cgcgatacgt tcctggatgg tttgcatcag
    10801 cgtcgtttgg ccttcgccat cccacaccag atttaataat aacggcagtg cgtcagtcac
    10861 atcgtctact gcccagatgg tgaatttgcc ttcttctacc gctttcacca gttcactgtg
    10921 aagacttaaa tggcgaacgt tagctgtggg gataatgaca ccttgtttcc cggttaactc
    10981 acgttgctgg caaatagcaa agaagccttc gattttctca tttaaaccac cgaccggctg
    11041 ggcgcgaccg aactgatcga ctgaacctgt gatagcgata ctctgattca ccggcacatc
    11101 ggcgagggcg cttatcaggg cgcagagttc agccatcgag gcactatctc catcaacttc
    11161 actgtatgac tgctcaaatg tcagcgatgc tgagaagggg atctgttgct caagctgtag
    11221 ttccgacatc aggaacgctt gcatgatcat catccctttc gcatggatat tgccgccaag
    11281 ctccgctttg cgttcgatgt cggtgaattc accatcgcca atatgcacaa cgcagctaat
    11341 gcgagaaggt tcgccaaaag cgcgtggatg acccggaaat tcaatgaccg aaagggcgtt
    11401 aatttgcccg atgcgttcgc cttcggtttc aatcaggatt tgctcctgaa ggatctcatc
    11461 ctgcatacgt tcagcgagga aaccttcgcg ccattcacgc tgctgcagca ttaagtttag
    11521 ctgctcgccg gagaaggtgt cgccatcaca cagggaggcg acctctttac actggcggag
    11581 gatccactgc gggctaagcg gaagtgtttc ttgttcaccg gtgtagcgtg ctgcttcgcg
    11641 gataagtacc ggccaggcat ccgctcccgg tgcaggtaag tgattatgtc tggcggtaaa
    11701 tgtcacccag cgacaccact gggttactga ctctgcatcg acaatctgca gagtatcttc
    11761 aaattcgcta taaatagcct gctctgaaag ctctggctcc atctcctgga aatcagccaa
    11821 tgattcgcgt tcgcctacca gaatgacttt cagcttcaat ggcatcgaag gcacagagac
    11881 ggggagaggg cgcgactcat caaacgcaac ccagtcaaaa cgctcgcggt taacgatatt
    11941 tttcagccgc atccacagca gaggttgcgc cagcagtgta cgcaaagaga tgatgagaat
    12001 accgccattt gcctgatgca ccagaccagg ctgcagggta atgtcgccat taaactggcg
    12061 caggcagcca aagagttgct ccgcttctac ccagtcggca gcgacaactt gcgttaaagt
    12121 cgcaaaatta tcatctgcac tcactgcgtg acgtaagcgg atggagtggc cggaaacctc
    12181 atagtgaccg cccaccagtt ggcctgcatc gctttgtagc gtacgcgcgg cattggcaat
    12241 aagattcaga tactcagact cttccggggc cttcgccagc ataaaagagg aggatgctcg
    12301 cgtatgcagc aactgctcca gtgcgaattg cagtcgcggt tgagtatcac tgaataaagg
    12361 atcgttttcg tcaatcaaat gtggctgagc aaatatttcc tgatagctat cggtatcagg
    12421 aaccaggtca cgccatgcaa gtttcgtaat ggtcaaagtt gatgtttttt agtctgttgt
    12481 caaagccgcg attataccgt aaccggcact acagcacacg tagaaagcac cgacaatact
    12541 cctggcatgg gcgttaaagc tcacaggatg gagattcttt tcttcactgg cctaaaaagc
    12601 tgatattctg taaagagtta cacggtaaca ttgagatcgc tatgaaatat caacaacttg
    12661 aaaatcttga aagcggttgg aaatggaagt atctggtgaa gaagcatcgc gaaggggagt
    12721 taatcacccg ttacatagaa gccagtgccg cccaggaagc cgtcgatgtg ttgctctcgc
    12781 tcgaaaatga acccgtgctg gtaaatggct ggattgacaa gcatatgaat ccggaactgg
    12841 tcaatcgcat gaagcaaacc attcgggcaa gacgaaagcg ccattttaat gcggaacacc
    12901 agcatacgcg caaaaaatcg atcgatctgg aatttattgt ctggcaacgt ctggctggtc
    12961 ttgcgcagcg tcgcggtaaa acgctttctg aaacgattgt tcagctgatt gaagatgcgg
    13021 aaaacaaaga gaaatacgcg aataaaatgt cttctctgaa gcaggatctg caggcattgc
    13081 tgggtaagga ataactgacg aaagtcagtt caatttacta aaggcaaaaa aaaccccgca
    13141 gcagcggggt ttttctacca gacgagaact taagcttgcg gctgagttac aacgtctttg
    13201 atacctttaa cttcgatctc tacgcgacga tccggagcca agcagtcgat cagtgcagca
    13261 cgctgtttca cgttgtcaca ggtgttgcca gtaaccgggt tggattcgcc cataccacgt
    13321 gcggagatct tgtctgccgg gatacctttg gagatcaggt aatcaacaac agactgagca
    13381 cggcgctcgg acagaccctg gttgtaagcg tcagaaccga tgcggtcggt gtaacccaga
    13441 acaactacgg aaccgtcttt cggatccagg ttgctcagct ggctgtacag ctgatccaga
    13501 gcagcctgac cttccggttt cagggttgct ttgttgaagt tgaacagaac gtcagacttc
    13561 agagtgaagt gcttggtctg tacttccggt gccggagctg gagccggagc aactactgga
    13621 gctgcttcgc cctgaccgaa acggtaggaa acacccaggc tcagcatgcc gttgtccgga
    13681 cgagtgccga tggtgtgtgc gtcaccgatg ttgttggtcc actggtattc cagacgggta
    13741 gcgatttcag gagtgatcgc gtactcaaca ccgccagcga agaccggaga aacgccggtg
    13801 tcgtggtttt taccataaac gttggattta gtgtctgcac gccataccat accacccaga
    13861 cgagtgtaga tgtccaggtc gtcagtgatt gggtaaccca gtttagcggt cagttgaacg
    13921 ccctgagctt tgtatgcacc gttttcaacg ctgcctttgt acggcatacg acctaaccag
    13981 tcgtaaccca tttcaaagcc aacatacggg ttaacctggt aaccaccaaa agcaccagcg
    14041 cccagttggt tttcatgggt cgggccattg ttgttgatga aaccagtgtc atggtactgg
    14101 gaccagccca gtttagcacc agtgtaccag gtgttatctt tcggagcggc ctgcgctacg
    14161 gtagcgaaac cagccagtgc cactgcaatc gcgatagctg tctttttcat tttttgcgcc
    14221 tcgttatcat ccaaaatacg ccatgaatat ctccaacgag ataacacggt taaatccttc
    14281 accgggggat ctgctcaata ttaactctac cgatatcttc ggcttatgcc gagcacccct
    14341 ggcgatgtaa agtctacaac gtagttgaaa acttacaagt gtgaactccg tcaggcatat
    14401 gaaaaaaaag tcttgtataa ggtatgttta atcttttttg tcagcgacaa tttacagaag
    14461 aaaatcgcgg aaaccgcttc agacaagcct ccgcaaggaa aattagtcac gactgaaagc
    14521 attggctggg cgacaaaaaa agttccagga ttaatcctaa atttacttaa tgatacaaat
    14581 tagagtgaat ttttagcccg gaaagttgtc tcgtggcgtg agaggatgcg cttaccggac
    14641 gcataataaa ccccatagcg ttaccttcat ttgccgcatc aacaagttca gcatgctctt
    14701 cttcagtcaa atcatctgcc aaccaaccga tcaccacact gtaattgccc gtgcgtaaag
    14761 cgcgaaccat tgactccaca gtgtggcaag gggagagctg gctaatctgc attactttcg
    14821 ttaagggtag cccagatgcc tgaacccatt cccgactcag tttttgttgc ggtgttaacc
    14881 agagttgcca gcgcgattgc tgaccgagtt gctgtaacaa tggcaacagt agaagttgcg
    14941 tcatcatggg ctgatcttcg cgatagacaa cttcactgat aagcccggct gtagtgtttt
    15001 ccgtagagac acgcgcaatt ttacttgctg cggatgagaa cgacgaagaa cgatgtgcat
    15061 agcctgaagt gtacataatc aatccagccc ctgtgagtta ctgtatggat gtacagtaca
    15121 tccagtgaca acaaagatca accctatttt cggaaagagc ctcgcaaatt ttgtcgttgg
    15181 tgacgggaaa acataaatta atcttgcccc ttaagaataa gttgcctatt ttcgtagtta
    15241 acggatccgt taatgtgaat cattctttta tgttatgatt ttaaaaggaa ttttatgaaa
    15301 agcctctcct ataagcggat ctataaatca caagaatacc tggcaacgtt gggcacaatt
    15361 gaataccgat cattgtttgg cagttacagc ctgaccgttg acgacacggt gtttgcgatg
    15421 gtttctgatg gtgagttgta tcttcgggct tgtgagcaaa gtgcacagta ctgtgtaaaa
    15481 catccgcctg tctggctgac atataaaaag tgtggccgat ccgttaccct caattactat
    15541 cgggttgatg aaagtctatg gcgaaatcaa ctgaagctgg tgcgtctgtc gaaatattct
    15601 ctcgatgcag cgctgaaaga gaaaagcacg cgcaataccc gggaaagact gaaagatttg
    15661 cccaatatgt cttttcatct ggaagcgatt ctcggggagg tggggattaa ggatgtacgg
    15721 gcgttacgta tacttggggc aaaaatgtgt tggttgcgac tgcggcagca aaacagtctg
    15781 gtgacagaaa agattctgtt tatgcttgaa ggtgccatta tcggcattca tgaagctgcg
    15841 ctcccggtgg cacgccgcca ggagcttgca gaatgggctg actctcttac gccgaaacag
    15901 gagtttcctg cggaacttga gtaatctggc gttgcagacg accaatctca ggcagtaatg
    15961 caatcaataa tccaacttgt tgtacgacca gaggttcttt gctgtctgcc cgtggttcaa
    16021 gttgctgcat ccgctgtttc aggctagcta atgcctcatt gacgcgttct tcatcagcag
    16081 gttgatgatg taacgcgtca tcaacatagc aaactgcctc atcaagaaac gccagaattt
    16141 caggattagt taactgctcc cggtgagcac cgagggctga gatatagctg gtaaacgtat
    16201 ggttaaggca cagcaaccga aacgcggctt cgcgaatttg cggggtaacg ttcggttcgc
    16261 tggacatatt tgataccacc gacgccagtt cagcatcacg gttgtgtgca tcgcggcggg
    16321 caatacgata cgccagacgg ttatcacgcc cctgatggta ttgctccagt atggcatcga
    16381 gataccggca gttggcctct gtggcgcgtt cgagcattcg cggcagattg cgaaactgcc
    16441 agtcaggcca gatgtagctc actgccgccc acgcaatggc acaaccaatc agcgtatcga
    16501 ttacgcgagg taacgctact tcaaaacctt cacccagcaa gttaaaacac agtagcacca
    16561 aaagtgtgat gaacatcgtt gcatgggcat attgcacgtt acggaaggca aaaaagagca
    16621 cgccggtaat aaccagtagc accagctgcc cttccagtga cggtacaaac cacagcacag
    16681 gaatgccaat ggcgataccg accagcgtac caataatcct taacttcagg cggtggcgcg
    16741 tggcgttata gtttggctgg cagacaaaca aacttgtcag caagatccaa tacccgtgat
    16801 gcattccggt tatttgaatg atggcgtagc cgaagcacaa caccagcgac attcttatcg
    16861 catgacggaa gagggcggat tccggcgtga agtgacggct aagacgcagc cagatatcac
    16921 tcaacccgtg cgggctgtca tcagcgagct cattttcgtc attattatgg ggtagtgcct
    16981 gggcctgttc tgattcaatt gttgccagtt gggcatcaat ggcgcgtaaa ttgttcagca
    17041 aaaatcccag tgttttgagt aaatcggcgg gtgcgccgtt atcgcgcatc cgctccagcg
    17101 cagcatcaat atgcgtaaaa gcgcgctcaa aatgcggatc atgttgataa ggctgacgca
    17161 acaaaataca gcgtgacagt tgctggcacg cctggccctg catcgacatc agccgctgaa
    17221 aacggaacag cacgtcgctg tggcgaaaat gttcacgcaa tgtttgatac tgaatatgag
    17281 aagagctggc acgctcgtga atatcctgtg cgacaaagta ataatgcagc gtgcgacgcg
    17341 ttccccgttg accacgatcg ccacgtaagc gggtcagcag cgagagtttc gtctgattca
    17401 atgtcgccat cagcagaccg ttggcgagag ccaaatcgta cagcggtgcc tggctttcat
    17461 cttcaatatc aggatcaaac atgcgcgact tgagctcaag ataacgcgcc agttgttcat
    17521 agcaacgcgc caggttgtcc tgcagcgggc ggaccgggaa cagcagatga ccaataagtg
    17581 tcaggacgtt gtaccagacg gcaccggcca gcagatacat cggctgctga taccagtgct
    17641 catacagtga tgttcccaac atagtgtaaa tggcgatcag caatgcaccg aaggcaattg
    17701 ttgcatagcg ttgacccaga ccgccgagca aaatgaagcc gctggtagag agcgttaagc
    17761 caatcgcaaa tagccagggc cagggaaaca gcaattctac tgaggccgag gcgataaaaa
    17821 agcagaacag cgtaatgatg aggttacgca aacgtcccgc cagtcggtca tcgagatcgg
    17881 tcagcgccgc tgccaccatc cccagcgtta gcggaatcgt cagttttaca tcacccagcc
    17941 accacggaaa cgctgtggtt ccacaaagcg caataaaaat acgcgcgtaa tacagccagg
    18001 cgctgttcca ggtatagcgt ttgagcaaag gacttagcat aaaggccata ttagtggatt
    18061 attcaaaacg acgacgtgca ttggcttcac gcgcagcttg cgctgtttct accgatacca
    18121 cgcgacgacc aaccggccat agtgcaatag cggcaatttt aaagttcgca atgccgacag
    18181 gaatgccaat gattgaaata cattgtgcga tgcccgttgc aatgtgcatc aggcataacc
    18241 accagccaaa gaaaatcagc cagaaaatat tcaataccgt accgccagta ttcagcagca
    18301 catttttgcc agccgggttc agttcatcga catgaatagc ttcattgcca taaggcacca
    18361 gagacagttt agtgatctcc cagcaggatc gtgtcagcgg taaggtaaaa atcagcacaa
    18421 tactgaccag agtcgccaac agccagccca gagtggtggc aaatccgcca agcacaaagt
    18481 tcagaatgtt caaaacggta cgcataaaac ctcgctttac tgtggttttc agtaatgggc
    18541 ggcaattgta acgttttttt gggctggagc acgttttctc tgacggttac actgataaga
    18601 aataatttcg tgtggatcta cagagtcatg gaactgaaag cgacaacgct tggaaaacgt
    18661 ctggcacagc acccttacga tcgggcggtg atcctcaatg ccgggattaa agtctccggc
    18721 gatcgccacg aataccttat tcctttcaat caattactgg cgattcactg taagcgcggt
    18781 ctggtatggg gcgagctgga atttgtactg ccggacgaaa aagtggtgcg tctgcacggc
    18841 accgaatggg gcgagacgca gcgtttttac catcatcttg atgctcactg gcggcggtgg
    18901 agtggcgaga tgagcgaaat tgcgtctggt gttttacgcc agcaactgga tttgattgcc
    18961 acgcgcactg gagaaaataa atggctgacg cgtgagcaaa cctctggcgt tcagcaacaa
    19021 atccgccagg ctttgtcggc gttgccgttg ccggttaacc gactggaaga attcgataac
    19081 tgccgtgagg cgtggcgtaa atgtcaggcc tggttgaaag atattgaaag cgctcggttg
    19141 cagcataacc aggcgtatac cgaagccatg cttaccgagt atgcggattt tttccgccag
    19201 gtcgagtctt caccgctgaa tccggcgcag gcccgggcag tcgttaatgg cgagcattct
    19261 ctgttagtgc tggcaggtgc aggaagcgga aaaacgtcgg tgctggtggc ccgtgcaggc
    19321 tggttgctgg cgcgtggtga agcgtcccct gagcaaattt tattgctggc gtttggtcgc
    19381 aaagccgctg aagagatgga cgagcgtatt cgcgaacggc tacataccga agacattacc
    19441 gcacgcacgt ttcatgcgct ggcgctgcat attattcagc agggcagcaa aaaagttccg
    19501 atagtcagca aactggaaaa tgataccgct gcccgtcatg aactctttat tgctgagtgg
    19561 cgcaagcaat gcagcgaaaa gaaagcgcag gcgaagggct ggcggcaatg gctgacggaa
    19621 gaaatgcagt ggtcagtgcc agaaggtaac ttctgggatg atgaaaaatt acagcgtcgt
    19681 cttgcctcac gcctcgatcg ttgggtaagt ctgatgcgga tgcacggtgg tgcacaggca
    19741 gaaatgattg ccagtgcacc cgaagagatt cgcgatctgt tcagtaaacg tatcaagttg
    19801 atggccccgt tattaaaagc ctggaaaggt gcgctgaagg cagaaaacgc tgtcgatttt
    19861 tcgggcctta ttcatcaggc gattgtgatt ctggagaaag gccgctttat cagcccgtgg
    19921 aagcatattc tggttgatga atttcaggat atctcgccgc agcgcgcagc gttgttagcg
    19981 gcattacgca agcaaaacag tcagacgacg ttgttcgccg ttggtgatga ctggcaggcg
    20041 atttaccgat tcagcggtgc gcaaatgtcg ctcaccaccg ctttccatga aaactttggt
    20101 gaaggcgatc gctgtgattt agacacgact taccgtttta acagtcgtat cggtgaggtg
    20161 gcaaaccggt ttattcagca gaacccaggc cagctgaaaa agccgctaaa cagcttaacc
    20221 aatggagaca aaaaagccgt cacgttattg gatgagagtc aacttgacgc tttgctggat
    20281 aagctctctg gttatgccaa accggaagag cgcattctga tcctggcgcg ttaccatcac
    20341 atgaggcctg ccagcctgga aaaagcggca acacgctggc cgaagttgca aatcgacttt
    20401 atgaccattc atgccagcaa agggcaacag gcggattacg tcatcatcgt tggcttgcag
    20461 gaggggagtg atggttttcc ggctgcggcg cgggagtcga ttatggaaga ggcgctactg
    20521 ccaccggttg aggatttccc ggacgctgaa gaacggcggt taatgtacgt ggcgctgacc
    20581 cgggcacgcc atcgggtatg ggcactgttt aacaaagaga atccctctcc ctttgtggaa
    20641 atactgaaaa atctggatgt gccggtagcg agaaaaccgt aagaaacagg tggcgtttgc
    20701 cacctgtgca atattacttc agacggtccg cgagataacg ctgataatcg gggatcagaa
    20761 tatcgaccgc gtcgttgaaa tgcggcgact ggattatgaa gtctgccgtt gccacgttgg
    20821 tggcgaccgg aatgttccat accgtcgcca gacgcaacaa ggctttcacg tcaggatcgt
    20881 gcggcacggc atttagtggg tcccagaaga aaatcaatac atcaattttc ccttctgaga
    20941 tcaatgcgcc aacctgctgg tcacccccca ttgggccact caacatcgcg ttgacgttca
    21001 tgccggtcgc gcgggaaatt aagttaccgg tagtgcctgt tgcatacagt acgtgttgtt
    21061 ccagtaacgg ttgatgccgt tccacccagc tcatcagcat ttgtttgcag tgatcgtgtg
    21121 ccaccagcgc aatatgtttc cgcgcaggta aagtgcgagt cgtcagttcc ataatgtaca
    21181 tccgtagtta actttcctac agattactgt aagcacttat cgctgcaaga taaagaccga
    21241 aaaagcctgc gcacaggcac aaaaatctca ggaagatggt tgtttttccg cccattgtag
    21301 gaaagtattt cgcgtttgtg ggtcagccag tttaaaccaa tacttcagcc gttgttctgt
    21361 gagcacctga gactgcggtg gaatagcatc cagctcggaa acacccgaaa gcagcgtact
    21421 gtcatctgcc atcatcgtgg caaattgcgg tagtgaagcg cgtttcgcgg atttgttata
    21481 gcgttcaacc tcgacctcat aatcaatcgt ttttgccgtt gaggtaatgg cgagaatatc
    21541 cagctttacc ggaatcggcg tcgcatcgcc atccaacaat tcaaggcgcg gcgcggcatc
    21601 aaaatggtta gcttcccgct cattctccag gcgaggcagg cgaaaattga cctggttgat
    21661 gagctgggta ttaaaactga ccaccagcgg tggggagata tacagccgtt cttcactatt
    21721 ggaaagatga atcgtcttct caacgcgaaa cactaactga tgcggtccat tatccagttc
    21781 aatgctgtcc gcaccgcgca gcagagagct ggaaaccttc ttgccgtcga gtaccagaag
    21841 atcgacatcg gttgaaagcc gcagcgtggt ggcaaaaacg gataccggca aacataatgc
    21901 aatcaaggtg gtcacgatgc cggttttcat aggtagctcc tgtcaaaaga ccgcccgaat
    21961 aataatgtgt caaaattttt ctttaatcat acttactaac atatagacat atttcccgct
    22021 tttgctctca ttcattcgta ttagctgcat ggttggcatg tcggctttgt cgtacacttt
    22081 gttaaacagt ctggaggaaa taatgaaaga aaccgatatt gcaggcattt taacgtccac
    22141 tcacaccatt gcgctggtgg gggcaagcga caaacccgat cgtcccagct atcgcgtgat
    22201 gaaatatcta ctcgaccagg ggtatcacgt cattccggtt tcgccaaaag ttgccggcaa
    22261 aacgctgctg ggccagcagg ggtatggcac gctggcagac gttccggaaa aggtcgatat
    22321 ggtcgatgtt ttccgtaatt cagaagcggc gtggggcgtg gcacaggaag ctattgccat
    22381 tggcgcgaaa accctctgga tgcaactggg ggtgattaat gagcaagcgg cagtactggc
    22441 acgggatgcc gggttgaatg tggtgatgga tcgctgcccg gctattgaga tccctcgcct
    22501 gggcctggcc aaataaaaaa tccccggaag gcaaaaacct tccggggatt cgttcaggga
    22561 tcagttacgc agacgcgggg cctggagttg tttgcggatg gtctgcgcca gctcatccat
    22621 ggatggctgt tcaggatgtt catcctgcaa ttcgctactt agctgggctt cagccaggta
    22681 ggtatgaacc ggtaggccgt tatcgtcctc catcactacg tgataccacg gagcggcgcg
    22741 aagctcgtca tttaccgcca gctcatcagg cgacggttca gagagcgaat aaaccgggtc
    22801 gatatccacg accactccga gataacctaa cagggaatgg cggacctgct ggccgatacc
    22861 gaatttgctg gcaatcatag tcacctcccg ggaaatcttt acctttgata taagggtaaa
    22921 attccacatt tcaagttaca tgacgcgaca ggcaaaccct ttcagatata gcccttccgg
    22981 ataggtagcg atcaccggat gatcggctgc ctgacggaac tgctctataa attgtacatc
    23041 acggccggca tcaattgcgg catccgcgat gattttctga aataaatcgc tggtcatcag
    23101 accggaacag gagaaagtca ggagaatacc gccttcattc agcaactgaa tcgccagcat
    23161 gttgatatct ttatagccac gacacgcgcc catcaactgg cttttattct caacaaactt
    23221 cggcgggtcc atcacgataa cgtcaaattt ttcaccgcga tcgcgataag tacgcagcaa
    23281 tttaaagaca tcatcacgga caaactcagc cttgctcaga tccagtttgt tcagctcaac
    23341 gttctgccgt gcaatatcca gcgcttcctg ggaggtatca acgctgacaa cctggctgca
    23401 accgcccatc agtgccgata cggcgaaacc accggtatag gagaaacagt tcagcacacg
    23461 tttattttca acgtagcggc gggtagccag gcggctatca cgctggtcca ggtagtagcc
    23521 cgttttgtgt ccgtgctgaa tatccaccag cagtttcatt ccgtgttctt caatcggcag
    23581 cagggcaggt ggcaactcgc cggtgacggg gccctgggtc agctccattc cttctttttt
    23641 acgtaccgcg acgtcgctgc gatcgtaaat cgaacattcc gggtacagcg tttgcagggc
    23701 actaattaat gccgcgcgct gatattctgc cccagcactc agcagttgca gcaccagaaa
    23761 attaccgaaa cgatcgatag taatacccgg caggccatca gattctccgg cgattaaacg
    23821 atagctgtcg aggccatctt tttgcgccag ccagtcacgc catttttgtg cttgttgcaa
    23881 acggcgggaa aaaaaagcaa tgtcgataga ctcagacggg tcaaacgtcc agacgcgcgc
    23941 ccggatttgc gaagctggcg aataagcgcc gcgtgctaac cattttccct gatgatcaac
    24001 aatatcgatg gtttcaccga ggctggcttt accttccatg cgggcaacgg ccccggaaaa
    24061 gacccacgga tggcgacgaa gtaatgattt ttcgcgccct ttggctaaca ctaaacgtac
    24121 actcataatt tacttttctg tcgatgcaaa gaaatgggcg tcattgtccg gagttttcca
    24181 tggatttgca acgcgtccat taaggataag gagagcagca atgtcgaaag tctgcataat
    24241 tgcctgggtt tacgggcgag ttcagggcgt aggatttcgc tacaccacac agtacgaagc
    24301 gaaaagactg ggtttaaccg ggtacgccaa aaatcttgac gatggcagcg tggaagtggt
    24361 tgcttgcggt gaagaagggc aggtggaaaa attaatgcag tggttaaaga gtggcggccc
    24421 gcgttctgcg cgtgtagaac gggtgctcag cgagccgcat catccctcgg gggaattaac
    24481 cgattttcgt atccgttaaa tacattttac cggttttggc aggccagcaa ttttggtggc
    24541 ttgcttagcc ggacctttcg gaaacagtcg gtataaatag cggctgttac ctttttcttc
    24601 gccaaattta ttcgccatcg cttttaccag catacgaatc gccggagaag tattgaattc
    24661 cagatagaaa tcacgcacaa aacgcaccac ttcccagtgt tccggtgaca gcgaaatccc
    24721 ttcgttctct gcaatcacca cagccagcgg ctcactccac tggctgcttt ctttgagata
    24781 gccttcggta tccgtttcta tctctttacc ttcgaagatc agcataatta ttactacctt
    24841 aatcagactg gcggcagtgt aacaaaaaac aaagccccgc ataaagcgag gctatgaaag
    24901 tgttagcggg tgagattaat cgcggctagc gaagcccaga atgctcagca ggctgacgaa
    24961 gatgttgtac agcgaaacat acaggctaac cgtggcacga atatagttcg tttcaccgcc
    25021 atgaatgatg ttgctggttt caaacaaaat agcgccagag gagatcaaaa tgaagaccgc
    25081 gctgatcgcc agatgcagag caggcagctg caggaagata ttcgcaacca taccaatcag
    25141 caccaccaca atacccgcca tcagcatacc accgaggaac gacatatctt tgcgggtggt
    25201 cagcacatat gcagagcagc agaagaacac taacgccgtt ccgcccagtg ccatagcgat
    25261 tacgtcaccc attccggcag acagataggt gttcagaata ggtccgagga tataacccag
    25321 aaaaccggta aaggcgaatg cggagataat cccggtcggc ttattcgccg ttttataggt
    25381 caggaacatc aaaccataca tacccaccag cgtcagaatc agacccggag atggcagcat
    25441 cagcaccgta ctggcagttg cggtaatcgc cgaaaaggcc agcgtcaggc tcagcagaaa
    25501 ataggtatta cgcagcacct tatgggtgct aagcagtgat gtacggtcat gtgaagaact
    25561 aacaatacga tccattgagt cactctctat gacagatgta attaattaag cagcataatg
    25621 ataatgcgta agggcaccca gaagttttac ccatctttac gcatttgatc tggaacaggt
    25681 ttaacagcgg attatcaggt cattaagcaa atataacgcc ctgagaattt cgacaggcaa
    25741 aagaaaaagg ggttagcatt tagctaaccc cctatcttat ttggcggaag cgcagagatt
    25801 cgaactctgg aaccctttcg ggtcgccggt tttcaagacc gaagaaaaac aaatgaaaat
    25861 cagtgtaata cgtgaatctg ctgaaaattt aattaaattt aacaccaaga aaaatcaagg
    25921 tgttaggaag ttgctcatag ctagtatacc aagtattttt gccagaaaat tttctgtatt
    25981 gcctacttgc taaccacgag cggcagatcc agtgttggtg tgatttttgt tttacgatcg
    26041 taaatcaaca cctgattttc tgttttatgc ccgctgaaaa tttgtttgtc gcgactgctg
    26101 ccttcgtaat ctgagatccc cttggctttt atgtcgtgaa aattgcaccc gaacggaacg
    26161 ccaacttttt gctcggctga gcgtttagcc tgattccacc agttgttcag cgttttagct
    26221 atgactttcc cgcctttggt tgtattgatc acatattcgc atgtacagga agatacattt
    26281 cgggctaact ggatcgctgt acgtaatcgc ggtgaccatt ccttgatttg tttggtgccg
    26341 gttttgtttt gctcaatgta gatcccttta tccataatat cctgccattt caactcaagc
    26401 acatcaccga gccttgccgc acagagatag gaaatctcca ttgcaatgcg taactgtgga
    26461 attgcttccg catatatcgc tgcatactgt tcatcggtga tgtaaacagt gcgggcttta
    26521 agagagaatt ttctgactcc tttacatggg ttattcttca cgtatccacg ctcatatccc
    26581 cacccgtata ctcgactcag gcttgccagt tcatgatttg cctgggtctt gctctcaagc
    26641 cctcgtttat ccatgaaaat tcttacctgc tcagttttga cattatcagc aagcactttt
    26701 ccgaatacca tcagcaacgc cttctgatgt tgtcgataat ctttttgggt tcggggggcc
    26761 agttctgtaa atgcagggga gtccataaac atgtgccata atttagctac ggtcattatg
    26821 ttgtggagtt ttgctttttc cagttcataa ttttgccaga ctttagctac gctggttttc
    26881 cgcactcttc ctagccctat agttcttgta cttccttctg gtttccacac gtaactgtaa
    26941 ccattcgatc taacccgcgg tggcagtaca ttatcttttt tattttttct tggtcttccc
    27001 attgttcagt gcctcaaaat cgggttcagc agaaaccagt tcagatgttt ttggcatcgt
    27061 tgtcagtccg tgtggaatat ccctgcgtag aactattggt tcgtttttag gaccgattac
    27121 aaatgggatg ccgtgcagtc ttaactggtg ttgctgtttt gtgtatcgct cgtatttcgt
    27181 gatctcttta atctctgctg gagatagagt taattcgtac atgtggtcac gtgcctttac
    27241 agcatgaccg ccgccactat agctggtggg cggcgatatg ggttgaacat taaaaatcag
    27301 cctgactcgg gatcagtttt tgccagatag ctgaaacgta ttttgcctga taacgggcgt
    27361 catcaagtgc attatggcgc tcaccttcga atggggtaac agttctggca tcgaagtcta
    27421 tggctttccc cagctcaacg attgtgcgta catcgcgatc gttgtagtaa cgccacgggc
    27481 aggggatccc ctgccgttcg tatgaacggc gcaaaatcgt gttgtcgaag ttggctccat
    27541 ttccccagac ctgaacaaaa aattcaccgg agttttcgtc gataaattcc cgcaattgta
    27601 acagtgcatc atctaacggg atttcatcgg tcataatggc agattgcgct tcgcgtgatt
    27661 gcttaagcca ccatttaatg gtgtcccgat caatgacccc gccagcagtt tccagatcga
    27721 tagtcttact aaattccggt cccatatctc cggtttgcgg atcgaaaaat attgcaccta
    27781 ttgagatgat cggggcatca ggattttttc ccatggtttc aaggtcaatc atcagatggt
    27841 gccacaacct gctggtggat gtgataacgt gatgaccgtt caccgtaatt aagggatctg
    27901 ccgtctcgcc agtttcacta tcgctggcgt gatcctgagc gctgccagca ttctccttgt
    27961 gtggatgttc agcgccttcc atttcctccg gatcatcttc ctgaacttca acctgatact
    28021 cttcatcgaa tgtttcctgg tatgttgcgt cgcccatcac cgcgccacaa tcagggcagt
    28081 tgccgccgcc ggtctgaccg caggcggtgc agatcttttc cggttcctgt tgcactactg
    28141 gttcaggttg tttcgtttct ggctcgtttt gtaacgcatt tgggctgttt tgttccgctt
    28201 tctggtcgtt ctgttccgat tcttgctggt tctggtctac agaatcgcgg gtttcaatcc
    28261 cctttaccca tttcggatca ttcgggtcgc taatccctgc aacaaattct ccgcgagagg
    28321 cagcaagcaa cttatcggcg tcaggctggc tgatactggc tgcctgcata attttgttta
    28381 cttcgtcagc ggtaactttt accaggtctg gttgtgcggt cgtgtcagat gcatcagtat
    28441 tttgttgtga acctgtgtat gtgccgtttt tgcgggcaaa atattcttct ttcgtgattt
    28501 cagttgcccc ggcagccagc gccttatcca gaccagaaag tttgtttgcg cgaccgtatt
    28561 tttcgccatc cttgtcggtg aagaggaaat agaacggccc ctcacgctct acagatggtt
    28621 cgacttccac tttgcattcg gttttttcgt tgtccggcat tgccgtttcc actgcatcag
    28681 cttctggtac tggcgacggg agagtattag ttgtgctctg atttgttcct tcatcttcaa
    28741 acacgccctt tgtagtcagg tattcagtga tgtatttgtt cagcgccacg ggatctttgt
    28801 gaatgtcgat cggacgttca cggacaaggc caaaaatagt ctgacggtca tagccaagtg
    28861 cttcaggctg tttgcgcatt gatgccgaga tacgcttcca gtcttcgcgg tcgttgtcga
    28921 taacttcatt ttttgcccag cgatggatgc tgccgtcaat gtttcccgca tccacatcac
    28981 cgggccagag ggcgcaggcc agctcattgt cgagagtttt ccatgtctgt ttgtattcgc
    29041 ggcgaacggc aacagtgaca gctccgtttt tttcagcagc gttttcagtg ttctgttggt
    29101 ttactctgga acgggcgaga tcaacaacag acgtgtattt tccggtttcc ttgcgttcac
    29161 cttcgcggcg ttttttccag atgcgcatct ctgcctgaat ttcaggccat ttagcgccag
    29221 gctcacattt atgcttaacc cacccgatgg catgcagttt aagttcagga tacatggagt
    29281 taacttctgg cattttcata aacgcttcaa cgatatgtcc gccgaatgtt gccatgtctt
    29341 cctgtagtaa ttcctgtgcg ctaatcacca tatcaacggt gatgttttcg catgtgtcga
    29401 atttaaccag gactgcgttc tgtacttcag gggacagctt gtcaaaattg acgttcatcg
    29461 gatcggattc aggttcgacc ggtacaaagg aagctgactc ctcatcccag cgattttcct
    29521 gcatatattc ggtatcccag gaatcgaggg cagggcgggg tatgccgggt ttatcctcgc
    29581 agacaataaa tttataagcg cagtcctgag cagccggata atgttccagg aattgccagt
    29641 gaaattttgc tcgagcacgg cgttcgtcgc cagcttcaat ggctgtggct acagccacag
    29701 cgccttcttc ccttgttgcc agttcgtcag gaatagcggc gcaaataaag actttactca
    29761 ttttgtttta acctcattac agatttcagg gtgaacgaat ccctgccatt gctggcattt
    29821 ttaatccgtt ggtatggcgt taatatggct ggcgggttat ccagccggta tttcgttatt
    29881 caggttcagc gatacttttt ttaacgggag gcattcaccg gggatttttt gttcgtccct
    29941 tacctgaatg caggatgact tactgtcata aattccggta atcacatttt gtggctcacc
    30001 cgttataaga aaaacggtca tcaccagtgc aaatgctgaa gtcactgctg ttctccgata
    30061 ataccaagtt caagaagggc aattctggaa agtatggaat tatcattgag aagataaggt
    30121 tcatattttc tcatcttaat ggcatcttcc gtaaactccc ggttactgag cagaacacca
    30181 atatcaaaac aaccttcaga cgtattaacg tttggtaata acgtttccat tatcgcgtcc
    30241 tcaacaatga attttgtgat gcggtgcctg gtgcctccag gtgacgttaa ccagttaaca
    30301 attaacgccg gaatacagaa ggatgcccgt tacgccccgt aaaagaccac tttacggttt
    30361 taactgttcc gcgtgcgcat agccgcattc accgcatcac aaaattcact ttaaaaaggg
    30421 tggcagagca gtcacggagt aaaactgata ccgccaaacg tcaccagaaa attgataaca
    30481 gagggcgttg cagctgggtt gtcacttaag cgtatggtca acctgacaac ccggtgtcct
    30541 caacggggaa ggaataaccc cgccatactt acctccgcgc catttcgcgg agtgccacaa
    30601 ccggaagcgc acggtcgacg aaaatttaac gacgacctat acagagagac taacttcgcc
    30661 gggcgctttc gtgttatgcc ctgacttttc agggatatat ccttttcagt taactgtcag
    30721 tgccggattc ttatccgtgt ccggcgcacg cactctacct cacctgtgaa taaattaatg
    30781 attaattgat attttgttgt ttg
//
LOCUS       DAZFQD010000053        30784 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.053, whole genome shotgun sequence.
ACCESSION   DAZFQD010000053 DAZFQD010000000
VERSION     DAZFQD010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30784
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.053"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            248..631
                     /gene="secE"
                     /locus_tag="ACHKTW_002393"
     CDS             248..631
                     /gene="secE"
                     /locus_tag="ACHKTW_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFO6463404.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLVAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATVAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            633..1178
                     /gene="nusG"
                     /locus_tag="ACHKTW_002394"
     CDS             633..1178
                     /gene="nusG"
                     /locus_tag="ACHKTW_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFO6463405.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATTLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1337..1765
                     /gene="rplK"
                     /locus_tag="ACHKTW_002395"
     CDS             1337..1765
                     /gene="rplK"
                     /locus_tag="ACHKTW_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFO6463406.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            1769..2473
                     /gene="rplA"
                     /locus_tag="ACHKTW_002396"
     CDS             1769..2473
                     /gene="rplA"
                     /locus_tag="ACHKTW_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFO6463407.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            2886..3383
                     /gene="rplJ"
                     /locus_tag="ACHKTW_002397"
     CDS             2886..3383
                     /gene="rplJ"
                     /locus_tag="ACHKTW_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFO6463408.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            3450..3815
                     /gene="rplL"
                     /locus_tag="ACHKTW_002398"
     CDS             3450..3815
                     /gene="rplL"
                     /locus_tag="ACHKTW_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFO6463409.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            4135..8163
                     /gene="rpoB"
                     /locus_tag="ACHKTW_002399"
     CDS             4135..8163
                     /gene="rpoB"
                     /locus_tag="ACHKTW_002399"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFO6463410.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8240..12463
                     /gene="rpoC"
                     /locus_tag="ACHKTW_002400"
     CDS             8240..12463
                     /gene="rpoC"
                     /locus_tag="ACHKTW_002400"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFO6463411.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            12676..13215
                     /gene="yjaZ"
                     /locus_tag="ACHKTW_002401"
     CDS             12676..13215
                     /gene="yjaZ"
                     /locus_tag="ACHKTW_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase growth adaptation protein"
                     /protein_id="HFO6463412.1"
                     /translation="MKQEVEKWRPFGHPDGDIRDLSFLDAHQAVYVQHHEGKEPLEYR
                     FWVTYSLHCFTKDYEHQTNEEKQSLMYHAPKESRPFCQHRYNLARTHLKRTILALPES
                     NVIHAGYGSYAVIEVDLDGGDKAFYFVAFRAFREKKKLRLHVTSAYPISEKQKGKSVK
                     FFTIAYNLLRNKQLPQSSK"
     gene            complement(13625..14758)
                     /gene="thiH"
                     /locus_tag="ACHKTW_002402"
     CDS             complement(13625..14758)
                     /gene="thiH"
                     /locus_tag="ACHKTW_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HFO6463413.1"
                     /translation="MKTFSDRWRQLDWDDIRLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEQLAQRAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYARHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDNWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAALTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(14755..15525)
                     /gene="thiG"
                     /locus_tag="ACHKTW_002403"
     CDS             complement(14755..15525)
                     /gene="thiG"
                     /locus_tag="ACHKTW_002403"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HFO6463414.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVTLLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAETLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHAAQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEAGLLARQSGPGSRSHFAHATSPLTGFLEASA"
     gene            complement(15527..15727)
                     /gene="thiS"
                     /locus_tag="ACHKTW_002404"
     CDS             complement(15527..15727)
                     /gene="thiS"
                     /locus_tag="ACHKTW_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HFO6463415.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQRQAGAALAINQQIVPRE
                     QWAQHIVQDGDQILLFQVIAGG"
     gene            complement(15711..16466)
                     /gene="thiF"
                     /locus_tag="ACHKTW_002405"
     CDS             complement(15711..16466)
                     /gene="thiF"
                     /locus_tag="ACHKTW_002405"
                     /EC_number="2.7.7.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HFO6463416.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDIDRPKSQVSQQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVARADVVLDCTDNMATRQEINAACVALNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQEPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(16459..17094)
                     /gene="thiE"
                     /locus_tag="ACHKTW_002406"
     CDS             complement(16459..17094)
                     /gene="thiE"
                     /locus_tag="ACHKTW_002406"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HFO6463417.1"
                     /translation="MYQPDFPPVPFRSGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(17094..18989)
                     /gene="thiC"
                     /locus_tag="ACHKTW_002407"
     CDS             complement(17094..18989)
                     /gene="thiC"
                     /locus_tag="ACHKTW_002407"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HFO6463418.1"
                     /translation="MSATKLTRREQRARAQHFIDTLEGTAFPNSKRIYITGTHPGVRV
                     PMREIQVSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARGDTEELTVRSSDYTKARLADDGLDELRFSGVLTPKRAKAGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGARLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAATQTIEMGMADMSENFRARGGEI
                     YLRKEEA"
     regulatory      complement(19070..19170)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(19222..19698)
                     /gene="rsd"
                     /locus_tag="ACHKTW_002408"
     CDS             complement(19222..19698)
                     /gene="rsd"
                     /locus_tag="ACHKTW_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HFO6463419.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            19793..20566
                     /gene="nudC"
                     /locus_tag="ACHKTW_002409"
     CDS             19793..20566
                     /gene="nudC"
                     /locus_tag="ACHKTW_002409"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HFO6463420.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLVQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSILLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            20606..21670
                     /gene="hemE"
                     /locus_tag="ACHKTW_002410"
     CDS             20606..21670
                     /gene="hemE"
                     /locus_tag="ACHKTW_002410"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HFO6463421.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRRELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLVRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            21680..22351
                     /gene="nfi"
                     /locus_tag="ACHKTW_002411"
     CDS             21680..22351
                     /gene="nfi"
                     /locus_tag="ACHKTW_002411"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312948.1"
                     /note="cleaves DNA at apurinic or apyrimidinic sites;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HFO6463422.1"
                     /translation="MDLASLRAQQIELASSVIREDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLLVDVPTIGVAKKRLCGKFEPLSSEPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            22394..22984
                     /gene="yjaG"
                     /locus_tag="ACHKTW_002412"
     CDS             22394..22984
                     /gene="yjaG"
                     /locus_tag="ACHKTW_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HFO6463423.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMSDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLREAGESNIGIIFQQ"
     gene            23171..23443
                     /gene="hupA"
                     /locus_tag="ACHKTW_002413"
     CDS             23171..23443
                     /gene="hupA"
                     /locus_tag="ACHKTW_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-alpha"
                     /protein_id="HFO6463424.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            23456..24151
                     /gene="yjaH"
                     /locus_tag="ACHKTW_002414"
     CDS             23456..24151
                     /gene="yjaH"
                     /locus_tag="ACHKTW_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HFO6463425.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALFLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRAAQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(24153..24578)
                     /gene="zraP"
                     /locus_tag="ACHKTW_002415"
     CDS             complement(24153..24578)
                     /gene="zraP"
                     /locus_tag="ACHKTW_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HFO6463426.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYTQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            complement(24581..24709)
                     /locus_tag="ACHKTW_002416"
     CDS             complement(24581..24709)
                     /locus_tag="ACHKTW_002416"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6463427.1"
                     /translation="MTRYPQQASHFYSFIMPDYPSYQRFIVGTEDAIPEVRQPLED"
     gene            24816..26192
                     /gene="zraS"
                     /locus_tag="ACHKTW_002417"
     CDS             24816..26192
                     /gene="zraS"
                     /locus_tag="ACHKTW_002417"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HFO6463428.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMHR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVGQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTVSESGAGVKISVTDSGKG
                     IAADQLEAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGSTFTLWLPVN
                     ITRKDPQG"
     gene            26189..27514
                     /gene="zraR"
                     /locus_tag="ACHKTW_002418"
     CDS             26189..27514
                     /gene="zraR"
                     /locus_tag="ACHKTW_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HFO6463429.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VRERVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGALD
                     YLIKPLDFDTLQATLEKALAHTHSIDAETPAVTASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADKRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLASHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERSVVLLTGEYISERELPLAIASTPIP
                     LGQSQDIQLLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(27511..28800)
                     /gene="purD"
                     /locus_tag="ACHKTW_002419"
     CDS             complement(27511..28800)
                     /gene="purD"
                     /locus_tag="ACHKTW_002419"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFO6463430.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGLKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALAYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDTGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLGV
                     VMAAGGYPGDYRTGDVIHGLPLEEVAGGKVFHAGTKLADNEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTNIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(28812..30401)
                     /gene="purH"
                     /locus_tag="ACHKTW_002420"
     CDS             complement(28812..30401)
                     /gene="purH"
                     /locus_tag="ACHKTW_002420"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFO6463431.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTQRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7250 a         7982 c         8425 g         7127 t
ORIGIN      
        1 gcaaaagttc tgagctaatt gccgataaca tttgacgcaa tgcgcactaa aagggcatca
       61 tttgatgccc tttttgcacg ctttcgtacc agaacctggc tcatcagtga ttttctttgt
      121 cataatcatt gctgagacag gctctgttga gggcgtataa tccgaaaagc taatacgcgt
      181 ttcgatttgg tttgcctcgc gatcgcgggg tgaaaatgtt tgtagaaaac ttctgacagg
      241 ttggtttatg agtgcgaata ccgaagctca aggaagcggg cgcggcctgg aagcgatgaa
      301 gtgggtcgtt gtggtggcat tgctcctggt ggcgattgtc ggcaactacc tttatcgcga
      361 cattatgctg ccgctgcgtg cgctggccgt agtaattctg attgctgcag cgggtggtgt
      421 cgcgctgtta acgacaaaag gtaaagctac cgttgctttt gcccgtgaag cgcgtaccga
      481 agtccgtaag gtcatttggc cgactcgcca ggaaacattg cacaccacgc tgattgtggc
      541 tgcggttacc gcagtaatgt cactgatcct gtggggactg gatggtattc tggttcgcct
      601 ggtatccttt atcactggcc tgaggttctg agatgtctga agctcctaaa aagcgctggt
      661 acgtcgttca ggcgttttcc ggttttgaag gccgcgtagc aacgacgctg cgtgagcata
      721 tcaaattaca caacatggaa gatttgtttg gtgaagtcat ggtaccaacc gaagaagtgg
      781 ttgaaatccg tggcggtcag cgtcgcaaaa gcgaacgtaa attcttccct ggctacgtcc
      841 tcgttcagat ggtgatgaac gacgcgagct ggcacctggt gcgcagcgta ccgcgtgtga
      901 tgggcttcat cggcggtact tccgatcgtc ctgcgccaat cagcgataaa gaagtcgatg
      961 cgattatgaa ccgcctgcag caggttggtg ataagccgcg tccgaaaacg ctgtttgaac
     1021 cgggtgaaat ggtccgtgtt aatgatggtc cgttcgctga cttcaacggt gttgttgaag
     1081 aagtggatta cgagaaatct cgtctgaaag tgtctgtttc tatcttcggt cgtgcgaccc
     1141 cggtagagct ggacttcagc caggttgaaa aagcctaacc cagcgatcaa aaaagcggcg
     1201 atttaatcgt tgcacaaggc gtgagattgg aatacaattt cgcgcctttt gtttttatgg
     1261 gccttgcccg taaaacgatt ttttatatca cggggagcct ctcagaggcg ttattaccca
     1321 acttgaggaa tttataatgg ctaagaaagt acaagcctat gtcaagctgc aggttgcagc
     1381 tggtatggct aacccgagtc cgccagtagg tccggctctg ggtcagcagg gcgtaaacat
     1441 catggaattc tgcaaagcgt tcaacgcaaa aactgattcc atcgaaaaag gtctgccgat
     1501 tccggtagta atcaccgttt acgctgaccg ttctttcact ttcgttacca agactccgcc
     1561 ggcagcagtt ctgctgaaaa aagcggctgg tatcaagtct ggttccggta agccgaacaa
     1621 agacaaagtg ggtaaaattt cccgcgctca gctgcaggaa atcgcgcaga ccaaagctgc
     1681 cgacatgact ggtgccgaca ttgaagcgat gactcgctcc atcgaaggta ctgcacgttc
     1741 catgggcctg gtagtggagg actaagaaat ggctaaactg accaagcgca tgcgtgttat
     1801 ccgcgagaaa gttgatgcaa ccaaacagta cgacatcaac gaagctatcg cactgctgaa
     1861 agagctggcg actgctaaat tcgtagaaag cgtggacgta gctgttaacc tcggcatcga
     1921 cgctcgtaaa tctgaccaga acgtacgtgg tgcaactgta ctgccgcacg gtactggccg
     1981 ttccgttcgc gtagccgtat ttacccaagg tgcaaacgct gaagctgcta aagctgcagg
     2041 cgcagaactg gtaggtatgg aagatctggc tgaccagatc aagaaaggcg aaatgaactt
     2101 tgacgttgtt attgcttctc cggatgcaat gcgcgttgtt ggccagctgg gccaggttct
     2161 gggtccgcgc ggcctgatgc caaacccgaa agtgggtact gtaacaccga acgttgctga
     2221 agcggttaaa aacgctaaag ctggccaggt tcgttaccgt aacgacaaaa acggcatcat
     2281 ccacaccacc atcggtaaag tggactttga cgctgacaaa ctgaaagaaa acctggaagc
     2341 tctgctggtt gcgctgaaaa aagcaaaacc gactcaggcg aaaggcgtgt acatcaagaa
     2401 agttagcatc tccaccacca tgggtgcagg tgttgcagtt gaccaggctg gcctgagcgc
     2461 ttctgtaaac taatgccttt acgtgggcgg tgattttgtc tacaatctta cccccacgta
     2521 taatgcttaa tgcagacgta tatccgagat attcgggttg tggcaaggcg gcaactgagt
     2581 gagtcgccag gagcatagct aactatgtga ctggtgcgaa tgaaggaagc caacgccgcc
     2641 acaagttgaa tagcgacgga tagaaaagat ttgttcgttg gagcctggcc tatccaggcc
     2701 tccgtcgaag accgcaggag tttcgcaaga aacttaatcc cctgcgtaga cggtgacaga
     2761 acgctaagat tattctttta tattctggct tgtttctgct caccgtaatt aagacgctct
     2821 ctccgtttgg aggagtgaag tgagttccag agattttctc tggcaaacat ccaggagcaa
     2881 agctaatggc tttaaatctt caagacaaac aagcgattgt tgctgaagtc agcgaagtag
     2941 ccaaaggcgc gctgtctgca gtagttgcgg attcccgtgg cgtaactgta gataaaatga
     3001 ctgaactgcg taaagcaggt cgcgaagctg gcgtatacat gcgtgttgtt cgtaacaccc
     3061 tgctgcgccg tgctgttgaa ggtactccgt tcgagtgcct gaaagacgcg tttgttggtc
     3121 cgaccctgat tgcatactct atggaacacc cgggcgctgc tgctcgtctg ttcaaagagt
     3181 tcgcgaaagc gaatgcaaaa tttgaggtca aagccgctgc ctttgaaggt gagctgatcc
     3241 cggcgtctca gatcgaccgc ctggcaactc tgccgaccta cgaagaagca attgcacgcc
     3301 tgatggcaac catgaaagaa gcttcggctg gcaaactggt tcgtactctg gctgctgtac
     3361 gcgatgcgaa agaagctgct taatcgcagt tatcttttta acgcattcgc ttacgtataa
     3421 acttattctg atattcagga acaatttaaa tgtctatcac taaagatcaa atcattgaag
     3481 cagttgcagc tatgtctgta atggacgttg tagaactgat ctctgcaatg gaagaaaaat
     3541 tcggtgtttc cgctgctgct gctgtagctg tagctgctgg cccggttgaa gctgctgaag
     3601 aaaaaactga attcgacgta attctgaaag ctgctggcgc taacaaagtt gctgttatca
     3661 aagcagtacg tggcgcaact ggcctgggtc tgaaagaagc taaagacctg gtagaatctg
     3721 caccggctgc actgaaagaa ggcgtgagca aagacgacgc agaagcactg aaaaaagctc
     3781 tggaagaagc tggcgctgaa gttgaagtta aataagccaa cccttccggt tgcagcctga
     3841 gaaatcaggc tgatggctgg tgacttttta gtcaccagcc tttttgcgct gtaaggcgcc
     3901 agtagcgttt cacactgttt gactactgct gtgcctttca atgcttgttt ctatcgacga
     3961 cttaatatac tgcgacagga cgtccgttct gtgtaaatcg caatgaaatg gtttaagcgt
     4021 gatagcaaca ggcattgcgg aaagtgttcc attttccggt caacaaaata gtgttgcaca
     4081 aactgtccgc tcaatggaca gatgggtcga cttgtcagcg agctgaggaa ccctatggtt
     4141 tactcctata ccgagaaaaa acgtattcgt aaggattttg gtaaacgtcc acaagttctg
     4201 gatgtacctt atctcctttc tatccagctt gactcgtttc agaaatttat cgagcaagat
     4261 cctgaagggc agtatggtct ggaagctgct ttccgttccg tattcccgat tcagagctac
     4321 agcggtaatt ccgagctgca atacgtcagc taccgccttg gcgaaccggt gtttgacgtc
     4381 caggaatgtc aaatccgtgg cgtgacctat tccgcaccgc tgcgcgttaa actgcgtctg
     4441 gtgatctatg agcgcgaagc gccggaaggc accgtaaaag acattaaaga acaagaagtc
     4501 tacatgggcg aaattccgct catgacagac aacggtacct ttgttatcaa cggtactgag
     4561 cgtgttatcg tttcccagct gcaccgtagt ccgggcgtct tctttgactc cgacaaaggt
     4621 aaaacccact cttcgggtaa agtgctgtat aacgcgcgta tcatccctta ccgtggttcc
     4681 tggctggact tcgaattcga tccgaaggac aacctgttcg tacgtatcga ccgtcgccgt
     4741 aaactgcctg cgaccatcat tctgcgcgcc ctgaactaca ccacagagca gatcctcgac
     4801 ctgttctttg aaaaagttat ctttgaaatc cgtgataaca agctgcagat ggaactggtg
     4861 ccggaacgcc tgcgtggtga aaccgcatct tttgacatcg aagctaacgg taaagtgtac
     4921 gtagaaaaag gccgccgtat cactgcgcgc cacattcgcc agctggaaaa agacgacgtc
     4981 aaactgatcg aagtcccggt tgagtacatc gcaggtaaag tggttgctaa agactatatt
     5041 gatgagtcta ccggcgagct gatctgcgca gcgaacatgg agctgagcct ggatctgctg
     5101 gctaagctga gccagtctgg tcacaagcgt atcgaaacgc tgttcaccaa cgatctggat
     5161 cacggcccat atatctctga aaccttacgt gtcgacccaa ctaacgaccg tctgagcgca
     5221 ctggtagaaa tctaccgcat gatgcgccct ggcgagccgc cgactcgtga agcagctgaa
     5281 agcctgttcg agaacctgtt cttctccgaa gaccgttatg acttgtctgc ggttggtcgt
     5341 atgaagttca accgttctct gctgcgcgaa gaaatcgaag gttccggtat cctgagcaaa
     5401 gacgacatca ttgatgttat gaaaaagctc atcgatatcc gtaacggtaa aggcgaagtc
     5461 gatgatatcg accacctcgg caaccgtcgt atccgttccg ttggcgaaat ggcggaaaac
     5521 cagttccgcg ttggcctggt acgtgtagag cgtgcggtga aagagcgtct gtctctgggc
     5581 gatctggata ccctgatgcc tcaggatatg atcaacgcca agccgatttc cgcagcagtg
     5641 aaagagttct tcggttccag ccagctgtct cagtttatgg accagaacaa cccgctgtct
     5701 gagattacgc acaaacgtcg tatctccgca ctcggcccag gcggtctgac ccgtgaacgt
     5761 gcaggcttcg aagttcgaga cgtacacccg actcactacg gtcgcgtatg tccaatcgaa
     5821 acccctgaag gtccgaacat cggtctgatc aactctctgt ccgtgtacgc acagactaac
     5881 gaatacggct tccttgagac tccgtatcgt aaagtgaccg acggtgttgt aactgacgaa
     5941 attcactacc tgtctgctat cgaagaaggc aactacgtta tcgcccaggc gaactccaac
     6001 ctggatgaag aaggccactt cgtagaagac ctggtaactt gccgtagcaa aggcgaatcc
     6061 agcttgttca gccgcgacca ggttgactac atggacgtat ccacccagca ggtggtatcc
     6121 gtcggtgcgt ccctgatccc gttcctggaa cacgatgacg ccaaccgtgc attgatgggt
     6181 gcgaacatgc aacgtcaggc cgttccgact ctgcgcgctg ataagccgct ggttggtact
     6241 ggtatggaac gtgctgttgc cgttgactcc ggtgtaactg cggtagctaa acgtggtggt
     6301 gtcgttcagt acgtggatgc ttcccgtatc gttatcaaag ttaacgaaga cgagatgtat
     6361 ccgggtgaag caggtatcga catctacaac ctgaccaaat acacccgttc taaccagaac
     6421 acctgtatca accagatgcc gtgtgtgtct ctgggtgaac cggttgaacg tggcgacgtg
     6481 ctggcagacg gtccgtccac cgacctcggt gaactggcgc ttggtcagaa catgcgcgta
     6541 gcgttcatgc cgtggaatgg ttacaacttc gaagactcca tcctcgtatc cgagcgtgtt
     6601 gttcaggaag accgtttcac caccatccac attcaggaac tggcgtgtgt gtcccgtgac
     6661 accaagctgg ggccggaaga gatcaccgct gacatcccga acgtgggtga agctgcgctc
     6721 tccaaactgg atgaatccgg tatcgtttac attggtgcgg aagtgaccgg tggcgacatt
     6781 ctggttggta aggtaacgcc gaaaggtgaa actcagctga ccccagaaga aaaactgctg
     6841 cgtgcgatct tcggtgagaa agcctctgac gttaaagact cttctctgcg cgtaccaaac
     6901 ggtgtatccg gtacggttat cgacgttcag gtctttactc gcgatggcgt agaaaaagac
     6961 aaacgtgcgc tggaaatcga agaaatgcag ctcaaacagg cgaagaaaga cctgtctgaa
     7021 gaactgcaga tcctcgaagc gggtctgttc agccgtatcc gtgctgtgct ggtagccggt
     7081 ggcgttgaag ctgagaagct cgacaaactg ccgcgcgatc gctggctgga gctgggcctg
     7141 acagacgaag agaaacaaaa tcagctggaa cagctggctg agcagtatga cgaactgaaa
     7201 cacgagttcg agaagaaact cgaagcgaaa cgccgcaaaa tcacccaggg cgacgatctg
     7261 gcaccgggcg tgctgaagat tgttaaggta tatctggcgg ttaaacgccg tatccagcct
     7321 ggtgacaaga tggcaggtcg tcacggtaac aagggtgtaa tttctaagat caacccgatc
     7381 gaagatatgc cttacgatga aaacggtacg ccggtagaca tcgtactgaa cccgctgggc
     7441 gtaccgtctc gtatgaacat cggtcagatc ctcgaaaccc acctgggtat ggctgcgaaa
     7501 ggtatcggcg acaagatcaa cgccatgctg aaacagcagc aagaagtcgc gaaactgcgc
     7561 gaattcatcc agcgtgcgta cgatctgggc gctgacgttc gtcagaaagt tgacctgagt
     7621 accttcagcg atgaagaagt tatgcgtctg gctgaaaacc tgcgcaaagg tatgccaatc
     7681 gcaacgccgg tgttcgacgg tgcgaaagaa gcagaaatta aagagctgct gaaacttggt
     7741 gacctgccga cttccggtca gatccgcctg tacgatggtc gcactggtga acagttcgag
     7801 cgtccggtaa ccgttggtta catgtacatg ctgaaactga accacctggt cgacgacaag
     7861 atgcacgcgc gttccaccgg ttcttacagc ctggttactc agcagccgct gggtggtaag
     7921 gcacagttcg gtggtcagcg tttcggggag atggaagtgt gggcgctgga agcatacggc
     7981 gcagcataca ccctgcagga aatgctcacc gttaagtctg atgacgtgaa cggtcgtacc
     8041 aagatgtata aaaacatcgt ggacggcaac catcagatgg agccgggcat gccagaatcc
     8101 ttcaacgtat tgttgaaaga gattcgttcg ctgggtatca acatcgaact ggaagacgag
     8161 taattctcgc tcaaacaggt cactgctgtc gggttaaaac ccggcagcgg attgtgctaa
     8221 ctccgacggg agcaaatccg tgaaagattt attaaagttt ctgaaagcgc agactaaaac
     8281 cgaagagttt gatgcgatca aaattgctct ggcttcgcca gacatgatcc gttcatggtc
     8341 tttcggtgaa gttaaaaagc cggaaaccat caactaccgt acgttcaaac cagaacgtga
     8401 cggccttttc tgcgcccgta tctttgggcc ggtaaaagat tacgagtgcc tgtgcggtaa
     8461 gtacaagcgc ctgaaacacc gtggcgtcat ctgtgagaag tgcggcgttg aagtgaccca
     8521 gactaaagta cgccgtgagc gtatgggcca catcgaactg gcttccccga ctgcgcacat
     8581 ctggttcctg aaatcgctgc cgtcccgtat cggtctgctg ctcgatatgc cgctgcgcga
     8641 tatcgaacgc gtactgtact ttgaatccta tgtggttatc gaaggcggta tgaccaacct
     8701 ggaacgtcag cagatcctga ctgaagagca gtatctggac gcgctggaag agttcggtga
     8761 cgaattcgac gcgaagatgg gggcggaagc aatccaggct ctgctgaaga gcatggatct
     8821 ggagcaagag tgcgaacagc tgcgtgaaga gctgaacgaa accaactccg aaaccaagcg
     8881 taaaaagctg accaagcgta tcaaactgct ggaagcgttc gttcagtctg gtaacaaacc
     8941 agagtggatg atcctgaccg ttctgccggt actgccgcca gatctgcgtc cgctggttcc
     9001 gctggatggt ggtcgtttcg cgacttctga cctgaacgat ctgtatcgtc gcgtcattaa
     9061 ccgtaacaac cgtctgaaac gtctgctgga tctggctgcg ccggacatca tcgtacgtaa
     9121 cgaaaaacgt atgctgcagg aagcggtaga cgccctgctg gataacggtc gtcgcggtcg
     9181 tgcgatcacc ggttctaaca agcgtcctct gaaatctttg gccgacatga tcaaaggtaa
     9241 acagggtcgt ttccgtcaga acctgctcgg taagcgtgtt gactactccg gtcgttctgt
     9301 aatcaccgta ggtccatacc tgcgtctgca tcagtgcggt ctgccgaaga aaatggcgct
     9361 ggagctgttc aaaccgttca tctacggcaa gctggaactg cgtggtcttg ctaccaccat
     9421 taaagctgcg aagaaaatgg ttgagcgcga agaagctgtc gtttgggata tcctggacga
     9481 agttatccgc gaacacccgg tactgctgaa ccgtgcaccg actctgcacc gtctgggtat
     9541 ccaggcattt gaaccggtac tgatcgaagg taaagctatc cagctgcacc cgctggtttg
     9601 tgcggcatat aacgccgact tcgatggtga ccagatggct gttcacgtac cgctgacgct
     9661 ggaagcccag ctggaagcgc gtgcgctgat gatgtctacc aacaacatcc tgtccccggc
     9721 gaacggcgaa ccaatcatcg ttccgtctca ggacgttgtt ctgggtctgt actatatgac
     9781 ccgtgactgt gttaacgcca aaggcgaagg catggtgctg actggcccga aagaagcaga
     9841 acgtctgtat cgctctggtc tggcttctct gcatgcgcgc gttaaagtgc gtatcaccga
     9901 gtatgaaaaa gatgcaaacg gtgaattagt agcgaaaacc agcctgaaag acacgactgt
     9961 tggccgtgcc attctgtgga tgattgtacc gaaaggtctg ccttactcca tcgtcaacca
    10021 ggcgctgggt aaaaaagcaa tctccaaaat gctgaacacc tgctaccgca ttctcggtct
    10081 gaaaccgacc gttatttttg cggaccagat catgtacacc ggcttcgcct atgcagcgcg
    10141 ttctggtgca tctgttggta tcgatgacat ggtcatcccg gagaagaaac acgaaatcat
    10201 ctccgaggca gaagcagaag ttgctgaaat tcaggagcag ttccagtctg gtctggtaac
    10261 tgcgggcgaa cgctacaaca aagttatcga tatctgggct gcggcgaacg atcgtgtatc
    10321 caaagcgatg atggataacc tgcaaactga aaccgtgatt aaccgtgacg gtcaggaaga
    10381 gaagcaggtt tccttcaaca gcatctacat gatggccgac tccggtgcgc gtggttctgc
    10441 ggcacagatt cgtcagcttg ctggtatgcg tggtctgatg gcgaagccgg atggctccat
    10501 catcgaaacg ccaatcaccg cgaacttccg tgaaggtctg aacgtactcc agtacttcat
    10561 ctccacccac ggtgctcgta aaggtctggc ggataccgca ctgaaaactg cgaactccgg
    10621 ttacctgact cgtcgtctgg ttgacgtggc gcaggacctg gtggttaccg aagacgattg
    10681 tggtacccat gaaggtatca tgatgactcc ggttatcgag ggtggtgacg ttaaagagcc
    10741 gctgcgcgat cgcgtactgg gtcgtgtaac tgctgaagac gttctgaagc cgggtactgc
    10801 tgatatcctc gttccgcgca acacgctgct gcacgaacag tggtgtgacc tgctggaaga
    10861 gaactctgtc gacgcggtta aagtacgttc tgttgtatct tgtgacaccg actttggtgt
    10921 atgtgcgcac tgctacggtc gtgacctggc gcgtggccac atcatcaaca agggtgaagc
    10981 aatcggtgtt atcgcggcac agtccatcgg tgaaccgggt acacagctga ccatgcgtac
    11041 gttccacatc ggtggtgcgg catctcgtgc ggctgctgaa tccagcatcc aggtgaaaaa
    11101 caaaggtagc atcaagctca gcaacgtgaa gtcggttgtg aactccagcg gtaaactggt
    11161 tatcacttcc cgtaatactg aactgaaact gatcgacgaa ttcggtcgta ccaaagaaag
    11221 ctacaaagta ccttacggtg cggtactggc gaaaggcgat ggcgaacagg ttgctggcgg
    11281 cgaaaccgtt gcaaactggg acccgcacac catgccggtt atcaccgaag taagcggttt
    11341 tgtacgcttt actgacatga tcgacggtca gaccattact cgtcagacag acgaattgac
    11401 cggtctgtct tcgctggtgg ttctggattc cgcagaacgt accgcaggtg gtaaagatct
    11461 gcgtccggca ctgaaaatcg ttgatgctca gggtaacgac gttctgatcc caggtaccga
    11521 tatgcctgcg cagtacttcc tgccgggtaa agcgattgtt cagctggaag atggcgtaca
    11581 gatcagctct ggtgacaccc tggcgcgtat tccgcaggaa tccggcggta ccaaggacat
    11641 caccggtggt ctgccgcgcg ttgcggacct gttcgaagct cgtcgtccga aagagccggc
    11701 aatcctggct gaaatcagcg gtatcgtttc cttcggtaaa gaaaccaaag gtaaacgtcg
    11761 tctggttatc accccggtag acggtagcga tccgtacgaa gagatgattc cgaaatggcg
    11821 tcagctcaac gtgttcgaag gtgaacgtgt agaacgtggt gacgtaattt ccgacggtcc
    11881 ggaagcgccg cacgacattc tgcgtctgcg tggtgttcat gctgtgactc gttacatcgt
    11941 taacgaagta caggacgtat accgtctgca gggcgttaag attaacgata aacacatcga
    12001 agttatcgtt cgtcagatgc tgcgtaaagc taccatcgtt aacgcgggca gctccgactt
    12061 cctggaaggc gaacaggttg aatactctcg cgtcaagatc gcaaaccgcg aactggaagc
    12121 gaacggcaaa gtgggtgcaa cttactcccg cgatctgctg ggtatcacca aagcgtctct
    12181 ggcaaccgag tccttcatct ccgcggcatc gttccaggag accactcgcg tgctgaccga
    12241 agcagccgtt gcgggcaaac gcgacgaact gcgcggcctg aaagagaacg ttatcgtggg
    12301 tcgtctgatc ccggcaggta ccggttacgc gtaccaccag gatcgtatgc gtcgccgtgc
    12361 tgcgggtgaa gctccggctg caccgcaggt gactgcagaa gacgcatctg ccagcctggc
    12421 agaactgctg aacgcaggtc tgggcggttc tgataacgag taatcgttaa tccgcaaata
    12481 acgtaaaaac ccgcttcggc gggttttttt atggggggag tttagggaaa gagcatttgt
    12541 cagaatattt aaggaatttc tgaatactca taatcaatgt agagatgact aatatcctga
    12601 aactgactga actaattgag tcaaactcgg caaggattcg atactattcc tgtgtaactt
    12661 tcttaaggaa cgagaatgaa acaggaagtg gaaaagtggc gaccttttgg acatccggat
    12721 ggtgatattc gtgatttatc atttcttgat gctcatcagg ctgtctacgt tcagcatcat
    12781 gagggcaaag agcctttaga gtatcgcttt tgggttacct actctcttca ctgcttcaca
    12841 aaagattatg aacatcagac gaacgaagaa aaacaatcgt taatgtacca cgcgcctaaa
    12901 gaatctcgtc ccttctgcca gcaccgttat aacttagcgc gcacacactt aaaaagaact
    12961 attttggcgc tgccagaaag caacgttatt catgccgggt atggtagcta tgccgtgatt
    13021 gaggtggact tagacggagg agataaggca ttttactttg ttgcgttcag ggctttcagg
    13081 gaaaagaaaa aactccgttt gcatgtaact agcgcttatc ccatttctga aaaacagaaa
    13141 ggtaaatcag tgaaattttt caccattgcc tacaacttat tgagaaataa gcagcttcct
    13201 cagtcctcaa aataacaaaa cccaccttaa ggtgggtttc gccagagaat tatctctggt
    13261 attcagaacg ccattaccgg actttgcctt gaccttgcga taatcgcagg ttgcgggatg
    13321 tctgaatttc ttcagtctgc tgcatcctgg aagatgagaa catgtgttct tattttcgtc
    13381 tctatcatag ttgagtattt actctcttac aatcagatct ctttcattgc tcaacaggcg
    13441 atggcttcag actttgcatt acggaatttt taagaaaggc agggcgaaac gaggaagaag
    13501 cttttcgccc tggacaacat tcctgctgac ggcactacca taaaaaaaca gaacgctttc
    13561 aacaaccttg cgtttttcat cttccgtagc ggctcggaat aaatttacgt tcttacattc
    13621 cgtctcatag tctttgcgag gcccgtccca gatagctgtc ccagtctttc cataccggct
    13681 gcaaaccctg agcggttaac gcggcagcaa ccgcttccgg tctgcgatcg tcgtgcggtg
    13741 agaactgttc caactcgggg tgattatcgg catagccacc tggctgcgtt ttcgagaagg
    13801 cgctgacgtt attgatcgcc agcggaataa cgcgatcgcg aaaccacggt gattcccgcg
    13861 tggagagtga cagttcaatc tccggtgcaa gcagtcggaa ggcgcagatg gtttgcacta
    13921 actggcgttc atccataatc gacgcaggct caatgccgcc agtacacggg cgcaggcgcg
    13981 gaaaggagac agagtaacgg ctttgccagt aatgctgttg cagccatagc aaatgttcgg
    14041 caaccatata gcagtcaacg cgccagttgt cggaaaggcc aattagcgcg ccgaggccta
    14101 tcttatcaat ccccgcacgc cccagccgat ccggcgtttc cagccgccag aagaagtcct
    14161 gtttttttcc tttcagatga tggcgggcat aagtcgcctc gtgatatgtc tcctgataaa
    14221 ccatcacgcc atccagacca agctgcttta actcggcgta ttccgtctcc gccagcggtt
    14281 gcacttccat ttgtagtgaa gagaactgtt cacgcagggc ggggagatga cgacgaaagt
    14341 aatccatccc cactttcgcc tgatgttcac cagtgactaa tagcagatgt tcaaagccca
    14401 tctcccgtat agcggcgctt tccctggcaa tatccgcttc atccagcgtt ttgcgcttga
    14461 tgcgattact catggaaaat ccgcagtacg tgcagtcgtt agcgcaaaga ttggaaagat
    14521 aaagcgggac gtagaaacta actgtgttgc caaatcgctg acgggtcaga cgctgcgccc
    14581 gttgggccag ttgttccaga tagccactgg cggctggcga taacagcgcc atcatatcgt
    14641 cgcgggtgag ttgcgaggca tttagcgccc gttctacgtc agcagccgtt ttgccgttga
    14701 tacgcaggcg gatgtcgtcc cagtccagtt gtcgccagcg atcgctgaag gttttcatgc
    14761 cgatgcctcc agaaatccag tcagcgggct ggtggcatga gcaaaatgac tgcggctgcc
    14821 cggtccggac tgacgtgcca gcaggcctgc ttctaccgcc agacgaaatg ccttcgccat
    14881 gttgacggga tcgtccgcga cggcaattgc cgtattcact aacaccgcgt cggcccccat
    14941 ttccagcgcc tgcgcggcat ggctgggaac gccgatgcca gcatcgacaa ccaccggcac
    15001 tgtggcctgc tggataataa tctccagcat ggcgcgggtt tccagtccct gattcgagcc
    15061 aatcggcgcg ccgagcggca tcaccgctgc acagccgact tcttccagac gtttacacaa
    15121 taccggatcg gccccgcagt aaggcagcac gacaaatccc tgttgtacca gcgtttcggc
    15181 ggctttcagg gtttcgatgg gatcgggcaa cagccagcgg gcgtcagggt gaatctctaa
    15241 ttttaaccag tttgtgccta acgcttcacg agccagatgg gcggcgaaaa tggcttcttc
    15301 cgctgttttc gccccggatg tatttggcag cagggtcaca cccgccgcga taagcggttc
    15361 gaggatagcg tcgttgtgct ggcgcaagtc gacacgtttc atcgccagtg tcaccagctg
    15421 gctgccggaa gcgcggatcg cctccaccat cagttgtgaa gaagcgaatt tccctgtgcc
    15481 ggtaaacaga tgtgaatcaa acgttttgtc cgcaatacgt aacatttcaa ccccctgcaa
    15541 taacctgaaa aagcaggatc tggtcgccat cctgcacgat atgttgcgcc cactgctcac
    15601 gcgggacgat ttgctgatta atcgccagag ccgcgcccgc ttgtcgttgg tcgagttgct
    15661 ccagtagttc gtgaacagtt tgcccggcgg cgcactgcat cggttgatcg ttaaacagga
    15721 tctgcattgc ttcctccgca taccgggcaa ccactggcgc ggcgcaacgc caggctgcgc
    15781 cactggctcg atttaccgtc gaacagtcgg agttctcccg caggtgtctc tataccgctt
    15841 aataacttaa tggcttccag tgcctgcaaa gtgcccataa ccccgaccac cgggccaacc
    15901 acgcccgccg tgcggcagtt gcgttctggc tcctggttat ctggccacag gcagcggtaa
    15961 cacccctgct cccagggcgg cgtcagtacc atcaactgac cgccaaatcc gaccgcgctg
    16021 gcggtgataa gcggcgtgtt gagtgccacg caggcggcat taatctcctg gcgagtcgcc
    16081 atattgtcgg tacagtcgag caccacatcg gcccgtgcaa ccgcatcttt taacgcctca
    16141 cccgttaacc gttgttgtaa tgctgtcagt tgaatgtcgg gattcaactg tgtcagtcgc
    16201 tgttggctga cctgcgattt cgggcgatcg atatcttcag tggtaaagag gatttgtcgt
    16261 tgcagattgc ttaaatgcac atcgtcgtca tctgccagta ccagcgtccc gacgccagcg
    16321 cccgccaggt acagcgcagc aggtgtaccc agcccgccca gaccgataat cagcacctgg
    16381 ctgtcgagca gtttttgctg cccgtccaga gcgatatcgt cgagcaggat ttggcggcta
    16441 taacgcataa agtcacggtc attcatcgcc aactcctgca atttccagca actgtgccgt
    16501 tgccaaacgc cagtctgcag cttgagtaat ggcgctgacg acggcgatac tgccgacacc
    16561 cgttgctatc accgcaggcg cgcgtgccag actgataccg ccaatcgcca cggtgggata
    16621 atccgccagt cgctcaacat gccgtgccag ctgttccagc ccctgcggtg cagaaggcat
    16681 ctgtttggtt tgcgtcggga acacatgtcc cagcgcgata taagaggggc gtgctgccag
    16741 cgcgacgtcg atttccatat cgtcatgtgt cgaaacgccc agccgcaggc ctgccgcgcg
    16801 gatggcattg agatcggtgg cttgcaaatc ttcctgcccc aaatggacgc catacgcctg
    16861 atgcttgatc gccagccgcc agtaatcgtt gataaacaat cgcgcgttat agcggcggcc
    16921 cagcgcaatt gccgccacga catcggcttc cacctcttca tcgcgccgat ctttgatgcg
    16981 tagctggaga gtacgtacgc ctgcatccaa cagacgttcg atccactgta cgctgtccac
    17041 caccgggtac agtcctgaac gaaaaggtac aggaggaaaa tcaggctgat acatcacgct
    17101 tcctccttac gcaggtagat ttctccgcct ctggcacgga agttctccga catatccgcc
    17161 attcccattt caatagtttg cgtggcggcg taatcacgca cttcctggct gattttcatc
    17221 gagcagaatt tcggcccaca catggagcaa aaatgggcga ctttacctga ctcttgcggc
    17281 agggtttcat cgtgataagc gcgggcggta aacgggtcga gggccagatt aaactggtct
    17341 tcccagcgaa attcgaagcg ggctttcgac atggcgttat cgcgaatttg cgcgcccgga
    17401 tgccctttcg ccaggtcagc ggcgtgggcg gcaatcttat aggtgataag cccctgctta
    17461 acatcttctt tattgggcag acccagatgc tcttttggcg ttacgtaaca gagcatcgcg
    17521 cagccaaacc agccaatcat cgccgcacca atccccgacg tgaagtggtc atagcccggc
    17581 gcaatatcgg tagttagcgg ccccagagtg taaaacggcg cttcgtggca gtgctctaac
    17641 tcctcggtca tattgcggcg gatcatctgc atcggcacgt ggcctgggcc ttcaatcatc
    17701 acctgcacgt catattccca ggcaattttg gtcagttcgc ccagcgtatg cagctcggca
    17761 aactgcgctt catcgttggc gtcctgaata gaaccggggc gcagaccgtc gcccagcgac
    17821 agcgaaacgt cataagcggc acagatttca caaatttcgc ggaagtgttg atagaggaaa
    17881 ttttcctgat gatgggagag gcaccatttc gccataatcg aaccgccgcg agagacgata
    17941 ccggtcaggc gtttcgcggt catcggcaca tagcgcagca gtacgcccgc atggatagtg
    18001 aagtaatcca caccttgctc ggcctgttcc agcagcgtgt cgcggaacgc ttcccaggta
    18061 agatcttcgg cgatcccgtt aaccttctcc agcgcctggt agatcggcac tgtaccgatc
    18121 ggcaccgggc tgttacgcaa aatccactcg cgggtttcgt gaatatagcg accggtggag
    18181 agatccatca ccgtatccgc tccccagcgc gtggaccata ccagcttttc cacttcttct
    18241 tcgatggaag aggtgaccgc cgagttgccg atattggcgt taacttttac caggaaattg
    18301 cgaccaataa tcatcggctc cgattccgga tgattaatgt tggccgggat aatcgcacgt
    18361 ccggcagcaa cttcatcacg gacaaattcc gcagtgatat tttccggcag acgtgcgcca
    18421 aagctcattc ccggatgctg gtggcgtaaa acttcgctac ggatgcgctc gcggcccata
    18481 ttctcgcgga tggcgatgaa ttccatttcc ggcgtgatga tgccctggcg ggcgtagtgc
    18541 agttgggtga cacggcgtcc tgctttggcg cgttttggtg ttagtacgcc gctaaaacgc
    18601 agttcgtcga ggccatcatc tgccagccgc gctttagtgt aatcggaact gcgcacggta
    18661 agttcttcgg tatcgccgcg cgcatcgatc cacggctggc gtagttttgc cagcccttgc
    18721 tgcacgttaa tggcaatctg cggatcacca tacgggccgg aggtgtcgta gaccggaatc
    18781 gcttcgtttt cttcgtactg cggctgttct ttgctaccgc caattagcgt cgggctaacc
    18841 tggatctcac gcatcggcac gcgcacgccg gggtgtgtgc cagtgatata aatgcgtttt
    18901 gagttgggaa aggcggtgcc ttccagggtg tcgataaaat gttgggcccg ggcgcgttgt
    18961 tcgcggcggg tcagttttgt tgcagacata gctcattcca aaaagttaag gacgtggctt
    19021 gtcagacgac ggatgaagca agagacgatc gccgcagggg cgatgcgata gcagattaac
    19081 tcttgttccc ttcgcaggta ttagcctgat caggttccgc ggatcccgaa taaacggtct
    19141 cagccagtta aggcactccg acaagaaatt agccccgcaa atggggcatt gaatgtaaat
    19201 tacgcgttaa cagcgcagaa ctcaagcagg atgtttgacg cgggcggcgt caagcaccag
    19261 cagaatcagc ttatcttcca gcacaaagcg cgcttccagc gcctcgccga tgtcagataa
    19321 aacctgttga aactcaaggt aattatcatg atcgatggcg gtttccagac tggaatcgta
    19381 gtaatccata atctgttgag tattggcttc gagttgcggc caaatcttcg cggcgcgcgc
    19441 gagttgcccg ttgccttcca gcttatgaag aatgcgctca taaatactga aatgtccggc
    19501 agacaagtaa tcgaccaggc tctgacaaaa atcatcaagg gctttttcgt ttagcctcat
    19561 gtacgattct ttgccaggct taatgccaac cagattgtag taagccacga gcagatgctt
    19621 acgtacatgt agccagcgat caaccagttt gttacttcct ctgacgcgct ccgtcaggtt
    19681 atcgagctgg ttaagcatga ttgactccgc aagtttgtat tcaaaaactg ctcagtgaga
    19741 aatgtaaaaa ccatgttaaa catgccagtg atgcaaaggt agtgcaagag ctatggatcg
    19801 tataattgaa aaattagatc acggctggtg ggtcgtcagc catgaacaaa aattatggtt
    19861 gccgaaggga gaattgccat atggcgaagc ggcaaatttc gatcttgtgg gtcagcgcgc
    19921 actacaaatc ggcgaatggc agggggaacc tgtttggtta gtacaacagc agcggcgtca
    19981 cgatatgggg tcggtacgtc aggtcattga tctcgatgtt gggctgtttc aactggccgg
    20041 acgaggcgta caactggcgg agttttaccg atcgcataaa tactgtggtt actgcgggca
    20101 tgaaatgtat ccgagcaaaa ccgaatgggc gatgctgtgc agccattgcc gtgagcgtta
    20161 ctacccgcaa atcgccccct gcattattgt tgccatccgt cgcgatgatt cgatcctcct
    20221 cgcccagcat acccgccatc gtaacggtgt ccatacagta cttgccggat tcgtcgaagt
    20281 gggcgaaacc ctcgagcagg cagtcgcgcg ggaagtgatg gaagagagcg gaattaaagt
    20341 taaaaacttg cgttacgtga cttctcagcc gtggccgttt cctcagtctt taatgaccgc
    20401 gtttatggcg gaatatgaca gcggcgacat cgtgatcgac ccgaaagaat tgctcgaggc
    20461 gaactggtat cgctatgacg atttgccgtt actcccgccg cccggcaccg tagcgcgccg
    20521 tctgatagaa gatacggtgg cgatgtgtcg ggcagagtat gagtgatgat acactgaccg
    20581 cctgacgcac taaggaacag ccaaaatgac cgaacttaaa aacgatcgtt atctgcgggc
    20641 gctgctgcgc cagcccgttg atgtcactcc agtatggatg atgcgccagg cgggtcgcta
    20701 tctaccggaa tataaagcca cgcgcgccca ggcgggcgat tttatgtcgc tgtgcaaaaa
    20761 cgccgagctg gcgtgcgaag tgactttgca accgctgcgt cgctacccgc tggatgcggc
    20821 gatcctcttt tccgatatcc tcaccgtgcc ggacgcgatg gggttagggc tctattttga
    20881 agccggagaa ggtccgcgtt ttacctcgcc agtcacctgc aaagccgacg tcgataaact
    20941 gccaattccg gacccggaag atgagctggg ttacgtgatg aacgcggtgc gtaccattcg
    21001 tcgcgaactg aaaggcgaag tgccgctgat tggtttttcc ggcagcccgt ggacgctggc
    21061 gacctacatg gtggaaggcg gcagcagcaa agccttcacc gtgatcaaaa aaatgatgta
    21121 tgccgatccg caggcgctgc acgctctgct cgataaactg gcgaaaagcg tcactttgta
    21181 tctgaatgcg cagattaaag ccggtgctca ggcagtgatg attttcgaca cctggggcgg
    21241 cgtgcttacc gggcgcgatt atcaacagtt ctcgctctat tacatgcata aaattgttga
    21301 tggtttagtg cgtgaaaacg acggtcgccg cgtaccggtc acgctgttta ccaaaggcgg
    21361 cggacagtgg ctggaagcca tggcagaaac cggttgcgat gcgctgggcc tcgactggac
    21421 aacggacatc gccgatgcgc gccgccgtgt gggcaataaa gtcgcgttgc agggtaatat
    21481 ggatccgtcg atgctgtacg cgccgcctgc ccgcattgaa gaagaagtag cgactatact
    21541 tgcaggtttc ggtcacggcg aaggtcatgt ctttaacctt ggtcacggca ttcatcagga
    21601 tgtgccgcca gaacatgctg gcgtgttcgt ggaggcagtg catcgactgt ctgaacagta
    21661 tcaccgctaa ggagtgatta tggatctcgc gtcattacgc gctcaacaaa tcgaactggc
    21721 ttcttctgtg atccgcgagg atcgactcga taaagatcca ccggatctga tcgccggagc
    21781 cgatgtcggg tttgagcagg gcggagaagt gacgcgagcg gcgatggtgc tgctgaaata
    21841 tccctcgctt gagctggtgg agtataaagt tgcccgcatc gccaccacca tgccttacat
    21901 tccaggtttt ctttccttcc gcgaatatcc tgcgctgctg gcagcgtggg agatgctgtc
    21961 gcaaaagccg gatttagtgt ttgtcgatgg tcatgggatc tcgcatcctc gccgtcttgg
    22021 cgtcgccagc cattttggct tattggtgga tgtgccgacc attggcgtgg cgaaaaaacg
    22081 tctctgcggt aaattcgaac cgctctccag cgaaccgggc gcgctggccc cgctgatgga
    22141 taaaggcgag cagctggcct gggtctggcg cagcaaagcg cgctgtaacc cgttgtttat
    22201 cgctaccggc catcgggtca gcgtggacag cgcgctggcg tgggtacaac gctgcatgaa
    22261 aggctatcgt ctgccggagc caacgcgctg ggcggacgcg gtggcctcgg aacgtccggc
    22321 gttcgtgcgc tatacagcaa atcagcccta attcaggtaa actgcggata atttccgtat
    22381 ttgagaactc aacatgttac aaaacccaat tcatctgcgt ctggagcgcc tggaaagctg
    22441 gcagcatgtc actttcatgg cttgcttatg cgaacgcatg taccccaatt acgccatgtt
    22501 ctgccagcaa accggttttg gtgatgggca aatttaccgt cgtattctcg atctcatctg
    22561 ggaaacgctg accgttaaag acgcaaaagt aaatttcgac agccaactgg agaaatttga
    22621 agaagcgatt ccttcagccg acgatttcga tctgtacggc gtttatccgg caatcgatgc
    22681 ctgcgtggcg ttaagtgaac tggtccattc gcgtttgagt ggtgaaacgc tcgaacacgc
    22741 ggtggaagtg agtaagacct ccatcacgac cgttgcgatg ctggaaatga ctcaggctgg
    22801 tcgcgaaatg agcgatgaag agcttaaaga aaacccagct gtagagcaag aatgggacat
    22861 tcagtgggaa atattccgac ttttagctga gtgcgaagaa cgcgatatcg agctgataaa
    22921 aggccttagg gcagacctgc gtgaggcggg tgagagcaat attggtataa tttttcagca
    22981 ataagaccag aaaacgtgat ttaacgcctg atttgtcgta cctggagtct tccctttcgc
    23041 cccccgtctg gtctacattt ggggggcgaa aaaaagtggc tatcggtgcg tgtatgcagg
    23101 agagtgctat tctggcattt ccgtcgcact cgatgcttag caagcgataa acacattgta
    23161 aggataactt atgaacaaga ctcaactgat tgatgtaatt gcagagaaag cagaactgtc
    23221 caaaacccag gctaaagctg ctctggagtc cactctggct gcaattactg agtctctgaa
    23281 agaaggcgat gctgtacaac tggttggttt cggtaccttc aaagtgaacc accgcgctga
    23341 gcgtactggc cgcaacccgc agaccggtaa agaaatcaaa attgccgcag ctaacgtacc
    23401 ggcatttgtt tctggcaagg cactgaaaga cgcagttaag taagattgcg tggcagtgaa
    23461 cagttttaac gaaggggtgg tttcacccct tttgtctttc tggcgtcgat cattgatgct
    23521 ggcaggtgcg ctgtttctca ctgcctgtag ccacaactct tcacttcctc cctttaccgc
    23581 cagtggattt gctgaagacc agggcgcggt acgcatctgg cgaaaagaca gcggcgataa
    23641 tgtgcatctg cttgccgtgt ttagcccgtg gcgcagtggc gataccacga cgcgagagta
    23701 tcgctggcag ggcgataacc tcacgctcat caatatcaat gtttacagca aaccgccggt
    23761 gaacattcgc gcgcgttttg acgatcgcgg tgatctgagc tttatgcaac gtgaatccga
    23821 tggggaaaag cagcagcttt ctaacgacca aatcgattta taccgttatc gtgctgctca
    23881 gatccgccag attagcgatg ccttacgtca ggggagagtc gtgctgcgcc aggggcgctg
    23941 gcatgcgatg gaacagaccg tgaccacctg cgaagggcaa accattaaac ctgatttaga
    24001 ttcgcaggcg atagcgcata tcgagcgccg ccagagccgc tcttctgttg atgtcagcgt
    24061 ggcatggctg gaagcgcccg aaggttcgca attactgtta gtggcaaact ctgatttctg
    24121 tcgctggcaa cccaacgaga aaacgttctg atttaccagt ggcccatacc catatgaccg
    24181 ccaccaccgc agccgccgta gcccatgccc attccggctc cgcgcggaat acccgcttca
    24241 gccatcgcga tatcccgttt cacccgtaac tcatctaacg actgacgcaa attctccatc
    24301 tctttggcga ccgcgttaat tttgctgcta tccggtgggt tcgcggctaa cagggcattg
    24361 tattcataac gcttcgtcac cagttgctgt tgcagtgcgc tgctttgagt gtaaaagtca
    24421 ttatggattt tctgccacgc tgtctgttgt tcgctggtca aaggcgcggc attttgctgc
    24481 cacataccgt gtccgccgtg agcaaatgca gatgtcgatc ccatcgccat tgctgaaagc
    24541 gccatcatta ccagggcaat tttcgtgttc cgtttcatgg ttaatcctcc agtggttgtc
    24601 ttacttcggg tattgcatct tccgtgccaa cgatgaaacg ctgatatgac gggtaatctg
    24661 gcatgataaa cgagtaaaaa tgactcgcct gctgcgggta gcgagtcatt tttactcatt
    24721 gaaacttaag cctttgtgtt acagcgcagg gtaagcgctg ataaaagatg gcatgatttc
    24781 tgctgtcaga aagggatgag caggcaaaga agaagatgcg ttttatgcaa cgttctaaag
    24841 actccttagc taaatggtta agcgcgatcc tccccgtggt cattgttggg ctggtagggt
    24901 tgtttgcggt gacggtgatt cgcgattatg ggcgcgagac tgccgccgcc agacaaacgc
    24961 tgctggaaaa aggcagtgta cttattcgcg ctcttgaatc cggctcgcgc gtcggcatgg
    25021 ggatgcgcat gcatcatgcg cagcagcagg ccttactgga agaaatggcc gggcagcctg
    25081 gagtacgttg gtttgcggtc acggatgaac aaggaacaat cgtgatgcat agcaactccg
    25141 gcatggtggg aaaacagctt tattccccgc aggaaatgca gcagttacat ccgggagatg
    25201 aagaagcgtg gcggcggatc gatagcgcag acggcgagcc tgttctggaa atttatcgcc
    25261 agtttcaacc gatgtttgct gctggaatgc accggatgcg ccatatgcag cagtatgccg
    25321 cgacaccaca agcaattttc atcgctttcg acgccagtaa tattgtgagt gccgaagatc
    25381 gtgagcagag aaacaccctg attatcctct tcgccctggc gacggtcttg ctggcaagcg
    25441 tattgtcatt cttctggtat cgccgctatc tgcgctcgcg ccagcttcta caagatgaaa
    25501 tgaagcgcaa agagaagcta gtggcgctgg ggcatcttgc ggcaggcgtt gcccacgaaa
    25561 tccgtaaccc actttcctcg attaaaggac tggcgaaata ctttgccgag cgcgcgcctg
    25621 cagggggaga agcgcatcaa ctggcgcagg tgatggcgaa agaggccgac cgtttaaacc
    25681 gcgtggtaag cgagctgctg gaactggtta agccaacgca tctggctttg caggcggtgg
    25741 atctcaacac gctgattaac cactcattac agctggtagg ccaggatgca aacagccggg
    25801 agatccagtt acgctttacc gccaacgaca cattaccgga aattcaggcc gatccggaca
    25861 ggctgactca ggtcctgttg aatctctatc tcaatgctat tcaggcgatt ggtcagcatg
    25921 gcgtgattag cgtgacggtc agcgaaagcg gcgcgggcgt gaaaatcagc gttaccgaca
    25981 gcggtaaggg aattgcggca gatcagcttg aagccatctt cactccgtac ttcaccacca
    26041 aagccgaagg caccggattg gggctggcgg tcgtgcataa tattgttgaa caacacggtg
    26101 gtacaattca ggtcgcaagc caggagggaa aaggctcaac gttcaccctc tggcttccgg
    26161 tcaatattac gcgtaaggac ccacaaggat gacgcacgat aatatcgata ttctggtggt
    26221 ggatgatgac attagccact gcactatttt gcaggcttta ctgcgcggct ggggctataa
    26281 cgtcgcgctg gcgaacagcg ggcgacaggc gctggagcag gtgcgggagc gggtttttga
    26341 tcttgtgctt tgcgatgtgc gaatggcaga gatggacggc attgccacgc taaaagagat
    26401 caaagcgtta aatccggcga ttccggtact gattatgacc gcatactcca gcgtcgagac
    26461 ggcggtagag gcactgaaaa ccggggcgct ggattatctc atcaagccgc tggatttcga
    26521 caccctgcag gcgacgctgg aaaaggcgct cgcgcatacg cacagtattg atgctgaaac
    26581 gcctgcggtg actgccagcc agttcggtat ggtcggtaaa agcccggcga tgcaacacct
    26641 gctcagtgaa atcgccctcg tcgcgccatc ggaagccacg gtgctgatcc acggcgattc
    26701 cggcaccggt aaagagctgg tcgccagggc gattcacgcc agtagcgcac gtagcgaaaa
    26761 accgctggta acgctcaact gtgcggcact caacgaatcc ttgctggaat ctgaattgtt
    26821 cggtcacgaa aaaggggcgt ttactggagc cgataaacgc cgggaagggc gctttgttga
    26881 ggcggacggc ggcacgctgt ttctcgatga aattggcgat atctcgccga tgatgcaggt
    26941 gcgtctgctg cgtgcgattc aggagcgcga agttcagcgt gtcggtagca accagactat
    27001 ctcggttgat gtccggctga ttgcggcgac ccatcgcgat cttgccgcag aggtgaatgc
    27061 cgggcgtttt cgccaggatc tctactatcg cctgaatgtg gtggcgattg aagtaccatc
    27121 gctgcggcaa cggcgggagg atatccctct gctggctagc cattttcttc agcgttttgc
    27181 cgagcgtaat cgcaaggcgg taaaaggctt tacgccacag gcgatggatt tgctgatcca
    27241 ctacgactgg ccgggaaaca ttcgtgaact ggaaaacgcg gtggaaaggt cggtggtgct
    27301 gctgaccggg gaatatattt ccgaacgcga gctgccgctg gcgattgcca gtacgccgat
    27361 cccgctgggg caaagtcagg atattcagct gctggtggaa gtcgaaaaag aagtgattct
    27421 ggcggcactg gagaaaacgg gcggcaacaa aaccgaagcc gcccgtcagt tagggatcac
    27481 gcgcaaaacg ctattggcaa aactgtcgcg ttagttctgc tcgcgttcga tagcgcgcca
    27541 gccgatatct ttccggcaga agcagtcgtc ccagtgaata ttggtcatta aggcataagc
    27601 gcgtttctgc gcttctgcca cggtatgacc cagcgcggtg acgcacagta cgcgcccgcc
    27661 gctggtcact acctgctcgt tatccgccag tttcgtgccc gcgtggaaca ctttgccgcc
    27721 tgccacttct tccagcggca ggccgtggat cacgtcaccg gtgcggtaat cacccggata
    27781 tccacccgca gccatcacca cgccgagaga agcgcgttca tcccactctg acgttttctc
    27841 gtccagtttg ccttcacagg ccgccaggca gagctcaacc agatcggact tcatgcgcag
    27901 cataatcggc tgggtttctg gatcgccaaa gcggcagtta aattcgataa ccttcggatt
    27961 gccctgtttg tcgatcatca ggcccgcgta gagaaaaccg gtgtaggtgt tgccttccgc
    28021 cgccatgcct ttcacggttg gccagatgat acgttccatg gtgcgctgat gaacttcatc
    28081 ggttactacc ggcgcggggg agtaagcgcc catcccgccc gtgtttggtc cggtatcttt
    28141 atcgcctacg cgtttgtgat cctggctggt ggccatcggc agcacatgct cgccgtctac
    28201 catcacgata aagctcgctt cttcgccatc gaggaactct tcgataacga tgcgatgacc
    28261 cgcgtcgcca aaagcgttgc ccgccagcat atcgtgaaca gccgcttccg cttcttccag
    28321 cgtcatcgcc acgataacgc ctttcccggc agccagaccg tccgctttaa tgacgattgg
    28381 cgcgcctttc tcacgcagat acgccagcgc aggttctacc tcggtgaagt tctggtattc
    28441 cgccgtaggg atcttatggc gggccaggaa atctttggta aacgcttttg agccttccag
    28501 ttgggccgca cctgcggttg ggccgaagat tttcagcccg gcggcgcgga aggtatcgac
    28561 cacgcctttc accagcggcg cttccgggcc gacgatggtc agatcaatct tttcgttttg
    28621 tgcgaaatcc aacagcgccg ggatatcggt cacgccaata gcaacgtttt gcagcgcggg
    28681 ttccagtgca gtgcctgcat tacccggagc aacaaaaaca gtctcaacca gcggcgactg
    28741 ggccgctttc caggccagcg cgtgctcgcg cccgccgtta ccaatcacta atactttcat
    28801 ctattgctcc attaatggcg gaagtggcgc atgtcggtga agagcatcgc aataccgtgc
    28861 tcgtcggcgg cggcaatcac ttcgtcatca cggatggaac cgccaggctg gattacgcag
    28921 gtcacgcccg cagcggcggc ggcatcaata ccgtcgcgga acgggaagaa cgcgtcagaa
    28981 gccatcgagg aacctttcac ttccaggcct tcatcggcag ctttaatacc ggcgattttc
    29041 gcggagtaca cgcggctcat ctggcccgcg ccaataccga tggtcatatt gtttttggca
    29101 tagacgatag cattggattt cacgaacttc gccactttcc agcagaacag cgcatcgcgc
    29161 agttcctgtt cggtcggctg acgttgggtg acgacgcgca gttcttccgc accgaccatc
    29221 cccaggtcac gatcctgaac cagcagaccg ccgttcacgc gtttgaaatc aagacccgga
    29281 acacgctcgc cccactgacc gcaggtcaga acgcgtacgt tctgcttggc ggcggtgatt
    29341 ttcagggctt cttcgctggc ggaaggcgca ataatcactt cgacaaactg acgagaaatg
    29401 atggcctgtg cggtttccgc atctagctcg cggttaaagg caatgatgcc gccgaatgcg
    29461 gaggttgggt cggttttgta cgcgcgatcg taagcatcaa gaatggaatt gccgatagct
    29521 acaccgcaag ggttggcgtg cttcacaatc acacatgccg gctcggcgaa ctctttcacg
    29581 cactccagcg ccgcatcggt atcggcgatg ttgttataag agagggcttt accctgaacc
    29641 tgggttgcgg tagcaacgga ggcttctttc acattctctt ctatatagaa ggcagcctgc
    29701 tggtggctgt tctcgccgta acgcatatcc tgcttcttaa tgaagttcag gttcagcgtg
    29761 cgtgggaagc gaccagcggc ttctttgctt tcaccgtggt acgccggaac catgctgccg
    29821 aagtagttgg caatcatgct gtcgtaggcg gcagtgtgtt cgaaggcttt gatggcgagg
    29881 tcgaaacggg ttgcaagcgt cagcgatcct tcgttgtcat ccatctcttt aataatggcg
    29941 tcgtagtcgc tgctcttcac cacgattgcg acatctttat ggttcttagc ggcggagcgc
    30001 accatggtcg ggccgccgat atcgatgttc tcaaccgcat cttccagcga gcaaccttca
    30061 cgggccacgg tctgggcgaa cggatacagg ttaacaacca ccatatcgat aggctggatc
    30121 tgatgttctt ccataatggc atcgtcctgg ccgcgacggc ccagaatgcc accatgtact
    30181 ttcggatgca gggtcttcac gcgtccatcc atcatctccg ggaaaccggt gtaatcggaa
    30241 acttcggtta ccggcagacc tttttctgct aacagacggg cagtgccccc tgtagacagc
    30301 agctccacac cgcgtgcgga aagtgcctgg gcgaattcga cgataccggc tttgtcagaa
    30361 acactgagca gagcgcggcg gactggacga cgttgttgca tggtaaatcc cctggatttg
    30421 actattacag agagcgttag ctgaattttt cgcgaaaaac tcagctaacg cccctaacgg
    30481 ggcatcctta tttttcgccc gcattgtaac gaaaacgttt gcgcaacgct cgcgaatttt
    30541 tctctttcaa tggtgatcac aattttgaca gtggttaccg tgggaaaaat acagaaatta
    30601 cattgatgat tgtggataac tctgtgcgta aaaaggtata aagcggggtt ttgctgggga
    30661 atgcagcagt cagtcatttt tctgcaattt ttctattgcg gcctgcggag aactccctat
    30721 aatgcgcctc catcgacacg gcggatgtga atcacttcac acaaacagcc ggttcggttg
    30781 aaga
//
LOCUS       DAZFQD010000054        30514 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.054, whole genome shotgun sequence.
ACCESSION   DAZFQD010000054 DAZFQD010000000
VERSION     DAZFQD010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30514
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.054"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..339
                     /locus_tag="ACHKTW_002421"
     CDS             <1..339
                     /locus_tag="ACHKTW_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaJ viral recombinase family protein"
                     /protein_id="HFO6463432.1"
                     /translation="ACSPDGLCSDGNGLELKCPFTSRDFMKFRLGGFEAIKSAYMAQV
                     QYSMWVMRKDAWYFANYDPRMKREGLHYVVIERNEKYMANFDEMVPEFIEKMDVALAE
                     IGFVFGEQWR"
     gene            336..497
                     /locus_tag="ACHKTW_002422"
     CDS             336..497
                     /locus_tag="ACHKTW_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000682357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1317 family protein"
                     /protein_id="HFO6463433.1"
                     /translation="MKHPHDNIRVGAITFIYSVTKRGWVFPGLSVIRNPLKAQRLAEA
                     INNKRGLYD"
     gene            490..1047
                     /locus_tag="ACHKTW_002423"
     CDS             490..1047
                     /locus_tag="ACHKTW_002423"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000129280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage N-6-adenine-methyltransferase"
                     /protein_id="HFO6463434.1"
                     /translation="MTDFTGSNTPAEHRDSWRTPPEIFAALNAEFVFQLDAAASEKNR
                     LCRLFISQEQNTLTTSWPEAMGYASGYVWLNPPYSNISPFVKKAATENKFSSVGCVML
                     LPADTSVGWFHEAIQTASEVRFITAGRLAFINPLTEKPVSGNNKGSMLIIWHPYPRTH
                     CHFTTVDRGELMAFGSRILARREAA"
     gene            1124..1339
                     /locus_tag="ACHKTW_002424"
     CDS             1124..1339
                     /locus_tag="ACHKTW_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFO6463435.1"
                     /translation="MTPQQENALRSIARQANSEIKKARQQFPDKNVDDICRSVLKKHR
                     ETVTLMGFTPTHLSLAIGMLNGVFKER"
     gene            1438..1656
                     /locus_tag="ACHKTW_002425"
     CDS             1438..1656
                     /locus_tag="ACHKTW_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HFO6463436.1"
                     /translation="MADIFDSASEIEELQRNTAIKMRRQNYQTVSATHCCECGDPIDE
                     RRRLAVQRCRTCASCQEEIELKNKQWGL"
     gene            1704..1937
                     /locus_tag="ACHKTW_002426"
     CDS             1704..1937
                     /locus_tag="ACHKTW_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000490216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFO6463437.1"
                     /translation="MFRIIFPNTWYVDHHGTPCKILRSTHNKVHYIRKGRTCIASMFR
                     FNHDFEPVNKADADRIAEEIETAEHIKKLRDMR"
     gene            1954..2049
                     /locus_tag="ACHKTW_002427"
                     /pseudo
     CDS             1954..2049
                     /locus_tag="ACHKTW_002427"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            2181..3345
                     /gene="intD"
                     /locus_tag="ACHKTW_002428"
                     /pseudo
     CDS             2181..3345
                     /gene="intD"
                     /locus_tag="ACHKTW_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415069.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            complement(3361..3437)
                     /locus_tag="ACHKTW_002429"
     tRNA            complement(3361..3437)
                     /locus_tag="ACHKTW_002429"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3401..3403),aa:Arg,seq:tct)
     gene            3680..4312
                     /gene="fimZ"
                     /locus_tag="ACHKTW_002430"
     CDS             3680..4312
                     /gene="fimZ"
                     /locus_tag="ACHKTW_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria biosynthesis transcriptional regulator
                     FimZ"
                     /protein_id="HFO6463438.1"
                     /translation="MKPTSVIIMDTHPIIRMSIEVLLQKNSELQIVLKTDDYRITIDY
                     LRTRPVDLIIMDIDLPGTDGFTFLKRIKQIQSTVKVLFLSSKSECFYAGRAIQAGANG
                     FVSKCNDQNDIFHAVQMILSGYTFFPSETLNYIKSNKCSTNSSTVTVLSNREVTILRY
                     LVSGLSNKEIADKLLLSNKTVSAHKSNIYGKLGLHSIVELIDYAKLYELI"
     gene            complement(4315..4830)
                     /gene="sfmF"
                     /locus_tag="ACHKTW_002431"
     CDS             complement(4315..4830)
                     /gene="sfmF"
                     /locus_tag="ACHKTW_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria assembly protein"
                     /protein_id="HFO6463439.1"
                     /translation="MRRVLFSCFCGLLWSSSGWAADPLGTININLHGNVVDFSCTVNT
                     ADIDKTVDLGRWPTTQLLNAGDTTALVPFSLRLEGCPPGSVAILFTGTPASDTNLLAL
                     DDPAMAQTVAIELRNSDRSRLALGAASPTEEVDANGNVTLNFFANYRALASGVRPGVA
                     KADAIFMINYN"
     gene            complement(4841..5848)
                     /gene="fimH"
                     /locus_tag="ACHKTW_002432"
     CDS             complement(4841..5848)
                     /gene="fimH"
                     /locus_tag="ACHKTW_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HFO6463440.1"
                     /translation="MKIICRLLLAMACLCLANISWATVCANSTGVAEDEHYDLSNIFN
                     STNNQPGQIVVLPEKSGWVGVSAICPPGTLVNYTYRSYVTNFIVQETIDNYKYMQLND
                     YLLGAMSLVDSVMDIQFPPQNYIRMGTDPNVSQNLPFGVMDSRLIFRLKVIRPFINMV
                     EIPRQVMFTVYVTSTPYDPLVTPVYTISFGGRVEVPQNCELNAGQIVEFDFGDIGASL
                     FSAAGPGNRPAGVMPQTKSIAVKCTNVAAQAYLTMRLEASAVSGQAMVSDNQDLGFIV
                     ADQNDTPITPNDLNSVIPFRLDAAAAANVTLRAWPISITGQKPTEGPFSALGYLRVDY
                     Q"
     gene            complement(5861..8470)
                     /gene="sfmD"
                     /locus_tag="ACHKTW_002433"
     CDS             complement(5861..8470)
                     /gene="sfmD"
                     /locus_tag="ACHKTW_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
                     /protein_id="HFO6463441.1"
                     /translation="MKIPTTTDIPQRYTWCLAGICYSSLAILPSFLSYAESYFNPAFL
                     LENGTSVADLSRFERGNHQPAGVYRVDLWRNDEFIGSQDIVFESTTENTGDKSGGLMS
                     CFNQVLLERIGLNSSAFPELAQQQNNKCINLLKAVPDATINFDFAAMRLNITIPQIAL
                     LSSAHGYIPPEEWDEGIPALLLNYNFTGNRGNGNDSYFFSELSGINIGPWRLRNNGSW
                     NYFRGNGYHSEQWNNIGTWVQRAIIPLKSELVMGDGNTGSDIFDGVGFRGIRLYSSDN
                     MYPDSQQGFAPTVRGIARTAAQLTIRQNGFIIYQSYVSPGAFEITDLHPTSSNGDLDV
                     TIDERDGNQQNYTIPYSTVPILQREGRFKFDLTAGDFRSGNSQQSSPFFFQGTALGGL
                     PQEFTAYGGTQLSANYTAFLLGLGRNLGNWGAVSLDVTHARSQLADDSRHEGDSIRFL
                     YAKSMNTFGTNFQLMGYRYSTQGFYTLDDVAYRRMEGYEYDYDYDGEHRDELIIVNYH
                     NLRFSRKDRLQLNISQSLNDFGSLYISGTHQKYWNTSDSDTWYQVGYTSSWVGISYSL
                     SFSWNESVGIPDNERIVGLNVSVPFNVLTKRRYTRENALDRAYASFNANRNSNGQNSW
                     LAGVGGTLLEGHNLSYHVSQGDTSNNGYTGSATANWQAAYGTLGVGYNYDRDQHDVNW
                     QLSGGVVGHENGITLSQPLGDTNVLIKAPGAGGVRIENQTGILTDWRGYAVMPYATVY
                     RYNRIALDTNTMGNSIDVEKNISSVVPTQGALVRANFDTRIGVRALITVTQGGKPVPF
                     GSLVRENSTGITSMVGDDGQVYLSGAPLSGELLVQWGDGANSRCIAHYVLPKQSLQQA
                     VTVISAVCTHPGS"
     gene            complement(8501..9193)
                     /gene="fimC"
                     /locus_tag="ACHKTW_002434"
     CDS             complement(8501..9193)
                     /gene="fimC"
                     /locus_tag="ACHKTW_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HFO6463442.1"
                     /translation="MMTKIKLLMLIIFYLIISASTHAAGGIALGATRIIYPADAQQTA
                     VWIRNSHTNERFLVNSWIENSSGVKEKSFIITPPLFVSEPKSENTLRIIYTGPPLAAD
                     RESLFWMNVKTIPSVDKNALNGRNVLQLAILSRMKLFLRPIQLQELPAEAPDTLKFSR
                     SGNYINVHNPSPFYVTLVNLQVGSQKLGNAMAAPRVNSQIPLPSGVQGKLKFQTVNDY
                     GSVTPVREVNLN"
     gene            complement(9413..9955)
                     /gene="fimA"
                     /locus_tag="ACHKTW_002435"
                     /pseudo
     CDS             complement(9413..9955)
                     /gene="fimA"
                     /locus_tag="ACHKTW_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308619.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
     gene            10436..11302
                     /gene="folD"
                     /locus_tag="ACHKTW_002436"
     CDS             10436..11302
                     /gene="folD"
                     /locus_tag="ACHKTW_002436"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HFO6463443.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQDE"
     gene            11304..11516
                     /gene="ybcJ"
                     /locus_tag="ACHKTW_002437"
     CDS             11304..11516
                     /gene="ybcJ"
                     /locus_tag="ACHKTW_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HFO6463444.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            11624..12145
                     /gene="ybcI"
                     /locus_tag="ACHKTW_002438"
     CDS             11624..12145
                     /gene="ybcI"
                     /locus_tag="ACHKTW_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFO6463445.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKVIPPRLLFAGIILAMLPDADVLS
                     FKFGVAYGNVFGHRGFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSL
                     LDSVTTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTPYGHQVIISELMWVWLP
                     GMVLMGMLWWRRR"
     gene            complement(12181..13566)
                     /gene="cysS"
                     /locus_tag="ACHKTW_002439"
     CDS             complement(12181..13566)
                     /gene="cysS"
                     /locus_tag="ACHKTW_002439"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFO6463446.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            13740..14234
                     /gene="ppiB"
                     /locus_tag="ACHKTW_002440"
     CDS             13740..14234
                     /gene="ppiB"
                     /locus_tag="ACHKTW_002440"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HFO6463447.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVDGMDVVDKIKGVATGRSGMHQDVPKEDVIIESV
                     TVSE"
     gene            14237..14959
                     /gene="lpxH"
                     /locus_tag="ACHKTW_002441"
     CDS             14237..14959
                     /gene="lpxH"
                     /locus_tag="ACHKTW_002441"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HFO6463448.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQMLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            15077..15586
                     /gene="purE"
                     /locus_tag="ACHKTW_002442"
     CDS             15077..15586
                     /gene="purE"
                     /locus_tag="ACHKTW_002442"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFO6463449.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAAEIFEILNVPYHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNDWRKAQTDEVL
                     ENPDPRGAA"
     gene            15583..16650
                     /gene="purK"
                     /locus_tag="ACHKTW_002443"
     CDS             15583..16650
                     /gene="purK"
                     /locus_tag="ACHKTW_002443"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFO6463450.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWRLRANETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDANYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYASGVIWAQSKFS"
     gene            complement(16787..17680)
                     /gene="ybcF"
                     /locus_tag="ACHKTW_002444"
     CDS             complement(16787..17680)
                     /gene="ybcF"
                     /locus_tag="ACHKTW_002444"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFO6463451.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQRLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKADGSIGPKVTAVSGYVRSRSKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(17677..18492)
                     /gene="ylbF"
                     /locus_tag="ACHKTW_002445"
     CDS             complement(17677..18492)
                     /gene="ylbF"
                     /locus_tag="ACHKTW_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HFO6463452.1"
                     /translation="MTIIHPLLASRSAPNYRQSWRLAGVWRRAINLMTESGELLTLHR
                     QGSGFGPGGWVLRRAQFDALCGGLCGNERPQVVAQGIRLGRFTVKQPQRYCLLRITPP
                     AHPQPLAAAWMQRAEETGLFGPLALAASDPLTAELRQFRHCFQAALNGVKTDWRHWLG
                     KGPGLTPSHDDTLSGMLLAAWYYGALDARSGRPFFACSDNLQLVTTAVSVSYLRYAAQ
                     GYFASPLLHFVHALSCPKRTAVAIDSLLALGHTSGADTLLGFWLGQQLLQGKP"
     gene            complement(18503..19762)
                     /gene="ylbE"
                     /locus_tag="ACHKTW_002446"
     CDS             complement(18503..19762)
                     /gene="ylbE"
                     /locus_tag="ACHKTW_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009029994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HFO6463453.1"
                     /translation="MFTSVAQANAAVIEQIRRARPHWLDVQPASSLISELNEGKTLLH
                     AGPPMRWQEMTGPMKGACVGACLFEGWAKDEAQALAILEQGEVNFIPCHHVNAVGPMG
                     GITSASMPMLVVENVTDGNRAYCNLNEGIGKVMRFGAYGEDVLTRHRWMRDVLMPVLS
                     AALGRMERGIDLTAMMAQGITMGDEFHQRNIASSALLMRALAPQIARLDHDKQHIAEV
                     MDFLSVTDQFFLNLAMAYCKAAMDAGAMIRAGSIVTAMTRNGNMFGIRVSGLGERWFT
                     APVNTPQGLFFTGFSQEQANPDMGDSAITETFGIGGAAMIAAPGVTRFVGAGGMEAAR
                     AVSEEMAEIYLERNMQLQIPGWDFQGACLGLDIRRVVETGITPLINTGIAHKEAGIGQ
                     IGAGTVRAPLACFEQALEALAESMGIG"
     gene            complement(19772..21439)
                     /gene="fdrA"
                     /locus_tag="ACHKTW_002447"
     CDS             complement(19772..21439)
                     /gene="fdrA"
                     /locus_tag="ACHKTW_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HFO6463454.1"
                     /translation="MIHAFIKKGCFQDSVSLMIISRKLSESENVDDVSVMMGTPANKA
                     LLDTTGFWHDDFNNVTPNDICVAIRSEAADAGIAQAIMQQLEEALKQLAQGSGSSQSL
                     TQVRRWDSACQKLSDANLALISVAGEYAAELANQALDRNLNVMMFSDNVTLEDEIQLK
                     TRAREKGLLVMGPDCGTSMIAATPLAFANVMPEGNIGVIGASGTGIQELCSQIALAGE
                     GITHAIGLGGRDLSREVGGISALTALEMLSADEKSEVLAFVSKPPAEAVRLKIVNAMK
                     ATGKPTVALFLGYTPAVARDENVWFASSLDEAARLACLLSRVTARRNAITPASSGFIC
                     GLYTGGTLAAEAAGLLAGHLGVEADDTHHHGMMLDADGHQIIDLGDDFYTVGRPHPMI
                     DPALRNQLIADLGAKPQVRVLLLDVVIGFGATADPAASLVSAWQKACAARSDNQPLYA
                     IATVTGTERDPQCRSQQIATLEDAGIAVVSSLPEATLLAAALIRPLSPATQQHTPSLL
                     ENVAVINIGLRSFALELQSASKPVVHYQWSPVAGGNKKLARLLERLQ"
     gene            21756..22805
                     /gene="allD"
                     /locus_tag="ACHKTW_002448"
     CDS             21756..22805
                     /gene="allD"
                     /locus_tag="ACHKTW_002448"
                     /EC_number="1.1.1.154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate dehydrogenase"
                     /protein_id="HFO6463455.1"
                     /translation="MKISRETLHQLIENKLCQAGLKREHAATVAEVLVYADARGIHSH
                     GAVRVEYYAERISKGGTNREPEFRLEETGPCSAILHADNAAGQVAAKMGMEHAIKTAQ
                     QNGVAVVGISRMGHSGAISYFVQQAARAGFIGISMCQSDPMVVPFGGAEIYYGTNPLA
                     FAAPGEGDEILTFDMATTVQAWGKVLDARSRNMSIPDTWAVDKNGAPTTDPFAVHALL
                     PAAGPKGYGLMMMIDVLSGVLLGLPFGRQVSSMYDDLHAGRNLGQLHIVINPNFFSSS
                     ELFRQHLSQTMRELNAITPAPGFNQVYYPGQDQDIKQRKAAVEGIEIVDDIYQYLISD
                     ALYNTSYETKNPFAQ"
     gene            22827..24062
                     /gene="allC"
                     /locus_tag="ACHKTW_002449"
     CDS             22827..24062
                     /gene="allC"
                     /locus_tag="ACHKTW_002449"
                     /EC_number="3.5.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047652 - allantoate deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA];
                     GO_process: GO:0009442 - allantoin assimilation pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate deiminase"
                     /protein_id="HFO6463456.1"
                     /translation="MITHFRQAIEETLPWLSSFGADPTGGMTRLLYSPEWLETQQQFK
                     KRMAASGLETRFDEVGNLYGRLNGTEYPQEVILSGSHIDTVVNGGNLDGQFGALAAWL
                     AIDWLKTQYGAPLRTVEVVAMAEEEGSRFPYVFWGSKNIFGLANPDDVRNICDAKGNS
                     FVDAMKACGFTLPNAPLTPRQDIKAFVELHIEQGCVLESNGQSIGVVNAIVGQRRYTV
                     TLNGESNHAGTTPMGYRRDTVYAFSRICHQSVEKAKRMGDPLVLTFGKVEPRPNTVNV
                     VPGKTTFTIDCRHTDAAVLRDFTQQLENDMRAICDEMDIGIDIDLWMDEEPVPMNKEL
                     VATLTELCESEKLNYRVMHSGAGHDAQIFAPRVPTCMIFIPSINGISHNPAERTNITD
                     LAEGVKTLALMLYQLAWQK"
     gene            24073..24858
                     /gene="allE"
                     /locus_tag="ACHKTW_002450"
     CDS             24073..24858
                     /gene="allE"
                     /locus_tag="ACHKTW_002450"
                     /EC_number="3.5.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071522 - ureidoglycine aminohydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0010136 - ureide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HFO6463457.1"
                     /translation="MGYLNNVTGYRDDLLANRAIVKHGNFALLTPDGLVKNIIPGFEN
                     CDATILSTPKLGASFVDYLVTLHQNGGNQQGFGGEGIETFLYVISGNITAKAEGKTFA
                     LSEGGYLYCPPGSLMTFVNAQAEDSQIFLYKRRYVPVEGHAPWLVSGNASELERIHYE
                     GMDDVILLDFLPKELGFDMNMHILSFAPGASHGYIETHVQEHGAYILSGQGVYNLDNN
                     WIPVKKGDYIFMGAYSLQAGYGVGRGEAFSYIYSKDCNRDVEI"
     gene            complement(25086..26231)
                     /gene="glxK"
                     /locus_tag="ACHKTW_002451"
     CDS             complement(25086..26231)
                     /gene="glxK"
                     /locus_tag="ACHKTW_002451"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 3-kinase"
                     /protein_id="HFO6463458.1"
                     /translation="MKIVIAPDSFKESLSAEKCCQAIKAGFSTLFPDANYICLPIADG
                     GEGTVDAMVAATGGNIVTLEVCGPMGEKVNAFYGLTGDGKTAVIEMAAASGLMLVAPE
                     KRNPLLASSFGTGELIRHALDNGIRHIILGIGGSATVDGGMGMAQALGVRFLDADGQV
                     LVANGGNLARVASIEMDECDPRLANCHIEVACDVDNPLVGARGAAAVFGPQKGATPEM
                     VEELEQGLQNYARVLQQQTEINVCQMAGGGAAGGMGIAAAVFLNADIKPGIEIVLNAV
                     NLAQAVQGAALVITGEGRIDSQTAGGKAPLGVASVAKQFNVPVIGIAGVLGDGVEVVH
                     QYGIDAVFSILPRLAPLAEVLASGETNLFNSARNIACAIKIGQGIKN"
     gene            complement(26253..27554)
                     /gene="ybbY"
                     /locus_tag="ACHKTW_002452"
     CDS             complement(26253..27554)
                     /gene="ybbY"
                     /locus_tag="ACHKTW_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil/xanthine transporter"
                     /protein_id="HFO6463459.1"
                     /translation="MFNFAVSRESLLSGFQWFFFIFCNTVVVPPTLLSAFQLPQSSLL
                     TLTQYAFLATALACFAQAFCGHRRAIMEGPGGLWWGTILTITLGEASRGTPINDIATS
                     LAVGIALSGVLTMLIGFSGLGHRLARLFTPSVMVLFMLMLGAQLTTIFFKGMLGLPFG
                     IADPNFKIQLPPFALSVAVMCLVLAMIIFLPQRFARYGLLVGTITGWLLWYFCFPSSH
                     SLSGELHWQWFPLGSGGALSPGIILTAVITGLVNISNTNGAIRGTDVFYPQQGAGNTR
                     YRRSFVATGFMTLITVPLAVIPFSPFVSSIGLLTQTGDYTRRSFIYGSVICLLVALVP
                     ALTRLFCSIPLPVSSAVMLVSYLPLLFSALVFSQQITFTARNIYRLALPLFVGIFLMA
                     LPPVYLQDLPLTLRPLLSNGLLVGILLAVLMDNLIPWERIE"
     gene            complement(27611..28972)
                     /gene="allB"
                     /locus_tag="ACHKTW_002453"
     CDS             complement(27611..28972)
                     /gene="allB"
                     /locus_tag="ACHKTW_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004038 - allantoinase activity [Evidence
                     IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase AllB"
                     /protein_id="HFO6463460.1"
                     /translation="MSFDLIIKNGTVILENEARVVDIAVKGGKIAAIGQDLGDAKEVM
                     DASGLVVSPGMVDAHTHISEPGRSHWEGYETGTRAAAKGGITTMIEMPLNQLPATVDR
                     ASIELKFDAAKGKLTIDAAQLGGLVSYNIDRLHELDEVGVVGFKCFVATCGDRGIDND
                     FRDVNDWQFFKGAQKLGELGQPVLVHCENALICDALGEEAKREGRVTAHDYVASRPVF
                     TEVEAIRRVLYLAKVAGCRLHVCHISSPEGVEEVTRARQEGQDVTCESCPHYFVLDTD
                     QFEEIGTLAKCSPPIRDLENQKGMWEKLFNGEIDCLVSDHSPCPPEMKAGNIMEAWGG
                     IAGLQNCMDVMFDEAVQKRGMSLPMFGKLMATNAADIFGLQQKGRIAPGKDADFVFIQ
                     PNSSYVLTNDDLEYRHKVSPYVGRTIGARITKTILRGDVIYDIEQGFPVAPKGQFILK
                     HQQ"
     gene            complement(29032..30486)
                     /gene="ybbW"
                     /locus_tag="ACHKTW_002454"
     CDS             complement(29032..30486)
                     /gene="ybbW"
                     /locus_tag="ACHKTW_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative allantoin permease"
                     /protein_id="HFO6463461.1"
                     /translation="MEHQRKLFQQRGYSEDLLPKTQSQRTWKTFNYFTLWMGSVHNVP
                     NYVMVGGFFILGLSTFSIMLAIILSAFFIAAVMVLNGAAGSKYGVPFAMILRASYGVR
                     GALFPGLLRGGIAAIMWFGLQCYAGSLACLILIGKIWPGFLTLGGDFTLLGLSLPGLI
                     TFLLFWLVNVGIGFGGGKVLNKFTAILNPCIYIVFGGMAIWAISLVGIGPIFDYIPSG
                     IQKAENSGFLFLVVINAVVAVWAAPAVSASDFTQNAHSFREQALGQTLGLVVAYILFA
                     VAGVCIIAGASIHYGADTWNVLDIVQRWDSLFASFFAVLVILMTTISTNATGNIIPAG
                     YQIAAIAPTKLTYKNGVLIASIISLLICPWKLMENQDSIYLFLDIIGGMLGPVIGVMM
                     AHYFVVMRGQINLDELYTAPGDYKYYDNGFNLTAFSVTLVAVILSLGGKFIPFMEPLS
                     RVSWFVGVIVAFAAYALLKKRTTAEKTGEQKTIG"
BASE COUNT         7879 a         7951 c         7426 g         7258 t
ORIGIN      
        1 gcctgctctc ccgatggttt atgcagtgac ggcaacggcc ttgaactgaa atgcccgttt
       61 acctcccggg atttcatgaa gttccggctc ggtggtttcg aggccataaa atcggcttac
      121 atggcccagg tgcagtacag catgtgggtg atgcgaaaag atgcctggta ctttgccaac
      181 tatgacccgc gtatgaagcg tgaaggcctg cattatgtcg tgattgagcg gaatgaaaag
      241 tacatggcga attttgacga gatggtgccg gagttcatcg aaaaaatgga cgtggcactg
      301 gctgaaattg gttttgtatt tggggagcaa tggcgatgaa gcatcctcac gataatatcc
      361 gggtaggcgc gatcactttc atctactccg ttacaaagcg aggctgggta tttcccggcc
      421 tttctgttat cagaaatcca ctgaaagcac agcggctggc tgaggcgata aataataaac
      481 gggggctgta tgactgattt caccggaagc aatactcctg ccgaacatcg cgacagctgg
      541 cgcacaccac cagagatttt tgctgcgctt aatgcagagt tcgtttttca acttgatgct
      601 gccgccagcg aaaaaaaccg actatgtcgg ctttttatct cacaggagca gaacacatta
      661 accacttcat ggcctgaagc aatgggatat gcctctggtt atgtctggtt gaatccacca
      721 tacagcaata tttccccttt tgtgaaaaag gcagccactg aaaacaaatt cagtagtgtg
      781 ggatgtgtaa tgttattgcc tgctgacaca tctgtcggat ggtttcatga agcgatacaa
      841 accgccagtg aggtcagatt catcacggca ggacgactgg catttattaa cccactcact
      901 gagaaacccg tcagtggaaa taataaaggc tcgatgctca ttatctggca cccatacccc
      961 cgtacacact gccactttac gaccgttgat cgtggagagt tgatggcgtt cggctcaagg
     1021 attcttgccc gtcgggaggc tgcatgacaa ccacggaatg catttttctg gcagcgggct
     1081 tcatattctg tgtgcttatg cttgccgaca tgggacttgt tcaatgacac ctcagcagga
     1141 aaacgccctt cgcagcattg cccgtcaggc taattctgaa atcaaaaaag ccagacagca
     1201 gtttccggat aaaaacgtcg atgacatttg ccgtagcgta cttaagaagc accgcgaaac
     1261 ggtaacgctg atgggattca caccgactca tttaagcctg gcgatcggca tgttaaacgg
     1321 cgtctttaag gaacggtgaa catgaaaagc aaaatcatca gggagctaca ggctcctttt
     1381 ttattgttcg cattcatcct caagcgtatt aaccaacaat tcagggatta atggaagatg
     1441 gcagacatct ttgattcagc atcagaaatc gaagaattac agcgcaatac agcaataaaa
     1501 atgcgtcgtc agaactacca gactgtatcc gcaactcatt gttgtgagtg tggcgatccg
     1561 atagatgagc gaagacgcct ggctgttcag cgttgtcgga cttgtgcaag ttgccaggag
     1621 gagatcgaac ttaagaacaa acaatgggga ctgtgatggc ctcaaagcag caaatttcaa
     1681 catcgtccaa ctgaggtgta aaaatgttca gaatcatttt tcctaacacc tggtacgtcg
     1741 accaccacgg cactccctgc aaaatcctgc gttctaccca caacaaagtt cactacatcc
     1801 gaaaaggcag aacatgtatc gccagcatgt tccgctttaa tcatgacttt gaacctgtga
     1861 ataaagctga tgcagatcgg atagcagaag agatcgaaac ggcagaacac attaagaagt
     1921 tacgtgacat gcgttgaaaa agcagaggta accatggaat catacagcct cacactcgat
     1981 gaggcctgtc agtttcttaa gatatccaga ccaaccgcca ccaactggat acgaacaggc
     2041 cgcctacagg caacacgtaa agatccaacc aagccaaaat ctccttacct cacaacacgg
     2101 caagcctgca ttgcggcgct tcagtctccg ctgcatactg tccaggtgag cgcgggtgat
     2161 ggcataacag aggaaagaaa atgtcactct tccgcagaaa tgaaatatgg tatgcctcgt
     2221 attcgctccc gggcgggaaa cgaattaagg aatctcttgg cacaaaggac aagcggcaag
     2281 ctcaggagtt gcacgacaag cgaaaagcag aactctggcg agtagaaaag ctaggggatt
     2341 tacctgatgt cacttttgaa gaggcctgcc taagatggct tgaggaaaaa gctgataaaa
     2401 aatctctcga ttcagataaa agccggattg agttctggct tgaacatttt gagggtataa
     2461 ggcttaaaga tatctcggag gcaaagattt actctgctgt aagcagaatg cataacagaa
     2521 agacgaaaga aatatggaaa cagaaagttc aggccgccat caggaaaggt aaagaaccgc
     2581 ctgtttatga accaaagcca gtatcaactc agacaaaggc aaagcatctt gccatgataa
     2641 aggccattct ccgtgctgca gaacgcgact ggaagtggct ggaaaaagcg cctgtcatca
     2701 agataccagc ggtcagaaac aagcgagtca gatggctgga aaaggaggaa gcaaaacgcc
     2761 ttattgatga gtgccccgaa ccactgaaat ctgtcgtcaa gtttgcgctg gcaactggtc
     2821 tgagaaagtc gaacatcata aatctggaat ggcaacaaat cgacatgcag cgacgagttg
     2881 cctgggtgaa tccagaagag agcaaatcaa accgcgccat tggtgtggcg ctgaacgata
     2941 ccgcctgtaa agtgttgcgt gatcaaatag gcaagcatca caaatgggtg tttgtacata
     3001 ccaaggcggc taagcgagca gatggaacat caacgcctgc ggtcaggaag atgcgcatcg
     3061 acagcaagac atcatggcta tcagcttgtc gtcgtgcagg aattgaagat ttccgtttcc
     3121 atgacctcag acacacctgg gcaagctggc tgattcagtc aggcgtccca ttatcagtgc
     3181 ttcaggaaat gggcggatgg gagttccata gaaatggttc gtaggtatgc tcaccttgcg
     3241 cctaatcatt tgacagagca tgcgaggaaa atagacgaca tttttggtga taatgtccca
     3301 aatatgtccc actctggaat tatggaggat ataaagaagg cgtaactgat tgaattgtaa
     3361 tggcgcgccc tgcaggattc gaacctgcgg cccacgactt agaaggtcgt tgctctatcc
     3421 aactgagcta agggcgcgtt gataccgcaa tgcggtgtaa tcgcgtgaat tatacggtca
     3481 acccttgctg agtcaatggc ttttgatctg gttgctgaac aagtgaacga ccgcgtctga
     3541 ttttctgatt tatttcgcta tagcggcaaa caaacacaca ccgctgcgcg tctgaatcaa
     3601 gaaaacccgt attttcatgt atcaaagtac aatttaccgc cctaacggaa aattgtccgc
     3661 tcctatgaga ctggtaacta tgaaaccaac gtcggtgatc attatggata ctcatcctat
     3721 catcagaatg tctattgaag ttctgttgca aaaaaacagt gaattgcaga ttgtcctgaa
     3781 aacggatgat taccgcataa ccatcgatta tctccgaacc cgtcctgttg atttaatcat
     3841 tatggatata gacttgcccg gaacagacgg ttttaccttc ctgaaaagga tcaaacaaat
     3901 ccagagcaca gtgaaagtgt tatttttatc atcgaaatca gaatgctttt atgctggcag
     3961 agcgatacaa gctggtgcta acggttttgt cagtaaatgc aatgatcaga atgatatttt
     4021 tcatgccgtt cagatgatcc tctccggata cacgtttttt cccagcgaaa cgcttaacta
     4081 tataaaaagc aataaatgta gtacgaatag ttcaacggtc actgtgctat ctaatcgtga
     4141 agtgaccata ttacgttatc tggttagcgg attatctaat aaagaaattg ccgataagtt
     4201 attacttagc aataaaacag ttagtgcgca taaatctaat atttatggca agctaggttt
     4261 gcattcaatt gtagagctta tcgactacgc caaattatac gaattaatat aatattaatt
     4321 ataattgatc ataaatatcg catccgcttt cgccacacct ggccgaacac cgctggctaa
     4381 cgctcgataa ttggcaaaaa agtttagtgt gacattgcca tttgcatcta cttcctcagt
     4441 cgggctcgcc gctcccagtg cgagccggga gcgatcgcta ttacgtaatt cgatggcgac
     4501 ggtttgcgcc attgcgggat catccagagc cagcaggttg gtatcggatg ccggcgttcc
     4561 cgtaaataaa atcgcaactg aacccggagg acatccctcc agccgcaggc taaaagggac
     4621 gagtgccgtg gtatcgccag cgttcagtaa ttgtgtcgta ggccatctgc ctaaatctac
     4681 cgtcttatca atatccgcag tgtttacggt acaggagaaa tcaacaacgt taccgtgcaa
     4741 attgatatta atcgttccca aagggtcagc tgcccatcca ctggaactcc acagtaaccc
     4801 gcagaaacag ctaaagagta ctcttctcat tctccgtacc tcattgataa tcgacgcgta
     4861 aataccccag cgcgctaaac ggcccttcgg tcggtttttg accggtaata ctgataggcc
     4921 aggcgcgaag tgtgacattg gctgccgcag ctgcatccag acggaaagga ataacgctat
     4981 tgagatcgtt aggcgtgatc ggcgtatcgt tctgatcggc gacaataaaa cctaaatcct
     5041 gattgtccga caccatcgcc tgaccagaaa cggcactggc ttccagacgc attgttaaat
     5101 aagcctgcgc agcaacattc gtacatttga ccgcaatgct cttggtttgc ggcatgacac
     5161 cagcaggtcg attacccggc cctgccgcac taaataacga tgcgccgata tcaccaaaat
     5221 caaattcaac aatctgcccg gcatttaatt cgcagttttg cggtacttca acccggccac
     5281 caaaactaat ggtataaaca ggtgtaacca acggatcgta aggcgttgat gtcacataca
     5341 cggtaaacat cacctgtctg gggatctcca ccatgttaat aaagggacga ataaccttta
     5401 aacgaaatat taaacgagaa tccatcaccc cgaatggaag gttttgtgaa acgttaggat
     5461 ctgttcccat ccgaatataa ttttgcgggg ggaactgaat atccatcaca ctatcaacca
     5521 gactcatcgc acctaataga taatcattta attgcatata tttataatta tcgatagttt
     5581 cctgaacaat aaagttggtg acataactac ggtatgtata attcaccagc gtgccgggtg
     5641 gacaaattgc tgacacacct acccagccgg atttttccgg taaaacaaca atctgccctg
     5701 gctggttatt ggtgctatta aagatatttg agagatcata gtgttcatct tctgctacgc
     5761 cagtactatt tgcacaaaca gtagcccagg atatgtttgc cagacacaaa catgccatcg
     5821 ccaataataa tctgcagatt atcttcattg ataatttcct ttatgagcca ggatgtgtgc
     5881 aaactgccga aataacagtg acggcttgct gtaagctttg cttcggcaat acatagtgcg
     5941 caatgcagcg tgagttcgcg ccgtctcccc actgaaccag taattcacca gacaatggcg
     6001 caccacttaa ataaacttgc ccgtcatcac ccaccatact ggttattccg gtactgtttt
     6061 cccgtaccag tgatccaaac ggcaccggtt ttccgccctg ggtaacggta atgagcgccc
     6121 gcacgcctat gcgggtatca aaattggcac gaaccaacgc gccttgcgtc ggcacaacgc
     6181 tgctaatatt tttttcaaca tcgatggaat tccccatcgt attggtatca agcgcgatac
     6241 ggttataccg ataaaccgtg gcatacggca tcaccgcata gccgcgccag tcggttaaaa
     6301 tgccagtttg attttcaatg cgtacaccgc ctgcgccagg cgctttaatc aaaacattgg
     6361 tatcccctaa aggctggctc agcgttatgc cattttcgtg cccgaccaca ccgccagaca
     6421 gctgccagtt aacgtcatgt tgatcgcggt cgtagttata cccgaccccc agcgtaccgt
     6481 aagcggcctg ccagtttgcc gtggcgctgc ccgtataccc attattcgag gtatcaccct
     6541 ggcttacgtg ataactcagg ttgtggcctt ctagtaaggt cccacctaca cctgccagcc
     6601 agctattttg cccgttgctg ttacggttgg cgttaaagga ggcataagcg cggtcgagcg
     6661 cattttcccg ggtgtagcga cgtttggtca gaacattgaa aggcactgaa acattaagtc
     6721 cgacaatacg ttcgttatcg gggatcccta cagattcatt ccacgaaaat gagagcgaat
     6781 aactgatgcc aacccagctg ctggtatacc ccacctgata ccaggtatct gaatccgaag
     6841 tattccagta tttttgatgg gtaccagaga tataaagcga gccaaagtca ttaagtgatt
     6901 gtgaaatatt taactgcaaa cggtctttac ggctaaagcg taaattgtgg taattcacga
     6961 ttatcagttc atcccgatgc tcaccgtcgt aatcgtaatc atattcgtac ccctccattc
     7021 gacgatacgc aacatcatca agggtataaa aaccttgtgt cgaatagcgg taacccatta
     7081 actgaaaatt ggtgccgaag gtgttcatcg atttcgcata gaggaagcga atggaatccc
     7141 cctcatgacg actgtcgtcg gctaactgac tgcgcgcatg ggtcacatcc agcgacactg
     7201 ccccccagtt tccgagattg cgccccagtc ctaataaaaa ggcggtgtaa ttggcagata
     7261 attgcgtccc gccgtaggca gtaaattcct gtggtaaacc gccgagtgcc gtgccctgaa
     7321 aaaagaaagg tgatgattgc tgactattac cgctacgaaa atcgcccgcc gtcaggtcaa
     7381 atttgaaacg cccttcgcgt tgtaaaatcg gcactgttga atacggaatt gtgtaattct
     7441 gctgattgcc atcgcgctcg tcgatggtga catccagatc gccatttgaa gatgtcgggt
     7501 gcaaatctgt aatttcaaaa gcgccggggg aaacatagct ttgatagata ataaaaccat
     7561 tttgccgaat cgttagctgg gccgccgtac gggcaatccc acgtaccgtt ggggcaaacc
     7621 cttgctggct atcaggatac atattatcgg aagagtaaag ccgtatacca cgaaatccaa
     7681 caccatcgaa aatatcactt cctgtattgc cgtctcccat taccagttca cttttcagcg
     7741 gaataatggc gcgctgtacc caggtgccaa tattattcca ctgttctgaa tgatatccat
     7801 ttccgcgaaa ataattccag gaaccattgt tgcgtaaacg ccacgggcca atattaatcc
     7861 cgctgagctc actaaaaaaa tagctatcgt taccattacc tctgttaccg gtgaaattat
     7921 aattcaggag taaagcagga ataccttcat cccactcttc aggcggaatg taaccgtgag
     7981 cgctactcaa caacgctatc tgaggaatag tgatgttcag gcgcatcgct gcaaaatcaa
     8041 agttaattgt ggcatcaggt acagctttca gtaaattgat gcatttattg ttttgctgct
     8101 gggctaactc gggaaatgca ctgctattaa ggccaattcg ttcaaggagt acctggttaa
     8161 aacaggacat taacccacct gatttatcac ctgtattttc tgttgtcgat tcaaatacga
     8221 tatcctgtga accaatgaac tcatcattac gccagagatc cacccgatac actcccgcag
     8281 gttgatgatt gcctctctca aagcgcgata aatcagcaac ggatgtgcca ttctctaata
     8341 aaaatgccgg gttgaaataa ctttccgcat agcttaaaaa ggagggtaaa atggcaagag
     8401 atgaataaca aattccggcc agacaccagg tatacctctg cggaatatcc gtagtagtgg
     8461 gtattttcat aattagctct gatattgtca gatgattcgg ttagtttaag ttcacttctc
     8521 tgaccggggt tactgaacca taatcattaa cggtctgaaa tttcagcttt ccctgcactc
     8581 ctgagggtaa gggaatttgt gaattaactc tgggtgcagc catagcattc cccaactttt
     8641 ggctgcccac ttgtaagtta accagggtga cataaaaagg tgagggatta tgaacattga
     8701 tatagttacc ggatcgcgaa aacttgagtg tgtccggcgc ttctgcgggt aattcttgta
     8761 attgaattgg acggagaaat aatttcatgc gcgataaaat cgccagttgc aaaacattcc
     8821 tgccgttcaa tgcattttta tctaccgaag ggatcgtctt aacattcatc cagaacagag
     8881 actcacgatc tgccgccagc ggtggaccgg tgtaaataat acgcaaggta ttttcgcttt
     8941 tgggttcact aacaaacagt ggcggtgtaa tgatgaatga cttttctttt acaccgctgc
     9001 tgttttcaat ccacgaattg accagaaagc gctcattggt atggctattt ctaatccata
     9061 ccgcagtctg ttgagcatca gcgggataaa taatacgcgt ggcacctaat gcgatccctc
     9121 cggcagcatg ggtgctggcc gaaatgatta aataaaatat aatgagcatc aataacttta
     9181 ttttagtcat catagttaat acgccttgtg tattcctgac ggcaaaaata agaaatataa
     9241 agaaatgatt gctttggttt cttacaacac atcattccct tttcatttaa taaagcggca
     9301 tgatacaaag tgaacatgat ttcgatttac attgccacca taccgcttca gacaaccggt
     9361 acattacttt gttataagac gtgatatata attcaaaaca acgtggtttt gattattcgt
     9421 atctcataat aaaagtcgcg tcagcatttg cttgccctgc tgatgcactt gtaccggtgc
     9481 ctttataacg tgcagaaaaa tgaagaacgt tggtcccggg gatcaagttc tagttggttg
     9541 agaagctatt cccatcaggt ttgagaattg cggatgtatt atcaagtatt tcaataccga
     9601 cacccgttgc tgttgttgta ttggtactgg atgcaatggc caataaatta tcattgattg
     9661 catcagcctg accagaaaat gcaacggcag cattagcggc aacaacagga tcgcagtcgt
     9721 tcaactgaat ggtgaatgga attaatgcag aggtattacc aacagcattg aaaatatcga
     9781 tacgatattg accgagtgtg acaacctggt ctgccgagtc agtattcact gaacaggcag
     9841 cattcaccag ttcgccttca aagtgaattg taccgccatc tacaacggca gcataactac
     9901 ccgtagcggc gaatagtgcg gcagccagcg cagacgaaat aaatcttaat ttcatatata
     9961 ttccttcaat ctcatttatc gactccacat ccgtatataa ccgattactt tatttaagac
    10021 actgatagta gtaaaattcc tttttatcct ctaagaatgt cttaattgaa aatatgcact
    10081 ctattctaaa aaatagagag ccccgttaga tgaatacttc cgcgcaaaat atattcaaca
    10141 caaatataga cctgaagcgg taaattacca ggctgaaaat tctttttata ttgtcaggta
    10201 tttcttaaat tatcttaatc cttaaacaag gaaataaatc agttccagat ttacaacgcc
    10261 atcatggacg aaaaatgaag ctttcaatct cagcgacctc agcgacggtg cgcctaacct
    10321 tcgcaagagg tcgcttcacg cgataaatct gaaacgaaac ctgacagcgc gccccgcttc
    10381 tgacaaaata ggcgcatccc cttcgaccta cgtaacagat ggaatcctct ctctgatggc
    10441 agcaaagatt attgacggta aaacgattgc gcagcaggtg cgctctgaag ttgctcaaaa
    10501 agttcaggcg cgtattgcag ccggactgcg ggcaccagga ctggccgttg tgctggtggg
    10561 tagtaaccct gcatcgcaaa tttatgtcgc aagcaaacgc aaggcttgtg aagaagtcgg
    10621 gttcgtctcc cgctcttatg acctcccgga aaccaccagc gaagcggagc tgttggagct
    10681 tatcgatgcg ctgaatgccg acaacaccat cgatggcatt ctggttcaac tgccgttacc
    10741 ggcgggtatt gataacgtca aagtgctgga acgtattcat ccggacaaag acgtggacgg
    10801 tttccatcct tataacgtcg gtcgtctgtg ccagcgcgcg ccgcgtctgc gtccctgcac
    10861 cccgcgcggt atcgtcacgc tgcttgagcg ttacaacatt gataccttcg gcctcaacgc
    10921 cgtggtgatt ggcgcatcga atatcgtcgg tcgtccaatg agcatggaac tgttgctcgc
    10981 aggttgcacc actaccgtga ctcaccgttt cactaaaaat ctgcgtcacc acgtagaaaa
    11041 tgccgatctg ttgatcgttg ccgtcggcaa gccgggcttt attcctggtg actggatcaa
    11101 agaaggcgca attgtgattg atgtcggcat caaccgtctg gaaaatggca aagttgtggg
    11161 cgacgtcgtg tttgaagacg cggctaaacg cgcctcatac attacgcctg ttcccggcgg
    11221 cgttggcccg atgacggttg ccacgctgat tgaaaacacg ctacaggcgt gcgttgaata
    11281 tcatgatcca caggatgagt aacatggcga cattttcttt aggtaaacat ccgcacgttg
    11341 agctgtgcga cttgctgaaa ctggaaggct ggagcgaaag cggcgcgcag gcgaaaatcg
    11401 cgattgccga aggccaggtg aaagtcgacg gtgcggttga aacgcgcaaa cgctgcaaaa
    11461 tcgtcgccgg tcagacagtg agttttgcag gtcacagcgt acaggttgtt gcctgatccc
    11521 tttcccggcc tgtatcaaca ggccggttct cttttcagac ttttgccctc tacgccccgt
    11581 tcatccatgc tgatagcatc gacattttgc cgaagcgacc atcatgccaa ccgttattac
    11641 gcacgccgct gttcccctat gcattggttt agggttgggt tcgaaagtca tccctccgcg
    11701 tctgttattt gccggaatca tcctggcgat gctgcccgac gccgacgtat tgtcgtttaa
    11761 atttggcgtt gcttacggca atgtttttgg tcatcgcggg tttacccatt cgctggtgtt
    11821 tgcgtttgtt gtcccgctgt tatgtgtgct gattggacgc cgatggttca gggctgggct
    11881 gattcgctgc tggctgtttt taaccgtctc gttgctatcg cacagcttgc tggattcggt
    11941 aaccactggc ggtaagggcg ttggctggct gtggccgtgg tcagatgaac gctttttcgc
    12001 tccctggcag gtgattaaag tcgcgccgtt tgcattgtct cgttacacca cgccgtacgg
    12061 gcatcaggtg ataatttctg aattgatgtg ggtgtggttg ccggggatgg tgcttatggg
    12121 aatgttgtgg tggcgcaggc gataaccgga tgcgaaaact cgcatccggc aacagcgcaa
    12181 ttacttacga cgccaggtgg tcccttgcgg gccatcttcc agcacgatcc ccatctcgtt
    12241 aagacggtca cgcgccgcat ctgccgccgc ccagtctttc gctttacggg catccagacg
    12301 ctgttgaatt aacgcttcaa tctcagccac ttcgctgtcg tctgcctgcg cgccgctttg
    12361 caggaacgct tccggttctt gctccagcag gcccaataca gcggaaagtt tacgcaggtg
    12421 agatgccatt gcattcgccg ctgccatatc ttctgctttc agacggttta cttcacgcgc
    12481 catatcaaac agtacggaat aggcttccgg ggtgttgaaa tcgtcgtcca tcgcttcaat
    12541 aaagcgcgct tcaaacgctt cgccaccggc aggcgcaacg gttttatctg tgccgcgcag
    12601 cgcagtgtag agacgctcca gcgccgcacg cgcctgcttc aggttctctt cgctgtagtt
    12661 caactggctg cgatagtggc ccgacatcag gaagtaacgc acggtttccg cgtcgtagta
    12721 tttcagcaca tcgcgcacgg taaagaagtt acccagcgat ttggacatct tctcgcggtc
    12781 aaccatcacc atccccgagt gcatccagta gttcacatac tggccatcat gggcacaggt
    12841 ggactgcgcg atttcgttct cgtggtgcgg gaacatcagg tctgaaccgc cgccgtggat
    12901 atcaaagtgg ttacccagct gcttgcagtt cattgccgaa cattcaatgt gccagcctgg
    12961 acggcccgcg ccccacggag acggccagct cggttcgccc tctttcgaca tcttccacag
    13021 aacgaagtcc atcgggttgc gtttgtcgtc gaccacgtca acgcgcgcac ctgcctgcag
    13081 ctggtcgaga tcctgacgcg acagcacgcc atatgtcgga tcggtcggga cgtcgaacat
    13141 cacgtcgccg ttgtccgcca cataagcgtg acctttggcg atcagttgtt cagtgagttc
    13201 aataatttct gcgatatggt gcgtcgcgcg cggctccata tccgggcgca gaatgttcaa
    13261 agcatcaaaa tctttgtgca tttcggcgat catgcgatcc accagcgcca caaagctttc
    13321 gccattttca ttggcgcgtt tgatgatttt gtcgtcgata tcggtaatgt tgcgcacata
    13381 cttcagttta tagccgagga aacgcagata gcgcgcaacc acgtcaaaag caacaaaggt
    13441 acgcccgtga ccgatatgac agagatcgta aacggtgatt ccacacacat acatgccgac
    13501 ttccccggcg tgaataggct taaattcctc tttttggcgt gtcagagtat tgaagatttt
    13561 tagcatcgaa gattccgttt agacatgtgt gggtaattga gttgcgtata atacccatat
    13621 ttcccgcgcg aatcagcata cattgcgtga tgatccgatc gtgcggttat gctataacac
    13681 caccctatat atgacccgaa ctgggttgaa gcaccaatca aacggaacag gatgcaaaaa
    13741 tggttacttt ccacaccaat cacggcgata ttgtcatcaa aacttttgac gataaagcac
    13801 ctgaaacagt taaaaacttc ctggactact gccgcgaagg tttttacaac aacaccattt
    13861 tccaccgtgt tatcaacggc tttatgattc agggcggcgg ttttgaaccg ggcatgaaac
    13921 aaaaagccac caaagaaccg atcaaaaacg aagccaacaa cggcctgaaa aatacccgtg
    13981 gtacgctggc aatggcacgt actcaggctc cgcactctgc aactgcacag ttcttcatca
    14041 acgtggttga taacgacttc ctgaacttct ctggcgaaag cctgcaaggt tggggctact
    14101 gcgtgtttgc tgaagtggtt gacggcatgg acgtggtaga caaaatcaaa ggtgttgcaa
    14161 ccggtcgtag cggtatgcac caggacgtgc caaaagaaga cgttatcatt gaaagcgtga
    14221 ccgttagcga gtaatcgtgg cgacactctt tattgcagat cttcatctct gcgtggaaga
    14281 accggcgatc accgccggtt ttctgcgttt tttagcgggg gaagcccgca aggccgacgc
    14341 gctgtatatt cttggcgatc tgtttgaagc atggattggc gacgacgatc ccaacccact
    14401 ccatcgccag atggcggcag cgatcaaagc ggtgtccgat tccggcgttc cctgttattt
    14461 cattcatggc aaccgtgatt ttctgctcgg caaacgcttt gcccgtgaaa gcggcatgac
    14521 attattgccg gaagaaaagg tgctcgaact ttatggtcgc cgggtgttga ttatgcatgg
    14581 cgacacgctg tgcaccgatg acgcgggtta tcaggctttt cgcgccaaag tccacaaacc
    14641 ctggctgcaa atgctattcc tcgccctgcc gttgtttgtg cgcaaacgca ttgccgcgcg
    14701 aatgcgagcg aacagtaaag aagccaacag cagtaaatcg ctggcgatca tggacgttaa
    14761 ccaaaacgcg gtagtcagtg cgatggaaaa acatcaggtg caatggctga tccacggaca
    14821 tacccatcgc ccggcggtac atgaacttat cgccaaccag caacctgctt ttcgcgtggt
    14881 actcggtgcc tggcatacgg aaggttcgat ggtgaaagtc acggcggatg acgttgagct
    14941 gattcatttt ccgttttaaa aaacccgcaa ctttgctgat ttcacagcta cgcaaccgtt
    15001 ttccttgctc tctttccgtg ctattctctg tgccctctaa agccgagagt tgtgcaccac
    15061 aggagtttta agacgcatgt cttcccgcaa taatccggcg cgtgtcgcca tcgtgatggg
    15121 gtccaaaagc gactgggcta ccatgcagtt cgccgccgaa atcttcgaaa tcctgaatgt
    15181 cccgtaccac gttgaagtgg tttctgctca ccgcaccccc gataaactgt tcagcttcgc
    15241 cgaaagcgcc gaagagaacg gttatcaggt gattattgcg ggcgcaggtg gcgcagcgca
    15301 cctgccaggc atgattgccg ccaaaacgct ggtgccggtg ctgggcgtgc cagtacagag
    15361 cgccgcactg agcggtgtcg atagcctcta ctccatcgta caaatgccgc gcggcattcc
    15421 ggtgggtacg ctggcgattg gtaaagctgg cgcggcaaac gcggcattac tggcagcaca
    15481 aattctcgcg actcacgata aagagctaca ccagcgtctg aatgactggc gcaaagccca
    15541 gaccgacgaa gtgctggaaa acccggaccc gcgaggtgcg gcatgaaaca ggtttgcgtc
    15601 ctcggtaacg ggcagttagg ccgtatgctg cgtcaggcag gcgaaccgtt aggcattgct
    15661 gtctggccgg tcgggctgga cgctgaaccg gcggcggtgc cttttcaaca aagcgtgatt
    15721 accgctgaga tcgaacgctg gccggaaacc gcattaaccc gcgagctggc gcgtcatccg
    15781 gcctttgtga accgcgatgt gttcccgatt attgctgacc gtctgactca gaagcagctt
    15841 ttcgataagc tccacctgcc gaccgcgccg tggcagttac ttgccgatcg cagcgagtgg
    15901 cctgcggtgt ttgatcgttt aggtgaactg gcgattgtta agcgtcgcac tggtggctat
    15961 gacggtcgcg gtcaatggcg tttacgcgcc aatgaaaccg aacagttacc ggcagagtgt
    16021 tacggcgaat gtattgtcga gcagggcatt aacttctctg gtgaagtgtc gctggttggc
    16081 gcgcgcggct ttgatggcag caccgtgttt tatccgctga cgcataacct gcatcaggac
    16141 ggtattttgc gcaccagcgt cgcttttccg caggccaacg cacagcagca ggcgcaagcc
    16201 gaagagatgc tgtcggcgat tatgcaggag ctgggctatg tgggcgtgat ggcgatggag
    16261 tgttttgtca ccccgcaagg tctgctgatc aacgaactgg ctccgcgtgt gcataacagc
    16321 ggtcactgga cacaaaacgg tgccagcatc agccagtttg agctgcatct gcgggcgatt
    16381 accgatctgc cgttaccgca accagtagtg aataatccgt cggtgatgat caatctgatt
    16441 ggtagcgatg cgaattatga ctggctgaaa ttgccgctgg tgcatctgca ctggtacgac
    16501 aaagaagtcc gtccggggcg taaagtgggg catctgaatt tgaccgacag cgacacatcg
    16561 cgtctgactg cgacgctgga agccttaatc ccgctgctgc cgccagagta tgccagcggc
    16621 gtgatttggg cgcagagtaa gttcagttaa gcaggtgctc atttgattgc cggatgcggc
    16681 gtaaacgcct tatccggctt accgctccgg tacccattgt aggcctgata agacgcgtca
    16741 agcgtcgcat caggcatggt gcaccgattg ccggatgctc tcatcactac agcgaaatac
    16801 aggtccccgc ttcgcccgcc agcgtctctt caattcgcga taacgcccca atccacgcgg
    16861 gtttactacg gcttctgaca taaccactca ccgccgttac cttcggcccg atcgaaccat
    16921 cggctttggc aaatggcgct aactcatccg gtgtggcatg gcgaatggca cgttgctgcg
    16981 gcgttcccca gttttcatat accgcatcag catcggtgag gatcaccagt ccatcggcat
    17041 taatctgctc ggcgagcaac gcagcggcga gatctttatc aatcaccgct tcactccctg
    17101 ccccatcttc cgtcacaggc acaccgccgc cgccactgca aatcaccaca tgcccctctt
    17161 tgagcaacaa ctcgatggct tcgctgtcga gaattttacg cggttgcgga gacgccacca
    17221 cccggcgcaa gtatttacca tcacgtttca tctgccagcc gtaagctgct tccagtgcct
    17281 cttgttcttc tggctgataa accggaccaa taaatttctc tggctgcaaa aacgccggat
    17341 catcaggcga aacctcaatg cgcgtcagca ccgtcgtaac aggcggcatc tgcggctgtg
    17401 cgctcaacct ctgcgccagc atatagccaa tcatcccctg gctttccgcc accagcacat
    17461 ccagcggata cggttctacc tctttccacg ccagattctg taacgccagc agccccacct
    17521 gcggcccgtt gccgtgaaca atcgccagcc gataagaacg ggccaggcgt gccagcgcgg
    17581 gtacagcact ggcgatattg cgatattgat tttccgccgt cagcgcctca ccgcgctgga
    17641 gtaaggcgtt gcccccaaga gctacaacca gtgttttcat ggttttcctt gtaataattg
    17701 ttggccaagc cagaacccca gcagcgtatc tgcccctgac gtatgcccca gcgccagcag
    17761 cgaatcaatc gcaacagcgg tacgtttcgg gcaactcaga gcatgaacaa agtgcaggag
    17821 tggcgaggcg aaatatcctt gcgcggcata acgtaaataa ctgacgctca ccgctgtggt
    17881 aacgagttga agattgtcgg aacaggcaaa aaacgggcga ccagagcgcg catctaaagc
    17941 gccataatac caggccgcca gcagcattcc gctcaacgtg tcatcatgac ttggcgttaa
    18001 tccgggtcct ttacccagcc agtgccgcca gtcggtctta acgccattga gcgcggcctg
    18061 aaaacagtga cgaaactggc gtaattcagc ggtcagcgga tcgcttgccg ccaacgccag
    18121 tggcccgaaa agcccggttt cctccgcgcg ttgcatccat gcagctgcaa gcggttgagg
    18181 atgcgcaggc ggcgtaatac gcagcaaaca ataacgctgt ggctgtttaa ccgtgaaacg
    18241 cccgaggcga atcccttgag ccacaacctg tggtcgttca ttgccgcata atccaccgca
    18301 taacgcatcg aattgcgcgc ggcgaagcac ccatcctccg gggccgaaac cactaccctg
    18361 acgatgcagc gttaacagtt cgccgctttc cgtcatcagg ttaatcgccc gccgccacac
    18421 tcccgctaac cgccaggact ggcgataatt cggtgcacta cggctggcaa gcagaggatg
    18481 gatgatcgtc attgcgcgtt cctcaaccaa tccccatgct ttcagccagt gcttccagcg
    18541 cctgttcaaa acacgccagc ggtgcccgca cagtgcctgc tccaatctgc ccgatccccg
    18601 cctctttatg ggcgataccg gtattgatga gtggcgtaat gccggtttct accacgcgac
    18661 gaatgtccag tcccaggcac gcgccctgaa aatcccagcc tgggatctgc aactgcatat
    18721 tgcgctcaag gtaaatttcc gccatctctt cagataccgc tctggccgct tccatgccac
    18781 ccgcaccgac aaagcgcgtt acgccaggcg ctgcgatcat tgccgcacct ccgataccaa
    18841 aggtttcggt aatcgcgcta tcgcccatat ccgggttcgc ctgctcctgc gagaagccgg
    18901 tgaaaaacag accttgcgga gtgtttacag gcgcagtaaa ccagcgttcg cccagcccgc
    18961 ttacccgaat cccgaacata ttgccgttgc gggtcattgc cgtgacgatg ctgcctgcgc
    19021 ggatcatcgc gccagcatcc atcgccgcct tgcagtaagc catcgcgagg ttgaggaaga
    19081 actgatcggt cacgctgagg aaatccatca cttcggcgat gtgctgttta tcatgatcga
    19141 ggcgagcaat ttgtggggcc agcgcacgca ttaacagtgc agaggaagca atattgcgtt
    19201 gatggaactc atcgcccatc gtaatgccct gcgccatcat cgccgtgaga tcgataccgc
    19261 gctccatgcg ccccagcgcc gcgcttaata ctggcattaa cacatcgcgc atccagcgat
    19321 ggcgagtcag gacatcttcg ccgtaagcgc caaaacgcat cactttgccg ataccttcgt
    19381 tgaggttgca gtacgcccgg ttgccgtcgg tcacgttctc gaccaccagc atcggcatac
    19441 tggcagaagt aataccgccc attggaccga cggcattcac atggtgacaa ggaatgaagt
    19501 tcacttcccc ctgctccagt attgccagcg cctgcgcttc atctttcgcc cagccttcga
    19561 acagacatgc gcccacgcac gcccctttca tgggtccggt catctcctgc cagcgcattg
    19621 gcggcccggc gtgaagcagt gttttgccct cgtttagttc gctgataagt gaagaagccg
    19681 gttgcacatc cagccagtgt ggacgagcac gacgaatttg ttcgataacc gcagcattgg
    19741 cttgcgccac tgatgtaaac atgggaaccc cttattgcaa acgttctaat aaacgagcca
    19801 gttttttatt gccgccggcg actggcgacc attggtaatg cacaaccggt ttgctggcgc
    19861 tttgtagctc cagcgcaaag ctgcgtaatc cgatgttaat cacggcgacg ttttccagta
    19921 atgacggtgt gtgttgctga gtggcaggcg aaagcggacg aatcagtgcc gccgccagca
    19981 aggtggcttc cggcagcgaa ctcactaccg cgatccctgc atcttccagc gtggcgattt
    20041 gctgcgagcg gcattgcggg tcacgttcag tgcctgtcac cgtggcaatg gcatacagtg
    20101 gttgattgtc tgaacgcgcg gcacatgctt tttgccaggc gcttaccagc gaggcggcag
    20161 gatcggcggt cgcaccgaag ccaatcacga catcaagcag caatacgcgc acttgcggtt
    20221 tagcgccgag atcggcaatt aactgattgc gtaaggctgg gtcgatcatc ggatggggac
    20281 gcccgacggt gtagaaatca tcgccgaggt caataatctg gtgaccatcg gcgtcgagca
    20341 tcatgccgtg atgatgggta tcgtcggctt ccacgccaag gtgcccggca agtaatcccg
    20401 ccgcttcggc agccagcgta ccgccggtat acaaaccgca aataaatccg ctgctggcag
    20461 gcgttatcgc gttacgtcgc gccgtaacgc gtgaaagcag gcaagccagg cgtgcggcct
    20521 catccagcga ggaggcaaac cagacattct cgtcgcgggc caccgccggg gtataaccta
    20581 aaaacagcgc caccgtcggt ttgccggttg ctttcatggc attaacaatt ttcagacgca
    20641 cagcttcggc aggcggtttt gaaacaaatg ccagcacttc gcttttctcg tctgcactga
    20701 gcatttccag cgccgtcagc gcactgatgc cgcccacttc acggctgagg tcgcgcccgc
    20761 caaggccaat cgcgtgagta attccctccc ctgccagcgc aatctgtgag cacagctcct
    20821 gaatcccggt accggaagcg ccaatgacgc caatgttgcc ttccggcatc acgttagcaa
    20881 aagccagcgg cgtggcggca atcatcgacg taccgcagtc cggccccatc accagcaagc
    20941 ctttttctcg cgcgcgggtt ttaagttgga tttcatcttc cagcgtgacg ttatcggaga
    21001 acatcatcac gttgaggttg cgatccagcg cctgattcgc cagctccgcc gcatactcgc
    21061 cagccactga aatcagcgcc agattggcat cggataattt ctgacaggca ctgtcccagc
    21121 gacgcacctg cgtcaaggac tggctgctgc ctgacccctg cgccagttgt tttagcgcct
    21181 cttcaagctg ctgcataatc gcctgcgcga tccccgcatc cgccgcttcg ctacgtattg
    21241 ccacgcaaat atcgttcggc gtgacgttat taaaatcgtc atgccagaaa cctgtggtat
    21301 ctaataacgc tttattggcg ggcgtaccca tcattacgga aacatcatca acattttctg
    21361 attcgctgag ttttcgtgaa ataatcatta aactgaccga atcctgaaaa cacccttttt
    21421 taataaaggc gtggatcata acaactcctt aaaaattgca cccatggcaa ttgcacgagc
    21481 agtgttaatg tggcgattac ggtatcgatg acaacggata aataaggtga tgaatatcac
    21541 acagggatta ttaacaaaag aattcaaata accgccatgc tattacgtgt tattttttaa
    21601 taagcgtggt tgttttaaaa accaggttaa aagcgagcgg attatcaaat aaattctaac
    21661 tatatttttt tgcctgtctg gatcacataa tccagatatt ttccctgtat gttaattgca
    21721 gtcatgcttc acaccgtcgt taaaaaggaa gacagatgaa aatcagtcgg gaaacactcc
    21781 accagctaat tgagaataaa ctctgccagg ctgggttaaa acgtgagcac gctgcaaccg
    21841 tggctgaagt attggtttac gccgatgcca gagggatcca ctctcatggc gcggtgcgcg
    21901 tggaatacta cgcggaacgc atttcaaaag gcggcaccaa ccgcgaaccg gagtttcgtc
    21961 ttgaggaaac cgggccgtgc tcggcaattt tacatgccga caatgccgcc ggacaggtcg
    22021 cggcgaaaat gggtatggaa catgccatca aaaccgccca gcaaaatggc gttgcggtgg
    22081 tcggtatcag ccggatgggt cacagcggcg caatctctta ttttgtgcag caggcagccc
    22141 gcgccggatt cattggcatt tcgatgtgcc agtccgatcc aatggtggtg ccgtttggcg
    22201 gcgcggaaat ttactacggt actaaccccc tggcctttgc cgcgccggga gaaggcgacg
    22261 agatccttac ctttgatatg gcgactaccg tacaggcatg gggaaaagtg ctcgacgccc
    22321 gctcgcgtaa tatgtctatc ccggatacct gggcggtcga taaaaacggt gcaccaacaa
    22381 ccgatccgtt cgcggtacat gctctgctcc ccgccgccgg gccaaaaggg tatggcctga
    22441 tgatgatgat tgacgtcctc tcaggcgtct tactcggctt accgttcggg cgacaggtta
    22501 gttcgatgta tgacgattta cacgcagggc gtaatttggg gcaattacat atagttatta
    22561 atccgaactt tttctcctcc agcgaattat tccgtcaaca tcttagccag accatgcgcg
    22621 aattaaatgc cattaccccc gcgcccggtt ttaaccaagt ttattatccc ggacaggatc
    22681 aggatattaa acaacgcaaa gccgccgtcg aaggcatcga aattgttgat gatatttacc
    22741 agtatttaat ttccgacgcg ctttataaca cgtcatacga aacgaaaaat ccctttgcgc
    22801 aataattatt gagacaggac tttcttatga ttacacattt tcgtcaagcc atagaagaaa
    22861 cgctgccctg gctttcctct tttggcgctg acccaacggg tgggatgacc cgtttacttt
    22921 attcgccgga atggctggaa acccagcagc aatttaaaaa aagaatggca gcaagtgggc
    22981 tggaaacacg tttcgatgaa gtagggaatt tatacggtcg cctgaatggt accgaatatc
    23041 cacaggaagt gattctgagc ggttcgcata tcgataccgt ggttaacggc ggtaacctcg
    23101 acgggcaatt cggcgcgctg gcggcgtggc tggcaattga ctggctgaaa acgcaatacg
    23161 gcgcgccgct acgtacggtc gaagtggtgg cgatggcaga agaagaaggc agccgcttcc
    23221 cgtatgtctt ctggggcagt aaaaatatct ttgggctggc gaatcctgac gacgtgcgga
    23281 atatctgtga tgccaaagga aatagttttg tcgatgcgat gaaggcttgc ggatttactc
    23341 ttccgaacgc cccactaact ccgcgtcagg atattaaagc ctttgttgaa ctgcatattg
    23401 aacagggctg tgtgctggaa agtaatgggc aatcaattgg cgtggtgaat gcaattgtcg
    23461 ggcagcgtcg ttatacggta acgctgaacg gtgaatcaaa ccatgcaggc accacgccga
    23521 tgggttatcg tcgtgataca gtttacgctt tcagtcgcat ttgccatcag tcggtcgaaa
    23581 aagcgaaaag gatgggcgat ccgctggttc tgacctttgg caaagtagag ccgcgcccga
    23641 atacggtaaa tgtggtgccg ggtaaaacca cgttcaccat tgattgtcgt cataccgacg
    23701 ctgccgtgct gcgcgatttc acccaacagt tagaaaacga catgcgggcg atttgcgatg
    23761 aaatggacat tggtattgat atcgatttat ggatggacga agaacccgtg ccgatgaata
    23821 aggagctggt cgccaccctg acagaattgt gtgaaagtga aaaactgaat taccgggtga
    23881 tgcacagtgg tgccgggcac gacgcgcaaa ttttcgcgcc tcgcgtacca acctgcatga
    23941 ttttcattcc cagcatcaac gggatcagcc ataacccggc ggaacgcacc aatattaccg
    24001 accttgccga aggggtcaaa acgttggcac tcatgcttta tcaacttgcc tggcagaaat
    24061 aaggagtcat aaatgggata tttaaataac gtcaccggtt accgcgatga tttactggct
    24121 aaccgtgcga ttgttaaaca cggtaatttc gcactgttaa ccccggatgg tctggtaaaa
    24181 aatattattc cgggctttga aaattgtgac gcgacaatcc tctccacgcc aaagctgggt
    24241 gcctcttttg ttgattatct ggtcacactg catcaaaacg gtggcaacca acagggcttc
    24301 ggtggcgaag gcattgaaac gttcctgtat gtgatctctg gaaatatcac tgccaaagcc
    24361 gaaggcaaaa catttgcctt aagcgaaggt ggctatcttt attgcccacc aggctcctta
    24421 atgacgtttg ttaacgccca ggccgaagac agccaaatct ttttatataa gcgtcgctat
    24481 gttccggtag aaggccatgc accgtggctg gtttctggca atgccagcga actggaacgc
    24541 attcactatg aaggtatgga tgatgtgatc ctgctggatt ttctgcccaa agagttaggt
    24601 tttgatatga acatgcatat cctctctttt gcaccaggtg ccagccacgg ttatatcgaa
    24661 acacatgttc aggaacacgg tgcctatatt ctttccggtc agggggttta taacctcgac
    24721 aataactgga tcccggtgaa aaaaggcgat tacatcttta tgggcgctta ttctttacag
    24781 gctggttatg gtgtagggcg tggtgaagcg ttcagctata tttactcgaa agattgcaac
    24841 cgcgacgtag agatttaagt cataccactc gcctgattat ttttagctat gttggatttt
    24901 gccacggttt ataccgtggc tttttttgta acattgcctg atgcgcttcg cttatcaggc
    24961 ctacatgatc tctgcaatat atagaatttg cgcgattttc aggaaggata tagtactcat
    25021 gccgcatccg gcatgagtac tgcactctta tgcgatataa atcgcatccg ctttaaaggt
    25081 aagggtcagt ttttaattcc ctgacctatt ttaatggcgc aggcaatatt tcgtgcgctg
    25141 ttgaagagat tggtttcacc gctggcgagt acttcggcta aaggtgccag acgtggcaaa
    25201 atgctgaata ccgcatcaat gccgtactgg tgcaccactt ccacgccatc acccaatacg
    25261 ccagcaatcc caatcaccgg tacattaaac tgcttcgcca ccgacgccac acccagcggc
    25321 gctttaccgc ctgccgtttg cgagtcgatg cgcccttccc cggtgatcac cagtgctgca
    25381 ccctgcactg cctgcgcaag attgaccgca ttcaacacaa tctcaatacc cggtttaata
    25441 tccgcattga gaaataccgc cgccgcaata cccataccgc ccgcagcgcc gccgcccgcc
    25501 atctggcaga cattaatttc cgtttgctgt tgtaaaacac gggcgtaatt ttgcagcccc
    25561 tgttccagtt cttcgaccat ttccggcgtt gccccttttt gcgggccaaa caccgccgcc
    25621 gcgccgcgtg cccctaccag cgggttatca acgtcacatg ctacttcaat atggcaattc
    25681 gccagacgcg gatcgcattc atccatctca atgcttgcca cgcgcgctaa attaccgccg
    25741 ttagccacca gcacctgacc gtcggcatca aggaaacgca caccgagcgc ctgcgccata
    25801 cccataccac cgtcgaccgt cgcactgccg ccaatgccga gaataatatg gcgaatgccg
    25861 ttatccagcg catgacgaat taactccccc gtaccaaaac tggaggccag caacggatta
    25921 cgcttttcag gcgcgaccag catcaggcca cttgctgccg ccatctcaat caccgccgtt
    25981 ttcccgtcgc cggtaaggcc ataaaaagca ttcacttttt cgcccatcgg cccgcagact
    26041 tcaagcgtca cgatgttgcc gcccgtcgcg gcgaccatcg catccaccgt tccttcgccg
    26101 ccatccgcta tcggcaaaca gatatagttc gcatcgggaa agagggtcga aaacccggct
    26161 ttaattgcct gacaacattt ttctgcactt aagctctctt taaaagagtc tggcgcaatg
    26221 acaatcttca ttatcaatcc tttttcaaca aattattcga tacgttccca cggaataagg
    26281 ttatccataa gaacagccag taaaatcccg accaataagc cgttactgag cagaggacga
    26341 agcgttaatg gaaggtcttg cagatacaca ggcggtaatg ccattaaaaa tatgccgaca
    26401 aataacggca atgcgagtcg ataaatattg cgagcggtaa aggttatttg ctggctaaac
    26461 accagcgcgg aaaagagtaa aggcagataa gaaaccagca tgaccgcgct actcacgggt
    26521 aaagggatac tgcaaaacag tcgtgtgagt gccggaacca gcgccaccag caggcaaata
    26581 acgctgccat aaataaacga acgccgcgtg taatcgccag tctgggttaa taaaccaatg
    26641 gatgaaacaa acggtgaaaa tggaattacc gccagcggta cggttatcag cgtcataaat
    26701 ccggtcgcca caaagctacg acgataacgc gtattccccg cgccctgctg cggataaaaa
    26761 acatccgtgc cccgaatcgc accgttggta ttgctgatat tcaccagacc tgtaatcacc
    26821 gccgtcagaa taattcccgg cgacaaagca ccgccactgc cgagcgggaa ccactgccag
    26881 tgcaactcac cggagagcga atgcgaagaa ggaaagcaaa agtaccacaa caaccagccg
    26941 gttatcgtac cgaccagcag gccataacgg gcaaaacgtt gcggcagaaa gataatcatc
    27001 gccagtacca ggcacatcac cgccaccgag agcgcgaacg gtggtaactg aattttaaaa
    27061 tttgggtcgg ctatgccaaa cggcagcccg agcatacctt tgaaaaagat agtggtcagc
    27121 tgcgcgccca gcatcaacat aaacaagacc atcaccgacg gcgtaaataa ccgtgccagg
    27181 cgatggccta atccgctaaa accaatcaac atcgtcagca cgccggagag tgcaatcccc
    27241 accgccagac tggtagcgat atcgttgatc ggtgtcccgc gcgatgcttc gccaagggtg
    27301 atagtaagga tggttcccca ccacaggcca cccggccctt ccataatagc gcgacgatga
    27361 ccgcagaacg cctgagcgaa gcaggccagt gcggtagcaa gaaaagcata ttgcgtgagc
    27421 gtaagcaggc tactttgcgg caactgaaaa gcagaaagta gcgtaggagg aaccacaacc
    27481 gtgttgcaaa aaataaagaa aaaccactga aatcctgata acaggctttc gcggctgact
    27541 gcaaaattga acataaacac accttaaccg gagaatgccc gccgcaagga cgggcattgc
    27601 aggggccaga ttactgctga tgtttaagga taaattgacc tttcggcgca acagggaagc
    27661 cctgttcaat gtcgtaaatc acatcaccac gtaagatggt tttcgtgata cgcgcgccaa
    27721 tggtacggcc aacatacggg ctgactttgt ggcgatattc cagatcgtca ttggtaagaa
    27781 catagctgct attcggctga atgaagacga agtcggcatc ttttcctggg gcgatacggc
    27841 ctttttgctg cagaccgaaa atatctgctg cgttagtcgc cattaatttg ccgaacattg
    27901 gcagagacat tccgcgtttc tgtaccgctt catcgaacat cacgtccata cagttttgca
    27961 gaccggcaat accgccccat gcttccatga tgttgccggc tttcatttcc ggagggcatg
    28021 gtgagtggtc ggaaaccagg cagtcgattt caccgttaaa cagtttttcc cacatgcctt
    28081 tctggttttc cagatcgcgg atcggcggtg aacacttcgc cagagtgccg atttcttcga
    28141 actgatcggt atccagtaca aagtaatgcg ggcaggattc acaagtaacg tcctgacctt
    28201 cctgacgtgc acgagtcact tcttcaacgc cttccgggct gctgatatgg caaacgtgca
    28261 gacggcaacc ggcaactttc gccagataca gcacgcggcg aatcgcttcc acttcggtaa
    28321 ataccggacg cgaagccaca tagtcatggg cagttacgcg accttcacgt ttcgcttctt
    28381 cgcccagtgc gtcacagatc agcgcgtttt cgcagtgcac cagcaccggc tgccccagtt
    28441 cgcccagctt ctgcgcacct ttgaagaact gccagtcgtt tacatcacgg aagtcgttgt
    28501 cgataccgcg atcgccacag gtcgcaacga agcatttgaa gccgacaacg cccacttcat
    28561 ccagctcatg cagacggtcg atgttgtaag acaccaggcc accgagttgt gccgcatcga
    28621 tagtcagctt gcctttagcg gcatcgaact tcagttcaat tgaagcgcgg tcaaccgttg
    28681 caggcagctg gttgagcggc atttcgatca tggtggtgat accacctttt gccgctgcgc
    28741 gagtaccggt ttcataacct tcccagtggc tacgacccgg ttcagaaata tgggtgtgcg
    28801 catcaaccat gcccggcgaa accaccagac cagacgcatc cataacttct tttgcatcgc
    28861 ccagatcctg accgatagca gcaattttcc cgcctttaac ggcgatatct acaacgcgag
    28921 cttcgttttc taaaataacg gtgccgtttt taatgattaa atcaaaagac ataacaaact
    28981 ccttgtaaaa tacagattgg tttttaactc aatgttcaat atcgggatta attaacctat
    29041 ggttttttgc tctcctgttt tttctgctgt tgtacgtttc tttaataagg cgtaggccgc
    29101 aaaggcgacg atgacgccga caaaccatga aacacgcgat aacggttcca taaagggaat
    29161 aaacttaccg ccaagagata aaataacggc caccagagtt actgaaaacg cggtgaggtt
    29221 aaaaccgtta tcgtaatatt tataatcgcc aggtgcggtg tacagttcat caagattaat
    29281 ttgcccacgc atcaccacaa aataatgtgc catcatgaca ccaattaccg gacccagcat
    29341 tccgccgatg atatcgagga acagataaat actgtcctga ttttccatta atttccacgg
    29401 gcagatcagc aggctgataa tactggcaat cagtacgccg tttttatagg tcagttttgt
    29461 cggtgcaatg gcagcaatct gataaccggc cggaataata ttaccggtgg cgttagtgga
    29521 aatagtcgtc atcagaataa ccagtaccgc aaagaacgag gcaaacaggc tgtcccaacg
    29581 ctgaacaata tccagcacgt tccaggtgtc agcgccgtag tgaatactgg ctccggcaat
    29641 aatacatacc ccagcgaccg caaacagaat ataggccaca actaaaccca gcgtttgccc
    29701 cagcgcttgc tcacgaaacg agtgggcgtt ttgcgtaaag tcggatgcgc tcaccgccgg
    29761 tgccgcccag accgcaacta ccgcgttaat caccaccagg aacaagaatc cactgttttc
    29821 tgctttctga ataccgctcg gaatgtagtc aaagattgga ccgatcccga ccagtgaaat
    29881 cgcccaaatc gccatgccgc cgaaaacgat atagatgcac gggttaagaa tggcagtgaa
    29941 tttatttaaa actttgccac cgccaaaacc aataccaacg ttaaccagcc agaagagtaa
    30001 gaaagtaatt aagcccggta gcgaaaggcc taacaaggta aaatcaccac cgagagttaa
    30061 aaatcccggc cagattttgc caatcaggat caagcaggcc agtgatccgg cgtaacattg
    30121 caggccaaac cacataatgg cagcaatacc acctcttaat aatccgggaa acagtgcgcc
    30181 acgtacgccg taagaagcac gcaggatcat ggcaaacggt acgccgtatt tactgcccgc
    30241 agcaccgttt aataccatta ccgcggcaat gaaaaaggcg ctgaggataa ttgccagcat
    30301 aatactaaag gtagacaagc cgagaataaa aaagccgccg accatcacat aattgggaac
    30361 gttatgaacc gaacccatcc ataaggtaaa atagttaaat gttttccagg tccgctggct
    30421 ttgcgttttc ggcaataaat cttcgctata gccgcgttgc tggaatagct ttctctgatg
    30481 ttccataaat ccctcttaat ttcacgtatc gaat
//
LOCUS       DAZFQD010000055        30019 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.055, whole genome shotgun sequence.
ACCESSION   DAZFQD010000055 DAZFQD010000000
VERSION     DAZFQD010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30019
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.055"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(29..1483)
                     /gene="xylB"
                     /locus_tag="ACHKTW_002455"
     CDS             complement(29..1483)
                     /gene="xylB"
                     /locus_tag="ACHKTW_002455"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HFO6463462.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVASQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMPALYEGSEITGALLPEVAK
                     AWGMATVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWVAKLTGLCNVPALIAAAQQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGDVGPALGAARLAQIAANPEKSLIELLPQLP
                     LEQSHLPDAQRYAAYQPRRETFRRLYQQLLPLMA"
     gene            complement(1555..2877)
                     /gene="xylA"
                     /locus_tag="ACHKTW_002456"
     CDS             complement(1555..2877)
                     /gene="xylA"
                     /locus_tag="ACHKTW_002456"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HFO6463463.1"
                     /translation="MQAYFDQLDRVRYEGSKSSNPLAFRHYNPDELVLGKRMEEHLRF
                     AACYWHTFCWNGADMFGVGAFNRPWQQPGEALALAKRKADVAFEFFHKLHVPFYCFHD
                     VDVSPEGASLKEYINNFAQMVDVLAGKQEESGVKLLWGTANCFTNPRYGAGAATNPDP
                     EVFSWAATQVVTAMEATHKLGGENYVLWGGREGYETLLNTDLRQEREQLGRFMQMVVE
                     HKHKIGFQGTLLIEPKPQEPTKHQYDYDAATVYGFLKQFGLEKEIKLNIEANHATLAG
                     HSFHHEIATAIALGLFGSVDANRGDAQLGWDTDQFPNSVEENALVMYEILKAGGFTTG
                     GLNFDAKVRRQSTDKYDLFYGHIGAMDTMALALKIAARMIEDGELDKRIAQRYSGWNS
                     ELGQQILKGQMSLADLAKYAQEHNLSPVHQSGRQEQLENLVNHYLFDK"
     gene            3243..4235
                     /gene="xylF"
                     /locus_tag="ACHKTW_002457"
     CDS             3243..4235
                     /gene="xylF"
                     /locus_tag="ACHKTW_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015753 - D-xylose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO6463464.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNEETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKEA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     AKLFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVAS
                     NDATAGGAIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAE
                     IAVELGNGQEPKADTTLNNGLKDVPSRLLTPIDVNKNNIKDTVVKDGFHKESEL"
     gene            4313..5854
                     /gene="xylG"
                     /locus_tag="ACHKTW_002458"
     CDS             4313..5854
                     /gene="xylG"
                     /locus_tag="ACHKTW_002458"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015614 - ABC-type D-xylose transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015753 - D-xylose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HFO6463465.1"
                     /translation="MPYLLEMKNITKTFGSVKAIDNVCLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRCQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVGRELTALYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     IAQGIAMVPEDRKRDGIVPVMAVGKNITLAALNKFTGGISQLDDAAEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            5832..7013
                     /gene="xylH"
                     /locus_tag="ACHKTW_002459"
     CDS             5832..7013
                     /gene="xylH"
                     /locus_tag="ACHKTW_002459"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HFO6463466.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            7091..8269
                     /gene="xylR"
                     /locus_tag="ACHKTW_002460"
     CDS             7091..8269
                     /gene="xylR"
                     /locus_tag="ACHKTW_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312479.1"
                     /note="D-xylose enhances binding of XylR to the xyl
                     promoter and activates transcription; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose utilization transcriptional activator
                     XylR"
                     /protein_id="HFO6463467.1"
                     /translation="MFTKRHRITLLFNANKAYDRQVVEGVGEYLQASQSEWDIFIEED
                     FRARIDKIKDWLGDGVIADFDDKQIEQALADVDVPIVGVGGSYHLAESYPPVHYIATD
                     NYALVESAFLHLKEKGVNRFAFYGLPESSGKRWATEREYAFRQLVAEEKYRGVVYQGL
                     ETAPENWQHAQNRLADWLQTLPPQTGIIAVTDARARHILQVCEHLHIPVPEKLCVIGI
                     DNEELTRYLSRVALSSVAQGARQMGYQAAKLLHRLLDKEEMPLQRILVPPVRVIERRS
                     TDYRSLTDPAVIQAMHYIRNHACKGIKVDQVLDAVGISRSNLEKRFKEEVGETIHAMI
                     HAEKLEKARSLLISTTLSINEISQMCGYPSLQYFYSVFKKAYDTTPKEYRDVNSEVML
                     "
     gene            complement(8377..9201)
                     /gene="bax"
                     /locus_tag="ACHKTW_002461"
     CDS             complement(8377..9201)
                     /gene="bax"
                     /locus_tag="ACHKTW_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HFO6463468.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTPRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWSPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            9521..11551
                     /gene="malS"
                     /locus_tag="ACHKTW_002462"
     CDS             9521..11551
                     /gene="malS"
                     /locus_tag="ACHKTW_002462"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004556 - alpha-amylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HFO6463469.1"
                     /translation="MKLAACFLTLLPGFAVAASWTSPGFPAFSEQGTGTFVSHAQLPK
                     GTRPLTLNFDQQCWQPADAIKLNQMLSLQPCSNTPPQWRLFRDGKYTLQIDTRSGTPT
                     LMISIQNAAEPVANLVRECPKWDGLPLTLDVSATFPEGAAVRDYYSQQIAIVKNGQIT
                     LQPAATSNGLLLLERAETDAPAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMA
                     EIGTFHGGDLRGLTNKLDYLQQLGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYY
                     TQDWTNLDANMGNEADLRTLVDSAHQRGIRILFDVVMNHTGYATLADMQEYQFGALYL
                     SGDEVKKTLGERWSDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNP
                     GFDDLTMSLAFLPDIKTESTTASGLPVFYKNKTDTHAKVIEGFTPRDYLTHWLSQWVR
                     DYGIDGFRVDTAKHVELPAWQQLKTEASAALREWKKANPDKALDDKPFWMTGEAWGHG
                     VMQSDYYRHGFDAMINFDYQEQAAKAVDCLAQMDTTWQQMAEKLQGFNVLSYLSSHDT
                     RLFREGGDKAAELLLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDMNWQDVSGKSA
                     ANVAHWQKISQFRARHPAIGAGKQTTLSLKQGYGFVREHGDDKVLVIWAGQQ"
     gene            11729..12982
                     /gene="avtA"
                     /locus_tag="ACHKTW_002463"
     CDS             11729..12982
                     /gene="avtA"
                     /locus_tag="ACHKTW_002463"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HFO6463470.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLREKLGWDIEAQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEARPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPEDRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
     gene            complement(13133..13606)
                     /gene="ysaA"
                     /locus_tag="ACHKTW_002464"
     CDS             complement(13133..13606)
                     /gene="ysaA"
                     /locus_tag="ACHKTW_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFO6463471.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQDNQDCAALSPDEFISRI
                     RVIKDHSWTTAVACHQCEDAPCANVCPVDAISREHGHIFVEQTRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRETGPACVEACPTKALQCMDVEKVQRHRLRQQPV"
     gene            complement(13708..14556)
                     /gene="yiaJ"
                     /locus_tag="ACHKTW_002465"
     CDS             complement(13708..14556)
                     /gene="yiaJ"
                     /locus_tag="ACHKTW_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator YiaJ"
                     /protein_id="HFO6463472.1"
                     /translation="MGKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCP
                     LAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIIH
                     IAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMG
                     KIYMAFGHPDYVKSYWENHQHEIQPLTRNTITELPAMFDELAHIRESGAAMDREENEL
                     GVSCIAVPVFNIHGRVPYAVSISLSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRD
                     DQGAIT"
     gene            14757..15755
                     /gene="yiaK"
                     /locus_tag="ACHKTW_002466"
     CDS             14757..15755
                     /gene="yiaK"
                     /locus_tag="ACHKTW_002466"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047559 - 3-dehydro-L-gulonate
                     2-dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HFO6463473.1"
                     /translation="MKVTFEQLKAAFNRVLISRGVDSETADACAEMFARTTESGVYSH
                     GVNRFPRFIQQLENGDIIPDAQPKRITSLGAIEQWDAQRSIGNLTAKKMMDRAIELAA
                     DHGIGLVALRNANHWMRGGSYGWQAAEKGYIGICWTNSIAVMPPWGAKECRIGTNPLI
                     VAIPSTPITMVDMSMSMFSYGMLEVNRLAGRQLPVDGGFDDEGNLTKEPGVIEKNRRI
                     LPMGYWKGSGMSIVLDMIATLLSDGASVAEVTQDNSDEYGISQIFIAIEVDKLIDGPT
                     RDAKLQRIMDYVTTAERADENQAIRLPGHEFTTLLAENRRNGITVDDSVWAKIQAL"
     gene            15767..16234
                     /gene="yiaL"
                     /locus_tag="ACHKTW_002467"
     CDS             15767..16234
                     /gene="yiaL"
                     /locus_tag="ACHKTW_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HFO6463474.1"
                     /translation="MIFGHIAQPNPCRLPAAIEKALDFLRATDFNALEPGVVEIDGKN
                     IYAQIIDLTTREAVENRPEVHRRYIDIQFLAWGEEKIGIAIDTGNNKVSESLLEQRDI
                     IFYHDSEHESFIEMIPGSYAIFFPQDVHRPGCILQTASEIRKIVVKVALTALN"
     gene            16352..16825
                     /gene="yiaM"
                     /locus_tag="ACHKTW_002468"
     CDS             16352..16825
                     /gene="yiaM"
                     /locus_tag="ACHKTW_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter small
                     permease YiaM"
                     /protein_id="HFO6463475.1"
                     /translation="MKKILEAILAINLAVLSCIVFINIILRYGFQTSILSVDELSRYL
                     FVWLTFIGAIVAFMDNAHVQVTFLVEKLSPAWQRRVALVTHSLILFICGALAWGATLK
                     TIQDWSDYSPILGLPIGLMYAACLPTSLVIAFFELRHLYQLITRSNSLTSPPQGA"
     gene            16828..18105
                     /gene="yiaN"
                     /locus_tag="ACHKTW_002469"
     CDS             16828..18105
                     /gene="yiaN"
                     /locus_tag="ACHKTW_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter large
                     permease YiaN"
                     /protein_id="HFO6463476.1"
                     /translation="MAVLIFLGCLLGGIAIGLPIAWALLLCGAALMFWLDMFDVQIMA
                     QTLVNGADSFSLLAIPFFVLAGEIMNAGGLSKRIVDLPMKLVGHKPGGLGYVGVLAAM
                     IMASLSGSAVADTAAVAALLVPMMRSANYPVNRAAGLIASGGIIAPIIPPSIPFIIFG
                     VSSGLSISKLFMAGIAPGMMMGATLMLTWWWQASRLNLPRQQKATMQEIWHSFVSGIW
                     ALFLPVIIIGGFRSGLFTPTEAGAVAAFYALFVATVIYREMTFATLWHVLIGAAKTTS
                     VVMFLVASAQVSAWLITIAELPMMVSDLLQPLVDSPRLLFIVIMVAILIVGMVMDLTP
                     TVLILTPVLMPLVKEAGIDPIYFGVMFIINCSIGLITPPIGNVLNVISGVAKLKFDDA
                     VRGVFPYVLVLYSLLVVFVFIPDLIILPLKWIN"
     gene            18118..19104
                     /gene="yiaO"
                     /locus_tag="ACHKTW_002470"
     CDS             18118..19104
                     /gene="yiaO"
                     /locus_tag="ACHKTW_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter
                     substrate-binding protein YiaO"
                     /protein_id="HFO6463477.1"
                     /translation="MKLRSVTYALFIAGLAAFSTSSLAAQSLRFGYETSQTDSQHIAA
                     KKFNDLLQERTKGELKLKLFPDSTLGNAQAMISGVRGGTIDMEMSGSNNFAGLSPVMN
                     LLDVPFLFRDTAHAHKTLDGKVGDDLKASLEGKGLKVLAYWENGWRDVTNSRAPVKTP
                     ADLKGLKIRTNNSPMNIAAFKVFGANPIPMPFAEVYTGLETRTIDAQEHPINVVWSAK
                     FFEVQKYLSLTHHAYSPLLVVINKAKFDGLTPEFQQALISSAQEAGNYQRKLVAEDQQ
                     KIIDGMKEAGVEVITDLDRKAFSDALGTQVRDMFVKDVPQGADLLKAVDEVQ"
     gene            19108..20604
                     /gene="lyxK"
                     /locus_tag="ACHKTW_002471"
     CDS             19108..20604
                     /gene="lyxK"
                     /locus_tag="ACHKTW_002471"
                     /EC_number="2.7.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-xylulokinase"
                     /protein_id="HFO6463478.1"
                     /translation="MTQYWLGLDCGGSWLKAGLYDREGREAGVQRLPLCALSPQPGWA
                     ERDMAELWQCCMAVIRELLTHSGVSGEQIVGIGISAQGKGLFLLDKNNKPLGNAILSS
                     DRRAMEIVRRWQEDGIPEKLYPLTRQTLWTGHPVSLLRWLKEHEPERYAQIGCVMMTH
                     DYLRWCLTGVKGCEESNISESNLYNMSLGEYDPCLTDWLGIAEINHALPPVVGSAEIC
                     GEITAQTAVLTGLKAGTPVVGGLFDVVSTALCAGIEDEFTLNAVMGTWAVTSGITRGL
                     RDGEAHPYVYGRYVNDGEFIVHEASPTSSGNLEWFTAQWGEISFDEINQAVASLPKAG
                     GDLFFLPFLYGSNAGLEMTSGFYGMQAIHTRAHLLQAIYEGVVFSHMTHLNRMRERFT
                     DVHTLRVTGGPAHSDVWMQMLADVSGLRIELPQVEETGCFGAALAARVGTGVYHNFSE
                     AQRDLRHPVRTLLPDMTAHQLYQKKYQRYQHLIAALQGFHARIKEHTL"
     gene            20601..21263
                     /gene="ulaD"
                     /locus_tag="ACHKTW_002472"
     CDS             20601..21263
                     /gene="ulaD"
                     /locus_tag="ACHKTW_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFO6463479.1"
                     /translation="MSRPLLQLALDHSSLEAAQRDVTLLKDSVDIVEAGIILCLNEGL
                     GAVKALREQCPDKIIVADWKVADAGETLAQQAFGAGANWMTIICAAPLATVEKGHAMA
                     QRCGGEIQIELFGNWTLDDARDWHRIGVRQAIYHRGRDAQASGQQWGEADLARMKALS
                     DIGLELSITGGITPADLPLFKDIRVKAFIAGRALAGAANPAQVAGDFHAQIDAIWGGA
                     RA"
     gene            21256..22116
                     /gene="sgbU"
                     /locus_tag="ACHKTW_002473"
     CDS             21256..22116
                     /gene="sgbU"
                     /locus_tag="ACHKTW_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HFO6463480.1"
                     /translation="MRNHPLGIYEKALAKDLSWPERLVLAKSCGFDFVEMSVDETDER
                     LSRLDWSAAQRTSLVAAMIETGVGIPSMCLSAHRRFPFGSRDEAVRERAREIMSKAIR
                     LARDLGIRTIQLAGYDVYYEDHDEGTRQRFAEGLAWAVEQAAASQVMLAVEIMDTAFM
                     NSISKWKKWDEMLASPWFTVYPDVGNLSAWGNDVPAELKLGIDRIAAIHLKDTQPVTG
                     QSPGQFRDVPFGEGCVDFVGIFKTLHELNYRGSFLIEMWTEKAKEPVLEIIQARRWIE
                     ARMQEAGFIC"
     gene            22110..22805
                     /gene="araD"
                     /locus_tag="ACHKTW_002474"
     CDS             22110..22805
                     /gene="araD"
                     /locus_tag="ACHKTW_002474"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFO6463481.1"
                     /translation="MLEQLKADVLAANLALPAHHLVTFTWGNVSAVDDTRQWMVIKPS
                     GVEYDVMTADDMVVVEIASGKVVEGSKKPSSDTPTYLALYRRYAEIGGIVHTHSRHAT
                     IWSQAGLDLPAWGTTHADYFYGAIPCTRLMTAEEINGEYEYQTGEVIIETFEERGRSP
                     AQIPAVLVHSHGPFAWGKNAADAVHNAVVLEECAYMGLFSRQLAPQLPAMQNELLDKH
                     YLRKHGDNAYYGQ"
     gene            complement(22837..22938)
                     /locus_tag="ACHKTW_002475"
                     /pseudo
     CDS             complement(22837..22938)
                     /locus_tag="ACHKTW_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312484.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
     gene            22942..23205
                     /locus_tag="ACHKTW_002476"
                     /pseudo
     CDS             22942..23205
                     /locus_tag="ACHKTW_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239594.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(23152..23892)
                     /gene="yiaT"
                     /locus_tag="ACHKTW_002477"
     CDS             complement(23152..23892)
                     /gene="yiaT"
                     /locus_tag="ACHKTW_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0030674 - protein-macromolecule adaptor
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MipA/OmpV family protein"
                     /protein_id="HFO6463482.1"
                     /translation="MLINRNIVALFALPFMASATASELSIGAGAAYNESPYRGYNENT
                     KAIPLISYEGDSFYVRQTTLGFILSQSEKNELSLTASWMPLEFDPADNDDHAMQQLDK
                     RDSTAMAGVAWYHHERWGTVKASAAADVLDNSNGWVGELSVFHKMQIGRLSLTPALGV
                     LYYDENFSDYYYGISESESRRSGLASYSAQDAWVPYVSLTAKYPIGEHVVLMASAGYS
                     ELPEEITDSPMIDRNESFTFVTGVSWRF"
     gene            24016..24990
                     /gene="yiaU"
                     /locus_tag="ACHKTW_002478"
     CDS             24016..24990
                     /gene="yiaU"
                     /locus_tag="ACHKTW_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO6463483.1"
                     /translation="MTKLQLKYRELKIISVIAASENISHAATVLGIAQANVSKYLADF
                     ESKVGLKVFDRTTRQLMLTPFGTALLPYINDMLDRNEQLNNFIADYKHEKRGRVTIYA
                     PTGIITYLSKHVIDKIKDIGDITLSLKTCNLERNAFYEGVEFPDDCDVLISYAPPKDE
                     SLVASFITQYAVTAYASQRYLEKHPISRPDELEHHSCILIDSMMIDDANIWRFNVAGS
                     KEVRDYRVKGNYVCDNTQSALELARNHLGIVFAPDKSVQSDLQDGTLVPCFQHPYEWW
                     LDLVAIFRKREYQPWRVQYVLDEMLREIRHQLAQSQQLRPEQAAESED"
     gene            complement(24987..26123)
                     /gene="yiaV"
                     /locus_tag="ACHKTW_002479"
     CDS             complement(24987..26123)
                     /gene="yiaV"
                     /locus_tag="ACHKTW_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFO6463484.1"
                     /translation="MDLLIILTYVAFAWAMFKIFKIPVNKWTIPTAALGGIFIVSGLI
                     LLMNYNHPYTFKAQKAVISIPVVPQVTGVVIEVTDKKNTLIKKGEVLFRLDPTRYQAR
                     VDRLMADIVTAEHKQRALGAELDEMAANTQQAKATRDKFAKEYQRYARGSQAKVNPFS
                     ERDIDVARQNYLAQEASVKSSAAEQKQIQSQLDSLVLGEHSQIASLKAQLAEAKYNLE
                     QTIVRAPSDGYVTQVLIRPGTYAASLPLRPVMVFIPDQKRQIVAQFRQNSLLRLAPGD
                     DAEVVFNALPGKVFSGKLAAISPAVPGGAYQSTGTLQTLNTAPGSDGVIATIELDEHT
                     DLSALPDGIYSQVAVYSDHFSHVSVMRKVLLRMTSWVHYLYLDH"
     gene            complement(26129..26452)
                     /gene="yiaW"
                     /locus_tag="ACHKTW_002480"
     CDS             complement(26129..26452)
                     /gene="yiaW"
                     /locus_tag="ACHKTW_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HFO6463485.1"
                     /translation="MFLDYFALGVLIFVFLVIFYGIIILHDIPYLIAKKRNHPHADAI
                     HVAGWVSLFTLHVIWPFLWIWATLYRPERGWGMQNHDSSVVQLQQRIAGLEKQLADIK
                     SSSAE"
     gene            26635..26778
                     /locus_tag="ACHKTW_002481"
     CDS             26635..26778
                     /locus_tag="ACHKTW_002481"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6463486.1"
                     /translation="MNNSSKTSTVQIKRIKPSIIYRLLLIGLGAPMVIYGLIRPLTIE
                     TRD"
     gene            complement(26997..28535)
                     /gene="aldB"
                     /locus_tag="ACHKTW_002482"
     CDS             complement(26997..28535)
                     /gene="aldB"
                     /locus_tag="ACHKTW_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase AldB"
                     /protein_id="HFO6463487.1"
                     /translation="MTNNPPSAQIKPGEYGFPLKLKARYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDRAAILFKIADRMEQNLE
                     LLATAETWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMEIVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMERAIRRVESI
                     RSGNPLDSVTQMGAQVSHGQLETILNYIDIGKKEGADVLTGGRRKLLEGELKDGYYLE
                     PTILFGQNNMRVFQEEIFGPVLAVTTFKTMEEALELANDTQYGLGAGVWSRNGNLAYK
                     MGRGIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLEHYQQTKCLLVSYSDKP
                     LGLF"
     gene            complement(28700..29851)
                     /gene="yiaY"
                     /locus_tag="ACHKTW_002483"
     CDS             complement(28700..29851)
                     /gene="yiaY"
                     /locus_tag="ACHKTW_002483"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HFO6463488.1"
                     /translation="MASSTFFIPSVNVIGADSLTDAMNMMADYGFTRTLIVTDSMLTK
                     LGMAGDVQKALEERNIFSVIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHIK
                     MAIVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKV
                     DIPAGLRDLNVKEEDFAVLATNALKDACGFTNPIQATHEEIVAIYRAAM"
BASE COUNT         7457 a         7723 c         7492 g         7347 t
ORIGIN      
        1 ccccacccgg tcaggcaggg gataacgttt acgccattaa tggcagaagt tgctgataga
       61 ggcgacggaa cgtttcacgt cgtggctgat aagcggcata acgctgcgca tctggtagat
      121 gcgactgttc taacggtagc tgcggcaaca actcaatgag cgatttctct ggattcgccg
      181 caatctgcgc cagccttgct gcgcccagtg ctggccccac atcccccccc gtacggtaat
      241 cgagctgctg accgctaata tccgccagca tctgacgcca gtactcacta cgcgccccgc
      301 caccaatcaa cgtaacactt tgcggtttaa tgccgcaggc atgcacgaca tccatgccat
      361 ctgccagcgc ataacccacg ccttccagca ctgctcgcgc cagttcattg gggccatgtt
      421 gatgagtcaa accaaagaaa acccccttcg cctggggatt attgtgtggt gtacgctcgc
      481 cggaaagata aggcagaaac caaactggct cggcactttc atcagcctgt tgagctgcag
      541 cgattaaagc tgggacattg cacaggccgg ttaatttcgc gacccaatcc agacacgatg
      601 ctgcactcag cattacagac attaaatgcc aacgctgcgg tagcgcatgg caaaagctgt
      661 gtacagcgct ttctggcttg cttaagaacc cttcgctgac agcaaaatag acccccgacg
      721 tccccagcga taacattgcc tgattagcat caaccattcc cacaccaact gcaccagctg
      781 cattgtcgcc accgcctgcg acaactggca ccgtcgccat accccacgct ttcgcaactt
      841 caggtaacaa agcaccagta atttcgctgc cttcatataa tgcgggcatc tggtcacgag
      901 ataagtcgca agcctgcagc atgacgtcac tccagtcacg ctttgcgaca tccagccaca
      961 tggtgccagc tgcgtcagac atatcgctgg caaactcccc cgtcatacgc agacgcaagt
     1021 aatctttcgg taataatact ttgtcgattt gacggaatat ctccggctca tgccgctgaa
     1081 cccatagcaa tttaggcgca gtaaatccgg gcatcatcag gttgccggta atcactcgtg
     1141 attgcggaac tctcgcttcc agcaaagtgc actcttgcgc acagcgcccg tcgttccaca
     1201 aaatggcagg gcgcaatacc cgttgttgag catccagtaa ggttgctcca tgcatctggc
     1261 cggcaatacc caatgcttta acgtcctgca gagaatgctg atcgcccaga gctttcattg
     1321 cgcgatcagt tgcctgccac cactgttccg ggtcttgttc cgaccagagt ggatgcgggc
     1381 gcgaaacggt cagcttttcc gtttgcgaag caaccacctc accctgctcg ttgagcaaaa
     1441 taacttttac gcccgaggtg ccaagatcta tcccgatata catatcgatc gttccttaaa
     1501 aaaatgcccg gtatcgctac cgataaccgg gccaacggac tgcacagtta gccgttattt
     1561 gtcgaacaga taatgattta ccagattttc cagttgctcc tggcgaccac tctgatgcac
     1621 cggagacaaa ttatgttcct gagcatattt ggctaaatct gccagtgaca tttggccttt
     1681 caggatttgc tggcccaatt cgctattcca gccggaataa cgctgcgcga tgcgtttatc
     1741 cagctcgcca tcttcaatca tgcgcgctgc aattttcagc gccagtgcca tcgtatccat
     1801 cgcgccgata tgaccgtaaa acagatcata tttatcagta ctttgacgac gtactttggc
     1861 atcgaagttc agaccaccgg tggtgaaacc gcctgctttg agaatttcat acatcaccag
     1921 cgcattctct tccacactgt tcgggaactg gtcggtgtcc cagcccagtt gcgcatcgcc
     1981 acggttggcg tcgacagaac cgaacaggcc aagcgcaatg gcggtggcaa tttcatgatg
     2041 gaaagagtga cctgccagcg tcgcgtggtt agcttcaatg ttcagtttaa tctctttttc
     2101 cagaccaaac tgtttcagga agccatagac cgtcgcggca tcgtaatcat attgatgttt
     2161 ggtcggttct tgcggtttcg gttcgataag caacgtgccc tggaaaccga ttttatgttt
     2221 atgctcaacc accatctgca taaagcggcc cagctgttca cgctcctgac gcaagtcggt
     2281 atttaacagc gtttcgtaac cttcacgacc gccccacagg acatagtttt caccgcccaa
     2341 tttatgggtt gcttccatcg ctgtaacaac ttgcgttgcc gcccaactga agacttcagg
     2401 atctgggttc gtcgccgcac ccgcgccata gcgagggttt gtaaagcagt tagcggttcc
     2461 ccacagcagc ttcacgccgc tctcttcttg cttgcctgcc aggacatcaa ccatttgcgc
     2521 aaaattattg atgtactctt ttaacgacgc gccctcaggg gaaacatcca catcgtggaa
     2581 gcaataaaat ggcacatgta acttgtggaa aaactcaaat gcgacatctg ctttacgctt
     2641 cgccaacgcc agtgcctcac caggctgctg ccacggacga ttaaacgccc ccacaccaaa
     2701 catgtccgcc ccgttccagc agaatgtgtg ccagtagcag gcggcaaaac gcaagtgctc
     2761 ttccatacgc ttgcccaaca ccagttcgtc gggattgtag tgacggaatg ctaacgggtt
     2821 tgaggatttt gagccttcat aacgaacgcg atcgagctgg tcaaaatagg cttgcatatt
     2881 gaactccata atcaggtaat gccgcgggtg atggatgatg tcgtaatatt gggcactccc
     2941 tttcagttgc tcaattatgt tatttcacac tgctattgag ataattcaca agtgtgcgct
     3001 cgctcgcaaa ataaaatgga atgatgaaac tgggtaattc ctcgaagaga aaaatgcaat
     3061 aagtacaatt gcgcaacaaa agtaagatct cggtcataaa tcaagaaata aaccaaaaat
     3121 cgtaatcgaa agataaaaat ctgtaattgt tttcccctgt ttagttgcta aaaattggtt
     3181 acgtttatcg cggtgattgt tacttattaa agctgtcctc taactacaga aggccctaca
     3241 ccatgaaaat aaagaacatt ctactcaccc tttgcacctc acttctgctt accaacgttg
     3301 cggcacacgc caaagaagtc aaaataggta tggcgattga tgatctccgt cttgaacgct
     3361 ggcaaaaaga tcgagatatt tttgtgaaaa aggcagaatc tctcggcgcg aaagtatttg
     3421 tacagtctgc aaatggcaat gaagaaacac aaatgtcgca gattgaaaac atgataaacc
     3481 ggggtgtcga tgttcttgtc attattccgt ataacggtca ggtattaagt aacgttgtaa
     3541 aagaagccaa acaagaaggt attaaagtat tagcttacga tcgtatgatt aacgatgcgg
     3601 atatcgattt ctatatttct ttcgataacg aaaaagtcgg tgaactgcag gcaaaagccc
     3661 tggtcgatat tgttccgcaa ggtaattact tcctgatggg cggctcgccg gtagataaca
     3721 acgccaagct gttccgcgcc ggacaaatga aagtgttaaa accttacgtt gattccggaa
     3781 aaattaaagt cgttggtgac caatgggttg atggctggtt accggaaaac gcattgaaaa
     3841 ttatggaaaa cgcgctaacc gccaataata acaaaattga tgctgtagtt gcctcaaacg
     3901 atgccaccgc aggtggggca attcaggcat taagcgcgca aggtttatca gggaaagtag
     3961 caatttccgg ccaggatgcg gatctcgcag gtattaaacg tattgctgcc ggtacgcaaa
     4021 ctatgacggt gtataaacct attacgttgt tggcaaatac tgccgcagaa attgccgttg
     4081 agttgggcaa tggtcaggaa ccaaaagcag ataccacact gaataatggc ctgaaagatg
     4141 tgccctcccg cctcctgaca ccgatcgatg tgaataaaaa caacatcaaa gacacggtag
     4201 ttaaagacgg attccacaaa gagagcgagc tgtaagcgtt acgccccggc gcggagcggg
     4261 ggcgtgattt ctctccatgc cgcgtgaatg aattggctta ggtggagtcg ttatgcctta
     4321 tctacttgaa atgaagaata ttaccaaaac cttcggcagt gtgaaggcga ttgataacgt
     4381 ctgcttgcgg ttgaatgctg gcgaaatcgt ctcactttgt ggggaaaatg ggtctggtaa
     4441 atcaacactg atgaaagtgc tgtgtggtat ttatccccat ggctcctacg aaggcgaaat
     4501 tatttttgcg ggagaagaga ttcaggcgag tcacatccgc gataccgaac gcaaaggtat
     4561 cgccatcatt caccaggaat tggccctggt gaaagaattg accgtgctgg aaaatatctt
     4621 cctgggtaac gaaataaccc acaatggcat tatggattat gacctgatga cgctacgctg
     4681 tcagaagctg ctcgcacagg tcagtttatc catttcacct gatacccgcg ttggcgattt
     4741 agggcttggg caacaacaac tggttgaaat tgccaaggca cttaataaac aggtgcgctt
     4801 gttaattctc gatgaaccga cagcctcatt aactgagcag gaaacgtcgg ttttactgga
     4861 tattattcgc gatctacaac agcacggtat cgcctgtatt tatatttcgc acaaactcaa
     4921 cgaagtcaaa gcgatttccg atacgatttg cgttattcgc gacggacagc acattggtac
     4981 gcgtgatgct gccggaatga gtgaagacga tattatcacc atgatggtcg ggcgagagtt
     5041 aaccgcgctt taccctaatg aaccacatac caccggagat gaaatattac gtattgaaca
     5101 tctgacggca tggcatccgg tcaatcgtca tattaaacga gttaatgatg tctcgttttc
     5161 cctgaaacgt ggcgaaatac tgggtattgc cggactcgtt ggtgccggac gtaccgagac
     5221 cattcagtgc ctgtttggcg tgtggcccgg acaatgggaa ggaaaaattt atattgatgg
     5281 caaacaggta gatattcgta actgtcagca agccatcgcc caggggattg cgatggtacc
     5341 cgaagacaga aagcgcgacg gcatcgttcc ggtaatggcg gttggtaaaa atattaccct
     5401 cgccgcactc aataaattta ccggtggcat tagccagctt gatgacgcgg cagagcaaaa
     5461 atgtattctg gaatcaattc agcaactcaa agttaaaacg tcgtcccccg accttgctat
     5521 tggacgtttg agcggcggca atcagcaaaa agcgatcctc gctcgctgtc tgttacttaa
     5581 cccgcgcatc ctcattcttg atgaacctac caggggtatc gatattggcg cgaaatacga
     5641 gatctacaaa ttaattaacc aactcgtcca gcagggtatt gccgttattg tcatctcttc
     5701 cgaattacct gaagtgctcg gccttagcga tcgggtactg gtgatgcatg aagggaaact
     5761 aaaagccaac ctgataaatc ataacctgac tcaggagcag gtgatggaag ccgcattgag
     5821 gagcgaacat catgtcgaaa agcaatccgt ctgaagtgaa attggccgta ccgacatccg
     5881 gtggcttttc cgggctgaaa tcactgaatt tgcaggtctt cgtgatgatt gcagctatca
     5941 tcgcaatcat gctgttcttt acctggacca ccgatggtgc ctacttaagc gcccgtaacg
     6001 tctccaacct gttacgccag accgcgatta ccggcatcct cgcggtagga atggtgttcg
     6061 tcataatttc tgctgaaatc gacctttccg tcggctcaat gatggggcta ttaggtggcg
     6121 tcgcggcgat ttgtgacgtc tggttaggct ggcctttgcc acttaccatc attgtgacgt
     6181 tggttctggg actgcttctc ggtgcctgga acggatggtg ggtcgcgtac cgcaaagtcc
     6241 cttcatttat tgtcaccctc gcgggcatgt tggcatttcg cggcatactc attggcatca
     6301 ccaacggcac gactgtttcc cccaccagcg ccgcgatgtc acaaattggg caaagctatc
     6361 tacctgccag cactggcttc atcattggcg cgcttggctt aatggctttt gttggttggc
     6421 aatggcgcgg aagaatgcgc cgtcaggctt tgggtttgca gtctccggcc tctaccgcag
     6481 tagtcggtcg ccaggcttta accgctatca tcgtattagg cgcaatctgg ctgttgaatg
     6541 attaccgtgg cgttcccact cctgttctgc tgctgacgtt gctgttactc ggcggaatgt
     6601 ttatggcaac gcggacggca tttggacgac gcatttatgc catcggcggc aatctggaag
     6661 cagcacgtct ctccgggatt aacgttgaac gcaccaaact tgccgtgttc gcgattaacg
     6721 gattaatggt agccatcgcc ggattaatcc ttagttctcg acttggcgct ggttcacctt
     6781 ctgcgggaaa tatcgccgaa ctggacgcaa ttgcagcatg cgtgattggc ggcaccagcc
     6841 tggctggcgg tgtgggaagc gttgccggag cagtaatggg ggcatttatc atggcttcac
     6901 tggataacgg catgagtatg atggatgtac cgaccttctg gcagtatatc gttaaaggtg
     6961 cgattctgtt gctggcagta tggatggact ccgcaaccaa acgccgttct tgattttgat
     7021 aaaaattttc tcaaagccgg ttacgtatta ccggttttga gtttttgcat gattcagcag
     7081 gaaaagaacc atgtttacta aacgtcaccg catcacatta ctgttcaatg ccaataaagc
     7141 ctatgaccgg caggtagtag aaggcgtagg ggaatattta caggcatcac aatcagaatg
     7201 ggatattttc attgaagaag atttccgcgc ccgcattgat aaaatcaagg actggttagg
     7261 agatggcgtc attgccgact tcgacgacaa acagatcgag caagcgctgg ctgatgtcga
     7321 cgtccccatt gttggggttg gcggctcgta tcaccttgca gaaagttacc cacccgttca
     7381 ttacattgcc accgataact atgcgctggt tgaaagcgca tttttgcatt taaaagagaa
     7441 aggcgttaac cgctttgctt tttatggtct tccggaatca agcggcaaac gttgggccac
     7501 tgaacgcgaa tatgcatttc gtcagcttgt cgccgaagaa aagtatcgcg gagtggttta
     7561 tcaggggtta gaaaccgcgc cagagaactg gcaacatgcg caaaatcggc tggcagactg
     7621 gctacaaacg ctgccaccgc aaaccgggat tattgccgtt actgacgccc gggcgcggca
     7681 tattctgcaa gtatgtgaac atctacatat tcccgtaccg gaaaaattat gcgtgattgg
     7741 catcgataac gaagaactga cccgctatct gtcgcgtgtc gccctttctt cggtcgctca
     7801 gggcgcgcgg caaatgggct atcaggcggc aaaactgttg catcgattat tagataaaga
     7861 agaaatgccg ctacagcgaa ttttggtccc accagttcgc gtcattgaac ggcgctcaac
     7921 agattatcgc tcgctgaccg atcccgccgt tattcaggcc atgcattaca ttcgtaatca
     7981 cgcctgtaaa gggattaaag tggatcaggt actcgatgcg gtcgggatct cgcgctccaa
     8041 tcttgagaag cgttttaaag aagaggtggg tgaaaccatc catgccatga ttcatgccga
     8101 gaagctggag aaagcgcgca gtctgctgat ttcaaccacc ttgtcgatca atgagatatc
     8161 gcaaatgtgc ggttatccat cgctgcaata tttctactct gtttttaaaa aagcatatga
     8221 cacgacgcca aaagagtatc gcgatgtaaa tagcgaggtc atgttgtagc gatgatgaga
     8281 attgtcggcg tcacatcagg gaactcagca cagccacctg atgcgagcaa aagccttatc
     8341 cgaccgacaa atcagataag gctcaaggaa atgcaattac atatgcgccg cgattaaccg
     8401 ttggttatct tggtacattg cgaacaggta gttgttataa ctcttccctt tggtcgagta
     8461 gcccttcagc ttgtgaatca tcgctgtggc agtcacttcc tgatccgctt tacgcagctg
     8521 cgcacgcgat ttacggaacg aagagtaagc cgggtgcgta ttcaggttag tgacataggc
     8581 gctcaccgat tctttgacag aactaaactg tgagtaccct ttcactttac ccggcgcatt
     8641 ggtacaacgt cctttcatgc atttcatgcc gaacagattg ttattgttgc gcgccagctt
     8701 cgacgttccc caaccgcttt ctgctgcagc catcgtcgcc accatactgg tagggataat
     8761 gtctacgcgt tcaagcaagg tattccacgg gatttttcgc gtattaccag accacttcac
     8821 cttgtagcgt ttggcgatgt ctttcagacg cgcacgctca gcaggtgacc attggccctg
     8881 atactgtttt gaaatcagcc agttacgttc tgcagtaatg gctgcatttt ggctggtaat
     8941 gtaaggcatt acggtccgga gaaacgcctt tttccttggt gttccggaag ggtattttcg
     9001 caaatcagga agtgaactac tctttgcact attgcgagaa tactcttgtt tactgcttac
     9061 ctgtttatta ctagctttag ttaagtggga cttttgactc gctgttgttg tgtgcgtctt
     9121 cgctaacacc tcactcgaaa acaccagagt gagtaacata agaatcatcg ccccatatcg
     9181 tcgtatggga gtcaaaatca tcaggtctcc tggtcggatt taatcattcc aacaccttat
     9241 atttttcaca aatttgagag ttgaatctca aatcatatca aaaatagctg tcaagagcac
     9301 cccaaggaat agtccaaatc tgaaactatg tcacgtgtta acgattcaga ttggcgctaa
     9361 atcgcagaaa atgtggggtt tatcgcaaaa ttcagccgtt ttttgcgcga gatcgctcac
     9421 ccttgcttct catcctgtgg acttaccgct cagggatgag ttttgtctgg cttatcgctg
     9481 gcaaactgtc tgaaatcgca gcaataagga ctcatccacc atgaaactcg ccgcctgttt
     9541 tctgacactc cttcctggct tcgccgttgc cgccagctgg acttctccgg ggttccctgc
     9601 ctttagcgaa cagggaacgg gaacatttgt cagccacgcg cagttgccca aaggtacgcg
     9661 tccactcacg ctaaattttg accaacagtg ctggcagcct gcagatgcga taaaactcaa
     9721 tcagatgctt tccctgcaac cttgtagcaa cacgccgcct caatggcgat tgttcagaga
     9781 cggcaaatat acgctgcaaa tagacacccg ctccggtacg ccaacattga tgatttccat
     9841 ccagaacgcc gccgaaccgg tagcaaacct ggtccgtgaa tgcccgaaat gggatggatt
     9901 accgctcacg ctggatgtca gcgccacttt cccggaagga gccgccgtac gggattatta
     9961 cagccagcaa attgcgatag tgaagaacgg tcaaataacg ttacaacccg ctgctaccag
    10021 caacggttta ctcctgctgg aacgggcaga aactgacgcc cctgcccctt tcgactggca
    10081 taacgccacg gtttactttg tgctgacaga tcgtttcgaa aacggcgatc ccagtaatga
    10141 ccagagttac ggacgtcata aagacggtat ggcggaaatt ggcacttttc acggcggcga
    10201 tttacgcggc ctgaccaaca aactggatta cctccagcag ttgggcgtta atgctttatg
    10261 gataagcgcc ccatttgagc aaattcacgg ctgggtcggc ggcggtacaa aaggcgattt
    10321 cccgcattat gcctaccacg gttattacac acaggactgg acgaatcttg atgccaatat
    10381 gggcaacgaa gccgatctac ggacgctggt tgatagcgca catcagcgcg gtattcgtat
    10441 tctctttgat gtcgtgatga accacaccgg ctatgccacg ctggcggata tgcaggagta
    10501 tcagtttggc gcgttatatc tttctggtga cgaggtgaaa aaaacgctgg gtgaacgctg
    10561 gagcgactgg aaacctgccg ccgggcaaac ctggcatagc tttaacgatt acattaattt
    10621 cagcgacaaa acaggctggg ataaatggtg gggaaaaaac tggatcagaa cggatatcgg
    10681 cgattacgac aatcctggat tcgacgatct cactatgtcg ctggcctttt tgccggatat
    10741 caaaaccgaa tcaactaccg cttctggtct gccggtgttc tataaaaaca aaacggatac
    10801 tcacgctaaa gtcatcgaag gctttacacc tcgcgattac ttaacccact ggttaagtca
    10861 gtgggtccgc gactatggga ttgatggttt tcgggtcgat accgccaaac atgttgagtt
    10921 gcccgcctgg cagcaactga aaaccgaagc cagcgccgcg cttcgcgaat ggaaaaaagc
    10981 taaccccgac aaagcattag atgacaaacc tttctggatg accggtgaag cctggggcca
    11041 cggcgtgatg caaagtgact actatcgcca cggcttcgat gcgatgatca atttcgatta
    11101 tcaggagcag gcggcgaaag cagtcgattg tctggcgcag atggatacga cctggcagca
    11161 aatggcggag aaattgcagg gtttcaacgt gttgagctac ctctcgtcgc atgatacccg
    11221 tctgttccgt gaagggggcg acaaagcagc agagttatta ctattagcgc caggcgcggt
    11281 acaaatcttt tatggcgatg aatcctcgcg tccgttcggt cctacaggtt ctgatccgct
    11341 gcaaggtaca cgttcggata tgaactggca ggatgttagc ggtaaatctg ccgccaacgt
    11401 cgcgcactgg cagaaaatca gccagttccg cgcccgccat cccgcaattg gcgcgggcaa
    11461 acaaacgaca ctttcgctga agcagggcta cggctttgtt cgtgagcatg gcgacgataa
    11521 agtgctggtc atctgggctg ggcaacagtg acttttccgg cttcccgttc gtcagtacct
    11581 cgggaagccg ccaaccagga taaaatgtca gccctaatca gcgttgcagg ataaagcacc
    11641 gctcactctc caacagaccg atttgcaccc cagcaaatgt agcgttattg ttaccttcct
    11701 tgctacagag ttcgacagat atcccgctat gacattctcc ctttttggtg acaaatttac
    11761 ccgccactcc ggcattacgc tgctgatgga agatctgaac gacggtttac gcacgcctgg
    11821 cgcgattatg ctcggcggcg gtaatccggc gcagatcccg gaaatgcagg actacttcca
    11881 gacgctactg accgacatgc tggaaagtgg caaagcgact gatgcactgt gcaattacga
    11941 cggtccacag gggaaaacgg agctactcac actgcttgcc ggaatgctgc gcgagaagtt
    12001 gggttgggat atcgaagcac agaatattgc actaacaaac ggcagccaga gcgcgttttt
    12061 ctacttattt aatctgtttg ccggacgccg tgccgatggt cgggtcaaaa aagtgctgtt
    12121 cccgctggca ccggaataca ttggctacgc tgacgccgga ctggaagaag atctgtttgt
    12181 ctctgcgcgt ccgaatattg aactgctgcc ggaaggccag tttaaatacc acgtcgattt
    12241 tgagcatctg catattggcg aagaaaccgg gatgatttgc gtctcccggc cgacgaatcc
    12301 aacaggcaat gtgattactg acgaagagtt gctgaagctt gacgcgctgg cgaatcaaca
    12361 cggcattccg ctggtgattg ataacgctta tggcgtcccg ttcccgggta tcatcttcag
    12421 tgaagcgcgc ccgctatgga atccgaatat cgtgctgtgc atgagtcttt ccaagctggg
    12481 gctgcctggc tcccgctgcg gcattatcat cgccaatgaa aaaatcatca ctgccatcac
    12541 caatatgaac ggcattatca gcctggcacc tggcggtatt ggtccggcga tgatgtgtga
    12601 aatgattaag cgtaacgatc tgctgcgcct gtctgaaaca gtcatcaaac cgttttacta
    12661 ccagcgtgtt caggaaacta tcgccatcat tcgccgctat ttaccggaag atcgctgcct
    12721 aattcataaa ccagaaggag ccattttcct ctggctatgg tttaaggatt tgcccattac
    12781 gaccgagcaa ctctaccagc gcctgaaagc acgcggcgtg ctgatggtgc cggggcacaa
    12841 cttcttcccg ggactggata aaccgtggcc gcatacgcat caatgtatgc gcatgaacta
    12901 cgtaccagag ccggagaaaa ttgaggcggg ggtgaagatt ctggcggaag agatagaaag
    12961 agcctgggct gaaagtcact aaaaattgcc tgatgcgcta cgcttatcag gcctacatga
    13021 tttctgcaat atattgaatt tggaagaatt tgtaggccgg ataaggcgtt tacgccgcat
    13081 ctggcataaa caaagcgcac tattccggcg ttgagaaacg ccggaaaacg tttcaaacag
    13141 gctgctgccg tagccgatgc cgctgcactt tctcgacatc catgcactgc aacgcctttg
    13201 tcgggcaggc ttcaacacag gccggccccg tctcccgatg ccagcacaga tcgcacttaa
    13261 tcgccctcgc ctttttgcgc gaagaaacga cctccatcgc accaaacggg caagccagca
    13321 tacagctttt acagccaatg caacgtgttt gttcaacgaa aatatgccca tgttcgcggc
    13381 ttatcgcgtc aacagggcag acattcgcgc acggtgcatc ttcacactga tgacaggcta
    13441 ctgccgtggt ccagctgtgg tctttaatga cacgaatacg ggaaataaac tcgtctggtg
    13501 acaacgcagc gcaatcctga ttatcctgat gcgacactgc gcaagccact tcacaggtac
    13561 ggcaaccgat acatttcgtc gcatccgcaa taataaaccg gttcatgatc actcctttgc
    13621 actaaaacag cagagtgcca gagagcgcgt ccctactgct ttgatccaac gaaggaaatg
    13681 ttaaagtttt ggcacttgtc caaagcgtta tgtgatcgcg ccctggtcgt ccctgacagt
    13741 aaatcccagt tcattagaaa tagcctgcgc ggtttcacgc agtggtttca ggagattttt
    13801 ctcccccacc tgtttcagac gtgatgtcga aagcgaaatc gacacggcgt acggcacccg
    13861 cccatgaata ttaaacaccg gaacagcaat acaggagacg ccgagttcgt tttcttctct
    13921 gtccatcgcc gctccgcttt cacgaatgtg cgccagttcg tcgaacatcg cgggcagctc
    13981 ggtaatggta ttgcgggtta acggctggat ctcatgctga tggttttccc agtatgactt
    14041 cacgtagtcc gggtgaccaa acgccatgta gatcttgccc attgcggaac agtagagcgg
    14101 catatgctgg ccaatatagg cacgggttcg cagcatcccg gttgtgggtt ccagcttata
    14161 aatcaaaata gcgtgatcgt cttcgcggct ggagaagtta atggtttcac cagtggcgat
    14221 gttcagtgcc tcaagatgcg gagcggcgat atgaatgata ttcagcgaag acagcgcctt
    14281 ctgcccgacg gcaataaatt tggtagtcag gcggtaactc cctgcggcgg gcgcggtggt
    14341 cacatagccg caggactgta atccctgcaa taagcgatgg acggtactct tatttaaacc
    14401 agccagctcc gaaagatgcg ccaacggaca accgtttgga tagttgctca aaatctcaat
    14461 cagcatcaac ccgcgaaaca gactctggct tccggctgga cgctcttttt cctgcgccat
    14521 ctcgttctct ttttttccca tcacttcttt ccccattttg tcgcgtcctg atggtagcgc
    14581 aaagtgtgcc gtagttcacg atctcgacag ataatttata accacttgat ttttatgtat
    14641 tttgaaattc atcaatcaga ttgctttgtt aaaaagtgat cgatatattt gaaatcaagt
    14701 ttcgcatatt gaaattttaa gccaaaaaag cgatcaaaaa aacaaggaag cctgggatga
    14761 aagtgacatt tgagcagtta aaagcagcct ttaatcgggt cttaatttca cgcggcgttg
    14821 acagcgaaac ggctgacgcc tgtgcagaga tgttcgcccg caccaccgaa tccggcgttt
    14881 attctcacgg cgttaatcgt ttccctcgtt tcattcaaca actggaaaac ggcgatatca
    14941 ttcctgatgc ccaacccaaa cgtataacca gcctcggcgc aattgaacag tgggatgccc
    15001 agcgttcgat cggcaacctg acggcgaaaa agatgatgga tcgcgccatt gaactggctg
    15061 ccgatcacgg tattggtctg gtggcacttc gtaatgccaa ccactggatg cgcggcggca
    15121 gctacggctg gcaggcggcg gaaaaaggct atattggcat ttgctggacc aactccatcg
    15181 ccgtaatgcc gccgtggggc gcaaaagagt gtcgcattgg cactaacccg ctgatcgtcg
    15241 ccattccttc cacgccgatc accatggtcg atatgtcgat gtcgatgttc tcttacggca
    15301 tgttagaagt taaccgcctg gcgggccgtc agctcccggt cgatggtggc tttgatgatg
    15361 agggcaattt gaccaaagaa cctggcgtta tcgagaagaa tcgccgcatt ttgccgatgg
    15421 gctactggaa aggttctggc atgtcgattg tgctggatat gatcgctact ctcctttccg
    15481 acggcgcatc ggttgccgaa gtcacccagg acaacagcga cgaatacggc atttcacaaa
    15541 tttttattgc cattgaagtg gacaagctta tcgacggtcc cacccgcgat gccaagctgc
    15601 aacgtatcat ggattacgtt actaccgctg aacgcgctga cgaaaaccag gccatccgct
    15661 tacccggcca cgaatttact accctgctgg ccgaaaaccg ccgtaacggt attaccgttg
    15721 atgacagcgt gtgggccaaa atccaggcgt tataaggaga taagtcatga tttttggaca
    15781 tatcgcgcag cccaatccgt gccgtttgcc cgccgccatt gaaaaggcgc tcgattttct
    15841 gcgcgctacc gatttcaacg ccctggagcc gggcgttgtc gaaatcgacg gcaaaaatat
    15901 ttatgcgcaa attatcgatt taaccacacg cgaagcggtg gaaaaccgtc cggaagtgca
    15961 tcgtcggtat atcgatattc agtttctggc gtggggtgaa gagaaaatcg gcattgctat
    16021 tgatacggga aataataaag tcagcgaatc actattagag cagcgcgata ttatttttta
    16081 tcatgacagt gaacatgaat catttataga aatgataccg ggcagctacg caattttctt
    16141 tccgcaggat gttcaccgac caggttgtat tttgcaaaca gcctctgaga tacgaaaaat
    16201 tgtggttaaa gtcgcattaa cggcgctgaa ttaatcgaat aatcgtctac gccagaacga
    16261 ctgggtaatg ttattgctct aactatatta ataatttagg ttgctgcaca tgcaacttga
    16321 attatgacag acatagcctc aaggaatagc tatgaaaaaa atacttgaag caatactggc
    16381 gattaatctc gccgtacttt cctgtattgt gtttataaac attattttaa gatatggttt
    16441 tcagacaagc attttatctg tagatgaatt gtcacgttat ttatttgtct ggttaacatt
    16501 tattggcgcg attgtggctt ttatggataa cgcccacgtt caggtcacct ttctggtgga
    16561 gaaactctcc cccgcatggc aacgacgagt cgctctggtt acccattcct taatcttatt
    16621 tatttgtggc gcactggcct ggggcgcaac gctaaaaaca attcaggact ggagcgatta
    16681 ttcaccgatc ctcggtttac ccatcggcct gatgtatgcc gcttgtctgc ccaccagcct
    16741 cgttatcgca ttctttgaac tgcgtcattt atatcaactc atcacgcgta gcaattctct
    16801 aacgtcgcca ccgcaaggag cttaatcatg gctgtgctga tttttctggg ctgtctgttg
    16861 ggtggtattg ctatcggttt gcctattgcc tgggcactgt tgttgtgcgg ggcggcgtta
    16921 atgttctggc tggacatgtt tgatgtccag atcatggcac aaacgctggt gaacggagcc
    16981 gatagcttct ccctgctggc gattccgttc tttgtgctgg cgggtgaaat catgaatgcg
    17041 ggcggcttgt caaaacgcat tgttgacctg ccgatgaaac tggtggggca taaaccaggc
    17101 gggctgggct acgtcggcgt gctggcagct atgattatgg cgagcctttc tggttctgcc
    17161 gttgcggata ccgccgccgt tgccgctcta ctggtgccga tgatgcgcag cgccaactac
    17221 ccggtcaacc gggcggcggg gctgattgcc tctggcggca ttatcgcgcc aattattccc
    17281 ccttccattc cgtttattat cttcggcgtt tccagcggat tatccatcag taagctgttt
    17341 atggcgggca ttgcccctgg catgatgatg ggcgcaacgc tgatgcttac ctggtggtgg
    17401 caggccagtc gccttaattt accccgccag caaaaagcaa cgatgcagga aatctggcac
    17461 tcttttgtct ccggtatctg ggcgctgttt cttccagtca tcattattgg cgggtttcgc
    17521 tccggtctgt ttacccctac cgaagccggg gccgttgctg ctttctatgc gctgtttgtc
    17581 gccacagtta tttaccgtga aatgaccttc gccacacttt ggcatgtact cattggcgcg
    17641 gcgaaaacca cctcagtggt tatgtttctg gtggcctcgg cacaagtttc cgcctggctg
    17701 attaccattg ctgaactgcc gatgatggtt tctgatttac tgcaaccgtt ggtcgattca
    17761 ccgcgtctgc tgtttatcgt cattatggtg gctatcctga ttgtcggcat ggtcatggat
    17821 ttaacgccga cggtattaat tcttaccccg gtattaatgc ctttagttaa agaggcagga
    17881 atcgatccga tttatttcgg tgtcatgttt atcattaact gctcaatcgg cttaattaca
    17941 ccgcctatcg gtaatgtact taacgttatt tcaggggtgg caaaactcaa attcgatgat
    18001 gcagtcagag gcgttttccc ttacgtcctg gttttatatt cattattagt cgtatttgtt
    18061 tttattcccg atctcatcat cctccctttg aaatggatta attaaaagga aaatattatg
    18121 aaattacgct ctgtaaccta cgcattattc attgctggcc tggctgcatt cagcacatct
    18181 tctctggcgg cgcaatcttt acgtttcggt tatgaaacct cacaaaccga ctcgcaacat
    18241 attgcggcga aaaaattcaa tgatttattg caggagagaa ccaaaggcga gctgaaatta
    18301 aaattgtttc cggatagcac cctcggtaac gcgcaggcga tgatcagcgg cgtacgtggc
    18361 ggcaccatcg atatggaaat gtccggctcg aataactttg ccgggttatc accagtgatg
    18421 aacttgcttg atgtcccttt cctgttccgc gataccgctc acgcgcataa aacgctcgac
    18481 ggcaaagtcg gtgatgatct gaaagcctca cttgaaggta aaggactaaa agtactggcc
    18541 tactgggaaa acggctggcg cgatgtcacc aactcgcgcg caccggttaa aacccccgcc
    18601 gacctgaaag ggctgaaaat tcgcaccaac aatagcccga tgaatatcgc cgcattcaaa
    18661 gtctttggcg ctaacccgat cccgatgcca tttgccgaag tctataccgg gctggaaacg
    18721 cgcactatcg acgctcagga acacccgatc aatgtcgtct ggtcagcaaa atttttcgaa
    18781 gtgcagaagt acctttctct gacgcaccac gcctattccc cgcttctggt ggtgatcaac
    18841 aaagcgaagt ttgatggctt aaccccggag ttccagcaag cgctaatttc atctgcgcag
    18901 gaagcgggta actaccagcg caaactggtt gccgaagatc agcaaaaaat catcgacggc
    18961 atgaaagaag cgggcgtgga agtcatcacc gatctcgacc gcaaagcctt tagcgacgca
    19021 ctgggtactc aggtccgcga catgtttgta aaagatgttc cgcagggtgc tgatctactg
    19081 aaagccgtgg atgaggtgca ataaaccatg acgcaatact ggctggggtt agattgtggc
    19141 ggtagctggc tgaaagccgg gctgtatgac cgcgaaggcc gggaggcagg cgtgcagcgc
    19201 ctgccgctgt gcgcattaag cccgcagcca ggttgggcag agcgcgatat ggcagaactg
    19261 tggcaatgct gcatggctgt cattcgcgaa ctgcttaccc attccggcgt tagcggggaa
    19321 caaattgtcg gtatcggtat ctccgcacaa ggaaaaggct tgtttttgct ggataaaaac
    19381 aacaagccgc tcggtaacgc cattttgtcc tcggatcgcc gggcgatgga aatcgttcgt
    19441 cgctggcagg aagatggcat cccggaaaaa ctctacccgt tgacccgaca aaccttgtgg
    19501 accgggcatc cggtgtcgct gttacgctgg ctgaaagagc acgaaccgga acgctacgcg
    19561 caaattggct gcgtgatgat gacgcacgac tacctgcgct ggtgtttaac cggcgtcaaa
    19621 ggctgtgaag agagcaatat ttccgagtcc aacctctaca acatgagtct tggggaatat
    19681 gacccgtgcc tcaccgactg gctggggatt gctgaaatca atcacgccct gccgcctgtt
    19741 gtcggatctg ccgaaatctg cggggagatc accgctcaga cagccgtact gaccggtctg
    19801 aaagcgggta cgcccgttgt cggcggcctg tttgatgtgg tttccaccgc actctgcgcc
    19861 gggatcgaag acgaatttac ccttaatgcg gtgatgggca cctgggcggt gaccagcggc
    19921 ataacccgcg gtttacgtga cggtgaagcg catccgtatg tctatggtcg ctacgttaac
    19981 gatggtgaat ttatcgttca cgaagccagc cccacctctt ccggcaacct cgaatggttt
    20041 accgcacagt ggggagaaat ctcgtttgat gagatcaatc aggccgttgc cagcttgccg
    20101 aaggctgggg gcgatctctt tttcctgccg ttcctgtacg gcagcaacgc cggactcgag
    20161 atgaccagtg gtttctacgg gatgcaggcc attcacaccc gcgcgcacct gttgcaggcc
    20221 atctatgaag gggtggtgtt cagccatatg acccacctca accgaatgcg cgaacgtttt
    20281 actgatgttc acaccctacg cgtcactggc ggcccggcgc actccgatgt ctggatgcaa
    20341 atgctggcgg acgtcagcgg tctgcgtatc gagctgccgc aggtggaaga aaccggctgc
    20401 tttggtgcgg cccttgccgc ccgcgtcggc accggggttt atcacaactt cagcgaagcc
    20461 caacgtgact tgcgacaccc ggtgcgcacc ctgctgccag atatgaccgc ccatcagctt
    20521 taccaaaaaa aatatcaacg ttatcagcat ctcattgccg cacttcaggg ctttcacgcc
    20581 cgcattaagg agcacacatt atgagccgac cacttctgca actggccctc gaccactcat
    20641 cacttgaagc cgcgcagcgc gacgtgacgc tgttaaaaga cagcgtcgat atcgtcgaag
    20701 cgggcatcat tctctgttta aacgaagggc ttggcgcggt gaaagccttg cgcgaacagt
    20761 gcccggacaa aatcatcgtt gctgactgga aggtcgccga cgctggtgaa acgctcgcgc
    20821 aacaggcgtt tggcgcaggc gctaactgga tgaccatcat ctgcgccgcg ccgctcgcga
    20881 cggtagaaaa aggccacgca atggcgcaac gctgcggggg tgaaattcag atagagctgt
    20941 tcggtaactg gacgctggac gacgcccgcg actggcatcg tattggcgtg cggcaggcca
    21001 tttatcatcg cggtcgtgat gcacaggcca gcgggcaaca gtggggcgaa gccgatctgg
    21061 cacgcatgaa ggcgctttca gatatcggcc ttgagctttc cattactggc gggattaccc
    21121 ctgctgacct gccgctgttt aaagatatcc gcgtgaaagc gtttattgcc gggcgggcac
    21181 tggcaggcgc ggcaaatccg gcgcaagtgg ctggcgattt ccatgcgcaa atcgacgcta
    21241 tctggggagg tgcgcgtgcg taatcatccg ttagggattt atgaaaaagc gctggcgaaa
    21301 gatctctcct ggccggagcg gctggtgctg gcaaaaagct gcggttttga ttttgtcgaa
    21361 atgtcggtgg atgaaaccga cgaacggctc tcacgtcttg actggagcgc cgcacaaagg
    21421 acttcgctgg ttgccgcgat gatcgaaaca ggtgttggga tcccgtcgat gtgcctgtcc
    21481 gcacatcgtc gctttccctt tggtagccgt gacgaggcag tgcgcgaacg ggcgcgggaa
    21541 atcatgagta aagcgattcg cctggcgcgc gatctcggca ttcgcaccat ccagctggca
    21601 ggttatgacg tctattacga agaccacgac gaaggcaccc ggcaacgttt tgctgaaggg
    21661 ctggcgtggg cagtcgaaca ggcggcggca tcacaagtaa tgctggcggt ggagattatg
    21721 gatacagcgt ttatgaactc catcagcaaa tggaaaaaat gggacgagat gctcgcctcg
    21781 ccgtggttca ccgtctaccc ggacgtcggc aacctcagcg cctggggcaa tgatgttccc
    21841 gccgaactga aactgggcat tgaccgtatc gccgcgatcc acctgaaaga cacccagcca
    21901 gtcaccgggc aaagccccgg acagttccgt gatgtgccgt ttggcgaagg ctgcgtcgat
    21961 ttcgttggca tcttcaaaac gctgcatgaa ctgaactatc gcggttcttt cctgattgag
    22021 atgtggaccg aaaaagccaa agagccggtg ctggagatca ttcaggcgcg gcgctggatt
    22081 gaagcgcgta tgcaggaggc tggatttata tgttagagca actgaaagcc gacgtgctgg
    22141 cggcgaatct ggcgcttccc gctcaccatc tggtgacgtt cacctggggc aatgtcagcg
    22201 cggtagacga cacgcggcaa tggatggtaa tcaaaccttc cggcgtcgag tacgacgtga
    22261 tgaccgccga cgatatggtg gtggttgaga tagccagcgg caaggtggtg gaaggtagca
    22321 aaaagccctc gtccgatacg ccaacgtatc tggcacttta ccgtcgctat gccgaaattg
    22381 gtggcattgt gcatacccac tcgcgccacg ccaccatctg gtcacaggcc gggctggatc
    22441 tccccgcctg gggcaccacg cacgctgatt acttctatgg tgccatcccc tgcacacgcc
    22501 tgatgaccgc agaggagatc aacggcgagt atgaatacca gaccggcgaa gtgatcattg
    22561 aaactttcga agaacgcggc aggagtccgg cacaaatccc ggcggtactg gtacattctc
    22621 acggcccgtt cgcctgggga aaaaatgccg ccgatgcggt gcataacgcc gtggtgctgg
    22681 aagagtgtgc ctatatgggc ctgttctcgc gccagcttgc gccgcagctc cctgcgatgc
    22741 aaaacgaact gctggataaa cactacctgc gtaagcatgg agacaatgcc tattacgggc
    22801 agtaatccct cacgccgggg cttcatcgcc ccggcactac gaattgatat gttccttgct
    22861 gtaacgccgc ttccacgctg ctggcgttaa accggtgtgc ttgcgaaaaa tctgccggaa
    22921 atagccaacc tcattaaacc gtattgcatg gagcaggccg acaacgtcga gtcactgcat
    22981 aacctgttgc tgcccgccga tgcgccattt acgacatttg aaggcaaggg attgttcagc
    23041 cataagatcc tgattcagac gccaggttcc cggtatgaac agagcaatcc agagcaacaa
    23101 cctttgtggc attacgacgg agcaccagcc gcaccgacat ccaccggtga attaaaaacg
    23161 ccagctcacg ccggtgacaa aggtaaaact ctcattacga tcaatcatcg ggctgtcggt
    23221 aatctcttcc ggcagctcgc tgtatccggc gctcgccatc aatactacgt gctctcctat
    23281 cgggtatttt gccgtcaggc tgacataggg cacccaggca tcctgcgcgg aataacttgc
    23341 cagaccgcta cgacgggact cactctctga aatgccatag taatagtcac tgaaattctc
    23401 gtcataatag agaacgccca gcgcaggtgt cagcgacagg cgacctatct gcattttgtg
    23461 gaaaaccgat agctccccca cccagccgtt gctgttatcc agaacgtccg cagctgcaga
    23521 ggctttcacg gttccccaac gctcgtggtg ataccaggca acccccgcca tagccgtact
    23581 atcacgctta tcaagttgtt gcatggcatg atcgtcatta tcggcagggt caaattccag
    23641 cggcatccag gatgcggtca ggctaagttc atttttttca ctttgcgaca gaataaaacc
    23701 taacgtggtc tgacgaacat aaaaagaatc accttcataa ctaatcaggg gaattgcctt
    23761 tgtattttca ttataaccgc gataaggcga ttcattataa gccgcaccgg caccaatgga
    23821 taattcagaa gcagttgcgc ttgccataaa aggcaacgca aataacgcca caatattgcg
    23881 gttaattaac atgtccggta ttccatttta aaataagata aaaaagagtc ggcatagtaa
    23941 tgcttacaat ttctccggtg caagattcat atttatatag taatatataa aattatatat
    24001 aattgggctg ttgcgatgac aaaattacaa cttaagtacc gggagttaaa aattatctcg
    24061 gtaatcgctg ccagtgaaaa tatcagccat gccgcgactg tacttggcat cgcacaggcc
    24121 aacgtcagca aatatcttgc tgattttgaa tcaaaagtgg gtttaaaagt ctttgaccga
    24181 accacccggc agctgatgct cacacctttt ggcaccgcgc tgttaccgta catcaacgac
    24241 atgctggata gaaacgagca acttaataat tttattgcag attataagca tgagaaacgt
    24301 ggtcgggtca ctatctacgc cccaaccggc ataattacct atttatccaa acatgtaatc
    24361 gataaaatta aagatatcgg tgatatcacc ttatcgctaa aaacctgcaa tcttgagcgt
    24421 aatgcctttt atgaaggcgt cgaatttcct gatgattgtg atgttttaat cagctacgct
    24481 ccaccaaaag atgaatcgct ggttgccagt tttattaccc aatatgccgt aaccgcttat
    24541 gccagccagc gctatcttga gaaacatcct attagccgtc ctgacgaact cgaacatcac
    24601 tcctgtattt tgatcgactc gatgatgatt gacgacgcga atatctggcg cttcaacgtc
    24661 gccgggagta aagaggtgcg ggattatcgc gtgaagggaa attacgtttg cgataacacg
    24721 caatcggcgc tggagctggc acgaaatcac ctggggattg tgtttgcgcc agacaaaagt
    24781 gtgcagagcg acctgcaaga cggcacgctg gtgccctgct ttcagcaccc gtatgaatgg
    24841 tggctggatc tggtggctat cttccgtaaa cgggaatacc agccctggcg ggtccagtat
    24901 gtgctggatg aaatgctgcg tgaaattcgc caccagcttg ctcagtcgca gcaactgcgg
    24961 cctgaacaag cggcggaaag cgaagattaa tgatcgagat aaaggtaatg cacccaactg
    25021 gtcatgcgta acagcacttt gcgcatcacc gagacatggc tgaaatgatc agagtagacc
    25081 gccacctggg agtaaatacc gtctggtaaa gcgctcaaat cagtgtgctc atccagttca
    25141 atggtcgcga taacgccatc tgaacccggc gctgtgttta acgtctgtaa ggtgccggtc
    25201 gactgataag ctccgccggg aacagctgga ctaatggctg ccagcttgcc gctgaatacc
    25261 ttacctggca gagcgttaaa taccacttcc gcatcatcgc caggggccag tcgcagcaag
    25321 gagttctgac ggaactgcgc cacaatttgt cgtttctgat cgggtataaa caccatcacc
    25381 ggacgtagcg gcagcgacgc ggcataggta cccggacgaa tcagcacctg ggtcacataa
    25441 ccatcgctcg gcgcacgcac tatcgtctgc tcaaggttat attttgcttc cgcgagctgc
    25501 gctttcaggc tggcgatttg agaatgttca cccaacacca ggctatccag ctggctctgg
    25561 atctgttttt gttccgccgc cgatgacttt acggaggctt cctgcgccag ataattttgc
    25621 cgcgccacat caatatcgcg ttctgaaaac gggtttactt tcgcctggct gccgcgtgcg
    25681 taacgctgat actctttagc gaatttatcc cgcgtggcct ttgcctgctg agtattcgcc
    25741 gccatctcat ctaactctgc gcccaacgcc cgctgtttat gttctgcggt aacgatatcc
    25801 gccatcagcc gatccacccg cgcctgataa cgcgtcgggt ccagtcgaaa tagcacctca
    25861 ccttttttaa tcagcgtatt tttcttatcc gtcacttcga tcaccacgcc tgtcacctgt
    25921 gggacaacag gaatagaaat aaccgctttt tgcgctttga aggtatacgg atggttatag
    25981 ttcattaaca gaattaaacc actgacaata aatatgccac ccagggccgc tgtgggaatg
    26041 gtccatttat ttacaggaat tttgaagatc ttaaacattg cccatgcgaa agccacatag
    26101 gtcaaaataa tcagtaaatc cataagtatt actcggcaga agaggatttg atgtcggcga
    26161 gctgtttctc cagcccggca atacgctgtt gtagctgcac aacggaggag tcatggtttt
    26221 gcattcccca tccccgctcc gggcggtaaa gcgtggccca aatccacaaa aatggccaga
    26281 taacatgtaa cgtaaaaaga ctcacccaac cagcaacatg aatggcgtcg gcatgaggat
    26341 gattacgttt tttagcaatc aggtagggaa tatcatgtaa aataataatc ccatagaaga
    26401 tcaccagaaa tacaaaaata agcactccca gtgcaaaata gtccaggaac atatttccct
    26461 cagagaatat aaaaaagaac aattaacgca tattatgaaa tgccatgatg cagtgcaatt
    26521 tccttattta tataacgaca tattgttttt tatatacttt ctgaagtgtg ctacctggct
    26581 tttcaacagg tcgagactta tcatattgaa ataatggaac tattggccaa actaatgaat
    26641 aactccagta aaacatccac agtacagatt aagcgtatta aaccttcaat tatctaccgt
    26701 ttattgctga ttggcctcgg agcaccaatg gtgatttacg gcctgattcg cccgctcacc
    26761 atcgaaacgc gagattaaac tcctgacaaa acacgcccca gaaacgcact tctgttggcg
    26821 tagtctgggt tattgcgcac gtaggtttaa aacgtaaatt gtaacaacgt gcgctttgtt
    26881 tatgccggat gcggcgtgta cgccttatcc ggcctacggt caggttcccg taggcatgat
    26941 aagacgcgta agcgtcgcat caggcaatga atacccaatg cgaccagctt cttatatcag
    27001 aacagcccca acggtttatc cgagtagctc accagcaggc acttggtttg ctggtaatgc
    27061 tccagcatca tcttgtgggt ttcgcgaccg atacctgatt gtttgtagcc accaaacgca
    27121 gcatgtgccg ggtaagcgtg atagcagttg gtccacacgc gcccggcctg aatgccgcgc
    27181 cccatcttat aggccagatt accgttacga ctccagacgc ctgcgcccag gccatattgc
    27241 gtatcgttcg ccagctccag cgcttcttcc atcgttttga aggtggtcac cgccagcacc
    27301 gggccaaaaa tctcctcctg gaacacccgc atattgttct gaccaaacag aatcgtcggt
    27361 tcgaggtagt agccgtcttt cagttcacct tccagcagct tgcgccgccc gccggtgagc
    27421 acgtctgcgc cctctttttt accgatatca atgtagttga ggatggtttc cagttgcccg
    27481 tgagaaacct gcgcgcccat ttgcgtcacg ctgtcgagtg ggttaccgct gcgaatgctt
    27541 tcgacacggc ggatggcgcg ttccataaag cgttcgtaga ttgactcctg cactaaggca
    27601 cgactcggac aggtgcaaac ttcaccctgg ttaaaggcaa acagtgcaaa gccttccagc
    27661 gctttatcga aaaaggcatc ttcttcatcc atcacatcag caaagaagat atttggcgat
    27721 ttgccgccca gctccagcgt caccggaata atgttttgcg tggcgtattg cataatttgt
    27781 tggcccactt ccgttgagcc ggtaaacgcc actttggcga tgcgtttcga ggtcgccaga
    27841 tattcgccaa tttccccacc tgcgccgttg accacgttca ccacgcccgg cggcagtaaa
    27901 tcaccgacga tttccattag cagcagtaca gaaagcgggg taagacgtgc gggtttcagc
    27961 accacacagt tgcccgccgc cagcgcggga gccattttcc agctcgccat cagcagcggg
    28021 aagttccacg ggataatctg ccccaccacg cctaacggtt catggaaatg ataggccacg
    28081 gtttcgctat caacttcact gatcccacct tcctgcgccc gaatacacga ggcgaaatag
    28141 cggaaatggt caatcgccag cggtacatcc gcagcactgg tttcgcgaat gggtttgccg
    28201 ttatcccagg tttcagctgt cgctaacagc tcgaggtttt gttccattcg atcggcaatc
    28261 ttaaacagaa tcgccgcgcg atcctgcacc gaggtgtgcg cccatttatc tttcactttg
    28321 tgcgcagcat ccagcgccag atcgatgtct cgtttgcccg aagacgccac ttcgcacagc
    28381 agctgcccgg tcaccggcgt cagattctgg taatactcgc cgtcggcagg ggctacccat
    28441 tcgccgccaa taaagttgtc atagcgggct tttaacttga gggggaaacc atactcgccg
    28501 ggcttaatct gtgctgaagg gggattattg gtcatgatcg tctccttgcg gtgtgaggta
    28561 taacaagggt agacgtgact ggcgaaatct tcgccagtcg gtaacagctt tacgacagct
    28621 atcacgaatt tacgggcaag ggttacagag atagttgaaa aagcggctga caatttgcca
    28681 gccgttgtgg aaatgatgat tacatcgctg cgcgataaat cgccacaatt tcttcgtgag
    28741 ttgcctggat cgggttagta aaaccacagg catctttcag ggcattagtc gccagaaccg
    28801 cgaaatcttc ttctttcacg ttcaggtcgc gtaggcctgc cgggatatcc actttcttcg
    28861 ccagttcacg gatggcgtta atgcaggctt cagcaccttc cgcatcgttt ttacctgtca
    28921 cgttcacgcc cattgcagcg gcacagtcac gcagacgtgc ggcggcgact ttgctgttga
    28981 atacctgaac atgcggcagc aaaacggcgt tacatacacc gtgtggcagg ttgtagaaac
    29041 cgcccagctg gtgcgccatc gcatgaacat aacccagaga agcattattg aacgccatac
    29101 cggcgaggaa ctgggcataa gccattgctt cacgcgcttt cgcattactg ccatcttcaa
    29161 cggctaacgg caggttttcg gcaatcatgg ttacggcttt cagtgcacaa gcgtcagtga
    29221 tcggcgtggc ggcaatagaa acatacgctt cgatagcgtg cgttaaggca tccatacccg
    29281 ttgcggcggt cagtgacttc ggcataccaa tcatcagaga ggagtcattg acagaaagca
    29341 gcggagtgac atgtttatca acaatcgcca ttttgatatg acgcgcttcg tcagtgatga
    29401 tgcagaaacg ggtcatttca gaggccgtac ccgccgtggt attgatggcg atcatcggca
    29461 gctgcggttt tgcagagcgg tcaacgcctt cgtaatcgcg aatatcgccg ccattggctg
    29521 ccaccagcgc aatacctttt gcgcagtcgt gtggagaacc accgcctaag gagatcacgc
    29581 tatcgcaatt attctcttta agtaatttca aacctgcggc gacgttttcc gtggtggggt
    29641 taggttgggt gccatcataa ataacgctaa aaatattgcg ttcttccagt gctttttgca
    29701 catcgcccgc catacctaat ttcgttaaca tactgtcagt gacaattaag gtacgggtaa
    29761 atccataatc tgccatcata ttcattgcat cagtcaatga atcagcgccg atgacattca
    29821 cagaaggaat aaagaaagtt gaagatgcca taacacactc ctgaaagtgt aaaaaagggt
    29881 gggcagctta ctgaggattt cattcgaaaa catgatcatt attattgatt atcaaaatta
    29941 atctaataaa aagcacattg tattcattaa acaatgtgct ttttttaaaa tcataagaaa
    30001 tttgtattaa tttatttta
//
LOCUS       DAZFQD010000056        28792 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.056, whole genome shotgun sequence.
ACCESSION   DAZFQD010000056 DAZFQD010000000
VERSION     DAZFQD010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28792
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.056"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(82..1053)
                     /locus_tag="ACHKTW_002484"
     CDS             complement(82..1053)
                     /locus_tag="ACHKTW_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HFO6463489.1"
                     /translation="MKIKVSAGIIGAVLMLSASQSWAVTLKLSHNQDKSHPVHKAMEF
                     FAKKSKEYSNGDITIRIYPNGTLGTQRETMELIRSGAIPLVKTNAAEMEAFENSYKLF
                     SLPYLFRDRDHYYQVMQGDIGRKILDSTKSKGYFGLTFYDGGARSFYGNKPVLKPDDL
                     KGMKVRVQPSPGAVEMIKVMGGNPTPLDYGELYTALQQGVVDMAENSVMALTTMRHGE
                     VAKSFSLDEHTMVPDVVLMSNAAFDKLSPENQEVILKAAKESMSYMKDLWSEEEKQEF
                     AKLDKMGVKVYQVDKAPFIEKVQPMYANFAKDNPALAPMLADIQAAK"
     gene            complement(1106..2407)
                     /locus_tag="ACHKTW_002485"
     CDS             complement(1106..2407)
                     /locus_tag="ACHKTW_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000386845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HFO6463490.1"
                     /translation="MDYWLPIIVLFGAFFFMLALGVPIVYAIGLSTLASISTQLDFNS
                     ALSVVSQKLASGLDSFTLLAIPFFILSGNIMNHGGIARRLINFARILGGRLPGSLAHC
                     NILANMLFGAISGSAVASAAAMGGVMHPQQVKEGYDPAFSTAVNVASAPTGLLIPPSN
                     TLIVYSLVSGGTSIAALFLAGYVPGILLGLALMVIAGIIAVRRGYPKPERPTLRQAGV
                     AIWMAIPSIFLIVLIMGGVLSGIFTPTEASAIAVIYTLFLALVLYREISVKDLPKIFL
                     ESVITTAIVLLLIGSSMGMSWAMSNADVPFLILDLLNTISDNPIIILLIINIILLIIG
                     TFMDMTPAVLIFTPIFLPVVTELGMDPIHFGIVMVLNMCIGICTPPVGSVLFVGCSVS
                     KLPINKIIKPMLPFYAVMVLVLAMVTYIPQISMALPRALGY"
     gene            complement(2450..2926)
                     /locus_tag="ACHKTW_002486"
     CDS             complement(2450..2926)
                     /locus_tag="ACHKTW_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012311688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFO6463491.1"
                     /translation="MRNIIRSINALLAALNITILAIIVACVTWQVAARFIFTSPSIFT
                     DELSRLLFICLGLFGGAYTAGQNRHLAIDLLPMMLKGKARRHLFLCIQIIVIIFATII
                     MVYGGGLLTMDTFDSGQTSPALGWQMGYIYMSIPISGVLIIIYTIDMVLTELKQPL"
     gene            complement(2947..5028)
                     /locus_tag="ACHKTW_002487"
     CDS             complement(2947..5028)
                     /locus_tag="ACHKTW_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005766390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HFO6463492.1"
                     /translation="MSQLDDTILDALTHVTFPKGFAQAEPAWVVTVDGVDYPLWQTDA
                     LVVGSGAAGLRAAVELKRRQQNVLIATAGLYMGTSACSGSDKQTLFTAATAGNGDNFT
                     KLAEALASGGAMDHDTAYVEAVGSLHTLGGLQYLGLELPEDRYGAILRYQTDHDEAGR
                     ATSCGPRTSRLMVKVLLEEVQRLAIPVLTSATVIKLLHQRDENGEDRVAGAILATGHR
                     AHNPWGLAIVTAPNVVLATGGPGELYRDSVYPHKCFGSLGLALEEGLTLTNLTESQFG
                     IGTPRSTFPWNLSGTYVQVIPYIYSVDAEGNEYNFLADYYRTTQELASNIFRKGYQWP
                     FHATRVMDFGSSLLDMAVAQEQQSGRQVFMDFNRNPEAVPGDLPFSLDRLDDDVRAYL
                     ENNDALAPSPIERLQRMNPLSISLYKMHGYDLTTQPLQFAMNNQHMNGGIEVDIWGQT
                     SLPGCFAVGEVAGTHGVTRPGGAALNAGQVFAVRLARFIGCTQKRNIDGDIAQLVAPT
                     LASIREIITQAHDNGTGMPLSVVREKIQARMSDHAGFICHADKVRRATRDALLLSEFV
                     QRHGLAIKHVGEVAELFMWRHMALTSAAVLTQLTHYIDAGGGSRGARIILDRDGNSIP
                     QTRNGFCDAWRFRSERTEDKKDKLLIHYCNGIFHVRETPVREFPIIRGIWFEKNWPGF
                     LNGTIYQPQDE"
     gene            complement(5025..6566)
                     /locus_tag="ACHKTW_002488"
     CDS             complement(5025..6566)
                     /locus_tag="ACHKTW_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HFO6463493.1"
                     /translation="MRKITTAEALAAQIQDGATIAISGNGGGMVEADHIMAAIEARFL
                     QTGHPRDLTLIHSLGIGDRDCKGTNRFAHAEMLKRIIAGHFTWSPKMQALVKNNTIEA
                     YCFPGGVIQALLREIGAGRPGLFTHVGLGSFVDPRNGGGKSNECTTDDLVELIEIDGE
                     TKLRYRPFKVDYAILRGTYADPRGNVSLEEEAIDMDSYSMALAAHNSGGKVFVQVRDV
                     LEAGAIEPRRVKLPGILVDGIVEHREQPQTYLGGYDLTISGQHRRLSSNDAIELVSHP
                     VRRLIARRAARELVAGASTNFGFGIPGGIPGVALREGVPYQSLWLSVEQGVHNGMMLD
                     DAFFGCARNADAIIPSLDQFEFYSGGGIDITFLGMGEMDQYGNVNVSHLNGNLIGPGG
                     FLEIAQNARKVVFCGTFDAKGSKIDVTPDGLHITQSGQIPKLVTQVEKITFSAAYAQQ
                     SGQEVLYITERAVFQLTAEGVELIEIAPGVEIERDILPYMAFRPIIKHPRLMESSLFT
                     PMEDA"
     gene            complement(6576..7352)
                     /locus_tag="ACHKTW_002489"
     CDS             complement(6576..7352)
                     /locus_tag="ACHKTW_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HFO6463494.1"
                     /translation="MSDQPVLFSRVAASCRLTLNREDKCHAINEEMIESLDHYLNEIE
                     NDTTLRLVELTATGDKFFCAGGDIKSWSAYSPLDMGRKWIKRGNDVFNRLRNIPQLTV
                     ANLNGHTIGGGIELALCCDIRIARPGAKFSNPEVMLGMVPGWMGIERVLNQVGPVVGR
                     QMLMLGKRLTAQEAQAANLIDEVVEKEQVESWMANQLAQLEKCGPVALAHIKQLILAL
                     ENKHADYPHQLLAGLMSATQDCQQATRAFAEKSSVSFHNQ"
     gene            complement(7362..8204)
                     /locus_tag="ACHKTW_002490"
     CDS             complement(7362..8204)
                     /locus_tag="ACHKTW_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFO6463495.1"
                     /translation="MRDLLQRPDLFSINTATLGYKTPLPAIIDACAARGIGAIAPWRR
                     ELQGEDLQQIAHQLAASNMSVSGLCRSTYYTAPTLAERKLAIDDNRRALDDAAVLNAA
                     CYMQVVGGLPTGTKDLYEAREQVKQGIRQLLPHSKDVGVPIALEPLHPMTAADRSCLC
                     TLRQALDWCDELDPDGEFGLGVAVDVYHVWWDPDLASQILRAGKRILAFHVSDWLVPT
                     TDLVNDRGMPGDGVINIPSIRRLVENAGFNGAIELEIFSPYWWQKDINSTLDISVDRI
                     AHYC"
     gene            complement(8214..9005)
                     /locus_tag="ACHKTW_002491"
     CDS             complement(8214..9005)
                     /locus_tag="ACHKTW_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase"
                     /protein_id="HFO6463496.1"
                     /translation="MKSTRPVAVITGAARGIGKGCALELARGGFNLLINDRPDADSVE
                     KLHITQQECLAEGVEVICFPADVGDLSLHEEMLDAAQNQWGRLDCLLNNAGISVKKRG
                     DLLDLEPDSFDQNIAINTRAPFFLAQAFSKRLLAQPKPEAELPHRSIIFVSSINAIML
                     AMNRGEYTIAKTAVSAAARLFAARLCNEQIGVYEVRPGLIKTDMTIPATAYYDELIAK
                     GLVPWGRWGYPADIASTVRAMAEGKLIYTCGQAVAIDGGLSMPRF"
     gene            9218..9865
                     /locus_tag="ACHKTW_002492"
     CDS             9218..9865
                     /locus_tag="ACHKTW_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFO6463497.1"
                     /translation="MYIDTSNVQSLKEKLLLCAVNEFAEYGYEGARVDNIVKAAGCSK
                     QTVYHHFGNKENLFIEVLEYTWNDIRQKEKALDFSDLPPQKAIEKIIDFTWDYYIANP
                     WFLKIVHSENQSKGVHYAKSQRLLEINHAHLQLMESLLDEGKKHNIFKPDIDPLQVNI
                     NIAALGGYYLINQHTLGLVYHISMVSPQALEARRKVIKETILSWLLVDPSSTAHE"
     gene            complement(9849..10487)
                     /gene="ytfB"
                     /locus_tag="ACHKTW_002493"
     CDS             complement(9849..10487)
                     /gene="ytfB"
                     /locus_tag="ACHKTW_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YtfB"
                     /protein_id="HFO6463498.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNAPVVTREAQLDIQSQSQPPTEEQLRAQLVTPQNDPDQVAPVAPEPIQ
                     EGQPEEQPQTTQTQPFQPDSGIDNQWRSYRVEPGKTMAQLFRDHGLPATDVYAMAQVE
                     GAGKPLSNLQNGQMVKIRQNASGVVTGLTIDTGNNQQVLFTRQPDGSFIRAR"
     gene            10706..11326
                     /gene="fklB"
                     /locus_tag="ACHKTW_002494"
     CDS             10706..11326
                     /gene="fklB"
                     /locus_tag="ACHKTW_002494"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFO6463499.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            11635..13047
                     /gene="cycA"
                     /locus_tag="ACHKTW_002495"
                     /gene_synonym="dagA"
     CDS             11635..13047
                     /gene="cycA"
                     /locus_tag="ACHKTW_002495"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HFO6463500.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHIQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(13092..13754)
                     /gene="ytfE"
                     /locus_tag="ACHKTW_002496"
     CDS             complement(13092..13754)
                     /gene="ytfE"
                     /locus_tag="ACHKTW_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HFO6463501.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(13862..14827)
                     /gene="ytfF"
                     /locus_tag="ACHKTW_002497"
     CDS             complement(13862..14827)
                     /gene="ytfF"
                     /locus_tag="ACHKTW_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFO6463502.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALICIGIGLASVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKYPMMWATAQALVTLPVSLIGYLVACYWLNIQ
                     TPDFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLITLSGIALLVIGVVIAVRAKPEKPLTESVSES"
     gene            complement(14935..15795)
                     /gene="qorB"
                     /locus_tag="ACHKTW_002498"
     CDS             complement(14935..15795)
                     /gene="qorB"
                     /locus_tag="ACHKTW_002498"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HFO6463503.1"
                     /translation="MIAITGATGQLGHYVIESLKKTVPVSQIVAIVRNPAKAQALAAQ
                     GITVRQADYGDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADTSPLGLADEHIETEKMLADSGIVYTLLRNGWYSENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYAAAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDNSKTLSKLIGRPTTTLAE
                     SVSHLFNVNN"
     gene            15884..16264
                     /gene="ytfH"
                     /locus_tag="ACHKTW_002499"
     CDS             15884..16264
                     /gene="ytfH"
                     /locus_tag="ACHKTW_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFO6463504.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            complement(16393..18336)
                     /gene="cpdB"
                     /locus_tag="ACHKTW_002500"
     CDS             complement(16393..18336)
                     /gene="cpdB"
                     /locus_tag="ACHKTW_002500"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008663 - 2',3'-cyclic-nucleotide
                     2'-phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HFO6463505.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKAGDIHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VVVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIT
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLTAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIDPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAGDKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            18526..19266
                     /gene="cysQ"
                     /locus_tag="ACHKTW_002501"
     CDS             18526..19266
                     /gene="cysQ"
                     /locus_tag="ACHKTW_002501"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HFO6463506.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLTPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKQIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            complement(19256..19813)
                     /gene="ytfJ"
                     /locus_tag="ACHKTW_002502"
     CDS             complement(19256..19813)
                     /gene="ytfJ"
                     /locus_tag="ACHKTW_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001498728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HFO6463507.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFETGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEKSSAVVVLDKDGRVQ
                     WAKDGALTQEEVQQVMDLLHKLINK"
     gene            20138..20344
                     /gene="ytfK"
                     /locus_tag="ACHKTW_002503"
     CDS             20138..20344
                     /gene="ytfK"
                     /locus_tag="ACHKTW_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HFO6463508.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDVGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            complement(20406..21749)
                     /gene="paeA"
                     /locus_tag="ACHKTW_002504"
     CDS             complement(20406..21749)
                     /gene="paeA"
                     /locus_tag="ACHKTW_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFO6463509.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANM
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(22072..22710)
                     /gene="msrA"
                     /locus_tag="ACHKTW_002505"
     CDS             complement(22072..22710)
                     /gene="msrA"
                     /locus_tag="ACHKTW_002505"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFO6463510.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDQGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRRITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            22916..24649
                     /gene="tamA"
                     /locus_tag="ACHKTW_002506"
     CDS             22916..24649
                     /gene="tamA"
                     /locus_tag="ACHKTW_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HFO6463511.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQTLDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQNVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFAGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            24646..28425
                     /gene="tamB"
                     /locus_tag="ACHKTW_002507"
     CDS             24646..28425
                     /gene="tamB"
                     /locus_tag="ACHKTW_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HFO6463512.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQPGVAVKAGNLHLAVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVAEVAQEEVVEPKIENPQPDEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVNTMLLKVSSIDGNTKLDALDIDS
                     NQGIVNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEFPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVSKIGETFGVSNLALDTQGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            28428..28769
                     /gene="ytfP"
                     /locus_tag="ACHKTW_002508"
     CDS             28428..28769
                     /gene="ytfP"
                     /locus_tag="ACHKTW_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HFO6463513.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
BASE COUNT         7086 a         7267 c         7426 g         7013 t
ORIGIN      
        1 accattggtc ggcgttttgc ttttgtagcg agcacttgca gatatcgata agggccacaa
       61 taagcagccc tacgaagata attacttagc cgcctgaata tcagccagca ttggggcaag
      121 ggctgggtta tccttagcga agtttgcgta cattggctgt actttctcga taaacggagc
      181 tttatctacc tggtagactt tcacgcccat tttatccagt tttgcaaatt cttgtttctc
      241 ttcctcgctc cacaagtctt tcatgtagct cattgattct ttagctgctt ttaatataac
      301 ttcctgattt tccgggctaa gtttatcaaa cgcagcattg ctcatcagaa ccacatcggg
      361 aaccatagtg tgttcgtcaa ggctgaagga ttttgccact tcaccgtgac gcatggtggt
      421 cagcgccatc acgctgtttt ctgccatatc gaccacaccc tgctgtaagg ctgtatacaa
      481 ctcgccgtaa tccagtggcg tcgggttacc gcccatgact ttgatcattt caactgcgcc
      541 agggcttggc tggacacgga ctttcatgcc tttcagatcg tctggtttca gtactggttt
      601 attgccatag aaactgcggg cgcctccatc ataaaaagtc agcccgaaat aacctttgct
      661 tttcgttgag tcgaggattt ttctcccgat atcgccctgc atgacctgat aataatgatc
      721 acgatcgcgg aacaaataag gcaggctaaa taatttatag gaattttcaa atgcttccat
      781 ttctgccgca ttggttttta ccagtggaat agcgccagaa cgaatcagct ccattgtttc
      841 tcgttgagta cccaatgttc catttggata aatacgaata gtaatatcac cgttagagta
      901 ctctttgctc tttttcgcaa agaactccat cgctttatga acaggatgag acttatcctg
      961 attatgactc agttttaatg tcactgccca ggactggctt gcggataaca taagaacagc
     1021 accgataatg ccagcggata cttttatttt catagatgtt ccttttctaa taaatatggc
     1081 ggcaggtgcc gctagtgaat gcgcatcagt aacccagtgc tcttggcaat gccatactga
     1141 tttgcggaat ataggtcacc attgccagca ccaataccat cacggcatag aatggcagca
     1201 taggtttgat aattttatta atcggtaatt tagatacact acaaccgaca aataacacac
     1261 tgcctacagg cggagtacaa ataccgatac acatgttcag caccatgaca ataccaaagt
     1321 gaatcgggtc catgcccagt tcggtgacaa ccggtaaaaa tatcggcgta aaaatcagta
     1381 ccgccggtgt catatccata aaagtgccga taatcaacag gataatattg ataataagca
     1441 ggattattat tgggttgtca gaaatagtgt taagcaaatc gagaatcagg aagggaacgt
     1501 cagcgtttga cattgcccat gacattccca tcgaagaacc aattaataac aagacaattg
     1561 cagtggtaat aacggattcc agaaatattt taggtaaatc tttaactgag atttcacgat
     1621 aaagaaccag tgcaaggaaa agggtataaa ttacagcaat tgcggaggct tcggttggag
     1681 taaaaatacc gcttagtacg ccacccataa ttaacacaat caggaaaata ctggggatag
     1741 ccatccatat cgcaacgcca gcctggcgca aagtcggacg ttctggtttt ggataaccgc
     1801 gacgtacagc gataataccg gctattacca tcagtgccag acccagcaaa atccccggaa
     1861 cataacccgc caggaacagt gcggcaatag aagtaccacc actgaccagt gaatagacga
     1921 ttaacgtatt acttggtgga ataagcaaac ccgtcggggc agaggcaacg ttaaccgcag
     1981 tactgaatgc cgggtcgtag ccctctttaa cctgttgcgg atgcatgacg ccgcccatcg
     2041 cagccgccga agccacggct gaaccagaaa ttgccccgaa cagcatattg gcgagaatat
     2101 tacagtgggc aagtgagcct ggcagacgac cgccgagaat acgggcaaag ttgattaatc
     2161 gtcgcgctat tcctccatga ttcataatgt tgccagacaa gataaaaaat gggattgcca
     2221 acaacgtaaa actatctaac ccggaggcta acttttggga aaccacactc aatgctgagt
     2281 tgaaatcgag ttgtgtggaa attgatgcca gtgtcgataa accaatggca taaacaatag
     2341 gtacacctaa tgccaacata aaaaagaagg caccaaataa cacaataatc ggtaaccagt
     2401 aatccataat ttctccattc ctactatggt tggttgttgt ttgttatctt tagagtggtt
     2461 gtttaagttc ggttaaaacc atatctatgg tgtatataat aataagtaca ccactaatcg
     2521 ggatgctcat atagatatat cccatttgcc agcctaaagc gggcgaggtc tgacctgaat
     2581 cgaatgtatc catcgttaat agcccaccac catataccat aatgatggtt gcgaagataa
     2641 taacgataat ctgaatacac aaaaataaat gacgtcgggc ttttcctttc agcatcatag
     2701 gtagcaggtc aatcgccaga tgccggtttt gccctgcggt gtatgcccca ccaaaaagtc
     2761 caagacaaat aaagagcaaa cggctgagtt catctgtaaa aatactgggg ctagtaaata
     2821 taaatcgtgc agccacctgc cacgtcacac atgccacgat gatcgccaaa atggtaatgt
     2881 tcaatgctgc aagcagagca ttaatagatc taattatatt tcgcatagtg attaaccttt
     2941 gcaatatcat tcatcctgtg gctggtaaat tgtcccatta agaaaaccag gccagttttt
     3001 ttcaaaccaa atacctcgga taataggaaa ctcacgaaca ggtgtttctc tgacgtgaaa
     3061 gataccgttg caataatgaa taagtagttt atctttttta tcctcagtac gctctgaacg
     3121 aaagcgccag gcatcacaaa aaccatttcg tgtttgcgga atagaatttc catccctgtc
     3181 gagaataata cgtgctcccc gactaccacc acctgcgtca atataatgcg ttaattgcgt
     3241 taatacggcg gcagaagtca gtgccatatg ccgccacata aacagctcgg caacttcacc
     3301 cacatgtttg atagccaatc catgccgttg gacaaattcg ctcaatagca gggcatcgcg
     3361 agtggcgcgt cggactttat cggcatggca aataaatccc gcatggtcag acattcgtgc
     3421 ctgaattttt tctctcacaa ccgacaacgg catcccggtc ccgttatcgt gcgcctgagt
     3481 aatgatctct cttatagaag ccagtgtagg agctaccagc tgtgctatat ctccatcaat
     3541 attccgcttt tgcgtgcaac caataaaacg tgccagacga acagcaaaaa cctgcccggc
     3601 attcaatgct gcaccaccag ggcgagtgac gccgtgtgtg ccagcaactt cccccacggc
     3661 gaaacagccg ggcagggagg tttgtcccca gatatccact tcaatgccgc cattcatatg
     3721 ctgattattc atggcaaact gcaaaggctg tgtggtgaga tcgtaaccgt gcatcttata
     3781 cagcgagata gacagcggat tcattcgttg cagccgttcg atgggcgatg gtgccagagc
     3841 gtcgttattt tccagatacg cgcgaacgtc gtcgtccagt cgatctaatg agaacggcag
     3901 gtcacccggc accgcttcag gattgcgatt gaaatccata aatacctgac gccctgattg
     3961 ctgctcttgc gctactgcca tatctaacaa gctggagcca aaatccatca cccgcgtggc
     4021 gtggaacggc cactgatagc ctttacggaa aatgtttgaa gccagctcct gggtggtgcg
     4081 atagtaatcc gcgaggaagt tatactcgtt accctcagca tccacggaat agatatacgg
     4141 gatcacctgt acataggtgc cggataaatt ccacggaaac gtgctacgcg gcgtgcctat
     4201 accaaactgg ctttcggtca gattggttag cgtcagacct tcctccagcg ccagccccag
     4261 cgagccgaag catttatgtg gataaacact gtcgcgataa agctcgccag gccctcctgt
     4321 tgccagtacc acattgggcg cagtcacaat tgccagcccc catgggttat gggcgcgatg
     4381 accggtcgcg aggattgccc ctgccacgcg gtcttcgccg ttttcgtcac gctgatgcag
     4441 cagtttaatc actgttgcac tggtcaacac tggaatggcg aggcgctgta cttcttccaa
     4501 cagcactttc accattaacc ttgaggtacg cggcccacac gaggttgcac gcccggcttc
     4561 gtcatggtcg gtttgataac gaagaatcgc gccatagcga tcttccggta attccagacc
     4621 aagatattgc aacccgccta gagtgtgcag agaacctacc gcttcgacat aagcggtgtc
     4681 gtgatccatc gccccaccgc tcgccagtgc ctctgccagt ttggtgaagt tgtcgccgtt
     4741 gcccgcggta gcggcggtaa atagtgtctg tttatcggaa ccagaacatg ccgacgtccc
     4801 catatataac ccggcggtag cgatcagcac attttgctgg cggcgtttaa gttcaactgc
     4861 cgcacgtaat cctgctgcac cactgccgac taccagggca tctgtttgcc agagtgggta
     4921 atcaacaccg tctaccgtga caacccatgc gggctctgcc tgtgcaaaac cctttgggaa
     4981 agtaacgtgc gtcagcgcat caaggatggt gtcatcaagc tgactcatgc atcctccatc
     5041 ggcgtaaaca ggctactttc catcaggcgt gggtgcttga taattggacg gaaagccata
     5101 tacggcagaa tgtcgcgctc aatctccaca cccggcgcga tttcaattaa ttcaacgcct
     5161 tctgccgtta actggaatac tgcgcgttca gtgatataca acacttcctg accactttgc
     5221 tgtgcgtagg cggcgctaaa agtgattttt tccacctggg taaccagttt agggatttga
     5281 cctgactggg tgatatgcaa gccatctggc gttacatcaa tcttgctacc tttggcgtcg
     5341 aacgtgccgc agaacaccac tttacgggcg ttttgcgcaa tttcgagaaa tccgccgggg
     5401 ccaatcagat tgccattgag atgggagacg ttgacgttac cgtactgatc catctctccc
     5461 atgccgagga aggtgatatc gatccctccg ccactgtaga actcaaattg atcgagtgat
     5521 ggaataatgg catcggcgtt gcgggcgcag ccgaaaaacg catcatccag catcataccg
     5581 ttatgcacgc cttgctcgac gctcagccat aaactttggt aaggcacgcc ttcgcgcagc
     5641 gctacgcctg gaataccccc aggaatacca aagccaaagt tggttgaagc gccagctacc
     5701 agttcgcgtg ccgcccgacg ggcaatcagg cgacgtaccg gatgactaac cagttcaatg
     5761 gcgtcgttag aactcagacg gcgatgttgg ccgctgatgg tcaggtcgta accgccaaga
     5821 taggtttgtg gttgttcgcg gtgttcaacg atgccatcaa ccagaatgcc cggtaatttg
     5881 acccggcgtg gttcaatcgc gccagcttcc agcacatcgc gtacctgtac gaacactttg
     5941 ccgccgctgt tgtgtgctgc cagcgccatg gaatagctat ccatatcaat cgcttcttct
     6001 tcaaggctga cgttgcctcg aggatcggca taagtgccac gcaaaatcgc gtaatccact
     6061 ttgaaagggc gataacgaag tttggtttca ccgtcgattt caatcagttc taccaggtcg
     6121 tcggtagtgc attcattcga cttaccgccg ccattgcgtg gatcaacaaa cgatcccagc
     6181 ccaacgtggg taaaaagccc cggacgtccg gcgccgatct cccgtagcag tgcctgaatc
     6241 acgccaccag gaaagcagta ggcttcaatc gtgttatttt ttaccagcgc ctgcattttg
     6301 ggcgaccagg taaagtgccc ggcaataatg cgtttgagca tctctgcgtg cgcaaaacgg
     6361 ttagtgcctt tgcagtcgcg atcgccaata cccagcgagt ggattagcgt caggtcgcgc
     6421 gggtgtccgg tttgcaggaa acgcgcttca atggcggcca taatatggtc agcttccacc
     6481 atgccgccgc cgttaccgct aatggcaatg gttgcgccgt cctgaatttg cgccgccagt
     6541 gcctcggcgg ttgttatctt acgcatacaa tttccttatt gattatggaa gctcacgcta
     6601 cttttttcgg caaaggcgcg cgtcgcctgc tggcaatcct gagtggcaga cattaatccg
     6661 gccagtagct ggtgcgggta atcggcatgt ttattttcca gtgccagaat cagttgctta
     6721 atatgcgcca gtgccaccgg accgcatttt tccagctgcg ctaactggtt cgccatccag
     6781 ctttccacct gttctttttc taccacttca tcaattaaat tcgccgcctg ggcttcctgc
     6841 gccgtcaggc gtttacccag catcagcatc tggcgaccga ccaccggacc aacctgatta
     6901 agcacacgtt caatgcccat ccagccaggc accatgccga gcatcacttc cgggttagag
     6961 aatttcgcgc cggggcgggc aatacgaata tcgcagcaca aggccagttc aataccaccg
     7021 ccgatggtat gaccgttgag attcgcaacg gttaattggg gtatgtttcg caggcgatta
     7081 aaaacgtcat taccgcgttt aatccatttt ctgcccatat ctaatggcga ataggccgac
     7141 cacgatttaa tatcgccgcc cgcacaaaag aatttatcgc cggttgccgt taattcgact
     7201 aatcgtaatg tggtgtcgtt ttcaatttca tttaaataat ggtcgagaga ttcgatcatc
     7261 tcttcgttaa tagcatgaca tttatcttca cggttcaatg tcaggcggca ggaagccgcc
     7321 actctgctga acagaacggg ttggtcactc atgatcattc cttaacaata atgcgcgatg
     7381 cgatcgacgc taatatccag cgtgctgtta atatctttct gccaccagta aggcgagaat
     7441 atttccagtt caatagcacc attaaatccg gcgttttcaa ccagccgacg aattgacgga
     7501 atattgatga cgccatctcc cggcatacca cggtcattga ccagatcagt ggtcggcact
     7561 aaccagtcgg aaacatgaaa cgcgaggatg cgttttccgg cacgcaggat ctgactggcg
     7621 agatccggat cccaccagac gtgataaaca tccaccgcca cgccgaggcc aaactcacca
     7681 tcgggatcga gttcatcaca ccagtccagc gcctggcgta acgtgcacag gcaagagcga
     7741 tcggcagctg tcatcgggtg cagtggctcc agcgcaattg gcacgccgac atctttcgaa
     7801 tgtggcagca actggcgaat cccttgtttt acttgttccc gcgcttcgta cagatctttt
     7861 gtgcccgttg gcagaccgcc aaccacctgc atataacagg cggcgttaag caccgctgcg
     7921 tcgtccagcg cccggcggtt atcatcaatc gccagtttgc gttccgccag tgtcggcgcg
     7981 gtgtagtaag tgctacggca caaaccggaa acgctcatgt tactggcggc aagctggtgg
     8041 gcgatttgct ggagatcttc accttgcaac tccctgcgcc agggagcaat cgcgccaata
     8101 ccacgcgcgg cgcaggcatc aataatggcg ggtagcggcg ttttatagcc cagtgttgcg
     8161 gtattgatac tgaataaatc cgggcgttgt aacagatctc tcatgattca gcctcagaag
     8221 cgcggcatac ttaagccgcc gtcgatggcg accgcctgac cacaggtgta aatcagttta
     8281 ccttccgcca tcgcgcggac agtggaagca atatcagccg gataacccca acgtccccac
     8341 ggcaccagac ctttggcaat cagttcgtcg taataagccg ttgccggaat agtcatatcg
     8401 gttttgatga gaccagggcg cacttcataa acgccaattt gttcattaca aaggcgagca
     8461 gcaaacagac gagcagcggc agaaacggcg gttttggcaa tggtgtattc gccgcgattc
     8521 atcgccagca tgatggcgtt gatggaactg acgaaaataa tcgagcggtg cggcagctcc
     8581 gcttccggtt tcggctgtgc cagtaagcgc ttactgaatg cctgagcaag gaagaacggc
     8641 gcgcgggtgt taatggcaat gttttgatcg aagctgtctg gttcgaggtc gaggaggtca
     8701 ccgcgttttt tcactgaaat ccccgcgtta ttgagcagac agtccagccg cccccactgg
     8761 ttttgcgccg cgtcgagcat ctcttcatgc aaggagagat cgccgacatc ggcagggaag
     8821 caaatgactt ccacgccttc tgccaaacat tcttgttgtg tgatatgcag tttttctacg
     8881 ctgtcggcat cgggacgatc gttaatcagc aggttaaatc cgccacgagc aagctccagc
     8941 gcacagccct ttccaatacc acgagcggct ccggtaatca cggcaacagg acgagtggat
     9001 ttcatacaat agctctcttt ttttgaatat tgttttctaa gtgacaatta gcttgcgacg
     9061 tgccgcaaga aagatcaacc atctggttgg ttgatatgcg atagtgatca cagtacattt
     9121 gataacttga tgaaagtaca agtttttatc ctttttactg gagcttctaa ttaatcgggt
     9181 taagatagtt atcttgaaga ggtcgtgggg aagcgttatg tatattgata ccagcaacgt
     9241 acagagcctg aaggaaaaat tgctgctatg tgcggttaat gaatttgccg aatatggtta
     9301 tgaaggggcc agagtagata atattgtaaa ggcagcaggg tgtagtaaac agacggttta
     9361 tcaccacttt ggtaataaag aaaatttatt tatcgaagtg ctggaatata cctggaacga
     9421 tattcgacaa aaagaaaagg cccttgattt ctcagatctt ccgccacaaa aagctattga
     9481 gaaaattatt gattttacat gggattatta tattgctaac ccctggtttt taaaaattgt
     9541 gcatagtgaa aatcaaagta aaggtgtgca ttatgcaaaa tcacagcgtc tcttagaaat
     9601 taaccatgcg cacttgcagt taatggaatc cttattggat gaagggaaga aacacaatat
     9661 ttttaagcca gatatcgacc cattgcaggt gaatattaat attgctgcgc ttgggggata
     9721 ttatttgatc aaccagcata cgcttggcct ggtttatcat atcagtatgg tttcccccca
     9781 ggcactggaa gccagacgta aggtcatcaa agagacaatc cttagctggc ttttggttga
     9841 cccttcatct accgcgcacg aataaaactg ccatccggct ggcgggtaaa caggacctgt
     9901 tgattattcc ccgtatcaat ggttaagccc gtcaccacgc cgctggcgtt ctggcggatt
     9961 ttcaccattt gcccgttttg caaattactc agcggcttac ccgcgccttc tacctgcgcc
    10021 attgcataga catcggtagc gggcagtccg tggtcgcgga acagctgcgc catcgtttta
    10081 ccgggttcga cacggtaaga gcgccattga ttatcaattc cgctatctgg ctggaatggt
    10141 tgcgtttggg tagtttgcgg ctgctcttct ggctggcctt cctgtatagg ttctggagcg
    10201 accggagcca cctgatcagg atcattttgt ggggtgacta attgcgcccg cagctgttct
    10261 tctgtcggcg gttgtgattg cgactgaata tctaactgcg cttcacgcgt caccaccggc
    10321 gcgttgggcg tatcatcaga tggaagcaga aatccgacca ccaatacaat ggcggcaata
    10381 atgatccccc gacgatgcat cggcggcagc gggtccatga agcgaaaatt gtccggcgcg
    10441 tgccagactt tctccagggt tggttttagt tcaaagcgcc cgggcatggt ttcctcctgc
    10501 tccgcgtctt gttcctcaat catagcctat gaataagcta acgctatgat gtccgtggta
    10561 aacccgcctt tattatattc atacgggata ttgctattgt ttctttttcc ccgggatttg
    10621 tcatcattcc tgcgacaaag ttttacccaa agaagtgtgg ctgatatgct gcccgctact
    10681 tttataccct aagaaaggaa atacgatgac caccccaact tttgacacca tcgaagcgca
    10741 agcaagctac ggcattggtt tgcaggtagg gcaacaactg agtgaatctg gcctggaagg
    10801 gctgctgcca gaagcactgg ttgcaggtat tgccgatgcg ctggaaggca aacatccggc
    10861 tgttccggtt gatgtggtgc atcgcgcgct gcgtgaaatc cacgagcgcg ccgatgccgt
    10921 tcgtcgtcag cgtttccagg cgatggctgc tgaaggtgtg aaatacctgg aagaaaacgc
    10981 caaaaaagaa ggtgtgaata gcaccgaatc tggcctgcaa ttccgcgtga tcaaccaggg
    11041 tgaaggcgca attccggcac gtaccgaccg cgttcgtgtt cattacaccg gtaaactgat
    11101 cgacggcacc gtgtttgaca gctccgttgc tcgtggtgaa cccgctgaat ttccggttaa
    11161 tggcgtgatc cctggctgga ttgaagcact gactctgatg ccggtaggtt ctaaatggga
    11221 actgactatc ccgcaggaac tggcatatgg cgagcgcggc gcaggcgcat ccatccctcc
    11281 gttcagcacc ctggtgtttg aagtcgaact gctggaaatc ctctaagcag cgcattctgt
    11341 tcccctcgaa ccagagggga gcaggcattc agcaataaac ccttcagttt gccaaacggc
    11401 gctattttgt gttgcaaaga ccccgtaagc gtgtattttt gtgagctgtt tcgcgttatc
    11461 accgtgatat gacactcact ttaaacataa aattaacatt agatctaaat cttagtattc
    11521 atcccgcgta ttgttaccta atatcgatga gtcccgatac agattcgtcg tatcatagac
    11581 tgactaaagg ccgtagagcc tgaacaacac agacaggtac aggaagaaaa aaacatggta
    11641 gatcaggtaa aagtcgttgc cgatgatcag gctccggctg aacagtcgct acggcgcaat
    11701 ctcacaaacc gacatattca gcttattgcc attggcggtg ccattggtac ggggttgttt
    11761 atggggtctg gcaaaacgat tagccttgcc gggccgtcga tcattttcgt ttatatgatc
    11821 attggtttta tgctcttttt cgtgatgcgg gcaatggggg aattgctgct ttcgaatctg
    11881 gaatacaaat cttttagtga cttcgcttcc gatttactcg ggccgtgggc aggatatttc
    11941 actggctgga cttactggtt ctgctgggtt gtaaccggta tggcagacgt ggtggcgatc
    12001 acggcttatg ctcagttctg gttccccgat ctctccgact gggtcgcctc gctggcggtg
    12061 atagtgctgc tgctgacgct caaccttgcc accgtgaaaa tgttcggtga gatggagttc
    12121 tggtttgcga tgatcaaaat cgtcgccatc gtgtcgctga ttgtcgtcgg cctggtcatg
    12181 gtggcgatgc actttcagtc accgaccggt gtggaagcgt cattcgcgca tttgtggaat
    12241 gacggcggct ggttcccgaa aggtttaagt ggcttctttg ccggattcca gatagcggtt
    12301 ttcgctttcg tggggattga gctggtaggt acaacagctg cggaaaccaa agatccggag
    12361 aaatcactgc cacgcgcgat taactccatt ccgatccgta tcattatgtt ctacgtcttc
    12421 gcgctgattg tgattatgtc cgtgacgccg tggagttcgg tagtcccgga gaagagcccg
    12481 tttgttgaac tgtttgtgtt ggtaggtttg cctgcggctg ccagcgtgat caactttgtg
    12541 gtgctgacct ctgcggcgtc ttccgctaac agcggcgtct tctctaccag ccgtatgctg
    12601 tttggtctgg cgcaggaagg tgtggcaccg aaagcgttcg ctaaactttc taagcgcgca
    12661 gtacccgcga aagggttgac cttctcgtgt atctgtctgc tcggcggcgt ggtgatgctg
    12721 tatgtgaatc ccagcgtgat tggcgcgttc acgatgatca cgaccgtttc cgcgattctg
    12781 tttatgttcg tctggacgat tatcctttgc tcgtacctgg tgtaccgcaa acagcgtcct
    12841 catctgcatg agaagtcgat ctataagatg ccgctcggca agctgatgtg ctgggtatgt
    12901 atggcgttct ttgtgttcgt ggtcgtgttg ctgacactgg aagatgacac tcgccaggcg
    12961 ctgttggtca ccccgctgtg gtttatcgca ctggggctgg gctggttgtt tattggtaag
    13021 aagcgggctg ctgaactgcg gaaataaccg cattatcatg ctggatggcg caatgccatc
    13081 cagcttttag attactcacc cgccagcgcg cgtgggaaca gtacattgtt ttccaagctg
    13141 atgtgatcca tcaggtcatc aatcagttca ttaatgccgt tatacatcgc tttccaggtg
    13201 gtgcaggctt ctggcggcgg tgtgacgtta ttggtagtgt gtttgatcac ttccagcagt
    13261 tcgcccgctt catcgtgctc gctttccatt acgctgattg gccccattgc ctggctgccc
    13321 atgccttgtt tgatcatcgg gaagaggatc tgctcttctt tcatcatgtg gctggaaagc
    13381 tcttcatgca gcatagtcag gtattttgtc agcccttttg gcacgctcgg tttgtcggcg
    13441 tgaacgcgct cgactttagt cgcttgcagg atcagctccg gcagctgctc gcggtgacga
    13501 tcgtggtagc gcacgatgat atggtcaatg atttctgcca gcggggcgct acgccagtct
    13561 ttctcaatcg gttgttcagc aagctttgcc agttcagctt caatgacctc aacatccagt
    13621 tctttacgtg ccgccgcgcg cgccagcgtc tgcttaccgc cacagcagta atccatatca
    13681 tatttacgaa acagagccga agcgcgaggg atagagagcg ccagttcacc taaaggttgg
    13741 tcgcgataag ccatagctga tacctcattc ttaataatat aagatgtatt ttaaatgcat
    13801 ctttaaggca aaagctataa cccttacgta gcagaggctt atttaactca ctgccagcgt
    13861 gtcaactttc tgagacagat tcagttaaag gtttttctgg ttttgctctg accgcaatga
    13921 ccacgccaat cactaacagc gcgataccgc tcagcgttat tagcggcggc atttgctggc
    13981 ggagtaaaaa ggtgtacagc aaacctgcca gcgtttcgaa aacaatcagc ggcccgagaa
    14041 tcactgtcgg taatcgctgg ctggcgacgt tccagcagag tgcgccaacc catgagcaaa
    14101 gcacggctat cgcaaccatc agactaataa acaccagcgg acgggggcca aaaggtaagg
    14161 agaagtccgg cgtttgtata ttcagccagt aacaggcgac gagatagccg atgagagaaa
    14221 ccggcagcgt gaccagcgcc tgcgccgtcg cccacatcat cggatatttg tcgggatttt
    14281 cccgcagcca gcgggcgttg cgcagggcat accatgccca gcagaccacg gaaactaacg
    14341 ctagcacgat gccagaggta taacgtgccc agtcaaaatc ggggagtccg tggtttaact
    14401 cagcaatatt cacactcgcc aggccgatgc caatacaaat cagtgccggg gcgagttttc
    14461 cccacgcgag tttgccgtcg cgctggctat aaagcagatt ggcaaagaca ggaatgacca
    14521 ccggcagggt gccgataatc atcgtggaaa caggcgcgcc agtacgttga atggcactgg
    14581 caaggcagaa gtaataaatg aggttgccca tcatagtgag catcaaggcg gtaagccagt
    14641 cccgacgcgc caactgacgc agacgcacgc gtcccagcca ggcaatgggc agcgcaatta
    14701 accctaacgc cagataacgc cccatcgact gcaacatcgc cgggtattcc ggtacgatca
    14761 acggcccgac aaaaataagc ccccacatca accctgctaa cagggcgtac agcacgccgc
    14821 taatcattac tggcatccat tggtctgtca gaagaaagtt caggaagcag acatgattgc
    14881 tcccggcatc ccgtcattat tagagaggat gcgggaggat gccactttaa ttaactagtt
    14941 attaacatta aaaagatggc ttacgctttc ggctaacgtt gtcgttgggc ggccaatcaa
    15001 tttgctaagc gttttgctgt tatcaaacag accacctttc gatgcgccaa cgtcagaatc
    15061 cgccagcata tccgccagtc cgtcgggcag tccgacgctt ttcagtgccg cggcgaaatc
    15121 ggcttcgctc agattttgat aggtaacctg tttgccgctc tgtttggtca gctctgccgc
    15181 taactgtgtc aatgtccagg cactatcgcc cgccagttcg taaaccttgc cttcgtgacc
    15241 ggcttcgcta atcacgcgtg ccgcagctgc cgcataatct gcccgcgttg ctgaggcgat
    15301 tttgccatcg cccgccgcac cgataaatac gccgtgttcc agtgctgccg gggcgctggc
    15361 gaggtagttt tcgctgtacc agccgttgcg cagcagggtg taaacgatgc cagaatcagc
    15421 caacattttc tccgtctcga tgtgctcatc ggcgaggccg agcggggagg tatctgcatg
    15481 tagcaggctg gtataagcga taaatttcac accagccgcc tttgcggcat taataacatt
    15541 acgatgctgc ggggcacgtt gacccacttc gctggaagag atcagcagta gtttttccac
    15601 tccctgaagt gcagatgtca gtgcggcttc atcgccgtag tcagcctgac gcacggtaat
    15661 gccttgtgct gccagagcct gggcttttgc cggattacga acgatagcca ctatttggct
    15721 gacaggaacc gttttcttca aggattcaat aacatagtga ccaagttggc cagtggcacc
    15781 agtaatagcg atcatgggaa gtctccatcg ttttgtcttg tgtttaaata cgctaacacc
    15841 taaacttact tttagtaagt acgcacaaaa aggtaagtat gaaatgagtc aggttagcct
    15901 gtcgcaacaa ctgaaagagg gtaatctctt tgcggaacag tgcccgtcgc gcgaggtgtt
    15961 gaaacacgtc accagccgtt ggggggtgtt gattctggtg gcgctacgcg aaggtactca
    16021 tcgctttagc gacctgcggc gcaaaatcgg cggggtgagc gaaaagatgc ttgcgcagtc
    16081 gttacaggcg ttagaacagg atggttttct taaccgtatc gcgtatccgg tggtgccgcc
    16141 gcatgtggaa tatagcctca cgccgctggg cgagcaggtg agcgaaaagg ttgctgcact
    16201 ggcggactgg attgagttga atttgcccga ggtgttggcg gtgcgggatg aacgtgcggc
    16261 ataactgccg gatgcgctga gcttatctgg tctacggtgc atggcttgta gggcggataa
    16321 gatgcgccag catcgcatcc gccaataaat gccggatgcg gttgatgatt tataggccga
    16381 aaaagaagtg ttttacttac tcaaatccac ctgataaatc gcaaacccga tatcatcggt
    16441 cgcgacttta ttcatcggat attgcccttt ctctttaata aacgctgcgg ctttatctga
    16501 cggagaagtt tcgaaacgga tatccagttt cttatcgcca gctatcggtg ctaaacgcca
    16561 gttgttatct gccgccgggt gaatttcccc cgcacgtttc gactcatcag caatccacgc
    16621 tgccagcacc gagcggttct catccggtga agcaaaagcg atatggctgt cgcccgtacc
    16681 ggcaaatttg ccgccgtaag cgcgatagtt attggtggca acgaggaaca tggcgttcgg
    16741 atcaatcggc ttgccattaa aggtcaggtt cttaatcctt tccgcattgg cattaatcat
    16801 ctggcactcg ccgtcataac gggcaggctg ggtaacatca atctgataat tcacaccatc
    16861 aatcacatca aagttatagg tgcggaaacc atcccagttg atgagtgatt gcggtttcgt
    16921 gctgttagga tcaatctggt taaactgtcc ggcggagcac tccagccact ctttcacctc
    16981 tttaccgctg gctttcacta caatcagcgt attggggtag agataaagat cggcggcatt
    17041 acggaaggtc aactggcctt tttccacctc cacatagctt gccgggtcat ttttgcgacc
    17101 accaacttta aacggtgcgg cagctgaaag caccggcagt tttgccagat ccggatcgcc
    17161 ctgaatgtaa tgttcgacat acgctttttg cgcgttgttc actacttgca cggtcggatc
    17221 gtcctgcacc agcgccagat agctatacat attgtcggca gatttaccga ttggcttgct
    17281 gacgaactgg cgtgtggcat cgtgatcggc tttgagtgtt tctaccagct tgctgtcttc
    17341 cgccgtgagg gattttttat tggcgatgtc gtaaatcggc cgagcttccg ctttcgcctg
    17401 cgtcacctgc catttaccgc tgtcattact gagttgtaag tcgaccaccc caagatgatc
    17461 gccccacatg cccggcatta ccgccggaac accattcagc gtgcctttgg tgatatcagc
    17521 cccttcgata tcagcaaaat ctttacccgg gaaaacggcg tgagcatgac caaacataat
    17581 ggcgttaacg cccggaattt cactgaggta ataaactgag ttttccgcca tcactttata
    17641 cggatcggca gacagcccgg aatgtgccag aacgacaacg acatcggcac ctttctcgcg
    17701 catttcaggc acgtatttgc gcacggtttc ggtaatatca ttcaccgtca ctttcccgga
    17761 taaattagct ttatcccagc ccatgatttg cggcggtacg acgccaatat agccaatctt
    17821 cagcgtctgt ttttttccgt ctttatcgac cacttcggtg tctttaatta aatacggtgt
    17881 aaacattggc tgtttggttc tggcgtcaat gacgttggca tttacataag ggaatttcgc
    17941 tcccgccagc gcatttttca ggtaatccag accgtagtta aactcatggt tgccaagcgt
    18001 gccgacggta tagtccagcg tatttaatgc cttatacact gggtgaatat cacctgcttt
    18061 taatcctttc gccgacatgt aatcggccag cggactcccc tgaatcaaat cgccgttatc
    18121 gaccagtacg ctgtttttca cttcattgcg ggcatcgtta atcaggcttg ccgtacgtac
    18181 cagtccgaat ttttccgtgg cggtgtcttt gtaataatcg aaatccatca tgttgctatg
    18241 cagatcagtg gtttccatga tacgcagatc gaccgtcgct gcattcacac tggcggcaat
    18301 cagcgtggcc aggagcgttg cgctaaactt aatcatcagg gacatccttt tatcatcggg
    18361 aatacgaaag aaaagggaga ataaacgtct tacttataga acagtgaaga atgccacaat
    18421 tttacgcttt gaaaatgatg acactatcac agttggcgca ttcattaacg atagggtata
    18481 agtaaaacaa taagttaaca ccgctcacag agacgaggtg gagaaatgtt agatcaagta
    18541 tgccagcttg cacggaatgc aggcgatgcc attatgcagg tctacgacgg gacgaaaccg
    18601 atggacgtcg tcagcaaagc ggacaattct ccggtaacgg cagcggatat tgccgctcac
    18661 accgttatca tggacggttt acgtacgctg acaccggata ttccggtcct ttctgaagaa
    18721 gatcctcccg gttgggaagt ccgtcagcac tggcagcgtt actggctggt agacccgctg
    18781 gatggtacta aagagtttat taaacgcaat ggcgaattca ccgttaacat tgcgctcatt
    18841 gaccatggca aaccgatttt aggtgtggtg tatgcgccgg taatgaacgt aatgtacagc
    18901 gcggcagaag gcaaggcctg gaaagaagag tgcggtgtgc gcaagcagat tcaggtccgc
    18961 gatgcgcgcc cgccgctggt ggttatcagc cgttcccatg cagatgcgga gctgaaagag
    19021 tatctgcaac agcttggcga acatcagacc acgtccatcg gctcttcgct gaaattctgc
    19081 ctggtggcgg aaggacaggc gcagctgtac ccgcgcttcg gaccaacgaa tatttgggac
    19141 accgccgctg gacatgctgt ggccgcagct gccggagcgc acgttcacga ctggcagggt
    19201 aaaccgctgg attacactcc gcgcgagtcg ttcctgaatc ctgggttcag agtgtctatt
    19261 tattaattaa tttatgcagc aggtccatca cttgctgcac ctcttcctga gtaagcgccc
    19321 cgtctttggc ccattgcacg cgaccgtctt tatccagcac cactacagcg gaactttttt
    19381 catccagctg ccaggcaccg cgtgcgacgc cattgctatc aacaataaac tgcgaccagg
    19441 gataaagctt tttattactc tccagactgc tgcgcacaaa catgccggaa cccggaattg
    19501 cgtcgtcggt gttaacaatg gtggtggtct ggtaacgatc gtgcggtaac ttcgctgatt
    19561 taatcgcttc aatcagcgtc gcgtttttct cttttgcaga ggtgcgacca gcaatatgtt
    19621 gcagtactcg cacttttccc actaactgcg cgctgttcca ggttttgtag ctaaactgat
    19681 ctttatcaag caccaactcg ccacgatcgg tgatgccaat cggcggcact cgctgaccgg
    19741 tttcgaactg atgtgcggaa gccatcatcg gcaacagcag gcaggtgagt gccagaatct
    19801 tgcgtagggt catggtgttt ccttcttatg atatgcaggt gatccgacca cttgggtctg
    19861 agttttaatc atatgtgcta tttatcgaat ttcccgcaag tgtgatgcca gtttgcggtc
    19921 aagcgcacaa atcatatgaa aaatgaatgc ttatactgaa gaccgcgctt cggtaaaaag
    19981 ataattctga ataattgtaa cctttaggta aaaaaagtta tacgcggtgg aaacattgcc
    20041 cggatagtct atagtcacta ggcattaaaa tttgcgcctc ataatagttg ggccgattgt
    20101 ggcaccgcac aggcgtaata ctcagcagga gataacaatg aaaattttcc aacggtacaa
    20161 cccacttcag gtggcgaagt acgtaaagat cctgttccgt ggacggttat acatcaagga
    20221 cgttggcgct tttgaatttg ataagggtaa gattcttatc ccaaaagtga aggataagct
    20281 gcatttgtca gtgatgtccg aagttaaccg tcaggttatg cgtctgcaaa cagagatggc
    20341 ttaaccaaag tgctatgcag taataagacg gctcctgatt caggagccgt tgatgtttct
    20401 gggggttacg cgacgctttc ttctttatct ttagcgtcag gcagttttgg cgaaagggcg
    20461 gtggccttgc tgtcgatccg ggtcaccagc agctggtcga tgcggtagtt atcgatatcc
    20521 accacttcaa atttgtagcc ggcgaatttc accgaatcgg tgcgtttcgg gatcttacgc
    20581 agcataaaca tcataaagcc tccgatggtt tcgtagttgc ccgactgcgg gaactcgtca
    20641 atatccagca cgcgcatgac gtcgtcaatt ggggtgccgc cgtcaatcag ccatgagttc
    20701 tcatcacggg cgacaatctg ctcttccagc ccctgaccga ccagatcgcc catcagcgtg
    20761 gtcatcacgt cgttgagggt gatgatcccc accaccagcg cgtactcgtt catgatcacc
    20821 gcgaagtctt cacctgcggt tttaaaactt tccaacgcct ctgaaagggt taacgtatcc
    20881 ggcacaatca gcgtgttgcg aatttgtacg ccgctgttca gtgccaggct ttggttagcc
    20941 agcacgcggt tcagcaggtc tttagaatcg acataaccga tgatgtggtc aatatcttca
    21001 ttacagacga ggaacttaga gtgcggatgt tccgccacct tattcttcag gctttgctca
    21061 tcttcgtgga gatcaaacca aatcacgttt tcacgcggtg tcattgaaga cggaacggta
    21121 cgggattcca gctcaaagac gttttcaatc agctcgtgtt cctgtttacg taacacgccc
    21181 gccagcgcac cggcttccac taccgcgtag atgtcatcag aagtgatgtc atctttacgt
    21241 accattggca gtttgaaaat acggaagatc atgttcgcca ggccgttgaa gaaccacacc
    21301 agcggggtgc aaacgtacag gcagaagcgc atcgggttga tgatacgcaa agccaccgct
    21361 tctggcgcaa tcataccgat gcgtttcggg gttaaatccg caaacaggat aaacatgcca
    21421 gtcactaacg agaaagagag aataaagctc agttgctcag agagttctgc cgacatatag
    21481 cgggagaaca ggctgtgaaa agctggagaa aatgccgcat caccgacgat accgccgaga
    21541 atcgcaactg cgttcagacc gatttggacc accgtaaaga acatgccggg attttcctgc
    21601 atattcagaa cgcgttgggc atttatattg ccttcatcag ccagcagttt aagtttgatt
    21661 ttgcgtgagg cggcaagtga gatctcagac atcgagaaga acgcacttac agcgatcaag
    21721 cagagtatga ctaaaatact gtttaacata tcttatccga cctttcaggc cagatcctcg
    21781 gaaggggaag ttgattaatt tgtgtgaaat acacattgaa agccgattcg aagaaagtga
    21841 accggcattt tcagtggcta gtatagcgta aggtactgta aagccgccag agggttaaaa
    21901 ttcagacagc tgaaaaatgc aaaactgcct gatgcgctat gccgatcagg cctacgcagt
    21961 ccttgcaata tattgaattt ggacgatttt gtaggcctgg caaggcgttt acgccgcatc
    22021 cggcataaac aaaacgcact ttatcaacaa tctacattta tacccgtaac gctatgcttc
    22081 cggcggcaga cagacgccaa ttccgccaat tccacagtag ccatacgggt ttttatgcag
    22141 atattgctgg tggtcatcct cggcataata aaacggtgtg gcgttagcga tttccgtggt
    22201 gatgcgacga tcgtcatcgg cggcaagcat cgccgcctga aaacgttcca gactggcgcg
    22261 agctgcggca tcctgttctg gggtcagcgg ataaatcgct gaacgatact gcgtgccttg
    22321 gtcattgccc tgacgcattc cctgggcagg atcgtgattc tcccaaaata cctgtagcaa
    22381 ctgctcatag ctgatgacgg aaggatcgta aacaatgcgt accgcttcgg catgaccagt
    22441 atcaccggag cacacttccc gataagtcgg atttggcgta tagccgccgg tataacctgc
    22501 ggcggtgctg taaacgccgg gtaactgcca gaacagacgc tccacacccc agaaacaacc
    22561 catcgcaaaa atggcaatct ccattccgtc aggtacattg gtcattgagt gaccgttgac
    22621 cgcatgcagc gtggctacgg gcatcggggt gttacgtcca ggcagggcat cggcggggga
    22681 aacaagatgc tttttatcaa ataaactcat ggtgtcgctc tcccgaaaat cggtcattgg
    22741 ggttaaggtt gtaacaagag gcgtatttgc acacaataac caccgtgaat aggtctaaag
    22801 taaaacataa gaaatatttg gggtttagtc tgctttttaa tccatattac tggatttttg
    22861 ttaagccgtt taacggcgtt ccaggggcag gaaaaaagga tattcaggag aaaatgtgcg
    22921 ctatatccga cagttatgct gtgtaagctt actctgctta agcggatctg ccgtcgccgc
    22981 gaacgtccgt ctacaggtcg aggggttatc gggacagctg gaaaagaacg ttcgtgcgca
    23041 gctttctacg attgaaagtg atgaagtgac gccagaccgt cgctttcgcg cacgcgtcga
    23101 tgatgccatc cgcgaaggtc tgaaagcgct gggttattac cagccgacca ttgaatttga
    23161 tctccgtcca ccgccaaaga aagggcggca ggtattgatc gccaaagtta cgccaggcgt
    23221 gccggtgtta attggcggca ccgatgtggt attgcgcggc ggcgcgcgga ccgataaaga
    23281 ctatttgaaa ttgctcgata ctcgcccggc tattggcacg gtactgaacc agggcgatta
    23341 tgaaaatttc aaaaagtcct taaccagcat tgcgttgcgt aaaggttatt tcgatagcga
    23401 atttaccaaa gcgcagctgg gcattgcgct cggcctgcat aaagccttct gggatattga
    23461 ttataacagt ggcgaacgtt accgctttgg gcatgtgacc tttgaaggat cacaaatccg
    23521 cgatgaatac ctgcaaaatc tggtgccgtt taaagagggc gatgagtacg aatcgaaaga
    23581 tctggcggaa ctgaaccgcc gactttctgc taccggctgg tttaactcgg tggtggtggc
    23641 tccacaattt gataaagcgc gcgaaacgaa agtattaccc ttgacgggcg tggtttcgcc
    23701 gcgaactgaa aacaccatcg aaaccggggt cggttactct acggacgtgg gaccgcgcgt
    23761 gaaagcgacg tggaaaaagc cgtggatgaa ctcttatggt cacagtctga ccaccagtac
    23821 cagtatttcc gcgccggaac agaccctcga cttcagctat aaaatgccgc tgctgaagaa
    23881 tccactggaa caatattatt tggtgcaggg cggttttaag cgcactgacc tgaacgatac
    23941 cgaatctgac tccactacgc tggtggcttc tcgctactgg gatctctcca gcggctggca
    24001 gcgtgccatt aacctgcgct ggagcctcga ccactttacc cagggtgaaa ttaccaacac
    24061 cacgatgctg ttttatcctg gggtgatgat tagccgcacg cgttctcgtg gtggcctgat
    24121 gccaacctgg ggcgactcgc aacgctactc tatcgactac tccaacacgg cctggggctc
    24181 agatgtcgat ttctccgttt tccaggcgca gaacgtctgg atccgcacac tgtacgatcg
    24241 ccatcgtttt gtgacacgcg gcacgctggg ctggattgaa acgggtgatt tcgacaaagt
    24301 accgccggat ctgcgtttct tcgccggggg cgatcgcagt attcgcggct acaaatacaa
    24361 atctatcgcc ccgaaatacg ccaacggtga cctgaaaggg gcctcgaagc tgataaccgg
    24421 atcgctggaa taccagtaca acgtgaccgg aaaatggtgg ggcgcggtgt ttgtcgatag
    24481 tggcgaagcg gtaagcgata ttcgccgcag cgactttaaa accggtaccg gggtcggcgt
    24541 acgctgggaa tcgccggtcg ggccaatcaa actcgatttt gccgtaccgg tcgcggataa
    24601 agacgagcac gggttacagt tttacatcgg tctggggcca gaattatgag tttatggaaa
    24661 aaaatcagcc tcggcgtggt tatcgttatc ttactgttgc tgggatcggt ggcgtttctg
    24721 gtgggcacca ccagcggcct gcatcttgta tttaaagcgg cggatcgctg ggtgccagga
    24781 ctggatattg gcaaggtcac cggcggctgg cgcgatctca ccttgtctga cgttcgttat
    24841 gagcagccag gcgtggcggt aaaagcgggt aatctgcatc tggcggtcgg tcttgagtgc
    24901 ctgtggaaca gtagcgtttg tattaatgac ctggcgctga aagacattca ggtcaacatc
    24961 gacagtaaaa aaatgccccc ttctgaacag gttgaagaag aggaagatag cggtccgctg
    25021 gatctctcca cgccgtatcc catcaccctg acacgggtgg cgctggataa cgtcaacatc
    25081 aagattgatg acaccacggt atcggtgatg gacttcacct ccggcctgaa ctggcaggag
    25141 aaaaccctga ccctgaaacc gacgtcgctg aaaggcctgt tgattgccct gccgaaagtg
    25201 gcggaagtgg cgcaggaaga agtggtcgaa ccgaaaattg aaaatccgca gccggatgaa
    25261 aagccgctcg gcgaaacgtt gaaagatctc ttttctcgtc cggtattgcc ggaaatgacc
    25321 gacgtgcatt tgccgcttaa cctgaacatt gaagagttta agggcgagca gctgcgcgtg
    25381 acgggcgaca cggacatcac cgtgaacacc atgctgctga aagtaagcag catcgacggt
    25441 aatactaaac tggacgccct ggatatcgat tccaatcaag ggatcgtcaa cgccagcggc
    25501 acggcacagc tgtcagacaa ctggccggtg gatatcaccc tcaacagcac actaaacgtg
    25561 gagccgttga aaggtgaaaa agtgaagctg aaagtgggcg gcgcgctgcg cgaacagctg
    25621 gagattggcg ttaacctttc cggtccggtg gatatggatt tacgcgccca gacgcgactg
    25681 gcggaagctg gattgccgct caacgtggaa gtgaacagca aacagcttta ctggccgttc
    25741 actggtgaga agcagtatca ggcggatgat ctgaaactga aacttaccgg caaaatgacc
    25801 gattacacgc tctctatgcg tacggcagtg aagggacagg agatcccgcc cgcgactatt
    25861 accctcgacg cgaaaggtaa tgaacagcag gtcaatctcg acaaactcac cgttgcggcg
    25921 ctggaaggca aaactgaact caaggcgttg ctcgactggc aacaggccat tagttggcgc
    25981 ggtgagctaa cgcttaatgg cattaacacc gccaaagagt tcccggagtg gccgtcgaaa
    26041 ctcaatggct tgattaaaac ccgcggtagc ctgtacggcg gcacctggca gatggaggtg
    26101 ccagaactga agctgaccgg taacgtcaaa cagaacaaag tgaacgttga cggcacgctg
    26161 aaaggcaaca gttatatgca gtggatgatc ccagggcttc atctggaact ggggccaaac
    26221 agtgccgaag tgaaaggcga gctgggggta aaagatctca atcttgatgc caccatcaac
    26281 gcgccggggc tggataacgc gctgccgggg cttggcggta cagcgaaagg gctggtgaaa
    26341 gtacgcggca cggtggaagc gccacaacta ctggcagata tcaccgcgcg cggcctgcgc
    26401 tggcaggaac tttccgtggc gcaggttcgc gtggaaggcg acattaaatc caccgatcag
    26461 atcgccggga aactcgacgt acgcgttgag caaatttcgc agccggatgt aaatatcaac
    26521 ctcgtcaccc tgaatgccaa aggcagcgaa aagcagcacg agctacagtt gcggattcag
    26581 ggcgagcctg tctccgggca gcttaatctg gcaggaagtt ttgatcgcaa agaagaacgc
    26641 tggaagggaa ctcttagcaa tacccgcttc cagacgccgg tcggcccgtg gtcgctgacc
    26701 cgcgatattg cgctggatta ccgcaataag gagcaaaaaa tcagcatcgg gccacactgt
    26761 tggcttaacc cgaatgcgga gctgtgcgtg ccgcaaacta tcgatgcggg ggccgaaggg
    26821 cgtgcggtgg tgaatctcaa ccgcttcgac ctcgccatgc tgaaaccgtt tatgccagaa
    26881 accactcagg ccagcggtat cttcacgggt aaagcggatg ttgcctggga caccacgaaa
    26941 gaggggctgc cgcagggcag tatcaccctt tcggggcgta acgtgcaggt aacgcaaacc
    27001 gtcaacgatg cggcgctgcc cgtggcgttt cagacgctga atctgacggc ggaattgcgt
    27061 aacaaccgtg ccgaattggg ctggaccatc cgcctgacca ataacggcca gtttgatgga
    27121 caggtgcagg tgaccgatcc gcaaggccgc cgtaatcttg gtggcaacgt caatatccgt
    27181 aacttcaacc ttgcgatgat aaaccccatc tttacccgtg gggaaaaagc agcggggatg
    27241 gtgagtgcca acttgcgtct gggtggtgat gtgcaaagcc cgcagttgtt tggtcagctt
    27301 caggttacgg gtgtggatat cgacggcaac tttatgccgt ttgatatgca accgagccag
    27361 cttgcggtca actttaacgg tatgcgctcg acgcttgccg gtacagtacg gacccagcag
    27421 ggtgaaatct acctgaacgg tgatgccgac tggagccaaa ttgaaaactg gcgggcgcgg
    27481 gtaacggcga aaggcagtaa agtgcggatc accgtgccgc cgatggtacg aatggatgta
    27541 tcgccagatg ttgtattcga ggctacacca aacctgttta ccctcgatgg tcgcgtggat
    27601 gtcccttggg cgcgcatcgt ggtgcacgat ctgccggaaa gcgcagtagg cgtctccagc
    27661 gatgtggtga tgcttaacga taacctgcaa ccggaagagc cgaaaacggc gtcgattccg
    27721 attaacagta acctgattgt ccacgttggc aataatgtgc gcattgacgc ctttggcctg
    27781 aaagcgcggc tgacgggcga tcttaacgtc gttcaggaca aacaagggct gggcctgaac
    27841 gggcagatca acatccctga agggcgcttc catgcctatg gtcaggatct gattgtgcgt
    27901 aagggtgagt tactgttctc tggtccgccg gatcaaccgt atcttaatat tgaagctatt
    27961 cgtaacccgg atgctacaga agacgacgta atcgccggag ttcgcgtcac tggtctggcg
    28021 gacgaaccga aagcggagat cttctctgac ccggcgatgt cgcaacaagc tgcattgtct
    28081 tatttgctac gtggacaagg gctggagagc gatcagagcg acagtgcggc aatgacctcg
    28141 atgctgattg gtctgggggt tgcgcaaagt ggccagattg tgagtaaaat cggcgagacg
    28201 tttggcgtaa gcaatttagc gctcgacacc cagggagtag gcgactcctc ccaggtagtg
    28261 gtcagcggct atgtattgcc aggtctgcaa gtgaaatatg gcgtgggtat atttgactct
    28321 atagcaacac tcacgttacg ttatcgcctg atgcctaagc tatatctgga agccgtgtct
    28381 ggtgtagacc aggcactgga tttgctctat cagttcgagt tttagcaatg cgaatatttg
    28441 tctacggcag tttacgccac aaacaaggca acagtcactg gatgaccaat gcccagttac
    28501 tgggcgattt cagtatcgat aactaccagt tgtatagcct gggccactat ccaggcgcag
    28561 ttccggggaa cggaacagta cacggtgaag tttatcgtat tgacaacgcc acgctggccg
    28621 aacttgatgc cttgcgcacc aggggcggtg aatacgcgcg ccagttgatt cagacgccgt
    28681 acgggagtgc atggatgtac gtttatcaac gacccgtcga tggattaaag ctaattgaaa
    28741 gcggcgactg gttagacagg gataagtaac catatgcata cgccaccttc gg
//
LOCUS       DAZFQD010000057        28425 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.057, whole genome shotgun sequence.
ACCESSION   DAZFQD010000057 DAZFQD010000000
VERSION     DAZFQD010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28425
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.057"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(144..398)
                     /gene="ymgE"
                     /locus_tag="ACHKTW_002509"
     CDS             complement(144..398)
                     /gene="ymgE"
                     /locus_tag="ACHKTW_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFO6463514.1"
                     /translation="MGIIAWIIFGLIAGIIAKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIGGSISGFNLHSFLVAVVGAILVLGIFRLLRRE"
     gene            599..1333
                     /gene="ycgR"
                     /locus_tag="ACHKTW_002510"
     CDS             599..1333
                     /gene="ycgR"
                     /locus_tag="ACHKTW_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HFO6463515.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRLSWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AELQEGMRFAQIEVNMRQWGVFHFDAQLISISERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            complement(1335..1946)
                     /gene="emtA"
                     /locus_tag="ACHKTW_002511"
     CDS             complement(1335..1946)
                     /gene="emtA"
                     /locus_tag="ACHKTW_002511"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HFO6463516.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            2046..2960
                     /gene="ldcA"
                     /locus_tag="ACHKTW_002512"
     CDS             2046..2960
                     /gene="ldcA"
                     /locus_tag="ACHKTW_002512"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HFO6463517.1"
                     /translation="MSLFHLIAPSGYCIKQHAALRGIQRLTDEGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQYDPLLI
                     CGHSDFTAIQCGLLAQGNVITFSGPMLVANFGADELNAFTEHHFWLALRNETFTIEWQ
                     GEGPTCQTEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINEHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTREGTQLTISGHPVLKM"
     gene            3055..4791
                     /gene="cvrA"
                     /locus_tag="ACHKTW_002513"
     CDS             3055..4791
                     /gene="cvrA"
                     /locus_tag="ACHKTW_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006884 - cell volume homeostasis [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HFO6463518.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(5177..6247)
                     /gene="dadX"
                     /locus_tag="ACHKTW_002514"
     CDS             complement(5177..6247)
                     /gene="dadX"
                     /locus_tag="ACHKTW_002514"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309712.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HFO6463519.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGVTDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISSAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGTPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALALRVPVVTV"
     gene            complement(6257..7555)
                     /gene="dadA"
                     /locus_tag="ACHKTW_002515"
     CDS             complement(6257..7555)
                     /gene="dadA"
                     /locus_tag="ACHKTW_002515"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HFO6463520.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVEPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKCGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQSTFWTGLRPMTPDGTPVVGRTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            7885..9417
                     /gene="ycgB"
                     /locus_tag="ACHKTW_002516"
     CDS             7885..9417
                     /gene="ycgB"
                     /locus_tag="ACHKTW_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HFO6463521.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNYLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGWATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSIELLERCPPRMG
                     NL"
     gene            complement(9469..10188)
                     /gene="fadR"
                     /locus_tag="ACHKTW_002517"
     CDS             complement(9469..10188)
                     /gene="fadR"
                     /locus_tag="ACHKTW_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000062 - fatty-acyl-CoA binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0019217 - regulation of fatty acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HFO6463522.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            10409..11950
                     /gene="nhaB"
                     /locus_tag="ACHKTW_002518"
     CDS             10409..11950
                     /gene="nhaB"
                     /locus_tag="ACHKTW_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HFO6463523.1"
                     /translation="MEISWGRALWRNFLYQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHIREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYKVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            12096..12626
                     /gene="dsbB"
                     /locus_tag="ACHKTW_002519"
     CDS             12096..12626
                     /gene="dsbB"
                     /locus_tag="ACHKTW_002519"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707082.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HFO6463524.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGMEMPQWLLGIFIAYLIVAVLV
                     VISQPFKAKKRDLFGR"
     gene            complement(12672..13940)
                     /gene="umuC"
                     /locus_tag="ACHKTW_002520"
     CDS             complement(12672..13940)
                     /gene="umuC"
                     /locus_tag="ACHKTW_002520"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HFO6463525.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVRNCRDLTDFGREIRATVLQRTHLTVGVGIAQTKTLAKLANHAAKKWQRQT
                     GGVVDLSNLERQRKLMSALPVDDVWGIGRRISKKLDAMGIKTVLDLADTDIRFIRKHF
                     NVVLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYPSMRQAICSYAARAAE
                     KLRSEHQYCRFISTFIKTSPFALNEPYYGNSASVKLLTPTQDSRDIINAATRSLDAIW
                     QAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMTVMDTLNAKEGRGTLYFA
                     GQGIQQQWQMKRAMLSPRYTTRSSDLLRVK"
     gene            complement(13940..14359)
                     /gene="umuD"
                     /locus_tag="ACHKTW_002521"
     CDS             complement(13940..14359)
                     /gene="umuD"
                     /locus_tag="ACHKTW_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HFO6463526.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
     gene            14732..15642
                     /gene="hlyE"
                     /locus_tag="ACHKTW_002522"
                     /pseudo
     CDS             14732..15642
                     /gene="hlyE"
                     /locus_tag="ACHKTW_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin HlyE"
     gene            complement(15849..16295)
                     /gene="ycgN"
                     /locus_tag="ACHKTW_002523"
     CDS             complement(15849..16295)
                     /gene="ycgN"
                     /locus_tag="ACHKTW_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HFO6463527.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLAEGKDL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(16387..17046)
                     /gene="ycgM"
                     /locus_tag="ACHKTW_002524"
     CDS             complement(16387..17046)
                     /gene="ycgM"
                     /locus_tag="ACHKTW_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HFO6463528.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEQRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLATRV
                     L"
     gene            complement(17118..17411)
                     /locus_tag="ACHKTW_002525"
     CDS             complement(17118..17411)
                     /locus_tag="ACHKTW_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HFO6463529.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKLALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            17653..18054
                     /gene="pliG"
                     /locus_tag="ACHKTW_002526"
     CDS             17653..18054
                     /gene="pliG"
                     /locus_tag="ACHKTW_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="g-type lysoyme inhibitor PliG"
                     /protein_id="HFO6463530.1"
                     /translation="MKIKSISKAVLLLALLTSTSFAAGKNVNVEFSKGHSSAQYSGEI
                     KGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPGIDDSVDLSRYSPELDSHGQYSLP
                     ASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
     gene            complement(18174..18542)
                     /gene="ycgJ"
                     /locus_tag="ACHKTW_002527"
     CDS             complement(18174..18542)
                     /gene="ycgJ"
                     /locus_tag="ACHKTW_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgJ family protein"
                     /protein_id="HFO6463531.1"
                     /translation="MNMMRIFYIGLSGVGMMFSSMASGHDAGGLQSPACGVVCDPYIC
                     VNSDGISPELTRKYLGEKAAENLQSLQGYDPSEFTFANGVFCDVKEKLCRDDRYFGVD
                     GKRSGKINQITTKMLFMCRE"
     gene            19062..19757
                     /gene="minC"
                     /locus_tag="ACHKTW_002528"
     CDS             19062..19757
                     /gene="minC"
                     /locus_tag="ACHKTW_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA];
                     GO_process: GO:1901891 - regulation of cell septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HFO6463532.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALEDKIAQAPAFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTTPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            19781..20593
                     /gene="minD"
                     /locus_tag="ACHKTW_002529"
     CDS             19781..20593
                     /gene="minD"
                     /locus_tag="ACHKTW_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HFO6463533.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            20597..20863
                     /gene="minE"
                     /locus_tag="ACHKTW_002530"
     CDS             20597..20863
                     /gene="minE"
                     /locus_tag="ACHKTW_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032955 - regulation of division septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HFO6463534.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(21235..21579)
                     /locus_tag="ACHKTW_002531"
                     /pseudo
     CDS             complement(21235..21579)
                     /locus_tag="ACHKTW_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(21614..21733)
                     /locus_tag="ACHKTW_002532"
     CDS             complement(21614..21733)
                     /locus_tag="ACHKTW_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HFO6463535.1"
                     /translation="MPLSYAGYHHFIMMSLVSGEHNTPALSLQDESPGLVPAG"
     gene            complement(21694..21879)
                     /gene="ymgJ"
                     /locus_tag="ACHKTW_002533"
     CDS             complement(21694..21879)
                     /gene="ymgJ"
                     /locus_tag="ACHKTW_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgJ"
                     /protein_id="HFO6463536.1"
                     /translation="MSRRYSSATITLVILFCRRPHCRKISVRQYYLKRVIFYPTTLQR
                     TACKKCLFLMRDIIISS"
     gene            21980..22153
                     /gene="ymgI"
                     /locus_tag="ACHKTW_002534"
     CDS             21980..22153
                     /gene="ymgI"
                     /locus_tag="ACHKTW_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgI"
                     /protein_id="HFO6463537.1"
                     /translation="MSYSSFKIILIHVKNIIPIITATLILNYLNNSERSLVKQILIED
                     EIIVCATYLIPDI"
     gene            22155..22499
                     /gene="ymgG"
                     /locus_tag="ACHKTW_002535"
     CDS             22155..22499
                     /gene="ymgG"
                     /locus_tag="ACHKTW_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YMGG-like glycine zipper-containing protein"
                     /protein_id="HFO6463538.1"
                     /translation="MKKKILAFGLISALFCSTPAMADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVGAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            22503..22838
                     /gene="ymgD"
                     /locus_tag="ACHKTW_002536"
     CDS             22503..22838
                     /gene="ymgD"
                     /locus_tag="ACHKTW_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HFO6463539.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(22895..25216)
                     /locus_tag="ACHKTW_002537"
     CDS             complement(22895..25216)
                     /locus_tag="ACHKTW_002537"
                     /inference="COORDINATES: protein motif:HMM:TIGR01414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFO6463540.1"
                     /translation="MQGKNTIVITGDYSIGLLSQTSGNLNTDTIIRVNSDGSVTPSFS
                     DGDDTFIVTAGNHAVGVLACASPGSARACVSSLDEESTTDTGSNENNAIAKLDMAKGE
                     ITTHGTESYAAYANGTVVKAVDTLDYTNASVTLTDVDITTHGDNAHAIAARQGTVSFN
                     QGEIYTTGPDAATAKIYNGGTVTLKNTSAVAHQGSGIVLESSINGQEATVDILSGSSL
                     RSANEILYHKNETSNVTITDSEVSSAADVFINNIKGHLTVDATNSKITGSANISTDVN
                     THTYLSLSDNSTWDIKADSTVSNLTVDNSTVYISRADGRDVEPTRLTITENYVGNNGV
                     LHLRTELGDDNSATDKVVINGNTSGTTRVKVTNAGGSGAYTLNGIEIISVEGESNGEF
                     IKDSRIFAGAYEYSLTRGNTEATNKNWYLTNFQATSGGETNSGGSSAPTVAPTPVLRL
                     EAGSYVANLAAANTLFVMRLNDRAGEMRYIDPVTEQERSSRLWLRQIGGHNAWRDSNG
                     QLRTTSHRYVSQLGADLLTGGFTDSDSWRLGVMAGYARDYNSTHSSVSDYRSKGSVRG
                     YCAGLYATWFADDISKKGAYIDAWAQYSWFKNSVKGDELAYESYSAKGATVSLEAGYG
                     FALNKSFGLEAAKYTWIFQPQAQAIWMGVDHNAHTEANGSRIENDANNNIQTRLGFRT
                     FIRTQEKNSGPHGDDFEPFVEMNWIHNSKDFAVSMNGVKVEQDGASNLGEIKLGVNGN
                     LNPAASVWGNVGVQLGDNGYNDTAVMVGLKYKF"
     gene            complement(25333..25542)
                     /locus_tag="ACHKTW_002538"
     CDS             complement(25333..25542)
                     /locus_tag="ACHKTW_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6463541.1"
                     /translation="MKLKKLPGFSLGLIALAVGNAYAEQFIQHDEYKHAVAIKDGKVK
                     DIDNALVITTGNGSYGYPLQERVAS"
     gene            complement(25942..26160)
                     /gene="ymgF"
                     /locus_tag="ACHKTW_002539"
     CDS             complement(25942..26160)
                     /gene="ymgF"
                     /locus_tag="ACHKTW_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division-associated protein YmgF"
                     /protein_id="HFO6463542.1"
                     /translation="MNNSNNLDYFTLYIIFSIAFMLITLLVILIAKPSTGLGEVLVTI
                     NLLNALVWLAINLVNRLRERLVSHRDQQ"
     gene            complement(26292..27815)
                     /gene="pdeG"
                     /locus_tag="ACHKTW_002540"
     CDS             complement(26292..27815)
                     /gene="pdeG"
                     /locus_tag="ACHKTW_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFO6463543.1"
                     /translation="MRNTLIPILVAICLFITGVAILNIQLWYSAKAEYLAGARYAANN
                     INHILEEASQATQTAVNIAGKECNLEEQYQLGTEAALKPHLRTIIILKQGIVWCTSLP
                     GNRVLLSRIPVFPDSNLLLAPAIDTVNRLPILLYQNQFADTRILVTISDQHIRGALNV
                     PLKGVRYVLRVADDIIGPTGDVMTLNGHYPYTEKVHSTKYHFTIIFNPPPLFSFYRLI
                     DKGFGILIFILLIACAAAFLLDRYFNKSATPEEILRRAINNGEIVPFYQPVVNGREGT
                     LRGVEVLARWKQPHGGYISPAAFIPLAEKSGLIVPLTQSLMNQVARQMNAIASKLPEG
                     FHIGINFSASHIISPTFVDECLNFRDSFTRRDLNLVLEVTEREPLNVDESLVQRLNIL
                     HENGFVIALDDFGTGYSGLSYLHDLHIDYIKIDHSFVGRVNADPESTRILDCVLDLAR
                     KLSISIVAEGVETKEQLDYLNQNNITFQQGYYFYKPVTYIDLVKIILSKPKVKVVVE"
     gene            complement(28147..28395)
                     /gene="ymgC"
                     /locus_tag="ACHKTW_002541"
     CDS             complement(28147..28395)
                     /gene="ymgC"
                     /locus_tag="ACHKTW_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgC"
                     /protein_id="HFO6463544.1"
                     /translation="MNNSIPERFIFQCALFKNLEREVFMTHGYVDSHIIDQALRLRLK
                     DETSVILSDLYLQILQYIEMHKTTLTDIIINDRESVLS"
BASE COUNT         7384 a         7030 c         6619 g         7392 t
ORIGIN      
        1 tctgttgttg ttaccgggaa ccgactttat cggatatcga agtgctatat tgattttttt
       61 cacccgtaaa gcaggtgtcg agtatacgtt ctgatgtgaa agcggatagc ggcatctgta
      121 tcgctatccg ccttatgaaa atcttattct cttcgcagga ggcggaatat gcccagaacg
      181 agaatagctc ccaccaccgc caccaggaag ctgtgcagat taaaaccact gatggagccg
      241 ccaatgccaa acatggtcgc cagccagccg ccgaccaccg caccgactat cccgagaata
      301 caggtcagga aaaatccacc accatcacgc cccggcatga ttagcttggc gataatgccg
      361 gctatcaggc caaaaataat ccaggcaata attcccatgt tcaggccctc gtttcgatga
      421 atcggacgca agaaatgctt acgtcaacgc tctaagtata ggcaagggcg ggatcgatgc
      481 tgggcgtatg tcggctgatt taagaacaac atgtaaaaaa tattcacgca tttgtattaa
      541 gttttgttaa ctgtgaccga taaaccaaag acagtttatc agtcaggagt ttttccgcgt
      601 gagtcattac catgagcagt tcctgaaaca aaatccgtta gccgtcctgg gcgtgttacg
      661 cgatttgcac aaagccgcaa ttcctttgcg tctcagttgg aatggcgggc agctgatcag
      721 caaaatactg gcaataaccc cggataaact ggtgctggat ttcggcagtc aagccgaaga
      781 caacatcgcc gtgctaaagg cacagcacat taccattacc gccgaaactc agggtgcgaa
      841 agtcgagttt actgttgaac aactacagca gagtgaatac ttgcagcttc cggcatttat
      901 caccgtaccg cctcccacct tatggtttgt acaacgacgc cgatatttcc gcatctccgc
      961 cccactccat ccgccttatt tttgccagac caaactggcg gataacagta cgttacgttt
     1021 ccgcctgtat gatttgtcgt taggcggcat gggcgcatta ctggaaacag caaagcctgc
     1081 cgaattacaa gaaggcatgc gcttcgctca gattgaagtc aacatgaggc aatggggtgt
     1141 ttttcacttt gacgcccagt taatctccat cagcgagcgc aaagtgattg atggcaagaa
     1201 tgaaaccatc accactcccc gtctgagctt ccgttttctt aacgtcagcc cgacggtgga
     1261 gcggcaatta cagcggatta ttttctctct cgagcgagaa gcccgggaaa aagcggacaa
     1321 agtgcgcgac tgatttacat cgcgtccagt gcctgctcaa gtttgtagat atagcgcgga
     1381 gcctgcggcg cagggtgatt tcgcgctacg tgatcgagga actcgtcagc atccaaatcg
     1441 ttgattttgc tgatcgcctt tttccgatct gacgagaaag tccgtagcag cgcacctgcc
     1501 ccgttagcgt atgacaccac cagcgcatat tgcagtacct tcggatcttc aatgcctgcc
     1561 agcgggccgg tttccagaat attcaggtaa gccgccccca ttgaaatatt acgctctgga
     1621 tttttcagct cgctggtcgt cggctcacca ctccagccca tacggcgata aacatcacgt
     1681 ccggaggttg aagcttttaa ctgcatcaaa ccaatggcat tcgatttact caccgcattc
     1741 ggattaccac ccgattcgat agcgataatc gccgtgatca attgtggatc gacgccccag
     1801 gctgcaccgg ctttctggct tattggcatc cactgcatcg cacgttgcac cggaactttc
     1861 gcgttccacg gcgggttcgt atagtcatgc tttgatgaac aacccgctaa taacacaatc
     1921 aaaaaggcaa accatctcaa tttcacttct tctatcctta tagcctggaa tcgccgttga
     1981 ggtgacaacg gcacacctaa gcggcatgat atacatttcg tttctgttgt cagcaaggaa
     2041 ttgccatgtc tctgtttcac ttaattgccc catcgggtta ctgcattaaa cagcacgccg
     2101 cgctgcgtgg tatccagcgc cttaccgacg aggggcatca ggtcaataac gtcgaggtta
     2161 ttgcccgtcg ttgtgagcgt tttgcgggta cggaaacgga gcgcctggaa gatctcaatt
     2221 cccttgccag actgaccacc cccaacacca tcgtgctggc tgtacgcggc ggttacggtg
     2281 ccagtcgttt actggcagat attgactggc aggcactggt tgctcgccaa caatatgacc
     2341 cgttgctcat atgcgggcat agcgatttta ccgccattca gtgcggtctt ctggcgcaag
     2401 gcaatgtcat cacctttagc ggccccatgc tggtagcaaa ctttggcgcg gatgaactga
     2461 acgcctttac cgagcaccat ttctggctgg cgttacgcaa tgaaaccttc accattgaat
     2521 ggcaagggga aggcccgaca tgtcagaccg aaggcacgtt gtggggaggc aatcttgcga
     2581 tgctgatttc actgattggt acaccgtgga tgccaaaaat tgagaacggt attctggtgc
     2641 tggaagatat taacgagcac cctttccgcg tcgaacgtat gctgttgcag ctctatcatg
     2701 cgggaatttt gccacgtcag aaggcgatta ttctcggtag ctttagcggc agcacgccca
     2761 atgattatga cgcaggttac aacctggagt cagtgtacgc gtttttgcgt tcccgcctgt
     2821 cgattccact cattaccggc ctcgattttg gtcatgaaca acgcacggtt acgctgccgt
     2881 tgggcgcaca tgccattctg aataacaccc gggaaggcac tcagttgacg attagcggtc
     2941 atcctgttct taaaatgtaa aaaaatcacg cttcagggct aagtaaaacg ctgtctctgc
     3001 cgctaatatg gtagggttta tataagagac agcgtaatca ggagtaaccg accgttggat
     3061 gccacaacaa taattagcct tttcatctta ggatccatcc ttgtcaccag cagtatatta
     3121 cttagttcat tttcttcccg tcttggcatt cctattctgg ttatttttct ggcgatcggc
     3181 atgctggcag gagtcgatgg cgtcggcggt atcccgtttg ataattaccc cttcgcctac
     3241 atggtaagta acctggcact ggcgattatt ttgctcgacg gcgggatgcg cactcaggcc
     3301 agttcctttc gtgtggcgtt aggaccggca ctgtcgctgg cgacgctggg cgtgcttatc
     3361 acctctggtt taaccggcat gatggcggcg tggctgttta atcttgattt gattgaaggc
     3421 ttattaatcg gcgctatcgt cggctctacc gatgctgcag cggtcttttc tctgctgggt
     3481 ggtaaggggc ttaacgaacg tgttggctcg acgctggaaa ttgaatccgg cagtaatgat
     3541 ccaatggcgg tctttctgac gattaccctg attgcgatga tccagcaaca tgaaagcagt
     3601 gtcagttgga tgttcgtggt cgatattctg cagcaatttg gcctcgggat tgtcatcgga
     3661 cttggcggtg gttatttact gctgcaaatg attaatcgca tcgccctgcc tgccggatta
     3721 tatccattgc tggcattaag cggcggtatt ttaatttttg ctttaaccac ggcgctggaa
     3781 ggcagcggta ttctggctgt ttatctgtgc ggttttctgc tgggtaatcg gccgattcgc
     3841 aaccgctacg gcatcctgca aaatttcgac ggcctcgcct ggctggcgca aatcgccatg
     3901 ttcctggtgc tggggctatt ggttaaccca agcgatctgc tgcctattgc cattccggcg
     3961 ctcattttgt cggcatggat gatattcttc gcccgtcccc tttcggtatt tgctggattg
     4021 ctccccttcc gcggtttcaa tctgcgtgag cgcgtgttta tcagctgggt aggattacgc
     4081 ggcgcggtgc caatcatcct ggccgtgttc ccgatgatgg cggggctgga gaatgcacgt
     4141 ctgttcttta atgtcgcctt ctttgtggtt ctggtttcac tgctcttgca gggaacatca
     4201 ctctcgtggg cggctaaaaa agccaaagtg gtggttccgc cagtgggacg tccggtgtca
     4261 cgcgttggcc tggatattca tccggaaaat ccgtgggagc agtttgttta tcaattgagt
     4321 gccgataaat ggtgcgtggg cgcggcactg cgcgatttgc atatgccaaa agagacgcgt
     4381 attgcggcac tgtttcgtga taaccagttg cttcatccta ccggcagcac ccgactgcgc
     4441 gaaggcgatg tgttgtgtgt aattggtcgg gaacgcgatc ttccggcgct cggtaaactg
     4501 ttcagccagt cgccgccggt cgcgctggat caacgcttct ttggtgactt cattctcgaa
     4561 gccagcgcca aatatgctga tgttgcgctg atatatggcc tggaagacgg gcgagaatat
     4621 cgtgataagc agcaaacgct gggtgaaatt gtccagcagt tgttaggcgc ggcaccggtt
     4681 gtcggtgacc aggtagagtt tgccgggatg atctggacgg tggccgagaa agaagataat
     4741 gaagtgttga agattggcgt acgggtggct gaagaggaag ctgaatctta aatttaatga
     4801 agcatctatc gccggttgcg atgctttgct gaacgcagcc tgagcctgca attgtttgtc
     4861 gtccataatt taaccttcat ttgatgttga attgaacata tcaaaatcag gcaattacac
     4921 aaatctatgt acaggctcca tcaagggtaa agcgtgattt atctgaagtc gagttcgagt
     4981 caacaatgaa gcgtaagcgt tttatgtaga acgcctgtgc ggtgagttca ggttccgggg
     5041 aaatagtgtc cattctttta atggacacta tcatgggcaa tggctctgtc acattaacaa
     5101 ctacagttgc tgaccagccg gatggatgat tttttcgcaa ccagaagacg ctgtctccga
     5161 tccggcttac aacaagttac actgtcacaa ccgggacgcg taacgccagc gcgcacatca
     5221 actcatagcc caccgttccg gcagcggcgg cgacatcatc aattttgatc tccttgcccc
     5281 acagctcaac cggcgtacca atccccgcct gcgggcaagg cgttaaatcg accgctagca
     5341 tatccatcga gacggtcccc accgtcatgg tgcgcacgcc gtccactaaa acaggggtac
     5401 cggtaggcgc gtggcgcgga taaccgtcgg cgtaccctgc ggcgacaatg ccgattcgct
     5461 gttcatcgcg cgcagtatag cgaccgccgt agcccacacg ctcacccgct tttagcgtct
     5521 ggacaccaat aatctcactg cttagcgtca tcaccggacg taatccggta ttggcgatat
     5581 cacgccactg accggacggc gaagcgccat acaaaataat gccaggccga acccagtcaa
     5641 aatgcgcttc cgggtgccac agagtcgccg ccgaattgga caacgaacgc cgacactcca
     5701 gcccttccgc cgcctgctca atacgcgcca tcgcgctgga aattccatca ggatgttccg
     5761 cttcggcaaa atgcgacatc agggtcatct cgccaacatt cgccattgcc cgcaactgct
     5821 gccagacggt aagcacgcga tcgggctgga agcccaaccg attcatccca ctgttcactt
     5881 taagataaat atccaacggt gcttttagcc gcgcattttg cagtgctttg agctgccagt
     5941 tgctgtgtac gcaggtggtc aggcggtgct ggtcataaat ctccagatcc tgagcatgga
     6001 aaaatccttc cagcatcagg atcggcccct tccagccgcg ctcacgtaac gttattgcct
     6061 cttccaggtt aagtaatgca aagccatcgg tgaccccgag cgcgctccag atacgctcaa
     6121 taccatgccc gtaagcgttc gcttttacca ccgaccaaac gcgcgcgtgc ggcgcggcct
     6181 ggcggacaat ggacagattc tgttttaatg cctgcagatc gaggctggcc tgtatcggac
     6241 gggtcatctc gtttccttag ctgtgtgcgc catgtaaatg gcccggacgt gatggcgtaa
     6301 atccacggct gtagcgcgct acgcttagat cctcgtatgg gatcgctggc gtgcgaccag
     6361 agagcagatc gcttaacaac tgaccggaac cgcaagccat cgtccagccg agcgtgccgt
     6421 gaccggtatt cagccacaga tttttaaagc gtgtacgccc gacaaccggc gtaccgtctg
     6481 gcgtcatcgg gcgcaaaccc gtccagaaag tcgactgctc gacatgaccg ccgcgtggat
     6541 agagatcgcg aaccaccatt tccagcgttt cacgacgcgg ttgcaacagc tcggtgttaa
     6601 aaccaacaat ttccgccatt ccgccaacac gaatgcggtt atcgaaacgg gtaatggcga
     6661 ttttgtaggt ttcatcaaga atggtggata ctggcgcacc atcttcctgc gcaattggaa
     6721 tagtcagcga gtagcctttc agcggataca ccggaatatc aacaatgcct ttgagcatcg
     6781 ccgtcgagta agaaccaaac gccatcacat acgcatcggc cttaatcact tcatcgccac
     6841 acttcacgcc gtagatttgc tcgccgtcgc aaagcagttg gtcaacgggc gtattaaagc
     6901 ggaatttaac ccccgcctgc tctgccatcc gcgccagatt ctgggtaaac agctggcagt
     6961 ctccggtttc atcattgggc agttgcagac cacccgtcag tttgtgcgct acttctgcca
     7021 gcgcgggctc cacttccgcc aggcggctgg attccagcag ctgatacggt acgccggcat
     7081 cttccagcac ggcgatatcg cgggtcgcat tttcatactg ttgttcggta cggaacagtt
     7141 gcagcgtccc accctgacgc ccttcatact gaatattggt ttcggcgcgc aatgctttca
     7201 ggcaatcacg gctgtattcc gccagacgca ccatccgccc tttgttttcc atgtagtggc
     7261 tggtgtcgca gttacgtaac atttgccaca tccatttcaa ctggaactgc gtaccgtcga
     7321 gacgaaccgc cagcggcgca tggcgctgga acatccattt aatcgctttt aaaggcacac
     7381 ctggtgccgc ccacggtgca gcatatccgg gggagatttg cccggcattc gcggcgctgg
     7441 tttccagggc ggcccccggc tcccgatcaa tgacggtgac ctcatgtcct gcctgattta
     7501 agtaccaggc gctggcaacg cctaccacac cacttcccag tatgacaact cgcatagcca
     7561 ctccgataac ggtagataca gtaaaagaat aataatctaa ttacatcttg ataactcagt
     7621 tgaaaatatt attcaacata cagctttttt atggtgagct ggctcacatc tccctgactc
     7681 tgcggcaata tggcgtcttt agtatctcct gctgaacgcc ctttttatcc agcataaaat
     7741 tccgttcaga agcggattag tggcactctg ataataagaa atcgcaaaga aaaaaattct
     7801 ccggcgacgc ggtttttaac aggtgttcta tgcttgaaat gaggtgctcc acacagagag
     7861 cgccgacaac aatgaggtgc gcgtatggcg acgatcgatt ctatgaataa ggacaccaca
     7921 cgtttgagcg atggacccga ctggacgttc gacctgctgg atgtttatct ggcagagata
     7981 gaccgggtgg cgaaactcta ccggctggat acctacccgc accagattga agtgataacc
     8041 tcagaacaga tgatggatgc ctactccagc gtcggcatgc caattaacta tccgcactgg
     8101 tcattcggta aaaagtttat cgagactgaa cggctgtata agcacggtca gcaaggactg
     8161 gcctatgaaa tcgtcattaa ctctaacccg tgtatcgctt acctgatgga agagaacacc
     8221 attaccatgc aggcgctggt gatggcacac gcctgctatg ggcataactc tttctttaaa
     8281 aacaattact tattccgcag ttggaccgac gccagttcga ttgtcgatta tctgattttt
     8341 gcccgtaaat atattaccga gtgcgaagaa cgttatggcg ttgatgaagt agaacggctt
     8401 ctggactcgt gccacgcgct gatgaactac ggcgtggacc ggtacaaacg cccgcaaaaa
     8461 atctcgctgc aagaagagaa agcccggcag aaaagtcgcg aagagtatct acaaagtcag
     8521 gtcaatatgc tctggcgtac cctgccgaag cgcgaggaag agaaaacggt tgctgaagcg
     8581 cgccgctatc cgtccgaacc acaagaaaac ctgctctatt ttatggagaa aaatgcgcca
     8641 ctgctggaat catggcagcg tgaaatcctg cgtattgtgc gtaaggtgag ccagtatttt
     8701 tatccgcaaa aacagactca ggtgatgaac gaaggctggg cgaccttctg gcactatacc
     8761 atccttaacc atctgtatga tgaagggaaa gtaacggaac gttttatgct ggagtttttg
     8821 cacagccaca ccaatgtggt cttccagccc ccctataaca gcccgtggta cagcggcatc
     8881 aacccgtatg ccctcgggtt cgccatgttc caggatatta aacggatttg tcagtcgcca
     8941 acggaagaag acaaatactg gttcccggat atcgccggtt ccgactggct ggaaacgctg
     9001 catttcgcga tgcgtgattt caaagatgag agttttatca gccagtttct gtcaccgaaa
     9061 gtgatgcgtg atttccgctt cttcaccgtg ctggatgatg atcggcataa ttatctggag
     9121 atttccgcta ttcataatga agaaggttat cgggagatcc gtaaccggtt atcgtcgcaa
     9181 tataacttaa gtaatctgga gccgaatatt cagatctgga acgtggattt gcgcggcgac
     9241 cgttcgctga cgctgcgtta cattccacat aatcgcgcac cgctggatcg ggggcgcaaa
     9301 gaagtgctga agcatgtgca tcgcctgtgg ggatttgatg tgatgctcga acagcaaaac
     9361 gaagacggca gcatcgagtt gctggaacgt tgcccgccaa gaatgggaaa tctgtaaaaa
     9421 caacaaaaaa cccctcgttt gaggggtttg ctctttaaac ggaagggatt atcgcccctg
     9481 aatggctaaa tcacccggca gatttttctg catccggtgc caaatctcgc cactctcatg
     9541 cccatagcga cgcactgttt cgtacacctg atcgtgcgcg ccttcactgc acaacgccga
     9601 cagtttgtgg tagaagccca gcgccagact gcgcgcttcc ggattggcga aatagtgacg
     9661 accaatgcgc gtatacagcc ctttcatccc gttaaggatc agaccgtaaa tcgggttgcc
     9721 ggaagcaaac gccaggccgc ggaatatgtt gtaatccagc tcggcaaagg catcggcgtg
     9781 atcggccact tcattagcgg tagccagcac ttcctgcgct ttatcgggat gctgacgaaa
     9841 cgcggtgcga ataaaaatag tggaaatatt ggtacgcacc gacagcaaat tatcaataag
     9901 ctgcggcaca ctttcgtgat ccagtcgcgc cagtgtttca aggatattta aaccagaggt
     9961 ttcccagaaa ttattcacct tcgtcggctt gccatgttga atggtcaacc agccatctcg
    10021 tgccagacgc tgtaacactt cacgtaacgt agtacgcgtt acgccaatta attctgaaag
    10081 ttcacgttct gcgggcaaaa tagtcccggg agggaagcgg ttattccaga tactttcaat
    10141 aatgtactct tccgcgaaac ccgccgggct ttgcgcctta atgaccatag tgagatttcc
    10201 ataacacagc aaaacaaagt tggactcatc ataccagagg gctaactacg ctgatagcag
    10261 acaaaaagaa gaaaaaggga tcaaggcttc attttccgca tctctttccc cttgactatc
    10321 aagacttgcc tcactcctgc ctgaccgtta agctttgcgt ttaaacataa aaacatcatt
    10381 gattatttgt aaggtaaggg aaaccatcat ggagatctcc tggggccgcg cgctatggcg
    10441 caattttttg taccagtccc ccgactggta caaactcgcc ctcatcattt tcttaatcgt
    10501 aaacccgtta attttcctca tcagcccttt cgtcgcgggc tggttgctgg tagcggaatt
    10561 tattttcact ctggcgatgg ccctgaaatg ctacccgcta ctccccggtg gtctgttggc
    10621 tatcgaagcg gtattcatcg gcatgaccag cgcggaacac atccgtgaag aggtggcggc
    10681 aaatcttgaa gtcttgctgt tattgatgtt tatggtggcg ggtatctatt ttatgaaaca
    10741 gctgttgctg ttcatattta cccgtttgct gttaagcatt cgctccaaaa tgctgctgtc
    10801 gctctctttt tgcgtggcgg cggcgttcct ctccgcgttc ctcgatgcct taaccgtcgt
    10861 ggcggtggtg atcagcgttg cagtcggttt ttatggtatt tatcaccgcg tagcctcttc
    10921 ccgtactgaa gataccgacc tgcaagacga tagtcatatc gacaagcatt acaaagtggt
    10981 tctggaacag ttccgtggct ttctgcgtag cctgatgatg catgccggtg tcggcactgc
    11041 attaggcggc gtgatgacca tggtgggcga accacagaac ctgatcatcg ctaaagcggc
    11101 tggctggcat tttggcgatt tcttcctgcg catgtcgccg gtgaccgttc cggttctgat
    11161 ttgtggcctg ttaacctgcc tgctggtaga gaagctgcgt tggtttggct acggtgaaac
    11221 gctgccggag aaagtccgcg aagtgttgca acagtttgac gatcaaagcc gccaccagcg
    11281 tacccgtcag gataaaatcc gtctgattgt ccaggcgatt attggcgtct ggctggtgac
    11341 tgcgctggcg ttgcatctgg cggaagttgg cttgattggt ttgtcagtca ttattctggc
    11401 gacatcattg accggtgtca ccgatgaaca tgctatcggt aaagccttca ccgaatctct
    11461 gccattcacc gcactgttga cggtgttttt ctcggtagtc gcggtgatta tcgaccaaca
    11521 actgttttcg ccaattattc agtttgtgtt gcaggcatcg gaacatgctc agctgtcgct
    11581 gttctatatt ttcaacggtc tgctgtcatc catttcggat aacgtcttcg tggggacgat
    11641 ttatatcaac gaagcgaaag cggcaatgga aagtggcgct atcacgttga agcaatacga
    11701 gctgctggcg gtcgccatta ataccggtac caatctgccc tccgtcgcta cgccgaacgg
    11761 tcaggctgcg ttcctgttcc tgctgacctc tgcactcgcg ccattgattc gcctctctta
    11821 tggccgcatg gtgtggatgg ccctgcctta caccctcgta ctgacactcg tcggcttgct
    11881 ctgcgtcgag tttacgcttg cccctgtaac cgaatggttt atgcaaatgg gctggatagc
    11941 aacgctttga taacaactta ccgggcatat aaatgcccgg tttgcctttt cgcccgataa
    12001 ttgtccaatt gcttcctatt tttgctgcct cctggtggcg cagcgaatga attggtttaa
    12061 actgcgcact ctatgcatat tgcagggaaa tgattatgtt gcgatttttg aaccaatgtt
    12121 cacaaggccg gggcgcgtgg ctgttgatgg cgtttactgc tctggcactg gaactgacgg
    12181 cgctatggtt ccagcatgtg atgttactga aaccttgcgt gctctgtatt tatgaacgtt
    12241 gtgcgttatt cggcgttctg ggtgctgcgc tgattggcgc gatcgccccg aaaactccgc
    12301 tgcgttatgt agcgatggtt atctggttgt atagtgcgtt ccgcggtgtg cagttaactt
    12361 acgagcacac catgcttcag ctctatcctt cgccgtttgc cacctgtgat tttatggttc
    12421 gtttcccgga atggctgccg ctggataaat gggtgccgca agtgtttgtc gcctctggcg
    12481 attgtgccga gcgtcagtgg gattttttag gtatggaaat gccgcagtgg ctgctcggta
    12541 tttttatcgc ttacctgatt gtcgcggtgc tggtggtgat ttcccagccg tttaaagcga
    12601 aaaaacgtga cctgttcggt cgctaatccc ttcagcgctc ctgcgggagc gcttttttcc
    12661 tgccgctata tttatttgac ccgcagtaaa tcagaacttc gcgttgtata acgtggtgaa
    12721 agcatggcac gcttcatctg ccattgttgc tggatcccct gcccggcaaa atagagtgtt
    12781 cctctgccct ctttagcatt cagtgtatcc attaccgtca tcaattgctc actcccgggg
    12841 cgcggtgcgt tgtcatcgaa taaattgagc tgcgcgactc cctgactgaa gaaatccccc
    12901 agcatcacgc ccgctttttg gtagcgatgg cccgcttgcc agatggcatc cagagatcgc
    12961 gtagcagcgt taatgatatc cctgctgtcc tgagtgggcg tcagcagttt taccgacgcg
    13021 ctattgccgt aataaggttc attgagcgca aatggtgacg tcttaataaa tgtggagata
    13081 aaccgacaat attgatgctc gctgcgaagt ttttccgccg cccgggcagc gtaactacaa
    13141 atggcctgcc gcatcgatgg ataatccgtg atgcgttcac caaacgagcg ggaacagata
    13201 atttcctgct tcgtcggtgc aaactcttcc agttgcaaac agggttcgcc gcgcagttca
    13261 cgcaccgttc tttcgagcac gacattaaaa tgtttacgga taaaccggat atctgtatcc
    13321 gccaaatcga gaacggtttt gatccccatc gcgtccagtt ttttgctgat ccgccgtcca
    13381 atcccccaga cgtcatccac ggggagagca gacattaatt tacgctggcg ttccagattt
    13441 gataaatcca ccaccccacc cgtctgccgc tgccattttt ttgccgcatg attggcaagc
    13501 ttagccaacg ttttggtctg ggcgatcccc acaccaacag taagatgggt acgttgtagc
    13561 accgttgcgc gaatttctct gccaaaatca gtcagatcgc gacaattacg cacacctgtc
    13621 agatcgcaga atgcctcatc aatactgtaa atctcgacgc ggggcgatag ttcttccagc
    13681 gtcgacatca cccgattgct catgtctgcg taaagctcat aattgctgct aaagcaaacc
    13741 acgccacagc gacgaaacag atctttttgt ttgaaccagg gatcgcccat tttaacaccc
    13801 agcgcctttg cctcagcgtt tcgggcgata acgcaaccgt cattattcga tagcacaacc
    13861 accggtttac cccataaatc agggcgaaac accgtctcac agctggcata aaacgcgttt
    13921 acatcacaga gagcaaacat cagcgcatcg ccttaacgac gtggatcacc acaccaaaga
    13981 catccagcgt atcttcgcta ctgatggtaa tgggcgagta cgcactgttc atgggaataa
    14041 gctgtaccgt cgggcgtagt tgcaattttt tcaccgtaaa ctcgccgtca acagcagcga
    14101 tgacaatatc accatggctg gcggtaatag cgctatcgac aatcagtaaa tcaccgtcac
    14161 taattccacc atcaatcata gaatcaccac ttgctttgac gaagtaagtc gcgctgggat
    14221 gctggatcaa cagttgattc agatcgatgc gctgttcaac gtaatctgct gccggtgaag
    14281 gaaagccaca ctgaacaaga tcgctaaata gcggaaaagt cacaatttcg cggagatccg
    14341 caggcttgat aaacaacata ataatctgcc tgaagttata ctgtttttat atacagtagt
    14401 ctgttcttgc cagcagatca atactgattc agactatcaa tatttgtcgc tgcatagact
    14461 gctgattttt cgttctctta tcctgtgctc acgtggcctt ctggcgacga cgctcatcca
    14521 gcagaaatga aaaatatcac ccggcaaaaa aatagaatag aagcattcgc cataatgaca
    14581 ttaaacattg tttgatattt atcatattaa tagaaataaa gacattgacg catcccgccc
    14641 ggctaactat gaattagatg aagtaaaatt tattaatagt tgtaaaacag gagtttcatt
    14701 acaatttata tatttaaaga ggcgaatgat tatgactgaa atcgttgcag ataaaacggt
    14761 agaagtagtt aaaaacgcaa tcgaaactgc agatggagca ttagatcttt ataataaata
    14821 tctcgatcag gtcatcccct ggcagacctt tgatgaaacc ataaaagagt taagtcgctt
    14881 taaacaggag tattcacagg cagcctccgt tttagtcggc gatattaaaa ccttacttat
    14941 ggatagccag gataagtatt ttgaagcaac ccaaacagtg tatgaatggt gtggtgttgc
    15001 gacgcaattg ctcgcagcgt atatttttct atttgatgag tacaatgaga agaaagcatc
    15061 cgcccagaaa gacattctca ttaaggtact ggatgacggc atcacgaagc tgaatgaagc
    15121 gcaaaaatcc ctgctggtaa gctcacaaag tttcaacaac gcttccggga aactgctggc
    15181 gttagatagc cagttaacca atgatttttc agaaaaaagc agcttttcca gtcacaggta
    15241 gataaaatca ggaaggaagc gtatgccggt gccgcagccg gtgtcgtcgc cggtccattt
    15301 ggattaatca tttcctattc tattgctgcg ggcgtagttg aagggaaact gattccagaa
    15361 ttgaagaaca agttaaaatc tgtgcagaat ttctttacca ccctgtctaa cacggttaaa
    15421 caagcgaata aagatatcga tgccgccaaa ttgaaattaa ccaccgaaat agccgccatc
    15481 ggggagataa aaacggaaac tgaaacaacc agattctatg ttgattatga tgatttaatg
    15541 ctttctttgc taaaagaagc ggcaaaaaaa atgattaaca cctgtaatga gtatcagaaa
    15601 agacacggta aaaagacact ctttgaggta cctgaagtct gataagcgat tattctctcc
    15661 atgtactcaa ggtataaggt ttatcacatt taaaaacaca gacgtttttt aaagattgag
    15721 agttacctgt tgataataag gtatcgggta actctcatta tgacagagcg ctttcaaaaa
    15781 cagggctacg aaatggcgag catccagacg tatattaaga aagcccccat caacatctat
    15841 taaatttatc actgcgccca gtcaggttta tttaagatat gatcctgcca gtcaatcact
    15901 tctgattctt tcactgcgat atgacgcaca gagatacgtt caccatgcat tgccgctttc
    15961 gaaccagtaa gtagcggatg ccacgcaggt aaatctttac cttccgccag caaacgataa
    16021 gcgcaggtca ttggcagcca ttcgaatgtt ggcagatttt cacgggttaa tttaatgcag
    16081 tcgggttcaa actcgaaacg acgttcgtag ttccgacatt gacaggtttt aatattgagc
    16141 tggcgacagg cgacgttagt gaagtagatt tcgtcggtgt cttcatccat cagtttatgc
    16201 aggcaacact gaccgcaacc atcacataac gactcccatt ccgcatcgct catttcgtcc
    16261 agggttttac tttgccagaa aggtacatcg ctcatcaggt gttccgccat tacgttaaaa
    16321 cgcaccttat aaccagtctg gcacagcgat gcaagttttg ccgccgcttt caggcggcaa
    16381 aaagtattac aaaacgcgag ttgccaaaga atgcccatcg aaagtgactg tcagctcatc
    16441 accgctttgc aacgggccga cgccatcagg cgtgcctgtc agcacaacgt caccggcctt
    16501 gagggtaaaa aacttgctca tataagcgat cagcggaaca attttatgga tcatgtccgc
    16561 agtcgtacct tgctgacgtt gctcgccgtt tacgctcagg cccagcgttg tattttgcgg
    16621 gtcgccggta aattccgccg cgggaataaa cccggaaagc ggacaagagt tatcaaacgc
    16681 tttagccttt tcccacggct gcccggcttt cttcattttt ccctgaacat cacgcaatgt
    16741 cagatcgagc gccacgccat aaccggcaat ggctttgcgg acatgctctt ccgtagcctg
    16801 acgcagcgtc gcgccaatca acaccgccag ttcgacttca tgatgaactg aaccgaaatc
    16861 ggatgggatc gccaatggct gccgcagatc gcacagtgcc gtttctggtt taataaacag
    16921 cactggctct tcgggcactg cgctgcccat ctctttaata tgtttggcat agttactgcc
    16981 aacacagact actttactca ccggataatc cagcagcgca ccttgccagt tgtgatgttg
    17041 atacataacg ttcccctcac cttatcgtgg atatgaaaac ccgcgccgac aaagataatg
    17101 ccaccgccgg atatcggtta tttgttagtg tcgtctgttt tctgccccat gacggaaaga
    17161 tgttgcttca gcaaatcttc gggtggtggc ggtaactgca aatagtaacc ttgctcggtt
    17221 aatgccagtt taaccttttc aatatcagca ttgaccagtt tcttccgccc atccaacggc
    17281 agaatcatcg ctaactgagg ctgaccaaaa cctttcatca gttcctcagg aacacgcgaa
    17341 aaatcgtctt ttttttcgac atataaatag gtctggtcac gcttgctgct tcgataaatc
    17401 acacaaaaca tacttttact ctttaagatg ccaggttttg gcataagcta acatcaatga
    17461 aacccgcgcc acaagccagc cagctgtaga tttctacggt ttgttgagtc catgcccctt
    17521 tataagtaat ccccccggca aaaaatgccg cgccgggatt ttaccgaacg cagaaaagcc
    17581 actggaattc tcccgtaagg gattgttata ttataacagt tcatcgtact cattctgaac
    17641 aggagactac caatgaaaat caagagcatc agtaaggctg tattgctgct tgcactgcta
    17701 acctccacga gctttgctgc gggtaagaat gtcaatgttg agttcagcaa aggccatagc
    17761 agcgctcagt actccggcga aataaaggga tacgattacg atacatatac cttctatgcc
    17821 aaaaaaggtc agaaagtgca tgtgagtatc tctaatgaag gcgctgatac ttacctcttc
    17881 gggccaggaa tcgatgattc agttgattta tccagatatt caccagaact cgatagtcac
    17941 ggacaatact cactccctgc ttctggcaaa tatgagctgc gagtgctgca aacccgaaac
    18001 gacgcccgta aaaacaaaac taaaaaatat aacgtcgata ttcaaattaa gtaaatgtca
    18061 accgacgggg actctccccg tcataaacgt taacgcccca ctcctctctg tcgctcaaga
    18121 ggttaatgac gaatagcaga caatgccgaa tagaacataa tgtaaacaag gcatcactca
    18181 cgacacataa ataacatctt tgtggtgatt tgattgattt ttccactccg cttaccatcc
    18241 acaccaaaat aacgatcatc acgacataat ttttctttaa catcgcaaaa tacaccgtta
    18301 gcgaatgtaa attcgctggg atcgtagcct tgtaatgatt gtaagttttc agcggctttt
    18361 tcgccgagat atttccttgt taactctggg gaaatgccat ctgagttcac acaaatatac
    18421 gggtcgcaaa caacgccgca cgccggagat tgaagtccgc cagcgtcatg tccagacgcc
    18481 attgatgaga acatcatccc cacacctgac aatccgatat aaaaaatacg catcatattc
    18541 atagcacatc ctgataatcc aaccgtacaa cacttatgct catttcatca tcaatccgta
    18601 atcaaaaccg ctgacctgga cgagttacga tgacctgaca gaagtatcag acgtcttctg
    18661 cggtttctat gatagagcaa ttctgtggcg gatattaaga taacgggtat ctgttcttta
    18721 atgatgccgc aaaaaatgga aagttaagaa agcctggcgt ggtgaaaaaa tacacttgcc
    18781 tcaatactgg atatttaaac aggtctggag atgctgtcac gattagggtg cggaaaaagg
    18841 aagcgtaaat aagaacaatc aaaagtacga cggcaatggg ttgattgaca agggtatttt
    18901 ttaagctatg aatcagcgcc atttatcaca gaatagactt ttactctgaa taaatgggag
    18961 ggtgacttgc ctcaatataa tccagactat aacatgcctt atagtcttcg gaacatcatc
    19021 gcgcgctggc gatgattaat agctaattga gtaaggccag gatgtcaaac acgccaatcg
    19081 agcttaaagg cagtagcttc actttatctg tggttcatct gcatgaggca gaacctaagg
    19141 ttatccatca ggcgctggaa gacaaaatcg ctcaggcccc cgcattttta aaacatgccc
    19201 ccgttgtact caacgtcagt gcactggaag acccggtaaa ctggtcagcg atgcataagg
    19261 cggtttcggc aaccggtttg cgggttattg gcgtaagtgg ctgcaaagat gcgcaactta
    19321 aagccgaaat tgaaaagatg gggctgccta tcctgacgga aggaaaggaa aaagcgccgc
    19381 gtccagctcc cgcaccacag gctccagcgc aaaatacaac gccggtcaca aaaacgcgtt
    19441 taatagatac cccggtgcgt tccggtcagc gtatttatgc tccacaatgt gatctgattg
    19501 ttacaagcca cgttagcgct ggggccgaat tgattgccga tgggaacatt catgtctatg
    19561 gcatgatgcg cggtcgtgcg cttgcagggg caagtggtga ccgggaaacg caaatatttt
    19621 gtacgaacct gatggcggaa ctggtgtcca tcgcaggtga atactggctg agtgatcaaa
    19681 tcccagcaga attttatggc aaagcggcgc gactgcagtt agtcgaaaac gctttgaccg
    19741 ttcaaccgtt aaattgatcc ctttttaaca aggaatttct atggcacgca ttattgttgt
    19801 tacttcgggc aaagggggtg ttggtaagac aacctccagc gcggccatcg ccactggttt
    19861 ggcccagaag ggaaagaaaa ctgtcgtgat agattttgat atcggcctgc gtaatctcga
    19921 cctgattatg ggttgtgaac gccgggtcgt ttacgatttc gtcaacgtca ttcagggcga
    19981 tgcaacgcta aatcaggcgt taattaaaga taagcgtact gaaaatctct atattcttcc
    20041 ggcatcgcaa acacgcgata aagatgccct cacccgtgaa ggggtcgcca aagttcttga
    20101 tgatctgaaa gcgatggatt ttgaatttat cgtttgtgac tccccggcag ggattgaaac
    20161 cggtgcgtta atggcactct attttgcaga cgaagccatt attaccacca acccggaagt
    20221 ctcctcagta cgcgactctg accgtatttt aggcattctg gcgtcgaaat cacgccgcgc
    20281 agaaaatggc gaagagccta ttaaagagca cctgctgtta acgcgctata acccaggccg
    20341 cgtaagcaga ggtgacatgc tgagcatgga agatgtgctg gagatcctgc gcatcaaact
    20401 cgtcggcgtg atccctgagg atcaatcagt attgcgcgcc tctaaccagg gtgaaccggt
    20461 cattctcgac attaacgccg atgcgggtaa agcctacgca gataccgtag aacgtctgtt
    20521 gggagaagaa cgtcctttcc gcttcattga agaagagaag aaaggcttcc tcaaacgctt
    20581 gttcggagga taagttatgg cattactcga tttctttctc tcgcggaaga aaaacacagc
    20641 caacattgca aaagaacggc tgcagattat tgttgctgaa cgccgtcgca gcgatgcaga
    20701 accgcattat ctgccgcagt tgcgtaaaga tattcttgag gtcatttgta aatacgtaca
    20761 aattgatcct gagatggtaa ccgtacagct tgagcaaaaa gatggcgata tttctattct
    20821 tgagctgaac gtgaccttac cggaagcaga agagctgaaa taagcccgct gtaaaaacgc
    20881 atttatcttc aaggcagagt catttctgcc ttgagttttt catccctctc atccacgttg
    20941 tggtaaagcg gcgagtattc ttgctgatac tcctcattgc tatttccacc tcccccttta
    21001 caccttaagg ctgttcccac gcacagaaca aaatgtttat gcctggttga gtaaataacc
    21061 ctgttgttag tgtggatata ctgggagatg ataaccccgc aatttcatta ttcattgttc
    21121 tcccatagga tgaggtttcc cgccgtaact ggcgggcttt ttttgcccca gaattttctc
    21181 tttcaacatc ctgtaaacga aaactgcgcc gaagcgcagt ttaatcgttg atggtcagaa
    21241 cgaccagttc acaccagcca ccgcgttcca cggggactcc acaccggcac catggctata
    21301 ccccaccccg aaatgcccgc ttaacgtact gctgaatgag gctttaatac ctgcctggta
    21361 tattccacgt ctgcccgaca aatcattgac aaaattaccg tcactattca ctttcacccg
    21421 attatcatcg acaaattctt tgcgcacagc cgccttcagg cagggttcaa tttccatacc
    21481 gttccccaga cgcatgttgt aactcagcgt tgcacccagt tcacgatata tactgcgggt
    21541 atcgactgat ttcgattcca tgccattgga taaatgatag tagcgtctaa gtgacctaag
    21601 ctctgctgca acatcagccc gcaggcacca gaccagggga ttcatcctga agagacagcg
    21661 caggtgtatt gtgttcaccg ctcaccaaag acatcatgat gaaatgatga tatcccgcat
    21721 aagaaagagg cattttttac acgcagtgcg ctgaagtgta gttggataaa aaatcactct
    21781 tttcagataa tactgtcgta cgcttatctt tcgacaatga ggccgtcggc aaaataaaat
    21841 gactaatgta atcgttgctg atgaatatct tctgctcaca taaaaatcac acaatgatat
    21901 tgataaatca gattatctta cctttacaac attgcgttaa cagtcaatga cacttcttga
    21961 ataaaaatat tgtcaatcaa tgagttattc ctcatttaag attattctca ttcacgtaaa
    22021 aaacataatt cctatcatca cagccacgct cattttgaat tatctgaata atagcgagcg
    22081 ttctttagtt aagcagatct taattgaaga cgaaattatt gtgtgtgcaa cttacttaat
    22141 accggacatc taagttgaag aaaaaaatac ttgccttcgg gcttatctca gcattgttct
    22201 gctccacgcc agcgatggcg gatatgaacc gcaccacgaa aggtgcattg ctaggcgcag
    22261 gcgttggttt acttactggc aatggtgtta atggcgtact gaaaggtgca gctgttggcg
    22321 ctggtgttgg tgcagtaaca gaaaaaggcc gcgacggtaa aaatgcgcgt aaaggtgcaa
    22381 aagtgggtgc cgccgttggt gctgtcactg gcgttctgac cggcaatggc ctcgaagggg
    22441 cgattaaagg tgccgtgatt ggcggtacgg gtggtgcaat tctgggtaaa atgaaataag
    22501 gaatgatgat gaaaaaattt gctttactgg cgggcctgtt tgttttcgca ccaatgacct
    22561 gggcgcagga ctacaatatt aaaaacggtt tgccgtcaga aacctatatt acttgcgcag
    22621 aagccaacga aatggcaaaa actgacagcg cacaggttgc agaaattgtt gcggtaatgg
    22681 gtaatgccag cgttgccagc cgtgatttaa aaattgagca atctccggaa ctgagcgcca
    22741 aagtggtaga aaaactgaat caggtttgcg ctaaagaccc gcaaatgctg ttgatcacgg
    22801 caattgatga caccatgcgt gctatcggca aaaaataata tttcatatgt accgcgtcga
    22861 tgaatacgac gcggtttttt agctaacggc gggatcagaa cttatatttc aggcccacca
    22921 tcactgcggt gtcattgtag ccattatcac ccagctgcac gcccacattc ccccagacgc
    22981 tggccgctgg attcaggttg ccatttacgc caagtttaat ttcccccaaa ttactcgccc
    23041 catcttgttc gactttcaca ccgttcattg agacagcaaa atctttactg ttatggatcc
    23101 agttcatttc aacaaaaggt tcaaagtcgt caccgtgcgg accgctgttt ttctcctgag
    23161 tacgaataaa ggtgcggaaa ccaagtcggg tctggatgtt gttatttgcg tcattctcaa
    23221 tacgtgagcc attggcttcg gtgtgcgcat tatgatcgac gcccatccag atagcctgtg
    23281 cctgtggctg gaatatccac gtgtatttcg ccgcttccag accaaaggat ttattcaggg
    23341 caaagccgta acccgcttcc agcgagacgg ttgcgccttt cgcactatag gattcatagg
    23401 ctaattcatc ccctttcacc gagtttttaa accagctata ttgcgcccag gcgtcaatgt
    23461 atgcgccttt tttactgatg tcatcggcaa accaggtggc atacagccct gcgcaatagc
    23521 ctctgacact ccctttcgaa cgataatccg acacgctgga atgagttgag ttgtagtcgc
    23581 gggcataacc agccatcact cccaaacgcc aactgtcact gtcggtaaaa ccaccggtta
    23641 acagatcggc ccctaactgc gagacgtagc gatgcgaggt cgttctcaac tgtccgttgc
    23701 tgtcacgcca ggcattatgc ccgccaattt gacgtagcca aagtcggctt gaacgctcct
    23761 gttcagttac aggatcgatg tagcgcattt cacccgcacg gtcgttcaga cgcataacaa
    23821 aaagagtgtt agcggctgcc aggttggcga cgtaacttcc agcttcgagg cgcaggacgg
    23881 gggtaggcgc aacagtaggc gctgaacttc ctccggagtt tgtttcaccg ccgctcgttg
    23941 cctggaagtt agtcagatac cagtttttat tggtcgcttc ggtattacct cgggttaatg
    24001 aatattcgta ggcaccggcg aaaatcctcg aatccttaat aaattcccca tttgattccc
    24061 cctcaacgct gataatctct atcccattta acgtgtaagc tccgctgcct cctgcattgg
    24121 taactttaac tcgggttgtt ccagaggtat ttccattaat aacgacttta tccgtagctg
    24181 aattatcatc acccaattca gttctgagat gcaatacgcc attattacca acgtaatttt
    24241 cagttattgt taatcttgtt ggttcgacat cccgtccatc tgcccgggaa atatagaccg
    24301 tactattatc aaccgtgagg ttgctcaccg ttgagtcagc tttaatatcc caggtactat
    24361 tatctgaaag cgacagatag gtatgagtgt taacatctgt tgaaatatta gctgaacccg
    24421 ttatttttga attagttgca tcgacggtca aatgaccttt aatattattg ataaaaacat
    24481 ctgcagccga tgatacctca ctatccgtaa tggtcacgtt actcgtctca tttttatggt
    24541 agaggatttc atttgctgac cgtagtgagc taccagataa aatatctacc gttgcttcct
    24601 gaccatttat ggaagactcc agcacaattc cagacccctg atgcgcgact gcagatgtat
    24661 ttttcagcgt caccgtgccg ccattataaa ttttagcagt cgcggcgtca ggacccgttg
    24721 tataaatttc tccctggtta aatgaaactg tcccctggcg ggcagcgatg gcatgagcat
    24781 tgtccccatg cgtggtgata tcaacatcgg ttaatgtaac gctggcattg gtataatcaa
    24841 gcgtgtcaac agccttgacg acagtgccat tagcgtacgc ggcataactt tctgttccgt
    24901 gggttgtgat ctcccctttt gccatatcca gttttgctat ggcattattt tcattacttc
    24961 ctgtatcggt agtactttct tcatcaagag aagatacaca cgcacgcgcg cttccgggag
    25021 atgcacacgc aagaacgccg actgcgtgat tacccgcagt aacaataaat gtatcatctc
    25081 cgtcagaaaa agagggggta accgaaccgt cagagttgac tcttattatg gtgtcagtat
    25141 tcaggtttcc actcgtttga cttagcaaac caattgagta atcccccgtt atgacaatcg
    25201 tgtttttacc ctgcaaaata acagcaccgc caaattcact tacgattgcg ctagctgtta
    25261 atgctgtgct tagaggacta ttgtcagaag gatataaacc gcccgttgtt acaatgacgc
    25321 cattgttaat ggttaacttg ctaccctctc ctgcaacgga tacccgtaac ttccattacc
    25381 cgttgttata actagcgcat tatcaatatc ttttactttt ccatctttta ttgccacagc
    25441 atgtttatat tcatcgtgct gaataaactg ttcagcatat gcattaccca ccgccagagc
    25501 aataagtccc aaactaaacc cggggagttt tttcagtttc atagattaac catccattca
    25561 ttggattaac aaacattcct ggtcaaataa cctatgagct cagattacca aaacggaggg
    25621 taaagacaaa aacaaaagca caaaaaattt aaatagataa acagcgacaa tatataaatg
    25681 ataaataagt cggcattatc atttttcacc ttattttcat gacattgatc actttgaggt
    25741 gacgattttg tttaacaaat aactcttcga catcacaatc attgatagtt gcaataaaaa
    25801 ttatttcaat gacatataaa aacaactgtg ataacaccag ggttgacaac tatttgcgca
    25861 ataattcctt tacttcaatg atccaggcat tcactgccag caacccattc agccgcatac
    25921 tgacagtgag aaactgaaag attattgctg atccctgtgg ctgacgagtc tttcccttaa
    25981 tcgattaacc agattgatcg ccagccaaac aagggcatta agcaaattta tcgtcacaag
    26041 cacttctccc agcccggtac tgggttttgc aataaggatg accaggaggg tgatcagcat
    26101 aaatgcaatg gaaaagatga tatagagagt gaaataatcc agattattac tattgttcat
    26161 gctactaatg gcaatcctgt caaaactttc aaacaattat agcaaacaaa ataatgaaca
    26221 atagtaacaa gaggcaacga gctaattagc tgtctataat tacaccgcac tatggcctta
    26281 ctgatcaatt ttcactcaac cacaaccttc accttcggtt tagaaaggat aattttgacc
    26341 aggtcgatgt atgtaacagg tttatagaaa taataaccct gctgaaatgt gatattattt
    26401 tggttcagat agtcaagttg ttctttcgtt tcgacacctt cagcgacgat actgatcgaa
    26461 agtttacgcg ccagatccaa tacacaatcc agaattcggg ttgattctgg gtctgcgttt
    26521 acgcggccaa cgaaactgtg atcaattttg atataatcaa tatgcaggtc atgaagataa
    26581 gaaagccctg agtagccagt accgaaatca tccagcgcga tgacaaaacc attttcatgc
    26641 agaatgttca accgctgaac cagactttca tcaacattca atggctcacg ctcggtgact
    26701 tccagaacaa ggtttaaatc gcggcgggta aaactgtcac ggaaatttaa acactcgtcg
    26761 acaaacgtcg gcgaaataat atgcgaagcg ctaaaattaa ttccaatatg aaaaccttcc
    26821 ggcaatttac tcgcgatagc gttcatctgt ctggcaacct gattcatcag gctttgcgta
    26881 agcggaacga ttaatccgga tttttcagca agtggaataa atgccgcggg tgatatatat
    26941 ccaccgtgag gttgtttcca gcgggctaac acctcaactc cccgcaatgt cccttcccga
    27001 ccatttacca caggttggta aaaagggacg atctccccat tatttattgc ccgtcgcagg
    27061 atctcttcag gcgttgcgct tttattgaaa tatctatcaa gcaggaaggc agcggcgcag
    27121 gcgattaaca gaataaatat taaaatccca aagcctttat cgataagtct gtagaagcta
    27181 aagagtggcg gcgggttaaa gataatagtg aaatgatatt ttgtggagtg aaccttctcg
    27241 gtatagggat aatgtccatt aagcgtcatc acatcacccg ttggtccaat aatgtcatcc
    27301 gccacgcgta atacatacct tacccctttc aagggtacat taagtgcccc acgaatatgc
    27361 tgatcgctta tcgtaaccaa aatgcgcgta tctgcaaatt ggttctgata gagcaggata
    27421 ggtaatctat taacggtgtc gattgctgga gccaacagta aattactgtc cgggaaaaca
    27481 ggaatacgag acaacaggac ccgattccca ggcagggatg tacaccagac tattccctgt
    27541 ttgagaatga tgattgtgcg caggtgaggt ttcagagctg cttcagtgcc aagctgatat
    27601 tgctcctcga ggttgcattc cttcccggca atgttaaccg ctgtttgagt cgcttgtgac
    27661 gcttcttcaa gtatatgatt gatattgttg gcggcatatc tcgctcccgc caggtactct
    27721 gcttttgcgg aataccagag ctggatgttc aaaatagcga caccggtaat aaacaagcat
    27781 atcgcaacga ggatgggtat gagtgtattg cgcatttgct tgtcccggcc aaagggttta
    27841 atacatgaaa atgccattca ttcgctcgcg ctggcatcta cagagagcat ggtagagagc
    27901 ataagaaata tccacgccga agataaaatt ttctccatct tgatctaacc cattaattat
    27961 tgagcataat gtaggcagta caaaataagt taggcgggat atcaggcgtc aagaatggag
    28021 agcgagaact ctccattctt gacacctgat attgcggaca taataagaaa gcataacgcc
    28081 tgaaatgctc actttgcatc agcatggtga tacagctgat gtttattcta aaaccttact
    28141 caagttctaa gagagcacgg attccctgtc attaataatg atatccgtta gtgtagtctt
    28201 atgcatttca atgtactgca atatttgcag atagagatca gaaagaatca cactggtttc
    28261 atcttttaaa cgcaagcgta aagcctgatc aataatatga ctatcaacat acccatgcgt
    28321 catgaacacc tccctctcga gatttttaaa caaagcacat tgaaaaataa atctctctgg
    28381 gattgaatta ttcatgtcag cctcgtagaa atattaaccc aataa
//
LOCUS       DAZFQD010000058        27793 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.058, whole genome shotgun sequence.
ACCESSION   DAZFQD010000058 DAZFQD010000000
VERSION     DAZFQD010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27793
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.058"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            25..687
                     /gene="acrS"
                     /locus_tag="ACHKTW_002542"
     CDS             25..687
                     /gene="acrS"
                     /locus_tag="ACHKTW_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrS"
                     /protein_id="HFO6463545.1"
                     /translation="MAKRTKAEALKTRQELIETAIAQFAQHGVSKTTLNDIADAANVT
                     RGAIYWHFENKTQLFNEMWLQQPSLRELIQEHLTAGLEHDPFQQLREKLIVGLQYIAK
                     IPRQQALLKILYHKCEFNDEMLAEGVIREKMGFNPQTLREVLQACQQQGCVANNLDLD
                     VVMIIINGAFSGIVQNWLMNMAGYDLYKQAPALVDNVLRMFMPDENITKLIHQTNELS
                     VM"
     gene            complement(690..869)
                     /locus_tag="ACHKTW_002543"
     CDS             complement(690..869)
                     /locus_tag="ACHKTW_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HFO6463546.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLATETDKCRNMNSVNP
                     LKLVNCDELNFQDRM"
     gene            complement(953..1837)
                     /gene="yhdJ"
                     /locus_tag="ACHKTW_002544"
     CDS             complement(953..1837)
                     /gene="yhdJ"
                     /locus_tag="ACHKTW_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417728.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HFO6463547.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDALAELKKIPAESVDLIFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPEALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAVSSGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            complement(1923..2219)
                     /gene="fis"
                     /locus_tag="ACHKTW_002545"
     CDS             complement(1923..2219)
                     /gene="fis"
                     /locus_tag="ACHKTW_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HFO6463548.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            complement(2245..3210)
                     /gene="dusB"
                     /locus_tag="ACHKTW_002546"
     CDS             complement(2245..3210)
                     /gene="dusB"
                     /locus_tag="ACHKTW_002546"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFO6463549.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSDPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            complement(3539..4420)
                     /gene="prmA"
                     /locus_tag="ACHKTW_002547"
     CDS             complement(3539..4420)
                     /gene="prmA"
                     /locus_tag="ACHKTW_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFO6463550.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            complement(4432..5883)
                     /gene="panF"
                     /locus_tag="ACHKTW_002548"
     CDS             complement(4432..5883)
                     /gene="panF"
                     /locus_tag="ACHKTW_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015233 - pantothenate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015887 - pantothenate transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0036376 - sodium ion export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HFO6463551.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIVVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            complement(5873..6115)
                     /locus_tag="ACHKTW_002549"
     CDS             complement(5873..6115)
                     /locus_tag="ACHKTW_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HFO6463552.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVAAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            complement(6224..7573)
                     /gene="accC"
                     /locus_tag="ACHKTW_002550"
     CDS             complement(6224..7573)
                     /gene="accC"
                     /locus_tag="ACHKTW_002550"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFO6463553.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSADRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(7584..8054)
                     /gene="accB"
                     /locus_tag="ACHKTW_002551"
     CDS             complement(7584..8054)
                     /gene="accB"
                     /locus_tag="ACHKTW_002551"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFO6463554.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            complement(9032..10006)
                     /locus_tag="ACHKTW_002552"
     CDS             complement(9032..10006)
                     /locus_tag="ACHKTW_002552"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family oxidoreductase"
                     /protein_id="HFO6463555.1"
                     /translation="MQALLLEQQDGKTLASVQTLDESRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASRILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            10158..12098
                     /gene="csrD"
                     /locus_tag="ACHKTW_002553"
     CDS             10158..12098
                     /gene="csrD"
                     /locus_tag="ACHKTW_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HFO6463556.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSTLRPQITELMMSADIVRVDLLHGDKQVYTLARNGSYRPVGSSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIIVMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIAGQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTQVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            12403..13446
                     /gene="mreB"
                     /locus_tag="ACHKTW_002554"
     CDS             12403..13446
                     /gene="mreB"
                     /locus_tag="ACHKTW_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HFO6463557.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            13512..14615
                     /gene="mreC"
                     /locus_tag="ACHKTW_002555"
     CDS             13512..14615
                     /gene="mreC"
                     /locus_tag="ACHKTW_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFO6463558.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGVSQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPTTGNAATA
                     PAVPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            14615..15103
                     /gene="mreD"
                     /locus_tag="ACHKTW_002556"
     CDS             14615..15103
                     /gene="mreD"
                     /locus_tag="ACHKTW_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFO6463559.1"
                     /translation="MASYRSQGCWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSTLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            15112..15705
                     /gene="yhdE"
                     /locus_tag="ACHKTW_002557"
     CDS             15112..15705
                     /gene="yhdE"
                     /locus_tag="ACHKTW_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HFO6463560.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADTIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            15695..17164
                     /gene="rng"
                     /locus_tag="ACHKTW_002558"
     CDS             15695..17164
                     /gene="rng"
                     /locus_tag="ACHKTW_002558"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HFO6463561.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            17232..21032
                     /gene="yhdP"
                     /locus_tag="ACHKTW_002559"
     CDS             17232..21032
                     /gene="yhdP"
                     /locus_tag="ACHKTW_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709041.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HFO6463562.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLDAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGSDDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSTLKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTSVLPEAVNEALSGSVAWDGKVGIDLPYHAGATYNVEMNGDLNNV
                     SSHLPSPLAKPAGEPLAVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTPSSPFPTTERINFRGWPDAQIRCTECWFWGQKFGRIDSDITISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPENERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGDITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            21188..22633
                     /gene="tldD"
                     /locus_tag="ACHKTW_002560"
     CDS             21188..22633
                     /gene="tldD"
                     /locus_tag="ACHKTW_002560"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HFO6463563.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVSGTA"
     gene            complement(22761..23690)
                     /gene="aaeR"
                     /locus_tag="ACHKTW_002561"
     CDS             complement(22761..23690)
                     /gene="aaeR"
                     /locus_tag="ACHKTW_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HFO6463564.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSVNLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            23873..24076
                     /gene="aaeX"
                     /locus_tag="ACHKTW_002562"
     CDS             23873..24076
                     /gene="aaeX"
                     /locus_tag="ACHKTW_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump operon protein
                     AaeX"
                     /protein_id="HFO6463565.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            24084..25016
                     /gene="aaeA"
                     /locus_tag="ACHKTW_002563"
     CDS             24084..25016
                     /gene="aaeA"
                     /locus_tag="ACHKTW_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HFO6463566.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQILFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSNLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            25022..26989
                     /gene="aaeB"
                     /locus_tag="ACHKTW_002564"
     CDS             25022..26989
                     /gene="aaeB"
                     /locus_tag="ACHKTW_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HFO6463567.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            27081..27353
                     /locus_tag="ACHKTW_002565"
     CDS             27081..27353
                     /locus_tag="ACHKTW_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HFO6463568.1"
                     /translation="MNIYTFDFDEIESQEDFYRDFSQAFGLAKHKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(27409..27672)
                     /gene="yhcN"
                     /locus_tag="ACHKTW_002566"
     CDS             complement(27409..27672)
                     /gene="yhcN"
                     /locus_tag="ACHKTW_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HFO6463569.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDIREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
BASE COUNT         6664 a         6956 c         7396 g         6777 t
ORIGIN      
        1 tatttatttc ctgaataatt aatcatggca aaaagaacca aagccgaagc tctgaagacc
       61 cggcaagaac tgattgaaac tgccatcgcc cagtttgcgc agcatggcgt aagcaagacg
      121 acactcaacg acattgccga cgctgctaac gttacgcgtg gcgctatcta ctggcacttc
      181 gaaaacaaga ctcaactgtt taatgagatg tggttgcaac agccttcatt gcgggagtta
      241 atccaggaac acttgacggc tggattagag catgacccgt ttcaacaatt gcgtgaaaaa
      301 ttgattgtcg gcttgcaata tattgccaaa attccccgcc agcaggcgtt gctgaaaatc
      361 ttatatcaca aatgtgaatt taatgatgag atgctggccg agggagtgat acgcgaaaag
      421 atgggcttca atccgcagac tctccgcgaa gtattgcagg cgtgtcagca acaaggttgt
      481 gtagcaaata acctcgattt agatgttgtg atgattatta ttaatggtgc cttcagcgga
      541 attgttcaaa actggttaat gaatatggcg ggttatgatc tttataaaca agcccccgct
      601 ctggtcgata acgtattaag aatgttcatg ccagatgaaa acataacgaa attaattcat
      661 caaacgaatg aattaagtgt catgtaaatt cacattctgt cctgaaaatt cagttcgtca
      721 cagttaacca gttttagtgg attaactgag ttcatattgc ggcatttgtc tgtttctgtt
      781 gccagcagtt caatccactg catcagtaaa gtatcaaaca gaaaaacgaa gacagcgaaa
      841 acgacgagcc accaatactt gcgaatcatt acctgagcct gtgtatgttt aaccaagtct
      901 ggcaggaaat atacacgaaa tgaataatct gaaaaccagg cttacacttc ctttactttg
      961 caatgagatc ggggtcaact tcgcttaacc ggcttcgttt tgacaggttg ccggtctttc
     1021 tttttttcac tttcgccagt tcttccgcag agtaatgcga cgcgacatcc agccgtcgaa
     1081 gccccatttt gatgtactcg ctgttgatct caataccaat gaattttcgc ccgctggaga
     1141 cggctacggc accggtagta aagctaccgg caaacgggtc gagaacgata tcgcctgggt
     1201 tggaagaggc gagaataatg cgtttcagta aggcttccgg tttttgcgtc gggtggttct
     1261 catattcatc catcaaataa cgcacgcgcg gaaaatccca gacgttaccc ggtacttttt
     1321 gatgattgta tggctgtgga ggatttttgc gataatcgat caacgcgcgc tgcgatccgg
     1381 ttttggcttc taccagaata gcatcaccgt tgaatgtgta gttctttgcg tctttcacca
     1441 tcatcaggat gggttcgtac atggagccgt agtgtttttt cgcctgtact ccagaactgt
     1501 catatgacca gacgatgcga cttttgatgg taaaaagctt gcggcactgg agatcgataa
     1561 agggcatgtt ttccgtgctg ttcatgatgt acatgctgcc ctgctttttc agaacgcggt
     1621 ggcactctgc aatcacttca aacagccagt cgataaacag atcttctttc caggcttcga
     1681 tcagaccatc aaaattttta ccgatattat atggtgggtc ggcaaagatc agatcgacac
     1741 tttcggcggg gatcttttta agttcggcaa gcgcatcacc gtgaataatg gtcttagctt
     1801 cattaccaaa ccgggtcggt tcacatcctg ttctcatggt cactcccttt gtgacaccta
     1861 taaaaaaggc gcttccccat gccgagtagc gcctttttaa tcaagcattt agctaacctg
     1921 aattagttca tgccgtattt tttcaatttt ttacgcagcg taccacggtt gatgcccatc
     1981 atcagcgcag cacgggtctg gttaccacgg gtgtattgca tcaccatgtc caacaggggc
     2041 tgttctactt cagccagtac cagctcatag aggtcattca catcctgacc attcagttga
     2101 gcaaaatagt tcttcagtgc ctgtttaacc gagtcacgca ggggtttttg ggttacctga
     2161 tcctgagagt taacggtaga aacggtcagt acgtcagaat ttacgcgttg ttcgaacata
     2221 gttctgtcag ctctttattt ctgtttacgc aaaattttcg aagtatgcct ccaacgcctc
     2281 cagctgttcg ctggcatcct caatggcgtt gaatgtgcgc cgaaactggt catttggagc
     2341 gtgttcctgg agataccagg aaacgtgttt acgtgcaatt cggtaccctt ttgccggacc
     2401 ataaaagtca tgcagttccc gaacgtgcgc gcaaagcaag cgcttaacct ctgccaaagg
     2461 cagcgggggc agcaactccc cagtgtccag ataatgctgg atttcccgaa agatccaggg
     2521 tcttccctga gctgcgcggc ctatcatcag ggcatccgcc cctgtatagt cgagcacagc
     2581 tctggcttta agcgggtcag taatgtcgcc attcgcgata accggaatgg aaactttctg
     2641 cttaactgcc cgaatactgt cgtactcagc ttctccattg aacaaacagg cgcgtgtacg
     2701 gccatgaatg gtcagagcct gaatgccaca gtcttcagcc agttgggcaa tctcttcgca
     2761 gttacggtgt tccggtgccc agccggtgcg aatcttcagg gtaacaggaa cgtccactgc
     2821 attgacgacc tcggtaagga tcgatttaac gacatccggg tactgcaaga gggctgaacc
     2881 tgcgagcttg cgattcactt ttttagccgg gcaacccata ttgatatcaa taatctgggc
     2941 accgctttcc acgttaatac gtgctgcatc tgccatttct ttcggatcgc taccagcaat
     3001 ttgcacggtg cgaataccgg gttcatcaat gtgcaccatc cgtaaacgag atttgtcgct
     3061 ttcccaaacc tgtgggttag aagacatcat ctcggatact gtcaatccgg ctcccatctc
     3121 gtagcacaac gtccgaaaag gtctgtctgt aatgccagcc atgggcgctg cgatcaggcg
     3181 atttctgagc tgatattgtc cgatgcgcat gagttaagaa atgaccatac tgtgactgca
     3241 aggcggcgta tattacgcat tttttgcacg agatgaaagg ccaaactttg accaatcctc
     3301 tgagatggat caaagaattg catttaaaat gagcgtggtg cgataattac tcataaaaat
     3361 catcatatta gaaaatagtg actaaaattt acactcaaag aaatttgagt aagttctcaa
     3421 tttttcttta tgaatgaaaa ttttggcacg caaatttgcg taaataatcg gcaaatttac
     3481 gtgccttttg tgagcttgct cgcacttcgc cccgcgtcac cctacggcga tgcgaaggtt
     3541 aattcttacg accggtaata cggcaccact cttctttttc cacgaccggg tccagtgcga
     3601 agctgtcggc ataagcttca caaacgctct ctgcctggct tgccagaata ccggaaaggc
     3661 ccagcaaacc gcctgaaacc ggcaggacgc tgattaacgg tgccagttca cgtaatgggc
     3721 ctgcaaggat gttagcgacc accacgtcgg ctttcatttc ttctggctga tctttcggta
     3781 agtagagttc cagacggtca gaaacgccat tacgttcggc gttgtcgcgg ctggcctgaa
     3841 tcgcctgcgg atcgatatca ataccaatgg cttttgctgc acccagtttc agcgccgcga
     3901 tcgccagaat gccggaacca cagccaaagt cgatgactgt tttaccggtt aaatcgaggc
     3961 tgtcgagcca ttgcaggcac agagaggtgg ttggatgggt acccgtacca aacgccagcc
     4021 ctggatctaa catcacgttg acggcgtttt cgtccggcac atcacgccag ctagggcaga
     4081 tccacagtcg ttcaccaaag cgcatcgggt ggaaattatc catccattcg cgctcccagt
     4141 ctttatcttc tagttgttcg attttatgcg cgaagcctgc gccgagcagc ggatggtttt
     4201 ccagaatcgc caccacgtcg ttcatatcgg tttcagcgtc aaacagacca atcacatcgg
     4261 tgtcgcccca caggcgcgtt tcgcccggca gcggctcaaa tactggcgta tcgtgggtat
     4321 cctgaaaagt gatagaaacg gcacccgctt ccatcagcgc atcgctaaga tcttccgcgt
     4381 tcgcgccggt ggtgttcagt ttcagttgga tccaaggcat ggcaaaactc tttatttatc
     4441 agtagtcaaa acggtagctt gcgggacgga tgtaccgaaa cggtttccga ccaggaaagc
     4501 cagcaaactt agtagtaacg agggcacgat agggtggaag cccaggtact gaatattcag
     4561 cgtcgcgagt acggcataca gcacgccgcc aacgatcatc gcacttagcg cgcctttggc
     4621 gttggcgcgt tcccagtaaa gacccagcac cagcggccac aggaaaacgg cttccagccc
     4681 accgaaggcc agcaaattca gccagatgat catttctggc ggcttccagg cggcaagcag
     4741 cagcaacgcg ccgagaacta acgtaattac cgccgacatc cgcttcagac gcgtctcgtt
     4801 ttgcatttga tccggacgga tattcagata gagatcttta atgatcgtag cggaactttg
     4861 cagcaactgg gcattgatcg tcgacatgat cgcagccata ggtgcagcca ggaagattcc
     4921 ggcagcaaac ggtggcagca cttttaccat taacgtcggg atcaccagat ccggtacggt
     4981 gagatcgggg atcactgcac gacctaacgc tccggccagg tgcataccga acatcaggat
     5041 tgcgactaca atcgtaccga tgatgatccc ccggtgtact gctttgctgt ctttataaga
     5101 gatacagcgc accgcagtat gcggcaggcc aatcacgcca aagcacacca gtacccagaa
     5161 cgacgtcata aaggcaggcg acagaatatc gtcagcgcct tgtggcgtaa ccagttgcgg
     5221 atcgatggtt tgcaaggtct gtactgcgtt gcttaagccg ccagcggcat gtaccacgcc
     5281 aataagcagc acaacggtgc caatcagcat cacaagccct tgcatggtgt cgttcagcac
     5341 gctggcgcga aagccaccaa aggcggtata taacgcaatg ctgataccaa aaatcagcag
     5401 cccggtttca taaggaatac ccgccgcggt ttccagcagg cgcgcaccgc cgataaactg
     5461 cacggtcatc gcaccaacga acgcaaccag caaactcaaa ctcgccagcc acaccagaag
     5521 acgactctgg tagcgggcaa acagcatatc gttcagcgtc actgcattgt agcggcgcgc
     5581 aagaatcgca aacttcttgc cgagaatacc gagtgaaagc cagactgcag gaagctgaat
     5641 catcgccagc aatacccagc ccagcccgta tttataagca gctcctggcc cgccgataaa
     5701 cgaactggca ctgatatagg tcgcggtgag cgtcatcgcc agcacaatac cgcccataga
     5761 gcggctgccg aggaaatact cattaaggaa ggtgccggtg ctccgtttac gcatcgcata
     5821 aaccgagata ccgaacacca ccaccagata ggcgaccagc ggtagaatta cttcaagctg
     5881 catcgtcatc ctccagtggg atatcgcgat agataaattt caccatcgcc cagcacagtc
     5941 caataaacag cagcggcgtc aggatgcagg ccatctcaaa ccagcgcgga aagccggtaa
     6001 aaccgggggc aacgccaggt aagtaagcgg ctactaacca taccgccaga tacaaaaggg
     6061 tcagccccag cgcccagcgc gcctctttat gggcctgaac aaaacgagtg tccatttttt
     6121 gtccctgatg ggtgaagaaa gcgaggattg taccttatgg gggttgtcga tccccagtaa
     6181 taaaaaaggc cggaaaatcc ggccttttga cgcttttgca gtcttatttt tcctgaagac
     6241 cgagtttttt ctccagatag tggatgttag tgccaccatg ctggaagttc tcgtcattca
     6301 tgatgcggat ctgcagatca acgttggttt tgataccgtc gatgatcagc tcctgcagcg
     6361 cattcttcat gcgggcaatc gccacgtcac ggttttcacc gtagcaaatc agcttaccga
     6421 tcattgagtc atagtacggc ggtacggtgt agcccgcgta gatatgagac tcccaacgca
     6481 ctccaaaacc gccaggtgcg tggaaacggg tgattttgcc tgggcttggc aggaaggtgt
     6541 tcggatcttc ggcgttgata cgacattcca ccgcatggcc gcgaacgtga acttcttctt
     6601 gcttgatcga cagcggttga ccggcagcga tacgcagctg ttctttgatc aggtcaacgc
     6661 cggtgatcat ttcagtaacc gggtgttcta cctgaatacg ggtgttcatt tcgatgaaat
     6721 agaactcgcc gttttcgaac aggaactcga aagtacctgc accgcgatag ccgatatcaa
     6781 cacacgcttt agcgcaacgt tcgccgatgt agcgacgcag ttccggggta atgcccggtg
     6841 ctggtgcttc ttcgaccact ttctggtggc ggcgctgcat ggagcagtca cgttccgcca
     6901 gatagatagc gttgccctga ccgtcagcca gtacctgaat ctcgacgtgg cgaggatttt
     6961 ccaggtattt ctccatgtaa accatatcgt tgctgaaagc agctttcgct tccgcacggg
     7021 tcatggagat ggattgtgcc agttcagcgt cgccgcgcac tacgcgcata ccgcgaccac
     7081 cgccgccgcc ggaggctttg ataatcaccg gataaccaat gcgtttagca atggcacggt
     7141 ttttatccat atcgtcgccc agcgggccgt cagaacccgg tacgcaaggg acgcccgctt
     7201 ttttcatcgc cgcgattgcg gatactttgt cgcccatcag gcgaatggtt tctgctttcg
     7261 ggccaatgaa gataaagccg gagcgttcaa cctgctcggc aaagttggcg ttctcggaga
     7321 ggaagccgta acccggatgg attgctactg cgccggtgat ttcagcggcg cttatgattg
     7381 ccgggatgtt cagataactt tttactgacg gagcagggcc aatacagacc gtttcatctg
     7441 ccagtaatac gtgttttaga tcgcgatccg cgctggagtg cacagcgaca gtcttgatgc
     7501 ccagttcttt acaggcacga agaatacgca atgcaatctc gccgcggttg gcaataacaa
     7561 ttttatccag catgttcgcc tcgttactcg atgacgacca gcggctcgtc aaattctacc
     7621 ggttgtccac tttcgaccag aattgctttc acggtaccgg atttgtccgc ttcgatctgg
     7681 ttcatcattt tcatggcttc aacgatgcac agggtatcgc ccacgttgac tttctgaccc
     7741 acttcgatga acgcttttgc gtccgggctt ggggtgcggt agaaagtacc aaccatcggg
     7801 gagcgtacga tgtgaccact gatttccgct gctgctggcg cttccatgga aggaacggtc
     7861 gccggagcgg ctgcgttaga ttgagctggc tgctgcatca ttggtgcagc gtaagcttgt
     7921 tgcatcacag ggaaacttgc ggcaggagct gcacggctga tgcgtactga ctcttcgcct
     7981 tcggagattt ccagttcgga gatgcctgat tcttcaacca gctcgatcag ttttttaatc
     8041 ttacgaatat ccatgagtgg gttccgtact ctttgtttag tgtgattgtg acaggcgttt
     8101 caccgccgtc tgtaaagcgt atgccccgac agctgtatgc atagcgataa attccagcag
     8161 gccgggacgc cggtctattt tgccccagag tgtcaatgtt agacttgacg gacattgtgc
     8221 agcgatctgc tgctatcctc cggcaaaaaa caaaatatac cttcgttgcg ccaatgtcac
     8281 cttttccgca gcgcaaaaac tgcgtcaggg aggacgaggt cgcacattat aacgatttcg
     8341 tagcaattgg cagctaaata ctggtcttat cagggaagat aatcaacagc taacatgtaa
     8401 ataaccttca acaccgtgta atttgcaaca agccgcacaa ttcacgaaat tagcgccatc
     8461 atcgacggaa cttcttataa cgtaaggtga aaagctgtaa agccagccct gcgaagatga
     8521 ggggctgcaa tgagataatc ttcacagacc acaaataatg tagggatgcc aggatcgcga
     8581 caagatagac gacattacgc agcggttgct ggggattacc caattttcac tgcatcgtct
     8641 ggatagatgt gaatgctaaa gcacgcaaaa tcaaccgact gacaattctc agcattaaat
     8701 aggttcgagt ggttaatttg tagtcaaata aaatcagatt attcacccca tgggaccaga
     8761 aatcagtaac aggttaagcg tagtgtcgcc caggcaaagc accataaacc taacaggcga
     8821 cggaggcata tcaataatgg ctgtttagag gagtatcaat gcgcatcaat ttgtggtcag
     8881 tcgaaatcat gactttccag tttggatgat gttgtacaac ttcctgacca aggtgttcga
     8941 cgaaagctgt ccggctaacc gcttttcccg ttaaccgatt gaataatgat gtcaaaatga
     9001 gttccctctc ttattattcc ttgctaaatg gttagttaac cttcaccagc gtgcgaccct
     9061 ggatctggtt attaatgatg gcctcggcaa agttcggtgc ctctgacaga gatatctctt
     9121 ttgccgcctg ggtatagaat gattccggta aatcggcgac cagtcgctgc caggcttgtg
     9181 cgcggcgttc tggtggcgtc attactgaat ccaccccttg caaacggaca ttacgcagaa
     9241 taaatggcat gaccgtggtt ggcagagtaa aaccacccgc cagaccacag gccgccacgc
     9301 agccgccgta attcatttgc gccagcactt ttgccagcac tttgtcgcca acagtgtcaa
     9361 ttgccccagc ccagacttgt ttttccagag gacgggattc ggcaaactca tcacgaggga
     9421 gaatacgact ggcacctaaa cttttcagat attcatgggt actttcgcga ccggaaacgg
     9481 caacgacctg ataacccaac ttatgcagca gcgccacggc ggtactgccg acgccaccac
     9541 tggcacccgt cacgacaatc tccccgtcct gcgggcgaac accggcatct tccagcgcca
     9601 tcacacacag catggcggta aaaccggcag taccgataat cattgcttta cgcgcgtcca
     9661 gcccttgcgg catggcaacc agccagtcac ctttcactcg cgcctgctcc gccagcccac
     9721 cccagtggtt ttcaccaacg ccccagccag tgagtaacac ctcctgaccg gcatgaaaac
     9781 gcggatcttc gctggtgcgt acagttccgg caaaatcgat cccaggaatc atcggaaaat
     9841 tacggatgat ttttccctta ccggtaatcg ccagcgcatc tttatagttc aggctcgacc
     9901 agtgaacatc aaccgtgaca tcgccctccg gcaggcgact ttcgtcaagt gtctgtaccg
     9961 atgcgagagt tttgccgtcc tgctgttcta aaagtaacgc ctgcataagt ggtcctcatg
    10021 tgcatgatgg attggaaaat aatttctgaa gactatactc gctaatggaa aagcaaagcc
    10081 gatgaagcgc aataaattgc gagataaatc tgatttgcta gtatgcccgc ttcctcacta
    10141 tcggagttaa cacaaggatg agattaacga cgaaattttc ggcctttgtt acgctgctca
    10201 ccgggttaac aatttttgtg actttgctgg gctgttcgct aagtttctac aacgcaattc
    10261 agtataagtt tagtcatcgc gttcaggcgg tggcgacggc gattgatacc caccttgtgt
    10321 cgaatgactt cagcacatta aggccacaaa ttaccgaatt aatgatgtcg gcagatatcg
    10381 ttcgtgtaga cctgctccat ggtgataaac aggtttatac cctggccaga aatggtagtt
    10441 atcgtccggt tggctccagc gatctgtttc gcgaactgag tgttccgttg ataaagcatc
    10501 cggggatgtc gctgcgtctg gtttatcagg atccgatggg caactatttc cattctttaa
    10561 tgactaccgc gccgctcacg ggggcgattg gctttatcat tgttatgctc tttctggcgg
    10621 tacgctggtt acaacggcaa cttgccgggc aagaattgct ggaaacccgg gctactcgta
    10681 tcttaaacgg tgagcgtggc tctaatgtgt tgggaaccat ctatgaatgg ccgcccagaa
    10741 ccagcagtgc gctagatacg ctgcttcgtg aaattcagaa cgcacgcgaa caacacagcc
    10801 gtcttgatac gctgatccgc tcttatgccg cccaggacgt gaaaaccggc ctcaataacc
    10861 gactcttttt cgataatcag ttagcaacgt tactggaaga tcaggagaaa gtaggtaccc
    10921 acgggatcgt gatgatgatt cgtctgccgg atttcaatat gttgagcgat acttgggggc
    10981 acagccaggt tgaagaacag ttcttcactc tgacgaatct gctgtcgaca tttatgatgc
    11041 gctaccctgg cgcactgctg gcgcgttacc accgcagtga ttttgctgcg ctgttaccgc
    11101 accggacgtt aaaagaggca gagagcatcg ccggtcagtt aatcaaagcc gtcgatacct
    11161 tgccgaacaa taaaatgctc gatcgcgacg atatgatcca cattggtatc tgcgcctggc
    11221 gtagtggtca ggataccgag caggtaatgg aacatgcaga gtctgccacg cgtaatgcgg
    11281 gattgcaggg cggcaatagc tgggctattt acgatgactc gttgcctgaa aaaggacgcg
    11341 gtaatgttcg ctggcgtacg cttatcgagc aaatgctcag tcgcggcggc ccgcgccttt
    11401 atcaaaaacc ggcggttact cgcgaaggtc aggttcatca tcgcgaactc atgtgccgca
    11461 tcttcgatgg taatgaagag gttagctcgg cggagtatat gccgatggtc ttgcagtttg
    11521 gcttatcgga agagtatgac cgtctgcaaa tcagccgtct tattccacta ttgcgttact
    11581 ggccagagga aaatctggcg attcaggtta ccgttgagtc gctgattcgc ccgcgttttc
    11641 agcgttggct gcgcgatacg ttaatgcaat gtgaaaaatc acaacgaaaa cgcataatta
    11701 ttgaacttgc agaggccgat gtaggtcaac atatcagtcg tttacaacct gttattcgtt
    11761 tagtgaatgc tttaggggta cgggtagccg tcaaccaggc tggtttgacg ctggtaagca
    11821 ccagttggat caaagaactt aatgttgagt tactcaagct ccatccgggg ctggtcagaa
    11881 acattgagaa gcgaacggag aaccagctgc tggttcaaag cctggtggaa gcctgctccg
    11941 ggaccagcac ccaagtttac gccaccggtg tgcgttcgcg aagcgagtgg cagaccctga
    12001 ttcagcgcgg tgttacaggc gggcaagggg attttttcgc gtcctcacag ccacttgata
    12061 ctaacgtgaa aaaatattca caaagatact cggtttaacc tgccgtttaa tccgttttca
    12121 cgtagaataa cgcgcgctgc gtctcatggg agtgtgcttg tctgctcgcc agattgttgc
    12181 agcacatatg cagatgaatg accttacgcg gttgcaaaca ggcgaggaat gctgctgatg
    12241 cattaagcct ttctggactc aggcagagat ttgtaacaaa ggaaacgaac tgcactaatt
    12301 ttcaccgtag cagatgattt ttgcgccttg tcgctgctgc gtgtggttgg taaagtaagc
    12361 ggattttctt ttccgcccca gctttcagga ttatccctta gtatgttgaa aaaatttcgt
    12421 ggcatgtttt ccaatgactt gtccattgac ctgggtactg cgaataccct catttatgta
    12481 aaaggacaag gcatcgtatt gaatgagcct tccgtggtgg ccattcgtca ggatcgtgcc
    12541 ggttcaccga aaagcgtagc tgcagtaggt catgacgcga agcagatgct gggccgtacg
    12601 ccgggcaata ttgctgccat tcgcccaatg aaagacggcg ttatcgccga cttcttcgtg
    12661 actgaaaaaa tgctccagca cttcatcaaa caagtgcaca gcaacagctt tatgcgtcca
    12721 agcccgcgcg ttctggtttg tgtaccggtt ggcgcgaccc aggttgaacg ccgcgcaatt
    12781 cgtgaatccg cgcagggcgc tggtgcccgt gaagtcttcc tgattgaaga accgatggct
    12841 gccgcaattg gtgctggcct gccggtttct gaagcgaccg gttctatggt ggttgatatc
    12901 ggtggtggta ccactgaagt tgctgttatc tccttgaacg gcgtggttta ctcctcttcc
    12961 gtgcgcattg gtggtgatcg tttcgacgaa gctatcatca actatgtgcg tcgtaattac
    13021 ggttctctga tcggtgaagc caccgcagaa cgtatcaagc acgaaatcgg ttcggcttac
    13081 ccgggcgatg aagtccgtga aatcgaagtt cgtggccgta acctggcaga aggtgttcca
    13141 cgcggtttta ccctgaactc caacgaaatc ctcgaagcac tgcaggaacc gctgaccggt
    13201 attgtgagcg cggtaatggt tgcactggaa cagtgcccgc cggaactggc ttccgacatc
    13261 tccgagcgcg gcatggtgct caccggtggt ggcgcactgc tgcgtaacct tgaccgtttg
    13321 ttaatggaag aaaccggcat tccagtcgtt gttgctgaag acccgctgac ctgtgtggcg
    13381 cgcggtggcg gcaaagcgct ggaaatgatc gacatgcacg gcggcgacct gttcagcgaa
    13441 gagtaatcgg atgcaggcag gggaaatgtc tgtttaccct gcctggtctg atacgagaat
    13501 acgcataact tatgaagcca atttttagcc gtggcccgtc gctacagatt cgccttattc
    13561 tggcggtgct ggtggcgctc ggcattatta ttgccgacag ccgcctgggg acgttcagtc
    13621 aaatccgtac ttatatggat accgccgtca gtcctttcta ctttgtttcc aatgctcctc
    13681 gtgaattgct ggatggcgta tcgcagacgc tggcctcgcg tgaccaacta gaacttgaaa
    13741 accgggcgtt acgtcaggaa ctgttgctga aaaacagtga actgctgatg cttggacaat
    13801 acaaacagga gaacgcgcgt ctgcgcgagc tgctgggttc cccgctgcgt caggatgagc
    13861 agaaaatggt gactcaggtt atctccacgg ttaacgatcc ttatagcgat caagttgtta
    13921 tcgataaagg tagcgttaat ggcgtttatg aaggccagcc ggtcatcagc gacaaaggtg
    13981 ttgttggtca ggtggtggcc gtcgctaaac tgaccagtcg cgtgctgctg atttgtgatg
    14041 cgacccacgc gctgccaatc caggtgctgc gcaacgatat ccgcgtaatt gcagccggta
    14101 acggttgtac ggatgatttg cagcttgagc atctgccagc gaatacggat attcgtgttg
    14161 gtgatgtgct ggtgacctcc ggtctgggcg gtcgtttccc ggaaggctat ccggtcgcgg
    14221 ttgtctcttc cgtaaaactc gatacccagc gcgcttatac tgtgattcag gcgcgtccga
    14281 ctgcaggact gcaacgtttg cgttatctgc tgctgctgtg gggcgcggat cgtaacggcg
    14341 ctaacccgat gacgccggaa gaggtgcatc gtgttgctaa tgaacgtctg atgcagatga
    14401 tgccgcaggt attgccttcg ccagacgcga tggggccaaa gttacctgaa ccggcaacgg
    14461 ggatcgctca gccgactccg cagcaaccga cgacaggaaa tgcagctact gcgcctgctg
    14521 tgcccacaca gcctgctgct aatcgctctc cacaaagggc tacgccgccg caaagtggtg
    14581 ctcaaccgcc tgcgcgtgcg ccgggagggc aatagtggcg agctatcgta gccagggatg
    14641 ttgggtaatc tggctctctt tcctcattgc gctgttgctg caaatcatgc cctggccgga
    14701 taacctgatt gttttccggc caaactgggt gttactcatc ttgttgtatt ggatcctggc
    14761 cttgcctcat cgcgtaaatg tgggcacagg ttttgtgatg ggtgccatac tggatctgat
    14821 cagcggctcg acgcttggcg tacgcgtatt ggcgatgagc atcattgctt acctggtggc
    14881 gctgaaatac cagcttttcc gcaacctcgc attatggcag caggcgctgg tcgtcatgtt
    14941 gctttcgctg gtggtggata ttattgtttt ctgggcagag tttttagtga ttaacgtctc
    15001 tttcagaccg gaagtgttct ggagtagtgt agtcaatggg gtgctctggc cgtggatttt
    15061 cttgctgatg cgtaaagtcc gtcagcagtt tgcagtgcaa taaaggtttc tatgacttct
    15121 ctgtatttag cttccggttc tccgcgtcgt caggagttac ttgcgcaact tggcgtgacc
    15181 tttgaacgta ttgttacggg cattgaggag cagcgtcagc cgcaggagag cgcgcagcag
    15241 tatgttgtgc gtctggcgcg cgagaaagca caggcgggtg tcgcgcaaac ggcgcaggat
    15301 ctcccggtgc tgggtgcgga tactatcgtt atcctgaatg gagaagtgct ggagaaaccg
    15361 cgcgacgcag agcatgcggc gcagatgttg cgcaaattat cgggtcagac ccatcaggtg
    15421 atgacggcag tggcgttggc cgacagccag cacattctcg attgcctggt ggtcaccgat
    15481 gtgactttca gaacgttaac agacgaagac atcgcgggct atgtcgccag cggtgaaccg
    15541 ttagataaag caggtgcata cggtattcag gggctgggtg gctgttttgt caggaagata
    15601 aatggcagct atcacgccgt agtcggctta ccgctggttg aaacgtatga attattaagt
    15661 aattttaacg cactgcgtga gaaaagggat aaacatgacg gctgaattgt tagtaaacgt
    15721 aacgccttcg gaaacgcgag tggcgtatat tgatggcggt attctgcagg aaattcatat
    15781 tgaacgtgag gcgcgacgcg gaatagtagg caatatctac aagggtcgtg taagtcgtgt
    15841 acttccgggt atgcaggcgg cttttgtaga tattgggctg gataaagccg cgtttcttca
    15901 tgcatctgac atcatgccgc acaccgaatg tgtggcgggt gaagaacaaa agcaattcac
    15961 ggtgcgcgac atctcggaac tggttcgtca ggggcaagat ctgatggtgc aggtggtgaa
    16021 agatccgctt ggcactaaag gtgcgcgcct gaccaccgat atcacgctgc cttctcgcta
    16081 tctggtgttt atgccagggg cttctcacgt tggggtttcc caacgtattg aaagcgaatc
    16141 agaacgtgaa cgcctgaaaa aagtggtcgc agagtattgc gacgagcagg gcgggtttat
    16201 catccgtacc gcagcggaag gggttggcga ggctgaactg gcctccgatg ccgcttatct
    16261 gaaacgcgtc tggaccaaag ttatggagcg taaaaaacgc ccgcagaccc gttatcagct
    16321 gtacggcgaa ctggcgctgg cgcagcgtgt tctgcgtgat ttcgccgacg ccgaactgga
    16381 ccgcattcgc gttgactcac gcctgactta cgaagcattg ctggagttta cctcggagta
    16441 cattcccgag atgaccagca agctggagca ttacaccgga cgccagccga ttttcgatct
    16501 ctttgatgtc gaaaacgaaa tccagcgagc gctggaacgc aaagtagaac tgaaatccgg
    16561 tggttatctg attatcgacc agaccgaagc gatgaccacc gtggacatca ataccggagc
    16621 gtttgtcggt catcgcaatc tggacgacac cattttcaat accaatattg aagcgacgca
    16681 ggctatcgct cgccagttac ggttgcgtaa tctgggtggg attatcatta ttgatttcat
    16741 cgatatgaat aatgaagatc accgccgccg cgtgctgcac tcgctggagc aggcgttgag
    16801 caaagaccgg gtgaaaacca gcgttaatgg tttttcggcg ctggggctgg tggagatgac
    16861 gcgtaaacgc acccgcgaaa gcattgagca cgtactgtgt aacgaatgcc caacctgcca
    16921 cggtcgcgga acggtgaaaa ccgtggaaac ggtatgctat gaaatcatgc gcgagattgt
    16981 tcgtgtccac catgcttacg actccgaccg tttcctggtc tatgcatctc cggcagtagc
    17041 tgaagccttg aaaggcgaag agtcacactc gctggcggaa gtggaaattt tcgttggcaa
    17101 acaggttaaa gtacaaattg aaccgctcta taaccaggag cagtttgacg tcgtaatgat
    17161 gtaaacagat gctgggccgc catccggcaa agggtttttg agtcacattt ttagcagaca
    17221 aggagtgacg ggtgaggcga ttgccgggga ttttactgct tactggagcc gcgctcgttg
    17281 tgatcgctgc tctgctggtt agcggcctgc gtattgcttt accgcatctt gacgcctggc
    17341 gtccggaaat cctcaacaaa atagaatccg cgactggcat gccggtagaa gccagtcagc
    17401 tctcagccag ctggcagaat tttggcccga cgcttgaagc acacgacatc cgtgcagaac
    17461 taaaagatgg cggcgaattt tcggttaaac gcgttactct ggcgctggat gtctggcaga
    17521 gcctgttaca tatgcgctgg cagtttcgcg acctcacttt ctggcagctg cgctttcgca
    17581 ccaacactcc tatcaccagc ggtggtagcg atgacagtct ggaagccagt cacatcagcg
    17641 atctgtttct tcgtcaattt gaccatttcg atctccgcga cagtgaagtc agttttctga
    17701 cgccatccgg tcagcgcgcc gagctggcga tcccacaact cacctggctg aacgatccac
    17761 gtcgacaccg tgcggaaggc ctggtaagcc tctccagcct taccggacag cacggcgtga
    17821 tgcaggtgcg catggatttg cgcgatgatg aggggttgtt aagcaatggt cgcgtctggc
    17881 tacaggcgga tgacatcgac ctgaagccgt ggctcggtaa atggatgcag gacaatattg
    17941 ctctggaaac ggcacagttc tcccttgaag gctggatgac gatcgacaaa ggcgatgtaa
    18001 ccggcggtga cgtctggctg aaacagggcg gtgccagctg gttgggcgag aagcaaacgc
    18061 atacgctgtc ggtggataat ctgaccgcgc atattacgcg tgaaaatccg ggctggcagt
    18121 tctctattcc cgatacacgg atcacgatgg acggcaaacc ctggccgagc ggagcattga
    18181 cgctggcctg gataccggaa caggacgttg gcggcaaaga caataaacgc agtgacgaac
    18241 tccggattcg cgccagtaat ctggagctgg caggcctgga gggcatacgc ccgctggccg
    18301 cgaaactttc acctgcactg ggtgatgttt ggcgctccac acaaccgagc ggcaagatta
    18361 acactctggc gctggatatc ccgcttcagg cggcagacaa gacccgtttt caggcatcgt
    18421 ggagcgatct ggcctggaag caatggaaat tattaccggg tgcggaacac ttctccggga
    18481 cgctttccgg cagcgttgaa aatggtttgc ttaccgcgtc gatgaagcag gcaaagatgc
    18541 cttacgaaac ggtattccgt gcgccactgg aaatcgccga cggccaggca actataagct
    18601 ggctgaacaa tgacaaaggt ttccagctgg atgggcgtaa tattgacgtt aaagccaaag
    18661 ccgtccatgc gcgcggcggt tttcgttacc tgcaacctgc taacgatgaa ccctggctgg
    18721 gtattctggc tggcatcagt accgatgatg gttcacaagc ctggcgctat ttcccggaaa
    18781 acttgatggg taaagacctg gttgattact taagtggcgc gattcagggc ggtgaagcgg
    18841 ataacgcgac gctggtttat ggtggcaatc cgcaactctt cccctataaa cacaacgaag
    18901 gtcagtttga agtgctggtg ccgctgcgca acgcgaagtt tgccttccag ccggactggc
    18961 ctgcattaac taaccttgat attgaactgg actttattaa cgacggttta tggatgaaaa
    19021 ccgatggcgt taatctgggc ggcgtgcgcg cgagtaatct caccgcagtg atccctgact
    19081 actcgaaaga aaaactgctg attgacgctg acattaaagg tccgggtaaa gccgttggcc
    19141 cttactttga tgagacaccg ctgaaagatt ctctgggtgc gaccctgcaa gaactccagc
    19201 tcgacggcga tgtgaatgct cgcttacatc ttgatatccc gctgaacggc gaactggtaa
    19261 ccgcgaaagg tgaagtgacg ctgcgtaata acagtctgtt tatcaaacca ctcgacagca
    19321 ccctgaaaaa tttgagcggt aaattcagct ttatcaatgg cgatctgcaa agtgaaccac
    19381 tgacagcaag ctggtttaat cagccgttga acgtggattt ttccaccaaa gaaggggcaa
    19441 aagcctacca ggttgcggtg aatctcaacg gtaactggca accggcgaaa accagcgttc
    19501 tgcctgaagc ggtgaacgaa gcattgagtg gcagcgtggc gtgggatggt aaagtgggca
    19561 ttgatctgcc ttatcatgct ggcgcgacct ataacgtaga gatgaacggc gatctgaata
    19621 atgtgagtag tcacttacct tcaccgttag ccaaacctgc gggtgaacca ctagcggtaa
    19681 acgttaaggt tgatggcaat ctcaacagct ttgaattaac cggacaggct ggtgcggata
    19741 accatttcaa tagccgctgg ttgcttggtc aaaagctgac gctcgatcgt gctatttggg
    19801 cggcagacag taaaacgctc ccgccgttgc cggaacaaag tggcgttgaa ctcaatatgc
    19861 cgccgatgaa tggtgccgag tggctggccc tgttccagaa aggcgctgcg gagagtgtcg
    19921 gtggtgcagc gagtttccca caacacataa cgttacgtac gcctatgttg tcactgggaa
    19981 atcagcaatg gaataacctg agtattgttt cgcaaccgac ggcaaatggc accctggttg
    20041 aggcgcaagg gcgtgaaatc aacgccacgc tagcgatgcg taataacgcg ccgtggctgg
    20101 cgaatatcaa atatctttat tacaacccga gcgtggcgaa aactcgtggt gattcaacgc
    20161 cgtcatcacc tttcccgaca acggagcgca ttaacttccg tggctggccg gacgcacaaa
    20221 tacgatgcac agagtgctgg ttctgggggc aaaaattcgg tcgcattgac agtgatatca
    20281 ccatttctgg cgatacgtta acgctgacca atggactgat tgatactggt ttctcgcggc
    20341 ttactgccga tggtgaatgg gttaataatc cggagaatga acgtacctcg ctgaaaggaa
    20401 aactgcgcgg gcagaaaatt gatgccgccg cagaattttt tggtgtcacg acgcccatac
    20461 gccagtcgtc atttaatgtg gattacgatt tacactggcg caaagcaccc tggcaaccag
    20521 atgaggcgac gttgaatggc atcattcata ctcaactggg taaaggcgac attaccgaaa
    20581 tcaataccgg acatgccggg caattgctgc gcttattgag cgttgatgcc ctgatgcgta
    20641 agctgcgttt tgatttcaga gacacttttg gcgaagggtt ctattttgac tccattcgca
    20701 gcaccgcgtg gattaaagac ggggttatgc acaccgacga cacgctggtg gatggcctgg
    20761 aggcggatat cgccatgaaa gggtcggtaa atctggtacg tcgcgacctg aatatggaag
    20821 cggttgtcgc accagagatt tctgcgacgg tgggcgtggc tgcggctttt gcggttaacc
    20881 ccattgttgg cgcggcagta tttgcagcca gtaaagtgct ggggccgctg tggagcaaag
    20941 tctccatttt gcgctatcac atttcgggtc cgctggacga tccgcaaatc aacgaagtgt
    21001 tgcgccaacc gcgtaaagaa aaagcgcaat gatttgacga gggcgcgtaa ttgccccaat
    21061 ctcatatgat aatcgttgcc aaaggccaac gagccggaac ataactgtag gccggatagg
    21121 gcgttcacgc cgcatccggc agccgtaaaa aatcctctac tgcagtaact aacgagtagc
    21181 aaaaacgatg agtctgaacc tggtaagtga acaattgcta gcggcgaacg gcctgaaaca
    21241 tcaggacttg ttcgcgatcc tcggtcaact ggccgaacgt cgccttgatt atggcgatct
    21301 ctattttcag tcgagctatc acgaatcctg ggttttagaa gaccgcatta ttaaagatgg
    21361 ttcttacaac atcgatcagg gcgttggtgt gcgtgcaatc agtggtgaaa aaaccggatt
    21421 tgcttacgct gaccaaatca gcctgctggc gctggaacag agtgcgcaag cggcgcgcac
    21481 catcgtccgt gatagtggtg atggcaaagt acagacgctg ggcgcggtag agcatagccc
    21541 gttgtatacc tcggtagatc cgctgcaaag catgagccgt gaagagaagc tggatatcct
    21601 gcgtcgcgtc gataaggttg cccgcgaagc ggacaagcgc gtacaagaag tgactgccag
    21661 cctcagcggc gtttatgaat taattctggt tgcggccacc gacggcacgc tggcggcgga
    21721 tgttcgtccg ctggtgcgtc tttccgtgag cgttctggtc gaagaagatg gcaaacgcga
    21781 acgcggtgcc agtggcggcg gcggtcgttt tggttatgag ttcttccttg ccgatcttga
    21841 cggcgaagtc cgtgcggatg catgggcaaa agaagcagtg cgtatggcgc tggtcaatct
    21901 ttctgccgtt gctgcaccag cgggcaccat gccggtagta cttggcgcag gttggccggg
    21961 cgtgctgttg catgaagcgg taggtcacgg tctggaaggc gacttcaacc gccgcggcac
    22021 ttcagtattt agtgggcagg tcggggagct ggtggcttca gaactgtgta ccgtggttga
    22081 tgacggcacg atggtcgatc gccgtggttc ggtggcgatt gatgacgaag gtacgccagg
    22141 ccagtacaac gtgctgattg agaacggcat tctgaaaggc tacatgcagg ataaactcaa
    22201 cgcgcgtttg atggggatga cgccgactgg caacggtcgc cgtgaatcct acgcccatct
    22261 gcccatgccg cgtatgacca acacctatat gctgccgggt aaatcgaccc cgcaggaaat
    22321 tattgaatcc gttgagtacg gtatctatgc accgaacttt ggtggcggtc aggtggatat
    22381 tacatccggc aaattcgttt tctccacttc agaagcgtat ctgattgaaa acggtaaagt
    22441 aacgaagccg gtgaaaggcg cgacgttgat tggttccggt atcgaaacca tgcagcagat
    22501 ttcgatggtt ggcaacgacc tgaaactgga taacggcgtg ggtgtctgcg gtaaagaagg
    22561 gcaaagtttg ccggttggcg tgggccagcc aacgctgaaa gttgataacc tgactgttag
    22621 cggtactgcg taataatttc attattttca gaaggataat taatctttct acgtgcacga
    22681 acggtcccct cgctcctctg gggttagggt gaggggaacc cgttggcaca ggtttgtaga
    22741 acgtaacagt acaatatgaa ttacttctct ttcccgcgcc cgtgcatctc ctgaaacaat
    22801 ttaccgacct caacaaaata atccgtcagc gagttgatca cgacctgtac cttcagcggc
    22861 agcttatctt tttcggtata taacgcataa accgggcgtg gatctgactg ataacgcggc
    22921 agcaggatct ccagctcacc gcgattgatc tcgttgatca cccacatcag cggtacgtag
    22981 gcgatcccgg cacccgccgt tagccagcgc accagcgtca tcggatcatt agtcacaaat
    23041 ctcccttgcg ggatcaggcg agtcgagatc ccttccggtg caatcagttc aaattcattg
    23101 tcgggccgca cgctgtattc aagccatgaa tggctactta aatcggcggg tttttccggt
    23161 atgccgtatt gtgtgagata gcttttcgcg gcgcacacca ccattggcat cgcgccaaga
    23221 cggcgggaaa acaggctgga atcctgcaac gcgccgacgc ggatcaccac atccagaccg
    23281 tcggcaatca ggtcgggggc tggaattccg gtaaccagat tgacgctcaa acctgggtat
    23341 tctttcagca ttttggctgt cagcccggcg agaacatttt gtgccatagt tgaagaacag
    23401 ccaatgcgta gcgtcccgat gggggtgtta ttgaaggcat acagttgctc atgaacatcc
    23461 tgcacttcat gaagcatacg acggcagccc tggtagtaaa ttctaccggc ttcggtcagg
    23521 ccaatgctgc gtgtgctacg gtttaacagc tttacctgca actcatcttc cagttttgac
    23581 accgtctgac tgatggacga aacgctcatc tgtagctgtc tggcggcggc ggtaaaagag
    23641 ccaaattcaa ctactttggc aaacaccgac atgcgtttta gtcgttccat tattcactct
    23701 gacttaaaag tgatttagat cacataatat agataacagc ataacagtta cgctaatata
    23761 ttaaatatca atctacagca atgttgctct cgcccggctt tccatgccct tctctgcggc
    23821 gacagatgct gaaaataata acgcctgctc tctctataca accaaggtca acatgagtct
    23881 gtttcccgtt atcgtggtgt ttgggctgtc cttcccaccg atattttttg aattgctttt
    23941 atcactggcg attttctggc tggtgcgccg ggtacttgtg ccaacaggta tctacgactt
    24001 tgtctggcat ccggcgttgt tcaacaccgc gctctattgc tgcttgtttt atttgatatc
    24061 gcgactgttc gtttgaggtt gaagtgaaaa cactaataag aaaattctcc cgtacggcca
    24121 tcacggtcgt attagtcatt ctggccttca tcgcaatttt taatgcctgg gtctattaca
    24181 ccgaatcccc ctggacgcgt gacgcgcgct ttagcgctga cgtcgttgcg atcgcgccgg
    24241 acgtttctgg actcattacc caggtgaatg ttcatgataa ccagctggtg aaaaaaggac
    24301 agatactgtt caccatcgac cagccgcgct atcaaaaggc gcttgaggaa gcgcaagccg
    24361 atgttgctta ttatcaggta ctggcacagg agaaacgcca ggaggccgga cgtcgtaacc
    24421 gtctcggtgt gcaggcgatg tctcgcgaag agatcgacca ggctaacaac gtactacaaa
    24481 cggttctgca tcagttagcg aaagcgcagg cgacccgcga tctggcaaaa ctggatcttg
    24541 aacgcacggt gatccgcgcg ccagcagatg gctgggtgac caacctcaac gtctataccg
    24601 gtgagtttat tactcgagga tcaacggcgg ttgcgctggt gaaacagaac tccttctatg
    24661 tactggccta tatggaagaa actaagctgg aaggggtgcg tccggggtat cgtgcagaga
    24721 tcacgccgct tggcagtaac aaagtgctga aagggactgt tgatagtgtt gccgctgggg
    24781 tcaccaacgc cagcagcacg cgtgacgaca aagggatggc gaccatcgac tctaaccttg
    24841 aatgggtgcg tcttgcgcaa cgtgttccgg ttcgtattcg tctcgacaac cagcaagaga
    24901 acatctggcc tgcgggcacc actgctacag tggtggtcac tggcaaacaa gatcgcgacg
    24961 aaagccagga ttcgttcttc cgtaaaatgg cccatcgcct gcgtgagttt ggttaatcac
    25021 gatgggtatt ttctccattg ctaaccaaca tattcgcttt gcggtaaaac tggcgaccgc
    25081 cattgtactg gcgctgtttg ttggctttca cttccagctg gaaacgccac gctgggcggt
    25141 actgacagcg gcgattgttg ccgccggtcc ggcctttgct gcgggaggtg aaccgtattc
    25201 tggcgctatt cgttatcgtg gctttttgcg catcatcggc acatttattg gctgtattgc
    25261 cggactggtg atcatcattg cgatgatccg cgcaccatta ttgatgattc tggtgtgctg
    25321 tatctgggcc ggtttttgta cctggatatc ctcgctggta cgaatagaaa actcgtacgc
    25381 gtgggggctg gccggttata ccgcgctgat cattgtgatc accattcagc cggaaccatt
    25441 gcttacgccg cagtttgccg tcgaacgttg tagcgagatc gttatcggta ttgtgtgtgc
    25501 gattatggcg gatttgctct tttctccgcg atcgatcaaa caagaagtgg atcgagagct
    25561 ggaaagtttg ctggtcgcgc aatatcaatt aatgcaactc tgtatcaagc atggcgatgg
    25621 tgaagttgtc gataaagcct ggggcgacct ggtgcgacgc accacggcgc tacaaggcat
    25681 gcgcagcaac ctgaatatgg aatcttcccg ctgggcgcgg gccaatcgac gtttaaaagc
    25741 gatcaatacg ctatcgctga cgctgattac ccaatcctgc gaaacttatc ttattcagaa
    25801 tacgcgcccg gaattgatca ctgatacttt ccgcgaattt tttgacacgc cggtagaaac
    25861 cgcgcaggac gtccacaagc agctcaaacg cctgcggaga gttatcgcct ggaccgggga
    25921 acgggaaacg cctgtcacca tttatagctg ggtcgcggcg gcaacgcgtt atcagcttct
    25981 caagcgcggc gttatcagta acacaaaaat caacgccacc gaagaagaga tcctgcaagg
    26041 cgaaccggaa gtcaaagtag agtcagccga acgtcatcat gcgatggtta acttctggcg
    26101 aaccacactt tcctgcattc tgggaacgct tttctggctg tggacgggct ggacttccgg
    26161 cagtggtgca atggtgatga ttgcggtcgt gacgtcactg gcaatgcgtt tgccgaatcc
    26221 acgcatggtg gcgatcgact ttatctacgg gacgctggcc gcgctgccgt tagggctgct
    26281 ctactttttg gtgattatcc ctaataccca acagagcatg ttgctgctgt gtattagcct
    26341 ggcagtgctg ggattcttcc tcggaataga agtacagaaa cggcgactgg gctcgatggg
    26401 ggcgctggcc agcaccataa atattatcgt gctggataac ccgatgactt tccatttcag
    26461 tcagtttctc gacagcgcat tagggcaaat cgtcggctgt gtgctcgcgt tcaccgttat
    26521 tttgctggtg cgggataaat cgcgcgacag gactggacgt gtactgctta atcagtttgt
    26581 ttctgccgct gtttccgcga tgactaccaa tgtggcacgt cgtaaagaga accacctccc
    26641 ggcactttat cagcagctgt ttttgctgat gaataagttc ccaggggatt tgccgaaatt
    26701 tcgcctggcg ctgacgatga ttatcgcgca ccagcgcctg cgtgatgcgc cgatcccggt
    26761 taacgaggat ttatcggcgt ttcaccgaca aatgcgccgc acagcagacc atgtgatatc
    26821 tgcccgtagc gatgataaac gtcgtcggta ctttggtcag ttgctggaag aactggaaat
    26881 ctaccaggaa aagctacgca tctggcaagc gccaccgcag gtgacggaac cggttcatcg
    26941 gctggcgggg atgctccata agtatcaaca tgcgttgacc gatagttaag tcaaaaccga
    27001 cgccaaaagc gtcggttttt tcatggctat acttagcgat gaacggcaga actcgccgcg
    27061 aaacgtgacg gtggcaacag atgaatattt atacctttga ttttgatgag attgagagtc
    27121 aggaggattt ttatcgcgac ttcagccaag cctttggtct ggcgaaacat aaggtacgcg
    27181 atctcgactc actatgggat gtgttaatga acgatgtcct gccgctacca cttgagattg
    27241 aatttgttca tctgggagag aaaacgcgtc gccgttttgg cgcgttaata ttgctgtttg
    27301 atgaggcaga ggaagagctg gaagggcatt tgcgttttaa tgttcgtcat tagcgcaaaa
    27361 aaaagccccc gaaccggggg caatatcgtc ggacaagacg atgagggttt atttgtacag
    27421 ttcagccgta gcgtgccagg tgtcaccgct acgagcttca gtaatctggt aggccgttgc
    27481 gcctttctct tccgcttttt tgttcagcat ttcacgtata tccattggcg aagacgccac
    27541 accacttacg gatacggtcc cgattgcttc acggttttgt gcttgtgcag catcaatgga
    27601 gtcggcagcg aatgcaccga aagagagaac agaaagtacg cttaatgcag caacagtggt
    27661 tttgattttc atgattttta cctcgacata atctttttgc tgggtctttg tttcgtgacc
    27721 cttatcacaa aatcaagtat acactaatta ttgaactaat taataccacg ctaactgttt
    27781 ctgttgatac taa
//
LOCUS       DAZFQD010000059        27744 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.059, whole genome shotgun sequence.
ACCESSION   DAZFQD010000059 DAZFQD010000000
VERSION     DAZFQD010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27744
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.059"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            195..446
                     /gene="chpS"
                     /locus_tag="ACHKTW_002567"
     CDS             195..446
                     /gene="chpS"
                     /locus_tag="ACHKTW_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin ChpS"
                     /protein_id="HFO6463570.1"
                     /translation="MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEAQVSNNQLILT
                     PISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGDEIW"
     gene            440..790
                     /gene="chpB"
                     /locus_tag="ACHKTW_002568"
     CDS             440..790
                     /gene="chpB"
                     /locus_tag="ACHKTW_002568"
                     /EC_number="4.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease toxin ChpB"
                     /protein_id="HFO6463571.1"
                     /translation="MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLG
                     MTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLVNQVRMMDLRARLAKRIGLAADE
                     VVEEALLRLQAVVE"
     gene            complement(870..1400)
                     /gene="ppa"
                     /locus_tag="ACHKTW_002569"
     CDS             complement(870..1400)
                     /gene="ppa"
                     /locus_tag="ACHKTW_002569"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HFO6463572.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGE
                     DAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            1710..2666
                     /gene="ytfQ"
                     /locus_tag="ACHKTW_002570"
     CDS             1710..2666
                     /gene="ytfQ"
                     /locus_tag="ACHKTW_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
                     /protein_id="HFO6463573.1"
                     /translation="MWKRLLVVSAVSAAMSSMALAAPLTVGFSQVGSESGWRAAETNV
                     AKSEAEKRGITLKIADGQQKQENQIKAVRSFVAQGVDAIFIAPVVATGWEPVLKEAKD
                     AEIPVFLLDRSIDVKDKSLYMTTVTADNILEGKLIGDWLVKEVNGKPCNVVELQGTVG
                     ASVAIDRKKGFAEAIKNAPNIKIIRSQSGDFTRSKGKEVMESFIKAENNGKNICMVYA
                     HNDDMVIGAIQAIKEAGLKPGKDILTGSIDGVPDIYKAMIDGEANASVELTPNMAGPA
                     FDALEKYKKDGTMPEKLTLTKSTLYLPDTAKEELEKKKNMGY"
     gene            2806..4308
                     /gene="ytfR"
                     /locus_tag="ACHKTW_002571"
     CDS             2806..4308
                     /gene="ytfR"
                     /locus_tag="ACHKTW_002571"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfR"
                     /protein_id="HFO6463574.1"
                     /translation="MTTNQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQAISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGFLRRKEMEKRATELMTSYGFSLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEVELLFGLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHALQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKQQNLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDEPTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAELSVPAIMNAIAA"
     gene            4322..5344
                     /gene="ytfT"
                     /locus_tag="ACHKTW_002572"
     CDS             4322..5344
                     /gene="ytfT"
                     /locus_tag="ACHKTW_002572"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfT"
                     /protein_id="HFO6463575.1"
                     /translation="MPQSLPDTTPPKRRFRWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GVSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITSGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEAVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSRDKT"
     gene            5331..6326
                     /gene="yjfF"
                     /locus_tag="ACHKTW_002573"
     CDS             5331..6326
                     /gene="yjfF"
                     /locus_tag="ACHKTW_002573"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, permease
                     protein YjfF"
                     /protein_id="HFO6463576.1"
                     /translation="MIKRNLPLMITIGVFVLGYFYCLTQFPGFASTRVICNILTDNAF
                     LGIIAVGMTFVILSGGIDLSVGSVIAFTGVFLAKVIGDFGLSPLLAFPLVLVMGCAFG
                     AFMGLLIDALKIPAFIITLAGMFFLRGVSYLVSEESIPINHPIYDTLSSLAWKILGGG
                     RLSAMGLLMLAVVVIGIFLAHRTRFGNQVYAIGGNATSANLMGISTRSTTIRIYMLST
                     GLATLAGIVFSIYTQAGYALAGVGVELDAIASVVIGGTLLSGGVGTVLGTLFGVAIQG
                     LIQTYINFDGTLSSWWTKIAIGILLFIFIALQRGLTVLWENRQSSPVTRVNIAQQ"
     gene            complement(6359..7357)
                     /gene="fbp"
                     /locus_tag="ACHKTW_002574"
     CDS             complement(6359..7357)
                     /gene="fbp"
                     /locus_tag="ACHKTW_002574"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HFO6463577.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            7533..8906
                     /gene="mpl"
                     /locus_tag="ACHKTW_002575"
     CDS             7533..8906
                     /gene="mpl"
                     /locus_tag="ACHKTW_002575"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HFO6463578.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLDPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPTHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            complement(9062..9613)
                     /gene="yjgA"
                     /locus_tag="ACHKTW_002576"
     CDS             complement(9062..9613)
                     /gene="yjgA"
                     /locus_tag="ACHKTW_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HFO6463579.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRAAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            9707..11059
                     /gene="pmbA"
                     /locus_tag="ACHKTW_002577"
     CDS             9707..11059
                     /gene="pmbA"
                     /locus_tag="ACHKTW_002577"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HFO6463580.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGVADKELLAFDAPDLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            11242..11628
                     /gene="cybC"
                     /locus_tag="ACHKTW_002578"
     CDS             11242..11628
                     /gene="cybC"
                     /locus_tag="ACHKTW_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HFO6463581.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLEDNMETLNDNLKVIEKADN
                     AAQVKDALTKMRAAALDAQKATPPKLEDKSPDSPEMKDFRHGFDILVGQIDDALKLAN
                     EGKVKEAQAAAEQLKTTRNAYHQKYR"
     gene            complement(11673..12137)
                     /gene="nrdG"
                     /locus_tag="ACHKTW_002579"
     CDS             complement(11673..12137)
                     /gene="nrdG"
                     /locus_tag="ACHKTW_002579"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HFO6463582.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QTFTKAMEDQIINDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVKRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(12295..14433)
                     /gene="nrdD"
                     /locus_tag="ACHKTW_002580"
     CDS             complement(12295..14433)
                     /gene="nrdD"
                     /locus_tag="ACHKTW_002580"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HFO6463583.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVSEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVKVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFGGEHVY
                     DNEQLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            complement(14827..16482)
                     /gene="treC"
                     /locus_tag="ACHKTW_002581"
     CDS             complement(14827..16482)
                     /gene="treC"
                     /locus_tag="ACHKTW_002581"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HFO6463584.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYISPQVDNGYDVANYTAIDPIYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DESSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMNLRPFEAVWWLQK"
     gene            complement(16532..17953)
                     /gene="treB"
                     /locus_tag="ACHKTW_002582"
     CDS             complement(16532..17953)
                     /gene="treB"
                     /locus_tag="ACHKTW_002582"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HFO6463585.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMVIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            complement(18072..19019)
                     /gene="treR"
                     /locus_tag="ACHKTW_002583"
     CDS             complement(18072..19019)
                     /gene="treR"
                     /locus_tag="ACHKTW_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor TreR"
                     /protein_id="HFO6463586.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMESQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            19204..19257
                     /gene="mgtL"
                     /locus_tag="ACHKTW_002584"
     CDS             19204..19257
                     /gene="mgtL"
                     /locus_tag="ACHKTW_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HFO6463587.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            19398..22094
                     /gene="mgtA"
                     /locus_tag="ACHKTW_002585"
     CDS             19398..22094
                     /gene="mgtA"
                     /locus_tag="ACHKTW_002585"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HFO6463588.1"
                     /translation="MFKEIFTRLIRHLPSRLVHRDPLPGAQQTVNTAVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGENKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDIMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKMTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMIFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(22300..22686)
                     /gene="ridA"
                     /locus_tag="ACHKTW_002586"
     CDS             complement(22300..22686)
                     /gene="ridA"
                     /locus_tag="ACHKTW_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HFO6463589.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(22759..23220)
                     /gene="pyrI"
                     /locus_tag="ACHKTW_002587"
     CDS             complement(22759..23220)
                     /gene="pyrI"
                     /locus_tag="ACHKTW_002587"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009347 - aspartate carbamoyltransferase
                     complex [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HFO6463590.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEDQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            complement(23233..24168)
                     /gene="pyrB"
                     /locus_tag="ACHKTW_002588"
     CDS             complement(23233..24168)
                     /gene="pyrB"
                     /locus_tag="ACHKTW_002588"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HFO6463591.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VIAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            complement(24172..24306)
                     /gene="pyrL"
                     /locus_tag="ACHKTW_002589"
     CDS             complement(24172..24306)
                     /gene="pyrL"
                     /locus_tag="ACHKTW_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HFO6463592.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCPGVRR
                     "
     gene            complement(24587..24982)
                     /gene="yjgH"
                     /locus_tag="ACHKTW_002590"
     CDS             complement(24587..24982)
                     /gene="yjgH"
                     /locus_tag="ACHKTW_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFO6463593.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPENQFEDIMTVKNEIFSAP
                     PYPNWTAVGVTWLAGFDFEIKVIARIPEQ"
     gene            complement(25113..25826)
                     /gene="bdcA"
                     /locus_tag="ACHKTW_002591"
     CDS             complement(25113..25826)
                     /gene="bdcA"
                     /locus_tag="ACHKTW_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO6463594.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     ERLAQETGATAVFTDSADRDAVIDVVRKSGALDIHVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     ARDFGPRGITINVVQPGPIDTDANPANGPMRDMLHGFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            25897..26490
                     /gene="bdcR"
                     /locus_tag="ACHKTW_002592"
     CDS             25897..26490
                     /gene="bdcR"
                     /locus_tag="ACHKTW_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709951.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFO6463595.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVAEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLADILLDDRPVGECLVEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAVQYYHAAETTIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSIEQLCATAALAGEAIKTILKE"
     gene            26635..27087
                     /gene="tabA"
                     /locus_tag="ACHKTW_002593"
     CDS             26635..27087
                     /gene="tabA"
                     /locus_tag="ACHKTW_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HFO6463596.1"
                     /translation="MIIGNIHNLQPWLPQELRQAIEHIKAHVTVETPKGKHDIEGNRL
                     FYLISEDMTEPYEARRAEYHARYLDIQIVLKGQEGMTFSTQPAGTPDTDWLADKDIAF
                     LPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKMLMA"
     gene            27209..>27744
                     /locus_tag="ACHKTW_002594"
     CDS             27209..>27744
                     /locus_tag="ACHKTW_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO6463597.1"
                     /translation="MSKISGWNFSQNITSADNCKQKNEDLDTWYVGMNDFARIAGGQN
                     SRSNILSPRAFLEFLAKIFTLGYVDFSKRSNEAGRNMMAHNKSSSYIKNNDGSEIMKF
                     VMNNPEGERADLSKVEIEITLASAFNNGIREGHTVIIFTQPDGSTNRYEGKSFERKDE
                     SSLHLITNKILACYQREA"
BASE COUNT         6569 a         6940 c         7416 g         6819 t
ORIGIN      
        1 cttcgggtgg cgttgttttt tgcgagacga ctcgcattct gttttgtaat tccctcaccc
       61 tttgcttttc tctccgagcc gctttccata tctgttaacg cataaaaaac tctgctggcg
      121 ttcacaaatg cgcaggggta aaacgtttcc tgtagcaccg tgagttatac tttgtataac
      181 ttaaggaggt gcagatgcgt attaccataa aaagatgggg gaacagtgca ggtatggtca
      241 ttcccaatat cgtaatgaaa gaacttaact tacagccggg gcagagcgtg gaagcgcagg
      301 tgagcaacaa ccaactgatt ctgacaccca tctccaggcg ctactcgctt gatgaactgc
      361 tggcacagtg tgacatgaac gccgcggaac ttagcgagca ggatgtctgg ggtaaatcca
      421 cccctgcggg tgacgaaata tggtaaagaa aagtgaattt gaacggggag acattgtgct
      481 ggttggcttt gatccagcaa gcggccatga acagcaaggt gctggtcgac ctgcgcttgt
      541 gctctccgtt caagccttta atcaactggg aatgacgctg gtggccccca ttacgcaggg
      601 cggaaatttt gcccgttatg ccggatttag cgttccttta cattgcgaag aaggcgatgt
      661 gcacggcgtg gtgctggtga atcaggtgcg gatgatggat ctacgcgccc ggctggcaaa
      721 gcgtattggt ctggctgcgg atgaggtggt ggaagaggcg ttattacgct tgcaggcggt
      781 ggtggaataa ggtgtgttta tttatcgctg gcataaaaaa acccttacta accgaagctc
      841 ggcgttcagg gttattgcgc tagaagaact tatttattct ttgcgcgctc gaaggaagca
      901 acgatttcag ctttagcggc ttctgcgttt tcccaacctt caactttcac ccacttgcct
      961 ttttcgaggt ctttgtagtg ctcgaagaag tgagcgatct gcgctttcag cagctctggc
     1021 agatcgttaa cgtctttaat gtgatcgtat tctttgctca gcttgctgtg cggaaccgca
     1081 accagtttcg catcttcacc ggcttcgtcg gtcattttca gaacgccaac cggacggcaa
     1141 cggatcacag aacccggctg cagcgggtac ggagtcggga ccagtacgtc aaccgggtca
     1201 ccgtccagag acagggtgtg gttgatgtaa ccgtagttgc acggatagaa catcgcggtg
     1261 gacatgaagc ggtcaacgaa cagtgcgccg ctctctttgt cgatttcgta tttgatcgga
     1321 tctgcgttag ccgggatctc aataacaacg tagatgtctt ccggcagatc tttacccgca
     1381 gggacgttga gtaagctcat gtctgtttcc tttaaaaata tgtggcaaac aagtgccgag
     1441 tattatagcc aactcgcgcc gaatgtcttt gcttgttttc gtcttcattt tcctttttcg
     1501 ccagttttaa gacggtatac agagcaggaa aatccataac cgtagccgca tttttcatag
     1561 tgagatgaaa gcgattacaa acttgtgatt aacgttttat ttactttttt gaagtgtgat
     1621 gtaacgcaat ccgttacata acgaattgtc tatagttttt tcgcgaacat cttttcacca
     1681 ataataatta ccccgacgag gacaacccta tgtggaaacg cttacttgta gtctctgcag
     1741 tctcggcagc catgtcgtct atggcgttgg ccgctccatt aaccgtagga ttttcgcagg
     1801 tcggatcgga atccggctgg cgcgctgcag aaaccaatgt agcgaaaagt gaggccgaaa
     1861 aacgcggaat tacgttgaaa attgctgatg gtcagcaaaa gcaggaaaac cagattaaag
     1921 cggtacgttc cttcgttgca caaggggtgg atgcgatctt tatcgctccg gtggtcgcga
     1981 caggttggga accggtatta aaagaggcga aagatgccga aatcccggtc ttcttgctcg
     2041 accgttccat cgatgtgaaa gacaaatctc tctatatgac caccgttact gccgacaaca
     2101 tcctcgaagg taagttgatc ggtgactggc tggtaaaaga agtgaatggc aaaccatgca
     2161 acgtggtgga gctgcagggc accgttgggg ccagcgtcgc cattgaccgt aagaaaggct
     2221 ttgccgaagc cattaagaat gcgccaaata tcaaaatcat ccgctcgcag tcaggtgact
     2281 tcacccgcag taaaggcaaa gaagttatgg agagctttat caaagcggaa aacaacggca
     2341 aaaacatctg catggtttac gcccataacg atgacatggt gattggcgca attcaggcaa
     2401 ttaaagaagc gggcctgaaa ccgggcaaag atatcctcac gggttccatt gacggtgtac
     2461 ccgatatcta caaagcgatg attgatggcg aagcgaacgc cagtgttgaa ctgacgccga
     2521 atatggcagg tcccgccttc gacgcgctgg agaaatacaa aaaagacggc accatgcctg
     2581 aaaagctgac gctgaccaaa tccacccttt atctgcctga taccgcaaaa gaagagttag
     2641 agaagaagaa aaatatgggg tattgagggt tgctatgcct gatgccgatt cgtaggccgg
     2701 ataaggcgct cgcgccgcat ccggcggtgg tgcgctgaag cctgatgcga cgcttaccgc
     2761 gtcttatcat gcctactggg agcacgcttt acaccggggg aaaccatgac gactaaccaa
     2821 caccaggaga tcctccgcac cgaaggatta agtaaatttt tccccggcgt caaagcgtta
     2881 gacaacgttg atttcagcct gcgccgtggc gaaatcatgg cgctgctcgg tgaaaacggg
     2941 gcgggaaaat cgacgctaat caaagcatta accggtgtat accacgccga tcgcggcacc
     3001 atctggctgg aaggccaggc tatctcaccg aaaaataccg cccatgcaca acaactcggc
     3061 atcggaaccg tttatcagga agtcaacctg ctacccaata tgtcggtcgc tgataatcta
     3121 ttcataggtc gcgaacccaa acgtttcggc tttctacgcc gcaaagagat ggaaaagcgt
     3181 gccaccgaac tgatgacatc ttacggtttc tccctcgacg tgcgtgaacc gcttaaccgt
     3241 ttttcggtgg cgatgcagca aatcgtcgcc atttgccggg ctatcgatct ctccgccaaa
     3301 gtgctgatcc tcgatgaacc caccgccagc ctcgacaccc aggaagtaga gttactgttt
     3361 ggcctgatgc gtcagttgcg cgatcgcggc gtcagcctga tctttgtcac tcactttctc
     3421 gatcaggtct atcaggtcag cgatcggatc accgtcttac gcaacggcag tttcgtaggc
     3481 tgtcgggaaa cgcgcgagct accgcagatc gaactggtaa aaatgatgct ggggcgcgag
     3541 ctggataccc acgcgctaca gcgtgccggg cgaacattgt tgagcgacaa acccgttgcc
     3601 gcgttcaaaa attacggcaa aaaaggaacg atcgcaccgt ttgatctcga agtacgcccc
     3661 ggcgagatcg tcggcctggc aggcttgctg ggatccggac gtaccgaaac cgccgaagtg
     3721 atcttcggta tcaaacctgc tgacagcggc acggcgttga tcaaaggcaa acagcaaaac
     3781 ctgcgatcgc cacatcaggc ttcggtactg ggcatcggct tctgcccgga agacaggaaa
     3841 accgatggca tcatcgccgc cgcctcggta cgggaaaata tcatcctcgc tctccaggcc
     3901 cagcgcggct ggctacgacc catttcccgc aaagagcagc aagagattgc cgaacgcttt
     3961 atccgccagc ttggcattcg cacaccttca actgaacaac cgattgaatt tctctctggc
     4021 ggcaatcagc aaaaagtgtt gctttcacgt tggctactga cccgaccgca atttctgatc
     4081 ctcgacgagc caacccgcgg cattgatgtt ggtgcccacg ccgagatcat ccgcctgatt
     4141 gaaacgctgt gtgctgacgg tctggcgctg ttggtgatct cctccgaact ggaagagctg
     4201 gtgggctatg ccgatcgggt gattatcatg cgtgatcgca aacaggtggc ggagatcccg
     4261 ctggcagagc tttccgttcc ggcgatcatg aacgccattg cggcgtaagg agaacagtgt
     4321 gatgcctcaa tctctcccgg acactacgcc gccgaaaagg cgctttcgct ggccaacggg
     4381 aatgccgcag ctggcggcgc tattgctggt gctgctggtc gatagcctgg tagctccgca
     4441 tttctggcag gtggtgcttc aggatgggcg tttgttcggt agccccatag acattcttaa
     4501 ccgtgcagca cccgttgcgt tactggcgat tggtatgacg ctggtgatcg ccaccggtgg
     4561 gattgatctc tccgtagggg cggtgatggc tatcgccgga gccacaacgg ctgcgatgac
     4621 ggtcgcggga gtcagcctgc cgattgtttt gttaagcgcc ctgggcacag gcatcctggc
     4681 gggattgtgg aacggcatac tggtagcgat cctcaaaatt cagccatttg tcgccaccct
     4741 gatcctgatg gtcgccgggc gcggcgtggc gcaactgatc acctccggac agatcgtcac
     4801 gtttaactcg ccggatctct catggtttgg cagtggatcg ctgttgttcc tgccaacgcc
     4861 agtcattatt gcggtactga cgcttctcct gttctggctg ttgacccgca aaacggcact
     4921 ggggatgttt atcgaagccg ttggtatcaa cattcgggcg gcaaaaaatg ccggggtaaa
     4981 cacgcgaatc atcgtcatgc tcacctacgt gttgagcggg ctatgtgcgg cgattgcggg
     5041 cattatcgtg gcggcggata ttcgcggtgc cgatgccaac aacgccggat tatggctgga
     5101 gctggacgcc attctcgcgg tggtgattgg cggcggatcg ctgatgggcg ggcgctttaa
     5161 cctgctgctc tcggtagtgg gggcgctgat tattcagggg atgaacaccg gaattttgct
     5221 ttcgggcttt ccaccggaga tgaaccaggt ggtgaaagcg gtggtggtgc tttgcgtgct
     5281 gattgtccag tcgcaacgct ttatcagtct gattaaagga gtacgtagcc gtgataaaac
     5341 gtaatttgcc actgatgatc accatcggcg tctttgtgct gggctatttc tactgcctga
     5401 cgcagtttcc cggttttgct tccacaagag tgatctgcaa tatcctgacc gataacgctt
     5461 ttctggggat tattgccgtt ggtatgacct ttgtgatcct ctctggcggt atcgatctct
     5521 ccgtcggttc ggtgatcgct tttaccggcg tgtttctggc aaaagtgatt ggcgacttcg
     5581 gcctctcgcc gttgctggca tttccgctgg tgctggtaat gggctgtgcc tttggcgcat
     5641 ttatggggct tctgatcgac gccctgaaga tcccggcatt tatcattacg cttgcgggga
     5701 tgttcttttt gcgcggcgtc agctatctcg tttcggaaga gtcgattccg ataaaccatc
     5761 ccatttatga cacgctctca agccttgcgt ggaaaatcct tggcggcggt cgcttaagtg
     5821 cgatggggct gctgatgctg gcggtggtgg ttatcggcat attcctcgcg catcgtaccc
     5881 gttttggtaa tcaggtatac gccattggcg gcaacgcaac gtcggcaaac ctgatgggga
     5941 tttctacccg cagcaccact attcgcattt atatgctctc caccggactg gcaacgctgg
     6001 cggggattgt cttctcgatt tatacccagg ccggatatgc gctggcgggc gtaggtgtgg
     6061 aactggacgc tatcgcctca gtggtgattg gcggtacgct tttgagcggt ggcgttggaa
     6121 cggtattagg gacgcttttt ggcgtggcga ttcagggact gattcagact tacataaact
     6181 ttgatggcac gctgagttcc tggtggacga aaatcgccat cggtattttg ttgtttattt
     6241 ttatagcatt acagcgtgga ttaacggtgc tgtgggagaa tcgtcagagt tcgccagtga
     6301 caagagtcaa cattgcgcag caataaaaac gcctctccgt gtggagaggc gcaggagatt
     6361 acgcgtccgg gaactcacgg ataaagcgtt cgacatcttc aaccatatgg tcgttgccga
     6421 caaagaacga acggcgctgg tgcagggttt ccgggataat atccagaata cgctctttgc
     6481 catcgctcgc tttaccgccc gcttgttccg ccaggaatgc catcgggttg cactcataca
     6541 gtaaacgcag tttaccgtcc gggtggctgg cggtgcttgg gtagagataa ataccgcctt
     6601 tcagcaggtt acggtggaaa tccgcaacca gtgaaccgat ataacgtgag gtatacgggc
     6661 ggttggtgga tttatcttct tcctggcaga atttaatgta cttcttcacc ccgttcggaa
     6721 acttaatgta gtttccttcg ttgatggagt aggttttgcc tttctccggg aagcgcatcc
     6781 gttcctggca caggcagaaa acgcccagcg aaggatcgta agtaaaggcg tgaacaccgc
     6841 atccggtggt gtaaaccagc atagtagagg agccgtatac cacgtaacct gccgcaacct
     6901 gtttgttacc aggctggagg aaatcttctt ccgttaccgg cgtgccaaca ggcgtaacgc
     6961 ggcggtagat ggagaaaatg gtaccgacag agacgttaac atcgatgttg gacgagccat
     7021 ccagggggtc catcagcacc acgtattttg cgtgttcaca gccttcaaag acgacaatct
     7081 catcttcttc ttcagaggca atgcccgcaa cgatatcgcg tgctttcagt gcggctttca
     7141 gtttttcatt agcgaacaag tcgagtttct gctgaacctc gccctgcacg ttctcagcac
     7201 cgctggcacc caggatatca accagtcctg ctttgttgat atcgcgatgg ataatcttgg
     7261 cgcccagttt tattgccgac agcaaagcag tgagctcacc ggtagcatga gaaaactcgt
     7321 gctgcttttc gacaataaat tcacctaacg ttttcataaa actttccctg caatgtttat
     7381 ggagtaaagc gaccgcaaca atcttaacaa ataatctcaa tgttgcgctc aggtgaatcg
     7441 cgccagcaaa ttgcggatta tcctgaaatg cgtttctcac ttgcccgaca tatgcgtaaa
     7501 atgagcggca gattaaaaaa ggatagtgac gtatgcgcat tcatatttta ggaatttgtg
     7561 gcacgtttat gggcggtctg gcgatgctgg cgcgccagtt aggccatgaa gtaacgggtt
     7621 cggacgccaa tgtgtatccg ccgatgagca ccttacttga gaagcaaggc attgaactga
     7681 ttcagggtta cgatgccagc cagctcgatc cgcagccgga tctggtgatt attggcaacg
     7741 ccatgacccg tggaaatccg tgtgtggaag cggtactgga aaaaaacatc ccttatatgt
     7801 caggtccaca gtggctgcac gattttgtgc tgcgcgaccg ctgggtgctg gccgttgccg
     7861 gtacacacgg caaaaccacc accgcgggaa tggcgacctg gattctggaa cagtgtggtt
     7921 acaaaccggg ctttgtaatc ggcggtgtgc cggggaactt tgaggtttcg gcgcgtctgg
     7981 gcgaaagcga cttctttgtt atcgaagcgg atgagtatga ctgcgccttc ttcgacaaac
     8041 gctctaaatt tgttcattac tgcccgcgta cgctgatcct caacaacctt gagttcgatc
     8101 acgccgatat ctttgacgac ctgaaagcga tccagaaaca gttccaccat ctggtgcgta
     8161 tcgtaccggg gcagggccgt attatctggc cggaaaatga catcaacctg aaacagacca
     8221 tggcgatggg ctgctggagc gagcaggagc tggtgggtga gcagggtcac tggcaggcga
     8281 aaaagctgac caccgatgct tccgaatggg aagtcttgct ggatggcgaa aaagtgggcg
     8341 aagtgaaatg gtcgctggta ggcgaacata atatgcacaa tggcctgatg gcgattgcgg
     8401 cggctcgcca tgttggtgta gcgccggcag atgccgctaa tgcgctgggt tcgtttatta
     8461 acgctcgtcg ccgtctggaa ctgcgtggtg aagcgaatgg cgtcacggta tatgatgatt
     8521 tcgcccatca cccaacggcg attctggcaa cgctggcggc gctgcgtggc aaagttggcg
     8581 gtacggcgcg cattattgct gtgctggaac cgcgctcgaa taccatgaaa atggggatct
     8641 gcaaagacga tctggcacct tcattaggcc gtgccgatga agtcttcctg ctgcaaccga
     8701 cgcatattcc gtggcaggtg gcagaagtgg cagaagcctg cgttcagcct gcacactgga
     8761 gtggcgatgt ggatacgctg gcagatatgg tggtgaaaac cgctcagcct ggcgaccata
     8821 ttctggtgat gagcaacggc ggttttggtg gcatccatca gaaactgctg gatggtctgg
     8881 cgaagaaagc ggaagccgcg cagtaattcg gccttagcct gatatagtgt gcgtaggccg
     8941 gataaggcgt tcacgccgca tccggcattt gagcatagtg cctgatgcga cgcttgatgc
     9001 gtcttatcag gcctacaggt gcaccgcatc cggcaaacca tactcacatc aacaacgaaa
     9061 attacccttc attctccgcc agttcgcgca gatactggaa aatctggcgt gcggatttcg
     9121 gcggcttatt cccttctttc tctttcttcg cgttgcggat cagagtacgc agctgctgac
     9181 gatcggcatc gggccacaga tttaatacct cagcgatggc gtcatcacct tgatcgatca
     9241 gacgatcgcg caggttttcc agtttatgga acagcaccac ctgctggttg tgacggtttt
     9301 tcagcttatc cagcgcctga cggatcggtt ctacatcgcg ctggcgcagc atcttaccga
     9361 tgagctgcaa ctggcggcgg cgaccttcca tcttaatacg ctgggcgagc tcaatagccg
     9421 cgcgcagatc cgcatctaac gggatcttat ccagcgcgtt tttccccaga tcaacaattt
     9481 ccgcgccaag gcgttttagc tcctcggcat cacgtttaat ttcactttta ctgacccaga
     9541 taatttcatc gtcttcgtct tcgatgtcat caccgggaac gtcgtcgagc cagtcttcgg
     9601 gctgcttagt catctcaggc tccttaaaaa aagaggctaa tgttaccagt taagatgcgc
     9661 actgaaaaac ggttctctgt tagacttcag agaaactctc tacattatgg cacttgcaat
     9721 gaaagtaatc tctcaagttg aagcgcagcg caagattctg gaagaagcag tttccactgc
     9781 gctggagttg gcctcaggca aatcggacgg tgcggaagtt gccgtcagca agaccaccgg
     9841 cattagcgta agcacgcgtt atggtgaagt ggagaatgtt gaattcaata gcgatggcgc
     9901 gctggggatc actgtttatc accagaaccg caaaggtagc gcatcatcca ccgatttaag
     9961 cccgcaggcc attgcccgta ctgtacaggc ggcgctggat attgcccgtt atacctcgcc
    10021 agatccctgt gccggcgtgg cagacaaaga gctgctggcc tttgacgcac cagatctcga
    10081 cttgttccac cctgcggaag tttccccgga tgaagccatt gaactggcgg cccgcgcaga
    10141 acaggcggca ttgcaggcgg acaaacgcat taccaatacc gaaggtggca gctttaacag
    10201 ccactacggt gtcaaagttt ttggcaacag ccacggcatg ttgcagggtt actgctcaac
    10261 gcgtcattcg ctctccagct gtgtaattgc cgaagaaaat ggcgatatgg agcgtgatta
    10321 cgcctacacc attggccgcg cgatgagcga tctgcaaacg ccagagtggg tcggggccga
    10381 ctgtgctcgc cgtactttat cgcgcctgtc accgcgtaaa ctctccacca tgaaagcgcc
    10441 agtcattttt gccaatgaag tggcaaccgg gctttttggc catctggtgg gggcgatagc
    10501 gggtggctcg gtttaccgta aatctacctt tctgctggat tcgctgggta aacaaattct
    10561 gccggactgg ctgaccattg aagagcatcc gcatctgctg aaagggctgg cgtcgacgcc
    10621 attcgacagc gaaggtgtgc gcaccgagcg tcgcgatatt attaaagatg gcatcctgac
    10681 tcagtggctg ctgaccagct actcggcgcg gaaactgggg ctgaaaagca ccggacatgc
    10741 gggcggtatt cacaactggc ggattgccgg acaagggcta agcttcgaac aaatgctcaa
    10801 agagatgggc accgggctgg tggtgacgga gctgatgggc cagggcgtga gtgccattac
    10861 cggtgattat tcccgtggtg cagcgggctt ctgggtagag aatggcgaaa ttcagtatcc
    10921 cgttagtgaa atcaccatcg caggtaattt aaaagatatg tggcgcaata ttgtcaccgt
    10981 cggtaacgat attgaaacac gcagtaatat acagtgtggt tctgtgctgt tgccggagat
    11041 gaaaatcgcc gggcagtaaa aaactggcgc gacctgccgc gctataaact aaaaaattac
    11101 acaaaatcat tcgcactgca tcgagacgga aagtgaatga gggccgggga gcgtactcgc
    11161 agtacgtgac ccgggcgaat gagcgtagcc gtcgatgagg cagtgtgaag gatgaagtgt
    11221 aaataaaaaa ggaagtgagc aatgcgtaaa agcctgttag ctattcttgc agtctcctcg
    11281 ttggtattca gttctgcgtc gtttgccgct gatcttgaag acaatatgga aaccctcaac
    11341 gacaatttaa aagtgatcga aaaagcggat aacgcggcgc aagtcaaaga cgcgttaacg
    11401 aagatgcgcg ccgcagcgct ggatgcgcaa aaagcaacgc cgccgaagct cgaagataaa
    11461 tcaccggaca gcccggaaat gaaagatttc cgccacggtt tcgacattct ggtcggtcag
    11521 attgacgacg cgctgaagct ggcaaatgaa ggtaaagtaa aagaagcgca ggctgctgca
    11581 gagcaactga aaacgacccg caacgcctat caccagaagt atcgttaatt cctcatttcc
    11641 ctgttgcctg cactcaggta acagggaact tatcatcgca aatgatgcac cacctgattg
    11701 ctgctgccgc gccagatcag ggaagggtct tttaaatcct gcacaaattt gccgtcgacc
    11761 agcacgttaa tcaaatcaac aacctgcatt tgcgcagcgt tgagttcgtc gagtttatag
    11821 cctgtccaca cccagatgtc tttacccgga cactcggcgc ggatgcgttt taccagtttc
    11881 agaatatccg gcacgttttg cggatgcagc ggatcgccgc cggagaggga aataccctgg
    11941 cgtttgatac gagtgtcatt cagatcgtta ataatctggt cttccattgc tttagtaaat
    12001 gtctgaccgg aatttacccg ccatgtgctt ttgttatagc aaccggggca ttcatgaaca
    12061 cacccggaga caaacagggt gcagcgagtg ccggggccgt tgacgatgtc gacaggatag
    12121 tactgatgat aattcattgt ttcgcctggc ttatcagtcc gcgtatagga tgcatccggt
    12181 aatcaatgcc tgatgcgacg ctgtcgcgtc ttatcaggcc tacagccgtt gccaaatgta
    12241 ggccggataa ggcgtttacg ccgcatccgg catttgctta gcgcagagtg aagattaacc
    12301 tatctgccca ttccccaaat gtttaacgcg gcgcttaact tcttcctgct taccagcgtt
    12361 aaatggacgt gcatccgggc tacctaaata tccgcacacg cggcgagtca ccgacacacg
    12421 ggaggcgtca tggttaccac atttcgggca agtgaagcct ttgctggtgc actcgaactc
    12481 accggtaaag ccacactcgt agcactcatc aatcggtgta ttggtgccgt aatacggtac
    12541 atgctgatag ctgtaatccc agacatcttc cagcgccttc aggttgtgct gaatgtttgg
    12601 atactcgccg tagcaaatga aaccaccgtt cgccagcggc gggtaaggcg cttcaaagtc
    12661 gatcttgtcg tacgggttca ccttcttctc cacatcgagg tggaaactgt tggtgtagta
    12721 acctttatcg gtcacgcccg gcaccacgcc aaactcagca gtatcgagac ggcagaagcg
    12781 gtcgcacagg ttttcactcg gcgtgctgta gagactgaaa ccataacccg tttcttcttt
    12841 ccactgatcc actgcctggc gcagacgttc aacaatggcg atacctttcg cgcgaagctg
    12901 ctcgttgtcg taaacatgct cgccgccgaa cagcgcgtta atggtttcgt ggatgccgat
    12961 gtaacccagc gaaatagacg cacgaccgtt tttgaagatt tcagaaacat catcgtcagc
    13021 attcagacgt acgccacaag caccttccat atagaggatc ggggcgacgc gcgctttcac
    13081 gccttcgaga cgagcgatac gggtcatcag tgccttacgt gccagcacca gacgttcatc
    13141 cagcagcttc cagaaggtgg cttcatcgcc ttttgcttcc agagcaatac gcggcaggtt
    13201 caggctgatc acgccgaggt tgttacgacc atcgtggatc tgctcgccgt tttcattttc
    13261 ccacacgccg aggaagctgc ggcagcccat cggggtttta aacgaaccgg tgactttcac
    13321 tacctgatcg tagttcagga tatccggata catgcgcttg cttgcgcact ccagcgccag
    13381 ctgtttgatg tcgtagttcg gatcgccttt tttatggttc aggccatcgc gaatcgcaaa
    13441 caccagtttc gggaacaccg cagttttacg gtttttaccg aggcctgcga tacggttacg
    13501 caggatcgat tcctgaatca ggcgcgattc ccagctggtg cccagaccaa aaccaaaggt
    13561 tacaaacggc gtctgaccgt tggcggtgtg cagggtgttt acttcgtact ccagcgactg
    13621 gaaggcatcg tagcactctt tgatggtacg agagttagcg tagccttcgg cgtccgggat
    13681 gttccactct tctgcggtct tgcgatgttt gttgtagctg gcggtaacaa acggcgccag
    13741 cacttcatcg atacggttaa tggtggtgcc gccataaata tggctggcga cctgagcaat
    13801 aatctgcgca gttaccgcgg ttgccgtaga gatcgacttc ggcggttcaa tctcggcgtt
    13861 ccccatttta aagccctggg tcagcatgcc tttcaggtcg atcaacatgc agttgaacat
    13921 cgggaagaac ggcgagtaat cgagatcgtg atagtgaata tcgccacgct catgtgcctg
    13981 caccacgtca cgcggcagca ggtgctggcg tgcatagtgt ttagccacga tcccggccag
    14041 caggtcgcgc tgggttggaa tcaccttgct gtctttgttg gcgttttcgt tgagtaacga
    14101 ggcgttggtc tgctcgacca gaccacggat ctcctggttc aggcgaccgc gtttttcacg
    14161 ttcaatgtcg cgatcgtgac ggtactcgat gtaagcacga gccagttgtt tgtatggacc
    14221 cgacatcagc tggttttcaa ctgcggtctg gatctcgttg atatccacct ggttgcggcc
    14281 ctgcatctgc tcgctgacaa ccgcggcaac agtggcgcaa taatcggcat catcgacttc
    14341 cgctgcttta gctgcacgca gaatcgcttc tttgatgcgc tctgatttaa acggcacttt
    14401 gcagccgtct cgtttcatca catgcggtgt catgatcgct ccatattttt aagaacaggt
    14461 tatccacaga aattgggaaa ggcgttttcc ggttttttcg tttcgttttc cggtgctttc
    14521 cgcaatcgcc atccgcttat ccacaacgcc gcaccttttt cgtggggagc tgtagtagca
    14581 attatagtcg attaatacaa catattgggt tgggacgcat tttaaagtct atatatagtg
    14641 ctttgcatca aggatgtttg aacttttttt gatgtagctc aaagtaaaaa gcagagcgta
    14701 cggatgacgg gcgctacagc gttatgtaaa ttttttaatg aatttgctgg ttgaaaaatc
    14761 aacaaaaaca acatgctgac agacaaaacc ccggaatgac cggcattacc ggggcttagg
    14821 gaagatttac ttctgtaacc accagacagc ctcaaaaggc cgtaaattca tggcacaggg
    14881 ttgtggtgag gcttcttcgt agttatgcat cacaagctgc cagttgccgc gcatttgccc
    14941 tggctgccag ggttggatct cacggctaag gttggcaatg accagcaagg tttgcccctt
    15001 ccattcacgg cgatagcacc acaatacagg gctgtttggc agcagatcct ggtaattgcc
    15061 ccatgtcagg atggcttctt gcttacgcag tgcgattaac ttttggtagg tgtaaaacac
    15121 cgaggactca tcggccagcg cggcttctac gttgatttgt tgatagttat cgcccaggcc
    15181 aatccacggt tcgccagccg taaacccggc attatcgccg ttgctccatt gcatgggcgt
    15241 gcggctgttg tcacgggatt tactggcgag gattgccaat aactcgtcgg catcacgacc
    15301 atcgttgcgc agctcggcaa acatattgag gctctccacg tcgcgatagt cagtaatgcg
    15361 cgtgaaatgc gggttggtca tgccaatctc ttcgccctgg tagatatacg gcgttccctg
    15421 catgccatgc agcaccatcg ccagcatttt tgccgcaggc acgcggtatt caccttcatc
    15481 accaaagcga gaaacaatgc gcggctgatc gtggttacac cagaacaagg cattccatgc
    15541 cacgttgtgc attccttgtt gccagtggcg gaacaatgtt ttcaacgcca caaagtcagg
    15601 tttagccagc gtccattttt caccaccggg ataatcgacc ttcaggtgat gaaaattaaa
    15661 ggtcatcgac aattcactgc ctgtcagtgc cgcgtatcgc tggcaatgct caaggctggt
    15721 ggaggacatt tcacctacgg tcattaaccc gcgtggcgta aacacatcgc ggttcatctc
    15781 gtgcaaaaac tcgtgtgctc gtggcccgtc ggtgtagaag cgacgcccgt cgccatccag
    15841 gtcttcaggg aaacgcgggt ctttggagat cagattcacc acatccaggc gcaacccgtc
    15901 gaccccacga tcggcccaga attcacagac ttttttcagc tctgcgcgta ccgctggatt
    15961 ctcccagttg agatccgcct gttctggtgc aaagagatgc aaatagtact gttcgctttc
    16021 cgcatgccag cgccacgcac taccgccaaa ttttgaacgc cagttgttcg gtggcgtttc
    16081 tggttcgcca tcgcgccaga tataaaactg gcggtaaggg ctttctttgt tcagcgcctc
    16141 gcgaaaccag gcatgttggg tagaggtatg gttaaacacc atatcgagaa tgatacgaat
    16201 cccgcgcgat tttgcctgcg tcaccagttc gtcaaaatcg tccagcgtgc cgtagatggg
    16261 atcaatcgcc gtatagttcg ctacgtcgta accgttatcg acctggggag agatataaaa
    16321 gggggttagc cagatggcat caacgcccag tttatgcaga tagtccaggc gttggataac
    16381 gccacgtaaa tcgccggtgc cgctacccgt ggtgtcctga aaactctttg gataaatctg
    16441 gtagataacg ccgttttgcc accagtgggg aagattagtc attacgttat tcctgcaaat
    16501 gcgagggggc gcaattgcgc cccgaagaaa attaaacaat atccagcgtg cccaggcggt
    16561 atttccgctg atagataaac gaggtgagta caatcgggat gatgatggcg ataaccattg
    16621 ccagcgcaaa tacctgccag tagctcggtt gaatcgagag aattcccggc aggccgccta
    16681 cgccgatacc attcgccata acgccgttca ggccgcatag taatcctgcc agaccagaac
    16741 caatcatcgc gcacagcatc gggaagcgat atttcaggtt gatgccgtac attgcaggct
    16801 cagtgacccc aagccaggcg gagatagcgg caggcacgga gatctcgcgt tcattgtgct
    16861 tgcggctgga aattataatg cctatcacgg cggagccctg agcgatattc gacagcgcaa
    16921 tcagcggcca cactggcgtg ccacccatgc tttgaatcat ctgcaaatca atcgcaaggg
    16981 tggtctggtg tacaccggtg atcaccagcg gggcgtacag gaagccaaac aatgctgcgc
    17041 caatcggagc aaagctgccg gtcatcaggt gacgtaccgc aaaggcaacg ccatcgccaa
    17101 tcatgcgacc aaacggacca atcagcgcat gggcgaggaa caccgcgagg atcagcgaac
    17161 agacgggcac caccaccaga tagaggtaat ccggcacgat gcgtttaagg cgagtttcaa
    17221 taacgcccag cgccagcccg gctaacagtg ccgggatcac ctgcgcctga tagcctactt
    17281 tggcgatgct gaacatgcca aagtcccaca cttccggcag ctgctgcccg agcagataag
    17341 cgttcatcag ctggggagaa accagtgtca cgccaagcac gataccaagg atcggcgtgc
    17401 cgcccatttt tttcaccgct gaccagcaaa tcccgaccgg caggtagaag aagatcgctt
    17461 caccgatcaa ccacagaaaa tcgtagatcg tttgcaggga agggtacatt tgcgccagcg
    17521 tttgaccgtt gctcatgggc aaatcgccga tcacattgcg aaaaccgagg atcaaaccgc
    17581 cgctaatcaa cgcgggcagc aacgggaaga agatctccgc gaaatgagag atcaactgct
    17641 catgccattt catattctgc cgggcggctt tttttacctg ctctttatca acctgcgcct
    17701 gtccggttga cgctattagt gcttgatagt aatcacccac gttggtgcca atcaccacct
    17761 gaaattgccc ggcattggtg aaacagcctt tcaccatggg gagttgctca atttctttcg
    17821 gtctggcatt ggccggttgg ttgaggacaa agcgcaggcg agtaatacag tggctcaccg
    17881 tcgcaatatt gccgcgcccg ccgaccagtt caatcaaccg atcgatatcc gtttggttta
    17941 ttttgctcat cataaagccc catggcagat gacatttttg gttggctgca gaatatattg
    18001 cgcggaaaaa tttaaaaacg ggaacgttcc cgaaacgcag cgaagatcac aatttatcgt
    18061 tcaggaaacg atcaggacag ggtggcgggg atgatgattt gttgcggttc gctgcgcccg
    18121 gttacctgtg cgatcaattg gcaggccgcc tggcgtccag cttcggcgta accaggatct
    18181 acggtgacga tctccggatg aaggaatttc attaatggcg tattaccgac gctcgccagt
    18241 tgcaaggtgt cgatgcgttg ctcttgcagg tatttacttg cgccaagtgc cagcgtgtcg
    18301 gttgcgcaca gtaaggcggt agtttcaggc gtaatcactt ttgctacgtt ttcatagcct
    18361 tgcttcatag caagccctgg cagagcggca acgggatgca gtttatgcgc tttgcagaac
    18421 gccaggtagg cttcgtgacg tcgcttcccg gtcgtcacgt cactatgcgg cacgccgaga
    18481 taactgatat tgcgatgccc ctggtcatag agccgttgca tcaggatctt gattgcccct
    18541 tcgtcgtcat aacagaccga agcaaagcct tttgcgtcac gcgccagcag aaccagcgat
    18601 gactgccagt gggctaacat ttcttctgtt atgccagtaa aaccgaacaa cactacgccg
    18661 tcgatattac gccgtttcag cacccccaaa tgttcggcaa ctaattgcgg ggaaaactga
    18721 ctttccatca tgattgggtc gtaaccttgt tcatagaacg ctggcagcat ggtttgaaca
    18781 gcgagatttt ctgacaacga atccaggcgg gtaacaatga tggcgaccac tttatcgctt
    18841 tgcccacgca tagcgcgcgc agagcgggaa ggggaaaatc catgctgatt catcactgct
    18901 tcaacacgct cgcgggtgcg ctggctcacg ccgctttcgt tattcagcac ccgggaaacc
    18961 gtagatttcc ccacgccgct taagcgcgcg atgtctttga tggtcagccg attttgcatc
    19021 ctgttgtcct gtaacgtgtt gtttaattat ttgagcctaa ctttacccgt gcattcagca
    19081 atgggtaaag tctggtttat cgttggttta gttgccagca ggtattatat cgccatagat
    19141 gctacgaata ttattggatt ctccttatta tttgcggcgc ttttttcact taccggaggt
    19201 tatatggaac ctgatcccac gcctctccct cgacggagat taaaactttt ccggtaagcc
    19261 cgtcttttca cggcgttacc ggatgcgtaa ggccgtgacg ttttaacgtc cctgctcagc
    19321 tttattacct tcaggtaagg cttcgccacg cctgaagaca tttctgtact gtttcagaca
    19381 gtgcggaggg actccttatg tttaaagaaa tttttacccg gctcattcgc catttacctt
    19441 cccgtctggt tcatcgcgat ccattgcctg gcgcgcagca gacagtgaat acggcggtcc
    19501 cgccgtcctt aagtgcgcat tgcctgaaaa tggcggtgat gcccgaagaa gaattatgga
    19561 aaacgttcga cacccatccg gaagggttaa atcaggcgga agtggaatct gcccgcgaac
    19621 aacatggtga aaataaatta cccgcacaac aaccgtcgcc gtggtgggta catttatggg
    19681 tctgctatcg caaccccttt aatattttac tcaccattct cggcgctatt tcttacgcca
    19741 cggaagattt atttgccgcg ggcgttattg cgctaatggt cgctatttca acgttactga
    19801 actttattca ggaagcacgt tccactaaag cggcagatgc cctgaaagcg atggtcagca
    19861 ataccgcgac ggtgctgcgc gtaattaacg acaaaggcga aaatggctgg ctggagatcc
    19921 cgatcgacca gctggtgccc ggcgatatta taaaactggc ggcgggagat atgatcccgg
    19981 cagatttacg tatcttgcag gcgcgggatc tgttcgtcgc tcaggcgtcg ttaaccggtg
    20041 agtctctgcc cgtggaaaaa gccgctacca ctcgccagcc ggagcacagc aatccgctgg
    20101 agtgcgacac gctgtgtttt atgggcacca ccgtggtgag cggcacggca caagcaatgg
    20161 tgattgctac aggtgccaat acctggtttg gtcaactggc ggggcgtgtt agtgagcagg
    20221 aaagcgagcc gaatgccttt cagcagggga tcagccgcgt cagtatgctg ctgattcgct
    20281 ttatgctggt gatggctccg gtggtgttgc ttatcaacgg ttacaccaaa ggcgactggt
    20341 gggaagcggc actgtttgcg ctttcggtcg cggtaggctt aacgccggaa atgttgccga
    20401 tgattgttac ctcgacgctg gcgcgcggcg cagtaaagct gtcgaaacag aaagtgatcg
    20461 tcaaacatct ggatgctatt cagaactttg gcgcaatgga tattctgtgc actgataaaa
    20521 ctggcaccct gacacaggac aaaattgtgc tggagaatca taccgatatc tccggtaaaa
    20581 ccagcgaacg cgtgctgcat agcgcgtggt tgaacagtca ttatcagacc ggacttaaaa
    20641 acctgctcga tacagcggtg ctcgaaggta cggatgaaga gtcagcgcgc tcgctggcca
    20701 gtcgttggca aaaaattgat gagattccgt ttgatttcga gcgtcgccgg atgtcggtgg
    20761 tagtggcaga aaataccgag caccatcagc tggtttgcaa aggtgcattg caggaaatcc
    20821 tcaatgtgtg ttcgcaggtg cgtcacaatg gcgagattgt gccgctcgat gacatcatgc
    20881 tgcgtaagat taagcgggtt actgatacgc ttaatcgtca ggggctgcgc gtggttgcgg
    20941 tggcgacgaa atacctgcca gcgcgtgaag gagattacca gcgggcggat gaatccgacc
    21001 tgatcctcga aggatatatt gctttccttg atccgccaaa agagacaacc gctccggcac
    21061 tgaaggcatt aaaagcgagt ggaatcaccg taaaaatcct tactggcgac agtgagttag
    21121 ttgcagcgaa agtgtgccat gaagtggggc tggacgcggg agaggtggtc attggtagtg
    21181 atattgaaac gctatctgac gacgaactgg caaatctcgc acagcgcacc acgctgtttg
    21241 cccgcctgac gccgatgcat aaagaacgca ttgtgacctt actgaagcgc gaagggcatg
    21301 tggttggctt tatgggcgat ggtattaatg atgcgcccgc cttacgcgct gcggatatcg
    21361 gcatttctgt ggacggcgcg gtagatattg cccgtgaagc ggctgatatc atcctgctgg
    21421 aaaaaagcct gatggtgctg gaagaggggg ttattgaagg acgtcgcact ttcgccaaca
    21481 tgctgaaata catcaaaatg acggcgagct ccaacttcgg taatgtgttc agcgtgctgg
    21541 tggcgagtgc tttcttgccc ttcctgccga tgttgccgtt acacttgctt attcagaacc
    21601 tgctgtatga tgtgtcacag gtggcgatcc cgtttgataa cgtcgacgac gagcaaattc
    21661 aaaagccgca gcgttggaat ccggcggatc tggggcgctt tatgatcttc ttcggaccga
    21721 tcagctcgat cttcgatatt ctgacgtttt gcctgatgtg gtgggtattc catgccaaca
    21781 cgccggaaac gcaaacgctg ttccagtcgg gatggtttgt ggtgggctta ctgtcgcaaa
    21841 cgctgattgt gcatatgatc cgcacccgcc gtgtgccgtt tattcagagc tgtgcatcgt
    21901 ggccgttaat gatcatgacc gtgatcgtga tgattgtcgg gatcgcattg ccgttttcac
    21961 cgctggccag ttatctgcag ctgcaggcgc tgccgttaag ctatttcccg tggctggttg
    22021 cgattctggc agggtatatg acattaactc agttggtgaa aggattttat agccgtcgtt
    22081 acggctggca gtaaagaata aactgggcac gatagccccg attcagattg cagacaaagt
    22141 aagctttgtt catgccggat gcggcgtaaa tgccttatcc ggccaacaaa ttcaataaat
    22201 tgcaggaatc acgtaggcct gataagcgta gcgcatcagg cgattttgcg tttgtcatca
    22261 gacttaatcc gggcatgata gcccggattt ccatcaagat tagcgacgaa cagcgatcgc
    22321 ttcgatctca atcttcacgt ctttcggcag acgggcaact tcaacgcaag aacgtgccgg
    22381 gaaggtggca ttgtgttcgg tgaagaaggc ttcgtaggta gcattaacgg ttgcgaagtc
    22441 gttcagatct ttcacaaaca cggtggtttt aacgatgtcg cccactttca ggcctgcggc
    22501 ttcgacgatc gcttttacgt tatccagcga ctgacgtgcc tgtgcagcga cgtctgccgg
    22561 tacttcgccc gttttcggat ttaccgggat ctggccggag gtgatgatca tattgcccag
    22621 atcaacgccc tgtacgtaag gaccgatagc tgccggtgca ttttccgtcg cgatagtttt
    22681 gctcatgatt tctcctttat tacagcggta aaaagtctgg ctcattatag ggagccagac
    22741 ttttattacc aaccgcaatt aattggccag caccacatta tgggaaaact ctttttcaca
    22801 gtatttgcat ttgagcgcga tgtcattggc gcgttttcgc acggcaaagc tggaagaaac
    22861 cggttcggca tggctgatac agttgctgtt cgggcagacc agcacattgt cgatgcgctc
    22921 tggcagactg gggcgcgatt tacccaccac ttcatagttg tcgatacggt taaccgtggc
    22981 ttgcggcgca tacaatgcca gttgatctac ctgatcttca ctcaaaaagg tattttcgat
    23041 tttgatcaga tctttgcggc ccatctcgcc agaaggcaag ttcagaccga tggtgatgcg
    23101 ctgatccgtt tcggtcagct tgaacagact caacagctta aaaccgatct gggcggggat
    23161 atggtcaatt accgtgccgc gtttaatagc ttcaacctgc aatttattat cgtgtgtcat
    23221 ctctatttcc ccttacagta ccagatcgcg attcagaacc agtgccagta acgcctggcg
    23281 agcgaaaatc ccgttgcctg cctgctggaa gtaccaggcg tgtggcgttt tatcaacatc
    23341 cgtcgcaatc tcatcaacac gcggcagcgg atgcagcact ttcatattgg ctttggcgtt
    23401 gtggagatcg ctggcgcgaa gaacaaactg cgctttcacg ttggcgtact cggacgggtc
    23461 cagacgctct ttttgcacgc gggtcatgta caggatgtct acttccgcta tcacttcttc
    23521 aatagagctg tgcagactcc atgcgatccc tttttcatcg agcatatcca gaatgtattg
    23581 cggcattgcc agcgcgtccg gcgcgatgaa gtaaaaacgg ttgccgtcga acttcgctaa
    23641 cgcctgagtc agggagtgaa cggtgcggcc atatttcagg tcaccaacca ttgcgacgtg
    23701 gagattgtcc agacgcccct gggtttcctg aatagtgaat aagtccagca aggtttgcgt
    23761 cggatgttgg ttggagccat caccggcatt cagtaccggt acattgccgg aaaactcggt
    23821 ggccaggcgc gccgcacctt cctgcggatg acgcatcact atcgcatcga cgtaagtgct
    23881 gataaccgaa atggtatcgg ccagcgtttc gcccttttta cccagtgatg tattggcgct
    23941 gtcggagaag cccactacgc tggcacccag gcggtgcata gatgtttcga aagagaggcg
    24001 ggtacgggta gaggcttcga agaaacagct ggcaatgact ttgtgcttca acagctctgg
    24061 ttgcgggttt gctttcagtt tcgccgctgt cgccagcacc agattaaggt catcgcgact
    24121 aaggtcgttt atggaaatga tatgtttctg atatagcgga ttagccatct tttatctcct
    24181 gacgcctggg caaaaaaaag cccctcgatt gaggggctgg gaatgggtga tcaacgggaa
    24241 gaaaaacggc aggccagcgt cttttttcag acgcggtaag acaaaatgtc gaacacactg
    24301 aaccatacat cctcccggca aattgtccgg cattatactc atcgtcagaa gcggtgcaag
    24361 catttgatgc aatattttgt cagcgcaaac ggtttatttg aattataagt caaaatacat
    24421 gcatttttat ttgttgtgat tctgcagggg aacggtcgtt cggtatcaat aattgcagac
    24481 atttcctgct tttttctttt tcactttcac gcaatcagat atgcatttta ttccattcat
    24541 ccgacttata gggcggagtg tgaaaacgaa cggctaacac tattgcttac tgctcaggga
    24601 tgcgcgctat cactttaatt tcaaaatcaa agcctgccag ccatgtaaca cctaccgccg
    24661 tccagtttgg ataaggtggg gcgctaaata tttcattttt caccgtcatg atgtcttcaa
    24721 attggttttc tggatcggta tggaagctcg taacatcaat gatatcgtca aaagtgcatc
    24781 ccgcagcagc cagggtcgca tgcaaattat caaatgccag tctgacttgt tgctgaaaat
    24841 cgggttctgg tgttccgtcc tctcgacttc ctacttgccc ggaaacaaac agcaaatcgc
    24901 cggagcgaat agccgcagaa taacgatgtt cagcatacag tgaatgtcgg ccagcaggga
    24961 aaacagcggt tctttctacc atttggttat cctcaagatt tacgacatga acagaagatt
    25021 tatatttacc aggagccgct tttagcggac gacgtgagta aacaaaaccc agacatcatg
    25081 ggaaatggct gggcttaatt gagcgtagtc ggttatgcgc caaacgcgcc atcaatggta
    25141 tgcatcgcgc cggtaacaaa gctggcttct ggccctgcta accatgcgac cataccagcg
    25201 acctcttccg gttgcccatg tcttttgata gccataaaac catgcaacat atcgcgcatt
    25261 ggcccgttgg cgggattagc gtcggtatca attggccctg gctggacgac gttgatggtg
    25321 atcccacgcg gtccaaaatc acgggccagc ccgcgcgcca tgccttgcag ggcagatttg
    25381 ctggcggcat aagcagccat gcctgcaaca ggcatacgat cgccattcac ggagccgatg
    25441 attaagatgc gcccgccttc gggcatctgc cgggcggctt caacggaggc atggtaagga
    25501 gcatgaatat tgattttgaa aaggcgatca atatcgtcgg catttaattc cagggcatcg
    25561 ccaaagacgc caatacctgc atttaccacg tggatatcca atgcgccgct cttacgaacg
    25621 acatcaataa cagcgtctct gtcagcacta tctgtgaata ctgctgtcgc tcccgtctct
    25681 tgtgccaggc gttcagcggc atctttcgac cccgcatagg tgaatcgtac attggcccca
    25741 tcggtgacga aacgacgtac gatagcggca ccgattccac ggctaccacc gaggatgaga
    25801 actgtcttac ctgtaaaagc gcccataagg actccttgat ttattatgta acatgcatta
    25861 caaaactgtt ttaactttct gtcaacaagt tttgtaatgg tcactaaaaa acaatctcgc
    25921 gttccaggtc gtcccagacg tttcgctcct gagcaggcag tctctgcggc aaaagtgctt
    25981 tttcaccaaa aaggtttcga tgctgtcagt gttgctgaag ttactgatta tcttggtatt
    26041 aaccccccga gcctctacgc ggcttttggc agtaaggctg ggttatttag ccgtgtactc
    26101 aatgaatatg tcggtacgga agctattccg cttgccgata ttcttcttga tgatcgtccg
    26161 gtaggcgagt gcctggttga ggtattaaaa gaagcggcgc gcagatatag ccaaaacggc
    26221 ggctgcgctg gctgtatggt tcttgaaggt attcatagtc atgatccaca agcgcgtgat
    26281 attgccgttc aatattatca cgccgcagaa acgaccattt atgactatat cgccaggcgg
    26341 catccacaaa gcgcacaatg tgtgactgat tttatgagta ccgtgatgtc agggctttct
    26401 gcgaaggcac gggaggggca ctccatcgaa caactctgtg caacagctgc actggcgggg
    26461 gaagcgataa aaactattct caaggagtga tgcgggcctt gacccgaaag gcgggaacgc
    26521 gcctgccgat aagttgtgat aagacaataa ttcacgcgtt aaggctagcg gaattgatca
    26581 tcttttcgta taacgataga aatgaaacgt tgttttaatt aaggagtgga aaagatgatc
    26641 atcggaaata ttcataatct tcagccgtgg ctaccccagg agttacgcca ggcgattgag
    26701 catatcaaag cacacgttac ggtagaaacg ccaaagggca agcacgatat cgaaggcaat
    26761 cgtctgtttt atcttatctc ggaagatatg accgagccgt acgaagctcg ccgtgcggag
    26821 taccatgccc gctatctcga cattcagatt gtgttaaaag gtcaggaagg catgaccttc
    26881 agcacacaac ctgcaggtac gccggatacc gactggttag ctgataaaga catcgcattt
    26941 ttgccggaag gcgttgatga gaaaacagtt atccttaatg aaggtgattt tgttgtgttt
    27001 tatccggggg aagtgcataa accgctgtgc gcagtgggtg caccagccca ggttcgcaaa
    27061 gcagtagtga agatgctgat ggcgtgatga cttttcgccg taaataactc caggtttacg
    27121 gcgagtttgt gaaaagagcg ttttttgata ttttttgtga gtaaaatttg taatgcttag
    27181 acgttcttat taactcaagg agtccgtcat gagcaaaata tcaggttgga atttttctca
    27241 aaacattaca tcagccgaca attgtaaaca aaaaaatgaa gacttagata cctggtatgt
    27301 gggaatgaat gattttgccc gaattgccgg agggcagaat agcagaagca atattctttc
    27361 tcccagagca tttttggagt ttttggctaa gatatttacc ctgggttatg tggattttag
    27421 caaacgctcc aacgaagcgg gtagaaatat gatggctcat aataagtcct catcttatat
    27481 caaaaataat gatggtagtg agataatgaa gtttgttatg aataatcctg aaggggagcg
    27541 agcggattta tctaaggtgg aaatagaaat tacacttgcc tcagctttta ataatgggat
    27601 tcgtgaagga catacagtca ttatatttac acaacctgat ggttcgacta atcgttatga
    27661 agggaagtcc tttgaaagaa aagatgagag ttcattacac ctgattacta acaagattct
    27721 ggcgtgttac caacgcgaag ctaa
//
LOCUS       DAZFQD010000060        27322 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.060, whole genome shotgun sequence.
ACCESSION   DAZFQD010000060 DAZFQD010000000
VERSION     DAZFQD010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27322
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.060"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            115..1335
                     /gene="ybdN"
                     /locus_tag="ACHKTW_002595"
     CDS             115..1335
                     /gene="ybdN"
                     /locus_tag="ACHKTW_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HFO6463598.1"
                     /translation="MSIYKIPLPLNILEAARERITWTLNTLPRVCVSFSGGKDSGLML
                     HLTAELARRMGKKICVLFIDWEAQFSCTINYVQSLRELYTDVIEEFYWVALPLTTQNS
                     LSQYQPEWQCWEPDVEWVRQPPQDAITDPDFFCFYQPGMTFEQFVREFAEWFSQKRPA
                     AMMIGIRADESYNRFVAIASLNKQRFADDKPWTTAAPGGHSWYIYPIYDWKVADIWTW
                     YANHQSLCNPLYNLMYQAGVPLRHMRICEPFGPEQRQGLWLYHVIEPDRWAAMCARVS
                     GVKSGGIYAGHDNHFYGHRKILKPEHLDWQEYALLLLNSMPEKTAEHYRNKIAIYLHW
                     YQKKGIEVPQTQQGDIGAKDIPSWRRICKVLLNNDYWCRALSFSPTKAKNYQRYNERI
                     KGKRQEWGILCNND"
     gene            1308..1936
                     /gene="ybdM"
                     /locus_tag="ACHKTW_002596"
                     /pseudo
     CDS             1308..1936
                     /gene="ybdM"
                     /locus_tag="ACHKTW_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308667.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA];
                     GO_process: GO:0043170 - macromolecule metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043412 - macromolecule modification
                     [Evidence IEA];
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IbrB-like domain-containing protein"
     gene            complement(1937..3097)
                     /gene="ybdL"
                     /locus_tag="ACHKTW_002597"
     CDS             complement(1937..3097)
                     /gene="ybdL"
                     /locus_tag="ACHKTW_002597"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HFO6463599.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTQMSALAQQHQAINLSQGFPDFDG
                     PRYLQERLAYHVAQGANQYAPMTGVQALREAIAQKTERLYGYQPDADSDITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYSPAIALSGGIVKRMALQPPHFRVDWQEFAALL
                     SERTRLVILNTPHNPSATVWQQADFAALWQAIAGHEIFVISDEVYEHINFSQQGHASV
                     LAHPQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLA
                     LADMLRAEPEHYLALPDFYRQKGDILVNALNESRLEILPCEGTYFLLVDYSEVSTLDD
                     VEFCQWLTQEHGVAAIPLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
     gene            3206..4294
                     /gene="hcxA"
                     /locus_tag="ACHKTW_002598"
     CDS             3206..4294
                     /gene="hcxA"
                     /locus_tag="ACHKTW_002598"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HFO6463600.1"
                     /translation="MPHNPIRVVVGPANYFSHPGSFNHLHDFFTDEQLSRAVWIYGKR
                     AIAAAQTKLPPAFGLPGAKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAVPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQQYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALS
                     DQQNQQLTQSFCDVVDAIIAGGGMVGGLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTGAYQRFHLPTTLAELEVDINNHAEIDKVITHTLRP
                     VESIHYLPVTLTPDTLRAAFEKVESFKA"
     gene            complement(4304..4501)
                     /gene="ybdD"
                     /locus_tag="ACHKTW_002599"
     CDS             complement(4304..4501)
                     /gene="ybdD"
                     /locus_tag="ACHKTW_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFO6463601.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(4677..6782)
                     /gene="cstA"
                     /locus_tag="ACHKTW_002600"
     CDS             complement(4677..6782)
                     /gene="cstA"
                     /locus_tag="ACHKTW_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter CstA"
                     /protein_id="HFO6463602.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPTRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLAIFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFSITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQGVVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTAGWQKAFSPDAKVGFLAIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKTALAALKEPKPTAKETP
                     YEPMPENVEEIVAQAKGAH"
     gene            complement(6963..7376)
                     /gene="entH"
                     /locus_tag="ACHKTW_002601"
     CDS             complement(6963..7376)
                     /gene="entH"
                     /locus_tag="ACHKTW_002601"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HFO6463603.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTRLGDDVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPVSEGKVRGVC
                     QPLHLGRQNQSWEIVVFDEQGRRCCTCRLGTAVLG"
     gene            complement(7379..8125)
                     /gene="entA"
                     /locus_tag="ACHKTW_002602"
     CDS             complement(7379..8125)
                     /gene="entA"
                     /locus_tag="ACHKTW_002602"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
                     EntA"
                     /protein_id="HFO6463604.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALAFVEAGAKVTGFDQAFTQEQY
                     PFATEVMDVADAGQVAQVCQRLLAETERLDVLINAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTPRIGMSAYGASKAALKSLVLSVGL
                     ELAGSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            complement(8125..8982)
                     /gene="entB"
                     /locus_tag="ACHKTW_002603"
     CDS             complement(8125..8982)
                     /gene="entB"
                     /locus_tag="ACHKTW_002603"
                     /EC_number="3.3.2.1"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HFO6463605.1"
                     /translation="MAIPKLQAYALPESHDIPKNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEELLPAPVPASKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            complement(8996..10606)
                     /gene="entE"
                     /locus_tag="ACHKTW_002604"
     CDS             complement(8996..10606)
                     /gene="entE"
                     /locus_tag="ACHKTW_002604"
                     /EC_number="2.5.1.-"
                     /EC_number="6.2.1.71"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase EntE"
                     /protein_id="HFO6463606.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GERQLSYRELNQAADNLACSLRRQGIKPGETALVQLGNVSELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVTEHSSIRVVQLHNDSGE
                     HNLQDAINHPAEDFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSVEIC
                     QFTQQTRYLCAIPAAHNYAMSSPGSLGVFLAGGTVVLAADPSATLCFPLIEKHQVNVT
                     ALVPPAVSLWLQALTEGESRAQLASLKLLQVGGARLSATLAARIPAEIGCLLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEEIENL
                     LLRHPAVIYAALVSMEDELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLRQWLASRASA"
     gene            complement(10616..11791)
                     /gene="entC"
                     /locus_tag="ACHKTW_002605"
     CDS             complement(10616..11791)
                     /gene="entC"
                     /locus_tag="ACHKTW_002605"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
                     /protein_id="HFO6463607.1"
                     /translation="MDTSLAEEVQQTMATLAPNRFFFMSPYRSFTTSGCFARFDEPAV
                     NGDSPDSPFQQKLAALFADAKAQGIKNPVMVGAIPFDPRQPSSLYIPESWQSFSRQEK
                     QTSARRFTRSQSLNVVERQAIPEQTTFEQMVARAAALTATPQVDKVVLSRLIDITTDA
                     AIDSGVLLERLIAQNPVSYNFHVPLADGGVLLGASPELLLRKDGERFSSIPLAGSARR
                     QPDEVLDREAGNRLLASEKDRHEHELVTQAMKEVLRERSSELHVPSSPQLITTPTLWH
                     LATPFEGKANSQENALTLACLLHPTPALSGFPHQAATQVIAELEPFDRELFGGIVGWC
                     DSEGNGEWVVTIRCAKLRENQVRLFAGAGIVPASSPLGEWRETGVKLSTMLNVFGLH"
     gene            12166..13122
                     /gene="fepB"
                     /locus_tag="ACHKTW_002606"
     CDS             12166..13122
                     /gene="fepB"
                     /locus_tag="ACHKTW_002606"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HFO6463608.1"
                     /translation="MRLAPLYRNALLLTGLLLSGIAAAQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSAEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQSLLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIIQLGGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYALGTETFRLDYYSAMQVLERLKALF"
     gene            complement(13126..14376)
                     /gene="entS"
                     /locus_tag="ACHKTW_002607"
     CDS             complement(13126..14376)
                     /gene="entS"
                     /locus_tag="ACHKTW_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042931 - enterobactin transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042930 - enterobactin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HFO6463609.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWQVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATPALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPALPPPPQPREHPLKSLLAGFR
                     FLLASPLVGGIALLGGLLTMASAVRVLYPALADNWQMSAAQIGFLYAAIPLGAAIGAL
                     TSGKLAHSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYT
                     MLQTQTPEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGV
                     LLLLVLVELRRFRQTPPQVTASDS"
     gene            14487..15491
                     /gene="fepD"
                     /locus_tag="ACHKTW_002608"
     CDS             14487..15491
                     /gene="fepD"
                     /locus_tag="ACHKTW_002608"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HFO6463610.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAL
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFTGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLIAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            15488..16480
                     /gene="fepG"
                     /locus_tag="ACHKTW_002609"
     CDS             15488..16480
                     /gene="fepG"
                     /locus_tag="ACHKTW_002609"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HFO6463611.1"
                     /translation="MIYVSRRLLITCLLLISACVVAGIWGLRSGAVTLETSQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALAAMVGGIVTSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAAALLVRQMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHISRRISGTARW
                     GLTQAALCGALLLLAADLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            16477..17292
                     /gene="fepC"
                     /locus_tag="ACHKTW_002610"
     CDS             16477..17292
                     /gene="fepC"
                     /locus_tag="ACHKTW_002610"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HFO6463612.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTRWRKEDEEAVTKAMQATGITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGTPLVVPLGRTAPSTANS"
     gene            complement(17289..18422)
                     /gene="wzz(fepE)"
                     /locus_tag="ACHKTW_002611"
     CDS             complement(17289..18422)
                     /gene="wzz(fepE)"
                     /locus_tag="ACHKTW_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator Wzz(fepE)"
                     /protein_id="HFO6463613.1"
                     /translation="MSSLNIKQGSDAHFSEYPLASPSNNEIDLLNLIEVLWRAKKTVM
                     AVVFAFACAGLLISFILPQKWTSSAVITPAEAIQWQDLEKTFTKLRVLDLDVNIDRGG
                     AFNLFIKKFQSVSLLEEYLRSSPYVMDQLKEAKIDELDLHRAIVALSEKMKAVDDNAS
                     KKKDEPSLYTSWTLSFTAPTSKEAQTVLSGYIDYISALVVKESIENVRNKLEIKTQFE
                     KEKLAQDRIKTKNQLDANIQRLNYSLDIANAAGIKKPVYSNGQAVKDDPDFSISLGAD
                     GIERKLEIEKAVTDVAELNGELRNRQYLVEQLTKANINDVNFTPFKYQLSPSLPVKKD
                     GPGKAIIVILSALIGGMVACGSVLLRYAMASRKQDAMMADHLV"
     gene            complement(18638..22519)
                     /gene="entF"
                     /locus_tag="ACHKTW_002612"
     CDS             complement(18638..22519)
                     /gene="entF"
                     /locus_tag="ACHKTW_002612"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0031177 - phosphopantetheine binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HFO6463614.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSDLPSAWSVAHYVELTGDVDAPLL
                     ARAVVVGLAQADTLRMRFTEDNGEVWQWVDDALIFELPEIIDLRTNIDPHGTAQALML
                     ADLQQDLRVDSGKPLVFHQLIHVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCTW
                     LRGEPTPASPFTPFADVVEEYQQYRESEAWQRDAAFWAEQRRQLPPPASLSPAPLAGR
                     SASADILRLKLEFTDGEFRQLATQLSGVQRTDLALALAALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTASGPVLNVLPLGIHIAAQETLPELATRLAAQLKKMRRHQRYDAEQIVRD
                     SGRAAGEEPLFGPVLNIKVFDYQLDIPGVQTQTHTLATGPVNDLELALFPDEHGDLSI
                     EILANKQRYDEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQINATQV
                     EIPETTLSALVAEQAAKTPDAPALADARYLFSYREMREQVVALANLLRERGVKPGDSV
                     AVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQLPRF
                     SDVPNLTSLCYNAPLTPQGSAPLQLSQPHHTAYIIFTSGSTGRPKGVMVGQTAIVNRL
                     LWMQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAMQQFF
                     AEYGVTTTHFVPSMLAAFVASLTPQTARQNCATLKQVFCSGEALPADLCREWQQLTGA
                     PLHNLYGPTEAAVDVSWYPAFGEELAQVRGSSVPIGYPVWNTGLRILDAMMHPVPPGV
                     AGDLYLTGIQLAQGYLGRPDLTASRFIADPFAPGERMYRTGDVARWLDNGAVEYLGRS
                     DDQLKIRGQRIELGEIDRVMQALPDVKQAVTHACVINLAAATGGDARQLVGYLVSQSG
                     LPLDTSALQAQLRETLPPHMVPVVLLQLPQLPLSANGKLDRKALPLPELKTQASGRAP
                     KAGSETIIAAAFASLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQFARQVTPGQVM
                     VASTVAKLATIIDGEEDSSRRMGFETILPLREGNGPTLFCFHPASGFAWQFSVLSRYL
                     DPQWSIIGIQSPRPHGPMQTATNLDEVCEAHLATLLEQQPHGPYYLLGYSLGGTLVQG
                     IAARLRARGEQVAFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGG
                     TSTELFTTIEGNYADAVRLLTTAHSVPFDGKATLFVAERTLQEGMSPERAWSPWIAEL
                     DIYRQDCAHVDIISSEAFEKIGPIIRATLNR"
     gene            complement(22516..22734)
                     /gene="ybdZ"
                     /locus_tag="ACHKTW_002613"
     CDS             complement(22516..22734)
                     /gene="ybdZ"
                     /locus_tag="ACHKTW_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis protein YbdZ"
                     /protein_id="HFO6463615.1"
                     /translation="MAFSNPFDDPQGAFYILRNAQGQFSLWPQQCALPAGWDIVCQPQ
                     SQASCQQWLEVHWRTLTPANFTQLQEAQ"
     gene            complement(22737..23939)
                     /gene="fes"
                     /locus_tag="ACHKTW_002614"
     CDS             complement(22737..23939)
                     /gene="fes"
                     /locus_tag="ACHKTW_002614"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0008849 - enterochelin esterase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HFO6463616.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFWWRDPQGSEEYS
                     TIKRVWIYITGVTDHHQNSQPQSMQRIAGTDVWQWTTQLNANWRGSYCFIPTERDDIF
                     SAPSPDRLELREGWRKLLPQAIADPLNPQSWKGGRGHAVSALEMPQAPLQPGWDCPQA
                     PEIAAKEIIWKSERLKNSRRVWIFTTGDATAEERPLAVLLDGEFWAQSMPVWPVLTSL
                     THRQQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPLVKAIAPFSDRADRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRGGQQEGVLLEKLKAGEVSAEG
                     LRIVLEAGIREPMIMRANQALYAQLHPIKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            24182..26422
                     /gene="fepA"
                     /locus_tag="ACHKTW_002615"
     CDS             24182..26422
                     /gene="fepA"
                     /locus_tag="ACHKTW_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HFO6463617.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLD
                     KTQADAWDINQGHQSARAGTYATTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDAYTRSKYGDETNRLYRQNYALTWNGGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKATQDFVDIDLDDVMLHSEVNLPIDFLVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGANTGGAIDGVSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKMGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASAGGCYLQGNDDLKAETSINKEIGLEFKRDGWLAGVTWFRNDYRNKIEAGYVAVG
                     QNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSETVMWTNNITYMLKSENKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATWDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SVNTHF"
     gene            26462..26560
                     /locus_tag="ACHKTW_002616"
                     /pseudo
     CDS             26462..26560
                     /locus_tag="ACHKTW_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            26597..27217
                     /gene="entD"
                     /locus_tag="ACHKTW_002617"
     CDS             26597..27217
                     /gene="entD"
                     /locus_tag="ACHKTW_002617"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009366 - enterobactin synthetase complex
                     [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009239 - enterobactin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HFO6463618.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFDPANFCEQDLLWLPHYAQLQHAGR
                     KRKAEHLAGRIAAIYALREYGYKCVPAIGELRQPVWPAEVYGSISHCGATALAVVSRQ
                     PIGVDIEEIFSAQTATELTDNIITPAEHERLADCGLAFSLALTLAFSAKESAFKASEI
                     QTDAGFLDYQIISWNKQQVIIHRENEMFAVHWQIKEKIVITLCQHD"
BASE COUNT         6479 a         7659 c         7130 g         6054 t
ORIGIN      
        1 tctatttatt cacagcgcga atgaaaaata aaacactctg ctttgcagtg tttactttat
       61 aattaacaga cttatctcac tgatcaccct gtaacgttca gagagcgtct tcagatgtct
      121 atttataaaa ttcctcttcc gctcaatatt ctggaagctg cacgagagcg tattacctgg
      181 acactcaaca ccctaccccg cgtttgtgtc tcattttctg gcggcaaaga ctctggttta
      241 atgctgcatc tgacagccga acttgcccga cgaatgggca aaaaaatctg cgttttgttt
      301 atcgactggg aggcgcaatt ctcttgcacc attaactatg ttcagtccct gcgcgagttg
      361 tacaccgatg tcatcgaaga gttttactgg gttgcgctcc cgcttacgac gcaaaattcc
      421 ctttcacaat accaacccga atggcagtgc tgggaacctg atgtcgaatg ggtgcgtcag
      481 cccccgcaag atgcgataac cgatcctgac tttttctgct tttaccagcc tggcatgacc
      541 ttcgaacaat ttgtacgtga gtttgccgaa tggttttcac aaaaacgtcc ggcggcgatg
      601 atgatcggca tccgtgcgga tgagtcctac aaccgttttg tcgccatcgc cagtttaaat
      661 aaacaacgtt ttgccgacga taaaccatgg actacggccg caccaggcgg tcatagctgg
      721 tacatttacc ccatttacga ctggaaagtg gctgatatct ggacctggta tgcaaatcat
      781 caaagtctct gcaacccact gtataaccta atgtatcagg caggcgttcc tctgcgtcat
      841 atgcgaattt gcgaaccttt tggcccggag caacgacaag gattatggct ctatcacgtt
      901 atcgaaccgg atcgctgggc agctatgtgc gcacgagtca gcggggtaaa aagtggcggc
      961 atttacgccg gacatgacaa tcatttctat gggcatcgga aaatcctcaa gccagaacat
     1021 ttagactggc aagaatatgc attattgctg ctcaatagca tgccggaaaa aacagctgag
     1081 cattaccgca ataaaatcgc catttatttg cactggtatc agaaaaaagg catcgaggtt
     1141 ccacaaaccc agcaagggga cattggcgcg aaagatatcc cctcctggcg gcggatatgc
     1201 aaagttttac tcaataacga ttactggtgt cgagcattat catttagtcc tacgaaagcg
     1261 aagaactatc agcgttataa cgaacggata aaaggaaaac gtcaggaatg ggggatacta
     1321 tgcaacaacg attaacgcag gatttaacgc aatttctcgc cagcttgccg gaagatgatc
     1381 gcatcaaagc gattaatgaa atccgcatgg cgatccatca ggttagcccc tttcgtgaag
     1441 agccagtgga ttgcgttctg tgggttaaaa acagccaact tatgcctaat gattacaacc
     1501 caaacaacgt ggcaccaccc gaaaaaaact gctgaaaaaa tccattgaaa tagatggttt
     1561 tacccaaccc attgtggtca cgcatacgga taaaaatgcc ctggaaatcg tggatggttt
     1621 tcaccgccat gaaattggta aaggctcatc atcattaaaa ttacgcttaa aaggttattt
     1681 gccggtgacc tgtctggaag gtacgcgtaa tcaacgcatc gccgcgacta ttcgtcataa
     1741 tcgcgctcgt ggacgccatc aaatcaccgc catgtcggaa atcgtccgtg aacttagcca
     1801 gttgggatgg gacgataata aaattggcaa agagctagga atggacagcg acgaagtgct
     1861 gcgcctgaag caaattaacg gcctgcaaga gctgtttgcc gaccgtcaat attcccgcgc
     1921 ctggacggtt aaataactaa agctggcgca ggcgttcagc tgctgccagc aacgtcgatt
     1981 ccttcttggc aaaacagaga cgaatcagtt tatgtgggaa gggatcggcg caaaacaccg
     2041 acagcggaat cgccgctacg ccgtgctcct gcgtcagcca ctggcaaaac tcaacatcat
     2101 ccagggtaga aacctcgctg taatccacca gcaaaaagta tgtaccttca cacggtaaaa
     2161 tctccagccg gctttcattt aaagcattca ccagaatatc gcccttctgg cgataaaagt
     2221 ccggtaacgc aagataatgc tcaggttctg cacgtagcat atcagcaagc gccagctgtg
     2281 ccggggtatt caccgaaaag gtcagatact gatgtacctt gcgaatttcg gcgctgatgg
     2341 gcgctggcgc aacacaataa cccactttcc agccggtcat atgataggtc ttgccaaatg
     2401 aagaaaccgc cactgcccgc tcacgcagct gcggatgcgc cagcacactg gcatggccct
     2461 gttgtgaaaa gttgatgtgc tcgtagactt catcgctaat gacaaaaatc tcgtgcccgg
     2521 cgatcgcctg ccacaaagcg gcgaaatcag cctgctgcca gacagttgca ctggggttat
     2581 gcggagtgtt gaggatcacc agtctggtgc gctcgcttaa taatgcggca aattcctgcc
     2641 agtcaacgcg aaaatgcggt ggttgcagtg ccatacgctt cactattccc ccagaaagcg
     2701 cgatggcggg ggagtaactg tcatagctgg gatcaaaaca aatcacttca tcgccattgc
     2761 gcaccagtgc ggtaatcgcc gcgtataacg cttccgtcgc ccctgccgtt acggtgatat
     2821 cgctatcggc atctggttga tagccataca aacgttccgt tttctgagca atcgcctcgc
     2881 gcaaggcctg cacgccggtc atgggcgcgt attggtttgc cccctgggca acgtggtacg
     2941 ccagccgctc ctgtaaatag cgcggaccat caaaatcagg aaagccttgc gacaggttaa
     3001 tcgcctggtg ttgctgcgcc agcgcgctca tctgggtgaa aatagtggtg ccaagttgtg
     3061 gaagtttgct ttgtggaatc agagggttat ttgtcatttt tattgtacct gacgaagttg
     3121 cgtgttgaag gcactataac acgatgctag tatttggcaa tcaagacgtt tagatgtcta
     3181 aataaaacaa taaggacaac acaacatgcc tcacaatcct atccgcgtgg tcgtcggccc
     3241 ggctaactac ttttcacatc caggaagttt caatcacctg cacgattttt tcactgatga
     3301 acaactttct cgcgcggtgt ggatctacgg caaacgcgcc attgctgcgg cgcaaaccaa
     3361 acttccgcca gcgtttggac ttccaggggc aaagcatatt ttgtttcgtg gtcattgcag
     3421 cgaaagcgat gtacagcaac tggcggctga gtccggtgac gatcgcagcg tggtgattgg
     3481 cgtcggcggc ggcgcactgc tcgacaccgc gaaagccctc gcccgccgtc tcggtctgcc
     3541 gtttgttgcc gttccgacga tcgccgccac ctgcgccgcc tggacaccgc tctccgtctg
     3601 gtataacgat gccggacagg cgctgcatta tgagattttc gacgacgcca attttatggt
     3661 gctggtggaa ccggagatta tcctcaatgc accgcaacaa tatctgctgg cggggatcgg
     3721 tgacacgctg gcgaaatggt atgaagcggt ggtgctggct ccgcaaccag aaacgttgcc
     3781 gctaaccgtg cgactgggga tcaataatgc gcaggccatt cgcgacgtat tgttaaacag
     3841 tagcgaacag gcgctgagcg atcagcaaaa tcaacagtta acgcaatcat tttgcgatgt
     3901 ggtggatgct attattgctg gtggtgggat ggtcggtggt ctgggcgatc gttttacgcg
     3961 tgtggcggcg gctcatgccg tgcataacgg tctgactgtc ctgccgcaaa ccgagaaatt
     4021 tctccacggc accaaagtcg cttatggcat tctggtacaa agcgccttgc tgggtcagga
     4081 tgatgtgctg gcgcaattaa ccggggcgta tcagcgtttt catctgccga ccacgctggc
     4141 ggagctggaa gtggatatca ataatcatgc ggagatcgac aaagtgatta cccacaccct
     4201 gcgtccggtg gaatccattc attacctgcc ggtcacgctg acaccagata cgttacgtgc
     4261 agcgttcgaa aaagtggaat cgtttaaagc ctgacgtaca tatttagcag cagcgcgcgc
     4321 cgccttttcc accgtagcgc gcgtcctgcc gctcacggaa aaactcctcg taggtcatcg
     4381 gcgtttgatc gggatggtta acccgcatat gttcgacata gttgtcgtaa tcaggcatac
     4441 caatcatcag cttcgccgcc tgacctaaat attttccggc ttttgccagt gaatcaaaca
     4501 tcttggcctc cgctaaccat gttgctgaaa gagtgccgga tgcggcgtga acgccttatc
     4561 cggcctacaa atgctatcaa cgcgctcggt cagtcccctc gccccaccgg ggagagggtg
     4621 agggggaacc ctcggcgcca tgccaacatt cgccaacatt ccccctcact ctgactttag
     4681 tgtgcgcctt ttgcctgcgc cacgatctcc tcgacatttt ccggcattgg ctcatacggc
     4741 gtttctttcg ccgttggctt cggctctttc aatgccgcaa gtgccgtctt aatcgagaac
     4801 agtgccagaa ccaccacgac caccataaag aagatggtta acccggcatc cagacggttg
     4861 ttgaacacca gttgcgccag ctgtgactca gtatactgcg acggaatatt gccgctgtcg
     4921 atcattgcct ggaacttgtt agcaatggcc aggaagccga ctttcgcatc cgggctaaac
     4981 gctttctgcc agcctgcggt cagggtacaa atcagtagcc aggccgttgg taccagcgcc
     5041 acccaggcgt aacgttgacg tttcatcttg aacaacacca cggcacagag catcagcgcc
     5101 atccctgcca gcatctggtt ggcaatacca aacagcggcc acagagtgtt aataccgccc
     5161 agcggatcga ccacgccctg atggaggaag tagccccacg ccagtacgca cagcgccgtt
     5221 gccagcaggt tagcaggcag tgaatcggtc cgcttcaggc caggagacac cacgcccagc
     5281 agatcctgca acataaagcg cgcagcacgc gtacccgcat ctaccgccgt cagaataaac
     5341 agagcttcaa acagaatggc gaagtgatac cagaacgcca catccatcat accacccagc
     5401 gcaccgtgca ggatgtaagc catccccacc gccagcgtcg gcgcaccgcc cgcacgggaa
     5461 atgatcgact gttcacccac ttcgctggca atctggttga gcgtatctgg tgtaatgcta
     5521 aagccccagc tactcaccac ctgcgcggca gaagcgacta catccgccgt ccctgccgga
     5581 gccagcaccg ccattgggct gttcatggca aaatacacgc ccggatcgat gatacaggca
     5641 gaaaccagcg ccataatcgc cacgaaggat tccatcaaca tcccaccgta gccgataaag
     5701 cacgcctgcc cttcgttcgc cagcatcttc ggcgtggtgc cggaagagat cagcgcatgg
     5761 aagccagaca ccgcaccaca ggcgatggtg ataaacagga acgggaacag gttaccggtc
     5821 cataccgggc cagtgccatc gacaaatttg gtcagcgcag gcatggtcag cgtcgggcgc
     5881 ataatcaaaa tgcctaccgc cagaccgacg atagtcccga ttttcaggaa ggtcgagaga
     5941 tagtcacgcg gggccagcag caaccacacc ggcagcaccg ccgccacaaa accgtagccc
     6001 accagcatcc aggtcagctg cacgccggta aagtcaaagt acggtgccca ggtcggactt
     6061 tctgccaccc agccgccaga gataatggcg aaaatcagga acaccaggcc aatcactgac
     6121 acttcgccaa tgcggcccgg acgcagatag cgcaggtaga ttcccataaa aatcgccagc
     6181 ggaatggtga acgcaacggt atacgttccc cacgggctat gagtcagggc tttcaccacg
     6241 atcatcgcca gtactgcaag gataatgacc atgatcataa agcaggctac cagcgcaatc
     6301 accccggcag ttggccccat ctcttctttg accagctcac ccagcgatcg accgtcacgg
     6361 cgcgtagaaa caaacagcac catgaaatcc tgcaccgcac cagcgagaac caccccggcg
     6421 agcagccaga tcatccctgg caggtaaccc atttgcgccg ccagtaccgg cccaaccaac
     6481 ggacctgctc cggcaatggc cgcaaaatgg tgaccgaaca gcactttctt gtccgtcggc
     6541 acatagtcca gcccgtcgtt atggcgcacc gctggcgtca tacgcgtcgg gtcaaccgcc
     6601 agcacatttt tggcgatata cagcccataa aaacggtaag cgatcagata gatacagacc
     6661 gacgccacca caatccacag cgcgttgatc tgttccccac gatttaaagc aatgtatccc
     6721 agagcaaatg ctcccattac agagagcact gtccagacga ggtatttccc tgatttgttc
     6781 atagttgtta tccgtgtgcg tgtccataga gatgttacat tttgtttcta taacatcttt
     6841 gtacgattta acaacacggt aaccatgtaa atcggagttg aaaccaatat ttaacttaac
     6901 aatgttaact cgatcactcc gttaaccgag ttttcctgca atctcattta attctgtcgg
     6961 gctcatccca aaactgccgt acccagccga caagtgcagc aacgccgccc ctgttcatcg
     7021 aaaacgacga tttcccagct ctgattttgc cgcccaagat gcagcggctg gcagacgccg
     7081 cgtaccttgc cttcagacac cgggcgatga tgggtggcgt taagttccgt acccaccaca
     7141 cactgcccgt cacgggtcat cataaatccg gccatcgatc ccagcgtttc agccagcgcc
     7201 gccgacgcgc cgccatgcag caggccaaac ggctgatgag tacgggtatc aaccggcatt
     7261 tcggcttcca gcacatcatc tcccagacgg gtatacacaa ttcccagatg cgccaccatt
     7321 gtgttatcgc tggtggcgtt cagttcgtcg agcgttaaat ggcgtttcca gatcatgctt
     7381 atgcccccag cgttgagccg ccatcgacca caatatcctg tagggtgata tggctggcga
     7441 ggtcagaggc gaggaacaaa atcgtgttgg cgatctcttg tggacgggcg attttcccca
     7501 gcggaatgcc gagtttaaac tgctcgccaa agccgcgaat acgctgttct tcggcgtcat
     7561 cgctcaccca cagcgtgcgt tgcatatcgg tgtcggtgga gccaggcgaa accacattac
     7621 agcgcacgcc gctacccgcc agttccagcc cgacgctcaa caccaggctt ttcagcgccg
     7681 ctttcgatgc gccataagca ctcataccaa tacgcggcgt gtgtgcggcg tcggacgcca
     7741 cagtgacaat cgccccgccc cgctgacggc gaaactggtt catggtttgc tggaacaggt
     7801 taaacgcacc gccgacgtta accgcaaaag tctgctgcca gtcctcttta ctgagctgat
     7861 cggtcgcgcc catgcgcaaa attcccgccg cattgatcag cacgtccagt cgctccgttt
     7921 cagctaacag tcgctgacat acctgcgcca cctgcccggc gtcggcaaca tccattactt
     7981 cggtcgcaaa gggatattgc tcctgagtga acgcttgatc aaaacctgta actttcgctc
     8041 ccgcctcaac aaacgccagc gccgtggcgt agccgatacc tttacctgcg ccggttaccc
     8101 agacattttt accgctgaaa tccattattt cacctcgcgg gagagtagct tccaccaggc
     8161 gtcgatggtc gggtttttcg ccagcatgac aaagtcgata tcaccatgca ctttgcgcca
     8221 gcgcgccgcc agcgccatca tgcgcaccga atccagaccg tagtcgatca ggttgtcgtc
     8281 atcgaacggt tcatcggact cgtccagcaa cggcaggata acctcacgca gcgccgcttt
     8341 gctggcgggg acaggtgctg gcagtaattc ttcagtcatc accacccggc cagaccgccc
     8401 ggccacatat ttcagcgaca tcaaatgctc gtcacggctg aaatcggcca gcgcatccgc
     8461 caccataaac ggtttaatat cgcgcataaa tgcgtcggtt gcggtggtca tacagccaat
     8521 gtgggcatat accccggtaa taatcagctg gttacgtcca ctctctttga gcatttgctc
     8581 cagcggagaa cgatgaaacg cgctgtagcg ccacttcacc agcaccgtgt cgtcggcatc
     8641 tggcgtcagg cgatccacca ccttttgctg ttccggcgaa cgggtcaggc ccggccccca
     8701 catatcattc aacagcgccc gatcttcatc gctctgctct ttcggctggg cggtgtaata
     8761 aaccgggata ttgtgctgtt tgcagtagtc gcgcagcgca gcaatattcg cgatcacctg
     8821 ctccatcatc gggcagttct cgccccagaa gctgacaaaa tagtcctgca tatcatggat
     8881 taacaacgcg gcacgttgcg gttcaaaggc ccagtcgact ttattcttag ggatatcgtg
     8941 agactccggc agtgcgtaag cctgtaattt tggaatagcc atcgtgttct ctccttcagg
     9001 ctgatgcgcg tgacgccagc cactgacgta attgtttttt atcgactttc ccgaccgccg
     9061 tcagcggaag tgaatctaca cactccacgc gatccggtaa tttaaattcg gcaataccct
     9121 gttcacgcag gaaacgacgc acctgcaccg cgcgcagcgg ttctttcacc accagataag
     9181 cgcagctttt ttcgcccatc agctcatctt ccatgctcac cagtgcggcg tagatcaccg
     9241 ccgggtggcg cagcagcagg ttttcgatct cttcggcagc gatcttctca ccgccacggt
     9301 tgatctgatc tttctcgcgc ccctgcacgg tgatgtaccc ctctggatca atagagatca
     9361 gatcgccgga acagtaaaaa ccgttggcat caaaggcact ggcattgtgc tgcggacttt
     9421 tgtaataacc gcggaaggtg tacggcccgc gcgtcatcag gcgtccaact tccccttgcg
     9481 gcagtggatt tccttcagca tcagcaaccc atacttcatc gtccggacac attgggtaac
     9541 cctgggtatg gataattttc tccgcgctat catcaagtcg ggtgtagttc accagccctt
     9601 ccgccatgcc aaacacctgc tgcaacaggc agccaatctc agcgggaata cgcgccgcaa
     9661 gggtggctga aagacgtgcg ccgccgacct gtaacagttt cagcgaggca agctgcgccc
     9721 ggctttcacc ttcggtcagc gcctgcaacc acaggctgac tgccggtggc accagcgcgg
     9781 tgacgttcac ctgatgtttt tcaatcaatg ggaagcaaag cgtggcgctg ggatcggccg
     9841 ccagaacaac cgttcctccg gcaagaaaga cgcccagcga tcccggcgaa ctcatggcgt
     9901 agttatgagc cgccgggatc gcgcacaggt agcgtgtctg ttgtgtgaac tgacaaatct
     9961 cgacgctacg acgcacgctg tagtagtagt cgttatgagt gcgcgggatc agtttcggtg
    10021 tgccggtggt gccgccggaa agctggaaat aggccacttc atcagcaggt gatggcgtgg
    10081 cagtaaaatc ctcagccgga tggttaatcg catcctgcaa gttatgctca ccgctgtcgt
    10141 tgtgcagttg caccacgcga atggaggaat gttctgtgac gaaagtattg aggaaatcat
    10201 ccccgctaaa cagcgcatgt tggcgatcgg caatcagcaa tgcgggttca atctggctgg
    10261 cataggcgtt cagttcacta cgctgatggc tgaacagcgc cagcaccggc gcaacgccca
    10321 gtttcagcag cgcgaaaaag gtaatataca attcagagac gttacccagt tgtaccagcg
    10381 cggtttcacc aggtttaatg ccctgacggc gtaaactaca cgcgaggtta tccgccgcct
    10441 gattcagctc ccggtaactc aactgtcgct cgccgtcgat aaccgcgatg ctgtcactcg
    10501 cagcgtggcg agtcagaatg tcggtcagcg gcaaatcctg ccagtagcct ttttcccgat
    10561 agcgacgggc aaactcttcc ggccagcggg tgaatggaat gctcatcctc gctccttaat
    10621 gcaatccaaa aacgttcaac atggtagaaa gtttgacgcc tgtttcgcgc cactcaccca
    10681 acggtgacga cgcaggcaca atccccgctc cggcaaacag acgcacctga ttttcccgca
    10741 gcttcgcgca gcggatggtc accacccatt cgccgttacc ttcgctgtca caccaaccca
    10801 caatgccgcc aaacagttcg cggtcgaatg gctccagttc agcaataacc tgggtcgcgg
    10861 cctgatgcgg gaaaccgctc agcgcggggg ttggatgcag cagacaggcc aaagtcagtg
    10921 cgttttcttg cgaattcgct ttaccttcaa agggagttgc gagatgccac agcgtcggcg
    10981 tggtaatcaa ttgtggagag gagggaacgt gtaactcact actgcgttcg cgcagtacct
    11041 ctttcatcgc ctgagtcacc agttcatgtt catggcgatc tttttctgac gccagcagac
    11101 gattacccgc ttcgcgatcg agcacttcat ccggctgacg acgcgcggaa ccggctaacg
    11161 gaatggagct aaaacgctcg ccgtctttac gtagcagcag ttccgggctg gcccccagca
    11221 ggacgccacc atcagccagc gggacatgga agttgtaact aaccgggttt tgcgcaatca
    11281 accgttccag caatacgcca ctatcaatgg cggcgtcagt ggtgatatca atcaatcgtg
    11341 acaacaccac tttgtcgacc tgcggcgtgg cggtaagtgc ggcggcgcga gcaaccatct
    11401 gttcaaacgt ggtttgttca ggaattgcct ggcgttccac cacgttcagc gactggctgc
    11461 gggtaaaacg gcgggctgag gtctgttttt cctggcggga gaacgactgc caggattcgg
    11521 gaatatacag cgacgaaggc tgacgtggat cgaagggaat cgccccgacc atcaccggat
    11581 ttttgatgcc ctgcgctttg gcatcggcaa acagcgcggc gagtttttgc tggaagggac
    11641 tgtcgggcga atccccgttc acagccggtt catcgaagcg ggcgaaacat cctgacgtcg
    11701 taaaactgcg gtacggcgac ataaagaaaa agcgattggg cgcaagtgtt gccatggtct
    11761 gctgtacttc ctcagccagt gacgtatcca tatcatcctc cacaaaatga taaaggcttt
    11821 aataatgatt atcatttata ttttcggtcg ctaacctaaa agcaaacgcg cattatgtca
    11881 actcttgagg taacgcaatc tgcaactcgg ggttgcatct ggttgtggtt acaatgaaaa
    11941 tgagaagcat tattgatgga ttcgcataag cgcaatgtga tgtcctgcgc cgttctgccc
    12001 cctctccctt ccagggtgag ggctggggtg agggttaatg ttcgcaccag tgctggctgt
    12061 tcccctcacc ctaaccctct ccccaaaggg gcgaggggac ggattgtgcg ctttgtcgaa
    12121 tttgtcatta cgcccttaac cttattaata acaggaagct gatttgtgag actcgccccg
    12181 ctctaccgca acgcccttct attaacagga cttttgcttt caggaatagc cgcagctcag
    12241 gccgctgact ggccgcgtca gattactgac agccgtggca cacatacact ggaaagccag
    12301 ccgcagcgta ttgtttccac cagcgtcacc ctgaccggct cactgctggc gattgatgct
    12361 ccggtgatcg ccagcggcgc gaccacgccg aataaccgcg tcgcggatga ccagggcttt
    12421 ttacgccagt ggagcaaggt ggcgaaagaa cgcaaactgc aacggctcta tatcggcgaa
    12481 ccgagcgccg aagccgttgc cgcgcaaatg ccggatctga ttttaattag cgcaaccggc
    12541 ggggattcgg cgctggcact gtatgatcag ctttccacca tcgccccgac attaatcatc
    12601 aattacgacg acaaaagctg gcagtcgctg ttaacgcagc ttggcgaaat taccgggcat
    12661 gagaaacaag cggcagagcg gattgcacag tttgataagc aactggcagc ggcgaaagag
    12721 caaatcaaat taccgccgca gccggtcact gccattgtct ataccgccgc tgcacacagt
    12781 gccaatctct ggacgccgga atcagcacaa gggcagatgc tggaacaact cggctttacg
    12841 ctggcgaagt tgcccgcagg gttaaacgcc agccaaagcc agggcaaacg ccatgacatc
    12901 attcagcttg gtggggaaaa tctggctgct gggttaaatg gcgagtcact attccttttc
    12961 gccggtgatc agaaagatgc cgatgcgatt tatgccaatc cactgctcgc gcacctgccc
    13021 gcagtacaaa acaagcaggt ttatgcgctg ggaaccgaga cgttccgcct ggattactac
    13081 agcgccatgc aagtgctgga acgacttaag gcgctgtttt aagcattaac tgtcagacgc
    13141 tgtcacctgc ggcggcgtct ggcgaaaacg tcgcaactcc accagcacca gcaataacaa
    13201 cacgccgata atcaacaaac caaaaccgct cgcgcttgcg gaagcaaccg gtgtcatcat
    13261 cgcgcccaaa ccacccagca gcgccgcgcc tatggcatcg cccgtcacgt tctgcgccgt
    13321 ccacaaaccg ttaatccgcc ctaacatcgc ttccggggtt tgcgtttgca gcattgtata
    13381 ttgcagcaac gaactaaccg cgctcagcca gccgaacagc gccagacaaa ccacgcctaa
    13441 aatccacatc ggcatcaggc caaacagacc aatggcgagg aacgatccca gcgtggagag
    13501 cagcatcaat aaccctggtc gcgcactatg tgccagcttc ccgctggtta acgcaccaat
    13561 agccgcgccg agcgggatcg ccgcgtagag aaaaccaatc tgtgccgctg acatctgcca
    13621 gttgtcagcc agcgccggat acagtacccg caccgcgctc gccatcgtca ataaaccacc
    13681 cagcagcgca atccctccta ccagcgggct ggcgagcaga aaacgaaatc ctgccagtaa
    13741 tgatttcaac ggatgctcgc gcggctgcgg tggcggtggc aacgccggaa ggcttaacaa
    13801 cggtagcaag gtaataaacg tgcccgccgc cgccagcccg tagttccagg ctacgccgcc
    13861 ggtcgccagc aataaaccgc caatcatggg cgaaatcacc gaccccagac gcacggtcaa
    13921 catggtgatc gccccggcct gcattaagtt ttcacgccct accagtgcag gtgttgccgc
    13981 cagtagcgcc gtaacaccaa gtgatgcgaa aaaaccatcc cataaaccaa gtaaatagat
    14041 tgccagcaat gacggctccg gcagcagcgc gttcaggcac agtccaatga agccaatgcc
    14101 acaggtgccg cgcgccagca aaatcacttt tttgcgttca tagcgatccg ccagcacacc
    14161 gccaaccatc aggccaacaa acatcgcgcc gccggtcagc gtcaccgaaa gccccacctg
    14221 ccaggtagaa tgcgtcatca tctggatctg caccggcacc gcgacgccga gcaaacccag
    14281 agacacaatt gagataaaac gagcgaggaa tactgcgcga aacgccgggt gcgttttcaa
    14341 caggctgagg ttaagcagcc aggattgttt attcattaca atgccttgcc atcaaatatt
    14401 ttaaatacct attcttaggc tgcacatgct aacatatcca aataagatcg ataacgataa
    14461 ttaatttcat tatcatggaa gttcgtatgt ctggttctgt tgccgtgaca cgcgccattg
    14521 ccgtgcccgg attgctgtta ttactgatta tcgcgacggc attaagcctg ctcattgggg
    14581 caaaatcact ccccgcttcc gtagtgctgg aggccctctc cggcacctgc cagagcgccg
    14641 actgcaccat cgtgctcgac gcgcgactgc cgcgcaccct tgccggttta cttgcaggcg
    14701 gcgcgcttgg ccttgccggg gcgttgatgc aaaccctcac ccgaaatcca cttgccgacc
    14761 ccggcttgct tggcgtgaat gccggagcca gctttgccat tgtgctgggt gcggcgctgt
    14821 ttggttactc atccgcgcag gaacaactgg cgatggcctt cgccggggcg ctggtggcct
    14881 cattgattgt tgcctttacc ggcagtcagg gcggcgggca gttaagtccg gtgcgtttaa
    14941 ccctggcggg cgtggcgctg gcggcggtgc tggaaggact gaccagcggc atcgccctgc
    15001 ttaatcctga cgtctacgat caactgcgtt tctggcaagc cggttcgctg gatattcgca
    15061 atctacatac cttaaaagtg gtgctgatcc cggttctgat cgccggagca actgcgctat
    15121 tactgagtcg cgcgctgaac agtttgagcc tcggcagtga caccgcgacg gcgctgggca
    15181 gtcgcgtggc gcgcacacag ttaattggtc tgctggcgat taccgtgctt tgtggtagtg
    15241 cgacagcggt agttggcccg attgccttta ttggcctgat gatgccgcat atggcgcgtt
    15301 ggctggtggg tgccgatcat cgctggtcgc tgcccgtcac gctactcgcc acccctgccc
    15361 tgctgctgtt tgccgatatc atcgggcggg tgattgtgcc cggcgagttg cgcgtttctg
    15421 tggtcagtgc gtttattggt gcaccggtgc tgatcttcct cgtgcgacgt aaaacgcgag
    15481 gtggtgcatg atttacgtct ctcgccgatt actcatcacc tgtttgctgc ttatttccgc
    15541 ctgtgtggtt gcaggtatct ggggattacg cagcggtgcc gtcacgctgg aaacctcgca
    15601 ggtattcgcc gcgctgatgg gcgatgcgcc gcgcagtatg acgatggtgg tcaccgaatg
    15661 gcgtttacca cgcgtgctga tggcgctgtt gattggcgcg gcactgggcg tcagcggtgc
    15721 gatttttcag tcgctgatgc gtaacccgct cggcagccct gacgtaatgg gctttaacac
    15781 cggggcgtgg agcggcgtgc tggtggcgat ggtgctgttt ggtcaggacc tgacggctat
    15841 cgcgctggca gcaatggtgg gcggcattgt cacttctctg ttggtctggc tgctcgcctg
    15901 gcgtaacggt atcgacacct ttcggttgat tattatcggc atcggcgttc gcgccatgct
    15961 ggtggccttt aatacctggc tgttgctgaa agcgtcttta gaaacggcgc taacagcggg
    16021 tttgtggaat gccggatcgc tcaatggcct gacgtgggca aaaacttcgc cttccgcacc
    16081 catcattata ttgatgctca ttgccgccgc cttgctggtg cggcaaatgc gcttgctgga
    16141 gatgggcgat gataccgcct gcgcgctggg cgtcagcgtc gaacgttcgc gtctgttaat
    16201 gatgctggtt gcagtggtgc ttaccgctgc ggcaacagcg cttgccgggc cgatttcctt
    16261 tattgcttta gtcgcgccgc acatttcccg acgcattagc ggcaccgctc gctgggggct
    16321 aacccaggcg gcgctatgcg gggcgctgtt actgctggcg gccgatctct gcgcccaaca
    16381 actgtttatg ccgtatcaac ttccggttgg cgtcgttacc gtcagcctcg gcggtattta
    16441 ccttatcgtc ttgttaattc aggagtctcg caaaaaatga ccgaatcagt agcccgtttg
    16501 cgcggcgaac agttaaccct gggatatggc aaatataccg ttgcggaaaa tctgactgta
    16561 gaaattcctg atggtcactt cacggcaatt atcggaccaa atggctgtgg taaatccacg
    16621 ttactgcgta ccttaagccg cctgatgacg cctgctcacg ggcatgtctg gctggatggc
    16681 gagcacattc aacattacgc cagtaaagaa gttgcacgcc ggattggtct gttggcgcaa
    16741 aatgctacca cgccgggcga tatcaccgtg caggagctgg tggcgcgtgg acgttatccg
    16801 catcaaccgc tgtttacccg ctggcgcaaa gaggatgaag aagcggtaac gaaagcgatg
    16861 caggccacgg gaataactca tctggcagat caaagcgtgg ataccctttc tggcggacaa
    16921 cgccagcgag cgtggatcgc gatggtgctg gcccaggaaa cggcaattat gctgctcgac
    16981 gaacccacga cctggctgga tatcagtcat cagattgatc tactggaact gttaagcgaa
    17041 ctgaaccgcg agaaaggcta taccctggcg gcggtgctgc acgatcttaa tcaggcctgt
    17101 cgttacgcca gccatttgat tgcattgcgg gaagggaaaa ttgttgctca gggagcgccg
    17161 aaggagattg tcactgctga actgattgag cgcatttatg gtctgcgatg catgatcatt
    17221 gacgatccgg tagccggaac gccgcttgtg gtgccgctcg gacgaacggc accttcaacc
    17281 gcaaatagtt aaactaagtg gtctgccatc atggcatcct gttttctgga tgccatcgca
    17341 tagcgcaata acacgctacc acaagccacc atcccgccga tcagcgcgga aaggatcaca
    17401 ataatcgcct tacccggacc gtcttttttc actggcaatg acgggcttaa ctgatattta
    17461 aacggcgtaa aattcacatc gttgatattt gcttttgtta actgctcaac aagatactga
    17521 cgattacgta attcaccgtt cagttccgca acgtcagtga ccgctttttc aatttccagt
    17581 ttgcgttcaa taccgtctgc gccgagagaa atagagaaat cggggtcatc tttaacggcc
    17641 tgaccattac tgtatacggg ctttttaatt cccgccgcgt tggcaatgtc gagtgaataa
    17701 ttgaggcgct gaatgtttgc atcaagttga tttttcgttt taatgcgatc ctgagccagt
    17761 ttttcttttt caaactgggt tttgatctcc agtttattac ggacgttttc tatcgactct
    17821 ttcaccacca acgcagagat ataatcgata tacccgctca aaacggtctg cgcctcttta
    17881 ctggttggcg cggtaaaact tagcgtccag gaggtataca gtgacggttc atcttttttc
    17941 ttactggcat tgtcatcaac cgctttcatt ttttcgctca atgcaacaat agcgcgatgc
    18001 aaatccagtt catcgatttt cgcctctttt aattggtcca tcacataagg tgatgaacgc
    18061 aggtactctt ccagcaagct aacagactga aacttcttga taaacaggtt aaatgcaccg
    18121 ccacggtcga tattaacatc gagatcaaga acgcggagtt tggtaaaggt tttctccagg
    18181 tcctgccact gaatggcttc ggcgggagta ataacggctg agctggtcca tttttgcggc
    18241 aggatgaaag agatcagtaa acctgcgcag gcaaatgcaa aaacgaccgc catgaccgtt
    18301 tttttggccc gccataaaac ttcgattaga ttaagtaaat caatttcatt attactgggc
    18361 gacgccagag gatactcaga aaaatgagcg tcacttccct gtttaatatt caatgatgac
    18421 atggggtata acctgaaaat gagtccaata ggggaaaatc tccacggcgc tcactttacc
    18481 agttggtgag aattatccga agctgaagtt tgtaaattcc ttccactgaa tgcaacattc
    18541 actctattta agagaaataa ctagcaaact tataaactaa ataaatgatt aacccataat
    18601 tatttcctat aattatgggt taatagataa tgttaattta cctgtttagc gttgcgcgaa
    18661 taatcggccc aattttttca aacgcctctg aagatataat atccacatgc gcacaatcct
    18721 gacgataaat atccagctcg gctatccacg gcgaccaggc gcgttcggga ctcatacctt
    18781 cctgaagcgt gcgttcagca acaaacagcg tcgctttacc gtcaaacggt acgctatgag
    18841 cagtcgtcag caggcgcact gcatcagcgt agttgccttc aatggtggta aacaactccg
    18901 ttgaagtacc tccctgctgt gccgccagga aggcctcgcg ttcgcggtta atctccgcca
    18961 gcacttccgg gtccagacca ttagcttctt tttcctgcca gttttgcgtt tctggcggcc
    19021 aggtatccag caagccaaga aatgccacct gttcgccacg ggcacgcagt cgcgccgcaa
    19081 taccctgcac cagcgtaccg ccaagggaat accccagcag gtaataaggg ccgtgcggtt
    19141 gttgctcaag taacgttgcc agatgcgctt cgcagacttc atccaggttt gtcgctgtct
    19201 gcatgggtcc atgagggcgc ggcgactgaa tgccaataat cgaccattgt ggatcgagat
    19261 aacgcgagag cacgctaaac tgccaggcaa aaccggatgc cggatggaaa caaaacagcg
    19321 tcgggccatt accttcacgc aacggcagaa tggtttcgaa tcccatgcgc cgggagctgt
    19381 cctcttcacc atcaataatc gttgccagtt tggcgacggt tgacgcgacc atcacctgcc
    19441 ccggcgtcac ctgacgagca aactgtcgac ttaactgcgc tgccagtttc attgccagta
    19501 gcgaatgacc gccaagcgca aagaaatcag catcggcatc ctgcacgtca caacccagca
    19561 acgacgcgaa tgccgcggcg ataatcgttt cactgcccgc tttcggtgca cgtcccgacg
    19621 cttgtgtctt cagttcaggc aacggtaagg ctttgcgatc cagcttgccg ttggcgctaa
    19681 gaggtaactg tggaagttgc agcagaacca ccggcaccat atgcggcggc aatgtttcgc
    19741 gaagctgtgc ctgtaatgcg ctggtatcca acggcagacc tgattgcgac accagatagc
    19801 ccaccaactg acgcgcatca ccaccggtgg ctgccgccag gttaatcaca caggcgtggg
    19861 taacggcttg tttgacatcc ggcagcgcct gcatcacgcg atcgatttcg cccagttcga
    19921 tacgctgccc gcgaattttt agctgatcgt cactgcgccc gaggtactcc accgcgccgt
    19981 tatccagcca gcgggcgacg tctccggtac gatacatccg ttcacctggg gcaaaaggat
    20041 cggcaataaa acggctggcg gtcagatcgg gtcgtccaag atacccctgc gccagttgga
    20101 tgccggtgag atagagatca cccgccacac ccggcggcac cggatgcatc atcgcatcaa
    20161 gaatacgcag acccgtattc cataccggat aaccaatcgg cacactgctg ccgcgcacct
    20221 gtgccagttc ctcgccaaaa gccggatacc agctgacatc taccgccgct tccgtcgggc
    20281 catacaggtt atgcaacggc gcgccggtta actgttgcca ttcgcggcat aaatcagccg
    20341 gtaaggcctc accactacag aaaacctgtt tcaacgtcgc gcaattctgg cgagcggttt
    20401 gcggcgtcag cgatgcaaca aatgccgcca gcatcgacgg cacaaagtgc gtggtcgtta
    20461 cgccatattc ggcaaagaat tgctgcatag cgagcgggtc acggtgcgct tccggttcag
    20521 ccatcaccag ttttgcccct gcgataaacg gccagaaaaa ctcccacacc gagacatcaa
    20581 aactgcacgg cgttttttgg gcaacgacat cttcgcctgt aagcgggtag tgattttgca
    20641 tccataacag gcggttaacg atagccgtct gcccgaccat tacccctttc ggcctgccgg
    20701 tggagccaga ggtaaagatg atataagccg tgtggtgcgg ttgtgaaagt tgcagcggcg
    20761 cactgccctg gggggtaagc ggggcgttat agcaaaggct tgttaaattg ggaacatcgc
    20821 taaagcgcgg cagttgatcg tcggtggtaa ttaacagcga cggacgcgca tcttccagca
    20881 tcattttcag gcgatcgtcc ggatagccgg tatccagcgg tagccaggcc gcgccagctt
    20941 caactattgc atggagtgcc agggtcaaaa agaccgagcg cggtagtgcc accgccacgc
    21001 tgtcccctgg tttaacgccg cgctcacgca gcagattcgc cagcgccacc acctgctcgc
    21061 gcatttcccg atagctgaac aggtaacgcg catctgccag cgccggagca tccggtgttt
    21121 ttgccgcttg ttctgccacc agcgcgctca gcgtggtttc tggaatctca acctgagtgg
    21181 cgttgatctg cgccagctgc gcatactcac ctggcagcat aatatcgaca tcgccgcaca
    21241 acagcgccgg atccgcggcg aactgggcaa tcagcatttt caggcgttca gcatgctgga
    21301 ttaacgttgg ctcatcgtaa cgctgtttat tggcgaggat ctcaatactc aaatcaccgt
    21361 gttcatccgg gaacagggcc agttcaaggt cattaaccgg accggttgcc agggtatggg
    21421 tttgcgtctg aacaccagga atatccagtt ggtaatcaaa taccttgata ttgagtaccg
    21481 gaccgaacag cggttcctct cctgccgctc gcccgctgtc acggacaatt tgttcggcat
    21541 cgtaacgttg atgacgacgc atttttttca gttgtgctgc cagtcgggtt gccagttccg
    21601 gcagcgtttc ttgcgccgca atgtgaatac ccaacggtaa aacgttgagc acgggtccgc
    21661 tagccgtcag cgccgccgag cccagtcgac gcataaagat aaatccggcg gcatagtcca
    21721 tgcgattgca caatcgcccc agccacaagg ctgccagcgc aagggctaaa tcggtacgct
    21781 gcacacctga aagttgcgta gccagctggc ggaattcccc gtcggtaaat tccagtttca
    21841 ggcgcagaat atctgccgaa gcgctgcgcc ccgctaaagg tgccggagaa agtgacgcgg
    21901 gcggcggcag ttgacgacgc tgttctgccc agaatgccgc atcacgctgc caggcttcgc
    21961 tttcgcggta ttgctggtac tcttccacta catcagcgaa aggcgtaaac ggcgaagcag
    22021 gcgttggttc gccacgcagc catgtgcagt aaatattggc gatctggcga gtaatggccg
    22081 ggaaactgaa gccatcgacc agcaaatggt gataacgctg ataccagtac cagcggttat
    22141 ccgcaacatg tatcagctga tgaaagacca gtggtttacc gctatcgacg cgcaaatctt
    22201 gttgcaaatc cgccagcatt aatgcctgcg cagtaccgtg cggatcaata ttggttcgca
    22261 ggtcgataat ttctggcagt tcgaatatca gcgcatcatc gacccactgc cagacttcgc
    22321 cgttatcttc cgtaaaacgc atccgcaacg tatctgcttg cgctagtccg acaaccaccg
    22381 cgcgggccag taatggcgca tcaacatctc cagttaactc aacgtaatgc gccacgctcc
    22441 aggcggaggg taaatccgac agtttttctg ccatccagat gccgggctgt gcggcgacca
    22501 aaggtaaatg ctggctcatt gtgcctcctg caactgggtg aaattggccg gtgtcagagt
    22561 acgccagtgg acttccagcc actgctggca ggacgcctgt gactgcggct gacacacaat
    22621 gtcccagcct gccggtaagg cgcattgttg cggccacaga ctgaattgcc cctgcgcatt
    22681 gcgcaaaatg taaaacgctc cctgtggatc atcgaaggga ttactgaatg ccatattcaa
    22741 ctcctgtcat ggaaaagtgg ttgccagagg tcgattagcc cctgcatcaa gccaccgcgc
    22801 caacaaagcg catcatgtcc gccgtcaacc tgacgccaga aaatggattc ttttatgggg
    22861 tgtaattgcg catacagcgc ctgattggcc cgcataatca tcggctcgcg aatacccgct
    22921 tccagcacaa tgcgcagacc ttcggcgcta acttcaccag ctttaagctt ttcaagtaac
    22981 acgccctctt gctgcccgcc ccgatgcggc caccagtacg atcctgactg gcttaataca
    23041 cagccaaagc gttcaggcca gtgcagtccg gcatacagcg cggacagccc accaaaactc
    23101 tgcccggcga ccacggtgcg atcggcgcga tcgctaaaag gggcaatagc tttcaccagg
    23161 ggtaataact cttgctgtac tgccagccag aaatccgcat tacacggcag ttcgtgggcg
    23221 cggtgcgtgg tgtcgatagc gtcgatcaac acatacacgg cgggaggaag ttgctgacga
    23281 tgggtcagcg aagtcagcac aggccagacg ggcatacttt gcgcccaaaa ttcgccatcg
    23341 agcaaaactg ccagcggacg ttcttctgct gttgcatcgc cggtggtaaa aatccataca
    23401 cgacgtgaat ttttcaaccg ttcacttttc cagataattt ctttggctgc tatttctggc
    23461 gcttgcggac aatcccatcc cggttgcaga ggcgcttgcg gcatttcgag tgcagaaaca
    23521 gcgtgccctc gcccgccttt ccagctttgt ggattcagcg gatcggctat tgcctggggt
    23581 aatagttttc gccagccttc acgtaattcg aggcgatcgg gggatggtgc agaaaaaatg
    23641 tcatcgcgtt cggtgggaat aaagcagtag ctgccgcgcc agttggcatt gagttgtgtc
    23701 gtccactgcc agacatcagt acctgcaatt cgctgcatcg actggggctg gctgttctga
    23761 tgatgatcgg tcacaccagt gatgtagatc catacgcgct ttatcgtcga gtattcttca
    23821 gaaccttggg gatcacgcca ccagaaagtg acctcaaaca tttcgtcatt cagacgctgc
    23881 cattccgggc catgtttcga ctgccaccag ctctcacttc ctacttttaa cgccgtcaca
    23941 ccataacccc atgtttactg tgcaattttt cattgattgc agaaatatat tgataatatt
    24001 attgataact atttgcattt gcaatagcgt aatggcgcgc cgtgggaagc gcggacattt
    24061 attaaccaac tgcactgcgt gtctttcagg atcaaaggtt ttcgcggtag cgggatgcgt
    24121 cgtgttgagg acgaccatgc ccgacagttg caattcgtgg caaaaatgca ggaataaaac
    24181 aatgaacaag aagattcatt ccctggcctt gttggtcaat ctggggattt atggggtagc
    24241 gcaggcacaa gagccgaccg atactcctgt ttcacatgac gataccattg tcgttaccgc
    24301 cgccgagcag aacttacagg cgcctggcgt ttcgaccatc accgcagatg aaatccgcaa
    24361 aaaccccgtt gcccgcgatg tgtcggagat catccgtacc atgccaggcg ttaacctgac
    24421 cggtaactcc accagtggtc agcgtgggaa taaccgacag attgatattc gcggtatggg
    24481 tccggaaaac acgctgattt tgattgacgg caagccggta agcagccgta actcggtgcg
    24541 tcagggctgg cgtggcgagc gcgatacccg tggtgatacc tcctgggtgc cacctgaaat
    24601 gattgaacgt attgaagttc tgcgtggtcc ggcagctgcg cgttatggca acggcgcggc
    24661 gggcggcgtg gttaacatca ttaccaaaaa aggcagcggt gagtggcacg gctcctggga
    24721 cgcttatttc aatgcaccag aacataaaga ggaaggtgcc actaaacgca ccaacttcag
    24781 cctgaccggt ccgctgggcg acgaattcag cttccgcttg tatggcaacc tcgacaaaac
    24841 ccaggctgac gcgtgggata tcaaccaggg ccatcagtcc gcgcgtgccg gaacgtatgc
    24901 cacgacgtta ccagccgggc gcgaaggggt gatcaataaa gatattaacg gcgtggtgcg
    24961 ctgggacttc gcaccattgc aatcgctgga actggaagcg ggttacagcc gccagggtaa
    25021 cctgtatgcg ggcgataccc agaacaccaa ctctgacgct tacactcgct cgaaatatgg
    25081 cgatgaaacc aaccgcctgt atcgccagaa ctacgcgctg acctggaacg gtggctggga
    25141 taacggcgtg accaccagca actgggtgca gtatgaacac acccgtaact cgcgtattcc
    25201 ggaaggtctg gcgggcggta ccgaagggaa atttaacgaa aaagcgacac aggatttcgt
    25261 cgatatcgat ctcgatgacg tgatgctgca cagcgaagtt aacctgccga ttgatttcct
    25321 cgttaaccag acgctgacgc tgggtacgga gtggaatcag caacggatga aggacttgag
    25381 ctccaatacc caggcgctga ccggggcgaa caccggcggc gctattgatg gtgtgagtgc
    25441 caccgaccgt agcccgtatt caaaagcaga aattttctcg ctgtttgccg aaaacaatat
    25501 ggagctgact gacagcacca tcgtaacgcc ggggctgcgt ttcgatcatc acagtattgt
    25561 cggcaataac tggagcccgg cgctgaacat atcgcaaggt ttaggcgatg acttcacgct
    25621 caaaatgggc attgcccgtg cttataaagc accgagcctg taccagacta acccgaacta
    25681 cattctctac agtaaaggtc agggctgcta tgccagcgcg ggcggctgct atctgcaagg
    25741 taacgatgac ctgaaagcag aaaccagcat caacaaagag attggtctgg agttcaaacg
    25801 cgacggctgg ctggcgggcg tcacctggtt ccgtaacgat tatcgcaata agattgaagc
    25861 gggctatgtg gctgtagggc aaaacgcagt cggcaccgat ctctatcagt gggataacgt
    25921 gccgaaagca gtggttgaag gtctggaagg atcgttaaac gtaccagtta gcgaaacggt
    25981 gatgtggacc aataacatca cttatatgct gaagagtgaa aacaaaacca cgggcgaccg
    26041 tttgtcgatc atcccggagt acacgttgaa ctcaacgctg agctggcagg cgcgggaaga
    26101 tttgtcgatg caaacgacct tcacctggta cggtaagcag cagccgaaaa aatacaacta
    26161 caaaggtcag ccagcggttg gcccggaaac caaagaaatc agtccttaca gcattgtggg
    26221 tctgagcgcg acctgggatg tgacgaagaa tgtcagtctg accggcggcg tggacaacct
    26281 gttcgacaaa cgtttgtggc gtgcgggtaa tgcccagacc acgggcgatc tggcaggggc
    26341 caactatatc gcgggtgccg gggcgtatac ctataacgag ccgggacgta cgtggtacat
    26401 gagcgtaaac actcacttct gatgctaacg tcagattgtt gacaaagtgc gcgtcgttca
    26461 tgccggatgc ggcgtgaacg ccttatccgg cctacaaaaa tcttgccaat tcaatatatt
    26521 gcaggaccat gtaggcctga taagcgtagc gcatcaggca gtttagcgtt tgtcatcagt
    26581 ctctaatatg gtcgacatga aaactacgca tacctccctc ccctttgccg gacatacgct
    26641 gcattttgtt gagttcgatc cggcgaattt ttgtgagcag gatttactct ggctgccgca
    26701 ttacgcgcaa ctgcaacacg ctggacggaa gcgcaaagcc gaacatttag ccgggcggat
    26761 cgctgcaatt tatgcgctgc gggaatatgg ctataaatgt gtgcccgcaa tcggcgagct
    26821 acgccaaccg gtgtggcctg cggaggtata cggcagtatt agccactgcg gggctacggc
    26881 attggccgtg gtttcccgtc aaccgattgg cgttgatatc gaagaaattt tttctgcgca
    26941 aacagcgacc gaattgacag acaacattat tacaccagca gaacacgagc gactcgcaga
    27001 ctgcggttta gccttttctc tggcgctgac actggcattt tccgccaaag agagcgcatt
    27061 taaggcaagt gagatccaaa ctgatgcagg ttttctggac tatcagataa ttagctggaa
    27121 taaacagcag gtcatcattc atcgtgagaa tgagatgttt gctgtgcact ggcagataaa
    27181 agaaaaaata gtcataactc tgtgccaaca cgattaattg acaacatctg gtacgattcg
    27241 cccgcagcca tcactgaccg ctggcgaaag tgtaaagcag gtgccttacc actctgacct
    27301 gacaaccgga tatgcgggga tt
//
LOCUS       DAZFQD010000061        27027 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.061, whole genome shotgun sequence.
ACCESSION   DAZFQD010000061 DAZFQD010000000
VERSION     DAZFQD010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27027
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.061"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            8..772
                     /gene="ycaL"
                     /locus_tag="ACHKTW_002618"
     CDS             8..772
                     /gene="ycaL"
                     /locus_tag="ACHKTW_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HFO6463619.1"
                     /translation="MKNTKLLLAIATSAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
     gene            945..1628
                     /gene="cmk"
                     /locus_tag="ACHKTW_002619"
     CDS             945..1628
                     /gene="cmk"
                     /locus_tag="ACHKTW_002619"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFO6463620.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQVIEKALQ
                     YARQKLALA"
     gene            1739..3412
                     /gene="rpsA"
                     /locus_tag="ACHKTW_002620"
     CDS             1739..3412
                     /gene="rpsA"
                     /locus_tag="ACHKTW_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFO6463621.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            3572..3856
                     /gene="ihfB"
                     /locus_tag="ACHKTW_002621"
     CDS             3572..3856
                     /gene="ihfB"
                     /locus_tag="ACHKTW_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA];
                     GO_process: GO:0075713 - establishment of integrated
                     proviral latency [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HFO6463622.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            4063..6326
                     /gene="ycaI"
                     /locus_tag="ACHKTW_002622"
                     /pseudo
     CDS             4063..6326
                     /gene="ycaI"
                     /locus_tag="ACHKTW_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309023.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
     gene            6363..8111
                     /gene="msbA"
                     /locus_tag="ACHKTW_002623"
     CDS             6363..8111
                     /gene="msbA"
                     /locus_tag="ACHKTW_002623"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0034040 - ATPase-coupled lipid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HFO6463623.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DSLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            8108..9094
                     /gene="lpxK"
                     /locus_tag="ACHKTW_002624"
     CDS             8108..9094
                     /gene="lpxK"
                     /locus_tag="ACHKTW_002624"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HFO6463624.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     STGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLTQLTSLASGN"
     gene            9131..10363
                     /gene="ycaQ"
                     /locus_tag="ACHKTW_002625"
     CDS             9131..10363
                     /gene="ycaQ"
                     /locus_tag="ACHKTW_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ"
                     /protein_id="HFO6463625.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRQASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTMLQKGLRQAITDFASWQQATRVTLGRC
                     PQGLFTDCRTGWEIDPVA"
     gene            10415..10597
                     /gene="ycaR"
                     /locus_tag="ACHKTW_002626"
     CDS             10415..10597
                     /gene="ycaR"
                     /locus_tag="ACHKTW_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HFO6463626.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            10594..11340
                     /gene="kdsB"
                     /locus_tag="ACHKTW_002627"
     CDS             10594..11340
                     /gene="kdsB"
                     /locus_tag="ACHKTW_002627"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HFO6463627.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHEDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAEGLETVGDNFLRHLGIYGYRAGFIRRYVNWQASPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            11494..12387
                     /gene="ycbJ"
                     /locus_tag="ACHKTW_002628"
     CDS             11494..12387
                     /gene="ycbJ"
                     /locus_tag="ACHKTW_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HFO6463628.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(12364..13143)
                     /gene="elyC"
                     /locus_tag="ACHKTW_002629"
     CDS             complement(12364..13143)
                     /gene="elyC"
                     /locus_tag="ACHKTW_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HFO6463629.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTGKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGPSGEPRQE"
     gene            13279..14064
                     /gene="cmoM"
                     /locus_tag="ACHKTW_002630"
     CDS             13279..14064
                     /gene="cmoM"
                     /locus_tag="ACHKTW_002630"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HFO6463630.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDHVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     HQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            14061..15383
                     /gene="mukF"
                     /locus_tag="ACHKTW_002631"
     CDS             14061..15383
                     /gene="mukF"
                     /locus_tag="ACHKTW_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HFO6463631.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            15364..16068
                     /gene="mukE"
                     /locus_tag="ACHKTW_002632"
     CDS             15364..16068
                     /gene="mukE"
                     /locus_tag="ACHKTW_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HFO6463632.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEENQPDSGEEE"
     gene            16068..20528
                     /gene="mukB"
                     /locus_tag="ACHKTW_002633"
     CDS             16068..20528
                     /gene="mukB"
                     /locus_tag="ACHKTW_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HFO6463633.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVSKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEADLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADCAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFTS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLPGSDEAPSQAS"
     gene            20789..22636
                     /gene="ldtD"
                     /locus_tag="ACHKTW_002634"
     CDS             20789..22636
                     /gene="ldtD"
                     /locus_tag="ACHKTW_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFO6463634.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPVKGTRWLYSSKPYALATPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLALLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLVYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWQVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADGRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            22817..23365
                     /gene="mepK"
                     /locus_tag="ACHKTW_002635"
     CDS             22817..23365
                     /gene="mepK"
                     /locus_tag="ACHKTW_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HFO6463635.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            23392..24039
                     /gene="gloC"
                     /locus_tag="ACHKTW_002636"
     CDS             23392..24039
                     /gene="gloC"
                     /locus_tag="ACHKTW_002636"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HFO6463636.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDD
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(24261..25451)
                     /gene="aspC"
                     /locus_tag="ACHKTW_002637"
     CDS             complement(24261..25451)
                     /gene="aspC"
                     /locus_tag="ACHKTW_002637"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HFO6463637.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGIPEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSETVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCEAI
                     VAVL"
     gene            complement(25636..26724)
                     /gene="ompF"
                     /locus_tag="ACHKTW_002638"
     CDS             complement(25636..26724)
                     /gene="ompF"
                     /locus_tag="ACHKTW_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HFO6463638.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
BASE COUNT         6564 a         6606 c         7314 g         6543 t
ORIGIN      
        1 gattcacatg aagaatacta aattactgct ggcgattgcg acctctgcag cattactgac
       61 agggtgtcaa aatacccacg gtattgatac caatatggct atcagctccg gtttaaatgc
      121 ctataaagca gcaacattaa gcgatgccga tgcaaaagcg attgccaatc agggctgtgc
      181 cgaaatggac agcggcaatc aagtcgcaag taaatccagc aagtacggta aacgtctggc
      241 aaaaatcgcc aaagcattgg gtaacaatat taatggcacg ccggtcaact ataaggttta
      301 tatgaccagc gacgtcaacg catgggcgat ggcgaacggc tgtgtccgtg tctacagtgg
      361 cctgatggac atgatgaatg acaacgaaat tgaaggtgtt ctgggccatg aactgggcca
      421 cgtcgcgttg ggtcactcgc tggctgaaat gaaagcttct tatgcgatcg ttgccgcacg
      481 cgatgccatt tcagctacca gcggagtggc ttcccagctt tcccgctcac aattgggtga
      541 tatcgcagaa ggcgctatta atgctaaata ctcccgtgat aaagagtccg aagcagatga
      601 tttctccttt gatctgttga agaaacgtgg catcagcacc caggggctgg ttggcagctt
      661 tgaaaaactg gctagtctgg atggcggtcg cacccagtcc atgtttgact ctcacccacc
      721 atcaacagag cgtgcgcaac acatccgtga tcgtatcgcc tctggtaagt aaatcattgt
      781 catctttcgg gctggtcttc tgccagcccg ctataattgc gcaataaatc cccatctgaa
      841 tacagacaaa actggttttt gcacacaacg ttaacgattt gtggcgtcgg cgcgtataat
      901 gcgcgcggtt atgttaacgg tacgcctgtt ttaaggagat aaagatgacg gcaattgccc
      961 cggttattac cattgatggc ccaagcggtg cagggaaagg caccttgtgt aaggctatgg
     1021 cggaagcatt gcaatggcat ctgctggatt cgggtgcaat ttatcgcgta ctggcattgg
     1081 cggcattaca tcaccatgtt gatgttgcgt cagaagatgc gctggtaccg ctggcatccc
     1141 atctggatgt acgttttgtg tcaaccaatg gcaatctgga agtgatcctc gaaggggaag
     1201 atgtcagcgg cgaaattcgt actcaggaag tggcgaatgc agcttcacaa gtcgcggcat
     1261 tcccacgcgt tcgtgaagca ttattgcgtc gccaacgcgc gtttcgcgaa ttaccaggcc
     1321 tgattgccga tggccgcgac atgggaacgg tggtattccc tgatgcgcca gtgaaaattt
     1381 tccttgacgc ctcctcggaa gaacgtgcgc atcgccgcat gctacagttg caggagaagg
     1441 gctttagtgt taactttgag cgccttttgg ccgagatcaa agaacgcgac gaccgcgatc
     1501 gtaaccgagc ggtagcgcca ctggttccgg cagccgatgc tttagtgttg gattccacca
     1561 ccttaagcat tgagcaagtg attgaaaaag cgctacaata cgcgcgccag aaattggctc
     1621 tcgcataagc gaccgaattt gcagtacccc cgttgcaatg gaatgacagc gggtatgtta
     1681 aacaacccca tccggcatgg agccaggtgg acgttaaata taaacctgaa gattaaacat
     1741 gactgaatct tttgctcaac tctttgaaga gtccttaaaa gaaatcgaaa cccgcccggg
     1801 ttctatcgtt cgtggcgttg ttgttgctat cgacaaagac gtagtactgg ttgacgctgg
     1861 tctgaaatct gagtccgcca tcccggctga gcagttcaaa aacgcccagg gcgagctgga
     1921 aatccaggta ggtgacgaag ttgacgttgc tctggacgca gtagaagacg gcttcggtga
     1981 aactctgctg tcccgtgaga aagctaaacg tcacgaagcc tggatcacgc tggaaaaagc
     2041 ttacgaagat gctgaaactg ttaccggtgt tatcaacggc aaagttaagg gcggcttcac
     2101 tgttgagctg aacggtattc gtgcgttcct gccaggttct ctggtagacg ttcgtccggt
     2161 gcgtgacact ctgcacctgg aaggcaaaga gcttgaattt aaagtaatca agctggatca
     2221 gaagcgcaac aacgttgttg tttctcgtcg tgccgttatc gaatccgaaa acagcgcaga
     2281 gcgcgatcag ctgctggaaa acctgcagga aggcatggaa gttaaaggta tcgttaagaa
     2341 cctcactgac tacggtgcat tcgttgatct gggcggcgtt gacggcctgc tgcacatcac
     2401 tgacatggcc tggaaacgcg ttaagcatcc gagcgaaatc gtcaacgtgg gcgacgaaat
     2461 cactgttaaa gtgctgaagt tcgaccgcga acgtacccgt gtatccctgg gcctgaaaca
     2521 gttgggcgaa gatccgtggg tagctatcgc taaacgttat ccggaaggta ccaaactgac
     2581 tggtcgcgtg accaacttga ccgactacgg ctgcttcgtt gaaatcgaag aaggcgttga
     2641 aggcctggta cacgtttccg aaatggattg gaccaacaaa aacatccacc cgtccaaagt
     2701 tgttaacgtt ggcgatgtag tggaagttat ggttctggat atcgacgaag aacgtcgtcg
     2761 tatctccctg ggtctgaaac agtgcaaagc taacccgtgg cagcagttcg cggaaaccca
     2821 caacaagggc gaccgtgttg aaggtaaaat caagtctatc actgacttcg gtatcttcat
     2881 cggcctggac ggcggcatcg acggcctggt tcacctgtct gacatctcct ggaacgttgc
     2941 aggcgaagaa gcagttcgtg aatacaaaaa aggcgacgaa atcgctgcag ttgttctgca
     3001 ggttgacgca gaacgtgaac gtatctccct gggcgttaaa cagctcgcag aagatccgtt
     3061 caacaactgg gttgctctga acaagaaagg cgctatcgta accggtaaag taactgcagt
     3121 tgacgctaaa ggcgcaaccg tagaactggc tgacggcgtt gaaggttacc tgcgtgcttc
     3181 tgaagcatcc cgtgaccgcg ttgaagacgc taccctggtt ctgagcgttg gcgacgaagt
     3241 tgaagctaaa ttcaccggcg ttgatcgtaa aaaccgcgca atcagcctgt ctgttcgtgc
     3301 gaaagacgaa gctgacgaga aagatgcaat cgcaactgtt aacaaacagg aagatgcaaa
     3361 cttctccaac aacgcaatgg ctgaagcttt caaagcagct aaaggcgagt aattctctga
     3421 ctcttcggga tttttattcc gaagtttgtt gagtttactt gacagattgc aggtttcgtc
     3481 ctgtaatcaa gcactaaggg cggctacggc cgcccttaat caatgcagca acagcagccg
     3541 cttaatttgc ctttaaggaa ccggaggaat catgaccaag tcagaattga tagaaagact
     3601 tgccacccag caatcgcaca ttcccgccaa gacggttgaa gatgcagtaa aagagatgct
     3661 ggagcatatg gcctcgactc ttgcgcaggg cgagcgtatt gaaatccgcg gtttcggcag
     3721 tttctctttg cactaccgcg caccacgtac cggacgtaat ccgaagactg gcgataaagt
     3781 agaactggaa ggaaaatacg ttcctcactt taaacctggt aaagaactgc gcgatcgcgc
     3841 caatatttac ggttaagttt ttactcaaac ttgaacgcga gaaaagcacc tgtcgggtgc
     3901 ttttttcatt tctctaatca ggaactggaa gctgcctcgc agagttttga gcagttttca
     3961 ccctttcgtt aaattcttct gaatgtgcct cggggaacgc aaaattccca ccagacaacc
     4021 gctcaacaaa gttgcacact ttccataaac agggaggggt gcatgaaaat aacgacagtc
     4081 ggcgtatgca taatttgcgg aatttttccg ttgctgattt tgccccaatt gcctgggaca
     4141 ttaacccttg cgtttctgac tctcttcgcc tgcgtactgg ctttatccct gttaaaaccg
     4201 tccgttatat cgcgctgacg ttgctgtttt tcgtttgggg catattatca gcaaagcaaa
     4261 ttttgtgggc aggagaaacc ttaactggcg cgacgcagga tgcaattgtt gagatcactg
     4321 caactgacgg catgaccact cattacggtc aaattactca tctacaaggt cgacgtatat
     4381 tccctgcgcc aggccttgtg ctgtatggcg aatatcttcc gcaagcggtt tgtgctggac
     4441 aacaatggtc aatgaaactc aaagttcgtg cggttcatgg ccaacttaat gatggcggct
     4501 ttgatagcca gcgttatgcc attgcccagc atcaaccgct caccggccgc tttctgcagg
     4561 caagtgttat tgaaccgaat tgtagcctgc gtgcacagta tctggcgtca ctacaaacaa
     4621 cgctgcaacc ctatccgtgg aatgcggtta ttcttggttt aggtatgggg gaacggttat
     4681 ccgtccccaa agaaatcaaa aatatcatgc gcgatactgg aacggcgcat ttaatggcga
     4741 tatcgggatt gcacatcgct tttgcggcgt tgctggctgc cggactcatt cgcagtggac
     4801 aaatttttct gcctgggcgc tggatccact ggcaaatacc attaattggt gggatctgct
     4861 gtgctgcttt ttatgcctgg ttgacgggaa tgcaacctcc tgcattgcgt accgtggtgg
     4921 cgcttgctac gtggggaatg cttaagttaa gtgggcgaca gtggagtggc tgggatgtat
     4981 ggatatgttg tctggcggca attttgctga tggatcctgt tgccattctc tcgcaaagtt
     5041 tatggctctc tgccgctgcg gtcgcggcac tgattttttg gtatcagtgg tttccctgtc
     5101 ctgagtggca actgccgccg gtattgcgtg cagttgtttc cctcatccat ctgcaactgg
     5161 gtatcacact tctgcttatg cccgtgcaaa tcgtcatatt tcatggcatt agtctgacct
     5221 cgtttattgc aaatctatta gcaattccct tggtgacatt tatcacggtt ccgttgatcc
     5281 tcgccgcgat ggttgtgcat ttaagcgggc cgttaatcct ggagcaaggg ttatggtttc
     5341 ttgccgaccg gtctttggct ttacttttct gggggttaaa gagtttgcca gaagggtgga
     5401 tcaacattgc tgaacgttgg caatggctat cattttcccc atggttctta ctggtggtat
     5461 ggcgattaaa cgcctggcga acgttgccag caatgtgtgt ggctgtaggc ttgctgatgt
     5521 gctggccgct atggcaaaaa cctcgacctg acgagtggca ggtgtacatg cttgatgtcg
     5581 ggcaagggct ggcaatggtg atagccagaa acggcaaagc gattctctat gacacaggac
     5641 tggcctggcc cgaaggggat agtgggcaac aactgattat cccctggctc cactggcata
     5701 atcttgaacc ggaaggcgtt attctgagtc atgaacatct ggatcaccgg ggagggctgg
     5761 actcaatatt gcacacatgg ccgatcttat ggatcagaag tccgttagac tgggaacatc
     5821 atcagccctg tgtgcgtggc gaagcgtagc aatggcaagg attgcgtttc agcgtgcact
     5881 ggcctttaca agctagcaac gataaaggaa ataaccattc ctgtgtggtt aaggttgatg
     5941 acgggacgaa tagcattctt ctaaccggtg atattgaagt ccccgctgaa caaaagatgc
     6001 taagccgtta ctggcagcaa gtgcagacaa cattgcttca ggtacctcac catggcagta
     6061 atacctcatc atcgttgcca ttaattcagc gagtgaatga aaaagtggca ctcgcatcgg
     6121 catcgcgcta taacgcatgg cgattgccct ctaataaagt taagcatcgc tatcaacagc
     6181 aaggatatca atggcttgat actccacatc agggtcaagt gacggtcaat ttttcagcgc
     6241 aaggctggcg gattagcagc ctcagagagc aaattttacc tcgttggtat catcagtggt
     6301 ttggcgtgcc agtggataac gggtagaata tgcggctatt tcaacaaatg ctggtttttt
     6361 gaatgcataa cgacaaagat ctctctacgt ggcagacgtt ccgccgactg tggccaacca
     6421 ttgcgccttt taaagcgggt ctgatcgtgg cgggcgtagc gttaatcctc aacgcagcca
     6481 gcgatacctt catgttatcg ctccttaagc cacttcttga tgatggcttt ggtaaaacag
     6541 atcgctccgt gctggtgtgg atgccgctgg tggtgatcgg gctgatgatt ttacgtggta
     6601 tcaccagcta tgtctccagc tactgtatct cctgggtatc aggaaaggtg gtaatgacca
     6661 tgcgtcgccg cctgtttggt cacatgatgg gaatgccggt ttcatttttt gacaaacagt
     6721 caacggggac gctgttgtca cgtatcacct acgattccga acaggttgct tcttcttctt
     6781 ccggcgcact gataacggtt gtgcgtgaag gtgcgtcaat catcggcctg ttcatcatga
     6841 tgttctatta cagttggcaa ctgtcgatca ttttgattgt gctggcaccg attgtttcga
     6901 ttgcgattcg cgtagtatcg aagcgttttc gcaacatcag taaaaacatg cagaacacca
     6961 tggggcaggt gaccaccagc gccgaacaaa tgctgaaggg ccacaaagaa gtattgattt
     7021 tcggtggtca ggaagtggaa acgaaacgct ttgataaagt cagcaaccga atgcgtcttc
     7081 aggggatgaa aatggtttca gcctcttcca tctctgatcc gatcattcag ctgatcgcct
     7141 ctttggcgct ggcgtttgtt ctgtatgcgg cgagcttccc aagtgtcatg gatagcctga
     7201 ctgccggtac gattaccgtt gttttctctt caatgattgc actgatgcgt ccgctgaaat
     7261 cactgaccaa cgttaacgcc cagttccagc gcggtatggc ggcttgtcag acgctgttta
     7321 ccattctgga cagtgagcag gagaaagacg aaggtaagcg cgtgatcgag cgtgcgactg
     7381 gcgacgtgga attccgcaat gtcaccttta cttatccggg acgtgacgta cctgcattgc
     7441 gtaacatcaa cctgaaaatt ccggcaggga agacggttgc tctggttgga cgctctggtt
     7501 caggtaaatc aaccatcgcc agcctgatca ctcgttttta cgatattgat gaaggcgaaa
     7561 tcctgatgga tggtcacgat ctgcgcgagt ataccctggc gtcgttacgt aaccaggttg
     7621 ctctggtgtc gcagaatgtc catctgttta acgatacggt tgctaacaac attgcttacg
     7681 cacggactga acagtacagc cgtgagcaaa ttgaagaagc ggcgcgtatg gcctacgcca
     7741 tggacttcat caataagatg gataacggtc tcgatacagt gattggtgaa aacggcgtgc
     7801 tgctctctgg cggtcagcgt cagcgtattg ctatcgctcg agccttgctg cgtgatagcc
     7861 cgattctgat tctggatgaa gctacctcgg ctctggatac cgaatccgaa cgtgcgattc
     7921 aggcggcact ggatgagttg cagaaaaacc gtacctctct ggtgattgcc caccgcttgt
     7981 ctaccattga aaaggcagac gaaatcgtgg tcgtcgagga tggtgtcatt gtggaacgcg
     8041 gtacgcataa cgatttgctt gagcaccgcg gcgtttacgc gcaacttcac aaaatgcagt
     8101 ttggccaatg atcgaaaaaa tctggtctgg tgaatcccct ttgtggcggc tattgctgcc
     8161 actctcctgg ttgtatggcc tggtaagtgg cgcgatccgt ctttgctata aactaaaact
     8221 gaagcgcgcc tggcgtgccc ccgtaccggt tgtcgtggtt ggtaatctca ccgcaggcgg
     8281 caacggaaaa accccggtcg ttgtctggct ggtggaacag ttgcaacagc gcggtattcg
     8341 cgtgggggtc gtatcgcggg gatatggtgg taaggctgaa tcttatccgc tgttattgtc
     8401 ggcagatacc acaacagcac aggcgggtga tgaacctgtg ttgatttatc aacgcactga
     8461 tgcgcctgtt gcggtttctc ccgttcgttc tgatgcggta aaagccattc tggcgcaaca
     8521 ccctgatgtg cagatcatcg taaccgacga cggtttacag cattaccgtc tggcgcgtga
     8581 tgtggaaatt gtcgttattg atggtgtgcg tcgctttggc aatggctggt ggttgccggc
     8641 ggggccaatg cgtgagcgag cggggcgctt aaagtcggtt gatgcggtaa tcgtcaacgg
     8701 cggtgtccct cgcagcggtg aaatccccat gcatctgctg ccgggtcagg cggtgaattt
     8761 acgtaccggt acgcgttgtg acgttgctca gcttgaacat gtggtggcga tggcagggat
     8821 tgggcatccg ccgcgctttt ttgccacgct gaagatgtgc ggcgtacaac cggaaaaatg
     8881 tgtaccgctg gccgatcatc agtctttgaa ccatgcggat gtcagcgcgt tggtaagcac
     8941 cgggcaaacg ctggtaatga ctgaaaaaga tgcggtgaaa tgccgggcct ttgcagaaga
     9001 aaattggtgg tatttgcctg tagacgcaca gctttcaggt gatgaaccag cgaaactgct
     9061 tacgcaacta acctcgctgg cttctggcaa ctagttacgc cgcggcagcg ttcgattgat
     9121 ggagtcatga atgtcgctgc cgcacctctc ccttgctgat gcgcgtaatc ttcaccttgc
     9181 cgcacaaggc ctgttaaaca aaccccgccg tcaagcgtcg ttggaggata ttccggcaac
     9241 gatctcccgc atgtccttgc tgcaaatcga taccatcaat attgttgccc gtagtccata
     9301 tctggtgctt ttcagtcgtc tgggaaatta tcctgcccag tggctggatg agtctctggc
     9361 gcgtggcgaa ttaatggaat actgggcgca tgaagcctgc tttatgccgc gtagcgactt
     9421 tcgtcttatt cgccaccgca tgctggcacc tgaaaaaatg ggctggaaat acaaagacgc
     9481 ctggatgcag gaacatgagg cggaaattgc acagttaatt cagcatattc atgataaggg
     9541 gccggtacgt tcagccgatt ttgagcatcc tcgtaaaggt gcaagcggct ggtgggaatg
     9601 gaagccgcat aaacggcatc tggaaggttt atttactgcc ggaaaggtga tggtgattga
     9661 acggcgcaac ttccagcgcg tttatgattt aacccaccgt gtcatgcctg actgggatga
     9721 tgagcgcgat ctcgtttcgc aaacagaagc agaaatcatc atgctggata acagtgcgcg
     9781 tagcctggga atattccgcg aacagtggct ggcagattac tatcggctga aacgtccggc
     9841 actggcggcg tggcgcgaag cgagggctga acagcagcaa atcattgctg tgcatgttga
     9901 aaaattgggc aatctttggc tgcatgctga tttgctgccg ctactcgagc gtgcgctggc
     9961 cggaaagctc actgcaacgc acagcgcggt actttcgcct tttgatcctg ttgtctggga
    10021 tcgcaaacgc gcagagcagc tttttgattt tagctaccgg ctggagtgct ataccccagc
    10081 gccgaaacgc cagtatggct attttgttct gccattatta catcgtgggc aattagttgg
    10141 gcgaatggat gccaaaatgc atcgccagac aggcatcctt gaagttatct ctctgtggtt
    10201 acaggaaggc attaaaccaa cgacaatgct gcaaaaaggg ttacgtcagg cgattactga
    10261 tttcgcgagc tggcagcagg caacgcgggt gacattagga cgctgcccgc aaggtctctt
    10321 tactgattgc cgcactggct gggaaataga ccccgtcgca taaatgatta tgataagatt
    10381 tatgcattca taacccgatc catctggagg aactatggat catcgtttgc ttgaaatcat
    10441 tgcctgcccg gtttgcaacg gaaaactttg gtataaccag gaaaaacaag agcttatttg
    10501 caaactggat aacctggctt tcccgctccg cgatggcatt ccagtgttgc tggaaacgga
    10561 agcccgcgtg ctgactgctg atgagagtaa atcatgagtt ttgtggtcat tattcccgcg
    10621 cgctacgcgt ccacgcgtct gcccggtaaa ccattggttg atattaacgg caaacccatg
    10681 attgttcatg ttcttgaacg cgcgcgtgaa tcaggtgccg agcgcatcat cgtggcaacc
    10741 gatcatgagg atgttgcccg cgccgttgaa gccgctggcg gtgaagtatg tatgacgcgc
    10801 gccgatcatc agtcaggaac agaacgtctg gcggaagttg tcgaaaaatg cgcattcagc
    10861 gacgacacgg tgatcgttaa tgtgcagggt gatgaaccga tgatccctgc gacaatcatt
    10921 cgtcaggttg ctgataacct cgctcagcgt caggtgggta tggcgactct ggcggtgcca
    10981 atccacaatg cggaagaagc gtttaacccg aatgcggtga aagtggttct cgacgctgaa
    11041 gggtatgcac tgtacttctc acgcgccacc attccttggg atcgtgatcg ttttgcagaa
    11101 ggccttgaaa ccgttggcga taacttcctg cgtcatcttg gtatttatgg ctaccgtgca
    11161 ggctttatcc gtcgttacgt caactggcag gcaagtccgt tagaacacat cgaaatgtta
    11221 gagcagcttc gtgttctgtg gtacggcgaa aaaatccatg ttgctgttgc tcaggaagtt
    11281 cctggcacag gtgtggatac ccctgaagat cttgagcgcg ttcgcgctga aatgcgctaa
    11341 tttcacttca cgacacttca gccaattttg ggaggagtgt cgtaccgtta cgattttcct
    11401 caatttttct tttcaacaat tgatctcatt caggtgacat cttttatatt ggcgctcatt
    11461 atgaaagcag tagcttttat gagggtaatc tgaatggaac agctgcgtgc cgaattaagc
    11521 catttactgg gcgaaaaact cagtcgtatt gagtgcgtca atgaaaaagc ggatacggcg
    11581 ttgtgggctt tgtatgacag ccagggaaac ccaatgccgt taatggcaag aagctttagt
    11641 acgcccggaa aagcccgaca actggcatgg aaaaccacca tgctggcaag aagtgggact
    11701 gtccgtatgc cgactattta tggtgtgatg acgcatgaag aacaccccgg ccccgatgtc
    11761 ctgctactgg agcggatgcg tggtgtttcg gtggaggcac cagcccgaac accagaacgc
    11821 tgggaacaac tcaaagacca aatcgttgaa gccttactgg cctggcaccg tcaggacagt
    11881 cgcggttgcg tcggcgcggt cgacaatact caggaaaatt tctggccctc atggtaccgg
    11941 caacatgttg aagtgctatg gaccacgctc aatcagttca ataacaccgg tctgacgatg
    12001 caggataagc ggatcctgtt tcgcactcgc gaatgtctcc cggcattatt tgaaggcttt
    12061 aacgacaatt gtgtgttgat tcacggtaac ttctgtttac gcagcatgct gaaagattcg
    12121 cgcagcgatc agttactggc gatggtcggg ccgggactaa tgctttgggc accgcgagaa
    12181 tacgaactgt tccgactaat ggataattct ctggcggaag atttgctctg gagttacctg
    12241 caacgcgcgc cagtggcgga gtcgttcatc tggcggcgtt ggttgtatgt gttatgggat
    12301 gaagttgcgc aactggttaa taccggacga tttagtcggc gcaacttcga tctggcatca
    12361 aaatcactct tgccgtggct cgcctgacgg acctttcagc cactgccaga tgcggccaag
    12421 cgtttcatag ccaacacgat cgctatgcat cagccagacc ggagaaggga ttgcccgttc
    12481 ccacggatta agcggcgagt cgatggccaa ctgattcgcc ggggctggta gcggatttaa
    12541 cccttcctgc tgaaaaaaga tcatcgcccg cggcagatgt gatgcggagg tcaccagcag
    12601 gaaaggggcg tcaccaatcg cctgtttcac tgccgcagct tcttcttcgg tatcttttgg
    12661 caaatccagg gtgataattt gctcgcgcgg cacgcccagc gattgcgcaa ctctggcacc
    12721 tacttccgct gtactcaccg tattggtttt tgctacgcct cccgtgaaga tcagttttga
    12781 tcccggattt tcgcgccata agcgaatacc ttcattcagg cgtggcagac tgttattgat
    12841 taaattagag ctcggtgccc actgcgggtt ccaggtataa ccacctccga gcaccacgat
    12901 atagtccact ttttgcgaat tattccatgt cggataggtg ctttcgatgg gacgcagcag
    12961 gcggtcggct accggttgca aactcaataa caagagtgcc agccacccaa tgctaataaa
    13021 aatcttcccg gttttttgaa aacggctaaa ccacagcagc gccaggccag cgccaataat
    13081 taacaacatc agcggaagtg gcagcaacat attgccaatc acttttttca gtgtaaaaag
    13141 catcctttct ggttcctttt ttaaccatat agcaagggat cgccagcgat attacaccag
    13201 acgggttcat tctcgccgtg gctgtgacaa aatagcgtct ttatcagaaa acgtcttagc
    13261 caaccggtgg agaaagcaat gcaggatcgc aattttgatg atattgcgga aaagttttcc
    13321 cgtaacattt acggcaccac caaagggcag cttcgacagg ctattctgtg gcaggatctc
    13381 gatcacgtgc tggcggaaat gggcccgcaa aaactgcgtg tgctggatgc tggcggtgga
    13441 gaagggcaga ccgcaatcaa aatggccgag cgtgggcatc aggtcatttt atgcgatctt
    13501 tctgcgcaga tgatcgaccg cgcaaaacag gcggcagaag caaaaggtgt gagcgacaac
    13561 atgcaattta tacattgcgc cgctcaggat gttgcttcgc atttggaaac gcccgttgat
    13621 ctgatattgt tccatgcggt gctcgagtgg gtggccgatc cccgcagtgt attgcagacc
    13681 ctgtggtcag tgttgcgtcc aggcggcgtg ttgtcgttaa tgttctacaa tgcgcatggt
    13741 ttgttgatgc ataacatggt cgccgggaat tttgattacg tgcaggcggg aatgccgaaa
    13801 aagaaaaaac ggacgctttc gccagattat ccacgcgacc cggcgcaggt ttatctgtgg
    13861 ctggaagaag ctggttggca aattatgggt aagacaggcg ttcgcgtgtt tcatgattat
    13921 ctgcgcgaga agcaccagca gcgcgactgc tatgaagcat tacttgaatt agaaacgcgt
    13981 tattgccgtc aggaaccgta tattaccctg gggcgttata ttcatgtcac cgcgcgcaaa
    14041 ccgcagagca aggataaagt atgagtgaat tttcccagac agtccccgaa ctggttgcct
    14101 gggccagaaa aaatgacttc tccatctcgc tgccggtaga ccgactctct tttctgctgg
    14161 cggttgccac gctgaacggc gagcgtctgg atggtgagat gagtgaaggc gagctggtgg
    14221 atgcattccg ccatgtgagt gatgcgtttg agcaaaccag cgaaaccatc ggcgtgcgcg
    14281 ccaataacgc gatcaacgac atggtgcgtc aacgtctgct gaaccgcttt accagcgagc
    14341 aggcggaagg gaacgcaatt taccgtctga cgccgctcgg catcggcatt actgactact
    14401 acatccgtca gcgcgagttt tctacgctgc gtctttctat gcagttgtcg attgtggcgg
    14461 gtgagctcaa acgcgcagca gatgccgccg aagagggcgg tgatgaattt cactggcacc
    14521 gtaatgtcta tgcgccactg aaatattcgg tagcagaaat tttcgacagt atcgacctga
    14581 cgcaacgtct gatggacgaa cagcagcagc aggtgaagga cgatatcgcc cagttgctga
    14641 acaaagactg gcgggcggcg atttccagct gtgaattgtt gctttcggaa acttccggaa
    14701 cgctgcgtga attgcaggat acgctggaag cggcaggcga caaattgcag gctaatctgt
    14761 tgcgcattca ggatgcgacg atgacccatg acgatctgca tttcgtcgat cgtctggtgt
    14821 tcgatctgca gagcaaactc gatcgtatta tcagttgggg ccagcaatcc atcgacttgt
    14881 ggattggcta cgaccgccac gtacacaaat ttattcgtac cgcgatcgat atggataaaa
    14941 accgcgtctt tgctcagcgg ttacgtcagt cggtacaaac ctattttgat gagccgtggg
    15001 cgctaactta tgccaatgcc gatcgtctgc tggatatgcg tgacgaagag atggcactgc
    15061 gcgatgaaga agtgactggg gaacttcctg aggatctgga atacgaagag tttaacgaga
    15121 tccgcgaaca gctggcggcg atcatcgaag aacaacttgc cgtgtacaaa accagacaag
    15181 tgccgctgga tcttggtctg gtggtacgcg aatatctgtc acagtatccg cgtgcacgtc
    15241 actttgacgt tgcgcgcatt gttattgatc aggcggtacg tcttggcgta gcgcaagcag
    15301 atttcaccgg actgccagcg aaatggcagc cgattaatga ttacggagcc aaggtacagg
    15361 cgcatgtcat cgacaaatat tgaacaagtg atgccggtta agctggcgca ggcgctggcg
    15421 aatccgttat ttccggcgct ggacagcgcc ttacgttcag gccgccatat tggcctcgac
    15481 gaactggata atcatgcatt cctgatggat tttcaggaat atctggaaga gttttacgcg
    15541 cgttataacg ttgagcttat tcgcgcacca gaagggttct tctatttacg cccacgttcc
    15601 accacgctga tccctcgttc cgtcttgtcg gaactggata tgatggtcgg gaaaatcctc
    15661 tgttatctct atctcagccc ggaacggctg gcgaatgagg ggattttcac ccagcaggaa
    15721 ctgtacgacg aactgctcac cctggccgat gaagcaaaac tgctgaaact ggtgaacaac
    15781 cgttcaaccg gttcagacgt tgaccgtcag aagttgcagg agaaagtacg ttcttcgctc
    15841 aaccgtctgc gtcgtttagg catggtgtgg tttatgggcc acgacagcag caagtttcgc
    15901 attaccgaat cggtgttccg cttcggagcc gatgtgcgtg ctggcgacga tccccgtgaa
    15961 gcacagcgtc gcctgattcg tgatggcgaa gcaatgccga ttgaaaatca tctgcaactc
    16021 aacgatgaaa ccgaagagaa tcagccagat agcggagagg aagaataatg attgaacgcg
    16081 gtaaatttcg ctcactgacg ctgattaact ggaacggctt ttttgcccga acttttgacc
    16141 ttgacgagct ggtcacgacg ctttctggcg gtaacggggc gggtaaatcc accaccatgg
    16201 cggcgttcgt tacggcgctg atccccgacc tgaccctgct gcatttccgt aacactacgg
    16261 aagccggggc caccagcggt tcgcgcgata aaggtctgca cggtaagctg aaagcgggtg
    16321 tctgttattc gatgctcgac accattaact cgcgccacca gcgcgtggtg gtcggtgtgc
    16381 gtctgcaaca ggttgccgga cgcgatcgta aagtggatat caagccgttt gccattcagg
    16441 gactgccgat gtcggtgcag ccgacacagc tggtgaccga aaccctgaac gaacgccagg
    16501 cgcgcgtgct gccgcttaac gagctgaaag acaagctcga ggcgatggaa ggcgtgcagt
    16561 ttaaacagtt caactccatt actgattacc actcgctgat gttcgatctg ggcatcatcg
    16621 cgcgtcgtct gcgctccgca tctgaccgta gcaaattcta tcgtctgatc gaagcttcgc
    16681 tgtatggcgg gatctccagt gccattaccc gttctctgcg cgactacctg ttgccggaaa
    16741 acagcggcgt gagtaaagcg ttccaggaca tggaagcggc gctgcgtgaa aaccgtatga
    16801 cgctggaagc gattcgtgtc acccagtcgg accgcgacct gtttaagcat ctgatcagcg
    16861 aagccaccaa ctacgtggcg gcggactaca tgcgtcacgc caacgagcgc cgtgtccatc
    16921 tcgacaaagc cctggagttt cgtcgcgagc tacatacttc gcgtcagcaa ctggcggctg
    16981 agcagtacaa acacgtcgat atggcgcgtg agctggcaga gcacaacggt gccgaaggcg
    17041 atctggaagc ggattatcag gcggccagcg atcacctgaa cctggtgcaa accgcactgc
    17101 gtcagcagga gaagatcgaa cgctacgaag cggatctcga tgagctgcag atccgtctgg
    17161 aagagcaaaa tgaagtggtg gcagaagcca tcgaacgcca ggaagagaat gaggctcgtg
    17221 cggaagctgc cgaactggaa gtggacgagc tgaaaagcca gcttgctgac taccagcagg
    17281 cgctggacgt ccagcaaacg cgcgcgatcc agtataacca ggcgattgct gcgcttaatc
    17341 gtgccaaaga actgtgccat ctgccggact taaccgccga ctgcgccgcc gaatggctgg
    17401 aaaccttcca ggcgaaagag ctggaagcga ctgaaaaaat gctctctctt gagcagaaaa
    17461 tgagcatggc gcaaaccgcg cacagccagt ttgagcaggc ttatcagctg gtggtggcaa
    17521 tcaacggccc gctggcgcgt aacgaggcgt gggatgtcgc tcgcgaacta ttgcgcgaag
    17581 gggtcgatca gcgtcacctg gcagagcagg ttcaaccgtt gcggatgcgc ttaagtgaac
    17641 tggaacagcg tctgcgcgag cagcaagaag ccgaacgcct gctggcagac ttctgcaaac
    17701 gtcagggcaa gaattttgat attgatgaac tggaagctct gcatcaggag ctggaagcgc
    17761 gcattgcctc tctttccgat agcgtgtcta acgcccgtga agagcgcatg gcactgcgcc
    17821 aggaacagga acagctgcag tctcgcattc agagtttgat gcagcgtgcg ccggtttggc
    17881 tggcagcgca aaacagtctc aaccagttga gcgagcagtg cggtgaagag tttacctcca
    17941 gccaggacgt caccgaatat ctgcaacagt tgctggagcg tgagcgagag gcgattgttg
    18001 aacgcgatga agtgggcgcg cgcaaaaacg ctgtcgatga agagatcgaa cgtttaagcc
    18061 agcctggcgg ctctgaagat cagcgtctga acgcgctggc ggagcgtttt ggtggtgtgc
    18121 tgctgtcaga aatttatgac gacgttagcc tggaagatgc gccgtacttc tcagcgctgt
    18181 atggcccgtc acgccacgcc atcgtggtgc cagatctgtc acaggtaact gaacacctgg
    18241 aaggcttgac cgattgcccg gaagatctct atctgatcga aggagatccg cagtcattcg
    18301 atgacagcgt gttcagcgtt gatgagctgg aaaaagcggt agtggtgaaa atcgccgatc
    18361 gtcagtggcg ttattcacgt ttcccggaag tgccgctgtt tggtcgtgct gcgcgtgaaa
    18421 gccgtattga aagcctccat gccgagcgtg aagtgctttc cgaacgcttc gccacgctct
    18481 cctttgatgt acagaaaact cagcgtctgc atcaggcgtt cagccgcttt atcggcagtc
    18541 atctggcggt tgcgtttgag tctgacccgg aagcagaaat ccgtcaactg aacagccgtc
    18601 gcgtcgaact ggagcgggcg ttaagtaatc atgaaaatga taaccagcag cagcgtattc
    18661 agtttgagca ggcgaaagag ggcgttacgg cgctgaaccg cattctgccg cgtctcaacc
    18721 tgttggctga tgacagcctg gcggatcgcg tcgatgaaat ccgcgaacgt ctggatgaag
    18781 cccaggaagc cgcgcgtttt gttcagcagt ttggcaatca actggcgaaa ctggaaccga
    18841 ttgtttcggt attgcagagc gacccggaac agttcgaaca gttaaaagaa gattacgcgt
    18901 actctcagca gatgcagcgc gatgcccgtc agcaggcgtt tgccctgacg gaagtggtgc
    18961 agcgtcgtgc gcactttagc tattctgact cggcagaaat gcttagcggt aacagcgatc
    19021 tcaacgaaaa actgcgtgaa cgtctggaac aggcggaagc ggagcgtacc cgcgctcgcg
    19081 aagcgttgcg cggtcacgca gcgcagttga gtcagtacaa ccaggtgctg gcttcgctga
    19141 aaagttctta cgacaccaaa aaagagctac tcaacgatct gcaacgtgaa ttgcaggata
    19201 tcggcgtgcg tgctgatagc ggggcagaag agcgggcgcg tattcgccgt gacgagctgc
    19261 atgcgcaact gagcaataac cgttcacgcc gcaatcaact ggaaaaagcg cttaccttct
    19321 gcgaagcgga gatggacaac ctgacccgca aactgcgcaa gctggagcgg gattactttg
    19381 agatgcgcga gcaggtagtg accgccaaag cgggctggtg tgcggtgatg cgcatggtga
    19441 aagataacgg cgttgagcgc cgcttacacc gtcgtgagct ggcttatctc tccgctgatg
    19501 atttgcgttc catgtcggat aaggcgttag gtgcgctgcg tctggcggtg gcggataacg
    19561 aacatctgcg cgacgtgctg cgcatgtcgg aagatccgaa acgtccggag cgtaaaattc
    19621 agttcttcgt ggcggtttat cagcatctgc gtgaacgtat tcgtcaggat attattcgta
    19681 ccgatgatcc ggtggaagct atcgaacaga tggagattga acttagccgt ctgaccgaag
    19741 aattaacctc ccgtgaacag aaactggcga tcagttcccg cagcgtggcg aacatcattc
    19801 gcaaaaccat tcagcgcgag cagaaccgta tccgtatgct caaccagggg ttgcagaacg
    19861 tatcgtttgg tcaggtgaac agcgtgcgtc tcaacgtgaa cgtgcgtgaa acgcacgcca
    19921 tgctactgga tgtgctctct gaacagcacg agcagcatca ggatctgttt aacagcaacc
    19981 gtttgacctt ctcggaagcg ctggcgaaac tgtatcaacg tcttaacccg cagattgata
    20041 tggggcagcg cacgccgcag accatcggtg aagaactgct ggattaccgc aactatctgg
    20101 aaatggaagt tgaggttaac cgtggttccg atggctggct gcgcgcagag tctggtgcat
    20161 tgtcgaccgg tgaggcgatt ggtaccggta tgtcgattct ggtgatggtg gtacaaagct
    20221 gggaagatga atctcgccgc ctgcgcggta aagatatctc tccttgccgc ctgctgttcc
    20281 tcgatgaagc agcgcgactg gatgctcgtt ctatcgccac gctgtttgaa ttgtgtgaac
    20341 gtttgcaaat gcagctcatc atcgcagcgc cggaaaatat cagcccggag aaaggcacca
    20401 cctataaact ggtgcgtaaa gtcttccaga ataccgaaca cgttcacgtc gtcggcctgc
    20461 gaggatttgc gccgcaactc cctgaaacgc ttccaggaag tgacgaagcg ccttctcagg
    20521 cgagttaaaa ttaaggcggc agcaatgccg ccttttcttt ttccgaaaac tccgtttctg
    20581 cactaaaaaa gtggcacatt acggcgcgct tttctttaaa cttctttaca ttaggttatg
    20641 taaaaacgta tcggcgttta tatactgaag ataagcctga tgagtaacag gcttgctcgt
    20701 catactttcg tgagtattgg cgttgtacag gcaagtcgta aaataacagc ctggctattc
    20761 agagtatgat aaaaacaggg ggcaagggat gttgcttaat atgatgtgtg gtcgtcggct
    20821 gtcggcaatc agtttgtgct tggccgtaac attcgctcca ctgttcaatg cgcaggccga
    20881 tgagcctgaa gtaatccctg gcgacagccc ggtggctgtc agtgaacagg gcgaggcact
    20941 gccgcaggcg caagccacgg caataatggc ggggatccag ccattgcctg aaggtgcggc
    21001 agaaaaagcc cgcacgcaaa tcgaatctca attacccgca ggttacaagc cggtttatct
    21061 taaccagctt caactgttgt atgccgcacg cgatatgcaa cccatgtggg aaaaccgtga
    21121 tgctgttaaa gccttccagc aacagctggc agaggtggcg attgccggtt tccagccgca
    21181 gtttaataaa tgggtagagt tactgaccga tcctggtgtt aacgggatgg cacgcgacgt
    21241 agtgctctct gatgcgatga tgggctatct ccatttcatt gcaaatattc cggtcaaagg
    21301 cactcgctgg ctatatagca gtaaacctta tgcacttgca acgccgccgc tctcggtgat
    21361 taaccaatgg cagctggcgc tggataaagg tcaattgcct acgtttgttg caggactggc
    21421 accgcagcat ccgcaatatg cggcgatgca tgaatcgtta ctggccttac tcagtgacac
    21481 caaaccgtgg ccccaactga ccggcaaagc aacgttgcgc ccagggcagt ggagtaacga
    21541 cgtaccggcg ttgcgcgaaa tattgcaacg cacaggcatg ttggacgggg ggccgaaaat
    21601 tactctacct ggcgatgaca cgccaactga cgcggtagtc agcccatccg ctgttactgt
    21661 tgaaacagca gaaactaagc cgatggacaa gcaaacgacg tctcgtagta aacctgcgcc
    21721 tgccgttcgt gccgcctacg ataatgaact ggtggaagcc gttaaacgtt ttcaggcatg
    21781 gcaaggattg ggggcagatg gtgctattgg cccggcaaca cgtgactggt taaacgtaac
    21841 gcccgcccag cgtgctggcg tgttggctct caacatccag cgattgcgct tgctgccaac
    21901 agagctttct accgggatca tggttaacat tccggcctat tcgctggtct actatcagaa
    21961 cggcaatcag gtgctggatt cgcgagtcat tgtcggtcgc cccgatcgca aaacgccgat
    22021 gatgagcagt gcccttaaca acgtagtggt aaacccgccg tggaacgtac ctccaactct
    22081 ggcacgcaaa gatattctgc caaaagtgcg caacgatccg ggatatctcg aaagccatgg
    22141 ctatacggtg atgcgcggct ggaacagcag agaagcgatt gacccatggc aggttgactg
    22201 gtctacaatc acggcctcga atttaccgtt ccgcttccag caggctccag gcccacggaa
    22261 ctcgctgggg cgctataaat tcaatatgcc gagttcagag gccatttatt tgcatgacac
    22321 gccgaaccac aatctgttca agcgtgatac acgcgcattg agctcaggct gtgtacgagt
    22381 gaataaagct tccgatctgg cgaatatgct gttgcaggat gcaggctgga atgacaaacg
    22441 tatttctgat gcgctgaagc agggtgatac acgttacgtc aatattcggc agtcgattcc
    22501 ggtgaatctc tactacctga cggcctttgt tggtgcagat ggtcgtaccc agtatcgtac
    22561 agatatttac aattatgatc tgcctgcgcg atccagctcg caaatcgtat cgaaagcgga
    22621 acaattaatc aggtaaatga agtagttcgg ggaaatcagt tgtcgtaata attagcatga
    22681 ttgggggcga ttctctgcag cccccgtcac tgctggggtt gagtcatctt gacgtctgct
    22741 ttacgggcgg ttaaggtgcc tcttgtgcgc cagaagtgca tataaacgat aacattgacc
    22801 tgtagacttg attatcatgg acaaattcga cgctaatcgc cgcaaattgc tggcgcttgg
    22861 tggcgttgca ctcggtgccg ccatcctgcc gacccctgcg tttgcaacac tctctacccc
    22921 acgcccgcgc attttgacac tcaataatct tcataccgga gagtcaatca aagcggagtt
    22981 tttcgatggc agaggctata ttcaggaaga attggcaaaa cttaaccatt ttttccgcga
    23041 ttaccgcgcg aacaaaataa agtccatcga cccaggatta ttcgaccagt tgtatcgcct
    23101 gcaagggttg ttaggcacgc gcaaaccggt gcaactcatt tccggttatc gttctattga
    23161 taccaacaat gaactacgcg cccgcagccg tggagtagcg aagaaaagct atcacactaa
    23221 aggccaggcg atggatttcc atattgaagg tatcgcgtta agcaatattc gcaaagccgc
    23281 gttatctatg cgcgcaggtg gtgtaggata ttatccacgt agtaactttg tgcatattga
    23341 taccgggcca gcacggcact ggtagtaatt gcttaacgaa acaggggcag tatgaactat
    23401 cgtattattc cggtcaccgc attctcccag aactgttcat taatctggtg tgaacaaacc
    23461 cgtctggccg cactggtcga tcctggcggc gatgcggaaa aaatcaaaca ggaagttgat
    23521 gacagcggcc tgacactgat gcagatcctg ctgacgcatg gtcatctgga ccacgttggc
    23581 gcagcggcgg aactggcgca acattacggc gtgccggttt ttggcccgga aaaagaagat
    23641 gagttctggc tgcaaggctt gcctgcgcaa agtcgtatgt ttggtctgga agagtgccag
    23701 ccgctgacgc cagatcgttg gctgaacgaa ggcgatacca tcagcatagg gaatgtgact
    23761 ttacaggtgt tacattgccc tgggcatacg ccgggtcatg tcgtgttttt tgatgatcgg
    23821 gcaaagctgc tgatttctgg cgatgttatt ttcaaaggcg gagtagggcg cagtgacttc
    23881 ccgcgtggcg atcataatca actgatttct tcaatcaaag ataaattgct gccactgggg
    23941 gatgacgtga catttattcc gggtcacgga ccattatcca cacttggtta tgaacgcctg
    24001 cataatccct tcctgcaaga cgaaatgccc gtctggtaag gcacataaaa aagcccgctt
    24061 ttaatgcggg cctggatttc tggcaaagtg cgctttgttt atgccggatg cggcacgagc
    24121 gccttatccg gcctacaaaa tcgtgcaaat tcaaaatatt gcaggggacg cgtaggcctg
    24181 ataagcgtag cgcatcaggc aatgttgcgt ttgtcatcag tctcagcccg cttttcagcg
    24241 ggcttcattg tttttaatgc ttacagcact gccacaatcg cttcgcacag cggagccatg
    24301 ttatctggtg tcatcccggc cacatttacg cgaccagaag caaccgcata tacgccaaac
    24361 tcttcgcgca gacgcagcac ttgttctttt gtcaggccac tgaaggagaa catgccgttc
    24421 tgtttgatga taaagctgaa gtcgcggttt gcgccttttt cctgcagcgt attgacgaac
    24481 aactgacgca tacgctgaat acgctggcgc atatcagtca gctcttgttc ccaaatcgca
    24541 cgtaacgcat cgttgctcag gatggtggca acaacagaag cgccgtgtgc tggtgggtta
    24601 gagtagttag cgcgaatcgc cgctttcatt tggctgaatg cgcgatcaac ggtttcactg
    24661 tcggcagcaa ccagagtaca agcgccaaca cgctcgttgt acaggccaaa gtttttagag
    24721 taggaactgg caacaatcag ctctttatgc atagccgcga aagcgcgcag tccttcagca
    24781 tcttcttcca gaccacgggc aaaaccctgg taagcgaagt caaacagcgg taaccagcct
    24841 ttctcaactg agagttgtgc cagtgtctgc cattgttcca gcgtagggtc gataccggtt
    24901 gggttatggc agcagccatg gaacagcact acgtcgccag cctgagcttc attcaggctg
    24961 ttaatcagtg catcgaagtc aagagtgtga ttttccgcat cataataagc gtattcacga
    25021 acttccagac ctgcagagtt aaagacgctc ttatggttcg gccagcttgg gttgctcacc
    25081 cacactcgct taacgctggt attttttgcc aggaaatcgg cagctacgcg tagtgcgcca
    25141 gtgccccccg gagtctgtgc cgtgcgagca cgtttgtcat tgatcagggc gctaccttta
    25201 ccaaacagca gttcctgagt gcagcgacca aattcaggga tgccgtcaat gccgaggtaa
    25261 tttttggtgg tttcattttc gagcagatac tgttcagcct ttttcacgct ggtcagtacc
    25321 ggggttttgc ccgtctcatc tttatagaca ccaatcccga ggttaatttt gccgggacgt
    25381 tcatcggcac gaaacagatc ggccaggccc agaatcgggt cggcaggagc ggcggtaatg
    25441 ttctcaaaca tgacgaggtt ccattatggt tacagaaggg aagtccgcta tcagggtaac
    25501 gggagattta caaaattcca actattactg atgaaaacgc aggctgtttt tgcaagacgt
    25561 gagattgctc tggaaggtat aaaaaaaaca ggaccaaagt cctgtttttt cggcatttaa
    25621 caaagaggta tgctattaga actggtaaac gatacccaca gcaacggtgt cgtctgaacc
    25681 tacgcccagt ttgttgtcag aatcgatctg gttgatgatg tagtcaacat aggtggacat
    25741 gtttttgttg aagtagtagg ttgcgcccac ttcaaagtag ttcaccagat caacatcacc
    25801 gataccttct acgtctttcg ctttagattt ggtgtaagcg atggacggac gcagaccgaa
    25861 atcgaactgg tattgcgcaa ctaacagaac gtcttgcgtt ttgttggcga agccgctggt
    25921 gtttgtaaat ttattagtga tcggcgtagc gttacgggtt tcaccgtagt tcgctgccag
    25981 gtagatgttg ttcgcgtcgt acttcagacc agtagcccac tgttcagctt ttttaccgtt
    26041 gccaagaggt tgagcttctt gcaggttggt acggtcagct gcaccataag caccaacgat
    26101 accaaagcct tcgtattcgt agctgataga accgccaaca ccgtcgccgt tagaacggcg
    26161 tgcagtgtca cgctcgtttt tacccaggta ctgaacagcg aagttcaggc catcaaccag
    26221 accaaagaag ttggagttac gataggtagc aacgccgcca acacgaccaa cgaagaagtc
    26281 atcgctgtat gcagtatcac caccaaattc tggcagcata tcggtgtaac ccagtgcatc
    26341 ataaaccaca ccgtagttac ggccgtaatc gaaagaacca acgtcagcgt atttaagacc
    26401 cgcgaatgcc agacgcgttt tgttaccagt ttgagcgtca gcgccttcag agttgttacc
    26461 ctggaagtta tattcccact gaccataacc ggtcagatcg gaattgattt gagtttcccc
    26521 tttaaaacca agacgggcat aggtcatgtc gccattgcca ccgtaactgt tttcaccgtt
    26581 acccttggaa aaataatgca gaccaacagc tttaccgtac agatctactt tgttgccatc
    26641 tttgttatag atttctgcag cgtttgcagt acctgctact aacagagcag ggacgatcac
    26701 tgccagaata ttgcgcttca tcattattta ttaccctcat ggtttttttt atgacacctg
    26761 ccactgccgt caataagttc tgtcaataaa aatttacgga actattgatg agagtttggt
    26821 gtctttatgt gtctgcaggc atctttccat tcaaactaac gtttcgctac cgtgaaagtg
    26881 ctacaaagat aaagatttag tttcaaaaag aaaaaatatg taaccaaaag taaaaatcaa
    26941 ggaactttgt gaacaccgtc atatttccat agaaacgtga tgatatttac agcaattttt
    27001 atctatttat atgatttact tatattt
//
LOCUS       DAZFQD010000062        26838 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.062, whole genome shotgun sequence.
ACCESSION   DAZFQD010000062 DAZFQD010000000
VERSION     DAZFQD010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26838
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.062"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(7..1214)
                     /gene="rfaL"
                     /locus_tag="ACHKTW_002639"
                     /pseudo
     CDS             complement(7..1214)
                     /gene="rfaL"
                     /locus_tag="ACHKTW_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312527.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase RfaL"
     gene            complement(1211..2203)
                     /gene="rfaC"
                     /locus_tag="ACHKTW_002640"
     CDS             complement(1211..2203)
                     /gene="rfaC"
                     /locus_tag="ACHKTW_002640"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HFO6463639.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAILGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGLDWQTAREPLASLFYNCKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQVECRSTSMSLADLPAQTVFQNLNLEIITNKLTSEIR"
     gene            complement(2207..3253)
                     /gene="rfaF"
                     /locus_tag="ACHKTW_002641"
     CDS             complement(2207..3253)
                     /gene="rfaF"
                     /locus_tag="ACHKTW_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HFO6463640.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYIALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            complement(3263..4195)
                     /gene="rfaD"
                     /locus_tag="ACHKTW_002642"
     CDS             complement(3263..4195)
                     /gene="rfaD"
                     /locus_tag="ACHKTW_002642"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HFO6463641.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LNIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            4499..5357
                     /gene="yibB"
                     /locus_tag="ACHKTW_002643"
                     /pseudo
     CDS             4499..5357
                     /gene="yibB"
                     /locus_tag="ACHKTW_002643"
                     /inference="COORDINATES: protein motif:HMM:NF021152.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibB"
     gene            5632..6828
                     /gene="kbl"
                     /locus_tag="ACHKTW_002644"
     CDS             5632..6828
                     /gene="kbl"
                     /locus_tag="ACHKTW_002644"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HFO6463642.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPYLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            6838..7863
                     /gene="tdh"
                     /locus_tag="ACHKTW_002645"
     CDS             6838..7863
                     /gene="tdh"
                     /locus_tag="ACHKTW_002645"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HFO6463643.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            8102..9136
                     /gene="waaH"
                     /locus_tag="ACHKTW_002646"
     CDS             8102..9136
                     /gene="waaH"
                     /locus_tag="ACHKTW_002646"
                     /EC_number="2.4.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronate:LPS(HepIII) glycosyltransferase"
                     /protein_id="HFO6463644.1"
                     /translation="MMNSTNKLSVIIPLYNAGDDFRTCMESLITQTWTALEIIIINDG
                     STDNSVEIAKHYAENYPHVRLLHQANAGASVARNRGIEVATGKYVAFVDADDEVYPTM
                     YETLMTMALEDDLDVAQCNADWCFRETGETWQSIPSDRLRSTGVLTGPDWLRMGLSSR
                     RWTHVVWMGVYRRDVIVKNNIKFIAGLHHQDIVWTTEFMFNALRARYTEQSLYKYYLH
                     NTSVSRLHRQGNKNLNYQRHYIKITRLLEKLNRNYADKITIYPKFHQQITYEALRVCH
                     AVRKEPDILTRQRMIAEIFTSGMYKRLITNVRSVKVGYQALLWSFRLWQWRDKTRSHH
                     RITRSAFNLR"
     gene            complement(9123..10082)
                     /locus_tag="ACHKTW_002647"
     CDS             complement(9123..10082)
                     /locus_tag="ACHKTW_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HFO6463645.1"
                     /translation="MFPFRRNVLAFAALLALSSPVLAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNAVPDAPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKFNASAN"
     gene            complement(10086..11345)
                     /gene="envC"
                     /locus_tag="ACHKTW_002648"
     CDS             complement(10086..11345)
                     /gene="envC"
                     /locus_tag="ACHKTW_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418070.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HFO6463646.1"
                     /translation="MTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADERDQLKSIQ
                     ADIAAKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNKQIDEMNAS
                     IAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYFGYLNQARQ
                     ETIAQLKQTREEVAMQRAELEEKQSEQQTLLYEQRAQQAKLTQALNERKKTLAGLESS
                     IQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRDRQKEATRKGTTYK
                     PTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIGASEGTEVK
                     AIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQPIALVGSS
                     GGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            complement(11379..12923)
                     /gene="gpmM"
                     /locus_tag="ACHKTW_002649"
                     /gene_synonym="pgmI"
     CDS             complement(11379..12923)
                     /gene="gpmM"
                     /locus_tag="ACHKTW_002649"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HFO6463647.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLTGA
                     VDKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVEGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            13167..13598
                     /locus_tag="ACHKTW_002650"
     CDS             13167..13598
                     /locus_tag="ACHKTW_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFO6463648.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVISRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKPVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            13740..13991
                     /gene="grxC"
                     /locus_tag="ACHKTW_002651"
     CDS             13740..13991
                     /gene="grxC"
                     /locus_tag="ACHKTW_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HFO6463649.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGRTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            14054..14521
                     /gene="secB"
                     /locus_tag="ACHKTW_002652"
     CDS             14054..14521
                     /gene="secB"
                     /locus_tag="ACHKTW_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HFO6463650.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            14521..15540
                     /gene="gpsA"
                     /locus_tag="ACHKTW_002653"
     CDS             14521..15540
                     /gene="gpsA"
                     /locus_tag="ACHKTW_002653"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFO6463651.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            15620..16441
                     /gene="cysE"
                     /locus_tag="ACHKTW_002654"
     CDS             15620..16441
                     /gene="cysE"
                     /locus_tag="ACHKTW_002654"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HFO6463652.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(16494..16967)
                     /gene="trmL"
                     /locus_tag="ACHKTW_002655"
     CDS             complement(16494..16967)
                     /gene="trmL"
                     /locus_tag="ACHKTW_002655"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFO6463653.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDFRAFLEAENPQRLFALTTKGTPAHSAVNYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
     gene            complement(17122..18312)
                     /gene="lldD"
                     /locus_tag="ACHKTW_002656"
     CDS             complement(17122..18312)
                     /gene="lldD"
                     /locus_tag="ACHKTW_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HFO6463654.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKEFPAALAPMAK
                     GNAA"
     gene            complement(18309..19085)
                     /gene="lldR"
                     /locus_tag="ACHKTW_002657"
     CDS             complement(18309..19085)
                     /gene="lldR"
                     /locus_tag="ACHKTW_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HFO6463655.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            complement(19085..20740)
                     /gene="lldP"
                     /locus_tag="ACHKTW_002658"
     CDS             complement(19085..20740)
                     /gene="lldP"
                     /locus_tag="ACHKTW_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HFO6463656.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLDKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            complement(21108..25958)
                     /gene="ehaG"
                     /locus_tag="ACHKTW_002659"
     CDS             complement(21108..25958)
                     /gene="ehaG"
                     /locus_tag="ACHKTW_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric autotransporter adhesin EhaG"
                     /protein_id="HFO6463657.1"
                     /translation="MNKIFKVIWNPATGNYTVTSETAKSRGKKSGRSKLLISALVAGG
                     MLSSFGALANAGNDNGQGVDYGSGSAGDGWVAIGKGAKANTFMNTSGSSTAVGYDAIA
                     EGQYSSAIGSKTHAIGGASMAFGVSAISEGDRSIALGASSYSLGQYSMALGRYSKALG
                     KLSIAMGDSSKAEGANAIALGNATKATEIMSIALGDTANASKAYSMALGASSVASEEN
                     AIALGRSSVASGTDSLAFGRQSLASAANAIAIGAETEAAENATAIGNNAKAKGTNSMA
                     MGFGSLADKVNTIALGNGSQALADNAIAIGQGNKADGVDAIALGNGSQSRGLNTIALG
                     TASNATGDKSLALGSNSSANGINSVALGADSIADLDNTVSVGNSSLKRKIVNVKNGAI
                     KSDSYDAINGSQLYAISDSVAKRLGGGAAVDVDDGTVTAPTYNLKNGSKNNVGAALAV
                     LDENTLQWDQTKGKYSAAHGTSSPTASVITDVADGTISASSKDAVNGSQLKATNDDVE
                     ANTANIATNTSNIATNTANIATNTTNITNLTDSVGDLQADALLWNETKKAFSAAHGQD
                     TTSKITNVKDADLTADSTDAVNGSQLKTTNDAVATNTTNIANNTSNIATNTTNISNLT
                     ETVTNLGEDALKWDKDNGVFTAAHGTETTSKITNVKDGDLTTGSTDAVNGSQLKTTND
                     AVATNTTNIATNTTNISNLTETVTNLGEDALKWDKDNGVFTAAHGNNTASKITNILDG
                     TVTATSSDAINGSQLYDLSSNIATYFGGNASVNTDGVFTGPTYKIGETNYYNVGDALA
                     AINSSFSTSLGDALLWDATAGKFSAKHGTNGDASVITDVADGEISDSSSDAVNGSQLH
                     GVSSYVVDALGGGAEVNADGTITAPTYTIANADYDNVGDALNAIDTTLDDALLWDADA
                     GENGAFSAAHGKDKTASVITNVANGAISAASSDAINGSQLYTTNKYIADALGGDAEVN
                     ADGTITAPTYTIANAEYNNVGDALDALDDNALLWDETANGGAGAYNASHDGKASIITN
                     VANGSISEDSTDAVNGSQLNATNMMIEQNTQIINQLAGNTDATYIQENGAGINYVRTN
                     DDGLAFNDASAQGVGATAIGYNSVAKGDSSVAIGQGSYSDVDTGIALGSSSVSSRVIA
                     KGSRDTSITENGVVIGYDTTDGELLGALSIGDDGKYRQIINVADGSEAHDAVTVRQLQ
                     NAIGAVATTPTKYFHANSTEEDSLAVGTDSLAMGAKTIVNGDKGIGIGYGAYVDANAL
                     NGIAIGSNAQVIHVNSIAIGNGSTTTRGAQTNYTAYNMDAPQNSVGEFSVGSADGQRQ
                     ITNVAAGSADTDAVNVGQLKVTDAQVSQNTQSITNLDNRVTNLDSRVTNIENGIGDIV
                     TTGSTKYFKTNTDGVDASAQGKDSVAIGSGSIAAADNSVALGTGSVATEENTISVGSS
                     TNQRRITNVAAGKNDTDAVNVAQLKSSEAGGVRYDTKADGSIDYSNITLGGGNGGTTR
                     ISNVSAGVNNNDAVNYAQLKQSVQETKQYTDQRMVEMDNKLSKTESKLSGGIASAMAM
                     TGLPQAYTPGASMASIGGGTYNGESAVALGVSMVSANGRWVYKLQGSTNSQGEYSAAL
                     GAGIQW"
     gene            complement(26002..26685)
                     /locus_tag="ACHKTW_002660"
     CDS             complement(26002..26685)
                     /locus_tag="ACHKTW_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HFO6463658.1"
                     /translation="MNLKKIFFSAVTVSVLCALTGCDYIEEGKPESSLLKQQEEHNNK
                     IDLLEKQQAQLKSQLETIQKQQTGIISSTKTLTHVIKSVKDQQNTFIFTEFNPAKTKY
                     FILNNGSVALAGRVLSIDATENGSVIHISLVNLLSTPISNIGFNATWGGEKPVDAKEF
                     ARWQQLLFNTSMKSTLKLLPGQWQDINLTLKGVSPNNLGYLKLAINMENIQFDNLPSA
                     ENRQKRSKK"
BASE COUNT         6442 a         6744 c         6747 g         6905 t
ORIGIN      
        1 tattttttac ttgtttttca tcgctaataa taagccggcg taaacgccta ataaatttgg
       61 ttcaatttgt tctacgtttc cacggataac aaaataacct ataaaagata gtaagattat
      121 ataatatgca ttatatggtg agcgatattt attcttgacg ccatttttta tacactcttt
      181 caatattgca cagtatagca tcataagact taccagccct aataaaccag tgccaaacca
      241 gatgaatagc gcaaaattat gtggccctat tgattgtcta aaggtccatt ctggataatc
      301 aatgacacgt ttattataga catctttata tgcaacgtta ccgtaaccat aaccaataac
      361 aggattttct aatattagat cgagtgcgct gccttgagta ccatttgcat agcgataaga
      421 actattagtt tgttgaagtt tatacgttag ctttgcggtc atctccttat gtgtgaaaat
      481 aaccgacaat gcaatgatgg caaccattac tcctactaat agtaacttcc attgtttaaa
      541 catcagaatc catattaatc caatgactaa cacggaaagc caggcccctc tggataaagt
      601 tcctaatatc agaaaaataa aaataacgct tagaaccaca aaagaaatgc ggtattttgc
      661 tgatttgata agccagagat ttaacaatgc agggaataaa aagaccatcg agtcagaaat
      721 gctacggtgt ctataatctg cgaatggcat tatcccttgt tgatagtcct tataataggt
      781 aatcaattca gaaaaacagc gtaagcccaa cgcactaata aatgagaaga aaacgatttt
      841 ttcgacatct tctcttttct cgtctctcaa tatgatcggt attgctattg tgcatagtaa
      901 agtattctca ataatagctt tgaaaatttc cttcatgcta gcacctgcat caggggattg
      961 aagaagtgac agcaatgcag caatagttaa tatggcaaca ctaccaaaaa gaaatgtttt
     1021 aaatagtgag agatagtgtt ttggccgttt gcagagatag acgattgtcg ttattgtcat
     1081 aagaataaca ataagatgtt tataccttgt aatcccaccc aaaaaatagg tggtaatgaa
     1141 taagaatacg agagctctgt tccagtacgc tatccagttt ttttctcgag agagaaaaat
     1201 aatgttgagg tcatcttatc tccgatgtca acttattggt tattatttca agatttaaat
     1261 tttgaaaaac ggtttgagct ggcaaatctg caagagacat acttgtagaa cgacactcta
     1321 cttgattctt cccataccca ccaattaatc ccggatcggt tggtccataa accgtgatat
     1381 tgggtctatc cagtgccgcc gttaaatggc ttaaccccgt atccaccgac actacaaatt
     1441 tagccccggc cagcacgcgg gcaacgcctt ccagactcat cttcggcaat acttcaacat
     1501 aagcaaatcc ttccgccagt cgtttcgccc gttcttcctc atgcggcgcg ccccacggaa
     1561 gtttaatccg tattcctgaa tcagccagta aaccaatcaa ttctcgccag tgttcttccg
     1621 gccagtgttt atcatcacgg gtcgtcgcat gaagaaatac ggcatattcg ccagcatctg
     1681 taggcaggtt cgtcagaaaa tgctgtgcga tagcataatc gccctgggtt tgcggtttgc
     1741 tatagcccaa acttttggca aacagttcgc gggtgcgttc tacggcgtgc tgctgttttg
     1801 caatatgatg cttacaattg taaaacaggc tggctaaagg ttcgcgagcg gtttgccagt
     1861 ccaatccatg ctttacgcca tgcgccagac gtgtcaccag tgccgcgctt tttaccagcc
     1921 cctgagcgtc gataactgcg tcatagttct ctgcttgtag cgcttcacga aacgctttgc
     1981 gttcagcttt tatgggggcc gagaaccagg ctttacgcca gcgacgtatt gccacaggaa
     2041 taactcgctc aacggcagcg tgccaggaag gaatctgtgc gaacccttct tccaccaccc
     2101 agtcaaactt aatccctagg attgcctgct gggcatcagt gagtgcgggc aacgtatgga
     2161 gaacatcgcc catcgacgat gttttaacga tcaaaacccg catccgtcag gcttcctctt
     2221 gtaacaatag cgcgttgagt tcttccagta cgcgctgggg agtaatgtcg atcaagctct
     2281 ggtgataacc ctccgcagcg tcacctttac gcactttgtg atagccggta atcagacgga
     2341 tcacgcgcgc tttatgggat agcggcggtg tgaagtccgg gctacttgga ccatacaggg
     2401 caaccagcgg acgattgagc gccgccgcaa cgtgcatcag gccagaatcg ttagtgacaa
     2461 tggctttaca ggctgcaatc aggataaccg cttgatcaag ctgtgtttcc cccgccaggt
     2521 tccgacacca tgcctgctgc tcggtattta aagcggcaag aatctcattg cctgcttcat
     2581 gatctttcgc cgagccaaac agaaccacct gataaccttc atcaatcagc tgctttgcca
     2641 gctccgcata gtggtagtgt ggccagcgtt ttgccggacc aaactccgca cccgggcaaa
     2701 aaccaatcat cggacgttct gatgaaagcg aaaattgatt acaggtatat gatttttcac
     2761 cttcgctcac ctgcaactgc ggccataaca atggctgcgg cagatcttgt gctgtgcgca
     2821 taatgccttt gtcataggcc agcgctatat agcgttccac cattagcggc caggcttctt
     2881 tatcgagcac gcgtacatcg ttgagtaaac cgtagcgcat ctcgccgcgc cagccggtgc
     2941 gatgaggaat acccgcgaag aaaggcacta atgcggattt gaaggagtta ggtaagacgt
     3001 aggcgcggtc gtagcgcttt tcacgcaggc tatgacccag tttgcggcgt tcgccgattt
     3061 ccagcgctcc gtgaccgaga ggcataggga tagcttcgtt aacttccggc atccgcgata
     3121 ataatggacg gcaccatgcc ggtgccatca catcgattat cgcctgggga tagcgcgcct
     3181 ggagcgtgcg atagagactt tgcgacatca tcatgtcgcc aacccaagac gggccgatca
     3241 ccagtatttt catgcagagc tcttatgcgt cgcgattcag ccaggccatg tattccgtta
     3301 caccttcagc aacggttttg aacggtttgt cgtaacccgc cgcgcgcaga tttgtcagat
     3361 ctgcctgagt gaacgcctgg tagcggcctt tcagcttatc cgggaacgga atgtattcga
     3421 tctggccttt cttgtgataa gccagcgtag catcagctac agcctggaag gattccgcac
     3481 gaccggtgcc gaggttgaag atgccggaaa cgccattttc caggaaccac aggtttacat
     3541 ctgccacatc gccaacatag acgaagtcgc gtttaaagtt ctcgctacct tcgaacagct
     3601 tcggtgattc accgttatta agctgggtgt tgagatggaa agcgacgctc gccatgctgc
     3661 ctttatggcc ttcacgcggt ccataaacgt tgaaatagcg gaagccaaca atctgcgagt
     3721 tcgcttccgg caggatttgg cgaacatatt catcaaacag gaattttgag taaccgtaga
     3781 cgttcaacgg tttttcgtac tcgcgggatt caataaagtc ggaggtgcgt ccgccgtagg
     3841 tggctgcgga agaagcgtac aggaacggga tttcacgctc caggcagtag tgcagcagct
     3901 ccttggagta ttgatagttg ttatccatca tatacttgcc gtcccactcg gtggtggaag
     3961 agcacgcgcc ttcgtggaaa atcgcttcga catcgccgaa ctcttcgcca gccataatct
     4021 ggatcaggaa gtcttcctta tccatatagt ctgcgatatt cagatccacc aggttcacaa
     4081 acttggtgcc gtctttcagg ttgtccacca ccagaatatc ggtgatgcct ttatcattca
     4141 gggctttaac gatgttgctg ccgataaagc ccgcgccgcc ggtaacgatg atcataactg
     4201 taaccttcga attatggagt cagagacaat ctcagacacg aatgtctgta atcataccat
     4261 cacatccgaa tcccttcagc cctttacata gaggtgcaag gatgctaatt gcaacaaact
     4321 agtttgggtt ttagctagtc cttcatggcg gatgttggtt ttgcatgtga atagtgcttt
     4381 agtatcaata ttccgatgta tgataaataa cggaagcatt tgaatggata atatacagta
     4441 tacattctgt taatatttat gatataattc tttttttata ttaatggtct tattagtaat
     4501 gaaatcatcc accacaatta tcactgcata ttttgacatt ggtagagggg attggactgc
     4561 taataaaggg ttccgtgaaa aactagctcg ttcagttgat gtttatttta gttactttga
     4621 gcgtctggcc gcacttgaaa atgagatgat tattttcact tctcccgatt tgaaaccaag
     4681 ggttgaagcg attcgcaacg gaaaaccaac aacggttatc gttatcgata taaaaaagaa
     4741 atttagatat atcagaagtc gtatcgaaaa aattcaaaaa agatgagtcg tttacaaata
     4801 gacttgagcc tcgacaatta aaaaacccag agtactggtc accagagtat gttttggtat
     4861 gtaaccttaa ggcctatttt gtaaataaag ctatcaacat gggcttagtg aaaacgcctc
     4921 ttgttgcgtg gatagatttt ggctattgcc gtaagccaaa tgtaactcga ggattgaaaa
     4981 tttgggattt cccatttgat gaaagtaaga tgcatctttt cactataaaa aaagggctta
     5041 ctgtcacatc tcaacaacag gtttttgatt tcatgattgg caatcatgta tatattattg
     5101 ggggcgcaat tgttggttca cagcataaat ggaaagaatt ctataaatta gttctggaaa
     5161 gtcaaaaaat aacactaaat aataacattg tggatgatga ccagggcatt tttgtcatgt
     5221 gcgattacaa acgccccgat ctcttcaacc taaactatct ggggagagga aaatggttcg
     5281 atttgtttcg ctgcttcagg agtaacactt taggggcaaa aatgcaggcg ctgagaattt
     5341 tcctttcaag aaaataggtt tccctccatt agtgggtagc gttttgatac caactgagat
     5401 aggaaaaatg atgctttggc atcatttttt ctttttatat caatgcagta aaatcaatca
     5461 tcattatgcc cagccaaaac tgtacagttt ttctactatc tgtaggtgtt gagagacatc
     5521 aagttacacg ttatttatcc tgaattttgc agaagtgtta acgcgtcatc tcgtcgcgac
     5581 ctataagttt gggtaatatg tgctggaatt tgccctgtct ggagaatcgc aatgcgtgga
     5641 gaattttatc agcagttaac caacgatctg gaaaccgcac gggcggaagg gttgtttaaa
     5701 gaagagcgca ttattacgtc tgcgcagcaa gcagatatca ctgtggctga tggaagccac
     5761 gtcattaact tttgtgccaa taactatctc gggctggcga atcatccgta tctgattgcg
     5821 gcggcaaagg cgggaatgga ttctcacggt ttcggcatgg cttcggtgcg ttttatttgc
     5881 ggcactcagg acagccataa agagcttgaa caaaaactgg cggcattcct ggggatggaa
     5941 gatgcgattc tctactcttc ctgctttgat gctaacggtg gcctgtttga aacgctgctg
     6001 ggcgcggaag acgccattat ctccgacgca ctgaaccacg cgtctattat tgatggtgtg
     6061 cgtctgtgta aagctaaacg ctatcgctat gccaacaacg atatgcagga gcttgaagct
     6121 cgtctaaaag aagcgcgtga agccggtgcg cgtcatgtgc tgattgccac cgatggtgtg
     6181 ttctcgatgg acggcgtgat tgccaacctg aagggcgttt gcgatctggc agataaatac
     6241 gatgccctgg tgatggtaga cgactcccac gcggtcggtt ttgtcggtga aaacggtcgt
     6301 ggttcccatg aatactgcga tgtgatgggc cgggtcgata ttatcaccgg cacacttggt
     6361 aaagcgctgg gcggggcttc tggtggttat accgcggcgc gcaaagaggt ggttgagtgg
     6421 ctgcgccagc gttctcgtcc gtacctgttc tccaactcgc tggcaccggc cattgttgcc
     6481 gcgtccatca aagtactgga gatggtcgaa gcgggcagcg aactgcgtga ccgtctgtgg
     6541 gcgaacgcgc gccagttccg tgagcaaatg tcggcggcgg gctttaccct ggcgggagcc
     6601 gatcatgcca ttattccggt catgcttggc gatgcagtag tggcgcagaa atttgcccgt
     6661 gagctgcaaa aagagggcat ttacgtcacc ggtttcttct atccggtcgt tccgaaaggt
     6721 caggcgcgta ttcgtaccca gatgtctgcg gcgcataccc ctgagcaaat tacgcgtgca
     6781 gtagaagcat tcacgcgtat tggtaaacaa ctgggcgtta tcgcctgagg atgtgagatg
     6841 aaagcgttat ccaaactgaa agcggaagag ggcatctgga tgaccgacgt tcctgtaccg
     6901 gaactcgggc ataacgatct gctgattaaa atccgtaaaa cagccatctg cgggactgac
     6961 gttcacatct ataactggga tgagtggtcg caaaaaacca tcccggtgcc gatggtcgta
     7021 ggccatgaat atgtcggtga agtggtaggt attggtcagg aagtgaaagg cttcaaaatc
     7081 ggcgatcgcg tttctggcga aggccatatc acctgcggtc actgtcgcaa ctgtcgtggc
     7141 ggtcgtaccc atctgtgccg caacacgatc ggcgtcggcg ttaaccgtcc gggctgcttc
     7201 gccgaatatc tggtgatccc ggcgttcaac gccttcaaaa tcccagacaa tatctccgac
     7261 gacctggctt ccatttttga tcccttcggt aacgccgtgc ataccgcgct gtcgttcgat
     7321 ctggtgggcg aagatgtgct ggtttctggt gcaggtccga taggtattat ggctgcggcg
     7381 gtggcgaaac acgttggtgc acgcaatgtg gtgatcaccg atgttaacga ataccgcctt
     7441 gagctggcgc gcaaaatggg tatcacccgt gcggttaacg tcgcgaaaga aaaccttaat
     7501 gatgtgatgg ctgaactggg catgaccgaa ggctttgatg tcggtctgga aatgtccggc
     7561 gcgccgccag cgtttcgtac catgctcgat accatgaacc acggcggtcg tattgcgatg
     7621 ctgggtattc caccgtccga tatgtctatc gactggacca aagtaatctt taaaggcttg
     7681 ttcattaaag gtatttacgg tcgtgagatg tttgaaacct ggtacaagat ggcggcgctg
     7741 attcagtcag gtctggatct ctcgccgatc attacccatc gtttctctat cgatgatttc
     7801 cagaagggct ttgacgctat gcgttcgggc cagtccggga aagtaattct gagttgggat
     7861 taacacgaac aagggctggt attccagccc ttttgcctga ggataatccg ttaaatatgt
     7921 aaaatcctgt cagtgtaata aagatatcgt aattgcgctg atctcttata tagctgctct
     7981 cattatctct ctaccctgaa gtgactctct cacctgtaaa aataatatct cacaggctta
     8041 atagtttctt aatacaaagc ctgtaaaacg tcaggataac ttcagaggtc gtcggtaatt
     8101 tatgatgaac agcaccaata aacttagtgt tattattccg ttatataatg cgggcgatga
     8161 tttccgcact tgtatggaat ctttaattac gcaaacctgg actgctctgg aaatcattat
     8221 tattaacgat ggttcaacgg ataattctgt tgaaatagca aagcattacg cagaaaacta
     8281 tccgcacgtt cgtttgttgc atcaggcgaa tgctggcgca tcggtagcgc gtaatcgtgg
     8341 gatcgaggtg gcgacgggca aatatgtcgc ttttgtcgat gctgacgatg aggtctatcc
     8401 caccatgtac gaaacgctga tgaccatggc gttagaggat gacctcgacg tggcgcagtg
     8461 caacgctgac tggtgttttc gtgaaacggg agaaacctgg caatccatcc ccagcgatcg
     8521 ccttcgctca accggcgtat taaccggccc ggactggctg cggatggggc tttcttcgcg
     8581 ccgttggact cacgttgtct ggatgggggt ttatcgccgt gatgttattg ttaaaaataa
     8641 cattaaattt attgccggat tacatcatca ggatattgtc tggacaacag aattcatgtt
     8701 taacgcgctg cgtgcgcgat ataccgagca atcattatat aaatattatc tgcataatac
     8761 gtcagtgagt cggttgcata gacaagggaa taaaaacctt aattatcaac gtcactatat
     8821 taagattacc cgcctgctgg agaaattaaa tcgaaattat gccgacaaaa ttacgattta
     8881 tccgaaattt catcagcaaa taacttacga agcattgcgt gtttgccatg cggtgcgcaa
     8941 agagccggat attcttaccc gccaacggat gattgccgag atatttactt ccggtatgta
     9001 taagcgcctg attaccaatg tgcgcagcgt gaaggtcggt taccaggcgt tactgtggtc
     9061 tttccgctta tggcaatggc gcgacaaaac gcggtcgcac catcgcatta cgcgtagcgc
     9121 ctttaatttg cgctagcgtt aaacttcgcc gcttcgggct gtttgcccca gccttgccac
     9181 tgatgctgga agtaaacgat cagcgtgctc tggctgatgc tttcgcttaa tacttcaaag
     9241 aagcgattag cataaacagg ttctatcggt ttcttcggtt tgcacagctt cacgccacgg
     9301 aacggattac gcggtgcatc aggcacagcg tttttcggcg gtgtagaagt atcaacctgc
     9361 ggttcattca gcaagctgct ggctttcacc agcgtaatgt ctggcggcag gttataaacc
     9421 atctgttgta gcacgcgtac cgttgaaggg tgaggatgcc caatggcaat ggttgaaccg
     9481 ttgcgacgcg ccagatcaat tgcgcgatta aattgcacac ggatgtccgc ttcgttctgc
     9541 gaatcgtcga ggaacacctt ccgtttgatc accttcacgc cagtaccttg cgcggcgcgc
     9601 atcgcctggg tattaccgat ggttacgctg tcgaggaagt aaagattgta acgctccagt
     9661 gcctgcatca ctttctgcat accaaacagg ttagaggtca tcttgctacc catgtggttg
     9721 ttgatcccca cggcataggg cacgttattg accgcactac gaataatgcg ctcaatttcg
     9781 tcgctgctca tctccgggcg cagcgtattt ttctccagcg gctgtttact cagtggtgcc
     9841 atcgggagat gaatcaacac ttcgtgcccg ctgttatgcg ctttggtcgc catctctctg
     9901 gcgtgcggtg aatcgggtaa tacagcgacg gagatagcgg agggcatcgc cagcacctgg
     9961 ttttcgttgt gcgggcgata cccaaaatca tcaatgacga tggcaagttt gccagcaagt
    10021 acgggggagg agagcgccaa cagagcggca aatgcaagaa cgttacgacg aaatggaaac
    10081 aaaacttatc ttcccaacca cggctgtgga ttgaccgcct gaccctggcg gcgaatttcg
    10141 aaatagagtg aaggccgacc ctgaccgcca ctgctgccca ccagtgcaat cggctggccc
    10201 gcgcgaacct gcgaaccaac gctcaccagc gcgctctgat tatagccgta aagactcatg
    10261 tcgcctttac catgctcaac caccaccacc aagccgtaac cttgcagcca gtcagccaga
    10321 atcacccgac cgtcggcaat cgctttaact tcagtacctt cagaggcacc aatcaccatc
    10381 cctttccagc gtaattcacc ctgtagctgc tcgccatagc gatgcagcgt cggcccgcga
    10441 accggccaga atgcctgacc acgcggcgca cccagaccac cggtacggga catcagcgat
    10501 ttttcgcttt cggtcggttt ataggtggtg cctttgcgcg tcgcttcttt ctggcggtca
    10561 cgaaccgcct gggcctcacg tgcttctcgt tctgcacgcg ctttcgccgc ggcttccgca
    10621 cgggcaatgc tgttacgcag acgggattcg ttggcacgca gttcacttag ctgctgctga
    10681 ccttgctgga tggaagattc cagccccgcc agcgtctttt tacgctcgtt cagcgcctga
    10741 gtcagtttcg cctgttgggc gcgctgctca tataacagcg tttgttgctc gctctgtttc
    10801 tcttccagtt cagcacgctg catggcgact tcttcacgcg tttgcttcaa ctgggcaatg
    10861 gtttcttgtc gcgcctggtt gagatagccg aaataagcct gtaaacgctg tccacgctgg
    10921 ctttcttcac cgctgagaat cagctgaata ccggtatgct cgccctgacg gaatgcggca
    10981 tccagttgtg cggcgaggct gcgctcctgg gcggcttttt gctgctccag tttggcaatc
    11041 gacgcgttca tctcatcaat ctgtttattg agttgattga gcgtgttttg cgtttcgcgc
    11101 agcttacggg tggcttcaga gatcgcttct tcctgctttt tcaattgtgc gagcaggctt
    11161 gcgcgttgtt gttgcttttg gcgtaccgcg cgctctttcg cggcgatatc ggcctgaata
    11221 gatttgagtt ggtcacgctc atccgcgtgg gcggaaaagg cgcacaacaa tacgccagcg
    11281 ctaagaacgc tggcgtagat gatgggcctg attgcaaacc tgcgcggttt cacggcccgt
    11341 gtcatggtat taatcgcctt tcccctcatg gggagggatt attccacgat gaacagcggc
    11401 ttaccagtca tctcttgcgg gatttccata cccatcagcg acaacatggt cggcgcgatg
    11461 tcagaaagtt tgccgccttc aaccgctttc acgttcttat cgccaacgta aatcagcgga
    11521 actggcaggt tggtgtgtgc cgtgtgtgcc tgacccgttg ccggatcgcg catctgttca
    11581 gcgttaccgt ggtcagcggt gatcagcagt tgtccaccca cggactcaac cgctttcgcg
    11641 acttcttcca cgcagtgatc cagcgcttca accgctttaa ccgctgcttc catcaccccg
    11701 gtgtgaccta ccatgtcgcc gttcggatag ttacagataa tggtgtcgta tttgccgctc
    11761 ttgattgccg caaccagttt ttcggtcagc tctgcggagc tcatttccgg ttgcagatcg
    11821 taggtagcca ctttcggtga gttgatcaga atgcgatctt cgcctttgaa cgactcttct
    11881 acgccaccgt tgaagaagaa agtaacgtgg gcatattttt cggtttcgga aatacgcaac
    11941 tgagttttgt cgtttttcgc catccactcg ccgaaggtgt taaccaggga agcgggtggg
    12001 taagcaaccg cagttttgat gtcagcggcg tattcggtca gcatcacgaa atcgacgtta
    12061 accactttct tacgcgcgaa gccatcgaaa tcagcgttca cgaatgcacg agtgatttca
    12121 cgcgcgcggt cagcacggaa gttcatgaaa atcagcgcat cgccgtcttc cattgccgca
    12181 tctggctgac cttctgcacg aataacggtc gctttcacga actcgtcgtt ttcgtcgcga
    12241 gcataagctg cctgcaaacc agcaacggca gtatcggcct ggaactcacc ctgcgccaga
    12301 gtcagcaggt cataggcttt ttcaacgcga tcccaacggt tatcgcggtc catcgcgtag
    12361 taacgaccaa tgatggacgc tacgcggcct ttacccagcg cagcaaattt ctcttcaaat
    12421 tttttcagcg aggattcagc actgcgcggc ggggtgtcgc gaccgtcaag gaatgcgtgc
    12481 aggtagattt tttctgcgcc gcgttcagct gccagttcta ccatcgccat gatgtgatct
    12541 tcgtggctgt gtacgccgcc tgcggagagc agacccataa tgtgtactgc tttacctgcg
    12601 tttttcgctt tatctaccgc gccagtcagc actggattag caaagaaagc gcgatctttg
    12661 atttcaacgt ccagacgagt caggtcctga tacacgatgc ggccggcacc caggttaacg
    12721 tggcctactt cggagttacc catctgacgg tcaggcagac cgacttccag accggaagcg
    12781 tcgatcaggg tatgcggacg attggcccac agtgcatcca ttaccggggt tttagcacta
    12841 aaaatggcgt tatcctgctg ttcttcacga tagccatagc catccagaat caccagtacc
    12901 ataggttttt tagaaaccga cattgcgaca acctcatact caagagtcaa aatttgcgta
    12961 attttactac agctgaatcg ataaaatagc ctctgaagat caaaatatac gaccgctcgc
    13021 ggcaagccga tgccagattt gcgctttttt tcgtcgtgcc ccgcaaaatt gcattccagt
    13081 taacgcgctg gctgtatttg ccgcaccgcg caggtatact cctttcctgg tttttttaat
    13141 cactacgtcg ggagttgtta ccccccatgc aagaaattat gcaatttgtt ggccgtcatc
    13201 ccatactgag tatcgcctgg atcgcgttac tggtggcggt tcttgtgact acgtttaaga
    13261 gcctgacctc gaaagtgaag gtgattagtc gtggtgaagc tacgcgtctg atcaacaaag
    13321 aagacgctgt ggttgtggat ttacgtcagc gtgatgactt ccgtaaaggc catatcgcag
    13381 gttctatcaa tctgttgccg agtgaaatca aagccaacaa tgttggtgag cttgagaagc
    13441 acaaagacaa accggttatc gtggtagacg gttctggcat gcagtgccag gagcctgcaa
    13501 acgcgctgac gaaagctggt tttgcgcaag tattcgtgct gaaagagggc gttgctggct
    13561 gggctggcga aaacttgcct ctggtgcgcg gcaaataatt tacctgtcgt ctttcatgat
    13621 gcaggccgtt agctgcaatt aggcccccag cgacttattg ttataagctt ctggggacac
    13681 tccattttgc agccgccctg caacatgaaa tttcagggta aaaaaacagg agctaaatta
    13741 tggccaatgt tgaaatctat accaaagaaa cctgcccgta ttgccatcgt gcaaaagcac
    13801 tgctgagcag caagggcgtg agttttcagg agttgccgat cgatggcaat gccgccaagc
    13861 gtgaagagat gatcaaacgc agcggtcgca ccacggttcc ccagattttt attgacgcac
    13921 agcacattgg cggctgtgat gacttgtatg cattggatgc acgtggtgga ctggatcccc
    13981 tgctgaaata acgtgtgaac gttggcatta cattgcgcag tatttaagga caacacttaa
    14041 gggttttcta cacatgtcag aacaaaacaa cactgaaatg actttccaga tccaacgtat
    14101 ttataccaag gatatctctt tcgaagcgcc gaacgcgccg cacgttttcc agaaagattg
    14161 gcaaccagaa gttaaacttg atctggatac ggcatcttcc caactggcag atgacgtata
    14221 cgaagtggta ctgcgtgtta ccgtaacggc ctccctgggc gaagaaaccg cgttcctgtg
    14281 tgaagttcag cagggcggta ttttctccat cgcgggtatc gaaggcaccc agatggcaca
    14341 ttgcctgggc gcatactgcc cgaacattct gttcccgtat gctcgtgagt gcatcaccag
    14401 catggtatcc cgcggtacat tcccgcaact gaaccttgcg ccggttaact tcgatgcgct
    14461 gttcatgaac tatttgcagc agcaggctgg cgaaggtact gaagaacatc aggatgcctg
    14521 atgaaccaac gtaatgcttc aatgactgtg atcggtgccg gctcgtacgg caccgctctt
    14581 gccattaccc tggcaagaaa tggccacgag gttgtcctct ggggccatga ccctgaacat
    14641 atcgcaacgc ttgaacgcga ccgctgtaac gccgcgtttc tccccgatgt gccttttccc
    14701 gatacgctcc atcttgaaag cgatctcgcc actgcgctgg cagccagccg taatattctc
    14761 gtcgtcgtac ccagccatgt ctttggtgaa gtgctgcgcc agattaaacc gctgatgcgt
    14821 cctgatgcgc gtctggtgtg ggcgaccaaa gggctggaag cggaaaccgg gcgtctgtta
    14881 caggacgtgg cccgcgaggc gttaggcgat caaattccgc tggcggttat ctctggccca
    14941 acgtttgcga aagaactggc ggcaggttta ccgacagcta tttcgctggc ctcgaccgat
    15001 cagacctttg ccgatgatct ccagcagctg ctgcactgcg gcaaaagttt ccgcgtttac
    15061 agcaatccgg atttcattgg cgtgcagctt ggcggcgcgg tgaaaaacgt cattgccatt
    15121 ggtgcgggga tgtccgacgg tatcggtttt ggtgcgaatg cgcgtacggc gctgatcacc
    15181 cgtgggctgg ctgaaatgtc gcgtcttggc gcggcgctgg gtgccgatcc tgccacgttt
    15241 atgggcatgg ccgggctggg cgatctggtg cttacctgta ccgacaacca gtcgcgtaac
    15301 cgccgttttg gcatgatgct cggtcagggc atggatgtac aaagcgcgca ggagaagatt
    15361 ggtcaggtgg tggaaggcta ccgcaatacg aaagaagtcc gcgaactggc gcatcgcttc
    15421 ggcgttgaaa tgccaataac cgaggaaatt tatcaagtat tatattgcgg aaaaaacgcg
    15481 cgcgaggcag cattgacgtt attaggtcgt gcacgcaagg acgagcgcag cagccactaa
    15541 ccccagggaa cctttgttac cgctatgacc cggcccgcgc agaacgggcc ggtcattatc
    15601 tcatcgtgtg gagtaagcaa tgtcgtgtga agaactggaa attgtctgga acaatattaa
    15661 agccgaagcc agaacgctgg cggactgtga gccaatgctg gccagttttt accacgcgac
    15721 gctactcaag cacgaaaacc ttggtagtgc actgagctac atgctggcga acaagctgtc
    15781 atcgccaatt atgcctgcta ttgctatccg tgaagtggtg gaagaagcct acgccgctga
    15841 cccggaaatg atcgcctctg cggcctgtga tattcaggcg gtgcgtaccc gcgacccggc
    15901 agtcgataaa tactcaaccc cgttgttata cctgaagggt tttcatgcct tgcaggccta
    15961 tcgcatcggt cactggttgt ggaatcaggg gcgtcgcgca ttggcaatct ttctgcaaaa
    16021 ccaggtttct gtgacgttcc aggtcgatat tcacccggca gcaaaaattg gccgtggcat
    16081 tatgctcgac cacgccacgg gcatcgttgt gggtgaaacg gcggtaattg aaaacgacgt
    16141 atcgattctg caatctgtga cgcttggcgg tacgggtaaa tctggtggtg accgtcaccc
    16201 gaaaattcgt gaaggtgtga tgattggcgc gggcgcgaaa atcctcggca atattgaagt
    16261 tggtcgcggc gcgaagattg gcgcaggttc cgtggtgctg caaccggtgc cgccgcatac
    16321 aaccgccgct ggcgttccgg ctcgtattgt cggtaaacca gacagcgata agccatcaat
    16381 ggatatggac cagcatttca acggtattaa ccatacattt gagtatgggg atgggatctg
    16441 atgtgctgtg atcgtgccgg atgcgatgta atcatctatc cggcatacag taactaatct
    16501 ctcaacaacg ctcccggata tcccagctgc ctccaggctt catacaccac aactgacacc
    16561 gcattggaca ggttcatgct gcggctgtcc ggcaccatcg gaatgcgaat tttttgttca
    16621 gcgggcaggg catcaagaat gctcgctggc aggccgcgtg tttccgggcc gaacatcaga
    16681 taatcgccat cctgatagtt tacggcgctg tgagcaggcg tacctttcgt ggtgagggca
    16741 aacaggcgct ggggattttc tgcttcgagg aacgcgcgaa agtcatgatg acgcgtaacg
    16801 gcggtaaact cgtgatagtc caaccccgcg cggcgcaggc gcttatcgtc ccaggcaaat
    16861 cccatcggtt cgatgatatg cagacgaaag ccggtattag cgcaaagacg gatgatgttg
    16921 ccagtatttg gcggaatttc tggttcgtaa agtacgatgt ttagcatact gcccccttag
    16981 tgcgggggca ggatagcaga aaattggtga gcagtgcggc cttttcccct caccccggcc
    17041 ctctccctgt ctcttgatca ggagatctga tcaagagaca gctccctgag ggagagggtt
    17101 agggggaggg ggcgcaaacg actatgccgc attccctttc gccatcggag ccagtgccgc
    17161 aggaaactct ttacccagcc cctgcaccag cgaatcttgc gtaatttcgc tgatcgattt
    17221 cgcgccagtc agcgtcatcg ccactttcat ctctttctcg atcagattta gcaggttagc
    17281 tacacccgcc tggcccgctg ttgccagcgc atacaggaaa gcacgaccca gcagtacggt
    17341 gtcggcaccg agcgcaatca tacgcacgac atcaagcccg ttacgaattc cgctatccgc
    17401 cagaatggcg atatcacctt tcaccgcatc tgcaatagcg ggcaacgcac gagcggaaga
    17461 gagtacaccg tccagctggc ggccaccgtg gttagaaacc acaattccat cagcaccaaa
    17521 acgtactgca tcgcgcgcat cttccggatc gaggatccct ttgatcacca tcgggccatc
    17581 ccagaaatcg cggatccact caaggtcttt ccatgagatg gacggatcga agttattccc
    17641 cagccagccg atgtaatctt ccagtccggt cggtttgccg agataagctg agatattacc
    17701 taaatcatgt ggacgaccgt tcaggcccac atcccacgcc cattgcggat gcgtcaccgc
    17761 ctgcaagtag cggcgcattg ccgcatttgg gccgctcata cctgaatgcg catcacggta
    17821 gcgtgcgccc ggtgtcggca tatccacggt gaaaaccagc gtcgaacaac ccgctgcttt
    17881 tgctcgctcc agcgcgttac gcataaagcc gcgatcgcgc agtacataaa gctggaacca
    17941 cattgggcgc ttgatggctg gcgcgacttc ttcaatcggg caaacggaaa ccgtcgagag
    18001 agtaaacgga atgccatgcg cgtccgccgc tttggctgcc tgaacttcgc cacgacgcgc
    18061 atacatgcca cacaaaccca ccggtgccag tgctaccggc atcgacaatt tctcattaaa
    18121 cagcgtcgtt tccaggctta agtcggacat gtttttcaga atacgctggc gcagtgccac
    18181 ttctgacaaa tcttccacgt tgcggcgcag cgtgtattca gaatatgcac caccatccat
    18241 atagtggaac aggaacggcg gcagaatgcg ttgcgctgcg gcgcgataat cgctggctgc
    18301 ggaaataatc atgcgttttt ctccctcgaa tgctcattat gctcaccggg caggcgggta
    18361 atccgtgcgt ggcgagcctg atcttcatcg aatcgtttca tggtggtgtg aacaaaacta
    18421 aggtgcgcca tcattgcttt acgcgccccg tcggcatcac cagcaaaaat ggcgtcaatg
    18481 acagcctgat gttgttcggt cagttgtgaa aaaaccggtg gcaccagata catccgctga
    18541 cggctatgct tcactgagga ttgcaggaca tcgaagaaac cgcgcatggt ttgcagcagc
    18601 acgatgttat gtgaggcttc ggcaatcgcc agatgaaaac gaacgtccgc ttgtgaggcg
    18661 atatccgggt cttcacttag cgttgcttca aagcaaagct gaatcttttc tttgtcgcca
    18721 ggtgtggcgc gcattgccgc atgccatgcg gtgctggctt caatggcgta gcgggcttcc
    18781 agaatatcga aactgtaatc cggatcatcg gccatcagcg tttttagcgg ctggacgatg
    18841 ttttgctccg accatgtgtc atgacgccag cgaataaacg tcccgccgcc gcgtcgactg
    18901 agcagcacgc cttcgctcac cagttttgcc agcgcctcgc gtagtgaatt acgtgatacg
    18961 ccgagttgca tcgccagttg gcgctcagcg ggcaacttca tgcccgcttc caggtttttt
    19021 tcatcaatca gcgcccgcac acgatcggca acctcgtctg acaggcgtct gggtaaaaca
    19081 atcattaagg aatcatccac gttaagacat aagcctgaag cgtggtgatc acgcccacca
    19141 tacaggtgaa gatcaggctg tgtttgacag taaagcggaa caaatcagac tctttgccca
    19201 ccaggcctac cgccgcacag gcgatagcga tagattgcgg ggagatcatc ttaccggtga
    19261 cgccaccggt ggtattggcg gcaaccagca acagatcaga gacgccaatt tgttgtgctg
    19321 cggtggcttg cagcgcggcg aacagggcgt tagatgaggt atccgacccg gtcaggaata
    19381 cccccagcca gccgaggaac ggcgagaaga aggtgaatgc atgaccggtg tgcgccagtg
    19441 ccagcgccag tgttgatgac agtccggaat agttcgaaat aaaggcgaat gccagcacca
    19501 taccgatgga gtatatgggc agagccagtt ctttcagcgt gctgccgaag gtgctgatag
    19561 cgtcagacgg tttcatcttc agccagacaa tcgagagcag tgcagcaaac agaatggcgg
    19621 tgccggtggc agagaaccag tcaaacttaa acacggcggc ataggctgta gcctcgctga
    19681 ccactggcgg catacgggca accagtttat cgaggtacgg caccggaata ttgatcaccc
    19741 actcatacag cgcgccaccc gatgcgaaca gggctttaaa cggcgggata ctccacagtg
    19801 ttacggtagc tgtcaggaac aggaacggtg tccaggcacg taacacctga cccgcagtgt
    19861 aaccggtgtg ggccagcgtc atatcaacct gtgacgcccc caaatcacca aaacggaata
    19921 cacgcactgg ctgccagcgt ttgaggaaca gcgtcaggca gagcagtgat accagcgaag
    19981 agataatgtc cggcagctcc ggcccaatga agttagagct aaggtactga gcgatggcaa
    20041 acgagccgcc cgcaaccacg accgcaggcc acgtctcttt gataccgcgc cagccgtcca
    20101 taatcgccat gatccagaac agcacgataa tggtcataaa cggtagctgc cgccccacca
    20161 tctgaccaat ctcaaagctg tcgatacctg ttacctgtcc ggcaaccagg attggaatgc
    20221 ccatcgcacc aaatgccact ggcgcggtgt taacaatcag gcacagcccg gcggcgtaca
    20281 gcggtttaaa acccaggccg accagcaatg cggcggtaat tgctaccggt gcgccaaagc
    20341 ctgcggctcc ttcaaggaac gcgccgaaac agaaaccgac gatcagcatt tgcagacgct
    20401 ggtcaggggt tatcgaaaga atagacgagc gaatgatgtc aaactgcccg gttttcaccg
    20461 agatcttata gacgaacacc gctgcaataa tgatccacgc gatgggccac aacccgtaga
    20521 agaaaccata aaccaccgag gccagcgcgt tagcgaccgg cattttatag aacagcaaag
    20581 ccacggcaag ggcgattgcc accgtccacg aggcggcgac gtatcctttc agtttgagct
    20641 taatcagcgc aaagaagaaa aacaggatgg gaagcgatgc tatcagactg gaaagccaga
    20701 tattcccggc gggatcgtag ttttgttgcc agagattcat tgcaggtctc ctggagtcca
    20761 cgcacatcgt gtaatgagtc tgttgctcat ctccttgtca cacgttgtgt aaaagtggca
    20821 ctgccaattg tgttgtgtag ggataatgac aatgaatggt taactaaatg ttatgtattg
    20881 gcaatattga tgtgagtttt aattggggaa ttgtgaacca gaggtcagat aagcgaagaa
    20941 ttggtagggc caattcttgt ggggggagat gtctggtaaa gaaaagggca atcctcatca
    21001 gagaattgcc cttagattgt tgacccggag cgccttatct tgcctgatgc gcgtaacgca
    21061 tcaggcaatt tggcgtttgc ccttagtcac actaacttaa tggatcatta ccactgaata
    21121 ccggcaccga gtgcggcgga gtattcaccc tggctattgg tactaccttg taatttgtag
    21181 acccaacgac cattggcgct caccatcgat acacctaaag caactgccga ttcaccgttg
    21241 taagtaccgc caccaataga agccatgctg gcacccggtg tataagcctg cggcagaccg
    21301 gtcattgcca ttgcagaagc gataccaccg ctcaacttgc tttcagtttt agacagtttg
    21361 ttatccatct caaccattcg ctgatcggtg tattgcttcg tttcctgcac gctttgcttc
    21421 aactgcgcgt aattcaccgc gtcgttgttg ttgacgccag cggagacgtt gctgatacga
    21481 gtcgtaccgc cgttgccgcc accgagggtg atattgctat agtcgataga accatcagct
    21541 ttggtgtcgt aacgcacacc gccagcttcg gaagacttca actgtgccac gttaacagca
    21601 tcggtgtcgt ttttacctgc ggctacgttg gtgatacgac gttgattagt agatgagcct
    21661 acagagatcg tattttcttc ggttgccaca gaccctgtac ccagtgcgac gctgttatcc
    21721 gcggcagcaa tggagccaga accaatcgcg acgctatctt taccctgcgc gctggcatct
    21781 acaccatcgg tattggtctt gaagtacttg gtgctaccgg tggtgacgat atcgccaata
    21841 ccgttttcga tattggtgac gcgtgaatca agattcgtta cccgattatc caggttagta
    21901 atgctctggg tattctggga aacctgcgca tccgttactt tcaactgacc cacgttgacc
    21961 gcatcggtat cagccgaacc tgctgcgacg ttagtgatct gacgttgacc atccgcacta
    22021 ccgactgaga attcaccgac agagttctgc ggtgcgtcca tgttgtaggc ggtataattg
    22081 gtttgagcgc cacgagtggt cgtagaacca ttacctatcg caatactgtt gacatgaatg
    22141 acttgcgcat tgctaccaat ggcaatgccg ttaagtgcat tcgcgtccac gtaggcacca
    22201 taaccgatac caataccttt atcgccattc acgatggttt tcgcacccat tgccagcgag
    22261 tcagttccca ctgccagtga atcttcttcc gttgaattag cgtggaagta tttagtcggc
    22321 gtggttgcga ccgcaccaat cgcattctgc aattgacgaa ccgtaacggc gtcatgggct
    22381 tcggaaccat cggctacgtt gatgatttga cgatatttac cgtcatcacc gatagacaat
    22441 gcaccgagca gttcgccatc cgtggtgtcg taaccaataa caacgccatt ttccgttatg
    22501 ctggtgtcac gggagccttt ggcaatcact cggctggaaa cagagctgct acccagggcg
    22561 atacccgtat caacgtcgct gtagctgccc tgaccaatag ctacgctgct atcgcctttg
    22621 gcgacagagt tataacctat agctgtagcg ccaacaccct gtgcgctggc gtcgttgaac
    22681 gctaagccgt cgtcgttagt acgcacatag ttaatacccg caccgttttc ttggatatag
    22741 gttgcgtcgg tgttaccagc gagctgattg ataatttggg tgttctgctc aatcatcata
    22801 ttcgtcgcat tcaactgaga accgttcact gcatcggtac tgtcctcact aatactgcca
    22861 ttagcgacat tagtgatgat gctggcttta ccgtcatggc tggcattgta ggctccagca
    22921 ccgccattgg cagtctcatc ccacagcagg gcgttatcat caagcgcatc cagggcgtca
    22981 ccgacgttgt tgtactcggc gttcgcaatg gtgtaagtcg gtgcggtgat ggtgccgtca
    23041 gcgttgactt ctgcgtcacc acccagcgca tcagcgatgt acttattggt ggtatagagt
    23101 tgtgagccgt taatcgcgtc gctgctggca gcagagattg caccgttagc gacgttagtg
    23161 attacactgg cagttttatc ttttccgtga gcggcgctaa atgcaccatt ttcaccggcg
    23221 tccgcatccc agagcagagc gtcgtcaaga gtggtatcga tagcattcag ggcatcaccg
    23281 acattatcgt aatcagcatt agcaatggtg tacgtcggcg cagtgatggt gccgtctgca
    23341 ttgacttcgg caccaccccc cagcgcatca acaacataac tgctcacgcc gtggagttgt
    23401 gagccgttta ctgcgtcaga actggagtct gaaatttcac catctgcgac atcagtgatc
    23461 acgcttgcgt caccattagt accgtgtttg gcactgaatt tacctgcggt ggcatcccaa
    23521 agcagagcat cgccgagaga cgtgctaaat gaggagttaa tcgcagccag tgcatcgccg
    23581 acgttataat aatttgtttc accgattttg taggttggac cggtaaacac accgtcagta
    23641 ttcacagaag cattgccgcc gaagtaggtg gcgatattgc tgcttaagtc ataaagctgg
    23701 ctaccgttaa tggcatcgga actggttgca gtgactgtgc cgtccaggat attggtgatt
    23761 ttgctggcgg tattgttgcc atgagctgca gtgaagacac cattgtcctt atcccatttc
    23821 agcgcatcct caccaagatt agtcaccgtc tcagtcagat tagagatgtt ggtggtgtta
    23881 gtggcgatat tggtggtatt cgtcgccacg gcatcgttgg tggttttcag ctgagagccg
    23941 ttaacggcat cggtgctgcc agtcgtcagg tcgccatctt taacgttggt gattttgctg
    24001 gtggtctcgg tgccatgagc tgccgtgaat acaccattgt ccttatccca tttcagcgca
    24061 tcctcaccaa gattagtcac cgtctcagtc aggttagaga tgttggtggt gttagtggca
    24121 atattggaag tgttattggc gatattggtg gtattcgtcg ccacagcatc gttggtggtt
    24181 ttcagctgag agccgttaac agcatcagtg ctgtcagccg tcaggtcggc atctttaacg
    24241 ttggtgattt tgctggtggt atcctggccg tgagctgcac tgaatgcctt tttagtttcg
    24301 ttccagagca gggcatcagc ctgaaggtca ccaacggaat ccgtcaggtt ggtgatattg
    24361 gtggtattgg tggcaatatt tgccgtattc gtggcaatgt tgctggtatt agtagcgata
    24421 ttggcggtgt tggcttcgac atcgtcattg gtagctttca gttgggaacc gttaaccgca
    24481 tccttactgg aggctgaaat cgtgccatcc gcaacatcgg tgattacgct ggcagttggg
    24541 ctactagtac catgagcagc gctgtatttg cctttggttt ggtcccattg cagggtgttt
    24601 tcatcaagta cagcgagcgc agcccctacg ttatttttgc taccattttt taaattgtag
    24661 gttggtgctg taacagtacc gtcatcaaca tctactgcag cccctcctcc aagccttttt
    24721 gctaccgagt cgctaatggc ataaagctgt gaaccattaa tggcatcgta actgtcagac
    24781 ttgatcgcgc catttttcac attaacgatc ttgcgtttta atgaactatt gccgacagag
    24841 acggtattgt ctaaatccgc aatggaatct gcgcccagcg cgacagagtt aataccgttg
    24901 gcactgctat tactaccaag cgcaagactc ttatcaccag ttgcattact ggctgtgcct
    24961 aaggcaatgg tgtttaagcc tctcgactgg ctaccattac ccagagcgat ggcatccacg
    25021 ccatcagctt tgttgccctg gcctatggcg attgcattat ctgccagagc ctggctgcca
    25081 tttcctaatg cgatagtatt gactttatcg gcaaggcttc cgaaccccat tgccatgcta
    25141 ttagtccctt ttgccttcgc attattgcca atagcagttg cattttcagc ggcttcggtc
    25201 tcagcaccta tcgcaatagc gttcgctgcg ctggcaagtg attgtctgcc aaatgcgagg
    25261 ctgtcagtac cgctagctac actgctacgc cccagggcaa ttgcgttttc ttcagatgcg
    25321 acgctacttg ctcccagcgc cattgaatac gcttttgacg cattggcggt gtcgccaaga
    25381 gcaatactca taatctcagt agctttagtg gcatttccca gggcaatggc gtttgctcct
    25441 tccgctttgg aagagtcccc catagcaata gacaatttac ccaatgcttt tgaataacgg
    25501 ccgagggcca ttgagtattg gcccaatgaa tacgaagagg cacccagtgc tatacttcta
    25561 tcgccttctg atattgcact aaccccaaag gccattgatg caccgccaat cgcatgggtt
    25621 tttgacccga tggcagagct atattggcct tcagctatag cgtcataacc cacagcagta
    25681 ctggaaccac tggtgttcat aaaagtattt gctttcgccc ctttgcctat agcaacccag
    25741 ccgtcgccag ctgatccact accgtaatca acaccctgac cgttgtcatt cccggcattc
    25801 gccaatgccc caaacgacga caacattcca cccgcaacca gcgcagaaat taacagctta
    25861 ctgcgcccag atttcttgcc acggcttttt gccgtttcgc tggtaacagt ataattccct
    25921 gtcgcagggt tccagataac tttaaatatt ttgttcatta atgatgctcg ctattccttg
    25981 ttataagcgt actaactgca tttatttttt gcttcttttc tgccgatttt cagcagaggg
    26041 aagattgtcg aactgaatat tttccatgtt gatggctaac ttcagatatc ccaggttatt
    26101 gggcgataca cccttcaggg tcaaattaat gtcttgccat tgacctggta ataatttcaa
    26161 tgtggatttc attgatgtgt tgaaaagtaa ttgttgccac cgggcaaact ctttggcatc
    26221 gacaggtttt tcaccacccc atgtcgcatt aaaaccaata tttgagatag gtgtacttaa
    26281 taagttgacc agtgaaatat gaataacact gccattctct gtggcgtcaa tggataacac
    26341 tcgacccgct aaagccaccg aaccattgtt caggatgaaa tacttcgttt ttgctgggtt
    26401 aaactcggta aaaataaaag tgttttgttg atctttaacg gatttaatta cgtgagttaa
    26461 agtctttgta ctgcttatta tgccagtttg ttgtttttga atagtttcca gttgactctt
    26521 tagctgtgct tgttgttttt caagtagatc aattttattg ttatgttctt cttgttgttt
    26581 caataaacta ctttctggct tgccttcttc aatgtagtca catcccgtta atgcgcagag
    26641 aactgaaaca gtgacagcac tgaaaaaaat cttcttcaag ttcatgtaaa aatccttatt
    26701 tcctccgacc agcgatcaat gccggtagag ttgtccattg tattattggt gatctatgtt
    26761 gctttacatt aatgacagtg atgtgtgtcg attaaatatt gttttaagca ttcatcataa
    26821 aaatgtatca ttcgtttc
//
LOCUS       DAZFQD010000063        26698 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.063, whole genome shotgun sequence.
ACCESSION   DAZFQD010000063 DAZFQD010000000
VERSION     DAZFQD010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26698
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.063"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..134
                     /locus_tag="ACHKTW_002661"
     CDS             <1..134
                     /locus_tag="ACHKTW_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415678.1"
                     /note="interacts RssB preventing the degradation of the
                     stress sigma factor RpoS; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HFO6463659.1"
                     /translation="APFNKDLWSLLKSSQECPPGESKITNKCLHNSCIIKICPYGLK"
     gene            complement(237..1487)
                     /gene="icd"
                     /locus_tag="ACHKTW_002662"
     CDS             complement(237..1487)
                     /gene="icd"
                     /locus_tag="ACHKTW_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HFO6463660.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGDAIIKNM"
     gene            1659..2312
                     /gene="rluE"
                     /locus_tag="ACHKTW_002663"
     CDS             1659..2312
                     /gene="rluE"
                     /locus_tag="ACHKTW_002663"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HFO6463661.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKPENQPTRVILF
                     NKPYDVLPQFTDEAGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWREVTD"
     gene            2322..2783
                     /gene="nudJ"
                     /locus_tag="ACHKTW_002664"
     CDS             2322..2783
                     /gene="nudJ"
                     /locus_tag="ACHKTW_002664"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HFO6463662.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILQASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            2837..3943
                     /gene="mnmA"
                     /locus_tag="ACHKTW_002665"
                     /gene_synonym="trmU"
     CDS             2837..3943
                     /gene="mnmA"
                     /locus_tag="ACHKTW_002665"
                     /gene_synonym="trmU"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFO6463663.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILVVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIEVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            3979..4620
                     /gene="hflD"
                     /locus_tag="ACHKTW_002666"
     CDS             3979..4620
                     /gene="hflD"
                     /locus_tag="ACHKTW_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HFO6463664.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSRQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            4624..5994
                     /gene="purB"
                     /locus_tag="ACHKTW_002667"
     CDS             4624..5994
                     /gene="purB"
                     /locus_tag="ACHKTW_002667"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFO6463665.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQYRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            6162..6833
                     /gene="phoP"
                     /locus_tag="ACHKTW_002668"
     CDS             6162..6833
                     /gene="phoP"
                     /locus_tag="ACHKTW_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HFO6463666.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            6833..8293
                     /gene="phoQ"
                     /locus_tag="ACHKTW_002669"
     CDS             6833..8293
                     /gene="phoQ"
                     /locus_tag="ACHKTW_002669"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HFO6463667.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAKWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            8369..9490
                     /gene="roxA"
                     /locus_tag="ACHKTW_002670"
                     /gene_synonym="ycfD"
     CDS             8369..9490
                     /gene="roxA"
                     /locus_tag="ACHKTW_002670"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HFO6463668.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(9636..10865)
                     /gene="pepT"
                     /locus_tag="ACHKTW_002671"
     CDS             complement(9636..10865)
                     /gene="pepT"
                     /locus_tag="ACHKTW_002671"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFO6463669.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVDRADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHIID
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVTLEGMEK
                     AVQVIVRIAELTAQRKS"
     gene            11115..12251
                     /gene="potA"
                     /locus_tag="ACHKTW_002672"
     CDS             11115..12251
                     /gene="potA"
                     /locus_tag="ACHKTW_002672"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HFO6463670.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFDFVTHDQEEALTMSDRI
                     VVMREGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            12235..13092
                     /gene="potB"
                     /locus_tag="ACHKTW_002673"
     CDS             12235..13092
                     /gene="potB"
                     /locus_tag="ACHKTW_002673"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HFO6463671.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVELE"
     gene            13089..13883
                     /gene="potC"
                     /locus_tag="ACHKTW_002674"
     CDS             13089..13883
                     /gene="potC"
                     /locus_tag="ACHKTW_002674"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HFO6463672.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            13880..14926
                     /gene="potD"
                     /locus_tag="ACHKTW_002675"
     CDS             13880..14926
                     /gene="potD"
                     /locus_tag="ACHKTW_002675"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HFO6463673.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLSNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            14984..15445
                     /locus_tag="ACHKTW_002676"
     CDS             14984..15445
                     /locus_tag="ACHKTW_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001460774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HFO6463674.1"
                     /translation="MSQDSKVLSRAFLGIGLILLVISAIIFYYHFTFTKSAVHTEGII
                     VDAVWYNNHSNDVDDNGSWYPVVAFRPTPDYTLIFNSNIGSDFYEDSEGDRVNVYYPP
                     GHPEQAEINNPWVNFFKWGFVGIAGIIFGSVGLIITLPSTKKSRRKRKSRP"
     gene            15442..16230
                     /gene="ycfZ"
                     /locus_tag="ACHKTW_002677"
     CDS             15442..16230
                     /gene="ycfZ"
                     /locus_tag="ACHKTW_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HFO6463675.1"
                     /translation="MKKFIILLSLLILLPLVATSKPLIPIMKTLFTDVTGTVPDAEEI
                     ARKAELFRQQTGIAPFIVILPDINNEASLRQNGKAMLAHASSSLSNVKGSVLLLFTTR
                     EPRLIMITNGQVESGLDDKHLGLLIENHTLAYLNADLWYQGINNALAVLQAQILKQPT
                     PPLTYYPHPGQQHENAPPGSTNTLGFIAWAATFILFSWIFCYTTRFIYALKFAVAMTI
                     ANMGYQALCLYIDNSFAITRISPLWAGLIGVCTFIAALLLTSKR"
     gene            complement(16369..17190)
                     /gene="cobB"
                     /locus_tag="ACHKTW_002678"
     CDS             complement(16369..17190)
                     /gene="cobB"
                     /locus_tag="ACHKTW_002678"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD+-dependent deacetylase"
                     /protein_id="HFO6463676.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADIFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLKGL"
     gene            complement(17206..18117)
                     /gene="nagK"
                     /locus_tag="ACHKTW_002679"
     CDS             complement(17206..18117)
                     /gene="nagK"
                     /locus_tag="ACHKTW_002679"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045127 - N-acetylglucosamine kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HFO6463677.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGTLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLIFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVCLGNILTIVDPDLVVIGGGLSNFPAITTQLADRLPRHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(18146..19390)
                     /gene="lolE"
                     /locus_tag="ACHKTW_002680"
     CDS             complement(18146..19390)
                     /gene="lolE"
                     /locus_tag="ACHKTW_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HFO6463678.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVDQPWTNWQEALDHVQKVPGIAAAAPYINFTG
                     LVESGVNLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHVAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VIIGVVVSLQLTPIIEWIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(19390..20091)
                     /gene="lolD"
                     /locus_tag="ACHKTW_002681"
     CDS             complement(19390..20091)
                     /gene="lolD"
                     /locus_tag="ACHKTW_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415635.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HFO6463679.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLHNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSRALEMLKAVGLDHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(20084..21283)
                     /gene="lolC"
                     /locus_tag="ACHKTW_002682"
     CDS             complement(20084..21283)
                     /gene="lolC"
                     /locus_tag="ACHKTW_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HFO6463680.1"
                     /translation="MYQPVALFIGLRYMRGRAADRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRMMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGNEMLVNIEDASRLMRYPAGN
                     ITGWRLWLDEPLKVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            21691..25137
                     /gene="mfd"
                     /locus_tag="ACHKTW_002683"
     CDS             21691..25137
                     /gene="mfd"
                     /locus_tag="ACHKTW_002683"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFO6463681.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGVLIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLETSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDKVRNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDVARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            25281..26243
                     /gene="ldtC"
                     /locus_tag="ACHKTW_002684"
     CDS             25281..26243
                     /gene="ldtC"
                     /locus_tag="ACHKTW_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
                     /protein_id="HFO6463682.1"
                     /translation="MMIKTRFSRWLTFFTFAAAVALALPAKANTWPLPPAGSRLVGEN
                     KFHVVENDGGSLEAIAKKYNVGFLALLQANPGVDPYVPRAGSVLTIPLQTLLPDAPRE
                     GIVINIAELRLYYYPPGKNSVTVYPIGIGQLGGDTLTPTMVTTVSDKRANPTWTPTAN
                     IRARYKAQGIELPAVVPAGPDNPMGHHAIRLAAYGGVYLLHGTNADFGIGMRVSSGCI
                     RLRDDDIKTLFSQVTPGTKVNIINTPIKVSAEPNGARLVEVHQPLSEKIDDDPQLLPI
                     TLNSAMQSFKDAAQTDAEVMQHVMDVRSGMPVDVRRHQVSPQTL"
     gene            complement(26326..26583)
                     /gene="bhsA"
                     /locus_tag="ACHKTW_002685"
                     /gene_synonym="ycfR"
     CDS             complement(26326..26583)
                     /gene="bhsA"
                     /locus_tag="ACHKTW_002685"
                     /gene_synonym="ycfR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple stress resistance protein BhsA"
                     /protein_id="HFO6463683.1"
                     /translation="MKNVKTLIAAAILSSMSFASFAAVEVQSTPEGQQKVGTISANAG
                     TNLGSLEEQLAQKADEMGAKSFRITSVTGPNTLHGTAVIYK"
BASE COUNT         6609 a         6902 c         6740 g         6447 t
ORIGIN      
        1 tcgcaccatt caacaaggat ttatggagtc tactcaaaag cagtcaagag tgtcctccag
       61 gagaaagcaa aataacaaat aaatgtttgc ataatagttg cattataaaa atatgcccat
      121 atgggctcaa gtaaagggtt ttatataaat atgctcgata tgctattaat aatacctaca
      181 taaataaaac aacgggcgtg ttatacgccc gttgcaatat taaacacatg tagtgattac
      241 atgttcttga tgatcgcgtc accaaattct gaacatttca gcagtttagc gccttccatc
      301 agacgctcga agtcataggt tacggtcttc gcattgattg cgccttccat acctttaaca
      361 atcaggtctg cggcttcagt ccagcccata tggcgtaaca tcatctcagc ggagagaata
      421 atagagccag ggtttacttt gtcctgaccg gcgtatttcg gcgcagtacc gtgggtggct
      481 tcaaacaggg cgcattcgtc accgatgttt gctccagggg cgataccgat accgccaacc
      541 tgcgctgcca gagcgtcaga aatgtagtca ccgttcaggt tcatacaggc gataacatca
      601 tattcagccg gacgcagcag gatctgttgc aggaatgcat cagcaatcac gtctttaatg
      661 acgatctctt tgccggtgtt cgggttttta actttcagcc acgggccgcc gtcgatcagt
      721 tcaccgccaa actcttcacg cgccagctgg tagccccagt ctttaaacgc gccttcggtg
      781 aacttcatga tgttgccttt gtgcaccaga gtcacagagt cacgatcgtt agcaattgcg
      841 tattcgatcg ctgcacgaac cagacgtttg gtgccttctt ccgaacacgg cttaataccg
      901 ataccacaat gttccgggaa gcgaattttc ttcaccccca tctcttcacg caggaattta
      961 atcactttct cggcgtcggc agagtctgct ttccattcga tacccgcata aatgtcttcc
     1021 gagttttcac ggaagataac catatcggtc agttcagggt gtttaaccgg gcttggagtg
     1081 ccctgatagt aacgtaccgg acgcaggcag atgtagagat ccagttcctg gcgcagggca
     1141 acgttcagag agcgaatacc gccaccaaca ggagtggtca gcggaccttt aatggcaacg
     1201 cgatattcac gaatcagatc aagggtttca gcaggcagcc agacatcctg accataaacc
     1261 tgtgtggatt tttcaccggt gtaaatttcc atccaggaga ttttacgctc gcctttatag
     1321 gctttctcga ctgcagcgtc gaccactttc agcatggctg gggttacatc tacaccgatt
     1381 ccatcacctt caatgtaagg gataatcgga ttttcaggaa cgttgagttt gccgttttgc
     1441 agggtgatct tcttgccttg tgccggaaca actactttac tttccattca cctctccttc
     1501 gagcgctact ggtttgctcg tctgccacca cgttgcatat gcgtttgcgt cctgcgatac
     1561 ggatgcttta gagcaatttt ttgttaatga tttgtaattg gcttgtcaat actaccctat
     1621 tgtttgccgt catgaaagat gcgcgttatt aggctataat gcggcaattc ataatctctg
     1681 aaaataccat gcaaaaaact tcttttagaa atcaccaggt taagcgattc agctcgcaac
     1741 gttctaccag gcgtaaacct gaaaaccagc ccacgcgtgt gatcctgttc aataaaccct
     1801 acgatgttct tccgcagttc accgatgaag ccggacgcaa aacattaaaa gaattcatcc
     1861 cggttcaggg cgtttatgca gcaggtcgtc ttgaccgcga tagcgaaggg ttactggtgc
     1921 tgaccaataa cggcgcgttg caggcgcgtt taacccagcc gggcaaacgc accgggaaaa
     1981 tctattatgt gcaggtggaa ggtattccca cacaagacgc acttgaagcc ttgcgcaatg
     2041 gcgtaacctt aaatgatggc cctaccctgc ccgcaggcgc ggagctggtt gacgaacccg
     2101 cgtggttatg gccgcggaat ccaccaattc gtgaacgcaa aagtattccc accagctggc
     2161 tgaagatcac cttatatgaa ggacgtaatc gccaggtgcg ccgcatgacc gcccatgttg
     2221 gtttccccac gctgcgactg attcgctatg cgatgggaga ttactctttg gataatcttg
     2281 ccaatggcga atggcgagaa gtgacagatt aaggatgcaa aatgtttaaa ccgcacgtta
     2341 ccgttgcttg cgtggtgcac gcagaaggca aatttttagt cgttgaagag acgattaatg
     2401 gtaaagcgtt atggaaccaa cctgccgggc atctggaagc cgatgaaacc ttagtggaag
     2461 ccgccgcccg tgaactgtgg gaagaaaccg gcatcagcgc gcagccgcaa cactttattc
     2521 gtatgcatca gtggattgcg ccagataaaa cgccgttttt gcgtttcctc tttgccattg
     2581 agcttgagca aatatgcccg acccagcctc atgacagcga tatcgactgc tgtcgttggg
     2641 tcagcgccga agaaatttta caggcgtcaa atcttcgttc gccgctggtg gcggaaagta
     2701 tccgttgtta tcaaagcggg caacgctatc cgctggagat gattggcgat tttaactggc
     2761 cttttacaaa gggtgtcatc taaaaggctg cgtgatagaa tacgccgcct tgaagttcaa
     2821 tgtcgtgagt gatccaatgt ctgaaaccgc aaaaaaagta atcgtcggca tgtccggcgg
     2881 tgtcgattcc tccgtttctg cctggctgtt gcaacaacag ggatatcagg ttgaaggcct
     2941 gtttatgaag aactgggaag aagacgacgg tgaggaatat tgcacagcgg cagcggatct
     3001 ggctgatgcc caggctgtct gcgacaagct cggcattgaa ctgcacaccg ttaactttgc
     3061 tgccgagtac tgggacaacg tcttcgaact gttccttgcc gaatataaag ccggtcgcac
     3121 gccgaatccg gatattctgt gcaacaaaga gatcaaattt aaagccttcc tcgaatttgc
     3181 cgccgaagat ttaggtgccg attatatcgc taccggtcat tacgtacgtc gggccgatgt
     3241 cgatggcaag agccgcctgc tgcgtggtct ggacagcaat aaagaccaga gctacttcct
     3301 ttatacgctc agccatgagc agattgcgca aagcctgttc ccggtcggcg aactggaaaa
     3361 accgcaggtg cgtaagattg ctgaagatct tggtctggtc accgcgaaga aaaaagactc
     3421 taccggcatt tgcttcattg gcgaacgtaa attccgcgag ttcctgggcc gttatctccc
     3481 ggcgcaaccg ggcaaaatca ttaccgtcga tggcgatgaa attggtgagc accaggggct
     3541 gatgtatcac actctcggtc agcgtaaagg tctgggtatc ggtggcacca aagaaggtac
     3601 cgaagaaccg tggtatgtgg tggacaaaga cgtcgaaaac aacattctgg ttgtcgctca
     3661 gggccatgaa cacccgcggc tgatgtctgt cgggttgatt gcccagcagt tgcactgggt
     3721 cgatcgcgaa ccattcaccg gcactatgcg ttgcacggta aaaacccgct atcgccagac
     3781 cgatatccct tgcaccgtca aggcgctgga cgatgatcgc attgaagtga ttttcgatga
     3841 accggttgcc gccgtgacgc cgggccagtc tgccgtcttc tataacggtg aagtgtgcct
     3901 cggtggcggt attattgagc agcgtctgcc gctgccggtc tgattattat ctttacttaa
     3961 aacaaggaag cagtgaacgt ggcaaagaat tactatgaca tcaccctcgc cctggccggt
     4021 atttgtcagt cggcacgcct ggtgcaacaa ctcgctcacc aggggcattg tgatgccgat
     4081 gcgctacacg tctcactcaa cagtattatt gatatgaacc ccagctcgac gctggcggtt
     4141 tttggcggta gcgaagccaa cctgcgcgtc gggctggaaa ccctgctcgg cgtgctcaat
     4201 gccagcagtc gccagggctt aaacgctgag ttaacccgct acacactcag cttgatggtg
     4261 cttgagcgca aactctcctc agcgaaaggc gcgctcgaca ctctgggcaa ccggatcaac
     4321 ggcctgcaac gccagctcga acacttcgat ttacagtccg aaacgctgat gagcgcgatg
     4381 gctgctatct atgttgatgt gatcagcccg cttggcccgc gcattcaggt caccggttcc
     4441 cctgctgtac tgcaaagccc acaagtgcag gcgaaagttc gcgcaaccct gctggcaggc
     4501 attcgcgccg ccgtgctctg gcaccaggtc ggcggcggac gtctgcaact gatgttttct
     4561 cgtaatcgcc tgaccactca ggcaaaacaa attcttgctc atttaacccc ggagttgtga
     4621 tctatggaat tatcctcact gaccgccgtt tcccctgtcg atggacgcta cggcgataaa
     4681 gtcagcgcgc tgcgcgggat tttcagcgaa tatggtttgc tgaaattccg tgtacaagtt
     4741 gaagtacgtt ggctgcaaaa actggccgcg cacgcagcga tcaaggaagt tcctgctttt
     4801 gctgccgacg caatcggtta ccttgatgca attgtcgcca atttcagcga agaagatgcc
     4861 gcacgcatca aaaccatcga gcgtaccact aaccacgacg ttaaagcggt tgagtatttc
     4921 ctgaaagaaa aagtggcgga gatcccggaa ctgcacgcgg tttctgaatt catccacttt
     4981 gcctgtactt cggaagatat caataacctc tcccacgcat taatgctgaa aaccgcgcgt
     5041 gatgaagtga tcctgccgta ctggcgtcaa ctgattgatg gcattaaaga tctcgccgtt
     5101 cagtaccgcg atatcccgct gctgtctcgt acccacggtc agccagccac gccgtcaacc
     5161 atcggtaaag agatggcaaa cgtcgcctac cgtatggagc gccagtaccg ccagcttaac
     5221 caggtggaga tcctcggcaa aatcaacggc gcggtcggta actataacgc ccacatcgcc
     5281 gcttacccgg aagttgactg gcatcagttc agcgaagagt tcgtcacctc gctgggtatt
     5341 caatggaacc cgtacactac ccagattgaa ccgcacgact acattgccga actgtttgat
     5401 tgcgttgcgc gcttcaacac cattctgatc gactttgacc gtgacgtctg gggttatatc
     5461 gcccttaacc acttcaaaca gaaaaccatt gctggtgaga ttggttcttc caccatgccg
     5521 cataaagtta acccgatcga cttcgaaaac tccgaaggga atctgggcct ttccaacgcg
     5581 gtattgcagc atctggcaag caaactgccg gtttcccgct ggcagcgtga cctgaccgac
     5641 tccaccgtgc tgcgtaacct cggcgtgggt atcggttatg cgttgattgc gtatcaatcc
     5701 accctgaaag gcgtgagcaa actggaagtg aaccgtgacc atctgctgga tgagctggat
     5761 cacaactggg aagtgctggc agaaccaatt cagacagtta tgcgtcgcta tggcatcgaa
     5821 aaaccgtacg agaagctgaa agagctgact cgtggtaagc gcgttgacgc cgaaggcatg
     5881 aagcagttta tcgacggtct ggcgttgcca gaagaagaga aagcccgcct gaaagcgatg
     5941 acgccggcaa actacattgg tcgcgccatc accatggttg atgagctgaa ataaactcgt
     6001 atcagtgccg gatggcgatg ctgtccggcc tgcttattaa gattatccgc tttttatttt
     6061 ttcactttac ctcccctccc cgctggttta tttaatgttt acccccataa ccacataatc
     6121 gcgttacact attttaataa ttaagacagg gagaaataaa aatgcgcgta ctggttgttg
     6181 aagacaatgc gttgttacgt caccacctta aagttcagat tcaggatgct ggtcatcagg
     6241 tcgatgatgc agaagatgcc aaagaagccg attattatct caatgaacat ttaccggata
     6301 ttgcgattgt cgatctcgga ttgccagacg aggacggtct gtcactgatt cgccgctggc
     6361 gtagcaatga tgtttcactg ccgattctgg tattaaccgc ccgtgaaagc tggcaggaca
     6421 aagtcgaagt attaagtgct ggtgctgatg attacgtgac taaaccattt catattgaag
     6481 aggtgatggc gcgaatgcag gcattaatgc ggcgtaatag cggtctggct tcacaggtca
     6541 tttcgctacc accgtttcag gttgatctct ctcgccgtga attatctatt aatgacgaag
     6601 tgatcaaact gaccgcgttc gaatacacca ttatggaaac gttgatacgc aataatggca
     6661 aagtggtgag caaagattcg ttaatgctcc aactctatcc ggatgcggag ctgcgggaaa
     6721 gccataccat tgatgtactg atgggacgtc tgcgcaaaaa aattcaggca caatatccgc
     6781 aagaagtcat taccaccgtt cgcggccagg gctacctgtt cgaattgcgc tgatgaaaaa
     6841 attactgcgt ctttttttcc cgctctcgct gcgggtacgt tttctgttgg caacggcagc
     6901 ggtagtactg gtgctttcgc ttgcctacgg aatggtcgcg ctgatcggtt atagcgtcag
     6961 tttcgataaa actacgtttc ggctgttacg tggcgagagc aatctgttct atacccttgc
     7021 gaagtgggaa aacaataagt tgcatgtcga gttacccgaa aatatcgaca agcaaagccc
     7081 caccatgacg ctaatttatg atgagaacgg gcagctttta tgggcgcaac gtgacgtgcc
     7141 ctggctgatg aagatgatcc agcctgactg gctgaaatcg aatggttttc atgaaattga
     7201 agcggatgtt aacgatacca gcctcttgct gagtggagat cattcgatac agcaacagtt
     7261 gcaggaagtg cgggaagatg atgacgacgc ggagatgacc cactcggtgg cggtaaacgt
     7321 ctacccggca acatcgcgga tgccaaaatt aaccattgtg gtggtggata ccattccggt
     7381 ggagctaaaa agttcctata tggtctggag ctggtttatc tatgtgctct cagccaatct
     7441 gctgttagtg atcccgctgc tgtgggtcgc cgcctggtgg agtttacgcc ccatcgaagc
     7501 cctggcaaaa gaagtccgcg aactggaaga acataaccgc gaattgctca atccagccac
     7561 aacgcgagaa ctgaccagtc tggtacgaaa cctgaaccga ttgttaaaaa gtgaacgcga
     7621 acgttacgac aaataccgta cgacgctcac cgacctgacc catagtctga aaacgccact
     7681 ggcggtgctg caaagtacgc tgcgttctct gcgtagtgaa aagatgagcg tcagtgatgc
     7741 tgagccggta atgctggagc aaatcagccg catttcacag caaattggct actacctgca
     7801 tcgtgccagt atgcgcggcg ggacattgct cagccgcgag ctgcatccgg tcgccccact
     7861 gctggacaat ctcacctcag cgctgaacaa agtgtatcaa cgcaaagggg tcaatatctc
     7921 tctcgatatt tcgccagaga tcagctttgt cggtgagcag aacgattttg tcgaggtgat
     7981 gggcaacgtg ctggataatg cctgtaaata ttgcctcgag tttgtcgaaa tttctgcaag
     8041 gcaaaccgac gagcatctct atattgtggt cgaggatgat ggccccggta ttccattaag
     8101 caagcgagag gtcattttcg accgtggtca acgggttgat actttacgcc ctgggcaagg
     8161 tgtagggctg gcggtagccc gcgaaatcac cgagcaatat gagggtaaaa tcgtcgccgg
     8221 agagagcatg ctgggcggtg cgcggatgga ggtgattttt ggtcgccagc attctgcgcc
     8281 gaaagatgaa taaatatgtc catacttcac gcattacgtt aagcatccgt tataatcggt
     8341 tgcagatacc agcctgtgga tgcttaacat ggaataccaa ctcactctta actggcccga
     8401 ttttcttgaa cgtcactggc agaaacgccc ggtggtgtta aaacgcggct ttaataattt
     8461 tattgacccg atctctccag acgagttggc gggtctggcg atggaaagcg aagttgacag
     8521 tcgactggtc agtcaccagg atggcaaatg gcaggtcagc cacggcccgt tcgaaagcta
     8581 cgatcatctc ggtgaaacca actggtcatt actggtacag gcagtaaacc actggcatga
     8641 gccgaccgcc gcgctgatgc gaccgttccg tgaactaccg gactggcgta ttgatgatct
     8701 gatgatctct ttttctgtac ctggcggcgg cgtcggcccg catctcgatc agtacgacgt
     8761 gtttatcatt cagggtaccg gacgtcgtcg ctggcgagtg ggcgaaaagc tgcaaatgaa
     8821 acagcactgc ccacatccgg atctgttaca ggtcgatccg ttcgaagcca tcatcgatga
     8881 agagctggag cctggtgata ttctttatat tccgccagga ttcccgcatg aaggctacgc
     8941 gctggaaaat gcgatgaact attccgtggg ctttcgcgcg ccaaatacgc gggaactgat
     9001 tagtggattt gccgattatg tgctgcaacg tgaactgggc ggcaactact acagcgatcc
     9061 tgatgttcca cctcgcgctc atcctgcgga tgttctgccg caagagatgg ataaactgcg
     9121 tgagatgatg ctcgaattga tcaaccagcc ggaacacttt aagcaatggt ttggcgagtt
     9181 tatatcccag tcacgtcatg aactggatat cgcgccgccg gaaccgcctt atcagccaga
     9241 tgaaatctac gatgcgctga aacaaggtga tgtgctggtg cgcctgggtg gtctgcgcgt
     9301 attgcgcatt ggcgacgacg tgtatgccaa tggtgagaag atcgattccc cgcaccgtcc
     9361 ggcactggat gcactcgcca gcaacattgc gctgactgcg gagaattttg gcgatgcgct
     9421 ggaagatccg tcattcctcg cgatgctcgc ggcgctggtc aatagcgggt attggttctt
     9481 cgaagggtaa gtttggattt aagccggatg cggcgctgat tgcgccttat cgtgcctaca
     9541 tgcactgaac gcctgtaggc acgataagta tgttgaccag tatcaatata acggtttcat
     9601 cccggtaatt ggcagtccac atacgcatcg ggcaatcacg acttccgttg cgccgttaac
     9661 tcggcaatac ggacaatcac ctgcaccgct ttttccatac cttccagagt cacaaactca
     9721 tgcttaccat gatagttgta accgccagtg aacaggttcg ggcacggtaa tcccataaac
     9781 gacaactgcg cgccgtcggt accgccgcgg atcggtttca gttccggttc aatatcgcag
     9841 tcacgcatcg cctgctgggc gatatcgata atatgcggat gctcaaccac tttctcgcgc
     9901 atattgtagt aactgtcttc aatcaccagt tcaatgtagc aatcaggatg taaccctttg
     9961 cccacttttt tggcgatctc catcatttta cgtttacgcg cttcaaactg tttacggtcg
    10021 aaatcacgga tgatgtagtg catatcggct cggtcaacgg tgcctttcat gctcgccagg
    10081 tgatagaaac cttcatagcc ttctgtcatt tccgggcttt catccgccgg aacttccgca
    10141 tgaatacgtg ccgccagcga cagcgcattt accatcactc ctttcgccgt gcccggatga
    10201 acattgttac cgacaatttt gatattgacc gatgcggcgt tgaagttttc aaactccagt
    10261 tcacctacgc caccaccgtc aacagtgtaa gcccagcggg catcgaaggc atcaacatca
    10321 aaatgtttcg cccctttgcc cacttcttca tccggggtaa aggcgacgcg aatatcacca
    10381 tgcggaatgt ttttctgttg caataccgcc agcgcggtca tgatttctgc aataccggct
    10441 ttgtcatcgg cacctaacaa ggttttacca tcggtagtaa tcagcgtctg acccagtagc
    10501 tgatgcagca ccgggaacat aaccggtgat aaaacttcat cgccgatacc cagcgcaata
    10561 tcgccaccgc gatagttttc aacaatttgc ggattcacat ttttgccgct gcaatccggt
    10621 gaggtatcca catgagaaat aaagccaatc gccgggatat cgccagggac gttagccggt
    10681 aacgtcgcca tcaaagtgcc cttctcactt aaggtcacat tgataagccc catctcttcg
    10741 agctgctctt tcagcagatg caataacttc cattggcctt ccgtgctggg aacctgtctc
    10801 acccctgctt ttgattgggt atccagagac acgtagttca aaaatcgctc aagtagttta
    10861 tccatgtagt caccctcact ttttgtgaca acattattaa gaagcaagaa aagacaaata
    10921 ttgcgtcagg tcacttttac ccctgcaagc gggaatattt atcagcatta ccttaatgaa
    10981 tataaagcta agccagtaat tcacaacaaa gattggcgat tcaagcggtt tgccgtagaa
    11041 ttaccgccct cattaagagt caccaaggtg gttaaccaca aaccccgcat cggtaagcca
    11101 tccgttgcgt ttacatggga cagagtaaaa aattgaataa acaaccgagt tcgctttcac
    11161 cgctggtgca attggcggga attcgcaaat gctttgatgg taaagaggtc attccccagc
    11221 tggatctgac tatcaacaat ggcgagttcc tcacgctgct tggcccttct ggctgcggta
    11281 aaacaaccgt tcttcgcctg attgcaggtc tggaaactgt tgattccgga cgcatcatgc
    11341 tggataacga ggacatcacc cacgttccgg cggaaaaccg ctatgtgaac actgttttcc
    11401 aaagctacgc acttttcccc cacatgaccg tgttcgaaaa tgtggccttt gggttgcgca
    11461 tgcaaaaaac ccccgctgct gaaattacgc cccgcgtgat ggaagccctg cggatggtgc
    11521 aactggaaac cttcgctcaa cgtaaaccgc atcagctctc tggtggtcaa cagcaacgcg
    11581 tcgccattgc tcgcgcggtg gttaacaagc ctcgtctgtt gttgctggat gagtcgctct
    11641 cagcgctgga ttacaaactg cgtaagcaaa tgcagaacga gctgaaagcg ttacagcgta
    11701 agcttggtat taccttcgac tttgtcactc acgatcagga agaagcgctc accatgtcag
    11761 acaggattgt ggtgatgcgc gagggtcgta ttgagcaaga tggtacaccg cgcgaaattt
    11821 acgaagagcc gaaaaacctg tttgttgccg gcttcattgg cgaaatcaat atgtttaacg
    11881 ccactgtcat cgaacgtcta gacgagcagc gcgtacgcgc caacgttgaa ggccgcgaat
    11941 gtaatatcta cgttaacttc gccgttgaac cggggcaaaa actgcatgtt ctgctgcgcc
    12001 cggaagactt acgtgttgaa gagattaacg acgacaacca cgctgaaggg ctgattggtt
    12061 acgttcgtga gcgtaactac aaaggcatga cgctggagtc ggttgttgaa ctggaaaatg
    12121 gcaagatggt gatggtcagc gaattcttca atgaagacga ccctgacttt gaccactctc
    12181 tcgaccaaaa aatggccatt aattgggtag aaagctggga ggtcgtactg gctgatgaag
    12241 aacacaagta agttccagaa tgtagtgatt gtcactattg tcggttggct tgtgttgttt
    12301 gtctttctgc ccaacctgat gatcattggc accagctttt tgacccgcga cgacgccagt
    12361 ttcgtcaaaa tggtctttac gctggataac tacacgcgtc tgctcgatcc gctctatttt
    12421 gaagtgctat tgcactcgct gaatatggcg ctgatcgcca ccctcgcctg cctggtgctg
    12481 ggctacccgt ttgcctggtt tctagcgaag ctaccacaca aggtgcgtcc gctgctgctg
    12541 tttctgctga ttgttccctt ctggaccaac tcactgattc gcatctacgg gctgaaaatt
    12601 ttcctcagca ccaaaggcta tctcaacgag tttttgctct ggctgggcgt tatcgacaca
    12661 ccaattcgta tcatgttcac gcccagtgcg gtgattatcg gtctggttta cattctgcta
    12721 ccgtttatgg tgatgccgct gtactccagt atcgaaaaac tggataagcc gttgcttgag
    12781 gcggcgcgcg atctcggtgc cagcaagtta cagactttta tccgtatcat tattccactg
    12841 acgatgccgg gaattattgc cggatgtctg ctggtgatgc tgccagcgat gggcctgttc
    12901 tatgtatccg acctgatggg cggtgcgaaa aacctgctga tcggtaacgt catcaaggtc
    12961 cagttcctca atattcgtga ctggccgttt ggtgcagcca ccagcattac gctgactatc
    13021 gtaatgggcc tgatgttgct ggtttactgg cgcgcttctc gtctgctgaa taaaaaggtg
    13081 gaactcgaat gatcggtcga ctgcttcgcg gcggttttat gaccgctatc tacgcgtacc
    13141 tgtatatccc aatcattatt ttgattgtga actcctttaa cagctcgcgc tttggcatca
    13201 actggcaggg ttttaccacc aaatggtata gcctgctgat gaacaacgac agcctgttac
    13261 aggcagcgca gcattcacta acaatggcgg tgttttcggc gacatttgct acgcttatcg
    13321 gttcactgac ggcagttgcg ctgtaccgtt atcgctttcg tggtaagccg ttcgttagcg
    13381 gaatgctgtt tgtggtgatg atgtcaccag atatcgtgat ggcgatttct ctgctggtgc
    13441 tgtttatgct gctgggtatt cagcttggct tctggtcgct gctgttctcg catatcacct
    13501 tctgcctgcc gtttgtggtg gtgacggtgt attcgcgcct gaaaggtttt gacgtgcgga
    13561 tgctggaagc ggcgaaagat ctcggtgcca gtgaatttac cattctgcga aaaatcattc
    13621 tgccactggc aatgcctgcg gtggcggcgg gctgggtgtt aagctttacc ctgtcgatgg
    13681 acgatgtggt agtttcttcg ttcgtcaccg gaccaagtta tgaaattctg ccgttaaaaa
    13741 tttattcgat ggtcaaagtc ggcgtatcgc cggaagttaa cgcgctggca accatattac
    13801 tggtgctgtc gctggtgatg gttattgcca gccagcttat tgctcgtgat aaaacgaaag
    13861 gtaacacagg ggacgttaaa tgaaaaaatg gtcacgccac ctgctcgcgg cgggtgctct
    13921 ggcactgggc atgagcgccg ctcacgccga tgacaacaac acgctgtatt tctacaactg
    13981 gaccgagtac gtgccgccag gactgcttga acagttcacc aaagaaaccg gtattaaggt
    14041 tatctattcg acttacgagt cgaacgaaac catgtacgcc aagctgaaaa cgtacaaaga
    14101 cggtgcctat gacctggtgg ttccttcaac ctattacgtc gataaaatgc gtaaagaagg
    14161 gatgatccag aagatcgaca agtcgaagtt aagcaatttc agcaacctcg atccagacat
    14221 gctcaacaag ccgtttgacc cgaataacga ctactccatt ccgtatatct ggggggcgac
    14281 ggcgattggc gttaacggtg atgcggtaga tccgaaatcc gttaccagct gggccgatct
    14341 gtggaagcct gagtacaaag gtagcctgct gttgaccgac gatgcccgtg aagtgttcca
    14401 gatggcgctg cgtaagctgg gctactccgg taacaccacc gatccgaaag agattgaagc
    14461 tgcatataac gagctgaaaa aactgatgcc aaacgtcgca gcgtttaact ccgataaccc
    14521 ggctaacccg tacatggaag gcgaagttaa cctcggcatg atctggaacg gttctgcttt
    14581 cgttgcacgc caggcgggta cgcctattga cgtggtgtgg ccgaaagaag gcggcatttt
    14641 ctggatggac agcctggcga tcccggcaaa tgccaaaaac aaagaaggcg cgctgaaatt
    14701 gatcaacttc ctgctgcgcc cggatgtggc aaaacaggtt gccgaaacta tcggttatcc
    14761 aacgccaaac cttgcggcgc gtaagctgtt aagcccggaa gtggcgaacg ataaaacgct
    14821 ctacccggat gctgaaacca ttaaaaatgg tgaatggcag aatgacgttg gcgcagccag
    14881 cagcatttat gaagagtatt atcagaagct gaaagcagga cgttaattca gcaagcgggc
    14941 ggtggcatta ccgcccgttt tttatttagc aaggatgtgg ctaatgagtc aggacagtaa
    15001 agtgttatcc cgcgcttttc tcggtatcgg tctgatattg ctcgttattt ctgcaatcat
    15061 cttttattac cattttactt ttaccaaaag cgcggttcat acagaaggaa tcattgttga
    15121 tgccgtttgg tacaacaatc actcaaacga cgtcgatgat aatggttcct ggtatccagt
    15181 ggttgccttt cgccctacgc cagattacac gctaattttc aattcgaata tcggtagtga
    15241 tttttatgaa gatagcgaag gtgacagagt caatgtctat tatccgccag ggcatccaga
    15301 acaagccgaa ataaacaatc cctgggttaa tttttttaaa tggggctttg ttggcatagc
    15361 aggcattatt ttcggtagtg tcggtctgat aatcacgctc ccctccacca aaaaatctcg
    15421 caggaaacgg aagtcccgcc catgaaaaaa ttcattatac tgctcagttt actgatactc
    15481 ctgcctctgg tagcaaccag taaaccattg attccaataa tgaagacact ttttacggat
    15541 gttaccggta ctgtaccgga tgctgaagag atcgcacgta aagccgagct ttttcgccaa
    15601 caaacgggaa ttgcaccgtt tattgtgatt ttacccgaca ttaataatga agccagtctc
    15661 agacaaaatg gtaaagcgat gctggcgcat gcgtcatctt cattgagtaa tgtaaaagga
    15721 agtgttctgt tactatttac tacccgcgaa ccacggttaa ttatgatcac caacggccag
    15781 gttgaaagtg gtctggacga taaacatctc ggccttctga tagaaaatca cacgctggct
    15841 tatttaaatg cagatctctg gtatcaggga atcaataatg cattggctgt tctacaagca
    15901 cagatattaa aacaaccgac gccgccactg acgtattatc cgcatccagg acaacagcat
    15961 gaaaatgctc cccccggcag caccaacaca ttaggtttta tcgcctgggc tgcgacgttt
    16021 atcttatttt cctggatctt ttgctacacg actcgtttta tttatgcgtt aaaatttgca
    16081 gtggcgatga ctattgccaa tatgggttat caggctttat gtttgtatat cgataatagc
    16141 tttgcgatta ccagaatatc gccactctgg gcagggctaa ttggtgtctg tacgtttatt
    16201 gccgcattgt tattgacgag taaacgataa aagaggccgg aaaagtgcgc gtgtattatg
    16261 ccggatgggg cgcgaacgcc ttatccggcc cacaaaaccc gcaaattcaa ttaattgcgt
    16321 ccccttgtag gcctgataag cgtagcgcat caggcaatgc ttcctgcttt ataatccctt
    16381 cagcaacttt tcaacaaact caggcaccac ctggcttgcc gggccgtaat atttctcggc
    16441 aaattcatta ccgacctgac ttggttcgag attcagttcc acggtgtgcg cgccatgcag
    16501 tttcgcttcg tgaacaaacc cagccgccgg ataaacatgc ccggaagtac caatggcaat
    16561 gaaaatatca gccatcgaca acgccatata aatttcatcc atgccgagtg gcatttcgcc
    16621 aaaccaaacg acgtgcgggc gcaagggtgc cgggaactgg caacaatggc atttatcttc
    16681 tggcgtaacg tctccggtcc agtcgagaac ctgaccactt tgcgaacaac gaactttcag
    16741 caattcccca tgcatatgaa tgacattggt attacctgcg cgttcatgca ggttgtctat
    16801 attctgcgtt accagcaaaa agcgatcgcc gagggcgtct tgcagtttag ccagcgcaag
    16861 atgcgcggcg ttaggctgaa tttctggctg ctgcagctgt cgacgacggg cgttataaaa
    16921 cgcttgcacc agttcaggat cgcgatcgaa accttccggc gttgccacat cttcaacccg
    16981 atgttcttcc cacaggccat ctgcggcgcg aaaggtacga atacctgatt ccgcagaaat
    17041 ccctgcccct gtcagtacga gtactcttgg tttttccatt gcttccggca ccactttatc
    17101 tctgaaaaaa atacgctgac gcaaacgctc gcgcaggcgg cgtttatttt tacgaaaacg
    17161 acttaaccga tgaccccgac gcgacagcat agcaacctct gttgtttaat cggttagatg
    17221 taggaaggcc gcaccacgca ttcctcccgc atcaccgtgg cgcgcgcgtt caatgcgcgg
    17281 aacacgagct acaggtaaga gatgacgagg cagcctgtcc gccagttgcg ttgtgattgc
    17341 cgggaaattc gataagccac caccaatgac gaccaggtca gggtcaacaa tggtcaggat
    17401 atttcccaga caaaccgcta ataaatccag ataacgctca acgtgcgccc ttgcctgctc
    17461 atcgccttga tcataaagcg caataatttc gggagcctgc aacggttgat gataatagtg
    17521 ttgatacagc cacgcaaaac cgcgaccaga cagataattt tcaatgcagc catgctgacc
    17581 acagccgcag cggcgtaacg ggaaatccaa ccccatcatg gttaacgcat caaccggcag
    17641 acgcatatgg ccaaactcgc cggtaatgta gcttttaccg gtaatcggtt tgccgttgaa
    17701 aatcagcccg ccgccaacgc cggtgccgag aatcaacccc atcaccagtg gatattgagt
    17761 aaattcatca tcccaggctt ctgaaagggc aaaacagttg gcatcgttat caaggcgtac
    17821 atcgcgatca agacgtgcgc tcaggtcggc acgcagcggt ttaccgctgg cagcggggac
    17881 attggcggca tacagcgtac catcttctgt ttccggcatt cccggaatac cgatgccgac
    17941 agaaccttta cagccaaaac gttggtcagc ttcagctacc agctcacaca ctgcatctaa
    18001 aaatgcgtca tagctgtcac gcggtgtcgg cacccgcttt tcccactgca actgccgacc
    18061 gctatcaaac acgccaagcg caatttttgt tccaccaata tcaaacccgt aatacattgc
    18121 cgctccttgt tttaatgtac tgcctttact ggccgctaag gactcgcgca gggtcaatat
    18181 tactggcgcg ccgcgccgga taccaacttg ccaaaagact cagcaacaat gctgtgacca
    18241 gtacgtagaa gacgtccagc cagtgcaatt ccgatggcag gaaatcaata aaatagatat
    18301 cgctggagag gaactgatga ccgatcaact tttcaatcca ctcaataatc ggggtaagtt
    18361 gcagtgaaac caccacaccg ataatcaccc cacacaggct gccgaacagc cctgccagca
    18421 atccatacca gacaaagatg gcgcgaatta aaccatcttt cgcccccagc gttcttaata
    18481 ctgcgatatc gccactcttg tctttcaccg ccatcactaa ggtggagacg atgttgaaac
    18541 aggccacgcc aatcaccagt accatcgcca gatacataat ggcgcggatc atctggatat
    18601 cgcgatacat atagccgtaa gtaccaatcc aacttttaat ataaacatag ctgttggtca
    18661 cttcacccgc atcgcgtacc agcttattgg cgttgaaaac atccgtcatt ttaagggcaa
    18721 tacctgacac gctggaaccc atatcaagat attgttgggc atccgccagc gggatcatgg
    18781 caaaactgtg atcgagttga ccactcaact gcaaaatacc ggcaacgtgc aaacgcacac
    18841 gttttggctg catcagttta tgctcgggat tcgagttggg gatcataatc gacacccaat
    18901 ccccctgctt cactttcagc gcatccgcca cgcctttgcc gatgataatt tgctgttcgc
    18961 ccgctttaaa attgcgccac gcatcgccct ggacaaacga gggtaatgcg ctcagacgct
    19021 gttcctgttg cgggttaacg cccttcacct ggattgcgcg aagattcacg ccactttcca
    19081 ccagcccggt gaaattgata tacggcgcgg cagcggcaat acccggcact ttttgcacgt
    19141 gatccagtgc ttcctgccag ttagtccacg gctgatccac cgcctcgatt tcaccatgcg
    19201 gcaccaccgc cagaatacgg ttgttcagtt cgcgttcaaa gccgttcatc gcgcttaagc
    19261 cgacgatcaa taccgccacg ccgagggcaa tgccaatggt agaaataacg gagatcagcg
    19321 acaccatgcc gccgcgccgc cgtccgcgac taaaacgcag gccaattaat aacgataaag
    19381 gcatcgccat tactccgccc ccatcaggct cagttccgcc gtcagacgac catcacgcat
    19441 ttctaactgg cggctcatac gtttcgccag ttgcaggtcg tgagtaacca ccaggaaggc
    19501 ggtgccctgc aagcgattca attccccaag caactggaag atgctgtcgg cgttacgcgc
    19561 atcgaggtta ccggtaggtt catccgccag taccaggcgc gggttattca ccagcgcacg
    19621 ggcaatcgcc acacgctggc gttcgccgcc agaaagttca gatgggcggt gattcgcacg
    19681 atgatccagc cccaccgctt ttaacatctc aagtgcacgg ctgttgattt cagcgggctt
    19741 tttcttgcca atcagcagcg gcatagccac gttttccagg gcggtaaaat ccggcagcag
    19801 gtggtgaaac tgataaataa agcccagctt ctggttgcgc agctctgctt tcgccgccga
    19861 agacagtttg ctcatcggtt gaccgttaaa gatcacatcg ccggaggttg gcgtatccag
    19921 cccgcccaac aagtgcagca aggtgctttt accggaacca gagctaccga cgatcgccat
    19981 catttcacct tcgccgacgc tgaaactgac attgtgcagc acatcggttt gcacactgcc
    20041 ttcctgatag cgtttgcaca ggttgtcgca ttgcaacagg atcttattca taacgtaaag
    20101 cctcagcggg ttgagtggcg gcagcgcgcc atgaagggta aagcgtagac agcagcgcga
    20161 tagccatcgc caccagcgca ataacaatga cctgtaaagg ttcgatagcg accggcagcg
    20221 ccgcgccatc aagcaggacg ccgattatcg gcatcagatt atttaactgg ctggcaagca
    20281 gggcgccaag cgccgctccg aggatcgcac cgataatccc ggcgctggcc ccttgcacca
    20341 taaagaccat catgatttgt cgcggagtta agccttgcgt ttgcaggatc gctacttcgc
    20401 cctgcttctc cattaccatc agccctagtg aggtaataat gttaaacgcc gcaacggcga
    20461 caatcaggct cagcagtaaa cccatcatat ttttttccat gcgtacggcc tggaacaact
    20521 cgcctttacg atcacgccag tcctgccatt tgctgccttc aggcagtttt tgctgactta
    20581 atgagtcgac tttcagcggc tcatccagcc acaaacgcca gccggtaata ttgcctgccg
    20641 gataacgcat cagacgcgag gcatcctcaa tattcaccag catttcattg ccatcgactt
    20701 cactgttggc ggcgaaagtg ccaatcacat tgaacaggcg ctgacttgga atacgcccca
    20761 tcggcgtgaa ctggctggca gatggtacca tcatgcggat ttgatcaccg cgattaacgc
    20821 ctagctgtga ggcaagttgt tcgccgagga tgacattata tttccccggc tcgaggtcag
    20881 tttgtttcac attgaccaga tacggcgtta gtggatcttt ttgcgccggg tcgataccga
    20941 gcatcacccc gaccgccacg ctgcgcgcgc tttgcagtac cacatcaccg gtagtaatag
    21001 gtgcgacgcg attaacgccg tccagtttga ctgccgtttc cgggagttgc tgcgggttaa
    21061 gagagccatg ctcagaagag agaattgcct gtggcatcag gccaaggatg ttgttttgca
    21121 gctcgcgctc aaagccgttc atcactgaca atactgtgac cagcgccatc accccgaggg
    21181 taatgccgat ggtagaaagc caggagacga aacgaccgaa gcgatccgct gcacgcccac
    21241 gcatgtaacg caggccaata aatagagcga caggttggta catgaaatcc gtctggttgc
    21301 tgtagcaaag acacgagtat ataagcgaaa gcaagatggg taaacgtctg aactgtaagt
    21361 gatcgtcagg gctaatctga aaagcccccg tgaatcaatc ggctaaattg ccatgtttta
    21421 tcatcaatct tgccgcctga tcttaatctg aaaaatcatc caatacagac agatacccat
    21481 tacacatagg gtcataaccc gcaggatcaa ccgcatgtaa attaaaaaag agcgtgttgt
    21541 cctgatgaaa caaaaagagc tatggattaa ccagatcaaa gggttatgta tttgtcaaac
    21601 catgtcgatt acggataatg aacagcattg cttatcagag atatgccccc atatgttgag
    21661 gcatatccta acgagaatct gacaaccgtt atgcctgaac aatatcgtta tacgctgccc
    21721 gtcaaagcgg gtgagcagcg tctgctgggc gagttaaccg gcgctgcctg cgcgacgctg
    21781 gtagcggaaa ttgccgaacg tcacgccggt ccggtggtac tcattgcacc agatatgcaa
    21841 aatgctctgc gtttgcatga tgaaatcagc cagtttaccg atcaaatggt gatgaatctg
    21901 gcggactggg aaactcttcc ctacgacagt ttttcgcctc atcaggacat tatctcctcg
    21961 cgcctttcca ccctttacca gctaccgacg atgcagcgtg gcgtactgat tgttccggtg
    22021 aatacgctta tgcagcgcgt ttgcccgcac agttttcttc acggtcatgc gctggtgatg
    22081 aaaaaaggtc agcgtctgtc acgagatgca ttaagaaccc aactggacag cgccggttat
    22141 cgccatgttg accaggtaat ggagcacggc gaatacgcca cgcgcggcgc gttgctggat
    22201 ctcttcccga tggggagtga gctgccttat cgtcttgatt tctttgatga tgaaatcgac
    22261 agcctgcggg tgtttgacgt cgacagccag cgcacgctgg aggaagtaga agcgatcaat
    22321 ctgctgcccg cgcacgaatt tccgaccgat aaagcggcaa ttgaactgtt ccgcagccag
    22381 tggcgcgata ccttcgaagt gaagcgcgat ccggaacata tttaccagca agtgagtaaa
    22441 ggcacattac ctgccgggat cgagtactgg cagccattgt tcttcagcga accactgccg
    22501 ccgctgttca gttatttccc tgccaatacc ttgctggtga atactggcga tctggaaacc
    22561 agtgccgaac gtttccaggc tgacacgctg gcgcgttttg agaatcgcgg cgtcgatccg
    22621 atgcgcccgc tgttgccacc acaatcgctc tggctgcggg tggacgagct cttctcagag
    22681 ctgaaaaact ggccccgggt gcagctaaaa actgaacatt taccgacaaa agccgcgaat
    22741 gccaatttag gtttccagaa actgccagac ctggccgttc aggcacaaca aaaagcgccg
    22801 ctggatgcgc tgcgtaagtt cctcgagact ttcgacggtc cggtggtgtt ctcggtagaa
    22861 agtgaaggtc gccgtgaagc gctgggtgaa ctgctcgcac gaattaaaat tgctccgcaa
    22921 cgcattatgc gtcttgatga agccagcgac cgtgggcgtt atctgatgat tggcgctgcc
    22981 gaacatggtt ttgtcgataa ggtgcgtaat ctggcgctga tctgcgaaag cgatctgctc
    23041 ggtgaacgtg ttgcccgccg tcgtcaggat tctcgccgca ccatcaaccc cgatacactg
    23101 atccgtaacc ttgcggagct gcatattggt cagccggtgg tccatctgga gcacggtgtc
    23161 gggcgctatg ccggaatgac cacgctcgaa gcgggcggca ttactggcga gtatttgatg
    23221 ctcacctatg ccaacgacgc caaactgtat gttccggtgt cgtcactgca tctgattagc
    23281 cgttacgcag gtggcgcgga agaaaacgcc ccgctgcata aacttggcgg cgatgcgtgg
    23341 tcacgcgcgc ggcagaaagc ggcggaaaaa gtgcgtgatg tggcggcgga attgctggat
    23401 atctacgcgc aacgcgccgc caaagagggc ttcgcgttta aacacgatcg tgagcagtat
    23461 cagttgttct gcgacagctt cccgtttgaa accacgccgg atcaggcgca ggccattaat
    23521 gcggtactta gcgacatgtg tcagccgctg gcaatggatc gtctggtgtg cggcgatgtt
    23581 ggctttggta aaacagaagt ggcgatgcgc gccgctttcc tggcagtaga taaccacaag
    23641 caggtggcgg tgttggtgcc taccaccctt ctcgcgcagc agcattacga caacttccgc
    23701 gaccgtttcg ccaactggcc ggtacgtatc gaaatgatct cccgtttccg cagcgccaaa
    23761 gagcagacgc aaatccttgc ggaagtggcg gaagggaaaa tcgatattct gatcggtacg
    23821 cacaaactgc tgcaaagtga cgtcaagttt aaagatttag gcctgctgat tgtcgatgaa
    23881 gaacaccgct tcggggtgcg tcataaagag cgtattaaag cgatgcgcgc gaacgtggat
    23941 attctgacgc ttactgcaac gccgatccca cgtacgctga atatggcaat gagcggaatg
    24001 cgtgacctgt cgattatcgc cacgccgccc gcccgtcgtc tggcagttaa aacctttgtc
    24061 cgtgagtatg acagcctggt ggtccgggag gcgatcctgc gtgaaatttt gcgcggagga
    24121 caggtttatt atctctacaa tgatgtggaa aacattcaga aagccgccga acggctggca
    24181 gaactggtgc cagaagcgcg gatcgccatc ggtcacgggc agatgcgcga gcgcgaactg
    24241 gaacgggtga tgaatgattt ccatcatcaa cgtttcaacg tgctggtttg tacaaccatt
    24301 atcgaaaccg ggatcgacat cccgacagcc aacaccatta tcattgaacg cgcggatcac
    24361 ttcggtctgg cgcagctgca ccagttacgc ggtcgcgtcg gacgttcaca tcatcaggca
    24421 tatgcatggt tgctgacgcc gcatccaaaa gcgatgacta ccgatgcaca aaaacgtctt
    24481 gaagcgattg cctcgctgga agatctcggg gcaggttttg cgctggcaac gcacgatctg
    24541 gagattcgcg gcgcgggtga actgcttggc gaagaacaaa gcggctcaat ggaaaccatc
    24601 ggtttctcgc tgtatatgga gttgctggaa aacgccgtcg atgcactgaa agccggacgc
    24661 gagccgtcgc tggaagatct caccagccag caaacagaag tcgagctgcg gatgccgtcg
    24721 ctattgccag atgatttcat tcctgacgtg aatacgcgtt tgtcgttcta taaacgtatt
    24781 gccagcgcca aaacggaaaa cgaactggaa gagatcaaag tcgagcttat cgatcgcttc
    24841 ggcctgctgc cggatccggc gcgtaccctg ctggatgttg cccgtctgcg ccagcaagcg
    24901 cagaaactgg ggatcaggaa gctggaaggt aatgagaaag gcggggtgat cgaatttgcc
    24961 gagaagaatc acgttaatcc ggcctggttg attggtttgc tgcaaaaaca gccgcagcat
    25021 taccgccttg atggtccgac gcgcctgaaa tttattcagg atttgagtga gcggaaaacg
    25081 cgtatcgaat gggtacgcca gtttatgcgt gaactggaag agaacgcgat cgcttaatgg
    25141 taaatcgggg gcgtttctgc gcccccatct gcaacattta caaattcttt gcacttccct
    25201 gcactatccg acactgtcac catccataat tcaggcttat ctgtttatta caataacctt
    25261 atatttatta tggatttttg gtgatgatca aaacgcgttt ttctcgctgg ctaacgtttt
    25321 ttacgttcgc cgctgccgtg gcgctggcgc taccggcaaa agccaacacc tggccgctgc
    25381 cgccagcggg cagtcgtctg gttggcgaaa acaaatttca tgtggtggaa aatgacggtg
    25441 gttctctgga agccatcgcc aaaaaatata acgtcggctt tctcgctctg ttacaggcta
    25501 accctggcgt tgatccttac gtaccgcgcg cgggtagcgt gttaacgatc ccgttgcaaa
    25561 ccctacttcc agatgcgccg cgcgaaggca ttgtgatcaa tattgcggag ctgcgtctct
    25621 attactaccc gccgggtaaa aattcggtaa ccgtgtatcc aataggtatt ggtcagttag
    25681 gtggtgacac gctgacaccg acaatggtga ccaccgtttc agacaaacgt gcaaacccaa
    25741 cctggacgcc aacggcaaac atccgcgccc gttataaagc acagggaatt gagttgcctg
    25801 cggtagtgcc ggctggaccg gataacccaa tgggccatca tgcgattcgt ctggcggcct
    25861 atggcggcgt ttatttgctt cacggtacga acgccgattt cggcattggc atgcgggtaa
    25921 gttctggctg tattcgtctg cgggatgacg atatcaaaac actctttagc caggtcaccc
    25981 caggcaccaa agtgaatatc atcaacactc cgataaaagt ctctgccgaa ccaaacggtg
    26041 cgcgtctggt tgaagtacat cagccgctgt cagagaagat tgatgacgat ccgcagctgc
    26101 tgccaattac gctgaatagc gcaatgcaat catttaaaga tgcagcacaa actgacgctg
    26161 aagtgatgca acatgtgatg gatgtccgtt ccgggatgcc ggtggatgtc cgccgtcatc
    26221 aagtgagccc acaaactctg taaatcctgg ataaaaaaaa tccccgcgac acggcatgtc
    26281 gcggggaaaa aatcagcagt agcaggcatt aatgagggtt aatgcttatt tataaattac
    26341 tgctgttcca tggagggtat tcggaccggt tacagaagta atacggaaag attttgcgcc
    26401 catctcatcc gctttttgcg ccagctgctc ttccagcgat cccagatttg tccccgcgtt
    26461 agcactgatt gtaccgactt tttgttggcc ttctggcgtt gactgaactt cgacagccgc
    26521 aaagctggca aatgacatgg agcttaaaat cgccgcagcg atgagggttt ttacgttttt
    26581 cataatagtg gccttatgca gatgaatgac gtcgcaagca attacttaac gatcgataac
    26641 taaatgatag atgtgatctg gatcacatac aagatatttt ttataacaat cattaatt
//
LOCUS       DAZFQD010000064        26085 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.064, whole genome shotgun sequence.
ACCESSION   DAZFQD010000064 DAZFQD010000000
VERSION     DAZFQD010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26085
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.064"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(76..465)
                     /gene="mutT"
                     /locus_tag="ACHKTW_002686"
     CDS             complement(76..465)
                     /gene="mutT"
                     /locus_tag="ACHKTW_002686"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HFO6463684.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGEPWGKEGQPG
                     EWMLLVGLNADDFPPANEPVIAKLKRL"
     gene            complement(525..3230)
                     /gene="secA"
                     /locus_tag="ACHKTW_002687"
     CDS             complement(525..3230)
                     /gene="secA"
                     /locus_tag="ACHKTW_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFO6463685.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLEAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(3292..3804)
                     /gene="secM"
                     /locus_tag="ACHKTW_002688"
     CDS             complement(3292..3804)
                     /gene="secM"
                     /locus_tag="ACHKTW_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secA translation cis-regulator SecM"
                     /protein_id="HFO6463686.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLT"
     gene            complement(4035..4952)
                     /gene="lpxC"
                     /locus_tag="ACHKTW_002689"
     CDS             complement(4035..4952)
                     /gene="lpxC"
                     /locus_tag="ACHKTW_002689"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HFO6463687.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(5053..6204)
                     /gene="ftsZ"
                     /locus_tag="ACHKTW_002690"
     CDS             complement(5053..6204)
                     /gene="ftsZ"
                     /locus_tag="ACHKTW_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFO6463688.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(6265..7527)
                     /gene="ftsA"
                     /locus_tag="ACHKTW_002691"
     CDS             complement(6265..7527)
                     /gene="ftsA"
                     /locus_tag="ACHKTW_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFO6463689.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(7524..8354)
                     /gene="ftsQ"
                     /locus_tag="ACHKTW_002692"
     CDS             complement(7524..8354)
                     /gene="ftsQ"
                     /locus_tag="ACHKTW_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HFO6463690.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLGWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLARFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(8356..9276)
                     /gene="ddlB"
                     /locus_tag="ACHKTW_002693"
     CDS             complement(8356..9276)
                     /gene="ddlB"
                     /locus_tag="ACHKTW_002693"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFO6463691.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSMDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQELVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(9269..10744)
                     /gene="murC"
                     /locus_tag="ACHKTW_002694"
     CDS             complement(9269..10744)
                     /gene="murC"
                     /locus_tag="ACHKTW_002694"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFO6463692.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLMNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(10798..11865)
                     /gene="murG"
                     /locus_tag="ACHKTW_002695"
     CDS             complement(10798..11865)
                     /gene="murG"
                     /locus_tag="ACHKTW_002695"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFO6463693.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHHLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPLLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(11862..13106)
                     /gene="ftsW"
                     /locus_tag="ACHKTW_002696"
     CDS             complement(11862..13106)
                     /gene="ftsW"
                     /locus_tag="ACHKTW_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HFO6463694.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSSVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(13106..14422)
                     /gene="murD"
                     /locus_tag="ACHKTW_002697"
     CDS             complement(13106..14422)
                     /gene="murD"
                     /locus_tag="ACHKTW_002697"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFO6463695.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDAECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALAA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     QVDGTLHLLLGGDGKSADFSPLARYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(14425..15507)
                     /gene="mraY"
                     /locus_tag="ACHKTW_002698"
     CDS             complement(14425..15507)
                     /gene="mraY"
                     /locus_tag="ACHKTW_002698"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFO6463696.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(15501..16859)
                     /gene="murF"
                     /locus_tag="ACHKTW_002699"
     CDS             complement(15501..16859)
                     /gene="murF"
                     /locus_tag="ACHKTW_002699"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFO6463697.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGTEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSVGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQLITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(16856..18343)
                     /gene="murE"
                     /locus_tag="ACHKTW_002700"
     CDS             complement(16856..18343)
                     /gene="murE"
                     /locus_tag="ACHKTW_002700"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HFO6463698.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKDEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVEQGATFCAMEVSSHGLVQHRVAALKFAASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKA
                     IDVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGVIA"
     gene            complement(18330..20096)
                     /gene="ftsI"
                     /locus_tag="ACHKTW_002701"
     CDS             complement(18330..20096)
                     /gene="ftsI"
                     /locus_tag="ACHKTW_002701"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HFO6463699.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNPDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(20112..20477)
                     /gene="ftsL"
                     /locus_tag="ACHKTW_002702"
     CDS             complement(20112..20477)
                     /gene="ftsL"
                     /locus_tag="ACHKTW_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFO6463700.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(20474..21415)
                     /gene="rsmH"
                     /locus_tag="ACHKTW_002703"
     CDS             complement(20474..21415)
                     /gene="rsmH"
                     /locus_tag="ACHKTW_002703"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFO6463701.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEERF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(21417..21875)
                     /gene="mraZ"
                     /locus_tag="ACHKTW_002704"
     CDS             complement(21417..21875)
                     /gene="mraZ"
                     /locus_tag="ACHKTW_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFO6463702.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLSSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
     gene            complement(22477..23481)
                     /gene="cra"
                     /locus_tag="ACHKTW_002705"
     CDS             complement(22477..23481)
                     /gene="cra"
                     /locus_tag="ACHKTW_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009750 - response to fructose [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HFO6463703.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            complement(23563..23649)
                     /locus_tag="ACHKTW_002706"
     CDS             complement(23563..23649)
                     /locus_tag="ACHKTW_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HFO6463704.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(23661..24152)
                     /gene="ilvN"
                     /locus_tag="ACHKTW_002707"
     CDS             complement(23661..24152)
                     /gene="ilvN"
                     /locus_tag="ACHKTW_002707"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFO6463705.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIREVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            complement(24155..25879)
                     /gene="ilvI"
                     /locus_tag="ACHKTW_002708"
     CDS             complement(24155..25879)
                     /gene="ilvI"
                     /locus_tag="ACHKTW_002708"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HFO6463706.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVWPESVSMRSYNPTTTGHKGQIKGALQTLVAAKKPVVYVGGGAI
                     TAGCHQQLKETVEALNLPVVSSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRARQCLKYDTHSEKIKPQAVIETLWRLTNGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHELESKLSEALEQVRNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            25998..26042
                     /locus_tag="ACHKTW_002709"
     CDS             25998..26042
                     /locus_tag="ACHKTW_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6463707.1"
                     /translation="MKRNDKILHYEGLN"
BASE COUNT         6163 a         7492 c         6308 g         6122 t
ORIGIN      
        1 cctgataaga cgcggacagc gtcgcatcag gcatcttgtg caaatgtcgg atgcggcgtg
       61 aacgccttat ccgacctaca gacgcttaag cttcgcaatt accggttcat tggctggcgg
      121 aaaatcatcg gcattaagac cgactagcaa catccactca ccgggttgcc cttctttacc
      181 ccacggctcc ccttcccagc tttcgaccag ccagaaccac agagttatat gcctgtccgg
      241 gaattcatat tccagttttt caaatagcga aaaatgttgt ggggtaatcc cgacttcttc
      301 ctgaagttca cgcaccaccg cctgttccgg cgtttcgccc atttcaattt taccgccggg
      361 aaactccagt ttattcgcca tgtgcgcatc tgctgcgcga cgcgttataa agatttcatt
      421 gttctcgttg cgaataatac ctaccgcaat ttgcagcttt ttcattgtct taaacctata
      481 aaaaaggcgc agaatcctgc gccttttact tcaactgtta gcttttattg caggcgacca
      541 tggcactgct tgtatttttt accagaaccg cacgggcaag gatcgttacg tcctactttg
      601 cgctcaccgg tttgcgccgc cagtgcagct gcggctgcag agtcgtcatc ctgatggcta
      661 agctgctgca tttgcgctaa acgctcggct tccatacgac gctgttgttc cagctcctca
      721 acctcttcag gcatacgtac ctggactttg ctcagcgtgc tgataacttc atattttaac
      781 gactccagca tcgctgcaaa catggagaac gattcacgtt tgtattcctg cttcggatct
      841 ttctgtgcat agccacgcag gtggataccc tgacgcagat agtccatcgc cgccaggtgc
      901 tctttccaca gagagtcgag agtttgcagc atgacgcctt tttcgaagtg acgcatcatc
      961 tcagcaccaa ccacttcttc tttacgctga tacacttcga tggactgcgc cagaattcgc
     1021 tcacgcagcg tctcttcatg cagttctggt tctttatcca gccactcggc aattggcaaa
     1081 tcgaggtcga aatcgttctt cagacgttcc tgcagccccg gaatatccca catttcttcc
     1141 agtgactgtg gcggaatgta ggcatcaatg gtcgctttga acacatcttc acgaatgctg
     1201 ttgatggttt cgctcacatc gctgacatcc agcagttcgt tacgctggga gtaaatggcg
     1261 cgacgctgat cgttagccac gtcatcatat tccagcagtt gcttacgaat gtcgaagtta
     1321 cggctttcaa ctttacgctg ggcgttggca atcgctttag tcacccacgg gtgttcaatg
     1381 gcttcgcctg gcttcatacc cagtttacgc atcatgccgg atactcggtc ggaagcaaaa
     1441 atacgcatca gcgcatcttc catcgacagg tagaaacggg aagaaccagc atccccctga
     1501 cgaccagaac gaccgcgcaa ctggttatcg atacgacggg attcgtgacg ctcggtaccg
     1561 atgatatgca ggccacctgc ttccagtacc gcatcgtgac gtacctgcca gtcggcttta
     1621 attttttcaa tttgctctgc ggtcggattt tccagcgcgg caacttctgc ctgccagcta
     1681 ccaccgagca caatatctgt accacgaccc gccatattgg tcgcgatagt caccgcagcc
     1741 ggataacctg cctgagcaac aatcgccgct tcgttggcgt ggaatttggc gttcaggacg
     1801 ttgtgcttaa taccggcttt ggtcagttcg tttgacacca gctccgattt ttcgatggag
     1861 atagtaccca ccagcaccgg ctggcctttc gcagtacgtt ctttgatatc ttcaatgatc
     1921 gcctgaattt tttccgcttc agtcatgtag accaggtccg gcagatcttt acgaatcatt
     1981 ggacggttgg tcggaacaac gacggtatcc agcttgtaga tggagctaaa ttcgaaagct
     2041 tcggtatcag cagtaccggt catccccgcc agtttttcat acagacggaa gtagttctgg
     2101 aaggtgatcg aagccagcgt ttggttttcg ttctggatct gcacaccttc tttcgcttcc
     2161 acagcctggt gcagaccatc ggaccagcga cggccctgca tggtacgacc ggtgtgttcg
     2221 tcaacgatga taacttcacc atctttaacg atgtagtcga cgtcgcgggt aaacagcgca
     2281 tgagcgcgca gcgccgccgt tacgtggtgc atcagcatga tgttggccgg agagtacaga
     2341 gactcccctt catccatgat gccctctttc accagcagtt cttcaatcag caccagacca
     2401 cgttcggtca ggttcacctg gcgagatttt tcgtccaccg agaagtggcc ttcgccctgg
     2461 aaggtttcgg agtcttcttt ttcctgacgg atcaggtgcg gaataatttt attcacgcgt
     2521 ttatacattt ccgagctgtc ttctgccggg ccggaaatga tcagcggtgt acgcgcttca
     2581 tcgatcagga tggagtccac ttcgtccacc agcgcatagt gcagtttacg ctgtacacgt
     2641 tcttcagggc tgaacgccat attgtcgcgc aggtagtcaa agccgtattc gttgttcgta
     2701 ccgtaagtga tgtcagctgc gtaagcttcg cgctttgccg gtgctggcat gcccggcagg
     2761 ttgataccga cagtcaggcc aaggaattca aacagcggac ggttgttttc ggcgtcacgt
     2821 tgcgccaggt agtcgttgac ggtaactacg tgcacgcctt taccggttag tgcgttcagg
     2881 taagcaggca gcgttgcggt cagggtttta ccttcaccgg tacgcatttc ggcgatgcag
     2941 cgttcgttaa gaaccatacc gccgagtaac tgaacgtcga agtgacgcat accaaagacg
     3001 cgcttacttg cctcacgtac cacggcgaaa gcttccggga tcagattttc cagcacttcg
     3061 cctttttcca gacgtgcacg aaactctgcg gttttccctt tcagttcttc gtcggagagt
     3121 ttttccatct ccggttccat ggcattgatg atgttgacca ctttgcgcat ccggcgcagg
     3181 gtgcgatcgt tacgactacc gaaaacttta gttaacaatt tgattagcat aataaaatct
     3241 caaacgcccc gcgttgcgga gttaataaaa tgaagtaaag gtttattgtt gttaggtgag
     3301 gcgttgaggg ccagcacgga tgccttgcgc ctggcttatc cagacgggcg tgctgaattt
     3361 tgcttgtggg gtaaaatgcg cataatcaat gcgataaccc ttttcagacg gcgtgccttc
     3421 ctgggtcagc agcgcgctga gcgtatccag taatgcaaga tgttgcgcct gaagaggcaa
     3481 agattcttca gcaacgggca gtgtttgcgg tgccattgcg aaagaaagat gacggattac
     3541 cgtgcgaatg gcatgttgat gccagtaatc aacggaatag ttcgaattcg ggcggcgtgt
     3601 gttcgcttcc agcaaggcca attgaccaaa gttaactttg gctgaaggct cgtggttgcg
     3661 ggttgtcgct tttgcgggcg cgtttggttc ggcggcgttg ctgagcgcag gcaaacctaa
     3721 actcgccgca accatcccta ataagagatg cggccagaag tagcgtttac caaactgtcg
     3781 ccagcgcgtc agtattccac tcacgttatt gccatcccga tcatattcaa acgtatttag
     3841 cgcacaaatc ttatcattaa agcctgcagt ccacagcacg aatgaagata agttgtgcgg
     3901 aaaagtgctt aagaaaacag cgttcgcacc gttttttata gctcttataa aattcaaaaa
     3961 aaaacgactg gttcacctgg ccggagagag tgccagattt gccagtcgaa ttttatacga
     4021 cagtataaat gtcgttatgc cagtacagct gaaggcgctt tgaaggccaa cggcagttct
     4081 gcgtcgtcct ggaaggtcac atattcccag gcttcctgtt tcgccaggac agcctgcagc
     4141 agtttgttat tcagtgcatg accggattta taagcggtaa atgcaccaat aatattgtga
     4201 ccacacatga acaagtcacc gatcgcatcg agcattttgt gacgcacaaa ttcgtcttca
     4261 aaacgcaggc cgtcttcgtt cagtacgcga taatcgtcaa caacgatggc acaatcgaag
     4321 ctgccgccca ggcacaaacc acgggactgc agatattcga tatcacgcat gaaaccgaac
     4381 gtacgcgcac ggctgatctg gcgcataaac gcatcagcgg agaagttcat cgcatagcgc
     4441 tggttgctgg aatcaatagc cggatggtta aaatcgatgg tgaaatccag cgaaaaacca
     4501 ttgtacggct taaattcagc ccacttatcg ccatcttcga cacgaacagt ctctttgatg
     4561 cgaacaaatt ttttggcgca gttcaactcg tcgataccgg cgtcaagcag caggtataca
     4621 aacggagcgg cgctgccgtc catgatcggg atttccggcg cgttaacttc gataacaatg
     4681 ttatcgatgc ccaagcccgc gagagcagca ttgaggtgct ctacggttga aatccgtaca
     4741 tcatgctcgt tgaccagaca cgtacagagc atggtatcac gcacagattt ggcatcggcc
     4801 gggaaatcta ccggtggatt caagtcggtg cgacgataga tgaccccggt gttggccggc
     4861 gcagggcgta acgtcagggt gactttcttg ccggtatgta aaccgacacc cgtcgcctga
     4921 acgatacgtt taagtgtcct ttgtttgatc atcgtattat ctcgccaaat tacctatcca
     4981 accgaagtgt actatacatt cggcgggcca gtttagcaca aagagcctcg aaacccaaat
     5041 tccagtcaat tcttaatcag cttgcttacg caggaatgct gggatatcca gataatccgg
     5101 ctctttcgca gtttgcggcg cattgtcatt cacgacttta gcaaccggct tctgctcctg
     5161 ggtcagcgga gccatcccat gctgctggta gcgatccatc actggctgct gaacctgctt
     5221 attggtcacc agagtgattt caggacgttt gtccatgccg atacctgtcg caacaacggt
     5281 tacgcgcagc tcgtcattca tatccgggtc aagagaagta ccgataacca cagtcgcgtt
     5341 gtcggaagca aatgcacgga tggtgttacc taccgtttcg aactcatcca gacgcaggtc
     5401 gaagcccgcc gtgatgttaa ccagcacgcc gcgcgcgcca gacaggtcga tatcttccag
     5461 cagcggagaa gagatagcca tttcagcagc ttcttccgca cggtcttcac cgctcgccac
     5521 gccagaaccc atcattgcgt agcccatctc agacattacg gtgcgtacgt ctgcaaagtc
     5581 cacgttcatc aaacccggac gagtaatcag ttcagcgata ccttgcacag cgcctttcag
     5641 tacatcgttc gctgcgccaa acgcatccag cagggagata ccgcggccca gaactttcag
     5701 cagtttgtcg ttcgggatag tgatcagaga gtccacatgc ttggacagtt cagtgatccc
     5761 ctgctccgcg aatgccatac gcttcttgcc ttcaaagttg aaaggcttag tgacgacagc
     5821 aacggtcagg atacccaaat cttttgccac ttcagcgacg actggtgctg cacctgtacc
     5881 ggtaccacca cccatacccg cagcaataaa gaccatgtct gcaccttcca gcgccgcacg
     5941 caatgcatcg cgatcctcat cagccgcatt gcggccaact tctggattag cgccagcgcc
     6001 cagtcctttg gtgataccgc taccgatttg aatcgtctgt ccaaccgctg ttttacgcag
     6061 cgcttgtgca tcggtattta ccgcgaagaa ttcaacacct tcaatgcgct cgcgcaccat
     6121 gtgttcaaca gcattaccgc cgccgccgcc gacgccgatg actttaatca ccgcgtcatt
     6181 ggtaagttcc attggttcaa acatagtttc tctccgattt gtgcctgtcg cctgaggccg
     6241 taatcatcgt cggcctcata aaaattaaaa ctcttttcgc agccaactat tgagtcgctt
     6301 gatccacgag ccaactgatg ctgtaacacg tttttctact tcagcttcac cgttaagatg
     6361 tgactctttc ccatagtgaa gcaatcccac cgccgtcgaa taatacggct cctgagcata
     6421 atccgttaaa ccggtaatgt tcagcggcgc gccgatacgc acttgcgtat gaaacacgcg
     6481 ctgagcacag gctgcaagac cttcgatctg cgctgcgcca ccggttaata caatgcctgc
     6541 cgccaggtga tgtttaaccc cttgttggcg aagcttttcc tgcaactgca atatctcttc
     6601 gttgaccagg ttgagcagct cggtatagcg cggctcgatc acctctgcca gggtctgacg
     6661 ttgcagactc cgcggcggac gaccacctac gctcggcact tccacgctct catcttttcc
     6721 aacgatggaa cccagcgcac aaccgtggcg aactttaatc gcttcggcgt cgcttggcgg
     6781 cgtgccaaag gcataagcga tatcactggt cacgacattg ccagcataag gaattacctt
     6841 agtgtggcgc aatgccccac cggtataaac ggcgatatcc attgtaccac caccgatatc
     6901 gacgacgcag acacccagtt cacgttcatc ttccgtcaat accgaataac ttgatgccag
     6961 tccggcaaat atcagttggt caactttcag cccacaacgt tcaaccgctt tgacgatgtt
     7021 tttcgccata tcgttgtgac atgtgatcag gtgcactttt gcctgcatcc gcacgcccga
     7081 aagtcctacc ggattcttga tcccttcctg atagtcaatc gcatactctt gcgggatcac
     7141 atgcagcaca cgatgctcat cgcgcacacg caccgatttc gcggtatgga cgacgttttc
     7201 cacatcttct tgcgtcactt cttcttcaga aataggcacc ataccaattt cattctggca
     7261 gctgatgtgc ttaccagaaa gcgccagata taccgaagag atctgacaat ctgccatcaa
     7321 ttctgcctgg tcaatggcgc gttgtacgca cttgaccacg gattcgaggt cgttcacccc
     7381 gcctttatcc ataccacgtg acgggcagct gcccacgcca atgatattga ccataccgtc
     7441 gggcagaact tcccctacta aagcggcaac cttcgcggta ccaatctcca gtcctactac
     7501 cagttttctg tccgtcgcct tgatcattgt tgttctgcct gtgcctgatt ttgttgctga
     7561 gtagattcct ctggcggcaa gggcgcccag cctactgccg ctccagagtc ataacgcaaa
     7621 tcaacgtagc taatccgttt gccatcggtt tgcgcctgct gctgtaaaac cggataaagt
     7681 tctacaaagc gagccaaacg tttcatcgta tcgccccggc caagattgag cttaatatcg
     7741 ttattcagcg tcaactgcca ggaacgccgc gcggtcatcg ccgcttcctt cagagtaaat
     7801 ctgtcctttg ccagcatctg ccccatttcg cgatagccct gcaacacttc attggcgctg
     7861 ccttccgggc catacagcat tggaagcacc tgcttgctgg tgcgatctgg cggcacgctg
     7921 aaggtgtttc cttccgcgtc taccatatgt tgatcattcc accgcgcaat cggcacatat
     7981 tcaaccagat gaatcttcaa ttcatcaggc cactgctttc tgacgctcac ctgcttaatc
     8041 cacggcaggc gttgttctat ttgcgtctgg atgatgttga catcttgggt cataaaggta
     8101 cccggctcac ccaatgccag gatcgactgc cggatatcgt cattacgcgt gtaatggcgt
     8161 tcaccggtca acaccagctt tgagagcggc aggcgttgcg catcttccat ccagcccaac
     8221 acgacccagc cgctcaccaa cactgtcgtt aaaacggtca gcaggaaaag gatccccgcc
     8281 agacgcgttc cattattgcg gcgagaagaa acctcttctt cgctgtttcg cgtgttcaga
     8341 gcagcctgcg acatattagt ccgccagttc cagaattcgt actaccaact gcgagaagct
     8401 catacctgcc tgacgtgccg ccatcggcac caggctgtgg ctggtcatac ccggtgaggt
     8461 attggcttcc agcagataaa actgtccatc gctgtccagc ataacgtcaa tacgtcccca
     8521 tcctttgcaa cctaacgtcg tccatgcttt cagcactaat tcctgcaaat tggcctcttg
     8581 tgacgcttcc agacctgcgg ggcagaaata ctgtgtctca tcagagagat acttcgcctc
     8641 ataatcatag aaggttccgg acggttgaat acgtattgac ggtaaaattt cttcaccgag
     8701 tatcgcaacc gtgaactccg gcccacttag ccatttttca atcaatactt cttcatcgtg
     8761 ctgaaatgcc aatcttaatg catcttgtag agcattttct gctactactt ttgacattcc
     8821 cacactggaa ccttcgcggc tcggcttaac gataaccggc aaacccagag cagaaatttc
     8881 tgctaactgc ttatcactca ggcctttttc aaactctgcg cgggttaacg caacccacgg
     8941 agcgaccggt aaaccggcac cttgccacag cagtttgctg cgtagtttat ccattgaaag
     9001 cgcagatgcc atcactccac tgccggtata aggcaagccc atcagctcga gcatgccctg
     9061 cagcgtacca tcttcaccgc cgcgaccgtg tagcgcgata aacactttct gaaagcccat
     9121 cgacttcagt tgcgtcacgt cgacttcttt cgggtcgaca ggatacgcat caataccgcc
     9181 ttcacgcagc ccggctaaca ccgctgcgcc agaattcaga gaaacttccc gctcagcgga
     9241 agtcccgccc aacaggaccg cgattttatc agtcatgttg ttcttcctcc ggagtttgcg
     9301 gcttcagttt gatttcagct aaagaacggg caatttttcc gatattacca gccccctgaa
     9361 cgagaatcag gtcgttaccg gttaataccg gtgccagcat ctcggctacc tgcgcagggt
     9421 ccggcaccag aatgggatca attttcccac gtccacgaat tgtgcgacac agcgaacggc
     9481 tgtccgctcc cggaattggc gcttcgccag ccggatacac ttccagcatc aacagggtat
     9541 caacctgcgt cagcacattg gcgaaatcat catacaggtc gcgcgtacgg gtaaaacggt
     9601 gcggctgaaa cagcattacc aggtttttat ccggccagcc tgcgcgcgcc gctttaatgg
     9661 tggcgtcaac ttccgtcggg tggtggccgt agtcatcgac cagcattgcc gtaccgcttt
     9721 taccattcac tggctccagc gggaattcac cgaggaaatc aaaacggcga ccagtcccct
     9781 ggaagctctc cagcgcacgc aaaatagcct cgtcgtcaat tccctcttcc gtagcaaccg
     9841 caaccgcagc tgcggcgttc agcgcgttat gacggcctgg cgcattcagg gtgacgcgca
     9901 tcggctcttt gtcctggcgc agcagcgtaa agtgcccctg cgggccaatc tgctgataat
     9961 cttctacacg cacgtcggca tcttcgctga agccgtaagt tgtggtctga cgtcccacac
    10021 gcggtaacaa ttcgcggatc accggatcat caacacacat caccgcacga ccgtaaaaag
    10081 gcaggttgtg cagaaaatta ataaaggtct gttttaaatt ctcaaagtcg ccctggtagg
    10141 tatccatgtg gtcggcttcg atattggtga caatcgccac catcggttgc agatgcagga
    10201 acgatgcatc actctcatct gcttcggcaa tcaggtaccg accatgcccc aaacgcgcat
    10261 gaacccccgc cgcttttacc agcccgccgt taacgaaggt tgggtcgagc cccgcttctg
    10321 cgtagatgct ggaaaccatc gcggtagttg tcgttttgcc gtgcgttcct gcaatggcga
    10381 tgccatgacg aaaacgcatt aactcagcca gcatttcggc acgacggatc accggaatac
    10441 gcgcttcatg agctgcgaca atttccgggt tatcggcaga aatcgcgctg gaaacaacaa
    10501 ccacgctggc atcacgtacg ttttccgggc gatggttgaa ataaatcgtc gctcccagat
    10561 tcattaactg ctgcgtgacc ggatttggcg ctaaatcgga accactgatc tgataacctt
    10621 cattggccag aacttcggca ataccgccca taccggcacc accgatgccg acaaaatgta
    10681 tgtgccgaac gcgacgcatt tcgggcacga tggaacgcag ttttgccaat tgttgtgtat
    10741 tcattcttta cgccattaac ttcttaaatt catacgatgc aaaagacatc gctacaatta
    10801 cgcccgggca gcccggctca cttcatttgc cactcgctcg gtggcatccg gaatggatgc
    10861 agcgcgggcg cgttctgcca tggttaataa ggtttctcgc gaccacccgg ccagggtgtt
    10921 ggcgacagca tccacgctaa gcagtggctg ctcgataatt ttggctgcgc ccgctttttc
    10981 cagcggtagc gcattccagt attgctggcg gtctttatgt tgaaacggca caaacaacgc
    11041 cggtagtcct gccgcggcga tttcactcac cgttaaagca ccggagcggc aaacgacgac
    11101 atccgcccac gcatacgccg ccgccatatc atcaataaat tcagtcactt tatgctgcgg
    11161 ttgccccgct tcggcatacg cctgttcaac ggattgttgc gaacctttgc cgctctgatg
    11221 ccagatagtg actgaatcac ccagtttcgc agcaacctgc ggcattgtct ggttaagaat
    11281 gcgtgcgccc tgggaaccac ccactaccag cacacgaacc ggaccttcac gtccagccaa
    11341 acgttgctgc ggcaacggta gcgccaacac atcggtacgc accgggttac ccaccacttc
    11401 cgcattgggg aaagcacctg gaaacgcctg catcactttg gtggcaatct tcgccagcca
    11461 tttattggtt aagcccgcaa taccgttttg ttcatgaagt acaaccggaa tgcctaacga
    11521 ccacgcggcc agaccacctg gacctgatac atagccgccc ataccgagta ccacgtcagg
    11581 tttgtacgct ttcataatcg cccgcgcctg acgccaggcg ttgaagatac gcagcggcgc
    11641 agctatcagt gcttttatac cttttccacg cagaccagag atacgaatga aatcaatttc
    11701 gatgccatgt tttggcacta agtccgcttc catacggtcg gcagtcccca gccagcgaac
    11761 ttgccaaccc tgagccatta gatggtgcgc aaccgccagt cccgggaata catgtccacc
    11821 ggttccgcct gccatcacca ttaatcgctt tccttgacca ctcatcgtga acctcgtaca
    11881 aacgcctgcg ctttctccag acgcgtttca taatcaatac gcaacagcat catgatggct
    11941 gtcgacataa tcagtaagct cgaaccaccg taactgatca gcggcaatgt cagacctttg
    12001 gtcggtaaca tccccgccgc cgcgcctacg ttaaccagcg cctggaagct aaaccagatg
    12061 ccaatagaac aggcgagaaa accggaaaaa cggtggtcaa tttctaacgc tttgcggcca
    12121 atcgacatcg cgcgaaaagc gacgaagaat accattaaaa gtgccagcac cacaccgaca
    12181 taccccagtt cttcgccgat aatggcgaaa ataaagtcag tgtgcgcttc cggcagatac
    12241 tccagttttt gtaccgagtt acctaaacct tgtccccaaa gttcgccgcg accaaacgcc
    12301 atcagcgatt gcgttaactg atagccgctg ccaaagggat cttcccacgg gttccagaat
    12361 gcggtaacac ggcggatacg gtacggttcg gcgagtatca gcaacacaac cgctgaaatg
    12421 cccataccga taatggcaat gaactgccac aatttcgctc ccgccaggaa caacatcgcc
    12481 agcgtagtca caaacaacac caccaccgta ccaaggtctg gctgtgccag cagtaacact
    12541 gccaacacca gaatcacgcc catcggtttc aggaagccgc gcaggttatt acgtacttcg
    12601 tcgcctttac gcaccagata gttggcgata taacaaaaca gcgacagttt tgtcagctcc
    12661 gcaggctgga tacgcagcaa accgagatcg atccaacgcg atgccccttt aaccgagcta
    12721 cccactacca gcacgatcat cagcaggatg atagatccga gcagcatcgt ggcactgtag
    12781 cgttgccaga actccatcgg cagacgcagc gtaatgatcg ccagaatgaa tgccaggatc
    12841 agatagacgc catcacgctt cgcgaagaag aacggatcgt tggttaagcg ttgccctatg
    12901 ggcattgacg ccgaggtcac catgataaag ccaatcgccg caaggccgaa ggtcagccac
    12961 agtaaggtgc gatcgtacat gatcaggctg tcggtatctt tttcccgcga gcccatcacc
    13021 cagcctttta gcgccgtgga gatccagacc aggatactga atcctggcag gcgcggcatt
    13081 ttcaggcgag ggagagataa acgcatcaac ctaactcctt cgccagacgg gcaaactcat
    13141 tgcctcgttg ttcaaagttc ttgaactgat caaggctggc acaggccggg gagagcagaa
    13201 ccatatcgcc cggctgaaca cgcggagcca gcaagcgcat cgcctgttcc atagtttcgg
    13261 tttgttctgc cacttccggg cgtagcgccg ccagctgcgc accgtcacga ccaaaacagt
    13321 acaaacgcac gttgtcgcca ttcagatagc gcgccagtgg actgaagtcc gccgatttac
    13381 catcgccgcc cagcaacaaa tgcagcgtgc cgtctacctg caagccattc aacgccgctt
    13441 ccgtactgcc gacgttagtg gctttcgaat cgttaatcca gcgtacgcca ttgtgctcca
    13501 gcaccacttc aaagcgatgc ggcagaccgg tgaaggtggt taacgctttc aggctgctgg
    13561 cgcgcggcaa accagctgcg tctgccagtg ccagtgctgc cagcgcattg gtgtagttat
    13621 gctgcccgga aagtttcatc tctttcacat tcagcacttt ctcgccttta acccgcagcc
    13681 aggtttcgcc ctgctgatga ttcaggtgat agtcacccat gttgacgcca aagctgacgc
    13741 agcgttcatc cgcaccgcga atcggcattg ttaaggcatc atcagcatta accacgcaaa
    13801 ctttcgcgtt ttcgtaaatg cgcagttttg ctgcacgata ctgttgtaaa ccaaacggat
    13861 agcgatccat atgatcttca gtcacgttca gaatggtcgc tgctaccgcc tgtaagctgg
    13921 aggtggtttc cagctggaag ctcgacagtt ccagcacgta cagttcgcac tcagcatcaa
    13981 gcagcatcaa cgctggcagg ccaatattgc cacccacacc aacgttaacc cccgccgctt
    14041 tcgccatttc acccactagc gtggtgaccg tgcttttgcc gttagaaccg gtaatcgcca
    14101 caatcggtgc ttgtgcttcg cgacagaaca gctcgatatc gccaacgatt tcgattccgg
    14161 catcagcggc agcgcttaag gatggatgcg ccagtgcaat accgggactg gcgacaatca
    14221 gatctgccgc catcagccat tcatcattca gactgcccgt gtggcgttct acggcttcgg
    14281 gtaatttatc caggccaggc ggtgtcatac gcgtatccat aacgcgcggc gtcacaccgc
    14341 gagcgaggaa aaagtccacg caggaaagcc cagtgaggcc caggccgata atgacgacat
    14401 ttttaccctg ataatcagcc atgattaacg taccttcagc gttgccagac caatcagaac
    14461 cagcatcagc gaaataatcc agaaacgcac aatgacgcgc ggttccggcc agcctttcag
    14521 ttcatagtgg tgatgaatcg gtgccatgcg gaaaatacgt tgtccgcgca gtttaaagga
    14581 gccgacctgc aggatgacag aaagcgtttc taccacgaac acgcccccca taatcaccag
    14641 caggaattcc tgacgtagca gtacggcgat aatgcctaac gcaccaccta acgccagcga
    14701 acctacatcg cccataaaga cctgcgccgg ataggtgtta aaccacagga agcccagtcc
    14761 tgccccgact atcgcggtac agacaataac cagttcccct gcgtgtcgca gatacggtat
    14821 atgcaagtag ctggcaaagt tcatattgcc ggtcgcccac gccaccagcg caaaaccacc
    14881 ggcgacaaat acggtcggca taattgccag gccgtcgaga ccatcggtca ggtttaccgc
    14941 gttgccagta cccacaatga cgaagtaagc cagcagaatg tagaacagcc ccagctgcgg
    15001 catcacatct ttaaagaatg ggaccaccag ctgcgttgcg ggcgtgtctt tgccggcaag
    15061 gtacagggcg aaggcgacac ccagcgcaat gaccgacatc cagaaatact tccaacgagc
    15121 gatcaaccct ttggtgtctt tacgcaccac tttgcgataa tcatcaacaa agccaataac
    15181 accgtaacct accagcacca ccaacacgca ccagacgtac ggattggacg ggtaagccca
    15241 cagcagtacg gagatcacaa tcgccgtcag gatcataatc ccgcccatgg tcggcgtacc
    15301 gcgcttgctg aagtgtgatt caggaccgtc gttacgcacc acctgaccaa aggaaagttt
    15361 ttgcaaatga gcaatcatac gcgggcccat ccacaatgag atgaacagcg cggtcagcag
    15421 gctgacgatg gcgcgaaacg tcagatagga aaagacgtta aagccggaat aatatttgac
    15481 caaatgttcg gccagccaaa ctaacatgtc ccattctcct gtaaagcgcg tactacctct
    15541 tccatggcgg cactacgtga acccttaact aaaatcgtaa ttaactgttg ctcagcaatc
    15601 agtgatttaa gacgcgtaat taacgcagtt ttatcagcaa aatgttcgcc aacgccgctg
    15661 gcggtgctga tagcatggct ttgtttaccc acgcttaaca cgcggtcaat accagcagct
    15721 tttgccgcct cgcccacctg tacatggcag gcttcgcttt cagcgcccag ttccgccata
    15781 tcgcccacca ccagcacgcg gtagcccggc atttcagcca gtacctgaac agcggcggtc
    15841 attgaaccga cattggcgtt gtaggagtcg tcgagcagca actggttttc tgccagttgg
    15901 atggggaaca gacggcctgg aacagctttc agatttgcca gccccgcttt gatagcatca
    15961 agcgttgcgc ccacggacat ggagagcgca gcggctgcca gcgcattcgc aatattgtga
    16021 cgccccggca acggcagcag aacatcgacg ctaccggttg gggtttgtag ggtaaattcc
    16081 gtaccgtgcg aggtcacatg gatattggtg gcggtgaaat cgctgttggc ggcattaggt
    16141 gagaaacgcc acactttgcg tgagccaatt acgctctgcc agttcagcca gtcgttgttg
    16201 tcggcgttca taatggcgat accgttttcc ggcaggccgc taaagatttc acctttcgct
    16261 ttcgcgacac ccgcaagcga gccaaaacct tccagatgcg ccgctgccag gttgttgacc
    16321 agcgcagctt ccgggcgagt cagactcaca gtccaggcta tttcgccctg atggttcgcg
    16381 ccaagttcaa taactgcgta atcgtattcc ggcgttaagc gcaacaacgt catcggtaca
    16441 ccgatgtcgt tgttgagatt gcctgccgta taaagcgtgt tgccgcactg gcttaaaatc
    16501 gccgccgtca tctctttaac ggaggttttg ccggaggacc ccgtcagagc aaccacgcgc
    16561 gccggaactt gctggcgaac ccatgcagcc agttcaccaa acgccagacg cgtatccttg
    16621 acgattaact gcggcaggtc gatgtccagc ggacggctaa ccagtagtgc gcctgcgccg
    16681 ccagctttcg cctggtcggc aaaatcgtgg gcatcaaaac gttcgccttt cagggcaaca
    16741 aacaggcagc ccggcgtcag ttttcgggta tcagtggtta cagcatcaag ggtgatatct
    16801 gcaccttgca gttcaccgtt gagaatgtcg gtaagttggc taagggttac gctaatcatg
    16861 caatcacccc cagcagacgc gccaccgtga cgcgatcgga gtagtccaga cgctgattgc
    16921 caacaatctg gtaatcttca tggcctttgc ccgcgaccag taccacatca ttctctttag
    16981 cctgcataac ggcgcaagtc accgcttcag cacggccttc catcactttg gcatgtccgg
    17041 catctaacat tcccgccaga atatcgttga tgatggcacg cggttcttcg gtacgcgggt
    17101 tatcgtccgt caccaccgcc acgtcagcaa actcttcggc aattgcgccc atcagtggac
    17161 gcttaccttt atcgcgatcg ccaccacagc caaagacgca ccacagcttg cccgcacagt
    17221 gcaggcgcgc cgcctgtaag gctttttcca gtgcatccgg cgtatgcgcg taatccacca
    17281 ccaccgtcgg tttgcctggc gcagtgaaca cttccatacg tccgcaaacc ggttgcagac
    17341 gcgcggcggt tttcagcaga tcagccagtg gatagccgag tgccagcagt gtcgccagcg
    17401 cgagcagcag gttgctgacg ttaaaagcgc ccatcagatg gctttcaatt tcgccatcgc
    17461 cccaacttga gctaaagcga atcgtcgcac cgctgtcgtg atagttcacg tcgatcgctt
    17521 ttaaccagcg tccgtgacag ttcggattga tatgatcttc cattgatacc gcaaccgcgt
    17581 ccggcagttt tgccagccag cggcggccca cttcatcgtc ggcgttaata atcgcctgac
    17641 cgcaatgatg ttcagaataa agcagccatt tcgcagcttc gtagtgttcc atatcaccat
    17701 gataatcaag gtgatcgcgg cttaagttgg taaagaccga cgccgcaaat ttcaatgccg
    17761 ccacacggtg ctgtaccagc ccgtgggagg aaacttccat tgcgcaaaac gtcgcgccct
    17821 gctccaccag ccccgccagc tcatgctgaa catcgactgc cgaaccggta gtattttctg
    17881 tcgggatcac tttcccaagc agaccgttac caacggtgcc cattaccgcg ctggtttcgc
    17941 caagcagttg gctccactgc gccagcagct gggtagtcgt ggttttgccg ttggtgcccg
    18001 ttacgcctac gagacgtaaa ttgtcagagg gttcatggta aaagcggccc gccagtgcag
    18061 ataaacgctc gttgagctgg ctgagataga tgaccggtac gccgtgcatt tcacggattt
    18121 caccgtcggt cgcctcatct ttcgcctctg caataatggc agccacacct tgcgctatcg
    18181 cctgcgggat atatcgacgc ccgtccgcct gatgacctac tacagctaca aagagatcgc
    18241 ccgccgcagc cacacggctg tcgagtgtca tctctcgcag tgctcgcgaa ggtgcgtctg
    18301 gcacccacgg agcaagaagg tcgcgcaaat tacgatctgc cacctgtccc ctcgccttga
    18361 ttaatcacaa attcattttt atcgcccgtt gtcagcgcat ccggctcgat gttcatggta
    18421 cgcaatacgc cgcccatgat ggcaccaaag accggcgcgg aaacggcgcc gccgtagtat
    18481 ttacccgcct gcggatcgtt gataacaaca accagcgcga agcgcggctg actcgcaggc
    18541 gcaacgcctg cggtataagc aatatattta ttgatgtagc gaccgtccgg cccgaccttt
    18601 ttcgcggtac cggttttaat ggcgatacga tagcctttaa tcgccgcttt cacgccgccg
    18661 ccgcctggta gcgccacgct ttccatcata tgcaccacgg tgcgaacaat ggattccggg
    18721 aagacacgtt caccgggaac cggggggtca actttggtaa tcgacagtgg gcgataaatg
    18781 ccgtagctgc cgatagttgc gtagactcgc gctaactgta acggtgttac cattagcccg
    18841 tagccgaaag agaaggtggc cctctctatg tcagaccacc gttgtttttg aggatataag
    18901 ccactgcgtt ctccgaccaa ccccaaattg gtcgcttttc ccagtccaaa acgtgagtaa
    18961 gtatctacta acgctgagga cggcatcgct aacgccagct tggaaacacc gacgttactc
    19021 gacttctgta ataccccggt cagggttaat tcgctgtagc gtgccacgtc tttgatttcg
    19081 tggccgttaa ttcgataagg aatggtattg agtaccgagt tttcccgcac cacgccacgt
    19141 tgcaacgcgg tcattaccac catcggttta accgttgagc ccggttcaaa cacgtcggtg
    19201 atggtacggt tacgcatcgc ctctttcggc gtgccgctca gattgttagg gttgtatgac
    19261 gggctgttag ccatcgccag cacttcaccg gtgttgacat ccaccagcac ggcgctaccg
    19321 gattcagcct tgttaaaggc caccgcgttg ttcagttcgc gataaaccag cgcctgcaag
    19381 cgttcatcaa tactcaacgc caggttgtgc gccgcctggc tgtcagtaga agaaatatct
    19441 tcaattacgc gaccatagcg gtctttacgc acaatgcgct cacccggctg cccggtaagc
    19501 catttatcga aactcttctc aacgccctca atcccttgac tatcgacgtt agtaaagcca
    19561 atgaggtgag cagtcacttc gccggatgga taataacggc gagattcttc acgcagatga
    19621 atccccggca gtttcagttt tttgatgtag tccgccatgt cagggtttac ctgacgcgcc
    19681 agataaataa agcgcccttt cgggttggcg ttaatgcggg ctgaaagctg atccagcgga
    19741 atattgagcg cgttagccag cgccttccag cggtcaccga cgctgatacc gccagcgtca
    19801 tgcacttctt tcgggtcagc ccaaatcgct tttaccggca cgctcaccgc taacgggcga
    19861 ccagaacggt cagtaatcat gccgcgggag gtggaaactt gctgaacgcg aagagaacgc
    19921 atgtcgccct ctttcaccag catatccggg gagataactt gtaaccacgc tacgcgtccg
    19981 agcagaaaag ccagcgccag gagaatacag ccgcataaca acgcaaaacg ccaactgata
    20041 aagttggcat gttcttcctg acgttttggt ttctgcgttt tcgccgctgc tttcatgcgt
    20101 cgcgtttatc cttatttttg cactacgata ttttcttgtg acggatcaac atgctgcatt
    20161 tgcagttttt ccgtggcgat cctttccacc cggctatggt cgccgagcgc attctcttca
    20221 aggatcaggt tgcgccattc aatgtctaaa gcatctcgct ccagcaccag ttgttcgcgc
    20281 tgagcggtca gtaaacgggt atggtgcgcc gtggttacca cggtcaccgc cgtcaaaata
    20341 atgcaaatga acaggcagag tggcagcttc ccaaatcgca aaagatcgtc accgataaca
    20401 ccaggcaatg catggcgctc gtggcttccc atcgatcctt taactttgct tagagcttct
    20461 gtcactctgc tgatcatgcg ttcgtcctct ctgcaatacg cagaactgaa ctacgggcac
    20521 gaggattctc agctacctct tcttcgcccg gcattaactt gcctaatgct cgcagctgac
    20581 ggccacccag ttttttgagc tgctcttcag tcatcggtaa ccctgccgga acttgcggac
    20641 cgcggctgtt ttcacgcata aaacgtttca caatacggtc ttccagcgag tggaagctga
    20701 tgatcgaaag ccgcccaccc ggggccagca cgttgagcga gctttttagc gcctgctcta
    20761 tctcctccag ttcactgttt acccaaatgc gcaccgcctg gaaggtacgg gtcgcgggat
    20821 gtttgaattt gtctttcacc ggcgttgcgg cagccacgac ttccgccagt tctttggtgc
    20881 gggtcatcgg ctgttcgcgg ttacgctcga caatggcgcg ggcaatgcgt ttggcaaaac
    20941 gctcttcacc ataggttttc aatacccagg cgatatcggc ttcttctgcg gtttgcagcc
    21001 attcagcggc tgactgccca cgggttgggt ccatacgcat gtccagcgga ccatcgcgca
    21061 taaaggaaaa gccgcgctca gcatcatcaa gttgcggtga agagacgcca agatcgagga
    21121 gaatgccgtc gatcttgccg ataagatcgc gctcggcaac gtattcgccc agcgcggaga
    21181 aaggtccgtg gatgatggag aagcgcggat catcaatagt cttcgcaacg gcgatagcct
    21241 gcgggtcgcg atcgatcgcc agcaaacgcc cctcttcgcc aagctgcgag aggatcagac
    21301 gtgagtgacc accgcgacca aaagtcccat caatgtagat gccatcagga cggatattga
    21361 ggccattaac ggcttcatcc agcagcaccg tagtatgttt atagttttcc atcattttat
    21421 agagacaagt cctgcagtcg ctccgataag tctccggtag ccaactgctc tgcgtcgata
    21481 tcttccttga cctgttgatg ccaggttgtt tcatcccaca gctcaaactt gttgaactgt
    21541 ccaaccagca tcacttcttt tgtcagcccg gcatgttgcc gcagtactgg cgcgattaac
    21601 aatcgacctg cgccatccat ctgacattcg ctggcatgac ctaacagtag gcgctgcaca
    21661 cggcgctcaa ccgggttcat gctcgacaga cgcgataatt tttgctcgat aatttcccat
    21721 tcaggcaggg ggtaaagcag caggcacggg tgatgaatgt caatggtgca aaccatttga
    21781 ccggcagcgt tctcaagcag ctgttcccgg taacgggtag gcactgataa gcgccctttg
    21841 ctgtcgagat tgactaacgt tgctccccgg aacatgccag cctcacccct tattcccact
    21901 ttgccccaca atttcccact gaaaggagtt tacggagcgg aggaaaagct tgtcaagcca
    21961 gtcacaaggc ataccgaggt aaaaaacccg atgtttacgg ctaatatcag cgttgtttat
    22021 tttacgtcca acgtgcgcag caaattaacg ttatgaatag ctgtaagaaa aaatccctca
    22081 tcgtcatcac cgttttaaaa tgactcacta ttttccacag caaatataaa gtgtcagttt
    22141 gcgacgcgag cggcatttta ggacatatct tccccggtta acagtccctg ttgcgtctgt
    22201 gtggcgcttg atgccagcgg gtttgcgcaa aagcaaagaa aagtgcttgt taaatcggca
    22261 attcatctct gcatgtaaca aaataatgca aaaacgcgct gtcgatatca cttaattatt
    22321 gtgaatttca gctggtgagt taattaacgg gcgggtttaa aagaacttat caataatagt
    22381 caggaatagt cttatttact ttaaggatat tgatgtccat tcccgtactc tacgcgccag
    22441 agggaaattc acctggcgcg tattattgtt cgcggcttag ctacggctga gcacaccgcg
    22501 gcgatagaga ttacgtttaa tgcgcgttaa accaggtttt ggcttacgcg gttcgtccag
    22561 gcttgccagg acaatctcca gcacacgctc tgcgacatcg cggtgacgtt gagccactgc
    22621 cagcaccggg cactgtaaga agtcgagcag ttcgttatcg ccaaaggtgg caattgcaag
    22681 gtcagaaggc agtttaccat cgcgacgtag cgtgacatcc atcacccctt gcaacaacgc
    22741 aaacgacgtt gtgaacagcg cctgcggcat cggatgcgtt tccagccatt tttcgaataa
    22801 ctgggcagcc gcctcccgct catagctgtt ggcatacagg aaatgcactt cgcgcggatc
    22861 atctttccag gcagtacgga aaccttgttc acgcaggaag ctgacagaaa gctccggcag
    22921 cgcgccaaga taaagcaccg tctcggcggg aaacttacgt aactcttccg ccagcatttc
    22981 ggcatcatcc tgatcggcac caaccacgct ggtgaaatgt tcacgatcga gggcgcggtc
    23041 cagcgcgaca atcgggaacg ggtcgttagc ccagcgttga taaaaaggat gctcaggagg
    23101 caacgatgtc gaaacaataa tggcatcgac ctgacgctgt aaaaggtgct caatacagcg
    23161 catttcgttg tctggctgat cttctgagca ggcaattagc agttgataac cccgttgccg
    23221 cgcctggcgt tcaagatagt tagcgatgcg ggtatagctg gtgttctcca gatcggggat
    23281 cacaagacca atagaacgtg tgcgtccagc acgaagccca gctgccacgg cgttcgggtg
    23341 gtaattgtgc tcacgcacca cagccatgac tttttcaacg gttttgtcgc tcacacggta
    23401 ttgcttcgct ttgccgttaa taacatagct tgcagtggtc cgcgacactc ccgccagccg
    23461 agcgatttca tccagtttca caattgcccc ttgcgtaaaa tgtaaaaacc atagccattg
    23521 tactggcatg ggttaaattc attaacatct aagcgcagaa aattaactgc ggcaactgct
    23581 tttattccag gttcccactg atttcgcaaa aaaagcccaa cgtgacatgt tgggctgcaa
    23641 attgcgcatt cagatcattc tcaacgcatt attttatcgc cgcgcgaaag tccgaccaca
    23701 ccagaacgag caacttccac aattttcgcc acctcgcgaa tcgatgctaa aaatgcatca
    23761 agcttatcgc tggtgcctgc taattgaacg gtataaagcg agggtgtgac atcgataatt
    23821 tgcccacgga atatttccgt attacgtttc acttcgtcac gcccgtaacc gctggcctga
    23881 attttcacca gcatgatttc ccgctcaaca tgcgcgccct gccccaactc gctcacgcgc
    23941 aagacatcga ccagcttgtg taattgcttt tcgatctgct caagcacttt ttcatcgccc
    24001 acggtctgga tggtcatacg cgataatgtc ggatcgtcgg ttggcgcaac ggtcaggctt
    24061 tcaatgttgt agccacgctg ggaaaaaagg ccaatcacgc gggataacgc gcctgattca
    24121 ttttcgagta agactgataa tatccggcgc ataatcaggt tctctccgtt ttgcttaacc
    24181 acatttcatc cattccgccc ccgcgaatct gcatcgggta gacgtgctca ctgccatcga
    24241 cggtaacatc aacaaacacc aggcgattat tgcgcacctg ttccagcgcc tcgctaagtt
    24301 tgctttccag ctcatgcgga tgagagatct ggatcccgac gtgaccatag gcttccgcca
    24361 gacggacgaa atcgggtagt gattgcatat aagattgtga atgacggccg gaatagatca
    24421 tgtcctgcca ctgtttcacc atccccagat agcggttatt gagattcacc accagtacgg
    24481 gcaactcgta ttgcaacgcg gtagacagtt cctggatgtt catctgaata ctgccgtcgc
    24541 cagtgacgca aaccacggtt tcttctggca acgccatttt gacgcccagt gccgcaggta
    24601 aaccaaaacc catcgtgccg aggccaccgg aattgatcca gcgacgcggt ttgtcgaatg
    24661 gataataaag tgcagcaaac atctggtgct gcccgacatc ggacgtcacg taagcgtccc
    24721 cattagtcaa ccgccaaaga gtctcgatca ccgcctgcgg tttaatcttt tcactgtgag
    24781 tgtcatattt caggcactga cgagcgcgcc actgttcaat ttgctgccac cagtcgcgga
    24841 tctcatccag tggttgatgg gcggattctt gcgacaggag ttcaagcatt tgttcgagga
    24901 cctggcgagc atcccccaca atcgggatat ccgcagtcac ggttttagaa atggaagtag
    24961 gatcaatatc gatatgcaga acagtggcat ttgggcagta ctttgccaga ttgttcgtcg
    25021 ttcggtcatc aaatcgtacc ccgacggcga aaatcacatc cgcgttatgc atcgtcatat
    25081 tggcttcgta ggtaccgtgc attcccagca tgcccagtgc ctgacgatgc gttgccggaa
    25141 acgcccccag ccccatcaat gaggaaacaa cgggcagatt caacgcctcc accgtttctt
    25201 tcaactgctg atggcagccc gccgtgattg ccccaccgcc tacgtagaca accggttttt
    25261 ttgccgctac cagcgtttgc agagcaccct taatttgccc tttatgtccg gtagtagtgg
    25321 gattgtaaga acgcatactg accgactcag gccagacata gggtaatttg ttcgccggat
    25381 taagaatatc tttcggtaaa tcaacgacca cgggtcctgg gcgaccactt gccgccagcc
    25441 agaaagcctt tttcagcacc tgcggaatgt cttccgtttg cttaaccaga aaactgtgtt
    25501 taaccaccgg tcgcgaaatc cccaccatgt cgcactcctg aaaggcatcg taacctatca
    25561 acgaggtcgc tacctgcccg gaaaggacaa ctaatggaat ggaatccata taagcggtgg
    25621 cgatgccggt aatcgcattg gtcgcccctg gacccgacgt taccagcacg acgccgactt
    25681 ccccggtcgc acgcgccagg ccatcggcca tatgcaccgc cgcctgctca tgacgaacta
    25741 atacatgatc aataccaccc acggtatgca atgcatcgta aatatcaagg actgcgcctc
    25801 cgggataacc gaatacttgt ttaacgccct gatcgataag cgatcggacg accatctcgg
    25861 ctccagacaa catctccatg gcctgcctca ctgtttgacg gaaaaaatgt gtaaaacaca
    25921 ttaaccgtcc gatcttgctt aagcaattgg cagccagaga cataaacgca gggtaataag
    25981 aataaacagg aggaaaggtg aaaagaaacg ataaaatcct ccattacgaa ggattaaatt
    26041 gataataaat cggctgaatc ccacaactta cagcaatttc tctat
//
LOCUS       DAZFQD010000065        26060 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.065, whole genome shotgun sequence.
ACCESSION   DAZFQD010000065 DAZFQD010000000
VERSION     DAZFQD010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26060
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.065"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            201..395
                     /gene="bfd"
                     /locus_tag="ACHKTW_002710"
     CDS             201..395
                     /gene="bfd"
                     /locus_tag="ACHKTW_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HFO6463708.1"
                     /translation="MYVCLCNGVSDKKIRQAVRQFHPQSFQQLRKFIPVGNQCGKCIR
                     AAREVMQDELMQMPEFKEIA"
     gene            468..944
                     /gene="bfr"
                     /locus_tag="ACHKTW_002711"
     CDS             468..944
                     /gene="bfr"
                     /locus_tag="ACHKTW_002711"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HFO6463709.1"
                     /translation="MKGDVKIINYLNKLLGNELVAINQYFLHARMFKNWGLMRLNDVE
                     YHESIDEMKHADKYIERILFLEGIPNLQDLGKLGIGEDVEEMLQSDLRLELEGAKDLR
                     EAIAYADSVHDYVSRDMMIEILADEEGHIDWLETELDLIGKIGLQNYLQSQIKVKD"
     gene            complement(941..1408)
                     /locus_tag="ACHKTW_002712"
     CDS             complement(941..1408)
                     /locus_tag="ACHKTW_002712"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HFO6463710.1"
                     /translation="MDANLPFLILYACLSVLLFLWDAKHGLLPDRFTCPLLWSGLLFS
                     QVCNPDGLADALWGAIIGYGTFAVIYWGYRILRHKEGLGYGDVKFLAALGAWHTWTFL
                     PRLVFLAASFACGAVVVGLLMRGKESLKNPLPFGPFLAAAGFVVGWDSLLAGR"
     gene            1787..2098
                     /gene="rpsJ"
                     /locus_tag="ACHKTW_002713"
     CDS             1787..2098
                     /gene="rpsJ"
                     /locus_tag="ACHKTW_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFO6463711.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            2131..2760
                     /gene="rplC"
                     /locus_tag="ACHKTW_002714"
     CDS             2131..2760
                     /gene="rplC"
                     /locus_tag="ACHKTW_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFO6463712.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            2771..3376
                     /gene="rplD"
                     /locus_tag="ACHKTW_002715"
     CDS             2771..3376
                     /gene="rplD"
                     /locus_tag="ACHKTW_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFO6463713.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLHKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            3373..3675
                     /gene="rplW"
                     /locus_tag="ACHKTW_002716"
     CDS             3373..3675
                     /gene="rplW"
                     /locus_tag="ACHKTW_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFO6463714.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            3693..4514
                     /gene="rplB"
                     /locus_tag="ACHKTW_002717"
     CDS             3693..4514
                     /gene="rplB"
                     /locus_tag="ACHKTW_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFO6463715.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            4531..4809
                     /gene="rpsS"
                     /locus_tag="ACHKTW_002718"
     CDS             4531..4809
                     /gene="rpsS"
                     /locus_tag="ACHKTW_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFO6463716.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            4824..5156
                     /gene="rplV"
                     /locus_tag="ACHKTW_002719"
     CDS             4824..5156
                     /gene="rplV"
                     /locus_tag="ACHKTW_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFO6463717.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VLVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            5174..5875
                     /gene="rpsC"
                     /locus_tag="ACHKTW_002720"
     CDS             5174..5875
                     /gene="rpsC"
                     /locus_tag="ACHKTW_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFO6463718.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            5888..6298
                     /gene="rplP"
                     /locus_tag="ACHKTW_002721"
     CDS             5888..6298
                     /gene="rplP"
                     /locus_tag="ACHKTW_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFO6463719.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            6298..6489
                     /gene="rpmC"
                     /locus_tag="ACHKTW_002722"
     CDS             6298..6489
                     /gene="rpmC"
                     /locus_tag="ACHKTW_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFO6463720.1"
                     /translation="MKAKELREKSVEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            6489..6743
                     /gene="rpsQ"
                     /locus_tag="ACHKTW_002723"
     CDS             6489..6743
                     /gene="rpsQ"
                     /locus_tag="ACHKTW_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFO6463721.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            6908..7279
                     /gene="rplN"
                     /locus_tag="ACHKTW_002724"
     CDS             6908..7279
                     /gene="rplN"
                     /locus_tag="ACHKTW_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFO6463722.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            7290..7604
                     /gene="rplX"
                     /locus_tag="ACHKTW_002725"
     CDS             7290..7604
                     /gene="rplX"
                     /locus_tag="ACHKTW_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFO6463723.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            7619..8158
                     /gene="rplE"
                     /locus_tag="ACHKTW_002726"
     CDS             7619..8158
                     /gene="rplE"
                     /locus_tag="ACHKTW_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFO6463724.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            8173..8478
                     /gene="rpsN"
                     /locus_tag="ACHKTW_002727"
     CDS             8173..8478
                     /gene="rpsN"
                     /locus_tag="ACHKTW_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFO6463725.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            8512..8904
                     /gene="rpsH"
                     /locus_tag="ACHKTW_002728"
     CDS             8512..8904
                     /gene="rpsH"
                     /locus_tag="ACHKTW_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFO6463726.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            8917..9450
                     /gene="rplF"
                     /locus_tag="ACHKTW_002729"
     CDS             8917..9450
                     /gene="rplF"
                     /locus_tag="ACHKTW_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFO6463727.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            9460..9813
                     /gene="rplR"
                     /locus_tag="ACHKTW_002730"
     CDS             9460..9813
                     /gene="rplR"
                     /locus_tag="ACHKTW_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFO6463728.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            9828..10331
                     /gene="rpsE"
                     /locus_tag="ACHKTW_002731"
     CDS             9828..10331
                     /gene="rpsE"
                     /locus_tag="ACHKTW_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFO6463729.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            10335..10514
                     /gene="rpmD"
                     /locus_tag="ACHKTW_002732"
     CDS             10335..10514
                     /gene="rpmD"
                     /locus_tag="ACHKTW_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFO6463730.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            10518..10952
                     /gene="rplO"
                     /locus_tag="ACHKTW_002733"
     CDS             10518..10952
                     /gene="rplO"
                     /locus_tag="ACHKTW_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFO6463731.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            10960..12291
                     /gene="secY"
                     /locus_tag="ACHKTW_002734"
     CDS             10960..12291
                     /gene="secY"
                     /locus_tag="ACHKTW_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFO6463732.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            12323..12439
                     /gene="rpmJ"
                     /locus_tag="ACHKTW_002735"
     CDS             12323..12439
                     /gene="rpmJ"
                     /locus_tag="ACHKTW_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFO6463733.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            12586..12942
                     /gene="rpsM"
                     /locus_tag="ACHKTW_002736"
     CDS             12586..12942
                     /gene="rpsM"
                     /locus_tag="ACHKTW_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFO6463734.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            12959..13348
                     /gene="rpsK"
                     /locus_tag="ACHKTW_002737"
     CDS             12959..13348
                     /gene="rpsK"
                     /locus_tag="ACHKTW_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFO6463735.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            13382..14002
                     /gene="rpsD"
                     /locus_tag="ACHKTW_002738"
     CDS             13382..14002
                     /gene="rpsD"
                     /locus_tag="ACHKTW_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFO6463736.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            14028..15017
                     /gene="rpoA"
                     /locus_tag="ACHKTW_002739"
     CDS             14028..15017
                     /gene="rpoA"
                     /locus_tag="ACHKTW_002739"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFO6463737.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            15058..15441
                     /gene="rplQ"
                     /locus_tag="ACHKTW_002740"
     CDS             15058..15441
                     /gene="rplQ"
                     /locus_tag="ACHKTW_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFO6463738.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
     gene            15548..15916
                     /locus_tag="ACHKTW_002741"
     CDS             15548..15916
                     /locus_tag="ACHKTW_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HFO6463739.1"
                     /translation="MWLLDQWAERHIAEAQAKGEFDNLAGSGEPLILDDDSHVPPELR
                     AGYRLLKNAGCLPPELEQRREAIQLLDILKGIRHDDPQYQEVSRRLSLLELKLRQAGL
                     STDFLRGDYADKLLNKINDN"
     gene            15927..16352
                     /gene="zntR"
                     /locus_tag="ACHKTW_002742"
     CDS             15927..16352
                     /gene="zntR"
                     /locus_tag="ACHKTW_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(2+)-responsive transcriptional regulator"
                     /protein_id="HFO6463740.1"
                     /translation="MYRIGELAKMAEVTPDTIRYYEKQQMMEHEVRTEGGFRLYTESD
                     LQRLKFIRHARQLGFSLESIRELLSIRIDPEHHTCQESKGIVQERLQEVEARIAELQS
                     MQRSLQRLNDACCGTAHSSVYCSILEALEQGASGVKSGC"
     gene            16408..16626
                     /gene="arfA"
                     /locus_tag="ACHKTW_002743"
     CDS             16408..16626
                     /gene="arfA"
                     /locus_tag="ACHKTW_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor ArfA"
                     /protein_id="HFO6463741.1"
                     /translation="MSRYQHTKGQIKDNAIEALLHDPLFRQRVEKNKKGKGSYMRKGK
                     HGNRGNWEASGKKVNHFFTTGLLLSGVC"
     gene            complement(16623..17033)
                     /gene="mscL"
                     /locus_tag="ACHKTW_002744"
     CDS             complement(16623..17033)
                     /gene="mscL"
                     /locus_tag="ACHKTW_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HFO6463742.1"
                     /translation="MSIIKEFREFAMRGNVVDLAVGVIIGAAFGKIVSSLVADIIMPP
                     LGLLIGGIDFKQFAVTLRDAQGDIPAVVMHYGVFIQNVFDFLIVAFAIFMAIKLINKL
                     NRKKEEPAAAPAPTKEEVLLTEIRDLLKEQNNRS"
     gene            complement(17163..18539)
                     /gene="trkA"
                     /locus_tag="ACHKTW_002745"
     CDS             complement(17163..18539)
                     /gene="trkA"
                     /locus_tag="ACHKTW_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HFO6463743.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(18561..19850)
                     /gene="rsmB"
                     /locus_tag="ACHKTW_002746"
     CDS             complement(18561..19850)
                     /gene="rsmB"
                     /locus_tag="ACHKTW_002746"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFO6463744.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRQQEELLAEFNASDARYLHPSWLLKRLQKAYPEQ
                     WQSIVEANNQRPPMWLRVNRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRVYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNLPGAEEGDGFFYAKLIKK"
     gene            complement(19896..20843)
                     /gene="fmt"
                     /locus_tag="ACHKTW_002747"
     CDS             complement(19896..20843)
                     /gene="fmt"
                     /locus_tag="ACHKTW_002747"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFO6463745.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPIKVLAEEKGLPVFQPVSLRPQENQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINVHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLV"
     gene            complement(20858..21367)
                     /gene="def"
                     /locus_tag="ACHKTW_002748"
     CDS             complement(20858..21367)
                     /gene="def"
                     /locus_tag="ACHKTW_002748"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFO6463746.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEADGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            21497..22621
                     /gene="dprA"
                     /locus_tag="ACHKTW_002749"
     CDS             21497..22621
                     /gene="dprA"
                     /locus_tag="ACHKTW_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HFO6463747.1"
                     /translation="MVDTEIWLRLMSISSLYGDDMVRIAHWLAKQSHIDAVVLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLQATTDYPGALFVEGEL
                     HALHSFQLAIVGSRAHSWYGERWGRLFCETLATRGVTITSGLARGIDGVAHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLAASLLEHGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            22593..23066
                     /gene="smg"
                     /locus_tag="ACHKTW_002750"
     CDS             22593..23066
                     /gene="smg"
                     /locus_tag="ACHKTW_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 family protein Smg"
                     /protein_id="HFO6463748.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFDREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDNAEFELDDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            23095..23637
                     /locus_tag="ACHKTW_002751"
     CDS             23095..23637
                     /locus_tag="ACHKTW_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HFO6463749.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCPACGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            23642..24214
                     /gene="tsaC"
                     /locus_tag="ACHKTW_002752"
     CDS             23642..24214
                     /gene="tsaC"
                     /locus_tag="ACHKTW_002752"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HFO6463750.1"
                     /translation="MNNNLQGDAIAAAIDVLNEERVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDAQRETIFSRWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            24219..25037
                     /gene="aroE"
                     /locus_tag="ACHKTW_002753"
     CDS             24219..25037
                     /gene="aroE"
                     /locus_tag="ACHKTW_002753"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFO6463751.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFSAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLQGDNTD
                     GIGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKQLQEELSA"
     gene            25034..25291
                     /locus_tag="ACHKTW_002754"
     CDS             25034..25291
                     /locus_tag="ACHKTW_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HFO6463752.1"
                     /translation="MNQAIQFPDREEWDENKKCVCFPALVNGMQLTCAISGESLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(25267..25821)
                     /locus_tag="ACHKTW_002755"
     CDS             complement(25267..25821)
                     /locus_tag="ACHKTW_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFO6463753.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
BASE COUNT         6697 a         6184 c         6751 g         6428 t
ORIGIN      
        1 gctaaagttc tgggctaatt acacgttaat tagttttgaa ttgaaaaggg cgcttcggcg
       61 ccctttttgc tgcccgtaat ttgagctcga aatatttagt cgtaattttg ctcgctgaac
      121 tatggcgcaa ataaaactgt gctattgtaa tcataaccat tctcatttac actttgcgca
      181 gaatttgaac gggagcaatc atgtacgttt gtttgtgtaa tggtgtaagt gacaaaaaaa
      241 ttcgccaggc agtccgtcag tttcatccgc aatctttcca acaattgcgt aagtttattc
      301 ctgtgggaaa tcaatgcggt aagtgcattc gcgccgcgcg cgaagtgatg caggacgagt
      361 tgatgcagat gccagaattt aaagagattg cctgaagacc acgcttattt tttgacatcc
      421 cgctagcccc atctacgctc taatagtgga agcggaggga ctataaaatg aaaggtgatg
      481 ttaaaatcat aaattatctc aataaactat tgggaaatga gcttgtcgca atcaatcagt
      541 attttctcca tgcccggatg tttaaaaact ggggcctgat gcgtctcaat gacgtcgaat
      601 accatgaatc catcgatgag atgaaacacg ctgataaata tatcgagcgt attttgtttt
      661 tagaaggaat ccccaactta caggatttag gcaaactggg gattggtgaa gatgtcgagg
      721 agatgctgca atccgacctg cgattagaac tggaaggcgc gaaggatctc cgtgaggcta
      781 tcgcgtatgc cgacagcgtt catgactatg ttagccgtga tatgatgatc gagatccttg
      841 ccgatgaaga aggtcatatt gactggctcg aaactgagct ggatctcatc ggtaaaatcg
      901 gtttacaaaa ttacctgcaa tcacaaataa aagttaaaga ttaacggcct gctagcaaac
      961 tatcccaacc tacaacgaaa cccgcagccg ccagaaatgg cccaaacggc agcgggtttt
     1021 ttaatgactc ttttcctctc atgagcagac caacaactac ggctccgcaa gcgaatgagg
     1081 cagcaaggaa aaccagtcgt ggcaagaagg tccaggtatg ccaggcacca agggcggcga
     1141 gaaacttcac atcaccatag cctaatcctt ctttatgacg cagtatgcga tacccccagt
     1201 aaatgacagc gaatgtgccg taaccaataa tcgcccccca taacgcatcg gctaaaccgt
     1261 caggattaca aacctgagag aacaaaagac ctgaccagag taacgggcag gtaaatctgt
     1321 caggtaacaa gccatgcttt gcatcccata aaaaaagtag gacagataaa caagcgtaca
     1381 aaatcagaaa gggaaggtta gcgtccataa aacatcctct gtaattggag tatcaccatc
     1441 gaataactcc ggatgctcag caacagagaa atcgctgatt tgttaacctg gttccggtga
     1501 aaaacgctat cttcacaacc ttacaaattt cgtggaatac agctcgcaat ctgcgcgttg
     1561 cgattaattt ctacccgatg cttgcgtcag gctcagattt gcgtataatg cgcgggcttg
     1621 tcgtagttga cagcaggttc aatctgaacc tcagttagcc gaatttggct acctaacaat
     1681 gctcccaatc ggggagctac gtaagaacgg ttacactctc ccatcaatcg taatgggtct
     1741 gaggagtaat cattttcgtt tataaaataa ttggagctct ggtctcatgc agaaccaaag
     1801 aatccgtatc cgcctgaaag cgtttgatca tcgtctgatc gatcaagcaa ccgcggaaat
     1861 cgtcgagact gccaagcgca ctggtgcgca ggtccgtggt ccgatcccgc tgccgacacg
     1921 caaagagcgc ttcactgttc tgatctcccc gcacgtcaac aaagacgcgc gcgatcagta
     1981 cgaaatccgt actcacttgc gtctggttga catcgttgag ccaaccgaga aaaccgttga
     2041 tgctctgatg cgtctggatc tggctgccgg tgtagacgtg cagatcagcc tgggttaatc
     2101 aggtcattga gcgattgaga ggttgaaaca atgattggtt tagtcggtaa aaaagtgggt
     2161 atgacccgta tcttcacaga agacggcgtt tctatcccag taaccgtaat cgaagttgaa
     2221 gcaaaccgcg ttactcaggt taaagacctg gctaacgatg gctaccgtgc tattcaggtg
     2281 accaccggtg ctaaaaaagc taaccgtgtg accaagcctg aagctggcca cttcgctaaa
     2341 gctggcgtag aagctggccg tggtctgtgg gaattccgcc tggctgaagg cgaagagttc
     2401 actgtaggtc agagcattag cgttgaactg tttgctgacg ttaaaaaagt tgacgtaact
     2461 ggcacctcta aaggtaaagg tttcgcaggt accgttaagc gctggaactt ccgtacccag
     2521 gacgctactc acggtaactc cttgtctcac cgcgttccgg gttctatcgg tcagaaccag
     2581 actccgggca aagtgttcaa aggcaagaaa atggcaggtc agatgggtaa cgaacgtgta
     2641 accgttcaga gccttgacgt agtacgcgtt gacgctgagc gcaacctgct gctggttaaa
     2701 ggtgctgtcc cgggtgcaac cggtagcgac ctgatcgtta aaccagctgt gaaggcgtaa
     2761 ggagatagca atggaattag tattgaaaga cgcgcagagc gcgctgactg tttccgaaac
     2821 taccttcggt cgtgatttca acgaagcgct ggttcaccag gttgttgttg cttatgcagc
     2881 tggtgctcgt cagggtactc gtgctcagaa gactcgtgct gaagtaactg gttccggtaa
     2941 aaaaccgtgg cgccagaaag gcaccggccg tgcgcgttct ggttctatca agagcccgat
     3001 ctggcgttct ggtggcgtga cctttgctgc tcgtccgcag gaccacagtc aaaaagttaa
     3061 caagaagatg taccgcggcg cgctgaaaag catcctgtcc gaactggtac gtcaggatcg
     3121 tctgatcgtt gtcgagaagt tctctgtaga agcgccgaaa actaagctgc tggcacagaa
     3181 actgaaagac atggctctgg aagatgtgct gatcatcacc ggtgagctgg acgaaaacct
     3241 gttcctggct gcgcgcaacc tgcacaaggt tgacgtacgc gatgcaactg gtatcgaccc
     3301 ggttagcctg atcgccttcg acaaagtcgt aatgactgct gatgctgtta agcaagttga
     3361 ggagatgctg gcatgattcg tgaagaacgt ctgctgaagg tgctgcgtgc accgcacgtt
     3421 tctgaaaaag cgtctactgc gatggaaaaa tccaacacca tcgtactcaa agttgctaaa
     3481 gacgcgacca aagcagaaat caaagctgct gtgcagaaac tgtttgaagt cgaagtcgaa
     3541 gtcgttaaca ccctggtagt taaagggaaa gttaaacgtc acggacagcg tatcggtcgt
     3601 cgtagcgact ggaaaaaagc ttacgtcacc ctgaaagaag gccagaatct ggacttcgtt
     3661 ggcggcgctg agtaagtcgg aggagtaata caatggcagt tgttaaatgt aaaccgacat
     3721 ctccgggtcg tcgccacgta gttaaagtgg ttaaccctga gctgcacaag ggcaaacctt
     3781 ttgctccgtt gctggaaaaa aacagcaaat ccggtggtcg taacaacaat ggccgtatca
     3841 ccactcgtca tatcggtggt ggccacaagc aggcttaccg tattgttgac ttcaaacgca
     3901 acaaagacgg tatcccggca gttgttgaac gtcttgagta cgatccgaac cgttccgcga
     3961 acatcgcgct ggttctgtac aaagacggtg aacgccgtta catcctggcc cctaaaggcc
     4021 tgaaagctgg cgaccagatt cagtctggcg ttgatgctgc aatcaaacca ggtaacaccc
     4081 tgccgatgcg caacatcccg gttggttcta ctgttcataa cgtagaaatg aaaccaggta
     4141 aaggcggtca gctggcacgt tccgctggta cttacgttca gatcgttgct cgtgatggtg
     4201 cttatgtcac cctgcgtctg cgttctggtg aaatgcgtaa agtagaagca gactgccgtg
     4261 caactctggg cgaagttggc aatgctgagc atatgctgcg cgttctgggt aaagcaggtg
     4321 ctgcacgctg gcgtggtgtt cgtccgaccg ttcgcggtac cgcgatgaac ccggtagacc
     4381 acccacatgg tggtggtgaa ggtcgtaact ttggtaagca cccggtaact ccgtggggcg
     4441 ttcagaccaa aggtaagaag acccgcagca acaagcgtac tgataaattc atcgtacgtc
     4501 gccgtagcaa ataattttag aggataagcc atgccacgtt ctctcaagaa aggtcctttt
     4561 attgacctgc acttgctgaa gaaggtagag aaagcggtgg aaagcggaga caagaagccc
     4621 ctgcgcactt ggtcccgtcg ttcaacgatc tttcctaaca tgatcggttt gaccatcgct
     4681 gtccataatg gtcgtcagca cgttccggta tttgtaaccg acgaaatggt cggtcacaaa
     4741 ctgggtgaat tcgcaccgac tcgtacttat cgcggccacg ctgctgataa aaaagcgaag
     4801 aagaaataag gtaggaggaa gagatggaaa ctatcgctaa acatcgccat gctcgttctt
     4861 ctgctcagaa ggttcgcctt gttgctgacc tgattcgcgg taagaaagtg tcgcaggctc
     4921 tggatatttt gacctacacc aacaagaaag cggctgtact ggtcaagaaa gttctggaat
     4981 ctgccattgc taacgctgaa cacaacgatg gcgctgacat tgacgatctg aaagttacga
     5041 aaattttcgt agacgaaggc ccgagcatga agcgcattat gccgcgtgca aaaggtcgtg
     5101 cagatcgcat cctgaagcgc accagccaca tcactgtggt tgtgtccgat cgctgagact
     5161 ctggagacta gcaatgggtc agaaagtaca tcctaatggt attcgcctgg gtattgtaaa
     5221 accatggaac tctacctggt ttgcgaacac caaagaattc gctgacaacc tggacagcga
     5281 ttttaaagta cgtcagtacc tgactaagga actggctaaa gcgtccgtat ctcgtatcgt
     5341 tatcgagcgt ccggctaaga gcatccgtgt aaccattcac actgctcgcc cgggtatcgt
     5401 tatcggtaaa aaaggtgaag acgtagaaaa actgcgtaag gtcgtagcgg acatcgctgg
     5461 cgttcctgca cagatcaaca tcgccgaagt tcgtaagcct gaactggacg caaaactggt
     5521 tgctgacagc atcacttctc agctggaacg tcgcgttatg ttccgtcgtg ctatgaagcg
     5581 tgctgtacag aacgcaatgc gtctgggcgc taaaggtatt aaagttgaag ttagcggccg
     5641 tctgggcggc gcggaaatcg cacgtaccga atggtaccgc gaaggtcgcg taccgctgca
     5701 cactctgcgt gctgacatcg actacaacac ctctgaagcg cacaccactt acggtgtaat
     5761 cggcgttaaa gtgtggatct tcaaaggcga gatcctgggt ggtatggctg ctgttgaaca
     5821 accggaaaaa ccggctgctc agcctaaaaa gcagcagcgt aaaggccgta aataaggagc
     5881 gtcgctgatg ttacaaccaa agcgtacaaa attccgtaaa atgcacaaag gccgtaaccg
     5941 cggtctggcg cagggtacgg atgttagctt cggcagcttc ggtctgaaag ctgttggccg
     6001 tggtcgtctg actgcccgtc agatcgaagc agcacgtcgt gctatgaccc gtgcagttaa
     6061 gcgtcaaggt aagatctgga tccgtgtgtt cccggacaaa ccgatcactg aaaagccgct
     6121 ggcagtgcgt atgggtaaag gtaaaggtaa cgtggagtat tgggttgcct tgattcagcc
     6181 gggtaaagtc ctgtatgaaa tggacggtgt tccggaagag ctggcccgtg aagcattcaa
     6241 gctggcagca gcgaaactgc cgattaaaac cacctttgta actaagacgg tgatgtaatg
     6301 aaagcaaaag agctgcgtga gaagagcgtt gaagagctga acaccgagct gctgaacctg
     6361 ctgcgtgagc agttcaacct gcgtatgcag gctgcaagtg gccagctgca acagtctcac
     6421 ctgttgaagc aagtgcgtcg cgatgtcgca cgcgttaaga ctttactgaa cgagaaggcg
     6481 ggtgcgtaat gaccgataaa atccgtactc tgcaaggtcg cgttgttagc gacaaaatgg
     6541 agaaatccat tgttgttgct atcgaacgtt ttgtgaaaca cccgatctac ggtaaattca
     6601 tcaagcgtac gaccaaactg cacgtacatg acgagaacaa cgaatgcggt atcggtgacg
     6661 tggttgaaat ccgcgaatgc cgtccgctgt ccaagactaa atcctggacg ctggttcgcg
     6721 ttgtagagaa agcggttctg taatacagta cactctctcg atacgaataa acggctcaga
     6781 aatgagccgt ttattttttc tacccatatc cttgaagcgg tgttataatg ccgcgccctc
     6841 gatatgggga tttttaacga cctgattttc gggtctcagt agtagttgac attagcggag
     6901 cactaaaatg atccaagaac agactatgct gaacgtcgcc gacaactccg gtgcacgtcg
     6961 cgtaatgtgt atcaaggttc tgggtggctc gcaccgtcgc tacgcaggcg taggcgacat
     7021 catcaagatc accatcaaag aagcaattcc gcgtggtaag gtcaaaaaag gtgatgtgct
     7081 gaaggcggta gtggtgcgca ccaagaaggg tgttcgtcgc ccggacggtt ctgtcattcg
     7141 cttcgatggt aatgcttgtg ttcttctgaa caacaacagc gagcagccta tcggtacgcg
     7201 tatttttggg ccggtaactc gtgagcttcg tagtgagaag ttcatgaaaa ttatctctct
     7261 ggcaccagaa gtactctaag gagcgaatca tggcagcgaa aatccgtcgt gatgacgaag
     7321 ttatcgtgtt aaccggtaaa gataaaggta aacgcggtaa agttaagaat gtcctgtctt
     7381 ccggcaaggt cattgttgaa ggtatcaacc tggttaagaa acatcagaag ccggttccgg
     7441 ccctgaacca accgggtggc atcgttgaaa aagaagccgc tattcaggtt tccaacgtag
     7501 caatcttcaa tgcggcaacc ggcaaggctg accgtgtagg ctttagattc gaagacggca
     7561 aaaaagtccg tttcttcaag tctaacagcg aaactatcaa gtaatttgga gtagtacgat
     7621 ggcgaaactg catgattact acaaagacga agtagttaaa aaactcatga ctgagtttaa
     7681 ctacaattct gtcatgcaag tccctcgggt cgagaagatc accctgaaca tgggtgttgg
     7741 tgaagcgatc gctgacaaaa aactgctgga taacgcagca gcagacctgg cagcaatctc
     7801 cggtcaaaaa ccgctgatca ccaaagcacg caaatctgtt gcaggcttca aaatccgtca
     7861 gggctatccg atcggctgta aagtaactct gcgtggcgaa cgcatgtggg agttctttga
     7921 gcgcctgatc actattgctg tacctcgtat ccgtgacttc cgtggcctgt ccgctaagtc
     7981 tttcgacggt cgtggtaact acagcatggg tgtccgtgag cagatcatct tcccagaaat
     8041 cgactacgat aaagtcgacc gcgttcgtgg tttggatatt accattacca ctactgcgaa
     8101 atctgacgaa gaaggccgcg ctctgctggc tgcctttgac ttcccgttcc gcaagtaagg
     8161 tagggttact aaatggctaa gcaatcaatg aaagcacgcg aagtaaaacg cgtagcttta
     8221 gctgataaat acttcgcgaa acgcgctgaa ctgaaagcga tcatctctga tgtgaacgct
     8281 tccgacgaag atcgttggaa cgctgttctc aagctgcaga ctctgccgcg tgattccagc
     8341 ccgtctcgtc agcgtaaccg ctgccgtcaa acaggtcgtc cgcatggttt cctgcggaag
     8401 ttcgggttga gccgtattaa ggtccgtgaa gccgctatgc gcggtgaaat cccgggtctg
     8461 aaaaaggcta gctggtaatt gtcaccaatt gaatcacggg agtaaagaca gatgagcatg
     8521 caagatccga tcgcggatat gctgacccgt atccgtaacg gtcaggccgc gaacaaagct
     8581 gcggtcacca tgccttcctc caagctgaaa gtggcaatcg ccaacgtgct gaaggaagaa
     8641 ggttttattg aagattttaa agttgaaggc gacaccaagc ctgaactgga acttactctg
     8701 aagtatttcc agggcaaagc tgttgtagaa agcattcagc gtgtcagccg cccaggtctg
     8761 cgcatctata aacgtaaaga tgagctgccg aaagttatgg cgggtctggg tatcgcagtt
     8821 gtttctacct ctaaaggtgt tatgactgat cgtgcagcgc gccaggctgg tcttggtggc
     8881 gaaattatct gctacgtagc ctaatcggag gaaaaaatgt ctcgtgttgc taaagcaccg
     8941 gtcgttgttc ctgccggcgt tgacgtaaaa atcaacggtc aggttattac gatcaaaggt
     9001 aaaaacggcg agctgactcg tactctcaac gatgctgttg aagttaaaca tgcagataat
     9061 accctgacct tcggtccgcg tgatggttac gcagacggtt gggcacaggc tggtaccgcg
     9121 cgtgccctgc tgaactcaat ggttatcggt gttaccgaag gcttcactaa gaagctgcag
     9181 ctggttggtg taggttaccg tgcagcggtt aaaggcaatg tgattaacct gtctctgggt
     9241 ttctctcatc ctgttgacca tcagctgcct gcgggtatca ctgctgaatg tccgactcag
     9301 actgaaatcg tgctgaaagg cgctgataag caggtgatcg gccaggttgc agcggatctg
     9361 cgcgcctacc gtcgtcctga gccttataaa ggcaagggtg ttcgttacgc cgacgaagtc
     9421 gtgcgtacca aagaggctaa gaagaagtaa ggtaacacta tggataagaa atctgctcgt
     9481 atccgtcgtg cgacccgcgc acgccgcaag ctccaggagc tgggcgcaac tcgcctggtg
     9541 gtacatcgta ccccgcgtca catttacgca caggtaattg caccgaacgg ttctgaagtt
     9601 ctggtagctg cttctactgt agaaaaagct atcgctgaac aactgaagta caccggtaac
     9661 aaagacgcgg ctgcagctgt gggtaaagct gtcgctgaac gcgctctgga aaaaggcatc
     9721 aaagatgtat cctttgaccg ttccgggttc caatatcatg gtcgtgtcca ggcactggca
     9781 gatgctgccc gtgaagctgg ccttcagttc taaggtagag gtgtaagatg gctcacatcg
     9841 aaaaacaagc tggcgaactg caggaaaagc tgatcgcggt aaaccgcgta tctaaaaccg
     9901 ttaaaggtgg tcgtattttc tccttcacag ctctgactgt agttggcgat ggtaacggtc
     9961 gcgttggttt tggttacggt aaagcgcgtg aagttccagc agcgatccag aaagcgatgg
    10021 aaaaagcccg tcgcaatatg attaacgtcg cgctgaataa cggcactctg caacaccctg
    10081 ttaaaggtgt tcacacgggt tctcgcgtat tcatgcagcc ggcttccgaa ggtaccggta
    10141 tcatcgccgg tggtgcaatg cgcgccgttc tggaagtcgc tggggttcat aacgttctgg
    10201 ctaaagcata tggttccacc aacccgatca acgtggttcg tgcaactatt gatggcctgg
    10261 aaaatatgaa ttctccagaa atggtcgctg ccaagcgtgg taaatccgtt gaagaaattc
    10321 tggggaaata aaccatggca aagactatta aaattactca aacccgcagt gcaatcggtc
    10381 gtctgccgaa acacaaggca acgctgcttg gcctgggtct gcgtcgtatt ggtcacaccg
    10441 tagagcgcga ggatactcct gctattcgcg gtatgatcaa cgcggtttcc ttcatggtta
    10501 aagttgagga gtaagagatg cgtttaaata ctctgtctcc ggccgaaggc tccaaaaagg
    10561 cgggtaaacg cctgggtcgt ggtatcggtt ctggcctcgg taaaaccggt ggtcgtggtc
    10621 acaaaggtca gaaatctcgt tctggcggtg gcgtacgtcg cggtttcgag ggtggtcaga
    10681 tgcctctgta ccgtcgtctg ccgaaattcg gcttcacttc tcgtaaagca gcgattacag
    10741 ccgaagttcg tctgtctgac ctggctaaag tagaaggcgg tgttgtagac ctgaacacgc
    10801 tgaaagcggc taacattatc ggtatccaga tcgagttcgc gaaagtgatc ctggctggcg
    10861 aagtaacgac tccggtaact gttcgtggcc tgcgtgttac taaaggcgct cgtgctgcta
    10921 tcgaagctgc tggcggtaaa atcgaggaat aagtagcaga tggctaaaca accgggatta
    10981 gattttcaaa gtgccaaagg tggcttaggc gagctgaaac gcagactgct gtttgttatc
    11041 ggtgcgctga ttgtgttccg tattggctct tttattccga tccctggtat tgatgccgct
    11101 gtacttgcca aactgcttga gcaacagcga ggcaccatca ttgagatgtt taacatgttc
    11161 tctggtggtg ctctcagccg tgcttctatc tttgctctgg ggatcatgcc gtatatttcg
    11221 gcgtcgatca ttatccagct gctgacggtg gttcacccaa cgttggcaga aattaagaaa
    11281 gaaggggagt ctggtcgtcg taagatcagc cagtacaccc gttacggtac tctggtgctg
    11341 gcaatattcc agtcgatcgg tattgctacc ggtctgccga atatgcctgg tatgcaaggc
    11401 ctggtgatta acccaggctt tgcattctac ttcaccgctg ttgtaagtct ggtcacagga
    11461 acgatgttcc tgatgtggtt gggcgaacag attactgaac gaggtatcgg caacggtatt
    11521 tcaatcatta tcttcgccgg tattgtcgcg ggactcccgc cagccattgc ccatactatc
    11581 gagcaagcgc gtcaaggcga cctgcacttc ctcgtgttgc tgttggttgc agtattagta
    11641 tttgcagtga cgttctttgt tgtatttgtt gagcgtggtc aacgccgcat tgtggtaaac
    11701 tacgcgaaac gtcagcaagg tcgtcgtgtc tatgctgcac agagcacaca tttaccgctg
    11761 aaagtgaata tggcgggggt aatcccggca atcttcgctt ccagtattat tctgttcccg
    11821 gcgaccatcg cgtcatggtt cgggggcggt actggttgga actggctgac aacaatttcg
    11881 ctgtatttgc agcctgggca accgctttat gtgttactct atgcgtctgc aatcatcttc
    11941 ttctgtttct tctacacggc gttggttttc aacccgcgtg aaacagcaga taacctgaag
    12001 aagtccggtg catttgtacc aggaattcgt ccgggagagc aaacggcgaa gtatatcgat
    12061 aaagtaatga cccgcctgac cctggttggt gcgctgtata ttacctttat ctgcctgatc
    12121 ccggagttca tgcgtgatgc aatgaaagta ccgttctact tcggtgggac ctcactgctt
    12181 atcgttgttg tcgtgattat ggactttatg gctcaagtgc aaactctgat gatgtccagt
    12241 cagtatgagt ctgcattgaa gaaggcgaac ctgaaaggct acggccgata attggtcgcc
    12301 cgagaagtta cggagagtaa aaatgaaagt tcgtgcttcc gtcaagaaat tatgccgtaa
    12361 ctgcaaaatc gttaagcgtg atggtgtcat ccgtgtgatt tgcagtgccg agccgaagca
    12421 taaacagcgc caaggctgat tttttcgcat atttttcttg caaagttggg ttgagctggc
    12481 tagattagcc agccaatctt ttgtatgtct gtgcgtttcc atttgagtat cctgaaaacg
    12541 ggcttttcag catggaacgt acatattaaa tagtaggagt gcatagtggc ccgtatagca
    12601 ggcattaaca ttcctgatca taagcatgcc gtaatcgcat taacttcgat ttatggcgtc
    12661 ggcaagaccc gttctaaagc catcctggct gcagcgggta tcgctgaaga tgttaagatc
    12721 agtgagctgt ctgaaggaca aatcgacacg ctgcgtgacg aagttgccaa atttgtcgtt
    12781 gaaggtgatc tgcgccgtga aatcagcatg agcatcaagc gcctgatgga tcttggttgc
    12841 tatcgcggtt tgcgtcatcg tcgtggtctc ccggttcgcg gtcagcgtac caagaccaac
    12901 gcacgtaccc gtaagggtcc gcgcaaaccg atcaagaaat aatcggggtg attgaataat
    12961 ggcaaaggca ccaattcgtg cacgtaaacg tgtaagaaaa caagtctctg acggcgtggc
    13021 tcatatccat gcttctttca acaacaccat cgtgactatc actgatcgtc agggtaacgc
    13081 gttgggttgg gcaacagccg gtggttccgg tttccgtggt tctcgcaaat ccactccgtt
    13141 tgcagctcag gttgcagcag agcgttgcgc tgacgccgtg aaagaatacg gcatcaagaa
    13201 tctggaagtt atggttaaag gtccgggtcc aggccgcgaa tctactattc gtgctctgaa
    13261 cgccgcaggt ttccgcatca ctaatattac tgatgtgact ccgatccctc ataacggttg
    13321 tcgtccgccg aaaaaacgtc gcgtataacg cctcgttttc caggtttgtt ggagaaagaa
    13381 aatggcaaga tatttgggtc ctaagctcaa gctgagccgt cgtgagggca ccgacttatt
    13441 ccttaagtct ggcgttcgcg cgatcgatac caagtgtaaa attgaacaag ctcctggcca
    13501 gcacggtgcg cgtaaaccgc gtctgtctga ctatggtgtg cagttgcgtg aaaagcaaaa
    13561 agttcgccgt atctatggtg tgctggagcg tcagttccgt aactactaca aagaagcagc
    13621 acgtctgaaa ggcaacaccg gtgaaaacct gttggctctg ctggaaggtc gtctggacaa
    13681 cgttgtatac cgtatgggct tcggtgccac tcgtgcagaa gcacgtcagc tggttagcca
    13741 taaagcaatt atggtaaacg gtcgtgttgt taacatcgct tcttatcagg ttagtccgaa
    13801 tgacgttgta agcattcgtg agaaagcgaa gaagcagtct cgcgtgaaag ccgctctgga
    13861 gctggctgag cagcgtgaaa agccaacctg gctggaagtt gatgctggca agatggaagg
    13921 tacgtttaag cgtaagccgg agcgttctga tctgtctgcg gacattaacg aacacctgat
    13981 cgtcgagctt tactccaagt aaagcttagt accaaagaga ggacacaatg cagggttctg
    14041 tgacagagtt tctaaaaccg cgcctggttg atatcgagca agtgagttcg acgcacgcca
    14101 aggtgaccct tgagccttta gagcgtggct ttggccatac tctgggtaac gcactgcgcc
    14161 gtattctgct ctcatcgatg ccgggttgcg cggtgaccga ggttgagatt gatggtgtac
    14221 tacatgagta cagcaccaaa gaaggcgttc aggaagatat cctggaaatc ctgctcaacc
    14281 tgaaagggct ggcggtgaga gttcagggca aagatgaagt tattcttacc ttgaataaat
    14341 ctggcattgg ccctgtgact gcagccgata tcacccacga cggtgatgtc gaaatcgtca
    14401 agccgcagca cgtgatctgc cacctgaccg atgagaacgc gtctattagc atgcgtatca
    14461 aagttcagcg cggtcgtggt tatgtgccgg cttctacccg aattcattcg gaagaagatg
    14521 agcgcccaat cggccgtctg ctggtcgacg catgctacag ccctgtggag cgtattgcct
    14581 acaatgttga agcagcgcgt gtagaacagc gtaccgacct ggacaagctg gtcatcgaaa
    14641 tggaaaccaa cggcacaatc gatcctgaag aggcgattcg tcgtgcggca accattctgg
    14701 ctgaacaact ggaagctttc gttgacttac gtgatgtacg tcagcctgaa gtgaaagaag
    14761 agaaaccaga gttcgatccg atcctgctgc gccctgttga cgatctggaa ttgactgtcc
    14821 gctctgctaa ctgccttaaa gcagaagcta tccactatat cggtgatctg gtacagcgta
    14881 ccgaggttga gctccttaaa acgcctaacc ttggtaaaaa atctcttact gagattaaag
    14941 acgtgctggc ttcccgtgga ctgtctctgg gcatgcgcct ggaaaactgg ccaccggcaa
    15001 gcatcgctga cgagtaaccg gatcacaggt taaggtttta ctgagaagga taaggtcatg
    15061 cgccatcgta agagtggtcg tcaactgaac cgcaacagca gccatcgcca ggctatgttc
    15121 cgcaatatgg caggttcact ggttcgtcat gaaatcatca agacgactct gcctaaagcg
    15181 aaagagctgc gccgcgtagt tgagccgctg attactcttg ccaagactga tagcgttgct
    15241 aatcgtcgtc tggcattcgc ccgtactcgt gataacgaga tcgtggcaaa actgtttaac
    15301 gaactgggcc cgcgtttcgc gagccgtgcc ggtggttaca ctcgtattct gaagtgtggc
    15361 ttccgtgcag gcgacaacgc gccgatggct tacatcgagc tggttgatcg ttcagagaaa
    15421 gcagaagctg ctgcagagta atctgaagca acgtaaaaaa acccgccccg gcgggttttt
    15481 ttatacccgt agcatcccca cttatctaca atagctgtac tctttttgtt catcccctgg
    15541 agtatatatg tggttacttg accagtgggc agagcgtcat atagcagaag cgcaagcgaa
    15601 aggtgagttt gataacctgg caggtagcgg cgaaccattg atactggatg atgattctca
    15661 cgtgccaccg gaattacgtg cggggtatcg cttgttgaag aatgccggtt gcttaccgcc
    15721 agaacttgag caacggaggg aagcaattca gcttctggat attctcaaag gtatccgtca
    15781 cgatgatccg caatatcaag aggttagccg tcgattgtca ttactggaat tgaagctgcg
    15841 acaagctgga ctgagtaccg attttttacg cggcgattat gctgacaagt tgttgaataa
    15901 aatcaacgat aactagtgga gtatgtatgt atcgcattgg tgagctggca aaaatggcgg
    15961 aagtaacacc cgacacgatt cgttattacg aaaaacagca gatgatggag catgaagtgc
    16021 gtactgaagg tgggtttcgc ctgtataccg aaagcgatct ccagcgattg aaatttatcc
    16081 gccatgccag acaactaggt ttcagtctgg agtcgatccg cgagttgctg tcgatccgta
    16141 tcgatcctga acaccatacc tgtcaggagt caaaaggcat tgtgcaggaa agattgcagg
    16201 aagtcgaagc acggatagcc gagttgcaga gtatgcagcg ttccttgcaa cgccttaacg
    16261 atgcctgttg tgggaccgcc catagcagtg tttattgctc gattcttgaa gctcttgaac
    16321 aaggggcgag tggcgttaag agtggctgtt gattttttgc actgacagga ttacactcgc
    16381 gccgttaaat aaccaactgg agtttttatg agccgatatc agcatactaa agggcagata
    16441 aaggataatg cgatagaagc attactacat gatcccttat tccgacagcg agtagagaaa
    16501 aataagaaag ggaaaggcag ttacatgcga aaaggaaaac atggcaatcg ggggaactgg
    16561 gaggccagtg gcaagaaagt aaatcacttt tttaccactg gccttctgct ttcaggcgtt
    16621 tgttaagagc ggttattctg ctctttcagc aaatcacgaa tttctgtcag taatacttct
    16681 tctttagttg gtgctggtgc ggctgctggt tcttcttttt tccgattcag tttgttgatt
    16741 agcttaatcg ccataaagat ggcaaaggcc acaatcagaa aatcaaagac gttttgaatg
    16801 aagacaccgt aatgcatcac aacagcaggg atatccccct gcgcatcgcg tagcgtgaca
    16861 gcaaactgtt taaagtcgat cccgccaatt aataagccca gaggtggcat gatgatatcg
    16921 gcaaccagtg aagatacaat cttcccgaat gccgcaccga taatgacacc caccgccaaa
    16981 tccaccacgt tcccgcgcat cgcaaattcg cgaaattctt taataatgct catgttattc
    17041 tccctatgaa gccgacaacc ataagtctaa caaatgttaa gccattttcc tgccaggaat
    17101 gaatttaatt aatccttgat aatcaaaggg aaataataag gcgtcattag acgccttatt
    17161 aattacaaga agaaagggct tggctggaag agtctttcga cgtcggtaat aaacttttta
    17221 tctgtgagga acataattac gtgatcgcct tgctcaatgc gcagattgtc attggcaatc
    17281 atcacgtcgt ttccacgtac caccgctcca ataatcgttc ctggcggtag cttgatttcg
    17341 tcaatgactc tgccgacaac gcgtgaggtg ctttcatcac cgtgagcaac ggcttcaata
    17401 gcttctgcta cgccgcggcg caatgaggaa acaccaacaa tatctgcttt tcgcacatgg
    17461 ctaagcaacg cagaaatagt tgcttgttgt ggtgaaatcg caatatcgat cacgctcccc
    17521 tgaaccagat ccacataagc gcgacgctgg atcaatacca tcaccttttt cgcacccata
    17581 cgtttggcaa gcatggcgga catgatattg gcctcgtcat cgttggtgac ggcaataaac
    17641 agatcaactt gatcgatatg ttcttcggcc agcagttctt gatccgacgc atcaccaaaa
    17701 aagacgatcg tattctgcaa cttttccgcc agttcggcag cgcgctgctg attacgttcg
    17761 atgagtttaa cgctgtaatc tttttccaga cgacgcgcca gccctgcacc gatattaccg
    17821 ccaccaacca gcatgatccg cttatacggt ttttccagtc gctgtaattc actcatcacc
    17881 gcacggatat gctgtgaagc ggcaataaag aacacttcat caccagcttc cacaatggtc
    17941 gaaccttgtg gacgaatggg gcgatcgtgg cggaaaattg ctgccacacg agtatcgata
    18001 tgtggcatat gttcgcgcat ggtcgaaagt gcgttaccaa tcagcgggcc gccataatag
    18061 gctttaacca cagcgaggct gactttaccc tcagcgaagt tcaccacctg caatgcgccg
    18121 ggatactcaa tcagtcggta aatattatcg ataaccaact gctctggtgc gatcagatga
    18181 tcaatcggta cagcatctga atgaaatagc ttatcggcat cgcgcacgta gtctggtgag
    18241 cggatgcgag cgatgcgatt aggggtgttg aaaagtgagt aggctacctg gcaggcaacc
    18301 atattggttt catctgaact ggttacagca accagcatat cggcgtcgtc ggcacctgcc
    18361 tcccgcaata cgcgtggatg agagccatgc ccctgcacga ctcgcaggtc aaatttatcc
    18421 tgcaaggtcc gcagacgctc accgttggta tcgacaacag tgatatcgtt gttctcgcca
    18481 accaggtttt ccgccagtgt gccgccaacc tggccggcac ccagaatgat aattttcatc
    18541 agtcgcgacc cgttatctca tcactttttg attagcttag cgtaaaagaa gccgtcgccc
    18601 tcttcggcac caggcagatt ttgtttaccc ggttgctctg gtgttcctgt ttcgcaaagt
    18661 tcggcatcag cggtacgttg caaaaaggct ttaatctgca ggctattctc ttccggtaac
    18721 accgaacagg tggcatagac cagggttcca ccggatttta aatgcggcca aatggcgtcg
    18781 agaatttcag actgcaattg cgcgagttcc gggatatcgc gatcgcgacg taaccattta
    18841 atatccggat ggcgacgaat cacaccggtt gctgaacaag gcgcatctaa taaaatgcga
    18901 tcaaactgtt gctcgccaca ccattgggaa gggtagcggc catcaccttg tttcacggtc
    18961 gctttcatac caaggcgttt taaattgtcg taaacgcgag agaggcgctg ttcgtcaata
    19021 tcaaccgcaa caacctgcgc ttctggtgcc acctcaagga tatgcgttgt tttaccgccg
    19081 ggggcggcac aaagatccag aatgtgttca ccgttttgtg gcgcaagcca ggtcatgcaa
    19141 ccttgtgctg atgcatcctg aacggtaacc catccgtctt caaaaccagg tagcgcatga
    19201 acaggtgcag gtgtttccag ccgtacagca tcagggtaat ccgcatgcgg gaaacctttc
    19261 attcctgctt catccaacaa tgcaagccag ctgtcgcggg aatgatgcgt acgattaacg
    19321 cgcagccaca ttggcggacg ctggttattg gcttcgacga tggattgcca ctgctctgga
    19381 tacgcttttt gcagacgctt cagcaaccag gaaggatgca gataacgtgc atcactggca
    19441 ttaaactcgg ctaataactc ttcttgctga cgctggaact ggcgtaatac accgtttatc
    19501 aaccctttaa gttgcggacg cttaattgcg atagcgcctt caaccgtttc agccagcgca
    19561 gcatgaggtg gaatgcgggt ataaagcagt tgatacaaac caaccataat caggtaatgc
    19621 acggtccgct gtttgccggt catcggacgg gccattaact tattaatcag ccagtctaac
    19681 tgcgaaagcg tacgcagtac gccaaagcac aactcttgaa gaagtgcttt gtctttatcg
    19741 gaaacttttt gctggagcgg tggcagaatg ttgcttaatg attgcccttg ctcgacgact
    19801 tgttcaacgg cctgggccgc catgctacgt aaattacgtt gttttttcat aagtataaaa
    19861 atgcccggca agaccgggct tagaagagtg gactatcaga ccagacggtt gcccggaaca
    19921 aaccattccc gacgagagtt caggaggtct tgcgcgctca tcgctttctt acccgcaggt
    19981 tgtaacgaga gcaggttcag gatgccatca ccagtcgcaa cctgaatgcc ttgtttattg
    20041 gcttcaagga tcgttcctgg tgcagcgttg gttgccgtat caatgaccga cgctttccag
    20101 actttaaccg gttgtccttc aatttccagc cagctcattg gccatgggtt gaaagcgcga
    20161 atgcagcgtt caagctgcgc tgccgaaagc gaccagtcaa tacgcgcttc ttctttactc
    20221 aacttctcgg cgtaagtgac aagagtttcg tcctgaactt ctggtttcgc cgtgccgtct
    20281 gccagttgtt tcaacgtggt gataagccct tgtgggccaa gctctgccag cttgtcgtac
    20341 agcgtaccac tggtatcttc tgcagtaatc gggcaggaga gcttatagag catgtcaccg
    20401 gtgtctaaac caacatccat ttgcataatg gtcacaccag tttctgcatc acccgcccat
    20461 agtgagcgtt ggattggtgc agcaccgcgc cagcgtggca gcagtgaacc atgaacgttg
    20521 atacagccaa gacgcggcat ctccagcaca gctttaggca gaattaaacc ataggcgacg
    20581 acgaccataa catcagcctg cagatcggcg accagttgct ggttttcttg tggacgcagg
    20641 gaaacaggtt gaaaaacggg cagacctttt tcctcagcca gaactttaat cgggctgggc
    20701 atcagttttt taccgcgtcc tgccggtcgg tctggctggg tgaacacgcc aacgacgtta
    20761 tgaccagaag acaacagcgc gtcgagatga cgcgctgcaa agtcaggtgt acccgcaaaa
    20821 ataatacgta gtgattctga cacgttagtt cttatcctta agcccgggct ttcagacgat
    20881 ccagtttttc aactttctga cgaatacgtt gttgtttcag cggtgacaga taatccataa
    20941 acagtttgcc gaccaggtga tccatctcat gctgaataca gatggctaac agaccatctg
    21001 cctccagttc aaatggttta ccgtcgcggt ccagggcgcg aattttaact ttctctgcgc
    21061 gtggcactaa agcacgttgt tcagggatcg acaggcaacc ttcttcaatg cctgtttcgc
    21121 cgcttttttc taaaagctcc ggattgatta acaccagccg ttcgtcacgg ttttccgaaa
    21181 catcaataac aatgatacgt tgatggatat caacctgggt tgccgccagg ccaatacctt
    21241 cttctgcata catcgtctcg aacatatcat cgacgatacg ctgaatttct gcattcactt
    21301 cttctaccgg tttagcaact ttgcgaagcc gctcgtccgg aatatgtaac acttgcaaaa
    21361 ctgacataaa tctccagaga tgtgttcagg agttagaaag attatttctt ctattctaga
    21421 caaatccccc tctgattgac agcatcactg accaatcgca aagattgcta aggctgctta
    21481 tggcagggag ataaggatgg tcgatacaga aatttggctg cgtttaatga gtatcagcag
    21541 cttgtacggc gatgatatgg tccgtatagc tcactggctg gcaaaacagt cgcatattga
    21601 tgcggttgta ttgcagcaaa ctgggcttac attgcggcag gcacaacgct ttctttcatt
    21661 tccgcgaaag agtatcgaaa gctcactttg ttggttggag caacccaacc atcatttaat
    21721 ccctgcggac agcgaatttt atcctcctca acttcaggcg acgacagatt accccggcgc
    21781 actgtttgtt gaaggagaac tgcacgcgct gcattcattt cagcttgcca tagtggggag
    21841 tcgggcgcat tcatggtatg gcgagcgatg gggacgatta ttttgcgaaa ctctggcgac
    21901 gcgtggagtg acaattacaa gtggactggc gcgtggaatc gatggtgtgg cgcataaagc
    21961 ggccttacag gtaaatggcg tcagcattgc tgtattgggg aatggactta ataccattca
    22021 tccccgccgc catgcccgac tggctgccag tttgcttgaa catggcggag ctctcgtctc
    22081 ggaatttccc ctcgatgttc caccccttgc ttacaatttc ccacgaagaa atcgcattat
    22141 cagtggtcta agtaaaggtg tactggtggt ggaagcggct ttgcgcagtg gttcgctagt
    22201 gacggcacgt tgtgcgcttg agcaggggcg tgaagttttt gccttgccag gtccaatagg
    22261 gaatccggga agcgaagggc ctcactggtt aataaaacaa ggtgcgattc ttgtgacgga
    22321 accggaagaa attctggaaa acttgcaatt tggattgcac tggttgccag acgcccctga
    22381 aaattcattt tattcaccag atcagcaaga cgtggcattg ccatttcctg agctcctggc
    22441 taacgtagga gatgaggtaa cacctgttga cgtcgtcgct gaacgtgccg gccaacctgt
    22501 gccagaggta gttactcaac tactcgaact ggagttagca ggatggatcg cagctgtacc
    22561 cggcggctat gtccgattga ggagggcatg ccatgttcga cgtactaatg tatttgtttg
    22621 aaacctatat tcacacagaa gctgagttgc gtgtggatca agacaaactt gaacaggatc
    22681 ttaccgacgc aggatttgat cgagaagata tctacaatgc cctgctttgg ctggaaaagc
    22741 ttgcggatta tcaggaagga ctggcagaac cgatgcaact ggcctctgat ccactctcca
    22801 tgcgtattta tacccctgag gagtgtgaaa gactggatgc cagctgtcgt ggttttctgc
    22861 ttttccttga gcagattcag gtgctcaacc ttgaaactcg tgaaatggtg atagagcgag
    22921 tgctcgcgct ggataacgca gagttcgaac tggatgatct gaaatgggta atcctgatgg
    22981 tgttgttcaa tattccgggc tgcgaaaatg cgtaccagca aatggaagaa ttactctttg
    23041 aagtgaatga aggtatgctg cattaatcat ttctttaatt cagcataagt tgttatggcg
    23101 aaatcagcac tgttcacggt gcgtaataat gagtcctgcc caaagtgcgg ggctgaactg
    23161 gttattcgat ccgggaaaca cggtccgttt cttggatgct cacagtatcc ggcgtgtgac
    23221 tacgtccgtc ctctgaaatc ttcagcggat ggacatattg tcaaagttct ggaggggcag
    23281 gtttgccctg catgtggcgc aaatctggta ttacgccagg gacgctttgg tatgtttatt
    23341 ggttgcagta actaccctga atgcgaacat accgaactta tcgataaacc ggacgaaaca
    23401 gcaattacat gcccccaatg ccggacgggc catctggtcc agcgccgctc ccgttatggc
    23461 aaaacatttc actcctgtga tcgctacccg gagtgtcaat ttgccattaa cttcaaaccc
    23521 atagctggag aatgccctga gtgtcattat ccactactca tcgaaaagaa aaccgcgcag
    23581 ggtgtaaaac acttttgtgc cagtaaacaa tgtggaaagc cggtttcggc ggaataataa
    23641 cgtgaataat aacctgcaag gagacgctat cgcagctgcg atagatgtcc tcaatgaaga
    23701 acgtgtcatc gcctatccaa cggaagccgt tttcggtgtc gggtgcgatc ctgatagcga
    23761 aacagcagtg atgcgactgt tggagttaaa acagcgtccg gttgataagg ggctgatttt
    23821 aatcgcggca aattacgagc agcttaaacc ctatattgat gacaccatgc tgactgatgc
    23881 gcagcgtgaa accatttttt cccgctggcc aggtcctgtc acctttgtct ttcccgcgcc
    23941 tgcgacaaca ccgcgctggt tgacgggccg ctttgattcg cttgctgtac gagtcaccga
    24001 ccatccgttg gtggttgctt tgtgccaggc ttatggtaaa ccgctggttt ctaccagtgc
    24061 caacttgagt ggattgccac cttgtcgaac agtagacgaa gttcgcgcac aatttggcgc
    24121 ggcgttcccg gttgtgcctg gtgaaacggg ggggcgttta aatccttcag aaatccgcga
    24181 tgccctgacg ggtgaactgt ttcgacaggg gtaacataat ggaaacctat gctgtttttg
    24241 gtaatccgat agcccacagc aaatcgccat tcattcatca gcaatttgct caacaactga
    24301 atattgaaca tccctatggg cgcgtgttgg cacccatcaa tgatttcatc aacacactga
    24361 acgctttctt tagtgctggt ggtaaaggtg cgaatgtgac ggtacctttt aaagaagagg
    24421 cttttgccag agcggatgag cttactgaac gggcagcgtt ggctggtgct gttaataccc
    24481 tcaagcggtt agaagatgga cgcttgcagg gtgacaatac cgatggtata ggtttgttaa
    24541 gcgatctgga acgtctgtct tttatccgtc cgggtttacg tattctgctt atcggcgctg
    24601 gtggagcatc tcgcggcgta ctactaccac tcctttccct ggactgtgcg gtgacaatca
    24661 ctaatcggac ggtatcccgc gcggaagagt tggctaaatt gtttgcgcac actggcagta
    24721 ttcaggcgtt gggtatggac gaactggaag gtcatgagtt tgatctcatt attaatgcaa
    24781 catccagtgg catcagtggt gatattccgg cgatcccgtc atctctcatt catccaggca
    24841 tttattgcta tgacatgttc tatcagaaag gaaaaactcc ttttctggca tggtgtgagc
    24901 agcgaggctc aaagcgtaat gctgatggtt taggaatgct ggtggcacag gcggctcatg
    24961 cctttcttct ctggcacggt gttctgcctg acgtagaacc agttataaag caattgcagg
    25021 aggaattgtc cgcgtgaatc aggccatcca gtttccggac agagaagagt gggacgagaa
    25081 taaaaaatgt gtatgttttc ccgctctcgt gaatggcatg caactgacat gtgcgatctc
    25141 tggcgagagt ctggcgtatc gttttactgg agatacgcca gaacagtggt tagcgagttt
    25201 tcgtcagcat cgctgggatc tggaagaaga agcggaaaac ttaattcagg aacaaagtga
    25261 agatgatcaa ggctgggtct ggttaccctg atccagatat tcgtccttcc atttcacgta
    25321 attattcgcg gaatagcgta acccagcctt ctcttcatca cttaacgggc ggatctgttt
    25381 gacggggcta ccgagataca gatatccgct ctccagccgt ttattttgtg ggaccagact
    25441 acccgcacca atcatcacat catcttctac tattgcgcca tcaagtaaaa ttgagcccat
    25501 cccaaccaaa actcgattgc caatggtgca gccgtggagc atcaccttgt gaccaacagt
    25561 gacatcttcg ccaatggtta atgggttgcc atctgggttg tacgaggatt tatgagtgac
    25621 atgcaacata ctgccatcct ggatattggt gcgtgctccg atctgtacat aatgtacatc
    25681 tccacgaatc acaacgagcg gccagatccc cacatcatca gccagacgaa cgtcaccaat
    25741 cacgacactg ctatcgtcga tcattacgcg ctgaccgatt tgtggaaaaa gatcgcggta
    25801 tgggcgtaaa acatcagaca tacttacctc agcaataaat gatttactaa tgactttggg
    25861 ggcattattg gccttgtgca agtcttttag tatgcaaaaa agcaccgttt tgtgtgcgat
    25921 tgcagcaaaa agggtgaaaa aacaacaaac agaaaaaaag atcaaaaaaa tacttgtgca
    25981 aaaaattggg aaccctataa tgcgcctccg ttgagacgac aacgtgaaac acttcacagg
    26041 atggtcggaa caacgaagag
//
LOCUS       DAZFQD010000066        25309 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.066, whole genome shotgun sequence.
ACCESSION   DAZFQD010000066 DAZFQD010000000
VERSION     DAZFQD010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25309
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.066"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            163..510
                     /gene="grxD"
                     /locus_tag="ACHKTW_002756"
     CDS             163..510
                     /gene="grxD"
                     /locus_tag="ACHKTW_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HFO6463754.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            complement(561..5177)
                     /locus_tag="ACHKTW_002757"
     CDS             complement(561..5177)
                     /locus_tag="ACHKTW_002757"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HFO6463755.1"
                     /translation="MADNPDPSSLLPDVFSPATRDWFLRAFKQPTAVQSQTWHVAARS
                     EHALVIAPTGSGKTLAAFLYALDRLFREGGEDTREAHKRKTSRILYISPIKALGTDVQ
                     RNLQLPLKGIADERRRRGETEVNLRVGIRTGDTPAQERSKLTRNPPDILITTPESLYL
                     MLTSRARETLRGVETVIIDEVHAVAGSKRGAHLALSLERLDALLHTSAQRIGLSATVR
                     SASDVAAFLGGDRPVTVVNPPAMRHPQIRIVVPVANMDDVSSVASGTGEDSHAGREGS
                     IWPYIETGILDEVLRHRSTIVFTNSRGLAEKLTARLNELYAARLQRSPSIAVDTAHFE
                     STSGATSNHVQSSDVFIARSHHGSVSKEQRAITEQALKSGELRCVVATSSLELGIDMG
                     AVDLVIQVATPLSVASGLQRIGRAGHQVGGVSKGLFFPRTRRDLVDSAVIVECMFAGR
                     LENLTPPHNPLDVLAQQTVAAAAMDALQVDEWYSRVRRAAPWKDLPRRVFDATLDMLS
                     GRYPSGDFSAFRPKLVWNRETGILTARPGAQLLAVTSGGTIPDRGMYSVLLPEGEEQA
                     GSRRVGELDEEMVYESRVNDIITLGATSWRIQQITRDQVIVTPAPGRSARLPFWRGEG
                     NGRPAELGEMIGDFLHLLADGAFFSGTIPPWLAEENTNANIQGLIDEQRNATGIVPGS
                     RHLVLERCRDEIGDWRIILHSPYGRRVHEPWALAIAGRIHALWGADASVVASDDGIVA
                     RIPDTDGKLPDAAIFLFEPEKLLQIVREAVGSSALFAARFRECAARALLMPGRTPGHR
                     TPLWQQRLRASQLLEIAQGYPDFPVILETLRECLQDVYDLPALERLMRRLNGGEIQIS
                     DVTTTTPSPFATSLLFGYVAEFMYQSDAPLAERRASVLSLDSELLRNLLGQVDPGELL
                     DPQVIRQVEEELQRLAPGRRAKGEEGLFDLLRELGPMTVEDLAQRHTGSSEEVASYLE
                     NLLAVKRIFPAMISGQERLACMDDAARLRDALGVRLPESLPEIYLHRVSYPLRDLFLR
                     YLRAHALVTAEQLAHEFSLGIAIVEEQLQQLREQGLVMNLQQDIWVSDEVFRRLRLRS
                     LQAAREATRPVAATTYARLLLERQGVLPATDGSPALFASTSPGVYEGVDGVMRVIEQL
                     AGVGLPASLWESQILPARVRDYSSEMLDELLATGAVIWSGQKKLGEDDGLVALHLQEY
                     AAESFTPAEADQANRSALQQAIVAVLADGGAWFAQQISQRIRDKIGESVDLSALQEAL
                     WALVWQGVITSDIWAPLRALTRSSSNARTSTRRSHRARRGRPVYAQPVSPLVSYNTPN
                     LAGRWSLLQVEPLNDTERMLALAENMLDRYGIISRQAVIAENIPGGFPSMQTLCRSME
                     DSGRIMRGRFVEGLGGAQFAERLTIDRLRDLATQATQTRHYTPVALSANDPANVWGNL
                     LPWPAHPATLVPTRRAGALVVVSGGKLLLYLAQGGKKMLVWQEKEELLAPEVFHALTT
                     ALRREPRLRFTLTEVNDLPVRQTPMFTLLREAGFSSSPQGLDWG"
     gene            complement(5270..5917)
                     /gene="rnt"
                     /locus_tag="ACHKTW_002758"
     CDS             complement(5270..5917)
                     /gene="rnt"
                     /locus_tag="ACHKTW_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - RNA exonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HFO6463756.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            complement(6020..6427)
                     /gene="gloA"
                     /locus_tag="ACHKTW_002759"
     CDS             complement(6020..6427)
                     /gene="gloA"
                     /locus_tag="ACHKTW_002759"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFO6463757.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGHGLGN"
     gene            complement(6508..7605)
                     /gene="nemA"
                     /locus_tag="ACHKTW_002760"
     CDS             complement(6508..7605)
                     /gene="nemA"
                     /locus_tag="ACHKTW_002760"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HFO6463758.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMAVQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAGFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRVSPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQRKAELNPQRAESFYGGGAEGYTDYPTL"
     gene            complement(7642..8241)
                     /gene="nemR"
                     /locus_tag="ACHKTW_002761"
     CDS             complement(7642..8241)
                     /gene="nemR"
                     /locus_tag="ACHKTW_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416166.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HFO6463759.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYAAYHQRLTELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRSFEPLENALAHVKNIIATPAV"
     gene            8344..8583
                     /locus_tag="ACHKTW_002762"
     CDS             8344..8583
                     /locus_tag="ACHKTW_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HFO6463760.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPKQPSLF"
     gene            8632..9528
                     /locus_tag="ACHKTW_002763"
     CDS             8632..9528
                     /locus_tag="ACHKTW_002763"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HFO6463761.1"
                     /translation="MVQRITIAPQGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RDHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            9609..10130
                     /gene="sodC"
                     /locus_tag="ACHKTW_002764"
     CDS             9609..10130
                     /gene="sodC"
                     /locus_tag="ACHKTW_002764"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC"
                     /protein_id="HFO6463762.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGAGHLGDLPALVVNNDGKATDAVIAPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(10131..12143)
                     /locus_tag="ACHKTW_002765"
     CDS             complement(10131..12143)
                     /locus_tag="ACHKTW_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFO6463763.1"
                     /translation="MNASSWSLRNLPWFKATLAQWRYALRNTIAMCLALTVAYYLNLD
                     EPYWAMTSAAVVSFPTVGGVISKSLGRIAGSLLGAIAALLLAGHTLNEPWFFLLSMSA
                     WLGFCTWACAHFTNNVAYAFQLAGYTAAIIAFPMVNITEASQLWDIAQARVCEVIVGI
                     LCGGMMMMILPSSSDATALLTALKNMHARLLEHASLLWQPETNDAIRAAHEGVIGQIL
                     TMNLLRIQAFWSHYRFRQQNARLNALLHQQLRMTSVISSLRRMLLNWPSPPGATREIL
                     EQLLTALASSQTDAYTVARIIAPLRPTNVADYRHVAFWQRLRYFCRLYLQSSQELHRL
                     QSGVDDHARLARTSALARHTDNTEAMWSGLRTFCTLMMIGAWSIASQWDAGANALTLA
                     AISCVLYSAVAAPFKSLSLLMRTLVLLSLFSFVVKFGLMVQISDLWQFLLFLFPLLAT
                     MQLLKLQMPKFAALWGQLIVFMGSFIAVTNPPVYDFADFLNDNLAKIVGVALAWLAFA
                     ILRPGSDARKSRRHIRALRRDFVDQLSRHPTLSESEFESLTYHHVSQLSNSQDALARR
                     WLLRWGVVLLNCSHVVWQLRDWESRSDPLSRVRDNCISLLRGVMSERGVQQKSLAATL
                     EELQRICNSLARHHQPAARELAAIVWRLYCSLSQLEQAPPQGTLAS"
     gene            complement(12143..13000)
                     /locus_tag="ACHKTW_002766"
     CDS             complement(12143..13000)
                     /locus_tag="ACHKTW_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HFO6463764.1"
                     /translation="MSIKTIKYFSTIIVAIVAVLAGWWLWNYYMQSPWTRDGKIRAEK
                     VSITPQVSGRIVELNIKDNQLVNAGDLLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFISAEELDTANLNVKAMQASVNAAQATLKQTQWQLAQTEIRAPVSG
                     WVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LRGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQRVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            complement(13003..13239)
                     /locus_tag="ACHKTW_002767"
     CDS             complement(13003..13239)
                     /locus_tag="ACHKTW_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HFO6463765.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            13440..13874
                     /gene="slyA"
                     /locus_tag="ACHKTW_002768"
     CDS             13440..13874
                     /gene="slyA"
                     /locus_tag="ACHKTW_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HFO6463766.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            complement(13921..14388)
                     /gene="slyB"
                     /locus_tag="ACHKTW_002769"
     CDS             complement(13921..14388)
                     /gene="slyB"
                     /locus_tag="ACHKTW_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HFO6463767.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSTGQRVVLASNGSQVTVSPR"
     gene            14662..15771
                     /gene="anmK"
                     /locus_tag="ACHKTW_002770"
     CDS             14662..15771
                     /gene="anmK"
                     /locus_tag="ACHKTW_002770"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HFO6463768.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHST
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAALLPGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANP"
     gene            15869..16198
                     /gene="mliC"
                     /locus_tag="ACHKTW_002771"
     CDS             15869..16198
                     /gene="mliC"
                     /locus_tag="ACHKTW_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HFO6463769.1"
                     /translation="MSMKKLLLICLPVLLTGCSAFNQLVEHMQTATLEYQCDEKPLTV
                     KLNNPRQEVSFVYDNQLLHLKQGISASGARYTDGIYVFWSKGDEATVYKRDRIVLNNC
                     QLQNPQR"
     gene            16257..16913
                     /gene="pdxH"
                     /locus_tag="ACHKTW_002772"
     CDS             16257..16913
                     /gene="pdxH"
                     /locus_tag="ACHKTW_002772"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HFO6463770.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHAQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            17042..18316
                     /gene="tyrS"
                     /locus_tag="ACHKTW_002773"
     CDS             17042..18316
                     /gene="tyrS"
                     /locus_tag="ACHKTW_002773"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFO6463771.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYGVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            18375..19238
                     /gene="pdxY"
                     /locus_tag="ACHKTW_002774"
     CDS             18375..19238
                     /gene="pdxY"
                     /locus_tag="ACHKTW_002774"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HFO6463772.1"
                     /translation="MMKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNH
                     TQYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKA
                     ANPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVNN
                     VEEAVLAARELIAQGPQIVLVKHLARAGYSRDRFEMLLVTADEAWHISRPLVDFGMRQ
                     PVGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIA
                     KPEHYFSATKL"
     gene            complement(19282..19887)
                     /gene="gstA"
                     /locus_tag="ACHKTW_002775"
     CDS             complement(19282..19887)
                     /gene="gstA"
                     /locus_tag="ACHKTW_002775"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HFO6463773.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     VVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKPTVRAQLEKKLQYVNEALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(19993..21495)
                     /gene="dtpA"
                     /locus_tag="ACHKTW_002776"
     CDS             complement(19993..21495)
                     /gene="dtpA"
                     /locus_tag="ACHKTW_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HFO6463774.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPINY
                     RNLLLTIIGVVALIAIATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAIIAVLMLLTAPKLHRMTQDDAADKAAKAAVA"
     gene            complement(22107..22742)
                     /gene="nth"
                     /locus_tag="ACHKTW_002777"
     CDS             complement(22107..22742)
                     /gene="nth"
                     /locus_tag="ACHKTW_002777"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFO6463775.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     VSVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(22742..23437)
                     /gene="rsxE"
                     /locus_tag="ACHKTW_002778"
     CDS             complement(22742..23437)
                     /gene="rsxE"
                     /locus_tag="ACHKTW_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HFO6463776.1"
                     /translation="MSEIKDVIVQGVWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGMGATRAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRAEAT
                     AERALPNGETGNV"
     gene            complement(23441..24061)
                     /gene="rsxG"
                     /locus_tag="ACHKTW_002779"
     CDS             complement(23441..24061)
                     /gene="rsxG"
                     /locus_tag="ACHKTW_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HFO6463777.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQEA
                     LFDQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWITHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            complement(24065..25123)
                     /gene="rsxD"
                     /locus_tag="ACHKTW_002780"
     CDS             complement(24065..25123)
                     /gene="rsxD"
                     /locus_tag="ACHKTW_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HFO6463778.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGLWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLIFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            complement(25124..>25309)
                     /locus_tag="ACHKTW_002781"
     CDS             complement(25124..>25309)
                     /locus_tag="ACHKTW_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001439589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transporter RnfC"
                     /protein_id="HFO6463779.1"
                     /translation="VDPRKAAVEAAIARAKARKLEQQQTNAEPEEQVDPRKAAVAAAI
                     ARAQAKKAAQQKVVNED"
BASE COUNT         6290 a         6539 c         6604 g         5876 t
ORIGIN      
        1 ccagtttgcc gcgcatcaga gaagaaacga atatttgaaa tgcaacaaat agagcaaagg
       61 gataaaatag caaaagcgtg atgaaaatgt tattttccga tgccgcactg acccgctaca
      121 atgtcagatt actgccgtaa agaagtttga ggaagcaaga caatgagcac cactatcgaa
      181 aaaatccaac gccagattgc tgaaaacccg atcctgctgt acatgaaagg ttcaccgaaa
      241 ctgccgagct gcggtttctc tgcccaggca gtccaggcgc ttgccgcatg tggcgaacgt
      301 tttgcctatg ttgatattct gcagaatccg gacattcgtg cggaactgcc gaaatatgct
      361 aactggccga ccttcccgca actgtgggtt gacggcgagc tggtcggcgg ttgtgatatc
      421 gtgatcgaaa tgtatcagcg tggcgaactg cagcagctga tcaaagaaac tgccgctaaa
      481 tacaagtctg aagagccgga cgcggaataa tccgtcctgc aatactgacg gatgcgaacg
      541 ggcatccgtc agtcctttct ctatccccaa tccagccctt gtggcgaact tgaaaatccc
      601 gcctcacgca gcagcgtaaa catcggcgtt tgccggaccg gtagatcatt cacttctgtt
      661 agcgtaaagc gcagccgtgg ttcgcgacgc agtgcggtag tcagcgcgtg gaaaacctct
      721 ggggcgagta attcctcttt ttcctgccag accagcattt ttttgccacc ttgcgccaga
      781 tagagtaaca atttgccgcc agaaacgacc accagcgcac ccgcccgacg cgttggaacc
      841 agcgttgccg gatgtgcagg ccagggcaga agatttcccc acacattagc cggatcgttg
      901 gcagagagcg ccactggtgt atagtggcgc gtttgcgtgg cttgtgtcgc cagatcgcgc
      961 aatcggtcaa tagtcagacg ttcagcgaat tgcgcgccac ccagaccttc tacaaaacga
     1021 cctcgcataa ttcgcccgga gtcttccata cttcgacaaa gcgtttgcat cgatggaaac
     1081 ccgccaggga tattttcggc tatcaccgcc tgacgactga tgatgccgta gcggtcgagc
     1141 atattttccg ccagcgccag catcctttcg gtatcgttta gtggctccac ctgcaataac
     1201 gaccagcgtc cagccagatt tggtgtgttg taagatacca gcggcgagac gggttgcgca
     1261 tagacaggac gtccacgacg agcccggtga ctgcggcgag ttgaggtgcg tgcgttggaa
     1321 ctgctgcggg tgagggcgcg taacggtgcc caaatgtcgc tggtgatgac gccttgccag
     1381 accagcgccc ataacgcctc ttgcagggca gagagatcaa ccgattcgcc gattttgtcg
     1441 cgtatccgtt ggctgatttg ttgtgcaaac caggctcctc cgtcagccag aacagcgact
     1501 atcgcttgtt gcagcgccga acgattcgcc tgatccgctt cggcgggagt gaacgattct
     1561 gcagcatatt cctgtagatg cagtgccacc aggccgtcat cttcacccag ttttttttgc
     1621 cccgaccaga taaccgcacc ggttgccagt aattcatcga gcatttctga cgaatagtcg
     1681 cgtacgcggg caggcaggat ctggctttcc cagagtgagg cgggtaaacc gactccggca
     1741 agctgttcga tcacccgcat cacgccatct acgccctcat aaacgcctgg cgatgttgag
     1801 gcaaagagcg ccgggctacc atcggtggcg ggtaatacgc cctgacgttc cagcagcaat
     1861 cgcgcatagg tcgtggctgc aacgggacgc gtcgcttctc tggcggcttg cagcgagcgc
     1921 aaacgcagac gacgaaatac ttcatcgctc acccagatgt cttgttgcag attcatcacc
     1981 agaccctgtt cacgcagttg ctgaagctgc tcttcgacaa tggcaatacc gagactaaac
     2041 tcatgagcca gttgttcagc cgtgaccaga gcatgagccc ggagatagcg cagaaagaga
     2101 tcgcgaagcg ggtaactgac tctatgtaaa taaatctctg gcaatgactc tggtagtcgt
     2161 acgccgaggg catcacgcag cctggcggca tcatccatac aggcaagacg ctcctgtccg
     2221 ctaatcatcg ctgggaagat tcgttttact gcaagaagat tttccagata cgacgcaacc
     2281 tcttcactgc tgcctgtatg ccgttgcgcc aggtcttcaa cggtcattgg ccccagttcg
     2341 cgcagcaggt cgaacaatcc ttcttcacct ttcgctcttc tgccaggagc cagtcgttgc
     2401 aactcttctt ccacctggcg aatgacctgc gggtcgagta attcccccgg atcgacctgt
     2461 ccgagtagat tgcgcagtaa ctcgctgtcc agcgacagta cggatgcccg gcgctctgcc
     2521 agcggggcgt cgctctggta cataaattcc gcgacatagc cgaacaataa acttgtggcg
     2581 aaaggcgagg gcgtagtggt cgttacatcg gatatttgaa tttcgccacc gttcaggcga
     2641 cgcatcaaac gttccagtgc gggaagatca taaacatctt gcagacattc gcgtagggtt
     2701 tcgagaatga ccggaaaatc cggatatccc tgagcgattt ccagcaactg actggcgcgc
     2761 agccgttgtt gccaaagcgg ggtgcgatgg cccggagtgc gccccggcat taataatgcc
     2821 cgcgcggcgc attcgcgaaa acgggcggcg aaaagtgccg agctgcctac cgcctcgcgg
     2881 acaatttgca gcaacttttc tggttcaaac aaaaaaatcg cggcgtcggg caatttacca
     2941 tcagtgtcag gaatgcgcgc aacaatgccg tcatcactgg cgaccacaga cgcgtcagcg
     3001 ccccataggg catgtattcg cccggcaatc gccagcgccc agggttcatg cacccgtctt
     3061 ccatagggag agtgcaaaat aatacgccag tcgccaattt catcacggca ccgttcgagg
     3121 accagatggc gactccccgg aacgatgccc gtcgcgttgc gttgctcgtc aatcaacccc
     3181 tgaatattgg cgttcgtatt ttcttctgcc agccacgggg gaatagtccc ggaaaagaac
     3241 gcgccatccg ccagtaaatg aagaaaatcg ccgatcatct cgcctaattc agccggacgt
     3301 ccgttacctt caccacgcca gaaggggagc cgggcagaac gacccggagc aggagtcaca
     3361 atcacctgat cgcgggtgat ttgctggatc cgccatgagg tagcgccgag agtgataatg
     3421 tcgttcaccc gcgactcata taccatctcc tcatccagtt cacccacccg ccgcgaaccg
     3481 gcctgttctt caccttcggg taataacacg ctatacatgc cacgatccgg aatggtgccg
     3541 ccgctggtaa ccgccaacaa ttgagcgcca ggtcgggcgg tcaatatccc ggtctccctg
     3601 ttccagacca gtttggggcg aaaagcagaa aaatcgccag agggatagcg cccggaaagc
     3661 atatccagcg tggcgtcaaa aacacgtctt ggcagatctt tccacggtgc ggcacggcgt
     3721 acgcgggagt accattcgtc tacctgtaat gcatccatcg ccgcggcggc aacggtttgc
     3781 tgcgcaagga cgtcgagtgg attatgcggc ggtgtcaggt tttccagcct gcctgcgaac
     3841 atacactcta caatgactgc ggaatcgact aagtcacgcc gggtacgagg gaaaaacagc
     3901 cctttagata caccgccaac ctgatgtccg gcgcgaccaa tgcgttgtaa cccactggca
     3961 acagaaagcg gcgttgccac ctgaatcacc agatccaccg cgcccatatc aatccccagt
     4021 tcgagactgg aggttgcgac cacgcagcgt aattcacccg atttcagcgc ctgttcggtg
     4081 attgctcgtt gttctttaga gacggagccg tggtgtgagc gggcaataaa aacgtcgcta
     4141 ctttgtacat ggttagaggt tgcgccggag gtcgactcga aatgggccgt atcaacggcg
     4201 atagacgggg aacgctgtaa gcgtgcggcg taaagctcat ttaatcgtgc cgtcagtttt
     4261 tccgccagcc cacgtgaatt agtaaagaca atggtcgagc gatggcgcaa cacttcatca
     4321 aggatacccg tttcaatata tggccagatg gagccttccc ggccggcatg gctgtcttcg
     4381 ccggtgccgc tggcgaccga tgagacatca tccatattgg cgaccggtac gacaattcgt
     4441 atctgcggat ggcgcattgc gggcgggttg actaccgtaa ccgggcgatc gccaccaaga
     4501 aatgctgcca catcgctggc tgagcgcaca gtggcagaaa ggccaattcg ctgtgctgag
     4561 gtgtggagca gcgcatcgag ccgctccaga cttaacgcca gatgcgcacc acgtttactg
     4621 cccgctaccg cgtggacttc atcaataatt accgtttcga cgccgcgtag cgtttcgcgc
     4681 gcgcgggagg tcagcatcag atagagtgat tcgggtgtgg tgatcagaat atccggcgga
     4741 ttacgggtga gtttgctgcg ttcctgtgca ggcgtatcgc cagtacggat ccctacgcga
     4801 agattgactt ccgtttcgcc gcgccgccgc cgttcatcgg caataccctt caacgggagc
     4861 tgcaagttgc gctgaacgtc ggtgcccaga gcttttatcg gtgaaatata gaggatgcgt
     4921 gaggttttac gcttatgcgc ctcgcgggta tcttcgccgc cttcgcggaa gagccgatcg
     4981 agggcgtaga gaaatgctgc cagcgttttc ccggagccgg tcggtgcaat caccagcgca
     5041 tgttcgcttc gcgccgccac atgccaggtt tgcgactgga cagcggtcgg ctgtttaaag
     5101 gcgcgaagaa accagtcgcg ggtcgccggt gaaaacacgt ccggcaggag cgatgaaggg
     5161 tctggattat ctgccattaa cgctattccc cgtgaatatc atgcttgatg cgtggaatag
     5221 cctcactata gatgcctgaa tcattgcatg tcatcaggca tcgactcgat tacacctctt
     5281 cggcggcagg tagcggccag cctcccagac gtttccagcg gttgacgatt tcacaaaaca
     5341 gcacagcagt gcgttcggtg tcgtacagcg cggagtgcgc ctgggtgctg tcgaagtcca
     5401 tgccagcggt ctggcaagcc tttgacaata cggtttgtcc gagtgccagc ccggccagtg
     5461 cagcagtgtc aaaagtggcg aaagggtgga acgggttacg tttcagtgag gcgcgttctg
     5521 cggcggccat cataaagctg tgatcaaaat tggcattgtg cgccaccata atggcgcggt
     5581 tacagccgct cgctttaata cctttacgta caactttaaa aatttcgtgc agcgcctcgt
     5641 attcgctgac cgcgccgcga tcgggatcgt tcgggtcaat gccgttgaag gcgagggcct
     5701 ctggctgcaa atttgcgccg acaaatggtt cgacgtggaa atgtaatgtg gtgtccggca
     5761 tcagccagcc ttgttcatcc attttcaggg tgatggcggc aatctcaagc agcgcatcgg
     5821 ttttggcgtt aaatccggct gtttcaacat cgatcacaac aggataaaaa ccacgaaaac
     5881 ggtcgcacag accggtaagt tgagcgttat cggacatctg attctcttaa tacggataaa
     5941 ttgcagcgcg cattatgaca aatattactt gtcgatgcag taaagatgcg ggcgcgatga
     6001 gttcacgccc ggcaggagat tagttgccca gaccgtgacc ggcgtctttc tcttcgatta
     6061 actcaatttt gtaaccgtcc ggatcttcca caaacgcgat aaccgtagta ccgcctttta
     6121 ccggacccgc ttcacgggtc acgttacccc cgttttgacg gattttttcg cacgcttcag
     6181 cggcgttatc tacgctaagc gcgatgtgac cataagcagt gccgagttcg tatttatcca
     6241 cgccccagtt gtaggtcagt tcaatcaccg cttcttcggt ttccgggccg tagccaacaa
     6301 acgccagtga gtatttgtat tccgggtttt cgctggtacg cagcagtttc atgcccagca
     6361 ctttggtata aaaatcgatg gagcgttgca gatcgccaac gcgcagcatg gtatgaagaa
     6421 gacgcatctt tttatcctca aaatggctaa cagattcaaa atgttgtttt agtatagcgg
     6481 cggctttacg ccgctcgcaa tgttggatta caacgtcggg taatcggtat agccttccgc
     6541 gccgccaccg tagaaacttt cggcacgctg tgggttaagc tcagctttgc gctgcaagcg
     6601 ggcgaccaga tccgggttcg caatccagtc acgaccaaat gccaccgcat caattaaccc
     6661 tttgccgatc agcgtttcag ctttttctac tgtgtatgca cctgcgccga taatcggacc
     6721 gtggaaacgg gcgcgtactt tttcgcggaa cgcatcagta tacggttcac cccccgccca
     6781 atctggttct gacatatgca gataagcaat gccgcgttta cccagttgtt caatcagata
     6841 cagtgcatcg gcttcttcat tcgggccgtt atccgtgttc tggaaagtac cgattggcga
     6901 aacgcgaatg ccaatgcgat cggcacccca ttcttcaatc ccggcatcga ccacttccag
     6961 taccagacgt gcgcgatttt ccacgctgcc gccgtactga tcggtacgat ggtttgaaga
     7021 aggagaaagg aactgatgca gcaaataacc gtgagcagag tggagctcta ccagatcaaa
     7081 accggcttca cgcgcgttag caatggcctg acggaaatca ttgacgatac ctggaatctc
     7141 ttccagttca agcgcacgcg gcatggatgt ttcaacacgg atcgcctgac cattttcatc
     7201 gcgcagagaa gtacgtgttc ccgcgctaag tgctgaaggc gctaccggtg cctgaccgcc
     7261 aggttgcagg ctggcgtgag aaatgcgtcc ggtgtgccac agctgcacgg ccatatgacc
     7321 attttcagca tgaacgccag cggtgatttt tttccatgcg gcaatttgct ccggactatg
     7381 gatgccaggc gcacctgcat atccttttgc ctgggcagaa atttgcgtgg cttcactaat
     7441 aatcaaaccg gcactggcac gttggcgata gtattccgcc atcaacgggg taggaatgtc
     7501 acccggttca atactgcgca gacgcgtcag cggtgccata aaaatacggt ttgccgccgt
     7561 gatcgcgccc actttcagtg gggaatacag tttttcagat gacataaagg ctcctggtcg
     7621 tctggtgata aaaaatgcct gctaaacggc aggcgtcgca ataatgtttt ttacatgggc
     7681 cagcgcgttt tccagtggct cgaaactgcg cgaaattttg gcctgcagat tcgcgccaag
     7741 ccacagtgcg taaagcactt gtgcctgttg tagcggttcg ccacaaaagg ttaaacaatg
     7801 gttctcacgg ccattttcca gcgcctgaga gagcagggcg atcacgccgc gagcaccttt
     7861 atccatcgcg ctgcgcatat cttctgacag atcgcacact tcggcagaga gttttactgt
     7921 caggcaacca ctgatggttc catgttggca aaactggttc agggtttgct ggtaataagc
     7981 cagtatgcgg tcgcggtagt taccttcgcc ggattgcagc aactcagtca gtcgctggtg
     8041 atatgcggcg taatgacgct caagcatggc aacgccaaac gcttctttag agcgaaagta
     8101 gtgatagaag gaccctttcg gcacttcagc ggtttttagt aattcgctta gccccatccc
     8161 ggtgaatcca cgttgcaggc aaagttgctc gcccgtcgcc aggagatgtt cgcgagtatc
     8221 atgttcggtg tgtttgttca tgcgttggag tgtagtagac cagtcggtct agtgcaagca
     8281 aagttgctac tttggacatt aacagcttca ataatggtaa taatcttatc tgaggagggc
     8341 gaagtggcgg agcaattaga gttctttccc gtccagagcc cgtgccgggg aatttgccag
     8401 tctgacgaac gcgggttttg tcgcggctgt tttcgtagtc gtgatgagcg ttttaactgg
     8461 aataaaatga gcgatggcga aaaacaagag gtgctgcgcc tttgtcggca gcgattaatg
     8521 cgcaaattac gcgcaaataa gccagcgtca tcagacgaac cgaagcaacc atcactcttt
     8581 tgaacctaat cttgcgtata ctcctctcat atctgataag aggaagcgat tatggttcag
     8641 cgtattacta ttgcgccgca aggcccggag ttttcccgtt ttgtgatggg ctactggcga
     8701 ttgatggact ggaatatgtc cgcccgccag ctagtcagtt ttattgaaga gcatctggat
     8761 ctcggcgtga ccaccgtgga ccatgctgat atttatggtg gctatcagtg cgaagcggcg
     8821 tttggcgagg cactgaaact ggcacctcac ctgcgtgaac ggatggaaat cgtcagtaaa
     8881 tgcggtatcg cgacgaccgc gcgtgaagaa aacgtcattg gtcattacat cactgaccgc
     8941 gatcacatca ttaagagcgc cgaacagtcg ctaattaatc tcgcgaccga tcatctggat
     9001 ttgctgttaa tccaccgacc agacccgtta atggatgccg atgaagtggc ggacgcgttc
     9061 aaacatctgc atcagagcgg caaagtgcgt cattttggcg tatcgaactt tacgcctgcg
     9121 caatttgccc tgttgcaatc acgtctgccg tttacccttg ccactaatca ggtggaaata
     9181 tccccggtgc atcagccgtt actgctggat ggcacgctcg accaactaca acaactgcgt
     9241 gttcgtccga tggcgtggtc ctgccttggt ggtggtcgtc tgtttaatga tgattatttc
     9301 cagccgctgc gtgatgaact ggctgtggtg gcagaggagt taaacgcggg ctcgattgaa
     9361 caggtggttt acgcctgggt attacgttta ccatcgcagc cgctgccaat tatcggttca
     9421 ggtaaaattg agcgcgtacg ggcagctgtc gaagcagaaa cactgaaaat gacccgtcaa
     9481 caatggtttc gtatccgtaa agcggcactg gggtacgacg taccgtaagc acttttagga
     9541 atagccgccg ttcaaaaatg tgtcactggt ttacacttat tcaggaatgc acaatgaacg
     9601 gaggtcctat gaaacgtttt agtctggcta ttctggcgct ggttgttgca accggcgcac
     9661 aagctgccag tgaaaaagtc gagatgaacc tcgtcacgtc gcaaggggta gggcagtcaa
     9721 ttggtagcgt caccattact gaaaccgata aaggtctgga gttttcgccc gatctgaaag
     9781 cattaccccc cggtgaacat ggcttccata ttcatgccaa aggaagctgc cagccagcca
     9841 ccaaagatgg caaagccagc gccgcggaat ccgcaggcgg gcatcttgat ccacaaaata
     9901 ccggtaaaca tgaagggcca gaaggtgccg ggcatttagg cgatctgcct gcactggtcg
     9961 tcaataatga cggcaaagct accgatgccg tcatcgcgcc tcgtctgaaa tcactggatg
    10021 aaatcaaaga caaagcgctg atggtccacg ttggcggcga taatatgtcc gatcaaccta
    10081 aaccgctggg cggtggcggt gaacgctatg cctgtggtgt aattaagtaa ttacgaggcc
    10141 agcgtacctt gcggtggtgc ttgctcaagt tgcgaaagcg agcagtacag ccgccagaca
    10201 attgccgcca gctcacgggc ggcaggttga tgatgacggg caaggctgtt gcaaatccgc
    10261 tgtaattctt caagtgtggc cgccagtgat ttttgctgaa cgccacgctc actcatcact
    10321 ccccgcaaca gtgaaataca gttatcccgt actcgcgata acggatcgga acgcgattcc
    10381 cagtcgcgca attgccagac aacatgagaa cagttcagca gcactacacc ccagcgtaat
    10441 aaccaacggc gagccagcgc atcctggctg ttactcaact gactgacgtg atgataagtg
    10501 agcgattcaa attcgctttc actcagtgtt ggatggcggc ttagctgatc gacaaaatcc
    10561 cggcgcagcg cgcgaatatg gcggcggctt ttacgagcat ccgatcctgg acgcagaatg
    10621 gcgaacgcta gccaagccag cgcaacgcca acgatttttg ccagattatc gttaagaaaa
    10681 tcagcaaaat catacaccgg gggattagtg acagcgataa acgaacccat aaaaacaatc
    10741 agttgccccc acagtgcggc aaatttgggc atctgcaatt taagaagctg cattgtcgcc
    10801 agcagtggaa agagaaacag taaaaattgc cacagatcgc taatctggac catcaggcca
    10861 aatttgacca caaagctgaa tagcgaaagt aacaccagcg tgcgcatcag cagtgacaac
    10921 gacttaaacg gtgctgcgac ggcggagtag agtacgcagc taattgctgc cagcgttaat
    10981 gcattggcac cggcatccca ttgcgatgca atactccatg cgccaatcat catcaacgta
    11041 caaaatgtac gcagcccgct ccacatagct tcggtgttgt cggtatgacg agccagagcg
    11101 gatgtccgtg cgagtctggc atgatcatct acaccgcttt gcagacgatg taattcctga
    11161 ctactttgca gataaaggcg gcaaaaataa cgtagtcgct gccagaaggc gacgtgccgg
    11221 tagtcggcga cgttggtcgg gcgtagcggg gcgataatac gtgcgacggt gtaagcatct
    11281 gtttgcgaac tggcgagcgc cgtcagcaat tgttcgagaa tttctcgcgt ggcacctggc
    11341 ggtgagggcc agttgagcaa catacgtcgc aggctggaga tgacactggt catacgtaat
    11401 tgctggtgga gtagcgcatt aaggcgcgcg ttttgctggc gaaaacgata gtggctccag
    11461 aaagcctgga tacgcagcaa attcatggtc agtatctgcc caatcacccc ctcatgtgct
    11521 gcacgaatgg catcgtttgt ttcaggctgc cagagtaaac tggcatgttc cagtaatcgg
    11581 gcgtgcatgt ttttcaatgc ggttaaaagg gctgtagcat cggaactgct aggcaggatc
    11641 atcatcatca tgccgccgca caaaataccg actatcacct cgcaaacgcg cgcctgagcg
    11701 atatcccaca gctggctggc ctcagtaata ttaaccatcg gaaaggcgat gatggcagcc
    11761 gtgtagcccg ccagttgaaa tgcatacgcg acgttattcg tgaagtgcgc acaggcccag
    11821 gtacaaaagc caagccacgc cgacatgctc aatagaaaaa accacggctc attgagcgta
    11881 tgcccggcaa gaagcagtgc cgcaatggct cccagtaaac tgccagcgat gcgtccgagg
    11941 cttttgctga tgacaccgcc aacggtggga aagctaacca ctgcagccga ggtcatcgcc
    12001 caatagggtt catccagatt caaataatag gcaaccgtca gcgccagaca catggcaatg
    12061 gtattgcgta acgcataacg ccattgcgcc agcgtggcct tgaaccaggg caaattgcgc
    12121 aaggaccatg acgatgcgtt cattattgac ctacggcaat gctacaggtg gtgcccgaca
    12181 ccagcgtgac atcgcctgga actttatcca gcgcaaagcg gacgggaacg cgttgggcga
    12241 gacgaaccca ggggacgtta ggtttcacat ccggaataag gcttgagtca ctttcaacgc
    12301 tttgatcata aatcgcccga ccgatactgg aaacgtgacc ccgtaacgtt ttgttatcac
    12361 tatagagggt aatttgtgct ggtgcacctt cgcggatatg gcgcaacttg gtttcttcaa
    12421 aataaccaat gacgtaaaac gagtggctgt cgacaagggc aaacagcggt ttcccggtgt
    12481 cagcgtaatc gccgatgcgg gtagtgaggt tagtcaccca accactcacc ggggcgcgaa
    12541 tttccgtttg cgccagttgc cattgtgtct gtttcagcgt ggcctgtgcg gcattaacac
    12601 tggcctgcat cgctttaaca ttgaggttag cggtatcgag ttcttcggca gaaataaaat
    12661 tttgtgagag atgacgacga cgattcgctt cgttattggc ttttgcgagg tcagattgcg
    12721 ctttcgccag ctgagcctgt gcgttcagct cggcgatctg aaagggcgtt ttatcgatcg
    12781 tgagcagaag atcccccgcg ttaaccagtt ggttgtcttt tatattcagc tcaacaatac
    12841 gtcccgacac ctgtggggtg atagaaacct tttctgcgcg tattttcccg tcgcgtgtcc
    12901 acggagattg catgtaataa ttccacagcc accatccggc aagaaccgca actatcgcta
    12961 caatgattgt tgagaaatac ttaattgttt taattgacat agttaccacg caatcagtat
    13021 tgccagagca aggcaaacgc aaatcgcaaa cagcgataaa tccattaaca agggatgcca
    13081 gatgtcaccg gcgtagatcc agccacgaag caggcggtgt acgacaagcc agatgacgaa
    13141 tccaaacgcg aaggctttga aaaacggagg aaaatagacg gacgcaccga acaccagatc
    13201 ttgtaagggc aatcctgttg cattgagcat aaacttcaca aagtagattc ctttacgacc
    13261 gaatatattg cgttagatta ataaatattc tttaagtgcg aaaaatttac gcgcaatttc
    13321 tgaaaaatat gtctgacggt aaccaaatgc agcaatacat ttgttttagc aatacaattg
    13381 ctgcacacta ttctaaaagc cgcataatat cttagcaagc taattataag gagatgaaat
    13441 tggaatcgcc actaggttct gatctggcac ggttggtgcg catatggcgt gctctgatag
    13501 accatcgcct gaaaccgctg gagttaacac aaacccattg ggttacgtta cacaatattc
    13561 atcagttacc tccagaccag tcgcaaattc aactggcaaa agcgattggc atcgagcagc
    13621 catcactggt ccgtactctg gaccaactgg aagaaaaagg gttaatttcg cgtcaaactt
    13681 gtgccagcga tcgtcgggct aaacgtatta aactgacgga aaaggcagag ccactgatca
    13741 gcgaaatgga agctgttatt aacaaaaccc gcgcggaaat attacatggc atctccgcag
    13801 aggaactgga gcaactgatt acgctcatcg caaaacttga gcataatatc attgagttac
    13861 aggccaaagg gtgaaatgaa gggggcgtgt ggtcagtaac ctgaccacac gtaaacttat
    13921 ttagcgcgga gaaacggtca cctgactgcc attgctggcc agtacgacac gttggcccgt
    13981 agagaaacga gtgttgcctt gtttctgtac caccatgatg gtattaccat cgtctttacg
    14041 aatttccagc tcgacaccct gcgttttgtt cattgcactc tgtacgccct gaccagctac
    14101 gccacctgca acagcgcctg ctgcagtagc cagagaacgc ccggttccgc caccaacagt
    14161 attccccagg aaaccaccaa gaacagcacc gccaattgca ccgataacgt tggaatcatc
    14221 accgccctga atctgtaccg gacgtacgtt aacgatggtg ccatagctga cattctgtac
    14281 ttgtttcgct tcagaagcgg tataaacatc ccctgacagg gtgtcgttat taacacaacc
    14341 gacaagagac agacctacca ttgaaacaac caatacgcgt ttaatcattg aaaaatctcc
    14401 tgttcaccat gaaacgccac gcaagcatcc ctcatggtca aagtatatgg catatgtgaa
    14461 tccatggtta catgttctac caaatatgag caaatcataa tgaaagtagg atcaaccaat
    14521 tataaacaaa acattcatga tgattattga tttgaagata atgttttact accaacggta
    14581 aaaatcggtc attccgcatg gtattcacga aaagtttatg tttgagtggt gcgcaaacgt
    14641 cagattgaaa aaggatgaac tatgaaatcg ggccgcttta ttggcgttat gtcaggcacc
    14701 agccttgatg gtgttgatgt tgtgttggcg acaatagatg aacaccgggt cgcacagctg
    14761 gcaagcttga gctggccgat cccggtgtct ctgaaacagg ctgtactgga tatttgccag
    14821 ggccagcagc ttacactttc gcagtttgga cagcttgata ctcaactcgg gcgacttttt
    14881 gctgatgcgg tcaatgcctt gcttaaggaa caaaacctgc aggcgagaga tatagttgcg
    14941 atcggctgtc acggtcaaac cgtctggcat gaaccgacgg gcgtggcacc acacacttta
    15001 cagattggcg ataacaatca aattgtggca cgcaccggaa ttacggttgt cggtgatttt
    15061 cgccgtcgcg atattgcctt gggaggacaa ggcgcaccgc tggtacctgc gttccatcat
    15121 gccttgctgg ctcactcaac cgagcgacga atggtgctca atattggcgg catcgccaat
    15181 ctgtcactgc tcattcctgg gcagccggtt ggcggctacg ataccggtcc tggtaacatg
    15241 ctgatggatg cctggatctg gcgtcaggcc ggtaaacctt acgataaaga tgccgagtgg
    15301 gcacgggcgg gtaaagttat tctcccactg ctgcaaaata tgctcagcga cccatatttc
    15361 tcgcaacctg caccgaaaag caccggacgc gaatacttta actacggctg gctggagcgc
    15421 catttacgcc attttccggg tgttgatccc cgtgatgttc aggccacact ggcagaactc
    15481 accgccgtga ccatttctga acaagttttg ttgagcggtg gctgcgaacg attgatggta
    15541 tgtggtggag gtagtcgtaa tccgctactc atggcgcgtc tggcggcatt actgccaggc
    15601 acagaagtca ccaccaccga tgccgttggc attagtggcg atgacatgga agcattggct
    15661 ttcgcctggc ttgcctggcg gacgctggcg ggattaccag gaaatctgcc ttccgtcact
    15721 ggcgcaagcc aggagacggt actgggggct attttccctg ctaacccgtg atatcagagt
    15781 taactgaatt tttcttaccg cgtctgccgt tacacttgga acgttaggga gggcgtattg
    15841 ccctccagac caggaaagtc ttcgggaaat gtctatgaaa aaactgttac tgatctgttt
    15901 gccggtgctt ctcaccggct gtagcgcgtt caatcaactg gttgagcaca tgcaaaccgc
    15961 tacgctggaa taccagtgtg atgaaaaacc gttgacggtc aaactgaata atccgcgcca
    16021 ggaagtcagt tttgtttacg ataatcaact actgcatctc aaacagggca tttcagcgtc
    16081 tggtgcacgt tacactgacg gaatctatgt tttctggtcg aaaggcgatg aagcgactgt
    16141 ctataaacgc gaccgcatcg tattgaataa ctgtcagtta caaaatccac agcgttgaga
    16201 tttttccagg ggcggcgcac aatagcgtca cccactgaca atccgtaaag aaaaccatgt
    16261 ctgataacga cgaattgcag caaatcgcgc atctgcgccg tgaatacacc aaaggcgggt
    16321 tacgccgtcg cgatcttccc gccgatccat taaccctttt tgaacgttgg ctctctcagg
    16381 cgtgtgaagc caaactggca gaccctaccg cgatggtggt cgctaccgtg gatgaacatg
    16441 ctcagcctta tcaacgcatc gttttactca aacattacga cgaaaaaggc atggtgtttt
    16501 acaccaacct cggtagccgt aaagcacatc aaattgaaaa taatccgcgc gttagcctgc
    16561 tgttcccgtg gcataccctt gaacgccagg tgatggtgat cggtaaagca gaacgacttt
    16621 cgactctcga agtgatgaaa tattttcata gccgcccgcg tgatagccag attggtgcat
    16681 gggtttcgaa gcagtccagt cgcatttctg cccgcggtat ccttgaaagt aaattcctgg
    16741 agctgaagca gaagtttcaa cagggcgaag tgccattgcc gagcttttgg ggcggttttc
    16801 gcgtcagcct tgaacagatt gagttctggc agggcggtga gcatcgcctg catgaccgct
    16861 ttttgtacca gcgagaaaat gatgcgtgga agattgatcg tcttgcaccc tgaaaagatg
    16921 caaaaatctt gctttaatcg ctggtactcc tgattctggc actttattct atgtctcttt
    16981 cgcatctggc gaaaagtcgt gtaccggcaa aggtgcagtc gttatataca tggagatttt
    17041 gatggcaagc agtaacttga ttaaacaatt gcaagagcgg gggctggtag cccaggtgac
    17101 ggacgaggaa gcgttagcag agcgactggc gcaaggcccg atcgcgctct attgcggctt
    17161 cgatcctacc gctgacagct tgcatttggg gcatcttgtt ccattgttat gcctgaaacg
    17221 cttccagcag gcgggccaca agccggttgc gctggtaggc ggcgcgacgg gtctgattgg
    17281 cgacccgagc ttcaaagctg ccgagcgtaa gctgaacacc gaagaaactg ttcaggagtg
    17341 ggtggacaaa atccgtaagc aggttgcccc gttcctcgat ttcgactgtg gagaaaactc
    17401 tgctatcgcg gcgaacaact atgactggtt cggcaatatg aatgtgctga ccttcctgcg
    17461 cgatattggc aaacacttct ccgttaacca gatgatcaac aaagaagcgg ttaagcagcg
    17521 tctcaaccgt gaagatcagg ggatttcgtt cactgagttt tcctacaacc tgttgcaggg
    17581 ttatgacttc gcctgtctga acaaacagta cggtgtggtg ctgcaaattg gtggttctga
    17641 ccagtggggt aacatcactt ctggtatcga cctgacccgt cgtctgcatc agaatcaggt
    17701 gtttggcctg accgttccgc tgatcactaa agcagatggc accaaatttg gtaaaactga
    17761 aggcggcgca gtctggttgg atccgaagaa aaccagcccg tacaaattct accagttctg
    17821 gatcaacact gcggatgccg acgtttaccg cttcctgaag ttcttcacct ttatgagcat
    17881 tgaagagatc aacgccctgg aagaagaaga taaaaacagc ggtaaagcac cgcgcgccca
    17941 gtatgtactg gcggagcagg tgactcgtct ggttcacggt gaagaaggtt tacaggcggc
    18001 aaaacgtatt accgaatgcc tgttcagcgg ttctttgagt gcgctgagtg aagcggactt
    18061 cgaacagctg gcgcaggacg gcgtaccgat ggttgagatg gaaaagggcg cagacctgat
    18121 gcaggcgctg gtcgattctg aactgcaacc ttcccgtggt caggcgcgta aaactatcgc
    18181 ctccaatgcc atcaccatta acggtgaaaa acagtccgat cctgaatact tctttaaaga
    18241 agaagatcgt ctgtttggtc gttttacctt actgcgtcgc ggcaaaaaga attactgtct
    18301 gatttgctgg aaataatgca ttaagtggaa agggggagtg agaaatcact ccccctggtt
    18361 tttatacagg gaacatgatg aaaaatattc tcgctatcca gtctcacgtt gtttatggtc
    18421 atgcgggtaa cagtgcggca gagtttccta tgcgccgcct gggcgcgaac gtctggccgc
    18481 tgaacaccgt tcaattttct aatcacaccc aatacggcaa atggactggc tgcgtgatgc
    18541 cgcccagcca tttaaccgaa attgtgcaag gcattgccgc cattgataaa ttacacacct
    18601 gtgatgccgt gttaagtggc tatctgggat cggcggagca gggtgaacat atcctcggta
    18661 tcgtccgtca ggtgaaagcc gcgaatccgc aggcgaaata tttttgcgat ccggtaatgg
    18721 gtcatccgga aaaaggctgt atcgttgcac cgggtgtcgc agagtttcat gtgcggcacg
    18781 gtttgcctgc cagcgatatc attgcgccga atctggttga gctggaaata ctctgtgagc
    18841 atgcggtaaa taacgtcgaa gaagcggttc tggcagcgcg cgaactcatt gcgcaagggc
    18901 cacaaattgt gttggttaaa cacctggcgc gagctggcta cagccgtgac cgttttgaaa
    18961 tgctgctggt caccgccgat gaagcctggc atatcagccg tccgctggtg gattttggta
    19021 tgcgccagcc ggtaggtgtt ggtgatgtga cgagcggttt actgctggtg aaactgcttc
    19081 agggggcaac gctgcaggag gcgctggaac atgtgaccgc tgcagtctac gaaatcatgg
    19141 tgaccaccaa agcaatgcag gaatatgagc tgcaagtggt ggctgctcag gatcgtattg
    19201 ccaaaccaga acattacttc agcgcaacaa agctctgaaa tatctcattt cggcccacaa
    19261 cgacctgtgg gccgatggat attactttaa gccttccgct gacagcgcgt cttgtacttc
    19321 cggacgttca gccatacgtt gcataaatgc tgcaatgtgc tctaaccctt ccagattcag
    19381 tttcaccgcg tatgcccagc gcagaaccgt aaacagatag gcatcagcaa ttgtaaatct
    19441 ttgcccgcag atccagtgct catccttcag tgcctcgttc acatattgca gcttcttctc
    19501 cagctgcgcg cgaactgtcg gtttgtactc ttccggtgta tcagggcgaa acagaggtgt
    19561 gaaaccttta tgcagctcgg tggcgatgta attcagccat tcgatggttt tatagcggga
    19621 aatactgttt accggtgcca gcaactggcg gtcggggacg ctgtcggcaa gatactgcat
    19681 aatcgctacg ccttccgtca gcaaagtacc gtcatccagc agcaatgcag gcacctgccc
    19741 cttagggtta acgacaaagt aatcgtcacc gttttcgaga cgttttttca ttaaatccac
    19801 actgacgagg gtaaaatcct ttccgctctc acgcagggtg atatgggaag cgagagagca
    19861 ggcacccggt ttgtagaaca atttcatcgg taactccttt tgtctgtggt ttaaagtatg
    19921 gtagtgcgtt gctgaacaaa aaaaaagccg ctaacgaaaa gttagcggct tgtaaaagag
    19981 tttccctgaa ttttacgcta cagcagcttt cgccgctttg tctgcagcgt catcctgcgt
    20041 catacggtgc agtttaggcg cggtcagcag catcagtact gcaataatgg cagtagcgac
    20101 accaatctgc aagaatacgc gaccataaac ttccagtgac atcagcgggt cggtaacgtt
    20161 atccggcaca gccatcatac ccgcaacata accaccaatc aggtttgcac cggcagtggt
    20221 caggaaccag ctacccataa tgaagcccat cagacgctgc ggaacgagtt gtgcaaccat
    20281 tgccagaccc agaccagaga tcatcagttc cccgatgctc tgcaggccat agcttgcgac
    20341 cagccagctt acagacacga taccagcgtc agacgcgaat ttcgcaccca gcggcagaat
    20401 caggaacgca ccagaacaca tcaccatgcc gattgcaaac ttggttggca tcggcagggt
    20461 atcgcccatc ttgttataga tagcggccag aatcggacta ccgatgatga tccagaacgg
    20521 gttcagtgcc tgatactgct caggttctac ggccagaccc agaatggagt gctcaacgtt
    20581 acgaatcgca aagaagttca gtgacgttgg catctggctg tacagcacga agaagataat
    20641 ggcttcgagc atcaggatga aggcaacgat cattttacga cgcgcagcac ctttcatcgc
    20701 gaaggcttct ttaccgaaga taaccacgat accgaaggca acaacgccca gcgccatacg
    20761 cgcaacttcc tgattgtgca gcagccaggt ggcgatagcg atcagtgcca caacaccaat
    20821 aatggtcagc agcaggttac ggtagttgat aggctcgaag tctggttttg aaccgtactg
    20881 tttaacccag cgttggcaga aggcgaagtt aacgatagtg atcagcaggc ctacaacgct
    20941 caacgcaaac gcaacactcc agccgtattt cgcggccagc cacggcgtag caatcataga
    21001 gaagaaagag ccgatgttga cggacatgta gtacatggtg aatgcaccgt ccagacgcgg
    21061 gtcgtttttc tcatagcatg tagaaagcag agaagacggg ttagctttaa acaggccgtt
    21121 accgaccgca atagccgcca tacccatata aacgataccg gcgtcgtgac cagaccaggc
    21181 aaccagagca taaccaatcg ccagcacaat agcgccgagc ataattacgc gtttagtacc
    21241 cagtaccttg tcacctaacc agccgccgat agcgaccaga ccataaacca gggcactaaa
    21301 ggaagagaaa agggtgattg aatccgcttc agacataccc agttgtttaa ccaggtaaac
    21361 agccataatt ccttgtaggc cgtaataacc aaaacgttcc cataactcaa tcgagaagat
    21421 gagatagaac gccttcggtt gtttgaaagc gttcaaactg acgctttcag ttggtttttg
    21481 gtttgcagtg gacactttta cctctttttt tacatcccat attaacgggg gtgctttctc
    21541 gcgttatgtc taaaaaacat acgcgcaatt gttatagtgg gaggggaaac ggcaggtaat
    21601 gttcactatc ttgcactttc agacaagtcc tttgtaataa tctgttacat ataactgagg
    21661 cggggaattc aaccattccg ggaaatgtta tccagcgtta aatttttcaa aaaaacgtaa
    21721 ctgcagattt tttcactgca ctgagagggg ggtgatggct taacggcgat atgttctgct
    21781 atttagtgtt tttgctagtg agatagtcca gtttctgaaa aatagccagt gtaatgtttt
    21841 gtaggtcaat atttgttgcg tgtgtaaatt taaatgattt taacctgatt cttgcaatgt
    21901 ttttacattg cgacagcatc gtgatcttga tcacgaatga atagaaaatt tcgctattaa
    21961 aaaaacaatt aacctgcatt tttggttatc tgcaaacgga ttacctgatg ataacgctaa
    22021 gcaatggcat cattcacgtt aaaaagatgt tttaaaagag gataaagaaa ggttatcaat
    22081 ggggtaatcg gtgttacccc ttttcttcag atgtcaactt tctctttgta ttcacaaaga
    22141 tcttcaataa tacaagagcc acagcggggc ttgcgggcaa tgcaggtata acgcccgtgc
    22201 aggatcaacc aatggtggca gtcgacttta aactctgccg gaaccacttt cagtagcttt
    22261 tcttctacct gttcgacgtt tttccccggc gcaaattgag tacgattaca aacgcggaaa
    22321 atgtgcgtgt cgacagcaat agtcggccag ccgaatgcag tgtttaatac gacgttggct
    22381 gttttacgac ctacgccggg cagggcttca agcgcagcac gatcttccgg aacctcgcca
    22441 ttatgctgct ccagcaagat acggcaggtt ttgatgatat tttctgcttt gctgttataa
    22501 agcccaatcg ttttgatata ggttttcacc ccttcaacgc ccagttcaag catcgctgca
    22561 ggcgtattcg ccaccgggta gagtttcgcc gtcgccttat taacactgac atcggtcgcc
    22621 tgagcggaaa gcagtacggc aatcagcaat tcaaaaggcg aactgaaatt aagctcggtg
    22681 gtgggatgag gattgttctc acgcaggcga gtgaggatct ccaggcgttt tgctttgttc
    22741 atcagacatt ccctgtttca ccgtttggca atgcacgttc ggcagttgct tcagcacggc
    22801 gctttttcat tctttcatca atcaggtatt ttcctgccag catcagtccc aggccaataa
    22861 atgcacctgg aggcagcatc gccagcagga aaggggagtc ggtgtggaaa atttccacgc
    22921 gtaatacttt tgcccagcta cctaacagcg catctgcacc gtcaaacaat gtgccattgc
    22981 cgataatttc gcgtagtgaa cccagcacga acatggcgcg ggttgcgccc ataccaattg
    23041 aaaagccgtc cagtgccgaa agcgccggac cttttttggc ggcgaaggct tcagcgcggc
    23101 ccacaacgat acagttagtg acaatcagcg gaataaaaat ccctaatgat tgatacaggc
    23161 caaaggcgta ggcgttgatc agcatctgta cagcgctgac caccgaggcg atgatcatca
    23221 cgtaaatggg aatgcggatc tcggctggcg tccagtgacg cagcgtcgaa atggtcaggt
    23281 tggtcagcgt cagtaccagc gtagtcgcaa gtcctaaacc cagagcgtta gtggcagtgg
    23341 acgtgaccgc caacagagga caaaggccga gcaactggac cagcgcagag ttgtttttcc
    23401 acaccccctg aacaataacg tctttaattt cgctcacgct ttattctcca caggcaggaa
    23461 gttgagaaag ttgtgccggt aacgtctgag cgtacaatcc ggcgcgtttt accgcattaa
    23521 ccaccgcgcg gggagtaatc gtcgcgccgg tgaactggtc gaaatcacca ccatctttct
    23581 tcaccgccca gtgcgcatca tccgcaccac tgattttttt acccgcaaaa tgggtgatcc
    23641 agtcagaaag gcgcagttcg attttatcgc caagccctgg cgtttcgtgg tgctctgtca
    23701 cgcgcgtgcc aagtaccgtg ccgttaaaat cggctcccac cagcagctga atcgcaccgg
    23761 aatagccatc tggcgcggtt gcttccagaa cggcggctac cggtttgtca tcctgtttgg
    23821 cgatgtaaac ccgatgctca cctttaccta actctggcgc agttaccaga tagcaactct
    23881 gtgccagcgc attgttatag cgttcggctg gcagcacctg atcaaataat gcctcttgtt
    23941 gcagactggc ctgttcagca atcgtcgttt tggtcatctg gttgatggcc gcagttaacc
    24001 ctgttgaacc cgctgcaaat agcgctaacg taatgccgtg ttttcggata gttttcagca
    24061 tggtttaccc tttgcgatgg ccgtagacgc gcggacgcgt gtagtaatcg atcagaggaa
    24121 ccgtgatgtt cgccagcagg acggcaaaag ccacgccgtc aggatagccg ccgaaactgc
    24181 ggatcaacca gactaataag cccgccagcg cgccgaaaat aagacgacca cgattggtcg
    24241 tagaagcggt aaccgggtca gtcaaaataa agaatgcgcc gagcatggtc gcaccagaca
    24301 gcagatgaat ttgcggtgct gccagtgttt ctggtgagaa caaccagccc aacgttgcgc
    24361 ataacgccag cgttactaag aagctgaggg gaatatgcca gcgaatcgct ttctgccata
    24421 gcaaccacag gccgccagcc agccaggcga gatttaccca ttgccaacca gcgcccgcca
    24481 gaataccgct gtagatcgga tattgcataa tctgttcaac cgaatgaccg gcacggacag
    24541 aggttttaaa tgtatccagc ggtgtcgcct gactaatgcc atcaatacct aagcgtagtg
    24601 tgttcatatc accaccactg gcggtatgtc cgctgaaaat aacctgtatg gcgtcgataa
    24661 aaccagggat gttgaccgca atttcatgtg gcggtaacca gctggtcatc tgcaccggga
    24721 aggagatcag taagaccaca taaccaatca ttgccggatt aaacgggttt tgccccagac
    24781 cgccatacaa ctgtttagcg atgatcaccg caaacaccgt acccagcacg accatccacc
    24841 atggcgcgag ggggggaata cttaccgcca gcaataagcc tgtcagcaat gctgagttat
    24901 ctttcaacgt tgcggctacc gactgcttgc gtagtttgag tacgagagct tcggctaaca
    24961 gagcactaac cgatgccaac aggatctgaa cgagagtacc ccaaccaaaa aaccacagtt
    25021 gcgctgcgat tcctggcacg gctgcgagca acaccaacag cataatgcgc gatgtctggc
    25081 gctggttatg ggtataaggg gagctagcta ttctgaatac catttagtcc tcgtttacaa
    25141 ccttctgctg ggcggctttt ttggcctgag cgcgggcaat agccgcggca actgccgctt
    25201 tgcgcggatc aacctgttct tctggttctg cgttagtctg ttgctgttcc agcttgcgcg
    25261 ctttggcacg ggcaatagcg gcttcgacgg cggctttgcg cggatcaac
//
LOCUS       DAZFQD010000067        24898 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.067, whole genome shotgun sequence.
ACCESSION   DAZFQD010000067 DAZFQD010000000
VERSION     DAZFQD010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24898
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.067"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            119..1093
                     /gene="hemB"
                     /locus_tag="ACHKTW_002782"
     CDS             119..1093
                     /gene="hemB"
                     /locus_tag="ACHKTW_002782"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HFO6463780.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
     gene            complement(1199..2050)
                     /gene="tauD"
                     /locus_tag="ACHKTW_002783"
     CDS             complement(1199..2050)
                     /gene="tauD"
                     /locus_tag="ACHKTW_002783"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HFO6463781.1"
                     /translation="MSERLSITPLGPYIGAQISGADLTRPLSDNQFEQLYHAVLRHQV
                     VFLRDQTITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETPPAGAILAAKELPSTGGDTLWTSGIAAYEALSVPFRQLLSGLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     KPFYRAG"
     gene            complement(2047..2874)
                     /gene="tauC"
                     /locus_tag="ACHKTW_002784"
     CDS             complement(2047..2874)
                     /gene="tauC"
                     /locus_tag="ACHKTW_002784"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HFO6463782.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPLQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVVIGIPVG
                     IAMGLSPTVRGILDPVIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            complement(2871..3638)
                     /gene="tauB"
                     /locus_tag="ACHKTW_002785"
     CDS             complement(2871..3638)
                     /gene="tauB"
                     /locus_tag="ACHKTW_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HFO6463783.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQMLKKVGLEGAEKRYIWQLSGGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSSGPGRVLERLPLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            complement(3651..4613)
                     /gene="tauA"
                     /locus_tag="ACHKTW_002786"
     CDS             complement(3651..4613)
                     /gene="tauA"
                     /locus_tag="ACHKTW_002786"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO6463784.1"
                     /translation="MAISSRNTLLAALAFIAFQAQAVNVTVAYQTSAEPAKVAQADNT
                     FAKESGATVDWRKFDSGASIVRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKL
                     GNSEALVVKKTISKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGQVEIVNLQPPA
                     IIAAWQRGDIDGAYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHP
                     EVVKAFAKSAIDAQQPYIANPDAWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQ
                     QQTAELTGPVNKAIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
     gene            5229..5900
                     /locus_tag="ACHKTW_002787"
     CDS             5229..5900
                     /locus_tag="ACHKTW_002787"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HFO6463785.1"
                     /translation="MDKVLDSALLSSANKRKGILAIGAHPDDIELGCGASLARLAQKG
                     IYIAAVVMTTGNSGTDGIIDRHEESRNALKILGCHQTIHLNFADTRAHLQLNDMISAL
                     EDIIKNQIPSDVEIMRVYTMHDADRHQDHLAVYQASMVACRTIPQILGYETPSTWLSF
                     MPQVFESVKEEYFTVKLAALKKHKSQERRDYMRHDRLRAVAQFRGQQVNSDLGEGFVI
                     HKMIL"
     gene            5910..7106
                     /gene="yaiP"
                     /locus_tag="ACHKTW_002788"
     CDS             5910..7106
                     /gene="yaiP"
                     /locus_tag="ACHKTW_002788"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFO6463786.1"
                     /translation="MKTWIFICMSIAMLLWFLSTLRRKPSQKKGCIDAIIPAYNEGPC
                     LAQSLDNLLRNPYFCRVICVNDGSTDNTEAVMAEVKRKWGDRFVAVTQKNTGKGGALM
                     NGLNYATCDQVFLSDADTYVPPDQDGMGYMLAEIERGADAVGGIPSTALKGAGLLPHI
                     RATVKLPMIVMKRTLQQLLGGAPFIISGACGMFRTDVLRKFGFSDRTKVEDLDLTWTL
                     VANGYRIRQANRCIVYPQECNSPREEWRRWRRWIVGYAVCMRLHKRLLFSRFGIFSIF
                     PMLLVVIYGVGIYLTTWFNEFITTGPHGVVLAMFPLIWIGVVCVIGAFSAWFHRCWLL
                     VPLAPLSVVYVLLAYAIWIIYGLIAFFTGREPQRDKPTRYSALVEASTAYSQPSVTGT
                     EKLSEA"
     gene            7084..7665
                     /locus_tag="ACHKTW_002789"
     CDS             7084..7665
                     /locus_tag="ACHKTW_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001308433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFO6463787.1"
                     /translation="MKNYLKLNVKEKNIQIADQVIIDETAGEVVIGANTRICHGAVIQ
                     GPVVIGANCLIGNYAFIRPGTIISNGVKIGFATEIKNAVIESEATIGPQCFIADSVVA
                     NQAYLGAQVRTSNHRLDEQPVSVRTPEGIIATGCDKLGCYIGQRSRLGVQVIILPGRI
                     ISPNTQLGPRVIVERNLPAGTYSLRQELIRTGD"
     gene            7667..8440
                     /gene="yaiO"
                     /locus_tag="ACHKTW_002790"
     CDS             7667..8440
                     /gene="yaiO"
                     /locus_tag="ACHKTW_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiO family outer membrane beta-barrel protein"
                     /protein_id="HFO6463788.1"
                     /translation="MIKRTLLAAAIFSALPAYAGLTSITAGYDFTDYSGDHGNRNLAY
                     AELVAKVENATLLFNLSQGRRDYETEHFNATRGQGAVWYKWNNWLTTRTGIAFADNTP
                     VFARQDFRQDINLALLPKTLFTTGYRYTKYYDDVEVDAWQGGVSLYTGPVITSYRYTH
                     YDSSDAGGSYSNMISVRLNDPRGTGYTQLWLSRGTGAYTYDWTPETRYGSMKSVSLQR
                     IQPLTEQLNLGLTAGKVWYDTPTDDYNGLQLAAHLTWKF"
     gene            8625..8900
                     /gene="frmR"
                     /locus_tag="ACHKTW_002791"
     CDS             8625..8900
                     /gene="frmR"
                     /locus_tag="ACHKTW_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde-responsive transcriptional
                     repressor FrmR"
                     /protein_id="HFO6463789.1"
                     /translation="MPSTPEEKKKVLTRVRRIRGQIDALERSLEGDAECRAILQQIAA
                     VRGAANGLMAEVLESHIRETFDRNDCYSREVSQSVDDTIELVRAYLK"
     gene            8935..10044
                     /gene="frmA"
                     /locus_tag="ACHKTW_002792"
     CDS             8935..10044
                     /gene="frmA"
                     /locus_tag="ACHKTW_002792"
                     /EC_number="1.1.1.284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase"
                     /protein_id="HFO6463790.1"
                     /translation="MKSRAAVAFAPGKPLEIVEIDVAPPKKGEVLIKVTHTGVCHTDA
                     FTLSGDDPEGVFPVVLGHEGAGVVVEVGEGVTSVKPGDHVIPLYTAECGECEFCRSGK
                     TNLCVAVRETQGKGLMPDGTTRFSYNGQPLYHYMGCSTFSEYTVVAEVSLAKINPEAN
                     HEHVCLLGCGVTTGIGAVHNTAKVQPGDSVAVFGLGAIGLAVVQGARQAKAGRIIAID
                     TNPKKFDLARRFGATDCINPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRAALES
                     AHRGWGQSVIIGVAGSGQEISTRPFQLVTGRVWKGSAFGGVKGRSQLPGMVEDAMKGD
                     IDLEPFVTHTMSLDEINDAFDLMHEGKSIRTVIRY"
     gene            10137..10970
                     /gene="fghA"
                     /locus_tag="ACHKTW_002793"
     CDS             10137..10970
                     /gene="fghA"
                     /locus_tag="ACHKTW_002793"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018738 - S-formylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0046294 - formaldehyde catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HFO6463791.1"
                     /translation="MELIEKHASFGGWQNVYRHYSQSLKCEMNVGVYLPPKAANEKLP
                     VLYWLSGLTCNEQNFITKSGMQRYAAEHNIIVVAPDTSPRGSHVADADRYDLGQGAGF
                     YLNATQAPWNEHYKMYDYIRNELPDLVMQHFPATTRKSISGHSMGGLGALVLALRNPD
                     EYVSVSAFSPIVSPSQVPWGQQAFAAYLGENKDAWLNYDPVSLISQGQRVAEIMVDQG
                     LSDDFYAEQLRTPNLEKICQEMNIKTLIRYHEGYDHSYYFVSSFIGEHIAYHANKLNM
                     R"
     gene            complement(11095..11634)
                     /gene="yaiL"
                     /locus_tag="ACHKTW_002794"
     CDS             complement(11095..11634)
                     /gene="yaiL"
                     /locus_tag="ACHKTW_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2058 domain-containing protein"
                     /protein_id="HFO6463792.1"
                     /translation="MAKLTLQEQLLKAGLVTSKKAAKVERTAKKSRVQAREARAAVEE
                     NKKAQLERDKQLSEQQKQAALAKEYKAQVKQLIEMNRITIANGDIGFNFTDGNLIKKI
                     FVDKLTQAQLINGRLAIARLLVDNNSEGEYAIIPASVADKIAQRDASSIVLHSALSAE
                     EQDEDDPYADFKVPDDLMW"
     gene            complement(11736..12947)
                     /gene="mhpT"
                     /locus_tag="ACHKTW_002795"
     CDS             complement(11736..12947)
                     /gene="mhpT"
                     /locus_tag="ACHKTW_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-(3-hydroxy-phenyl)propionate transporter MhpT"
                     /protein_id="HFO6463793.1"
                     /translation="MSTRTPSSSSSRLMLTIGLCFLVALMEGLDLQAAGIAAGGIAQA
                     FALDKMQMGWIFSAGILGLLPGALVGGMLADRYGRKSILIGSVALFGLFSLATAIAWD
                     FPSLVFARLMTGVGLGAALPNLIALTSEAAGPRFRGTAVSLMYCGVPIGAALAATLGF
                     AGANLAWQTVFWVGGVVPLILVPLLMRWLPESAVFAGEKQAAPPLRALFAPETATATL
                     LLWLCYFFTLLVVYMLINWLPLLLVEQGFQPSQAAGVMFALQMGAASGTLMLGALMDK
                     LRPVTMSLLIYSGMLASLLALGTVSSFNGMLLAGFVAGLFATGGQSVLYALAPLFYSS
                     QIRATGVGTAVAVGRLGAMSGPLLAGKMLALGTGTVGVMAASAPGILVAGLAVFILMS
                     RRSRMQPCADA"
     gene            complement(13222..14235)
                     /gene="mhpE"
                     /locus_tag="ACHKTW_002796"
     CDS             complement(13222..14235)
                     /gene="mhpE"
                     /locus_tag="ACHKTW_002796"
                     /EC_number="4.1.3.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxovalerate aldolase"
                     /protein_id="HFO6463794.1"
                     /translation="MNGKKLYISDVTLRDGMHAIRHQYSLENVRQIAKALDDARVDSI
                     EVAHGDGLQGSSFNYGFGAHSDLEWIEAAADVVKHAKIATLLLPGIGTIHDLKNAWQA
                     GARVVRVATHCTEADVSAQHIQYARELGMDTVGFLMMSHMTTPENLAKQAKLMEGYGA
                     TCIYVVDSGGAMNMSDIRDRFRALKAELKPETQTGMHAHHNLSLGVANSIAAVEEGCD
                     RIDASLAGMGAGAGNAPLEVFIAAADKLGWQHGTDLYALMDAADDLVRPLQDRPVRVD
                     RETLALGYAGVYSSFLRHCETAAARYGLSAVDILVELGKRRMVGGQEDMIVDVALDLR
                     NNK"
     gene            complement(14232..15182)
                     /gene="mhpF"
                     /locus_tag="ACHKTW_002797"
     CDS             complement(14232..15182)
                     /gene="mhpF"
                     /locus_tag="ACHKTW_002797"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase"
                     /protein_id="HFO6463795.1"
                     /translation="MSKRKVAIIGSGNIGTDLMIKILRHGQHLEMAVMVGIDPQSDGL
                     ARARRMGVATTHEGVIGLMNMPEFADIDIVFDATSAGAHVKNDAALREAKPDIRLIDL
                     TPAAIGPYCVPVVNLEANVDQLNVNMVTCGGQATIPMVAAVSRVARVHYAEIIASIAS
                     KSAGPGTRANIDEFTETTSRAIEVVGGAAKGKAIIVLNPAEPPLMMRDTVYVLSDEAS
                     QDDIEASINEMAEAVQAYVPGYRLKQRVQFEVIPQDKPVNLPGVGQFSGLKTAVWLEV
                     EGAAHYLPAYAGNLDIMTSSALATAEKMAQSLARKAGEAA"
     gene            complement(15179..15988)
                     /gene="mhpD"
                     /locus_tag="ACHKTW_002798"
     CDS             complement(15179..15988)
                     /gene="mhpD"
                     /locus_tag="ACHKTW_002798"
                     /EC_number="4.2.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-4-pentenoate hydratase"
                     /protein_id="HFO6463796.1"
                     /translation="MTKHTLEQLAADLRRAAEQGEAIAPLRDLIGIDNAEAAYAIQHI
                     NVQYDVAQGRRVVGRKVGLTHPKVQQQLGVDQPDFGTLFADMCYGDNEIIPFSRVLQA
                     RIEAEIALVLNRDLPATDITFDELYNAIEWVLPALEVVGSRIRDWSIQFVDTVADNAS
                     CGVYVIGGPAQRPAGLDLKNCAMKMTRNNEEVSSGRGSECLGHPLNAAVWLARKMASL
                     GEPLRAGDIILTGALGPMVAVNAGDRFEAHIEGIGSVAATFSSAAPKGSLS"
     gene            complement(15998..16864)
                     /gene="mhpC"
                     /locus_tag="ACHKTW_002799"
     CDS             complement(15998..16864)
                     /gene="mhpC"
                     /locus_tag="ACHKTW_002799"
                     /EC_number="3.7.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414883.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-6-oxonona-2,4-dienedioate hydrolase"
                     /protein_id="HFO6463797.1"
                     /translation="MSYQPQTEAATSRFLNVEEAGKTLRIHFNDCGQGDETVVLLHGS
                     GPGATGWANFSRNIDPLVEAGYRVILLDCPGWGKSDSIVNSGSRSDLNARILKSVVDQ
                     LDIAKIHLLGNSMGGHSSMAFTLNWPERVGKLVLMGGGTGGMSLFTPMPTEGIKRLNQ
                     LYRQPTIENLKLMMDIFVFDTSDLTDALFEARLNNMLSRRDHLENFVKSLEANPKQFP
                     DFGPRLAEIKAQTLIVWGRNDRFVPMDAGLRLLSGIAGSELHIFRDCGHWAQWEHADA
                     FNQLVLNFLARP"
     gene            complement(16882..17826)
                     /gene="mhpB"
                     /locus_tag="ACHKTW_002800"
     CDS             complement(16882..17826)
                     /gene="mhpB"
                     /locus_tag="ACHKTW_002800"
                     /EC_number="1.13.11.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydroxyphenylpropionate/2,
                     3-dihydroxicinnamic acid 1,2-dioxygenase"
                     /protein_id="HFO6463798.1"
                     /translation="MHAYLHCLSHSPLVGYVDPAQEVLDEVNGVIASARERIAAFSPE
                     LVVLFAPDHYNGFFYDVMPPFCLGVGATAIGDFGSAAGELPVPVELAEACAHAVMKSG
                     IDLAVSYCMQVDHGFSQPLEFLLGGLDKVPVLPVFINGVATPLPGFQRTRMLGEAIGR
                     FTSTLNKRVLFLGSGGLSHQPPVPELAKADAHMRDRLLGSGKDLPASERELRQQRVIS
                     AAVKFVEDQRTLHPLNPIWDNQFMTLLEQGRIQELDAVSNEELSAIAGKSTHEIKTWV
                     AAFAAISAFGNWRSEGRYYRPIPEWIAGFGSLSARTEN"
     gene            complement(17828..19492)
                     /gene="mhpA"
                     /locus_tag="ACHKTW_002801"
     CDS             complement(17828..19492)
                     /gene="mhpA"
                     /locus_tag="ACHKTW_002801"
                     /EC_number="1.14.13.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008688 - 3-(3-hydroxyphenyl)propionate
                     hydroxylase activity [Evidence IEA];
                     GO_process: GO:0019622 - 3-(3-hydroxy)phenylpropionate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid
                     hydroxylase"
                     /protein_id="HFO6463799.1"
                     /translation="MAIQHPDIQPAVNHSVQVAISGAGPVGLMMANYLGQMGIDVLVV
                     EKLDKLIDYPRAIGIDDEALRTMQSVGLVENVLPHTTPWHAMRFLTPKGRCFADIQPM
                     TDEFGWPRRNAFIQPQVDAVMLEGLSRFPNVRCLFSRELEAFSQQDDEVTLHLKTAEG
                     QRETIKAQWLVACDGGASFVRRTLNVPFEGKTAPNQWIVVDIANDPLSTPHIYLCCDP
                     VRPYVSAALPHAVRRFEFMVMPGETEEQLREPQNMRKLLSKVLPNPDNVELIRQRVYT
                     HNARLAQRFRIDRVLLAGDAAHIMPVWQGQGYNSGMRDAFNLAWKLALVIQGKARDAL
                     LNTYQQERRDHAKAMIDLSVTAGNVLAPPKRWQGTLRDGVSWLLNYLPPVKRYFLEMR
                     FKPMPQYYGGALVREGEAKHSPVGKMFIQPKVTLENGDVTLLDNAIGANFAVIGWGCN
                     PLWGMSDEQIQQWRALGTRFIQVVPEVQIHTAQDNHDGVLRVGDTQGRLRSWFAQHNA
                     SLVVMRPDRFVAATAIPQTLGKTLNKLASVMTLTRPDADVSVEKVA"
     gene            19683..20516
                     /gene="mhpR"
                     /locus_tag="ACHKTW_002802"
     CDS             19683..20516
                     /gene="mhpR"
                     /locus_tag="ACHKTW_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HFO6463800.1"
                     /translation="MQNNEQTEYKTVRGLTRGLMLLNMLNKLDGGASVGLLAELSGLH
                     RTTVRRLLETLQEEGYVRRSPSDDSFRLTIKVRQLSEGFRDEQWISALAAPLLGDLLR
                     EVVWPTDVSTLDVDAMVVRETTHRFSRLSFHRAMVGRRLPLLKTASGLTWLAFCPEQE
                     RKELIEMLAARPGDDYQLAREPLKLQAILARARKEGYGQNYRGWDQEEKIASIAVPLR
                     SEQRVIGCLNLVYMASAMTIEQAAEKHLPALQRVAKQIEEGVESQAILVAGRRSGVHL
                     H"
     gene            20593..21675
                     /gene="lacI"
                     /locus_tag="ACHKTW_002803"
     CDS             20593..21675
                     /gene="lacI"
                     /locus_tag="ACHKTW_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414879.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor LacI"
                     /protein_id="HFO6463801.1"
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAE
                     LNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERS
                     GVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSII
                     FSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREG
                     DWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDT
                     EDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQVQAVKGNQLLPVSLVKRKTTLAPNT
                     QTASPRALADSLMQLARQVSRLESGQ"
     gene            21798..24872
                     /gene="lacZ"
                     /locus_tag="ACHKTW_002804"
     CDS             21798..24872
                     /gene="lacZ"
                     /locus_tag="ACHKTW_002804"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HFO6463802.1"
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEAR
                     TDRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPVADTVVVPSNWQMHGYDAPIYT
                     NVTYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWV
                     GYGQDSRLPSEFDLSTFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHK
                     PSTQISDFHVATHFNDDFSRAVLEAEVQMYGELRDELRVTVSLWQGETQVASGTAPFG
                     GEIIDERGGYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEASDVGFR
                     EVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSH
                     YPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRN
                     HPSVIIWSLGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVD
                     EDQPFPAVPKWSIKKWLSLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGG
                     FVWDWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQ
                     QQFFQFRLSGQTIEVTSEYLFRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIE
                     LPELPQPESAGQLWLTVRVVQPNATAWSEAGHISAWQQWRLAENLSVTLPSASHIIPQ
                     LTTSETDFCIELGNKRWQFNRQSGLLSQMWIGDEKQLLTPLRDQFTRAPLDNDIGVSE
                     ATRIDPNAWVERWKAAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKT
                     YRIDGSGQMAITVDVEVASDTPHPARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAA
                     CFDRWDLPLSDMYTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETS
                     HRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQK"
BASE COUNT         5888 a         6880 c         6415 g         5715 t
ORIGIN      
        1 agatgtccta gtctctcttt cattctcttc ttgtcaacat cacgacaact ttcgtaaaac
       61 actcctgccc cgcttcaggt atactgtgca ccttaattcc acaaacatca ggcagaccat
      121 gacagactta atccaacgcc ctcgtcgcct gcgcaaatct cctgcgctgc gcgctatgtt
      181 tgaagagaca acacttagcc ttaacgacct agtgttgccg atctttgttg aagaagaaat
      241 tgacgactac aaagccgttg aagccatgcc aggcgtgatg cgcattccag agaaacatct
      301 ggcacgcgaa attgaacgca tcgccaacgc cggtatccgt tccgtaatga cttttggcat
      361 ctctcaccat accgatgaaa ccggcagcga cgcctggcgg gaagatggac tggtggcgcg
      421 tatgtcgcgc atctgcaagc agactgtgcc agaaatgatc gtcatgtcag acacttgttt
      481 ctgtgaatac acttctcacg gtcactgcgg tgtgctgtgc gagcatggcg tcgacaacga
      541 cgcgactctg gaaaatttag gcaagcaagc cgtggttgca gctgctgcag gcgcagactt
      601 catcgcccct tccgccgcga tggacggcca ggtacaggcg attcgtcagg cgctggacgc
      661 tgcaggcttt aaagatacgg cgattatgtc gtattcgacc aagttcgcct cttcctttta
      721 tggtccgttc cgtgaagctg ccggaagcgc attaaaaggc gaccgcaaaa gctatcagat
      781 gaacccaatg aaccgtcgcg aggcgattcg tgaatcactg ctggatgaag cccagggcgc
      841 agactgcctg atggttaaac ctgctggagc atacctcgac atcgtgcgtg agctgcgtga
      901 acgtactgaa ttgccgattg gcgcgtatca ggtgagcggt gagtacgcga tgattaagtt
      961 cgccgcgctg gcgggtgcta tagatgaaga gaaagtcgtg ctcgaaagct taggttcaat
     1021 taagcgtgcg ggtgcggatc tgattttcag ctactttgcg ctggatttgg ctgagaagaa
     1081 gattctgcgt taattttttt cctctcagaa agggggagtt aacctgttga aagtacggtg
     1141 aacgccccct caccctaacc ctctccccaa gggggcgagg gaaccgtccg cactcgtatc
     1201 accccgcccg ataaaacggt ttatccccaa gaatcgtcgc ccgatgcata atccgccgct
     1261 gtggcaggta atcggcattg gcatagtgct gagtcacgcg gttatcccaa atcgcaatat
     1321 cattcggttg ccagcgccaa cgcacctgaa actccggttt agtgatatgg gcgaataaaa
     1381 agcctaacaa ggcttcgctc tctttctcgc tgacatcgac aattcgcgta gtaaagcctt
     1441 cattcacaaa cagcgcctgt ttaccgctca ccggatgcgt tcgcaccacc ggatgtagca
     1501 acggaggatt tttcgcgacc gcctcgcgcc agcgttgatg ttcctcctcg gttttgcggt
     1561 atttgtattc cgggaacgat ttacggaaat catgctccgc acgcagcccg ctcagcagct
     1621 ggcggaaggg aacagagagc gcctcatagg ccgcaatacc gctggtccag agcgtatcac
     1681 cgccggtcga aggtaattct ttagctgcca gaatcgcccc tgcgggtggc gtttcaataa
     1741 atgtcacatc ggtatgccag ttgtcgttat ccggcggatt atcgttatgg gtatccagca
     1801 cgatgatctc gtcaacccct tcggcatgag ggtaaacagg gtgaatatgc agttcgccaa
     1861 aacgctgggc cagcgcgcgt tgctgctgcg gcgtaatagt ttgatcgcgc agaaacacca
     1921 cctgatggcg cagcaccgca tggtaaagct gttcaaactg attatcgctt aacgggcgcg
     1981 tcaggtcggc acccgaaatt tgtgcgccga tatacggccc cagcggggta atgctcagac
     2041 gttcactcat tgtacttctc catgccaggg cgtcagacgg cgctgtaacg cgcgcaaacc
     2101 cagttctaaa agaaaggcga taatcgcaat caccgcgatc cccgccagca ccacgtcagt
     2161 cgcgagaaat tcacccgctg actgaaccat aaatcctaaa ccgcgcgtcg cggcaatcag
     2221 ctccgccgct accagcgtag accagcccac acccagacca atgcgtaatc cggtgaggat
     2281 ttccggcagc gcgccgggca aaatgacaaa ccacagcacc tgcgcacggc tggcacccag
     2341 cgactgggcg gcacgaatgc gaacctgctg cgcgcttttc acccccgcca gcgccgacat
     2401 cgccaccggt gcaaaaatcg ctaaatagat cagtaagatc ttcgaggttt caccaatacc
     2461 aaaccagatc accatcagcg gcaaataagc cagcggcggc accggacgat aaagttcgat
     2521 taccggatcc agaatgccgc gcaccgtcgg gcttagcccc atggcgatcc cgaccggaat
     2581 accgatcacc accgccgcga gtagcgccag cacaatgcgc gtcaggctgg ctgccagatg
     2641 ctgccacagc gtggcgtcca taaatccctg cggtccggca atggtgagca gttttgccag
     2701 tacttgttgc agcggcggca aaaacagcgg gctaatcagt tgcagcgccg ccaccgtcca
     2761 ccatacagtg agtaaaaccg ctaacgtgcc aatgcttaag gtcacctgac gcgagagcgg
     2821 ccagcgccat ttcagccgcc gcgaatgcag tttttcatta atgagcacac tcatgagaac
     2881 gcctcccgtt gctcaaatac gcggcttaaa acatattcgc gcatggcgat gaattgtgga
     2941 tcggacttga tgctgcggct cgactctccc gcaacaaagc ggcgagcaaa gttgagcggc
     3001 agccgctcca gcacacggcc agggccggat gaaagcagaa ccagttcagt cgccataaac
     3061 accgcttctt ctatatcgtg ggtaatcaac agcacctgct tgcccgtctc ctgccagagt
     3121 ttcagcagca gagtttgcat ctggtcgcgg gtgaaggcgt ccagcgcacc aaacggttcg
     3181 tcgagtaata acagctgggg attcgccgcc agcgcgcggg caatccccac ccgctgacgt
     3241 tgaccaccgg aaagctgcca gatatagcgt ttttctgcgc cttccagccc cacttttttc
     3301 agcatctggt gcgcgatttc cagtcgctgc attttctcta tacctgccaa ttgcaggccg
     3361 aacgccacgt tgtcctgtac attgcgccac ggtagtagcc cttcattctg aaaaaccacc
     3421 ccgcgctctg ctcccggtcc ctcaatacgc ttacccgcca gttgaatgct gccatgctga
     3481 taaggcacaa aaccggcaat cagattcagc aacgtggttt taccgcagcc ggacggcccc
     3541 agcaccacca gtagctcgcc gctttccagc gtcaggttga tatcttccag tgccggtttg
     3601 ccgccataat cggcatagag atgagagatt tgcagcatct gcgcctcctt ttattgcacg
     3661 aagcgcgagg taacgtactg gctgtaatca ttcgctacag ccgggacctt gccctgctct
     3721 ttcaaaaact gcgcggtgtc gatgattgct ttgttcaccg gtccggtcag ttctgccgtt
     3781 tgttgctgcg gcgtcagata ggtatttccc ttcaccagcc ccggaacgtc accttcaggc
     3841 acgccgctta aacgcgccag tttgctgatg ttttccggct gtttcagcca cgcgtctggg
     3901 ttagcaatgt acggttgctg agcatcgatg gcgcttttag cgaacgcttt cacgacctca
     3961 ggatgtttct cggcaaaatc tttgcgcacc acccagacgt ccagcgttgg cgcaccccac
     4021 tgcccgacct gttcagaatc ggtcaacacc ttgccgtctt tttccagggc gttaaccgcc
     4081 ggtgcccaga cataagcacc atcaatatct ccccgctgcc aggcagcgat aatcgcgggc
     4141 ggctgcaggt tcacaatctc cacttgcccg ggtttaatgc cccagtgttt cagcgccgcc
     4201 agcaggctgt agtgggtggt agagataaac ggtacggcta tgcgtttgcc aatcagatct
     4261 tccggtttgc tgatagtttt ctttaccacc agcgcttcgg agttacccag ttttgacgcc
     4321 agcaagaaaa cttcaatcgg cacctgttgg ctggctgcaa ccgctaacgg gctggaaccg
     4381 aggttgccga tttgcacatc gccagaagcc agcgcccgca cgatgctggc tccgctgtca
     4441 aacttacgcc agtccacggt tgctccgctt tctttagcaa aggtgttgtc ggcctgagcc
     4501 actttcgccg gttcggctga ggtttgatac gccacggtga cgttcaccgc ctgtgcctga
     4561 aaagcgatga atgccagtgc ggcaagaagt gtgttacgcg atgaaattgc catgattgtc
     4621 tgctcccctg tcttgttatg ggagcagtat tcaggaataa aaacattcat taaaagaatt
     4681 agtcgttatc gcacagatga ttttattctt agcaaaaaaa cggctaagga aagattaaat
     4741 atgcaattac tccgttctaa caattgggaa aattactaca cttcatttta gtgatgcata
     4801 aacaaccaca ttacgagatt tattacttca ttatttctat caatatcaat acatataata
     4861 aaactattat attcgcattg tatatatcac tgtatcattc tcaatcactc gcatattaca
     4921 ttcacataat gtatataaac aatacttgtt cacagaacat attttttaaa tgcaattaat
     4981 tgacaaacta aatcacatct aaatacaatg tataaaacaa aataactata cataccaaac
     5041 atacaaacac ttaacatatg aattgcataa aaacattacc cagtcgaacc tccgggtctg
     5101 aaagcccttg cttttcagtt attagcaaca tttaacattt ataaattaac atctggctgc
     5161 gccagagatt acgtctctga gtgcatcgtt gtgcttgtag tataataaat aaaatggagt
     5221 gtcattaagt ggataaggtt ttagattcag ccctcctttc ttcggcaaat aaaagaaagg
     5281 gtatccttgc tattggcgca catcctgacg atatagaatt aggctgtggc gcatcgcttg
     5341 ctcgtcttgc gcaaaaagga atttatatcg cagcagtggt gatgactacc ggcaactctg
     5401 gtacagatgg aataatcgat cgccatgaag aatcgcgcaa cgccctaaag atattagggt
     5461 gccaccaaac tattcatctt aattttgctg acacccgcgc tcatttacag ctcaatgata
     5521 tgatttccgc cctggaagac atcattaaaa atcaaattcc ttctgatgtt gaaatcatgc
     5581 gggtatatac catgcatgat gccgaccgcc atcaggatca tctcgctgtt tatcaagctt
     5641 caatggttgc ctgccgcact attccacaaa ttctcggcta cgaaacccca agtacctggc
     5701 tttcatttat gcctcaggtt tttgaatccg ttaaagaaga atatttcaca gtcaaacttg
     5761 ctgcattaaa aaaacataaa agccaggaac gacgtgatta tatgcgtcat gatcgcctgc
     5821 gtgcagttgc acaatttcgc gggcaacagg tcaatagcga tctgggtgaa ggctttgtaa
     5881 ttcataaaat gattctttga gcaaattgca tgaaaacctg gatatttatc tgtatgtcca
     5941 tagcaatgtt gctatggttt ttaagtacgc taagacgtaa acccagtcaa aagaaaggct
     6001 gtattgacgc cattatacct gcgtataacg aaggcccgtg tctggcgcag tcactggata
     6061 atctactgcg taacccttat ttttgccggg taatttgcgt taacgacggc tccacggaca
     6121 ataccgaagc ggtcatggcg gaagtcaaac gcaaatgggg cgaccgcttt gttgccgtca
     6181 cgcaaaaaaa taccggtaaa ggtggtgcgc tgatgaatgg cctcaattat gccacctgcg
     6241 accaggtttt tttaagtgat gccgacacct atgttccgcc cgatcaagac ggaatgggct
     6301 atatgctggc agaaattgag cgcggtgctg atgccgtagg cggcattccc tctactgcgt
     6361 tgaaaggcgc ggggctgtta ccgcacatcc gcgcgaccgt aaagttgccg atgattgtta
     6421 tgaagcgcac gctacagcag ctcctgggcg gcgcaccgtt tattatcagc ggtgcctgcg
     6481 ggatgttccg tactgatgta ttgcgtaagt tcggtttctc ggatcgtact aaagtcgaag
     6541 accttgatct cacctggaca ttagtggcaa acggctaccg tattcggcag gcgaatcgct
     6601 gcatcgtata cccacaggaa tgcaacagtc cgcgtgagga gtggcgtcgc tggcggcgtt
     6661 ggattgtggg atacgcggtc tgtatgcgcc tgcataaaag acttttattt agccgcttcg
     6721 gtatcttcag tatatttcct atgctgttgg ttgtgattta tggcgttggg atttatctca
     6781 ctacctggtt taatgaattc atcaccaccg ggccgcatgg tgtggtgttg gcaatgtttc
     6841 cgcttatctg gatcggcgta gtttgtgtta ttggtgcttt tagcgcctgg tttcatcgtt
     6901 gctggttgtt ggtgccttta gcgccgcttt ccgttgtgta tgtattatta gcttatgcca
     6961 tctggattat ttatggactt attgcctttt ttactggacg cgaacctcag cgcgacaaac
     7021 ccacccgcta ttccgcactg gtggaagcgt caaccgctta ttcccaacct tctgtcacag
     7081 gaactgaaaa actatctgaa gcttaatgtt aaagagaaaa atattcagat tgcagaccag
     7141 gtaattattg atgaaactgc aggtgaagtt gttatcggcg cgaatacccg tatttgtcat
     7201 ggtgccgtta ttcagggtcc ggtagtgatt ggcgcaaact gcctgatagg taattatgcg
     7261 tttattcgtc ctggcacaat aatcagcaat ggtgtaaaaa ttggttttgc caccgaaatt
     7321 aaaaatgcgg ttattgaatc ggaagcaacg attggtccgc aatgttttat tgccgactcg
     7381 gtagttgcaa accaggcata tttgggcgca caagtacgta ccagtaatca tcgtctggat
     7441 gaacagcctg tgtctgttcg aactccagag ggaattatcg ctaccggatg cgataaatta
     7501 ggttgttata tcgggcagcg ttcacgcctt ggcgtacaag ttataatttt gcctgggcga
     7561 attatttccc cgaacacgca acttggcccg cgcgtgattg tagaacgtaa tttacctgcc
     7621 ggaacttatt cactccgaca agaacttatc cgtacaggag attaaaatga taaaacggac
     7681 gttattagcg gcggccattt ttagcgcatt gcccgcttat gccgggttaa cttccattac
     7741 cgcgggctac gattttaccg attattctgg cgatcatggc aaccgtaatt tagcgtatgc
     7801 tgaactggtg gcgaaagttg aaaacgcaac gctgcttttt aatctttccc aggggcgtcg
     7861 tgattatgaa actgaacatt tcaatgccac tcgcggtcag ggtgcggtct ggtataaatg
     7921 gaataactgg ctgacaaccc gaacgggtat tgcctttgcg gataatacgc cggtctttgc
     7981 ccgccaggat tttcgtcagg atattaacct ggccctgttg ccaaaaacgc ttttcacgac
     8041 cggttatcgc tacactaaat attacgatga tgtcgaagtc gatgcctggc aaggcggcgt
     8101 atcactctat actggcccgg tgatcaccag ctaccgctat acccattatg actccagcga
     8161 tgcaggtggt agttatagca atatgatttc cgtgcgtctg aatgacccgc gcggcactgg
     8221 ttatacgcaa ctatggctaa gccgcggaac aggtgcttac acctatgact ggacgccaga
     8281 aacacgctac ggcagcatga agagcgtcag tctgcaacgt attcaaccgc tgactgagca
     8341 acttaatctc gggctgacgg caggtaaagt gtggtacgac accccaaccg atgattataa
     8401 cggtctgcaa cttgcagccc atctgacctg gaaattctga ttccttctgc cgcccgctat
     8461 ccggggcggc cttccctgcc gattagcccc ccctttcctc tttgttttcc gaccacattc
     8521 accggataaa ttttattctc cagtgttata tactataggg gggtatgcat tgacatatag
     8581 catacccccc tatagtatat tgcgtgcaga taatgaggtg cgaaatgccc agtactccgg
     8641 aagagaagaa aaaggtcctt actcgagttc gtcgtattcg agggcagatt gatgctctgg
     8701 aacggtcgct ggagggtgat gccgaatgcc gtgccatact ccaacagatc gctgccgttc
     8761 ggggcgcggc taatgggctg atggcagaag tgcttgaaag ccatatccgg gaaacgtttg
     8821 accgaaatga ctgctacagc cgcgaagtca gccaatccgt tgacgacact attgaactgg
     8881 ttcgagctta tcttaaatag ctgaatctat taccatattg aggaagagcg agagatgaaa
     8941 tcacgtgctg ccgttgcatt tgctcccggt aaaccgctgg aaatcgttga aattgacgtt
     9001 gcaccaccga aaaaaggtga agtgctgatt aaagtcaccc ataccggcgt ttgccatacc
     9061 gacgcattta ccctctctgg ggatgacccg gaaggtgtat tcccggtggt tctcggtcac
     9121 gaaggggccg gcgttgtggt tgaagtcggt gaaggcgtaa ccagcgtcaa acctggcgac
     9181 catgtgatcc cgctttacac cgcggagtgc ggcgagtgtg agttctgtcg ttctggcaaa
     9241 actaacctct gtgttgcggt tcgcgaaacc cagggtaaag gcttgatgcc agacggcacc
     9301 acccgttttt cttacaacgg gcagccgctt tatcactaca tgggatgctc aacattcagt
     9361 gaatacaccg tggtcgcgga agtgtctctg gccaaaatta atccagaagc aaaccatgaa
     9421 cacgtctgcc tgctgggctg tggcgtgacc accggtattg gcgcggtgca caacacagct
     9481 aaagtccagc caggtgattc tgttgccgtg tttggtcttg gcgcgattgg tctggcagtg
     9541 gttcagggcg cgcgtcaggc gaaagcggga cggattatcg ctatcgatac caacccgaag
     9601 aaattcgatc tggcgcgtcg cttcggtgct accgactgca ttaacccgaa tgactacgac
     9661 aaaccgatta aagatgtcct gctggatatc aacaaatggg gtatcgacca tacctttgaa
     9721 tgcatcggta acgtcaacgt gatgcgtgcg gcgctggaaa gtgcgcaccg cggctggggt
     9781 cagtcggtga tcatcggggt ggcaggttct ggtcaggaaa tctccacccg tccattccag
     9841 ttggtcactg gtcgcgtatg gaaaggttcc gcgtttggcg gcgtgaaagg tcgttcccag
     9901 ttaccgggta tggttgaaga tgcgatgaaa ggtgatatcg atctggaacc gtttgtcacg
     9961 cataccatga gccttgatga aattaatgac gccttcgacc tgatgcatga aggcaaatcc
    10021 attcgaaccg taattcgtta ctgatttccc gcaggtatac cccgtccact tcagacgggg
    10081 ttcttaatac tctccctggg cagccgtccg ggggattaac cgtgagataa tgactgatgg
    10141 aactcattga aaaacatgcc agctttggcg gctggcaaaa tgtgtatcgg cattattccc
    10201 aatcactgaa atgtgaaatg aatgtcggcg tctatctccc accaaaagcc gcgaatgaaa
    10261 aattgccggt gctgtactgg ctttcgggcc tgacctgcaa cgagcagaat ttcattacta
    10321 aatcggggat gcagcgttac gcggctgaac acaacattat tgttgttgcg ccggacacca
    10381 gtccgcgagg cagtcatgtc gcagatgccg accgttacga tctcgggcaa ggtgccgggt
    10441 tttacctgaa cgcaacgcaa gcgccgtgga atgaacatta caaaatgtat gactatatcc
    10501 gcaacgagct gccggattta gtgatgcaac attttccggc aacgaccaga aagtctatct
    10561 ctggtcattc tatgggcggg ctgggcgcgc tggtgctggc gttacgtaac ccagatgaat
    10621 atgtcagcgt ctcggcgttt tcgcccattg tctccccatc gcaagtaccg tggggacagc
    10681 aagcctttgc cgcatatctt ggtgaaaata aagatgcctg gttgaattac gatccggtga
    10741 gtcttatttc acaaggtcaa cgcgttgcgg aaatcatggt tgatcagggg ttgagtgatg
    10801 atttttacgc ggaacagctg cggactccaa atcttgaaaa gatctgccag gagatgaata
    10861 tcaagacgtt aatccgttat cacgagggtt acgaccacag ctattatttt gtctccagtt
    10921 ttattggcga gcatattgcc taccacgcca ataaactgaa tatgcgttga taatagtgca
    10981 cgactgccgg atgcggcgtg aacgccttat ccgcctacat ttcgcccgca ggccgtaggc
    11041 atgataagac gcgcatagcg tcgcatcagg catctgcgca cgacttatct atgtttacca
    11101 catcaaatca tcaggcactt tgaagtcggc atatgggtcg tcttcatcct gctcttcggc
    11161 actgagcgcg ctgtgcaaca caatactgct ggcatcacgc tgagcaattt tatcggcaac
    11221 gctggcggga ataatggcgt attcaccttc gctattgtta tcgaccaaca agcgggcaat
    11281 cgccagacga ccattaatca actgcgcctg tgtgagctta tcgacaaaaa tcttcttaat
    11341 cagattgccg tcagtgaagt taaaaccaat atcaccattg gcaatggtga ttcggttcat
    11401 ttcaataagc tgcttcacct gggctttata ttctttcgcc aacgccgctt gtttttgctg
    11461 ttcgctaagc tgtttatcac gctcaagctg tgccttttta ttttcttcta ccgccgcccg
    11521 agcttcacgc gcctgaacgc gcgatttttt cgccgttctc tcgaccttcg ccgctttttt
    11581 gctggtgact aatcctgctt tgagcaactg ctcttgtaag gtaagttttg ccatctttgt
    11641 ttccaaaccc gctgaataat ttttagcgag tatacctgta aacgccgtgg gtgtattggg
    11701 ggcaagtccc tttcgcccct gctaaataag gcgcgtcagg catcggcaca cggctgcatt
    11761 cttgatctcc gactcatcaa aataaacacc gccaacccag caacaagaat acccggtgca
    11821 gaagcggcca ttacgccgac cgtgccagtg cctaatgcca gcattttccc ggccagtaac
    11881 ggaccgctca tagcccccag acgccctacc gccacggctg ttcccacacc tgttgcgcgg
    11941 atctgcgaac tgtaaaacaa cggtgccagg gcatacaaaa cgctttgccc acctgtcgca
    12001 aacaaccccg cgacaaatcc cgccagcaac ataccgttaa atgacgacac cgttccaagc
    12061 gccagcagcg aagctaacat gccgctataa atcagtagcg acatggttac tggacgcagc
    12121 ttatccatca atgcgcccaa cattaacgtc ccgcttgccg cccccatttg cagggcaaac
    12181 atcacccctg ccgcctgcga tggctggaat ccttgctcca ccaaaagtag cggtagccag
    12241 ttgatcaaca tataaaccac cagcagagtg aagaaataac acaaccacag cagcagcgtc
    12301 gcggttgccg tttctggcgc aaataaggca cgcagtggtg gcgcagcctg tttttcgcca
    12361 gcgaaaaccg ctgactccgg cagccagcgc attaatagcg gcaccagaat caacggcacc
    12421 acaccaccta cccaaaacac cgtttgccat gctaagtttg cccccgcgaa acccagtgtc
    12481 gccgccagcg ccgcgccaat gggaacaccg caatacatca ggctcactgc cgtcccacga
    12541 aaacgtggac ccgcggcttc agacgtcagg gcgataagat tcggcaacgc cgcccccagc
    12601 ccgacaccgg tcatcagccg cgcaaagacc agtgagggga aatcccaggc aatcgccgtt
    12661 gccagtgaga acaaaccaaa cagcgcaact gagccaatca aaatactctt acgaccataa
    12721 cggtctgcca gcatcccgcc aaccaacgcg ccgggtagca aaccgagtat tccggcgcta
    12781 aatatccagc ccatttgcat tttatcgagt gcgaaagcct gggcgatgcc acccgccgca
    12841 atgccagccg cctgaagatc cagcccttcc atcagagcga ccaaaaaaca aagcccgatg
    12901 gtcagcatca ggcgggatga agatgatgaa ggggtacgag tcgacataag atgaacctcg
    12961 ttgcgagtgc aggtgaatcg cgactgatac attgccagcc gcgtaatcag aactatttaa
    13021 tgactgccta cgggcgaaat gtaggccgga caagacgttt atcgcatccg gcagtcgtgt
    13081 gcagatgcct gatgcgacgc taacgcgtct tatcaggcct acaaattctc acgccacccg
    13141 taggccggat aaggtgttta cgccgcatcc ggcagtcgtg ctgggatggg catacaaaat
    13201 gcacgttctc ggcagtcatc attatttgtt gttgcgcaga tccagcgcca cgtcaacgat
    13261 catatcctcc tggccgccaa ccatccggcg tttgcccagc tcaacgagaa tatccaccgc
    13321 acttaagcca taacgcgccg ccgccgtttc acagtgacgc aggaagctcg agtaaacacc
    13381 agcgtatccc agcgccagcg tttcgcgatc gactcgtacc ggtcgatcct gcaacggacg
    13441 caccaggtcg tcagcggcat ccattaacgc atagagatcg gtcccatgct gccagcccag
    13501 tttatccgcg gcggcaataa acacttccag cggtgcgtta cctgcgcccg cgcccattcc
    13561 cgcgaggctg gcgtcgattc ggtcgcagcc ctcttccacc gccgcgatag agttcgccac
    13621 gccaagactc aggttatggt gagcgtgcat gccagtttgc gtttctggtt tcagctctgc
    13681 tttcagggcg cggaaacggt cacggatatc gctcatgttc atcgcaccgc cagaatccac
    13741 cacataaata caggtcgcac cgtagccttc catcagcttt gcctgctttg cgagattctc
    13801 cggcgtggtc atatggctca tcatcagaaa accaacggtg tccattccga gctcgcgggc
    13861 atactgaata tgctgggcgg aaacatcagc ttcggtacag tgcgttgcca cacgaaccac
    13921 ccgcgcgcca gcctgccagg catttttcag atcgtgaata gtgccgattc ctggcagcaa
    13981 caacgtcgcg attttggcgt gcttcaccac atccgccgct gcttcaatcc attcaaggtc
    14041 gctatgtgcg ccgaaaccat agttaaagct ggaaccctgc aaaccgtcgc catgggccac
    14101 ttcaatcgaa tccacgcggg catcgtccag tgctttggca atctggcgaa cgttttccag
    14161 cgaatactga tgacgaatgg cgtgcatacc gtcacgcaac gtgacgtccg agatataaag
    14221 ttttttaccg ttcatgccgc ttctcctgcc ttgcgcgcca gtgactgggc cattttttcc
    14281 gctgtcgcca gcgcactgga agtcataatg tcgaggttgc ccgcataggc aggcagataa
    14341 tgcgctgcgc cttcgacttc cagccagacc gctgttttca gtccggagaa ttgccccacg
    14401 cccggtaaat tgaccggttt atcctgcggg ataacttcaa actgcacgcg ctgtttcagg
    14461 cgataacccg gtacgtaagc ctgcaccgcc tcagccattt cattgattga ggcttcgata
    14521 tcatcttgtg aagcttcgtc gctcaataca tacaccgtgt cacgcatcat caacggtggc
    14581 tctgctgggt taagcacaat aatcgccttc ccttttgccg cgccgcccac cacttcaatg
    14641 gctcgggaag tggtttccgt aaattcatcg atattggcac gcgtgccagg tccggcagat
    14701 ttactggcga tagaagcgat aatttcggcg taatgaacac gcgccacgcg tgaaactgcc
    14761 gccaccattg gaatggtggc ctggccgccg caggtgacca tgttgacgtt cagttgatcg
    14821 acgttcgcct cgaggttaac caccggcacg cagtaagggc cgatggcagc aggcgtcagg
    14881 tcaattaagc gaatatccgg tttcgcttcg cgtaaagcgg catcgttttt cacatgcgcg
    14941 cccgcgctgg tcgcatcaaa tacaatgtcg atatcagcaa attcaggcat gttcatcagc
    15001 ccgatcaccc cttcatgggt ggtggcgacg cccatacgtc tggcacgcgc cagaccgtcg
    15061 gactgaggat caatgccgac catcaccgcc atctccagat gctgaccgtg acgcaaaatt
    15121 ttaatcatca gatcggtgcc aatgttgcca gaaccgataa tggcgacttt acgcttactc
    15181 atgacagact tccttttggg gctgcgcttg aaaatgtcgc agcaactgaa cctatgcctt
    15241 caatatgggc ttcaaaacga tcgcccgcat tcactgccac catcggacct aatgccccgg
    15301 taagaatgat atctccggcg cgcagcggtt cacccaggct ggccattttg cgtgccagcc
    15361 agacggcggc attaagcgga tgtcccaggc attcgctgcc gcgcccgctg gaaacctctt
    15421 cgttattacg cgtcatcttc atggcgcagt ttttcaggtc taaccccgcc ggacgttgcg
    15481 ccggaccgcc gatgacatac accccgcagg aagcgttatc tgccacggta tcgacaaact
    15541 gaatcgacca gtcgcgaatg cggctaccca ccacctccag cgccggaagt acccattcaa
    15601 tggcgttata cagttcgtcg aaggtgatat cggttgcggg caaatcgcgg ttcaacacca
    15661 gtgcgatctc cgcttcaatg cgggcttgca gaacacggga aaaaggaatg atttcgttat
    15721 cgccataaca catgtcggca aataatgtcc caaaatccgg ttgatcaacg cccagttgtt
    15781 gctgcacttt cggatgcgtc aggcccactt tacgccctac cacgcgacgc ccctgcgcaa
    15841 cgtcatattg cacatttatg tgctgaatgg cgtaggccgc ttcagcgtta tcgataccaa
    15901 tcagatcgcg cagcggcgca atcgcttcgc cctgctctgc ggcgcggcgt aaatccgccg
    15961 ccagttgctc aagagtatgc ttcgtcatga ccattcctta agggcgtgcg aggaaattca
    16021 gcaccaattg attgaaagcg tcggcatgtt cccactgcgc ccagtgacca cagtcgcgga
    16081 agatatgcag ttcagaaccg gcaatgccgg acagcagacg cagacccgca tccatcggca
    16141 caaagcggtc gttgcgcccc cagacaatca gggtttgcgc tttgatttcc gccagacgtg
    16201 ggccaaaatc cgggaactgt ttcggattag cttccaggct cttaacgaag ttttccaggt
    16261 gatcgcggcg cgacagcata ttattcaggc gcgcttcaaa cagggcgtcg gtcaaatcgc
    16321 tggtatcgaa aacgaagata tccatcatca gcttcaggtt ttcgatagtc ggctgacgat
    16381 aaagctgatt cagtcgctta ataccttcgg ttggcatcgg cgtaaacaaa ctcatgccgc
    16441 ccgtaccgcc gcccatcagc accagtttgc cgacgcgctc cggccagtta agggtgaacg
    16501 ccatagaact atggccgccc atcgagttgc ccagcaggtg gattttggcg atatccagtt
    16561 gatccaccac gcttttcagg attcgtgcat taagatccga tcgtgaacca ctattaacga
    16621 tcgaatcgct tttgccccaa cccggacaat ccagcaggat tacccgatag cccgcctcta
    16681 ccagcggatc gatattgcgg ctgaagttcg cccagccagt agcacccggg ccggaaccgt
    16741 gcagcaggac aacggtttcg tcgccttgtc cgcagtcatt aaaatggatg cgcagcgttt
    16801 tacccgcttc ttctacattc agaaaacggc tggtggcggc ttcggtttgt ggctgataac
    16861 tcatcatctt ctcctgcata ttcagttctc tgttctggcg cttaacgagc caaatccggc
    16921 aatccactcc gggattgggc ggtaataacg cccttcgcta cgccagttgc caaatgcaga
    16981 aatagcggca aaagcggcga cccaggtttt gatttcatgt gtcgactttc cggcaatggc
    17041 ggaaagctct tcgttactga cggcatccag ttcctgtatg cgtccctgct ccagcaaagt
    17101 catgaactgg ttatcccaaa tcgggttgag cggatgcaga gttctctgat cctcaacaaa
    17161 cttcacagcg gcgctaatca cccgttgctg acgcaattcg cgctcactgg cgggtaaatc
    17221 tttcccgctc cccaacagac ggtcgcgcat atgggcatcg gctttcgcca gttcgggcac
    17281 cggcggctga tgggaaagcc caccggaacc caggaacagc acgcgtttat tgagagtgct
    17341 ggtgaaacgt ccaatggctt cacccaacat gcgggtacgc tggaaaccgg gcagcggcgt
    17401 ggcgacaccg ttgatgaaca caggcagaac tggcacctta tccagcccac cgagcaggaa
    17461 ctccagcggc tgggagaacc cgtggtccac ctgcatacag taagaaacgg caagatcgat
    17521 cccgctcttc atgacggcat gcgcacaggc ctccgccagc tccacaggca cgggcagctc
    17581 tcctgccgca ctgccgaaat caccaattgc cgtcgctcca acgcctaaac agaacggtgg
    17641 catcacgtca tagaaaaagc cgttgtagtg atctggcgca aacagcacca ccagttcagg
    17701 ggagaaagcc gcaatacgct cgcgggcgct ggcaatcacg ccattgacct catcgagtac
    17761 ctcttgtgcc gggtcgacat accccaccag cggcgagtgg gaaagacaat gaagataagc
    17821 gtgcatatca ggctaccttt tcgacagaaa cgtcggcatc agggcgggtc agcgtcatca
    17881 ccgacgccag tttattcagg gtcttgccca gggtttgcgg aatggcggtg gcggcaacaa
    17941 agcgatccgg gcgcatcacc accagcgaag cattgtgttg tgcgaaccag ctacgcagac
    18001 gaccttgcgt atcgcccacg cgtagcacgc cgtcgtggtt atcctgtgcc gtatgaattt
    18061 gcacttccgg caccacctga ataaagcgtg tgcccaacgc gcgccactgc tggatttgct
    18121 cgtcgctcat cccccacagt ggattgcatc cccagccaat taccgcgaag ttcgcgccga
    18181 tcgcgttatc gagcagcgtc acgtcgccgt tttccagcgt gactttcggc tgaataaaca
    18241 tcttgccgac cggagagtgc ttcgcttcgc cctcacgtac cagcgcaccg ccgtaatatt
    18301 gcggcatcgg cttgaagcgc atttcgagga agtagcgttt tactggcggc agataattca
    18361 gcagccagga aacgccgtca cgtaacgtac cctgccagcg tttcggcgga gccagcacgt
    18421 tgcccgccgt cacggacagg tcaatcatcg ctttggcgtg atcgcgacgt tcttgttgat
    18481 aggtattgag cagcgcatcg cgggctttcc cctggataac caacgccagt ttccaggcga
    18541 ggttaaaggc gtcgcgcata ccactgttat agccctgccc ctgccacacc ggcatgatgt
    18601 gcgcggcatc gcccgccagc agtacgcgat caatacggaa acgttgcgcc agtcgcgcgt
    18661 tatgggtgta gacacgctgg cgaatcaatt caacattgtc cggattaggc agcactttgc
    18721 ttaacagctt gcgcatattt tgcggctcac gcagctgctc ttcggtttct cccggcatca
    18781 ccataaattc aaagcgacgt accgcatgag gcagcgcggc agaaacatac gggcgcaccg
    18841 gatcgcaaca caaatagata tgcggcgtac ttaacggatc gttggcgata tctaccacaa
    18901 tccactgatt tggcgcagtt ttaccttcaa acggcacatt cagggtgcga cgaacaaaac
    18961 ttgccccacc atcacaggcc accagccact gggctttgat cgtttcccgc tgcccttctg
    19021 ccgttttcag gtgcaaggtc acttcgtcat cttgctggct gaaggcctcc agctcgcggg
    19081 aaaacaagca gcgcacattc ggaaaacgcg acaacccttc cagcatcacc gcatcgacct
    19141 gtggctgaat aaaggcgtta cggcgcggcc agccaaattc atcggtcatt ggctgaatat
    19201 cagcaaaaca gcggcctttt ggggtgagaa aacgcatcgc gtgccacggt gtagtgtgcg
    19261 gcagaacatt ctcgaccagg ccgaccgact gcatggtgcg cagcgcctcg tcatcaatac
    19321 caatcgcacg cgggtagtcg atcaacttat cgagtttctc caccaccagc acgtcaatgc
    19381 ccatctgacc gagatagttc gccatcatca gcccgaccgg accggcacca gagatcgcca
    19441 cctgaacgct atggttaaca gcaggctgga tgtcagggtg ttgtattgcc atttcagtac
    19501 ctcacgactc ggacaaaatg ccgttgcgcg cacagtacgg cgcaacttat tttgttaaaa
    19561 acatgtaaat gattttttat tgtgcgctca gtataggaag ggtgttttcg gctacaatca
    19621 aaacatgccc gaatgtgcac caggtgcaca acgttgtttt aactatagaa atgtcaatta
    19681 atatgcagaa caatgagcag acggaataca aaaccgtgcg cggcttaacc cgcggtctaa
    19741 tgttattaaa tatgttaaat aaacttgatg gcggtgccag cgtcgggctg ctggcggaac
    19801 tcagcggcct gcatcgcacc actgtgcggc gactgctgga gacgctgcag gaagagggat
    19861 atgtccgccg tagcccctcc gatgacagtt ttcgtctgac catcaaagtg cggcaattaa
    19921 gcgaaggatt tcgtgacgaa cagtggattt ctgcactggc ggccccgctg ctgggcgatc
    19981 tgttgcgcga agtggtatgg ccaacagatg tgtccaccct ggatgttgat gcaatggtgg
    20041 tacgcgaaac cacccaccgt ttcagccgct tatcctttca ccgggcaatg gtcgggcgac
    20101 gtttaccgct cctgaaaacc gcctcgggcc tgacctggct ggccttttgc ccggaacaag
    20161 aacgcaagga attaatcgaa atgttagccg cccgccccgg tgatgactat caactggcac
    20221 gagaaccgtt aaagctgcaa gccattctgg cgcgcgcgcg caaagagggt tacggacaga
    20281 actaccgcgg ctgggatcag gaggagaaga tcgcctctat cgccgtaccg ctgcgcagtg
    20341 aacaacgggt gattggctgt ctgaatctgg tgtatatggc gagcgcaatg accattgaac
    20401 aggccgcgga aaagcatctt ccggcgctac aacgggtagc aaaacagatc gaagaagggg
    20461 ttgaatcgca ggctattctg gtggccggac ggcgaagcgg cgtgcattta cattgacacc
    20521 atcgaatggc gcaaaacctt tcgcggtatg gcatgatagc gcccggaaga gagtcaattc
    20581 agggtggtga atgtgaaacc agtaacgcta tacgatgtcg cagagtatgc cggagtctct
    20641 tatcagaccg tttcccgcgt ggtgaaccag gccagccacg tttctgcgaa aacgcgggaa
    20701 aaagtggaag cggcgatggc ggagctgaat tacattccca accgcgtggc acaacaactg
    20761 gcgggcaaac agtcgttgct gattggcgtt gccacctcca gtctggccct gcacgcgccg
    20821 tcgcaaattg tcgcggcgat taaatctcgc gccgatcaac tgggtgccag cgtggtggtg
    20881 tcgatggtag aacgaagcgg cgtcgaagcc tgtaaagcgg cggtgcacaa tcttctcgcg
    20941 caacgcgtca gtgggctgat cattaactat ccgctggatg accaggatgc cattgctgtg
    21001 gaagctgcct gcactaatgt tccggcgtta tttcttgatg tctctgacca gacacccatc
    21061 aacagtatta ttttctccca tgaagacggt acgcgactgg gcgtggagca tctggtcgca
    21121 ttgggtcacc agcaaatcgc gctgttagcg ggcccattaa gttctgtctc ggcgcgtctg
    21181 cgtctggctg gctggcataa atatctcact cgcaatcaaa ttcagccgat agcggaacgg
    21241 gaaggcgact ggagtgccat gtccggtttt caacaaacca tgcaaatgct gaatgagggc
    21301 atcgttccca ctgcgatgct ggttgccaac gatcagatgg cgctgggcgc aatgcgcgcc
    21361 attaccgagt ccgggctgcg cgttggtgcg gatatctcgg tagtgggata cgacgatacc
    21421 gaagacagct catgttatat cccgccgtta accaccatca aacaggattt tcgcctgctg
    21481 gggcaaacca gcgtggaccg cttgctgcaa ctctctcagg tccaggcggt gaagggcaat
    21541 cagctgttgc ccgtctcact ggtgaaaaga aaaaccaccc tggcgcccaa tacgcaaacc
    21601 gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaagt ttcccgactg
    21661 gaaagcgggc agtgagcgca acgcaattaa tgtgagtcag ctcactcatt aggcacccca
    21721 ggctttacac tttatgcgtc cggctcgtat gttgtgtgaa attgtgagcg aataacaatt
    21781 tcacacagga aacagctatg accatgatta cggattcact ggccgtcgta ttacaacgtc
    21841 gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg
    21901 ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc
    21961 tgaatggcga atggcgcttt gcctggtttc cggcaccaga agcggttccg gaaagctggc
    22021 tggagtgcga tcttcctgtc gccgatactg tcgtcgtccc ctcaaactgg cagatgcacg
    22081 gttacgacgc gcccatctac accaacgtga cctatcccat tacggtcaat ccgccatttg
    22141 ttcccacgga gaatccgact ggttgttact cgctcacatt taatgttgat gaaagctggc
    22201 tacaggaagg ccagacgcga attatttttg atggcgttaa ctcggcgttt catctgtggt
    22261 gcaacgggcg ctgggtcggt tacggccagg acagccgttt gccgtctgaa tttgacctga
    22321 gcacattttt acgcgccgga gaaaaccgcc tcgcggtgat ggtgctgcgc tggagtgacg
    22381 gcagttatct ggaagatcag gatatgtggc ggatgagcgg cattttccgt gacgtctcgt
    22441 tgctgcacaa accgagcaca caaatcagcg atttccatgt tgccactcac tttaatgatg
    22501 atttcagccg cgcggtactg gaagcagaag ttcagatgta tggcgagctg cgcgatgagc
    22561 tgcgggtgac ggtttctttg tggcagggtg aaacgcaggt cgccagcggc accgcgcctt
    22621 tcggcggtga aattatcgat gagcgtggtg gttatgccga tcgcgtcaca ctacgtctga
    22681 acgtcgaaaa cccgaaactg tggagcgccg aaatcccgaa tctctatcgt gcggtggttg
    22741 aactgcacac cgccgacggc acgctgattg aagcagaagc cagcgatgtc ggtttccgcg
    22801 aggtgcggat tgaaaatggt ctgctgctgc tgaacggcaa gccgttgctg attcgcggcg
    22861 ttaaccgtca cgagcatcat cctctgcatg gtcaggtcat ggatgagcag acgatggtgc
    22921 aggatatcct gctgatgaag cagaacaact ttaacgccgt gcgctgttcg cattatccga
    22981 accatccgct gtggtacacg ctgtgcgacc gctacggcct gtatgtggta gatgaagcca
    23041 acattgaaac ccacggcatg gtgccaatga atcgtctgac cgatgatccg cgctggctac
    23101 cggcgatgag cgaacgcgta acgcgaatgg tgcagcgcga tcgtaatcac ccgagtgtga
    23161 tcatctggtc gctgggaaat gagtcaggcc acggcgctaa tcacgacgcg ctgtatcgct
    23221 ggatcaaatc tgtcgatcca tcccgcccgg tgcagtatga aggcggcgga gccgacacca
    23281 cggccaccga tattatttgc ccgatgtacg cgcgcgtgga tgaagaccag cccttcccgg
    23341 ctgtgccgaa atggtccatc aaaaaatggc tttcgctacc tggagagacg cgcccgctga
    23401 tcctttgcga atacgcccac gcgatgggta acagtcttgg cggcttcgct aaatactggc
    23461 aggcgtttcg tcagtacccc cgtttacagg gcggcttcgt ctgggactgg gtggatcagt
    23521 cgctgattaa atatgatgaa aatggcaatc cgtggtcggc ttacggcggt gattttggcg
    23581 atacgccgaa cgatcgccag ttctgcatga acggtctggt ctttgccgac cgcacgccgc
    23641 atccagcgct gacggaagca aaacaccagc aacagttttt ccagttccgt ttatccgggc
    23701 aaaccatcga agtgaccagc gaatacctgt tccgtcatag cgataacgag ctcctgcact
    23761 ggatggtggc gctggatggc aagccgctgg caagcggtga agtgcctctg gatgtcgctc
    23821 cacaaggtaa acagttgatt gaactgcctg aactaccgca gccggagagc gccgggcaac
    23881 tctggctcac agtacgcgta gtgcaaccga acgcgaccgc atggtcagaa gccgggcaca
    23941 tcagcgcctg gcagcagtgg cgtctggcgg aaaacctcag tgtgacgctc ccctccgcgt
    24001 cccatatcat cccgcaacta accaccagcg aaacggattt ttgcatcgag ctgggtaata
    24061 agcgttggca atttaaccgt cagtcaggcc ttctttcaca gatgtggatt ggcgatgaaa
    24121 aacaactgct gacgccgctg cgcgatcagt tcacccgcgc gccgctggat aacgacattg
    24181 gcgtaagtga agcgacccgc attgacccta acgcctgggt cgaacgctgg aaggcggcgg
    24241 gccattacca ggccgaagca gcgttgttgc agtgcacggc agatacactt gctgatgcgg
    24301 tgctgattac gaccgctcac gcgtggcagc atcaggggaa aaccttattt atcagccgga
    24361 aaacctaccg gattgatggt agtggtcaaa tggcgattac cgttgatgtt gaagtggcga
    24421 gcgatacacc gcatccggcg cggattggcc tgaactgcca gctggcgcag gtagcagagc
    24481 gggtaaactg gctcggatta gggccgcaag aaaactatcc cgaccgcctt actgccgcct
    24541 gttttgaccg ctgggatctg ccattgtcag acatgtatac cccgtacgtc ttcccgagcg
    24601 aaaacggtct gcgctgcggg acgcgcgaat tgaattatgg cccacaccag tggcgcggcg
    24661 acttccagtt caacatcagc cgctacagtc aacagcaact gatggaaacc agccatcgcc
    24721 atctgctgca cgcagaagaa ggcacatggc tgaatatcga cggtttccat atggggattg
    24781 gtggcgacga ctcctggagc ccgtcagtgt cggcggaatt ccagctgagc gccggtcgct
    24841 accattacca gttggtctgg tgtcaaaaat aataataacc gggcaggcca tgtctgcc
//
LOCUS       DAZFQD010000068        24848 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.068, whole genome shotgun sequence.
ACCESSION   DAZFQD010000068 DAZFQD010000000
VERSION     DAZFQD010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24848
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.068"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            15..863
                     /locus_tag="ACHKTW_002805"
     CDS             15..863
                     /locus_tag="ACHKTW_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000037410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA C-terminal domain-containing protein"
                     /protein_id="HFO6463803.1"
                     /translation="MLEAFIKEHPEEKNAAQIIGERLRYTIDAPSLKKENINKLTSAD
                     LDKIKANVDAVAKTITSETAPVYREAIKNGMTSEDALAVVNQDKTGGLLKQFNGLNTN
                     VNALKDTTTFALDENGEITDGQGVETVSVKSVVADVKADTTIYQNKDGSYTLDQSAPG
                     NVRVNDAVVSLDNRTRSNTQAIQNHSRQLQEHNARLNSQQRQIRENHEEMKRAAAQSA
                     ALSGLFQPYSVGKFNATAALGGYSDKQAVAVGVGYRFNEQTAAKAGIAASDGDVSYNV
                     GVNFEF"
     gene            954..1427
                     /locus_tag="ACHKTW_002806"
     CDS             954..1427
                     /locus_tag="ACHKTW_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6463804.1"
                     /translation="MKVILATRNRYLEYGLQALLKEHSVILAREFFLPENRRYIPDFD
                     ESWLIICDTLLGRLMRCMFQGRHFLQLGAESLRDGEQISDAIRNGVWTYNSVARPLTM
                     SEMVVMFGYVYRQSRPCRLASEMGIHTKTVNTFLYTGMAKNGLYGVSVKQIACAE"
     gene            1432..1605
                     /locus_tag="ACHKTW_002807"
     CDS             1432..1605
                     /locus_tag="ACHKTW_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6463805.1"
                     /translation="MLPDLQRLQVIQGQMMNKDKCRMYGRKRYTAGAMCFMLFVWLGG
                     VVTLCAVAAIIMQ"
     gene            complement(1642..1890)
                     /locus_tag="ACHKTW_002808"
     CDS             complement(1642..1890)
                     /locus_tag="ACHKTW_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFO6463806.1"
                     /translation="MRVEICIAKEKISKMPKGSVSALQQEMLRRVSKRYDDVDVIVRA
                     ANNDSLTILRAVDKESAHEFVQETLQEAWETADDWFIR"
     gene            complement(2118..2183)
                     /locus_tag="ACHKTW_002809"
                     /pseudo
     CDS             complement(2118..2183)
                     /locus_tag="ACHKTW_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2405..4090
                     /gene="btsS"
                     /locus_tag="ACHKTW_002810"
     CDS             2405..4090
                     /gene="btsS"
                     /locus_tag="ACHKTW_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HFO6463807.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISST
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
     gene            4087..4806
                     /gene="btsR"
                     /locus_tag="ACHKTW_002811"
                     /gene_synonym="yehT"
     CDS             4087..4806
                     /gene="btsR"
                     /locus_tag="ACHKTW_002811"
                     /gene_synonym="yehT"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HFO6463808.1"
                     /translation="MIKVLIVDDEPLARENLRVFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLEPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            4853..5323
                     /gene="yehS"
                     /locus_tag="ACHKTW_002812"
     CDS             4853..5323
                     /gene="yehS"
                     /locus_tag="ACHKTW_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HFO6463809.1"
                     /translation="MLSNDILRSVRYILKANNNDLVRILALGNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHVKKG"
     gene            complement(5363..5824)
                     /gene="yehR"
                     /locus_tag="ACHKTW_002813"
     CDS             complement(5363..5824)
                     /gene="yehR"
                     /locus_tag="ACHKTW_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HFO6463810.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASIGATTKEDAAKTLEPLSAKYKNIAGVEEKLTYTDTYA
                     QENVTIDMEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGFKEVK"
     gene            complement(5949..7949)
                     /gene="yehQ"
                     /locus_tag="ACHKTW_002814"
     CDS             complement(5949..7949)
                     /gene="yehQ"
                     /locus_tag="ACHKTW_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HFO6463811.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     GALIATFSDGVRTQLANSQALKEAQCSCGASGMCRHRVMLVLSYQRLCATVQPTEKEE
                     EWDPAIWLEELATLPDATRKRAQALVAKGITIELFCAPGEIPSARLPMSDVRFYSRSS
                     IRFARCDCIEGTLCEHVVLAVQAFVEAKAQQAEFNHLIWQMRSEHVTSSDDPFASEEG
                     QTCRQYVQQLSQALWLGGISQPLIHYEAAFNRALQAAEACNWRWVSESLRQLRASVDA
                     FHTRASHYHAGECLRQLAALNSRLNCAQEMARRDSVGEVPPVPWRTVVGSGIAGEAKL
                     DHLRLVSLGMRCWQDIEHYGLRIWFTDPDTGSILHLSRSWPRSEQENSPAATRRLFSF
                     QAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWQMLSAPLRQPGIVALR
                     EYLRQRPPACIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEGEDNLLTLSL
                     PASACSPFAVERMAALLQQTDDPVSLVSGFVSFVDGQLTLEPRVMMTKTRAWALDAET
                     APVAPLPSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAISQAELLANDLSAV
                     GFYRLAHVLGQFRNTESEARVEAMNNGVLLCEQLFPMLQQQG"
     gene            complement(7946..9082)
                     /gene="yehP"
                     /locus_tag="ACHKTW_002815"
     CDS             complement(7946..9082)
                     /gene="yehP"
                     /locus_tag="ACHKTW_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HFO6463812.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSGGLGGSNLTTPEWINSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDVLERMQPSESLLRAVLHTKHLMNPEVLAAARQIVRQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSLIPLARNFDFKSTLRANLQHWHPQHGKLYIESPRFNSRIKRQSEQWQLV
                     LLVDQSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAVEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDRDTAQALVNVGAQIAAMTPGELASWLAENLQS"
     gene            complement(9075..11354)
                     /gene="yehM"
                     /locus_tag="ACHKTW_002816"
                     /pseudo
     CDS             complement(9075..11354)
                     /gene="yehM"
                     /locus_tag="ACHKTW_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416624.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5682 family protein"
     gene            complement(11365..12453)
                     /gene="yehL"
                     /locus_tag="ACHKTW_002817"
     CDS             complement(11365..12453)
                     /gene="yehL"
                     /locus_tag="ACHKTW_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HFO6463813.1"
                     /translation="MSPQNNHLQRPPAAVLYADELAKLKQNDNAPCPPGWQLSLPAAR
                     AFILGDSAQNISRKVVISPSAVERMLVTLATGRGLMLVGEPGTAKSLLSELLATAISG
                     DAGLTIQGGASTTEDQIKYGWNYALLINHGPSTEALVPAPLYQGMRDGKIVRFEEITR
                     TPLEVQDCLLGMLSDRVMTVPELTGEASQLYAREGFNIIATANTRDRGVNEMSAALKR
                     RFDFETVFPIMDFAQELELVASASARLLAHSGIPHKVPDAVLELLVRTFRDLRANGEK
                     KTSMDTLTAIMSTAEAVNVAHAVGVRAWFLANRAGEPADLVDCIAGTIVKDNEEDRAR
                     LRRYFEQRVATHKEAHWQAYYQARHRLP"
     gene            complement(13026..13346)
                     /gene="yehK"
                     /locus_tag="ACHKTW_002818"
     CDS             complement(13026..13346)
                     /gene="yehK"
                     /locus_tag="ACHKTW_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000636926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YehK"
                     /protein_id="HFO6463814.1"
                     /translation="MIVQKELVAIYDYEVPVPEDPFSFRLEIHKCSELFTGSVYRLER
                     FRLRPTFHQRDREDADPLINDALIYIRDECIDERKLRGESPETVIAIFNRELQNIFNQ
                     LIEY"
     gene            complement(13407..17039)
                     /gene="yehI"
                     /locus_tag="ACHKTW_002819"
                     /pseudo
     CDS             complement(13407..17039)
                     /gene="yehI"
                     /locus_tag="ACHKTW_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416621.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein YehI"
     gene            complement(17049..20842)
                     /locus_tag="ACHKTW_002820"
                     /pseudo
     CDS             complement(17049..20842)
                     /locus_tag="ACHKTW_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
     gene            complement(20983..23016)
                     /gene="metG"
                     /locus_tag="ACHKTW_002821"
     CDS             complement(20983..23016)
                     /gene="metG"
                     /locus_tag="ACHKTW_002821"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFO6463815.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPQLYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKAAAAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            23148..24257
                     /gene="apbC"
                     /locus_tag="ACHKTW_002822"
     CDS             23148..24257
                     /gene="apbC"
                     /locus_tag="ACHKTW_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HFO6463816.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            24520..24801
                     /gene="yehE"
                     /locus_tag="ACHKTW_002823"
     CDS             24520..24801
                     /gene="yehE"
                     /locus_tag="ACHKTW_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HFO6463817.1"
                     /translation="MNKYWLSGIIFLAYGLASPAFSSETATLAINGRISPPTCSMAMV
                     NGQPQQHCGQLTYNVDTRHLFSSPVKGVTTEVVVAGSDSKRRIVLNRYD"
BASE COUNT         5988 a         6161 c         6446 g         6253 t
ORIGIN      
        1 atgaagttaa ttcattgctc gaggctttta ttaaggagca tccagaagaa aagaacgcag
       61 ctcagattat tggtgagcgc ttgcgttata caattgatgc gccatcttta aaaaaagaaa
      121 atattaacaa attaacatct gccgatcttg ataaaataaa ggctaatgta gacgctgttg
      181 caaaaactat cacatcagaa accgcacctg tttatcgcga agctattaaa aacggcatga
      241 catcagaaga tgcgttggca gtggtaaatc aggataagac ggggggattg ctgaagcaat
      301 tcaacggtct taatacaaat gtaaatgcac tgaaagacac cactacattt gctctcgatg
      361 aaaatggtga aattactgat ggacagggcg ttgaaactgt atcagtgaag agcgttgttg
      421 cagatgtgaa agccgataca acaatttatc agaataaaga tggcagctat acacttgacc
      481 agagcgcacc gggcaacgtt cgtgtgaatg atgctgttgt cagcctcgac aacagaaccc
      541 gcagtaacac ccaggctatc cagaatcact cccgccagct gcaggagcat aacgcacgcc
      601 tgaacagcca gcagcgccag attcgtgaaa accacgaaga aatgaaacgt gcggcagcac
      661 agagtgcggc gctgtccggt ctgttccagc cgtacagtgt gggtaaattc aacgccacgg
      721 cagccctggg tggttacagt gacaaacagg ctgtggctgt tggtgtgggt taccgcttca
      781 acgagcagac cgcagccaaa gcaggtattg cagccagcga cggtgatgtt tcctacaacg
      841 taggtgtgaa cttcgagttt taattgctga cagtgacatg aagcatgacg gcaggcacag
      901 gagttttgcc tgccgttttt tttcatgagg cataaaaaca ggaaggtgct gcgatgaagg
      961 tgattctggc taccaggaat cgctatctgg aatatggcct gcaggcgtta ctgaaggaac
     1021 acagtgtgat actggcgagg gagtttttcc tgccggagaa ccgccgttat atcccggact
     1081 ttgacgaatc ctggctgata atctgtgata cgttgctggg cagactgatg cgctgcatgt
     1141 tccagggacg gcattttctg caactgggtg cggaatcact ccgtgatggt gagcagataa
     1201 gtgatgccat acgcaatggt gtatggacgt ataacagcgt tgcccgtccg ctgacgatgt
     1261 cagagatggt ggtgatgttt ggctacgtct accgccagtc gagaccctgt cgccttgcca
     1321 gtgaaatggg cattcacacg aagacggtga atacctttct gtatacggga atggcgaaaa
     1381 acgggctgta tggtgtgagc gtaaagcaga ttgcttgtgc ggagtgattc agtgttaccg
     1441 gacctgcagc gtcttcaggt gattcagggg cagatgatga ataaagacaa atgccgtatg
     1501 tacgggagaa aacgttatac agcgggagca atgtgcttca tgctgtttgt atggctgggt
     1561 ggtgtggtga cactctgtgc cgttgctgca attatcatgc aataaaaaat ggggagtaag
     1621 ttaactcccc gtaaagaaga tttaccgaat aaaccagtcg tcagcggttt cccaggcttc
     1681 ctgcagtgtt tcctgaacaa attcgtgcgc ggattcttta tctacagctc ttaaaatggt
     1741 gagactgtca ttgttggcgg ctctgacaat cacgtccaca tcgtcataac gtttactgac
     1801 acgtcgaagc atttcttgct gcagggcaga aacagaacct tttggcattt tgctgatttt
     1861 ttctttagcg atacagatct caacacgcat aacaccctcc tgtaactgtg gttatgtaca
     1921 ggtgttattt ttgtctgtat ggataaccag tgtcaattcc tgatattgtt tatggggcat
     1981 caatggggca tgtatgggac actttttatc ggcgaaattc gtcgaagttc gtcgacatgg
     2041 taaacgaatc atctatccaa cccttgaaaa acggcgctcc tggacgatct tcgtcgattt
     2101 ttaaaaatgt tgcgtcacgc gcgtaacgtg acagggttaa tatcacaaag caacgccact
     2161 tcaccaattg tgtaaagcgc catcgtctca cccttgctcg cgaggtcccg gtttaacttt
     2221 agacgcagtt ttgcgaacca ggtagttttg cccgtttttt gtgcatctat agggtgattt
     2281 tatttttgcc aggcgatttt gagtgatcgt actcacgaat tctcattttt ctgcaagagt
     2341 tcaaagaaag ttaaacgcag gcaatgtatg ttacgcgttt taaagggaag tgtggtttgc
     2401 gggtatgtac gattttaatc tggtgttgct gctgcttcag cagatgtgcg tttttttagt
     2461 cattgcgtgg ttaatgagta aaacgccatt attcataccg ttaatgcagg tcacggttcg
     2521 tctgccgcat aaatttctct gctacatcgt cttttccatc ttctgcatca tgggcacctg
     2581 gtttgggttg cacattgacg attctattgc caatacccgt gcgataggcg cggtaatggg
     2641 cggcttactc ggcggtccgg tcgtcggtgg gctggttggt ctgaccggcg gcttacatcg
     2701 atattcgatg gggggcatga ccgcgctaag ttgcatgatc tcaaccatcg ttgaaggatt
     2761 gctcggcggc ctggtacaca gcatcctgat ccgtcgcggg cgcactgata aagtctttaa
     2821 ccccattacc gccggtgccg tcacgttcgt cgctgaaatg gtgcaaatgc tgatcatcct
     2881 tgcgatcgcc cgaccttatg aagatgcggt gcgtctggtg agtaatattg ctgcaccaat
     2941 gatggtcacc aataccgtcg gcgcggcgct gtttatgcgt atattgctcg ataaacgcgc
     3001 gatgtttgaa aaatacactt ctgctttttc tgccactgcg ctgaaagtgg cagcctcgac
     3061 ggaaggcatt ttgcgacagg ggtttaacga agtgaacagc atgaaagtgg ctcaggtgct
     3121 gtatcaggag ctggatattg gtgcagtcgc gattaccgat cgagagaaat tgctggcctt
     3181 taccggaatt ggtgacgacc accatttacc cggcaaaccg atttcttcga cttacacctt
     3241 aaaagcgatt gaaaccggtg aagtggtcta cgctgatggc aacgaagtac cttatcgttg
     3301 ctctttgcat ccgcaatgca aactggggtc gacgctggta attccgttgc gtggcgaaaa
     3361 tcagcgcgtg atgggcacca tcaaattgta tgaagccaaa aaccgtttat tcagttcaat
     3421 aaaccgcacg ctgggcgagg ggattgcgca actgctttcg gcgcagattc ttgccgggca
     3481 atatgagcgg caaaaagcga tgctcaccca gtcagaaatc aaactgcttc acgcccaggt
     3541 gaatcctcat tttttgttta atgcgcttaa caccattaaa gcggtgatcc gccgcgacag
     3601 cgaacaggcc agccagctgg tgcagtatct ttccactttt ttccgcaaaa acttaaagcg
     3661 gccttcggag tttgttactc tcgccgacga aattgaacat gtgaacgctt atctgcaaat
     3721 tgaaaaggcg cgcttccagt cgcggttgca ggtcaacatt gctattccgc aagaattatc
     3781 ccagcagcaa ttgcccgcgt ttaccctgca accgatagtg gaaaacgcca ttaaacatgg
     3841 gacatcacaa ctgctggata cagggcgagt ggcaatcagc gcccgacgtg aggggcaaca
     3901 tttgatgctg gagatcgaag acaatgccgg tttgtatcaa ccggtaacca atgccagtgg
     3961 gctggggatg aatctggtgg ataagcgttt acgtgaacgg tttggcgatg actatggaat
     4021 aagcgtcgcc tgtgagcctg atagttacac ccgaataacg ttacgactac catggaggga
     4081 cgaggcatga ttaaagtctt aattgtcgat gatgaaccgt tagcacggga gaacctgcgt
     4141 gtatttttgc aggagcagag cgatattgaa atcgttggag agtgttcaaa cgccgtggaa
     4201 gggatcggcg cggtgcataa actgcgcccg gatgtgctgt ttctcgatat ccagatgccg
     4261 cgcatcagtg gtctggaaat ggtggggatg ctcgagccgg aacatcgtcc gtatattgtt
     4321 tttctcaccg cgtttgacga atacgctatt aaagcctttg aagaacatgc ctttgattat
     4381 ctgctgaagc caattgatga agcgcgactg gagaaaacgc tggcgcgttt gcgtcaggaa
     4441 cgcagcaagc aggatgtttc gctgttaccg gaaaatcaac aggcgctgaa atttatccct
     4501 tgtacggggc atagtcggat ttatttgctg caaatgaaag atgtggcatt tgtcagcagt
     4561 cggatgagcg gtgtctacgt taccagccac gaagggaaag agggctttac cgaattgaca
     4621 ttacgtaccc tggaaagtcg tacaccacta ctgcgctgcc atcgtcagta tctggttaac
     4681 ctcgcgcatt tacaggagat tcgtctggaa gataacggcc aggccgagtt gattttgcgt
     4741 aatggcttaa ccgtgccggt cagccgccgt tatctgaaaa gcttaaaaga ggcgattggc
     4801 ctgtaaaaga ctgctaaaat ggctttttgc ctcatcaaca cctgaaggcc tcatgctaag
     4861 taacgatatt ctgcgcagcg tgcgctacat tttgaaagcc aataataatg acctggtgcg
     4921 tattctggcg ctgggtaatg tcgaagccac cgcggaacag atcgccgtct ggctacgtaa
     4981 agaagacgaa gagggttttc agcgttgtcc ggacattgtt ttgtcgtcat tcctcaatgg
     5041 cctgatttat gaaaaacgcg gcaaggatga gtctgctccg gcactggagc cggaacgtcg
     5101 cattaataac aacatcgtgc tgaaaaaatt acgcatcgcg ttttcgctga aaaccgatga
     5161 cattctggcg atcctcaccg aacagcagtt ccgcgtttcg atgccggaaa ttacagcgat
     5221 gatgcgtgca ccggatcata aaaacttccg cgaatgcggc gatcaatttt tacgttattt
     5281 tctgcgtgga ctggcagcgc gccagcatgt gaaaaaaggc taaaaaattg gcggtcatgt
     5341 ttagagcatg accgccaacc gattatttca cttctttaaa accagcggct ttcatcacca
     5401 gttccatttg cgccatagtg ataccttttt tggcatcttc agcagaaacg ttgattcctg
     5461 aaataccctg cagggcttta aaatccactt tttccatatc gatagtcacg ttttcctgcg
     5521 cgtaggtatc tgtataggtt aatttttctt caacacccgc gatgtttttg tatttggcgc
     5581 ttaacggctc aagtgtcttg gcagcgtctt ctttggtggt tgcaccaata gaagcaaatt
     5641 gaattttggt ttcagaagat tgcttaagca ccttgtcacc tttgtagaca taggtaatgg
     5701 caatttcagt gccgttcaga ttggcgctga atttcttcga ttcttctttg tcaccgcagc
     5761 cagcaagaga gaaaaccaga acagatgcaa caacgaggga aaacagctta ttgaaagcct
     5821 tcatgtaaaa ctccatttta tttaatcaag aaactggtga ctctcaccag gggctatata
     5881 gaatatgcct aataccgtga cgtgagcagt ccggaactgg agtagaactc ttagtaaaaa
     5941 gcactatttc atccttgttg ctgaagcatg gggaataatt gttcgcaaag taaaacaccg
     6001 ttattcattg cttctacccg tgcctcgctt tctgtattac gaaattgtcc caacacatgt
     6061 gccagccgat aaaaaccgac cgcggagagg tcattcgcca gcaactctgc ctgactaata
     6121 gcgctctgtt cctgatagcg ccagccgtta tggagcagtt gaataagtaa cgcctggcag
     6181 cgcatcagca actgatgagc ggtagacggc acaggcaaaa cgctggcaga aggtagcggt
     6241 gccacaggcg cagtttccgc gtccagcgcc caggcgcggg tttttgtcat catcacccgt
     6301 ggttccagtg tcaattgccc atcaacaaaa ctgacaaagc cagaaaccag actcacgggg
     6361 tcgtctgttt gttgcaaaag cgccgccatg cgttcaacgg caaaaggaga acaggcagat
     6421 gccggaaggg ataacgtcag gagattatct tcaccttcgc cgctgattac ctgcgcatcc
     6481 agcgtctggc ggctgctatc ccaaccgagc gaaatacact cagcgaccgg cagaataaat
     6541 aagttatcga cctgattaag aggccgtatg caggcggggg gacgctggcg taaatattcc
     6601 cgcaaagcca caatgcccgg ctggcgtaac ggcgcgctca acatttgcca ggcatcaggc
     6661 gacagcggca caacgctgct taagcggttg cgggtagcta acagcagctc gccatcggca
     6721 ctgcgttttg ctgcttgtga aacaatttgc ccacccgcca gtgcgccagc ctgaaaacta
     6781 aacagccgac gcgtagctgc cggtgagttt tcctgttcac ttcgcggcca actgcgcgaa
     6841 aggtgcaaaa tactgccggt gtcgggatcg gtaaaccaga tgcgtaaacc ataatgctca
     6901 atatcctgcc agcaacgcat acctaaagac accagccgca gatgatcaag ctttgcttct
     6961 ccggcaatgc cagagccaac gaccgtgcgc cacggcacag gaggaacttc accaacactg
     7021 tcgcgccggg ccatctcttg tgcgcaattt aatcgactgt ttaatgccgc aagctgacgt
     7081 aagcattctc cggcatgata gtggctggcg cgagtgtgga aggcatcaac gcttgcccgt
     7141 agctgtcgta gtgattcact cacccagcgc cagttgcagg cctctgccgc ctgcaatgcg
     7201 cggttgaatg ctgcctcgta gtgaataagc ggctggctga tgccgccaag ccataatgcc
     7261 tggcttaatt gctgaacata ttgacgacac gtttggcctt cttcgctggc aaacggatcg
     7321 tcagatgatg tgacgtgttc gctgcgcatc tgccagatta aatggttaaa ttctgcttgc
     7381 tgcgctttgg cctcgacgaa ggcctgcaca gccagtacga catgttcgca aagtgtgcct
     7441 tcaatacaat cacaacgggc gaaacgaata ctgctacggg aataaaaacg cacatcgctc
     7501 atcggtaagc gggcagaggg aatttcgccc ggcgcacaga acaactcaat ggtgatgcct
     7561 ttagcgacca gcgcctgcgc gcgtttgcgg gtggcatcgg gcagggtagc cagttcttcc
     7621 agccagattg ccggatccca ctcttcttct ttttccgtgg gctggacggt ggcacaaagt
     7681 cgttgataac ttaacaccag catcacgcga tgacggcaca tgccgctggc cccgcagctg
     7741 cactgagcct ctttcagtgc ctggctgttc gccagctggg tacggacacc gtcactgaag
     7801 gtggcgatta aagcgccgtt ctcatggctg atttccggga cgttgccatt ttccagttcc
     7861 ttaagactgc gcttaacaaa accggcattg cttaacgccg tcagtgcctg cggtgtcagt
     7921 tctaataatt ccggacgtag tgaattcatg actgaagatt ctccgcaagc catgatgcca
     7981 gctcgcccgg cgtcatggcg gctatttgtg cgccgacatt aaccagcgcc tgggccgtat
     8041 cgcggtcata gcaaggcgtt gcggtgctat cgagcgctgc cagtcccagc actttgatgc
     8101 cgctctggac acactttttc acctgatgcg tcagcaatga tgatgaaccc ccttcgtaaa
     8161 aatcgctcac gaggataatg acgctttttg ctggttgttc aataagttgc cgaccatact
     8221 ccacggcact ggcgatattg gtcccgccgc ccaactgtac tttcattaat aactctaccg
     8281 gatcggcaac gtctgccgtg agatcaacca cgcttgtgtc aaacgccacc agatgggtac
     8341 gaatgccggg taactgccac aaacaggccg ccatcaccgc agagtggatc accgagtcca
     8401 ccatcgatcc gctttgatca accagtaaga ccagttgcca ttgttcgctt tggcgtttaa
     8461 tgcggctgtt aaagcggggg gattcgatat acaacttgcc gtgttgcggg tgccagtgtt
     8521 gcaggttggc gcgcagagta cttttgaaat caaagtttcg cgccagtgga attaatgagc
     8581 ggcgacggcg atcgcggaca ccagaaaaag cctgacgaac ttcctttgcc agtcgcgcca
     8641 taatttcttc aacaacctgg cgcactatct ggcgggcggc agccagtact tcgggattca
     8701 tcagatgttt ggtgtgcaaa acggcgcgta gcaggctttc cgaaggctgc atacgttcca
     8761 gcacgtcgag atttgtcacc acatcttcaa tgccgtagcg cagtacggca tcgctttcca
     8821 gccgctcaat cacctgttgc ggaaacagcg tgtggatact gttgatccac tcaggagtgg
     8881 tgagatttga gccacctaat ccaccagagc gttcaccacg ctggagccgt tcaggatcgc
     8941 gcccatacag ccactccagc gcgtgatcta tctgccgggc gttgtcatcc agcccacaaa
     9001 gcgtcgtttc tgccgcttcg ccaagaatta atcgccagcg ttgtagctca cgggtggtca
     9061 gaagatcgtt cagttcagac atggaaaact ccccagtgtt gcagcgatgc cagtgcctgc
     9121 tgttcgagtt gttgatgatg tgcaatggct tgcggtggac aatgcaacgg catttgcagt
     9181 gccgaaacgg gaagttgcgc cagttgataa tgctcaagca cctgatgcgc cagcgttccg
     9241 cgttctcgtg gcggtagcca ggccatcgcc gcgcgtaaat cgggcagggc attgataaaa
     9301 ttggcatcac tcagttgatt caaatggctg ctgaaaccgg cgataaatgc aggttgacag
     9361 gccagttgat gacgggctaa tgccagcaaa ccgtgcagcg cctcgccgga ttgtgccggg
     9421 gataactgcg ccagcatcgt cagcgccgct tcggcactgg cgttgggatg ttccagacgc
     9481 attagtgcgc caagagccgc gccgcgatcc agcgccgggg catgaatcgc ctgactgcgt
     9541 cgctccagta aggctaccgc cgcagaaagc gaaacgccgg ataaattaac gccgctatgt
     9601 agatcgcgca gaatatggca aagcgcttgc cagctatgca ggtgagcgtg aaactccttt
     9661 tcatccggtc tgccgttaga ttcacacagc cacagcgtgc gatcgatagc cgcgcataac
     9721 gtcgtttgta atatctgcgc gccttgcata ccgctaattt catccagccg ccataaggca
     9781 tatagcactt ccagcgcctg gcccatttcg gcaaattgat tttcctgggc gattaataat
     9841 gtgagttgct ccagtaattg ttgactgaag gacgccagac ccgctaacgc cgcctggcta
     9901 agacatgtgg tgatactgcc gattccaccc gcgtccagca tatcggcttc taatttatgg
     9961 cgtgcggctt cctgaagtgt tgcaccaaag catgcggcct caatcaatgc cgcatgctgg
    10021 ctaagcgggc gggttaattt ccagtgttct tcaccgttgc ctgcaagtgt cagtgtactt
    10081 cactgctggc gtacaatccc agggatctcc agtattgcca gccgatgtaa cacctgactt
    10141 tgcgccagcc catcgggggt aaagcgattt aaggtaagct ccgccggaag agagattgtg
    10201 gtacgttcca gttcgcaggt gacatctttt ggcagaggcg gttgtggcgt ggaaggggca
    10261 agtttaccga atccgtcacc cgctaatgtg tctatcaacg ttagcagaat cggatcggta
    10321 tcgggatgaa taacgccgcg atagctccac ggcaacggtg cattcagggc ttctttaatc
    10381 aacgagcctg ctatggcatc cagccagtca gtgcgtaacg gtagtgtatg accgcgcaac
    10441 tgtgccagtg ccattgcatg cagatgtgcg gcagccatat ccgcggtcga agcaggcaag
    10501 ttgtgctggc gcaaacgggt gagaaccgtt tttagcagtt gttcaccggc ctgctgtaag
    10561 ccccactgcc agcaccagtt ttgccagacc ggggcaggca taccggaaag gtatcctgcc
    10621 agcacatcaa ggcgcttttc actgtagggc gtgagataac aacctgtaat ggcatctgcc
    10681 agcgagggca attccggcgt gttaatgtcc tgcgggcatt cgcgccacat ttttgccagt
    10741 gccggagcgt gccagccacc gcagacgact aacacgtcgc cattattttg ctgcaccgcc
    10801 catgcaatcc agcgagccat aaaggcttcg cgctgacgat tgagtgcatc gccggggaaa
    10861 tcgccccgca gttgggcaaa atagtgcgcc agcgcagagg gtaatgcagt ttgctggctt
    10921 tcatcttcga acaagtgatc ccacagggta tcgctgttat ccatgcgggt ggcacgcagc
    10981 agtaaggcct ggctttcatc ttgcgtatca ggcgaatcgt cctcttcttc actctgcgcc
    11041 cagcaaggca aatcgatgaa gtaagtttgt gcctgaatac gacgtgcggc ttgtagcgcc
    11101 tgccactccg gcgaaaattc agcaaatggc gtccaggcac cacgcccggg ggctgcaccg
    11161 tcctgatact ggcaataact gtaaatagct accggaagct ggtgggaaaa aaaaagttcg
    11221 tctacccggt cattaaaatc agccgggcct tcaatcaaca cgtatcgtgg ccgctggctt
    11281 tcgattaacg atttcaccag ccgggcgcag gccggactat gatgccggat gccgacaatt
    11341 aacggctcgc tcatgcctct ttcctcacgg caggcggtgg cgggcttgat aataagcctg
    11401 ccagtgagct tctttatgtg tggcgacacg ctgttcgaag taacggcgca gacgagcgcg
    11461 atcttcttcg ttatctttga cgatggttcc ggcaatacag tccaccaggt cggcaggctc
    11521 ccccgcgcga ttcgctaaaa accaggcgcg gacgcctacg gcgtgggcca cgttcacggc
    11581 ttcagcggtg gacataatcg ctgtcagtgt atccattgac gttttctttt cgccgttggc
    11641 acgcagatcg cggaaggtgc gtaccagtaa ctccagtact gcgtcaggca ctttatgcgg
    11701 aataccgcta tgggccagca aacgcgccga agcactggcg accagttcca gctcctgggc
    11761 gaaatccata atcggaaaga cggtttcaaa atcgaagcga cgctttagcg cggcactcat
    11821 ctcattgacg ccgcgatcgc gggtattggc agtggcaata atattaaagc cttcccgcgc
    11881 atagagctga ctggcttcac cggtgagttc cggcaccgtc atcaccctgt cagaaagcat
    11941 acctaacaga cagtcttgta cttccagcgg tgtacgcgtt atctcttcaa aacggacgat
    12001 tttgccgtcg cgcattccct ggtaaagcgg ggcagggacc agtgcttccg ttgacggacc
    12061 atggttgata agcagggcgt agttccagcc gtacttgatt tgatcttcgg tagtagatgc
    12121 gccgccctgg atggttaacc cggcatcgcc gctaatagcg gttgccagta attcagacag
    12181 gagagatttt gcggtgccag gttcccccac caacatcaaa ccgcgcccgg tggcaagagt
    12241 gactaacatg cgttcgacag cggagggact aatcaccact ttacggctaa tattttgcgc
    12301 gctatcgcca aggataaaag cacgggccgc aggcaaactt aactgccaac cgggcgggca
    12361 aggtgcgtta tcattttgtt ttaattttgc cagttcatcg gcgtataaca cagcagcagg
    12421 cggacgctgc agatgattat tctgcggtga catatattca tccctgaaat gtattaatga
    12481 aataaacgat attttacgcg caataaatgt ttggcgcatt agttgtttga gcgaaaaata
    12541 tctggaaagc agatcacagt aaataaaaaa acctgctggt gaggcaggtt cttttattta
    12601 ctactttgat gttaagttac tgatagtgca acatggcatc tcaacgaacg aattataacc
    12661 acctctggac agtgttgcaa ttaagataat gagatttata atcctgactg ttgggaagtt
    12721 gcgccggatc gaacccattt caagtcctgg ctaccggacg ggtggtgccg caggtagtgc
    12781 tctgatccgg agtgcaacat ggcgactcaa caaacgtaaa ccagtatcga caggcagtgt
    12841 aagggtaagg tgctggtgtt gcgctcctta ataaggagag cggatggaca cgctgacaca
    12901 aaagttaacc gtgctcattg ccgtactgga gctattagtg tctctgttac ggttgattga
    12961 tttgttgaag taatgggcaa attgaaacgc aactaagggg gaaaaccgct tcccccttta
    13021 ataaactaat attctattag ttggttgaat atattctgta gttcacgatt aaaaattgct
    13081 attacagttt caggtgattc acctcgtaat ttccgctcat caatacactc atctcttata
    13141 taaatcaacg catcatttat tagcgggtca gcatcttctc gatcacgttg atgaaatgtt
    13201 ggacgtagcc ggaatcgctc cagtcgatag acggaacctg taaataattc agagcattta
    13261 tggatctcaa gtctgaagga aaacggatct tcaggtacag ggacctcata atcgtaaata
    13321 gcaaccagct ctttttgcac gatcatcgct ttccccgctg gtaagtcgtg ttgataacgg
    13381 gcagttgcat cactgcccgc tgtgttttaa tggaaaatga cgtcaggtgc gcttaacgcc
    13441 tcactcaaat caaacacatc aagttcggca aaagtgacgg atttattgtc actgtacact
    13501 cgaagagaac ggaagctcat tagcgctttg ctatcaacat tgtagtggtt aaaaccgggt
    13561 gaaaacttaa ggaccagccg ctactcaccc aggtcataga caaatgcgtc ctcatagcct
    13621 gactcccagc ctttgtatcg cattcccata agttgcccaa cggtagcaga tttaccttcc
    13681 cagcgattca aactattact ggctgactcg tcaggcgtga gcagcaccgt gcgccgtgtc
    13741 aactggcgaa gtggcggtat aatttcgtaa tcggcaaaaa gctgggcaaa ttcactgcgc
    13801 atttctgctg tcatttctaa cgggtgggca atgccaatca gagcatccgc aggcaggtca
    13861 attggctcat cctgcgcatt gcagaactcc ccctctgcgg ccacacgaaa ggcgttgagt
    13921 aaacgacgcg gttcatttgc cggatacacg ccccatatta atcgctgggt aaccaggcgg
    13981 gtaaagggat gattaacaaa cagagtatga aaatctgcca gcgaccagcg tcgggtggta
    14041 cgtagggcag cttcaagacg cgggagcagg tttttgctca cctgagtagc atcttttttt
    14101 agccctttta gtcgggccag tgcctcgggc gctttcagtt gatcgtcatc ggcgcgcaac
    14161 cggggaacgc ttttctggcg aacgttttgc tgatcaaaga taaccgggtt gaggttttca
    14221 tcgaagcgaa cggtaaactg ccggggacca aaatcaaaac tcaacgcctg cggatcatca
    14281 aggcccaggg ttgggactaa tctgtccgct agctcttcct ggcttagccc gcgattttct
    14341 gcgaccacct gaagaaattc cgccgcgtta tcacgtaagg ggcgggattt agcccgttgc
    14401 gatatatgat gcagctgtat cagcgccata tcattattca tcagggtaag gatgttcagg
    14461 ccgctgacag cacgggcaga tttgccttct tgtggccagg cgaggatctg tgtcgtcaga
    14521 tcccgtgccg tgctttcgtc accaaagaga cttaacgcca gaaatgccca gttgtctttt
    14581 gcgggggcac cagcttgttg ccacgcagtg aagagatccc aggcaaaagc tgccagcgtt
    14641 tgtggctggc agaacgtttt cagttgttcc agcccgctat aaaaacgtcc cccctgggta
    14701 aagcgcagca tttcgccgat aatttccaga gcgtcaccgg taacaggctg attattagtg
    14761 ataagtcgag gcctggacca cattgccgga tgccagaaat caggggcttt tggaatgcgt
    14821 gccgggtaaa tgtccattgg accctgttta agcaactgct ccagggcaga ccatacatct
    14881 gtacgctgcc agcggtttgc cacggtttgt agcaattcgc catgtccctg ttcgtacagt
    14941 aaacgcaggg caagtaatgc ggcttcgcta ttatcgctgg gtttggtaaa gacaagaggg
    15001 ataagtgcac tagccgtatg ttccggccat tgtaaaatcc actggcgagc cagatcacgc
    15061 tgcgccgcga aacgtcgcca gacgcgggca acaggcagcg ccagttctgt tgcgccaaaa
    15121 tttaaaatta acggaaatgt ttcttttgga cgatgtgaaa aagccaacag cagacctggt
    15181 agcgcatcca atccgaggat actgagaaaa tagtcctcac ccgcgaacag atgctttttc
    15241 tcaatgatac gcagccagaa ggtcaaagcc atctcacgcg gcaactgtgc caataaatac
    15301 aggttccaga cactcttttc cggacgataa cgtgagaagt tgtcattttc ccatatgtta
    15361 attaacgctt ctatgtgacc gctgcggagc gcctgttctg ctaattgtgc ttgcggggag
    15421 ttccagccat tcaggatctc ttcaacccgc ccctggtagt aatgccactg aacaatgagt
    15481 gaattgcgtg cagcatccag ttttccgggg agtatataat tcagaaacag ccatacatca
    15541 tccccaaaac ccagttcctg taatacaact cgtccacttt tttgttccat gcgttccgca
    15601 agagattccg tgctgaaata acgggcagac tcagtggcgg caagcctctc ttgttcgcct
    15661 ggttgccagt aaatttgcgg agttaattcg agcggtgcca aatcaagacg tggagctgtc
    15721 atttttttct tactacgcca cggtggtgaa acgagcattt caggcagact gctgttatcg
    15781 gtgctatcaa taaccggttt tggcggtgcc gataaataac tattcagcac tgccctggct
    15841 tgagtactta accacggcat caccctttct gctagttgtg gctgcgatgc tatcagcgtt
    15901 tgtaacagcg tacgccagta ttcatcgcgt gctttttgct gtgcaggcag ctttttgtct
    15961 tcgatgattg gataacctgg gcgggctggt ggttctttta acgccagcag ttctgccagt
    16021 gcggcgagtg tcgcatgggg gaagttttta ctgtatttag ccacacgttg caggctgggt
    16081 ttgtttttat cagcaactaa cagcaaggtg cggatgactt gcggatggtt aatttgtacc
    16141 agcagactgc cgcaatcttc tttatgggca tatatcgcga ggcgcggaac ggctgccagt
    16201 ccttgttcgc gcaaaagggc ggcgcaggcc gcgtagccga agtggttttc atgcgacatg
    16261 tagctggctt ctcgatcgct aaatacgtcc aggccccagc atcgttcgag tttttttacc
    16321 gccgtattat cagtagcaat gacctttaac cactcctttg aatggagact tgaccgggag
    16381 ctttcaagac tggcgagttc attggttatt tcaggttttt ccgggaggag tagtgcaata
    16441 aaaggacggc gaattttggg tattcctggc agtgcggcaa tgagtttgtc ggcgcaacgc
    16501 tgccagctgc tttcctctgc cagagagaga tgcttacgca gtcggagatc gaattcgtta
    16561 taggtttctg agcgataacc atagccataa tcctcgtcct gatactgcaa tgtcactagc
    16621 agaggatcac tctcataaca tcgtgtaata aactggcgtg caatcaccac ctcagtcgca
    16681 tattccagtc cctcttgttg gacgatttcg tcgagcaact gttggctgtc attttgcgcg
    16741 atacagacta gcgccgccat tgtccgggcc gggattgagg gtttgtgttc gccgatcaag
    16801 ttgatggcct gcgtaatggc ggactgccac tccagtgaaa aattctcata acgccagaca
    16861 atgttttttt tcttctcagc tttttgtagc aattcatcac ccagtgtctg aatgatgctt
    16921 tcagtgataa caggcactgg ttcgcccggc caattgcgat ggctgagtgg tgttttgcct
    16981 tttttatatt tcagttccag ttgggcgtta tccgccagcc acggtaattc cttgtccatg
    17041 ctctttcctt attcaaacag ggcttcaata tcgttaatca gctcgctggc ggtgatggca
    17101 tcgagtacgg agaactgcgc ttcctgtgtt gaattactcc cccagccata gctttctgct
    17161 ttgccttccc atagccacag cttgcttaag agttgctcgg cgctaagttc ggctggcgac
    17221 ataccaactg caaagccttc atcgatttcc atgattaacg accagcggcc cagaggtttg
    17281 atcatccagc cgatccagcc accatcctgc ggttcaccac gcacccaacc tttattcgcc
    17341 agtcccatga cccgaccgct cgggcatttt ctgcctgccc agcgggtcag ttcactggca
    17401 ttgcgctcgg cttctgtcag ggcgtagctg ttacggtcga gctggcggaa cggtggtagc
    17461 agttcgtagt cggcaaaaag ctgaccaaag gcggcagcat ccgttggtga tatttccaga
    17521 acgtgaggag tgccgataga gatatcgcct tccggcaggg tgaaaagatc atcgtcagcg
    17581 gtgctgtagc tgttatcttc tgctacgcga aagcaagcca gtagctggtt ttcggcgcta
    17641 taaacgcccc aaatcagacg gcgggttaag tgacgaacca gcggatgctc aaccaggaag
    17701 agctggaagt tttccagcga ccagcggcgg cgcaggcaca tggcggattc cagccttgct
    17761 acctgctggg cggcgatggt acgcgcatct tttttcagca atttgtagcg gttaaccgca
    17821 tcgttcgccc gcgtttcatc atcgcttttg ttcggtttgg gcaggtcttt caggcggctg
    17881 ccggaaacat cacgcacaaa cggttttaag gtttcatcaa agctgacggt gaactgccgt
    17941 gggccaaaat ccagcagcag cgaaccgtta tcatccagac cgagatccgg tgctaaccga
    18001 tcttcaagct ccgccaccgt gagttcgcgg ctctcggcaa tgtcggcaat tttttctttt
    18061 gcccgctcct gtaatgcttt gaatttcagt ttctgggcga tgccgttaag ctgcataagg
    18121 gcgatatcac taccgatagc agcgagaata tccaacccaa cggtggcgcg tttatgctgg
    18181 gattcaccag gccaggcgcg tattaatggc gtcagtttgc gggcggtgtc atcgttaccg
    18241 agaacgccta acgcagtgaa cgcccaactc tctttcgacg gcgcgccagc ggtctgccag
    18301 gcggtaaaca gatcccaggc aaatcccgcc agtgagtcgg cggaacagac gtctttcacc
    18361 tgcaataatc ccggatacag agcctcttcc tgagggaagc ggagcatttc accgaggtgg
    18421 aggagggcgc tatctggcag tgattgggca tttgctttta ataccgggcg cgtccagagc
    18481 gatggctgat aaaaggccgg aagagtgggg attttgtcgg gtgattatct aagggatcga
    18541 gcgcaagcag agcgttcacc gcatcggtta cttccggctg gttataacgt cgggcgattt
    18601 cttgcagtaa tggctgatga ccgttttcgg taagcatacg caaggcagcg cgggcgttat
    18661 cctgtgcttc accggctttg ccgagcgccg caggcagcag gccggtaagg gcatgttccg
    18721 ggtatttcaa cagccagcta cgggcgtttt cgcgtagcgt tttcagtttg ttgaaggcac
    18781 gggcgacggc aggagccagc tcactcgctg cgaagtaatt cgtgatgggc aaggcttctt
    18841 gtggatagcg tgcaagtgag tttactaacc caggtaaccc tttgagtcca aaatgtaaca
    18901 tggcgtattc ggtatcggta tgtggagata gggtaagggc attccatgct tttactgctt
    18961 tctccagcgg caatgttgtc aaaatcccca actgccaata atgcccggta ggtgaatgaa
    19021 acaccttaaa ttcacttagt aaggttgaat aatcttcatt tagccatgcc tcaactaccg
    19081 cttcgggagc tttaatatat gtaccagcga tccgtcgttg aaaacccatc cttgccaaca
    19141 ggttggtagt actttcttca tggctaaccg tgatgtgttt tgaataccag atatatttcg
    19201 ccaaaagttg attactgatt tcttctgtca gataacagat tggctcaatg cctaatggcg
    19261 ctaaatccag caccggaatc ggcgattttt tctttttcga aagccaggga ggggagacta
    19321 ctacggctgg cagtagatcg gcgctggcat ggtttgaggg ctgtgtcagt tgttgctggc
    19381 atgatttcag tacggcaact gcgggtgtcg agagccaggg aatgacctgt tctgccagtg
    19441 ctggttgtga gataagcatt gtcattagca taatgcgcca actgttctct tctttttgcc
    19501 caagaagttc cgtcagtgcc gccattgctg catgtgggaa cgcagcgcag gctttcgtca
    19561 tccgatcgtg acagcgttta gtatgcccgg ctacacgtat aagcagtgtt aatgcgaacg
    19621 gatgattgat atgacgcaac acatcggcgc agtagtcact tgcggcatag ggagcaaatc
    19681 ggggcagggc tgcaacacct tgctcctgta atacggtggc tgaccatatc gcaccacaat
    19741 agtaatcatc aaaaatggca tatggctcgt gatatttttc taatgaggca agaatgtgct
    19801 catcagtcgc cactattttt aaccactcaa gcgagggtaa ttttttttgc cccagtagac
    19861 gctgggcaat ttcatgtgca atttctggtt tttcgggtaa taacaaagca attaatggtt
    19921 gccgccattc tggaatatgt ggaattgcgg caataagttt ttgtgcacat ttttgccaga
    19981 gagactcttc cgccagtgat aaatgtttac gtaggcgtaa ttcaaatctg gaatattgct
    20041 ccaagtagtc aggtgcaatg ccagacggca gaataataat gacattattc gcataatccc
    20101 atttaatatc aatctgttga aggtgtatta ttacatcagt agcatattcc agaccgcctt
    20161 cctggacgat ctgatccata acatcctcac gcactttata tcttgtgcat ttcatttcaa
    20221 gtgccgtgag tacagccatt actggtgatg gtagctgtgt tgttttcagg ccgtcgatct
    20281 gactgatcgc ctgggcgacg gtgttttgcc attctaaaga acaggcgctg aagtcaaatg
    20341 tggctgtttt attatcccgt tgcgacacac tacaggctaa gcgacgtaat tgggtcaatt
    20401 tgtctgcttg ctgaacagga actccgggaa agcggcgatg acttaacgtg gtgtgtacaa
    20461 cttcgggcgg aaaaacgaca gcaatatctt tcgccagcca cggtaaaaca gcatctgtat
    20521 ttttgtcagg cgcacgggtt tctgctacga aagagggttg ttcctgaata atggtggata
    20581 agtccgctac cttgagagag cccgttaccg tttgggaagg ttgcaaagaa ttatccttcg
    20641 cttgctccac ataccccttc ttcaccttct ccgcaatcag cttaagctcc gcttttgcca
    20701 ctgccgcagc atctgaaaaa ctttttatct ggctttgccc tttggtgcca acttttcccc
    20761 aactgatatg caactcgttt ccctgttgct caactgccca gaatttatgt gatttttcgt
    20821 cctgatagat aaagtgtctc attctgcatc cctgtaattt tatccagttg aatgcagatg
    20881 ctaccagtat ttatgcgggt tagagagaga caaaatcgca agaaaacaac tttaggaatt
    20941 tataaagaaa aggcgctgtc gatgcagcgc cttaaagggg gattatttca cctgatggcc
    21001 tggtttagca ccagcatccg ggcttaacag gaagatatct ttcccgccag gaccagcagc
    21061 cattaccatg ccttcggaga taccaaagcg cattttacgc ggtgccaggt tggccaccat
    21121 aatggtgtga cgcccgatca gtgcctgcgg atccgggtag gcagaacgaa taccggagaa
    21181 gacattgcgt ttttcaccgc cgagatccag tgtcaggcgc agcagtttgt cagaaccttc
    21241 aacaaactct gcgttttcga tcagcgccac gcgcaggtca acttttgcga agtcgtcaaa
    21301 ggtgatggtt tcctgaatcg gatcatctgc cagtgggcca gtaactggcg cggcagctgc
    21361 tttcacttct tctttagacg cttccaccag tgcttcaacc tgcttcatat cgatgcggtt
    21421 atacagcgct ttgaacggat tcactttgtg gcccagcagc ggttgctgga taccatccca
    21481 ggtcagttct gtattgagga atgcttctgc gcgctcggtc agtttcggca gtaccggctt
    21541 caggtaagtc atcagcacgc ggaacaggtt tatgcccatt gagcaaattg cctgcaggtc
    21601 ggcatcgcgg ccttcctgtt ttgccaccac ccacggagcc tgctcgtcga catagcggtt
    21661 agccagatca gccagcgcca tgatttcgcg cactgcttta ccaaattcgc ggctttccca
    21721 cgcttcacca atcacttcag cggcatcagt gaaggttttg tacaactgcg ggtcagccaa
    21781 ttcgcttgcc agcacgccgt caaaacgctt gttgataaag cccgcattac gagaggccag
    21841 gttaaccact ttgttcacga tatcggcatt cacacgctga acgaaatcct ccaggttgag
    21901 atcgatatca tcaatgcgcg aagagagttt cgcagtgtag tagtaacgca ggctgtcagc
    21961 gtcaaaatga ttcagccagg tgctggcttt aataaaggtg ccgcgagact tggacatctt
    22021 tgcgccgttc accgtcacat agccatgaac aaacaggttg gtcggcttgc ggaagttgct
    22081 gccttccagc atggcaggcc agaacaggct gtggaagtaa acaatatctt taccgatgaa
    22141 gtggtacagc tcggcggtgg agtctttctt ccagtattca tcgaagctta cgctgtcgcc
    22201 gcgcttgtcg cacagattct tgaaagaacc catgtagcca atcggtgcgt ccagccagac
    22261 gtagaaatat ttgcccggcg cgttcggaat ttcaaaacca aagtatggcg cgtcgcggga
    22321 gatatcccac tgttgcaggc cggattcaaa ccactcctgc attttatttg ccacctgctc
    22381 ctgcaacgca ccgctgcggg tccatgcctg caacatttcg ctgaaagagg gcagatcaaa
    22441 gaagaagtgt tcagaatcac gcattaccgg cgtagcgcca gaaaccaccg atttcggctc
    22501 gatcaattca gtcgggctgt aggtcgcgcc gcagacttcg cagttatcgc cgtattgatc
    22561 cggggattta catttcgggc aggtgccttt cacaaaacgg tccggcagga acatgccttt
    22621 ttccggatcg tacagctgag agatggtgcg gtttttaata aaaccgttct ctttcaggcg
    22681 agagtagata agttctgaca actggcggtt ctcttcgctg tgcgtcgagt gatagttgtc
    22741 atagctgatg ttaaagcctg cgaaatcagt ctgatgctcc tgactcattt cgccaatcat
    22801 ctgctccggg gtgataccaa gctgctgagc tttcagcatg atcggtgtac cgtgggcatc
    22861 gtcggcgcag atgaagttga cctcgtggcc gcgcattcgc tggtaacgga cccagacatc
    22921 agcctggatg tgctccagca tatggccgag gtggattgag ccgttagcgt acggcagtgc
    22981 gcacgtcacc agaattttct tcgcgacttg agtcatagta ggcattactt cttaatagtg
    23041 aaaaggggtt agatagtacc aaatgggaaa atgttaagta agtacttctc tatattcttc
    23101 aggtaaacta tcagctgatg gtttatctat acaaaaaagg agtcgggatg aacgaacaat
    23161 cccaggccaa atcaccagaa gccttgcgcg caatggtcgc cgggacactg gccaattttc
    23221 agcacccaac cctgaagcat aacctcacca cgctgaaagc gttgcaccat gtcgcctgga
    23281 tggacgacac gttgcatgtg gaactggtta tgccattcgt ctggcatagc gcgtttgaag
    23341 agttaaaaga gcaatgtagc gccgaactgc tgcgtatcac cggggcgaaa gctatcgact
    23401 ggaaactgtc gcacaatatc gccaccttaa aacgcgtgaa gaatcagccg ggtattaatg
    23461 gtgtgaagaa catcattgcg gtcagctccg gtaaaggcgg cgtgggtaaa tcctccacgg
    23521 cggtaaacct ggcgctggca ctggcggcgg aaggggcgaa agtcggcatt ctggacgccg
    23581 atatctacgg cccatcaatt cccaccatgc tgggcgcaga aaatcagcgt ccaacgtcgc
    23641 cggacggtac ccacatggca ccgattatgt ctcatggcct ggcaaccaat tcaattggtt
    23701 atctggtcac tgacgacaat gcgatggtgt ggcgtggacc gatggcgagc aaggcgttaa
    23761 tgcagatgtt gcaggaaacc ttgtggccgg atctcgacta tctggtgctt gatatgccgc
    23821 caggcaccgg tgacattcaa ttgacgctgg cgcaaaacat tccagtaact ggtgcagtcg
    23881 tggtcaccac cccgcaggac atcgcgttga tcgatgcgaa aaaaggcatt gtgatgttcg
    23941 agaaagtcga ggttccggtg cttggtattg tcgagaacat gagtgtgcat atttgcagta
    24001 actgtggtca ccatgaaccg attttcggta ccggtggagc tgagaaactg gctgaaaaat
    24061 accacacaca gctgctgggg cagatgccgt tacacatctc cttacgtgag gatctcgata
    24121 aaggcacgcc aacggttatc agccgtccgg agagcgaatt taccgctatc taccgccagc
    24181 tggctgaccg cgttgcagct cagctctact ggcagggtga agtcattcca ggcgagattt
    24241 ccttccgcgc ggtctaacgc ctattaacca ttctggttat tcttcataca ccgcagagat
    24301 gttacattga tgcggtgtat tagtttttgc cgcattaatg aacacttatt taatactgcg
    24361 aatattctgc ggaccttcgt ccgaaactct acaaagtcga ttgatcatac aggcaatgct
    24421 tcattatcat ccttcgtggc tgtgtagtgg atgtacagct atagtttgtt atgcactcat
    24481 tctatccagg cataaccttt actacaggga taacgttcaa tgaataagta ttggctgtca
    24541 ggaattattt ttcttgccta tggattggct tctcccgcat tctcttcaga gactgccaca
    24601 ttagcgatta atggcaggat cagtccccca acatgcagta tggctatggt taatggtcaa
    24661 cctcaacagc attgtggtca gttaacgtat aatgttgaca cccgtcatct gttttcttcg
    24721 cccgtcaaag gtgtaacgac agaagtggtt gttgcaggta gcgatagcaa acgtcgaatc
    24781 gtgttaaatc gctacgatta atttggcaac ctgattttaa ttgcatttag aaagccgaaa
    24841 tcatttat
//
LOCUS       DAZFQD010000069        24786 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.069, whole genome shotgun sequence.
ACCESSION   DAZFQD010000069 DAZFQD010000000
VERSION     DAZFQD010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24786
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.069"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            129..671
                     /gene="yfaZ"
                     /locus_tag="ACHKTW_002824"
     CDS             129..671
                     /gene="yfaZ"
                     /locus_tag="ACHKTW_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaZ family outer membrane protein"
                     /protein_id="HFO6463818.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIQSYEEANAGARYTIMRPVSIEAGYRYLNLSGK
                     DGNRDNAVADGPYVGVNASF"
     gene            771..1973
                     /gene="yfaY"
                     /locus_tag="ACHKTW_002825"
     CDS             771..1973
                     /gene="yfaY"
                     /locus_tag="ACHKTW_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HFO6463819.1"
                     /translation="MLKVEMLSTGDEVLHGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVQLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELILTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVVPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     IESMTLSV"
     gene            2037..2498
                     /locus_tag="ACHKTW_002826"
                     /pseudo
     CDS             2037..2498
                     /locus_tag="ACHKTW_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416748.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
     gene            complement(2539..3441)
                     /gene="rpnE"
                     /locus_tag="ACHKTW_002827"
     CDS             complement(2539..3441)
                     /gene="rpnE"
                     /locus_tag="ACHKTW_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HFO6463820.1"
                     /translation="MTESTTSSPHDAVFKTFMFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRLMRYATAA
                     MQRQLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLGRQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDRDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIEEIAERSPLQKERLMTIAERLRQEGHQIGWQEGMHEQAIKIALRMLE
                     QGIDRDQVLAATQLSEADLAANNH"
     gene            complement(3635..4825)
                     /gene="glpC"
                     /locus_tag="ACHKTW_002828"
     CDS             complement(3635..4825)
                     /gene="glpC"
                     /locus_tag="ACHKTW_002828"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit GlpC"
                     /protein_id="HFO6463821.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSTAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRKIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVITDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            complement(4822..6081)
                     /gene="glpB"
                     /locus_tag="ACHKTW_002829"
     CDS             complement(4822..6081)
                     /gene="glpB"
                     /locus_tag="ACHKTW_002829"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HFO6463822.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVTDIHSGLESLRQQAPAHPYSLLEPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRVTPLGTLRATWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLSVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDETLRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            complement(6071..7699)
                     /gene="glpA"
                     /locus_tag="ACHKTW_002830"
     CDS             complement(6071..7699)
                     /gene="glpA"
                     /locus_tag="ACHKTW_002830"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0046174 - polyol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HFO6463823.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            7972..9330
                     /gene="glpT"
                     /locus_tag="ACHKTW_002831"
     CDS             7972..9330
                     /gene="glpT"
                     /locus_tag="ACHKTW_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015169 - glycerol-3-phosphate
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HFO6463824.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFFYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            9335..10411
                     /gene="glpQ"
                     /locus_tag="ACHKTW_002832"
     CDS             9335..10411
                     /gene="glpQ"
                     /locus_tag="ACHKTW_002832"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFO6463825.1"
                     /translation="MKLKLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMDLNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTTDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            complement(10453..10692)
                     /locus_tag="ACHKTW_002833"
                     /pseudo
     CDS             complement(10453..10692)
                     /locus_tag="ACHKTW_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            10690..10794
                     /locus_tag="ACHKTW_002834"
                     /pseudo
     CDS             10690..10794
                     /locus_tag="ACHKTW_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311148.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            10874..11524
                     /gene="inaA"
                     /locus_tag="ACHKTW_002835"
     CDS             10874..11524
                     /gene="inaA"
                     /locus_tag="ACHKTW_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HFO6463826.1"
                     /translation="MAVSAKYDEFNHWWATEGDWVEEPNYRRNGMSGVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYAQHAVSPYSDEVRQAMLKAVALAFKKMHSINRQHGCCYVRHIY
                     VKTEGNAEAGFLDLEKSRRRLRRDKAINHDFRQLEKYLEPIPKADWEQVKAYYYAM"
     gene            complement(11578..11832)
                     /gene="yfaE"
                     /locus_tag="ACHKTW_002836"
     CDS             complement(11578..11832)
                     /gene="yfaE"
                     /locus_tag="ACHKTW_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HFO6463827.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEM"
     gene            complement(11832..12962)
                     /gene="nrdB"
                     /locus_tag="ACHKTW_002837"
     CDS             complement(11832..12962)
                     /gene="nrdB"
                     /locus_tag="ACHKTW_002837"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ia ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFO6463828.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDAEVDTDDLSNFQL"
     gene            complement(13109..15394)
                     /gene="nrdA"
                     /locus_tag="ACHKTW_002838"
     CDS             complement(13109..15394)
                     /gene="nrdA"
                     /locus_tag="ACHKTW_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1a ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFO6463829.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPAIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINSLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGVRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDTIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            15522..15665
                     /gene="ypaB"
                     /locus_tag="ACHKTW_002839"
     CDS             15522..15665
                     /gene="ypaB"
                     /locus_tag="ACHKTW_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001753443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpaB"
                     /protein_id="HFO6463830.1"
                     /translation="MALLQVHNFVDNSGRKKWLSRTLGQTRCPGKSMGREKFVKNNCS
                     AIS"
     gene            16090..19824
                     /gene="yfaL"
                     /locus_tag="ACHKTW_002840"
     CDS             16090..19824
                     /gene="yfaL"
                     /locus_tag="ACHKTW_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
                     /protein_id="HFO6463831.1"
                     /translation="MRIIFLRKEYLSLLPSMIASLFSANGFAAAIDLCQGYDIKASCH
                     ASRQSLSGITQDWSVADGQWLVFSDMTNNASGGAVFLQQGAEFSLLPENETGMTLFAN
                     NTVTGEYNNGGAIFAKENSTLNLTDVIFSGNVAGGYGGAIYSSGTNDTGAVDLRVTNA
                     MFRNNIANDGKGGAIYTINNDVYLSDVIFDNNQAYTSTSYSDGDGGAIDVTDNNSDSK
                     HPSGYTIINNTAFTNNTAEGYGGAIYTNSATAPYLIDISVDDSYSQNGGVLVDENNSA
                     AGYGDGPSTAAGGFMYLGLSEVTFDIADGKTLVIGNTENDGAVDSIAGTGLITKTGSG
                     DLVLNADNNDFTGEMQIENGEVTLGRSNSLMNVGDTHCQDDPQDCYGLTIGSIDKYQN
                     QAELNVGSTQQTFVHSLTGFQNGTLNIDAGGNVTVNQGSFAGTIEGAGQLTIAQNGSY
                     VLSGAQSMALTGDIVVDDGAVLSLEGDAADLAALQDDPQSIVLNGGVLDLSDFSTWQS
                     GTSYNDGLEVSGSSGTVIGSQDVVDLAGGDNLHIGGDGKDGVYVVVDASDGQVSLANN
                     NSYLGTTQIASGTLMVSDNSQLGDTHYNRQVIFTDKQQESVMEITANVDTRSTTTEHG
                     RDIEMRADGEVAVDAGVDTQWGALMADSSGQHQDEGSTFTKTGAGTLELTASGTTQSA
                     VRVEEGTLKGDVADIFPYASSLWVGDGATFVTGADQDIQSIDATSSGTIDISDGTVLR
                     LTGQDTSVALNASLFNGDGTLVNATDGVTLTGELNTNLETDSLTYLSDVTVNGNLTNT
                     SGAVSLQNGVAGDTLTVNGDYTGGGTLLLDSELNGDDSVSDQLVLNGNTAGNTTVVVN
                     SITGIGEPTSTGIKVVDFAADPTQFQNNAKFSLAGSGYVNMGAYDYTLVEDNNDWYLR
                     SQEVTPPLPPDPDPDPTPDPDPTPDPIPAYQPVLNAKVGGYLNNLRAANQAFMMERRD
                     HAGGNGQTLNLRVIGGRYHYTAAGQLAQHEDTSTVQLSGDLFSGRWGDDGEWMLGAVG
                     GYSDNQGESRSNMTGTRADNQNHGYAVGLTSSWYQHGNQKQGAWLDSWLQYAWFNNDV
                     SEQDDGTDHYHSSGIIASLEAGYQWLPGRGVVIEPQAQVIYQGVQQDDFTAANRARVS
                     QSQGDDIQMRLGLHSEWRTAVHVIPTLDLNYYHDPHSTEIEEDGSTISDDAVKQRGEI
                     KVGVTGNISQRVSLRGSVAWQKGSDDFAQMAGFLSMTVKW"
     gene            complement(19952..20674)
                     /gene="ubiG"
                     /locus_tag="ACHKTW_002841"
     CDS             complement(19952..20674)
                     /gene="ubiG"
                     /locus_tag="ACHKTW_002841"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HFO6463832.1"
                     /translation="MNAEKSPENHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     LTNTFKLGPGVDVNYMLHTQNK"
     gene            20821..23448
                     /gene="gyrA"
                     /locus_tag="ACHKTW_002842"
     CDS             20821..23448
                     /gene="gyrA"
                     /locus_tag="ACHKTW_002842"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HFO6463833.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVDGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            23597..>24786
                     /locus_tag="ACHKTW_002843"
     CDS             23597..>24786
                     /locus_tag="ACHKTW_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
                     /protein_id="HFO6463834.1"
                     /translation="MSGEKKAKGWRFYGLVGFGAIALLSAGVWALQYAGSGPEKTLSP
                     LVVHNNLQIDLNEPDLFLDSDSLSQLPKDLLTIPFLHDVLSEDFVFYYQNHADRLGIE
                     GSIRRIVYEHDLTLKDKLFSSLLDQPAQAALWHDKQGHLSHYMVLIQRSGLSKLLEPL
                     LFAATSDSQLSKTEISSIKINSETIPVYQLRYNGNNALMFATYQDKMLVFSSTDMLFK
                     DDQQDTEATAIAGDLLSGKKRWQASFGLEERTAGKTPVRQRIVVSARLLGFGYQRLMP
                     SFAGVRFEMSNDGWHSFLALNDESASVDASFDFTPVWNSMPAGASFCVAVPYSHGIAE
                     EMLSHISQENDKLTGALDGAAGLCWYEDSKLQTPLFVGQFDGTAEQAQLPGKLFTQNI
                     GAHE"
BASE COUNT         5915 a         6040 c         6771 g         6060 t
ORIGIN      
        1 ttatttaaga agtatttagt tacaataagt gactagatcc cttttttcgc tcattgaatc
       61 ctctgtgcca ggcagtacaa tcccgcagct ttgggaattt tgattaattg cttgtgggaa
      121 aggggataat gaaaaaaatt gcgctagcag gtctggccgg aatgctgttg gtttctgcat
      181 cggtcaatgc aatgagcatc agcggccagg cgggtaaaga atacaccaat attggtgtcg
      241 gttttgggac tgaatcgacg ggcctggctt taagcggtaa ctggacacat aacgacgacg
      301 acggtgacgt cgcgggcgtg gggctggggt tgaatctgcc tctcgggccg ttaatggcga
      361 ccgttggcgg aaaaggcgtt tacaccaacc cgaattacgg cgatgaaggt tatgccgcag
      421 cggtaggagg tggtttgcag tggaaaattg gcaacagctt ccgtttgttt ggcgagtatt
      481 actactctcc ggattcgctc tccagcggta ttcaaagtta tgaggaagcg aatgctggcg
      541 cgcgttacac cattatgcgt ccagtcagta ttgaggcggg ttatcgctac ctgaatctgt
      601 cgggtaaaga cggtaaccgc gacaacgccg tggctgacgg cccgtatgtt ggggttaacg
      661 ccagtttctg atcccttccg gcgcggcatt ctgtcgcgcc attcagtttc ttactacgcc
      721 ttatctccct ttgcgttata gtgattcgat aacaaaagca ggagataacc atgttaaaag
      781 tggaaatgct atccaccggg gatgaagtgt tacacgggca aatcgttgac actaacgctg
      841 cctggctggc cgattttttc tttcatcagg ggctgccatt atctcgccgc aatacggtgg
      901 gggataacct tgatgactta gtcaccattc ttcgcgaacg tagtcagcac gccgatgtgc
      961 tgatcgttaa cggcgggctg ggaccgacca gcgatgattt aagcgcactc gccgctgcga
     1021 cagcaaaagg tgaaggcctg gtgctgcatg aagcctggct caaagagatg gaacgctatt
     1081 tccacgaacg tggacgagta atggcaccga gcaaccgtaa acaagcggag ctgcctgcca
     1141 gtgctgaatt tatcaataac ccggtaggca ccgcctgtgg ttttgccgtg cagcttaatc
     1201 gttgcctgat gttctttact cccggcgtac cgtcagaatt taaggtgatg gtcgagcacg
     1261 aaatcctgcc gcgtctgcgc gagcgttttt ctttaccaca gccgccggtt tgtctgcgtt
     1321 taactacctt tggtcgttcg gaaagcgatc tggcacaaag cctggacact ctacaactgc
     1381 cgccgggcgt aacaatgggc tatcgctctt caatgccgat tatcgaactg atactcaccg
     1441 gaccggcaag cgagcaacag gcgatggaaa aactgtggct ggacgttaaa cgtgttgccg
     1501 gacagagcgt gattttcgaa ggcaccgaag gactgcccgc gcagatcagt cgcgaattgc
     1561 aaaaccgcca gttcagcctg acgttgagcg agcaattcac cggtggttta ttggctttgc
     1621 aactttctcg cgcaggtgct ccattgctgg cgtgtgaagt ggttccttca caggaagaaa
     1681 ccctggcgca aactgcgcac tggattacag aacggcgggc caaccatttt gccgggctgg
     1741 cactggctgt ttcgggtttc gagaacgaac atctcaactt tgcgctagcc acaccagacg
     1801 gcactttcgc tctgcgtgtg cgtttcagca ctacgcgcta cagcctggct atccgtcagg
     1861 aagtgtgcgc aatgatggca ctgaatatgc tgcgccgttg gttaaacggc caggatatcg
     1921 ccagtgagca tggctggatt gaggttattg agtccatgac cttatctgtc tgattccgcc
     1981 actctgtgcc ggtttactgc accggcacat tccccctccg gcaaaacttg tccaaccctc
     2041 cctacggtga gcgtggtgtt ggggccagtg tggcacgggc tgcgcgctgg ggacgcattg
     2101 agaattacat ggcgcaagtt aacgattcac tttgtctgtt ggtgcaggtg gaaagtaaaa
     2161 cggcactgga taacctggac gagatcctcg acgtcgaagg gattgatggc gtgtttattg
     2221 gaccagcgga tctttctgcg tcgttgggct acccggataa cgccgggcaa ccggaagtgc
     2281 agcgaattat tgaaaccagc attcggcgga tccgtgctgc gggtaaagcg gctggttttc
     2341 tggctgtggc tcctgatatg gcgcagcaat gcctggcgtg gggagcgaac tttgtcgctg
     2401 ttggcgttga cacgatgctc tacagcgatg ccctggatca acgactggcg atgtttaaat
     2461 caggcaaaaa tgggccacgc ataaaaggta gttattgata tcaaaggccc atggggatcg
     2521 gctgtgggcc tgtgttaatt agtggttatt cgctgccaga tcggcttcgc ttagctgggt
     2581 ggccgcgagc acctggtcac gatcaatgcc ctgttccagc atgcgcaaag caattttaat
     2641 ggcttgttca tgcatacctt cctgccagcc aatttgatgc ccttcctgcc gtagccgttc
     2701 agcaatagtc attaatctct ccttttgtag tggtgaacgt tcggcaatct cctcaataaa
     2761 acgggtgaaa cgggaggtat cgccgcattg cagcagataa ttaaacagtg tttgtagctg
     2821 gctgtcatta gtgaagcctc taaccaaaag cgtggtgatc ctgtcaacca tgccgattaa
     2881 atcgcggtcg cgaatatgct tttgaatcag ttccagcaga gctatacgcc gatgttgcat
     2941 gatctcatcg tcaggtacga tggtgatatc caccaacgga aaagcttcgg tgtacaactg
     3001 ccggccaagt tgcggatcgt caaactcatc cagccagtta agtgagtacg ggtagggcga
     3061 tgtttcgcca tgataaaaca gcaatggcac caccagcgga actctgtcat agcctttatc
     3121 cagctgacgc tgcatggcgg cagtggcata gcgcattagc cgaaaagcca tattcttttc
     3181 tgcagagctt tgatgttcaa tcacgcagta gatatagccg tcaccgtcgc tggtttccac
     3241 ggaccacaaa acatccgagt agtaagcgcg taaacttttt tcaataaaac tagtgggttc
     3301 caggcgtaag gtttgcaggt tgcaaagctt gcgcagtggt tctggtaaat gtatttcgag
     3361 aaaatcccgt gcggtttcgg gtgtgaacat aaaggtttta aacaccgcat catgcgggga
     3421 ggatgttgtt gattcggtca tggttgtccg tcacctgata tttatgatga cgcgacatta
     3481 cgtctatccc gggcctttga catctgtcag tttcagcctt tgcgagacat tttcagagga
     3541 attttcaaag cgttgcaacg catacatggt gcgtggaatc gcgctctcaa aaatgcccga
     3601 taagcaatgt gcttaccggg catcaaaagg tcggttaagc cagcgcctgg gccagtagcg
     3661 taatcggatg ttcgcagcga aggctcgtgg acatctcaat ctgccatttg caggtttcgc
     3721 agtcggtgat gaccagatcc gcgccgctct cttctatctg gcggaacagt ggcgcaccga
     3781 tggcttgtga ggtggggtag ttctcttttt tgaaaccgta agtacccgca ataccgcagc
     3841 actgggaatc cagcaccgtt aactcaagcc ccgggatttt acgcaacagc tccagggtgt
     3901 agagcgtcca gcccattttt tccatatggc acggagtgtg ataaaccact ttcagcggca
     3961 gcggtttcag cggtaacgtt ttgccttcgt ccagcttgcg ccacagccag cgggttgcca
     4021 gttcgatatg atcgcgcaag cctttgttgt cgacattcag cacttccggg tattcgtcgc
     4081 gcagggcaaa tgtacaggtt gaggaggtgg caatcaccgg aatgcctttt actcccacag
     4141 cttcgcggat cgactctaca ttcgtaattg cctgtttgcg tgctttatcg gtaaagccgt
     4201 tggcgattag cggtacgccg cagcattttt ctttgctgag cagttgtaca ccggtaccca
     4261 ttgcgttgag cactttaatt aaatctttac ctaactgcgg atggttgtag ttaacgaagc
     4321 agccgtgaaa gaaagcgacc tggtctttat attgtgcttg ctgagccgct gtgctgcgat
     4381 accagcgacg gaacgtgccg aaggagtatt tcggtagcgt gcggcgatga tcgattttta
     4441 acgccgcatc aagcagctgc cgcaccggtt tcagcgaggt agcggtgttg acgattggtg
     4501 cgaacggcgt ggaaacgcta cccatcaggt cggtatgact caacacaaaa ttacgcagcg
     4561 acgggcgcgt ggtgtcatat tttgcccgcg cgcgctggat aatatcgcca atcttcacat
     4621 cggacgggca ggcgacttca caacgtttgc agttgatgca atatttcagc gcctcgtcat
     4681 acagtgcgcc atctttcaaa cgcagacgct cgccatccgg cccggcttgt tttggccctg
     4741 gataaccggg attcacccgg ctcaccgggc aggcggtggt gcagacggtg cacttaatgc
     4801 agttttcgaa gctggtgtcg ttcattgttg gcctcctgcg cgttgggcaa tctgttgagc
     4861 ggcatgtaaa gcactgacgg cacaaacacc gccgccgcat ccctgggcga tgggatcaaa
     4921 tccgcccagc accgaaccga tggcaaacag gttctcaatg gtttgccctg cctgcgacgg
     4981 gcgtagcgtc tcatcagtgg ttacaccgaa ctgctgccac ggttgcggcg caaaaaaatc
     5041 gcccttatac cattcacccc gcgtggcggt ttgtagcaca tcaaggccga gaatcggctc
     5101 gcgaatgccg ttacgttccg ctaccagtcc gccactaaag aaactgccgc tggcgagaac
     5161 cgcgaaacgt ggacgtagcg gaatatcggc gtgattgcgg gtccagattt cattcactac
     5221 gccattttta caggtcactt ttttcacttc atcgcccggc atccacacgc caccctggcg
     5281 cacaaactgg cgctgtaact ggttttgcag acgaatgccc agcacggaag gcggcagcgt
     5341 tggcaaaagc atcagtgaac aaggtagttt ttcattcaac caacgccaca gtttgtcatc
     5401 ggccaggccg aagcaggcgg gcatcaggat catttcgcag gtattggcga caggaataag
     5461 cgcatcaagt aacagcggcc agttttcttc attatcaagg aaacgggcga tattcaccgc
     5521 gcgaaattcg gtggcgttat tgcgcaggac atccagttcc ggcagctcta tttctgcggt
     5581 ttcaaccgaa agatccagtt cgcgcaacga agccgccgca agatgcgcct ggaaatccat
     5641 cagcccgctt atccccacta cacatatctt cttcgcgggt agcggccaga cgggaacttc
     5701 tggcgaactt aaccaggttg cacgcaaagt gccgagcggg gtaacgcgct gatgggccag
     5761 ttccacgctg ccctgcaatt gcgcaccgct ttcagcgatc aatgcctgcg cctggcaagc
     5821 gagatcgagc acgcgttgtg gttcgagaag actgtaagga tgggctggtg cctgctgacg
     5881 caaagattcc agtccactgt gaatgtctgt caccggttga ccatcaggca gatggctcag
     5941 caaatccagc gaaccggatg agaaatgcag cgcgctttga ccacgcgtga caatggcaca
     6001 gcgcaggccg tgtttttgca gttgcaggcc acagagtaat ccggcgaggc cgccgcccat
     6061 aatgacagta tcaaagcgca tctttctgct ccttctccag accacacaat ccctgataaa
     6121 cccagcgggt aaattcgctt tcgcgcagtg catctcccca ggcgatgggt tgcacgcctt
     6181 tccagcgttc gttaaggaag gtggaaagtt gctcgataga ttgcgcggac gtcgtgacgt
     6241 taaaacgttg cagcagtccg gcagcgcggc aggcgcagag ttcgccctgg caggtaccca
     6301 tccccacacg ggtacgacgg cgtaaatcca gcaggctatt aacgtttaaa ttttctaccg
     6361 cgtactgaac ttcacccgca gttaccgctt cgcactcaca taccaggcta cgatgcagac
     6421 ggccttcgct cagccaggca ggcgtgcgat cgccatgacg ataaaccgca gaaccgcgca
     6481 gcggggcagg cagggagatg actttacgca aggtaacttc agcgggttct tgtgaaccag
     6541 gcagtgccag atcggcagtc gtacaggggc gcgtgttgcc cagtttgcgg cataccgcgt
     6601 cggtagccca ttcagccatc agccgatagg tcatcagttt gccaccggtg atggtgataa
     6661 atccgtccag accatcgcgt tcagcatggt cgagcagcac gatgccacgg ctgacgttac
     6721 gtccgctcgg gtcgtcatcg ctggcaacca gcgggcgcac gccagaatag gcccgcaaaa
     6781 tgcgcgtttt cgccatcacg ggggccagtt tttccccttc acgcagcaga atatcaacct
     6841 cttctgccgt cactcgatta tcgtcaatct cgttgtaatc aatacgtaaa gaggtggtgc
     6901 caatcagcga aatggtatcg ccaggcacca gaatatcggc gtcggaaggt ttacggcagc
     6961 ggttgatcac atgctggtta atgcggtgat ccatgatcag cagcgatcct ttcgccggga
     7021 acatgcgaat gcgcagatcg gcatattctg caatgtgttg cccccagatc ccagcggcat
     7081 taaccacgac aggtgcatga agggcctgag tttcgccggt gagatgatta cgtacacgaa
     7141 caccgcacac cgtcgcgcct tcacgaatca gccccgtgac ttcatgagcg gtaaggataa
     7201 cggcaccgtg ttctttggca tccagcatgt ttgctgcggt cagacgaaat ggatcaacgg
     7261 tgccatccgg aactttcacc gcgccaatca gtgccgggtt aacggcaggt tcgataatgc
     7321 gcgcttgctg cgggtctata gcttctgcgc tgatccctgc ttcttcgcag gcgcgaataa
     7381 aagtggcctg gaatgagagg tcatcttccg gcagggtgat aaacaggccg ttggttggtt
     7441 caacgcagtg acgtgcaatg cgtttcagga tctggttttc actaatgcat tcgcgggccg
     7501 attccgcatc ggttaccgca tagcgcgcac cgctgtgcag caggccgtgg ttacgcccgg
     7561 tggcaccggt tgcgatgtcg tggcgctcaa ccaaaatcac gcgcagcccg cgcagggcac
     7621 agtcgcgggc aatcccggct cccgttgcgc cgccaccaat gataatcacg tcacttgatt
     7681 gcgagtcgcg agttttcatt gttatccctc tgaagttcgt tttttaccat ttagccatag
     7741 taaaaacatg aattgtttga tttcgcgcat attcgctcat aattcgaaag tgaaacgtga
     7801 tttcatgcgt cattctgaac attttgtaaa tcttatttaa taatgtgtgc ggcaattcac
     7861 atttaatttc tgaatgtttt cttaacatcg cggcaactaa agaaacggca ggttctccca
     7921 ctgaatcagt ctgttaatca taaataagac cacgggccac ggaggctttc aatgttgagt
     7981 atttttaaac cagcgccaca caaagcgcgc ttacctgctg cggagatcga tccgacttat
     8041 cgtcgattgc gctggcaaat tttcctgggg atattctttg gctatgcggc ttactatttg
     8101 gttcgtaaga actttgcgct tgctatgcct tatctggtcg agcagggatt ctctcgcggt
     8161 gatttaggtt tcgcgctttc ggggatctcg atcgcttatg gattttcgaa attcataatg
     8221 ggttcggtat cggatcgctc gaatccgcgc gttttcctgc ccgcaggttt gattctggcg
     8281 gcggcagtga tgttgtttat gggctttgtg ccatgggcga cgtcgagcat tgcggtgatg
     8341 tttgtactgt tgttcctctg cggttggttc caggggatgg ggtggccgcc gtgtggtcgt
     8401 actatggtgc actggtggtc gcagaaagaa cgtggcggca ttgtgtcagt gtggaactgt
     8461 gcgcacaacg tcggtggtgg tattccgccg ctgctgttcc tgctggggat ggcctggttc
     8521 aatgactggc atgcggcgct ctatatgcct gctttctgcg ccattctggt ggcattattc
     8581 gcctttgcga tgatgcgcga taccccgcaa tcctgtggct tgccgccgat cgaagagtac
     8641 aaaaatgatt atccggacga ctataacgaa aaagcggaac aggagctgac ggcgaagcaa
     8701 atcttcatgc agtacgtact gccgaacaaa ctgctgtggt atatcgccat cgccaacgtg
     8761 ttcgtttatc tgctgcgtta cggcatcctc gactggtcac cgacttacct gaaagaggtt
     8821 aaacatttcg cgctggataa atcttcctgg gcctacttct tctatgagta tgcgggtatt
     8881 ccgggcaccc tgctgtgcgg ctggatgtcg gataaagtct tccgtggcaa ccgtggggca
     8941 accggcgttt tctttatgac actggtgacc atcgcgacta tcgtttactg gatgaacccg
     9001 gcaggtaacc caaccgtcga tatgatttgt atgattgtta tcggcttcct gatctacggt
     9061 cctgtgatgc tgatcggtct gcatgcgctg gaactggcac cgaaaaaagc ggcaggtacg
     9121 gcagcgggct ttaccgggct gtttggttac ctgggcggtt cggtggcggc gagcgcgatt
     9181 gttggctaca ccgtggactt cttcggctgg gatggcggct ttatggtaat gattggcggc
     9241 agcattctgg cggttatctt gttgattgtt gtgatgattg gcgaaaaacg tcgccatgaa
     9301 caattactgc aaaaacgcaa cggaggctaa tggcatgaaa ttgaagctga aaaaccttag
     9361 catggcgatc atgatgagca ctatagtcat gggaagcagt gcaatggcgg cggacagcaa
     9421 cgaaaaaata gtcatcgccc atcgcggtgc cagtggatat ttgccggagc atacgctgcc
     9481 agcaaaagcg atggcgtatg cgcagggagc ggattatctg gaacaggatt tggtgatgac
     9541 caaagacgac catctggttg ttctgcatga ccattatctc gatcgtgtta ctgatgttgc
     9601 cgatcgtttc ccggatcggg cgcgcaaaga cggtcgttac tacgcgatag atttcacgct
     9661 ggatgaaatt aagtcgctga aatttaccga aggtttcgat attgaaaacg gtaaaaaagt
     9721 acagacttat ccggggcgtt tcccaatggg taagtccgac ttccgggtgc acacctttga
     9781 agaagagatt gaatttgttc aggggttaaa tcactctacc gggaaaaata tcggtatcta
     9841 tccagaaatt aaagcgccgt ggttccatca tcaggaaggg aaggatattg cggcaaaaac
     9901 gctggaagtg ctgaagaaat atggttacac cggtaaggac gacaaagttt atttgcaatg
     9961 ttttgatgct gatgagctga agcgtattaa gaatgagctg gaacccaaaa tgggcatgga
    10021 tctcaatctg gtacagctga ttgcctatac cgactggaat gaaacgcagc agaaacagcc
    10081 ggatggaagc tgggttaatt acaactacga ctggatgttt aagccgggtg ctatgaaaca
    10141 ggtggcggaa tatgccgacg gtatcgggcc ggattaccat atgttgattg aggaaacatc
    10201 gcagccgggt aatatcaaac tcactggcat ggtgcaagat gctcagcaga ataaactggt
    10261 agtgcatcct tataccgtgc ggtcagataa actgcctgaa tacactactg atgtgaatca
    10321 gttatatgat gctctgtata acaaagcggg tgtaaatggg ctgtttactg atttccctga
    10381 taaggcagta aaattcctta ataaagagta atgctactgc aggatggcgt tcatgcgcca
    10441 tcctgtctca catcaaattt ttccattcac cacgagttgt tccgtacacg tttgtagtaa
    10501 aaacagtgga ctgccttcta ccggcttttc tttagccaac aacgaaatct gtcgatagaa
    10561 ggttggttcg tgaggaacgg aacacaattc acctgcaatg cttttcagcg ttaactctgg
    10621 ttgtaaggcg atgcctaatc cctgtcgaat aaacttcatc gctgtatccg actgattaaa
    10681 ttcacatttt atctactggg cgcactatta gtggtagtgg ctggtggaat ctatgcgctg
    10741 caaatgaagg gtaaaagcgg tgtcacgcta gtagtggcaa aagaaattca ttaatacgac
    10801 acgtttcatt aagattttcc tcaggtaacc acctatagtc attgtcgtta ataaaaatga
    10861 caggcgatag ggtatggcag tttcagcaaa gtacgacgaa tttaatcact ggtgggcaac
    10921 agagggcgac tgggttgaag agcctaacta tcgccgtaat ggtatgagcg gggtgcaatg
    10981 tgtcgagcgc aacggcaaaa agctgtatgt aaagcgcatg acgcatcatc tgtttcattc
    11041 cgtacgttat ccgttcggcc gaccaacgat tgtccgtgag gttgcggtaa ttaaagaact
    11101 ggaacgggcc ggtgtcatcg tgccgaagat cgtttttggt gaagcggtga aaattgaggg
    11161 tgaatggcga gcgttgctgg tgactgaaga tatggcgggg ttcatcagca ttgctgactg
    11221 gtatgcccag catgcagtat cgccttattc tgacgaagta cggcaagcca tgttgaaagc
    11281 agtggcgctg gcgtttaaga aaatgcatag cattaatcgt cagcatggct gttgttatgt
    11341 tcgccatatc tatgtgaaaa cagaaggtaa tgcagaagct ggttttcttg acctggagaa
    11401 aagtcgtcgc cgcttgcgtc gcgataaagc gattaatcat gacttccgcc agctggaaaa
    11461 atatctggag ccaatcccca aagcggactg ggagcaggtg aaagcgtatt actacgccat
    11521 gtaatcgttg taggccagat aagacgcata agcgttgtat ctggcactac aaccaattca
    11581 catctcgatt tcaatatcgc cttttgcccg gcaacaacag ggcaaaattt cccccggctg
    11641 aataaaggct aacggttcgg caatccagtc aacctgacct gcaaccagac gggtgcgaca
    11701 ggagccgcag taaccttcgc gacactgata ctcaaccgcc acattgtggg attccagcgc
    11761 cgccagaagg gaagggtgtt catcctggca cagcagttgt gtgccagtga tgcgcagggt
    11821 aacgcgggcc atcagagttg gaagttactc aaatcgtcgg tgtccacttc cgcgtcaatc
    11881 tgcccgacca gataagaact gacttccact tcctgcggag caacctgcac gttatcagac
    11941 accagccagg tgttgatcca tgggatcggg ttggagcgcg tctggaacgg cagatccaaa
    12001 ccgactgcct gcatacggat attggtgatg tattcaacgt actggcagag aatatcttta
    12061 ttcagaccaa tcatcgaacc gtcacggaac agataatccg cccagtcttt ctcctgttga
    12121 gctgcctgaa caaacaggtc atagcactcc tgcttacact cttcggcaat ttccgccatc
    12181 tcaggatcgt ccgcgccgct gcgcagcaga ttcagcatat gctgggtgcc ggtcaggtgc
    12241 agggcttcgt cgcgggcaat caggcgaata attttggcgt tgccttccat caattcgcgt
    12301 tctgcaaatg cgaaggaaca agcaaagctg acgtagaaac gaatcgcttc cagcgcgtta
    12361 acgctcatca ggcagagata cagttttttc ttcagctcgc gcaggctaac ggtcacagtt
    12421 ttaccgttaa cggtgtgggt accttcgccc agcagatgcc agtagctggt catttcgatc
    12481 agctcatcgt aatagctgga gatcccttcc gcacgtttct ggatctgctc gttggtgacg
    12541 atatcgtcaa acacaacaga cggatcgtta acgatattac gaatgatatg agtataggaa
    12601 cgggaatgaa tcgtttctga gaacgcccag gtttcgaccc aggtctccag ttccgggatc
    12661 gagatcagcg gcagcagtgc cacgttcggg ctacggccct gaatggaatc cagcagcgtc
    12721 tgatatttca ggttgctgat aaagatgtgt ttttcgtgtt caggcagtgc ctggtaatcg
    12781 atacggtcgc gagaaacgtc aacttcttcc ggacgccaga agaaagagag ctgcttttcg
    12841 atcagctttt cgaagatgtc atatttttgc tgatcgtagc gagccacgtt gaccggctga
    12901 ccaaagaaca tcggttcttt gagctgatca tttttcgtct gtgaaaaggt ggtatatgcc
    12961 atgagtgtgt cctgttggga gtttaatgcc ggataaggca ttttacgccg catcctgtgc
    13021 ctgatgcgac gctgacgcgt cttatcaggc ctacaaatcc gagccgtagg ccggataagg
    13081 cgttcacgct gcatccggca tctcaatatc agatcttaca tgcgccgctt tcgcagccat
    13141 cgtcctggat tgacggtacc agatcgtcct gcgcgtcttc agcgccgtca cgggtgttct
    13201 gataatacag cgttttgacc ccgaatttgt aggcggtgag caggtctttc agcaactgct
    13261 gcatcggcac ttttcctgac gggaaacgtg acggatcgta gttggtgtta gcagagatcg
    13321 actgatcgat aaatttctgc atgataccta ccagttgcag gtagccatcg ttacccggca
    13381 tttcccacag cagctcatag gcgtcgtgca ggtgctcgta gtccggcacc acctggcgca
    13441 aaataccgtc tttcgacgct ttgatgctga cgtaaccgcg cggcggttca ataccgttag
    13501 tggcgttaga gatctgtgaa gaagtctctg acggcatcag ggcagaaagc gtggagttac
    13561 gcaggccgtg cgttttgatt gattcacgca atgcttccca gtcgtaatga agcggctcat
    13621 tagcgatggt atccagatct ttcttatagg tatcgatcgg caggatccct ttcgcgtaag
    13681 tggtttcgtt aaaccacggg cacgcgcctt gctcttttgc cagctcgtta gacgctttca
    13741 gcaggtaata ctgaatcgct tcgaaggttt tatgcgtcag gttgttggcg ctgccgtcgg
    13801 agtaacgcac accgtgcttc gccaggtagt aagcgaagtt gatcacacca atacccagcg
    13861 tacgacgacc catcgctcca cgtttggcgg ccgggatcgg gtaatcctga taatccagca
    13921 gcgcgtcaag tgcacgaacc gccagaattg ccagctcttc cagttcatcc aggctattaa
    13981 ttgcgcccag gttgaaagca gacagcgtac acagcgcgat ttcaccgttc tcgtcgttga
    14041 cgtcgttcag cggtttggtc ggcagggcta tctccaggca caggttagac tgacgcactg
    14101 gcgcgatggc cggatcaaac gggctatggg tattgcagtg gtcaacgttc tgaatataga
    14161 tacgaccggt agacgcacgt tcctgcatca tcagcgagaa cagctcaacg gctttcacac
    14221 gctgcttgcg gatgctgtcg tctttctcat atttggtata caggcgttca aactcttcct
    14281 gatcggcgaa gaatgcgtcg tacagccccg gtacgtcgga cgggctgaac agggtgatat
    14341 cttcaccttt cagcaggcgg gtatacatca gtttgttgat ctgtaccccg tagtccatat
    14401 ggcgcacgcg gttgccttcc acaccacggt tgtttttcaa caccagcagg ctttccactt
    14461 ccagatgcca catcgggtag aacagcgttg ccgcaccgcc gcgcacgccg ccctgagagc
    14521 aggatttcac cgctgtctgg aaatgtttgt agaacggaat acagccggta tggaacgctt
    14581 caccaccgcg aatcgggcta cccagcgcac gaatacgccc ggcgttgatg ccgatcccgg
    14641 cacgctggga aacgtatttc acaatcgcgc tggaggtagc gttgatggaa tccagactgt
    14701 caccgcactc gatcagtacg caggagctga actgacgagt cggggtacgc acgccggaca
    14761 tgattggcgt cggcagcgaa attttaaatg tggaaaccgc gtcgtaaaaa cgcttcacat
    14821 attgcaggcg cgtttcacgc gggtagttcg agaacaggca cgcggcaacc agaatataaa
    14881 ggaactgggc gctttcgtag atttcgccgg tcacgcggtt ctgtaccaga tatttgcctt
    14941 ccagctgctt aacggcagca taagagaagg tcatatcacg gtcgtgatcg ataaacgtgt
    15001 ccatctgctt gaactcttct tccgtgtagt cttccagcag atgattatcg tatttgccca
    15061 tctcgaccat tttcaccacg tggtcgtaca gcgcaggcgg ctcaaactgg ccgtaggctt
    15121 ttttacgcag gtggaagatc gccaggcgcg cggcgagata ctgataatcc ggcgcatcac
    15181 gggagatcag gtctgcggcc gccttgataa tggtttcgtg gatgtcagag gtcttgatac
    15241 cgtcataaaa ctgaatgtgg gagcgcagct cgacctggga aatcgaaacg ttatgcagtc
    15301 cttctgccgc ccaatccaga acgcgatgga ttttgtcgag attgatgcgc tctgtgctac
    15361 cgtcgcgctt tgtcaccagc agattctgat tcatgtatgt cgtacctgtt tttggaattc
    15421 tttccgcaat agtttcatgc agactacctg tagtaaggga agctgcttag aacactatat
    15481 atagggggta tgtttgggat tcactgcaag atagtgtgaa aatggccctc ttgcaagtgc
    15541 ataactttgt ggataactca ggaaggaaaa agtggctttc gcgcacctta ggtcagacaa
    15601 ggtgtccggg aaagtcaatg ggaagagaaa aatttgttaa aaataactgt tcggcgattt
    15661 cttgaacggc agaataggtt ggataaattg atatagatgg tttattaatc tttttgctaa
    15721 tgattgcaaa aattgatcta agcacgagtc atcacataat ctgatgtatc agttgtggtc
    15781 aatatataaa cgaatgtaag cgattgttga tgtcgaaatg gttgttttct attcggttat
    15841 tgttaaatgc gaagccattt tgttaattta ccttatatta taagaaaatt attcattact
    15901 ttaatttagt ttaaatataa ctggtatccc ttaaggttat taacttgaat tatgacgcac
    15961 tgatactatt catcaaataa taacataata gccattgcat cgggttggac aatttacaaa
    16021 agaaagataa cccatattgt ataatgcgtt taaatatgcc gtcttatata aaatgatggt
    16081 tagatgaata tgcggattat ctttctacgc aaggagtatt tatctctact cccgtcaatg
    16141 attgcatctc ttttctctgc taacggtttc gcggcggcca ttgatttatg ccagggatat
    16201 gatatcaaag cgagttgtca cgccagcagg caaagccttt caggcattac gcaggactgg
    16261 agtgtcgccg atgggcaatg gctggttttt tcggatatga ccaataacgc cagcggagga
    16321 gccgtatttt tgcaacaagg agcggaattt tcactattac cagaaaatga aactggaatg
    16381 actctgtttg ccaataacac cgttacagga gaatataata acggcggggc catatttgct
    16441 aaagaaaact caacgctgaa tcttactgat gttatttttt ctggtaacgt cgcaggcggc
    16501 tatggtggcg caatctattc ttctggtact aatgatactg gtgccgtcga tttacgtgtc
    16561 actaacgcca tgtttcgcaa taatatcgct aatgatggca aaggtggcgc aatttatacc
    16621 attaataatg acgtctattt aagtgatgtt atttttgata acaaccaggc atatacatca
    16681 acaagttaca gtgatggcga tggcggggca atcgatgtta ccgataataa tagcgacagc
    16741 aagcatcctt caggttatac gataataaat aacactgcct ttacaaataa cactgccgaa
    16801 ggttatggcg gggcgatata taccaatagc gcgacggctc cctatcttat tgatatttct
    16861 gttgatgaca gctacagcca gaacggcggc gtgttagtgg atgagaacaa cagcgcagca
    16921 ggctatggag atggtccttc cactgcggcg ggtggcttta tgtatctcgg cttaagtgaa
    16981 gttacctttg atattgccga cggaaaaacg ctggttattg gcaatacaga gaatgacgga
    17041 gctgttgact ctattgctgg taccgggttg atcaccaaaa caggttccgg cgatctggta
    17101 cttaatgcag ataacaatga ctttactggt gagatgcaga ttgaaaacgg tgaagttacc
    17161 ctgggccgca gcaactccct gatgaatgtc ggcgatacgc attgccagga cgatccgcaa
    17221 gactgctacg gtctgacgat agggagtatt gataagtacc agaatcaggc agagctaaac
    17281 gttggctcca cccaacaaac ctttgtgcac tcattgacgg gctttcagaa tggcacttta
    17341 aatatcgatg ctggtggcaa tgttactgtt aatcaaggca gttttgctgg caccatcgaa
    17401 ggtgctggtc agctcaccat tgcgcaaaac ggcagctacg tgctgtcggg ggcgcagtcg
    17461 atggcgctaa ccggcgatat agtcgttgat gatggtgcgg tgctttcgct ggaaggcgac
    17521 gcggcagatc tcgccgctct ccaggacgat ccgcagtcga tcgtgttaaa cggcggtgtg
    17581 ctcgatctct ctgatttctc cacctggcag agcggcacat catacaatga tggccttgaa
    17641 gtcagtggca gcagcggaac ggttatcggc agtcaggatg tggtagatct tgcaggtggc
    17701 gacaatttgc atatcggcgg cgacgggaaa gatggcgtct acgtggtggt cgatgcgagc
    17761 gacgggcagg taagtctggc aaacaataat agttatttgg gcacaacaca aatcgcctcc
    17821 ggtacgctga tggtgagcga caactcgcag cttggagata cccactataa ccgccaggtt
    17881 atctttaccg ataagcaaca agaaagcgtg atggagatta ctgccaatgt cgatactcgc
    17941 tctacgacga ctgagcatgg gcgtgatatt gaaatgcgcg ccgacggtga agtggcagtt
    18001 gatgcggggg tagacacgca gtggggcgca ctgatggctg acagcagcgg gcagcatcag
    18061 gatgagggta gcacatttac taaaacgggg gcgggtacgc tggagctgac cgccagcggt
    18121 acaacgcagt cggcagtacg tgtcgaagaa ggcaccctga aaggcgatgt tgcggatatc
    18181 tttccttatg cttcgtcact gtgggtcggt gatggggcga cgttcgttac tggcgcggat
    18241 caggatattc agtctatcga tgctacttcc agcggcacta tcgatatcag cgatggtacg
    18301 gttttgcgtc tgaccgggca ggatacttcc gtcgccctta atgcctcatt gtttaacggc
    18361 gatgggacgc tggtgaatgc caccgatggc gtgacgttga caggtgagct taataccaac
    18421 cttgaaactg acagcctgac ctatctttcc gacgtgacgg ttaatggcaa tctgaccaat
    18481 acgtccggtg cggttagcct gcaaaatggc gtcgctggcg atacgctgac ggtaaacggt
    18541 gattataccg gcggcggtac gctactgctc gatagcgaat taaacggcga tgactcggta
    18601 agcgatcaat tggtgttgaa cggtaatact gctggcaaca caactgtggt ggttaactcc
    18661 attacaggga ttggtgagcc gacatcgaca ggcattaaag tggttgattt cgcagccgat
    18721 cccacgcagt ttcaaaacaa tgcgaagttc agtctggcag gcagcggcta cgtcaatatg
    18781 ggagcatatg actacacgct ggtggaagat aacaacgact ggtatctgcg atcgcaagaa
    18841 gtaacgccac cattgccacc tgatccagac cctgacccaa cacccgatcc tgacccaaca
    18901 cccgatccaa tccccgctta ccagccggtg ctgaatgcca aagttggcgg ttatctcaat
    18961 aatctgcggg cggcaaatca ggcgtttatg atggagcgac gcgatcatgc aggtggcaat
    19021 ggtcagacgc tgaatttacg tgttatcggc ggacgttatc attacacggc ggcagggcaa
    19081 ctggctcagc atgaagacac ttctacggtg cagcttagcg gtgacctgtt tagcgggcgc
    19141 tggggcgatg atggcgagtg gatgcttggg gcggttggtg gctacagtga taatcagggc
    19201 gagagccgct cgaatatgac cggaactcgc gccgataacc agaaccacgg ttatgccgtt
    19261 gggctgacct caagctggta tcagcacggt aatcagaagc agggggcctg gctggatagc
    19321 tggctgcaat acgcgtggtt taacaatgat gtttccgaac aagacgatgg cacagatcat
    19381 taccactcgt cggggattat cgcctcgctg gaagccgggt atcagtggtt accggggcgt
    19441 ggtgtggtga ttgaaccgca ggcgcaggtg atttatcagg gcgtgcagca ggatgatttt
    19501 accgccgcta accgtgcgcg cgtgtcacaa tcgcagggtg atgatattca gatgcggctg
    19561 ggtttacaca gcgaatggcg taccgctgtt catgtcatac caacattaga tctgaattat
    19621 tatcacgatc cccattcgac ggaaattgaa gaggatggca gcactatcag tgacgatgcg
    19681 gtgaagcaac ggggtgaaat aaaagtggga gtcacgggca atatcagtca gcgagtgtcc
    19741 ctgcgcggca gcgtggcgtg gcagaaaggg agtgatgatt ttgcccagat ggcagggttt
    19801 ttgtcgatga cggtgaaatg gtaatcactg tctagtcggc acggttgccg gatgcggcgt
    19861 aaacgcctta tccggcctac ggggagcatt tgtaggcctg ataagacgcg gcaagcgtcg
    19921 catcaggcat tggctcggcc aaacaacaac ctcacttatt ctgcgtgtgc agcatatagt
    19981 tcacatccac gccggggccg agtttaaaag tattagtgag cgggttgtaa tgtagcccgg
    20041 ttatatgtcg ctctttaagg cttgtctgat ctacccagcc aagcagttct gccggtttaa
    20101 taaacttctt cacatcatgc gtacctttgg gcaccatgcg caaaatatat tccgcgccaa
    20161 ccaccgccat cagccatgac ttgccgttgc ggttaagcgt cgagaaaaag acatcgccgc
    20221 ctggtttcac cagttgcgca caggctctga ccactgactg cggatcgggg acgtgctcca
    20281 gcatctccat gcaggtcacc acatcatact gcccggcatg ttttgccgcg tgctcttcca
    20341 cggtttcctg cacataatcc acctgaatgc cgctttccag cgcgtgcagt tttgccacct
    20401 gcaatggctc aaagcccata tccagaccgg tcaccgtcgc gccttcgcgc gccatactct
    20461 cggccagaat gccgccgcca caaccgacat cgagcacctt tttgccaaat aaaccgccag
    20521 cacgctcggc aatatagccc agacgcagcg ggttaatgcg gtgcaacggt ttgaactcac
    20581 cttcaagatc ccaccagcgg gaggcgacgg cttcaaattt agcgatctct tcgtggtcta
    20641 cgttatggtt ttccggcgat ttttcggcat tcattggcac ttctactccg taattggcaa
    20701 gacaaacgag tatatcaggc attggatgtg aataaagcgt ataggtttac ctcaaactgc
    20761 gcggctgtgt tataatttgc gacctttgaa tccgggatac agtagaggga tagcggttag
    20821 atgagcgacc ttgcgagaga aattacaccg gtcaacattg aggaagagct gaagagctcc
    20881 tatctggatt atgcgatgtc ggtcattgtt ggccgtgcgc tgccagatgt ccgagatggc
    20941 ctgaagccgg tacaccgtcg cgtactttac gccatgaacg tactaggcaa tgactggaac
    21001 aaagcctata aaaaatctgc ccgtgtcgtt ggtgacgtaa tcggtaaata ccatccccat
    21061 ggtgactcgg cggtttatga cacgatcgtc cgtatggcgc agccattctc gctgcgttac
    21121 atgctggtag acggtcaggg taacttcggt tccatcgacg gcgactctgc ggcggcaatg
    21181 cgttatacgg aaatccgtct ggcgaaaatt gcccatgaac tgatggccga tctcgaaaaa
    21241 gagacggtcg atttcgttga taactatgac ggcacggaaa aaattcccga cgtcatgcca
    21301 accaaaattc ctaacctgct ggtgaacggt tcttccggta tcgccgtagg tatggcaacc
    21361 aacatcccgc cgcacaacct gacggaagtc atcaacggtt gtctggcgta tatcgatgat
    21421 gaagacatca gcattgaagg gctgatggaa cacatcccgg ggccggactt cccgacggcg
    21481 gcaatcatta acggtcgtcg cggtattgaa gaagcttacc gtaccggtcg cggcaaggtg
    21541 tatatccgcg ctcgcgcaga agtggaagtt gacgccaaaa ccggtcgtga aaccattatc
    21601 gtccacgaaa ttccgtatca ggtaaacaaa gcgcgcctga tcgagaagat tgcggaactg
    21661 gtaaaagaaa aacgcgtgga aggcatcagc gcgctgcgtg acgagtctga caaagacggt
    21721 atgcgcatcg tgattgaagt gaaacgcgat gcggtcggtg aagttgtgct caacaacctc
    21781 tactcccaga cccagttgca ggtttctttc ggtatcaaca tggtggcatt gcaccatggt
    21841 cagccgaaga tcatgaacct gaaagacatc atcgcggcgt ttgttcgtca ccgccgtgaa
    21901 gtggtgaccc gtcgtactat tttcgaactg cgtaaagctc gcgatcgtgc tcatatcctt
    21961 gaagcattag ccgtggcgct ggcgaatatc gacccgatca tcgaactgat ccgtcatgcg
    22021 ccgacgcctg cagaagcgaa aactgcgctg gttgctaatc cgtggcagct gggcaacgtt
    22081 gccgcgatgc tcgaacgtgc tggcgacgat gctgcgcgtc cggaatggct ggagccagag
    22141 ttcggcgtgc gtgatggtct gtactacctg accgaacagc aagctcaggc gattctggat
    22201 ctgcgtttgc agaaactgac cggccttgag cacgaaaaac tgctcgacga atacaaagag
    22261 ctgctggatc agatcgcgga actgttgcgt attcttggta gcgccgatcg tttgatggaa
    22321 gtgatccgtg aagagctgga gctggttcgt gaacagttcg gtgacaaacg tcgtactgaa
    22381 atcaccgcca acagcgcaga catcaacctg gaagatctga tcactcagga agatgtggta
    22441 gtgacgctct ctcaccaggg ctacgttaag tatcagccgc tttctgaata cgaagcgcag
    22501 cgtcgtggcg ggaaaggtaa atctgccgca cgtattaaag aagaagactt tatcgaccga
    22561 ctgctggtgg cgaacactca cgaccatatt ctgtgcttct ccagccgtgg tcgcgtctat
    22621 tcgatgaaag tttatcagtt gccggaagcc actcgtggcg cgcgcggtcg tccgatcgtc
    22681 aacctgctgc cgctggagca ggacgaacgt atcactgcga tcctgccagt gaccgagttt
    22741 gaagaaggcg tgaaagtctt catggcgacc gctaacggta ccgtgaagaa aactgtcctc
    22801 accgagttca accgtctgcg taccgccggt aaagtggcga tcaaactggt tgacggcgat
    22861 gagctgatcg gcgttgacct gaccagcggc gaagacgaag taatgctgtt ctccgctgaa
    22921 ggtaaagtgg tgcgctttaa agagtcttct gtccgtgcga tgggctgcaa caccaccggt
    22981 gttcgcggta ttcgcttagg tgaaggcgat aaagtcgtct ctctgatcgt gcctcgtggc
    23041 gatggcgcaa tcctcaccgc aacgcaaaac ggttacggta aacgtaccgc agtggcggaa
    23101 tacccaacca agtcgcgtgc gacgaaaggg gttatctcca ttaaggttac tgaacgtaat
    23161 ggtttagtgg ttggcgcggt acaggtagat gactgcgacc agatcatgat gatcaccgat
    23221 gccggtacgc tggtacgtac tcgcgtttcg gaaatcagca tcgtaggccg taacacccag
    23281 ggcgtgatcc tcatccgtac tgcggaagat gaaaacgtag tgggtctgca acgtgttgct
    23341 gaaccggttg acgaggaaga tctggatacc atcgatggca gtgccgcgga aggggacgat
    23401 gaaatcgctc cggaagtgga cgttgacgac gagccagaag aagaataatt ttacttcttc
    23461 atgccaaaag ggagctatct cccttgtttg aattgaaaag tccaggctgc aaagtctggg
    23521 cttttgtcgt attagggcac ggtaaagttt ggctgtgccc gtaaaaaatg gctggctata
    23581 cacaaggaat gtggcaatga gtggtgaaaa aaaggcgaaa ggctggcggt tctatggtct
    23641 tgtaggtttt ggcgcaatag cactgctttc cgctggcgtc tgggcgttgc aatatgctgg
    23701 cagtgggcca gaaaaaacgt tgtcgccgct ggtggtgcac aacaatctgc aaatcgatct
    23761 caatgagccg gacctctttc tcgacagcga ctctctgagc cagcttccca aagatctcct
    23821 caccattccg tttctccacg atgttctgag cgaagatttc gttttctatt atcagaatca
    23881 tgccgatcgt ctgggcattg aaggcagcat tcgtcgtatt gtctatgaac acgatctcac
    23941 gctgaaagat aagctctttt cgtcactctt agatcagccc gcgcaggcag cgctgtggca
    24001 cgataaacaa ggccatcttt cacattatat ggtgctgatc cagcgcagtg gtttaagcaa
    24061 actgctggag ccattgttgt ttgccgctac cagcgacagc cagttaagca aaacggaaat
    24121 cagtagcatc aagataaata gtgaaactat tcctgtttat cagttgcgct ataacggcaa
    24181 taacgccctg atgttcgcga cttatcagga caagatgctg gtgttttcca gcacggatat
    24241 gttgtttaaa gatgatcagc aggataccga agccacggcg atcgcaggtg atttgttgag
    24301 cggcaaaaaa cgctggcaag ccagcttcgg tctggaagag cgtactgccg gaaaaacacc
    24361 agtacgccag cgcatcgtgg tcagcgccag gttgctgggt tttggctacc agcgattaat
    24421 gccttctttt gccggagtgc gtttcgaaat gagcaacgac ggctggcata gctttctggc
    24481 gttaaatgat gaatccgcga gtgtggatgc cagtttcgat tttacgccgg tctggaacag
    24541 tatgcctgcc ggggccagct tctgtgtggc ggtgccgtat tcacacggta ttgccgaaga
    24601 gatgctttcg cacatcagcc aggaaaacga caagctgact ggggcgttag acggtgccgc
    24661 ggggctgtgc tggtatgaag actcaaaatt gcaaaccccg ctgtttgtcg gtcagtttga
    24721 tggcactgcc gaacaagcgc aattgccagg gaaactgttt acgcaaaata ttggtgcgca
    24781 cgaaag
//
LOCUS       DAZFQD010000070        24574 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.070, whole genome shotgun sequence.
ACCESSION   DAZFQD010000070 DAZFQD010000000
VERSION     DAZFQD010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24574
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.070"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            180..1838
                     /gene="ybjD"
                     /locus_tag="ACHKTW_002844"
     CDS             180..1838
                     /gene="ybjD"
                     /locus_tag="ACHKTW_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HFO6463835.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(1835..2791)
                     /gene="ybjX"
                     /locus_tag="ACHKTW_002845"
     CDS             complement(1835..2791)
                     /gene="ybjX"
                     /locus_tag="ACHKTW_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HFO6463836.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRRSFRQ
                     KFLLRSLIIPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            2942..4057
                     /gene="macA"
                     /locus_tag="ACHKTW_002846"
     CDS             2942..4057
                     /gene="macA"
                     /locus_tag="ACHKTW_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019898 - extrinsic component of membrane
                     [Evidence IEA];
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HFO6463837.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSQQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            4054..6000
                     /gene="macB"
                     /locus_tag="ACHKTW_002847"
     CDS             4054..6000
                     /gene="macB"
                     /locus_tag="ACHKTW_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HFO6463838.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGISLDIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDVATLDADALAQLRREHFGFIFQRYHLLSHLT
                     AEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTVSGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTAT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(6073..6297)
                     /gene="cspD"
                     /locus_tag="ACHKTW_002848"
     CDS             complement(6073..6297)
                     /gene="cspD"
                     /locus_tag="ACHKTW_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HFO6463839.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            6447..6521
                     /gene="yljB"
                     /locus_tag="ACHKTW_002849"
     CDS             6447..6521
                     /gene="yljB"
                     /locus_tag="ACHKTW_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YljB"
                     /protein_id="HFO6463840.1"
                     /translation="MNQKFEAVNAIDRNVTDVADANDR"
     gene            6620..6940
                     /gene="clpS"
                     /locus_tag="ACHKTW_002850"
     CDS             6620..6940
                     /gene="clpS"
                     /locus_tag="ACHKTW_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HFO6463841.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            6971..9247
                     /gene="clpA"
                     /locus_tag="ACHKTW_002851"
     CDS             6971..9247
                     /gene="clpA"
                     /locus_tag="ACHKTW_002851"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HFO6463842.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     NLGDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(9591..9678)
                     /locus_tag="ACHKTW_002852"
     tRNA            complement(9591..9678)
                     /locus_tag="ACHKTW_002852"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9642..9644),aa:Ser,seq:gga)
     gene            complement(9932..10150)
                     /gene="infA"
                     /locus_tag="ACHKTW_002853"
     CDS             complement(9932..10150)
                     /gene="infA"
                     /locus_tag="ACHKTW_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFO6463843.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(10435..11139)
                     /gene="aat"
                     /locus_tag="ACHKTW_002854"
     CDS             complement(10435..11139)
                     /gene="aat"
                     /locus_tag="ACHKTW_002854"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HFO6463844.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            complement(11181..12902)
                     /gene="cydC"
                     /locus_tag="ACHKTW_002855"
     CDS             complement(11181..12902)
                     /gene="cydC"
                     /locus_tag="ACHKTW_002855"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HFO6463845.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDF
                     TLAFTLGGIMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRISDLTDQKPEVTFPDPQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALSEIL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARALLHDAPLVLLDEPTEGLDAT
                     TESQILELLAEMMSEKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(12903..14669)
                     /gene="cydD"
                     /locus_tag="ACHKTW_002856"
     CDS             complement(12903..14669)
                     /gene="cydD"
                     /locus_tag="ACHKTW_002856"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0042883 - cysteine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HFO6463846.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEVELASTDPVTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAATLRDNVLLARPDA
                     SEHELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGQIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(14792..15757)
                     /gene="trxB"
                     /locus_tag="ACHKTW_002857"
     CDS             complement(14792..15757)
                     /gene="trxB"
                     /locus_tag="ACHKTW_002857"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFO6463847.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMERMHEHATKFETEIIFDHINKVDLQNRPFRLTGDSG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            16302..16796
                     /gene="lrp"
                     /locus_tag="ACHKTW_002858"
     CDS             16302..16796
                     /gene="lrp"
                     /locus_tag="ACHKTW_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HFO6463848.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            16931..20998
                     /gene="ftsK"
                     /locus_tag="ACHKTW_002859"
     CDS             16931..20998
                     /gene="ftsK"
                     /locus_tag="ACHKTW_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFO6463849.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EITYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNGAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQPQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPAQQPYYAPAPEQPVAGNAWQAEEQQSTFAPQSTYQ
                     TEQTYQQPAAQEPLYQQPQPVEQQPVVEPEPVVEETKPARPPLYYFEEVEEKRARERE
                     QLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVPPVEAAAAVSPLASGVKKATLATGAAA
                     TVAAPVFSLANSGGPRPQVKEGIGPQLPRPKRIRVPTRRELASYGIKLPSQRAAEEKA
                     REAQRNQYDSGDQYNDDEIDAMQQDELARQFAQTQQQRYGEQYQHDVPVNAEDADAAA
                     EAELARQFAQTQQQRYSGEQPAGANPFSLDDFEFSPMKALLDDGPHEPLFTPIVEPVQ
                     QPQQPVAPQQQYQQPQQPVAPQQQYQQPQQPVAPQPQYQQPQQQVAPQPQYQQPQQPV
                     APQPQYQQPQQPVAPQQQYQQPQQPVAPQPQYQQPQQPVAPQQQDTLLHPLLMRNGDS
                     RPLHKPTTPLPSLDLLTPPPSEVEPVDTFALEQMARLVEARLADFRIKADVVNYSPGP
                     VITRFELNLAPGVKAARISNLSRDLARSLSTVAVRVVEVIPGKPYVGLELPNKKRQTV
                     YLREVLDNAKFRDNPSPLTVVLGKDIAGEPVVADLAKMPHLLVAGTTGSGKSVGVNAM
                     ILSMLYKAQPEDVRFIMIDPKMLELSVYEGIPHLLTEVVTDMKDAANALRWCVNEMER
                     RYKLMSALGVRNLAGYNEKIAEADRMMRPIPDPYWKPGDSMDAQHPVLKKEPYIVVLV
                     DEFADLMMTVGKKVEELIARLAQKARAAGIHLVLATQRPSVDVITGLIKANIPTRIAF
                     TVSSKIDSRTILDQAGAESLLGMGDMLYSGPNSTLPVRVHGAFVRDQEVHAVVQDWKA
                     RGRPQYVDGITSDTESEGGAGGFDGAEELDPLFDQAVQFVTEKRKASISGVQRQFRIG
                     YNRAARIIEQMEAQGIVSEQGHNGNREVLAPPPFD"
     gene            21153..21764
                     /gene="lolA"
                     /locus_tag="ACHKTW_002860"
     CDS             21153..21764
                     /gene="lolA"
                     /locus_tag="ACHKTW_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HFO6463850.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            21775..23118
                     /gene="rarA"
                     /locus_tag="ACHKTW_002861"
                     /gene_synonym="mgsA"
     CDS             21775..23118
                     /gene="rarA"
                     /locus_tag="ACHKTW_002861"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HFO6463851.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLGTEDIEQVLTQAMEDKTRGYGGQDIVLPDETRRAIAELVNGDARRALNTLEMM
                     ADMAEVNDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            23209..24501
                     /gene="serS"
                     /locus_tag="ACHKTW_002862"
     CDS             23209..24501
                     /gene="serS"
                     /locus_tag="ACHKTW_002862"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFO6463852.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
BASE COUNT         6105 a         6088 c         6644 g         5737 t
ORIGIN      
        1 atgctaatta tttgattttg ttgtttttgc agacttatca gcaagaggga gtataacgcg
       61 attattcgct catttttcag acatttgcca tgcttaaatg tgatgtcatc acgtattagc
      121 aaggcctttc ccgttatact gccagcgtaa aggataagtc acatatttct ggaggggata
      181 tgattcttga gcgcgttgaa attgtgggtt ttcgcggtat caaccgtttg tcgttgatgc
      241 tcgaacaaaa caacgtcctg attggggaga acgcgtgggg taaatccagc ttgctggacg
      301 ccttaaccct gctgctatcg ccagaatcag atctctacca ttttgagcgc gacgatttct
      361 ggttcccgcc gggagatatc aacgggcgag aacatcatct gcatattatt ttgaccttcc
      421 gcgaatcgct gccaggccga catcgggttc gccgttatcg gccgctggaa gcgtgctgga
      481 cgccatgcac cgatggctat caccgtattt tttatcgtct ggaaggggag agtgcggaag
      541 acggcagcgt gatgacactg cgcagttttc tcgataaaga cggacatccg attgatgtcg
      601 aggatattaa cgatcaggca cgccatctgg tgcgtttaat gccggtgctg cgcttgcgtg
      661 atgcccgttt tatgcgccgt attcgtaacg gcacggtgcc aaatgttccc aatgtggaag
      721 tcaccgcgcg ccagctcgat ttcctcgccc gtgagttgtc ctcacatccg caaaatctct
      781 ctgatggaca gattcgtcag ggactttccg caatggtgca gttgcttgag cattatttct
      841 ctgagcaggg ggctggacag gcgcgatatc gtttaatgcg gcggcgggcc agcaatgagc
      901 aacgaagctg gcgctatctg gacatcatca accggatgat tgaccgacct ggagggcgct
      961 cgtatcgggt tattttgctc ggcctgtttg ctactttatt gcaggcaaaa ggcacattga
     1021 gactggataa agacgcgcgt ccgttattgc taattgaaga tccagaaacc cgcctgcatc
     1081 cgattatgct ttccgttgcc tggcatctgt tgaatctttt gcctttacag cgtattgcta
     1141 ccaccaactc tggcgagctg ctttcgttaa ctccagtgga acatgtttgc cgcctggtgc
     1201 gtgaatcttc tcgtgttgcc gcctggcgtc tggggccaag tggcttgagc accgaagaca
     1261 gccgccgcat ctcttttcac attcgtttta atcgtccgtc atcgctgttt gctcgctgct
     1321 ggttgctggt ggaaggggaa acggaaacct gggttatcaa tgaactggcg cgtcagtgcg
     1381 gacatcattt tgatgccgaa gggatcaagg tcattgagtt tgcccagtcc gggctaaagc
     1441 cactggttaa atttgcccgc cgaatgggga ttgaatggca tgtactggtc gatggcgatg
     1501 aagcagggaa gaaatatgcc gctacggtac gcagcctgtt gaataacgat cgggaagccg
     1561 aacgagaaca tttaacggcg ttaccggcgc tggatatgga acattttatg tatcgccagg
     1621 gattttccga tgtgttccac cgcgtggcgc aaatcccgga aaatgtaccg atgaatctgc
     1681 gcaaaattat ctcgaaagcg atccatcgct cttccaaacc cgatcttgcc attgaagtgg
     1741 caatggaggc cggacgtcgt ggtgtggact ccgtaccgac gctgctgaaa aaaatgttct
     1801 cacgcgtgct gtggctggcg cgcggtcgcg cggattaacc gcgaaacatc gtggccattt
     1861 gtggctgaat agcgtcgagc atctcatagc gccgacggta ttcagcccgt tttttactgg
     1921 cgatttcggc aatctctttt cgtgctatct gtgctggaag gcggtaatgg cgttcagcat
     1981 cacatacgcc gccaaccgat tcccagaaag cgttgtaatc ggcatggatc ttgccttctt
     2041 tatcacgata acgcaggctg cggtaaatat gcgtttcatt gctgacggca ataatctgct
     2101 ctacctgcaa acgttgggca aacagacagg ccgcttccat cacgaggcgt ttgggaaata
     2161 gcccgtggca ggctttcgtc gcattctgga tttcctgatg tggaatttcc cattttgcgc
     2221 cttgcagtcc gccaataaac atcgttcttt tcccctgata ttcacacagg gtaaacgtga
     2281 tctctgccag aggaatacct tcgctgttgc gaaacaggat tgtgctgtca ccttctttat
     2341 ccattgagat catcatggtc agctcaagcg tgaactgctc gccgtttttg ccttccagct
     2401 tcgccagttg cagcccgggg gtattcaaat ataagctgaa ttcttccgcc gacatacatc
     2461 cgcggagtaa cgcataatgg taacgtaacg cctccagcaa ttgcttacgg ctaagattcg
     2521 ccgcaaggta agggcgatgc agacgcacag gcagtcgcgg ttggcgcgtt aacaacacat
     2581 tgagattagg ccagtgggaa agttcgttca tccactcaac gcttaaacgc ggtataatca
     2641 acgagcgcag caaaaatttc tggcgaaaac tacggcgatg ccagaattta cccggccgac
     2701 actgtccacg tgccagacta agaaaaagtg acaggctgct gagagattca gatggcgtaa
     2761 aggtccgttc agttagctgc gacatattca tgaaatcaat ggttatacat gacgtcgatt
     2821 tcaccattgc gtatcttaac caaacatcaa tagtgtgatt actaacgtaa attttatggt
     2881 tttgttgata tttcgttgaa gttaatgacc cggattggca tatggagtat tcagaatatt
     2941 tatgaaaaag cggaaaaccg tgaagaagcg ttacgttatt gcgctggtga tagtcatcgc
     3001 cggactgatt acgttgtgga gaattcttaa cgcacccgtg ccgacttatc agacgctgat
     3061 tgtgcgcccc ggtgatttac agcaaagcgt gctggcgacc ggaaagctgg acgcgctgcg
     3121 taaggttgac gtgggcgcgc aggtcagcgg tcagttgaaa actctgtcgg tggcgattgg
     3181 cgataaagta aaaaaagacc agcttttagg ggttattgat cctgaacagg ctgaaaacca
     3241 gattaaggag gtcgaagcaa cgctgatgga gctacgtgcg cagcggcagc aggcggaagc
     3301 ggagctgaaa ctggcgcggg tgacgtattc ccgtcagcaa cgtctggcac aaacgcaggc
     3361 tgtttcacag caggatctcg acaccgccgc gacggagatg gctgtgaaac aggcgcaaat
     3421 tggcaccatt gacgcgcaaa tcaagcgcaa tcaggcttct ctcgatacgg ctaaaaccaa
     3481 tctcgattac actcgcatcg ttgccccgat ggccggggaa gtcacgcaaa tcaccactct
     3541 gcaaggccag acggtgattg ccgcacaaca agcaccgaac attctgacgc tggcagatat
     3601 gagcaccatg ctggtaaaag cgcaggtttc tgaagcggat gtaatccacc tgaagccggg
     3661 gcaaaaagcc tggtttacgg tgcttggcga tccactgacg cgctacgagg ggcaaatcaa
     3721 ggatgtacta ccgacgccgg aaaaggttaa cgacgctatt ttctattacg cccgttttga
     3781 agtccccaac cccaatggtt tgctgcggct ggatatgact gcgcaagtgc atattcagct
     3841 caccgatgtg aaaaatgtgc tgacgatccc tctgtcggcg ttaggcgatc cggttggcga
     3901 taatcgttat aaagtcaaat tgttgcgtaa tggtgaaaca cgcgagcgtg aagtgacgat
     3961 tggcgcacgt aacgataccg atgttgagat tgtcaaaggg cttgaagcgg gcgatgaagt
     4021 ggtgattggt gaggccaaac caggagctgc acaatgacgc ctttgctcga attaaaggat
     4081 attcgtcgca gctatcctgc cggtgatgag caggttgagg tgctgaaggg catcagcctc
     4141 gatatttatg cgggtgagat ggtcgcgatt gttggcgctt cgggttccgg taaatcgacc
     4201 ctgatgaata ttctcggctg tctggataag gccaccagcg gcacctatcg cgtcgccggt
     4261 caggatgttg ccacgctgga cgccgatgcg ctggcgcaac tgcgccgcga gcatttcggc
     4321 tttattttcc agcgttacca tttgctttcg catttaaccg ccgagcagaa cgttgaagta
     4381 cccgccgtct atgctggtct tgagcggaaa cagcgactgc ttcgtgccca ggagttgctg
     4441 caacggctgg ggctggaaga ccgtacagag tattatccgg cacagctttc gggtggtcag
     4501 caacagcgcg tcagcatcgc gcgggcattg atgaacggtg gtcaggtaat tcttgccgat
     4561 gaaccaaccg gcgcactgga cagccattct ggcgaagagg tgatggcgat cctgcatcag
     4621 ctgcgcgatc gtgggcatac ggtgattatc gtcacccacg atccgcaggt cgctgctcag
     4681 gccgagcggg tgatcgaaat tcgcgacggc gaaattgtgc gcaatcctcc cgccattgaa
     4741 aaagtgaatg ttgctggcgg gacggagcct gttgtcaaca cggtgtctgg ctggcggcag
     4801 tttgtcagcg gttttaacga ggcgctgacg atggcatggc gggcactggc agcgaataaa
     4861 atgcgtactt tactgaccat gctggggatt attatcggta ttgcgtcggt ggtttccatt
     4921 gtcgtggtgg gtgacgccgc caaacaaatg gtgctggcgg atattcgttc tataggtacg
     4981 aatactattg atgtctatcc cgggaaagat tttggcgatg acgatccgca atatcagcag
     5041 gcgctgaagt acgacgactt aatcgccatc caaaaacaac cgtgggtcgc ctcagccaca
     5101 cctgccgtct cgcaaaacct gcgcctgcgt tataacaatg ttgatgttgc tgccagtgcc
     5161 aatggcgtga gcggcgatta ttttaatgtc tatggcatga ccttcagtga aggaaacacc
     5221 tttaatcagg agcagctgaa cggtcgtgcg caggtcgtgg ttctcgacag taatactcgc
     5281 cgccagcttt tcccccataa agcagatgtg gttggcgagg tgattctggt cggcaatatg
     5341 cccgccagag tcattggtgt ggcggaagaa aaacagtcga tgtttggtag cagtaaagtg
     5401 ctgcgtgtct ggctacctta cagcacgatg tccgggcgag ttatgggcca gtcgtggctt
     5461 aactccatta ctgtcagggt aaaagaagga tttgacagcg ccgaggcgga acagcaactc
     5521 acgcgtttac tttcactgcg ccacggtaag aaggatttct ttacctggaa catggacggc
     5581 gtcttgaaaa ctgttgaaaa gaccacacgc actttacaac tgtttctgac gctggtggcg
     5641 gtgatttcgc tggtggtggg tggtattggt gtaatgaata ttatgctggt gtcagtgacc
     5701 gagcggacgc gggaaattgg cattcgcatg gctgtaggtg cgcgagcaag cgatgttttg
     5761 caacagttcc tgatcgaagc cgtactggtt tgcctggtcg gtggcgcgtt gggaataaca
     5821 ctgtcactgt taattgcttt caccttgcag cttttcttac ccggctggga gattggtttt
     5881 tcaccgttgg cactgctgct ggcgtttctc tgctcgacgg ccaccgggat tttatttggc
     5941 tggttacccg cacgaaatgc ggcacgactg gatccagtag atgctctggc acgagagtaa
     6001 tttttgagat aaaaatgcca gccgatcggg ctggcatttt gcctttagga tgtacacaat
     6061 gagacagaag agctatgcga ctgccgcttc tacttcgacg ggcacaataa cactggcgtg
     6121 attgcctttt ggcccctggt ggacatcaaa ctgaacggat tgtccagctt ttagcgttct
     6181 gtaaccatcc atctgaatgg tggaataatg agcgaaaata tcttcgccgc cgccttcagg
     6241 gcagatgaaa ccaaaccctt tggcattgtt gaaccactta acagtaccct tttccatgct
     6301 tcgacatcct tcgcaaatct tatacaagta agatggaata aaccggggtc agagaggggg
     6361 ctgttcaaaa cctcgccaac tctagaaata caatttagag aattagggcg agccgtcaag
     6421 catttgacag gggacagggg gcaggtatga atcaaaaatt tgaagcagtt aacgctattg
     6481 acaggaatgt gacagatgtc gctgatgcca acgatagatg atagttatct atcatgtgaa
     6541 gtagattggt caggcaaata agctcttgtc agcggcaggg cgttctgccg ataaccgtaa
     6601 ccgaagatga taactgacaa tgggtaaaac gaacgactgg ctggactttg atcaactggc
     6661 ggaagaaaaa gttcgcgacg cgctaaaacc gccatctatg tataaagtga tattagtcaa
     6721 tgatgattac actccgatgg agtttgttat tgacgtgtta caaaaattct tttcttatga
     6781 tgtagaacgt gcaacgcaat tgatgctcgc tgttcactac caggggaagg ccatttgcgg
     6841 agtctttacc gccgaggttg cagaaaccaa agtggcgatg gtgaacaagt acgcgaggga
     6901 gaatgagcat ccattgctgt gtacgctaga aaaagcctga atgcaggcat aaaaattggg
     6961 ggaggtgcct atgctcaatc aagaactgga actcagttta aatatggctt tcgccagagc
     7021 gcgcgagcac cgtcatgagt ttatgaccgt cgagcacttg ttactggcgc tgctcagtaa
     7081 cccatctgcc cgggaggcgc tggaagcgtg ttctgtggat ttggttgcgc tccgtcagga
     7141 actggaagcc tttattgaac aaaccacacc cgttctgcct gccagtgaag aggagcgcga
     7201 cacacagccg acgctgagtt ttcagcgtgt actgcaacgt gcggtcttcc atgtccagtc
     7261 ctccggtcgc aatgaggtaa ccggtgcaaa cgttctggtc gctatcttta gcgaacagga
     7321 gtcgcaggcg gcatatctgt tgcgtaaaca tgaagtcagc cgtctcgatg tggtgaactt
     7381 tatctctcat ggcacgcgta aagacgagcc gacacagtct tctgatcctg gcagccagcc
     7441 aaacagcgaa gaacaagctg gtggggagga acgtatggag aatttcacga cgaacctgaa
     7501 tcagcttgcg cgcgtgggcg gaatcgaccc actgattggt cgtgagaagg agctggagcg
     7561 tgctattcag gttctctgcc gtcgccgtaa aaacaacccg ctgctggtgg gggaatctgg
     7621 tgtcggtaaa accgcgattg cggaaggtct tgcctggcga attgttcagg gcgatgtgcc
     7681 ggaagtgatg gctgactgta cgatttactc tctcgatatc ggttctctgt tagcgggcac
     7741 taaatatcgc ggcgactttg aaaaacgttt taaagcgttg ctcaagcagc tggagcagga
     7801 cactaacagc atcctgttta ttgatgagat ccacaccatt atcggtgcgg gtgcagcgtc
     7861 tggtggccag gttgatgcgg ctaacctgat caaaccgttg ctctccagcg gtaaaattcg
     7921 cgtaattggt tcgacaacct atcaggagtt cagcaacatt ttcgagaaag accgtgctct
     7981 ggcgcgtcgc ttccagaaaa ttgatattac tgaaccgtcg atcgaagaaa ctgttcaaat
     8041 catcaatggc ctgaaaccga agtatgaagc gcaccacgac gtgcgttata ccgcaaaagc
     8101 ggtgcgtgca gcggtagagc tggcggtgaa atacattaac gatcgtcatc tgccggataa
     8161 agccattgac gttatcgacg aagcgggcgc tcgcgcacgc ctgatgccgg taagcaaacg
     8221 caagaaaacc gttaatgtgg cggatattga gtccgtggtg gcccgtattg cgcgcattcc
     8281 agagaagagt gtttctcaga gtgaccgcga taccctgaaa aacctcggcg atcgcctgaa
     8341 aatgctggtc ttcggtcagg ataaagccat tgaggcgctg actgaagcca ttaagatggc
     8401 gcgtgcaggt ttaggtcacg aacataaacc ggttggttcg ttcctgtttg ccggccctac
     8461 cggggtcggg aaaacagagg tgacggtaca gctttcgaaa gcgttgggca ttgagctgct
     8521 gcgctttgat atgtccgagt atatggaacg ccataccgtc agccgtctga ttggtgcgcc
     8581 tccgggatac gttggttttg atcagggcgg tttgctgact gatgcggtca tcaagcatcc
     8641 acatgcggtt ctgctgctgg acgaaatcga gaaagcgcac cctgacgtgt tcaatattct
     8701 gttgcaggtg atggacaacg gtacgctgac cgataacaac ggacgcaaag cggacttccg
     8761 taacgtggtg ctggtgatga ccaccaacgc cggggtacgt gaaactgagc gtaaatccat
     8821 tggtcttatc caccaggata acagcaccga tgcgatggaa gagatcaaga agatctttac
     8881 accggaattc cgtaaccgtc tcgacaacat tatctggttt gatcatctgt caaccgacgt
     8941 gatccatcag gtggtggata aattcatcgt cgagttgcag gttcagctgg atcagaaagg
     9001 tgtttctctg gaagtgagcc aggaagcgcg taactggctg gccgagaaag gttacgaccg
     9061 ggcaatgggc gcacgtccga tggcgcgtgt catccaggac aacctgaaaa aaccgctcgc
     9121 caacgaactg ctgtttggtt cgctggtgga cggcggtcag gtcaccgtcg cgctggataa
     9181 agagaaaaat gagctgactt acggattcca gagtgcacaa aagcacaagg cggaagcagc
     9241 gcattaatct gattgttagg taggttggtc aagtccgtaa tctcgaaaga ggttacggac
     9301 tttttgttta tggggtggag gaggttcaga cccttttttt aatgatgatg gtaagttgtt
     9361 gataattagt gctgcgggaa ggtaaggata aaaaagggtg ctgcaggaga atgggatggt
     9421 tttgctttat taacaacggg ctaaacgtgt agtatttgag ttcactgccg tacaggcagc
     9481 ttagaaatcg acggggtgcg gtaaaacctt tgcgaacgcg ttcactgccg tacagacaga
     9541 taaaatgcga aaaaaaagct cgcactttcg tacgagctct tctttaaata tggcggtgag
     9601 ggggggattc gaacccccga tacgttgccg tatacacact ttccaggcgt gctccttcag
     9661 ccactcggac acctcaccaa attgttttgc taccaaacct catgggtggc aacggggcgc
     9721 tactataggg agttggagta aaacggtcaa gaagaatttt aatgataatt attgtttgct
     9781 catactgtaa acaagttgtg cagtatatct acatcgagac aagttacgga cttatacttc
     9841 caaagtactt catacatatc acaaaataaa aaggccggtt aaaccgacct tttactcgtt
     9901 ctttctcttc gcccatcagg cggtaaaaca atcagcgact acggaagaca atgcggcctt
     9961 tgctcaggtc gtacggggtc agttcaacag tcactttgtc gcccgtcagg atgcggatgt
    10021 agtttttgcg cattttaccg gagatgtgtg cagtaaccac gtgaccgttt tctaactcta
    10081 cgcggaacat ggtattaggc aacgtttcaa gaacggtacc ttgcatttca atattgtctt
    10141 ctttggccat ctaatcctct ggggtatcac taccgtaatt tgaaccggca agataatgcc
    10201 gaagttctgt aaataagtaa agatttgcgc gctaaatcgc aacaaacagg ttcggcacat
    10261 tactccgaaa acacacggct aagccgcacc aaaagcgcaa cgtataaggg agcggtgaga
    10321 taaacgatgg gcgttacctg acgcgaaaaa ttccttatcg gcagcggggt aatgagcgta
    10381 accaactctg cgaccgcaat tataacactc tggggagaaa tgtgccgaaa acattcattc
    10441 ttgtggtgaa aacaagcatc gtggtaccca gaaattattc ggcaatcgtc cgaggcgcat
    10501 ttgattgaga taattaaggt aatcccggcg gggaatttcg caggcaccaa gcgatgctgt
    10561 gtgatcgtta aggacctggc agtcgataag cttaccgcca tgaccgataa attcctcaca
    10621 gaataccaga agcgccgttt tagacgcatt ttccatccgg ctgaacatgg actcgccaca
    10681 aaatagcgtt ccctgggcca cgccgtacat accgccgaca agctcatctt cacgccagac
    10741 ttcaatggag tgggcatggc cgagttcgtg aaggcgatgg taggcttcga ccacgccacg
    10801 cgtgatccag gttccttctt cgcgatcgct ggcacagcct tcaatgacct gaccaaaagc
    10861 gtaattcatc gtgacacgat agggcgagcg tttatgaaat cgcttcatac tacggctgat
    10921 atgcagtgat tctggccata gcaccgcgcg gggatcgggc gaccaccaga ggatggggtc
    10981 gcctggagaa aaccacggaa aaataccacg ctggtaagcc attaacaggc gcgcagggct
    11041 aagatcgccc ccaagtgcca gcaggccgtt aggctcacgt aatgcgcctt ccggggaagg
    11101 gaaggctatt gaatggcgag aaagctgaac caggcgcatg accgcaaaac tccacgcaag
    11161 tcggatcgtt caataatagc ttacaaaccc tgcttgaact ggtaataacg cccctgtctg
    11221 gcaagcagct ctgcgtgagt accttgctca ataatttgcc cgttgtccat cactattatt
    11281 tgttggaaac gagagagtcc gcgaagtcga tgggtgacca ttaacaccgt tttctcactc
    11341 atcatttctg caagcaattc aaggatctgg ctttcggttg tggcatctaa gccttcggta
    11401 ggttcatcca gcaacaccag tggcgcatca tgtaacagcg cacgggcgat agccagacgg
    11461 cgcagttcac caccggagag ctggcgtccg ccttcaccta accaactgtt gagacctgca
    11521 tcctcgagca gcttctccag accaacgcga cgcaagatct ccgacagagc ctcatcacta
    11581 ctgccaggcg aggcgagtaa cagattatca cgcagcgtgg cgctaaacag atgcactcgc
    11641 tgaggaacaa cgctgatggt ctgtcgtaga gccgcttcat tcaggctggc tatggggcta
    11701 tcgttaagca aaatctcgcc ctgttgcggg tcccatgcgc gggtcagcag ttgtaacagt
    11761 gttgatttgc cgcatccggt tcgcccgaga atcgctatat gttccccggc gtttacctga
    11821 agagaaatcc ctttaagtgc ctgttgggat tgctccggat aagtgaactg aacatcccgt
    11881 aatgtcagcg aaacgcgatc ggcaacacga gtttggggat cagggaaggt gacctccggt
    11941 ttttgatccg ttaagtcaga gatacgtacg gcagaggcaa tgacttgccc cagatgctga
    12001 aatgcacccg ttactggtgc cagtgcttca aacgcggcta acgcgcagaa gacaaacagg
    12061 gcaattaacg cgccgggttg agcattgccg ccaacgccgc cagacgccat ccacagcatc
    12121 aggatcaccg ctaacgcgcc aatgagcagc attatcgctt gcgacaatgc ggtcagttca
    12181 gattgacggc gttgcgcttc cagccattga atttctgtat tctctagttg cgtgcgataa
    12241 cgatcgctgg caccaaaaat ggtcagctca gcttgccctt gcagccaggc cgtcagttgt
    12301 tggcgatact gcccgcgaag atgagtcaga ttttgcccgg tgctttttcc cgcacgataa
    12361 aacagcggtg gcatcaggaa aagcgtcagt aacataatgc cgccaagcgt aaaggcgagg
    12421 gtgaaatcaa ggaaacttaa cccgattgtc accaccataa tcaccacaaa agcgcccacc
    12481 agcggcgaga taacgcgcag gtaaagatga tcgagcgtat caacatccgc caccacgcgg
    12541 ttgagcaatt cgccctgacg atagcgtgcc agtccggcag gggagagggg cagcaatttg
    12601 ctgaaggtgt aaatgcgcag atgctgcaac acgcggaaag tcgcgtcgtg acttaccaga
    12661 cgttcaaaat aacgcccggc agtacgggtg attgctgcgc cacgcacgcc cgcagcgggt
    12721 agcatatagt tgaagctgta cagtccggca acccccgcaa ccgctgaggc cgagaggaac
    12781 cagccggaaa gtgtcaacag accgatactg gcgagcagcg tgacaattgc cagcacgata
    12841 ccaagactta acatccattt atgacgttta tacagtgcca gatagggtag caaagcgcgc
    12901 atttaaatct cctcctgacg atgggccagt aatgtggcga atgggccacc agccacactt
    12961 aattccgcgt aacgtccttg ctcaataatc tgaccatcct gcattaccca aatgacatcc
    13021 cagtcagcaa gatcttctaa ctggtgggtg accattaacg ttgtctggcg cagagaggcg
    13081 gcattcagcg cctccattac gcgctgttca ctgtgagcat caaggctggc agcgggttca
    13141 tccaatagta atagcgagca gggatttagt aacgcacggg ccaccgccac gcgctgcgcc
    13201 tgccccacgg aaaggcgggc agcctggtcg ccaacaggcg tatcaacgcc ttgtggcagg
    13261 agcggtagaa actcgctgac ccaggcgtta tccagcgctg cttgtaattc atgttcgctg
    13321 gcatcaggtc gcgccagtag tacgttatcc cgtaatgttg ctgccggtaa ttgtggattt
    13381 tgcccaaccc aggagagatg tttacgccat gattctggtg ataaatcgcg taattctatc
    13441 ccgttgattc gtagcgatcc ctgatatgag agaaaaccag aaagcgcgtt caacagtgaa
    13501 cttttacctg aaccgctgcg accaaccaac acggcacgtt ggcctgctgg caaagtaaag
    13561 ttcagcggtc cggccagcgt tttaccttcc ggcgacgtga taaacagatc ctcggcctca
    13621 atggtcaccg gatcggtcga tgctaattcc acctcgccac gctgcggatg ggcgagcggg
    13681 gtttccataa acgttttcag actgtcagct gcaccaacag cctgggcttt agcatgataa
    13741 aacgtaccga gatcgcgtaa tggctggaaa aactctggcg caaggatcag ggccagaaaa
    13801 cccgcagcca gcgtcacgcc agtatcgtag tgaccaaaat ccagctcgcc gagataggaa
    13861 aaaccaaagt agaccgccac cagagcaatt gacagcgagg taaaaaattc gagaatgccg
    13921 gaggataaaa atgccagccg tagcacttcc attgtccgtt ggcggaaatc ttccgaagca
    13981 gaacgaatac tttcaatttc agcttcacca cgaccaaaaa tacgcaatgt ttccatgccg
    14041 cgcaggcgat cgaggaaatg cccacttaag cgagcaagag cgagaaagtt acgtcggtta
    14101 gcatcggcag cccccatccc aaccagcgcc ataaacaacg gaattaacgg tgcagtgccc
    14161 agcagaatga gcgccgcagc ccagttagag gggaagatcg ccaccacaat cagcaacggc
    14221 accgacactg ccagcgccat ttgcggcagg tagcgtgcat agtaatcatg catatcgtca
    14281 atttgctcga gtaccagcgt cgcccagctc cccgcaggtt taccctgaat ccacgctggc
    14341 cctgcttgtt gcagacggtc gagaacctga cggcggatgg caaagcggat atgctgcccg
    14401 gcgtgataac ccacccgttc gcgtaaccag accacccatg cgcgcagtac aaaggtcaga
    14461 accagtaacg taaagggaag cagcagagct tcacggggaa tattctccat aatcatatgt
    14521 tgcagaattc gcgccatgaa ccaggcctgg gcaatgatca atatgccgct cacaaagccc
    14581 agcagacgag aaatattcag ccaacgttgg gagatgacgc tttgctgttt taaccagcgg
    14641 gttaactctt tttgacggga tttattcatt gcacgcttag caggtgagtt atcagaatta
    14701 tttgtagagc aatgttacaa cgaggaaaaa ataaaggcga cccatagtcg catggtgtcg
    14761 ccttctttac ttttgttact gatttgtaaa attattttgc gtcagctaaa ccatcgaggt
    14821 agcgttccgc atcaagtgct gccatgcagc ctgtaccagc agatgtaata gcctggcgat
    14881 aaatgtgatc catcacgtcg cctgcggcaa agacgccagg gatgctggtc tgggtggcat
    14941 taccatgaat acccgactgt actttgatgt agccgttttc cagttccagc tgcccttcga
    15001 aaatggcagt attcgggctg tgaccgatag caacaaacag accggcaacg tcgagtgact
    15061 cgatgttatc gctgttttgc gtatcgcgca gacgaacgcc agtgacgccc atctgatcgc
    15121 cggtcacctc ttccagcgta cggttggtgt gtagaatgat gttgccgttc tccactttat
    15181 ccatcagacg cttaataagg attttttccg cgcggaaacc gtcacggcgg tgaatcagat
    15241 gcacttccga agcgatgtta gacagataca gcgcctcttc aaccgcggta ttgccgccgc
    15301 cgatgaccgc aactttctgg ttgcgataga agaaaccgtc gcaggtagca caagcagaaa
    15361 ctccacggcc tttaaaggct tcttcagatg gcaggccgag atagcgtgca gaagctccag
    15421 tggcaataat cagcgcgtcg caagtgtatt cgccgctatc gccagtcaga cggaacggtc
    15481 gattctgcaa atcaactttg ttgatgtgat caaagatgat ttcagtttca aatttggtgg
    15541 catgttcgtg catgcgctcc attaataacg gaccggtcag atcgtttgga tcgccaggcc
    15601 agttttccac ttccgtggtg gtggtcagtt ggccgccttt ttccatgccg gtaatcagca
    15661 caggttgcag gttggcgcgc gctgcataga cagcagcggt gtatcccgcc gggcctgaac
    15721 ccaggataag cagtttactg tgtttggtcg tgcccatgag atccccatag ttgttggcag
    15781 acaatgggca ggattgtagg gaatttatag acgtaaaaaa agagtatgac gattttgtta
    15841 acaatttgtg caatcggcag catcgataag caggtcaaat tctcccgtca ttatcacctc
    15901 tgctacttaa atttcccgct ttataagccg attaaatgat gaataaacgc ccctgttaat
    15961 gaatatctgg catgttgtac taaaaatcga tgttttgctt tgacaatccc ctggtgtttt
    16021 gcgaaaacat tcgaggaaga aaaaaaacag tattcttata tgcgcataac catgcatgta
    16081 aataccatgt ttaccgtgct agtgaaatct acgtatggcg tggacagacg ccattcgtga
    16141 tgtcgatagc tgccacaagg caacggtctt ctcaccgtag acccaggcat tgcgcgccgt
    16201 gaatcttcat gatttcggtc tatcgtgacg ggtagcgact ctgaacagtg atgtttcaga
    16261 gtcagacagg agtagggaag gaatacagag agacaataat aatggtagat agcaagaagc
    16321 gccctggcaa agatctcgac cgtatcgatc gtaacattct taatgagttg caaaaggatg
    16381 ggcgtatttc taacgtcgag ctttctaaac gtgtgggact ttccccaacg ccgtgccttg
    16441 agcgtgtgcg tcggctggaa agacaagggt ttattcaggg ctatacggcg ctgctgaacc
    16501 cccattatct ggatgcatca cttctggtat tcgttgagat tactctgaat cgtggcgcac
    16561 cggatgtgtt tgaacaattc aataccgctg tacaaaaact tgaagaaatt caggagtgtc
    16621 atttagtatc cggtgatttc gactacctgt tgaaaacacg cgtgccggat atgtcagctt
    16681 accgtaagtt gctgggggaa accctgctgc gtctgcctgg cgtcaatgac acacggacat
    16741 acgttgttat ggaagaagtc aagcagagta atcgtctggt tattaagacg cgctaacacg
    16801 gaacaggtgc aaaatcggcg tattttgatt acactcctgt taatccatac agcaacagta
    16861 ctggggtaac ctggtactgt tgtccgtttt agcatcgggc aggaaaagcc tgtaacctgg
    16921 agagcctttc ttgagccagg aatacactga agacaaagaa gtcacattga caaagttaag
    16981 tagcggccgc cgccttctgg aggcgttgct gatccttatt gtcctgtttg ccgtctggtt
    17041 gatggctgcc ttactaagct ttaacccttc ggaccccagc tggtcgcaaa cggcctggca
    17101 tgaacctatc cataatttag gtgggatgcc cggtgcgtgg ctggcagata cgctgttctt
    17161 tatttttggc gtgatggctt acaccattcc cgtcattatt gtcggcggtt gttggtttgc
    17221 ctggcgtcat cagtccagcg acgaatacat tgattatttt gccgtttcgc tacgcatcat
    17281 tggcgttttg gcgctcatcc ttacctcctg tggtctggcg gcaatcaacg ctgacgatat
    17341 ctggtatttt gcctccggtg gcgtcattgg cagcttacta agtactacgc tacaaccact
    17401 gctacacagt agcgggggaa ctattgcgct gctctgcgtt tgggcagcgg gcctgacgct
    17461 gttcaccggt tggtcatggg tgaccattgc tgaaaaactc ggcggctgga ttttaaacat
    17521 tctcaccttc gccagtaatc gtacccgtcg cgatgatacc tgggtcgatg aagatgaata
    17581 tgaagatgac gaagagtatg aagatgaaaa ccacggcaaa cagcatgaat cacgccgtgc
    17641 tcgtattctt cgcggcgcgc tagcgcgtcg taaacggttg gcggaaaaat tcattaatcc
    17701 gatggggcgg caaacagacg ctgcgttgtt ctccggtaag cggatggatg atgacgaaga
    17761 gattacctac actgcacgcg gtgtggctgc cgaccctgac gacgtcctat tttcgggcaa
    17821 tcgtgcaacg cagccagaat atgacgaata cgatccatta ttaaacggtg cgccaattac
    17881 cgaacctgtc gctgtagcag ctgctgctac cacggcgaca caaagctggg ctgcgccggt
    17941 tgaacctgtg actcagacgc cgcctgttgc ctctgttgac gttccacctt cgcaacctac
    18001 agtagcctgg cagcctgtac cgggtccaca aacgggagag ccggttattg ctcctgcacc
    18061 ggaaggttac ccacagcagc cacaatatgc gcagcctgca gtgcaatata atgagccgct
    18121 gcaacaacca gtacagccac agcagccgta ttatgcacct gcagctgaac aacctgcgca
    18181 acagccgtat tatgcccctg cgccagaaca accggtggca ggtaacgcct ggcaagccga
    18241 agagcagcaa tccacttttg ctccacagtc tacataccag actgagcaaa cttatcagca
    18301 gccagccgct caggagccgt tgtaccaaca gccgcaaccc gttgaacagc agcctgttgt
    18361 ggagcctgaa cccgttgtag aagagacaaa acccgcgcgt ccgccgcttt actattttga
    18421 agaagtggaa gagaagcgag cccgtgaacg tgaacaactt gcggcctggt atcaaccgat
    18481 tccagaaccg gttaaagaac cagaaccgat caaatcttcg ttgaaagcgc cttctgttgc
    18541 agcagtacct ccagtagaag ccgctgccgc tgtttctccg ctggcatctg gcgtgaaaaa
    18601 agcgacactg gcgacggggg ctgccgcaac cgttgccgcg ccagtcttca gtctggcaaa
    18661 tagcggtgga ccgcgtccgc aagtcaaaga ggggattggc ccgcagttgc cacgaccgaa
    18721 acgtatccgc gtgccaactc gtcgtgaact ggcgtcttac ggtattaagc tgccctcaca
    18781 gcgtgcggcg gaagagaaag cccgtgaagc ccagcgcaat cagtatgatt ctggcgatca
    18841 gtataacgat gatgaaatcg atgcgatgca gcaggatgaa ctggcacgtc agttcgccca
    18901 gacacagcag caacgctatg gcgaacagta tcaacatgat gtgcccgtaa acgcagaaga
    18961 tgcagatgct gcggcagagg ctgaactggc tcgtcagttt gcgcaaactc aacaacaacg
    19021 ttattccggc gaacaaccgg ctggggcgaa tccgttctcg ctggatgatt ttgaattttc
    19081 gccaatgaaa gcgttgctgg atgatggtcc acacgaaccg ttgtttacgc caattgttga
    19141 acctgtacag cagccgcaac aaccggttgc accgcagcag cagtatcagc agccacaaca
    19201 gccggttgcg ccacagcagc aatatcagca gccgcaacag ccggttgcgc cgcagccgca
    19261 gtatcagcag ccacaacagc aggttgcgcc gcagccacaa tatcagcagc cgcaacaacc
    19321 ggttgcgcca cagccgcaat atcagcagcc gcaacaacca gttgcgccgc agcagcagta
    19381 tcagcagccg caacaaccgg ttgcaccgca gccgcagtat caacagccgc aacaaccggt
    19441 tgcaccgcag cagcaggata ccctgcttca tccgctgttg atgcgtaatg gcgacagccg
    19501 tccgttgcat aaaccgacga cgccgctgcc ttctctggat ttgctgacac cgccgccgag
    19561 cgaagtggag ccggtagata cctttgcgct tgaacaaatg gctcgcctgg tggaagcgcg
    19621 tctggctgat ttccgtatta aagccgatgt cgtcaattac tctccggggc cggttatcac
    19681 tcgctttgaa cttaacctgg ctcctggcgt aaaagcggcg cgcatttcta acttgtcacg
    19741 ggaccttgcc cgttcacttt cgacggtggc ggtgcgtgtc gttgaagtta ttcctggcaa
    19801 accctatgta ggtctggagt taccaaataa aaaacgacaa accgtttatc tgcgcgaagt
    19861 tttggataac gccaaattcc gcgataatcc gtcgccatta accgtggtgc tgggtaaaga
    19921 tatcgccggt gagccggtgg ttgccgatct ggcgaaaatg ccgcacttgt tggttgcggg
    19981 gactaccggt tccggtaaat ctgtcggtgt gaacgcgatg atcctgagca tgctttataa
    20041 agcacagcca gaagatgtgc gtttcatcat gatcgacccg aaaatgctgg agctttcggt
    20101 ttatgaaggc attccgcatc tgttaacgga agtcgttact gatatgaaag atgccgccaa
    20161 cgcgctgcgc tggtgtgtta acgagatgga gcgtcgttat aaactgatgt ctgcgctggg
    20221 tgtgcgtaat ctggcgggtt ataacgaaaa aattgctgaa gccgatcgca tgatgcgtcc
    20281 gattccagac ccgtactgga agccgggtga cagtatggat gcccagcatc cggtgctgaa
    20341 aaaagaacca tacattgtgg tgttggttga cgaatttgcc gacctgatga tgacggtagg
    20401 taaaaaagtg gaagagctga tagcacgtct ggcgcaaaaa gcccgtgccg cgggtatcca
    20461 cctcgtactg gcaactcagc gtccatcggt tgatgttatt actggtctga ttaaagcgaa
    20521 tattccgacc cgtatcgcct ttaccgtatc cagtaagatt gactcacgta ccattcttga
    20581 tcaggctggc gcggaatcac tgctggggat gggggatatg ctctactctg ggccgaactc
    20641 cacgttgccg gtacgtgtcc atggtgcttt tgttcgcgat caggaagttc atgccgtggt
    20701 gcaggactgg aaagcgcgtg gtcgcccaca gtatgttgat ggcatcactt ccgacactga
    20761 aagcgaaggt ggcgcgggcg gtttcgatgg cgctgaagaa ctggatccgt tgttcgatca
    20821 ggcggtgcag tttgtcactg aaaaacgcaa agcgtcaatt tctggcgtac agcgtcagtt
    20881 ccgcattggt tataaccgtg cagcgcgtat tatcgaacag atggaagcgc aggggattgt
    20941 cagcgaacag gggcacaacg gtaatcgtga agtgctggcc ccaccgccgt ttgactaatt
    21001 aatgcattgc cggataaggc gcggtagcgt cgcatccggc actctatcaa ctgaaaattc
    21061 agtattttct tctttcctca agctgattat tagcctggaa tagagagtag agggaactcc
    21121 cgatcgggag tgacgtaatt tgaggaataa tgatgaaaaa aattgccatc acctgtgcat
    21181 tactctcaag cttagtagca agcagcgttt gggctgatgc cgcaagcgat ctgaaaagcc
    21241 gcctggataa agtcagcagc ttccacgcca gcttcacaca aaaagtgact gacggtagcg
    21301 gcgcggcggt gcaggaaggt cagggcgatc tgtgggtgaa acgtccaaac ttattcaact
    21361 ggcatatgac acaacctgat gaaagcattc tggtttctga cggtaaaaca ctgtggttct
    21421 ataacccgtt cgttgagcaa gctacggcaa cctggctgaa agatgccacc ggtaatacgc
    21481 cgtttatgct gattgcccgc aaccagtcca gcgactggca gcagtacaat atcaaacaga
    21541 atggcgatga ctttgtcctg acgccgaaag ccagcaatgg caatctgaag cagttcacca
    21601 ttaacgtggg acgtgatggc acaatccatc agtttagcgc ggtggagcag gacgatcagc
    21661 gcagcagtta tcaactgaaa tcccagcaaa atggggctgt ggatgcagcg aaatttacct
    21721 tcaccccgcc gcaaggcgtc acggtagatg atcaacgtaa gtagaggcac ctgagtgagc
    21781 aatctgtcgc tcgatttttc ggataatact tttcaacctc tggccgcgcg tatgcggcca
    21841 gaaaatttag cacagtatat cggccagcaa catttgctgg ctgcggggaa gccgttgccg
    21901 cgcgctatcg aagccgggca tttacattct atgatcctct gggggccacc gggtaccggc
    21961 aaaacaaccc tcgctgaagt gattgcccgc tatgcgaacg ctgatgtgga acgtatttct
    22021 gccgtcacct ctggcgtgaa agagattcgc gaggcgatcg agcgcgcccg gcaaaaccgc
    22081 aatgcaggtc gccgcactat tctttttgtt gacgaagtgc accgattcaa caaaagccag
    22141 caggatgcat ttctgccaca tattgaagac ggcaccatca cttttattgg cgcaaccact
    22201 gaaaacccgt cgtttgagct taattcggca ctgctttccc gtgcccgtgt ctatctgttg
    22261 aaatccctgg gtacagagga tattgagcaa gtactaactc aggcgatgga agacaagacc
    22321 cgtggttatg gtggtcagga tattgttctg ccagatgaaa cacgacgcgc cattgctgaa
    22381 ctggtgaatg gcgacgcgcg ccgggcgtta aatacgctgg aaatgatggc ggatatggcc
    22441 gaagtcaatg atagcggtaa gcgggtcctg aagcctgaat tattgaccga aatcgccggt
    22501 gagcgaagcg cccgctttga taacaaaggc gatcgctttt acgatctcat ttccgcactg
    22561 cataagtcgg tacgtggtag cgcacccgat gcggcgctct actggtatgc gcgaattatt
    22621 accgctggtg gcgatccgtt atatgtcgcg cgtcgttgcc tggcaattgc ttccgaagat
    22681 gtcggcaatg ccgacccacg ggcgatgcag gtggcaattg cggcctggga ttgctttact
    22741 cgcgttggcc cggcggaagg tgaacgcgcc attgctcagg cgattgttta cctggcctgc
    22801 gcgccaaaaa gcaacgctgt ctacactgcg tttaaagctg cgctggcgga tgctcgcgaa
    22861 cgtccggatt atgacgtgcc ggttcatttg cgtaatgcgc cgacgaaatt aatgaaggaa
    22921 atgggctacg ggcaggaata tcgttacgct catgatgaag caaacgctta tgctgccggt
    22981 gaggtttact tcccgccgga aatagcacaa acacgctatt atttcccgac aaacaggggc
    23041 cttgaaggca agattggcga aaagctcgcc tggctggctg aacaggatca aaatagcccc
    23101 ataaaacgct accgttaatg ttatcgttgc ggtaatgttg ttactgtatc cctgtggtcg
    23161 caggctgtgg ccacatctcc catttaattc gataagcaca ggataagcat gctcgatccc
    23221 aatctgctgc gtaatgagcc agacgcagtc gctgaaaaac tggcacgccg gggctttaag
    23281 ctggatgtag ataagctggg cgctcttgaa gagcgtcgta aagtattgca ggtcaaaacg
    23341 gaaaacctgc aagcggagcg taactcccga tcgaaatcca ttggccaggc gaaagcgcgc
    23401 ggggaagata tcgagccttt acgtctggaa gtgaacaaac tgggcgaaga gctggatgca
    23461 gcaaaagccg agctggatgc tttacaggct gaaattcgcg atatcgcgct gaccatccct
    23521 aacctgcctg cagatgaagt gccggtaggt aaagacgaaa atgacaacgt cgaagtcagc
    23581 cgctggggca ccccgcgtga gtttgacttt gaagttcgtg accatgtgac gctgggtgaa
    23641 atgcactctg gcctcgactt tgcagctgca gttaagctga ctggttcccg ctttgtggta
    23701 atgaaagggc agattgctcg catgcaccgc gcactgtcgc agtttatgct ggatctgcat
    23761 accgaacagc atggctacag tgagaactat gttccgtacc tggttaacca ggacacgctg
    23821 tacggtacgg gtcagctgcc gaaatttgct ggcgatctgt ttcatactcg tccgctggaa
    23881 gaagaagcag acaccagtaa ctatgcgctg atcccaacgg cagaagttcc gctgactaac
    23941 ctggtgcgcg gtgaaatcat cgatgaagat gatctgccaa ttaagatgac cgcccacacc
    24001 ccatgcttcc gttctgaagc cggttcatat ggtcgtgaca cccgtggtct gatccgtatg
    24061 caccagttcg acaaagttga aatggtgcag atcgtgcgcc cggaagactc aatggcggcg
    24121 ctggaagaga tgactggtca tgcagaaaaa gtcctgcagt tgttgggcct gccgtaccgt
    24181 aaaatcatcc tttgcactgg cgacatgggc tttggcgctt gcaaaactta cgacctggaa
    24241 gtatggatcc cggcacagaa cacctaccgt gagatctctt cctgctccaa cgtttgggat
    24301 ttccaggcgc gtcgtatgca ggcacgctgc cgcagcaagt cggacaagaa aacccgtctg
    24361 gttcataccc tgaacggttc tggactggct gttggtcgta cgctggttgc agtaatggaa
    24421 aactatcagc aggctgatgg tcgtattgaa gtaccagaag ttctgcgtcc gtatatgaac
    24481 ggactggaat atattggcta atacccaatt tttctgaatc taaaaagcgc ctgcgggcgg
    24541 tttttgcatg agattaatta actgaaaata aaag
//
LOCUS       DAZFQD010000071        24436 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.071, whole genome shotgun sequence.
ACCESSION   DAZFQD010000071 DAZFQD010000000
VERSION     DAZFQD010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24436
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.071"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            153..1358
                     /gene="gltS"
                     /locus_tag="ACHKTW_002863"
     CDS             153..1358
                     /gene="gltS"
                     /locus_tag="ACHKTW_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015501 - glutamate:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015813 - L-glutamate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HFO6463853.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSMMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            1361..2227
                     /locus_tag="ACHKTW_002864"
     CDS             1361..2227
                     /locus_tag="ACHKTW_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6463854.1"
                     /translation="MKKRRKEPETLREHCRHIFGDEPPVLCVWETEFDYADAELKALA
                     AKEWQQISVWDLSAYYVLNLVYNEPMQIELFRYLFPLCLAQWHETVLAGGYGDHFEES
                     LMKALCRPYLWQEMMNASQRQQVRQFLLDTALQRMDNERGFNNVLCWLAVFNTLGGAA
                     PLIHSLWSRWWALDTPGKAVCAIQYAAHLIYPIEANPLWSQEWIGWGHPLGHKDGWSS
                     DNRAFLRQMLTPEMIVAGVQAAAEILRGEPEGAMAARIAQDAYEAMDILTIQIEDLLR
                     DLSCDESGHALE"
     gene            complement(2212..4293)
                     /gene="recG"
                     /locus_tag="ACHKTW_002865"
     CDS             complement(2212..4293)
                     /gene="recG"
                     /locus_tag="ACHKTW_002865"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFO6463855.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDTLKNKLLAALPFKPTGAQARV
                     VAEIERDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFAPLGIEVGWLAGKQKGKARLAQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACITEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPVEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(4299..4988)
                     /gene="trmH"
                     /locus_tag="ACHKTW_002866"
     CDS             complement(4299..4988)
                     /gene="trmH"
                     /locus_tag="ACHKTW_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0141100 - tRNA
                     (guanine(18)-2'-O)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HFO6463856.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            complement(4995..7103)
                     /gene="spoT"
                     /locus_tag="ACHKTW_002867"
     CDS             complement(4995..7103)
                     /gene="spoT"
                     /locus_tag="ACHKTW_002867"
                     /EC_number="2.7.6.5"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0008893 - guanosine-3',5'-bis(diphosphate)
                     3'-diphosphatase activity [Evidence IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HFO6463857.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVPKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            complement(7122..7397)
                     /gene="rpoZ"
                     /locus_tag="ACHKTW_002868"
     CDS             complement(7122..7397)
                     /gene="rpoZ"
                     /locus_tag="ACHKTW_002868"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFO6463858.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
     gene            complement(7452..8075)
                     /gene="gmk"
                     /locus_tag="ACHKTW_002869"
     CDS             complement(7452..8075)
                     /gene="gmk"
                     /locus_tag="ACHKTW_002869"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFO6463859.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            8333..10015
                     /gene="ligB"
                     /locus_tag="ACHKTW_002870"
     CDS             8333..10015
                     /gene="ligB"
                     /locus_tag="ACHKTW_002870"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HFO6463860.1"
                     /translation="MKVWMAILISILCWQSSVWAVCPAWSPARAQEDISRLQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFGSEPRDVMMPPLNGAVMHPVAHTGVRK
                     MVDKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSL
                     ISAVPQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQDDSDTLNSL
                     GVFVWAWPDGPQLMTDRLKELATAGFTLTQRYTRAVKNADEVARVRNEWWKAKLPFVT
                     DGVVVRGAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLA
                     PVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNSLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFS
                     WLLLTPEQLQNTPGIAKSKSAQLWHQFNLARKQPFTRWVMAMGIPLTRAALNARDERS
                     WSQLLFSTEQFWQQLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            complement(10012..10629)
                     /locus_tag="ACHKTW_002871"
     CDS             complement(10012..10629)
                     /locus_tag="ACHKTW_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HFO6463861.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(10921..11745)
                     /gene="dinD"
                     /locus_tag="ACHKTW_002872"
     CDS             complement(10921..11745)
                     /gene="dinD"
                     /locus_tag="ACHKTW_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HFO6463862.1"
                     /translation="MNEHHQPFEEIRHYGTEGQEFWSARELAPLLDYRDWRNFQKVLA
                     RATQACEASNQAASDHFVETTKMVVLGSGAQRELEDVHLSRYACYLVVQNGDPAKPVI
                     AAGQTYFAIQTRRQELADDEAFRQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATAI
                     DFAIFQNHGYRGLYGGLDQKAIHQRKGLKKSQKILDHMGSTELAANLFRATQTEEKLK
                     RDGVNSKQQANTTHFDVGSKVRQTIQELGGTMPEELPTPQVSIKQLENSVKITEKK"
     gene            complement(11966..12829)
                     /locus_tag="ACHKTW_002873"
     CDS             complement(11966..12829)
                     /locus_tag="ACHKTW_002873"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFO6463863.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMQSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            12956..13672
                     /gene="rph"
                     /locus_tag="ACHKTW_002874"
     CDS             12956..13672
                     /gene="rph"
                     /locus_tag="ACHKTW_002874"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFO6463864.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAICDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            13738..14379
                     /gene="pyrE"
                     /locus_tag="ACHKTW_002875"
     CDS             13738..14379
                     /gene="pyrE"
                     /locus_tag="ACHKTW_002875"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFO6463865.1"
                     /translation="MKPYQRQFIEFALSKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGIEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(14416..15012)
                     /gene="slmA"
                     /locus_tag="ACHKTW_002876"
     CDS             complement(14416..15012)
                     /gene="slmA"
                     /locus_tag="ACHKTW_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0010974 - negative regulation of division
                     septum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HFO6463866.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(15119..15577)
                     /gene="dut"
                     /locus_tag="ACHKTW_002877"
     CDS             complement(15119..15577)
                     /gene="dut"
                     /locus_tag="ACHKTW_002877"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFO6463867.1"
                     /translation="MMKKIDVKILDPRVGKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            complement(15555..16775)
                     /gene="coaBC"
                     /locus_tag="ACHKTW_002878"
     CDS             complement(15555..16775)
                     /gene="coaBC"
                     /locus_tag="ACHKTW_002878"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015941 - pantothenate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HFO6463868.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            16947..17615
                     /gene="radC"
                     /locus_tag="ACHKTW_002879"
     CDS             16947..17615
                     /gene="radC"
                     /locus_tag="ACHKTW_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFO6463869.1"
                     /translation="MKNNSQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHWVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            17832..18068
                     /gene="rpmB"
                     /locus_tag="ACHKTW_002880"
     CDS             17832..18068
                     /gene="rpmB"
                     /locus_tag="ACHKTW_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFO6463870.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            18089..18256
                     /gene="rpmG"
                     /locus_tag="ACHKTW_002881"
     CDS             18089..18256
                     /gene="rpmG"
                     /locus_tag="ACHKTW_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFO6463871.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            18354..19163
                     /gene="mutM"
                     /locus_tag="ACHKTW_002882"
     CDS             18354..19163
                     /gene="mutM"
                     /locus_tag="ACHKTW_002882"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HFO6463872.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(19202..19681)
                     /gene="coaD"
                     /locus_tag="ACHKTW_002883"
     CDS             complement(19202..19681)
                     /gene="coaD"
                     /locus_tag="ACHKTW_002883"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFO6463873.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPIKKP
                     MFTLEERVALAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(19689..20966)
                     /gene="waaA"
                     /locus_tag="ACHKTW_002884"
     CDS             complement(19689..20966)
                     /gene="waaA"
                     /locus_tag="ACHKTW_002884"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HFO6463874.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDADYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            21379..22437
                     /gene="rfaQ"
                     /locus_tag="ACHKTW_002885"
     CDS             21379..22437
                     /gene="rfaQ"
                     /locus_tag="ACHKTW_002885"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HFO6463875.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGLDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
     gene            22434..23558
                     /locus_tag="ACHKTW_002886"
     CDS             22434..23558
                     /locus_tag="ACHKTW_002886"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFO6463876.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFELIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYRLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGVDRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            23551..24348
                     /gene="rfaP"
                     /locus_tag="ACHKTW_002887"
     CDS             23551..24348
                     /gene="rfaP"
                     /locus_tag="ACHKTW_002887"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HFO6463877.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGM
                     NPLTRTSFIITEDLTPTISLEDYSADWATNPPDVRVKRMLIKRVATMVRDMHAAGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPRRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFAAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
BASE COUNT         5725 a         6045 c         6583 g         6083 t
ORIGIN      
        1 tttctgctta aaatccatgc catttgttca ttaacagctg agaatttgac ccaggaaagc
       61 ataactcgat acccgtcata gttcatgaag ttgccactgc gcgatgaagt atgacgagta
      121 tgaaagagtg atgcggatac aaaggagtaa ctatgtttca tctcgatact ttagcaacgc
      181 ttgttgccgc aacgctgacg ttgctgctcg ggcgtaagtt ggtccattcc gtctcctttt
      241 tgaagaaata caccataccg gaacctgttg cgggtggttt gttggtggcg ctggcgctac
      301 tagtactgaa aaaaagcatg ggctgggaag tcaactttga tatgtccctg cgcgatccgt
      361 taatgctggc tttcttcgcc accattggcc tgaacgccaa cattgccagt ttgcgtgccg
      421 gtgggcgtgt ggttggcatc ttcttgattg tggttgttgg tctgttggtg atgcaaaatg
      481 ccattggcat tggtatggct agcctgttag ggcttgatcc gctgatgggg ctgttggccg
      541 gttctattac gctttccggc ggtcacggta cgggcgctgc gtggagtaaa ttgttcattg
      601 aacgttatgg cttcaccaat gcgacggaag tggcgatggc ctgtgcaacg ttcggtctgg
      661 tgctgggcgg cttgattggc ggtccggtgg cgcgctatct ggtgaaacac tccaccacgc
      721 cgaacggtat tccggatgac caggaagtcc cgacggcgtt tgaaaagccg gatgtgggac
      781 gcatgatcac ctcgttggtg ctgattgaaa ctatcgcgct gattgctatc tgcctgacgg
      841 tggggaaaat tgttgcgcaa cttttggctg gcactgcttt tgaactgccg accttcgtct
      901 gtgtcctgtt tgttggcgtg attctgagca acggtctgtc aatgatgggc ttttaccgcg
      961 tctttgagcg tgcggtatcc gtgctgggta acgtaagcct gtcgttgttc ctggcgatgg
     1021 cgctgatggg gctgaaactg tgggagctgg cttcgctggc gctgccgatg ctggcgattc
     1081 tggtggtaca gaccatcttc atggcgttgt atgccatctt cgttacctgg cgcatgatgg
     1141 gcaaaaacta cgatgcggca gtgctggctg cgggtcactg tggttttggc ctcggtgcaa
     1201 cgccaacggc aatcgccaac atgcaggcga tcactgaacg ctttggcccg tcgcacatgg
     1261 cgtttttggt ggtgccgatg gtcggtgcgt tctttatcga tatcgtcaat gcgctggtga
     1321 ttaagctgta tttaatgttg ccgatttttg ccggttaaaa atgaaaaagc gtcggaagga
     1381 accggaaacg ctacgtgaac actgtcgcca tatttttggt gatgagccgc cagtgctctg
     1441 tgtatgggaa acggaatttg attacgcaga tgccgaactc aaggcgctgg cggctaaaga
     1501 gtggcagcag atcagcgtat gggatttgag tgcttactat gtactcaatc tcgtttataa
     1561 cgaaccgatg caaatagagt tgtttcgcta tctgtttccg ctctgcctgg cgcaatggca
     1621 tgaaaccgtt ttagctggcg gttatggtga tcacttcgaa gaatccttaa tgaaggcatt
     1681 gtgtcggccc tatctctggc aggagatgat gaatgcttcc caacgccagc aggtacggca
     1741 atttttactg gacaccgcgt tgcagcgtat ggataacgag cgtggtttca ataacgttct
     1801 ttgctggctg gcggtgttca atactctggg tggggccgcg ccgttaattc attctctctg
     1861 gtcgcgctgg tgggcgctgg atacgccagg aaaagcggtg tgtgctattc agtatgccgc
     1921 gcatctaatt tatcccatag aagccaatcc gttatggtcg caagaatgga taggctgggg
     1981 gcatccatta ggccataaag atggctggtc ctctgataat cgcgcttttc ttcgccaaat
     2041 gttaacacct gaaatgattg tggctggcgt acaggcagcg gctgaaattc tgcgtggtga
     2101 accggaaggc gcaatggcgg cgcgaattgc acaggatgcg tatgaggcaa tggatatcct
     2161 cactatccag attgaagatt tactgcgtga cttgtcttgt gatgagtctg gtcatgcgtt
     2221 ggagtaacgt tccgtctccg gcatccagcg ttctatcagg gcttttgctt gttgtgggta
     2281 acgttcgtga atatgtcgcg ccaggcgctg aacttccggg atcatcgcct gatcgcgcag
     2341 taaatccgcc actttaaact cagcattacc cgtctgacgc gtacctaaca gttcacccgg
     2401 tccgcgaatc tctaagtctt tctgtgcaat cacaaaaccg tcgttgctgt cgcgcagcac
     2461 ttgcagccga atttgcgcgg ttttggagag cggcgttttg tagagcagca cgcagtgaga
     2521 agccaccgca ccacgaccta cacgcccgcg aagctggtgt aactgcgcca gacccagacg
     2581 ctccgggttt tcgataatca tcagactggc gttaggcaca tcaacgccga cttcaataac
     2641 ggttgtggca accagcaggt gtagctcacc ttgtttaaac gacgccatca ccgcctgttt
     2701 ctcgacaggt ttcatccgcc cgtgtaccag gccaacgttc aactctggta gcgccagttt
     2761 caactcttcc caggtagctt ccgccgcctg cgcttccagc aattccgact cttcaatcaa
     2821 cgtacaaacc cagtatgcct gacgaccttc agttatgcag gcgtggcgca cgcggtcaat
     2881 gatgtcggta cggcgggtat caggaatagc gaccgtagtc actggcgtgc ggcctggcgg
     2941 cagctcatct atcaccgagg tatcgagatc ggcatacgca gtcattgcca gcgtgcgggg
     3001 gatcggcgtg gcggtcatga tcaactgatg cggatggaag ccctgctgct ggcctttctc
     3061 ccacaatgcc agacgctgat gcacgccaaa acgatgctgt tcgtcgataa tcaccagcgc
     3121 caggccgtta aactgcacct gttcctggaa gatggcgtgt gtaccgacaa tcatctgcac
     3181 ctgaccgctg gcgatggctt cctgctgtgc cagccgtgct ttacctttct gcttaccggc
     3241 gagccagccc acttcgatac cgagcggtgc aaaccagttg cggaagttat tggcgtgctg
     3301 ctcggcgagt aattcggttg gtgccatcaa tgctacctgt ttgccgtggg caatcgcacg
     3361 caacgcggcg agggcggcga ccagcgtttt accggaacct acatcgccct gcaccagacg
     3421 catcatcggc acatccagcg ccatatcgcg ctcgatctcc gccactacgc gtgcctgtgc
     3481 gcccgttggc ttgaacggta aggcggcgag gagtttattt ttcagcgtgt cattggcgct
     3541 cagcggctgg gcatgaaaac gctgtgctcc ggcacgtaag gctaacatgc tgaggttgtg
     3601 cgccagcagt tcttccagaa tcagacgacg ttgcgccgga tgctgcccgg tttccagatc
     3661 gctaagctgt agcgtcggtg gcgggcggtg caaagtgcgc aacgcttccg gtagcgtcat
     3721 cattccttgt gacagttccg gcggcaggag ttcttcaatg gcgcaggtgt cgagcagatc
     3781 cagcgcctgg tcggttaatt tacgcagcgt ggcctgcttt acgccttccg ttgttggata
     3841 aaccggcgtg agcgtttcct gtaattctgg cgtgctgaga tcgccctgca cgcggtattc
     3901 cgggtggatc atctccgcac catatttacc gcgctttgct tcgccataag ccagtacacg
     3961 gcggcccgtc gccaggctat ttttcattgc cgcgctgaaa ttgaaaaagc gcatggtgag
     4021 gatgccggaa ccgtcgctga tctggcaggt catcatccgc cgaccgccga aggagatatt
     4081 gcagttcagc acttcgcctt ccaccgtggc ataaacgccc ggcagtagtt ctccgatggg
     4141 gtagagatgg gtgcgatctt cgtagcgcag gggaaggtgt aagagtagat cctgcacggt
     4201 atgcaggttg attttcgcca gtttgttact aagtgctgcg ccaacgcccg ttagggaact
     4261 gagtgggaca gcatctaaca ggcgaccttt catggcactt accctgcagc ctgcatagta
     4321 gcccaccagt cggcatcagc ttcgatctcg ccttgctgat tgacgtgggg ataaggcagg
     4381 cctttgcgtt ttgcgacttt cgccagcacc ggatagccgc cttcaaacaa caggcgttgt
     4441 tgctctgctt ccggcaacat gctgttttca cgcaggtaca tgcctgcatt ttgccgctga
     4501 cgctgggctt cgtaaagaat gagggctgag gcaacggaaa cattcagcga ctgcaccatg
     4561 ccgatcatcg gaatgatgat gtcctgatcc gccagggcca atgcttcctg cgtgatgccc
     4621 gttttctcct gtcccatcaa aatgcaggtc gggcgagtgt aatcaatttc gcggaaatcg
     4681 acagcgttat cagaaagatg ggttgccaga atctgcatgc cctggccttt gagatgagcg
     4741 acggcatcgc caatggtgcg gtgtgttttc acctgtaccc agctgttact acccgccgct
     4801 gccgaagcca tggtgcgcat gcggctacca ggccagacgg cgtgaacttc atgtacgcca
     4861 acggcatctg cggtacgaat aatcgcagaa acgttatgag gtttgtggac ctgctccatg
     4921 cagacggtca gatcaggctg ccgcctggcg agcatttcgc agatgcgtgc ataacgtgtt
     4981 gggttcataa aacattaatt tcggtttcgg gtgactttaa tcacgtctgg catcacgcgg
     5041 attttgcgca tgatattcgc cagatgcaca cggtcacgag cggtcagacg aataaaggcg
     5101 ctgtagacgc gaccatcttt ctcttccgta ttcaaacttt gaatattcga ggtcgtggtg
     5161 ttaattgccg ccgtcaggtt tgccagcgca ccctgatgat tgaacatctc caccttgatt
     5221 tcggtgatga actcctgcgc cgtctcttta tcccattcca cagccataaa cttctctggc
     5281 tctttctggt agccacggat gttacggcag gattcatggt ggatcaccag acctttaccg
     5341 gggctgacgt gggcgataat cgggtcgcca ggaatggggc ggcagcattt cgcaaaggtg
     5401 atcagcacgc catcagcgcc tttgatcggc agatgcccgt ggctttgagt cgccggtgga
     5461 atggaggcgt ccccatgttg tagatttttc gcgaccacca cgctcattgc gttaccaagg
     5521 ccgatttctg ccagcagatc gtcaagcgtt gccagcttca tgcgatccag ctcgcgctga
     5581 atattttcct gcgggatttc attcagctta cggctaccac ccaaagcatg gttgagcaga
     5641 cgacggccca ggcttacaga atcatcacgc ttgaggtttt tcagcaactg acgaattttg
     5701 gcgcgcgctt ttgagctaac gacaaagttc agccacgcgg cattcgggcg agcgcccggt
     5761 gcggtaatga tttcaacggt ttgaccgctg gtaagcggct gcgacagcgg gtaaggctgg
     5821 cggtcaacgc gtgcgcccac gcaggcatga ccgatatcgg tatgcactgc ataagcgaag
     5881 tcgacgggcg ttgcaccggc aggcagctcg acaattcgcc cttctggtgt gaaaacgtaa
     5941 atctcatccg ggaagagatc ggatttaacg ctctcgataa attcaaacga actaccggcg
     6001 ctctgttgca gctccagcag gctttgcatc cagcgctggg cgcggatttg tgcggtagta
     6061 ctggtttcgc cgtgctcttt ataagcccag tgcgcggcaa cacccatctc cgccatctga
     6121 tccatatctt cggtacggat ctggacctca accggcacgc cgtgcgggcc aatcatcgag
     6181 gtgtgcaacg actgatagcc gttcgcttta ggaatggcga tatagtcttt cacgcgaccc
     6241 ggacgcggct tgtacaggct atgcatttgc ccgagcacgc gataacaggt gtcagaatca
     6301 ttgacgatca cgcggaaagc gtagatgtcc atgattgagt gaaaacgctg ctctttgggc
     6361 accattttgc agtaaatcga ataaagatgc ttttcgcgac cactgacgcg gcacggtatt
     6421 cccgcttcct gcaaacgccc ttcgatttca gagaggattt tttggatcat ctctttacgg
     6481 ttgccgcgcg cggctttcac cacttcttta attacgcggt aacgattggg atacagcgcc
     6541 tcaaaaccca gctcttcgag ttcggtttta atgtggtgga tacctaaacg gtgcgccagc
     6601 gggctataaa tttcgagagt ttcacgggcg atgcggcgac gtttgtccgg gcgaagtgag
     6661 cccagcgtgc gcatgttgtg ggtacggtcg gcaagtttga tgagaatgac gcggatatcc
     6721 tgcaccatcg ccataatcat cttgcgaaag ttttcggcct gcgcctcttt cttatcgcgg
     6781 aacttgagct tatcaagttt cgacaccccc tctaccagct cggcgacgct tttaccaaaa
     6841 agctgttcca tgtcctggta ggtggcggga gtatcttcaa tcacgtcatg cagcagcgcc
     6901 gccatcagcg tttcatagtc gagtttcatc tcggccagaa tgcaggcaac cgctaccggg
     6961 tgcgtgatat agggttcacc gcttgaacgt gtttgcccct cgtgagcatc acgtgcaacg
     7021 agatacgcct gccgcagacg cttgatttgg tcttccggca ggtaggtttg aatcagttga
     7081 ttcaggcttt caaacagata caagggcgac ccgctttgtg attaacgacg accttcagca
     7141 atagcggtaa cggcttgtaa ttcagcggct tcctgctctt gctgttcctg gcgttcgcga
     7201 acgtcgagga tctggttgtt gatcagacct tcttcgattt cgcgcagcgc gattacagtg
     7261 gttttatcgt tttcttccgg taccagcgga tcctttccgc ctacctgcat ctgacgagcg
     7321 cgacgcgcgg cgaccagtac caggtcaaaa cggttaccaa ttttctctac agcgtcctga
     7381 acagttacgc gtgccatact taaaaagctc cacaggtgaa gaaatgactg ggcatgatac
     7441 tgaaatcagg ttcagtctgc caacaatttg ctgattaaag cgtcatgacg ctgcttttgg
     7501 cggctcatgc gcagacgttc ggcgcgaata atggtcttca aatcggtcaa cgcggtatcg
     7561 aagtcatcat tcacaatcag ataatcatat tcggcgtaat ggctcatttc tgcaacagct
     7621 tgcgccatac gctttgcaat gacctcttcg ctgtcctgac cgcgaccgcg tagacggcgg
     7681 tccagttcaa ttttggacgg cggtaaaata aagatactcc gcgcgtgcgg cattttctgg
     7741 cgaatttgct gcgcgccctg ccagtcgata tcgagaaaaa catcgacacc ggtcgccagt
     7801 acttgctcaa tggcctcacg cgaagtgcca tagtaattac caaaaacttc tgcgtgttcg
     7861 aggaacgcat ctctgctaat catttcttta aattcatcat gattaacaaa gaaataatgt
     7921 tcaccgtgga cttcaccagg acgcggttgg cgtgtggtgt gtgaaacaga aacctgggtg
     7981 tcatacaacg gttgggtttt taataaagcc tgaatcaggc tggatttacc cgcgccactg
     8041 ggggcagaaa caatataaag cgtgccttga gccatgagta tctttcgtat gtgattagcg
     8101 aaataaagcc tacatacgag cttattatac acggcgcggc catgtgacgt agcccttgct
     8161 cacacttttt gcgcaaagtt gttgcgtttt gcgtagctgt ttccgcattt gcctgtgatt
     8221 tactgcaata aagacgattc tcaggaagcg tcctcgcaga ctccgttttt gccactcggc
     8281 tttgccttca ccacgttgta tatctgccag cctgactaca gcggaggtga agatgaaagt
     8341 atggatggcg atattaataa gtatcttgtg ctggcaatca tctgtgtggg cggtctgtcc
     8401 ggcctggtcg ccagccagag cacaggaaga tatttcccgc ctgcaacagc aaataaaaca
     8461 gtgggacgat gactactgga aggaaggaaa aagtgaggtg gaagacggtg tttacgatca
     8521 gttaagcgcc cgtcttacgc agtggcaacg ctgctttggg agcgagcccc gcgacgtgat
     8581 gatgccgcct ttaaatggtg cggttatgca tccggttgcc catacgggtg tacgcaaaat
     8641 ggtggataaa aacgcattaa gtctgtggat gcgagaacgt agcgatcttt gggtgcagcc
     8701 aaaagttgat ggcgttgcgg taaccctggt ttatcgggac gggaaactga acaaagcaat
     8761 cagtcgcggt aatggcctga aaggcgagga ctggacgcag aaagttagct taatttccgc
     8821 tgtgccgcaa accgttagcg ggcctttagc caacagtacg cttcaggggg aaatatttct
     8881 ccagcgcgag gggcatatcc aacaacaaat ggggggaata aatgcccgcg caaaagttgc
     8941 tggcctgatg atgcgccagg acgatagcga cacgctgaat tctctgggcg tttttgtctg
     9001 ggcatggccg gatggaccgc agttaatgac cgatcgttta aaggagctgg ctaccgcagg
     9061 ttttactcta acgcagaggt atacccgtgc ggttaaaaat gctgatgagg ttgcgcgcgt
     9121 acgcaatgag tggtggaaag cgaaattacc cttcgtcacc gatggcgtgg ttgtgcgagg
     9181 ggcgaaagaa cccgaatccc gccattggct accaggccag gcagaatggc tggtggcctg
     9241 gaaatatcaa cctgtagctc aggttgccga agtgaaggcg attcagtttg cggtgggtaa
     9301 gagcggtaaa atatcggtgg ttgcgtcact cgcacctgtc atgctggatg ataaaaaagt
     9361 ccagcgggtg aatattggtt ccgtcaggcg ctggcaggag tgggatattg cgcctggtga
     9421 tcagatcctc gtcagccttg ccggtcaggg tattcctcgc attgatgatg tggtctggcg
     9481 cggtgcagaa cgtacaaaac cgacaccacc agaaaaccgc tttaactcgt tgacctgcta
     9541 ctttgcttct gatgtttgtc aggaacagtt catttcacgc ttagtctggc tggggtcaaa
     9601 acaggttctg gggctggatg gcattggtga ggccggctgg cgcgcgctgc atcagactca
     9661 tcgctttgag catatctttt cctggctttt attaacgcca gagcaattac agaacacgcc
     9721 ggggatcgcg aaaagtaaaa gtgcgcagct atggcatcag tttaatctgg ctcgtaagca
     9781 gccttttact cgctgggtga tggcaatggg aataccgcta acccgggcgg cgcttaatgc
     9841 ccgtgatgaa cggtcctggt cgcaactttt atttagcacg gagcagttct ggcagcagct
     9901 gccggggact ggatcggggc gcgccagaca ggttattgaa tggaaggaaa atgcgcaaat
     9961 caagaagctg ggcagttggc tggctgccca gcagatcaca ggttttgaac cttagtgtcc
    10021 ttcgtggctg tagtaaaaga ccggtaatcc cagcttaagg cgtagcgcca gtaagcgggc
    10081 aaaaaagccg aataccaggg tggagataat taccacatca tggttagaaa catagtgttg
    10141 cagcgcgatg tacagcacgg cggaggcaaa tgagacaccg gcatacaact ctttctggaa
    10201 taccagtggg atacgtttac agaacatatc gcgcaaaacg ccgccaaaca cgccggtggt
    10261 taccgccgcg acaacggcaa taattgggcc gtgccccata tccagtgcaa cctgtgcgcc
    10321 gatgatagaa aagaccacca gtccaagcgc atcgagcacc agaaacactt tgcgcaggta
    10381 aggcattaca ggagcaacga tagtggtgag tactgcggcg gtagcgacga taatcacata
    10441 ttccgggtgt ttgacccagc cgagcggata gtggcccagc agaatatcgc gcactgaccc
    10501 tccgccaatt gcggtggcgg tcgcaataat aattacgcca aatgtatcca tgcggcgtcg
    10561 cccggccgcc agagcccccg tcatggcttc ggcagtaatg ccaaccaaat acaaaatgtg
    10621 taacagcatg gttctctccc taaaaatctg tctggcaggg tagcgatttg tgcgtattgt
    10681 cacgattgag attttttaag gtgagaggaa attgataact tgtagttatg tgaaatcgct
    10741 attttctgta gcgtagatgc attcttctga cttcttgtct gagaaaatgc ataagaaaaa
    10801 ataggttaaa attgaggggt ggaaaagaaa ggtgcaatgt ttagccattg tgttcaccgc
    10861 acctggactt tggcttacgc atcgggtatc tcaatcaaca aaaagagttt ttagtttctg
    10921 ctatttcttc tctgtaattt ttacactgtt ttccaactgc ttgatgctga cctgcggggt
    10981 cggcaactcc tcaggcatgg ttccgccaag ttcctgaatg gtttgcctca ccttgctacc
    11041 cacgtcaaag tgtgtggtat tagcttgctg ttttgaattc acgccatccc gcttgagttt
    11101 ttcttctgtt tgggtagctc gaaagagatt agccgccagt tctgttgaac ccatatgatc
    11161 caggatcttc tgactctttt tcagcccctt ccgctgatgg atggctttct gatctaatcc
    11221 accatacagc ccccggtaac catgattctg aaatatggcg aaatcgatag ctgtcgctac
    11281 cccagcttgc tgcgctgcct caaccaattg tttgttatgt tctttcaatt catttctcag
    11341 aaaaagacgc ttttcgtctt cacgaagttg cctgaaagct tcatcgtcag ccagctcctg
    11401 ccgtcgggtc tggatagcaa aataagtttg ccctgccgca ataaccggtt tcgcagggtc
    11461 gccgttttgt actaccaggt aacaggcata gcgggagaga tgaacatctt caagttctcg
    11521 ttgagcccct gaacctaata caaccatttt ggtggtttca acgaaatggt cagatgcagc
    11581 ttgattgctg gcttcgcacg cttgagtcgc gcgggccagt accttttgaa aattgcgcca
    11641 gtcccgataa tccagtaacg gtgccaattc tctagctgac caaaattctt gcccttcagt
    11701 gccataatgc cgtatttctt caaaaggttg atgatgttcg ttcattgccc actccatgtg
    11761 atatcgattg caaagaaagt aaatctgtaa ctgtatttat atacagttgt gagtatctca
    11821 cgttctcagt gtgctgggca aaaaaacatc gcaaaatctg gagcaggcac gcaaaacaga
    11881 attgaagttg cggggaagaa aaatgccgct ctgtttgagt gggcggcatt tgtgaaggct
    11941 gaaaagtaag aattggcgtt acgagttatt caatgttctg aatctgctcg cgcatctgct
    12001 caatcaacac tttcagctca atcgcggagt ttgtcacttc ggcattgata gacttcgacg
    12061 caagagtgtt cgactcgcgg ttgaattcct gcatcataaa gtccagacga cgaccaacgg
    12121 cttctttttt cttcagaatg ttgtaggtct ctttgacatg cgcttccagg cggtccagtt
    12181 cttcggcaac gtcaattcgt tgtgccagca gaaccagctc ctgctccaga cggttgtttt
    12241 ccaactgaac ctgagcatct tccagcttcg cgaccagacg ctcacgctgc cattgcagga
    12301 tttccggcat atgggcgcgg actttgacca cttcggcggt gacgccttcc agacgctgct
    12361 cgatcaatgc tttcagtgcc tgaccttcgg tttcgcgcgc gacaataaag tcgtccagcg
    12421 taccatccag cgccgcgaga atttcagcgg caatggcgtc aagatcctgc tcctgggctg
    12481 ccatcacgcc cggccagcgt agaatatcaa ccgggttgat ttccccttcg tcactctgca
    12541 tttttaccca gttcgcggca gttaccagct gtttagccag tttttcgttg aggatcagct
    12601 caccttgcgc gctaacatct ggctcatagc gcagggtaca ttccacttta ccgcgcgtca
    12661 ggcgagaacg aatacgctcg cgaacgacag gttcaaggct acggaactgc tccggcagac
    12721 gaaagtaagt ttccagataa cgctggttta ccgagcgcat ttcccaggtt gcgctccccc
    12781 attcaccctt gatttcacgc cgggcgtagg cggtcatact gcggatcata gacgttcctg
    12841 tttataaaag gagaggtgga aggattatag ccatcgatgc cttgtcagga taggaataac
    12901 cgccggaagt ccgtataatg cgcagccaca tttgtttcaa gccggagatt tcaatatgcg
    12961 tccagcaggc cgtagcaata atcaggtgcg tcccgttacc ctgactcgta actatacaaa
    13021 acatgcagaa ggctcggtgc tggtcgaatt tggcgatacc aaagtgttgt gtaccgcctc
    13081 tattgaagaa ggcgtgccgc gcttcctgaa aggtcagggc cagggctgga tcaccgcaga
    13141 gtacggcatg ctgccacgtt ctacccacac ccgtaacgct cgtgaagcgg cgaaaggtaa
    13201 gcagggtgga cgcacaatgg aaatccagcg tctgatcgcc cgtgctcttc gcgcggcagt
    13261 agatttgaaa gcgctgggtg agttcaccat tacgctggac tgcgacgtgc ttcaggctga
    13321 tggtggcacg cgtaccgcgt cgattacggg tgcctgcgtg gcgctggcag atgcgctaca
    13381 gaagctggtg gaaaacggca agctgaaaac caacccgatg aaagggatgg tagccgcagt
    13441 ttctgtcggt attgtaaacg gtgaagcaat ttgcgatctg gaatacgttg aagactcagc
    13501 cgcagagacc gacatgaacg tagtgatgac cgaagacggg cgcatcattg aagtgcaggg
    13561 gacggcagaa ggcgagccgt tcacccatga agagctactc accttgttgg cgctggcccg
    13621 agggggaatc gaatccattg tagcgacgca gaaggcggcg ctggcaaact gatttttaag
    13681 gcgactgatg agtcgccttt tttttgtctg tagaaaagta agatgaggag cgaaggcatg
    13741 aaaccatatc agcgccagtt tattgaattt gcgcttagca agcaggtgtt aaagtttggc
    13801 gagtttacgc tgaaatccgg gcgcaaaagc ccctatttct tcaacgccgg gctgtttaat
    13861 accgggcgcg acctggcact gttaggccgt ttttacgctg aagcattggt ggattctggc
    13921 attgagttcg atctgctgtt tggccctgct tacaaaggga tcccgattgc gaccactacc
    13981 gccgtggcgc tggcggagca tcatgacctt gacctgccgt actgctttaa ccgcaaagag
    14041 gcaaaagacc acggtgaagg cggcaatctg gttggtagcg cgttacaagg acgcgtaatg
    14101 ctggtagatg atgtgatcac cgccggaacg gcgattcgcg aatcgatgga gattattcag
    14161 gctaatggcg cgacgcttgc tggcgtattg atttcgcttg atcgtcagga acgggggcgc
    14221 ggcgagattt cggcgattca ggaagttgag cgtgattaca actgcaaagt gatctctatc
    14281 atcaccctga aagatctgat tgcctatctg gaagagaagc cggaaatggc ggaacatctg
    14341 gcggcggtta aggcctatcg cgaagagttt ggcgtttaaa gaaactcgcc ggatgaaaag
    14401 tcatccggcg tcatattact gcaactgcgc ggcaattagc ggccagcggg cgtcaaaatc
    14461 atccgtcggg cggtatttaa attcgctgcg gacaaaacgt gacagcatac cttcacagaa
    14521 ggccaggatc tggcttgcca gcagggtttc atcggtggtg taaccttcac cctcacgcat
    14581 tctcttttca cgcaatacct ggcgcagctg cgcttcaata cgctcgaaca gctggttgat
    14641 acgcccttgc aggcgatcct gttcaaacat cagcgcatga ccagtgagga tgcgggtcag
    14701 gccaggatta cgctcaccaa aaccgagaag cagcaacaca atcagacgca ggcgcgctgt
    14761 ggtgtctttc tcatctttca gaatcaggtt gatgcgagta atcaggctat cttcgataaa
    14821 ctcaatcagg ctatcgaaca tgcgggtctt actggggaag tggcgataca gtgccgcttc
    14881 ggaaacgccg acagaggcgg ccagttttgc cgtcgtgata cgttggcttc catcgctgga
    14941 ttccagcatc agcgccagag actgaagtat ttcctcgcga cggttccttt tcgcagtttg
    15001 tttttctgcc atgttacaaa atacccctga aaataagcac ttgccaggcg gcacccacgc
    15061 tatgaccgca aacgaaatgt ttgcggctat gttatgacgt tattcggatg cgtatgtgtt
    15121 actgacgacc agagtgacca aagccgcctt caccgcggtc ggtggcgtcg aaatcttcca
    15181 ccagattaaa ttcagcctgt actaccggaa caaaaatcat ctgggcgatg cgttcgccag
    15241 gttgaatggt gaagctgtcc tgaccacggt tccacacgga aatcatcaac tggccctgat
    15301 agtcagaatc gatcaatcct accaggttac caagcacgat accgtgctta tgtcccaatc
    15361 cggagcgcgg cagcatcatt gccgccagtg aaggatcggc aatatgaatc gccagcccgg
    15421 tcggaaccag cgtagtgtca cccggagcca gttctacggc gtcgtcgaga caggcacgca
    15481 ggtcaagtcc ggcagagcca gaggtggcat aagtcgggag cggaaattcc ttcccaacgc
    15541 gcgggtccag aatcttaacg tcgatttttt tcatcataac gggtcacgat ctcgtcgagt
    15601 aataattggc caaggagctc tttgcgctca agcggtaaga ctttatctcc gtcctgccag
    15661 aaaaggtgta atgcgttgtt gtcgctgtta aatccttgag ttggctggga aacatcgttc
    15721 gcgcagatca gatcaaggtt tttacggata cgtttttgcc gggcgtattc ttccacatta
    15781 tttgtttcgg cggcaaatcc aacgacgtag ggtcgatggt cttttagtgc ggcaacgcct
    15841 gcgacgatat cggggttttt aaccattttt attgttaatt catcaccctg cgtggcctgc
    15901 tttttgattt tctctggggc cacggtagct gcgcgataat ccgccacggc ggcgcagccg
    15961 ataaaaatat tttgctgctg tacagaagca ttcacggcgg cttccatttc cagcgcggtc
    16021 atcacatcaa cacgtttaac aaacggtggc gtcggtagtg aaaccggacc tgataccagc
    16081 gtgacgttcg cgccacgacg ggcagcggcg gcggcgatag caaaacccat cttgccggag
    16141 ctgtgattag agatataacg cacgggatcg agcggttcac gcgtcgggcc ggcggtaatc
    16201 ataatgttca gatgtttcag gtcgttgacg ggcgaaaaat gcgctaccgc catatccaca
    16261 atggttaacg gatcgagcat tcgcccagga ccgatatcac cacaagcctg actgccactg
    16321 tctggccccc agatgagcaa accacgggaa gcaagcacct ctaaattatg ctgcgtggca
    16381 gcggcacggt acatctgctg gttcatggcg gggagcacgg ctacaggcgc aggtgtagcc
    16441 agacaaatcg tcgataccag gtcattcgcc attccggcag caacacgggc aatcaaatct
    16501 gccgtggcag gggcgagaat cactaaatca gcccatttac ccagctcaat atggcccata
    16561 gcggcttcgg ctgccgggtc cagcagactg tcggaaacgg gataaccaga aaccgcctgc
    16621 aagctaagtg gggtgataaa ggcttttgcc gcttcggtca tggctacgcg gacgtcggcc
    16681 ccgcgatcgc gcaaacgacg caccagttca ggggttttat aggcagcaat accgccgcta
    16741 acgccgagaa cgattttttt accggccagg ctcatgatga tttttcctgt tgggtgacac
    16801 cagaagttgg cgattttatc acaatacttt tgttgtcgtg ccttcacctg agaatcactt
    16861 tgcgaggcgc tttccaggat tgaaaactgg ccgtcgattt aacggaacgg ctatgacagg
    16921 atgcgagcac cacaaaggag gtgaaggtga aaaacaattc acagctgttg atgccgcgcg
    16981 aaaaaatgct gaagtttggt attagcgcct taacggatgt cgagctgctg gcgctatttc
    17041 tgcgtaccgg aacgcgcggt aaagatgtat taaccctggc aaaagagatg ctggagaatt
    17101 tcggctctct ttatggcttg ttaacctctg aatatgaaca atttagtggc gttcatggaa
    17161 ttggcgtggc gaaatttgcc cagttaaagg ggattgctga actggcgcgg cgttactaca
    17221 acgtgcggat gcgtgaagaa agccctttac tcagcccgga gatgacgagg gaatttttac
    17281 aaagccagct cacgggtgag gagcgggaga tctttatggt gatctttctc gactcccaac
    17341 actgggttat aacgcatagc cgtctttttt ccggcacgct aaaccatgtt gaagtccatc
    17401 ctcgggaaat tatccgcgaa gcgataaaaa taaacgcctc ggcgctgatc cttgcacata
    17461 atcacccttc gggttgtgct gaacccagta aagcggataa actcattact gaacggataa
    17521 taaagagttg tcagttcatg gatttacgcg tgctcgacca tatcgtgatt gggcgtggag
    17581 agtatgtttc ttttgccgaa cgcggctgga tttaacccgc tatgcgcgat ccttcgggat
    17641 ctttgtctgt tcgggacttg agcacatcgc tgagtcagcg tatactacgc cacctttgag
    17701 aatctcgggt ttggcatttg ggcctggcaa tcgagagttc acatagaact gcgatgaccg
    17761 ggctgtaaag cctgacgagg cgccaatacc ccatacgaag ctcgagctaa tttgattttt
    17821 ggagaataga catgtcccga gtctgccaag ttactggcaa gcgtccggtg accggtaaca
    17881 accgttccca cgcactgaac gcgactaaac gccgtttcct gccgaacctg cactctcacc
    17941 gtttctgggt tgagagcgag aagcgttttg tcaccctgcg cgtatctgct aaaggtatgc
    18001 gtgtaatcga taaaaaaggc atcgatacag ttctggctga actgcgtgcc cgtggcgaaa
    18061 agtactaagt acttagagga aataaatcat ggctaaaggt attcgtgaga aaatcaagct
    18121 ggtttcttct gctggtactg gtcacttcta taccactacg aagaacaaac gtactaagcc
    18181 ggaaaaactg gaactgaaaa aattcgatcc agttgttcgc cagcacgtga tctacaaaga
    18241 agcgaaaatc aaataattct cgctttgatg taacaaaaaa cctcgctccg gcggggtttt
    18301 ttgttatctg cttgccccca tattgactgc atctgttcat tcctggagat gctatgcctg
    18361 aattacccga agttgaaacc agccgccgcg gcatagaacc gcatctcgtt ggtgcaacca
    18421 ttcttcatgc ggtggtgcgc aacggacgct tgcgctggcc ggtttcagaa gagatctacc
    18481 gtttaagcga ccaaccagtg cttagcgtgc agcggcgggc taaatatctg ctgctggagc
    18541 tgcctgaggg ctggattatc attcatttgg ggatgtctgg cagcctgcgc atccttccag
    18601 aagaacttcc ccctgaaaag catgaccatg tggatttggt gatgagcaac ggcaaagtgc
    18661 tgcgctacac cgatccgcgc cgctttggtg cctggctgtg gaccaaagag ctggaagggc
    18721 acaacgtgct ggctcatctt ggaccggagc cgcttagcga cgatttcaat ggtgagtatc
    18781 tgcatcagaa gtgcgcgaag aaaaaaacgg cgattaaacc gtggctgatg gataacaagc
    18841 tggtggtagg ggtagggaat atctatgcca gcgaatcact gtttgcggcg gggatccatc
    18901 cggatcggct ggcgtcatca ctgtcgctgg cagagtgtga attgttagct cgggtgatta
    18961 aagcggtgtt gctgcgttcg attgagcagg gtggtacaac gctgaaagat tttctgcaaa
    19021 gtgatggtaa accgggctat ttcgcacagg aattgcaggt ttacgggcga aaaggcgagc
    19081 cgtgtcgggt gtgcggtacg ccgattgtgg cgactaaaca tgcgcagcgg gcaacgtttt
    19141 attgtcggca gtgccagaag taattcatgc gcgccggatg gcataccatc cggcataaac
    19201 gctacgctaa cttcgccatc agcgcctgat ggacattctc cggcaggaaa tgggtgacat
    19261 cgccctgatg gcgcgccacc tctttcacca acgatgaaga gataaacgac cactctttcg
    19321 acggcatcag aaacacactt tccagttccg gcattaagtg gcgattcata tgcgccagct
    19381 gcatttcata ttcaaaatcc gccaccgcac gcaggccacg aatcagcacc gtagcgtgtt
    19441 gattacgggc gaagttcgcc atcaaatcac taaacccgac cacttccacg ttccccagat
    19501 gcgcggttgc ctgctgtgcc agtgccacac gctcttccag ggtaaacatc ggttttttaa
    19561 tggggctggc ggcaatcgcc agaataacgt gatcgaacat ctgcgtggcg cgcgtcacga
    19621 tatcgatatg accattggta atgggatcga aagtacccgg ataaatcgcc cgtttttgca
    19681 taacaacctc aatgcgtttt cggtggcagg taaggttcca gcagttgaag cagacgctgt
    19741 agcgcgccct ggttttgata cagtacttca acggcatgac ggccatagaa actacggtaa
    19801 tcggcgtcgg tgagtaaaga ggacacctct tttgcaagcg tagtggcatc ggtaacggta
    19861 atcagcccgc ttgcctgctc cagccgcgcg caaatgtctt taaagttaaa agtatgcggc
    19921 cccatcaata ccggaatagc gtgtgcggca gcttccagcg gattatgccc accacgttca
    19981 accagtgaac cgccaacaaa ggcgagatcg gcaatgccgt acagtaacat caactcgccc
    20041 atcgtatcgc caactacaac ctgcgtgctg gtggagggga cttcccctga agagcgtgtg
    20101 atatagctta gtccagcctg gcggacaaga ttaatcgcat ccgggaagcg ttctggatga
    20161 cggggtacca ggatgagcaa taaattcggg aattgctgta acaatgcctg atgtgcggcg
    20221 atcaccacac tctcttcgcc ttcgtgagtg ctggtggcaa tccataccgg gcggtgtggt
    20281 gcccactggc ggcgcagcgt cacggcttta gcagccaact gcggcgttac agaaatatcg
    20341 aatttcaggc taccggtaac ggtcacctga ttattttttg cgcccagcgc cacaaaacgt
    20401 gcaccatctt cttcattttg cgcagcaatc agcgtaatac gacgcagcaa gcgacggacg
    20461 aatttaccca gtttggcata acctgcggcc gagcgggcag agagtcgcgc gttagcgatc
    20521 accagcggaa ttttacgttt gtgtagcgcc gcaatcaggt taggccatag ttcggtttcc
    20581 ataatcaaca ccagtttggg gtcgacttta ttcaggaaac ggttgagcgc atcgggcagg
    20641 tcatacggca gataaacgtg ctgaacatcc ttcccgaaag ccgattgtac gcgctccgaa
    20701 ccggttggcg tcatggttgt aacggtaatc ggtaaatcag gataacgatg gcgcagcgcg
    20761 cgcaccaacg ggatcgccgc cagagtttca ccgacggaga cggagtgcag cataatgccg
    20821 cctggtttta gcggatggcg gtaaaaaccg taacgttcac cccagcgttt tcgataggcc
    20881 ggagccttac gtccgcgcac ccagagccgt atccagatca gcggctgaat aaggtagaga
    20941 agggcggtgt aaagcaattc gagcatagta aatagctgac ttatggatgt gctggggatt
    21001 ctatgtattt agctgtggct ttaccattac ttttcccgtt tttgacttaa atagcaattt
    21061 acccgccaat tcttgctgaa catctatact ttttttgtat ttttatgcga ttcattgatt
    21121 tctgctgtac gtgcaggaag gaatcgcaga gcaacagtga aagaatatgg ttgctaatga
    21181 ttttatttga ttgatttctt tattgaaaat caacaatgtg cttaaaaatc attcgggtcg
    21241 gattgccgcg aaagaagtga tacactagcg cgtcaatatc agtgcggtat cagtattcag
    21301 gtagctgttg agcctggggc ggtagcgtgc tttttctgct tcaccttaat cggataatct
    21361 caacaaaaag agttacttgt ggataagcca tttcgaagaa ttttgctcat taagatgcgt
    21421 tttcatgggg atatgttatt aactactccc gtcattagtt cgctgaaaaa aaattaccct
    21481 gacgcaaaaa tcgatgtgct gctttatcag gacaccatcc cgatcctgtc tgaaaatcca
    21541 gagattaacg cgctctacgg cataaaaaat aaaaaagcaa aagcctcaga aaaaattgcc
    21601 aacttttttc atctcatcaa ggtattacgt gccaataagt atgaccttat cgtcaatctt
    21661 accgatcaat ggatggttgc tatactggtt cgcttattaa atgcccgtgt gaaaatttcc
    21721 caggattatc atcatcggca gtctgctttt tggcgtaaaa gtttcaccca tttggtgccg
    21781 ttgcagggtg gaaatgtggt ggaaagtaac ttatccgtgc tgacaccatt gggacttgat
    21841 tcgttggtga agcagacaac catgagttac ccgcctgcaa gctggaaacg tatgcgtcgc
    21901 gaacttgatc acgctggtgt tggacaaaat tatgtggtta tccaacctac ggcgcggcaa
    21961 atcttcaaat gctgggacaa cgccaagttt tccgctgtga ttgatgcctt acatgctcgt
    22021 ggttatgaag tcgttctgac gtccggccca gataaagacg atctggcctg cgtcaatgaa
    22081 attgcgcagg gatgccagac gccaccagta acggcgctgg ctggaaaggt gaccttcccg
    22141 gaacttggtg cgttaatcga tcatgcgcag ctgtttattg gcgttgattc cgcaccggcg
    22201 catattgccg ctgcagttaa tacgccgctg atatcgctgt ttggtgcgac agatcatatt
    22261 ttctggcgtc cctggtcaaa taacatgatt caattctggg cgggagatta ccgggaaatg
    22321 ccaacgcgcg atcagcgtga ccgaaatgag atgtatcttt cggttattcc ggcggcagat
    22381 gtcattgctg ctgtcgataa attactgccc tcctccacga caggtacgtc gttatgatcg
    22441 ttgctttttg tttatataaa tattttccct ttggcggttt gcagcgtgat tttatgcgta
    22501 ttgctcagac agtcgccgcc cgaggtcatc atgttcgggt ttatacccag tcgtgggaag
    22561 gcgaatgccc tgatgtattt gaactgatca aagtgccggt taaatcgcat accaatcacg
    22621 ggcgcaatgc ggagtatttt gcctgggtgc aaaaacattt acgcgaacat cccgtcgata
    22681 aagtcgttgg attcaacaaa atgccggggc tggacgttta ttatgccgct gatgtttgtt
    22741 atgccgagaa agtagcgcag gaaaaaggct ttttctatcg cctgacgtca cgttatcgcc
    22801 attatgccgc ctttgagcgg gcaaccttcg aacagggcaa gccgacacag ctgctgatgc
    22861 tgacagataa gcaaatcgcc gatttccaga aacattatca gactgaagcg gagcgttttc
    22921 atattctgcc accggggatt tatcctgatc gtaaatatag ccagcagcca gccaatagcc
    22981 gtgaaatctt ccgtaagaag aatggaataa ccgaacaaca atatttattg ttgcaggtcg
    23041 gttcagactt cacgcgtaaa ggtgtcgatc gttccattga agcacttgct tcgttaccgg
    23101 attcgctgcg ccacaacaca ttgctatatg ttgttgggca ggataaaccg cgaaaatttg
    23161 aggcgctggc agaaaaacgc ggcgtgcgca gtaatgttca ctttttctcg gggcgcaatg
    23221 atgtctcgga attaatggcg gcggcggatt tattactgca tcctgcctac caggaagcgg
    23281 cgggaattgt gctgctggaa gcgattactg caggattacc ggtactaaca acagccgttt
    23341 gtggctatgc gcattatatt gtcgacgcta attgcggcga ggctattgct gagccattcc
    23401 gccaggaaac attgaatgag attttacgca aagcgttaac gcaatcttca ttgcgccagg
    23461 cctgggcgga aaatgcgcga cattatgctg atacacaaga tttatacagt ctgccagaga
    23521 aagcggcgga tatcataacg ggtggtctgg atggttgaac ttaaagagcc gtttgccacg
    23581 ttatggcgcg gcaaagatcc ttttgaggaa gttaaaacct tgcagggtga ggtatttcgt
    23641 gaactggaaa ctcgccgtac tctgcgcttt gaaatggcgg gcaaaagcta ttttctcaaa
    23701 tggcatcgcg gcacgaccct gaaagagata atcaaaaatt tactctcatt gcggatgcca
    23761 gtattaggcg ctgaccgcga atggaatgcg attcatcgac tgcgggatgt cggcgttgat
    23821 actatgtatg gggtggcatt tggcgaaaaa ggcatgaatc cgctgaccag aacttcattt
    23881 attattaccg aagatctgac accaaccata agtcttgaag attacagtgc tgactgggcg
    23941 actaaccctc cagatgttcg cgtaaagcgt atgcttatta agcgtgtcgc gacgatggtg
    24001 cgcgatatgc atgctgcggg cattaaccac cgtgactgtt atatctgtca tttcctgctg
    24061 cacttgcctt tttccggtaa ggaagaggag ttaaaaattt cggtaattga cctgcaccgg
    24121 gcgcagcttc gcacgcgcgt tccacgtcgt tggcgggata aagatcttat tgggctttat
    24181 ttttcttcga tgaatatcgg cctgactcag cgggatatct ggcggtttat gaaagtgtat
    24241 tttgccgccc cgcttaaaga cattctcaag caggaacaag gactgctgtc gcaagcagaa
    24301 gcaaaagcca caaaaatcag ggaaagaacg attcgaaaat cgttgtaacc cgtaacttat
    24361 ttttgccaaa attttggata cagaataaat atgtctcaac tcaatgatag tgacatcatc
    24421 ctttttgagt ataatt
//
LOCUS       DAZFQD010000072        23970 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.072, whole genome shotgun sequence.
ACCESSION   DAZFQD010000072 DAZFQD010000000
VERSION     DAZFQD010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23970
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.072"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..407
                     /gene="ycgY"
                     /locus_tag="ACHKTW_002888"
     CDS             <1..407
                     /gene="ycgY"
                     /locus_tag="ACHKTW_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein YcgY"
                     /protein_id="HFO6463878.1"
                     /translation="SGVKNGADSEAVLAALIEHLELINPSGRLSQNTRSAMLQLREEE
                     WSEFFFWLLNSLECLDYVIINLTPESKKTLMSEHRNNIQVAIDALYRQRRYKSPGDDS
                     ETLTRRNDAIFGNHVWQTFAQYFPPGLEKPSV"
     gene            complement(486..2183)
                     /gene="treA"
                     /locus_tag="ACHKTW_002889"
     CDS             complement(486..2183)
                     /gene="treA"
                     /locus_tag="ACHKTW_002889"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HFO6463879.1"
                     /translation="MKSPAPSRPQKMALIPACIFLCFAALSVQAEETPVTPQPPDILL
                     GPLFNDVQNAKLFPDQKTFADAVPNSDPLMILADYRMQQNQSGFDLRHFVNVNFTLPK
                     EGEKYVPPEGQSLREHIDGLWPVLTRSTENTEKWDSLLPLPEPYVVPGGRFREVYYWD
                     SYFTMLGLAESGHWDKVADMVANFAHEIDTYGHIPNGNRSYYLSRSQPPFFAMMVELL
                     AQHEGDAALKQYLPQMQKEYAYWMDGVENLQAGQQEKRVVKLQDGTLLNRYWDDRDTP
                     RPESWVEDIATAKSNPNRPATEIYRDLRSAAASGWDFSSRWMDNPQQLNTLRTTSIVP
                     VDLNSLMFKMEKILARASKAAGDNAMANQYETLANARQKGIEKYLWNDQQGWYADYDL
                     KSHKVRNQLTAAALFPLYVNAAAKDRANKMATATKTHLLQPGGLNTTSVKSGQQWDAP
                     NGWAPLQWVATEGLQNYGQKEVAMDISWHFLTNVQHTYDREKKLVEKYDVSTTGTGGG
                     GGEYPLQDGFGWTNGVTLKMLDLICPKEQPCDNVPATRPTVKSATTQPSTKEAQPTP"
     gene            complement(2503..3921)
                     /gene="dhaM"
                     /locus_tag="ACHKTW_002890"
     CDS             complement(2503..3921)
                     /gene="dhaM"
                     /locus_tag="ACHKTW_002890"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019563 - glycerol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HFO6463880.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDSCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADADHVLVMMDMGSALLSAETALELLAPKIAAKVRLCAAPLVE
                     GTLAATVSAASGADIDKVIFDAMHALEAKREQLGLPSSDTKISDTCPAYDEEARSLAV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRQLAEDNFGETEEVAPPTLRPVPPVSGKAFYYQPVLCTVQAKSTL
                     TVEEEQDRLRQAIDFTLLDLMTLTAKAEASGLDDIAAIFSGHHTLLDDPELLAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEELPQ
                     FNSPTILLAENIYPSTVLQLDPAVVKGICLSAGSPVSHSALIARELGIGWICQQGEKL
                     YAIQPEETLTLDVKRQRFNRQG"
     gene            complement(3932..4564)
                     /gene="dhaL"
                     /locus_tag="ACHKTW_002891"
     CDS             complement(3932..4564)
                     /gene="dhaL"
                     /locus_tag="ACHKTW_002891"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HFO6463881.1"
                     /translation="MSLSRTQIVNWLTRCGDIFSTESEYLTGLDREIGDADHGLNMNR
                     GFSKVVEKLPAIADKDIGFILKNTGMTLLSSVGGASGPLFGTFFIRAAQATQARQSLT
                     LEELYQMFRDGADGVISRGKAEPGDKTMCDVWVPVVESLRQSSEQNLSVPVALEAASR
                     IAESAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            complement(4575..5645)
                     /gene="dhaK"
                     /locus_tag="ACHKTW_002892"
     CDS             complement(4575..5645)
                     /gene="dhaK"
                     /locus_tag="ACHKTW_002892"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415718.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HFO6463882.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYVTRADAPVAG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECAMQIDGGEGVLMI
                     IKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLDACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRPFSSLDQTVDEMFDTLLVNGSYHRTLRFWDYQQGSWQEEQQTKQPLQSGD
                     RVIALVNNLGATPLSELYGVYNRLTTRFQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDAPVHTPALNWGK"
     gene            5873..7792
                     /gene="dhaR"
                     /locus_tag="ACHKTW_002893"
     CDS             5873..7792
                     /gene="dhaR"
                     /locus_tag="ACHKTW_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415719.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase operon transcriptional
                     regulator DhaR"
                     /protein_id="HFO6463883.1"
                     /translation="MSGAFNNDGRGISPLIATSWERCNKLMKRETWNVPHQAQGVTFA
                     SIYRRKKAMLTLGQAALEDAWEYMAPRECALLILDETACILSRNGDPQTLQQLSALGF
                     NDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRLTGT
                     IALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDGVIS
                     WDEQGNLQFINAQAARVLRLDATASQGRAITELLTLPAVLQQAIKQAHPLKHVEATFE
                     SQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQDDPQT
                     RRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGDAAL
                     AEEFIGGDRTDNENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITRLDA
                     RRLIPVDVKVIATTTADLAMLVEQNRFSRQLYYALHAFEITIPPLRMRRGSIPALVNN
                     KLRSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRVSDLPE
                     HLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTLWRK
                     MKQHGIDAGQFKRRV"
     gene            complement(7892..10759)
                     /locus_tag="ACHKTW_002894"
     CDS             complement(7892..10759)
                     /locus_tag="ACHKTW_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFO6463884.1"
                     /translation="MGIKQHNGNTKADRLAELKIRSPSIQLIKFGAIGLNAIIFSPLL
                     IAADTGSQYGTNITINDGDRITGDTADPSGNLYGVMTPAGNTPGNINLGNDVTVNVND
                     ASGYAKGIIIQGKNSSLTANRLTVDVVGQTSAIGINLIGDYTHADLGTGSTIKSNDDG
                     IIIGHSSTLTATQFTIENSNGIGLTINDYGTSVDLGSGSKITTDGSTGVYIGGLNGNN
                     ANGAARFTATDLTIDVQGYSAMGINVQKNSVVDLGTNSSIKTNGDNAHGLWSFGQVSA
                     NALTVDVTGAAANGVEVRGGTTTIGADSHISSAQGGGLVTSGSDATINFSGTAAQRNS
                     IFSGGSYGASAQTATAVVNMQNTDITVDRNGSLALGLWALSGGRITGDSLAITGAAGA
                     RGIYAMTNSQIDLTSDLVIDMSTPDQMAIATQHDDGYAASRINASGRMLINGSVLSKG
                     GLINLDMHPGSVWTGSSLSDNVNGGKLDVAMNNSVWNVTSNSNLDTLALSHSTVDFAS
                     HGSTAGTFATLNVENLSGNSTFIMRADVVGEGNGVNNKGDLLNISGSSAGNHVLAIRN
                     QGSEATTGNEVLTVVKTTDGAASFSASSQVELGGYLYDVRKNGTNWELYASGTVPEPT
                     PNPEPTPAPAQPPIVNPDPTPEPAPTPKPTTTADAGGNYLNVGYLLNYVENRTLMQRM
                     GDLRNQSKDGNIWLRSYGGSLDSFASGKLSGFDMGYSGIQFGGDKRLSDVMPLYVGLY
                     IGSTHASPDYSGGDGTARSDYMGMYASYMAHNGFYSDLVVKASRQKNSFHVLDSQNNG
                     VKANGTANGLSISLEAGQRFNLTPTGYGFYIEPQTQLTYSHQNEMAMKASNGLNIHLN
                     HYESLLGRASMILGYDITAGNSQLNMYVKTGAIREFSGDTDYLLNNSREKYSFKGNGW
                     NNGVGVSAQYNKQHTFYLEADYTQSNLFDQKQVNGGYRFSF"
     gene            complement(11600..12691)
                     /gene="ychF"
                     /locus_tag="ACHKTW_002895"
     CDS             complement(11600..12691)
                     /gene="ychF"
                     /locus_tag="ACHKTW_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFO6463885.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            complement(12808..13392)
                     /gene="pth"
                     /locus_tag="ACHKTW_002896"
     CDS             complement(12808..13392)
                     /gene="pth"
                     /locus_tag="ACHKTW_002896"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFO6463886.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            13670..13948
                     /gene="ychH"
                     /locus_tag="ACHKTW_002897"
     CDS             13670..13948
                     /gene="ychH"
                     /locus_tag="ACHKTW_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HFO6463887.1"
                     /translation="MKRKNASLLGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            complement(14003..15682)
                     /gene="dauA"
                     /locus_tag="ACHKTW_002898"
     CDS             complement(14003..15682)
                     /gene="dauA"
                     /locus_tag="ACHKTW_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HFO6463888.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(15807..16754)
                     /gene="prs"
                     /locus_tag="ACHKTW_002899"
     CDS             complement(15807..16754)
                     /gene="prs"
                     /locus_tag="ACHKTW_002899"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFO6463889.1"
                     /translation="MPDMKLFAGNATPELAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            complement(16905..17756)
                     /gene="ispE"
                     /locus_tag="ACHKTW_002900"
     CDS             complement(16905..17756)
                     /gene="ispE"
                     /locus_tag="ACHKTW_002900"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFO6463890.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLIKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            complement(17756..18379)
                     /gene="lolB"
                     /locus_tag="ACHKTW_002901"
     CDS             complement(17756..18379)
                     /gene="lolB"
                     /locus_tag="ACHKTW_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein insertase outer membrane protein
                     LolB"
                     /protein_id="HFO6463891.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            18593..19849
                     /gene="hemA"
                     /locus_tag="ACHKTW_002902"
     CDS             18593..19849
                     /gene="hemA"
                     /locus_tag="ACHKTW_002902"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HFO6463892.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFADSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSEIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            19891..20973
                     /gene="prfA"
                     /locus_tag="ACHKTW_002903"
     CDS             19891..20973
                     /gene="prfA"
                     /locus_tag="ACHKTW_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFO6463893.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYSLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            20973..21806
                     /gene="prmC"
                     /locus_tag="ACHKTW_002904"
     CDS             20973..21806
                     /gene="prmC"
                     /locus_tag="ACHKTW_002904"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFO6463894.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEIIAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            21803..22195
                     /gene="sirB2"
                     /locus_tag="ACHKTW_002905"
     CDS             21803..22195
                     /gene="sirB2"
                     /locus_tag="ACHKTW_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB2"
                     /protein_id="HFO6463895.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            22199..23008
                     /gene="sirB1"
                     /locus_tag="ACHKTW_002906"
     CDS             22199..23008
                     /gene="sirB1"
                     /locus_tag="ACHKTW_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB1"
                     /protein_id="HFO6463896.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDVWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVEQCPEDPISEMIRAQINNIAHKHIVLH"
     gene            23044..23898
                     /gene="kdsA"
                     /locus_tag="ACHKTW_002907"
     CDS             23044..23898
                     /gene="kdsA"
                     /locus_tag="ACHKTW_002907"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HFO6463897.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSMMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
BASE COUNT         5876 a         5961 c         6106 g         6027 t
ORIGIN      
        1 agtcaggcgt taaaaatggg gcggatagtg aggcggtttt ggctgcgctt attgaacatt
       61 tagaattgat caatccttct ggcagattaa gccaaaatac ccgttcggca atgcttcagt
      121 tacgcgaaga ggaatggtct gaattctttt tttggttgct aaattctctg gagtgtctgg
      181 attatgtgat tattaattta actccagaaa gcaaaaaaac gttgatgagt gaacaccgta
      241 acaatattca ggtagcaatc gatgctttat ataggcaacg tcggtataaa tcaccggggg
      301 atgatagtga aacacttacc cgtagaaatg acgctatctt cggcaaccac gtatggcaaa
      361 catttgcgca atacttcccg ccaggacttg aaaaaccgtc tgtgtagaaa acaccatagc
      421 tatggtgtta tctgccgaca acgccatccg gagaagccga atgacgttgt cggagtaagc
      481 gctggttaag gtgtgggttg tgcctctttg gttgagggtt gcgtcgttgc tgacttaacg
      541 gtcggacgcg tcgccggaac attgtcacac ggttgctctt tcgggcagat caaatccagc
      601 attttcagcg tcacgccatt ggtccagcca aagccatcct gtaatggata ttcgccaccg
      661 ccgccccccg ttccggtggt gctgacatca tatttttcca ccagcttttt ctcccggtca
      721 taggtgtgct gaacattggt caggaagtgc cagctaatgt ccatcgccac ctctttttgc
      781 ccgtagtttt gtaatccttc tgtcgcgacc cactgtaacg gtgcccagcc atttggcgca
      841 tcccattgtt gcccactttt caccgacgtg gtgttcaggc cgccgggttg cagcagatgt
      901 gttttcgtcg ccgtcgccat tttgttggcg cgatctttcg ctgccgcatt gacgtacagc
      961 gggaacaggg cggccgcggt taactgattg cgcactttat gacttttcag gtcgtaatcg
     1021 gcataccagc cttgttgatc gttccacagg tatttttcga tccctttttg acgggcattt
     1081 gccagcgttt cgtactggtt tgccatcgcg ttatctccgg cagctttgct ggcgcgggcg
     1141 aggatttttt ccattttaaa catcaggctg ttcagatcga ccggtacgat gctggtggtg
     1201 cgtaaggtat ttaactgctg cgggttgtcc atccagcgcg agctgaaatc ccagccagac
     1261 gcagcggcag agcgcaggtc gcggtaaatt tcagtggcag gtcgattcgg attgcttttg
     1321 gcggtggcaa tatcttccac ccatgactct ggtcgtggcg tatcgcgatc gtcccagtag
     1381 cggttgagaa gggtaccatc ctgaagtttg acaacgcgtt tttcctgttg tccggcttgc
     1441 aggttttcaa caccgtccat ccagtaagca tattcttttt gcatttgcgg caggtattgc
     1501 ttcaacgcgg catcgccttc atgctgcgcc agtaactcta ccatcatggc aaagaagggc
     1561 ggttgcgagc ggcttaaata gtaactgcgg ttgccgttgg gaatatgacc gtaagtgtct
     1621 atttcatgag caaaattggc caccatatcc gcgactttat cccagtgacc gctttcggca
     1681 agtcctaaca tggtgaagta actgtcccag taatatacct cgcgaaaacg tccgcccggc
     1741 acgacataag gttccggcag cggtaacaga gaatcccatt tttctgtgtt ttcggtagaa
     1801 cgcgttaata ccggccaaag tccgtcaata tgttcgcgca gtgactgccc ctctggcgga
     1861 acatatttct cgccttcttt cggcagggtg aaattgacgt taacgaaatg gcgcagatca
     1921 aatccgctct ggttttgctg catccgataa tcagcaagga tcatcagcgg atcgctgttc
     1981 ggcacggcat cggcaaaggt tttttggtcc ggaaaaagtt tggcgttttg cacatcatta
     2041 aacagcggcc ctaataaaat atcaggcggc tgtggtgtta ccggtgtttc ttctgcctgc
     2101 accgatagcg cagcgaaaca caaaaagata caggctggaa ttaacgccat tttttgcggg
     2161 cgagaaggtg cgggggattt catcaatcat tctcctttgg cgaaaccgaa taaaacggta
     2221 ttcaagtatc agaaaacctt agttcaggat cgcactagct gcatgatcga aatctcattc
     2281 tgcgaactgt caggcatttt agaaaaggga attaaaggga taaataacgg cgaaatagag
     2341 tgatcaaata acgtctgttt attagccagc cagcggcgtt gggttcgtga ttagcgaaga
     2401 aaaatcatca taaattggtg aaaaatataa caggtagtta aaaaccatta gtgctgagta
     2461 aattgccgga tgacatcaga acgatgccat ccgaacagtg gcttaaccct gacggttgaa
     2521 acgttgcctt ttaacgtcca gcgttagcgt ttcttctggt tgtatcgcat acagtttctc
     2581 accctgctgg caaatccagc caatccccag ttcacgggcg attagggcgc tgtgggatac
     2641 cggacttccg gcgctaaggc agataccttt tacaaccgcc ggatccagtt gcagtactgt
     2701 ggaaggataa atgttctccg ccagtagaat agttggcgag ttaaactgcg ggagttcttc
     2761 tttcgtttgg gtcaggtgga ccagggtgcg atgcagaaga tcgtccacat caatatagcg
     2821 agcttgtaga tattcatcat ccagttgctg gtattgctgg ctaagttctt taagaacttg
     2881 ctgccaggca tattctgccg tgcaatgttc atgctgaagg agttcgcttg ccgccgccag
     2941 cagttccgga tcatctaaca gtgtatggtg accagaaaag attgcggcaa tatcgtcaag
     3001 cccgctggct tctgctttcg ctgttaacgt catcagatct aataacgtga agtcaatagc
     3061 ctggcgtaat cgatcttgtt cttcttccac ggtcagggtt gattttgcct gtaccgtaca
     3121 taaaactggt tgataataaa aggctttacc cgaaacaggc ggaacgggac gcagagtagg
     3181 tggagcgact tcctccgttt caccaaagtt atcttcagcc agctgacgga aagcgatcag
     3241 tgcctcttca gcttctggcc ctttcgcaat caggcgcagc gtatcgttat agcgaacttg
     3301 tagtaacgca atctggttaa tactctctgg tgtgacgcat ttgccgtttt tttccagcaa
     3361 catatcggca ttaaatgtcg ataaggtata aaccagccgg gaggccggac gtacatgcag
     3421 gccgttacgg ttttttatga ccaccgccag agaacgggct tcttcatcgt acgcaggaca
     3481 tgtgtcagag attttagtgt cggaggacgg taaacccagt tgttcacgtt tggcttccag
     3541 cgcatgcata gcgtcaaaga taactttgtc gatatccgct cccgaggccg cgctgaccgt
     3601 tgctgccagt gtaccttcga ccaacggcgc agcacacaaa cgtacttttg cggcgatctt
     3661 gggagccagc aattccagcg cagtttcagc actcaataat gcgctaccca tatccatcat
     3721 gaccagcaca tggtcggcat cagcaacaga ttcgatggcc tccatcactt tgacggcatc
     3781 ggtaccaata ggattttgtg gatcgtcaat tcccgcggca atggcgattt tacaactatc
     3841 actcattaac atctgacggg ctaattcacc gacaccttct cccagtcggc tgctatgtga
     3901 aactatgacc aggtttacca tcaccaattc cttactcttt tgcggctaac gccaacattt
     3961 gcatcataaa catcaccgag gtcgcgccgg gatcctggtg accaatactg cgttcaccga
     4021 gataactggc gcggcctttg cgggcttgca tcgtaatcgt actttgtgca gcggattcgg
     4081 cgattctact ggcagcttcg agcgccaccg gaacagagag attttgctcg ctggactgac
     4141 gtaacgattc caccaccggc acccacacat cacacatggt tttatcgcca ggttcggctt
     4201 tcccgcgact gattacgccg tccgcgccat cgcggaacat ctgataaagc tcttccagtg
     4261 tcaggctttg ccgtgcctgg gtcgcctgtg cggcgcggat aaagaaggta ccgaacagcg
     4321 gaccactggc accaccgacg ctggaaagca gcgtcatacc ggtattcttg agaatgaaac
     4381 cgatatcttt atctgcgata gcagggagtt tttccaccac tttgctaaag cctcggttca
     4441 tatttagccc gtggtcagcg tcgccaattt cgcgatccag tccggtaaga tactcgctct
     4501 cggtgctgaa aatatcgcca caacgagtga gccagttaac aatttgagtt ctgctcagtg
     4561 acattgcttt ctccttattt accccagtta agggccgggg tgtggaccgg ggcgtcccag
     4621 agtgccagcg tttcgtcatc aactttcagt aaggtgattg agaaaccggt catatccagt
     4681 gaggtgcagt acgcgccaat taaattacgt tcgatagtca atcccgcttg ctggaaacgt
     4741 gtggtcaggc ggttatagac gccgtacagc tcagaaagcg gagttgcgcc aagattgtta
     4801 accagcgcaa tcacccgatc gccagactgg agcggttgtt tggtttgttg ttcttcctgc
     4861 caactgcctt gttgataatc ccagaaacgc aaagtgcgat ggtatgagcc atttaccagc
     4921 agggtgtcga acatttcatc gacggtttga tcaagggaag agaaggggcg gcggtcaata
     4981 cccggctcac catgaatgcc gacgccaaac tccatctcat tatccgccag ggtaaaagaa
     5041 ggtttgcccg cggcaggaac ggtacaggca ccgagagcga tacctattga gtggccttga
     5101 ttattcaact tacgccccag ttccgcacag gcgtccagtg agtcgccacg ctccgccgct
     5161 gcgcctacga gtttttcaat taatacggtg ttggcaacgc cgcgtcgccc ggcagtataa
     5221 agactgtctt ttaccgcaac gtcgtcatca atgaccacag tggtcacttt tacgccgcta
     5281 tcgtgcagta actcggtcgc tgtttcaaag ttaagaatat cgccggtgta atttttgata
     5341 atcatcagta caccttcgcc gccatcaatt tgcatggcgc attcaaagat tttatcgggc
     5401 gtcggtgagg tgaaaatttc gcccggacag gcccccgaaa gcatcccctg accgatataa
     5461 ccacagtgca tcggctcgtg tccgctgccg ccacccgaca gcagggcgac ttttcctgca
     5521 acgggggcat cagctcgggt gacatacacc ggatcctgat gcagtgtcag agatggatgc
     5581 gctttcgcca gtcctgccag ttgttcgtcc agtacgtctt gcacatcatt gatcaatttt
     5641 ttcattattt tgctccagca attacggtag ggcatggatg atgttcaacg acacggcgac
     5701 cactgactgc cgatgaatcc attgtgcatc aggcaaggga aaagaaaatt agcccaaatt
     5761 atgtttcata gtgaaacata tgctttaatg aatgttccat attgaaactt ttacgtgtat
     5821 taatacttaa aattgcgagc cggaacacct tttgtcataa gggatgcggg atatgagtgg
     5881 cgcttttaac aacgatggtc ggggcatatc tcccttaatt gcaacctcct gggagcgatg
     5941 caataagctg atgaaacggg agacatggaa cgtaccacat caggcccagg gcgtgacatt
     6001 tgcttctatt tatcggcgta agaaagcgat gctgacgctc ggtcaggctg cgctggaaga
     6061 tgcctgggaa tatatggcac cgcgagagtg tgcgctgctt atcctcgatg aaaccgcctg
     6121 cattctcagc cgtaatggcg atccgcaaac cttgcagcag ctaagtgcac tgggattcaa
     6181 tgacggcacg tattgcgccg agggaattat tggtacttgt gcgctatcgt tagcggctat
     6241 ctctggtcag gccgtgaaaa cgatggccga tcaacatttc aaacaggcac tctggaactg
     6301 ggccttttgt gcaacgccgt tgtttgacag caagggccga ttgacgggaa caatagcgct
     6361 ggcgtgtccg gttgagcaaa ctaccgcagc tgatttgccg ttgacgttgg caatcgcccg
     6421 cgaggtcggt aatttactgc tgacggacag tttgctcgct gaaactaacc gtcatttaaa
     6481 tcaacttaat gccctgttag aaagtatgga tgatggcgtg attagctggg acgagcaggg
     6541 taatttgcaa tttattaatg cccaggcggc gcgggtcttg cgccttgacg cgacggcaag
     6601 tcagggacgg gcaatcactg aactcttaac gttacccgcc gtattgcaac aagcaataaa
     6661 acaggcacat ccgctcaaac acgtagaagc aacctttgaa agccagcacc agtttattga
     6721 tgcggtgata acccttaaac cgataataga aacgcaggga accagcttta ttttgttgct
     6781 ccatcctgtg gaacagatgc ggcagttgat gaccagtcaa ttaggaaaag tcagccatac
     6841 ctttgctcat atgccacagg acgatccgca aacccgccgc ttgattcatt ttggtcgcca
     6901 ggcggcgcgc agtagctttc ctgtcctgct ttgtggagaa gagggcgtgg gcaaggcact
     6961 gctaagtcag gcaattcata atgaaagcga gcgtgctgca ggtccttata tcgccgtcaa
     7021 ttgtgagtta tatggtgatg ctgcgctggc ggaagaattt attggtggcg atcgcacgga
     7081 caatgaaaat ggccgtctga gtcggctgga actggcgcac ggcggcacgc tgtttcttga
     7141 aaagattgaa tatctggcgg tggagttaca gtctgctttg cttcaggtta tcaagcaggg
     7201 tgttatcacg cgactggatg cgcggcgttt aataccagtt gatgtcaaag tgattgccac
     7261 aacgaccgcg gacctcgcaa tgctggtgga acaaaatcgt tttagtcgcc agctgtatta
     7321 cgcgttgcat gcatttgaaa ttaccatccc gcctctgcgt atgcggcgtg gcagcattcc
     7381 ggcgctggtg aataacaaat tacgcagtct tgaaaaacgc ttctctacgc ggctgaaaat
     7441 tgatgacgat gccctcgctc gcctggtttc ctgtgcatgg cctggcaacg attttgaact
     7501 ttacagcgtc atcgagaatc ttgctctgag tagtgataat gggcgcattc gcgtcagtga
     7561 tttgccggaa catctgttta ccgagcaggc aacagatgat gtcagcgcca cacgcctttc
     7621 caccagtctg tcatttgcgg aagttgaaaa agaggcaatt attaacgcag cccaggtcac
     7681 aggcggtcgc attcaggaaa tgtcggcttt acttgggatc ggccgcacta cgctgtggcg
     7741 gaaaatgaag caacatggca ttgatgcagg gcagtttaag cgccgggtat gaaagacaga
     7801 gacgatttct gatacatcag agtgatctat atttcattcc ggcgcacgct aacatttttc
     7861 agcatcgttt aagggctggt ctatcccgca cttaaaagct gaagcgatat cctccgttga
     7921 cttgcttctg atcaaagagg ttactctgcg tgtaatccgc ttcgaggtag aatgtgtgct
     7981 gtttgttata ctgtgcactg actcccacgc cgttattcca gccattacct ttgaaactgt
     8041 acttctcccg ggagttgttc aacagatagt ctgtatcccc tgaaaactcg cggatagctc
     8101 cagtcttcac atacatattc agctggctgt tgcctgcggt gatgtcatat cccagtatca
     8161 tgctggcacg ccccagcagc gattcgtagt gattcagatg tatattgaga ccattactcg
     8221 ccttcatagc catctcattc tggtggctgt atgtgagctg ggtttgcggc tctatataga
     8281 acccataacc agtaggggtc aggttgaacc tctgcccggc ttccagggag atgctcaatc
     8341 cattcgcagt gccgttggct ttaacgccgt tgttctgact gtccagtacg tgaaaactat
     8401 ttttctggcg cgatgctttt acaacgagat cgctgtaaaa accgttgtgt gccatgtaac
     8461 tggcatacat tcccatgtag tctgaacgtg cggtaccgtc gcctccgcta tagtccggcg
     8521 atgcatgtgt tgagccaata tacagaccga catacaacgg cattacatca gagagacgtt
     8581 tatccccacc aaactggata ccgctgtaac ccatgtcaaa gccgctcagt ttgccactgg
     8641 caaaggagtc caggcttccc ccataactgc gcaaccagat attaccgtct ttactctgat
     8701 ttcgcaggtc acccatccgt tgcatcagcg tacggttttc aacatagttc aataagtaac
     8761 cgacattgag ataattgccg ccagcatctg cggtcgtggt gggtttaggc gtgggagcgg
     8821 gttcaggcgt aggatcgggg ttgactatgg gaggctgagc gggagctggt gtgggttcag
     8881 gattaggagt cggttccgga actgtcccgg aagcgtaaag ctcccagtta gtgccatttt
     8941 tacgcacatc gtacagatat ccccccaact caacctgaga agacgcgctg aacgaggccg
     9001 cgccgtcagt ggtttttacc actgtcagaa cttcatttcc cgttgtggcc tcgctgccct
     9061 ggttgcggat agccaatacg tgattaccag cactactccc gctgatattc aataaatccc
     9121 ctttattatt aacgccatta ccctcgccaa caacatcagc acgcataata aaggtactgt
     9181 taccgctcag gttctctacg tttaatgtgg caaatgtgcc ggcagttgac ccgtggctgg
     9241 caaaatcgac agttgaatgg ctcagcgcca gcgtgtcgag attagaatta cttgttacgt
     9301 tccagacgct gttattcatt gcaacgtcca gttttccgcc attgacatta tcgctgaggg
     9361 aggaacctgt ccaaaccgac ccagggtgca tatccagatt gattagccca cctttggaaa
     9421 gaacgctacc gttgataagc atacgacccg aggcgttgat gcggctggcg gcataaccat
     9481 cgtcatgttg cgttgcaatg gccatctggt cgggtgtact catatcaatg accagatcgc
     9541 tcgtgaggtc gatctggctg ttggtcatgg cataaattcc cctggctcct gccgcgccgg
     9601 tgatagccaa actgtctccg gttattctac cgccgctgag cgcccacaaa cccagcgcca
     9661 gactgccatt acgatcaact gtaatatcgg tattttgcat gttgacaaca gccgttgccg
     9721 tctgggccga ggcaccataa gaaccgccgg aaaagatgct gtttcgttgc gccgccgtgc
     9781 cagaaaaatt gattgtcgcg tctgaaccac tggtgacgag gccaccgccc tgcgcggaag
     9841 aaatatggct atctgcaccg atagtggttg taccaccacg aacttcgacg ccattggccg
     9901 cggctccagt tacatcaaca gtgagtgcat tcgcgctaac ctggccaaag ctccagaggc
     9961 cgtgtgcatt atcgccattg gttttaatgg aactgtttgt tccgagatcg acaacagagt
    10021 ttttctgtac gtttatcccc atggcgctgt agccctgaac atcgattgtc aggtctgtag
    10081 ccgtaaaacg cgcagcacca ttggcgttat tgccgttgag accaccgata taaacacctg
    10141 tacttccatc ggtcgtgatt ttacttccgc ttccaagatc gacactggtg ccatagtcat
    10201 tgatggttag gcctataccg ttcgagtttt caatggtgaa ttgagtggct gttagtgttg
    10261 agctatgccc aataatgatg ccgtcatcgt tactcttaat ggtgctgcct gtgcctaagt
    10321 cagcatgggt atagtcacca attaagttaa tgccgatggc agaggtttga ccaacaacat
    10381 ctactgtgag tcggttagct gtcagggagc tgtttttgcc ctgaataatg attccttttg
    10441 catatccaga ggcgtcgttg acattgacgg tgacatcatt acccaggttg atattgccag
    10501 gcgtgtttcc tgctggggtc attacaccat agaggtttcc tgatggatcg gcggtatctc
    10561 ctgtaattct gtcaccatca ttaatagtaa tattggtgcc atattgactt cctgtatcag
    10621 cagctatcag caggggggaa aagataattg cattcaaacc aatagcgcca aattttatca
    10681 gttgaattga gggcgaacgg atttttaatt cagcgagacg atctgctttg gtattcccat
    10741 tgtgttgttt gatgcccata ccactcctat atagtaccca gactatgggt ctatggattt
    10801 tgtcattttg tttaaatcaa tgaataatat cctcttatca tcatgatatt tgtatggtgc
    10861 aatcatgatg agcttctcag agaataattc tctattaccg ggcaaaatca ggaacacagg
    10921 aattaatata tatttaacat cccccttaca aaggtgggtt tatatcgagt tattccctaa
    10981 gttgtcgttg cttattccaa ttcgttacta taaatatggc aaaaatatta caacaaacag
    11041 cggttaaaaa atttggtgaa gattttttgt aataatccta attagttatg ggaatttcgc
    11101 ttgatgtatc tgcataacaa aaacaatatg cgccattttt gcattactaa taagaaaaag
    11161 caaaaaacat cgatattcat ttttggtcaa tgggttttct ggcctatcgt tgttattgtg
    11221 ctactggtta tcattttcat cttgccagga tcgcacgtaa tcacgttatt cggggacttt
    11281 atctgcggga tattaccctt caggtaagtc cttttctcgc cagcatattg gagcgtgatc
    11341 aatattgatc agctgtgaag ggggtgatgt gtagtggacc aaaacttacg ttatatcact
    11401 actaatgaga tatccgtcat ctgacactga tgtctttaat tattctcgtg gttcactgta
    11461 ggcaataagc acaaaagtgt aggatgttac aagaatgatt aggactcggt gaaatgaaaa
    11521 atccacgcaa ttgcgtggat tttatatact tttgcgctct tcatgagatt tagcgaaacc
    11581 tcatgagaca ataaattaat tagacgttga aaaggaagtt catcacatcg ccatctttca
    11641 cgatgtaatc tttaccttct gcacgcattt tgcctgcttc tttcgcgcct tgttcacctt
    11701 tgtaagtgat gaaatcttca aacgagatgg tttgtgcacg gataaagcct ttttcaaaat
    11761 cagtatggat tttgcccgct gcctgcggcg cggttgctcc aaccggaatg gtccatgcac
    11821 gcacttcttt caccccagcg gtgaagtaag tttgcaggtt cagcagctta taaccggcac
    11881 ggatcacacg gttcaggccc ggctcttcca gcccaagctc ctgcataaac tcgtcacgtt
    11941 cttcgtcgtc cagttcggca atgtctgctt caacagcagc acaaaccgga accacaacag
    12001 aaccttcttt cgccgcgatt tcacgcacct ggtcaagata tgggttgttt tcaaaaccgt
    12061 cttcgttgac gttggcgatg tacattgttg gtttcagcgt caggaagctc aggtaacgaa
    12121 tagccgcttt ctcttcagcg cttaaatcca gcgcgcgcag catacctgca ttttccaact
    12181 ggggcaggca tttttccagg accgccagct cagctttcgc gtctttatcg ccacctttgg
    12241 ctttcttctg tacgcgatga atcgcacgtt cgcaggtgtc gaggtctgcc agcgccagtt
    12301 cggtgttgat aacttcaata tcgtcagccg ggttaacttt gccggaaacg tgaatgatgt
    12361 tgtcattttc aaagcagcga acaacgtgac cgatcgcttc ggtttcacgg atgttggtca
    12421 ggaactggtt acccagacct tcgcctttcg atgcgccttt taccagaccg gcgatatcga
    12481 caaattccat ggtcgtggga agcgtacgtt gcggttttac gatttcagcc agttgatcca
    12541 ggcgagggtc aggcattggt acgacgcctg tgttcggctc aatggtgcag aatggaaagt
    12601 tggccgcttc aataccggct ttggtcagcg cgttgaacag ggtagatttc ccgacgttgg
    12661 gcaaaccgac gataccgcat ttgaatccca tgatttaaat caccttaata tcttaataat
    12721 caacctgtta ttgctaacag attgcagaaa tggaaataac tttgcctatt atacacggca
    12781 ctcggcaaaa atgccgcaga caacgactta ttgcgcttta aaggcgtgca atcggttcgt
    12841 tgctttggtc aagccatctg taaaccacat ttcagtacaa cgcgccgctt cgtcgatggc
    12901 ttcatcaatt aacttctgtt cactaacagg cggtttgcct aacacaaaac cgacaacttt
    12961 atttttatcg cccggatgac cgattccgat gcgtaaacgg tgaaagttag ggttattacc
    13021 caatttactg atgatgtctt tcagtccatt gtgaccacca tggccaccgc ccaatttaaa
    13081 tttggcgacg ccaggaggca gatccagttc gtcgtgggcc accagaattt cgtccggatt
    13141 aatgcggaaa aaactggcca tcgccgcaac ggctttgccg ctgagattca taaatgtagt
    13201 cgggactaac aggcggacat cttcgcctcc aagagtgact cgcgaagtat aaccaaagaa
    13261 tttagcctct tcgcgcagcg gagcgcgcaa acgctctgcc agtaagtcaa cgaaccaggc
    13321 accagcatta tgtcgcgttg cggcgtattc agcaccgggg ttcgccaggc cgacaatcaa
    13381 tttaatcgtc acgttttttt gtcctgagtg tgtacataac tggcgcgtag tttactggtt
    13441 gcggccccgc ttgacaaaaa actgcgtatc aaatgcagat aacgtaataa ttgcctgagt
    13501 ggactattag aaagtcaagg tgttcaggcg tttatttgta aagttttgtt gaaataaggg
    13561 ttgtaattgt gatcacgccc tcacataacc cacagggtgt tgtctatact ttacacataa
    13621 ggaagagggg tattccctgt tacaacccag aaagttccgg aggtgacata tgaaacgcaa
    13681 aaacgcttcg ttactcggta acgtgctcat ggggttgggt ctggtggtaa tggtggtcgg
    13741 cgtggggtat tcaatcctca accagttacc acagtttaat atgccccagt atttcgcaca
    13801 tggtgcagtg ctaagtattt tcgtcggtgc cattctctgg ctggcgggtg cccgtgttgg
    13861 cgggcatgaa caggtgtgcg accgttactg gtgggttcgc cactatgaca aacgttgccg
    13921 ccgtagcgat aatcgccgtc atagctaaca aataatgcag tttgctgacc agtcagtttg
    13981 cgctgactgg tcaatctcgt acttataaat ctgccatcgc cgcgcgacga ttcgggaaga
    14041 acgccaggcg tcccgggatc ggttgaatgc cagcgcgagc catagtgcgc agtggctgga
    14101 attccacgtt gcacacgcgc agttcacatc cctcgggcag acgcttcaca aaacgctgga
    14161 acgcatcaag accaccagca tcaagtaccg gaaccgcatc ccacttcaga atcacaatcc
    14221 gtttgccttc aagacgtgac tccaggtccg tgaacaagcc ttctgcagca gcaaaaaaca
    14281 gcgggccaat aacgcgcagt accagaacat cgtctggaac atctacgact accggtgcca
    14341 ggcgagtcat acgtgcgata cgacgcataa acagcagcga tgccagcacg atccccacgc
    14401 tgatggcaat aaccatatca aacagcacgg tcagcgacat gcacagcagc atgacaatga
    14461 tgtcatcttt cggtgcatga cgcagcaagt cgaccacttt atgcgcttca ctcatgttcc
    14521 acgccaccat caacagcagg gctgccatag cggaaagcgg cagccaggag agcagcggtg
    14581 ccagtaccag cagggcaaga ataaccagaa tagagtggat caccgctgag ataggggaag
    14641 ttgccccggc acggacgtta gcggcagaac gcgcgatggc agctgtagcg gtaataccac
    14701 caaagaacgg agcgataata ttccccagtc cctgtccaac cagttcgctg ttcgccttgt
    14761 gtttcgtccc ggtcatacca tccagtacca cggcgcagag cagagattcg attgcgccga
    14821 gcattgccat tgagaatgcc gcaggcagca gtgtgcgaat agaatcccag gttagcgtga
    14881 attctgaatt aggcagatcc cacggcagca ccagttgcgg cagcagttgc ggaataccgt
    14941 taccctgaga accatcggcc aggacgtagt ggaattgcga accgatggta gcaacatgtc
    15001 cgccgagcag gttaacaatc cccatcaccg cgcaaccagc cagcaaggcc ggaaggtgac
    15061 cgggtaaacg aatgcccaga cgcggccaga aaacaagaat acctagcgtc acaatgccaa
    15121 tggcagcatc acccacatta atggtcggca gcgccataaa taatgcgccg actttttgta
    15181 gataatgttc cgggacatgg gccatttgca gaccgagaaa atctttaatc tgcatggtac
    15241 cgatggtgat cccgataccc gaggtgaaac ctaaggtgac ggaaaccgga atatactcaa
    15301 tcaggcgacc aaagcgtgcc agacccatca gaatcaaaaa aatccccgac agcaaggtcg
    15361 caaccagcag tcctgccagt ccaaactgtt gcgacacggg atagagaatt accacaaatg
    15421 ccgcagtcgg accggaaacg ctaaagcgtg acccacccgt cagagcaatg acaatccccg
    15481 caacagctgc ggtatataaa ccgtactggg gtgccacacc actaccaata gccaacgcca
    15541 tcgccagcgg gatagcaata atcccgacgg ttatcccggc aatcaggtca cgggtaaacc
    15601 gtgcggcagt atatttttct ttccagcaag cgtcgatcag agcgcggaaa ggcatcacat
    15661 gtgaggaaaa tattttgttc acaataatgt ttcatccgtg agcgcatcat ctgtcaacta
    15721 aatggcaggt gaaggaggca taggtcatac aaatggatat tacagacaaa aaaacccgcc
    15781 gcagcgggtc tttgagccgg gttcgattag tgttcgaaca tggcagagat cgattcttcg
    15841 ttgctgatac gacgaatcgc ttcggccagc atacctgaca gggtcagagt acgcacgttc
    15901 ggcagtgatt tgatttcatc gctcagcgga atggtatcgc agacaacgac ttcatcaatt
    15961 acagagttac gcaggttatt cgccgcgttg ccagagaaga tcgggtgagt cgcgtacgca
    16021 aatacacgtt tagcaccacg ttctttcaga gcttcagcag ctttacacag cgtaccgcca
    16081 gtgtcgatca tatcatcgac cagtacgcag tcacgacctg caacgtcacc gatgatatgc
    16141 atcacctggg aaacgttcgc acgcggacga cgtttgtcga tgattgccat atcggtatcg
    16201 ttcagcagct tagcgatagc gcgggcacgc acaacgccgc cgatgtccgg agaaaccaca
    16261 attgggttat ccagattcag ctgcagcatg tcttccagca ggatcgggct accaaatacg
    16321 ttatcaaccg gaacgtcgaa gaaaccctga atctgttcag cgtgcagatc cactgtcagc
    16381 acacggtcaa caccgacgct ggagaggaag tctgcaacca ctttcgcagt gattggtaca
    16441 cgagcggaac ggacgcgacg gtcctggcgc gcatagccaa agtaggggat aacagcggtg
    16501 atacgacctg cggaagcacg acgcagggca tcaaccataa cgactaattc catcaggttg
    16561 tcgttagtag gggcacaagt ggactggatg atgaaaatat caccaccgcg tacattttcg
    16621 ttgatttgta cactgacttc gccgtcgcta aagcgaccta cagcggcgtc gccgagtgaa
    16681 gtgtacaggc ggttggcaat acgttgtgct agttccgggg tggcgttacc agcaaaaagc
    16741 ttcatatcag gcacgagaag aacctcaggc atgcgtccat tggtggaaag aatctgccga
    16801 aaactgtgcg ggccaggcat gatcctttcc aggcggtgta ttaaagagcg cgatgcaacg
    16861 tctggaacaa ggtgacgttg tcaccgaaac tcagcttgcc cggcttaaag catggctctg
    16921 tgcaatgggg aaagattaac gcctttcgcc acaaagccat tgagccattc cggggcttgc
    16981 tctagcacct ggcgggcttc agactctgta tcaaattcag caaagacaca ggcccctgtc
    17041 ccagtcaggc gcgacggggc gtattctaac agccaggaaa gcaccgcatc aacctcgcga
    17101 aaacgttttc ttgcgataac ctcgcaatca ttgctgaatt cacattttag caacgtttct
    17161 attgaccttt ttggcgtatt gcgcgggagt tcaggatctt taaaaatcac cggagtcgga
    17221 atacttacac cagggtgcgc caccagatac cacttctctg gcggatccac cggcgttagt
    17281 atttcaccaa cgccttcggc aaacgcggca tgcccccgaa caaagacagg aacatctgcg
    17341 cccagcgtca gccccatttc cgccagctca tccatgctta gcccgcattg ccagagatga
    17401 tttaatgcca ccaggaccgt cgcggcattg gatgaaccac cgccgagacc gccgcccatc
    17461 ggcaaacgct tgtcaatgct gatattcgca ccgcttcccg tcggaagacg cccgctgtct
    17521 gccgcagttt ttatcaacaa tcgcgctgcg cgaacgatca ggttatcttc atgttccacg
    17581 ccttcaacgg gcgttaacag acgaatatcc ccatcgtcac gaagctcaat gctgatggtg
    17641 tcgccgtaat caagaaactg aaacagcgtt tgcagcgtgt ggtaaccatc cgcacgctga
    17701 ccggtaatgt ataaaaacag attaagtttt gccggagagg gccactgtgt ccgcattatt
    17761 tcactatcca gttatccatt tttaacttga tgcgttgacc accgtcggtg agttccatat
    17821 tggctggcat cgcaggttgc gttttggtgt cataaccacc ataaacaacc ttccagtttt
    17881 tgccattctg gctgtaggta atttcgctca ggcggtactg gtcgtccagt ttgtagtcgg
    17941 ttgcatcacc cggtaaacct aaaatccact ggcgcaagct gttgagcgga attggcattc
    18001 cggtcaattt gccaatcatc tcttcggcgt catcggcggt ataacgctga cctttattgt
    18061 cgactaactg cacgttaccc ggttgagcat tcagctccag ttccgtgctg cccaatgggt
    18121 tagtgagcag cagacggtag cgatcctggc cggtttgctg ccagaaaaag cgggcgtaca
    18181 ctttttgttg gtcagaaata taagcgaacg cgccgcgagt ctgatactga ttaagattgc
    18241 gcacgtcttg ctgatgctga cgccattgtg gcgaatccgg gcttttgcca ggacctttgg
    18301 gcgtggtaac ggaacaggca gtgagcacaa gagcagccag cggtagcagg cggataagac
    18361 gaaaatcggg caggggcata gtgatgacaa gtccttgaga tacgttgcag ttataaccct
    18421 taatgctagc gttaccgtcc gctatcgtct atgttcaagt tgtcttaatt gccagaatct
    18481 aacggctttc ggcaattact ccaaaagggg gcgctctctt ttattgatct tacgcatcct
    18541 gtatgatgca agcagactaa ccctatcaac gttggtatta tttcccgcag acatgaccct
    18601 tttagcactc ggtatcaacc ataaaacggc acctgtatcg ctgcgagaac gtgtatcgtt
    18661 ttcgccggat aagctcgatc aggcgcttga cagcctgctt gcgcagccga tggtgcaggg
    18721 cggcgtggtg ctgtcgacgt gcaaccgcac ggaactttat cttagcgttg aagagcagga
    18781 taacctgcaa gaggcgttaa tccgctggct ttgcgattat cacaatctta atgaagaaga
    18841 tctgcgtaaa agcctctact ggcatcagga taacgacgcg gttagccatt taatgcgtgt
    18901 tgccagcggc ctggattcac tggttcttgg ggagccgcag atcctcggtc aggttaaaaa
    18961 agcgtttgcc gattcgcaaa aaggtcatat gaaggccagc gaactggaac gcatgttcca
    19021 gaaatctttc tctgtagcga aacgcgttcg cactgaaaca gatatcggtg ccagtgctgt
    19081 gtctgtcgct tttgcggctt gtacgctggc gcggcagatc tttgaatcgc tctctacggt
    19141 cacagtgttg ctggtaggcg cgggcgaaac catcgagctg gtagcgcgtc atctgcgcga
    19201 acataaagta cagaagatga ttatcgccaa ccgcactcgc gaacgtgccc aaattctggc
    19261 agatgaagtt ggcgcggaag tgattgccct gagtgagatc gacgaacgtc tgcgcgaagc
    19321 cgatatcatc atcagttcca ccgccagccc gttaccgatt atcgggaaag gcatggtgga
    19381 gcgcgcatta aaaagccgtc gcaaccaacc aatgctgttg gtggatattg ccgttccgcg
    19441 cgatgttgag ccggaagttg gcaaactggc gaatgcttat ctttatagtg tggacgatct
    19501 gcaaagcatc atttcgcaca acctggcgca gcgtaaagcc gcagcggttg aggcggaaac
    19561 tattgtcgct caggaaacca gcgaatttat ggcgtggctg cgagcacaaa gcgccagcga
    19621 aaccattcgc gagtatcgca gccaggcaga gcacgttcgc gatgagttaa ccgccaaagc
    19681 gttagcggcc cttgagcagg gcggcgacgc gcaagccatt atgcaggatc tggcatggaa
    19741 actgactaac cgcttgatcc atgcgccaac gaaatcactt caacaggccg cccgtgacgg
    19801 ggataacgaa cgcctgaata ttctgcgcga cagcctcggg ctggagtagc agtacatcat
    19861 tttctttttt tacagggtgc atttacgcct atgaagcctt ctatcgttgc caaactggaa
    19921 gccctgcatg aacgccatga agaagttcag gcgttgctgg gtgacgcgca aactatcgcc
    19981 gaccaggaac gttttcgcgc attatcacgc gaatatgcgc agttaagtga tgtttcgcgc
    20041 tgttttaccg actggcaaca ggttcaggaa gatatcgaaa ccgcacagat gatgctcgat
    20101 gatcctgaaa tgcgtgagat ggcgcaggat gaactgcgcg aagctaaaga aaaaagcgag
    20161 caactggaac agcaattaca ggttctgtta ctgccaaaag atcctgatga cgaacgtaac
    20221 gccttcctcg aagtccgagc cggaaccggc ggcgacgaag cggcgctgtt cgcaggcgat
    20281 ctgttccgta tgtacagccg ttacgccgaa gcccgccgct ggcgggtaga aatcatgagc
    20341 gccagcgagg gtgaacatgg tggttataaa gagatcatcg ccaaaattag cggtgatggt
    20401 gtgtatggtc gtctgaaatt tgaatctggc ggtcatcgcg tgcagcgtgt tcctgctacg
    20461 gaatcgcagg gtcgtattca tacttctgct tgtaccgttg cggtaatgcc agaactgcct
    20521 gacgcagaac tgccggacat caacccagca gatttacgca ttgatacttt ccgctcgtca
    20581 ggggcgggtg gtcagcacgt taacaccacc gattcggcaa ttcgtattac tcacttgccg
    20641 accgggattg ttgttgaatg tcaggatgaa cgttcacaac ataaaaacaa agcgaaggcg
    20701 ctttcagtgc tcggtgctcg catccacgct gctgaaatgg caaaacgcca acaggccgaa
    20761 gcgtctaccc gtcgtaacct gctggggagt ggcgatcgca gcgaccgtaa ccgtacttac
    20821 aatttcccgc aggggcgcgt taccgatcac cgcatcaacc tgacgctcta cagcctggat
    20881 gaagtgatgg aaggtaagct ggatatgctg attgaaccga ttatccagga acatcaggcc
    20941 gaccaactgg cggcgttgtc cgagcaggaa taatggaata tcaacactgg ttacgtgaag
    21001 caataagcca acttcaggcg agcgaaagcc cgcggcgtga tgctgaaatc ctgctggagc
    21061 atgttaccgg cagagggcgt acttttattc tcgcctttgg tgaaacgcag ctgactgacg
    21121 aacaatgtca gcaacttgat gcgctactga cacgtcgtcg cgatggtgaa cccattgctc
    21181 atttaaccgg ggtgcgagaa ttctggtcgt tgccgttatt tgtttcgcca gcgaccttaa
    21241 ttccgcgccc ggatacggag tgtctggtgg agcaggcact ggcgcggttg cctgaacaac
    21301 cttgccgtat tcttgatctc gggacgggta ccggggcgat tgcgcttgcg ctggctagcg
    21361 agcgcccgga ctgcgaaatt atcgctgtag atcgtatgcc tgatgctgtc tccctggcac
    21421 aacgtaatgc ccagcatctg gcgatcaaaa atatccacat tctgcaaagc gactggttta
    21481 gcgcgctagc cgggcagcag tttgcgatga ttgtcagcaa tccgccgtat attgacgagc
    21541 aggacccaca tcttcaacaa ggcgatgtcc gctttgagcc gctcactgcg ctggttgcgg
    21601 cagacagtgg aatggcagac atcgtgcata tcatcgaaca gtcgcgtaac gcgctggtat
    21661 ccggcggctt tctgcttctg gaacatggct ggcagcaggg cgaagcggtg cgacaagcat
    21721 ttatcctcgc ggggtatcat gacgtcgaaa cctgccgtga ctatggtgat aacgagcgcg
    21781 taacgctcgg ccgctattat caatgacaag tttttctaca ctgcttagtg ttcatcttat
    21841 tagtatcgcg ctttctgttg ggctattaac cttacgtttc tggctacgtt atcagaagca
    21901 tccacaggca tttgctcgct ggacgcgcat tgtgccgccg gttgtcgata cggtgttatt
    21961 gttaagcggc attgcgttga tggctaaagc gcacatcctg ccattttccg ggcaggcaca
    22021 gtggctgact gaaaagctgt ttggagttat catttatatc gttttgggtt ttattgcact
    22081 cgattatcgt cgtatgcaca gtcagcaggc gcgcattatt gccttcccgc tggcgttggt
    22141 ggtgctgtac atcatcatta aactcgccac cacaaaagta ccgttactgg ggtaagtcat
    22201 gagatcgtta gctgatttcg aatttaataa agcgccattg tgcgaaggca tgatcctggc
    22261 ttgcgaagca atccgccgcg attttccctc gcaagatgtt tacgacgaac tggagcgtct
    22321 cgttagtctg gcgaaggaag aaatcagcca gcttctgcct ttagaagagc agctggaaaa
    22381 actgatcgcg ctgttttacg gcgactgggg atttaaagcc tcacgcggcg tttatcgtct
    22441 ttccgatgca ttatggctgg accaggtgtt aaagaatcga cagggcagtg cggtatcatt
    22501 aggtgcggtt ttattatggg tcgcgaatcg tctcgatttg ccgctgctgc cggtgatttt
    22561 ccctacgcag ctgatattgc gcattgaatg tccggatggc gaaatttggc tgattaatcc
    22621 ttttaacggt gaatcgttaa gcgaacatat gctggacgta tggttaaagg gaaatatcag
    22681 cccgtcggcg gaactgtttt atgaagacct tgatgaagct gataacattg aggtaatccg
    22741 caaattgctg gatacactca aagcctcgtt gatggaagaa aatcagatgg agctggcgtt
    22801 acgcaccagc gaagctttat tacaattcaa ccctgaagat ccctatgaaa ttcgcgatcg
    22861 cgggttgatt tatgcgcaac tggattgcga acacgttgcg ttgaacgatt taagttattt
    22921 cgttgaacag tgtccggaag acccgatcag cgaaatgatc cgtgcgcaaa taaataacat
    22981 cgcgcataaa catattgtgc tgcattaatt aatcgacatt ttactcaaga ttaaggcgat
    23041 cctatgaaac aaaaagtggt tagcattggc gacatcaacg tagcaaatga cctgccgttc
    23101 gtactgtttg gcggtatgaa cgtgctggaa tctcgcgatc tggcgatgcg catttgcgag
    23161 cactacgtaa ctgtgaccca gaaactgggt atcccttacg tgttcaaagc ctcttttgac
    23221 aaagccaacc gctcctccat ccactcttat cgtggaccgg gcctggaaga agggatgaaa
    23281 atcttccagg agttgaagca gacttttggc gtgaaaatta tcaccgacgt tcacgaacca
    23341 agtcaggcac agcccgttgc tgatgtcgtg gatgtgattc agttgccggc gtttcttgct
    23401 cgccagactg acctggttga agccatggcg aaaaccggtg cggtaattaa cgtcaagaaa
    23461 ccacagtttg tcagcccggg acagatgggt aatatcgttg ataaattcaa agaaggcggc
    23521 aacgaaaaag tgattctttg cgatcgtggt gctaacttcg gctatgacaa cctggttgtc
    23581 gatatgctgg gcttcagcat gatgaagaaa gtgtctggta actcgccggt gattttcgac
    23641 gtgacccacg cactgcaatg ccgcgatccg tttggcgcag cttccggtgg tcgtcgtgct
    23701 caggtggctg agctggcacg agccggtatg gcggtaggtc tggcggggct gtttattgaa
    23761 gcgcatccgg atccggaaca tgcgaaatgt gatggtccat ccgcgctgcc gctggctaaa
    23821 ctggaaccgt tcctcaagca gatgaaagcg attgatgatc tggtgaaagg tttcgaagaa
    23881 ctggatacca gcaagtaatc ttttttgctt gaaaaataaa gtcttagcgt tctgcgttaa
    23941 gacttttttc atgggtgcca gatacaaaaa
//
LOCUS       DAZFQD010000073        23934 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.073, whole genome shotgun sequence.
ACCESSION   DAZFQD010000073 DAZFQD010000000
VERSION     DAZFQD010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23934
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.073"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            355..4815
                     /gene="gltB"
                     /locus_tag="ACHKTW_002908"
     CDS             355..4815
                     /gene="gltB"
                     /locus_tag="ACHKTW_002908"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HFO6463898.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPHIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHPGKALYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            4828..6246
                     /gene="gltD"
                     /locus_tag="ACHKTW_002909"
     CDS             4828..6246
                     /gene="gltD"
                     /locus_tag="ACHKTW_002909"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HFO6463899.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGSDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            6430..7557
                     /gene="yhcG"
                     /locus_tag="ACHKTW_002910"
     CDS             6430..7557
                     /gene="yhcG"
                     /locus_tag="ACHKTW_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDDEXK nuclease domain-containing protein"
                     /protein_id="HFO6463900.1"
                     /translation="MESLSERTSTGYQQIHDGIIHLVDSARTETVRSVNALMTATYWE
                     IGRRIVEFEQGGEARAAYGAQLIKRLSKDLSLRYKRGFSAKNLRQMRLFYLFFQHVEI
                     RQTVSGELTPLGIPQTPSAEFPSAKIWQTLSAKSFPLPWSTYVRLLSVKNADARSFYE
                     KETLRCGWSVRQLERQIATQFYERTLLSHDKSAMLQQHAPTETHILPQQAIRDPFVLE
                     FLELKDEYSESDFEEALINHLMDFMLELGDDFAFVGRQRRLRIDDNWFRVDLLFFHRR
                     LRCLLIVDLKVGKFSYSDAGQMNMYLNYAKEHWTLPDENPPIGLVLCAEKGAGEAHYA
                     LAGLPNTVLASEYKMQLPDEKRLADELVRTQAVLEEGYRRR"
     gene            complement(7617..8081)
                     /gene="nanQ"
                     /locus_tag="ACHKTW_002911"
     CDS             complement(7617..8081)
                     /gene="nanQ"
                     /locus_tag="ACHKTW_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate anomerase"
                     /protein_id="HFO6463901.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSTIDNEQAIILKPGMFAVFMPGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(8078..8953)
                     /gene="nanK"
                     /locus_tag="ACHKTW_002912"
     CDS             complement(8078..8953)
                     /gene="nanK"
                     /locus_tag="ACHKTW_002912"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HFO6463902.1"
                     /translation="MTTLAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEYQALEGDITDMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELAGADARTIFTRAGQGDEQAQQLIHRSA
                     RTLARLIADIKATTDCQCVVVGGSVGLAEGYLALVETYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(8950..9639)
                     /gene="nanE"
                     /locus_tag="ACHKTW_002913"
     CDS             complement(8950..9639)
                     /gene="nanE"
                     /locus_tag="ACHKTW_002913"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFO6463903.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSDAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(9687..11177)
                     /gene="nanT"
                     /locus_tag="ACHKTW_002914"
     CDS             complement(9687..11177)
                     /gene="nanT"
                     /locus_tag="ACHKTW_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HFO6463904.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(11287..12180)
                     /gene="nanA"
                     /locus_tag="ACHKTW_002915"
     CDS             complement(11287..12180)
                     /gene="nanA"
                     /locus_tag="ACHKTW_002915"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008747 - N-acetylneuraminate lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HFO6463905.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(12302..13093)
                     /gene="nanR"
                     /locus_tag="ACHKTW_002916"
     CDS             complement(12302..13093)
                     /gene="nanR"
                     /locus_tag="ACHKTW_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417693.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HFO6463906.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVTDQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
     gene            13473..14840
                     /gene="dcuC"
                     /locus_tag="ACHKTW_002917"
     CDS             13473..14840
                     /gene="dcuC"
                     /locus_tag="ACHKTW_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HFO6463907.1"
                     /translation="MFGIIISVIVLITMGYLILKNYKPQVVLAAAGIFLMMCGVWLGF
                     GGVLDPAKSSGYLIVDIYNEILRMLSNRIAGLGLSIMAVGGYARYMERTGASRAMVSL
                     LSRPLKLIHSPYIILSATYVIGQIMAQFITSASGLGMLLMVTLFPTLVSLGVSRLSAV
                     AVIATTMSIEWGILETNSIFAAQVAGMKIATYFFHYQLPVASCVIISVAISHFFVQRA
                     FDKKDKNINHEQAELKALDNVPPLYYAILPVMPLILMLGSLFLAHIGLMQSELHLVVV
                     MLLSLTVTMFVEFFRKHNLRETMDDVQAFFDGMGTQFANVVTLVVAGEIFAKGLTTIG
                     TVDAVIRGAEHSGLGGIGVMIIMALVIAICAIVMGSGNAPFMSFASLIPNIAAGLHVP
                     AVVMIMPMHFATTLARAVSPITAVVVVTSGIAGVSPFAVVKRTAIPMAVGFVVNMIAT
                     ITLFY"
     gene            complement(14883..15380)
                     /gene="sspB"
                     /locus_tag="ACHKTW_002918"
     CDS             complement(14883..15380)
                     /gene="sspB"
                     /locus_tag="ACHKTW_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HFO6463908.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(15386..16024)
                     /gene="sspA"
                     /locus_tag="ACHKTW_002919"
     CDS             complement(15386..16024)
                     /gene="sspA"
                     /locus_tag="ACHKTW_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein SspA"
                     /protein_id="HFO6463909.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            complement(16419..16811)
                     /gene="rpsI"
                     /locus_tag="ACHKTW_002920"
     CDS             complement(16419..16811)
                     /gene="rpsI"
                     /locus_tag="ACHKTW_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFO6463910.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            complement(16827..17255)
                     /gene="rplM"
                     /locus_tag="ACHKTW_002921"
     CDS             complement(16827..17255)
                     /gene="rplM"
                     /locus_tag="ACHKTW_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFO6463911.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            complement(17474..18601)
                     /gene="zapE"
                     /locus_tag="ACHKTW_002922"
     CDS             complement(17474..18601)
                     /gene="zapE"
                     /locus_tag="ACHKTW_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HFO6463912.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSRP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFARGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLNDETRAQMDKLWLALAGAKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGDRLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            18795..19193
                     /gene="zapG"
                     /locus_tag="ACHKTW_002923"
     CDS             18795..19193
                     /gene="zapG"
                     /locus_tag="ACHKTW_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Z-ring associated protein ZapG"
                     /protein_id="HFO6463913.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQMPRDYSEGASGLLRTGAKRD"
     gene            19347..20714
                     /gene="degQ"
                     /locus_tag="ACHKTW_002924"
     CDS             19347..20714
                     /gene="degQ"
                     /locus_tag="ACHKTW_002924"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
                     /protein_id="HFO6463914.1"
                     /translation="MKKQTQLLSALALSVGLTLSASFQAVASIPGQVADQAPLPSLAP
                     MLEKVLPAVVSVRVEGTASQGQKIPEEFKKFFGDDLPDQPAQPFEGLGSGVIINASKG
                     YVLTNNHVINQAQKISIQLNDGREFDAKLIGSDDQSDIALLQIQNPSKLTQIAIADSD
                     KLRVGDFAVAVGNPFGLGQTATSGIVSALGRSGLNLEGLENFIQTDASINRGNSGGAL
                     LNLNGELIGINTAILAPGGGSVGIGFAIPSNMARTLAQQLIDFGEIKRGLLGIKGTEM
                     SADIAKAFNLDVQRGAFVSEVLPGSGSAKAGVKAGDIITSLNGKPLNSFAELRSRIAT
                     TEPGTKVKLGLLRNGKPLEVEVTLDTSTSSSASAEMITPALEGATLSDGQLKDGGKGI
                     KIDEVVKGSPAAQAGLQKDDVIIGVNRDRVNSIAEMRKVLAAKPAIIALQIVRGNESI
                     YLLMR"
     gene            20804..21871
                     /gene="degS"
                     /locus_tag="ACHKTW_002925"
     CDS             20804..21871
                     /gene="degS"
                     /locus_tag="ACHKTW_002925"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HFO6463915.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQGGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(21934..22872)
                     /gene="mdh"
                     /locus_tag="ACHKTW_002926"
     CDS             complement(21934..22872)
                     /gene="mdh"
                     /locus_tag="ACHKTW_002926"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030060 - L-malate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HFO6463916.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKTCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQSVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQSALEGMLDTLKKDIALGEEFVNK"
     gene            23307..23777
                     /gene="argR"
                     /locus_tag="ACHKTW_002927"
     CDS             23307..23777
                     /gene="argR"
                     /locus_tag="ACHKTW_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HFO6463917.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
BASE COUNT         5813 a         6341 c         6195 g         5585 t
ORIGIN      
        1 ctaacgactt cccttttagc cttaaagata aaatccattt taatttcagt aatttaataa
       61 agaattttgc gctaaagcac atttctgtac caataagctt gccatttgac ctgtatcagc
      121 tttcccgata agttggaaat ccgctggaag ctttctggat gagcagcctg ctcatcatat
      181 ttatgcagta attgagatcc cctcttcacc gtattaaccg atgcgaaaag gacaacaagg
      241 gggcgaatgc gaggcgcgcg tatgacacgc aaaccccgtc gccacgctct ttctgtgccc
      301 gtgcgcagcg gttcggaagt ggggttcccg cagagcctgg gggaggttca cgatatgttg
      361 tacgataaat cccttgagag ggataactgt ggtttcggcc tgatcgccca catagaaggc
      421 gaacctagcc acaaggtagt gcgtactgca atacacgcac tggcccgcat gcagcaccgt
      481 ggcgcgattc tcgccgatgg taaaaccggc gacggttgcg gcttgctgtt acaaaaaccg
      541 gatcgctttt ttcgcatcgt tgcgcaggag cgcggctggc gtttagcaaa aaactacgct
      601 gtcgggatgc tcttcctgaa taaagatcct gaactcgccg ctgccgcacg ccgcatcgtt
      661 gaagaagagc tgcaacgcga aaccttgtcg attgtgggct ggcgtgatgt ccccactaac
      721 gaaggcgtgc tgggtgaaat cgccctctcc tctctgcccc acattgagca aattttcgtt
      781 aacgctccgg caggctggcg tccgcgcgat atggagcgcc gtctgtttat cgcccgccgc
      841 cgcattgaaa agcgtctcga agccgacaaa gacttctacg tctgtagcct gtcgaatctg
      901 gtgaacatct ataaaggtct gtgtatgccg gcggatctgc cgcgctttta tctggatctt
      961 gcggacctgc gtctggaatc ggccatctgc ctgttccacc agcgcttctc cactaacacc
     1021 gtaccgcgct ggccgctggc gcagccgttc cgctacctgg cgcataacgg tgaaatcaac
     1081 accatcaccg gtaaccgcca atgggcgcgt gcgcgtacct ataaattcca gacgccgctg
     1141 atccctgatc tgcacgacgc cgcaccgttc gtcaacgaaa ccggttctga ctccagttcg
     1201 atggataaca tgctggaact gctgctggca ggcgggatgg atatcatccg cgccatgcgt
     1261 ctattagtac cacccgcctg gcagaacaac ccggatatgg acccggaact gcgtgccttc
     1321 tttgacttta actccatgca tatggagccg tgggatggcc cggcgggcat cgtgatgtcc
     1381 gatggtcgct tcgctgcctg taacctcgac cgtaacggtc tgcgtccggc acgctacgtc
     1441 attaccaaag acaagctcat cacctgcgcc tctgaagtcg gtatctggga ttatcagcct
     1501 gacgaagtgg tcgaaaaagg ccgcgtcggg ccaggcgaac tgatggtgat cgacacccgc
     1561 agtgggcgta ttctgcactc ggcagaaacc gatgacgatc tgaaaagccg ccatccatat
     1621 aaagagtgga tggagaaaaa cgtccgccga ctggtaccgt ttgaagatct gcccgatgaa
     1681 gaagtgggta gccgcgaact ggacgacgac acgcttgcca gctaccagaa acagtttaac
     1741 tacagcgcgg aagagctgga ctccgtaatt cgcgtactgg gcgaaaacgg tcaggaagcg
     1801 gtcggttcga tgggcgatga taccccattc gccgtgctct ccagtcagcc gcgcattatt
     1861 tacgactact tccgccagca gtttgcccag gtgactaacc cgccaatcga cccgctgcgt
     1921 gaagcgcatg ttatgtcgct cgccaccagt atcggtcgtg aaatgaacgt cttttgcgaa
     1981 gcagagggcc aggcgcaccg tttaagcttt aaatcgccga ttctgctcta ctccgatttc
     2041 aaacagctca cgacgatgaa agaggagcac taccgcgcag atacgctgga tatcaccttt
     2101 gacgtcacta aaaccacgct cgaagcgaca gtcaaagagc tgtgcgacaa agccgaaaaa
     2161 atggtacgta gcggcaccgt gctgctggtg ctctccgacc ggaatatcgc taaagatcgc
     2221 ctgccggttc cagccccgat ggcggttggc gcgatccaga cccgtctggt cgatcaaagc
     2281 ctgcgttgcg atgccaacat catcgtcgaa accgccagcg cccgcgatcc gcaccacttc
     2341 gccgtgttgc tgggcttcgg cgcgacggct atttatccat accttgccta tgaaacgctg
     2401 ggccgcctgg tagacaccca tgcgattgcc aaagattatc gtaccgtgat gctcaactac
     2461 cgtaacggca tcaacaaagg cttgtacaaa atcatgtcca aaatgggcat ctccaccatc
     2521 gcctcttacc gctgctcgaa actgtttgaa gcggtcggtc tgcacgatga tgtagtgggc
     2581 ctgtgcttcc agggggcggt cagccgcatt ggtggggcga gctttgaaga cttccagcag
     2641 gatctgctga acctgtcgaa acgtgcctgg ctggcgcgta agcccatcag ccagggcggc
     2701 ctgctgaaat acgtccacgg cggcgaatac cacgcttaca acccggacgt ggtgcgcacg
     2761 ctgcaacagg cggtacaaag cggcgagtac agcgactatc aggaatacgc gaagctggtt
     2821 aatgagcgtc cggcaaccac gctgcgcgat ctgctggcaa ttacgccggg tgaaaacgcg
     2881 gtcaacattg ctgatgttga accggcaagc gaactgttta aacgctttga taccgccgcg
     2941 atgtctatcg gcgcgttaag cccggaagcc cacgaggcgc tggcggaagc gatgaatagc
     3001 atcggcggta actcgaactc cggtgaaggc ggcgaagacc cggcgcgcta tggcactaac
     3061 aaagtgtcgc gcatcaagca ggtggcttct ggtcgcttcg gcgttactcc ggcgtatctg
     3121 gtcaatgccg acgtcattca gattaaagtc gcccagggcg cgaagccggg cgaaggcggt
     3181 cagttgccgg gcgacaaagt cactccttac atcgccaaac tgcgctattc ggtgcccgga
     3241 gtgacgctga tctccccgcc gccgcaccac gatatctact ctatcgagga cttagcgcag
     3301 ctcattttcg acctcaagca ggttaacccg aaagcgatga tctccgtgaa gctggtttcc
     3361 gaaccgggcg taggcaccat cgcgactggc gtggcaaaag cttatgccga tttaatcacc
     3421 atcgcaggct atgacggtgg caccggcgca agcccgctgt cgtcagtgaa atacgcaggt
     3481 tgcccgtggg agctggggct tgttgaaacc cagcaggcgc tggttgccaa tggtctgcgt
     3541 cataagatcc gtttgcaggt cgatggcggc ctgaaaaccg gtgttgatat catcaaagcg
     3601 gcgattctcg gcgcagaaag cttcggcttc ggcactggcc cgatggtggc gctcggctgt
     3661 aaatatctac gtatttgcca tctgaacaac tgcgcaacgg gtgtagcaac tcaggatgac
     3721 aaactgcgta agaaccacta tcacggcctg ccgttcaagg ttaccaatta ctttgaattt
     3781 attgcccgtg aaacccgcga gctgatggca cagctgggcg taacgcgtct ggtggatctg
     3841 attggtcgca ccgacctgct gaaagaactg gacggtttca ccgccaaaca gcaaaaactg
     3901 gcgctgtcga agctgctgga gaccgccgaa ccgcatccag gtaaggcact ctactgcacc
     3961 gaaaacaacc caccgtttga taacggcctg ctgaacgcgc agttgctgca acaggcgaaa
     4021 ccgtttgtcg atgaacgcca gagcaaaacc ttctggttcg atattcgcaa caccgaccgt
     4081 tctgtcggcg cgtcgctttc aggctatatc gcccagacgc acggcgatca ggggctggca
     4141 gccgatccta tcaaagcgta cttcaacggc accgcaggcc agagtttcgg tgtgtggaac
     4201 gcgggcggcg tggaactgta cctgaccggg gatgccaacg actatgtcgg taaaggcatg
     4261 gcgggcggct taatcgccat tcgtcctccg gttggttccg ccttccgcag ccatgaagca
     4321 agtattatcg gcaacacctg cctgtatggc gcgaccggtg gtcgtctgta tgccgcaggc
     4381 cgcgcgggtg aacgtttcgg cgtgcggaac tccggtgcta tcaccgtggt agaaggcatt
     4441 ggcgacaacg gctgtgaata tatgacgggc ggtattgtct gtattctggg taaaaccggc
     4501 gttaacttcg gtgcgggcat gaccggcggc ttcgcttacg ttctcgatga aagcggtgat
     4561 ttccgcaaac gcgttaaccc ggaactggtc gaggtcttaa gcgttgacga tctggcgatc
     4621 catgaagagc atctgcgcgg tcttatcacc gagcatgttc agcatactgg ctctcagcgc
     4681 ggtgaagaga ttctggcgaa ctggtcaacc ttcgccacta aatttgcgct ggttaaaccg
     4741 aagtccagtg atgtaaaagc actgctgggt catcgtagtc gtagcgcagc agagttgcgc
     4801 gtgcaggcgc agtaaggggt agcaacaatg agtcagaatg tttatcaatt tatcgacctg
     4861 cagcgcgttg atccgccaaa gaagccgctg aagatccgca aaattgagtt tgttgaaatt
     4921 tacgagccgt tttccgaagg ccaggccaaa gcgcaggctg accgctgcct gtcgtgcggc
     4981 aacccatact gcgagtggaa atgtccggta cacaactaca tcccgaactg gctgaagctc
     5041 gccaacgagg ggcgtatttt tgaagcggcg gaactgtcgc accagaccaa caccctgccg
     5101 gaagtttgcg gacgagtctg cccgcaagac cgtctgtgcg aaggttcctg cactctgaac
     5161 gatgagtttg gcgcggtgac catcggcaac attgagcgtt atatcaatga taaagcgttc
     5221 gaaatgggct ggcgtccgga tatgtccggc gtgaagcaaa ccggtaaaaa agtggcgatt
     5281 atcggcgcag gcccggcagg tctggcgtgt gcggatgtcc tgacgcgtaa cggcgtaaaa
     5341 gccgttgtct tcgaccgtca tccagaaatt ggcgggctgc tgaccttcgg tattccggcc
     5401 ttcaagctgg aaaaagaggt aatgacgcgt cgccgtgaaa tcttcaccgg catgggtatt
     5461 gaattcaaac tcaataccga agtgggccgc gacgtgcagc tggacgatct gctgagtgat
     5521 tacgatgccg tcttccttgg cgtcgggact tatcagtcaa tgcgcggcgg gctggaaaac
     5581 gaagacgccg atggcgtgta cgcagcgctg ccgttcctta tcgccaacac caaacagtta
     5641 atgggctttg gtgaaacccg cgacgaaccg ttcgtcagca tggaaggcaa acgcgtggtg
     5701 gtccttggcg gtggcgacac tgcgatggac tgcgtgcgta cgtccgtgcg ccagggagcg
     5761 aagcacgtta cctgtgccta tcgtcgtgat gaagagaaca tgccgggttc ccgccgcgaa
     5821 gtgaaaaacg cgcgggaaga aggcgtagag ttcaaattca acgtccagcc actggggatt
     5881 gaagtgaacg gtaacggtaa agtcagcggc gtaaaaatgg tacgtaccga aatgggcgaa
     5941 ccggatgcca aaggccgtcg ccgcgcggag atcgttgcag gttccgaaca tatcgttccg
     6001 gcagatgcag tgatcatggc gtttggtttc cgtccacaca acatggaatg gctggcaaaa
     6061 cacagcgtcg agctggattc acaaggccgc atcatcgccc cggaaggcag cgacaacgcc
     6121 ttccagacca gcaacccgaa aatctttgct ggcggcgata tcgtccgtgg ttccgatctg
     6181 gtggtaaccg ctattgccga aggtcgtaag gcggcagacg gtattatgaa ctggctggaa
     6241 gtttaagctc tgtcccagcg ccgcctcctc agaaaagagt cggcgctgaa attctccttt
     6301 ctttctggaa agcgcctcgc ataatctgaa gaaaaccaat gtaatacgct catctggatt
     6361 gttaccggta gatgcccggc gtatagctga ttccattatt caacgcagcg ccatataaag
     6421 ggacagataa tggaatcgct ctcagagaga acctcaacag gctaccagca aatccacgac
     6481 ggtattattc atctggtcga tagcgcccgg acggaaacgg tacgtagcgt taacgcgctg
     6541 atgaccgcga catactggga aattggccga cgaattgtcg aatttgaaca aggtggcgag
     6601 gccagggctg cgtatggtgc gcagctaatc aagcgactat caaaggattt aagtctaagg
     6661 tataagcgtg ggttctctgc aaaaaactta cgccaaatga ggctttttta cctctttttt
     6721 caacatgttg aaattcgcca gacagtgtct ggcgaattaa caccattggg aattccgcag
     6781 acaccatctg cagaattccc atcggcaaag atttggcaga cgctgtctgc caaatctttc
     6841 cccctgccct ggtccaccta cgtccgttta ctttccgtta aaaacgctga cgcccgcagc
     6901 ttttatgaaa aagagacgct ccgctgtggc tggtctgttc gccagctaga acggcaaatt
     6961 gcgacccaat tttatgagcg aacactactg tcacatgaca aatcagccat gctgcaacaa
     7021 cacgctccta ccgagacgca tattcttccg caacaggcga tacgcgatcc ctttgtgctc
     7081 gaatttctgg aattgaaaga tgaatattca gaatctgatt tcgaggaggc gctgatcaac
     7141 cacctgatgg atttcatgct ggaacttggg gatgattttg cctttgttgg tcggcagcga
     7201 aggttacgca ttgatgacaa ctggtttcgt gtcgatctgc tgtttttcca ccgccgttta
     7261 cgctgcctac taatcgtcga tctaaaagtg ggcaaattca gctatagcga tgccggacag
     7321 atgaatatgt atctcaacta cgccaaagag cactggacgc taccggatga aaatccgccc
     7381 atcggtctgg ttctctgtgc agagaaagga gccggagaag cacattatgc tctggcaggt
     7441 ttgcctaaca ccgttctggc aagcgaatat aagatgcaac tacctgatga gaaacgactc
     7501 gcagatgaac tcgttcgaac acaggcggtg ctagaggaag gctatagacg ccgttaattt
     7561 cagcggtttc gaattctgca gacagtgtct gcagaatctt taaaacgaac taagttttaa
     7621 gccattaaat cagccttaac cttcaccaca acctttttaa tctctccagg ctcgccgaca
     7681 acgcatcctg gtttatgcgg ttcacctggc ataaacacgg cgaacattcc cggttttaag
     7741 atgatggctt gctcgttatc aatggtgctg caaagctgat aatcatcctc atggtggaac
     7801 tcttcacact ggcgcgcagt gcctgccatg ccaaacagaa tccgttcctc accgtttaat
     7861 aacagttgga tatcaatgta ttgctcgtgc aattccgctt ttttctcgac gggcgattga
     7921 gtgttaaacg tcatgacatt cataaagata ttgtcgccct gtaattcgta acgacccggc
     7981 gctttttctt gcggtctggc agctaatgcc agcgttaacg cgtcctgtaa cgcaggatgt
     8041 aacccagcag acggtaatga ctgtacttca cccatcatca taatttttct ccctgggcca
     8101 acagcgcagc cccaagtaaa cctgcatcgt ggcggtagtg cgccgccagt aaatcaacat
     8161 gaaatgccgc tggctcctgc gccagatacg tttccaccag cgccagatac ccttctgcca
     8221 gcccaacgct gccaccgacc accacgcact ggcaatcagt tgtggcttta atatcagcga
     8281 tcagccttgc aagcgtacgt gcggagcggt gaatcagctg ctgcgcctgc tcgtcgccct
     8341 gcccggcgcg tgtgaaaata gttctcgcat ccgcgcccgc cagttcaccc tgcgctgccg
     8401 ccgcaatgcc gcgaccagaa gcaatcgcct ccacgcaacc tgtgcgtcca cagccacaga
     8461 ccgggccgtg tggatcggca agcgtatgcc cgatgtgccc cgccagaccg ccagggccgg
     8521 taagcagttt gccgccgctc actacaccgc cgccgacgcc agtggaaacg gtgataaaga
     8581 ccatatcggt tatatcgcct tccagcgcct gatactctgc ccatgctgcg gcctgcgcgt
     8641 cgttaatggc aatggtcggc aaattggtaa gttgttccag cgttttgact aacggaaagt
     8701 gtagcaatcc accgagatta tgcggattca gcgccagcaa actgccgtcg cgaataattc
     8761 cggttgaagc gatggcaacc cgctgcgcat gagcttgcaa cggagagact aatgcggata
     8821 aggcatcacg caaggcttct ggtgtctggc tggctggcgt aggaagttca cgacgatcgc
     8881 ggatctgccc gtcagcgcca atcagcgcgg cggcaagttt agtaccgccg atatcaatcg
     8941 ccagcgtggt catagcaccg cctttttcat cgctgtgttg taccactgac aaatgtgttc
     9001 aagacgcgta atggcagaac cgaccgtcac cgcccacgcg ccgtggcgca tcgcatctgc
     9061 cgcctgagca ggcgtgttgt aacgcccttc ggcaatcacc ctacatccgg cgtcgctcaa
     9121 cgttttcacc agcgccagat ccggctcttc tggcgtttca ggcgtggtat agccagaaag
     9181 cgtagtgcca ataatttcgg ctcccagctt ttggcatgcc aggccgtctt ccggcgttga
     9241 gcagtcggtc atcgccagta aaccgtgatg gtgaatacgt gccagcagcg tttcaacagg
     9301 caccggacgc gggcggtcgg tgccgtcaat ggcgataatg tccgcgcccg cctgcgccag
     9361 cgcatcaaca tcttcaatat aggccgtgat gcgtaccgga gaatcctcca gatcgcgttt
     9421 cacaattcca ataatcggca cgctcaccac cgcacgcgtg gcttgcagat ttgccacacc
     9481 ttcaatgcga atggcaaccg cgcccgcctg ttctgccgct aatgccatgg cggcgacgat
     9541 ttcgggttta tcgagcgggc tgtccggaac cggctggcag gagacaatca ggccaccgtt
     9601 agcagcgatt ttttgatcca gttgtgcaag taacgacata catcttccct tagcgaaagg
     9661 cccggtacat agaccgggca acaggattaa cttttggttt tgactaaatc gtttttggcg
     9721 ctgccaaatg gcacggcacc gctaaatggt ttgccgtcga tagcgtcatg agtacgcaat
     9781 gcttccgggc gcagccaacg ctgaacgcgg gaaggcatat ccagaccaat cagcaggatc
     9841 accacaaatg tcagactgaa cgatagcgat gccagcgcag tacccagatc cagacgttga
     9901 gcgatcaacg cgccgatgat tggggccagt gcgccgccca atgcgccaac gttgtaggta
     9961 aagcccaggc ccgctgcacg ctggtcggta tcgaaataac cgccaatcag tttcggtaag
    10021 atcccggcga tcccttgtcc aagcatttgc tggaagaaca gtaacagacc gagcacccag
    10081 acgtttgcgc cgccaatcgc aaataccgga ataatcaaca gctgcgaggc caacaggcta
    10141 caaacgtacg ctttgcgggt tcccagccag tcaccgagga agccgccgac acagcacccc
    10201 accgcagccc caaagccgct aaagaacagc acattggcga cagtatgcgg gttataagcc
    10261 agatcggttt tcagatacgt tggcagcagc gcctgaatcg gccatgagta gaggaaagca
    10321 aacaacacga ccaccatcag cattacgccc gttggccagc gtttacccgt actttgcacc
    10381 ataaagctga taaagattgc ggcgcataac agcccaagaa cagcgacgat cgcggcattt
    10441 tgcaggttac cggcgaagca aaaccacagc gcagtagccg ccgccagtgt cattacgata
    10501 ttggcaatgc gatgttcacc acggtagaga atatctacca tcgtgcgtac tggtgcttta
    10561 cctgcatgtt tctctttcca gtcttccgct tccgggatgt ttttacgcag ccagagagca
    10621 aagatgatag gcaaaatgcc gataaagaac agcgcacgcc agccccagac cggaaccacc
    10681 aggctataga cctgagcggc aacgacggcc cccacagaga agcctgaaat cagaaaacca
    10741 ctggctttgt tacgcagatg ttttggccag ctttcaatga cataggtggc gctggaaccg
    10801 tattcacccg ccatccccat gccgatgacc agacgcgcga taaacatggt gatgtagcct
    10861 ggcgcaaagc cgcaggccag cgtcccggcc gagaagagaa cgatgctggt gaccattgcc
    10921 agacgacgcc cgtagcggtc acccatagcg ccgagcatca ggccgccgaa ccagcgagag
    10981 ataaaggctg cggagatcag acttgccgcc tgcaccgtcg tcagcccgaa ttcaccttgt
    11041 acttcggtga gtaccagggc gattaaaacg aaatcaaaac cgtcaagcag atatcccaac
    11101 caggcagcgg aaaatgcccg ccattgtgca cgattgagat ggcgatacca cgggatattc
    11161 tgggttgtag tactcattgt gagtctccca cggtgggcga tgcccacacg ctttggtatg
    11221 aaaattgtag ggtacagatg cgtttatttc ccctcacccg gtaggggcga gcgaggggaa
    11281 aacaactcac ccgcgctctt gcatcaactg ctgggccagc gccttcagtt ctggcagata
    11341 tttttcatct accggtccaa acggtttgcg gcacagcggc acagaaacga catccatata
    11401 atggaggaca gttttcaggc cgcggaatac gcccgttttg atcagtaaat caatgacttt
    11461 attgcattca gtttgcagtt tctgcgcggt ctggatatcg ccttctttca gcgccttaac
    11521 gatcccctga tagcgccagc ccatgatgtt gtaggtacta ccgataccac catcagcgcc
    11581 cgccagcaga ccagaggcga agatttcgtc gtaaccgtta tagagcacaa gatcaggatg
    11641 ttcacgacgg atctgctcca tctgatagag atcgccagaa gtctgtttca gcgcaccgac
    11701 gccaggcaac gtaaccaggg tgttgatctg atccagggtc agttttaccc cactcagggc
    11761 tggaatgttg tacaccacca tcggcaaacc atccgctgaa tcaataattg cccgatagtg
    11821 atcgcagtgt tcttcaaagc tgaaaggata gtagaacggc gtgacggcgg agacggcatc
    11881 gaagccataa cgtttagccg atgccgcaag ttgttggctt tcggcggtgc tgacgcaacc
    11941 gacgtgggcg atgagtttaa tcttaccttt cgcctcttcg gcgacgattt ccagtacctg
    12001 ttcacgctcg gaaaggcttt gtacaaaggc ctcgccggtc gaaccaccca cgtataaacc
    12061 gtcgatgccc tgctgaatat tgaactgaac caggcgacgc agactcgctt tatccagtgc
    12121 ttgttgttga tcaaaaggag tcaggagtgc agccattacg ccacgtaaat tcgttgccat
    12181 aaatacctct gaagtgatgc ttgtctgata aacgatatac ctttatacct gttataccag
    12241 atcaattaag caacacccca tacagaaagc ttataatgcg atctgcttca ctaaagtggc
    12301 attatttctt tttgttggtg gtctgaccga aagcgtgcca ggtagcagag acgctgttga
    12361 gatgcgattg caacgcacga tcggcttcgt caggatcatg acggcggatc gcatcaacga
    12421 tcgcaatatg ctgttgataa ctaacgttgt tatgttcgtg cagtgcctga tcggtaaccg
    12481 ttgggcgtgc ggcaataagc cagtcgagca gggcaacgtg gatcgccatg aagattgggt
    12541 taccggggat ctccgccagc acgcggtgga aatcaacgtc tgaacgaatg aatgccgcgt
    12601 tgttatccag cgactgactg ttaatttcca gtgcttttgc cagcaaatcg atttgctcat
    12661 cggtggcatg ttcagccgca tagcgcacca ggctggattc aaagaacaga cgtaattgtt
    12721 cgaaatgggc aatcccacca ggatgagaaa ggaaatcttt cgccattccg gaaagctcgc
    12781 cgatgatggt gtccgcagaa ggacgtgaga cgcgggcacg ttcgccgttg ttgatttgca
    12841 ccagaccttt gcgttttaac gctgccagcg cttcgcgcac agaaggacgc ccgacgttaa
    12901 agaacgccat cagttcgcgt tcagacggta attgttcacc ttcgccaaat tcacgacggc
    12961 ggatcatctg ttccagctct tcttccacca tttcggagag ttttttacgc gccagcgggc
    13021 ggctacgcaa gttgcgacca attgcaggtg aagaatcttc ggtttgcgaa tcaaatgcgt
    13081 tcataaggcc cattctgtaa ggtcagtgtg attaacatca tcagtgacat cctatcacag
    13141 gattgaaagt aggggaaaat ggcaggtttt tctctttgtg cctcatcatt accataatta
    13201 acggaataat taactattgc gaaaaattaa tgtaacgcag ataaaaacat cccgtttgaa
    13261 ttatttataa gactattcac gagcattatg aatattatga atgtgttctt acaaaataat
    13321 cataagcgca tattttttaa tgaaaaatca cctcacctac aattaaaaac acgacatccg
    13381 caccataaat agacttgcaa aaaatataac atcattgttt tcaatctgcc gtttatggga
    13441 ttgaccgttt tcttttgaca cggagttcaa caatgttcgg cataattata tctgtcatcg
    13501 tattaattac gatgggctat ttgatcctga aaaactacaa acctcaggtg gtgctggctg
    13561 ccgcaggtat cttcctgatg atgtgcggtg tctggttagg gttcggtggt gtactcgatc
    13621 ccgccaaaag cagcggctac ttgatcgtcg atatttataa tgaaatcctg cgcatgctgt
    13681 ccaaccgcat tgccggattg gggctgtcga ttatggcggt gggcggttat gcccgctaca
    13741 tggagcgcac aggagccagt cgcgcgatgg tgagcttgct aagccgcccg ttaaaactca
    13801 ttcactcgcc gtatattatt ctgtcggcaa cttacgtcat cggccaaatc atggcgcagt
    13861 ttattaccag cgcctccggc ctgggtatgt tgctgatggt caccttattt ccgacgctgg
    13921 tgagtctggg agtaagtcgc ctctctgcgg tggcggttat cgcaaccacg atgtccattg
    13981 agtgggggat tctggaaacg aactccattt ttgcagccca ggtcgcggga atgaaaattg
    14041 ccacttactt cttccactac cagcttccgg tcgcctcttg cgtcattatc tcggtggcga
    14101 tctcccactt tttcgtgcaa cgcgcttttg acaaaaaaga taaaaatatc aatcacgaac
    14161 aggcagagct aaaagctctc gataatgtcc cgccgctcta ttacgccatt ttacctgtga
    14221 tgccgttaat cttgatgctc ggctcgctgt tcctcgccca catcgggctg atgcagtcag
    14281 aactgcatct ggtggtggtg atgttactga gtttgacggt gacgatgttt gttgagttct
    14341 tccgcaagca taacttgcgc gaaacaatgg acgatgtgca ggcgtttttt gacggcatgg
    14401 gtacgcagtt tgccaacgtg gtaacgctgg tggtcgcggg tgaaatattt gcgaaaggct
    14461 taacgacgat tggcactgtt gatgcggtta tcaggggtgc tgagcattct ggtctgggcg
    14521 gtattggcgt gatgattatt atggcgctag tcattgccat ttgtgccatt gtgatgggct
    14581 ctggcaatgc gccgtttatg tcatttgcca gtcttattcc gaatatcgca gccggactac
    14641 atgtaccagc ggttgtaatg attatgccga tgcattttgc cacgacgcta gcgcgcgcgg
    14701 tttcgccgat tactgcggtg gtggtcgtta cgtcaggaat tgcaggcgtt tcgccttttg
    14761 cggtggtgaa gcgaacagcg atccccatgg cagtcggttt cgtggtgaat atgattgcca
    14821 caatcacgct attttattaa gccattaaaa gacaaaacag gccaccaggg cctgtttttg
    14881 tattacttca caacgcgtaa tgccggtcga ccaccgcgtg gtggctgcgg aggttcatcg
    14941 tcaggatgag tgtcatcatc gtgatctggc ttgtcgccat caataaccga cataacggtt
    15001 tcgttgtctg ccgatgcctc ttcatcattc atgatgctgg tatcttcatc gtaggcagct
    15061 tcaggctcaa acatcgtgcc tgcgccattt tcacgggcgt agatagccag cacggcagcc
    15121 agcggcacag aaacctgacg cggaatgccg ccaaagcgcg cgttaaagcg tacctcatca
    15181 ttcgccagtt ccagattgcc gacagcacgc ggcgcaatgt tgagtacgat ttgcccgtca
    15241 cgcgcatatt ccataggaac ctgcacgcca gggagcgtca catccaccac caagtgcggc
    15301 gtgagctggt tatccagcaa ccactcatag aatgcacgca gcagataggg acgacgtggt
    15361 gttagctgtg acaaatccat acagattaac tccggcccag acgcatttca cgttctgctt
    15421 cagttaaaga agcaaggaaa gagtcacgct caaagacgcg ggtcatatag cctttcagct
    15481 ctttcgcacc cgggccgctg aactcgatgc ccagttgcgg cagacgccac agcagcggag
    15541 caagatagca atcgaccagg ctgaactcat cgctcaggaa gtacggcttc tgaccgaaga
    15601 ccggcgcaat cgccagcagt tcttcgcgca gttgcttacg tgcggcatct gcttcagaag
    15661 ctgaaccgtt gatgatggtg ttcatcagcg tgtaccagtc tttttcgatg cgatgcatgt
    15721 acagacggct ttcaccgcga gctaccgggt aaacaggcat cagtggcgga tgcgggaaac
    15781 gctcatccag atattccata atgatgcgag attcccacag ggtcagctca cgatccacca
    15841 gggtcggaac gctctgattc gggttgaggt caatcagatc ctgaggcgga ttgtcctttt
    15901 ccacgtgttc gatctcgaaa cttacacctt tctcagccag cacaatgcgg acctgatggc
    15961 tatagatgtc agtaggaccg gaaaacagcg tcattaccga acgtttgttg gcagcgacag
    16021 ccatgaaaac ctccaggtat agtcagaatt tttactgcta ccagccacca ggtggccagt
    16081 cagaagttgt gttacccagt aaggaacgac tctctttgtt cgaaaatcaa acaaaaaatg
    16141 agcaataccc gacatttggg cagaaaattg gatgatagtt taccagattt tgcgaccttt
    16201 gtggtgagtc gatgccggaa atggggaaaa agagatgcgc tttagtctga aatagttgac
    16261 ttagtccctt attggcgatg tggtttttgt tttgcctgtc tgtcagacgg tagcaaaaag
    16321 caactttcca atttttacgc tgattcagaa tttaaccata aaaaaacccg ccgaagcggg
    16381 ttttttcgca aattgttttc tgccggagca gaagccaatt aacgtttgga gaactgcgga
    16441 cgacgacgtg ctttacgcag accgactttc ttacgttcaa cctgacgagc gtcacgagta
    16501 acgaagccag ctttacgcag ttcagaacgc agggactcgt cgtattccat cagagcgcgg
    16561 gtgataccgt gacggatcgc accagcctga ccagagatac caccaccttt aacggtgatg
    16621 tacaggtcca gtttctcaac catgtcgacc agttccagcg gctgacgaac taccatgcga
    16681 gcagtttcac gaccgaagta ctgttccaga gaacgttggt tgattacgat tttaccgttg
    16741 cccggtttga tgaaaacgcg agctgcggaa cttttgcggc gaccagtgcc gtagtattga
    16801 ttttcagcca ttgcctataa tcccgattag atgtcaagaa cttgcggttg ctgtgccgcg
    16861 tggttgtgct cgttacccgc gtaaactttc agtttacgga acatagcacg acccagcggg
    16921 ccttttggca acatgccttt aaccgcgatt tcaatcacac gctcaggacg gcgagcaatc
    16981 atctcttcaa aggtcgcttg tttgatacca ccgatgtggc cggtgtggtg atagtacact
    17041 ttgtcagtac gcttgttgcc ggttacagca actttgtcag cgttcagaac gatgatgtaa
    17101 tcaccggtat ctacgtgcgg agtgtattcc gctttgtgct taccgcgcag gcgacgagcc
    17161 agttcagtag ccagacggcc cagagtttta ccggtcgcgt caacaacata ccagtcgcgt
    17221 tttacggttt ctggtttagc tgtaaaagtt ttcattaaaa gcttacccaa taaatagtta
    17281 cacgttggtg aacacccaaa cgtcttcaat tgttgaggtt cacacgacaa agtccggcaa
    17341 acctacccct tcgaatagcc tatgccagca cacaaaaagt tttgggaaaa aaactttctt
    17401 gtaacgtggg gtcgcaggat tatagagaag tcggggtcaa agatcgaccc ctttttgtga
    17461 tttgtgacag gttttaaccc gccaaatgct cgcgcttcag atactcttcg ctttgcatct
    17521 cttgcagacg tgacaggcaa cgctggaact caaacttcag ccgatcgccc tgataaattt
    17581 catacagcgg cacttctgca ctcaccacta atttgacatg gcgctcgtaa aactcatcca
    17641 ccagcgcaat aaagcgccgc gcttcgctct ccatcaaccg cgtcataact ggtacatcaa
    17701 acaacatgac cgtatgaaag agacgtgaga gcgcaatata gtcatgctga ctgcgggcgt
    17761 cgacgcacag cgtagtaaaa gagaccgcca gcgtctggtt ctcgacgccc attgtcgcca
    17821 atggccgatg gttgatttct aacgtcggtg aattttctcg tttcgccccc gccagcgcca
    17881 accatagttt atccatctgc gcccgggttt catcgttgag tggcgaaagc cacagatgcg
    17941 cctgagtgag tgtacgcagt cgataatcaa caccagcgtc cacgttcatt acatcacaat
    18001 gctgtttaat ggcatcgatt gcaggcagaa aacgcgcacg ttgcaggcca tttcgataaa
    18061 gttcatccgg cggaatattt gacgtcgcta ccagggtaat gccgcgggcg aacagggctt
    18121 tcatcagacc gccaagtagc atggcgtcgg taatatcaga aacaaaaaat tcgtcaaaac
    18181 agagcacgtc agtttcggct ttaaagcgat cggcaataat ttccagcgga tcggtctgcc
    18241 cctgtaaggc agttagctct tcgtgcaccc gcagcataaa acggtgaaag tgcaggcgct
    18301 gtttccgctc tcccggcagg ctttgataga aaaggtccat cagccaggtt ttcccgcgtc
    18361 ctacaccgcc ccacatatat aagccacgca ctggcgtatg ctttgtgtct tcgcgtttac
    18421 cccacagctt accgacccgc gccattagcc cactcgtcct gggggctggt ggcctgctat
    18481 tgatgagttc ttgataaata atttccaggc ggctgacggc ctctttttga acgtcgtcgg
    18541 gttgatggct gccttcattg agcgccttca ggtattgcga tgttggggta acgctttgca
    18601 tgatcttatt gttattcctt gaataatcgg tgcgccgttg ttcacggttg acgaaaaaaa
    18661 ggccgttcta cactacgcga tatgcagtcg ggattccact tctgtggaat taacggttat
    18721 agtggcataa tcagccgcag gcatggagcc tgaagccaac accctacgga aacaaaagac
    18781 aacgggagat gttcatgacc tgggaatatg cgctaattgg gttagtcgtc ggcatcatta
    18841 ttggtgctgt ggccatgcgt tttggtaatc gtaaactacg ccagcaacag gcgttgcagt
    18901 acgaactgga aaagaataaa gctgaactgg acgagtatcg cgaagagctg gttagccact
    18961 ttgcccgcag cgcggaatta ctggatacca tggcgcacga ctatcgccag ctgtatcagc
    19021 acatggcgaa aagctccagc agcctgctgc cggaactgtc tgctgaagca aacccgttcc
    19081 gtaatcgtct ggcagagtct gaagccagca acgatcaggc accggtgcag atgcctcgcg
    19141 actattctga aggcgcatcc ggcctgctgc gtactggcgc gaagcgcgac taatttattt
    19201 ttcgggcgca gccattgcgc cctcctcttc tctccctccc cgactatcat ttaatctggt
    19261 gtctcattgt tagccgtctg aaaattcaat aacatcaaac tgttttgaat ctcttttctt
    19321 atcattcagg tacgagagca ggaataatga aaaaacaaac ccagctgttg agtgcattag
    19381 cgttaagtgt cgggttaact ctctcggcgt catttcaggc cgtcgcgtcg attccaggcc
    19441 aggttgccga tcaggcccct ctccccagtc tggctccaat gctggaaaaa gtgcttccgg
    19501 cagtggtgag cgtacgggtg gaaggaacgg ccagtcaggg acagaaaatc ccggaagaat
    19561 tcaaaaagtt ttttggtgat gatttaccgg atcaacctgc acaacccttc gaaggtttag
    19621 gttccggtgt catcatcaac gccagtaaag gctatgtgct gaccaacaac catgtgatta
    19681 atcaggcaca gaaaatcagt attcagctca atgatgggcg cgagtttgat gcaaaactga
    19741 ttggtagcga tgaccagagc gatatcgccc tgttacaaat tcaaaacccg agcaaattaa
    19801 cgcaaatcgc tattgccgac tccgataaat tgcgcgtcgg tgattttgcc gtagcggtcg
    19861 gtaacccatt tggccttggg caaaccgcca cctctggcat tgtttccgca ttaggccgca
    19921 gcgggttgaa tcttgaaggt ctggaaaact ttatccagac agatgcttcc attaaccgcg
    19981 gtaactccgg cggtgcacta ttaaacctta acggtgagtt aattggcatc aacactgcaa
    20041 tccttgcgcc tggcggcggg agcgtcggga ttggatttgc catccccagt aatatggcgc
    20101 gaacactggc gcagcagctt atcgactttg gtgaaatcaa acgcggtttg ttaggcatca
    20161 aaggcaccga gatgagtgcc gatatcgcca aagccttcaa ccttgacgtg cagcgtggcg
    20221 cgtttgtcag cgaagtgttg ccaggttctg gctcggcaaa agcgggcgtc aaagcgggcg
    20281 atattattac cagcctcaac ggcaaaccgc tgaatagctt tgctgagttg cgctctcgta
    20341 tcgcgactac agagccgggc acgaaagtga agcttggcct gctgcgtaac ggcaaaccac
    20401 tggaagtaga agtgacgctt gataccagca cctcttcgtc ggccagcgct gaaatgatca
    20461 cgccagcgct ggaaggtgca acgttgagcg atggtcagct aaaagatggc ggcaaaggta
    20521 ttaagatcga tgaagttgtc aaaggaagcc cagctgctca ggctggcttg caaaaagacg
    20581 atgtgatcat tggcgtcaac cgcgatcggg tgaactcgat tgctgaaatg cgtaaagtgc
    20641 tggcggcaaa accggccatc atcgccctgc aaattgtacg cggtaatgaa agcatctatc
    20701 tgctgatgcg ttaatgtcgt aaaccgggca tcaggcttac gtgtgatgtc cggttaactc
    20761 gtggtatgct gctgccgttc ccttttttaa tgacgcctcc atcatgtttg tgaagctctt
    20821 acgttccgtt gcgattggat taattgtcgg cgctattctg ctggttgcca tgccttcgct
    20881 gcgcagcctt aacccgcttt ccactccgca atttgacagt accgatgaga cgcctgccag
    20941 ttataatctg gcggttcgcc gcgccgcgcc agcggtggtt aacgtttaca accgtggttt
    21001 gaacaccaac tctcacaacc agcttgagat ccgcaccctg ggatccggtg taatcatgga
    21061 tcaacgcggt tatatcatca ccaataaaca cgtcatcaac gacgccgatc agatcatcgt
    21121 cgccttacag gatggacgtg tatttgaagc attgctggtg ggatctgact ctctaaccga
    21181 tctggcggta cttaaaatta atgccactgg cggtttacct accattccga tcaatgcacg
    21241 tcgcgtaccg cacattggcg acgtggtact ggcgatcggt aacccgtaca acctcgggca
    21301 gaccattacc caggggatta ttagtgctac gggtcgaatc ggtctgaacc cgaccgggcg
    21361 gcaaaacttc ctgcaaaccg atgcttccat taaccacggt aactctggcg gcgcgctggt
    21421 gaactcgctg ggagaactga tgggcattaa cacgctgtcg tttgataaga gtaacgatgg
    21481 cgaaacgccg gaaggtatcg gctttgcgat tcctttccag ttagcaacca aaattatgga
    21541 taagctgatc cgcgatggtc gcgtgatccg cggctacatt ggtatcggtg gacgcgagat
    21601 cgcaccactg cacgcgcagg gcggtggtat agatcaactg caagggatcg tggttaatga
    21661 agtgtcacct gacggcccgg cggcgaatgc gggtattcag gtcaacgatc tgattatttc
    21721 ggtggataac aaaccggcca tctctgctct ggagacgatg gatcaggtgg cagaaattcg
    21781 ccctggttcg gtgatcccgg ttgtagtgat gcgtgatgat aagcagttaa cgctgcaggt
    21841 caccattcag gaatatccgg caaccaatta agtcgagtgc tcaaaacaaa aaaccggagt
    21901 ctgtgctccg gttttttatt attcgctaat taattactta ttaacgaact cttcgcccag
    21961 ggcgatatct ttcttcagcg tatccagcat accttccagt gcgctctgtt caaatgcgct
    22021 cagggtaccg atagatttac gctcttccac gccgttttta cccagcagca gcggttgaga
    22081 gaagaaacgg gcgtactgac cgtcgccttc aacgtaggca cattcgacaa cgctttgttc
    22141 gccctgcagt gcacgaacca gagacagacc aaaacgtgca gctgcctggc ccatagacag
    22201 ggttgcagac ccgccaccgg ctttcgcttc aaccacctca gtacccgcgt tctggatacg
    22261 tttggtcaga tcagccactt cctgctcggt aaaactaacg ccaggaacct gtgacagcag
    22321 cggcagaatg gtaacaccag agtgaccacc aataaccggc acttcaactt cgcctggctg
    22381 tttgcctttc agttccgcaa caaaagtatt ggaacgaatg atatccagcg tggtaacgcc
    22441 gaacagtttg tttttgtcat aaacaccggc ttttttcagc acttcagcag caatcgcaac
    22501 tgtggtgtta accgggttag tgataatacc aatgcacgct ttcgggcagg ttttcgcaac
    22561 ttgctgtacc aggtttttca cgatgccggc gttaacgtta aacaggtcgg aacgatccat
    22621 acccggttta cgcgctacac ctgcagagat aagaacgaca tctgcgcctt ccagcgccgg
    22681 agtcgcatct tcaccagaaa aacctttgat tttcacagca gtagggatat ggctcagatc
    22741 gacagccaca ccgggagtca ctggagcgat atcatacaga gagagttctg aacctgaagg
    22801 cagttgggtt tttaacagta gtgcaagcgc ctggccaata ccgccagcag cgccgaggac
    22861 tgcgactttc atcctaaact ccttattata ttgataaact aagatatgtt gctccgctgc
    22921 cgcgacctta atccacaaaa ttgccgttta caatgaccac atctcaagaa tgtgtagtca
    22981 cgcaagttta gcgtttatgc atttaattgc cgtaatcagg aacctaactt acgtaattaa
    23041 cagtcgctta tgcattagcg caacattcca acaggtggtg acaatatacc ctaccgttca
    23101 gccaaaacaa catcaatttg ataacaatta atttactttt aagcagaatt tgcatgccgt
    23161 gacgcaggca tgtttctcaa taacgaaatt tgataaaatc ccgctctttc ataacattat
    23221 ttcagccttc ttcagggctg actgtttgca taaaaattca tctgtatgca caataatgtt
    23281 gtatcaacca ccatatcggg tgacttatgc gaagctcggc taagcaagaa gaactagtta
    23341 aagcatttaa agcattactt aaagaagaga aatttagctc ccagggcgaa atcgtcgccg
    23401 cgttgcagga gcaaggcttt gacaatatta atcagtctaa agtctcgcgg atgttgacca
    23461 agtttggtgc tgtacgtaca cgcaatgcca aaatggaaat ggtttactgc ctgccagctg
    23521 aactgggtgt accaaccacc tccagtccat tgaaaaatct ggtgctggat atcgactaca
    23581 acgatgcagt tgtcgtgatt cataccagcc ctggcgcggc gcagttaatt gctcgcctgc
    23641 tggactcact gggcaaagca gaaggtattc tgggcaccat cgctggcgat gacaccatct
    23701 ttaccacccc tgctaacggt ttcaccgtca aagacctgta cgaagcgatt ttagagctgt
    23761 tcgaccagga gctttaatct ctgccccgtc gcttctggcg gcggggaaaa tgttgcttat
    23821 ccctctcaac cccctgcttt cccctgcgat taatttaacg aatagtgcgt tttactgcga
    23881 catgtcattc acacaatgaa tacataaggt aaaaaaagca cattatgcaa aatt
//
LOCUS       DAZFQD010000074        23677 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.074, whole genome shotgun sequence.
ACCESSION   DAZFQD010000074 DAZFQD010000000
VERSION     DAZFQD010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23677
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.074"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            44..568
                     /locus_tag="ACHKTW_002928"
     CDS             44..568
                     /locus_tag="ACHKTW_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000756357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbria major subunit LpfA"
                     /protein_id="HFO6463918.1"
                     /translation="MKKVLFALSALALTSTSVFAADAGDGTVKFTGEIVDAACVVSPD
                     TQKQEVVLGQVNKSVFTAMGDKSTATPFKIKLENCDISTFKNVEISFNGVGDADNSKL
                     VSVSTEPGAATGVGIGIYDNTNTLVDLNTGKSATVLKEGQTVLYFTANYVSTKDAVTT
                     GYGNAEVDFNLTYN"
     gene            652..1338
                     /gene="lpfB"
                     /locus_tag="ACHKTW_002929"
     CDS             652..1338
                     /gene="lpfB"
                     /locus_tag="ACHKTW_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000822474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial biogenesis chaperone LpfB"
                     /protein_id="HFO6463919.1"
                     /translation="MKRISFAVLALLMTAQQCFAGGVALSTTRVIYDGGKKEASLTVE
                     NHNKNEEFLIQSWIDDVNGNKKTPFIITPPLFKLDPDKNNVLRIVNIEDTLPKDRESV
                     YWINVKAIPAKSAENENKNVLQIAVRTRLKLFYRPKGLPGSNLEAMKQLRFQCQNNQI
                     VVNNDSAYNLTFNQFSINGQKIERAGMVLAKGKLNINLPAGTGNAHEVKYSIINDFGV
                     AGEMLTKPIN"
     gene            1362..3893
                     /gene="lpfC"
                     /locus_tag="ACHKTW_002930"
     CDS             1362..3893
                     /gene="lpfC"
                     /locus_tag="ACHKTW_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001759518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane usher protein LpfC"
                     /protein_id="HFO6463920.1"
                     /translation="MHWMHLPLYHYRAHFSFSLLALTIASSLPAYGGKFNPKFLENVQ
                     GIDQHIDLSVYDSPVGQQIPGKYRVSVFVNEEKMASRTLDFSTASEAKRKASGESLMP
                     CLSRVQLEEMGVRVDSFPALKMSPPEACVAFDEIIPQATSRFDFNTQTLHLSFPQAAM
                     MMTARGTVDPSRWDEGIPALLLDYSFSGSNGRNEGTGSSSDSTSDSYYLNLRSGLNVG
                     PWRLRNNSIWNRTDGKNQWDNVGTSLNRAIIPLKSQITLGDTATPGEIFDSVQMRGAL
                     LASDDEMLPDSQRGFAPVVRGIAKSNAEVSIEQNGYVIYRTFVQPGAFEINDLYATSG
                     SGDLTVIIKEADGSEQRFIQPFSAVAIFQREGYLKYSLAAGEYRAGNYDSDKPHFGQF
                     TAMYGLPWGMTAYGGALLSADYNALALGLGKNFGTIGAVSVDVTQAKSQLRNNEKEEG
                     QSYRFLYSKSFEGGTDLRLLGYKYSTSGYYTFQEATDVRSDADSDYRRYHKRSQIQGN
                     ITQQLGDYGSVYFNMTQQDYWNVDGKENSLSAGYHGHIGRVNYSVAYTWTRSPEWEED
                     DRLWSFSVSIPLGGAWSSYRMTTDQNGKTSQQASVSGTLLEDRNLSYNVQQGYTSNGV
                     GYSGSVNMGYMGGSGNIDVGYNYSKDNQQVNYGVRGGVIVHSEGITLSQPLGESLAIV
                     SAPGARGGHVVNSSGVEVDWMGNAVVPYLTPYRETIVELRSDTLGQNVELQEAFQKVV
                     PTRGAVVRSRFDTRVGYRVLMSLKQANGNAVPFGATAALIDESKPASSIVGEEGQLYI
                     SGMPEEGELQVSWGNEQAQRCRVPFRLPENKDNAAIVMVNAVCEK"
     gene            3904..4959
                     /gene="lpfD"
                     /locus_tag="ACHKTW_002931"
     CDS             3904..4959
                     /gene="lpfD"
                     /locus_tag="ACHKTW_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000694923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial protein LpfD"
                     /protein_id="HFO6463921.1"
                     /translation="MKIKNLLAGMVLLGTSTFAGNIWAADWGPCQTSNGVAHEYSFDF
                     VQTIQVPSENKAGKILTQPFALGTKYSAYCECPDPIPDNGVVTYFKGVTLLPEPGTVD
                     GYYKFNNYIDILTKIYVYTQLDIPVPFTDRSNGTAQKECTPYTANNWGTGGKGSISIY
                     ISHPFVGQMIIPKTRVASLFGTKKKGVYNDSQPMANVSISGSITVTQGCELAAGTVLD
                     IPFGEYQAHDFKGRAGQPPQNVQKVQKELSFDCNNISDGVKIYLSIDATPNTAYPSAI
                     DLGNADVGAVIEDGKGNILKPNDSNSLLEMNPGSLYEDVKRKATTTITAYPVSTTGKL
                     PAAGDYSGIATIHVELE"
     gene            4965..5489
                     /gene="lpfE"
                     /locus_tag="ACHKTW_002932"
     CDS             4965..5489
                     /gene="lpfE"
                     /locus_tag="ACHKTW_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial protein LpfE"
                     /protein_id="HFO6463922.1"
                     /translation="MKRTILALCLLPLSFSSFAATTDLGPKGTLKFTLKISQGACELV
                     KDSVEVDMGTAVLKKPVRIGTEINPTPFSIGLKNCSEVVRAYVTMDGTPNADDSNLFA
                     LDAGGATGVGLKIKTAAGVQQFPENTTPTPVEFAINGDGAHQLNYIASYVPIRADATI
                     GRADATVDFTVQYE"
     gene            5742..7433
                     /gene="eptB"
                     /locus_tag="ACHKTW_002933"
     CDS             5742..7433
                     /gene="eptB"
                     /locus_tag="ACHKTW_002933"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HFO6463923.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GVSAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LAVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPANAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQAKEAAAN"
     gene            7525..7601
                     /locus_tag="ACHKTW_002934"
     tRNA            7525..7601
                     /locus_tag="ACHKTW_002934"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7559..7561,aa:Pro,seq:cgg)
     gene            8346..9953
                     /gene="dppA"
                     /locus_tag="ACHKTW_002935"
     CDS             8346..9953
                     /gene="dppA"
                     /locus_tag="ACHKTW_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HFO6463924.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTRELNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQAPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            10104..11123
                     /gene="dppB"
                     /locus_tag="ACHKTW_002936"
     CDS             10104..11123
                     /gene="dppB"
                     /locus_tag="ACHKTW_002936"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HFO6463925.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHAQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRLQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            11133..12035
                     /gene="dppC"
                     /locus_tag="ACHKTW_002937"
     CDS             11133..12035
                     /gene="dppC"
                     /locus_tag="ACHKTW_002937"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HFO6463926.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVVIVLF
                     IAIFANWIAPYNPAEQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            12046..13029
                     /gene="dppD"
                     /locus_tag="ACHKTW_002938"
     CDS             12046..13029
                     /gene="dppD"
                     /locus_tag="ACHKTW_002938"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HFO6463927.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            13026..14030
                     /gene="dppF"
                     /locus_tag="ACHKTW_002939"
     CDS             13026..14030
                     /gene="dppF"
                     /locus_tag="ACHKTW_002939"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding subunit
                     DppF"
                     /protein_id="HFO6463928.1"
                     /translation="MSTQEATSQQPLLQAIDLKKHYPVKKGMFAPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIEMPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLTGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(14060..15331)
                     /gene="yhjV"
                     /locus_tag="ACHKTW_002940"
     CDS             complement(14060..15331)
                     /gene="yhjV"
                     /locus_tag="ACHKTW_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transport family protein"
                     /protein_id="HFO6463929.1"
                     /translation="MQHNTLSKHNQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPATWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLVAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRMLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
     gene            15807..15914
                     /locus_tag="ACHKTW_002941"
     CDS             15807..15914
                     /locus_tag="ACHKTW_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFO6463930.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            16290..16397
                     /locus_tag="ACHKTW_002942"
     CDS             16290..16397
                     /locus_tag="ACHKTW_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFO6463931.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            16773..16880
                     /locus_tag="ACHKTW_002943"
     CDS             16773..16880
                     /locus_tag="ACHKTW_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFO6463932.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            17256..17363
                     /locus_tag="ACHKTW_002944"
     CDS             17256..17363
                     /locus_tag="ACHKTW_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFO6463933.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            complement(17450..19129)
                     /gene="bcsG"
                     /locus_tag="ACHKTW_002945"
     CDS             complement(17450..19129)
                     /gene="bcsG"
                     /locus_tag="ACHKTW_002945"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HFO6463934.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPRYSLHRLRHWIALPIGFALFWHDTWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGAPVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIEFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(19126..19317)
                     /gene="bcsF"
                     /locus_tag="ACHKTW_002946"
     CDS             complement(19126..19317)
                     /gene="bcsF"
                     /locus_tag="ACHKTW_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0052324 - plant-type cell wall cellulose
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HFO6463935.1"
                     /translation="MMTISDIIEIIVVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(19314..20885)
                     /gene="bcsE"
                     /locus_tag="ACHKTW_002947"
     CDS             complement(19314..20885)
                     /gene="bcsE"
                     /locus_tag="ACHKTW_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis c-di-GMP-binding protein
                     BcsE"
                     /protein_id="HFO6463936.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPEKIKLFSMLNHEKGLYYLTRDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGIWTLVQSEEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
     gene            21158..21346
                     /gene="bcsR"
                     /locus_tag="ACHKTW_002948"
     CDS             21158..21346
                     /gene="bcsR"
                     /locus_tag="ACHKTW_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsR"
                     /protein_id="HFO6463937.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDIVALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            21358..22110
                     /gene="bcsQ"
                     /locus_tag="ACHKTW_002949"
     CDS             21358..22110
                     /gene="bcsQ"
                     /locus_tag="ACHKTW_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HFO6463938.1"
                     /translation="MAVLGLQGVRGGVGTTTITAALAWSLQMLGENVLVVDACPDNLL
                     RLSFNVDFTHRQGWARAMLDGQDWRDAGLRYTSQLDLLPFGQLSIEEQENPQHWQTRL
                     SDICSGLQQLKASGRYQWILIDLPRDASQITHQLLSLCDHSLAIVNVDANCHIRLHQQ
                     ALPDGAHILINDFRIGSQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAKQPVGEY
                     RSDALAAEEILTLANWCLLNYSGLKTPVGSAS"
     gene            22107..23624
                     /gene="bcsA"
                     /locus_tag="ACHKTW_002950"
                     /pseudo
     CDS             22107..23624
                     /gene="bcsA"
                     /locus_tag="ACHKTW_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417990.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016760 - cellulose synthase (UDP-forming)
                     activity [Evidence IEA];
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
BASE COUNT         5917 a         5783 c         6267 g         5710 t
ORIGIN      
        1 tgaacaacat caagcatatt tatctatata aatggagtta taaatgaaaa aggttctgtt
       61 tgccttatct gcgcttgcgc tgacctctac ttccgtattc gcagctgatg cgggcgacgg
      121 tactgttaaa ttcaccggtg agattgttga cgccgcttgt gttgtgtctc cagacactca
      181 aaaacaagaa gttgttctgg gtcaggtgaa caagtctgta tttactgcta tgggtgataa
      241 atccacagca acgccgttca aaatcaaact ggaaaactgc gatatctcca ctttcaaaaa
      301 tgtggaaatc agctttaacg gcgttggcga tgcagacaac agcaaactgg tttctgtaag
      361 cactgaacca ggtgccgcaa ctggcgtagg tatcggtatt tatgataaca ccaataccct
      421 ggttgacctg aatactggta aatctgctac tgtcctgaaa gaaggtcaga ccgttctgta
      481 tttcaccgct aactatgtct ctaccaaaga tgcagtaacc acgggttacg gtaacgctga
      541 agtcgacttc aacctgactt acaactaatc gatcctgacg gaacgatcat ctctcaatgg
      601 caacagagac tgtgttgcca ttattcacac caatgactca gggaccgaat catgaaacgt
      661 attagctttg ctgtgctggc acttttaatg accgcacaac agtgttttgc tggtggcgta
      721 gccttaagca cgacgcgtgt tatttatgat ggcggcaaga aagaagcgtc gctgaccgtt
      781 gaaaaccata ataaaaatga agagtttttg atccagtcat ggattgatga tgtgaatggc
      841 aataaaaaaa caccgtttat tattacaccg ccattattca aacttgatcc tgataaaaat
      901 aacgtcttac gtatcgtcaa tattgaagat accttaccta aagatcgtga atccgtttac
      961 tggattaatg taaaggccat tcctgcgaaa agtgctgaaa atgaaaataa aaacgtctta
     1021 cagatagccg ttcgcacccg tttgaagttg ttctatcgtc caaaaggttt gccgggaagc
     1081 aacctcgagg ccatgaaaca gttacgtttc cagtgtcaaa ataaccaaat tgtggttaat
     1141 aacgactccg cttataacct gacctttaat cagttttcca ttaatggaca aaagattgaa
     1201 cgggcgggaa tggttctcgc gaaaggaaag ttaaacatta accttccagc cggtacaggc
     1261 aacgcgcacg aagtgaaata cagcattatt aatgatttcg gtgttgctgg cgaaatgctg
     1321 acgaagccga ttaactgatc cttttacaag gcgcttttac tatgcattgg atgcatttac
     1381 ctctctatca ctacagagcg catttttcct tctctctgct ggcattaacg attgccagct
     1441 cgttacctgc gtatggcgga aaatttaacc ctaaatttct cgaaaacgtt cagggtatcg
     1501 atcaacacat cgacttgtcg gtatacgact cacctgtcgg ccagcaaatc cccggtaaat
     1561 accgggtttc ggtgtttgta aacgaagaaa aaatggcttc ccggacgctg gattttagta
     1621 ccgccagcga agcgaaacgc aaagccagcg gtgagtcgct tatgccttgc cttagccggg
     1681 tgcaactgga agaaatgggc gtgcgcgttg acagctttcc ggcgctgaag atgtcaccac
     1741 cagaagcctg cgtagcgttt gatgaaatca ttccccaggc caccagccgc tttgatttta
     1801 atactcagac gctgcatctt tcctttccac aggcggcaat gatgatgacc gctcgcggca
     1861 cggttgatcc ttctcgctgg gacgaaggaa tacctgcgct gttgctggat tacagcttta
     1921 gtggcagcaa tggacgcaat gaaggtacag gttcatcttc agattcgacc agtgacagtt
     1981 attacctgaa cctgcgcagc ggtttgaacg ttggtccgtg gcgtttacgt aataacagta
     2041 tctggaaccg taccgatggc aaaaaccaat gggataacgt tggtacatcg ttaaaccggg
     2101 caattattcc acttaaatca cagataactc tgggtgatac cgccacgcct ggcgagattt
     2161 ttgatagcgt acaaatgcgt ggggcgttac tggcatccga cgatgaaatg ttgccggaca
     2221 gccagcgtgg ctttgctccg gtagtgcgcg gcatcgccaa aagcaacgct gaagtgagta
     2281 tcgaacagaa cggctatgtc atttatcgca cttttgttca gccgggtgca tttgagatta
     2341 acgatctcta cgcaacctcc ggcagcggcg acctgacggt gatcattaaa gaagccgacg
     2401 gaagtgaaca acgttttatc cagccgtttt cggcggtggc gattttccag cgtgaagggt
     2461 atctgaaata cagtctggct gccggtgaat atcgtgctgg taattacgac agcgataaac
     2521 cacatttcgg gcagtttacc gccatgtacg gcttgccgtg gggtatgacc gcctacggtg
     2581 gtgcattgtt gtcggcagat tacaacgctt tagctctggg cctggggaaa aacttcggta
     2641 ccattggcgc agtatcggtg gatgtcactc aggcgaaaag tcagttacgc aacaatgaaa
     2701 aagaggaagg ccagtcttat cgcttcctct attcgaaaag ttttgagggt ggcactgacc
     2761 tgcgtctgtt gggctacaaa tactcaacca gcggctacta caccttccag gaagcaactg
     2821 atgttcgcag tgacgccgac agtgattacc gccgttatca caagcgcagc cagatacagg
     2881 gcaatattac ccagcaactg ggagactacg gctcggttta tttcaatatg acgcagcagg
     2941 attactggaa cgttgacggt aaggaaaact ccttaagcgc cggttatcac ggtcacattg
     3001 gtcgggtgaa ttacagcgtg gcctatacct ggacgagaag tccggaatgg gaagaagacg
     3061 atcgcttgtg gtcattcagt gtttccatcc ctctgggggg cgcgtggagc agttatcgca
     3121 tgactaccga ccagaatggc aagacgtcgc aacaggcctc tgttagcggc acgttactgg
     3181 aagatcgtaa cctgagctat aacgttcagc agggttacac cagtaatggc gtaggctaca
     3241 gtggcagcgt caacatgggg tatatgggtg gctctggtaa cattgacgta ggctataact
     3301 attctaaaga taaccaacag gttaactatg gcgtgcgcgg cggtgtcatc gttcacagtg
     3361 aaggtataac gctttcgcag cctttaggcg aaagtctggc gattgtttct gcaccgggcg
     3421 cgcgtggtgg acatgtggtt aacagcagcg gcgtagaagt tgactggatg ggcaacgccg
     3481 tggtgcctta tctcacacca tatcgtgaaa ccattgtgga attgcgtagc gacacgttgg
     3541 ggcaaaacgt tgaactacag gaagcgttcc agaaagtcgt gcctactcgt ggtgcagttg
     3601 tgcgttcccg ctttgatacc cgcgtcggtt accgtgtgct gatgagctta aaacaggcga
     3661 atggcaacgc agtaccgttt ggtgcaaccg cagcgttaat tgatgagagc aaacctgcca
     3721 gcagcattgt gggtgaagaa ggacagcttt atatcagcgg gatgccagaa gagggcgaat
     3781 tgcaggtgag ctgggggaac gagcaggcgc aacgttgccg tgtgccattc cgcttgccag
     3841 agaacaagga caatgcggct atcgtgatgg ttaatgcggt atgcgagaaa taaggatatt
     3901 tagatgaaaa ttaaaaattt gttggcaggt atggtgctgt tagggacgag cacttttgct
     3961 ggaaatatat gggctgcgga ttggggacca tgtcagacat caaatggggt ggcccatgag
     4021 tatagttttg atttcgtaca gactatccag gtaccttcag aaaataaggc tgggaaaata
     4081 ttaactcagc cctttgcttt aggtacaaaa tattccgctt attgtgaatg cccggatccc
     4141 attccagata atggagttgt gacttatttt aaaggtgtaa cgcttctgcc tgagcctgga
     4201 actgtagatg ggtactataa attcaataat tacattgata ttctcaccaa aatatatgtc
     4261 tacacacaat tagatatccc ggtgcctttt accgatcgaa gcaatggcac ggcacagaaa
     4321 gaatgtacgc cctatactgc taacaattgg gggacaggtg gtaaaggaag tatcagtatt
     4381 tatatttcac atccttttgt tgggcaaatg attattccta aaacacgagt tgcgtccctc
     4441 tttgggacaa aaaagaaagg tgtttacaat gatagtcagc ccatggccaa tgtcagtatt
     4501 tccgggagta ttacagttac tcaaggctgt gaacttgccg ctggcaccgt actggatatt
     4561 ccattcggag aatatcaggc ccatgatttt aaagggcgtg ccggacaacc accacaaaat
     4621 gttcagaagg tacaaaaaga attaagcttt gactgcaata atatttcaga tggcgtcaaa
     4681 atttatctca gtattgatgc aacaccaaat actgcctatc ccagcgcaat tgatttaggc
     4741 aacgccgatg ttggtgcagt cattgaggac ggtaaaggta atattcttaa gccgaatgac
     4801 agtaatagct tattagaaat gaacccaggc tcgctgtatg aagatgtcaa acgcaaagcg
     4861 acaaccacaa ttaccgctta tcccgttagc actaccggaa aattacccgc tgccggggat
     4921 tatagcggta ttgctaccat acatgtcgaa ctggagtaac agtcatgaag cgaacaatac
     4981 ttgcgttatg cctgctgcct ctttcctttt cgtcattcgc ggcaaccacg gatttagggc
     5041 cgaaaggaac attgaaattc acccttaaaa tcagccaggg tgcttgcgaa ctggtgaaag
     5101 acagcgttga ggttgacatg ggcaccgcgg ttctcaaaaa gccggtaaga atcggaacgg
     5161 aaattaatcc cactcctttt tccatcggcc tgaaaaactg tagcgaagtg gtcagagcct
     5221 acgtgactat ggacggtaca cccaatgcag acgactcaaa tctttttgcg ctggatgcgg
     5281 gtggcgctac aggcgttggg ctgaaaatca aaaccgctgc tggagtacaa cagttcccgg
     5341 aaaatacaac gccgacaccg gttgagtttg caattaacgg tgatggcgca catcaactga
     5401 actatatcgc cagctacgtg cctattcgcg ccgatgcaac tattggtcgc gcagatgcaa
     5461 cggtcgactt tactgtacag tacgaataat ttcacatctt aagggccagt taactggccc
     5521 tttttactgc tttttgtaac gctaaagtct cttttcaaac ttgcattttt gtaaatttgt
     5581 gcttcatgca cactctttcc ccacactttt tcgctttgct gtggtctact tattcgcgcg
     5641 tgtagatttt acttatctga ctacctccgc acttttcccc tgccgggcct gaaaagccgc
     5701 taagcagggt gttatcacct gtttgtccag ggtttgtttg catgagatac atcaaatcga
     5761 ttacacagca gaagctgagc tttttgcttg caatctatat tggccttttt atgaatggcg
     5821 cggtttttta tcgccgcttc ggcagctatg cgcacgattt taccgtctgg aaaggcgttt
     5881 ctgctgttgt tgaactggcc gccaccgtac tggtgacctt ctttttacta cgtcttctat
     5941 cgctgtttgg ccgccgcagc tggcgtattc tggcgtcgct ggtggtgctc ttttccgcag
     6001 gtgccagcta ttacatgacc ttccttaatg tggtcattgg ttatggcatc atcgcttccg
     6061 tcatgaccac cgatatcgac ctgtcaaaag aagttgttgg tctgaacttt attctctggt
     6121 taatcgccgt tagtgcattg cctcttatcc ttatctggaa taaccgctgt cgctacacct
     6181 tgctccgaca actgcgaacc ccagggcagc gtattcgcag cctggcggtc gtcgtactgg
     6241 cgggtattat ggtttgggca ccgattcgtt tgctggatat ccagcagaag aaagtggaga
     6301 gggcgaccgg cgttgatttg ccgagttatg gcggtgtcgt agcgaactct tatctgccat
     6361 caaactggct ttctgcgttg gggctgtatg cctgggcgcg ggtcgatgaa tcttccgata
     6421 ataattcatt gcttaatccg gcgaagaaat tcacttatca ggcaccgcaa aacgttgatg
     6481 acacttatgt cgtgtttatc atcggtgaaa ccacgcgttg ggaccatatg ggtattttcg
     6541 gctatgagcg taataccacg ccgaaactgg cccaggagaa aaatctggcg gcgttccgtg
     6601 gttactcctg tgataccgca accaaactct cactgcgttg catgtttgta cgtcaggggg
     6661 gcgcggaaga taatccgcag cgcacattaa aagaacagaa cattttcgcg gttctgaagc
     6721 agttaggatt cagttctgac ctctacgcta tgcagagcga aatgtggttc tacagcaaca
     6781 cgatggcgga caacattgct tatcgtgagc agattggtgc ggagccacgt aatcgtggca
     6841 agccggtaga tgatatgttg ctggtagacg aaatgcagca atcgctaggg cgcaacccgg
     6901 atggtaagca tctgatcatt ctgcatacca aaggttcgca ttttaactac acccagcgtt
     6961 atccgcgtag cttcgcgcag tggaagccgg aatgtattgg tgttgatagc ggctgtacca
     7021 aagcgcagat gatcaactcc tatgacaact cggtgaccta tgtggatcac tttatctcca
     7081 gcgtgattga tcaggttcgc gataagaaag cgattgtgtt ctacgcagct gaccacggtg
     7141 agtcaattaa tgaacgcgag cacctgcacg gcacgccgcg tgaactggca ccgccggagc
     7201 agttccgcgt accgatgatg gtctggatgt cagataaata tctggaaaat ccggccaatg
     7261 cgcaggcatt tgcgcagctg aaaaaagaag ccgacatgaa agtgccacgc cgtcacgtag
     7321 agctgtacga taccatcatg ggttgtcttg gctatacttc accggatggt ggaattaacg
     7381 aaaacaacaa ctggtgtcac atcccgcagg caaaagaggc agcggctaac taagcggcct
     7441 gctgactttc tcgccgatca aaaggcattt tgctattaag ggattgacga gggcgtatct
     7501 gcgcagtaag atgcgccccg cattcggtga ttggcgcagc ctggtagcgc acttcgttcg
     7561 ggacgaaggg gtcggaggtt cgaatcctct atcaccgacc aaattcgaaa agcctgctca
     7621 acgagcaggc ttttttgcat ctgcagctca tgaggatgag aacctccggg ggcagggagg
     7681 ttcgactcga cgtaggcctg ataagacgcg tcagcgtcgc atcaggcaat ggcaccgcac
     7741 ttattgtcgg atgcggcgtg aacgccttat tcgacctaca cgccacacat ttccctgcca
     7801 aatttgcgcc ccatatctca ctatccatta acggatttgt gacagaaacc attgtctttt
     7861 tttatatttg cttaaatctt tttttgcgtt acttatggct ggcgctggca ttttccgctg
     7921 tgcgttttag cgttcatggt attaatcgct cagatatcca ccttttcctc agattttttt
     7981 cactaaatgc ataaaactta atcaccaaat gttgctaaat attaagctga ttgttctggt
     8041 catgttggag tagcacaaat ttctctgctg caaatagctg tttgaagttt ttttaatctt
     8101 tgtttgtgga ttctcaccgt tggtgtaaat cccggttggc tgtattgacg ttttcacatt
     8161 ctgttgacag attgtaggtc acgaggggca ttttatggag gatccgcact gttacactga
     8221 tgttaattag tacggcatcc ccacctcata acgttgaccc gaccgggcaa aaaacaaaaa
     8281 aggtcaggca gcgacaaccc actgcaaagg gttaaaacaa caaacatcac aattggagca
     8341 gaataatgcg tatttccttg aaaaagtcag ggatgctgaa gcttggtctc agcctggtgg
     8401 ctatgaccgt cgcagcaagt gttcaggcta aaactctggt ttattgctca gaaggatctc
     8461 cggaagggtt taacccgcag ctgtttacct ccggcaccac ctatgacgcc tcttccgtac
     8521 cgctttataa ccgcctggtt gaatttaaaa tcggcaccac tgaagtgatc ccgggcctcg
     8581 ctgaaaagtg ggaagtcagc gaagacggta aaacctatac cttccatctg cgtaaaggtg
     8641 tgaagtggca cgacaataaa gaattcaaac cgacgcgcga actgaacgcc gatgatgtgg
     8701 tgttctcgtt tgatcgtcag aaaaacgcgc aaaacccgta ccataaagtt tctggcggca
     8761 gctacgaata cttcgaaggc atgggcttgc cggagctgat cagcgaagtg aaaaaggtgg
     8821 acgacaacac cgttcagttt gtgctgactc gcccggaagc gccgttcctc gctgacctgg
     8881 caatggactt cgcctctatt ctgtcaaaag aatatgctga cgcgatgatg aaagccggta
     8941 caccggaaaa actggacctc aacccaatcg gcaccggtcc gttccagtta cagcagtacc
     9001 aaaaagactc ccgtatccgc tacaaagcgt ttgatggcta ctggggcacc aaaccgcaga
     9061 tcgatacgct ggtcttctct attactcctg acgcttccgt gcgttatgcg aaattgcaga
     9121 agaatgaatg ccaggtgatg ccgtacccga acccggcaga catcgctcgc atgaagcagg
     9181 ataaatccat caacctgatg gaaatgccgg ggctgaacgt cggctacctc tcctataacg
     9241 tgcagaaaaa accgctggat gacgtgaaag ttcgccaggc actgacctac gcggtgaaca
     9301 aagacgcgat catcaaagcg gtttatcagg gcgcgggtgt atcagcgaaa aacctgatcc
     9361 cgccaaccat gtggggctat aacgacgacg ttcaggatta cacctacgat cctgaaaaag
     9421 cgaaagcctt gctgaaagaa gcgggtctgg aaaaaggttt ctccatcgac ctgtgggcga
     9481 tgccggtaca gcgcccatat aacccgaacg ctcgccgcat ggcggagatg attcaggctg
     9541 actgggcgaa agtcggcgtg caggccaaaa ttgtcaccta cgaatggggt gagtacctca
     9601 agcgtgcgaa agatggcgag caccagacgg taatgatggg ctggactggc gataacgggg
     9661 atccggataa cttcttcgcc accctgttca gctgcgccgc ctctgaacaa ggctccaact
     9721 actcaaaatg gtgctacaaa ccgtttgaag atctgattca accggcgcgt gctaccgacg
     9781 accataacaa acgcgttgaa ctgtacaagc aagcgcaggt ggtgatgcac gatcaggctc
     9841 cggcactgat catcgctcac tccaccgtgt ttgaaccggt acgcaaagaa gttaaaggct
     9901 atgtggttga tccattaggc aaacatcact tcgaaaacgt ctctatcgaa taattaaaag
     9961 ccatacccgg tggcgctgtg cctctgcgtg cagcgccatc cggcagcaat acttcattcc
    10021 cagcccgacc tgggcgggaa ttgatttgtg agcaatacag acacgcagtt ccaggctgcg
    10081 ggtcactata gagaatccgg gttatgttgc agtttattct ccgacgtttg ggactcgtca
    10141 tccccacgtt tatcggtatt acccttctca catttgcctt tgtccacatg atcccgggcg
    10201 atccggtgat gatcatggcg ggcgaacgtg ggatctcccc agagcgtcac gcgcagctgc
    10261 tggctgaact cggcttagat aaaccgatgt ggcagcagta tctccattac atttggggcg
    10321 ttatgcatgg cgatctaggc atttcaatga aaagccgcat cccggtttgg gaagagttcg
    10381 tgccgcgcct ccaggccacg ctggaacttg gcgtctgcgc gatgattttt gcgactgcgg
    10441 tcggtattcc ggtcggcgtg ctggctgcgg ttaaacgcgg ttccattttc gatcacacag
    10501 cggttggcct ggcgctgacc ggctactcga tgccgatctt ctggtggggc atgatgctga
    10561 tcatgctggt ttcggtgcac tggaacctga cgcccgtctc cggtcgcgtg agcgatatgg
    10621 tgttcctcga tgactccaat ccgttaaccg gttttatgct aatcgacacc gccatctggg
    10681 gtgaagacgg caactttatc gatgccgtcg cccatatgat cttgcctgcc attgtgctgg
    10741 gtactattcc gctggcggtc attgtgcgta tgacacgctc ctcgatgctg gaagtgctgg
    10801 gcgaggatta catccgcacc gcgcgcgcca aagggctaac ccgcatgcgg gtgattatcg
    10861 tccatgcgct gcgtaacgcg atgctgccgg tggtgaccgt tatcggcctg caggtgggaa
    10921 cattgctggc gggggcgatt ctgaccgaaa ccatcttctc gtggcccggt ctgggacgct
    10981 ggttgattga cgcactgcaa cgccgcgact atccggtagt gcagggcggc gtattgctgg
    11041 tggcgacgat gattatcctc gtcaacttgc tggtcgatct gctgtacggc gtggtgaacc
    11101 cgcgtattcg tcataagaag taaggggaca tcatgtcaca ggttactgaa aataaagtga
    11161 ttagcgcacc ggtgccgatg accccgttac aggagttctg gcactatttt aaacgcaaca
    11221 aaggcgcggt cgtcgggctg gtttacgtcg tcatcgtgct gttcatcgcg atctttgcca
    11281 actggattgc accctataac ccggcggaac agttccgcga tgcactgctc gccccgccag
    11341 cctggcagga aggcggcagc atggcgcact tgctgggcac cgatgacgta ggccgtgatg
    11401 tgctgtcgcg cctgatgtac ggtgcgcgcc tgtcgctgct ggttggctgt ctggtagttg
    11461 tgttatcgct gattatgggc gttattctcg gcctgatcgc cggttacttt ggcggcctgg
    11521 tcgataacat cattatgcgc gtggtcgata tcatgctggc gctgccaagt ctgctgctgg
    11581 cgctggtgct ggtggcaatt ttcggcccgt caattggtaa cgccgcgctg gcactgacct
    11641 tcgttgcctt gccgcactat gtgcgcttaa cccgcgccgc cgtgctggtg gaagttaacc
    11701 gcgattacgt caccgcgtct cgcgtggcgg gtgccggggc gatgcgtcag atgtttatta
    11761 acatcttccc gaactgcctt gcgccgctga ttgttcaggc gtcgctcggt ttctctaacg
    11821 ccattctcga tatggctgct cttggtttcc tcggcatggg ggcacagccg ccaacgcctg
    11881 agtggggcac catgctctcc gacgtgttgc agttcgcgca aagcgcctgg tgggtcgtga
    11941 ccttcccggg tctggcgatc ctgctgacgg tgctggcatt taacctgatg ggtgacggtc
    12001 tgcgtgacgc gctcgatccc aaactgaagc agtaagaggt tcgagatggc gttattaaat
    12061 gtagataaat tatcggtgca tttcggcgac gaaagcgcgc cgttccgcgc cgtagaccgc
    12121 atcagctaca gcgtaaaaca gggcgaagtg gtcgggattg tgggtgagtc cggctccggt
    12181 aagtcggtca gttcactggc gattatgggg ctgattgatt atccgggccg cgtaatggca
    12241 gaaaaactgg agtttaacgg ccaggatttg cagcgtatct cagaaaaaga gcgccgcaac
    12301 ctggtgggtg ccgaagtggc gatgatcttc caggacccga tgaccagcct taacccgtgc
    12361 tacaccgtgg gtttccagat tatggaagcg attaaggtgc atcagggcgg caacaaaagt
    12421 acccgccgtc agcgagcgat tgacctgctg aatcaggtcg gtattcccga tccggcatcg
    12481 cgtctggatg tttacccgca tcagctttcc ggcggcatga gccagcgcgt gatgatcgcc
    12541 atggcgattg cctgtcggcc aaaactgctg attgccgatg aaccgaccac cgcgctggac
    12601 gtgaccattc aggcgcaaat catcgaacta ctgctggagc tacagcagaa agagaacatg
    12661 gcgctggtgt taattaccca tgacctggcg ctggtggcgg aagcggcaca taaaatcatc
    12721 gtgatgtatg caggccaggt ggtggaaacc ggtgatgcgc acgccatctt ccatgcgccg
    12781 cgtcacccgt atactcaggc attgctgcgt gcgctgccag aatttgctca ggacaaagaa
    12841 cgtctggcgt cgttgccagg tgtcgttccc ggtaagtacg accgcccgaa cggctgcctg
    12901 cttaacccgc gctgccccta tgccactgac agatgtcgcg ctgaagaacc ggcgctgaat
    12961 atgctcgctg atgggcgtca gtccaaatgc cattacccac ttgatgatgc cgggaggccg
    13021 acactatgag tacgcaagag gccacctcgc aacaaccgct gttgcaggct atcgacctga
    13081 aaaaacatta tccggtgaag aaaggcatgt tcgcgccgga acgtctggtt aaagcgctgg
    13141 atggcgtttc gtttaacctt gaacgtggca aaacgctggc agtagtgggc gaatctggct
    13201 gcggtaaatc gaccctcggt cggttgctga cgatgattga aatgcccacc ggtggcgagc
    13261 tgtattacca ggggcaggat ctgcttaagc acgatccgca ggcgcagaag ctgcgtcggc
    13321 agaaaatcca gatcgtcttc cagaaccctt acggttcgct gaatccgcgt aaaaaagttg
    13381 ggcaaattct cgaagagccg ctgcttatta acaccagctt aagcaaagaa cagcgtcggg
    13441 aaaaagccct gtcgatgatg gcgaaagtcg gcctgaaaac cgagcactat gaccgctatc
    13501 cgcatatgtt ctccggcgga caacgtcagc gtatcgctat tgcccgtggt ctgatgctcg
    13561 acccggatgt ggtgattgcc gatgagccgg tttctgcgct ggacgtctca gtgcgtgcgc
    13621 aagtgctgaa tctgatgatg gatttacagc aggaactggg gctttcttat gtctttatct
    13681 cccacgacct gtcggtggtg gagcacattg ccgatgaagt gatggtgatg tacctgggcc
    13741 gctgcgtgga gaagggaacg aaagaccaaa tcttcaataa cccgcgccat ccgtacactc
    13801 aggcgctact ctccgcgacg ccgcgcctga acccggacga tcgccgcgaa cgcatcaaac
    13861 tcaccggtga actgccaagc ccgctgaatc caccgccggg ttgcgccttc aacgcccgct
    13921 gtcgtcggcg cttcggcccc tgcacccagt tgcagccgca gctaaaagac tacggcggtc
    13981 aactggtagc ttgttttgct gttgatcagg atgaaaatcc gcagcgttaa ctcacaaaac
    14041 cggggccatg cgctccggtt tactcaatca gcttcaacag tggcgacaag catagcaaaa
    14101 tgccgtaaag cagaattagc caggctttaa atccgcgaag tttttccagt tgtgcgactt
    14161 tatagatcag gaaaaacgga atgaggcacg acacaatgcc atataacggg cttcccaact
    14221 gaaagaacac cagcaccgat acacgaaacg aaacccaaat cgtcaatgta atgacgataa
    14281 aagcgcagat cgccagagtc agcatgcgtg agttaatttt cttggtatca ataattcggc
    14341 ttaacagatt gagaataatg cctttaatag cctcgtggaa accgagataa atgccaaaga
    14401 atgcggtcag tacggcaaag atattcagca ccgtagaggt gatatgaatg atatgtccag
    14461 ggatcacctg cgcggccagc gccagtgccg agatattttg ttcaaaggca gaaacggctt
    14521 cttcgtggct aattgagaag gtaaacgaaa aggcaaaaaa caggatcacc gcgatgagcg
    14581 tgatataact aattcggtgg gtacgcagcg cgagccgggt tgccagtact ttatccgctt
    14641 cccgtttacg ataggcaata ttcattgggt taagtacctg aataaatact gcagaaaaga
    14701 agcaaaatgg aatggtaagc aagacatcgc ggaaaaagac ggaggcttgc gggaaggcgg
    14761 ttatattggc gaaattccag tgcgggatca tcgcaaaacc gaacacgaca ataatcccta
    14821 ctttgaccac caccattggc ccggaaatct taaacagtaa tcgttcacca ccagacgcaa
    14881 tcgccaccag tacggcgaaa atagcgactt tataaagtag agattgtgaa agatcggcat
    14941 cggttaaacc gaaggttttc aggtacgagg cgctgtcgaa aaccacggag agagagtaga
    15001 taaaaatccc gtggataatc atcaaaaagt agataacccc gaggaaaatt ccccagttct
    15061 tccccaggta atgactgata atatcggtgt agtcattaca ggaatcgctt tcagaaaggg
    15121 tttttaaata aatgtcctgc actacccagg tggcaggata agcaatgatc gctgcggtaa
    15181 taaataccca aattcccttc aagccaattt gtactggcat cagcacggtt ccggcaccaa
    15241 tcgccatgcc tatgcataat aaaacccagc cgaagtcgta gcgtgtaaac ggcaatttct
    15301 gattgtgttt cgatagtgtg ttgtgctgca taaagtcctg tccagaaaga agtcggcctc
    15361 gcaaaaatga ataaacccag tttctcttgc gcaacaggtg gagaagtcga atacgcagta
    15421 tgtaaaaaat gaggcaacag attcactggg gaggtcgcga ggaggcgcaa ttctacaaaa
    15481 ttgcgcattt cactaccagt ctggagtcag cccggatatg aactgcaact attgcaaata
    15541 aagaaattaa gctggaaggc atcgcgcaat gaggaagatt gccgggtgct gacagtaaaa
    15601 agcaatcgta aaatcagcgg caagccgaaa gggttccggt gaggcgcaat gttgcggggg
    15661 ctttatccct ggtggcattg gttgctggag agagaaaacc cccgcacgtt gcaggtatgt
    15721 acctgacaac ataacggggg ctaatcttga acctagacca ctcaagaata gccgcgaaac
    15781 gttgtcatta caacacaggc ggctatatga cgctcgcaga gctgggcatg gccttctggc
    15841 atgatttagc ggctccggtc attgctggca ttcttgccag tatgatcgtg aactggctga
    15901 acaagcggaa gtagcgtgtc atgtgggcgt caggctgccg taatggcaat ttgcgcccgg
    15961 accaggccgc aggagtgaaa ctctgcggcc tttttcgtaa gaaaaatgca acggttgcaa
    16021 gcggataata ttctgcggaa tgcgcagcac agagtgaaga tgagacggat tccggaacag
    16081 aaaaacagtc gtagaatcag cgtcaagccg gaaggattcc ggtgaggcgc aatgttgcgg
    16141 gggcttaatc cctggtggca ttggttgctg gagagagaaa acccccgcac gttgcaggta
    16201 tgcacctgac aacaccacgg gggccattct tgcatccaaa caatacaaga atagccgcga
    16261 aacgttgtca ttacaataca ggcggctata tgacgctcgc agaactgggc atggccttct
    16321 ggcatgattt agcggctccg gtcattgctg gtattcttgc cagtatgatc gtgaactggc
    16381 tgaacaagcg gaagtaacgt gctatgcggg cgtcaggctg ccgtaatggc aatttgcgcc
    16441 cggatcaggc cgcaggggtg aaactctgcg gcctttttcg taagaaaaat gcaacggttg
    16501 caagcggata atattctgcg gaatgcgcag cacagagtga agacgtgacg gattccggaa
    16561 cagaaaaaca gtcgtagaat cagcggcaag ccggaagggt tccggtgagg cgcaatgttg
    16621 cgggggcttt atccctggtg gcattggttg ctggagagag aaaacccccg cacgttgcag
    16681 gtatgcacct gacaacatca cgggggccat tcttgaacct gaacaactca agaatagccg
    16741 cgaaacgttg tcattacaac acaggcggct atatgacgct cgcagaactg ggcatggcct
    16801 tctggcatga tttagcggct ccggtcattg ctggcattct tgccagtatg atcgtgaact
    16861 ggctgaacaa gcggaagtaa cgtgtcatgc gggcgtcagg ctgccgtaat ggcaatttgc
    16921 gcccggacca ggccgcaggg gtgaaactct gcggcctttt tcgtaagaaa aatgcaacgg
    16981 ttgcaggcgg ataatattct gcggaatgcg cagcacagag tgagtaagtg atggatttcg
    17041 gaacagaaaa acagtcgtag aatcagcggc aagccggaaa ggttccggtg aggcgcaatg
    17101 ttgcgggggc tttatccctg gtggcattgg ttgctggaga gagaaaaccc ccgcacgttg
    17161 caggtatgca cctgacaaca taacgggggc taatcttgaa cccaaacaac tcaagaatag
    17221 ccgcgaaacg ttgtcattac aacacaggcg gctatatgac gctcgcagag ctgggcatgg
    17281 ccttctggca tgatttagcg gctccggtca ttgctggcat tcttgccagt atgatcgtga
    17341 actggctgaa taagcggaag taacgtgtca tgcgggcgtc aggctgccgt aatggcaatt
    17401 tgcgcccgga tcaggccgca gaggttaaac tctgcggcct ttttcgtgct tactgcgggt
    17461 aaggcaccca gtcgccgccg ttcaggcgaa cgtacggttt atcctggtat tgaataacta
    17521 ctgcatttga gttctcggag accggtgctg tttgtggcaa cccactggtg agttttttcc
    17581 agtcaacatt gtcttcggtg aaaatcttgc catcaagaac gcgaaccacc agatcggaga
    17641 tagccaggaa gctgctcggt tggtcaatga caatcggtgc cccctgatgt ggtgccttca
    17701 tgccgaagaa tttcacccca acggggacgt ctgtgataga cgggctaggg atatcacgta
    17761 ggccagatac ctgcattctg tcgcccttca gcgcgccgcc gtgttccggc accacgacca
    17821 ccatcacttt acgacccgat ttctccagtt cagtaaagaa ggcgtccagt tcatcaaaga
    17881 atttctgcgc ccgcgctttg taatccgctg ttttgctgac ccccggataa tggttgccgt
    17941 catgcagtgg aagcgtgttg tagaacgtgg cactacggct gtttttatct ttttcggtaa
    18001 cgtccagcca gcggttaagc acggcggtat cgtcataaac cggcgaacca tcaaagccca
    18061 gcaaaataac cggcagattt gtttgatcca tcaattcagt ctgcatgccg ccattttcgc
    18121 gaacttcttt caaaaaaccg ccgaactggc cgttatgccc catcatcagg tgctgggtaa
    18181 agcccagttt cgaaaggtta tcaaacagat agcagtcgtt atttgccggt tgatacagat
    18241 tagtgtgcga agtctgcccg cagctggcgc gcagtaaacg gatcgccgcc gggccactgt
    18301 aggaggtggc ggagttaaag ttcttgaact caatatcgaa atgcgaccac agtggatgcg
    18361 acatcaaccc ggcggcttct atatccgacc aggaaagcga acagatgtta atcaccagta
    18421 gttcaaatgg ctgagcatca gcgggcagcg aagacgggaa ggtcgattta cgtttcgcct
    18481 ccgcgttata gaaattattc agccaggcgt taaggttcgc cgttgttggc ggtgcagttt
    18541 gtgcgggcat atcacccact accggtgcgc cacccgtcgc cgcaacggtt gccgctgcgt
    18601 taccacccgt cgttgttaca gtggtcgtcg gttgtccggc tggccacaag gagaaacttg
    18661 gtcccgccag ggtaagtacg ttcagccata gcagtatggc aaccacaaaa acggtaatgc
    18721 gaatccattg tgacaggaat aaccaggcca ctaataaaac aaaaatggcc ccaatcatct
    18781 gccagttaat aaagcgtgtg acaaggtcga ttaaataatc ggtactgaac cccgccacct
    18841 gcgaaccctg gctcattatg ctttccgggc caggcaacca ggtgtcatgc cagaacaaag
    18901 caaagccgat cggcagggca atccagtggc gcaagcgatg caggctgtag cgcggaatgg
    18961 gcatcagcag aaacgcggca aacaccaaat tgaggagcgg atggaagtta agatatcccg
    19021 cccacaacag gccgaactta accagaaaat agaagttcca gccggaaagg ccgcgccagt
    19081 attgccagag ggaagaaggc atggcggtat tttgcgtaaa ttgagtcatt ttttggttgc
    19141 cctggctttt tccgtgcggc gtaacgtccc ggccggttta acataacgag gtttagcaaa
    19201 gaacaaacgt aaggtgtcgc gaatgcgtcg caaagagtgc cgcgccagat agcccagcgg
    19261 gaaaaatatc agtgcgcaaa cgacaataat ttcaatgata tcgctgatgg tcatcatgat
    19321 gagcgctcca cagcatcatc taacagtcgc atgggttctg gtattcgtcg ccagtggcga
    19381 ccatcgtgct cggcattgat gaccggttta gaactttgcg ttaaaggcag cggcatgccc
    19441 cattgttctg gggcaagcaa gcgcatctgc accagctcgg cactgatttg atcatcttca
    19501 aaccagacca tacggtttga gaaaatgtcg ccagtaggca atgggaaaat atgattcaac
    19561 gcggtatcca gatcgttaat ccgacagaat gagagaaaca gcaccagccg attaccgcca
    19621 atggtcatga tatcgccggt acggttaggg cgacacagcg tcagggcttg ttcaacgcgg
    19681 atacccggta ccggacgtag ggcaaccaga acgcctttac cgtgggcagg taatagaggg
    19741 ttattcatca tgttgttgac ggcattacag aacacatccc acttctggaa accacgcagt
    19801 ttgagcggct gggtcattga cagcaaggta gtgatatctt ccggcacata gcgactaaac
    19861 ttctgccctt gcacgctttc gatcatcgtc agacaacggg agagtggcgc attccacgga
    19921 ataaccatat ttgcaccgca ggccaataac aaacgttcat cggtggcgcg caggctagcg
    19981 gtattttccc gcacgaggat tttcatcgca ctaccgcgct ggcgacgcag ggtatgaatg
    20041 ctgcgggcca gtggctcgat ttgcgcattt tgctgtaaag aaaagaccac cgtcgccgcc
    20101 tgagcggtac gggcttcatt gaacaggact tcgttattgt tgaacagttg ccagtgttcc
    20161 gatagcggcg gcgcaccttc cagtacagca acattactca gaatgcgttt ttcgtcgctg
    20221 cgtggttgga tctccgcctc ttcgctttga actaatgtcc agataccatt ttgttgctga
    20281 acgctaagct gctgacgggc gctgacccct ttttcgttgc accagaaggc aatatccagc
    20341 aaatgttggt caccctgaaa acgcaaactg gcaagaccaa aaagtgaacg gtattcctca
    20401 agcaacaatg aaaattgttt atcgttatta tttccgggat taattaccaa aagcgaacaa
    20461 tggtttaacc tgctccattt attcatttta tccaaccatg agcgaagccg ctcggcagga
    20521 atgttttgcc atgcgttatt tgcgcaaaca agaataaaaa ggtaattatg gggatcaata
    20581 gaacactgca aatcacgggt caaatagtat agaccttttt catgatttag cattgaaaat
    20641 aattttattt tttccggccc ttgagaatca tctaattgaa agattttcgc cggatcgctg
    20701 tccatgctaa tgaccgcgac gtgtgcggtt tcagcctggg atgcaattgt ttgattcgcc
    20761 agactgatag catcttcatg gcgatcgacg ttaaaccacc agacgccgcc tgctggcata
    20821 tgtcgcagct catcccataa tgatgagata ccgatagaga atacagggtc cacaatgtcc
    20881 ctcatgtcac cattttgtct gattatcagt ttactagcga aagctaagaa ataaacctac
    20941 cattgaaatt aaaacttatc agatttagca tgtgaatcaa tttagaaggt cacaagacgt
    21001 gtgattttag tcttttcgtt tttaactttt tctgttgcgt aacggaaagt caaaaagtga
    21061 gcaaattccc gtctcgccgt aaacaataaa ccggcgaaat gctcacggtt agaattgtct
    21121 catgaacggt acggttattt catagggatc aagcaaaatg aataacaatg aaccagatac
    21181 tctgcctgat cccgcgatag gctatatctt ccagaatgat attgtggcgt taaagcaggc
    21241 attttcactg cctgatattg attatgccga tatttcccaa cgcgaacagt tggccgcggc
    21301 attaaaacgc tggccgttgc tggcagagtt tgcgcaacaa aagtagggga ttggtgaatg
    21361 gccgtactgg gattgcaggg ggtgcgggga ggcgtgggga caacaaccat caccgccgca
    21421 ttagcctggt cattacaaat gttgggagaa aatgttctgg tggtcgatgc ctgcccggac
    21481 aacttgttgc gcctgtcatt taatgttgat tttacccacc gtcagggctg ggccagagcg
    21541 atgctggatg gccaggactg gcgtgacgct gggttgcgct acacctcgca gctcgatttg
    21601 ctgccttttg gtcagttatc cattgaagaa caagaaaatc cacagcactg gcaaacccgg
    21661 ctgagcgata tttgctccgg cttacagcaa ctaaaagcca gcgggcgtta ccagtggatt
    21721 ttaatcgact taccgcgtga tgcctcgcag ataacccacc agctgctgag tttgtgcgat
    21781 cactcgctgg caatcgtcaa tgtggatgcc aactgccata tccgactgca tcagcaagcg
    21841 ctgccggatg gcgcacatat tttgattaat gacttccgta ttggcagtca ggttcaggac
    21901 gatatttacc agctttggtt gcaaagccag cgccgattac tgccgatgct cattcatcgt
    21961 gatgaagcga tggctgaatg cctggcggct aagcaaccag taggtgaata tcgcagtgat
    22021 gcgctggcgg ctgaagagat actgacgctg gcgaactggt gcctgttgaa ctactccggg
    22081 ctgaaaacgc cagtcgggag tgcatcatga gtatcctgac ccggtggttg cttatcccgc
    22141 cggtcaacgc gcggcttatc gggcgttatc gcgattatcg tcgtcacggt gcgtcggctt
    22201 tcagcgcgac gctcggctgt ttctggatga tcctggcctg gatttttatt ccgctggagc
    22261 acccgcgctg gcagcgtatt cgcgcagaac ataaaaacct gtatccgcat atcaacgcct
    22321 cgcgtccgcg tccgctggac ccggtccgtt atctcattca aacatgctgg ttattgatcg
    22381 gtgcatcgcg caaagaaacg ccgaaaccgc gcaggcgggc attttcaggt ctgcaaaata
    22441 ttcgtggacg ttaccatcaa tggatgaacg agctgcctga gcgcgttagc cataaaacac
    22501 agcatctgga tgagaaaaaa gagctcggtc atttgagtgc cggggcgcgg cggttgatcc
    22561 tcggtatcat cgtcaccttc tcgctgattc tggcgttaat ctgcgttact cagccgttta
    22621 acccgctggc gcagtttatc ttcctgatgc tgctgtgggg ggtagcgctg atcgtacggc
    22681 ggatgccggg gcgcttctcg gcgctaatgt tgattgtgct gtcgctgacc gtttcttgcc
    22741 gttatatctg gtggcgttac acctctacgc tgaactggga cgatccggtc agcctggtgt
    22801 gcgggcttat tctgctcttc gctgaaacgt acgcgtggat tgtgctggtg ctcggctact
    22861 tccaggtagt atggccgctg aatcgtcagc cggtgccatt gccgaaagat atgtcgctgt
    22921 ggccgtcggt ggatatcttt gtcccgactt acaacgaaga tctcaacgtg gtgaaaaata
    22981 ccatttacgc ctcgctgggt atcgactggc cgaaagacaa actgaacatc tggatcctcg
    23041 atgacggcgg cagggaagag tttcgccagt ttgcgcaaaa cgtgggggtg aaatatatcg
    23101 cccgtaccac tcatgaacat gcgaaagcgg gcaacatcaa caatgcgctg aaatatgcca
    23161 aaggcgagtt cgtgtcgatt ttcgactgcg accacgtacc aacgcgatcg ttcctgcaaa
    23221 tgaccatggg ctggttcctg aaagagaaac agctggcgat gatgcagact ccacaccact
    23281 tcttctcgcc ggacccgttt gaacgcaacc tggggcgttt tcgtaaaaca ccgaacgaag
    23341 gcacgctgtt ctatggtctg gtgcaggatg gcaacgatat gtgggacgcc actttcttct
    23401 gcggttcctg tgcagtaatt cgtcgcaaac cattggatga aattggcggt attgctgtcg
    23461 aaacggtaac cgaagatgcg cacacgtctc tgcgcctgca ccgtcgtggc tacacctctg
    23521 catatatgcg tattccgcag gcggcggggc tggcgaccga aagtctgtcg gcgcatatcg
    23581 gtcagcgtat tcgctgggca cgcgggatgg tgcaaatctt ccgtctcgta tagcgcggcg
    23641 atttttttga taccgatttg catttcattt gatactg
//
LOCUS       DAZFQD010000075        23359 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.075, whole genome shotgun sequence.
ACCESSION   DAZFQD010000075 DAZFQD010000000
VERSION     DAZFQD010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23359
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.075"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            53..1831
                     /gene="ygeV"
                     /locus_tag="ACHKTW_002951"
     CDS             53..1831
                     /gene="ygeV"
                     /locus_tag="ACHKTW_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFO6463939.1"
                     /translation="MELATTQSVLMQIQPTIQRFARMLASVLQLEVEIVDENLCRVAG
                     TGAYGKFLGRQLSGNSRLLRHVLETKTEKVVTQSRFDPLCEGCDSKENCREKAFLGTP
                     VILQDRCVGVISLIAVTHEQQEHISDNLREFSDYVRHISTIFVSKLLEDQGPGDNISK
                     IFATMIDNMDQGVLVVDDESRVQFVNQTALKTLGVVQNNIIGKPIRFRPLTFESNFTH
                     GHMQHIVSWDDKSELIIGQLHNIQGRQLFLMAFHQSHTSFSVANAPDEPHIEQLVGEC
                     RVMRQLKRLISRIAPSPSSVMVVGESGTGKEVVARAIHKLSGRRNKPFIAINCAAIPE
                     QLLESELFGYVKGAFTGASANGKTGLIQAANTGTLFLDEIGDMPLMLQAKLLRAIEAR
                     EILPIGASSPIQVDIRIISATNQNLAQFIAEGKFREDLFYRLNVIPITLPPLRERQED
                     IELLVHYFLHLHTRRLGSVYPGIAPDVVEILRKHRWPGNLRELSNLMEYLVNVVPSGE
                     VIDSTLLPPNLLNNGTTEQSDVTEVSEAHLSLDDAGGTALEEMEKQMIREALSRHNSK
                     KQVADELGIGIATLYRKIKKYELLNT"
     gene            complement(1871..2350)
                     /gene="xdhC"
                     /locus_tag="ACHKTW_002952"
     CDS             complement(1871..2350)
                     /gene="xdhC"
                     /locus_tag="ACHKTW_002952"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HFO6463940.1"
                     /translation="MNHSETITIECTINGMPFQLHAAPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWAEGKEIRTLEGEAKGGKLSHVQQAYAKSGAVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(2347..3225)
                     /gene="xdhB"
                     /locus_tag="ACHKTW_002953"
     CDS             complement(2347..3225)
                     /gene="xdhB"
                     /locus_tag="ACHKTW_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HFO6463941.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEDPITQRHLPALCAAATSIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHAGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            complement(3236..5533)
                     /gene="xdhA"
                     /locus_tag="ACHKTW_002954"
     CDS             complement(3236..5533)
                     /gene="xdhA"
                     /locus_tag="ACHKTW_002954"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HFO6463942.1"
                     /translation="MEAREATATGESCMRVDAIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIAHGYAVSINDEQARSLPGVLAIFTWEDVPDIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAVAIVVARDELTAEKAAQLVSIEWQELPVITTPEAALAEDAAPIHNGGN
                     LLKQSTMSTGNVQQTIDAADYQVQGHYQTPVIQHCHMESVTSLAWMEDDSRITIVSST
                     QIPHIVRRVVGQALDIPWSCVRVIKPFVGGGFGNKQDVLEEPMAAFLTSKLGGIPVKV
                     SLSREECFLATRTRHAFTIDGQMGVNRDGTLKGYSLDVLSNTGAYASHGHSIASAGGN
                     KVAYLYPRCAYAYSSKTCYTNLPSAGAMRGYGAPQVVFAVESMLDDAATALGIDPVEI
                     RLRNAAREGDANPLTGKRIYSAGLPECLEKGRKIFEWEKRRAECQNQQGNLRRGVGVA
                     CFSYTSNTWPVGVEIAGARLLMNQDGTINVQSGATEIGQGADTVFSQMVAETVGVPVS
                     DVRVISTQDTDVTPFDPGAFASRQSYVAAPALRSAALLLKEKIIAHAAVMLHQSAMNL
                     TLIKGHIVLVERPEEPLMSLKDLAMDAFYHPERGGQLSAESSIKTTTNPPAFGCTFVD
                     LTVDIALCKVTINRILNVHDSGHILNPLLAEGQVHGGMGMGIGWALFEEMIIDAKSGV
                     VRNPNLLDYKMPTMPDLPQLESAFVEINEPQSAYGHKSLGEPPIIPVAAAIRNAVKMA
                     TGVAINTLPLTPKRLYEEFHLAGLI"
     gene            5948..6703
                     /gene="actS"
                     /locus_tag="ACHKTW_002955"
     CDS             5948..6703
                     /gene="actS"
                     /locus_tag="ACHKTW_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase activator ActS"
                     /protein_id="HFO6463943.1"
                     /translation="MSAGRLNKKSLGIVMLLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            6782..6855
                     /locus_tag="ACHKTW_002956"
     tRNA            6782..6855
                     /locus_tag="ACHKTW_002956"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6814..6816,aa:Gly,seq:ccc)
     gene            7020..8234
                     /locus_tag="ACHKTW_002957"
     CDS             7020..8234
                     /locus_tag="ACHKTW_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013814373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO6463944.1"
                     /translation="MLLSDLAIRRAKPKEKAYTLNDGNGLSLLIEPNGSKGWRLRYRF
                     AGKPKMLSLGVYPEVSLSDARTARDEAKRLIAGGVNPSEVRKAQKREQANKCGNTFEA
                     IAREWYEKRLDRWSPSYAEEMIETFEKDVFPYIGGRPIAEIKPMELLAVLSRLNDRGA
                     TEKLRKVRQRCGEVFRYAIITGRAEYNPAPDLASAFAPHKKEHYAYLKVDELPEFFRV
                     FNTYTGSQIVKLGMRLLILTGLRPGELRQAEWQEIDFDNRLWEVPKERMKMRRPHCVP
                     LSEQAIAILEQLKHMTGQYPFIFPGRIQHSKPMSEMAMNVLIRRIGYAGRVTGHGFRH
                     TMSTILHEQGYNSAWIETQLAHVDKNSIRGTYNHAQYLDGRREMLQWYADYMDSLEQG
                     GNVVHGKFGVCA"
     gene            8778..8981
                     /locus_tag="ACHKTW_002958"
     CDS             8778..8981
                     /locus_tag="ACHKTW_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000002831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO6463945.1"
                     /translation="MANNNSFIRLSEVMRRTGYGRAWIYRLISLGRFPKPVKIGSRSV
                     AFIESEVDEWINQRIDASRNNAA"
     gene            10240..10530
                     /locus_tag="ACHKTW_002959"
                     /pseudo
     CDS             10240..10530
                     /locus_tag="ACHKTW_002959"
                     /inference="COORDINATES: protein motif:HMM:NF022029.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ash family protein"
     gene            10573..10755
                     /locus_tag="ACHKTW_002960"
                     /pseudo
     CDS             10573..10755
                     /locus_tag="ACHKTW_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536911.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
     gene            10748..10933
                     /locus_tag="ACHKTW_002961"
     CDS             10748..10933
                     /locus_tag="ACHKTW_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6463946.1"
                     /translation="MLKTFRVFSRAVNARGYTVGISQNVKAENVQAAISTVINKSAKA
                     GLSGVIIHSVHEIKGGQ"
     gene            10933..11136
                     /locus_tag="ACHKTW_002962"
     CDS             10933..11136
                     /locus_tag="ACHKTW_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001186248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6463947.1"
                     /translation="MQEMTLAEASLQAQQVEVISRMIERMIERNHEEIDDHDLSVIAG
                     LIKQLSSNVAVWLMTEEEKRGEQ"
     gene            11194..11493
                     /locus_tag="ACHKTW_002963"
     CDS             11194..11493
                     /locus_tag="ACHKTW_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFO6463948.1"
                     /translation="MASKNTGLTASSKTHPEFMRGDIYRDKYGGIVTIISTRERRITY
                     RREGYDYDCVMPLYQFSRDFSLMQAVPCGKPASNAKAEANIRKMKNMINAFRGKK"
     gene            11490..13628
                     /locus_tag="ACHKTW_002964"
                     /pseudo
     CDS             11490..13628
                     /locus_tag="ACHKTW_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625857.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
     gene            14047..14463
                     /locus_tag="ACHKTW_002965"
     CDS             14047..14463
                     /locus_tag="ACHKTW_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6463949.1"
                     /translation="MIKKTSETESVVRFVSVRGRATLYIPDEHLHHCDEKHIPILIVW
                     KRTVYADVTWLNDSLMLIHRDLFEREEFRRDIEERAEKIYEKYSANSKRSARAIAHHF
                     MTLYDLKAEDAEKAACDLFDMTMGIIQEYRNKERRP"
     gene            14460..14909
                     /locus_tag="ACHKTW_002966"
     CDS             14460..14909
                     /locus_tag="ACHKTW_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFO6463950.1"
                     /translation="MTAQIAAYGRLVDDQQVKQTSKGTPMALARMAVSLPCSQAQDGQ
                     ATLWLSVLAFGKQADALARHEKGDLISVAGNMQISQWTGQNGEIRQGWQVIADSVISA
                     RSARPGGKRGQQGKATEALERAKRQTGQQGNQPPAGGDDPFSDDIPF"
     gene            14926..15342
                     /locus_tag="ACHKTW_002967"
     CDS             14926..15342
                     /locus_tag="ACHKTW_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001076063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ProQ/FINO family protein"
                     /protein_id="HFO6463951.1"
                     /translation="MNQQTTQQDQTTRRPTERPSWRHMTKRQRKNRRRINRLAEYWPE
                     LFGAETIKPLKTGIIRDVLEDAKSRELPIGHGVLRGALTSYIHTSRYLKAIVAGGPRY
                     GLNGQPCGEVTEEDKSVASELLKRRLEQMKQKNSQQ"
     gene            15362..15655
                     /locus_tag="ACHKTW_002968"
     CDS             15362..15655
                     /locus_tag="ACHKTW_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000559728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFO6463952.1"
                     /translation="MITDSVAQKLEERGLWRRAATRWSEVLLHAETDREREEAARRRA
                     ICIIKSRRMPEQFVTFGDVKKAADRTLKEMGINPQDEWRNYSFSDAGDDLALS"
     gene            complement(16102..16488)
                     /locus_tag="ACHKTW_002969"
     CDS             complement(16102..16488)
                     /locus_tag="ACHKTW_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001241336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6463953.1"
                     /translation="MRLTQYKKEILSYFTPDNLAWVTAEIGSPPFDVSGVAYLLYGMD
                     SHNNRYQLESTRRTLEAMVRDGLLERVNVYERRDSVGHGVRRKVARYGLPGRSVVARD
                     GEGRNGAIDGECIRINATGVQLVDGQ"
     gene            16573..16875
                     /locus_tag="ACHKTW_002970"
     CDS             16573..16875
                     /locus_tag="ACHKTW_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFO6463954.1"
                     /translation="MIRDDKAEALENSGLWRRAAERWGELFRQAKTDEERHYITQRRA
                     QCTAKATRPKTTEWGHVCAVRAAATRTLKDMGIDQKKEDPLRGTKYRGETGRRDGS"
     gene            16877..17044
                     /locus_tag="ACHKTW_002971"
     CDS             16877..17044
                     /locus_tag="ACHKTW_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFO6463955.1"
                     /translation="MDTITDKKAEQLESQGLWRRAAARWLDVMKEAHTDPQREHIARR
                     REICLANFRML"
     gene            17613..17774
                     /locus_tag="ACHKTW_002972"
     CDS             17613..17774
                     /locus_tag="ACHKTW_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6463956.1"
                     /translation="MTDRSPTEVIHGIIEEQEERELILMIEEQRERQRKQESTQPDSV
                     SAENQADVP"
     gene            18428..18820
                     /locus_tag="ACHKTW_002973"
     CDS             18428..18820
                     /locus_tag="ACHKTW_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001694413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFO6463957.1"
                     /translation="MRDIQQVLERWGAWAANNRENVTWSSVAAGFKGIIPAKITSRSQ
                     CCDDDAMVICGCIARLYRNNRDLHDLLVDYYVLGKTFMALAHKHECSDSLIGKRLQKA
                     EGVVEGMLMMLDVKLEMDRYVERDSVTA"
     gene            19110..19316
                     /locus_tag="ACHKTW_002974"
     CDS             19110..19316
                     /locus_tag="ACHKTW_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000399369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6463958.1"
                     /translation="MEGVVSHEQAGHRASKNHRFVLFFSQGKNATGINPSSGGISRQH
                     DEQAAQPDTTSRRYYDFRNTFSKA"
     gene            complement(20031..20405)
                     /locus_tag="ACHKTW_002975"
     CDS             complement(20031..20405)
                     /locus_tag="ACHKTW_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6463959.1"
                     /translation="MDTHDKIPPAYSYSPQPVPEAWKMRFSANSAWFFTDDFSVVNAW
                     LKAHSDGVEVVPLFCGSDSDGKRDTAFEEVCAALARMLHMHRLLLEKTRYQEHVTDPE
                     CLRELHEAPIQALNILVKHKLS"
     gene            21155..22765
                     /locus_tag="ACHKTW_002976"
     CDS             21155..22765
                     /locus_tag="ACHKTW_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001613747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HFO6463960.1"
                     /translation="MKGKSALTLLLAGIFSCGTCQATGAEVTSESVFNILNSTGAATD
                     KSYLSLNPDKYPNYRLLIHSAKLQNEIKSHYTKDEIQGLLTLTENTRKLTLTEKPWGT
                     FILASTFEDDKTAAETHYDAVWLRDSLWGYMALVSDQGNSVAAKKVLLTLWDYMSTPD
                     QIKRMQDVISNPKRLDGVPGQMNVVHIRFDSNSPVMADVQEEGKPQLWNHKQNDALGL
                     YLDLLIQAIDTDTINAEDWQKGDRLKSVALLIAYLDKANFYVMEDSGAWEEDARLNTS
                     SVALVTSGLERLSNLLSKKDSVFVSDLLREAKANELDEPLSTTRLNHLIDKGYERITL
                     QLDLGGESPGYLEKDKHYREADAALLNVIYPANLAKINTRRKEQVLKIVKKLAGPYGI
                     KRYEKDNYQSANFWFNDIKTDTDQNSHAKREKSFIPSTEAEWFFDSWYAKSAAIVYKE
                     SRKEEYLNDSVQFMNRSLAQITGENMIGANGRSVPEMALPESYNYIHKSGTLHEAPSP
                     IIPLNWSKASMTLMLKEMSNLINDEGIK"
     gene            22778..22897
                     /locus_tag="ACHKTW_002977"
                     /pseudo
     CDS             22778..22897
                     /locus_tag="ACHKTW_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344669.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion protein"
     gene            22857..23359
                     /locus_tag="ACHKTW_002978"
                     /pseudo
     CDS             22857..23359
                     /locus_tag="ACHKTW_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311753.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YscQ/HrcQ family type III secretion apparatus
                     protein"
BASE COUNT         6465 a         5356 c         5960 g         5578 t
ORIGIN      
        1 accatcaacg tcttcaatga tcgactttta agtaagtctg gaaggtaaag ctatggagct
       61 tgctactacg cagtcagtat tgatgcaaat tcaaccgaca attcagcgtt ttgccagaat
      121 gcttgccagc gttttgcagc ttgaggttga gatcgttgat gaaaacttgt gtcgcgtcgc
      181 cggaactggc gcgtatggga agtttcttgg ccgccagttg agcggcaact cacgcctgct
      241 ccgccacgtc ctggaaacga aaactgaaaa agttgtgaca cagtctcgct tcgatcccct
      301 ttgcgaaggt tgcgatagta aagaaaattg ccgcgaaaaa gcatttctgg gtacgcctgt
      361 cattttacag gatcgttgtg ttggggtgat aagtttgatt gccgttaccc acgagcaaca
      421 agagcatatc agtgataatt tacgcgaatt ttctgattat gttcgccata tatccaccat
      481 ttttgtttcg aaacttctgg aggatcaggg gccaggagat aacatcagta aaatattcgc
      541 gaccatgatc gataatatgg atcagggcgt attagttgtt gatgatgaaa gtcgggttca
      601 gtttgttaat cagactgcct taaaaacact tggtgttgta caaaataata ttattgggaa
      661 acctatccgt ttcagaccat taacatttga gagtaatttt actcatggac atatgcagca
      721 tattgtttcg tgggacgata aaagtgaatt aatcattggt caattgcata acattcaggg
      781 ccgacaatta tttttaatgg catttcacca atcgcatacc agtttttctg tagcaaatgc
      841 acctgatgaa ccacatattg aacaattggt tggcgagtgc cgtgttatgc ggcaattaaa
      901 acgactcatt agccgtattg cacccagccc atccagcgtt atggtggttg gtgaaagcgg
      961 cacgggtaaa gaagtcgtcg cccgagcaat ccataagttg agcggaagac ggaataaacc
     1021 ctttattgct atcaactgtg ccgcgattcc ggagcagctt ctggaaagcg aactgttcgg
     1081 ttatgttaaa ggcgcattta ctggcgcttc tgccaacggt aaaacagggt tgattcaggc
     1141 ggcgaatacg ggcacgctgt ttctcgatga aataggtgat atgccattaa tgttgcaggc
     1201 taaattactg cgcgctattg aggcgcgtga aattctgccg attggtgcca gtagcccaat
     1261 acaagtcgac attcgcatca tttctgcaac taatcagaat ttggcccagt tcattgccga
     1321 aggtaaattc cgcgaagatc tcttctaccg acttaatgtt atcccgataa ctctgccacc
     1381 gctgcgtgaa cgtcaggaag atattgaact attggtgcat tactttttac atctgcatac
     1441 ccgtcgtctg ggatcggttt atcctggcat tgctcccgat gtcgtcgaaa tattgcgtaa
     1501 gcatcgttgg cccggaaacc tgcgcgagtt aagcaatttg atggaatatc tggttaacgt
     1561 agttccttca ggtgaagtta tcgacagcac gctattgccg ccaaatctgc tgaataatgg
     1621 cacaacggag caaagtgatg taacagaggt cagtgaggcg cacctgtcac tcgatgatgc
     1681 gggcggcacg gcgctggagg agatggaaaa gcaaatgatc cgcgaggcgc tttcacgtca
     1741 taacagcaag aagcaagttg ctgatgaact gggcatcggc attgctacgc tctatcgcaa
     1801 gattaagaaa tatgagttgt taaacacata acctcagtga atccggcctg aattcaggcc
     1861 ggatatcctt ttacttcgtt ttctcgcaat ccagaactgt atttacaatc atctgatacc
     1921 ccgtgcagcg acaaagattt cccgccagtc cgcgacgaat ttccgtaatg gttaatggct
     1981 tctcgcgtgg tttcgccagc attgccgtgg tagccataat caggccaggc gtacaaaacc
     2041 cgcactgcac tgcgccggat ttcgcataag cctgctgaac atgagaaagt tttccgcctt
     2101 tcgcttcacc ttccagcgtg cggatctctt ttccttcagc ccaggcggca aggtataagc
     2161 aactgtctat tgctgtgccg tcgaccagca ccgtacaggc accacattca cccacgcagc
     2221 acccttgttt gacacttagc agtccttgtt cgcggagtaa ttccgagagc ggcgtgcctg
     2281 gtgcggcgtg aagctgaaaa ggcatcccgt taatggtgca ttcgatggta attgtttcgc
     2341 tgtgattcat tgcaattttc cccccgccgc ggcgacggct tcgctaatca cttttttggt
     2401 catcgtctgg atgagatgca gacgaaactc tttactggcc cgccatgaag aacgcggggc
     2461 gacatcttgc aggacagatt cgctgatagc ttccagcgtt tgcaggttta atggcgcatt
     2521 ttgtgcagtc tgttcggcat gttggcagcg aatcggcgtt ggcgcggcaa caccaaatgc
     2581 caggcgtaat tcgctgaaat tgccgttatc cagtcggcaa tgtgcggcgc agccaatcgt
     2641 tgaaatatcc attgcgtcgc gcatggcata tttaaaatgc gcgctgcccg cgtgttcttt
     2701 cggctgtggc ggaaaatgaa aggcgacgag gatttcgtca tgctcaagag acactttgcc
     2761 cggcccggtg tgaaagccat taatcgggac gaaacgaaca ccgcgcgggg agtggatctc
     2821 cagtttcgcg tcataaatta gcgttggcgt ggcagaatct gcgctggtgg caccgttgca
     2881 aatatttcca ccgtaggtag cgacgttacg gatctgcggt ccagcaatgg acgtggccgc
     2941 agcacataac gccgggagat gacgttgagt tataggatct tctattagct gggtaaatgt
     3001 cgttgcagag ccgatacgta gcgagccatc ttccgccagc gtaattcccc gcagctccgc
     3061 cagattatga atatcaacaa tatggcgata acggtcattg tgatggtgga gctgaatcag
     3121 tacgtcagtg ccaccggcga gcagtttggc ctgcgggttg tcagccagca ggttgatggc
     3181 atcggcaagg gttgctgcgc gatggtaaga agcaaaatca aacatgatgt tatcctcaaa
     3241 tcaatcctgc cagatggaac tcttcatata accgttttgg cgtcagcggc agtgtattga
     3301 ttgcaacacc ggtagccatc ttcaccgcgt tacgaatagc agcggcaaca ggaattattg
     3361 gtggctcacc cagtgactta tgtccgtatg cggattgcgg ctcattgatt tcgacgaacg
     3421 cgctttccag ttgtggcaga tccggcatgg tcggcatttt gtaatccagc agattggggt
     3481 tacggaccac gccgctttta gcatcgatga tcatctcttc aaatagcgcc cagccaatgc
     3541 ccattcccat tccgccgtgt acctgacctt ctgccagcag tggattaaga atatgccctg
     3601 aatcatgaac gttgaggatg cggttgatgg tgactttgca cagcgcaata tcgaccgtca
     3661 gatcaacaaa ggtacagcca aacgccggtg ggttagtggt ggttttgatg gagctttcag
     3721 cagagagctg cccgccgcgt tcagggtggt agaaagcgtc catcgccaaa tcttttaacg
     3781 acattaacgg ctcttccggt cgttcaacca gcacgatatg gccttttatc agggtcagat
     3841 tcatcgctga ctgatgtagc atgactgcgg cgtgagcgat gattttctct tttaataata
     3901 gtgccgcact gcgcagcgca ggcgcggcaa catagctctg gcgtgaggca aatgcgccgg
     3961 gatcgaacgg cgtaacgtcg gtatcttgag ttgaaataac gcgaacgtcg ctgaccggaa
     4021 cccccacggt ttctgccacc atttgcgaga agacggtgtc ggcaccctga ccgatttccg
     4081 tcgcgccgct ttgcacgttg atggttccat cctgattcat cagaaggcgc gcgcctgcta
     4141 tttctacgcc gacaggccag gtgttagagg tgtagctaaa acaggcgacg ccaacgccgc
     4201 ggcgcaaatt gccttgctgg ttctggcatt ctgcacggcg tttttcccat tcaaagattt
     4261 tccggccttt ttcaagacac tccggcaacc ctgcgctgta aatacgtttg cccgtgagcg
     4321 gattagcatc tccttcgcgg gcggcgttgc gtaaacgaat ttcaacagga tcaataccta
     4381 acgctgtcgc ggcgtcatca agcatagact caacggcaaa tacgacttgt ggcgcgccat
     4441 aaccacgcat cgcaccagcc gaggggaggt tggtatagca ggtctttgaa ctgtaagcgt
     4501 aggcacaacg aggataaagg taagcgactt tattcccccc cgcagaagcg atggagtgcc
     4561 cgtgagatgc ataagcgccg gtgttagaca gaacatccag actataacct ttcaatgttc
     4621 cgtcgcggtt cacgcccatt tgcccgtcaa tggtaaaagc gtggcgggta cgggttgcga
     4681 ggaaacactc ttcacggcta agggaaactt tcaccggaat gccgccaagc ttgctggtca
     4741 ggaatgccgc cattggctct tccagtacat cctgtttatt accaaaaccg ccaccgacaa
     4801 acggtttgat gactcgtacg catgaccagg gaatatccag cgcctgacca accacgcggc
     4861 gaacaatgtg cgggatctgg gtgctggaaa cgatggtaat tcgcgagtca tcctccatcc
     4921 acgccagcga tgttacgctt tccatgtgac aatgttgaat aacgggggtc tgatagtgcc
     4981 cctgtacctg gtagtcggcg gcatcgattg tttgttggac attacccgtc gacatcgtgc
     5041 tttgtttcag taaattgcca ccgttatgga ttggtgcagc gtcttctgcc agcgccgctt
     5101 ctggcgtggt gataacgggt aattcttgcc actcaatgct gaccaattgc gccgcttttt
     5161 ctgccgtgag ttcatcgcgg gccacgacga tggcaacggc gtcaccatga tgacgaacat
     5221 ggcgagttag cagtgcgcga tcggcggtat cgcgcttgtt ttcgtcaagt gtccaggcat
     5281 gcccagcagt agcaaatgga atatcaggca catcttccca ggtaaaaatc gccagtacgc
     5341 ccggcaaact tctggcttgt tcatcattaa tacttacggc ataaccatgt gcgatagggc
     5401 tacgtacata tttcgcataa cacatgcccg ccataacata atcgtcagta tatcgtgccc
     5461 gcccggtgac cttagcaatg gcatcgacgc gcatgcatga ttcacccgta gcggttgctt
     5521 cccgcgcttc cattgaaatc ccctcgattt gaacatttgg ttcttatggc aaagatttag
     5581 tctaagcagg caagatttac gccagaaacg acgataagcg ttaggggagg taggaagtgt
     5641 gatgacgtta aaaaaacaca gatatgatac gaaacaggac tctgaatagg ggcagtgatg
     5701 cgtatcaata tgagtggcga taaaatcaat gtgataaatt gggattatta cgcatcagga
     5761 tgggaaatag ggtaaaaagt caggaagttc gatacctcta cagcgaatat ttagctgatg
     5821 atacttccct ttgccaaatg aaattaacct ctttctctat taccacgttt ttccagaagc
     5881 aagagattgg gtatcaagag gctggctgct atgataaggc gtcttgtttt ttaagcgagg
     5941 aaagattttg agtgcgggac gcctgaataa aaaatctctg ggtatcgtga tgttgttatc
     6001 ggttggactg cttttggcgg gctgttcggg tagcaaatca tccgatacag gaacgtattc
     6061 cggctccgtt tacaccgtga aacgggggga tacgctatat cgtatttcgc gcaccacggg
     6121 aaccagcgta aaagaactgg cgcgactgaa cggcatttcc cccccttaca ccattgaagt
     6181 tggtcagaaa ctaaaactgg gtggggcgaa aagtagcagt agtacacgta aatcaaccgc
     6241 caaatcaacg accaaaaccg catcggttac accgtcatca gcggtaccga aatcttcctg
     6301 gccgccagta gggcaacgtt gttggttatg gccaacgaca gggaaagtta tcatgccgta
     6361 ttcgacagca gatggcggca ataaagggat tgatatctca gctccacggg gtacacctat
     6421 ttacgccgcg ggtgcaggaa aggtggtgta tgtgggcaac cagctgcgtg gctacggtaa
     6481 tctcatcatg attaaacaca gtgaagatta cattacggct tacgcccata atgacacgat
     6541 gctagtaaat aatgggcaaa gcgtgaaggc tgggcaaaaa atcgccacca tggggagcac
     6601 ggatgcggca tctgttcgcc tgcatttcca gattcgttac cgtgcaacgg caattgatcc
     6661 gctacgttac ttgccgccgc agggcagcaa gccaaaatgc tgatggcgaa ttaatcagca
     6721 gtcagcagcg cggcacttgc taaggagagc gtaaggttta taatgcctta cgcatctcga
     6781 agcgggcgta gttcaatggt agaacgagag cttcccaagc tctatacgag ggttcgattc
     6841 ccttcgcccg ctccaaacaa acatctacca acgtctacta aagtctacca atcccccgaa
     6901 tatcaaggcc tccagcgatt ttcctcgtct acaaccgtct acagttatct acctaaatct
     6961 acacacagaa gtgtatagta atgcgtatag gacaaaatcg ttttttgggg gctatacaca
     7021 tgttgctttc agaccttgct atccgtcgcg cgaagccaaa agagaaagcc tatacactca
     7081 atgacggcaa cgggctttcg ttactcattg agccgaacgg ttcgaagggc tggcgattgc
     7141 gatacaggtt tgcaggcaag cccaaaatgt tgtcgctggg tgtttatcct gaggtgtccc
     7201 tttctgatgc caggacggca agggatgaag ccaaaaggct tatcgctggt ggagttaatc
     7261 cgagtgaggt cagaaaggcc caaaagcgag aacaggcgaa caaatgcggc aacacctttg
     7321 aggcgatcgc ccgtgagtgg tatgaaaaac gcctggaccg atggtctccc tcatatgcag
     7381 aggaaatgat agagacgttt gaaaaagacg tttttccgta cattggaggt aggcccatcg
     7441 cagagattaa gccgatggaa ttgctcgcgg ttctatcccg tcttaatgac cgtggcgcaa
     7501 ctgaaaaatt gcgtaaggtt cgccagcgat gtggcgaagt gttccggtac gcaataatta
     7561 ccggacgcgc tgaatataac cccgctcctg atctggcaag cgcatttgca ccacacaaaa
     7621 aagagcatta cgcgtatctg aaagttgatg agctaccgga atttttccgc gtttttaata
     7681 cgtacaccgg aagccagatc gtcaagctgg gtatgcgatt gttgattctt actggacttc
     7741 gcccaggtga attacggcag gcagagtggc aggagattga ttttgataat cgtctgtggg
     7801 aagtgccgaa agaacgaatg aaaatgcgcc gtcctcattg cgtgccactt tcagaacagg
     7861 cgatcgctat tctggaacag ttaaaacaca tgacggggca gtatccgttt attttccctg
     7921 gtcgaataca gcacagtaag ccgatgagtg aaatggctat gaacgtgctt attcgtcgta
     7981 ttggctatgc agggcgcgtt actggtcatg ggttccggca taccatgagt acgattttgc
     8041 atgagcaggg ttataactcc gcgtggatag aaacgcagct ggcgcacgtc gataaaaact
     8101 cgatccgtgg cacatacaat cacgcgcaat acctggacgg acggcgggag atgctccagt
     8161 ggtacgccga ctatatggac tcactggaac agggcggtaa cgtggtgcac ggcaagtttg
     8221 gtgtatgtgc ataaaaacga ctggacaagt atacagatgt atactaacgt acacggaaag
     8281 ctacaggtaa tatccttaca gatggttttt cacgcagtta ccccatcatg tatggacggc
     8341 gctccttgta acaacgaaga tggagtcttc tacgcagctg aaaagctgga tgctctttaa
     8401 cagcctttgg tgctttcctg atgcaggaaa gaatagggat acatgcacct ttacaatgca
     8461 tgtaatgatt caccaatgag gctgcggagc gctgacaaag cgcaattgct ctcttgcctg
     8521 cccaccatgc cctgaaaaag ggcgctcttt aaaactactg gacgtcacgc ctaatcgtgt
     8581 gaccccgccg aaaagcggga atagctctac cttgctggag cataccaacc accaaccaaa
     8641 caatctgaat ttatcttatg caataccaca acatgttttc atgttgtggt ttttgtcgtg
     8701 ttcgtaattt gacgcaaata aaactcttgc taagccacag gaggggattt tcgcgtcaac
     8761 attaaggaga ttgataaatg gctaataaca attcattcat ccgtctttct gaggttatgc
     8821 gccgtactgg ttacggtcgc gcatggattt atcgcctgat cagcctgggg cgtttcccta
     8881 aaccggtaaa aattggttcc cgctccgtgg cttttattga aagtgaagtc gacgaatgga
     8941 ttaaccagcg tattgatgct tctcgcaaca acgccgcgta ataatctttt gggaggggga
     9001 tattctcccc cagcactaaa cctgattcga tagcaggagt tttcaatgaa acgtccgaat
     9061 gtgcccgtac aggggcaggg cttcgttcag cctgaaatca gccagaacga tatattgatt
     9121 cgccacaaag caaagtcgcg tattgattcc cgcctgtttg ctgaacgcat cggcataaag
     9181 cacaaaagcc tttattcgct tattcagaag cacaaaaccg gattgcgtga actgggtatc
     9241 cttccgtttc aaacggagcg attaaaaacc aatcaacacg gagagcgtga acataagttt
     9301 gctctgctga atagcgatca gtttgattac atgcgccata tcgtgagcca tttcgccgct
     9361 gagcgtatga aacacgttaa gcaatactta gaggaggttt tatcaaatta tcgcgctggt
     9421 ggctctgtac ggcgcgaata tccgcatgat gcaggaatgg caggcaaaac aaaggcgacc
     9481 acgaaagggt cgccagtgga gataaaggaa atcaagcgca tcaccaacta tgccatgagt
     9541 gtagctggtg gacaaggcta tcagccaggc ggtcggtttt tagtggcttt taaccgccgt
     9601 gggtatttct cgacaaaatc ttcaagggca aattttttag cttttgtatg tgatgccttt
     9661 gactcgtcat cacatgtttt tccgcgcctt atcctgtttt caataaaacg ggaggcggcc
     9721 tttttattca gcgatgaatc aatggggctg ctccctgttt gctgctcgac aatggcatcc
     9781 atgagtagtt ggtgttgtag ttgtcgtaaa aatttctcat cttctggtgg taaatccata
     9841 cttatttcct gtacagcgga gcgacgcagc acctcacacc acggcgctga tggttgttat
     9901 tcgttttatg gagtcagaga agcaaaagcg gatcgctatc ggtttttagg cgagattaat
     9961 cgccgtgggt atttgtccag aaatgcctca agcgcaaatc gttttggtct ggtgagagga
    10021 gagggattaa gcaaagactc gcgttccagg cgtacttttt cgcgctggcg ctcaagtatg
    10081 gataatgcga tttcctgtaa ttcttcattt gtcattgtct gtacccgtaa agcggtgcgc
    10141 tgtagtacct catatcacag cgcaggtatt ttttatggtt gtaatttgcg tgatgattca
    10201 ggtcgctttg cgacatactg cataaacata aataacgtga tgttattatg tgctttcaaa
    10261 gaaaacgcag gtatttttcg cacaaacttc tctcgcaaca catccgacca cttaagcaaa
    10321 aaagcggcat atactgaggc tatcccttat aaaacgggga tcgggattac caccccgcag
    10381 gagttagagg cgatacacga cgcgccagcg tctttttttg tatcggcgca cacgcacacc
    10441 tcaacaatgg tgggctgtat ggggccgact tcggtcgggc tggtttcctc taacgccagt
    10501 tgtggtaacc ctgtacagtc caccgccagc gagcttacca cctccggcgg tgggtataat
    10561 cccaaagtca gaggagcctt tcacatggct gtatccgcac gcccttactt tgtctggcgc
    10621 tttatgcagt gcctgacagg cagcattgca atattcaccg ttaccgctgc cactgagcgc
    10681 gaagcacgcg cccagctgcc gcacgcacat ctgatttttg tcgcccgtat ccgtcaggag
    10741 gtgactcatg cttaaaacct tccgtgtctt ttcccgtgcc gttaatgcac gcggctatac
    10801 cgttggtatc tcgcagaacg tgaaggccga aaacgtccaa gctgcaatca gcacagtaat
    10861 taacaaatca gctaaagccg gtttatccgg cgtaattatc cactccgttc atgaaattaa
    10921 gggggggcag taatgcagga aatgacactg gcagaagcct cgctgcaagc gcaacaggtc
    10981 gaagtaatca gccgcatgat tgaacgcatg attgaacgca accacgaaga aatagacgac
    11041 catgaccttt ctgttattgc cggacttatc aaacaacttt ccagcaatgt ggcggtgtgg
    11101 ttaatgacgg aggaagaaaa acgcggcgaa caataacggc taaaaataaa cgggaaataa
    11161 agattaagac gactccttca ggagattaat tcaatggcat caaaaaatac tggcttaact
    11221 gccagtagca aaactcaccc cgaattcatg cgcggggata tttatcgcga taagtacggc
    11281 ggcatcgtga cgattatcag cacgagagaa cgacgtatca cctaccgccg tgaagggtat
    11341 gactatgact gcgtgatgcc cctgtatcag ttcagtcgtg atttttccct gatgcaggcc
    11401 gtgccatgtg gtaagccagc cagcaacgca aaagcagagg cgaacatccg aaagatgaaa
    11461 aacatgatta acgcattcag gggcaagaaa tgaaaagcgc accgaattta aaaaaacagc
    11521 cgaaagacaa aatgaccgaa gtcattatct ttgcgggtag tgatgcctgg gcacatgcga
    11581 aagagtggag cgaatgggcc ggaaagcata ttgcagcaga tgatacaccg cccgttattc
    11641 ttggaccgaa acaactggcg agcctggaag acacgaaaat catcgataaa gggcgcaatt
    11701 acgtgcgcgt ttatcgtgct ggtgaaatat cagagacagc acttttacag atagcaacac
    11761 tgcttgcggt ggcgggggta aaggaggcac gttgctacag cggctttgtt gaccagcagc
    11821 cggaggactg gacaccgcgc atggctggac tgaaggatga tgcggaacgc ggcaactgcc
    11881 tggtgatcaa tcttccggca aagacaaatt tcaccggaaa ttatgatgac gaattaaaac
    11941 cccgcgttga atgccgtcct gatggcgttt actgggtgac gccaaaggtt gataagcaat
    12001 ctggcgaaat tatccgtccg ttcgaatggc tgtgttcttc cgttgaatta ctcggttcag
    12061 gtgtcattgg taacgagcat taccgcgtga tgcgctggac agattcgaca acaaatcggc
    12121 ttgtcactat ggctgtgcct tgctgcggaa taggcgacac tgacggctgg cgattactga
    12181 aagccaacgg gcttaaagtc acgacaaacg gaaaacacag gatccccctg gctgactgga
    12241 tgcagttagg cggacagcat gaggaatggc acttaagcac gaaagctggc tggcactttg
    12301 gcgcgtacat catgccggac ggtacggtta tcggcgaatc tgacaagccc gttttgttta
    12361 ccggaaaaag tgccgcgatt aacggttatt ccgtggcagg aacggcggaa agctggcgcg
    12421 acagtgtggc gcggctggct ggtggcaatc cgttcatgat gctgggtata gcgacgtcat
    12481 tatccgcgcc attagtcggg ctgacaggcg ctgatggttt tggcgtgcat tttttcgagc
    12541 aatccacagc aggcaagacc acaacgcaga gcatcgcgtc gagcttgtgg ggcgatcctg
    12601 aggcgctgtg attaacctgg tacggaacgg cgctggggat agctaacgaa gcagaagcac
    12661 acaatcacgg actccttcca ctggacgaga tagggcaggc aggaagcccc cgtgacgtgt
    12721 acgtatcctc atacactttg ttcaatggat tcggaaaatt acagggggca aaggacggcg
    12781 gcaacagaga gctgaaaaac tggcggaccg ttgctatcag caccggagag gtggatatcg
    12841 aaacatacct caaaacgtcg gggataaaag ttaaggtcgg gcaacttgtg cgcctgctca
    12901 atatcccgat tcaaaaagcc acgcagttac acgaatacag caacggaaag gatcacgctg
    12961 acgcattgaa ggcggcatgg caagaaaacc acggcgcagc ggggcgtgag tggattaaat
    13021 ggcttgcgga gaaccagcag gaagctaagg acgcggttag agagagccgt gcacgctggc
    13081 gaaatatcat tccagacaat tacggtgagc aggtccaccg cgtgggtgaa cgtttctcca
    13141 tactggaggc cgcgcttgtg ctttccggtc atgtaactgg ctggtccgtg caggaatgcc
    13201 gcgatgcaat tcagtatgta tttaatgcct gggtaaaaga atttggcacg ggcaacaagg
    13261 aacacaaaca gatcattgag caggcagagg cgtttcttgc tgcctacggc atgagccgtt
    13321 ttgcgcccgt tgattatgac ccagccagcc tgccgatttc tgagctgtac ggctacaggg
    13381 attcgggaag ccgttacgat gaaccattgt tgttttacgt attatcggag ccgtttaaat
    13441 cgaaagtagc ggaaggcttc aacaaggata ccgttgcaaa aatactccac gaagctggaa
    13501 tgctgaaacg accagagagc gggagaggat ggaaaatcaa gaccccacgc cttaagaacc
    13561 ttaatggagc gcaattatgg gcctacggga tgttgttcgc taatcacgat gaggagggaa
    13621 gcgattaact acgcgtatat aagatgaaaa aatgttgcct cagttgcctc aaatagtaat
    13681 aaatatatgt atatgattga tttatatatt ttatttctgt ttttgctgag gcgacaattg
    13741 ctcattttga ggcgacaatt gctcattttg aggcaacaat tgctcatttt gaggcaacaa
    13801 ttacccattt tgaggcaaca cggctgacag gttttgtttg tctgaacgga acgcgttcaa
    13861 cgtgatgttg cctcaaatac cccctattgt tgcctcaaat cgacatgatg ttgcctcaat
    13921 ttttttgtga ttatcaatta aatcaatgcg ttaagacgtt tgaggcaact ggggcgactg
    13981 aggcaacacg tttttcctat atatataaag cgtgtgaatg atgtgatgcc tggaaggaga
    14041 cgattcatga tcaaaaaaac aagtgaaacg gaatctgttg tgaggttcgt atcggtacga
    14101 ggcagagcca cgctttatat acctgacgaa catctgcatc actgcgatga aaaacatatc
    14161 ccgatcctga ttgtctggaa gcgaacggta tacgccgatg tgacatggct gaatgattcg
    14221 ctgatgttga tacaccgtga cctgtttgaa cgtgaagagt tccggcggga tattgaagag
    14281 cgggcagaaa aaatctatga gaaatattca gcaaacagta aaagatccgc acgggcaatc
    14341 gctcaccatt tcatgacgct gtatgacctg aaagcggaag atgcagaaaa agcggcatgt
    14401 gatttattcg atatgacaat ggggattatc caggagtacc ggaacaagga gcgcagacca
    14461 tgacagcaca gattgcagct tacgggcggc tggtggacga ccagcaggta aaacagacca
    14521 gcaaaggaac accaatggca ctggcgcgta tggcggtatc tttgccatgc agtcaggcac
    14581 aggacggaca ggcgacgtta tggctatcag tcctggcatt tggtaagcag gccgacgcgc
    14641 tggcgagaca tgaaaaaggc gaccttatca gcgtggcggg taacatgcag atcagccagt
    14701 ggaccggaca gaacggagaa atacggcagg gctggcaggt tatcgctgac agcgtaatca
    14761 gtgcccgttc ggcgcgtcct ggcgggaagc gggggcaaca ggggaaggcg accgaagcac
    14821 tggaaagagc aaaacgacag accggacagc agggaaatca gccaccagcg ggaggtgatg
    14881 acccgttctc tgacgatatt cctttctgaa tgacaggagc acaacatgaa ccagcagacc
    14941 acacaacagg accagaccac acgcagaccc acggagcgcc catcatggcg acacatgacg
    15001 aagcggcagc gaaaaaaccg caggaggata aaccgactgg ctgagtactg gcctgaactg
    15061 tttggggcag agactataaa gcccctgaaa acgggcataa tacgtgatgt gcttgaggac
    15121 gctaaatcac gggaattacc gattggtcat ggcgtattac gtggcgcatt aacctcatac
    15181 atccacacgt cgcgatacct gaaagccatt gtcgctggtg gccctcggta tggactgaac
    15241 gggcagcctt gcggtgaagt cacggaagaa gataaatcag tcgccagtga actactgaag
    15301 cggagactgg agcagatgaa gcagaaaaac agtcagcagt aatcagccgg aggtaaagcc
    15361 agtgataacg gacagcgtgg cacagaaact tgaggagcgc ggtttatggc gacgtgcggc
    15421 gacccgatgg tctgaggttc ttcttcacgc agaaacagac agggaacgcg aggaagctgc
    15481 cagacgtcgg gcgatatgta tcattaaatc ccgtcgtatg cctgagcagt ttgtcacctt
    15541 tggcgatgtg aaaaaggccg cagacagaac gctgaaagag atggggatta atccacagga
    15601 tgaatggcga aattacagct tcagtgatgc aggcgacgat ttagcgctat cgtgatacgc
    15661 atctcatcgc cgtatttgct caattatcgc gattcgctat aatcaccacc gaaaggccgt
    15721 tgatggtgtt cgcctcgggc aactggagcc cgactgaatg aagccagcca gcggcttttc
    15781 ggcactcatt ccggtgtatc tcccatacat cgggatgagt gaacgacgaa aacatcacgg
    15841 tgatttcgtc atgccgcgat aagaatgcgc ctctttgggc aattgaagcc ctctgtacag
    15901 cgggttatcg cggcaaagtt gtgataaaaa cgcgcctctt tgagcaactg gagctcactg
    15961 taaagcggct tatcacagca ggccgtaacg ggcgctcatc tttgggcaat tggagccctc
    16021 tgtacagaga cccgttgcgg ctgaccccga atgattttgc ccggggcaat tgaagcaccc
    16081 cgcaaagtga ttcggggttt tttattgccc gtccaccagc tgcacacctg tagcattgat
    16141 gcgtatacat tcgccgtcaa ttgcaccgtt ccggccctca ccgtcacgcg caacaacaga
    16201 acgacctggc aggccataac gcgccacctt tcgacgaacg ccatgaccaa cgctgtcacg
    16261 ccgctcatac acattaaccc gctcaagaag accatcacgc accattgctt caagcgtgcg
    16321 ccttgttgat tcgagctgat atcggttgtt gtgtgaatcc atgccataca gcaggtaagc
    16381 cacaccggag acatcaaacg gcggactgcc aatttcggca gtaacccacg ccagattgtc
    16441 aggagtgaag taactgagga tctctttttt gtattgcgtc agtctcatag tgacctttct
    16501 tgttgtagga tgaatccctc atacaataag ccataaggca aattttgaaa ggacagaatc
    16561 ggcggggtac agatgatacg tgatgacaag gctgaagcgc tggagaatag cggcctctgg
    16621 cggcgtgctg ctgaacgctg gggagaattg ttcagacagg caaaaactga tgaagagagg
    16681 cactatatta cccaacgaag ggcgcaatgc acggcaaaag ccaccagacc aaaaacaaca
    16741 gagtgggggc atgtatgcgc cgtcagggca gcagcgacca gaacgcttaa agatatgggg
    16801 attgaccaga aaaaagaaga tcctttacgg ggcacgaagt acaggggtga aacagggcga
    16861 agagacggaa gttaatatgg acacaataac tgataaaaaa gccgaacaac tggaatctca
    16921 gggactatgg cgaagagccg ccgctcgttg gcttgacgta atgaaagagg cccacaccga
    16981 tccgcagcga gagcatatag ccagacggcg ggaaatatgc cttgcgaact tcaggatgct
    17041 gtaaataaga ctgttgacga tatgggaatc agacgggatc ggcgacatta cgggttatgg
    17101 aaataacagc caggtaaatt ggtgaatata cggcaggctg caatcagagc cgaacatagc
    17161 ggcctccgtc accattatca gacagtagcg gtagtccttc catcacagca aaatgctacc
    17221 cacttcacag ccccttagaa gccccttcgg tggggtgaaa aatcccctga aaaatccctg
    17281 aaaaaatgcg caaaaaagtg cgcaaatcga tcataaaacc gatcacaaaa tcgatcacac
    17341 agacaaacag agcaaagcca gatatgacgc ggcttacagg gggatagatc acctcaaaaa
    17401 gtgcgcaaaa cgaaacacaa cagccgcgca aaacgaaacc gatacgtgat gatcgatttt
    17461 tacgccagaa atgaccagag caagggggct gatttccacc cttgtatttt gtgacttact
    17521 taaagtaacc cgcaaaaatg aacgttttta cagggcagaa acgagagtac tggttaacat
    17581 tcctggcatc accgcaactc acagggggac ctatgaccga ccgttcacca acagaggtaa
    17641 ttcacgggat tattgaagag caggaggagc gcgagttaat cctcatgatt gaagagcaga
    17701 gagaacgcca gcgcaaacag gagtccacgc agccggacag cgtgagcgct gaaaatcagg
    17761 ctgatgtgcc gtgatggtgt cgaactgagt aacttaaagt cactctgtga ccagttttaa
    17821 acgagttgca aaaagagccg gagaaaaacc acgaatctgg ggtttttact ggcgcggtgt
    17881 acacggtgga acgcatagcg ttacctgact tattgcattg taaaaagtta gaaaggcacg
    17941 gcctgtaaga tgggatgttg tgagtagttt aaagctaccc gcaaattctt tcgttgtgct
    18001 ggcacaggcg gttaaaccgt cggtaactgg ataatcccca ttgggggaaa gctggctaca
    18061 cccctcacat ctgaggactg ataacgcaaa attgcgttgg ctggcagagt gcaaatttgc
    18121 acgatggcct agcccattga ttctttcgaa aacccccatt gggggagaac gggggaataa
    18181 acctgactca ttgattcttc cacaatcctc aatttgagga agtcggcgcg gtaactcact
    18241 gagattaaag caaagctcaa atctgagatg gctacatccc tcacatctga ggactgttaa
    18301 ccagcaagcc ggaatatatt cacaaactac gaaattttcg tagtttaact ttcatccagt
    18361 acatccccga aaacacgggt atgtacggag ttaccgttac caataattat tcgcgaggtg
    18421 attgttgatg cgtgatattc agcaggttct ggaacgctgg ggagcgtggg cggcaaataa
    18481 tcgcgagaat gtgacctggt catccgttgc cgcaggattt aaggggatta ttccggcaaa
    18541 gataacatca cgctcacagt gctgtgacga tgatgcgatg gtgatatgcg ggtgcatagc
    18601 ccgcctttac cggaacaatc gcgatctgca tgacttgctg gttgattact acgtgctggg
    18661 gaaaacgttt atggcgctgg cacataaaca tgaatgctct gattcactca tcggaaagcg
    18721 gctacagaaa gcggaaggtg ttgttgaagg catgctgatg atgctggatg tgaagctgga
    18781 gatggatcgg tatgttgagc gcgattcagt gacggcataa cgcgcataaa aaaaccgcag
    18841 ccatgaggaa actgcggtgg agaaatacgg cttgccaatc aatcatgaaa aacgtaatcg
    18901 cctgataatt atgccacgag acaaaggacg gtgcaccctg aatggtcata atgaccactc
    18961 agaccatcaa ggcaaccata acgaacatcg ttacagttgg agcttttacc ccgttggggg
    19021 aaaaggcgca tacctgaatc atcattatgg tgatcaaggg tatgtaaagc ccaccagcct
    19081 gattaaaggt taaccggaaa aagccaggta tggagggagt ggttagccac gaacaagccg
    19141 gacaccgcgc cagcaaaaac cacagattcg tgcttttctt cagccagggt aagaacgcca
    19201 cggggattaa tccgtcgtct ggtggaatca gtcggcagca tgacgaacag gccgcgcagc
    19261 cggacaccac cagcagaagg tattatgatt ttcgtaatac ctttagcaaa gcgtgaatca
    19321 tcattaccgt agggatggtc ataatgacca caccttcccc caccggaaca aggaaggcag
    19381 accaacgaag atttcgtaag tcctgacccg cacaaggtat taaataatca ctacaagcca
    19441 ttgattaaag ttaattataa cgattcgtta gaatcaggct tttcagtctg gttaacggtt
    19501 gaccggaaaa aatctggtag ggtatccgcg atgttgggtt tattcatcgc ctgatgttgc
    19561 ctcaaatccc cctattgttg cctcaaatca gcatgatgtt gcctcaattt ttaagacgat
    19621 attcaattaa atcaatgcgt taattgattt gaggcaactg aggcgactga ggcgacacaa
    19681 tttcgctata tatataaagc gagtgagtga tctgaatggt catgatgacc acaccccacc
    19741 ggaacaagga cagaagaatt acgaaaattt cgtaaaatcc tccccgcatc ccccacggtt
    19801 gcccgttaag tgcaagcgca caggcacgac ctcgttaacc agttaccagc gctcacgctc
    19861 aaatatccac aggaatgggg ataaatcgta ttctggcgca atgttcactg tcggggcgag
    19921 tattcaccgg aaaacgtcat acaggcttac agtcggctta aaattagatg gacggatgac
    19981 agggcgggat ggtgagacct acacaaaaag tgtaggtctc catgacacgg tcaggacagt
    20041 ttgtgtttaa ccagaatatt tagcgcctgt atcggggctt cgtggagttc ccgcagacat
    20101 tcagggtcag taacatgttc ctggtatctt gttttctcaa gtaacaagcg gtgcatatgc
    20161 agcatacggg caagagccgc gcacacctcc tcaaatgcgg tatcccgctt accgtcactg
    20221 tcagagccac aaaacagcgg tacaacctcc acgccgtcgc tgtgggcctt tagccaggca
    20281 ttaaccacgc tgaaatcatc ggtgaagaac cacgcactgt tagcgctgaa tcgcattttc
    20341 caggcttccg gtactggttg cggggagtat gaatatgctg gtggtatctt gtcgtgagta
    20401 tccataggct atcgttccgt cattctgtta ttcaccacag cggtaaaaac gccatgaagg
    20461 accagaatac cgattgcctc gtttgctgta tgaggtgctt accacacatt aggtacacag
    20521 atgaatcagg tggaagtgtg attaccggaa aaaatccctt tcagtttgac gaaaaaaaac
    20581 cgcagtgtga taacactacg gcacgccacc aaagaaacat aacaaggaat caacaatgag
    20641 taacacctgt aagccttcgg ggtgattgta acacatgagc aatcttttac tggaaactgt
    20701 ttttcactgg atcacgtatc gttatgggga ttcccatatc ggagagcatc caccagcttc
    20761 ataggggata accggaaaaa ttccggtaaa tatttgcctg agtaagtcat caagcgcctg
    20821 ctaaatgtgt atagaaatgt gtataggatt gattttataa catgtggaaa tggctttatt
    20881 actggttgca tggcttgttg ttctattccc ttcgccgctc caatttatct tcacctgatc
    20941 aatctgccat ttttgctgta tctgattttc tatattatct taattcatcg cttcagactt
    21001 ttcgcaatcg ccgtaatact tccccatttt gttgtaaata aaaagcgttt acgcaatgca
    21061 atccttttgt aacaacccag gtaaaacacc ttattttcaa gttgttaaat aagtggttat
    21121 ctacgctttt aacaaacgat tgatggaata ttttatgaaa gggaaatctg cattaactct
    21181 tctgctcgct ggtatatttt cgtgcggcac atgccaggcg acaggggcag aagtgacgag
    21241 tgaatctgta tttaacattc ttaattccac gggggcagca acagataaaa gttatttatc
    21301 gttaaatcca gataagtatc ctaattatcg tcttttgatt cactcagcca aattacaaaa
    21361 tgaaataaag agccactata ccaaagacga aattcaagga ttgctaacat taacagaaaa
    21421 cacacgaaag ttaacgttga cggagaaacc ctggggaacg tttattcttg cttctacttt
    21481 cgaagatgac aaaacagcgg cagaaaccca ctatgatgct gtctggctga gggatagttt
    21541 atggggctat atggcattag tatcagatca gggaaatagt gtagccgcaa aaaaggttct
    21601 gctcaccctg tgggattata tgtctacgcc cgaccagatt aagcggatgc aggacgtcat
    21661 tagcaacccc aaacgactgg atggagttcc cgggcaaatg aatgttgtgc atatccgatt
    21721 tgacagcaac tcccccgtga tggcagatgt gcaggaagaa ggcaagccgc agctgtggaa
    21781 ccataaacaa aatgatgcgt taggtcttta tcttgatctc cttatacaag caatcgacac
    21841 tgatacaatt aacgctgaag attggcaaaa aggtgaccga ttaaaatcgg ttgcgctgct
    21901 gattgcttat ctggataaag ccaatttcta tgttatggaa gattccggag catgggaaga
    21961 agatgcccga ttaaacacat cttccgttgc actggtgacc tctggactgg aacgtttgtc
    22021 gaacttactc tcgaagaaag actctgtttt tgtctctgat ttactacgcg aagctaaagc
    22081 caatgaactg gatgaacctc tttcaacaac gaggttgaac cacctcatag ataaaggtta
    22141 tgaaagaata acgttacagc ttgatttagg gggagaatcc ccaggttatc tggaaaaaga
    22201 taaacattac agagaagccg atgcggcttt acttaatgtt atttatcctg caaatcttgc
    22261 aaagataaac accagaagaa aagagcaggt tctaaaaata gttaaaaaac tagcaggtcc
    22321 gtatggtata aaaagatacg agaaggataa ttatcagtcg gcaaattttt ggttcaatga
    22381 tattaaaacc gataccgatc aaaattcaca tgcgaaaaga gaaaagagct tcattccatc
    22441 aacagaagca gaatggtttt ttgactcatg gtatgcaaaa tcagcagcaa ttgtttataa
    22501 agaatcaaga aaggaagagt atttgaatga ctcagtgcaa tttatgaata gatcattggc
    22561 ccaaataaca ggcgagaata tgattggtgc aaatggcagg agtgtacctg aaatggcact
    22621 tcccgaaagc tataactata tccacaagtc gggaactttg catgaagcgc caagccctat
    22681 cattccgcta aattggtcga aagcttcaat gactctcatg ttgaaagaga tgtccaacct
    22741 tatcaatgat gagggaatta aatagttttc ataatgagtt acagcacaac gttgggtatt
    22801 gactgagcaa gatgaacgac aaggacaacg acatcagcct tatgaggagc aagaaaatga
    22861 aagcaaattc gaaaacgatc agaaggatga aagttaatgt tcggtctgag gaaagtaaat
    22921 cgaaacactc atacgtttga aaccactttt caaaattgga aagaaaatgg cgaagatgtt
    22981 gcattattaa tgcccgaatt tagtgctaaa tggttaccga tagcagaaga gagtggcagc
    23041 tggtctggat gggtattgct tggagaaata tttcccttaa tttcatctga gctcgctggg
    23101 atggctttaa tgccagaaac ggaaaggtta ataggggaat ggctcagttt atccagttcc
    23161 ccgttaaatc taaagcatcc tgaactaaaa tataatcgtt tgcacgtagg taaagtattt
    23221 gatggagtat tgagccctgc tcaaccacta ataagaatat gaacagggga attgaatctt
    23281 tggttagata aagtcacagt ctgccaatac ggaaacgctc cagtgttaga caaaaaatca
    23341 ttatattggt ccattcatt
//
LOCUS       DAZFQD010000076        23205 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.076, whole genome shotgun sequence.
ACCESSION   DAZFQD010000076 DAZFQD010000000
VERSION     DAZFQD010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23205
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.076"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(268..885)
                     /gene="tdk"
                     /locus_tag="ACHKTW_002979"
     CDS             complement(268..885)
                     /gene="tdk"
                     /locus_tag="ACHKTW_002979"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFO6463961.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
     gene            1490..1903
                     /gene="hns"
                     /locus_tag="ACHKTW_002980"
     CDS             1490..1903
                     /gene="hns"
                     /locus_tag="ACHKTW_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0071824 - protein-DNA complex organization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histone-like nucleoid-structuring protein H-NS"
                     /protein_id="HFO6463962.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            complement(2047..2955)
                     /gene="galU"
                     /locus_tag="ACHKTW_002981"
     CDS             complement(2047..2955)
                     /gene="galU"
                     /locus_tag="ACHKTW_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFO6463963.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(3157..4170)
                     /gene="rssB"
                     /locus_tag="ACHKTW_002982"
     CDS             complement(3157..4170)
                     /gene="rssB"
                     /locus_tag="ACHKTW_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HFO6463964.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(4262..5167)
                     /gene="rssA"
                     /locus_tag="ACHKTW_002983"
     CDS             complement(4262..5167)
                     /gene="rssA"
                     /locus_tag="ACHKTW_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004622 - lysophospholipase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HFO6463965.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVERKMDELLPLVRTNI"
     gene            5280..5738
                     /locus_tag="ACHKTW_002984"
     CDS             5280..5738
                     /locus_tag="ACHKTW_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HFO6463966.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELIAGFAHTEWLGLTVFENCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            5788..6630
                     /gene="purU"
                     /locus_tag="ACHKTW_002985"
     CDS             5788..6630
                     /gene="purU"
                     /locus_tag="ACHKTW_002985"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HFO6463967.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            6790..6874
                     /locus_tag="ACHKTW_002986"
     tRNA            6790..6874
                     /locus_tag="ACHKTW_002986"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6824..6826,aa:Tyr,seq:gta)
     gene            6909..6993
                     /locus_tag="ACHKTW_002987"
     tRNA            6909..6993
                     /locus_tag="ACHKTW_002987"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6943..6945,aa:Tyr,seq:gta)
     gene            complement(7177..7854)
                     /gene="narI"
                     /locus_tag="ACHKTW_002988"
     CDS             complement(7177..7854)
                     /gene="narI"
                     /locus_tag="ACHKTW_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HFO6463968.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRLFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(7854..8564)
                     /gene="narJ"
                     /locus_tag="ACHKTW_002989"
     CDS             complement(7854..8564)
                     /gene="narJ"
                     /locus_tag="ACHKTW_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HFO6463969.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLSKEDAHALG
                     IFLRDLTAMDPLDAQAQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLSQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVMFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(8561..10099)
                     /gene="narH"
                     /locus_tag="ACHKTW_002990"
     CDS             complement(8561..10099)
                     /gene="narH"
                     /locus_tag="ACHKTW_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HFO6463970.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLEVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(10096..13839)
                     /locus_tag="ACHKTW_002991"
     CDS             complement(10096..13839)
                     /locus_tag="ACHKTW_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HFO6463971.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVESLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGHLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVMGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
     gene            complement(14232..15623)
                     /gene="narK"
                     /locus_tag="ACHKTW_002992"
     CDS             complement(14232..15623)
                     /gene="narK"
                     /locus_tag="ACHKTW_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HFO6463972.1"
                     /translation="MSHSSAPERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATDTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWTVYGRHSKK"
     gene            15962..17758
                     /gene="narX"
                     /locus_tag="ACHKTW_002993"
     CDS             15962..17758
                     /gene="narX"
                     /locus_tag="ACHKTW_002993"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HFO6463973.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSQAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            17751..18401
                     /gene="narL"
                     /locus_tag="ACHKTW_002994"
     CDS             17751..18401
                     /gene="narL"
                     /locus_tag="ACHKTW_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HFO6463974.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(18402..19796)
                     /gene="ychO"
                     /locus_tag="ACHKTW_002995"
                     /gene_synonym="iatA"
     CDS             complement(18402..19796)
                     /gene="ychO"
                     /locus_tag="ACHKTW_002995"
                     /gene_synonym="iatA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HFO6463975.1"
                     /translation="MSRFVPRIIPFYLLLLVAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDDGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLLDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSVEQYFGDRVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            19981..20334
                     /gene="ychN"
                     /locus_tag="ACHKTW_002996"
     CDS             19981..20334
                     /gene="ychN"
                     /locus_tag="ACHKTW_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HFO6463976.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            complement(20378..21073)
                     /gene="chaC"
                     /locus_tag="ACHKTW_002997"
     CDS             complement(20378..21073)
                     /gene="chaC"
                     /locus_tag="ACHKTW_002997"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HFO6463977.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     GPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGCTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(21231..21461)
                     /gene="chaB"
                     /locus_tag="ACHKTW_002998"
     CDS             complement(21231..21461)
                     /gene="chaB"
                     /locus_tag="ACHKTW_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HFO6463978.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKDDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            21731..22831
                     /gene="chaA"
                     /locus_tag="ACHKTW_002999"
     CDS             21731..22831
                     /gene="chaA"
                     /locus_tag="ACHKTW_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HFO6463979.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLALVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQTKTHQSLFVYEHEDDSDDDDPHHGKPSAH
                     SSVWHAIWLIIHLIAVIAVTKMNASPLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
BASE COUNT         5819 a         6064 c         5582 g         5740 t
ORIGIN      
        1 tctggggccg tttttttgtt tacccaaagc aacttttcca taaaccgaca gcattagcct
       61 tcatcatatt tgcgacgatg tataacgcct aaacacaggg atattgtact ttacaggtca
      121 caagtcaacg tcggtgctta agagccctgt gaggcgtata gcggcgttaa aaaactgccg
      181 agaagggtat atagcccgga agaagtgcgt aaaacgaact gacaggataa aagtgccctg
      241 ctcaccctgt cagtaaagaa attcttatta atcgtggcga tgcctttcct gaatagccgt
      301 taatgagccg acttgtaacg cctctttata gtgtttacgg catacagaaa cgtatcgttc
      361 attaccacca attaccacct gctcaccttc gttataaggt ctgcctgctt gatcaagacg
      421 cagcaccatg cttgctttac ggccacaaaa acagatggtt tttaattcaa ccagtttgtc
      481 ggaccatgcc agtaagtatt ggctgccaat aaataattca cctcgaaaat cggtacgtaa
      541 accataacaa agtacgggta tatcgagttg atcgacaacc tccgataatt catatacttg
      601 ttgtctggtt aaaaactggc attcatcaac cagtacgcaa tgaattgcct gctgttcatg
      661 ttccgcacga atctcatcaa ataatgatga attttggtta aataattttg caggcgatga
      721 caaacctata cgcgaactga ctttcccggc accaaagcga tcatcaattt ctgccgtata
      781 tacgacagtg cgcatgccgc gttcctggta attgtatgaa gattgcaaca atgctgtaga
      841 cttacccgca ttcattgcgg aatagtagaa atatagctgt gccatcagcc acaggccctc
      901 aatgatatga aataaagttg gctggagttt atcataattc gtcagttttt cagctcaaca
      961 gtatgcacag aagtgggtat atgcgttctc ccttacgaag ccttgcataa tccttctgag
     1021 ctatcattac aactgcctcg cttgttataa gcgggtaaat gactgctggt aactattcac
     1081 aatctttaac ctgttgcgca agtaatagcc ctctgttgac ctccaggaga tagtgcaata
     1141 ctaagtccat gctcttattg cgactgttct acttttcatc attcgcttaa tagggaattc
     1201 tcgtaaacac aactaataca gaagactgaa aggtcgtcag cctacgataa tctccccata
     1261 aaatgtgaca tgaatcagga agttttaacc tcacgtgctg cgaaatcatc ggtgtaaata
     1321 gggctatatg ccgcgtcttt tctggctaat tttatgaaaa gatatttatt ggcggcacaa
     1381 aataaagaac aattttgaat tccttacatt cctggctatt gcacaactga atttaaggct
     1441 ctattattac ctcaacaaac caccccaata taagtttgag attactacaa tgagcgaagc
     1501 acttaaaatt ctgaacaaca tccgtactct tcgtgcgcag gcaagagaat gtacacttga
     1561 aacgctggaa gaaatgctgg aaaaattaga agttgttgtt aacgaacgtc gcgaagaaga
     1621 aagcgcggct gctgctgaag ttgaagagcg cactcgtaag ctgcagcaat atcgcgaaat
     1681 gctgatcgct gacggtattg acccgaacga actgctgaat agccttgctg ccgttaaatc
     1741 tggcaccaaa gctaaacgtg ctcagcgtcc ggcaaaatat agctacgttg acgaaaacgg
     1801 cgaaactaaa acctggactg gccaaggccg tactccagct gtaatcaaaa aagcaatgga
     1861 tgagcaaggt aaatccctcg acgatttcct gatcaagcaa taatcttttg tagattgcac
     1921 ttgcttaaaa tcccgccagc ggcgggattt tttattgtcc ggtttaagac aatttaataa
     1981 gaataagcta taaaaaaacg gcgtcgattg ctcaacgccg tttcgtggat aacaccgata
     2041 cggatgttac ttcttaatgc ccatctcttc ttcaagccag gctttaaatt ccgtgccaag
     2101 ggtgttatga cgaataccgt attcaacgaa ggcctgcatg taacctaatt tattaccgca
     2161 gtcatggctc ttccctttca tatgataggc ttccaccgtt tctttttcga tcagcatatc
     2221 aattgcgtcg gtgagctgaa tttcatcacc agctcccgga ggggtttttg ccagcaacgg
     2281 ccaaatatcc gcgctaagta cgtaacgacc cacaatagcg agattagacg gcgcaacatc
     2341 cgctttcggt ttttctacca caccaaccat cggtacgctt tcaccaggcg ctaattcaac
     2401 gcctttgcaa tccacaacgc catatgcggt cacatcagca accggttcaa ccatgatctg
     2461 gctatgaccc gtttcatcaa agcggcggat catctctgcc aggttatcct gtgacaaatc
     2521 ggattcatat tcatccagaa taacatcagg caaaataaca gctaccggtt catcacccac
     2581 taccgggtga gcacacaata ccgcgtgtcc caggcctttc gccagaccct gacgaacttg
     2641 cataatagtc acgtgcggtg gacaaataga ctgcacttca tcaagcagtt gacgttttac
     2701 acgtttttcc agcattgctt ccagttcaaa actggtatca aagtggtttt caatagagtt
     2761 tttagatgag tgtgtaacca gcacaatttc agtaatgcca gccgcaatac attcattcac
     2821 gacgtattga attaatggct tatcgacaag tggcagcatc tctttcggga tggctttcgt
     2881 cgccggcaac atcctggttc ctaatcccgc aacggggata acggcttttt tgactttcgt
     2941 attaatggca gccatttaaa ttctcctgga ctgttcgtgt tttgaacgtg ttcatatttc
     3001 tgtatcgcat cccagtatat cagcaccccg aaggggctaa gttctgaaaa ggaggcgagt
     3061 tagcataatt aagacaatta cttatacgaa tggcgacgat agtaccacca gccgacatta
     3121 gcaggtaatg caaatttagc ccgcgttatc gtttgctcat tctgcagaca acatcaagcg
     3181 cagtcgacca ccggttcccc atatttggca ttgccaggca tcgcatcgct ggctcaattg
     3241 attcaaataa gcgttaccta aagtgcctaa cggaacacca ttactgattt gcacctggtg
     3301 ttcgccggta tttaacgtcg cattcagacc cgcagaaacc agaatgagat ttttcagttc
     3361 gcgatgataa tagccaacta atagcggaaa ctgccccggc agattggcct gacgaagtaa
     3421 atggtttacc tgcttcaata acgcgcccaa ctctggcaac cgttgatttt ggtgtgcaag
     3481 ctgttcctgt aataatccgt taaacaatgc gcgtaataac aaggcagcaa gtacgccatt
     3541 atgtccagct cgggtgacat caaggcaata aaatgccaga tcgttttccg aaagtgcggc
     3601 aatatcaagc accaggccgg gtttgtccgc ggcaaccaat tgacgataat taacccggca
     3661 atgggaaatc acctgctgaa ccggcggttg tagttcctgt aataatttcg ccgctgcggc
     3721 agggttatca accattgcat cccagtcgcg aaaaagcctt tcctcttcct caacgcgcga
     3781 attaaacatg ctgggataga gacaggcaaa aaccatctcg cgcaagcgat tcagatcttt
     3841 aactggtttc agcaaaacat cttcaacgcc cagacgtaac gctttggcaa tatctgccat
     3901 attttcagtg gcagatatca ccagaaccgg ggtctggtcg cctctgttac gtatatgctc
     3961 cagcagttta agcccgttca ttcgtggcat cgcgatatca catatcatca ggtctggagt
     4021 gaaacctccc agcaactcaa gggcatccac cccatcagcc gccagtaccg ttgtcgctcc
     4081 caatgaggaa aaccatgaat ccagaagcga gcgaaatacc tgctcatctt caacaatgag
     4141 aatctgtttt ccgaccaatg gctgcgtcat gttctctccc ctgactggct ttactcaata
     4201 gtggcatgct attgccacct gcgcctgtca gaattaactt aagtgtagat aaaaattctg
     4261 gtcaaatgtt ggtgcgtacc aacggcaaaa gttcgtccat tttcctttcc actgccagct
     4321 gtccggccgc aatggcagcg tgcgcgcgat ggaaatcaag cgtagatatt tgcgggcaaa
     4381 caggttgaat cagaatatcg ggcggatcac ctgccatgcg gttcctttta aggcggttct
     4441 ccagcacctg gatagaagtg gtcataatct ctgttgccgt tggtgctgtc accgcccgac
     4501 gtgtcgttat gctgcctaac ctttctttca gacgcgcatg ccacggcaga gaatcaccat
     4561 tctcgctatt ttcttcactg acattaaagg agagcaaatc ttgttgcatt aaatgagcat
     4621 cgtgctgcag gtcgaccgct atcacaatat cagcacccaa tgcacgcgtg agcgaaatag
     4681 gaattgggtt aacgactgcc ccatcaacca gccagtagcc gttatgcgcg actggtgcca
     4741 tgagtcctgg aatactgcat gacgcgcgaa tagcaagatg gagatcgcct tcagtaaacc
     4801 ataattcacg cccagtactt aaattggtgg caacagccgc gaagcgacgg gaacaatttt
     4861 cgatctctgt ttccggcatt atctcgcggt attgattgaa gacacgctcg ccgcgcagta
     4921 acccaccgcg ctgccaggag agatccatca ggcgtaaaac atcccaataa ctgaaagagg
     4981 ttacccaatc ttccagcgca gataatcgat cgcatgcata ggccgcgccc accagcgaac
     5041 caattgaaca tcctgcaacg atatcaattt caatacccac tttttttagc gcattaataa
     5101 cgccaatatg cgaccaacct ctcgccgcgc cagatcccag cgccagccct atttttatct
     5161 ttctcattat tcccgcaaga ttcccctgga atgcaatcgt agccacatcg ctactgctcg
     5221 gttaacatag tgctactcag gcggttaata cgccgttatt gtttccaggg agattatttg
     5281 tgtctcagct ttgtccctgt ggtagtgctg tcgagtatag cctatgttgc cacccttatg
     5341 tgtctggtga aaaggttgca cctgatccag aacatctcat gcgttcgcgt tactgcgctt
     5401 ttgtgatgca agacgcagat tatttaataa agacctggca tccatcttgt ggagcagcgg
     5461 cgttacgtgc cgaattgatt gccggatttg cgcatactga atggttggga ttgaccgtat
     5521 ttgaaaattg ttggcaggac gccgacaata tcggatttgt cagttttgtt gctcgattta
     5581 ccgaaggtgg taaaacaggt gcaataattg aacgttcgcg atttttgaaa gaaaacggtc
     5641 aatggtacta tattgacggt acacgtccgc agtttggtcg caacgatccc tgcccttgtg
     5701 gttcaggtaa aaaatttaaa aagtgctgcg gccaataatg gttgacggta cggtttagca
     5761 aacactctca acaaggtttt tccagcaatg cattcactcc aacgtaaagt tctgcgtact
     5821 atttgtccgg accaaaaagg tctgatcgca cgcattacca atatttgcta caagcacgag
     5881 ttaaatatcg tacagaacaa tgaatttgtt gatcaccgta ccgggcgctt ttttatgcgc
     5941 acggaactgg aagggatttt taatgattcc acccttctgg cggatctcga tagcgcattg
     6001 ccagaaggct ccgtgcgtga gctgaatcct gccggtcgtc gccggatagt gattctggtc
     6061 actaaagaag cgcattgcct tggcgatttg ttgatgaaag ccaattacgg cggcctggat
     6121 gtcgaaatcg cggcagttat tggtaaccac gatactttac gttctctggt tgagcgtttt
     6181 gatattccgt ttgagctggt aagccatgaa gggttaagcc gcaacgagca cgatcaaaag
     6241 atggcggacg ccattgatgc ttatcaacct gactatgtgg tgctggcgaa gtatatgcgg
     6301 gtattaacgc cggaatttgt ggcacgcttc ccgaataaaa tcatcaatat tcaccattca
     6361 ttcctgcctg cgtttatcgg cgcacgtcct tatcaccagg cctatgaacg tggtgtgaag
     6421 attattggcg caaccgctca ctatgtgaat gacaatctgg acgaaggccc aatcatcatg
     6481 caggacgtta ttcatgtcga tcatacctac acagctgaag atatgatgcg cgcaggtcgt
     6541 gacgtcgaga aaaacgtctt aagtcgtgcg ctatacaaag tactggcaca gcgcgtcttt
     6601 gtttacggta atcggacgat tattctttaa tcgccagcaa aaataactgg ttacctttaa
     6661 tccgttacgg atgaaaatta cgcaaccagt tcatttttct caacgtaaca ctttacagcg
     6721 gcgcgtcatt tgatatgatg cgccccgctt cccgataagg gagcaggcca gtaaaaagca
     6781 ttaccccgtg gtggggttcc cgagcggcca aagggagcag actgtaaatc tgccgtcatc
     6841 gacttcgaag gttcgaatcc ttcccccacc accatctcaa gtattacctc aaaatttgaa
     6901 ttacccctgg tggggttccc gagcggccaa agggagcaga ctgtaaatct gccgtcatcg
     6961 acttcgaagg ttcgaatcct tcccccacca ccatcgcttc cttaaataat caaaacctgt
     7021 tactccgagc taattttgcc cgatggcacc acgtttaccg ggcctacaat atcctcccct
     7081 gtaggtcgga taagcgaagc gcatccgaca actcaccccg caccgtgccc ataaaaaaac
     7141 cccgccgaag cagggtctat gtgaactaaa attcgcttag tgacgagcgc gcaccagctg
     7201 gtacttacgt gtcagatact ccaccggtac gctccagatg tgtaccagac gcgagaacgg
     7261 gaacagcagg aataacgtca tccccagcac caggtgcaga cggaagataa acgccacacc
     7321 atcgaggtgt tgagaagcgc caccgtggaa ggtcaccacc gactgcgccc agccaaccag
     7381 tttcatcatc tcgctaccgt ccatatgctg agcggagaac ggaatggtca acaggcccag
     7441 cgcgcactgg ataacgagca gcgacaggat caggatatcc gctccggtag tggttgcacg
     7501 cacgcgtggg ctgaacagac gacgcttcag caacagcacg ccgccaatca gacacagcac
     7561 gccgctggca ccaccagcaa acatcgccat tttctgtttc acttcaatcg gcagccaggc
     7621 ttcatacatc cagtgcggcg tcagcatacc gaagaagtga ccaacaaaaa tccccagaat
     7681 accgatatgg aacaggttcg acgccaggtt catccctttg cgatccagca tctggctgga
     7741 cgccgcgcgc caggtgtact gcccgtagtc ataacgcagc cagctaccaa tcaggaagac
     7801 cgccccggct atgtacgggt agatatcaaa gaagaacata ttcaggaatt gcattagtgc
     7861 tgtcctccgg tggtgatatt cagatattgc ggcgcgacgg caccggcaaa gcgacgctga
     7921 tgagcagtga ttgctgaatc accgcagcct ttgtcagcaa agaatttaac ctgctcttct
     7981 tcccagacag catccagcgc ctgcggcgta tcatcgcgcg cttcgtcggc aattttttcc
     8041 gccactttgt cgctgtcgat agcggtattg gccaatttca gcagcagatc aaacatcacg
     8101 gcataacggc tttcacgctg ttgcagacgc gcgctcagca atgccagaat cggcgcgata
     8161 tctttcaaac cttccacggc ttcgctttgc ggcagctgcg acaggtactc cagatacagc
     8221 ggcagatggt ccggtaattc gcggctgttt aactgcaagc cgtgctgctc gtactgcgcc
     8281 agcaggtcca ccatcgcctg gccgcggtca cgggattcac cgtgaacatg ttcaaatagc
     8341 aacagtgacg tggcgcggcc acggtcgaac agttcgctgt actgggcctg ggcatcgagc
     8401 ggatccatcg ccgttaaatc gcgcaggaaa atgcccagcg catgggcatc ttctttcgac
     8461 agatttttcg acgcagcaat cgcctcaaac atctcttgtt gatgctgcca taaggcagca
     8521 tccggatatt caaggagacg cgatacaatc acgagttcga tcatggatgc ggctccgttt
     8581 tgctggtcac atcgatggca tcgatacgac ggctgttgaa cagattgaat ttggtatctg
     8641 aaccgtggca gccatcacca aaggtaaagc cgcagccatt tttctccggg aaggcttccc
     8701 gtgccagttc acgatgacta ctcggcacca caaagcgatc ttcgtagtta gcaatcgcca
     8761 gataacggta catctcctgt gcctgggctt cggtcagacc gacctcttcc agcgcacggg
     8821 tatcaacttt accgtcaacg gtttcagcac gtttgtaatg acgcatcgcc agcatacgtt
     8881 tcagtgcgcg cagtaccggt ttggtatcac cggcggtcag cagattcgcc agatactgta
     8941 ccggaatacg caagctttcg acgtctggca gaatgccgtt gctacccagc tcacccgcgt
     9001 ctgctgcaga ctgaatcgga gacagaggcg gcacgtacca gaccatcggc agtgtgcgat
     9061 attctggatg cagcggcagc gccagtttcc attccattgc cattttataa accggcgact
     9121 gctgtgcggc ttcaataacg ctcagcggaa taccgtcttt aatcgcctgc tcgatgactt
     9181 tcggatcgtt cggatcgagg aacacctcca gctgacgctg gtaaagatct ttctcgttct
     9241 cggtgctggc tgcacgttca atcgcgtcgg catcgtacag cagcacgcca agataacgga
     9301 tacgaccgac acaggtttct gagcacacgg tcggctgacc cgcttcaata cgcggatagc
     9361 agaagatgca cttctcagac ttaccgctct tccagttgaa gtagattttt ttgtacgggc
     9421 atccggtgat gcacatacgc cagccacggc atttatcctg gtcgatcagg acgatgccat
     9481 cttcttcacg cttgtaaatc gcaccgctcg ggcaggtcgc aacacatgcc gggttcaggc
     9541 agtgttcgca caggcgcggc aaatacatca tgaaggtgtt ttcgaactgg ctatacatcg
     9601 ccttctggat gttgtcgaag ttcttgtctt tcgccagttt gtcaaactca ccgcccaggt
     9661 catcttccca gttcggccct ttttcgattt tcgccatccg ttccccggta atcagcgaac
     9721 gcggacgggc aatcggctgc gatttgctgc cttccggcgc ggtatgcagg ttctgatagt
     9781 caaaatcgaa cggctcgtaa taatcgtcga tccccggcag atgcgggtta gcgaagattt
     9841 tacccagcaa catggcacgg ttacccatgc gcggctgcag tttgccgttg attttacgga
     9901 tccagccgcc tttgtatttt tcctggtttt cccagtcagt cgggaagccc tgacccggct
     9961 tggtttccac gttgttgaac cacgcgtatt ccacgccttc acggctggtc cagacgtttt
    10021 tacaggtaac tgaacaggtg tggcagccga tgcacttatc gagattcagc accatgccga
    10081 cttgtgaacg aattttcatt ttacgctctc ctgtacctgg tcattgcctt cgccatctaa
    10141 ccagtcaatg ttcttcatct tacgcactac aacaaactca tcgcggttag aacctacggt
    10201 gccgtagtag ttaaagccgt atgccagatg ggcatagccg ccgatcatat gcgtcggttt
    10261 cggcgtgata cgggtgaccg agttatggat accaccacgc tgttgggtaa tttccgaacc
    10321 aggcaggtta acgatacgtt cctgcgcgtg gtacatcatg gtcatccctg ccggaacacg
    10381 ctggctgaca accgcacggg cagtcagagc accgttgctg ttgaagactt caatccagtc
    10441 gttatcggcg atacccagat ctttggcatc ggcttcactc aaccagacca ccggaccacc
    10501 gcgacctaaa gtcagcatca gcaggttgtc gctgtaggtg gagtggatac cccacttctg
    10561 gtgcggcgtc aggaagttga gcgctttttc cgggttgccg ttggatttct ggcccatcac
    10621 ttctttcacc gaacgggtgt cgatcggcgg acgataaacc agcaggcttt caccgaaatc
    10681 acgcatccat tggtgatcct gatacagctg ctgacggcca gagagcgtac gccacgggat
    10741 cagctcgtga acgttggtgt aaccggcgtt gtaagaaacg tgttcatctt caaggccaga
    10801 ccaggtcggg ctggagatga ttttgcgcgg ctgtgcctga atatcgcgga agcgaatctt
    10861 ctcgtcttct ttattcagcg ccagatgcgt atggtcacga ccggtaaact cgctcagggc
    10921 agcccaggct ttcacggcta cctgaccgtt ggtttccggt gccagcgtca ggatcatctc
    10981 tgccgcatca attgcggtgt tcagcatcgg ctggcctttc gccggacctt ccgctttggt
    11041 gtagttgagc ttacgcagca gatccatctc gctctgggtg ttccaggcaa tccctttacc
    11101 gccattaccg attttctcca tcagcgggcc gatagaggta aagcgttcgt aagtcgccgg
    11161 ataatcgcgc tctacgacca taatgtgtgg cgcggtttta ccagggatca ggtcgcactc
    11221 gccttttttc cagtctttca catccagcgg ctgcgccagt tcagcggcag agtcgtgctg
    11281 gataggcagc gtgacgatgt cggtttcttt acccagatga ccgacgcaca cttcggagaa
    11341 tttcttcgcg atggctttgt aaatttccca gtcgcttttc gcttcccagg ccggatcgac
    11401 cgccgcagac agcgggtgaa taaacggatg catatccgaa gtattcatgt cgtctttttc
    11461 gtaccaggtc gccgtcggca aaatgatgtc ggaatagaga caggtgctcg acagacggaa
    11521 gtccagcgta accaccagat ccagcttgcc ttccagacca ttgtcctgcc agtccacttc
    11581 ttccggcttc acgccgccct gttgccccag atctttcccc tggataccgt gctccgtccc
    11641 cagcaggtac ttgagcataa actcatgacc tttaccggaa gaaccgagca ggttagaacg
    11701 ccagatgaac aggttgcgcg ggtggttttt accgttttct ggttgttctg ccgcaaaacg
    11761 gatggaaccc tctttcaggg attccaccgt atagtccacc ggattcatcc cggctttttc
    11821 cgcttcgcca gcgatagtca gcgggttagt gcctaactgc ggtgcagacg gcagccagcc
    11881 catgcgttcc gcacggacgt taaagtcgat caagtgtccg gtatagcggg atttgtccgc
    11941 catcggtgac agcaactctt ccgccgtgac ggtttcataa cgccactggc tggagtggtt
    12001 atagaaataa gaagtgctgt tcatgtgacg cgccggacgc tgccagtcaa gggcaaacgc
    12061 cagcggctgc cagccggttt gcggacgcag tttttcctga cctacatagt gcgcccagcc
    12121 gcccccgctc tgaccgacac agccgcagaa aatcagcatg ttgatcagac cacgatagtt
    12181 catatcgagg tgataccagt ggttcagccc cgcaccgacg ataatcatcg aacgaccgtg
    12241 cgttttatca gcgttatcgg caaattcacg ggcgatgcga ataatctggc tgcgagaaac
    12301 gccggtaatc tgctcggccc aggccggggt ataagctttc acatcgtcat agctggttgc
    12361 acagttaacg tcgttcaggc cacgttccag accgtagttt gccagcgtca gatcataaac
    12421 ggtggtcacc agggcggtgc tgccatcagc cagttgcagg cgtttcaccg gcagtttgtg
    12481 cagcagcacg ttttccagtt ccactttgtt gaagtgttcc gtgccgtcgc caccaaagta
    12541 cgggaaacct acctcagcga tctcatcctg gctacccagc aggctcagtt gcagctcggt
    12601 ttcttcgcca gttttgccgt cgcgctgctc aagattccat ttgcccttct cgccccagcg
    12661 gaagccaata gaaccgttcg gcgcaaccat ttcgccattg gtattaaagg cgacagtttt
    12721 ccattccgga ttgttttcct ggcccagcgc atcaaccaga tcagcagcgc gcagcatacg
    12781 acctgcagcg tagtaaccgt cgcgttcttc cagcatcacc agcatcggca tgtcggtgta
    12841 gcgacgcaca tagtcggtga aatactggct tgggttgtcg aggtggaatt cacgcagcat
    12901 tacgtggccc atcgccagcg ccattgccgc atcggtgccc tgtttcggtg ccagccacag
    12961 atcgcacagt ttggcgattt cagcgtagtc tggtgtgacg gcaacagttt tggtcccttt
    13021 gtaacgcact tcagtaaaga agtgagcatc cggggtacgc gtctgcggca cgtttgaccc
    13081 ccaggcgatg atgtagctgg agttgtacca gtcagcagat tccggtacgt cagtttgctc
    13141 gccccaggtt tgcggagacg caggaggcaa gtcgcagtac cagtcgtaga agcttaagca
    13201 agtaccgcca atcagcgaga gatagcgtgc acccgatgcg taagaaacca tcgacattgc
    13261 cggaattggc gagaagccag cgacacggtc cgggccgtag tttttgatgg tgtaaacgtt
    13321 agatgcggcg atcagttcgt tcacttcctg ccaggaagaa cgaacaaaac caccgcgtcc
    13381 acgcgcctgc ttaaagcttt tcgctttatc ggcgtcttca atgatagaag cccatgcctc
    13441 aaccggatcg ctatgcagcg ccttcgcttc acgccacatt ttcatcaggc gtttgcgcat
    13501 catcgggtat ttcaggcggt tggcgctgta aagataccag gagtagctgg caccgcgcgg
    13561 gcagccgcga ggttcatggt ttggcagatc cggacgggta cgcggatagt cagtctgctg
    13621 ggtttcccag gtgaccagac cgtttttgac gtagattttc cagctgcagg agccggtgca
    13681 gtttaccccg tgggtagagc ggacgatttt gtcatgctgc caacgctggc gatatccatc
    13741 ctcccagtca cggttggtat tgagaagctg gccatgccca tcggcaaagg tttcaccctt
    13801 ctgtttgaag tagcgaaacc ggtccaggaa tttactcatc ggttttctcc tgtgggagcc
    13861 tgtcggccct ctgataaatc gacattgcta atgtttaagg gcaaggtaac gctctgaaac
    13921 agcgtgggaa ttgataacga tcaagtgtga tggggatgaa aaataaagta aattcctaag
    13981 ataccccttt aggataatta acagttaaag cataacatgc tgtaaattaa gacgttttat
    14041 acctgtagtt atttaaagca tgaagatttt tatgcagatg ggtattaagg agtattcccc
    14101 atgggtaaca tattaatggc tcttaatgaa atccggcgaa ataagccata taacattagt
    14161 atgttgtaac taaataacgc ataaaaaaag cgcgacatca tgccgcgcaa aggataatca
    14221 aatattaaga tttatttttt agaatgccga ccatataccg tccaggtaat cactacgcag
    14281 gcgatataga aaatcaaaaa taccttcatt gcgccgactg gcgaacccgt taatgccagc
    14341 gagctaccaa acgctttcgg gataaagaag ccaccaatcg cgccaatcgc agagatgaaa
    14401 cccaacgccg ccgccgtgtc ggttgccgct tcacgcatcg cacgttcgtc agaaccccct
    14461 tctgctttca cgcgatccat tgtcagttta cggaagatca ctgaaatcat ctggaaagtg
    14521 gaaccactac ccagcccagc tgtcaggaac agcgccagga agaccgcgaa gaacgccatg
    14581 aagcttccgc cctgcccgtc agtcggtaag gtcaggaaca gcaggccgct gaaaatcgcc
    14641 atcagaataa agttcaccag cgtgacacga gttccgccca gacggtcaga taatgcacca
    14701 cctgcagaac gcgccagcgc accaataaac ggcccgaaga aagcgtattg cagaatctga
    14761 acatccggga actgcgtttt tgacagcatc gcaaagcccg cggagaagcc gataaaggag
    14821 ccgaaggttg ccagatacag caggctcata atccacagat gaccccgttt gagtaccggc
    14881 aactgctcct tgatggaggc tttcgaggta gcaagatcgt tcatgccaaa ccacgccgca
    14941 atggtgaaga tggcaaggaa cggcacccat atccaggacg cattcgccag atacagctca
    15001 gtcccatccg gctgtttgac gccctggcta ccaaatactg cgaaaatcga cagtgatacc
    15061 accagcggag caaccaactg cataacgcta acgcccatgt tgcccagacc accattcagg
    15121 cccagcgcgc caccctgctt ctgtttcgga aagaagaagc tgatgtttgc catactggaa
    15181 gcgaagttcg cgccagcaaa gccgcacagc agagagatga tgatgaagac gctataaggc
    15241 gtggaggtat cctgcacggc aaaacccagc cagacacaag gaataatcag aataccggtg
    15301 ctgaacgccg tccagcgacg accaccgaag attggaacca taaaggagta tggaacacgt
    15361 aataacgcgc cagaaactga aggtagcgca gtcagcataa atagctgatc tgtcgtaaaa
    15421 ttaaagccga ctttcggtaa gttcaccgca acagcgctga acaacatcca tacgcaaaac
    15481 gccagcagca gacagggaac ggaaatccac aggttgcggc tggcaatacg ttgaccgcgt
    15541 tgttgccaga acgcaggatc ttccggtcgc caatctgtaa tgacagctcc agtagccctt
    15601 tcgggggcgg atgagtgact catagacacc tctgatactc gtttcgatgt ctgccacctt
    15661 agtgtctgta gctaaaggca atttgatgta aatcaaacga taagataact ttatctatca
    15721 ttgatattta tcattaccca tagtgagtac agtgatttca taaaaattat gagatttttc
    15781 acagtgctgt aaaatcccta cccttaccga tgtaaagcga ctaaccacac ggcaaataag
    15841 gagtaactct ttccgggtat gggtatactt cagccaatag ccgagaatac tgccattcca
    15901 gaatgtatcg tcacattcat taaggttatt gctcatttaa agcctgaagg aagaggttta
    15961 catgcttaaa cgttgtctct ctccgctcac cctggttaat caggttgcgc ttattgtgtt
    16021 gctttctact gctattggac tggcagggat ggcggtttct ggctggctgg tgcaaggcgt
    16081 tcagggcagc gcccatgcga tcaacaaagc gggatcgctg cgcatgcaaa gttaccgtct
    16141 gttggcagca gtgccattaa gcgagaaaga caagccctta attaaagaga tggaacaaac
    16201 ggcatttagc gccgagttga ctcgagcagc agaacgagac ggacaactgg cgcaattaca
    16261 gggtttacaa gattactggc gtaatgaact gatccctgcg ttgatgcgtg cacaaaaccg
    16321 agaaacggtg tcagcggatg tcagccagtt tgttgccggg cttgatcaac tggtatctgg
    16381 ttttgaccgc accacggaaa tgcgcatcga gacagtggta ctggtccatc gggtaatggc
    16441 ggtatttatg gcacttttac tggtgttcac tattatctgg ttgcgggcgc gactgctaca
    16501 accgtggcgg caactgctgg caatggcgag tgccgtcagt catcgcgatt ttacccaacg
    16561 cgcaaacatc agcgggcgca acgaaatggc gatgcttgga actgcgttga acaatatgtc
    16621 tgcagaactg gccgaaagtt atgccgtact tgagcagcgg gttcaggaga aaaccgccgg
    16681 gctggagcat aaaaatcaga tcctctcttt tttatggcag gctaaccgcc gtttgcattc
    16741 ccaagccccg ctgtgtgaac gcctgtcacc tgtactcaac ggcttacaga atttaaccct
    16801 gctacgtgat atcgaactgc gggtttatga tactgatgat gaagagaatc atcaggagtt
    16861 tacctgccaa ccagatatga cttgtgatga taaaggctgc cagctctgcc cgcgcggcgt
    16921 attacccgtt ggtgatcgcg gcacaaccct gaagtggcgg ctggctgact ctcatacgca
    16981 gtacggtatt ttgctggcga ccctgccgca ggggcgtcat cttagccatg atcaacaaca
    17041 actggtggat accctggttg aacaactcac cgccacgctg gcgctggatc gccatcagga
    17101 acgtcagcaa cagttgatcg tgatggaaga gcgtgccacc attgcgcgcg aactgcatga
    17161 ttctattgcc caatctctct cttgcatgaa gatgcaggtg agttgtttac agatgcaggg
    17221 cgatgcgctg ccagaaagca gccgcgaact gttaagtcag atccgtaacg aactgaatgc
    17281 atcctgggcg cagttgcgtg aattgctcac cacattccgc ttgcagctca ccgagcctgg
    17341 attacgtccg gcgctggagg cgagttgcga agagtacagc gccaaatttg gcttcccggt
    17401 gaagctggat tatcaattgc cgcctcgcct ggtgccttcg catcaggcaa tccacttgtt
    17461 gcaaattgcc cgtgaggcat taagtaacgc cctcaaacat tcgcaagcga gtgaagtcgt
    17521 ggtgacggtg gcgcaaaacg ataatcaggt caaactgacc gtccaggata acggctgcgg
    17581 cgtgcctgaa aatgccatcc gcagcaatca ctacggcatg ataataatgc gcgaccgtgc
    17641 gcaaagttta cgaggcgatt gccgcgtccg ccgtcgtgaa tcaggtggca ccgaagtggt
    17701 ggtcaccttt attcccgaaa aaactttcac agacgtccaa ggagataccc atgagtaatc
    17761 aggaaccggc tactatcctg ctgattgacg atcacccgat gctgcgaact ggcgtaaaac
    17821 agcttatcag tatggcacca gatatcaccg tggttggcga agcgagtaat ggcgaacagg
    17881 gtattgaact ggcggaatct cttgatcccg atctgatcct gttagatctc aatatgcccg
    17941 gcatgaacgg tctggaaacg ctggataaac tgcgcgaaaa gtccctctca gggcgcattg
    18001 tggtattcag cgtctctaac catgaagaag atgtggtcac cgcactgaaa cgcggcgcgg
    18061 atggctatct gttaaaagat atggaaccgg aagatctgct gaaagcattg catcaggcag
    18121 ctgctggcga aatggtatta agcgaagcat taacgcctgt tctggccgcc agcttgcgcg
    18181 ctaaccgtgc cactactgag cgcgatgtta accagttaac cccacgcgag cgcgatattc
    18241 tcaagctgat tgcccagggt ttgccgaaca agatgattgc ccgccgcctg gatatcaccg
    18301 aaagcacagt aaaagtgcac gtcaagcaca tgctgaagaa aatgaagctc aagtctcgcg
    18361 tggaagcagc ggtatgggtg catcaggagc gcattttctg attacggttc ccagcgcagt
    18421 tcgtcgtttg acaatgcatc gaacggttcg acaagcgtta gcgtgatctc attggaggag
    18481 acacgctgcc cctggttatc ttccaccacc accgacaatc gccagtgatt gcttgcccct
    18541 tccccgttct gccagtcagg catgatcagc gtccagccct ccgcgctgtt ggcttgtgcg
    18601 cccggcgtca aacttaatat ctgcgtatcg ccctgccaaa tcagttgccg aataccgtaa
    18661 cgactgcgga tttgtaattt cagcggcact gtttcgccag gttttagatc ccacggcggt
    18721 gtcgccagaa acaccgttaa cgtttttcgc tgtcggtact caagagtcgg tagattattt
    18781 cgctgcggat tgtcatagcg actaccacgt aacgactgac tttcggcaac ctcacccgca
    18841 gaaagttgtt ttttgagcgg tacaccaaag cggtaattaa gattcagccc gaggttattt
    18901 tgattctcgc cactttcacc ctgtttatgc tgggccgtca cagtgactaa tggcacaggg
    18961 gtgtaattta atcccagact caacgcgacg ggattgtgat aacccgtacc agagttaaac
    19021 aaatcaacac gatcaccaaa atactgttct acgctgacac tggtattgag gtgttgatag
    19081 aacggcatgc gcatccgggc tgtcaggtca tacccgcgcg ccatccgttg ttcctgcgtg
    19141 gctgtctgtt catgccatgc agcaaacggc tgataaaagt ttgccgatag tcgcaaatat
    19201 tcgccccacg cttcggcacc aaagcccgct cgctgaagat tttcgtccag caagttgtca
    19261 taaaaagtgt tataacccac cagccagttg ccgcgcgccc agcgttgccc aacgcccaca
    19321 ttgctcacca acccatcatc ctgctgagta agaccaagct ggctccaggt gagataacga
    19381 tcattatctt gtaacggcac aaaccagctt ccacgactgc cggtgaaatg tccttcgtta
    19441 tccactttga cgtcaacact ggcatttccc cacggtgata gccaggactc tacgtgctga
    19501 ttaacctgtt gactaagcgc gtcgcggact tttcccaatg cgaaagcttt tgcctgctcg
    19561 ccagtatcca gcccgttatc attcatactg gtttcgccga aatctttcac aatttcagca
    19621 aagtgttttt ccccatcatg attttcggga gccatgccta aatccggcag accatcattg
    19681 ttattatcaa agggatttgc cgctttttgc tcgaaggtag attgtgcgtt agctgtaccg
    19741 cctgctacaa gcaagagtaa ataaaacggg ataatgcgag gaacgaaacg gctcaaaacg
    19801 tcggtaactt ccggtaataa tgaaaaacca tagccttaaa gaataacgat tactggcaac
    19861 ttttgtcgta ttttctcgcg ctttcaggaa aatccttaaa aatgtgaccg attggtcaga
    19921 cgctgcagaa gcagtaagat agaagatgaa tgtctaattc cctgaataca ggagtaaata
    19981 atgcaaaaaa tcgtgatcgt tgccaatggc gcaccttacg ggagcgaatc cttgtttaac
    20041 agcttgcggc tggccattgc gttacgagag caggagagca atctggatct gcgtctgttc
    20101 ctgatgtctg atgcggtcac agccgggttg cgcgggcaaa aaccagggga aggctacaac
    20161 attcagcaaa tgctggagat ccttaccgct cagaatgtac cggtgaaatt gtgcaaaacc
    20221 tgtaccgacg ggcgcgggat tagcacactt cctcttattg atggggtgga aatcggtact
    20281 cttgtggaac tggcgcaatg gacgctgtca gccgataaag tgctcacatt ttaatgtttg
    20341 agggggcgtc tgacgcccca ctatgaggga agtttactca ggcgaatccc ggccgtaaca
    20401 caccatccgg ataattctcc gccagcagtt tttttaccga taccagcaaa tcattcagcc
    20461 catcgtcctg cattcccagt ttgataagct cctgttccag tgaaaacagg tattgtgcgt
    20521 tggttcccag cggaccgctc gccgccgcaa tcaacggcgc gatgacctga gcgcgagtgt
    20581 cagattcata ttctggatgt cgcgggtcca taataaacac aatggcgttt actgtgcatc
    20641 catcatcaag atcaagctga caccaggttg gcagataaca gccggtaatc atctcgcgct
    20701 tccacaacag ggttagttcc tgctccagcg tctcttctgg cagtcgatag gcgacgcctg
    20761 tagtgcgtcc gccctctttc agtgcaagca tccgtcccgg ctggtgcgca gtcccgcgcc
    20821 cggcggtcag gcgcaggcaa aacgcgcgat gccatccaac cagtgtaccg gtgcacgact
    20881 cggtaaactc cagtgccgga ttccacatca acgatccata accgaagatc cacaccggtc
    20941 cctcatcagg tcgacaagcc agcgtcgccg ccagcgaagc cgcccgttgc tctgctgacc
    21001 ataagagtga ttcttcaatg gcaccaaatg ccgttttaca atcggcattc atcaagaaat
    21061 cacgcgttat caccttacac ctccgccact gcatacttcc ctgcgacaac tttttggtgt
    21121 cttccggaca tttttactgc tcattttgaa accactaacg ggacaatatg caattcacgg
    21181 accacttgca aggcaacacc gcacgaatag ctttaactaa gctaccggtt ttacgatttt
    21241 ttatgccatt tatcatcatc ccctttggca tattcatgct tcacagcagc ccaggccact
    21301 ttatgcgcgg tttcttcgcg actggcgtca tcacgccgat cgtctttatc tttatattga
    21361 tcccatgcgc tgttgaacgc ttctttatag atatcctggg catgagacgg tagaacgtgc
    21421 tttacgcttt ccggcagatc gcttttcgtt ttatacggca tcgttgactc cttttacaaa
    21481 gttgaacatt aagtgtggtc aacaatatct actcttgcca aaacataaga attttcatgt
    21541 cagactgttc ttaagaaacg ctaaaacaaa cattgtctaa acgtaatact tatttttatg
    21601 tcccaatgag taaaaaatcg acagtggggt aaaaattcgt aaaatgttac cgatatccag
    21661 acactttctg ttatttttcc tacctatttt ttacattttc gataaattca tctgcaaagg
    21721 gataaccata atgtcaaatg ctcaagaggc ggtaaaaacc cgccacaagg agacttcgct
    21781 tattttcccg gttctggcgc tggtagtgct gttcctgtgg ggaagcagcc agacactacc
    21841 agtggtcatt gccatcaatc ttcttgcgct tattggtatt ttaagtagcg cctttagtgt
    21901 tgtccgtcat gcggacgtat tagcccatcg cctgggagaa ccttacggtt cgcttattct
    21961 tagcctttca gtggttattc ttgaagtcag tttgatttca gctttaatgg caaccggcga
    22021 cgccgcgcca acgctaatgc gtgatacgct ctattcaatc attatgattg ttaccggtgg
    22081 gctggttggc ttttcattat tgttgggcgg tcgtaagttt gccacccaat atatgaatct
    22141 gtttggtatc aagcagtatt taattgcgct gttccccctg gcgataatcg tactggtatt
    22201 tccaatggct ctgcctgcgg cgaatttttc aaccggtcag gcgttactgg tagcattaat
    22261 ttctgcggca atgtatggcg tatttttgct gatccagacc aaaacgcatc aaagtttgtt
    22321 tgtctacgag cacgaagatg acagtgatga cgatgacccg catcacggta aaccgtctgc
    22381 ccatagcagc gtgtggcatg ctatctggtt gattatccat ctgattgccg ttattgcagt
    22441 caccaaaatg aatgccagcc cgctggagac attgctcgac agcatgaatg cccctgtcgc
    22501 ctttactggc ttcctggtgg ctctgttgat tctgtcgccg gaaggtttag gtgcgttaaa
    22561 agcagtgttg aacaaccagg tccagcgcgc gatgaatctg ttctttggtt cagtgttagc
    22621 aaccatttcg cttaccgtac ctgtcgtcac gctaattgcc tttatgacgg gtaacgaatt
    22681 acagtttgca cttggtgcgc cagaaatggt ggtgatggtg gcctctttag tgctgtgcca
    22741 tatctccttc tccaccggac gtactaacgt gctcaatggc gcagcgcatc tggcactgtt
    22801 tgccgcctat ttgatgacga tatttgcctg acagttatat ctcctccgat gacggaaatc
    22861 gggggagata agataaattc ctgcttattg tatttcttca tactacctgg acagctcgtt
    22921 aatcagaatt gcgagcctgt accacaacct gcacgaaacc actgcaacag ctgttgaaaa
    22981 gccggaatgt actctttatt gcaaacattt cagggatgaa gtgaagatga attgagacgt
    23041 agtatccaca ccaagccgga attatccggt gaggcgcaat gttgcggggg ctttatccct
    23101 ggtggcattg gttgctggaa agagaaaacc cccgcacgtt gagaggtaaa acctgacaac
    23161 aaaacggggg ctaatcttga aaccagacac ttcaagaata gccgc
//
LOCUS       DAZFQD010000077        22603 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.077, whole genome shotgun sequence.
ACCESSION   DAZFQD010000077 DAZFQD010000000
VERSION     DAZFQD010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22603
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.077"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..108)
                     /locus_tag="ACHKTW_003000"
     CDS             complement(<1..108)
                     /locus_tag="ACHKTW_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFO6463980.1"
                     /translation="MLAKDRTNLKIEEIRMHKHHEIHRVKPLMPALCRIR"
     gene            complement(118..669)
                     /gene="ybcL"
                     /locus_tag="ACHKTW_003001"
     CDS             complement(118..669)
                     /gene="ybcL"
                     /locus_tag="ACHKTW_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Raf kinase inhibitor-like protein YbcL"
                     /protein_id="HFO6463981.1"
                     /translation="MKKLIVSSVLAFITFSAQAAAFQVTSNEIKTGEQLTTSHVFSGF
                     GCEGGNTSPSLTWSGAPEGTKSFAVTVYDPDAPTGSGWWHWTVANIPATVTYLPADAG
                     RRDGTKLPTGAVQGRNDFGYAGFGGACPPKGDKPHHYQFKIWALKTDKIPVDSNSSGA
                     LVGYMLNANKIATAEIIPVYEVK"
     gene            complement(838..1170)
                     /gene="emrE"
                     /locus_tag="ACHKTW_003002"
     CDS             complement(838..1170)
                     /gene="emrE"
                     /locus_tag="ACHKTW_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter EmrE"
                     /protein_id="HFO6463982.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGFFGQRLDLPAIIGMMLICAGVLVIN
                     LLSRSAPH"
     gene            complement(1504..1818)
                     /gene="fliE"
                     /locus_tag="ACHKTW_003003"
     CDS             complement(1504..1818)
                     /gene="fliE"
                     /locus_tag="ACHKTW_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HFO6463983.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQDSLPQPTISFAGQLHAALDR
                     ISDTQTAARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            2033..3691
                     /gene="fliF"
                     /locus_tag="ACHKTW_003004"
     CDS             2033..3691
                     /gene="fliF"
                     /locus_tag="ACHKTW_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HFO6463984.1"
                     /translation="MNATAAQTKSLEWLNRLRANPKIPLIVAGSAAVAVMVALILWAK
                     APDYRTLFSNLSDQDGGAIVSQLTQMNIPYRFSEASGAIEVPADKVHELRLRLAQQGL
                     PKGGAVGFELLDQEKFGISQFSEQVNYQRALEGELSRTIATIGPVKGARVHLAMPKPS
                     LFVREQKSPSASVTVNLLPGRALDEGQISAIVHLVSSAVAGLPPGNVTLVDQGGHLLT
                     QSNTSGRDLNDAQLKYASDVEGRIQRRIEAILSPIVGNGNIHAQVTAQLDFASKEQTE
                     EQYRPNGDESHAALRSRQLNESEQSGSGYPGGVPGALSNQPAPANNAPISTPPANQNN
                     RQQQASTTSNSGPRSTQRNETSNYEVDRTIRHTKMNVGDVQRLSVAVVVNYKTLPDGK
                     PLPLSNEQMKQIEDLTREAMGFSEKRGDSLNVVNSPFNSSDESGGELPFWQQQAFIDQ
                     LLAAGRWLLVLLVAWLLWRKAVRPQLTRRAEAMKAVQQQAQAREEVEDAVEVRLSKDE
                     QLQQRRANQRLGAEVMSQRIREMSDNDPRVVALVIRQWINNDHE"
     gene            3684..4679
                     /gene="fliG"
                     /locus_tag="ACHKTW_003005"
     CDS             3684..4679
                     /gene="fliG"
                     /locus_tag="ACHKTW_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HFO6463985.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLKRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLANRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            4672..5358
                     /gene="fliH"
                     /locus_tag="ACHKTW_003006"
     CDS             4672..5358
                     /gene="fliH"
                     /locus_tag="ACHKTW_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HFO6463986.1"
                     /translation="MSDNLPWKTWTPDDLAPPQAEFVPMVEPEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHEQGYQEGLARGLEQGLAEAKSQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            5358..6731
                     /gene="fliI"
                     /locus_tag="ACHKTW_003007"
     CDS             5358..6731
                     /gene="fliI"
                     /locus_tag="ACHKTW_003007"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HFO6463987.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSDKPLDGLPSPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            6750..7193
                     /gene="fliJ"
                     /locus_tag="ACHKTW_003008"
     CDS             6750..7193
                     /gene="fliJ"
                     /locus_tag="ACHKTW_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HFO6463988.1"
                     /translation="MAEHGALATLKDLAEKEVEDAARLLGEMRRGCQQAEEQLKMLID
                     YQNEYRNNLNSDMSAGMTSNRWINYQQFIQTLDKAITQHRQQLNQWTQKVDIALNSWR
                     EKKQRLQAWQTLQERQSTAALLAENRLDQKKMDEFAQRAAMRKPE"
     gene            7190..8317
                     /gene="fliK"
                     /locus_tag="ACHKTW_003009"
     CDS             7190..8317
                     /gene="fliK"
                     /locus_tag="ACHKTW_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HFO6463989.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLALLSEALAGETTTD
                     KAAPQLLVATDKPTTKGELLISDIVSDAQQADLLIPVDETLPVINVEQSTSTPLTTAQ
                     TMTLAAVADKNTTKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDVPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTAAASPLITPH
                     QTQPLPTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMVSPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTVNHEPLAGEDDDTLPVPVSIQGRVTGNSGVDIFA"
     gene            8422..8886
                     /gene="fliL"
                     /locus_tag="ACHKTW_003010"
     CDS             8422..8886
                     /gene="fliL"
                     /locus_tag="ACHKTW_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HFO6463990.1"
                     /translation="MTDYAISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAADD
                     KAQQRVVPSPVFYALDTFTVNLGDADRVLYIGVTLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            8891..9895
                     /gene="fliM"
                     /locus_tag="ACHKTW_003011"
     CDS             8891..9895
                     /gene="fliM"
                     /locus_tag="ACHKTW_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HFO6463991.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTER
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVKFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            9892..10305
                     /gene="fliN"
                     /locus_tag="ACHKTW_003012"
     CDS             9892..10305
                     /gene="fliN"
                     /locus_tag="ACHKTW_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HFO6463992.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSSKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            10308..10673
                     /gene="fliO"
                     /locus_tag="ACHKTW_003013"
     CDS             10308..10673
                     /gene="fliO"
                     /locus_tag="ACHKTW_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HFO6463993.1"
                     /translation="MNNHATVQSSAPVSAAPLLQVSGALIAIIALILAAAWLVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRSGRS"
     gene            10673..11410
                     /gene="fliP"
                     /locus_tag="ACHKTW_003014"
     CDS             10673..11410
                     /gene="fliP"
                     /locus_tag="ACHKTW_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HFO6463994.1"
                     /translation="MRRLLSVAPVLLWLVTPLAFAQLPGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     PVIDKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            11420..11689
                     /gene="fliQ"
                     /locus_tag="ACHKTW_003015"
     CDS             11420..11689
                     /gene="fliQ"
                     /locus_tag="ACHKTW_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HFO6463995.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            11698..12483
                     /gene="fliR"
                     /locus_tag="ACHKTW_003016"
     CDS             11698..12483
                     /gene="fliR"
                     /locus_tag="ACHKTW_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HFO6463996.1"
                     /translation="MMQVTSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFAIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPASHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     GEPLNSNAFLALTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEMFNLLADIISELPLI"
     gene            12773..13396
                     /gene="rcsA"
                     /locus_tag="ACHKTW_003017"
     CDS             12773..13396
                     /gene="rcsA"
                     /locus_tag="ACHKTW_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HFO6463997.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQHIKHIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(13440..13628)
                     /gene="dsrB"
                     /locus_tag="ACHKTW_003018"
     CDS             complement(13440..13628)
                     /gene="dsrB"
                     /locus_tag="ACHKTW_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HFO6463998.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVSLEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            13791..14018
                     /gene="yodD"
                     /locus_tag="ACHKTW_003019"
     CDS             13791..14018
                     /gene="yodD"
                     /locus_tag="ACHKTW_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HFO6463999.1"
                     /translation="MKTAKEYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            14316..15131
                     /gene="yedP"
                     /locus_tag="ACHKTW_003020"
     CDS             14316..15131
                     /gene="yedP"
                     /locus_tag="ACHKTW_003020"
                     /EC_number="3.1.3.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050531 - mannosyl-3-phosphoglycerate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0051479 - mannosylglycerate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
                     /protein_id="HFO6464000.1"
                     /translation="MFSIQQPLLVFSDLDGTLLDSHSYDWQPAAPWLSRLREANVPVI
                     LCSSKTSAEMLYLQKTLGLQGLPLIAENGAVIQLAEQWQDIDGFPRIISGISHGEISQ
                     VLNTLREKEHFKFTTFDDVDDATIAEWTGLSRSQAALTQLHEASVTLIWRDSDERMAQ
                     FTARLNELGLQFMQGARFWHVLDASAGKDQAANWIIATYQQLSGKRPTTLGLGDGPND
                     APLLEVMDYAVIVKGLNREGVHLHDEDPARVWRTQREGPEGWGEGLDHFFSAR"
     gene            complement(15128..16822)
                     /gene="dgcQ"
                     /locus_tag="ACHKTW_003021"
     CDS             complement(15128..16822)
                     /gene="dgcQ"
                     /locus_tag="ACHKTW_003021"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator diguanylate
                     cyclase DgcQ"
                     /protein_id="HFO6464001.1"
                     /translation="MQHETKMENQSWLKKLARRLGPGHVVNLCFIVVLLFSTLLTWRE
                     VVVLEDAYISSQRNHLENVANALDKHLQYNVDKLIFLRNGMREALVAPLDFTSLRDAV
                     TEFEQHRDEHAWKIELNRRRTLSVNGVSDALVSEGNLLSRENESLDNEITAALEVGYL
                     LRLAHNTSSMVEQAMYVSRAGFYVSTQPTLFTRNVPTRYYGYVTQPWFIGHSQRENRH
                     RAVRWFTSQPEHASNTEPQVTVSVPVDSNNYWYGVLGMSIPVRTMQQFLRNAIDKNLD
                     GEYQLYDSKLRFLTSSNPDHPTGNIFDPRELALLAQAMEHDTRGGIRMDSRYVSWERL
                     DHFDGVLVRVHTLSEGVRGDFGSISIALTLLWALFTTMLLISWYVIRRMVSNMYVLQS
                     SLQWQAWHDTLTRLYNRGALFEKARPLAKLYQTHQHPFSVIQVDLDHFKAINDRFGHQ
                     AGDRVLSHAAGLISSSLRAQDVAGRVGGEEFCVILPGASLTEAAEVAERIRLKLNEKE
                     MLIAKSTTIRISASLGVSSSEETGDYDFEQLQSLADRRLYLAKQAGRNRVCASDNA"
     gene            complement(17060..17242)
                     /gene="yodC"
                     /locus_tag="ACHKTW_003022"
     CDS             complement(17060..17242)
                     /gene="yodC"
                     /locus_tag="ACHKTW_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HFO6464002.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETELVPGEGSRPAEEV"
     gene            complement(17321..18238)
                     /gene="yedI"
                     /locus_tag="ACHKTW_003023"
     CDS             complement(17321..18238)
                     /gene="yedI"
                     /locus_tag="ACHKTW_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HFO6464003.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKVKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHFAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKIRGQAH"
     gene            18411..19331
                     /gene="yedA"
                     /locus_tag="ACHKTW_003024"
     CDS             18411..19331
                     /gene="yedA"
                     /locus_tag="ACHKTW_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HFO6464004.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVESWPPLMMAGVRF
                     LAAGILLMAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     VATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIQDASSE"
     gene            complement(19320..19790)
                     /gene="vsr"
                     /locus_tag="ACHKTW_003025"
     CDS             complement(19320..19790)
                     /gene="vsr"
                     /locus_tag="ACHKTW_003025"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HFO6464005.1"
                     /translation="MADVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGRPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRGREKLTDEALSERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(19771..21189)
                     /gene="dcm"
                     /locus_tag="ACHKTW_003026"
     CDS             complement(19771..21189)
                     /gene="dcm"
                     /locus_tag="ACHKTW_003026"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HFO6464006.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKTLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPAHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHKEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDQGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMAKGEKDFDDP
                     QNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            complement(21256..21951)
                     /gene="yedJ"
                     /locus_tag="ACHKTW_003027"
     CDS             complement(21256..21951)
                     /gene="yedJ"
                     /locus_tag="ACHKTW_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HFO6464007.1"
                     /translation="MDLQHWQAQFENWLKNHHQHQDAAHDVCHFRRVWATAQKLAADD
                     DVDMLVILTACYFHDIVSLAKNHPQRQRSSILAAEETRRLLREEFEQFPAEKIEAVCH
                     AIAAHSFSAQIAPLTTEAKIVQDADRLEALGAIGLARVFAVSGALGVALFDGEDPFAQ
                     HRPLDDKRYALDHFQTKLLKLPQTMQTARGKQLAQHNAHFLVEFMAKLSAELAGENEG
                     VDHKVIDAFSSAG"
     gene            complement(21991..22272)
                     /gene="drpB"
                     /locus_tag="ACHKTW_003028"
     CDS             complement(21991..22272)
                     /gene="drpB"
                     /locus_tag="ACHKTW_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DrpB"
                     /protein_id="HFO6464008.1"
                     /translation="MEERLSRSPGGKLALWAFYTWCGYFVWAMARYIWVMSRIPDAPV
                     SGFESDLGSTAGKWLGALVGFLFMALVGALLGSIAWYTRPRPARSRRYE"
BASE COUNT         5520 a         5939 c         5747 g         5397 t
ORIGIN      
        1 acggatacga cataaagctg gcataagagg cttaaccctg tgaatctcat gatgtttatg
       61 catccgtatt tcttcaatct ttaggttagt cctatctttt gccagcatac tcttatccta
      121 ctttacctca taaacgggta ttatctcagc ggttgcaatt ttattggcat taagcatata
      181 accaactaac gctccgctgg aattagaatc cacaggaatc ttatcagttt ttagagccca
      241 tattttaaac tggtaatgat ggggtttatc tcctttggga ggacatgctc caccaaatcc
      301 cgcatagcca aaatcatttc ggccttggac agcaccagtc ggaagttttg taccatcacg
      361 tcttcctgca tcagcgggca aatatgttac cgttgctgga atattagcaa cagtccagtg
      421 ccaccatccg ctgcctgtag gcgcatctgg atcatacaca gttacggcaa agcttttggt
      481 gccttctgga gcaccagacc aggttaatga gggcgatgta ttacccccct cacacccaaa
      541 tccagaaaat acatgagacg ttgtaagttg ctctcctgtt tttatttcat tactagtgac
      601 ctgaaatgct gcggcctgcg cagaaaatgt tatgaatgcc aatacacttg aaacgataag
      661 ttttttcata aaaacctctt tgttgtgacc tatcgttatt ttatttgatg tgcattgatc
      721 tcattatgca aaaaggcgca atgatcgcgc aaatgcaatc ataattataa agttgccccg
      781 aacattagcc ataatatgca caaatcagat tcctgttatc tcaaggatga ttatttttta
      841 atgcggtgcg cttcgtgaca acaagttaat gactaacaca ccggcacaaa tcaacatcat
      901 gcctatgatg gctggcaagt ccagtcgttg gccaaaaaat ccccaggaaa gcaaactaat
      961 cagaacgata ccgactcctg accagatagc ataagcaatc cctgtaggta tataagccag
     1021 tgtctgagct aataaccaaa atgatgcaca atagcaaata attgtgccaa cagagggcca
     1081 taaccgtgta aacccctctg aaaacttcat taaggttgta ccaatgacct ctgcaagtat
     1141 tgcgccacca agataaatat aagggttcat agcatattct ttcctgttca tactggagag
     1201 aattgtacta cagtttgaac acaactcacc gttttcatcc tggtgtatgt gtatccaggg
     1261 ggctctggcg tggcaagaga taaaggcagg aacaataatg atcagaagga accccaggct
     1321 aacaaaagag ttcactcttc ctatgagcaa agcgaagaca aaacatgtta tcgcgtatac
     1381 ggatcgtgga tggcagaaaa ctttcaggat caggtatcca ttttaaacca gaaatagggt
     1441 gagtttgccc catccatgcc ccacgcgggc agatgggatg taattaacaa caggttacaa
     1501 aacctacact tgcatggaca tcacttcctg atacgccgcc accagcttat tacgcacctg
     1561 aatccccatt tgcatagaaa ctgaggcttt ttgcatatcg gtcatcacat cgtttaacgc
     1621 cacgccgggt tcaccgaggg tgaatttttc tgcctgcgtg cgggcagctg tttgtgtatc
     1681 gctaatgcga tcgagcgcgg cgtgcagctg cccggcaaaa ctaatggtcg gttgcggcag
     1741 tgaatcctgc gcacgcgcac tcatcgccgt agcctgtaac tggcttataa ccccttcaat
     1801 cccctgtatc gctgacattt tcgtctcctg gataatttct ggtagcaaag cctaccagta
     1861 agtcaataag acaaaggcgc taaatagcaa caaaaaaacg ggtttattgg cggatagaaa
     1921 aaaacgaaag cacaaataat ggcagcgtca atttttcgag tttgctgacc cgggagtgag
     1981 tcttgttcca ctttgccaat aacgccgtcc ataatcagcc acgaggtgcg cgatgaatgc
     2041 gactgcagcc cagacaaaat ctcttgagtg gcttaatcgc ctgcgtgcga atccgaaaat
     2101 tccattgatt gttgccggtt ccgcggcagt ggcggtcatg gtcgcactga tcctgtgggc
     2161 gaaagccccc gactaccgca cattattcag caatctttcc gatcaggatg gtggcgcaat
     2221 tgtcagccaa ctgacgcaaa tgaatattcc ttaccgcttc agcgaagcca gcggcgctat
     2281 tgaagttccg gcagataaag ttcacgaact gcgtctgcgc ctggcacaac aaggtttgcc
     2341 caaaggcggc gcggtcggtt tcgaactact cgatcaggaa aagtttggta tcagccagtt
     2401 cagcgaacag gtgaattatc agcgggcgct ggaaggcgag ctttctcgta ccatcgcaac
     2461 tatcggcccg gtaaaagggg cgcgcgtaca tctggcaatg ccgaaaccgt ctttattcgt
     2521 ccgtgaacaa aaatcccctt ctgcatcggt gacggtaaat ctgttacccg gccgcgcact
     2581 cgatgaaggg caaattagcg ccattgtgca tctggtttcc agcgccgttg ctggtctgcc
     2641 gccgggaaac gtcacgctgg tggatcaggg cggacatctg ttaacccagt ccaataccag
     2701 cgggcgcgat cttaatgatg ctcagttgaa atatgccagc gatgtcgaag gccgtattca
     2761 gcggcgtatt gaagcaatcc tgtcgcctat tgttggtaac ggtaatattc acgcccaggt
     2821 cacggcgcag ctggacttcg ccagtaaaga acaaacggaa gaacagtatc gccctaacgg
     2881 tgatgaatct catgcggcgc ttcgttcacg ccagcttaat gagagcgagc aaagcggttc
     2941 cggttatccg ggcggcgtac cgggggcgtt gtcgaatcaa ccggcacctg cgaataacgc
     3001 gccaatcagc acgcctccgg caaatcaaaa taaccgccag cagcaggcga gcaccaccag
     3061 caatagcggg ccgcgtagca cacagcggaa tgaaaccagt aactacgaag tcgatcgcac
     3121 cattcgtcat accaaaatga acgtgggcga tgtgcaacgt ctgtcagtcg cggtagtggt
     3181 gaattacaaa accttgccag acggtaaacc attgcctctc agcaacgaac agatgaagca
     3241 aattgaagat ctgacccgcg aggcgatggg cttttctgaa aaacgcggtg actcgctcaa
     3301 tgtcgttaac tcgccgttca atagcagtga cgaaagcggc ggagaactgc cgttctggca
     3361 acagcaagcg tttatcgatc agttgctggc tgccggtcgc tggttgctgg tactgctggt
     3421 ggcgtggctg ctgtggcgga aagcggtacg cccgcagtta acacgtcgcg ccgaggcgat
     3481 gaaagctgta cagcaacagg cgcaggcccg tgaggaagtg gaagatgcgg tggaagtccg
     3541 cctgagcaaa gacgaacaac tccaacaacg acgcgctaac caacgtctgg gggcagaagt
     3601 catgagccag cgtatccgtg aaatgtctga taacgatccg cgcgtggtgg cgctggtcat
     3661 tcgccagtgg ataaataacg atcatgagta acctgacagg caccgataaa agcgtcatcc
     3721 tgctgatgac cattggcgaa gaccgggcgg cagaggtgtt caagcacctc tcccagcgcg
     3781 aagtgcaaac cctgagcgct gcaatggcga acgtcacgca gatctccaac aagcagctaa
     3841 ccgatgtgct ggcggagttt gagcaagaag ctgaacagtt tgccgcactg aatatcaacg
     3901 ccaacgatta tctgcgttcg gtattggtca aagctctggg tgaggaacgt gccgccagcc
     3961 tgctggaaga tattctcgaa actcgcgata ccgccagcgg tattgaaacg ctcaacttta
     4021 tggagccgca gagtgccgcc gatctgattc gcgatgagca tccgcaaatt atcgccacca
     4081 ttctggtgca tctgaagcgc gcccaggccg ccgatattct ggcgttgttc gatgaacgtc
     4141 tgcgccacga cgtgatgttg cgtatcgcca cctttggcgg cgtgcagcca gccgcgctgg
     4201 cggaactgac cgaagtactg aatggcttgc tcgacggtca gaatctcaag cgcagcaaaa
     4261 tgggcggcgt gagaacggca gccgaaatta tcaacctgat gaaaactcag caggaagaag
     4321 ccgttattac cgccgtgcgc gaattcgacg gcgagctggc gcaaaaaatt atcgacgaga
     4381 tgttcctgtt cgaaaatctg gtggatgtcg acgatcgcag cattcagcgt ctgttgcagg
     4441 aagtggattc cgaatcgctg ttgatcgcgc tgaaaggggc cgagcagcca ctgcgcgaga
     4501 aattcctgcg caatatgtcg cagcgtgccg ccgatattct gcgcgacgat ctcgccaacc
     4561 gtggtccggt gcgtctgtcg caggtggaaa acgaacagaa agcgattctg ctgattgtgc
     4621 gccgccttgc cgaaactggc gagatggtaa ttggcagcgg cgaggatacc tatgtctgat
     4681 aatctgccgt ggaaaacctg gacgccggac gatctcgcgc caccacaggc agagtttgtg
     4741 cccatggtcg agccggaaga aaccatcatt gaagaggccg aacccagcct tgagcagcaa
     4801 ctggcgcaac tgcaaatgca ggcccatgag caaggttatc aggcggggat tgccgaaggt
     4861 cgccagcaag gtcatgagca gggctatcag gaaggactgg cccgggggct ggagcaaggt
     4921 ctggcagagg cgaagtctca acaagcgcca attcatgccc ggatgcagca actggtcagc
     4981 gaatttcaaa ctacccttga tgcgcttgat agtgtgattg cttcgcgcct gatgcagatg
     5041 gcgctggagg cggcacgtca ggtcatcggt cagacgccaa cagtggataa ctcggcactg
     5101 atcaaacaga tccaacagtt gttgcagcaa gaaccgttat tcagcggtaa accacagctg
     5161 cgcgtgcacc cggatgatct gcaacgtgtg gatgatatgc tcggcgcaac cttaagtttg
     5221 catggctggc gcttgcgggg cgatcccaca ctccatcctg gcggctgtaa agtctccgcc
     5281 gatgaaggcg atctcgacgc cagtgtcgcc actcgctggc aagaactctg ccgtctggca
     5341 gcaccaggag tggtgtaatg accacgcgcc tgactcgctg gctaaccacg ctggataact
     5401 ttgaagccaa aatggcgcag ttgcctgcgg tacgtcgcta tgggcgatta acccgcgcta
     5461 ccgggctggt gctggaagcc accggattac aattgccgct cggcgcaacc tgtgtcattg
     5521 aacgccagaa cggcagcgaa acgcacgaag tagaaagcga agtcgttggc tttaacggtc
     5581 aacggctgtt tttaatgccg ctggaggaag tcgaaggtgt cctgcccggc gcgcgggttt
     5641 atgccaaaaa catttcggct gaaggactgc aaagcggcaa gcagttgccg ctcggtccgg
     5701 cgttattagg tcgcgttctg gacggcagcg ataagccgct cgatggcctg ccctcccccg
     5761 atacgacgga aaccggtgcg ctgattaccc cgccatttaa cccgttgcaa cgtacaccga
     5821 ttgaacatgt gctggacacc ggcgtgcgcc caatcaatgc cctgcttacc gttgggcgtg
     5881 ggcagcgtat gggactgttt gccgggtccg gcgttggtaa aagtgtgctg ctggggatga
     5941 tggcacgtta cacccgcgcc gatgtcattg tcgtgggttt gattggtgaa cgcggacgcg
     6001 aagtaaaaga ttttattgag aatatcctcg gtgccgaagg gcgtgcacgc tccgtggtga
     6061 ttgccgctcc ggcggatgtt tctcctctcc tgcgaatgca gggtgccgcc tatgccacgc
     6121 gcattgccga agattttcgc gatcgtggtc agcatgtgtt gctgattatg gactccctca
     6181 cccgctacgc gatggcccag cgtgaaattg cgctggcgat tggcgaacca cccgccacta
     6241 aaggttatcc cccgtcggtg tttgccaaat taccggcact ggtcgagcgt gccggaaatg
     6301 gcattagcgg cggcggatcg attactgcat tttataccgt gctcactgaa ggcgatgacc
     6361 agcaggaccc cattgccgac tctgcgcggg caatcctcga cggtcacatt gtgctgtctc
     6421 gccgactggc ggaagccggg cactatccgg ctatcgatat tgaagcgtcg atcagccgcg
     6481 caatgacggc gttgatcagt gagcaacatt acgcgcgagt gcgcaccttc aaacagctgt
     6541 tgtcgagttt tcagcgtaac cgcgatctgg ttagcgtcgg cgcgtatgcc aaaggcagcg
     6601 atccgatgct cgacaaagcc atcgccctgt ggccgcagct ggagggctat ttgcaacaag
     6661 gcatttttga acgcgcggac tgggaagcgt ctctccaggg gctggagcgt attttcccga
     6721 cagtgtcata acccaggaga taacggcaga tggcagaaca tggcgcgctg gcgaccctga
     6781 aagatctggc agaaaaagag gtagaggatg ccgcgcgcct gctgggtgaa atgcgtcgcg
     6841 ggtgtcagca ggcggaagaa cagctcaaaa tgctgatcga ttatcagaat gaatatcgca
     6901 ataacctcaa cagcgatatg agtgccggga tgaccagcaa ccgctggatc aactatcagc
     6961 agtttatcca gacgctggat aaagccatta ctcagcatcg ccagcaactt aatcagtgga
     7021 cgcagaaagt tgacattgcc ctgaacagct ggcgagaaaa gaaacaacgt ttgcaggcct
     7081 ggcagacact gcaggaacgg caatccacgg cggcactgct tgcagaaaac cgcctcgatc
     7141 agaaaaagat ggatgagttc gcccagcgcg ccgccatgag gaaacctgaa tgattcgctt
     7201 agcgccctta attaccgccg acgttgacac caccacattg cctggcggca aagccagcga
     7261 tgctgcacaa gattttctcg cgttgttgag cgaagcatta gcaggcgaga caactaccga
     7321 caaagcggcc ccccagttgc tggtggcaac agataagccc acgacaaaag gcgagctgct
     7381 gatcagcgat attgtttccg acgcgcaaca agcggattta ctgatccctg tggatgaaac
     7441 actgcctgtc atcaacgtcg aacaatccac atcaacaccg ttaaccaccg ctcagacgat
     7501 gacgttggct gcggtggctg acaaaaatac gacaaaagac gaaaaagcgg atgatctgaa
     7561 tgaagacgtc accgcaagcc tgagcgccct ttttgcgatg ttgccgggtt ttgacaatac
     7621 gcccaaagtg actgatgtgc cgtcaaccgt gttaccggca gagaaaccaa cgctcttcac
     7681 aaaactgact tctgcgcaac tcacaacagc acagcctgat gatgcccccg gcacgccagc
     7741 tcagccatta acaccgctgg tagcagaagc ccagagtaaa gcggaagtca tcagcacacc
     7801 gtcaccggtg accgctgccg ccagcccgct aattactcca caccagacac agccactgcc
     7861 caccgtcgcc gcgcctgttt tgagtgcacc gctgggttct cacgaatggc aacaatcatt
     7921 aagccagcat atttcgctat tcacccgcca ggggcaacaa agtgcagagt tgcgtctgca
     7981 cccgcaggat ttaggtgaag tgcaaatctc cctcaaagtg gatgataacc aggcacaaat
     8041 ccagatggtt tcaccgcatc agcatgtacg cgccgcgctg gaagcagcgc ttccggtact
     8101 gcgtacgcag ctggccgaaa gtggcattca gttagggcaa agcaacatca gtggcgaaag
     8161 ctttagtggt cagcagcagg ccgcttccca acaacagcaa agccaacgca cagtaaacca
     8221 tgaacctctg gcgggggaag acgacgatac gcttccggtt cccgtctcta tacaagggcg
     8281 cgtaacaggc aacagcggcg ttgatatttt cgcctaacgt cagaggtagc accgtaatcc
     8341 gcgtcttttc cccgctttgt tgcgctcaag acgcaggata attagccgat aagcagtagc
     8401 gacacaggaa gaccgcaaca catgactgat tacgcgataa gcaagaaaag caagcgatcg
     8461 ctttggatcc cgattctggt attcattacc ctcgcggcct gtgccagcgc aggttacagc
     8521 tactggcatt cgcatcaggt tgccgctgac gacaaagcgc agcaacgcgt cgtaccctcc
     8581 ccggtcttct acgcgctgga taccttcacg gtcaatttgg gcgatgcgga tcgcgtgctt
     8641 tatatcggcg taaccctgcg cctgaaagat gaagctaccc gctcgcggct gagtgagtat
     8701 ttgccggaag tccgtagccg tttgctgtta ctgttttcgc gtcaggatgc tgccgtactg
     8761 gcgacagaag aaggcaagaa aaacctgatt gccgagatta aaaccacgct ttccaccccg
     8821 cttgttgccg ggcaaccgaa acaggatgtc accgacgtgc tgtataccgc ttttattctg
     8881 cgataacgac atgggcgata gtattctttc tcaagctgaa attgatgcgc tgttgaatgg
     8941 tgacagcgaa gtcaaagacg aaccgacagc cagtgttagc ggcgaaagtg acattcgtcc
     9001 gtacgatccg aataccgaac gacgggttgt gcgcgaacgt ttgcaggcgc tggaaatcat
     9061 taatgagcgc tttgcccgcc attttcgtat ggggctgttc aacctgctgc gtcgtagccc
     9121 ggatataacc gtcggggcca tccgcattca gccgtaccat gaatttgccc gcaacctgcc
     9181 ggtgccgacc aacctgaacc ttatccatct gaaaccgctg cgcggcactg ggctggtggt
     9241 gttttcaccg agtctggtgt ttatcgccgt ggataacctg tttggcggcg atggacgctt
     9301 cccgaccaaa gtggaaggtc gcgagtttac ccataccgaa cagcgcgtca tcaaccgcat
     9361 gttaaagctg gcgcttgaag gctatagcga cgcctggaag gcgattaatc cgctggaagt
     9421 tgagtacgtg cgttcggaaa tgcaggtgaa atttaccaat atcaccacct cgccgaacga
     9481 cattgtggtt aacacgccgt tccatgtgga gattggcaac ctgaccggcg aatttaatat
     9541 ctgtctgcca ttcagcatga ttgagccgct acgggaattg ttggttaacc cgccgctgga
     9601 aaactcgcgt aatgaagatc agaactggcg cgataacctg gtgcgccagg tgcagcattc
     9661 acagctggag ttggtcgcca actttgccga tatctcgcta cgcctgtcgc agattttaaa
     9721 actgaaacca ggcgacgtcc tgccgataga aaaacccgat cgcatcatcg ctcatgttga
     9781 cggcgttccg gtactgacca gtcagtacgg caccctcaac ggtcagtatg cgttacggat
     9841 agaacatttg attaacccga ttttaaattc tctgaacgag gaacagccca aatgagtgac
     9901 atgaataatc cggccgatga caacaacggc gcaatggacg atctgtgggc tgaagcgttg
     9961 agcgaacaaa aatcaaccag cagcaaaagc gctgccgacg cggtgttcca gcaatttggc
    10021 ggtggtgatg tcagcggaac gttgcaggat atcgacctga taatggatat tccggtcaag
    10081 ctgaccgtcg agctgggccg tacgcggatg accatcaaag agctgttgcg tctgacgcaa
    10141 gggtccgtcg tggcgctgga cggtctggcg ggcgaaccac tggatattct gatcaacggt
    10201 tatttaatcg cccagggcga agtggtggtc gttgccgata aatatggcgt gcggatcacc
    10261 gatatcatta ctccgtctga gcgaatgcgt cgcctgagcc gttagtgatg aataaccacg
    10321 ctactgtgca atcttccgcg ccggtttctg ctgcgccact gctgcaggtc agcggcgcac
    10381 tcatcgccat tattgccctg atcctcgctg ctgcctggct ggtaaaacgg ttgggatttg
    10441 ccccgaaacg cactggcgtt aacggtctga aaattagcgc cagtgcttcg ctgggcgcgc
    10501 gtgaacgggt tgtggtggtc gatgtggaag atgcacggct ggtgctcggc gttaccgcag
    10561 ggcaaatcaa tctgctgcat aaacttcccc cttcagcacc aacagaagag ataccgcaga
    10621 ccgattttca gtcggtcatg aaaaatttgc ttaagcgtag cgggagatcc tgatgcgtcg
    10681 tttgttgtct gtcgcacctg tccttctctg gctggttacg cccctcgcct tcgcgcaact
    10741 gccgggtatc accagccagc cgctgcctgg cggtggacaa agctggtcgc tcccggtgca
    10801 gacgctggtg ttcatcacct cgttgacgtt tattccggca attttactga tgatgaccag
    10861 tttcacccgc atcatcattg tttttggttt attgcgtaac gcgctgggaa cgccctccgc
    10921 gccacctaac caggtattgc tggggctggc actatttttg acctttttta ttatgtcacc
    10981 ggtgatcgac aaaatttatg tcgatgcgta ccagccattc agcgaagaga aaatatcaat
    11041 gcaggaggcg ctggaaaaag gggcgcagcc gctacgtgag tttatgctgc gtcagacccg
    11101 tgaggcagat ttagggttgt ttgccagact agcgaatacc ggcccgttgc agggacctga
    11161 agccgtgccg atgcgcattt tgctcccggc ctacgtgacc agcgagttga aaaccgcatt
    11221 tcagataggc ttcacgattt tcatcccttt tttgattatc gacctggtga ttgccagcgt
    11281 gttgatggca ttggggatga tgatggtccc ccccgccacc attgctctgc cctttaaact
    11341 gatgctgttt gtactggtgg atggctggca attgctggtc ggttcgctgg cgcagagctt
    11401 ttacagctag agaggcaaaa tgacacctga atcggtcatg atgatgggga ctgaagcgat
    11461 gaaagtcgcg ctggcactgg ctgccccgct gttgctggtg gcgctggtca cgggccttat
    11521 catcagtatt ttgcaggccg ccacccagat caacgaaatg acgctgtcgt ttattccaaa
    11581 aatcatcgcc gtatttattg ccattattat tgccggaccg tggatgctca atctgttgct
    11641 ggattacgtc cgcaccttgt tcactaacct gccgtatatc atcgggtagc ccgtacaatg
    11701 atgcaggtga caagcgatca atggctttcc tggctaagtc tgtacttctg gccgttactg
    11761 cgcgtgctgg cgctcatttc taccgcgcca attctgagcg aacgcagcgt gccgaaacgg
    11821 gtaaaactgg gtctggcaat gatgatcacg ttcgctattg ccccatcatt acctgccaac
    11881 gatgttcctg ttttttcgtt ctttgctctg tggctggccg tgcagcagat cctgatcggc
    11941 attgcgcttg gttttaccat gcaatttgcc tttgccgctg tgcgaaccgc tggcgaaatt
    12001 atcggtctgc aaatggggct gtcatttgcg acgtttgtcg atccggccag ccatctcaat
    12061 atgcctgttt tagcgcgtat catggatatg ctggcgttac tactgttcct gacatttaac
    12121 ggtcatttat ggttgatttc actgctggtc gatacctttc acaccctgcc aattggtggc
    12181 gaaccgttga acagcaatgc gtttctggca ctcaccaaag cagggagttt gattttcctt
    12241 aacgggctga tgctggcgtt accgctcatt actctgctgc tgacactgaa tctggcatta
    12301 ggtttactta atcgtatggc cccgcaatta tccatttttg ttattggatt tccattaact
    12361 ctgactgtcg gtatctcttt aatggcggca ttaatgccgt taattgcacc tttttgcgaa
    12421 catttattca gtgagatgtt taatttgctg gctgatatta ttagtgaatt gccattaata
    12481 taattccgta acgtttatca tgttatccta aggattatcc gaaaaataat acctgcgaac
    12541 atcttccagg atactcctgt ggcgaaatat ttgttttaag ctcactcaca tatcgcaaca
    12601 tttactttac tttaagacaa ttccaggcaa attatacaac actttacggg atagtaagtc
    12661 cgcctgaaaa atcgcgagag tggcgcatta ggtgacccat gttgttccgt ttagtcatga
    12721 tgaaatattc aggtaagggg aattatcgtt acgcattgag tgagggtatg ccatgtcaac
    12781 gattattatg gatttatgta gttacacccg actaggttta accgggtatc tgttgagtag
    12841 aggggttaaa aaaagagaaa tcaacgacat tgaaaccgtt gatgaccttg ccatagcttg
    12901 tgattcacag cgcccttcag tggtgtttat taatgaggac tgtttcatcc atgatgcgtc
    12961 taacagtcag cacatcaagc acatcattaa tcaacatccc aatacgttat ttatcgtttt
    13021 tatggcaatt gccaatgttc attttgatga atatctattg gtcagaaaaa atttattgat
    13081 aagttctaaa tcgattaaac cggaatctct cgacgatatc cttggcgata ttctgaaaaa
    13141 agagacaacg ataacctctt ttttaaatat gccgacgtta tcattgagcc gaaccgaatc
    13201 gagtatgttg cgaatgtgga tggcaggtca gggaacgatt caaatctctg accaaatgaa
    13261 tatcaaagcc aagaccgttt catcgcataa aggtaatatt aaacgtaaga tcaaaacgca
    13321 taataaacag gttatctacc atgtcgtccg actgacggat aatgtgacta atggtatttt
    13381 tgtcaacatg cgctaacaca ttctgactgg tggtttccca ccagtcaggc tgaataagat
    13441 tactctgctt tctccacaaa gataccgtcc tgatgccctg cttcattaaa gaaccagatg
    13501 ccgagcgggt agtcttccag cgaaaccagg tacattgtgc cttcactaaa ctcctcaact
    13561 gccagtacca cgccaggacg acgcggaccg ccatccgttt tgactgttac ccgatcattc
    13621 accttcatcg ttttcctcct gtggctttgt gccagtgtag aacaatttcg tttttctggc
    13681 agcgccaggc gcgcgcgagt gctgattttc tcgacggtct atacttaaga gatgccaggc
    13741 ggacttaacg actggcggca acaacagagt aacggttgcg aggaaagatg atgaaaaccg
    13801 caaaagagta cagcgatacc gcaaaacgtg aggtcagcgt cgatgtcgat gccctgctgg
    13861 cggcgatcaa tgaaattagc gaaagcgaag ttcatcgcag ccagaacgat tctgaacacg
    13921 ttagcgtcga tggacgtgaa tatcatacat ggcgtgaatt ggcggatgcc ttcgaactgg
    13981 atattcatga cttcagcgtc tctgaagtga atcgttgaat gcacaataaa aaaatcccga
    14041 ccctgagggg gtcgggatga aacttgctta agcaagaagc acttaaaaaa ttcgttacac
    14101 caggaaatct gatgtgttca tcaccttatc cgcaattttt ttcgctgaca agaaaatatt
    14161 cgcgatatga atgattaacg ctacttatga atagaaatgt gacgtgcgtc accccttggt
    14221 ggctctggcg tcaacgggat cgctcccgaa aaaatcatct ttgattattt gtgctgtttt
    14281 gagaatcatc ctggtaaggg gagaactgcg cgaccatgtt ttcaattcaa caaccactac
    14341 tggtttttag cgatcttgat ggcaccctgc tggacagtca tagttatgac tggcaaccgg
    14401 ctgccccctg gctcagccgt ttacgcgaag cgaatgttcc cgtcattctc tgtagcagta
    14461 aaacttcagc ggaaatgctg tacttgcaaa aaacgttggg gctacaaggg ttaccactga
    14521 ttgccgaaaa tggcgcagtg atccagcttg ctgaacaatg gcaggatata gacggttttc
    14581 cacgcatcat ctcaggtatt agccatggcg aaatcagcca ggttttaaat acgctacgcg
    14641 agaaagaaca ttttaaattc acgactttcg atgatgtcga cgatgcaacc atcgccgaat
    14701 ggacgggatt aagccgtagc caggcggcgc tgacgcagct gcatgaggcg tcggtaacgc
    14761 taatctggcg cgacagtgac gagcgtatgg cacaatttac cgctcgtctg aacgaactgg
    14821 gcttacagtt tatgcagggt gcgcgcttct ggcacgtact ggatgcctct gccggaaaag
    14881 atcaggctgc caactggatt atcgcgacct atcaacaatt gtcaggcaaa cgcccaacca
    14941 cacttggcct gggcgatggg ccaaacgatg cgcccttact ggaggtaatg gattacgcgg
    15001 tgattgtgaa agggctaaac cgtgaagggg tgcatctgca tgatgaggat ccggcccgcg
    15061 tctggcgaac gcagcgtgaa ggaccggaag gctggggtga agggctggac cattttttct
    15121 ccgcccgtta agcgttatcg ctcgcgcata cccgattacg cccggcctgt ttagcgagat
    15181 aaagtcgacg gtcagccagt gactggagtt gttcaaaatc ataatcaccg gtttcctcgc
    15241 tgctacttac ccccagcgag gcactgatgc gtatcgtcgt actcttagca atcaacatct
    15301 ctttttcatt taacttcagg cgaatacgtt ctgcgacttc cgcagcctcc gtcagactcg
    15361 cgcctggcag aatcacacaa aactcctcac caccgacccg cccggcaacg tcctgcgcac
    15421 gcaaggaact gctaattaat ccggcagcat gagaaagaac acggtcgccc gcctgatgac
    15481 caaagcggtc attaatcgct ttaaaatggt caagatcgac ctgaatgaca gaaaaaggat
    15541 gttggtgcgt ctgatacaat ttagcgagcg gacgggcttt ttcgaacagt gcgccacggt
    15601 tatataaacg cgttaaggtg tcgtgccacg cctgccactg caacgagctt tgcagaacat
    15661 acatgttgct aaccatccgg cgaatcacat accaggagat gagtaacatg gtggtaaaga
    15721 gcgcccacag cagggttaat gcaatgctga tactgccgaa atcgccgcgc acgccttcgc
    15781 ttaacgtatg gacgcgcacc agcacaccgt cgaaatggtc cagacgttcc cagctaacat
    15841 agcgactgtc catacgaatg ccgccccgcg tgtcatgttc catcgcctgc gccagcaagg
    15901 ccagttcacg aggatcaaaa atattccctg ttggatggtc aggattggaa gaggtcaaaa
    15961 atctcagctt actgtcatag agctgatact caccatcgag gtttttatcg atggcgtttc
    16021 ttaaaaattg ctgcatggta cgcacgggaa tactcatccc cagcacgcca taccagtagt
    16081 tattactgtc tactggaaca ctgacggtaa cctgcggttc agtattgctg gcgtgttccg
    16141 gttgcgaagt aaaccagcgt accgcgcggt gacgattttc tcgttgcgaa tggccgataa
    16201 accagggttg ggtgacatag ccgtaataac gcgttggtac attgcgcgta aacaaggtcg
    16261 gctgcgtcga aacgtaaaat ccggcacgcg agacatacat cgcctgttca accatcgacg
    16321 aggtgttgtg cgccagtcgc agcaagtaac caacttccag tgcagcggta atttcattgt
    16381 cgaggctttc attttcgcga gacaggagat tcccctcgct gactaatgca tccgatacgc
    16441 cattgactga cagggtgcgt cgtcggttga gttcgatttt ccaggcgtgc tcgtcgcgat
    16501 gctgttcgaa ctcggttaca gcatcacgca gagaggtgaa atccagtggc gctacgagag
    16561 cttcgcgcat gccattacgc aaaaagatca gtttgtcgac gttatactgc aaatgcttat
    16621 cgagcgcgtt ggctacgttt tccagatgat tacgctggct ggagatatag gcatcttcca
    16681 gcaccactac ttcacgccag gtgagcaagg tggaaaaaag caataccacg ataaagcaga
    16741 gattaacgac atgaccaggc cccaggcggc gtgcgagttt tttcaaccag ctctggtttt
    16801 ccatttttgt ctcgtgctgc accctgacga ctcccaacct gcttttttat gattctggca
    16861 taacctggcg atagcgtacc gcaaaacagc gataatttta taatggtggc atgataagac
    16921 gcgacagaca tcgatagttt tataacgtag gcatgataag acgcgatagc gtcgcatcag
    16981 gcaattcagt gcagacactg gcacacccga ccggatgcga cgtgagcgcc acatccggtc
    17041 gggtgttaat gatacccttt caaacttctt ccgcaggacg acttccctcc cctggaacaa
    17101 gctcggtttc atgaaaagct tcccgcttga ccccgtaacc gtcataccaa cgacactcaa
    17161 ccataccgct ggagtatccg gtgacaatca tccgcgggcc gccctcttta accgtaactt
    17221 cctcactaac cataaagctc atactcgcct ccttttttcg agtgaaactt gttcacctta
    17281 gttgaagatg gcgaattttg cataatagcc agtgcgagta ttaatgtgcc tgaccgcgta
    17341 ttttcgctac ggctttcact cccagcacca cgatgccgcc gatgataaat ccaagaatca
    17401 gatttaaaac agtcggtaat atcatcgcca ccactgcact ttgctgcccg gcgaaatgtt
    17461 caatggcgtg atgcagcggc gcaataccat gtaccacaat cccgccgccg acgaggaaca
    17521 tcgccagcgt gccgacaatc gataacgcct tcatcaacca gggcgcaatg atcaataacc
    17581 ctttacctaa tgcctgcatc agcgcgctgg atttttccgc cagccaatac cccaggtcat
    17641 caatcttaac gataacccct accagaccgt agacgcccac ggtcactacc agcgcgatgc
    17701 ctgaaagcac cagcacctga ttaagcaacg gcgcttcggc cacaatcccc agcgtgatgg
    17761 cgacgatttc ggcagacaat ataaaatcgg tacgaatcgc cccttttact ttgtcctttt
    17821 caaacttcag cggatcctgc gccgccagct tctccagacg ctgctggctc tgcgccggat
    17881 cttctttatg tttacgcgcc tccagcatat gcagcacttt ctctactcct tcaaagcaga
    17941 gaaacgcgcc accaatcatc aacagaggcg taatcgccca cgggataaac gcactgatga
    18001 tcagcgccag cggcaccaga atcactttat taatcagcga tcctttcgcc acgccccaga
    18061 ccacgggaag ttcccggttg gcccgcacgc ctgaaacttg ttgcgcattg agcgataagt
    18121 catcccctaa tacaccggcg gttttcttcg ccgccagttt gcccatcacg gagatatcgt
    18181 ccagtagtgt ggcgatatca tcgagcaacg tcagtaaact actcccggcc aaaagcattc
    18241 cctctcattt ttattgttga agggtgtaag tatgaagcaa aaacggcagc tctgatactt
    18301 gcttcacagg tcaacatttt tttaacaatt aagtgcaatt atatctctcg ccagcagaat
    18361 gattttcacg tttactatga accacctttt tatttccacc gtgagggatt atgcgtttcc
    18421 gccagttgtt accgcttttt ggcgcgctgt ttgcgttgta tatcatttgg ggctcaacct
    18481 attttgtcat tcggattggc gtggaaagct ggcctccgtt aatgatggca ggcgttcgat
    18541 tcctggcagc cggtatttta ttgatggcat ttttgctact gcgcggacac aagctccccc
    18601 cgctacgtcc gctgctcaat gccgcgctga ttggcctgtt attgctggct gtcggtaatg
    18661 gcatggtgac ggttgccgag catcaaaatg ttccttccgg catcgccgcc gttgtggttg
    18721 caaccgtgcc cctctttacc ctgtgcttca gccgcctgtt tggcattaaa acgcgcaaac
    18781 tggaatgggt gggtatcgcc attgggcttg ccggaatcat catgctcaat agcggtggaa
    18841 atttaagcgg caatccgtgg ggcgcgattc tgattttaat cggctcgatt agctgggcgt
    18901 ttggctcggt ttatggctcg cgcattacct tacctgtagg gatgatggcg ggtgctattg
    18961 agatgctggc ggcaggcgtg gtgttaatga tcgcgtcgat gattgcaggt gaaaaactga
    19021 cggcgctccc ttccctttca ggcttccttg cggtcggcta tctggcgctg tttggttcga
    19081 ttatcgccat caacgcttat atgtatttaa tccgtaatgt cagtccggct ctcgccacca
    19141 gctacgccta cgttaacccg gtggtcgcgg tcttgctggg tacgggactg ggcggagaga
    19201 cgctgtcgaa gattgaatgg ctggcgctcg gcgtaattgt cttcgcggtg gtgctggtca
    19261 cgttgggaaa atatctcttt ccggcaaaac ccgtagttgc gccagttatt caggacgcat
    19321 caagcgagta aatgaatccc ctgcgtgtcg atctgcgcgc tggcaccttc gccgcagatc
    19381 cactcttcca gacgctcgct aagcgcttca tccgtcagct tctcgcgccc acgtaacgcg
    19441 cactcccaga caatcaatac gcgccagccg agttcctgca agcgactgat atcgcggcga
    19501 tcgcgctcaa catttttacc tatcttctcc agccagaact cggttcgagt cgcaggcact
    19561 ttaaacagat agcagtgatg atgatgccag aagcagccgt gggtaaatat cacgcagcga
    19621 tattcatcaa cgacaaaatc cgggcgtccg ggcagactgg cgtcctgaac gcgaaacgcc
    19681 aggccctgcc cggttaacag actggcgagg cgcttctcta tcgccgtatc acgcgtggca
    19741 atcgcgcgca tatttttgct gcgagtggcc ttatcgtgaa cgtcggccat gttgtgcctc
    19801 ttgctgacgc aacgccaccg cctgtttgat ttttggctca agcagttttg ccaccgcggc
    19861 aaagactggc acgaccaccg agttaccgaa ctggcgatag gcctgagtgt ccgaaaccgg
    19921 aatgcgaaat ttcgcttctc ccggcgcttc aaaacccatt aagcgcgcgc attcacgagg
    19981 cgttaaccga cgtggacgat gttgctgatt ctgcggatcg tcaaagtctt tctcaccttt
    20041 ggccatatcc cagccgcgat cgattaaaat ttccgcgcca tctttgtaat aacgcgcaga
    20101 cagcgtacgc gtgacgcttt gcggattgtt cggataaacc attccataac cgaagccgtt
    20161 accgcgcgcc tgatgttttt tcgcatatcg atagaggtac ttccacagca ccggcgtcag
    20221 gatatatttc gcctcgacca tcgggtccag cagctgcgcc agcgtcactc gctgcgcagg
    20281 gaaacattcg ctgatatcac gcagggtaaa atcggctttc agattcagat cgcgacgaaa
    20341 acccaccagc acgatgcgtt cacggtgctg cggcagaaaa tgtttgccgt cgatgatttt
    20401 cggatcgtct ggcccgttat cttctgcatc agccacgtca tagcccagtt cgtccagcgt
    20461 ctgcatgatg atgcggaacg ttttaccctg gtcgtgactt ttcaggtttt tgacgttttc
    20521 gagcacaaac atcgccggac gacgcgcgtc gataatgcgc accacatcaa agaacagcgt
    20581 gccctgagta tcgcaggcaa aaccgtgcgc ccgcccgagc gagttctttt tcgatacgcc
    20641 agccagcgaa aatggctgac aagggaaacc ggccagtaaa acatcgtgtt caggaatgtg
    20701 ttgacgaata tgttccgccg ccgcctcatc actcacgcct tctttatggc tgagggtgat
    20761 gtcgcggata tcttcattaa aatgatgcgt cgccggatcg caataatggt tggctttata
    20821 agtgcgtacc gcatgtttgt tccattcgct ggtaaacacg cactgtccgc caatcgattc
    20881 aaaaccgcga cggatgccgc caattccggc gaatagatcg ataaagcgaa acgcataatg
    20941 cggatgatgt gccggtggtt tgggtaataa cgtttgcaga tgggcgaact ctttctcact
    21001 taaacggtgc catgccgagt cattcgccag cgcacgtttt aaaattgccg cgctccagtg
    21061 attctcacct acaccattaa gctgcgccac cagcgtttta acatcataaa tttgcagcag
    21121 tttctccagc attgcctgtg cggcattccc ggtgctgtat gaatcggtta ctgatatatt
    21181 ttcctgcata gatttcaccg gccatctgag atggccgaca ggttaacata attacagcca
    21241 tttagggcca cgcgctcagc cagcggatga aaacgcatct atcaccttgt gatcgacacc
    21301 ttcattctcc cccgccagtt cggcactgag cttcgccata aactcgacta aaaaatgcgc
    21361 gttgtgctgc gccagctgct tgcctcttgc ggtttgcatg gtttgcggta gcttcagcaa
    21421 cttagtctgg aaatgatcca gcgcgtagcg tttatcatca agcgggcgat gctgtgcgaa
    21481 cggatcttca ccatcaaaca gcgccacgcc caatgcccct gaaacggcaa acacgcgcgc
    21541 caggccaatc gcccccaggg cttccagccg atccgcatcc tggacgattt tagcttccgt
    21601 tgttaagggg gcgatttgcg cgctgaaact gtgagcggca atggcatgac aaacggcctc
    21661 gattttctcc gccggaaatt gctcgaactc ttcacggagc aggcgacgcg tctcttctgc
    21721 cgccaggatt gaagaacgct gcctttgcgg atgatttttc gccaggctca caatatcgtg
    21781 aaaataacag gcggttaaaa tcaccagcat atcgacatcg tcatctgccg ccagcttctg
    21841 tgccgtagcc cagacgcggc gaaaatgaca cacgtcatgg gccgcgtcct ggtgctgatg
    21901 atggtttttc aaccagtttt cgaactgtgc ctgccagtgt tgtaagtcca tacgcgctcc
    21961 gttgccaaag agtcgccacc ttagcagttt ttattcatag cgtctgctac gtgcgggacg
    22021 aggtcgggtg taccaggcaa tgctgcctaa caatgccccg accagagcca taaataaaaa
    22081 tccgaccagt gcccctaacc actttcccgc ggtagagcct aaatcgcttt caaatcccga
    22141 cacgggggca tcggggatcc ggctcatcac ccagatgtaa cgcgccattg cccagacgaa
    22201 atagccacac catgtataga aagcccacaa agccagtttg ccgccagggc tgcgagagag
    22261 tctctcttcc atctttgtcg aaccgtattc cattttgaga taacccggaa aattcagact
    22321 tagtactatt atgtgataaa agtcacattt ttccacattg gatgagccag tcaagactca
    22381 ctcatttttt gaacaaatag ataatttttg cgcaacggca gtgaatacag gaaaaatcgt
    22441 tttgagcgat tttatttatc tttgagatcg gctttattaa ttcatttaat caatatatta
    22501 gcactgaata caattatacc tttttatttt tctgtgtcat gatgcctccg ttattagcct
    22561 tttatcgtct tgtttatatt ttttgggccg gcatgatgcc ggc
//
LOCUS       DAZFQD010000078        22294 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.078, whole genome shotgun sequence.
ACCESSION   DAZFQD010000078 DAZFQD010000000
VERSION     DAZFQD010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22294
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.078"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            217..351
                     /locus_tag="ACHKTW_003029"
                     /pseudo
     CDS             217..351
                     /locus_tag="ACHKTW_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958721.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
     gene            472..1212
                     /locus_tag="ACHKTW_003030"
     CDS             472..1212
                     /locus_tag="ACHKTW_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO6464009.1"
                     /translation="MNNVIPLQNSPERVSLLPIAPGVDFATALSLRRMATSTGATPAY
                     LLAPEVSALLFYMPDQRHHMLFATLWNTGMRIGEARMLTPESFDLDGVRPFVRILSEK
                     VRARRGRPPKDEVRLVPLTDISYVRQMESWMITTRPRRREPLWAVTDETMRNWLKQAV
                     RRAEADGVHFSIPVTPHTFRHSYIMHMLYHRQPRKVIQALAGHRDPRSMEVYTRVFAL
                     DMAATLAVPFTGDGRDAAEILRTLPPLK"
     gene            complement(1491..2468)
                     /locus_tag="ACHKTW_003031"
     CDS             complement(1491..2468)
                     /locus_tag="ACHKTW_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFO6464010.1"
                     /translation="MDKSSGELVTLTPNNNNTVQPVALMRLGVFVPTLKSLKNSKKNT
                     LSRTDATEELTRLSLARAEGFDKVEITGPRLDMDNDFKTWVGIIHSFARHNVIGDKVE
                     LPFVEFAKLCGIPSSQSSRRLRERISPSLKRIAGTVISFSRTDEKHTREYITHLVQSA
                     YYDTERDIVQLQADPRLFELYQFDRKVLLQLKAINALKRRESAQALYTFIESLPRDPA
                     PISLARLRARLNLKSPVFSQNQTVRRAMEQLREIGYLDYTEIQRGRTKLFCIHYRRPR
                     LKAPNDESKENPLPPSPAEKVSPEMAEKLALLEKLGITLDDLEKLFKSR"
     gene            complement(3830..5043)
                     /locus_tag="ACHKTW_003032"
     CDS             complement(join(3830..4752,4752..5043))
                     /locus_tag="ACHKTW_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558238.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFO6464011.1"
                     /translation="MTKNTRFSPEVRQRAVRMVLESQGEYDSQWAAICSIAPKIGCTP
                     ETLRVWVRQHERDTGGGDGGLTSAERQRLKELERENRELRCSNDILRQASAYFGEGGV
                     RPPLEKMMPLLDKLREQYGVRPVCSELHIAPSTYYHCQQQRHHPDKRSARAQHDDWLK
                     KEIQRVYDENHQVYGVRKVWRQLLREGIRVARCTVARLMAVMGLAGVLRGKKVRTTVS
                     RKAVAACDRVNRQFVAERPDQLWVADFTWVSTWQGFVYVAFIIDVFAGYIVGWQVSSS
                     METTFVLDALEQALWARRPSGTIHHSDKGSQYVSLAYTQRLKEAGLLASTGSTGDSYD
                     NAMAESINGLYKAEVIHRKSWKNRAEVELATLTWVDWYNNRRLLERLGHTPPAEAEKA
                     YYASIGNNDLAA"
     misc_feature    complement(4646..4762)
                     /locus_tag="ACHKTW_003032"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01497"
     gene            5232..5816
                     /locus_tag="ACHKTW_003033"
     CDS             5232..5816
                     /locus_tag="ACHKTW_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6464012.1"
                     /translation="MGKMKELLFEIQEERTDEWIAENYTDAEEGTPEWDAAAQEYSWF
                     QDWMEEAAEQQYFEASLASIPDRLQDAKDELFELENLMQFNQPGIVERMAYVHCVSVL
                     DSFLMYSARALLNHPPHLQRFLQVADSLIANKEDRRKLRASKWVEQEPDTATPEKVYT
                     WRAQSLVAKKTFQSHKVIDRYFSTMLATPHEWPL"
     gene            6059..6580
                     /locus_tag="ACHKTW_003034"
                     /pseudo
     CDS             6059..6580
                     /locus_tag="ACHKTW_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001495932.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            6761..7405
                     /locus_tag="ACHKTW_003035"
     CDS             6761..7405
                     /locus_tag="ACHKTW_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001144037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFO6464013.1"
                     /translation="MPVIANTHPKGGVGKTTSSVNIVGEMKSDTVDLDTHTGLSIILG
                     LRPEGKEISVKVPKTVDELIEIMTPYKNSDKTLLIDCGGFDSDLTRTAIAFADCVIVP
                     SKDSLTERIGLMHFDGVLDEISSIMGTDITAHLYLCKVNPNKKKFPKLDAILPSFKHL
                     KLMKSRISARAEFEDVIETGMGITESVHGRYSAGGKEVIALIEEINHLIENNKQ"
     gene            7492..7800
                     /locus_tag="ACHKTW_003036"
     CDS             7492..7800
                     /locus_tag="ACHKTW_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000030202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone GroEL"
                     /protein_id="HFO6464014.1"
                     /translation="MSKAKFSAQAEKLKNLVDSARLQTSSSAVTATVPSQPNEGTEEV
                     SGRPVAARTKAKNLKAIPLSYFEAHAELKATSKTSLDFSSYIIEAIREKLERDGAIGQ
                     "
     gene            8214..9194
                     /locus_tag="ACHKTW_003037"
     CDS             8214..9194
                     /locus_tag="ACHKTW_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HFO6464015.1"
                     /translation="MKIFIDDGSTNIKLAWLEDGDVKTLISPNSFKPEWSFSLLDDAA
                     PANYEIDGEKFSFDPLSADAVVTTETRYQYSDVNVVAIQHALQQTGLKAQPIDVIVTL
                     PISEYLDANNQKNKQNIERKKKNVMREVRVQGSDAFVIRSVSVLPESIPAGFSVLAGL
                     EDDESLLIVDLGGTTLDVSHVRSKMTGITKTWCDPNIGVSLITSGVKEQMAVHANTRV
                     SSFQADNIIVHRNEPDYLSRRIYNAEQRESIINVINERQKLLIKRVNDVISRFTDYTH
                     VMCVGGGAEIVAEAVKNLTKVPDERFYLSSSPQFDLVMGMIKMKGGVTNE"
     gene            9187..9603
                     /locus_tag="ACHKTW_003038"
     CDS             9187..9603
                     /locus_tag="ACHKTW_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HFO6464016.1"
                     /translation="MSDENKSRRCSFELFPDERTGDKIADELIANEKLKERGRFMRAM
                     LVTGAAFAAIDKRLPLLISELLTENTTLDDINKVISSVIPGAFSVEKKLLELLEKQSG
                     LHTSVDCSTPLTEQSLSRNDGEDQTRRNAENMFGDD"
     gene            complement(9605..10572)
                     /gene="umuC"
                     /locus_tag="ACHKTW_003039"
                     /pseudo
     CDS             complement(9605..10572)
                     /gene="umuC"
                     /locus_tag="ACHKTW_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566943.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V subunit
                     UmuC"
     gene            complement(10562..10981)
                     /gene="umuD"
                     /locus_tag="ACHKTW_003040"
     CDS             complement(10562..10981)
                     /gene="umuD"
                     /locus_tag="ACHKTW_003040"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000109083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HFO6464017.1"
                     /translation="MSTVYHRPADPSGNDSYVRPLFADRCQAGFPSPATDYAEQELDL
                     NSYCISRPAATFFLRASGESMNQAGVQNGDLLVVDRAEKPQHGDIVIAEIDGEFTVKR
                     LLLRPRPALEPVSDSPEFRTLYPENICIFGVVTHVVH"
     gene            complement(10978..11226)
                     /locus_tag="ACHKTW_003041"
     CDS             complement(10978..11226)
                     /locus_tag="ACHKTW_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFO6464018.1"
                     /translation="MIRIEILFDRQSTKNLKSGTLQALQNEIERRLKPHYPEIWLRID
                     QGSAPSVSVTGARNDKDKERILSLLEEIWQDDSWLPAA"
     gene            12249..13973
                     /gene="ltrA"
                     /locus_tag="ACHKTW_003042"
     CDS             12249..13973
                     /gene="ltrA"
                     /locus_tag="ACHKTW_003042"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFO6464019.1"
                     /translation="MTEQATTCKGASLLNGDSWHSINWRQCYREVRRLQARIVKATRE
                     GKHGKVKSLQWILTHSFSGRAVAVRRVTENSGKRTPGVDGQTWSSPEVKFLAINLLKR
                     RGYKPQPLKRVYIPKSNGKSRPLGIPTMKDRAMQALYLLALEPVAEVTADQRSFGFRT
                     GRSTADAIAQCFCVLAQKTSAEWVLEGDIRGCFDNISHQWLIDNTSTDRQILTKWLKA
                     GYREKGQLFPVNSGTPQGGIISPVLANIALDGLEALLASEFKKRTVKGRLVNPKVNYV
                     RYADDFIITGESKELLESQVLPVVRRFMAERGLMLSPEKTKITHIEEGFDFLGQNIRK
                     YGGKMLIKPSKANVSSFLKKIRAVIKGNKAMDQLTLIRMLNPMIKGWAAYHQHIVAKV
                     AFNKVDNEIWLALWRWAVRRHPNKGKKWIRKRYFHQQGARNWSFSTATGELLANGKPQ
                     YANLRKAIDTPINRFKPIKIAANPFDPQWEMYFEERCADKMRHKLQGRKKLIQIWFEQ
                     RGRCPICDERITSDSQWQVHHIIRRVDGGSNCLSNLIMLHPMCHTLVHAKGIHVVKPA
                     HESGLRKA"
     gene            14050..14952
                     /locus_tag="ACHKTW_003043"
     CDS             14050..14952
                     /locus_tag="ACHKTW_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HFO6464020.1"
                     /translation="MHFSGEPAQIAEIKRLASGAVTPFYRRATNEGIQLFLAGSAGLL
                     QITENIRSEQCPGVTAAGRGAVSTENIAFTRWLTHLQNGVLLDEQNCLMLHELWLQSG
                     TGQRRWEGLPDDVRETITVHFTAKRGDWCDIWGNEDVSVWWNRLCDNVVPEKTMPFDL
                     LTVLPTRLDVEVNGFNGGVLNGVPSAYHWYTERYGVKWPCGYDLNISSQGENFIQVDF
                     DTPWCQPESDVVAELSRRFGCTLEHWYAEQGCNFCGWQRYERGELVDVLWGELEWSSP
                     TDDDELPEVTGPAWIVDKVAHYGG"
     gene            14949..15260
                     /locus_tag="ACHKTW_003044"
     CDS             14949..15260
                     /locus_tag="ACHKTW_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6464021.1"
                     /translation="MKYDGDGRATARFFSGKGCRHAPLFTAPADAARHKRCLWSVSRV
                     RRARDGRFYRSRLASVTVYASLSPFSDERPSSRFRGITLPSNRRRLRYSTVGLTRYRT
                     R"
     gene            15336..16019
                     /locus_tag="ACHKTW_003045"
     CDS             15336..16019
                     /locus_tag="ACHKTW_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HFO6464022.1"
                     /translation="MSRFVLGNCIDVIARIPDNAIDFILTDPPYLVGFRDRSGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWNRVDRFVAAWKNAGFSVVGHLVFTKNYT
                     SKAAYVGYRHECAYILAKGRPRLPQNPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLSAVQRAMQQGAAN
                     DDWFMPEAA"
     gene            16020..16241
                     /locus_tag="ACHKTW_003046"
     CDS             16020..16241
                     /locus_tag="ACHKTW_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6464023.1"
                     /translation="MHYAGHEKLRAEVAEVANAMCDLRTTMNEMERRYSFNADTLPER
                     LVRQTLFRANRLLMEAYTEILELDACFKD"
     gene            16222..16689
                     /locus_tag="ACHKTW_003047"
     CDS             16222..16689
                     /locus_tag="ACHKTW_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HFO6464024.1"
                     /translation="MRALKIEKETRMYGTCETLCRELAAKYPGDTPLMLVVWSPEEIQ
                     ALADGMDISLSDHEIRTVLARLEDIPEDQRIESGISSAAAMEIISNVSENRQVTVPAE
                     LLASLIQTAEQALWKREWAARDYGLAVPECVTRRQAVVNQARILLKNNTHEND"
     gene            16734..17504
                     /locus_tag="ACHKTW_003048"
     CDS             16734..17504
                     /locus_tag="ACHKTW_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001005031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6464025.1"
                     /translation="MNKTLNALICRHARDLLLAQGWPEETDVDQRNLKYPGWISIYVR
                     LDAPRLATLLVNRHDGVLPPHLASAIQKLTGTGAELVLSGSQWQSLPVLPADGTQVSF
                     PYAGEWLTEDEIRAVLDAVRNAVRSICYQVADDARRIRAALTTTGQTLLTRQTRRFRL
                     VVKESDYPCWLDEDDENLPVVLDAILNRGARFSSVEMYLVCECVEHILASGLVCDVLR
                     IPDEPSRRWFDRDILREVVLEARDEIRSMADALAKIRG"
     gene            17918..18343
                     /locus_tag="ACHKTW_003049"
     CDS             17918..18343
                     /locus_tag="ACHKTW_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFO6464026.1"
                     /translation="MQYAKPVTLNVEECDRLSFLPYLFGLDFLYAEASVYALAKKMMP
                     EYEGGFWHFIRLPDGGGYMMPDGDRFHLVNGENWFDRTVSADAAGIILTSLVINRQLW
                     LYHDSGDAGLTQLYRMRDAQLWSHIEFHPECNAIYAALD"
     gene            18343..18834
                     /locus_tag="ACHKTW_003050"
     CDS             18343..18834
                     /locus_tag="ACHKTW_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HFO6464027.1"
                     /translation="MNGRGGNAAPVKKTRTCTVKEIIRDVLDTDVPDSECVLAVVLTR
                     GDVRHMAQDWNLSDDELETVMQRLDDAFEYGADVSIVHDVVRELMEEKRASRQVTVPA
                     VMLEKVMALAGSEMKRLYAVGSENGGDGDAFVREEREAMDVVLQGQAVEAVCQPARPP
                     LAG"
     gene            complement(18947..19220)
                     /locus_tag="ACHKTW_003051"
                     /pseudo
     CDS             complement(18947..19220)
                     /locus_tag="ACHKTW_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052655.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            19755..19985
                     /locus_tag="ACHKTW_003052"
     CDS             19755..19985
                     /locus_tag="ACHKTW_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6464028.1"
                     /translation="MTVSIVSPSAAAVKPRRHPRFRREDIPAPEIDPVLKAFGRHIAR
                     SFHRGRGVHIPAMKNTAFGQVLRTLELKRAFN"
     gene            20037..21398
                     /locus_tag="ACHKTW_003053"
     CDS             20037..21398
                     /locus_tag="ACHKTW_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3560 domain-containing protein"
                     /protein_id="HFO6464029.1"
                     /translation="MTSAAITMTAPEAASPVQMYRATYSPDDNKLRLYAVSRLDPETY
                     KKVHDAGFRWAPKQALFVAPAWTPGREDVLLSLAGEIEDEDSTLAERQEARAERFTGY
                     SGKRASESAQALDEVERLAAMIPPGQPILVGHHSERRARRDAQRIENGMKRAVMLFER
                     AEYWEERARSALLHAKYKERPDVRWRRIKKIEADLRKAEKTIAQSQKYLTMWRAESLD
                     LNMAKLISSHDHISACFPLDTYPRPAEKSQYEGSRSLWSALDDDIITTEQAREIAIRC
                     HERQIQHQQRWVNHYQNRLIYERAMLDESGGVVTRTQDFEPGGQVFSRGEWLTIIRVN
                     KSNGAVSSVTTPNYSFLGYSGTMKVTPDRITDYKAPSAEEAAVASQAAKRPPVVNYPG
                     EGFREMTKAQWAALPRDCKAVRSVAEAEDHGAYRYRRTMDNNFRLVNVYITDMKITEI
                     PQK"
     gene            21446..22009
                     /locus_tag="ACHKTW_003054"
     CDS             21446..22009
                     /locus_tag="ACHKTW_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HFO6464030.1"
                     /translation="MHSQLRERIRLMRARLDNAAPVAEIRAESQLFVTPAPVCDRLVM
                     LAEISNRDHILEPSAGTGAILRAIRDTAPGAMCDAVEINSGLVRYLRENFNGVRVQCG
                     DFMEWQPVQYYSRVIMNPPFSHGQDIRHILRAFSLLRPGGVLVAVCLNGPRQQEKLLP
                     FSDVREELPRGTFAYTDVPTMIIRLRA"
     gene            complement(22077..>22294)
                     /locus_tag="ACHKTW_003055"
     CDS             complement(22077..>22294)
                     /locus_tag="ACHKTW_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6464031.1"
                     /translation="RCARQTGQHCSSQNQSSRKKKNSSSSSSSSSSSSSSSSSSRNNN
                     IISNRQMTGMKKQKPPSERPRNERGGA"
BASE COUNT         5306 a         5567 c         5969 g         5452 t
ORIGIN      
        1 aatgtagtaa ttatataaat cttatcgaaa gtgattttct gcataatcat tattatgatt
       61 tatattaatc atggacagga ttgcatataa atcagtcatt atgctgagtc tgcaaccttc
      121 ctgtgtaaag tcaaactgtc agggggacgg tgacgtcccg ggccagaata cacgtggtgc
      181 accttctccc tttctgttcc ctttctctgt tttctccgat cccattcatc ctggcattac
      241 cccaattctc ccaaaaacgg taaaccagcc ctcagcccgc gccacgactg gcttaatacg
      301 ggatccctgc tgcaaaacgt acctttacgt atttggtttt acccgcaata atgcgtgata
      361 agcaaataaa aggatccgcg cgctgcgatt tattgtcgtg gaaggatccg ggcgttcagg
      421 tcagaatacc tgtatccata aacagtgcgt gtgctacgtg aaaaataact catgaacaat
      481 gtcattcccc tgcagaattc accagaacgc gtctccctgt tacccattgc gccgggggtg
      541 gattttgcaa cagcgctctc cctgagaaga atggccactt ccacaggggc cacaccggcc
      601 tacctgctgg ccccggaagt gagtgccctt cttttttata tgccggatca gcgtcaccat
      661 atgctgttcg ccaccctctg gaataccgga atgcgtattg gcgaagcccg gatgctgaca
      721 ccggaatcat ttgacctgga tggagtaaga ccgtttgtgc ggatcctgtc cgaaaaagtg
      781 cgtgcgcgac gcggacgccc gccaaaagat gaagtccgcc tggttccgct gacggatata
      841 agctatgtca ggcagatgga aagctggatg atcaccaccc ggccccgtcg tcgtgaacca
      901 ttatgggccg tgaccgacga aaccatgcgc aactggctga agcaggctgt cagacgggcc
      961 gaagctgacg gagtacactt ttcgattccg gtcacaccac acaccttccg gcacagctat
     1021 atcatgcaca tgctctatca ccgccagccc cggaaagtca tccaggcact ggctggtcac
     1081 agggatccac gttctatgga ggtttatacc agagtgtttg cactggatat ggctgccacg
     1141 ctggcagtgc ctttcacagg tgacgggcgg gatgctgcgg agatcctgcg tacactgcct
     1201 cccctgaagt aacggcactt ccttatataa gcagtggtca gcagaacaga cataacataa
     1261 gctggagcag gtagataagc tgtagtgagt aaaccatgtt ttatgaaggg agcaatgcct
     1321 cagcatcagc ttacggggtg actcacgtaa gggacaggca gatggcagct cagccacagg
     1381 cagcactgca tgaaactgaa tataaactgc agtgagcaga ccactcactg cacctgataa
     1441 tataagctgt agtcagtaaa ggagcactct tcactgacta cagtttatgt tcagcgggat
     1501 ttgaagagtt tttccaggtc atccagcgtg atgcccagtt tttcaagcag ggcaagcttc
     1561 tccgccatct ccggactgac tttttccgca ggtgaaggtg gcaacggatt ttccttactc
     1621 tcatcattcg gtgcttttaa ccggggacgc cggtagtgaa tgcagaagag ttttgtccgc
     1681 ccccgctgga tctccgtgta atcaagatat ccaatctcgc gcagctgctc cattgcccgt
     1741 ctgaccgtct ggttctggga aaatacagga gacttcagat tgaggcgtgc acgcagccgc
     1801 gccagcgata tcggtgccgg atcccggggc aggctctcta taaaggtgta aagtgcctgg
     1861 gcggactccc gtcgcttcag ggcattaatc gccttaagct gaagaaggac ctttctgtca
     1921 aactggtaca gttcaaaaag gcggggatca gcctgtaact gaacaatatc ccgttcagta
     1981 tcgtagtagg ctgactgtac cagatgggtg atgtattccc gggtgtgctt ctcatcggta
     2041 cgggaaaacg aaatcacggt accggcaatg cgcttcaggg aagggctgat gcgctcacgc
     2101 agcctgcggg atgactggct tgaaggtata ccacacagtt ttgcaaactc gacaaaaggc
     2161 agttcaactt tgtcaccaat tacgttatgg cgggcaaagg aatgaatgat ccccacccag
     2221 gtcttgaaat cgttatccat atccaggcgg gggccggtga tctcaacctt atcgaatccc
     2281 tcagcacggg ccagggaaag acgtgtcagc tcttccgtgg catcagtacg tgacagtgta
     2341 ttttttttac tgttcttcag tgatttaagg gtcggtacaa aaacgcccag acgcatcagc
     2401 gccacaggtt gtacggtgtt gttattgttt ggtgtcagtg tcaccagctc accggacgac
     2461 ttatccacct cgccgaacag ttttttgatg tctgaatttt cgttttccag aaagcctcct
     2521 gcctgtggac agtaaaagaa taaacagaaa atcgtgtgtc agaaccccgc taactgactg
     2581 aagaaaaatc tgcagcctgt atttaacagc tgagtctggc gtactgacca tagtttatgt
     2641 cattaactta ctgcagtata tgtattactt aatacagctt atcattaact tgttacagtt
     2701 tatcagtaac atgccacagc ttatgtgatc gtgctcccgg accattaatg acaaggtttg
     2761 cagcgatatg gggatcttta atggatcctt tgttgatcat tcatggatct gtttactgga
     2821 tcgagcctcc ggatatgtgg ataaatcatt ccggaaaaac aacacacact accgattatg
     2881 aaggcttcag ggcgtacctg ctcactgtga ttacagatcc attgcgcttc ctcgccggtt
     2941 cgcgggcttc gcccggtcac cggtctgcgg cgagcaccgt tttttctgaa gtctgtcttt
     3001 acaggtaatt ttcgactcaa tctgccgtac ttcctgaaca tacggtcata tccgaagtac
     3061 acttcatatg tttgtttcaa tggcaaaggt gcatacagac tgactccatg aatatcctga
     3121 caactgatat cacttaattt accgacagtc ttaagactaa tcgtcagagt cctgatagtt
     3181 acggatcttc cttttccccc acaacaacgc caccttctgt cagcacaaca tatagtgctt
     3241 aatttagatg ctaatgacac tatatattgt gtctccttca ttccctgtgg gcccgttcac
     3301 aacgacagaa aagggtgcaa gagccaagac gcaaccgtgt tacacttccg gaaagttgtt
     3361 ataagacaga aaatcggatc tcaccggaac ggccattcgt taatttaata atgtgtctat
     3421 atgtctgatt attatcagtg gagtgatgat tgcggtagga gaaagtcatt agagtgacag
     3481 atgtagaggt atgtgcagct gattatgctg cacatcataa ttaccattgg tgacttttta
     3541 ctaaggaact aaatgcgcgc atcaatagtt catgtctgga attatctgac acgttgtggt
     3601 cataaattcc tatggttaaa aataaaccct gtaccgccag gatataccgg ttcggtatat
     3661 acacatcagt ttgcaattta cagcgcgtcg ggtcgtattc tggccacgct tgatgtgcac
     3721 agacaacaat gggtgtgttc aggcttcact cccaactgtc tacccactga taaaaatccg
     3781 gtcatgctga accgccccgg gtttcctgga gagtgtttta tctgtgaact caggctgcca
     3841 gatcattatt tccgatggaa gcataataag ctttttctgc ttctgccgga ggagtatggc
     3901 ccagcctttc cagcaatcgt cgattgttat accagtccac ccacgttagt gtggccagtt
     3961 ccacttctgc acggtttttc cagctcttac ggtgtattac ctccgctttg taaagaccat
     4021 tgatgctctc cgccatcgcg ttgtcatacg agtcgcctgt acttcctgtt gatgccagta
     4081 atccggcttc cttaagccgc tgcgtgtagg ccagcgatac atactgagaa cctttatcac
     4141 tgtgatggat tgtgccggac ggccgacggg cccacaacgc ctgctccagt gcatccagca
     4201 cgaatgttgt ttccatggat gatgagacct gccatcccac gatgtatccg gcgaacacat
     4261 caatgatgaa cgccacataa acgaagccct gccatgtgct tacccaggta aaatcagcca
     4321 cccacaactg gtctggacgt tctgccacga actgacggtt tacgcggtcg catgcggcaa
     4381 cggctttccg gctgacggta gtgcggacct ttttaccccg gagaacaccg gcaagtccca
     4441 taactgccat gagacgtgcc acagtgcatc tggccactct gataccttcc cgtaacaact
     4501 gacgccagac tttacgcaca ccgtatacct ggtgattttc atcgtatacg cgctgtatct
     4561 ctttcttcag ccagtcatcg tgctgcgcac gggcactgcg tttatccgga tgatgtcgct
     4621 gttgctgaca atggtaatac gttgacgggg caatatgcag ttcgctgcat accggtctga
     4681 ccccgtactg ctcacgcagc ttatccagca gtggcatcat tttttccaga ggcggtcgaa
     4741 ctccgccttc gcaaaataag cggaagcctg gcgaaggata tcgttactgc agcgcagttc
     4801 acgattttca cgttccagct ctttcagacg ctgacgttca gcgctggtga gcccaccatc
     4861 accgcccccg gtatcccgct catgctggcg aacccagaca cgcagagtct ccggcgtaca
     4921 gccaatcttt ggggcaatgg aacaaattgc cgcccactgt gagtcatatt cgccctgact
     4981 ttccagaacc atacgaactg cgcgttgacg gacttcgggg gaaaaacgag tatttttagt
     5041 catcctgttt acctctttct cagggagttt agtctccagg atttccgggg cggttcatgc
     5101 tttcgtgcgg tatacctctg gataaaaaca tcgcatcaca ttacgccaag catggatact
     5161 gaataaatct aatcctggaa tgagcacaga caaatgccag gcgggatttc aggagattca
     5221 gtgaggtata aatgggaaaa atgaaagaac tgctgtttga aattcaggaa gaacgcactg
     5281 atgaatggat tgcggaaaat tacacggatg ctgaggaagg aactcctgaa tgggatgcgg
     5341 cagcacagga atacagctgg tttcaggact ggatggaaga ggcggctgag cagcaatatt
     5401 ttgaggcttc gcttgccagt attcctgacc gtctgcagga tgcaaaagat gaattgtttg
     5461 agttagagaa cctgatgcag ttcaaccaac cgggaattgt ggagcgaatg gcttacgttc
     5521 attgtgtgag tgtgttggac agttttctga tgtacagcgc aagggcactt cttaatcatc
     5581 ctccacattt gcagcgattt ctgcaggtag ctgattcgct gattgctaat aaggaagaca
     5641 gaagaaaatt acgtgcttcc aaatgggtcg agcaggaacc ggatacagcc actcctgaga
     5701 aggtttatac ctggcgagct cagtctcttg tggcgaagaa aacgtttcag agccacaaag
     5761 tgatagatcg gtatttttca acgatgctgg caacaccgca tgagtggcct ttgtaggaac
     5821 ttgagagggt aatcaaggtc cgaaacgcac tggtacaccg tgatggagtt accgaatctc
     5881 ttgaacccgt gtatatcagt tcggggagtg tgcagaatgc tatctgcacg gtacgagcat
     5941 ttattaccgc ggcggctgaa acccttctgc aggaagatgc gctatacagg acggatgatg
     6001 gcacgttctg aaattaccgc agcgacacca tccataagcc tcctcttcac tgagggatat
     6061 gtcgatggtt catgatgaac tgccaaaata cctgctggtc actctgaagc agcggacaga
     6121 aaaagcagcc aggacggcag gaagaacgcc agccgggcag tagactcacc ggctggttcc
     6181 gctctccgac tcttggtacg tcagccagct gcagacgatg gtggccacac tgaaaatccc
     6241 catggaacgg cgtaataaac ggacaggcag gagagagaaa gcgcggatct gggaagtgac
     6301 ggacagaacg gtcagggcct ggattgggga agcggttacc gctgacggtg tgacgttatc
     6361 tgtcccggtc acgccacata cgttccgcca ttcctatgcg atgcacatgc tgtatgtcgg
     6421 tataccgctg aaggtcctgc agagcctgat ggggcataag tccatcagtt caacggaggt
     6481 ctacacgaag gtatttgcac tggatgtggc tgcacggcac cgggtgcagt tttcgatgct
     6541 tgagtccgat gcggtgtcta tgctcaaaag aattccttaa gctctgactt ttttaaatcc
     6601 ctttcggata tctcatggat atattatcgg tatttcatat tctgttgaca tgccgggccg
     6661 gtatagagta aaaaaaacat ctattgagta cctcttggat atccgaaagg tgtctttggg
     6721 gggctttatg ggtattacat gtggattctg gagatacact atgcctgtta ttgctaatac
     6781 gcatcccaaa ggtggtgtgg gtaagacaac ttcatccgta aacattgttg gtgaaatgaa
     6841 gtctgataca gttgatctgg atactcatac cgggctttct atcattctgg ggctgagacc
     6901 tgaaggaaaa gaaatttccg tgaaagtacc caaaacagtg gatgagttaa tcgaaattat
     6961 gactccctac aagaacagcg ataagacgtt acttattgac tgtgggggat ttgactcaga
     7021 tcttacccgt actgcgattg cctttgcaga ctgcgttatt gtcccttcaa aagactccct
     7081 gactgagcgt attggtttga tgcattttga tggagtactg gacgaaatca gctctatcat
     7141 ggggactgat ataactgctc acctgtatct ctgtaaagtt aatcctaaca agaagaaatt
     7201 ccctaagctt gatgcaatcc tgccatcgtt caaacatctg aagctgatga agagccgcat
     7261 ttctgctcgt gcagaatttg aggatgtcat tgagactggg atgggaatca ctgagtctgt
     7321 tcatgggcgc tattcggcag gaggtaagga agttattgcc ctgatcgaag aaatcaatca
     7381 tctgattgaa aataacaaac agtaaagtgt atctattggg tatccatata gtattctttg
     7441 ggtttttaaa ggatatcttt tgagtatcta acaaacatcc tgggttatct catgagcaaa
     7501 gcgaaattta gtgcacaggc tgaaaaactg aaaaacctgg tggattctgc cagattacaa
     7561 acttcttctt ctgccgtaac agcaacagtc ccgtctcagc caaatgaagg tacagaggaa
     7621 gtttcaggtc gtcccgtggc cgccaggacg aaggctaaga atcttaaggc cataccgctg
     7681 tcgtattttg aagctcatgc ggaacttaaa gcgaccagta aaacatctct ggattttagc
     7741 tcatatatta ttgaggctat aagagagaag cttgaacgtg atggcgcaat aggtcaataa
     7801 gctatcggat accttaaggg tatctaatgt gtgtctttta aatgcgttat ggggattctt
     7861 agtgtaccta aagagtcccc attagacatc tttctgtata tggtgacatg attcgtcacc
     7921 gctttccttt cctctcagtt tcaccatttc attcccttcc cttaacctga accatgatgt
     7981 caaagatatt tttatattgc atttatgtat cacatataac actcataatg ttttttgtat
     8041 ctatgatttt ctctggtctt aaggttgtac gtgcgatata catgcagttt attgtgtttt
     8101 tatgttgctt gtaccctttc agaaagacca tctttccatt ataattgaat atacattgca
     8161 tgtatgaagc atatttgttt tttctatttg tttatgagca acggagactg gttatgaaaa
     8221 tctttattga tgatggttct accaacatta aacttgcctg gttggaggat ggtgacgtga
     8281 aaacgttaat cagccctaac agctttaaac cggaatggtc ttttagttta ctggatgatg
     8341 ctgccccggc taattacgag atcgacggcg agaagttttc atttgatcca ttaagtgctg
     8401 atgctgtcgt aacgactgaa acacgctatc agtacagtga tgtgaatgtt gtggccattc
     8461 agcatgcgct acagcagacc ggcctgaaag cgcagcctat tgatgtcatc gtcacattgc
     8521 cgatctctga ataccttgac gccaacaatc agaaaaataa gcaaaacatt gaaagaaaga
     8581 aaaaaaatgt gatgcgtgaa gtacgggtgc aggggagtga tgcatttgtt attcgcagtg
     8641 tttccgttct tcctgaaagt attcccgctg gtttcagtgt tcttgcgggg cttgaggatg
     8701 atgaatccct tttgatcgta gatctcggtg gtacaactct tgatgtgtcg catgttcgta
     8761 gcaaaatgac agggattaca aaaacctggt gtgatccgaa tatcggcgtg tccctgatta
     8821 catccggggt taaggaacag atggctgtac atgctaatac aagagtgagc tcattccagg
     8881 ctgacaatat cattgtgcac cgtaatgaac ctgactacct ttcccgtcgc atttataatg
     8941 ctgaacagcg tgagtctatc attaatgtta tcaatgaacg gcagaagttg cttattaaac
     9001 gagttaatga tgtcatcagc cgtttcaccg actatacaca tgtaatgtgt gttggtggtg
     9061 gggctgaaat tgtggccgag gctgtgaaga acctgactaa ggttcctgat gagcgctttt
     9121 atctgagctc atccccgcag tttgatttag tcatgggaat gataaaaatg aaaggtggtg
     9181 tgactaatga gtgatgaaaa taaatccaga cgctgctcat ttgaactttt cccggatgaa
     9241 cggacaggag ataagatagc agatgagctc attgcaaatg aaaaactgaa ggagcgggga
     9301 cgtttcatgc gcgcgatgtt agttactggt gcggcgtttg cagctataga taaacgtctt
     9361 cctttgctga tatcggagtt actgacagaa aatacgactc tggatgatat taataaggtg
     9421 atttccagcg taataccagg tgctttttcg gttgaaaaga aattgcttga attactggaa
     9481 aaacaatctg gtctccatac gtctgtggat tgcagtactc ctcttacaga gcagagtctg
     9541 tcgagaaatg acggtgaaga ccagaccaga agaaacgctg aaaatatgtt cggagatgac
     9601 tgaactaacc cagcctggcc aggggaatat ctcgccattt agttgtataa cactgactca
     9661 acatttcccg tttcatttgc cattcagggg cggttcctcg ccccgcaaac cacactttcc
     9721 cttttcccga ctggttcagt tcatcgagtg ttttcattaa cttttcgctg tttttatggg
     9781 gctggatttc atcaaataat cccggctgcg ctatacccga tggtgtgaaa tcagccagca
     9841 tgactcctgc cttcatatag cggtaccctt cacgccagac atggtttaac gctctgcatg
     9901 ctgcggcaat aatgtcccgg ctgtcctgtg taggcaatgg aagcttttcc acagcggaat
     9961 tgctgtaaca ggggctttca ctgcaaaggg tgacgttcgt ataaatgtcg ccacctgccg
    10021 gcaatactga cgctccccac gtagtttctc tgcggcccgc tctgcatact gaacaacagc
    10081 ctggtgcatg gcatctttgt ctgtgattcg ttcaccaaaa ctgcggctac agacaatctg
    10141 ctgttttgcc ggtggggctt cttccggaga tatgcaggac tcgccgttga gttcgcgtac
    10201 tgtatgctca agaatgacgc tgaagttttt ccggatgaat gccgtgttag cctgcgccag
    10261 ctgctgcgct gtgttaatac cctgcgcatt cagcttttcc gtcagtctgc gtcctactcc
    10321 ccagacttca ccaacaggct gctgccccag taacttgaag gtccgattac ggttttctgc
    10381 cgtcagcgcg accacaccgg aaaactgtgg ccattgcttt gttgcccact gtgcactttt
    10441 ggccagcgtt tttgtaggtg caatgcccac ccccatggcg agtcctgtcc agctctttac
    10501 ttgttccctg agctgatgac caaaaacctc cggagagatg caacggttca ccccccgcaa
    10561 atcaatgaac aacgtgagtg acaacaccaa aaatacagat gttttccgga tacagtgtgc
    10621 ggaactccgg gctgtctgaa accggctcca gtgccgggcg tgggcgcaac agcagtcgtt
    10681 tgacggtgaa ctccccgtcg atctcagcga taacgatgtc cccgtgttgt ggtttttcgg
    10741 ccctgtccac taccagcaga tcaccattct gcacgccagc ctggttcatc gattcaccgc
    10801 tggcgcgcag aaagaaggtg gctgcaggtc tgctgatgca atagctgttc agatccagtt
    10861 cctgctcagc ataatcagtg gcaggcgaag gaaaaccggc ctggcaacga tcggcaaaca
    10921 acggtcgcac gtaactgtca ttgcctgaag ggtcagccgg acggtgataa acggtactca
    10981 tgctgcaggc agccagctgt cgtcctgcca gatttcttcc agcagagaca ggatacgttc
    11041 tttatccttg tcgttgcggg ctcctgtgac agatacagac ggggcgctgc cctggtctat
    11101 acgcagccag atttccgggt agtgtggttt cagacgtcgt tcgatttcat tctgtagcgc
    11161 ctgaagtgtg cctgatttga ggttttttgt gctctggcgg tcgaaaagaa tttcaatgcg
    11221 gatcatgttc tcatctccct tttcttttgc tgtatgcata tacagtattc taataactga
    11281 atccacacac agtcaaatgt cattattgtg actaaaaaac gctcaattct ctgagggtgt
    11341 ggctgtatgc ctgctgtgga cggctgtgtc cagcttatcc acagcatttt gtgcacggtt
    11401 ctgtggacaa aatacctggt tacccaggcc gtgccggcac gttaaccggg ctgcatccga
    11461 tgcaagtgtg tcgctgtcgc cggcctcctc acccggtcac gtttcgtcgt tcctcctcca
    11521 cgcgctgcgg cttcggggcc gcacctgcat tcgtatgcgg tcgcccggtt acaggtgcgg
    11581 cacggcctgg tggaggccgc atgtgagagg agaattccca tgccaaactg gtgctcaaat
    11641 gtgcgacatg aagtcgcctg aataattgtt ccagcggagt tcgattccgt cagggaacct
    11701 gatgttccgt catcagtagc ctactgacac atgcgtcact ggtaacggtg gggtgtgaag
    11761 ctgtcaggac aatgaaaccg gatcttcgga tcgcatgaaa ccgtgaggtt acatgtaatc
    11821 tgccagcatc aggcggagga ggtctaggct cggtagcatg actaacatat gtgaactgct
    11881 gaagcgtcgt taaccagaac aagccaaaga tgctgacagg cttgaaccaa aaggtatgtg
    11941 gttggttact cctctgtttg ctaggggtac acggacaata aaccaccggt gttgtgagca
    12001 gagtctaacc tactgttgtt attcaggtgg aacatggtaa gcccgtatcg ctgcctcgtg
    12061 aggcaggtta accgcaaggg atactgttgg cggtgcgggt aaaagaaggt ggaaaaagcg
    12121 aatgccagtc tgtaatggac cggataaggg ttgagcccgg caacattacc ctacgcgaaa
    12181 gcgggcagac ttccactggg tctttcatta cgagagagtt tgagtaatct tccagaaagg
    12241 aaaagcagat gactgagcag gctacaacct gtaaaggtgc gtccttactt aacggtgact
    12301 cctggcacag tatcaactgg cgtcagtgct atcgggaagt gcggagactg caagcgcgta
    12361 tcgtaaaggc aacacgagaa ggtaaacatg gcaaggtcaa atccttgcaa tggatactga
    12421 ctcactcgtt tagtggcaga gcagttgctg tcagacgagt tacagagaat agcgggaaac
    12481 gtactcctgg ggttgatggt cagacatggt cttccccgga ggttaaattt cttgctatta
    12541 acctgctgaa acggcgtggt tacaaaccac agccacttaa gcgggtttat atacctaagt
    12601 caaatgggaa gtctcgtccc cttgggatac ccactatgaa ggatcgggcg atgcaggcgt
    12661 tgtatttgct ggctcttgag cccgttgctg aagtcactgc tgaccagaga tccttcggct
    12721 ttcggactgg aaggtctact gcagatgcga tagcacagtg cttctgtgtt cttgcgcaga
    12781 aaacatccgc tgaatgggtg cttgaggggg atatacgggg atgctttgac aatatatctc
    12841 atcaatggct aatcgataac acctcaacag acagacaaat cctgacaaag tggcttaaag
    12901 cgggttacag ggaaaagggg cagttattcc ctgtaaattc aggaactcct caggggggaa
    12961 taatttctcc tgtactggct aacattgctc tggatggact ggaggcgctt cttgcttcgg
    13021 agtttaaaaa gagaacagtc aagggacgat tggttaaccc gaaagttaac tatgtgagat
    13081 atgctgatga ttttattatc actggagagt caaaagaact tcttgagtct caggtattac
    13141 ccgtcgtcag gcggtttatg gctgaaagag gattaatgct ttctccggaa aagacgaaaa
    13201 tcacacatat cgaggaagga tttgattttc ttggacagaa cattcgcaaa tatggcggta
    13261 agatgctgat caaaccctct aaagccaatg tcagttcctt tttaaagaaa atacgcgctg
    13321 taatcaaagg caacaaagct atggatcagc ttacgttgat ccgcatgctg aacccaatga
    13381 tcaaagggtg ggcggcttac catcaacaca ttgtggccaa ggtcgcattt aacaaagttg
    13441 acaatgagat ctggctggct ttatggcgtt gggctgttcg gcgtcatccc aataagggaa
    13501 agaagtggat tcggaaaaga tatttccatc aacaaggtgc ccgaaactgg tctttctcca
    13561 cggctaccgg agaactcctt gccaacggta agcctcagta tgcaaatctg aggaaagcaa
    13621 tcgatacgcc tatcaaccgg tttaaaccga ttaaaattgc agctaatccg ttcgatcctc
    13681 aatgggagat gtactttgag gagcgttgtg cagacaaaat gcggcacaaa ctgcaaggac
    13741 ggaaaaagct cattcaaatc tggtttgagc agcggggccg atgccccata tgtgatgaga
    13801 ggattacatc tgattcccag tggcaggttc atcacatcat acggcgagtc gatggaggga
    13861 gcaactgcct ctccaatttg attatgctgc atccgatgtg tcacacactt gttcatgcga
    13921 aaggtataca tgtagtgaaa ccggctcatg agagtgggct tagaaaggct tgagccgtat
    13981 gccgggaaac tggcacgtac ggttcttagg gggcggtgat gcagtaatgc atcactgcta
    14041 cccgatcgta tgcatttttc tggtgaaccg gcacagattg ctgagattaa acgactggcc
    14101 agcggtgcag tcacaccgtt ttatcgccgc gccacaaatg aaggtattca gctgtttctg
    14161 gccggaagtg ccggacttct gcagatcact gaaaatatcc gctctgagca gtgccccgga
    14221 gtgacggctg ccggacgtgg tgctgtatcg acggaaaata tcgcgttcac ccgctggctg
    14281 acacacctgc agaacggtgt actgctggat gagcaaaact gcctgatgct gcatgaactc
    14341 tggctgcaga gcggtactgg acagcgtcgc tgggaagggt taccggatga tgtcagggaa
    14401 accatcactg tgcatttcac cgcaaaaaga ggtgactggt gtgacatctg gggtaacgag
    14461 gatgtatcgg tgtggtggaa ccgtctgtgt gacaacgtag tgccggaaaa aaccatgccg
    14521 tttgacctgc tgacggttct gccgacccgc ctggatgttg aagtgaatgg ctttaacggt
    14581 ggtgttctga acggtgttcc ttctgcatat cactggtata cagaacggta tggcgtgaag
    14641 tggccttgtg ggtatgacct gaatatcagc agtcaggggg aaaacttcat tcaggtggat
    14701 ttcgacacgc cgtggtgtca gccggaaagc gacgttgttg cagaattaag ccgccgtttc
    14761 ggctgcacgc tggagcactg gtacgccgaa cagggctgca atttctgtgg ctggcagcga
    14821 tatgagcgtg gagagcttgt tgatgtgctg tggggggaac tggaatggtc ttccccgaca
    14881 gatgacgatg agctgccgga agtcaccgga cctgcgtgga tagtcgacaa ggtggcgcat
    14941 tacggtggat gaagtatgac ggagacgggc gggcaactgc ccgtttcttt tccggtaaag
    15001 gatgtcgcca tgctcctctt tttactgcgc cggctgacgc ggcgcggcat aaacgctgcc
    15061 tgtggtcagt gtcccgcgtc cggcgggcac gggacgggcg cttttaccgc tcccggctgg
    15121 cgtcggtgac agtgtacgcc agcctgtcgc ccttttctga tgaacgccct tcaagccgct
    15181 ttcgcggcat aaccttgccg tcaaacagac gacggctgcg gtattccacg gtcggcctga
    15241 cccgttaccg gacgcggtga acagcccaca ggcagcgggg aacgggcacc acaggggtgc
    15301 cctcccggtg ccctctgtaa gagaaggcgt tttgtatgtc ccgttttgtc ctcggtaact
    15361 gcatcgatgt tatagcccgt atccctgata acgccattga tttcatcctc accgacccgc
    15421 cgtacctcgt cggtttccgt gaccgttccg ggcgcaccat cgctggcgat aaaaccgatg
    15481 aatggctgca accggcctgt aatgaaatgt accgcgtact caaaaaagat gcgctgatgg
    15541 tcagcttcta tggctggaac cgcgtcgatc gctttgtggc cgcctggaaa aatgcgggat
    15601 tcagcgtggt tggtcacctg gtcttcacca aaaactacac atcgaaggcc gcatatgtgg
    15661 gctatcgcca cgaatgcgcc tacatcctgg caaaaggccg tccacgtctg ccacaaaacc
    15721 cgctgccgga cgtgctgggc tggaaatatt cgggcaatcg ccatcacccg acggaaaagc
    15781 ccgttaccag cctgcaaccg ctgattgaga gcttcacaca cccgaacgca attgtgctgg
    15841 acccgtttgc aggcagcggc tcaacctgcg tcgccgccct ccagtccgga cgccggtata
    15901 tcggtatcga gctgcttgag cagtatcacc gtgccgggca gcaacgcctt tccgccgtgc
    15961 aacgggccat gcagcagggg gccgcgaatg atgactggtt tatgccggag gctgcgtaaa
    16021 tgcactatgc cggacacgaa aaacttcgcg ccgaagtggc ggaggtggcc aatgccatgt
    16081 gcgacctgcg tacaaccatg aatgagatgg agcggcggta cagctttaat gctgacaccc
    16141 ttccggaacg tctggtgcgt cagacgttgt ttcgcgcaaa ccgcctcctg atggaggcat
    16201 ataccgaaat tctggaactg gatgcgtgct ttaaagattg agaaggagac gagaatgtac
    16261 ggaacatgcg aaacgctatg ccgggagctg gcagcaaagt atccgggaga cacgccgttg
    16321 atgctggttg tctggtcccc ggaagagatt caggccctcg ctgacggaat ggatatttcc
    16381 ctgtccgatc atgaaatcag gaccgtcctg gcgcgcctgg aggacatccc ggaagaccag
    16441 cgaattgagt ccggtatttc ttccgccgcg gcgatggaga ttatcagcaa cgtgagcgaa
    16501 aaccgccagg tgaccgtccc tgctgaactg ctggcgtccc tgattcagac cgctgaacag
    16561 gcattgtgga agcgtgaatg ggccgcccgt gattacggcc tcgccgtccc ggaatgcgtt
    16621 acccgccgtc aggcggtggt taatcaggcc cgcatcctgc tgaaaaacaa cacgcacgaa
    16681 aacgactgat gttatcgccg cctccgggcg gcgattcagg gagaccatga atcatgaata
    16741 aaacgctgaa tgcactgatt tgtcgtcatg cccgcgacct gctgctggcg cagggctggc
    16801 cggaagagac ggatgttgac cagcgtaacc tgaagtatcc gggctggatc agcatttacg
    16861 tgcggctgga tgcgccccgg ctggcgacgt tactcgttaa ccgccacgat ggcgtactgc
    16921 cgccgcacct ggcctccgcc attcagaaac tgaccggaac cggggcggaa cttgttctgt
    16981 ccggcagtca gtggcagtcg ctgccggtac ttccggcaga cggaacacag gtgtcttttc
    17041 cgtatgccgg tgaatggctg accgaagacg aaatcagggc agttcttgat gcggtgcgca
    17101 atgcagtacg aagcatctgt taccaggtgg cagacgatgc gcggcgtatc cgggcggcgc
    17161 tgaccaccac cggtcagacg ttgctgaccc gccagacgcg ccgctttcgt ctggttgtga
    17221 aggaaagcga ttacccctgc tggcttgatg aagatgacga aaatctgccc gtggtgctcg
    17281 atgccattct gaaccgtggc gcacgttttt cctctgtgga aatgtacctg gtctgtgagt
    17341 gtgtggagca tatcctggcc agtggtctgg tctgtgatgt gctgcgtata ccggatgaac
    17401 cgtcccgtcg ctggtttgac cgggatattc tgcgggaggt tgttctggag gcacgggatg
    17461 aaatccgcag catggcggat gccctggcaa aaatcagggg ctgatgatgg cggggaaatc
    17521 cccgcctttt tcctgccggt ccccggacag agcacaaagg aagtcgccgg cggctccgct
    17581 ttacccagcc atgcggggca tggccttgtg gattttcagt tatgtggccg ccgtgtcgtg
    17641 tgcgggctgt gccgtgcctc catcttagcc gggctggcag ggatgcaagg gtacgcttcg
    17701 ccgctgcggt cacccggtcc ctccttcccg tctgccgtga ttttccggtc gttcacccgc
    17761 tcagatttcg ctgtctcacc ctccaactcc cgtcagccgt gtgcgtcagg ctgcggcttc
    17821 ccttgcgccc tgtgcatccc cgccttatcg cccggctttt atggaggcac ggcaccgccc
    17881 ggtgtcgcgg aatatgtgaa caacggagga aaacatcatg cagtatgcga aacctgtcac
    17941 tctgaacgtt gaagagtgcg accgtctgtc ttttctgcct tacctgtttg gcctggattt
    18001 tctgtatgcc gaagcgtctg tatacgcgct ggcgaaaaaa atgatgccgg aatatgaagg
    18061 cggattctgg cacttcatcc gcctgccgga cggtggcggt tacatgatgc cggatggcga
    18121 ccgttttcat ctggtcaacg gtgaaaactg gtttgaccgc acggtcagcg ccgatgccgc
    18181 aggcatcatt ctgacttccc ttgtgattaa ccgccagttg tggctgtacc acgacagtgg
    18241 ggatgcagga ctgacccagc tttaccggat gcgcgatgcg cagttgtgga gccacatcga
    18301 atttcacccc gaatgtaacg cgatttacgc ggcactggac tgattaacgg acggggcggc
    18361 aatgccgccc ctgtaaagaa gacgagaaca tgtactgtta aagaaattat ccgcgatgta
    18421 ctggatacag acgtgccgga cagtgaatgc gttttggccg tggtgctgac ccgtggggat
    18481 gtgcgccata tggctcagga ctggaacctg tcagacgatg agctggaaac cgtcatgcag
    18541 cggctggacg atgcctttga atatggtgcg gatgtcagca ttgttcatga cgttgttcgt
    18601 gaactgatgg aagaaaagcg cgccagccgt caggtgacag ttccggcggt gatgctggaa
    18661 aaagtgatgg cgctggctgg cagtgaaatg aagcgcctgt atgccgtcgg gagtgagaac
    18721 gggggcgacg gtgacgcatt cgtcagggaa gaacgcgaag caatggacgt tgtgttacag
    18781 gggcaggcgg tggaggccgt ctgtcagccc gcgcgacccc ccctggcagg ctgacgcctg
    18841 ccggaaggga agcgggccac gctgacgcgt cgcccgttac ccttcccgtt tgtgatgtgt
    18901 ccggaatgtt tttttctgat gttatttttc tgtggttacc gtgcttgtta ttgttcttct
    18961 tcctcttctt cttttcgttg ctctcctgtt tatggctttc ccgggcgtcc cgcttgcggg
    19021 gcgggctgtc gtgaacggag ccagcggagc tgtctccgcc ctggcgggct tccatccctg
    19081 acgccgcaaa accccgggct tgcagcgacc ggaacggtcg cgccaccctt gtcgcgccgt
    19141 ggcctgtgct cctggcatcc cggcaaaagg ccgccgggat gtgaggcgca acgccgtttc
    19201 gcgcacgcgc tctccgtcat tgcggcgggt ccctgctcat tgcctgccgg atgccacaca
    19261 cccgtatgag tccctgtcgc cgggggctca tacagccaca acctgccgca ctgactgtcc
    19321 agggtaaatg aacagcccac aaaatttttt acccaaaacc cggtaaaaaa ttttgcgttc
    19381 tgcccaggac aggtgcgtca ggccgtggca gtgatgcccc ttgcggcgaa ggttctcaaa
    19441 cgggtgaagt tcataccaca gggaatacag cagcaggttc ccgcgaaacg tcaaaaccgc
    19501 ggctcccgaa actcaacggg tgatgttctt taaaaatcag gatttatttc aggctccggc
    19561 aatacgcatt tatgtgttct gtcagtcatc cggctggcag gtaatgagaa cgcaggaaca
    19621 gaaggaggaa aacaccggag aacgtcagac gcagatatga aaaaagcagg tcagggacct
    19681 gcttaatcca caccgcagag actgcacgaa cagtctttac ggtttcatta attaacgtca
    19741 taagaggcta aattatgaca gtaagtattg tttcaccttc cgcagcggct gtaaagcccc
    19801 gccgtcatcc ccgtttcaga cgcgaagaca tccccgcgcc ggaaattgac cccgtgctga
    19861 aagccttcgg gcgacacatt gcccggagtt ttcaccgggg gcgtggtgta cacatccccg
    19921 ccatgaaaaa cacggccttc gggcaggtgc tccgcacact cgaactgaaa cgcgctttta
    19981 actgatgagg tgagccgccc cgcgaggggc ggcaacagac ggaaggagaa cattttatga
    20041 catcagcagc aatcaccatg accgccccgg aagccgccag ccctgtgcag atgtaccgcg
    20101 cgacctactc accggatgac aacaaactgc gcctgtatgc cgtgtcacgt cttgacccgg
    20161 agacgtataa aaaagtgcat gatgccggtt tccgctgggc accaaaacag gcgctgtttg
    20221 tcgcgccagc ctggacaccg ggccgggaag acgtgctcct ctcacttgcc ggagagattg
    20281 aggatgaaga cagcacgctc gctgaacgtc aggaagcacg ggcggagcgg tttaccggat
    20341 acagcggaaa gcgggccagt gaatccgcac aggcacttga tgaagtggaa agactggccg
    20401 cgatgatccc gcccggtcag cccattcttg tggggcatca cagcgaacgc cgcgcccgtc
    20461 gtgatgcgca gcgtattgaa aacggcatga aacgagccgt gatgctcttt gaacgtgcgg
    20521 aatactggga agagcgggcg cggtcggcac tgcttcacgc gaagtataaa gaacgtccgg
    20581 acgttcgctg gcgtcgtatc aaaaaaatcg aagctgattt gcgcaaggct gaaaagacca
    20641 tcgcgcagtc gcagaaatat ctgacgatgt ggcgggctga atcgctggat ctgaatatgg
    20701 caaaactcat cagcagtcat gaccatatca gcgcctgttt cccgctggat acgtatccgc
    20761 gcccggcaga aaaaagccag tatgaaggaa gtcgatcgtt atggtcggcc ctggatgatg
    20821 acatcatcac cacggagcag gcccgcgaaa ttgcgatccg ctgtcatgaa cggcagattc
    20881 agcatcaaca acgctgggtt aaccactatc agaaccgcct gatctatgag cgtgccatgc
    20941 tggacgaaag cggcggcgtg gttacccgga cacaggattt tgagccgggc ggacaggttt
    21001 tcagccgggg cgagtggctg accatcatcc gtgtgaacaa aagcaacggg gcggtgagtt
    21061 cagtcacaac gccaaattac agttttctcg ggtacagcgg cacgatgaaa gtgacgcccg
    21121 atcgcatcac ggactacaaa gcaccatcgg cagaagaggc tgccgtcgcc agtcaggccg
    21181 cgaagcgtcc gccggtagtc aactatccgg gggaaggttt ccgggaaatg acaaaggcac
    21241 agtgggccgc cctgccccgg gactgtaagg ccgtgcgcag tgtggcagaa gcagaagacc
    21301 acggggcata ccggtaccgc cgcacaatgg acaataattt ccgcctggtg aatgtgtata
    21361 tcaccgacat gaaaatcacg gaaatcccac agaaataagg tatatccccc cgggaaaatc
    21421 cccgggggat gacggagata atgatatgca cagtcagtta agagaacgta tccggctgat
    21481 gcgcgcaagg ctggataacg ccgcgccggt tgctgaaatc cgggctgaat ctcagctttt
    21541 tgtgactcct gccccggtct gtgatcgcct ggtgatgctg gcggagatca gcaaccgtga
    21601 tcacattctg gaaccctctg ccggcaccgg ggccatactg cgggcgattc gggataccgc
    21661 gccgggggcc atgtgtgatg cggtggaaat caacagcggg ctggtccggt atctgcggga
    21721 aaattttaac ggtgtcaggg ttcagtgtgg cgacttcatg gaatggcagc cagtgcagta
    21781 ttacagccgg gttatcatga atccgccgtt cagccacggg caggatatcc ggcatatcct
    21841 gcgggccttt tccctgttgc gtccgggtgg cgtgctggtc gccgtctgtc tcaacggacc
    21901 acgccagcag gagaagctgt taccgttttc tgacgtccgc gaggagctgc cgcgcggcac
    21961 gtttgcttat accgatgtcc cgacgatgat tattcgtctg cgtgcctgat gacacagggc
    22021 cggcgggagg ataaatgcct gtctgcccgc gccggtggcg ctgaaagggg ccgcgctcac
    22081 gcgccgcccc tttcattacg cggacgttct gatgggggct tctgtttctt catgcctgtc
    22141 atttgtctgt tactgatgat gttgttgttc ctgctactgc tactgctact gctactgcta
    22201 ctgctactgc tactgctact gctactgttc ttctttttcc tgctgctctg attctgacta
    22261 ctgcagtgtt gtcctgtctg ccgggcgcac cttc
//
LOCUS       DAZFQD010000079        22273 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.079, whole genome shotgun sequence.
ACCESSION   DAZFQD010000079 DAZFQD010000000
VERSION     DAZFQD010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22273
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.079"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(1..339)
                     /locus_tag="ACHKTW_003056"
                     /pseudo
     CDS             complement(1..339)
                     /locus_tag="ACHKTW_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579104.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(568..1488)
                     /gene="malM"
                     /locus_tag="ACHKTW_003057"
     CDS             complement(568..1488)
                     /gene="malM"
                     /locus_tag="ACHKTW_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HFO6464032.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLNVPGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQEPGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTTDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPAPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
     gene            complement(1731..3071)
                     /gene="lamB"
                     /locus_tag="ACHKTW_003058"
     CDS             complement(1731..3071)
                     /gene="lamB"
                     /locus_tag="ACHKTW_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HFO6464033.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYNGDSKVNPNYGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            complement(3143..4258)
                     /gene="malK"
                     /locus_tag="ACHKTW_003059"
     CDS             complement(3143..4258)
                     /gene="malK"
                     /locus_tag="ACHKTW_003059"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HFO6464034.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIHIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            4623..5813
                     /gene="malE"
                     /locus_tag="ACHKTW_003060"
     CDS             4623..5813
                     /gene="malE"
                     /locus_tag="ACHKTW_003060"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HFO6464035.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            5967..7511
                     /gene="malF"
                     /locus_tag="ACHKTW_003061"
     CDS             5967..7511
                     /gene="malF"
                     /locus_tag="ACHKTW_003061"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HFO6464036.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ERAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGETGKNYLSDAFKFGGEQKLQLK
                     ETTAQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGNGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLIFLLVGALAIVNLKA
                     TRMKFD"
     gene            7526..8416
                     /gene="malG"
                     /locus_tag="ACHKTW_003062"
     CDS             7526..8416
                     /gene="malG"
                     /locus_tag="ACHKTW_003062"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HFO6464037.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            8788..10263
                     /gene="xylE"
                     /locus_tag="ACHKTW_003063"
     CDS             8788..10263
                     /gene="xylE"
                     /locus_tag="ACHKTW_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
                     /protein_id="HFO6464038.1"
                     /translation="MNTQYNSSYIFSITLVATLGGLLFGYDTAVISGTVESLNTVFVA
                     PQNLSESAANSLLGFCVASALIGCIIGGALGGYCSNRFGRRDSLKIAAVLFFISGVGS
                     AWPELGFTSINPDNTVPIYLAGYVPEFVIYRIIGGIGVGLASMLSPMYIAELAPAHIR
                     GKLVSFNQFAIIFGQLLVYCVNYFIARSGDASWLNTDGWRYMFASECIPALLFLMLLY
                     TVPESPRWLMSRGKQEQAESILRKIMGNTLATQAVQEIKHSLDHGRKTGGRLLMFGVG
                     VIVIGVMLSIFQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVINLTFTVLAIM
                     TVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQAPGIVALLSMLFYVAAFAMSWGPVCW
                     VLLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVAHFHNGFSYWIYGC
                     MGVLAALFMWKFVPETKGKTLEELEALWEPETKKTQQTATL"
     gene            complement(10307..10717)
                     /gene="psiE"
                     /locus_tag="ACHKTW_003064"
     CDS             complement(10307..10717)
                     /gene="psiE"
                     /locus_tag="ACHKTW_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HFO6464039.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            10932..11210
                     /locus_tag="ACHKTW_003065"
     CDS             10932..11210
                     /locus_tag="ACHKTW_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6464040.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            complement(11257..13353)
                     /locus_tag="ACHKTW_003066"
     CDS             complement(11257..13353)
                     /locus_tag="ACHKTW_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HFO6464041.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYNDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRVTENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETTLAQKRVEPVVPQSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTTGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(13353..14090)
                     /locus_tag="ACHKTW_003067"
     CDS             complement(13353..14090)
                     /locus_tag="ACHKTW_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HFO6464042.1"
                     /translation="MIKQTIVALILSVGASSVFAAGTVKVFSNGSSEAKTLTGAEYLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAELAADSSADDAAAINALRQ
                     QIQALKVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPSTVTLFGLISRPGKQP
                     FTPGRDVASYLSGQNLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSETPDALNADILQTLTQRIPQ"
     gene            complement(14087..14725)
                     /locus_tag="ACHKTW_003068"
     CDS             complement(14087..14725)
                     /locus_tag="ACHKTW_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HFO6464043.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVIQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     PVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            complement(14839..15081)
                     /gene="yjbE"
                     /locus_tag="ACHKTW_003069"
     CDS             complement(14839..15081)
                     /gene="yjbE"
                     /locus_tag="ACHKTW_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide production protein YjbE"
                     /protein_id="HFO6464044.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAGSAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTTTTTTTSTQ"
     gene            complement(15436..17085)
                     /gene="pgi"
                     /locus_tag="ACHKTW_003070"
     CDS             complement(15436..17085)
                     /gene="pgi"
                     /locus_tag="ACHKTW_003070"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFO6464045.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FGDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHIAEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRD"
     regulatory      17322..17518
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            17610..18959
                     /gene="lysC"
                     /locus_tag="ACHKTW_003071"
     CDS             17610..18959
                     /gene="lysC"
                     /locus_tag="ACHKTW_003071"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HFO6464046.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNDGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVTLTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHFNLFE"
     gene            19104..20030
                     /locus_tag="ACHKTW_003072"
     CDS             19104..20030
                     /locus_tag="ACHKTW_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFO6464047.1"
                     /translation="MATLMEKDALLNGASQCIAFLSNIIDNCSVSSHQDSGDALKRLV
                     SYRDYLYSTPAELVDFTQGKILLQQVRTQYQHEFNNTTHSENKASFDSIWQRLTNHEV
                     TPQQHPIGFVLGGQPGAGKSSLIELAKRETKDNIMIINGDDFRFLHPDFNYIYQNYGD
                     DFVTHTAKFSGETVERAIERAIVSKLNIVVEGTFRNAATPLQTLKKLKDAGYQTEVMI
                     KTTSAALSWESTNERYSKDKEAGNIARKVDKNHHDIVTGLLAENARKVFASNLSDKFA
                     VYSREKMIFSSQAATNDDIATLIQNEISGNTQ"
     gene            complement(20066..20857)
                     /locus_tag="ACHKTW_003073"
     CDS             complement(20066..20857)
                     /locus_tag="ACHKTW_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HFO6464048.1"
                     /translation="MPLNGVSAAMRERVSQQLKEIERRYDVKVLYACESGSRGWGFAS
                     PDSDYDVRFLYVHPLEWYLRVESPRDVIELPIDDELDVSGWEWRKALGLLKGANPTLI
                     EWLDSPVIYQQDEETITAFKAMVPMWFSPLRARWHYYSMAQKNFPGYLQGDEVRLKKY
                     FYVLRPLLAVRWVEAGKGVPPMRFAELLAGSELDAPLRAEIDELLERKQRAGEAEYGP
                     RRPLLHAFIRAELARGEIPPLLPDSREGDVKELDSLMYQTVMRRA"
     gene            complement(20863..21990)
                     /locus_tag="ACHKTW_003074"
     CDS             complement(20863..21990)
                     /locus_tag="ACHKTW_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HFO6464049.1"
                     /translation="MIKKISVRKDQLALLSRNGDYYKVLHAGEHLLPWLNTPEVLLIT
                     LDGSEVPDVLADYLRRFQPDWVEKYCLVADLSEIEAGALYMDGILQEILPPSTRRLYW
                     RVEDDLTLVRMNTQQVQVQTEVMNAVLQPRRKGAVKGRDAILTVQVPAWHVGVLKIDG
                     ETQALLPPGLTAYWKINHLVDAEVVDTRLQVLEVSGQEILTKDKVNLRINLAANWRYS
                     DVLLAFSQLTKPIDHLYRELQFALREAVGTRTLDELLEDKQVIDDVVSEQVKSRMLPF
                     GMEIASLGVKDIVLPGDMKNILAQLVEAEKSAQANVIRRREETAATRSLLNTAKVMEN
                     NPVALRLKELETLERVAERIDNISVFGGLDQVLHGLVNIKG"
BASE COUNT         5332 a         5789 c         5542 g         5610 t
ORIGIN      
        1 acttgttaaa tccaaatcag acaggtcgat atacccctgc gcatctttat ccatcagaaa
       61 attgcaagcg cattcaattt ttttcatttc tgtagaattt tacacttata gtatcattac
      121 tgattgagac ttcacgaaac aaatttattg catgattttt tcgttccgtt gttttatatt
      181 tgaaaaaata ttgctgatat aaataaagtt atctctgttt aaacctggca ccccattgaa
      241 attacgtagt aaatcaagat ctactatatt atttccttgc accacatcat gtttcatcaa
      301 agagttattg ccgccagaat ttgggatatg acttatcata aaacctctgc ataacagaaa
      361 taaaataaca tggaaaagta gcctgaatgt ccagacacta ccggcgaaag gaaaagaatc
      421 tctgataagg cattgagata atggatattc ttagtgaaga agaagtgagt tactataaaa
      481 aatcgctcat atcccaggaa ggaagcataa aaaaaggtgc gccaggagac gcaccagttg
      541 ttgcaaaatc agcactgtgg ggcgtaatta ccccttgcct tttacactgc tgataaaggt
      601 ggaacgggca gatgtcgatc ccaggcgttc agcttcatca agcagtttta acgccttatc
      661 aacatcacct ttcgcgacag cgtttttgat cgcggtatta aaataacttt ccgtgtcgtt
      721 gagcatcggc tcgcttttct tcaccggtgc cggagcgggt gcagccacag ctggtgccgc
      781 cgtgttacct accgtaaccg gagctggagc ggaagaacca aataaaggtc ctaccaacac
      841 gctggagctg gagttcgttt tcactttcag tttcagtaag ccatcggtgg tatgacgagc
      901 aaccggatcg gggatatccg ggatcgagtt accgacgccc ttggcatagg ctttagccgg
      961 gtcgagcagt tgggtcgtct gctggagatc tttttccgtg gtaaagacca gaacataaag
     1021 tttttgctgc cccaacgccg gtgtcaggcg cataacgcct tccagccgat ctgcactcat
     1081 cacgcctggt tcctggtagg tgaaataact gctggggaag aaggctgatg gggtcatgtt
     1141 ctgatcaaga atcagcacgt tcggcgcaaa aacgctggtt tgtttgttca cttcgctggt
     1201 cagcgtcagg gtcagttcgc caatgtttgc cgggacgctg tacgcagcaa ccggaccact
     1261 gatgccggga acgttcagtt gttggccgcc ggtcgccagt tgggtggtct gggttttaga
     1321 ttgatcgacc ggtgtccagg tgagttgttg cagcgcggca gatggaatgg ctggcgcgtc
     1381 gctggtgttt tgcggtacgt agttaacatc ggcaaggctg attccaggcg cgcttgccag
     1441 taaccctgct gataaacaga gggcgatgag acttttattc attttcattg ttttcacctc
     1501 aaaatctgga gctcagcggt agccaggcaa tagcgcgcta aacccgataa tcagaggggc
     1561 tttcgcccct ttcagataat gacaacctgt ttttatgccg gatgcggcgt aaacgcctta
     1621 tccggcctac atttgacagc cgttgtaggc ctgataagac gcgcaagcgt cgcatcaggc
     1681 gttggttgcc gaatgcggcg taaacgcctt atccggccca ggttttgcta ttaccaccag
     1741 atttccatct gggcaccgaa ggtccactcg tcgctgtcgc cacgaccgaa gctgccgccg
     1801 ttgaaatcag caggaacggc tttgccgtag ttcgggttaa ccttgctatc gccgttgtag
     1861 tcataacccc atttctcatc ccacttggcg taggttgcga agacacgaat agccgggcgt
     1921 gaccagatgc tgtcgccagc ctgccattgt tgtgcgaggg taattttgta ctgattgttc
     1981 ttgtcgccgg tgcgctggga ttcgacgttg tcgtagccga tttccatcac ggtgctcatg
     2041 attggcgtcc acttgtacat cgggcgaata ccaacggtcc accacttggt gccgttgtcg
     2101 ttatcccagt tgatatcctg gtacataccc acgtacatca tgtcccagtt gtcgcccatg
     2161 gagatcgcac cgtggtcgag gatacgcagc atgtgaccgt tgttgttgat attgtaggca
     2221 aatttttcgt tatcaaacgc gacgccagaa ccctgcgaca gacctttacc ctgcgaggtc
     2281 atcgagtcag tagcgtactg aacaacaaac ttgttaaagc ccttcaggac actctgagta
     2341 tgttcagcag tgaataacca gccgtctttc gatgcgccat caaccagacg atagttatca
     2401 cgcaggttgg cacgaccgta gtcgacaccc agttctaatg tgccgcccgg gttgatttcc
     2461 atctgcgcta aacgcacatc gaaaacgtcg ttcgcggttt cgttggtata gtcataaata
     2521 ttgttgctgg caaaagagga agaaccacca gcttcagagg agcgggttgc tgccagagag
     2581 agtttaccga agccaacatc gatgttttcc agaccggcac caggaccaga aatatcccag
     2641 tagtagaagt cgatcatatg aacgtcatga cgttggtaga agcgcttacc tgcccagatg
     2701 gtggaacctg gcagccattc gatcaggttt ttaccctgca cgtttgcttc acggaaggcc
     2761 ggatcggtag cttcccagtc attctgttgc gcgacggaat aggccacgtt agtgtcgaaa
     2821 tagaagctct tatcgccctc tttccacact tcctgaccca attttaattc agcataagtt
     2881 tcacattcgt tgccaagacg gtatttactt tgagcaccgg tagtctggaa acactgttgt
     2941 tcaccgccgc tacctgtcca gccaataccg gaacgtgcat agccgtggaa atcaacagcc
     3001 attgcctgag cagacattac gcccgctgcg acggcaaccg ccagaggaag tttgcgcaga
     3061 gtaatcatca ttctatctcc tgagtcattg cttttctttt ttcacatcac ctgtgacagg
     3121 ctttgtgtgt tttgtggggt gcttaaacgc ccggctcctt atgcagtcga cgacatgcag
     3181 tgccatcctc acggaacaga tggcaacgct ctggcggcag gccgatagcg aatgtggcac
     3241 cttcttctac caacaccacg tcgttctggc ggtacaccag gttttgacga atggaaggga
     3301 tctggatatg gatttgggtt tcgttgccga gttgctcgac gacctgaact tcaccctcaa
     3361 ggatgacgtc agcgatatca ctcggcagta gatgttccgg gcgaataccc agcgacatat
     3421 tggctccaac ctggacatca cggctttcaa ctggcagcca gacttgctga cgatttggca
     3481 tcggcagctc cacctgcact tgatcgattg cggtggcggt cacttttacc ggcaggaagt
     3541 tcatctttgg cgaaccgata aatccggcga caaaacggtc tgccggatag tggtacagct
     3601 ccagcggttt cccaacctgc gccacgcgac cggcgtccag caccacgatt ttgtcggcca
     3661 gcgtcatcgc ttcgacctga tcgtgggtga cgtaaatcat tgtgcggccc aggcgtttat
     3721 gcagacggga gatttcgata cgcatttgca cacgcagtgc agcatcgagg ttggagagcg
     3781 gttcatcgag caaaaatacg cttggctcgg ccaccagcgt acggccaatc gccacacgct
     3841 gacgctgacc gccagagagc gctttcggtt tgcgatccag caaatgcgcc agttgcagca
     3901 cttccgccac ctggttaacg cgttggttaa tcacctcttt ttttgcgcct gccagtttca
     3961 ggccaaatga catattttct gctactgaca ggtggggata gagcgcgtaa gactgaaaca
     4021 ccataccaac gccgcgttct gctggcggag tgtcattcat ccgtttctca ccgatgaaca
     4081 ggtcgccgct ggtgatcgtc tcaagcccgg caatcatgcg cagtaaagtc gatttaccgc
     4141 agccagacgg tccgacaaac accacgaatt caccttcatg gatatcgaga ttgatatctt
     4201 tcgataccac gacctcgccc caggctttcg ttacattttg cagctgtacg ctcgccatgc
     4261 ccttctccct ttgtaacaac ctgtcatcga cagcaacatt catgatgggc tgactatgcg
     4321 tcatcaggag atggcttaaa tcctccaccc cctggctttt ttatggggga ggaggcggga
     4381 ggatgagaac acggcttctg tgaactaaac cgaggtcatg taaggaattt cgtgatgttg
     4441 cttgcaaaaa tcgtggcgat tttatgtgcg catctccaca ttaccgccaa ttctgtaaca
     4501 gagatcacac aaagcaacgg tggggcgtag gggcaaggag gatggaaaga ggttgccgta
     4561 taaagaaact agagtccgtt taggtgtttt cacgagcact tcaccaacaa ggaccataga
     4621 ttatgaaaat aaaaacaggt gcacgcatcc tcgcattatc cgcattaacg acgatgatgt
     4681 tttccgcctc ggctctcgcc aaaatcgaag aaggtaaact ggtaatctgg attaacggcg
     4741 ataaaggcta taacggtctc gctgaagtcg gtaagaaatt cgagaaagat accggaatta
     4801 aagtcaccgt tgagcatccg gataaactgg aagagaaatt ccctcaggtt gcggcaactg
     4861 gcgatggccc tgacattatc ttctgggcac acgaccgctt tggtggctac gctcaatctg
     4921 gcctgttggc tgaaatcacc ccggacaaag cgttccagga caagctgtat ccgtttacct
     4981 gggatgccgt acgttacaac ggcaagctga ttgcttaccc gatcgctgtt gaagcgttat
     5041 cgctgattta taacaaagat ctgctgccga acccaccaaa aacctgggaa gagatcccgg
     5101 cgctggataa agaactgaaa gcgaaaggta agagcgcgct gatgttcaac ctgcaagaac
     5161 cgtacttcac ctggccgctg attgctgctg acgggggtta tgcgttcaag tatgaaaacg
     5221 gcaagtacga tattaaagac gtgggcgtgg ataacgctgg cgcgaaagcg ggtctgacct
     5281 tcctggttga ccttattaaa aacaaacaca tgaatgcaga caccgattac tccatcgcag
     5341 aagctgcctt taataaaggc gaaacagcga tgaccatcaa cggcccgtgg gcatggtcca
     5401 acatcgacac cagcaaagtg aattatggtg taacggtact gccgaccttc aagggtcaac
     5461 catctaaacc gttcgttggc gtgctgagcg ccggtattaa cgccgccagt ccgaacaaag
     5521 agctggcgaa agagttcctc gaaaactatc tgctgactga cgaaggtctg gaagcggtca
     5581 ataaagacaa accgctgggt gcagtggcgc tgaagtctta cgaggaagag ttggcgaaag
     5641 atccacgtat tgccgccacc atggaaaacg cccagaaagg tgaaatcatg ccgaacatcc
     5701 cgcagatgtc cgctttctgg tatgccgtgc gtactgcggt gatcaacgcc gccagcggtc
     5761 gtcagactgt cgatgaagcc ctgaaagacg cgcagactcg tatcaccaag taatgctatg
     5821 aaatgccgga tgcggcgtga acgccttgtc cggcctacaa aactgaaacg catgtaggcc
     5881 tgataagacg catcagtgtc gcatcaggca gttgttgtcg gataaggcgt gaaagcctta
     5941 tccgtcctgg aatgaggaag aaccccatgg atgtcattaa aaagaaacat tggtggcaaa
     6001 gcgacgcgct gaaatggtca gtgctaggtc tgctcggcct gctggtgggt taccttgttg
     6061 ttttaatgta cgcacaaggg gaatacctgt tcgccattac cacgctgata ttgagttcag
     6121 cggggctgta tattttcgcc aatcgtaaag cctacgcctg gcgctatgtt tacccgggaa
     6181 tggctggaat gggattattc gtcctcttcc ctctggtctg caccatcgcc attgccttca
     6241 ccaactacag cagcactaac cagctgactt ttgaacgtgc gcaggaagtg ttgttagatc
     6301 gctcctggca agcaggcaaa acctataact ttggtcttta cccggcgggc gatgagtggc
     6361 aactggcgct cagcgacggc gaaaccggta aaaattacct ctccgacgcg tttaaatttg
     6421 gcggcgagca aaaactgcaa ctgaaagaaa cgaccgccca gcccgaaggc gaacgcgcga
     6481 atctgcgcgt gattacccag aaccgccagg cgctgagtga cattaccgcc attctgccgg
     6541 atggcaacaa agtgatgatg agctccctgc gccagttttc tggcacgcag ccgctctaca
     6601 cactcgacgg taacggcacg ttgacgaata atcagagtgg cgtgaaatat cgtccgaata
     6661 accaaattgg cttttaccag tccattaccg ccgacggcaa ctggggggat gaaaagctaa
     6721 gccccggtta caccgtgacc accggctgga aaaactttac ccgcgtcttt accgacgaag
     6781 gcattcagaa accgttcctc gccattttcg tctggaccgt ggtgttttca ctgatcaccg
     6841 tctttttaac ggtggcggtc ggcatggttc tggcgtgtct ggtgcagtgg gaagcgttgc
     6901 gtggcaaagc ggtatatcgc gtcctgctga ttctgcccta cgcggtgcca tcattcattt
     6961 caatcttgat tttcaaaggg ttgtttaacc agagcttcgg tgaaatcaac atgatgttga
     7021 gcgcgctgtt tggcgtgaag cccgcctggt tcagcgatcc gaccactgcc cgaacgatgc
     7081 tgattatcgt caatacctgg ctgggttatc cgtacatgat gatcctctgc atgggcttgc
     7141 tgaaagcgat tccggacgat ttgtatgaag cctcagcaat ggatggcgca ggtccgttcc
     7201 agaacttctt taagattacg ctgccgctgc tgattaaacc gctgacgccg ctgatgatcg
     7261 ccagcttcgc ctttaacttt aacaacttcg tgctgattca gctgttaacc aacggcggcc
     7321 cggatcgtct tggcacgacc acgccagccg gttataccga cctgcttgtt aactacacct
     7381 accgtatcgc ttttgaaggc ggcgggggtc aggacttcgg tctggctgca gcaattgcca
     7441 cgctgatctt cctgctggtg ggtgcgctgg cgatagtgaa cctgaaagcc acgcgaatga
     7501 agtttgatta agggagataa caaaaatggc aatggtccaa ccgaaatcgc aaaaagcacg
     7561 tttgtttatt actcacctgc tactgctact ttttatcgcg gcgattatgt tcccgctgct
     7621 gatggtcgtc gctatctcgc tgcgtcaggg aaacttcgcg accggtagcc tgatcccgga
     7681 gcaaatctcc tgggatcact ggaaactggc gttaggtttt agcgttgaac aggctgatgg
     7741 tcgcattacg ccaccgccat tcccggtact gctgtggctg tggaactcgg taaaggtcgc
     7801 cgggatttcc gcgattggca ttgtggcgct ctccaccacc tgcgcctacg ctttcgcccg
     7861 tatgcgcttt ccaggcaaag cgacgctgct gaaaggaatg ctgattttcc agatgttccc
     7921 ggcagtactt tcactggtcg cgttgtatgc gttgtttgat cgtctgggtg agtacattcc
     7981 attcattggc ctgaatactc acggcggcgt aattttcgcg tatctgggtg ggattgcgct
     8041 gcatgtctgg accatcaaag gctatttcga aaccatcgac agttcgctgg aagaagcggc
     8101 tgcgctggat ggtgcgacac cgtggcaggc cttccgcctt gtcctgttgc cgctgtcagt
     8161 accgattctg gcggtggtat tcatcctgtc gtttatcgct gccattactg aagttccggt
     8221 cgcgtcgctg ttactgcgtg acgtaaacag ctacaccctg gccgtgggga tgcagcaata
     8281 cctcaacccg caaaactacc tgtggggtga ctttgccgcc gctgccgtga tgtctgcatt
     8341 accgatcacc atcgtcttct tgctggctca acgctggctg gtcaacggcc tgacggcagg
     8401 tggtgtgaaa ggttaaagat gttgttctgc caatgttatg ccgctgcacc ctcaacttac
     8461 gttatcccaa cttgtgactg ttattcggcg ctccacggag cgcctttttt tctttcgtct
     8521 gcaatctgaa tcgttcgccg gttaatattc ccatcataga gcttattatt tttacgttat
     8581 ttgttttccc acttacgata attctctttc gtgctctgag tcacggcaat agtattgttt
     8641 tcatcaattt ttgataatta tcacaattaa gatcacagaa aagacattac gtaaacgcat
     8701 tgtaaaaaat gataattgcc ttaactgcct gacaattcca acatcaatgc actgataaaa
     8761 gatcagaatg gtctaaggca ggtctgaatg aatacccagt ataattccag ttatatattt
     8821 tcgattacct tagtcgctac attaggtggt ttattatttg gctacgacac cgccgttatt
     8881 tccggtactg ttgagtcact caataccgtc tttgttgctc cacaaaactt aagtgaatcc
     8941 gctgccaact ccctgttagg attttgcgta gccagcgctc tgattggttg catcatcggc
     9001 ggtgccctcg gtggttattg cagtaaccgc ttcggccgtc gtgattcact taagattgct
     9061 gctgtcctgt tttttatttc tggtgtaggt tctgcctggc cagaacttgg ttttacctct
     9121 ataaacccgg acaacacagt ccctatttat ctggcaggtt atgtcccgga atttgttatt
     9181 tatcgtatta ttggcggtat tggcgttggt ttagcctcaa tgctttcacc aatgtatatt
     9241 gcggaactgg ctccagctca tattcgcgga aaactggttt catttaacca gtttgcgatt
     9301 attttcgggc aacttttagt ttactgcgta aactatttta ttgcccgttc cggtgatgcc
     9361 agctggctga atactgacgg ctggcgttat atgtttgcct cggaatgtat ccctgcactg
     9421 ctgttcttaa tgctgctgta taccgtgcca gaaagtcctc gctggctgat gtcacgcggc
     9481 aagcaagaac aggcggaaag tatcctgcgc aaaattatgg gcaacacgct tgcaactcag
     9541 gcagtacagg aaattaaaca ctccctggat catggccgca aaaccggtgg tcgtctgctg
     9601 atgtttggcg tgggcgtgat tgtaatcggc gttatgctct ccatcttcca gcaatttgtc
     9661 ggcatcaatg tggtgttgta ctacgcgccg gaagtgttca aaacgctggg ggccagcacg
     9721 gatatcgcgc tgttgcagac cattattgtc ggagttatca acctcacctt caccgttctg
     9781 gcaattatga cggtggataa atttggtcgt aagccactgc aaattatcgg cgcgctcgga
     9841 atggcaatcg gtatgtttag cctcggtacc gcgttttaca ctcaggcacc gggtattgtg
     9901 gcgctactgt cgatgctgtt ctatgttgcc gcctttgcca tgtcctgggg tccggtatgc
     9961 tgggttctgc tgtcggaaat cttcccgaat gccattcgtg gtaaagcgct ggcaatcgcg
    10021 gtggcggccc agtggctggc gaactacttc gtctcctgga ccttcccgat gatggacaaa
    10081 aactcctggc tggtggccca tttccacaac ggtttctcct actggattta cgggtgtatg
    10141 ggcgttctgg cagcactatt tatgtggaaa tttgtcccgg aaaccaaagg taaaaccctt
    10201 gaggagctgg aagccctctg ggaaccggaa acgaagaaaa cacaacaaac tgctacgctg
    10261 taatcttcct gtccagcacg ccgcgccagt tcggcgtgct gtctttttac tcccgcttca
    10321 gccgtttcga attacacaac cacagagtga tcaccagtaa caggatcgca gccgagtaga
    10381 tcaacacatc cagtggcgat ttatgatcga cgatgatcaa gcgcacaatg gcggtgatcc
    10441 caatatagac aaagtaacgt agcgggaagt gaaaaccgga ctgaaagtat ttcacaatca
    10501 gcgcgataaa ttcgaaatag agaaagtaaa ccaccagtcc ttctaccagc tcatatttgc
    10561 tggtttgttc tggcgcgaac agcacatctg ccagatgcac cgtttctttg ccgaggaaga
    10621 caaccaaaat caggccgagg cacaacaggc caagattgag tacggtctgc aaaatggtgg
    10681 agataaactc cacgcgcgga cgagagagtg acgtcatagc agtacctcct tcacagtggc
    10741 gagcttcctg tataacgcaa aaatgtgacg gagatctata ttttttgtgt atgttttgtt
    10801 cagcttgatt aagtgggcat ctcccatcaa cgtcgcatct ggcaaaacac acagccccaa
    10861 aaaatcaccc attcaggtga ttcccagcca ccagcaatgc gcgaaaaatc gaccatgttc
    10921 ctctggaaca aatgaaacgt aacctgataa aggtggtaaa aatgaagcct tattttgctg
    10981 ctttgatgtt atcagtctct gttcttcctg cttatgctgg tccgctcggt actgctgata
    11041 aagccgacct accgcaaagc aatgtctcca gcccaatgat ggcccagtcg ctcaggcaac
    11101 cagatctgca accgatatcg acggacagga agacagaatg ttttcgcctt tacacaccag
    11161 atcgcaaacc gggggtgaac tgcgttcctg atggctcaac aggccattaa gaatgagggt
    11221 gccagtgatt gcgaaaactg gcacctttcc catgacttag cggaaattga cgctacggtc
    11281 gctggtcatg tcatacaact ggaacttacg cccaagttgc tgaccaccgt cacgcgtcag
    11341 cggcgtccag ccaatcgccg cacggctgcg tgtcgggcca gacgagaaga gatccaacgg
    11401 taccgagaca tacacgcctt tggtgaagtc cccttcgccg tactcctctt ttgaaacatt
    11461 agtgatcgtg gcataaccac ccaccaccac gccgctatca aagcgtttgg cgatctccag
    11521 cgtgccgcct ttatcccctg ccagatactg cccgacgctg gctttaacta acacatcctg
    11581 agcgaaagat ggcgtccagt aggcggtcag atgtccggtt ttcacgctgt agtcggtgaa
    11641 tttcatcata tcttttgcac tacgccagtc gcgctgttta acgtagttgg catccagacc
    11701 aaacgcccag ttgctgtcca gcgggcgata caacacttct gcccccgcac cgccaaacat
    11761 ggtttcgaga taaccaccgt agacctgacc gtagaagccg ttgcccagat gctggaagta
    11821 gttggcttgc aggttattca catagacatc gttctgcaca tactcgcgca catgggtacg
    11881 cacgcgcggt aagtgcgagt cctgcggagg attagtgtag ttaaacttgt cgtagttgtt
    11941 ggcaagattt gcaaacaggc tgccggtcgt cagcagatgg tccgtcagcc acaaatctgc
    12001 cgttcccatc acgcccagct gatacatgta aaagttttcc gggccaccga ccgactggtt
    12061 cagcaccgga tcgatatgga aatcaaagcg tgatttgtcg atataccatc cctgctcggt
    12121 ggactgcgga accactggct cgacgcgttt ttgcgccagc gtcgtttcgt ggcccaacgg
    12181 ctctccggcg agatgatttt tcaggctggc gacatcggtt tccgtcgtca cctgcggcat
    12241 gttaaggcga ttttccgtaa cgcggatcgt tttgatccca tccggcagat cattcatcac
    12301 gatccgatta gcacggatga tcccttcgcg cgaatcacga tatttcacct gctcgccggt
    12361 aacatacagc gtatcgcctt tcgcctggat ctgcggatcg gcaagtccgg cattgtattt
    12421 caacagcgtt aactgattcg ccaccaccga atgctgcaaa atggcatcct gcggctgcgg
    12481 ttgatattgc gggcgggcgt tatcgttgta agacgggcgc agatcgttaa agttggtgcg
    12541 cagcgtgacg ccaaacataa aggtgttgcc acgttcatag ctaaggttaa cgtcggccca
    12601 atcggtaacg cgataaatcg caccgacgtt aaacttactt ttttgctcca gcttcccggc
    12661 aaaatcctgc tgataattat tgccttcata ctccagtttc aggcgcagcg gttgccaggg
    12721 cgtctggtat tccacgccgc caaacagtga ggcaggaccg tggaacatct ggctaccgtc
    12781 gatagatccc gcctgtttgt agctgttatc gcgatagcaa tatttatcac tggctgaaca
    12841 gagcggattt tttacattac cgctggtgcc cagatatccc cagcccaggc cgagcgtaaa
    12901 atcgaacggc ccccaggctt tgctggcaac aagatattcc gcatcaaaca gccccgtacc
    12961 gccgatatcc cgcgcaccaa ccgccacttg cggcagccag taactctctt cccacaaacg
    13021 cagtttgaga tcgaaggctt tatctttata cgtttgatcg ccagagaacg cttcgacgct
    13081 gctgtactgc cgggtgcgca cgtcggtgta gcgcagcgtt gtttccagcc acgggaagag
    13141 ttgcactgaa gctgagtaat aacggtactg atcgttatcg cgatagttca gactcaactc
    13201 cccttcccgt gccatacgcg cggtgggagt ttgtaataat cctacgccac cgaaatccga
    13261 ctgcgacgga ccaatgggcg caggatatgt ttcgccgtag caagctgtgc taatgcccag
    13321 cgccagtaag ctaagcagat gtcttttttt cattattggg gtatccgctg cgtcagagtc
    13381 tgaagaatgt cggcgttaag ggcatcaggc gtctcactcc agacggagtc cgcgaggcca
    13441 acataaataa tgctgccggg catcggctct acgtgacgct tgttccagta agccaccggc
    13501 gctttttgcg tgcgtccgtc cgggtaaacc acccacgcgt agctgcgatc cgcaccgcta
    13561 agcaggttct gcccggagag atagctcgcc acgtcgcgac ctggagtgaa tggctgcttg
    13621 ccaggacggc tgataagccc gaacagcgtg accgtggacg gtggtggccc gacccacagc
    13681 gtgtagttgc cctgcaacgg tgggttaccg cgttcggcaa cgcggacgat atcgggatca
    13741 agattgattt tttgcctgcc cgtcactttc aacgcctgaa tttgctggcg taaggcgtta
    13801 atcgcggcgg catcgtcggc gctggaatct gcagccagtt ctgccagccg tgtcagcaac
    13861 gcctgctgct gccgcaatgc tgccgccgtt gccagctctt cgctaatcac cgcaccaggc
    13921 caccaactgt ttgccagccg cggctggcct accagatcga ttaaatactc tgcgcccgtc
    13981 agcgttttgg cctcactgct gccattgctg aataccttga cggttcccgc cgcgaagacc
    14041 gatgacgccc ccacactcaa aatcaatgcg acaatagttt gtttaatcat tgttttgccg
    14101 ccttgatgag agtggttttc accgggaaat aatccgcgcc gagatactgt tccgactggc
    14161 gaatttgccc ttcgctgtcg atccagtagc ggttatgcca gcgcgcctgg tcggtggaga
    14221 cttcttcgtc cagcacgcga accggggttt catcgctgcc gaccttgacg gtatcggtgc
    14281 catcccattt gaagactgag cgtgcggtgg cgtagcgtac ctgctggtac tcggtccagc
    14341 ccatcgtgcg cgtccatgtt gcaccatcaa caatttgtgc gggtttaatc agcgggtcgg
    14401 cggcaaggtt attcacttca atcaggttgt cgccgccaag caaagtcttc accagacgac
    14461 catgttgtat gacaagagtg gcttgatcct gagtgaccca tttttgttgt ccgtcttcag
    14521 cgaaggccag caccacaaac aattgcggcc cgccattaag ctgcatgtac tggctggcgt
    14581 agggcatatt ttgaatatca tcgtccgtca gctgtacgcc tggcgtgccg aacagactgt
    14641 cccacagtga attgcccagc tctttagtcg tggctgaaca ggcctgtaat agcaggcaga
    14701 taagaatgag tgcaggtcgc ttcacgactc ttctccagag gggcagaata accacaccgg
    14761 agtgtggtta tggtaaatcc cttcatactt caagccacag atgcaatgac tcaaattatt
    14821 ttggggatac atactttatt actgggtact ggtggtcgtg gttgtggtgg tcccggtatt
    14881 agaaccatca ccgccaccgg ttgccgcgag cgcgacaccc accgccgagc ttacggtgct
    14941 gacgctggtc gcggaggaac tccccgccga aaccgacgtt gctgccgacc ctgccgcttc
    15001 gcccacctgt acaggtgcag cccacgcaga agtcgccgca agcgcagata tggcaaaaat
    15061 gccatacaga acttttttca taacaatttc ccttcattga atgaatggag atttacccaa
    15121 aaacatttcg ggcgggatcg agtatacaca actaaagcat gcgaattacg gcgcggggaa
    15181 ttagagatgg ggttttagga taattggaat gaatatcaaa tataagctac aacttatata
    15241 taaataacat taaaaatcat aatgttaaaa taaaagtaaa agtaattaat ctgagtattt
    15301 tccgaaatca atcacgatta aaaatctatt ttaggattaa cctcacggta tgatttccgt
    15361 taaatcacag acaaggggat ttatctgata aaacaatgcc gacataacgt cggcattgtt
    15421 attaaggttt ttaaattaat cgcgccacgc tttatagcgg ttaatcagac cattggtcga
    15481 gctatcgtgg ctgctgattt ctttatcatc tttcagctct ggcagaatac ggtttgccag
    15541 ttgtttaccc agctccacgc cccactggtc gaaggtgaag atgttcagga tcacgccctg
    15601 agtaaagatt ttgtgctcat acagcgcaat caacgccccc aggctgaacg gagtgatttc
    15661 acgcagcagg atggagttgg tcgggcggtt accttcgaac actttgaacg gcaccacgta
    15721 gtcaagcgtt gccggatctt taccctgatc gcgatattcc tgctcaacca cttcgcggga
    15781 tttaccaaac gccagcgctt cagtctgagc aaagaagtta gacagcagtt tctggtggtg
    15841 atcagagagc gggttatggg tgatagccgg agcgatgaaa tcgcacggta ccattttggt
    15901 tccctggtgg atcagctggt agaacgcgtg ctgaccgtta gtgcctggtt caccccagat
    15961 aatcgggcca gtctggtaat ccacaacgtt accgttacgg tcaacatact taccgttgga
    16021 ctccatattg ccctgctgga agtacgccgc gaaacggtgc atatactggt catacggcag
    16081 aatcgcctca gtttccgcac caaagaaatt gttgtaccag atgccaatca gcgccagcag
    16141 tacaggcagg tttttctcgg caggcgtggt ggagaaatgc ttgtccatcg cgtgtgcgcc
    16201 agaaagcagt tcaacgaagt tatcaaagcc gatggagaga acaatcgaca ggccaatcgc
    16261 tgaccacaaa gagtaacggc cgccgaccca gtcccagaac tcgaacatgt tggcagtatc
    16321 aataccaaac tcgccaacgg ctttggcatt ggttgagagc gccgcaaagt gtttcgcaac
    16381 gtgtttttca tcacctgccg ctttcaggaa ccagtcacgc gcgctatggg cgttggtcat
    16441 ggtttcctga gtggtgaagg ttttagatgc taccaggaac agcgtggttt ccgggtttac
    16501 ttttttcagc acttccgcga tgtgagtccc atcgacgtta gaaacaaagt gcatgttcag
    16561 gtggtttttg tacggacgca gtgcttcggt caccatgtac gggccgaggt cagaaccgcc
    16621 gatcccgatg ttcactacgt cagtgattgc tttgccggta taacccttcc actcaccgga
    16681 gataatcgct tccgagaagg ttttcatctt ctccagcacc gcgttgactt ccggcattac
    16741 gtctttgcca tcaaccaaaa tcggggtatt gctacggtta cgcagcgcta cgtgcagcac
    16801 agcgcggttt tcagtgcggt tgatcttctc gccagagaac atcgacttaa tcgcgcccgc
    16861 cagatcgcac tctttcgcca gatcttgtaa tttcgccagc gtctcttcag tgatgcggtt
    16921 tttggagtaa tccaccagca tctgatcgcc gaaggttgcg gagaacttag aaaaacgatc
    16981 gccgtcttta gcaaaaagat cggcgatcgt aacgtctttc atttcatcga agtgtttctg
    17041 tagtgcctgc caggcagcgg tctgcgttgg attgatgttt ttcattagca atactcttct
    17101 gattttgaga attgtgactt tggaagattg tagcgccagt cacagaaaaa tgtgatggtt
    17161 ttagtgccgt tagcgtaatg ttgagtgtaa acccttagcg cagtgaagca tttattagct
    17221 gaactactga ccgccaggag tggatgaaaa atccgcatga ccccgtcgtt gacaaccgcc
    17281 ccgctcaccc tttatttata aatgtactac ctgcgctagc gcaggccaga agaggcgcgt
    17341 tgcccaagta acggtgttgg aggagccagt cctgtgataa cacctgaggg ggtgcatcgc
    17401 cgaggtgatt gaacggctgg ccacgttcat catcggctac aggggctgaa tcccctgggt
    17461 tgtcaccaga agcgttcgca gtcgggcgtt tcgcaagtgg tggagcactt ctgggtgaaa
    17521 atagtagcga agtatcgctc tgcgcccacc cgtcttccgc tcttcccttg tgccaaggct
    17581 gaaaatggat cccctgacac gaggtagtta tgtctgaaat tgttgtctcc aaatttggcg
    17641 gtaccagcgt agctgatttt gacgccatga accgcagcgc tgatattgtg ctttctgatg
    17701 ccaacgtgcg tttagtagtc ctctcggctt ctgctggtat cactaatctg ctggtcgctt
    17761 tagcggaagg gctggaacct ggcgagcgat tcgaaaaact cgacgctatt cgcaacatcc
    17821 agtttgccat tctggaacgt ctgcgttacc cgaacgttat ccgtgaagag attgaacgtc
    17881 tgctggagaa cattactgtt ctggcagaag cggcggcgct ggcaacgtct ccggcgctga
    17941 cagatgagct ggtcagccac ggcgagctga tgtcgaccct gctgtttgtc gaaatcctgc
    18001 gcgaacgcga tgttcaggca cagtggtttg atgtacgtaa agtgatgcgt accaacgacc
    18061 gatttggtcg tgcagagcca gatgtagccg cgctggcgga actggccgcg ctgcagctgc
    18121 tcccacgcct caatgacggc ttagtgatca cccagggatt tatcggtagc gaaaataaag
    18181 gtcgtacaac gacgcttggc cgtggaggca gcgattatac ggcagccttg ctggcggagg
    18241 ctttacacgc atctcgtgtt gatatctgga ccgacgtccc gggcatctac accaccgatc
    18301 cacgcgtagt ttccgcagca aaacgcattg atgaaatcgc gtttgccgaa gcggcagaga
    18361 tggcaacttt tggtgcaaaa gtactgcatc cggcaacgtt gctacccgca gtacgcagcg
    18421 atatcccggt ctttgtcggc tccagcaaag acccacgcgc aggtggtacg ctggtgtgca
    18481 ataaaactga aaatccgccg ctgttccgcg cgctggcgct tcgtcgcaat cagactctgc
    18541 tcactttgca cagcctgaat atgctgcatt ctcgcggctt cctcgcggaa gttttcggca
    18601 tcctcgcgcg gcataatatt tcggtagact taatcaccac gtcagaagtg agcgtgacat
    18661 taacccttga taccaccggt tcaacctcca ctggcgatac gttgctgacg caatctctgc
    18721 tgatggagct ttccgcactg tgccgggtgg aagtagaaga aggtctggcg ctggtcgcgt
    18781 tgattggcaa tgacctgtca aaagcctgcg gcgttggcaa agaggtattc ggcgtactgg
    18841 aaccgttcaa cattcgcatg atttgttacg gcgcatccag ccataacctg tgcttcctgg
    18901 tgcccggcga agatgccgag caggtggtgc agaagctgca ttttaatttg tttgagtaat
    18961 atttccctac tggcgtataa cgctcaccga ttgttatacg ccatcctcac tgaaaacata
    19021 gattaaataa tatctgaaaa gcaaaatcac tcctgtagaa cagtattata atcaccgaaa
    19081 aatatcattt aacaggagtg ttaatggcta ctttaatgga aaaagatgca cttttaaacg
    19141 gagccagtca gtgcatcgcg tttttatcaa atatcattga taattgctca gtttcgtcgc
    19201 atcaggattc aggcgatgct ctcaagcgat tagtcagcta tcgcgattat ctttattcta
    19261 cccccgctga acttgttgat tttacacaag gtaagattct ccttcagcag gtaagaactc
    19321 aatatcagca tgaattcaac aatactacgc attcagaaaa taaagcatct tttgattcta
    19381 tttggcagcg tctgacaaat catgaagtca caccgcaaca gcatcccatt ggttttgttt
    19441 taggtgggca acccggagca ggtaaatcat ctctgattga attagccaaa agagagacaa
    19501 aagataatat aatgattatt aacggtgatg attttcgatt tctacaccct gattttaatt
    19561 atatctacca aaattatggg gatgattttg ttacccatac agcgaaattt tccggcgaaa
    19621 cggtcgaacg tgcaattgag agagcgatag tcagtaaact caacattgtt gtcgaaggaa
    19681 catttcgtaa tgccgcgacg ccgttacaaa cattaaaaaa actcaaagat gcaggctacc
    19741 aaacagaagt catgataaaa acaacctcag cggcattaag ctgggaaagc accaatgaac
    19801 ggtatagtaa ggacaaagag gcgggaaata ttgcccgaaa agtggataaa aaccaccatg
    19861 atatcgtcac cggtttgctg gcagaaaatg cccgcaaggt gtttgcttcg aacctgtcag
    19921 ataagtttgc ggtgtattcg cgtgagaaga tgatatttag ttcacaagcg gcaacgaatg
    19981 acgatattgc aacacttata cagaatgaaa tcagcgggaa tacacaataa taataagagc
    20041 tgatggcgtg aaataacgcc gggctttatg cccggcgcat taccgtctgg tacattaagc
    20101 tatccagttc cttaacgtcc ccttctctgc tatccggtaa cagcggtgga atttccccac
    20161 gcgccagttc cgcacgaata aacgcatgta acaatggacg gcgcggacca tattccgctt
    20221 ctcctgcgcg ctgtttacgt tccagtaatt cgtctatttc tgcacgtaac ggcgcatcaa
    20281 gttcgcttcc cgccagtaac tcagcaaagc gcattggcgg tacgccttta cccgcttcga
    20341 cccagcgcac ggccagcagc ggacgcagca cgtaaaagta ttttttcagt cgaacttcgt
    20401 cgccctgcaa atatccgggg aagtttttct gcgccatcga atagtaatgc cagcgcgcac
    20461 gcagcggaga aaaccacatt gggaccattg ccttaaacgc cgttatcgtt tcttcatctt
    20521 gttgataaat caccggagaa tccagccatt ctatcagcgt cgggttcgcg cctttcaata
    20581 atcccagcgc cttacgccac tcccaaccgg aaacatctaa ctcgtcgtcg atgggcagct
    20641 caataacatc gcgcggtgac tctactcgca gataccattc cagcgggtga acatacaaaa
    20701 accgtacatc gtagtcgctg tcgggcgagg caaatcccca gccgcggctc cctgattcac
    20761 aggcgtacag tactttcaca tcataccgcc gttcaatttc ttttaactgc tgcgataccc
    20821 tttcgcgcat cgcagcactg actccattta atggcatgtt actcaccctt tgatattaac
    20881 caacccatgc agaacctgat ccagaccgcc aaatacagag atgttatcga tccgctcagc
    20941 cactctctcc agcgtttcta gctcttttaa acgcaacgct accggattgt tctccatgac
    21001 tttggcggtg ttcagtaacg agcgcgtcgc tgccgtttct tcacgtcggc gaatcacatt
    21061 cgcctgcgca gatttctctg cctcaaccag ctgcgcgagg atatttttca tatcaccagg
    21121 caggacgata tctttcaccc ctaatgaggc aatctccata ccaaatggca acatgcgcga
    21181 tttcacctgt tcgctgacaa cgtcatcaat gacctgttta tcttccagca gttcatccag
    21241 cgtacgagta cctaccgctt cacgcagggc aaattgcaat tcccgataca aatgatcaat
    21301 cggcttcgtt aactgactaa atgccagcaa cacgtcgctg tagcgccagt tagcggcgag
    21361 gttaatccgc agattgacct tatctttcgt taagatttct tgcccgctga cttccagtac
    21421 ctgcaagcgg gtgtcaacca cttcagcgtc aaccagatgg ttaattttcc agtatgcggt
    21481 cagccccggc ggtaacagcg cctgcgtttc accatcgatt ttcagcacgc ctacatgcca
    21541 ggccggaacc tgcaccgtta gtattgcatc gcggccttta actgcccctt tgcgccgtgg
    21601 ttgcaatacc gcattcatca cctccgtttg tacctggact tgctgcgtat tcatccgcac
    21661 cagtgtcaaa tcatcctcta cacgccagta caaacggcgc gttgatggcg gcaggatctc
    21721 ctgcaaaata ccgtccatat acaacgcccc ggcttcaatc tccgacaagt ctgccaccag
    21781 gcaatatttc tccacccaat caggctgaaa gcggcgcaaa taatccgcca gcacgtcggg
    21841 aacttcgctg ccatcgagcg taatcagcaa gacttctggc gtatttaacc agggtaaaag
    21901 atgctcgcct gcatgtagca ctttgtagta atcgccgttt ctggagagta atgccagctg
    21961 atcttttctt acgcttattt ttttaatcat tatctattcc ttgcttaaca gccgtcgcgg
    22021 aagcagacac gaagcgacag gggaattccc ttttgcctgt tacccggcat ctttatcggc
    22081 gacgttatcg gggatcgctg actgcgcaat ccctcacttt ttatttaata agttaaaacc
    22141 agtattcatt tcaggaatga atgtgggaat cgaacccata attggcgtca aaggctgacg
    22201 gaacacgccc ggaaaacagc ctggccgttc ttggtgtgcc gacggggttt gacccccttt
    22261 gtaattcagt ttc
//
LOCUS       DAZFQD010000080        22229 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.080, whole genome shotgun sequence.
ACCESSION   DAZFQD010000080 DAZFQD010000000
VERSION     DAZFQD010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22229
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.080"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            364..4356
                     /locus_tag="ACHKTW_003075"
     CDS             364..4356
                     /locus_tag="ACHKTW_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S6 family peptidase"
                     /protein_id="HFO6464050.1"
                     /translation="MNKIYSLRVNDRKELVAVPETVGGRKKSSGIPGAGMLSRLLLAS
                     GAVAGVLFSSPALASVVGNTLPWQTYRDFAENKGAFHAGATNIPLYGKNGAVSGRLDK
                     APMMDFSVVDQILGVATLISPQYVAGVKHNGSYNTVRFGYADDTTYRLVDRNEHWRDF
                     HTPRLNKLVTEVAPVSVTDAGTGKGVYQNRSRYPVFYRMGSGTQYTGAASGALTRIAD
                     AYVWRTGGTVGSPVISDWSLVSNPGNLYQGVNGPLASYGTSGDSGSPLFAWDAVKKQW
                     VFVAVLHGYAGENGKTNWFTVIPAGDVNNTIKQDNSGTVVPAVAGGDIVWKYSKGSGE
                     GTLSQGGQVWKMNGFRGGSLNDGKDITFGGKGTVVLKDDVVQGAGSLTFNGDYTVRPE
                     GNQTWVGGGIIVNDGHRVDWMVNGLAGDALHKIGKGILVVAGSGENPGALNTGDGTVI
                     LAQKADAAGKVRAFSEVSIVSGRPVVVLQDSHQIEGDRIRWGYRGGTLDINGNDMAFN
                     RLAAADEGAVLTSRERPATVRLDFSQSGQKAVMWHGHFIGNLSVLNNTSSAVDFIMDG
                     GADMSGSFTQQGGGLYIQGHPVVHAVSSEAVATALRKQGDNSVLTQPVSFAQKDWESR
                     TFSIGQLKLKGAAFSLSRNATLTGDIDADNATMVLGSDSLYLDMKDGTGSSSAPVKGT
                     SAVGGASGSSTFRGNVNMRHSALTVRDHFTGSITASDSRIAVSSENVRLEGHSRLTSS
                     ALTVSDGGRLHVKGGLETDGGVTLDRGILLVDGGSVRNDVYERLLAWSEERGGLNGSG
                     EYDFMTGAAGLLRSYVRGSAGNVNLQNAAWMMTGNSSVKHLESSGSALYFSRPGGEFH
                     TLTAGSMDISDSVLVMRTDLNHSDQLRVTESLRGKNNLLLVDFTERSDGQKALNIPLV
                     TAPAGTGADVFSVKTRDTGFSHITPVVRAEQGTGGTAWQLNVVQPETAAEPVVTRQDA
                     ETPNPVEAVSPLPSPVSAPSPAPEASVTDVLTGENAGESGEYRDETRWLLTGYRSTVN
                     TSAVRDAGMLMSMGHRNFINEVNNLNKRMGDLRDINGEAGAWARIMSGTGSAGGGFSD
                     NYTHVQVGADKKHELDGLDLFTGVTMTYTDSNAGSDTFSGKTKSVGAGLYASALFDSG
                     AYIDLIGKYVHHDNEYTATFAGLGTKDYGSHSWYAGAEVGYRYHVTEDAWIEPQAELV
                     YGAVSGKQFSWKDQGMNLTMKDKDFNPLIGRTGVDVGKSFSGKDWKVTARAGLGYQFD
                     LLANGETVLRDASGEKRIKGEKDGRMLMSVGLNAEVRDNIRFGLEFEKSAFGKYNVDN
                     AVNASFCYSF"
     gene            5084..5209
                     /locus_tag="ACHKTW_003076"
                     /pseudo
     CDS             5084..5209
                     /locus_tag="ACHKTW_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="OmpW family outer membrane protein"
     gene            complement(5269..5775)
                     /gene="yciE"
                     /locus_tag="ACHKTW_003077"
     CDS             complement(5269..5775)
                     /gene="yciE"
                     /locus_tag="ACHKTW_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFO6464051.1"
                     /translation="MNRIEHYHDWLRDAHAMEKQAESMLESMASRIDNYPELRARIEQ
                     HLSETKNQIVQLETILDRNDISRSVIKDSMSKMAALGQSIGGIFPSDEIVKGSISGYV
                     FEQFEIACYTSLLAAAKNAGDTASIPTIEAILNEEKQMADWLIQHIPQTTEKFLIRSE
                     TDGVEAKK"
     gene            complement(5821..6321)
                     /gene="yciF"
                     /locus_tag="ACHKTW_003078"
     CDS             complement(5821..6321)
                     /gene="yciF"
                     /locus_tag="ACHKTW_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFO6464052.1"
                     /translation="MNMKTIEDVFIHLLSDTYSAEKQLTRALAKLARATSNEKLSQAF
                     HAHLEETHGQIERIDQVVESESNLKIKRMKCVAMEGLIEEANEVIESTEKNEVRDAAL
                     IAAAQKVEHYEIASYGTLATLAEQLGYRKAAKLLKETLEEEKATDIKLTDLALNNVNK
                     KAENKA"
     gene            complement(6407..6586)
                     /gene="yciG"
                     /locus_tag="ACHKTW_003079"
     CDS             complement(6407..6586)
                     /gene="yciG"
                     /locus_tag="ACHKTW_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HFO6464053.1"
                     /translation="MAEHRGGSGNFAEDREKASDAGRKGGQHSGGNFKNDPQRASEAG
                     KKGGQQSGGNKSGKS"
     gene            complement(6967..7773)
                     /gene="trpA"
                     /locus_tag="ACHKTW_003080"
     CDS             complement(6967..7773)
                     /gene="trpA"
                     /locus_tag="ACHKTW_003080"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HFO6464054.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPIEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKVFVQPMKAATRS"
     gene            complement(7773..8966)
                     /gene="trpB"
                     /locus_tag="ACHKTW_003081"
     CDS             complement(7773..8966)
                     /gene="trpB"
                     /locus_tag="ACHKTW_003081"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HFO6464055.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPDKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            complement(8978..10339)
                     /gene="trpCF"
                     /locus_tag="ACHKTW_003082"
     CDS             complement(8978..10339)
                     /gene="trpCF"
                     /locus_tag="ACHKTW_003082"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HFO6464056.1"
                     /translation="MMQTVLAKIVADKAIWVETRKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVIRDDFDPARIAAIYKHYASAISVLTDEKYFQGSFDFLP
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDEQYRQLAAVAHSLEMG
                     VLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQL
                     HGNEEQLYIDTLREALPAHVAIWKALSVGETLPAREFQHVDKYVLDNGQGGTGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            complement(10340..11935)
                     /gene="trpD"
                     /locus_tag="ACHKTW_003083"
     CDS             complement(10340..11935)
                     /gene="trpD"
                     /locus_tag="ACHKTW_003083"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HFO6464057.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPANTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAD
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLHVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGEIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            complement(11935..13497)
                     /gene="trpE"
                     /locus_tag="ACHKTW_003084"
     CDS             complement(11935..13497)
                     /gene="trpE"
                     /locus_tag="ACHKTW_003084"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HFO6464058.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGDRPATLLLESADID
                     SKDDLKSLLLVDSALRITALGDTVTIQALSGNGEALLTLLDNALPAGVENEQLPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNELRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYVADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSIPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     misc_feature    complement(13519..13612)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            complement(13589..13633)
                     /gene="trpL"
                     /locus_tag="ACHKTW_003085"
     CDS             complement(13589..13633)
                     /gene="trpL"
                     /locus_tag="ACHKTW_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HFO6464059.1"
                     /translation="MKAIFVLKGWWRTS"
     gene            13771..14652
                     /gene="rnm"
                     /locus_tag="ACHKTW_003086"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
     CDS             13771..14652
                     /gene="rnm"
                     /locus_tag="ACHKTW_003086"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
                     /EC_number="3.1.13.-"
                     /EC_number="3.1.3.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase AM"
                     /protein_id="HFO6464060.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAIAPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQLIAERLEKAQIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLAALARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            14649..15269
                     /gene="yciO"
                     /locus_tag="ACHKTW_003087"
     CDS             14649..15269
                     /gene="yciO"
                     /locus_tag="ACHKTW_003087"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFO6464061.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            15370..16242
                     /gene="rluB"
                     /locus_tag="ACHKTW_003088"
     CDS             15370..16242
                     /gene="rluB"
                     /locus_tag="ACHKTW_003088"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HFO6464062.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGGRRSGGRNNG"
     gene            complement(16282..16872)
                     /gene="cobO"
                     /locus_tag="ACHKTW_003089"
     CDS             complement(16282..16872)
                     /gene="cobO"
                     /locus_tag="ACHKTW_003089"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HFO6464063.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDMVLLDELTYMVAYDYLPLEEVVQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPVKHAFDAGVKAQIGIDY"
     gene            complement(16869..17627)
                     /gene="yciK"
                     /locus_tag="ACHKTW_003090"
     CDS             complement(16869..17627)
                     /gene="yciK"
                     /locus_tag="ACHKTW_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HFO6464064.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSENCQQLAQRIVVNYPRLDGVLHNAGL
                     LGDICPMSEQNPQVWQDVMQVNVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            17847..18896
                     /gene="sohB"
                     /locus_tag="ACHKTW_003091"
     CDS             17847..18896
                     /gene="sohB"
                     /locus_tag="ACHKTW_003091"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HFO6464065.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            complement(18932..19183)
                     /gene="yciN"
                     /locus_tag="ACHKTW_003092"
     CDS             complement(18932..19183)
                     /gene="yciN"
                     /locus_tag="ACHKTW_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HFO6464066.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVMQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            19563..22160
                     /gene="topA"
                     /locus_tag="ACHKTW_003093"
     CDS             19563..22160
                     /gene="topA"
                     /locus_tag="ACHKTW_003093"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFO6464067.1"
                     /translation="MGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKVRYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVNKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
BASE COUNT         5408 a         5348 c         6059 g         5414 t
ORIGIN      
        1 atattcgtga agtcgggtaa tgagagtgga acggtggcag tgagaatgga acagaaaaaa
       61 atcagatgcg gaaagcggaa atacacagcg ggtgcgatgt gcttcatgct gtttgtatgg
      121 ctggggggtg tggtgacatt ctgtgtgctg gccgctctga taatgaggtg aaaacggaag
      181 agagtttact tctttaattc gaactttctg ataactgaga acttgttaaa tgctcgtggg
      241 tttatatatt taaatcaatt tgttaatgtt aatttcatct ttatcagtca aagtttgttt
      301 tagtatgatg attgtgccca ctgtttattg ttgtccggga gaaaatacag ggaatcccag
      361 ggaatgaaca aaatatactc actcagagtt aatgacagaa aagaactggt tgctgtaccg
      421 gaaactgtgg ggggcaggaa aaagtcgtcc ggcattcccg gtgcggggat gctttcccgt
      481 cttcttctgg cctcaggagc cgttgccggc gttctgtttt cttctcctgc gctggcatcc
      541 gttgtcggga atactctgcc atggcagacc tacagggatt ttgctgaaaa caagggggcg
      601 tttcatgccg gggccacaaa tattccgctt tatggtaaga atggggctgt cagtgggcgg
      661 ctggacaagg ctccgatgat ggatttcagt gttgtggacc agatactggg ggtggcaaca
      721 ctgatttctc cgcagtatgt ggcgggggtt aagcataacg gcagctacaa tacggtgcgt
      781 tttggttatg cggacgatac gacatatcgc cttgttgacc gtaatgagca ctggcgtgat
      841 tttcatactc cccggctgaa caaactggtg acagaagttg ccccggtgag cgtcacggat
      901 gctggaaccg gcaagggggt ataccagaac cgcagtcgtt atccggtgtt ttatcgtatg
      961 gggagtggaa cgcagtacac cggagctgct tccggtgcgc tgaccagaat agcggatgct
     1021 tacgtctgga ggactggcgg gacggtggga agtccggtca tttctgactg gagtctggtg
     1081 tcaaacccgg gaaatcttta ccagggtgtt aatggtccgc tggcgtctta cgggacatca
     1141 ggagacagtg gctcaccgct ttttgcctgg gatgcggtga agaagcagtg ggtatttgtt
     1201 gctgtacttc atggttatgc cggtgagaac gggaagacga actggtttac ggtgattcct
     1261 gcaggggatg tgaataacac gataaagcag gacaattcag gcacagttgt gccggcagtt
     1321 gccggtggtg acattgtgtg gaagtacagt aagggaagcg gagagggaac cctttctcag
     1381 ggcgggcagg tatggaagat gaacggcttc aggggaggga gtctgaatga cgggaaagat
     1441 atcacctttg ggggtaaggg cacggttgtt ctgaaggatg atgttgtcca gggggctggt
     1501 tcgctcacgt ttaatggtga ttatacggtc agaccggaag ggaatcagac ctgggtgggc
     1561 gggggaatca tcgtgaacga cgggcaccgt gttgactgga tggtgaacgg acttgcggga
     1621 gacgcacttc ataaaattgg caagggaata ctggtggtgg ctggcagtgg tgaaaatccc
     1681 ggcgccctga atacggggga cggaacggtc attctggcac agaaggctga tgctgcagga
     1741 aaggtccggg cattcagtga agtcagtatt gtcagcggac gaccagttgt ggtgctgcag
     1801 gacagtcatc agattgaagg ggaccgcatt cgctggggat accggggagg cacgcttgat
     1861 atcaacggaa atgacatggc cttcaaccgc ctggctgcgg ctgatgaggg ggcagtactc
     1921 acaagccggg agcgtcctgc cacagtccgt ctggatttca gccagtcagg gcagaaggcc
     1981 gtgatgtggc atggtcattt tatcggtaac ctcagcgtcc tgaacaacac gtcatctgcg
     2041 gtggatttta tcatggatgg cggagcggat atgtccggca gttttacaca gcagggcggg
     2101 ggactgtata tccaggggca tccggttgtt catgctgtca gcagtgaggc ggttgcgact
     2161 gcattgcgaa agcagggtga taattctgtt ctgacgcagc cggtatcatt tgctcagaag
     2221 gactgggaga gcagaacgtt cagcatcggt caactgaaac tgaagggggc ggcattcagc
     2281 ctttcccgca atgcgacact gacgggagat attgatgctg ataatgccac catggtgctg
     2341 ggaagtgact cgctatatct ggatatgaag gacggcacgg ggagcagcag tgcgccggtt
     2401 aaggggacgt ctgccgtggg cggtgcatcc ggtagcagta catttcgcgg gaatgtgaat
     2461 atgcgtcatt ctgccctgac ggtccgcgac cattttactg gcagtataac ggcatcggac
     2521 agccggatag ctgttagttc agagaatgtg cgtctggaag ggcattcccg cctgacctct
     2581 tctgcactta ctgtcagtga cggcgggcgt ttacatgtga aggggggact ggagacagat
     2641 ggtggtgtga cactggacag aggaatcctt ttggtggacg ggggaagtgt caggaatgat
     2701 gtgtatgaac gtctgctggc ctggtcagag gagcgtggcg ggctgaacgg aagcggtgag
     2761 tatgatttta tgacgggggc agctggtctc ctgcggagtt atgtcagagg gagtgcaggt
     2821 aatgtgaatc tgcagaatgc ggcctggatg atgacgggaa actcatccgt gaagcatctt
     2881 gagtcatcgg gaagtgctct gtatttcagc aggccgggcg gggagttcca taccctgacg
     2941 gcaggcagta tggatatcag cgacagtgtt ctggtgatgc ggacggacct taaccactca
     3001 gaccagcttc gggtgacaga atcgctgcgg ggtaagaata acctgttgtt ggttgatttt
     3061 actgagcgtt ctgacgggca gaaggcgctg aatatccctc tggtgacggc gccggccggt
     3121 accggtgcgg atgtgttcag tgtgaaaacg cgtgataccg gcttcagtca tatcacacct
     3181 gttgtcaggg ctgaacaggg gacgggcggc acggcctggc agctgaatgt tgtgcagccg
     3241 gaaactgccg cagagcctgt ggtgacgcgg caggatgcag agacgccgaa tcctgtggaa
     3301 gctgtatcac cgctcccttc accggtttct gctccgtcac cggctccgga ggcgtcagtg
     3361 acggatgtac tgaccggaga gaatgcgggg gaatcaggtg agtaccgtga tgagacccgg
     3421 tggctgctga caggataccg gagtacggtt aatacctccg cggtcaggga tgcggggatg
     3481 ctgatgtcaa tggggcaccg taacttcatt aatgaggtca acaacctgaa caaacgtatg
     3541 ggtgacctgc gggacatcaa cggcgaggcc ggtgcatggg cccgtatcat gagcggaacc
     3601 ggttctgccg gcggaggctt cagtgacaac tacacgcacg ttcaggtggg tgcggataaa
     3661 aaacatgaac tggacggtct ggacctcttc accggcgtga ccatgacata taccgacagc
     3721 aacgccggca gcgacacctt cagtggtaaa acgaagtcag tgggggcggg tctgtatgct
     3781 tcagccctgt ttgattccgg ggcctatatc gacctgattg gtaagtatgt tcaccatgat
     3841 aatgagtata cggcgacctt tgccggactt ggcacaaaag actacggctc tcattcctgg
     3901 tatgccggtg ctgaagtggg ttaccgctat catgtcacag aggatgcctg gattgagcca
     3961 caggcggaac tggtctatgg tgctgtctcc gggaaacagt tcagctggaa ggaccaggga
     4021 atgaacctca ccatgaagga taaggacttt aatccgctga ttggccgtac cggtgtggat
     4081 gtgggtaaat ctttctccgg taaggactgg aaagtgacgg cacgtgccgg tcttggctac
     4141 cagtttgacc tgctggcgaa cggagaaacg gtactgcgtg atgcatccgg agagaagcgt
     4201 attaagggcg agaaagacgg ccgtatgctg atgtctgtgg gtctgaacgc agaggtccgt
     4261 gacaacatcc gttttggtct ggagtttgag aaatcagcct ttggtaagta caacgttgat
     4321 aatgctgtca acgccagctt ctgttattca ttctgacggt tgttactgaa ggggattatg
     4381 gcctgacggc tccctgtaaa taaagacagg cacagaagat tgtgagttac tcagtgttcc
     4441 accaaaaagt aaggtcgcaa ctctgtgcct gcttcgtaag tgtacagtga gggccatatg
     4501 gttataactg aagcatccga cgacgtggtg tctacccaaa aagcgacctg tgaatattag
     4561 gctggttaag tgcgtatttt catggaagga gatcacccaa taaccccgat ctcctttatg
     4621 ttaatacgca ttaagcgggt aaaacaggaa gattaagaaa gggagagttt ttcgactccc
     4681 caaccaagag aagcagctta taactaccag atagttgtta tgtcatacat gacggataat
     4741 gtcataaaaa atatgtcatt gccgtgatta tgatcgcgtt attggataaa gcagttcagc
     4801 ggtttcccag gcttcctgca gcgtttcctg tacaaattcg tgcgcggatt ctttatttac
     4861 aactcttaaa atggtgagtc catcattgct ggtggttctg acaatcacgt ccacatggtc
     4921 atatccgtta gttatggaat gactataagg ggggataagc ctctgcaaaa caaaaaaaga
     4981 ctttaacaaa atcaatcagt taaaaacaca gaaaaacggt atgaaaaatc accataaaag
     5041 tttgtttttc cttttataaa tagctgttta tcacattttt tataatctac cagtctggta
     5101 catggatatc gataccaccg ccaattataa gctaggcggt gcacagcaac acgatagcgt
     5161 acgcctcgat ccgtgggtgt ttatgttctc agcaggatat cgtttttaat tccacaaaaa
     5221 aacgaccccg taatatacgg ggtcaataag gacatggcat aaagtgtatt atttcttcgc
     5281 ttctacgccg tcagtttcag agcgaattaa aaatttctca gttgtttgcg gaatatgctg
     5341 aatcagccag tcggccattt gcttttcctc atttaaaatc gcttcgatgg ttggaattga
     5401 agctgtatca ccggcatttt ttgctgctgc taatagtgag gtgtaacagg cgatttcaaa
     5461 ttgctcgaag acatatccgc taatagagcc tttgactatt tcatcagaag ggaatatacc
     5521 accgattgac tgcccaagcg cagccatttt actcatggaa tctttaatga ctgaacgtga
     5581 aatgtcatta cgatcaagaa tagtttccaa ttgaacaatc tggtttttgg tttcactaag
     5641 atgttgttca atacgagcgc gtagttcagg ataattatct atacggctgg ccatggattc
     5701 aagcatagat tcggcttgct tttccattgc gtgggcgtca cgtaaccagt catgataatg
     5761 ttcaatacga ttcatgaaaa tgtcctcttt ataaaatgac taaaagttaa agttcatatt
     5821 tcaggcttta ttttcggctt tcttatttac gttattaagg gccagatcag tcagtttgat
     5881 gtcggtggcc ttttcttctt ccagggtctc tttcagaagc ttcgctgctt tacgataacc
     5941 taattgttca gccagcgtcg ctaatgtccc gtaactggca atctcataat gctcgacttt
     6001 ctgtgctgcg gcaatcagtg cggcatcacg cacttcgttt ttctcggtac tttcgatgac
     6061 ctcattagct tcttcaataa gaccttccat tgccacacat ttcatgcgct taattttcag
     6121 attcgattct gattccacaa cttgatcaat acgttcaatc tgtccatgtg tttcctcgag
     6181 gtgcgcatga aaagcctgac ttaatttttc gtttgatgtt gctcttgcga gttttgccag
     6241 tgcccgggtt aattgttttt ctgcgctgta ggtatctgaa agcaggtgaa taaatacatc
     6301 ttcaatggtc ttcatattca tatttttctc cagtgaaatc actgcgagcc atacggcccg
     6361 cagatgcaat tatctttatc agctgatatt aataattaac ggtgaatcag gatttgcctg
     6421 atttattacc accgctttgt tgaccgcctt ttttacccgc ttcagatgcg cgttgcggat
     6481 catttttaaa attaccgccg ctatgctgac cgcctttacg gcctgcgtcg gatgccttct
     6541 cacggtcttc ggcgaaattt cctgaaccac cacgatgttc ggccatgtta tttctcccgt
     6601 tgcgttgcat tgtttcatta atatgagtgt tgtgtgtcga cactcattaa aattagtcgc
     6661 taatgagaat tagtcaaatt aagcgcaacg agaagataga gggaaaatat attttgagga
     6721 acattctgga tatattaaca attacctgag gaataagtga cttagagaaa attattgatt
     6781 ttactggtgt tatgttgcgg gattaatttg tttgattata ttactgttgg gcggaaaaat
     6841 gacgtaagtt gaagttcgac cggggtaagc gaaacggtaa aaagataaat attaaatgaa
     6901 tttaggattt ttccggcttc attaaagaaa gttaaaatgc cgccagcgga actggcggct
     6961 gtgggattaa ctgcgcgtcg ccgctttcat cggttgtaca aaaactttca gtgccgccag
     7021 cattttctct ggctcattaa tatgttgctc gatgatttta acaatggccg aaccagaaat
     7081 cgcgcccgca gctcctgcat caatcgctgc ttttacctga tccggggcgg aaataccaaa
     7141 cccctgcaat ggaggtgcag cgttgtactc tttcagcttc gcaaccagat gattgagggg
     7201 taacgcggcg cggttttctg cgccggtcac gcccgctcgt gacagcaaat aggtgtaacc
     7261 acgaccgtaa gaggctatct ggcgcagcag gtcgtcatcg gcatttggcg ggcagatgaa
     7321 gataggtgcg acattatggc gcaacgcggc ctggcggaag ggcgcggact cttcaattgg
     7381 cacatcggca accagcaccg aatcgacgcc gactttctcg cactgggcat aaaactcatc
     7441 aatgcctttg ttaaacacca gattggcata catcaacagg ccaatgggaa tggtcgggtg
     7501 tttctggcga atcagtgcca gcatttcaaa acattgtgcc ggagtcacac ctgccgcaaa
     7561 ggcgcgcaga gtggcgtttt gaatggtcgg gccatccgcc agtgggtcgg agaaggggat
     7621 acctaactcc agcgcgtcag caccggcttc aattagcgta tcgataattt tcaatgactg
     7681 ctcaatgccc ggatcaccga gcgtgacgaa aggaacgaat gcgccttctt tgcgctcctt
     7741 caactgggca aacagagatt cgtagcgttc catcagattt cccctcgtgc tttcaaaata
     7801 tcgtgaacgg tgaagatgtc tttatcgccg cgaccggaaa ggttaaccac cagtagctgc
     7861 tctttatccg ggttttcgcg catcattttc aacgcatggg ccagggcgtg ggaggattcc
     7921 agcgccggga tgatcccttc gtgcaggcac agcgttttga aggcttcaag ggcttcatca
     7981 tcggtaatag acacgtaatc agcgcgtcca gtgctgttaa gatacgcgtg ttgtgggccg
     8041 acagacggga aatccagtcc ggcggagatg gagtaagatt cttcaatctg cccgtcttcg
     8101 gtttgcatca tcggcgcttt cataccgaaa tagataccca cgcgaccatg ttttagcggt
     8161 gcgccgtgct cgccagtttc gataccgtga ccacctggct ccacaccaat caggccgacg
     8221 ttggtttcat tgatgaaatc agcaaacatg ccgatggcat tcgaaccgcc gccaacacag
     8281 gcgataacgg catccggcag gcgaccttct ctttccagaa tctgcgcttt ggtttcttcg
     8341 ccaatcatcc gctgaaactc acgcacaatg gtcggataag gatgcgggcc agctgcggtg
     8401 cccagcatat agtgcgcggt ttcgtaacta ccggaccagt cgcgcagcgc ctcgttacag
     8461 gcatctttca gcgtcgcgga accgctatgc accgggatca cttccgcacc cattaagcgc
     8521 atacgaaaaa cgttaggcga ctggcgttca acgtctttgg cacccatata aatacggcat
     8581 ttcaggccga gcagggcgct ggcaagggcc gacgccacgc catgctgacc ggcaccggtt
     8641 tcggcgatga tttcggtttt acccatccgc ttcgccagca acgcctgccc cagcacctgg
     8701 ttagttttat gcgcgccgcc gtgcagcaaa tcttcacgct tgagatacag cgtggtgttc
     8761 gtcccggctg taatgttctg gcatttggtc agcgcggttg gacgcccggc atagtttttc
     8821 agcaggtcgt tgaactgagc ctgaaattca ggatcttttt gcgcactgac aaaagcttct
     8881 tccagctggc gcagagcagg catcaggatt tgtggcacgt acatgccgcc aaactcacca
     8941 aaataggggt taagtaatgt tgtcattgtt cctttcctta atatgcgcgc agcgtctgga
     9001 aaaccgaggc caaaagacgt gcgtctttga tgcccggttg cgactctaca gcagaattaa
     9061 aatcaagtcc ggcgcagccg gtttgtgccg cttccacgca gttatctgcg cctaagcccc
     9121 ccgccagcag aacgttgcca agcgattgac catttaatag tgaccagtca aaacgttgcc
     9181 cggttccacc ctggccgttg tctaaaacat atttatcaac gtgctgaaac tcgcgggcgg
     9241 gcagggtttc accgacgctt aatgctttcc agatggcaac atgtgctggc agagcttcac
     9301 gcagcgtatc gatatacagc tgttcttcat taccatgcag ttgcactgcc gccagcgata
     9361 acaccttagc tttgtccacc acatcggcaa tatcgtgatt gcggaacacg ccaacatact
     9421 gcaacggtgc cgcagccatc acttcctgcg cctgttcaac gttgacgcaa cgcggtgatg
     9481 tcgcaacaaa aatcaaccca ccgtaaatcg cgcccgcgtc ataagctgct ttagcatctt
     9541 gcccacgcgt caggccacat actttattct cacccagcaa cacccggcgc acggcggcgt
     9601 gcaaatcgtc atgggccatc aacgccgaac caatcagaaa accgttagcg aagtggctta
     9661 actcgcgcac ctgagcgtaa gtattgatgc cggattcgct gattaccgtc acgttgtgcc
     9721 ccagtttcgg cgcaagctcg cgggtacggt tgagatcaat cgacaaatca cgcagatcgc
     9781 ggttgttgat gccaacgacc tttgccccca atgcaatggc acgctccagt tcctcttcat
     9841 tactgacttc ggtcagcaca cccatctcca gactgtgggc gacggcggca agctggcgat
     9901 attgttcgtc atccagtact gaaagcatta ataagcaggc atcagcctgg taatagcgcg
     9961 ccagatagat ctggtaagga tcgataatga agtctttaca taaaatcggc tgcggggcga
    10021 tttggctgac gatggggagg aaatcaaagc tcccctgaaa atatttctca tcagtcagca
    10081 ccgaaattgc cgaagcgtaa tgtttataaa tggcggcaat gcgtgctgga tcgaaatcat
    10141 cacggatcac gccttttgac ggcgacgctt tcttgcactc cagaataaac gccgtgcgcg
    10201 caccctgtag cgcatcataa aaatgtcgcg tgctcggctg aacctcattc tgaaaactgg
    10261 ccagcggttg ctgctgtttg cgggtttcta cccaaatcgc cttgtctgcg acgattttcg
    10321 ctaaaacggt ttgcatcatt taccctcgtg ccgccagtgc ggtaactctg tcgtaagcgg
    10381 aaccactgcg cagtacctca agaacggttt gcgcattggc ttgcagatct tcatggccat
    10441 gcaggcgcat taacatggcg acgttcgcag cgacggctgc ttcatgggcg gcgtcgcctt
    10501 taccttgtaa caagcgtgtt aaaatgtcac ggttttcttc cggtgttccg cctgccagtt
    10561 gctcctggtg gtagggagtc aggccaaaat cttcagcggt caattgatag ctcttaattt
    10621 cgccgtcatg cagctcggca acgattgtcg gcgcgtgtaa tgaaacttca tccatcccgc
    10681 cgctgtgcac caccgccgcg cgttgatacc ccagcacgtg caaggtttcg gcaatcggca
    10741 gcaccagttc cggactataa acaccaatta acgccagcgg cggatgcgcc gggttaatca
    10801 atggccccag cacattgaac agggtgcggg ttttcagttg ctggcgaacc ggcatcgcgt
    10861 ggcggaatcc ggtgtgatac ttcggcgcaa agaggaaaca tacacctaac tcatccagcg
    10921 cctggcgcga tttatcggcg ttcatatcaa gattaatacc gaacgccgcc agcagatcgg
    10981 acgaaccaga tttactggag acgctacggt tgccgtgttt cgccactttc agcccacagg
    11041 ccgcggcgac aaacgcactg gcggtagaaa tattgatact gttgctgccg tcaccgccag
    11101 taccgacgat atcagcaaac agataatccg ggcgcgggaa cggcgcggcg ttttccagta
    11161 gcgcggttgc tgctccggcg atctcgttcg ggtgctcacc gcgaattttc atgctcacca
    11221 gcgccgccgc cagttgttcc ggcttcagct cgccacgcac caccgctgaa aacagctggt
    11281 ggctttcttg ttggctaagc gtctgcgcct gatacagttt ttccagaatc ggttgcagcg
    11341 tgttggctgg ctctagtttc tgctgcgccc aggccagcgt ttgttccagc aggcgagcgc
    11401 cctgggtggt gagaatggat tccggatgga actggaatcc acaaacgcga tccgcatcgt
    11461 gacgtactgc catcaccatg ccattaaaat gggcgttgat ggttaaaccg gccggaatgt
    11521 tactgccaac cagcgagtga taacgcgcca ccggcagcgg gtttgttaat ccggcaaaca
    11581 tcgcctgacc gtcatgttca atgctcgacg ctttaccgtg aagaatttcg cccgcctgac
    11641 cgacatagcc cccgtaagct tcgacaattg cctgatgtcc gaggcaaatg ccaataatgg
    11701 gcagcttgcc acgcaagcgg gtgaggagtt ccggcataca accggcttcg ctcggcacac
    11761 cggggccagg agaaagcatc agtaccggat tgctcatggt cgccaggcgt tcaattaagg
    11821 tctgcgccgg aatatggttg cggtaaatca ccacgttatg cccattgctg cgcaactgat
    11881 ctgccaggtt gtacgtaaaa gagtcgatat tatcgagcag cagtatgtca gccatcagaa
    11941 agtctcctgt gcatgatgcg cggtggcaat agcgcgcagt acagcgcggg ctttattacg
    12001 ggtttcgtcg gcttccgact gcggaataga atcaaggact acgccagcac cggcttgcac
    12061 ggtggcgata ccgttttcca ccagcgccga gcggatcaca atgcaggtgt cgagatcgcc
    12121 gtgcgcggtg aaataaccta ccgcgccgcc gtagctgccg cggcgacgac cttccgcctc
    12181 ggcaattaac tgcatggcgc gcactttcgg cgcaccgctt aacgtcccca tattcataca
    12241 ggcgcggtaa gcgtgtaggg cgtcgagatc gtggcgcagt tcaccgacca cgcgggagac
    12301 caggtgcatc acgtaagagt aacggtcaac tttggtgagg tcggcgacgt agcggctgcc
    12361 gggggtgcaa atgcgtgcca gatcattacg ggcaagatcc accagcatca gatgttcaga
    12421 aagctcttta tgatcggtac gcatttccag ttcgatgcgg ctgtcgaggt ctctgtccag
    12481 cgagccatcg gcacgacgac cgcgtggacg tgttccggca atcgggtaga tctcaatctg
    12541 gcggctggtg gcgtcatact tgagcgaact ttccggcgac gcgccaaaca gggtgaaatc
    12601 attatcctgc ataaaaaaca tgtacgggct gggattactc tttttcagca cgtaataggc
    12661 cgccagcggt gacgggcagg gcagagagaa acggcgagat ggcaccacct ggaaaatttc
    12721 tccagcgcga atcgcttttt gcaacaaacg cactacgcca ccgaactctt catcgctctg
    12781 attacattca caacgcatat gcggcacgga aaccaccggc agcggcggcg cggcttcggt
    12841 cagttgctga cgtagttcgt tcaggcgagc agtgagacgt tgtttttctt cttcattcgg
    12901 agcaaacagg ctggcctgaa tacgggtgct ttttttctga tggtcaatca ccatcagcgt
    12961 ttcagcgaga taaaaacaga aatcagggca gttattttcc gctgacagtt gcggtaaatc
    13021 ttcaaatccc gccacaaggt cataagagaa caggccgccg aagaacattg cttctcgttc
    13081 ttccttcggt acattcaaca gattctgcaa taaacggaaa gcgtcaaaaa ccgaaaggga
    13141 gcataagcga gcgtcttcat ccagcagtgg actgacaggg gggaagcgca gcacacggca
    13201 gtttggtaat tgttcatttt ccacacccgc aggcagggcg ttatccagta gtgtcagcag
    13261 ggcttcgccg ttgccggaaa gtgcctggat tgtgacagtg tcacctaaag ctgtaatgcg
    13321 cagcgcactg tctaccagca gcaggctttt taaatcatct ttgctgtcga tatctgcgga
    13381 ttccagcagc agcgttgccg gacgatcccc acacaactgg tgaaaaagcg cggtgggatt
    13441 gtcgcgataa gcgccttcgc aggttagcag ttcgagagtc ggtttttgtg tttgcattgt
    13501 tattctctaa ttttgttcaa aaaaaagccc gctcattagg cgggctgggt atctgattgc
    13561 tttacgcatg gtgaatacac tgcccgtttc aggaagtgcg ccaccaacct ttcagtacga
    13621 aaattgcttt cattgtcgat acccttttta cgtgaacttg cgtactagtt aactagtttg
    13681 atgattaatt gtcaacagct catttcagaa tatttgccag aaccgttatg atgtcggcgc
    13741 aaaaaacatt atccagaacg ggagtgcgcc ttgagcgaca cgaattatgc agtgatttac
    13801 gacctgcaca gccataccac agcttccgat ggctgcctga cgccagaagc attggtgcac
    13861 cgtgcagtcg agatgcgcgt cggcaccctg gcgatcaccg accatgacac cacagctgca
    13921 atcgcgccag caagagagga aatttcacgt tcgggactgg cgttgaatct tattcccggc
    13981 gtggaaattt ccacggtctg ggaaaatcat gaaattcata ttgtcgggct gaatattgat
    14041 attactcatc cgctaatgtg tgagttcctc gcgcagcaga cagaacggcg caatcagcgg
    14101 gcgcagctga ttgccgaaag acttgaaaaa gcgcaaatac ctggggcgct ggaaggcgca
    14161 caacgactgg cgcagggcgg cgcagtgacg cgcggtcatt ttgcgcgttt ccttgttgag
    14221 tgcggcaaag ccagttcaat ggcggatgtc tttaaaaagt atctggcgcg cgggaaaacc
    14281 ggatacgttc cgccacagtg gtgtacaata gaacaagcta ttgatgtcat tcatcattct
    14341 ggcggtaagg cggtgctagc tcatcctggg cggtacaatc ttagtgctaa atggctgaaa
    14401 agattggttg cgcattttgc cgaacaccac ggtgacgcga tggaagtcgc gcagtgccag
    14461 caatcgccca atgaacgtac ccagctggcg gcccttgcgc gtcagcatca tttatgggca
    14521 tcacaaggat ctgattttca tcagccatgc ccgtggatcg aactgggtcg taaactctgg
    14581 ctgcccgcag gcgttgaagg cgtctggcag ctatgggaac agccgcagaa caccacagag
    14641 agggaattat gagccagttt ttttatattc atcctgataa cccacagcaa cgtctgatca
    14701 accaggcggt ggagatcgtg cgtaaaggcg gggtgattgt ttatccaact gattccggct
    14761 atgcgctcgg ctgtaaaatt gaagataaaa acgcgatgga gcgtatttgt cgtattcgcc
    14821 agctgccgga cggtcacaac tttaccctga tgtgtcgcga tctttctgaa ctctcgacct
    14881 attcatttgt tgataacgtt gcgtttcgtt taatgaaaaa caacacgccg ggcaactata
    14941 ccttcatctt gaaggggacg aaggaagtgc cacgccgtct gttgcaggaa aaacgcaaaa
    15001 ccatcggtat gcgtgtgcct tctaacccga ttgcgcaggc gttgcttgaa gcactgggcg
    15061 aaccgatgct ttccacttcg ctaatgctgc caggcagcga atttaccgaa tccgatcccg
    15121 aagaaattaa agaccgtctg gaaaaacagg tggatttgat tatccacggc ggctatctcg
    15181 gacagaaacc gacaacggtt attgatctta ccgatgatac gccggtcgtg gtgcgtgaag
    15241 gcgtaggtga tgtgaagcct ttcttataag tgcgtaactc tgtatactac gcggccttaa
    15301 aatggcgcta tacggcgcca ttgattctcc tgttcgacgc ctgtgaaggc gacaccaaaa
    15361 ggaagctcta tgagcgaaaa attacagaaa gtgctggccc gcgctggcca cggctcccgc
    15421 cgtgaaatcg aatctattat tgaagccggt cgtgtgagtg ttgatggcaa aattgctaaa
    15481 ctcggcgatc gcgttgaagt tacccctggg ctgaaaattc gtatcgatgg tcatctgatt
    15541 tcggtacgtg agtccgctga acaaatttgt cgcgttctgg cctattacaa accggaaggt
    15601 gagttgtgta cccgtaacga tccggaaggg cgtccgacgg tgtttgaccg tctgccaaag
    15661 ctgcgtggcg cacgctggat tgccgtaggt cgtctggacg ttaacacctg tggtctgctg
    15721 ctgttcacca ccgatggtga actggcaaac cgtttaatgc atccaagccg tgaagttgaa
    15781 cgtgaatacg ccgtgcgtgt atttggtcag gttgacgacg cgaaactgcg tgatttgagt
    15841 cgtggcgtgc aactggaaga tggtccggca gcttttaaaa ccatcaagtt cagcggcggc
    15901 gaagggatca accagtggta caacgtgact ctgaccgaag gccgtaaccg tgaagttcgt
    15961 cgtctgtggg aagcggttgg tgtgcaggtg agccgcctga tccgtgttcg ttacggtgat
    16021 atcccactgc caaaaggtct gccacgcggt ggctggacgg agctggacct cgcccagact
    16081 aactatctgc gcgaactggt ggagcttccg ccagaaacca gctctaaagt cgctgtagaa
    16141 aaagaccgtc gtcgcatgaa ggctaatcag attcgtcgtg cggtgaaacg tcacagtcag
    16201 gtgagcggcg gtcgtcgttc tggcgggcgt aataacggtt aatcaaagta tgccggacgt
    16261 catatccggc atttttacag attaataatc aatccctatc tgcgctttta caccggcatc
    16321 aaacgcatgt ttgacggggc gtaattcact taccgtatct gccagttcaa gaatatcccg
    16381 atgacaacca cgacccgtga taatcaccgt ctgttgatgt ggacgttcat ttaacgcctg
    16441 caccacttct tccagtggca aatagtcata cgccaccata tacgtcagtt catcaagcaa
    16501 aaccatatcc agtgaggaat cagcaagcat ccgctttgca tgttgccaga cttcgcggca
    16561 ggcggcggta tcagactcgc ggttttgtgt atcccaggta aagcccgttg ccatcacctg
    16621 aaactcaacg ccatgtggct ccagcagatt gcgttcgcca ttaggccagg tgcctttaat
    16681 aaactgcacg acgcctactt tttttccgtg accaactgcg cgtgttgccg taccaaatgc
    16741 cgcggtggtt ttgccttttc cattgccggt aaagacgatg ataataccgc gttcatcctg
    16801 ggcctgggcc acacgagcat ctactttttc tttcactcgc tgctgacgct gttggtagcg
    16861 ttcatcactc attgggaaat tcctggttta cggcccggtt gggcgtcaaa ggtcatgccg
    16921 gttttacggc ggctgtcatc gcccatcagc cagaggtaga gcggcatgat atcagcgggt
    16981 gttttaagtt tctgtggatc ttcggtcggg aaggcgcttg cgcgcattgc ggtgcgcgta
    17041 ccgcctgggt taatgcagtt gacgcgcagg cgctgctgat attcatcggc cagtacctgc
    17101 atcatccctt cggtggcaaa tttcgacgct gcatatgcac cccagttagc tcgtccctga
    17161 cgtccaacgc ttgatgaagt aaagaccagt gaaccggcgt ccgatttgag taataaagga
    17221 agcagtgcct gggtgagcat aaaggtggca ttaacgttga cctgcatgac gtcctgccag
    17281 acctgcggat tttgttcgct cattgggcaa atatcgccga gcaatccggc attatgcaaa
    17341 acaccatcca gacgcggata attaacgaca atgcgctgtg ccagttgttg gcaattttcg
    17401 gacgtgcagg tcagcaaatc gagaataaac cactgtggct gacgcccagt ttcttcgttt
    17461 atgtggctgg ctacctgacg taatttttct tcattacggc ccaacagaat cactgtcgca
    17521 ccatagcgtg catacgtcat cgcggcttca cgaccaatcc catcgctggc tcccgtcacc
    17581 aggataatgc gatcattgag taaatcttgt tttggctggt aatgcatggc tactcctcaa
    17641 cgacgttgtc tgtcgcgctg ccagtgtact ttatgacttt ggggctttat gcctgaaaca
    17701 gaagggcatt tcaatcagcc tggggcgata acaccgcaaa atggatactt tgtcatactt
    17761 tcgctgcaat aacatctctg cgagacggct taacatgcct gttgtaaact gtgagccaaa
    17821 gcgttgttta accaaggtgg ggactcgtgg aattgttgtc tgaatatggt ttgtttttgg
    17881 cgaaaatcgt taccgttgtg ctagcgattg cggcgattgc cgccattatt gtcaatgttg
    17941 ctcaacgtaa taaacgccag cgtggcgagt tacgggtcaa caatctcagc gaacagtata
    18001 aggagatgaa agaagaactg gccgcggcgc tgatggactc acatcagcaa aaacagtggc
    18061 acaaagcgca gaagaaaaag cacaagcaag aagcgaaagc agcaaaagcg aaagccaaac
    18121 tgggcgaggt ggcaactgac agtaaacctc gcgtctgggt gctggatttt aaaggcagca
    18181 tggacgccca tgaagtgaac tcgctacgtg aagagataac ggctgtactc gcagcattca
    18241 aaccgcagga tcaggttgtg ctacgtctgg aaagccctgg tggcatggtg catggttacg
    18301 ggttggcggc ttcgcagctg cagcgtctgc gtgataaaaa cattccttta actgttacgg
    18361 tagacaaagt cgctgccagc ggcggttaca tgatggcctg tgtggcggac aaaattgttt
    18421 ccgcaccgtt tgctattgtg ggttccattg gcgtggtggc gcaaatgccc aactttaacc
    18481 gcttcctgaa aagcaaagat attgatatcg aactgcacac tgccgggcag tataagcgta
    18541 ctctgacctt gctgggtgaa aataccgaag aagggcggga gaaattccgc gaagagttga
    18601 acgaaacgca tcagttgttt aaagattttg tgaagcgtat gcgtccgtct ctggatattg
    18661 aacaggtggc aacgggtgaa cactggtacg gacaacaggc ggtagagaaa ggcctggttg
    18721 atgaaatcaa caccagtgat gaagttattc ttagcctgat ggaaggccgt gaagtggtta
    18781 atgtacgcta tatgcagcgt aaacgactca ttgaccgatt caccggcagc gcggcagaga
    18841 gcgccgatcg attgttgcta cgctggtggc agcggggtca aaagccattg atgtaaaaga
    18901 caaacgcgag gctaagacct cgcgttttgc tttaatcaac cagatgatat ttttctgaaa
    18961 gcacatgggc caggtgtttg aacatattaa acaccgcggt gcttttggct gttggcaatc
    19021 cttgttcatc taaaaagtag tcgccggtaa ataccagcac gccattacgc tgcatgacgc
    19081 cggtggcttc aatccctgcg agcgtatcct catgctcacg aatgattttg ttggcctctt
    19141 tcaacagcgt ttcgcggtcg atgggttgtg tctctttgtt cattatttac tccttaaaca
    19201 aggacattag tctacgccag gcatggcttg cagacaaata taccacgctg gtggcaagag
    19261 cgccttactg gtaactttgg attttgcatg ctaataaagt tgcgtatcgg attttatcag
    19321 gtacagtgtg acgctttcgt caatctggca atagatttgc ttgacattcg accaaaattc
    19381 cgtcgtgcta tagcgcctgt aggccaagac ctgttaactc agtcacctga attttcgtga
    19441 acagagtcac gacaaggggt tgatatccgc agagaacgag tccatatcgg taactcgttg
    19501 ccagtggaag gtttatcaac gtgcgacgca ttcctggaag aatcaaatta ggtaaggtga
    19561 atatgggtaa agctcttgtc atcgttgagt ccccggcaaa agccaaaacg atcaacaagt
    19621 atctgggtag tgactacgtg gtgaaatcca gcgtcggtca catccgcgat ttgccgacca
    19681 gtggctcagc tgccaaaaag agtgccgact ctacctccac caagacggct aaaaagccta
    19741 aaaaggatga acgtggcgct ctcgtcaacc gtatgggggt tgacccgtgg cacaattggg
    19801 aggcgcacta tgaagtgttg cctggtaaag agaaggtcgt ctctgaactg aaacaactgg
    19861 ctgaaaaagc cgaccacatc tatctcgcaa ccgaccttga ccgcgaaggg gaagccattg
    19921 catggcacct gcgggaagtg attgggggtg atgatgcgcg ctatagccga gtggtgttta
    19981 acgaaattac taaaaacgcg atccgccagg catttaacaa accgggtgag ctgaatattg
    20041 atcgtgttaa tgcccagcag gcgcgtcgct ttatggaccg cgtggttggg tatatggttt
    20101 cgccgctgct atggaaaaag atcgctcgtg gtctgtctgc cggtcgtgtg cagtcggtgg
    20161 cggttcgcct ggtggtcgag cgtgagcgtg aaattaaagc gttcgtgccg gaagagttct
    20221 gggaagtcga tgccagcacg accacgccat ctggtgaagc gttggcgtta caggtgactc
    20281 atcagaacga caaaccgttc cgtccggtca acaaagaaca aactcaggct gcggtaagtc
    20341 tgctggaaaa agtgcgctac agcgtgctgg aacgtgaaga caaaccgaca accagtaaac
    20401 ctggcgctcc ttttattacc tctacgctgc aacaagctgc cagcacccgt cttggatttg
    20461 gcgtgaaaaa aaccatgatg atggcgcagc gtttgtatga agcaggctat atcacttaca
    20521 tgcgtaccga ctccactaac ctgagtcagg acgcggtaaa tatggttcgc ggttatatca
    20581 gcgataattt tggtaagaaa tatctgccgg aaagtccgaa tcagtacgcc agcaaagaaa
    20641 actcacagga agcgcacgaa gcgattcgtc cttctgacgt caatgtgatg gcggaatcgc
    20701 tgaaggatat ggaagcagat gcgcagaaac tgtaccagtt aatctggcgt cagttcgtag
    20761 cctgccagat gaccccagcg aaatatgact ccacgacgct gaccgttggt gcgggcgatt
    20821 tccgcctgaa agcacgcggt cgtattttgc gttttgatgg ctggacaaaa gtgatgcctg
    20881 cgttgcgtaa aggcgatgaa gatcgcatct taccagcagt taataaaggc gatgctctga
    20941 cgctcgttga acttacacca gcccagcact ttaccaagcc gccagcccgt ttcagtgaag
    21001 catcgctggt taaagagctg gaaaaacgtg gtatcggtcg tccgtctacc tatgcgtcga
    21061 tcatttcgac cattcaggat cgtggctacg tgcgagtaga aaatcgtcgt ttctatgcgg
    21121 aaaaaatggg cgaaatcgtc accgatcgcc ttgaagaaaa tttccgcgag ttaatgaact
    21181 acgactttac cgcgcagatg gaaaacagcc tcgaccaggt ggcaaatcac gaagcagagt
    21241 ggaaagctgt actggatcac ttcttctcgg atttcaccca gcagttagat aaagctgaaa
    21301 aagatccgga agagggtggt atgcgcccga accagatggt tctgaccagc attgactgcc
    21361 cgacttgtgg tcgcaaaatg gggattcgca cagcgagcac cggggtattc cttggctgtt
    21421 ctggctatgc gctgccgccg aaagagcgtt gcaaaaccac cattaacctg gtgccggaaa
    21481 acgaagtgct gaacgtactg gaaggcgaag acgctgaaac caacgcgctg cgcgcaaaac
    21541 gtcgttgccc gaaatgcggc acggcgatgg acagctatct catcgatccg aaacgtaagt
    21601 tgcatgtctg tggtaataac ccaacctgcg acggttacga gatcgaagag ggcgaattcc
    21661 gcattaaagg ttatgacggc ccgatcgttg agtgtgaaaa atgtggctct gaaatgcacc
    21721 tgaaaatggg gcgtttcggt aaatacatgg cctgcaccaa cgaagagtgt aaaaacacac
    21781 gtaagatttt acgtaacggc gaagtggcac caccgaaaga agatccggtg ccattacctg
    21841 agctgccgtg cgaaaaatca gatgcttatt tcgtgctgcg tgacggtgct gccggtgtgt
    21901 tcctggctgc caacactttc ccgaaatcgc gtgaaacgcg tgcgccactg gtggaagagc
    21961 tttatcgctt ccgcgaccgt ctgccggaaa aactgcgtta tctggccgat gcgccacagc
    22021 aggatccgga aggtaataag accatggttc gctttagccg taaaaccaaa cagcaatatg
    22081 tctcttcgga aaaagacgga aaggcgactg gctggtcagc attttatgtt gatggcaaat
    22141 gggttgaagg aaaaaaataa cctttaattc tgtcaggttt ttataaacaa agggtcgcga
    22201 aagcggccct tttttattgc atattattt
//
LOCUS       DAZFQD010000081        21751 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.081, whole genome shotgun sequence.
ACCESSION   DAZFQD010000081 DAZFQD010000000
VERSION     DAZFQD010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21751
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.081"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(95..826)
                     /gene="traT"
                     /locus_tag="ACHKTW_003094"
     CDS             complement(95..826)
                     /gene="traT"
                     /locus_tag="ACHKTW_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer complement resistance protein
                     TraT"
                     /protein_id="HFO6464068.1"
                     /translation="MKTKKLMMVALVSSTLALSGCGAMSTAIKKRNLEVKTQMSETIW
                     LEPASERTVFLQIKNTSDKDMSGLQGKIADAVKAKGYQVVTSPDKAYYWIQANVLKAD
                     KMDLRESQGWLNRGYEGAAVGAALGAGITGYNSNSAGATLGVGLAAGLVGMAADAMVE
                     DVNYTMITDVQIAERTKATVTTDNVAALRQGTSGAKIQTSTETGNQHKYQTRVVSNAN
                     KVNLKFEEAKPVLEDQLAKSIANIL"
     gene            complement(851..1372)
                     /gene="traS"
                     /locus_tag="ACHKTW_003095"
     CDS             complement(851..1372)
                     /gene="traS"
                     /locus_tag="ACHKTW_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000632670.1"
                     /note="TraS, as the term is used in F-like plasmids, is a
                     plasmid entry exclusion protein. It interacts with the
                     F-like plasmid mating stabilization protein TraG; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer entry exclusion protein TraS"
                     /protein_id="HFO6464069.1"
                     /translation="MITQQIISSELEVLKKHIDSGDIRIPSLWQGLKPGLIIMGWMIF
                     CPLLMSFLITQKTSETLTAVLAGGWLGLIILFIVARIRMLYFSLPEEFLKTSSVMRVI
                     SSKLKVYFIVYMGVIFLWSFLGGGIIYGFGAILVTVIMAFLIQLDIGRYQFVGVIDAI
                     NSYVKNKKLSRVK"
     gene            complement(1405..4221)
                     /gene="traG"
                     /locus_tag="ACHKTW_003096"
     CDS             complement(1405..4221)
                     /gene="traG"
                     /locus_tag="ACHKTW_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001733890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer mating-pair stabilization
                     protein TraG"
                     /protein_id="HFO6464070.1"
                     /translation="MNEVYVIAGGEWLRNNLNAIAAFMGTWTWDSIEKIALTLSVLAV
                     AVMWVQRHNVMDLLGWVAVFVLISLLVNVRTSVQIIDNSDLVKVHRVDNVPVGLAMPL
                     SLTTRIGHAMVASYEMIFTQPDSVTYSKTGMLFGANLIVKSTDFLSRNPEIINLFQDY
                     VQNCVLGDIYLNHKYTLEDLMASADPYTLIFSRPSPLRGVYDNNNNFITCKDASVTLK
                     DRLNLDTKTGGKTWHYYVQQIFGGRPDPDLLFRQLVSDSYSYFYGSSQSASQIMRQNV
                     TMNALKEGITSNAARNGDTASLVSLATTSSMEKQRLAHVSIGHVTMRNLPMVQTILTG
                     IAIGIFPLLILAAVFNKLTLSVLKGYVFALMWLQTWPLLYAILNSAMTFYAKQNGAPV
                     VLSELSQIQLKYSDLASTAGYLSAMIPPLSWMMVKGLGAGFSSVYSHFASSSISPTAS
                     AAGSVVDGNYSYGNMQTENVNGFSWSTNSTTSFGQMMYQTGSGATATQTRDGNMVMDA
                     SGAMSRLPVGINATRQIAAAQQEMAREASNRAESALHGFSSSIASAWNTLSQFGSNRG
                     SSDSVTGGADSTMSAQDSMMASRMRSAVESYAKAHNISNEQATRELASRSTNASLGLY
                     GDAYAKGHLGISVLGNGGGVGLQAGAKASIDGSDLDSHEASSGSRASHDARHDIDARA
                     TQDFKEASDYFTSRKVSESGSHTDNNADSRVDQLSAALNSAKQSYDQYTTNMTRSHEY
                     AEMASRTESMSGQMSEDLSQQFAQYVMKNAPQDVEAILTNTSSPEIAERRRAMAWSFV
                     QEQVQPGVDNTWRESRRDIGKGMESVPSGGGSQDIIADHQGHQAIIEQRTQDSNIRND
                     VKHQVDNMVTEYRGNIGDTQNSIRGEENIVKGQYSELQNHHKTEALTQNNKYNEEKLA
                     QERIPGADSPKELLEKAKSYQHKK"
     gene            complement(4218..5591)
                     /gene="traH"
                     /locus_tag="ACHKTW_003097"
     CDS             complement(4218..5591)
                     /gene="traH"
                     /locus_tag="ACHKTW_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus assembly protein TraH"
                     /protein_id="HFO6464071.1"
                     /translation="MLRIKPLFILCAALLTVTPAASADVNSDMNQFFNKLGFASNTTQ
                     PGVWQGQAAGYAYGGSLYARTQVKNVQLISMTLPDINAGCGGIDAYLGSFSFINGEQL
                     QRFVKQIMSNAAGYFFDLALQTTVPEIKTAKDFLQKMASDINSMNLSSCQAAQGIIGG
                     LFPRTQVSQQKVCQDIAGESNIFADWAASRQGCTVGGKSDSVRDKASDKDKERVTKNI
                     NIMWNALSKNRMFDGNKELKEFVMTLTGSLVFGPNGEITPLSARTTDRSIIRAMMEGG
                     TAKIYHCNDSDKCLKVVADTPVTISRDNALKSQITKLLASIQNKAVSDTPLDDKEKGF
                     ISSTTIPVFKYLVDPQMLGVSNSMIYQLTDYIGYDILLQYIQELIQQARAMVATGNYD
                     EAVIGHINDNMNDATRQIAAFQSQVQVQQDALLVVDRQMSYMRQQLSARMLSRYQNNY
                     HFGGSTL"
     gene            complement(5578..5970)
                     /gene="trbF"
                     /locus_tag="ACHKTW_003098"
     CDS             complement(5578..5970)
                     /gene="trbF"
                     /locus_tag="ACHKTW_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001700179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F-type conjugal transfer protein TrbF"
                     /protein_id="HFO6464072.1"
                     /translation="MRENKNNPELKIRSTERDYKYISRITDRYAGLPLVFLTAGIVLW
                     TAMNIIFDACIDSWKMDQELNNSIYMWNILIYVIPYALYALAVGFLVTFFLFPMYELI
                     YGNIGIFLLKRRMRRENTLREGSNNASH"
     gene            complement(5957..6298)
                     /gene="trbJ"
                     /locus_tag="ACHKTW_003099"
     CDS             complement(5957..6298)
                     /gene="trbJ"
                     /locus_tag="ACHKTW_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein TrbJ"
                     /protein_id="HFO6464073.1"
                     /translation="MPPVLWRGWIPFCRCTEGKKVRNKQVVLLIAGISGIATGIIVSL
                     NIPFIHQGLFYPASPVEIVVSLCLTFSVSVVFFVGAIVGWISVSEIYYSRRTGLNELS
                     EISEGTYNERK"
     gene            complement(6228..6773)
                     /gene="trbB"
                     /locus_tag="ACHKTW_003100"
     CDS             complement(6228..6773)
                     /gene="trbB"
                     /locus_tag="ACHKTW_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000052622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly thiol-disulfide isomerase TrbB"
                     /protein_id="HFO6464074.1"
                     /translation="MSLTKSLLFTLLLSAAAVQASTRDEIERLWNPQGMATQPAQPAA
                     GTSARTAKPAPRWFRLSNGRQVNLADWKVVLFMQGHCPYCHQFDPVLKQLAQQYGFSV
                     FPYTLDGQGDTAFPEALPVPPDVMQTFFPNIPVATPTTFLVNVNTLEALPLLQGATDA
                     AGFMARMDTVLQMYGGKKGAK"
     gene            complement(6760..7044)
                     /gene="traQ"
                     /locus_tag="ACHKTW_003101"
     CDS             complement(6760..7044)
                     /gene="traQ"
                     /locus_tag="ACHKTW_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001617873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     chaperone TraQ"
                     /protein_id="HFO6464075.1"
                     /translation="MISKRRFSLPRLDITGMWVFSLGVWFHIVARLVYSKPWMAFFLA
                     ELIAAILVLFGAYQVLDAWIARVSREEREALEARQQAMMEGQQEGGHVSH"
     gene            7125..7439
                     /locus_tag="ACHKTW_003102"
     CDS             7125..7439
                     /locus_tag="ACHKTW_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001490797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin ArtA"
                     /protein_id="HFO6464076.1"
                     /translation="MEKRSFKEKLEIIRNIIRESLLGNAAIIALIYAASHSLPVNAFP
                     DYLVISLLSIAAVIVVLWLFSIIYIYFCELFRSHWIAVWFIIWSSVINLIILYGFYDH
                     FI"
     gene            complement(7441..7788)
                     /gene="trbA"
                     /locus_tag="ACHKTW_003103"
     CDS             complement(7441..7788)
                     /gene="trbA"
                     /locus_tag="ACHKTW_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbA"
                     /protein_id="HFO6464077.1"
                     /translation="MSEDYLKMFTGVVLLMFVIIAGYFFSERNDRKMFLLSSLVFLVI
                     NIACLYVLTASLWFLCGAIMSQGAALVVSIVAAALPDVTSFDRFRRIFICIVLSSVWS
                     GVMWFFIRGLMTG"
     gene            complement(7804..8547)
                     /gene="traF"
                     /locus_tag="ACHKTW_003104"
     CDS             complement(7804..8547)
                     /gene="traF"
                     /locus_tag="ACHKTW_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TraF"
                     /protein_id="HFO6464078.1"
                     /translation="MNKALLPLLLCCFIFPASGKDAGWQWYNEKINPKEKENKPVPAA
                     PRQEPDIMQKLAALQTATKRALYEAILYPGVDNFVKYFRLQNYWTQQAGLFTMSARKA
                     MLAHPELDYNLQYSHYNGTVRNQLAADQAQQRQAIAKLAEHYGIMFFYRGQDPIDGQL
                     AQVINGFRDTYGLSVIPVSVDGVINPLLPDSRTDQGQAQRLGVKYFPAMMLVDPKQGS
                     VRPLSYGFISQDDLAKQFLNVSEDFKPNF"
     gene            complement(8540..8797)
                     /locus_tag="ACHKTW_003105"
     CDS             complement(8540..8797)
                     /locus_tag="ACHKTW_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbE"
                     /protein_id="HFO6464079.1"
                     /translation="MKVIFTSNRFIDFLIRLLITAIVISPVIIWSWDTVKETTADGML
                     AAAFVILYSGVLLFILYFCFSALTDLQKPDERKSDERNEDE"
     gene            complement(8824..10632)
                     /gene="traN"
                     /locus_tag="ACHKTW_003106"
     CDS             complement(8824..10632)
                     /gene="traN"
                     /locus_tag="ACHKTW_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001756807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system mating-pair
                     stabilization protein TraN"
                     /protein_id="HFO6464080.1"
                     /translation="MKRILPLILALVAGMAQADSNSDYRAGSDFAHQIKGQGSSSIQG
                     FKPQESIPGYNANPDETKYYGGVTAGGDGGLKNDGTTEWATGETGKTITESFMNKPKD
                     ILSPDAPFIQTGRDVVNRADSIVGNTGQQCSAQEISRSEYTNYTCERDLQVEQYCTRT
                     ARMELQGSTTWETRTLEYEMSQLPAREVNGQYVVSITSPVTGEIVDAHYSWSRTYLQK
                     SVPMTITVLGTPLSWNAKYSADASFTPVQKTLTAGVAFTSSHPVRVGNTKFKRHTAMK
                     LRLVVRVKKASYTPYVVWSESCPFSKELGKLTKTECTEAGGNRTLVKDGQSYSMYQSC
                     WAYRDTYVTQSADKGTCQTYTDNPACTLVSHQCAFYSEEGACLHEYATYSCESKTSGK
                     VMVCGGDVFCLDGECDKAQSGQSNDFAEAVSQLAALAAAGKDVAALNGVDVRAFTGQA
                     KFCKKAAAGYSNCCKDSGWGQDIGLAKCSSDEKALAKAKSNKLTVSVGEFCSKKVLGV
                     CLEKKRSYCQFDSKLAQIVQQQGRNGQLRISFGSAKHPDCRGITVDELQKIQFNRLDF
                     TNFYEDLMNNQKIPDSGVLTQKVKEQIADQLKQAGQ"
     gene            complement(10629..11267)
                     /gene="trbC"
                     /locus_tag="ACHKTW_003107"
     CDS             complement(10629..11267)
                     /gene="trbC"
                     /locus_tag="ACHKTW_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001553832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TrbC"
                     /protein_id="HFO6464081.1"
                     /translation="MKLSMKSLAALLMMLNGAVMASENVNTPENRQFLKQQENLSRQL
                     REKPDHQLKAWAEKQVLENPLQRSDNHFLDELVRKQQASQDGKPRQGALYFVSFSIPE
                     EGLKRMLGETRHYGIPATLRGMVNNDLKTTAEAVLSLVKDGATDGVQIDPTLFSQYGI
                     RTVPALVVFCSQGYDIIRGNLRVGQALEKVAATGDCRQVAHDLLAGKGDSGK"
     gene            complement(11276..12268)
                     /gene="traU"
                     /locus_tag="ACHKTW_003108"
     CDS             complement(11276..12268)
                     /gene="traU"
                     /locus_tag="ACHKTW_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus assembly protein TraU"
                     /protein_id="HFO6464082.1"
                     /translation="MKRSLWLLMLFLLAGHVPAASADSACEGRFVNPITDICWSCIFP
                     LSLGSIKVSQGKVPDTANPSMPIQICPAPPPLFRRIGLAIGYWEPMALTDVTRSPGCM
                     VNLGFSLPAFGKTAQGTAKKDEKQVNGAFYHVHWYKYPLTYWLNIITSLGCLEGGDLD
                     IAYLSEIDPTWTDSSLTTILNPEAVIFANPIAQGACAADAIASAFNMPLDVLFWCAGS
                     QGSMYPFNGWVSNESSPLQSSLLVSERMAFKLHRQGMIMETIGKNNAVCNEYPSPILP
                     KERWRYQMVNMYPDSGQCHPFGRSVTRWETGKNPPNTKKNFGYLMWRKRNCVFL"
     gene            complement(12265..12897)
                     /gene="traW"
                     /locus_tag="ACHKTW_003109"
     CDS             complement(12265..12897)
                     /gene="traW"
                     /locus_tag="ACHKTW_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TraW"
                     /protein_id="HFO6464083.1"
                     /translation="MRCRGLIALLIWGQSVAAADLGTWGDLWPVKEPDMLTVIMQRLT
                     ALEQSGEMGRKMDAFKERVIRNSLRPPAVPGIGRTEKYGSRLFDPSVRLAADIRDNEG
                     RVFARQGEVMNPLQYVPFNQTLYFINGDDPAQVAWMKRQTPPILESKIILVQGSIPEM
                     QKSLDSRVYFDQNGVLCQRLGIDQVPARVSAVPGDRFLKVEFIPAEEGRK"
     gene            complement(12894..13280)
                     /gene="trbI"
                     /locus_tag="ACHKTW_003110"
     CDS             complement(12894..13280)
                     /gene="trbI"
                     /locus_tag="ACHKTW_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TrbI"
                     /protein_id="HFO6464084.1"
                     /translation="MSSTQKPADVTAERRSHWWWTVPGCLAMVLLNAAVSYGIVRLNA
                     PVTVAFNMKQTVDAFFDSASQKQLSEAQSKALSARFNTALEASLQAWQQKHHAVILVS
                     PAVVQGAPDITREIQQDIARRMRAEP"
     gene            complement(13277..15904)
                     /gene="traC"
                     /locus_tag="ACHKTW_003111"
     CDS             complement(13277..15904)
                     /gene="traC"
                     /locus_tag="ACHKTW_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001064255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein TraC"
                     /protein_id="HFO6464085.1"
                     /translation="MNNPLEAVTQAVNSLVTALKLPDESAKANEVLGEMSFPQFSRLL
                     PYRDYNQESGLFMNDTTMGFMLEAIPINGANESIVEALDHMLRTKLPRGIPLCIHLMS
                     SQLVGDRIEYGLREFSWSGEQAERFNAITRAYYMKAAATQFPLPEGMNLPLTLRHYRV
                     FISYCSPSKKKSRADILEMENLVKIIRASLQGASITTQTVDAQAFIDIVGEMINHNPD
                     SLYPKRRQLDPYSDLNYQCVEDSFDLKVRADYLTLGLRENGRNSTARILNFHLARNPE
                     IAFLWNMADNYSNLLNPELSISCPFILTLTLVVEDQVKTHSEANLKYMDLEKKSKTSY
                     AKWFPSVEKEAKEWGELRQRLGSGQSSVVSYFLNITAFCKDNNETALEVEQDILNSFR
                     KNGFELISPRFNHMRNFLTCLPFMAGKGLFKQLKEAGVVQRAESFNVANLMPLVADNP
                     LTPAGLLAPTYRNQLAFIDIFFRGMNNTNYNMAVCGTSGAGKTGLIQPLIRSVLDSGG
                     FAVVFDMGDGYKSLCENMGGVYLDGETLRFNPFANITDIDQSAERVRDQLSVMASPNG
                     NLDEVHEGLLLQAVRASWLAKENRARIDDVVDFLKNASDSEQYAESPTIRSRLDEMIV
                     LLDQYTANGTYGQYFNSDEPSLRDDAKMVVLELGGLEDRPSLLVAVMFSLIIYIENRM
                     YRTPRNLKKLNVIDEGWRLLDFKNHKVGEFIEKGYRTARRHTGAYITITQNIVDFDSD
                     KASSAARAAWGNSSYKIILKQSAKEFAKYNQLYPDQFLPLQRDMIGKFGAAKDQWFSS
                     FLLQVENHSSWHRLFVDPLSRAMYSSDGPDFEFVQQKRKEGLSIHEAVWQLAWKKSGP
                     EMASLEAWLEEHEKYRSVA"
     gene            complement(16064..16285)
                     /gene="traR"
                     /locus_tag="ACHKTW_003112"
     CDS             complement(16064..16285)
                     /gene="traR"
                     /locus_tag="ACHKTW_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraR"
                     /protein_id="HFO6464086.1"
                     /translation="MSDEADEAYSVTEQLTMTGINRIRQKINAHGIPVYLCEACGNPI
                     PEARRKIFPGVTLCVECQAYQERQRKHYA"
     gene            complement(16420..16935)
                     /gene="traV"
                     /locus_tag="ACHKTW_003113"
     CDS             complement(16420..16935)
                     /gene="traV"
                     /locus_tag="ACHKTW_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system lipoprotein
                     TraV"
                     /protein_id="HFO6464087.1"
                     /translation="MKQISFFIPLLGTLLLSGCAGTSTEFECNATTSDTCMTMEQANE
                     KAKKLEQSSEAKPVAASLPRLAEGNFRTMPVQTVTATTPSGSRPAVTAHPEQKLLAPR
                     PLFTAAREVKTVVPVSSVTPVTPPRPLRTGEQTAALWIAPYIDNQDVYHQPSSVFFVI
                     KPSAWGKPRIN"
     gene            complement(16932..17183)
                     /gene="trbG"
                     /locus_tag="ACHKTW_003114"
     CDS             complement(16932..17183)
                     /gene="trbG"
                     /locus_tag="ACHKTW_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001038337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbG"
                     /protein_id="HFO6464088.1"
                     /translation="MNKLVSDGSVKKINYPVLYESGITPPLCEVSAPEPDAGGKRIVA
                     YVYKSSRSTVFENPDIVKTCTVRDLKKDFVNCDEKGEGQ"
     gene            complement(17176..17496)
                     /locus_tag="ACHKTW_003115"
     CDS             complement(17176..17496)
                     /locus_tag="ACHKTW_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001377845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbD"
                     /protein_id="HFO6464089.1"
                     /translation="MNMRNINVITALSVPGKTVSDDFMHAVLSNCATRIVLPAPEKFG
                     SESLPDNFNMAAVGVMKKGMSMSLFSSPGHIVVAGQSGGGKSVPVKELIDRIIQKYKE
                     PFDE"
     gene            complement(17483..18073)
                     /gene="traP"
                     /locus_tag="ACHKTW_003116"
     CDS             complement(17483..18073)
                     /gene="traP"
                     /locus_tag="ACHKTW_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001528624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus-stabilizing protein
                     TraP"
                     /protein_id="HFO6464090.1"
                     /translation="MANNMSSRQACHAARYVVARVLRGLFWCLKYTVILPLATMALMA
                     LFVLWKDNTTPGKLLVKEINFVRQTAPAGQFPVSECWFSSSDSSGRSEIQDICHYRAA
                     DAADYVRETDRSLMQLVTALWATLALMYVSLAAITGKYPVRPGKMKCIRVVTADEHLK
                     EVYTEDASLPGKIRKCPVYLPDDRTNRNNGDKNEHA"
     gene            complement(18063..19490)
                     /gene="traB"
                     /locus_tag="ACHKTW_003117"
     CDS             complement(18063..19490)
                     /gene="traB"
                     /locus_tag="ACHKTW_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001553749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F-type conjugal transfer pilus assembly protein
                     TraB"
                     /protein_id="HFO6464091.1"
                     /translation="MASINTIVKRKQYLWLGIVVVGTASAIGGALYLSDVDMSGNGET
                     VAEQEPVPDMTGVVDTTFDDKVRQHATTEMQVTAAQMQKQYEEIRRELDVLNKQRGDD
                     QRRIEKLGQDNAALAEQVKALGANPVTATGEPVPQMPASPPGPEGEPQPGNTPVSFPP
                     QGSVAVPPPTAFYPGNGVTPPPQVTYQSVPVPNRIQRKVFTRNEGKQGPSLPYIPSGS
                     FAKAMLIEGADANASVTGNESTVPMQLRITGLVEMPNSKTYDATGCFVGLEAWGDVSS
                     ERAIVRTRNISCLKDGKTIDMPIKGHVSFRGKNGIKGEVVMRNGKILGWAWGAGFVDG
                     IGQGMERASQPAVGLGATAAYGAGDVLKMGIGGGASKAAQTLSDYYIKRAEQYHPVIP
                     IGAGNEVTVVFQDGFQLKTVEEMALERTQSRAEEDNPESPVPVPPSAESHLNGFNTDQ
                     MLKQLGNLNPQQFMSGSQGGGNDGK"
     gene            complement(19490..20218)
                     /gene="traK"
                     /locus_tag="ACHKTW_003118"
     CDS             complement(19490..20218)
                     /gene="traK"
                     /locus_tag="ACHKTW_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001230803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system secretin
                     TraK"
                     /protein_id="HFO6464092.1"
                     /translation="MRKNNTAIIFGSLFFSCSVMAANGTLAPTVVPMVNGGQASIAIS
                     NTSPNLFTVPGDRIIAVNSLDGALTNNEQTASGGVVVATVNKKPFTFILETERGLNLS
                     IQAVPREGAGRTIQLVSDLRGTGEEAGAWETSTPYESLLVTISQAVRGGKLPAGWYQV
                     PVTKETLQAPAGLSSVADAVWTGNHLKMVRFAVENKTLSALNIRESDFWQPGTRAVMF
                     SQPASQLLAGARMDVYVIRDGEGN"
     gene            complement(20205..20771)
                     /gene="traE"
                     /locus_tag="ACHKTW_003119"
                     /pseudo
     CDS             complement(20205..20771)
                     /gene="traE"
                     /locus_tag="ACHKTW_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490565.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
     gene            complement(20793..21104)
                     /gene="traL"
                     /locus_tag="ACHKTW_003120"
     CDS             complement(20793..21104)
                     /gene="traL"
                     /locus_tag="ACHKTW_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraL"
                     /protein_id="HFO6464093.1"
                     /translation="MSGDENKLKKYRFPETLTNQSRWFGLPLDELIPAAICIGWGITT
                     SKYLFGIGAAVLVYFGIKKLKKGRGSSWLRDLIYWYMPTALLRGIFHNVPDSCFRQWI
                     K"
     gene            complement(21119..21484)
                     /gene="traA"
                     /locus_tag="ACHKTW_003121"
     CDS             complement(21119..21484)
                     /gene="traA"
                     /locus_tag="ACHKTW_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system pilin TraA"
                     /protein_id="HFO6464094.1"
                     /translation="MDAVLSVQGVSAPVKKKSFFSKFTRLNMLRLARAVIPAAVLMMF
                     FPQLAMAAGSSGQDLMASGNTTVKATFGKDSSVVKWVVLAEVLVGAVMYMMTKNVKFL
                     AGFAIISVFIAVGMAVVGL"
     gene            complement(21527..21742)
                     /gene="traY"
                     /locus_tag="ACHKTW_003122"
     CDS             complement(21527..21742)
                     /gene="traY"
                     /locus_tag="ACHKTW_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000086381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer relaxosome protein TraY"
                     /protein_id="HFO6464095.1"
                     /translation="MSRGRTRAAPGNKVLLILDETTNQKLLAARDRSGRTKTNEVFIR
                     LKDHLNRFPDFYNASLVKEEAESIDDI"
BASE COUNT         5135 a         5985 c         5126 g         5505 t
ORIGIN      
        1 gaaataaaat ttattttatt tctttccggg gttggacaac gagcaaagcg aggcgtcagt
       61 caggaggccg gtcagaccag cctccggaag ataatcagag aatatttgcg attgatttgg
      121 ccagctggtc ttcgagaaca ggcttcgcct cttcaaattt caggttaacc ttgttcgcat
      181 ttgagaccac acgggtctgg tatttatgct ggttacctgt ttctgtactg gtctgaattt
      241 tcgcacctga tgtgccctga cgcagggcgg caacattatc cgttgtcacc gttgccttag
      301 tacgctctgc aatctgcaca tccgtgatca tggtatagtt cacatcttcc accatcgcat
      361 ctgcagccat acccaccaga ccggcagcaa ggcccacacc gagtgtggca ccggcagaat
      421 tggaattata tccggtgata ccggcaccta acgctgcacc aactgctgcg ccttcataac
      481 cacggttcag ccatccctga gactcccgca gatccatctt atcggccttc agcacattcg
      541 cctgaatcca gtagtaggct ttatccggag aagtcaccac ctgataccct tttgctttca
      601 cagcatcagc aattttgccc tgcagcccac tcatgtcttt atcagacgtg tttttgatct
      661 gcagaaatac cgtgcgttcg ctggcgggtt caagccagat ggtctcactc atctgagtct
      721 tcacctcaag gttacgcttc ttgattgctg tgctcatcgc accacaccct gaaagggcca
      781 gagtggaact gaccagtgca accatcatta gtttttttgt tttcatcata tatttactct
      841 ctaatatctt ttattttact cttgataact tcttgttttt gacgtaacta ttgattgcat
      901 caataacacc aacaaattga tatctaccga tatctaattg aatcaagaac gccataataa
      961 ccgtcactaa aattgcgcca aaaccgtata taatccctcc acctaaaaaa gaccataaaa
     1021 atataacccc catatacaca atgaaataca ctttaagttt tgatgatata accctcatta
     1081 cggatgatgt ctttaggaat tcttccggca gagagaaata taacattcta atccttgcca
     1141 caataaacag aataataagt ccaagccatc ctccggctaa cacagcagtg agtgtttcac
     1201 ttgtcttttg tgttattaga aatgacatta gcaagggaca aaaaatcatc caccccatga
     1261 tgataagccc gggttttaat ccctgccata gtgacggaat tctaatatct cctgagtcaa
     1321 tatgtttctt taaaacttcc aactcactac tgataatttg ttgtgtaatc atggtctctc
     1381 cataccctac ccaacatgtt atgattattt tttatgctgg taactctttg ctttctctaa
     1441 aagctctttc ggactatcag caccaggtat tctttcctga gccagctttt cttcattata
     1501 tttattattc tgagttaatg cttctgtttt atgatgattt tgtagttcag aatactgccc
     1561 ttttacaata ttttcctcgc ctcggatgct gttctgagta tctccgatat tacctcgata
     1621 ttctgtgacc atattatcaa cctgatgttt tacatcatta cggatattac tgtcctgcgt
     1681 tctttgctca ataatggcct gatgtccctg atgatcagca ataatatcct ggctgccacc
     1741 acccgaaggt acgctctcca ttcctttacc tatatctcta cgggattcac gccaggtgtt
     1801 atcaacacca ggctgtacct gttcctgcac aaaagaccac gccatagccc gacggcgttc
     1861 tgcaatctcc ggcgaactgg tatttgtcag aatagcttca acatcctgcg gtgcgttttt
     1921 catcacatac tgtgcaaact gttgcgacag atcttcactc atctgcccac tcatactctc
     1981 agtacgggat gccatttcag catattcatg gctgcgggtc atattcgtcg tgtactggtc
     2041 gtaactctgt ttcgctgaat tcagggctgc agacaactga tccacacggg aatcagcatt
     2101 attgtcagta tggctgccgg attcactgac tttgcggctg gtaaagtaat cgctggcttc
     2161 tttaaagtct tgtgtagctc tggcatcgat atcatgacga gcatcatggc tggcccgtga
     2221 accactactg gcttcatgtg agtcaagatc actgccatca atgcttgcct tagctccggc
     2281 ctggagacca acccctccac cgttacccaa tacacttatg cctaaatgcc ctttagcata
     2341 agcatcaccg taaaggccaa gcgacgcatt tgtacttctc gaggccagtt ctctcgtcgc
     2401 ctgctcatta ctgatattat gcgccttcgc atagctttcc actgcactgc gcatacggct
     2461 ggccatcatg gagtcctgag cactcatcgt gctgtcagca ccaccagtga cagagtcact
     2521 gctcccccgg ttagagccaa actgactgag tgtgttccag gcactggcaa tactgctgct
     2581 gaaaccatgc agggcacttt cggctctgtt cgacgcctcc cgggccattt cctgttgtgc
     2641 cgctgcaatc tgacgcgttg cattgatacc gaccggtaac cgggacatcg ctccgcttgc
     2701 atccatcacc atattaccgt cacgggtctg tgtggcggtt gcgccactgc cggtctggta
     2761 catcatctga ccaaacgacg tggtgctgtt ggtgctccag ctgaagccgt tcacgttttc
     2821 cgtctgcatg ttgccgtagg agtaattacc gtcaaccaca ctgcctgctg cactggccgt
     2881 tggactgata gatgaagagg cgaagtggct gtacacgctg gaaaaacctg cgccaaggcc
     2941 ttttaccatc atccatgaca acggggggat catggctgaa agatacccgg cagtggaggc
     3001 caggtcagag tatttcagct gtatctgaga gagttcagac aacacgaccg gcgcaccatt
     3061 ctgtttcgca tagaatgtca tggcactgtt cagaatggca tacaacaacg gccaggtctg
     3121 cagccacatc agggcaaaca catacccctt taatacagac aatgtcagct tgttaaagac
     3181 agccgccagt atcaggagcg ggaatatacc aatggcaatc cccgtcagga tagtctggac
     3241 catcggcagg tttcgcatag ttacatgacc aatggagaca tgtgccagac gttgtttctc
     3301 catcgatgac gtggtggcca gacttaccag gctggcggtg tcaccgttac gggctgcatt
     3361 actggtgata ccctctttca gggcattcat ggtgacgttc tggcgcataa tctggctggc
     3421 agactgactg gagccgtaga aataactgta actgtcactg accagttgtc tgaataacag
     3481 gtccgggtcc ggtctgccgc caaatatctg ctgcacataa taatgccagg tcttgcctcc
     3541 cgtctttgta tcgagattca gcctgtcttt cagcgtgacc gacgcatcct tacaagttat
     3601 gaaattatta ttgttgtcat aaacgccccg tagcgggctg gggcgggaaa aaatcagcgt
     3661 gtaggggtca gcggaggcca tcaggtcttc cagcgtgtat ttatgattca ggtaaatatc
     3721 acccagcacg cagttctgga cgtagtcctg gaacagattg atgatttccg gattccggga
     3781 caggaaatcg gtgcttttca caatcagatt cgccccgaac agcatccccg ttttgctgta
     3841 ggtgacgctg tccggttgcg tgaagatcat ctcgtaactg gccaccattg catgcccgat
     3901 acgggtcgtc agtgaaagtg gcatcgccag accgaccggc acattatcca cccggtgaac
     3961 tttgaccagg tcactgttat caataatctg caccgatgtg cggacattaa ccagcaggct
     4021 gataagcaca aacacggcca cccagcccag caaatccatc acgttgtgcc gctgtaccca
     4081 cattacggcc accgcgagaa cagacaatgt gagcgcaatt ttttcaatgg aatcccacgt
     4141 ccaggttccc ataaaggcgg caatggcgtt caggttattc cgcaaccact caccaccggc
     4201 aatcacataa acttcattca cagcgtgctc cctccgaagt gatagttgtt ctggtaacga
     4261 ctgagcatgc gggcggaaag ctgctgacgc atgtagctca tctgacgatc gacaaccagc
     4321 agcgcatcct gctgtacctg cacctgtgac tgaaacgccg caatctgccg ggtggcatca
     4381 ttcatgttgt cgttaatatg cccgataacc gcttcgtcat aatttcccgt ggccaccatc
     4441 gcccgggcct gctgtatcag ctcctgaatg tactgcagca ggatgtcgta accgatatag
     4501 tccgtcagct ggtaaatcat actgttggaa acaccgagca tctgcgggtc aaccaggtat
     4561 ttgaagacgg ggatggtggt actggaaata aagccttttt ccttgtcatc cagaggcgtg
     4621 tcactgacag ccttgttctg aatgctggcc agaagtttag taatctgaga cttcagtgca
     4681 ttatcccggc tgatggtcac cggtgtgtct gccaccactt tcaggcattt atcagaatcg
     4741 ttgcagtgat aaatttttgc tgtgccgcct tccatcatgg cccggataat tgagcggtca
     4801 gtggttctgg ctgacagcgg tgtgatttcg ccgttgggac caaacaccag tgagccggtc
     4861 agcgtcatca caaactcttt cagctctttg ttgccgtcaa acattctgtt tttggaaagc
     4921 gcattccaca tgatgttaat gtttttggtc acccgctcct tatccttgtc gctggcttta
     4981 tccctgacgc tgtcagattt accgccaacg gtacatccct gccgggaagc cgcccagtca
     5041 gcaaaaatat tgctctcacc ggcaatgtcc tgacagactt tttgctggga cacctgcgtc
     5101 cgggggaaaa gcccgccgat aatcccctgc gccgcctggc aggaactgag gttcatgctg
     5161 ttaatgtcgc ttgccatttt ctgcaggaag tcttttgcgg ttttgatttc cggcaccgtt
     5221 gtctgcaggg caaggtcaaa aaagtaaccg gcagcattac tcataatctg cttaacaaaa
     5281 cgctgcagct gctcaccatt aataaaactg aacgaaccga gataagcgtc gatgccgcca
     5341 catccggcat tgatatccgg cagcgtcatg gaaatcagct gaacattttt gacctgagta
     5401 cgggcataca gggagccgcc gtaagcatag ccggcagcct gcccctgcca gacgcccggc
     5461 tgtgtggtat tggaggcgaa gcccagcttg ttaaagaact gattcatgtc gctgttcaca
     5521 tccgctgacg ctgcaggcgt caccgtcagt aaggcggcac aaagaataaa aagaggctta
     5581 atgcgaagca ttattgctgc cctcccttaa tgtgttctcc cggcgcatac ggcgtttcaa
     5641 caggaatatc ccgatatttc cgtatattaa ttcgtacatt gggaacagaa aaaatgtaac
     5701 aagaaacccg actgcaagcg catataacgc atacggaatg acatatatca ggatattcca
     5761 catataaatc gaattattta attcttggtc cattttccac gaatcaatac aggcatcaaa
     5821 aataatattc atagctgtcc ataaaacaat tccggcagtc agaaatacca gaggcaatcc
     5881 agcatacctg tcagtaattc tggaaatgta tttataatct cgttctgtac ttctgatttt
     5941 gagttcagga ttatttttat tttctctcat tataagttcc ctctgatatt tccgataatt
     6001 cattcagacc agtcctgcgg ctgtaatata tttcagacac cgaaatccat cccacaattg
     6061 cccccacaaa aaacacaaca gaaacagaaa aggtaaggca cagcgaaacg acaatttcta
     6121 cagggctggc gggataaaaa agcccctgat ggataaaggg gatattcagg ctgacgatta
     6181 ttcccgtcgc gatgccggat attccggcaa tgagtaacac cacctgctta tttcgcacct
     6241 tttttccctc cgtacatctg cagaacggta tccatccgcg ccataaaacc ggcggcatcc
     6301 gttgccccct gtaaaagcgg taatgcctca agcgtgttga cgttgaccag gaaggtggtc
     6361 ggtgtggcca ccggaatatt cgggaagaag gtctgcatca cgtccggtgg caccggtaat
     6421 gcttccggaa aggcggtatc cccctgacca tccagggtgt agggaaaaac ggaaaagccg
     6481 tactgctgtg ccagctgctt cagtaccggg tcaaactggt gacagtaagg gcaatgcccc
     6541 tgcataaaca gcaccacctt ccagtcggcc aggttaacct gcctgccatt actgaggcgg
     6601 aaccagcggg gagccggctt tgctgtcctg gctgacgtgc ctgctgccgg ttgtgcaggc
     6661 tgcgtagcca taccctgcgg attccagagt cgctctattt catcccgggt ggaggcctgc
     6721 acagcagcgg cgctcagcaa cagggtgaac agcagtgatt tagtgagaga catgtccgcc
     6781 ctcctgctgc ccttccatca tggcctgttg cctggcttcc agtgcctcac gctcttcccg
     6841 gctgacacgg gcaatccatg catccagcac ctggtatgcg ccgaacagta cgagaatggc
     6901 cgcaatcagc tccgccagaa aaaaagccat ccagggtttg ctgtaaacaa gacgggcgac
     6961 gatatgaaac cagacaccca gggaaaatac ccacattccc gtaatatcaa gccggggtaa
     7021 agagaatctg cgtttactta tcatgttaaa ctccggttat tatttacatg agagtattca
     7081 tgtctgtaat attcgcgttt gtgtgatatt aatcagaggt acaaatggaa aagcgatctt
     7141 ttaaagaaaa actggaaata atcagaaaca taataagaga gtcgttactg ggaaatgcgg
     7201 caattattgc gctgatatat gcagcatcac attcattacc ggtcaatgcc ttccctgact
     7261 atcttgtaat aagtttatta agtatcgcag cagtcatcgt tgtattatgg ttattttcaa
     7321 ttatctatat ttatttttgt gagctattca gaagccactg gattgcggta tggtttatca
     7381 tatggtcttc agtaatcaac ctcattattt tgtatggttt ttatgaccat tttatatgac
     7441 ttagcctgtc ataagccccc ttataaaaaa ccacatcact ccggaccata ccgatgacaa
     7501 cacaatacag ataaatattc ttctgaacct gtcgaagctc gtaacgtccg gtaatgcggc
     7561 cgcaactatt gaaacaacca gtgctgcgcc ctgactcata attgcaccac acagaaacca
     7621 gagactggcg gtaagcacat ataaacacgc gatattaata acaaggaaaa ccagtgagct
     7681 caggagaaac attttcctgt cattacgctc agaaaagaaa taaccggcaa taatgacaaa
     7741 cattaacaga acaacacctg taaacatttt caaataatct tcactcataa atcaaatccg
     7801 cgattaaaaa ttgggtttaa aatcttcaga aacgttcagg aactgttttg ccaggtcgtc
     7861 ctgcgaaata aagccgtatg ataacgggcg aacactgccc tgtttcgggt caaccagcat
     7921 catggccggg aaatatttca cgccgaggcg ctgcgcctgc ccctggtcag tccgggaatc
     7981 cggcaacagc ggattaatca cgccatccac ggaaacggga ataacactca gaccatacgt
     8041 atcccggaag ccattaatga cctgcgccag ttgcccgtcg atggggtcct gcccccggta
     8101 aaaaaacatg atgccgtagt gttcagccag tttcgcaatg gcctgtcgct gctgcgcctg
     8161 gtctgctgcc agctggttcc gtaccgtgcc gttgtaatgg ctgtactgca ggttatagtc
     8221 cagttcagga tgtgccagca tggcctttct ggcgctcatg gtgaaaagcc cggcctgctg
     8281 agtccagtaa ttttgcagcc ggaaatattt cacaaaatta tccacgccgg gatacagaat
     8341 ggcttcgtac agcgcccgct ttgttgccgt ctgcagtgcg gccagtttct gcataatatc
     8401 cggttcctga cggggggctg caggtacagg tttattttct ttttccttcg gatttatttt
     8461 ctcgttatac cactgccagc ctgcatcttt tcctgacgcc ggaaagataa agcagcagag
     8521 taacagtggc agtaatgctt tattcatctt cattcctttc atcacttttt ctttcatcag
     8581 gtttctgcag gtcagtcagg gcagaaaaac aaaaatacag aataaacagg agcacaccgg
     8641 agtaaaggat aacgaaagca gcggcaagca tcccgtctgc cgtggtttct tttaccgtat
     8701 cccacgacca gataattacc ggagaaatga caatcgctgt aatcaaaagc cggataagaa
     8761 aatcaataaa acggttcgac gtgaatatga ctttcatcat ttcccccggt tatttcccat
     8821 accttactgt cctgcctgtt tcagctggtc agcaatctgc tctttcactt tctgcgtcag
     8881 aacgccactg tccgggattt tctggttatt catcaggtct tcgtagaagt tagtgaaatc
     8941 cagtctgttg aactgaattt tctgcagctc atcaaccgta atcccccgac agtcaggatg
     9001 cttcgcacta ccaaaactga tacgcaactg accgttgcgc ccctgctgct ggactatctg
     9061 cgccagcttc gaatcaaact gacagtagct gcgttttttc tccagacaga cacccagcac
     9121 tttcttcgaa cagaactccc cgacgctgac ggtcagtttg tttgattttg ctttagccag
     9181 ggccttttca tcactgctgc atttggccag cccgatatcc tgtccccagc cgctgtcctt
     9241 acagcagttg ctgtatccgg ctgcagcctt cttacaaaat ttcgcctgac cggtgaaagc
     9301 acggacatcc accccgttca gcgccgcaac atccttacct gctgcggcca gtgccgccag
     9361 ctgagaaacg gcttcggcaa aatcattact ctgcccgctc tgagccttat cgcattcgcc
     9421 atcgagacag aagacgtctc ccccacagac catcactttc ccggatgttt ttgactcaca
     9481 ggagtacgtg gcatattcat gcagacaggc accttcttcg gagtaaaagg cacactggtg
     9541 tgacaccagg gtacaggcag gattgtcggt gtaggtctga caggttccct tgtctgctga
     9601 ctgcgtgaca tacgtgtccc ggtatgccca gcagctctgg tacatgctgt atgactgacc
     9661 gtctttcacc agcgtcctgt tcccgccagc ctccgtacat tctgtttttg tcagtttccc
     9721 cagctccttg ctgaacgggc agctttcaga ccagacaaca tacggggtgt acgaggcttt
     9781 ttttaccctg acaaccagac gcagcttcat tgccgtatga cgtttgaatt ttgtattccc
     9841 gacccggacg gggtgagatg acgtaaaagc cacaccggca gtcagtgttt tctgtactgg
     9901 tgtaaatgag gcatctgctg agtatttggc attccagctc agtggggttc ccagtactgt
     9961 aattgtcata ggtacactct tctgcaggta agtacgactc cagctgtaat gcgcatcgac
    10021 aatttcaccg gtaacgggag aggtaatgga gacaacatac tgaccattca cttcacgtgc
    10081 aggtagctga ctcatttcat actccagcgt ccgggtttcc catgtggtac tcccctgcag
    10141 ctccatacgg gcagttcgtg tgcagtactg ctccacctgt aaatcccgct cacaggtgta
    10201 attcgtgtat tcactcctgc taatctcctg cgcactgcac tgctgcccgg tgtttcccac
    10261 aatgctgtca gcccggttca ccacatccct gccggtctgg ataaacggtg catccggtga
    10321 aagaatgtct ttcggcttgt tcatgaatga ctctgtaatg gtttttccgg tttcaccggt
    10381 cgcccattcc gtggtgccgt catttttcag gccgccatcc ccgccggcag tcacgccacc
    10441 gtaatatttt gtctcgtccg gattcgcgtt atagccgggg atactctcct gtggcttaaa
    10501 gccctgaata ctgctgcttc cctgcccttt gatctggtga gcaaaatcag aaccggcccg
    10561 gtaatcactg ttgctgtcag cctgtgccat gccggcaacc agagccagta tcagaggtaa
    10621 aatacgtttc atttcccgga atctcctttc cctgccagta aatcatgagc cacctgccgg
    10681 cagtcacccg tggcggccac tttctccagc gcctggccga cccgcaggtt tccccggatg
    10741 atgtcgtatc cctgactgca gaacaccacc agtgccggta cagtgcgaat gccgtactgt
    10801 gaaaacagcg tcgggtcgat ctgaacacca tcagtcgcgc cgtctttcac cagggacaac
    10861 acagcttcag cggtggtctt caggtcattg ttcaccatgc cccgcagtgt ggccggaata
    10921 ccgtagtgcc gggtttcgcc cagcattcgt ttcagcccct cttcgggaat ggaaaacgac
    10981 acaaaataca gagcaccctg ccggggtttc ccgtcctgcg aagcctgctg tttacgaacc
    11041 agctcatcca ggaaatggtt atctgaacgc tgaagggggt tttccagcac ctgtttctcc
    11101 gcccaggctt tcagctgatg gtcaggtttt tcacgcagtt gtctgcttaa attttcctgc
    11161 tgcttcagga actggcggtt ttcaggagtg ttcacgtttt ctgacgccat aaccgccccg
    11221 ttcagcatca tcagcagtgc tgccagagat ttcatactca gcttcatcat tcacctcaca
    11281 ggaagacaca gttacgttta cgccacatca ggtagccgaa gtttttcttt gtgttgggcg
    11341 gatttttccc ggtttcccag cgggtcacgc tgcgcccgaa cgggtggcac tgcccgctgt
    11401 ccgggtacat attcaccatc tggtaacgcc agcgttcttt gggcaggatt ggggacggat
    11461 attcattaca gacggcgtta tttttcccga tggtttccat aatcatgccc tgacggtgca
    11521 gcttgaacgc catgcgttca ctgaccagca gggaggactg caacggactg gactcattac
    11581 tcacccagcc attgaacggg tacatactgc cctgcgaacc ggcacaccag aacagaacat
    11641 cgagaggcat attaaaggcg ctggcaatcg catctgctgc acaggccccc tgtgctatcg
    11701 gattggcaaa gatgacagct tccggattga gaatggtggt caggctgctg tccgtccagg
    11761 tggggtcgat ttcagaaaga taagcgatat ccaggtcacc accttccaga cagcccagcg
    11821 atgtgatgat gttaagccag tacgtcagcg ggtatttgta ccagtgaacg tgatagaacg
    11881 ccccgttcac ctgcttctca tcctttttcg ccgttccctg tgccgtttta ccaaaagccg
    11941 gcaggctgaa gcccaggttc accatgcatc ccggtgatcg ggtgacgtcc gtcagcgcca
    12001 tcggctccca gtaaccaatg gccagcccga tacgcctgaa cagcggcggc ggtgccggac
    12061 aaatctgaat gggcatcgac gggttcgccg tgtcggggac cttaccctga ctgactttga
    12121 tactgcccag cgagagcggg aaaatacagc tccagcagat atccgtgatc gggtttacaa
    12181 aacgcccctc acaggcagaa tccgcagacg ctgcagggac atgaccggcg agaaggaata
    12241 acatcagcag ccacaggctt cgcttcattt tctgccctcc tctgccggaa taaattccac
    12301 cttcaggaag cgatcgccgg gaacggcgct cacccgtgcc gggacctgat caatgccgag
    12361 gcgctggcag agcacaccgt tctggtcaaa gtaaacacgg ctgtccagag acttctgcat
    12421 ctccgggata ctgccctgca cgaggataat tttgctctcc agtatgggcg gcgtctggcg
    12481 tttcatccag gccacctgcg ccggatcatc gccattgatg aaatacagcg tctggttaaa
    12541 aggaacatac tgcagcggat tcatcacttc accctgacgg gcaaacaccc gcccttcgtt
    12601 atcccggata tccgcagcca gtctgacgga cgggtcaaac aaccggctgc cgtatttctc
    12661 cgtgcgtccg ataccgggca cggcaggggg ccgcagactg ttgcggatca cccgctcctt
    12721 aaaggcatcc attttccggc ccatctcacc ggactgctcc agtgccgtca ggcgctgcat
    12781 gatcaccgtc agcatgtccg gctcctttac cggccacaaa tcgccccagg taccaagatc
    12841 ggcggcggcc acactctgcc cccatatcag cagggcaatc agcccccggc atctcatggt
    12901 tccgccctca ttcgccgggc aatatcctgc tggatttccc gggtgatatc cggtgccccc
    12961 tgtaccacag caggcgacac cagaatgacc gcgtggtgtt tctgctgcca cgcctgcagg
    13021 ctggcttcca gtgccgtgtt aaaacgcgct gaaagggctt ttgactgcgc ttctgacagt
    13081 tgtttctgac tggcgctgtc aaaaaacgca tccacggtct gtttcatgtt gaaggcgaca
    13141 gtcaccggtg cgttcagtct gacaatgccg taactgacgg ctgcgttcag taacaccatt
    13201 gccaggcatc ccggcaccgt ccaccaccag tggctgcgtc gttctgccgt gacgtcggcg
    13261 ggtttctgcg ttgaactcat gccacactcc tgtatttctc atgttcttcc agccaggctt
    13321 ccagcgaagc catttccggc cctgacttct tccacgccag ctgccacact gcctcatgaa
    13381 tactcagccc ctctttacgc ttctgctgca caaactcaaa atccgggccg tcagagctgt
    13441 acatggcgcg gcttaacggg tccacaaaca gacggtgcca ggaggaatgg ttttccacct
    13501 gcagcaggaa ggaactgaac cactggtctt tggccgcacc aaacttacca atcatgtcgc
    13561 gctgcagtgg caggaactgg tccggataca gttggttgta tttcgcgaac tccttcgcac
    13621 tctgtttgag gataattttg taggaggagt taccccatgc cgcgcgggca gcactggagg
    13681 ccttatcaga gtcaaagtcg acgatgttct gtgtgatggt gatataggca ccggtatgac
    13741 ggcgggccgt acggtagccc ttctcaataa attcaccgac cttgtgattt ttgaagtcca
    13801 gcagacgcca gccttcatca ataacgttca gtttcttgag gttacgcggc gtgcggtaca
    13861 tcctgttctc gatgtagata atcagggaga acatcaccgc aaccagcagt gacggacggt
    13921 cttccagtcc gcccagctcc agcaccacca tttttgcgtc atcccgcagg gacggctcat
    13981 cagagttaaa atactggccg taagtgccgt tggcagtgta ctggtcaagc agcacaatca
    14041 tttcgtccag acggctgcgg atagtcggtg actcggcata ctgctcgctg tcactggcgt
    14101 ttttcaggaa atccaccacg tcatcaatac gtgctctgtt ctctttggcc agccaggagg
    14161 ctctgaccgc ctgcaacagc agaccttcat gcacttcatc caggttaccg ttggggctgg
    14221 ccatcactga caactggtca cggacacgct ccgctgactg gtcaatatcg gtgatgttcg
    14281 caaacgggtt aaaacgcagg gtttcaccgt ccagatacac cccgcccatg ttctcacaca
    14341 gggacttgta tccatccccc atgtcgaaca ccacggcaaa gcctccggag tccagcacgc
    14401 tgcggataag tggctgtatc agcccggttt taccggcccc ggaggtgcca cagaccgcca
    14461 tgttgtagtt ggtgttattc atcccccgga agaaaatatc gataaacgcc agctggttac
    14521 ggtaggtggg tgccagcaga cctgccggtg tcagggggtt atccgccact aacggcatca
    14581 ggttggcaac attaaagctc tctgcgcgct gaaccactcc ggcctctttc agctgtttaa
    14641 acagcccttt cccggccata aagggcaggc aggtcaggaa attgcgcatg tggttaaagc
    14701 gcggtgaaat cagctcaaaa ccgtttttac gaaagctgtt caggatgtcc tgctccactt
    14761 ccagtgccgt ttcattattg tccttgcaga aggctgtgat gttgaggaag taggacacga
    14821 cagaggactg accggagccc agccgctgac gcagttcccc ccactccttc gcctctttct
    14881 ccacggacgg aaaccatttg gcataggagg tcttcgactt tttctccagg tccatgtact
    14941 tcaggttggc ttcgctgtgg gttttcacct ggtcttccac caccagagtc agcgtcagga
    15001 tgaacggaca ggagatggac agttccgggt tcagcaggtt gctgtagttg tcggccatgt
    15061 tccacaggaa ggcgatttcc gggttacggg ccagatggaa attcaggatg cgggccgtgc
    15121 tgttcctgcc gttctcacgc aggcccagcg tcaggtaatc agcccggact ttcagatcaa
    15181 aactgtcctc cacacactga taattcagat cagaatacgg gtccagctga cgtcttttcg
    15241 ggtacaggga atccgggtta tggttaatca tctccccgac aatatcgata aaggcctgtg
    15301 catccaccgt ctgtgtggtg atactggccc cctgtaacga cgcccggatg attttcacca
    15361 ggttttccat ttccagaatg tcggcccggc tttttttctt cgagggagaa cagtacgaga
    15421 taaacacccg gtaatggcgc agggtcaggg gcagattcat tccctccggc agcggaaact
    15481 gtgtcgctgc cgctttcata taataggccc gggtaatggc gttaaaccgt tcagcctgtt
    15541 cacctgacca ggagaactca cgcagcccgt attcaatcct gtcaccaacc agctgactgg
    15601 acatcagatg gatacacaac ggaataccgc gcggcagttt ggtgcgcaac atgtgatcga
    15661 gggcctccac aatggactca ttcgccccat tgatggggat ggcttccagc ataaagccca
    15721 tcgtggtgtc attcatgaac agaccggatt cctggttgta atcacggtac ggcagcagac
    15781 ggctgaactg cgggaagctc atttcgccca gaacttcatt ggcctttgcg gattcgtcag
    15841 gcagtttcag tgctgtgacg agggagttaa ccgcctgagt gacggcctca agtgggttat
    15901 tcacatgaat tccttagttg aaaaataaag cgagaaatgc ggtagccatg aagggaatac
    15961 ctgtaattaa cgcgccattt cccggcgcat atacaaagat ttctgttttc tgaatatcca
    16021 ctcatgttca tccggaatta aatcactatg ttctgcgact gacttatgca taatgttttc
    16081 tctgtctttc ctgatacgcc tgacattcaa cgcacaacgt cacaccggga aatattttcc
    16141 gccgggcttc cggaatagga tttccgcatg cttcacagag ataaacagga ataccatgag
    16201 catttatttt ctggcgtatc cggtttattc ctgtcatggt cagttgttct gtcactgaat
    16261 atgcttcatc ggcttcatca ctcacggaat attcctcccg acatgttctg acacgctacc
    16321 gcccatccct tttcacgcac agggcactcg ttttacaggc agattttgaa tacagaaagc
    16381 ccgccggcat tgccggctga tatacaactt cagggccagt taattaatac gtggttttcc
    16441 ccacgcagac ggtttaataa caaaaaacac gctggatggc tgatgataaa catcctggtt
    16501 atcaatataa ggcgctatcc ataatgctgc cgtctgctca cccgttctta gcggacgagg
    16561 aggtgttacc ggcgtaactg aactgaccgg aacaaccgtt ttcacttccc gggcagcggt
    16621 aaacagcggg cgaggtgcca gcagtttctg ctccggatgt gctgtcaccg caggtctgct
    16681 gccggacgga gtggtcgcgg tgacggtctg caccggcatt gtccggaagt tcccttcagc
    16741 caggcgcggc agtgatgccg caaccggctt tgcttctgag gactgctcca gttttttggc
    16801 cttctcattg gcctgctcca tcgtcataca ggtatcggat gtggtggcgt tacactcaaa
    16861 ttccgtactg gttccggcac agccggaaag taataaggtc cccagcagag gaataaagaa
    16921 agaaatctgt ttcattgtcc ctcacctttc tcatcgcagt tcacaaaatc cttcttcaaa
    16981 tccctgaccg tacaggtttt tacaatgtca ggattttcaa aaaccgtact ccgtgatgat
    17041 ttataaacat atgccacaat gcgtttccca cccgcatcag gttctggtgc actgacttca
    17101 cataacggag gtgttattcc ggactcatac agcaccggat aatttatttt tttgacgctg
    17161 ccatcagaaa ccagcttatt catcgaaggg ctccttatat ttctgaataa ttctgtcgat
    17221 tagttctttc accggaacag attttccgcc accggattgt ccggcaacca caatatgccc
    17281 cggagaagaa aataaactca tcgacatacc cttcttcatc acaccgacag cagccatatt
    17341 aaaattgtcc ggcaatgact cagaaccgaa tttctccggt gcaggaagta cgattcttgt
    17401 ggcgcagtta ctgagaacag catgcataaa atcatctgac acggttttac ccggcacaga
    17461 gagggctgta ataacattaa tattacgcat gttcattttt atctccgttg tttctgtttg
    17521 ttctgtcatc aggaagataa acagggcatt tcctgatttt tcccggcagg gaagcgtctt
    17581 ccgtataaac ttccttcaga tgttcatctg cggttaccac ccggatacat ttcatttttc
    17641 cgggtcgcac cggatatttc ccggtgatcg ctgccagcga gacatacatc agtgccagtg
    17701 ttgcccataa tgcggtgacc agttgcatca gtgaccggtc agtctccctg acataatctg
    17761 cagcatcagc cgcgcggtaa tggcagatat cctgtatttc tgatctgcct gaagaatcag
    17821 aggaagaaaa ccagcattca ctgaccggga actgtccggc aggtgctgtt tgtcttacga
    17881 agttaatctc cttaaccagt aatttccccg gcgtggtgtt gtccttccat aaaacaaaca
    17941 gagccatcag ggccattgtg gccagtggca gaatgacggt gtacttcaga caccagaaca
    18001 gtcctcgcag aacccgggcg acaacatacc gggcggcatg gcacgcctga cgggacgaca
    18061 tattatttgc catcgttgcc ccctccctgg ctgccggaca taaactgctg cggattcagg
    18121 ttgcccagct gcttcagcat ctggtcagta ttaaagccgt taagatgact ttcagccgat
    18181 ggcggaacag gaaccggact ttccggattg tcttcttccg ctctgctctg cgtgcgttcc
    18241 agcgccatct cttccacggt tttcagctgg aagccgtcct ggaacaccac ggtcacttcg
    18301 ttgcccgcac caatcggtat caccgggtga tactgttcgg cacgtttgat gtagtagtca
    18361 ctgagcgtct gtgcggcttt cgatgcgccg ccaccgatac ccattttcag gacatcacca
    18421 gccccgtaag cggctgtggc acccagcccg acagccggct gggaggcgcg ctccattccc
    18481 tgaccgatac cgtcaacaaa tcccgcgccc catgcccagc cgaggatttt gccgttacgc
    18541 atcaccactt cgcccttgat accgttttta ccccggaagc tgacatgccc cttaatcggc
    18601 atatcaatag ttttgccgtc cttcaggcaa ctgatattgc gggtgcgtac aatggcacgc
    18661 tcactggaca catcccccca ggcttccaga cccacaaaac atcccgttgc gtcatacgtc
    18721 ttgctgttcg gcatttccac caggccggtg atacgcagct gcatcggcac cgtggattca
    18781 ttaccggtga cagaggcatt ggcatccgcc ccttcaatca gcatggcttt cgcaaaactt
    18841 cctgacggaa tgtacggcag tgatggtccc tgttttccct cattacgggt aaacacctta
    18901 cgctgtatcc ggttaggcac cggcacagac tggtacgtca cctgtggtgg tggcgtgaca
    18961 ccattcccgg gataaaacgc cgtcggcggt ggaacagcaa cgctgccctg cggcgggaag
    19021 gatacggggg tgtttcctgg ctgtggttcg ccttccgggc cgggcggtga ggcaggcatc
    19081 tgcggtacag gctcaccggt cgccgtgacg ggattagcac ccagggcttt tacctgctct
    19141 gccagggcgg cattgtcctg tcccaacttt tcaatacgac gctggtcatc accgcgctgt
    19201 ttgttcagaa catccagctc acgacggatt tcctcatact gcttctgcat ctgcgctgcc
    19261 gtcacctgca tctcggtggt ggcatgctga cgcaccttgt catcaaaggt cgtatccacc
    19321 acaccggtca tatccggcac aggctcctgt tcagccacgg tttcaccgtt accggacatg
    19381 tccacatcag acagatacag tgccccacca atcgcggagg ctgtaccgac aaccacaatc
    19441 cccagccaca ggtactgctt gcgtttcaca atggtattga tactggccat cagttgccct
    19501 ccccgtcacg gatgacatac acatccatgc gcgcacctgc cagtaactgg ctggcaggct
    19561 ggctgaacat cacggcacgg gttcccggct gccagaagtc actttcccgg atattcaggg
    19621 cagacagcgt tttattttcc acggcaaagc ggaccatctt caggtgattc cccgtccata
    19681 cggcatctgc cactgaagac agccccgccg gggcctgcag ggtttccttt gtcactggga
    19741 cctgatacca gcctgcgggt aattttccgc cacggacggc ctggctgatg gtcacaagca
    19801 gggattcgta aggcgtggac gtttcccacg caccggcttc ttctccggtt ccgcgcaggt
    19861 cactgaccag ctgaatggta cgccccgcgc cttcacgggg aacggcctga atggaaagat
    19921 tcagaccacg ttctgtttcc agaatgaacg taaagggctt tttgttgacg gtggcaacca
    19981 ccacaccgcc ggaggcggtc tgctcattat tggtcagggc accatccaga ctgttcacgg
    20041 cgataatccg gtcaccggga acggtaaaca gattcgggct ggtattgctg atggcaatac
    20101 tggcctgacc accgttcacc attggcacca cggtgggggc cagcgtaccg tttgcggcca
    20161 tcacgctgca ggaaaaaaac aggctgccga atattattgc cgtattattt tttctcatcg
    20221 tctgtttccc cgaaattatc cagccaggtc accccgtttt cccgcttcag aataaggatg
    20281 taatgtttga tatcagtgaa aggttttgaa tcaccaatcc aggttttaag cacacctcga
    20341 atttccacac ggccatactg aggccagaca cgaacactgg tctggaaaaa ggctgagttc
    20401 acgttatcgt ttttaatacg cttcgcttct tcagccagaa taactttcat ctggttctgt
    20461 gcgcccgggc ggatatattg cagaagcgcc tgatgtgagg catcggcggt ttctgatgaa
    20521 acattcagac ggagggcaat aaatgacagc gccatctgct gtaaataaga ggcgtcggca
    20581 ctgttctgtg acacggcaaa gggggcatta aatgccattg gcgtcacggc agtgcgttgc
    20641 tcattctgaa gacggtagtt attcaccccc taaatgacgt taacagagag gctgagaaca
    20701 ataagcactg acataaatat aaaggcgatg gccattacac gactggtact taaacgggca
    20761 ccgtgttcca tataacaatc ctggtatcag ttctatttaa tccactgccg gaaacacgaa
    20821 tcgggaacat tatgaaaaat accgcgcagc agggctgttg gcatatacca gtaaatcagg
    20881 tcacgtaacc aggaactgcc ccgccctttt ttcagttttt taatcccgaa ataaaccaga
    20941 accgctgcac caataccgaa cagatatttc gatgttgtga taccccagcc aatacagatt
    21001 gctgcgggga tcagttcatc cagtggcagg ccaaaccagc ggctctggtt ggtcagtgtt
    21061 tccgggaaac gatatttctt aagtttattc tcgtctcccg acatcgtttt atttcctgtc
    21121 agaggccaac gacggccata cccacagcaa taaatacaga gatgatggca aaaccggcca
    21181 ggaacttgac gtttttggtc atcatgtaca tgaccgcacc gaccagaact tcagccagaa
    21241 caacccattt aacaacactg gagtccttac cgaaggtcgc cttaaccgtg gtgttaccac
    21301 ttgccatcag gtcctgacca ctgctgccgg cggccatcgc cagctgcggg aagaacatca
    21361 tcagaacagc agccgggatc acagcgcgag ccaggcgaag catattcaga cgagtgaatt
    21421 tagaaaaaaa cgacttcttt ttgacgggcg cagaaacacc ctgaacactt aaaacggcgt
    21481 ccatatgatt cctctgttta atattgatag tgaaaaataa aactctttat atatcgtcta
    21541 tgctttcagc ttcctccttt actaaagatg cattataaaa gtctggaaaa cgattgagat
    21601 gatcttttag tctaatgaac acctcattag tttttgttct tccgctacga tctctagctg
    21661 ctaaaagttt ctgattggtt gtctcatcca gtattaataa gactttgtta cccggtgcgg
    21721 ctctggttct ccctctgctc acgtttcctc c
//
LOCUS       DAZFQD010000082        21628 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.082, whole genome shotgun sequence.
ACCESSION   DAZFQD010000082 DAZFQD010000000
VERSION     DAZFQD010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21628
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.082"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..443
                     /gene="decR"
                     /locus_tag="ACHKTW_003123"
     CDS             <1..443
                     /gene="decR"
                     /locus_tag="ACHKTW_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HFO6464096.1"
                     /translation="RKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLEDDGILIG
                     KVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGFWRMAGEYDYLMR
                     VQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            473..2245
                     /locus_tag="ACHKTW_003124"
     CDS             473..2245
                     /locus_tag="ACHKTW_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HFO6464097.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGQILMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVAQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIHQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWEQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDELAQQSGWYRD
                     MYRYQQLEAALDDAPEIREEAVDA"
     gene            2238..4019
                     /locus_tag="ACHKTW_003125"
     CDS             2238..4019
                     /locus_tag="ACHKTW_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HFO6464098.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKMVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGVVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDER
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIRLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDGIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIVDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            4200..4538
                     /gene="glnK"
                     /locus_tag="ACHKTW_003126"
     CDS             4200..4538
                     /gene="glnK"
                     /locus_tag="ACHKTW_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFO6464099.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            4568..5854
                     /gene="amtB"
                     /locus_tag="ACHKTW_003127"
     CDS             4568..5854
                     /gene="amtB"
                     /locus_tag="ACHKTW_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HFO6464100.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSAGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(5902..6762)
                     /gene="tesB"
                     /locus_tag="ACHKTW_003128"
     CDS             complement(5902..6762)
                     /gene="tesB"
                     /locus_tag="ACHKTW_003128"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HFO6464101.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHGIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            6980..7552
                     /locus_tag="ACHKTW_003129"
     CDS             6980..7552
                     /locus_tag="ACHKTW_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HFO6464102.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINKGGTKADLTLVPVQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            complement(7583..7894)
                     /locus_tag="ACHKTW_003130"
     CDS             complement(7583..7894)
                     /locus_tag="ACHKTW_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HFO6464103.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            8057..8153
                     /gene="ffs"
                     /locus_tag="ACHKTW_003131"
     ncRNA           8057..8153
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACHKTW_003131"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            8273..8626
                     /locus_tag="ACHKTW_003132"
     CDS             8273..8626
                     /locus_tag="ACHKTW_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 family protein"
                     /protein_id="HFO6464104.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAATQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(8668..10218)
                     /gene="pdeB"
                     /locus_tag="ACHKTW_003133"
     CDS             complement(8668..10218)
                     /gene="pdeB"
                     /locus_tag="ACHKTW_003133"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HFO6464105.1"
                     /translation="MRTRHLVGLISGVLILSVLLPVGLSIWLAHQQVETSFIEELDTY
                     SSRVAIRANKVATQGKDALQELERWQGAACSEAHLMEMRRVSYSYRYIQEVVYIDNNV
                     PQCSSLEHESPPDTFPEPGKISKDGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPAS
                     FIDVIPYSSWQIDAAIIGNAHNVVITSSDEIAQGIITRLQKTPGEHIENNGIIYDILP
                     FPEMNISIITWASTKMLQKGWHRQVFIWLPLGLVIGLLAAMFVLRILRRIQSPHHRLQ
                     DAIENRDICVHYQPIVSLANGKIVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPL
                     TLLIIRSVFEDMGDWLRQHPQQHISINLESTVLTSEKIPQLLREMINHYQVNPRQIAL
                     ELTEREFADPKTSAPIISRYREAGHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVD
                     ALEYKNVTPHIIEMAKTLKLKMVAEGIETSKQEEWLRQHGVHYGQGWLYSKALPKEDF
                     LRWAEQHL"
     gene            complement(10382..10852)
                     /locus_tag="ACHKTW_003134"
     CDS             complement(10382..10852)
                     /locus_tag="ACHKTW_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HFO6464106.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            complement(10968..11519)
                     /gene="maa"
                     /locus_tag="ACHKTW_003135"
     CDS             complement(10968..11519)
                     /gene="maa"
                     /locus_tag="ACHKTW_003135"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HFO6464107.1"
                     /translation="MSTEKEKMIAGELYRSADETLSRDRLRARQLIHRYNHSLAEEHT
                     LRQQILADLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     APGVHIYTATHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKDVPDNVVVGGNPARIIKKL"
     gene            complement(11691..11909)
                     /locus_tag="ACHKTW_003136"
     CDS             complement(11691..11909)
                     /locus_tag="ACHKTW_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HHA domain-containing protein"
                     /protein_id="HFO6464108.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(11935..12309)
                     /gene="tomB"
                     /locus_tag="ACHKTW_003137"
     CDS             complement(11935..12309)
                     /gene="tomB"
                     /locus_tag="ACHKTW_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HFO6464109.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQDLQKWRK
                     SGNRLFRCFVNATKENPASLSC"
     gene            complement(12855..16004)
                     /gene="acrB"
                     /locus_tag="ACHKTW_003138"
     CDS             complement(12855..16004)
                     /gene="acrB"
                     /locus_tag="ACHKTW_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease AcrB"
                     /protein_id="HFO6464110.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWFNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSIPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
     gene            complement(16027..17220)
                     /gene="acrA"
                     /locus_tag="ACHKTW_003139"
     CDS             complement(16027..17220)
                     /gene="acrA"
                     /locus_tag="ACHKTW_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HFO6464111.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQEYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            17362..18009
                     /gene="acrR"
                     /locus_tag="ACHKTW_003140"
     CDS             17362..18009
                     /gene="acrR"
                     /locus_tag="ACHKTW_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HFO6464112.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            18137..21499
                     /gene="mscK"
                     /locus_tag="ACHKTW_003141"
     CDS             18137..21499
                     /gene="mscK"
                     /locus_tag="ACHKTW_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HFO6464113.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRIKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
BASE COUNT         5179 a         5389 c         5717 g         5343 t
ORIGIN      
        1 accgtaagct gctggcctta ctgcagcagg attgcaccct ctctttgcag gcactggctg
       61 aagccgttaa tctgacaacc accccttgct ggaagcgcct gaaacggctg gaggacgacg
      121 gtatccttat cggcaaagtc gccctgctgg atccggaaaa aataggcctc ggcctgaccg
      181 cttttgtgct gataaaaacg caacatcaca gcagcgaatg gtattgccgc tttgtcacgg
      241 tggttaccga aatgccagaa gtgctggggt tctggcgcat ggctggtgaa tacgattatc
      301 tgatgcgcgt ccaggttgcc gacatgaaac gctacgacga gttttataag cgtctggtaa
      361 acagcgtgcc ggggctgtcg gacgtcactt ccagcttcgc gatggaacag attaaataca
      421 ccacttcttt acccatcgaa taaatatcca gaatcaggtc aggacacaac gcgtgcgatt
      481 atttgctcaa ttaagctggt atttccgtcg ggaatggcgt cgctatctcg gggctgtcgc
      541 cttgcttgtc attatcgcga tgctgcaact ggttccgcca aaagtggttg gtattgttgt
      601 cgatggcgtg acagaacaac actttactac cgggcagatc ctgatgtgga tcgccaccat
      661 ggtgctgatt gccgttgtgg tttatctcct gcgttacgtc tggcgggtat tgctgtttgg
      721 tgcgtcttat caactggctg ttgaactgcg tgaagattat taccgtcagc taagccggca
      781 gcatcctgag ttttacctgc gtcatcgcac gggtgatctc atggctcgtg cgaccaatga
      841 cgtcgatcgc gtcgtgtttg ccgccggaga aggggtgctg acgctggtgg attcactggt
      901 gatgggctgc gctgtgttga ttatgatgtc tacgcaaatt agctggcagt tgaccttatt
      961 tgccctgttg ccgatgccag tgatggcgat catgatcaag cgcaacggcg atgccttgca
     1021 tgaacgcttt aagctggcac aggcggcgtt ttctagtctt aatgaccgca cccaggaaag
     1081 cctcaccagt atccgcatga tcaaagcctt tggtctggaa gatcgccagt cggcgttatt
     1141 tgccgcggat gccgaagata ccggcaaaaa aaacatgcgg gtggcgcgta ttgatgctcg
     1201 tttcgaccca accatctata tcgcgattgg tatggcgaac ttgctggcga ttggcggcgg
     1261 tagttggatg gtggcgcagg gcagtttaac gctgggccag ctcaccagtt ttatgatgta
     1321 tttaggtctg atgatttggc caatgctggc gctggcatgg atgtttaaca ttgtggaacg
     1381 tggtagtgct gcgtacagcc gtattcgtgc gatgctggcg gaagcgccgg tggtgaacga
     1441 tggtagcgaa cccgtgccgg aagggcgtgg cgaacttgat gtaaatattc accagttcac
     1501 gtatccgcag actgaccatc ctgcgctgga aaacgtcaat ttcgccctga aacccggtca
     1561 gatgctgggt atctgcgggc cgactggttc cggcaaaagt accctgttgt cgctcattca
     1621 gcgtcatttc gacgtcagcg agggggatat tcgctttcat gatattcctc tgacgaagtt
     1681 acaactcgat agctggcgta gccgcctggc ggtagttagc cagacaccgt tccttttttc
     1741 cgacaccgta gcgaacaaca tcgcgttggg ttgcccgaat gccacccagc aagagattga
     1801 gcatgtcgcg cggttagcca gcgtacatga cgatattttg cgtctaccgc aaggttacga
     1861 tacagaagtg ggcgagcgcg gcgtgatgct ttccggtggg caaaaacagc gtatctccat
     1921 tgctcgagcg ttattagtca acgcggaaat cctcatcctt gatgatgcgc tttcggcggt
     1981 ggacggacgc acagagcacc agatcctgca taacctgcgt cagtgggaac agggaagaac
     2041 ggtaatcatc agtgcccatc gcctttcggc actgacggaa gccagtgaaa ttattgtgat
     2101 gcagcacgga catatcgccc agcgtggcaa tcatgatgag ctggcgcaac aaagcggctg
     2161 gtatcgcgat atgtatcgct atcaacaact ggaggcggcg ctcgacgacg ctccggaaat
     2221 tcgcgaggag gccgtcgatg cgtagtttta gccaactgtg gccgactctc aagcgtctgt
     2281 tagcgtacgg ttcgccgtgg cgtaaaccgc tggggattgc ggtcctgatg atgtgggttg
     2341 cggcagcggc agaagtcagt gggccgctgc ttatcagcta ttttatcgac aatatggtag
     2401 cgaaaaataa cctgccgttg aaaatggttg cagggctggc tgcggcgtat gttgggctac
     2461 aactgtttgc cgccgggcta cattacgcgc agtcgctgct gtttaatcgg gcggcagtag
     2521 gcgtagtgca acagttgcgt accgacgtga tggatgccgc attgcgccag ccattaagcg
     2581 agtttgatac ccaacccgtc gggcaggtga tttcccgcgt cactaatgac actgaagtga
     2641 tccgcgatct ctacgttacc gtagtggcaa ctgtcctgcg tagcgccgca ctggtgggcg
     2701 cgatgctggt ggcgatgttc agcctcgact ggcgaatggc gctggtggcg ataatgattt
     2761 tcccggtggt gctggtggta atggtgatat accagcgtta cagcacgccg attgtccgcc
     2821 gtgtgcgcgc ctatctagcg gatatcaacg acggctttaa cgaaatcatc aatggcatga
     2881 gcgttatcca gcagttccgt cagcaggcgc gatttggcga acgtatgggg gaggccagtc
     2941 gttcacacta tatggccagg atgcaaaccc tgcgcctcga cggttttctg ctgcgtccgc
     3001 tgctgagtct gttttcatcg ctcattcttt gtggcttgtt gatgctgttt ggcttctccg
     3061 ctagcggcac cattgaagtg ggcgtgctgt atgcgtttat cagctatctt gggcgactta
     3121 acgaaccatt aatcgaactg accacgcaac aggcgatgct gcaacaggct gttgttgctg
     3181 gtgagcgcgt gtttgaactg atggacggac cgcgccagca atatggcaat gatgagcgcc
     3241 cgttacagag tggcaccatc gaagtcgata acgtgtcatt tgcttatcgc gatgacaatc
     3301 tggtgctaaa gaacattaat ctctctgtgc cttcgcgtaa ctttgtggca ctggtcgggc
     3361 ataccggcag tggcaaaagc accctcgcca gtttattgat gggctattac ccgctaacgg
     3421 aaggtgagat tcgccttgat ggtcgtccat taagttcgct aagtcacagc gcgctgcgcc
     3481 agggcgtggc aatggtgcag caagatccgg tggtgctggc ggataccttc ctcgccaacg
     3541 tgacgctggg gcgggatatc tccgaagaac gcgtctggca ggcgctggaa actgtgcaac
     3601 tggcggagct ggcgcgtagc atgagcgacg gtatttacac gccgctgggc gagcagggga
     3661 ataatctctc agttgggcaa aagcaactgc tggcactggc gcgcgtgctg gtcgagacgc
     3721 cgcaaatcct gatccttgat gaggcaaccg ccagcattga ctccgggact gaacaggcga
     3781 ttcaacatgc tctggcggcg gtgcgtgaac ataccacgct ggtggtgatt gctcaccgct
     3841 tatcgaccat tgttgatgcc gacaccattc tggtgcttca tcgtgggcaa gccgtggagc
     3901 agggcactca ccagcaactg ctggcggccc agggacgcta ctggcagatg tatcaactgc
     3961 aacttgcggg cgaagagctg gcagccagcg tgcgtgaaga agaatcattg agcgcctgaa
     4021 tagcgcaata tttcatcgtt ggtgcaaaaa tgtaacgcac tgtgcactgt catagtgcgt
     4081 tttcattttc aatcttctta acttcctgct ctctttctcg tttttcattt ctggcacacc
     4141 gcttgcaata ccttcttcgt gtagcagaac cattaccgaa ttctgaccgg aggggatcta
     4201 tgaagctggt gaccgtgata atcaaaccat tcaagctgga agacgttcgt gaagcgttat
     4261 cttccattgg tattcagggc ctgaccgtca ccgaagtgaa aggtttcggg cgtcagaaag
     4321 ggcatgccga gctgtaccgg ggggcggaat acagcgtcaa cttcctgcca aaagtaaaaa
     4381 ttgatgtggc gattgctgat gaccaactcg atgaagtgat cgatatcgtc agtaaggcgg
     4441 cttacaccgg aaaaattggc gacggcaaaa tcttcgtcgc tgaattgcaa cgcgtcattc
     4501 gtatccgtac cggcgaagcc gacgaagcgg cgctgtaatc tctggcacac agcaacagga
     4561 acgaaaaatg aagatagcga cgataaaaac tgggcttgct tcactggcga tgctgccggg
     4621 actggtaatg gctgcacctg cggtggccga taaagccgac aatgcgttta tgatgatttg
     4681 tactgcgctg gtgctgttta tgactattcc ggggattgcc ctgttttacg gtgggttgat
     4741 tcgcggcaaa aacgtgctgt cgatgctgac gcaggtgacg gtgacgtttg cactggtctg
     4801 tattctctgg gtggtttacg gttactcgct ggcgtttggt gagggcaaca acttcttcgg
     4861 caacattaac tggttgatgc taaaaaacat cgaactgacg gcggtgatgg gcagcattta
     4921 tcagtatatc cacgtggcgt ttcagggctc gtttgcctgc attaccgtcg gcttgatcgt
     4981 tggggcgctg gcggaacgaa tccgcttctc agctgtgttg attttcgtgg tggtatggct
     5041 gacgctctct tacattccga ttgcgcatat ggtgtggggc ggtggtttgc tggcttctca
     5101 cggtgcgctg gatttcgcgg gtggcacggt ggtgcatatt aacgccgcaa tcgccggtct
     5161 ggtgggcgcg tatctgatag gcaaacgcgt gggcttcggc aaagaggcgt ttaaaccgca
     5221 caacctgcca atggtcttca ccggtactgc cattctctat attggttggt ttggctttaa
     5281 cgccgggtca gcgggcacgg cgaatgaaat cgcggcactg gcatttgtga atactgtggt
     5341 cgcaacggcg gcggcaattc ttggctggat cttcggtgaa tgggcgctgc gtggtaagcc
     5401 ttctctgctg ggagcgtgtt ctggcgcgat tgccggtctg gtcggcgtga cgccagcgtg
     5461 tggctacatt ggggttggcg gcgcgttgat tatcggcgtg gtagctggtc tggcgggctt
     5521 gtggggcgtt actatgctca aacgcttgct gcgggtggat gatccctgcg atgtcttcgg
     5581 tgtgcacggc gtttgtggca ttgtcggctg tatcatgact gggatttttg ccgccagttc
     5641 gctgggtggc gtgggcttcg ctgaaggtgt gacgatgggc catcagttgc tggtacagct
     5701 ggaaagcatc gccattacga tcgtctggtc cggtgtggtg gcatttattg gctacaaact
     5761 ggcggatctg acggttggtc tgcgtgtacc ggaagagcag gagcgagaag ggctggatgt
     5821 caacagtcac ggcgagaatg cctataacgc gtaacaagca ctgcaaaaaa tagccggacg
     5881 gttttacctc cggctatttt tttaattgtg attacgcatc accccttcct gaacggtcga
     5941 ggcaaccagc acgccgtcct gggtataaaa ctcaccgcgc acaaagccgc gtgcgctgga
     6001 cgccgaggtg ctctccacgc tatacagcag ccattcattc aaattaaacg ggcgatggaa
     6061 ccacatggaa tggtcaatgg tggcaatctg aatccccggt tcgagaaaac cgatgccgtg
     6121 cggctgtaga gctaccggca ggaagttaag atcagaagcg taaccgagca gatactgatg
     6181 aacgcgcagg tcatccggca cgctaccatt tgcgcggatc cacacctgac gatgtggttc
     6241 tgcgacgtga cctttcagtg ggttatgaaa ctccaccgga cggacttcca gcggacgatc
     6301 gcagatgaat ttatctttaa gcactggcgg cagcaggtgc gccagcgatt gggcgatttg
     6361 cgtttccgaa ggcaggccat caggcgctgg cgcggacggc attgtttttt gatgttcgaa
     6421 acccgcttct ggtgcctgga aagaggccgt catataaaaa atcggtttgc cgttttgaat
     6481 agcagcaacc cggcgggcgc tgaagctgtt accgtcacgc agcgtttcga catcataaat
     6541 aatcggcttc ttactatcgc cagggcgaag aaagtagctg tgaaacgaat gtaccagccg
     6601 ctcttcaggg acggtctctt ttgcagcata caaggcctga cccacgacct ggccgccaaa
     6661 cacctggcgt aaacctaaat cttcactctg gccgcgaaag agtccttcct caattttttc
     6721 cagatttaac aatgtcagta aattttttag cgcctgactc atataactct ccagtaacaa
     6781 agctgccgca gcaagccaaa gtgagttgag tataacgcaa atttgctact ggtccgatgg
     6841 gtgcaatggt ctgaattacg ggctaattac aggcagaaat gcgtgatgtg tgccacactt
     6901 gttgacgtta ctattttgtt aaccactctt ccggcgagga aagttagccc gctgatgcat
     6961 tgataataag gagaaatgaa tgaaactcgt gcacatggcc agtggtttag cggttgcgat
     7021 tgcgttggcg gcttgcgcag ataaaagcgc ggatattcag acgccagccc cggctgcaaa
     7081 tacgtctatt tcagcaacac aacaacctgc tatccagcaa ccgaatgtct ccggtaccgt
     7141 ctggatccgt cagaaagtcg cactgccgcc tgatgctgtg ctgaccgtga cactttctga
     7201 cgcgtcgtta gccgatgcac cgtcaaaagt gctggcgcag aaagcggtgc gtactgaagg
     7261 taaacagtca ccattcagct ttgttctgcc atttaacccg gcagatgttc agccgaacgc
     7321 gcgtattctg ttgagtgcgg cgattaccgt gaatgacaaa ctggtattta ttaccgatac
     7381 cgttcagccg gtgatcaaca agggcggaac taaagccgac ctgacattgg tgccggtgca
     7441 gcaaaccgcc gtgccggttc aggccagcgg tggtgcaacg actaccgtac cttcgacttc
     7501 accaactcag gtgaatccgt cttcggcagt tccagctcct acgcaatatt aagcgacgtt
     7561 aaccctctcc gcccggagag ggttagtagt tccagcgata acgctgcaag tcgatttgcc
     7621 cgcttcccga taccatcaca ccttctgcca gtaatgcctg tcgctgacgc tgtaaatccg
     7681 gtccggttag cgaaattgtg ccgtggcgat taaccacccg gtgccagggt aaggtgctgc
     7741 cttcagggag acgctttaac acaccgccca cctggcgcgc ggcgcggggc gatcccgcca
     7801 gtttcgccac atcaccgtaa gtggtgacat agccttcggg aatagcggcg acgatttgcc
     7861 agacgcgttg gggaaatgaa tcttcttttt ccatcttttc ttcctgaggt aatttttcag
     7921 cataatctgg aaaaacgccc gagtgaagtc gcattgcgca agaaaccagc atctggcacg
     7981 cgatgggttg caattagccg gggcagcagt gataatgcgc ctgcgcgttg gttctcaacg
     8041 ctctcaatgg gggctctgtt ggttctcccg caacgctact ctgtttacca ggtcaggtcc
     8101 ggaaggaagc agccaaggca gatgacgcgt gtgccgggat gtagctggca gggcccccac
     8161 ccatttctgc ctcccaccgt ttcgtcaaaa aaccccaaca tggctaaact ttaaccacaa
     8221 ctgacgtcgc aagaattgtc tggctgcgca gtacgcttcg gaggtatgtc tgatgaagta
     8281 tgttgatggt tttgtggttg ccgttcctgc cgataaaaag gatgcctatc gggaaatggc
     8341 cgctaaggct gcgccattgt ttaaagagtt tggcgcgctt cgtattgtcg aatgctgggc
     8401 cagcgatgta ccggatggca aagtgaccga ttttcgtatg gcggtgaaag cggaagagaa
     8461 tgaagaggtt gtctttagct ggattgaata cccttcaaaa gaggtccgcg acgctgctac
     8521 tcaaaagatg atgtcggacc cacggatgaa agagttcggc gagtccatgc cgtttgacgg
     8581 caagcgaatg atctatggcg gattcgagtc aatcatcgac gaataggtag cgtgacgggc
     8641 tgtgcggcgt gccgtcagcc caaaaattca caaatgttgc tcggcccagc gtaagaaatc
     8701 ttctttcggt aatgccttgc tgtagagcca gccctgaccg tagtgcacgc catgctggcg
     8761 tagccactct tcttgtttac tggtttcgat tccctccgct accattttca gtttcagtgt
     8821 ttttgccatt tcgatgatat gcggcgtgac atttttatat tccagcgcat caacgaaaga
     8881 tttatcgatc ttcagaatgt cgacatccag atcctgtaaa taacttaaac ttgaatatcc
     8941 cgtaccaaaa tcatcaagat aaatttcatg gcccgcctcc cggtagcgag aaattatcgg
     9001 ggcgctggtt ttcggatcgg caaactcgcg ttcagtgagt tcaagcgcga tctgtctggg
     9061 attaacctga tagtgattga tcatttcacg cagcaattgc gggatttttt ccgaggtgag
     9121 cacggtggat tcaagattga tcgaaatatg ctgctgtgga tgctgacgca gccagtcgcc
     9181 catatcttca aagacgcttc ttataatcag tagcgtcaat ggctcagaaa ggcccgtttg
     9241 ctgtgccagc ggaataaaac tatctggtga caaccaacta ccgtctgtct gcggccagcg
     9301 cgccagtgcc tcagcaccga caattttgcc attggctaag gagacaatcg gctgatagtg
     9361 cacgcaaata tcacgatttt cgatagcatc ctgcagccga tgatgcggtg actggatacg
     9421 gcgcaaaata cgcagcacaa acatcgctgc cagcaggcca atcaccaacc cgagcggtaa
     9481 ccaaataaag acttgccgat gccagccttt ctgcaacatt ttcgttgaag cccaggtgat
     9541 gatcgaaata ttcatctccg ggaagggcag gatatcgtaa atgattccat tattttcgat
     9601 atgctcaccg ggtgtttttt gtagcctggt aataattccc tgagcaattt catcgctgct
     9661 ggttatgaca acgttatggg cattgccaat aatggcggca tcaatttgcc atgagctata
     9721 gggaatgaca tcaataaagg aagcggggtc gatcatgacg acataatgtg ccgttcccat
     9781 ggcgaccatg taacggataa tgcctaaatc gttatgcgat gttaaccaga cacgataacc
     9841 atctttcgaa attttacctg gctcggggaa ggtatcgggc ggactttcat gctccagaga
     9901 cgaacactgg ggaacgttgt tatcgatata aaccacttcc tgaatatagc gataactgta
     9961 agatacccga cgcatttcca tgagatgggc ttcgctacag gcagcgcctt gccatctttc
    10021 cagctcttgc agcgcatctt tcccttgtgt cgccacctta ttggcccgaa tagcgacgcg
    10081 ggaggaatag gtatccagct cttcaataaa cgatgtttct acctgctgat gggccagcca
    10141 gatgcttaag ccgacaggca gcaatactga aagaatcagt actcccgaaa taaggccgac
    10201 cagatgtcgt gttctcacca gcatatcctt attaacacag tgtagggaaa gtatatctga
    10261 ttgtggaaaa aggctggcag gagataagga ggtagagctg gtatttcatg aagtgatggg
    10321 tggaaacgat cattgtcatt tgaccaggcg cagagcttta cgcctggtat gtaaccctaa
    10381 cttaagatgt tgattcggcg cgagcgaggg taaaaatatc gtaaaaagac agttcacctt
    10441 tacggacgat cattttttgc agttgttcct tatgaacatc cgcgacacgc ggagagttca
    10501 agatggcttc aaccagcgcg gcaggtacaa agcgcgtgtt gtaccattcg ccgttatagc
    10561 aaacgcggaa atccagcacg tcgatatctt catagcggta gcgtgggtag acatcgaaaa
    10621 agaagaaacc attaagcagg ataaataata caaccagtag ccagacagag cctgacagcg
    10681 tttcggactg caacatcacc gccagggtgg caaaaaaagc aacgtacata ccgataaaca
    10741 gccccggatg tttacggata aaactgatac taaagcgggg tttgttgtcg cgtttttcgc
    10801 gggtattcag agactcaatc gtttcggtca gcaggcgttg tatttcggtc atcgtttgcc
    10861 ttcgtggtat ctgcaacttt ccagggaaca ccctatttta acgtgggaat agtagagggg
    10921 gagtaacaga tttaccacaa ttttgcataa cagttgcgaa aaaccgatta caatttttta
    10981 attattctgg ctggattacc gcccacgaca acgttgtccg ggacatcttt tgtgacaact
    11041 gcacctgagg ctaccacgac gttatcacca atggtcacac cagggttaat gaccgcgcgt
    11101 ccgccaatcc agacgttatt accgatggtg acgggtttcc ccagttcagc accgctatta
    11161 cgtgctacag ggtcgatggg atgtgttgcc gtgtagatat gaacgcctgg tgccaacata
    11221 cagttatcac cgatgcgaat agggcagaca tcaagcatca cgcaatcgaa gttggcgaaa
    11281 aaattattac cgagaaaaat gttatagcca tagtcacagc gaaacgttgg ctcaatataa
    11341 gcctctgtca cctgaccgaa tagatcagcg agaatttgct ggcgtaatgt gtgctcttcc
    11401 gccagggaat gattgtatcg gtgaataagc tgacgagcgc gcaggcgatc gcgagataac
    11461 gtctcatctg ccgagcgata caactcacca gcaatcatct tttctttttc tgtgctcatt
    11521 actgacctcc ggttgctgaa aatgcggcaa cacagtaaga cgaaaagtca gtgaagagaa
    11581 cgttcccatt acatctgtgt gataaagatc acatagggta tgtcttcatg gtgaaaaagt
    11641 ataaaattct taataaacag ccggttatag ctccgaaagc gaattattta ttagcgaata
    11701 aatttccata ctgaggaagg gatcttgtcg tatagtttat tcatggtcaa ttcggcgagg
    11761 cggtgatctg cggctgagta aaataccgcc agttcattat ctgataattc gtatttattt
    11821 ttctcgataa cacgctccag cgtgtcaatt gtctggcaac gacgtaaacg cattaaataa
    11881 tcggttttcg ttaaaggttt ttcggacata cttctaccca tggttgtaat aattctaaca
    11941 agataaactc gcaggattct ctttcgtcgc attgacaaaa caacggaata gtcgattacc
    12001 tgacttccgc catttctgaa gatcctgcat attaatacca taactgctga acaacataaa
    12061 ggtgtcatcc agatattcgt cgatctgctc aatgagctta ttgtcttcat tatacttaat
    12121 tttgtaatta agtgcgaagg tcgcaatatg ctcaatcagt tcattcaact ggaggttgat
    12181 cgccgaggtt gggtcgttaa cccagccatg attgctttct tcaaggtttg caagacagtc
    12241 atgatacagg gtttcacaga gaaacttaag ctgtgcgata tcatgtcttt tgggtgagta
    12301 ttcatccata acgcgtcccc ttcttagcgg ttgaactaac ggacaccttt cgggatggaa
    12361 aaaacttact gacctggact tgccctctcg tttgttagct taactataag ccactctttg
    12421 caggttttca tcgcattatt acgaaaaatt acaattagga aggaagcggg acaggactca
    12481 ctttttcata ctagcactaa cccgcagcag gtaaaagcag tttgctcgaa ctactcataa
    12541 catttcagga ttatcgccag atgaaattgg tgacactaat accaggattg ctctgaatat
    12601 gacgtaataa ccgaggaatg aataaagaat taccgcaaat aattaagaat agcctctaaa
    12661 tgattatgga ttattaaatc tattagcgct acttaatata aattaactaa caatcagtat
    12721 tttttatgaa ttttatacgt ggttaatact ggttttcgta tgagatcctg agttggtggt
    12781 tcaattactc cttaatgttc gtaggttatg cataaaaaag gccgcttacg cggccttagt
    12841 gattacatgt tgtatcaatg atgatcgaca gtatggctgt gctcgatatc ttcattcttg
    12901 cggctaaagc ggcggcgaac caccacaaag aataccggaa cgaagaagat tgccagtacc
    12961 gttgcggtca ccatcccgcc cattacaccg gtacctactg cgttctgcgc gccggaacca
    13021 gcaccagtac tgataaccag cggcataacg ccgaggataa acgccagcga ggtcatcagg
    13081 atcggacgta aacgcatccg caccgcatca agcgtcgctt caatcagacc tttaccttct
    13141 ttatccatca agtctttggc gaattcgacg ataagtatcg cgttcttcgc cgacaaccca
    13201 atggttgtga gcaggcctac ctggaagtaa acgtcattgg tcaggccacg gaaggtggca
    13261 gccagcaacg caccgataac ccccagcgga acgaccagca taacggagaa cggaatcgac
    13321 cagctctcgt acagtgctgc cagacacagg aacacgacaa tcaacgaaat cgcgtacagt
    13381 gaaggtgcct ggttgccgga gagacgttcc tgataggaca tccccgtcca gtcatagccc
    13441 acaccggtag gcagtttgct cgccagttgt tccattagct ccattgcttc accggtactt
    13501 ttacccggtg ccgcctggcc taagatttcc atagatggca ggccgttgta acgttccaga
    13561 cgcggcgaac cgtactccca acgagaagag gagaacgccg agaacggcac catctgacca
    13621 tcagcagcac gaacatacca gtcgccgata tcatccggca gcatacggta tttcgcttct
    13681 gacatgacgt aaactttctt cacacgaccg cggtcgataa agtcgttcac atagctacca
    13741 ccccatgcag cgcccagagt ggtgttaatg tcgttgatag aaacacccag cgcctgcgct
    13801 ttttcctggt cgatatcaat cttaaactgc ggggtatctt ccagaccgtt tggacgtacg
    13861 ctggtcaaca tatcagggtg cttcgctgct tctgcaagca gctggttacg cgcctgagtc
    13921 agtttttcgt gaccaaggcc agcctggtca atcagctcaa agtcaaagcc ggttgcagta
    13981 cccagttcca cgattgcggg caggttaaag gcgaaaacca tcgcatcttt gatttgcgag
    14041 aaagcgcgtg ttgcacgcat agtaatcgct tcaactttgt tttcttcgcc cggacgatcg
    14101 gcccagtcct tcaaggaaac gaacgcaata ccggtattct gaccacgtcc cgcaaagccg
    14161 aagccgttaa cggcgaacac cgactcaacg ttgttctttt ctttggtcag atagtaatgc
    14221 gttacctcat tgagcacttt ctgcgtacgt tcctgcgttg cacctgctgg cagctgaacc
    14281 atggtcataa atacgccctg gtcctcatct ggcaagaagg agcttggcag acgcacgaac
    14341 agataggcca tgccgaccac gatgatcaga tacagcacca ggtaacgccc cgtactgcgc
    14401 agaataccgc ctacgctgtc ggtgtagtgg tgcgtgctct tctcgaacat gcggttaaac
    14461 cagccgaaga agcctttttt accttccccg tgatcgcctt tggcaatcgg tttcagcatg
    14521 gtggcacaaa gagccggagt caggatcaac gctaccagta ccgacagcgc cattgctgaa
    14581 acaatggtaa tagagaactg acgatagata gcaccagtag aaccgccaaa gaaggccatc
    14641 ggtacgaata ccgccgacag taccatcgcg ataccgacca gagcgccctg aatctgcccc
    14701 atcgacttac gggtagcttc ttttggcggc aaaccttctt ccgccataac acgctcaacg
    14761 ttttctacca caacgatagc gtcatccacc aacaagccga tggcgagcac catcccgaac
    14821 attgttagcg tgtttatcga gaagccaaag gcggcaagga cggcaaaggt cccgagcaat
    14881 accaccggta cggcaatggt cggaatcaac gtcgcgcgga agttctgcag gaacagatac
    14941 atcaccagga acacgaggat gatcgcttcg accagcgttt taaccacttc gtgaatagag
    15001 attttcacga acggcgtggt gtcgtacggg taaacaattt tcagacctga cgggaagaac
    15061 ggttccatct tcgccagttc agcacggatt gccgcagcgg tatccagcgc gtttgcaccg
    15121 gtcgccagct tgatccccag accggaagcc ggttggccgt taaactctgc gatgatgtcg
    15181 tagttctcac cacccagctc aatcttcgcc acatcacgca gcagtacgcg ggaaccatcc
    15241 tgattcactt tcagcaggat tttgccgaac tcttcagtag aggtcagacg cgtctgagca
    15301 ataatagagg cgttaagctg ttggcctttc accggcggcg taccaccgag ctgacccgcc
    15361 gcaacctggg cgttctgcgc tttgatggcg gtaatgacat caaccggcgt tagctggaat
    15421 ttgttcagct cattcgggtt catccagata cgcatcgcgt actgtgaacc gaacaactga
    15481 acatcaccca cgcccgacgt acggctgatg gcatctttca tattcgccgc tacgtagtca
    15541 gagatatcct cctgcgtcat ggtgccatcg gtgttgataa cgccgacaac catcaggaag
    15601 ctgctggatg atttctcaac gctcacccct tgctgctgaa cttcttgcgg cagcaacggc
    15661 atcgccagct gcagtttgtt ctgcacctga acctgcgcga tatccgcatc agtaccagac
    15721 tcaaaggtca gggtgatctg cacggtaccc gtggagtcac tgttagagga catgtacatc
    15781 aggttatcga taccgttcat attctgttcg ataacctgtg tcaccgtgtc ctgcactgtt
    15841 ttcgcatcag cgccggggta ggaggcggag atcgttactg ccggcggtgc aatcgtagga
    15901 tattgcgcca ccggcagttt gaggatcgcc agcccccctg ccaacatgat gataatggcg
    15961 atcacccacg caaaaatcgg gcgatcgata aagaaattag gcatgtctta acggctcctg
    16021 tttaagttaa gacttggact gttcaggctg agcaccgctt gcggcttgct ggttattatc
    16081 agcggtaact tcttgtgctt ttacctggac accaggacgc actttctgca gcccacttat
    16141 tactacgcga tcgcctgctt tcagaccttc tgtcaccagc cacttatcgc caatagcctg
    16201 gcttgcaacg atcggacggg tctccacttt gtcatccgcg ccaactacca gtacggtggc
    16261 atcgccacgc ggcgtacggg ttacgccctg ttgcgggact aaaatagcgt ttggattaag
    16321 cccttcttca agacgtgcgc gcacgaacat acccggcagc agagtgtgat ccgggttcgg
    16381 gaagatagcg cgtagggtga tagacccagt ggtctgatca acggtaacgt cagagaattc
    16441 cagcgtaccg tcctgcggga acttaatgcc gtcactggtg atcagcgaca ctttggcttt
    16501 gccgttctct tgtttcagcg tgccattcgc cagttcctgt ttcaggcgca ggaagtcgtt
    16561 gctggactgg gtcacatcaa cgtagatcgg atcaagttgc tgcacggttg ccagcgcagt
    16621 cgcctgaccg ttctgtacca atgcgccttc cgtcacgttc gacttaccaa tgcgaccgct
    16681 aatcggagag gtgactttgg tgtaagccag attgatccgc gcagtttcaa cggcagcttt
    16741 cgccgcagtt accgcagcat tcgcctgttg cgcatcagcc agagcctgat cgtactcttg
    16801 cttactgatg tactgagtac cgagcagttt ctgataacga ttcaccgtca attgcgcgat
    16861 attggctgca gcctgggctt tcgccagatc acctttcgca ctgtcgtatg tcgcctgata
    16921 ggtcgcagga tcaatctgat agagagagac acctgcttcg atgtcgctac cttctttgaa
    16981 attacgcttc aggataatcc cgctaacttg aggacgaact tctgcgatcc ggtaggcact
    17041 ggtgcgaccc ggaagctcgg ttgtgatctg cagaggttca gttttgactg ttactacgcc
    17101 aacggcgggc atctgctggc caccttgttg ggcctgtttg tcgtcacatc ctgttagggc
    17161 taagctgcct gagagcatca gaacgaccgc cagaggcgta aaccctctgt ttttgttcat
    17221 atgtaaacct cgagtgtccg atttcaaatt ggtcaatggt caaaagttaa taaacccatt
    17281 gctgcgttta tattatcgtc gtgctatggt acatacattc acaaatgtat gtaaatctaa
    17341 cgcctgtaaa ttcacgaaca tatggcacga aaaaccaaac aagaagcgca agaaacgcgc
    17401 caacacatcc tcgatgtggc tctacgtctt ttctcacagc agggggtatc atccacctcg
    17461 ctgggcgaga ttgcaaaagc agctggcgtt acgcgcggtg caatctactg gcattttaaa
    17521 gacaagtcgg atttgttcag tgagatctgg gaactgtcag aatccaatat tggtgaacta
    17581 gagcttgagt atcaggcaaa attccctggc gatccactct cagtattaag agagatatta
    17641 attcatgttc ttgaatccac ggtgacagaa gaacgacgtc gattattgat ggagattata
    17701 ttccacaaat gcgaatttgt cggagaaatg gctgttgtgc aacaggcaca acgtaatctc
    17761 tgtctggaaa gttatgaccg tatagaacaa acgttaaaac attgtattga agcgaaaatg
    17821 ttgcctgcgg atttaatgac gcgtcgggca gcaattatta tgcgcggcta tatttccggc
    17881 ctgatggaaa actggctctt tgccccgcaa tcttttgatc ttaaaaaaga agcccgcgat
    17941 tacgtcgcca tcttactgga gatgtatctc ctgtgcccca cgcttcgtaa tcctgccact
    18001 aacgaataac cctgaatctg actccaggat ttttcctgga cattttcgtc gttgctattc
    18061 tggttcactg cgtcgtgata ttcttgcggt ttgacttttt caggtcgttc ttcaggttca
    18121 gaaaccttca ttcatcatga ctatgttcca gtattacaaa cgatcacggc attttgtttt
    18181 ttcagcattt attgcttttg tttttgtctt gttatgccag aacacggcgt ttgcgcgggc
    18241 gtcatcgaat ggtgatctgc cgacaaaagc ggacctgcag gcgcaacttg actcactaaa
    18301 taaacaaaaa gatctttctg ctcaggacaa actggtgcag caggatctga cagatacatt
    18361 agccaccctc gataaaatcg atcgcataaa agaagagaca gttcagctac ggcaaaaagt
    18421 cgctgaagcg ccggaaaaaa tgcgccaggc gaccgcggcg ttaacagcac ttagcgatgt
    18481 cgataacgac gaagaaacgc gcaaaattct gagtacgctt tcgttgcgcc agctggaaac
    18541 tcgcgttgcc caggcgctgg acgatttgca aaacgcacaa aacgatctgg cgtcttataa
    18601 cagccagctg gtttcgttac agacgcagcc cgaacgcgtg caaaatgcga tgtataacgc
    18661 ttcgcagcag ctgcaacaaa ttcgcagtcg tctggatggg actgatgtcg gcgagactgc
    18721 cttacgtccc agccagaaag tgttaatgca ggctcagcag gcgttgctga atgcggagat
    18781 tgaccagcag cgtaaaagcc tggaagggaa caccgtcttg caggatacct tgcaaaagca
    18841 acgtgattac gtgacggcga acagcgcccg tctggagcac cagttgcagc tgttgcaaga
    18901 agcggtaaac agcaagcgcc tgactttaac tgaaaaaacg gcgcaggaag ccgtctcccc
    18961 ggatgaagcc gcgcgtattc aggctaatcc gctggtgaaa caggaactgg aaattaacca
    19021 gcagttaagt cagcgtctga ttaccgcgac tgaaaacggt aatcagttga tgcagcaaaa
    19081 cattaaagtc aaaaactggc tggagcgggc gctgcaatcg gaacgcaata ttaaagagca
    19141 gattgccgtc ctgaagggca gcctgctgtt gtctcgtatc ctttaccagc aacaacaaac
    19201 gctgccctcg gcggatgaac tggaaaacat gaccaaccgc atcgcggatt tgcgtctcga
    19261 acagtttgaa gttaaccagc agcgtgatgc acttttccag agcgatgcgt tcgtcaacaa
    19321 actggaagaa ggtcacacca acgaagtcaa cagcgaagtt cacgatgcgt tattgcaagt
    19381 ggttgatatg cgtcgcgaat tgctggatca actcaacaaa cagttgggta accagctgat
    19441 gatggccatt aacctgcaaa tcaaccagca gcagttaatg agtgtgtcga aaaacctgaa
    19501 atccatcctg actcagcaaa tcttttgggt gaacagtaac cgtccaatgg actgggactg
    19561 gatcaaagcg ttcccgcaaa gcctgaaaga tgaatttaag tcgatgaaaa tcacggtgaa
    19621 ctgggaaaaa gcctggcctg ccgtttttat cgctttcctc gccggtttgc cgctgctgtt
    19681 gattgccggg ctgatccact ggcgtctggg ctggctgaaa gcgtatcaac agaaactggc
    19741 ttccgctgtg ggttccctgc gtaacgatag ccagctcaac acaccaaaag cgatccttat
    19801 cgacctgatc cgcgcgctgc cggtgtgcct gattattctc gcggttggcc tgattctgtt
    19861 gaccatgcag ctcaacatca gcgaactgct atggtcgttc agtaaaaaac tggcgatttt
    19921 ctggctggtg tttggcctgt gctggaaggt actggagaaa aatggcgttg ccgtgcgtca
    19981 cttcggcatg ccggagcagc agaccagcca ctggcgtcgg caaattgtcc gcatcagtct
    20041 cgcgttgctg cctatccatt tctggtctgt ggtagcagaa ctttccccgc tgcatctgat
    20101 ggatgatgtg ctggggcagg cgatgatttt cttcaacctg ctgctgattg ccttcctggt
    20161 atggccaatg tgccgcgaaa gctggcgtga taaagagtcg cacaccatgc gactggtcac
    20221 cattaccgtg ctatcgatta tcccgattgc gttgatggtg ctgactgcca cgggttattt
    20281 ctacaccacg ctgcgcctct ccgggcgttg gattgaaacc gtttatttgg tgatcatctg
    20341 gaacctgctg taccagacgg tattgcgcgg cttaagcgta gcggcgcggc gtatcgcctg
    20401 gcgtcgtgcg ctggcgcgtc ggcagaatct ggtgaaagag ggcgcagaag gtgccgaacc
    20461 gccggaagaa cccaccattg cactggagca agttaaccag cagacgctgc gtattaccat
    20521 gttgctgatg tttgcgctgt tcggtgtcat gttctgggca atttggtccg atttgatcac
    20581 cgtgttcagc tatctcgaca gcatcacgct ctggcattac aacggcactg aagctggcgc
    20641 tgcggtggtg aaaaacgtca ccatgggcag tctgttgttt gcgattatcg cctcaatggt
    20701 ggcctgggcg ttgattcgca acctgcctgg tttactggaa gtgctggtgc tctcgcgact
    20761 gaatatgcgt cagggcgcgt cgtatgccat tactaccatc cttaactaca tcattattgc
    20821 cgttggtgcg atgacggtgt tcggatcgct gggcgtctct tgggataaac tccagtggct
    20881 ggccgcagca ttatccgtag gtcttggttt tggtttacaa gaaattttcg gtaacttcgt
    20941 ctccggtttg atcattctgt tcgaacgtcc ggtgcgtatt ggcgatacgg taaccattgg
    21001 tagcttctcg gggacggtaa gtaagatccg tattcgtgcg acaacgatta ccgatttcga
    21061 tcgcaaagaa gtgatcatcc cgaacaaagc gtttgttacc gagcgtctga tcaactggtc
    21121 gttgactgac actactacgc gtctggtgat ccgtctcggc gtggcctatg gctccgatct
    21181 ggaaaaagtg cgtaaagtgt tactgaaggc ggcgactgag cacccaaggg tgatgcacga
    21241 accaatgccg gaagtcttct ttacggcgtt tggtgccagc acgttggatc atgagctgcg
    21301 tctgtatgtg cgtgaactgc gtgaccgtag tcgtactgtc gatgagctga accgtactat
    21361 cgatcagctg tgccgtgaaa acgacatcaa cattgccttt aaccagcttg aagtgcatct
    21421 gcacaacgag aagggcgatg aggtgacgga agtcaaacgc gactacaaag gcgatgaccc
    21481 gacgccagcg gtagggtaat aaccaaaggt ggcgacattt agttgccccg agattactga
    21541 caaagtgcgc gttgttcatg ccggatgcgg cgtgaccgcc ttatccggcc tacgaaaccg
    21601 caagaattca atgtattgca ggagctgt
//
LOCUS       DAZFQD010000083        21478 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.083, whole genome shotgun sequence.
ACCESSION   DAZFQD010000083 DAZFQD010000000
VERSION     DAZFQD010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21478
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.083"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..326
                     /locus_tag="ACHKTW_003142"
     CDS             <1..326
                     /locus_tag="ACHKTW_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO6464114.1"
                     /translation="ITLEQLSKKFRASVSYICHEFTKEYRISPINYVIQRRMTEAKWS
                     LTNTELSQAEISWRVGYENVDHFAKLFLRHVGCSPSDYRRQFKNCFAEQEILSEFPQP
                     VSLAG"
     gene            complement(369..1520)
                     /gene="ydiO"
                     /locus_tag="ACHKTW_003143"
     CDS             complement(369..1520)
                     /gene="ydiO"
                     /locus_tag="ACHKTW_003143"
                     /EC_number="1.3.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HFO6464115.1"
                     /translation="MDFSLTEEQELLLASIRELITTNFPEEYFRTCDQNGTYPREFMR
                     ALADNGISMLGVPEEFGGIPADYVTQMLALMEVSKCGAPAFLITNGQCIHSMRRFGSA
                     EQLRKTAESTLETGDPAYALALTEPGAGSDNNSATTTYTRKNGKVYINGQKTFITGAK
                     EYPYMLVLARDPQPKDPKKAFTLWWVDSSKPGIKINPLHKIGWHMLSTCEVYLDNVEV
                     EESDMVGEEGMGFLNVMYNFEMERLINAARSTGFAECAFEDAARYANQRIAFGKPIGH
                     NQMIQEKLALMAIKIDNMRNMVLKVAWQADQHQSLRTSAALAKLYCARTAMEVIDDAI
                     QIMGGLGYTDEARVSRFWRDVRCERIGGGTDEIMIYVAGRQILKDYQNK"
     gene            complement(1534..3129)
                     /gene="ydiF"
                     /locus_tag="ACHKTW_003144"
     CDS             complement(1534..3129)
                     /gene="ydiF"
                     /locus_tag="ACHKTW_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HFO6464116.1"
                     /translation="MKPVKPPRINGRVPVLSAQEAVNYIPDEATLCVLGAGGGILEAT
                     TLITALADKYKQTQTPRNLSIISPTGLGDRADRGISPLAQEGLVKWALCGHWGQSPRI
                     SELAEQNKIIAYNYPQGVLTQTLRAAAAHQPGIISDIGIGTFVDPRQQGGKLNEVTKE
                     DLIKLVEFDNKEYLYYKAIAPDIAFIRATTCDSEGYATFEDEVMYLDALVIAQAVHNN
                     GGIVMMQVQKMVKKATLHPKSVRIPGYLVDIVVVDPDQSQLYGGAPVNRFISGDFTLD
                     DSTKLSLPLNQRKLVARRALFEMRKGAVGNVGVGIADGIGLVAREEGCADDFILTVET
                     GPIGGITSQGIAFGANVNTRAILDMTSQFDFYHGGGLDVCYLSFAEVDQHGNVGVHKF
                     NGKIMGTGGFIDISATSKKIIFCGTLTAGSLKTEIADGKLHIVQEGRVNKFIRELPEI
                     TFSGKIALERELDVRYITERAVFTLKEDGLHLIEIAPGVDLQKDILDKMDFTPVISPE
                     LKLMDERLFIDAAMGFVLPEAAH"
     gene            complement(3273..4031)
                     /gene="aroD"
                     /locus_tag="ACHKTW_003145"
     CDS             complement(3273..4031)
                     /gene="aroD"
                     /locus_tag="ACHKTW_003145"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFO6464117.1"
                     /translation="MKTVTVKDLVIGTGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDVQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLTATLEMQEQYADRPIITMSMAKTGVISRLAGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            complement(4062..4928)
                     /gene="ydiB"
                     /locus_tag="ACHKTW_003146"
     CDS             complement(4062..4928)
                     /gene="ydiB"
                     /locus_tag="ACHKTW_003146"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HFO6464118.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGISMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTVTDLADQQAFAEALASADILTNGTKVGMKPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            complement(4940..6205)
                     /gene="ydiN"
                     /locus_tag="ACHKTW_003147"
     CDS             complement(4940..6205)
                     /gene="ydiN"
                     /locus_tag="ACHKTW_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO6464119.1"
                     /translation="MSQNKAFSTPFILAVLCIYFSYFLHGISVITLAQNMSSLAEKFS
                     TDNAGIAYLISGIGLGRLISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPNLT
                     LAYGLAVCVGIANSALDTGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSYML
                     LNNIWYGYGLIIPGILFVLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGVSS
                     VLFGVAAFSTFYVIVVWMPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLKKM
                     VRPIWANVFNSALATITAAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEFFP
                     KSKAKVTSIYMMMGGLANFVIPLITGYLSNIGLQYIIVLDFTFALLALITAIIVFIRY
                     YRVFIIPENDVRFGERKFSTRLNTIKHRG"
     gene            complement(6432..7646)
                     /gene="ydiM"
                     /locus_tag="ACHKTW_003148"
     CDS             complement(6432..7646)
                     /gene="ydiM"
                     /locus_tag="ACHKTW_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO6464120.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQTNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFGILQTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMLINALFLYRCTFPPHPGRRLPVIKKTTRSTEHRCSIIDLASYTLYGY
                     ISMATFYLVSQWLSQYGQFVAGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRPTT
                     LLMLYTFISFIALFTVCLHPTFYVVIIFAFVIGFTSAGGVVQIGLTLMAERFPYAKGK
                     ATGIYYSAGSIATFTIPLITAHLSQRSIADIMWFDTAIAAIGFLLALFIGLRSRKKTR
                     HHSLKENVAPGG"
     gene            complement(7746..8102)
                     /gene="ydiL"
                     /locus_tag="ACHKTW_003149"
     CDS             complement(7746..8102)
                     /gene="ydiL"
                     /locus_tag="ACHKTW_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HFO6464121.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTGDSESWRQWENGKCAIP
                     DRVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQRVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            complement(8238..8345)
                     /gene="rprA"
                     /locus_tag="ACHKTW_003150"
     ncRNA           complement(8238..8345)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="ACHKTW_003150"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            complement(8531..9643)
                     /gene="ydiK"
                     /locus_tag="ACHKTW_003151"
     CDS             complement(8531..9643)
                     /gene="ydiK"
                     /locus_tag="ACHKTW_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HFO6464122.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
     gene            10032..13088
                     /gene="ydiJ"
                     /locus_tag="ACHKTW_003152"
     CDS             10032..13088
                     /gene="ydiJ"
                     /locus_tag="ACHKTW_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HFO6464123.1"
                     /translation="MIPQISQAPGVVQLVLNFLQELEQQGFTGDTATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITPLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDKEMLGLNIVEFAGDDEALIDERVNALCVRLDELIASQQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLESLNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESQPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHKNSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            13085..13495
                     /gene="menI"
                     /locus_tag="ACHKTW_003153"
     CDS             13085..13495
                     /gene="menI"
                     /locus_tag="ACHKTW_003153"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HFO6464124.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGLLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGIC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            13595..13783
                     /gene="ydiH"
                     /locus_tag="ACHKTW_003154"
     CDS             13595..13783
                     /gene="ydiH"
                     /locus_tag="ACHKTW_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HFO6464125.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            14331..14699
                     /gene="sufA"
                     /locus_tag="ACHKTW_003155"
     CDS             14331..14699
                     /gene="sufA"
                     /locus_tag="ACHKTW_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HFO6464126.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQAMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFGV"
     gene            14708..16195
                     /gene="sufB"
                     /locus_tag="ACHKTW_003156"
     CDS             14708..16195
                     /gene="sufB"
                     /locus_tag="ACHKTW_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HFO6464127.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLKFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            16205..16951
                     /gene="sufC"
                     /locus_tag="ACHKTW_003157"
     CDS             16205..16951
                     /gene="sufC"
                     /locus_tag="ACHKTW_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HFO6464128.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLAVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMVFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            16926..18197
                     /gene="sufD"
                     /locus_tag="ACHKTW_003158"
     CDS             16926..18197
                     /gene="sufD"
                     /locus_tag="ACHKTW_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HFO6464129.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGEISPQQRDALALTLDAVRLVFVDGRYVSALSDATE
                     GSGYEVSINDDRQGVPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPVSHHFAHNDLLLADDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            18194..19414
                     /gene="sufS"
                     /locus_tag="ACHKTW_003159"
     CDS             18194..19414
                     /gene="sufS"
                     /locus_tag="ACHKTW_003159"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HFO6464130.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDSEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPNL
                     FDEKTRLLAITHVSNVLGTENPLAEMITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNNIAEYEQNLMHYALSQLESVPDLTLYGPRNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVD
                     RLVTGLQRIHRLLG"
     gene            19427..19843
                     /gene="sufE"
                     /locus_tag="ACHKTW_003160"
     CDS             19427..19843
                     /gene="sufE"
                     /locus_tag="ACHKTW_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HFO6464131.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRAEDRSP
                     QNSIQGCQSQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            19992..20996
                     /gene="ldtE"
                     /locus_tag="ACHKTW_003161"
     CDS             19992..20996
                     /gene="ldtE"
                     /locus_tag="ACHKTW_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HFO6464132.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVSVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(21060..21296)
                     /gene="lpp"
                     /locus_tag="ACHKTW_003162"
     CDS             complement(21060..21296)
                     /gene="lpp"
                     /locus_tag="ACHKTW_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HFO6464133.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
BASE COUNT         5587 a         5236 c         5427 g         5228 t
ORIGIN      
        1 aaatcacttt agaacagtta tcgaaaaaat ttcgtgccag cgtcagttat atttgccatg
       61 aatttaccaa agagtatcgt atttccccta ttaactatgt tattcaacgg cgtatgacgg
      121 aagcgaaatg gtcactcact aatactgaat tatcacaggc agagatctcc tggcgtgtgg
      181 gttatgaaaa tgtcgatcac tttgccaaac tgtttttgcg ccatgtcggt tgttcgccca
      241 gcgattaccg caggcaattt aaaaactgtt ttgcggaaca agaaatccta tctgaatttc
      301 ctcaaccggt aagtcttgcc gggtaaatct ggcggcgcag tttgttaaga agctgcgccg
      361 cctgcagatt atttgttctg ataatctttc aggatctgcc gacccgcaac gtaaatcata
      421 atttcgtctg taccgccgcc gatacgttca caacggacat cacgccagaa gcgggagacg
      481 cgcgcctcat cggtatagcc cagaccgccc atgatttgaa tcgcatcatc aatgacttcc
      541 attgcggtac gtgcacagta cagttttgcc agcgccgcgc tggtgcgcag tgactgatgc
      601 tgatcggctt gccatgccac tttcagcacc atgttgcgca tgttgtcgat cttaatcgcc
      661 atcagcgcca gtttttcctg gatcatctgg ttatgaccaa tgggcttacc aaaagcgata
      721 cgttggttgg catagcgggc ggcatcttca aaggcgcatt cggcaaagcc ggtgctgcgc
      781 gcggcgttga tcaggcgctc catctcaaag ttgtacatca cattgaggaa acccattcct
      841 tcttcgccca ccatgtcgct ctcttcaacc tccacgttgt cgagatagac ttcgcaggtg
      901 ctgagcatat gccagccgat tttatgcagc gggttaatct taatgccggg cttactggag
      961 tcgacccacc acagggtgaa ggcttttttg ggatctttcg gttgcggatc gcgcgccaac
     1021 accagcatat acgggtactc tttcgcaccg gtaataaagg ttttttgtcc gttgatgtag
     1081 accttgccgt ttttacgcgt gtaagtggta gtggcactgt tgttatctga gccagcacct
     1141 ggttccgtca acgccagggc ataggcggga tcacccgttt ccagggtgct ttctgccgtt
     1201 ttacgtagct gctctgcaga accgaaacgg cgcatactgt gaatacattg accgttggtg
     1261 atcaaaaatg ccggagcacc gcattttgac acttccatca gcgccagcat ttgggtgacg
     1321 taatccgcag ggataccacc aaattcttcc ggcacgccaa gcatggaaat accgttatcc
     1381 gccagcgccc gcataaactc acgcggatat gtcccgtttt gatcgcaggt gcggaaatac
     1441 tcttccggaa agttagtcgt aatcagttcg cgaatactgg ccagcagcag ttcttgttct
     1501 tcagttaaag aaaaatccat cattatactc ctgttaatga gccgcttcag gcaggacaaa
     1561 acccatcgcc gcatcgataa ataatctttc gtccatcagt ttgagttctg gcgaaatcac
     1621 tggggtgaag tccattttgt cgagaatatc tttttgtaaa tcgacgccag gggcgatttc
     1681 gattaaatgc aggccgtctt ctttcagcgt gaatacagca cgttcggtga tgtaacgaac
     1741 atccagttcc cgttcgagcg cgatttttcc gctaaaagta atttccggta gttcccgaat
     1801 aaatttattt acccgtcctt cctggacgat atgcaatttg ccatcggcaa tttctgtttt
     1861 taaactgccc gcagttaacg tgccgcaaaa aataattttc ttcgaggtgg cactgatatc
     1921 aataaatcca ccggtaccca tgattttgcc attgaattta tgcacgccga cgttaccgtg
     1981 ctggtcgact tcagcaaaac tcaaataaca aacatccaga ccaccaccgt gataaaaatc
     2041 aaactgggac gtcatatcca gaatggcgcg ggtattcacg ttcgcgccaa aggcgatacc
     2101 ctgtgaagta attccgccaa tcggacctgt ttctaccgtc agaataaagt catcagcaca
     2161 accttcttcc cgggcgacta ggccaatacc gtcagcaata ccgacgccga cattccccac
     2221 cgcgccttta cgcatttcga ataatgcacg cctcgcaact aatttacgtt ggtttagggg
     2281 caggctaagt ttggtgctgt catcaagggt gaagtcacca gaaataaagc ggttaaccgg
     2341 cgcaccgcca tacagttggc tttgatccgg atcgaccacc acaatatcca ccagataacc
     2401 cggaatacgg acagatttag gatgcagcgt ggctttctta accattttct gcacctgcat
     2461 catcacaata ccgccgttat tgtgcaccgc ctgggcaata accaatgcgt cgagatacat
     2521 cacctcatct tcaaaagtgg cgtagccttc actgtcgcag gtggtagcgc gaatgaaggc
     2581 gatatctggc gcaatcgctt tgtaatagag atattctttg ttatcaaact cgaccagttt
     2641 aatcaggtct tctttcgtga cttcattcag tttgccgcct tgctggcgtg gatcgacaaa
     2701 tgtcccgatg ccaatatcac taataatacc aggctggtgg gctgcggcgg cgcgtaaggt
     2761 ttgtgtaagt acaccttgtg gatagttata agcaataatt ttattttgtt ctgcgagttc
     2821 agaaatacgc ggcgattgtc cccagtgacc acataatgcc catttcacca gaccttcttg
     2881 cgccagagga ctaataccac ggtcggcgcg atcgccaagc cctgtcggac taataatcga
     2941 taaattacgt ggcgtttgag tctgtttata tttatcagca agagctgtaa ttaacgtggt
     3001 ggcttccaga ataccgccgc cagcgcctaa cacacaaagt gttgcttcgt cgggaatata
     3061 attcaccgct tcctgtgccg acaggaccgg cactcgtcca ttaatacgag gtggttttac
     3121 aggtttcatt gttaattcct gattcagatg tgattttccc tacgccagat ggagaattag
     3181 cgcacagaga ctcacgaaat atttttatta aatacggtta aatatatttt ttaagttcgg
     3241 cggggagggt gttcccgccg aaatattatt gcttatgcct ggtgtaaaat agttaatacc
     3301 gtgcgtaaat catttaccga gatttgccct ggtgcagagg ctttttttac cgcaccaaaa
     3361 gttgccgccg agccaaatac ttcaccagcc agacgagaaa ttacgccagt ttttgccatc
     3421 gacatcgtga tgattggacg atcggcatac tgctcctgca tctccagggt cgcggtaagc
     3481 aacgtcagca catcgctggt actttgcggc atcagcgcaa tcttaggaat atcggcgtcg
     3541 aaggactgca ttttgcgcag acgggcaatg atttcttcgg cttccggcgt tttatggaag
     3601 tcatggttgg acatgacaac tttcacatca tgcgcgtggg cgtaggcgac ggtttcttta
     3661 acctgaacat cgccggtaaa taactccaga tcgatcatat caaccaggcc gctgtcgatg
     3721 gctgcacgat tgagagcaat ataagcctcg gtggaaatcg cctgctcgcc gccttctttg
     3781 gcactgcgga aggtaaacag cagcggtttt tctggcatgg tttcacggag aatttttgcc
     3841 gccgccatga cagactccac attggagagg tcggcaaagt ggtccacacg ccattccaga
     3901 atatcaaagt ccgcttcacg ataggcgaga gcttcggatt tcacgcgggc gatatctttc
     3961 gccatcagcg agacgatgat tttaggtgcg cccgtaccaa tgacgagatc ttttacagtt
     4021 acggttttca ttttttaccc tttctgcacg cggtcagcct gtcaggcacc gaaccccatg
     4081 acctgtttaa catattccag agggaaatct ttgccagtcc ataacgtgaa ctgttcagcc
     4141 ccttgccaca acaacatgcc gtatccatca atcgttttgc aaccagcttg ttgcgcctgc
     4201 tgcaataact tcgtcatatg cgggttatac acgcattcag tgaccagcag tcccggatgt
     4261 aacagactga tatcattaac taatgattca ttctcaaggg gtttcatacc cacttttgtg
     4321 ccattggtta aaatgtcggc ggaagccagg gcttcagcaa aggcttgctg atcggcgaga
     4381 tcggtaaccg tgacgacaca atcggtgttt tcgttaaccc gctgcgcgaa ggcgagggct
     4441 ttatcgaaga actcatcccg acggttaaag agtttaattt cttttaaacc ttcaattgcc
     4501 ccctgcgcgc caatcgccgt tgaggcacct ccggccccta acagcaccat tgttttgcct
     4561 ttgatatcaa aaccgctctc tttaatggcg cgaatatggc ccgtgccgtc ggtgttatag
     4621 ccgcgcaggt agccatcatc attaacgatg gtgttgatgg cacccaccag tttggctgct
     4681 ggcgttaatt catcaacata ttcacacgcc agttgtttgt tcggcattga tataccagtt
     4741 ccgcgcattt tgagggcttt taatccttca attgctccgg gaaaactatc gttatccact
     4801 tcaaaggcca tataggtaaa tggcaatccc gctttttcta aggctttatt ctgcatttca
     4861 ggcgataaac tgtggcggat aggataggcc atcagcccaa tcaattcgta ttttgcggta
     4921 acatccataa ttaactcctt taacctctat gcttaattgt gtttaaccgg gtgctaaatt
     4981 tgcgctcgcc aaaccgcaca tcattctcag gaataataaa aacgcggtaa tagcggataa
     5041 aaacaataat tgcggtaatc agggccagca gcgcgaaagt aaaatcgaga acaatgatat
     5101 attgcaggcc gatgttcgac agataaccgg taatcagcgg aataacaaag ttagccagtc
     5161 cacccatcat catataaata ctggtgactt tggctttgct ttttgggaaa aactctgaca
     5221 tgaccgaaac accgagctgt aaaatgccgc cagctgctga gaaaccgata acaaaggcgc
     5281 cggcattaca caccagtgga gaagggtaca ggtagataat ggctgctgtt attgttgcca
     5341 gtgcagagtt aaatacatta gcccagatgg gccggaccat ttttttcagt agtgcggcaa
     5401 aaataaagac acagaccaac gagcccatac tgtaataaga gatggttttt aatgcctcag
     5461 cttctgacat accagcaaaa gccatcgcat atttgggcat ccacaccaca atcacataaa
     5521 aggtcgagaa tgcggctaca ccgaacagta ccgatgaaac accttccagc cagactaacg
     5581 gtttgctgtt catttgcggt aattcattag ctacgctggc gtccaccaac tggctgggga
     5641 atttgctttt caacagcatc agcgtgatca gtacaaacag aatacccgga ataatcagcc
     5701 catagccgta ccagatatta ttgagcaaca tatagctcac cagcattggg tagaacattt
     5761 gcccaaatga caccatcgct ttaaccagta tgaccgccga accagaggct ttcggaaagc
     5821 attccatgag cgcggggtag ccacccgtat ccagcgctga gttagcgata cctacgcaca
     5881 ctgccagacc gtaggcgaga gttaaattcg ggcaagcggg aataccaaag aagaatagca
     5941 gatacattat tactgccatt aatatcaccg cccgacgacc aaacttatcg gagatcacac
     6001 cgaagaataa aatactgatc aatcgcccca aaccgatacc ggaaattaag taggcaatgc
     6061 ccgcgttgtc agtggaaaac ttttctgcca gagatgacat attttgggca agcgtaataa
     6121 cactaatgcc gtgcaggaaa tagctgaagt aaatacaaag aacagccagg ataaatggcg
     6181 tgctgaaagc cttattttga gacattttca cagctccttg ctgaaagata atttcgttac
     6241 cgtacgtatt gttctccgac ggtttacata attactatac tgaagatgat tttccctggg
     6301 tacatggcat aaagtttatg tttccaggaa tattgcatgt catagtttgt ttttgatcgt
     6361 gattttcctt agttagcgca gaaagagtta tttatccgta agatcttgca tgacctgaaa
     6421 agaatattgc attacccacc cggagcgaca ttttccttta gcgagtgatg ccgcgttttt
     6481 ttgcggctgc gtaagccgat aaacagtgcc agtaaaaagc cgatggcagc gatggcggta
     6541 tcgaaccaca taatatcggc aatacttctt tgggacagat gagccgtaat caacggaata
     6601 gtaaaggtcg caatactgcc cgcactgtaa tagatccctg tagctttacc tttagcgtag
     6661 gggaaacgtt cagccattaa cgtcaggcca atttgcacaa cacctccagc agaggtaaaa
     6721 ccaatgacaa aagcaaatat tatcaccaca taaaatgtgg gatgcaggca gacggtaaac
     6781 agagcaataa atgagataaa ggtgtacagc atcagtaatg ttgttgggcg aacggtattt
     6841 cgaatgagtg gagcggtaat aaatacacaa agcagcgaac ccacggtgta gatactgagt
     6901 agtttgatcg acatagtgta tgacatgcct gcaacaaatt gtccgtactg tgacagccac
     6961 tggctaacca gataaaacgt tgccattgag atatagccat ataaggtata actggctaaa
     7021 tcgataattg aacagcgatg ttccgtagag cgggtggttt tctttatgac aggtaagcga
     7081 cgacccggat ggggtgggaa cgtacaacgg tataaaaaca gagcgttaat aagcataatg
     7141 cctgcagcaa tcataaagga ccaaccgaac cacagttcag cccacactaa caggctaatg
     7201 attagcggta ataaaaattg tccgctggaa acaaatgctt taattaaaat attggctgtc
     7261 ccaggtgagc gtggaaaagc ttccatcaaa ctgggataag tgcctgcatc gagaaaactg
     7321 tttgccattc ctgccagaaa gccaaaaaca taagcgataa tgatgttatt ggtctgcagg
     7381 atgccaaaaa agaaggccat atagcaacac atcccgagca tgataaaagg gcggcgacca
     7441 aagcgatcgg ataataatcc tgcaaaaagc aagacactta atcgaccaat gcccagcgat
     7501 gagataacta tcgagacacc cgcggcatta gtctgccaaa gtgtctccag cgaggccata
     7561 ttcaggctca tcaaaatgac gcccatacca tgcaccaggt aattaaaata caacccaagt
     7621 gcggtagggt aatagggatt tttcataggt tctacttagc aaaggtgctt taaaaagtgt
     7681 agcgatgttg tgtcagtatc cattcattat tgttgtaaca acgacaattc ctttctgtgg
     7741 aaaaattagc aaagccgctc cagatcgtgt gcatataatt cagcggctac cgattgatag
     7801 atcttccagt caataaaatt gccatcaggg tagactcgtt gaaatgcggt taagtcgggg
     7861 aaaaaacgca tggtgttgtt gccgatgcgg ttatttattt tctctatgat ggcatgaaga
     7921 tgtttttttc tttgctgacg catagccaac agttgctcga ctacacgatc aggaatggca
     7981 cacttgccat tttcccattg acgccagctt tcactgtcac cggtctgggc aatccaggtc
     8041 gcgcattcat caatagtcat ggcaaaaata tggcggagtg cctggagttc ataagcgttc
     8101 atcgtgtaat ggctgcatga aataaacaat aaaatataaa taacattatc agcctgctga
     8161 cggcttgaag agagtcacag tatcttgtgc aacattattg gaaagagtga ggggcgaggt
     8221 agcgaagcgg aaaaatgtta aaaaaaagcc catcgtggga gatgggcaaa gactacacac
     8281 agcaattcgt tgtttcactc aggggatttc catgcttata aatcaatatg ttgatttata
     8341 accgtgagct aatagtaggc atacggactt tttgcgtcga tcagattcgt ctcattagtt
     8401 taaaaactgt aaagaatttg cgacaggtac taccagctga atggcggtaa gcaggaaaat
     8461 atggggaaga gcaagcaaat atcaattagc ggtggttaac aatcaggcgg cagagccgcc
     8521 tgatgagaaa ttacttattc ggtttctcga tttcgccaag ttcttcgaga atttcttccg
     8581 gttggtccgt cggcggcggg acttcttcca cccacgcggc aaacagacgc caggaaacgg
     8641 ctaacagaac cggaccaata aacagaccga tcatcccgaa agcaatcaaa ccaccaataa
     8701 cgccagagag gatcaggatc agcggtaaat cggcacccat gcgaattaac attgggcgga
     8761 tgacgttatc cagcgtgcca accacaccgc tccacactaa caataccgtt ccccaggtgg
     8821 tatcgccggt ccagtagagc cagataatcg ccggaatcag taccggcaac gggccaagct
     8881 ggacaaggca ggagaggatc attaacaccg ttagcaacgt tgcataaggt acgccggata
     8941 cggcgaggcc gataccgcca agcactgcct gtactaacgc cgtcaccacc acacccagcg
     9001 ccaccgcgcg gatagcctgt gccgccagca gcacggcggc atctccgcga acgcctgcca
     9061 gacgggttgc aaaatggcga atgccttgtg ccacctgttc accgcgccag tacagcaggg
     9121 cactgaagag aagcatcagc gcacaatgca ccataaagcg cccgatatgc gccgcctgcc
     9181 caacgaacca ggtggtggtg gtgccaatat aagggcggac tttcgccatg atcgccgtcc
     9241 cccccatatc cagcaagttg tgccagcctg catacagctt cgcgccaatc accggaatgg
     9301 tattaagcca cgccagatcg ggtaacgtca tgtcgccgct ggaaatggct ttaattagcg
     9361 ggccgctgcc gtcgacgata ctgttaacca gcaaagcaat agggatgata aacaccatca
     9421 ctaataacag cgtcatcacc agaacggcga gagagcggcg gccaaacatg atcttttgca
     9481 aacgtaacaa taccggccag gtggcgataa ccaccgtacc ggcccatgca aagccgagaa
     9541 taaagggttg aacaatccac agacatgcca caatcatgat ggctaaaaac agcaccgaaa
     9601 gcagaatttg tgcgacatcc ctgggctgac gaacatttac cataactact tttcaccttt
     9661 tttgtgcgtc aaatcgtcga cgcgacgtga acacgcgaaa ctcacctgca taatgatgag
     9721 caatttcagc ggtttttaac aggccgattc tgcccttaat tctgatgggc gtgttagaaa
     9781 aaaatgtggt acaacaactc aggcaacacg caaacgattt actcgtcgta tttcaagttg
     9841 catgtgctgc ggctacgttc gttcacccca gtcacttact attgtaagct cctggagttc
     9901 actcgcttgc cgccttcctg caactcgaaa tccatagagt aaataaacat cacctttcag
     9961 tgcgctattg cagccgcctg aaagaatagt gtatcgacct acatcacaga ccgcaggaaa
    10021 gggtcaatat aatgattcca cagatttccc aggcacccgg cgtcgttcaa ctggtgctta
    10081 attttttgca agagctggag caacaaggtt ttaccggcga tacggcgaca agttatgccg
    10141 atcgtctgac aatgtcgacc gacaacagta tttaccaact tctccccgat gcggtggtat
    10201 ttccgcgttc aaccgcagat gtggcgctga tcgcccgtct tgccgcgcag gaacgctatt
    10261 catcgctgat ctttaccccc cgcggcggcg gcaccggcac taacggtcag gcgctcaacc
    10321 aggggattat tgttgatatg tcccgccata tgaaccgcat catcgaaatt aaccctgaag
    10381 agggctgggt gcgcgttgag gccggggtga taaaagatca actcaatcag tacctgaaac
    10441 cgttcggcta cttttttgcg ccggaacttt cgaccagcaa ccgggcaacg ctcggtggga
    10501 tgatcaatac cgatgcatcc ggtcagggat cgctggtcta tggcaaaacg tcggatcacg
    10561 tacttggcgt acgcgcggtg ttgttggggg gcgatattct cgatacgcaa cctttacccg
    10621 tcgaactggc ggaaacgctg ggtaaatcca ataccacaat cgggcgaatt tataacacgg
    10681 tttatcaacg ttgccgtcag caacgccagt taatcatcga caacttcccc aaacttaacc
    10741 gctttcttac cggttacgat ctgcgtcatg tctttaacga tgagatgacc gagttcgacc
    10801 tgacgcgcat tctgacgggt tcagaaggga cgctggcctt tattaccgaa gcgcggctgg
    10861 atattacgcc cttgcctaaa gtgcgccgtc tggtgaacgt caaatatgac tcttttgact
    10921 ccgcgctgcg taacgcgccg tttatggttg aggcgcgggc gctttcggta gagacggtgg
    10981 actcaaaagt gctgaatctg gcgcgggaag atattgtctg gcattccgtc agcgagttga
    11041 ttaccgatgt gcctgacaaa gagatgctcg ggctgaacat tgtggagttt gctggtgatg
    11101 atgaggcgct gattgacgag cgagttaatg ctctctgtgt gcggcttgat gagctgattg
    11161 ccagccaaca ggcaggtgtg attggctggc aggtgtgccg cgagctggcg ggtgttgaac
    11221 gtatctatgc gatgcgtaaa aaagccgttg gtctgcttgg caatgccaaa ggtgccgcta
    11281 agccaattcc gtttgctgag gatacctgcg taccgccgga acatctggcc gattatattg
    11341 ctgaatttcg cgcgctgctc gacagccacg gcttaagcta cggtatgttc ggtcacgtcg
    11401 acgcaggtgt cttgcacgtc cgtccagcgc tggatatgtg cgatcctcaa caagagattt
    11461 tgatgaagca aatctctgat gacgtagtgg cgctgactgc gaaatacggt ggtttgttgt
    11521 ggggcgagca cggcaaaggt tttcgcgctg aatacagccc ggcgtttttc ggtgaggaac
    11581 tttttgcaga actgcgcaaa gtgaaagcgg catttgaccc gcataaccga ctcaacccag
    11641 ggaagatttg cccgccagaa ggtctcgatg cgccgatgat gaaagtggac gcggtgaagc
    11701 gcggtacatt cgatcggcag atccccattg cggtacgcca gcagtggcgc ggtgcgatgg
    11761 agtgtaacgg caacggttta tgcttcaact ttgatgcccg tagtccgatg tgtccgtcga
    11821 tgaagatcac ccagaaccgg attcattcac cgaaagggcg cgcaacgctg gtgcgtgaat
    11881 ggctgcgttt gttggcggat cgcggcgttg atccactcaa actggaacaa gaactgcctg
    11941 aatccggcgt cagtttgcgg acgttaattg cccgcacgcg caatagctgg catgcgaata
    12001 aaggcgaata tgacttctca cacgaagtca aagaggcgat gtcgggctgt ctggcctgta
    12061 aagcgtgttc gacccagtgc cccatcaaaa ttgatgtgcc ggagtttcgc tctcgttttc
    12121 tgcagctcta tcacacccgt tatttacgcc cgctgcgcga ccacctcgtc gctacggtcg
    12181 agagctacgc gccgctgatg gcacgcgcgc cgaaaacctt taacttcttc attaaccagc
    12241 cgctggtgcg caaactctcg gaaaaacata tcggcatggt tgatttgccg ctgctgtcgg
    12301 tcccctcgct acaacaacaa atggtggggc atcgctcggc aaacatgacg ctggaacagc
    12361 ttgaatctct caatgcagag cagaaagcgc gcacagtgtt ggtggtgcag gaccccttta
    12421 ccagctatta cgatgcgcaa gtggtggcgg attttgtccg tctggtcgaa aaattaggtt
    12481 tccagcctgt gttactgcca ttttcgccaa atggcaaagc ccagcatatt aaaggttttc
    12541 ttaatcgttt tgcgaagacg gcgaaaaaga cggcggattt cctcaaccgt atggcgaagc
    12601 tgggtatgcc aatggtgggc gtcgatccgg cgctggtact ttgttatcgc gatgaatata
    12661 aactggcgct gggcgaggaa cgtggcgagt ttaacgtctt actggcgaat gaatggctgg
    12721 caagcgcact tgagtcacag ccagtggcta cagtcagcgg tgaatcatgg tatttctttg
    12781 gtcactgtac tgaagttacc gccttgccgg gtgcgccagc acaatgggcc gcgatatttg
    12841 cccgttttgg cgcgaaactg gaaaatgtca gcgtgggttg ctgcggcatg gcagggactt
    12901 acggacatga agcgaaaaac cataaaaatt cgctcgggat ctatgagtta tcctggcatc
    12961 aggctatgca gcgactgccg cgtaaccgct gtctggcgac cggatattcc tgccgtagtc
    13021 aggtcaaacg ggttgaaggc acgggggtac gccatcctgt gcaggcttta ctggagatta
    13081 ttaaatgata tggaaacgaa aaatcaccct ggaagcactg aatgccatgg gtgaaggaaa
    13141 catggtgggg ttactggata ttcgctttga acatattggt gatgacaccc ttgaagcgac
    13201 aatgccagta gactcgcgga caaagcagcc tttcgggttg ctgcatggag gcgcatccgt
    13261 ggtgctggcc gaaagtatcg gttccgttgc cggttattta tgtaccgaag gtgagcaaaa
    13321 agtggttggt ctggaaatca atgctaatca cgtccgctcg gcacgagaag ggcgggtgcg
    13381 cggcatatgt aaaccgttgc atctcggttc gcgtcaccag gtctggcaga ttgaaatctt
    13441 cgatgagaaa gggcgtttgt gctgttcgtc acgattgacg accgccattt tgtgacgttg
    13501 tcaccagaaa agtgtgacgc atattgcttt tggtcattat ctgcacagta aacggtggta
    13561 tactgtgcag gtcttgtcta aaagcgagga tgctatgtct actcaacttg atcccaccca
    13621 gctggccatt gaatttttac gccgtgacca gagcaatctt tcacctgccc aatatctcaa
    13681 acgtctgaaa caactagaac ttgaatttgc tgacctgctt actctctctt ctgctgaatt
    13741 aaaagaagaa atctactttg cctggcgtct gggcgtgcat tgatcctgac ttaatgccgc
    13801 aagttcgcca aatcgacctg gattaacctc cgtccaggtt cgatgacctt cttaatactg
    13861 cttatctacc gcctgatatt gttcaaaaat aatactaatc gcagtttgtg ttaaaacggc
    13921 gggttagctt tatgagatta ttcttatcgc cccttcaaga gctaagccac tgagagtgcc
    13981 ggagataagc gccggatggg gtagaaaccc ttaagcctgt gttgcacaga cttaagggtt
    14041 ttcttatttc tgaacgctgc acttttttac ctatctctta aatagtcatt ttcaatacaa
    14101 ctttttcttt ttcatcacag ccttaatgct ccttagataa taaccattat ctaacaatga
    14161 gatacctaat tcttagcgtg cctgttaacc cacacatcag ggtctatgct tattaaaagc
    14221 aggcaaaaag catgttttac ggtaaagccc ctgcgttttc tgggttgtac tgataatcat
    14281 tatcactaac atgctgttat acgctgaaag cgatgaagtg aggtaaatcg atggacatgc
    14341 attcaggaac ctttaaccca caagatttcg cctggcaagg cttaacgctg acacccgcag
    14401 cggcgataca catccgtgag ctggtggcaa agcagccggg tatggtcggc gtgcgcttag
    14461 gcgtgaagca aacgggctgc gcgggctttg gctatgtgct cgacagtgtt agcgagccgg
    14521 acaaagacga tctgctgttt gaacacgacg gcgcgaagct gtttgtcccg ctgcaagcga
    14581 tgccgtttat tgatggcacg gaagtcgatt tcgttcgtga aggacttaat cagatattca
    14641 aatttcacaa ccctaaagcc cagaatgaat gtggctgtgg cgaaagcttt ggggtatagg
    14701 cggtactatg tctcgtaata ctgaagcaac tgacgatgtc aaaacctgga ccggcggccc
    14761 gctgaattat aaagaaggat tcttcaccca gttagccacc gatgagctgg caaaggggat
    14821 aaacgaagag gtggtgcgcg caatttcggc gaagcgtaat gagccggagt ggatgctgaa
    14881 gtttcgtcta aacgcctatc gcgcatggct ggagatggaa gaaccgcact ggttgaaagc
    14941 gcactacgac aagctgaatt atcaggatta cagctactac tcagcaccat cgtgcggtaa
    15001 ttgtgacgac acttgcgcgt ctgaacctgg cgcggtgcag caaactggcg cgaacgcctt
    15061 tttaagtaaa gaggtggagg cggcgtttga gcagttgggc gttcccgtgc gggaaggcaa
    15121 agaggtggcg gtggatgcca ttttcgactc agtttcggtt gccactactt atcgcgaaaa
    15181 actggcggag cagggaatta ttttctgttc ctttggtgag gcgatccacg atcacccgga
    15241 actggtgcgt aaatatctcg gcaccgtggt gccggggaat gacaacttct ttgccgcgct
    15301 taatgcggcg gtagcctctg acggtacgtt tatttatgtg cctaaaggcg tgcgctgccc
    15361 gatggaactt tccacctatt ttcgcattaa cgcggaaaaa accgggcagt ttgagcgcac
    15421 cattctggtg gccgacgaag acagctacgt cagctacatt gaaggctgtt ccgctccggt
    15481 gcgtgacagc tatcagttac acgcggcagt ggtggaagtc atcatccata aaaacgccga
    15541 ggtgaaatat tccacggtac aaaactggtt ccctggcgat aacaacaccg gcggtattct
    15601 caacttcgtc accaagcgtg ctttgtgcga aggcgaaaac agcaaaatgt catggacgca
    15661 atcagaaacc gggtccgcga ttacgtggaa atatcccagc tgcattttgc gcggcgataa
    15721 ctccattggt gagttttact cagtggcgct gaccagcggt catcagcaag cggataccgg
    15781 caccaagatg atccacatcg gtaaaaacac caaatcgacc attatctcga aagggatctc
    15841 tgccggacat agtcagaaca gttatcgcgg cttagtgaaa atcatgccga cggcaaccaa
    15901 tgcgcgcaat ttcactcagt gcgactcaat gctgattggc gctaattgtg gggcgcatac
    15961 cttcccgtat gtcgaatgtc gcaataacag cgcacaactg gagcacgagg caacgacatc
    16021 acgtattggt gaagatcaac tgttttactg cctgcaacgc gggatcagcg aagaagacgc
    16081 catctcgatg attgttaacg gtttctgcaa agacgtgttc tcagaactgc cgctggaatt
    16141 tgccgttgaa gcacaaaaac tcctcgccat cagtcttgaa cacagcgtcg gataaggaat
    16201 aaacatgtta agtattaaag atttacacgt cagcgtggaa gataaagcta tcctgcgtgg
    16261 attaagcctc gccgttcgtc ccggcgaagt tcacgccatt atggggccaa acggttcggg
    16321 caaaagtacc ttatcggcaa cgcttgccgg gcgcgaagat tatgaagtga cgggcggcac
    16381 ggttgagttc aaaggcaaag atttgcttgc actgtcgccg gaagatcgtg caggcgaagg
    16441 catctttatg gtcttccagt atccggtgga gattccaggc gtcagtaacc agtttttcct
    16501 gcaaacggca cttaatgcag tgcgcagcta tcgcggccag gaaacgctcg accgctttga
    16561 ttttcaggat ttgatggaag agaaaatcgc tctcctgaag atgccggaag atttattaac
    16621 ccgttcggta aacgttggtt tttccggcgg cgagaaaaag cgcaacgata ttttgcaaat
    16681 ggcggtgctg gaaccggagt tatgcattct tgatgagtcg gactccgggc tggatattga
    16741 cgcattaaaa gtggtcgccg atggcgtgaa ctcgctgcgt gatggcaagc gctcattcat
    16801 cattgttacg cactaccaac gcattctcga ctacatcaag cctgattacg tccatgtgct
    16861 atatcaggga cgaattgtga aatccggcga tttcacgttg gtcaaacaac tggaggagca
    16921 gggttatggc tggcttaccg aacagcagta acgcgctgca acagtggcat cacttgtttg
    16981 aagctgaagg gacaaaacgc tcaccgcaag cacagcagca tttacaacaa ttgctgcgta
    17041 ccggactgcc gacacgtaaa catgaaaact ggaaatatac gccgctggaa gggctgacca
    17101 atagccagtt tgtcagcatt gcgggagaga tatccccaca gcagcgagat gccttagcgt
    17161 taacgttaga cgctgtgcgg ctggtatttg tcgatggacg ttacgtgtca gcactgagcg
    17221 atgcgactga aggcagcggg tatgaagtga gcattaacga cgaccgtcag ggggtacccg
    17281 acgctattca ggcggaagtg tttctgcatt tgacggaaag cctggcacaa agcgtgacgc
    17341 atatcgccgt gaagcgcggt caacggccgg caaagccatt gctgttaatg catattaccc
    17401 agggcgtggc aggtgaagag gtcaacactg cccattaccg acatcatctg gatctggcgg
    17461 aaggtgccga agcaacggtg atcgaacatt ttgtcagcct taatgatgct cgtcacttca
    17521 ccggcgcacg gttcactatc aatgtcgcag cgaacgccca cttgcagcat atcaagctgg
    17581 cgtttgaaaa cccggtcagt caccactttg cccataacga tttgttgctg gctgacgatg
    17641 ccaccgcatt tagccatagt ttcctgctgg gtggcgcagt gttacgacac aacaccagta
    17701 cgcaactcaa tggcgaaaac agcacgctgc ggatcaatag cctggcgatg ccggtgaaaa
    17761 acgaggtgtg tgatacccgt acctggctgg aacacaataa aggtttttgt aacagccgac
    17821 agttgcacaa aactatcgtc agcgacaaag gccgcgcggt atttaacggt ttgatcaacg
    17881 tcgcgcagca cgccatcaaa acggatggtc agatgaccaa caataatctg ctgatgggca
    17941 aactggcaga agtggatacg aaaccgcagc tggaaatcta tgcagatgat gtgaaatgca
    18001 gccacggcgc gacggtgggg cgtattgatg atgaacagat gttctatctg cgctcgcgcg
    18061 ggatcaatca gcaggatgcc cagcagatga tcatttacgc ctttgctgcc gaactgacgg
    18121 aagcactgcg tgatgagggg cttaaacagc aggtgctggc ccgaatcggt caacgactgc
    18181 caggaggtgc aagatgactt tttccgtcga caaagtgcgg gccgactttc cggtgctttc
    18241 gcgtgaggta aacggtttgc cgctggctta tctcgacagc gccgccagtg cgcagaaacc
    18301 aagccaggtg attgactccg aagccgagtt ttatcgtcac ggctacgcgg cggtgcatcg
    18361 cggtattcat accttaagcg cccaggcgac cgagaaaatg gagaacgtgc gcaagcgggc
    18421 atcgctgttt attaatgccc gttcggcgga agagctggtg ttcgtccgcg gcacgacgga
    18481 agggatcaat ctggtcgcca atagttgggg caacagcaac gtgcgggcgg gcgataacat
    18541 catcatcagt cagatggagc accacgctaa cattgttcct tggcagatgc tttgcgcacg
    18601 cgttggcgca gagctgcgtg tgatcccgct caatcccgac ggtacgctgc aactggagac
    18661 gctacctaat ctgttcgatg agaaaactcg cctgctggca attactcatg tctccaacgt
    18721 gcttggcaca gaaaatcccc tggcggaaat gatcacgctt gcgcatcagc atggcgcaaa
    18781 agtgctggtg gatggcgctc aggcggtgat gcatcatccg gtggacgttc aggcgctgga
    18841 ttgcgatttt tacgtattct ccgggcataa actgtatggc cccaccggaa ttggcattct
    18901 ttatgtgaaa gaagccttgt tgcaggagat gccgccgtgg gaagggggcg gttctatgat
    18961 cgccaccgtc agcctgagtg aaggcactac ctggaccaaa gcaccatggc ggtttgaagc
    19021 cggtacaccc aataccgggg gcatcattgg tcttggcgcg gcgctggagt atgtttcggc
    19081 gctggggctt aataacatag ccgagtatga acagaatctg atgcactacg cgctatcaca
    19141 gctggaatct gtaccggatc tcactctcta tggcccgcgg aacaggcttg gcgttattgc
    19201 ttttaatctt ggtaaacacc acgcctatga tgttggcagt tttctcgata actacggcat
    19261 tgctgtgcgt accggacatc actgcgctat gccattaatg gcctattaca acgtccctgc
    19321 tatgtgtcgg gcgtcgctgg ccatgtataa cacccatgaa gaagtggatc gtctggtgac
    19381 cggactgcaa cgtattcacc gtctgctggg ataacaggga ggcactatgg ctttattgcc
    19441 ggataaagaa aaattgctgc gtaatttttt acgctgcgcc aactgggaag agaaatatct
    19501 ctacattatt gagctgggcc agcgtctgcc agaattacgc gccgaagacc gaagcccaca
    19561 aaatagcatt cagggctgtc agagtcaggt gtggattgtc atgcgccaga atgcgcaggg
    19621 aattattgaa ttgcagggcg acagcgatgc ggcgattgtg aaagggctta ttgcggtcgt
    19681 ctttattctc tacgatcaga tgacgccgca ggatattgtc aatttcgatg tgcgtccgtg
    19741 gtttgaaaaa atggcgctca cccaacatct caccccatct cgttcacaag gtctggaagc
    19801 gatgattcgc gcaattcgcg ccaaagccgc tgcacttagc taaactaaag ggacagcttt
    19861 catccggttg gctcacattg ccggatggtc aatgtttaca ctgacctgca caggagtgtt
    19921 ttggtttcag gtgaacataa gcgccatccg acgtttcagc gtgagtctcc ggcaaataca
    19981 ggaggtttac aatgaaacgc gcgtctttgc ttacactcac gcttatcggc gcttttagcg
    20041 ccatccaggc tgcctgggcg gttgattatc cgctaccacc aaccggaagc cgactggttg
    20101 ggcaaaatca aacgtatacg gtgcaagaag gggataaaaa ccttcaggct attgcccgac
    20161 gttttgatac tgcggcaatg ttgatccttg aagccaataa cactatcgcc ccggtgccaa
    20221 aacctggtac gacgataact attccttcac aactgttatt acctgatgca ccgcgtcagg
    20281 ggattatcgt taaccttgca gagctgcgcc tttattatta tccgccggga gaaaatattg
    20341 tgcaggttta tccaataggt attggattgc aggggctgga aacgccggtg atggaaacgc
    20401 gtgttgggca gaaaatccct aacccaacct ggacgcctac ggcaggcatt cgtcaacgtt
    20461 cgctggagcg tggcattaaa ttaccgccag tcgttcctgc cggaccaaat aacccgctag
    20521 gacgttacgc actgcgcctc gcgcatggta atggcgaata cctcattcat ggtaccagtg
    20581 cgccggacag cgtcggtttg cgcgtcagtt cagggtgtat tcgcatgaat gcaccggata
    20641 ttaaagcctt gttctccagc gtgcggacgg gaacgccggt gaaagtgatc aacgaaccgg
    20701 tgaaatattc cgtggaacct aacgggatgc gttatgttga agtacatcga ccactatcag
    20761 cagaagaaca gcaaaacgtt cagacaatgc catacacact gccagcaggc tttacgcaat
    20821 ttaaagacaa taaggctgta gatcagaagt tagtcgataa agcgttgtat cgtcgggcag
    20881 ggtatccggt ttcggtgagc agtggagcaa ctcccgcagc cagcaatgcg ccttcagtag
    20941 agtcagcgca gaatggtgaa ccagagcaag ggaatatgtt acgcgcgacg cagtagcggt
    21001 aaacgggaga caaaaaaaat ggcgcacaat gtgcgccatt tttcacttca caggtactat
    21061 tacttgcggt atttagtagc catgttgtcc agacgctggt tagcacgagc tgcgtcatct
    21121 ttagcagcct gaacgtcgga acgcattgcg ttcacgtcgt tgctcagctg gtcaacttta
    21181 gcgttcagag tctgaacgtc agaagacagc tgatcgattt tagcgttgct ggagcaacct
    21241 gccagcagag tagaacccag gattaccgcg cccagtacca gtttagtagc tttcattatt
    21301 aataccctct agattgagtt aatctccatg tagcgttaca agtattacac aaagtttttt
    21361 atgttgagaa tatttttttg atgggaaggc acttattttt gatcgttcgc tcaaagaagc
    21421 atcgaacgct gggttttaga taaaaaaatt gagagaaaac aggatgcttc catcggat
//
LOCUS       DAZFQD010000084        21341 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.084, whole genome shotgun sequence.
ACCESSION   DAZFQD010000084 DAZFQD010000000
VERSION     DAZFQD010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21341
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.084"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1497
                     /gene="caiT"
                     /locus_tag="ACHKTW_003163"
     CDS             <1..1497
                     /gene="caiT"
                     /locus_tag="ACHKTW_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiporter"
                     /protein_id="HFO6464134.1"
                     /translation="KTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVFSYVTNV
                     WGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSAAVLFWG
                     SIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYFFFVRKM
                     EVIRPSSTLVPLVGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTECMQWLFG
                     IPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIVSGASFI
                     MNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIFLARISR
                     GRTVRELCFGMVLGLTASTWILWTVLGSNTLLLIDKNIINIPNLIEQYGVARAIIETW
                     AALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRIGWSILV
                     GIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            1528..2670
                     /gene="caiA"
                     /locus_tag="ACHKTW_003164"
     CDS             1528..2670
                     /gene="caiA"
                     /locus_tag="ACHKTW_003164"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HFO6464135.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            2799..4016
                     /gene="caiB"
                     /locus_tag="ACHKTW_003165"
     CDS             2799..4016
                     /gene="caiB"
                     /locus_tag="ACHKTW_003165"
                     /EC_number="2.8.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
                     /protein_id="HFO6464136.1"
                     /translation="MDHLPMPKFGPLAGLRVVFSGIEIAGPFAGQMFAEWGAEVIWIE
                     NVAWADTIRVQPNYPQLSRRNLHALSLNIFKDEGREAFLKLMETTDIFIEASKGPAFA
                     RRGITDEVLWQHNPKLVIAHLSGFGQYGTEEYTNLPAYNTIAQAFSGYLIQNGDVDQP
                     MPAFPYTADYFSGLTATTAALAALHKVRETGKGESIDIAMYEVMLRMGQYFMMDYFNG
                     GEMCPRMSKGKDPYYAGCGLYKCADGYIVMELVGITQIEECFKDIGLAHLLGTPEIPE
                     GTQLIHRIECPYGPLVEEKLDAWLAAHTIAEVKERFAELNIACAKVLTVPELESNPQY
                     VARESITQWQTMDGRTCKGPNIMPKFKNNPGQIWRGMPSHGMDTAAILKNIGYSENDI
                     QELVSKGLAKVED"
     gene            4090..5643
                     /gene="caiC"
                     /locus_tag="ACHKTW_003166"
     CDS             4090..5643
                     /gene="caiC"
                     /locus_tag="ACHKTW_003166"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HFO6464137.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNRYSYLELN
                     QEINRTANLFYTLGIRKGDKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESA
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYL
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQVQKYRATITECIPMMIRTL
                     MVQPPSANDRQHRLREVMFYLNLSEQEKDAFCERFGVRLLTSYGMTETIVGIIGDRPG
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYCDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            5752..6537
                     /gene="caiD"
                     /locus_tag="ACHKTW_003167"
     CDS             5752..6537
                     /gene="caiD"
                     /locus_tag="ACHKTW_003167"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HFO6464138.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPAIVNEMVM
                     TGRRMGAEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIFRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            6543..7133
                     /gene="caiE"
                     /locus_tag="ACHKTW_003168"
     CDS             6543..7133
                     /gene="caiE"
                     /locus_tag="ACHKTW_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HFO6464139.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFHGEKRQLLMGTPARAVCSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(7219..7614)
                     /gene="caiF"
                     /locus_tag="ACHKTW_003169"
     CDS             complement(7219..7614)
                     /gene="caiF"
                     /locus_tag="ACHKTW_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HFO6464140.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVAEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSRDKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(7875..11096)
                     /gene="carB"
                     /locus_tag="ACHKTW_003170"
     CDS             complement(7875..11096)
                     /gene="carB"
                     /locus_tag="ACHKTW_003170"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFO6464141.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNADFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGFETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     ERLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(11114..12262)
                     /gene="carA"
                     /locus_tag="ACHKTW_003171"
     CDS             complement(11114..12262)
                     /gene="carA"
                     /locus_tag="ACHKTW_003171"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HFO6464142.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRTDKPAFSFQGHPEASPGPHDAAPLFDHFIELIEQYRKTAK"
     gene            complement(12718..13539)
                     /gene="dapB"
                     /locus_tag="ACHKTW_003172"
     CDS             complement(12718..13539)
                     /gene="dapB"
                     /locus_tag="ACHKTW_003172"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFO6464143.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAVKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKESGLFDMRDVLDLNSL"
     gene            complement(13706..14620)
                     /gene="rihC"
                     /locus_tag="ACHKTW_003173"
     CDS             complement(13706..14620)
                     /gene="rihC"
                     /locus_tag="ACHKTW_003173"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HFO6464144.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPLGIP
                     ALLAIRDALMRAPEPVTLVAIGPLTNIALLLSQCPECKPYIRRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLSTLPQLNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVQVALDLDVKGFQQWVAEVLALAS"
     gene            complement(14686..15636)
                     /gene="ispH"
                     /locus_tag="ACHKTW_003174"
                     /gene_synonym="lytB"
     CDS             complement(14686..15636)
                     /gene="ispH"
                     /locus_tag="ACHKTW_003174"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-enyl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HFO6464145.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKRAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            complement(15638..16087)
                     /gene="fkpB"
                     /locus_tag="ACHKTW_003175"
     CDS             complement(15638..16087)
                     /gene="fkpB"
                     /locus_tag="ACHKTW_003175"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFO6464146.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            complement(16175..16669)
                     /gene="lspA"
                     /locus_tag="ACHKTW_003176"
     CDS             complement(16175..16669)
                     /gene="lspA"
                     /locus_tag="ACHKTW_003176"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFO6464147.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVILAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSK
                     AKKQ"
     gene            complement(16669..19485)
                     /gene="ileS"
                     /locus_tag="ACHKTW_003177"
     CDS             complement(16669..19485)
                     /gene="ileS"
                     /locus_tag="ACHKTW_003177"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFO6464148.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFAVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWIPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELSAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            complement(19528..20469)
                     /gene="ribF"
                     /locus_tag="ACHKTW_003178"
     CDS             complement(19528..20469)
                     /gene="ribF"
                     /locus_tag="ACHKTW_003178"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HFO6464149.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            complement(20477..20695)
                     /gene="yaaY"
                     /locus_tag="ACHKTW_003179"
     CDS             complement(20477..20695)
                     /gene="yaaY"
                     /locus_tag="ACHKTW_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HFO6464150.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGHFLPVICAMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            20798..21061
                     /gene="rpsT"
                     /locus_tag="ACHKTW_003180"
     CDS             20798..21061
                     /gene="rpsT"
                     /locus_tag="ACHKTW_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFO6464151.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
BASE COUNT         5046 a         5761 c         5513 g         5021 t
ORIGIN      
        1 aaaacgggaa tagaaccgaa ggttttcttt ccgccgttaa taatcgtcgg catactttgt
       61 tggcttacag tcagagatct ggatgcagcg aatgtcgtta ttaatgctgt attcagttac
      121 gtcaccaatg tatggggatg ggcatttgaa tggtatatgg tggtgatgct tttcggttgg
      181 ttctggctgg tgtttggccc gtatgccaaa aagcgtttag gtaacgaacc acctgaattt
      241 agtaccgcca gttggatctt tatgatgttc gcctcctgta cgtctgctgc cgtactgttc
      301 tggggatcga ttgagatcta ctactacatc tccaccccgc cgtttggctt agaaccgaac
      361 tcgacaggag cgaaagagtt ggggctggct tacagcttgt tccactgggg acctctgccg
      421 tgggccactt acagcttcct ttcagtcgcc ttcgcttact tcttctttgt ccgcaaaatg
      481 gaagtgattc gccccagctc gacactggtg ccgctggtag gtgaaaaaca cgccaaaggg
      541 ttgttcggca ctatcgtcga caacttctat ctcgtcgcct tgatcttcgc gatgggtacc
      601 agtctgggcc ttgccacgcc gctggtgacc gagtgtatgc aatggttgtt tggcattccg
      661 cataccctgc aactggacgc tatcatcatt acctgctgga ttatcctcaa cgccatttgc
      721 gtcgcttgcg gtctgcaaaa aggggtacgt atcgccagtg acgtgcgtag ttacctgagc
      781 ttcctgatgc tgggttgggt gttcattgtc agcggtgcca gcttcatcat gaactacttc
      841 accgattcgg tggggatgtt gctgatgtat ctgccgcgca tgttgttcta taccgatccc
      901 atcgctaaag gcggcttccc gcagggctgg accgtgttct actgggcatg gtgggtgatt
      961 tatgctatcc agatgagtat cttcctcgcc cgcatctccc gtggtcgtac tgtgcgtgaa
     1021 ctgtgcttcg gcatggtgct ggggctgaca gcgtcaacct ggatcctgtg gactgtactc
     1081 ggtagtaaca ctctgctgtt gatagataaa aacatcatca acattccaaa tctgatcgaa
     1141 cagtacggtg tggcgcgcgc cattatcgaa acctgggccg ctctgccact cagcaccgcc
     1201 accatgtggg gcttcttcat cctctgcttt attgccaccg ttacgctggt taacgcctgc
     1261 tcttataccc tggcgatgtc cacttgccgc gaagtacgcg atggtgaaga accaccgctg
     1321 ctggtgcgta tcggttggtc aattctggtt ggcattatcg gtattgtttt gctggcgctc
     1381 ggcggcctga aaccgattca aactgccatt atcgccggag gatgcccgct gttcttcgtc
     1441 aacattatgg tgacgctctc ctttattaaa gacgcgaaac agaactggaa agattaatta
     1501 acccccaaaa tatcaagagg ttgaaagatg gattttaatt taaatgatga gcaggaactg
     1561 tttgtcgccg gtatccgcga actgatggcc agcgaaaact gggaggccta ttttgccgag
     1621 tgcgaccgtg acagcgtcta cccggaacgt tttgtcaaag cactggcgga tatgggtatc
     1681 gacagtctgc tgatccctga agagcacggt ggtctggacg cggggtttgt tactctcgcc
     1741 gccgtgtgga tggagctggg acgtctgggg gcaccaacct atgtgctgta ccagttgccg
     1801 ggcgggttca acaccttcct gcgcgaaggc acacaagagc agatcgacaa gattatggct
     1861 ttccgcggca ccggtaagca gatgtggaac tcagcgatta ccgaaccggg cgcgggctcc
     1921 gacgtgggta gcctgaaaac gacttatacc cgtagaaatg gtaagattta tcttaatggt
     1981 agtaagtgtt ttattaccag cagcgcctac accccgtaca tcgtggtgat ggcgcgcgac
     2041 ggggcttctc cggacaaacc tgtctacacc gaatggtttg ttgatatgag caaaccgggc
     2101 atcaaagtga ccaaacttga aaagctcggt ctgcgtatgg atagctgctg tgaaatcacc
     2161 tttgacgacg tggaactgga cgagaaagac atgttcggtc gggaaggtaa cggctttaac
     2221 cgcgtcaaag aagagttcga tcatgaacgt ttcctggtag ctctcaccaa ctacggtacg
     2281 gcgatgtgcg cctttgaaga tgcggcgcgc tacgccaacc agcgcgtgca gtttggcgag
     2341 gctattggcc gtttccagtt gattcaggaa aaattcgccc acatggcgat caaattaaac
     2401 tccatgaaaa acatgctgta tgaagcagcg tggaaagcag acaacggcac catcacctct
     2461 ggcgatgcag cgatgtgcaa atacttctgc gccaatgcgg catttgaagt ggtggatagc
     2521 gcaatgcagg tgctgggcgg tgtcgggatt gcgggcaacc accgcatcag ccgcttctgg
     2581 cgtgacctgc gtgtagaccg cgtctccggg ggatctgacg aaatgcagat cctgacgctg
     2641 ggtcgcgcgg tgctgaagca ataccgctaa gtccgtgcgc tgcctgatgc gacgctaatg
     2701 cgtcttatca ggcctacggg agcacgaatg taggccggat aaagcgttta cgccgcatcc
     2761 ggcagtcatg cgctacataa atttttcagg agctaataat ggatcatcta cccatgccga
     2821 aattcgggcc gttggccgga ttgcgcgttg tcttctccgg tatcgaaatc gccgggccgt
     2881 tcgccgggca aatgttcgca gaatggggtg cggaagttat ctggatcgag aacgtcgcct
     2941 gggccgacac cattcgcgtt caaccgaact acccgcagct ctcccgccgc aatttgcacg
     3001 cgctgtcgtt aaatattttc aaagatgaag gccgcgaagc gtttctgaaa ttaatggaaa
     3061 ccaccgatat cttcatcgaa gccagtaaag gtccggcctt tgcccgtcgt ggcattaccg
     3121 atgaagtact gtggcagcac aacccgaaac tggttatcgc tcacctgtcc ggttttggtc
     3181 agtacggcac cgaggagtac accaatcttc cggcctataa cactatcgcc caggccttta
     3241 gtggttacct gattcagaac ggtgatgttg accagccaat gcctgccttc ccgtataccg
     3301 ccgattactt ttctggcctg accgccacca cggcggcgct ggcagcactg cataaagtgc
     3361 gtgaaaccgg taaaggcgaa agtatcgaca tcgccatgta tgaagtgatg ctgcgtatgg
     3421 gccagtactt catgatggat tacttcaacg gcggcgaaat gtgcccgcgc atgagcaaag
     3481 gtaaagatcc ctactacgcc ggttgcggtc tgtataaatg cgccgacggc tacatcgtga
     3541 tggaactggt gggcattacc caaattgaag agtgctttaa agatattggc ctcgcacatc
     3601 tactcggtac gccggaaatc ccggaaggca ctcagcttat ccaccgtatc gaatgccctt
     3661 acggcccact ggtagaagaa aaactcgatg cctggctggc ggcacatacc atcgcagaag
     3721 ttaaagaacg ctttgccgaa ctgaatatcg cctgtgccaa agtactgacc gtaccggaac
     3781 tggaaagcaa tccacagtat gttgcccgtg aatcaatcac tcagtggcaa acgatggatg
     3841 gtcgcacctg caaagggccg aacatcatgc cgaaattcaa aaataacccc ggacaaatct
     3901 ggcgcggaat gccctcacat ggcatggaca cggctgccat tttgaaaaat atcggctaca
     3961 gcgaaaacga cattcaggag ttggtcagca aaggtctggc caaagttgag gactaaacgc
     4021 gtggcgtcag tcggcttgcc tgactagcgc ccgcccaata acaaacgaac aaaaatggat
     4081 agaggtgcaa tggatatcat tggcggacaa catctacgtc aaatgtggga cgatcttgcg
     4141 gacgtttacg gtcataaaac ggcgctgatt tgtgaatcca gcggcggagt cgttaaccgg
     4201 tatagttatc ttgagttaaa tcaggagatt aaccgcacgg caaacctgtt ttatacgcta
     4261 gggattcgca aaggcgacaa ggttgctcta catctcgaca actgcccgga atttatcttt
     4321 tgctggttcg ggctggcaaa aattggcgcg attatggtgc cgattaacgc ccgcctgttg
     4381 cgcgaggaaa gcgcgtggat cctgcaaaat agccaggcgt gcctgctggt gaccagtgcg
     4441 caattctatc ctatgtatca acagattcag caggaagatg ccactcaatt gcggcacatt
     4501 tgcctgacag atgtggcact tcccgctgat gatggcgtga gttcgtttac tcaactgaaa
     4561 aatcaacaac ctgccacctt gtgctatgca ccgccgctat cgactgacga tacggcggaa
     4621 attctcttca cctccggcac cacctcccga ccgaaaggtg tggtgattac ccattacaac
     4681 ctgcgcttcg ctggatatta ctccgcctgg cagtgtgcac tgcgtgacga tgacgtctac
     4741 ctgacggtaa tgcctgcgtt tcatatcgat tgccagtgta ctgcggcgat ggcggcgttt
     4801 tctgccgggg ccacctttgt gctggtcgag aaatacagcg cccgcgcctt ctggggacag
     4861 gtgcagaagt accgcgccac cattaccgaa tgtattccga tgatgatccg tacgttgatg
     4921 gtgcagccgc cttcagcgaa cgatcggcaa caccgcctgc gggaagtgat gttttatctc
     4981 aacttgtcgg agcaggaaaa agatgcgttt tgtgaacgct tcggcgttcg cttgctgacg
     5041 tcttatggga tgacggaaac cattgtgggc attatcggcg atcgccctgg cgataaacga
     5101 cgctggccgt cgattggtcg ggcggggttt tgctacgaag cggagatccg cgacgatcac
     5161 aatcgcccgc tcccggcagg tgagatcggt gaaatctgta ttaaaggcgt accagggaaa
     5221 accatcttca aagagtattt tctcaacccg aaagccactg cgaaagtgct ggaagccgac
     5281 ggctggctac ataccggcga taccggatac tgcgacgaag agggcttttt ttatttcgtc
     5341 gatcgccgct gcaatatgat caaacgcggt ggcgagaatg tctcctgcgt ggagctggaa
     5401 aatatcatcg ccacccatcc aaaaattcag gatatcgtgg ttgtgggtat taaagattcg
     5461 attcgcgatg aagccatcaa agcatttgtg gtgctgaatg aaggtgaaac attgagcgaa
     5521 gaggaatttt tccgcttctg cgaacaaaat atggcgaaat ttaaagtgcc ctcttatctg
     5581 gagatcagaa aagatctgcc acgtaattgc tcggggaaaa taattagaaa gaatctgaaa
     5641 taaatgaaac agcagggaac taccctgcca gctaataatc acgctataaa acaatatgca
     5701 ttttttgcag ggatgttgtc gtccctgaaa aagcaaaaat ggagaaaagg aatgagtgaa
     5761 tcattacatc tgacccgcaa tggatcaatt ctggaaatta cccttgatcg tccaaaagcg
     5821 aatgctattg atgcaaaaac cagctttgaa atgggcgaag tatttctaaa tttccgtgac
     5881 gatccgcaat tacgtgtcgc cattattacc ggtgccggag agaagttctt ttccgcgggc
     5941 tgggatttaa aagcggcagc agaaggcgaa gcaccggatg ctgactttgg cccgggtgga
     6001 tttgcgggat taaccgaaat tttcaatctc gacaaaccgg ttatcgcagc tgtgaacggc
     6061 tatgcctttg gcggcggttt cgaactggcg ctggcggcag attttattgt ttgtgccgat
     6121 aacgccagct tcgccctgcc ggaagccaag ctgggcatcg ttcctgacag cggcggcgtg
     6181 ctgcgtctac cgaagatcct gccgcctgcc atcgtcaatg aaatggtgat gaccggcaga
     6241 cgaatgggcg cagaagaggc gctgcgttgg gggatagtca accgcgtggt tagccaggcg
     6301 gaactgatgg ataacgcccg cgaactggcg cagcagctgg ttaacagcgc cccgctggcg
     6361 attgcggcgc tgaaagagat cttccgcacc accagcgaaa tgccggtaga agaagcgtat
     6421 cgctatattc gcagcggcgt gttgaaacac tatccgtcgg ttctgcattc ggaggatgcc
     6481 attgaagggc cgctggcgtt tgccgagaag cgcgatccgg tgtggaaagg acgttaacaa
     6541 ccgtgagcta ttacgccttt gaggggttaa ttccggtggt tcacccgacg gcgtttgtcc
     6601 atcccagtgc cgtcttgatt ggcgatgtga ttgtgggagc cggtgtctac atcggcccac
     6661 tcgcctcact gcgtggtgac tacgggcggt tgatcgtgca agcgggagcc aatattcagg
     6721 atggctgcat tatgcatggc tactgcgaca ctgacaccat cgttggggaa aacggccata
     6781 tcgggcacgg agcgatcctg catggttgtg tgattggtcg cgatgcattg gtcgggatga
     6841 acagcgtgat tatggatggc gcggtcattg gcgaagagag cattgttgcc gccatgagct
     6901 ttgtcaaagc aggctttcac ggcgagaaac gccagttgtt gatgggtacg ccagcccgcg
     6961 ctgtatgcag tgttagtgac gacgagttac actggaaacg gttgaatacc aaagagtatc
     7021 aggatcttgt cgggcgctgt catgcatcgt tacatgaaac acagccactg aggcaaatgg
     7081 aggaaaatcg cccccgtttg caggggacga cggatgtgac gcctaaacgg tgatcttgcc
     7141 ggatgcgacc tggttgctgg ataagatgct acaacatcgc atctggcaag caggacgccc
     7201 ggtatcaacg agataaaatt aacgacgcat actctttgac aacatcatct gccacttctg
     7261 ctcgcggtta agttccgtga gcggaacggc aggaatacgc ggcgtctttc ttacgccgac
     7321 taatttatcc cggctgttat tgcgcaggcg cgcgtaaatt tgctcatcga tatcgaccac
     7381 ttttaccagt cgctggcact ggcatccccg tccttccagc ttattaggga tcatcttaac
     7441 ttcgcagctt atttctgcga cttccgacag aatataagtc agggtattaa ccgccttgct
     7501 gtgttcaata tcgaaatgaa tagagatctc tcttgctatc acccaccgat tttcagccat
     7561 catccattcg gcgattaaca agtagagtgg tttttcaaca tatccttcac acatacgaac
     7621 tccagtttca caaattaaaa gtctggctat gactcgtttt gaagcaatta aacaaggcca
     7681 tattaaaatt gtggatatat cgatagtatg caacttcacc gtcgataagt gtgagctaaa
     7741 tcctgcttat gtcatcaaaa atatcgattc cccaaaagat ttacttaatg ggcaaaaaaa
     7801 acggtcctca tcagagaacc gtattactta ttcgatcaaa ttagcggatg aaaaatatcc
     7861 gccatgacac gctattattt gatctgcgcg tgcatttcct gcaccgaaat tactttttcg
     7921 gtcgcgtcgg cattcagcgc catcgcggta gcgaaaccac cgttcagggt ggtgtcataa
     7981 tgcactttat attgcagcgc actgcgacgg atcacgcggg agtcttcaat cgcgcgacgg
     8041 cctgaagtgg tgttgatgat gtaggtatat tcgccattct tgatgcggtc ctgaatgtgc
     8101 ggacggcctt catgcacctt gttcaccaga cgcggattaa tgcccgcttc gcccagcaca
     8161 atcgccgtgc cgtgggtcgc atccagctcg aagccctgtt tcagcagttt tgccgccagg
     8221 tccaccacgc gttctttatc gccttcgcgc acggaaagca acgcacgacc gtgtttcttc
     8281 atggtggagt tgctgcccag ctgcgctttg gcaaacgctt cagcgaaggt gcggcccacg
     8341 cccatgactt ccccggtaga gcgcatttct ggccctaaca gcgggtcaac acccgggaat
     8401 ttattgaacg gcagcaccac ttctttcacc gagtagtacg gcgggataac ttctttggtt
     8461 acgccctgct cagccagcga tttgccagcc atcacacgcg ccgccacttt tgccagcgga
     8521 acgccggtgg ctttggagac gaacggaacg gtacgcgccg cacgcgggtt aacttcaatc
     8581 aggtagactt cgttgttttt caccgcaaac tgcacgttca tcagaccgcg cacctgcaat
     8641 tcgaaggcca gtttctgcac ctgctggcgc atcacatcct gaatttcctg acttaaggtg
     8701 taggctggca gagaacatgc ggagtcaccg gagtgcacgc ccgcctgctc gatgtgctcc
     8761 ataatgccgc caatcagcac catttcaccg tcgcagatgg catccacgtc aacttccacc
     8821 gcgtcatcga ggaagtgatc cagcaacact ggcgcatcgt tagacacgct gaccgccgtc
     8881 tggaagtagc gacgcaggtc agcttcgtca tagacgattt ccatcgcccg accgccgaga
     8941 acgtaagacg gacgtaccac cagcgggtag ccaatctctt tcgccttctc taccgccatt
     9001 tcaatagcgg taacggtggc gttcgccggt tgtttcagtt tcagacgctc aaccgcatgc
     9061 tggaagcgtt cacggtcttc tgcacggtcg atagcatccg ggctggtgcc gataaccggt
     9121 acgccagcgg cttccagtgc acgcgccagt ttcagcgggg tctgaccgcc gtactgaacg
     9181 ataacgcctt tcggcttctc aatacgcacg atttccagca catcttccag agttaccggc
     9241 tcgaagtaga ggcgatcgga ggtgtcgtag tcggtagaga cggtttccgg gttacagtta
     9301 accataatgg tctcaaaacc gtcttcacgc agcgccagcg aggcgtgtac gcagcagtag
     9361 tcgaattcga taccctgacc gatacggttt ggaccgccgc caagcaccat gattttttca
     9421 cggtcggtag acggattcgc ttcgcactct tcttcataag tggagtacat gtaagcggtg
     9481 tcggtggcaa actctgccgc acaggtatcc acacgcttgt agaccgggtg caggtcatat
     9541 tggtcacgca gcttacggat ttccgcttcg cgtacacccg cgagttttgc cagacgcgca
     9601 tcggcaaagc ctttgcgttt cagctggcgc aggaagtcag cgttcaggcc agtgatgccc
     9661 acttccgcca ctttctcttc cagacgcacc agttcttcaa tctgtaccag gaaccagcgg
     9721 tcaatgttgg tcaggttgaa gacgccgtcc acagacaggc ccgcacggaa agcatcggcg
     9781 atgtaccaga tacgctctgc gcctgcgtct ttcagttcgc gacggatttt ggttaacgct
     9841 tccgggtcat cgaggctcac tttcgggtcg aatccagtcg caccgacttc caggccgcgc
     9901 agcgcttttt gcagggattc ctgctgcgtg cgaccaatcg ccatcacttc gccaaccgat
     9961 ttcatctgag tggtcagacg gtcgttagca ccagcgaatt tttcgaagtt gaagcgagga
    10021 attttagtaa ccacatagtc gatggacggc tcgaaggagg ccggagtacg tccgccagtg
    10081 atgtcgttca tcagttcgtc gagggtgtaa cccaccgcca gtttcgccgc cactttagca
    10141 atcgggaaac cggtcgcttt cgacgccagc gccgaagaac gggacacgcg tgggttcatt
    10201 tcgataacaa tcaggcgacc gtttttcggg ttcaccgcaa actggacatt ggaaccaccg
    10261 gtttcaacgc cgatttcacg cagcaccgcc atcgaggcgt tacgcatgat ttgatattct
    10321 ttgtcggtca gcgtttgggc tggcgcgaca gtgatggagt caccggtatg gatgcccatc
    10381 gcatcaaagt tttcgataga gcagacgatg atgcagttgt cgtttttatc acgcaccact
    10441 tccatctcgt actctttcca gccgatcagc gactcatcaa tcagcagctc tttggttggg
    10501 gagagatcca gaccgcgggc gcaaatttct tcaaactctt cgcggttata agcgataccg
    10561 ccgccgctac cgcccatggt aaaggatggg cgaataatgc acgggaagcc cacgtcagcg
    10621 gcaaccgcca gcgcttcttc catcgtgtgt gcgataccgg aacgcgcggt ttccagacca
    10681 attttcttca tcgctacgtc gaaacgacgg cggtcttctg ctttatcaat cgcatcggcg
    10741 gtcgcaccaa tcatagtcac gccgaactct tccaacacgc cctgacgctc cagctccagc
    10801 gcgcagttca gcgccgtctg accgcccatc gttggcagca ccgcgtccgg gcgctctttt
    10861 tcaataatct tgcgtactac ttcccagtga atcggctcga tgtaggtcgc atcggccatt
    10921 tccgggtcgg tcatgatggt cgccgggttg gagttcacca gaatgacgcg gtaaccctct
    10981 tcgcgcaggg ctttacatgc ttgcgcgcca gagtagtcaa actcacacgc ctgaccgata
    11041 acaatcgggc ccgcacccag aatcaggata ctttttatat ctgtacgttt tggcatggct
    11101 cttttactcc tgattactta gcggttttac ggtactgctc aattaactcg ataaagtggt
    11161 cgaacaacgg cgctgcgtcg tgtggcccag ggctggcttc cgggtgaccc tggaagctga
    11221 acgccggttt atcagtgcga tgaatgccct gtaacgtacc gtcgaacagg gatttatgcg
    11281 tgacacgcag gtttgcaggt aatgttgctt catccaccgc aaaaccgtgg ttctgggcgg
    11341 tgatcatcac cacgtttttc tccacatctt taaccggatg gttgccgccg tggtgaccaa
    11401 atttcatttt gacagtcttc gcaccgctcg ccagcgccag cagctgatga ccgaggcaga
    11461 tgccgaatac cggaatatcg gtttcgagga atttctggat ggcggtaatg gcgtaatcgc
    11521 acggggccgg gtcgccagga ccgttggaga ggaagatgcc gtctggattc attttcagca
    11581 catcttccgc agaagtttgc gccggaacga tggtcaggcg acagcctcta tccaccagca
    11641 tccgcaggat gttgcgcttg gcaccaaaat cataagccac gacgtggaac ggcagctcgt
    11701 cttctttttt cgcttctggc aggccgccgg tcaacgtcca gctcccttgt gtccagctat
    11761 aggcttctgc ggtggtcact tcttttgcca gatccatgcc attcagacct gggaacgcgc
    11821 gggctttttc taacgccaac gccgcatccg ggttatcgcc tgcgataatg cagccattct
    11881 gtgcgccttt ctcgcgcagt aaacgcgtca gcttacgggt atcgatatcg gcaatcgcca
    11941 cgatgttatg gcgcttcagg taagaagaaa ggtcttcggt attacggaag ttgctggcaa
    12001 tcagcggcag atcgcgaatc accagacctt gtgcatgtac ctgagaagat tcttcatcgg
    12061 cgtcattagt gccgacattg ccaatatggg gataagtaag agtaacgatt tgacgagaat
    12121 aggaaggatc agtgaggatt tcttgataac cggtcattga agtattgaaa acgacttccc
    12181 caaccgccga acctgttgcc cctatggccc gaccgtgaaa ctgggttccg tcttccagaa
    12241 ccaatagcgc tgacttaatc aaaacaccct ccagagaata ttcactcact ttatttgcat
    12301 attaattcac aatgatgacg taaatcaatg caaatctgct taccgacgaa tttctggcaa
    12361 acggcggcat tctggagata tgggcgctaa aagtcaactt aatggcggga ttttttatat
    12421 tattttgtgg ctcatgcatg ttttacaggc attaagcgac aaaaagatca tataacctgt
    12481 tctggaaaac gcttgcgcag caagaagatt acatgaaata aaagcgagca gggaaaagtg
    12541 gagcaaatgg tcaaaattta cattaaaaca acaaaaaccg aagacaaaac atgtaatttt
    12601 tggcacttat agacaattta aaaacataac actaaaaata aaagggccaa aaattaaagc
    12661 cctttataat caataaatta aatgtgttat tcttgcacca taacaaatat tttgtggtta
    12721 caaactattg agatcaagca catctcgcat atcaaaaaga ccactttcct taccactcaa
    12781 ccacaaagcc gatcttaccg cgccgttagc aaatgtcatg cggctggacg ccttatgggt
    12841 gatctccaga cgctcgccaa tatcggcaaa catcgcggta tgttcgccaa cgatgtcacc
    12901 tgcacgcacg gtggcaaaac caatggtgcc aggcacacgt tcaccggtgt ggccttcacg
    12961 actgtagacc gcgcaatctt tcagatcttt atcaagggcg tgggcgatcg cctctcccat
    13021 tgccagtgcg gtgcctgacg gcgcatcaac tttatgtcta tgatgtgctt caataatttc
    13081 gatatcggtg tagtcaccca tcactttggc tgctttctcc agcagcttaa gcatgacgtt
    13141 aacgccaacg ctaaaattgg cggcaaagac aatcgcaata tcggcggcgg cgtcacgaat
    13201 cgcctgttta ccggcttcgt caaaccccgt agtgccgatc accatccctt tgccatgctg
    13261 gcgacaaaaa gcgagatggt tcagcgtacc ttccggacgg gtaaaatcga taaacacatc
    13321 aaaatcatct tttaccgcat cgaggctgct ttgcacggta acgcctgttt tccctgcacc
    13381 agccagctca ccggcgtcgc tgcccagtaa agaagatcct tcgcgctcca gcgccgcgcc
    13441 cagctgcacg ccttctaatg ccagcgccgc ctgaatcaac tggcggccca tacgcccccc
    13501 ggctcccgcg atggcaacgc ggatgtttgc atcatgcata gctattctct tttgttaatt
    13561 tgcatagacc gttttcagag taaccagcgc atcgctacgc cgccagccaa aaacgctgat
    13621 aattagaaat taatgataaa cagggaggga tatcagtgaa aggcatgagt cgatgaatga
    13681 ctgcatgcca ataacatgtg acaggttacg acgccagagc cagcacctca gccacccact
    13741 gctggaagcc tttcacatcc agatccaatg ccacctgtac attggctggc ttgcccaggc
    13801 aaccgtcgat atcaaccacc gtcgtgcctg aggtaaattc gccctgagtt tccactgcca
    13861 caaaacaggg tttgagagtg aacaggtccg ggcgcaccag ccaggcgatg gcgcagagat
    13921 cgtgcattcg caagccgctt tgcatactgc cgctacggta gtggctaaac agggcgtgaa
    13981 gcattttccc ggtacggttt aactgcggca gtgtagagag atagtcagga gttaatattg
    14041 cctgattggt gacatccaaa ccgcacatga cgatttcaat accactgcgg aagacacagg
    14101 cagcagcttc tggatcggca gcaatattaa actcggcgtt tggcgtacag ttgccgcgtc
    14161 cggcagaacc acccatgatc accagacggc gaatatacgg cttgcattcc gggcattgtg
    14221 aaagtaacag cgcaatattg gttaacgggc cgatggccac cagggtaaca ggctctggtg
    14281 cacgcatcag ggcatcccga atcgccagaa gcgccggtat cccgagcggc tttcggttgt
    14341 gctcaacaaa gtcgtagcca gccattcccg attcgccgtg cacagatgcc gcatcacgcg
    14401 gtgcgcgtac cagcggcaca gcggcccctt gggcgagcgg aatctccgca ttccagaaat
    14461 gcagcagttg cagggcattg cgggtagttt tctcaaccga gacattaccc gcgacggtgg
    14521 tcatcagttg caggtcgagt tcgggtgcaa aaatcgcggc ggcaatggcg acggcatcgt
    14581 caatgccggg gtcagtatcg aggaagatag gtaaacgcat gttttctcca taaaaaatgc
    14641 cggtaacaag accggcattt tcgcataact taggctgcta atgacttaat cgacttcacg
    14701 aatatcgaca cgcagctctt tcggcacttc gaaaacaatg ttttcttcac ggccttccag
    14761 cggaatggct tcaccaccgc ccagctgctg caaacgtgcc accacattct gcaccagaat
    14821 atccggagcc gatgcgcccg cagtcacgcc gacgcattta acctctttca cccactcttc
    14881 ctggatatct ttcgcatcgt caatcaaaaa cgcgcgtttg cccatacgct gggccagctc
    14941 cgccagacgg ttggagttgg aggagttttt cgaaccgacc accaacacaa cttccgcctg
    15001 ttctgccagg gcgcgtaccg cttcctgacg gttagtcgtg gcgtagcaga tgtcatcttt
    15061 gcgcggaccg acaattttcg ggaagcgttt acgcagcgcg tcgatcacat cagacgtgtc
    15121 atccaccgac agcgtggtct gggtcataaa ggagagcttc tcttcgtttt tgaccgtcag
    15181 tttccacaca tcgtccggcg attcgaccag atacattccc ccttccgggt tactgtactg
    15241 gcccattgtc ccttccactt ccgggtgccc ggcgtgaccg atgagaatag attcttcgcc
    15301 acggcgactg gcgcgggcga cttccatatg cactttggtc accagcggac aggtcgcgtc
    15361 aaataccgtc aaatcgcgac tttttgcttc gttacgtacc gcctgagaaa caccgtgtgc
    15421 ggagaaaatc aggatcgcgc cgtccggtac ttcgctaatc tgctcaataa agatagcccc
    15481 acgctcacgc aagctatcga ccacatagcg gttatgtacc acttcgtgac ggacatatat
    15541 cggtgcgccg taaatggcca gcgcgttttc aacaatgctg atagcgcggt ctaccccggc
    15601 acaaaaacca cgcgggttgg ccaacaggat ctgcatgtta cgcctccagt gccggatcga
    15661 tttccagcac ttcaatatca aaatgaacgg tctgcccggc cagcggatgg ttgaaatcaa
    15721 cggtaatgga gtcgccgtta atttcgcgga tcacgccagg catctcactg ccatccattg
    15781 cggtaaaaag catgattgcg ccaatttctg gctcgcctgc atccataaat tcacggcggg
    15841 agaagtactg aatcaggtcc ggtgacggca cgccaaacgc cgcatcgggc tccagcgaga
    15901 aggtggtttt atcgcccact ttcagcccca acaggtgttg ctccagccct tcagaaagag
    15961 aagcatcgcc caggcggaac agcgccggtt taccgttgtt gcgggtagac tcagcggtgg
    16021 tgccatcatc gagtttcagc gtgaagtgca ccaggacggc gctattgctc tgtacagatt
    16081 cagacatgca ggtttgctct tatattttga ggatttttgc cggatgcggc ctacgatttc
    16141 gcagcaatct gtaggcctta tccggcaggg tttattattg ttttttcgct ttagaaggca
    16201 aaaaaccttc cagcacaatc agtgccgcac cgacacaaat ggcagtatcg gcaaggttga
    16261 aggtggcgaa gtgccagtcg ccgacgtaga agtcgatcat atcgacaacg aagccgtgcc
    16321 acaggcggtc gaacaggttg cccagcgcgc cgccaataat cagcgcataa gcgatattgt
    16381 ttagcttctg cgtggccttc gagcgataca tcatcactgc caggatcacg ctaataccaa
    16441 tcgcaatacc ggcaaagaac caacgctgcc agccgccgct atcggcaagg aaactaaacg
    16501 ccgcgccata gttacgcgca taatgcagat taagcgacgg gaacagcggg accgtatccc
    16561 ccagagcaaa gttctggagg atcaggtatt tgctgcccag atcgataatc agcacgacta
    16621 ccaccagcca cagccagcgt agccctgttg aacagatcga ttgactcatc aggcaaactt
    16681 acgtttttca ccgtcaccgg cgacgttgct gacacagcgg ccgcagattt ctgcgtgttc
    16741 cgccaccttg ccgacatcct gggtgtagtg ccagcagcgt gggcacttct caccttcggc
    16801 tttactcaac gcgactttca gccctttgag tacttcgctc tgctgagcat cagcaggtgc
    16861 gtcgttatag tctgcaacgg tagcgccgga ggtcaacagg acaaatcgta attcatcgcc
    16921 cagcgcggtc agtttcgccg acagttccgg ttctgcatac aaggttactg ccgcttccag
    16981 cgagccaccc actttcttgt cggcacgcgc ttgctcaatg actttgttca cttcgccacg
    17041 cactttcaac agctcgtccc agaacgcatc gttcatcgct tcactgtctg ccaggccaaa
    17101 caggccttcg taccactcac cggtgaagac gtatttttca cgttcgcccg gcaggtagcc
    17161 ccacacttca tcagcggtga aggagaggat tggtgccatc cagcgcacca gcgcttctgc
    17221 gatgtgatac agcgcagtct ggcagctacg acgcgccaca ctgtccgctt tggcggtgta
    17281 ctgacggtct ttgatgatgt cgaggtagaa ggaacccatc tcaacggagc agaagcgcat
    17341 cagacgctgt accacttcgt ggaaatcgta tgcttcgtac gccttgagga tgtcttcctg
    17401 tgccgctttc gcacaaccta cggcccagcg atccagtacc accatctctt ccggtttcac
    17461 catatctttt gctggatcaa aaccgttcag gtttgccagc aggaagcgcg cggtgttacg
    17521 gatacgacga tagctatcgg cagcacgttt caggatctcg tcagaaacgg ccatttcacc
    17581 ggtgtagtcg gttgatgcca cccacagacg cagaatatcc gcgcccagtt tgttcatcac
    17641 atcctgcggc gaaacggtat tgccgatgga tttagacatc ttgcgaccct gaccatccac
    17701 ggtaaagccg tgggtcagta cctgacgata cggcgctttg cctttcatcg cggtggagat
    17761 cattagggaa gacatgaacc aaccacggtg ctggtcagaa ccttccagat acatgtccgc
    17821 tgcgtgaccg gcaaattccg gacgcacgtc aacaacagaa gagtgggtag atccggagtc
    17881 aaaccataca tccaatgtgt ccggcacttt cacgtactga tcagcttcat cgccgaggat
    17941 ctctttcgca tcaagatccc accacgcctg gatgccgtca acttcaacgc gttttgccac
    18001 ttcttccatt agttcgagag tacgcggatg cagctcttcc gtgtctttgt gcacgaacag
    18061 tgacatcggt acgccccagg tgcgctgacg ggagatacac cagtcaggac ggttagcaac
    18121 catcgactcg atacgcgcct ggccccagtc cgggatccac tgcacgcctt tgatctcttt
    18181 cagtgactgc gcacgcagac ctttctgatc catgctgacg aaccactgcg gcgtcgcgcg
    18241 gaagatgatc ggcgttttgt gacgccagca gcacggatag ctgtgctgca ttttctcaac
    18301 gtgcagcagc gcgccttttt cctgcagcag cgcaacgacg atgtcgttcg ctttgaagac
    18361 gttcacgcca tccagcgtcg gataagtgcc cggcagataa gtgccgtccg ggccaaccgg
    18421 gttagcggtt tccaggccgt atttctgacc gatcacatag tcgtccgggc cgtggccagg
    18481 cgcggtgtga acggcaccgg taccggcatc cagggtaacg tgatcgccga ggattgccgg
    18541 aacgtcgaag cccataaacg gatgggtaaa gcgcagcaac tcaagctccg caccttttac
    18601 cgtgccgaga atggtgtaat cggtcacgcc gatacgctgc attacgcttt caaccagatc
    18661 tttcgccaga atcacggcct gaccgtcaat ctgcaccagc gcatagtcga aatcaggtgc
    18721 aatagagatt gcgcggttcg caggcagagt ccacggcgta gtggtccaga tcaccagcga
    18781 gattgggccg ttaacgttgc ttacggcaaa ttttgctttc agtgcatcct gatcgaccgc
    18841 ctgaaaagca acgtcgatgg acggagaagt tttgtcgtaa tactcaactt ccgcttccgc
    18901 cagcgcagaa cggcagtcaa cgcaccagtg aactggcttc gcgcctttgt gcaggtgacc
    18961 gttgccgatg attttgccca gcgcgcggat gatgttggct tcagttttga agtccatggt
    19021 caggtacggg tgcgaccagt cgcccagcac gcccagacgg ataaagtctt tgcgttgacc
    19081 gtcaacctgg gtcgccgcgt attcgcggca cttggcgcgg aactcggcgg cggtgaattt
    19141 ctcacccggc ttaccgtatt cttgctctac tttcagctcg atcggcagac cgtggcagtc
    19201 ccagccaggc acatacggcg agtcataacc ggaaagccct ttggacttca cgataatgtc
    19261 tttcagaatc ttgttaaccg agtgaccaat atgaatgctg ccattcgcat aaggagggcc
    19321 atcatgcaga atgaaggttt ttttgccttt tttagccgca cggatgatgc cgtacagatc
    19381 atcatcagtc caacgcgcca gcattcccgg ttcgcgcttg gcgagatcgc cacgcatcgg
    19441 gaaccctgtt tccggcaaat tcagggttga tttatagtca ctcatcagat tctcggttcc
    19501 gtgtttcggt ttgattacat aacaggctta agccggtttt gttagcccaa aaaattcgcg
    19561 ggcggttaat tcatcacgcg caatctgcgc tttcagttcg tccagcgacg caaatcgctg
    19621 ctcattgcgt atttttttac gcagcactac ttgtatatgg cgaccgtaaa ggtccattgc
    19681 aacatctaac aaatgcactt ccagctgctg gcgaataccg gcaaccgttg ggcgtgttcc
    19741 aatgtttgcc acgccgggta acggcttttc accgaggccc agcacttcta ccgcataaac
    19801 ccctttcacc ggggaaacct gacggcgtag cggtacattc gccgtcggga aacctatagt
    19861 gcgccctaat tcatcaccgt ggactacacg cccggagatg gcaaacgggt gccccagtaa
    19921 actttctgcc agagccagat tgtcatccgc cagcgcctga cgcacagcgg tgctgctgat
    19981 acgcacgcca ccttcgcaaa aagtttgcgt actggttata tcgaagccgt attccatgcc
    20041 agctttctgt aataacaaga aatcgccttc acgaccagcg ccaaagcgga aatcatcacc
    20101 tacggcaaga aattttacgc gcaaatgctt caccagaaga tcgctgatga aattttgcgc
    20161 ggttaacgcc gcgaaacgcc tgtcgaaacg cacgcacagc acgtaatcaa cgccacactc
    20221 tgcaaggtaa cgcagttttt cccgcagccg ggtcagccgt gccggggctt tatcggtagc
    20281 aaacagttcc agtggttgag gttcaaaaag catcaccatc accggtaagt tgcgcttgcg
    20341 cccttcttcc tgcaagccct gtaacagcgc gcgatgaccg cgatgcacgc cgtcgaaatt
    20401 accaatagtc agcacacacc cttcttgcgg ggcctggctg agattatgta tgccgcgtat
    20461 cagcttcatg tctggctcaa aacagtgaaa atcgtccgag tataccttgt acagcggtca
    20521 aggttaaccg gcgattgagt accgaaatca gaaagaagtg gcttttcatt gccatggcgc
    20581 aaatcacggg aagaaagtga ccgcctgctg caatttttat cgcggaaaag ctgtattcac
    20641 accccgcaag ctggtagaat cctgcgccat cactacgtaa cgagtgccgg cacattaacg
    20701 gcgcttattt gcacaaatcc attgacaaaa gaaggctaaa agggcatatt cctcggcctt
    20761 tgaattgtcc atatagaaca catttgggag ttggaccttg gctaatatca aatcagctaa
    20821 gaagcgcgcc attcagtctg aaaaggctcg taagcacaac gcaagccgtc gctctatgat
    20881 gcgtactttc atcaagaaag tatacgcagc tatcgaagct ggcgacaaag ctgctgcaca
    20941 gaaagcattt aacgaaatgc aaccgatcgt ggaccgtcag gctgctaaag gtctgatcca
    21001 caaaaacaaa gctgcacgtc ataaggctaa cctgactgca cagatcaaca aactggctta
    21061 atcgccaatt tgctgaagct ttgtgaaaaa gcccgcgcaa gcgggttttt ttatgcctgc
    21121 tgcttttatg atgcgtgaga gaacacacta ccggttgcca gatagtcaaa agatgctggc
    21181 ttttgcagac atgccgttac actttctgaa ctcttcgtgg cttaagaatc gtagaaccat
    21241 cggctgatac attctgaaca agaatatcta ctcaatctcc agactcccct acaaagaatg
    21301 tggatattgt gatacacatt gaggtattaa acttaagacc a
//
LOCUS       DAZFQD010000085        21334 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.085, whole genome shotgun sequence.
ACCESSION   DAZFQD010000085 DAZFQD010000000
VERSION     DAZFQD010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21334
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.085"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(35..1333)
                     /gene="eno"
                     /locus_tag="ACHKTW_003181"
     CDS             complement(35..1333)
                     /gene="eno"
                     /locus_tag="ACHKTW_003181"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFO6464152.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(1421..3058)
                     /gene="pyrG"
                     /locus_tag="ACHKTW_003182"
     CDS             complement(1421..3058)
                     /gene="pyrG"
                     /locus_tag="ACHKTW_003182"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine hydrolyzing CTP synthase"
                     /protein_id="HFO6464153.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(3286..4077)
                     /gene="mazG"
                     /locus_tag="ACHKTW_003183"
     CDS             complement(3286..4077)
                     /gene="mazG"
                     /locus_tag="ACHKTW_003183"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HFO6464154.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     ANEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(4148..4483)
                     /gene="mazF"
                     /locus_tag="ACHKTW_003184"
     CDS             complement(4148..4483)
                     /gene="mazF"
                     /locus_tag="ACHKTW_003184"
                     /EC_number="3.1.27.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease MazF"
                     /protein_id="HFO6464155.1"
                     /translation="MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTG
                     MCLCVPYTTQSKGYPFEVVLSGQERDGVALADQVKSIAWRARGATKKGTVAPEELQLI
                     KAKINVLIG"
     gene            complement(4483..4731)
                     /gene="mazE"
                     /locus_tag="ACHKTW_003185"
     CDS             complement(4483..4731)
                     /gene="mazE"
                     /locus_tag="ACHKTW_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin MazE"
                     /protein_id="HFO6464156.1"
                     /translation="MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLII
                     EPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKEVW"
     gene            complement(4809..7043)
                     /gene="relA"
                     /locus_tag="ACHKTW_003186"
     CDS             complement(4809..7043)
                     /gene="relA"
                     /locus_tag="ACHKTW_003186"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP diphosphokinase"
                     /protein_id="HFO6464157.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            complement(7091..8392)
                     /gene="rlmD"
                     /locus_tag="ACHKTW_003187"
     CDS             complement(7091..8392)
                     /gene="rlmD"
                     /locus_tag="ACHKTW_003187"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFO6464158.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVAVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHDVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILVPQLEALLPKVRACLGSLQVMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVQPEDRVLDLFCGMGNFTLPLAKQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            8449..11205
                     /gene="barA"
                     /locus_tag="ACHKTW_003188"
     CDS             8449..11205
                     /gene="barA"
                     /locus_tag="ACHKTW_003188"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HFO6464159.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISSVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSTQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQQ
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLVGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(11437..12777)
                     /gene="gudD"
                     /locus_tag="ACHKTW_003189"
     CDS             complement(11437..12777)
                     /gene="gudD"
                     /locus_tag="ACHKTW_003189"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008872 - glucarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HFO6464160.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGRTLGEYKNVLTLVRNTFADRDAGGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGDRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYHKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(12798..14138)
                     /locus_tag="ACHKTW_003190"
     CDS             complement(12798..14138)
                     /locus_tag="ACHKTW_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HFO6464161.1"
                     /translation="MTTQSSPVITDMKVIPVAGHDSMLLNIGGAHSAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVDAIPMVLGQEVARLNKVVQQVHIGNQAADFDTFGKGA
                     WTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQRDAITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKRFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAMFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPGGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(14140..15492)
                     /gene="gudP"
                     /locus_tag="ACHKTW_003191"
     CDS             complement(14140..15492)
                     /gene="gudP"
                     /locus_tag="ACHKTW_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HFO6464162.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPAQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQNTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCSFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            complement(15927..16376)
                     /locus_tag="ACHKTW_003192"
     CDS             complement(15927..16376)
                     /locus_tag="ACHKTW_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFO6464163.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEQWGTLLS"
     gene            complement(16394..17176)
                     /gene="truC"
                     /locus_tag="ACHKTW_003193"
     CDS             complement(16394..17176)
                     /gene="truC"
                     /locus_tag="ACHKTW_003193"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HFO6464164.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGSQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(17176..17505)
                     /locus_tag="ACHKTW_003194"
     CDS             complement(17176..17505)
                     /locus_tag="ACHKTW_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HFO6464165.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWVLIPRMHDLLDNKQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            complement(18127..18672)
                     /gene="syd"
                     /locus_tag="ACHKTW_003195"
     CDS             complement(18127..18672)
                     /gene="syd"
                     /locus_tag="ACHKTW_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HFO6464166.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTHLASNLAEFLNQLKPLL"
     gene            18740..19588
                     /gene="queF"
                     /locus_tag="ACHKTW_003196"
     CDS             18740..19588
                     /gene="queF"
                     /locus_tag="ACHKTW_003196"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708588.1"
                     /note="Catalyzes the NADPH-dependent reduction of
                     7-cyano-7-deazaguanine (preQ0) to
                     7-aminomethyl-7-deazaguanine (preQ1) in queuosine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA];
                     GO_function: GO:0043867 - 7-cyano-7-deazaguanine
                     tRNA-ribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HFO6464167.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDANLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSANLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKVSVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATSGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            19700..21064
                     /gene="ppnN"
                     /locus_tag="ACHKTW_003197"
     CDS             19700..21064
                     /gene="ppnN"
                     /locus_tag="ACHKTW_003197"
                     /EC_number="3.2.2.10"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase PpnN"
                     /protein_id="HFO6464168.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
BASE COUNT         5215 a         5730 c         5084 g         5305 t
ORIGIN      
        1 gaggctggca tttttaaatc agataaagtc agtcttatgc ctggcctttg atctctttac
       61 gaccgttgta cggtgctttt tcgcccagag cttcttcgat acgaatcagc tggttgtatt
      121 tagcaacacg gtcagaacgg ctcatagaac cagttttgat ctggcctgca gcagtaccaa
      181 cagccaggtc agcgatggta gcgtcttcag tttcgccaga acggtgagag ataactgcag
      241 tgtagccagc atctttcgcc atcttgattg cagccagagt ttcggtcaga gaaccgatct
      301 ggttgaattt gatcaggatg gagttagcga tacctttttc gataccttct ttcaggatct
      361 tggtgttggt tacgaacagg tcgtcaccaa ccagctggat tttgtcgccc agaactttgg
      421 tctggtatgc gaaaccgtcc cagtcagatt cgtccagacc gtcttcgata gaaacgatcg
      481 ggtactgttt ggtcagttct tccaggaagt gagtgaattc ttcagaggtg aacgctttgt
      541 tgccttcgcc agccagaacg tatttaccat ctttgtagaa ttcagaagct gcgcagtcca
      601 tcgccagagt gatgtctttg cccagttcat aaccagcagc tttaacagct tcagcgataa
      661 cagccagagc ttcagcgttg gaacccaggt tcggcgcata gccaccttcg tcaccaacag
      721 cagtgttcat gcctttcgct ttcagaactt ttgccaggtg atggaaaact tcagaaccca
      781 tgcggatggc ttctttcaca gttttcgcgc caaccggctg aatcatgaat tcctggatat
      841 caacgttgtt gtcagcgtgc tcaccaccgt tgatgatgtt catcatcgga accggcatag
      901 agtatttgcc cggagtaccg ttcagttcag cgatgtgctc gtacagcggc atacctttag
      961 cagctgcagc agctttggcg ttagccagag atacagccag gattgcgttc gcgccgaatt
     1021 tggatttgtt ttcggtgccg tccaggtcga tcatgatctt gtcaatgcca gcctgatctt
     1081 tagcatcttt gccaatcagc gcctgagcga tcgggccgtt taccgcagca acagctttgg
     1141 ttacgccttt acccaggaaa cgggatttgt cgccatcgcg cagttccaga gcttcacggg
     1201 aaccagtaga agcacctgac ggagcagctg ccataccgac gaaaccaccc tccagatgta
     1261 cttcggcttc aacagtcggg ttaccacggg agtcgatgat ttcacgaccg atgattttta
     1321 cgattttgga cattaggttt tcctcaagtc actagttaaa ctgaaactcc agacaaacaa
     1381 cgcgtaccca gggtacgcgt tgccgttcta acttttttac ttacttcgcc tgacgcttct
     1441 ggaactcgct ggcggctttt acaaagccag caaacagcgg gtgaccatca cgtggagtag
     1501 aagtaaactc cggatggaac tggcaagcca cgaaccacgg gtgattcgga acttcgatga
     1561 tctcgaccaa ctgatcatcc ccggaacggc ccgcaacgcg cagacctgca tcttcaatct
     1621 gtttcaacag catgttgttg acttcgtaac ggtgacgatg acgctcaaca attgtcggcg
     1681 cattgtacag ctggcgaacc aggctatcgt caaccaactg gcactgctgt gcgccgagac
     1741 gcatggtacc gccgagatcg ctcttctcgc tacgaacttc aacgttgccg ttttcatcgc
     1801 gccactcggt aatcagcgcc acaaccgggt acttacagtc tggcacaaat tccgtagagt
     1861 tggcgttctc catgttggca acatggcgag cgtaatcaat taacgccacc tgcataccca
     1921 ggcaaatgcc cagataagga atattgttct cacgcgcaaa acgcgcggtc gtaatcatgc
     1981 cttctacgcc acgatagcca aaaccgccag gtactaggat tgcgtccaga cctttaagga
     2041 tttcaacgcc gcgcgtttca acatcttgtg aatcgatcag tttgatgttg acgctgacac
     2101 gattcttcag cccaccgtgt ttcagtgctt cgatcactga tttataagca tccggcagtt
     2161 caatgtactt gccgaccata ccgatggtga cttcacttac cgggttcgct tcttcgaaga
     2221 taacctgttc ccattcggac agattcgctt ccgggcagtt taagctgaat cgtttacaaa
     2281 tataatcgtc cagcccctga gatttcaaca ggcccggaat tttatagatg gaatcgacgt
     2341 ctttcagaga aataaccgct ttttccggaa cattacagaa caatgcaatc ttcgcacgtt
     2401 cgttcgccgg aacagcgcga tctgaacgac aaatcaggat gtcaggctgg ataccgatgg
     2461 agagcagctc ttttacagag tgctgagtcg gtttggtttt gacttcacca gacgctgcca
     2521 tgtacggcac cagcgtcagg tgcataaaca gagtgtgctc acggccaatt tcaacagcca
     2581 tctggcgaat cgcttcgagg aacggcaagg attcgatatc acctactgta ccgccgattt
     2641 ctaccagtac tacgtcatga ccttcgccac cttccagcac gcgctctttg attgcgttag
     2701 tgatgtgcgg aataacctgc acggttgcgc cgaggtagtc accgcggcgt tctttacgca
     2761 gaacgtcaga gtagatacga cccgtggtga agttgttgcg gcggctcatt ttggtacgaa
     2821 tgaaacgctc gtagtgcccc aggtccaggt cggtttcagc gccgtcttca gtaacgaaca
     2881 cttccccgtg ttggattggg ctcatagtac ctggatcgac gttgatgtac ggatccagtt
     2941 tcatgatggt cacattgagg ccacgggctt caagaatggc tgcgagggag gctgcggcaa
     3001 tgcctttacc cagagaggat acgaccccgc cggtcacaaa aatatagttc gttgtcatgc
     3061 tgaacctgag aagttaggtt gaaaagacga tggaataacc aggacgggaa agcagtatac
     3121 ccgaacatga cctgtgccac aaactttcat tatccctcct cttcgccagc gcactattga
     3181 aatcaggagt gagaaaatag cccctttggg gtaaatgttt ttgacgcaaa tcaagcgctt
     3241 gtcatttaaa aaatgacaca aatggcgctt gaccgcgtaa ttcccttaga gatcaatttc
     3301 ctgccgtttt acctgttgcc agacttcttc cattgtttcg aggtcaacac ctgtcatttc
     3361 cagtccacgc gcggcaacaa tacgctccac ttcgcgaaaa cgacgctcga atttttcgtt
     3421 cgctttttgc aatgcgattt ctgctttcgt ccctaaatgg cgagccagat taaccgtggc
     3481 aaacagcagg tcccccattt cctcctccag tttagcctgg tcgacaacag cctgccgcgc
     3541 ttcgtacatc acctcgtcga tctcttcgta gactttatcg actaccggac caagcgtcgt
     3601 ccaatcgaag ccaacgttgg cgcaacgttt ctggattttt tgcgcacgca ttaaagccgg
     3661 taaactacga ggaatatcgt ccagcgccga atgctgcgct ttctgcgcgc gctcttcggt
     3721 tttgatttgc tcccaacggg caagcacttc actactgttt tcggcagaac tatcagcaaa
     3781 aacatgcgga tggcgacgct ctaatttatc gctaatagca gcgcaaatat cattaaagtc
     3841 aaagcgccct tcttcctgag ccatttgcgc gtaaaacacc acctggaata gcagatcgcc
     3901 cagttcaccg cgaagatcgt caaaatcttc acgggcgatg gcgtccagca cttcgtaggt
     3961 ttcttcaagg gtgtaaggcg caatggtggc aaatgtctgc tctttatccc acgggcagcc
     4021 gttttccgga tcgcgcaggc gctgcataat agtgagcaaa cggtcgattt gattcattga
     4081 attgtcctga aaattgcggg tctgtcaggt ggaaacctgt gaccagaata gaagtgagtt
     4141 agtaacacta cccaatcagt acgttaattt tggctttaat gagttgtaat tcctctgggg
     4201 caactgttcc tttcttcgtt gctcctcttg cccgccaggc gatacttttt acctgatcag
     4261 ctaacgctac gccatcacgt tcctgaccgg ataaaacaac ttcgaacgga tatccttttg
     4321 attgcgttgt ataaggaaca cacagacaca tacctgtttt gttgttgtac atgaaaggac
     4381 tcaggacaac agctggacga tgtccagctt gctcgctacc ttttgtcggg tcaaaatcaa
     4441 cccaaatcag atcgcccata tcgggtacgt atcggcttac cattaccaga cttccttatc
     4501 tttcggctct ccccagtcga tattctcgtg gaggttttcc ggcgtgatgt cgttgaccag
     4561 ttcagcaagc gtaaatacgg gctctttacg cactggctca ataattaatt tgccatccac
     4621 caggtcaatc ttcacttcat catcaatatt cagattgagc gcctgcatta acgtagccgg
     4681 gatccgcacc gccggtgaat ttccccaacg ctttacgcta ctgtggatca taaccctttc
     4741 ctcaaaccgc tatcatatgt agatacagta tatatcaatc tacattgtag atacgagcaa
     4801 atttcggcct aactcccgtg caaccgacgc gcgtcgataa catccggcac ctggttgagt
     4861 ttacccagca cgcgccccag cacttgcagg ttgtaaatct caatggtcat gtcgatggtc
     4921 gccagttgct gtttggtgtc gctacggctg gcaacgccaa gcacgttcac cttctcgttg
     4981 gcgagaatgg tcgtgatatc acgtaacaac ccactacgat cattagccac cacacggacc
     5041 accagcgaat atccggcgga gtagctctca ccccataccg cgtcaacaat gcgttctggc
     5101 gcatgggagc gcagttccgc cagttgttcg caatcggcgc ggtgtactga aataccgcgc
     5161 ccctgggtaa tgaagccgac aatctcatct ccaggaatcg gctggcagca gcgcgcgatg
     5221 tggtgcatca ggttgccaac accttcgacc accacgcgac cgttatcttt actgcggttt
     5281 tgcggcgtgt agcttttttg ctgaagttgc ttcagcgcgg cggcgtcctg ctcttcggca
     5341 ctcggcttat taaattgcga ttgcaggaag ttcaccatct gattgagacg gatatccccg
     5401 ccaccaatcg ccgccagcaa ctcgtcgaca tcattaaagt tgtaacgcgg cagcagatgt
     5461 ttttctgctt ctttcaggct gatccccaga tgttccagct cgtcgtcaag gatttgccgc
     5521 ccagccagaa tgtttttgtc acggtcctgt ttacggaacc aggcgtgaat tttcgaacgc
     5581 ccacggctgg ttgtgacgta accgaggttt gggtttaacc agtcacggct ggggttcggc
     5641 tgtttctggg tgataatttc aatctggtcg cccatctgca gctggtaggt gaacggcaca
     5701 atgcgcccgc caatttttgc cccgatgcag cggtgtccga catcactgtg gatgtggtaa
     5761 gcgaagtcca gcggcgttga tcccgcaggc aaatcaacga catcaccttt cggcgtaaag
     5821 acatacaccc ggtcgtcaaa gacctggctg cgtacttcgt cgagcatttc gccggaatca
     5881 gccatctctt cctgccacgc aatcagttta cgcagccagg caatccggtc ttcatgtccc
     5941 gaacgtgcgc cgccagctgc cgcgccctct ttatatttcc agtgcgcagc aacacccaac
     6001 tctgcatctt catgcatctg tttggtgcgg atttggatct caacggtttt gccacctggc
     6061 cccagaacca cggtatgaat agactgataa ccgtttggtt tcgggttagc gacataatcg
     6121 tcaaactcat ccggcaggtg gcgatagtga gtgtgcacta tccccagtgc ggcataacaa
     6181 tcctgtaaac gctcggcgac aatacgtacc gcacgcacat caaacagctc atcgaaggcg
     6241 aggttctttt tctgcatttt gcgccagata ctgtagatgt gtttcggacg accatacact
     6301 tcagctttaa cgccttcagc tttcatctca gcgcgcagat gaccaacgaa ctcttcgatg
     6361 tagtgttcgc ggtcgagacg ccgttcatgc agcagtttgg caattcgttt gtattcggtt
     6421 ggatggaggt aacggaagca gtaatcttcc agttcccatt tcagttgtcc gattccgaga
     6481 cggttagcca gcggtgcgta gatgttggta cactcttttg ccgccagtac acgttcatct
     6541 tccggcgcat cttttacttc gcgcagatga gcaatacgct ccgccagttt gatgactacg
     6601 cagcgaaaat catcgaccat cgccaataac atccggcgaa cgttatcgac ctgttcggag
     6661 gaaacagaat cagtgtgcgt cgctttcagc tggcggatcg ccgccatatc acgcacgccg
     6721 tgaataaggt taacgaccga cttaccgacg ctctcacgca gcacatcttc gctgactacg
     6781 ttggcatcag ccagagggaa cagcagcgcc gcccgcagcg tgtcaatgtc catacttaat
     6841 gtcgagagga tctccaccat ctcaacacca cgccacaata acagactggc atccggatgc
     6901 ccctgcgtct gttgcagaca atacgcccag gtttcggcta agcactcaca cgacttctgg
     6961 ctggtaatac ccagacttgc gatccatttt tccggatcaa attcaccagc cttattgata
     7021 tgtgcacttc ttaccgcaac cattgtcctc tcctttaggg accagacctg ccgaaatcgg
     7081 caaatcgtaa ctatttaacg cgcgagaaaa gtaccatcga ttccagatgt cccgtgtgtg
     7141 ggaacatatc cagcatcgcc agtcgcgcaa tggtatatcc tgcttttaat aacgcttcgc
     7201 tatcccgagc cagcgttgca gggttacagg atacataaac tatacgaata ggttccagtt
     7261 ttataatttg ctgcataaca cctgcggcac ctgctcgcgc cgggtccagc aacactttat
     7321 cgaagccgtt tttcgcccac ggctgctttg tgacatcttc ttcaagattt tcgtgataaa
     7381 acgtcacatt ctgtaaacca ttaagacgcg cattctgctg gcctttttcc accagcgccg
     7441 gaacaccttc tacaccgacc acactggctg cttgtttcgc caatggcagt gtaaagttgc
     7501 ccataccgca gaacagatcc agtacgcgat cttcaggttg cacatccagc cattccaacg
     7561 cacgcgctac cattttttgg ttcacacccg cattgacctg aataaaatcg cgcgggctaa
     7621 aagttaagcg caacccgttt gagtcatacc agggcatctc accagagacg gtttcgagta
     7681 tctcactatc gggggcgaga tacagatcca ggccttcaga atgcgaaaag cgttccagtt
     7741 tttcgcgatc tgccgaactt agcggtgcgg tatggcgcaa aatcatcagc gtgccgctgg
     7801 ttgcctgtac cagttcaaca tgaccaagat ggcgcataac ttgtaagctg cccaggcatg
     7861 ccctgacttt gggcagcaat gcttcaagtt ggggcactaa aatggggcat tgtttaacgt
     7921 cgacaatgtc actggagccc gctttgcgaa accccatctg aagttgctgt gttttcggta
     7981 agtagttcag acttaaacgc gcgcggcggc gatagcccca gggaacatcg gcgatcactt
     8041 cagagacatc gtgtttcatt aatcgggcga gtgccgcact tttgcttcgc tgctgtaaat
     8101 ccacgctggc gtgttgttgc tgacagccgc cgcatacgcc aaaatgagga cagcgtggtg
     8161 tttcgcgttc cgggctatcg cttaaccggc gtacgacttt agcgcgggca tactgttttt
     8221 tatcttcagt aacagcaact tccgcgtttt cctgcggcaa taatccgggg ataaataacg
     8281 ttttgccgtt atgtcgcgcc acgccctgac caaaagagtc gaggtcgttg actgaaacgg
     8341 ttatgatctg acgcgtcgtc gtgcgtcgtt ttgcagagta gaattgcgcc attggcgaga
     8401 ctttctcaat ttaacagtgt gaccttaatt gtcccataac ggaactccat gaccaactac
     8461 agcctgcgcg cacgcatgat gattctgatc ctggcaccga ccgtccttat tggtttattg
     8521 ctgagtatct ttttcgtcgt gcatcgctat aacgacttgc agcgtcaact ggaagatgcc
     8581 ggtgccagca ttattgagcc gcttgcagtt tctactgaat atggcatgag cctgcaaaat
     8641 cgcgaatcta tcggtcagtt aataagcgta ctgcatcgtc gccattccga tattgttcgc
     8701 gcgatttcgg tttatgatga aaataaccga ctctttgtca cctccaattt tcatcttgat
     8761 ccctcatcaa tgcagctcgg cagcaacgtg ccgtttcctc gccagctcac tgtcactcgt
     8821 gacggcgata ttatgatcct ccgcacgccg attatttctg agagttactc ccccgacgaa
     8881 tcgcccagta gcgatgccaa aaatagtcaa aatatgttgg gatatattgc gctggagctg
     8941 gatcttaaat cggttcgctt gcagcaatat aaagagatct ttatttccag cgtgatgatg
     9001 ctgttttgta tcggtattgc gcttattttt ggctggcgct taatgcgcga tgtaaccggt
     9061 ccgattcgca acatggtgaa taccgtcgac cgcatccgtc gcgggcaact cgacagccgg
     9121 gtggaaggat ttatgctcgg cgagctggat atgctgaaaa acggtatcaa ctcgatggca
     9181 atgtcgctgg ctgcttatca cgaagagatg cagcacaata tcgaccaggc gacgtccgat
     9241 ctgcgtgaaa cgctggagca gatggaaatt cagaacgttg agttagatct ggcgaaaaag
     9301 cgcgcccagg aagcggcgcg tattaaatcc gagtttctgg caaatatgtc acacgagctg
     9361 cgtacaccac tgaatggtgt tattggcttt acccgcctga cgctgaaaac agaattaaca
     9421 ccaacgcagc gcgatcacct gaatacgatt gaacgttcgg caaataattt gctggcaatt
     9481 attaatgatg ttctcgactt ctcgaaactg gaagcaggta agctgattct ggaaagtatt
     9541 ccattcccac tacgcagcac gctggatgaa gtcgttactc tgctggcaca ttcttctcac
     9601 gataaagggt tggaactgac gctcaatatt aaaagcgacg tgcctgataa cgtgatcggc
     9661 gacccactgc gattacagca aatcatcact aacctggtgg ggaatgcaat taaattcacc
     9721 gagaatggca acattgatat tctggtagaa aaacgtgcgc tgagtaatac caaagtgcag
     9781 attgaagtgc agattcggga taccggcatt ggtattcctg aacgcgatca atcgcgctta
     9841 ttccaggcct tccgacaggc tgatgccagt atttcccgcc gtcatggtgg caccggtctg
     9901 gggctggtga ttacacaaaa actggttaat gaaatgggcg gcgatatttc gttccatagc
     9961 cagccgaatc gcggttcaac tttctggttc cacattaatc tcgatctgaa cccgaacatt
    10021 attatcgaag ggccatccac ccagtgcctc gccggtaagc gcctggccta tgtcgaacca
    10081 aactccgcag cagcgcaatg cacgctggat attttaagtg aaacgccgct ggaagtggtt
    10141 tatagcccaa cgttctccgc gctgcctccc gcgcattacg acatgatgtt gttaggcatc
    10201 gcggtgacct tccgcgagcc gctaacaatg caacatgagc gattagcgaa agcggtatcg
    10261 atgaccgatt tcctgatgct ggcacttcct tgccatgcac aagtcaatgc agaaaaactc
    10321 aagcaagatg gtatcggcgc gtgtctgctg aaaccattaa cacctacgcg cctgttgcct
    10381 gccctgacgg aattttgtca tcacaaacaa aacacgcttt tgcctgtaac cgatgaaagc
    10441 aagctggcaa tgacagtcat ggcagttgat gacaaccccg ctaacctgaa acttatcggc
    10501 gcattgctgg aagatatggt gcaacatgtg gaactttgcg atagcgggca tcaggcggtt
    10561 gaacgggcga aacagatgcc gttcgatttg atcttaatgg atattcaaat gcctgacatg
    10621 gatggcattc gggcctgcga gctcatccac cagctccccc atcagcaaca aacgccggtt
    10681 atcgcggtaa cggcgcatgc aatggccggg caaaaagaga agctgcttgg cgcagggatg
    10741 agcgattatc tggcgaaacc gattgaagaa gagcgattgc ataatttgtt gttgcgctac
    10801 aagcctggca gcggtatttc ctctcgcgtc gtgacgcccg aagtcaacga aattgtggtg
    10861 aacccgaatg cgaccctcga ctggcaactg gcactacgcc aggcagcagg aaaaaccgat
    10921 ttagcgcgcg atatgctgca aatgttactc gatttcctgc ctgaagttcg caacaaagtt
    10981 gaggaacagc tggttggaga aaacccggaa ggcctggttg atttgattca taaactgcat
    11041 ggcagttgcg gctatagcgg tgtgccgcgt atgaagaatc tctgccaact gatcgaacaa
    11101 cagctacgta gcggtactaa agaagaagat ttggagccgg agctgctgga actgttggac
    11161 gagatggata atgtagcgcg cgaagccagc aaaattctcg ggtaacggac gtcttgtcga
    11221 gtagcaatcg gctttttatg acgctggttt cagattggtg acaaagtgcc tgtgtttatg
    11281 ccggatgcgg cgttaacgcc ttatccggcc tacaaaacca attaaattca atgaattaaa
    11341 aaatgatgta gacctgatac atagcacaac aggctatttt gcgtttagca tcagtctcaa
    11401 gccggctcca gatagagccg gttttggttt tctgtcttaa cgcaccatgc acgggcgctt
    11461 gttatcgaac gtccagccag gaatcagata ctgcattccc atcgcatcgt cacgcgcgcc
    11521 aagcccgtgt ttatgataca gctcatgggc tttcatcact tgatccatat cgatttctac
    11581 acccagcccc ggtttttccg gcacctgaac cagaccgcct ttgatctcaa acggttcttt
    11641 ggtcaggcgc tgattacctt cctgccagat ccagtgcgta tcaatagcag taattttacc
    11701 cggtgcagcg gcggcaacat gggtaaacat cgccagggaa atatcgaagt ggttgtttga
    11761 gtgtgaaccc caggtcaggc caaattcatg gcacatttgc gccacacgta ccgaaccttg
    11821 catcgtccag aaatgcggat ccgccagcgg gatatcaacg gattgcaggg agagcgtatg
    11881 gcccatctgc cgccagtcgg tggcgatcat attggttgcg gtcggcaggc cagttgcgcg
    11941 acggaactct gccatcactt cacgcccgga gaaaccttgc tccgcaccac acggatcttc
    12001 tgcataagcc agcgaccctt tcaggtattt accaatttta atcgcttcgt tcagcgacca
    12061 ggcaccgtta ggatcgagcg taatacgcgc ctgcgggaag cgttgcgcca gtgccacaat
    12121 agactcggcc tcttcttccc cggccagtac accgcctttc agtttgaaat cgttgaagcc
    12181 atatttttca tacgccgctt ccgccaggcg caccaccgca tccggcgtca tcgcttcttc
    12241 atgacgcagg cgataccagt cgcatgagtc atccggctgg ctttgatacg gcagcggtgt
    12301 ggctttgcga tcaccgacga agaacagata accgagcatt tcgacttcgc tacgctgttg
    12361 accatcgccc agcagcgatg ccacgtttac ccccagatgc tgccccagca gatccagcat
    12421 tgccgcttct atcccggtaa ctacatgaat agtggtacgc aaatcaaatg tctgcaaacc
    12481 gcgcccgccg gcatcacgat cggcaaatgt attacgcacc agcgtcagaa cgtttttgta
    12541 ttcacccagc gttctaccca ccaccagcgg aatcgcatct tccagcgttt tacggatttt
    12601 ctcgccgccg ggaatttcgc ctacgccggt gtgaccggaa ttatctttga taatcacaat
    12661 attacgcgtg aagaacggtg cgtgtgcacc actcagattc atcagcatac tgtcatgacc
    12721 cgccaccggg atgacctgca tttcagtaac aacaggcgtc gtaaattgag aactcatatt
    12781 tatgtcctta ttcagaatca gtgacggccg aaaacgggac gtttacggtc aaaggtccag
    12841 ccggggatca ggtactgcat cggacctgcg tcgttacgcg caccgccagg cagacgttta
    12901 taggcctcat gtgccttttg tacctgttcc cagtccagtt ccacgcccag accgggcgca
    12961 tcaggaacgg caatttttcc gtttttaatc tccagcggat ttttggtcag gcgacaatcg
    13021 ccctcctgcc aaatccagtg ggtatcgata gcggtaggat tacccggtgc cgccgcgccc
    13081 acatgggtaa acatcgccag agaaatatcg aaatggttat tagaatggca gccccaggtt
    13141 agcccccagt cgtcgcaaag ctgtgccacg cggactgcac cggaaagcgt ccagaagtgc
    13201 ggatcggcaa gtggaatatc taccgcattg agcatcaccg catggcccat ttcgcgccag
    13261 ttggtggcga tcatgttagt cgcgacgggc aagccggtcg cccgtcgaaa ctccgccatc
    13321 acttcacgac cggagaaacc ctgctcagcg ccgcacggat cttcggcata ggtaagaaca
    13381 tcattcagcc ctttgcacaa agaaatggct tcatcaagca gccatgcacc gttgggatca
    13441 acggtaatcc gcgcatccgg gaagcgtttc ttcaatgcac gaacagtgtc gatttcttgc
    13501 tcgccaggta acactccgcc cttaagtttg aaatctttaa agccatagcg atcctgtgag
    13561 gcttccgcca gacgcacaac ggcttcgctg ttcatcgctt tctgatggcg caactgatac
    13621 cactcatggt tgcccggcgt attttccaga taaggaagat cggttttggt ccgatcaccg
    13681 atataaaaca gataaccgag gacggtaata gcatcgcgtt gcttgcctgg ccctaacagt
    13741 tcgcagaccg gaacattcaa cgccttacct agcaggtcaa gcaaggcggc ttccagcgcc
    13801 gccacggcat taacgcgcaa ttcaaaagtc caggcacctt tgccgaaggt atcaaaatcg
    13861 gccgcctgat tacctatatg cacctgctga accactttat tcaggcgcgc aacttcctgg
    13921 cccagcacca tcggaatagc atcgacaagc gtctgataaa tcacctctcc gcccggcgct
    13981 tcaccaatac cggtatgccc ggcattatcg gtgagtacca caatattgcg ggtgaaatat
    14041 gcgctatgtg caccaccaat attaagcaac atgctatcgt gcccggcgac cggaatgact
    14101 ttcatatcag taataacagg actggattgt gtcgtcatct tattgccccg cgacaggttt
    14161 cagctcgata cgcttgatat cgcccaccag caccaggtag ctcagtaccg cgattaaggc
    14221 atgaacacca acataaatca gcgccccatt aaaggagcca gtcgtgccaa cgatataacc
    14281 aattgcgatt ggcgtgacga tgccagaaat gttaccgaac atgttgaaca ggccaccgga
    14341 aagaccgctg atctcttttg gcgcggtatc tgccattact gcccaaccca gcgccccgat
    14401 gcccttaccg aagaaggcca gcgccataaa gccgatgatc atccactcaa cgttgacgta
    14461 gttgcagaac accatcacca tcgacagcaa catgcccatt acgatcggtg ttttacgcgc
    14521 aatgttcagc gatcccgtgc ggcgcatcag ccagtcggaa ataatcccac ccagcacacc
    14581 gccgataaaa ctgcaaaccg ccggaacgga agccacaaag cccgctttca gaatcgacat
    14641 cccgcgtgcc tgcaccagat aaaccgggaa ccaggtaata aagaagtaag tcagggcgtt
    14701 gatacagtac tgaccgatat aaacgccgat catcatccgt gacccgagca gctgtttgat
    14761 ctgcccccac ttcacgctga acggcacttt aactttggtg ttttgctgat ccatattgat
    14821 cagcgcgcca cccgcggcga tgtactccag ctctttctga tttacccccg gatgttgatt
    14881 tggctcgtgg atgactttca accagataaa gctgatgaca atccccagac cgcccataaa
    14941 gaagaagacg tgtgaccagc ccacttcatg cgtcagccag cccataatcg gcgcgaagat
    15001 caccgttgcg aagtattgag cggagttaaa aatcgacacc gccgttcccc tttcctgcgc
    15061 cggaaaccag gccgcaacaa tgcgactgtt gccggggaaa gatggcgctt cagcaagccc
    15121 gaccaggaag cgcagagtaa acagggcaac gataatgccg aatccactaa agatatcgac
    15181 gaagccttgc agcaaggtaa acatcgacca gataaagatc gaccagaagt agacgcgttt
    15241 tgaaccaaaa cgatccagca accaaccacc agggatctgc ccgataacat aagcccatga
    15301 gaaggcagag aacacatagc ccattcccac gggatcaagg ccgatatctt tggccatttc
    15361 cgaaccggca atagaaagcg tagcgcggtc gccgtagttg aaggatgtga cgataaacaa
    15421 catcaccact atccagtaac gagcattcgt gcgtttttcc acactgctcg cagcctgact
    15481 taaagaactc attgttgcac tcctgaaaat tcgcgttagc cacgctcact ctggactgcg
    15541 acatagccag gaaatcagag gtgacgtagg gtgttttttg ccgtttttat aggtcgttcg
    15601 ccgaatacgg cgcgtgttta tatctggcaa tagcagtata aaaagcgcgc catagcggct
    15661 caccgtgcaa caacacaaca ttaatgcgtt caatgaggcc cgattttggc attagccctg
    15721 gacggtggaa tccacttcac ggaaatgaaa acaagaacaa gaaaggaagg gttaaaacga
    15781 agaaataaag aagagtatga aatggatcgc ttgactccag gcaaacgcca gtaaaaatcc
    15841 gcgctatgaa gcagttttta ctggcatttg cctgaaaata ttcgattcag caccgctaaa
    15901 acgacattta ccgctcgctg aacatatcag gacaacagcg tgccccactg ttcgacccac
    15961 ggatttgatt ccgtttccgg ttccgggttc tcactggcgt caatcaacag catttcgcca
    16021 acccgctgag cgctctgttc ctgcaacaag gcatcgaact gtttgccgcc attgcagaaa
    16081 tttacataac tgctgtcgcc gagcgcaatc acgccataac gcagattcgg ctggaagccc
    16141 agactatctt tgattccctg aaagagcggc acaatgctat caggaaggtc gccctgcccg
    16201 gtcgtggacg taaccaccag aacatactta tcctgatagg gcagccagtc gcttaattca
    16261 ggatcttcaa ataccgttgc tttgtggccc tgtgcggtca gaattgcttc tgcttcttcc
    16321 gccactaaca gcgaatttcc gtacatggtg ccgacaaaaa taccaatttc cgccataccg
    16381 ttttctccct ggattaggaa cttatctctc catcctgacc cgatggcgca ctgaactcaa
    16441 ccctttcatt ttcaggaagc agaccgcgcc agccaaattg tgataacgcc tgcatccagg
    16501 tgtcgtccag gcccgcgtga atagtcagcg gctcgccagt aaaaggatgc gtcaatgaca
    16561 actgactggc atgtagcatt aaccgctgga ggccaaaatg ctcagcaccg ctgcgattct
    16621 ggcgtaaatc gccatgtttg ctatcgccaa taatcggatg acgcaaatgg gcaagatgtc
    16681 ggcgaagctg atgtttgcgt ccggttttcg gctccagttc caccaggccg tagcgcgtgg
    16741 tcgggtaacg tccggtcgct accggcattt ctacggtcgc cagaccgcga taatgcgtca
    16801 ctgctggctg cgagccttta tcttcgcggg caaatttatc agcgattttg tccagttctt
    16861 ccaccagtgg ataatccaga actgcttctt ccatcaacca gccgcgcaca atcgcatggt
    16921 aacgtttctg gatttggtgc tgttcaaact gttgtgccag cagccgtccg gcctcgctgg
    16981 ataatcccat caacaacaca ccagaagtgg gtcggtccag acgatgagcg gtaaaaacgt
    17041 gctggccaat ctgatcacgc acggtttgca tgaccactac tttctcgtcg cgatccagcc
    17101 agctgcggtg aaccagccag ccggagggtt tatttaccgc aaccagccat tcatcctgat
    17161 agagtatttc cagcattagc tcgcatcatc cgcaaaaaga gcatccagtt tttccagctc
    17221 agccagaata agcgcgcgtt gcggatggtc cgtcgccagc gccatttcat aatagggtgc
    17281 aacggcaaaa gcgcccggta acggctgttt gttatctaac aaatcgtgca ttcgcgggat
    17341 cagcacccac tgcaaccact ccagtggttc catggtgtcc ataaagaacg gttgggtact
    17401 attaaattga tgcggctggg gttcatcgtt tcgccagtgc tgatgttcac gcagtaacgc
    17461 ttcaagcgcc tgcaactgga gacgaacgcg gtcatgagtg gtcataaagc aacctcaata
    17521 agaaaaactg ccgcgaagga tagcaggaat aaaaaaaggg agcactgtat tcacagcgct
    17581 cccggttcgt ttcgcaacat tccagctact tttgttgctc cctgctcatc cttgacaact
    17641 tttcctctgg ccttgcggcc aatcgttcat cctgaactat tgcttcctgc tcacaccacc
    17701 ccgatgtgat acttcatcct gaagtgtccc tggccttcct gacccaccga atcatcctga
    17761 ccggttctca ttctccatcc tggaggtgtc ctttaacgcg tcctgcgtca tcctcttcgc
    17821 ttcatccaga agcctttccc tgaaacacca tcctggtgtg tcctgcagaa gtgtcatcat
    17881 cctgatgttc acttcgttgt ctgactccct gtcgacgaag atagaatcgt ctttttcgcg
    17941 aagtcttaca aggcgcttac agacaatgcc ttaaggaaaa tttcatatga aagttaagaa
    18001 ttaacaatca acattttgat tttaaatgaa aataacgatt cttatctttt taagtattcg
    18061 cttatttcct ggcgatctcc tacagagtct gtaagagatc tctcacaacc agatggggta
    18121 tttggattac agaagaggct taagttgatt aaggaattcc gcaagatttg aggctaaatg
    18181 cgtccgttta cgtgttccca gcgtctcttt gcacacttca ccggagagat tacacaccga
    18241 gatgacctct aactcttctt ccagcgtcgc gataaatagc gtgggcggca gttttagcct
    18301 tttctgagtc accagatgac caatcagatt ttcctgcacc cgacggaagt cgtcttcact
    18361 ccaggtttgc agcaatgtca gtttgatatc gccaaactgc gcgtgcatat ccccggcaaa
    18421 ctgagtcgta taaaaggtat gaattgtggg ttgtatcaca atatcaaaag cgcgttcaac
    18481 cgcgtttaca ttttgttccc cggtaaacgg ctggggttgc cagtatacgg catcttcggt
    18541 agttgaaata atgcacggtg aaggaacacc gtacagttcc tcacttaacg gccagctttt
    18601 atgctcttca tgccacgcat cgcagtagcg tgcagtaaaa tctttcaggg cttgtgcggt
    18661 caaatcgtcc actaatttct ctcttcactt aaaccagata cacttgtcgt ttagtttatc
    18721 tggtttatga cggtgaaaca tgtcttctta tgcaaaccat caggcacttg cgggcctgac
    18781 tcttggaaaa tcaaccgatt accgggatac ctatgacgcc aacctactgc aaggcgttcc
    18841 acgcagcctg aatcgcgacc cgctgggtct gaaagcggat aacctgcctt ttcacggtac
    18901 ggatatctgg acgctgtatg aactttcctg gctgaatgcg aaaggtttgc cgcaggttgc
    18961 cgtcggtcac gttgaacttg attacaccag cgcaaatctg attgagtcga agagttttaa
    19021 gctctatctc aacagtttta accagacgcg ttttaataac tgggatgagg tgcgccagac
    19081 gctggagcgc gacttaagca cttgcgccca gggtaaggtt agcgtggcgt tatatcgtct
    19141 tgatgaactg gaaggccagc cgataggtca tttcaatggc acctgcattg atgaccagga
    19201 tatcactatc gataactatg aattcactac tgactatctg gagaatgcca ccagcggtga
    19261 aaaagtagtg gaagagacgc ttgtcagcca cctgctgaaa tcaaactgcc tgatcaccca
    19321 tcaaccagat tggggttcga tccaaattca gtatcgtgga cgccaaattg acagagaaaa
    19381 actgctgcgt tacctggtct cattccgtca tcacaacgag ttccacgaac agtgcgtgga
    19441 acgcatcttt aatgacctgt tacgcttctg ccagccagag aaattaagcg tttacgcacg
    19501 ttatacccgt cgtggcggtc tggacattaa cccgtggcgc agtaatagcg attttgtccc
    19561 atcgaccaca agactggttc ggcaataaat tttttctcaa ttttgcgtgc tggattcacg
    19621 cagaaggttg tgaaaggtca tcaggcaggg ctattgtaat caaagggaat gacgatattc
    19681 gtcccataag gagtttttct tgattacaca tattagcccg cttggctcca tggatatgtt
    19741 gtcgcagctg gaagtggata tgcttaaacg caccgccagc agcgacctct atcaactgtt
    19801 tcgcaactgt tcacttgccg tactgaactc cggtagtttg accgataaca gcaaagaatt
    19861 gctgtctcgt tttgaaaatt tcgatattaa cgtcctgcgc cgtgaacgcg gcgtaaagct
    19921 ggaactgatt aatcccccgg aagaggcttt tgtcgatggg cgaattattc gcgctttgca
    19981 ggccaacttg ttcgcggttc tgcgagacat tctcttcgtt tacgggcaaa tccataatac
    20041 cgttcgtttt cccaacctga atctcgacaa ctccgtccac atcactaacc tggtcttttc
    20101 catcttgcgt aacgctcgcg cgctgcatgt gggtgaagcg ccaaatatgg tggtctgctg
    20161 gggcggtcac tcaattaacg aaaacgagta tttgtatgcc cgtcgcgtcg gaaaccagct
    20221 gggcctgcgt gagctgaata tctgcaccgg ctgtggtccg ggagcgatgg aagcgccgat
    20281 gaaaggtgct gcggtcggac acgcgcagca gcgttacaaa gacagtcgtt ttattggtat
    20341 gacagagccg tcgattatcg ccgctgaacc gcctaacccg ctggtcaacg aattgatcat
    20401 catgccagat atcgaaaaac gtctggaagc gtttgtccgt atcgctcacg gtatcattat
    20461 cttccctggc ggtgtgggta cggcagaaga gttgctgtat ttgctgggaa ttttaatgaa
    20521 cccggccaac aaagatcagg ttttaccatt gatcctcacc ggcccgaaag agagcgccga
    20581 ctacttccgc gtactggacg agtttgtcgt acatacgctg ggtgaaaacg cgcgccgcca
    20641 ttaccgcatc atcattgatg acgccgctga agtcgctcgt cagatgaaaa aatcgatgcc
    20701 gctggtgaaa gaaaatcgcc gtgatacagg cgatgcctac agctttaact ggtcaatgcg
    20761 cattgcgcca gatttgcaaa tgccgtttga gccgtctcac gagaatatgg ctaatctgaa
    20821 gctttacccg gatcaacctg ttgaagtgct ggctgccgac ctgcgccgtg cgttctccgg
    20881 tattgtggcg ggtaacgtaa aagaagtcgg tattcgcgcc attgaagagt ttggtcctta
    20941 caaaatcaac ggcgataaag agattatgcg tcgtatggac gacctgctac agggttttgt
    21001 tgcccagcat cgtatgaagt tgccaggctc agcctacatc ccttgctacg aaatctgcac
    21061 gtaatctccc ctcccccggt gacgtttgtt gccgggtttt ctcgtttttg gtcacttact
    21121 catcaactca tttcatttgt tatatgaatg tttcttacca ccctcacgcg acaaatatca
    21181 tcatagttaa tatgtcatac aatttatatt gcaacgcaaa cgtttcccta ttttcataaa
    21241 ccgtatttta tcgctatgaa aaagaaatta tcgccatgat taactaaaag tattgatttt
    21301 ttcagttcaa cctacatata ttgcgcgccc cgga
//
LOCUS       DAZFQD010000086        20880 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.086, whole genome shotgun sequence.
ACCESSION   DAZFQD010000086 DAZFQD010000000
VERSION     DAZFQD010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20880
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.086"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(184..324)
                     /locus_tag="ACHKTW_003198"
     CDS             complement(184..324)
                     /locus_tag="ACHKTW_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO6464169.1"
                     /translation="MPQKTIIVSILCLTMLLAIWMIHRSPCEFRLNIMWSEFAAFLQC
                     KQ"
     gene            complement(459..761)
                     /locus_tag="ACHKTW_003199"
     CDS             complement(459..761)
                     /locus_tag="ACHKTW_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001299564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ogr/Delta-like zinc finger family protein"
                     /protein_id="HFO6464170.1"
                     /translation="MFHCPFCGASARIRTSKAANDTGTIRYKYYQCNNLECSATFSTM
                     ESVHRIIGQPQLIFHDKDIPRETMRKSHQGRSQLTLQLEDCGMALKPVHYARVPFG"
     gene            complement(806..1930)
                     /locus_tag="ACHKTW_003200"
     CDS             complement(806..1930)
                     /locus_tag="ACHKTW_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage late control D family protein"
                     /protein_id="HFO6464171.1"
                     /translation="MVLLNGNTVYSPLKELVKMPDFSVSLDREQDKNTLQELNNRVIS
                     LSMTDNRGFEADRVTLVLDDADGQLALPGRGTHIRVMLGWKGEPLVDKGTYIVDEVAH
                     EGPPERLTLSANSADFREEFNVKREVSWHDVTVERVVSAIAHRYGLKAQISDMLMDIE
                     IDHADQTEESDISFLTRMADMLGAITTVKNGCLLFILPGGGVTADGRPLPSFALARSD
                     GDSHQFRIADRQAYTGVKAYWLDLNHGKKKAVNIKKRKTHAEKSSSREGSYMEGAEGN
                     VFVLRKTYPNEIAAQRAAAARWQQLQRGAAEFSITLARGRAELYPEMHGTVTGFKTEI
                     DNQDWLIAKVEHNIDGSGFTTRLELEARIPEWIAEKESNG"
     gene            2085..3272
                     /locus_tag="ACHKTW_003201"
     CDS             2085..3272
                     /locus_tag="ACHKTW_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HFO6464172.1"
                     /translation="MAETRFHGVRVRENADLVTAINDIDSSVIGIVAVADDADAKTFP
                     LDKPVLLTRVMDVLGKAGKTGTLYKSLKAIADQVSTRVVVVRVAAAKEGDGQPTQTQL
                     VIGEVKNDGTYTGMQALLVAEQNEAVGVRPRILAVPSLDTKEVVAALVTVAKKLRAFV
                     YAGCPGCNTVQEAVTWRADLAHREVMLIWPDFIAYNPNSGQNEAFPASAYACGLRAKI
                     DHEQGWHKSLSNVAVNNVLGISVPVFWSLQAEDSDAETLNDKEITTLIKRNGFRFWGN
                     RTLDTTTYTFETYTRTAQSLADTIAESQFETVDKPLTPSNVKDVLSDIRAKLSALVTA
                     GKLIGAECWYDVVDNGTTDLRQGIVRIRYRYTPVPPLEDLELHQTFTDEFFDGAFASL
                     GGE"
     gene            3272..3781
                     /locus_tag="ACHKTW_003202"
     CDS             3272..3781
                     /locus_tag="ACHKTW_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail tube protein"
                     /protein_id="HFO6464173.1"
                     /translation="MAVPNKLRVFTVYIDGENKLGRVTSFTPPKLTRKTESFRGAGMP
                     GSVSVDFGLDDGALDLSFAVGGADAHLFSRYAGSLDEVRLRFSGEYYSDADIRYVDIE
                     VRGRITEIDMGEAKQGEDTEHSYSMKNTWYRLSVDDREKIEFDVLNFIWRIDGKDVLP
                     DRTRSMLGF"
     gene            3829..4218
                     /locus_tag="ACHKTW_003203"
     CDS             3829..4218
                     /locus_tag="ACHKTW_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6464174.1"
                     /translation="MDKETEMTTDTAVSNGEASAVAVAATAAPKNVVKLTVPVVRGDV
                     SYSEIKIGPLANQAGSLRGLSLLALYNMNCDAMVTWLARVTEPKLKEAEMYAMNTADF
                     TALSSAAVNFFAPSGSNAPAPENTAEP"
     gene            4239..4376
                     /locus_tag="ACHKTW_003204"
     CDS             4239..4376
                     /locus_tag="ACHKTW_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GpE family phage tail protein"
                     /protein_id="HFO6464175.1"
                     /translation="MEDPVADIAVIFNWPPAEIFAMTPAEVVAWRERAAARSGSHNHD
                     E"
     gene            4366..7158
                     /locus_tag="ACHKTW_003205"
     CDS             4366..7158
                     /locus_tag="ACHKTW_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO6464176.1"
                     /translation="MMNNLHIRVALAAADTLTQPLRRARETVGALSDSLRETQDSIRS
                     LERQSEAFAAAQTKFDEATRKIAAVQQQLNRLQQAEREGTVLSERQREMMASLTTRLE
                     RLNRVRDVHQRRLEDSRRELERHGIAAEGSSQTIQAAIRRTAEYNEELARQRQALAQV
                     TRAQQRYEQAQERLGRIRNAGAAALAAGAAGLYAGGRAVAPVMDLQRHGAVIAAQAGE
                     GASDAVGYTGVINHMVHTGITPDAQLAAEAVGAVRSTLGGLGAVSENELTRITRKMLD
                     MQAVMGGDIPQHIQAAAIMIKNGLARSSDEAVDLMVAGMQRMGPVMRDELPEILHEYS
                     THFRNMGLTGSETMTLLADMAQQGKFALDKTGDAIKEFSIRGSDMSKASAEAYALIGL
                     DAARMSSAVASGGTAARAALQKTASGLLQIVDPAERANAAIALFGTPVEDLSVDQIPA
                     FLGALARVSDRMADVSGVADRMGQTLRDNLSGDVARLTGTFSALRSGIIGEITDDLRG
                     LVRMATAWGERLQAWVNAHPELVRMIALTTGAVLALTTAMGALSVAAWMVVAPLLKLR
                     LGLALLRAIPAGLSVAGTLFGGLTGVISAVGAALGALTWPVVVAVGALVGGALLIWRY
                     WDRVRAFVSGFIEGVQSALAPFMATASRFAPLLDLVSGALSRILGWFKSLLTPVSSTR
                     DLLEQCTSAGQTFGSTLAGALATVFSPLKALIDGLAWVLEKLGVLPGEAERVKRKLDE
                     ARKTPVAWEWDPEQKKMVQKAWHWSPPGGASVPSGASGTQPPPVALPAGIAPDGNRNV
                     LRHLKGIEGNTGGLLAENRKRIGPGDIVFKHLPRALAVRGQWHEPVVAPSSGGMRALP
                     QEEIQVQQWPTARPAPRMHQQETFASAFQGEIHVHLHGVSSHNPRELARMVGDAVRAE
                     MNRMARAGTGSFRDRD"
     gene            7169..7660
                     /locus_tag="ACHKTW_003206"
     CDS             7169..7660
                     /locus_tag="ACHKTW_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO6464177.1"
                     /translation="MMMIYGMFVFQLSTLPHQQVEQSKRWRHVSNERVNRSSSWQYIG
                     SGEDHITMSGVLCPEITGGEVSLTALNTLAYTGLPWPLIDGVGQIYGMYVITEIRTTR
                     QELDRYGMARKIEFTVSFQRCNEDLRERLQSGSVGDLMAGLKNSASSALSSASSALSG
                     LML"
     gene            complement(7671..8258)
                     /locus_tag="ACHKTW_003207"
     CDS             complement(7671..8258)
                     /locus_tag="ACHKTW_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HFO6464178.1"
                     /translation="MHTIKSARNAAYNRDGTIDCEIQFEGEESWLPYTATEYDNSPHG
                     KKLWGELKAGNHGPVSEYQENDTDRENARRLKNDEINAWRNAMEAASYTFEHNGRKWD
                     YGKSTQARLEPSVAAAKAGILPLDFFWTDAQNNDVPVSADELIALSDAAAKALFAKGM
                     EIHIRQRNMKKEIAALNDTAAILDYKVGWGKEAEA"
     gene            complement(8270..8701)
                     /locus_tag="ACHKTW_003208"
                     /pseudo
     CDS             complement(8270..8701)
                     /locus_tag="ACHKTW_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764700.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
     gene            complement(9008..9610)
                     /locus_tag="ACHKTW_003209"
                     /pseudo
     CDS             complement(9008..9610)
                     /locus_tag="ACHKTW_003209"
                     /inference="COORDINATES: protein motif:HMM:NF023985.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
     gene            complement(9610..10233)
                     /locus_tag="ACHKTW_003210"
     CDS             complement(9610..10233)
                     /locus_tag="ACHKTW_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein I"
                     /protein_id="HFO6464179.1"
                     /translation="MNNRLLPPSSPSFLHHSEQGAQRLSELPIHINTLWNPDTCPAHL
                     LPWLAWALSVDRWDETWSEEARRQIIRDSWHIHRHKGTISAMRRAVEPFGAILSITEW
                     WENNDPPGSFRLDIDISERGLTEETRQEMERMITTVRPVSRHLAAMKMTYVARGVSRI
                     TGATLTGETIVVYPAAELTSPLSVSGDIHPGGVTYEGITVTIRPDKE"
     gene            complement(10226..11122)
                     /locus_tag="ACHKTW_003211"
     CDS             complement(10226..11122)
                     /locus_tag="ACHKTW_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HFO6464180.1"
                     /translation="MPTIDLSQLPRPQIIESLDYESILKDVKAFMIDAFPENMRSAVS
                     AAIALESEPLNIIAQAIAFREMLLRQRINDAAAGCMLSHAVSTDLDNLAAIVNAKRLE
                     LKPATDTEAAIMESDSAFRLRAQSGFEGFSVAGPSAAYEYFARSASGLVADARATSPS
                     PATVIVSILSTEGDGTASPELIATVSRALNDETVRPLADRLTVQSATIVSYDIEAVLY
                     FYPGPESEPILAAARDALDTWLKTQNAIGRDVARSAIMATLHVPGVQRVELKKPAQNV
                     VISNTQSARCTSRNISAGGTDE"
     gene            complement(11109..11474)
                     /locus_tag="ACHKTW_003212"
     CDS             complement(11109..11474)
                     /locus_tag="ACHKTW_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HFO6464181.1"
                     /translation="MTPVCTGMNPHGSGCISDTEHLRHSVNNILTTPRGSRIMRREYG
                     SLLPELLDAPQNDVTRLQCMSAVVMALAQWEPRIAINRVDIAWLPDGRALLAISGVLT
                     RTMQPVQMSIPLRGSNADN"
     gene            complement(11471..12052)
                     /locus_tag="ACHKTW_003213"
     CDS             complement(11471..12052)
                     /locus_tag="ACHKTW_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009486481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HFO6464182.1"
                     /translation="MNAEIMRLLENMVRTGVVSEVDLKNWRIRITSGGLTTDWLRWNC
                     TRAGAFKIWLPPSPGEQVLLLCPGGSPEMAVVAGSLYSEEHPAPGTKQNEVILLAPDG
                     AEIRYDAEKGALTVKGIKTAEISAETRITLDTPAVECTKHLKARTFELTDGGTMKGDI
                     THSNGNLRSNGVTVHTHVHNGVQSGGSMTGGPK"
     gene            complement(12049..12693)
                     /locus_tag="ACHKTW_003214"
     CDS             complement(12049..12693)
                     /locus_tag="ACHKTW_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000968270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HFO6464183.1"
                     /translation="MMAPDDLHRLDGLFQEILSGLSAAGINKTARAVGQAVRAAQQKR
                     IRSQQSPDGNRWPARKKRVFRVQAGVGFIWNGTQVRRLKNWRTTTGRYGPMITGYDTE
                     RRGIRSFHKADIEEYLEIDTRRRTQSAIKRPPMFEQLRTNRFLKVKTDPGGVSVGFEG
                     RAARIARVHQFGEESLVSAGNAVRYPQRELLGFSEADRQKVTEIIINNLWRNTR"
     gene            complement(12677..13144)
                     /locus_tag="ACHKTW_003215"
     CDS             complement(12677..13144)
                     /locus_tag="ACHKTW_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO6464184.1"
                     /translation="MLKINTLRDAMTDACPWCRANPEKFTVYVNNGGIETTGESPSYL
                     YRYTLGIFVMDFTQDLDELMVPLMRWLYRNQPDLLLNPEKNRDIKFSVAINDDDSTDI
                     LLEIPVWERVIIRRNDDGTVHAEHIGEPPVSRALEAWRVTLEDVTWGQNDGSG"
     gene            complement(13160..13333)
                     /locus_tag="ACHKTW_003216"
     CDS             complement(13160..13333)
                     /locus_tag="ACHKTW_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HFO6464185.1"
                     /translation="MLFIHQIMLYFCTAVCALYLISGGYSVIRNYFQRKIDERAAEKI
                     SQKSAQQNNQNMP"
     gene            complement(13480..15525)
                     /locus_tag="ACHKTW_003217"
     CDS             complement(13480..15525)
                     /locus_tag="ACHKTW_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFO6464186.1"
                     /translation="MVPKHYQLVTAMTNKELAEKLAPMLREGWGLLGGPVSASEPGAP
                     FYLMQALVTDRDLPSAIPVPKGEENPVPGTTDGYDTGHTTEPEFYYVVPLAGQSNGMA
                     YGEGLPLPDTFDSPDPRIKQLARRSTVTPGGAACTYNDIIPADHCLHDVQDMSSLNHP
                     NADLSKGQYGCVGQGLHIAKKLLAFIPKNAGILLVPCCRGGSALTSGAEGAFSESSGA
                     TAESSLWGVGKPLYNDLLTRTKAALDKNPKNVLLSVVWMLGEFDLKSATYAQHPQLFL
                     NMVNQFRADLSAYAAQCTGGNASLVPWICGDTTYIWKQQYTSQYPVVYGAYQNKEAQK
                     IYFVPLMTDEKGANTPTNLPAEDPDLAEAGYYGAASRSNGNWTSSDRATHFSSWARRG
                     IISDRLATAILMHAGKTVAFLKGAPAPFSVVAAPPATAGSDSTTTESPQPSTPAPQPG
                     AGTQSSPARTTAVAYRADAQEGKLKEQGWTGADGKASVVNDSAAQSGKAVRVEKVQGQ
                     QTAWKIAHDVPASKATELLQKGGEIAFRFKIPEGVQLAENRFAMALYWPVSQLPENVV
                     PEGSTGNNMLAAFFIQSDSANLNLMQHKQQNAKLGTFGAFNHNWHNVVFRFAGNNSIS
                     VTPVINGQTQAAFNLAKCPAAGLDNDKLLITDITSNKDTYPVLIEYVTVDINNSNAA"
     gene            complement(15536..15667)
                     /locus_tag="ACHKTW_003218"
     CDS             complement(15536..15667)
                     /locus_tag="ACHKTW_003218"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6464187.1"
                     /translation="MIWADVADWGDRLVDALDSCNADKAAIAGLERQRTARMNTHEK"
     gene            complement(15654..16079)
                     /locus_tag="ACHKTW_003219"
     CDS             complement(15654..16079)
                     /locus_tag="ACHKTW_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000773182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2570 domain-containing protein"
                     /protein_id="HFO6464188.1"
                     /translation="MKLNIILCALLLTISLQAMVVIRAYRSGQESTRYEALEKAVKES
                     NTTVRELLKATAETQSALAAIRREDQERAARGEERRETMRKNLQADECARTSPPADVA
                     DSLQQHASAAENRRHLRERAGKSDQTDTGTGAAATHDMG"
     gene            complement(16076..16618)
                     /locus_tag="ACHKTW_003220"
     CDS             complement(16076..16618)
                     /locus_tag="ACHKTW_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO6464189.1"
                     /translation="MAGPKRYAVAAIVALGISLAPGELRTSREAQIKIATWEECRARP
                     YRDLVGVGTVGCGSTGNVENRLYTQEEVAGRWINDMRRAENCITQNFRGQQMPQSAFE
                     AMTDAAFNLGCRNLMWFKNKNGTPQRTTIWKHAQTRQWRLMCYRLTDFVNSGGTYTQG
                     LVNRRNDFKNWCLKDAGAQP"
     gene            complement(16605..16889)
                     /locus_tag="ACHKTW_003221"
     CDS             complement(16605..16889)
                     /locus_tag="ACHKTW_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO6464190.1"
                     /translation="MFQRLCDHITFWASVTTAGIGVMTLSEKIAVAGFVLGTLSLLRA
                     WLHRRRMEIAQARRNELIEKILMQSEGRELNDAERRAMMLLKQDEHDGRT"
     gene            complement(16892..17092)
                     /locus_tag="ACHKTW_003222"
     CDS             complement(16892..17092)
                     /locus_tag="ACHKTW_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000866986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail protein X"
                     /protein_id="HFO6464191.1"
                     /translation="MKVMALDGDTVDLLCQRHYGTTQGVTEAVLAANQGISAVMFLHA
                     GQEVELPELQGQTRREMIQLWG"
     gene            complement(17092..17586)
                     /locus_tag="ACHKTW_003223"
     CDS             complement(17092..17586)
                     /locus_tag="ACHKTW_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000052292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head completion/stabilization protein"
                     /protein_id="HFO6464192.1"
                     /translation="MSLIARPRVNSADTDTTDTDDGDAMVSAGDFWPAISLHEIRLAS
                     RIPGRTTTTRLLHAATEAVAHVIAELELWKRERMAEGAASLADVPATQVNGESVHVHR
                     YRRAVFALTRAFVLERTRDVDTTDKGDRRADALDMQVEDLWRDARWAISDIRGVTRIY
                     AELV"
     gene            complement(17691..18488)
                     /gene="gpM"
                     /locus_tag="ACHKTW_003224"
     CDS             complement(17691..18488)
                     /gene="gpM"
                     /locus_tag="ACHKTW_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit"
                     /protein_id="HFO6464193.1"
                     /translation="MLTPAQRYVQQAVARNRVTMNARETQRERTAHEQVLHQLRLAQM
                     QLKGIQSNVARAELKKHLLPDFEGWIEGTLAGDSGRQDEVITTMMVWAIDCRDYPLAL
                     RIGEYVIRHGLALPDNFGRDAATVLAEEITDAVLTQATTDSEADLSVYTAMLDELNHL
                     VSEQDMPDQVRSKLHKTRAFSRRASSSPDDLRLSLSLLREAMRINPASGVKREIATLE
                     RAVRKLADGNQVVPASTTGRKTTTGGKKSSAKKKSAASQKKAARKTN"
     gene            complement(18529..19629)
                     /locus_tag="ACHKTW_003225"
     CDS             complement(18529..19629)
                     /locus_tag="ACHKTW_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019028 - viral capsid [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein, P2 family"
                     /protein_id="HFO6464194.1"
                     /translation="MARLSLSREGRDSLERYMIQQASLAGITTERLGKTFSVDPSIQQ
                     KMENAIKESAGLLQEINFIGVDDQEGEKVLIDTSGTIASTNATSDGVKRRNPASVADL
                     AARRYRCEQVNYDTFISYAQIDAWSSQKNFQQLLSAQITRQIALDRIMIGFNGESHAL
                     ISDRATNPLLQDVNPGWLKHIRDKAATRVVKGMTLTRRDDENKLVAKGDYGNPDAMVH
                     DIRSSVLDEWHKDAPDLVVLMGRDLFNTLRLPMINAISTTNPNTELVAGQLIVASRTV
                     GGLQTYLAPYFPKDAMLITSLKNLSIYFQKGSLRRFIREEPEYNRIATYQSMNDCYVV
                     EDYGKCALIEGITFAGEDPDNNAESGQPQPEA"
     gene            complement(19647..20483)
                     /locus_tag="ACHKTW_003226"
     CDS             complement(19647..20483)
                     /locus_tag="ACHKTW_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GPO family capsid scaffolding protein"
                     /protein_id="HFO6464195.1"
                     /translation="MGSKNTPERKKFRVAVSGSTIDGREISGEMLRQAAENYDPSVYA
                     SRVNIEHVLSMWPSSELCAVGDLIALSTDEITEGKLAGRTALYAEIEPTDRMKNMIAD
                     GKKVYSSIEMLPELDAVKGPYIVGLAMTDTPASLGTERLEFAAQQRASIMQFSKCTTE
                     PPMFTVAMEAEMLTTTEQRTEESRQWFSRVMALISKTRTADGEQFSQLRDVVENIASD
                     HADLIDRFSTLEQKHALDKTALDELSHELAALKAQLRNQDADPQQRFTSAGGTDQTLA
                     EF"
     gene            20639..>20880
                     /locus_tag="ACHKTW_003227"
     CDS             20639..>20880
                     /locus_tag="ACHKTW_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO6464196.1"
                     /translation="MIQDVFIRMRAKQLYWQGYTPADIAQLMGINQNTIYSWKKRDEW
                     DATPPIQRVTQSIDARLIQLTAKPDKSGGDFKEIDLL"
BASE COUNT         4081 a         5616 c         5963 g         5220 t
ORIGIN      
        1 cgtgtaatct actgatttcc ctttgtgcgc gcaatgctca gtgttgaaac tgtttagggg
       61 gagggcggca aaaaagcccg catattgcgg gcaaatacgt ctgtaatcca atggagaaag
      121 gggtgccata tcgactgaac ccgacaaccg gatagcgggg gctttcaccc ccgctgcggt
      181 ttcttactgt ttacactgca agaacgccgc aaattctgac cacatgatgt taagtctgaa
      241 ctcacacggc gaacggtgta tcatccagat ggccaacaac atggttaagc ataaaatgct
      301 cacgatgatc gttttttgcg gcatagcgct tgactccttt tacagggagg cgctaacctt
      361 ccacttgtct agggtaggaa tggttagggc ctcgggttaa acaatatgtt tgactcgggg
      421 cctttccaca tccggccttc aggttttcct tccagccgtc agccgaaagg cacccgcgcg
      481 taatgtactg gttttaaagc cataccgcaa tcctccagtt gtagcgttaa ctgactgcgt
      541 ccctgatggg atttgcgcat tgtctcccgt ggaatatctt tatcgtggaa gatcaactga
      601 ggctggccaa taattctgtg cacactttcc attgttgaaa atgtggcgct gcactcaagg
      661 ttattgcact gatagtattt gtagcggatg gtgccggtat cattggcggc tttactggta
      721 cggatccggg cagatgcgcc gcagaatgga caatgaaaca ttgatatacc cccctggctg
      781 aactggggtt gaactcgtcg ccattctaac cattgctttc cttttcggct atccactcgg
      841 gtattcttgc ctccagttca agccttgtgg tgaagccgct tccgtcgata ttgtgttcca
      901 cttttgcaat gagccagtcc tgattgtcta tttcggtttt aaagccggtt accgtgccat
      961 gcatttcagg gtaaagctcc gctctgcccc gggccagcgt gatggagaat tcagccgcac
     1021 cgcgctgtaa ttgctgccac ctggcggcag ctgcgcgctg tgcagcaatt tcatttgggt
     1081 aggtttttct cagaacgaac acgttacctt ctgccccctc catgtagctg ccttcccggc
     1141 tgctgctctt ttcagcgtga gtttttcgtt tttttatgtt gacggctttc tttttgccat
     1201 gattcaaatc cagccagtac gccttcaccc ctgtgtatgc ctggcgatcg gctattctga
     1261 actggtgact gtctccgtca ctgcgtgcca gtgcaaatga ggggagtggg cgcccgtcag
     1321 ctgtcacgcc gccacccggc agaatgaaca gcagacagcc atttttgacg gtagtgatgg
     1381 ctcccagcat atcagccatg cgggtaagga atgatatgtc gctttcttcc gtctggtcgg
     1441 catgatcgat ttcaatatcc ataagcatat cgcttatctg ggctttcagt ccgtaacggt
     1501 gggcaatagc ggaaaccacg cgttcaacag tcacgtcgtg ccaggatact tcccgtttga
     1561 cgttaaattc ctccctgaag tctgcgctgt ttgcactcag tgtcagccgc tccggaggtc
     1621 cttcatgagc gacctcatca acaatgtagg tgcctttatc aaccagaggt tctcccttcc
     1681 atcccagcat gactctgatg tgtgttcccc gcccggggag agccagctgc ccgtcggcat
     1741 catccagcac aagagtgacc ctgtctgctt caaacccccg gttgtctgtc atggaaagcg
     1801 atatcacccg gttattcagt tcctgaagtg tgtttttatc ctgctccctg tccagtgaaa
     1861 cgctgaaatc aggcattttc accagttctt tcagggggga ataaaccgtg ttaccgttca
     1921 gcaataccat aattcctccg tttttcccgc cacagttgca taccagaaac accaccggaa
     1981 tgagtttggg ttgtatgtgt attgcgagaa accgcagttc gtgaaaaagc tccccgtgtt
     2041 cgtaaagatc tctgctgaac tcacacgaat gcagggtgaa taatatggct gaaacccgtt
     2101 tccatggcgt gcgggtccgg gagaatgcgg atctggtcac ggccattaat gatattgact
     2161 ccagtgtgat tggtatcgtg gctgttgctg atgatgctga tgcaaaaaca tttccgctgg
     2221 ataagccggt gctcctgacg cgcgttatgg atgtgctggg taaggccggg aaaaccggca
     2281 cgctgtacaa aagtctgaaa gctattgccg atcaggtcag caccagagtt gttgtggtgc
     2341 gggttgctgc tgcgaaggag ggggatggtc agccgacaca aacgcagctt gttattggtg
     2401 aggtcaaaaa tgacggaacc tatacaggca tgcaggcgct cctggtggct gaacagaatg
     2461 aggctgttgg tgttcgtccc aggatcctgg ctgtgccatc gcttgacacc aaagaggtgg
     2521 tggcggcact ggtaaccgtc gcgaaaaagc tgcgtgcctt tgtgtatgcg ggctgtcccg
     2581 gctgtaatac cgtgcaggag gctgtcacct ggcgcgccga tcttgctcac cgtgaagtta
     2641 tgctgatctg gcctgatttt atcgcgtaca accctaacag tggccagaat gaggcttttc
     2701 ctgcatctgc gtatgcgtgc gggttgcgtg cgaagattga ccacgagcag ggctggcata
     2761 aatccctgtc caatgtggca gtgaataatg tcctgggtat ttctgtgccg gtgttctggt
     2821 cgctccaggc agaggacagt gacgcggaaa ccctcaatga caaggagatc accacactca
     2881 ttaaacgtaa cggcttccgt ttctggggta accggactct cgacaccact acctacacct
     2941 ttgagaccta cacgcgtacg gcgcagtcgc tggcggatac catcgccgaa tcgcagtttg
     3001 aaaccgtgga caagccgctg acgccgtcga acgtgaagga tgtgttaagc gatatccggg
     3061 caaaactgag tgcccttgtg acagcgggta aacttatcgg ggcagagtgc tggtatgacg
     3121 tggtggataa cggcacaacg gatctgcgtc aggggattgt gcggatccgc tacagatata
     3181 cgccggtacc accgcttgaa gatctggagc ttcaccagac gtttactgat gagttctttg
     3241 acggtgcatt tgcatcactg ggaggtgagt aatggcggta ccaaacaaac ttcgcgtttt
     3301 cacggtgtac atcgatggcg aaaacaagct ggggcgggtg acgtccttca cgccgcctaa
     3361 actgacccga aaaacggagt ccttccgtgg tgccgggatg cccggcagtg tttccgtcga
     3421 ctttggtctt gatgatggtg cactggatct ctcttttgcg gtaggcggcg cagatgccca
     3481 ccttttctcc cgttatgccg ggtcacttga tgaagtccgt cttcgctttt ccggtgagta
     3541 ctattcggat gcggatatca gatatgtgga tattgaggtc cgcgggcgca tcactgaaat
     3601 cgatatgggg gaggcgaagc agggtgaaga cactgagcac agttattcga tgaaaaacac
     3661 ctggtacaga ctgagcgtgg atgatcggga aaaaatcgaa tttgatgtgc tgaattttat
     3721 ctggcgcatt gacggtaagg atgtgctgcc ggatcgcacc cgctcgatgc tgggcttctg
     3781 attattgttt aacagagaac ggcggggatc cagccgtgga gactgaaaat ggataaagaa
     3841 acggaaatga ccactgacac tgccgtcagt aacggggagg cttccgcggt ggcggttgcc
     3901 gctacggctg cacctaagaa cgtggtgaag ctgacggtgc ctgttgtgcg cggagatgtg
     3961 tcgtacagtg aaattaaaat cggaccgctg gcgaatcagg ccggatcgct gcgtgggctg
     4021 tctttgcttg cgctttacaa catgaactgt gatgccatgg tcacctggct tgcgcgtgtg
     4081 acagagccaa aacttaagga ggccgaaatg tatgccatga atacggcgga ttttacggcg
     4141 ctgagctcgg cggcggttaa ttttttcgca ccctcggggt cgaatgcacc ggcaccggag
     4201 aacacggcag agccgtaacc tgtctttgct ttgactgtat tgaagatccc gttgcggata
     4261 ttgctgttat ttttaactgg ccgcccgcag agatatttgc aatgacgcct gcagaggtgg
     4321 tcgcctggcg tgagcgggcg gctgccagaa gcggaagtca caatcatgat gaataatctg
     4381 catatcaggg ttgctctggc ggcggcagac accctgacac aacctctcag gcgcgcccgc
     4441 gaaaccgtgg gcgcgctttc tgattccctc cgtgaaactc aggactccat ccgctcgctt
     4501 gaacggcaga gtgaagcctt tgctgcggca cagactaaat ttgacgaagc cacccggaaa
     4561 attgccgctg ttcagcagca actgaaccgt ctgcagcagg cggagcgtga gggtaccgtg
     4621 ctcagtgaac ggcagcgtga aatgatggct tcgctgacca cccggcttga acgtctgaac
     4681 cgggtgaggg atgtacatca gcgacgcctg gaggattcac gccgggagct ggagagacac
     4741 ggtattgccg cagagggcag cagtcagacc atacaggcag ccattcgccg gacggcagag
     4801 tataacgaag agctggcgcg acagcggcag gcgctcgcac aggtgacccg tgcacagcaa
     4861 cgctatgaac aggcgcagga gcgactcggg cggatacgta atgccggtgc ggcggcactg
     4921 gctgccggtg cagcaggact ttacgctggc gggcgtgctg ttgcgccggt gatggatctc
     4981 cagcgtcatg gtgccgtcat tgcggctcag gcaggagagg gcgcgtcaga tgccgtgggg
     5041 tataccgggg ttataaacca catggttcat accggtatca caccggatgc acaactggcg
     5101 gctgaagctg tgggtgctgt ccgctccact ctgggtgggc tgggggcggt tagtgagaat
     5161 gaactgaccc gaatcacccg aaaaatgctg gatatgcagg ccgtgatggg gggcgatatc
     5221 ccgcagcaca ttcaggcggc tgccatcatg atcaaaaacg gtcttgcacg cagcagtgac
     5281 gaggcggttg atctgatggt ggcgggcatg cagcgcatgg ggccggttat gcgcgatgag
     5341 ctgcctgaga tccttcacga gtattcgacc cattttcgta acatggggct gacagggtcg
     5401 gaaaccatga cgctccttgc ggatatggcg cagcagggga agtttgcgct ggataaaacc
     5461 ggcgatgcca tcaaggagtt cagtattcgc ggctccgata tgtcaaaggc gagtgctgaa
     5521 gcgtatgcgc ttatcgggct ggatgcagcc aggatgtcat cggctgtagc cagtggtgga
     5581 acggctgcac gggctgcgct gcagaaaaca gcctcgggtc tgttgcagat cgtcgatccg
     5641 gctgagcggg ccaatgcggc catcgcactt tttggtacac cggttgaaga tctgtctgtc
     5701 gatcaaatcc ctgcctttct tggcgcgctg gcgcgggtgt ctgaccgtat ggctgacgtg
     5761 tctggtgtgg cggaccggat ggggcaaacc ctgcgtgaca atctgagtgg tgatgtggcg
     5821 cggcttaccg ggacgttcag cgcgctgaga agtgggatta tcggggagat tacggacgat
     5881 ctgcgtgggc ttgtccggat ggccacagcg tggggtgagc gtctgcaggc gtgggtgaat
     5941 gcgcatccgg aactggtgcg aatgattgcg ctgacgacgg gagcggtgct ggcgttaacc
     6001 acagcgatgg gggcgttgtc cgttgcggcc tggatggttg tcgcaccgtt actgaaactg
     6061 cgtctggggc ttgccctgct tcgcgcgatc ccggccggac tctctgttgc cggtacgctg
     6121 tttggcggac tgacgggtgt gatttcagcc gtgggggctg cgctgggggc gctgacctgg
     6181 ccggttgtgg tggcggtggg agccctggtc ggtggtgctc tgctgatctg gcgctactgg
     6241 gatcgggtca gggcgtttgt gagcggattt attgaagggg tacagtcagc actggcgccc
     6301 ttcatggcga cagcctcccg ttttgcgccg ctgctggatc tggtttcagg ggcactgtcc
     6361 cgtattctgg gatggtttaa atccctgtta acgccagtct cctccacgcg tgatttgctt
     6421 gagcagtgca cctctgccgg gcagacgttc gggagcactc tggccggtgc gttagccacg
     6481 gttttttcgc ccctgaaagc tctgattgac ggtctggcgt gggtgctgga aaaactcggt
     6541 gttctgcccg gtgaagctga gcgggttaag cgaaaactgg atgaggcccg taaaacgccg
     6601 gtggcatggg agtgggatcc ggaacagaaa aaaatggttc agaaggcgtg gcactggtcg
     6661 cctcctggtg gggccagtgt gccttccggc gcctccggta cacagcctcc gccggtggcg
     6721 ctccctgcgg ggatagcgcc ggacggtaac cgtaacgttc tccgtcacct gaaaggcatt
     6781 gaggggaata ccggtggtct gctggctgaa aaccgtaaac gtatcggacc cggagatatt
     6841 gtgtttaaac atcttccacg ggcgctggcg gtccgggggc agtggcatga acctgtcgtt
     6901 gcgccgtctt ccggcggaat gcgcgcgctg ccacaggagg agattcaggt gcagcaatgg
     6961 ccgacggcac gcccggcgcc acgaatgcac cagcaggaaa ccttcgccag cgcctttcag
     7021 ggggagatac atgtgcatct tcacggggtg agttcgcata atccccggga actggcgcgc
     7081 atggttggtg acgctgtcag ggcggaaatg aacagaatgg cacgtgccgg aaccggcagc
     7141 ttccgcgatc gcgactgagg tgaaaaagat gatgatgatt tatgggatgt ttgtgtttca
     7201 gttaagtacc ctgccgcatc agcaggtgga acagagtaaa cgctggcgac acgtcagtaa
     7261 tgaacgggtt aaccgttcat cctcctggca gtatattggc tctggtgaag atcacatcac
     7321 gatgtccggt gtgctctgtc cggaaatcac cggcggggag gtgtcactga ctgccctgaa
     7381 caccctggcc tacacaggat taccctggcc actgattgat ggtgtcggac agatttacgg
     7441 gatgtatgtc attacggaaa tcaggaccac ccgtcaggag ctggatcgct atggcatggc
     7501 ccgcaaaatt gagttcactg tcagctttca gcgatgcaat gaagatctgc gtgagcgtct
     7561 tcagtcgggc tcggtgggtg atctgatggc aggattaaaa aacagcgcgt cgtcggccct
     7621 gtcgtcggcg tcctctgcgc tgtctggtct catgttgtga aggtcactgt tcaggcttcg
     7681 gcttcttttc cccagccaac cttatagtcc agaatcgccg ccgtatcgtt cagggcggcg
     7741 atctcttttt tcatgttacg ctggcgaatg tgaatttcca tccctttggc aaacagggct
     7801 tttgcggctg catcgctgag ggcaatcagc tcatcagcag aaacaggaac atcgttgttc
     7861 tgtgcatcag tccagaaaaa atccagcggc aggatccccg cttttgcggc agcaacggaa
     7921 ggctcaaggc gggcctgcgt tgacttcccg tagtcccatt tcctgccgtt atgttcaaag
     7981 gtgtaactcg ccgcttccat cgcattacgc caggcgttga tttcgtcgtt tttcaggcgt
     8041 ctggcgtttt ccctgtcagt gtcgttttcc tggtattcag atacagggcc gtgatttcct
     8101 gctttcagtt cgccccagag ttttttgccg tgtggcgagt tgtcatattc ggttgccgta
     8161 tagggcagcc agctctcctc cccttcaaac tggatctcac agtcgatagt gccgtcacgg
     8221 ttataagcgg catttcttgc tgatttgatt gtgtgcatga gtctctccgt caggcaatgc
     8281 gctcaaatat tccgattcct gcgcctgcat aaatgtcagc gcaggcccgc cacgtgcccg
     8341 gtggcctgcg gtttgtgttc cagttttgca tggattcacg ggggccgaag ttgccttcgg
     8401 aatgagtcag gcttatcagt ctgagtttac tgcccgggcg ggtgtttccg gccgttatat
     8461 caattttggc tggccactcc ccttcggcat ccgttgccgc cagaataaag ctgcccacat
     8521 cccagagtgt ccgctgtgtt tttgcctgtg cagcctccag tgcttgccgt gctttctgcc
     8581 cggcgttttc tgctgccgtc atggccgtct tcacagcttt tggtgtggca gccagcactt
     8641 cactgtcact gtccgttgcg ctgcttagct gaacaatacc tcgctctgtc agggaggcgt
     8701 cgccggtctg cgtgaggggg agtatggcaa cctccttcca ttcagaccat ggcccgttgc
     8761 cggtgaagtt acctgtcact gtccggataa attttcgtgg tggattccag gcggtgtatt
     8821 cctgctggca gaatccccat gcgcccatcc tgatggataa tgatccggct gatgttaccg
     8881 ggtagtgatg tgctgtagtg gcgccgttgt tgttgggctg atagtagtca ccatgatgtt
     8941 caggtctccc cagtgtattc agatccacgg cgaggggggc tgttggtctt gttactgtca
     9001 gcgccttaag tgcttttatt gctttgggtg tggctgcccg ggtttcatca tcactgtcgg
     9061 tttcactact gagctgagta aatcctttac tggtggttgt ggcatccggg tgtttaccgt
     9121 tttgttcatg ctgctttatc atttctgcca cgccagcctg tgttgccatg gtggttgatg
     9181 agtcggctgt cagatttaca gaggcttcgt tgctcagcag aatcaccatg cggaatacct
     9241 gttctctgcc tgagccctca tcaagctgcg gtttgtaacc ttccgcaaca ttacccacgg
     9301 cgatgagtgt gcctttgctg tcaaacagac cgatttcgcg gatccagaat cctccttcat
     9361 tcgcaggaat gatcacttcg gcggttaaaa aactggtgtt ttccgcgtca cggctgaccc
     9421 tgctgacctc gccccgccag acctctttaa cgagagcggt ttgcgtggcc agtggttctg
     9481 tgggtttacc gttaccgtct ccgacggcga tatgtgtcag cgtgatcttt tctccatccg
     9541 gtaacaccgc agctgcgagg gctgctgtgc cggcatttgt gataatggtt ttgtattttg
     9601 cggccatcat tactccttgt ccggtctgat ggttactgtg atgccttcat aggtgactcc
     9661 gcccgggtgg atgtcaccac tgactgacag cgggcttgtc agttctgctg ccggatatac
     9721 cacgatggtt tccccggtca gcgtggcccc ggttatgcgg gatacacccc gtgcaacgta
     9781 ggtcattttc atggcggcaa ggtgtcggct taccgggcga acggtggtaa tcattcgctc
     9841 catctcctgc cgggtttcct ctgtcagtcc gcgctccgat atgtcaatat ccagcctgaa
     9901 tgaccccggc ggatcgttgt tctcccacca ttctgtaata ctcagtatcg cgccgaaggg
     9961 ctccacagcc cgacgcatgg cgctgatggt gcctttgtgg cggtgaatat gccacgagtc
    10021 acggatgatc tgacgacgtg cctcttcact ccatgtttca tcccagcggt caacggaaag
    10081 cgcccaggca agccatggca gcagatgggc tgggcaggtg tcggggttcc agagggtgtt
    10141 gatatggatc ggcagctctg acaggcgctg agcgccctgt tccgaatgat gcaggaagga
    10201 gggcgatgat ggcggcagca gtcggttatt catcggtgcc acctgcagag atgttccggg
    10261 atgtgcagcg ggctgactgg gtgttactga tcaccacatt ctgcgctggt ttcttcagct
    10321 cgactcgctg aacgcccggt acgtgtagcg ttgccataat ggcagagcgg gccacatcac
    10381 gccctatggc attctgagtc ttcagccatg tgtcaagtgc atctctggcg gcggcaagaa
    10441 tgggttcaga ttccgggcca ggataaaaat agagtacggc ctcaatatcg taactgacga
    10501 tggtcgcact ctgtaccgtg agtctgtcgg ccagggggcg cacagtttca tcattgagag
    10561 cccggctgac ggtcgcgatt aattccggtg acgctgtgcc gtctccttcc gttgagagaa
    10621 tggagacaat gaccgtcgca ggtgacggac tggttgcccg tgcatcggca acaagaccgc
    10681 tggcactgcg ggcaaaatat tcatatgcag cagaggggcc tgcgacgctg aaaccttcaa
    10741 atccggattg tgcccgcaga cggaacgcgc tgtcgctctc catgatggca gcttctgtat
    10801 cagtagcagg tttcagttca aggcgtttcg cattcacgat agcggcgaga ttatccaggt
    10861 ctgtggatac ggcatggctg agcatgcacc ccgccgctgc atcgttgatc cgctgacgga
    10921 gcagcatttc gcgaaaggcg atggcctgag caatgatgtt gagtggctct gattccagcg
    10981 caatggctgc gctgacggca ctgcgcatgt tttcagggaa ggcatcaatc ataaaggcct
    11041 tcacgtcttt cagtatgctc tcataatcca gtgattcaat gatctgcgga cgtggtaact
    11101 gagacaggtc aattgtcggc attactcccc ctcagcggaa tgctcatctg tacgggctgc
    11161 attgtgcggg tgagcacgcc tgagatggcc agcagagcgc gtccgtcagg cagccaggcg
    11221 atatccaccc ggttaatggc gatcctgggc tcccactgtg cgagcgccat aaccaccgcg
    11281 ctcatgcact gcagacgcgt cacgtcgttt tgtggcgcat cgagcagttc aggtaacagg
    11341 ctgccatact ctcttcgcat gatgcgactg ccgcgcgggg tggtcagaat attgtttaca
    11401 gagtggcgga ggtgttctgt gtcactgatg catccgctac catgcgggtt catgcctgtg
    11461 cataccggtg tcatttcggc ccccctgtca tgcttccgcc ggactgaaca ccgttatgaa
    11521 cgtgcgtatg cacggtaaca ccatttgagc gtaagtttcc gttactgtgt gtgatatccc
    11581 ctttcatggt gccaccgtca gtcagttcaa acgtcctggc cttcagatgt tttgtgcact
    11641 ctacggccgg tgtgtccagg gtaatacgtg tttccgcaga gatctctgcg gttttgattc
    11701 ctttcaccgt cagcgcgcct ttttctgcat catagcgtat ctcggcaccg tcaggtgcaa
    11761 ggagtatcac ttcattctgt tttgtgccgg gcgccgggtg ttcttcgctg tagagactgc
    11821 cagccacaac ggccatttca ggactgccgc ccgggcagag caacagcacc tgttcaccgg
    11881 gggagggggg cagccagatt ttaaacgccc cggcgcgtgt gcagttccag cggagccagt
    11941 ccgttgtcag tccgccgctg gtaatgcgaa tgcgccagtt tttcagatcc acctccgaaa
    12001 cgacgccggt gcggaccatg ttttccagca gtcgcatgat ttcagcattc aacgggtgtt
    12061 tctccacagg ttgttgataa tgatttccgt cactttctgt ctgtcggctt cactgaatcc
    12121 cagcagttcg cgttgcggat agcggacggc attaccggca gagaccagtg actcttcgcc
    12181 gaactgatga acgcgggcaa tgcgggctgc gcggccttca aatcccacgc tgacgccgcc
    12241 gggatcggtt tttaccttca gaaagcggtt tgtccgaagc tgctcaaaca tgggtggacg
    12301 cttaatggcg ctctgcgttc gcctgcgggt gtcaatttcc aggtattctt caatatcggc
    12361 tttgtggaac gagcgtattc cccggcgctc cgtgtcgtaa ccggtgatca tggggccgta
    12421 ccggccggtt gttgtccgcc agtttttcag tcgtctcacc tgagtgccgt tccagatgaa
    12481 ccccacacct gcctggacac ggaatacacg ttttttacgg gctggccacc ggttaccgtc
    12541 cggtgattgt tgcgaacgta tacgcttttg ttgcgcggcg cggacagcct gaccgacagc
    12601 acgtgctgtt ttgtttatac cggcagcgga cagaccactg aggatctcct gaaagagacc
    12661 gtccagtctg tgcaggtcat ccggagccat cattctgacc ccatgtgaca tcttcgaggg
    12721 tgacgcgcca tgcttccagg gcccggctga caggcggctc tccgatgtgt tctgcgtgaa
    12781 cagtgccatc atcattacgg cggataatca cccgctccca taccgggatt tccagcagga
    12841 tatcggtgct gtcgtcgtca ttgatggcaa cagaaaattt gatatccctg tttttctccg
    12901 gattaagcag gagatcaggc tgattgcggt acagccagcg catcagcggc accatcagtt
    12961 catcgagatc ctgcgtgaag tccatgacaa agatcccaag cgtgtaacgg taaagatatg
    13021 agggggattc gccggtcgtc tcaatgcctc cgttattcac atacacagtg aatttctccg
    13081 ggttggcgcg acaccatggg caggcgtcag tcatggcgtc gcgcagggtg ttaattttca
    13141 gcatgccccc tcctgtgtgt tatggcatgt tctggttgtt ctgttgtgca gacttctgac
    13201 tgattttttc agcggcgcgc tcgtcaattt ttcgctgaaa ataatttctg ataacactgt
    13261 aaccgccgct gatcagatac agcgcgcaga ctgcagtaca gaaatacagc atgatctgat
    13321 gaatgaataa cataatcacc tcacacaaat gattgacata acgatgtctg gcgttttaaa
    13381 aaaaatgaat aacaataata aacactctcc ataattcctc ttgcgctccc gtcaggtatc
    13441 ttttcctctc agtcttgcct gccgggagcc tcttttttct tatgcggcgt tgctgttatt
    13501 gatatccacc gtcacatact caatcagcac ggggtaggtg tccttgttac tggtgatatc
    13561 cgtgatcagc agtttgtcgt tatccagtcc tgccgccggg catttcgcca gattaaaggc
    13621 cgcctgggtc tgaccgttga tgaccggtgt cacgctgatg ctgttatttc cggcaaaccg
    13681 gaacaccacg ttgtgccagt tatgattaaa tgccccgaag gttcccagtt ttgcattctg
    13741 ttgtttgtgc tgcatcagat tcaggtttgc gctgtcgctc tggatgaaga aggctgccag
    13801 catattgttt cctgtgcttc cctccggaac aacgttttca ggtagctgag aaaccggcca
    13861 gtacagagcc atggcgaagc ggttttctgc cagctgaaca ccttccggga ttttgaaacg
    13921 aaaggcaatt tccccgcctt tctgaagtaa ttcagttgct ttacttgcag gcacatcgtg
    13981 ggcgattttc caggctgttt gttgcccctg gactttttcc actcgcacgg ctttaccgct
    14041 ctgagcagca gagtcattta cgacggaggc ttttccatct gcccctgtcc agccctgttc
    14101 tttcagtttc ccttcctgtg cgtctgcgcg ataagcgacg gccgttgttc ttgccggtga
    14161 tgactgcgta cctgcgcccg gctgaggtgc aggtgtggag ggctgcgggc tttccgtggt
    14221 ggtgctgtct gaaccggccg tggctggagg tgctgcgacc acgctgaacg gtgcgggtgc
    14281 acctttcagg aaggcgacgg tcttcccggc atgcatcagg atcgccgttg ccaggcggtc
    14341 agaaataatg ccgcggcggg cccatgaact gaaatgcgtg gcgcgatctg aagaggtcca
    14401 gtttccgtta ctacgggatg cagcaccgta ataaccggct tctgccaggt caggatcttc
    14461 ggcaggcagg tttgttggcg tgttagcacc tttctcatcg gtcatcaggg gcacaaaata
    14521 aattttttgc gcttctttgt tctgatatgc accgtaaacc accgggtact gagaggtata
    14581 ttgttgtttc cagatatagg tggtatcgcc gcaaatccag ggaacaagtg atgcgttgcc
    14641 tccggtgcac tgtgcagcat atgcgctgag atcggcgcgg aactgattca ccatgttcag
    14701 gaataattgc gggtgctgtg cgtatgttgc tgatttcaga tcaaactctc ccagcatcca
    14761 gaccacggat aacaggacgt ttttggggtt tttatccagc gcggcttttg tacgggtaag
    14821 cagatcgtta tacagcggtt tgccgacgcc ccacagggat gattcggccg ttgctcctga
    14881 agattcactg aatgccccct cagcgccgga agtaagcgct gaccctccac gacagcaagg
    14941 taccaggagg atccctgcat ttttcgggat aaacgccagt aattttttgg caatatgtaa
    15001 accctgtccg acgcagccat actgaccttt tgacagatcg gcgttcggat gattaaggct
    15061 gctcatatcc tgtacatcat ggagacagtg atctgccgga ataatatcgt tataggtaca
    15121 tgcagcgccg cccggtgtta ccgtactgcg acgcgctaac tgtttaattc gtggatcagg
    15181 actgtcgaat gtatcgggta atggcagccc ctcaccgtat gccatgccat tggactgacc
    15241 agccagtggc accacataat aaaactctgg ctcagttgtg tgtccagtgt cgtatccgtc
    15301 ggtagtgccc ggtacggggt tttcctcgcc ttttggtacc ggaatggctg atggcaggtc
    15361 tctgtcagtg accagtgcct gcatcaggta aaatggtgct cccggctctg atgctgagac
    15421 aggtccccca agaagtcccc atccttccct gagcatgggc gcaagttttt cggcaagttc
    15481 cttgttcgtc attgccgtca caagctgata atgtttcggt accatagtga ctccgttatt
    15541 tttcatgagt gttcattcgt gctgtccgct gccgctccag cccggcaatg gcggctttat
    15601 cggcattaca actgtccagg gcatctacca gtcggtctcc ccagtcagcc acatcagccc
    15661 atatcatgtg ttgcggcagc accggtaccg gtgtctgtct ggtcagactt tccggcacgc
    15721 tcccgcaaat gacgccggtt ttcagcggcg ctggcgtgtt gttgcaggct gtcagcaaca
    15781 tcagcagggg gagaagtgcg tgcgcactca tccgcctgta agtttttccg catcgtttca
    15841 cgtcgttctt cccctctggc tgcacgctcc tgatcttcgc gccggatagc ggccagcgct
    15901 gactgtgtct ccgccgtggc cttaagcaac tcccgtacgg tcgtattgct ctctttcacg
    15961 gctttttcca gcgcttcata acgggtgctc tcctgacctg agcgataggc ccggatgacc
    16021 accattgcct gcagcgaaat agtcagcagc agcgcacaaa ggataatgtt cagtttcatg
    16081 gctgcgctcc cgcgtctttc aggcaccagt tcttaaaatc attccggcgg ttaaccagtc
    16141 cctgcgtgta cgttccgcca ctgttcacaa aatctgtcag ccggtaacac atgaggcgcc
    16201 attgccgtgt ctgcgcgtgt ttccagatgg tggtgcgttg cggcgtgccg tttttgtttt
    16261 taaaccacat cagattccgg cagcccaggt taaaggcggc gtccgtcatg gcttcgaatg
    16321 cagactgcgg catctgctgc cccctgaaat tctgtgtgat gcagttctca gcgcggcgca
    16381 tatcgttgat ccaccgcccg gccacttcct cctgtgtgta caggcggttt tccacgttac
    16441 cggtggatcc gcatccgacc gtaccgaccc ccaccagatc acggtatggt cgggcccggc
    16501 actcctccca tgtggcaatt ttgatttgtg cctcgcggga ggttctcagt tctccgggag
    16561 ccagggagat ccccagcgcc acaatggcgg ccacggcgta acgcttaggt ccggccatcg
    16621 tgctcatcct gcttcagaag catcattgcc cggcgctctg cgtcgttcag ctcccttcct
    16681 tctgactgca taaggatttt ttcaatcagt tcgttacgcc ttgcctgggc gatctccatc
    16741 ctgcggcggt gaagccaggc acgaagcagt gacagcgtac ccagcacaaa accggccacg
    16801 gcaatttttt cgctcagcgt catcacgccg atcccggctg tcgtcactga cgcccagaac
    16861 gtgatgtgat cacaaagccg ctgaaacatt ctcaccccca caactggatc atttcacgcc
    16921 gggtctgccc ctgcagctcc ggcagctcca cttcctgtcc ggcatgcaga aacatgacgg
    16981 ctgaaatccc ctgattggcg gccagtaccg cctcagtcac cccctgcgtg gtgccgtaat
    17041 ggcgctgaca gagtaaatcg acggtgtcac cgtccagagc cattactttc atcagaccag
    17101 ctccgcataa atgcgggtga caccgcgtat gtctgatatt gcccagcggg catcccgcca
    17161 caaatcctca acctgcatgt ccagcgcgtc agccctgcgg tcgcctttat cggtggtatc
    17221 cacatcccgg gtgcgctcca gtacaaacgc gcgggtgagt gcgaaaacgg ccctgcggta
    17281 gcggtggacg tgtacactct ctccgttcac ctgcgtggcc ggaacgtcag ccagtgatgc
    17341 cgcgccttca gccatccgct cacgtttcca gagctccagt tcggcgatca catgggcgac
    17401 cgcttccgtt gctgcatgta acagacgggt tgttgttgtc ctccccggaa tgcgggaagc
    17461 caggcgaatt tcatgcagtg agatcgccgg ccagaagtca cccgcgctca ccatggcgtc
    17521 gccgtcatcg gtgtcggtgg tatctgtgtc agcgctgttc acgcggggtc tggctatcag
    17581 actcatgcct gtctccgtaa aaatcaggcg gtgggcaacc ggtaaaaaga acgcgaagcc
    17641 tgttcagatc accggttgcg ccgcctgccg acgaggtgcg gttcgttaag tcagtttgtt
    17701 ttgcgcgcgg cttttttctg gctggcagcg cttttctttt ttgcgctgct ctttttcccg
    17761 ccggtggtgg ttttcctgcc cgttgtggat gcaggcacca cctgatttcc gtctgccagt
    17821 ttcctgaccg cgcgctccag cgtggcaatt tcacgcttca ctcccgaagc cggattgatc
    17881 cgcatggctt cacgtaacag gctcagcgac agacgcagat catcgggaga ggaggaggcg
    17941 cggcgggaaa atgcgcgtgt cttgtgcagt tttgacctga cctggtccgg catatcctgc
    18001 tcactgacca ggtggttcag ctcatcaagc atggcggtgt acacagacaa atccgcctcg
    18061 ctgtccgttg tggcctgcgt cagaacggcg tcagtaatct cttcagccag taccgtggcg
    18121 gcgtcacggc caaagttatc cggcagcgcc agaccatggc ggatcacgta ctccccgata
    18181 cgcagtgcca gcgggtagtc acggcagtca atggcccaca ccatcatggt ggtgatgacc
    18241 tcatcctggc gtccgctgtc tcctgccagg gtgccttcaa tccacccttc aaagtcaggc
    18301 aacagatgtt ttttaagctc tgccctggcg acgtttgact gaataccctt cagctgcatc
    18361 tgtgccagcc ttaactggtg aaggacctgt tcatgtgcgg ttctttcccg ttgtgtttcc
    18421 cgggcgttca tggtcacccg gttgcgggcc accgcctgtt gcacatagcg ttgtgccggt
    18481 gtcagcattt ctcagctcct gttaacgggc gcggcgcgcc cgtccgggtt atgcttcggg
    18541 ttgcggctgt ccgctttcgg cgttgttgtc cggatcttca cctgcgaagg tgatcccctc
    18601 aatcagcgcg cattttccgt agtcttccac cacgtagcag tcattcatgg actggtaagt
    18661 tgcgatacgg ttgtattccg gctcctcgcg aatgaaacgg cgcagtgagc ctttctggaa
    18721 gtagatcgac aggtttttca gcgacgtgat gagcatggcg tctttcggga agtacggcgc
    18781 aagatacgtc tgaagaccac cgacggtacg ggacgccaca atcagctgcc cggcaacaag
    18841 ctcagtgttc gggttggtgg tgctgatggc gttaatcatg ggaagacgca gtgtattgaa
    18901 cagatcccgc cccatcagta cgaccagatc cggtgcgtcc ttatgccatt catccagcac
    18961 ggatgagcgg atgtcgtgca ccatggcatc cgggttacca tagtcacctt ttgccaccag
    19021 tttgttttca tcatcgcgac gggtcagggt catccctttc accacgcggg tggcagcctt
    19081 gtcgcggatg tgtttcagcc agcccgggtt gacgtcctgc aggagagggt tagtggcacg
    19141 atcggaaatc agcgcgtgtg actccccgtt gaaaccaatc atgattcgat ccagggctat
    19201 ctggcgggtg atttgcgcgc tcagcagttg ctgaaagttt ttctggctgc tccatgcgtc
    19261 aatctgtgcg tagctgatga aggtatcgta gttcacctgc tcgcagcggt aacggcgtgc
    19321 agccagatca gcaactgatg cagggttgcg gcgtttcact ccgtcgctcg ttgcgttcgt
    19381 gctggcaatg gtgccgctgg tgtcaatcag gactttttca ccttcctggt catccacacc
    19441 gataaaattg atttcctgta acagtccggc gctttccttg atggcgttct ccatcttctg
    19501 ctgaatggat ggatccacgc tgaaggtttt acccaggcgc tctgttgtga taccagccag
    19561 cgatgcctgc tgaatcatat agcgttccag actgtcacgt ccttcacggg aaagggataa
    19621 acgggccata aattactctt ctcctgtcag aattcagcca gtgtctggtc agtgccgcca
    19681 gcggaagtaa aacgctgctg tggatcggca tcctggtttc tcagttgcgc tttcagtgct
    19741 gcaagctcgt gactgagctc atccagcgcc gttttgtcga gggcatgttt ctgctccaga
    19801 gtgctgaaac ggtcgataag atctgcatga tcggatgcga tgttttccac aacgtcgcgc
    19861 agctggctga actgctcgcc atccgctgtg cgtgttttgc tgatgagtgc catgacgcgg
    19921 ctgaaccact gacggctctc ttccgttcgc tgttctgtgg tggtaagcat ctctgcttcc
    19981 atggcgacgg tgaacatggg gggttctgtt gtgcacttac tgaactgcat gatgctggcg
    20041 cgttgctgtg cggcaaactc caggcgttca gtgcccagac ttgctggcgt gtcggtcatt
    20101 gccagaccca caatgtaagg ccccttgacg gcgtccagct caggcaacat ctcaatgctc
    20161 gaatacactt ttttcccgtc agcaatcatg tttttcatgc ggtcggtggg ttcaatttcg
    20221 gcatataacg ctgtacgccc tgccagtttc ccttcggtga tttcatcagt tgacagagcg
    20281 atcagatcac ccactgcgca gagttcactg gatggccaca tcgaaaggac gtgctcaatg
    20341 ttcacccggg aggcgtaaac ggacgggtca tagttttctg cggcctgacg cagcatttca
    20401 ccagaaattt cacgtccgtc gatggttgaa ccggaaacgg caacgcgaaa ttttttgcgc
    20461 tctggcgtgt ttttgcttcc catggaaatt ctccataacc tggtcgtttt tcccatgatg
    20521 gcggcagata acggtgtgtc tcaaggtgct taagttgtat ggcgacgggg acaacgcggg
    20581 ctggtatcag gcggtattgc tgaggcttaa tcttgccctg ttaatgtcag ggggtgcaat
    20641 gatccaggat gtctttattc gtatgcgggc gaaacagctt tactggcagg ggtatacccc
    20701 ggcggatatc gctcagctga tgggaataaa ccagaacacg atttattcgt ggaaaaaacg
    20761 cgatgagtgg gatgccactc ccccgatcca gcgggtcacc cagtccattg atgcacgcct
    20821 gatacagctc accgccaaac cggataaaag cggcggtgac tttaaggaaa ttgacctgct
//
LOCUS       DAZFQD010000087        20844 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.087, whole genome shotgun sequence.
ACCESSION   DAZFQD010000087 DAZFQD010000000
VERSION     DAZFQD010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20844
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.087"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..242)
                     /locus_tag="ACHKTW_003228"
     CDS             complement(<1..242)
                     /locus_tag="ACHKTW_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO6464197.1"
                     /translation="MIQDAFVRIRARQLYWQGYTPAEISQLMGISQNTIYSWKKRDEW
                     DATPPIQRVTQSIDARLIQLTTKTEKSGSDFKEIDLL"
     gene            400..1236
                     /locus_tag="ACHKTW_003229"
     CDS             400..1236
                     /locus_tag="ACHKTW_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GPO family capsid scaffolding protein"
                     /protein_id="HFO6464198.1"
                     /translation="MASKNAPVRKKFRVAVSGTTIDGREISGQMLRQAAENYDPEVWG
                     SRVNVEHMLSRMPSSEFSAVGDVISLSTEEIREGKLAGRTALYAEIEPTDRMTQMLND
                     GKKIYSSIELEPNIDAVGGPYVIGLAMTDTPASLGTERLKFAAQQRASIMQFNSRSGE
                     PVMFTECMEAELAASVQDSTEESQKWFSRVMALISKTRDTDSEQFAHVREAVESIATE
                     QAGLLDRFHAMEEQQRSDKASMEALTRELTELKAQLKTEDADKEQRFASTGGNSHALA
                     DF"
     gene            1252..2346
                     /locus_tag="ACHKTW_003230"
     CDS             1252..2346
                     /locus_tag="ACHKTW_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019028 - viral capsid [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein, P2 family"
                     /protein_id="HFO6464199.1"
                     /translation="MGNRLTLSREGRSNLEAYLARQAELAETTVERLGKTFSVDPAVH
                     QKMENAIKESDELLKRINSIGVDDQEGEKVLVNTSGPIASTNSTSDGVKRRNPADVSD
                     LASRRYRCEQVNYDTFISYAQIDAWSNQKNFQQLLSAQITRQIALDRIMIGFNGESHA
                     IISDRSANPKLQDVNTGWLKHIREHATTRVVKAMTLTRRDHENKLVAKGDYGNADAMV
                     NDIRSSVLDEWHKDAPDLVVVMGRNLFNTLRLPMINAISTTNPNTELVAGQLITASRT
                     IGALPTFFAPYFPDDAMLITSFSNLSVYFQKGSLRRFIREEPEYNRIATYQSMNDCYV
                     VEDYGKCALIEGISFAGEDPEDKKEAEAQA"
     gene            2396..3205
                     /gene="gpM"
                     /locus_tag="ACHKTW_003231"
     CDS             2396..3205
                     /gene="gpM"
                     /locus_tag="ACHKTW_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit"
                     /protein_id="HFO6464200.1"
                     /translation="MLTPAQRYLQRAMAHHRADPFNREEQRTRTAHEQVLHQLRLAQL
                     QLNGVQSNIAKAELKKHLIPNFEGWIDGTLAGDSGRQDEVITTMMVWAIDCRDYPLAL
                     RIGEYVIRHRLALPDNFGRDSATVLTEEIAEVVLTEAATNSETDLSGYIRMLDELDAL
                     VHDQDMPDQVRAKLHKARAFSRRASGSTEDMTVSLGLLREAMRINPAAGVKREIATLA
                     RKVSKVQPEPEKPAEEKSKSKATSGRKKPTTKATARKATKKTAAKPARKAT"
     gene            3314..3808
                     /locus_tag="ACHKTW_003232"
     CDS             3314..3808
                     /locus_tag="ACHKTW_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head completion/stabilization protein"
                     /protein_id="HFO6464201.1"
                     /translation="MSMVARPRVNDATDDTTDTDDGEARISTDAFWPDIVLKELRLAM
                     RIPGRTTTSRLLHSATEAVAHVTAELDAWKQKQEAAGRSTLADVPAAHINGESVNVHR
                     YRRAVYALTRAGVLERSRDVDTTDKGDRRADALDMQIEDMWRDARWAISDIRGVRRIY
                     AELI"
     gene            3808..4008
                     /locus_tag="ACHKTW_003233"
     CDS             3808..4008
                     /locus_tag="ACHKTW_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail protein X"
                     /protein_id="HFO6464202.1"
                     /translation="MKVTAHDGDTVDLLCQRHYGMTKGVTEHVLAANPGLSNTVFLTA
                     GQEVELPEITQEKQRETVQLWD"
     gene            4011..4301
                     /locus_tag="ACHKTW_003234"
     CDS             4011..4301
                     /locus_tag="ACHKTW_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO6464203.1"
                     /translation="MFQRIYDHITFFTSVMVTGIGVMTLSEKMALAGLILGALSLLRG
                     WMHRTRMEQAQERRNELIARIADQMQHRPLKGSEKRALEALNADGESHARTD"
     gene            4285..4824
                     /locus_tag="ACHKTW_003235"
     CDS             4285..4824
                     /locus_tag="ACHKTW_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO6464204.1"
                     /translation="MPGQIKRYAVAAIVALGFSLAPGELRTSQEAQEKIATWEECRAF
                     PYRDIAGVKTVGCGSTGNVPDRHHTKQEVAGRWVNDLRRAENCINQNFNGRNMPQRHF
                     EAMTDAAFNLGCKGLMWFKGRDGRMHRTTIWKFAQGKNWSAMCGRLTDFVNSGGRYSQ
                     GLFNRRNDFRNWCMNGVKQ"
     gene            4821..5255
                     /locus_tag="ACHKTW_003236"
     CDS             4821..5255
                     /locus_tag="ACHKTW_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6464205.1"
                     /translation="MKTPAVLLWFLATLLLCGLTGWEAYRKGKESLGREQMMDIIEDN
                     RAAMQEVRHAYTDFSRVINVIRILDQQRITEGEQRREEMQKAVQGDTCASAYPPAAVT
                     ERLQQHAAHVENQTRLRAGADSSDKADPGSGTGNTRDMVQGD"
     gene            5374..7344
                     /locus_tag="ACHKTW_003237"
     CDS             5374..7344
                     /locus_tag="ACHKTW_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFO6464206.1"
                     /translation="MSKKHFELITATNRAEFAEKVNKRLQEGWELLGNAGIASEPGVP
                     WYLMQAMVSGSDDQGGTIITPTSDSEPEWYYVVVSAGQSNSMAYGEGLPLPESYDKPD
                     SRIRQLARRSTVTPSGKACAYNDIILADHCLHDVQDMSQYNHPKADLNKGQYGCVSQG
                     LHIAKRLLPFIPANAGILLVPCSRGGSGFTTGDAGQFSEIGGATEKSCRWGTNTPLYK
                     DLISRTKAALAKNPKNVLLAVCWTQGEADLEKEQNAAQHKDLFTAMVKQFRADLADVA
                     KQCNQGNPESVPWICGDTTYHWKKTYEAQYATVYGGYKNSTEKNIVFVPFVTDEYGKN
                     TPTNEPTEDPDIAEAGYYGAASRSSGNWVSHLRGTHFSSWARRGIIADRMASAILLNV
                     GRTLPFIAGTEPERVAKPSQDAPGGISKPSSPEAEQPAVRPGPVTLMSLLASSPELES
                     QGWTLSEGKNGANTPKQLKDMLIGDEEATGKKALKLTKQEGNTWYLEHKAGNGADLLK
                     NGGTVSCRFKIEGELKKDQYAMAMYWKTSALPQDVKLLGADPNNLLLSFFLQTDSANL
                     NLMYHKTPNEQLGTFGQFNNNWHTIAFRFAGNNSTQVTPVLDGVDGKPFMLSTSKADV
                     TNDTLRVTDITYRPTYTVLIDSLSVVVNQPPA"
     gene            7409..7573
                     /locus_tag="ACHKTW_003238"
     CDS             7409..7573
                     /locus_tag="ACHKTW_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HFO6464207.1"
                     /translation="MGFIHQIMLYFSTAVSLLYLISGGYKAVRNFWNKKIDEAAARKS
                     AGGAVNGVLP"
     gene            7746..8216
                     /locus_tag="ACHKTW_003239"
     CDS             7746..8216
                     /locus_tag="ACHKTW_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO6464208.1"
                     /translation="MLKLNSLREALTSNCRWCKASPEKFTVFIESGGIETTGESPSFL
                     YRYNLVLFVMDFTESIDNIMLPVMAWLYRNQPDLLLNPEKNKSIKFSTAINDDDSADI
                     LLEIPVWERVIVRRNDDGTVTAEHIGEPPISRALEAWRVVFEDMTWRTGTETST"
     gene            8213..8848
                     /locus_tag="ACHKTW_003240"
     CDS             8213..8848
                     /locus_tag="ACHKTW_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HFO6464209.1"
                     /translation="MRADRELDVIFQDILSGLSAAGIQRTAMSVGKVVRQLQTKRIRS
                     QVSPEGQPWEKRKRRIMRIQKGVKFIWKDGQVRNLKNWSTSQGCFGQKITGYDTDAGG
                     IRTFYKADIGDYLEINTQRVAARASRRSPMFEKLRTARFLRIIPDTNGVSVGFQGRAA
                     RIARVHQFGEVGLVSPGNATKYPVRELLGLNEKDKQQITETVIAGLGKAAK"
     gene            8845..9426
                     /locus_tag="ACHKTW_003241"
     CDS             8845..9426
                     /locus_tag="ACHKTW_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001271907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HFO6464210.1"
                     /translation="MNIELMRLLENIVRIGVVTEINTDTWEVRVQTGGLTTNWIRWNS
                     GRAGKFKIWIPPSPDEQVFLLCPGGNPETAVSAGSLYSNDNPPPGSLENEMVITAPDG
                     AEFRYNAQESSLTARGIKTATITAETSITLDAPKVECTQLLKTKTFELTSGGTMKGNV
                     KHSGGSLESNGITVHTHVHSGVKSGSDTSGGPQ"
     gene            9423..9791
                     /locus_tag="ACHKTW_003242"
     CDS             9423..9791
                     /locus_tag="ACHKTW_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HFO6464211.1"
                     /translation="MSTGYTGMNHAGYGTLTDIDHLNQSITDIILTPEGSRVMRREYG
                     SNLPALLDSPANEITRLRCMSAAVIAITRWEPRVAINSVDLTWRNNGSAVLSMSYLIV
                     STMQSASISVDIQGGKDADN"
     gene            9778..10674
                     /locus_tag="ACHKTW_003243"
     CDS             9778..10674
                     /locus_tag="ACHKTW_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HFO6464212.1"
                     /translation="MPTIDLSQLPQPAIIESLDYEVILAQTKTFMISVFPEAMRDEVA
                     AALELESEPLNIIAQAMAWRELLLRQRINDGAAACMLSHSSGSDLDNLGVNMNVQRLV
                     ITEATETTPAVMESDAAFRMRIQSAFEGMSVAGPSGSYEYFARSASGKVADARATSPA
                     PAEVVVALLSADGDGTANEALINEVKAVLNDETVRPVADRLTVKSAEIISYKIDATLY
                     FYPGPESEPILQAARAQLEAWLAEQRRIGRDVARSAIMAALHVPGVQRVELTQPAKDL
                     VISETQAARCSATEIRAGGTDE"
     gene            10667..11386
                     /locus_tag="ACHKTW_003244"
     CDS             10667..11386
                     /locus_tag="ACHKTW_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein I"
                     /protein_id="HFO6464213.1"
                     /translation="MSKTVLPPSASVFLKTVDRSGEKISQLPVKLNTLWNADECPEVL
                     LPWLAWTLSVDRWDKAWTEETRRDVIRESWMVHRHKGTISAMRRAIAPFGALLSITEW
                     WENGDPPGSFRLNIDVTHAALTEATYQEMERMIALAKPVSRHLKALRLTYIAKGEVFT
                     GAVSHSGDIIQVYPDATLPNPCITGGEIYCAGAIYDSDITRVKPDTEIPSPCAAQAAI
                     CIAGTTYDGTTTIVKPAPEEP"
     gene            11383..12972
                     /locus_tag="ACHKTW_003245"
     CDS             11383..12972
                     /locus_tag="ACHKTW_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO6464214.1"
                     /translation="MTQKYKTIITTEGRRKLAEALKPGGKKVTLAKMQVGDGNGQEVE
                     PSEGQKQLVKKVWEGNISSITQDAENESFVTAELVIPADQGGFWVREIGLFDDEGALI
                     AVGNVAEGYKPKLEEGSGREQVFRMVILLSNISALNIKLDQSVVMVTKAALDQAIKEH
                     EKSGTHQSASTSTKGFVQLSSATDSDSEETAATSKAIKTLKGMINEKSSVTDATTSQK
                     GIVRLSSETGSAAENAAATPKAVQLTMQKATEAEKLAEQAKQSAQAASEKASTLEQQI
                     NATNQTVQQLQQSTQSGAQKAKYTALTVDLNTLGKPEHHGDYYQSANKDATTAMHYPV
                     EQAGYLSVKMGAWGFCQQEYTTWNPIRKFVRAVNGNFTGNGPWTDWKELGAEGPAGRQ
                     GPAGPTGPTGPTGPQGNTGPRGLQGETGRQGPTGETGPQGPTGRLVPGEIYSIGTYII
                     AVLGPYITDIGRTYQPGETVSGSRLKRCALIKDENGNYMKANTDGIDGKLSSVPGSWM
                     VCNEITSTNDNESIGLFQRAY"
     gene            12989..13591
                     /locus_tag="ACHKTW_003246"
     CDS             12989..13591
                     /locus_tag="ACHKTW_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HFO6464215.1"
                     /translation="MNVEKISNPQWADKDHTAVNCMVKFEHIEQAVPFTATASDTEAY
                     GRDIYAACLRGEAGEIAEHVQPSISPEKARELKTAGINAWRNRMESASYIFSFNGRNW
                     DYGKETQDRLGPSVIAAKEGNLPEKFFWTDADNNDVPMTAEELIALSEAAQQAMFAKG
                     MEIHVRQRNMKKEIAELQDAEAIQRYVVGWPTTEEPATER"
     gene            complement(13627..14091)
                     /locus_tag="ACHKTW_003247"
     CDS             complement(13627..14091)
                     /locus_tag="ACHKTW_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO6464216.1"
                     /translation="MMMIYGMFVFQLSTLPHQQIQQSRNWRHVKNERINRSASWQYIG
                     AGDDTITLSGLLYPEITGGEVSLTALTSQAYVGRPWPLIDGVGQIYGMYVITGLNTTR
                     SELDRYGRARKIEFTVTFERVDEDLRERLQSSSLGDLANNVKGALNNLSIPV"
     gene            complement(14103..16934)
                     /locus_tag="ACHKTW_003248"
     CDS             complement(14103..16934)
                     /locus_tag="ACHKTW_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO6464217.1"
                     /translation="MSNNLQIRVALTAVDRLTQPVRRAREMTGALSESLSSTQNALKS
                     LQRSSDAFSRARQQVTSTENSLNEARARLQRLQEAQRTGTTLTEKQQEAIRSMTVKID
                     RLNTVYSLHKSRLSEASRELESHGIRADGSNRAVERAIRRTQEYNDQLERQRGQLARV
                     TQAQQRYDRVQDIAGKLSQRGAVAMAAGSAGLYAAGRAVAPVVEEQRQGALIAAQSGG
                     SAEDGQRYSRMIQSIRASGAATDIAMISEAAAAAQSTLGALGTVGTEELERVTRQAID
                     LAQVTGGNVAEQVQAAGIMLKNGLAANAGEAVDLMAAGMQKMSAEMRGELPEILHEYS
                     THFRNMGLSGSETMTLLVSMAQQGKFALDKTGDAVKEFSIRGSDMSKSSVEAYELIGL
                     NAERMSSAIASGGAAAREAMRRTASGLLQIQDPAERANAAIALFGTPIEDLAVDQIPD
                     FLRSLATVSDRMGLVQGTARRMGDVLRDNLGGDTDRLSGIFSGIRSDVVSLVTDDIRE
                     LVQAVASVGERFRSWVQENPQLVRTLTLTVGGVLAATTAIGALSVGGGLLTGIFAKLQ
                     LGSALLGGGLSSLTNGVGGLALRLSGLPALWGMVSGAVSVLGGVLSGLLSPVGLVIAA
                     LAGGALLIWRHWEQVKAFMLGTFRSLWAGLAPVRESIAQFAPLFDLVSGAVSTVFDWF
                     TRLLTPVQASNESLERCTSAGESFGRVLSGAISLALTPLKALMDGLAWVLEKLGVLPD
                     EAERARRKLEDAQRASLLQGKLSLLQGDVNAIISPAKPVDGNDKGNNGGKPSVINDGT
                     SGVLRRLKGIEGNTSGMLQEARKRIGPGDIVFKNLPRALAVRGQWNEPTVASGLPLMP
                     AFSPVSPAVSPDSSRPVEVKRWDPVSPTPRGPSRSPEPPSVYGGDIHVHLHNVVTQNP
                     RDLARMVGEAVREEIRRQSGGMNSFRDRD"
     gene            complement(16927..17052)
                     /locus_tag="ACHKTW_003249"
     CDS             complement(16927..17052)
                     /locus_tag="ACHKTW_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GpE family phage tail protein"
                     /protein_id="HFO6464218.1"
                     /translation="MVADIAVVFHWSPSEIFVMTPGELVAWRERAAVRTGNHNDE"
     gene            complement(17082..17462)
                     /locus_tag="ACHKTW_003250"
     CDS             complement(17082..17462)
                     /locus_tag="ACHKTW_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HFO6464219.1"
                     /translation="MDQTEENIVTNDTDAVTNGDTENAEAIANDTVNLRVPVVCGNKT
                     VSSVKIGALARQVGSLRGIRMTDLFNTDVDAAAIFLERVTEPHLSRAALMSMCPEDFF
                     KLYEVAVGFLLPSSAKKAQEAGGA"
     gene            complement(17514..18026)
                     /locus_tag="ACHKTW_003251"
     CDS             complement(17514..18026)
                     /locus_tag="ACHKTW_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020240509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail tube protein"
                     /protein_id="HFO6464220.1"
                     /translation="MAVPKKLRVFTVFVDGDNKLGKVTSFTPPKLTRKTESYRGAGMP
                     GSASVDLGLDDGALDLSFAVGGVDRHIFAKYAAGIDAVRVRFSGEYYSEEGIEYVDIE
                     ARGRIVEIDKGEAKQGEDTEHTYAMKCTWYKLSVDNSDVIEIDVLNFIWRIDGNDVLP
                     DRTRSMLGLG"
     gene            complement(18026..19219)
                     /locus_tag="ACHKTW_003252"
     CDS             complement(18026..19219)
                     /locus_tag="ACHKTW_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001751361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HFO6464221.1"
                     /translation="MAEKRFHGVRVTENTDLVTAINDVDSSVIGIVAVADDADTGQFP
                     LNKPVLITRVSSVLGKAGKAGTLYKALKAISDQVSTRVIVVRVEAAKEGAENKTQDQL
                     VIGTTEEDGSYSGMQALLVAEQMEGIGCRPRILAAPGLESDAVTAALVTVAGKMRAFA
                     YSGCPGCTKPSDAIAFRKKISGREIMLLWPDFIAFNPVSGKNETFPAAAYACGLRARL
                     DHEQGWHRSLSNMPVKNVLGVSASVSWSLQDENSDANTLNNSEVTTLIHQGDGSFRFW
                     GNRTPETAEYIFEVYTRTAQQLADTIAEAQLQIVDSPLTPANAKDAVSAIKAKLDALV
                     TAGKLIGAECWFDIVDNNTTSLRQGIVRIRYKYTPVPPMECMELYQTFTDEFFGSAFA
                     SLGGS"
     gene            19373..20518
                     /locus_tag="ACHKTW_003253"
     CDS             19373..20518
                     /locus_tag="ACHKTW_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage late control D family protein"
                     /protein_id="HFO6464222.1"
                     /translation="MSITESVTHHINSALDAAEDTVRVPDFTITLEGKDASLKNITQR
                     IMSLTMTDNRGFEADRVTLEIDDADGGVVLPERGTRLSLAMGWKGESLTRKGTFVVDE
                     VSHEGPPDKLTISANSADFREEFNVKREVSWHDVTVERVVSAIAHRYGLKAQISEMLM
                     NIEIDHADQTEESDISFLTRMADMLGAITTVKNGSLLFILPGGGVTASGRALPSFALT
                     RSDGDSHRFRIADRKAYTGVKAYWLDLKFGQKKAVNVKKRNAKTTQKPEKSSSREGGY
                     MEGAEGNVYVLRKTYPNEEAARRAAAARWQQLQRGAAEFSITLARGRADLYPEMHGTV
                     SGFKSDIDNQDWIISRIEHVIDDSGFTTRLELEAKIADWIAERATTG"
     gene            20560..>20844
                     /locus_tag="ACHKTW_003254"
     CDS             20560..>20844
                     /locus_tag="ACHKTW_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ogr/Delta-like zinc finger family protein"
                     /protein_id="HFO6464223.1"
                     /translation="MHPCPYCGASTRTRTSRDANDTGTIREKYYQCNNIECSATFVTH
                     EFVVRTVCRPQPTEERGVVPADAFKSCHRGRDQLSLPLGDCASQSKTVHYA"
BASE COUNT         5527 a         5665 c         5727 g         3925 t
ORIGIN      
        1 agaaggtcaa tttccttaaa atcgctgccg cttttctctg tttttgtggt gagctggatc
       61 aggcgcgcat caatggactg ggtgacccgc tggatcggag gtgtggcatc ccactcatcg
      121 cgttttttcc atgagtaaat cgtgttctgg ctgatcccca tcagttgcga gatttccgca
      181 ggtgtgtacc cttgccagta aagttgtctt gcccttatgc ggacaaatgc atcctgaatc
      241 attgacgccc ccttgctgtt tgatctcaag agtaaacgcc cgcgcattca tccaggtatc
      301 cggtgccatt gtcgccacgc tcttacaaca tcgccgcctt gaggcgcagc catcccctta
      361 tcatcatgaa gaaaagcaac ccacagaaag gggatttaca tggcaagtaa aaacgcgcca
      421 gtacgaaaaa aattccgcgt cgccgtctcg ggcacaacca tcgacggacg tgaaatttca
      481 ggccagatgt tgcgtcaggc cgcagagaac tacgacccgg aagtgtgggg gtcacgggtg
      541 aacgtggagc atatgctttc acgtatgcca tccagtgaat tcagtgctgt gggcgatgtg
      601 atttcactgt ccaccgaaga aatcagggaa ggcaaactgg ccggccgcac cgcactgtat
      661 gccgaaattg agcccaccga ccgcatgacg cagatgctga atgatggcaa gaaaatctac
      721 tcaagcattg agcttgaacc caacattgat gccgttggcg gcccttacgt aattggcctg
      781 gcaatgaccg ataccccggc aagtctcggc actgaacgac tgaagtttgc cgcccagcag
      841 cgcgccagca tcatgcagtt caacagccgc agcggtgagc cagtcatgtt caccgaatgt
      901 atggaagcgg aactcgcggc ctccgtacag gacagcaccg aagaaagcca gaaatggttt
      961 tcgcgcgtca tggcgctcat cagcaaaacc cgtgacacgg acagcgagca gttcgctcac
     1021 gtgcgcgaag ccgtcgagag cattgccacg gaacaggccg gccttctgga tcgcttccat
     1081 gcgatggaag agcaacagcg cagtgataag gcatcaatgg aagccctgac ccgcgaactg
     1141 actgaactga aggcgcagct gaaaaccgaa gacgcagaca aggaacagcg ctttgcttca
     1201 accggtggca acagccacgc cctggctgat ttttaacaga ggaacaaaat catgggaaac
     1261 cgcttaacgc tttctcgcga aggccgcagc aatcttgagg cctatcttgc acgccaggca
     1321 gaactggccg aaaccaccgt agaacgactg gggaaaacct tcagcgttga tccggctgtt
     1381 catcagaaga tggaaaacgc catcaaagag agcgacgagc tgctgaaaag aatcaacagc
     1441 atcggcgttg acgatcagga gggtgaaaaa gtcctggtga ataccagcgg ccccatcgcc
     1501 agcaccaact caaccagtga cggcgtgaag cgacgcaatc ccgctgacgt ctctgatctg
     1561 gcatcgcgtc gttatcgctg cgaacaggtg aactacgaca ctttcatcag ctacgcacag
     1621 attgacgcgt ggagcaacca gaaaaacttc cagcaactgc tgagtgcaca aatcacccga
     1681 cagattgcac tggaccgtat catgattggc tttaacggtg agtcgcacgc catcatctcc
     1741 gatcgttctg ccaacccgaa acttcaggac gttaacaccg ggtggctgaa acacatccgt
     1801 gaacacgcca cgacgcgtgt cgtgaaggca atgacgctga cccgtcgcga tcatgaaaac
     1861 aaactggttg caaaaggtga ctacggcaac gctgacgcga tggttaacga cattcgttct
     1921 tccgtactgg atgagtggca caaggacgcc ccggatctgg ttgttgtcat gggccgcaac
     1981 ctgttcaaca cgctgcgtct gcctatgatt aacgccatca gcaccaccaa cccgaacacg
     2041 gagctggtcg ccggacagct gattacagcc tcccgcacca ttggcgcgct gccgaccttc
     2101 tttgcgccgt acttcccgga cgatgccatg ctgatcacgt cgttcagcaa cctttcggtg
     2161 tacttccaga aagggtcact gcgtcgcttc atccgtgaag agccggagta caaccgtatc
     2221 gccacctacc agtccatgaa cgactgctac gtggtggaag actacggcaa gtgcgcactg
     2281 attgagggca tttctttcgc cggtgaagat ccggaagaca agaaagaggc agaagcacag
     2341 gcgtaacgca tcatggcggg cacatgtgcc cgccggttaa tcaggagtcg aatacatgct
     2401 gacaccggca caacgatacc ttcagcgcgc gatggcgcat catcgggctg atccattcaa
     2461 tcgcgaagag cagcgaacac gaacggcaca tgaacaggtt cttcaccagt tacgcctcgc
     2521 acagttgcag ctgaatggcg tgcagtcaaa catcgcgaaa gcggagctga agaagcacct
     2581 gataccgaat tttgaaggct ggatcgacgg cacactggca ggcgacagcg gacgccagga
     2641 cgaagtcatt accaccatga tggtgtgggc aattgactgc cgggattatc cgctggcact
     2701 gcgtatcggt gagtacgtca ttcgtcaccg gctggcactg ccggacaact tcgggcgcga
     2761 ttcggcgacc gtgctcaccg aagaaattgc cgaagttgtg ctgacagaag ccgccaccaa
     2821 cagcgaaacc gacttatccg gctatatccg tatgctggac gagctggatg cactggttca
     2881 cgaccaggac atgccggatc aggttcgtgc aaaactacat aaggcgcggg cattttcacg
     2941 gcgcgcatca ggcagtacgg aagacatgac tgtgtcactt ggtctgctgc gtgaagccat
     3001 gcgcatcaat ccggcagcgg gcgtgaagcg tgaaatcgcc acgctggcca gaaaggtatc
     3061 aaaggttcag ccggaaccgg aaaaaccggc agaagagaaa agtaaatcga aagcaacatc
     3121 agggcgcaaa aagccgacta caaaagcgac ggccagaaaa gccacgaaaa aaaccgccgc
     3181 aaaaccggcg cgcaaggcga cctgataaca ccggacccga cctcgtcggc aggcggcgcg
     3241 accggtgatc tgatcgctga ctcccggtca ttttcaccgg tcgcccaccg cctgatctac
     3301 aggagaagac atcatgagca tggtagccag accccgtgtg aacgacgcca cggatgacac
     3361 gacagacacc gacgacggag aagcgcgcat cagtacagac gcgttctggc cggatatcgt
     3421 gctgaaagag ctgcgccttg ccatgcgcat tcccgggcgc accaccacct cacgtctgct
     3481 gcattcggcc accgaagctg tggcacatgt aacggcagag cttgacgcct ggaaacagaa
     3541 acaggaggcg gcgggacggt cgacactggc ggacgttccg gcagcacaca tcaacggtga
     3601 gagcgtgaac gtgcaccggt atcgacgcgc ggtgtatgca ctgacccgcg cgggcgttct
     3661 tgagcgttcg cgggatgtgg acaccaccga caaaggtgat cgccgggccg atgcgctgga
     3721 catgcagatt gaagatatgt ggcgcgatgc acgctgggca atatccgata ttcgcggcgt
     3781 cagacggatt tatgcggagc tgatttaatg aaggtaaccg cgcatgacgg cgacaccgtt
     3841 gacttactct gccagcgtca ttacggcatg acaaagggcg tgacagagca cgttctggct
     3901 gccaatccgg ggctgagtaa cacggtattc ctcactgccg ggcaggaagt tgaattaccg
     3961 gaaatcacac aggaaaaaca gcgcgaaacc gttcagttgt gggattgatt atgtttcaga
     4021 gaatttacga ccacattacg tttttcacat ccgtcatggt caccggtata ggggttatga
     4081 cgctgagcga gaagatggcg ctggcaggac tgattctggg ggcgctttca ctgctgcgcg
     4141 gatggatgca cagaacacgt atggagcagg cacaggagcg gcgcaatgag cttattgcac
     4201 gcattgccga ccagatgcag caccgaccac taaaaggcag cgagaagcgc gctctggaag
     4261 ccctgaatgc agacggggaa tcacatgcca ggacagatta aacgctatgc cgttgccgcc
     4321 atcgtggcgc tgggtttttc gctggccccc ggggagctga gaacctcaca ggaggcacag
     4381 gaaaaaattg ccacctggga agagtgtcgg gcattcccgt acagggatat cgctggcgtg
     4441 aaaacagtgg gttgtggctc aacgggaaac gtccccgacc gccaccatac caaacaggaa
     4501 gttgccggtc gatgggtgaa cgatttacga cgggcggaga actgcatcaa tcagaatttc
     4561 aatggccgca acatgccaca gcgccacttt gaggcaatga cggatgctgc ctttaatctg
     4621 gggtgcaaag gcctgatgtg gtttaagggg cgtgacggga ggatgcacag aacaaccatc
     4681 tggaagttcg ctcagggaaa gaactggagc gcaatgtgcg gcaggctgac ggattttgtg
     4741 aacagcggcg gacgctattc acaggggctg tttaaccggc gcaatgactt caggaactgg
     4801 tgtatgaacg gagtgaagca atgaaaacac ctgccgtgtt gctctggttt cttgccaccc
     4861 tgttgctttg tggcctgaca ggctgggaag cataccgcaa agggaaggaa agtctcggac
     4921 gggaacagat gatggacatc atagaggaca accgcgccgc catgcaggaa gttcgccatg
     4981 catacacaga cttttccagg gtgataaacg tgatccgcat tctggaccag caacggatca
     5041 cagaaggaga gcaacgacgt gaagaaatgc aaaaagccgt gcagggcgac acgtgtgcca
     5101 gcgcttatcc tcccgctgct gttactgagc ggttgcagca acacgccgca cacgttgaaa
     5161 accagactcg tttacgagcc ggtgccgaca gttctgacaa ggcagacccc ggttccggca
     5221 ctggaaatac ccgtgacatg gtacagggtg actgaatgga gcgatcggct actggaagca
     5281 ctcgacacat gcaacgcaga caaggctgcg atcgcctggc tggataaaca aagagcagaa
     5341 cgggcgcaag atgcgcccca tcaggagtaa acgatgagca aaaaacactt tgaactgatt
     5401 actgccacca accgcgcaga attcgctgaa aaagttaaca agcggttaca ggagggctgg
     5461 gaacttctcg gtaatgccgg tattgcatca gagccgggag tcccctggta tctcatgcag
     5521 gctatggttt caggcagtga tgaccaggga ggcaccatta tcaccccgac atccgacagc
     5581 gaaccggagt ggtattacgt tgtggtgagc gctggccagt ccaactctat ggcatacggt
     5641 gaggggttac ctctcccgga aagttacgat aaacccgatt cgcgcattcg ccagctggca
     5701 cgccgcagca ccgtaacccc gagcggaaaa gcttgtgcct ataacgacat cattctggcg
     5761 gatcactgcc tgcatgacgt gcaggatatg agccagtaca accacccgaa agccgacctg
     5821 aacaaaggtc agtatggctg tgtgagtcag ggattgcaca ttgcgaagcg cctgctgcca
     5881 tttatcccgg caaatgcggg gatccttctg gtcccatgca gccgtggcgg ttccggcttt
     5941 acaaccggtg atgcggggca attcagcgag atcggcggcg caactgaaaa atcctgccgc
     6001 tggggaacga acacgccgct gtacaaggat ttaatcagcc gcacaaaagc agcgctggcg
     6061 aaaaatccga aaaacgtcct gctggccgtc tgctggacac agggcgaggc agatcttgag
     6121 aaagaacaaa acgccgcaca gcataaagat cttttcacag ccatggtgaa gcagttccgg
     6181 gctgacctgg ccgatgttgc gaagcagtgc aaccagggca accctgaaag cgtgccgtgg
     6241 atttgtggag ataccaccta ccactggaaa aaaacctacg aagcacagta cgcaaccgtg
     6301 tatggcggat acaaaaacag cactgagaag aatattgttt ttgtgccttt tgttacggat
     6361 gagtacggga aaaacacccc aacaaacgag ccgacagaag atccggatat tgcggaagcg
     6421 ggttattacg gtgccgcatc ccgcagtagc ggaaactggg taagccatct gcgtggaacg
     6481 cacttcagct catgggcccg ccggggcatt attgccgatc gcatggcgtc agcaatcctg
     6541 cttaacgtcg gccgcacgct gccatttatt gcgggtacag agccggaacg cgtggcaaaa
     6601 ccatcacagg atgcaccggg cggcatcagt aagccatctt caccggaagc agaacaaccg
     6661 gcagtcagac cggggccggt cacgctcatg agtctgctgg catcatctcc tgaactggaa
     6721 agccagggct ggacgctgag cgaaggcaaa aacggggcca acacaccaaa acaactgaaa
     6781 gacatgctga tcggtgatga ggaagcaaca gggaaaaaag ccctgaaact gaccaaacag
     6841 gaaggcaata cctggtatct ggagcataag gccggtaatg gtgctgacct tctgaaaaat
     6901 ggcgggactg taagttgccg attcaaaatc gagggtgagc tgaaaaaaga ccagtacgca
     6961 atggcgatgt actggaaaac atcagcatta ccgcaggatg tgaaattact gggggcagat
     7021 ccaaataatt tattgctctc attctttctt cagacagaca gcgcaaacct gaatctgatg
     7081 tatcacaaaa cgccaaacga acaactggga acctttggtc agttcaataa caactggcac
     7141 accatagcgt ttcgttttgc gggaaacaac agcacccagg taacaccggt tctggatggc
     7201 gtggacggaa aaccgttcat gctttccaca tcaaaagccg atgttaccaa cgacacgtta
     7261 cgcgtgaccg atatcacgta tcggccgacc tataccgtac tgattgacag cctgagcgtt
     7321 gtcgtaaatc agccaccggc ataacaatgc ttcccagcca tccggcgggg aagctcgctg
     7381 acaaccggga aaataaaagg agcaagccat gggatttatt catcagataa tgttgtattt
     7441 cagtaccgcc gtatcccttc tgtatctgat ctcaggggga tataaagccg tccgcaattt
     7501 ctggaacaaa aagatagatg aagcagccgc caggaaatca gctggcggag cggtcaacgg
     7561 ggtattgcca taacaacagg cacagcgcat cagaacggca ccactgtcca cagcaaaaga
     7621 aacaaaaaac cctccggtat acggagggtg caagagggga aaggtgagca agaaagaacc
     7681 cgaattaaaa gccatttaat tttgagattt ttaattgcca cacgcaacac gttctacggt
     7741 gttttatgct gaaactgaat tcattacgcg aagctctgac cagcaactgc cggtggtgta
     7801 aggccagccc ggaaaaattc acggtattca ttgaatcggg tggcattgaa accaccggtg
     7861 aatccccctc attcctgtac cgctacaacc tggtgttgtt cgtcatggac ttcacagaaa
     7921 gtatcgacaa catcatgctg ccggtcatgg cgtggctgta caggaatcag ccggatcttc
     7981 ttctcaatcc ggagaagaac aaaagcatca aattttcgac ggccatcaac gatgacgaca
     8041 gtgccgatat cctgctggaa atcccggtat gggagcgcgt gattgtgcgc cgtaatgacg
     8101 acggcacggt gacggcagag cacatcggtg agcccccgat aagccgggca ctggaagcat
     8161 ggcgcgtagt gtttgaagac atgacgtggc gaaccggaac ggagaccagc acatgagagc
     8221 ggaccgcgaa ctggatgtca tctttcagga tattctttcc ggcctgtcgg cagccggaat
     8281 tcagcgcacg gcaatgagcg tgggtaaggt cgtccgacag ctacagacaa aacgcatcag
     8341 aagccaggta tccccggaag gccagccatg ggagaaacga aaacgccgca tcatgcgcat
     8401 acaaaaaggc gtgaagttta tctggaagga cggccaggta agaaacctga aaaactggag
     8461 cacgtcacag ggatgtttcg ggcagaaaat caccggctac gatacagacg ccggggggat
     8521 caggacgttt tacaaggcag acatcgggga ctacctggaa attaacacac agcgcgtcgc
     8581 agcaagagca tcacgtcgct cacctatgtt cgaaaagctg agaacagcac gctttctgag
     8641 aatcatcccc gacacaaacg gcgtaagtgt cggctttcag ggccgggccg cgcgtattgc
     8701 cagagtgcac cagttcggtg aggtgggcct ggtatcaccc gggaacgcca cgaaataccc
     8761 cgtgcgcgag ctgctgggcc tgaacgaaaa agacaaacag caaatcacag aaaccgtgat
     8821 tgccggactg gggaaagcag cgaaatgaac atcgagctta tgcgtctgct ggaaaacatt
     8881 gtgcggattg gtgtggttac ggaaatcaac accgacacct gggaggtgcg tgtgcagacc
     8941 ggcggcctga ccacaaactg gatccgatgg aacagcgggc gggccggaaa atttaaaatc
     9001 tggatccccc catcaccgga cgaacaggtg tttctcctat gcccgggcgg caatccggaa
     9061 accgcagtca gcgcaggaag tctgtacagc aacgacaacc cgccaccggg atcgctggaa
     9121 aacgaaatgg tcattaccgc gccggacggt gcggaatttc gctataacgc acaggaaagc
     9181 agcctgacgg ccagaggtat aaaaaccgca accattacgg ctgaaaccag tatcacgctg
     9241 gacgcgccaa aggtggaatg tacgcagttg ctgaagacaa agacattcga gctgaccagc
     9301 ggcggaacaa tgaaaggcaa cgtgaaacac agtggcggca gcctcgaatc aaacggcatc
     9361 accgtacaca cccacgtaca cagcggagtg aagagcggta gcgatacgag cggaggccca
     9421 caatgagtac cggctacaca ggcatgaatc acgcaggcta cggaacgctg acagacatcg
     9481 accatctgaa ccagtcaatc acggatatta tcctgacacc ggaaggttcg cgtgttatgc
     9541 ggcgcgagta tggcagcaac ctgcccgctc ttcttgacag tccggcaaat gaaataacca
     9601 gactgcgctg tatgagtgcc gccgtgatcg ccattacacg atgggagcca cgcgttgcca
     9661 taaacagcgt ggacctgacg tggagaaaca atggcagcgc agtgttatcc atgagctatc
     9721 tgattgtaag caccatgcag agcgccagta tttccgttga tatacaggga gggaaggatg
     9781 ccgacaattg atttatccca gctgccgcaa ccggcgatca ttgaatcgct ggattatgaa
     9841 gtgattctgg cacagacaaa gacattcatg attagcgtct ttccggaagc catgcgcgat
     9901 gaagtggcgg cagcccttga actggagtcc gaaccactga acatcatcgc tcaggctatg
     9961 gcatggcggg agctgttact gcggcagcgt atcaatgatg gcgcagcggc ctgtatgctg
    10021 agtcattcat caggaagcga tcttgacaat ctgggtgtaa acatgaatgt gcagcgcctg
    10081 gttatcaccg aagcaacgga gaccacaccg gcggtaatgg aaagtgacgc cgcgttccgg
    10141 atgcgcatac agagcgcctt tgaagggatg agtgttgccg gaccttccgg ctcatatgag
    10201 tattttgcac gcagcgcaag cggaaaagtg gcagatgcca gagcaaccag cccggcaccg
    10261 gcggaagttg tggttgcgct cctttcagcc gacggagacg gaacggcgaa cgaagcactg
    10321 attaacgaag tgaaagccgt actgaacgac gaaacagttc gccccgtggc ggacagactg
    10381 acggtgaaaa gcgcggaaat catcagctac aagattgatg cgacactcta tttttacccg
    10441 gggccggaat cagaacccat acttcaggcc gcacgggcac aactggaggc gtggctggca
    10501 gagcagcgaa gaattggtcg tgacgttgcc cgttcagcca tcatggcggc cctgcatgtg
    10561 cccggagttc agcgggtgga gttaacacaa ccagcgaagg atctggtgat cagcgaaaca
    10621 caggcggcgc gatgctccgc cacagagatt cgcgccgggg ggaccgatga gtaaaacggt
    10681 gcttccgccg tctgccagtg tttttctgaa aaccgttgat cgctccgggg aaaaaatcag
    10741 ccagctgccg gtgaaactta acaccctgtg gaatgcggac gaatgccctg aagtactgct
    10801 gccatggctc gcatggacgc tttcagtgga cagatgggat aaggcatgga cggaagagac
    10861 tcgccgggac gttatacgcg agtcgtggat ggttcaccgt cataagggga ccatcagcgc
    10921 catgagaaga gctattgccc cgttcggcgc gctgctgagc attacagagt ggtgggaaaa
    10981 cggcgaccct ccaggcagtt ttcgtctcaa catcgatgtt acacatgcag ccctgacaga
    11041 ggcgacctat caggaaatgg agcgcatgat tgcgctggcg aagccggtaa gccgtcacct
    11101 gaaagcactg aggctgacat acattgcaaa aggggaagtc tttaccgggg cagtgagtca
    11161 tagcggagac atcatacagg tatatccgga cgcaacactg ccgaacccct gcattaccgg
    11221 aggagaaatt tattgtgctg gcgcgattta cgacagtgac ataacccggg ttaaaccgga
    11281 tacggagata ccgtcaccgt gcgccgcaca agccgctatc tgtatagccg gaacaacata
    11341 cgacggcaca acgaccatcg taaaaccggc accggaggaa ccatgacaca gaaatacaaa
    11401 accattatca ccactgaggg ccgccggaag ctggccgaag ccctgaagcc cggtggaaaa
    11461 aaagtcaccc tggcaaagat gcaggtgggc gacggcaacg gccaggaagt ggagccatca
    11521 gaagggcaaa aacaactggt gaaaaaagtc tgggaaggga atatctccag catcacacag
    11581 gatgcggaga atgaaagctt tgtcaccgca gagctggtta ttcctgctga ccagggcggc
    11641 ttctgggtga gggagattgg gttattcgat gatgaaggtg ccctgattgc tgttggcaac
    11701 gtagcagaag gttacaaacc gaagctggag gaaggttcag gtcgggagca ggtattcagg
    11761 atggttattc tcctgagtaa tatttctgcg ctgaatatta aactggatca gtcagttgtc
    11821 atggtcacaa aagccgctct ggatcaggcc atcaaagaac atgaaaaaag cggcacccac
    11881 cagtctgcat ccacaagcac aaagggattt gtgcaactga gcagtgccac cgacagtgac
    11941 agcgaagaaa cagccgcaac ctctaaggcc atcaaaacgc tgaaaggaat gattaatgaa
    12001 aaatcttcag tcactgacgc cacgaccagc cagaaaggca tcgtccggct gagcagcgaa
    12061 acgggcagcg ccgccgaaaa cgccgctgca acaccaaagg cagtccagct caccatgcag
    12121 aaagccactg aagccgaaaa actggctgaa caggctaaac agagtgcaca ggcagcaagt
    12181 gagaaagcct caacactgga gcaacaaatc aatgccacaa accagaccgt acaacaactt
    12241 caacagtcaa cacaaagcgg agcacagaag gcgaaatata ccgcgctgac cgttgatctc
    12301 aatacactgg gaaaaccaga acaccacggg gattactatc agtcggccaa taaagatgca
    12361 accaccgcca tgcactaccc cgttgaacag gcgggatacc tgtccgtgaa aatgggagcc
    12421 tggggtttct gtcaacagga atacactaca tggaacccca tcaggaagtt tgtcagggct
    12481 gtcaacggta atttcaccgg aaatggtcca tggacggact ggaaggaact gggggccgag
    12541 gggccagcgg gtcgacaagg acctgccggg ccgacggggc cgacgggacc gacggggccg
    12601 caggggaaca ccggaccacg aggtttgcag ggagaaacag gaagacaggg cccgacaggt
    12661 gaaaccggac cacaagggcc aaccggaagg ctggtacccg gggaaattta cagcattgga
    12721 acatacatta ttgccgtact aggcccgtat ataaccgaca tcggcagaac ctatcagcca
    12781 ggagaaactg tatcgggttc acgccttaag cgatgcgccc taatcaaaga cgaaaacgga
    12841 aactatatga aagctaacac cgatggcatt gacggcaaac tatcctcagt tccgggctca
    12901 tggatggtgt gtaatgaaat tacgtcgaca aatgataatg aaagcatcgg gctgttccag
    12961 cgcgcatact gacagacagg agatgaacat gaatgtggaa aaaataagca atccgcaatg
    13021 ggcagataaa gatcacactg ctgtcaattg catggtgaag tttgagcaca ttgaacaggc
    13081 ggttcctttc actgcaacag ccagcgatac cgaagcatac gggcgggata tttatgccgc
    13141 ttgtctgaga ggagaggccg gtgaaattgc agaacatgta cagccatcca tcagcccgga
    13201 aaaagccaga gaactcaaaa ccgccgggat caacgcctgg cggaacagaa tggaatcagc
    13261 cagttatatt ttcagcttta atggccgtaa ctgggattat ggaaaggaaa cacaggatcg
    13321 ccttggccca tcagttatcg cagcaaaaga aggcaatctc ccggagaagt ttttctggac
    13381 agacgcagac aacaacgacg tccccatgac tgcggaagaa ctgattgcgc tgagtgaagc
    13441 cgcgcaacag gcaatgttcg cgaagggaat ggaaattcat gtgcgccagc gcaacatgaa
    13501 aaaagagatt gcagaacttc aggatgcgga ggccattcag cgttacgttg tgggctggcc
    13561 cacaacggaa gaacccgcca ccgaaagatg aacagcaaaa acgccctgac accagggcgt
    13621 tattatttaa accggaatgg acagattatt cagggcacct ttgacgttat tcgcaagatc
    13681 accaagcgac gaggactgaa gacgttcacg cagatcttca tccacacgct caaaagtgac
    13741 agtaaactca atctttcttg cccgaccata gcgatcaagt tcagagcggg tggtgttaag
    13801 cccggtaatg acatacatcc cgtaaatctg ccctacccca tcaatcagcg gccatggtcg
    13861 cccgacgtaa gcctggctgg tcagtgcggt cagggagact tcaccgcccg taatttccgg
    13921 gtagagcaaa cccgaaagcg tgatggtatc gtcacccgcg ccgatatact gccagctggc
    13981 tgagcggttg attcgctcgt tctttacatg cctccagtta cgggactgtt gtatttgttg
    14041 gtgtggcagt gtgcttaact gaaaaacaaa catgccgtaa atcatcatca taaccatcac
    14101 tcctaatcac gatcgcggaa actgttcata ccgccggatt gtctgcgaat ttcttcccgt
    14161 accgcctccc ccaccattct ggcaaggtcg cgagggttct gcgtaaccac gttgtgaagg
    14221 tgaacatgaa tatcaccacc gtacacagac ggcggttcag gagagcgcga aggcccccgg
    14281 ggtgtcgggg acaccggatc ccaccgctta acctccacag gacgggaaga atccggggaa
    14341 accgccggag acacaggcga aaacgctggc ataagcggaa gcccggatgc aacagtcggt
    14401 tcattccact gcccgcgaac agccagcgca cgggggagat ttttaaacac gatatcgcca
    14461 ggaccgatac gcttgcgggc ttcctgaagc atacccgacg tattaccctc gatacctttg
    14521 agcctgcgaa gaacgccact ggtgccgtcg tttatgacag aaggcttgcc accattatta
    14581 cctttatcat tgccatcaac tggcttagcg ggcgagataa tggcattaac atccccctga
    14641 agcagggaga gcttgccctg aagaagcgaa gcccgctgtg catcctccag tttccggcgc
    14701 gccctttcag cttcatcagg cagcacgcca agcttttcca gtacccaggc aagtccatcc
    14761 atcagcgcct tcagcggcgt aagcgcaagc gatatcgccc cactaagtac acgaccgaag
    14821 ctttctcccg cagacgtaca ccgttcaaga gattcgttgc tggcctgaac aggcgtgagt
    14881 aaccgggtaa accagtcaaa caccgtggac accgcacccg acaccagatc aaacaacggc
    14941 gcaaactgag caatactttc acgaacaggc gcaaggccag cccacagcga ccggaatgtg
    15001 ccgagcatga acgccttaac ctgttcccag tgacgccata tgagcagcgc cccaccggca
    15061 agagcagcaa tcacgagacc aacaggactg agaagaccgg aaagcacccc gcccagcaca
    15121 gaaaccgcac cggagaccat cccccacagt gccggaaggc cggacaggcg aagagcaaga
    15181 ccaccaacac cgtttgtaag cgaggatagc ccgccaccaa gaagcgcaga gccaagctga
    15241 agttttgcaa aaatccccgt cagtaaccca cctccgacag acaacgcacc aatggccgtt
    15301 gtggctgcaa gcacgccacc cacggtaagg gtcagcgtgc gtacaagctg cgggttttcc
    15361 tgaacccacg acctgaagcg ttcacccaca gaagcaacag cctgtacaag ctcgcggata
    15421 tcatcagtaa ccagcgacac aacatcactg cgaataccgg agaaaatgcc ggacagacga
    15481 tcggtgtcgc cgccgaggtt gtcacgcaga acatccccca ttcgccgggc ggttccctga
    15541 accagcccca tacggtcaga taccgttgca agcgaccgga gaaaatccgg gatctggtca
    15601 acagccagat cttcaatggg tgtgccgaac agcgctatcg ccgcattggc ccgttctgca
    15661 ggatcctgaa tctgtaacag acctgacgcg gtgcgacgca tcgcttcacg cgcggccgcg
    15721 ccgccactgg cgatcgctga cgacattctt tcagcgttaa ggccaatcag ctcataggct
    15781 tcaacgctgg atttggacat atccgagccg cgaatactga attctttgac agcatcgccg
    15841 gttttatcca gggcgaattt cccctgctgc gccatgctga caagcagcgt catggtttca
    15901 gagccggata gccccatatt gcggaaatgc gtggaatact catgcagaat ttcaggaagt
    15961 tcgccgcgca tttcggcaga cattttctgc atccctgccg ccatcaggtc gacggcctca
    16021 cctgcatttg cggcaagccc gtttttcagc ataatgccag cagcctgaac ctgttcagcc
    16081 acattaccgc cggtgacctg agcaagatcg attgcctgcc gtgtcaccct ttccagttct
    16141 tcagtcccta cggtacccag tgcgccaagc gtgctttgcg ccgcagctgc tgcttccgaa
    16201 atcatcgcga tatccgtggc cgcacctgac gcacggatgc tctgaatcat acggctgtag
    16261 cgctggccgt cttctgcgct gccgccactc tgtgccgcaa tgagcgcacc ctgccgttgc
    16321 tcttcaacaa caggtgcaac cgcgcgaccg gcagcataca gaccggcact gccagccgcc
    16381 atggcaacag caccgcgctg actgagctta ccggcaatat cctgaacacg gtcatagcgt
    16441 tgctgcgcct gcgtgacgcg tgcaagctgg ccccgctggc gttcgagctg atcgttgtat
    16501 tcctgcgtcc gcctgattgc cctctcgacc gcacggttac ttccgtccgc ccgtataccg
    16561 tgagattcca gttcgcgact ggcttcgcta agtcgggatt tgtggaggga ataaaccgta
    16621 ttaaggcgat caatcttcac agtcatcgag cggatagctt cctgctgttt ttccgttagt
    16681 gttgtgcctg tacgctgggc ctcctggagg cgctgaagtc gcgcacgcgc ctcgttcaga
    16741 ctgttttcag tcgaggtcac ctgctggcgg gcacgggaaa acgcatcact gcttcgctgg
    16801 agtgacttaa gagcgttctg cgtggaggaa agagattcgg aaagcgcgcc ggtcatttcc
    16861 cgggcgcgcc tgacgggttg agtgagacgg tcgaccgcag taagggctac acgtatctgg
    16921 agattattac tcatcgttat gattcccggt tctgacggcc gcccgttctc gccaggcaac
    16981 cagttcaccg ggagtcatca caaagatttc agagggcgac cagtgaaata caacggctat
    17041 atcagcgaca atatcttcaa cgtaatcaaa agcaagaagc gttacgcgcc gcccgcctcc
    17101 tgcgccttct tcgctgacga gggaagcaaa aaaccgactg ccacctcgta cagtttaaag
    17161 aagtcttccg ggcacattga cataagtgcc gcgcggctga ggtgaggttc agttacgcgt
    17221 tccagaaaaa tggcggcagc atcaacatcg gtgttgaaca ggtccgtcat gcggatccct
    17281 cgaagcgagc ccacctgacg cgcaagcgcc ccaatcttca cgctggaaac cgtcttatta
    17341 ccgcagacaa caggaaccct gagattaacc gtgtcatttg cgatcgcctc tgcgttttcc
    17401 gtgtcgccat tcgtcacagc atctgtgtca ttcgtcacga tattttcttc agtctgatcc
    17461 attatttttc tcccggggca acaggacgcc gcccctgtta tcagatttaa ctatcagcca
    17521 agccccagca tggagcgggt gcggtcagga agaacatcat taccatcaat gcgccagatg
    17581 aagttcagca cgtcgatttc aataacatcg ctgttatcaa cgctgagctt gtaccaggtg
    17641 catttcatcg cataggtatg ttcggtatct tcaccctgtt tggcctcgcc cttgtcgatt
    17701 tcgacaatgc gaccacgggc ttcaatatcg acgtactcaa tcccttcttc ggaatagtat
    17761 tcacccgaga agcgaacacg cacggcatca ataccggcgg cgtatttagc aaagatgtga
    17821 cgatcgacgc caccaaccgc aaaggagaga tccagtgcac catcatcaag accgagatcc
    17881 accgacgcag agccaggcat acccgccccg cgatacgact ctgttttgcg ggtcagtttt
    17941 ggcggggtaa aagacgtcac cttgcccagc ttgttgtcac cgtccacaaa gacggtaaac
    18001 acacgaagtt tttttggtac ggccattagc tacctccaag agatgcaaaa gcagagccaa
    18061 agaactcgtc ggtaaacgtc tgatagagtt ccatacattc catcggtggt acgggcgtgt
    18121 atttgtagcg aatacgcaca atgccctgac gaagactggt tgtattgttg tcgacaatat
    18181 cgaaccagca ttccgcccca atgagttttc cggctgtcac cagtgcatcc agctttgcct
    18241 tgatagcgct cacggcatcc ttagcattgg caggcgtaag cggtgaatcc actatctgaa
    18301 gttgcgcctc cgcaatggtg tcagccagtt gctgcgccgt acgtgtgtac acctcaaaga
    18361 tgtactccgc agtttccggc gtacggttac cccagaaacg gaaggagccg tcgccctggt
    18421 gaataagagt ggtcacctcg ctgttgttca gcgtgttggc atcgctgttt tcatcctgaa
    18481 gtgaccagga aacagacgct gacaccccaa gaacattttt cacaggcatg ttagaaaggg
    18541 agcgatgcca gccctgttca tggtcgagtc gggcacgaag accgcacgca tacgccgcag
    18601 ccgggaaggt ttcgttttta ccggacaccg ggttgaacgc aatgaaatca ggccacagaa
    18661 gcatgatttc gcgaccggaa atctttttac ggaacgcaat agcatcggac ggttttgtgc
    18721 agccagggca gccggaatac gcaaatgcgc gcatttttcc ggcaacagtc accagtgcag
    18781 ccgtgacagc atccgattca aggcccggag cggcaagaat gcggggtcgg caaccaatac
    18841 cttccatctg ttcggcaacc agaagcgcct gcataccgct gtagcttccg tcttcttccg
    18901 tggtaccaat gaccagttga tcctgtgttt tattctcagc cccctctttt gccgcctcca
    18961 cgcgaacgac aatcacccgt gtactgacct ggtcagatat cgctttaagg gccttataaa
    19021 gcgtgcccgc tttcccggcc ttccccagaa cagaagatac acgggtaatc agcactggct
    19081 tgttaagggg gaactgcccg gtatccgcat catctgcaac ggcaacaata ccaatgacgc
    19141 tggaatcaac gtcattaatg gccgtcacaa ggtcggtatt ctccgtgacg cgcacaccgt
    19201 gaaatcgttt ttcagccata cgattcacca tcagttgtgt ttatgctcgc ctgaataatc
    19261 caccattggc gcacagccat cacctgatgc gggctgtggg gattttttta caacagagcc
    19321 agacagaaac gacaaggtac gcgtgggatg atacgcacac gggagggata tcatgagcat
    19381 cactgaatca gtaacacatc acatcaacag cgcactggat gcagcggaag ataccgttag
    19441 ggtgccagac ttcactatca cactggaagg gaaagatgcc agcctgaaga acatcacgca
    19501 gcgcatcatg agcctgacca tgacagacaa caggggtttt gaggctgaca gggtgacact
    19561 ggaaatcgac gacgccgacg gcggggtggt acttccggaa cgcggaacac gattatcgct
    19621 ggcgatgggg tggaaaggtg aaagcctcac tcgcaagggg acgtttgtgg tggacgaagt
    19681 gagccacgaa ggtccgccgg ataagctgac catatccgcg aacagtgcgg attttcgcga
    19741 agagtttaac gtgaaacgcg aagtctcctg gcatgacgtg accgttgagc gtgtggtatc
    19801 tgccatcgcg caccgttacg gactgaaagc acaaatcagc gaaatgctga tgaatatcga
    19861 aatcgaccat gcagaccaga cagaggaaag cgacatctcg ttcctcacgc gtatggctga
    19921 catgctgggt gcaataacca ccgtaaaaaa cgggagcctg ctgttcattc tgccgggtgg
    19981 cggtgtgaca gccagcgggc gcgccctccc ctcattcgcc ctgacccgct cggatggcga
    20041 cagtcatcgc tttcgcattg ctgacagaaa agcctatacc ggagtgaaag cttactggct
    20101 ggatttaaag ttcggccaga agaaggccgt gaacgtgaaa aagcgaaacg cgaaaaccac
    20161 acagaagccg gagaaaagca gttcacggga gggtggatac atggaaggcg cagaaggcaa
    20221 tgtgtatgtg ctgagaaaaa cctacccgaa cgaagaagcc gcgcgtcgcg cagctgccgc
    20281 acgctggcag caacttcagc ggggtgcagc agaattcagc atcacgctgg cgcgcggacg
    20341 cgccgacctg tatccggaga tgcacggcac ggtatccggg ttcaagagcg acattgacaa
    20401 tcaggactgg attatttcgc gcattgagca cgtcatcgac gacagcggct tcaccaccag
    20461 actggagctg gaagcaaaaa tcgcagactg gatagcagag cgcgccacga ctggctagaa
    20521 tgagcgcgag cccaccccgt ttcagccagg gggaccatca tgcatccttg cccatactgc
    20581 ggagcaagta cccgcaccag aacaagccgc gacgccaacg atacaggaac aatcagggag
    20641 aaatattacc agtgcaacaa cattgaatgc agcgccacct ttgtgacgca cgagtttgtc
    20701 gtgagaaccg tatgccgtcc gcaacccacc gaagaacggg gagtcgtccc cgcagacgct
    20761 ttcaaatcat gccaccgggg aagggatcaa ctcagcctcc ccctgggcga ttgcgcctcc
    20821 cagtcaaaaa ctgtacatta cgcg
//
LOCUS       DAZFQD010000088        20774 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.088, whole genome shotgun sequence.
ACCESSION   DAZFQD010000088 DAZFQD010000000
VERSION     DAZFQD010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20774
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.088"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(171..2204)
                     /gene="betT"
                     /locus_tag="ACHKTW_003255"
     CDS             complement(171..2204)
                     /gene="betT"
                     /locus_tag="ACHKTW_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline BCCT transporter BetT"
                     /protein_id="HFO6464224.1"
                     /translation="MTDLSHSREKDKINPVVFYTSAGLILLFSLTTILFRDFSALWIG
                     RTLDWVSKTFGWYYLLAATLYIVFVVCIACSRFGSVKLGPEQSKPEFSLLSWAAMLFA
                     AGIGIDLMFFSVAEPVTQYMQPPEGAGQTIEAARQAMVWTLFHYGLTGWSMYALMGMA
                     LGYFSYRYNLPLTIRSALYPIFGKRINGPIGHSVDIAAVIGTIFGIATTLGIGVVQLN
                     YGLSVLFDIPDSMAAKAALIALSVIIATISVTSGVDKGIRVLSELNVALALGLILFVL
                     FMGDTSFLLNALVLNVGDYVNRFMGMTLNSFAFDRPVEWMNNWTLFFWAWWVAWSPFV
                     GLFLARISRGRTIRQFVLGTLIIPFTSTLLWLSVFGNSALYEIIHGGAAFAEEAMVHP
                     ERGFYSLLAQYPAFTFSASVATITGLLFYVTSADSGALVLGNFTSQLKDINSDAPGWL
                     RVFWSVAIGLLTLGMLMTNGISALQNTTVIMGLPFSFVIFFVMAGLYKSLKVEDYRRE
                     SANRDTAPRPLGLQDRLSWKKRLSRLMNYPGTRYTKQMMETVCYPAMEEVAQELRLRG
                     AYVELKSLPPEEGQQLGHLDLLVHMGEEQNFVYQIWPQQYSVPGFTYRARSGKSTYYR
                     LETFLLEGSQGNDLMDYSKEQVITDILDQYERHLNFIHLHREAPGHSVMFPDA"
     gene            2333..2920
                     /gene="betI"
                     /locus_tag="ACHKTW_003256"
     CDS             2333..2920
                     /gene="betI"
                     /locus_tag="ACHKTW_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308386.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BetI"
                     /protein_id="HFO6464225.1"
                     /translation="MPKLGMQSIRRRQLIDATLEAINEVGMHDATIAQIARRAGVSTG
                     IISHYFRDKNGLLEATMRDITSQLRDAVLNRLHALPQGSAEQRLQAIVGGNFDETQVS
                     SAAMKAWLAFWASSMHQPMLYRLQQVSSRRLLSNLVSEFRRELPREQAQEAGYGLAAL
                     IDGLWLRAALSGKPLDKPLAHSLTRHFITQHLPTD"
     gene            2934..4406
                     /gene="betB"
                     /locus_tag="ACHKTW_003257"
     CDS             2934..4406
                     /gene="betB"
                     /locus_tag="ACHKTW_003257"
                     /EC_number="1.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008802 - betaine-aldehyde dehydrogenase
                     (NAD+) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019285 - glycine betaine biosynthetic
                     process from choline [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="betaine-aldehyde dehydrogenase"
                     /protein_id="HFO6464226.1"
                     /translation="MSRMAEQQLYIHGGYTSATSGRTFETINPANGNVLATVQDAGRE
                     DVDRAVKSAQQGQKIWAAMTAMERSRILRRAVDILRERNDELAKLETLDTGKAYSETS
                     TVDIVTGADVLEYYAGLIPALEGSQIPLRETSFVYTRREPLGVVAGIGAWNYPIQIAL
                     WKSAPALAAGNAMIFKPSEVTPLTALKLAEIYSEAGLPDGVFNVLPGVGAETGQYLTE
                     HPGIAKVSFTGGVASGKKVMANSAASSLKEVTMELGGKSPLIVFDDADLDLAADIAMM
                     ANFFSSGQVCTNGTRVFVPAKCKAAFEQKILARVERIRAGDVFDPQTNFGPLVSFPHR
                     DNVLRYIAKGKEEGARVLCGGNVLKGDGFDNGAWVAPTVFTDCRDEMTIVREEIFGPV
                     MSLLTYESEDEVIRRANDTDYGLAAGIVTADLNRAHRVIHQLEAGICWINTWGESPAE
                     MPVGGYKHSGIGRENGVMTLQSYTQVKSIQVEMAKFQSIF"
     gene            4420..6090
                     /gene="betA"
                     /locus_tag="ACHKTW_003258"
     CDS             4420..6090
                     /gene="betA"
                     /locus_tag="ACHKTW_003258"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008812 - choline dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0019285 - glycine betaine biosynthetic
                     process from choline [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HFO6464227.1"
                     /translation="MQFDYIIIGAGSAGNVLATRLTEDPNTSVLLLEAGGPDYRFDFR
                     TQMPAALAFPLQGKRYNWAYETEPEPFMNNRRMECGRGKGLGGSSLINGMCYIRGNAL
                     DLDNWAQEPGLENWSYLDCLPYYRKAETRDVGENDYHGGDGPVSVTTSKPGVNPLFEA
                     MIEAGVQAGYPRTDDLNGYQQEGFGPMDRTVTPQGRRASTARGYLDQAKSRPNLTIRT
                     HAMTDHIIFDGKRAVGVEWLEGDSTIPTRATANKEVLLCAGAIASPQILQRSGVGNAE
                     LLAEFDIPLVHELPGVGENLQDHLEMYLQYECKEPVSLYPALQWWNQPKIGAEWLFGG
                     TGVGASNHFEAGGFIRSREEFAWPNIQYHFLPVAINYNGSNAVKEHGFQCHVGSMRSP
                     SRGHVRIKSRDPHQHPAILFNYMSHEQDWQEFRDAIRITREIMHQPALDQYRGREISP
                     GTECQTDEQLDEFVRNHAETAFHPCGTCKMGYDEMSVVDGEGRVHGLEGLRVVDASIM
                     PQIITGNLNATTIMIGEKIADMIRGQEALPRSTAGYFVANGMPVRAKK"
     gene            6303..6971
                     /locus_tag="ACHKTW_003259"
                     /pseudo
     CDS             6303..6971
                     /locus_tag="ACHKTW_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414844.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(7214..7909)
                     /locus_tag="ACHKTW_003260"
     CDS             complement(7214..7909)
                     /locus_tag="ACHKTW_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HFO6464228.1"
                     /translation="MDNRGEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLNQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGAENISQAEQAKVGVVYAEYGLTESGGVVLFSAAGRGRSLSLLP
                     ESSLFILRKSTILPCVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(7902..9329)
                     /locus_tag="ACHKTW_003261"
     CDS             complement(7902..9329)
                     /locus_tag="ACHKTW_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HFO6464229.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVANAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVWFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDSVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            complement(9340..10059)
                     /locus_tag="ACHKTW_003262"
     CDS             complement(9340..10059)
                     /locus_tag="ACHKTW_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HFO6464230.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAVKSYPTYLADEPEWASRAE
                     KVAVRMQDLTSFIVNKLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVVHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
     gene            complement(10586..11440)
                     /gene="rclR"
                     /locus_tag="ACHKTW_003263"
     CDS             complement(10586..11440)
                     /gene="rclR"
                     /locus_tag="ACHKTW_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine-specific transcriptional
                     regulator RclR"
                     /protein_id="HFO6464231.1"
                     /translation="MDALSRLLMLNAPQGTIDKNCVLGSDWQLPHGAGELSVIRWHAL
                     TQGAAKLEMPTGEIFTLRPGNVVLLPQNSAHRLSHVDNESTCIVCGTLRLQHSARYFL
                     TSLPETLFLAPVNHSVEYNWLREAIPFLQQESRSAMPGVDALCSQICATFFTLAVREW
                     IAQVNTEKNILSLLLHPRLGAVIQQMLEMPGHAWTVESLASIAHMSRASFAQLFRDVS
                     GTTPLAVLTKLRLQIAAQMFSREMLPVVVIAESVGYASESSFHKAFVREFGCTPGEYR
                     ERVRQLAP"
     gene            11666..12991
                     /gene="rclA"
                     /locus_tag="ACHKTW_003264"
     CDS             11666..12991
                     /gene="rclA"
                     /locus_tag="ACHKTW_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine resistance oxidoreductase
                     RclA"
                     /protein_id="HFO6464232.1"
                     /translation="MNKYQAVIIGFGKAGKTLAVTLAKAGWRVALIEQSNAMYGGTCI
                     NIGCIPTKTLVHDAQQHTDFVRAIQRKNEVVNFLRNKNFHNLADMPNIDVIDGQAEFI
                     NNHSLRVHRPEGNLEIHGEKIFINTGAQTVVPPIPGITTTPGVYDSTGLLNLKELPGH
                     LGILGGGYIGVEFASMFANFGSKVTILEAASLFLPREDRDIADNIATILRDQGVDIIL
                     NAHVERISHHENQVQVHSEHAQLAVDALLIASGRQPATASLHPENAGIAVNERGAIVV
                     DKQLHTTADNIWAMGDVTGGLQFTYISLDDYRIVRDELLGEGRRSTDDRKNVPYSVFM
                     TPPLSRVGMTEEQARESGADIQVVTLPVAAIPRARVMNDTRGVLKAIVDNKTQRILGA
                     SLLCVDSHEMINIVKMVMDAGLPYSILRDQIFTHPSMSESLNDLFSLVK"
     gene            13100..13336
                     /gene="rclB"
                     /locus_tag="ACHKTW_003265"
     CDS             13100..13336
                     /gene="rclB"
                     /locus_tag="ACHKTW_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine resistance periplasmic protein
                     RclB"
                     /protein_id="HFO6464233.1"
                     /translation="MFKKSVLFATLLSGVMAFSTNADDKIILKHISVSSVSASPTVLE
                     DAIADIARKYNASSWKVTSMRIDNNSTATAVLYK"
     gene            13348..13941
                     /gene="rclC"
                     /locus_tag="ACHKTW_003266"
     CDS             13348..13941
                     /gene="rclC"
                     /locus_tag="ACHKTW_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine species resistance protein
                     RclC"
                     /protein_id="HFO6464234.1"
                     /translation="MEKYLHMLSRGDKIGLTLIRLSIAIVFMWIGLLKFVPYEADSIT
                     PFVANSPLMSFFYEHPEDYKQYLTHEGEYKPEARAWQSANNTYGFSNGLGVVEVIIAL
                     LVLANPVNRWLGLLGGLMAFTTPLVTLSFLITTPEAWVPALGDAHHGFPYLSGAGRLV
                     LKDTLMLAGAVMIMADSAREILKQRSNESSSTLKTEY"
     gene            complement(14105..14971)
                     /locus_tag="ACHKTW_003267"
     CDS             complement(14105..14971)
                     /locus_tag="ACHKTW_003267"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFO6464235.1"
                     /translation="MEFSVLSNNLKMPMMGFGVFQVTDKNVCKQSVLNAIRTGYRLID
                     TAAVYGNEDAVGEAVREAISEGLCTRDELFITSKLWVQDMLNQDTAAAGIEASLKKSG
                     LEYFDLYLLHQAMRDYFSAWRALEDAYEEGKLKAIGVSNFYPHVLANFCETVRVKPMV
                     NQVELHPYFAQPEALATMKYYNVQPEAWAPLGGGRHKPFENNLLQSIADAHQKSISQV
                     ILRWNIQRGVVVIPKSTHQQRIEENFAIWDFSLTEKEMAQISSLDLGYVGESVKHFNP
                     EFVRGCLAVKNS"
     gene            15220..16077
                     /locus_tag="ACHKTW_003268"
     CDS             15220..16077
                     /locus_tag="ACHKTW_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO6464236.1"
                     /translation="MIRQKILQQLLEWIECNLEHPISIEDIAQKSGYSRRNIQLLFRN
                     FMHVPLGEYIRKRRLCRAAILVRLTAKSMLDIALSLHFDSQQSFSREFKKLFGCSPRE
                     YRHRDYWDLANIFPSFLIRQQQKTECRLVNFPETPIFGNSFKYDIEVSNKSPDEEVKL
                     RRHHLARCMNNFKTDIYFVSTFEPSTKSVDLLTVETFAGTVCKQTDSEMAKEWTINRG
                     LYASFRDEGEWERYPEWSRNLYLMELPARGLARVNGSDIERFYYNEDFVEKDSNDVVC
                     EIFIPVRPV"
     gene            complement(16199..20445)
                     /gene="fdeC"
                     /locus_tag="ACHKTW_003269"
                     /pseudo
     CDS             complement(16199..20445)
                     /gene="fdeC"
                     /locus_tag="ACHKTW_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000092554.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter adhesin FdeC"
BASE COUNT         5319 a         5351 c         4902 g         5202 t
ORIGIN      
        1 gctaatacaa aataaaatcg ttaattaata aaatataaag tcattaagtg atacaagagg
       61 aatttcagga tatgacatga caatcatata taatatatgc ataatttttt acagcgagtc
      121 aatgattccc gcgccatatg cagacatggc gcggttttat gcaaaaacaa tcacgcgtcc
      181 gggaacatca cgctatggcc cggcgcttca cgatggagat gaataaagtt aaggtgccgc
      241 tcgtactggt caagaatatc ggtgatcacc tgctctttgc tgtagtccat caggtcgttg
      301 ccctggctgc cttctaacag gaaggtttcc agccggtagt aggtcgattt accgctacgt
      361 gcgcggtagg taaagcccgg caccgaatat tgctgcggcc aaatctgata gacaaagttt
      421 tgttcttcgc ccatatgcac caacaaatcc agatgcccca actgttgtcc ctcttccggc
      481 ggcaggcttt ttagctccac gtacgcgccg cgcaaccgca gctcctgcgc cacttcttcc
      541 attgccgggt aacagaccgt ctccatcatc tgtttagtgt aacgcgtgcc cggataattc
      601 atcaggcgcg agagacgttt tttccagctc aggcgatcct gaagccccag cggtcgcggc
      661 gcggtatcgc ggttggcact ttcacggcgg taatcttcta ccttcagaga tttatacaac
      721 cccgccatca cgaagaagat cacaaagctg aacggcagcc ccataatcac cgtggtgttt
      781 tgcagcgcgg atatcccgtt ggtcatcagc atgccgagcg tcagcaggcc aatcgccacc
      841 gaccagaaga cgcgcagcca gccgggggcg tcgctgttga tatctttaag ctgcgaggtg
      901 aaattcccca gcaccagcgc accggagtcc gccgaggtca cataaaacag caggccagta
      961 atggtggcga cggaggcgct aaaggtaaac gccggatact gcgccagcag gctgtagaag
     1021 ccgcgctccg gatggaccat cgcttcctcg gcaaatgccg cgccgccgtg gatgatttca
     1081 tacagcgcgc tattgccgaa caccgagagc cataacagcg tggaggtaaa cggaataatc
     1141 aacgtgccca gcacgaactg gcgaatggta cgcccacgcg agatacgcgc caggaacaag
     1201 ccgacaaacg gcgaccatgc cacccaccat gcccagaaga agagcgtcca gttattcatc
     1261 cactcaactg gacggtcgaa ggcaaaactg ttgagcgtca tgcccataaa gcgattcaca
     1321 tagtcgccaa cattcagcac cagcgcatta agcaggaacg aggtgtcgcc cataaacaat
     1381 acgaacagga tcaatcccag cgccagcgca acgttaagtt ccgataacac acgaatcccc
     1441 ttatcgacac cggaggtgac agagatcgtg gcgattatca ccgacaaggc gatcagtgcc
     1501 gcttttgccg ccatcgaatc gggaatatca aacagtacgc tcaagccata gttaagctgc
     1561 accacaccga taccgagcgt agtggcaata ccgaagatgg tgccgatcac cgctgcaata
     1621 tccactgagt gccctatcgg cccgttaatc cgtttaccga agatcgggta cagcgccgag
     1681 cggatggtga gcggcaaatt ataacgatag ctaaagtatc cgagcgccat gcccatcagc
     1741 gcatacatcg accagccggt taagccgtag tgaaacagcg tccagaccat cgcctgacgc
     1801 gcggcctcaa tcgtctgtcc cgcgccttcc ggcggctgca tatactgcgt taccggttcg
     1861 gctacggaga agaacatcag gtcgataccg atcccggcag caaacagcat cgccgcccaa
     1921 ctcagcaggc tgaattccgg tttggattgt tctggcccga gcttcaccga accaaaacgc
     1981 gaacaagcga tacagaccac aaagacaata tagagcgttg ccgccagtag atagtaccaa
     2041 ccgaaggttt tagaaaccca gtccagcgtg cggccaatcc acagggccga gaagtcgcga
     2101 aacaggatcg ttgtcaggga aaacaacaaa atcagtccgg cggaggtgta aaacaccacc
     2161 ggattgattt tgtccttttc cctgctgtgt gaaaggtctg tcatccagta tccccactgt
     2221 tattgttacc atttaaaatc aaattcgtaa caattaagac acattttata ttgaacgtcc
     2281 aatcaataac cgctttaata gataaacaac gctgatgaat ggagtggcga aaatgcccaa
     2341 attggggatg cagtcgatcc ggcgcagaca actgatcgac gccacactgg aagcaataaa
     2401 tgaagtgggc atgcacgatg caacgatcgc gcagatcgcc cgccgtgcag gcgtttctac
     2461 ggggatcatc agccactatt tcagggacaa aaatggtctg ctggaagcaa ccatgcgcga
     2521 tatcaccagt cagctgcgtg acgcggtttt gaatcgatta catgcacttc cgcagggcag
     2581 tgcagagcag cgattacagg cgattgttgg cggaaacttc gatgaaacgc aggtgagcag
     2641 tgcggcgatg aaagcctggc tggcgttctg ggccagcagt atgcatcagc cgatgctcta
     2701 tcgtttacag caggtcagta gccgccgctt attgtcgaat ctggtgagcg aatttcgccg
     2761 cgagttaccg cgcgaacagg cacaggaagc gggctacggc cttgccgcgc tgattgacgg
     2821 attatggctg cgcgcggcac tgagcggcaa accgctggat aaacctctcg cacactcgct
     2881 gacccgtcac tttattactc agcatttacc caccgattaa ccgaggagac gtgatgtccc
     2941 gaatggcaga acagcagctt tatatacatg gtggttatac ctccgccacc agcggtcgca
     3001 ccttcgagac cattaacccg gccaacggta acgtgctggc gaccgtgcag gacgccgggc
     3061 gcgaggatgt cgatcgcgca gtgaagagtg cccaacaggg gcaaaaaatc tgggcggcga
     3121 tgaccgcgat ggagcgctcg cgcattctgc gtcgggccgt cgatattctg cgtgaacgca
     3181 atgacgaact cgcaaaactt gaaaccctcg acaccggaaa agcatattcg gaaacctcaa
     3241 ccgtcgatat cgttaccggt gcggacgtgc tggagtacta cgccgggctg atcccggcgc
     3301 tggaaggcag ccagatcccg ttgcgtgaga cgtcatttgt ttatacccgc cgcgaaccgc
     3361 tgggtgtggt ggcggggatt ggcgcatgga actacccgat ccagattgcc ctgtggaaat
     3421 ccgccccggc gctggcggca ggcaacgcaa tgattttcaa accgagcgaa gtcaccccgc
     3481 ttaccgcgtt aaagctggct gaaatttaca gcgaagcggg cctgccggac ggcgtattta
     3541 acgtgttgcc gggggtgggc gcggagaccg ggcaatatct gaccgagcat ccgggcattg
     3601 ccaaagtgtc atttaccggc ggtgtcgcca gcggcaaaaa agtgatggct aactcggcgg
     3661 cctcttccct gaaagaagtg accatggaac tgggcggtaa atcaccgctg atcgttttcg
     3721 acgatgcgga tctcgatctc gccgccgata tcgccatgat ggcgaacttc ttcagctccg
     3781 gtcaggtgtg taccaacggc acccgcgtct tcgttccggc gaaatgcaaa gccgcatttg
     3841 agcagaaaat tctggcgcgc gttgagcgca ttcgcgcggg cgacgttttc gatccgcaaa
     3901 ctaacttcgg cccgctggtc agcttcccgc atcgcgataa cgtgctgcgc tatatcgcca
     3961 aaggcaaaga ggaaggcgcg cgcgtactgt gcggcggcaa tgtactgaaa ggcgatggct
     4021 tcgataacgg cgcatgggtt gcaccgaccg tgttcaccga ttgccgcgac gaaatgacca
     4081 tcgtgcgcga agagatcttc ggtccggtga tgtcgcttct cacttatgaa tctgaggacg
     4141 aagtgattcg ccgcgccaat gataccgact acggcctggc ggcgggcatc gtgacggcgg
     4201 acctgaaccg cgcgcatcgc gtcattcatc agctggaagc gggtatttgc tggatcaaca
     4261 cctggggcga atccccggca gagatgcccg ttggcggcta caaacactcc ggcattggtc
     4321 gcgagaacgg cgtgatgacg ctccagagtt acacccaggt gaagtccatc caggttgaga
     4381 tggctaaatt ccagtccata ttctaaccag gaggtttatt tgcaatttga ctacatcatt
     4441 attggtgccg gctcagccgg caacgttctc gctacccgtc tgactgaaga tccgaatacc
     4501 tccgtgctgc tgcttgaagc gggcggcccg gactatcgct ttgacttccg cacccagatg
     4561 cccgcagcgc tggcgttccc actacagggc aaacgctaca actgggccta tgaaacagag
     4621 cctgaaccgt ttatgaataa ccgccgcatg gagtgcggac gcggtaaagg cctgggtgga
     4681 tcgtcgctga tcaacggcat gtgctacatc cgtggcaatg cgctggatct cgataactgg
     4741 gcgcaagaac ccggtctgga gaactggagt tatctcgact gcctgcccta ctaccgcaag
     4801 gccgagactc gcgatgtggg cgagaacgac taccacggtg gcgatggccc ggtgagcgtc
     4861 accacctcca aacccggcgt caatccgctg tttgaagcga tgattgaagc gggcgtgcag
     4921 gcaggctacc cgcgcacgga cgatctcaac ggctatcagc aggaaggttt tggcccgatg
     4981 gatcgcaccg tcacgccgca gggccgtcgc gccagtaccg cgcgcggtta tctcgatcag
     5041 gccaaatcgc gccctaacct gaccattcgt actcacgcta tgaccgatca catcattttt
     5101 gacggcaaac gcgcggtggg cgtcgaatgg ctggaaggcg acagcaccat cccaacccgc
     5161 gcaacggcca acaaagaagt gctgttatgt gcaggcgcga ttgcctcacc gcagatcctg
     5221 caacgctccg gcgtcggcaa cgctgaactg ctggcggagt ttgatattcc gctggtgcat
     5281 gaattacccg gcgtcggcga aaatcttcag gatcatctgg agatgtatct gcaatatgag
     5341 tgcaaagaac cggtttccct ctaccctgcc ctgcagtggt ggaaccaacc gaaaatcggt
     5401 gcggagtggc tgtttggcgg cactggcgtt ggtgccagca accactttga agcaggtgga
     5461 tttattcgca gccgtgagga atttgcgtgg ccgaatattc agtaccattt cctgccagta
     5521 gcgattaact ataacggctc gaatgcagtg aaagagcacg gtttccagtg ccacgtcggc
     5581 tcaatgcgct cgccaagccg tgggcatgtg cggattaaat cccgcgaccc gcaccagcat
     5641 ccggcgattc tgtttaacta catgtcgcac gagcaggact ggcaggagtt ccgcgacgca
     5701 attcgcatca cccgcgagat catgcatcaa cccgcgctgg atcagtatcg tggccgcgaa
     5761 atcagccccg gcacggaatg tcagacggat gaacagctcg atgagttcgt gcgtaaccac
     5821 gccgaaaccg ccttccatcc gtgcggtacc tgcaaaatgg gttacgacga gatgtccgtg
     5881 gttgacggcg aaggccgcgt acacgggtta gaaggcctgc gtgtggtgga tgcgtcgatt
     5941 atgccgcaga ttatcaccgg gaatttgaac gccacgacaa ttatgattgg cgagaaaata
     6001 gcggatatga ttcgtggaca ggaagcgctg ccgaggagca cggcgggata ttttgtggca
     6061 aatgggatgc cggtgagagc gaaaaaatga gtcgtgatgt gaactaacgc aggaaccgat
     6121 cagattgaga gttaccgttc cagagagggg gaccgaatcc ttatataaac actgaggtaa
     6181 ctctcatgca tcatatccag tattcatgat gctggctttt tgctgatatt taacccgaac
     6241 attcctgata cagactatta atacaacctt atctgcgaca gattttattt tctggatata
     6301 ttatgcgtga acaaatcaaa caggatatcg atctgattga gattttattt tatctgaaga
     6361 aaaaggttcg tgttatcctt tttattatag ctatctgtat ggcaatgatg ctgttgtttc
     6421 tgtatatcaa taaagataat ataaaagtga gttacagcct aaaaataaac cagacaacac
     6481 caggcatact tgttaactgt gatagcaata ataactttgc ctgtcagacc gcaatgactg
     6541 aagatgttat acagcgaatt actacatttt ttcacaccag cccagatgtc aagaacagag
     6601 aaataaagct ggaatggtca tgagataaga gagatttacc aactgctgaa gcggaaatat
     6661 ctcgcgtgta ggcgtctatt atcaaatggt atgcgtcaga ataccataat ggcaggcaag
     6721 ttctcgatga gatacaaata ccttcagcaa ttaacagtga gctttataca aaaatgatat
     6781 acctgaccag gaactggtca ttgtatccga acggtgatgg ctgtgtaact ataagctcac
     6841 cagaaataaa aaataaatac cctgctgcca tttgcctggc tctgggattt tttctaagca
     6901 ttgtaatttc tgtaatgttt tgccttgtca aaaaaatggt agatgaatac caacaaaact
     6961 ctgggcagta atacacagca ttctgataga gagtgcctgt ataaagctga agtgacaatg
     7021 accgggaagg gaacccgttt tcaggaatgg atgcctgtct ttttcactgt aaatatcctg
     7081 gcattactaa tgccaggata tttacagaag agcagcataa ccatgacgga acggggttaa
     7141 gtcaggttgt ccttaacgct attttcaacc tcgtatgcct tcttcaggtt tatgttcaga
     7201 tctcatatct ctctcagcaa tcctcaataa tcagatacac cgctttcacc gggccatgaa
     7261 ccccgacgac tttgataagc tcaatatccg ccgttgaact agggccgcta atgatgttaa
     7321 tgcaggacgg cattcgttca ccggcctgcg ctttctgatg caatttttct gcgagttgcg
     7381 ctacacacgg caggattgtg cttttacgca ggataaaaag agaagattcc gggagcaggc
     7441 tcaatgaacg cccgcgcccg gcggcggaaa aaagaaccac gcctcccgat tcggttaaac
     7501 catattcagc atacacgaca cccactttag cctgctcggc ctgcgagata ttctcggcac
     7561 ctttcgccgg atcccaaaca acggcattgc attcctgctg caaacgttca ctaatcccca
     7621 attcctccag cctcgtgtca ccgctaatca cgaccgactg atctcccagc tctttacaca
     7681 gacgtattgc agcttctgcc gccttcgcct cgctggtcag ctcacagcgc gtcaacataa
     7741 catcgctggc aaactgaata aacgcgtcac agcgctgctg ttggttaagt tgggtaagcc
     7801 gctcgttagc atagttgtta agcggcgcat cttctgcttg cggttcaagt cgcagcgggc
     7861 gacccagtgc ctgagcaacg ttattcaaaa attcgcctcg attatccatt ctttttctcc
     7921 tgcgcctgat gtttcttaaa ccaactacgg aaactctctc cgtcagcttc aggaagatcg
     7981 cgtgcttcca tccagtcgct aatcgcgcca aatttgagtg gtgttttgcc gccattgata
     8041 aaccagcttg ccgcatgagc gccggccatc atcccgactt tccacaatcc tggatgacta
     8101 ttggcataag cgaacatttt tatcgcccgt tgctctgctt ttgcggtgat ccctttttca
     8161 gccatcaccc gacgatgacg caaaatcagt tttgacagcg gaatacgcac cggacacacg
     8221 ctgtcacaag cagtgcataa agagcaggcg tagggtaaat ctttaaaatc tttatagccg
     8281 ccaagtagcg gagaaatcac cgcaccaatt ggccctggat aaatagagcc atatccatga
     8341 ccgccaatat ggcgatatgc cggacaagta ttcatacaag ccccgcagcg aatacagcgc
     8401 agcacatccc gaaattcaga ggccagcacc tcagaacgcc cgttatcgac aataaccaga
     8461 tgaaactctt caggaccatc aacgtgcccc gcttcgcgcg gtcctgtcag ccaggtgttg
     8521 tatcccgtca aacgtgcacc aacggcactg cgcgccagca tggtgatcaa tacatctacc
     8581 tcggcaaacg tgggggcaat acgctccatt cccatcactg caatatgcgt tttaggcagc
     8641 gtggtacaca ttcgcgcatt accttcattg gtcaccaggc ataccgaacc tgtctctgcc
     8701 accgcgaaat tacagccggt aatacctatt tcagcactga ggaaatcttc gcggattttt
     8761 tgccggatga ataaggtcat cgcttcaggc gtttccggcc cctcatagcc cagacgttcg
     8821 tgtagcactc gacggatctg atggcgatct ttatgaattg ccgggaccac aacatgagat
     8881 ggcggatctt gatccagctg gagaatatat tcacccagat cggtttcaat cacctgaatg
     8941 ccagcatcct gcaacacatg attgacccca atctcttcgg tcaccatcga tttagatttc
     9001 accaccttcc gggcattttt gcgttgggca acctgtaaaa tgtagcgggt agcgtcttct
     9061 ttggtttttg caaaccagac gtgaccgccg ttttgcgtca ctttttctga gagctggtac
     9121 agataagcgt cgagattact cagaacatga tcacgtatct gggcggcccg atcgcgccac
     9181 tcctcccagt gccccaattc atcgaccatt ttttgccgat ttgccccaat acgctgctgc
     9241 gcgtttgcca ccgctttgcg catgatcgga tcttcaattt gctgacggat gcgtgtctta
     9301 aaatctgtat tactggtttt gatcgacatc tttatatcct cagcggctca tcaacacttc
     9361 agcaatatgc atcactttga ctttctgccc ttcccgttgt aatcgcccac tgatgttcag
     9421 caggcaactc acgtcagcac caattaaata ctcagggcgg acttccatca ggtgcacaac
     9481 cttttctttc accatctcgc cggatatttc ggccattttg accgagaacg tgccgccaaa
     9541 tccgcagcag gtatcctgtt cagcaaaggt caacagctcc agtccacgca catttttcag
     9601 cagcgtaagt ggctcgtcct tcactcccag cttacgggcc aggctacaag atgggtgata
     9661 cacagctctc ccttgcaaac tggcacctac atcgactacc cctaatttat taacaataaa
     9721 agaggtgaga tcctgcatac gcacggcaac cttttcggca cgtgatgccc attcaggttc
     9781 atccgccaga tacgtcgggt aactttttac ggcataggtg caagagccag ccggtgaaat
     9841 aatgggatcg tcgttatcct ccagtgcggc gatcagattt ttcatccctg gaatcgcttc
     9901 tttgatataa ccgctattga tcgcaggctg accgcagcat ccctgtttct ccgggaaatt
     9961 tacgcgacag ccgagttttt ccagtagcag cacggagtct cgtgccattc ttgatttcag
    10021 ggcgtcacca atacaggtga caaagaaatt gacattcaca actaatactc cattacttca
    10081 tgcccattta tgcccgatgt ttccccccca ccgggggatc aatcatcaat aaatatggcg
    10141 ttctttttta attggctagc aacataccac ccctcatttg tatgacaaat gataatcttt
    10201 catccaaact tcaaatgcaa taactaattt tatttctttc ggcatcgctc acattttatg
    10261 gggaaatcga cgttcatttt tggcataaga aatccattta aaaaggctac atcccttata
    10321 cctattcaga gctacttatt caggggttat tgagatatac actgatggtg tacaaaaaaa
    10381 tcaccgacaa taatcacgta tcaagtaata gtcggggttt tatttaattg aagcaattta
    10441 gtttgcattt ttatttaaaa actgagctag agtccatcag aaaaaaatat ataaagccca
    10501 aaccaccgtt ggaaatagaa atagttgaaa ggctcacaac tcccggaatg cggattatct
    10561 ttgccctgat ttctggcggt tttattcagg gtgcaagctg tctgaccctt tcccgatatt
    10621 ctcccggagt acaaccaaac tcgcggacaa acgccttgtg aaaagatgat tcactggcat
    10681 agcctactga ctcagcgatc accacaacag ggagcatttc ccgggaaaac atctgggccg
    10741 ctatttgtag acgcaacttt gttaatacag ccagcggcgt ggttccggaa acatcacgga
    10801 aaagctgggc aaaacttgcc cgggacatgt gagcgatgct ggccagcgat tcgacggtcc
    10861 aggcgtgtcc tggcatttcc agcatttgct gtattaccgc accaagacgt ggatgcagaa
    10921 gcaaactgag aatgtttttc tcagtattaa cctgtgcaat ccactcacgc accgcgaggg
    10981 taaagaatgt agcgcagatc tggctacaca gtgcatccac tcccggcatc gccgatctgg
    11041 attcctgttg taaaaacggg atcgcctccc tcagccagtt atattccacg ctgtggttaa
    11101 ccggtgctaa aaacagcgtt tccggcaaag acgttaaaaa ataacgcgcc gaatgttgca
    11161 accgaagagt gccgcagaca atacaggtcg attcattatc gacatgactc aggcgatgag
    11221 ctgaattttg tggtagcagg accacatttc ccgggcgtaa tgtaaaaatc tctcccgtcg
    11281 gcatttccag cttcgccgct ccttgcgtta acgcatgcca acgaataacc gataattccc
    11341 cggcaccatg tggaagctgc cagtcacttc ctaacacgca attcttatcg atcgttcctt
    11401 gtggagcgtt aagcatcaac aaacggctaa gggcatccat atcagactcc tgagtggttt
    11461 ttgattacaa acaccaacaa tcttagtaaa taactaatct aataccatca aatggttaat
    11521 catgattttt gttttgtgat tctgattaag aaaaagaaaa taccagacga taagacaaaa
    11581 tatcaagcgt tgtgaagaaa tggtatttgc tcttttgcgt ctatagtcat gatgtcaaat
    11641 gaacgcgttt cgacaggaaa tcatcatgaa taaatatcag gcagttatta ttggttttgg
    11701 caaggctgga aaaacattag ccgtcacgct ggcaaaagca ggttggcgag tggctctcat
    11761 cgaacaatca aatgcaatgt atggcgggac ctgtattaat atcggctgca tcccaaccaa
    11821 aacattggtt catgacgcac agcagcacac agattttgtc cgtgccatac agcgtaaaaa
    11881 tgaagtggtt aattttttac gtaataagaa ttttcataat cttgcggata tgcccaatat
    11941 cgacgtgatc gacggccagg cggagtttat caataatcat agcctgcgtg ttcatcggcc
    12001 tgagggaaat ctggaaattc atggcgagaa aatttttatt aataccggtg cacaaaccgt
    12061 ggttccgcca attcctggaa ttaccaccac gcctggtgta tatgacagca ccggattact
    12121 taatctaaaa gaattgcctg ggcatttagg tattttgggc ggcggatata ttggcgttga
    12181 gttcgcctct atgttcgcta attttggcag caaggtaacc attttagaag cagcttcgct
    12241 gtttttgcct cgggaagatc gggatattgc tgataatatc gcgacgattt tacgcgatca
    12301 gggcgtcgat attatcctca atgcccatgt ggagcgaatc agtcaccatg aaaatcaagt
    12361 gcaagtgcat agcgagcacg cccaactggc ggtggatgca ctgttaatag cttccggtcg
    12421 tcaaccggct accgcttcgt tacatccaga aaatgctggt atcgcagtaa acgagcgcgg
    12481 ggcaattgtc gttgacaagc aattacatac caccgcagac aatatttggg cgatgggaga
    12541 tgttaccggc gggctgcaat ttacttatat ttcactggat gattaccgca ttgtgcgtga
    12601 tgagttgctg ggagaaggca gacgtagtac tgatgatcgg aaaaatgtgc cttattccgt
    12661 attcatgaca ccgccactgt ccagggttgg tatgacagaa gaacaggcca gagagagtgg
    12721 tgctgatatt caggtggtga cattgcctgt agctgcaatt ccgcgtgcca gagtgatgaa
    12781 tgatactcgt ggggtattaa aagcgattgt tgataataaa acccaacgta tattaggggc
    12841 atcactgctg tgtgttgact cccacgagat gatcaatata gtgaaaatgg tgatggatgc
    12901 cgggctgcct tatagcatat tacgcgatca gatatttact catccgtcga tgagcgaatc
    12961 actcaatgat ctattttcat tagtcaaata aactcaaaat cagacgccag aacaaatatt
    13021 ctggcgtctc agagaaaaga atcttattaa ttccctgtta ctctataact tcttaatcac
    13081 ttcattgatg gtattttata tgttcaaaaa atctgtttta tttgcaacac tattatctgg
    13141 cgttatggca ttttccacca atgcagatga taaaataatt ctgaaacata tcagcgtctc
    13201 gtcagtatca gcatcaccga cagttctgga ggatgccatt gctgatatag ccagaaaata
    13261 taatgcttca tcctggaaag tcacatcgat gcgaattgat aataattcaa ccgcaacagc
    13321 agtattgtat aaataaggat gtttacaatg gagaaatacc tgcacatgtt aagtcgggga
    13381 gataaaattg gcctgacatt gattcgtctg agtattgcga ttgtttttat gtggattggg
    13441 ttattaaagt ttgtccctta cgaggcagac agcattacac cattcgtcgc aaacagtcca
    13501 ctaatgtcgt tcttttatga acacccggaa gactataaac agtatctgac tcacgaaggc
    13561 gaatacaaac cagaagcaag ggcatggcaa tcggccaata atacctatgg tttttccaac
    13621 ggtcttggcg tcgtggaggt gattattgct ctgctggttt tggctaatcc tgtcaatcgc
    13681 tggttaggtt tattgggagg gctgatggca tttaccacac cgttggtaac actctcattt
    13741 ttaatcacca ccccggaggc atgggtaccc gcattgggtg acgctcatca tggtttccct
    13801 tatttatccg gtgctggtcg cctggtattg aaagatactc tgatgctggc aggtgctgtc
    13861 atgataatgg cagattcggc gcgggaaatt cttaaacaac gcagtaatga atccagttca
    13921 acgttaaaaa ctgaatattg ataatccgaa cactgtctgt tgttcacttt ttcaggcgtc
    13981 aggccccctt tctgatgatg aagcctgacg ccatattacc ggagatatga cagagtgaag
    14041 atcaatatca ctcccctgtt aaaagattct tgtcgtctca ggtaaaatat atgattaata
    14101 tcaatcatga attttttaca gcaagacaac cacgaacaaa ttcaggatta aaatgtttta
    14161 ccgactcccc aacataaccc aaatcaagcg aactaatttg tgccatttct ttctctgtca
    14221 gtgagaaatc ccagatagca aaattttctt cgatacgctg ttgatgtgtc gatttcggaa
    14281 taacgaccac tccccgttga atattccaac gcagaatgac ttgagaaatc gatttttgat
    14341 gggcatctgc aatactctga agcagattat tttcaaaggg tttatgtcgt ccaccaccta
    14401 acggagccca tgcttcaggc tgcacgttat aatacttcat ggttgccagc gcctctggtt
    14461 gggcaaaata gggatgcaac tcgacctggt tgaccatcgg tttaactctt accgtttcac
    14521 aaaagttcgc cagaacatga ggatagaaat tggaaacccc aattgctttt aatttgccct
    14581 cttcataggc atcttcgagt gcacgccacg cactgaaata atcgcgcata gcctggtgca
    14641 ataaatagag gtcgaagtat tctagtcccg atttttttaa tgatgcttca atacctgctg
    14701 ctgccgtatc ctgattcaac atatcctgca cccacaattt agacgttata aataactcat
    14761 ctcgggtaca taaaccttca gatatagctt cacgaacggc ttcaccgacg gcatcttcat
    14821 taccgtatac cgctgcagtg tcgatgagtc gataacccgt gcggatagcg ttcaacactg
    14881 actgtttgca cacgttttta tcggtaacct gaaaaacacc aaatcccatc atcggcattt
    14941 tcagattgtt acttaatacg gaaaattcca ctgtcagtcc ctccattaga catgaacaat
    15001 gagtctacgt taaaacgtaa cctcaaatta gtatgtggat tttgatatca cttatgcaaa
    15061 aaattcatta ataatgtagg actgaaacct ctctattttc ggggacaacg aagcagacgc
    15121 taccagtgct tttgccttcg gacttgctgt ttttgataca cttagggccc agggcataac
    15181 gaaaatgtgc gatatgacga agaatcaatg gagaatgtga tgatcaggca gaagattcta
    15241 cagcagctcc tggagtggat tgagtgcaat cttgagcacc ctatttcaat cgaagatatc
    15301 gcacagaaat ctggctacag cagacgcaac atccagcttc tgttccgaaa tttcatgcat
    15361 gtgcctttgg gagaatacat tcgcaaacga aggctttgcc gtgccgctat tcttgtccgg
    15421 ctcaccgcga aatctatgct tgatattgca ctctctttgc attttgattc acagcagtca
    15481 ttcagccgcg aatttaaaaa gttattcggc tgctctcccc gtgaataccg ccaccgtgat
    15541 tattgggatc tcgcaaatat cttcccttct tttttaatac gtcaacagca aaagacggag
    15601 tgtagattag tcaattttcc tgagacacct atttttggca actcatttaa atatgacatt
    15661 gaagtgtcga ataaatcacc ggatgaagaa gtcaaactgc gacgtcatca tttagccaga
    15721 tgtatgaata attttaagac ggatatctat ttcgtctcca cgtttgaacc gtcaacaaaa
    15781 tcggtcgatt tgctcacagt tgaaactttt gccggtacgg tatgtaaaca aaccgattct
    15841 gagatggcga aagagtggac aataaaccga ggactgtatg catctttccg cgatgaaggt
    15901 gagtgggagc gctatcctga atggtcgcga aacctctatc tgatggaatt acctgccagg
    15961 gggttagcca gagtgaacgg cagtgatatt gagcgatttt attacaatga agatttcgta
    16021 gaaaaggata gcaatgatgt tgtttgcgaa atttttatcc ccgttcgtcc ggtttagctg
    16081 tcgctatatc taaatgaact tataacgaga ctactgtcag gcaattcaat aaataatggc
    16141 gttgaatatg tcaacgccat tattgtttat tagaatgcta ttttcatatt cttaaaaatt
    16201 atttcatcgc ctcctcgccc tttgccgtat cggcatccac cgcggtaatg gtcaccgtca
    16261 ctggcttcat ttcgtacccg ccataggtca gggcgttaaa tgtgaatgta tagacgcctg
    16321 gcttgctggt actaaatgta cgtatgattt tcccgctcac gaaatggtca tttttcgacg
    16381 gcaggaactg ataatccttc tccgtcaccc cttccggcgt ctgaatgtcg acccacatat
    16441 tcccgccaac ggggttattt gccgacatca gggtcaccgc cagcttcgca gtcgatttcc
    16501 catccgccac cagagattgc atatcagatg atgcggtgag taccgcgttt ttatcatccg
    16561 caacgaattt agattcggca gttttcgttg atacctgacc gttggtctga ctcacttgcg
    16621 ccttgagtgt gtatgtcgcc gcaacaatag tggtagcggt gaaaatagcc tgcccttgct
    16681 cattcgttgt cgcagtgcca ttcggttcca ggtttaacga agccgggctg gcagtcagcg
    16741 tcacttcgct atctttcacc aaattgttat tggcatcagt caccgtaact ttatacgttt
    16801 gtttggcttt gccgtccgcc agcgcatagt tgccaatcac ctcaatattg gagatagttg
    16861 ccgtagtctt atccgccaca aacgttacag actgcgcatt cgaaggctgg tcatttcctg
    16921 ccgacgccgt gatctcatag gttccggcag tcactgaaac cacctgcaac tctgctttcc
    16981 cctcatcgtt ggctttcacc cagacattat cgcctgtaag cggcttgaca ccccggggca
    17041 gattaaaggt caccaggctc cctggaacag ggttaccatt tttatcctca acggtcgccg
    17101 tcagcgtaaa ggcgtctttg ccgtttgcca ctttttgaac ggcggtgtct acctgcaggt
    17161 ttgcctgacc ggtgctggca tccgcgttaa atttcaccgt gaccgtcttc tgagcaccat
    17221 tcaccgaagc ggaaatattg tgttctcccg caaccgttga cataagctca atgtccactt
    17281 tccctgccgc attggttgtt actgtattcc ccgtcttgct ggtcacaccc tgcggtaaag
    17341 tcagcgtaac ttcttgcccc tgcaacgggt taccatagct gtccacgacg gtcagggtta
    17401 tctggttagc agactgtcca ttagccagtt ggttattaac cttcactgtc atatcaccaa
    17461 tctcagcctt agatgcgtca cctgcaacgt tcagcaccag tggctgagca acggcatttt
    17521 ggccgttcac tcgcggcatc acagacaact gacccgcggc agatcccagc gttaaggtcg
    17581 acacgtagac cccattacct ttctctgtcc agcttcctgc tgaaggacgc tcactccccg
    17641 tgctggcggc accgctaaac actggtgcat ctggcttcag cccggtgacc gggttcccgt
    17701 acgcatcctt cacggtgaag gtgagttccg tcgtcgtttt gacggtcgga gccttattgt
    17761 ccgcaacaag cgtagagttc gctgatgaca tctctccggc gatgaccgtc aactgcgcgg
    17821 cttcggtggc tgctggctgg ccgttgaaga gcggcatgac acgaagctcg cccgtctttc
    17881 cgcctgtagt caacgtagca acataggaac cgtcaccttt ttcggtccaa ctggaaacgg
    17941 tcgccccttc agaggcggtc cccgtcaaac ttgccgacaa cgaaagacca ctgatagcgt
    18001 tgccatgcgc atctttcgcc accagcgtca cggttgtact ttcgccggct tttacgtgat
    18061 cttctgcgac agagactttc gactggtttg aagataacgc atcagccacc acggttactt
    18121 ttgccgcatt tgctgccgca tcctgtccat ttagcttcgg cataacttct aattcacccg
    18181 ccgtagagcc gagagtaatc tgcgcagtcc acgtcccatc accattattt gtccagcccg
    18241 atgccgtaga acctacagca gcggcacccg ctaatgacgg cgcttccggc gtgaggctgg
    18301 tcacagggtt gtcataggca tcttttaccg tccagattgc cgtaactgta cccccaacca
    18361 ccggtttatc aggattcagg gtgatggacg aatgtgctgc atcaagcggc ccggcaacaa
    18421 acttcagcgt ttgttgtaat gtgcccagct gatactgctc gctgaaagca cgcacaacaa
    18481 cgtttccggc acgggtcgaa gaaaccgtag cgctgtaaac gcctggtttt atctccgaaa
    18541 ttgtaccaat ggtgacacca ttagtgtctt gcggaacaaa tcgcaagcgg ctggcctctc
    18601 ccgtcaccgg gttaccatca gtatccaccg cagtcagcgt gtaggcttgc tgaccatcag
    18661 caaccacgtc acctgacggc tcgttagcgc tcagggtgga gtttgccaca tccatcatcg
    18721 tggcccgcag ttctgcagtg acggttttgc tcatgccatc aacactaaca gtaatcgttg
    18781 cctcacctga ctgcgttccg gtagtaaaga cagactgata cacccctgct tcagtttcgg
    18841 tgaactcacc cagtgttggc tttgcctgtg atttagtggc cttcagggta cgagtcacaa
    18901 tatttccagc cggtttgaaa gttagttcag tcttgatctg atctttcatg cccgtgactg
    18961 gctggccctc ggcgtcgcgc agagacagca ccagcggtct ttgctcatta ccgttagcaa
    19021 gcatttgaat acggctctga ccgtcaagcg ttaacgccgt gcgatcggca ctcatacctg
    19081 ctccggtaat gaccacctct gtctgcacgc gttttgaggc attgcctttg ttatcgtagg
    19141 caaccgcaga aatcgcataa taattgtctt tgcctggacg ataagccggg agcgttactt
    19201 gccactgact accctgaccg gtaattttgc caccttcagc cagtaatgac ggcgcttccc
    19261 actgcacatt tttcagtccg tgagttgctt tgctgaccac aagccccagg gaaagtgtct
    19321 gaccgccctt accttcaata cgctcaggca gagcaatacg gatcacttca gatttgcggt
    19381 actcaagaac gatgttgtta ttacgctcaa ccaggtcata gcggctgcct gccagcattc
    19441 gacgctcgcg aatgctgtct gtatcgagtt gtttctccag aggttcgcca atccgataat
    19501 taacttccag gccaaagcga gtgtcattct caccgctctt gccctgctta tgcccggcgc
    19561 tcagtgtcag aagaggcact ggcgtatagg tcacctcggc agaaatagca tgcgggtctt
    19621 tctggcgctt atctttacca aacagcccga cttcatcgcc ataatactgt tcatacatca
    19681 ggcttgcgcc aagctgcggc caggcaggta aatagccctc tgcgcggata tcccaaccat
    19741 tcgccgggcg ttcctgataa tcctcaatat ccggcgattt tttccagcca gaagcccgaa
    19801 tataaccatt ggcgctcagt ttcagataat cgcgccagta ttccgcacca acaccaatgc
    19861 gggtatgact acgggataaa tcatggtcga taaaggtgtt cacccccgcc atccagtcat
    19921 ttcctgaaaa atgacgccag ccaaaaccaa tatttgactg agtacgatcg tcggtacgat
    19981 gtattgctcc ctgagtgaac aacatatttg ttggcgtatc ataaatcgga taaagcattt
    20041 ccagcgaaga atccttcagc gagaaatctt tatcgacatt cagtttgacg cgcgcagtac
    20101 catatttccc gagccactcc tgtatttcct ggttagcttt agctgtggcc attccggtaa
    20161 taaagttacg tgtcgcatcg ctatctggct gactgcttaa aaatgtcccg gcatttgcgg
    20221 caaacgacgc gacatttttc tccacgttat tatcagcagt taccgtagta tttcccatgc
    20281 tcaaccgtgg ctgaaccgca tgctgcgcac gtgccgccat tactgggtaa aggtgacagc
    20341 gagtggaaaa agaacctgaa cagagatatt tgcccacgcc acgcagcggg ccagaactga
    20401 ataacgaaat cgtggttgtt tatgacctgt tttataacgc gacattgaga taatttcctg
    20461 atgaacgaat taacgaacat aggcagacac aaataatggc gtctgcttgt cagaaaaacc
    20521 ggggatttcc cgatttcctt ttccgcagaa aaggaaatac ctttatgtaa gaaagtgcac
    20581 cgccacctaa tatatccagt gtctgtagcc actcatcagg tggttgggcg cgggattata
    20641 tttttattta tgggtgaaaa tttagctttt tgtgcaagtc attaaataga cacctaagtt
    20701 atatttttta ctctgcggac agtgaataat tcattattca gagtgaataa taatggcctg
    20761 cctttaatta acaa
//
LOCUS       DAZFQD010000089        20733 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.089, whole genome shotgun sequence.
ACCESSION   DAZFQD010000089 DAZFQD010000000
VERSION     DAZFQD010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20733
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.089"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..1044)
                     /locus_tag="ACHKTW_003270"
     CDS             complement(<1..1044)
                     /locus_tag="ACHKTW_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6531 domain-containing protein"
                     /protein_id="HFO6464237.1"
                     /translation="MSGKPAARQGDMTQYGGPIVQGSAGVRIGAPTGVACSVCPGGMT
                     SGNPVNPLLGAKVQPGETDLALPGPLPFILSRTYSSYRTKTPAPVGVFGPGWKAPSDI
                     RLQIRDDALVLNDNGGRSIHFEPLLPGEAVYSRSESMWLVRGGKAVQPDGHTLARLWA
                     SLPPDIRLSPHLYLATNSAQGPWWILGWSERVPGAEDVLPAPLPPYRVLTGMADRFGR
                     TLAYRREAAGDLAGEITGVTDGAGREFRLVLTTQAQRAEEARKPHTASLSSPDSPRPL
                     SAPSFPDTLPGTEYGADSGIRLSAVWLMHDPEYPENLPAATLVCYDWTPRGELAAVYD
                     RSGTQMRHFTYDDK"
     gene            complement(1474..3888)
                     /gene="ybbP"
                     /locus_tag="ACHKTW_003271"
     CDS             complement(1474..3888)
                     /gene="ybbP"
                     /locus_tag="ACHKTW_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease subunit YbbP"
                     /protein_id="HFO6464238.1"
                     /translation="MIARWFWREWRSPSLLIVWLALSLAVACVLALGNISDRMEKGLS
                     QQSREFMAGDRALRSSREVPQAWLEEAQKRGLKVGKQLTFATMTFAGDTPQLANVKAV
                     DDIYPMYGDLQTNPPGLKPQAGSVLLAPRLMALLNLKTGDTIDVGDATLRIAGEVIQE
                     PDSGFNPFQMAPRLMMNLADVDKTGAVQPGSRVTWRYKFGGNENQLDGYEKWLLPQLK
                     PEQRWYGLEQDEGALGRSMERSQQFLLLSALLTLLLAVAAVAVAMNHYCRSRYDLVAI
                     LKTLGAGRAQLRKQIVGQWLMVLALSAVTGGAIGLLFENVLMVLLKPVLPAALPPASL
                     WPWLWALGTMTVISLLVGLRPYRLLLATQPLRVLRNDVVANVWPLKFYLPIVSVVVVL
                     LLAGLMGGSMLLWAVLAGAVVLALLCGVLGWMLLNVLRRMTLKSLPLRLAVSRLLRQP
                     WSTLSQLSAFSLSFMLLALLLVLRGDLLDRWQQQLPPESPNYFLINIATEQVEPLKAF
                     LAEHQIVPESFYPVVRARLTAINDKPTEGNEDEALNRELNLTWQNTRPDHNPIVAGNW
                     PPKADEVSMEEGLAKRLNVALGDTVTFMGDTQEFRAKVTSLRKVDWESLRPNFYFIFP
                     EGALDGQPQSWLTSFRWENGNGMLTQLNRQFPTISLLDIGAILKQVGQVLEQVSRALE
                     VMVVLVTACGMLLLLAQVQVGMRQRHQELVVWRTLGAGKKLLRTTLWCEFAMLGFVSG
                     LVAAIGAETALAVLQAKVFDFPWEPDWRLWIVLPCSGALLLSLFGGWLGARLVKGKAL
                     FRQFAG"
     gene            complement(3885..4571)
                     /gene="ybbA"
                     /locus_tag="ACHKTW_003272"
     CDS             complement(3885..4571)
                     /gene="ybbA"
                     /locus_tag="ACHKTW_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter ATP-binding protein
                     YbbA"
                     /protein_id="HFO6464239.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            4542..5165
                     /gene="tesA"
                     /locus_tag="ACHKTW_003273"
     CDS             4542..5165
                     /gene="tesA"
                     /locus_tag="ACHKTW_003273"
                     /EC_number="3.1.1.5"
                     /EC_number="3.1.2.14"
                     /EC_number="3.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016298 - lipase activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
                     /protein_id="HFO6464240.1"
                     /translation="MNFNNVFRWHLPFLFLVLLTFRAAAADTLLILGDSLSAGYRMSA
                     SAAWPALLNDKWQSKTSVVNASISGDTSQQGLARLPALLKQHQPRWVLVELGGNDGLR
                     GFQPQQTEQTLRQILQDVKAANAEPLLMQIRLPANYGRRYNEAFSAIYPKLAKEFDVP
                     LLPFFMEEVYLKPQWMQDDGIHPNRDAQPFIADWMAKQLQPLVNHDS"
     gene            5155..5964
                     /gene="ybbO"
                     /locus_tag="ACHKTW_003274"
     CDS             5155..5964
                     /gene="ybbO"
                     /locus_tag="ACHKTW_003274"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HFO6464241.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPDDVERMNNMGFTGVLIDLDSPESVDRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLKRLLPGRVMDKILQG"
     gene            6025..6879
                     /gene="cnoX"
                     /locus_tag="ACHKTW_003275"
     CDS             6025..6879
                     /gene="cnoX"
                     /locus_tag="ACHKTW_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HFO6464242.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQESNYTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADTPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(6942..7721)
                     /gene="fetB"
                     /locus_tag="ACHKTW_003276"
     CDS             complement(6942..7721)
                     /gene="fetB"
                     /locus_tag="ACHKTW_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HFO6464243.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGSIEFIPMQVIPIAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(7708..8385)
                     /gene="fetA"
                     /locus_tag="ACHKTW_003277"
     CDS             complement(7708..8385)
                     /gene="fetA"
                     /locus_tag="ACHKTW_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HFO6464244.1"
                     /translation="MQENSPLLQLQNVGYLAGDAKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRNQQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            8531..9448
                     /gene="qmcA"
                     /locus_tag="ACHKTW_003278"
     CDS             8531..9448
                     /gene="qmcA"
                     /locus_tag="ACHKTW_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFO6464245.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            9445..9903
                     /gene="ybbJ"
                     /locus_tag="ACHKTW_003279"
     CDS             9445..9903
                     /gene="ybbJ"
                     /locus_tag="ACHKTW_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFO6464246.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVSAVITGL
                     VVWLVPLGWEWQGVMFAILTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLHIRAVSS"
     gene            complement(9904..10311)
                     /gene="cueR"
                     /locus_tag="ACHKTW_003280"
     CDS             complement(9904..10311)
                     /gene="cueR"
                     /locus_tag="ACHKTW_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HFO6464247.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     DQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(10308..10405)
                     /locus_tag="ACHKTW_003281"
                     /pseudo
     CDS             complement(10308..10405)
                     /locus_tag="ACHKTW_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308571.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(10436..11728)
                     /gene="ybaT"
                     /locus_tag="ACHKTW_003282"
     CDS             complement(10436..11728)
                     /gene="ybaT"
                     /locus_tag="ACHKTW_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFO6464248.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWVAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASAINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLGSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGSLLLSLIVAMVMKRNKTV"
     gene            complement(11731..12663)
                     /gene="glsA"
                     /locus_tag="ACHKTW_003283"
     CDS             complement(11731..12663)
                     /gene="glsA"
                     /locus_tag="ACHKTW_003283"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HFO6464249.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPSQLAAVA
                     IVTCDGNIYRAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENIEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            12925..15429
                     /gene="copA"
                     /locus_tag="ACHKTW_003284"
     CDS             12925..15429
                     /gene="copA"
                     /locus_tag="ACHKTW_003284"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HFO6464250.1"
                     /translation="MSQTIDLTLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFAGGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARLVTDEGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGESVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNEQQVGTKAIEAEITA
                     QASQGATPVLLAIDGKAVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            complement(15543..15884)
                     /gene="ybaQ"
                     /locus_tag="ACHKTW_003285"
     CDS             complement(15543..15884)
                     /gene="ybaQ"
                     /locus_tag="ACHKTW_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFO6464251.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFRLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            16022..16816
                     /gene="ybaP"
                     /locus_tag="ACHKTW_003286"
     CDS             16022..16816
                     /gene="ybaP"
                     /locus_tag="ACHKTW_003286"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HFO6464252.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDITLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPACEALEERISEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQIAMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNEITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            17020..17499
                     /gene="ybaK"
                     /locus_tag="ACHKTW_003287"
     CDS             17020..17499
                     /gene="ybaK"
                     /locus_tag="ACHKTW_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HFO6464253.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMVAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(17536..19188)
                     /gene="ushA"
                     /locus_tag="ACHKTW_003288"
     CDS             complement(17536..19188)
                     /gene="ushA"
                     /locus_tag="ACHKTW_003288"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HFO6464254.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNPEYFTDVEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGETNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            19406..20626
                     /gene="fsr"
                     /locus_tag="ACHKTW_003289"
     CDS             19406..20626
                     /gene="fsr"
                     /locus_tag="ACHKTW_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HFO6464255.1"
                     /translation="MAMSEQPQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYASLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLL
                     GMLTIFLPDNRHKD"
BASE COUNT         4851 a         5679 c         5393 g         4810 t
ORIGIN      
        1 tttatcatcg taagtaaagt ggcgcatctg cgtaccgctg cggtcataca ccgccgccag
       61 ctcaccgcgt ggcgtccagt cgtagcacac cagtgtcgcg gcaggtaaat tttccgggta
      121 ttccgggtcg tgcatcagcc acaccgccga aagacggata ccgctgtcag cgccgtattc
      181 cgtaccgggc agtgtgtcgg ggaaggaagg agctgagaga gggcggggac tgtcagggga
      241 agataacgaa gcggtgtgcg gtttacgggc ctcttccgcc cgctgtgcct gtgtggtcag
      301 taccagacgg aactcccgcc cggcaccgtc cgtcacgccg gtgatttccc cggccaggtc
      361 accggcggcc tcacgccggt acgccagcgt ccgtccgaag cggtccgcca tcccggtaag
      421 cacccggtac ggcggcagcg gcgctggcag tacgtcctca gcacccggca cccgctcaga
      481 ccaccccagt atccaccacg gcccctgtgc gctgttggtc gccaggtaaa gatgcgggct
      541 taaccggata tccggcggca gcgacgccca cagccgtgcc agcgtgtggc cgtccggctg
      601 cactgcctta ccgccacgca ccagccacat tgactcactg cggctgtaca ccgcctcccc
      661 cggcagcagc ggctcaaagt gaatgctccg cccgccgttg tcgttgagta ccagtgcatc
      721 atcgcgtatc tgtaagcgga tatcagaagg cgctttccag ccggggccga aaacgcccac
      781 cggtgcaggc gtcttcgtcc ggtagctgct gtaggtgcgg gagagaatga acggcagcgg
      841 gccgggcagc gcaaggtccg tctcgccggg ctgtactttt gcaccgagca gcgggttcac
      901 cgggttgccc gaggtcatcc cgcccggaca caccgagcac gccacgccag ttggcgcgcc
      961 aattcttaca cctgccgaac cctggacaat gggaccgcca tactgagtca tatctccctg
     1021 acgcgccgct ggttttccgc tcatataaag tgccctttta gttattgttc atatccctgt
     1081 gttagcaaca aaaaaaataa aatgacacac aagaccagct aatgtattta tatatttttc
     1141 acataaactt atttgttctc aatcaacaac aactgttatt acaggtcact ttcaatattt
     1201 tattaaacta aaatttttag cacagatgaa ttcatcatca acaaattcac tatatggata
     1261 atatccattc aacgcatatg atttatgatt ttattagtct gagttatttt tgtagggcta
     1321 tatttaaaat tattaaactc caccgattca gattaatatc agcaatagaa agataggaaa
     1381 tcaccaattc aataacatca tcaatgaaac atattgtaga attaatttaa acaaggcgtt
     1441 ccattcaacc agaacgcccc ttatttactc tcatcacccc gcaaactgcc tgaacagcgc
     1501 cttaccctta accagtcgcg cacccagcca gccgccgaaa agcgacagca gcagcgcacc
     1561 gctgcacggc agaacaatcc acaatcgcca gtctggctcc cacgggaaat caaacacttt
     1621 cgcctgcaac actgccagtg ccgtttccgc accaattgcg gccaccaggc cggaaacaaa
     1681 cccaagcatg gcgaactcac accacaatgt cgtgcgcagc agttttttcc ccgcaccgag
     1741 tgtgcgccat accaccagct cctgatgacg ctggcgcatt cccacctgca cctgtgccag
     1801 caacagcaac ataccgcagg cggtgaccag caccaccatc acctccagcg cccgacttac
     1861 ctgctccagc acctgaccga cctgttttaa aatcgcgcca atatctaaca ggctaatggt
     1921 cgggaactgg cggttgagtt gcgtcaacat gccgttgcca ttctcccagc ggaaactggt
     1981 aagccagctc tgcggttgcc cgtctaatgc cccttccggg aaaataaaat agaaattagg
     2041 ccgcagactt tcccagtcca ctttgcgcag gctggtcact ttagcgcgga actcctgggt
     2101 atcgcccata aaagtcacgg tatcaccgag ggcaacgttt aagcgtttcg ccagcccctc
     2161 ttccatcgac acttcatcgg cttttggcgg ccagttaccg gcgacaatcg gattatgatc
     2221 gggccgcgta ttttgccagg taagattgag ttcgcgatta agcgcctcat cttcattacc
     2281 ttctgtcggc ttatcgttaa tcgccgtcag ccgcgcccgc actaccggat aaaatgattc
     2341 cgggactatc tgatgttccg cgaggaacgc ttttagcggt tctacctgtt ctgtggcaat
     2401 gttaattaaa aagtagttcg ggctttctgg aggtagctgc tgctgccagc ggtcaagcag
     2461 atcgccacgc aataccagca acagcgccag cagcataaag gagagcgaaa atgccgaaag
     2521 ctggcttaat gttgaccacg gctgacgtaa caggcggcta accgccaggc gcagaggtag
     2581 cgatttcagc gtcatgcggc gaagtacatt cagcagcatc cagcccagca caccgcacag
     2641 caaagccagt actaccgcgc ccgccagcac cgcccaaagc agcatgctgc cacccattaa
     2701 tccggcgagc agcagcacaa ccaccacact gacaatcggc agataaaact tcagcggcca
     2761 gacgttcgct accacatcat tacgtaatac gcgtaaaggc tgcgttgcca gcaacaggcg
     2821 gtacggtcgt agccccacca gcagcgagat gaccgtcatg gtacccagcg cccacagcca
     2881 cggccagagg ctggctggcg gtagtgcagc aggcagaacg ggcttgagca gcaccatcaa
     2941 cacgttttcg aacaacaggc ctatcgcccc accggtaacg gctgaaagcg ccagcaccat
     3001 caaccactga ccgacgattt gcttacgcag ttgcgctcgc cctgccccca gcgttttgag
     3061 gatcgccacc agatcgtagc gactgcgaca gtaatgattc atcgctaccg ccaccgctgc
     3121 cactgccagc agcaaggtca gaagcgccga aagcagcagg aactgttgcg agcgttccat
     3181 cgatcgcccc agcgcgcctt cgtcctgttc cagaccgtac cagcgttgtt cgggtttaag
     3241 ctgaggtaac aaccatttct catagccgtc gagctggttc tcgttgccgc cgaatttata
     3301 acgccaggtg acccgactcc ccggctgcac ggctccggtt ttatcgacat ccgccagatt
     3361 catcatcaga cgcggagcca tctggaaggg gttaaaaccg gaatccggtt cctgaatcac
     3421 ttctccggca atccgcaagg tggcatcgcc cacgtcaatg gtgtcgcccg ttttcaggtt
     3481 aagcagcgcc atcaggcgtg gggccagcaa tacgctgccc gcctgcggtt tcaggccagg
     3541 gggattagtt tgcagatcgc catacatcgg gtagatatca tccaccgctt tgacgttcgc
     3601 cagctgcggt gtgtcgcctg caaatgtcat ggtggcgaaa gtcagctgct tgccgacttt
     3661 caggccgcgc ttttgcgctt cctccagcca tgcttgcggc acttcgcgcg aactgcgcaa
     3721 cgcccgatcg cccgccataa actcccggct ttgttggctt aagcccttct ccatgcgatc
     3781 gctgatattg cccagcgcca gcacgcaggc caccgccagg cttagcgcca gccagacaat
     3841 taatagcgac ggcgaacgcc attcgcgcca gaaccaacgt gcaatcatgc ttcctcctgc
     3901 aactgcccgt tcaccagccg taagcagcgg tcgcagcgtg ccgccagttg caggtcgtgg
     3961 gtcaccataa tcaacgtggt gccatgttca cggttgaggg aaaacagcag gtcggcaatt
     4021 ttatcgcccg tctggcggtc aaggttgccg gtgggttcgt cggcaaacag cacatcaggt
     4081 cgaccattaa aggctcgtgc cagcgccact cgttgctgtt caccgccgga aagctgtgcc
     4141 ggaagatgat ccagacgttt acccagccct aactgttcga gcaacgcttt cgccccgtta
     4201 cgactttccg cgctactctc accgcgcagc agagccggaa gctcgacgtt ttccagcgcg
     4261 ttaagggtag gaattaacat aaatgactga aaaacaaagc cgacgtgctt cgcgcgcaac
     4321 tttgcccgcg cttcttcgtc catattatgt agcggttgtc ccaccagact cacttcgcca
     4381 ctgctgccgt catcaagccc ggcgaggatc gccagcaagg ttgacttacc cgatcccgac
     4441 tcgcccacca gtgcgatggt ctcgccacgt ttgacaacca gctcaactcc ggtgaggatg
     4501 gagagttcat gctccccctg accgacggac ttcttaagat gatgaacttc aacaatgttt
     4561 tccgctggca tttgcccttc ctgttcctgg tcctgttaac cttccgtgcc gccgcagcgg
     4621 acacgttatt gattctgggt gatagcctga gcgccgggta tcgaatgtct gccagcgcgg
     4681 cctggcctgc cttgttgaat gataagtggc agagtaaaac gtcggtagtt aatgccagca
     4741 tcagcggcga cacctcgcaa caaggactgg cgcgccttcc ggctctgctg aaacagcatc
     4801 agccgcgctg ggtgctggtt gaactgggcg gcaatgacgg tttgcgtggt tttcagccac
     4861 agcaaaccga gcaaacgctg cgccagattt tgcaggatgt caaagccgcc aacgctgaac
     4921 cattgttaat gcaaatacgt ctgcctgcaa actatggtcg ccgttataat gaagccttta
     4981 gcgccattta ccccaaactc gccaaagagt ttgatgttcc gctgctgccc ttttttatgg
     5041 aagaggtcta cctcaagcca caatggatgc aggatgacgg tattcatccc aaccgcgacg
     5101 cccagccgtt tattgccgac tggatggcga agcagttgca gcctttagta aatcatgact
     5161 cataaagcaa cggagatcct gacaggtaaa gttatgcaaa aatcggtctt aattaccgga
     5221 tgttccagtg gaattggcct ggaaagcgcg ctcgaattaa aacgccaggg ttttcatgtg
     5281 ctggcaggtt gccgaaaacc ggatgatgtt gagcgtatga acaacatggg atttaccggc
     5341 gtgttgatcg atctggattc accagaaagt gttgatcgcg cagcagacga ggtgatcgcc
     5401 ctgaccgata attgtctgta tgggatcttt aacaatgccg gattcggcat gtatggcccc
     5461 ctttccacca tcagccgtgc gcagatggaa cagcagtttt ccgccaactt tttcggcgca
     5521 caccagctca ccatgcgcct gttacccgcg atgttaccgc acggtgaagg gcgtattgtg
     5581 atgacatcat cggtgatggg attaatctcc acgccgggtc gtggcgctta cgcggccagt
     5641 aaatatgcgc tggaggcgtg gtcagacgcg ctgcgcatgg agctacgcca cagcggaatt
     5701 aaagtcagcc tgatcgaacc cggtcccatt cgtactcgct tcaccgacaa cgtcaaccag
     5761 acgcaaagtg ataaaccagt cgaaaatccc ggcatcgccg cccgctttac gttgggaccg
     5821 gaagcggtgg tggacaaagt acgccatgct tttattagcg agaagccgaa gatgcgctat
     5881 ccggtaacgc tggtgacctg ggcagtaatg gtgcttaagc gcctgctgcc ggggcgcgtg
     5941 atggacaaaa tattgcaggg gtgagttgaa gcgcgcgctt aagcccccat gtcaaagaaa
     6001 atatcgacaa cagagagcga ctccatgtcc gtagaaaata ttgtcaacat taacgaatct
     6061 aacctgcaac aggttcttga acagtcgatg accactccgg tgctgttcta tttttggtct
     6121 gaacgtagcc agcactgttt gcagttaacc ccgatcctgg aaagcctcgc ggcgcagtac
     6181 aacgggcaat ttattctggc gaagctggac tgcgacgcgg agcagatgat tgccgcccag
     6241 tttggtctgc gcgcgattcc gaccgtgtat ctgttccaga acgggcaacc ggtagatggc
     6301 ttccaggggc cgcaaccgga agaggcgatc cgcgccctgc tggataaagt gctgccgcgc
     6361 gaagaagagc tgaaagcgca gcaggcgatg caactgatgc aggaaagcaa ttacaccgat
     6421 gccctgccat tgctgaaaga cgcctggcag ttgtcgaatc agaacgggga gatcggcctg
     6481 ctgctggcag aaacgctgat tgcgctgaac cgttctgaag atgcggaagc ggtgctgaaa
     6541 accattccgt tgcaggatca ggacacccgc taccaggggc tggtggcgca aatcgaactg
     6601 ctgaagcagg cggctgatac tccggaaatt caacagttgc aacagcaggt ggcggagaat
     6661 ccagaagatg ccgcactggc gacgcaactg gcgctgcaac tgcatcaggt tgggcgcaat
     6721 gaagaggcgc tggagttgct gttcgggcat ctgcgtaaag atctcaccgc cgcagacggt
     6781 cagacgcgta aaacgttcca ggagatcctc gctgcgctgg gtacgggtga tgcactggcg
     6841 tcgaagtatc gccgccagct gtatgcattg ttgtattgat attccgttga tcggcacatg
     6901 aaccacaacg ccggatgcga tgctgcgcat ccggcatcgc atcatttctt cttcaattgc
     6961 gtcaccacca actggtggcg cgaattataa aacttacgat aggttaaata gcaggcaata
     7021 atggtcgaca agctggcggt tgagagcagc ataaaggtca ccataatctg atatttaatc
     7081 gccttcaccg gatcaatccc ggcaaatatc agcccggaca tcattcctgg taaactcact
     7141 aagccaaccg tttttgctga atcgaccgtc ggaattaaag ccgcgcgaat actgtcgcga
     7201 atcaatatcg ctgaagcctg cttcggcgtc gcaccaagac tcagtttctc ctgaatctgt
     7261 tgctgttcgc taatgacccg ttgccctaaa ttgttgtaac acaaccccac cgctaccatg
     7321 gcgttaccgg caatcatccc ggcgataggg atcacctgca tcgggataaa ttcaatcgac
     7381 cctgagagaa tcagcaccgc cagggtaatt cccgccccga ccgtaatggc aataaacgat
     7441 gagataaaag ctttagcaat atatttactg cgtttttgcg cgttccacgc cgcattaaag
     7501 cagataaata acaccatcaa taatgtcagg ctggcatcat ccacgctgaa aatatacttc
     7561 agcacatagc cgacaataat cagctgaatt atcgctcgcc cgacgctcca gagaatatct
     7621 ttctccagcg ccagtttttc tttatggcta attaagattg ccaccaccac cagcattaat
     7681 gccagtgcta atgattcgtt agtaatatta tgcgagttca tagcgtgctt cctgcatttc
     7741 tccggcatgc ggttgcagtg taatcacttt atccgcatga ttaatttcgt ctttatcgtg
     7801 tgtcacccac agcacggcaa tattttgctc gcgcacataa cgatggatca tctcattgac
     7861 gttatgttta ttactttcat ccagcgcact ggttatttca tccagcaata aaaccttcgg
     7921 cataaattgc aggttacgaa tcaatgagat gcgttgtttt tcaccaccag atagctcggc
     7981 gatattcttc gtcaaaatgc tgtccggcaa agcgaagcgt tcgagaaaat cgagaaaaat
     8041 ggccgggtca ggctgctggt tacggatctg ccagggaaag atcagattat cgtataccgt
     8101 atcgccaaac agcgtcggcg tctgggcgca gtaagagact tgctggcggt agatttctgg
     8161 ctttagtgtg ctgacatcct caccttcaaa cagtaacgtt ccgctggttg ggctgatcaa
     8221 tgaagcaact atttttagca gcgtactttt gccacaaccg gaaggaccgg taattaactt
     8281 aaattcgcca gcacgcagcg aaaaattgat gttattaaga atcttcgcat cacccgccag
     8341 atatcctacg ttttgtagct gaagcaaagg actattttcc tgcatcactg ttcccttttt
     8401 ctgattttta ctaaaaacag tttatccttc gcaggaataa gggggaactc tctttcagta
     8461 atcaggtata atttgtttaa tcaagcagca attttgctga ttatggacgg tacaacagga
     8521 ggtttttccg atgcttatct ttatcccgat tctcattttt gtcgcgctgg tcattgtcgg
     8581 cgcgggcgtc aaaatcgtgc cgcagggcta tcaatggacg gtagaacgtt ttggtcgcta
     8641 taccaaaacg ttacagccgg ggctcagtct ggtggtgccg tttatggatc gcattggtcg
     8701 caagatcaat atgatggagc aagtgctcga tatcccttcc caggaagtta tctcgaaaga
     8761 taacgccaac gttaccatcg acgcagtctg ttttattcag gtgattgacg cgccgcgcgc
     8821 ggcttatgaa gtcagcaatc tggagctggc gatcatcaac ctgaccatga ctaacatccg
     8881 taccgtgttg ggttcaatgg aacttgacga aatgctctct cagcgcgaca gcatcaactc
     8941 acgcctgctg cgtattgtcg atgaagccac caacccgtgg gggattaaag tcacccgtat
     9001 tgaaattcgc gacgtgcgcc caccggcaga gcttatctct tcaatgaacg cgcagatgaa
     9061 agcggaacgt accaaacgcg cttacatcct tgaagcggaa gggatccgtc aggcggaaat
     9121 actcaaagcg gaaggtgaaa aacagtcgca aattctgaaa gcggaaggtg aacgtcagtc
     9181 ggcattctta caggccgaag cgcgtgaacg ttctgccgaa gcagaagccc gcgccaccaa
     9241 aatggtatct gaagccattg cctccggtga tattcaggcg gtgaactact tcgtagcgca
     9301 gaaatacacc gaagcgttac agcagatcgg ttcctccagt aacagcaaag tagtgatgat
     9361 gccattagag gccagcagcc tgatggggtc gattgccggg attgccgagc tggtgaaaga
     9421 cagcgccaac aaacggactc agccatgatg gagttaatgg tcgttcatcc acatattttc
     9481 tggctcagtc tcggcgggtt gctgctggca gccgagatgc tgggcggaaa tggttatttg
     9541 ctgtggagcg gcgtgtcagc ggtgattacc ggcctggtgg tctggctggt gccgctgggt
     9601 tgggagtggc aaggggtgat gtttgccatc ctgacgctgc tcgccgcctg gctgtggtgg
     9661 aaatggttgt cgcggcgggt gcgcgaacaa aagcacagcg atagtcattt aaaccagcgc
     9721 gggcagcagc tgattggccg acgttttgtg ctggaatctc cgctggtcaa cgggcgcggt
     9781 catatgcgcg tcggtgacag ttcatggcct gtcagcgcca gcgaggatct cggtgcaggt
     9841 acgcatgttg aagtcattgc gatagaaggg ataaccctac atatccgagc cgtctcgtct
     9901 taatcaccct gcccgatgat gacagcagcc ggagagattt tcgataatcg ggcagtcggc
     9961 gctgtcatcg ccagggcagg cattcgccag tgccagcagc tggtcgcgca tggattgcag
    10021 ctcctcaatg tgtcgttcga tctccgccac cttctccagc gtgcgccgtt tgacgtcggc
    10081 gctgtgccgc tgcgggtcgt taaacagatt caccagctcg ccgctctctt ccaggttaaa
    10141 gcccacctgc cgtgcctggc gcagtaaggt cagttcgttg agatgctgct gcgtgtaggt
    10201 gcgataaccg ttttcgctgc gcatcggcgg cgtcaccagc cccttctctt catagaagcg
    10261 aatggctttg ctggtcaggc cggtaatttt tgctacatcg ctgatgttca tcgttcgcgc
    10321 aacgcctcct gtgctttgtt aaagggtttc aacagatatt caagcacgtt tttttgccag
    10381 tgcgaatatc gaccgccacc acaataatac aggaagtact gcgcacagag agatgttata
    10441 cggttttatt gcgcttcatg accattgcca caataaggct gagtaagagt gagccaataa
    10501 tccatatcaa cgcacggcta ccctgggacc acaggctgta gataaagcca acgatcacca
    10561 acaacatcac caacgtacca acgataagaa tcggcaacga ggcgtgaata tcatgacgca
    10621 ggcggatcgc caccacaaac accgccaggt agcaaatcaa aaaggtggcg ctggcaacac
    10681 tggcgagtga gcctaaattt agcgccgccg tcatcagcat aatcaacacc acgacaatga
    10741 tgttgcccca ggtactctgc cgccacaggg atttattcat tagcttcggc agttcgcgtt
    10801 cgctgcccat gttgtccatg atgttaaaca cggcgaacag gttcgcgtta atggctgaag
    10861 ccgtcgccag taaagcgccg atgacgacga tcacataacc cacatgtccg agcagcggag
    10921 aagcagcctg cgctacggcg gtatcggcat atttttctaa ctctaatgcc gatacatcgc
    10981 taagcaaaac cagtgccagc gagatataaa gcaacgtggt aacgccaatc gccaccagaa
    11041 acgcccgcgg cataatgacc tgcggatctt tcactttatc cgccgcgttc gccatcatgc
    11101 caaagcccgc ataggcaagg aaggttatcc cgatacagga gaagaacgca ccggagctgg
    11161 ggggcgcaga gacggaaata tgcgccggtt gcagcgacca gacaccggca ataatcagca
    11221 ataacaggat catcatttta atgccgacga ggatcacttc cagccgccct accgcatggt
    11281 tgcttaagga gttgaaaagc gtcatcaccg caatgatccc caacgcgtag agcaaaataa
    11341 ggtgctcctc ctggctgcct tcatgcaaaa actgcacggc ataagcgcca aaagcacggg
    11401 cgaccatggc gatgctcacc gccagcgtca acaggtacag taacgagagc gccagcgaaa
    11461 agacgccgtt gcctaatccg cgacgaaaga agtcgataat gccgccattg ctgggatagc
    11521 tcgcccccag acgcgcatag gcataaccgg aaaacatcgc cacaataccg ccaaaagcaa
    11581 aggcgaccca ggtcgaggct tccattagca atgcagcctg ccccagcagc gcgaagatcc
    11641 ccgcccccac cattgcccca atgccgatgg aaacgacgtt ccatagaccg agaggtttgt
    11701 taccgttatt accttccgtg ttcatcatga tcagccctta aacacgttat agccgagttg
    11761 cttagcgacc gatgccacca ttttttgacc gcgaacactg ttgccttctt cgtccagcgg
    11821 tggtgagaac gcggcaattc ccatcacgcc agggacgacc gccagaatac cgccacctac
    11881 accgcttttg cccggtaaac caacgcgata cgcccagtca ccggagcgac catacagccc
    11941 ttccatcatc atttcggcca gaatgtacgg cacgttgtcg gcctgaagaa cgcgtttatg
    12001 cgtcagcgga ttcacaccac ccgccgccag cgtcgcgcca agcgttgcca gttcaacagt
    12061 attgatgagc gtggagcact gacgggtgta cacgtcacag gcttccattg catcacaata
    12121 gagatatccg gcggagtaca gcagccaggc aatggcccgg ttatggaagt tggttgtttg
    12181 ttccgactgg ttgacttcat cagagagcgc tacctgctca ccagccagtt gctgttggat
    12241 atgtaaaatt cgctgccagc gttgttcaat attttcagcg ttaatcaggc tggtggtggc
    12301 aatagcgcca gcatttacca gtggcgacag tggtttgccg ccatgcaact ctaaggcgat
    12361 aactgagtta aagggcaatc cggtcgggtc agcgccaatt ttgtcctgta ccgcctgcgg
    12421 gccgacatct tctaacgcaa gggctaacgt acagactttc gagatggatt ccagcgcaaa
    12481 gcggtaatca ctatcacccg cgcgatagat gttgccatcg caggtcacaa tagccactgc
    12541 tgccagttga ctaggtacat tcgccagaaa gggaatgtag tcggcatttt gtccgccgtt
    12601 aagtgagtga aattgggtgt aagcctgatc cactgcctgc tgtaatttgt ttgcatctaa
    12661 catcttttgt taactccttt ttatagatgc gggaggtaat tcctcacccc ggtgccgatt
    12721 ttcaggcatc ttgatttaac ttagcacccg caacttaact tcaggaaaac aaagagataa
    12781 atgtctaatc ctgatgcaaa ttgagccaat tttttaatct ttacggactt ttactcgcct
    12841 ggtttattaa tttcttgacc ttccccttgc tggaaggttt aacctttatc acagccagtc
    12901 aaaactgtct taaaggagtg ttttatgtca caaactatcg acctgaccct ggacggcctg
    12961 tcctgcggtc actgcgttaa acgcgtgaaa gaaagtctcg aacagcgccc ggatgtggaa
    13021 caagcggatg tgtctatcac tgaagcgcac gttaccggga ctgccagtgc agaacagctg
    13081 atcgaaacca tcaaacaagc gggttatgac gcatctgtaa gccacccaaa ggctaaaccg
    13141 ctggcggagt catcaatccc gtcggaagca ctgacagcgg tttctgaggc gcttccggca
    13201 gcgaccgccg atgacgatga cagccagcag ttgctgctga gcggcatgag ctgcgccagc
    13261 tgtgtcaccc gcgtacaaaa tgcgctgcaa agcgtaccgg gcgtcactca ggcacgggta
    13321 aacctggcgg agcgtactgc gctggtgatg ggcagtgcct ccccacaaga tttagtgcag
    13381 gcggtggaaa aagcgggcta cggcgcggaa gcgattgaag atgacgcgaa acgccgtgag
    13441 cgccagcagg aaaccgccgt tgctacgatg aagcgcttcc gctggcaggc aattgtcgca
    13501 ctggcggtgg gtatcccggt gatggtctgg gggatgattg gcgataacat gatggtcacc
    13561 gctgacaacc gcagcctgtg gttggttatc ggcctgataa ccctggcggt gatggttttc
    13621 gccggcggcc atttttaccg cagtgcatgg aaaagcctgc tgaacggtgc agcgacgatg
    13681 gatacgctgg tggcgctggg tactggcgtg gcgtggctct attcgatgag cgtcaacctg
    13741 tggccgcagt ggttcccgat ggaagcgcga catctttatt acgaagccag cgcgatgatt
    13801 atcggtctga tcaatctcgg ccatatgctg gaagcacgcg cacgtcagcg ttcttctaag
    13861 gcgctggaga aattactcga tttaacccca ccgacggcgc gcctggttac tgacgaaggt
    13921 gaaaaaagcg tgcctctggc ggaagtgcag ccaggtatgt tgctgcgcct gacgaccggc
    13981 gatcgcgtgc cggtagatgg cgagattacc cagggcgaag catggctgga tgaagcgatg
    14041 ctgacgggcg aaccaatccc gcagcaaaaa ggcgaaggcg aaagcgtcca tgccgggaca
    14101 gtggtacagg acggtagcgt gctgtttcgt gccagtgcgg ttggcagcca taccacgctg
    14161 tcacggatca ttcgcatggt gcgccaggcc cagagcagca agccagaaat cggtcagctg
    14221 gcggataaaa tctcagccgt atttgtacct gtggtggtgg tgattgcgct ggtcagtgcg
    14281 gcaatctggt acttctttgg tccggcaccg caaattgttt ataccctggt gattgccacc
    14341 acggtactga ttatcgcctg tccgtgtgcg ctggggctgg cgacgccgat gtcgattatt
    14401 tccggcgtcg ggcgggcggc tgagtttggc gtgctggtgc gggacgccga cgcgctgcaa
    14461 cgcgccagta cactcgatac cgtcgtgttc gataaaaccg ggacgctgac cgaagggaag
    14521 ccgcaggttg tcgcagtgaa aacgtttgct gatgttgatg aagcgcaggc attgcgtctg
    14581 gcggcggcac tggagcaagg ttccagccat ccgctggcac gggcgatcct cgataaagca
    14641 ggtgatatgc agctgccgca ggtcaacggt ttccgcacat tgcgcgggct gggcgtgagc
    14701 ggtgaagctg aaggtcatgc gttattgctg ggcaatcagg cgctgttaaa tgagcaacag
    14761 gttggtacca aagctatcga agcggagatt actgctcagg catcgcaagg agcaacgcct
    14821 gtgctgctgg cgattgacgg gaaagcagta gccctgctgg ctgtacgcga tccgttgcgt
    14881 agtgatagcg tggcggcgct gcaacgcctg cataaagcgg gatatcgtct ggtgatgttg
    14941 accggggata acccaaccac cgccaatgcg atcgccaaag aagctggaat tgatgaagtg
    15001 attgccggag tgctgccaga cggtaaagcc gaagcgatca aacgtctgca aagtgaagga
    15061 cgtcaggtgg caatggtggg cgacggcatt aacgacgcgc cagcgctggc ccaggcggat
    15121 gtcggcattg cgatgggtgg cggcagtgat gttgccattg aaaccgcggc gattaccctg
    15181 atgcgccata gcctgatggg cgttgcggat gcgctcgcga tttcccgcgc aacgctgcgc
    15241 aacatgaagc agaacctgct cggtgccttt atctacaaca gcatcggtat tccggtcgcc
    15301 gccggtattt tgtggccgtt tactggaaca ctgcttaacc cggtagttgc cggagcggca
    15361 atggcgctct cgtcgattac cgtagtgagt aacgccaacc ggttgctgcg gtttaaaccg
    15421 aaggaataag tacgtcattg cggatgcgct gctttagggc ggcgcatttg gcatcgacgc
    15481 atgactgccg gatgcggcgt aaacgcctta tccggcctac gagatgaagc gatgttttgg
    15541 ccttacgcga ccttttttgc acgctcttca cggcgtgcca aatattcagc cactgtttca
    15601 atccgtccca attcttcctg ggtgcgcatg ttgttttcaa cttcccaaag atcaactgcc
    15661 gcctggaggt ttagcctaaa atcgactgtg gtatcaaaaa ctttcgccag acggaatgcc
    15721 atctcagtgg tgagtttacg attgttattg atcagtgcac tgacgctatt acgatgaaca
    15781 tgcagcaact ctgctaactc attgattttc aaatcgagcg gttccagata ttcatagaga
    15841 agaatatctc cgggtgtcgt cggttttctt gttgcctgtt tcatggtttt taactgttgc
    15901 ttccctgtga atagcgatgc aagaacatac tgaatcatac aaacacatta aacaagtccc
    15961 caactccatt tgcgccatcc ctcaccgcgc gctagcatga tatttcacaa agggaggtcg
    16021 tatggatctg ttgtaccggg taaaaacgct ttgggccgcg ctgcgcggta atcattacac
    16081 ctggcctgcc atcgatatca ccctccccgg caatcgccat tttcatctga ttggcagtat
    16141 tcatatgggt agccacgata tggctcccct gcccacccgt ttgctcaaaa agctcaaaaa
    16201 cgccgatgcg ctgatcgtcg aggcggatgt ttccaccagc gatacgcctt ttgctaattt
    16261 gcctgcctgc gaggcgctgg aagagcgcat tagcgaggag caactccaaa atctgcagca
    16321 cattagccag gagatgggca tttctccctc actcttttct acccaaccgc tgtggcaaat
    16381 cgcgatggtt cttcaggcga cgcaggcaca aaaactgggg ctgcgggcag aatacggtat
    16441 cgattaccag ctattgcagg cggcgaagca acaacataaa cccgtgattg aactggaagg
    16501 ggctgaaaac cagattgcca tgttgctcca gctccctgac aaaggactgg cgctgctgga
    16561 cgatacgctg acccactggc ataccaacgc acggttgctg caacaaatga tgagctggtg
    16621 gctgaatgcg ccgccgcaaa ataatgaaat aacgctgccc aatacgttca gtcagtcgct
    16681 gtacgatgtg ctgatgcatc agcgaaatct cgcctggcgg gataaattac gcgccatgcc
    16741 gccggggcga tatgtggtcg cggtcggtgc actacacctg tatggagaag ggaatttgcc
    16801 gcaaatgttg cgctaaaaaa atggccaata tcgctattgg cccgtcaaag aggaatttca
    16861 tttttttatt attatgccgt cactttaagc gacggtgtaa ctcgattgtc gctcaatctc
    16921 accatcctgc caatactctt aggcaagatg actctttttt ttggaccgcc atcaggcgta
    16981 tgcttgcttc gtttttttgt ttaaggcagg aaaattacca tgacacccgc agttaaatta
    17041 ctcgaaaaaa acaagatttc gtttcaaatc catacctacg agcacgatcc ggctgaaacc
    17101 aattttggcg atgaagtcgt caaaaaatta ggtttgaatc cggatcaggt ctacaaaacg
    17161 ctgctggtgg cagtgaacgg tgatatgaaa caccttgccg tggccgttac gccggtcgca
    17221 ggtcaactgg atcttaaaaa agtagcaaaa gcgctgggtg ccaagaaagt tgagatggcc
    17281 gatccgatgg tcgcgcagcg ttcgacggga tacctggttg gggggattag cccactgggg
    17341 cagaaaaaac gtctgccaac gattatcgac gcccccgcac aagaatttgc cactatttat
    17401 gtttccggcg gcaagcgcgg actggatatc gaactggcgg caggcgatct ggcaaagatc
    17461 ctcgatgcca aatttgctga tatcgcccgc cgcgattaag cattgtgccg gatgcaaaca
    17521 tccggcactt tcggattact gccagctcac ctcacctttc ggttcataaa cactcacatc
    17581 cagcggcgag cttttctgga tatacgcttt cagcacttcg gcatcaataa agccggtatt
    17641 cacatagccc ggtttgttat caaggcgcgg atatccatca ccgccggtgg cattgaagtt
    17701 taatgtcgcc atacggtaag ttttcgccgg atcgaccggt tcgcctttga ttttaaggtc
    17761 gttcagtttg ccgtctttcg ccacaaagct aacgttggca aattgcgggt aggcacctga
    17821 atctggcttc atctgcgcga cggcggtcag gtaatcaatc acctctttac cggtcatgtc
    17881 ggcatacacc accacattgc cgaatggctg cactttcagc acgtttttat agctgatatc
    17941 gcctgcttcg atagaatcac gaattccgcc tccgctcatc accgcaaagt cggcaccagt
    18001 gcgatccatt tgggctgcca gaatcaaccg ccccatattg gtctgtacaa aacgcacttt
    18061 gtcacgatcg ccttccagac gaccattggt ttcgcctatt ttcacttcca gctgcgcttt
    18121 gcctttgttc tggaacggtg ataacagcga gatcatttgc tggttttcag cgatttcagg
    18181 agtgtaaagc acgcgctcgc ttttcccgtc ttcccaggtc actttcttct tcaggttcac
    18241 cggaatcagc tggtagttaa ccattttcat ttcgccatta cgaaactcaa aatcagcccg
    18301 tcccacgtat ttgccccact catgcgcctg cacaatccag atgccgtttt gttgatctgg
    18361 cttgcatggc gtacccggca cgtaatcgac ctgttttttg ttttctgccg ccatgcagac
    18421 cggatcttgc gagtgaccac cgacgatcat cgccagcgat ccggcaggca gcgcgcgtgc
    18481 catctccaca tcgcccggtg cgttagagcc gtgctcacca ttatcgtaat gccccatatg
    18541 ggtcgccgcg ataataatgt ctggcttttc tgtctgttgc agctcctgaa tcaccagctt
    18601 cgcttcatcg gcgggcttac gaaattcgac atcagtgaag tattccgggt taccaatttt
    18661 tgctgtgtca tcggttgtca gcccaatgac ggcaattttc agatcctgac gcttaaacag
    18721 cgcccacggt ttaaacaggc gctcgccagt acttttctgg tagatattcg cggaaagcaa
    18781 cgggaacttg gcccactttt cctgctggcg taatacggtg agcggattat caaattcatg
    18841 attaccgatc gccatcgcgt catagcccac cagattcata ccgcgaaaat caggttcggc
    18901 atcctgtaag tcagactcgg gcacgccagt gttaatgtcg ccaccggaaa gtagcagcac
    18961 gctaccgcct tcagccgcaa cctctttgcg gataccatcc accagcgttt tttgcgccgc
    19021 cagaccatat tcgccatatt cattgcgcca aaaatgccca tgatgatcat tggtatgcag
    19081 aactgtaatt ttgtaggttt tatcctgctc atacgccaga gcagtttcac tcgccagtgt
    19141 aaatgtggtt aacagcgcta acgccacgcc ccgctgcaat aatttcatac ttctctccct
    19201 gacctgattt caacgtgttg aaaaatagta tgacgcgaca tcatagacaa gtctgcgtcc
    19261 ggtgaagata aatctgcatc cagattagaa attctgtatt cggcgttgag acttccttca
    19321 ggcgacgcat acaggtatgt tagtcgggta ataatcacaa ataagatcat tctttacatc
    19381 gcaaccctga aaaagtgact ttcctatggc aatgagtgaa caaccccagc ctgtggcggg
    19441 cgcggctgcg tcaacgacca aggcccgaac atcgtttggt attttaggtg ctatcagcct
    19501 ctcacatctg ctgaacgaca tgatccaatc gctgattctg gcgatttatc cgctgcttca
    19561 gtcagaattt tctctgacat ttatgcagat tggcatgata accctcacct tccagctcgc
    19621 ctcttcgcta ctgcaaccag tggtcggcta ctggaccgat aaatatccga tgccgtggtc
    19681 gttgccaatt ggcatgtgct ttaccttaag tggtctggtg ctgcttgcgc tggcgggcag
    19741 ttttggcgca gttctgctgg cggcggcgct ggtcggtacc ggttcatcgg tctttcatcc
    19801 ggaatcttct cgcgtagccc gtatggcttc cggcgggcgg catggcctgg cgcaatctat
    19861 ctttcaggtc ggcggcaact ttggcagttc cctgggaccc ttgctggcgg cggtgattat
    19921 cgcgccttat ggcaaaggca acgttgcctg gtttgtgctt gcggcactgc tggcgatcgt
    19981 ggtgttggcg caaatcagcc gttggtactc ggcacagcac cgaatgaata aaggaaaacc
    20041 caaagcgacg attatcaatc cactgccgcg caacaaagtg gtactggcgg tcagcattct
    20101 gttaatcctc attttctcga aatatttcta tatggcgagc atcagcagct attacacctt
    20161 ttatctgatg caaaaattcg gattatctat ccagaatgct cagcttcatc tgtttgcctt
    20221 cctgtttgcc gttgcggcag gtacggtgat cggcgggcct gtaggggata aaattgggcg
    20281 gaaatatgtg atttggggct ctatcctcgg cgttgcgccg tttacgctga ttttacccta
    20341 cgccagcctg cactggacgg gggttttaac ggtgattatt ggatttatcc tcgcttcggc
    20401 attctctgcc attctggttt acgctcagga gctgcttccg ggacgtatcg gtatggtttc
    20461 tggactcttt ttcggttttg cttttggtat gggaggtctg ggagcggcag ttctggggct
    20521 tatcgccgat cacaccagta tcgagttagt ctataaaatc tgtgctttcc tgccactatt
    20581 ggggatgttg accatattcc tgcctgataa ccggcataaa gactgatttc attgcagcca
    20641 aaggcaaact ttggctgcat cgttttacag tcgccataag cctttcctct gttaaaccgc
    20701 cttctgtttt ccgatccgtg ctttttttgc agc
//
LOCUS       DAZFQD010000090        20722 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.090, whole genome shotgun sequence.
ACCESSION   DAZFQD010000090 DAZFQD010000000
VERSION     DAZFQD010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20722
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.090"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            149..925
                     /gene="exuR"
                     /locus_tag="ACHKTW_003290"
     CDS             149..925
                     /gene="exuR"
                     /locus_tag="ACHKTW_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HFO6464256.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            1165..1248
                     /gene="yqiM"
                     /locus_tag="ACHKTW_003291"
     CDS             1165..1248
                     /gene="yqiM"
                     /locus_tag="ACHKTW_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqiM"
                     /protein_id="HFO6464257.1"
                     /translation="MLMYQTRRTYQNSNNIAVVHLLKPAWR"
     gene            1270..1932
                     /gene="yqjA"
                     /locus_tag="ACHKTW_003292"
     CDS             1270..1932
                     /gene="yqjA"
                     /locus_tag="ACHKTW_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YqjA"
                     /protein_id="HFO6464258.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            1936..2319
                     /gene="mzrA"
                     /locus_tag="ACHKTW_003293"
     CDS             1936..2319
                     /gene="mzrA"
                     /locus_tag="ACHKTW_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HFO6464259.1"
                     /translation="MQIPRMSLRQLAWSGTVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            2466..2834
                     /gene="yqjC"
                     /locus_tag="ACHKTW_003294"
     CDS             2466..2834
                     /gene="yqjC"
                     /locus_tag="ACHKTW_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 family protein YqjC"
                     /protein_id="HFO6464260.1"
                     /translation="MKYRIALAVSLFALSAGSYATTLCQEKEQNILKEISYAEKHQNQ
                     NRIDGLNKALSEVRANCSDSQLRADHHKKIAKQKDEVAERQQDLAEAKQKGDADKIAK
                     RERKLAEAQEELKKLEARDY"
     gene            2872..3177
                     /gene="yqjD"
                     /locus_tag="ACHKTW_003295"
     CDS             2872..3177
                     /gene="yqjD"
                     /locus_tag="ACHKTW_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqjD family protein"
                     /protein_id="HFO6464261.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            3180..3584
                     /gene="yqjE"
                     /locus_tag="ACHKTW_003296"
     CDS             3180..3584
                     /gene="yqjE"
                     /locus_tag="ACHKTW_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFO6464262.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            3574..3873
                     /gene="yqjK"
                     /locus_tag="ACHKTW_003297"
     CDS             3574..3873
                     /gene="yqjK"
                     /locus_tag="ACHKTW_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HFO6464263.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSASRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            4059..4451
                     /gene="yqjF"
                     /locus_tag="ACHKTW_003298"
     CDS             4059..4451
                     /gene="yqjF"
                     /locus_tag="ACHKTW_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFO6464264.1"
                     /translation="MKKLEGVGVLVARILMPILFITAGWGKITAYSGTQQYMEAMGVP
                     GFMLPLVILLEFGGGLAILFGFLTRTTALFTAGFTLLTAFLFHSNFAEGVNSLMFMKN
                     LTISGGFLLLAITGPGAYSIDRLLNKKW"
     gene            4521..5507
                     /gene="yqjG"
                     /locus_tag="ACHKTW_003299"
     CDS             4521..5507
                     /gene="yqjG"
                     /locus_tag="ACHKTW_003299"
                     /EC_number="1.8.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
                     /protein_id="HFO6464265.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYISLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DALGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVAKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTGIISIGPWQDLDEPHGRDVRFG"
     gene            5800..6165
                     /gene="yhaH"
                     /locus_tag="ACHKTW_003300"
     CDS             5800..6165
                     /gene="yhaH"
                     /locus_tag="ACHKTW_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFO6464266.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTTIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEP"
     gene            6407..6763
                     /locus_tag="ACHKTW_003301"
     CDS             6407..6763
                     /locus_tag="ACHKTW_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFO6464267.1"
                     /translation="MQWYLAVLKNYVGFSGRARRKEYWMFTLINAIVGAIINVIQLIL
                     GLEFPFLSLIYLAATIIPVIALCVRRLHDTDRSGAWALLYLVPIIGWLVLFVFACLEG
                     NSGSNRYGNDPKFGSN"
     gene            complement(6814..7710)
                     /gene="yhaJ"
                     /locus_tag="ACHKTW_003302"
     CDS             complement(6814..7710)
                     /gene="yhaJ"
                     /locus_tag="ACHKTW_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HFO6464268.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGAWERLEQGRADIVIAPDMHFRSSS
                     EINSRKLYTLMNVYVAAPDHPIHQEPEPLSEVTRVKYRGIAVADTARERPVLTVQLLD
                     KQPRLTVSTIEDKRQALLAGLGVATMPYPMVEKDIAEGRLRVVSPESTSEIDIIMAWR
                     RDSMGEAKSWCLREIPKLFSGK"
     gene            7815..8516
                     /gene="yhaK"
                     /locus_tag="ACHKTW_003303"
     CDS             7815..8516
                     /gene="yhaK"
                     /locus_tag="ACHKTW_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HFO6464269.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDACPQRENPLIQKLALNMGKQQLIASPEGTMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            8539..8703
                     /gene="yhaL"
                     /locus_tag="ACHKTW_003304"
     CDS             8539..8703
                     /gene="yhaL"
                     /locus_tag="ACHKTW_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhaL"
                     /protein_id="HFO6464270.1"
                     /translation="MSKKSAKKRQPVKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(8915..10225)
                     /gene="cyuA"
                     /locus_tag="ACHKTW_003305"
     CDS             complement(8915..10225)
                     /gene="cyuA"
                     /locus_tag="ACHKTW_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfidase"
                     /protein_id="HFO6464271.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDSTAQ
                     AIADAKALLAAGKVSVKIQEPCNEILFSRAKVWHGEKWACVTIVGGHTNIVHIETHNG
                     VVFTQQACVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCERGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITATMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(10253..11584)
                     /gene="cyuP"
                     /locus_tag="ACHKTW_003306"
     CDS             complement(10253..11584)
                     /gene="cyuP"
                     /locus_tag="ACHKTW_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026203.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFO6464272.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTGMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSPFLAFS"
     gene            complement(11859..13223)
                     /gene="tdcG"
                     /locus_tag="ACHKTW_003307"
     CDS             complement(11859..13223)
                     /gene="tdcG"
                     /locus_tag="ACHKTW_003307"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFO6464273.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEALLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFTRIWQVMHD
                     GIERGMNTEGVLPGPLNVPRRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(13295..13684)
                     /gene="tdcF"
                     /locus_tag="ACHKTW_003308"
     CDS             complement(13295..13684)
                     /gene="tdcF"
                     /locus_tag="ACHKTW_003308"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HFO6464274.1"
                     /translation="MKKIIETQRAPSAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(13698..15992)
                     /gene="tdcE"
                     /locus_tag="ACHKTW_003309"
     CDS             complement(13698..15992)
                     /gene="tdcE"
                     /locus_tag="ACHKTW_003309"
                     /EC_number="2.3.1.-"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HFO6464275.1"
                     /translation="MKVDIDTSDKLYADAWLGFKGTDWKNEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREIDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKSGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(16026..17234)
                     /gene="tdcD"
                     /locus_tag="ACHKTW_003310"
     CDS             complement(16026..17234)
                     /gene="tdcD"
                     /locus_tag="ACHKTW_003310"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006082 - organic acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HFO6464276.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVEIDTEMNNRSNSCGERIVSSENARVICAVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(17260..18591)
                     /gene="tdcC"
                     /locus_tag="ACHKTW_003311"
     CDS             complement(17260..18591)
                     /gene="tdcC"
                     /locus_tag="ACHKTW_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HFO6464277.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLILKFGYKGDKTKVSLGKLNTISMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(18613..19602)
                     /gene="tdcB"
                     /locus_tag="ACHKTW_003312"
     CDS             complement(18613..19602)
                     /gene="tdcB"
                     /locus_tag="ACHKTW_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HFO6464278.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(19701..20639)
                     /gene="tdcA"
                     /locus_tag="ACHKTW_003313"
     CDS             complement(19701..20639)
                     /gene="tdcA"
                     /locus_tag="ACHKTW_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HFO6464279.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVG"
BASE COUNT         5348 a         5399 c         4922 g         5053 t
ORIGIN      
        1 gacgatactt attttttagg aatttataaa tattcacaat attaattcaa tacattaatt
       61 caatcaccaa atttatatat gaccaatggc atacccatga ataaagtggt atgataactt
      121 cgtccgcatt ccactccggg agacgcatat ggaaatcact gaaccacgcc gtttgtatca
      181 acaacttgcc gctgacctga aagagcgcat cgaacagggc gtctatctgg taggtgataa
      241 actgcctgca gaacgcttta ttgccgatga aaagaacgtc agccgtacgg tagttcgtga
      301 agccatcatc atgctggaag ttgaaggcta tgtcgaagtg cgtaaaggtt caggtattca
      361 tgtggtttcc aaccagccgc gccatcagca ggcggctgac aataatatgg aattcgccaa
      421 ttacggtccg tttgagttgc ttcaggctcg ccagctcatc gaaagtaata ttgccgagtt
      481 cgcggcaact caggtaacga aacaagacat catgaaactg atggccattc aggaacaggc
      541 gcgcggcgaa caatgcttcc gtgattccga gtgggattta cagttccaca ttcaggtagc
      601 cctggcgacg cagaactccg ccctggcggc catcgttgaa aaaatgtgga cccaacgtag
      661 ccataacccg tactggaaaa aactgcacga acacattgat tcccgtaccg tcgataactg
      721 gtgtgatgac cacgatcaaa tcctcaaggc gctgattcgc aaagatcctc atgctgctaa
      781 gctggcaatg tggcagcacc tggaaaacac caagatcatg ttattcaacg aaaccagcga
      841 cgacttcgag ttcaatgccg accgctatct gttcgccgaa aacccggtag tgcatctcga
      901 tacggccacc agcggcagta aatgaaatac ctgtccgaca ggcgctgcga cgcgcctgtt
      961 tgtctcgtcg gtaagcgaaa agtataaagt gtcagcctgt gtaaatcctc tcgccaccct
     1021 cccctgcatt cagcaaaatc agactccacg gacatgtaat tttgataacg aacaacgttg
     1081 acctttgtta caattagatt caatttgaat ttatgttttt gaatgctttc ttatctcacg
     1141 atttaacagg gaatagttca ggctgtgttg atgtatcaaa cccgcagaac ataccaaaac
     1201 agcaataaca ttgcggtagt gcatctttta aaaccagcgt ggcgttaacc gattcaccag
     1261 gaataatgaa tggaactttt gacccaattg ctgcaagccc tgtgggcgca ggattttgaa
     1321 accctggcca atccatcgat gattggcatg ttgtattttg tcttgtttgt aattttgttc
     1381 cttgaaaacg gcttgcttcc agcggccttt ttaccgggcg acagtttact ggtattggtc
     1441 ggcgtgttga ttgcgaaagg cgcgatgggt tacccgcaaa cgattctgct gctgaccgtt
     1501 gccgccagcc tcggctgctg ggtcagctat attcaggggc gatggctggg caatacccgc
     1561 accgtacaaa actggctatc tcatttaccc gcgcattatc atcaacgcgc acaccatctt
     1621 tttcataaac acggtttatc ggcgctgtta attggtcgct ttattgcgtt tgtcagaaca
     1681 ctgctgccga cgattgccgg gttatcaggg ctgaataacg cgcgctttca gtttttcaac
     1741 tggatgagcg gtctgctgtg ggtattgatc ctgacaactc tgggttacat gctcggcaaa
     1801 acgccggtat ttttaaagta cgaggaccag ctgatgtcat gcctgatgct gctcccggtg
     1861 gtgctgctgg tgtttggcct ggcaggttct ctggtcgtgt tatggaaaaa gaaatatgga
     1921 aatcgggggt aagggatgca aatacctcgc atgtcgcttc gccagctagc ctggtccggc
     1981 actgttttac ttctggtcgg cacgctgttg ctggcctggt ccgcggttcg ccagcaagag
     2041 tctacgctgg cgattcgtgc cgttcatcaa ggcacaacga tgccagacgg tttttcgatc
     2101 tggcatcacc ttgacgctca tggcattcct ttcaaaagta tcacccccaa aaacgacact
     2161 ctgttaatta catttgactc cagcgaccag agcgccgccg caaaagcggt cctcgacaga
     2221 acattgcccc atggctacat cattgcgcag caggacaata acagtcaggc tatgcagtgg
     2281 ctgacccggt tacgggataa ctctcatcgc ttcggataac ttcctgttat ccgaaacata
     2341 tcactcactt tggtgatttc accgtaactg tctatgatta atgaggcggt ggttaacact
     2401 accgcttcgt tgtctggaca atcgttcctt tgtaataggt ccgaatcaca atggaaggtt
     2461 caagaatgaa ataccgcatc gctttagctg tttctctctt tgctcttagt gccggtagtt
     2521 atgccactac cctgtgtcag gaaaaggagc aaaatatcct taaggagatc agctatgccg
     2581 aaaaacacca aaaccagaat cgtattgacg gtctgaataa agccctgagt gaagtccggg
     2641 ccaactgttc agatagccag ctgcgtgccg atcatcacaa gaaaatcgca aagcagaaag
     2701 atgaggtggc ggaacgccag caagatttag ccgaggcgaa gcaaaaaggc gatgccgata
     2761 agattgccaa acgcgaacgg aaactggcag aagcgcagga agagctgaaa aagctggaag
     2821 cgcgcgacta ctaactcaca atagtcacta cttactcacc tggagaaaac tatgtcgaaa
     2881 gaacacacta cggaacatct gcgtgctgag ttgaaatccc tttccgatac gctggaagag
     2941 gtgcttagct catctggcga gaagtcgaaa gaagagttga gtaagattcg tagcaaagcg
     3001 gagcaggcac tgaaacagag ccgttatcgc ctgggtgaaa ccggtgatgc catcgccaaa
     3061 caaacccgtg tcgcggcggc gcgtgctgat gagtatgtgc gtgaaaatcc gtggaccggc
     3121 gtgggcattg gcgctgcaat cggtgtagtg ctcggcgttc tgctgtcgcg tcgttaatta
     3181 tggcggacac tcatcacgcg caagggcccg gtaaaagcgt tctgggcatc gggcagcgaa
     3241 ttgtttccat catggttgaa atggtggaaa cacgtctgcg gctggcggtg gtggagctgg
     3301 aagaggaaaa agctaatctc tttcaacttt tactgatgct gggcctgacg atgcttttcg
     3361 ctgcatttgg tcttatgagc ctgatggtgc taattatttg ggcggttgat ccgcaatatc
     3421 gcctgaatgc gatgattgcc accaccgtgg tgttgctgct actggcactg attggcggta
     3481 tctggacgtt acgtaaatcg cgtaaatcca ccttgctgcg ccatacacgc catgagctgg
     3541 caaacgatcg gcaactgctc gaggaggagt cccgtgagca gtaaagtcga acgtgaacga
     3601 cgtaaggcgc aactgcttag ccagatccag caacaacggc tggatctttc cgccagtcgt
     3661 cgtgaatggc tggaggcaac aggcgcttac gatcgtcgct ggaatatgct gctaagtctg
     3721 cgctcctggg cgctggttgg cagtagcgtg atggcgatct ggacgattcg ccatcctaat
     3781 atgctggtcc gctgggccag acgcggtttt ggcgtatgga gcgcctggcg tctggttaaa
     3841 acgaccctca agcagcaaca gcttcgcggt taacttccct ctggccggag caattccggc
     3901 cttatccctc aaattttttg aagatttttg acagttttcc ttgctaacaa tcatcattca
     3961 ccacgtttat gattctctcc atcgacagca acgacgctaa taccgcgccc ttgcacaaaa
     4021 aaacaatcag cagcctgagt ggcgcagtgg agagtatgat gaaaaaatta gaaggtgttg
     4081 gtgtactggt agcgcgcatt ttaatgccga ttctgtttat taccgcaggc tggggaaaaa
     4141 tcaccgctta ctctggtacc caacaatata tggaagcaat gggtgtcccg ggttttatgc
     4201 tgccgctggt gattctgctt gagttcggtg gtggtctggc aatcctgttc ggtttcctga
     4261 ctcgcaccac agccctgttt actgcgggct ttacgctgct gacggcattt ttatttcaca
     4321 gcaactttgc tgaaggcgtc aactcgctga tgttcatgaa aaacctgaca atttctggcg
     4381 gattcctgct gctggcaatt accggtccgg gcgcgtatag catcgaccgc ctgctgaata
     4441 aaaagtggta agcacgcgct atacttaacg ataaaaaacg aggaggaagc tcctcgtttt
     4501 tgctattgga ggagagaaaa atgggtcaac tgattgacgg cgtctggcat gacacctggt
     4561 acgataccaa atctaccggc ggtaaatttc aacgttcagc ttccgcattt cgtaactggc
     4621 tcactgccga tggcgcacct ggccccactg gcacaggcgg ttttatcgca gagaaagatc
     4681 gttatcatct ctatatttca ctcgcctgcc cgtgggcgca ccgcacgctg atcatgcgca
     4741 aactcaaagg actggaaccg tttatttccg tttccgtagt gaacccgctg atgctggaaa
     4801 acggctggac ctttgatgac agttttccgg gagcaaccgg agatacgctc tatcaacatg
     4861 aatttctgta tcagctttat ctccacgccg atccacacta cagcggacga gttactgttc
     4921 ccgtgctgtg ggacaaaaag aaccacacca tcgtcagcaa cgaatcagca gaaatcatcc
     4981 gcatgttcaa taccgcgttt gatgcgctgg gcgcaaaagc gggtgattac tacccaccag
     5041 ccctgcaaac gaaaattgac gaacttaacg gctggattta tgacaccgtt aacaacggcg
     5101 tgtataaagc tggttttgcc accagccagc aagcctacga cgaggcggtg gcgaaagtgt
     5161 ttgaatcgct ggcgcgactg gagcagattt taggccagca tcgttacctg accggcaacc
     5221 agctaaccga agccgatatt cgcctgtgga ccacgctggt ccgttttgat ccagtgtatg
     5281 tgacccactt caagtgtgat aagcaccgca tcagcgatta ccttaatctg tatggcttcc
     5341 tgcgtgatat ctaccagatg ccgggaattg ccgaaacagt caatttcgat cacatccgta
     5401 atcattactt ccgcagccat aagaccatca atcctacggg gattatttca attggtccat
     5461 ggcaggatct cgatgaaccg catggacgag atgttcgctt cggttaaaaa aaggcacctt
     5521 acgaggtgcc tctgataatt agctaagtgc atgttgttca tgtcggatgc ggcgtgaacg
     5581 ccttatcaaa cctacaaaac atttatattc aatatgttgc aatgaccgcg caggccagat
     5641 aagcgtagcg catctggcga ttttgcgctt gtcatcagtc ttaacgcgcc ttcacagcac
     5701 tttattaatt ccttgaacga atatttactg ccatttcatt cacgtctatt cttaatttgc
     5761 tgctttaaaa acaagtgatt gagcaaaatt gaggcaaaaa tggactggta tctgaaagta
     5821 ctaaaaaatt atgtcggttt ccggggtcgt gcgcggcgca aagagtactg gatgtttatt
     5881 ctggtcaaca tcatctttac gttcgtgctg gggctgctgg ataaaatgtt aggctggcaa
     5941 cgcgctggtg gcgaaggcat cctgacgaca atctacggta ttctggtgtt tttaccgtgg
     6001 tgggcagttc agttccgccg cctgcacgac accgaccgct cggcgtggtg ggcactgttg
     6061 ttcttaatcc cgtttatcgg ctggcttatt atcatcgtct ttaactgcca ggcgggtacg
     6121 ccaggcgaaa accgctttgg accagatccg aaactggaac cttaaaacaa atctgctcgc
     6181 cggatgtctg actcatccgg cttttatcat catgttgatg aatgaataat ttactaatgt
     6241 ttatttaaaa tatttcaatg tcgttatata aattacctat aaaaaacaac catgtattta
     6301 tcttaaatat caaaaccttt ataacatccg aaaaagaaag atacgaatag catttacaca
     6361 atacccggaa tagactttcg tcacttttaa ttaaagggat gtttttatgc agtggtattt
     6421 agccgtatta aaaaattatg ttggtttctc tggtcgcgca cgtcgtaaag agtactggat
     6481 gtttactctg attaacgcca tcgtcggcgc tattatcaat gtcattcaat tgattttagg
     6541 tttagagttt ccattccttt ctctcattta cctggcagca acaatcattc cggtcattgc
     6601 tctgtgtgtt cgtcgtctgc acgatactga ccgttcaggt gcgtgggcgc ttctctatct
     6661 ggtaccgatc attggctggt tagttctgtt tgtatttgcc tgtttagaag gcaattctgg
     6721 cagcaatcgt tacggaaacg atcctaagtt tggttcaaat taatcttaga attggggcga
     6781 catttcgccc ctttttatta ataataacaa atattatttt ccgctaaaaa gtttgggaat
     6841 ttcccgcaga caccaggatt tcgcttcccc catactgtca cgacgccagg ccataataat
     6901 atcgatctcg ctggtcgatt ccgggctgac gacacgcaac cgcccttccg caatatcttt
     6961 ttcgaccatc ggatacggca tcgtcgccac gccaagcccc gccagtaatg cctgacgttt
     7021 atcttcaatc gtgctcaccg ttaagcgcgg ctgtttgtcc agcagctgta cggtcaacac
     7081 cgggcgctca cgagcggtat ccgctaccgc aattccacga tatttcacgc gcgtcacttc
     7141 agataacggt tccggctcct gatgaatcgg gtgatcaggc gcggcaacgt agacgttcat
     7201 taaggtatag agcttgcgcg agttgatctc cgacgaggaa cgaaaatgca tatccggcgc
     7261 gataacgata tccgcccgcc cctgctccag tcgttcccac gcccccgcca gcacttcggt
     7321 gatgattgcc agttgggtat tggcttttgc cgccagtttg tcgattaacg ggaaaaaggc
     7381 aggtgtcggt accagcgcct cggtcacaat ggtgagatgc gtttcccaac cgcgcgcgag
     7441 ggcttccgca tcggtagtca gtttatctgc ggcttccagc aaaacgcgcc ctcgctccag
     7501 caacatcctc ccgacattgg tgaatttggt gcgatggccc gagcggtcaa acagcaccac
     7561 atccagctct tcttccagtt tttgcatggt gtagctaagt gcggaaggca cgcgtcccag
     7621 ctcatccgcc gccgccgcaa aactgccccg gcgatcgatc gcatccataa cccgtagtgc
     7681 ttccagcgtt aatgcccttt ctttggccat ttcgttctca ttcaggaaat ttgaacatac
     7741 cgggcagaat atctggctaa caatgtagcg tccagcccct taccataaaa ggaagtaaag
     7801 agaggtcaag aattatgatt actacccgaa ctgccagaca gtgtggacaa gcagactacg
     7861 gatggttgca ggcccggtat actttttcct ttggacacta cttcgacccg aaattgttag
     7921 gctatgcctc cctgcgcgtg cttaaccagg aagtgctggc cccaggtgcc gcctttcagc
     7981 cgcgaaccta tcccaaagtc gatattttaa atgtgattct ggatggagaa gcagagtatc
     8041 gcgacagcga aggcaatcat gttcaggcca gcgccggtga ggcgttgctg ctctctaccc
     8101 agccgggtgt cagctacagc gaacacaatc tcagcaaaga caaaccgtta acacgaatgc
     8161 agctttggct ggacgcctgc ccgcagcgag agaatccgct gattcaaaaa ctggcgctta
     8221 atatgggcaa gcagcaatta atcgcctcgc cagagggtac gatgggaagc ctgcaattac
     8281 gccagcaagt gtggctgcac catatcgtgc tcgacaaagg cgaaagtgcg aatttccagt
     8341 tgcatgggcc acgcgcgtat ttgcaatcga ttcacgggaa atttcatgcg cttacgcatc
     8401 atgaagagaa agcggcgctg acctgcggtg atggggcgtt tattcgtgac gaggctaaca
     8461 ttacgctggt tgccgattcc ccactgcgcg ctttgctgat agatttgcct gtctagttgt
     8521 ttttacaggg agatgatgat gagtaaaaaa tcggccaaaa agcgccagcc agtgaagccc
     8581 gtggtggcga aagaacctgc tcgcaccgcc aaaaattttg gctatgaaga gatgttgagc
     8641 gagctggaag ccatcgtcgc ggatgctgaa acgcgtttag ccgaggatga agctaccgcg
     8701 taaagacagg ttgccggatg cggcgaaaac gccttatccg gcctacaaaa tccaacaaca
     8761 ttaacgaatt aaacaacccg taggccggat aagatgcgcc agcatcgcat ccggcatcag
     8821 gtacaaaaca caacaacatc aacaagttga gcaccccgta ggccggataa ggtgcgccag
     8881 catcgcatcc ggcacaatac tcaaaacctg gcgtttatct ggccttgctc gccataatct
     8941 cgataatctg ccgatccgtt tgctgcatcg aatggcttgc taacgcacac aggttggcaa
     9001 tcgactgctc aacatcatgc gccacgatcc cttcattgcc ggtcacggcg gtatcatcca
     9061 gcgccattaa caccgctttc cacgcagccg aagcactggt cgaaaccttc atcgcgcagc
     9121 tgttcgacgc accatcgcaa atcatgccgc tgacatcgcc gatcatactg ctgatcgcca
     9181 tcgagatggt ttcataacgc ccatccacca gccatgccat cccggcggcg gcccccattg
     9241 ctgcggtcgt tgcggcacac agcgcagaca aacgcggtaa ctggttatgg atgtaaattg
     9301 cgctcaaatg agaaagcatc agcgcacgcg ccagccgttc atcatccgct ccgaagtgtt
     9361 ctgctaccac caccacaggc attgttgcgg tgatcccctg gttacccgag ccggagttac
     9421 tcatagccgg aagcgtagcg ccgcccatac gcgcatcgga tgccgcgctg gtacgaatca
     9481 caatggatga agagagatct ttcgccagca aaccgcgctc gcactgtttt tccagcgtcg
     9541 cgccaatatg cagcccccac ttaccgctca aaccttcctg cgataacgca caatttagct
     9601 tcgcggaatc gagaataaag cggatcgccg caaacgggac ttcattgacg aacttcagga
     9661 tctcagccag cgtcgttctg gaaagcaccg tcagcggaga ctcttgctcg ccctctgcca
     9721 cacacgcctg ctgggtaaac accacaccat tgtgcgtctc aatatgcaca atgttggtat
     9781 gcccgccgac gatggtgaca cacgcccact tctcaccgtg ccagactttg gcgcgtgaga
     9841 agaggatttc attgcaaggt tcctggatct taacggagac tttccccgcc gccagcagtg
     9901 ctttggcatc ggcaattgcc tgcgcagttg agtctttcag cacttccagc ccggcgttgg
     9961 catttccacc taacgccccc agcgccgccg caatcggcag ccccaccatt cccgtgccgg
    10021 gaacggtgac gcccagaccg ttcttcatca gatttggcga aacccaggct tctacacgtt
    10081 caaccggacc ttccagttct gccgcagcaa ccgccgccgc cagcgccagt gaaatcggtt
    10141 cagtacatcc cagcgccggt tttacttcct cctgaactgc aaggatgtaa cgctgccata
    10201 acggatttaa agtcgaatca aacatattaa aaaccttaaa atttcaggta aatcaagaaa
    10261 atgccaggaa cggagaaaca caaagcaaca aaccagtgac gataatcagg tacagagaca
    10321 ttcctttgta tttgtgcaat gccggtactt tgtaaaccag ccacgccggg atcaggcacc
    10381 ctaccatgcc gaaaatcggg ctacagatag aggtgaagct caacaccggt gcgttcagta
    10441 cgatggcgct ccaggccagc aaaatggcga aaatcatgat gccgcgctga acaagatttt
    10501 cgttaatctt ctcggcaggc atcttgcgac gcaggatgtt cattacgatc ccttgcgttg
    10561 cttcgcgaaa gcctaaatag acgccaaaga atgcagtcat gacagcaaag atattgagaa
    10621 tgacgctgac cactttcacc cacgctgcgc cgtcaccgct aataaactgt gcggcaatcg
    10681 ccagcgcgga aatattctgc tcataggctt ttaccgcttc gtcatgtccc atcgccagcg
    10741 tgaacgacac ggcgtagaaa aagacggtga caaacaaaat gccaaacgcg atattcattg
    10801 cccgcaatgc tttatgccgc gccacttcaa ttgatttttc ccgcgagcga taagagatca
    10861 ccatcgggct taacgtctgg ataaacagaa tcgacgtcag ggtaaacggc agcgtaataa
    10921 tggcgttttt caccagcagc cccagcggcg gtagcgaacc gacgttatac agatgccaca
    10981 ttcccaccat cgacacgccc agcgccgcga ccaccagcag cttggtcagc accatgccgg
    11041 tcgaaatttt gaataacaat ttctcgccgc gtgaagagat cgccaccaga atgcaaatca
    11101 gtaccagacc ataaaaggga ctgtctgaca gcaacccttc cgtcacgccg aaggtatgca
    11161 gataggaagc actatcgttg gtgatggcgg tggaatagac gaacatccaa atcaccagca
    11221 ttacgaaata gagcgcacct aacaggatgc cccagttttt acctaaataa ccgctaatga
    11281 cactcgggta atctttacat tctggtgatt ctgccagcgt attaataaac aaccgctgaa
    11341 acagatacat tgccgggtaa ccaatcaccg atgagagcaa aaatacccac aatcccatca
    11401 aaccgacctg caccgggaga aaaacaatcc ccgcgccaat cgccatcccg atactcataa
    11461 tcacccagcc ggtgtcagta ctgtcgaatt tgatcgcttc tcgccactcg ctctcactca
    11521 ttccagcacg acccgccggg gtcgaagcgt ctgcaatgac gcctttattc gatgcaattt
    11581 ccataatttc tcgctcaata ttttgtaggg cttattattt ttttccgagc cgcatcaagg
    11641 cgatatgcgg tctacgtttt tgcaggcgag ttattagaat aaagaaatga tacgcgcgag
    11701 gtcagagaaa atcttcacaa tcaagacttt gaaataacgg agatggataa gaattttcta
    11761 ctaaattaat cgcaagagaa tattcattct ctatttgcgg cagatcacaa aaaaaaggtg
    11821 cacatttgtg cacccaagga tgaaagctga cagcaatgtc agccgcagac cactttaatg
    11881 gccagtcctc cgcgtgatgt ttcgcggtat ttatcgttca tatctttgcc ggtttcatac
    11941 atcgtctcga tcactttatc gagtgaaaca cgcggtgccg aggtgcggcg catcgccatc
    12001 cgcgcggcgt ttactgcttt cacggcatta atggcattac gttcaatgca cgggatttgt
    12061 acctgtccgg caaccggatc gcaggtcagc ccaaggttat gctccatcgc gatttccgcc
    12121 gcattgcata cctgcgccgg actgccgccc agtagttcag ttaaccctgc cgccgccatt
    12181 gaacaggcca cgccaatctc cccctgacag ccgacctccg cgccagagat ggaggcgttc
    12241 attttataca gcgcgccaat agcccccgct gccagaaaat agcgggcaat tgaccgctcg
    12301 tttaccggac gacggaactt atcgtaataa gccagcactg ccggaataat gccgcacgca
    12361 ccgttagtcg gtgccgttac cacgcgcccg ccagctgcgt tttcttcact aaccgccagc
    12421 gcgtacatgt tgatccagtc gatgacattc atcggatcgt tagagatgtt atcgctagaa
    12481 accagctgac gacgcagcgc tacggcacga cgcggcacat tgagcggacc aggcagcacg
    12541 ccttcagtgt tcatcccgcg ttcaataccg tcatgcatca cttgccagat acgggtaaac
    12601 cctgcgtcaa tttccgcttt actgcgcagc gctagctcgt tgtgcatcat caggccagat
    12661 atagacaggc cgttgtaatc acacattttc agcagttcac ctgctgagtg gaaatcgtaa
    12721 ggtacggacg tttcgacatc gtgcgacagg ccgaagtgtt cttcttcgac aataaacccg
    12781 ccgccgacag agtaataggt tttacttaat agcgcttcct gtcccttcca ggcagtgatc
    12841 cgcattccgt tctcatggcg aggcaacatt tcgggatgga agataatgtt ctttgctaca
    12901 ggaaaatcaa caatatgcgc accagatgcc actggcagcc gcccgctgcg cgttactaac
    12961 tctataaatg cagggatctc atcaatgaca acatcctgcg gactgtttcc tgccagtccc
    13021 atgataatgg cgacatccgt ggcatggcct ttgcccgtca gtgacaacga cccatacaga
    13081 tcgaccacaa tatggctcgt cgcggttaat aagccgctac tttccagccg atcaataaaa
    13141 ctttttccgg cattcattgg ccccacggta tgcgaactgg agggaccaat cccaattttg
    13201 aaaatatcga atgcactaat catatccaca ccctcggatt gccgttcagt gaagtggagc
    13261 ggaacgacct tgcgaccgtc ccgctcacga ggctttacgc actacgtact gcgatagctt
    13321 caatttccag cttcacatct ttcggcaaac gcgcaacctg cacacagctc cgggtcgggt
    13381 aggtcgcctg atgctcatcg aagaactgct tatagacttc attgatggtg gcaaaatcat
    13441 tcagatcggt gatgaacacg gtcatcttga tgatatcgcc cacgctcagc ccggcagcaa
    13501 ccacgatcgc tttgacgttt tcgaggctta aacgcgcctg atcttgcaca tcagccggga
    13561 tctcaccggt ctgcgggcaa accggaattt gcccggaggt gaagaccatg ctgcctaaat
    13621 caacgccctg aacataaggg ccgattgcgc ttggggcacg ttgcgtttcg ataatctttt
    13681 tcatacatcc tccggcgtca gagcgcctgg gtaaaagtac gtgaaataac atcctgttgc
    13741 tgttcacggg tcagtgcgtt gaagcgcacg gcgtagccag agacacggat tgtcaggtta
    13801 ggatattttt ccgggtgctc gatggcatcc agcagcattt cccgattcat tacgttgacg
    13861 ttgaggtgtt gaccgccttc gacatccgct tcgtggtgga aatacccatc cagcaggccg
    13921 acaaggttgg ttttacgtac tggatcttct ttgcccagcg ccgcaggaac gattgagaag
    13981 gtgtacgaga tcccatcttt ggcgtaggtg aacggcagtt tcgccaccga cgtcaatgag
    14041 gccacggcac ctttgcggtc acgaccatgc atcgggttag cgcccggcgc gaacggtgtt
    14101 ccggcgcgac gaccgtccgg cgtattaccg gttttctggc cgtacaccac gttagaagtg
    14161 atagtcagaa tcgactgggt agggacggcg ttgcgatagg ttggcagcgc tttaattttc
    14221 ttcataaagc gttcaaccag gtcgcaggca atgctgtcta cgcgctcgtc gttgttgccg
    14281 tactgcggat attcaccgtc gatttcaaag tccaccgcta ggccgttttc gtcacggatt
    14341 ggtttcacgc gggcatattt gatggcagac agggagtccg tcgccaccga caggcccgcg
    14401 atgccgcatg ccatagtgcg atagacatca cgatcgtgca gcgccatcag cgaagcttcg
    14461 tagctgtact tgtcgtgcat gtagtgaatg atattcagcg cgctgatgta ctgcaccgcc
    14521 agccagtcca tgaagtgatc gaggctgtcc atcactttgt cgtaatccag cacgtcgtcc
    14581 atcagcggtg ctgttttcgg cccgacctga atcttcagct tctcgtccac cccgccgtta
    14641 attgcgtaga gcagcgtttt cgccaggtta gcgcgtgcac caaagaactg catttgctta
    14701 ccaatcacca ttgggctgac gcagcaggca atcgcgtaat cgtcgctgtt gaagtcagta
    14761 cgcatcagat cgtcattttc atactgcaag gaagaggtga cgatcgacac ctgcgcggca
    14821 tattttttga aggcaatcgg taattcttcc gaccaaagaa tggtcaggtt aggttccggt
    14881 gccggcccca tagtgtgcag ggtgtgcaaa tagcggaagg agtttttggt caccagcgta
    14941 cgaccgtcca gccccatccc gccgatcact tccgtcgccc agattgggtc gccggagaac
    15001 agcgaatcaa attccggtgt acgcaggaag cgtaccatac ggatcttcat gatgaagtga
    15061 tcgatcagtt cctgtgcctg ctgctcattg agtacgccag atttaaagtc gcgctcaatg
    15121 tagatatcga ggaacgatgc cgtgcggccc agcgacatcg cgccgccatt ttgcgatttc
    15181 actgccgcca gataagcgaa gtagagccac tgcaccgctt cctgcgcatt ctgcgccggg
    15241 cgagagatat caaagccata tttcgccgcc atttcctgaa tctgcaacag cgcatgacga
    15301 tgctctgcca gctcctcacg cagacggatg gtggcttcca gatcctcgcc tttttccaga
    15361 cgagactgga gatcggcaaa ttgcagttcg cgttcacgta ccagataact gatgccatac
    15421 agcgctacgc ggcgatagtc accgataatg cgcccacggc catagccatc tggtaaaccg
    15481 gtcagcacgc cagatttacg gcagcgcagc atatccggtg agtaaacatc aaatacgccc
    15541 tggttatggg ttttacgcag atcggtaaac agatattcaa attcactgtc tatttcacgg
    15601 ccataggcgt ggaatgaact tttaatcata ttaatgccac cgaacgggtg tagcgcacgt
    15661 ttcaacggcg catccgtttg caggccaaca attttttcca gcggctggtt aatatatccc
    15721 gcatcatgag cggtaattgt ggtggcaata ttggtatcga aatcaaccgg cgcgtgggtt
    15781 gcgttttcga tacggatacc ttccattact ttttcccaca attccgtggt ggcaggcgtc
    15841 gcttcggcga ggaaagattc atcgccttca tacggtgtat agttatgttg aataaaatcg
    15901 cggacattaa tttcgttttt ccagtccgta cctttaaagc caagccatgc gtcggcgtac
    15961 agcttatcgc tggtatcaat atctaccttc atgaaaaata atctctctac aatacttcaa
    16021 ctaaattatg caaattctgc gggcgcgtta actttgccta aatgaatggc atccaaagca
    16081 atcatttttt cttcgttagt cggaataaca gcgcaaatga cacgcgcatt ttcactggaa
    16141 acaattcgct caccacagga gttagagcga ttattcattt ctgtatcaat ctctacgcct
    16201 aatacagcca aatgttccat gaccagacga cgaattaagc ttgaattctc tcctattccg
    16261 ccggtgaata taattccatc caggcgacgt aatgaagctg cgtgtccggc aatatgacgg
    16321 gcaattcggt gaacaaaggt tttaattgcc agttgcgcgc gttcgtgacc ttcatgccag
    16381 gctttttcca gaacacgtaa atccgaagaa agaccggaaa tacctaataa tcccgactct
    16441 ttattcacta cgcgttccag gtcacccagg ctctggttgg tttggctggc gacccaggac
    16501 atcgcaccaa agtcgacatc gccactgcgg gtacccatca tcaagccttc cagcggcgtc
    16561 attcccattg aagtatcaac actctgaccg ttgcgaaccg cgcagattga cgcgccattg
    16621 ccaagatgcg ccacaaccaa gccggagtca tcttccgcca gattcagcag cgaatgtgcg
    16681 cgctgggaaa catagcggtg cgacgtgccg tggaaaccat aacggcgtac acctaactct
    16741 tcataatatt tccacggcag gccgtataaa taagcttccg gagccatcgt ctggtggaaa
    16801 ctggtatcaa ataccgccac ctgagttacg cccggaaata attgctgcgc cgattcaata
    16861 ccacttaaat tggcgtaatt atgcaggggt gccagtggag aaacgcgacg gatattatca
    16921 atgacttcat cggtaataat ggcggactcg gtaaaaatac tgccgccgtg agcgatgcgg
    16981 tggccaatta aggccacact gtcatttaaa ttccgttttt ccagttcaaa tgcaattgcc
    17041 ttcaatgcac cttcgtagct gtggtgagcc agcggtgctg gctctccccc atttacggat
    17101 aagaatgcat tttccgagtt aataccgtcg gcaatacctg acattaatac ttcacagtcg
    17161 ctggcatcga gtacggaaaa cttaatcgaa gacgaaccac agttaataac caaaacaacc
    17221 ggaaattcat tcatctcttt tctcatcctg agttacggat taaaacagtt tgtatacgat
    17281 gttcaggatg gtcagcagac caatcacggt aacaaacacg ttatccagac gaccacggta
    17341 tttcgccaga gacggcgctt tacggatggc atacatcggc aacaggcaca gcagggatgc
    17401 gataatcggt gcgcccatgg cttcaataag gtcgaggatg ttcgggttgg cgtaggcaac
    17461 aacccaggtg gagcccatga tgaagatcat gctgatagtg ttcagtttac ccagcgacac
    17521 tttagttttg tcgcctttat aaccgaactt cagaatcaga ccattcaagc cttccagcgt
    17581 ccccagatag tgaccgaaga aagatttgaa gatagccacg agtgcgatga tggaagccgc
    17641 atattccagt gtaatcgcga acgttgtttt ggtaccggtc atggacgcaa agtggttagc
    17701 cagataagaa agcactggaa tattctgcgc tttggcttcc gccatgttgg ccggagacag
    17761 cgtaaacagg cagctaaagg caaagaacat caccactgca accatcagca tgctggcacg
    17821 agaaatgatt tgggaacatt tacgttcggt gaagtcgcga ccgaagtctt tctcatactc
    17881 ttcacgttta gaaaccacga aggaagagac gattggcgag aagttaaagg agaaaaccat
    17941 gatggaaatc cccagccaga cagtgatcag gataccgtca tgaccggtta acgacagcga
    18001 accgaggtca acctggtcga taactgcaga gttccagtaa gggatcagcg acagagaaat
    18061 cagcaccagg ctggcgataa acggccatac caggtagctc atcactttaa ccatcagatc
    18121 cttaccaaac cagatgacga aagccatcag cagcaacagg aacagcgcca caaagccgcg
    18181 attcagcggt gcaaatccga gctggttttc ccagaacgtc ataaaggtat tggtaatagt
    18241 aacgccataa atccacagca gtgggcaaat cgcgaagaag tacaggaacg tgataaccac
    18301 gccgccagtt ttaccaaaat gctcttccac cgtttccgta atgttgccgg aagggttaga
    18361 gccagaaaga cacagacgcg ccagcgcccg gtggcaataa aatgcgatgg ggtatgccaa
    18421 taccaacatc agaagaatcg ggatcagtcc gccaaaacct gcgcggatag ggaagaacag
    18481 caccccggcg ccgattgccg taccaaacaa gcctaacgtc catgtggtat ctgatttacg
    18541 ccaggacgat tgttttgtct ggctggatac aatgctatct gaagtactca tatcctatcc
    18601 tcaacgaatt aattaagcgt caacgaaacc ggtgatttga gagacgcgag aaagatcgat
    18661 attgccgccg gaaataatac tgacggtttt tctgttttga atatattggt ctaatttacc
    18721 gcttaataat gcagcacatg ccagagcgcc tgcgccttcg gtgacgactt tattgcgctg
    18781 aattaaggca atcatactgt ttctgatttc gtcttcgctg accagcacga tgtcatcgac
    18841 taattcacga acgatttcgt aagttaaatt acccgggcgg gagacatcac aaccatccgc
    18901 cagggtgccg gtagttcggt gcgtggttat ttctccggag tggaaagaag ccgccatgcc
    18961 gtgaacgttt tctgactgta cgccaataac acgaatggtc gggttaatag atttaattgc
    19021 caccgcaata ccagcaatta aaccaccacc accaattggc acaatcacgt tatcgacatc
    19081 atagagatct tccataattt ccagaccaat cgttccctgg ccagcaatca ctttcggatc
    19141 atcgtaaggt gggataaaaa tacgaccttc catttcgaca atttcgctca ctttagcgat
    19201 agtgtcgttg aaattatcac catgcagaac gacttctgcg gagtagtcgc acgttgccgc
    19261 tactttggat tttggcgcac cttttggcat caccacttta ccgtcgatac ccagcatcgc
    19321 gcaggagagc gaaacccctt gcgcatggtt gcccgcagaa caggccacta cacctttacg
    19381 tttttccgca tcagtcagtg aacttaattt attaaatgcg ccacgaattt taaatgaacc
    19441 cgtacgctgc atattttcaa acttcaggaa tatttcacct ttgcaacgtt cactaaaata
    19501 gtttgagcga ggcatgcctg ttttataaat tcgcccagcc agtcgttgtt tcgcttcaat
    19561 aatgtcatca atagcaaccg gcagatcgta tgtaatatgc attataaaac ctcttcgcct
    19621 gaattaaata tgtaggtaac cgtaaccgac acctgcaaaa cagacaggtg gattatttat
    19681 gtttaaataa aaacaaataa ctaaccaact tctattaatt gccttcgtct acacccatta
    19741 taagatgaat actctttggc taattccacc aaaaccgatg ctgctttttt aatacgataa
    19801 tttttcgacc ataccgcggc atattgtgcc acaggtaatg tttcttcaac cggaatagta
    19861 ataaattgat ttgaaccaaa aggtgacgtc atatcacaag gaattacagt taagaaatca
    19921 gcattgagaa caagattata aattgtcacg actgagtcgg ttttaacgat gttttcaata
    19981 ctgatgccat ttctttgtaa cgtagtaaga agctcgctgt agtaccccat attggtttgt
    20041 ggcaacaccc actgttcgtt cttcaacgac tccagcgtgg tggtgccggt gcatgttcgg
    20101 gacttactgg ctaccaacac aaactcggac tcgaacagcg gctcaacatg taaatcctga
    20161 agcttcattt ctgcacttaa cgtaccaatc gcaaaatcca gtcgaccgtc gcggattgcc
    20221 ggtaagaacg aagacagttg cgcttcatac atagaaacct gcgctttcgg gaacacctct
    20281 ttgaacttgt tgatcatccc tgacataaaa gtaaaaccaa tcaatgaagg aaaaccaaat
    20341 gagacttcca ccaccgcctc agaagacata ccgcttatct cattaaccat atttttcatt
    20401 tcacgggtaa tggattcgga acgggagagt aacaattgac cggcaggtgt taatgttaca
    20461 ccggtatttt tccgcaccac taattccaca ccaaaataat cttcaatatc gttaatgatt
    20521 ttactgacgg ctggttgagt taaccctaat tcttttgcag ccgagccgat agagccactt
    20581 ctaatgactt cctgaaagac taccaggtgc tgcgttttcg gaagaagaat agtgctcata
    20641 acgacctacg ttaattacct cattgacggc atgaagtgta tcaaaatgaa atgaacagga
    20701 tatgtacgac cactcacaaa tt
//
LOCUS       DAZFQD010000091        20710 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.091, whole genome shotgun sequence.
ACCESSION   DAZFQD010000091 DAZFQD010000000
VERSION     DAZFQD010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20710
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.091"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            201..1244
                     /locus_tag="ACHKTW_003314"
     CDS             201..1244
                     /locus_tag="ACHKTW_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFO6464280.1"
                     /translation="MNDRLCFEVHDNKGYFVFPDTWFGPLLGEFEEVLDAYDADEISE
                     TSYINKLRRLAQREPDFIDIHAHLAYAFLEQNAPRKALNAALKGLAAGNRIIPESFCG
                     EIIWMHPENRPYLRALYAAILANVHLQRHQDAVMLTDKILAYNPEDNQGARWLLGSEL
                     LRTGDHERAFSVLKEHADEFSPYWYELGLLHFLNGEHVKAATAFRHGFATNTYIAEML
                     CGNLHPFPLAVWHDFSGSLDTAEDYYATYSPLWGQYPEALLFVNWLYNHSSVLHERAE
                     IIKCAEMLIQEDDFEICESILRQQEHLWKRIDKTLSEEIVQKCRNMNGEYIWPWILPF
                     SAVGMKHSSIQHQ"
     gene            1759..5274
                     /locus_tag="ACHKTW_003315"
     CDS             1759..5274
                     /locus_tag="ACHKTW_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HFO6464281.1"
                     /translation="MDENALGFASYWRNSLADAESGKGSFERKDAKNFTHWHGIAAGR
                     LDEAIVSKFFKGEKDDVETVDVILRPKVYFRLLQHGKDRSAGAPDIVTPIVTPALLSR
                     EGFLYPTPATSIPRDLLEPLPKGAFSIGEIGQYDKYKTTHTTFSINFDDSVDKTAETD
                     EEREARYAALQQEWRQYLYDSERLLKSVAGDWIEKPEQYELAEHGYIVKTAQSGGASF
                     HILSLYDHLLVCNKDVPLFNRFASREVHAAESLLAPGAKFSDRLGHSGDKFPLAKAQR
                     DALSHFLDARHGDILAVNGPPGTGKTTLVLSIIATQWARAALEKSEPPVIIATSTNNQ
                     AVTNIIEAFGKDFSQGSGAMAGRWLPELKSFGAYFPSSSRKAEAAKKYQTEDFFNQVE
                     SKEYVEDALLFYLEKAKAAFPGKECSSPEKVIELLHGQLAAKSEQLIRLNATWQTLSQ
                     IRAARELIANDIEQYLNNLNKLLSGQEQKVTLLKSAKTEWKKYRAGESLIYSLFSWLP
                     AVRNKRQYQIQLFLEDKLGALIAGNQWSDPETIERNIDGLLNSAEREQTTYRQQIDSA
                     HEIVLKEQQAVQEWQRLAFDLGYEGDEELSFSQADELADTQIRFPAFLLTTHYWEGRW
                     LMDMARIDDLQDEKKKKGAKGVTARWQRRMKLTPCVVMTCYMLPGNMQISEHKGQRKF
                     EKSYLYDFADLLIVDEAGQVLPEVAAASFALAKKALVIGDTEQIPPIWSIAPAIDVGN
                     MLEEKILSGSTQEEITEKYTAIADLGKSAASGSVMKIAQFASRYQYDPELARGMYLYE
                     HRRCYDNIIGYCNTLCYHGKLLPKRGREKSNLMPAMGYLHIDGKGELASSGSRYNLLE
                     AETIAAWLAENQQNIEAHYGKSLHEVIGIVTPFSAQVSTIKQALGKQGISTGANEKSL
                     TVGTVHSLQGAERAIVVFSPVYSKHEDGGFIDSDNSMLNVAVSRAKDSFLVFGDMDLF
                     EVQPPSSPRGLLAKYLFESEKNALSFDYKERKDLKTSGTKIYTLHGVEQHDKFLNQTF
                     ENTSKYITIVSPWLTWQKLEQTGFLDSMIAACSRGINVTIVTDRSYNTEHKDYEKRKE
                     KQQNLKGTLEKLNAFGITTKLVNRVHSKIVIGDDGLLCVGSFNWFSATREARYERYDT
                     SMVYCGDNLKGEIEAIYNSLEKRQV"
     gene            complement(5383..6882)
                     /locus_tag="ACHKTW_003316"
     CDS             complement(5383..6882)
                     /locus_tag="ACHKTW_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001535067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HFO6464282.1"
                     /translation="MNIRKLFCPGNTPRILLFLFFFVVSAITSIACGYTEKNATGNVL
                     LLFLLLLLAHRNTLTSITALLFLFCCALYAPAGMTYGKINNSFIVALLQTTADEAAEF
                     TGMIPVYHFLVSAAILVFIVIFWRAHHRGHRNWLALLLFVLCSVNSWPLRMVKGTVVG
                     ATDTLREMQRYKQLSQHGADNWKILPGVPLYDTIVIVTGESVRRDYMSVYGYPVPTTP
                     WLNTAPGLFIDGYTSTAASTVPSLSRTLIYDYEQNPDSGNNVVALAAKAGYSTWWISN
                     QGKLGEHDTRISVIASDAEHTVFLKKGSFASRKTDDMLLLQETERALADKSSPKVIFL
                     HMIGSHPNPCDRLNSWPNHYLEQYPRKIACYLASISKLDNFLGQLDGILRRHSRHFAM
                     LYFSDHGLSVSDSANPVHHDGHVQGGYSVPLINTASDITSHQSVSRKISARHFTGIFQ
                     WLTGIRTENIPPFNPLTDEDNEPVMVFNGEKNVPADSLKPQPLILPDRR"
     gene            complement(7038..7778)
                     /locus_tag="ACHKTW_003317"
     CDS             complement(7038..7778)
                     /locus_tag="ACHKTW_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001615471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HFO6464283.1"
                     /translation="MKKVIVVSVFAMAGMFSAQALADESKTGFYMTGKAGTSVLSLSN
                     QRLIDGEGEWADKYKGGNDHDTVFSGGIAAGYDFYPQFSIPVRTELEFYARGKADSKY
                     NVDKDSWSGGYWRDDLKNEVSVNTLMLNTYYDFRNDSAFTPWISAGIGYARVHQKTTG
                     ISIWDHGYGNSGRESLSRSGSADNFAWSLGAGVRYDVTPDIALDLSYRYLDAGDASVS
                     YKDEWGDKYKSEVDVKSHDIMLGMTYNF"
     gene            8204..10030
                     /gene="atoS"
                     /locus_tag="ACHKTW_003318"
     CDS             8204..10030
                     /gene="atoS"
                     /locus_tag="ACHKTW_003318"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     AtoS"
                     /protein_id="HFO6464284.1"
                     /translation="MNYIKWIYPRRLRNQMILMAILMVIVPTLAIGYIVETEGRSAVL
                     SEKEKKLSAVVNLLNQALGDRYDLYIDLPREERIRALNAELAPITENITHAFPGIGAG
                     YYNKTLDAIITYAPSALYQNNVGVTIAADHPGREVMRTNTPLVYSGRQVRGDILNSMI
                     PIERNGEILGYIWANELTEDIRRQAWKMDVRIIIVLTAGLLISLLLIVLFSRRLSANI
                     DIITDGLSTLAQNIPTRLPQLPGEMGQISQSVNNLAQALRETRTLNDLIIENAADGVI
                     AIDRQGDVTTMNPAAEVITGYQRQELVGQPYSMLFDNTQFYSPVLDTLEHGTEHVALE
                     ISFPGRDRTIELSVTTSRIHNTHGEMIGALVIFSDLTARKETQRRMAQAERLATLGEL
                     MAGVAHEVRNPLTAIRGYVQILRQQTSDLIHQEYLSVVLKEIDSINKVIQQLLEFSRP
                     RHSQWQQVSLNALVEETLVLVQTAGVQARIDFISELDNELSPINADRELLKQVLLNIL
                     INAVQAISARGKIRIRTWQYSDSQQAISIEDNGCGIDLSLQKKIFDPFFTTKASGTGL
                     GLALSQRIINAHQGDIRVASLPGYGATFTLILPINPQGNQTV"
     gene            10027..11412
                     /gene="atoC"
                     /locus_tag="ACHKTW_003319"
     CDS             10027..11412
                     /gene="atoC"
                     /locus_tag="ACHKTW_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate metabolism transcriptional
                     regulator AtoC"
                     /protein_id="HFO6464285.1"
                     /translation="MTAINRILIVDDEDNVRRMLSTAFALQGFETHCANNGRTALHLF
                     ADIHPDVVLMDIRMPEMDGIKALKEMRSHETRPPVILMTAYAEVETAVEALRCGAFDY
                     VIKPFDLDELNLIVQRALQLQSMKKESRHLHQALSTSWQWGHILTNSPAMMDICKDTA
                     KIALSQASVLISGESGTGKELIARAIHYNSRRAKGPFIKVNCAALPESLLESELFGHE
                     KGAFTGAQTLRQGLFERANEGTLLLDEIGEMPLVLQAKLLRILQEREFERIGGHQTIK
                     VDIRIIAATNRDLQAMVKEGTFREDLFYRLNVIHLILPPLRDRREDISLLANHFLQKF
                     SSENQRDIIDIDPMAMSLLTAWSWPGNIRELSNVIERAVVMNSGPIIFSEDLPPQIRQ
                     PVCNAGEVKTSPVGERNLKEEIKRVEKRIIMEVLEQQEGNRTRTALMLGISRRALMYK
                     LQEYGIDPADV"
     gene            11608..12270
                     /gene="atoD"
                     /locus_tag="ACHKTW_003320"
     CDS             11608..12270
                     /gene="atoD"
                     /locus_tag="ACHKTW_003320"
                     /EC_number="2.8.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate CoA-transferase subunit alpha"
                     /protein_id="HFO6464286.1"
                     /translation="MKTKLMTLQDATGFFRDGMTIMVGGFMGIGTPSRLVEALLESGV
                     RDLTLIANDTAFVDTGIGPLIVNGRVRKVIASHIGTNPETGRRMISGEMDVVLVPQGT
                     LIEQIRCGGAGLGGFLTPTGVGTVVEEGKQTLTLDGKTWLLERPLRADLALIRAHRCD
                     TLGNLTYQLSARNFNPLIALAADITLVEPDELVETGELQPDHIVTPGAVIDHIIVSQE
                     SK"
     gene            12270..12920
                     /gene="atoA"
                     /locus_tag="ACHKTW_003321"
     CDS             12270..12920
                     /gene="atoA"
                     /locus_tag="ACHKTW_003321"
                     /EC_number="2.8.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate CoA-transferase subunit beta"
                     /protein_id="HFO6464287.1"
                     /translation="MDAKQRIARRVAQELRDGDIVNLGIGLPTMVANYLPEGIHITLQ
                     SENGFLGLGPVTTAHPDLVNAGGQPCGILPGAAMFDSAMSFALIRGGHIDACVLGGLQ
                     VDEEANLANWVVPGKMVPGMGGAMDLVTGARKVIIAMEHCAKDGSAKILRRCTMPLTA
                     QHAVHMLVTELAVFRFIDGKMWLTEIADGCDLATVRAKTEARFEVAADLNTQRGDL"
     gene            12917..14239
                     /gene="atoE"
                     /locus_tag="ACHKTW_003322"
     CDS             12917..14239
                     /gene="atoE"
                     /locus_tag="ACHKTW_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00366 family protein"
                     /protein_id="HFO6464288.1"
                     /translation="MIGRISRFMTRFVSRWLPDPLIFAMLLTLLTFVIALWLTPQTPI
                     SMVKMWGDGFWNLLAFGMQMALIIVTGHALASSAPVKSLLRTAASAAKTPVQGVMLVT
                     FFGSVACVINWGFGLVVGAMFAREVARRVPGSDYPLLIACAYIGFLTWGGGFSGSMPL
                     LAATPGNPVEHIAGLIPVGDTLFSGFNIFITVALIVVMPFITRMMMPKPSDVVSIDPK
                     LLMEEADFQKQLQKDAPPSERLEESRILTLIIGALGIAYLAMYFSEHGFNITINTVNL
                     MFMIAGLLLHKTPMAYMRAISAAARSTAGILVQFPFYAGIQLMMEHSGLGGLITEFFI
                     NVANKDTFPVMTFFSSALINFAVPSGGGHWVIQGPFVIPAAQALGADLGKSVMAIAYG
                     EQWMNMAQPFWALPALAIAGLGVRDIMGYCITALLFSGVIFVIGLTLF"
     gene            14270..15454
                     /gene="atoB"
                     /locus_tag="ACHKTW_003323"
     CDS             14270..15454
                     /gene="atoB"
                     /locus_tag="ACHKTW_003323"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HFO6464289.1"
                     /translation="MKNCVIVSAVRTAIGSFNGSLASTSAIDLGATVIKAAIERAKID
                     SLHIDEVIMGNVLQAGLGQNPARQALLKSGLAETVCGFTVNKVCGSGLKSVALAAQAI
                     QAGQAQSIVAGGMENMSLAPYLLDAKARSGYRLGDGQVYDVILRDGLMCATHGYHMGI
                     TAENVAKEYGITREMQDELALHSQRKAAAAIESGAFTAEIVPVNVVTRKKTFVFSQDE
                     FPKADSTAEALGALRPAFDKAGTVTAGNASGINDGAAALVIMDESAALAAGLTPLARI
                     KSYASGGVPPALMGMGPVPATQKALQLAGLQLADIDLIEANEAFAAQFLAVGKTLGFD
                     PEKVNVNGGAIALGHPIGASGARILVTLLHAMQARDKTLGLATLCIGGGQGIAMVIER
                     LN"
     gene            complement(15864..16049)
                     /locus_tag="ACHKTW_003324"
                     /pseudo
     CDS             complement(15864..16049)
                     /locus_tag="ACHKTW_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_063945310.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            16110..17243
                     /locus_tag="ACHKTW_003325"
     CDS             16110..17243
                     /locus_tag="ACHKTW_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000555401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISEc45 family transposase"
                     /protein_id="HFO6464290.1"
                     /translation="MHSENIAAYIGLDVHKETLAVAIAAPERLGEVRYYGTINNEAQA
                     VRRLFQKLQGLYGNILSCYEAGPCGFGLYHQLTAMNIKCQVIAPSRIPKSPTDRIKND
                     HRDAISLARLLRAGELTPVWIPDLTHEAMRDLIRARAAAKRDSRVARQRILSMLLRTD
                     KRYAGKHWTGKHRTWLANQSFSQPSQQIAFQHYCQSLEQIEDRILQLDQEISRLLPEW
                     SLCNLVCQLQALKGVGQLTAITLVAELGDFSRFSSPKQLMAFLGLVPGEYSSGNSIRP
                     RGITKVGNSELRRLLYEAAWSYRTPAKVGAWLIYYRPDSVTQYSKDIAWKAQQRLCSR
                     YRSLTAKGKKSQVAITAVARELTGFMWDIALAAQSSFSQQKQN"
     gene            complement(17496..17966)
                     /locus_tag="ACHKTW_003326"
                     /pseudo
     CDS             complement(17496..17966)
                     /locus_tag="ACHKTW_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            18234..18473
                     /locus_tag="ACHKTW_003327"
                     /pseudo
     CDS             18234..18473
                     /locus_tag="ACHKTW_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001088287.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            18771..18959
                     /locus_tag="ACHKTW_003328"
     CDS             18771..18959
                     /locus_tag="ACHKTW_003328"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6464291.1"
                     /translation="MTNEELQAIALSMLERQREKVHQQRESLLNPSPFTKPKRLELEA
                     FLDKYPRRLKSGKTRPPG"
     gene            19287..19481
                     /locus_tag="ACHKTW_003329"
     CDS             19287..19481
                     /locus_tag="ACHKTW_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6464292.1"
                     /translation="MNENRIKRLEQLLQARQREFATKWGRAALPHERLIYFPLEDLIK
                     NNGKKIRPDKKHFLECNEDK"
     gene            complement(20280..20604)
                     /locus_tag="ACHKTW_003330"
                     /pseudo
     CDS             complement(20280..20604)
                     /locus_tag="ACHKTW_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502602.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         5665 a         4893 c         4914 g         5238 t
ORIGIN      
        1 cagatataat ttagaatttc aatcatctga catcattata aaaaatgaga ttcacataaa
       61 gataaaatta ttgttctcaa ggaaagcatg aaggggcttc gtaagcaata ttcattcggt
      121 gccaggacag gacttgatac acagaatctc aacttttatg ctctcagact atatcaccac
      181 atgttttaca ggctcagaaa atgaacgata gattatgctt tgaagtgcac gataacaagg
      241 gttatttcgt atttccggat acctggtttg gcccattgct gggggagttt gaagaagtgc
      301 ttgatgcata cgacgctgat gaaatcagcg agacgagtta catcaacaaa ctccgacgcc
      361 ttgctcaacg ggagccggac tttattgata tacacgctca tctggcatat gcatttctgg
      421 aacagaatgc cccgcgtaag gcactgaacg ccgctcttaa aggactggcg gcaggtaatc
      481 gtatcatccc ggaaagtttc tgtggtgaaa taatctggat gcatcccgaa aaccggcctt
      541 atctgcgcgc gctttatgca gcaattctcg caaatgtgca cttacagcgc catcaggatg
      601 cggttatgct cactgataaa atactggcat acaatcctga agataatcag ggggctcgct
      661 ggttgttggg ttcagagctg ttacgcaccg gcgatcatga gcgagccttc agtgtactga
      721 aagagcatgc tgatgaattt tcaccttact ggtatgaact cggtctcctg cattttctga
      781 acggggaaca tgttaaggcc gccacagctt ttcgccatgg ctttgccaca aacacttaca
      841 tcgcagaaat gctgtgcgga aatctgcatc catttccact tgctgtgtgg catgacttct
      901 ccggctctct ggatacggca gaagattact atgcaacata ctcaccatta tggggacaat
      961 atccggaagc attgctgttt gtaaactggc tctataacca ttcctccgtg ctgcatgaac
     1021 gagcagaaat cattaaatgc gccgaaatgc tgattcagga agatgatttc gaaatatgcg
     1081 aaagtatttt aagacagcag gagcatctgt ggaaaagaat tgacaagaca ctttctgagg
     1141 aaattgtaca gaaatgcaga aatatgaacg gtgaatatat ctggccctgg atattgccct
     1201 tttccgcagt agggatgaaa cattcaagca tccagcatca gtagatattg cattagtgta
     1261 actttaaaac aagtaataat ttattatcca ataataacac tgtgttcctt ccccaggtga
     1321 ttaccctggg gttattttct gaaagtttcc tttgcaaaag gatagcgttg taaattactt
     1381 taacgaatta ctccatgaac tcattaaagt tctttcgtaa tactttatcg tcaaacacgt
     1441 tatagctggc atcaaaaacc acagatttac cctgccagcg ggtaatcccc tcctctggcg
     1501 ttttcggttg aactggtatc cccgtccaac aaaggcagcc attataaaca gtaaaaaatt
     1561 gtattcgctg ttatgaaatc aatagtcctg acgtactgtt gaatgtgctt gtgttctgaa
     1621 cctcgtacaa ccagtacatt tgatatcgaa ttataaacaa aacattgcat ccaacttttt
     1681 caatatggac aatagttgtc catacggtgg ttttatattc gataaatcaa tggatttcat
     1741 tgagtcacag gactggtaat ggatgaaaat gctttagggt ttgcctcata ctggcgcaac
     1801 tcgcttgcgg atgctgagtc aggaaagggc agttttgaac ggaaagacgc caaaaatttc
     1861 actcactggc atgggatagc ggcgggacgt cttgacgaag cgattgtcag taaatttttt
     1921 aagggagaaa aagacgatgt agaaacggtc gatgtcatct tgcgcccaaa agtttatttt
     1981 cggttactgc agcatggtaa ggaccgttct gcaggtgcgc ctgatattgt taccccgata
     2041 gtgacgccag ccttgctaag ccgtgagggt tttttatatc cgacgccagc gacctccatt
     2101 cccagagacc tgcttgaacc tttgccaaaa ggagcatttt cgattggtga gattgggcag
     2161 tatgacaaat acaagacgac ccataccacg ttctctatca actttgatga cagcgttgat
     2221 aagactgccg aaacggatga agaacgggaa gcacgatatg ccgccttgca gcaggagtgg
     2281 cgtcaatatc tgtatgactc agagaggcta ctgaagagcg ttgccggcga ctggattgaa
     2341 aaacctgagc aatatgaact cgctgagcac ggttatattg ttaaaacggc tcaatctggc
     2401 ggtgccagtt tccatatcct ttctctttat gatcacctgc ttgtttgcaa taaggatgtg
     2461 ccgctcttca atcgattcgc ctcgcgagag gttcatgctg cagagtcttt gctggcccct
     2521 ggagcaaaat tcagcgacag gcttggacac tccggagata agtttccgct ggcaaaggct
     2581 cagcgcgatg ccttaagcca ttttctggat gccagacatg gcgatatcct tgctgttaat
     2641 ggccctccgg gaaccggaaa aaccacgctg gtgctttcta tcatcgccac gcagtgggcc
     2701 agagcggctc tcgaaaaatc tgagcctccg gttattatcg cgacttcaac gaataaccag
     2761 gctgtaacga acattattga ggcattcggg aaagactttt cgcaaggttc aggtgcgatg
     2821 gccgggcgat ggttgccaga gctgaaaagc ttcggcgctt attttccctc aagcagtcgt
     2881 aaagctgagg cagccaaaaa atatcaaact gaagatttct tcaaccaggt tgagtcaaaa
     2941 gagtatgtag aggatgcact gctgttttat ctggaaaagg ctaaggcagc ctttcctgga
     3001 aaagagtgtt catcccctga aaaggtcatt gaactcctgc atggtcagtt ggcagcaaaa
     3061 tctgagcaac tgataagact gaacgcaaca tggcaaacgt taagccagat tcgggctgcg
     3121 cgtgagctta ttgctaatga tattgagcaa tatctcaata atttaaataa attactttcc
     3181 ggacaagaac aaaaagtcac tctactgaag agtgctaaaa cggaatggaa aaaatatcgc
     3241 gccggtgaat cactgatcta ttcattattt tcctggctcc cggcggttcg caataagcga
     3301 cagtaccaaa tacagctgtt tctcgaagat aaattaggcg cgctgattgc aggaaatcag
     3361 tggtctgatc ctgaaactat cgaacgtaat attgatgggc tgctcaattc cgctgagcgc
     3421 gagcaaacaa cataccggca gcagattgac tccgcccatg aaatcgttct taaagaacag
     3481 caggcggttc aggagtggca gaggctggca tttgatttag ggtatgaggg cgacgaggaa
     3541 ctgagcttct cacaggccga tgaactggct gatacgcaga ttcgcttccc tgcattttta
     3601 ctgacgactc actactggga aggtcgttgg ctgatggata tggccagaat tgatgatctg
     3661 caggacgaga agaagaaaaa aggtgctaaa ggggtaaccg cccgttggca acgtcgaatg
     3721 aaactcacgc catgtgtggt gatgacatgc tatatgctgc ccggcaatat gcagataagt
     3781 gagcacaaag gacaacgtaa attcgagaaa agttatttgt atgattttgc cgatttactc
     3841 attgtcgatg aagccgggca ggtgcttcct gaagtggctg ctgcctcgtt tgcattagct
     3901 aagaaggcat tagtgattgg cgatacggag cagatcccgc caatatggag tattgctccc
     3961 gcgattgatg tcggtaacat gctggaggaa aaaattctgt cgggcagtac gcaagaagag
     4021 attaccgaga aatatacggc aatcgcagac cttggtaaaa gtgccgcatc tggcagcgtt
     4081 atgaaaatag cgcagtttgc ttcgcgctat caatatgatc ccgaactggc tcgtggtatg
     4141 tacctatatg aacaccgccg gtgctacgac aatattattg gatactgtaa tacgctctgc
     4201 tatcacggta agttgttgcc taaaagaggg cgtgaaaaga gcaatttaat gcccgcaatg
     4261 gggtatctcc atattgatgg taaaggagag ctggcaagta gtggaagtcg atataatttg
     4321 cttgaggctg aaacaatagc ggcctggttg gcagagaacc agcaaaatat tgaagcgcat
     4381 tacggcaaat cgcttcatga agttatcggt atcgtgacgc cttttagcgc gcaggtatcc
     4441 actatcaaac aggcgctggg taaacaaggt atcagtactg gcgcgaatga aaagtcgctc
     4501 acagtgggca ccgtgcactc tcttcaggga gcggaaagag cgattgtggt attctcgcca
     4561 gtctattcaa aacatgaaga cggcgggttt attgatagcg ataacagcat gctgaatgtt
     4621 gcagtctccc gtgcgaagga tagcttcctg gtcttcggcg atatggatct gtttgaggtc
     4681 cagccgccct catctccgcg aggattgctg gcaaaatacc tctttgagtc agagaagaat
     4741 gcgctctctt ttgattataa agagcgtaag gatttaaaaa cctccgggac caaaatctac
     4801 acacttcatg gtgtggagca acatgataaa ttcctgaatc agacatttga aaataccagt
     4861 aaatacatca cgatagtttc tccatggctg acctggcaaa agctggagca aaccggtttt
     4921 cttgattcca tgattgcggc gtgttcacgt ggtattaacg tcacgatagt cactgacaga
     4981 agctacaaca ctgaacataa agattatgag aaacgaaaag agaagcagca gaatcttaaa
     5041 gggacgctgg aaaaactgaa tgcttttggt attactacaa agctggtaaa tcgcgttcat
     5101 agcaaaattg ttattggtga tgatggtttg ctgtgcgtgg gatcgttcaa ctggtttagc
     5161 gcaacgcgag aagcgcgata tgaacgatac gatacctcaa tggtttattg cggtgataac
     5221 ctgaagggtg agattgaggc tatttataat agtcttgaga agcgtcaggt ttagagcagg
     5281 aatggtgagt agccatctta tcgatcgttt tcgagcgtaa gatggctgaa tggaatagct
     5341 attattgcac agtccttaat tataacattc ataccaacat gattatcttc tgtccggaag
     5401 aatcaaaggc tgcggtttca gactgtctgc cggcacattt ttctctccgt taaaaaccat
     5461 aacgggttca ttatcttcgt ctgtcagcgg attgaatggc ggtatatttt cggtacgaat
     5521 accggtcagc cactgaaaaa tgcctgtgaa atgacgggca ctgatttttc tgctgacgga
     5581 ctgatgagac gtgatgtcac tggcggtatt aatcagggga acgctgtagc ctccctgcac
     5641 atgaccatca tgatgaacag gattagcact gtcgctgacc gacagcccat ggtcagaaaa
     5701 gtaaagcata gcgaaatgac gggaatgccg gcgaaggata ccatcaagct gaccgagaaa
     5761 gttatccagt ttactgatgc tggcgaggta acaggcaatc tttcggggat actgctccag
     5821 gtaatgattc ggccaggagt taagtcggtc acacggattc ggatgagagc caatcatgtg
     5881 caggaaaatc accttcggag aggatttatc cgccagcgca cgttctgttt cctgtaacaa
     5941 caacatgtca tccgttttac gggaagcaaa gctgcctttc ttgaggaaaa cggtatgctc
     6001 cgcatcagaa gcaataacag agatgcgtgt atcatgctcc cccagttttc cctgattgga
     6061 tatccaccat gtgctgtatc ctgcttttgc tgccagcgcc accacgttgt tgccggagtc
     6121 agggttctgc tcatagtcat aaatcagtgt ccggctcagg gaaggtacgg tactggctgc
     6181 tgtcgatgta tagccgtcaa taaataaacc gggagcagta ttcagccacg gcgtggttgg
     6241 cacgggatag ccatataccg acatataatc cctgcgcaca ctctcaccag taacgataac
     6301 aatcgtgtca tacaacggca cacccggcag gattttccag ttgtcagccc cgtgctgact
     6361 cagttgttta taacgctgca tttcacgcaa tgtgtcagtt gcccccacaa cagttccttt
     6421 aaccatccgc aacggccagc tgtttacaga gcataatacg aatagcagca gtgccagcca
     6481 gttacggtga ccgcggtggt gtgcccgcca gaaaatcact atgaatacca gaatcgcggc
     6541 actgaccaga aaatgataaa caggaatcat cccggtaaac tctgcagcct catcagccgt
     6601 ggtctgcagc aacgcgacaa taaaactgtt gttgatttta ccgtacgtca tacccgcagg
     6661 cgcatacagt gcacaacaga acagaaataa cagcgctgta atggatgtga gggtatttct
     6721 gtgtgcaagg agaagaagga gaaacagaag cagcacattt cctgtggcat tcttctcagt
     6781 gtatccgcat gcaattgagg ttattgcaga aacaacaaaa aagaataaaa acaataaaat
     6841 ccggggtgtg ttgcccggac aaaacaattt cctgatattc atcggagtat atcgacaaca
     6901 ttattatgaa gagaacaaga taataaaaat cagaaattat ctgtaaaaca gataacagac
     6961 aaccctgcag taatagactg cagggtattg ctgtttaatt gcagaagata tgcaattatc
     7021 gttcaggagc agtgtcgtca gaagttataa gtcataccaa gcatgatgtc atgactttta
     7081 acatcaactt ctgacttata tttatcgccc cactcgtcct tataactcac actggcatca
     7141 cctgcatcaa gatagcgata gctgaggtcc agcgcgatat ccggggtgac gtcatagcgg
     7201 acacctgcgc caaggctcca tgcaaagtta tcagcagagc ctgagcgtga caacgattcg
     7261 cgaccactgt ttccgtaccc atgatcccag atactgatac cggttgtttt ctggtgaact
     7321 ctggcgtagc caatccctgc agatatccat ggtgtgaatg cgctgtcatt ccggaagtca
     7381 tagtacgtat tcagcatcag tgtgttgact gacacttcat tcttcaggtc atcacgccag
     7441 taaccacctg accaactgtc tttatctacg ttatacttcg aatcagcttt tccacgagcg
     7501 taaaactcca gttccgtacg aaccggaata ctgaactgcg gataaaaatc ataaccagcc
     7561 gcgataccgc cactgaatac cgtatcatgg tcattaccac ctttatattt gtccgcccac
     7621 tctccttcac catcgataag acgctggttg gaaagagata aaacggaagt ccccgcctta
     7681 ccggtcatat aaaagcctgt tttgctctca tcagccaagg cctgggctga aaacataccc
     7741 gccattgcaa atactgaaac cacaataacc ttttttattt caatcatctc cgttccctac
     7801 ccgacaaaga ggctcaccta ttctaaaacc atgcaaaaga cagggtaaaa agagaaattt
     7861 cgatcgaatc gatcgacaaa atcgatcata catgatgaag atttcttatc gaatccataa
     7921 aaatagtgac aggtaacggg tactgcagga acaatcagaa atggaagttt gggaaagtgc
     7981 catagcacta cgtcgttgct aacatagagg aacagtgctt tgttgataag atcctttata
     8041 cgacgaatct attggatgaa aaataaacta cgtaatcacc tgattctcac tgatattgcc
     8101 aattctggta ggattgagtg attcttcgct tacaaaacga tgctcaggaa tatgataatt
     8161 gttatatatc gatgtataca ggacaatatt agtggatctc agcatgaatt atataaagtg
     8221 gatttaccca cgccgcttac gcaatcaaat gatcctgatg gcaatcctga tggtcattgt
     8281 cccaacgctt gctattggtt atatcgtaga aacggaagga cgttcagcag tcttatctga
     8341 aaaagagaaa aaactttctg ccgtggtcaa cctgcttaat caggcgctag gcgatcgcta
     8401 cgatctctac atcgacttac cgcgtgagga gcgtatccgc gcattaaatg cagaacttgc
     8461 ccccattacc gaaaatatca ctcacgcctt ccctggcatc ggcgctggtt attacaacaa
     8521 aacgctggat gcgataatca cctacgcgcc ttcagcgcta tatcagaata atgtgggcgt
     8581 taccattgcc gcagatcacc ctggtcgcga agtcatgcgt acaaataccc ctttggtgta
     8641 ttcaggcagg caggtgcgcg gcgatatttt gaattcaatg atccccattg agcgtaatgg
     8701 tgaaatcctc ggctatatct gggccaatga attaaccgaa gatattcgcc gccaggcctg
     8761 gaaaatggat gtgaggatta tcattgtgct caccgccggt ttgctgataa gcctgctgtt
     8821 gattgtcctt ttctcccgtc gcctgagcgc caatattgat atcatcaccg atggcctctc
     8881 gactctggca caaaatattc ccactcgatt accacaattg cccggtgaaa tggggcaaat
     8941 cagtcagagt gttaacaacc tcgcccaggc actgcgtgaa acgcggacac ttaacgatct
     9001 gattattgaa aacgctgccg atggcgtcat tgccattgac cgtcagggtg atgtaaccac
     9061 catgaacccg gcagcagaag tcatcactgg ctatcaacgt caggaactgg tagggcagcc
     9121 ttactccatg ttgttcgaca atactcagtt ctacagtcca gtactggata cgctggaaca
     9181 tggcaccgaa catgtagcgc tggagatcag ttttccaggc cgtgaccgca ccattgaact
     9241 cagtgtcact accagtcgaa ttcataacac gcatggtgaa atgataggtg ctttggtgat
     9301 tttctctgat ttaactgccc gcaaagaaac ccagcgccgc atggcgcaag cagaacgcct
     9361 cgccacactg ggtgagctga tggctggcgt cgcgcatgaa gtacgtaatc cgctaacggc
     9421 tattcgtggt tatgtacaga tcttgcgcca acaaaccagc gacctaatac atcaggaata
     9481 tctgtccgta gtactcaaag aaatcgattc aattaacaaa gttattcagc aattgctcga
     9541 attttcacgt ccacgccaca gtcaatggca acaagtcagc ctcaatgcat tggttgaaga
     9601 aactctggta ctggtacaaa ccgccggcgt acaagcgcgg atcgacttca taagcgaact
     9661 ggataatgaa ttaagcccga ttaacgccga tcgtgaactg ctcaaacagg tactactgaa
     9721 tatcctgatc aatgccgtcc aggctatcag tgcacgaggg aaaattcgca ttcgaacctg
     9781 gcagtacagc gactcacaac aggccatttc gatagaggac aacggctgtg gcattgatct
     9841 ctcgctgcaa aaaaagatct tcgatccctt tttcaccacc aaagcttcag gaaccgggct
     9901 tggtctggcg ttaagtcaac gcatcattaa tgcccatcag ggtgatattc gcgtcgccag
     9961 tttgccgggc tacggcgcaa ccttcaccct tattttaccg atcaatccgc agggaaatca
    10021 gactgtatga ctgctattaa tcgcatcctt attgtggatg atgaagataa tgttcgccgt
    10081 atgctgagca ccgcttttgc actacaagga ttcgaaacac attgtgcgaa caacggacgc
    10141 acagcattac acctgtttgc cgatattcac cctgacgtgg tgttgatgga tatccgcatg
    10201 ccagagatgg acggcatcaa ggcactcaag gagatgcgca gccatgagac ccggcccccc
    10261 gttattctga tgacggccta tgctgaagtg gaaaccgccg tcgaagcgct acgctgcggg
    10321 gccttcgact atgttattaa accgtttgat ctcgatgagt tgaatttaat cgttcagcgc
    10381 gctttacaac tccagtcaat gaaaaaagag agccgtcatc tgcaccaggc actgagcacc
    10441 agctggcaat gggggcacat tctcaccaac agcccggcga tgatggatat ctgcaaagac
    10501 accgccaaaa ttgccctttc tcaggccagc gtcttgatta gcggtgaaag cggcaccggg
    10561 aaagagttga ttgccagagc gattcactac aattcgcggc gggcaaaggg gccgttcatt
    10621 aaagtcaact gcgcggcgct gccggaatcg ttgctcgaaa gtgaactgtt tggtcatgaa
    10681 aaaggtgcat ttactggtgc acaaaccttg cgtcagggat tatttgaacg tgccaacgaa
    10741 ggtactctgc tcctcgacga aattggcgaa atgccgctgg tactgcaagc caaattacta
    10801 cgcattctgc aggaacggga atttgaacgg attggcggcc atcagaccat aaaagttgat
    10861 atccgcatca ttgctgccac caaccgtgac ttgcaggcaa tggtaaaaga aggcaccttc
    10921 cgtgaagatc tcttttatcg ccttaacgtt attcatttaa tactgccgcc tctgcgcgat
    10981 cgccgggaag atatttccct gttagctaat cactttttgc aaaaattcag tagtgagaat
    11041 cagcgcgata ttatcgacat cgatccgatg gcaatgtcac tgcttaccgc ctggtcatgg
    11101 ccgggaaata ttcgagagct ttccaacgtt attgaacgcg ccgtcgtgat gaactcaggc
    11161 ccgatcattt tttctgagga tcttccgccg cagattcgtc agccagtctg taatgctggc
    11221 gaggtaaaaa catcccctgt cggagagcgt aatttaaaag aggaaattaa acgcgtcgaa
    11281 aaacgcatca ttatggaagt gctggaacaa caagaaggaa accgaacccg cactgcttta
    11341 atgctgggca tcagtcgccg tgcattgatg tataaactcc aggaatacgg tatcgatccg
    11401 gcggatgtat aacgccaaaa cttgctatgc agaaaattgc acagtgcgca attttctgca
    11461 tagccgctca ttatccttat aaatccccat ccaatttatc ccttcatatt caattagtta
    11521 aataacaaaa tccagtaatc tcattctggc actccccttg ctattccctg actgtaccca
    11581 caacggtgta tgcaagaggg ataaaaaatg aaaacaaaat tgatgacatt acaagatgcc
    11641 accggcttct ttcgtgacgg catgaccatc atggtgggcg gatttatggg gattggcact
    11701 ccatcccgcc tggttgaagc attactggaa tctggtgttc gcgacctgac attgatagcc
    11761 aatgataccg cgtttgttga tactggcatc ggtccgctca tcgtcaatgg tcgagtccgc
    11821 aaagtgattg cttcacatat cggcaccaac ccggaaacag gtcggcgcat gatatctggt
    11881 gagatggacg tcgttctggt gccgcaaggt acgctaatcg agcaaattcg ctgtggtgga
    11941 gctggacttg gtggttttct caccccaacg ggtgtcggca ccgtcgtaga ggaaggcaaa
    12001 cagacactga cactcgacgg taaaacctgg ctgctcgaac gcccactgcg cgccgacctg
    12061 gcgctaattc gcgctcatcg ttgcgacaca cttggcaacc tgacctatca acttagcgcc
    12121 cgcaacttta accccctgat agcccttgcg gctgatatca cgctggtaga gccggatgaa
    12181 ctggtcgaaa ccggcgagct gcaacctgac catattgtca cccctggtgc cgttatcgac
    12241 cacatcatcg tttcacagga gagcaaataa tggatgcgaa acaacgtatt gcgcgccgtg
    12301 tggcgcaaga gcttcgtgat ggtgacatcg ttaacttagg gatcggttta cccacaatgg
    12361 tcgccaatta tttaccggag ggtattcata tcactctgca atcggaaaac ggcttcctcg
    12421 gtttaggccc ggtcacgaca gcgcatccag atctggtgaa cgctggcgga caaccgtgcg
    12481 gcattttacc cggcgcagcc atgtttgata gcgccatgtc atttgcgcta atccgtggcg
    12541 gtcatattga tgcctgcgtg ctcggcggtt tgcaggtaga cgaagaagcc aacctcgcga
    12601 actgggtagt gcctggaaaa atggtgcccg gtatgggtgg cgcgatggat ctggtgaccg
    12661 gggcacgcaa agtgatcatt gccatggaac attgcgccaa agatggttca gcaaaaattt
    12721 tgcgccgctg caccatgccg ctcactgcgc aacacgcggt gcatatgctg gtcactgaac
    12781 tggctgtctt tcgtttcatt gacggcaaaa tgtggctcac cgaaattgcc gacgggtgtg
    12841 atttagccac cgtgcgtgcc aaaaccgaag ctcggtttga agtcgccgcc gatctgaata
    12901 cgcaacgggg tgatttatga ttggtcgcat atcgcgtttt atgacgcgtt ttgtcagccg
    12961 gtggcttccc gatccactga tctttgccat gttgctgaca ttgctaactt tcgtgatcgc
    13021 gctttggtta acaccacaaa cgccgatcag catggtgaaa atgtggggtg acggtttctg
    13081 gaacttgctg gcgtttggta tgcagatggc gcttatcatc gttaccggtc atgcccttgc
    13141 cagctctgct ccggtaaaaa gtttgctgcg tactgccgcc tccgccgcaa agacgcccgt
    13201 acagggcgtc atgctggtta ctttcttcgg ttcagtcgct tgtgtcatca actggggatt
    13261 tggtttggtt gtcggcgcaa tgtttgcccg tgaagtcgcc cgccgagtac ccggttctga
    13321 ttatccgttg ctcattgcct gcgcctacat tggttttctc acctggggtg gcggtttctc
    13381 tggctcaatg cctctgttgg ctgcaacacc gggcaacccg gttgagcata tcgccgggct
    13441 gatcccggtg ggcgatactc tgttcagtgg ttttaacatt ttcatcactg tggcgttgat
    13501 tgtggtgatg ccatttatca cccgcatgat gatgccaaaa ccgtctgacg tggtgagtat
    13561 cgatccgaaa ctactcatgg aagaggctga ttttcaaaag cagctacaga aagatgcccc
    13621 accatccgag cgactggaag aaagccgcat tctgacgttg atcatcggcg cactcggtat
    13681 cgcttacctg gcgatgtact tcagcgaaca tggcttcaac atcaccatca ataccgtcaa
    13741 cctgatgttt atgattgcgg gtctgctgct acataaaacg ccaatggctt atatgcgtgc
    13801 tatcagcgcg gcagcacgca gtactgccgg tattctggtg caattcccct tctacgctgg
    13861 gatccaactg atgatggagc attccggtct gggcggactc attaccgaat tcttcatcaa
    13921 tgttgcgaac aaagacacct tcccggtaat gacctttttt agttctgcac tgattaactt
    13981 cgccgttccg tctggcggcg gtcactgggt tattcaggga cctttcgtga tacccgcagc
    14041 ccaggcgctg ggtgctgatc tcggtaaatc ggtaatggcg atcgcctacg gcgagcaatg
    14101 gatgaacatg gcacaaccat tctgggcgct gccagcactg gcaatcgccg gactcggtgt
    14161 ccgcgacatc atgggctatt gcatcactgc cctgctcttc tccggcgtca ttttcgtcat
    14221 tggtttaacg ctgttctgac ggcaacccta cagacagaag gaataaaaaa tgaaaaattg
    14281 tgtcatcgtc agtgcggtac gtactgctat cggtagtttt aacggttcac tcgcttccac
    14341 cagcgccatc gacctggggg cgacagtaat taaagccgcc attgaacgtg caaaaatcga
    14401 ttcactacac attgatgaag tgattatggg taacgtgttg caagccgggt tggggcaaaa
    14461 tccggcacgt caggcactgt taaaaagcgg gctggcagaa acggtgtgcg gattcacggt
    14521 caataaagtg tgtggttcgg gtcttaaaag tgtggcgctt gccgcccagg caattcaggc
    14581 aggtcaggcg caaagcattg tggcgggggg tatggaaaat atgagtttag cgccctactt
    14641 actcgatgca aaagcacgct ctggttatcg tcttggagac ggacaggttt atgacgtaat
    14701 cctgcgcgat ggtctgatgt gcgccaccca tggttatcat atggggatta ccgccgagaa
    14761 cgtggccaaa gagtacggaa ttacccgaga aatgcaggat gaactggcgc tacattcaca
    14821 gcgtaaagcg gcagccgcaa ttgagtccgg tgcttttaca gccgaaatcg tcccggtaaa
    14881 tgttgtcacc cggaagaaaa ccttcgtctt cagtcaagac gaattcccga aagcggattc
    14941 tacggctgaa gcgttaggcg cattgcgccc ggccttcgat aaagcaggaa cagtcaccgc
    15001 cgggaacgcg tctggcatta acgacggtgc tgccgctctg gtgattatgg atgaatctgc
    15061 ggcgctggca gcaggcctta cccccctggc tcgcattaaa agttatgcca gcggtggcgt
    15121 gccccccgca ttgatgggta tggggccagt acctgccacg caaaaagcgt tacaactggc
    15181 ggggctgcaa ctggcagaca ttgatctcat cgaagctaat gaagcatttg ctgcacagtt
    15241 ccttgctgta gggaaaaccc tgggctttga tcctgagaaa gtgaatgtca acggcggggc
    15301 catcgcgctc gggcatccta tcggtgccag tggtgcacgt attctggtca ctctgttaca
    15361 tgcaatgcag gcacgcgata aaaccctggg cctcgcaaca ctgtgtattg gtggcggtca
    15421 ggggattgcg atggtgatcg aacgattgaa ttaacagcag aagccacctc cagtccatag
    15481 gaggtggttt cacaggcctc ataaaaactc gtcagagtgt aacttgagtc cttactgaaa
    15541 caggattaga gcagaagatg ttcatttgta aatataattg gagagacgaa cagcaattag
    15601 agagagtcgc ctcctcccag ttatttgtat aattcataaa caccatctca aattcctcac
    15661 atcaaaatcc attacgttgc tggaaacagc aaaggttcag gcttatttat ccgtctctgt
    15721 taaaacatca gaaagcaacg taccgatggg caaaacgatt acattcattg tcaataactt
    15781 atatcatcca caaaaggccg cgaaacgttg cgctggttga aggaaaatcg taagcgtcaa
    15841 cagagcgccg tattgacact tatttatcgg tgataaacta cgttccatgg cagcagttcg
    15901 tcaacacggt tggagggcca ttccggcagt acgctcagga tatggcgcag atacgcttct
    15961 ggatcgatac cgttcaaccg acagctcccg attagtccgt acagcagagc tccacgctcg
    16021 cctccatgat cgttgccgaa gaacatgtag atggtatgac ctttatatac tcactatagc
    16081 attggtggga tattccagag gaggtcatta tgcattcgga aaatattgca gcttacatcg
    16141 ggctggatgt acataaagaa acgttggcgg ttgctattgc tgcacctgaa cggcttggcg
    16201 aagttcgtta ttatggtacg attaacaacg aagcccaggc tgtacgtcga ttatttcaga
    16261 aattgcaggg actttacgga aacattcttt cctgctatga ggctggcccc tgtggttttg
    16321 ggctatatca tcaactcaca gcgatgaata taaaatgcca ggttattgcc ccttcccgga
    16381 taccaaaatc accgacagac agaatcaaaa atgatcatcg ggacgcaatt tctcttgcca
    16441 ggttactgcg ggctggtgaa cttacccctg tctggatccc tgacctgact catgaagcta
    16501 tgcgggatct aatcagagcc cgtgcagcag ctaaacgaga cagcagagtt gcccgacagc
    16561 gaattctgag catgctattg cgaacagaca aacgttatgc agggaagcac tggaccggca
    16621 aacatcgaac ctggttagcc aatcaatcat ttagtcagcc atctcaacag attgcttttc
    16681 aacactattg tcagagtctg gagcagattg aagatcgcat acttcaactc gaccaggaaa
    16741 tatcccgcct gttacctgag tggtctctgt gcaatctggt ctgccagtta caggccctta
    16801 aaggtgttgg tcagctaacc gcaataaccc tggttgctga actgggtgat ttttcacgat
    16861 tttccagtcc caaacaactc atggcttttc tgggactggt tccaggggaa tattccagtg
    16921 gaaacagcat tcgtcccagg ggaataacaa aagttggaaa cagcgaactg agacgtctgc
    16981 tttacgaagc cgcctggtct tatcgtacac ctgcaaaagt tggtgcatgg cttatatatt
    17041 accgaccgga ttctgtaaca caatattcca aagatattgc atggaaagct caacaacgat
    17101 tgtgttctcg ttaccgaagt ctgacagcaa aagggaaaaa atcacaagta gccattacgg
    17161 cggtggctcg tgagttgact ggatttatgt gggatattgc acttgctgcc caatcatcat
    17221 tcagtcagca gaagcaaaat taaaccctga agcagtcaga cacggaatga gtgatcctcg
    17281 taaaagaatt caggaggtca gaatgcgcca atacctgcat atagaaagag gccagctcac
    17341 tgcgtaaatc gtcatggggc taaaatcccc gaataagagt ttgtaaaatc caagatacag
    17401 tctgactgtt tcaggttctt taatccgcga tataacgaag ctcccggagc tgcgtaaggt
    17461 ctttttgggc tcaatatttg acaaaggcca taagaattct ttttcccgag acagacggca
    17521 cgaagcgctc tttctgctgt gttattgtcc gcctccgcca gaccgtcatc actgtaataa
    17581 cagagggcgt cccactgatt caggacatag ctgaacgctt cgcccagtct ggattttttc
    17641 gacaacgtgc cattcttctc caccatccat tcatgcagcg acgtcagtaa cgctttgctt
    17701 cgctgctgcc tggctgcaag acgttcagac tccggtaagc cccgtatttc atcctcgatg
    17761 gcgtacagtt cactgatgcg cttcagagct tcttctgccg tcgtactttt gctgctgatg
    17821 tatacatcgt ggatttttcg ccgggcatgg gcccagcacg caacttctgt cagtgcacca
    17881 ccttcacgtt cggcactgaa cagccgctcg taaccgctga acgcatccac ctgcaggata
    17941 ccccggaagg gacgaaggtg ttggtcccgg gactacttta ggtcttggtt cactaataat
    18001 ttcattactg tcaatagccg taatcgccat tgcaggaaat aataaaaagt aagtgtactc
    18061 tgactgcgta tgtatctact ttgtgtaatg caggtgtcta ttgaaaataa ttcttttcgt
    18121 gggcatctgt tgtataaaat aaaaaattat cgtaagtact ccatctgcga tgtcttgtga
    18181 aaattatcct gtctggcaac aatcgcgtcc atctgtatta atggactcga acgatgaact
    18241 ctcaaacaac actagatatc accggctttc gcacttattt gccagactca ttacgtctca
    18301 gattcgacga gcaattatct gtccggacta tcgcacagca agttaagact cagttattcc
    18361 cccattcaca atctctttca acgaattaac gcctcaggta ttgtctgacc atgacccgag
    18421 tccctttcat tcgcccaacc gataccatac tttatgccaa cagaaagaat gaatgaaccg
    18481 cgcctaaaat cagcgaggac acatggcaca aagtgcgaca tgtctcacat ttaaccccag
    18541 tgggggcaac aagtataaaa ccgttttttg attaagtggt cggatatgaa aaggggaagg
    18601 tttatgtgaa aaatacctgt gttttctttg aaaacacaca acagtatcag gttatttatg
    18661 tttatacagt gcgttccaaa ctgacctgaa taattacgca agttgcaaca ataaaaacat
    18721 ttttaccgta gtgtgaggta ctccggtaaa ttgctttcca ggtgcagaca atgactaacg
    18781 aagaactaca ggcaatcgca ttatccatgc ttgagcgcca gcgcgaaaaa gtacaccagc
    18841 aacgagagtc tttgcttaac ccatctccat tcaccaagcc aaaacgattg gagcttgaag
    18901 cattcttgga caaataccca cggcggctta aatcaggcaa aaccagacca ccaggctgat
    18961 agctcacagt ttgttctcct attcaataaa acggataaga accatcagcg cggtagcgta
    19021 aggcactaca gcgcattgct ttacgagtaa taacaatgga tttatcacca gaagacgcaa
    19081 agtttttacg agaactgcaa caccgtatgc tcagagaagc cattatcgaa cagcaaacgg
    19141 gcatgagcgc catagaccca ttgcgataca aacgggcctc atctcctttg gttgacgaca
    19201 attccaatgc ggataaaata cgtccaccac actgattaca tttctcatta gcacagtggt
    19261 gtgatgcagc atacgaggaa aaccggatga atgagaacag aataaaaagg ctggagcaac
    19321 tgttacaagc acgccaacgg gagtttgcca ctaaatgggg acgtgccgcg ctgccacatg
    19381 aacgcctaat ctattttcca cttgaagacc tcattaaaaa taatggcaaa aaaatcaggc
    19441 ctgataagaa acacttctta gaatgcaatg aagacaaata acaacaatct gcatattgcc
    19501 accaaccaca gatgtggcat cgttatcagc gcgcttgatt ttctttatat ccactgacga
    19561 gcttaccggg gcacctttgt ttgccggtca ttctggtatc atgcagatat ttacggcgta
    19621 caagcccact agcatgacat tgtaataagg cctgagccta acgctttaat gtgtgtcata
    19681 cactcagtgg cgtaatcgtt gacaatatgg tgcatgtaat cgtaagcgtc cagcgaaatc
    19741 catctggtca taatctgaag catccgacaa agtggtgtcc accaaataag tgatctgcgc
    19801 ccaatatcct gagccggaac tttgacgaag gccaacgaaa aatgggggcg gatgttacag
    19861 agttctcggt acaggggtaa agtggtacct gtcgccagtt ctccgatctg tttaaccggg
    19921 aaattatctc ctacagcttg tcagaaaggt cggtgatgga gcatcgttaa tacgatgcta
    19981 tacgatgcat tcacagtgcc cggccagagg atgccccgtc gctgcatacg gatcagggtt
    20041 ggcaatatcg aatcgcaggc tataggcaaa gttaaagccc catggagtag cacaaaatat
    20101 gccgcacaaa ggaaacggtc tgaataacgc agtgatggtg aacttcttca gcacactgct
    20161 aaaacgcata gtgatcgagc gctgattctg gcgaacaact gaactaatac atcagaatct
    20221 gcattatgtt aaataaatat gaaaagatgg tttaaatacc ccgttacttt tgacttacac
    20281 tatacggtat cgcatcgttt aatatccgca ccgaccagat ttttatttct attagttgtc
    20341 acaatactga atgcgtacga ccacagtatt ccggctcctg tgtggtcatg ctttaattct
    20401 gcgttccggg cagataagca gttgcttgca ggaatccttc ttgtgttaat gtcagttccc
    20461 ctttttacca gtgctgattt ccacattccg tccaacagag cttatagcct tagcattgtc
    20521 cggctgaagt tctttttgaa taataataga agcactgctg gcagatccag tccgtttttc
    20581 ataacccact gtactgataa ccataatcta atcagtagaa attgagtcga aaataagcac
    20641 tactccatac aggataatta gaggtcagtt tgattattca caattcatca tcagcatttt
    20701 ctgtttctga
//
LOCUS       DAZFQD010000092        20551 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.092, whole genome shotgun sequence.
ACCESSION   DAZFQD010000092 DAZFQD010000000
VERSION     DAZFQD010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20551
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.092"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            70..1047
                     /gene="ybeQ"
                     /locus_tag="ACHKTW_003331"
     CDS             70..1047
                     /gene="ybeQ"
                     /locus_tag="ACHKTW_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YbeQ"
                     /protein_id="HFO6464293.1"
                     /translation="MIFTSSCCDNLSIDEIIERAEKGDCEAQYIVGFYYNRDSAIDSP
                     DDEKAFYWLKLAAEQGHCEAQYSLGQKYTEDKSRHKDNEHAIFWLKKAALQGHTFASN
                     ALGWILDRGEDPNYKEAVVWYQIAAESGMSYAQNNLGWMYRNGNGVAKDYALAFFWYK
                     QAALQGHSDAQNNLADLYEDGKGVAQNETLAAFWYLKSAQQGNRHAQFQIAWDYNAGE
                     GVDQDYKQAMYWYLKAAAQGSVGAYVNIGYMYKHGQGVEKDYQAAFEWFTKAAECNDA
                     TAWYNLAIMYHYGEGRPVDLRQALDLYRKVQSSGTRDVSQEIRETEDLL"
     gene            complement(1117..1599)
                     /gene="ybeL"
                     /locus_tag="ACHKTW_003332"
     CDS             complement(1117..1599)
                     /gene="ybeL"
                     /locus_tag="ACHKTW_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HFO6464294.1"
                     /translation="MNKVAQYYRELVASLSERLRNGERDIDALVEQARERVIKTGELT
                     RTEVDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EVFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGHDQFQRRPFEP
                     "
     gene            1834..4416
                     /gene="leuS"
                     /locus_tag="ACHKTW_003333"
     CDS             1834..4416
                     /gene="leuS"
                     /locus_tag="ACHKTW_003333"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFO6464295.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            4431..5012
                     /gene="lptE"
                     /locus_tag="ACHKTW_003334"
     CDS             4431..5012
                     /gene="lptE"
                     /locus_tag="ACHKTW_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HFO6464296.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTTLGN"
     gene            5012..6043
                     /gene="holA"
                     /locus_tag="ACHKTW_003335"
     CDS             5012..6043
                     /gene="holA"
                     /locus_tag="ACHKTW_003335"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFO6464297.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWIAARAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQTQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            6045..6686
                     /gene="nadD"
                     /locus_tag="ACHKTW_003336"
     CDS             6045..6686
                     /gene="nadD"
                     /locus_tag="ACHKTW_003336"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFO6464298.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            6710..7321
                     /gene="cobC"
                     /locus_tag="ACHKTW_003337"
     CDS             6710..7321
                     /gene="cobC"
                     /locus_tag="ACHKTW_003337"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043755 - alpha-ribazole phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HFO6464299.1"
                     /translation="MRLWLIRHGETQANIDGLYSGHAPTPLTARGIEQAQNLHTLLHG
                     VSFDLVLCSELERAQHTARLVLSDRQLPVQIIPELNEMFFGDWEMRHHRDLMQEDAEN
                     YSAWCNDWQHAIPTNGEGFQAFSQRVERFIARLSEFQHYQNILVVSHQGVLSLLIARL
                     IGMPAEAMWHFRVDQGCWSAIDINQKFATLRVLNSRAIGVENA"
     gene            7581..7898
                     /gene="rsfS"
                     /locus_tag="ACHKTW_003338"
     CDS             7581..7898
                     /gene="rsfS"
                     /locus_tag="ACHKTW_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFO6464300.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            7902..8369
                     /gene="rlmH"
                     /locus_tag="ACHKTW_003339"
     CDS             7902..8369
                     /gene="rlmH"
                     /locus_tag="ACHKTW_003339"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFO6464301.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSTLTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            8400..10301
                     /gene="mrdA"
                     /locus_tag="ACHKTW_003340"
     CDS             8400..10301
                     /gene="mrdA"
                     /locus_tag="ACHKTW_003340"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HFO6464302.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     DPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTAHKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            10304..11416
                     /gene="mrdB"
                     /locus_tag="ACHKTW_003341"
                     /gene_synonym="rodA"
     CDS             10304..11416
                     /gene="mrdB"
                     /locus_tag="ACHKTW_003341"
                     /gene_synonym="rodA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HFO6464303.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            11427..12515
                     /gene="rlpA"
                     /locus_tag="ACHKTW_003342"
     CDS             11427..12515
                     /gene="rlpA"
                     /locus_tag="ACHKTW_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HFO6464304.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTQLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVSGPQGDILP
                     VSNSTLKSEDPIGAPVTSSGFLGAPTTLAPGVLEGSEPTPAPQPVVTAPSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            12654..13865
                     /gene="dacA"
                     /locus_tag="ACHKTW_003343"
     CDS             12654..13865
                     /gene="dacA"
                     /locus_tag="ACHKTW_003343"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HFO6464305.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFGDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            13976..14239
                     /gene="ybeD"
                     /locus_tag="ACHKTW_003344"
     CDS             13976..14239
                     /gene="ybeD"
                     /locus_tag="ACHKTW_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 family protein YbeD"
                     /protein_id="HFO6464306.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            14340..14981
                     /gene="lipB"
                     /locus_tag="ACHKTW_003345"
     CDS             14340..14981
                     /gene="lipB"
                     /locus_tag="ACHKTW_003345"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HFO6464307.1"
                     /translation="MYQDKILVRQLGLQPYEPISQAMHEFTDTRDDSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
     gene            15240..16193
                     /gene="ybeF"
                     /locus_tag="ACHKTW_003346"
     CDS             15240..16193
                     /gene="ybeF"
                     /locus_tag="ACHKTW_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbeF family transcriptional regulator"
                     /protein_id="HFO6464308.1"
                     /translation="MDSNNQIEPCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHK
                     GIVNAAKVLNLTPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLES
                     ILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPTSDAENQL
                     SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICREGNPLLSLEDDRETIDNAAHVLLL
                     PEEQNFSGLRQRVQEMFPDRQINFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWP
                     LEKLPFPSLNEEQIDFSIHYNKFSLRDPILHGVIDVIRNAF"
     gene            16402..17367
                     /gene="lipA"
                     /locus_tag="ACHKTW_003347"
     CDS             16402..17367
                     /gene="lipA"
                     /locus_tag="ACHKTW_003347"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HFO6464309.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(17468..17671)
                     /gene="tatE"
                     /locus_tag="ACHKTW_003348"
     CDS             complement(17468..17671)
                     /gene="tatE"
                     /locus_tag="ACHKTW_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HFO6464310.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(17800..18588)
                     /locus_tag="ACHKTW_003349"
     CDS             complement(17800..18588)
                     /locus_tag="ACHKTW_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HFO6464311.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHVPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRRVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPVLNNRRFAPPQLL"
     gene            18681..19064
                     /gene="crcB"
                     /locus_tag="ACHKTW_003350"
     CDS             18681..19064
                     /gene="crcB"
                     /locus_tag="ACHKTW_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HFO6464312.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTAH"
     gene            complement(19118..19327)
                     /gene="cspE"
                     /locus_tag="ACHKTW_003351"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(19118..19327)
                     /gene="cspE"
                     /locus_tag="ACHKTW_003351"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HFO6464313.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(19502..20062)
                     /gene="pagP"
                     /locus_tag="ACHKTW_003352"
                     /pseudo
     CDS             complement(19502..20062)
                     /gene="pagP"
                     /locus_tag="ACHKTW_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415155.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
BASE COUNT         5231 a         5156 c         5120 g         5044 t
ORIGIN      
        1 taaggcttac gcaaaaatgg aacacataaa aatataatat gttttatctg caaccataag
       61 gaaattataa tgattttcac gtcaagttgc tgcgataatt tatcaataga tgagattatc
      121 gaacgtgctg aaaaaggcga ttgcgaggct caatatattg ttgggtttta ttataatcgc
      181 gatagcgcaa ttgattctcc ggacgacgaa aaagcctttt actggctgaa gctggccgcc
      241 gagcaaggtc attgtgaagc acaatattcc ttaggacaaa agtatactga ggataaaagc
      301 cgtcataaag ataatgagca tgccatcttt tggctgaaaa aagctgccct acaaggccat
      361 actttcgctt ccaacgccct tggctggata ctggatcgtg gagaagaccc caattataaa
      421 gaagcggttg tctggtatca gatagccgcg gagagcggaa tgtcttatgc gcaaaataat
      481 cttgggtgga tgtacagaaa tggcaacgga gtcgcaaaag actatgcgct ggcatttttc
      541 tggtacaaac aagctgcatt acaaggccat agtgacgcgc aaaacaatct ggccgatctt
      601 tatgaagacg gaaaaggcgt tgctcaaaac gagacactcg ccgcattctg gtatttgaaa
      661 agcgcacagc agggtaatcg gcatgctcag tttcaaattg catgggatta taacgctggc
      721 gaaggggtgg accaggacta taagcaagcg atgtactggt atctgaaggc tgccgctcag
      781 gggagcgtcg gcgcttacgt caacatcggt tatatgtata aacacggaca aggcgttgag
      841 aaagattatc aggctgcctt tgaatggttt acgaaagccg ctgaatgcaa tgacgccact
      901 gcctggtata acctggccat tatgtatcac tacggagaag gaagacctgt cgatctccga
      961 caggctctcg acctgtatcg taaagttcag tcatccggaa ccagagatgt cagtcaggaa
     1021 attcgtgaga ctgaagattt actgtagaga taaaaacaag ggccggggaa tttcattcgg
     1081 cccatattct ctttattgct gtgcgaaatc agactattac ggctcaaacg ggcgtctctg
     1141 gaactggtca tgaccacatt tcgggcatag cgtcagcact tccggtgtgt agatcgggag
     1201 atggaagtga catttctcgc agaccagatt tcccagcccg accacttctc cgctgtgata
     1261 aaccccatga tgattgaggt cctggaaaac ttcgcgccat tcaagctgcg ttttatcggt
     1321 gatatctgcc agctcctgcc ataagctttc tttaatcacc cgcataaaga cgctgtcaga
     1381 ttcttctttc aggctctctt catagctcat ggcgaactct tccaggtcac gtcttacagc
     1441 tcgcgtcagc tcatcgacct cggttcgcgt taactcccct gtttttatta cgcgctcgcg
     1501 cgcctgttcc accagtgcgt cgatatcacg ttcgccattg cgcaggcgtt cgctcagtga
     1561 cgcgaccagt tcacggtaat attgagcaac cttgttcatc gtttcatctc ccgggtcgtt
     1621 aactgactat aataatagac gttctttgtt ccttgctttg ctaatacgcc cacaaactct
     1681 gtaaattaat gtatgaataa tccgggcatc atttcaacgt cgagtgcgcg aaaggctgtt
     1741 ttgacgcgga cgtttgggct atgctatgcg gatctgaaaa accacctcaa cgctacattt
     1801 gtagccgtat tgaaaacagg accactggct gccatgcaag agcaataccg cccggaagag
     1861 atagaatcca aagtacagct tcattgggat gagaagcgca catttgaagt aaccgaagac
     1921 gagagcaaag agaagtatta ctgcctgtct atgcttccct atccttctgg tcgactacac
     1981 atgggccacg tacgtaacta caccatcggt gacgtgatcg cccgctacca gcgtatgctg
     2041 ggcaaaaacg tcctgcagcc gatcggctgg gacgcgtttg gtctgcctgc ggaaggcgcg
     2101 gcggtgaaaa acaacaccgc tccggcaccg tggacgtacg acaacatcgc gtatatgaaa
     2161 aaccagctca aaatgctggg ctttggttat gactggagcc gcgagctggc aacctgtacg
     2221 ccggaatact accgttggga acagaaattc ttcaccgagc tgtataaaaa aggcctggta
     2281 tataagaaga cttctgcggt caactggtgc ccgaacgacc agaccgtact ggcgaacgaa
     2341 caagttatcg acggctgctg ctggcgctgc gataccaaag ttgaacgtaa agagatcccg
     2401 cagtggttta tcaaaatcac tgcttacgct gacgagctgc tcaacgatct ggataaactg
     2461 gatcactggc cagacaccgt taaaaccatg cagcgtaact ggatcggtcg ttccgaaggc
     2521 gtggagatca ccttcaacgt taacgactat gacaacacgc tgaccgttta cactacccgc
     2581 ccggacacct ttatgggttg tacctacctg gcggtagctg cgggtcatcc gctggcgcag
     2641 aaagcggcgg aaaataatcc tgaactggcg gcctttattg acgaatgccg taacaccaaa
     2701 gttgccgaag ctgaaatggc gacgatggag aaaaaaggcg tcgatactgg ctttaaagct
     2761 gttcacccat taacgggcga agaaattccc gtttgggcag caaacttcgt attgatggag
     2821 tacggcacgg gcgcagttat ggcggttccg ggtcacgacc agcgcgacta cgagtttgcc
     2881 tctaaatacg gcctgaacat caaaccagtt atcctggctg ctgacggctc tgagccagat
     2941 ctttctcagc aagccctgac tgaaaaaggc gtgctgttca actctggcga attcaacggt
     3001 cttgaccatg aagcggcctt caacgccatc gccgataaac tgactgcgat gggcgttggc
     3061 gagcgtaaag tgaactaccg cctgcgcgac tggggtgttt ctcgtcagcg ttactggggc
     3121 gcgccgattc cgatggtgac gctggaagac ggtaccgtaa tgccgacccc ggacgaccag
     3181 ctgccggtga tcctgccgga agatgtggtg atggacggca ttaccagccc gattaaagca
     3241 gatccggagt gggcaaaaac taccgttaac ggtatgccag cgctgcgtga aaccgacact
     3301 ttcgacacct ttatggagtc ctcctggtac tatgcgcgct acacttgccc agagtacaaa
     3361 gaaggtatgc tggattccga agcggctaac tactggctgc cggtggatat ctacattggt
     3421 ggtatcgaac acgccattat gcacctgctc tacttccgct tcttccacaa actgatgcgt
     3481 gatgcaggca tggtgaactc tgacgaacca gcgaaacagt tgctgtgtca gggtatggtg
     3541 ctggcagatg ccttctacta tgttggcgaa aacggcgaac gtaactgggt ttccccggtt
     3601 gatgctatcg ttgaacgtga cgagaaaggc cgtatcgtga aagcgaaaga tgcggcaggc
     3661 catgaactgg tttataccgg catgagcaaa atgtccaagt cgaagaacaa cggtatcgac
     3721 ccgcaggtga tggttgaacg ttacggcgcg gacaccgttc gtctgtttat gatgtttgct
     3781 tctccggctg atatgactct cgaatggcag gaatccggtg tggaaggggc taaccgcttc
     3841 ctgaaacgtg tctggaaact ggtttacgag cacacagcaa aaggtgatgt tgcggcactg
     3901 aacgttgatg cgctgactga agatcagaaa gcgctgcgtc gcgatgtgca taaaacgatc
     3961 gctaaagtga ccgatgatat cggccgtcgt cagaccttca acaccgcaat tgcggcgatt
     4021 atggagctga tgaacaaact ggcgaaagca ccaaccgatg gcgagcagga tcgcgctctg
     4081 atgcaggaag cgctgctggc cgttgtccgt atgcttaacc cgttcacccc gcacatctgc
     4141 ttcacgctgt ggcaggaact gaaaggcgaa ggcgatatcg acaacgcgcc gtggccggtt
     4201 gctgacgaaa aagcgatggt ggaagactcc acgctggtcg tggtgcaggt taacggtaaa
     4261 gtccgtgcca aaatcaccgt tccggtggac gcaacggaag aacaggttcg cgaacgtgct
     4321 ggccaggaac atctggtagc aaaatatctt gatggcgtta ccgtgcgtaa agtgatttac
     4381 gtaccaggta aactcctcaa tctggtcgtt ggctaagcgc gggaggaagc gtgcgatatc
     4441 tggcaacatt gttgttatct ctggcggtgt taatcaccgc cgggtgtggc tggcatctgc
     4501 gtgataccac gcaggttcct tccactatga aggtcatgat cctggactca ggcgatccga
     4561 acgggccatt aagccgtgcg gtgcgtaacc agttacgtct gaatggtgtc gagttgcttg
     4621 ataaagaaac cacgcgtaag gacgttccat ccctgcgttt gggtaaagtg agcatcgcga
     4681 aagataccgc atcggtattc cgtaacggtc aaacagcaga gtatcagatg atcatgacgg
     4741 tgaatgcgac cgtgttgatc cccggccgtg atatctaccc gattagtgcc aaagtcttcc
     4801 gttcgttctt tgataacccg caaatggcgt tagcgaaaga taacgaacaa gacatgatcg
     4861 taaaagagat gtacgaccgt gctgccgaac agctgattcg taagctgcca agcatccgtg
     4921 ctgcggatat tcgttccgac gaagaacaga cgtcgaccac aacggatact ccggcaacgc
     4981 ctgcacgcgt ctccaccacg ctgggtaact gatgattcgg ttgtacccgg aacaactccg
     5041 cgcgcagctc aatgaagggc tgcgcgcggc gtatctttta cttggtaacg atcctctgtt
     5101 attgcaggaa agccaggacg ctgttcgtca ggtagctgcg gcacaaggat tcgaagaaca
     5161 ccacactttt tccattgatc ccaacactga ctggaatgcg atcttttcgt tatgccaggc
     5221 tatgagtctg tttgccagtc gacaaacgct attgctgttg ttaccagaaa acggaccgaa
     5281 tgcggcgatc aatgaacaac ttctcacact caccggactt ctgcatgacg acctgctgtt
     5341 gatcgtccgc ggtaataaat taagcaaagc gcaagaaaat gccgcctggt ttactgctct
     5401 tgcgaatcgc agcgtgcagg tgacctgtca gacaccggag caggctcagc ttccccgctg
     5461 gattgctgcg cgcgcaaaac agctcaactt agaactggat gacgctgcaa atcaggtgct
     5521 ctgctactgt tatgaaggta acctgctggc gctggctcag gcactggagc gtttatcgct
     5581 gctctggcca gacggcaaat tgacattacc gcgcgttgaa caggcggtga atgatgccgc
     5641 gcatttcacc ccttttcatt gggttgatgc tttgttgatg ggaaaaagta agcgcgcgtt
     5701 gcatattctt cagcaactgc gtctggaagg cagcgaaccg gttattttgt tgcgcacatt
     5761 acaacgtgaa ctgttgttac tggttaacct gaaacgccag tctgcccata cgccactgcg
     5821 tgcgttgttt gataagcatc gggtatggca gaaccgccgg ggcatgatgg gcgaggcgtt
     5881 aaatcgctta agtcagacgc agttacgtca ggccgtgcaa ctcctgacac gaacggaact
     5941 caccctcaaa caagattacg gtcagtcagt gtgggcagag ctggaagggt tatctcttct
     6001 gttgtgccat aaacccctgg cggacgtatt tatcgacggt tgatatgaaa tctttacagg
     6061 ctctgtttgg cggcaccttt gatccggtgc actatggtca tcttaaaccc gtggaaacgc
     6121 tggcgaattt gattggtctg acgcgggtca caatcatccc taataatgtt cctccgcatc
     6181 gtccccagcc ggaagcgaac agcgtgcagc gtaaacacat gcttgaactg gcgattgccg
     6241 acaagccatt atttactctt gatgaacgcg agctaaagcg caatgccccc tcttacactg
     6301 cgcaaacact gaaagagtgg cggcaggaac aagggccgga cgtgccgctg gcgtttatta
     6361 ttggtcagga ttcattgctg acctttccga cctggtacga atacgaaacg atactcgaca
     6421 atgcacattt gatcgtctgt cggcgtccag gttacccact tgaaatggcg caaccgcaat
     6481 accagcaatg gctggaagat catttgacac ataacccgga agatcttcac cttcagcctg
     6541 ccggtaaaat ttatctggct gaaacgccgt ggtttaacat ctcggcgacc atcatccgcg
     6601 aacgtttgca aaacggtgaa tcgtgtgagg atttattgcc ggaaccggta ctgacttaca
     6661 ttaaccaaca aggcttgtat cgctgatacc tgtcgttacg gagggcatga tgcgactgtg
     6721 gttaattcgt catggtgaaa cgcaagcgaa tatcgatggt ctatacagcg gtcatgcgcc
     6781 cacccccctg accgcgcgcg gtattgagca agcgcaaaat ctgcatacgc tgctacatgg
     6841 tgtttccttt gatctggttt tatgcagtga actggaacgg gcacagcata ccgcgcgact
     6901 ggttctcagt gaccgccagc tccccgtgca aatcatccct gaactcaacg aaatgttttt
     6961 tggcgactgg gagatgcgac atcatcgcga cctcatgcaa gaagatgccg aaaactatag
     7021 cgcgtggtgc aatgactggc agcatgcaat ccccacgaac ggtgaaggat ttcaggcatt
     7081 ttcgcaacgt gtggaacgct ttatcgcaag gcttagtgaa tttcagcact atcagaatat
     7141 tttagtcgtc agccatcagg gtgtactgag tctgttaatc gcccgtttaa ttggcatgcc
     7201 tgccgaggct atgtggcatt ttcgcgttga ccaggggtgt tggagcgcca ttgatatcaa
     7261 ccaaaaattc gcgacgctac gcgtcctcaa tagccgtgcc atcggggtcg agaatgcatg
     7321 acttttctgt ttttttacgg gtaagccgca acgaccattg acagacccgg gcaggctgat
     7381 attctccgca gccagacttt tttcgccaga cacgactttg tagaaattgt tttacaaaaa
     7441 tggcgatgca atctgcggcg cggggtggga tgatagccca ctttcgaaag ccgattcggc
     7501 gacaattgtc ccgaaatcgc ctctggttca ggtatactga cagaccattt ttatctattt
     7561 gattcaccca gggggaaaac ttgcagggta aagcactcca ggattttgtt atcgacaaaa
     7621 ttgatgacct caaaggtcag gacatcatcg ccttagacgt tcaaggcaaa tccagcatca
     7681 ccgactgcat gatcatctgt acgggtacgt ccagccgtca tgttatgtcc attgctgacc
     7741 acgttgtgca ggagtctcgc gcagcgggcc tgttaccgct cggcgtagaa ggtgaaaaca
     7801 gcgccgactg gattgtcgtg gatttgggcg atgtgattgt ccatgtcatg caggaagaga
     7861 gccgtcgcct gtatgaactg gaaaaactct ggagttaatg cgtgaagctg caacttgtcg
     7921 ccgtgggaac gaaaatgccg gactgggtac aaaccggttt taccgagtac ctgcgtcgtt
     7981 ttccgaaaga tatgcccttc gagctgattg aaattccggc cggaaaacgc ggcaagaatg
     8041 cggacatcaa gcgcatactc gacaaagagg gtgagcagat gttggcggcc gcaggcaaaa
     8101 accgcattgt caccctcgat attccaggca agccctggga tacgccgcag ttagccgctg
     8161 agctggaacg ctggaagctg gatggtcgcg acgtcagtct actgattggc gggcctgaag
     8221 ggttgtcgcc tgcctgtaaa gcggcggcgg agcagagctg gtcgctgtcg acgcttaccc
     8281 tcccccatcc gctggttcgc gtgctggtcg cagagagtct gtaccgggcg tggagcatca
     8341 ccaccaacca tccttatcac cgtgagtgat aagggagctt tgagtagaaa acgcagcgga
     8401 tgaaactaca gaactctttt cgcgactata cggcagagtc cgcgctgttt gtgcgccggg
     8461 cgctggtcgc ctttttgggg attttgctgc tgaccggcgt gcttatcgcc aacctgtata
     8521 atctgcaaat tgttcgcttt accgattacc agacccgctc taatgaaaac cgcattaagc
     8581 tggtgcctat cgcgcccagc cgcggcatta tctacgaccg taacggtatc cctctggcac
     8641 tcaaccgcac tatctaccag atagaaatga tgccggagaa agtcgataac gtgcagcaaa
     8701 cgctggacgc tttgcgcagc gtggttgatc tgaccgatga cgatattgct gcattccgaa
     8761 aagagcgcgc acgttcacac cgtttcacct ctattcccgt gaaaactaac ctgaccgaag
     8821 tacaagtagc tcgctttgcc gtcaatcagt accgttttcc gggtgtcgaa gttaaaggct
     8881 ataaacgtcg ttactatcct tacggttcgg cgttgaccca cgtcatcggc tatgtgtcga
     8941 aaatcaacga taaagacgtc gaacgcctga ataatgacgg caaactggcc aactatgcgg
     9001 caacgcatga tatcggtaag ctgggcattg agcgttacta tgaagacgtg ctgcacggtc
     9061 agaccggtta tgaagaggtt gaagttaaca accgtgggcg tgttatccgc cagttaaaag
     9121 aagtaccacc gcaagccgga cacgatattt acctgacgct ggatctcaaa ctccagcaat
     9181 atattgaaac gctgctggcg ggtagccgcg cagctgtggt agtcaccgat ccgcgtacag
     9241 gtggggtgct ggcactggtt tccacgccta gttatgaccc aaacctgttt gttgacggta
     9301 tctccagcaa agattattcc gccttgctga acgacccgaa tacaccgctg gtgaaccgcg
     9361 ccacacaggg ggtttatcct cctgcgtcta cagttaaacc ctatgtggcg gtttcggcat
     9421 taagcgccgg ggtgatcacg cgcaatacga cgctgtttga cccaggctgg tggcaactac
     9481 cagggtcgga aaaacgttat cgtgactgga aaaaatgggg ccacgggcgt ctgaatgtca
     9541 caagatcgct ggaagaatct gcggatacct tcttctatca ggtggcctac gatatgggga
     9601 tcgatcgcct ctccgaatgg atgggtaaat tcggttatgg tcattacacc ggtatcgacc
     9661 tggcggaaga acgttccggc aacatgccta cccgcgaatg gaaacagaaa cgctttaaaa
     9721 aaccgtggta tcagggtgac accattccgg ttggtatcgg ccagggttac tggacagcga
     9781 ccccaatcca gatgagtaag gcactgatga tcctgattaa tgacggtatc gtgaaggttc
     9841 cgcatttgct gatgagcacc gccgaagacg gcaaacaggt gccatgggta caaccgcatg
     9901 aaccgcccgt cggcgatatt cattccggtt actgggagct ggcgaaagac ggtatgtacg
     9961 gtgttgctaa ccgccctaac ggtacggcgc ataaatactt tgccagcgca ccgtacaaaa
    10021 ttgcggcgaa atctggtacc gctcaggtct tcggtctgaa agcgaacgaa acctataatg
    10081 cgcacaaaat tgccgagcgt ttacgtgacc acaaactgat gaccgccttt gcgccataca
    10141 acaatccgca agtggctgtc gccatgattc tggagaacgg cggcgcgggt ccggcggttg
    10201 gtacgctgat gcgccagatc ctcgaccaca ttatgctggg tgataacaat accgatctgc
    10261 ctgcggaaaa tcctgcggtt gccgcagcgg aggaccatta atcatgacgg ataatccgaa
    10321 taaaaaaaca ttctgggata aagtccatct cgatcccaca atgctgctga tcttactggc
    10381 attgctggtt tacagcgccc tggttatctg gagcgccagc ggtcaggata ttggcatgat
    10441 ggagcgtaaa ataggccaaa tcgcgatggg tctggtcatc atggtggtga tggcgcaaat
    10501 tcctccacgc gtttatgaag gctgggcccc ctatctctat atcatctgta ttattttgct
    10561 ggtggcggta gatgctttcg gtgccatctc taaaggcgct caacgctggc tggacctcgg
    10621 tattgttcgt tttcagccgt cggaaattgc caaaatagcc gtaccactga tggttgcgcg
    10681 ctttatcaac cgcgacgttt gcccgccatc gttgaagaac actggcatcg cgctggtgct
    10741 gatatttatg cccacgctgc tggtggctgc acagcctgac ctgggaacat caatcctcgt
    10801 tgcgctttcc ggtctgtttg tactgttcct ctctggcctt agctggcgtc tgattggcgt
    10861 cgcagtagtg ctggtggcgg cgttcattcc tattctgtgg ttcttcctga tgcatgatta
    10921 ccagcgccag cgcgtaatga tgctcctgga cccggaatca gacccactcg gcgcgggcta
    10981 tcacattatt cagtctaaaa ttgctattgg ctccggcgga ttacgcggca aaggctggct
    11041 gcatggcact cagtcacaac ttgaatttct acccgaacgc cataccgact ttatcttcgc
    11101 ggtactggcg gaagagctgg gattagtggg cattctgatt ctgctcgctc tctacattct
    11161 gctgatcatg cgcgggctgt ggatagccgc cagagcgcaa accacctttg gtcgcgtcat
    11221 ggctggtggc ttaatgctga tattattcgt ttatgtcttc gtaaatattg gtatggtaag
    11281 cggtattctg ccggttgtag gggttccgct cccactggtc agttatggag gatcggcgct
    11341 aattgtgctg atggctgggt tcgggattgt aatgtcaatc cacacccaca ggaaaatgtt
    11401 gtcgaaaagc gtgtaagagg tgcgcaatgc gtaagcagtg gctcgggatc tgcatcgcgg
    11461 caggaatgct cgcggcatgt acaagcgatg atggtcagca acagacggta agtgtaccgc
    11521 agcctgcggt atgtaacggc cccatagttg aaattagcgg ggcggacccg cgtttcgaac
    11581 cactgaacgc gacggcaaat caggattacc agcgcgacgg taaaagctac aaaatcgtgc
    11641 aggatccgtc tcgatttagc caggcgggac tggcggcaat ctatgatgcc gaaccaggca
    11701 gtaacctgac ggcctctggc gaagctttcg atccgacaca gctgacggcg gcccatccaa
    11761 cgcttccgat ccccagctac gccagaatca ctaacctggc taacgggcga atgatcgtgg
    11821 tgcgcattaa tgatcgcggc ccttacggca acgaccgcgt tatttcgctt tctcgcgcgg
    11881 cagctgaccg tcttaacacg tcaaacaaca ccaaagttcg tatcgatccg attattgtcg
    11941 cccaggatgg ttcgctttct ggtcctggta tggcttgtac cacagtcgcc aaacagactt
    12001 acgccctgcc tgcacctccc gatttaagcg gtggcgcggg aacaagttca gtgtctggcc
    12061 cgcagggtga cattcttccg gtcagtaatt cgacgctaaa aagcgaagat ccgatcggcg
    12121 cgccggtaac cagtagcggt ttcctcggcg ctccaacgac cttagcgcct ggtgtactgg
    12181 aaggcagcga accgacgcct gcgccacagc ccgttgttac agctccgtcg acaacgcctg
    12241 caacctcgcc tgcaatggtg acaccgcaag ccgcctcgca aagcgccagt ggcaacttta
    12301 tggtgcaagt cggggccgta agcgatcagg ctcgtgcgca acagtaccaa cagcaactgg
    12361 gacagaagtt cggcgtcccc ggtcgcgtaa ctcaaaatgg cgcggtctgg cggatccagc
    12421 ttggcccatt cgccagcaaa gccgaagcca gtaccttgca gcaacgtttg caaaccgaag
    12481 cccaattaca gtcatttatt actaccgcgc agtagcgtaa agcaggcatc tgatgtgtca
    12541 atttctgtaa atgacgttaa caaagtcacg cggaaagtca gatgcctgcc ggtagtgcat
    12601 ttgctatagt agggcacttt tttaattcca tcacggatgt cgtagttcag accatgaata
    12661 ccattttttc cgctcgtatc atgaagcgcc tggcgctcac cacggctctt tgcacagcct
    12721 ttatctctgc tgcacatgcc gatgacctga atatcaaaac tatgatcccg ggtgtaccgc
    12781 agatcgatgc ggagtcctac atcctgattg actataactc cggcaaagtg ctcgccgaac
    12841 agaacgcaga tgtccgccgc gatcctgcca gcctgaccaa aatgatgacc agttacgtta
    12901 tcggccaggc aatgaaagcc ggtaaattta aagaaactga tttagtcact atcggcaacg
    12961 acgcatgggc caccggtaac ccggtgttta aaggttcttc gctgatgttc ctcaaaccgg
    13021 gcatgcaggt tccggtttct cagctgatcc gcggtattaa cctgcaatcg ggtaacgatg
    13081 cttgtgtcgc catggccgat tttgccgctg gtagccagga cgcttttgtt ggcttgatga
    13141 acagctacgt taacgcactg ggcctgaaaa atacccactt ccagacggta catggtctgg
    13201 atgctgatgg tcagtacagc tccgcgcgag atatggcgct gatcggccag gcattgatcc
    13261 gtgacgtacc gaatgaatac tcgatctata aagaaaaaga atttacgttt aacggtattc
    13321 gccagctgaa ccgtaacggc ctgttatggg ataacagcct gaatgtcgac ggcatcaaaa
    13381 ccggacacac tgacaaagca ggttacaacc ttgttgcttc tgcgactgaa ggccagatgc
    13441 gcttgatctc tgcggtgatg ggcggacgta cttttaaagg ccgtgaagcc gaaagtaaaa
    13501 aactgctaac ctggggcttc cgtttcttcg aaaccgttaa cccactgaaa gtaggtaaag
    13561 agttcgcctc tgaaccggtt tggtttggtg attctgatcg cgcttcgtta ggtgttgata
    13621 aagacgtgta cctgaccatt ccacgtggtc gcatgaaaga tctgaaagcc agctatgtgc
    13681 tgaacagcag tgaattgcat gcgccgctgc aaaagaatca ggtcgtcgga actatcaact
    13741 tccagcttga tggcaaaacg atcgagcaac gcccgctggt tgtgttgcaa gaaatcccgg
    13801 aaggtaactt cttcggcaaa atcattgatt acattaaatt aatgttccat cactggtttg
    13861 gttaaaaatt gaacacttga aagtgtaatt tccgtcccca tatactaagc atcagtaaaa
    13921 aaactcccgc cttctggcgg gagttgctat ttaattacgt tacgccggag ctgacatgaa
    13981 aaccaaactt aacgaactgc ttgaattccc tactcctttt acttacaaag ttatggggca
    14041 ggcgttacct gagctggttg atcaggtggt tgaagtggta cagcgccatg cgccaggtga
    14101 ctacacccca acggtaaaac caagcagcaa aggcaactac cactcggtat ctatcactat
    14161 caacgccact catatcgagc aggttgaaac actgtatgaa gaactgggca aaatcgatat
    14221 tgtccgcatg gttctgtaac tcgcttctcc gttacccggc tcctggtcgg gtaactcccc
    14281 tctcctcgct gtgatatact tttcccccac ttttactcat tctccacgga gatgccgttt
    14341 tgtatcagga taaaattctt gtccgccagc tcggtcttca gccttacgag ccaatctccc
    14401 aggctatgca tgaattcacc gatacccgcg atgatagtac ccttgatgaa atctggctgg
    14461 tcgagcacta tccggtattc acccaaggtc aggcaggaaa agcggagcac attttaatgc
    14521 cgggtgatat tccggtgatc cagagcgatc gcggtgggca ggtgacttat cacgggccgg
    14581 gacaacaggt gatgtatgtg ttgcttaacc tgaaacgccg taaactcggt gtgcgtgaac
    14641 tggtgacctt gcttgagcaa acagtggtga ataccctggc tgaactgggt atagaagcgc
    14701 atcctcgggc tgacgcgcct ggtgtctatg tcggggaaaa gaaaatttgc tcactgggtt
    14761 tacgaattcg acgcggttgt tcattccacg gtctggcatt aaacgtcaat atggatcttt
    14821 caccattttt acgtattaat ccttgtgggt atgccggaat ggaaatggct aaaatatcac
    14881 aatggaaacc cgaagcgacg actaataata ttgctccacg tttactggaa aatattttag
    14941 cgctactaaa caatccggac ttcgaatata ttaccgctta attccataca tcaatggccc
    15001 aatttacact gggtcattac tcataatacc tgctaatgct ttacatctcc ttcgcgtttg
    15061 tatattttct ttatcacttg cagaaatgcg gtgccacgcc ttgctccgta actcacccca
    15121 ttgattaacc tattgaaata gttgattatg gtgagcaaaa gcgttccgtt ccaattagac
    15181 ataagattat tgtaattttt taaatattat ttttccatga ataaaattgg agtaagtgcg
    15241 tggatagtaa taatcaaatt gaaccctgtt taagcagaaa gtcgtcagaa ggtaaaccac
    15301 aaatatttac aaccttacga aatattgatc ttaaccttct gactattttt gaagctgtat
    15361 atgtacataa agggatcgtt aatgcagcga aagtgcttaa tctgaccccc tcggcaatca
    15421 gtcagtctat tcagaaactg cgcgttatat tccctgaccc attgtttatt cgcaaaggcc
    15481 agggtgtcac tcctaccgca tttgcgatgc atctacatga gtatatcagt cagggccttg
    15541 agtccattct tggcgcgctg gatatcgaag gaagctatga taagcaacga acgataacta
    15601 ttgcgaccac tccctcggtc ggagccctgg tccttcctgt catctatcgg gcgattaaaa
    15661 ctcactatcc gcagctttta ctgcgcaacc cgcccaccag cgacgcggaa aaccaactca
    15721 gtcagtttca aaccgatctc atcatcgata acatgttttg caccaatcgt acggtgcaac
    15781 atcatgttct gttcaccgac aatatggtgt taatttgccg cgagggaaat ccactactct
    15841 ctttagaaga tgacagagag actatcgaca acgctgcgca tgtacttctg ttaccggaag
    15901 agcaaaattt cagcggtctg cggcagagag ttcaagaaat gtttccggac cggcaaatca
    15961 atttcaccag ctacaacatt ttgacaatcg ctgcactggt tgccaacagt gacatgttag
    16021 cgattattcc cagccgtttt tataacctgt ttagccgctg ctggccgctg gaaaaactgc
    16081 cttttccgtc cttaaatgag gagcaaatag atttctctat ccactacaac aaattcagcc
    16141 tgcgtgatcc catcctgcac ggagtaatag atgttatccg taatgcattt tgaaaaagta
    16201 ttgttacagc cagatcacag cgcagaacga taaaatggca caatccaaca acaattttac
    16261 attttcgcga ccgctttggc tgctttcagg tccgtttcaa tgatatactg ccagtcgtta
    16321 attcaaaaat agttgataat tacaacaatc tattgaattg aaacgctttc cttcgtaatt
    16381 cgcaactgga acacgcacgc tatgagtaaa cccattgtga tggaacgcgg tgttaaatac
    16441 cgcgatgccg ataagatggc ccttatcccg gttaaaaacg tggcaacaga gcgcgaagcc
    16501 ctgctgcgca agccggaatg gatgaaaatc aagcttccag cggactctac acgtatccag
    16561 ggcatcaaag ccgcaatgcg caaaaatggc ctgcattctg tctgcgagga agcctcctgc
    16621 cctaacctgg cggaatgctt caaccacggc acagcaacgt ttatgatcct cggcgctatt
    16681 tgtacccgcc gttgtccgtt ctgtgatgtt gcccacggtc gcccggtagc tcctgatgcc
    16741 aatgaaccag tgaaactggc gcagaccatt gccgatatgg cgctgcgtta tgtggttatc
    16801 acctccgttg accgtgatga cctgcgcgat ggcggtgccc agcactttgc ggattgcatt
    16861 actgccattc gggaaaaaag cccgcaaatc aaaattgaaa ctctggtgcc ggatttccgt
    16921 ggtcgtatgg atcgtgctct ggatattctg actgcaacgc caccagatgt gttcaaccat
    16981 aacctggaaa acgtaccgcg tatttaccgt caggtacggc ctggtgcaga ttacaactgg
    17041 tcgctgaagc tgctggaacg ctttaaagaa gcgcatccgg aaatcccgac caagtctggt
    17101 ctgatggtgg gactgggtga aaccaatgaa gaaattattg aggtaatgcg cgacctgcgc
    17161 cgtcatggtg tgacgatgtt aacgctgggg caatatttgc agccaagccg ccatcacctg
    17221 ccggttcaac gttacgttag cccggatgag ttcgacgaaa tgaaagccga agcgctggcg
    17281 atgggcttta cccatgctgc atgcggtccg tttgtccgct cttcttacca cgccgatttg
    17341 caggcgaaag ggatggaagt taagtaatcc tgtaataatt acttacatct gtctgataaa
    17401 aaacccgctt tttgaagcgg gttttttgta tcgaacaaga tattgaggga gcgtcctgct
    17461 cgccacgtca ctctttatga gagagctttt cagcctgaag atcaacgtct gcgccttttt
    17521 tcgccgcagc atcgtcatca ttcatcgcct tcttgaaccc tttaatggcc gctccaaggt
    17581 ctccgcccag cgtacgtaac ttcttagtcc caaacagcag aacgaccagc gccgcaacta
    17641 ccagcagttt ggtaatacta atctcaccca tagatacctt cttgacatat aaccggcaac
    17701 agaacgcgcc ttaaaaacca gagaatatta acggtcattc gacgcgcgca cacaatagca
    17761 atctgtaaca aggtgaatca agcgccgagt aaaaaaacat cataataatt gcggcggcgc
    17821 aaagcgacgg ttgtttaaga cgggcagttg cgcgcgcacc tgacgcacac gttcgggcgt
    17881 cacttccgcc ataatgagtg caggcatttc tgacgctgcc gcaatggtga cgccaaaggg
    17941 atctataatc cggctttgac cgatattttt gttcccgcac tcccccgccg ccaccatata
    18001 acaggtggta tccagcgcac gagcggcaag caacgttgac caatgatgct ctttgagcgg
    18061 cccgcgaacc caggcggcag gaagtaccag gatttcagct ccctgtaatg cctgtgccag
    18121 cgccagctct ggaaagcgta agtcataaca ggtcatcaga ccgaccttca tcccctccac
    18181 ctcgagtaac ggagcgattt cattaccagc atcaacacgg cgtgattcct gaatggcaaa
    18241 tgcatcatag agatgcaatt tggcataacg ggcgacgatg tttcctgcct gtagcgccac
    18301 cagcatattc catgcgcgcc ccggcgttga aggaacatga atcgtcagaa ttgtcgtcat
    18361 catgttacgt ttactttctc gccgtaaacg tccgaggaat tcgccttcca gcagctgtgc
    18421 tgatttaacc gatagatctg catcatgatc atcgcgcgcc agcaatgctt ccggcaggac
    18481 gaacagcgat acgtcgtttt ccgccgcctg cgccatcaac gaggcacaaa tctcagcgtt
    18541 cttttcccac acagatgtaa cagcaaactg tcctgcggca accagcattg ccacctcctg
    18601 tcagagtcgt aaaattcgcc aacgacgatt attcggcgtt acacttatca ctcatacaaa
    18661 tcaaatagca ggattttgca gtgttacaac ttcttttagc agtttttatt ggcggtggta
    18721 cgggaagcgt ggcgagatgg ctgttaagta tgcgatttaa cccgctgcat caggcgattc
    18781 cgttggggac gctggcagca aacctgattg gggcattcat cataggaatg ggattcgcct
    18841 ggttcagcag gatgacgaac attgatccag tgtggaaagt attaatcacc accggatttt
    18901 gtggcggtct aacaaccttc tcaacatttt cggcagaagt ggtgtttttg ttacaagagg
    18961 gccgctttgg ctgggcgtta ctgaacgttt tcgtcaacct tctggggtct tttgccatga
    19021 cagcactggc tttctggctg ttttcggcct caaccgcaca ctaaagacca aaaaaaaccc
    19081 gctgattaag cgggttttga attcttgctg acgtatctta cagagcgatt acgtttgcag
    19141 cagaagggcc tttggcaccg ttagtgattt cgaactctac gcgctgacct tcagcaagag
    19201 ttttaaaacc attagtctgg attgcagaga agtgtacgaa cacgtctttg ctgccgtctt
    19261 ccggagtaat gaaaccgaat cctttggact cattaaacca cttaacgtta cctttaatct
    19321 tagacatcaa aattaccttt acatgaaaaa tagacacaaa tgctgtgtcg gttaccagta
    19381 caccaattgt ggtacgcttt gtccagtgca actttactaa aaagtgataa aagtcgcgtt
    19441 atttttgatg ttacttattt tagctattga tttttaagaa gttactaaaa cttcatttgt
    19501 ctcaaaactg aaagcgcatc caggcaaagt acacattgcc attgttgtag gtacccggaa
    19561 tgtaggtcat ctgaaaagtc actgggccat aacccacgga ggccaatggc agtagaaccg
    19621 ggagagggat gtaattccag ttatcgcgtg ccgttacgcc agcggtgaat cccagaccta
    19681 aatgaaaatt ttcatccgcc agcggtcgcc aggtactttc ccatccgtat ccggcaatcg
    19741 gttcccattt gttccacgag tccttaaatg ccatggcata caggccatgc cagtttcctt
    19801 tttcatccca acgcgacagg ccaaaaccgc caccccacgg tcgctcgtta tagcgatcgg
    19861 ttttttcttt gtcgtaagcg aaacgtgcat gccaggtgat ggcaggaata tataaatcat
    19921 aatgttcagg ctgttgccag gtttgtgcaa tattttatct aaatgttgtc atccactcat
    19981 ctgcgttagc aaaaacttta ccaacgctga ttaactgaat aaaaacaaag gaaaagatag
    20041 cgacatattt actcacgttc atttgtgacc ataaaacatt tatcaaaaat ctactactag
    20101 catagcaaag ctacaattaa cataacctta atagacccaa cataaagaat aatctgaata
    20161 gctgctctat ttatttccgc ttgttcaagt taaatatttc cgcgcatcga ttaaagatga
    20221 tgcgtcgttt tctgcgatgg gaatagtcaa aaaagaaaaa ccaagtcttt tttgatgaca
    20281 aaatgcaatc aaggaaaaat tattttattt tttaacgaga tacaacaatc atcttaacga
    20341 agtatataat atatatcgtc aacctatgct catattagat tattttttga gcgcacagcc
    20401 gcagctaaca ccattgcaat taacaaattt gcatcaatcc accatcaatt tgcacacatt
    20461 attatgtgat aattgccaac cgctaaatat gcgttttgtt atctatgtat aaaaacagca
    20521 acttcaatgt cttaatggca gtttttcttg a
//
LOCUS       DAZFQD010000093        20539 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.093, whole genome shotgun sequence.
ACCESSION   DAZFQD010000093 DAZFQD010000000
VERSION     DAZFQD010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20539
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.093"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..356
                     /gene="ydjO"
                     /locus_tag="ACHKTW_003353"
                     /pseudo
     CDS             1..356
                     /gene="ydjO"
                     /locus_tag="ACHKTW_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001347595.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="protein YdjO"
     gene            complement(360..1751)
                     /gene="tcyP"
                     /locus_tag="ACHKTW_003354"
     CDS             complement(360..1751)
                     /gene="tcyP"
                     /locus_tag="ACHKTW_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
                     /protein_id="HFO6464314.1"
                     /translation="MNFPLIANIVVFVVLLFALAQTRHKQWSLAKKVLVGLVMGVVFG
                     LALHTIYGSDSQVLKDSVQWFNIVGNGYVQLLQMIVMPLVFASILSAVARLHNASQLG
                     KISFLTIGTLLFTTLIAALVGVLVTNLFGLTAEGLVQGGAETARLNAIESNYVGKVSD
                     LSVPQLVLSFIPKNPFADLTGANPTSIISVVIFAAFLGVAALKLLKDDAPKGERVLAA
                     IDTLQSWVMKLVRLVMQLTPYGVLALMTKVVAGSNLQDIIKLGSFVVASYLGLLIMFA
                     VHGILLGINGVSPLKYFRKVWPVLTFAFTSRSSAASIPLNVEAQTRRLGVPESIASFA
                     ASFGATIGQNGCAGLYPAMLAVMVAPTVGINPLDPMWIATLVGIVTVSSAGVAGVGGG
                     ATFAALIVLPAMGLPVTLVALLISVEPLIDMGRTALNVSGSMTAGTLTSQWLKQTDKA
                     ILDSEDDAELAHR"
     gene            complement(1884..2474)
                     /gene="ydjM"
                     /locus_tag="ACHKTW_003355"
     CDS             complement(1884..2474)
                     /gene="ydjM"
                     /locus_tag="ACHKTW_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFO6464315.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPEGWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            complement(2637..3305)
                     /gene="hxpB"
                     /locus_tag="ACHKTW_003356"
     CDS             complement(2637..3305)
                     /gene="hxpB"
                     /locus_tag="ACHKTW_003356"
                     /EC_number="3.1.3.22"
                     /EC_number="3.1.3.50"
                     /EC_number="3.1.3.68"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HFO6464316.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            3452..3988
                     /gene="yniB"
                     /locus_tag="ACHKTW_003357"
     CDS             3452..3988
                     /gene="yniB"
                     /locus_tag="ACHKTW_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HFO6464317.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFTHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            complement(4029..4889)
                     /gene="yniA"
                     /locus_tag="ACHKTW_003358"
     CDS             complement(4029..4889)
                     /gene="yniA"
                     /locus_tag="ACHKTW_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HFO6464318.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDTIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACYWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(4995..5285)
                     /gene="ghoS"
                     /locus_tag="ACHKTW_003359"
     CDS             complement(4995..5285)
                     /gene="ghoS"
                     /locus_tag="ACHKTW_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HFO6464319.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            complement(5386..6315)
                     /gene="pfkB"
                     /locus_tag="ACHKTW_003360"
     CDS             complement(5386..6315)
                     /gene="pfkB"
                     /locus_tag="ACHKTW_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HFO6464320.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQNQG
                     IRCIIDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
     gene            6602..7360
                     /gene="ydiY"
                     /locus_tag="ACHKTW_003361"
     CDS             6602..7360
                     /gene="ydiY"
                     /locus_tag="ACHKTW_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HFO6464321.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVSY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
     gene            complement(7413..7520)
                     /gene="yniD"
                     /locus_tag="ACHKTW_003362"
     CDS             complement(7413..7520)
                     /gene="yniD"
                     /locus_tag="ACHKTW_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YniD"
                     /protein_id="HFO6464322.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(7696..9595)
                     /gene="espL1"
                     /locus_tag="ACHKTW_003363"
                     /pseudo
     CDS             complement(7696..9595)
                     /gene="espL1"
                     /locus_tag="ACHKTW_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
     gene            10119..12047
                     /gene="thrS"
                     /locus_tag="ACHKTW_003364"
     CDS             10119..12047
                     /gene="thrS"
                     /locus_tag="ACHKTW_003364"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFO6464323.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEDGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            12051..12593
                     /gene="infC"
                     /locus_tag="ACHKTW_003365"
     CDS             12051..12593
                     /gene="infC"
                     /locus_tag="ACHKTW_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFO6464324.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPGT
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            12690..12887
                     /gene="rpmI"
                     /locus_tag="ACHKTW_003366"
     CDS             12690..12887
                     /gene="rpmI"
                     /locus_tag="ACHKTW_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFO6464325.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            12940..13296
                     /gene="rplT"
                     /locus_tag="ACHKTW_003367"
     CDS             12940..13296
                     /gene="rplT"
                     /locus_tag="ACHKTW_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFO6464326.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTALVEKAKAALA"
     misc_feature    13413..13537
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            13419..13463
                     /gene="pheM"
                     /locus_tag="ACHKTW_003368"
     CDS             13419..13463
                     /gene="pheM"
                     /locus_tag="ACHKTW_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HFO6464327.1"
                     /translation="MNAAIFRFFFYFST"
     gene            13747..14730
                     /gene="pheS"
                     /locus_tag="ACHKTW_003369"
     CDS             13747..14730
                     /gene="pheS"
                     /locus_tag="ACHKTW_003369"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFO6464328.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DTTRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            14745..17132
                     /gene="pheT"
                     /locus_tag="ACHKTW_003370"
     CDS             14745..17132
                     /gene="pheT"
                     /locus_tag="ACHKTW_003370"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFO6464329.1"
                     /translation="MKFSELWLREWVNPAIDSDALANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVTVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPALQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLPSPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            17137..17436
                     /gene="ihfA"
                     /locus_tag="ACHKTW_003371"
     CDS             17137..17436
                     /gene="ihfA"
                     /locus_tag="ACHKTW_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HFO6464330.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            17537..18517
                     /gene="btuC"
                     /locus_tag="ACHKTW_003372"
     CDS             17537..18517
                     /gene="btuC"
                     /locus_tag="ACHKTW_003372"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HFO6464331.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     SPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPLWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASALLLADVVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR"
     gene            18580..19131
                     /gene="btuE"
                     /locus_tag="ACHKTW_003373"
     CDS             18580..19131
                     /gene="btuE"
                     /locus_tag="ACHKTW_003373"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HFO6464332.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTAVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGLVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            19131..19880
                     /gene="btuD"
                     /locus_tag="ACHKTW_003374"
     CDS             19131..19880
                     /gene="btuD"
                     /locus_tag="ACHKTW_003374"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HFO6464333.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            19958..20422
                     /gene="nlpC"
                     /locus_tag="ACHKTW_003375"
     CDS             19958..20422
                     /gene="nlpC"
                     /locus_tag="ACHKTW_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFO6464334.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLQLPRETKEQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
BASE COUNT         5307 a         4775 c         5224 g         5233 t
ORIGIN      
        1 tgccgggagc atttttgctg cgattcaaaa aaaatgagag tctcctaaag tttccttcga
       61 taatatcaat gcttaatatc aatcttcttt ttaatccact aaatcttccg ggaatgggaa
      121 gtggtgtgct tgaagatatt atgagtattc cagatagttc acttagaaaa cgtctcgggt
      181 atgaggtttt aagcttctca ctacaggcac attcattgag ccaggaatgt attgataagt
      241 tggacatttt tttcgctgac gatctgttta aatatgagtc agtatgcatc gccgcgatgg
      301 aacatttaaa aagtaaagca acggccccta tacaaaacgg accgttgcca gcataagaat
      361 taacggtgtg ccagttcggc gtcgtcttcg ctatccagaa tggctttatc ggtttgcttc
      421 agccactggc tggtcagcgt gccagctgtc atcgagccac taacgtttag cgccgtacgg
      481 cccatgtcga taagcggttc aacggagatt aacagcgcca ccagggttac tggcaggccc
      541 atcgcaggca gcacaatcag tgcggcgaaa gttgcaccac caccgacacc ggcaacgcct
      601 gcggaactaa cggtaacaat accgaccagc gtcgcaatcc acatcgggtc cagcgggtta
      661 atgccaaccg taggcgcaac catcaccgcc agcattgccg gatacaaacc ggcgcagccg
      721 ttctgaccaa tggttgcacc gaaagaggcg gcgaaactgg cgatggattc aggaacgccc
      781 agacgacgcg tttgtgcttc cacattcagt gggatagacg cagcactgga acggctggta
      841 aaggcaaacg tcagcacagg ccatacctta cggaagtact tcagcggact cacgccatta
      901 atgcccagca gaatgccatg cactgcaaac ataatcagca gaccgaggta ggacgcgaca
      961 acgaaacttc ccagtttgat gatgtcttgc aagttagaac ctgcaaccac tttggtcatt
     1021 agagccagaa cgccgtaagg ggtcaattgc atgacaaggc gaaccagttt catcacccag
     1081 ctttgtaggg tatcgatagc ggctaagacg cgttcacctt tcggcgcatc atccttcagc
     1141 agtttcaatg cagctacgcc gaggaatgcg gcaaaaatta ccacgctgat aattgacgtc
     1201 ggattggctc cggtaagatc ggcaaacggg tttttcggga taaaggacaa gaccagctgc
     1261 ggaacgctca gatcagagac tttaccaaca tagttacttt caatggcgtt cagacgtgca
     1321 gtttctgcac caccctgaac cagaccttca gccgtcaaac caaacaggtt ggtgaccagc
     1381 acaccgacca gcgccgcaat cagcgtggta aacaaaagcg taccgatggt cagaaaactg
     1441 attttgccta actgagatgc gttatgcaga cgggcaaccg cgctcagaat agaggcgaag
     1501 actaacggca taacgatcat ttgcagcagt tgaacatagc cgttaccaac gatgttaaac
     1561 cactgtacag aatctttaag tacctggctg tcagaaccat aaatggtatg cagggcaagg
     1621 ccaaaaacca cacccatcac cagacccacc agcacttttt tcgccagact ccactgttta
     1681 tggcgggtct gagccagcgc aaacagcagt acaacgaaca ccacgatgtt cgcaattaat
     1741 ggaaagttca tccccgttct cctgatctta ttatttctat cactgcgtag cagtcatagt
     1801 gttggcgcaa gattaacaga actgtgagct ggctcttata ttcaaatgga attgcttatt
     1861 ccagaaaggt gatttttgtc tttttagtat tcaacctgat gcttaataag ccgatgaaac
     1921 tggatctgca aggtattgat catttgcgat gaccaacgaa cagcgctgtt ctcgggtaat
     1981 gaatggggca tccataccga ccagacaaat aatgccatgc agataaaacg ttccagttgg
     2041 ttgccctttt ggggaaccag gataggcaag cggaaacgcc agcggcatgg ccagagcagg
     2101 ggaacaccgg cgggtgtcag catatcggca agtatgtggc tcaaataacc cagcaccatt
     2161 ccttgtagcg catcagccgg aatgaaccag ccttccggaa ccttaaggta aaaggttgcc
     2221 agcagcgcaa ataccgccag cagactgtgg gtaaaaccac gatgcccaaa agcgcgggcg
     2281 atcggttttg atatccattt taatcgctgc ccaagaaacg actttgggtg atcgatgtcc
     2341 ggtaacaaac acgtcaggat tgcggaaggg acaatatgcc accagtcacc ctgtgccagc
     2401 acgggcgtca gctcggcatt tttggcaaat accgcacaag caatagaaaa gagaaggtga
     2461 ccttccgccg tcatgataaa actcactaaa ctgtcgatac gtacagtata ggatttttat
     2521 acagtgcacg gaagtggtga cggtgtaact ctttctctta aattgacgat taatccgtca
     2581 gttgaatgca aattcagata aagaaccagg ggcagcgaga tcactgcccc tggtcattaa
     2641 ccgagaaggt cttttgcggt gagttctgtc agcgatgaga gtttgacgtt tgctaataca
     2701 aaacgtggat cattttgcgc ttctggcgca ggaacgacaa tggaacgcat gcgggctgct
     2761 ttagaggcga tcatgccatt tactgaatct tccagcgcta cgcaggtcag tgggtcaacg
     2821 cccagttttg ctgcgcagtc gagatatact tgcggatgcg gcttgctgta aggcagtttt
     2881 tcagccgagg cgagggcatc gaaactgtcg cgtaagtcaa acatggtcaa cactttttcc
     2941 agcatatgta gtggtgacgc ggaggccagt cccaccaata aaccttgttc tttgcataac
     3001 gcaacggctt cgcgcacgcc tggtaataat ggacgtgtct cttcaaccag tgaaatggca
     3061 cgggcaataa cccgttctac tacttcctga cggcttggcc cattccacgg ttgccgggcg
     3121 taccaaagat cgaccaccat atcgatgcgt aaacctaagg tgtccggcag ctcgttacga
     3181 cgggagatat ccacccccag gcttgccatc acatccagtt cggctcgatc ccataaaggt
     3241 tctgagtcga taagtaatcc atccatatca aaaattgcag caagaatctg acgcggggtt
     3301 gacattacaa cctctccttt agagcattat gtgtaaggta ttaagccgtg cttatgatta
     3361 agcgcctatc atatcgcctt taaagaatgg gcgaaaacag atatctacag gtaaactaag
     3421 tgaaacgacg cgtcttaatc aaggagaact catgacgtat caacaagctg gacgcattgc
     3481 tgttttgaaa aggattttgg gttgggtgat ttttatacct gcattgatct ctacattgat
     3541 ttctttgctg aaatttatga atacccggca ggaaaaccag gaaggcatta atgcggtcat
     3601 gctcgacttt actcatgtca tgatcgatat gatgcaggcg aatacgcctt ttttgaatct
     3661 gttctggtat aactccccga cgcccaattt taatggtggc gtgaacgtta tgttctgggt
     3721 gattttcatc ctgatttttg tcggactggc gttgcaggat tccggtgccc ggatgagtcg
     3781 ccaggcgcgc ttcttgcggg aaggtgttga agatcaactc attctggaaa aagccaaagg
     3841 ggaagagggg ctaacgcgtg aacaaattga gtcccgtatt gttgttccgc accatactat
     3901 tttcctgcag tttttctcac tatatatcct gccggttatt tgtattgctg caggttatgt
     3961 gttcttttct ctgcttgggt ttatttaatc aaaaggccgc acggagcggc cattatcctc
     4021 aacccatatc atgctgctaa taatctatcc aatgactgct gagcaataac caaatgctga
     4081 ccgccaaata atcttgcacg atttaacagc gtgtagagtt ggtaaaccgg ttgacgttca
     4141 aggaaatcgg caggtagcgg ggatactgac tgatagccgt catagatttg tggcggctgt
     4201 tcagtatgca gcggtaacat cgccaggtcg cactctcggt caccccagta gcaggcaggg
     4261 tcgaaaatgt acgggccatc cggacccagc gcacagttgc cggaccataa atcgccgtgc
     4321 aacagagaag gctgcggttg atgcgaggcc agacgctgct gaatatgctc gacgatggtg
     4381 tcgatattgc cgaaagcgat ccctttctct gctgccagtt ccaactgcca gccaatccgt
     4441 tgttcagcaa aaaacgttga ccagcgacgt tgccaggtgt tgggctgtgg agttgtggag
     4501 agcgagttat caaaatcgag gccaaattgt ggttggtcac tccattgatg taaacgcgca
     4561 atttgctgac caagaataaa tgcgctatgc gcatccagcg gacgaggtgg gagataatcc
     4621 atcaccagaa aactgtagtc acggtcagcg cctactgccc aaaccttagg cacggtgacg
     4681 gttttactac gcgacagtaa ctccagttgg tcggcttcgg cggtaaaacc gggaagcagt
     4741 tccctttcat cacatttgac gaaaaagtca tgtcctgcat agcgcaaatg ccatgcggca
     4801 tggacttctc cgccaggcag ttcattacgc agttcgattt cgccttcacc taactgctcg
     4861 ctcaaaagac gactgattgc ctgccacatg atgtctctcc catgttgtct gccagatcat
     4921 aaggttagcg caagaatgcg gtgtaaaaac acgagctaac gcacaccaca gactgattat
     4981 tgcccaaaaa ccatttattt tctgttgcta ttccattcct cccaggtcgt gatggtgatt
     5041 tcaggatctt tgccggttgc actttgggta agccccgaaa ccagttctct aatttcttct
     5101 tcactttggg tactgataag tccaaaagtg ttagtcccca gctcatggat atttccctca
     5161 tcatccgtca tggtgagcaa aaaaccgtcg cgagtcaggt aattattcaa ctcgttaatt
     5221 tcagtcaatg tatcctcatg caacatgacg gttatgacat aacggacaag atcgccactg
     5281 gccatagttc acccctttgt tatcacatga cttttgagca tagtcggaga aacgcgttgc
     5341 cgacaggttg gtggtgattc ccccaatgct gggggaatgt ttttgttagc gggaaaggta
     5401 agcgtaaatt ttttgcgtat cgtcatggga gcacagacgt gttccctgat tgagtgtggc
     5461 tgcactcccc gcagctacgc caaaacggac catctcttca agagaggcat tttctgccag
     5521 tttcagtgtc atcgcgccga ccatgctgtc accagcgcca acggtactct ggcttttcac
     5581 cggtggtggc accacctgaa tgcagttttc actatcaaca cccagcgctc cttgtggacc
     5641 aagggaaacg acaacccgtt tggccttgcc gctattaacg atttcctgcg cggctttgcg
     5701 gacatcgtct ggctgggtga gttcgcgatt caccagcgca ctgagttctt tttggttagg
     5761 cttaaccaac tcgatgttac caatcgccag tgctgcactt aacgcttcgc cagaactgtc
     5821 gatgatgcag cggatccctt ggttttgcgc agcggaaatc agttgggtta atttttccag
     5881 cttcacacct ggcggcaggc ttccgcttat gaccaggatg gccccggatt caatttccag
     5941 aacttgctct tcaagctggc gaaactcatc ttcatttaat gccgcgcctg gcataacaaa
     6001 acgatactgc tcaccgcttg cttccacatg tacgtgtaaa ttctgccggg tccagtcttt
     6061 ggcttctaca gtagcgacgg ggacattttc atccgccaac agagaaacca ggtgttcgcc
     6121 ggtcgcgcca cccgccggga agatcgctgt ggcactgcct ccaagatggg caatggcgcg
     6181 ggcgacgttg atgccgccgc ccccgggttc gaacaccggt gcggtacagc gcagttttcc
     6241 ttcgggataa atttgcgggg taattgttgc gctatcgaga gagggcgcaa gtgtcaacgt
     6301 atagatacgt accatcattt cctcctatag gctgatttca gtttggcacc gaatcagccg
     6361 aaagtgaaga aattaacaat atgatttatt agagctttat ttaaaatttt gcagataaat
     6421 atatataaat aaaaatctgt tttcgccaca tgcttattca aaaaatgaaa taagaataca
     6481 ttgctaagtt attcgcgcct ttttataatt cgttaccttt ccagggacgc taatctttga
     6541 tccttaagaa agtttccggg accgcgatgg tctctttttt tgttgtacgg actcttgata
     6601 aatgaagctt ttgaagacag ttcctgccat cgttatgttg gcggggggca tgtttgcgtc
     6661 actgaatgca gctgccgatg attccgtttt tactgtcatg gacgatcctg ccagcgctaa
     6721 aaaacctttc gaaggtaatc tgaacgcagg atatcttgca caatcaggca atacgaaaag
     6781 ctcctcactt acggctgaca ccaccatgac ctggtatgga caaaccactg cctggtcgct
     6841 gtgggggaac gccagtaata cttcttctaa tgatgagcgt tcttcagaaa aatatgcggc
     6901 gggcgggcgt agccgtttta atttaactga ttatgactat ttatttggtc aggcaagctg
     6961 gctgacagac cgttataacg gctatcgaga gcgcgatgtg ttgaccgcgg gttatggtcg
     7021 tcagtttttg aatggcccgg tacacagttt ccgctttgaa ttcggtccag gtgtgagtta
     7081 cgacaaatat accgacaatg ccagtgaaac tcagccgctg ggctatgcct ccggtgctta
     7141 tgcatggcag ttgactgaca acgctaaatt tacgcagggt gtttccgtct ttggcgcgga
     7201 agacacaacg ctgaactccg aaagcgcatt aaatgttgct attaatgaac attttggact
     7261 gaaagtggct tacaacgtca catggaactc tgaacctcca gagtctgcac cggaacatac
     7321 cgatcgtcgg acaaccttat cgctgggcta cagcatgtaa aattatctgc cggaccgtgt
     7381 tatgcggtct ggctgtcttc gtttgtcact gctcagcccc aaatcattgc tgcccaacgt
     7441 agcaacaaca tagcgccaca gattgccagt agcaccacca ccatcttcca gtgtttttta
     7501 tcaaagcgtt tagtcggcat accagtgtcc tgcatatctc ttgtgcgtga taacggagtg
     7561 taacaaaagg gacaggcatc aagggatttt ttgttattac agccgtcggg tggcgttgta
     7621 cacctgatca ctgtttctgc gttttatgaa aaaaataaaa ttatgtatcg atatacaaat
     7681 atcataaata gctggttata aatacgtttt tagttcatca gcactaaaac tcaggctttg
     7741 gctgattgca ttaatagcag cataatatgt ttctacagtt ttataatgat tatgatgaat
     7801 ggctatatga acagctgaca tgttttcatg atttttagcg gccaacagtg tgaataattg
     7861 acgttgacta aaagaatatt tttttgcaaa agtactcagt gttgatatat aagataacac
     7921 gacgtcttta ttacccttgc tcatggcgat gtataaagca ggggttccat gtgctgtcgc
     7981 gccttttaat aagtccatga tgttgatttt gctgagttta tatttaacgg cgatcccata
     8041 aactttagag aggaaccgtg tgacacacaa agggtgatta ttttccattg cagcaaataa
     8101 ccctggttca ccatcacgtg ttttcatggc taaaatatcc atcaccattt tttttgttta
     8161 aaagttcttg tgctattgga gtggttaatg cattcagaaa gatatcgatt atttcatcga
     8221 atccatacaa catggagata tagagtccag gtgtttcgtt agcactttta gtttgcagta
     8281 atttaaccag attatcttca tgaatgatat gattgtatac cagtgactgg atttcttgca
     8341 taaatatttt tacgttgtca gtttgtccgt tcatcatagc cagaaataaa gcaggtgtac
     8401 caacgctatt ctgcgcagtt attatttcaa tgatttcttt ttcagggagt atgggcaact
     8461 gtttgaatag tgtaataagc tattgagtct ggcctgtggc cattattaac aacattgccg
     8521 atggatgtat aggggcaaat ggctttcgtg tcgtgatacc cgctaaaata gcaactggaa
     8581 ctccctcagg taatagagga agaatagcaa ttgcacagtt gcgaacatac ctatcagccc
     8641 aggtctgagc atcgggagct gaactgatga acttatcaag cgagagaaag cgccgtgctg
     8701 tcctgtgggt gcctctgacg gcaacgttgg tatcgttcgg gtcgtaaaca gacacaacat
     8761 aatttaattg tccatacact gactgcttta ttcgtagcct tactgacaaa gtatgattgt
     8821 ctatatataa aatcgcggac atatgactga cattgtttgc catcattcga caaaagaaaa
     8881 tatctaaagc gaagccaaaa tccttactcg cgataacgcg actccctgca ggaggatggc
     8941 gaagtaatgc tctgaactcc gcatcccgga ctaaggggat atgctgctgt atggattcaa
     9001 cggaggagta atgtttaatc aggttgactt tgccttttcc atttctcaat gaatcaaggg
     9061 cgtattgcaa tgacagatgg cgacaaaaaa tagcgtcaga aggagattgc aaaaaacatg
     9121 cactaccatt gagatttact gttgggtgta tagttttttc tctatggatt tttttagaca
     9181 gtttcataca atggttacga tcttcagcag ttaactgttc ctgttcaata taatccagta
     9241 tgcttcgcaa atggttagct ttaataggcg tggtttccag taataaggaa atgaaatctt
     9301 ttttatagtg taatgtaatg agtatatttt ctgcatggat gggtgaagtg gcaaagttaa
     9361 tggtaaaaag ggcagtggaa taacaatttt gtgatatttt tcgacagttt gcggtaaatt
     9421 cctgaataac acagtatgac agcatcgaac ttaagtcaga ggtatcaagt agggtgatga
     9481 gctggcgcgc aaatttatta ataggctctg atgaaggtgt tggtatgtcg ctatcgatgt
     9541 gcaatctatg aggagactta taatgagttc tgataatgat atcgttttga ctcataatct
     9601 attcgcctta tatatatctg gaggttttga ggatgatgat taattattat cgccagaacg
     9661 aaatgtgcac gaaaaattgt tataagaatt gtgtcacttt tatcattgtg atatgaccac
     9721 ttcgtcatta atctcatgag cgtttaaaat actaccgcca aatcacgcaa cgaactttgc
     9781 tggattcagt ttatgctgct gtaaatccgc tcgcagtaaa cctttcagac gcaccgtgat
     9841 gttatcagtg gttctttaag cgttttgctg gtgtactcac tacaaacgaa ttgcgaatcg
     9901 aatcaatgtg aaacggaaag gtacaatctc ccttttttta gtgttaacat cgctcaaccg
     9961 ggtggaaggt gagaggttaa tgtctttcat ttgtgtacct gattgcgaac caatttagca
    10021 tttgttggct agatggtttc gcaatgaact gttaataaac aaatttttct ttgtatgtga
    10081 tctttcgtgt gggtcaccac tgcaaataag gatataaaat gcctgttata actcttcctg
    10141 atggcagcca acgccattac gaccacgctg taagccccat ggatgttgcg ctggacattg
    10201 gtccaggtct ggcgaaagcc tgtatcgcag ggcgcgttaa tggcgaactg gttgatgctt
    10261 gcgatctgat tgaaaacgac gcacaactgt ccatcattac cgccaaagac gaagacggtc
    10321 tggagatcat tcgtcactcc tgtgcgcacc tgttagggca cgcgattaaa caactttggc
    10381 cgcataccaa aatggcaatc ggcccggtta ttgacaacgg tttttattac gacgttgatc
    10441 ttgaccgcac gttaacccag gaagatgtcg aagcactcga gaagcggatg catgagcttg
    10501 ctgagaaaaa ctacgacgtc attaagaaga aagtcagctg gcacgaagcg cgtgaaacat
    10561 tcgccaaccg tggggagagc tacaaagtct ccattcttga cgaaaacatc gcccatgatg
    10621 acaagccagg tctgtacttc catgaagaat atgtcgatat gtgccgcggt ccgcacgtac
    10681 cgaacatgcg tttctgccat catttcaaac taatgaaaac ggcaggggct tactggcgtg
    10741 gcgacagcaa caacaaaatg ttgcaacgta tttacggtac ggcgtgggca gacaaaaaag
    10801 cgcttaacgc ttacctgcag cgcctggaag aagccgcgaa acgcgaccac cgtaaaatcg
    10861 gtaaacagct cgacctgtac catatgcagg aagaagcgcc gggtatggta ttctggcaca
    10921 acgacggctg gaccatcttc cgtgaactgg aagtgtttgt tcgttctaaa ctgaaagagt
    10981 accagtatca ggaagttaaa ggtccgttca tgatggaccg tgtcctgtgg gaaaaaaccg
    11041 gtcactggga caactacaaa gatgcaatgt tcaccacgtc ttctgagaac cgtgaatact
    11101 gcattaagcc gatgaactgc ccgggtcacg tacaaatttt caaccagggg ctgaagtctt
    11161 atcgcgatct gccgctgcgt atggccgagt ttggtagctg ccaccgtaat gagccgtcag
    11221 gttcgctgca tggcctgatg cgcgtgcgtg gatttaccca ggatgacgcg catatcttct
    11281 gtactgaaga acaaattcgc gatgaagtta acggatgtat ccgtttagtc tatgatatgt
    11341 acagcacttt tggcttcgag aagatcgtcg tcaaactctc cactcgtcct gaaaaacgta
    11401 ttggcagcga cgaaatgtgg gatcgtgctg aggcggacct ggcggttgcg ctggaagaaa
    11461 acaacatccc gtttgaatat caactgggtg aaggcgcttt ctacggtccg aaaattgaat
    11521 ttaccctgta tgactgcctc gatcgtgcat ggcagtgcgg tacagtacag ctggacttct
    11581 ctttgccttc tcgtctgagc gcttcttatg taggcgaaga caatgaacgt aaagtaccgg
    11641 taatgattca ccgcgcaatt ctggggtcga tggaacgttt catcggtatc ctgaccgaag
    11701 aattcgctgg tttcttcccg acctggcttg cgccagttca ggttgttatc atgaatatta
    11761 ccgattcaca gtctgaatac gttaacgaat tgacgcaaaa actatcaaat gcgggcattc
    11821 gtgttaaagc agacttgaga aatgagaaga ttggctttaa aatccgcgag cacactttgc
    11881 gtcgcgtccc atatatgctg gtctgtggtg ataaagaggt ggaatcaggc aaagttgccg
    11941 ttcgcacccg ccgtggtaaa gacctgggaa gcatggacgt aaatgaagtg atcgagaagc
    12001 tgcaacaaga gattcgcagc cgcagtctta aacaattgga ggaataaggt attaaaggcg
    12061 gaaaacgagt tcaaacggcg cgccctaacc gtatcaatgg cgaaattcgc gcccaggaag
    12121 ttcgcttaac aggtctggaa ggcgagcagc ttggtattgt gagtctgaga gaagctctgg
    12181 agaaagcaga agaagccgga gtagacttag tcgagatcag ccctaacgcc gagccgccgg
    12241 tttgtcgtat aatggattac ggcaaattcc tctatgaaaa gagcaagtct tctaaggaac
    12301 agaagaaaaa gcaaaaagtt atccaggtta aggaaattaa attccgtcct ggtacagatg
    12361 aaggcgacta tcaggtaaaa ctccgcagcc tgattcgctt tctcgaagag ggtgataaag
    12421 ccaaaatcac gctgcgtttc cgcggtcgtg agatggcgca ccagcaaatc ggtatggaag
    12481 tgcttaatcg cgtgaaagac gatttgcaag aactggcagt ggtcgaatcc ttcccaacga
    12541 agatcgaagg ccgccagatg atcatggtgc tcgctcctaa gaagaaacag taaggccttc
    12601 aagtagcact gtctgtggag ccttcgggct tcacaggctt tgttcgcctt tgtttcgttt
    12661 attaacaatg cgaagtggaa gttattaaaa tgccaaaaat taagaccgta cgcggtgctg
    12721 ctaagcgctt caaaaaaacc ggtaaaggtg gttttaagca caagcacgct aacctgcgtc
    12781 acattctgac caaaaaagcg accaaacgta aacgtcacct gcgtccaaaa gccatggttt
    12841 ccaaaggcga tctgggcctg gtaatcgcgt gcctgccgta cgcataagcc gttaacgttt
    12901 ttaacttttt aattagaata tagatacagg agagcacata tggctcgcgt aaaacgtggt
    12961 gttattgcac gcgcacgtca caagaaaatt ttgaaacaag ctaaaggcta ctacggtgcg
    13021 cgttctcgcg tataccgcgt tgccttccag gctgttatca aagctggtca gtatgcttac
    13081 cgtgaccgtc gtcaacgtaa gcgtcagttc cgtcaactgt ggattgcgcg tatcaacgca
    13141 gcagcacgtc agaacggtat ttcttacagc aaattcatca atggcctgaa aaaagcctct
    13201 gttgaaatcg accgtaagat cctggctgat atcgcagtat tcgacaaagt agcgttcacc
    13261 gctctggttg aaaaagcgaa agcagctctg gcataagcca gttgaaagag ggagctagtc
    13321 tccctctttt cgtttcaacg ccatcaaaac attgactttt atcgccgtag ccttttcaat
    13381 aaaggtcttt tgaagagtac caaaaggtaa cgcaagcaat gaatgctgct attttccgct
    13441 tcttttttta ctttagcacc tgaatccagg aggctagcgc gtgagaagag aaacggaaaa
    13501 cagcgcctga aagcctccca gtggaggctt tttttgtatg cgcgttttga aaaataaaaa
    13561 gcctgtctca tcaggtgtaa ttgtcgcagc cagtctggac acggccagcg cggagaaacc
    13621 ggagcgtatt taagtacgtg agaatttcga gcactgcccg ggaccaaaat ggcaagtaaa
    13681 atagcctgat gggataggct ctaagtccaa cgaaccagtg tcaccactga cacaatgagg
    13741 aaaaccatgt cacatctcgc agaactggtt gccagtgcga aggcggccat tagccaggcg
    13801 tcagatgttg ccgcgttaga taatgtgcgc gtcgaatatt tgggtaaaaa agggcactta
    13861 acccttcaga tgacgaccct gcgtgagctg ccgccagaag agcgtccggc agctggtgcg
    13921 gttatcaacg aagcgaaaga gcaggttcag caggcgctga atgcgcgtaa agcggaactg
    13981 gaaagcgctg cactgaatgc gcgtctggcg gcggaaacga ttgatgtctc cctgccaggt
    14041 cgtcgcattg aaaacggcgg tctgcatccg gttacccgta ccatcgaccg tatcgaaagt
    14101 ttcttcggtg agcttggctt taccgtggca accgggccgg aaatcgaaga cgattatcat
    14161 aacttcgatg ctctgaacat tcctggtcac cacccggcgc gcgctgacca cgacactttc
    14221 tggtttgaca ctacccgcct gctgcgtacc cagacctctg gcgtacagat ccgcaccatg
    14281 aaagcccagc agccaccgat tcgtatcatc gcgcctggcc gcgtttatcg taacgactac
    14341 gaccagactc acacgccgat gttccatcag atggaaggtc tgattgttga taccaacatc
    14401 agctttacca acctgaaagg cactctgcac gatttcctgc gtaacttctt tgaggaagat
    14461 ttgcagattc gcttccgtcc ttcctacttc ccgttcaccg agccttctgc agaagttgac
    14521 gtcatgggta aaaacggtaa atggctggaa gtgctgggtt gcgggatggt gcatccgaac
    14581 gtgctgcgta acgttggcat cgacccggaa gtttactctg gtttcgcctt cgggatgggg
    14641 atggagcgtc tgactatgtt gcgttacggc gtcaccgacc tgcgttcatt cttcgaaaac
    14701 gatctgcgtt tcctcaaaca gtttaaataa ggcaggaata gattatgaaa ttcagtgaac
    14761 tgtggttacg cgaatgggtg aacccggcga ttgatagcga tgcgctggcg aatcaaatca
    14821 ctatggcggg cctggaagtt gacggtgtag aaccggttgc cggtagcttc cacggcgtgg
    14881 tcgttggtga agtggttgag tgtgcgcagc atccgaacgc tgacaaactg cgtgtgacaa
    14941 aagtgaatgt tggcggcgat cgcctgctgg acatcgtctg cggtgcgcca aactgccgtc
    15001 agggcctgcg tgtggcggta gctaccattg gtgctgttct gccgggtgat ttcaaaatta
    15061 aagcggcgaa actgcgtggt gaaccgtctg aagggatgct gtgctccttt tctgagctgg
    15121 gaatttctga cgatcatagc ggcattatcg agctgcctgc ggatgcgccg attggcaccg
    15181 acatccgtga atacctgaaa cttgatgaca acaccatcga aatcagcgtg acgccaaacc
    15241 gtgccgactg tttaggtatc attggtgttg cgcgtgacgt taccgtgctg aatcagctgc
    15301 cgctggttga accggaaatc gttccggttg gtgcgaccat cgacgacacg ctgccgatta
    15361 cagtcgaagc gccggaagcc tgcccgcgtt atcttggccg tgtggtaaaa ggcattaacg
    15421 ttaaagcgcc aactccgctg tggatgaaag aaaaactgcg tcgttgcggg atccgttcta
    15481 tcgatgcagt tgttgacgtc accaactatg tactgctcga actgggccag ccgatgcacg
    15541 ctttcgataa agatcgcatt gaaggcggca ttgtggtgcg gatggcgaaa gagggcgaaa
    15601 cgttggtact gcttgatggc actgaagcga agctgaatgc tgacactctg gtcatcgccg
    15661 accacaacaa ggcgctggcg atgggcggca tctttggtgg cgaacactct ggcgtgaatg
    15721 acgaaacgca aaacgtcttg ctggaatgcg ctttcttcag cccgctgtct atcaccggtc
    15781 gtgctcgtcg tcatggcctg catactgatg cgtctcaccg ttatgagcgt ggcgttgatc
    15841 cggcactgca gcacaaagcg atggaacgtg cgacccgtct actgatcgac atctgcggtg
    15901 gtgaggctgg tccggtaatt gatatcacca acgaagcaac gctgccgaag cgtgcaacca
    15961 tcactttacg tcgtagcaaa ctggatcgcc tgatcggcca tcatattgcg gatgagcagg
    16021 taactgacat tctgcgtcgt ctcggctgcg aagtgaccga aggcaaagac gagtggcagg
    16081 cagttgcgcc gagctggcgt ttcgatatgg agattgaaga agatctggtc gaagaagtcg
    16141 cgcgtgttta cggctacaac aacatcccgg atgagccggt acaggcaagc ctgattatgg
    16201 gtactcaccg tgaagctgac ctgtcgctca agcgcgtgaa aacgctgctc aacgacaaag
    16261 gctatcagga agtgatcacc tacagcttcg ttgatccgaa agtgcagcag atgatccatc
    16321 caggcgttga agccttactg ctgccaagcc cgatctctgt tgaaatgtca gcaatgcgtc
    16381 tttctctgtg gaccggcctg ctggcaaccg tggtgtacaa ccagaaccgt cagcagaacc
    16441 gtgtgcgcat tttcgaaagc ggtctgcgtt tcgtaccaga tactcaggca ccgttgggca
    16501 ttcgtcagga tctgatgtta gccggtgtga tttgcggtaa ccgttacgaa gagcactgga
    16561 acctggcaaa agagaccgtt gatttctatg atttgaaagg cgatcttgaa tccgttctcg
    16621 acctgaccgg taaactgaat gaggttgagt tccgtgcaga agcgaatccg gcactgcatc
    16681 cggggcaatc cgcagcgatt tatctgaaag gtgaacgtat tggttttgtt ggggttgttc
    16741 atcctgaact ggaacgtaaa ctggatctta acggtcgcac tctggtgttc gaactggagt
    16801 ggaacaagct cgcagaccgc gtggtgcctc aggcgcgcga gatttctcgc ttcccggcga
    16861 accgtcgtga catcgcggtg gtggtcgcag aaaacgttcc cgcagcggat attttatccg
    16921 aatgtaagaa agttggcgta aatcaggtag ttggcgtaaa cttatttgac gtgtaccgcg
    16981 gtaagggtgt tgcggagggg tataagagcc tcgccataag cctgatcctg caagatacca
    17041 gccgtacact cgaagaagag gagattgccg ctaccgtcgc caaatgtgta gaggcattaa
    17101 aagagcgatt ccaggcatca ttgagggatt gaacctatgg cgcttacaaa agctgaaatg
    17161 tcagaatatc tgtttgataa gcttgggctt agcaagcggg atgccaaaga actggttgaa
    17221 ctgtttttcg aagagatccg tcgcgctctg gaaaacggcg aacaggtgaa actctctggt
    17281 tttggtaact tcgatctgcg tgataagaat caacgcccgg gacgtaaccc gaaaacgggc
    17341 gaggatattc ccattacagc acggcgcgtg gtgaccttca gacccgggca gaagttaaaa
    17401 agccgggtcg aaaacgcttc gcccaaagac gagtaatctg atctaactaa aaaggccgct
    17461 ctgcggcctt ttttcttttc actgtcgaag agtcaccgta aaatcaacgc catgacactt
    17521 cagcagaacg gataccatgc tgacacttgc ccgccaacaa cagcgacaaa atattcgctg
    17581 gttattatgc ctgtcagttt tgatgctgct ggcgcttctc ttaagccttt gcgccggtga
    17641 acaatggatc tcgccaggtg actggttttc tcctcgtggc gaactgttcg tctggcagat
    17701 tcgcctgcca cgtacgctgg ctgtattgct ggttggtgcg gcgctggcta tatccggcgc
    17761 agtaatgcag gcgttgtttg aaaatcctct ggcagaacct ggactacttg gcgtctctaa
    17821 cggcgcaggc gtagggctta tcgccgcggt attgcttggg caagggcaac tcccaaactg
    17881 ggcactaggg ctgtgtgcga ttgctggcgc gcttatcatc actttaatac tcttacgttt
    17941 cgcccgtcgt catctttcga ccagtcggtt attgctggct ggcgttgcat tagggattat
    18001 ctgtagcgca ctaatgacgt gggctatcta cttttccacc tctgttgatt tacgtcagct
    18061 gatgtactgg atgatgggcg gttttggcgg cgtagactgg cggcaaagct ggctgatgct
    18121 ggcattgatc cccgtgttgt tgtggatctg ttgtcagtcc aggccgatga atatgttagc
    18181 acttggcgag atctcggcgc ggcaactggg tttacccctg tggttctggc gcaatgtgct
    18241 ggtggcagcg accggctgga tggttggcgt cagtgtggcg ctggcgggtg ctatcggctt
    18301 tattggtctg gtgatcccac atattcttcg gttgtgtggt ttaaccgatc atcgcgtatt
    18361 acttcccggc tgcgcgctgg caggggcgag cgcattgctg ctggccgatg ttgtagcgcg
    18421 cttggcatta gctgccgcag agctgcctat tggcgtggtc accgcaacgt tgggtgcgcc
    18481 ggtgtttatc tggttattgt taaaagcagg acgttagccg caaaaagacg gtctatgatt
    18541 aaaagctaag atttttactg accacaccca ggagaaacga tgcaagattc cattctgacg
    18601 accgtagtga aagatatcga cggtgaagtg accacgctgg agaagtacgc cggtaatgtg
    18661 ctgttgattg tcaatgtcgc ctcaaagtgt ggcttaacgc cgcaatatga gcagttggag
    18721 aatattcaga aagcctgggc cgatcgaggt tttgtggtgc tgggattccc gtgcaaccag
    18781 tttctggaac aagaaccggg cagcgatgaa gagattaaaa cttactgtac caccacatgg
    18841 ggggtgacgt tcccgatgtt cagtaagatt gaagttaatg gcgaaggacg ccatccgctg
    18901 tatcaaaaat tgattgccgc agcgccgacc gcagtcgcgc cggaagagag cggattctat
    18961 gcccgtatgg tcagcaaagg tcgtgcaccg ctgtaccctg atgatatttt atggaatttt
    19021 gagaaatttt tagttggcag ggacggattg gtcatccagc gtttttcccc ggatatgacg
    19081 ccggaagacc ccattgtgat ggaaagcatt aaactggcgt tggcaaaata atgtctattg
    19141 tgatgcagtt acaggatgtt gcggaatcta cccgcctggg gccactttct ggcgaggttc
    19201 gggctgggga gatcctgcac ctggtggggc cgaatggcgc gggtaagagt accttgcttg
    19261 cacgaatggc cggaatgacc agcggtaagg gaagcattca gttcgcgggg caacccctgg
    19321 aagcatggtc cgcaacaaaa ctcgcgctgc atcgcgccta tctttcacaa cagcagacgc
    19381 cgccgtttgc aatgccggtc tggcactacc tgacactgca tcagcacgat aaaacgcgta
    19441 ccgaactact gaatgatgtc gcaggggcgc tggctcttga tgacaaactc ggtcgtagca
    19501 ccaatcaact ttccggcggt gaatggcaac gcgtacgtct tgctgcggtg gtgctgcaaa
    19561 tcacaccaca agccaatccc gcaggtcaat tgctgcttct tgatgagccg atgaacagtc
    19621 ttgatgttgc gcaacaaagt gcgttagaca aaattctgag cgctctgtgt cagcaaggac
    19681 tggcgattgt gatgagcagt cacgatctca accacacatt gcgtcatgcg catcgggcgt
    19741 ggttgctaaa aggtggaaaa atgctggcca gtggacgcag agaagaggtg ctcacgccgc
    19801 caaatctggc gcaggcctat gggatgaatt ttcgccgtct ggatatcgaa ggtcacagaa
    19861 tgctgatttc gaccatctga tttcattagt atcttgaaaa tcgggtaatt acgacgctaa
    19921 attaatgcaa gaataaaaaa cagaggaacc gtacggaatg cgtttctgcc ttattttgat
    19981 cacagcactg tttctggccg ggtgtagcca ccataaagca ccgccgccaa atgccagact
    20041 ttctgattcg attaccgtta ttgccggttt gaacgaccag ctacaaagct ggcatggcac
    20101 gccgtatcgt tatggtggca tgacgcggcg cggtgtggac tgttcgggat ttgtggttgt
    20161 aacgatgcgc gatcgtttcg atttgcagct gccccgagaa accaaagaac aagcctctat
    20221 cggcacgcaa attgataaag acgagttgct gcctggtgac ctggtctttt tcaaaacggg
    20281 ttccggacaa aatggtttgc atgtaggtat ttacgatacc aacaaccaat ttatccacgc
    20341 ctctaccagc aagggagtga tgcgttcctc acttgataat gtctattggc agaaaaattt
    20401 ctggcaggcg agacgaattt agtatgtcga aaaatggacg gcgatgatac atcgccgtcc
    20461 atttactcct gaatacagta gtattttgca gacaaaaatt ccgtaaatgg attattcgtt
    20521 tataatcagg ataatctaa
//
LOCUS       DAZFQD010000094        20285 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.094, whole genome shotgun sequence.
ACCESSION   DAZFQD010000094 DAZFQD010000000
VERSION     DAZFQD010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20285
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.094"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            114..929
                     /gene="mepH"
                     /locus_tag="ACHKTW_003376"
     CDS             114..929
                     /gene="mepH"
                     /locus_tag="ACHKTW_003376"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HFO6464335.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKTAKKAASKSTTKSKTASSVKKSSITASKNAKTRSKHAVNKTASA
                     SFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPYRWGG
                     SSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAAPIERSELKNGDLVFFRTQG
                     RGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            1057..1638
                     /gene="sodB"
                     /locus_tag="ACHKTW_003377"
     CDS             1057..1638
                     /gene="sodB"
                     /locus_tag="ACHKTW_003377"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HFO6464336.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(1784..2959)
                     /locus_tag="ACHKTW_003378"
     CDS             complement(1784..2959)
                     /locus_tag="ACHKTW_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO6464337.1"
                     /translation="MKINYPLLALALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAA
                     GMLISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSR
                     ILTSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGW
                     RMSFLATAGLGVISMVSLFFSLPKGGAGARPEVKKELAVLMRPQVLSALLTTVLGAGA
                     MFTLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLL
                     LLMVIMLAIPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMRVASEAPGLSSSVNIG
                     AFNLGNALGAAAGGAVISAGLGYSFVPVMGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(3125..3214)
                     /gene="cydH"
                     /locus_tag="ACHKTW_003379"
                     /gene_synonym="ynhF"
     CDS             complement(3125..3214)
                     /gene="cydH"
                     /locus_tag="ACHKTW_003379"
                     /gene_synonym="ynhF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I accessory subunit CydH"
                     /protein_id="HFO6464338.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVAVGLTAALH"
     gene            3513..4538
                     /gene="purR"
                     /locus_tag="ACHKTW_003380"
     CDS             3513..4538
                     /gene="purR"
                     /locus_tag="ACHKTW_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HFO6464339.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            complement(4535..5467)
                     /gene="punR"
                     /locus_tag="ACHKTW_003381"
     CDS             complement(4535..5467)
                     /gene="punR"
                     /locus_tag="ACHKTW_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator PunR"
                     /protein_id="HFO6464340.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     VVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            5580..6791
                     /gene="punC"
                     /locus_tag="ACHKTW_003382"
     CDS             5580..6791
                     /gene="punC"
                     /locus_tag="ACHKTW_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside transporter PunC"
                     /protein_id="HFO6464341.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGLTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEVGCQNH
                     GNAEVAHSESH"
     gene            7082..8230
                     /gene="cfa"
                     /locus_tag="ACHKTW_003383"
     CDS             7082..8230
                     /gene="cfa"
                     /locus_tag="ACHKTW_003383"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HFO6464342.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIASAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCEGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(8270..8911)
                     /gene="ribE"
                     /locus_tag="ACHKTW_003384"
     CDS             complement(8270..8911)
                     /gene="ribE"
                     /locus_tag="ACHKTW_003384"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004746 - riboflavin synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HFO6464343.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            9126..10499
                     /gene="mdtK"
                     /locus_tag="ACHKTW_003385"
     CDS             9126..10499
                     /gene="mdtK"
                     /locus_tag="ACHKTW_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HFO6464344.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRSMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPDPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRLPSVI
                     ILQRASR"
     gene            complement(10540..11796)
                     /locus_tag="ACHKTW_003386"
     CDS             complement(10540..11796)
                     /locus_tag="ACHKTW_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HFO6464345.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVIGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPGGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGADLQQSTITVQQGGVLILDGSTVKGDGVTFIVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVTGDIHVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            12104..12180
                     /locus_tag="ACHKTW_003387"
     tRNA            12104..12180
                     /locus_tag="ACHKTW_003387"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12138..12140,aa:Val,seq:gac)
     gene            12185..12261
                     /locus_tag="ACHKTW_003388"
     tRNA            12185..12261
                     /locus_tag="ACHKTW_003388"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12219..12221,aa:Val,seq:gac)
     gene            12369..12674
                     /locus_tag="ACHKTW_003389"
     CDS             12369..12674
                     /locus_tag="ACHKTW_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HFO6464346.1"
                     /translation="MATLLQLHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTVRLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            12800..14404
                     /locus_tag="ACHKTW_003390"
     CDS             12800..14404
                     /locus_tag="ACHKTW_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
                     /protein_id="HFO6464347.1"
                     /translation="MKKIAIVGAGPTGIYTLFSLLQQQTPLSISIFEQADEAGVGMPY
                     SDEENSKMMLANIASIEIPPINCTYLEWLQKQEASHLQRYSVKKETLHDRQFLPRILL
                     GEYFRDQFLRLVDQARQQKFAVAVYESCQVTDLQITNAGVMLATNQDLPSETFDLAVI
                     ATGHVWPDEEEATRTYFPSPWSGLMEAKVDACNVGIMGTSLSGLDAAMAVAIQHGSFI
                     EDDKQHVVFNRDNASEKLNITLMSRTGILPEADFYCPIPYEPLHIVTDQALNAEIQKG
                     EEGLLDRVFRLIVEEIKFADPDWSQRIALESLNVDSFAQAWFAERKQRDPFDWAEKNL
                     QEVERNKREKHTVPWRYVILRLHEAVQEIVPHLNEHDHKRFSKGLARVFIDNYAAIPS
                     ESIRRLLALREAGIIHILALGEDYKMEINESRTVLKTEDNSYSFDVFIDARGQRPLKV
                     KDIPFPGLREQLQKTGDEIPDVGEDYTLQQPEDIRGRVAFGALPWLMHDQPFVQGLTA
                     CAEIGEAMARAVVKPASRARRRLSFD"
     gene            complement(14416..15228)
                     /gene="ydhT"
                     /locus_tag="ACHKTW_003391"
     CDS             complement(14416..15228)
                     /gene="ydhT"
                     /locus_tag="ACHKTW_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdhT"
                     /protein_id="HFO6464348.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIHHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGEIPFCSQFARSDDHARIGCCED
                     NARIATAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGPGGSAALAYHDGERVRFAVAIEGENNIRTGVRYRLNEQHQFVEC"
     gene            complement(15232..16017)
                     /gene="phsC"
                     /locus_tag="ACHKTW_003392"
     CDS             complement(15232..16017)
                     /gene="phsC"
                     /locus_tag="ACHKTW_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HFO6464349.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFTPEFWPVWLIIAGVLLVGMWLVL
                     GLHALLRARGVKKSVTDYGEKIYLYCKAVRLWHWSNALLFVLLLASGLINHFALVGAT
                     AVKSLVAVHEVCGFLLLACWLGFVLINAVGGNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMYGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            complement(16014..16682)
                     /locus_tag="ACHKTW_003393"
     CDS             complement(16014..16682)
                     /locus_tag="ACHKTW_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFO6464350.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYQVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQQNKYYQYQLPGAGKPHLYRRFGQHLIK
                     KENV"
     gene            complement(16746..17384)
                     /locus_tag="ACHKTW_003394"
     CDS             complement(16746..17384)
                     /locus_tag="ACHKTW_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HFO6464351.1"
                     /translation="MNHRDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSAAGQLVARAVFLSPPYSVAEEELSVLLESIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDVAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            complement(17397..19499)
                     /locus_tag="ACHKTW_003395"
     CDS             complement(17397..19499)
                     /locus_tag="ACHKTW_003395"
                     /EC_number="1.2.7.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
                     /protein_id="HFO6464352.1"
                     /translation="MANGWTGNILRVNLTTGNITLEDSSKFKSFVGGMGFGYKIMYDE
                     VPPGTKPFDEANKLVFATGPLTGSGAPCSSRVNITSLSTFTKGNLVVDAHMGGFFAAQ
                     MKFAGYDVIIIEGKAKSPVWLKIKDDKVSLEKADFLWGKGTRATTEEICRLTSPETCV
                     AAIGQAGENLVPLSGMLNSRNHSGGAGTGAIMGSKNLKAIAVEGTKGVNIADRQEMKR
                     LNDYMMTELIGANNNHVVPSTPQSWAEYSDPKSRWTARKGLFWGAAEGGPIETGEIPP
                     GNQNTVGFRTYKSVFDLGPAAEKYTVKMSGCHSCPIRCMTQMNIPRVKEFGVPSTGGN
                     TCVANFVHTTIFPNGPKDFEDKDDGRVIGNLVGLNLFDDYGLWCNYGQLHRDFTYCYS
                     KGVFKRVLPAEEYAEIRWDQLEAGDVNFIKDFYYRLAHRVGELSHLADGSYAIAERWN
                     LGEEYWGYAKNKLWSPFGYPVHHANEASAQVGSIVNCMFNRDCMTHTHINFIGSGLPL
                     KLQREVAKELFGSEDAYDETKNYTPINDAKIKYAKWSLLRVCLHNAVTLCNWVWPMTV
                     SPLKSRNYRGDLALEAKFFKAITGEEMTQEKLDLAAERIFTLHRAYTVKLMQTKDMRN
                     EHDLICSWVFDKDPQIPVFTEGTDKMDRDDMHASLTMFYKEMGWDPQLGCPTRETLQR
                     LGLEDIAADLAAHNLLPV"
     gene            complement(19520..20146)
                     /locus_tag="ACHKTW_003396"
     CDS             complement(19520..20146)
                     /locus_tag="ACHKTW_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HFO6464353.1"
                     /translation="MNPVDRPLLDIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
BASE COUNT         5053 a         5304 c         4751 g         5177 t
ORIGIN      
        1 gtatttttta acaataaaag ctattaactt tctcttcttc tatgcattag aatcatcaag
       61 ttttgtaaat cagacgcagg catgatagac ctgccttaac agagggacgc tcagtggcgc
      121 ggataaaccg tatttcgatc acgctctgtg ctttactttt taccaccctg cctttaacgc
      181 ctatggccca tgcttcaaag caagccaggg agagttctgc taccactcat atcaccaaaa
      241 aagcagataa aaagaaaagc acggcaacca ccaaaaagac ccagaaaaca gcgaaaaaag
      301 ccgccagtaa aagtacgacc aaaagcaaaa ccgcttcttc cgttaaaaaa tcttccatta
      361 ccgcttctaa aaacgccaaa actcgcagca aacacgccgt caataaaacg gcctcagcca
      421 gctttaccga aaagtgtacc aaacgtaagg gctataaatc gcattgtgtg aaagtcaaaa
      481 atgccgcgtc aggaactctt gccgacgcgc acaaagcgaa ggtgcaaaaa gctacgaaag
      541 tggcaatgaa taaactgatg cagcaaattg gtaagccata tcgttggggt ggcagctcac
      601 cgcgtaccgg ttttgattgc agcggcctgg tttattacgc ttataaagat ttggtgaaaa
      661 ttcgtattcc gcgtacggcg aatgaaatgt atcacctgcg tgatgcagcg ccaatcgaac
      721 gtagtgaact gaaaaacggc gacctggtct ttttccgtac tcagggacgc ggcacagccg
      781 atcatgtcgg cgtgtatgtc ggcaacggca aatttattca gtcaccgcgc acaggtcagg
      841 aaattcaaat cacttctctc agtgaagact actggcagcg ccactatgtt ggcgctcgtc
      901 gggtaatgac cccaaaaaca cttcgctaaa actttaccct gttgttacgg caacagggta
      961 agttcatctt ttgtctcacc ttttaatttg ctaccctatc catacgcaca ataaggctat
     1021 tgtacgtatg caaattaata ataaaggaga gtagcaatgt cattcgaatt acctgcacta
     1081 ccatatgcta aagatgctct ggcaccgcac atttctgcgg aaactatcga gtatcactac
     1141 ggcaagcatc atcagactta tgtcactaac ctgaacaacc tgattaaagg taccgcgttt
     1201 gaaggtaaat cactggaaga gattattcgc agctctgaag gtggcgtatt caacaacgca
     1261 gctcaggtct ggaaccatac tttctactgg aactgcctgg caccgaacgc cggtggcgaa
     1321 ccgactggaa aagtcgctga agctatcgcc gcatcttttg gcagctttgc cgatttcaaa
     1381 gcgcagttta ctgatgcagc gatcaaaaac tttggttctg gctggacctg gctggtgaaa
     1441 aacagcgatg gcaaactggc tatcgtttca acctctaacg cgggtactcc gctgaccacc
     1501 gatgcgactc cgctgctgac cgttgatgtc tgggaacacg cttattacat cgactatcgc
     1561 aatgcacgtc ctggctatct ggagcacttc tgggcgctgg tgaactggga attcgtagcg
     1621 aaaaatctcg ctgcataata actgatggca aatgcagcat tgcctgatgc gcttcgctta
     1681 ccatgccaac gcggatgagg cgttaacagc gcattcggca agataaatcg cacgttgtca
     1741 gcaactgtaa cgcagaagct tatccttctg cgtttttgtt taattagctg ttagcaacgc
     1801 aaactgtttc aggttgtttt ctggctgaca taaacaccag caataatgcc agtcccgcga
     1861 caatcgctcc catcaccggc acaaagctgt atcccagccc agcggaaatt accgcaccac
     1921 cagcagctgc tcccagcgca tttccaagat taaaggcacc aatattgact gatgaagaca
     1981 gacccggcgc ttcactggcg acacgcatca cgcgcatctg taacggcggt acgaccgcaa
     2041 aggttgctgc gccccacacc accatgctaa tagctgcgcc gaactcattg cgggccagga
     2101 acgggattgc cagcataatc accatcagca gcaacaaaaa gcctttcaac gtgccgttaa
     2161 ctgaacgatc tgccagtttg ccgccgagat agttaccgat agagaatccg acaccaatca
     2221 gcaccagcat tgccgtgacg aacactggtg ttgcgtgggt aatactttgt agtaccggag
     2281 agatataggt gtagagagta aacattgcac cagctcccag taccgtcgtc agcaatgcag
     2341 acagcacctg cggacgcatt aataccgcca gctctttttt cacttcaggt cgtgcccctg
     2401 caccaccttt aggtaatgag aagaacagac ttaccattga aatcactccc agccccgccg
     2461 ttgccagaaa tgacatccgc cagccgatgg tttcacccaa ccaggtcgcc gccggcacgc
     2521 caccgatatt tgccagggtt aaccccataa acatagtggc aactgcgctg gcctgtttat
     2581 gttttggcac cacgcttgcg gccacgactg aacccaaacc aaaaaatgct ccgtgattca
     2641 ggctggtcaa aatgcgtgaa agcatcaggg tcatataatc cggcgcgatg gcggaaagta
     2701 cgttgccgag cgtgaaaatt gccatcagga aaatcaacgc actgcggcgg gcacgatgag
     2761 aaagtagaag cgtcatcagc ggcgcgccaa ccattacgcc aactgcatag gcactgatta
     2821 acattccggc agcgggaatc gagacatcca caccgcgcgc aatgacgggc aacaagccca
     2881 ttggcgagaa ctccgttgtc ccgataccaa acgcgccaat cgccagcgcc agcgccagca
     2941 acggatagtt aattttcatg ccttatctcc acctcttcgc gtcattacgc gatattcatt
     3001 aaagtggcga aagcatgaca gcaatcacaa aaaaatgaaa ataacaaaaa gagaaaacac
     3061 ttttgccatt ttgctaacaa acaggaagga gatgcgaggg agaacgcgct ccctcgagtg
     3121 gaaatcagtg cagcgcggca gtcaaaccca cggccacgat caaaccgagg acgataatcg
     3181 ttgttaccag tgaaaattta aggtcggtgc tcatcaagtt ttctcctttt ttattaccac
     3241 acaaatagtg atattacgca tttttacaca ctgtgatgaa aaaatctccc gtcatttata
     3301 atgataagtg tttttaccac ttcccctttt cgtcaagatc ggccaaaatt ccacgcttac
     3361 actatttgcg tactggccat tgaccccttc ctgacgctcc gtgtcgtttt tccggcgtac
     3421 cgcaacactt ttgttgtgcg taaggtgtgt aaaggcaaac gtttaccttg cgattttgca
     3481 ggagctgaag ttagggtctg gagtgaaatg gaatggcaac aataaaagat gtagcgaaac
     3541 gagcaaacgt ttccactaca actgtgtcac acgtaatcaa caaaacacgt ttcgtcgctg
     3601 aagaaacgcg caacgccgtg tgggcagcga ttaaagaatt acactactcc cctagcgcgg
     3661 tggcgcgtag cctgaaggtt aaccacacca agtctatcgg tttgctggcg accagcagcg
     3721 aagcggccta ttttgccgag atcattgaag cggttgaaaa aaattgcttc cagaaaggtt
     3781 acaccctgat tctgggtaat gcgtggaaca atcttgagaa acagcgggct tatctgtcga
     3841 tgatggcgca aaaacgcgtc gatggtctgc tggtgatgtg ttctgagtac ccagagccgt
     3901 tgctggcgat gctggaagag tatcgccata tcccaatggt ggtgatggac tggggtgaag
     3961 caaaagctga cttcaccgat gcggtcattg ataacgcgtt cgaaggcggc tacatggccg
     4021 ggcgttatct gattgaacgc ggtcaccgcg aaatcggcgt catccccggc ccgctggaac
     4081 gtaacaccgg cgcaggccgc cttgccggtt ttatgaaggc gatggaagaa gcgatgatca
     4141 aggtgccgga aagctggatt gttcagggtg actttgaacc cgaatctggt tatcgcgcca
     4201 tgcagcaaat actgtcgcag ccgcatcgcc ctactgccgt cttctgtggt ggcgatatca
     4261 tggcaatggg cgcactttgt gctgctgatg aaatgggtct gcgcgtcccg caggatgttt
     4321 cgctgatcgg ttatgataac gtgcgcaacg cccgctattt tacgccggcg ctgaccacga
     4381 tccaccagcc aaaagattcg ctgggtgaaa cagcgttcaa catgctgttg gatcgtatcg
     4441 tcaacaaacg tgaagaaccg cagtccattg aagtgcatcc gcgcttgatt gaacgccgct
     4501 ccgtggctga cggcccgttc cgcgactatc gtcgttaatc acccgttgcg ggagtctctt
     4561 ccggctcccg cagccactcc ttattcagcg tctcactatc gccgagatac tcaagcagcc
     4621 aggttaacgc aggcgacata tcattttgct gccatgtcag acaacatgcc gaatccggga
     4681 aggggttttc caattcaagt gctacccact tcccctcatt aagccacggt ttggcgaaat
     4741 gtgttggcac catccctatg cataatcctg ccgagataca ggttgccgat gattcccagt
     4801 caggcacgac aactcttttt tggttatcca gcaaccaggt aatacgttta ggtagcgttc
     4861 gcgaggtgtc ttcgcgcacc aacgacggcc agttgcgcaa cgtatcatcg ctgaatgggc
     4921 catccatcaa cgccagcggg tggtggctgg caacaacgca actccagctt agcatcccca
     4981 tatcccggaa ggcataacga ccgcctaccg gaatcgcgcg tgttgcgcca atcgccagtt
     5041 ccacgcgccc gtcggaaagc gcatcccaga caccgttgaa cacttcctga aagacaagaa
     5101 gttcgacatc atcaaaatgg cgataaaaat caacgatcat ctgccgtgta cgttctggcc
     5161 tgacaatatt atccactgcg atagctaact gaccgcgcca gccgttcgct atctgctgac
     5221 attgctggcg ggtgatctgc atttttttga caacagagcg cccttctttg agaaaccacg
     5281 ctccagcagc ggtcagctcc acatcacggt gccgtcgttc aaagagcggc accgccagcc
     5341 actcttccag ctgacgcacg gtatagctga ccgcagaagg aacgcgatgc agctcctgtg
     5401 ccgcagcgct aaaactacca ttacgcgcta ccgcatcaac aacttcgagt gaatattctg
     5461 accacatagt ctgcctgcaa aatttttgaa accagtcatc aaatattacc gtttcacaac
     5521 actaatttca ctccctacac tttgcggcgg tgtttaattg agagatttag agaatataca
     5581 tgcaacctgg gaaaagattt ttagtctggc tggcgggttt gagcgtactc ggttttctgg
     5641 caaccgatat gtatctgcct gctttcgccg ccatacaggc cgacctgcaa acgcctgcgt
     5701 ctgctgtcag tgccagcctt agtctgttcc ttgccggttt tgccgcagcc cagcttctgt
     5761 gggggccact ctccgaccgt tatggtcgta aaccggtgtt attaatcggc ctgacaattt
     5821 ttgcgttagg tagtctgggg atgctgtggg tagaaaacgc cgctacgctg ctggtattgc
     5881 gttttgtaca ggctgtgggt gtctgcgccg cggcggttat ctggcaagcg ttagtgacgg
     5941 attattatcc ttcacagaaa gttaaccgaa tttttgcgac catcatgccg ctggtgggtc
     6001 tatctccggc cctggctcct ctgttaggaa gctggctgct ggtccatttt tcctggcagg
     6061 cgattttcgc caccctgttt gccattaccg tggtgctgat tctgcctatt ttctggctca
     6121 aacccacgac gaaggcccgt aacaatagtc aggatggtct gacctttacc gacctgctac
     6181 gttctaaaac ctatcgcggc aacgtgctga tatacgcggc ctgttcagcc agtttttttg
     6241 catggctgac cggttcaccg ttcatcctta gtgaaatggg ctacagcccg gcagttattg
     6301 gtttaagtta tgtcccgcaa actatcgcgt ttctgattgg tggttatggc tgtcgcgccg
     6361 cgctgcagaa atggcaaggc aagcagttat taccgtggtt gctggtgctg tttgcagtca
     6421 gcgtcattgc gacctgggct gcaggcttca ttagccatgt gtcgctggtc gaaatcctga
     6481 tcccattctg tgtgatggcg attgccaatg gcgcgatcta cccgattgtt gtcgcccagg
     6541 cgctgcgtcc cttcccacac gcaactggtc gcgccgcagc gttgcagaac actctacaac
     6601 tgggtctgtg cttcctcgca agtctggtag tttcctggct gatcagtatc agcacgccat
     6661 tgctcaccac caccagcgtg atgttatcaa cagtagtgct ggtcgctctg ggttacatga
     6721 tgcaacgttg tgaagaagtt ggctgccaga atcatggcaa tgccgaagtc gctcatagcg
     6781 aatcacactg atctatatcg atatacttat acttaggctg ctaacaaaat tttgttgtat
     6841 gattgaaatt agcggcctat actaatttcg agttgttaaa gctacgataa atattatgtt
     6901 tttacgggga caggatcgtt cccgactcac tatggatggt catttcggca aaggttcctc
     6961 ctttccctct gttctacgtc ggattataga ctcgcggttt tttctgcgag atttctcaca
     7021 aagcccaaaa agcgtctacg ctgttttaag gttctgatca ccgaccagtg atggagaaac
     7081 tatgagttca tcgtgtatag aagaagtcag tgtaccggat gacaactggt accgtatcgc
     7141 caacgaatta cttagccgtg ccggtatagc cattaacggt tctgccccgg cggatattcg
     7201 tgtgaaaaac cccgattttt ttaaacgcgt tctgcaagaa ggctctttgg ggttaggcga
     7261 aagttatatg gatggctggt gggaatgtga ccgactggat atgtttttta gcaaagtctt
     7321 acgcgcaggt ctcgagaacc aactccccca tcatttcaaa gacacgctgc gtattgccag
     7381 cgctcgtctc ttcaatctgc agagtaaaaa acgtgcctgg atagtcggca aggagcatta
     7441 cgatttgggt aatgacttgt tcagccgcat gcttgatccc ttcatgcaat attcctgcgc
     7501 ttactggaaa gatgccgata atctggaatc tgcccagcag gcgaagctca aaatgatttg
     7561 tgaaaaattg cagttaaaac cagggatgcg cgtactggat attggctgcg gctggggcgg
     7621 actggcgcac tacatggcat ctaactatga cgtaagcgtg gtcggcgtca ccatttctgc
     7681 cgaacagcaa aaaatggctc aggaacgctg tgaaggcctg gatgtcacca ttttgctgca
     7741 agattatcgt gacctgaacg accagtttga tcgtattgtt tctgtgggga tgttcgagca
     7801 cgtcggaccg aaaaattacg atacctattt tgcggtggtg gatcgtaatt tgaaaccgga
     7861 aggtatattc ctgctccata ctatcggttc gaaaaaaacc gatctgaatg ttgatccctg
     7921 gattaataaa tatatttttc cgaacggttg cctgccctct gtacgccaga ttgctcaatc
     7981 cagcgaaccc cacttcgtga tggaagactg gcataacttc ggtgctgatt acgatactac
     8041 gttgatggcg tggtatgaac gattcctcgc cgcatggcca gaaattgcgg ataactacag
     8101 tgaacgcttt aaacgaatgt ttacctatta tctgaatgcc tgtgcaggtg ctttccgcgc
     8161 ccgtgatatt cagctctggc aggtcgtgtt ctcacgcggt gttgaaaacg gccttcgagt
     8221 cgctcgctaa aggctattct atcgccccct ctccgggggc gatttcagat caggcttctg
     8281 tacctggttg attcatggca ttttctcgtg ccgccagcac acgttctacc gtatctacca
     8341 ctgcctgagt ttgtggatca atttcaatgt tgacgcgtgc gccgagtttt ttcttcccaa
     8401 gagtcgtgcg ttccagagtt tccggaatta aatggacgca aaaacgcgtt ggcgtgactt
     8461 cgccgacggt caggctaata ccgtcgatgc caataaatcc tttgtacagg atatatttca
     8521 tcaactgact atcctggact ttaaaccaga tctggcgatt attttccgag gttaatattt
     8581 tcgccacttc agcggtggtc ataatatgac ctgacattaa gtgtccgcca atttcatcac
     8641 tgaatttcgc cgcacgctca acgtttaccc aatcccccac ttttaaatcg ccaagattgg
     8701 taatgcgtaa cgtttctttc atcaggtcaa aactgacatg gttgccgtta atttccgtca
     8761 cggtcaggca gcaaccgtta tgcgccacgg aagcacctgt ttccaggccg tccagcatgt
     8821 ggtcgggtaa ctccaccaca tgcgtacgaa aatttggttt ctcgtcaatc gacaccagtt
     8881 ttgcggtgcc ctgtacaatc cccgtaaaca tacttacaac tcctgaaatc agttaagaca
     8941 ttctgttcag cacaatagca ggtggcaaac gcccttacca gtgaaggggt aagaatgact
     9001 attttttcac tggagaatta ataaatcctc gctacaatag actgaatttc ccctgcttct
     9061 tctttttgct gcccattcag gcggcttttt agtctctcat ataactacaa ataaaaggtg
     9121 ttcacgtgca gaagtatatc agtgaagcgc gtctgttatt agcattagca atcccggtga
     9181 ttctcgcgca aatcgcccaa actgcgatgg gttttgtcga taccgtgatg gcgggcggct
     9241 atagtgccac cgacatggcg gcggtcgcta tcggtacttc tatctggctt ccggcgatcc
     9301 tctttggtca cggactgctg ctggcattaa cgccggttat cgcgcaatta aatggttccg
     9361 gtcgacgtga gcgcattgcg catcaggtgc gacaaggttt ctggctggca ggttttgttt
     9421 ccgttctcat tatgcttgtg ctgtggaatg caggttacat tatccgctcc atggaaaaca
     9481 tcgatccggc tctggcggac aaagccgtgg gttatctgcg tgcgttgttg tggggcgcgc
     9541 cgggctatct gttcttccag gttgcccgta accaatgtga aggtctggca aaaaccaagc
     9601 cgggtatggt aatgggcttt atcggcctgc tggtgaacat tccggtgaac tatatcttta
     9661 tttatggtca tttcggtatg cctgagctcg gtggcgttgg ttgtggcgtg gctactgcgg
     9721 cggtgtattg ggtcatgttc cttgccatgg tttcttacat taaacgcgcc cgctccatgc
     9781 gcgatattcg taacgaaaaa ggcaccgcga aacccgatcc tgcggttatg aaacgactga
     9841 ttcaactcgg tttgccgatt gcgctggcac tattctttga agtgacactg tttgccgtcg
     9901 tggctctgtt agtgtctccg ctcggtattg ttgatgtcgc aggacaccag attgccctga
     9961 actttagttc actgatgttt gtgcttccaa tgtcgctggc ggcagcggta actatccgcg
    10021 taggttatcg tctgggtcag ggctcaacgc tggatgcgca aaccgctgcg cggaccgggc
    10081 ttatggtggg tgtctgtatg gcaaccctga cggccatatt cacggtttca ctgcgggagc
    10141 aaatcgccct gttgtacaac gacaatcccg aggttgtaac gctggctgcg catttgatgt
    10201 tgctggcggc ggtatatcag atttctgact caatccaggt gattggcagt gggattttgc
    10261 gtggttataa agatacgcgt tccattttct atatcacctt tacggcttac tgggtgctgg
    10321 gcttgccaag cggctatatt ctggcactga ccgatctggt cgttgaacct atggggccag
    10381 caggcttctg gataggcttt attattggcc tgacgtcggc agccattatg atgatgctgc
    10441 gtatgcggtt cctgcaacgt ctgccgtcag ttatcattct gcaacgagca tcccgctaat
    10501 aaagacaagg cgcaaccttc acgggttgcg cctgtgtttt tacgcaggct ggagcgttgc
    10561 gccaatcccg tcttcgtctg gctgtaattt cagagcgttg cacagagttt gccgactggc
    10621 atctgttatc tcaacgtgta tatccccggt aacttcccct ttcacattga tgaagtcagg
    10681 tgagatttct ttcgcactgg tttgcaggca aatcgctccc tctccgcgca ggcgtttcac
    10741 tttcagttgc acatgcgttg ccgcaccagt gatcagccac agttttccgc cattcagatt
    10801 gatgttacca acaataaaag tgacaccgtc accttttacc gtactgccgt cgagaatcaa
    10861 cacaccgccc tgctgtacag taatcgttga ttgttgcaga tctgcgccat agccaataat
    10921 cattgaagcg ccgtccagta atgcaacact cccggtcagt tcaaccggtg cgcgatcttc
    10981 ctctacgaca aaattttcgt ccgcgctggt gggtgacaac aggatcactg ttccaccttg
    11041 tattaccgca ttttccagcg taccggcatg cacttcagcc cgaccaccca cattcacgga
    11101 caggttttgc gtcttaccgg aactgtagag ctgaaggcca tccgtcccca gacgataaat
    11161 ggatccattc tcaacaatag tgtcagagtc ttcaccaccg agattattca tggtgccacc
    11221 agcaagcaac gtatcactgg ctttcccggc cagcataaaa acgccgccct gattaatctc
    11281 acaattgctg gcttccccac ctggatacac caacaattca ccaccttcat ctaccacgac
    11341 cgctgaggtt gtcccattaa ccaacaggcc gccctcttga tcgagaatga ttttttccgc
    11401 gcgatgtcct tccagtacac gcaggttacc gccattttcc agcaacaaac cgcaggcata
    11461 accctgatca acgctgaatt caccttcggg atggcggcca ttaaccgtag cgagcgtaga
    11521 gagactaatt gcgcctccct cggactgaac aatatctgtg gcaatggcac cgtcgctgac
    11581 cgatagcgtg ccattgttac cgtactgtgt gcatttggcg atcccaccgg ttgccacttt
    11641 caaccagcca gcccccgtga ccacggtatt ttcggctctt ccgccagctt caacaatcaa
    11701 ctcgccttca gtaagttgcg tcgcgccaat gacctcgccg gtcttaacaa tttgcacatt
    11761 cccgtcgctc atcaagtttt tcgcatcaga tcccatgatt tattcctttg ctgcatctgt
    11821 gtgcctttat tgctacctaa gtgtaaaggc tacggaggat ttatccacga cagatttgag
    11881 atggtggcaa acaactctgt ttaaactctg atacacgaat tattgggttg tatcagatgt
    11941 aaatgcgatc ctgaataaaa atcgcccttg caaatcaaca aaatatagcc aatttgctta
    12001 aaagctctgc aaacggtcga aatagcgcag aaaattacgt tttgcctctt gccaccttcc
    12061 cactctgccg ctaatattcg tccccgttgt cacctacaac gttgcgttca tagctcagtt
    12121 ggttagagca ccaccttgac atggtggggg tcgttggttc gagtccaatt gaacgcacca
    12181 tcctgcgtcc gtagctcagt tggttagagc accaccttga catggtgggg gtcggtggtt
    12241 cgagtccact cggacgcacc agattttctt aatctggtct tctccttttt ccctctgttt
    12301 cttctctgta tccaatacgt taaaagattt acactgtctt cgtatgcgtt atcagaagga
    12361 gaatcgctat ggcaactttg ttacaacttc attttgcttt taatggccca tttggcgacg
    12421 caatggctga gcagcttaaa ccgcttgctg agtcgattaa tcaggaacct ggttttctgt
    12481 ggaaggtatg gacagaaagt gaaaagaacc acgaagccgg tgggatctat ctttttactg
    12541 atgaaaaaag cgcccttgcc tatctggaaa aacatactgt ccgacttaaa aatctcggcg
    12601 ttgaggaagt tgtcgccaaa gttttcgatg tcaatgagcc actgagtcaa atcaatcagg
    12661 caaaactcgc ctgacagaat ttaatcaagg gcggttagcg cccttttcat ccctgtctga
    12721 aatttctcaa attctaaaaa tctcaaccaa acttatctga taacactaaa ttcgaaagaa
    12781 tgcgtacagg taagtaacaa tgaaaaaaat tgctattgtg ggtgccgggc ctacggggat
    12841 ctacacctta ttctcgcttc tacagcaaca aactccactt tctatttcta tcttcgagca
    12901 ggctgacgag gccggtgtcg ggatgccata cagtgatgag gaaaactcaa aaatgatgct
    12961 ggcaaatatt gccagtattg aaataccgcc gattaattgt acgtatctcg aatggctaca
    13021 aaagcaagaa gccagccatc tccagcgtta tagcgttaaa aaagaaacct tgcacgatcg
    13081 tcagttttta ccgcgaattc tgctgggcga atatttccgc gatcaatttt tacgattagt
    13141 agaccaggca cgacagcaaa aatttgcagt ggctgtttat gaatcatgcc aggttaccga
    13201 tctgcaaatt acaaatgctg gcgtcatgct cgctacaaat caggatttac ccagcgagac
    13261 gtttgattta gcggtgattg ccacgggtca cgtctggcct gatgaagaag aagcaaccag
    13321 aacgtatttt cccagtccgt ggtcaggcct gatggaagca aaggtcgatg cgtgtaacgt
    13381 gggtattatg ggaacatcct tgagcggact ggatgcggca atggcagtgg ctattcagca
    13441 tggttcgttc attgaagatg ataaacaaca cgtcgttttt aaccgcgata acgcaagtga
    13501 aaagctaaat attacgttga tgtcgcgcac gggtatttta cccgaagccg atttctattg
    13561 ccctattccc tacgagccct tacacatcgt caccgatcag gcattaaatg ctgagattca
    13621 aaaaggtgaa gagggccttt tggatcgggt atttagattg atagtagagg aaatcaagtt
    13681 tgctgatcca gactggagcc aacgcatagc cttagagagc ctgaatgtcg attcctttgc
    13741 tcaagcctgg tttgccgagc gcaaacaacg cgacccattt gactgggcag aaaaaaatct
    13801 ccaggaagtc gaacgcaata aacgagaaaa acatactgtt ccctggcgtt atgtcattct
    13861 gcgcctgcat gaagccgtac aggaaattgt tcctcatctg aatgaacacg accataaacg
    13921 gttcagtaaa ggccttgcca gggttttcat cgataattat gcggcaatcc cttcagagtc
    13981 tattcgtcgc ctacttgcct tacgtgaagc gggaatcatt catattctcg ccctcggtga
    14041 agactacaaa atggaaatta atgagtcgcg caccgtcctg aaaacggaag acaacagcta
    14101 ctcgtttgac gtttttattg atgcccgcgg acagcgtccg cttaaagtga aagatattcc
    14161 tttccctggg ctacgcgagc aattacagaa aacaggggat gaaatccctg atgttggcga
    14221 agattatacg ttacagcaac ccgaagatat tcgtggacgc gtagcgttcg gcgcgttgcc
    14281 ctggttgatg cacgaccagc ctttcgttca gggacttacg gcatgtgcag aaattggtga
    14341 ggcgatggct cgggcggtcg taaagcctgc atcccgtgct cgtcggcgtc tttcgtttga
    14401 ttaaaggtaa agctatcagc actcaacaaa ctgatgctgt tcattaagac gatagcggac
    14461 tccggttcga atattgtttt caccttcaat ggcgacggcg aagcgaactc tttcgccgtc
    14521 atgatatgcc agtgcggcgc ttccccctgg cccgagaata attgagtcga ccacgcccgt
    14581 gctcactacc gttgaatttt caccgctggc aatgatgtgt acattgtcgc cgctgttggc
    14641 aattctggcg tttgcgccaa agctggcgat ttgcaccagg tcaccattac tggcgacatg
    14701 acaccgttca cccaatgtac aaacgcgtac tcgcattccg gtattcgcaa tccgactact
    14761 atctccagcg ctgcttatcc gcgaagaatt accggttaca gcaacccgtg cgcgttcacc
    14821 ggagctgcca atgtgactgt taaagccgac acttccaatc cgcacgctat aacccatact
    14881 ggcaatttgc gcggcatacc cagcggttgc gattcgggcg ttatcctcac aacagccgat
    14941 gcgagcatga tcgtcgctgc gggcaaactg actgcaaaat ggtatctctc cggggtctgt
    15001 ggccagtttt tgcatagagg aaacttcctg atgcgcgaag ttttcatccg ccagccattt
    15061 tttccacgca tactcaacca gactctccgc ccagtccgta tatccttcct caatcagtgc
    15121 atgatgaata tcggcataac taccgccgcg tgggaaatgg cgcaggaacc agcgatacat
    15181 caccgctccg atacgccatt ctcgcaggtc tgctcgtgta atgatcattc gttagtgccg
    15241 gtgatagcca tcgaccatgc ttttaaaggt ttcgtgtggc gtacgccccg tggtgcaaag
    15301 ataaagatga ccgaagataa aaaagaggct tataaatgcc agagcaaaat gcgcctgcaa
    15361 taaccagtat cttacgccag gaaacacatc tcccacggct tgcggataga gacacagcag
    15421 ccccgtcaat agtaacaacg gcagcaatcc atacataaca ccaacatagg cgacctgctg
    15481 taagggatta aatttagact gggttgttgc cgggaaagga tgttcttccc cctgcataat
    15541 gccaaacaaa taaaatcgcg tttgttttgc cgctcgctcc agccacccct gacgacgaat
    15601 gcgatagtgg tgaccattac ccccaacggc attgatcagc acaaagccga gccagcatgc
    15661 gagtaacaaa aatccgcaaa cttcatgcac cgcaaccaga cttttaaccg cagttgcgcc
    15721 caccagcgca aagtgattta tcagcccact tgccagcaac aatacaaaga gtaacgcatt
    15781 cgaccagtgc cataatctga ccgctttgca gtaaagataa atcttctcac cataatcggt
    15841 gactgatttc ttcacgccac gagcacgaag caaggcatgc agccccagca ccaaccacat
    15901 cccaaccagc aatactccgg caatgatcaa ccacaccggc caaaattcgg gagtgaactg
    15961 tggcacatag ttcgctaact ggctctgaaa ctgttcagca tgttgcgacg ggttcataca
    16021 ttttcctttt taatcaaatg ttgaccgaac cggcgataca gatgcggttt cccggcacca
    16081 ggtagctgat attgatagta tttattttgc tgcaaccacg cctgaatttc tgggctatct
    16141 tcgcgaccaa agatcaatgc gtgttccggg caggcgctga cgcaaatggg cggaaagcct
    16201 tttgccagtc gggactcagc gcagaaatca catttatctg ccactttagt gacagggtta
    16261 aggtagcgaa cctggtacgg acaagcgcca atgcaataac tgcagccgat gcactgcgat
    16321 ttttctaccc gcacgatccc ctgctcatca cgccacgacg cccctgtcgg gcatacgtcg
    16381 atgcacggag catcttcgca gtgctgacat gactggcgaa aaaagtgata ctgcgtttcg
    16441 ttgtcgttat cggtgactgg aatgtgtgct atcgataaac ggcttccctg agcggggacg
    16501 tggttagttt tacggcatgc gcgggcgcaa atattacagc cattgcaccg tgactcgtca
    16561 tgaatcatgg cataccgaac ttccttttct tgcctcgtgt tcgctaacag agaagacgca
    16621 gaaccagtaa aaaagattac tgttcccatc cccagaacaa atttgcgtcg agtgaaggac
    16681 atctgattat cccctaatgc agtggctgca actgtccacc ctatccatga catctccctg
    16741 aaatttcagg ggttctgaaa ctgctctacc tcgaaccatt cgcataagcc ataagccttg
    16801 ccataggtca taaatcgctc gctaaacaag tacaacaccg ccgtcgatga ctccacctgc
    16861 cgtatatcgg catattcagg tgcaacatcc atcgcggcga tcgcggcttc aatctgcgct
    16921 tcctggaagt aatatggcgc ttgcatcagc atggcgactt tgtaaggacg aggatatgtc
    16981 tggcactcaa aacgaacggc gtgtgcaatg gcgcgacaaa tgtcctgctc cacaacttgc
    17041 aaagacatcg ccgcgtagtt ttcactcatg gcctgcgttg agtaatagta atcatcctgg
    17101 cttcccgtca tacaagcgat atccgcgtag tctccgttct gcttaatgct ttccagcaaa
    17161 actgacagct cctcttctgc aaccgaatag ggtggcgaga gaaacacggc gcgcgccacc
    17221 agctgcccag ccgcagaatg ctggcgaatg aactctgcca ggatttcagc cggttctggt
    17281 tcggttaccg tatcaacggg gtgacgcatc ccctgtaacc attgccgctt cgcctcgtca
    17341 acttcgctga ctttcgctaa cggcaattca tcccgatggt tcatctcacc cattccttac
    17401 acaggcagta gattgtgtgc tgccagatcg gcggcaatat cttccagccc cagacgctgc
    17461 aatgtttcgc gggtcggaca accaagctgt gggtcccagc ccatttcttt gtagaacatc
    17521 gtgagcgaag catgcatatc gtcacgatcc attttgtcag taccttcagt aaagaccggg
    17581 atctgcggat ccttgtcgaa tacccaggaa cagataagat cgtgttcatt acgcatatct
    17641 ttggtttgca tcagttttac cgtgtaggca cgatgcaacg taaagatacg ctctgcagct
    17701 aaatctaatt tttcctgagt catctcttcg ccggtgatcg ctttgaagaa tttggcttca
    17761 agcgccagat cgccccggta attacggctt ttcagcggcg aaacggtcat tggccagacc
    17821 cagttgcaca gggtgacggc gttatgcaaa cagacccgca acagcgacca ctttgcatat
    17881 ttaatttttg cgtcattgat tggcgtgtag tttttggttt catcgtaggc atcttcagaa
    17941 ccaaaaagct ctttcgccac ttcacgctgc agtttcaatg gcaaacccga accaataaaa
    18001 ttgatatggg tatgcgtcat gcagtcacgg ttgaacatac agttaacaat ggaaccgacc
    18061 tgcgctgacg cttcattggc atggtgaacc ggatagccaa acggcgacca gagtttattt
    18121 ttcgcgtagc cccagtactc ttcacccaaa ttccagcgtt ctgcgatggc atatgaacca
    18181 tcagccaggt gactcagctc acccacacga tgcgccagac ggtagtaaaa atctttaatg
    18241 aagttaacgt cacccgcttc cagttgatcc cagcgaattt ccgcatactc ttcagcaggc
    18301 agaacacgct tgaacacccc tttgctgtaa caataggtaa agtcgcgatg caactgcccg
    18361 tagttacacc ataggccgta gtcatcgaac aaattcagac ccaccaggtt accaatcaca
    18421 cggccatcgt ctttatcttc aaaatctttc ggaccgttcg ggaagatggt ggtatggaca
    18481 aagttggcta cacaagtgtt accacctgtg ctgggcacgc caaactcttt cacccgagga
    18541 atattcattt gggtcataca acggatcggg caagagtggc agccactcat ttttactgtg
    18601 tatttctctg ccgccggacc taagtcaaaa acggatttat aggtacgaaa gccgaccgtg
    18661 ttctgattgc ctggcggaat ttcccccgtt tcaatcggac caccttcagc cgcgccccaa
    18721 aacagccctt tacgtgctgt ccaacgtgac ttgggatctg aatactctgc ccacgattgc
    18781 ggcgtacttg gcacgacatg gttgttattc gcaccaataa gttcagtcat catgtaatcg
    18841 ttgagacgct tcatctcctg acgatcggca atgttgaccc ctttcgtccc ttcaaccgca
    18901 atcgctttca ggtttttcga acccataatt gcgccagttc ccgcaccgcc gctgtggtta
    18961 cggctattca acatgccaga gagaggaaca aggttttccc cagcctgacc aatagccgcc
    19021 acacaggttt ccggactggt caatcgacaa atttcttccg tcgttgcgcg cgtccctttt
    19081 ccccataaga aatcggcttt ttccaggcta actttgtcat ctttaatctt cagccatacc
    19141 ggtgatttcg ccttcccttc gataataatg acgtcgtatc cagcgaattt catttgcgct
    19201 gcaaaaaagc cacccatatg ggcatcgaca actaaatttc ctttggtaaa agtagaaagt
    19261 gaggtgatat ttacgcgaga actacagggg gcaccagatc cagttaatgg gccggtagca
    19321 aagactaatt tattcgcttc atcgaaaggt ttcgtgcctg gcggtacttc gtcatacata
    19381 attttgtagc cgaagcccat gccaccgaca aaacttttaa acttactgga atcttcgagg
    19441 gtaatatttc ctgtcgtgag attgactctt aatatattac ctgtccaacc gttagccatg
    19501 attttttcct ttgcaagatt cacacagtaa tatctttcca ctcgataatt tttaacgccc
    19561 ctgttgggca ggcgtttgcg cattcaccgc ataacacaca tttcgaggat tttttacttt
    19621 cggtatttac ggtggccatc atccacgggc atgccgtagt acaggcgcta cagccaatac
    19681 agcgtttatg atcgacggta atacagcctt ctttctgctg ccaggtaatc gcaccaatcg
    19741 ggcagacgtt catgcattgc ggctctttgc attgacggca ggtgtccgcg gtatagttga
    19801 gatcgccata caggccgccg ccagagccaa ccccgttgtc gccaaagaaa taattgcggt
    19861 ggattttgat acgggagaag aatgtcccta ctgagccatc gttgaagttg gtacaagaga
    19921 tttcacagcg gtggcaaccc gtgcatcgcg ctcgctgggt caccagcacc cctttgggtg
    19981 tatttatcaa ccctactgtg ccgctatcaa tatcttcctg tttgcaaccc aaaagtgaca
    20041 gtaacgcagg ggcaattgtt agaccagcaa ggccttttcc tgatatacgc aagaattcta
    20101 atcgcgttaa gccaatatct aatagtggac gatcaaccgg gttcatttat ttcgcatcct
    20161 cttcgcaaac acagtgctga caattcagca gcctgatatt aagcattcaa ctaatacgct
    20221 gctgactctg tccccaacac cagatatttt ccctaacccg atagtgtatg agaaaagatt
    20281 taacg
//
LOCUS       DAZFQD010000095        20180 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.095, whole genome shotgun sequence.
ACCESSION   DAZFQD010000095 DAZFQD010000000
VERSION     DAZFQD010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20180
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.095"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(79..1083)
                     /gene="argF"
                     /locus_tag="ACHKTW_003397"
     CDS             complement(79..1083)
                     /gene="argF"
                     /locus_tag="ACHKTW_003397"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFO6464354.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKAFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     "
     gene            1245..1661
                     /gene="rraB"
                     /locus_tag="ACHKTW_003398"
     CDS             1245..1661
                     /gene="rraB"
                     /locus_tag="ACHKTW_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HFO6464355.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            complement(1707..2210)
                     /gene="yjgM"
                     /locus_tag="ACHKTW_003399"
     CDS             complement(1707..2210)
                     /gene="yjgM"
                     /locus_tag="ACHKTW_003399"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO6464356.1"
                     /translation="MNNIAPQSPVMRRLTQQDNPAIARVIRQVSAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEFDGEVVGGGGIAPLTGSESDICELQKMYFLPAIRGK
                     GLAKKLALMAMEQAREMGFKRCYLETTAFLKEAIALYEHLGFEHINYALGCTGHVDCE
                     VRMLREL"
     gene            2403..3599
                     /gene="yjgN"
                     /locus_tag="ACHKTW_003400"
     CDS             2403..3599
                     /gene="yjgN"
                     /locus_tag="ACHKTW_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjgN family protein"
                     /protein_id="HFO6464357.1"
                     /translation="MAQVINEMDVPSHSFVFHGTGERYFLICVVNVLLTIITLGIYLP
                     WALMKCKRYLYANMEVNGQRFSYGITGGNVFVSCLVFVFCYFAILMTVSADMPLVGCV
                     LTLLLLVLLIFMAAKGLRYQALMTSLNGVRFSFNCSMKGFWWVTFFLPILMAIGMGTV
                     FFISTKMLHANSSSSVIISVVLMAIVGIVSIGIFNGTLYSLVMSFLWSNTSFGIHRFK
                     VKLDTTYCIKYAILAFLALLPFLAVAGYIIFDQILNAYDSSVYANDDIENLQQFMEMQ
                     RKMIIAQLIYYFGIAVSTSYLTVSLRNHFMSNLSLNDGRIRFRSTLTYHGMLYRMCAL
                     VVISGITGGLAYPLLKLWMIDWQAKNTYLLGDLDDLPLINKEEQPDKGFLARISRGIM
                     PSLPFL"
     gene            complement(3655..6510)
                     /gene="valS"
                     /locus_tag="ACHKTW_003401"
     CDS             complement(3655..6510)
                     /gene="valS"
                     /locus_tag="ACHKTW_003401"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFO6464358.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EDEVRKENNLGADVVLRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(6510..6953)
                     /gene="holC"
                     /locus_tag="ACHKTW_003402"
     CDS             complement(6510..6953)
                     /gene="holC"
                     /locus_tag="ACHKTW_003402"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HFO6464359.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGGAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            complement(7087..8598)
                     /gene="pepA"
                     /locus_tag="ACHKTW_003403"
     CDS             complement(7087..8598)
                     /gene="pepA"
                     /locus_tag="ACHKTW_003403"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HFO6464360.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            8752..8802
                     /gene="ytgA"
                     /locus_tag="ACHKTW_003404"
     CDS             8752..8802
                     /gene="ytgA"
                     /locus_tag="ACHKTW_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YtgA"
                     /protein_id="HFO6464361.1"
                     /translation="MSIIILATISTTQENK"
     gene            8865..9965
                     /gene="lptF"
                     /locus_tag="ACHKTW_003405"
     CDS             8865..9965
                     /gene="lptF"
                     /locus_tag="ACHKTW_003405"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HFO6464362.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            9965..11047
                     /gene="lptG"
                     /locus_tag="ACHKTW_003406"
     CDS             9965..11047
                     /gene="lptG"
                     /locus_tag="ACHKTW_003406"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HFO6464363.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEELGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTNLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(11166..12668)
                     /gene="yjgR"
                     /locus_tag="ACHKTW_003407"
     CDS             complement(11166..12668)
                     /gene="yjgR"
                     /locus_tag="ACHKTW_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like C-terminal domain-containing
                     protein"
                     /protein_id="HFO6464364.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLARLKNIGVNDWQPHANPVVVWDIFG
                     EKGHPVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGNNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAAHFFGEPMLDIKDWMRTDTNGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVLGQLGNRVQHALRAFTPKDQKAV
                     KAAAQTMRANPAFDTEKAIQELGTGEALISFLDVKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDEVDRESAYEMLQKGVQASAEQQNNPPAKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRRR"
     gene            complement(12746..13744)
                     /gene="idnR"
                     /locus_tag="ACHKTW_003408"
                     /gene_synonym="gntH"
                     /gene_synonym="yjgS"
     CDS             complement(12746..13744)
                     /gene="idnR"
                     /locus_tag="ACHKTW_003408"
                     /gene_synonym="gntH"
                     /gene_synonym="yjgS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator IdnR"
                     /protein_id="HFO6464365.1"
                     /translation="MRNHRISLQDIATLAGVTKMTVSRYIRSPKKVAKETGERIAKIM
                     EEINYIPNRAPGMLLNAQSYTLGILIPSFQNQLFADILAGIESVTSEHNYQTLIANYN
                     YDRDSEEESVINLLSYNIDGIILSEKYHTIRTVKFLRSATIPVVELMDVQGERLDMEV
                     GFDNRQAAFDMVCTMLEKRVRHKILYLGSKDDTRDEQRYQGYCDAMMLHNLSPLRMNP
                     RAISSIHLGMQLMRDALSANPDLDGVFCTNDDIAMGALLLCRERNLAVPEQISIAGFH
                     GLEIGRQMIPSLASVITPRFDIGRMAAQMLLSKIKNNDHNHNTVDLGYQIYQGNTL"
     gene            complement(13811..15130)
                     /gene="idnT"
                     /locus_tag="ACHKTW_003409"
     CDS             complement(13811..15130)
                     /gene="idnT"
                     /locus_tag="ACHKTW_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gnt-II system L-idonate transporter"
                     /protein_id="HFO6464366.1"
                     /translation="MPLIIIAAGVALLLILMIGFKVNGFIALVLVAAVVGFAEGMDAQ
                     AVLHSIQNGIGSTLGGLAMILGFGAMLGKLISDTGAAQRIATTLIATFGKKRVQWALV
                     ITGLVVGLAMFFEVGFVLLLPLVFTIVASSGLPLLYVGVPMVAALSVTHCFLPPHPGP
                     TAIATIFEANLGTTLLYGFIITIPTVIVAGPLFSKLLTRFEKAPPEGLFNPHLFSEEE
                     MPSFWNSIFAAVIPVILMAIAAVCEITLPKTNTVRLFFEFVGNPAVALFIAIVIAIFT
                     LGRRNGRTIEQIMDIIGDSIGAIAMIVFIIAGGGAFKQVLVDSGVGHYISHLMTGTTL
                     SPLLMCWTVAALLRIALGSATVAAITTAGVVLPIINVTHADPALMVLATGAGSVIASH
                     VNDPGFWLFKGYFNLTVGETLRTWTVMETLISIMGLLGVLAINAVLH"
     gene            complement(15195..15959)
                     /gene="idnO"
                     /locus_tag="ACHKTW_003410"
     CDS             complement(15195..15959)
                     /gene="idnO"
                     /locus_tag="ACHKTW_003410"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HFO6464367.1"
                     /translation="MNDLFSLAGKNILITGSAQGIGFLLATGLGKYGAQIIINDITAE
                     RAELAVEKLHQEGIQAVAAPFNVTHKHEIDAAVEHIEKDIGPIDVLVNNAGIQRRHPF
                     TEFPEQEWNDVIAVNQTAVFLVSQAVTRHMVERKAGKVINICSMQSELGRDTITPYAA
                     SKGAVKMLTRGMCVELARHNIQVNGIAPGYFKTEMTKALVEDEAFTAWLCKRTPAARW
                     GDPQELIGAAVFLSSKASDFVNGHLLFVDGGMLVAV"
     gene            complement(15983..17014)
                     /gene="idnD"
                     /locus_tag="ACHKTW_003411"
     CDS             complement(15983..17014)
                     /gene="idnD"
                     /locus_tag="ACHKTW_003411"
                     /EC_number="1.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-idonate 5-dehydrogenase"
                     /protein_id="HFO6464368.1"
                     /translation="MQVKTQSCVVAGKKTVAVTEQTIDWNNNGTLVQITRGGICGSDL
                     HYYQEGKVGNFMIKAPMVLGHEVIGKVIHSDSSELHEGQTVAINPSKPCGHCKYCIEH
                     NENQCTEMRFFGSAMYFPHVDGGFTRYKMVETSQCVPYPAKADEKVMAFAEPLAVAIH
                     AAHQAGELQGKRVFISGVGPIGCLIVSAVKTLGAAEIVCADVSPRSLSLGKEMGADVL
                     VNPQNDDMDHWKAEKGYFDVSFEVSGHPSSVNTCLEVTRARGVMVQVGMGGAMAEFPM
                     MTLIGKEISLRGSFRFTSEFNTAVSWLANGVINPLPLLSAEYPFTDLEEALRFAGDKT
                     QAAKVQLVF"
     gene            17231..17794
                     /gene="idnK"
                     /locus_tag="ACHKTW_003412"
     CDS             17231..17794
                     /gene="idnK"
                     /locus_tag="ACHKTW_003412"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFO6464369.1"
                     /translation="MAGESFILMGVSGSGKTLIGSKVAALLSAKFIDGDDLHPAKNID
                     KMSQGIPLSDEDRLPWLERLNDASYSLYKKNETGFIVCSSLKKHYRDILRKGSPHVHF
                     LWLDGDYETILARMQRRAGHFMPVALLKSQFEALERPQADEQDIVRIDINHDIANVTE
                     QCRQAVLAIRQNRICAKEGSASDQRCE"
     gene            complement(17798..18817)
                     /gene="ahr"
                     /locus_tag="ACHKTW_003413"
     CDS             complement(17798..18817)
                     /gene="ahr"
                     /locus_tag="ACHKTW_003413"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HFO6464370.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYDPGELKPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRVDWQWVIPLPENIDIKSAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQELLAMGADKVVN
                     SRDPQALKALAGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSVSGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIQHVRDGKARYRVV
                     LKADF"
     gene            19013..19097
                     /locus_tag="ACHKTW_003414"
     tRNA            19013..19097
                     /locus_tag="ACHKTW_003414"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19047..19049,aa:Leu,seq:caa)
     gene            19705..20004
                     /locus_tag="ACHKTW_003415"
     CDS             19705..20004
                     /locus_tag="ACHKTW_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6464371.1"
                     /translation="MAISAKKAAYKEKKAKAAELAKLRRQQKAALTRMIKKAREDGTF
                     DSLPDDIKAKCLKHEAKREKAAYFYNQWKEKQAYAARREEVAGIDELSLQHFKSI"
BASE COUNT         4932 a         5127 c         4949 g         5172 t
ORIGIN      
        1 taattgaact attactgcat aaaataatgt gatgccggga tgtttttgta tttcccggca
       61 tctttatagc gatatcaatt atttactgag cgtcgcgacc atcaccgctt tgatggtatg
      121 catgcggttt tccgcctgat caaaaacaat gctggcggca gattcgaaga cctcatcagt
      181 cacttccatt ccgccatgta ggccaaactc ttccgccatt ttcttaccaa gcgtcgtctg
      241 gtcgtcatga aacgcgggca ggcagtggag gaatttgacc tccgggttac cggtcaactg
      301 catcatcttg ctgttcacct gataatcacg cagtaatgca atccgttctg cccatttctc
      361 ttttgcttcc cccatcgaca cccatacatc ggtatagata aagtcagcac cttcaactcc
      421 tttggctaca tcttccgtca gcgtaatatt cccgccattt tgctgtgcca gggcgcggca
      481 ttccgtaacc agcgcagctt ccggccagca tgcctgtggc gcgaccagac gcaaatccag
      541 accggtaagc gccgcagctt cgagcatcga attgcccatg ttgttacgtg cgtcacctgc
      601 atagaccagc gtcatttcgt tgaatgcttt accgggtaaa tgctcctgca tggtgaggag
      661 atccgccagc aactgcgtgg ggtgaaactc attggtcagg ccgttccata ccggcacgcc
      721 agcgtattcc gccagtgttt cgacaatctc ctgaccatag ccgcgatact gaataccgtc
      781 atacatgcga cccagcacgc gggcagtgtc tttaatcgac tctttatgac caatctgact
      841 gccgcttggg ccgagataag taacgcgagc accctggtca tatgcggcaa cttcgaaaga
      901 gcatcgggta cgggtcgagt ctttttcgaa gatgagcgcg atgtttttac cagtgagttt
      961 ggcttcttct ttgccgcttt tcttatcggc tttcagcttc gcggctaact gcagcaggct
     1021 gttgagttca gctggcgtga aatcgagtaa tttcaggaaa tgcttatgat aaaacccgga
     1081 catagatccc tcctgtggtt aacgccttaa tgaattaaaa ttcaatttat atggatgatt
     1141 attcatttgc aagtctaaag cataaatctt tgtcacaaag gtggaggcaa tgtcagtggt
     1201 gtgtgacaat aagagtatcg gcaggacatt aagaggaatg agccatggca aacccggaac
     1261 aactggaaga acagcgtgaa gaaacacgtt tgattattga agaattactg gaagatggca
     1321 gcgatccgga cgcgctgtac accatcgaac accatctttc cgcagacgat ctggaaaccc
     1381 tggaaaaagc agcagttgaa gcgtttaaac tcggttacga agtgaccgat ccagaagagc
     1441 tggaagttga agatggtgat atcgtgattt gctgcgacat cctcagcgag tgcgcgttga
     1501 atgccgatct gatcgatgcc caggttgaac aactgatgac gctggcggag aaatttgacg
     1561 ttgagtacga cggttggggc acttactttg aagatcccaa tggcgaagac ggcgacgatg
     1621 aagattttgt cgatgaagac gatgacggtg ttcgccacta attaattgat taaaatgccc
     1681 gatagcacac actagcgggc atttttttag agttcgcgta gcatccgcac ttcacaatcg
     1741 acatggcccg tgcagccaag cgcatagttg atatgctcaa agcccaaatg ctcataaagc
     1801 gcaatggctt cctttaaaaa agcggtcgtt tccagatagc agcgtttgaa gcccatctct
     1861 cgtgcctgct ccatcgccat taaggccagt tttttcgcca gccctttacc gcgaatagcc
     1921 ggaagaaaat acatcttttg cagttcgcaa atgtccgatt cactcccggt taatggcgca
     1981 atcccaccgc cgccgaccac ttcaccatcg aactcaacca cccaatatgc atggccaggc
     2041 tgactataca cttgatacaa ctcgtcaagg ttcggatcgg cgacggtgta gcctttatca
     2101 gcggtaagac cgtattcggc ggatacctga cgaatgacgc gggcgatagc gggattatcc
     2161 tgctgcgtca ggcgacgcat tacaggtgat tgcggcgcaa tgttattcat agtttaaccc
     2221 aataaaaata aagtaattat acatttgttt aataccactc tcttcttacg tgcgcaagcg
     2281 acttataaag taataaagtt tttgccagcc tattactgat aagagtattg taacgtggca
     2341 gaacattttg ctgtctgtat atataaatta tatcaatgga ttttttaatt cagggaattt
     2401 ttatggctca agttattaat gaaatggatg ttccgtccca ttcgtttgtt tttcatggta
     2461 caggtgagag atattttctt atttgtgtgg tgaatgtgtt gttaacgatt ataacgctag
     2521 gtatctattt gccatgggca ttaatgaaat gtaagcgtta tctctatgct aatatggaag
     2581 ttaacggaca acgattttct tatggaatta ctggtgggaa tgtttttgtt agttgtcttg
     2641 tttttgtttt ttgctatttc gcaatcttaa tgacagtgtc agcagatatg ccacttgttg
     2701 gctgtgtttt gactttgtta ctgttggttt tgcttatatt tatggcagca aaaggactgc
     2761 gttatcaggc cttgatgacc agtctcaacg gcgtaagatt tagttttaat tgctctatga
     2821 aagggttctg gtgggtaacc tttttcttgc cgattttaat ggccattggg atggggactg
     2881 ttttctttat ctcgacaaag atgctacatg ccaatagttc aagtagtgtt attatatctg
     2941 tggttctgat ggcaatagtt ggtattgttt ccattggtat ttttaatggt actttatata
     3001 gcctggtaat gagttttctc tggagcaata ccagtttcgg tatacatcgt ttcaaggtga
     3061 aattagatac tacgtattgt ataaaatatg ccattctcgc atttttagct ttattacctt
     3121 ttctcgctgt tgctggttat attatcttcg atcaaatatt aaatgcatat gatagttctg
     3181 tgtatgcaaa tgatgatatt gagaatttac agcaatttat ggaaatgcaa cgtaaaatga
     3241 taatcgcgca gttaatctat tattttggga ttgctgttag caccagttat ttaacggtgt
     3301 cgttgcgaaa tcattttatg agcaacctgt cactgaatga tgggcgtatt cgttttcgct
     3361 caactttaac gtaccacggt atgctttatc gcatgtgtgc gttggtggtg atatccggga
     3421 ttacgggcgg tctggcttat ccactgctga aattatggat gattgactgg caggcaaaaa
     3481 atacgtattt gctgggcgat ttggatgacc ttcctttaat caataaagaa gaacaaccag
     3541 ataaaggctt cttagccagg atttcacggg gaattatgcc ttctttacca tttctgtaag
     3601 agataaaaaa ggccggagca tgctccggcc ttcgttttca ttactgtgtt ttgattacag
     3661 cgcggcgata acagcctgct gttcaatcag cttcgctttc gcttctgcat agccttccag
     3721 cttttcacgc tctttcgcga tgaccgcttc cggtgcgcgg gcgacaaagc cttcattcgc
     3781 cagcttgttc tcgatacggc tgatttcacc ttcaatcttc gccacttctt tcgccagacg
     3841 cgccagctca tcttctttgt tgatgaggcc agccatcggg atcagcagct ctgcaccgtc
     3901 gatgatctta gtaacggaaa ccggaccttt gtcatcggca ggcagcacgg tgatgctttc
     3961 cagacgcgcc agcgtttgca ggaagccacg gttttcattt acgcgacgtt ctgcatccgc
     4021 gctgcaacca cgcagtagca gctccagcgg tttgcccggc gcgatgttca tttctgcacg
     4081 gatgttacgt accgcaacga tcgcctgctt cagccactct gtgtcggcca gtgcggcttc
     4141 atcaacctga gatgcatcgt actgcgggaa cggctgcagc atgatggtgt cggcagtgat
     4201 accgcaaagt actttcacac gctgccagat ggtttcggtg atgaacggaa tgatcggatg
     4261 cgcgaggcgc agcagacctt ccagcacggt caccagcgta tggcgagtac cgcgcagttc
     4321 ggcttcggtg ccaccgttca ttaccggctt agtcagctcc agataccagt cacagaactg
     4381 gttccaggtg aactcataca gaatgcctgc ggcgatatcg aagcggaagc tgtccagcgc
     4441 ttcgcggtac gctttgatgg tctggttgaa ctccgccaga atccagcgat ccgccagcga
     4501 cagcgtcatc tcgccgccgt tgaagccgca atcctgacct tctgtgttca tcagcacaaa
     4561 gcggctggcg ttccacagct tgttacagaa gttacggtaa ccttccaggc gcttcatatc
     4621 ccagttgatg tcacgaccgg tagacgccag tgccgccagg gtgaagcgca gggcatcggt
     4681 gccgtgcggc tcaatgccgt tcgggaactg tttctcggtg cgcttacgga ttttgtccgc
     4741 cagctgcggc tgcatcatat tgccggtacg tttttccagc agttctggca gcgaaatacc
     4801 gtcaaccata tccagcgggt cgataacgtt acccttggat ttggacatct tctggccttc
     4861 gtcgtcacga atcagaccgg tcatgtaaac agtgtggaac ggcacctgcg gtttgccatt
     4921 ttcatctttg atgaagtgca tggtcatcat gatcatgcgg gcaatccaga agaagatgat
     4981 gtcgaaacca gataccatca cgctggttgg gtggaactga cgcagggcgt cggtgttttc
     5041 cggccagcca agggtagaga aggtccacag cgcagaagag aaccaggtat cgagaacgtc
     5101 ttcgtcctga cgcaggacaa catcagcacc gaggttattt tctttacgca cttcgtcttc
     5161 gttgcggcca acataaacgt tacccgcttc gtcataccat gccgggatac ggtgacccca
     5221 ccacaactga cgagagatac accagtcctg aatatcgcgc atccaggaga agtacatgtt
     5281 ttcgtactgc ttcggtacga actgaatgtc gccgttctca accgcttcaa ctgccggttt
     5341 cgccaggaca tcggcacgca cgtaccactg gtcggtcagc attggttcga tgaccacgcc
     5401 gccacggtcg ccgtaaggaa cggtcaggtc atgtggttta atttcttcca gcaggccgag
     5461 tgcgtcaacc gcagcaacga ccgctttacg tgcagcaaaa cgctccagtt tctggaattc
     5521 tgccgggatc tcgctggaat aaacgtcaga ttcgttacct ttggtatcga acacctgggc
     5581 actttcacgg atatcgccgt caaaggtcag gatgttgatc atcggcaggg cgtgacgttt
     5641 accgacttca tagtcgttaa agtcgtgcgc cggggtgatc ttcacgcagc cggtgccttt
     5701 ttccatgtcg gcgtgttcgt cgccaacgat cggaatacga cggttaacca gcggcagaat
     5761 gacatatttg ccaatcagat ctttgtaacg cggatcttcc gggttaacgg ctacgccagt
     5821 atcgcccagc agggtttccg gacgggtagt cgcaaccacc agataatctt taccgtctgc
     5881 ggttttcgcg ccgtcagcca gcggatagcg gatgtgccac atcgaacctt tcgattcgcg
     5941 gttttccact tccaggtcag agatagcggt gcgcagtttc gggtcccagt ttaccaggcg
     6001 tttgccacgg taaatcaggt cttctttata cagacgaacg aaaacttctt tcaccgcatt
     6061 ggacaggcct tcgtccatgg tgaagcgttc acgctcccag tccacggagt tgccgagacg
     6121 gcgcatctga cgggtaatgg tgccgccaga ttccgctttc cattcccaga ttttgtcgat
     6181 gaaagcttcg cggccgtagt cgtgacgggt tttaccttct tctgcggcaa tcttgcgctc
     6241 aacgaccatc tgggtagcga tcccggcgtg gtcagtaccg acctgccaca gggtgttttt
     6301 gccctgcatg cgctgatagc ggatcatggt atccatgatg gtttgctgga aggcgtgacc
     6361 catatgcaaa ctgccggtga cgttcggcgg cgggatcatg atgcagaaac tttcctggct
     6421 ttcatcgcca ttaggcttaa agtagccctg cttttcccag tgctcgtaaa gcggctgttc
     6481 gatatcttgt gggttatatg tcttttccat tatttccagg ttgccgtatt caggttgaaa
     6541 ccagccacgc ggtaggcttt atagcgttcg cgcgccagtt gtttcagaga atcttcataa
     6601 ggaacgaagt ctaccacttc tgtgaaagcg gtggcaaaat ctgcaaagct tgttcgcaga
     6661 ctaatcaata tatcgcgcgg gctgctgctg cgtttttgcg gccaggcgat ctccaccggc
     6721 gcaccgccgc gcggtccttc tcccgctaaa ttatgcggaa caaagctttc tgccggacgc
     6781 gcccacaggg cttcatccag ccggtaagcc tgcttttcat cttcacaggc gatgagcacg
     6841 cgcttaccac tgcgccaacg ttctgcggca atttcacaca ccagctgctc aacggcgctt
     6901 aagccatcga cggtggtgtc attgtccaga aggtagaacg tcgcgttttt cataaatggg
     6961 gcttcttgtg gtggatttaa atattgctgc attcctcacc ctaaaagatg agcaatgaat
     7021 ttctattgcg ctcgatcggt tccctctccc ttagggagag ggttagggtg agggtaaaac
     7081 gcaaacttac tcttcgccgt taaacccggc gcggtttaac aggaactgtg ccagcaacgc
     7141 taccggacga ccggttgcgc ctttcgcttt accagaacgc caggcggtac cggcgatatc
     7201 caggtgcgcc cagttgtact tacgggtaaa gcgtgacagg aagcaacctg cggtaatcgc
     7261 cccaccagga cgaccgccaa tgttcgccat atcggcaaaa ttggactcca gttgttcctg
     7321 atactcgtca cccagcggta agcgccatgc gcggtcaccg gattgttcag acgcggcaat
     7381 cagttcatgg gccagcggat tgtggttcgc catcagacca gtaatgtgat gacccagcgc
     7441 gatcacgcag gcaccggtca gcgtcgccac gtcaatcacc gcttccggct caaaacgctc
     7501 aacgtaagtt aacacgtcgc acagtaccag gcggccttca gcgtcggtgt tgagcacttc
     7561 aacggtttga ccagacatgg tggttaacac atcgcccgga cgataggcgc gaccgcctgg
     7621 catgttttca caacctgcca acacgccgat aacgttaatc ggcagttgta gctccgcgac
     7681 catccgcatc acgccgtaaa ccgctgccgc accgcacata tcgtacttca tctcatccat
     7741 gccttctgaa ggcttgatcg agataccgcc ggagtcgaag gttaaacctt tacccaccag
     7801 cacgattggg cgtgcatctt ccgacgcgtt gcctttgtac tcaatcaccg acatcagcga
     7861 ttcgttttgc gaaccctgac cgaccgccag ataggaatgc atccccagct ctttcatctg
     7921 ctgttcgccg ataacgcggg tgataacatt cttgctgtag ctgtcagcca gctggcgcgc
     7981 ttgtgaagcg aggtaagcgg cgttacagat attcggcggc atattgccaa gatcctttgc
     8041 tgctttaatc ccggcggcaa tcgccagacc gtgctggatc gcgcgctcac cgctggtcag
     8101 ttcacggcgg gtcggcacgt tgaacaccat tttacgcagc ggacgacgcg gttcgctctt
     8161 gttcgttttc agctgatcga aactgtagag cgtctctttt gccgtttcga cagcctgacg
     8221 cactttccag tagttgttac ggcctttaac gtgcagttca gtcagaaagc agaccgcttc
     8281 cattgagcca gtatcattca gcgtattaat ggttttctga ataacctgct tgtactgacg
     8341 ctcatccagc tcacgttctt tgccgcaacc aataaggaga attcgctcgg aaagtacatt
     8401 cggaacatgg tgcagcaaca atgtctgccc cggttttcct tccagttcgc cccgacgtag
     8461 cagggcgctg atgtacccat cgctgatttt atcgagctgt tctgcaatcg gagaaaggcg
     8521 acgtggttcg aagacgccca cgacgatgca ggcactccgc tgtttctccg ggctaccgct
     8581 ttttacacta aactccatgc actacgctcc tgaatcttaa agacaacggc ggtggctaca
     8641 gatagaattg caagctttcg taactcatgt ccgctgttgc gatgacttcg tgttaatctt
     8701 aacgttatta cggcattggc acgtcagaac aaaatctgag aggtgaatcc gttgagtata
     8761 attatcttag cgacgatttc gacgactcaa gagaataaat gacgtttaag ccatgaaaca
     8821 agctaaaatc ctgcaaaaga cgagttttta cgggcgtatt taaagtgata atcataagat
     8881 atctggtgcg ggagacgctc aaaagccagc tggcgatact cttcatcttg cttttgatct
     8941 tcttctgtca aaagttagtg aggatcctcg gcgcagcggt tgacggcgat attccggcga
     9001 atctggtgct ctcccttctc gggttgggcg tgccggaaat ggcgcagctt atcctgccat
     9061 taagcctgtt cctcgggctg ctgatgacgc tgggcaaact gtataccgaa agtgaaatta
     9121 cggtaatgca tgcctgcggc ctgagcaaag cggttctggt gaaagcggca atgatccttg
     9181 cggtattcac ggcaatcgtt gcggcggtta acgtgatgtg ggcgggaccg tggtcatcgc
     9241 gtcatcagga tgaagtgtta gcagaagcga aagcgaaccc tggcatggcg gcgctggcgc
     9301 aagggcaatt ccagcaagcg actaatggca gttcggtgct gttcatcgaa agcgttgacg
     9361 gcagcgattt caaagatgtg ttcctcgcgc aaattcgacc aaaaggtaat gcacgtcctt
     9421 ctgtggtggt ggccgattcc ggacatttaa cccagctgcg cgacggctcc caggtcgtca
     9481 ctctcaacca gggaacgcgc ttcgaaggca ctgcactgtt acgtgatttc cgcattacgg
     9541 acttccagga ttatcaggcg atcattggtc accaggcggt ggcgctcgac ccgaacgata
     9601 ccgaccagat ggacatgcgt acattgtgga acactgacac cgatcgtgcc cgcgcagaac
     9661 tgaactggcg tatcacgttg gtattcaccg tgtttatgat ggcacttatg gtcgtaccgt
     9721 tgagcgtggt taacccacgt cagggacgcg tactgtcgat gctgccagcc atgctgctgt
     9781 atctactttt cttcctgatc cagacctccc tgaaatcgaa cggcggtaaa ggtaagctgg
     9841 acccgacgct gtggatgtgg accgttaacc tgatttatct ggctttggcg attgttctca
     9901 acctttggga cactgtgccg gtccgccgcc tgcgcgccag tttttcgcgt aaaggagcgg
     9961 tgtgatgcaa ccttttggcg tacttgaccg ctatatcggt aaaactattt tcaccaccat
    10021 catgatgacg ctgttcatgc tggtgtcgct gtccggcatt atcaagtttg tcgatcagct
    10081 gaaaaaagcc gggcagggga gttacgacgc gttaggcgca ggaatgtata ccttgctgag
    10141 cgtgccgaaa gatgtgcaaa tcttcttccc gatggcggct ctgcttgggg cgttgcttgg
    10201 tcttgggatg ctggcgcagc gcagcgaact ggtggtgatg caggcttctg gttttacccg
    10261 tatgcaggtg gcgctgtcgg tgatgaaaac cgccattccg ctggtcttgc tgacgatggc
    10321 gattggtgaa tgggtcgcgc cgcagggcga gcagatggcg cgtaactacc gtgcgcaggc
    10381 gatgtacggc ggctcgttgc tctctaccca gcaaggctta tgggcgaaag atggcaacaa
    10441 cttcgtctac attgagcggg ttaaaggtga cgaagagtta ggtggcatca gcatttatgc
    10501 ctttaacgag aatcgtcgtc tacaatccgt acgctatgcc gctactgcga agtttgaccc
    10561 ggaacataaa gtctggcgtc tgtcgcaggt tgatgaatct gatttgacca atccgaaaca
    10621 gattaccggt tcgcagacgg tgagcggcac ctggaaaacc aacctcacgc cagacaaact
    10681 gggcgtggtg gcgctggacc cggatgcact ctcaatcagc ggtttgcaca actacgtgaa
    10741 gtatctgaag tcgagcggtc aggatgccgg acgttatcag ctcaatatgt ggagcaaaat
    10801 cttccagccg ctatccgtgg cggtgatgat gctgatggcg ctgtcgttca tctttggccc
    10861 actgcgtagc gtaccgatgg gcgtgcgtgt ggtcaccggt atcagcttcg gttttgtctt
    10921 ctacgtactg gaccagatct tcggcccgct gacgttggtt tatggcatcc cgccgatcat
    10981 cggcgcactg ttgccaagcg ccagcttctt cttaatcagc ctgtggctgt taatgagaaa
    11041 atcgtaacca agcaagggag cgaaagctcc ctttcacatt gttgataaag tcaaatacga
    11101 gttcgatcgg tcccctcgcc cctttgggga gagggttagg gtgaggggca cagaccgcac
    11161 ctgacttacc ttcttcttcc ccccagcaaa ctccccaaca tcccgcgtac aatctgattc
    11221 gtcacctggc gggcggcgct tttcgccatt gtttgcacca caccatcttt cttcccgccg
    11281 cgtggtccgg tagtgccaaa caaaatatcc tttaatccac caagaatgcc gtcatccacc
    11341 gccacctctt tccctttcgc ggggggatta ttttgctgct cggcgcttgc ctgtacgcct
    11401 ttttgcaaca tctcataggc ggattcccgg tccacctcat cctcgtactt gccatacacc
    11461 ggagagtgat taatcaagcc attacgctca tcttccgtca ccggtcccat ccgcgaacaa
    11521 ggcgcaatca ccatcgcccg ttccaccact gaaggacttc ctttcacatc aagaaacgag
    11581 attaacgcct cgccggtccc cagttcctgg attgcctttt cggtatcaaa tgccggattg
    11641 gcccgcatgg tttgcgccgc agcttttacc gctttctgat ctttgggcgt aaaagcacgc
    11701 aaagcgtgtt gaacgcgatt accgagctgc ccgagcacat tatccggaat atcagacggg
    11761 ttttgcgaaa cgaaccagac gcctacgcct tttgagcgaa taagccgtat cacctgctca
    11821 atcttatcca gcagtacctg cggtgcatcg ttaaacagca gatgtgcctc gtcgaagaaa
    11881 aacaccagtt ttggtttctc cagatcgcct gcttccggca attgttcata caactctgaa
    11941 agcatccaca gcaggctggc ggcgtacagt ttcggcatct gataaagctt ctcggcgctg
    12001 aggatattga taacgccttt accgttggta tcggtgcgca tccagtcttt gatatccagc
    12061 atcggctcgc caaagaagtg tgctgcgcct tgctgttcca gcgataacag cccgcgctgg
    12121 atggcaccaa ccgatgcgct actaatatta ccgtactgat tctggaaaga tttggcgtta
    12181 ttgccgatgt actgggtaat cgcccgcaaa tctttaaagt cgagcagtaa cagcccctga
    12241 tcgtcagcaa tgcggaagat gatattcagc acgccagatt gcacatcgtt gagattcaac
    12301 agccgcgcca gcaacagcgg ccccaggtct gaaaccgtcg cccgcaccgg atggcctttc
    12361 tcgccaaaga tatcccacac caccaccgga ttggcatgcg gttgccagtc attgacgccg
    12421 atatttttaa gccttgcgag cagtttttcc gacgccgttc ctgcctgcgc gataccggtc
    12481 agatcgcctt tcacatcagc cataaacacc ggcacgccga tttccgacaa tgactccgcc
    12541 agtttttgca gcgtaacggt tttacccgtc cccgttgcgc cagtaatcag cccgtgacgg
    12601 ttagccattc ccggcagtaa aaacagttct gtgtccggcg tgcgggcaat taacaggggt
    12661 tcgctcatga gatttcctcc agtttcctgc ctggagtata ggcaacgtcc ggcaaagaga
    12721 atacgtgtaa tgaagagaaa ataacctaaa gcgtgttgcc ttgataaatc tgatacccga
    12781 ggtcaacagt gttgtggttg tgatcgttat ttttaatttt gctcagcaac atttgggcgg
    12841 ccatccgacc aatatcaaaa cggggagtaa ttacgctggc gaggcttggg atcatttgcc
    12901 tgccaatctc cagcccatga aaacccgcaa tggaaatttg ttctggcacg gcgaggtttc
    12961 gctcgcggca cagcagaagc gcacccatcg caatatcgtc attggtgcaa aatacaccgt
    13021 ctaaatccgg gtttgcgcta agggcatcac gcattaactg cattcctaaa tgaatggatg
    13081 agatggcgcg tggattcatg cgtaatgggg aaagattatg cagcatcatc gcatcgcaat
    13141 acccctgata acgctgttca tcgcgggtgt catctttgga accgagatac agaattttgt
    13201 gcctgactcg cttctccagc atggtacaca ccatgtcaaa agcggcctgc cgattatcaa
    13261 aaccgacctc catatccagc cgttctccct gtacatccat caattccacg accggaatgg
    13321 tggctgaacg cagaaatttc accgtcctga tggtgtgata tttttccgaa agtataatcc
    13381 cgtcaatgtt ataagagagc aggttaatca ccgactcttc ttcggaatcg cgatcgtaat
    13441 tgtaattagc aataagggtc tgataattat gctcagaggt gacggattct attcccgcga
    13501 gaatgtcagc gaagagttga ttttgaaatg acggaatcaa gataccaagg gtataacttt
    13561 gtgcattcaa caacatgccg ggagcgcgat tagggatata attgatctct tccataattt
    13621 tggcgatgcg ctcgcctgtt tcctttgcca cctttttcgg cgagcggata taacgactca
    13681 cggtcatttt tgttacgcca gccagcgtag cgatatcctg taaagaaatt ctgtgattgc
    13741 gcataacttc tcccaacgtc gggggaatct tcaatcttcc cccgaaacta catcacaata
    13801 ttttattctt tcagtgcaat acggcgttaa tggctaacac gcccagcaaa cccataatag
    13861 aaataagggt ttccatcacc gtccaggtac gcaacgtttc accaaccgtc agattaaaat
    13921 accctttaaa tagccagaag ccagggtcgt ttacgtgtga cgcgatcacg ctgcccgcac
    13981 cggttgccag taccattaaa gcgggatcgg catgggtaac gttgataatc ggcaacacca
    14041 cacccgcggt ggtaatggcc gcgacggtgg cagagcccag agcgatacgc aacagcgccg
    14101 caacagtcca gcacatcaat aacggcgaaa gcgtagttcc ggtcattaag tgtgaaatat
    14161 agtgcccgac accgctatct actaatacct gcttaaacgc gccgccgcca gcgataataa
    14221 acacaatcat cgcgatagcg cctatagaat ccccaatgat atccatgatt tgctcgatgg
    14281 tgcgtccatt acgtcggccc agtgtgaaaa tcgcaataac aatggcaata aacagcgcaa
    14341 cggcagggtt accgacaaat tcaaagaaga ggcgcacggt gttagttttc ggtaacgtaa
    14401 tttcacaaac ggcggcgata gccatcagga tgaccgggat cacggcagcg aaaatactgt
    14461 tccagaagga gggcatctcc tcttcgctaa acagatgagg attaaataag ccttccggtg
    14521 gtgctttctc aaagcgagtt agcagtttag aaaacagcgg tcctgcgaca ataactgtcg
    14581 gaatggtaat gataaatcca tacagtaaag tcgttccgag attagcctca aagatagtcg
    14641 cgatggcagt aggccctgga tgtggcggca gaaaacagtg ggttacagag agcgctgcta
    14701 ccattggtac gccaacatac aacaggggta atcctgatga tgctacgatg gtaaatacca
    14761 acggcaacag caggacaaaa cccacttcaa aaaacatggc gaggcccaca accagaccgg
    14821 tgatcactag cgcccattgc acgcgttttt taccaaaagt agcaatcagc gtagtggcga
    14881 tacgttgtgc cgcacccgta tcagaaatca gcttgcctaa catggccccg aaaccgagaa
    14941 tcattgccag cccgccgagc gtgctgccga taccattttg tatagagtgc aggacggcct
    15001 gtgcatccat cccttcggca aatccgacga cggcagctac cagaacgagg gcaataaagc
    15061 cgttaacttt aaagccgatc atcaggataa gaagcagcgc gacgcctgcc gcaataatga
    15121 ttaatggcat agtctttatc tcttattagt gccagaaaag cagcgctttc gcgctgcacg
    15181 tagggtagta ggtgttaaac agccactaac atgccgccat caacaaacaa caggtggccg
    15241 tttacgaaat cagaggcttt tgaagaaagg aacaccgcag caccaatcag ttcctgcgga
    15301 tctccccagc gtgcggcggg ggtccgtttg cacaaccagg cggtgaaggc ttcgtcctca
    15361 accagtgctt tagtcatttc tgttttgaaa tagcccggcg caataccgtt gacctgaata
    15421 ttgtggcgcg ccagctcgac gcacatgccg cgggtgagca tttttaccgc ccctttcgat
    15481 gcggcataag gggtgatggt gtcacgtccc agttcgcttt gcatcgagca aatattaata
    15541 actttacctg ccttgcgttc aaccatgtga cgagttaccg cttgcgatac caggaacacg
    15601 gcggtctggt ttactgcgat cacatcattc cactcttgtt cagggaactc agtaaaagga
    15661 tgacggcgct ggataccggc gttattcacc agcacatcaa tggggccgat gtccttttcg
    15721 atatgttcaa cggcggcatc aatttcatgt ttatgagtaa cattaaaagg tgcggcaacg
    15781 gcctgaatac cctcctggtg gagtttttct acagcaagtt ctgcgcgttc ggcagtaata
    15841 tcattaataa ttatttgtgc gccatattta cccaggccgg ttgccagtaa aaagccaatg
    15901 ccctgtgctg aaccggtaat caagatattt tttcctgcca gtgaaaatag atcgttcatt
    15961 gtattattcc tttatttatc tcttagaaaa caagctggac ttttgctgcc tgggttttat
    16021 caccggcgaa acgtagcgcc tcttccaggt cagtgaaggg atattcagca ctcagtaaag
    16081 gcagtggatt gataacgcca ttcgccagcc atgacactgc ggtattaaat tcgctggtaa
    16141 aacggaaaga gcctctgagt gaaatctcct taccaatcaa cgtcatcatt gggaattctg
    16201 ccatcgcgcc tcccatacct acctgcacca ttacgccgcg tgcacgagtg acctccagac
    16261 aggtattcac tgatgaagga tgaccggaca cttcaaagct gacatcgaaa tagccttttt
    16321 ccgctttcca gtgatccatg tcgtcgtttt gtgggtttac gagcacatcc gcccccatct
    16381 ctttgcccag cgaaagggaa cggggactca catcagcaca gacaatttcc gcggccccca
    16441 gtgttttcac tgcactgaca atcaggcagc caatgggtcc aacaccggaa ataaatactc
    16501 gcttgccctg taactcgccg gcctgatgtg cggcatgaat cgcgacggct aaaggttcgg
    16561 caaaagccat aaccttttcg tcagctttgg ccggataagg gacacattgc gacgtttcga
    16621 ccattttata acgggtaaaa ccaccatcaa catgagggaa atacatggca ctgccaaaaa
    16681 aacgcatctc tgtacactga ttctcgttat gttcaatgca gtatttgcag tgaccgcacg
    16741 gtttagacgg attaatggct accgtttgcc cttcatgtaa ttctgatgag tcgctatgaa
    16801 taactttacc gataacttca tgacctaaca ccatcggtgc ctttatcatg aaattaccta
    16861 cttttccttc ctgataataa tgtaaatcgg aaccgcaaat tccacctcgg gttatttgta
    16921 ctaatgttcc attattattc caatctatcg tctgctcggt aacggcaaca gttttcttgc
    16981 ccgcaacaac gcaggactgt gttttcactt gcataaattc ctcactggtc aggtagacac
    17041 ctcggaagca tttaagcggt tttaattgtc attatttgtg atgaagatca cgtcagaaaa
    17101 ttgttacatt actatgttac gcataacgtg atgtgccttg taattcttat cagcagaaat
    17161 aaaaaaacgt gaaattatta tgccaccagg cgtagtatcg cagcaggtaa gatgattcag
    17221 gagattttaa atggcgggtg aaagctttat tttgatgggc gtttcaggga gtggtaaaac
    17281 attaattggt agcaaggttg ccgcgttatt atctgctaaa tttattgatg gtgacgatct
    17341 tcatccagcc aaaaatatag ataaaatgtc gcagggtatt ccattatctg atgaagatcg
    17401 acttccctgg ctggaacgct taaatgatgc ttcatacagt ctttataaaa agaatgaaac
    17461 aggatttatt gtctgttcat cattaaaaaa acattatcgt gatattttac gcaagggtag
    17521 cccccatgtt catttcctct ggttagatgg cgactatgaa actatcctcg cgcgaatgca
    17581 gcgtcgggct gggcatttta tgccggtagc gttactaaaa agtcagtttg aggcgctgga
    17641 gcgtccacaa gcagatgaac aggatattgt gcgcattgat atcaaccatg atattgccaa
    17701 tgtcaccgaa caatgtcggc aggctgtgct ggcgatacga caaaacagaa tatgtgcgaa
    17761 agagggcagc gcctcagatc agcgctgcga atgattttca aaaatcggct ttcaacacca
    17821 cgcggtaacg cgccttaccg tcgcgcacat gctggatggc gtcgttaatt ttcgacatcg
    17881 ggaacagttc ggttgtcggc gcaaccttgc tgcgggcggc aaagcgcatc agcttacgca
    17941 gttcataagg cgtgccggta gcagagccag agacgctgcg atcgcccgca attaacgtaa
    18001 aggccggaac agacagcggc gtgagaaccg caccgacagt gtggaaatta ccgccgtacg
    18061 tcagcgcctc aaaataaggc tgccagtcga ggctgacgtt cacggtattg ataatgagat
    18121 caaactgccc cgccagtgct ttcagtgcct gcggatcgcg gctattcacc actttatcgg
    18181 cacccatcgc cagcaattcc tgctctttcg ccggattaga actaaaggcc gtcacctcac
    18241 atcccattgc gtgcagaagt tttatagcga tatgccccag cccgccaata ccaattaccc
    18301 caacgcggct ggtagcagtg atatggtgca tcaacagtgg tttaaagacc gtgataccgc
    18361 cgcacaacag cggcccggca gatttaatgt cgatattttc cggcagtgga ataacccatt
    18421 gccagtctac acgcaacttc tcggcaaaac ctccgcgatt cataattgtt ggcaccgcac
    18481 cttgctcaca gttgatctga tttccgctaa tacaggcgtc gcagtgacca cagctacgcg
    18541 ctgtccagcc aatcccgaca cgctgaccga cctgcaaacc tttatcctgc gcggcactcc
    18601 cgagcgccac cacgcgacca atcacctcat gcccggcaac cagcggatat tgtgaaaatc
    18661 cccattcgtt atcgatcatc gacagatcgg aatggcagat cccgcagtaa tccacctgca
    18721 cttcaacatc ttgtggcttc agctcaccgg gatcgtactc ataaacttcc agttcgccgc
    18781 ccgcttcttt tgcggcatag ctttttatca ttgacatttt ttggtccttc tctggtgtgg
    18841 tctgggaagt gtagagcatg gcagggaggg gtgcatggag tgtgacaaag gttacacatc
    18901 gctgtacgca atgctgaaaa tttcagcact tagcgagatg cgagcaagct ggcgcttgca
    18961 tggtggcgtg cgacaggtat aatccacaac gttttccgca tacctcttca gtgccgaagt
    19021 ggcgaaatcg gtagacgcag ttgattcaaa atcaaccgta gaaatacgtg ccggttcgag
    19081 tccggccttc ggcaccatta acgctaatca ataacgattt ttccgcaaat caagtagtta
    19141 ggattttacc ccgactaact ttgtatgtga aaaatactaa tctcttatca agtacaaaag
    19201 ccactgtgtt tgttaatggc tttggtatgt tatcacacag tggcgagtag ttcacaggct
    19261 caacctcgta acaagttcct ttgaagctgg aagacgtgcc accgtctcta aatcgtatac
    19321 caaaactctt agcaagatgt tccacacctg gcgcggtgtc caatgatgct acacgtcgtg
    19381 agacgttgca gaggataaac aggctaaatg atgggaagag ttttaagtgc aatagtggat
    19441 taaagcccac ctcttcaaaa cctaacttga tcgggaaaga atcaagctct agcacggttt
    19501 cgtccaccgg agtacaacgg gtgcctgata agagaagaac tgctaagact tcatagcttt
    19561 tgtgaaaaag ccctttttag tggatacgcc tacggcgctt attttttggg atagcccatc
    19621 aaaggcgctt tatggctcct attgtctaaa aataatcctc ctggtatttt aagcatctaa
    19681 gatggtttga atgatggggg atgtatggcg atatcagcaa agaaagccgc ctacaaagaa
    19741 aagaaagcaa aagccgcaga actagccaaa ctacgccgcc agcaaaaagc agcattaacc
    19801 agaatgataa agaaggctag agaagacggc acatttgaca gcttgccaga cgatatcaaa
    19861 gcaaagtgcc ttaaacacga agcaaagcgg gaaaaggcag catacttcta taaccagtgg
    19921 aaagagaagc aagcatacgc cgcacgtcgt gaagaggtgg cgggtattga tgaattatcc
    19981 ctgcaacact tcaaatcaat ttaaatactc cctgattctc taaaacgcaa caggacgcat
    20041 tctaacgctg ttttcattgc ttggtatagg gatgtatagg tgatgtgtgt ttaatgcgtc
    20101 tgtgagcttg ttttgtgcct tattttgcat tttcataatg cagaagagaa cacggaaagc
    20161 cttaaaatta attattgggg
//
LOCUS       DAZFQD010000096        19929 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.096, whole genome shotgun sequence.
ACCESSION   DAZFQD010000096 DAZFQD010000000
VERSION     DAZFQD010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19929
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.096"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..504)
                     /locus_tag="ACHKTW_003416"
     CDS             complement(<1..504)
                     /locus_tag="ACHKTW_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001682040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase N-terminal domain-containing
                     protein"
                     /protein_id="HFO6464372.1"
                     /translation="MTECYIPRSVDAEQAVLGGLMLDNDRWDEVVLMLRGEDFSLAVH
                     RVIFRSMSELAVSGQPFDLITLSEHIEKMHNIEALGGFAYLAELSKNTPSAANIMAYV
                     RIVAEKSMLRQLQETGNSLVADVASPDATPRAVLESAERRLFSLSQTGMLHERSEVSL
                     SSAMELAL"
     gene            complement(855..1757)
                     /locus_tag="ACHKTW_003417"
     CDS             complement(855..1757)
                     /locus_tag="ACHKTW_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6464373.1"
                     /translation="MSSSVKYSPVPAVLTRPELLAELNLLMTRIASGCRVGVTASCRP
                     VIAVHGGYVSYSSPRAGRIFQDGSRPEIVMAVSNRERMCEWFSQVWLPLHLAALEGDE
                     SAFFSAEVNMVRRELQRVPGNSVNCMCWASLAALAFDAEERVELQKGNNENIATYDES
                     IATYAFRAACTVSRCLSRNVSGGIPRSYPEWKAILFPRDGADIFPDGWLENMTRRAER
                     VFTSAKRQCGMLSASQAALFSQQTRQCASEQCSGRIQAGSHHSGSHPDVSAYEVRCAQ
                     EMSLSPVHSTRQCSVQSTDSAETE"
     gene            2081..2425
                     /locus_tag="ACHKTW_003418"
     CDS             2081..2425
                     /locus_tag="ACHKTW_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001682037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6464374.1"
                     /translation="MSTDDVARCKTFFDQGTEIFPGADQKLAYRLCWPGHWLINSRRW
                     FFLDDNSISIQQAVKFALIHQHHWLPATVEQMPPAEMSLALTDYWADYWTQPTEPEKA
                     LVHMTDWQYLNH"
     gene            complement(2419..3297)
                     /locus_tag="ACHKTW_003419"
     CDS             complement(2419..3297)
                     /locus_tag="ACHKTW_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFO6464375.1"
                     /translation="MKILPVVSTKGGEGKSTQSANLAGFLADAGIRTLLIDGDHAQPT
                     ASNIFPLTYEAPAGLFELLMRTADLTHPDNIISRTAIDGLDLIVSNDPHEQLKTAMLH
                     APDGRLRLRNVLQHPLFLNNYDVIVVDSQGARSVMLEMIVLSATESVVGMVNPVLPDV
                     REFIRGTVNVMENLLPYRELGIPLPKVRTLVNCMDYTALARKTLDELAGIIQTGRYSR
                     QLPEGAVSLLSTQIYDLNIYKQGHAAGQPVHRLEKESTRRSDSALVTMHSLACELFPE
                     WKPAFDGLLENGGTRQ"
     gene            complement(3546..4082)
                     /gene="yghD"
                     /locus_tag="ACHKTW_003420"
     CDS             complement(3546..4082)
                     /gene="yghD"
                     /locus_tag="ACHKTW_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspM family type II secretion system protein
                     YghD"
                     /protein_id="HFO6464376.1"
                     /translation="MLRDKFIHYFQQWRERQLSRGEHWLAQHLAGRSPREKGMLLAAV
                     VFLFSVGYYVLIWQPLSERIEQQETMLQQLVAMNTRLRSAAPDIMAARKSATTTPAQV
                     SRVISDSASAHSVVIKRIAERGENIQVWIDPVVFNDLLNWLNALDEKYALRVTQIDAS
                     AAEKTGMVNVQRLEFGRG"
     gene            complement(4084..5262)
                     /gene="gspL"
                     /locus_tag="ACHKTW_003421"
     CDS             complement(4084..5262)
                     /gene="gspL"
                     /locus_tag="ACHKTW_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000097227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HFO6464377.1"
                     /translation="MSSILEIFFPLCASDTVRWQRRTPDVEHGIWPDVADEQLQQWLQ
                     TDAIRLYIPGEWISVWQVELPDVARKQIPTILPALLEEELNQDIDELHFAPLKIDQQL
                     ATVAVIHQQHMRNIAQWLQENGITRATVAPDWMSIPCGFMAGDAQRVICRIDECRGWS
                     AGLALAPVMFRAQLNEQDLPLSLTVVGIAPEKLSAWAGADAERLTVTALPAVTTYGEP
                     EGNLLTGPWQPRVSYRKQWARWRVMILPILLILVALAVERGVTLWSVSEQVAQSRTQA
                     EEQFLTLFPEQKRIVNLRSQVTMALKKYRPQADDTRLLAELSAIASTLKSASLSDIEM
                     RGFTFDQKRQTLHLQLRAANFASFDKLRSALATDYVVQQDALQKEGDAVSGGVTLRRK
                     "
     gene            complement(5259..6236)
                     /gene="gspK"
                     /locus_tag="ACHKTW_003422"
     CDS             complement(5259..6236)
                     /gene="gspK"
                     /locus_tag="ACHKTW_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HFO6464378.1"
                     /translation="MITSPPKRGMALVVVLVLLAVMMLVTITLSGRMQQQLGRTRSQQ
                     EYQQALWYSASAESLALSALSLSLKNEKRVHLTQPWASGPRFFPLPQGQIAVTLRDAQ
                     ACFNLNALAQPTTASRPLAVQQLIALISRLDVPAYRAELIAESLWEFIDEDRSVQTRL
                     GREDSEYLARSVPFYAANQPLADISEMRVVQGMDDGLYQKLKPLVCALPMTRQQININ
                     TLDVTQSVLLEALFDPWLSPVQARALLQQRPAKGWEDVDQFLAQPLLADVDERTKKQL
                     KTVLSVDSNYFWLRSDITVNEIELTMNSLIVRMGPQHFSVLWHQTGESE"
     gene            complement(6233..6838)
                     /gene="gspJ"
                     /locus_tag="ACHKTW_003423"
     CDS             complement(6233..6838)
                     /gene="gspJ"
                     /locus_tag="ACHKTW_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspJ"
                     /protein_id="HFO6464379.1"
                     /translation="MRRTRAGFTLLEMLVAIAIFASLALMAQQVTNGVTRVNNAVAGH
                     DQKLNLMQQTMSFLTHDLTQMMPRPVRGEQGQREPALLAGAGVLASESEGMRFVRGGV
                     VNPLMRLPRSNLLTVGYRIHDGYLERLAWPLTDAAGSVKPTMQKLIPADSLRLQFYDG
                     TRWQESWSSVQAIPVAVRMTLHSPQWGEIERIWLLRGPQLS"
     gene            complement(6835..7206)
                     /gene="gspI"
                     /locus_tag="ACHKTW_003424"
     CDS             complement(6835..7206)
                     /gene="gspI"
                     /locus_tag="ACHKTW_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001709020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
                     /protein_id="HFO6464380.1"
                     /translation="MKRGFTLLEVMLALAIFALAATAVLQIASGALSNQHVLEEKTVA
                     GWVAENQTALLYLMTREQRAVRHQGESDMAGSRWYWRTIPLNTGNALLQAVDIEVSRH
                     EDFSSVIQSRRAWFSAVGGQQ"
     gene            complement(7203..7766)
                     /gene="gspH"
                     /locus_tag="ACHKTW_003425"
     CDS             complement(7203..7766)
                     /gene="gspH"
                     /locus_tag="ACHKTW_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001394829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspH"
                     /protein_id="HFO6464381.1"
                     /translation="MPERGFTLLEIMLVIFLIGLASAGVVQTFATDSESPAKKAAQDF
                     LTRFAQFKDRAVIEGKTLGVLIDPPGYQFMQRRQGQWSPVSSTRLSAQVTVPKQVQML
                     LQPGSDIWQKEYALELQRRRLTLHDIELELQKEAKKKTPQIRFSPFEPATPFTLRFYS
                     AAQNACWAVKLAHDGALSLNQCDERMP"
     gene            complement(7770..8225)
                     /gene="gspG"
                     /locus_tag="ACHKTW_003426"
     CDS             complement(7770..8225)
                     /gene="gspG"
                     /locus_tag="ACHKTW_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003912123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HFO6464382.1"
                     /translation="MNSLSRTQKPRAGFTLLEVMVVIVILGVLASLVVPNLLGNKEKA
                     DRQKAISDIVALENALDMYRLDNGRYPTTEQGLEALIQQPANMADSRNYRTGGYIKRL
                     PKDPWGNDYQYLSPGEKGLFDVYTLGADGQENGEGAGADIGNWNLQEFQ"
     gene            complement(8242..9465)
                     /gene="gspF"
                     /locus_tag="ACHKTW_003427"
     CDS             complement(8242..9465)
                     /gene="gspF"
                     /locus_tag="ACHKTW_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HFO6464383.1"
                     /translation="MALFYYQALERNGRKTKGMIEADSARHARQLLRGKELIPVHIEA
                     RMNTSSGGMLQRRRHAHRRVAAADLALFTRQLATLVQAAMPLETCLQAISEQSEKLHV
                     KSLGMALRSRIQEGYTLSDSLREHPRVFDSLFCSMVAAGEKSGHLDVVLNRLADYTEQ
                     RQRLKSRLLQAMLYPLVLLVVATGVVTILLTAVVPKIIEQFDHLGHALPASTRTLIAM
                     SDALQASGVYWLAGLLALLVLGQRLLKNPTMRLRWDKTVLRLPVTGRVARGLNTARFS
                     RTLSILTASSVPLLEGIQTAAAVSANRYVEQQLLLAADRVREGSSLRAALAELRLFPP
                     MMLYMIASGEQSGELETMLEQAAVNQEREFDTQVGLALGLFEPALVVMMAGVVLFIVI
                     AILEPMLQLNNMVGM"
     gene            complement(9465..10958)
                     /gene="gspE"
                     /locus_tag="ACHKTW_003428"
     CDS             complement(9465..10958)
                     /gene="gspE"
                     /locus_tag="ACHKTW_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HFO6464384.1"
                     /translation="MVPVAQETTANTVRLPYSFSRRFSLVAWCEASLEILHVHPLSLS
                     VLQELQRGLNAPFTLRQIDEAEFEQRLNAVWQRDSSEARQLMEDLGSAEDFFTLAEEL
                     PETEDLLESDDDAPIIKLINAMLAEAIKEGASDIHIETFEKSLVIRFRVDGTLHEMLR
                     PGRKLASLLVSRIKVMARLDIAEKRVPQDGRIALLLGGRAIDVRVSTMPSAWGERVVL
                     RLLDKNQARLTLERLGLSQQLTAQLRQLLHKPHGIFLVTGPTGSGKSTTLYAGLQELN
                     NHSRNILTVEDPIEYMIEGIGQTQVNTRVGMTFARGLRAILRQDPDVVMVGEIRDTET
                     AEIAVQASLTGHLVLSTLHTNTAVGAITRLQDMGVEPFLLSSSLTGVMAQRLVRTLCP
                     DCRQPAPATDEEKRLLGITDARTVTLYHPQGCPACNHKGFRGRTAIHELIVVDATLRD
                     LIHRQAGELELERYVRQHSAGIRSNGIEKVLAGETSLDEVLRVTMEA"
     gene            complement(10958..13018)
                     /gene="gspD"
                     /locus_tag="ACHKTW_003429"
     CDS             complement(10958..13018)
                     /gene="gspD"
                     /locus_tag="ACHKTW_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020234996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HFO6464385.1"
                     /translation="MFWRDITLSVWRKKTTGLKTKKRLLPLVLAAALCSSPVWAEEAT
                     FTANFKDTDLKSFIETVGANLNKTIIMGPGVQGKVSIRTMTPLNERQYYQLFLNLLEA
                     QGYAVVPMENDVLKVVKSSAAKVEPLPLVGEGSDNYAGDEMVTKVVPVRNVSVRELAP
                     ILRQMIDSAGSGNVVNYDPSNVIMLTGRASVVERLTEVIQRVDHAGNRTEEVIPLDNA
                     SASEIARVLESLTKNSGENQPATLKSQIVADERTNSVIVSGDPATRDKMRRLIRRLDS
                     EMERSGNSQVFYLKYSKAEELVDVLKQVSGTLTAAKEEAEGTVGSGREIVSIAASKHS
                     NALIVTAPQDIMQSLQSVIEQLDIRRAQVHVEALIVEVAEGSNINFGVQWASKDAGLM
                     QFANGTQIPIGTLGAAISQAKPQKGSTVISENGATTINPDTNGDLSTLAQLLSGFSGT
                     AVGVVKGDWMALVQAVKNDSSSNVLSTPSITTLDNQEAFFMVGQDVPVLTGSTVGSNN
                     SNPFNTVERKKVGIMLKVTPQINEGNAVQMVIEQEVSKVEGQTSLDVVFGERKLKTTV
                     LANDGELIVLGGLMDDQAGESVAKVPLLGDIPLIGNLFKSTADKKEKRNLMVFIRPTI
                     LRDGMAADGVSQRKYNYMRAEQIYRDEQGLSLMPHTAQPVLPAQNQALPPEVRAFLNA
                     GRTR"
     gene            complement(13048..14007)
                     /gene="gspC"
                     /locus_tag="ACHKTW_003430"
     CDS             complement(13048..14007)
                     /gene="gspC"
                     /locus_tag="ACHKTW_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspC"
                     /protein_id="HFO6464386.1"
                     /translation="MARVVFRDARIYLIQWLTKIRHTLNQRQSLNTDKEHLRKIVRGM
                     FWLMLLIISAKVAHSLWRYFSFSAEYTAVSPSANKPPRADAKTFDKNDVQLISQQNWF
                     GKYQPVATPVKQPEPVPVAETRLNVVLRGIAFGARPGAVIEEGGKQQVYLQGETLGSH
                     NAVIEEINRDHVMLRYQGKIERLSLAEEERSTVAATNKKAVSDEAKQAVAEPAVSAPV
                     EIPTAVRQALAKDPQKIFNYIQLTPVRKEGIVGYAVKPGADRSLFDASGFKEGDIAIA
                     LNQQDFTDPRAMIALMRQLPSMDSIQLTVLRKGARHDISIALR"
     gene            complement(14025..14435)
                     /gene="gspS2"
                     /locus_tag="ACHKTW_003431"
     CDS             complement(14025..14435)
                     /gene="gspS2"
                     /locus_tag="ACHKTW_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system pilot lipoprotein
                     GspS-beta"
                     /protein_id="HFO6464387.1"
                     /translation="MSIKQMPGRVLISLLLSVTGLLSGCASHNENASLLAKKQAQNIS
                     QNLPIKSAGYTLVLAQSSGTTVKMTIISEAGTQTTQTPDAFLISYQRQMCADPTVKLM
                     LTEGINYSITINNTRTGNQYQRKLDRTTCGIVKA"
     gene            complement(14501..15310)
                     /gene="pppA"
                     /locus_tag="ACHKTW_003432"
     CDS             complement(14501..15310)
                     /gene="pppA"
                     /locus_tag="ACHKTW_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase PppA"
                     /protein_id="HFO6464388.1"
                     /translation="MLFDVFQQYPAAMPILATVGGLIIGSFLNVVIWRYPIMLRQQMA
                     EFHGEMPSAQSKISLALPRSHCPHCQQTIRIRDNIPLLSWLMLKGRCRDCQAKISKRY
                     PLVELLTALAFLLASLVWPESGWALAVMILSAWLIAASVIDLDHQWLPDVFTQGVLWT
                     GLIAAWAQQSPLTLQDAVTGVLVGFITFYSLRWIAGIVLRKEALGMGDVLLFAALGSW
                     VGPLSLPNVALIASCCGLIYAVIIKKGSTTLPFGPCLSLGGIATLYLQALF"
     gene            complement(15450..>19929)
                     /gene="sslE"
                     /locus_tag="ACHKTW_003433"
     CDS             complement(15450..>19929)
                     /gene="sslE"
                     /locus_tag="ACHKTW_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipoprotein metalloprotease SslE"
                     /protein_id="HFO6464389.1"
                     /translation="SSSDTPPVDSGTGSLPEVKPDPTPNPEPTPEPTPDPEPTPEPTP
                     DPEPTPEPEPEPVPTKTGYLTLGGSLRVTGDITCNDESSDGFTFTPGDKVTCVAGNNT
                     TIATFDTQSEAARSLRAVEKVSFSLEDAQELAGSDNKKSNALSLVTSMNSCPANTEQV
                     CLEFSSVIESKRFDSLYKQIDLAPEEFKKLVNEEVENNAATDKAPSTHTSPVVPATTP
                     GTKPDLNASFVSANAEQFYQYQPTEIILSEGRLVDSQGDGVVGVNYYTNSGRGVTGEN
                     GEFSFSWGETISFGIDTFELGSVRGNKSTIALTELGDEVRGANIDQLIHRYSKAGQNH
                     TRVVPDEVRKVFAEYPNVINEIINLSLSNGATLGEGEQVVNLPNEFIEQFKTGQAKEI
                     DTAICAKTDGCNEARWFSLTTRNVNDGQIQGVINKLWGVDTNYKSVSKFHVFHDSTNF
                     YGSTGNARGQAVVNISNAAFPILMARNDKNYWLAFGEKRAWDKNELAYITEAPSLVEP
                     ENVTRDTATFNLPFISLGQVGEGKLMVIGNPHYNSILRCPNGYSWNGGVNKDGQCTLN
                     SDPDDMKNFMENVLRYLSDDKWTPDAKASMTVGTNLDTVYFKRHGQVTGNSAAFDFHP
                     DFAGISVEHLSSYGDLDPQEMPLLILNGFEYVTQVGNDPYAIPLRADTSKPKLTQQDV
                     TDLIAYLNKGGSVLIMENVMSNLKEESASGFVRLLDAAGLSMALNKSVVNNDPQGYPN
                     RVRQQRATGIWVYERYPAVDGALPYTIDSKTGEVKWKYQVENKPDDKPKLEVASWLED
                     VDGKQETRYAFIDEADHKTEDSLKAAKEKIFAAFPGLKECTNPAYHYEVNCLEYRPGT
                     GVPVTGGMYVPQYTQLSLNADTAKAMVQAADLGTNIQRLYQHELYFRTNGRKGERLSS
                     VDLERLYQNMSVWLWNDTSYRYEEGKNDELGFKTFTEFLNCYANDAYAGGTKCSADLK
                     KSLVDNNMIYGDGSSKAGMMNPSYPLNYMEKPLTRLMLGRSWWDLNIKVDVEKYPGAV
                     SEEGQNVTETISLYSNPTKWFAGNMQSTGLWAPAQKEVTIKSNANVPVTVTVALADDL
                     TGREKHEVALNRPPRVTKTYSLDASGTVKFKVPYGGLIYIKGNSSTNESASFTFTGVV
                     KAPFYKDGAWKNDLNSPAPLGELESDAFVYTTPKKNLNASNYTGGLEQFANDLDTFAR
                     SMNDFYGRDETSGKHRMFTYKALTGHKHRFTNDVQISIGDAHSGYPVMNSSFSTNSTT
                     LPTTPLNDWLIWHEVGHNAAETPLTVPGATEVANNVLALYMQDRYLGKMNRVADDITV
                     APEYLEESNNQAWARGGAGDRLLMYAQLKEWAEKNFDIKKWYPDGTPLPEFYSEREGM
                     KGWNLFQLMHRKARGDDVGDKTFGGKNYCAESNGNAADTLMLCASWVAQTDLSEFFKK
                     WNPGANAYQLPGASEMSFEGGVSQSAYNTLASLNLPKPKQGPETINKVTEYSMPAE"
BASE COUNT         4517 a         5646 c         5035 g         4731 t
ORIGIN      
        1 cagggccagc tccattgcgg aggaaaggct cacttcactg cgctcatgca gcatgcctgt
       61 ctgggaaaga gaaaaaagtc gccgttccgc ggattcaaga actgcgcgcg gagtggcatc
      121 cggcgatgca acatcagcaa ccagagaatt accggtttcc tgaagctgcc gcagcatact
      181 cttttccgcc acaatacgga catacgccat gatattggct gctgaaggtg tattcttact
      241 gagctcagcc aggtaagcga agccgcccag tgcctcaata ttgtgcattt tctcaatatg
      301 ctcactcagc gtgatgaggt cgaacggctg accgctgact gccagctctg acatagagcg
      361 gaatatcacc cggtgcacag ccagagaaaa atcctccccc ctgagcatca gcaccacttc
      421 atcccagcga tcattatcaa gcatcaggcc gccaagcaca gcctgttcag catcaacgga
      481 gcgcggaata tagcattcag tcatggcgtc ccctccctct ctgactgccg gaaacctggc
      541 tatgtgaaaa aaggaaaaag ggtgccggac atacagttgt cgggaataaa atcccggtaa
      601 ctgtctgtac gttttcagac cggcgagttt gccgcccccg gtcagaaggt acggacagca
      661 tctttatatt cataacactt tgacttaata aggaaaaaat atgaatacaa gttatcaaca
      721 actgccgtcc ggcattggat ctgaagtgaa atacaccctg cagttctggc tgctgagtaa
      781 cggaaaggtc atgacggaaa aagacgggct ctgtaacccc ttccccccga actgtattga
      841 atcggctcat cctttcactc cgtttcagca gaatcagtgg attgaactga acactgtcgg
      901 gtcgaatgga ccggtgaaag cgacatttct tgtgcacagc gtacttcata agctgaaacg
      961 tcagggtggc tccctgaatg atggctgccg gcctgaatac ggccggagca ttgctcactg
     1021 gcacactgac gtgtttgttg agaaaaaagc gctgcctgac tggctgagag cattccacac
     1081 tgacgtttcg cactcgtaaa gacacgttca gcccgacggg tcatattttc cagccacccg
     1141 tcggggaaaa tgtcagcacc atcacgggga aataaaatcg ctttccattc agggtatgag
     1201 cgcggaatac ctccggaaac attccggctc agacagcgac tcacagtaca ggcagcacgg
     1261 aatgcatatg ttgcgatgct ctcatcatat gttgcgatgt tctcattatt gcctttctgc
     1321 agttccaccc gctcttcagc atcaaatgcc agtgcagcaa ggcttgccca gcacatgcag
     1381 ttcactgaat tccccggaac gcgctgcagc tcccggcgca ccatgttgac ctccgcactg
     1441 aaaaaagcac tctcatcgcc ttcaagggca gccagatgca gcggaagcca gacctgactg
     1501 aaccattcgc acatccgttc ccggttactg acggccatca ctatctccgg gcgggaaccg
     1561 tcctgaaaaa tacgtccggc gcgtggagaa ctgtatgaaa cataaccacc gtgcaccgca
     1621 atgaccggac gacatgacgc cgtaaccccc acacgacacc cactggcaat ccgggtcatc
     1681 agcaggttca gttcagccag caattcaggg cgggttaaca ccgccggaac cggagaatat
     1741 ttcactgagg aagacatact ttcacctcca tatctctctg cccgccaggt gcgtcacttc
     1801 cccgcagata accggaaacc tggctatgtg aaaaaaggaa aaagggcgcc ggacgaacag
     1861 ttgcagggca taaaatgccc ataactgcct gtacattttc cgaccggtga gtttggcgac
     1921 cccggtcaga aggtactggc agcattcgca tactcataac agattgtttt aaaggagaat
     1981 ttttatgaat acagaaaaac aactgccgtc cggcattgat aacaaccatg atgaggatgg
     2041 caacatggca aaatattatg aaaactggaa agcctgaggc atgagcactg acgatgtggc
     2101 acgttgtaaa accttctttg accagggaac tgaaatcttt cccggggctg accagaagct
     2161 cgcttaccga ctttgctggc ccggacactg gctgattaac agccgtcgct ggttttttct
     2221 ggacgataac agtatcagca ttcaacaagc cgttaagttt gctcttattc atcagcatca
     2281 ctggctcccg gcaacggttg agcagatgcc ccccgcagag atgtcccttg ctctgacaga
     2341 ttactgggct gactactgga cacagccgac tgagccggag aaggcgctgg ttcacatgac
     2401 tgactggcag tacctgaatc actgacgagt tcctccgttt tccagcagcc cgtcaaacgc
     2461 cggtttccac tcaggaaaga gttcacaggc caggctgtgc atcgtgacaa gcgcagaatc
     2521 actgcgacgt gtggattctt tttcgagacg atgaacgggt tgaccagccg catgtccctg
     2581 cttgtaaata ttcaggtcgt aaatctgtgt ggaaagaagg gaaacggcac cttcaggtaa
     2641 ctgtctgctg taacgaccgg tctgaataat tccggccagc tcatccagcg tcttcctggc
     2701 cagcgcggta tagtccatac agttaaccag cgtgcgtact ttaggcaggg gtatcccaag
     2761 ttcacggtag ggcagcaggt tctccatgac gttgacagta ccgcgaataa attcacgaac
     2821 atcgggaaga accggattga ccatcccgac aaccgactca gtcgccgaca gcactatcat
     2881 ctccagcatg acggagcgcg ccccctgaga atcaacgaca atgacatcgt agttattcag
     2941 gaaaagtgga tgttgcagaa cattccggag gcgaaggcgt ccgtccgggg cgtgaagcat
     3001 cgcggttttt aactgttcat gaggatcatt ggagacaatc agatcaaggc catcgatagc
     3061 cgtgcgggaa atgatattat cgggatgagt gaggtcagcc gtacgcataa gaagttcaaa
     3121 aaggccggct ggcgcttcat atgtcagcgg aaagatatta ctggctgtgg gttgagcatg
     3181 atctccgtca atcagcaggg tacgtatacc tgcatcggcc agaaaaccag caagatttgc
     3241 ggattgtgtt gatttacctt caccaccttt ggtggagaca acgggcagta ttttcattag
     3301 tacacacctc aatggtatgt aaccaactga cacgaccgtt taggacacgg ggattgaaaa
     3361 tccccgtgtc cttggttcga ttccgagtcc ggcaccacta attcttaaga acccgcccac
     3421 aaggcgggtt tttgcttttg gatctgacaa taaccttcac gaaaaaagtt tgcttataaa
     3481 gtctggggga attactctcg ccacgttaac gagagtaatt ttattgatac taatctcctg
     3541 atactttacc cccgtccaaa ctccagccgc tgcacattca ccatcccagt cttctcagca
     3601 gcactggcat caatttgtgt cacccgcagc gcatactttt catccagcgc gtttaaccag
     3661 ttcagcaggt cattaaacac caccggatct atccagacct gaatattctc cccacgctcg
     3721 gctatccgct tgatgactac cgagtgcgcg gaagcgctgt cactgataac ccgcgatacc
     3781 tgcgcaggcg ttgttgtggc ggattttcgc gctgccataa tatccggcgc ggcgctcctc
     3841 agtctggtgt tcatcgccac cagttgctgc aacattgtct cctgttgttc tatccgttcg
     3901 ctcaacggct gccagatgag gacgtaatat ccgacgctaa acaggaacac caccgctgcc
     3961 agtaacatgc ccttttcacg cggagaacgc cccgccaggt gttgtgccag ccagtgttcg
     4021 ccacggctta actggcgttc acgccattgc tgaaaatagt gaataaattt atcgcgtaac
     4081 atgttatttc ctccgcaacg ttacgccgcc ggaaaccgca tcaccctctt tctgtaatgc
     4141 gtcctgttgc acaacataat cggttgccag tgcactacgc agtttgtcga aactggcaaa
     4201 gttagcagcc cgcaactgga ggtgaagcgt ctggcgtttt tgatcaaagg tgaaaccacg
     4261 catttcgatg tcggaaagtg acgctgattt cagggtgctg gctatcgctg acaactcggc
     4321 gagcagccgg gtatcgtcgg cctgtgggcg atattttttc agcgccatcg tcacctgaga
     4381 gcgtaaattc acaatccgct tctgctccgg gaacaacgtt aagaactgtt cctccgcctg
     4441 ggtgcggctt tgcgccacct gttcgctgac gctccataac gtcacgcccc gctccactgc
     4501 cagcgcaacc agaatcagca atatcggcag aatcatcacc cgccagcgcg cccactgttt
     4561 tcggtagctg acgcgaggct gccacggccc tgtcagcagg ttcccttccg gttcgccata
     4621 agtggtaacg gcgggcagag ctgtaacggt caggcgttca gcgtctgcgc cagcccatgc
     4681 tgatagcttt tccggtgcaa tgccgactac ggttagcgaa agcggtaaat cctgctcatt
     4741 gagctgggcg cggaacatga ccggagccag cgccagcccg gcgctccatc cccggcattc
     4801 atcgatgcgg cagataaccc gttgcgcatc gccagccata aacccacaag gaatggacat
     4861 ccagtccggc gcgacggtag cgcgggtgat gccgttttcc tgcaaccact gcgcaatgtt
     4921 gcgcatatgc tgttggtgaa tcactgctac ggttgccagt tgctggtcga ttttcaacgg
     4981 ggcgaaatgc agttcatcga tatcctggtt cagctcttct tccagcaagg cgggcagaat
     5041 agtcggtatc tgcttgcggg cgacatcagg cagttcaacc tgccagacgc tgatccattc
     5101 gccgggaatg tagagtcgaa tcgcatcggt ttgcagccat tgctgaagct gttcgtcagc
     5161 aacgtcaggc cagataccgt gttccacgtc gggtgtacgg cgctgccagc ggacggtatc
     5221 ggaggcgcaa agcgggaaaa aaatctcaag gatggaactc actcactttc tcctgtctga
     5281 tgccagagaa ccgaaaagtg ttgtgggccc atgcggacaa ttaacgaatt catcgtcagt
     5341 tcaatctcat tcacggtgat atctgaacgc agccagaagt aattgctgtc cacgctcagg
     5401 acggttttta gctgtttttt agtacgctca tcgacgtcag caagtagcgg ctgtgcaaga
     5461 aactgatcga catcttccca gcccttcgcc ggacgttgtt gtaataatgc ccgcgcctga
     5521 acagggctta accacgggtc aaacagcgcc tcaagaagca cactttgcgt gacgtctaag
     5581 gtattgatgt tgatttgctg gcgggtcatc ggcagcgcac agaccagcgg tttcagtttt
     5641 tgataaagcc cgtcgtccat tccctgcacc acgcgcatct cgctgatatc ggccagcggt
     5701 tgattcgcgg cgtagaaagg caccgaacgg gcgagatact cgctgtcttc acggcccaga
     5761 cgtgtctgga cgctgcggtc ctcgtcaata aactcccaca ggctttcggc tatcagttcg
     5821 gcccgataag caggcacatc caggcgcgag atcagggcaa tcaactgttg taccgcaagc
     5881 ggacgcgacg ccgttgtcgg ctgagcaagg gcattcaggt taaagcaagc ctgtgcgtca
     5941 cgcagggtga ccgcaatttg cccctgcggc agagggaaga aacgagggcc ggatgcccac
     6001 ggctgtgtca gatgcacgcg cttttcattt ttcaggctca gactgagcgc gctcagcgcc
     6061 aggctttccg cactggcgct gtaccacagc gcctgctggt actcctgctg gctgcgcgtt
     6121 cgcccaagtt gttgctgcat ccgcccggaa agcgtgatgg ttaccagcat cataaccgcc
     6181 agcaatacca gcaccacgac cagtgccatt ccgcgttttg gtggtgaggt gatcatgata
     6241 attgcggccc gcgtaacaac cagatgcgtt caatctcgcc ccattgcggc gaatgcagag
     6301 tcatgcgcac tgccacaggg atcgcctgta ctgacgacca gctctcctgc cagcgcgtac
     6361 catcatagaa ctgcaaacgg agcgaatccg ccggaatcaa cttttgcatt gtcggcttca
     6421 cgctgcctgc ggcatcggtc agcggccagg ctaaccgttc gagataaccg tcatgaatgc
     6481 ggtaaccaac ggtgagcaga ttgctgcgtg gcagacgcat caacgggtta accacgccgc
     6541 cgcgcacaaa gcgcatgcct tcactctcag acgccagcac gccagcgccc gccagtaacg
     6601 ctggttcacg ctggccctgt tcgcctctta ccggacgcgg catcatttgt gtcagatcgt
     6661 gggtcagaaa actcatcgtt tgctgcatga ggtttagttt ttgatcatgt ccggcgacgg
     6721 cgttattcac gcgtgtgaca ccgtttgtca cctgctgcgc catcagtgcc agcgaggcaa
     6781 aaatggcaat tgccaccaac atttccagta acgtgaaacc agcgcgagtc cttctcactg
     6841 ttggcctccc acggcgctaa accaggcgcg tcgtgactga atcaccgacg aaaagtcttc
     6901 gtgacggctg acttcaatat ccaccgcctg gagcagcgcg ttgcctgtat tcaatgggat
     6961 tgttcgccag taccagcggc ttcctgccat atcgctctcg ccctgatgcc tgaccgcccg
     7021 ctgctcacgg gtcatcaggt agagcagcgc agtctggttt tcagctaccc agcccgctac
     7081 cgttttttcc tcaagaacgt gctgattact cagcgcaccg ctggcaatct gtaacaccgc
     7141 cgtggcagcc agcgcaaaaa tcgctagcgc gagcatcact tccagcaagg taaatccgcg
     7201 cttcatggca tcctctcatc acattgattg agggataacg cgccatcgtg tgccagcttt
     7261 accgcccaac atgcgttttg cgccgccgag tagaagcgca gcgtaaacgg cgtggcgggt
     7321 tcaaaaggcg aaaaacggat ctgcggagtc ttctttttcg cctctttttg cagctccagt
     7381 tcaatatcgt gcagcgtcag tcgacgacgt tgcagctcca gcgcatattc cttctgccag
     7441 atatcacttc cgggttgtaa cagcatctgc acctgtttcg gcaccgtaac ctgtgccgat
     7501 aaccgggtcg aggaaacggg cgaccactgc ccctgacgac gctgcataaa ctggtagcca
     7561 ggcgggtcga ttagcacacc gagtgttttc ccttcgatca ctgccctgtc cttaaactgc
     7621 gcaaagcgag tcagaaaatc ctgcgccgct tttttcgcgg gcgattcaga atcggtcgca
     7681 aaagtctgta ccacgcccgc actggcaagg ccgataagaa aaatcaccag catgatttcc
     7741 agaagtgtga atccgcgttc aggcactgct tactgaaact cctgcaaatt ccagttaccg
     7801 atatctgcgc cagcgccttc cccgttttcc tgaccatccg cccccagggt ataaacatca
     7861 aacagccctt tttcacccgg actgagatac tgataatcat tgccccacgg atcctttggc
     7921 agtcgtttaa tgtatccacc agtacggtag ttgcgggagt ccgccatatt ggctggctgc
     7981 tggatcagcg cctcaagccc ctgttctgtg gtcggataac gcccgttatc cagtcggtac
     8041 atatccagtg cgttttccag cgccacgata tcgctgatgg ctttttgccg atcggctttc
     8101 tctttgttgc ccaacaggtt aggcaccacc agacttgcca ggacgccaag aataacaatc
     8161 accaccatca cttccagcag ggtaaaacct gcccgtggtt tttgtgtgcg ggataacgaa
     8221 ttcatgtgat aactccgtaa attacattcc aaccatatta ttcagttgca gcatcggctc
     8281 gaggatggcg atgacaataa acagcaccac gcccgccatc atcaccacca gcgcaggctc
     8341 aaacagcccc aacgccagcc ccacctgggt atcaaattcc cgttcctggt taacagcggc
     8401 ctgctcaagc atggtttcca gttcgccgct ctgttcgccg gaggcgatca tgtacagcat
     8461 catcgggggg aacaggcgca actccgccag cgcggcgcgc agactgcttc cttcgcggac
     8521 gcgatctgcc gccagcagta gttgttgttc gacatagcga tttgccgaca cagcggcagc
     8581 ggtctgaata ccttccagta gcggaacact gctggcggtg aggatgctta aggtgcggga
     8641 aaaacgcgcc gtattcagcc cgcgcgcaac acgccctgtc acgggcagac gcagcacggt
     8701 tttatcccag cgcaggcgca tagtaggatt tttaagtagc cgttgcccca gcaccagaag
     8761 cgccagcaat cccgccagcc agtaaacgcc gctggcctgt aacgcgtcgc tcatggcaat
     8821 aagcgtgcgg gttgaggcgg gtagcgcgtg tccgagatga tcaaactgtt cgatgatttt
     8881 cggcaccact gccgtcagca aaatagtgac tacgcccgtt gccaccacca gcagaaccag
     8941 cggatagagc atggcctgca atagacgtga tttcaggcgc tgtcgctgtt cggtgtaatc
     9001 tgccaggcga ttaagcacca cgtcgagatg cccggatttt tctccggcag caaccatcga
     9061 acaaaacaga gaatcaaaga cgcggggatg ttcgcgcagg ctgtccgaca gggtgtaacc
     9121 ttcctgaatc cggctgcgca gcgccattcc gaggcttttt acatgcagtt tttcactttg
     9181 ctcactgatc gcctgtaagc aggtttccag cggcattgct gcctgtacca gcgttgccag
     9241 ttggcgcgtg aacagcgcaa gatctgccgc cgccacgcga cgatgtgcgt gccgccgacg
     9301 ctgcaacatc ccccctgacg aagtattcat ccgggcttca atatgcacgg ggataagctc
     9361 tttaccgcgc aacaactggc gggcatgacg cgcggaatcc gcctcaatca tacctttggt
     9421 tttgcgacca ttacgctcca gcgcctgata gtaaaacagt gccattacgc ctccatggtt
     9481 acccgcagaa cttcatcgag agaggtttct ccggcgagca ctttctcaat gccgttgctg
     9541 cggatacccg cagagtgttg tcggacataa cgttccagct ccagctcccc ggcctgacgg
     9601 tggatcaaat cacgcaatgt ggcatccacc acgatcagct catggatggc agtccgtccg
     9661 cgaaaacctt tgtgattaca ggcgggacag ccctgtggat ggtacagagt gacggtacgg
     9721 gcgtcggtaa ttcccagcag gcgtttttct tcgtcagtgg ctggtgcggg ctggcggcag
     9781 tcgggacaca gcgtgcgaac cagtcgctgc gccatcacgc ccgtcagact ggaagagagc
     9841 aggaaaggct ccacgcccat atcctgcaaa cgtgtgatcg cccccaccgc tgtgttggta
     9901 tgcagcgtgg aaaggaccag gtgtccggta agtgaagcct ggacagcgat ttctgcggtt
     9961 tcggtatcgc gaatttcacc gaccatcacc acatccgggt cctgacgcaa aatcgcgcgc
    10021 agcccacggg caaaggtcat gccgacgcgg gtgttaacct gcgtctgacc gatcccttca
    10081 atcatgtatt cgatgggatc ttcaaccgtg agaatgttgc gcgaatggtt gttcagctcc
    10141 tgcaatccgg cgtacagcgt ggtgcttttg ccggaacccg tcggccccgt caccagaaag
    10201 atgccgtgcg gtttgtgtaa cagctggcgc aactgcgcgg tcagttgctg gctaagcccc
    10261 agacgctcca gcgtcaggcg ggcctggttt ttgtccagca gtcgcagcac cacgcgctcg
    10321 ccccaggcag aaggcatggt ggagacacgc acgtcaatcg cccggccgcc cagcaacagc
    10381 gcaatacgtc catcctgcgg cacgcgcttt tcggcaatgt ccagccgcgc catcaccttg
    10441 atacgcgaca ccagcagcga ggccagtttg cgccccggac gcagcatttc atgtaatgtg
    10501 ccgtcaacac gaaaacggat caccagactc ttttcaaacg tctcaatgtg gatatccgaa
    10561 gcgccttctt tgattgcctc tgccagcatg gcgttgatca gtttgatgat cggcgcatcg
    10621 tcgtcgcttt ccagcagatc ttccgtttcc ggcagttctt ccgcgagggt aaaaaagtcc
    10681 tcggcagaac cgagatcttc catcagctgg cgagcctcgg aagagtcccg ctgccagacc
    10741 gcattcagcc gctgttcaaa ttcggcctcg tcgatttgcc gcagcgtaaa gggcgcgttc
    10801 agcccccgct gcagctcctg caaaacagag agcgacaacg gatgaacatg gaggatctcc
    10861 agcgacgctt cgcaccatgc caccaggcta aaccgacggc tgaaactgta gggcagacgc
    10921 acggtgttag cggtggtttc ctgtgctaca ggcaccatta acgcgttctc ccggcattaa
    10981 ggaacgcacg aacttccggc ggtagagcct gattttgcgc tggcagtacc ggctgcgcgg
    11041 tgtgcggcat caggcttaaa ccttgctcat cacgatagat ctgttcggcg cgcatatagt
    11101 tatatttgcg ctgcgacacg ccgtctgccg ccataccgtc acgcagaatg gtcggacgga
    11161 taaacaccat caggttacgt ttttcttttt tatccgccgt cgatttaaac aggttaccaa
    11221 tcaatgggat atcgccaagc agcggcactt tcgccacgct ttctcccgcc tggtcgtcca
    11281 tcagaccgcc aagcacaatc agctcaccat cgttagccag cacggtggtt ttcagtttgc
    11341 gctcaccaaa caccacgtcg aggctggtct gtccttccac cttcgagact tcctgctcaa
    11401 tcaccatctg taccgcgtta ccttcgttaa tctgcggcgt gactttcagc atgatgccga
    11461 cttttttcct ttctactgta ttgaaaggat tgctgttatt ggagccaacg gtagagccgg
    11521 ttaataccgg aacgtcctgg cccaccatga agaaggcttc ctggttgtcc agcgtggtga
    11581 tgctcggcgt ggagagcacg ttagagctgg agtcgttttt aaccgcctgc accagcgcca
    11641 tccagtcgcc tttcaccaca ccaaccgccg taccgctaaa gccggaaaga agctgggcga
    11701 gtgtggagag atcgccgttg gtatccggat taatggtggt agcgccgttt tcactgatca
    11761 ccgtcgagcc tttctgcggt ttcgcctgag aaatggctgc ccccagcgta ccgataggga
    11821 tctgcgtacc attagcaaac tgcattaatc cggcatcttt cgacgcccac tgcacgccga
    11881 agttgatatt gctaccttcg gcaacttcca cgatcaacgc ctcgacgtgc acctgagcac
    11941 ggcgaatatc cagttgttca atcacgcttt gcagcgactg catgatgtcc tgcggtgcgg
    12001 ttacaatcag ggcattactg tgtttgctgg cggcgatgga tacaatctca cgcccgctac
    12061 cgaccgtgcc ttccgcctct tctttagccg ccgtgagcgt gccgctgacc tgcttcagca
    12121 catcgaccag ctcttcggct ttgctgtatt tgagatagaa cacctggctg ttgccgctgc
    12181 gttccatttc tgagtccagc cgacggatca ggcggcgcat tttgtcccgc gtggccgggt
    12241 caccactgac aatcacactg ttggtgcgtt cgtcggcgac aatttgagat ttcagcgtcg
    12301 caggctggtt ctcgccgctg tttttcgtca ggctttccag cacgcgggcg atttccgaag
    12361 ccgaggcgtt atccagcggg atcacctctt cggtgcgatt acccgcgtga tccacgcgct
    12421 ggatcacttc tgtcaggcgc tccacaacgg aggcgcgtcc ggtgagcata atcacgttgg
    12481 agggatcgta attaacaaca ttgcctgagc ctgcgctgtc gatcatctgg cgcagaatcg
    12541 gtgccaactc gcgtaccgac acattacgta ccggcactac tttggtgacc atttcatcgc
    12601 ccgcgtagtt gtcgctgcct tcaccgacca gcggcagcgg ctcgactttc gcagcgctgg
    12661 atttcaccac cttcagcacg tcgttttcca tcggtacgac ggcatacccc tgtgcttcca
    12721 gcaggttaag gaatagctgg taatactggc gttcattgag cggggtcatg gtgcgaatac
    12781 tcactttccc ctgtacgccc ggccccataa tgatggtttt attaaggtta gcgccgacgg
    12841 tttcgatgaa cgatttcagg tcggtatctt taaaattagc ggtgaaagtg gcttcttccg
    12901 cccagaccgg tgaactgcat aatgccgctg ccagcaccag cggcagtaaa cgcttttttg
    12961 ttttgaggcc agttgtcttc ttacgccaga ccgacaacgt aatatcacgc caaaacacga
    13021 tgaatgattc tcctggatta aatgcggtta gcgcagcgcg atggaaatgt cgtggcgcgc
    13081 acccttgcgt aaaaccgtaa gttgaatgga atccattgaa ggtaactgcc gcatcagagc
    13141 aatcattgct cgtggatcag tgaaatcctg ctgatttagc gcaatggcga tatcgccttc
    13201 cttgaaaccg ctggcatcga acagagaacg atctgccccc ggcttcactg cataaccgac
    13261 aattccctcc ttacgcacag gcgtaagctg gatatagtta aaaattttct gcggatcttt
    13321 cgccagtgcc tgacgcacgg cagtcggtat ctcaactggc gcactgacag caggttcagc
    13381 aacagcttgc tttgcttcgt cactgacagc ttttttgttg gtcgcggcaa cggtggaacg
    13441 ctcctcttca gccaggctca ggcgctctat ttttccctga tagcgcagca tcacatggtc
    13501 gcgattgatt tcctcaatca ccgcattgtg cgagccaagc gtttcaccct gcaaatagac
    13561 ctgctgttta ccgccttctt caataaccgc gccgggtctg gcaccaaagg caatcccgcg
    13621 cagcaccaca ttaagacgcg tttctgccac aggcacaggt tcgggttgtt ttaccggcgt
    13681 ggcgacgggc tgatatttgc caaaccagtt ttgctggctg attaattgca cgtcattttt
    13741 atcgaacgtt tttgcatccg cacggggcgg tttattcgcc gatggggaaa cagccgtata
    13801 ttccgcagaa aaggagaaat agcgccagag tgaatgcgcc acttttgcag aaataataag
    13861 cagcatcagc cagaacatcc cgcgtacaat ttttcgcaga tgctctttgt ctgtattaag
    13921 agattgtctc tggttaagag tgtgacgaat ttttgtcagc cattgaatga gataaattcg
    13981 tgcgtcacga aaaacaaccc gcgccaattt atatctaccc gacgttatgc tttgactatt
    14041 ccacaggtgg tacgatccag tttccgctga tactggttac ctgtacgtgt attattaatc
    14101 gttatgctgt aattaattcc ctcagtgagc attaatttca ctgttgggtc agcgcacatt
    14161 tgtcgttgat agctgattaa aaaggcgtct ggcgtctgcg tggtctgagt acccgcttcg
    14221 ctgataatgg tcatttttac cgtcgtgccg ctactttgtg ccagcactaa ggtatatccc
    14281 gcagatttaa tcggcaggtt ttggctgata ttttgcgcct gttttttcgc cagcaaactg
    14341 gcgttttcat tatggctggc acagccactt aataatcctg taacgctcaa caatagcgat
    14401 attaataccc tccctggcat ctgttttatc gacatggtaa ataatctcta aggttattaa
    14461 taagagttaa aatgtcactt tgataatgac gtggttatca ttaaaacaat gcctgtagat
    14521 aaagtgttgc tataccgccc agacttaaac acggtccaaa aggcagtgtg gttgatcctt
    14581 tttttataat aacggcatat atcaggccgc agcatgaggc gattaaagca acattgggca
    14641 gtgacaacgg ccccacccaa ctacctaacg cagcgaacaa taatacatcg cccatgccta
    14701 atgcttcttt acgcagaact attccggcta tccagcgcag agagtaaaaa gtgataaacc
    14761 ccaccaggac gccggtgact gcatcttgta gcgtgagcgg gctctgctgc gcccatgccg
    14821 caattagtcc cgtccacaat acgccctgag taaaaacatc gggcagccat tggtgatcga
    14881 ggtcaatgac gctcgcggca atcagccagg cggataatat catcaccgcc agcgcccatc
    14941 cactttccgg ccagaccaga ctcgccagca aaaaagcgag tgctgtcaat aactccacca
    15001 gcggataacg cttgctgatt ttcgcctgac agtcgcggca gcgccctttg agcatcagcc
    15061 aggagagcag cggaatattg tcacgtatcc ggatggtctg ctgacaatgt gggcagtgcg
    15121 aacgcggtag cgcaaggctt atttttgact gcgcactcgg catttcacca tgaaactccg
    15181 ccatttgttg gcgcagcatg atggggtaac gccaaatcac cacattcaaa aaactgccga
    15241 taatcaatcc tccgacggta gccagtatgg gcatcgccgc ggggtattgc tgaaaaacat
    15301 caaaaagcat ggttaaaggt tgtttgttgt aacttgccgg atgcgggcct gcaggtgtat
    15361 gccatatggc tttcctccag gcccaataag ctggcgcaac ccggtgcgcc ttatttcatg
    15421 ccggatgcgg cgtgaacgcc ttatccgggt tattcagcag gcatcgaata ctcggtaacc
    15481 ttattaatgg tttccggccc ttgcttcggt ttcggcagat tgagtgacgc cagcgtgttg
    15541 tacgccgact ggctcacacc gccctcgaag ctcatctcgc tcgcccccgg cagctggtaa
    15601 gcattcgcac ccggattcca tttcttaaag aactccgaaa gatccgtctg agcgacccag
    15661 gatgcacaca gcatcagcgt gtcagcagcg ttaccgttgg attcagcaca gtaattcttg
    15721 ccaccaaagg tcttgtcacc aacgtcatcg ccgcgcgctt tacggtgcat caactggaac
    15781 aggttccagc ctttcatccc ttcacgctcg ctgtaaaact ctggcagagg agtgccatct
    15841 ggataccatt tcttgatatc aaagtttttc tctgcccatt ccttcagctg cgcgtacatc
    15901 agcagacggt cacccgcacc gccgcgtgcc catgcctggt tgttgctctc ctccagatat
    15961 tccggtgcga cggtaatatc gtcagcgaca cggttcatct tgccgagata gcgatcctgc
    16021 atgtacagcg ccagcacgtt gttcgcgact tcagttgcac ccggtacagt cagcggtgtt
    16081 tctgcggcgt tgtgccctac ttcgtgccag atcaaccagt cgttcagcgg cgtcgtcggc
    16141 agcgtggtgc tgttcgtcga gaagctgctg ttcattaccg gataacccga gtgcgcatca
    16201 ccgatggaga tctgcacatc gtttgtgaaa cgatgtttat gtcccgtcaa tgctttatag
    16261 gtaaacatcc ggtgcttacc gctcgtttca tcacgaccgt agaagtcatt catcgatctg
    16321 gcaaaggtgt ccagatcgtt agcgaattgc tccagtccgc cagtgtaatt gctggcattc
    16381 aggttcttct tcggtgtggt atagacgaaa gcgtctgatt ccagctcacc cagcggagcc
    16441 ggtgagttca gatcgttttt ccatgcgccg tctttataga acggtgcttt taccacgcca
    16501 gtaaaggtga agctggcaga ttcattggta gagctattgc ccttgatata aatcaggcca
    16561 ccgtaaggca ccttgaactt caccgtaccg ctagcgtcca gagagtacgt tttagtcact
    16621 cttggcggac ggttcagcgc aacttcatgc ttctcacgtc cggtcaggtc gtcagccagc
    16681 gccacggtga cggtcacagg aacgttcgca ttggacttaa tggtgacctc tttctgagcc
    16741 ggtgcccaca ggccagttga ctgcatgtta cctgcaaacc atttggtcgg attcgagtac
    16801 aggctgatgg tttcagtaac gttctgtccc tcttccgata ctgctcccgg atacttctcg
    16861 acatcaactt tgatgttcag atcccaccag gaacggccca gcatcaggcg tgtcagcggt
    16921 ttttccatat agttgagcgg gtagctcggg ttcatcatgc ccgctttgct gctaccgtca
    16981 ccgtagatca tgttgttatc gaccagcgat tttttcagat ctgcagaaca cttggtgccg
    17041 cctgcatagg catcattggc gtagcagttc aggaactcgg tgaacgtttt aaagcccagc
    17101 tcgtcatttt tgccttcttc ataacgatag ctcgtatcgt tccacagcca gaccgacatg
    17161 ttctggtaca gacgttccag atcgacgctg ctcagacgct cacctttgcg accattggtc
    17221 cggaagtaga gctcatgctg atacagacgc tgaatgttgg tgcctaaatc cgcagcctgc
    17281 accatcgctt ttgcagtgtc ggcgttaagg cttagttgcg tatactgtgg aacatacatg
    17341 ccaccagtaa ccggaacccc cgtgccagga cgatattcca gacagttgac ctcatagtga
    17401 tatgccggat tagtacactc tttcagcccc gggaacgcgg cgaaaatttt ctccttcgca
    17461 gccttcagag aatcctctgt tttatgatcg gcctcatcaa taaaggcata acgcgtttcc
    17521 tgtttgccat ctacatcttc cagccagctg gcaacttcca gcttcggttt gtcatcaggt
    17581 ttgttttcta cctgatattt ccacttaact tcccctgtct tactatcgat ggtgtacggc
    17641 agcgcaccat ctacggcagg ataacgttca tagacccaaa tgcccgttgc gcgctgctga
    17701 cgaacgcggt tcggataccc ttgcggatcg ttatttacta ccgacttgtt cagtgccatc
    17761 gacagacctg cggcatccaa cagacgcaca aaaccagacg cgctctcttc cttaagattg
    17821 ctcatcacgt tttccatgat cagcaccgat ccacctttgt tcagataggc gatcagatcg
    17881 gtcacatcct gctgagtcag cttcggtttg ctggtatctg cacgcagcgg gattgcataa
    17941 ggatcgttac ccacctgagt cacatattca aagccgttaa ggatcagcag cggcatttcc
    18001 tgcggatcga gatcgccata gctacttaaa tgctcaacag agatgcccgc aaaatccgga
    18061 tggaagtcga acgcagcgct gtttcctgta acctgaccat gacgtttaaa atagacagta
    18121 tccaggttgg tgcctacggt catgctggct ttcgcgtccg gcgtccattt atcgtcggac
    18181 agatagcgca gcacgttctc catgaagttc ttcatgtcat ccgggtcgct gttgagcgta
    18241 cactgcccat ctttattaac gcccccgttc cagctgtaac cgttcgggca acgcaaaatg
    18301 ctgttgtagt gtgggttacc gataaccatc agtttgccct caccgacttg ccccagcgaa
    18361 ataaacggca ggttgaaagt ggcggtatcg cgcgtaacgt tttccggctc aacaagagaa
    18421 ggcgcttccg taatgtacgc cagctcattt ttatcccagg cgcgtttttc gccaaacgcc
    18481 agccagtagt ttttatcatt acgcgccatc agaatcggga aggccgcgtt ggagatattc
    18541 accaccgcct gaccgcgcgc attacccgtg ctgccataga agttggtaga gtcatggaat
    18601 acatggaact tgctgacaga tttgtagttc gtatccacgc cccacagctt gttgataacg
    18661 ccctgaatct ggccgtcatt aacattgcgc gtcgtcagcg agaaccagcg agcctcgtta
    18721 caaccgtcgg ttttcgcaca aatcgcggta tcgatctctt tggcctgacc cgtcttaaac
    18781 tgctcgataa attcgttagg cagattaacg acttgctcac cttcccccag cgtcgcacca
    18841 ttggataacg agagattgat aatctcgtta atcacgttgg gatattcagc aaaaaccttg
    18901 cgcacttcat ccggaactac acgcgtgtga ttttgtccag ccttcgaata gcggtgaatc
    18961 aactgatcga tatttgcccc gcgaacttca tcacccagtt cagtcaatgc aatagtcgac
    19021 ttgttaccac gcacagaacc aagctcaaaa gtgtcgatgc caaaggagat ggtttccccc
    19081 caactaaagg aaaattcccc gttttctccg gttacaccac ggccggaatt ggtgtaatag
    19141 ttgacaccaa caacaccatc cccctgacta tcgaccagtc gaccttcaga gagaatgatt
    19201 tcagtgggtt gatactgata aaactgttcc gcgttagccg acacgaagga agcgtttaga
    19261 tccggttttg ttcccggagt ggtggcgggc acgaccggtg aagtatgagt ggatggcgct
    19321 ttatcggtcg cggcattgtt ttccacctct tcattgacca gctttttgaa ttcttccggt
    19381 gccagatcga tttgcttata cagcgagtcg aaacgtttac tctcgatcac cgaggagaac
    19441 tccaggcaca cctgttctgt attcgccggg caactgttca tggaggtgac cagcgagagc
    19501 gcattgcttt tcttgttgtc ggaacccgcc agttcttgcg cgtcctcaag actaaacgac
    19561 actttttcaa ccgcacgcag gctacgcgca gcttctgact gggtgtcgaa ggtagcaatt
    19621 gtcgtgttgt tccctgccac acaggtgact ttgtcgcctg gtgtaaaggt aaagccatcg
    19681 ctggattcat cattacaggt gatatcacca gttacccgca ggcttccgcc cagagtcaga
    19741 taacccgttt tcgtaggaac aggttctggc tccggttctg gtgttggctc aggatcaggt
    19801 gtcggttccg gcgtaggttc tgggtccggc gttggctcag gcgtcggctc cgggtttggt
    19861 gttggatcag gtttcacttc cggcaaagac cctgttccag aatctacagg cggcgtatcg
    19921 gaggaagat
//
LOCUS       DAZFQD010000097        19922 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.097, whole genome shotgun sequence.
ACCESSION   DAZFQD010000097 DAZFQD010000000
VERSION     DAZFQD010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19922
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.097"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(260..466)
                     /gene="ybfA"
                     /locus_tag="ACHKTW_003434"
     CDS             complement(260..466)
                     /gene="ybfA"
                     /locus_tag="ACHKTW_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HFO6464390.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
     gene            779..868
                     /gene="kdpF"
                     /locus_tag="ACHKTW_003435"
     CDS             779..868
                     /gene="kdpF"
                     /locus_tag="ACHKTW_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HFO6464391.1"
                     /translation="MSAGVITGVLLVFLLLGYLVYALINAEAF"
     gene            868..2541
                     /gene="kdpA"
                     /locus_tag="ACHKTW_003436"
     CDS             868..2541
                     /gene="kdpA"
                     /locus_tag="ACHKTW_003436"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HFO6464392.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTTGVER
                     VLFRALGVSDREMNWKQYLCAILGLNMLGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFALIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALLIALFFIQQGALQNFQPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPGP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            2564..4612
                     /gene="kdpB"
                     /locus_tag="ACHKTW_003437"
     CDS             2564..4612
                     /gene="kdpB"
                     /locus_tag="ACHKTW_003437"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HFO6464393.1"
                     /translation="MSRKQLALFEPTLVVQALKEAVKKLNPQAQWRNPVMFIVWIGSL
                     LTTCISIAMASGAMPGNALFSAAISGWLWVTVLFANFAEALAEGRSKAQANSLKGVKK
                     TAFARKLREPKYGAAADKVPADQLRKGDIVLVEAGDIIPCDGEVIEGGASVDESAITG
                     ESAPVIRESGGDFASVTGGTRILSDWLVIECSVNPGETFLDRMIAMVEGAQRRKTPNE
                     IALTILLIALTIVFLLATATLWPFSAWGGNAVSVTVLVALLVCLIPTTIGGLLSAIGV
                     AGMSRMLGANVIATSGRAVEAAGDVDVLLLDKTGTITLGNRQASEFIPAQGVDEKTLA
                     DAAQLASLADETPEGRSIVILAKQRFNLRERDVQSLHATFVPFTAQSRMSGINIDNRM
                     IRKGSVDAIRRHVEANGGHFPTDVDQKVDQVARQGATPLVVVEGSRVLGVITLKDIVK
                     GGIKERFAQLRKMGIKTVMITGDNRLTAAAIAAEAGVDDFLAEATPEAKLALIRQYQA
                     EGRLVAMTGDGTNDAPALAQADVAVAMNSGTQAAKEAGNMVDLDSNPTKLIEVVHIGK
                     QMLMTRGSLTTFSIANDVAKYFAIIPAAFAATYPQLNALNIMRLHSPDSAILSAVIFN
                     ALIIVFLIPLALKGVSYKPLTASAMLRRNLWIYGLGGLLVPFIGIKVIDLLLTVCGLV
                     "
     gene            4621..5193
                     /gene="kdpC"
                     /locus_tag="ACHKTW_003438"
     CDS             4621..5193
                     /gene="kdpC"
                     /locus_tag="ACHKTW_003438"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HFO6464394.1"
                     /translation="MRGLRPALSTFIFLLLITGGVYPLLTTVLGQWCFPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFQGRPSATAEMPYNPQASGGSNLAVSNPELDKQIAARV
                     AALRAANPDASTNVPVELVTASASGLDNNITPQAAVWQIPRVAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            5186..7870
                     /gene="kdpD"
                     /locus_tag="ACHKTW_003439"
     CDS             5186..7870
                     /gene="kdpD"
                     /locus_tag="ACHKTW_003439"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
                     /protein_id="HFO6464395.1"
                     /translation="MNNEPLRPDPDRLLEQTAAPHRGKLKVFFGACAGVGKTWAMLAE
                     AQRLRAQGLDIVVGVVETHGRKDTAAMLEGLAVLPPKRQAYRGRHISEFDLDAALARR
                     PALILMDELAHSNAPGSRHPKRWQDIEELLEAGIDVFTTVNVQHLESLNDVVSGVTGI
                     QVRETVPDPFFDAADDVVLVDLPPDDLRQRLKEGKVYIAGQAERAIEHFFRKGNLIAL
                     RELALRRTADRVDEQMRAWRGHPGEEKVWHTRDAILLCIGHNTGSEKLVRAAARLASR
                     LGSVWHAVYVETPALHRLPEKKRRAILSALRLAQELGAETATLSDPAEEKAVVRYARE
                     HNLGKIILGRPASRRWWRRETFADRLARIAPDLDQVLVALDEPPARTINNAPDSRSFK
                     DKWRVQIQGCVVAAALCAVITLIAMQWLMAFDAANLVMLYLLGVVVVALFYGRWPSVV
                     ATVINVVSFDLFFIAPRGTLAVSDVQYLLTFAVMLTVGLVIGNLTAGVRYQARVARYR
                     EQRTRHLYEMSKALAVGRSPQDIAATSEQFIASTFHARSQVLLPDDNGKLQPLTHPQG
                     MTPWDDAIAQWSFDKGLPAGAGTDTLPGVPYQILPLKSGEKTYGLVVVEPGNLRQLMI
                     PEQQRLLETFTLLVANAFERLTLTASEEQARMASEREQIRNALLAALSHDLRTPLTVL
                     FGQAEILTLDLASEGSPHARQASEIRQHVLNTTRLVNNLLDMARIQSGGFNLKKEWLT
                     LEEVVGSALQMLEPGLSSPINLSLPEPLTLIHVDGPLFERVLINLLENAVKYAGAQAE
                     IGIDAHVEGENLQLDVWDNGPGLPPGQEQTIFDKFARGNKESAVPGVGLGLAICRAIV
                     DVHGGTITAFNRPEGGACFRVTLPQQTAPELEDFHEDM"
     gene            7867..8544
                     /gene="kdpE"
                     /locus_tag="ACHKTW_003440"
     CDS             7867..8544
                     /gene="kdpE"
                     /locus_tag="ACHKTW_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HFO6464396.1"
                     /translation="MTNVLIVEDEQAIRRFLRTALEGDGMRVYEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSAVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATAAPDPLVKFSDVTVDLAARVIHRGEEEVHLTPIEFRLL
                     AVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMGHLRQKLEQDPARPRHFITETG
                     IGYRFMP"
     gene            8706..8840
                     /locus_tag="ACHKTW_003441"
     CDS             8706..8840
                     /locus_tag="ACHKTW_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001417766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HFO6464397.1"
                     /translation="MIYYLGYDRFLLIFFTDYSHFMDRITLPSTDTARGKGKWKIITA
                     "
     gene            8819..8923
                     /gene="speFL"
                     /locus_tag="ACHKTW_003442"
     CDS             8819..8923
                     /gene="speFL"
                     /locus_tag="ACHKTW_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leader peptide SpeFL"
                     /protein_id="HFO6464398.1"
                     /translation="MENNNRLMPHIRRTTHIMKFAHRNSFDFHFFNAR"
     gene            9234..11432
                     /gene="speF"
                     /locus_tag="ACHKTW_003443"
     CDS             9234..11432
                     /gene="speF"
                     /locus_tag="ACHKTW_003443"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004586 - ornithine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
                     /protein_id="HFO6464399.1"
                     /translation="MSELKIAVSRSCPDCFSTHRACVNIDESNYIDVAAIILSVSDVE
                     RGKLDEIDATGYDIPVFIATENEERVPAEYLPRISGVFEHCESRKEFYGRQLETAASH
                     YETQLRPPFFRALVDYVNQGNSAFDCPGHQGGEFFRRHPAGNQFVEYFGEMLFRSDLC
                     NADVAMGDLLIHEGAPCIAQQHAAKVFNADKTYFVLNGTSSSNKVVLNALLTPGDLVL
                     FDRNNHKSNHHGALLQAGATPVYLETARNPYGFIGGIDAHCFEESYLRELITEVAPQR
                     AKEARPFRLAVIQLGTYDGTIYNARQVVDKIGHLCDYILFDSAWVGYEQFIPMMADCS
                     PLLLDLNENDPGILVTQSVHKQQAGFSQTSQIHKKDSHIKGQQRYVPHKRMNNAFMMH
                     ASTSPFYPLFAALDINAKMHEGVSGRNMWMDCVVNGINARKLILDNCQHIRPFVPELV
                     DGKPWQSYETAQIAVDLRFFQFVPGEHWHSFEGYAENQYFVDPCKLLLTTPGIDARNG
                     EYEAFGVPATILANFLRENGVVPEKCDLNSILFLLTPAEDMAKLQQLVALLVRFEKLL
                     ESDAPLAEVLPSIYKQHEERYAGYTLRQLCQEMHDLYARHNVKQLQKEMFHKEHFPRV
                     SMNPQEANYAYLRGEVELVRLPDAEGRIAAEGALPYPPGVLCVVPGEIWGGAVLRYFS
                     ALEEGINLLPGFAPELQGVYIEEHDGRKQVWCYVIKPRDAQSTLLKGEKL"
     gene            11429..12748
                     /gene="potE"
                     /locus_tag="ACHKTW_003444"
     CDS             11429..12748
                     /gene="potE"
                     /locus_tag="ACHKTW_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015496 - putrescine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:0015847 - putrescine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HFO6464400.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFMANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGVIIP
                     VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(12804..14444)
                     /gene="pgm"
                     /locus_tag="ACHKTW_003445"
     CDS             complement(12804..14444)
                     /gene="pgm"
                     /locus_tag="ACHKTW_003445"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004614 - phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase
                     (alpha-D-glucose-1,6-bisphosphate-dependent)"
                     /protein_id="HFO6464401.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAISNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALMADGLKGVKRISLDEAMASGHVKEQDLVQPFVEGLADI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(14470..15015)
                     /gene="seqA"
                     /locus_tag="ACHKTW_003446"
     CDS             complement(14470..15015)
                     /gene="seqA"
                     /locus_tag="ACHKTW_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032297 - negative regulation of
                     DNA-templated DNA replication initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HFO6464402.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            15200..15964
                     /gene="ybfF"
                     /locus_tag="ACHKTW_003447"
     CDS             15200..15964
                     /gene="ybfF"
                     /locus_tag="ACHKTW_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFO6464403.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPRDPVMNYPAMAQDLVDTLDAQQIDKATFIGHSMGGKAVMALTALA
                     PDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            16104..16397
                     /gene="ybfE"
                     /locus_tag="ACHKTW_003448"
     CDS             16104..16397
                     /gene="ybfE"
                     /locus_tag="ACHKTW_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HFO6464404.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            16537..17067
                     /gene="fldA"
                     /locus_tag="ACHKTW_003449"
     CDS             16537..17067
                     /gene="fldA"
                     /locus_tag="ACHKTW_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HFO6464405.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            17277..17363
                     /gene="uof"
                     /locus_tag="ACHKTW_003450"
     CDS             17277..17363
                     /gene="uof"
                     /locus_tag="ACHKTW_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HFO6464406.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            17356..17802
                     /gene="fur"
                     /locus_tag="ACHKTW_003451"
     CDS             17356..17802
                     /gene="fur"
                     /locus_tag="ACHKTW_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HFO6464407.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(17886..18212)
                     /gene="chiQ"
                     /locus_tag="ACHKTW_003452"
     CDS             complement(17886..18212)
                     /gene="chiQ"
                     /locus_tag="ACHKTW_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChiQ/YbfN family lipoprotein"
                     /protein_id="HFO6464408.1"
                     /translation="MKKLILIAIMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQQFADQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(18262..19668)
                     /gene="chiP"
                     /locus_tag="ACHKTW_003453"
     CDS             complement(18262..19668)
                     /gene="chiP"
                     /locus_tag="ACHKTW_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
                     /protein_id="HFO6464409.1"
                     /translation="MRTFSGKRSTLALAIAGVTAMSGFMAMPEARAEGFIDDSTLTGG
                     IYYWQRERDRKDVTDGDKYKTNLSHSTWNANLDFQSGYAADMFGLDIAAFTAIEMAEN
                     GDSSHPNEIAFSKSNKAYDEDWSGDKSGISLYKAAAKFKYGPVWARAGYIQPTGQTLL
                     APHWSFMPGTYQGAEAGANFDYGDAGALSFSYMWTNEYKAPWHLEMDEFYQNDKTTKV
                     DYLHSLGAKYDFKNNFVLEAAFGQAEGYIDQYFAKASYKFDIAGSPLTTSYQFYGTRD
                     KVDDRSVNDLYDSTAWLQALTFGYRAADVVDLRLEGTWVKADGQQGYFLQRMTPTYAS
                     SNGRLDIWWDNRSDFNANGEKAVFFGAMYDLKNWNLPGFAIGASYVYAWDAKPATWQS
                     NPNAYYDKNRTIEESAYSLDAVYTIQDGRAKGTMFKLHFTEYDNHSDIPSWGGGYGNI
                     FQDERDVKFMVIAPFTIF"
BASE COUNT         4569 a         4989 c         5287 g         5077 t
ORIGIN      
        1 gccggttttc cgctcatctg ctcatccttg ttttatcggg ttatttgtct gcttccaggc
       61 aaaaccctac agatttataa gaaagcctta agtgagatat ccatcagata gaaacaatta
      121 ggaaaatatt tatatatatt tcatgaatat atgtttcgat tggtataacg atgtctaaat
      181 attcctctga acatcttcct gatacaaaaa accgccagcg tttccgctgg cggttttcaa
      241 tgtattcaaa actcagcaat caataaaaat caccagttgc cttttcggcc tgatccatcc
      301 acaccggttt atcgctggtt ttcgaccaga cgcgatgcag gtagctgtaa aagcgggcgc
      361 ggtctttcca gaaaagcatg aaaggcagcg ccagaacgcc cgctgcaacc gcataagtac
      421 ggcgtaaaaa gataagccat gcaggatatt ctctgtagag ttccatactt ttctccccct
      481 tttgtatcta cccggtgaat ggcaccggaa aaatgaattt gtttatctga tgaaaatagt
      541 accgcctttt gtgtaatttt actactcatc cgaccactta tttttgctta ttgatggttt
      601 atttacattc atcctgtaat taagttacac aaaagttaaa ttaatactaa acattagtta
      661 aatcatggct tttgccattt ttatactttt tttacacccc gcccgcagat ttttgcgaaa
      721 tctttgcagc cagaattcta cccttccggt atcactttta ggccactgga ggtgcactgt
      781 gagtgcaggc gtgataaccg gcgtattgct ggtgttttta ttactgggtt atctggttta
      841 tgccctgatc aatgcggagg cgttctgatg gctgcgcaag ggttcttact gatcgccacg
      901 tttttactgg tgttaatggt gctggcgcgt cctttaggca gcgggctggc gcggctgatt
      961 aatgacattc ctcttcccgg tacaacgggc gttgagcgcg tactttttcg cgcacttggc
     1021 gtctctgacc gtgagatgaa ctggaagcaa tatctctgtg ccattctcgg cctgaacatg
     1081 ctggggctgg cggtgctgtt ttttatgttg ctcggtcagc actatctgcc gcttaatcca
     1141 cagcagttgc cggggctgtc gtgggatctg gcgctgaata ccgccgtcag ctttgtcacg
     1201 aataccaact ggcaatctta cagcggtgaa accacgttga gctatttcag ccagatggct
     1261 ggcttaacgg tgcaaaactt tctttctgcc gccagcggga ttgcggtgat ttttgccctc
     1321 atccgtgcgt ttacccgcca gagcatgagc acactcggga atgcctgggt cgatctgcta
     1381 cgcatcacgt tatgggtgct cgtccctgtg gcgttgttga tcgcactgtt ttttattcaa
     1441 caaggtgcgc tgcaaaactt tcagccttat caggctgtga ataccgttga aggagcgcaa
     1501 cagctgttac ccatggggcc tgtagcttct caggaagcga tcaagatgct cggtactaac
     1561 ggcggtggct tctttaatgc caactcgtcg catccgtttg aaaacccaac cgcactgacc
     1621 aacttcgtgc agatgctggc gatcttcttg atcccaacgg cgctgtgctt tgcctttggt
     1681 gaagtgacgg gcgatcgccg ccaggggcgc atgttgctgt gggcgatgtc agtgattttt
     1741 gtcatctgcg taggcgtggt gatgtgggca gaagttcagg gtaatcctca tctgctggcg
     1801 ctcggcgcag acagcagcat caatatggaa ggtaaagaga gccgtttcgg cgtgctggtc
     1861 agtagcctgt ttgcggtcgt gactacggcg gcttcctgtg gcgcggtgat tgcgatgcat
     1921 gattcgttta ccgctctcgg tggcatggtg ccgatgtggc tgatgcaaat tggtgaagtg
     1981 gtgttcggcg gtgtcggttc tggtctttac ggcatgatgc tgtttgtcct gctggcggtg
     2041 tttattgccg ggctgatgat tggtcgtaca ccggaatatc tgggtaaaaa aatcgacgta
     2101 cgcgagatga aactgactgc actggcgatt ctggttaccc cgacgctggt gctgatgggc
     2161 gcggcgctgg caatgatgac cgacgccgga cgtagcgcca tgctcaaccc tggcccgcat
     2221 ggttttagcg aagtgctgta cgccgtgtca tccgccgcta acaacaacgg cagcgccttt
     2281 gccggattaa gcgccaactc tccgttctgg aactgtttac tggcgttctg catgtttgtc
     2341 ggtcgcttcg gggtgattat cccggtgatg gcaattgccg gttcgctggt gagtaaaaag
     2401 agccaacccg ccagctccgg cacgctgcca acgcacggcc cgctgtttgt tggcctgtta
     2461 atcggcaccg tgttgctggt tggcgcactg acctttatcc ctgccctggc gcttggtcca
     2521 gtggcggaat atctctcctg atgatattga gtgagcactg aatatgagtc gtaaacaact
     2581 ggcgctattc gaaccaacac ttgtcgttca ggcgctgaaa gaagcggtga aaaaattaaa
     2641 cccgcaggcg caatggcgca atccggtgat gtttatcgtc tggatcggca gtctgctgac
     2701 cacctgtatt agcatcgcga tggcaagcgg tgcgatgcct ggcaatgcgc tgtttagcgc
     2761 ggcaattagc ggctggctgt gggtcaccgt actgttcgct aatttcgccg aggcgctggc
     2821 agaaggccgc agtaaagcgc aggccaacag tcttaaaggg gtgaaaaaaa ctgcctttgc
     2881 ccgcaagctg cgtgagccga aatatggcgc tgcggcggac aaagttcctg ccgatcaact
     2941 gcgtaaaggt gacatcgtgc tggtagaagc tggcgatatt atcccctgcg atggtgaagt
     3001 cattgaaggc ggcgcatcgg tcgatgaaag cgccatcacc ggggaatcgg caccggtgat
     3061 ccgtgaatcc ggcggcgatt ttgcctccgt caccggcggc acgcgtattc tttctgactg
     3121 gctggtgatt gagtgtagcg ttaaccccgg tgagacattt ctggatcgga tgatcgcgat
     3181 ggtggaaggc gcacagcgac gcaaaacacc gaacgagatt gccctgacta tcctgctgat
     3241 tgctctgacc atcgtctttt tactggcaac cgccacgctg tggccgtttt ccgcgtgggg
     3301 cggtaatgcc gtgagtgtaa cggtactggt ggcgctgctg gtctgtctga tcccaaccac
     3361 tattggcggc ctgttgtcag cgatcggcgt cgccgggatg agtcggatgc tgggcgcgaa
     3421 tgtgattgcc accagcggac gtgcagttga agcggcaggt gacgttgacg ttctgctact
     3481 ggataaaacc ggcaccatca cactcggtaa ccgtcaggcg tcggagttta tccctgcaca
     3541 gggcgtggat gaaaaaacgc tggctgacgc cgcacaactg gcttcgctgg ctgatgaaac
     3601 gccggaaggc cgcagtattg tgatcctcgc caagcaacgt tttaacctgc gcgagcgcga
     3661 tgtgcagtcg ctccatgcca cctttgtacc gtttactgcg caaagccgga tgagcgggat
     3721 caacatcgac aaccgcatga tccgtaaagg ttctgtcgat gccattcgcc gccatgttga
     3781 ggctaacggt ggtcacttcc ctaccgatgt tgatcaaaaa gtcgatcagg ttgcgcgtca
     3841 gggggccacg ccgctggtgg tggtggaagg ttcccgtgtg ctgggcgtta ttacgctgaa
     3901 agatatcgtc aaaggcggta ttaaagagcg cttcgcccag ctgcgcaaaa tgggcattaa
     3961 aacggtgatg attaccggcg ataaccgtct gactgccgcc gccattgctg cggaagcggg
     4021 cgtcgatgat tttctcgccg aagcgacacc ggaggccaag ctggcattga ttcgtcagta
     4081 tcaggcggaa ggccgtttgg tggcgatgac cggcgacggc accaacgatg ctccagcgct
     4141 ggcgcaggca gatgtcgcgg tggcgatgaa ctccggtact caggcggcga aagaagcggg
     4201 caatatggtc gatctcgact ctaacccgac caagctgatc gaggtggtac atattggcaa
     4261 acagatgctg atgacccgtg gctcgctgac caccttcagc attgccaacg atgtggcgaa
     4321 atacttcgcc attattccgg cggcgttcgc ggcaacgtat ccgcagttaa atgcgctgaa
     4381 catcatgcgc cttcattcgc ccgactccgc cattctcagt gcggtcattt tcaacgcctt
     4441 gattatcgtc tttttgattc ctctggcgtt aaaaggcgtg agttataaac cgcttaccgc
     4501 ttctgccatg ttgcgccgta acttatggat ttacggtctg ggtgggctgc tggtgccgtt
     4561 tattggtatc aaagtcattg atttactgct gaccgtttgc ggtctggtgt gaggtttacc
     4621 atgcgtggct tacgtccggc attatcgaca tttatctttc tgttattgat tactggcggc
     4681 gtttacccgc tgctgaccac cgtgctgggg caatggtgtt ttccctggca ggccaacggt
     4741 tcgttgattc gtgaaggtga tacggtgcgg ggttcggcat taatcgggca gaattttacc
     4801 ggcaacggct attttcaggg tcgcccgtcg gcaacggcag aaatgcccta taatccacag
     4861 gcttctggcg ggagcaatct ggcggtcagt aaccctgagc tggataaaca aatagccgca
     4921 cgcgttgctg cattacgggc cgctaacccg gatgccagta cgaacgttcc ggttgaactg
     4981 gtgacggcat cggcaagcgg gctggacaat aatatcaccc cgcaagcggc ggtctggcaa
     5041 atcccacgcg tggcgaaagc gcgtaatctc agcgttgaac agctcacgca actgatcgca
     5101 aaatacagcc aacaaccgct ggtgaaatat atcggccagc cggtcgtcaa cattgttgaa
     5161 ctcaatctgg cgctggataa acttgatgaa taacgaaccc ttacgtcccg accccgatcg
     5221 tctgctggaa caaactgccg cgccgcatcg ggggaagctg aaagttttct tcggtgcctg
     5281 tgcaggcgtc gggaagacct gggcgatgct ggcagaagcc cagcgactgc gggcgcaagg
     5341 gctggatatt gtggttggcg tggtagaaac ccatgggcgg aaagataccg ccgccatgct
     5401 tgaagggctg gctgttctgc cgcccaaacg ccaggcgtac cgtgggcggc atatcagcga
     5461 gtttgatctc gatgccgccc tcgcccgccg tccggcgctg atcttaatgg acgaactggc
     5521 gcacagtaat gcgccaggtt ctcgtcatcc taaacgctgg caggatatcg aagaactgct
     5581 ggaagctggc attgatgttt tcacaaccgt caacgttcag catctggaaa gtctgaatga
     5641 tgtggtcagc ggcgtcaccg gaattcaggt acgggaaacc gtgcccgatc cttttttcga
     5701 tgccgccgac gacgtagtgc tggtggactt gcccccggac gatctgcgcc agcggctgaa
     5761 agaaggcaaa gtctatattg ccgggcaggc ggagcgcgcc attgaacatt ttttccgcaa
     5821 aggcaatctg atcgccctgc gtgaactggc actgcgccgt actgccgatc gcgtagatga
     5881 gcaaatgcgc gcctggcggg ggcatcctgg tgaagagaaa gtgtggcaca cgcgcgacgc
     5941 gatcctttta tgcatcgggc ataacaccgg cagtgagaaa ctggtccgcg cggcggcgcg
     6001 gttggcctcg cgcctgggta gcgtctggca cgcggtgtat gttgaaaccc ctgccctgca
     6061 tcgcttaccg gaaaaaaaac gtcgggcaat tctcagcgcc ttacgtctgg cgcaggagct
     6121 gggcgcggag acggctacac tttctgatcc agcggaagaa aaagcagtag tgcgttatgc
     6181 ccgtgaacat aatctcggca agattattct cggtcgcccg gcctcgcgcc gctggtggcg
     6241 tcgggaaacg tttgctgacc gactggcgcg catcgccccc gatctcgatc aggtgctggt
     6301 cgcgcttgat gaaccacccg cccgcacgat taacaacgcg ccggatagcc gctcttttaa
     6361 agacaagtgg cgggtacaga ttcagggatg cgtggttgcc gccgcgttat gcgccgttat
     6421 cactttaatt gccatgcagt ggctgatggc gtttgatgcc gccaacctgg tgatgctgta
     6481 tctgcttggc gtggtggtgg tggcgctatt ttatggacgc tggccttcgg tggttgctac
     6541 cgtcattaat gtggtgagtt tcgatctctt ttttatcgcc ccacgcggca cgctcgctgt
     6601 ctctgatgtg caatatctgc tgaccttcgc ggtgatgtta accgtcgggc tggtgatcgg
     6661 gaaccttact gcgggtgtgc gttatcaggc gcgggtagcc cgttaccgcg agcaacgcac
     6721 acggcactta tatgaaatgt cgaaagctct ggcggtgggc cgcagtccgc aggacatcgc
     6781 cgccaccagc gaacaattta ttgcctccac gtttcatgcc cgcagtcagg tgttgttgcc
     6841 cgatgacaac ggtaaattgc agccgttaac acatccgcaa ggaatgacgc catgggacga
     6901 tgccatcgcg cagtggagtt ttgataaagg cctgcctgcg ggtgcgggca ccgacacgtt
     6961 acccggcgtg ccgtaccaga ttttgccgct aaaaagcggc gagaaaacct acggactggt
     7021 ggtggtggaa ccggggaatc tgcgtcagtt gatgatcccg gagcagcagc gcctgctaga
     7081 gacgtttacg ctgttagtcg ccaatgcgtt tgagcggctg acgctgaccg ccagtgaaga
     7141 acaggcgcgg atggcaagcg aacgtgaaca gatccgcaac gccctgctgg cggcgctttc
     7201 gcatgattta cgcacgccgc ttacggtgct gtttggtcag gcagaaatct taacgctcga
     7261 tctggcaagc gaaggatcgc cccacgcccg ccaggccagc gagattcgtc agcatgtgct
     7321 gaacactacc cgactggtga ataatctact ggatatggcg cgaattcagt ccggcggctt
     7381 taatttgaag aaagagtggt taacgctgga agaagtagtc ggcagcgcgc tgcaaatgct
     7441 ggaaccgggt ttatcgtcgc ccatcaatct ttctctgcca gaaccgctga ccttaatcca
     7501 cgttgacggg ccactctttg aacgggtgct gattaatctg ctggagaacg cggtgaaata
     7561 tgcgggtgcg caggccgaaa ttggtatcga tgcccacgtt gagggcgaaa atctacaact
     7621 ggatgtctgg gataacggcc ccggtcttcc gccaggccag gagcagacga tatttgataa
     7681 gtttgctcgc gggaataaag agtcggcagt accgggggta gggcttggac tggcaatttg
     7741 tcgggcgata gtggatgtac acgggggcac tattaccgcg ttcaaccgac cggaaggtgg
     7801 tgcctgtttt cgtgttacac ttccccagca aaccgctcct gaacttgaag actttcatga
     7861 ggatatgtga caaacgttct gattgttgaa gatgaacagg ctattcgtcg ctttctgcgc
     7921 acggcgctgg agggcgacgg gatgcgcgtc tatgaggccg aaacgctgca acgcggcttg
     7981 ctggaagcgg ctacccgtaa gccagatttg attattctcg atctcggcct gcccgatggt
     8041 gatgggattg agtttatccg cgacctgcgc cagtggagcg cggtgccggt gattgtgctt
     8101 tccgcacgca gcgaagagag cgacaaaatc gccgcgctgg atgccggagc ggatgattat
     8161 ctgagtaagc cgtttggcat tggcgaattg caggcccgtc tgcgcgtcgc tttacgccgc
     8221 cactccgcca ccgccgcgcc cgatccgctg gtgaaatttt ccgatgttac cgtcgattta
     8281 gccgcccgcg tgattcaccg gggtgaggaa gaggtgcatc tcacgccgat tgagttccgc
     8341 ctgctggcgg tgctgctgaa taatgccggg aaagtgctca cccagcgcca gttactcaat
     8401 caggtgtggg ggccaaacgc ggtcgaacac agtcactatt tgcgtattta tatgggacat
     8461 ctgcgacaaa aactggaaca ggatcccgct cgcccccgcc atttcattac tgaaaccggt
     8521 attgggtatc ggtttatgcc atgaatgttt attcaataaa tgcgcttaaa taattattaa
     8581 tttattatta aacctgccaa aaatattatt atttggcagg tttaatttct taacgcaaat
     8641 ataaaacaca aaattacaac atttaaataa caaaacagca tctaatatgg cgctattcat
     8701 aattaatgat ttattatttg gggtatgatc gttttttgtt gatcttcttc acagattata
     8761 gccatttcat ggatagaata actctacctt caactgacac agcaagaggt aaaggtaaat
     8821 ggaaaataat aaccgcttaa tgcctcatat aaggcggaca acccatatca tgaagtttgc
     8881 ccatcgtaat agttttgact ttcatttctt taatgcccgc tagtcttctg actaaagggc
     8941 accccaacgt acaggtctcc ctgactttaa gcattacagg ttaatacctg tattcctcgg
     9001 tgctcatata ctgctaaccc ttttaaactc taaataattc gagtcgcagc acttgcaact
     9061 tgaggtatga cgagtatagc cagttaccgg gctggtctgg gttattgcat ctgcaaaaag
     9121 caaactactg atttatttat cagcggtgga gctttgcttt ttttccggcg tgatcgattt
     9181 ctcctttgag aaattgagga cctgctatta cctgaaataa agagatgaac aaaatgtcag
     9241 aattaaaaat tgccgttagt cgttcttgcc cggattgttt ttccactcat cgtgcatgcg
     9301 tgaatataga cgaaagtaat tatattgacg ttgccgccat tattttatca gtcagtgatg
     9361 ttgaacgtgg aaaactcgat gaaatagacg ctactggcta tgacattcct gtttttattg
     9421 caacggaaaa tgaagaacgt gtacccgcag aatatctgcc acgtatttct ggtgtcttcg
     9481 aacattgcga atcacgtaaa gagttttatg gacgtcagtt agaaaccgct gccagccatt
     9541 atgaaacgca actgcgcccg cccttcttcc gcgcactggt cgattatgtt aatcagggca
     9601 atagtgcttt tgactgccct ggtcatcagg ggggtgagtt tttccgtcgc catccggcag
     9661 gcaatcagtt cgtggaatac tttggcgaaa tgctcttccg ctctgacttg tgtaatgctg
     9721 acgtagcaat gggcgatctg ctgattcatg aaggtgcgcc atgtattgca caacagcatg
     9781 cggctaaagt ctttaatgcc gataaaacct atttcgtttt aaacggtact tcatcttcta
     9841 ataaagtggt cttaaacgca ttgctgacgc cgggcgatct ggtgttgttt gaccgcaata
     9901 accataaatc taaccaccac ggtgcgttgc tacaggctgg cgcgacaccg gtttatctgg
     9961 aaaccgcacg taacccgtat ggttttatcg gtggtattga cgctcactgt tttgaagaaa
    10021 gttacctgcg tgagctgatt acagaagttg caccgcagcg ggcaaaagag gctcgtcctt
    10081 tccgcctcgc cgttattcag ctaggcactt acgacggtac gatttataac gcccgtcagg
    10141 tggtggataa aattggtcat ctgtgtgact acattctgtt tgactccgca tgggttggct
    10201 atgagcaatt tatcccgatg atggctgact gttcgccgct gttgctggat cttaatgaga
    10261 acgatccggg tattctggtt acgcaatccg tacataaaca gcaggctggt ttttctcaaa
    10321 cttcacaaat ccataaaaaa gacagccaca tcaaagggca acagcgttat gtaccgcaca
    10381 aacgcatgaa caacgccttt atgatgcacg cctccaccag cccgttctat ccgctgtttg
    10441 ccgcactgga tatcaacgcc aaaatgcatg aaggtgtcag cggtcgtaat atgtggatgg
    10501 attgtgtggt aaatggcatt aatgcccgca aactgatcct cgataactgt cagcatattc
    10561 gtccgttcgt acctgaactg gtggatggta aaccctggca gtcgtatgaa acagcgcaaa
    10621 ttgcggttga tctgcgcttc ttccagtttg taccagggga acactggcat tcttttgaag
    10681 gctatgcaga gaatcaatac tttgtcgatc cttgcaaact gttgctgaca accccaggta
    10741 ttgatgcacg taacggcgaa tatgaagcgt tcggtgtacc cgcgacgatt cttgctaact
    10801 tcctgcgcga aaatggcgta gtgccggaaa aatgcgatct taactccatc ctcttcctgc
    10861 tgactccggc agaagatatg gccaagcttc agcaacttgt tgccctgctg gtacgcttcg
    10921 aaaaactgct tgagtccgac gcgccattag cagaagtgct gccttccatc tacaaacagc
    10981 atgaagagcg ctacgccggt tataccctgc gtcagttgtg tcaagaaatg catgatttgt
    11041 atgcccgtca caacgtgaaa caactgcaaa aagagatgtt ccataaggaa cacttcccac
    11101 gcgtaagcat gaatccgcaa gaagccaact acgcctattt acgcggtgaa gtggagctgg
    11161 ttcgtctgcc ggatgcagaa ggccgtatcg ctgccgaagg tgcgcttcct tatcctccgg
    11221 gtgtgctgtg cgttgttccg ggtgaaatct ggggtggtgc tgttctgcgt tacttcagcg
    11281 ctctggaaga agggatcaac ctgttgccag gttttgcacc ggagcttcag ggtgtctata
    11341 tcgaagaaca tgatggtcgt aagcaagttt ggtgctatgt catcaagcct cgtgatgcgc
    11401 aaagtaccct gttgaaaggg gaaaaattat gagtcaggct aaatcgaaca aaatgggcgt
    11461 cgttcagtta accatactga cgatggtcaa catgatgggc tccggtatca tcatgctgcc
    11521 gacaaagctt gccgaagttg ggacaatttc aattatctcc tggctggtga cagccgtagg
    11581 ctcaatggca ctggcatggg cattcgcaaa atgcggtatg ttcagccgta aatcaggtgg
    11641 tatgggcggt tatgccgagt atgctttcgg taagtccggc aattttatgg cgaactacac
    11701 ctacggtgtc tcgctgctga ttgctaacgt cgcgattgct atttcggcgg ttggttacgg
    11761 caccgaactg ctcggcgcga gtttgtcgcc agtgcagatt ggtcttgcga ccatcggagt
    11821 gctgtggatt tgtaccgtgg ctaactttgg tggtgcgcgc attaccgggc aaatcagtag
    11881 cattactgtg tggggggtca ttattccggt ggtaggcctg tgcattatcg gctggttctg
    11941 gtttagcccg acgctttacg ttgattcctg gaatccgcat catgcaccgt tcttcagtgc
    12001 agtaggttct tccatcgcta tgacgctgtg ggcttttctc ggtctggagt ctgcgtgtgc
    12061 gaatactgat gtagtggaaa acccggaacg taatgtgcca atcgcggtac tcggcggtac
    12121 gttaggtgcg gcggtgattt atatcgtctc caccaacgtg attgcaggga ttgtgcctaa
    12181 tatggagctg gcaaattcaa cggcaccatt tggcctggcc ttcgcacaga tgttcacacc
    12241 agaagtgggt aaagtcatta tggcgctgat ggtgatgtcc tgctgcggtt cgctgcttgg
    12301 ctggcagttc accattgccc aggtgtttaa atcttcatct gatgaaggct acttccctaa
    12361 aattttctcc cgtgtaacca aagtggatgc accggtgcag ggaatgttga ccattgtgat
    12421 tatccagagc gggttggcgc tgatgacgat tagcccgtcg ctaaacagtc agttcaacgt
    12481 gctggttaac ctggccgtgg tgaccaatat cattccgtat attctgtcaa tggcggcatt
    12541 agtcattatt cagaaggtcg ctaatgtgcc cccctcaaaa gcgaaagttg caaactttgt
    12601 tgcttttgtt ggcgcgatgt atagctttta tgcgctgtac tcatccgggg aagaagccat
    12661 gctgtacggt tccatcgtga ccttccttgg ttggacactg tatggtctgg tatcaccacg
    12721 ctttgaactg aaaaataaac acggttaaaa aagcaaagta aaaaagggcg atcttgcgac
    12781 cgcccttttt ttattaaatg tgtttacgcg tttttcagaa cttcgctaac aatctcaacc
    12841 gcttctttct caatctgctt gcgatgttct tcaccgagga agctttcgca gtagatctta
    12901 tatgcgtctt ccgtgcctga cggacgcgcg gcgaaccagc cgttgtcggt catcactttc
    12961 agaccgccga tagaagcacc attgcccggt gccgccgtca ggcgcgcggt gatcgggtcg
    13021 cctgcaaggg tgctggcgct caccatttcc ggagacagct tagacagcgc cgctttttgt
    13081 gcggaagtcg cggctgcctg caaacggttg tagctcggcg caccaaagcg ttttgccagt
    13141 tcgttgtagt gttcctgcgg gttcttaccg gtgacagcgg tgatttccgc cgccagcaga
    13201 cacataatga tgccgtcttt gtcggtggac cacggcgtgc cgtcgaaacg caggaaggaa
    13261 gcccctgcgc tctcttcgcc gccaaagccg aagctgccgt cgaacagacc atcaacaaac
    13321 catttgaaac ctaccgggac ttctaccagc ttacggccca agtcattgac cacacggtcg
    13381 atcatcgcag aagaaaccag cgttttaccg acagcaacat ctttgcccca ctgcggacga
    13441 tgctggaaca ggtagttgat cgccaccgcc aggtagtggt tcggattcat caaacctgcc
    13501 ggagtgacga taccgtgacg gtcataatcc gggtcgttag caaacgccag atcgaactta
    13561 tcacgcagtg ccagcaggcc cgccatcgca cactcggagg agcagtccat acggatcgcg
    13621 ccgtctttat caaggtgcat aaagcggaag gtttgatcga cctgatcgtt aacgatggtc
    13681 aggttgaggt tgtaatactc gccaatacgc ttccagtatt cgataccgga accgcccagc
    13741 ggatcaacgc ccagcgtcag gcccgctttc tgaattgccg ccatatcaac gatatcggcc
    13801 agaccttcca caaacggctg caccagatcc tgctctttta catgaccgga tgccatcgct
    13861 tcgtcgaggg agatacgctt cacgcctttc aggccatcgg ccatcagtgc gttggccctg
    13921 tcttccacca ctttggtgac gttggtatca gccgggccac catttggcgg gttgtacttg
    13981 ataccaccat cttccggcgg gttatgggac ggtgtaatca cgataccgtc tgccagcggg
    14041 ccaccttttt tattgtgaac caggatggca ttggaaatgg caggtgtcgg agtgaagcca
    14101 ttgttttcct gcacaatgac atcaacgccg ttcgctgcca gcacttccag tacggaaatg
    14161 aacgcaggct cggacagggc gtgagtatct ttacccacat agcaagggcc agtgatgccg
    14221 tttttcgcac gttcttcagc aattgcctga gcgatcgcca gaatgtgcgg ctcgttaaag
    14281 ctgtggcgcg ctgcactgcc acggtgaccg gaagtaccga atttcaccgc gtgctccgca
    14341 ttccctgctt ctggtttcag tacataatat tgcgccgtca gttgggcgac gttaatcaaa
    14401 tcactctgtt gtgcaggttg gcctgcacga ttgtggattg ccattgcttt gtcctttgtc
    14461 tgcaacgttt tagatagttc cgcaaacctt ctcaatcaat tccgccggga attgcatcga
    14521 ctgcatgatg tgttcgatca tgctgcattt acggccggtg ttggtgttgg tgatcaccca
    14581 atacggcgtg cctggcacat gtttcggctt ggtctgatta ccatttttca gcagcgtttg
    14641 ttcatctgcc gcaaagtaaa cgcgtgtacg accgtgcaac gattccgttg cttcggcaaa
    14701 cgcctgggcg tcaagagaat atagtgtaga caacagcagc ataaagcgat tgaccgctcg
    14761 cttttgctct gcgtattcat ccgaaagcag aagttcacgc attgcgcgaa ccttgtcttt
    14821 aatcgttttg accggcttcg cttcgacgat agcaggtgac gcaacgcgaa cctctttcgt
    14881 caccggagca gcaggctgtg atgcggcgga aaatttcaac atacgccgta aaatgtcgga
    14941 tgcgctctcg ccgatatgct tagtgtggct ggcaatatag ctgtagagtt catcatcaac
    15001 ttcaatcgtt ttcatcttaa tccagtgcag tgtcttagct gaatacaagt cgccaagagt
    15061 atatgaataa atcccggcag cggatagcgt caagcctggc ttgcagaaaa actcggaata
    15121 aaccttattt gttgcagccc tgtggcacaa tggtcaacat aataccctaa cccgacaacg
    15181 cgaaaaaaag aactttgcca tgaaattgaa tatccgcgcg caaactgcac aaaaccagca
    15241 caataattct cccatcgttc ttgtccatgg tctgtttggt agcctcgaca accttggcgt
    15301 actggctcgc gatctggtaa acgatcacaa tatcatccag gttgatatgc gtaaccacgg
    15361 tctttcaccg agagatccgg taatgaatta cccggcgatg gcgcaggatc ttgttgatac
    15421 tttggatgca cagcagatcg acaaagcaac atttatcggt cactccatgg gcggtaaagc
    15481 ggtaatggca cttactgcac tggcccccga tcgcatcgat aaactggtgg cgatcgatat
    15541 cgcaccggtc gactatcacg tacgccgtca tgatgagatt ttcgcggcta tcaacgcggt
    15601 cagtgaatcg gacgcacaaa ctcgccagca agcagcagca ataatgcgcc agcatcttaa
    15661 tgaagaaggg gtgattcagt ttctgttgaa atcttttgtt gacggggagt ggcgctttaa
    15721 cgtgccggta ttgtgggatc agtatccgca tattgtgggt tgggagaaaa tcccggcatg
    15781 ggatcaccct gccctgttta tccctggcgg caattctccg tatgttagcg agcagtaccg
    15841 tgatgattta ctggcacaat ttccacaggc acgggcgcat gtgattgcag gcgcgggtca
    15901 ctgggtccat gctgaaaaac cggatgcggt attacgcgct atccgtcgct atctcaatga
    15961 ttaacgcaac tgattaaaaa cccagcgtcc gcgtcgcttt ccgcgcgtgg gcgttggcgt
    16021 gactcatcgc gctgatgtat tatggcgcgc tatccatccg ggcagaggcc tggctgattg
    16081 tttcccccga agtcaccaag atcatggcca aagaacaaac ggaccgtacg acattagatc
    16141 tgttcgcgca cgagcgtcga ccgggacgac cgaaaactaa tccgctttcg cgcgatgaac
    16201 agctgcgtat taataaacgc aaccagctaa aacgcgacaa agtacgtggc cttaagcgtg
    16261 tcgaactgaa gctgaacgcg gaagctgtcg aggcgctgaa cgagctggcg gagtcgcgca
    16321 atatgagccg tagcgaactg atcgaagaga tgttaatgca gcaactggcg gcgctgcgta
    16381 gtcagggcat cgtttaaatt tccactttca tgtagcacag tgtgcagtcc tgctcgtttg
    16441 ctgattccgc aagactgcct gttctgctat gattgccttt atccgtgggc aattttccac
    16501 ccccatttca ataagtttca agaggttatt tcactcatgg ctatcactgg catctttttc
    16561 ggcagcgaca ccggtaatac cgaaaatatc gcaaaaatga ttcaaaaaca gcttggtaaa
    16621 gacgttgccg atgtccatga cattgcaaaa agcagcaaag aagatctgga agcttatgac
    16681 attctgctgc tgggcatccc aacctggtat tacggcgaag cgcagtgtga ctgggatgac
    16741 ttcttcccga ctctcgaaga gattgatttc aacggcaaac tggttgcgct gtttggttgt
    16801 ggtgaccagg aagattacgc cgaatatttc tgcgacgcat tgggcaccat ccgcgacatc
    16861 attgaaccgc gcggtgcaac catcgttggt cactggccaa ctgcgggcta tcatttcgaa
    16921 gcatcaaaag gtctggcaga tgacgaccac tttgtcggtc tggctatcga cgaagaccgt
    16981 cagccggaac tgaccgctga acgtgtagaa aaatgggtta aacagatttc tgaagagttg
    17041 catctcgacg aaattcttaa tgcctgatgt gatgcggcgt agactcatgt ctacgccgta
    17101 ttaatagata atgccaatca aaataattgc tacaaatttg taacttttgc tgttgtacct
    17161 gtacaatgtc ccggtgttca agtggccttg ccgttgtaaa tgtaagctgt gccacgtttt
    17221 tattaacaat atttgccagg gacttgtggt tttcatttag gcgtggcaat tctataatga
    17281 tacgcattat ctcaagagca aattctgtca cttcttctaa tgaagtgaac cgcttagtaa
    17341 caggacagat tccgcatgac tgataacaat accgccctaa agaaagctgg cctgaaagta
    17401 acgcttccac gtttaaaaat cctggaagtt cttcaggagc cggacaacca tcacgtcagt
    17461 gcggaagatt tatacaaacg tctgatcgat atgggtgaag aaattggtct ggctacggta
    17521 tatcgcgtac tgaaccagtt tgacgacgct ggtatcgtca cccgccacaa ttttgaaggc
    17581 ggtaaatccg tatttgaact gacacagcaa catcaccacg atcacctgat ttgcctcgac
    17641 tgcggcaagg ttatcgaatt tagtgatgat tccatcgaag cgcgtcagcg tgaaattgcc
    17701 gcaaaacatg gcattcgcct gactaaccac agtctctatc tttacggtca ctgtgccgaa
    17761 ggcgattgcc gcgaagatga gcacgcgcac gaaggcaaat aagccagcct gaacgagaaa
    17821 agccaacctg cgggttggct tttttataca agggaaataa gaaaagcgag agttacagct
    17881 ctcacttatt tgttattact gcgtatttcc tgccagacct tatcacaatc ggctttcacc
    17941 gcctgatcat ttccggtttg cgttgcacgc agacactgct gataatccag tacgcgtaca
    18001 ctttcctggt cggcaaactg ctgatgttgt ttctctttct tcagcacgtt taacacgctc
    18061 tggcaggctt caattttttc tggcgaacct tgtgcggtgt tgatacaggc gctgtatgcc
    18121 tctttcagac gactgtcttc ctgtggcgcg gttgattgcg cacaagctac cagccccgat
    18181 gccattatgg cgatgagaat cagttttttc atagcaaatt cctcaaacgt gcggcaggca
    18241 caaaacctgc cgcgtcgggc atcagaagat ggtgaatggt gcgattacca taaatttcac
    18301 gtcacgctca tcctggaaga tgttaccgta accgccgccc cagcttggga tgtcagagtg
    18361 gttgtcgtat tcggtgaagt gcagtttgaa catcgtgcct ttggcgcgac cgtcctgaat
    18421 ggtgtagacc gcatccaggc tgtaggcaga ctcttcaata gtccggtttt tgtcgtagta
    18481 cgcattcgga ttgctctgcc aggtcgcagg tttagcatcc catgcgtaaa cgtaggaagc
    18541 gccgatggcg aagcctggaa gattccagtt tttcaggtca tacatcgcac cgaagaagac
    18601 cgctttttcg ccgttggcgt tgaagtcgga acggttatcc caccagatat ccaggcggcc
    18661 gttggaggaa gcgtaggttg gagtcatacg ttgcaggaag tatccctgct gaccgtcagc
    18721 cttaacccag gtgccttcga ggcgcaaatc cactacgtca gccgcccggt aaccaaaggt
    18781 caacgcttgc agccaggcgg tgctgtcata aaggtcgttg acgctgcgat cgtcaacttt
    18841 atcgcgcgta ccatagaact ggtagctggt ggttaacggg ctaccggcga tatcaaattt
    18901 gtagctggct ttggcaaaat attgatcgat atacccttcc gcctgaccaa atgccgcttc
    18961 cagtacgaag ttatttttga agtcgtattt cgccccaagg gagtgcagat aatcaacttt
    19021 ggtggtttta tcgttctgat aaaactcatc catttccaga tgccacggcg ctttgtattc
    19081 gttggtccac atgtaggaga aactcaacgc accagcatcg ccgtaatcaa aattcgcccc
    19141 ggcttccgcc ccctgataag tacctggcat aaagctccag tgcggcgcta acagcgtttg
    19201 accagttggc tgaatgtaac ctgccctcgc ccaaaccgga ccgtatttaa atttggccgc
    19261 agctttatac aggcttatac cgcttttgtc gccggaccag tcttcgtcat aggctttatt
    19321 actttttgaa aacgcgattt cgttcgggtg ggagctgtcg ccgttttccg ccatttcaat
    19381 cgccgtaaac gcagcaatat caaggccgaa catatcagca gcataaccgg actgaaaatc
    19441 gaggttggca ttccaggtgg agtgagaaag gttggttttg tatttgtcgc cgtcggtaac
    19501 atctttacgg tcgcgttcac gctgccagta atatataccg ccggttaagg ttgaatcgtc
    19561 gatgaatcct tcggcgcgag cctccggcat tgccataaag cccgacattg ctgtaacacc
    19621 ggcgatagcc agcgccagcg tactacgttt gccactaaac gtacgcatgg gttaatcctc
    19681 tttgacgtat aaattgctgc accaaaggtg aacagcgaaa aaataaaaat taaaaaagta
    19741 gcgttgagaa aagtctcgct gactacagga acagactatt tttcgcgacg cgaattatca
    19801 atctctttct gtaaccagaa tgtaagaaca tgagcaaccg cacatatttt gatgattttt
    19861 gttacaaggc ttaagtttgc ggttgaaagg cttgcagaag aataaaagtg tgaatttcca
    19921 gc
//
LOCUS       DAZFQD010000098        19404 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.098, whole genome shotgun sequence.
ACCESSION   DAZFQD010000098 DAZFQD010000000
VERSION     DAZFQD010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19404
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.098"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            178..267
                     /locus_tag="ACHKTW_003454"
                     /pseudo
     CDS             178..267
                     /locus_tag="ACHKTW_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308358.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            741..842
                     /gene="ykgR"
                     /locus_tag="ACHKTW_003455"
     CDS             741..842
                     /gene="ykgR"
                     /locus_tag="ACHKTW_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YkgR"
                     /protein_id="HFO6464410.1"
                     /translation="MKENKVQQISHKLINIVVFVAIVEYAYLFLHFY"
     gene            1205..1468
                     /gene="ykgM"
                     /locus_tag="ACHKTW_003456"
     CDS             1205..1468
                     /gene="ykgM"
                     /locus_tag="ACHKTW_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFO6464411.1"
                     /translation="MKPNIHPEYRTVVFHDTSVDEYFKIGSTIKTDREIELDGVTYPY
                     VTIDVSSKSHPFYTGKLRTVASEGNVARFTQRFGRFVSTKKGA"
     gene            1468..1608
                     /gene="ykgO"
                     /locus_tag="ACHKTW_003457"
     CDS             1468..1608
                     /gene="ykgO"
                     /locus_tag="ACHKTW_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HFO6464412.1"
                     /translation="MKVLNSLRTAKERHQDCQIVKRKGRLYVICKSNPRFKAVQGRKK
                     KR"
     gene            complement(1643..1876)
                     /gene="ykgL"
                     /locus_tag="ACHKTW_003458"
     CDS             complement(1643..1876)
                     /gene="ykgL"
                     /locus_tag="ACHKTW_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YkgL"
                     /protein_id="HFO6464413.1"
                     /translation="MKTQAAPQREETEWRVQSKRGLMPAYRGEAGQQVNIKIMEYSER
                     NVRQLASNEQEEYIPRKINVGVINTPTLIRSDY"
     gene            2694..3236
                     /gene="ecpR"
                     /locus_tag="ACHKTW_003459"
                     /gene_synonym="matA"
     CDS             2694..3236
                     /gene="ecpR"
                     /locus_tag="ACHKTW_003459"
                     /gene_synonym="matA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECP biosynthesis operon DNA-binding
                     transcriptional regulator EcpR"
                     /protein_id="HFO6464414.1"
                     /translation="MECQNRSDKYIWSPHDAYFYKGLSELIVDIDRLIYLSLEKIRKD
                     FVFINLNTDSLSEFINRDNEWLSAVKGKQVVLIAARKSEALANYWYYNSNIRGVVYAG
                     LSRDIRKELAYVINGRFLRKDIKKDKITDREMEIIRMTAQGMQPKSIARIENCSVKTV
                     YTHRRNAEAKLYSKIYKLVQ"
     gene            3311..3898
                     /gene="ecpA"
                     /locus_tag="ACHKTW_003460"
                     /gene_synonym="matB"
     CDS             3311..3898
                     /gene="ecpA"
                     /locus_tag="ACHKTW_003460"
                     /gene_synonym="matB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="common pilus major fimbrillin subunit EcpA"
                     /protein_id="HFO6464415.1"
                     /translation="MKKKVLAIALVTVFTGMGVAQAADVTAQAVATWSATAKKDTTSK
                     LVVTPLGSLAFQYAEGIKGFNSQKGLFDVAIEGDSTATAFKLTSRLITNTLTQLDTSG
                     STLNVGVDYNGAAVEKTGDTVMIDTANGVLGGNLSPLANGYNASNRTTAQDGFTFTII
                     SGTTNGTTAVTDYSTLPEGIWSGDVSVQFDATWTS"
     gene            3956..4624
                     /gene="ecpB"
                     /locus_tag="ACHKTW_003461"
                     /gene_synonym="yagY"
     CDS             3956..4624
                     /gene="ecpB"
                     /locus_tag="ACHKTW_003461"
                     /gene_synonym="yagY"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpB"
                     /protein_id="HFO6464416.1"
                     /translation="MKKHLLPLALLFSGIPPAQALDVGDISSFMNSDSSTLSKTIKNS
                     TDSGRLINIRLERLSSPLDDGQVISMDKPDELLLTPASLLLPAQASEVIRFFYKGPAD
                     EKERYYRIVWFDQALSDAQRDNANRSAVATASARIGTILVVAPRQANYHFQYANGSLT
                     NTGNATLRILAYGPCLKAANGKECKENYYLMPGKSRRFTRVDTADNKGRVALWQGDKF
                     IPVK"
     gene            4650..7175
                     /gene="ecpC"
                     /locus_tag="ACHKTW_003462"
                     /gene_synonym="yagX"
     CDS             4650..7175
                     /gene="ecpC"
                     /locus_tag="ACHKTW_003462"
                     /gene_synonym="yagX"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher EcpC"
                     /protein_id="HFO6464417.1"
                     /translation="MPLRRFSPGLKAQFAFGMVFLFVQPDASAADISAQQIGGVIIPQ
                     AFSQALQDGMSVPLYIHLAGSQGRQDDQRIGSAFIWLDDGQLRIRKIQLEESEDNASV
                     SEQTRQQLMALANAPFNEALTIPLTDNAQLDLSLRQLLLQLVVKREALGTVLRSRSED
                     IGQSSVNTLSSNLSYNLGVYNNQLRNGGSNTSSYLSLNNVTALREHHVVLDGSLYGIG
                     SGQQDSELYKAMYERDFAGHRFAGGMLDTWNLQSLGPMTAISAGKIYGLSWGNQASST
                     IFDSSQSATPVIAFLPAAGEVHLTRDGRLLSVQNFTMGNHEVDTRGLPYGIYDVEVEV
                     IVNGRVISKRPQRVNKLFSRGRGVGAPLAWQVWGGSFHMDRWSENGKKTRPAKESWLA
                     GASTSGSLSTLSWAATGYGYDNQAVGETRLTLPLGGAINVNLQNMLASDSSWSSIGSI
                     SATLPGGFSSLWVNQEKTRIGNQLRRSDADNRAIGGTLNLNSLWSKLGTFSISYNDDR
                     RYNSHYYTADYYQNVYSGTFGSLGLRAGIQRYNNGDSNANTGKYIALDLSLPLGNWFS
                     AGMTHQNGYTMANLSARKQFDEGTIRTVGANLSRAISGDTGDDKTLSGGAYAQFDARY
                     ASGTLNVNSAADGYINTNLTANGSVGWQGKNIAASGRTDGNAGVIFNTGLEDDGQISA
                     KINGRIFPLNGKRNYLPLSPYGRYEVELQNSKNSLDSYDIVSGRKSHLTLYPGNVAVI
                     EPEVKQMVTVSGRIRAEDGTLLANARINNHIGRTRTDENGEFVMDVDKKYPTIDFRYS
                     GNKTCEVALELNQARGAVWVGDVVCSGLSSWAAVTQTGEENES"
     gene            7165..8808
                     /gene="ecpD"
                     /locus_tag="ACHKTW_003463"
     CDS             7165..8808
                     /gene="ecpD"
                     /locus_tag="ACHKTW_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial adhesin EcpD"
                     /protein_id="HFO6464418.1"
                     /translation="MRVNLLITMIIFALIWPVTELRAAVSKTTWADAPAREFVFVENN
                     SDDNFFVTPGGALDPRLTGANRWTGLKYTGSGTIYQQSLGYIDNGYNTGLYTNWKFDM
                     WLENSPVSSPLTGLRCINWYAGCNMTTSLILPQTTDASGFYGATVTSGGAKWMHGMLS
                     DAFYQYLQQMPVGSSFTMTINACQTSVNYDASSGARCKDQATGNWYVRNVTHTKAANL
                     RLINTHSLAEVFINSDGVPTLGEGNADCRTQTIGSLSGLSCKMVNYTLQTNGLSNTSI
                     HIFPAIANSSLASAVGAYDMQFSLNGSSWKPVSNTAYYYTFNEMKSADSIYVFFSSNF
                     FKQMVNLGISDINTKDLFNFRFQNTTSPESGWYEFSTSNTLIIKPRDFSISIISDEYT
                     QTPSREGYVGSGESALDFGYIVTTSGKTAADEVLIKVTGPAQVIGGRSYCVFSSDDGK
                     AKVPFPATLSFITRNGATKTYDAGCDDSWRDMTDALWLTTPWTDISGEVGQMDKTTVK
                     FSIPMDNAISLRTVDDNGWFGEVSASGEIHVQATWRNIN"
     gene            8777..9487
                     /gene="ecpE"
                     /locus_tag="ACHKTW_003464"
     CDS             8777..9487
                     /gene="ecpE"
                     /locus_tag="ACHKTW_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpE"
                     /protein_id="HFO6464419.1"
                     /translation="MFRRRGVTLTKALLTAVCMLAAPLTQAISVGNLTFSLPSETDFV
                     SKRVVNNNKSARIYRIAISAIDSPGSSELRTRPVDGELLFAPRQLALQAGESEYFKFY
                     YHGPRDNRERYYRVSFREVPTRNQTRRSPTGGEVSTEPVVVMDTILVVRPRQVQFKWS
                     FDKVTGTVSNTGNTWFKLLIKPGCDSTEEEGDAWYLRPGDVVHQPELRQPGNHYLVYN
                     DKFIKISDSCPAKPPSAD"
     gene            9800..10129
                     /gene="ykgJ"
                     /locus_tag="ACHKTW_003465"
     CDS             9800..10129
                     /gene="ykgJ"
                     /locus_tag="ACHKTW_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HFO6464420.1"
                     /translation="MSNLNPCMTCGACCAFFRVSFYWAEADDAGGTIPARLTEQISPF
                     HRCMSGTNQKNPRCIALAGTPGKNACCTIYKNRSSTCREFAMSGENGVVNEACNRARA
                     KYGLTPL"
     gene            complement(10377..10991)
                     /gene="yagU"
                     /locus_tag="ACHKTW_003466"
     CDS             complement(10377..10991)
                     /gene="yagU"
                     /locus_tag="ACHKTW_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YagU family protein"
                     /protein_id="HFO6464421.1"
                     /translation="MNIFEQTPPNRRRYGLAAFIGLIAGVVSAFVKWGAEVPLPPRSP
                     VDMFNAACGPESLIRAAGQIDCSRNFLNPPYIFLRDWLGLTDPNAAVYTFAGHVFNWV
                     GVTHIIFSIVFAVGYCVVAEVFPKIKLWQGLLAGALAQLFVHMISFPLMGLTPPLFDL
                     PWYENVSEIFGHLVWFWSIEIIRRDLRNRITHEPDPEIPLGSNR"
     gene            11409..12098
                     /gene="paoA"
                     /locus_tag="ACHKTW_003467"
     CDS             11409..12098
                     /gene="paoA"
                     /locus_tag="ACHKTW_003467"
                     /EC_number="1.2.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0047770 - carboxylate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase iron-sulfur subunit PaoA"
                     /protein_id="HFO6464422.1"
                     /translation="MSNQGEYPEDNRVGKHEPHDLSLTRRDLIKVSAATAATAVFYPH
                     STLAASVPAATPAPEIMPLTLKVNGKTEQLEVDTRTTLLDALRENLHLIGTKKGCDHG
                     QCGACTVLVNGRRLNACLTLAVMHQGAEITTIEGLGSPDNLHPMQAAFIKHDGFQCGY
                     CTSGQICSSVAALKEIQDGIPSHVTVDLVSAPETTADEIRERMSGNICRCGAYANILA
                     AIEDAAGEIKS"
     gene            12095..13051
                     /gene="paoB"
                     /locus_tag="ACHKTW_003468"
     CDS             12095..13051
                     /gene="paoB"
                     /locus_tag="ACHKTW_003468"
                     /EC_number="1.2.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde oxidoreductase FAD-binding subunit
                     PaoB"
                     /protein_id="HFO6464423.1"
                     /translation="MKAFTYERVNTPAEAALSAQRVPGAKFIAGGTNLLDLMKLEIET
                     PTHLIDVNGLGLDKIEVTDAGGLRIGALVRNTDLAAHERVRRDYAVLSRALLAGASGQ
                     LRNQATTAGNLLQRTRCPYFYDTNQPCNKRLPGSGCAALEGFSRQHAVVGVSEACIAT
                     HPSDMAVAMRLLDAVVETITPEGKTRSITLADFYHPPGKTPHIETALLPGELIVAVTL
                     PPPLGGKHIYRKVRDRASYAFALVSVAAIIQPDGSGRVALGGVAHKPWRIEAADAQLS
                     QGAQAVYDALFASAHPTAENTFKLLLAKRTLASVLAEARAQA"
     gene            13048..15246
                     /gene="paoC"
                     /locus_tag="ACHKTW_003469"
     CDS             13048..15246
                     /gene="paoC"
                     /locus_tag="ACHKTW_003469"
                     /EC_number="1.2.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde oxidoreductase molybdenum-binding
                     subunit PaoC"
                     /protein_id="HFO6464424.1"
                     /translation="MKFDKPAGENPIDQLKVVGRPHDRIDGPLKTTGTARYAYEWHEE
                     APNAAYGYIVGSAIAKGRLTALDTDAAQKAPGVLAVITASNAGALGKGDKNTARLLGG
                     PTIEHYHQAIALVVAETFEQARAAASLVQAHYRRNKGAYSLADEKQAVNQPPEDTPDK
                     NVGDFDGAFTSAAVKIDATYTTPDQSHMAMEPHASMAVWDGNKLTLWTSNQMIDWCRT
                     DLAKTLKVPVENVRIISPYIGGGFGGKLFLRSDALLAALAARAVKRPVKVMLPRPSIP
                     NNTTHRPATLQHLRIGADQSGKITAISHESWSGNLPGGTPETAVQQSELLYAGANRHT
                     GLRLATLDLPEGNAMRAPGEAPGLMALEIAIDELAEKAGIDPVEFRILNDTQVDPADP
                     TRCFSRRQLIECLRTGADKFGWKQRNATPGQVRDGEWLVGHGVAAGFRNNLLEKSGAR
                     VHLEQNGTVTVETDMTDIGTGSYTILAQTAAEMLGVPLEQVAVHLGDSSFPVSAGSGG
                     QWGANTSTSGVYAACMKLREMIASAVGFDPEQSQFADGKITNGTRSATLHEATAGGRL
                     TAEESIEFGTLSKEYQQSTFAGHFVEVGVHSATGEVRVRRMLAVCAAGRILNPKTARS
                     QVIGAMTMGMGAALMEELAVDDRLGYFVNHDMAGYEVPVHADIPKQEVIFLDDTDPIS
                     SPMKAKGVGELGLCGVSAAIANAVYNATGIRVRDYPITLDKLLDKLPDVV"
     gene            15256..16212
                     /gene="paoD"
                     /locus_tag="ACHKTW_003470"
     CDS             15256..16212
                     /gene="paoD"
                     /locus_tag="ACHKTW_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor insertion chaperone PaoD"
                     /protein_id="HFO6464425.1"
                     /translation="MSYPLFDKDEHWHKPEQAFLTDDHRTILRFAVEALMSGKGAVLV
                     TLVEIHGGAARPLGAQMVVREDGRYCGFVSGGCVEAAAAFEALEMMGSGRDREIRYGE
                     GSPWFDIVLPCGGGITLTLHKLRSAQPLLAVLNRLEQRKPAGLRYDPQAQSLVCLPTQ
                     TRTGWNLNGFEVGFRPCVRLMIYGRSLEAQATASLAAATGYDSHIFDLFPASASAQID
                     TDTAVILLCHDLNRELPALQAAREAKPFYLGALGSHRTHTLRLQKLHELGWSREETAQ
                     IRAPVGIFPKARDAHTLALSVLAEIASVRLHHEEDSCLPPSS"
     gene            16263..16601
                     /locus_tag="ACHKTW_003471"
                     /pseudo
     CDS             16263..16601
                     /locus_tag="ACHKTW_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014831000.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
     gene            complement(16728..16803)
                     /locus_tag="ACHKTW_003472"
     tRNA            complement(16728..16803)
                     /locus_tag="ACHKTW_003472"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16768..16770),aa:Thr,seq:cgt)
     gene            complement(16918..18171)
                     /gene="proA"
                     /locus_tag="ACHKTW_003473"
     CDS             complement(16918..18171)
                     /gene="proA"
                     /locus_tag="ACHKTW_003473"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFO6464426.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLGEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGSVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESAEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALAQLQTGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            complement(18183..19286)
                     /gene="proB"
                     /locus_tag="ACHKTW_003474"
     CDS             complement(18183..19286)
                     /gene="proB"
                     /locus_tag="ACHKTW_003474"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFO6464427.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
BASE COUNT         4875 a         5115 c         4924 g         4490 t
ORIGIN      
        1 tatatttttt atggatattc attttccttc atcggactgt aaattgcgga gatttcgaac
       61 ttgcactacc catcgcaaca ctcaaacaga tttatgaatt tcgttcataa cccctgacaa
      121 atcctgccgt ctaatcgagc ttttcccttt acgtcatgct tctcatgaac gagaacacaa
      181 cttattcatg aattaaccat tccggataaa ctatggaatc aaattcaggc atcgaaagga
      241 actggctggt ttccgttaat agattaaata tctattactc tcccgagagt aaaactatca
      301 aatcaatgag taaccgatac agcctcacaa attctttcag ataattaacc cgtcactgcg
      361 gcacgttctc tcgtaaggcg tgcctgcagt gtaatccagt atgtatccgg catagttgac
      421 ggtttaatag aactcaattc ctctcccagg tcaccaacaa cgatatcttg tataacaccc
      481 caggagatac gacattatga tgagatgcga tatgctcctc agcgccaatc aacgcccgga
      541 ctgggccagt cttgagatca gactcattcc cggaactgac ttcaggcgac atatatcaat
      601 gacgttggtg caataaaaga tagccgatgt aatacatcta ttcaaatttg tgatacatgt
      661 caaaaaattg gttgaccaaa ctcgttattt atataagggc acttacgaag tgcgctcttt
      721 tttaaagcga ggaagtacca atgaaagaga ataaagtaca acaaatcagt cataaactga
      781 ttaatatcgt tgtttttgtc gcaattgtag aatacgccta tttatttctc catttctatt
      841 aataacggca ataaactgtt cacttcagtg atatttaaaa tatgcatcct ctcccttttt
      901 gtaagtaatt attatatccg tgggagagga atacacgctg tcaggtaatc aatcatactg
      961 cgataaatca tcggccagta aagtggagat aacctccatt gtcgaaaaat ccatactctc
     1021 agcaaaacca tcatcaatca ctcatccagg cgtttatggg agcgtcacca atggctgcta
     1081 acaatgccag agttccccgt tgcgaaaatt ccacatccac aaagagtcac agggattggg
     1141 tgttgaaatg atccggatga gcatgtatct ttatgattat gttataacat aacaggtaaa
     1201 aatgatgaag cccaatatcc atcctgagta tcgtactgtg gtgttccacg acaccagtgt
     1261 tgatgagtac tttaaaatcg gctcgactat caaaacagac cgtgagattg agctggatgg
     1321 cgtaacgtat ccgtacgtaa caattgatgt ctcttcgaaa tcgcacccgt tctatacagg
     1381 gaagctgaga acagtggcat cagaaggaaa tgttgcacgg ttcacccaac gttttggtcg
     1441 ttttgttagc acgaaaaagg gggcgtaatg aaagttctta actctctgcg taccgcaaaa
     1501 gaacgccatc aagactgtca gattgtgaag cgaaaaggac ggttgtatgt gatttgtaaa
     1561 tctaatccac gttttaaggc cgttcagggt cgtaagaaaa aacgttgatt caaaattcga
     1621 cgaattaacg atatttgtct gattaataat cagatcggat taatgttggt gtgtttataa
     1681 caccaacatt aattttcctg gggatatatt cttcctgttc atttgaggcc aactgcctga
     1741 cgtttctctc cgaatattcc attatcttaa tgttgacttg ttgaccagct tcgcccctgt
     1801 atgctggcat caaccctctt ttagactgaa cacgccactc agtctcctcc ctttgcggcg
     1861 cagcctgcgt tttcactcaa actgttaaga tgataaatgt ggtaaatctg ttggtactaa
     1921 cataaaaacg tttacgccac aggaacagtt tgatccaccg gtaagcccat cgccgacgtt
     1981 cgagtgccag ttagagtaac gcgcacagat aactgcactc agtgccctga caaagaggcc
     2041 atcgttcctg tgacagctgg cagccttcgt ttaacttcac ttaatctggc tcttgggggt
     2101 cttaccgaac agatgacgta catacgcccg ttcaattttc catcacttat tgtaatgaac
     2161 acctataacc attttgtgcg gcatgttaat ccattaaaat ttcttactga ttggcaaatc
     2221 atcttcaatg acagctcatt atagttttat attctatccc ttacccttaa aacttgtttt
     2281 tttactagtc catcacacag cgcattaaga ctattcctaa cacttcaggg caaagttcct
     2341 gaccaatata aaatgcaagt aataattgaa cgttatattg ccaataacct tatgaaacca
     2401 aatgtctttt tcttcttatc aaaaaagcaa tattttcagt ttttctaaat attgacttaa
     2461 ccattgaatt ccttttccgt tcacatattg acactcatcg ggaaaaaaac ataaatttaa
     2521 gcccaatcga aaataattaa acttaatctc gtttaacctt tattgatatg tactacgtat
     2581 cttatttact tccggtttac taaggaaact gaatgcacct gtaaaaatta caggtttgga
     2641 aagtagtgac atggcaaagt gattacagca gggactatga ggttaaaaac catatggaat
     2701 gtcaaaaccg ttctgataaa tacatctggt ctccccatga cgcctacttc tataaaggac
     2761 tatctgaact gattgtggat atcgacagat taatttatct atcgttggag aaaattagaa
     2821 aagatttcgt gtttatcaat ctcaatacgg attctttatc tgaatttata aaccgtgata
     2881 atgaatggtt atccgcggta aaggggaaac aggtcgtatt gattgcggcc agaaagtcag
     2941 aagccttagc aaattattgg tattacaaca gcaatattag gggcgtggta tacgcaggac
     3001 tgagtcgtga tattagaaaa gaactggcct atgtgattaa tggcaggttc ctgagaaaag
     3061 atattaagaa agataaaatc actgaccggg aaatggaaat tatccgcatg acggcccagg
     3121 gaatgcaacc taaatcgatt gccagaattg aaaattgtag tgtgaagaca gtgtataccc
     3181 atcggcgtaa tgctgaggcc aagctgtact caaaaatata taagttggtt cagtaaactc
     3241 caggcaagtt agttttaaaa aatgactcac tgggacatca cgtcctcaat tcaactcggg
     3301 aagaaatgca atgaaaaaaa aggttctggc aatagctctg gtaacggtgt ttaccggcat
     3361 gggtgtggcg caggctgctg acgtaacagc tcaggctgta gcgacctggt cggcaacagc
     3421 caaaaaagac accaccagta agctggttgt gacgccactc ggtagcctgg cgttccagta
     3481 tgccgaaggc attaaaggtt ttaactcaca gaaaggtcta tttgacgtgg ctatcgaggg
     3541 tgactcaacg gctaccgcct ttaaactgac ctcacgtctt atcaccaaca cattaaccca
     3601 gttggatacc tcaggttcca cactgaatgt gggcgtggat tataacggcg cggcagtcga
     3661 aaaaactggc gataccgtga tgatcgatac cgccaacggc gtactgggcg gcaaccttag
     3721 cccgctggct aacggttaca atgccagcaa tcgtaccacc gcacaggatg gtttcacctt
     3781 caccatcatc agcggcacca ccaatggtac caccgcagta accgattaca gcactctacc
     3841 ggaaggcatc tggagcggcg acgttagcgt acagttcgac gcgacctgga ccagttaatc
     3901 tctctgatgt accagcaggg gtagcccccc tgctttattc ctggacggaa ttgttatgaa
     3961 aaagcacctt ctgcctctcg ctctgctgtt ttccggaata cctccggccc aggcgctgga
     4021 tgtcggcgat atatcatcgt ttatgaacag tgacagcagc acgctgagca aaacgatcaa
     4081 aaacagtacc gacagtggtc gccttatcaa tatccgtctc gaacggctct cttcaccgct
     4141 tgacgacggg caggttatct caatggacaa gccggatgag ttgctactca ctcccgccag
     4201 cttgctgcta cccgcccaag ccagcgaagt gatccgcttc ttctataagg gacccgcaga
     4261 tgaaaaagag cgctactacc gcattgtctg gtttgatcag gccctcagtg atgcgcagcg
     4321 cgataatgcc aaccgcagcg ctgtggccac tgcttccgcc cgcatcggca ccattctggt
     4381 cgtcgcccct cgccaggcga actaccactt tcagtacgcc aacggctccc tgacaaatac
     4441 aggaaatgcg acgctgcgga tcctcgccta cggcccttgc ctgaaagccg ccaacggtaa
     4501 ggagtgtaaa gagaattact acctgatgcc gggcaagtcg cgtcgtttta cccgtgtgga
     4561 cactgcggat aacaaaggac gggttgcact ttggcagggt gataagttca ttcccgtgaa
     4621 atagatagct gtgcagatgg ataacgacaa tgcctttacg acggttctcc ccaggactga
     4681 aagcccagtt tgccttcggc atggtctttt tgttcgttca acccgatgcc agcgctgctg
     4741 acataagtgc gcagcaaata ggtggggtga ttattccgca ggccttcagt caggcgcttc
     4801 aggacggcat gagcgtcccg ctctatattc atctcgccgg tagccagggt cgccaggacg
     4861 atcagcgaat cggcagcgct tttatctggc tggatgatgg acagctacgc atccggaaaa
     4921 tacagctgga agagagtgaa gataacgcca gtgtcagcga acaaactcga cagcagctga
     4981 tggctctggc caacgccccg ttcaatgagg cccttaccat ccccctgact gacaacgcgc
     5041 agctggatct cagcttgcgc caactgctgc tgcagctggt ggtcaagcgc gaagcgctgg
     5101 gcaccgtact acgctcacgt agcgaagaca tcgggcagtc cagtgttaac accctcagca
     5161 gtaatctgag ctataacttg ggcgtctata acaaccagtt gcgtaacggt gggagcaaca
     5221 catccagcta tctgtcgctg aataacgtta ctgcgctgcg cgaacatcat gtggtgctcg
     5281 acggctcgct gtacgggatc ggtagcggtc aacaggacag tgaattatat aaagcgatgt
     5341 atgaacgcga ttttgccggt caccgatttg ccggtggaat gctcgacacc tggaacttgc
     5401 agtccttagg gccgatgacc gccatttcag cagggaagat ttacggcctt tcctggggaa
     5461 accaggccag ctccaccatc ttcgacagca gccagtcagc cacgccagtg atcgcctttt
     5521 taccggcggc gggcgaagta catctcaccc gtgatgggcg gctactaagc gttcagaact
     5581 tcactatggg caatcatgaa gtggataccc ggggtctacc gtacgggatt tacgatgtgg
     5641 aagttgaggt gatcgttaac ggtcgcgtga tcagcaaacg cccccagcgg gtcaataagc
     5701 tgtttagccg ggggcgcggc gtcggtgcac cactggcgtg gcaggtatgg ggcggtagct
     5761 ttcatatgga tcgctggtcg gaaaacggga aaaagacgcg accagctaaa gagagttggc
     5821 tagcaggtgc ctcgacctcc ggctcattga gtacgcttag ctgggcggca acgggatatg
     5881 gatacgataa tcaggcggtg ggtgaaaccc gtctgacgct gccgcttggg ggggcgatca
     5941 acgttaacct gcaaaatatg ctggccagtg acagctcatg gagcagcatc ggcagcatca
     6001 gcgccactct accgggaggc tttagttcgc tgtgggttaa ccaggaaaaa acccgcattg
     6061 gcaatcaatt gcgacgtagc gatgccgaca accgtgcaat cggcggcaca ctcaacctga
     6121 actcactgtg gtcgaagctg ggcacattca gcatcagcta caatgatgac cgccgttaca
     6181 acagccatta ttacacggca gattactatc aaaatgtcta cagcggtacc tttggttcgc
     6241 ttggcctgcg ggccggtatt cagcgctata acaacggcga cagcaacgcc aatacaggga
     6301 aatatatcgc tctcgatctc tcgctaccac tgggcaactg gtttagcgca gggatgaccc
     6361 atcaaaacgg ctacaccatg gcaaacctgt cagcacgcaa acagtttgat gaaggaacca
     6421 ttcgcactgt tggtgccaat ctgtcacgtg ccatctccgg cgataccggt gatgacaaaa
     6481 ccctcagcgg tggggcgtat gcacagttcg acgctcgtta cgccagcgga acgctgaacg
     6541 tcaatagcgc ggcggacggc tacatcaata ccaacttgac cgccaatggc agcgtcggct
     6601 ggcagggtaa aaacatcgct gccagcgggc ggactgatgg caacgctggg gtgatattca
     6661 acaccgggct ggaggacgac ggtcagatca gcgccaaaat caacgggcgg attttcccgc
     6721 ttaacggcaa gcgtaactat ctcccgctct ctccctatgg aagatatgag gtggagttac
     6781 agaacagcaa aaactcactc gacagctacg atatcgtcag cggccgcaaa agccatctga
     6841 ctctctatcc aggcaatgtc gctgtcattg agccagaggt gaagcagatg gttaccgtct
     6901 ccggtcgtat ccgtgcggaa gacggcacac tgctggctaa cgcacggatt aacaaccata
     6961 tcggccgaac ccgaaccgat gaaaacggcg agtttgtcat ggacgtggat aagaaatatc
     7021 ccactatcga ttttcgctac agtggcaata aaacctgcga agtggcactg gaactcaacc
     7081 aggcgcgcgg tgccgtctgg gtcggtgatg tggtctgcag cggcctctca tcgtgggcgg
     7141 cggtgacgca gacaggagaa gagaatgaga gttaacctac taataacgat gataattttc
     7201 gcgctaatct ggccagtaac tgagctcaga gcggcagtga gcaaaacaac ctgggcggat
     7261 gcaccggcac gcgagtttgt gtttgtcgaa aacaactcag acgacaactt tttcgtcact
     7321 cctggcgggg cgctggatcc gcgcctgacc ggtgccaacc gctggaccgg tttaaaatac
     7381 actggttcag gaaccatcta tcagcaaagc ctcggctaca ttgataacgg ttacaacacc
     7441 ggcctttata ccaactggaa gtttgatatg tggctggaaa attcaccagt ttcatctcct
     7501 ttaactggct tgcgctgcat caactggtac gctgggtgta atatgaccac cagtcttatc
     7561 ctgccgcaaa ccaccgacgc cagtggattt tatggcgcga cggtgaccag cggcggcgcg
     7621 aagtggatgc acggcatgtt gtcagacgcg ttttaccagt atctgcaaca aatgcccgtc
     7681 ggcagcagct ttacaatgac catcaatgcc tgccagacct ctgtgaacta tgacgccagc
     7741 agcggcgcac gctgtaagga tcaggccacc ggcaactggt atgttcgcaa cgtcacccat
     7801 acgaaagcag caaatctgcg gttaataaat acccactcgc tggcggaggt atttatcaac
     7861 agcgacggag taccgactct gggcgaaggg aacgccgact gccggacaca gaccatcggc
     7921 agcctttcag gattaagttg taagatggtc aactataccc tgcaaacaaa cggactcagc
     7981 aacacctcaa tccatatatt cccggcgatc gccaactcgt cgttagcctc ggccgtcggg
     8041 gcgtacgata tgcagttcag tctgaatggc agttcatgga aaccggtgag caataccgcc
     8101 tattactaca ccttcaacga gatgaagagc gcagactcga tctatgtttt cttctcgagc
     8161 aacttcttta agcagatggt gaacctcggg atcagcgata tcaacaccaa agatctattc
     8221 aactttcgct ttcagaacac cacatcaccg gagtctggct ggtatgaatt ttctacctcc
     8281 aacacgctga ttatcaaacc ccgtgatttc agcatcagta ttatctccga tgaatatact
     8341 cagacaccgt cgcgggaggg atatgttggc agcggcgagt cggcactcga tttcggctat
     8401 atcgtaacca ccagcggtaa aacagctgcc gacgaagtgc tgatcaaggt gaccggaccc
     8461 gcgcaggtga ttggcgggcg ctcctattgt gtcttcagct ccgatgacgg taaggcgaaa
     8521 gtaccgttcc cggcgacact ttcctttatt acccgcaacg gagctacaaa aacctacgat
     8581 gccgggtgcg atgatagctg gcgggatatg accgatgcgc tgtggttgac cacaccgtgg
     8641 actgatatct ctggcgaagt ggggcagatg gataagacca cagtcaaatt ttcgattcca
     8701 atggataacg ccatttcgct gcgtacggta gatgataacg gctggtttgg cgaagtcagc
     8761 gcttcaggag agattcatgt tcaggcgacg tggcgtaaca ttaactaagg ccctgctgac
     8821 agcggtctgt atgctggcgg cacctttgac acaggcgatt tcggtcggca atctgacatt
     8881 ttcgctgccg tccgagactg actttgtcag caaacgtgta gtgaataaca acaaaagcgc
     8941 acggatatac cgtattgcca tcagtgctat tgatagcccg ggcagcagtg aattgcgcac
     9001 ccgaccggtg gatggtgaac tgcttttcgc cccccgccaa ctggcgttgc aggctggtga
     9061 gagcgagtat tttaaatttt actatcatgg tccacgggat aaccgcgagc gctactaccg
     9121 ggtctcattt cgcgaagtcc ccactcgtaa ccagacaaga cgtagcccaa ccggcggcga
     9181 ggtcagcacg gagccggtgg tggtgatgga taccattctg gtagtacgac cacgtcaggt
     9241 tcagtttaaa tggtccttcg acaaggtgac aggaacggta agtaacactg gcaacacatg
     9301 gtttaagcta ctgattaagc caggatgtga ttcgaccgaa gaggaaggcg atgcctggta
     9361 tctacgtccg ggagacgtgg ttcatcagcc tgagttacgt cagccgggga atcattatct
     9421 ggtctataac gacaaattca ttaagattag cgattcctgt ccggctaagc ccccttcggc
     9481 ggactaagac ttatccatcg gtcgagggaa gaattccacc tcagagctcc aaaaatcgtt
     9541 taaatgatgt ggaagcgatc gtcaatgtca cctgtaacga tgggctttct catatgcaca
     9601 ccatagtgtt caggagacac ggaaaggtat caggcagcct gctaatgagt ggtttaatca
     9661 cgaattagta cgtaaaatcg gtaacggctg gaaatcattc aatactcaca ctatcgaaag
     9721 ttccccagcc aaccgcggta cgttcttaca tacgatgtac cgctgttctc tttacgattt
     9781 ttagctgtac tggtgaatta tgagcaatct gaatccatgc atgacgtgtg gtgcctgttg
     9841 tgcatttttc cgcgtctctt tttactgggc cgaagccgac gatgctggcg gtactattcc
     9901 cgccaggctc actgaacaaa tatccccttt tcaccgatgc atgagcggta ccaatcagaa
     9961 aaacccccga tgtattgccc ttgcaggaac cccgggcaaa aatgcctgct gcacgatata
    10021 taaaaatcga tcgtccacat gcagagaatt cgccatgtct ggtgagaacg gagtcgtcaa
    10081 tgaggcttgc aatcgtgcaa gggctaaata cgggctgaca ccgttataaa catacaataa
    10141 ttaattgcac tgccccgcca gcgataatag cggggcttcg tttttcaggg gtaacaaaac
    10201 ccgatacttc ttctatttgc cagcaacaat gcctctcttc tgtagcgttc ttcggatcta
    10261 catcatcctg aagtagcgcg atatactcac tgactatctg catcagaata taaaaagcaa
    10321 tgtttttaac ctataaaaat ggtgctgtat ttgcgccatt tttatcattc aatgcattat
    10381 ctgtttgagc ctaaagggat ctcagggtct ggctcatgag taattctgtt tcgtaaatct
    10441 ctgcgaataa tttcaataga ccagaaccag actaaatgtc caaaaatttc agaaacattc
    10501 tcataccacg ggagatcaaa cagaggtggc gtcagtccca tgagagggaa tgaaatcata
    10561 tgaacaaaaa gttgggctaa agcacctgcc agtaagccct gccagagttt aatttttgga
    10621 aatacttcag cgaccacaca ataaccgaca gcaaacacta tcgaaaagat aatgtgcgta
    10681 acaccaaccc agttaaagac atgcccggca aaggtataaa cagccgcatt gggatctgtc
    10741 agccccaacc agtctcgaag aaaaatatac ggtggattga gaaaattacg cgagcaatca
    10801 atttggcctg cagccctgat taatgattcc gggccacacg ctgcattaaa catatccacc
    10861 gggctacgtg gcggcaatgg aacttcagcc ccccacttca cgaatgcgga aacaacgcca
    10921 gcaatcagcc caatgaatgc agcaagacca taacgtctgc ggttcggtgg agtttgttca
    10981 aatatattca tatctaccct gcttgtacca ttatgttata cacctcttca ggagtattca
    11041 taaaacaagg cgaatgtaaa gaactgtatt gttttgtata acaagatggt ttcctaatcg
    11101 ccaatgaata taagctccat catttctccc tatttttata ttagaagtga cagagatttg
    11161 cagggtgatg cagagctgaa aatcacaggt ttccttattg gtttttgcat cgtacaacta
    11221 aagcaataaa ccagctccca tcatcatcaa catcccggcg aacatgaatt tattagccgg
    11281 gctcatagct gcattcgcgt tttaagaata atcctcctgc tgtcgccgac tatgcttaac
    11341 atttaaaaaa gcatcagcac tctcgcaacg cactcttatt ttccccttta gaataccgga
    11401 ggcctggtat gagcaaccaa ggcgaatacc ccgaagataa tcgggttggg aagcacgagc
    11461 cgcacgattt aagtttgacc cgtcgcgatc tgattaaagt gagcgccgca acagcggcga
    11521 ccgccgtgtt ttatcctcat tctacgctgg cggcaagcgt tccggcagct acacccgcgc
    11581 cagagataat gcccctgaca ctgaaggtga acggcaaaac cgagcagctt gaggtggata
    11641 cccgaaccac gctactggac gctttgcgtg aaaatctgca tttgatcggt accaagaaag
    11701 gttgcgatca cggacagtgc ggagcctgta ccgtgctggt caatggtcgc aggcttaatg
    11761 cctgcctgac gcttgcagtc atgcatcagg gggccgagat caccaccatt gaaggcctgg
    11821 gttcgccaga taatcttcac cccatgcagg cggcctttat caagcatgat ggcttccagt
    11881 gcggctactg cacctccggg caaatttgct catcagtagc ggcgctaaaa gagattcagg
    11941 acggcattcc cagtcacgtc acggtcgatt tggtttccgc tccagaaaca actgccgatg
    12001 agatccgtga acgtatgagc ggcaacatct gtcgctgtgg tgcatacgct aacatccttg
    12061 ccgccattga agatgctgcg ggggagataa aatcatgaag gcgtttacct atgaacgagt
    12121 gaacacccca gccgaggcgg cacttagcgc tcagcgcgta cccggcgcaa aatttatcgc
    12181 gggcgggacc aatctgctgg acctgatgaa gctggaaatt gaaacgccca cccaccttat
    12241 cgatgtgaac ggcctcgggc tcgataagat tgaagtgacc gacgcgggtg ggctgcgcat
    12301 cggcgcactg gtacggaaca ccgacctggc ggctcacgag cgcgtgcgtc gtgattacgc
    12361 ggtactctcc cgcgccctgc tcgctggcgc atctggtcag ttacgtaatc aggcaaccac
    12421 cgcaggtaat ctgctccagc gcacgcgctg cccctatttt tacgacacca atcagccctg
    12481 caataagcgc ctgcccggga gcggctgcgc ggcgcttgaa ggctttagcc gtcagcacgc
    12541 ggtggtaggc gtaagtgaag cctgcatagc cacccatccg agcgatatgg cggtcgcaat
    12601 gcggttgctg gatgcggtgg tggaaaccat cacgccggag ggaaagactc gcagtatcac
    12661 actggctgat ttttatcacc caccggggaa aacgccgcac attgaaaccg ccctgcttcc
    12721 cggtgagctt atcgttgcgg tgacgttacc tccaccgctc ggcggaaaac atatctaccg
    12781 taaggtgcgc gatcgcgcct cctacgcctt tgccctggta tcggtcgcgg cgattattca
    12841 gcctgacggc agcgggcgcg tcgcgctggg cggagtagca cataagccct ggcgcattga
    12901 ggctgcggat gctcagctat cccagggggc gcaggccgta tatgacgcgc tgttcgccag
    12961 cgcccatccc accgctgaaa atacctttaa actcctgttg gcgaagcgaa cgcttgcctc
    13021 cgtactggct gaagcgaggg cacaggcatg aaatttgata aacccgcagg ggaaaacccg
    13081 atcgatcagc tgaaggttgt cggtcgtccc catgaccgca tcgacggacc gctgaaaact
    13141 accggcacgg cacgctacgc ctacgaatgg catgaagaag cccccaacgc cgcctatggc
    13201 tatatcgtcg gttccgccat tgccaaagga cgcctcaccg cccttgatac ggacgccgcg
    13261 caaaaagcgc cgggcgtact ggctgtcatt accgccagta acgccggggc actcggcaaa
    13321 ggcgacaaaa acaccgccag actgttaggc ggccccacta ttgagcacta tcatcaggcc
    13381 attgcgctgg tagtggccga gaccttcgaa caggcgcgag cggcggcctc gctggtgcag
    13441 gcacactatc gccgtaataa aggagcttac tccctggcgg acgaaaaaca ggccgtcaat
    13501 cagccgccgg aagacacgcc cgacaaaaac gtcggtgact ttgacggggc tttcacctcc
    13561 gctgcggtga agattgatgc tacctacacg accccggacc agagccatat ggcgatggag
    13621 ccgcatgcct cgatggccgt ctgggatgga aataagctta ccctctggac ctcaaatcag
    13681 atgattgact ggtgccgcac cgatctggca aaaacgctga aagttcccgt ggagaatgtg
    13741 cgtattatct ccccgtatat cggcggaggg tttggcggca agctgttcct gagaagcgat
    13801 gcgctgctgg cggccctcgc cgcccgagcg gtgaaacgtc cggttaaagt gatgctcccc
    13861 cgcccctcta ttcccaataa caccacgcac cgccccgcca cccttcagca cttgcgtatc
    13921 ggtgccgacc agagcgggaa aatcaccgct atctcacatg aaagctggtc tggaaacctg
    13981 cccggcggca cgccggaaac ggcggtacag caaagcgaat tactctacgc cggggcgaat
    14041 cgtcataccg gcctgcggct cgccacgctt gatttgccgg aagggaacgc catgcgtgcg
    14101 cccggcgaag cccccggtct gatggcgctc gaaatcgcga tcgacgaact ggcggaaaaa
    14161 gcgggcatcg atcccgtcga gtttcgcatc ctgaatgaca ctcaggttga ccccgccgac
    14221 ccgacgcgct gcttctctcg ccgtcagctt atcgagtgct tgcgcaccgg agcggataaa
    14281 tttggctgga agcagcgcaa cgccacaccc ggacaggtgc gcgacgggga gtggctagtc
    14341 ggccacggtg ttgcggcggg ctttcgcaat aatctgctgg aaaaatcggg tgctcgggtt
    14401 cacctcgaac aaaacggcac cgttaccgta gaaacggaca tgaccgacat tggcaccggc
    14461 agctacacca ttctggccca gacggcagcg gaaatgcttg gcgtaccgct ggagcaggtt
    14521 gcggttcacc tcggcgattc cagtttcccg gtttctgcgg gttctggtgg acaatggggc
    14581 gcgaatacct ccacctccgg cgtttacgcc gcctgtatga agcttcgcga aatgattgcc
    14641 tcggcagtcg ggtttgatcc tgagcagtcg cagtttgccg acggcaagat taccaacggt
    14701 acccgaagcg ccacgctaca tgaagccacc gcaggcggca gactgacagc ggaagagagc
    14761 attgaattcg gaacactgag caaagagtac cagcagtcga cctttgccgg gcattttgtg
    14821 gaggtcggcg tgcatagcgc gacgggagaa gttcgggtcc ggcgtatgct cgctgtgtgt
    14881 gctgcaggac gcatcctgaa tccgaaaact gcgcgcagcc aggtcattgg cgcaatgact
    14941 atgggcatgg gcgcggcact gatggaggag ctggcggtgg atgaccgttt gggctacttc
    15001 gttaatcacg atatggcggg gtatgaggtg ccggtccatg cggatatccc aaaacaggag
    15061 gtgattttcc tggatgatac cgaccccata tcctccccga tgaaggccaa aggtgtcggt
    15121 gagttgggcc tgtgcggcgt gagcgcggct atcgccaacg cggtgtataa cgccaccggt
    15181 attcgggtac gggattatcc catcactctg gataagctgc tcgataagct gccggatgtg
    15241 gtttaaggag aaacaatgtc atacccgctt tttgataaag acgaacactg gcataagcca
    15301 gagcaggcgt ttctcaccga tgaccaccgg accattctgc gcttcgccgt agaggcgcta
    15361 atgtccggta aaggagcggt gctggtgacg ctggtggaga tacacggcgg cgcggcgcgc
    15421 ccgctcgggg cgcagatggt ggttcgcgaa gatggtcgtt actgcggttt tgtctctggc
    15481 ggctgcgtgg aggccgctgc cgcttttgaa gcgctggaga tgatgggctc aggccgcgat
    15541 cgcgaaattc gctatggcga aggttcgccg tggtttgaca tcgttctgcc ctgcggcggt
    15601 gggatcacgc ttacgctcca taaactacgc tcggcacagc ctctgctcgc cgtgctgaac
    15661 cgtctggaac agagaaaacc ggcggggctg cgctacgatc cgcaagcaca atcgttggtg
    15721 tgcctgccca cgcaaacccg aacgggctgg aatctcaatg gctttgaggt ggggttcagg
    15781 ccatgcgtca ggctgatgat ttacggacgt tctcttgagg cgcaggcaac cgcgagtctt
    15841 gcagcagcca caggctatga cagccatatc ttcgatcttt ttccggcctc agccagcgct
    15901 cagatcgata ccgacacggc ggtcattttg ctgtgccatg atctcaaccg ggagctgcca
    15961 gcattgcagg ccgcgcgaga agcaaagccc ttttatctcg gcgcattggg cagccatcga
    16021 acccacacgt tacgtctgca aaagctccac gagctgggat ggtccaggga ggagacagcg
    16081 caaatccggg cccccgtcgg gatattcccc aaagcccggg atgcgcatac tctggcactc
    16141 tccgtgctgg cagaaatcgc ctctgtacgt ctccatcatg aggaggattc atgcctgccc
    16201 ccgtcgtcct gatccttgcg gccgggcgtg gagagcgctt tctcgcctcc gggggaaata
    16261 cccataagtg tatcggctgg cgtcagtccc cggaggttgc gccttatcgc tggccatttg
    16321 aagaaaacgg gagaactttc gaccttgcga ttgaaccgca gattacgact aatgatctgc
    16381 gtctgatgtt gaggctggct cttgccggcg gaggaataac aattgccact caggaaactt
    16441 tcaggccata tattgaaagc ggtaagcttg tatcgctgct tgatgacttt cttccacaat
    16501 ttccgggctt ctatctgtat ttcccacagc gtcgcaatat tgcaccaaag ctccgcgccc
    16561 tgattgacta cgtcaaagaa tggcggcagc aattggttta aatagctgca cctgcactgc
    16621 ctgatgtcag aacagtattt tgatgaattg ccaaggttac aatggcacaa atgcggcaca
    16681 ggaggaaacg tggtgtattt aaatatgggg taactctttg attttaatgg tgccgataat
    16741 aggagtcgaa cctacgacct tcgcattacg aatgcgctgc tctaccaact gagctatatc
    16801 ggccctgaaa ggacatgttc acgaacgtga atcacggtgg acaaggttaa aactaaccgg
    16861 gcgatgcgtc aatggccttg tgaatcaaat ggctactttt gcatcacccg gttttattta
    16921 cgcacgaatg gtgtaatcac caatgccgat ccacttgtaa gtggtcagtg cttccagccc
    16981 cattgggcca cgcgcgtgga gtttttgtgt gcttaccgcc acttccgcac ccagaccaaa
    17041 ctggccgccg tcggtaaaac gcgtagaggc gttaacgtaa acagcggacg aatccacttc
    17101 gttaacaaaa cgctgggcgt tgcgcatatc gcgggtcagg atcgcatcgg agtgttgtgt
    17161 gccgtgttca cgaatatggg cgatggcatc gtcaagatcg ctgacgattt taacgttcaa
    17221 atctaatgac agaaattcat cgtcatactc ttcggcttta accgccacca ccttcgcagg
    17281 gcctgtctgc aactgcgcca gcgcagctgc atctgcgtgt aatgtcacgc cgctttccgc
    17341 catttgtttg cttaatgcgg gcaggaagct atcggcgatg tttttattca ccagcaacgt
    17401 ttctaccgta ttacatgtgc tcggtcgctg agttttcgcg ttgacgatca cttttaatgc
    17461 ttcagcgatc tctgcgcttt catcaacgta aatatggcat acgcctatac cacctgtgat
    17521 caccgggatt gtcgactgtt cgcggcacag tttatgcaaa ccagccccgc cgcgcgggat
    17581 cagcatgtcg atgtatttat ccatacgcag catttcactg accagcgcac ggtcaggatt
    17641 atcaatcgcc tgcacggcac ccgccggtaa gccacaggat ttcagggcgt cctgaatcac
    17701 cgccaccgtt gccgcgttag tgcgacaggt ttctttgcca ccacgcagga tcaccgcatt
    17761 accggttttc aggcacagcg aagcgacatc aaccgtcacg ttcgggcgcg cttcataaat
    17821 cacgccaatc acccccagcg gcacgcgacg acgctcaaga cgcaggccgc tgtccagtac
    17881 gctgccatcg attacctgcc ccaccggatc ggcgaggtta cacacctggc gcacatcatc
    17941 ggcaatgcct ttcagccgtg cgggcgtcag tgccagacgg tcaagcatcg cttcgccaag
    18001 gccattggca cgcgcgtcag caacatcctg ggcgttagcg ttgaggatga tttcgctttg
    18061 tgcttccagt tcatcggcga ttttttccag cacgcgattt ttttcgcggc tggagagttg
    18121 cgctaattta tacgaggctt gcttcgcggc aatgcccatt tgttccagca tcagcctgct
    18181 ccttaacggg taatcatgtc atcacggtga acggcaaccg ggccgtattc atatcccagt
    18241 attgcatcaa tttcttgcga gtggtgcccg gcaatacggc gtaatgcatc gctgttgtaa
    18301 cgactgacgc cgtgggcgat atcgcgacct tcgaggttgc aaatgcggat gacttcacca
    18361 cgcgagaaat tgccagtcac gcttttaatg cctttcggca acagggagct gccgcgttca
    18421 agaatggcgg cagttgcccc ttcatctacc gtgatttcac ccgccggcgg cgcaccgaaa
    18481 atccagcgtt tacggttttc aagcggagtc gcctgggcat ggaacagcgt accgacggaa
    18541 atgccttcca tcacatcacc aataacgccc ggcttgctgc ccgcggcaat aatggtgtcg
    18601 atacccgcac ggcaagccac gtcagcggcc tgcaatttgg tactcatgcc gccagttccg
    18661 aggcctgaaa cgctgtcacc ggcaatcgcg cgcagtgcgt catcaatgcc gtaaacatct
    18721 ttaatcagtt ctgcctgcgg attgctgcgc ggatcagcgg tatacaaacc tttttgatcg
    18781 gtcagcagca acagtttatc ggcacccgcc agaatcgccg ccagcgcaga aaggttatcg
    18841 ttatcgccga ccttaatctc tgccgtagcg acagcatcgt tctcattgat taccggaacg
    18901 atattgttat cgagcaacgc acgcagggtg tcgcgggcgt tcaggaagcg ttcacggtct
    18961 tccatatcag cacgggtcag cagcatctgc ccgacgtgaa tgccataaat cgaaaacagc
    19021 tgttcccaca gttgaatcag tcgactctgc cctaccgccg ccagcagttg tttcgaggcg
    19081 atagtcgctg gcagttccgg gtaccccagg tgctcacgtc cggcggcgat cgcgcccgac
    19141 gtcacaataa caatccgatg cccggcggca tgtaactgcg cgcactggcg aacaagttca
    19201 acgatatggg cacggttcag acggcgcgat ccgcctgtta gcacactggt gccgagtttt
    19261 accaccagcg tctggctgtc actcatgatt ctctgccatt caattttagg aaaaatgata
    19321 tcaaacgaac gttttagcag gactgtcgtc ggttgccaac catctgcaag caaagcatgg
    19381 cgttttgttg cgcgggatca gcaa
//
LOCUS       DAZFQD010000099        19315 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.099, whole genome shotgun sequence.
ACCESSION   DAZFQD010000099 DAZFQD010000000
VERSION     DAZFQD010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19315
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.099"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(46..447)
                     /gene="crl"
                     /locus_tag="ACHKTW_003475"
     CDS             complement(46..447)
                     /gene="crl"
                     /locus_tag="ACHKTW_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HFO6464428.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(505..1749)
                     /gene="frsA"
                     /locus_tag="ACHKTW_003476"
     CDS             complement(505..1749)
                     /gene="frsA"
                     /locus_tag="ACHKTW_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HFO6464429.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDDA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKALQEITGWIEKRLC"
     gene            complement(1841..2299)
                     /gene="gpt"
                     /locus_tag="ACHKTW_003477"
     CDS             complement(1841..2299)
                     /gene="gpt"
                     /locus_tag="ACHKTW_003477"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFO6464430.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            2560..4017
                     /gene="pepD"
                     /locus_tag="ACHKTW_003478"
     CDS             2560..4017
                     /gene="pepD"
                     /locus_tag="ACHKTW_003478"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HFO6464431.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDVL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            complement(4074..4688)
                     /gene="prfH"
                     /locus_tag="ACHKTW_003479"
     CDS             complement(4074..4688)
                     /gene="prfH"
                     /locus_tag="ACHKTW_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HFO6464432.1"
                     /translation="MILLQLSSAQGPEECCLAVKKALDRLIKEAARQDVAVTVLETET
                     GHYSDTLRSALISLDGDNAWALSESWCGTIQWICPSPYRPHHGRKNWFLGIGRFTADE
                     QEQSDAIRYETLRSSGPGGQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLL
                     IAWKLEQQQQENSAVLKSQRRMFHHQIERGNPRRTFTGMAFIEG"
     gene            complement(4685..5794)
                     /locus_tag="ACHKTW_003480"
     CDS             complement(4685..5794)
                     /locus_tag="ACHKTW_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HFO6464433.1"
                     /translation="MGNYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSVGRFYPALVGNDIGCGMALWQTDILTRKYNADKFEKRLSD
                     LDDVAEESWLEENLPSAFAQHPWRSSLGSIGGGNHFAELQQVDQIINAELFALAGLDA
                     QHLQLLVHSGSRGVGQSILQRHIASFSHHGLPQGSDDALAFACINRQMIALRIMQQVK
                     ATGSPVLDVAHNFVSACQIGDQQGWLHRKGATPDDNGLVIIPGSRGDYSWIVQPVANE
                     KTLHSLAHGAGRKWGRTECKGRLAAKYTATQLSRTELGSRVICRDKQLIFEEAPQAYK
                     SAESVVQCLVQAGLIIPVARLRPVLTLKNSGGKKG"
     gene            complement(6040..6492)
                     /gene="yafP"
                     /locus_tag="ACHKTW_003481"
     CDS             complement(6040..6492)
                     /gene="yafP"
                     /locus_tag="ACHKTW_003481"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO6464434.1"
                     /translation="MNNIQIRDYQPGDFQQLCAIFIRAVTMTASQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAKLVGFITCVEHYIDMLFVDPEYTRRGVASALLKPLIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGEWFINFYMRYKQQH"
     gene            complement(6489..7544)
                     /gene="dinB"
                     /locus_tag="ACHKTW_003482"
     CDS             complement(6489..7544)
                     /gene="dinB"
                     /locus_tag="ACHKTW_003482"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFO6464435.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELHLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVILLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            complement(7615..8400)
                     /gene="lafU"
                     /locus_tag="ACHKTW_003483"
     CDS             complement(7615..8400)
                     /gene="lafU"
                     /locus_tag="ACHKTW_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HFO6464436.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFSGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAQIMPFFKTLLVELAPVFDSLDNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDAKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRMR"
     gene            8372..10084
                     /locus_tag="ACHKTW_003484"
                     /pseudo
     CDS             8372..10084
                     /locus_tag="ACHKTW_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140831.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071978 - bacterial-type
                     flagellum-dependent swarming motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            10189..10467
                     /locus_tag="ACHKTW_003485"
     CDS             10189..10467
                     /locus_tag="ACHKTW_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000598761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HFO6464437.1"
                     /translation="MIKVSALGSRHRKTFDDVMTEPPKSGIKWDDVVALIKAVGGTIK
                     NNNGSRRKFQIGTTKFSTHEPHPKNVMDKGAVAGLKEWFVNCVGVDYE"
     gene            10460..10816
                     /locus_tag="ACHKTW_003486"
     CDS             10460..10816
                     /locus_tag="ACHKTW_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFO6464438.1"
                     /translation="MNKASNIITIKGQPAAVTFEADINAFRGKFLNVNGYCDFVATSI
                     EALYREGESALDEWMADCEEDGIAPFREEEEQKRLTLRVPHHIDSRLTIVARQHSISK
                     NQLIVDVLEREFSAQM"
     gene            complement(10873..11646)
                     /gene="yafL"
                     /locus_tag="ACHKTW_003487"
     CDS             complement(10873..11646)
                     /gene="yafL"
                     /locus_tag="ACHKTW_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308281.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFO6464439.1"
                     /translation="MHDQPLLSMSLPSIPSFVLSGLLLLCLPFSSFASATTSHITFSY
                     AARQRMQNRARLLKQYQTHLKKQASYIVEGNAESRRALRQHNREQIKQHPEWFPAPLK
                     ASDRRWQALAENNHFLSSDHLHNITEVAIHRLEQQLGKPYVWGGTRPDQGFDCSGLVF
                     YAYNKILEAKLPRTANEMYHYRRATIVANNDLRRGDLLFFHIHSREIADHMGVYLGDG
                     QFIESPRTGEIIRVSQLAEPFWQDHFLGARRILTEETIL"
     gene            11832..12092
                     /gene="dinJ"
                     /locus_tag="ACHKTW_003488"
     CDS             11832..12092
                     /gene="dinJ"
                     /locus_tag="ACHKTW_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin DinJ"
                     /protein_id="HFO6464440.1"
                     /translation="MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVARE
                     KALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDLFDKLGI"
     gene            12095..12373
                     /gene="yafQ"
                     /locus_tag="ACHKTW_003489"
     CDS             12095..12373
                     /gene="yafQ"
                     /locus_tag="ACHKTW_003489"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafQ"
                     /protein_id="HFO6464441.1"
                     /translation="MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLP
                     AVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLRFERTGTHAALFG"
     gene            12529..13269
                     /gene="dpaA"
                     /locus_tag="ACHKTW_003490"
     CDS             12529..13269
                     /gene="dpaA"
                     /locus_tag="ACHKTW_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan meso-diaminopimelic acid protein
                     amidase"
                     /protein_id="HFO6464442.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVVN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            complement(13240..14007)
                     /gene="yafJ"
                     /locus_tag="ACHKTW_003491"
     CDS             complement(13240..14007)
                     /gene="yafJ"
                     /locus_tag="ACHKTW_003491"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HFO6464443.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRQKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(14213..14791)
                     /gene="lpcA"
                     /locus_tag="ACHKTW_003492"
     CDS             complement(14213..14791)
                     /gene="lpcA"
                     /locus_tag="ACHKTW_003492"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HFO6464444.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            15031..17475
                     /gene="fadE"
                     /locus_tag="ACHKTW_003493"
     CDS             15031..17475
                     /gene="fadE"
                     /locus_tag="ACHKTW_003493"
                     /EC_number="1.3.8.7"
                     /EC_number="1.3.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0033539 - fatty acid beta-oxidation using
                     acyl-CoA dehydrogenase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
                     /protein_id="HFO6464445.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLAIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKNHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYAHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMAVLGGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDDLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKVAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQ
                     RICKELGKNLPFTRLDELAHNALAKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            complement(17518..17964)
                     /gene="ivy"
                     /locus_tag="ACHKTW_003494"
     CDS             complement(17518..17964)
                     /gene="ivy"
                     /locus_tag="ACHKTW_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HFO6464446.1"
                     /translation="MFKAITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     TIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            18145..18915
                     /gene="yafV"
                     /locus_tag="ACHKTW_003495"
     CDS             18145..18915
                     /gene="yafV"
                     /locus_tag="ACHKTW_003495"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HFO6464447.1"
                     /translation="MPGLKITLLQQPLVWMNGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAL
                     YVANWPAPRSLHWLALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHQATRIDAELSMAVLREYRDKFPAWRDADEFRLR"
     gene            complement(18957..>19315)
                     /locus_tag="ACHKTW_003496"
     CDS             complement(18957..>19315)
                     /locus_tag="ACHKTW_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HFO6464448.1"
                     /translation="CVAVSFRSIIAEQKKEPEMTVRYYISSADLTAEKFATAIRNHWH
                     VENKLHWRLDVVMNEDDCKIRRGNAAELFSGIRHIAINILTNDKVFKAGLRRKMRKAA
                     MDRNYLASVLAESGLS"
BASE COUNT         4656 a         4978 c         4971 g         4710 t
ORIGIN      
        1 gttttaatga ttattgccgg atgtgatgca tccggcacat ttcactcacg ccgttaactt
       61 caccggctcg tcacgaaaat catccgccgg ttccagcttc agattcagcg tcgtcagcag
      121 ttcacgcagc ttctcgtgaa actcacgcag ggtgtgctcc agtcgttcaa ccacttcagt
      181 gtcttttacc ggaacgctct tccagtcgcc tgctttatcg aacagaccaa actggtagct
      241 gtaggtaaaa cgggattcct gcgcttccag ctccatccac cagccccaga attcacgcac
      301 ttccggtgcc ggtttcacgt tgacgcatac agccagacaa tcgaaaaaga atcgattatc
      361 ttcgcactta ccttcacgaa tatacgggcc tagtgcggta aattttttga tcaatctgct
      421 cttcgggtgt ccactcggta acgtcattgc tatctcctgt tgtgatgcaa ctgttttacc
      481 aaattggcaa aatttagcaa atttttaaca caagcgtttt tcgatccagc cggtgatttc
      541 ctgaagagct ttgtcaaaat tccgatacac cgggttaaat gggatctcta ataatttacc
      601 gtcagcagat gatgaggtga ttaagcgtga gtcctcttcc gggctgaacg gatcgttctt
      661 ccagtagcct gataacattg gcgttgggca gcgacgtccc agcaatcctt gcacttttaa
      721 tgaatagcga ttcagctcca cgcgcaacgc gtcatcggaa gcatcatgca tccccaaacg
      781 actcgccaga acgtcaagat acatttccgg cacctgttgc tggcacttaa aatcactcaa
      841 cagggtatga actaccggac caagacaggc aaccgctttc agacgcggcg attcaaggta
      901 tgccagacgc acggcaacgt tagcgccgaa acggaaacca aaggccgcga cgcgagtgtg
      961 atccacccac ggtacgttag gcagcgcctt taagacgtgc tgatgcaaca ggctggagtc
     1021 ctgggtgagc ttccattttg aagaaaagcc caccgacggc atatcaatag tcagcatcgc
     1081 aatgccgcgc ggcgcaaaat aacgttcata caggctgtaa tagtccgtct gcatcgcatc
     1141 cagaccacca cacattaata ctgtcgggaa cgggccatcg cctttcggca tatgcaaaaa
     1201 gccggtgatg ggcgcaccgc cgggtacggt aaactccatc tgccgcatcg tgcccggcag
     1261 acgctgggcg gcctcttcat aggcgcggtt tgataaagcc tgcgcttgct cggccaggtc
     1321 atctcctttc agatgaggat aggcggcaat gttgtacaac gtagccgcat gcagccagtg
     1381 tcgaccactg agttgcggat cttcttccgc acaggctttt tgttgccaca ccatcgcctg
     1441 ggtggcccac tcataaatcc agttgccacc acggtagcca atcacggtat cgtataaatc
     1501 atcatcggta cgttcagcat cgctcatcac aatacgtgcc tggacgtcga ggatttcacg
     1561 tgggtcaatg ccgcgccaga tccacatcag acggttaatc atgcgatacc agtgagggat
     1621 ggttttacca tcaagagccg actgcacagg cggttgtgcg ccgtgattaa agcggcggac
     1681 tagcgtcgag gtttcaggat gtttaaagcg gggtttgaac agggtttcgc tcaggtttgc
     1741 ctgtgtcatg gatgcagcct ccagaatact tactggaaac tattgtaacc cgcctgaagt
     1801 taaaaagaac aacgcccggc agtgccaggc gttgaaaaga ttagcgaccg gagattggcg
     1861 ggacgaatac gacgcccata tcccacggct gttcgatcca ggtatcttgc gggatatcaa
     1921 caacatagtc atcaaccagc ggacgaccag ccggttttgc gaagatagtc acaaaatgtg
     1981 cttttggata catttcacga atcgcaaccg cagtaccacc ggtatccacc aggtcatcaa
     2041 taacgatgaa gccttcgcca tcgccttctg cgcgttttag cactttaagc tcgcgctggt
     2101 tgtcgtgatc gtagctggaa atacaaacgg tatcgacatg acgaataccc agttcacgcg
     2161 ccagtaacgc acccggtacc agaccgccac ggcttacggc aataatgcct ttccattgtt
     2221 cagaaggcat cagtcggctt gcgagtttac gtgcatggat ctgcaacatg tcccaggtga
     2281 cgatgtattt ttcgctcatg tgaagtgtcc cagcctgttt atctacggct taaaaagtgt
     2341 tcgaggggaa aataggttgc gcgagattat agagatctgg cgcactaaaa accagtattt
     2401 cacatgagtc cgcgtctttt tacgcactgc ctctccctga cgcgggataa agtggtattc
     2461 tcaaacatat ctcgcaagcc tgtcttgtgt tgacaacatt ttctgctaac cctgtgacct
     2521 gcaatactgt tttgcgggtg atcgacaagg agacttaacg tgtctgaact gtctcaatta
     2581 tctccacagc cgctgtggga tatttttgcc aaaatctgtt ctattcctca cccgtcctat
     2641 catgaagagc aactcgctga atacattgtt ggttgggcaa aagagaaagg tttccatgtc
     2701 gaacgcgatc aggtaggtaa tatcctgatt cgtaaacctg ctaccgcagg tatggaaaat
     2761 cgtaaaccgg tcgtcttaca ggcccacctc gatatggtgc cgcagaaaaa taacgacacc
     2821 gtgcatgact tcacgaaaga tcctatccag ccttatattg atggcgaatg ggttaaagcg
     2881 cgcggcacca cgctgggtgc agataacggc attggtatgg cgtctgcgct ggcggttctg
     2941 gctgacgaaa acgtggttca cggcccgctg gaagtgctgc tgaccatgac cgaagaagcc
     3001 ggtatggacg gtgcgttcgg cttacagagc aactggttgc aggctgatat tctgattaac
     3061 accgactccg aagaagaagg tgaaatctac atgggttgtg cggggggtat cgacttcacc
     3121 tccaacctgc atttagatcg tgaagcggtt ccagctggtt ttgaaacctt caagttaacc
     3181 ttaaaaggtc tgaaaggcgg tcactccggc ggtgaaatcc acgttggcct gggtaatgcc
     3241 aacaaactac tggtgcgctt cctggcgggt catgcggaag aactggatct gcgccttatc
     3301 gatttcaacg gcggcacact gcgtaacgcc atcccgcgtg aagcctttgc gaccattgct
     3361 gtcgcagctg ataaagtcga cgtcctgaaa tctctggtga atacctatca ggagatcctg
     3421 aaaaacgagc tggcagagaa agagaaaaat ctggccttgt tgctggactc tgtagcgaac
     3481 gataaagctg ccctgattgc gaaatctcgc gataccttta ttcgtctgct gaacgccacc
     3541 ccgaacggtg tgatccgcaa ttccgacgtg gcaaaaggtg tggtcgaaac ctccctgaac
     3601 gtcggtgtgg tgaccatgac tgacaataac gtagaaattc actgcctgat ccgttcactg
     3661 atcgacagcg gtaaagacta cgtggtgagc atgctggatt cgctgggtaa actggctggc
     3721 gcgaaaaccg aagcgaaagg cgcatatcct ggctggcagc cggacgctaa ttctccggtg
     3781 atgcatctgg tacgtgaaac ctatcagcgt ctgttcaaca aaacgccgaa catccagatt
     3841 atccacgcgg gcctggaatg tggtctgttc aaaaaaccgt atccggaaat ggacatggtt
     3901 tctatcgggc caactatcac cggtccacac tctccggatg agcaagttca catcgaaagc
     3961 gtaggtcatt actggacact gctgactgaa ctgctgaaag aaattccggc gaagtaatta
     4021 tttgatttgc tgccggatgg cgtttaatcg ccttccggca gtttcatcct tcattatcct
     4081 tcgataaaag ccatccctgt aaatgtccgt ctcgggttgc cacgttcaat ctggtgatgg
     4141 aacattcgcc gctgcgattt cagcaccgcg ctattttcct gttgctgctg ctccagcttc
     4201 caggcaatca gcaatcgtgc cagccgcttg ttagcatgct gactacgctc tgactgaacc
     4261 ttcacgctaa taccggatgc caaatgcgtg gcgcgtaccg ccgagtcggt tttattgaca
     4321 tgttgaccgc ccggccccga cgaacgcaac gtctcataac ggattgcatc cgattgttcc
     4381 tgctcatcag cggtaaaacg cccaatgccc agaaaccagt ttttgcgccc atgatgaggc
     4441 cgatacggac tcggacaaat ccactgaata gtgccacacc agctttcgct taatgcccat
     4501 gcgttatcgc catccagaga aatcagcgcc gaacgcagcg tgtcagagta gtggcccgtt
     4561 tctgtttcca gcaccgttac cgcgacgtct tgtcgggcag cttctttaat tagcctgtcc
     4621 agcgcctttt tcactgcgag acaacattct tccggcccct gcgctgagga gagttgtagc
     4681 aagatcatcc ttttttccct ccactgtttt tgagcgttag caccggacgc agccgcgcga
     4741 caggaataat taaaccggcc tgcaccagac attgcaccac gctttcagcc gatttatagg
     4801 cttgtggcgc ttcttcaaag atgagttgtt tatcgcgaca aattacccga ctgccaagtt
     4861 cagtacgcga gagttgcgtc gccgtgtatt tcgctgccag acgcccttta cactcggtgc
     4921 gcccccattt acgccctgcc ccatgcgcca gcgagtggag cgttttttcg ttcgcaacag
     4981 gctgtacaat ccaggagtaa tcaccacgtg aaccgggaat aatcaccaga ccgttgtcat
     5041 ccggcgtggc acctttacga tgcaaccagc cctgttgatc gccgatttga cacgcgctaa
     5101 caaagttatg cgccacatcc agaaccggac ttcccgtggc cttaacttgt tgcattatgc
     5161 gcaaagcgat catctggcga ttaatacacg caaacgccag cgcgtcgtca ctaccttgag
     5221 gcaaaccatg atgcgagaac gaggcaatat gccgctgtaa aatagactgc cccacccccc
     5281 gcgagccgct atgaaccaac agttgcagat gttgtgcatc cagacctgcc agtgcaaaca
     5341 gttcagcgtt gatgatttga tcaacttgtt gcagttcagc gaaatgattg ccgccaccga
     5401 tagagccaag cgagctgcgc cacggatgct gtgcaaatgc tgacggcagg ttttcctcca
     5461 gccagctttc ttcagcaacg tcatccagat ctgataatcg cttttcaaac ttatcggcgt
     5521 tgtatttgcg agtgagaata tctgtttgcc atagcgccat accgcagccg atatcattgc
     5581 cgaccagtgc cggataaaaa cgaccaacag agaagaacgc tgcgccaatc ggatagccgc
     5641 gtccgggatg taaatctggc atccccacta cgcgctgcat gttgggtaaa tttgccgtgg
     5701 tgtgtaattg ttggatcgct aaactctcga tccacaggtc atcagatgcg atggtaaata
     5761 ccgcatcgga taagggacga atataattgc ccatatacca ttccgtaaat agactcgtca
     5821 tacttcaagt tgcatgtgtt gcgacttgaa ttattgagag taacaattaa aaaatcagga
     5881 aatggcagac gaatccaggg caagaaaaaa agaattaatc ctggaaaggt ctgcaaagag
     5941 tcattgataa aggatttgtc atgatttacc tccttgcagt ttgtggaata agaaaacgcg
     6001 cacatactag gcttgcgtta ttttcattgc aagctggatt taatgttgct gtttataacg
     6061 catataaaaa ttaataaacc actctccccg acattcaacg cgttgctgct taactgtctg
     6121 aaaaccataa cgttcaaaaa agggttttgc ggttatgctt gcgtccaccg taagttcgga
     6181 ttcagactta atcaaaggtt ttaacaaagc gctggcaacc ccacggcggg tgtattcagg
     6241 gtcaacaaat aacatatcga tatagtgttc aacgcaggta ataaagccga ccagttttgc
     6301 attaatgact gcaacccgca cttgtgattt cgcgagtttc tccttccagc gagattcgtc
     6361 aatctgcgcc caggcggcaa tttgttgtgg tgaataatgc tgactggcag tcatcgtaac
     6421 cgctctaatg aaaatagcgc atagttgctg aaaatcgcca ggctgatagt ctcttatttg
     6481 tatgttattc ataatcccag caccagttgt ctttccattt gcgggtcaag caacgtcaca
     6541 tgcagcccca ccagtcgcac accgcgcccg ccgcggcgtt catcccaggt tttacgcgcg
     6601 gtggcgatta agtcagcttt attcagccgc ggccagacgt gctcctgagt ggtttgctga
     6661 aaatcgtcga actttaattt caccccctgg cgggcaatca gtaaatcagg ttttaccttt
     6721 gccagacggc gttcaagttc cggatacagc cgctcgataa tcgcttcaca ttcagaccag
     6781 tggtggatat cttccgccat cgtgcgttcc acgccgacgg attttcgcaa ccgttcgctg
     6841 ttaacgtcgc gctcgtcaat cccctgacta cgctcccaca aaatgcggcc aaatttgcca
     6901 aagcgtttaa gcagaatcac cagatcacac ttttgcacat caccgcaggt ccgcagcccc
     6961 atcgcttcca gttttgccgc cgagactttg ccgacgccgg ggatttttgc caatggtaag
     7021 gtttgcagaa atgccggaac ttctgccgga gtaatcacaa actggccgtt gggtttattc
     7081 atgtcggagg cgattttggc gagaaacttg accggcgcaa cgcccgcaga cgccgtcagg
     7141 tgcagctcgt tgaaaatcgt ctggcggatt tcctgggcga tgagggtcgc agaaccgtgg
     7201 caatggacgc tatcggtgac atcgagataa gcctcatcca gtgacaacgg ttcaatgcgc
     7261 gaggtgtagc gcgagaagat ttcacggata tgatttgagg cttctttgta ggcgtcaaag
     7321 cgccccggaa gcaaggtgag atgtgggcat aatttgagcg ccatcccagt aggcatagcg
     7381 ctacgtacgc caaatttacg cgcgggataa ttggcggtgc tgatcacccc ccgacgttcg
     7441 cggctgccgc caatagcaat agggatatcg cgcagggcgg gattgtcgcg catctccact
     7501 gcggcgaaaa agcagtccat atccacatga atgattttac gcattgctca cctctcaata
     7561 ctggtaaagt atacagtgat ttcagggttt gagaaatgcg taaagattca gcatttagcg
     7621 cattcgctgc gaaagcacct gctgcttatc cagcttttgc accagcggct gcaccacttt
     7681 atccccatgc tgaccaaagt attgatacag cgtatcggac gcacttttgg tcagcaccat
     7741 aatctcaata cgccgattgc ccgcgctttg cggatttttg gcatccagca gcatctggtc
     7801 cgccattgcg cttacctgca tcactttatc ttccggcatt ccggcctctt ccagcacccg
     7861 acgagctgaa agcgcgcgat cgcccgaaag gttccagttg ttgtagatat tgtttttgta
     7921 ggccatcgca tcggtatgcc cggtaataat aattttgtta tcgagcgagt cgaacactgg
     7981 cgcgagctcc accagcaaag ttttaaagaa cggcataatc tgcgcgctgc cgcgttcgaa
     8041 catattgcgg ttctggtcat ctttaatcag cacgcgcaat ccctggggaa caatctccat
     8101 ctccagattc gcttccatat gcgcatcacg ggcaatggtg ttgatactgg ttgccagctc
     8161 gccaagctcg gtggcggatt tcttcttcag cagcgctgtt ttctcgttga cgtctcgcgc
     8221 ttttttctcc gtctcttccg gcaccgccac ggtggcaggt tttggcggat gagacgggct
     8281 gattttattt aacggcgaca atccgccgcc actgaaaatc gactgcccgt gcagcgcggc
     8341 gataatgctt tcacgctcgg atttactgac gctgttgaca atcaacttta ttgtcgtcac
     8401 caaaggggcc gagcgtattt ccgaggtttc agcccgcttt accctggatg cgatgcccgg
     8461 taagcagatg gcgattgacg ccgatcttaa tgccgggttg atcaatcagg cgcaggcaca
     8521 aacccgacgt aaagatgttg ccagcgaggc cgatttctac ggcgcgatgg acggggcatc
     8581 gaaatttgtg cgcggggatg ctatcgccgg gatgatgatt ctggcgatca acctgatcgg
     8641 cggcgtctgt atcgggatct tcaaatacaa cctgagcgcc gacgccgcct tccagcagta
     8701 tgtgctgatg accatcggcg acgggctggt ggcgcaaatc ccttccctgc tactctccac
     8761 cgcggcggcg attatcgtca cccgcgtcag cgacaacggc gatatcgccc atgacgtgcg
     8821 tcaccagttg ctggcaagcc catcggtgct ctacaccgct accggaatta tgttcgtact
     8881 ggcggtagtg cctggaatgc cacatctgcc gtttttgctg ttcagcgccc tgcttggctt
     8941 taccggctgg cggatgagca aacgcccgca ggcggcggaa gcggaagaga aaagtctcga
     9001 aacgctgacc cgcaccatca ccgaaaccag cgagcaacag gtcagttggg aaaccattcc
     9061 gctgatcgag cccatcagct taagcctcgg ctacaagctg gtggcgctgg tggacaaagc
     9121 ccagggcaac ccgctcaccc agcgtattcg cggcgtgcga caggtgatat ccgacggcaa
     9181 cggcgtgctg ctgccggaga tccgcattcg ggaaaacttc cgccttaagc ccagccagta
     9241 cgctattttc atcaacggca ttaaggctga tgaagcggat attccgacgg ataaactgat
     9301 ggcgctgccc tccagcgaaa cctacggcga gatagacggc gtgctgggga acgacccggc
     9361 gtacgggatg ccggtgacct ggatccagcc tgcgcagaaa gcgaaggcgc tgaatatggg
     9421 gtatcaggtg atcgacagcg ccagcgtgat tgctacccat gtaaataaga ttgtgcgcag
     9481 ctatattcct gatttattta actatgacga tattacgcag ttgcataacc gtttggcgtc
     9541 gatggcaccg cgcctggcgg aggatttaag cgcggtgctc aattacagcc agttgctgaa
     9601 agtgtaccgg gcgctgctga ccgaaggcgt ttccctgcgc gatatcgtca ccatcgccac
     9661 cgtgctggtc gccagtagcg cggtgaccaa agatcatatt ctgctggcag ccgatgtgcg
     9721 cctggcgctg cggcgcagca ttacccatcc gttcgttcgc aagcaggagc tgacggtgta
     9781 taccctgaat aatgagctgg aaaatctgct gactaaccta gtgaatcagg cgcaacaggg
     9841 cggtaaagtg atgctcgaca gcgtgccagt ggacccgaat atgctcaacc agttccagag
     9901 tacgatgcca caggtgaaag agcagatgaa agcggcgggg aaagacccgg tgctgctggt
     9961 gccaccgcag ctgcgccctt tgctggcgcg ttatgcaagg ttgtttgcgc cggggctgca
    10021 tgtgctgtcg tataacgaag tgccggatga gctggagttg aagattatgg gggcgttgag
    10081 ttgatggggg attcttttcc atgctttgca gcacctgaca ccattaattg acgtcacact
    10141 gcaaagagac tacgatgaca ccatacaatg acgtcatgga ttgatagtat gattaaggtc
    10201 agcgcgctag gttctcgtca tagaaaaaca ttcgacgatg taatgacgga gccgccaaaa
    10261 tctggaatta aatgggatga tgttgttgca ctcattaaag ctgtgggtgg aacaataaaa
    10321 aataacaacg gctccagacg aaaatttcag attggaacga ccaaattcag cacacatgag
    10381 ccacatccaa aaaatgtgat ggataaaggc gcagtagcag gtcttaagga gtggtttgtt
    10441 aactgtgtag gggtagatta tgaataaagc atcgaacatt atcacaatta aaggtcaacc
    10501 agccgcagta acatttgaag ccgatatcaa cgcatttcgc ggcaagttcc tcaatgtaaa
    10561 cggttattgt gactttgtag ccacaagtat tgaggcatta tatcgtgaag gcgaaagcgc
    10621 gctggatgag tggatggcgg attgtgaaga ggacggtatt gccccttttc gtgaagaaga
    10681 agagcaaaaa cgcctgacgc ttcgtgttcc tcatcacatt gattcgcggc tgaccatcgt
    10741 cgccagacaa cattccattt caaaaaacca gctcattgtt gatgtcttag aacgtgagtt
    10801 ttcagcccag atgtaaacag gtcggatgcc gcgtggcatc cgaccttttg ccagttcgtc
    10861 aatattaccc atttacaaaa tcgtctcctc cgtcaaaatc cgccgcgcgc ctaaaaagtg
    10921 atcctgccag aaaggttcag ctaactgact tacccgaatg atttccccgg tgcgcggtga
    10981 ctcaataaac tgaccgtcgc ccagatacac ccccatatgg tcggcaatct cgcggctgtg
    11041 gatatggaaa aatagcaaat ctccccggcg caggtcgtta ttcgccacaa tggttgcccg
    11101 gcgatagtga tacatctcat tagccgtgcg tgggagtttc gcctcaagga tcttgttgta
    11161 ggcataaaaa actaacccgc tacagtcgaa gccttgatcg ggccgcgtac cgccccagac
    11221 gtaaggcttg ccaagctgtt gctccagacg gtgaatcgcc acttcggtga tgttatgcag
    11281 atggtcgctg cttaagaaat ggttgttttc cgccagcgcc tgccagcgtc tgtcactcgc
    11341 cttgagcggc gcaggaaacc attctggatg ctgttttatc tgttcgcggt tgtgctggcg
    11401 tagcgccctt ctgctttcgg cattgccttc cacaatatag ctggcctgtt ttttcagatg
    11461 ggtttggtac tgttttaata aacgcgcacg attttgcatc cgctggcggg cggcgtagct
    11521 gaaagtgata tgtgatgtgg tggcgctggc aaatgaagaa aacggcaagc aaagcaacag
    11581 taatcccgac aacacaaaag agggaatcga cggcaaggac atactcaata atggctgatc
    11641 atgcattgat gttgcagtgt atcccggccc gtacctgaac aaacttaata aaccattatt
    11701 ataaaattaa taaatatatt tcagttaatt atgtaaaaac atccgtttat taaattactc
    11761 atggattgtc gctgttgctc atttgagcta caattcaagc tgaataaata tacagcacag
    11821 gagatacccc aatggctgct aacgcatttg ttcgcgcccg aatcgatgaa gatctgaaga
    11881 atcaggcggc ggacgtactg gccgggatgg ggctgaccat ctctgacctg gttcgcataa
    11941 ccctcacaaa ggtcgcgcgt gaaaaggcat tgccgtttga tttacgcgag cctaatcaat
    12001 taaccattca atcaatcaaa aacagcgaag ctggcgttga tgttcataag gccaaagacg
    12061 ccgatgattt atttgataaa ttaggaattt aaatatgatt caaagggata ttgaatactc
    12121 gggacaattt tcaaaggatg taaaacttgc acaaaagcgt cataaggata tgaataaatt
    12181 gaaatatctt atgacgcttc ttatcaataa tgctttaccg cttccagctg tttataaaga
    12241 ccacccgctg caaggttcat ggaaaggtta tcgcgatgct catgtcgaac cggactggat
    12301 cctgatttac aaacttaccg ataaactttt acgatttgag agaactggaa ctcacgcggc
    12361 gctctttggg taaagcacgc tgccgatgaa atttgtaaac cctgcgccga caggcttgcg
    12421 taaaactctt ttggtgataa tgtgagcctt ccaaatccag actattccga tttgggatct
    12481 attttgccgt cctggcgtgt gtaaccgttt tatcaaggaa taagcagtat gcgtaaaatc
    12541 gcattaattc ttgcgatgtt gttgatcccg tgcgtttcgt ttgccggttt gctgggcagc
    12601 agtagctcga ccacgccagt gagcaaagag tataagcagc agttgatggg atcccctgtc
    12661 tacatccaga tcttcaagga agaacgtacg ctcgatctct acgtcaaaat gggcgagcaa
    12721 tatcagctgc tcgacagtta taaaatctgt aaatattccg gcggcttagg gccaaaacag
    12781 cgtcagggcg atttcaaaag cccggaaggg ttttatagcg tccagcgtaa tcagttaaaa
    12841 ccagacagcc gttactacaa agcgattaat attggtttcc ccaatgccta tgaccgtgcg
    12901 catggttatg aagggaaata cctgatgatc cacggcgatt gtgtttccat cggctgctac
    12961 gcaatgacca atcagggtat tgatgagatc ttccagttcg ttactggtgc gctggtgttt
    13021 ggtcagccga gcgtgcaggt gagtatttac ccgttccgca tgaccgacgc caatatgaag
    13081 cgccataaat attccaactt taaggacttc tgggagcaac tgaagccggg ttacgactac
    13141 tttgagcaaa cccgtaagcc accgaccgtt tctgtggtca atggtcgtta cgtggtcagc
    13201 aagccgttaa gccacgaagt tgtgcagcca caactggcat caaactacac gctccccgag
    13261 gcaaaataag cgccattcgc ctggcataat cttttgccag gtttcattgc ccgtcagcgg
    13321 ctgtgttgca atcaccgtga ccacatcatt cggtgtggtc tgcgagctga agtcgatttc
    13381 cacatcctga tccagcagcg ttgccacgcc aaacggcgcg cggcgggtga tccagtgtaa
    13441 attagtcgag caatacgcca tcacatagcg cccgtcagaa agcagcatgt tgaaaacgcc
    13501 cttctgccgc agttcatccg ccagtgaggc gatatattta aacactgccg ccatgttgcc
    13561 cggtgtgcgc gggtaacgct gcgttaattt atgcaggagc cagcagaagg ctttttcgct
    13621 atcggtttca ccgaccgggc ggaagttgcc ggtttccagt gatttgtagc ccgtcagttg
    13681 tccgttatgg gcataagtcc agttacgccc ccataactcg cgggtaaatg ggtgagtatt
    13741 ttccagcgcc acctcgcccc gattagcctg gcgaatatga gccaccaccg aacaggattt
    13801 tatcgggtag tcctggacaa gtttggcgat gggggaatta aagctgggtt gtggatcttt
    13861 aaatgtgcga cagcctttac cttcgtaaaa ggtaatgccc cagccatctt tatgtggccc
    13921 ggttccacca ccacgctgta caagtccggt gaaactaaag cagatatcgg tagggacgtt
    13981 ggcgctcatc ccgagcaatt cgcacatggg taacctccac aacagcggga gcgaatggcc
    14041 cccggcgaag attgatttaa gaattttcag gtcggatgcg gcgtaaacga attattcggc
    14101 ctacggttcg ggtacagatt tgtaggcatg ataagacgcg tcagcatcgc atcaggcatc
    14161 agcgcacaag tgccggatgc ggcgtaaacg ccttatccag cctacgccag acttacttaa
    14221 ccatctcttt ttcaattaac tggatcagga tatggatcac tttaatgtga atctcctgaa
    14281 tgcggtcggc ataaccaaag tgcggtacgc gaatttcgat atccgccgtg ccagccattt
    14341 tgccgccgtc tttaccggtc agggtgatca ctttcatccc cttctcacgc gccgctgcga
    14401 tcgctttgat cacgtttgca gagttaccgg aggtggagat ccccagcagt acatcgcctt
    14461 cgcgacctac cgcttcaacg taacgggaga aaatatcatt gaaaccgaaa tcgttaccga
    14521 cgcatgaaat atgactaacg tcagaaatag caatcgccgg gtagcccgga cggttttcac
    14581 ggtagcgacc ggtcaactct tcggcaaagt gcatagcgtc gcaatgggaa ccgccgttgc
    14641 cgcaggaaag cactttgcca ccggctttaa agctgtctgc taacaggacc gccgcgcgct
    14701 gaatggcgtg aatattggcg tcatctttta aaaagttagc cagcgtttcc gccgcttcgt
    14761 tcagttcgtt acgaataaga tcctggtaca tgaggatatc cttcagcata aatgtaatag
    14821 acaaaatgca gtgtaccgga taccgccaaa agcgagaagt acgggcaggt gctatgacca
    14881 ggactttttg acctgaagtg cggataaaaa cagcaacaat gtgagctttg ttgtaattat
    14941 attgtaaaca cattgctaaa tgtttttaca tccactacaa ccatatcatc acaagtggtc
    15001 agacctccta caagtaaggg gcttttcgtt atgatgattt tgagtattct cgctacggtt
    15061 gtcctgctcg gcgcgttgtt ctatcaccgc gtgagcttat ttatcagcag tctgattttg
    15121 ctcgcctgga cagccgccct cggcgttgct ggtctgtggt cggcgtgggt actggtgcct
    15181 ctggccatta tcctcgtgcc atttaacttt gcgcctatgc gtaagtcgat gatttccgcg
    15241 ccggtatttc gcggtttccg taaggtgatg ccgccgatgt cgcgcactga gaaagaagcg
    15301 attgatgcgg gcaccacctg gtgggagggc gacttgttcc agggcaagcc ggactggaaa
    15361 aagctgcata actatccgca gccgcgcctg accgcagaag agcaagcgtt tctcgacggt
    15421 ccggtagaag aagcctgccg gatggcgaat gatttccaga tcacccatga gctggctgat
    15481 ttgccgccgg agttgtgggc gtaccttaaa gagcatcgtt tcttcgcgat gatcatcaaa
    15541 aaagagtacg gcgggctgga gttctcggct tatgcccagt ctcgcgtgct gcaaaaactc
    15601 tccggcgtga gcgggatcct ggcgattacc gtcggcgtgc cgaactcatt aggcccgggc
    15661 gagctgctgc aacattacgg cactgacgag cagaaaaatc actatctgcc gcgtctggcg
    15721 cgtggtcagg agatcccttg ctttgcactg accagcccgg aagcgggttc cgatgcaggc
    15781 gctatcccgg ataccgggat tgtctgcatg ggcgaatggc agggccagca ggtgctgggg
    15841 atgcgtctga cctggaacaa acgctacatt acgctggcac cgattgcgac cgtgcttggg
    15901 ctggcgttta aactctccga cccggagaaa ttgctgggtg gcgcagaaga tttaggtatt
    15961 acctgtgcgc tgatcccaac caccacgccg ggcgtggaaa ttggtcgtcg ccacttcccg
    16021 ctgaacgtgc cgttccagaa tggaccgacg cgcggtaaag atgtcttcgt accgatcgat
    16081 tacattatcg gcgggccgaa aatggccggg caaggctggc ggatgctggt ggagtgcctc
    16141 tcggtaggcc gcggcatcac cctgccttcc aactcaaccg gcggcgtgaa atcagtagcg
    16201 ctggcaacgg gcgcgtatgc tcacattcgc cgtcagttca aaatctctat cggtaagatg
    16261 gaagggattg aagagccgct ggcgcgtatt gccggtaatg cctacgtgat ggatgctgcg
    16321 gcatcgctga ttacctacgg cattatgctc ggcgaaaaac ctgccgtgct gtcggctatc
    16381 gttaagtatc actgtaccca ccgcgggcag cagtcgatta ttgatgcgat ggatatcact
    16441 ggtggcaaag gcattatgct cgggcagagc aacttcctgg cgcgtgctta ccagggcgca
    16501 ccgattgcca tcaccgttga aggggccaac attctgaccc gcagcatgat gatcttcgga
    16561 caaggagcga ttcgttgcca tccgtacgtg ctggaagaga tggaagcagc gaagaacaat
    16621 gacgtcaacg cgttcgataa actgttgttc aaacatatcg gtcacgtcgg tagcaacaaa
    16681 gttcgcagct tctggctggg cctgacgcgc ggtttaacca gcagcacgcc aaccggcgat
    16741 gccactaaac gctactatca gcacctgaac cgcctgagcg ccaacctcgc cctgctttct
    16801 gatgtctcga tggctgtgct gggcggcagc ctgaaacgtc gtgagcgcat ctcggcacgt
    16861 ctgggggata ttttaagcca gctttacctc gcctctgccg ttctgaagcg ttatgacgac
    16921 gaaggccgta atgaagccga cctgccgctg gtgcactggg gcgtacaaga tgcgctgtat
    16981 caggctgaac aggcgatgga tgatttactg caaaacttcc cgaaccgcgt ggttgccggg
    17041 ctgctgaatg tggtgatctt cccgaccgga cgtcattatc tggcaccttc tgacaagctg
    17101 gatcataaag tggcgaagat tttacaagtg ccgaacgcca cccgttcccg cattggtcgc
    17161 ggtcagtacc tgacgccgag cgagcataat ccggttggct tgctggaaga ggcgctggtg
    17221 gatgtgattg ccgccgaccc aattcatcag cggatctgta aagagctggg taaaaacctg
    17281 ccgtttaccc gtctggatga actggcgcac aacgcgctgg cgaaggggct gattgataaa
    17341 gatgaagccg ctattctggt gaaagctgaa gaaagccgtc tgcgcagtat taacgttgat
    17401 gactttgatc cggaagagct ggcgacgaag ccggtaaagt tgccggagaa agtgcggaaa
    17461 gttgaagccg cgtaattgaa taacggagcc gaaaggctcc gtttctttat ccgctaatta
    17521 tttaaaatta aagccatccg gatggttttc caggctgccg gtcaacgccg cgaacagcac
    17581 cgttttacca tcaatcgaaa gcgcatcgtt cacattcagc caggtgagtt tctcttgcga
    17641 cgttttctca tcaatagtcg agaacagccc cgtcatctga ttagatttct cggaccacat
    17701 cacagcgata cgttgcgagc cacagtcatg cggtttgcac gcgctcatca cctgatacgt
    17761 ctcatctccc aacgttacgg tttgtgcggg agtataagta ccgcctttca tcacccaggc
    17821 aggcagctta tgcccttgta ccatctgatt aaatgcagct ttggtggttt cgccctttgc
    17881 aaggctgcta atggttaaat catcctgcgc cattgcactg gtggcgatga ccagagcggc
    17941 gactgtcgtt attgccttaa acatcattcc tcccgagctt atcctgccca tgttattaac
    18001 cttctaaagc ctggcgataa cacgataatt tgcaaatcag atatttccaa tatcagcatt
    18061 tgttatcaat ttattctgcg ctaaggcaac tgcctgataa ggtgttaaag ttataaaacc
    18121 cttcgcataa aggagttgta caccgtgcct ggtttgaaga ttacgctttt gcagcaacca
    18181 ctggtgtgga tgaatggccc tgccaacctg cgtcattttg atcgtcaact ggaaggtatt
    18241 accgggcgcg atgtgatcgt tctaccggag atgtttacca gcggctttgc catggaagcg
    18301 gcagcttcgt cgctagcaca agatgacgta gtgaactgga tgacagctaa ggcgcagcag
    18361 tgcaatgcgc tgattgcagg cagtgttgca ttacaaacgg agtctggttc ggttaaccgc
    18421 tttttgctgg ttgagccggg cggcacggta catttttatg ataagcgtca tctgttccgc
    18481 atggcagatg agcatctaca ttataaagcg ggcaatgcgc gagtgattgt ggaatggcgc
    18541 ggctggcgta ttttgccgct ggtgtgctac gacttacgtt ttcctgtgtg gtcgcgcaat
    18601 ctcaacgatt atgacctcgc cctgtacgtc gccaactggc ctgctccgcg ctctctgcac
    18661 tggctggcat tgctgacggc ccgcgcgatt gagaatcagg cgtatgtggc gggatgcaat
    18721 cgcgtcggca gcgatggcaa cggctgccat tatcgcggtg acagccgggt gattaatcca
    18781 caaggcgaga ttatcgctac tgccgacgcg catcaggcaa cgcgcattga tgccgagctg
    18841 tcgatggcgg tgttaaggga atatcgcgat aagtttccgg catggcggga tgcggatgag
    18901 tttaggttgc ggtgagtggg ggttgtgttt aaggctgggg gagagtcggg gtaagactac
    18961 gaaagcccgc tctccgcaag gactgacgcc agatagtttc tgtccatagc tgcttttcgc
    19021 atcttacgtc ttaaccctgc cttgaatacc ttatcattcg tcaaaatatt aatagcgatg
    19081 tgccgtatcc ctgaaaataa ttctgcggcg tttcctcttc ttattttgca gtcgtcttca
    19141 ttcattacca cgtccagacg ccagtgcagc ttattctcca cgtgccagtg gtttcggatt
    19201 gctgtggcga acttttctgc ggttaaatca gcagaactga tataatatct gaccgtcatt
    19261 tctggctctt tcttttgttc tgctattatt gaccgaaagg agactgccac gcata
//
LOCUS       DAZFQD010000100        19082 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.100, whole genome shotgun sequence.
ACCESSION   DAZFQD010000100 DAZFQD010000000
VERSION     DAZFQD010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19082
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.100"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            74..1459
                     /gene="dcuC"
                     /locus_tag="ACHKTW_003497"
     CDS             74..1459
                     /gene="dcuC"
                     /locus_tag="ACHKTW_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HFO6464449.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AGAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFLRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTGK"
     gene            complement(1500..2180)
                     /gene="dpiA"
                     /locus_tag="ACHKTW_003498"
                     /gene_synonym="citB"
     CDS             complement(1500..2180)
                     /gene="dpiA"
                     /locus_tag="ACHKTW_003498"
                     /gene_synonym="citB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component response regulator DpiA"
                     /protein_id="HFO6464450.1"
                     /translation="MTAPLTLLIVEDETPLAEMHAEYIRHIPGFSQILLAGNLAQARM
                     MIERFKPGLILLDNYLPDGRGINLLHELVQAHYPGDVVFTTAASDMETVSEAVRCGVF
                     DYLIKPIAYERLGQTLTRFRQRKHMLESIDSASQKQIDEMFNAYARGEPKDELPTGID
                     PLTLNAVRKLFKEPGVQHTAETVAQALTISRTTARRYLEYCASRHLIIAEIVHGKVGR
                     PQRIYHSG"
     gene            complement(2149..3807)
                     /gene="dpiB"
                     /locus_tag="ACHKTW_003499"
     CDS             complement(2149..3807)
                     /gene="dpiB"
                     /locus_tag="ACHKTW_003499"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase DpiB"
                     /protein_id="HFO6464451.1"
                     /translation="MLQLNENKQFAFFQRLAFPLRIFLLILVFSIFVIAALAQYFTAS
                     FEDYLTLHVRDMAMNQAKIIASNDSVISAVKTRDYKRLATIANKLQRDTDFDYVVIGD
                     RHSIRLYHPNPEKIGYPMQFTKQGALEKGESYFITGKGSMGMAMRAKTPIFDDDGKVI
                     GVVSIGYLVSKIDSWRAEFLLPMAGVFVVLLGILMLLSWFLAAHIRRQMMGMEPKQIA
                     RVVRQQEALFSSVYEGLIAVDPHGYITAINRNARKMLGLSSPGRQWLGKPIAEVVRPA
                     DFFTEQIDEKRQDVVANFNGLSVIANREAIRSGDDLLGAIISFRSKDEISTLNAQLTQ
                     IKQYVESLRTLRHEHLNWMSTLNGLLQMKEYDRVLAMVQGESQAQQQLIDSLREAFAD
                     RQVAGLLFGKVQRARELGLKMIIVPGSQLSQLPPGLDSTEFAAIVGNLLDNAFEASLR
                     SDEGNKIVELFLSDEGDDVVIEVADQGCGVPESLRDKIFEQGVSTRADEPGEHGIGLY
                     LIASYVTRCGGVITLEDNDPCGTLFSIYIPKVKPNDSSINPIDR"
     gene            4187..5245
                     /gene="citC"
                     /locus_tag="ACHKTW_003500"
     CDS             4187..5245
                     /gene="citC"
                     /locus_tag="ACHKTW_003500"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008771 - [citrate (pro-3S)-lyase] ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006101 - citrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HFO6464452.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTSVPGVMVLMENSATRLKRYAESLKKFRHPGNKIGCIVMNANPFTNG
                     HRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMR
                     YWLETPTISAPPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKTPA"
     gene            5260..5556
                     /gene="citD"
                     /locus_tag="ACHKTW_003501"
     CDS             5260..5556
                     /gene="citD"
                     /locus_tag="ACHKTW_003501"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HFO6464453.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILRARLEALLARASGIPALPWEDCQ"
     gene            5553..6461
                     /gene="citE"
                     /locus_tag="ACHKTW_003502"
     CDS             5553..6461
                     /gene="citE"
                     /locus_tag="ACHKTW_003502"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006084 - acetyl-CoA metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HFO6464454.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHARRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            6472..8004
                     /gene="citF"
                     /locus_tag="ACHKTW_003503"
     CDS             6472..8004
                     /gene="citF"
                     /locus_tag="ACHKTW_003503"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008814 - citrate CoA-transferase activity
                     [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HFO6464455.1"
                     /translation="MTQKIEQSQRQERVAAWNRRAECDLAAFQNSPKQTYQAEKARDR
                     KLCANLEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVDRVGDAAKIGAGATRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDTQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            8008..8559
                     /gene="citX"
                     /locus_tag="ACHKTW_003504"
     CDS             8008..8559
                     /gene="citX"
                     /locus_tag="ACHKTW_003504"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050519 - holo-citrate lyase synthase
                     activity [Evidence IEA];
                     GO_process: GO:0051191 - prosthetic group biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HFO6464456.1"
                     /translation="MHLLPELASHHAVSIPELLVSRDERQARQHVWLKRHPVPLVSFT
                     VVAPGPIKDSEVTRRIFNHGVTALRALAAKQGWQIQEQAALVSASGPEGMLSIAAPAR
                     DLKLATIELEHSHPLGRLWDIDVLTPEGEILSRRDYSLPPRRCLLCEQSAAVCARGKT
                     HQLTDLLNRMEALLNDVDACNVN"
     gene            8534..9412
                     /gene="citG"
                     /locus_tag="ACHKTW_003505"
     CDS             8534..9412
                     /gene="citG"
                     /locus_tag="ACHKTW_003505"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HFO6464457.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSQLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMAINGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPADLDYLRQFDRECIERNLSP
                     GGSADLLILTWFLAQI"
     gene            9463..10926
                     /gene="citT"
                     /locus_tag="ACHKTW_003506"
     CDS             9463..10926
                     /gene="citT"
                     /locus_tag="ACHKTW_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
                     /protein_id="HFO6464458.1"
                     /translation="MSLAKDNIWKLLAPLVVMGVMFLIPVPDGMPPQAWHYFAVFVAM
                     IVGMILEPIPATAISFIAVTICVIGSNYLLFDAKELADPAFNAQKQALKWGLAGFSST
                     TVWLVFGAFIFALGYEVSGLGRRIALFLVKFMGKRTLTLGYAIVIIDILLAPFTPSNT
                     ARTGGTVFPVIKNLPPLFKSFPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNVL
                     GLEFVSKIAGIQISWLQWFLCFLPVGVILLIIAPWLSYVLYKPEITHSEEVATWAGDE
                     LKTMGALTRREWTLIGLVLLSLGLWVFGSEVINATAVGLLAVSLMLALHVVPWKDITR
                     YNSAWNTLVNLATLVVMANGLTRSGFIDWFAGTMSTHLEGFSPNATVIVLVLVFYFAH
                     YLFASLSAHTATMLPVILAVGKGIPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIY
                     GCGYVKSKDYWRLGAIFGVIYISMLLLVGWPILAMWN"
     gene            11040..11846
                     /gene="rna"
                     /locus_tag="ACHKTW_003507"
     CDS             11040..11846
                     /gene="rna"
                     /locus_tag="ACHKTW_003507"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HFO6464459.1"
                     /translation="MKAFWRNAALLAVSLLPFSSANALALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKADFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEARASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKTFVIDKVGY"
     gene            12076..12486
                     /gene="rnk"
                     /locus_tag="ACHKTW_003508"
     CDS             12076..12486
                     /gene="rnk"
                     /locus_tag="ACHKTW_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HFO6464460.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            12624..12713
                     /gene="yldA"
                     /locus_tag="ACHKTW_003509"
     CDS             12624..12713
                     /gene="yldA"
                     /locus_tag="ACHKTW_003509"
                     /inference="COORDINATES: protein motif:HMM:NF041476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YldA"
                     /protein_id="HFO6464461.1"
                     /translation="MAEAFYILIGFLIMAAIIVMAVLYFENHS"
     gene            complement(12717..13955)
                     /gene="ybdR"
                     /locus_tag="ACHKTW_003510"
     CDS             complement(12717..13955)
                     /gene="ybdR"
                     /locus_tag="ACHKTW_003510"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="HFO6464462.1"
                     /translation="MKALTYHGPHHVQVENVPDPGIEQADDIILRITATAICGSDLHL
                     YRGKIPQVKHGDIFGHEFMGEVVETGKDVKNLQKGDRVVIPFVIACGDCFFCRLQQYA
                     ACENTNAGKGAALNKKQIPAPAALFGYSHLYGGVPGGQAEYVRVPKGNVGPFKVPPLL
                     SDDKALFLSDILPTAWQAAKNAQIQQGSSVAVYGAGPVGLLTIACARLLGAEQIFVVD
                     HHPYRLRFAADRYGAIPINFDEDSDPAQSIIEQTAGHRGVDAVIDAVGFEAKGSTTET
                     VLTNLKLEGSSGKALRQCIAAVRRGGIVSVPGVYAGFIHGFLFGDAFDKGLTFKMGQT
                     HVHAWLGELLPLIEKGLLKPEEIVTHYMPFEEAARGYEIFEKREEECRKVILVPGAQS
                     AEAAQKAVSGLVNAMPGGTI"
     gene            14176..14604
                     /gene="uspG"
                     /locus_tag="ACHKTW_003511"
     CDS             14176..14604
                     /gene="uspG"
                     /locus_tag="ACHKTW_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HFO6464463.1"
                     /translation="MYKTIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            complement(14725..16290)
                     /gene="ahpF"
                     /locus_tag="ACHKTW_003512"
     CDS             complement(14725..16290)
                     /gene="ahpF"
                     /locus_tag="ACHKTW_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HFO6464464.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGDIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(16419..16982)
                     /gene="ahpC"
                     /locus_tag="ACHKTW_003513"
     CDS             complement(16419..16982)
                     /gene="ahpC"
                     /locus_tag="ACHKTW_003513"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HFO6464465.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            17354..18100
                     /gene="dsbG"
                     /locus_tag="ACHKTW_003514"
     CDS             17354..18100
                     /gene="dsbG"
                     /locus_tag="ACHKTW_003514"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308670.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HFO6464466.1"
                     /translation="MLKKILLLALLPAIAFAEELPSPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFADPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQEYESSGGKLKLNVPANVSTEQMKVLSANEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNIIMGNK"
     gene            18309..>19082
                     /locus_tag="ACHKTW_003515"
     CDS             18309..>19082
                     /locus_tag="ACHKTW_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO6464467.1"
                     /translation="MANLYDLKKFDLNLLVIFECIYQHLSISKAAESLYITPSAVSQS
                     LQRLRAQFNDPLFIRSGKGIAPTTTGLNLHHHLEKNLKGLEQTINIVNKSELKKNFII
                     YGPQLISCSNNSMLIRCLRQDASVEIECHDILMSAENAEELLVHRKADLVITQMPVIS
                     RSVICMPLHTIRNTLICSNKHPRITDNSTYEQIMAEEFTQLISKSAGVDDIQMEIDEK
                     FMNRKISFRGSSLLTIINSIAVTDLLGIVPYELYNSYRDF"
BASE COUNT         4612 a         4963 c         4665 g         4842 t
ORIGIN      
        1 attgggcata ttttttcctt aagctttagg aattttttat tatttacttt ggggcctgga
       61 gacaggaaaa attatgctga cattcattga actccttatt ggggttgtgg ttattgtggg
      121 tgtagctcgc tacatcatta aagggtattc cgccactggt gtgttatttg tcggtggcct
      181 gttattgctg attatcagtg ccattatggg gcacaaagtg ttaccgtcca gccaggcttc
      241 aacaggctac agcgccacgg atatcgttga atacgttaaa atattactaa tgagccgcgg
      301 cggcgacctc ggcatgatga ttatgatgct gtgtggattt gccgcttaca tgacccatat
      361 cggcgcgaat gatatggtgg tcaagctggc gtcaaaacca ttgcagtata ttaactcccc
      421 ttacctgctg atgattgccg cctattttgt cgcctgtctg atgtctctgg ccgtctcttc
      481 cgcaaccggt ctgggtgttt tgctgatggc aaccctattt ccggtgatgg taaacgttgg
      541 tatcagtcgt ggcgcaggtg ctgccatttg tgcctccccg gcggcgatta ttctcgcacc
      601 gacttcaggg gatgtggtgc tggcggcgca agcttccgaa atgtcgctga ttgacttcgc
      661 cttcaaaacg acgctgccta tctcaattgc tgcaattatc ggcatggcga tcgcccactt
      721 cttctggcaa cgttatctgg ataaaaaaga gcacatctct catgaaatgt tagatgtcag
      781 tgaaatcacc accactgctc ctgcgtttta tgccattttg ccgttcacgc cgatcatcgg
      841 tgtactgatt tttgacggta aatggggtcc gcaattacac atcatcacta ttctggtgat
      901 ttgtatgctg attgcctcca ttctggagtt cctccgcagc tttaataccc agaaagtttt
      961 ctctggtctg gaagtggctt atcgcgggat ggcagatgcg tttgctaacg tggtgatgct
     1021 gctggttgcc gctggggtat tcgctcaggg gcttagcacc atcggcttta ttcaaagtct
     1081 gatttctatc gctacctcgt ttggttcggc gagtatcatc ctgatgctgg tattggtgat
     1141 tctgaccatg ctggcggcag tcacgaccgg ttcaggcaat gcgccgtttt atgcgtttgt
     1201 tgagatgatc ccgaaactgg cgcactcttc cggcattaac ccggcgtatt tgactatccc
     1261 gatgctgcag gcgtcaaacc tgggtcgtac cctatcaccc gtttctggcg tagtcgttgc
     1321 ggttgccggg atggcgaaga tctcgccgtt tgaagtcgta aaacgcacct cggtaccggt
     1381 gcttgttggt ttggtgattg ttatcgttgc tacagagctg atggtgccag gaacggcagc
     1441 agcggtcaca ggcaagtaaa tagtaatgcc ggaggcaagt ttcctccggc tttatcatgt
     1501 cacccactgt ggtatatgcg ttgcggtctg ccaactttgc cgtgaacaat ttcagcaata
     1561 atcagatggc ggctggcgca atattcaaga taacgcctgg cagtggtgcg actgatggtc
     1621 agtgcctgcg ccaccgtttc tgccgtatgt tgcacaccag gctctttaaa cagttttcgc
     1681 accgcgttta gcgtcaaggg atcaatgccg gtcggcagtt cgtctttagg ttcaccgcga
     1741 gcataggcat taaacatctc atcaatttgc ttctggctgg cgctatcaat actttccagc
     1801 atatgtttac gctggcggaa acgggttagc gtttgcccca gccgttcata ggcaatgggc
     1861 ttaatgagat aatcaaatac accacaacgt acggcttcag acaccgtttc catatcgctg
     1921 gctgcagtgg taaacaccac gtcgccggga taatgcgcct gcaccagttc atgcaataaa
     1981 ttaatccctc tgccgtcagg aagatagtta tcgagcaaga ttagccccgg cttaaaacgc
     2041 tcgatcatca ttcgggcctg cgccaggttt cccgccagta atatctgact gaatccggga
     2101 atgtgacgaa tatattccgc atgcatctct gccagcggcg tttcgtcctc aacgatcaat
     2161 agggttaatg gagctgtcat taggtttcac tttcggaata tagattgaaa ataaggtacc
     2221 gcagggatca ttatcttcga gagtgataac accaccgcag cgcgttacgt agctggcaat
     2281 caagtacaac ccaatgccat gttcaccggg ctcgtcagca cgcgtactga ccccctgctc
     2341 aaatattttg tctcgtagag actctggaac gccgcagccc tgatcggcga cttcaatcac
     2401 cacatcatcg ccttcatcgc tgaggaataa ttcaacgatc ttgtttcctt catcgctacg
     2461 caggctggct tcgaaggcgt tatcaagtaa attgcccaca atggctgcaa actcggtgct
     2521 atccagtcct ggcggcagtt gcgaaagctg gctaccgggg acaatgatca tttttagccc
     2581 cagttcccgg gcgcgctgca ctttaccaaa aagcagcccc gccacctggc gatcggcaaa
     2641 cgcctcgcgc aggctgtcaa taagctgttg ctgggcctga gactccccct gcaccatcgc
     2701 cagcacgcga tcatactctt tcatctgcaa cagaccattg agcgtcgaca tccaattgag
     2761 atgctcgtgt cgcaatgtac gaaggctctc gacgtattgt tttatttgcg tcagttgcgc
     2821 attgagggtg gaaatttcgt ctttactacg aaagctgata atggccccca gcaaatcatc
     2881 acctgaacga atagcttccc gattggcaat aacgctcaga ccgttaaagt tagccaccac
     2941 atcctgacgt ttttcatcaa tctgttcggt aaagaaatcg gcgggcctga ccacttcagc
     3001 aatgggttta cccaaccatt gccgtccggg agagctcagc cccagcatct ttcttgcgtt
     3061 acgattgatg gcggtaatgt aaccatgcgg atccaccgca atcagccctt cataaaccga
     3121 actaaacagc gcctcttgct gacggaccac gcgtgcgatt tgctttggct ccatgcccat
     3181 catttgccga cggatatgcg ctgccaggaa ccacgacagc aacatcagaa tccctaacag
     3241 cacgacaaac acgcctgcca tcggtaataa aaactcagcc cgccagctat cgattttact
     3301 caccaggtag ccaatcgaca ccacgccgat gacttttcca tcgtcatcaa agattggcgt
     3361 tttggcgcgc atcgccatcc ccattgaccc tttcccggta atgaagtagc tctccccttt
     3421 ctccagcgcg ccctgtttgg tgaactgcat aggataacca attttctccg gattaggatg
     3481 gtaaaggcgg atcgagtgcc ggtccccaat caccacataa tcaaaatcgg tatctctttg
     3541 taatttgtta gcgatggtcg ccagccgttt gtagtcacgc gttttcaccg cagagatgac
     3601 actgtcattg gaggcaataa ttttcgcctg attcattgcc atgtcgcgta catgaagcgt
     3661 taaatagtcc tcaaaactgg ccgtaaaata ttgcgccagg gctgcaatga caaatattga
     3721 gaacaccaga atcagcaaaa agatacgcag cggaaatgcc agtctttgga aaaatgcaaa
     3781 ctgtttattc tcgttaagct gcaacattgt tttccttgcg ggttgtcccg agcctctttg
     3841 acggatgtaa ttaaaatgtt gaatcaaaat acatagatat taattatgat cgtggcaaat
     3901 tttattcatt aaatcaatta aatcacttaa acctgctggt catttcgtaa ttacattaat
     3961 taaaaaactt aaaaccatta aataaataaa aaaccactaa ctctatgtga aataaatcaa
     4021 aatttcacgc cgaaatactc cttaggatgt atagcgaaag gagaaaaaga tatacctcga
     4081 tcacccccct ttctcccaag tgaaaataaa aggttatcag tttgcaacat tgaacaacat
     4141 tcgttgcaaa tcgataacaa catgcacctt caggatacta tttattatgt tcggcaatga
     4201 tattttcacc cgcgtaaaac gttcagaaaa taaaaaaatg gcggaaatcg cccaattcct
     4261 gcatgaaaat gatttgagcg ttgacaccac agtcgaagta tttattaccg taacccgcga
     4321 tgaaaagctt atcgcgtgcg gtggaattgc cggaaatatt attaaatgcg ttgctatcag
     4381 tgaatccgtt cgcggtgaag gactggcgct gacattagcc actgaactga taaacctcgc
     4441 ctatgagcgg cacagcacgc atctgtttat ttataccaaa accgaatacg aggcgctgtt
     4501 ccgccagtgc ggtttttcca cgctgaccag cgtacccggc gtgatggtgc tgatggaaaa
     4561 cagcgccacg cggctgaaac gctatgccga atcgctgaaa aaatttcgtc atccagggaa
     4621 caagattggc tgcattgtga tgaacgccaa tcccttcacg aatggtcacc gttatctgat
     4681 tcaacaagct gcggcacagt gcgactggtt gcatctgttt ttagtcaaag aagattcttc
     4741 acgcttcccc tatgaagacc ggttggatct ggtgttaaaa ggcaccgccg atattccacg
     4801 cctgactgtg catcgcggct ccgaatacat catctcccgc gctacgttcc cttgctactt
     4861 cattaaagaa cagagcgtca ttaaccattg ttacaccgaa attgatctga agattttccg
     4921 tcagtacctc gctcccgcgc tgggtgtaac tcaccgcttt gtcggtactg aacccttttg
     4981 tcgcgttacc gcccagtaca accaggatat gcgctactgg ctggaaacgc cgactatctc
     5041 cgcaccgccc atcgaactgg ttgaaattga gcggctgcgt taccaggaga tgccgatatc
     5101 cgcttcccgg gtacgtcaac tgctggcgaa aaacgatctc acggctatcg cgccgctggt
     5161 ccctgcagtc acgctgcatt atttgcagaa cctgcttgag cactctcgcc aggacgcggc
     5221 agctcgtcaa aagacccccg catgagaaac aggtgaaaaa tgaaaataaa ccagcccgcc
     5281 gttgcaggca cccttgagtc tggggatgtg atgatacgca tcgccccact cgatacgcag
     5341 gatatcgacc tgcaaatcaa tagcagcgtt gagaaacagt ttggcgatgc aattcgcacc
     5401 accattctgg acgttctcgc ccgctacaac gtgcgcggcg tacagctgaa tgtcgatgac
     5461 aaaggcgcac tggactgcat tttacgtgca cgactggaag ccctgctggc acgcgccagc
     5521 ggtatcccgg ctctgccatg ggaggattgc caatgatttc cgcttcgctg caacaacgta
     5581 aaactcgcac ccgccgcagc atgttgtttg tgcctggtgc caatgccgcg atggtcagca
     5641 actccttcat ctacccggct gatgccctga tgtttgacct cgaagactcc gtggcattac
     5701 gtgaaaaaga caccgcccgc cgcatggttt atcacgcgct gcaacatccg ctgtatcgcg
     5761 atattgaaac cattgtgcgt gtcaacgcgc tggattccga atggggtgtt aacgacctgg
     5821 aagccgtcgt tcgcggtggt gcggacgttg tgcgtctgcc gaaaaccgat accgctcagg
     5881 atgttctgga tattgaaaaa gagatcctgc gtatcgaaaa agcctgtggt cgtgaacccg
     5941 gcagcaccgg cctgctggcg gcgattgaat ctccgctggg cattacccgc gcagtggaaa
     6001 tcgctcacgc ttccgagcgt ttgatcggta tcgccctcgg tgcagaagac tatgtgcgca
     6061 acctgcgtac agaacgctcc ccggaaggaa ctgaactgct gttcgcacgc tgttccattt
     6121 tgcaggccgc gcgctctgcg ggtattcagg cgttcgatac cgtctattcc gacgctaaca
     6181 acgaagccgg attcctgcaa gaagccgccc acatcaaaca gctgggcttt gacggcaaat
     6241 cgctgatcaa cccgcgtcag attgatctgt tgcacaacct ctacgcacca acccagaaag
     6301 aagtggatca cgcccgccgc gtcgtagaag ccgctgaagc cgccgctcgc gaaggcctcg
     6361 gcgtggtttc cctgaacggc aagatggtgg acggtccggt tatcgatcgc gcccgtctgg
     6421 tgctctctcg tgcagaactt tccggcatcc gcgaagaata aggcaatcaa aatgacgcag
     6481 aaaattgaac aatctcaacg acaagaacgg gtagcggcct ggaatcgtcg cgctgaatgc
     6541 gatcttgccg ctttccagaa ctcgccaaag caaacctacc aggctgaaaa agcgcgcgat
     6601 cgcaaactgt gcgccaacct ggaagaagcg attcgtcgct ctggtttaca ggacggcatg
     6661 acggtttcct tccatcacgc tttccgtggc ggtgacctga ccgtcaatat ggtgatggac
     6721 gtcatcgcga agatgggctt taaaaacctg accctggcgt ccagctccct gagtgattgc
     6781 catgcgccgt tggtagaaca cattcgccag ggcgtggtta cccgcattta tacctccggc
     6841 ctgcgcggtc cactggcgga agaaatctcc cgcggtctgc tggcggaacc ggtgcagatc
     6901 cactctcacg gcggtcgtgt gcatctggta cagagcggcg aactgaatat cgacgtggct
     6961 ttcctcggcg tcccgtcctg tgatgaattc ggtaatgcca acggctacac cggtaaagcc
     7021 tgctgcggct ccctcggcta tgcaatggtt gatgccgaca acgcaaaaca ggttgtgatg
     7081 cttaccgaag aactgctgcc ttatccgcat aatccggcaa gcattgagca agatcaggtt
     7141 gatttaatcg tcaaagttga tcgcgttggc gatgctgcaa aaatcggcgc tggtgctacc
     7201 cgtatgacca ctaacccgcg cgaactgctt attgcccgta gcgctgcgga tgtgattgtc
     7261 aactcaggct acttcaaaga aggtttctcc atgcaaaccg gcaccggcgg cgcatcgctg
     7321 gcggtaaccc gtttcctgga agacaaaatg cgtagccgcg atattcgcgc cgacttcgcc
     7381 ctcggtggta ttaccgcgac gatggttgat ctgcacgaaa aaggtctgat ccgcaaactg
     7441 ctggatgtac agagctttga cagccatgct gcgcaatcgc tggcccgtaa ccccaatcac
     7501 atcgaaatca gcgccaacca gtacgctaac tggggttcga aaggcgcatc ggttgatcgt
     7561 ctcgacgtgg tggtactgag cgcgctggaa attgacaccc agttcaacgt taacgtgctg
     7621 accggctctg acggcgtact gcgtggtgct tccggtggtc actgcgatac cgcgattgcc
     7681 tctgcgcttt ccatcatcgt cgcgccgctg gtacgcggtc gtattccgac tctggtggat
     7741 aacgtgctga cctgcatcac cccgggctcc agtgtcgata ttctggtcac agaccacggt
     7801 atcgcagtta acccggcacg tccggaactg gcagaacgtc tgcaggaagc gggcattaaa
     7861 gtggtttcca ttgagtggct gcgcgaacgt gcgcgtctgc tgaccggtga accacagccg
     7921 attgaattca ccgaccgcgt cgttgccgtt gtgcgttacc gcgatggctc ggtgatcgat
     7981 gttgtgcatc aggtgaagga ataagccatg cacctgcttc ctgaactcgc cagccaccat
     8041 gcggtgtcaa ttcccgagct gctcgtcagc cgggatgaaa ggcaagcacg gcaacacgtc
     8101 tggctcaagc gccatcctgt tccactggtc tcctttaccg tggttgcgcc tgggccgatt
     8161 aaagacagcg aggtcacacg ccgaattttt aatcatggcg tgacagcctt gcgtgcctta
     8221 gccgcaaaac agggctggca aattcaggag caggctgcac tggtttccgc cagcgggccg
     8281 gagggcatgt tgagcattgc cgccccggct cgcgacctca agctcgccac cattgagctt
     8341 gaacatagtc atcctctcgg gcggttatgg gatatcgatg tcctgacgcc cgaaggcgaa
     8401 attctctccc gccgcgacta ttcactgccg cctcgccgct gcctgttgtg cgagcaaagc
     8461 gcagccgtct gcgcgcgtgg aaaaacccat caactgaccg atttactcaa ccgcatggag
     8521 gcactgctga acgatgtcga tgcctgcaac gtcaactaaa accacaaagc ttgcgacgtc
     8581 attaatcgat gagtacgccc tgctgggctg gcgcgccatg ctgactgaag tcaatctgtc
     8641 accgaaacca ggcctcgtgg atcgcattaa ctgcggtgcg cacaaagata tggcgctgga
     8701 agatttccac cgcagcgcgc tggcgattca gggctggtta ccccgtttca ttgaatttgg
     8761 tgcctgtagt gcagaaatgg caccggaagc ggtactccac ggattacgcc caattggtat
     8821 ggcttgcgaa ggtgatatgt tccgcgccac tgcgggcgta aacacgcata aaggcagcat
     8881 tttttcttta gggctgctat gtgcggcaat tggtcgtttg cttcaactca accaaccggt
     8941 aacgccaaca accgtttgtt ctacggcggc aagtttctgc cgtggcctga ccgatcgaga
     9001 actgcgtacc aataattcac aactgacggc aggtcaacgg ttgtaccaac agcttggcct
     9061 taccggcgca cgcggtgaag ccgaagcggg ttatccactg gtgatcaatc acgccttgcc
     9121 gcattacctc actctgctgg atcaggggtt agatcctgaa ctggcattgc tcgatacctt
     9181 gctcctactg atggcgatca acggcgatac caacgttgca tcgcgcggtg gcgagggggg
     9241 cctgcgctgg ctacagcgcg aggcgcaaac attattgcaa aaagggggca ttcgaacccc
     9301 cgccgatctc gattatctcc ggcagttcga cagggagtgt atcgaacgaa atctcagtcc
     9361 aggcggcagt gccgacctgc tgatccttac ctggttttta gcacagattt aattatttaa
     9421 gcacttgata aatttggaaa tattaatttt cggagaaccc gtatgtcttt agcaaaagat
     9481 aatatatgga aactattggc cccactggtg gtgatgggtg tcatgtttct tatccctgtc
     9541 cccgacggta tgccaccaca ggcatggcat tacttcgctg tgtttgtggc aatgattgtc
     9601 ggcatgatcc tcgagccaat tccggcaaca gcgatcagtt ttattgcggt tactatttgc
     9661 gttattggca gtaattacct gctctttgat gccaaagaat tagctgaccc agcgtttaat
     9721 gcgcaaaaac aggcgctgaa atggggtctg gctggttttt ccagcaccac ggtatggctg
     9781 gtatttggcg catttatttt tgcattaggg tatgaagttt ccgggttagg tcgtcgcatt
     9841 gcccttttcc tggtgaaatt catgggcaaa cgcacgctga cgttgggtta tgcgattgtc
     9901 attatcgaca ttctgctggc accgtttaca ccgtccaaca ccgcgcgtac cgggggtacg
     9961 gtttttccgg tcattaaaaa cctgccgccg ctgtttaaat cattccccaa cgatccgtcc
    10021 gcgcgtcgta ttggcggcta tttgatgtgg atgatggtca ttagtaccag tctgagttcg
    10081 tccatgtttg tcaccggtgc ggcaccaaac gtgctgggtc tggagttcgt cagcaaaatt
    10141 gccggtatcc agattagctg gttgcagtgg ttcctctgct tcctgccggt tggggttatc
    10201 ttgcttatca ttgcgccgtg gctttcctac gtgctgtaca aaccggaaat cacacacagt
    10261 gaagaagtgg caacctgggc gggtgatgaa ctaaaaacca tgggtgcgct gacacgcaga
    10321 gagtggacgc tgattggcct tgtattgctc agcttaggtt tgtgggtatt tggcagtgaa
    10381 gtcattaatg ctactgcggt tggtctgctg gcagtttcgc taatgctggc tctgcacgtt
    10441 gtgccgtgga aagacattac ccgctataac agcgcatgga acacgctggt caacctggca
    10501 actctggttg tgatggctaa cggcctgact cgttctggtt ttattgactg gttcgccggt
    10561 accatgagta cgcacctgga aggattctca ccaaacgcaa cggtgattgt actggttctg
    10621 gtgttctact ttgcacacta cctgtttgcc agcctgtctg cgcacaccgc aaccatgctg
    10681 ccggttattc tggccgtcgg taaaggtatt ccgggcgtac caatggaaca actgtgtatc
    10741 ctgctggtgc tgtctatcgg tatcatgggc tgtctgacgc cgtatgcaac cggtcctggg
    10801 gtgattattt acggctgtgg ctatgtgaaa tcaaaagatt actggcgtct tggcgcaatc
    10861 ttcggggtga tttacatctc tatgttgctg ttggttggct ggccgattct cgccatgtgg
    10921 aactaatacg ttcgccaaac gctgaataaa tcatcacgcc cgccaggtaa gcccacctgg
    10981 cgggcttttt tatgatttaa tagatagtat tcagtgtatg tatgagttcc acacccatta
    11041 tgaaagcatt ctggcgtaac gccgcgttgc tcgcggtttc tctgcttccc ttctcttctg
    11101 ccaacgcctt agcgttgcag gcaaaacagt atggcgattt tgatcgctat gtcctggccc
    11161 tctcctggca aaccggattt tgccagagtc aacacgatcg aaatcgtaac gaacgagatg
    11221 aatgtcgcct gcaaaccgaa acgaccaaca aagctgattt tctgaccgta catggtctgt
    11281 ggccaggatt gcctaaatcg gttgctgccc gtggtgttga tgaacgccgc tggatgcgct
    11341 tcggttgcgc tactcgccca atcccgaatc taccagaagc gcgcgccagc cgaatgtgtt
    11401 catcgccgga aaccggatta tcactggaaa cggccgctaa actaagtgaa gtcatgccag
    11461 gagctggcgg acgttcctgc ctggaacgct acgaatatgc caaacacggt gcctgctttg
    11521 gttttgatcc ggacgcatac ttcggtacga tggtacgcct gaatcaagaa attaaagaga
    11581 gcgaagccgg aaaattcctt gcggataatt acggtaaaac agtgagccgc cgtgactttg
    11641 acgccgcctt tgccaaaagc tggggaaaag agaacgtgaa agcagttaag ctaacgtgcc
    11701 agggtaaccc tgcgtatttg actgaaattc agatctcgat caaagctgac gccatcaacg
    11761 ctccgctttc tgcaaactca ttcttgccac aaccacaccc aggcaactgt ggcaaaacct
    11821 ttgtgattga taaagtgggt tattaatcat ctgatttaca aaacacgcta attcgtcttc
    11881 cataaaccgt gacgattaag aattatacat aatgcgctgc attgaggaat tccctgaatg
    11941 tgacgcaaat cacttcaaag agtgagcaac agtcagtatc atcatgtgag ttaaaccctc
    12001 gccgcctgac ggtgagggtt ttcttttggg tatgtttatc tgcgacactc gcagtaccga
    12061 cgacatggag taaaaatgtc cagaccaact atcatcatta acgacctgga tgccgaacgc
    12121 atcgatattc tgctggagca acccgcctat gctggtttgc caatcgccga cgcgttaaac
    12181 gcagagttgg atcgcgccca aatgtgttcg ccagaagaga tgccacacga cgtggtgaca
    12241 atgaacagcc gggttaaatt ccgcaatctt agcgatggcg aagtgcgtgt gcgcacgctg
    12301 gtgtatccgg caaaaatgac cgatagcaat actcagcttt ccgttatggc tccggtaggt
    12361 gccgcactgc tggggctgcg cgttggcgat tccattcact gggaacttcc gggcggcgtt
    12421 gcaacccacc ttgaagtgct ggaactcgaa taccagccag aagctgctgg cgactacctg
    12481 ctttaatccc atctgcacaa gcgataactc gctttgccca gaggatgcac tcgcatcctc
    12541 tttacgcaaa atcagcaatt tttcctggtt ctcactgttc acttttgctg caaaattatt
    12601 ccgtttcgtt tgtgggaata accatggcag aagcatttta catcctgata ggatttctga
    12661 ttatggcggc gattatcgtc atggctgtcc tttacttcga aaaccactcc tgacgatcat
    12721 attgttcccc ccggcatcgc attcaccaga cctgaaaccg ccttctgcgc cgcctctgcg
    12781 ctttgtgcac cgggtaccag aatcacctta cggcactcct cttcacgttt ttcgaaaatc
    12841 tcatatcccc gggcggcctc ttcaaacggc atatagtggg taacaatttc ttctggtttc
    12901 agtaatcctt tctcaattaa cggtaataat tctcccagcc atgcgtgaac gtgggtctgt
    12961 cccattttaa acgtcaaccc tttatcaaag gcgtcgccaa acaggaaacc gtgaataaat
    13021 ccagcgtaga cgcccggtac gctaacaatg ccgccacgcc tgaccgccgc aatacactgg
    13081 cgcaacgctt taccgctgct gccctccagt ttcaggttag tcagcaccgt ttccgtggtg
    13141 ctgcctttcg cttcaaaacc gacggcgtct attactgcat ccacgccccg gtgacctgcc
    13201 gtttgttcaa taattgactg tgccggatcg ctatcttcat cgaaattaat cgggatcgcg
    13261 ccgtagcggt cagcggcgaa acgcaagcgg tagggatgat gatcaacaac aaaaatctgt
    13321 tccgcaccga gcaaccgtgc acaggcgatt gtcaacaatc ccactggacc ggcaccatag
    13381 actgcaacgc ttgaaccttg ttggatctgc gcattttttg ctgcctgcca tgccgttggc
    13441 agaatatcag aaaggaaaag ggctttatca tctgaaagca aaggcggtac tttaaacggc
    13501 cccacattcc ctttagggac gcggacatat tccgcctgcc caccaggaac gccgccatac
    13561 aggtgactat aaccaaacaa tgccgcggga gctggtatct gttttttatt gagcgcagcg
    13621 cctttacccg cattggtatt ttcgcaggcg gcatattgct gtaatcgaca gaaaaaacag
    13681 tcgccacaag caatgacaaa cggaattacc acccggtcgc ctttttgcaa attttttacg
    13741 tcctttccgg tttcaacgac ttcccccata aattcatgac caaaaatatc gccatgttta
    13801 acctggggta ttttgcctcg ataaagatgg aggtcagagc cacagatcgc cgttgccgta
    13861 atacgcagaa taatatcatc tgcctgttca atgcccggat cgggaacatt ttctacctga
    13921 acgtgatgtg ggccgtgata agtcaatgct ttcatgcaac ctccagagac agttcaaaaa
    13981 agagtcagtc tttaagggta gctatcacag cgggaaaacg ggcggtaccg caggttttgg
    14041 gagaattcct ggtgttcagt gagaatatat taatcacaga aaccgttcgt aaggaaaaag
    14101 aaccagacaa atatcaggga taaatattat aatcatggcg aatgatttgt tcatgattaa
    14161 cagggagaaa gggttatgta taagacaatc attatgccag ttgatgtatt tgaaatggaa
    14221 ttgagcgaca aagctgttcg ccacgctgaa ttcctcgccc aggatgacgg agttattcat
    14281 ctacttcacg tactacccgg gtcagccagc ctgagcctgc accgttttgc cgctgatgtg
    14341 cgtcgttttg aagagcatct gcaacatgaa gcagaagaac gtctgcaaac gatggtcagc
    14401 cacttcacca tcgatccttc ccgcattaaa caacatgtcc gttttggtag cgtgcgggat
    14461 gaagtcaatg agttggcaga agaactgggg gctgatgttg tagttattgg ttctcgcaac
    14521 ccatcgattt cgacccatct gttaggttct aacgcctcga gcgtgatccg ccatgccaat
    14581 ctgccggtgc tggttgtgcg ttaattgcca gaataaatat cccgccctgc ccggagccat
    14641 cccggcaggg ctttttaatg ccataaaaaa gccgccaggt ttgaccccgg cggctaagca
    14701 attgcaggtg aatcttactt cttcttatgc agttttggtg cgaatcaggt agtcaaaagc
    14761 actcagagag gctttggcac cttcgccagt ggcgatgatg atctgcttgt acggaaccgt
    14821 cgtacagtca cccgctgcga atacgccttt gacgttggtt tcgcatttcg catcaatgat
    14881 aatctcaccc atgcggttac gttcgactgc gccttccagc cagttggtgt tcggcagcag
    14941 accaatctgg acgaaaatac cggccagttc gatgttgtga atatcgccgc tgacacgatc
    15001 tcgatattcc agaccaacga ctttgctgcc gtcgcctttc acttccgtgg tttgcgcatt
    15061 cagaataatg tcgacgtttt tcaggctgcg cagtttgtcc tgcagcacct ggtcggcttt
    15121 catttctggc gcaaattcca gcagagttac gtgctcaacg atacccgcca ggtcgattgc
    15181 cgcttccacg ccggagttac ctccgccgat aaccgctacg cgtttgcctt taaacagcgg
    15241 gccgtcgcag tgcgggcagt aggtcacgcc tttggtgcga tactgatctt cgcccggaac
    15301 gttcatgttg cgccattttg caccggtcgc cacgataatg ctgcgtgctt tcagtaccgc
    15361 gccagaagct gtttcaatct ggtgtagacc accttcaacc gctgccggga tcagtttgct
    15421 ggcgctctgg ctgtcgatca catcaacgtc gtattcatca acgtgaactt tcagtgcgcc
    15481 tgccagcttc tgcccttcag tcttcggtac agaaatgtag ttttcgatat caacggtatc
    15541 gaggatctga ccaccaaaac gttcgcccat cagaccggta cggatgcctt tacgtgcgga
    15601 gtaaattgct gccgctgcac ccgccgggcc ggaaccgacg attaatacgt cataagcatc
    15661 acgcttgttc agctcttctg ccgcacgttt ttccgcgcca gtatcaattt tggcaacgat
    15721 ttcagtcaac gtcatgcggc cctgaccaaa ctctttcccg tttacgaaca ctgccggaac
    15781 gcccatcacg ttgcgatcgg tgatttcgtt ctggaaggtg ccgccgtcaa ttgcagtgtg
    15841 cttgatgcgc gggttcagta cgctcatcag gttcagcgcc tgcaccacgt ccgggcagtt
    15901 gtggcaagag agcgagtaat aggtttcgaa ttcaaaatca ccgtcaatat ggcgaatctg
    15961 ctccagcaga gactgcgctt ctttcgacgg atgaccaccg gtccacagca acgccagtac
    16021 cagcgaggtg aactcgtggc ccagcgggga gcctgcaaaa cgtggcccct ggttggaacc
    16081 tgggttggtg atcaggaaag acggcttacg caccggcaag ctgttatctt ctttaaaggt
    16141 gactttgtct gacagttctg cgatttcagc caacagttcc ttgatttctg ccgatttagc
    16201 gctgtcatcc agcgtggcaa ttaactcaac aggcttggtc aatttctcaa ggtaagcctt
    16261 gagttgagtt ttcatatttg tgtcgagcat gtttatctcc tgggctttaa acatcatcat
    16321 gcaagcggcc tctgcggcta cctggatgca gcttgcatca tggtgcgggg aagaaaatcg
    16381 ggtgccgcag cacccgatta attacgcgat tgcggaactt agattttacc aaccaggtcc
    16441 agagacggag ccagagttgc ttcaccttct ttccatttag ccgggcaaac ttcacctggg
    16501 tgagaagcta cgtactgtgc tgctttgatt ttacgcagca ggtcagacgc gtcacggcca
    16561 atgccttcag cggtaacttc gattgcctgg atgataccct gcgggtcaac aacgaaggtc
    16621 gcacggtcag ccagaccttc atcttcacgc atgttgtcga agttacgggt cagggcgcca
    16681 gtcgggtcgc cgatcatcgc atatttgatt ttagcgatgg tttcagagct gctgtgccat
    16741 gctttgtggg tgaagtgagt atcggtagat actgcgtata cgtctacgcc cagtttctgc
    16801 agttcttcgt agtggtcagc aacgtcaccc agttcggtcg ggcatacgaa agtaaagtca
    16861 gccgggtaga agaagaagac gctccagcgg ccttcggtat ctttttcggt gatttcgatg
    16921 aattcgccgt ttttgaatgc ctggttttta aaaggtttaa ttttggtgtt aatcaaggac
    16981 atctatactt cctccgtgtt ttcgatgaga tgtaaggtaa ccaatttttg ccgattcggc
    17041 taatgcgttt ccattatcaa atcgataagt tttaccttac aaccttcgta agacaacttt
    17101 gtgaactttg caccgccagc ggcgataaaa agtaaaatgg ccccctttcc aggtgaccgt
    17161 gtaatctgaa ctaccctttg gtatcttcag cgcctgcctg caggctgagg tgttgccctt
    17221 acaaatgcaa caacgacatg gattacaaca ccctcataaa caaagggcaa tcacctgatc
    17281 taagctctta cctatgacag tgataggtta tgccttttac tcgacttttg cactgactga
    17341 aaaggacaaa ttaatgttaa aaaagatact tttactggct ctgcttcctg caatcgcctt
    17401 cgcagaggaa cttccttccc cagtaaaagc gattgaaaaa cagggcatta caatcatcaa
    17461 aacgttcgat gcccccggag gaatgaaagg ttatctcgga aagtatcagg atatgggcgt
    17521 caccatctac ctgactcccg atggtaagca cgctatctct ggttacatgt acaacgagaa
    17581 aggtgaaaac ctgagtaaca cacttatcga aaaagaaatt tacgcaccag ccggacgcga
    17641 aatgtggcaa cggatggaac aatcccactg gctcctcgac ggtaaaaaag atgcgccggt
    17701 cattgtctac gtgttcgccg atccgttctg cccatattgt aaacagttct ggcagcaggc
    17761 gcgcccgtgg gtagattctg gcaaagtgca attaagaaca ttgttggttg gggttatcaa
    17821 gccagaaagc ccggcgacag cagcggcaat tcttgcctcc aaagatcccg caaaaacctg
    17881 gcaagaatat gaatcctctg gtggcaagct taagctaaac gtgcctgcaa acgtaagtac
    17941 agaacaaatg aaagtgttaa gcgccaacga gaaactcatg gacgatctgg gggcaaatgt
    18001 cacaccagcg atctattaca tgagtaagga aaatacgctg caacaggccg tggggttgcc
    18061 cgatcagaaa acgcttaata tcattatggg gaataaataa aagataattc atggatggtt
    18121 gaaatacatc cctgcttttc aaccatccta aaaaagcatg aattgctcgc tctgccctaa
    18181 gtggctatgc tattttagaa aaacttaatc ctgagttaag caaaaaaata taatcaataa
    18241 aaatatatac gcaaaaaaca ttgattaagt gaatatacca tggaagaaaa atataaccgg
    18301 agtagtgtat ggccaatctc tacgacttga aaaagttcga cttaaacctt cttgtcatat
    18361 ttgagtgtat ttaccagcat ttaagcatta gcaaagctgc tgaatcacta tatatcaccc
    18421 catcagccgt gagccagtcg ttacaacgct tacgtgcgca atttaatgac cctttattca
    18481 ttcgctcagg aaaaggtatc gcgccgacga caacagggtt gaatctgcat caccatcttg
    18541 agaaaaacct caaaggcctt gaacaaacaa ttaatatagt caataagtcc gaactaaaga
    18601 aaaactttat tatctacggc cctcagctca tttcttgctc taataatagt atgctgatcc
    18661 gctgcctacg gcaagatgcc tctgtcgaga ttgagtgcca tgatatcctc atgtcagcag
    18721 agaatgctga agaactgttg gttcaccgca aagccgatct ggtgatcacc caaatgccag
    18781 tgatcagccg ttccgtcatt tgcatgccat tgcatacaat tcgcaatact ctaatttgta
    18841 gtaataagca tccgcgaatt actgacaata gcacttatga acaaattatg gcagaagagt
    18901 tcacccaatt aatatccaaa tcagcaggag ttgatgatat tcaaatggaa atagacgaga
    18961 agtttatgaa tcgcaaaatt tcatttcgtg gttcctcttt gctgacaatc attaatagca
    19021 ttgcagttac agatttactg ggaatagtcc cctatgaact ttataattcc taccgtgact
    19081 tt
//
LOCUS       DAZFQD010000101        19053 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.101, whole genome shotgun sequence.
ACCESSION   DAZFQD010000101 DAZFQD010000000
VERSION     DAZFQD010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19053
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.101"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            250..1302
                     /gene="yhiM"
                     /locus_tag="ACHKTW_003516"
     CDS             250..1302
                     /gene="yhiM"
                     /locus_tag="ACHKTW_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 domain-containing protein"
                     /protein_id="HFO6464468.1"
                     /translation="MNIYIGWLFKLIPLIMGLICIALGGFVLESSGQSEYFVAGHVLI
                     SLAAICLALFTTAFIIISQLTRGVNTFYNTLFPIIGYAGSIITMIWGWALLAGNDVMA
                     DEFVAGHVIFGVGMIAACVSTVAASSGHFLLIPKNAAGSKSDGTPVQAYSSLIGNCLI
                     AVPVLLTLLGFIWSITLLRSADITPHYVAGHVLLGLTAICACLIGLVATIVHQTRNTF
                     STKEHWLWCYWVIFLGSITVLQGIYVLVSSDASARLAPGIILICLGMICYSIFSKVWL
                     LALVWRRTCSLANRIPMIPVFTCLFCLFLASFLAEMAQTDMGYFIPSRVLVGLGAVCF
                     TLFSIVSILEAGSAKK"
     gene            complement(1618..2820)
                     /gene="yhiN"
                     /locus_tag="ACHKTW_003517"
     CDS             complement(1618..2820)
                     /gene="yhiN"
                     /locus_tag="ACHKTW_003517"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFO6464469.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQAGRRVLLIDNGKKPGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAKDETGFTLELNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGIVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVRDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLIAAKSS"
     gene            3052..4551
                     /gene="pitA"
                     /locus_tag="ACHKTW_003518"
     CDS             3052..4551
                     /gene="pitA"
                     /locus_tag="ACHKTW_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HFO6464470.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWLSLQFL"
     gene            complement(4622..4957)
                     /gene="uspB"
                     /locus_tag="ACHKTW_003519"
     CDS             complement(4622..4957)
                     /gene="uspB"
                     /locus_tag="ACHKTW_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HFO6464471.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            5348..5782
                     /gene="uspA"
                     /locus_tag="ACHKTW_003520"
     CDS             5348..5782
                     /gene="uspA"
                     /locus_tag="ACHKTW_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HFO6464472.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            complement(5881..5934)
                     /locus_tag="ACHKTW_003521"
     CDS             complement(5881..5934)
                     /locus_tag="ACHKTW_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6464473.1"
                     /translation="MVLNVVLAGDFDCVRRS"
     gene            6100..7569
                     /gene="dtpB"
                     /locus_tag="ACHKTW_003522"
     CDS             6100..7569
                     /gene="dtpB"
                     /locus_tag="ACHKTW_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
                     /protein_id="HFO6464474.1"
                     /translation="MNTTTPMGMLQQPRPFFMIFFVELWERFGYYGVQGVLAVFFVKQ
                     LGFSQEQAFVTFGAFAALVYGLISIGGYVGDHLLGTKRTIVLGALVLAIGYFMTGMSL
                     LKPDLIFIALGTIAVGNGLFKANPASLLSKCYPPKDPRLDGAFTLFYMSINIGSLIAL
                     SLAPVIADRFGYSVTYNLCGAGLIIALLVYIACRGMVKDIGSEPDFKPMSFSKLLYVL
                     LGSVVMIFVCAWLMHNVEVANLVLIVLSIVVTIIFFRQAFKLDKTGRNKMFVAFVLML
                     EAVVFYILYAQMPTSLNFFAINNVHHEILGFSINPVSFQALNPFWVVLASPILAGIYT
                     HLGNKGKDLSMPMKFTLGMFMCSLGFLTAAAAGMWFADAQGLTSPWFIVLVYLFQSLG
                     ELFISALGLAMIAALVPQHLMGFILGMWFLTQAAAFLLGGYVATFTAVPDNITDPLET
                     LPVYTNVFGKIGLVTLGVAVVMLLMVPWLKRMIATPESH"
     gene            complement(7618..8370)
                     /gene="rsmJ"
                     /locus_tag="ACHKTW_003523"
     CDS             complement(7618..8370)
                     /gene="rsmJ"
                     /locus_tag="ACHKTW_003523"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HFO6464475.1"
                     /translation="MKICLIDETGTGDGALSVLAVRWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(8378..10420)
                     /gene="prlC"
                     /locus_tag="ACHKTW_003524"
     CDS             complement(8378..10420)
                     /gene="prlC"
                     /locus_tag="ACHKTW_003524"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HFO6464476.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTKALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPIEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAELAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELAQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFDLYDENNELRGSFYLDLYARENKRGGAWMDDCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            10623..11465
                     /gene="rlmJ"
                     /locus_tag="ACHKTW_003525"
     CDS             10623..11465
                     /gene="rlmJ"
                     /locus_tag="ACHKTW_003525"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HFO6464477.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSEFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLPWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            11537..12889
                     /gene="gorA"
                     /locus_tag="ACHKTW_003526"
     CDS             11537..12889
                     /gene="gorA"
                     /locus_tag="ACHKTW_003526"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HFO6464478.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADNILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGSEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(12943..13026)
                     /gene="dinQ"
                     /locus_tag="ACHKTW_003527"
     CDS             complement(12943..13026)
                     /gene="dinQ"
                     /locus_tag="ACHKTW_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible type I toxin DinQ"
                     /protein_id="HFO6464479.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            13054..13227
                     /locus_tag="ACHKTW_003528"
     CDS             13054..13227
                     /locus_tag="ACHKTW_003528"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6464480.1"
                     /translation="MHPLTHPLPVTAHALLLDNGILTPARASVNGTTRTSDQDFESVY
                     AHYQSENASELTG"
     gene            13535..13888
                     /gene="arsR"
                     /locus_tag="ACHKTW_003529"
     CDS             13535..13888
                     /gene="arsR"
                     /locus_tag="ACHKTW_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HFO6464481.1"
                     /translation="MSFLLPIQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQ
                     PKISRHLALLRESGLLLDRKQGKWVHYRLSPHIPAWAAKIIDEAWRCEQEKVQAIVRN
                     LARQNCSGDSKNICS"
     gene            13942..15231
                     /gene="arsB"
                     /locus_tag="ACHKTW_003530"
     CDS             13942..15231
                     /gene="arsB"
                     /locus_tag="ACHKTW_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HFO6464482.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL"
     gene            15244..15669
                     /gene="arsC"
                     /locus_tag="ACHKTW_003531"
     CDS             15244..15669
                     /gene="arsC"
                     /locus_tag="ACHKTW_003531"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HFO6464483.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIYYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQKGAFSKEDGEKVVDEAGKRLK"
     gene            complement(15792..16088)
                     /locus_tag="ACHKTW_003532"
     CDS             complement(15792..16088)
                     /locus_tag="ACHKTW_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFO6464484.1"
                     /translation="MQLEEVAKALKELGHPTRLFIFKHLVKAGEQGLPVGELQKQLGI
                     PGSTLSHHISALVSVGLVKQNRESRTLMCVSQYAMLEAIIEFLREECCVNSKIA"
     gene            16196..17197
                     /locus_tag="ACHKTW_003533"
     CDS             16196..17197
                     /locus_tag="ACHKTW_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFO6464485.1"
                     /translation="MSSWLAMLQDAAEMFVFLAVELSLLFIVISAGVSLIRQKVPDHK
                     IQQMMGARKGRGYLLAALLGAVTPFCSCSTIPMLRGLLSAKAGFGPTLTFLFVSPLLN
                     PIIVGLMWMTFGWKVTLLYAIITAGVSVLASIILDSLGFERHIIASKSSSANCCAPAK
                     TSPGTTYTPIKVSCCSPAAKAIENPVVTCCSTKAVVSINPIKLATKDALQQFKDVLPY
                     LLLGVLIGSFIYGFIPSAWIAAHAGADNPFAIPLSAVVGIPLYIRAEAVIPLASVLMT
                     KGMGLGALMALIIGSAGASLTEVILLKSMFRMPMIAAFLTVILGMAILMGYLTQLLF"
     gene            17778..18893
                     /locus_tag="ACHKTW_003534"
                     /pseudo
     CDS             17778..18893
                     /locus_tag="ACHKTW_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312403.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         4659 a         4756 c         4621 g         5017 t
ORIGIN      
        1 ctaaataatg gaaaacaatc catccctgga agataattgt aaaaaaaaca acagaataag
       61 aattaatctt aggataaata tttatttatc atggcctttt aaccggtaaa aaatggcact
      121 cggcacagta aaccccgcta tagttatgct atgcatttgt ctttaatgat atctcgtcgt
      181 tatattaaca ggctgaaatt atcattaatg cacatttcat tattagcagg gatacctgag
      241 gagtttaaag tgaacatata tatcgggtgg cttttcaaat taatcccttt gattatggga
      301 ttaatttgca tcgcgttagg tggctttgtg ctggaaagtt cagggcaaag cgagtatttc
      361 gtcgcgggtc atgtgctgat ttctctggcg gccatatgcc tggcattatt cactaccgca
      421 tttattatca tttcgcagct cacgcgcggc gttaatacgt tttacaatac attgttcccc
      481 attattggct atgcggggtc aattatcacc atgatatggg gttgggcact gttagcaggc
      541 aatgatgtga tggcagacga gtttgtcgcc ggccatgtta ttttcggcgt tggtatgatt
      601 gccgcctgtg tatcgacggt ggcagcgtca tccggtcact ttctgctcat tcccaaaaat
      661 gcagcgggga gcaagagcga tggaacgccg gtacaggctt attcttcatt aatcggtaat
      721 tgcctcattg ccgttcccgt tttactcacc ctgctcggtt tcatttggtc tattacgctg
      781 ttacgtagtg ctgacataac tccgcattat gtcgcgggtc acgtattgct tgggttaacc
      841 gcaatctgtg cctgtctaat tggccttgtt gccacaattg tccatcaaac acgtaatacg
      901 ttttcaacta aagaacactg gctgtggtgt tattgggtta tttttctcgg ctcaatcacg
      961 gtactgcagg ggatatacgt cttagtcagt tccgatgcaa gcgcccgact ggctcccggc
     1021 attattctta tttgcctcgg aatgatctgt tacagcatat tctcaaaagt ctggctactg
     1081 gcactggtat ggagacgtac ctgttcgtta gccaacagaa taccgatgat tcccgtcttc
     1141 acctgcctgt tttgcctttt cctggcatcg tttcttgcgg aaatggcgca gaccgacatg
     1201 ggatatttta ttccttcgcg ggttttggtc ggtttaggag cggtatgctt tacgttgttc
     1261 tcaatcgttt caatattaga agcaggttct gctaaaaaat aattgcaacg taccggataa
     1321 aaccagcgtt gaccatttgc gtaacgctgg tttttcttag gcatcatgaa ataacgcagc
     1381 attaatgcat agtggttaag gataaaaaaa caacaaagtt actgtttttt tcaacctgtt
     1441 catattttat aaagatctgg ccactgggtg agttttcaat cacctttcct tccaccttat
     1501 attaagcctg gagggtttca gttcacgggc tcattagaaa ataatcacaa gatagccact
     1561 atcaaaatta gctcattttt aatgcggctc taataatttt catctttagg taacaggtca
     1621 ggacgacttc gctgcaatca aatcctgcgc acaagcccac gcactcgacc acgcccactg
     1681 gaagttatag ccccccagcc agccggtaac gtccatcact tcgccgatga agtacagccc
     1741 aggcactttg cgcgcttcca tcgtccgtga agagagttcg ttggtgtcca cgccgccgag
     1801 cgtcacttcg gcagtgcgat agccttcagt gccgttgggt tgtacgcgcc agtcggtcaa
     1861 tgtgctaatc agtgcctgtt ggtcacgcac gttgagctgt tttagcgaaa catccgggat
     1921 ttgcccgagt tgctgtaagc gttcaaccaa ccgcttcggt agatgaaccg ccagtgtgtt
     1981 tttcaggctc tgattcggat gtgcgttacg ctgctcattc aggaaggttt cgagatccac
     2041 atccggtagc agattgatac tgacaaattc acccggttgc cagtagctgg aaatctgcaa
     2101 caccgccggt ccagacaagc cgcgatgggt gaagagtaag ttctcacgga aaacgatgcc
     2161 gttttcagcg gtaatcacgg aaggcaccgc cacgcccgcc agcacctgta actcttcgag
     2221 caacggttta tgcagagtga atggcaccag acccgcgcgg gtcggcagca cgttgaggcc
     2281 aaattgttcg gcaatcttat aaccaaacgg cgacgcgccc agccccggca ttgacagccc
     2341 accggtcgcg atgaccagct tttcgcaacc gacagtcatg ccgttcagtt caagcgtgaa
     2401 gcctgtttca tccttcgcca cactcagcac ttcgctacgc aatctgaagg ttacattgcc
     2461 cttctcgcac tcatccacca gcatgtcgac aatctgctgc gcggagtcat cgcagaagag
     2521 ttgtcctaac gttttctcgt gccaggcgat gccgtgttta ttgaccagat caatgaaatc
     2581 ccactgggta aaacgcgcga gtgcagactt acaaaaatgc ggattctggc tcagataagc
     2641 gcctggttcg acataaaggt tggtaaagtt gcagcgccca ccgccagaca taaggatttt
     2701 gcgccctggt tttttaccat tatcgatcag cagaacccgg cgtcctgcct gacctgccag
     2761 cgcagaacag aacatacccg ccgcaccagc gcctataata atggcatcaa acctttccac
     2821 gttgcgctcc tcttagaaaa aacgggcgtg aattgtaaag attcctcagt ggtcgcacca
     2881 gcatcaatat tactaaaagg aagtatttgc ctgaattata taagataatt attttttgag
     2941 tgaaatccat acaggaggca aatcaaaaaa agtctatatt tcactttgcc cgcgccgcga
     3001 aagtcactga taatgcgccg cgttcatgtc ctcaaaatgg cgtaacgtcc tatgctacat
     3061 ttgtttgctg gcctggattt gcataccggg ctgttattat tgcttgcact ggcttttgtg
     3121 ctgttctacg aagccatcaa tggtttccat gacacagcca acgccgtggc aaccgttatc
     3181 tatacccgcg cgatgcgttc tcagctcgcc gtggttatgg cggcggtgtt caactttttg
     3241 ggtgttttgc tgggtggtct gagtgttgcc tatgccattg tgcatatgct gccgacggat
     3301 ctgctgctta atatgggatc gtctcatggc cttgccatgg tgttctctat gttgctggcg
     3361 gcgattatct ggaacctggg tacctggtac tttggtttac ctgcatccag ctctcatacg
     3421 ctgattggcg cgatcatcgg gattggttta accaatgcgt tgatgaccgg gacgtcagtg
     3481 gtggatgcac tcaatatccc gaaagtatta agtattttcg gttctctgat cgtttcccct
     3541 attgtcggcc tggtgtttgc tggcggtctg attttcttgc tgcgtcgcta ctggagcggc
     3601 accaagaaac gcgcccgtat ccacctgacc ccagcggagc gtgaaaagaa agacggcaag
     3661 aaaaagccgc cgttctggac gcgtatcgcg ctgatccttt ccgctatcgg cgtggcgttt
     3721 tcgcacggcg cgaacgatgg tcagaaaggc attggtctgg ttatgttggt attgattggc
     3781 gtcgcgccag caggcttcgt ggtgaacatg aatgccactg gctacgaaat cacccgtacc
     3841 cgtgatgcca tcaacaacgt cgaagcttac tttgagcagc atcctgcgct gctcaaacag
     3901 gctaccggtg ctgatcagtt agtaccggct ccggaagctg gcgcaacgca acctgcggag
     3961 ttccactgcc atccgtcgaa taccattaac gcgctcaacc gcctgaaagg tatgttgacc
     4021 accgatgtgg aaagctacga caagctgtcg cttgatcaac gtagccagat gcgccgcatt
     4081 atgctgtgcg tttctgacac tatcgacaaa gtggtgaaga tgcctggcgt gagtgctgac
     4141 gatcagcgcc tgttgaagaa actgaagtct gacatgctta gcaccatcga gtatgcaccg
     4201 gtgtggatca tcatggcggt cgcgctggcg ttaggtatcg gtacgatgat tggctggcgc
     4261 cgtgtggcaa cgactatcgg tgagaaaatc ggtaagaaag gcatgaccta cgctcagggg
     4321 atgtctgccc agatgacggc ggcagtgtct atcggcctgg cgagttatac cgggatgccg
     4381 gtttccacta ctcacgtact ctcctcttct gtcgcgggga cgatggtggt agatggtggc
     4441 ggcttacagc gtaaaaccgt gaccagcatt ctgatggcct gggtgtttac ccttccggct
     4501 gcggtactgc tttccggcgg gctgtactgg ctctccttgc agttcctgta atcgtacaca
     4561 ccaaaacgag cgggtcagtt tactggcccg ctttttttat gcgttattat tgctgaagtg
     4621 cttaatgcca aatcatcaat gcaatcaggc tgaccaccac cagaccacac aatgcgctgg
     4681 tcagaataaa ctgccgacgc acccgctcac agcggcgaat aaattcatca tcgtgatgat
     4741 cgcgataacg ttgggcatag atataccaaa cgagacgcac ctgtttgttg ggttggccat
     4801 gtgaggtaaa aaagccccct ccatcaacat attgatagag caatggatcg cagttacgca
     4861 gtaccactaa caacgcgcgt agtgatgaga aatagcgcgc catgttaaca atgcaaacga
     4921 cacataaagc ccaaaataat gcgacggtgc ttatcatacc tcctccccgg cgacctgccc
     4981 gcggagttcc accccggggc taccgctccc gatacgctgc caatcagtta acaccaggtc
     5041 ctggagaaac cgcttttgtg gtgaccaaca tccgagcggc tctatagata gtgtaggaga
     5101 tcaggttgtt ttttttccag aaggttaacc actatcaata tattcatgtc gaaaatttgt
     5161 ttatctaacg ggtaagcaag gcggattgac ggatcatccg ggtcgctata aggtaaggat
     5221 ggtcttaaca ctgaatcttt acggctgggt tagccccgcg cacgtagttc gcaggacgcg
     5281 ggtgacgtaa cggcacaaga aacgctagct ggccagtcat cgacaacttt atggaaggag
     5341 taacactatg gcttataaac acattctcat cgcagtcgat ctctccccgg aaagcaaagt
     5401 tctggtagag aaagcagtct ctatggctcg cccctacaat gcgaaagttt ctctgatcca
     5461 cgtagatgta aactactctg acctatacac cgggcttatt gatgtgaatc tgggtgatat
     5521 gcagaaacgc atctctgaag agacacatca tgcgctgacc gagctttcca ctaatgcagg
     5581 ctacccaatc actgaaaccc tgagcggcag cggcgacctg ggccaggttc tggtcgatgc
     5641 aatcaagaaa tacgatatgg atctggtggt ttgtggtcac caccaggact tctggagcaa
     5701 actgatgtct tccgcacgtc agctgatcaa caccgttcac gttgatatgc tgattgttcc
     5761 gctgcgcgac gaagaagaat aatcatccct ccacgacgtg ttcctgaacg cccgcatatg
     5821 cgggcgtttt gctttttggc gcgccttgtt acctgatgca gcgtaaaccc cttatctggc
     5881 ctacgatctg cgtacgcaat caaaatctcc tgccaataca acatttaaca ccatcatatt
     5941 ttccatcatt agtgtgatca gctggttatt ttctgttgta atagtgtatt aatctattca
     6001 ccgcatcaat attaagaatc tctgacagat gtaaactttt tcgcgcgtta tcccttacgc
     6061 gttcatactt ttcaggatgg tattggaagg ttaataaata tgaatacaac aacacccatg
     6121 gggatgctgc agcaacctcg cccatttttc atgatctttt ttgtcgagtt atgggagcga
     6181 ttcggctact acggcgtgca gggcgtactg gcggttttct tcgttaaaca gcttggattc
     6241 tcgcaagagc aggcttttgt cacttttggt gcttttgctg cgctggtcta tggcctcatt
     6301 tccattggcg gctatgtcgg cgaccacctg ctggggacca aacgcaccat tgttctcgga
     6361 gcacttgtgc tggcgattgg ctacttcatg accggcatgt cgctacttaa gcctgacctg
     6421 attttcatcg ccctggggac tatcgctgtc ggtaacggcc tgtttaaagc taacccagcc
     6481 agcttgcttt cgaagtgcta tccgccgaaa gatccgcggc ttgatggcgc attcaccctg
     6541 ttctatatgt cgatcaatat cggctcgttg atagcgttat cgctggcccc tgtgatcgct
     6601 gatagattcg gttattcagt cacctacaac ctgtgcgggg cggggttaat tatcgcatta
     6661 ctggtttaca tcgcctgtcg tggaatggtg aaagacattg gttctgaacc cgacttcaag
     6721 ccgatgagct tcagcaaact gttgtacgta ttacttggca gcgtggtgat gatcttcgta
     6781 tgtgcatggc tgatgcacaa cgtagaagtc gccaatctgg tgctgattgt tctctccatc
     6841 gtcgtcacca tcatcttctt tcgtcaggca ttcaagctgg ataaaaccgg gcgcaataaa
     6901 atgtttgtcg cctttgtcct gatgctcgaa gcggtggtgt tttacattct ctacgcccag
     6961 atgccaacat cgctgaactt ctttgccatc aacaacgtgc atcatgaaat tctcggtttt
     7021 tccatcaacc cggtcagctt ccaggcgctt aacccgttct gggtggtact cgccagccca
     7081 atactggcag gcatttacac gcatctgggt aacaaaggca aagacctctc gatgccgatg
     7141 aaatttactc tcggcatgtt tatgtgctct ctgggctttt tgacggctgc agctgcggga
     7201 atgtggtttg cggatgccca ggggctgaca tcgccatggt ttatcgtgct ggtgtactta
     7261 ttccagagct taggtgaact gtttattagc gcccttggcc tggcgatgat tgctgccctg
     7321 gtgccgcagc atttgatggg ctttattctc gggatgtggt tcctgacgca ggctgccgcg
     7381 ttcttgctgg gcggctatgt ggcaacattt accgcggtgc cggacaacat taccgatccg
     7441 cttgagacgt tgcccgtcta taccaacgtg tttggtaaga ttggtctggt cacactgggc
     7501 gttgcagtag tgatgctgtt gatggtgccg tggctgaaac gcatgattgc gacgccggaa
     7561 agccattaat tattcttgca gaaagcaggg tagcgttatc gctaccctgg tttactttta
     7621 cactggcgta cctgcataaa tatcaaacct atgcccttta gtgactaccg cgtttggcgt
     7681 ggcgacattc gccagcggtg gcgcgtagtc cgggcgcttt accaccacgc gtttggtcgc
     7741 cagcaagcgt gcaggctcca gtaatccatc ggcatcaaga tccggtccca ccagcgactg
     7801 aaagacacgc atctcttttt tcactagcgc gcttttctgc ttatgcggga acatcgggtc
     7861 gagataaacc acctgcgggc gcggggtaat atcagtcagc gccgtcaggc tggaggcgtg
     7921 aattaactgc aaccgctcct gcaaccagcc gccgatttcc gcatccgcat aaccacgcgc
     7981 caggccgtcg tcgagcaatg cggcaacgac tggattacgc tccagcatcc gcacgcggca
     8041 gccgactgaa gccagcacaa aggcatcgcg ccccagccct gcggtggcat ccaccacatc
     8101 cggcaaataa tcgcctttaa tgcccaccgc tttcgccacc gcctcaccgc gaccaccgcc
     8161 gaatttgcgt cggtgcgcca tcgctccgcc aacaaaatca acaaagatgc cgccaagttt
     8221 tggctcatca cgcttgcgca attccagatg ttccggcgtt aacaccagcg ccatcaggtt
     8281 gtcttcatcg tgctccagcc cccagcggac cgccagaaca gataaggcac cgtctccggt
     8341 gcctgtttca tcaattaagc agattttcac tgaatgatca gcccttaatg ccgtaatgct
     8401 ccagcatcgc atccagctgc ggttcacgac cacggaagcg tttgaacaga tccatcggct
     8461 cttctgaacc gccacggctc agaatgttgt cgaggaacga ctgcccggtt tcacggttga
     8521 aaatgccctc ttcctcaaag cgcgagaaag catctgccgc cagtacgtca gcccacaggt
     8581 agctgtagta acctgcggca taaccaccgg cgaaaatatg gctgaaagcg tgcgggaaac
     8641 ggccccagga tggagacggt actacggcaa ccagtttctt gatttctgcc agagtttcga
     8701 ggattttcgc tccctgatcc gggcggaact cggcatggag gcggaaatcg aacaggccga
     8761 actccagctg gcgcagaata aacagcgccg cctggtagtt cttcgccgcc agcattttat
     8821 ccagcaactc tttcggcagc ggttcgccgg tttcgtagtg accggagata aacgccagcg
     8881 cctccggctc ccagcaccag ttttccataa actgactcgg cagttcgacc gcatcccacg
     8941 gcacaccgct gataccggaa acaccagcgg tttcgatgcg ggtcagcata tggtgcaggc
     9001 cgtgaccgaa ctcgtggaac agggtgatca cttcgtcgtg agtgaacagc gccggtttac
     9061 catttaccgg gcggttgaag ttacaagtca aatacgcgac cggtttttgc agcgaaccat
     9121 cagctttacg catctggcct acgcagtcat ccatccacgc cccgccgcgc ttgttttcac
     9181 gggcatacag atcgaggtag aagctgccgc gcagttcgtt attttcgtca tacagatcga
     9241 agaaacgtac atccggatgc cagacatcaa catctttacg ctctttagcg gtgatgccgt
     9301 aaatacgttt caccacttca aacaggccgt taaccgcttt gttttctggg aagtacggac
     9361 gcagctgctc gtcgctgatg ctgtagaggt gctgtttttg tttttcgctg tagtacgcga
     9421 tatcccacgg ctgcaactca tcgacgccaa attccgcttt ggcgaaggcg cgcagttgtg
     9481 ccagctcttt ttcgccttgc ggacgcgcgc gttttgccag atcggttaag aaatccagca
     9541 cctgctgcgg gttttctgcc attttagtgg caagggattt aaaggcgtag ttttcaaagc
     9601 ccagcagttg cgccagttcg tgacgcagag cgaggatctc ttccatcacc ttgctgttat
     9661 cccatttacc ggcgttcggg ccttgatcgg aggcgcgggt gctgtaagcg cgatacatct
     9721 cttcacgcaa ggcctggttg tcgcagtagg tcattaccgg caggtagctt gggatatcca
     9781 gcgtcagcag gtagccttcc agctctttcg cttcggcctg ggcttttgcc gcagccagcg
     9841 cgctttcagg catccccgcc agctccgctt cgtcggtaac gagtttggtc cagcccatcg
     9901 tcgcatcgag gacgttgttg ctgtactggt tgcccagctc ggaaagacga gtggcaatct
     9961 caccgtagcg ttgttgtttc tctattggca gaccgatgcc agagagttcg aagtcgcgca
    10021 gtgcgttatc aaccgctttt ttctgagccg tgttcagcgt ggcgtaatga tcgccatcgc
    10081 gcaggtcgcg atacgcttta tacagccctt catgttgccc tacccaggtg ctgtattccg
    10141 acagcagcgg cagggtttgt tcgtacgctt cacgcagttc cgggctattt ttcaccgagt
    10201 tcaggtggct gaccggggag aagatacgcc ccagcacatc gtccacttcc gccaacggct
    10261 ggcagagatt ttcccaggtg tacggtgctc cttgcgctac tacgcgctcc acattttcgc
    10321 ggcagtcgtt cagcgcctta gtcacggctg gaacgacatg ttccgggaga attttagaaa
    10381 acggaggcaa ttcaaaggga gtcagtaacg gattcgtcat tcgcgcagtc ctggttaaag
    10441 aggttaagga agcgctcaac aggcgcttta cataatgtgt gtagcatggg gttaagtgta
    10501 gtgaatttca atgagaaacg ttacgctttc gcggcggcgg cctcttttcg gtatactgtc
    10561 ctgatacgct tttgtgcgcc ccgaatacgg gccgattttt acttaccgga acacctttac
    10621 ccatgctcag ttatcgccac agctttcacg ctggcaacca cgccgacgtc cttaaacata
    10681 ccgttcagag cctgatcatc gagtcgctga aagagaaaga taaaccgttt ctctatctcg
    10741 acacccacgc aggggccggg cgttatcagt taggcagcga acatgccgag cgtaccggcg
    10801 aatatctcga aggcatcgcc cgtatctggc agcaggacga tttgcccgca gaactggagg
    10861 cgtacatcaa tgtggtaaaa cacttcaacc gtagcggtca gttgcgttac taccccggtt
    10921 cgccgttgat tgctcgccag ctactgcgtg aacaggacag cctgcaactg accgaactgc
    10981 acccgagcga ttacccgttg ttgcgttctg aatttcagaa agatagccgt gcgcgtgtcg
    11041 aaaaagccga cggtttccag cagcttaagg ccaaactgcc gccggtttcc cgccgtggtt
    11101 taatccttat cgacccgccg tatgaaatga aaactgacta tcaagcggtg gtcagcggga
    11161 tagcagaagg ttacaaacgt ttcgccactg gtacttacgc actgtggtat ccggtggtgc
    11221 tgcgtcagca aattaagcgc atgatccacg atctggaagc gaccggtatt cgcaaaattc
    11281 tgcaaattga actggcggta ctgccagaca gcgatcgccg tggcatgacc gcttccggca
    11341 tgattgtgat taacccgccg tggaaactgg aacagcagat gaataacgtg ctgccgtggc
    11401 tgcacagcaa actggttccg gcaggcaccg ggcacgccac cgtaagctgg atcgtgccgg
    11461 agtaattgca gccattgctg gcacctatta cgtctcgcgc tacaatcgcg gtaatcaacg
    11521 ataaggacac tttgtcatga ctaaacacta tgattacatc gccatcggcg gcggcagcgg
    11581 cggtatcgcc tccatcaacc gcgcggctat gtacggccag aaatgtgcgc tgattgaagc
    11641 caaagagctg ggcggcacct gcgtaaatgt tggctgtgtg ccgaaaaaag tgatgtggca
    11701 cgcggcgcaa atccgtgaag cgatccatat gtacggcccg gattatggtt ttgataccac
    11761 tatcaataaa ttcaactggg aaacgttgat cgccagccgt accgcctata tcgaccgtat
    11821 tcatacttcc tatgaaaacg tgctcggtaa aaataacgtt gatgtaatca aaggctttgc
    11881 ccgcttcgtt gatgccaaaa cgctggaggt aaacggcgaa accatcacgg ccgataatat
    11941 tctgatcgcc acaggcggtc gtccgagcca cccggatatt ccgggcgtgg aatacggtat
    12001 tgattctgat ggcttcttcg cccttcctgc tttgccagag cgcgtggcgg ttgttggcgc
    12061 gggttacatc gccgttgagc tggcgggcgt gattaacggc ctcggcgcga aaacgcatct
    12121 gtttgtgcgt aaacatgcgc cgctgcgcag cttcgacccg atgatttccg aaacgctggt
    12181 cgaagtgatg aacgccgaag gcccgcagct gcacaccaac gccatcccga aagcggtagt
    12241 gaaaaatgcc gatggtagcc tgacgctgga gctggaagat ggtcgcagtg aaacggtgga
    12301 ttgcctgatt tgggcgattg gtcgcgagcc tgccaatgac aacatcaacc tggaagccgc
    12361 tggcgttaaa actaacgaaa aaggctatat cgtcgtcgat aaatatcaaa acaccaatat
    12421 tgaaggtatt tacgcggtgg gcgataacac gggtgcagtg gagctgacac cggtggcagt
    12481 tgcagcgggt cgccgtctct ctgaacgcct gtttaataac aagccggatg agcatctgga
    12541 ttacagcaac attccgaccg tggtcttcag ccatccgccg attggtactg ttggtttaac
    12601 ggaaccgcag gcgcgcgagc agtatggcga cgatcaggtg aaagtgtata aatcctcttt
    12661 caccgcgatg tataccgccg tcaccactca ccgccagccg tgccgcatga agctggtgtg
    12721 cgttggatcg gaagagaaga ttgtcggtat tcacggcatt ggctttggta tggacgaaat
    12781 gttgcagggc ttcgcggtgg cgctgaagat gggtgcaacc aaaaaagact tcgacaatac
    12841 cgtcgccatt cacccaacgg cggcagaaga gttcgtgaca atgcgttaaa tgttaaaggg
    12901 ctaagagtag tgtgctctta gcccttaatt acgtttccgc tatcagttca gaagctgaag
    12961 cagaaagcgg atcagttcca gcagcgcaat taacgcccca agaacgatga ttgctttatc
    13021 aatcacccgt tttctccatg cgatggagtg agaatgcatc cgcttactca tccactgcct
    13081 gtcacggcac atgccctatt gttagataat ggcattctca ctccggccag agcatcagtt
    13141 aacggcacca cccgtacttc tgaccaggac tttgaaagcg tttatgcgca ttaccagagt
    13201 gaaaatgcct cagagctaac tggataatca tacagtacat tcaggttata aaaccagcat
    13261 gtccttgcaa tagtttcagt atggtattag cattgatgcg ttagataatg gcattctcac
    13321 tccagccagt gccaccaact cagggctgga aagtaaaaaa ccgacgcaaa gtcggttttt
    13381 ttacgtcctg attcagacct cctttcaaat gaatagccaa ctcaaaattc acacctatta
    13441 ccttcctctg cacttacaca ttcgttaagt catatatgtt tttgacttat ccgcttcgaa
    13501 gagagacact acctgcaaca atcaggagcg caatatgtca tttctgttac ccatccaatt
    13561 gttcaaaatt cttgctgatg aaacccgtct gggcatcgtt ttactgctca gcgaactggg
    13621 agagttatgc gtctgcgatc tctgcactgc tctcgaccag tcgcagccca agatctcccg
    13681 ccacctggca ttgctgcgtg aaagcgggct attgctggac cgcaagcaag gtaagtgggt
    13741 tcattaccgc ttatcaccgc atattccagc atgggcggcg aaaattattg atgaggcctg
    13801 gcgatgtgaa caggaaaagg ttcaggcgat tgtccgcaac ctggctcgac aaaactgttc
    13861 cggggacagt aagaacattt gcagttaaaa atttagctaa acacatatga attttcagat
    13921 gtgttttatc cgggaggcat tatgttactg gcaggcgcta tctttgtcct gaccatcgta
    13981 ttggttatct ggcagccgaa aggtttaggc atcggctgga gtgcaacgct cggcgcagta
    14041 ctggcgttag ttacgggcgt ggtccatccg ggtgatattc cggtggtgtg gaatatcgtc
    14101 tggaacgcga cggctgcgtt tatcgccgtc attatcatca gcctgctgct ggatgagtcc
    14161 ggcttttttg aatgggcggc gctgcacgtc tcacgctggg gtaatggtcg tggtcgcttg
    14221 ctgtttacct ggattgtcct gctcggtgct gccgttgccg ccctgtttgc caatgatggc
    14281 gcggcgctta ttttgacacc gattgtcatc gccatgctgc tggctttagg gttcagtaaa
    14341 ggcactacgc tggcgttcgt gatggcggcc ggattcattg ccgataccgc cagcctgccg
    14401 cttattgtct ccaacctggt gaatatcgtt tccgctgatt tctttggcct cggctttcgc
    14461 gaatacgcct cggtgatggt gccggtggat atcgccgcga ttgttgccac gctggtgatg
    14521 ttacatctct attttcgcaa agatattccg cagaactacg atatggcgct gctgaaatct
    14581 cccgcagaag cgatcaaaga tcctgctacg ttcaaaactg gctgggttgt tttactgctt
    14641 ctgctggtgg gatttttcgt cctggaaccg ctcggcattc cggtgagcgc cattgcagct
    14701 gtgggcgcgc tgatattatt tgtcgtcgct aaacgcggtc atgcgattaa tacgggtaaa
    14761 gtcctgcgcg gtgccccctg gcagattgtc atcttctcgc tcggcatgta tctggtggtt
    14821 tatggcctgc gcaatgccgg attaacggaa tatctttctg gcgtactcaa cgtgctggcg
    14881 gataacggcc tgtgggccgc gacgctcggc accggattcc tcaccgcctt cctctcttct
    14941 attatgaaca atatgccgac ggtactggtt ggcgcgttgt ccattgatgg cagcacggca
    15001 tctggcgtta tcaaagaagc gatggtttat gccaatgtga ttggctgcga tttgggaccg
    15061 aaaattaccc caattggtag cctggctacg ctactctggc tgcacgtact ttcgcagaag
    15121 aatatgacta tcagctgggg atattacttc cgtacaggga ttatcatgac cctgccggtg
    15181 ctttttgtga cgctggctgc gctggcgcta cgtctctctt tcactttgta atgagatatt
    15241 gatatgagca acattaccat ttatcacaac ccggcctgcg gtacgtcgcg taatacgctg
    15301 gagatgatcc gcaacagcgg tacagaaccg accattatct attatctgga aacaccgcca
    15361 acgcgcgatg agctggtcaa actcattgcc gatatgggga tttccgtacg cgcgctgttg
    15421 cgtaaaaacg tcgagccgta tgaggaactg ggccttgcag aagataaatt tactgacgat
    15481 cggttaatcg actttatgct tcagcacccg attctgatta atcgcccgat tgtggtgacg
    15541 ccgctgggaa ctcgcctgtg ccgcccttca gaagtggtgc tggaaattct gccagatgcg
    15601 caaaaaggcg cattctccaa ggaagatggc gagaaagtgg ttgatgaagc gggtaagcgg
    15661 ttgaaataaa gcggcgatat ccactgccca tagttggtaa agtgcgcttt gtttatgtcg
    15721 gatgcgacgc taacgcgtct tatccgacct acaaaaccgt gcatggcatg aaatctgtag
    15781 cgcgtagcgc atcaggcaat tttgctattt acgcagcact cctcgcgtaa gaattcaatt
    15841 atcgcttcca gcattgcgta ttgcgaaaca cacatcagcg tccgactctc acggttttgt
    15901 ttaaccagcc ctaccgatac cagtgctgaa atatggtgac tgagtgtaga gccgggtatc
    15961 ccaagctgct tttgcaactc accgaccggc aaaccttgct cccccgcttt gaccaggtgt
    16021 ttgaatatga acaaacgggt cgggtgacct agctctttca gcgctttagc aacttcttcc
    16081 aactgcatat gacaccccct ttgtctggtt gatatttcga taataccaga aatatcttga
    16141 tctgaatttc atttcgatta ttctggaaat atagatttat ccaatgaggt tacgtatgag
    16201 tagttggctt gcgatgctgc aagatgccgc agagatgttt gtgtttctcg ccgtcgagct
    16261 ttcgttgctg tttatagtga ttagtgccgg tgtcagcctg ataagacaaa aggtgccaga
    16321 ccataaaatc cagcagatga tgggggccag aaaagggaga ggctatctcc tggctgctct
    16381 gttgggagcc gttaccccgt tctgtagttg ctcgacaata cccatgttac gtggattgtt
    16441 gtcagcgaaa gccgggtttg gtccgactct cactttttta tttgtttcac cattacttaa
    16501 tcccattatc gtcggactaa tgtggatgac ctttggttgg aaagtcacct tgttgtacgc
    16561 gattatcacc gccggcgtct ccgttcttgc cagtattatc ctggattctc tggggtttga
    16621 acgtcatatc attgccagta aaagctcatc agcaaattgt tgtgctccag caaaaacttc
    16681 gccgggaacg acatatacgc ccataaaagt gagttgttgt agtccagcgg ctaaagccat
    16741 tgagaacccc gtagttacct gttgcagtac caaagctgta gtaagtatta atcccataaa
    16801 actagccacc aaagatgcgt tgcaacagtt taaagatgta ctgccatatc ttttgttagg
    16861 agtattaata ggatctttta tttatggctt tattccctca gcgtggattg ccgctcatgc
    16921 aggagcagat aatcccttcg ccatcccatt aagcgccgtt gtcggtattc cgctgtatat
    16981 ccgggctgag gcagttatcc ctctggcatc tgttctgatg acaaaaggaa tgggtctggg
    17041 agcattaatg gcattaatca tcggcagtgc cggcgcaagc ctgacggaag tgatattact
    17101 taaatcaatg ttcagaatgc cgatgatagc tgcattcctg acggttatat taggtatggc
    17161 tatcttgatg ggctatttga ctcaattgct attttaaata gatgccagca gtaaactata
    17221 agatgcatag ttagtgccat atccttctaa gtagaaaatc atagacagga taatccttaa
    17281 aaatcacaaa tgatttaact aatgcattga gaatgatgac ggctcggtca acaagtcatc
    17341 aacagacaat aaccacgtaa tcatgcttct ctgacatttt atcggagaag caatgactct
    17401 ttgtctcagt accctcctcc tttttaaaag gcagatttca gtcgctaata caacacattt
    17461 ttaaatacac tttaacctgg ttcattatta cacatctaaa acatcacaaa aatattactt
    17521 cacacactat cggagaacat aataactttt taaaatgagc gctttttgat agttgttaac
    17581 gttttccttt acgatactat cactgccttt tttttataca caaatattac cgggtggaaa
    17641 tgaaagggaa tttaaaggta attatatcag gaagatataa cgcattatat ttattgtgtg
    17701 tagaagaaaa tccattcctg ttctaattat tcaatttaaa aaccttagca taaaacagat
    17761 gatgaataag gaatgttatg tcaattgact ttaccccagg tataataaat acatatcacg
    17821 gcgatattta taactgcaca acaaataccg ataatgttaa aacgccagac acaccgaagt
    17881 ggccttgtga taattgggaa gaacaacaac ccattaattc catatactct ggagaaggat
    17941 acaaccctga acaatttgat ttagcgcaac aacaactcca acaaattaat gcatgccaca
    18001 cgaacaccac atatacaaat gcagactact caaaagttgt ggttcaactt gtaagtctta
    18061 ttaataacat tgaaacgata agttcgacac cacttacgca acagacacaa tcaatactta
    18121 atcagattaa taacattaga tatgaaaaaa acaaaagtgc tgaatgtcga atcattgttg
    18181 tcgctaatcc taaacccgac aaggcaatca taactaaaat tagcgtagaa gagggaatac
    18241 ccataacatt cagtgtccaa accatgtttt cagatactaa ttttattgct gaacaacgag
    18301 ctgacttacc aactaatata aaagatattc aaagcctgta tcaaaaaatg acgaagttat
    18361 atattgagca cagtgaaaat aaaaacagga tgaaagtctt tgccggaacc aactttattg
    18421 atttcaatat gacaggacag aacctgtccg ggttcgttct aaccttgtca aggttttatt
    18481 tcgaagatct acttaatata aatttcactg acgcaaattt aggggacact attttttaca
    18541 taaagaacac cctaccccca aattatataa agatggacaa tatcttgaca aacaaatcga
    18601 aggtttattt tcaacattat taaccattaa tgacaacttt ttacgagcaa aagcggaaat
    18661 tgcttcgaca ataattaaat ttttagaggc taggataacc aatctttcat acaatgacat
    18721 attaacatat cagcaagaat tccaaaaaca atgttataag caagttaaag cctttgcgac
    18781 actctctcga tacaataaaa ttcaaacatg ggcagaaatg tctgaatatc agttcgaagt
    18841 atttcaatac gagacgctta accccaaaaa aatgtcacac acgccttatt taaagaggcc
    18901 acttcctaat gaaaaagata taaattatgg ggtggaaata gaaataccat ctggcaaaag
    18961 aattcgctta tcaaatcatt atcagaatat aataccataa tattgattat tagcacttta
    19021 taattattga ataaaaatag attttatgta att
//
LOCUS       DAZFQD010000102        18933 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.102, whole genome shotgun sequence.
ACCESSION   DAZFQD010000102 DAZFQD010000000
VERSION     DAZFQD010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18933
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.102"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..241
                     /locus_tag="ACHKTW_003535"
     CDS             <1..241
                     /locus_tag="ACHKTW_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3289 family protein"
                     /protein_id="HFO6464486.1"
                     /translation="ISAQKISLLSFQKYAIGWSATIHFVAQDHFGLDVTDIKNKTYSK
                     YRFFRIWFFLQRHKDFAFKPFFTNFNTIERIENYL"
     gene            238..717
                     /locus_tag="ACHKTW_003536"
     CDS             238..717
                     /locus_tag="ACHKTW_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001688100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF943 family protein"
                     /protein_id="HFO6464487.1"
                     /translation="MKSKLQKIILCLFLLCCIYNLWTLRPVQILYTYSDAGNSVFLVV
                     DHLPWTDSDKINWYLKHQNEIKNQHPLPEGSWHTWYVIDIGNGFTDYKKYIEGPYEDL
                     YCFPTIKSNDNCIVKNYLMVINEYPYRNTHIGINDFTEYQLTQENKIERVFNPHDFK"
     gene            complement(771..1721)
                     /gene="corA"
                     /locus_tag="ACHKTW_003537"
     CDS             complement(771..1721)
                     /gene="corA"
                     /locus_tag="ACHKTW_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HFO6464488.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            complement(1873..1929)
                     /gene="ysgD"
                     /locus_tag="ACHKTW_003538"
     CDS             complement(1873..1929)
                     /gene="ysgD"
                     /locus_tag="ACHKTW_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsgD"
                     /protein_id="HFO6464489.1"
                     /translation="MDTPSRYWLTILSSRINS"
     gene            2091..2855
                     /gene="yigE"
                     /locus_tag="ACHKTW_003539"
     CDS             2091..2855
                     /gene="yigE"
                     /locus_tag="ACHKTW_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HFO6464490.1"
                     /translation="MAHRLLIGKGMITLNLKRIFLALTLLPLFAVAADDCALSDPTLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLADMNSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQKVALNLASGEGNFFIRPSGVFYVAGDKVGIVRLDAFKTSKEIQFAVQSGP
                     MLMENSVINPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYAKAKLNVE
                     QLLYLDGTISHMYMKGGAIPWQRYPFVTMISVERKG"
     gene            complement(3002..5164)
                     /gene="uvrD"
                     /locus_tag="ACHKTW_003540"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             complement(3002..5164)
                     /gene="uvrD"
                     /locus_tag="ACHKTW_003540"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HFO6464491.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPAEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAET
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTLHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLESV"
     gene            complement(5248..5964)
                     /gene="yigB"
                     /locus_tag="ACHKTW_003541"
     CDS             complement(5248..5964)
                     /gene="yigB"
                     /locus_tag="ACHKTW_003541"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HFO6464492.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            complement(5964..6860)
                     /gene="xerC"
                     /locus_tag="ACHKTW_003542"
     CDS             complement(5964..6860)
                     /gene="xerC"
                     /locus_tag="ACHKTW_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFO6464493.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDVTVVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            complement(6857..7564)
                     /gene="yigA"
                     /locus_tag="ACHKTW_003543"
     CDS             complement(6857..7564)
                     /gene="yigA"
                     /locus_tag="ACHKTW_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 family protein"
                     /protein_id="HFO6464494.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            complement(7561..8385)
                     /gene="dapF"
                     /locus_tag="ACHKTW_003544"
     CDS             complement(7561..8385)
                     /gene="dapF"
                     /locus_tag="ACHKTW_003544"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFO6464495.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLAEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            complement(8422..8625)
                     /gene="yifL"
                     /locus_tag="ACHKTW_003545"
     CDS             complement(8422..8625)
                     /gene="yifL"
                     /locus_tag="ACHKTW_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFO6464496.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
     gene            8784..8926
                     /locus_tag="ACHKTW_003546"
                     /pseudo
     CDS             8784..8926
                     /locus_tag="ACHKTW_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580503.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
     gene            9088..9408
                     /gene="cyaY"
                     /locus_tag="ACHKTW_003547"
     CDS             9088..9408
                     /gene="cyaY"
                     /locus_tag="ACHKTW_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HFO6464497.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDWDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(9448..11994)
                     /gene="cyaA"
                     /locus_tag="ACHKTW_003548"
     CDS             complement(9448..11994)
                     /gene="cyaA"
                     /locus_tag="ACHKTW_003548"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HFO6464498.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNMPPANQDHDTPLLQQYFS"
     gene            12381..13322
                     /gene="hemC"
                     /locus_tag="ACHKTW_003549"
     CDS             12381..13322
                     /gene="hemC"
                     /locus_tag="ACHKTW_003549"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HFO6464499.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLRALVGAPDGSQIIRGER
                     RGAPQNAEQMGISLAEELLNNGAREILAEVYNGDAPA"
     gene            13319..14059
                     /gene="hemD"
                     /locus_tag="ACHKTW_003550"
     CDS             13319..14059
                     /gene="hemD"
                     /locus_tag="ACHKTW_003550"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HFO6464500.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPRLPDYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTARGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTMVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            14081..15310
                     /gene="hemX"
                     /locus_tag="ACHKTW_003551"
     CDS             14081..15310
                     /gene="hemX"
                     /locus_tag="ACHKTW_003551"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HFO6464501.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQAAQLK
                     QANRQQETLAKQLDEVQQKVATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTAAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPAPAPA
                     PAPAPQGDTPAAAPQGE"
     gene            15313..16509
                     /gene="hemY"
                     /locus_tag="ACHKTW_003552"
     CDS             15313..16509
                     /gene="hemY"
                     /locus_tag="ACHKTW_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0042168 - heme metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HFO6464502.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            17189..17326
                     /locus_tag="ACHKTW_003553"
                     /pseudo
     CDS             17189..17326
                     /locus_tag="ACHKTW_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312758.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
     gene            complement(17434..18669)
                     /gene="aslB"
                     /locus_tag="ACHKTW_003554"
     CDS             complement(17434..18669)
                     /gene="aslB"
                     /locus_tag="ACHKTW_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HFO6464503.1"
                     /translation="MLQQVPTRAFHVMAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYVRHYIAASEPQNEVAFTWQGGEPTLLGLDFYRRAVKLQAKYGAGRKISN
                     SFQTNGVLLDDKWCAFLAENHFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETAASDGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMIDSPQQQVFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPLYLKAMADLL
                     AHGRPASDIMQAHLLVVNK"
     gene            complement(18816..18892)
                     /locus_tag="ACHKTW_003555"
     tRNA            complement(18816..18892)
                     /locus_tag="ACHKTW_003555"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18856..18858),aa:Pro,seq:tgg)
BASE COUNT         4697 a         5027 c         4792 g         4417 t
ORIGIN      
        1 tatttcagct caaaaaatat ctcttttaag ttttcaaaaa tacgctatag gatggagtgc
       61 aacaattcat tttgtagcac aggaccactt cggtcttgat gtaactgata tcaaaaacaa
      121 gacttacagc aaatatagat tttttcgcat atggttcttt ttacaacggc ataaagactt
      181 tgctttcaaa ccttttttca ccaattttaa cactatcgaa agaatcgaaa attatctatg
      241 aaaagcaaac tccagaaaat tatattatgt ttatttcttc tatgctgcat ctataattta
      301 tggacattgc gtcctgtaca gattttgtat acatactctg atgctggcaa ttcagtcttt
      361 ctggtcgttg atcatctgcc atggactgac agcgataaaa taaattggta tctgaagcat
      421 cagaatgaaa ttaaaaatca acatccccta ccagaaggta gttggcatac ctggtatgtc
      481 attgatatcg gtaatggctt cactgactat aaaaaatata tcgaagggcc gtatgaagat
      541 ctctactgtt tcccgacaat taagagcaat gataattgca tcgtgaaaaa ttacttaatg
      601 gttattaatg aataccctta tcgtaacact catattggta ttaacgattt tactgagtat
      661 caattaacac aagaaaataa aatagagcga gttttcaatc cgcatgactt taaataagac
      721 aatttttaga aataagccgg ataaaaaaat agctttatcc ggcactcata ttacagccag
      781 ttcttgcgct taaagtacag atacggtgcc aggcccgcga ggatcataaa gataatcgcg
      841 cctgggtagc cgaaactcca cttcagttct ggcataaact caaagttcat cccatagctg
      901 gaagcaacga gcgtcggcgg caggaatacc acggatacca ccgagaaaat tttgatgatg
      961 cggttctgct cgatgttgat aaaacccatt gccgcctgca tcaggaagtt caccttctgg
     1021 aacagggatt cgttatgcgg cagcagggat tcgatatctc gcaggatttc acgcgcctgc
     1081 tccagttgcc cacccggtaa acgcgccttg cgtaccagga agttgagcgc acgctgggta
     1141 tccatcagac acaggcgaac tttccagccg atatcttcca gttccgccag cgtggagagc
     1201 gcctcatcgt actcatcgcc ctgatgccct tccataatta cccggctcaa ctgctccaga
     1261 tcgctgtaga tattttcaat ttcatctgcc agctgttcga ttttggtttc gaacagatcc
     1321 aacagcagct cgtaggcgtt accgtccacc attgactggc tacgggcgcg catacgatac
     1381 agacggaatg caggcagttc acgctcacgc agagtaaaca gacgaccatc acggatggta
     1441 aatgccacag tggagttacc ggcgtgatct tccgcatctt caaagaagaa gaaggagtga
     1501 atatgcaggc cgtcatcgtc ttcaaagaaa cgtgccgatg cttcgatgtc ttccagttcc
     1561 gggcgggttg ccaggctctg gccaagttca gattgtacgc gcagtcgctc gtcgtcgtcc
     1621 ggttcgacaa gatcaatcca tactgcattt acaaggggtt gtgactcttc gacttccagc
     1681 cgggtcagtc ggttattttc cagttgaaat gcgctcagca tgaccgggac tcccaatgct
     1741 taaaatatcg gacagttcgg tgggcacaca gaaacaaatt gggtttcaga ccattaaaca
     1801 gcctgactca gcgcgacggg aaaaattgag gtcgctgaca accgctaagg ctatcagcaa
     1861 aaagggatag ccttaggagt tgatcctgga tgacaggata gtgagccagt atctactggg
     1921 tgtgtccaag gcgaatgtcc tcttagagtg atcgtgcgcg catgttacgc cagcaaaatt
     1981 ttgccgtcaa cacgcaacag aacaccacag aacaatagtt gaacttcgcg ctttttggta
     2041 ctcgtcaagg tcgctaacag gataaaaaaa tccaacatcc aggacctata atggcgcatc
     2101 ggctactcat tggtaaagga atgatcacct tgaatctcaa acggattttt ctcgccctca
     2161 ccttacttcc cttgtttgct gttgccgctg atgattgcgc actctcagat ccgacgctga
     2221 ccgtacaggc gtataccgtt aatcctcaaa cagagcgggt gaaaatgtac tggcaaaaag
     2281 ccaatggcga agcgtgggga actttacatg ctctgctggc ggatatgaat agtcagggtc
     2341 aggtgcagat ggcgatgaac ggcggcatct atgatgaaag ctatgcgccg ctcggtttgt
     2401 acatcgaaaa cggtcagcag aaggtggcgt taaatctcgc ttcaggtgaa gggaatttct
     2461 ttatccgtcc tagcggcgtg ttttatgtcg cgggagataa agtcggcatc gttcgtctgg
     2521 atgccttcaa aaccagtaaa gagattcagt ttgcggtgca gtcagggcca atgttgatgg
     2581 aaaacagtgt aattaatccg cgtattcatc ccaacgtcgc ctcacgtaaa attcgtaacg
     2641 gtgttgggat taataaacat gggaatgccg tgtttttgtt gagccagcag gcgacaaatt
     2701 tttatgattt tgcctgttat gccaaagcga aactgaacgt tgagcagctg ctttatcttg
     2761 atggcacgat ttcacatatg tatatgaaag gcggggcaat cccgtggcaa cgctatcctt
     2821 ttgtcaccat gatttccgta gagcggaaag gttaaaacga tagttttgtg tcagatactg
     2881 aagatggcgc agatgccgga tgcggcgtaa acgccctatc cggcctacat gacgttgcaa
     2941 tttattgaat atgaatgatt ttttaggcca aataaggtgc gcagcaccgc atccggcaac
     3001 gttacaccga ctccagccgg gcgtatgccg ccaccagcca tttaataccc tggccctgga
     3061 atgccacctg caaacggcta tgctcgccgc tgccttccat attgacgatg gtgccttcac
     3121 caaacttagc gtggcgtaca cgctgaccga gcttgtagcc gctgtcgttc tcgaccatcg
     3181 gcgtccccat ccgttgatgg ctgaccggac gacttaccgt agcgcgcagg cgcacctctt
     3241 ccacacactc ttccggcagc tcgccgataa agcgcgacgg gcgatggtaa acctctttac
     3301 catacagacg gcgggtttcc gcgtaggtca gcgtcagttt ctgcatcgcg cgggttacac
     3361 caacgtaggc cagacggcgt tcttcttcca gacgcccgcc ttcatccagc gacatctggc
     3421 ttgggaacat accctcttcc ataccgacaa taaacacctg cgggaactcc aggcctttcg
     3481 ccgagtgcag cgtcatcaac tgcactgcat cctgccaggt atccgcctgc ccctcgcccg
     3541 cttccagcgc cgcatgggag aggaatgcct gcagcggcat taaatcttca tcttcttcgt
     3601 tgtagctgaa ctggcgcgtt gccgtcacca gttcctctaa gttttcgata cgcgtctggc
     3661 ctttttcgcc cttctcctgc tcgtacatgg tgcgcaggcc ggagtcttta attacccggt
     3721 cagtctgtac atgcagcggc atatcggcag tttcttgcgc taaggcatcg attaattcca
     3781 taaatcgctg taaggcgctg gcagcacgtc cggcgagggc tttttcctgc aacagttcac
     3841 gacatgcctg ccagagtgtt aactggcgat cgcgcgatgt ctgacgtacc acgtccagcg
     3901 tccggtcacc aataccccgc gttggcgtat tcaccacacg ctcaaaggcc gcgtcgtcgt
     3961 tacggttggc aatcaggcgc agatacgaga gcgcgtcttt gatttcctgg cgttcgaaga
     4021 agcgcatccc gccgtaaata cggtacggca tactggcctg caacagtgct tcttccagta
     4081 cacgcgactg ggcgttactg cggtagagaa tggcgcactc ggcaagcgcc ccgccgttgt
     4141 cctgccaggt tttgatgcgg ttaaccacaa aacgcgcttc atcgagttcg ttaaaagcgc
     4201 aatagaggga aataggctca ccgtccgcgc catcggtcca cagtttttta cccagacgcc
     4261 cgttattgtt ttcaatcagg gcgttagcgg cgctcagaat attgctggta gagcggtagt
     4321 tttgctccag acgaatagtt tcggcaccgg ggaaatcatt aaggaaacgc tgaatattct
     4381 ccacctgcgc cccgcgccag ccgtagattg actggtcgtc atcaccgacg atcatcactt
     4441 tgccggtgtc gcccgccagc aggcggatcc acgcgtactg aatgttgttg gtatcctgga
     4501 attcgtccac caggatattg gtaaaacgtt cgcggtagtg ttgtaggata tgcggcttgt
     4561 taagccacag ttcgtgagcg cgcagcagca gctcggcgaa gtccaccagg cccgcgcggt
     4621 cacacgcttc ctgatacgcc tgatacacct tctgccaggt ctgctccgcc ggattaccgt
     4681 agctttgaat atgatgcgga cgcaggcctt catctttctg gctgttgatg taccacattg
     4741 cctggcgcgg cggccactgc ttctcgtcga ggttcatggc tttgatcaga cgcttaagca
     4801 ggcgcagctg gtcctcgcta tcgaggatct ggaaatcctg cggcagattg gcgtccatat
     4861 ggtgcgcacg cagcaggcgg tgcgccagcc cgtggaaggt gccgacccac atgccgccct
     4921 ggctggtgcc catcagttgc ccgatacgat gacgcatctc cgccgccgct ttgttggtaa
     4981 acgtcaccgc cataatcgag tatggcgagc agttttccac gctcatcagc caggcgatac
     5041 gatgcaccag tacgcgcgtc ttaccactgc ccgcgcccgc cagcaccaga aggttgctgc
     5101 gtggcgcggc caccgcttcg cgctgtttgt cattaaggct gtcgagcagg taagaaacgt
     5161 ccattggcac cgccgcgtaa aaataggttg ggcggagaag cgcaacgccc tccgccaaaa
     5221 agctgggtat atatacagat ttgctgatta tatcagcgag gtcagagatg ccaaccggga
     5281 aatttccaga tgcggcagta aacggctgtc ccaggtttgc atcagatcgc cattttccgg
     5341 tctgatccaa caggcctgca ttccgctgcg aattgcccca cccacgtcag tggtgagatc
     5401 gtccccaaca tgtaagatct cgccgatcgg cacgttgagt ttttccgcag ccagaaagta
     5461 catatcgctg aacggttttg agcgcccgtg cgggccagcg cgcagcacaa actcaaaata
     5521 atcccccaaa ccaaacagtt ccggctgggc gttaccgttg gtgatcgcca ccagcggcca
     5581 tttcttcgcc agctgtttta aggtgtcgtg agtttgctgc gggacgtcga ttcggctgcg
     5641 ccatttggca aagttgatca ttgctgcgtg tgcgcctgca ctggcttctt cggcactcag
     5701 cccggcgtcg agcatcgctt gttcaatcga acgaaaacgc caacgcgtca cgtcgtgata
     5761 aatctcgggt tccgcttccc gtaccgcctg gcgcaggcgt tgcagatctt cattctggaa
     5821 gctgcgcagc gccggatgat aattttgcac aaaggtaagc gcctctcgct cggtgcgcaa
     5881 aatcaccgga cggttatcgt aaagggtatc atccaggtca aaggtgagcg ccgagatgcg
     5941 ccccaaaggc cggtaaaaac gcattatttc ccccgtttgg cgcgtggatg cgccgcatcg
     6001 tacaccgagg caaggtgttg aaaatcaaga tgagtataga tttgcgtggt ggagaggttg
     6061 gcatgaccca gtagctcctg cacaccacga agatcgccgc tcgactccag catatgcgta
     6121 gcgaacgagt gacgtaattt atgtgggtga acgtgattat tcagcccttg ttttatgccc
     6181 cattcggcaa agcgtttctg cacattacgc gcggagatac gcttgcccag tttcgacaga
     6241 aaaagcgcgt cgtcttcgct accaaacagg tcgcgcaaat caagccagtg ctcaatccac
     6301 gccacagcgt tgcgaccaat cggcaggcgg cgctctttgc tgcctttccc cataacccac
     6361 acttcaccag actccaggtc gaggtgttta atatccagcc ccaccagctc agaaagacgc
     6421 agacccgcgc cgtacatcac ttccagcatt gcacggtcgc gtacagcgag gggatcattg
     6481 atatcaatat ccagcagccg attcatatcg tcgacgtcga tgtttttcgg cagatgacgc
     6541 ggcgctttcg gtgccgaaac acctttcgcc gggttagctt tgagttcgtt ctggctgacc
     6601 agccagtcaa aaaagctacg tagcgcagaa agccgtaacg ccagacttgc tgctcccagc
     6661 cctttacggc gactgcgtac agcaaaattg cgcaccaccg tcacatcaca ttgctgccag
     6721 ctttgcaggc cgttttcgct ggcaaaattg atgatcgcct caagctgacg ctggtagtta
     6781 agcagggtta tcgggctaag ctggcgctcc acgctcagat aacgtaggta gcgttctaca
     6841 tcggtgtgta aatcggtcat acgcgttcaa tccaacgctc cagaagctcc ggcaacatca
     6901 gcgcaatttc atgaagtaac tgcgttcctt gcccttgttg atagtgactg gcatcgcgac
     6961 tggtaaacag cacaacaccc aaatcagcat cgcttcccag catcgacatc gccaccgatc
     7021 ccactgcttt cgcttcaggt agcaccacca gcagctcagg tccgttaagc ggcccaagat
     7081 agtgctgttc ctgccccaaa cgctgaatac gcagcggttc gaaagactga cggcttaatg
     7141 ccagatgagt gtggttcgac ggcgcaccta agcgccagcg atccggaaac aggcgcagac
     7201 tcgcacctgc caggccgaga tcgcgcgccc agcggtgaaa gcgcatcagc atatcgtcga
     7261 gactgctggc ggcggtgaga ctgcgctgca ggtagagtag gcgataaaac aggccttcgt
     7321 tggcgatagc ctgttccatc aacagcgcca tgttctcttc cagaacatga atatgattac
     7381 gtgcgcgggc catgtgccac tcgaccaacg aaacggtgcc gcgtaccgga tgcggcacac
     7441 gtatcgcttc tactgcgcgc gcattacgga taaaaaactc aggattttta atcagataat
     7501 cgacaaccgc ccggtcatca agctccgtga gtgtttcctg cagttcttcc cctggttgct
     7561 tcatagatga ataaatccgt cgtagacatg taccgccggg ccagtcatat ataacgggtg
     7621 acccggacct ttccaggcga tatcaagacg accgccgggg agttccacgc gtacttcttc
     7681 ggccagcaaa ccttgctgaa tccctactgc aaccgccgca cacgcgccgc tgccgcaggc
     7741 ctgggtttct cctgccccac gctcataaac gcgtaaacga atatgctcgc gcttaaccac
     7801 ttgcataaaa ccgatattgg cgcgctccgg aaaacgctcg tggctttcca gaacaggacc
     7861 aagcgtttct accgccgcgg tatcgacatc atcgacctga atcacgcaat gcggatttcc
     7921 catcgacacc acgccgcata agattgtctg ctcggcggcg cgcataatat aggtcttttc
     7981 cgctttgtta gcgcgaaacg gcacggcgga aggttcgaag ttgggttcgc ccatatttac
     8041 gcggaccaga tcatcatcgg tgacggtcag aaccatccgc ccgttggcgg tgctgacgcg
     8101 gatatcacgc ttattggtca gtcctttcag acgcacaaaa cgggcaaagc agcgcgcacc
     8161 gttgccgcac tgcgccactt cactgccatc agcattgaaa atgcgatagt gaaaatccag
     8221 ttcaggatca tacggcggct caaccaccag cagttggtca aaccctaccc ccaggtgccg
     8281 atcagccagg cgacgaatca gctccggtga aaaaaagaca ttctgcgtta ccgcgtcgac
     8341 gaccataaaa tcgttgccaa ggccatgcat tttcgagaac tgcatcattt actccaatca
     8401 cgcgggtaca gaaactgact tttagtaatt cacctgggat ggaccatcgc cagtggcgcg
     8461 atcgttttta tccggcaccg tggattgcgt ttgcgtctct accggtttgg tcggcggcgg
     8521 tgcgttttta tctgcaggcg ggaaatagag cggacctttc agaccgcagc ccgtcaggct
     8581 gaagagagta agtaatacag tgagtgcctt aaacacgttt ttcattatgg attgcctgta
     8641 aagttcatcg ctttctgctt tctatcatcg caggagaaga cgtaaaagca agcgtttagc
     8701 gactctttat gaagtctgaa aagtgaggaa atgctgcgag tataacgctt actcgtgggg
     8761 atttatgttt tgctggtagt ttcgtgatcg gcacctcgcg ccaggatggc tttatgcact
     8821 atcgcaatgt agtttgtttg ttggcttttt tagttccgtc agcgtgagga tgctcgctcg
     8881 gtaaacctga tcataatgtt taccagaaac agggaaaggc gtggtgtaac tgcgccctaa
     8941 tgaagagaca aatctttcgc tacactgaga catcattatt ccggtgacgg atgaatcgtg
     9001 ccggaacgca gccgcagagg tgcaaacttt gaagccctga ccccatgccg ctatactgcc
     9061 gccatcactt aaaaacagga tacaacaatg aacgacagtg aatttcatcg cctggctgat
     9121 caactctggc tgaccattga agaacgcctg gacgactggg atggcgacag cgatatcgac
     9181 tgcgaaatca acggcggcgt actgaccatt acctttgaaa acggcagcaa aatcattatt
     9241 aaccgccagg agccgctgca ccaggtgtgg ctggcaacca aacagggcgg ctaccatttt
     9301 gacctaaaag gcgatgaatg gatttgcgat cgcagcggcg aaaccttctg ggatttgctg
     9361 gaacaggcgg cgacacagca agcgggtgaa acagtcagtt tccgctaaga ttgcatgccg
     9421 gataagcctc gctttccggc acgttcatca cgaaaaatat tgctgtaata gcggcgtatc
     9481 gtgatcctga ttggcaggcg gcatgttacc gatagatttt gtgcggaacg gaatcacctg
     9541 ttcacgacca tcaaccttca caatctgata gaactgcggc aggttgaagt tgatgaagct
     9601 tgagccgtag gtgaagcggt cgtgcgatga cgagtagaag cgactgacgt cacgcaccag
     9661 ttcctctttg ctgccttcgc agtggtgata cacctcaacc cggttgcttt cgtcgagaat
     9721 gtagatatta aagccattct cgtcttgcgt ttcttcgaag aagaactgga tgatcccttc
     9781 actagcaaag ccgtccacca ccgccggtaa tttgacgtga ttggtttcaa cctgcactga
     9841 caagccgtgc agtttgttat gcgaaatagc gccataaaac tcgatggcgt tttccagttt
     9901 ttgtaccgat acattcaggc gttcgaagaa caacccccag gtttgaccag aaacgcgcag
     9961 cgccttgaaa cgcccggttt cctggcgggt gctggaaagg cgcagttcaa tacactcaga
    10021 aaccagttgc tgcacgcgag tacggattaa cccgcgcaga tgctggctat aacagaagac
    10081 ttccacgcta tctggcggtg cggcgtcctg atgcattttg ccgagaatag ttttcagggc
    10141 ttcgatcatc gattgctcgc cgttgaagtg cagcgtacgc acttcgttcc acgagttgcg
    10201 gtacagcagg tcaacgctac ctaccaggca attttgattc tcgccaaagc tgaagacatc
    10261 cagcttacgg aaatcaaaat gcaccacctg attgcggaac gccgctgtcg ggtcatattc
    10321 caggttaacg ataatcgcca gatggcggat ctcacacggg ctgtagagcg ccttcggtgt
    10381 cggtgcaggt aagcgcagcg ggaaatggtg cgacacgtcg gcgaccatct cctgcaactt
    10441 aggcaaatcg acaatgccgt tacctttaat atacaaacgg gtgcgcgagg tcagcaggcc
    10501 gttaaaccat gcccacgcca ccagtttgtt caggtaacgg ttatattcca gcggctgatg
    10561 gctgatgatc gactcaatat ttggcgcgcg gttatacaga taccaacctg aacggttagc
    10621 acggcccggc ggcacataaa taaaggtcag attcggttcc gagagatcgg gtgaaatctg
    10681 cgggtttacc agcgtcactt tacctggtaa tgcttcaaac gcggcataca gcttacgcgt
    10741 cagcacgccg atatcctgcg gactggcgga gacgctaagg ttattgcgac gcgcaaagcg
    10801 gatcagatta cggtagctct gcatcatcgc gtcgagcaac tcgttgtgcg cctcacgcac
    10861 ctgatcaatc ttccagttag cgcggttatc gagcattgcc agacgagctt cgtcccaacc
    10921 ccactcgctc actaactggc tcaacactgc gcgacgccag cctacgcagg cgcgttcacg
    10981 gctgagcttt tcgcacactt ttaaatagaa gcagcgacgt actaaatcca gacgggtaaa
    11041 atcttcaatc gccgtcaggt attcagtaac acgctccagc atcatgcagt atggatcgag
    11101 accaaacgat acaatctcgc cgtcgtgcaa acgctgtttg atatctttcg ccagcagacg
    11161 tgggttcggg tattcccagg aataggcttc cagcagcagt gttttcagta ccgctttgta
    11221 tggggaatcg atactcttgt agagctgcca aaggctggca ccaaagtact cttcagcaga
    11281 aagcgagctt aagccaccga gatccagcca ttcatttggc gtcagcacgc cctgcgcgta
    11341 aagcgtcatc acatagtcgt cgtaatgctc ttcttcgtcg cacggcacca tattccacag
    11401 aatacgctta ccggcgagac gcacggcggt acgataaaat tcgtcaagca gcagtatatg
    11461 ctgggtggag ccacaatctt cgccccccag gctgccgctt tcattatgac ggaagcggtt
    11521 ttcatcaatc aggaagaagc tgacttccac acccagcgag gcggcccagc tttccagcag
    11581 actacattta cgttgtagta actggcgctc ttcgctatcg agccaggatt gatgacagac
    11641 ccagatatcc aggtcagagg aacaactttg ccctacggac gaggtgctgc ccatggtgta
    11701 tacaccagta attggaagct cacctttcgg tgggtcctgt actgacattc cacgatacag
    11761 ttcaagctcg ttcaggtagt ggcgttgagt ttcatcaggc gtgtaaaggc aaatgccttt
    11821 gggaacgtta ccatcaaggt aacccggcat tagcggatgg tgatagtgca acaatgtcgg
    11881 cagtagactg tagacctgtt ggaatgcagg ccccatagca gcaagcgcgc gatccacacg
    11941 caattgattt atggcatcca gtctctgttt cagagtctca atatagaggt acaagacgta
    12001 tcgcctgatt tgctacccgt catgactgtg attccgccaa catcaacggt aacacgcggc
    12061 attcgggata tttcgtatgt caaaggtaac cgttaccact tttcgcgcct ggttttttta
    12121 gtttcacgac gaaaaaatgg tctaaaacgt gatcaattta acaccttgct gattgaccgt
    12181 aaagaaagat gcgctacata caagtgtagc accgtttatt ctctgtaaat tccttattac
    12241 gacggcgtga aacgcctgtc aggatccact gccagacctc attttacggt ttgcgcaggc
    12301 gtctacgttt caccacaaca ctgacatcac tctggcaagg atgttaggat ggaccacgga
    12361 tgataatgac ggtaacaagc atgttagaca atgttttaag aattgccaca cgccaaagcc
    12421 cacttgcact ctggcaggca cactatgtca aagacaagtt gatggcgagc catccgggcc
    12481 tggtggttga actggtaccg atggtgacgc gcggcgatgt gattcttgat acgccgctgg
    12541 cgaaagtagg cggaaaaggc ttatttgtaa aagagctgga agtcgcgctc ctggagaatc
    12601 gcgccgatat cgccgtacat tcaatgaaag atgtgccggt tgaattcccg caaggtctgg
    12661 ggttggtcac tatttgtgag cgtgaagatc ctcgcgatgc ctttgtgtcc aataattatg
    12721 acagtctgga tgcgttaccg gcaggcagta tcgtcgggac gtccagttta cgtcgccagt
    12781 gccaactggc tgaacgccgc ccggatctga ttatccgctc cctgcgcggc aacgtcggca
    12841 ctcgcctgag caaactagat aacggcgaat acgatgccat cattcttgcc gtagccggac
    12901 taaaacgttt aggtctggag tcccgcattc gcgccgcgtt accacccgag atttctcttc
    12961 cggcggtagg acaaggtgca gtcggtattg aatgccgcct tgatgatacg cgcactcgcg
    13021 agctgcttgc cgcgctgaat caccacgaaa ctgcactgcg cgttaccgca gaacgcgcca
    13081 tgaatacccg tctcgaaggt ggatgtcagg tgccaattgg tagctacgcc gagcttattg
    13141 atggtgaaat ctggctgcgt gcgctggtcg gcgcgccgga cggttcgcag attattcgcg
    13201 gtgaacgccg cggtgcgccg caaaatgccg aacaaatggg gatttcgctg gcagaagagc
    13261 tactgaataa cggcgcgcgc gagatcctcg ctgaagtcta taacggagac gccccggcat
    13321 gagtatcctg gtcacccgcc cgtctcccgc tggagaagag ttagtgagcc gtctgcgcac
    13381 actggggcag gtggcctggc attttccgct gattgagttt tctccgggtc gacaattacc
    13441 gcaacttgct gatcaactgg cggcgctggg ggagagcgat ctgttgtttg ccctctcgca
    13501 acacgcggtt gcttttgccc aatcacagct gcatcagcaa gatcgtaaat ggccccgact
    13561 acctgattat ttcgccattg gacgcaccac cgcactggcg ctacataccg taagcggaca
    13621 gaagattctc tacccgcagg atcgggaaat cagcgaagtc ttgctacaat tacctgaatt
    13681 acaaaatatt gcgggcaaac gtgcgctgat attacgtggc aatggcggtc gtgagctaat
    13741 tggggatacc ctgacagcgc gcggtgctga ggtcactttt tgtgaatgtt atcaacgatg
    13801 cgcaatccat tacgatggtg cagaagaagc gatgcgctgg caatcccgcg aggtgacgat
    13861 ggtcgttgtt accagcggtg aaatgttgca gcaactctgg tcgctgatcc cacaatggta
    13921 tcgtgagcac tggttactac actgtcgact attggtcgtc agtgagcgtt tggcgaaact
    13981 cgcccgggaa ctgggctggc aagacattaa ggtcgccgat aacgctgaca acgatgcgct
    14041 tttacgggca ttacaataac tctcataaca ggaagccata atgacggaac aagaaaaaac
    14101 ctccgccgtg gttgaagaga ccagggaggc cgtggacacc acgtcacaac ctgtcgcaac
    14161 agaaaaaaag agtaagaaca ataccgcatt gattctcagc gcggtggcta tcgctattgc
    14221 tctggcggcg ggcgtcggtt tgtatggctg gggtaaacaa caggccgtca atcagaccgc
    14281 caccagcgat gccctggcta accaactgac ggcattgcaa aaagcccagg agagccaaaa
    14341 agccgagctg gaaggcatta ttaagcaaca agctgcacag cttaagcagg cgaatcgtca
    14401 gcaagaaacg ctggcaaaac agttggatga agtccaacaa aaggtcgcca ccatttccgg
    14461 cagcgatgct aaaacctggc tgctggctca ggccgatttt ctggtgaaac tcgccggacg
    14521 gaagctgtgg agcgatcagg acgtcacgac cgctgcagcg ttgctgaaaa gtgcagatgc
    14581 cagcctggcg gatatgaatg acccgagtct gattaccgtt cgtcgggcaa ttaccgatga
    14641 tatcgccagc ctttctgcag tttcgcaggt ggattatgac ggcatcatcc ttaagcttaa
    14701 tcagctttca aatcaggtag ataacctgcg tctggccgat aatgacagcg atggttcgcc
    14761 gatggattcc gacggtgaag agctttccag ttccatcagc gaatggcgta tcaatctgca
    14821 aaaaagctgg cagaacttta tggacaactt cattacgatt cgccgtcgtg atgacaccgc
    14881 cgtaccgctg ttagcgccaa atcaggatat ctatctgcgc gaaaatattc gctctcgcct
    14941 gctggtcgca gcacaagctg taccgcgtca ccaggaagag acttatcgcc aggcgctgga
    15001 gaacgtctcc acctgggtac gagcttacta cgatactgat gatgccacca ccaaagcgtt
    15061 cctcgacgag gtggaccagt taagccagca aaatatctcg atggatcttc cggaaaccct
    15121 gcaaagccaa gcgatgctgg aaaaactgat gcagacccgc gtgcgtaacc tgctggcaca
    15181 accggcagcg gggacaacgg aagctaaacc tgcacctgca cctgcacctg cacctgcacc
    15241 tgcacctgca cctgcacctg cacctgcacc gcaaggtgat actccggcag ccgcgccgca
    15301 aggagaataa ccatgctaaa agtgttatta ctctttgttt tgctgattgc ggggatagtg
    15361 gttggcccga tgattgccgg tcatcagggt tacgtgctga tccagactga caactacaat
    15421 atcgaaacca gcgtcacggg cctggcgatt attttgattc tggcgatggt agtgctgttt
    15481 gccattgagt ggctactgcg gcggatcttc cgcacaggcg cacacacccg tgggtggttt
    15541 gtcggacgta agcgtcgccg tgcccgtaag cagaccgaac aggcgctgct gaaactggcg
    15601 gaaggcgatt atcagcaagt tgaaaagctg atggcgaaaa atgccgatca cgcggaacaa
    15661 ccggtggtga actatctact ggctgccgaa gcagcgcaac aacgtggtga tgaagcacgc
    15721 gccaaccaac atctggaacg cgcagcagag ctggccggca atgacaccat tccggtagaa
    15781 atcacccgtg tacgtctgca actggcccgt aatgaaaacc atgctgcacg ccacggcgtg
    15841 gataagctgc tggaagttac gccacgccat ccggaagtat tacgtctggc ggaacaggcg
    15901 tatatccgca caggtgcatg gagttctctg ctggatatca tcccatcaat ggcgaaagcc
    15961 catgttggtg atgaagaaca tcgtgcaatg ctggaacaac aggcatggat tggcctgatg
    16021 gatcaggcgc gtgccgataa cggtagtgaa ggtttgcgta actggtggaa aaaccaaagc
    16081 cggaaaacgc gtcatcaggt agcgttgcag gtggcaatgg cggaacatct tattgaatgt
    16141 gacgatcatg atactgccca gcaaattatc atcgatggcc tgaaacgcca gtacgacgat
    16201 cgcctactgc tgccgattcc tcgactgaaa accaacaatc cggaacagct ggaaaaagtg
    16261 ctgcgccagc agatcaaaaa cgtcggcgat cgcccgctgt tgtggagcac actgggccag
    16321 tcactgatga agcacggaga atggcaggaa gcatcgctcg ccttccgcgc ggcgctgaaa
    16381 caacgtccgg acgcctacga ttacgcatgg cttgccgacg cgctggacag actgcacaag
    16441 ccggaagaag ctgcagcgat gcgtcgcgac ggtttgatgt taacgttgca gaacaaccca
    16501 ccacagtagt tccttctcac ccggaggcaa gcacctccgg gacctttctg atgcataaaa
    16561 aaacgcctgc tcttattacg gagcaggcgt taaaacaggt ctgtatgaca acaagtgggt
    16621 gcttcactca acgttgtgtc catggtgtct gatgaggcat aagcgacatc tgtcagtgga
    16681 cgataagcac cgtaaacggc tctgcgtcat tccggagttt atgaggcact aaggcgaaca
    16741 taagagatgg aatgagcatc tacccgttta ttatgccaca gagaatcggg aaataacatc
    16801 ccttaacact tgttatgaga taattctgta atcctctttg cttcctgagt aataacttcc
    16861 tgagtgaata tttaacctga gcttgatcct acacatactt attatgaatg ataaaattca
    16921 ttcaattaat aacacatata ttaattgccg ttaaaactaa aaaaaagcat caataatcaa
    16981 cgcgatataa taaacctgcc ttacatatca actgcgccag aggtaggatt gaaaacgctc
    17041 tcctgatttt ccaattcatt ttctggataa ataaataatt tatttttgtc actattattt
    17101 atgtaatcat cctgtcaggg agagggatct caattatcaa tgcttaatta cgtcatcatt
    17161 ttgatgacat gaaaccggag cattttgcat ggaattttcg ttttcaccca aacttcttgt
    17221 tgttgctgtc gccgccgctc ttcctctcat ggccagcgca gcagataccc cgtcaactgc
    17281 caccgcacgc aaaggctttg ccggatacga tcacccaaac cagtatccac agaacgctgc
    17341 aatttattga atttgcacga tcatgtaggc cggataaggc gtttacgccg catccggcaa
    17401 tcaaccgcag gcggccacaa aatggccgct tttttactta ttcaccacca gcaaatgtgc
    17461 ctgcataatg tcactggccg ggcgaccatg cgccagcaaa tcagccattg ctttaagata
    17521 tagcggtaga tggcggaaat aacgctgata cccggcacac aaataattca gccccggttt
    17581 gccgctggca tcgagcatga agcggtgttt cgggcagcct ccccagcacg cttttaacac
    17641 gttacaactg cgacactgcg ccggtaattg cttaaattta tcttcaccaa acacctgctg
    17701 ttgcggggaa tcgatcattt ctgcaattgt ttgctgatgc atattcccca gccgatattg
    17761 cggataaaca tagtgatcgc aggcgtaaac gtcgccgttg tgctcaacaa tcaccgagcg
    17821 cccacaggtt ggctgatgat ggcaaaccgc acccggcgca ccgacaaaat tggcaaacgc
    17881 ccattcgata ttcatcacga aaatcttgcc gacgtcgcgt ttgatccagt ggtcgaatat
    17941 cgccaccaga aattcaccaa attcatcggg gcgcaccgac cattccgtca gttccccctg
    18001 aatatcacca ggcgcatgta gtttcagtcc atcgctggca gctgtttcat cagccaggcg
    18061 ctcgaccacc ggaataaact ggatgaattc gactcccgca tcgcacaaaa aatcataaac
    18121 ctgcaacggt tgctgcgcgc tggtgcggtt gacgcagacc agcacgttat agtcgacatg
    18181 atgtttttgc agcagcgtca gggcacgcat caccagctta tgcgttggtc tgccaccttt
    18241 ggtcacgcga tattgattgt ggatctcagc cggaccgtcc agcgataacc caacaagaaa
    18301 atgattttct gccagaaatg cacaccattt atcatcgagc agcacgccgt tagtctggaa
    18361 gctgttactt atcttcctgc cagcaccgta tttcgcctgt aactttacgg cacggcggta
    18421 aaaatccagc ccgagtaacg ttggttcgcc gccctgccag gtaaaagcca cttcgttttg
    18481 cggttcgctg gcagcgatat agtgacggac atacgcttcc agcgtgtcat cgtccatatg
    18541 cgtgactggc ttttcgcggt aaagggattg tttttcgaga taaaaacagt agtcacagtt
    18601 cagattacaa tcggaaccac tcggtttcgc catcacatga aaagcacgcg ttggaacctg
    18661 ttgcagcatg gtcactcctt gcctgaagtc atccttcaat acgttaaggg cgaaatcata
    18721 ccgtggggca atgagttatt acgggcgttt attgcgaagg gagggtgacg aaatgcacag
    18781 aaaacaaaaa accccgccga agcggggttc aaaattggtc ggcgagagag gattcgaacc
    18841 tccgacccac tggtcccaaa ccagttgcgc taccaagctg cgctactcgc cgaaatactg
    18901 ctttttgaat ttttagttca attctttaaa gtc
//
LOCUS       DAZFQD010000103        18535 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.103, whole genome shotgun sequence.
ACCESSION   DAZFQD010000103 DAZFQD010000000
VERSION     DAZFQD010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18535
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.103"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(162..710)
                     /gene="pgsA"
                     /locus_tag="ACHKTW_003556"
     CDS             complement(162..710)
                     /gene="pgsA"
                     /locus_tag="ACHKTW_003556"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFO6464504.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYAGIALFFVA
                     AVLTLWSMLQYLSAARADLLEQ"
     gene            complement(767..2599)
                     /gene="uvrC"
                     /locus_tag="ACHKTW_003557"
     CDS             complement(767..2599)
                     /gene="uvrC"
                     /locus_tag="ACHKTW_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFO6464505.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAARIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(2596..3252)
                     /gene="uvrY"
                     /locus_tag="ACHKTW_003558"
     CDS             complement(2596..3252)
                     /gene="uvrY"
                     /locus_tag="ACHKTW_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HFO6464506.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     TNAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            3394..3465
                     /locus_tag="ACHKTW_003559"
                     /pseudo
     CDS             3394..3465
                     /locus_tag="ACHKTW_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001302039.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            3548..3724
                     /gene="yecU"
                     /locus_tag="ACHKTW_003560"
     CDS             3548..3724
                     /gene="yecU"
                     /locus_tag="ACHKTW_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YecU"
                     /protein_id="HFO6464507.1"
                     /translation="MIKIFIGHYINVFYSTADITLKKQPLLFLAKLMVYSAALTFFTA
                     NFHCNMTRKINEYA"
     gene            3711..3935
                     /gene="yecF"
                     /locus_tag="ACHKTW_003561"
     CDS             3711..3935
                     /gene="yecF"
                     /locus_tag="ACHKTW_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein YecF"
                     /protein_id="HFO6464508.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(4002..4724)
                     /gene="sdiA"
                     /locus_tag="ACHKTW_003562"
     CDS             complement(4002..4724)
                     /gene="sdiA"
                     /locus_tag="ACHKTW_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
                     /protein_id="HFO6464509.1"
                     /translation="MQDTDFFSWRRTMLLRFQRMEAAEEVYHEIELQAQQLEYDYYSL
                     CVRHPVPFTRPKVAFYTNYPEAWVSYYQAKNFLAIDPVLNPENFSQGHLMWNDDLFSE
                     AQPLWEAARAHGLRRGVTQYLMLPNRALGFLSFSRCSTREIPILSDELQLKMQLLVRE
                     SLMALMRLNDEIVMTPEMNFSKREKEILKWTAEGKTSAEIAMILSISENTVNFHQKNM
                     QKKINAPNKTQVACYAAATGLI"
     gene            complement(4954..5706)
                     /gene="tcyN"
                     /locus_tag="ACHKTW_003563"
     CDS             complement(4954..5706)
                     /gene="tcyN"
                     /locus_tag="ACHKTW_003563"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     TcyN"
                     /protein_id="HFO6464510.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPQQPRTRQFLEKFLLQ"
     gene            complement(5703..6371)
                     /gene="tcyL"
                     /locus_tag="ACHKTW_003564"
     CDS             complement(5703..6371)
                     /gene="tcyL"
                     /locus_tag="ACHKTW_003564"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HFO6464511.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSLIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(6386..7372)
                     /gene="dcyD"
                     /locus_tag="ACHKTW_003565"
     CDS             complement(6386..7372)
                     /gene="dcyD"
                     /locus_tag="ACHKTW_003565"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019148 - D-cysteine desulfhydrase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HFO6464512.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDDVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCDALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEILLWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            complement(7477..8277)
                     /gene="tcyJ"
                     /locus_tag="ACHKTW_003566"
     CDS             complement(7477..8277)
                     /gene="tcyJ"
                     /locus_tag="ACHKTW_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015276 - ligand-gated monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO6464513.1"
                     /translation="MKLAHLGRQALMGVMAVALVAGMSVKSFADEGLLNKVKERGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTAADLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            complement(8365..8916)
                     /gene="fliZ"
                     /locus_tag="ACHKTW_003567"
     CDS             complement(8365..8916)
                     /gene="fliZ"
                     /locus_tag="ACHKTW_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HFO6464514.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDMLLDENGWQVEQKSWAALCRFCGDLHCKTQSDFFDIIGFKQFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLDPLQDGFLDEILAPWLPTTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSSSLPASDIY"
     gene            complement(8962..9681)
                     /gene="fliA"
                     /locus_tag="ACHKTW_003568"
     CDS             complement(8962..9681)
                     /gene="fliA"
                     /locus_tag="ACHKTW_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HFO6464515.1"
                     /translation="MNSLYTAEGVMDKHSLWQRYVPLVRHEALRLQVRLPASVELDDL
                     LQAGGIGLLNAVERYDALQGTAFTTYAVQRIRGAMLDELRSRDWVPRSVRRNAREVAQ
                     AIGQLEQELGRNATETEVAERLGIDIADYRQMLLDTNNSQLFSYDEWREEHGDSIELV
                     TDDHQRENPLQQLLDSNLRQRVMEAIETLPEREKLVLTLYYQEELNLKEIGAVLEVGE
                     SRVSQLHSQAIKRLRTKLGKL"
     gene            complement(10002..11654)
                     /gene="fliC"
                     /locus_tag="ACHKTW_003569"
     CDS             complement(10002..11654)
                     /gene="fliC"
                     /locus_tag="ACHKTW_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin FliC"
                     /protein_id="HFO6464516.1"
                     /translation="MAQVINTNSLSLITQNNINKNQSALSSSIERLSSGLRINSAKDD
                     AAGQAIANRFTSNIKGLTQAARNANDGISVAQTTEGALSEINNNLQRIRELTVQASTG
                     TNSDSDLDSIQDEIKSRLDEIDRVSGQTQFNGVNVLAKDGSMKIQVGANDGQTITIDL
                     KKIDSDTLGLSGFNVNGGGAVANTAASKADLVAANATVVGNKYTVSAGYDAAKASDLL
                     AGVSDGDTVQATINNGFGTAASATNYKYDSASKSYSFDTTTASAADVQKYLTPGVGDT
                     AKGTITIDGSAQDVQISSDGKITSSNGDKLYIDTTGRLTKNGFSASLTEASLSTLAAN
                     NTKATTIDIGGTSISFTGNSTTPNTITYSVTGAKVDQAAFDKAVSTSGNDVDFTTAGY
                     SVDGATGAVTKGVAPVYIDNNGALTTSDTVDFYLQDDGSVTNGSGKAVYKDADGKLTT
                     DAETKAATTADPLKALDEAISSIDKFRSSLGAVQNRLDSAVTNLNNTTTNLSEAQSRI
                     QDADYATEVSNMSKAQIIQQAGNSVLAKANQVPQQVLSLLQG"
     gene            11920..13326
                     /gene="fliD"
                     /locus_tag="ACHKTW_003570"
     CDS             11920..13326
                     /gene="fliD"
                     /locus_tag="ACHKTW_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0009421 - bacterial-type flagellum
                     filament cap [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HFO6464517.1"
                     /translation="MASISSLGVGSGLDLSSILDSLTAAQKATLTPISNQQSSFTAKL
                     SAYGTLKSALTTFQTANTALSKADLFSATSTTSSTTAFSATTAGNAIAGKYTISVTHL
                     AQAQTLTTRTTRDDTKTAIATSDSKLTIQQGGDKDPITIDISAANSSLSGIRDAINNA
                     KAGVSASIINVGNGEYRLSVTSNDTGLDNAMTLSVSGDDALQSFMGYDASASSNGMEV
                     SVAAQNAQLTVNNVAIENSSNTISDALENITLNLNDVTTGNQTLTITQDTSKAQTAIK
                     DWVNAYNSLIDTFSSLTKYTAVDAGADSQSSSNGALLGDSTLRTIQTQLKSMLSNTIS
                     SSNYKTLAQIGITTDPSDGKLELDADKLTAALKKDASGVGALIVGDGKKTGITTTIGS
                     NLTSWLSTTGIIKAATDGVSKTLNKLTKDYNAASDRIDAQVARYKEQFTQLDVLMTSL
                     NSTSSYLTQQFENNSNSK"
     gene            13351..13761
                     /gene="fliS"
                     /locus_tag="ACHKTW_003571"
     CDS             13351..13761
                     /gene="fliS"
                     /locus_tag="ACHKTW_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HFO6464518.1"
                     /translation="MYAAKGTQAYAQIGVESAVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSLIQDPV"
     gene            13761..14126
                     /gene="fliT"
                     /locus_tag="ACHKTW_003572"
     CDS             13761..14126
                     /gene="fliT"
                     /locus_tag="ACHKTW_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001015018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:1902209 - negative regulation of
                     bacterial-type flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HFO6464519.1"
                     /translation="MNHAPHLYFAWQQLVEKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEINPSTTMQEQLRPMLRLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNFS"
     gene            14204..15691
                     /gene="amyA"
                     /locus_tag="ACHKTW_003573"
     CDS             14204..15691
                     /gene="amyA"
                     /locus_tag="ACHKTW_003573"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HFO6464520.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSKFIWDFKCF
                     SGIDHIENPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTINLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(15725..16138)
                     /gene="yedD"
                     /locus_tag="ACHKTW_003574"
     CDS             complement(15725..16138)
                     /gene="yedD"
                     /locus_tag="ACHKTW_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310694.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YedD"
                     /protein_id="HFO6464521.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRVIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            16325..17530
                     /gene="yedE"
                     /locus_tag="ACHKTW_003575"
     CDS             16325..17530
                     /gene="yedE"
                     /locus_tag="ACHKTW_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HFO6464522.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFTALMLIIGWEKRFFRRA
                     APQTAKEIA"
     gene            17527..17760
                     /gene="yedF"
                     /locus_tag="ACHKTW_003576"
     CDS             17527..17760
                     /gene="yedF"
                     /locus_tag="ACHKTW_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HFO6464523.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            17869..>18535
                     /gene="yedK"
                     /locus_tag="ACHKTW_003577"
     CDS             17869..>18535
                     /gene="yedK"
                     /locus_tag="ACHKTW_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HFO6464524.1"
                     /translation="MCGRFAQSQTRVDYLALLAEDIERDIPYDPEPIGRYNVAPGTKV
                     LLLSERDEHLHLDPVFWGYAPGWWDKPPLINARVETAATSRMFKPLWQHGRAICFADG
                     WFEWKKEGDKKQPYFIYRADGQPVFMAAIGSTPFERGDEAEGFLIVTAAADQGLVDIH
                     DRRPLVLSPEAAREWMRQEIGGKEASEIATNGCVPANQFTWHPVSRAVGNVKNQGAEL
                     IQPV"
BASE COUNT         4604 a         4784 c         4522 g         4625 t
ORIGIN      
        1 ctattcccgc attcatcaag caatcagtta atcacttgat tttattatcg tctggcaatc
       61 agtgccgccg ttcgatgcgt tgcattctac ttacctggcg cgatgagtca acgatatttt
      121 tcaccacttt tgattgtttg ctgaaaatta cgccgaaacg atcactgttc aagcaaatct
      181 gcacgcgcag cgctcaaata ttgcaacatt gaccacagag tcagtaccgc agccacaaag
      241 aaaagtgcaa taccggcgta ctcaacccaa atgttcggac gccacagcag ccaggccaac
      301 gccaccatct gggcagtggt tttcactttc ccaatccagg agacagccac gctactgcgt
      361 ttacccaact ccgccatcca ttcgcgtagc gcagaaataa taatttcacg ggcgatcatc
      421 gttgccgccg gtaaggtcac ccaccagctg tgataatgct cggttaccag caccatggcg
      481 atagccacga gaactttatc tgcgacaggg tcaaggaaag caccaaaccg ggtactctgg
      541 ttccagcggc gtgccagaaa accatcgaac cagtcagtca ccgccgcgac gcagaaaatg
      601 agcgcggcgg caaacggcga ccaggtgaca ggcagataaa agaccaatac aaagaatggg
      661 ataaggatga cacggaacag tgtaagcaac gtagggatat taaattgcat aatgacgggt
      721 aactatctgt tgtcagtaag attaccccta tgttgctaca gagacatcaa tgtttcaacg
      781 accagaagat cttttctgcc agaccttgcg aaatacccgg cacttttgca atttcctcga
      841 cgctggcgtt acgtaaacct tgcaaaccgc ccatatattt caacaacatt tgccggcgtt
      901 ttggcccgac gccttcaatg gtttccaggg aactggtatt tttgaccttc gcccgttttt
      961 tacggtgccc gccaatcgcg tgatcatgtg attcatcgcg aatatgctgg ataacatgca
     1021 gcgcgggaga atctggcggc aaactgaatc cctcaccttc cggctcaaag aacagcgttt
     1081 ccagcccagc cttacgatct gctcctttgg caacgccaag tagcagcgga tgatttttat
     1141 cccatgagac atccagttcg gcgaagacat ttttcgcctg cgcaagctgg cctttgccgc
     1201 cgtcgataag tatcacatcc gggatcttac tgtcgtcgat ggctttacca taacgccgac
     1261 gcagcacctg attcatcgcc gcataatcat cgcccggcgt gatgccagta atgttatagc
     1321 gccgatactc cgcacgcagc gggccgttag catcaaacac cacacaggaa gcgacggttt
     1381 gttcgcccat ggtatggctg atgtcaaagc actccatccg cttcacttcc ggcaatttca
     1441 acacgctggc aagcgcggtc agtcgctggt gaacggtaga ttgctgcgaa agtttgctgg
     1501 ttaaggccgt cgccgcattg gtgcgcgcga gtttcagata acgcgcccta tcgccgcgag
     1561 gtttggtttg aacattaatc ttgcgtcccg ccagttctga aagggaatcg gcgagcagcg
     1621 ttttatcgct aagattaaaa tcgagcagga tctcacccgg taaggtgcgc atctggctgc
     1681 cttgtaaata gaactggcct acgaaggttt ctaccacctc gctcagttcc gtaccgccag
     1741 gcactttcgg gaaatagctg cggctgccga gcactttgcc ctgacgaatg aacaatacgt
     1801 ggacacaagc catgcccgca tcgaacgcca caccaataac gtcgaggtcg tcgccggtat
     1861 tggaaacaaa ctgtttttcg gtgacgcgtc gcaccgcctg aatttggtcg cgaatacgtg
     1921 cagcttcttc aaactccaga ttctggctgg cagtttccat acgactaatg agttgcgtaa
     1981 gcacctgatc atctttgcca gacaaaaaca ggcgcacata ctcgacctgc tgagcgtatt
     2041 cttcttcact caccagtcct tcaacgcacg gtcccagaca gcgccctatc tggtattgca
     2101 gacacggacg cgagcgattg cgataaacac tattttcgca ctggcgaatg gggaaaatct
     2161 tttgtagtag cgccagtgtt tcacgtacgg catagccatt cgggaacggg ccgaaatatt
     2221 cacctttggc atgcttcgca ccacgatgca tcgccagacg cgggtgggta tcaccactca
     2281 ggaagataaa aggatatgat ttatcatcgc gtagcaaaac gttgtaacgc ggctgataga
     2341 gtttgatgta gttgtgttcc agcaacagcg cttcggtttc tgtgtgagta accgttacat
     2401 caatttgctg gatctgggcg accagcgctt cggttttgcg cgaagcgagg ttgctacgga
     2461 aatagctgga aagccgtttt ttcaggtctt tcgctttgcc gacatagata accgtaccac
     2521 cagcatcgta catgcgataa acgcctggct ggctggttac ggtttttaaa aacgcttttg
     2581 cgtcaaactg atcactcact gacttgataa tgtctccgca ttacacagac catggcgaat
     2641 tgccaggtga gtcagctcaa catcgccatg aatgtttagt ttactgaaca tacgatagcg
     2701 gtagctgttc accgttttcg gactgagatt gagctgttct gagatctcat tgaccttctg
     2761 gcccttggtg atcatcagca taatctgcaa ttcacgttca gacaaactgg caaatgggct
     2821 ttctgttttt tctggttcga tctggcttaa cgccatttgt tgagcgatgt cagaagcaat
     2881 gtaacgctgc cctgaataga cagaacgaat cgcactcacg acttcctgcg gagccgcgcc
     2941 tttgctgagg tagcccgcag caccggcctg catgactttc gctggtaaag ggttttctgt
     3001 atggacggta agcatgatga ttttgacatc agctgtggaa cgcgcgattt tacgcgtcgc
     3061 ctcaagaccg ccaatgcccg gcatactcat gtccattagc accacgtcaa cggcatttgt
     3121 ccggcaccac ttaacggcgt cttcaccgca cgatgcctca ccgacgactt ttataccctt
     3181 tatatcttcc agaatgcgtc gtatccctgc gcgcaccagt tcgtggtcat caacaagtag
     3241 aacgttgatc aaaggaatat ctccagaaat agggataacg ctactgatag ttagtcattc
     3301 gtatattagc ggtttttatt gcaactttga aacgttaaaa atggtgggta ttcgattttt
     3361 ctctcgtttt tgtcaattcg attgtccaca gttatggtca cgcccgtcac caacggctta
     3421 ccggatcgtt tcacagtaag ccctacgatg aaatgtgaca aaaattgact ttattcagca
     3481 aaaatgaaaa tcagcccgct gaatatgtaa cattaattaa cccgaacaat acgggcaaaa
     3541 aacattcatg ataaaaatat ttattggtca ttatattaac gtattttata gcactgccga
     3601 tatcacgctc aaaaaacaac cactgctatt tttagcaaag cttatggtat actccgccgc
     3661 cttaacattt ttcaccgcaa attttcattg caacatgacg aggaaaataa atgagtacgc
     3721 ctgatttttc tactgccgag aataatcaag aactggcaaa tgaagtctcc tgcctgaaag
     3781 cgatgctgac gctgatgctg caggcgatgg gacaagctga cgcgggccgc gtgatgttaa
     3841 agatggaaaa acagcttgcg ctgatcgaag acgaaaccca ggctgcagta ttttccaaaa
     3901 cggttaagca aattaaacaa gcctaccgtc agtaatataa aaccggctga gagcgtgcct
     3961 ttcagccggt ttttgcatct ggcacgcaga aagaaaagcg atcaaattaa gccagtagcg
     4021 gccgcgtaac aggccacctg ggtcttattt ggtgcattga tttttttctg catgtttttc
     4081 tgatggaaat tgaccgtatt ctcagagatt gacaaaatca tcgctatctc tgctgatgtc
     4141 ttcccttccg ccgtccattt cagaatttct ttttcgcgct tgctgaaatt catctctggc
     4201 gtcatcacta tttcatcatt taaacgcatc agagccatca gactttcgcg caccagtaac
     4261 tgcattttta attgcagttc atcactaaga atgggtattt cgcgcgtgct gcaacgggaa
     4321 aaggacaaaa agcccagcgc ccgatttggc agcattaaat actgagtgac accgcggcgt
     4381 aaaccatgtg cgcgcgcggc ttcccataac ggctgtgctt cgctgaataa gtcatcattc
     4441 cacattaaat ggccctgact aaagttttca gggttcagca ccggatcaat tgcgagaaag
     4501 ttttttgcct gataataact aacccacgcc tcagggtaat tggtgtagaa agccacttta
     4561 ggtcgcgtga atggtaccgg gtggcgaaca cataacgaat agtaatcgta ctccagctgc
     4621 tgagcctgaa gctctatttc atggtagacc tcttctgcgg cctccatcct ctgaaaacgc
     4681 aacagcatcg tgcgacgcca gctgaaaaaa tccgtatcct gcatagtaaa ccgcaacgcc
     4741 cctgagagtg agtatcattt ataatgataa ttcaaactat cacataaata agatttatat
     4801 ataatttata ttattcaggc aatgaattac ttttgcaagc catcgcattc tcttatgtta
     4861 ttaatgagtt atgctgattt gttaagcagt tttatcacgc ttgaaatggc gtcccgcccc
     4921 gacaggtgaa tcgtcggggc tgattttttc ttattattgc agcagaaact tctcgaggaa
     4981 ctggcgggtg cgaggctgct ggggatcagc aaataacgct tttgctgccc cctgctcgac
     5041 tatccgcccc tggtccataa aaatcgcccg gtccgcaaca tcccgggcaa agctcatttc
     5101 gtgcgtcaca atcaccatcg tgcgcttttc ctgcgccagc tggcggattg tgttcaggac
     5161 ttcgcccacc agctctggat ccagcgctga cgttggctcg tcaaacaaaa tcacctcagg
     5221 acgcattgcc agcgcacgcg caatcgcaac acgctgctgt tgaccgccag acaaacgacg
     5281 tggatagctg gtttctttac cggccagccc aactttcgcc agcagctcgc gagcgcgcgc
     5341 cgtggcctct tctttcggtt cgcctttgac gatcaccggc ccttcaataa tgttttccag
     5401 caccgtacga tgcggaaaca aattaaagtt ctggaagaca aacccgacgt gctgacgcaa
     5461 ctggcgaatc agcgattttt gctgacttaa tgaacgtgca gtatcaatag tgatatcgcc
     5521 aacggtgatc gttcccgctt ccggttgttc cagcagattt atgctgcgta gcaacgtggt
     5581 tttgccggaa ccactcggac cgataattgc caccacttcg ccaggcttta cctcgaggtc
     5641 gataccgtgc agcaccgtct gaccgtgaaa ttttttcacc aggttcttaa cttcaatggc
     5701 actcattttg gttctctctc ctggcgatta agttgattct caaaatggtt ctgcaacgtc
     5761 gataatactg tcgccatgat ccagtagatg agcgaagccg ccaggtacat ggtgaacacc
     5821 tccagcgtgc gcgaggtaat caactgcgct tgacggaaca gctccggcac ctggattgtc
     5881 gcggccagcg acgtatcttt taccaggctg ataaagctgt tcgacagcgg cggcaacgcc
     5941 acccgcgccg cctgtggcaa aatcgcgcga cgcatggtct gccacggcgt cataccaata
     6001 ctggcagcgg cttcccactg acctttatca atagaagaaa tggcggcgcg cagcgtttcg
     6061 gcagcatagg cggcagtgtt cagcgacaga ccaatcattg ctgacggaat gggatctaat
     6121 tcgataccaa actgcggcag gccgtaatag atcataaaca gttgtgcgat gagcggtgta
     6181 ccacgaaaaa tagagatata aaaacgcgcc agccagcgca cgggccagat tagcgacaga
     6241 cgcatcagcg cgagaataaa ccctagcagt aagccaaaaa acatgccgcc gatactgagt
     6301 tgcagggtat acccagcccc tttcaacagg aacggcaagg aatcaataac cagttgtata
     6361 ctttcttgca tgagcgttcc cggagctaaa cgtggggatg ataggcgaac agcgcaggcg
     6421 cgccgccggt atgaataaac agaatcggcc cttcatcttt gaagcgtttc tgactgatac
     6481 cgtcaatcag ccccgccatc gcttttccgg tatacacagg atcaagcaga atgccttcca
     6541 gacgcgccag caatttcact gcttccatgc cttcgtcgtt cggcacgccg tagccaggtg
     6601 caaaatagtc atcccagagt aaaatttccg ctgatgcggt cagctccagt tctttcgcaa
     6661 tcgcctgttg taggttaacc actttcggca attgatcggc aacggaacgc gacacggtca
     6721 cgccaatcag ttcgctttca ggcatcaggt gttccagccc aacagccaat ccggcgtgag
     6781 ttccggcact gcccgatgcg accaccaccg acgaaatatt aaccgcccct tcacactgtt
     6841 gcgcgatttc cagcgcactc tccacataac ccagcgcgcc cagagcatta gaaccgccaa
     6901 ccggaatgac atacgggcga aagccttgtg cttcgactcg cgtcgccagc tcttccagtt
     6961 gggcattggg atcggtcagt gcgtcgcaca tttcaatctg ggtattgaac agatccagca
     7021 acaaacgatt gccgttggtt aaatagtttt ctgcggttgt gccaatagga ttttccagca
     7081 gcgccacgca gtgcagaccg agtttcgccg cgactgcggc agtctggcgc acatggttag
     7141 actggatcgc gccggcagta atcagcgtat cggcaccttc gcgcagggca tctgccgcga
     7201 gaaattccag cttacgtaat ttattgccgc ccattgccat gggtgtgacg tcatcccgtt
     7261 tgatgaaaat ttcccgtcca agataatcag aaaagcgcgg cagatattcg agcggcgttg
     7321 gcgcgccgat aaactccaac cgtggaaaac gggttaaatt atgcagtggc ataacagcct
     7381 ccgatgtgtg ttgttgtgat tttcttatta tgcacgctga aaacgcgtaa ataaaaaagg
     7441 cgctagtgaa agcgcccttt tttgtcatta tgctgattat ttggtcacat cagcaccaaa
     7501 ccatttttcg gaaagagctt gcagagtgcc gtctttttgc atttccgcaa ttgcatcatt
     7561 caccgctttc agcaggtctt catttccttt acgcagcgcc acgccagact cctgacggga
     7621 gaatgcttca ccggttactg ccagcgtatc gttggttttc ttcaccagat ccagcgccgc
     7681 cagacgatca acgaggatcg catcgatacg gcctacgcgc agatcctgat atttggtcgg
     7741 gtcatcatca taggtacgca catcgacgcc ctgaacattc tgccgcagcc actcttcata
     7801 gttggtgccc agaccgacac ccactttttt gcctttcaga tcagcagccg ttttaatggt
     7861 gccttcgtta ccttttttca ccagcgcctg aataccggaa atggtgtaag gggtagagaa
     7921 atcgtatttt ttcttgcgct cgtcagaaat ggtgacctga ttaatcacca catcaatgcg
     7981 tttagagtcc agcgacgcca gcataccgtc ccatttggtc ggttttagtg acgcctcaac
     8041 gccaagatgt tttgccagct gttgggcaaa ttccacttca aaaccggtta atttgccgtc
     8101 atctccctga aaactgaacg gcggataagt gccttccagc cctaccagca gcgtgccgcg
     8161 ctctttaact ttattaagca gaccttcatc tgcaaaactt ttaacgctca tgcccgcaac
     8221 cagcgctacg gccatcacac ccatcaatgc ctgacgtccc agatgtgcta atttcatatt
     8281 taccccgaat gttgttatgt ctgtttgcag tgtagagcca ttgtttgtaa acacaaaaac
     8341 aactccgcta catcttattc ttatttaata tatatcagaa gcaggcaggc tggaggattt
     8401 ctgcacaagt ccggtacacg tttgacgctg atagtgttga tacttccgta atgcgatgcg
     8461 gtaattgttg gtgctggtgg tgggcagcca cggggcgagg atttcatcaa ggaaaccgtc
     8521 ctgcagcgga tcgagggaaa tattttgctc gtgtagatga ttccccaaac ggcgcagacg
     8581 aacgacatat tcacgcaccg taccagggct catttcagtt tgctcaaaaa gaaattgctt
     8641 aaagccgata atatcgaaaa aatcactctg cgttttgcaa tgtaaatcac cgcagaatcg
     8701 gcacaatgcc gcccatgatt tttgttccac ttgccagcca ttttcatcaa gcagcatgtc
     8761 caggcgggaa atctcgattt tattcactat tttgccgtcg cgaacgaggg taatgcggtc
     8821 gagtaattta cggcaatgcg cgcaatgggt ctggctgtgt ttaaagtctt taagatagcg
     8881 gcttaatggc cgtcttttca ggtgctgcac catcattaag aactcctggt agtcaaaatt
     8941 aaagtgcggc atttactgac gttataactt acccagttta gtgcgtaacc gtttaatggc
     9001 ctggctgtgt aactgactga cccgcgattc cccgacctcc agcaccgcgc caatctcttt
     9061 gagattcagc tcttcctggt aatagagggt taataccagt ttttcgcgct ccggcaacgt
     9121 ttcgatagct tccatcactc gctggcgcag attactgtcc agcagttgtt gtagcgggtt
     9181 ttctcgctga tgatcatcag taaccagttc gatgctatcg ccgtgctctt cgcgccactc
     9241 atcgtaggag aagagctggc tgttattggt gtcgagcaac atttggcgat aatcggcaat
     9301 atcgatccct aaacgttccg ctacctcagt ttccgtggcg ttgcggccaa gttcctgctc
     9361 cagttgccct attgcctgtg ccacttcacg cgcgttgcgt cgcacgctgc gcggcaccca
     9421 gtcacggctg cgaagttcat ccagcatagc gccacggata cgctgcactg cgtaagttgt
     9481 aaatgccgtt ccttgtaggg cgtcatagcg ttcgacggca ttaagtaacc caatgccgcc
     9541 cgcctgtagc agatcgtcaa gttccacgct cgcgggcagt cgaacctgca ggcgcaatgc
     9601 ttcgtgacgc accagcggga cataacgctg ccacagcgag tgtttatcca ttacaccttc
     9661 agcggtatag agtgaattca cgataaacag ccctgcgtta tatgagttat cggcatgatt
     9721 atccgtttct gcagggtttt taatcggacg attagtgggt gaaatgaggg gttatttggg
     9781 ggttaccggt aaattgcgga cagaaaaaaa ccccgccggt ggcggggaag cacgttgctg
     9841 acaaattacc attcatgttg ccggatgcgg cgtaaacgcc ttatccggcc tacaaaaatg
     9901 tgcaaattca ataaattgca gttcaccttg taggcctgat aagcgcagcg catcaggcaa
     9961 tttggcgttg ccgtcagtct cagttaatca ggttacgacg attaaccctg cagcagagac
    10021 agaacctgct gcggtacctg gttagctttt gccagcacgg agttaccggc ctgctggatg
    10081 atctgcgctt tcgacatgtt ggacacttcg gtcgcatagt cggcgtcctg aatacgggac
    10141 tgcgcttcgg acaggttggt agtggtgttg ttcaggttgg tgaccgcgga atccagacgg
    10201 ttttgcaccg caccgaggga ggagcggaat ttgtcgatgg agctgatggc ttcgtccaga
    10261 gctttcagtg gatcggcggt ggttgcagct ttagtttcag catctgtcgt caatttaccg
    10321 tcagcatctt tataaactgc cttaccgctg ccgttagtca ctgaaccatc atcctgtaga
    10381 taaaaatcta cagtatcaga tgtggtcaac gccccgttgt tatcaatata aaccggagca
    10441 acaccttttg ttacagcgcc agttgcgccg tcgacgctat aacctgcggt agtgaaatca
    10501 acatcgtttc cagaggttga tacagcttta tcgaaagctg cctgatcaac ttttgcacct
    10561 gttactgaat aagtaatagt gttcggcgta gtactattac cggtaaagga gatagaggta
    10621 ccgccaatgt caatggttgt cgctttggta ttattggctg caagtgtgga cagactagcc
    10681 tcagtcaaag aagcactaaa gccgtttttc gttaagcgcc cagttgtatc aatgtaaagt
    10741 ttatctccat tgcttgacgt aattttacca tcactgctga tctgaacatc ctgtgcagaa
    10801 ccatcgatag taatagtgcc cttagcggta tcaccaacgc ccggggtcaa atatttctga
    10861 acatcggcag ctgaagccgt tgtggtatca aaagagtaag acttacttgc actgtcatac
    10921 ttgtaattcg ttgcactagc cgccgttccg aagccgttat taatggttgc ctgaacagta
    10981 tcaccatcac taactccagc cagcaaatca gacgctttag cagcatcgta acccgcactc
    11041 acagtatatt tgttgcctac cacagttgca ttagcagcta ccaagtcagc tttagatgca
    11101 gcagtgttag caacagcccc gccaccattc acattaaacc cactcagccc cagcgtatct
    11161 gagtcaattt tcttcagatc aatcgtgata gtctggccgt cattcgcacc aacctgaatt
    11221 ttcatggaac cgtctttcgc cagtacgttc acgccgttga actgggtctg gccagatacg
    11281 cggtcaattt cgtccagacg ggatttgatt tcgtcctgaa tggagtccag atccgaatcg
    11341 gagttagtcc cggtagaagc ctgaaccgtc agttcacgga tacgctgtaa gttgttgttg
    11401 atttcggaca gcgcgccttc ggtggtctgc gcaacggaaa taccgtcgtt ggcgttacgt
    11461 gcagcctgag tcaggccttt aatgttagaa gtaaaacggt tagcaatcgc ctgacctgcg
    11521 gcgtcatcct tcgcgctgtt aatacgcaag ccagaagaca gacgctcgat agaactcgac
    11581 agcgcagact ggttcttgtt gatattattt tgagtgatca gcgagaggct gttggtatta
    11641 atgacttgtg ccatgattcg ttatcctata ttgcaagtcg ttgattacgt attgggtttc
    11701 cacccgtcgg ctcaatcgcc gtcaaccctg ttatcgtctg tcgtaaaaca acctttagaa
    11761 tttttttcac aaacagccat tttttgttag tcgccgaaat actcttttct ctgcccctta
    11821 ttcccgctat taaaaaaaac aattaaacgt aaactttgcg caattcaggc cgataacccc
    11881 ggtattcgtt ttacgtgtcg aaagataaaa ggaaatcgca tggcaagtat ttcatcgctg
    11941 ggagtcgggt caggtctgga tttaagttca atccttgata gcctcaccgc cgcgcaaaaa
    12001 gcgacgctaa cccccatttc aaatcagcaa tcgtcgttta ccgctaaact tagcgcctac
    12061 ggtacgctga aaagcgcgct gacgactttc cagaccgcca atactgcatt gtctaaagcc
    12121 gatctttttt ccgccaccag caccaccagc agcaccaccg cgttcagtgc caccactgcg
    12181 ggaaacgcta tcgccgggaa atacaccatc agcgtcaccc atctggcgca ggcgcaaacc
    12241 ctgaccacgc gcaccaccag agacgatacg aaaacggcga tcgccaccag cgacagtaaa
    12301 ctcaccattc aacaaggcgg cgacaaagat ccgattacca ttgatatcag cgcggctaac
    12361 tcgtcgttaa gcgggatccg tgatgccatc aacaacgcaa aagcgggcgt aagtgcgagc
    12421 atcattaacg tgggtaacgg tgaatatcgt ctgtcagtca catcaaatga caccggcctt
    12481 gataatgcga tgacactctc ggtcagcggt gatgatgcgc tacaaagttt tatgggctat
    12541 gacgccagtg ccagcagcaa cggtatggag gtctcggttg ccgcccagaa tgcgcagctg
    12601 acggtcaaca acgtcgccat cgagaacagc agcaacacca tcagcgacgc gctggaaaac
    12661 atcaccctga acctgaacga tgtcaccacg ggcaaccaga cgctaaccat cactcaggac
    12721 acctccaaag cgcaaacggc gattaaagat tgggtgaatg cctataactc gctaatagat
    12781 accttcagca gcctgaccaa atacaccgcc gtagatgcgg gagctgatag ccagagttct
    12841 agcaatggcg cactgctcgg cgactccacg ttgcggacga ttcagacgca gttgaaatcg
    12901 atgctgagta ataccatcag ttcttccaac tataaaacgc tggcgcagat tggtatcacg
    12961 accgatccca gcgatggcaa actggaactg gatgccgaca aactcaccgc tgcactgaaa
    13021 aaagatgcca gcggcgtagg tgcattgatt gttggcgatg gtaaaaaaac cggcatcacg
    13081 accaccatcg gcagcaacct gaccagttgg ctttcgacaa cgggcattat taaagccgct
    13141 accgatggcg ttagtaaaac cctgaataag ttaactaaag actacaacgc cgccagcgat
    13201 cgcattgatg cgcaggtcgc gcgctataaa gaacaattta cccaactgga cgttttaatg
    13261 acctcgttaa acagcacaag cagctactta acgcagcagt tcgaaaacaa cagtaattcc
    13321 aaataagcaa tattcatcgg gagacaggtc atgtacgcgg caaaaggcac ccaggcctat
    13381 gcacaaattg gcgtcgaaag cgccgtaatg agcgccagcc agcagcagct ggtcaccatg
    13441 ctatttgatg gagtgctgag cgcactggtt agagcgcgcc tgtttatgca ggacaacaat
    13501 cagcaaggca aaggcgtctc tttgtcaaaa gcgatcaaca tcattgagaa cggactgcgg
    13561 gtgagtcttg atgaagagag caaagacgaa ttaacccaaa acttgattgc tctttatagc
    13621 tatatggtca ggcgtttgct gcaagccaat ttacgcaacg atgtctccgc agtcgaagaa
    13681 gtggaagcat taatgcgcaa tattgccgat gcctggaaag agtcgttact ctccccttct
    13741 ttgattcagg acccagtctg atgaaccatg caccgcattt gtatttcgcc tggcaacaac
    13801 tcgtcgaaaa aagccagctc atgttacgcc tggcaacgga agaacaatgg gacgaactca
    13861 tcgccagcga aatggcgtat gtgaatgcgg tgcaggagat tgcacatctg actgaagaga
    13921 ttaacccatc caccacgatg caggagcagc tccgcccgat gctgcgcctg attctcgaca
    13981 acgaaagcaa ggtaaagcag ttattacaga ttcggatgga tgaactggcg aaactggtcg
    14041 gtcagtcatc ggtgcaaaaa tcggtgttaa gtgcctatgg cgatcagggc ggctttgtgc
    14101 tggctccgca agataacttc tcgtgaatct taacaagcta atggctcgcg aataatctga
    14161 ttacggctac gcttctaatg ttccccttga atggagtcga agaatgcgta atcccacgct
    14221 gttacaatgt tttcactggt attacccgga aggcggtaag ctctggcctg aactggccga
    14281 gcgcgccgac ggttttaatg atattggtat caatatggtc tggttgccgc ccgcctataa
    14341 aggcgcatcg ggcgggtatt cggtcggcta cgactcctat gatttatttg atttaggcga
    14401 gtttgatcag aaaggcagca tccccactaa atatggcgat aaagcacaac tgctggccgc
    14461 cattgatgct ctgaaacgta atgacattgc ggtgctgttg gacgtggtag tcaaccacaa
    14521 aatgggcgcg gatgaaaaag aagctattcg cgtgcagcgt gtaaatgctg atgaccgtac
    14581 gcaaattgac gaagaaatca ttgagtgtga aggctggacg cgttacacct tccccgcgcg
    14641 tgccgggcaa tactcgaagt ttatctggga tttcaaatgt tttagcggta tcgaccatat
    14701 cgaaaaccct gatgaagatg gcatatttaa aattgttaac gactacaccg gcgaaggctg
    14761 gaacgatcag gttgatgatg aattaggtaa tttcgattat ctgatgggcg agaatatcga
    14821 ttttcgcaat catgccgtga cggaagagat taaatactgg gcgcgctggg tgatggaaca
    14881 aacgcaatgc gacggttttc gtcttgatgc ggtcaaacat attccggcgt ggttttataa
    14941 agagtggatc gaacacgtac aggaagttgc gccaaagccg ctgtttattg tggcggagta
    15001 ctggtcgcat gaagtcgata aactgcaaac gtatattgat caggtggaag gcaaaaccat
    15061 gctgttcgat gcgccgctgc agatgaaatt ccatgaagca tcgcgcatgg ggcgtgacta
    15121 cgacatgacg cagattttca cgggtacatt agtggaagcc gatcctttcc acgccgtgac
    15181 gctcgttgcc aatcacgaca cccaaccgtt gcaagccctc gaagcgccgg tcgaaccgtg
    15241 gtttaaaccg ctggcatatg ccttaatttt gttgcgggaa aatggcgttc cttcggtatt
    15301 ctatccggac ctctacggtg cgcattacga agatgtcggc ggtgacgggc aaacctatcc
    15361 gatagatatg ccaataatcg aacagcttga tgagttaatt ctcgcccgtc agcgtttcgc
    15421 ccacggtgta cagacattat ttttcgacca tccgaactgc attgccttta gccgcagtgg
    15481 cactgacgaa tatcccggct gcgtggtggt catgtcgaac ggggatgatg gcgaaaaaac
    15541 cattaatctg ggagagaatt acggcaataa aacctggcgt gattttctgg ggaaccggca
    15601 agagagcgta gtaaccgacg aaaacggtga agcgaccttc ttttgcaacg gcggcagcgt
    15661 cagcgtgtgg gttatcgaag aggtgattta aattcatccc cggcggcaag ccggggagat
    15721 ttcattacgg cagtggcgtc ggtaacggcg ctttatccag cgcggcggca cactctgccg
    15781 tcgggcgatc cacacgctcc atcgtcatac catcgtactc aatggtgtta ccgtcacgct
    15841 caacctcgta cagctcacgt tttacggtaa cgttagtgag atcatctgac atcaatgtca
    15901 acttgcccgg cacggcaatc acccgctgcc actgacggca atccagggta tcgccctctt
    15961 tagtgacaat caggctgcct atcgcttccg gactgaccag cgcacgttgc ggccctttgg
    16021 tctgccagta gcccgccagc cagtccggcg cgggggtttt cacgacattg ttatagtttt
    16081 cgacttcggc acagcccgcc agcaacatga gcgcgcctgc aattgcgagt tttttcatca
    16141 ttcgtcctgc atgagaagaa aagaggattg tggcattaaa gccctgatgt cgccagcctt
    16201 tccaggttca gagagaaatt tgatctaact attatttttg tgaattatta ccccgaaaat
    16261 aaatttgcat gatgtatgat tcgcgactct tgtttcaaac cttctgagtt cagaggctac
    16321 attcatgtca tggcagcaat tcaaacacgc ctggttgatt aaattctggg cacccatccc
    16381 tgcggtcatc gcggcgggta ttctctccac ttactatttt ggcattactg gcaccttttg
    16441 ggctgtcacg ggtgaattta cccgttgggg cggccagctc ctgcagctgt ttggcgtcca
    16501 tgctgaagag tggggttatt ttaaaattat ccatctggaa ggatcgccat taacccgcat
    16561 cgacgggatg atgatcctcg gcatgtttgg cggctgcttt gccgcagcgc tgtgggccaa
    16621 caatgtcaaa ctgcgcatgc cacgcagccg tatccgcatt atgcaggcca ttattggcgg
    16681 tattatcgct ggttttggcg cgcgtctggc aatgggctgt aacctggcgg cgttctttac
    16741 cggtattcct cagttctcgc tgcatgcctg gttctttgcc atcgccactg ccattggttc
    16801 atggtttggc gcgcgcttta ccctgctgcc catcttccgt attcccgtga aaatgcagaa
    16861 agtttctgcc gcctcaccgc tgacgcaaaa accggatcag gcgcggcgtc gttttcgtct
    16921 cgggatgctg gtctttttcg gcatgctggg ctgggcgctg ctcacggcga tgaaccaacc
    16981 caaactgggg ctggcaatgc tgtttggcgt cggctttggt ttactgattg aacgtgcgca
    17041 aatctgcttt acttcagcgt tccgcgatat gtggatcacc ggacgtaccc atatggcgaa
    17101 agcaatcatt attggtatgg cggtgagtgc catcgggatc ttcagttacg tacagttagg
    17161 cgttgaaccc aaaatcatgt gggcgggacc aaacgcggta attggtggtt tactgtttgg
    17221 ttttggcatc gtgctggcag gcggctgcga aaccggctgg atgtaccgcg cggtagaagg
    17281 ccaggtgcac tactggtggg tcggtctggg caacgtgatc ggctcaacga ttctggcgta
    17341 ttactgggat gatttcgctc cggcgctggc caccgactgg gacaaaatca acctgctgaa
    17401 aacctttggc ccgatgggtg gcctgctggt gacatatttg ctgttgttta ctgcgctgat
    17461 gttgattatc ggctgggaaa aacgcttctt ccgccgtgcg gcaccgcaga ctgctaagga
    17521 gatcgcatga aaaatatcgt tcctgattac cgccttgata tggtgggtga accctgccct
    17581 tatccggcag tcgcaactct tgaggcgatg ccgcagttaa aaaaagggga aatcctggaa
    17641 gtggtgagcg actgtccaca gtcgatcaat aatattccac tggatgcgcg taatcacggc
    17701 tatacggtgc tggatattca gcaagacggg ccgaccattc gttatttaat tcagaagtaa
    17761 tccctcattc ccgctgtggc ccacagcggg aagatcgtta cctttgtatc acatagattt
    17821 tacccgcccc ctctcctatt cttttcagta tgatctcagg aggtttctat gtgtggacgc
    17881 tttgcccaat cccaaacgcg tgtagattac cttgcgcttc tcgcggaaga tattgaacgc
    17941 gatattccct atgatcccga acccattggc agatacaacg tcgcgccggg aaccaaagtc
    18001 cttctgctca gtgaacgtga cgaacacctt catctggatc cggttttctg gggatatgct
    18061 cccggatggt gggataaacc gccactgatt aacgcccgcg tagaaactgc cgcaaccagt
    18121 cgcatgttta aaccgctctg gcaacatggt cgggcaatct gttttgccga tggctggttt
    18181 gagtggaaaa aagaaggcga caaaaaacag ccttatttta tctatcgcgc tgatggacaa
    18241 cctgttttta tggccgcgat aggtagcaca ccgtttgagc gtggtgacga agccgaagga
    18301 tttttaattg tcactgctgc agcagaccag ggtctggtag acattcatga ccgccgccca
    18361 ctggtactgt cgccagaagc tgcgcgggaa tggatgcggc aagagattgg cggtaaagaa
    18421 gcatcagaaa ttgctaccaa cggctgtgtg ccagcaaacc agttcacctg gcacccggta
    18481 tcgcgcgcgg taggtaatgt caaaaaccag ggcgcggagt taattcaacc tgttt
//
LOCUS       DAZFQD010000104        18396 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.104, whole genome shotgun sequence.
ACCESSION   DAZFQD010000104 DAZFQD010000000
VERSION     DAZFQD010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18396
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.104"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..586
                     /locus_tag="ACHKTW_003578"
                     /pseudo
     CDS             1..586
                     /locus_tag="ACHKTW_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309545.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
     gene            702..806
                     /locus_tag="ACHKTW_003579"
     CDS             702..806
                     /locus_tag="ACHKTW_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6464525.1"
                     /translation="MSDDISLAMEGALAVIVVVGVYCLVVLLMDRLGS"
     gene            complement(972..1616)
                     /locus_tag="ACHKTW_003580"
     CDS             complement(972..1616)
                     /locus_tag="ACHKTW_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HFO6464526.1"
                     /translation="MGDSVTPEMDVLSNMIRQYFSREQPEEESIRALNHLRRVLHEVS
                     PFAQEPVDCVLWVKADEVVANDYNPNVMAPSEKKLLKQSLEKDGFTQPVVVSEEKNHY
                     LVVDGFHRQLLGRESVTGKRLRGWLPVACINPERKGQAARIAATIRHNRARGKHQIDS
                     VSDIVRDLSRLGWADERIGTELGMDQDEVLRLKQISGLTELFQEVDFSPAWTVK"
     gene            complement(1601..2827)
                     /locus_tag="ACHKTW_003581"
     CDS             complement(1601..2827)
                     /locus_tag="ACHKTW_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HFO6464527.1"
                     /translation="MSGTITKISTGENVLSAAIHRIEWLFETFNEVCLSFSGGKDSTV
                     LFHLVAEVASRKKRRFSVLFIDWEAQYQCTINHIQKMREMYRHVTDNFYWVALPLTTV
                     NGVSQFQPEWICWEPGVEWVRQPPDDAITDMSYFPFYRYAMTFEEFVPAFSSWFAGNR
                     CGVGILTGVRADESLNRFMGLVSQRKLRYADDKPWTTASPDGFYYTMYPLYDWKTNDI
                     WTYNSRTRAIYNPLYDLMYRAGVSLRNMRVCEPFGPEQRKGLWLYHVLEPETWARMCE
                     RVSGAASGALYANESGAYFALRKRITKPAHHTWRSYAMFMLDVMPERTAEHYRNKIAV
                     YLRWYQTRGFPDDIPDEQENDLGSRDIPSWRRICKTLIKNDFWCRTLSFSPNKPRHYE
                     RYLQRMKERRKEWGIL"
     gene            3000..3272
                     /locus_tag="ACHKTW_003582"
     CDS             3000..3272
                     /locus_tag="ACHKTW_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6464528.1"
                     /translation="MAQHIKSHNSEAVPAIKRGRHSHVFKTHWYQHPPCTEEQAEWLI
                     QCYRRRGYEVKKALSLNYRHWIISVRLPYSERPPRPSRTFQQRIWR"
     gene            3274..4323
                     /locus_tag="ACHKTW_003583"
     CDS             3274..4323
                     /locus_tag="ACHKTW_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFO6464529.1"
                     /translation="MRVLLRPVLVPELGLVVLKPGRESLPVFHCGRVLVEPEPKSMRA
                     LPSGFVPAVRQPLAEDKSLLPFFSDERVIRAAGGAGVLSDWLLRHVKSCQWPHGDYHH
                     SETVIHRYSTGAMVLCWHCDNHLREQTSDSLELLAQQNLAAWMIDVIRHVMNGAQERE
                     LSLAELSWWAVCNQVADALPESVLRRSLGLPVEKIRSVYSESDIIPGEQTATSILKQR
                     TKNIAPPLHAHQPQTTPQEKTVVSIAVDPESPESFMKRPKRRRWVNEKFTRWVKTQPC
                     ACCGKPADDPHHLIGHGQGGMGTKSHDIFTLPLCREHHNELHADPLEFEKKYGSQVEL
                     IFHFLDHAFATGVLG"
     gene            4336..4695
                     /locus_tag="ACHKTW_003584"
     CDS             4336..4695
                     /locus_tag="ACHKTW_003584"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFO6464530.1"
                     /translation="MLIDLVLPYPPTVNTYWRRRGSTYFISEEGKRYRRAVALIVNQQ
                     RLKLSLSGRLVIRIIAEPPDKRRRDLDNILKAPLDALTHAGLLMDDEQFDEINIVRGL
                     PVPGGRLGIKITELECA"
     gene            4692..5375
                     /locus_tag="ACHKTW_003585"
     CDS             4692..5375
                     /locus_tag="ACHKTW_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001064904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage antitermination protein Q"
                     /protein_id="HFO6464531.1"
                     /translation="MNNQYLQFVREQLMIATADLSGATKGQLEAWQENALFDTGRYRR
                     KKIRYRDEVTGKMITRDNQPIPGKQSLAKGSSIALVSPVEFATSSWRRAVLELEEHQK
                     AWLLWCYSGNVCWEDQVTITQWVWGEFKAQAGTRKIAGKTLDRLKKLIWLAAQDVKSE
                     LAGRETYEHQELAVFIGVAKSTWTKTHRPHWLAMRGSAEMLDRDALISVMRVRSRQKS
                     INLEPYLAK"
     gene            5818..6015
                     /locus_tag="ACHKTW_003586"
     CDS             5818..6015
                     /locus_tag="ACHKTW_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HFO6464532.1"
                     /translation="MLKQQDMTETARVVFNELSVTEPATVGEIAQNTYLSRERCQLIL
                     TQLVMAGLADYQFGCYRRLPQ"
     gene            6166..7224
                     /locus_tag="ACHKTW_003587"
     CDS             6166..7224
                     /locus_tag="ACHKTW_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFO6464533.1"
                     /translation="MLNTVKISSCELINADCLEFIRSLPENSVDLIVTDPPYFKVKPE
                     GWDNQWKGDDDYLKWLDQCLAQFWRVLKPAGSLYLFCGHRLASDIEIMMREHFSVLNH
                     IIWAKPSGRWNGCNKESLRAYFPATERILFAEHYQGPYRPKDAGYEAKGRALKQHVMA
                     PLISYFRDARAALGITAKQIVDATGKKNMVSHWFSASQWQLPDESDYLKLQALFARVA
                     EEKHQRGELEKPHHQLVSTYSELNRQYTELQSEYKHLRRYFGVTVRVPYTDVWTHKPV
                     QYYPGKHPCEKPAEMLQQIISASSRPGDLVADFFMGSGSTVKAAMVLGRRATGVELET
                     ERFEQTVREVQDLASQNG"
     gene            7265..7340
                     /locus_tag="ACHKTW_003588"
     tRNA            7265..7340
                     /locus_tag="ACHKTW_003588"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7298..7300,aa:Ile,seq:cat)
     gene            7350..7426
                     /locus_tag="ACHKTW_003589"
     tRNA            7350..7426
                     /locus_tag="ACHKTW_003589"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7384..7386,aa:Arg,seq:tcg)
     gene            7705..8133
                     /gene="tolA"
                     /locus_tag="ACHKTW_003590"
     CDS             7705..8133
                     /gene="tolA"
                     /locus_tag="ACHKTW_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000271626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HFO6464534.1"
                     /translation="MYCRAVPVLVLILASLTTGCTKDVSTNNYDASLHHSDKLIKSQN
                     LSLSERTPDASEEIRQYAIQVREAIEEQLKDAGKYSGKECSLRMYMAPNGLLLQVKRE
                     SGDPDLCREAMNAVKNADIPSPPSSGVYKAFRNGVLDFKL"
     gene            8600..8764
                     /locus_tag="ACHKTW_003591"
                     /pseudo
     CDS             8600..8764
                     /locus_tag="ACHKTW_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000483513.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
     gene            complement(9260..9345)
                     /locus_tag="ACHKTW_003592"
                     /pseudo
     CDS             complement(9260..9345)
                     /locus_tag="ACHKTW_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309987.2"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            9602..11590
                     /locus_tag="ACHKTW_003593"
     CDS             9602..11590
                     /locus_tag="ACHKTW_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000142995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFO6464535.1"
                     /translation="MAFKHYDVVRAASPSDLAERLTQKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVTTPVLVKPSGDGETAISKTGEPEYYYVVALAGQSNGMSFGEGVPLPDSY
                     DRPDPRIKQLARRRTVTPRGVPCTYNDVIPADHCLHDVMDMSEKNHPRADLNKGQYGC
                     VGQGLHIAKKLLPFIPANAGILLVPCCRGGSGVTTGNEGTFNASSGASPDSALWGADK
                     PLYQDLLSRTKVALAKNPGNILLAVIWMQGESDLASGSQSHNALFTGMVQKFRADIAD
                     MSGQCVGGSALSVPWVCGDTTHYWKEKYAASYETVYGGYKNKAAQNIHFVPFMVDGSG
                     VNVQTNNPAEDPDIEGIGYSGSKWRDSSVSWTSGDRASHFGSWARRGIISDRLATAIL
                     THAGKYPAFITGRSETVVAGDERENVTSGNFPSPVEDAALSQDAPQRRTVVEYNSAVE
                     KDSLGGNWAAQHWNARDGVAEFVNDESRQVLKIKQHEGKQGPWMLSAGVSAEDARALY
                     ENGGEIKLEFRLPDGTQVADDKYLFGVYLPVSGWTQEGNGSNMMASFFTQTKASNLDV
                     MYHGKTQPNTKIESFGAVNNNWHTLVFKYKGGGNHQVIPVLDGEEKTGFALKEGPVTS
                     AFTVDNITVTGISANQGKSYPVMFRSIMVSINQATSSS"
     gene            11737..11919
                     /locus_tag="ACHKTW_003594"
     CDS             11737..11919
                     /locus_tag="ACHKTW_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001304601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HFO6464536.1"
                     /translation="MTFVHQLMLYFCTVVCVLYLLSGGYRVVRDFWRRQIDKRAAEKI
                     SASQSAGSKPEDPLIP"
     gene            11957..12226
                     /locus_tag="ACHKTW_003595"
     CDS             11957..12226
                     /locus_tag="ACHKTW_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HFO6464537.1"
                     /translation="MSEIKSLVTAEAVKEVLRSEEVRSALKQKLRQNLEARLDAEVDA
                     ILDELLGAPTVPEPEGIADDSAVSDGVGSQPDGSEEPQPDGEMMM"
     gene            12301..12516
                     /locus_tag="ACHKTW_003596"
     CDS             12301..12516
                     /locus_tag="ACHKTW_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFO6464538.1"
                     /translation="MYQMEKITTGLSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTYLTNLYFKIKEDRRKAARGE"
     gene            12521..13054
                     /locus_tag="ACHKTW_003597"
     CDS             12521..13054
                     /locus_tag="ACHKTW_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO6464539.1"
                     /translation="MSRKLRYGLSAAVLALIGAGASAPEIFDQFLDEKEGNHTTAYRD
                     GAGIWTICRGATRVDGKPVIPGMKLTKEKCDQVNAIERDKALAWVEKNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRINAGDRRGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     ISRRDQESALACWGIDR"
     gene            13572..13757
                     /locus_tag="ACHKTW_003598"
     CDS             13572..13757
                     /locus_tag="ACHKTW_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1 family lipoprotein"
                     /protein_id="HFO6464540.1"
                     /translation="MRELKMKLCVLMLPLVVSACGSTPPAPVPCVKPPAPPAWIMQPA
                     PDWQTPLNGIISTSENG"
     gene            13843..14067
                     /locus_tag="ACHKTW_003599"
     CDS             13843..14067
                     /locus_tag="ACHKTW_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6464541.1"
                     /translation="MKKLRVTVEPFQGTIPFRILQRGRVLVEGSFSGKCTQLHSRTFQ
                     VNATNEELTVECTMNAAKCRMVSAALQPVC"
     gene            14413..14739
                     /locus_tag="ACHKTW_003600"
     CDS             14413..14739
                     /locus_tag="ACHKTW_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HFO6464542.1"
                     /translation="MKRNLPLIILLSSLVMGCTQHKTDMPRQLVKALPQYPAYAAANY
                     IKGRVDVKFDIGADGTVTRIEFIRSEPHHLFDEQVVKAMAKWRFEKDRPRKGVKKTFI
                     FSPSAP"
     gene            complement(14871..15071)
                     /locus_tag="ACHKTW_003601"
     CDS             complement(14871..15071)
                     /locus_tag="ACHKTW_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFO6464543.1"
                     /translation="MSSKNRTRRTTTRNIRFPNHMIEQINIALEHKGSGNFSAWVIEA
                     CRRRLATDATHLRPASMTNNEK"
     gene            15113..15478
                     /locus_tag="ACHKTW_003602"
     CDS             15113..15478
                     /locus_tag="ACHKTW_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFO6464544.1"
                     /translation="MAQLRTLQSRIKTLNTRRVNILKGEQRRVSGSARVSLKRHIWLR
                     DAGQCCLCGRVVDLCDSELDHRIALQFGGGNEETNLWTLCTECHRQKSVREAASGMPD
                     PTLPEVSGGSGRADDIIGL"
     gene            15768..16331
                     /locus_tag="ACHKTW_003603"
     CDS             15768..16331
                     /locus_tag="ACHKTW_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFO6464545.1"
                     /translation="MLTTQKRKFALALMSGKNKTASAIAAGYSAKTARVKGSQLAKDP
                     EVLAFIARKQGETVEVDEVPVYRQKKTQTEECHQEAPPAEKNTPVSLPPDDRQSLTPL
                     PGIDYMEDGLPDPVKAMGRILVENLSIDPKLALDAAWRLAQFTHHKKGDAGKKSAKGD
                     AAKKAANRFAVPPPPRLVVNNDNEGNG"
     gene            16328..17989
                     /locus_tag="ACHKTW_003604"
     CDS             16328..17989
                     /locus_tag="ACHKTW_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HFO6464546.1"
                     /translation="MIPGWSTACPDWAVRLKKGLSIIPAPIYPEQAAHALAIFKQLRI
                     VDAPGSPTFGESCAQWVFDLVAALFGSYDAQTGVRHIKEVFILIPKKNSKSTLAAGIM
                     MTALLLNWRQAAGYTILAPTVEVAANAFNPARDMVRRDDDLDDLCQVQTHIRTITHRV
                     TDTTLKVVAADPNTVSGIKSVGTLIDELWLFGKQYKAEDMLREAIGGLASRPEGFVVY
                     TTTQSNEPPAGVFRQKLQYARDVRDGKIHDPHFLPVIFEHPPEMVESGEHLLMENLAM
                     VNPNLGYSVDEAFLYREYRKAREAGEETFRGFMSKHANVEIGLALRSDRWAGADFWEQ
                     QGRRISLDDILQRADVVTVGIDGGGLDDLLGMYVIGRDRETREWLGWGHAWAHETAVA
                     RRKSEAARFQDFVACGDMTIVRRVGDDTAEVAEYVRRIHEAELLDHIGIDPSGVGQIL
                     DSLAEAGIPDESVVGISQGWKLGGAIKTTERKLAEGVLVHGGQPLMAWCVGNARVEPR
                     GNAILITKQASGRGKIDPLMALFNAVSLMSLNPEPKKKEYAVFFI"
     gene            18053..>18396
                     /locus_tag="ACHKTW_003605"
     CDS             18053..>18396
                     /locus_tag="ACHKTW_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6464547.1"
                     /translation="MTLKRACSLLTVKSFSEDERVITGIASTPSPDRDGDILEPEGAE
                     FGSAIPFLWQHDHSRPVGQCTVRRVSEGLEITATLAKPVPDMPSQLAARLDEAWAAIK
                     TGLVRGLSVGFRP"
BASE COUNT         4542 a         4223 c         5145 g         4486 t
ORIGIN      
        1 gaccactatt accaaagatt gactgctgac aatccagcat tggcgcgaaa catacggacc
       61 gggtagcaac gttgtgcttc cagcagaaga agcggaagag ctggcacgga ttgcgctggc
      121 atcactggca gcagtatcgg atgaacgagc agcctatgaa ttatttatgg agaagcgttt
      181 cggagaatct gtagatcgcc gcagagcaaa aaatggcgat agagaataca tggcatggga
      241 tatggcgctt ggctggatta tctggtgtca ccgcgccgcc atgcttcagg ctggaaactt
      301 tcgggaaaat aaggggtcgt caaccaataa ttttcgggaa atctcggaaa cgtcaaccaa
      361 ctatccggta actccggatg gttggataag ctgtagtgag cgaatgccgg atgatggtca
      421 gcacgtaatt attttatgtg atggcgcatt cgttctttat gcgcaatatc gagacggtga
      481 gttttttgat gtagtccgta atggtgatga atttttcgaa acacagagcc gcaatgtaac
      541 cggctggatg ccgctaccag aaccgccgca ggaggtgcgc caatgaactg gcctgaagca
      601 tttgcaatta caggcgttgc tatggttatc gcttttttaa tatatgttat ttgtcggtgg
      661 gggtaaaaac gttcgccggg attcacacca aaggagggaa tatgtcggat gatatctcac
      721 tggcaatgga aggcgctctg gccgttattg ttgttgtggg tgtttactgc ctggtcgtgc
      781 ttttgatgga tcgtctgggg agctgaattc attacgatat gggaatttcc atatcgggta
      841 aaaaggggga gtaaaacgag agtcaagtag atgtatcggt cgaaaattga gcagtaaaag
      901 gggcgatatt aatcgccctt gagttgtatt agttatcatt ttgttgcctt gccgggagta
      961 agtcgagctt gctatttaac tgtccatgcc gggctgaaat ccacttcctg aaataactca
     1021 gttagcccgc taatttgttt aagtctcagt acttcatcct gatccatacc caactcagtg
     1081 ccaatgcgtt catctgccca tcccaagcgt gaaagatctc ttacaatatc agacactgag
     1141 tctatctggt gtttccccct agcccggtta tgtcgaatgg ttgcggcaat gcgtgcggct
     1201 tgcccttttc tctctggatt aatacaggct acaggcagcc agcccctcag acgttttcct
     1261 gtaactgatt ctctgcccaa taactgacga tgaaaaccat ccaccaccag ataatgattc
     1321 ttctcctctg acaccaccac cggttgtgtg aaaccatctt tttccagtga ctgcttcagc
     1381 agtttcttct cacttggtgc catgacattg gggttataat cgttggctac cacctcatcc
     1441 gctttcaccc acagcacaca gtctacaggc tcctgtgcaa aaggactgac ttcatgcagt
     1501 accctgcgca gatggttaag ggctcgtatg gactcttctt cgggctgttc ccgactgaag
     1561 tactgacgaa tcatgttgct tagtacatcc atctcaggcg tcacagaatc ccccattctt
     1621 tcctcctttc cttcattcgt tgcagataac gctcatagtg ccggggtttg ttcggactga
     1681 aggagagggt ccggcaccag aagtcattct ttatgagcgt cttacagata cgtcgccagg
     1741 acgggatatc ccggctcccc aggtcattct cctgttcatc cgggatatca tccgggaagc
     1801 cccgcgtctg ataccagcgc aggtagactg caattttgtt acggtaatgt tctgctgttc
     1861 tttctggcat aacatccagc atgaacattg cgtagctgcg ccaggtatga tgagccggtt
     1921 ttgtgattcg tttacgcaga gcaaaatagg caccgctttc attggcataa agagcgccac
     1981 tggcagcccc tgacactcgc tcacacatcc tggcccaggt ttccggctcc agaacatggt
     2041 aaagccacag ccccttacgc tgttccgggc caaagggctc acaaacacgc atgttgcgta
     2101 acgacactcc ggcacggtac attaggtcgt acaggggatt gtagatggcg cgggttcttg
     2161 agttatacgt ccatatatca tttgttttcc agtcatacaa tggatacatg gtgtagtaaa
     2221 aaccatcggg tgaagcggtg gtccagggtt tatcatctgc atatctcagt ttgcgctgag
     2281 acaccagtcc cataaagcga ttgagcgatt catcagcacg aacaccagtt agtattccca
     2341 ctccacatcg gttaccggca aaccaggaag aaaatgccgg aacaaattct tcaaacgtca
     2401 tggcataccg ataaaatggg aaatacgaca tatctgtaat agcgtcatct ggtggctgac
     2461 gaacccattc tacacctggc tcccaacata tccattctgg ctgaaactga gagacgccat
     2521 ttaccgtagt cagaggaagc gctacccagt aaaaattgtc tgtcacatgc cggtacattt
     2581 ccctcatctt ttggatatga ttaatcgtgc actgatattg ggcttcccag tcaatgaata
     2641 aaaccgaaaa acgacgtttt tttctactgg cgacttctgc cacaagatgg aaaagcacag
     2701 tagagtcttt gccaccagaa aaagacaagc agacctcatt aaacgtttcg aacaaccatt
     2761 caatgcgatg gattgctgct gacagaacat tttcccctgt gctgattttt gttatggttc
     2821 ctgacataca atcttctaat gataaaacgg taaaacctat acttattaag taatattatg
     2881 taacaatcat tttgcatgaa tgcaatatgt gatggtctta tacacttttt atcgcattct
     2941 ggttaaaatc atccagccag cctgaacaac tggcacctgc tgtgtcaccg gagaacccga
     3001 tggcgcaaca tataaaatcc cacaattctg aagccgtccc ggccattaag cgggggcggc
     3061 attcgcacgt atttaaaacc cactggtacc agcatcctcc atgcactgaa gaacaggccg
     3121 aatggctgat tcagtgttac cgcaggcgcg gatacgaggt taagaaagcc ctcagcctca
     3181 attatcgtca ctggataatc tctgtcaggc tcccttattc cgaacgtcca ccgcgtccgt
     3241 cccgcacatt ccagcaacgt atctggaggt aacgtgcggg tattacttcg acctgttctg
     3301 gtgccggaac ttggcctggt ggtccttaag cccggacgcg aatcactgcc tgtttttcat
     3361 tgcggcaggg tgctggtgga gccggaaccg aaaagcatgc gtgctctgcc gtccgggttt
     3421 gtacctgccg ttcgccagcc gctggcggaa gataaatcat tactgccatt tttcagcgat
     3481 gagcgggtga tccgtgctgc gggtggcgct ggtgtactgt ctgactggtt attacgtcac
     3541 gtgaaatcct gccagtggcc acacggcgat tatcatcaca gcgaaaccgt cattcaccgt
     3601 tacagtaccg gcgcgatggt gttgtgctgg cactgcgaca accacctgcg ggagcagaca
     3661 tctgattcac tggagctact tgctcaacag aatctggccg cctggatgat tgacgtcatc
     3721 cgtcacgtaa tgaatggcgc acaggagcgt gaattatcgc tggctgaatt atcctggtgg
     3781 gcggtctgca atcaggtggc tgatgcgctt ccggaatctg tattgcgtcg ttcgctggga
     3841 ttaccggtgg aaaaaattcg ctccgtatac agtgagagcg acatcatacc gggagagcag
     3901 accgccacca gcatcctgaa gcagcggaca aaaaacatag cgccaccgct tcacgcccac
     3961 cagccacaaa ccacgccaca ggaaaagacg gtagtaagca tcgccgttga tccggagtcc
     4021 ccggaatctt tcatgaaacg gcctaaacgt cgtcgctggg ttaatgaaaa gttcacgcgc
     4081 tgggttaaga cgcagccgtg tgcatgctgt ggaaagccag ctgatgatcc gcatcacttg
     4141 attggccatg ggcagggcgg tatggggaca aaatcccacg atattttcac actaccgttg
     4201 tgccgggagc accacaacga acttcatgct gacccgctgg agtttgagaa aaagtacggg
     4261 tcccaggttg agttaatttt tcattttctt gatcacgcct ttgcgactgg cgtgctcggg
     4321 taaaagaggt gactgatgct catagatttg gttttacctt acccgccaac ggtgaacacc
     4381 tactggcgtc gtcgtggcag cacatatttt atctcggagg agggaaagcg ctatcgccgt
     4441 gctgtggcgc ttattgttaa ccagcagcgg ctgaaattaa gcctgtccgg aaggctggta
     4501 atcaggatta ttgcagagcc accggataag cgccgccgtg acctggacaa tattctgaaa
     4561 gcgccgctgg atgcgcttac gcatgcgggg ttgttaatgg acgatgagca gtttgatgaa
     4621 atcaatattg tgcgtggcct gcctgttcct ggcgggcggc tggggataaa aatcacagaa
     4681 ctggagtgcg catgaataac cagtatttac agtttgttcg tgagcaactc atgattgcca
     4741 ctgcggatct cagtggggcg acaaaaggcc agctggaagc ctggcaggag aatgccctgt
     4801 tcgatacagg gcgttacaga cgcaaaaaaa ttcgttatcg cgatgaggta accggaaaaa
     4861 tgatcacgcg ggataatcaa ccgatcccgg gtaaacaatc actggcgaaa ggctcatcaa
     4921 ttgcgctggt cagtccggtt gagtttgcaa catcatcgtg gcggcgtgcc gttctggaac
     4981 tggaagaaca tcagaaggcg tggttactgt ggtgctacag tggaaatgtt tgctgggaag
     5041 atcaggtcac tataacacaa tgggtatggg gtgagtttaa agctcaggct ggtaccagaa
     5101 aaattgcagg gaaaacgctg gatcgtctga agaaactgat ttggctggcg gcacaggatg
     5161 tgaagagtga gctggcaggg cgtgagacct atgaacatca ggagttagcg gtgttcatag
     5221 gagttgctaa atctacatgg acaaaaacac acaggcctca ctggttggct atgcggggta
     5281 gtgcagagat gctggacagg gatgctctta tttcagtaat gcgggtacgt tcacggcaaa
     5341 agtcgattaa tttagaacca tatcttgcaa aatagaactg aagagcatat attccgtgta
     5401 aatctgatat tttgcagatt ttgttcgcga tggcaaagta agcaaacccc gccgccgagc
     5461 gggttttttg ttggcttaac aggttataat gtacagtgcg aaactgcctg ttcagcagaa
     5521 tcatagagtg ttgactgata ctgtcgctgt accctgatgg gacttgtgag acttccgaac
     5581 tggaagccag gagtagcaat aaggtgatga caagcctgct gccctgtgta aaaggcacct
     5641 taatggtgcc tttgtcgttt ttatcaaaag atccccgcca ctggcgggtt ttttatgccc
     5701 gaaaaacggc acaggacgtt aaacgcgctg gtggtcgtga atagcgacct tttatcttgc
     5761 tggctttttc gacaagagtt attggtgtgt cacgttaacc ggaaaaggga aaaagacatg
     5821 ctgaaacagc aggatatgac agaaaccgcc agagtggtgt ttaatgaatt aagcgtcacc
     5881 gaaccggcga cagtcgggga gatagcgcag aatacttacc tttcacgcga acgctgccag
     5941 ttaatactga cccagctggt tatggcgggt ctggcagact atcagttcgg ttgttacaga
     6001 cgccttccgc agtgaaggct tttttatttg tggtaaatgg gcggctggtg ggtgttaggg
     6061 gcacccccca gccatctgct catgcgttgg gttcacaagc aaacctcagg cccactgctt
     6121 tgcgcaaaag cagaatgagc ctatcagaga caggcttaat gatccatgct taatactgta
     6181 aaaatatcca gttgtgagtt aatcaacgcc gactgccttg aatttatccg gtcgttaccc
     6241 gaaaattctg ttgacctgat agtcacggac ccgccgtact ttaaagtgaa gcctgagggc
     6301 tgggataacc agtggaaggg cgacgatgat tacctgaagt ggctggacca gtgtcttgcg
     6361 cagttctggc gggtgctgaa acctgccgga agtctttacc tgttctgtgg ccatcgcctg
     6421 gcatctgata tcgaaatcat gatgcgtgaa cacttcagtg tgctgaacca tattatctgg
     6481 gcaaagccgt ccggacgctg gaacgggtgc aacaaggaaa gcctgagggc gtatttcccc
     6541 gccacagagc gcattctgtt cgcggaacat tatcaggggc cgtatcgccc gaaagatgcc
     6601 gggtatgagg cgaagggcag ggcactgaaa cagcatgtga tggccccgct gatttcttac
     6661 tttcgtgatg cgcgtgctgc cctggggata acggcaaaac agatagtgga tgccacagga
     6721 aagaaaaata tggtgtcgca ctggttcagt gccagccagt ggcagttacc ggacgaaagc
     6781 gattatctga aattacaggc gctgtttgcc cgggtggcag aagagaagca tcagcgcgga
     6841 gaactggaaa agccacatca ccaactggtc agcacataca gtgaactgaa ccggcagtat
     6901 acggaactgc agagtgaata taagcatctg cggcggtatt tcggtgtgac ggtgcgggtg
     6961 ccgtacaccg atgtgtggac gcataaaccg gtgcagtact atcccgggaa acatccgtgc
     7021 gaaaaaccgg cagaaatgct gcagcagata atcagtgcga gcagtcgtcc gggtgacctg
     7081 gttgcagatt tcttcatggg gtcgggttcg acagtcaaag cagcgatggt gttggggcgt
     7141 cgtgctacag gcgttgagct ggagactgaa cgttttgagc agacggtcag ggaagttcag
     7201 gatttagcca gccagaacgg atgatattgc aggattagtt acgtaccgtt attatcctgc
     7261 gcccggccct ttagctcagt ggtgagagcg agcgactcat aatcgccagg tcgctggttc
     7321 aaatccagca agggccacca tatcacatac cgccattagc tcatcgggat agagcgccag
     7381 ccttcggagc tggctgcgcg gggttcgagt cctcgatggc ggtccattat ctgcatcatg
     7441 cgttgttagc tctgatgagt gaatcattgt cttttgagca acaggctatc atataagata
     7501 gtctcagatt tccttgttta ttctcgccgg atgcttcgta gatatttgca ggcatctgta
     7561 tgcaggggta ttattgcggc atatatcatc cggaaaaata aaaactacat gctgatccgg
     7621 agatgatagt tacaagtcct cccggatccc gcatatttac tatattttat agataattgt
     7681 tcttgttaat atggagatgg ataaatgtat tgccgtgctg taccggttct tgttttgata
     7741 ttagccagtc tgacaactgg ctgcacaaaa gatgtatcga caaataatta tgatgcttcg
     7801 ctgcatcact cagataaatt aataaagtcg caaaatttga gcttatcgga acgtacccct
     7861 gatgccagtg aagaaatccg gcagtatgcc attcaggtca gggaagccat agaagaacag
     7921 ttaaaggatg cgggtaagta ttcaggaaaa gagtgttcac tgagaatgta tatggccccg
     7981 aatggtcttc tgctacaggt taaaagagaa agcggtgatc ccgatttatg tcgtgaagcg
     8041 atgaatgcag taaagaatgc tgatatacca tcaccaccgt cttcaggagt atataaagca
     8101 tttagaaatg gcgtgctgga ttttaaactc tgactggggg agaagatttt ctcctgtcca
     8161 gatgattttg gggtgctgga aagatattgt gctgtgaagt gttaaattcc tcacaattca
     8221 gtaagttgac agttgcctgt cagactgggc atttgttaaa aaaatttcgc atggtgaatc
     8281 cccctgtgcg gaggggcgac tggtgaacgg tatgatctct ttgatgatcg taagcgagaa
     8341 tacgcgggtt tagtgtcacc gggctgaact caccgggagg cacccggcac catgctcatg
     8401 gtgatacaga aatgcggctt cagcccctct ctggaggggc tttcttatgg acaaaaaagc
     8461 ccgcgctggg agacgcgggc ggcaaggaat aaacaataaa acgtgaagta atatttcagc
     8521 tggcgaataa taccccatag taatcactct gcgcaactgc gcggcctttt tcgaattgcg
     8581 ggctgtagtc tcccttctgc cgggtggcct ggttgcagat ttcttcatgg ggtagggttc
     8641 ggcagttaaa gcagcgatgg cgctggggcg tcgtgctata ggcgttgagc tggataccgg
     8701 gcgttttgaa cagactgcgc aggcaatcag ggatatattt atcggtagct gtttgcagaa
     8761 gtaatctccc aaaattccat ttttggaatg cagtggttgt tgctgtagtg tgggcaagtt
     8821 ctggaactgg cttcgtccat tgcagatgat tgttagctgg atatcagtta cctcatggaa
     8881 atgttaaatg actcacaatt cggttgatgg actattgtct gattgatgga agtcttgtaa
     8941 aaacgagcca gcatagtgaa tccccctgtg cggaggggcg tctgacatgg tctccatagc
     9001 gatggtcaca atacgcagat tcagtagtca ggctgaatct accgggaggc acccggcact
     9061 atgctgctta tggtgtatac acagcgatcg gcccctctcc tgaggggctt tttttgtgcg
     9121 ggaaaaggct gtgttgttaa atgttaaatc cctcacagtt cagatggttg atgcttgcct
     9181 gtctgacggg gagttgttaa aaaagttctg catggtgaat ccccctgagc ggaggggcat
     9241 atcagcgcag gtgtttctac tctatccttt ctgtgcgggt tcaggtgctg atactgaact
     9301 caccgggagg cacccggcac catgtagtat acagagatta ggcatacatc caggctcctc
     9361 attgcaggag cctttttaca tgcaaaaaaa ccgctccagg gaagagcggt tggcaaggaa
     9421 taaataacaa aacgtgaagt aatttattca gcgccctaat aataccttac agtaatcaca
     9481 ttgcgcaact gtatgtaagg gcgtatttct tttttgcggg ctgttttgtg tgacttttgt
     9541 gtttccggag gtcagcccga gccttctctg actcagaaca ttattccggc cgggaggatt
     9601 catggcattt aaacactacg atgtggtcag ggcggcatcg ccgtcagacc ttgctgaacg
     9661 actgacacaa aaactgaagg aggggtggca gccatttggc agccctgtcg ccatcacgcc
     9721 ttataccctg atgcaggcta ttgcggcgga aggtgatgtc accacgccgg tgctggttaa
     9781 accatcgggt gacggggaga cggctatcag caagaccggt gagcctgaat attattatgt
     9841 tgtcgcgctg gcgggtcagt caaatggtat gtcttttgga gagggagtac cgttacctga
     9901 ttcttatgat cgccctgatc cgagaattaa gcagcttgcc cgtcgacgta ccgttacgcc
     9961 gcgaggcgtt ccctgcacct ataacgatgt tattccggca gatcattgtc ttcatgatgt
    10021 gatggatatg agtgaaaaaa atcacccccg ggcagacctc aataaaggtc agtatggttg
    10081 tgtgggtcag ggactgcata ttgccaaaaa actgttaccg tttataccgg cgaatgcggg
    10141 cattcttctg gttccatgct gtcgtggagg ctcgggagtg actacaggta atgagggaac
    10201 atttaacgcc tcttccgggg caagccctga ttctgcattg tggggagctg ataagccgct
    10261 gtaccaggat ctgttaagtc gcacgaaagt cgcgctggct aaaaatcccg gaaatatcct
    10321 gctggcggta atctggatgc agggggagag tgatctggct tcaggaagcc agagtcataa
    10381 tgctcttttt actggcatgg tacagaaatt ccgtgctgac atcgctgata tgtcagggca
    10441 gtgcgtgggg ggctcggcac tgagcgttcc gtgggtttgt ggcgatacga cgcattactg
    10501 gaaagaaaaa tatgctgcgt cttatgaaac tgtatacggt ggatataaaa ataaggcagc
    10561 gcagaatatt cactttgtcc ctttcatggt tgatggaagc ggggtaaatg tgcagaccaa
    10621 taatccggca gaagatcctg atattgaagg tattggctac tccggttcga agtggcgaga
    10681 cagttctgtc agctggacgt caggtgacag ggcaagccat ttcggttcat gggcgcggcg
    10741 tggaattatt tcagatcgtc ttgcaaccgc aattctgacc cacgcaggca aatacccggc
    10801 gtttatcacc gggcggtcag aaacggtagt tgcaggagat gaacgggaaa acgttacatc
    10861 cggaaatttc ccgtcacctg tggaggatgc tgcgttatca caggatgcac ctcagagaag
    10921 aacggttgtt gagtataatt cagctgttga gaaagacagc ctcggtggta actgggcagc
    10981 ccagcactgg aatgcccggg acggtgtcgc cgagtttgtt aatgatgaaa gcagacaggt
    11041 gctgaagatt aagcagcatg agggcaaaca gggaccgtgg atgctgtcag caggagtatc
    11101 agctgaagat gcccgtgctc tgtatgaaaa tggcggtgaa ataaaacttg aattcaggtt
    11161 gccggatggt acccaggttg cagatgacaa atacctcttt ggtgtttacc tgccagtttc
    11221 cgggtggact caggaaggca acggcagcaa tatgatggcc tcctttttca ctcagacaaa
    11281 agccagtaac ctggatgtca tgtatcacgg taaaacacag cctaatacaa aaattgagtc
    11341 attcggtgcg gttaataaca actggcacac gcttgtcttt aaatataaag gaggcggcaa
    11401 tcaccaggtt attccggtac ttgacggaga agagaaaaca ggttttgcac tgaaagaagg
    11461 tccggtcact tctgccttta cggtggataa catcacagtg acaggtatca gcgcaaatca
    11521 gggaaaatct tatcctgtca tgttccgcag tatcatggta agcattaacc aggcaacctc
    11581 ttcatcataa aacaaaaaaa cgccagcgca gaggagaacg ggaggataag tctgacgctg
    11641 gcggaggagt atcctcatgg agaacgatat gtaacacata tcgcctttct tttattgaaa
    11701 agtcatcgcc attgtcaacc ataacggtaa gaaactatga catttgttca tcaactgatg
    11761 ctgtacttct gtacggtggt atgcgtgctg tatcttcttt cgggtgggta cagagtcgtg
    11821 cgcgatttct ggcgcaggca gattgataaa agggccgctg agaaaatcag cgccagtcag
    11881 tcagccggaa gcaaacccga agatccgctc attccgtagt cactttcttg acaacacctt
    11941 tcaacgagaa aatcctatgt cagaaataaa atcgctggta actgctgagg cagtgaagga
    12001 agtcctgcgc tctgaagaag tcagaagcgc actgaaacaa aaactccgcc agaatcttga
    12061 ggcgcgtctt gatgcagaag tggatgccat tctggatgaa ctgctgggcg caccgactgt
    12121 tccggagccg gaaggcattg cggatgacag tgctgtttca gatggcgtcg ggtctcagcc
    12181 tgacggtagt gaagagcctc agcctgacgg cgaaatgatg atgtaaccat gcgcaggggt
    12241 gtcggtgtga gctgatgccc cacttgttgt tgtgagcttc cggattgcgg gagacggggt
    12301 atgtaccaga tggaaaaaat cacaacaggt ttgtcataca ccacgtcagc ggtgggaacg
    12361 ggctactggt tcctgcagtt gctggacagg gtttccccgt ctcagtgggc ggcaataggc
    12421 gtgctgggga gtctgctgtt tgggctgctg acatatctga cgaacctgta tttcaaaatt
    12481 aaagaagacc ggcgtaaggc ggcgcgggga gagtaagctg atgagcagga aactccgcta
    12541 tggtttatcg gctgccgttc tggcgctgat tggtgcaggg gcgtctgcgc ctgaaatctt
    12601 cgaccagttt ctggatgaaa aagaaggcaa ccacaccacg gcataccgtg atggcgcggg
    12661 tatctggacc atctgccgtg gagccacccg ggtggatggt aagcctgtta ttcctggcat
    12721 gaagttgacg aaggaaaaat gcgaccaggt taacgccatt gaacgtgata aggcgctggc
    12781 atgggtggag aaaaacatca aagtgccact gaccgaaccc cagaaagcgg gtattgcgtc
    12841 attctgcccg tataacattg gcccaggtaa gtgtttcccg tcgacgtttt ataaacgaat
    12901 taatgcaggc gatcgcaggg gagcgtgtga ggcgattcgc tggtggatta aggacggtgg
    12961 cagagactgc cgtattcgct caaataattg ctacggtcag atatcccgtc gtgaccagga
    13021 gagcgcgctg gcgtgctggg gaatcgacag ataagcagaa tattttgctg aaaaatgacg
    13081 ttgaccaacg cggacggata acacgaaatc ctgcgaactg gcaaaaccta gtgaataaaa
    13141 gtaaaaaccc cgtttgttgg cagcaagcgg ggttttgtgt ttcctgactc cggaaaagtc
    13201 aaaggagaaa gtgtgtttaa ttttagcaaa ctgattcggg agattcgagt gatggctgaa
    13261 aaattatcca cctggaagtt catccttatc tggctggtgt ttgtgattat ggcctccggt
    13321 tatttcatcg gtcagatacg ctggtggtga aatgaaccgc gttctgtgtg tggtcattat
    13381 tgccttgctg gtggcctgtg gtgcgcttag tctggggctg aatcattacc gtgataacgc
    13441 catcacctac aaagcgcagc gcgataaaaa agccagagag ctggagctgg caaacgcaac
    13501 cattactgac atgcagcagc gccagcgtga tgttgctgcg cttgatgcca gatactcaag
    13561 ggaattagcc gatgcgagag ctgaaaatga aactctgcgt gctgatgttg ccgctggtcg
    13621 taagcgcctg cggctcaacg ccacctgccc cggtaccgtg cgtgaagcca ccggcacctc
    13681 cggcgtggat aatgcaaccg gcccccgact ggcagacacc gctgaacggg attatttcaa
    13741 cctcagagaa cggttgatga caatgcagaa gcagctggaa ggggcgcagg aatatatacg
    13801 cactcagtgc actaagctgg ctttttatta tccggaggat acatgaagaa attacgggta
    13861 accgtagaac cttttcaggg aacaattccg ttccgtattt tgcagcgtgg tcgtgttctt
    13921 gttgaaggtt cgttcagtgg taaatgtacg caattacact cccggacctt tcaggtgaat
    13981 gccacgaatg aagagctaac cgttgagtgt acgatgaatg ccgctaaatg ccgcatggta
    14041 tccgctgcat tacagccagt gtgttgagcg accttattat ccatggcgcg gtattgtcgc
    14101 cgtattcccg tattaacaga gaccgcagcc cgacagggag actcctctgc gcgagtgtgc
    14161 ggggataatc aaaaacgata cacaccgggg tttaccgcgt taacggagcg cggcgttgtc
    14221 ccctcatggt cgctggtccg gtgcgatggt ggaagaaacc ggacgatgtg ttacctcgca
    14281 agctctgtta tgtcatgtgt ctgatttgtg atttaagtcg gataattgtc gttgccatta
    14341 agcagaggat tgatgaccga cagggtggca ttgttagaat aagacttatt cttatctgtg
    14401 ccgggaatga aaatgaaaag aaatcttccg ttaattattt tgttgtcttc tctggttatg
    14461 ggctgtacgc aacataaaac agatatgccc cgacagttgg ttaaggcatt accacaatat
    14521 ccggcctatg cagcggcaaa ttatataaag ggacgggttg atgtgaagtt tgatattggt
    14581 gctgatggta ctgtcacccg aattgagttt atccgttcag agccgcacca tctgtttgat
    14641 gagcaggttg taaaagcgat ggcaaaatgg cgatttgaga aggacaggcc gcgtaaaggc
    14701 gtgaagaaaa cgtttatctt tagtccttct gcaccctgat tatttcatca gaaattaatt
    14761 atcactctgt tgttattctg tacatcccgg cagggtaagt cttgttccgt cggatatgaa
    14821 gatgaaatat tgttggagga cagtgggtac ctgctcctgt aaccgaacgt tcatttctcg
    14881 ttatttgtca tgctggccgg gcgcagatgc gttgcatctg ttgccagcct tctcctgcag
    14941 gcttcaataa cccacgctga aaagttaccg gaccctttat gctcaagggc gatgttgatc
    15001 tgttcaatca tgtgattggg gaaacggata ttgcgggttg tggttctgcg ggtccggttt
    15061 ttcgatgaca tatttatttc ctttactgat tgccatatga cggggatttt acatggctca
    15121 gcttcgtaca ctccagagca gaatcaaaac actgaatacc cgacgggtga atattctgaa
    15181 gggtgaacag cgtcgtgtca gtggcagtgc acgtgtttcc ctcaagcgtc atatctggct
    15241 cagggacgcc gggcagtgct gtctctgtgg tcgtgtggtt gacctctgtg acagtgaact
    15301 cgatcaccga attgcacttc agttcggtgg tggtaatgag gagacgaatc tctggacgct
    15361 ctgtaccgaa tgccatcgac aaaagtctgt tcgtgaagcg gcgagtggta tgccggaccc
    15421 gacactgccg gaggtgtccg gaggtagtgg cagagcggac gacatcatcg gactgtaacc
    15481 cgacccgggg gggtatcatc cggcgtaaaa aactatcgct ccggacaccg cgccccctct
    15541 cacgcagaga aaaaattccc gtttcagggc agttaacatg ttaactggct gcccgggcat
    15601 ttttgcggtt tttatcttta ttattcagtt tgttgtgcgg aaaaaatgtt aactggcttt
    15661 ttcagcaaat gttaaccagg cagcagttaa catttgcggc atgagacgcc gggaaaaatg
    15721 ggctgaacca tacccggctg agtgcgttct ggacccggga ggaggctgtg ctgacaacgc
    15781 aaaaacgaaa atttgcgctg gcgctcatgt ccgggaaaaa caaaacagcg tcagccattg
    15841 ccgccggtta ttcggcgaag accgccaggg ttaaaggctc gcagctggca aaagatccgg
    15901 aggtgctcgc gtttattgcc cgtaagcagg gcgagacggt ggaggtggat gaggttcctg
    15961 tttaccggca gaaaaagacg cagacggagg agtgccatca ggaagcccct ccggcggaaa
    16021 agaacacccc ggtttcactg ccgcctgatg accggcaaag tctcacacca ctaccaggta
    16081 ttgattacat ggaagacggt cttcctgatc cggtaaaagc gatggggagg atcctggtgg
    16141 aaaacctgag cattgacccg aaactggcac tggatgcggc ctggcgtctg gcacagttca
    16201 cgcaccataa aaaaggggat gccggtaaaa aatcggcaaa aggtgacgcg gcgaaaaaag
    16261 cggctaaccg ttttgcggtg ccaccaccac cccgcctggt ggtgaataac gataatgagg
    16321 gcaacggatg ataccagggt ggagcacggc ctgcccggac tgggcagtac gcctgaaaaa
    16381 ggggctgtcg attattccgg ctccgattta cccggagcag gccgcacatg ccctggcgat
    16441 ttttaaacaa ctgcggattg tggatgcacc tggtagcccg acgttcgggg agtcctgtgc
    16501 acagtgggtg tttgacctgg tggcggccct gtttggctcc tacgatgcgc agaccggtgt
    16561 gcgccatatc aaggaagtgt tcattctgat acctaaaaaa aacagcaagt ccacgctggc
    16621 cgccgggatc atgatgacgg cgctgttact gaactggcgg caggcggcgg gctacaccat
    16681 tctggccccg accgtggagg tggcagccaa cgccttcaac cctgcccggg atatggtacg
    16741 acgcgacgat gatctggatg acctctgcca ggtacagacg catatccgga ccatcaccca
    16801 ccgggtgacg gacaccaccc tgaaggtggt ggcagccgat ccgaatacgg tgtccggtat
    16861 caagtccgtg ggtacgctga ttgatgagtt atggctgttt ggcaaacagt acaaggcgga
    16921 ggacatgtta cgtgaagcca taggcggcct tgcctcccgc ccggaagggt ttgtggtgta
    16981 tacgaccacc cagtcgaatg agccgcctgc cggggtgttc aggcagaaac tgcagtacgc
    17041 ccgggatgtg cgtgacggca aaattcatga tccgcacttt ctgccggtga tttttgagca
    17101 tccgccggag atggtggaga gcggtgagca cctgctgatg gaaaacctcg ccatggtcaa
    17161 tccgaatctc ggttattcgg tggatgaggc gtttctgtac cgggagtacc gtaaagcccg
    17221 ggaagccggt gaagagactt ttcgcggttt catgtcaaaa cacgccaatg tggaaattgg
    17281 tcttgccctg cgttctgacc gctgggcggg ggcggatttc tgggagcagc agggcaggcg
    17341 catcagtctg gacgatattc tgcagcgtgc tgatgtggtg acggtgggga ttgacggcgg
    17401 agggctggat gatctgctgg ggatgtatgt gattgggcgt gaccgggaga cccgcgaatg
    17461 gctgggctgg ggccatgcct gggcacatga aaccgcggtg gccagacgaa aaagtgaggc
    17521 agcccgtttt caggattttg ttgcctgtgg ggatatgacc attgtccgtc gggtcgggga
    17581 tgacacggcg gaagtggcgg agtatgtgcg tcgcattcat gaggctgagt tactggacca
    17641 tatcggtatt gacccgtcag gcgtggggca gattctggat tcactggcgg aagccgggat
    17701 ccccgatgag agtgtggtgg ggataagcca gggctggaag ctgggcggtg ccattaaaac
    17761 caccgagcga aaactggctg aaggggtact ggtgcatggt ggccagcccc tgatggcctg
    17821 gtgcgttggt aatgcccgtg tggagcccag aggtaatgcc attcttatca cgaaacaggc
    17881 cagcggacgg ggaaaaattg acccgctgat ggcgctcttc aatgcggtct ccctgatgtc
    17941 cctgaatccg gagccaaaaa agaaagaata tgcggttttt ttcatataac cctgttcacc
    18001 ctgtaaccat catgaaccgc tgcggcggtt tttttatttt caggaggctg atgtgactct
    18061 taaacgggcc tgttccctgc tgacggtgaa atccttcagt gaggatgagc gggtgatcac
    18121 cgggattgcg tcaacgcctt ctccggatcg ggatggggac atcctggagc cggagggcgc
    18181 ggagtttggc agtgcgatcc cgtttctctg gcagcatgac cattcccgcc cggtggggca
    18241 gtgtacggtg cgccgggtca gcgaagggct ggaaatcacg gcaacactgg cgaagcccgt
    18301 gccggatatg ccatcgcaac tggctgcccg gctggatgag gcctgggcgg ccattaagac
    18361 cgggctggtc agggggctgt ccgtgggctt ccgtcc
//
LOCUS       DAZFQD010000105        18393 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.105, whole genome shotgun sequence.
ACCESSION   DAZFQD010000105 DAZFQD010000000
VERSION     DAZFQD010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18393
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.105"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            13..438
                     /gene="nudI"
                     /locus_tag="ACHKTW_003606"
     CDS             13..438
                     /gene="nudI"
                     /locus_tag="ACHKTW_003606"
                     /EC_number="3.6.1.12"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HFO6464548.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVESGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL"
     gene            complement(477..1079)
                     /gene="ais"
                     /locus_tag="ACHKTW_003607"
     CDS             complement(477..1079)
                     /gene="ais"
                     /locus_tag="ACHKTW_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II)-phosphate
                     phosphatase Ais"
                     /protein_id="HFO6464549.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADIPDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQSKAPDKNIVIFTHNH
                     CLTYIAKNKRDATFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            1387..2526
                     /gene="arnB"
                     /locus_tag="ACHKTW_003608"
     CDS             1387..2526
                     /gene="arnB"
                     /locus_tag="ACHKTW_003608"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HFO6464550.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLAKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            2530..3498
                     /gene="arnC"
                     /locus_tag="ACHKTW_003609"
     CDS             2530..3498
                     /gene="arnC"
                     /locus_tag="ACHKTW_003609"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HFO6464551.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILLNRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHSEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            3498..5480
                     /gene="arnA"
                     /locus_tag="ACHKTW_003610"
     CDS             3498..5480
                     /gene="arnA"
                     /locus_tag="ACHKTW_003610"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HFO6464552.1"
                     /translation="MKTVVFAYHDMGCLGIEALLAAGYEISAIFTHTDNPGEKAFYGS
                     VARLAAERGIPVYAPDDVNHPLWVERIAQLSPDVIFSFYYRHLICDEILQLAPAGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRIAIAPDDIAIT
                     LHHKLCHAARQLLEQTLPAIKHGNILEIAQRENEATCFGRRTPDDSFLEWHKPASVLH
                     NMVRAVADPWPGAFSYVGNQKFTVWSSRVHPHASKAQPGSVISVAPLLIACGDGALEI
                     VTGQAGDGITMQGSQLAQTLGLVQGSRLNSQPACTARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDVVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LIVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFRPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPLRHHFPPFAGFRVVESSSYYGKGYQDVEHRKPSIR
                     NAHRCLDWEPKIDMQETIDETLDFFLRTVDLTDKPS"
     gene            5477..6367
                     /gene="arnD"
                     /locus_tag="ACHKTW_003611"
     CDS             5477..6367
                     /gene="arnD"
                     /locus_tag="ACHKTW_003611"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HFO6464553.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQKVIE
                     AKEAFHLRYNSDCRGAMPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            6367..8019
                     /gene="arnT"
                     /locus_tag="ACHKTW_003612"
     CDS             6367..8019
                     /gene="arnT"
                     /locus_tag="ACHKTW_003612"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HFO6464554.1"
                     /translation="MKSVRYLIGLFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFASLAMLMAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIATFVVSPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWPFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIDRQERLVLIQYRPK"
     gene            8016..8351
                     /gene="arnE"
                     /locus_tag="ACHKTW_003613"
     CDS             8016..8351
                     /gene="arnE"
                     /locus_tag="ACHKTW_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HFO6464555.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMVLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            8351..8737
                     /gene="arnF"
                     /locus_tag="ACHKTW_003614"
     CDS             8351..8737
                     /gene="arnF"
                     /locus_tag="ACHKTW_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HFO6464556.1"
                     /translation="MGLIWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(8731..8997)
                     /gene="pmrD"
                     /locus_tag="ACHKTW_003615"
     CDS             complement(8731..8997)
                     /gene="pmrD"
                     /locus_tag="ACHKTW_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HFO6464557.1"
                     /translation="MEWLVKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKVGD
                     LLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQCTAAGKTQ"
     gene            complement(9107..10462)
                     /gene="menE"
                     /locus_tag="ACHKTW_003616"
     CDS             complement(9107..10462)
                     /gene="menE"
                     /locus_tag="ACHKTW_003616"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008756 - o-succinylbenzoate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HFO6464558.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELASGF
                     AAQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQRVEGAHAATWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAEGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGSPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVPVADKEFGH
                     RPVAVVEYDQQTVDLGEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     Q"
     gene            complement(10459..11421)
                     /gene="menC"
                     /locus_tag="ACHKTW_003617"
     CDS             complement(10459..11421)
                     /gene="menC"
                     /locus_tag="ACHKTW_003617"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HFO6464559.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQETWEEAQSVLLAWVNNWLAGDCELPQMPSVAFGVSCALAELADTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSTLPVVEVDALERLL"
     gene            complement(11421..12278)
                     /gene="menB"
                     /locus_tag="ACHKTW_003618"
     CDS             complement(11421..12278)
                     /gene="menB"
                     /locus_tag="ACHKTW_003618"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HFO6464560.1"
                     /translation="MIYPDEAMLYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNIGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(12293..13051)
                     /gene="menH"
                     /locus_tag="ACHKTW_003619"
     CDS             complement(12293..13051)
                     /gene="menH"
                     /locus_tag="ACHKTW_003619"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070205 -
                     2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HFO6464561.1"
                     /translation="MILHAQAKHGKPGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VDLPGHGGSAAISVDGFDDVTDLLRKTLVSYNILDFWLVGYSLGGRVAMMAACQGLAG
                     LCGVIVEGGHPGLQNAEQRAERQRSDRQWAQRFRTEPLTAVFADWYQQPVFASLNDDQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(13048..14718)
                     /gene="menD"
                     /locus_tag="ACHKTW_003620"
     CDS             complement(13048..14718)
                     /gene="menD"
                     /locus_tag="ACHKTW_003620"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HFO6464562.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     STFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTRDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRICDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPQSERERFYLMPQNVHFEHAAAMFELKY
                     HRPQNWQELETAFADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(14807..16102)
                     /gene="menF"
                     /locus_tag="ACHKTW_003621"
     CDS             complement(14807..16102)
                     /gene="menF"
                     /locus_tag="ACHKTW_003621"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HFO6464563.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIQAKEFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANNPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(16181..16486)
                     /gene="elaB"
                     /locus_tag="ACHKTW_003622"
     CDS             complement(16181..16486)
                     /gene="elaB"
                     /locus_tag="ACHKTW_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HFO6464564.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKARAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(16541..17002)
                     /gene="elaA"
                     /locus_tag="ACHKTW_003623"
     CDS             complement(16541..17002)
                     /gene="elaA"
                     /locus_tag="ACHKTW_003623"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO6464565.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLET
                     CTHHWPDKPVYLGAQAHLQNFYQSFGFIPVTEVYEEDGIPHIGMAREVIQA"
     gene            17067..17984
                     /gene="rbn"
                     /locus_tag="ACHKTW_003624"
     CDS             17067..17984
                     /gene="rbn"
                     /locus_tag="ACHKTW_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HFO6464566.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPLEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGMLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
     gene            18172..>18393
                     /locus_tag="ACHKTW_003625"
     CDS             18172..>18393
                     /locus_tag="ACHKTW_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697257.1"
                     /note="ElaD; specific cysteine protease which targets
                     ubiquitin and ubiquitin-like proteins covalently bound to
                     target proteins; SseL in Salmonella is required for
                     macrophage killing and virulence, and it is secreted by
                     the salmonella pathogenicity island 2 type III secretion
                     system; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deubiquitinase"
                     /protein_id="HFO6464567.1"
                     /translation="MMVTVVSNYCQLSQKQLSQTFAEKFTVTEELLQSLKKTALSGDE
                     ESIELLHNIALGYDKFGKEAEDILYHIVRN"
BASE COUNT         4369 a         4889 c         4642 g         4493 t
ORIGIN      
        1 aggaataccc ctatgcgaca acggactatt gtatgccctt tgattcaaaa tgatggtgct
       61 tatttgctgt gtaaaatggc cgacgatcgc ggcgttttcc ccggtcaatg ggcgctttcg
      121 ggtggcggcg tggagtctgg cgaacgaatt gaagaggcac tacgccgcga aattcgcgaa
      181 gaactgggag aacagctgct tttgacagaa atcacgccgt ggaccttcag cgatgatatt
      241 cgcaccaaga cgtatgcaga tggtcgcaag gaagagattt atatgattta cctgattttt
      301 gactgcgttt ctgccaaccg agaagtgaaa ataaacgaag agtttcagga ctacgcgtgg
      361 gtaaaacctg aagatctggt gcattatgat ttaaatgttg ccacccgaaa aacgttacgt
      421 ttgaaaggtc ttctttaaca atgatgacag ccatcactgg ctgtcagatt gaaaatttag
      481 tggttaacga attccccatc cagataaact ttgccttttt ccacatgcat gactaaacca
      541 tccagataat caggtttaaa tgtcgcatca cgcttatttt tagcaatata tgtcaggcaa
      601 tgattatggg tgaaaataac gatattttta tcaggcgctt tgctttgtaa gtccttaatt
      661 gcactataaa tctcattacc gcactgaaga agtcgtttat ctaccgtcag ctttttaccc
      721 gcggaaaacc aggtcgccga ctgaatggtc cggacggtat tactggaata aagatcgaaa
      781 tcagggatgt cagcgctaaa agcgttgccc agttcacggg catcctgggt acctttaacc
      841 gtaatacctg ttttatctga caagcattga ttggttgaac gatcgcaacg ttcagcatgg
      901 cgaaacaaaa cgacaaccgg gtgctgctgt gccagtctgg ccaatgtttt attgtcgata
      961 cgtggcaaac cattgctact ccaggcggca tgagtaccca gtccggcaat tgcagcgagc
     1021 gccagtaaaa tgatgaaata tttttttgac ttcaatgatg agcggcaaaa agctaacacg
     1081 taaactccac ctatagacaa gcgcaaccag acaattaccg taaaattgag cgacatttct
     1141 ggcgataatt agcagttggt gtaatattaa aaatcctacg atgtcggcaa aatgcctcaa
     1201 aattttgcca aatgcaaagc ctaaataaga aaaaatataa aaatttcaat atttacgtct
     1261 aatattagtt tcttaaggtt aagttaatat tctatcctta aaatttcgct ccaaatggca
     1321 aaatatacac aacactcttt atagcaaata taagtggaca ggtattcaat ggcggaagga
     1381 aaagcaatgt cagaattttt gcctttttcg cgaccagcaa tgggcgtgga ggaactcgct
     1441 gcagttaaag aggttctcga atccggttgg atcacaaccg gtccgaagaa tcaggcgctt
     1501 gagcaagcct tttgccagtt gacgggaaat cagcatgcca tcgcggtcag ttcagccacc
     1561 gccggaatgc atatcacgct aatggcgttg gaaattggca agggcgatga agtgattacg
     1621 ccttccctga cctgggtttc aaccctcaat atgatttcct tgttgggtgc aacgccggta
     1681 atggtggatg tcgaccgcga tacgctgatg gtcacgcctg aagcgatcga gtcagccata
     1741 acgccacgca ctaaagccat cattccggtg cattatgccg gtgcaccagc agatattgac
     1801 gccattcgcg ccattggcga acgttacggc atcgcagtta tcgaagatgc tgcccatgcc
     1861 gtcggtacgt attacaaagg gcgacatatt ggcgcaaaag gtaccgctat tttttcattt
     1921 catgccatta aaaatattac ctgtgcggaa ggtggcctga ttgtaactga taatgaaaac
     1981 cttgcccgcc agctacggat gctgaaattt cacggtctgg gtgtcgatgc ctatgacaga
     2041 caaacctggg gccgtgcacc gcaggctgaa gtcttaacac cgggctataa gtacaatctg
     2101 accgatatta acgccgcgat tgccctgaca cagttagcaa aattagagca cctcaacacc
     2161 cgtcggcgcg aaattgccca gcaatatcag caagcactgg cagctctccc ctttcagcca
     2221 ttaagccttc ccgcctggcc gcacgttcac gcctggcatc tgtttattat tcgtgtcgat
     2281 gaacaacgtt gtggtatcag tcgcgatgcg ttgatggaag cgttaaaaga aagaggcatt
     2341 ggtaccgggt tacatttccg cgccgctcac acacaaaaat attatcgcga gcgttttccc
     2401 acgctgtcgt taccgaatac cgaatggaat agcgaacgca tctgttcttt gccgctgttc
     2461 ccggatatga ctaccgccga tgccgaccgt gtcatcacag cccttcagca actcgcagga
     2521 caataagcca tgtttgaaat ccaccctgtt aagaaagtct cggtggttat tcccgtttat
     2581 aacgagcagg aaagcttacc ggaattaatc aggcgcacca ccacagcctg tgaatcgttg
     2641 gggaaagagt atgagatcct gctgattgat gacggcagta gcgataattc cgcgcatatg
     2701 ctggtcgaag cctcacaagc ggagaacagc catattgtgt ctattttgct taaccgcaat
     2761 tacgggcaac attcagcgat tatggcggga ttcagtcacg ttaccggcga cttaattatt
     2821 acccttgatg ccgatctcca gaatccgcca gaagaaatcc cccgcctggt ggcaaaagcc
     2881 gatgaaggtt acgacgtggt agggactgta cgccagaacc gccaggacag ctggtttcgt
     2941 aaaacagctt cgaagatgat taaccggctt attcagcgca ccaccggcaa agcgatgggt
     3001 gactacggtt gtatgctgcg cgcctatcgc cgtcatattg tcgatgcgat gttgcactgc
     3061 catgaacgta gcacctttat cccgattctg gcgaatatct tcgcccgccg tgccattgaa
     3121 attccagtac atcattccga gcgtgagttt ggtgaatcca aatacagttt tatgcgcctg
     3181 attaatttga tgtacgacct ggtgacctgc cttaccacaa cgccgctacg tatgctgagt
     3241 ctgctcggca gcattattgc gattggaggt tttagcattg cggtgctgct ggtgatttta
     3301 cgcctgacct tcggaccaca atgggcggca gaaggcgtct ttatgctatt tgccgtgctg
     3361 tttactttta ttggcgctca gtttatcggc atgggattac tcggtgaata tatcggcagg
     3421 atctacaccg atgtccgcgc ccgcccccgc tattttgttc agcaagttat ccgtccatcc
     3481 agcaaggaaa atgaataatg aaaaccgtcg tttttgccta ccacgatatg ggatgcctcg
     3541 gtattgaagc tctgctggct gccggttacg aaattagcgc catttttacc cataccgata
     3601 atcccggtga aaaagccttt tatggttcgg tggctcgtct ggcagcggaa agaggcattc
     3661 cggtttatgc gccggatgac gttaatcatc cgctgtgggt ggaacgcatt gcccaactgt
     3721 cgccagatgt gattttctct ttttattatc gccatcttat ttgcgacgaa attttgcagc
     3781 tcgctcccgc aggtgcattt aatctgcatg gttcgctgtt accaaaatat cgtggtcgcg
     3841 cgccgctgaa ctgggtgctg gtcaacggtg aaacggaaac tggcgttaca ttgcaccgaa
     3901 tggtgaaacg tgccgatgcc ggggccattg tggcgcaact gcgcattgcc attgcgccag
     3961 acgatatcgc tattacgctg catcataaat tgtgccatgc cgcgcgccag ctactggaac
     4021 agacattacc cgccattaaa cacggtaata ttctggaaat cgcccagcgc gaaaacgaag
     4081 ccacctgttt tggtcgcaga acgccggatg acagtttcct tgaatggcat aaaccggcat
     4141 ccgtactgca caacatggta cgtgccgttg ccgatccgtg gccgggtgcc ttcagctatg
     4201 ttggcaatca gaaattcacc gtctggtcgt cgcgtgttca tcctcatgcc agcaaagcac
     4261 agccggggag cgtgatttct gttgcgccac tgctgattgc ctgtggcgat ggcgcgctgg
     4321 aaatcgtcac cggacaggcg ggcgacggca ttactatgca gggctcgcaa ttagcgcaga
     4381 cgctgggcct ggtgcaaggt tcacgcttga atagccagcc tgcctgcacc gcccgacgcc
     4441 gtacccgggt actcatcctc ggggtgaatg gctttattgg caaccatctg acagaacgcc
     4501 tgctgcgcga agatcattat gaagtttacg gtctggatat tggcagtgat gcgataagcc
     4561 gttttctgaa tcatccgcat tttcactttg ttgaaggcga tatcagtatt cattccgaat
     4621 ggattgagta tcacgtcaaa aaatgtgatg tcgtcttgcc gctggtggcg atagccacgc
     4681 cgattgaata tacccgcaac ccgctgcgcg tatttgaact cgattttgaa gagaatctgc
     4741 gcattatccg ctactgcgtg aagtaccgta agcgaatcat cttcccgtca acttcagaag
     4801 tttatgggat gtgtagcgat aaatacttcg atgaggacca ttctaattta atcgtcggcc
     4861 cggtgaataa accacgctgg atttattcgg tatcaaaaca attacttgat cgggtgatct
     4921 gggcctatgg cgaaaaagag ggtttacagt tcaccctctt ccgcccgttt aactggatgg
     4981 gaccgcgact ggataacctt aatgcggcac gaatcggcag ctcccgcgct attacgcaac
     5041 tcattctcaa tctggtagaa ggttcaccga ttaagctgat tgatggcgga aaacaaaaac
     5101 gctgctttac tgatattcgc gatggtatcg aggcgttata ccgcattatc gaaaatgcgg
     5161 gaaatcgctg cgacggtgaa attatcaaca ttggcaatcc tgagaacgaa gcgagcattg
     5221 aggaactggg cgagatgctg ctggcgagct tcgaaaaaca tccgctgcgc catcatttcc
     5281 caccgtttgc gggctttcgc gttgtcgaaa gtagcagcta ctacggcaaa ggatatcagg
     5341 acgtagagca tcgtaaaccg agcatccgca atgcccaccg ctgcctggac tgggagccga
     5401 aaattgatat gcaggaaacc atcgacgaaa cgctggattt cttcctgcgc accgttgatc
     5461 ttacggataa accatcatga ccaaagtagg cttacgcatt gatgtcgata cctttcgtgg
     5521 cacccgtgaa ggcgtgccgc gtctgctgga aatcttgagt aagcataata ttcaggccag
     5581 catttttttc agcgtcggcc cggacaatat gggccgccat ctctggcgac tggtgaagcc
     5641 acagtttttg tggaagatgc tgcgctcaaa tgcggcatcg ctttatggct gggatatttt
     5701 actggcaggt acggcctggc caggtaaaga gattggtcat gccaatgccg atatcattcg
     5761 tgaagcggct aaacatcacg aagtcggcct gcacgcctgg gatcaccatg cctggcaagc
     5821 ccgtagcggt aactgggatc ggcaaacaat gatcgacgat attgcacgcg gtcttcgcac
     5881 tctggaagag attatcggtc aaccggtaac ctgttctgcc gctgcgggct ggcgtgccga
     5941 ccagaaggtg atcgaagcaa aagaagcgtt ccatttgcgc tacaacagcg attgtcgtgg
     6001 ggccatgccg ttccgtccat tgctcgaatc aggaaaccct ggcactgcgc aaattccggt
     6061 gaccttaccc acctgggatg aagtgattgg tcgggatgtg aaagcagaag attttaacgg
     6121 ttggttactc aaccgcatcc tgcgagataa aggcacgccg gtttatacca ttcatgcaga
     6181 agttgaaggc tgcgcttatc agcataattt tgtggatctc ctcaaacgcg cagctcagga
     6241 aggcgtgaca ttttgccctt taagcgaact gttatcagag acgttgccgc tcggacaagt
     6301 tgttcgcgga aatattgccg gacgtgaagg ctggctgggt tgccaacaaa ttgcgggtag
     6361 tcgctgatga aatcggtacg ttaccttatc ggcctcttcg cgtttattgc ctgctattac
     6421 ctgttaccga tcagcacgcg tctgctctgg caacccgatg aaacgcgtta tgcggaaatc
     6481 agtcgagaaa tgctggcatc cggcgactgg attgtgcccc atctgttagg gctacgttat
     6541 ttcgaaaaac ccattgccgg atactggatt aacagcattg ggcaatggct atttggcgcg
     6601 aataactttg gtgtgcgggc aggcgttatc tttgcgaccc tgttaactgc cgcgctggtg
     6661 acctggttta ctctgcgctt atggcgcgat aaacgtctgg ctctactcgc cacagtaatt
     6721 tatctctcat tgtttattgt ctatgccatc ggcacttatg ccgtgctcga tccgtttatt
     6781 gcattctggc tggtggcggg aatgtgcagc ttctggctgg caatgcaggc acagacgtgg
     6841 aaaggcaaaa gcgcaggatt tttactgctg ggaatcacct gcggcatggg ggtgatgacc
     6901 aaaggttttc tcgcccttgc cgtgccggta ttaagcgtgc tgccatgggt agcgacgcaa
     6961 aaacgctgga aagatctctt tatttacggc tggctggcgg ttatcagttg cgtactgacg
     7021 gttctccctt ggggactggc gatagcgcag cgggagcctg acttctggca ctattttttc
     7081 tgggttgagc atattcaacg ctttgcactg gatgatgccc aacatagagc tccgttctgg
     7141 tactacgtgc cggtcatcat tgccggtagc ctgccgtggc tgggattact ccccggtgca
     7201 ctgtacacag gctggaaaaa ccgcaagcat tccgcaaccg tctatttgtt gagctggacg
     7261 ataatgccgc tgctgttttt ctccgtcgct aaaggtaaat tgcccaccta tattctttcc
     7321 tgctttgcat ctctggcaat gctgatggcg cattacgctt tgctggcagc aaaaaataat
     7381 cctctggcgc tgcggattaa tggctggatt aacatcgctt ttggcgtcac tggcattatt
     7441 gccacatttg tggtctcccc gtggggacca atgaacacgc cggtgtggca aaccttcgag
     7501 agctataaag tcttttgtgc ctggtcgatt ttttcgctat gggcattttt cggctggtac
     7561 accttaacaa acgtcgaaaa gacctggcct tttgccgcgc tttgcccgct ggggctggcg
     7621 ttgctggtag gattttcaat tcctgacaga gttatggaag gaaaacatcc gcaatttttt
     7681 gtcgagatga cacaagaatc acttcagcca agccgctata ttcttactga tagcgtcggt
     7741 gttgccgcag gtctggcatg gagcctgcaa cgcgatgaca tcatcatgta tcgccagaca
     7801 ggtgagttga aatacggcct taattatccg gatgcgaaag ggagatttgt cagcggtgat
     7861 gagttcgcaa actggcttaa tcaacatcgt caggagggga ttattactct cgtgctttcg
     7921 gttgaccgcg atgaagatat caacagtctc gccattccgc ccgcagatgc catcgatcgt
     7981 caggagcgtc tggtgctgat tcagtatcgt cccaaatgat ctggctaaca ttagtctttg
     8041 ccagcttgct tagcgttgcc gggcagttgt gtcagaaaca ggcaacctgc tttgtggcga
     8101 taaacaaacg gcgcaaacat atcgtgctgt ggctgggact ggcgctggct tgtcttggtc
     8161 ttgccatggt gctctggctg ctggtcttgc agaacgtacc ggtaggcatt gcctacccga
     8221 tgttaagtct gaattttgtc tgggtgacgc tggctgcagt aaaactgtgg cacgaaccgg
     8281 tatcgccgcg tcactggtgt ggggtggcgt tcattattgg tggcattgtg atcctcggga
     8341 gtacggtgta atgggcctga tatggggatt attcagcgtc attattgcct cagttgcgca
     8401 attaagcctg ggttttgcgg caagtcatct gccgccgatg acgcacttat gggattttat
     8461 cgcggctctg ttagcttttg gcctggatgc ccggatactg ttactcgggc tgctgggtta
     8521 tctgctttcc gttttttgtt ggtataaaac gctacataaa cttgcgctaa gcaaagccta
     8581 tgccttgctc agtatgagtt atgtgctggt atggattgct tcaatggtac ttcccggctg
     8641 ggaagggact ttttcgctga aagcactact gggagtagcc tgtattatga gcgggttgat
     8701 gctgattttc ctgcccacga caaaacaacg ttactgagtt ttccctgccg ctgtacactg
     8761 ccgttcccac tcatcggggc tataactgct tgccgagagg actttcaccg tatgcagctt
     8821 ttcacgatta atacaataaa gcgcattctg caaaggcgag agtaaatccc ccactttcac
     8881 ggcgaagtca ctcttcactt cggcgatcat ttttatcgcg ccgccagcat cgcagagcat
     8941 aagtacatgt ctgttatctt gtttattgca gcacgatttt ttgaccagcc attccattgc
     9001 attatcctgt ttgctaagag ttttcacatc aatttacaga ttaacagata aactctttat
     9061 caacggcaat ggaatggcag aaatgagaag tgaaacggag ggttaattat tgctgacgtt
     9121 gcacccactc ttttagcgcc tgacgtgaaa ttttaatacc gccgtttttc agctccggcg
     9181 gtaaagtcag ccagcgcacc ggttgttgaa aacgagccag cttatctttc acccattcgc
     9241 caagatcaac ggtttgctgg tcatactcca ccaccgctac cggccgatga ccaaactcct
     9301 tgtcggcaac gggaacgata aacacctgca aaaccgcagg atgtgcagca ataacacgct
     9361 cgacttcttc cggctgaata ccctcaccgc cactgaagaa cagattgtct aaacgtccga
     9421 caatggtcag cttgccatta tgcatctcac cgcgatcgcg cgtagcgtac cagccttcgt
     9481 cattaaccag tgaaaccagt tgcccgttac gccagtaacc ttctgccata ctggcagccc
     9541 gcagccacac ttcattatta acgattttca cttcccgacc cggcagcggc gaaccaacgt
     9601 ctgccaggcc gtcggcttct ttcgcacaca ccgtggaggc aaactcggtc agaccatagc
     9661 cgcaaaagca acgaatcccc tgctcgcgcg cctgttccgt caactcgacc gggatagccg
     9721 cgccgccaag taacaccgct ttcagggaaa cggaactgcg gttaaccagc aaacgccaga
     9781 gttgtgttgg caccagtgaa gcgtgagtac agcctgccag catttgctcc aatggctgtt
     9841 tatcacgcac cgtcatccgc gcaccagcgt ataaccagcg ccacataatt ccctgaccgg
     9901 agacgtgaaa cagcggtaaa gagagcaacc agtcatcgtg atcgccaaac ggaatcaacg
     9961 ataacacccc ttcggcactg gcaagatggg cctgataagt atggacagcg gctttcggca
    10021 acccggtaga acccgaggtc aacgtcattg agcacagacg cgtcggctgc cacgtagcgg
    10081 catgtgcgcc ttcaacccgc tgaatgtgca gcgacgttaa cgccggaaac gtgttttccc
    10141 catccggcac cagcgcaaat tgcagcgtca gattgggcag caattcttca agcaacggtt
    10201 gcggcagctg agggttcacg ggcaacaccc gcgccccgca ttgcagtaac gccagccagg
    10261 cgagcagcgt ttgcggcgta ttccacgccc gcaacatcac gccgctgccc tcaacaacgc
    10321 cctgcgccgc aaagcctgag gctaattcat cgacgcgagc acaaagctcg cgccagttaa
    10381 gttgctcgtc attaagacgt aaggcgatgg cttctccccg cacttgccgc cagtgacgcc
    10441 acggccagtc agagaagatc ataacaaccg ctccagtgca tcaacttcca cgacaggcag
    10501 cgtgctaccc ggccagcgac gtacctgctg cgcctgcatc agatccagcg tgtccagccc
    10561 tggaatggtg tccggcgtta accaggcggc aatccgcgcc agttgcgtta agcctaagct
    10621 cgattcaatg gaagaactga tcaccgccgt cagccccagc gcgtgcgccg cctgtacctg
    10681 ctcgcgtact ttctccagac tgcccgtgag cgtgggtttg ataactaccg cgcgcacgcc
    10741 ctcttcagcc acaaaggcaa aatccggctc gcgcaggctt tcatcccagg caatggcaat
    10801 gccggtttca cgggcaaacg ctcgcgaatc atcgcgggtt ttgcacggct cttcgagaaa
    10861 cgcgatgcgg tggcgataat ccgggttaac atatttggca aactgctggc ctttcagcgg
    10921 tgtccaggcg cgatttgcgt caagacgcaa atgcagatcc ggaattgcct ccagcaacag
    10981 attaaccacc atgccgtcgc gcacggcttc gtacaatccc actttgacct tcgccacttt
    11041 ctcgcctggc atatcagcaa gtttgaggat cagatcgtca ggatcgccat tacacagtgg
    11101 tgccgcacgg taattggccg cctgcgggag cgtatctgcc agctccgcca atgcacagct
    11161 tacgccaaag gcaacggaag gcatctgcgg tagctcgcaa tcgcctgcca gccaattatt
    11221 tacccaggca agcagcacgc tttgcgcctc ttcccaggtt tcctggctga agcccggcag
    11281 tggggagatc tccccccacc cttcgcgctc gccctcacgc aggcaaacgt acagcccgtc
    11341 gcgggttttt aaccgcctgt cgcgcagaac cacccccgcg tccatgggga tctgccagcg
    11401 gtatacctgc gcgctacgca ttacggattc cgtttaaatt tgctgaaatc aggctgacgt
    11461 ttctggttga aagcgttgcg accttcctga ccttcttccg tcatgtagaa cagcatggtg
    11521 gcgttgcccg ccagctcctg cagccccgcc tgcccgtcac agtcggcgtt cagcgcagct
    11581 ttcaggcagc gcagcgccat cgggctgttt tgcagcattt cgcggcacca acggacggtt
    11641 tctttttcca gatccgccag cggtaccacg gtgttgacaa ggcccatatc cagcgcctgt
    11701 tttgcgtcgt actgacggca caggaaccag atttctcgcg cttttttctg cccgacgata
    11761 cgagccatgt aggaagcgcc ccagccgccg tcgaaggaac cgactttcgg gccagtctga
    11821 ccgaagatgg cattatctgc tgcgatagtc aggtcgcaca tcatgtgcag aacgtgacca
    11881 ccgccgatgg agtagccagc caccatcgcg acaaccggtt tcggacaggt acggatctga
    11941 cgctggaagt ccagcacgtt caagtgatgt acgccggaat catctttata gccgccgtaa
    12001 tcaccacgca ctttctggtc accaccggag cagaacgctt tatcgcctgc gccagtcaga
    12061 atgatcacgc cgatattgtc gtcataacgc gcatctgcca gtgcctggat catctctttt
    12121 accgtcagag gacggaaggc attgcgcacc tgcggacgat taatggtgat ttttgcgata
    12181 ccgtcggtgg atttttcata acgaatgtcc tcgaaacctt cggagcagtc gtgccattca
    12241 accggtgcgt aaagcattgc ttcatcagga taaatcatat tgtgtccttt agtcagaaac
    12301 gcaagatctg cgccagactt gcgataacgc cagcgggatt ttcccgatgc gcgttatgtc
    12361 cggcgcgagg aatgacatgg cagtcggcag ccagttccgc cgccagggcg cggaatttgc
    12421 tgtcacgttc accacataaa taataaaacg caaatgtgcg ggcgctaagg ttagcacgta
    12481 aatcaggctg gacggcgaga gaagtcgcct ccagcatggc ggcaagggtt gcgccattat
    12541 tgttgctgcg cagcgccacc agctcccggc gttgatcgtc attgagcgag gcaaaaacag
    12601 gctgttgata ccagtcggca aataccgccg ttaacggttc tgtgcgaaaa cgctgcgccc
    12661 attggcgatc ggaacgctga cgttccgcac gttgttcagc attttgcagc cccggatgcc
    12721 cgccttcgac aataacccca caaagccccg ccagcccctg gcaagccgcc atcatcgcca
    12781 cccgtccgcc aagcgagtac cccaccagcc aaaagtcaag gatgttgtaa ctaaccaagg
    12841 ttttacgcag taagtcggtg acatcatcaa atccatcgac gctaatcgcc gccgaaccac
    12901 catgacctgg gagatcaaca tacaaccgtg agtagtcggc aaacgcctcg cccacttctt
    12961 gccattcgtg gcaatcgccg gaaaaaccgt gcaaaaacac cagccagggt aaacctggtt
    13021 ttccgtgttt tgcctgcgcg tgcaggatca taaatggctt acctgcgcca gaagttgctg
    13081 gagcgtttgc gcaccatcgg tgtcgttaac caccatttca atcaccgtgg tggttggcgt
    13141 gcgccaggcg tcggcaaatg ccgtttcaag ttcctgccag ttttgcggac gatgatattt
    13201 cagctcgaac atcgcggcgg cgtgctcaaa atggacgttt tgcggcatca gatagaaacg
    13261 ctcacgctcg ctttgcggcg ttggcaacag cgagaaaatt tgcccgccgt tgttgttcac
    13321 cacaattaat accagcggcg cagaaacctg acgcaataac gccagcgcgt tgagatcgta
    13381 aagtgcggag agatcgccca caatcgccag cgtcggtttg ccgcttgccc gctgaacgcc
    13441 tgcggcggtc gaaagcagtc cgtcgatacc gctggcccct cggttgctgt acaccgggta
    13501 acctgccgga agttgcgaaa gcgcatcaat cagacgtacc accaggctgt taccaacaaa
    13561 caattgcccc tgttcaggca gatagtcgca gatgcgatgc gccagttgcg cttcgccaaa
    13621 cgcatcacgg cgggcaataa ccgcctgcat tgcctgttcc gccaggcgcg ggatttcaac
    13681 gcaccagggc tggcgttttt ctgccggatg cagctccagc cagtcggcaa tattggcaat
    13741 taagcgacgt ccgcgatggt gtgccggatc aagtcgccct tcaatgtcat caacaatcca
    13801 gtactcttct ggttcacagc ttgcctgcca ttgcaggagc cgtttgcccg tcaggctgct
    13861 tcccagttgc accacaattt gcgcctgctg cagctcgctg gtcgctttgg cattgcctaa
    13921 ccaaagatcg gcacacggca gcggctgccc ggtttgtgac agcacatcgc caatcagcgg
    13981 ccagccaaga gtttgcgccc acagggcaac ttttttgccc tcttccgcac tcatgcgccc
    14041 ggcaaccacc acgccgcgct tttgtcgcca gaagaaccag tcgcgctgtt tttcactttc
    14101 cagacgaggc gcttcacgca gccacggttt gtcgtcctgc caccagtcac ccagacgctg
    14161 ttgccagcta agcccggtat cgtccatttc gccatacagc ggttcagcaa acgggcagtt
    14221 gatatggact ccccccgcat gaagcgtacc gagagcgtgg tcgatggtag aaaccagcca
    14281 acgtgcgggg atatcccggg tcgggcgcgg caatgaaata ctgtgcgtgg ggtgagaggc
    14341 gaacattccc ggctggcgaa ttgcctgatt cgcgccgcag tcaattagct ccggcgggcg
    14401 atcggcggtt aacaggatca gtttttctcc ggttaaccca gcttcaatca gtgccggata
    14461 gagatttgct accgccgtgc cggaggtcac aatcaccgcc accggctgct tgctgacttt
    14521 cgccagcccc agcgccagat gtcccagtcc acgctcatcg aaatgggtgt ggtgaatgaa
    14581 tgtggaattc tccgccgccg ctaacgttaa cggtgtagaa cgcgagccag gggcgataca
    14641 gatgtgtctg acgccgtgac gcgttaatgc ttccagaatg accgccgccc agcgtcggtt
    14701 aaatgcgctt actgacatga gtttgtccgg tatcaatatt gcggctaagt ataaggagct
    14761 acaaaaatag attattgata tgaatcggta atgatgcgac tcattactat tccatctgta
    14821 ataaagtacg cagccctgcc gctttgttgt cgatttcctg ccactcttgc tcggggtcgg
    14881 aaccacggac aatgcccgcg ccagcatata atcgcacgac attgccgcta atttttgctg
    14941 agcgcaggga aacgcagaat tcgctttgtt gtaatgagag atagcccgca gaaccggcgt
    15001 accattctcg ggtgaacggt tcgtgacggg cgataaactg tcgcgccaga tcgcgcggta
    15061 agccagcaac tgctgccgtt ggctgcaact gatgtaaaca gatcacatca tccgctttgt
    15121 tgagtgaagt ccagatacag cggcgaagat gctgcacttt acgcagacgc agtacctgcg
    15181 gcggtaaaac atccagcgtc tgggtatcgg cctgtaatcg ttggcagata tcttccacca
    15241 ccagcatgtt ctcgcgctgg tttttatcat ccgccatcag ccactctcct aactgctgcg
    15301 cctgcttatc atcaggatta tttgctactg ttcccgccag cgcttcagta cgcagcgctt
    15361 tgtcacgccg ccgccataac cgttccggtg aagagccaag aaaagcattt tcgccatcaa
    15421 aggccatgta aaaatggtag caattcagat tcagtcgacg actggcagcc atcatcgccg
    15481 ccgcgttgac cggacttgcg aaatgcaggt cagttgcccg agcgagcacc actttgtcga
    15541 gctcaccttc ggcgatggtt ttcgttgcca gttcgattaa ttgcgtccag cccgttttgt
    15601 ccggccagtg ttgttctcgc gtggtggtta aatgtaaccc aggcaagggc ttgatactca
    15661 ccagtgtggc gataaattct tttgcctgaa tcgcatcgtg ctgaagggag ctttcgctga
    15721 atagcgtcag ccgcagcgtg gctttaccgc cacagcgtcg ccattccagg cggggtaaaa
    15781 gtaaattgcc ctgcgacggg tcaaaagcat tcagccccca aatgcgtaag tcggcgtgtt
    15841 ccgggtgctg gcgaagaaaa cgttgtgcct ggtccaacga cgtaaaacgg gtaatcgcgc
    15901 ccaggacggc agcttcttca tcaccattac gttgttgcca gtagaattgc gggtacgttt
    15961 gctgactggc cagccagctc aaggcatcaa aagcgtcttt gagagggaaa ggaatatcga
    16021 taacccgaat gccgggtgtc gccggaatct cttgcgacaa atggcgcagt agattttcca
    16081 gcgccgtagt aagtgattgc acgcggacct ctccctgtta aaagcctcac attatacggg
    16141 gtactacaaa aaaaatgcag taccccggtg tagggaggtt ttaacggcgt gccagcaaca
    16201 gtcctagtac cagcccaacg gccgcgccca caccaattcc ttgccagggt ttttcgtgga
    16261 cgtagtcatc agcacgataa actgcctgct tcgcccgata gtaataactg tctgaagcct
    16321 ggctaacccg ttttttcaca tcatccagcg ctttttctgc acgcgctttc agctcaacat
    16381 atttctgatc ggcgggatcg ccagaggagc ggagcacctc ttccagtgtt tcactaagca
    16441 gcgtcaggtc gtcatcgata cgtgtatcac caaactgatt agacattctc gttctccatt
    16501 tgcgtaaaac ctgtcactaa ctatagacaa gggttgttga ttacgcctga attacctcgc
    16561 gtgccatccc aatgtgtggg ataccatctt cttcatagac ctccgtcaca gggataaaac
    16621 cgaaactctg gtagaagttt tgcagatgcg cctgcgcccc gaggtaaaca ggcttatcag
    16681 gccagtgatg cgtacatgtt tcgagtgttt tactcatcag ttgctgcccc actttttcgc
    16741 cacgcaacgc ttcactgaca atcacccgac ctataacgac cggctcaaga tcatcatcac
    16801 ttttcagaat cctcgcatat gccaccagtt catcattttt ccaaccaaga atatggcgat
    16861 tatcccccgt caaatcatca ccatcgatat cctgataagg gcaattctgc tcgacaacaa
    16921 acaccgcgca acgcagttgt aataaggcat aaagctggga aaccgataat tcagagtgat
    16981 gcagatcttg ccattcaatc ataatgctct ccttttagat gtatgcctta tactagccgg
    17041 aatatttttt gaaacgtgat gaactcatgg aattaatttt tttaggtact tcggccggtg
    17101 tgccaacccg cacgcgcaat gtcacggcaa tattgcttaa cctgcaacat ccgacccaga
    17161 gcggactttg gttgtttgac tgcggtgaag gcacccagca tcagctactg cataccgcct
    17221 ttaaccctgg aaaactggac aagattttta tcagtcacct tcatggcgat catctttttg
    17281 gtttacccgg cttgctgtgc agtcgttcta tgtcaggcat tatccaaccc ttaacgattt
    17341 atggtccgca aggtatccgt gaatttgtgg aaaccgcgct gcggattagc ggctcatgga
    17401 ccgattatcc gctggaaatt gtcgaaattg gcgctggcga aattctcgat gatggcctgc
    17461 gcaaagtaac cgcttatccg ctggaacacc cactggaatg ttatggctat cgtattgaag
    17521 aacatgataa accgggtgca ttaaatgccc aggcattaaa agctgctggc gtgccgcctg
    17581 gtccactgtt tcaggaatta aaagcgggca aaacaatcac cctggaagat ggaaggcaga
    17641 ttaacggcgc agattactta gctgctccag tgccaggtaa agcgctcgct attttcggcg
    17701 ataccggccc ctgcgatgcc gcacttgatc tggctaaagg tgtcgatgtc atggtgcacg
    17761 aagcgacgct ggatataacc atggaagcca aagccaatag tcgcggccat agctctacac
    17821 gccaggctgc gacactggcc cgtgaggctg gagtcggtat gctaatcatt acccacgtca
    17881 gctcgcgcta tgatgacaaa ggttgtcagc acctgttacg tgaatgcagg tcaattttcc
    17941 cggcgactga actggcgaat gatttcaccg tgtttaacgt ttaactctgc ttattattaa
    18001 aaacagggcg aaacttgccc tgttatcgca acccgcgcta catatatcca cccacacggg
    18061 catattgagc gatttttgat atttattcat ctcctcacgc actatattca gagcaataag
    18121 ttgcctctat ttatcttgat tcagcacaac ctttgcaact aaaaggagcc catgatggtt
    18181 acagttgtca gcaattattg tcaattatct caaaagcaac tcagtcagac atttgcagaa
    18241 aaatttactg tgaccgagga attactgcag tctttaaaaa aaacagcgtt atccggagat
    18301 gaagaaagca ttgagttact gcataatatt gcgttaggtt atgataaatt tggaaaagaa
    18361 gctgaagaca ttctttacca tattgttaga aac
//
LOCUS       DAZFQD010000106        18347 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.106, whole genome shotgun sequence.
ACCESSION   DAZFQD010000106 DAZFQD010000000
VERSION     DAZFQD010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18347
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.106"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..587)
                     /gene="rsmG"
                     /locus_tag="ACHKTW_003626"
     CDS             complement(<1..587)
                     /gene="rsmG"
                     /locus_tag="ACHKTW_003626"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFO6464568.1"
                     /translation="MLNKLSSLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDG"
     gene            complement(651..2540)
                     /gene="mnmG"
                     /locus_tag="ACHKTW_003627"
     CDS             complement(651..2540)
                     /gene="mnmG"
                     /locus_tag="ACHKTW_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFO6464569.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     LYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASQHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGGIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            complement(2919..3362)
                     /gene="mioC"
                     /locus_tag="ACHKTW_003628"
     CDS             complement(2919..3362)
                     /gene="mioC"
                     /locus_tag="ACHKTW_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HFO6464570.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEAGFTTETLHGPLLEDL
                     SASGIWLIISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            complement(3452..3910)
                     /gene="asnC"
                     /locus_tag="ACHKTW_003629"
     CDS             complement(3452..3910)
                     /gene="asnC"
                     /locus_tag="ACHKTW_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HFO6464571.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            4062..5054
                     /gene="asnA"
                     /locus_tag="ACHKTW_003630"
     CDS             4062..5054
                     /gene="asnA"
                     /locus_tag="ACHKTW_003630"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HFO6464572.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EERLSPLHSVYVDQWDWERVMGDGERQFSTLKSTVEAIWAGIKATEAAVSEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRENVPSLL"
     gene            complement(5059..6510)
                     /gene="viaA"
                     /locus_tag="ACHKTW_003631"
     CDS             complement(5059..6510)
                     /gene="viaA"
                     /locus_tag="ACHKTW_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HFO6464573.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQLIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMEAFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVIERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(6504..8000)
                     /gene="ravA"
                     /locus_tag="ACHKTW_003632"
     CDS             complement(6504..8000)
                     /gene="ravA"
                     /locus_tag="ACHKTW_003632"
                     /EC_number="3.6.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HFO6464574.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKE
                     IGEITLPDHVFELIFMLRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTVIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERNALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSAQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEASLQDVGAQIRQAQQC"
     gene            8223..10091
                     /gene="kup"
                     /locus_tag="ACHKTW_003633"
     CDS             8223..10091
                     /gene="kup"
                     /locus_tag="ACHKTW_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HFO6464575.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAVHFFLEYKTVSFIAL
                     GAVVLSITGVEALYADMGHFGKFPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            10258..10677
                     /gene="rbsD"
                     /locus_tag="ACHKTW_003634"
     CDS             10258..10677
                     /gene="rbsD"
                     /locus_tag="ACHKTW_003634"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HFO6464576.1"
                     /translation="MKKGTVLNSDISSMISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            10685..12190
                     /gene="rbsA"
                     /locus_tag="ACHKTW_003635"
     CDS             10685..12190
                     /gene="rbsA"
                     /locus_tag="ACHKTW_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
                     /protein_id="HFO6464577.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVMALVGENGAGKS
                     TMMKVLTGIYARDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWKTMYAEADKLLAKLNLRFKSDKLVGDLSIGDQQMVEIAKVLSFES
                     KVIIMDEPTDALTDTETESLFRVIRELKSQGRGIVYISHRMKEIFEICDDVTVFRDGQ
                     FIAEREVASLTEDSLIEMMVGRKLEDQYPHLDKAPGDIRLKVDNLCGPGVNDVSFTLR
                     KGEILGVSGLMGAGRTELMKVLYGALPRTSGYVTLDGHEVVTRSPQDGLASGIVYISE
                     DRKRDGLVLGMSVKENMSLTALRYFSRAGGSLKHADEQQAVSDFIRLFNVKTPSMEQA
                     IGLLSGGNQQKVAIARGLMTRPKVLILDEPTRGVDVGAKKEIYQLINQFKADGLSIIL
                     VSSEMPEVLGMSDRIIVMHEGHLSGEFTREQATQEVLMAAAVGKLNRVNQE"
     gene            12195..13160
                     /gene="rbsC"
                     /locus_tag="ACHKTW_003636"
     CDS             12195..13160
                     /gene="rbsC"
                     /locus_tag="ACHKTW_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HFO6464578.1"
                     /translation="MTTQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALALGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGININK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            13185..14075
                     /gene="rbsB"
                     /locus_tag="ACHKTW_003637"
     CDS             13185..14075
                     /gene="rbsB"
                     /locus_tag="ACHKTW_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
                     /protein_id="HFO6464579.1"
                     /translation="MNMKKLATLVSAVALSATVSANAMAKDTIALVVSTLNNPFFVSL
                     KDGAQKEADKLGYNLVVLDSQNNPAKELANVQDLTVRGTKILLINPTDSDAVGNAVKM
                     ANQANIPVITLDRQATKGEVVSHIASDNVLGGKIAGDYIAKKAGEGAKVIELQGIAGT
                     SAARERGEGFQQAVAAHKFNVLASQPADFDRTKGLNVMQNLLTAHPDVQAVFAQNDEM
                     ALGALRALQTAGKSDVMVVGFDGTPDGEKAVNDGKLAATIAQLPDQIGAKGVETADKV
                     LKGEKVQAKYPVDLKLVVKQ"
     gene            14201..15130
                     /gene="rbsK"
                     /locus_tag="ACHKTW_003638"
     CDS             14201..15130
                     /gene="rbsK"
                     /locus_tag="ACHKTW_003638"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFO6464580.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPALVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            15134..16126
                     /gene="rbsR"
                     /locus_tag="ACHKTW_003639"
     CDS             15134..16126
                     /gene="rbsR"
                     /locus_tag="ACHKTW_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HFO6464581.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(16092..17519)
                     /gene="mdtD"
                     /locus_tag="ACHKTW_003640"
     CDS             complement(16092..17519)
                     /gene="mdtD"
                     /locus_tag="ACHKTW_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HFO6464582.1"
                     /translation="MSDKKNRSMAGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGGAMMMPVARLALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARHTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRVYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSKPNRVPSESE"
     gene            complement(17542..18234)
                     /gene="yieP"
                     /locus_tag="ACHKTW_003641"
     CDS             complement(17542..18234)
                     /gene="yieP"
                     /locus_tag="ACHKTW_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFO6464583.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWMTEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIVQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT         4407 a         4551 c         4797 g         4592 t
ORIGIN      
        1 tcgccatcca gggctggaac ctgaagttta accactgatt cgacctgata ttcttcgggc
       61 aacaaagcga tttcatcttc cggcatttgc cctttcagcg cgtagaaacg gccttgctca
      121 ccaggaagat ggtggcacca gctcaccata tcgttcagag aggcaaaagc gcggctaatt
      181 acgccatcaa atggcggctc tgaaggaaac tcttctaccc tgctctgtac tggttcaata
      241 ttctccagtt taagctcatg ttgcacctga cgaaggaaac gcacgcgttt accaaggcta
      301 tccaacagag tgaaatgggc ttcaggacgc acgatagaga gtggaatgcc tggcagtcct
      361 ggtccggtgc cgacatcgat aaaccgttca ccttgcagat acggtgccac cacaatgcta
      421 tcgagaatat ggcgtaccag catctcatta ggatcgcgga ccgaagtcag gttgtacgct
      481 ttgttccatt tatgcagcat attcacgtag gcaataagct ggtttttctg gtgatcggta
      541 agcgaaatac ctgcgtcttt cagcagtgag gagagtttgt tgagcacggt gattacctgt
      601 tcttgatgcg ttgcctggta agcgggtgct taccaggcat ttttaatgcg ttatgcgcta
      661 cgacgcagca taccctgttt tttcagccac accagcagaa tggagatggc cgcaggcgtg
      721 acgccagaaa tacgcgaagc ttggccgata gaggctggtt tgtgatcgtt aagtttggcg
      781 atcacttcgt tagaaagacc ggatacctgg cggtaatcca gtgtcgcggg tagcagggtg
      841 ttctcgttac gcagctgctt ttcgatctca tcttgctggc gcgcgatata accttcgtat
      901 ttaacctgaa tctcaacctg ttccgccgcc tgttcgtctg tcaacgcagg ggcaaacggc
      961 gtcagcgtgg ttaatttttc ataagtcatt tccggacgac gcagcagatc ttcaccactg
     1021 gcttcacggg aaagcggcgc agtcaggtga gcattcactt cggctgcagc ttccgccgac
     1081 ggggttaccc aggtcgattt cagacgctga cgctcacgct cgatattctc aagtttctcg
     1141 ttaaagcgcg cccaacgttc gtcatccacc aggcccagtt cacgaccgat ttcagtcaaa
     1201 cgcagatccg cattatcttc gcgtagcatc agacgatatt ctgcgcgcga agtaaacata
     1261 cgatacggtt ctttggttcc taaagtgcac aggtcatcaa ctagtacgcc gagatacgcc
     1321 tgagaacgtg ccggagccca accttctttg tcagcagaca gacgggcagc gttaagaccg
     1381 gccagcaaac cttgcgcagc ggcttcttcg taaccggtag tgccgttaat ctgaccagca
     1441 aagaacagcc cctggataaa cttgctctcc agcgtcggtt tcaggtcgcg aggatcgaag
     1501 aagtcatact caatggcata acccggacgc acgatcttcg cgttttccat cccctgcata
     1561 gagcggacga tttgcatctg cacatcgaac ggcaggctgg tggagatacc gttcggataa
     1621 atttcattag aggtcagtcc ttccggttca aggaagatct gatgctgatt tctgtcggcg
     1681 aagcgcataa ctttgtcttc gatcgacggg cagtagcgtg ggccgacacc ttcgatcccc
     1741 cctgcataca ttgggctacg atcgaggtta ctgcggatca catcatgggt tttctcgttg
     1801 gtatgagtga tataacacgg cacctgctgg ggatgctggg acgcattgcc cataaacgag
     1861 aataccggca ttgggttatc gccatgctgt tgcgccagca cactaaagtc gatagttcga
     1921 gcatcaatac gcggtggtgt cccggttttc agacgaccaa cgcgcagcgg cagttcacgc
     1981 aaacggcgag aaagcggaat ggacggcgga tcaccagcac ggccaccgct gtaattatcc
     2041 agaccgatat gaattttacc gtcgaggaac gtcccaacgg tgagcacgac ggctttggca
     2101 cggaacttca gtcccatttg ggtaacagca ccgaccacgc gatcgttttc gacaataaga
     2161 tcttcaaccg cctgctggaa gatcatcagg ttcggttggt tctccagcgc cgtacgtacc
     2221 gcctgacggt agagcacacg atccgcctga gctcgggtag cgcgaaccgc cggtcctttg
     2281 cttgcgttta gtatcctaaa ctggataccc gcttgatcga tcgctttcgc catcagaccg
     2341 ccgagtgcat ccacttcttt taccagatgt cccttcccaa taccgccgat cgccgggttg
     2401 cagctcatct gccccagagt gtcgatattg tgtgtcaaaa gcagagtctg ttgacccata
     2461 cgcgccgcgg ccatcgcggc ctcggtgcct gcatgacccc cgccaatgat gatgacgtca
     2521 aaaggatccg gataaaacat ggtgattgcc tcgcataacg cggtatgaaa atggattgaa
     2581 gcccgggccg tggattctac tcaactttgt cggcttgaga aagacctggg atcctgggta
     2641 ttaaaaagaa gatctattta tttagagatc tgttctattg tgatctctta ttaggatcgc
     2701 actgccctgt ggataacaag gatccggctt ttaagatcaa caacctggaa aggatcatta
     2761 actgtgaatg atcggtgatc ctggaccgta taagctggga tcagaatgag gggttataca
     2821 caactcaaaa actgaacaac cgttgttcct tggataacta ccggttgatc caagcttcct
     2881 gacagagtta tccacagtag atcgcacgat ctgtatactt atttgagtaa attaatccac
     2941 gatcccagcc attcttctgc cggatcttcc ggaatgtcgt gatcaagaat gttgatcttc
     3001 agtgtttcgc ctgtctgttt tgcaccggaa tttttgagtt ctgcctcgag tttatcgata
     3061 gccccacaaa aggtgtcata ttcacgactg ccaataccga ttgcgccaaa gcggaccgca
     3121 gaaagatcgg gcttctgttc ctgcaatgct tcatagaaag gagaaaggtt gtccggaata
     3181 tctccggcac cgtgggtgga gctgataatc agccagatcc ccgaggcaga taaatcttcc
     3241 aacagcggac cgtgcagcgt ttcggtggta aaacccgcct cttccagctt ttcagccagg
     3301 tgttctgcta catattcggc accgccgagg gtgctgccgc tgataagagt gatatctgcc
     3361 ataaaccgcc acctttatta agagtggcgt attgtacgct gtgaacgcgt tgggatctac
     3421 ctgtggaaaa gtatgggatt aaaaaagccg attatggctt gatggtacgc atgatcgggt
     3481 tttgcaggac gatcaatgtc tcggtggact gaatttcatc aattgtttgg atcttgttga
     3541 taagtacatg ctggagagcg tcgatcgaac ggcacatcac ttttataaag atgctgtagt
     3601 ggccggttgt gtaataggct tcagtgactt catcaaggct ttccagcttt gccagcgcgg
     3661 aagggtagtc tttggcgctc tttaatataa tgccgataaa gcagcctacg tcataaccga
     3721 gctgcttcgg gctgacatca atacgcgccc cggtaatgat ccccgcctgc ttcattttct
     3781 ctactcgaac gtgaatcgtc cccggactga cgccaaattg tttcgccagt tcggcgtaag
     3841 cggtgcgcgc attgcccatt aatgcttcca ggatgccacg gtccagattg tcgatcagat
     3901 aattttccat aggattttct tatgcagatt gatgattcat tctattttag ccttcttttt
     3961 taatgaatca aaagtgtgtt aggcttttta ttgaatgatt attgcatgtg tgtcggtttt
     4021 tgttgcttaa tcataagcaa caggacgcag gagtataaaa aatgaaaacc gcttacattg
     4081 ccaaacaacg ccaaattagc ttcgtgaaat ctcacttttc tcgccaactg gaagaacgtc
     4141 tggggctgat cgaagtccag gcgccgattc ttagccgtgt gggggatggc acgcaggata
     4201 acttgtcggg ctgtgaaaaa gcggtgcagg taaaagtgaa agctctgcct gatgcccagt
     4261 tcgaagtggt tcattcactg gcgaagtgga aacgtcagac cttagggcaa cacgacttca
     4321 gcgcgggcga agggctgtac acgcacatga aagcccttcg ccccgatgaa gagcgtcttt
     4381 ctccgttgca ctcggtctat gttgaccagt gggactggga acgcgtaatg ggcgacggtg
     4441 agcgtcaatt ctcgactctg aaaagcacgg tagaggcgat ctgggcggga attaaagcaa
     4501 ccgaagctgc agttagcgaa gagtttggtt tggcaccgtt cctgccggat cagatccact
     4561 tcgtacacag ccaggagtta ctgtctcgtt atccggatct cgatgccaaa gggcgtgagc
     4621 gggcgattgc gaaagatctt ggcgcggtat tcctcgtcgg gattggcggc aagctgagtg
     4681 atggtcatcg tcacgacgtg cgcgcaccgg attatgatga ctggagcacc ccgtcagagc
     4741 tgggccatgc gggtctgaac ggcgatattc tggtatggaa ccctgtactg gaagatgcgt
     4801 ttgagctttc ttccatgggg atccgcgtgg atgccgacac gttgaagcat cagttggcgc
     4861 tgaccggtga tgaagatcgt ctgcagctgg aatggcatca ggcgctgctg cgcggtgaaa
     4921 tgccgcagac catcggcggc ggtatcggcc agtctcgttt gaccatgctg ctgctgcaac
     4981 tgccgcatat cggccaggtt cagtgtggtg tatggccagc tgctgttcgc gagaacgtcc
     5041 cttctctgct gtaataattt atcgccgcca gcgtctgagc aggcggcttc gcatcccggt
     5101 atcaaagcgc cagatatgat cgaaaatgcg catgatgccg ggtttgccgt gtgccgacat
     5161 cgccacggca tgaaagcgat gctgatgtac ccgctgcagc tctttcactt tactcgtcac
     5221 gtcgtcaggc aaccgctgag cgataaaatc agaaatcacc accgcgtcgg catcaaacca
     5281 ttccctgctt tgcaagcgtt ccataatggc gcgaaaacaa ctggcaagat cggtgccgcc
     5341 acgaaactgc tggcttaaaa aacggattgc ttgttcgatg ccttgtgggc ctgaaagctc
     5401 ataacggacg atctcggtgg aaaatagcat aatatagcag cgccggtttt cagcgagagc
     5461 aatgcgcatc aaggccaggc agaacgcttt cgcacactgt tcattaaagc cgcccattga
     5521 gccggaagta tccacacaga caataaacgg cccgcgcggc tgttcatcgt aatctttatg
     5581 taccaccgga cgttcgatca ctttttcacg ccacgactca ccgtgcaggc gataggtgag
     5641 caactgtttt tccaccagcc gacggtaaaa ctcatactcc agttccgtta tccctagtgt
     5701 cgccagttct ggcggcagga gacgtaaaat atcatcgctt tgttgcagac catcaacctg
     5761 ctcaggaaca gttgccggtt cgcgcaccat ggtgcggaag gcttccatct gcgcatcgtt
     5821 acgcggtatt gatttggctt cccgggaacg ccccagctgt tctgccaggc gtttcagttc
     5881 cggctgttcg ttaagaaatt caccgtattt cacaatcaac tgatagtcgc cacgtttaag
     5941 ctgaccggcg ctcatatccc acagacgacc agctgcggta ttgttatctg cgagaatcgg
     6001 ttcaagttgt ccgctcagcg tcatgcgttc ctgaacttca cttaacagtt gttcgcgttc
     6061 ttcttctaat agctgttgat ttaacgtcgt tgcttgcacg atcagactta aacgccaacg
     6121 ctggaggaaa agcgtatgta acgctgaagt gatcgtgctg ttagcatcaa ccaactgtcg
     6181 ggcttgttct gcccatgggg aattcagacg atgcagtaag tccaggatct gtggtagctg
     6241 cacaataaac tgcggcgtgg agaggagctg gctttgctga tagcacatca cctcttcggt
     6301 gagttccggc gggactcggg catctttcag ccgactgcgc agcgcctcac gccagcgggg
     6361 aacatcatca gtgattgccg ccttcagtcg tgggaatttt tcaaagaaga ctgccagctg
     6421 cggtgaggcc agcagcgcga tgatcatctc ttcgatcaat ccctcttcgc tgacggccag
     6481 catcacatta agcgtatcca gcgttagcat tgttgtgcct ggcgaatctg tgcgccgaca
     6541 tcctgtaggc tggcttcaat gcgacctaac cagtcgccag ggataaacag acatttttgc
     6601 tgttcgctga ataaagcgtg ttgcttgcgc cagtcacttt caagctcttc cagttgttgt
     6661 tttatttcac ctggcagacc ttcagccgat gaaccgggaa gtgccagcgt actgccttgt
     6721 aaacttacat cgcgtacaac aagatgttgg gcgctatcca cttccagatt caatttctgg
     6781 gcaaagccga taccgttcag tttgccgcga atttcaccac ctttactcag ccactgttcc
     6841 agcgcgttac gctcaaagga gatatgaacc acttccatat catgcaattt taacggtttt
     6901 tgcagcagca gagtcagagt ggaagcagta acgttaacag gaagttgata ctgctgacga
     6961 cggctgaaaa tgccgcccag acgaattacc gttaaggctg ttttatcgct ttgttgctgc
     7021 tggagttgca ggtgacgttg cacaatcgcg ccaaggcgag tcaacatccc ttgctgttgc
     7081 caggcgtgac cggtcatcaa tacatcaatt tgttgttgta tcaaattcag gctttgcgcg
     7141 tcataccaca ggcaatcttt cagcaaaatg agatctaccg gggcaacagc actgcgaccg
     7201 ctaaaaaagg cgctggcctg caataatcgg atcgcttttt tccagcgacg atccgagaca
     7261 taaggcgcat ccggtaattt atccagttgc tggcgcagca taaaaatcag ctcaaataca
     7321 tgatcgggca gcgtaatttc accaatctct ttctgccagc gttcatattc ttcatctgtg
     7381 acctgcaagg catcaggaac cggattgtcg ttttcatcct gttgactggt cagcatggag
     7441 cggaaattcg ctttatcctg cactttatct aaccacagac gaatcagcat gcggtcatat
     7501 aacgcttcca gactgctgtc tgcttccggc agctcgttgg aggccgccac cagcagacgc
     7561 atcgggattt tttctacgtg tgcaccgttg cggaactggc gctcgttaat ggcggtgagc
     7621 aaggtattaa gaattgccgg gcccgctttc cagatctcat ccagaaagac aatttctgct
     7681 tccggcaggt aaccgctggt taaacgttca tagcgccctt catcttttag cgcctgaata
     7741 gaaaggggac caaaaacttc ttccggcgtg gagaagcggg tcatcagata ttcaaacgcg
     7801 cgggcattct gaaaggcgaa ttttaagcgc cgggcgatca aacttttggc aatacctggc
     7861 gggccaagga ggaacacact ttcaccactt aatgccgcta ataaacacaa gcggatggcg
     7921 tggctacgtt cataaagccc cttttccaac gaactgctca ggcgggaaat tctttccgct
     7981 aataaatgag ggtgagccat aatgaagtgg cgtcctttcg tcaaaagttc tgcgtaaatt
     8041 gcgagtatag acgtttcttg ctggtggcta aaatagtctc aaaggggggg tatttttctt
     8101 tgagccaggt taatgtggcc gcatttagga gtacgatttt gccgttaatc gtgcatactg
     8161 tgcgcttttt tgtgggccaa gggactaagc acacatttca tatttcaacg aaagactagt
     8221 ctatgagcac tgataataag caatcattgc ccgcgattac cctcgcggcg attggagttg
     8281 tctacggcga tattggtacc agcccgttat atacacttcg tgaatgtttg tccggccagt
     8341 ttggttttgg cgttgaacgc gatgccgtgt ttggcttttt atcgctgatc ttctggctgc
     8401 taatctttgt ggtttccatt aaatatctca ccttcgtgat gcgggcagat aacgccggtg
     8461 agggggggat cctgacgttg atgtcgcttg ccgggcgtaa tacgtcggcg cgaaccacat
     8521 caatgctggt gattatgggg ctaatcggcg gcagcttttt ctatggtgaa gtcgtcataa
     8581 cacccgctat ttcggtgatg tcagccattg aaggtctgga aatcgtcgcc ccgcagctgg
     8641 atacctggat agttcccctc tcaattatcg ttctcacttt attatttatg attcaaaaac
     8701 atggcacggc tatggtcggt aagctgtttg cgccgatcat gctgacctgg tttttgattc
     8761 tggcaggact ggggttacgt agcattattg ctaacccgga agtgctgcat gcgctgaatc
     8821 caatgtgggc ggtgcatttc ttccttgaat acaaaacggt ttcttttatt gcattagggg
     8881 cagtggtgtt gtcgattacg ggggtcgagg cgctgtatgc tgatatgggg cactttggta
     8941 agttccctat tcgcctggcg tggttcaccg tcgtattacc ttccttgacc cttaattact
     9001 tcggtcaggg agcgctgttg ttaaagaacc cggaagcgat taagaacccg ttcttcctgt
     9061 tggcaccgga ctgggcgctg atcccgctgc tgatcatcgc cgcactggcg acggtaatcg
     9121 cctcgcaggc tgtcatctct ggcgtcttct cattgacgcg tcaggcggta cgtctgggat
     9181 atttgtcgcc gatgcgcatt attcacacct ccgaaatgga gtcagggcaa atctatattc
     9241 cgtttgtgaa ctggatgctc tatgtcgcgg tcgtgattgt gattgtcagc tttgagcact
     9301 ccagcaacct ggcggcggcg tacgggattg cggtgaccgg aaccatggtg ctgacgtcta
     9361 ttctctcgac tactgtggca cgtcagaact ggcactggaa taagtatttt gttgcgctga
     9421 tcctgattgc tttcctttgt gtcgatattc cattgttcac cgctaacctc gataaactgc
     9481 tctccggcgg ctggttgcca ttgagcctcg gtactgtgat gtttatcgtg atgaccacct
     9541 ggaagagcga gcgtttccgc ttgctgcggc ggatgcatga acatggtaac tctctggaag
     9601 cgatgattgc ttcgctggag aaatcaccgc ctgttcgcgt gcccgggacc gcggtgtata
     9661 tgtcgcgtgc aatcaacgtc attccctttg cgctgatgca taaccttaaa cataacaagg
     9721 tattgcatga gcgggtgatt ctgttaactc tgcgcaccga agacgcgcca tatgtccata
     9781 acgtccgtcg ggtacagata gaacaactgt cgcccacttt ctggcgcgtg gtggcaagtt
     9841 atggttggcg agaaacgcca aacgtggaag aagttttcca ccgctgcggt ctggaaggat
     9901 taagttgccg gatgatggaa acctccttct ttatgtcgca tgagtcgttg atcctcggca
     9961 aacgcccgtg gtatttgcgt ctgcgcggca agctgtactt gctgctgcaa cgtaatgcgc
    10021 tgcgtgcacc agatcaattt gaaattccgc caaacagggt tatcgaactg ggtactcagg
    10081 tcgaaatcta acgccagacg cctcctttct ccataagggg gcgtttttgt tttcatggtt
    10141 aatcaccatg taaaacgttt cgaggttgat cacatttccg taacgtcacg atggttttcc
    10201 caactcagtc aggattaaac tgcgggtcag cgaaacgttt cgctgatgga gaaaaaaatg
    10261 aaaaaaggca ccgttcttaa ttctgatatt tcatcgatga tctcccgtct gggacatacc
    10321 gatacgctgg tggtgtgtga tgctggttta cccatcccca aaagtacaac gcgtatcgat
    10381 atggcattaa cccagggtgt accttctttt atgcaggtgc tgggcgtcgt cacaaatgaa
    10441 atgcaggtcg aggctgccat tatcgcggaa gagatcaaac aacataatcc gcaactccac
    10501 gaaacgttgc tcactcacct tgagcagctg caaaaacacc agggaaatac cattgaaatt
    10561 cgttacacca cgcatgaaca attcaaacaa caaaccgcag aaagtcaggc ggtaattcgc
    10621 agcggagaat gttctccgta tgcgaatatc attctctgtg ctggcgtgac gttctgaggc
    10681 cgtcatggaa gcattacttc agcttaaagg catcgataaa gccttcccgg gcgtaaaagc
    10741 cctctcgggc gcagcgttaa atgtctatcc gggccgcgtg atggcgctgg tgggcgaaaa
    10801 cggcgcgggt aaatccacca tgatgaaagt gcttactggt atctatgctc gcgatgccgg
    10861 cacgctttta tggctgggga aagaaacgac atttaccggg ccgaaatctt cccaggaagc
    10921 cgggattggg attatccatc aggaactgaa cctgatcccg cagttgacca ttgccgaaaa
    10981 cattttcctc ggtcgtgagt ttgttaatcg ctttggcaaa attgactgga aaaccatgta
    11041 tgccgaagcg gataaattgc tggctaaact taacctgcgc tttaaaagcg acaagctggt
    11101 gggcgatctt tccatcggtg accagcaaat ggttgaaatc gccaaagtgc tgagctttga
    11161 gtcgaaagtc atcattatgg atgaaccgac cgatgcgctg accgataccg aaaccgaatc
    11221 cctgttccgc gtcatacgcg agctgaaatc gcaaggccgc ggtattgtct atatctccca
    11281 ccgcatgaaa gaaatcttcg agatttgcga tgacgttacc gtttttcgtg acgggcaatt
    11341 tattgctgag cgcgaagtgg catcactgac cgaagattcg ctgattgaga tgatggtggg
    11401 ccgcaagcta gaagatcaat atccacacct ggacaaagcg ccgggagata tccgcctgaa
    11461 agtcgataat ctctgcggac ctggcgttaa cgatgtctct tttactttac gcaaaggcga
    11521 aattcttggc gtctctggtt tgatgggcgc aggtcgtacc gaactgatga aagtgctcta
    11581 cggcgcacta ccgcgcacca gcggttacgt caccctggat gggcatgaag tcgttacccg
    11641 ttcaccgcag gatggcctgg caagcggcat tgtgtatatc tccgaagacc gtaaacgtga
    11701 cggtttagtg ttgggcatgt cagtaaaaga gaacatgtcg ctgacagcgc tgcgctactt
    11761 cagccgcgct ggcggcagtt tgaagcatgc cgatgaacag caggctgtga gtgatttcat
    11821 tcgtctgttt aatgtgaaaa caccgtcgat ggaacaggca attggtctgc tttccggtgg
    11881 caatcagcaa aaagtggcga ttgcccgcgg tctgatgaca cgccccaaag tgttgatcct
    11941 tgatgaacct acccgtggcg tagatgtcgg cgcgaaaaaa gagatctatc aactgattaa
    12001 ccagttcaaa gccgatggct tgagcatcat tctggtgtca tcggagatgc cagaagtatt
    12061 aggcatgagc gatcgcatca tcgtcatgca tgaagggcat ctcagcgggg aatttactcg
    12121 tgagcaggcc acccaggaag tgttaatggc tgccgctgtg ggcaagctta atcgcgtgaa
    12181 tcaggagtaa aaaaatgaca acccagactg tctctggtcg ccgttatttc acgaaagcgt
    12241 ggctgatgga gcagaaatcg cttatcgctc tgctggtgct gatcgcgatt gtctcgacgt
    12301 taagcccgaa ctttttcacc atcaataact tattcaatat tctccagcaa acctcggtga
    12361 acgccattat ggcggtcggg atgacgctgg tgatcctgac gtcgggcatc gacttgtctg
    12421 taggttctct gttggcgcta accggcgcag ttgctgcatc tatcgtcggc attgaagtca
    12481 atgcgctggt ggctgtcgct gctgctctcg cgttaggtgc cgcaattggt gcggtaaccg
    12541 gggtgattgt agcgaaaggt cgcgttcagg cgtttatcgc tacgctggtt atgatgcttt
    12601 tactgcgcgg cgtgaccatg gtttatacca acggtagccc agtgaatacc ggctttactg
    12661 agaacgccga tctgtttggc tggtttggta ttggtcgtcc gctgggcgta ccgacgccag
    12721 tctggatcat ggggattgtc ttcctcgcgg cctggtacat gctgcatcac acgcgtctgg
    12781 ggcgttacat ctacgcgctg ggcggcaacg aagcggcaac gcgtctttct ggtatcaaca
    12841 tcaataaaat caaaatcatc gtctattctc tttgtggtct gctggcatcg ctggccggga
    12901 tcattgaagt ggcgcgtctc tcctccgcac aacccacggc ggggactggc tatgagctgg
    12961 atgctattgc tgcggtggtt ctgggcggta cgagtctggc gggcggaaaa ggtcgcattg
    13021 ttgggacgtt gatcggcgca ttaattcttg gcttccttaa taatggattg aatttgttag
    13081 gtgtttcctc ctattaccag atgatcgtca aagcggtggt gattttgctg gcggtgctgg
    13141 tagacaacaa aaagcagtaa taacgactac aggacatctt gaatatgaac atgaaaaaac
    13201 tggctaccct ggtttccgct gttgcgctaa gcgccaccgt cagtgcgaat gcgatggcaa
    13261 aagacaccat cgcgctggtg gtctccacgc ttaacaaccc gttctttgta tcgctgaaag
    13321 atggcgcgca gaaagaggcg gataagcttg gctataacct ggtggtgctg gactcccaga
    13381 acaacccggc gaaagagctg gcgaacgtgc aggacttaac cgttcgcggc accaaaattc
    13441 tgctgattaa cccgaccgac tccgacgcag taggtaatgc tgtgaagatg gctaaccagg
    13501 cgaacatccc ggttatcact cttgaccgcc aggcaacgaa aggtgaagtg gtgagccaca
    13561 ttgcttctga taacgtactg ggtggcaaaa tcgccggtga ttacatcgcg aagaaagcgg
    13621 gtgaaggtgc caaagttatc gagctgcaag gcattgctgg tacatccgca gcccgtgaac
    13681 gtggcgaagg cttccagcag gccgttgctg ctcacaagtt taatgttctt gccagccagc
    13741 cagcagattt tgatcgcact aaaggtttga acgtaatgca gaacctgttg accgctcatc
    13801 cggatgttca ggctgtattc gcgcagaatg atgaaatggc gctgggcgcg ctgcgcgcac
    13861 tgcaaactgc cggtaaatcg gatgtgatgg tcgtcggatt tgacggtaca ccggatggcg
    13921 aaaaagcggt gaatgatggc aaactagcag cgactatcgc tcagctaccc gatcagattg
    13981 gcgcgaaagg cgtcgaaacc gcagataaag tgctgaaagg cgagaaagtt caggctaagt
    14041 atccggttga tctgaaactg gttgttaagc agtagtttta atcaggttgt atgacctgat
    14101 ggtgacataa atacgtcatc gacagatgaa cgtgtaatat aaagaaaagc agggcacgcg
    14161 ccaccctaac acggtggcgc attttatgga catcccgaat atgcaaaacg caggcagcct
    14221 cgttgttctt ggcagcatta atgctgacca cattcttaat cttcaatctt ttcctactcc
    14281 aggcgaaacc gtaaccggta accactatca ggttgcattt ggcggcaaag gcgcgaatca
    14341 ggctgtggct gctgggcgta gcggtgcgaa tatcgcgttt attgcctgta cgggtgatga
    14401 cagcattggt gagagcgttc gccagcagct cgccactgat aacattgata ttactccggt
    14461 cagcgtgatc aaaggcgaat caacaggtgt ggcgctgatt tttgttaatg gcgaaggtga
    14521 gaatgtcatc ggtattcatg ccggcgctaa tgctgccctt tccccggcgc tggtggaagc
    14581 gcaacgtgag cgtattgcca acgcgtcagc attattaatg cagctggaat caccactcga
    14641 aagtgtgatg gcagcggcga aaatcgccca tcaaaataag actatcgttg cgcttaaccc
    14701 ggctccggct cgcgaacttc ctgacgaact gctggcgctg gtggacatta ttacgccaaa
    14761 cgaaacggaa gcagaaaagc tcaccggtat tcgtgttgaa aatgatgaag atgcagcgaa
    14821 ggcggcgcag gtactgcatg aaaaaggtat ccgtactgta ctgattactt taggaagtcg
    14881 tggtgtatgg gctagcgtga atggtgaagg tcagcgcgtt cctggattcc gggtgcaggc
    14941 tgtcgatacc attgctgccg gagatacctt taacggcgcg ttaatcacgg cgttgctgga
    15001 agaaaaacca ttgccagagg cgattcggtt tgcccatgct gccgctgcga ttgccgtaac
    15061 acgtaaaggc gcacaacctt ccgtaccgtg gcgtgaagag atcgacgcat ttttagacag
    15121 gcagaggtga cgcttggcta caatgaaaga tgttgcccgc ctggcgggcg tttctacctc
    15181 aacagtttct cacgttatca ataaagatcg cttcgtcagt gaagcgatta ccgccaaagt
    15241 tgaagcggcg attaaagaac tcaattacgc gccatcagcc ctggcgcgta gcctcaaact
    15301 caatcaaaca cataccattg gcatgttgat cactgccagt accaatcctt tctattcaga
    15361 actggtgcgt ggcgttgaac gcagctgctt cgaacgcggt tatagtctcg tcctttgcaa
    15421 taccgaaggc gatgaacagc ggatgaatcg caatctggaa acgctgatgc aaaaacgcgt
    15481 tgatggcttg ctgttactgt gcaccgaaac gcatcaacct tcgcgtgaaa tcatgcaacg
    15541 ttatccgaca gtgcctactg tgatgatgga ctgggctccg ttcgatggcg acagcgatct
    15601 tattcaggat aactcgttgc tgggcggaga cttagcaacg caatatctga tcgataaagg
    15661 tcatacccgt atcgcctgta ttaccggtcc gctggataaa actccggcgc gcctgcggtt
    15721 ggaaggttat cgggcggcga tgaaacgtgc gggtctcaac attcctgatg gctatgaagt
    15781 cactggtgat tttgaattta acggcgggtt tgacgccatg cgccaactgt tatcacatcc
    15841 gctgcgtcct caggccgtct ttaccggaaa tgacgctatg gctgttggcg tttaccaggc
    15901 gttatatcag gcagagttac aggttccgca ggatatcgcg gtgattggct atgacgatat
    15961 cgaactggca agctttatga cgccaccatt aaccactatc caccaaccga aagatgaact
    16021 gggggagctg gcgattgatg tactcatcca tcggataacc cagccgaccc ttcagcaaca
    16081 acgattacaa cttactccga ttctgatgga acgcggttcg gcttagattt acgctgtctt
    16141 ttgatcaaat tattaccatc ggttgttttc agaagcatga acattgctgc tgaagcaaca
    16201 gtaataatgc ccatcgtgat aaacgtatag tggaattgtt cgacagtcgt tgtgccttcc
    16261 atcccttcat aaacgcgaag gacggccgca cttacagcaa cgcctaaact aatcgacagt
    16321 tgctgcgtga ccgccagaac actgttaccg ctgctggcgt tgtcatcggt cagatcggca
    16381 agtgtgatgg tattcatcgc ggtaaattgc gtcgacatag ccatccctaa tataaacaac
    16441 ggcaagatca gcatccatat cgccattgcc ggtgattgca aagagaactg agcgatcatt
    16501 agcccaataa tcaccgtgat ccccactaac gtatggcgat agcccagacg acgtaagact
    16561 tgggtaacca tcgattttgc aataatggaa cctaacgctg tcggtgccat catacagccg
    16621 gcaataaacg cctgataacc aaatcctacc tgtaacatca atggcataag gaacggtaca
    16681 caaccggtcc ccagacgggt tgcaatattg cctacgatac cgatcgagaa agtgcgggtt
    16741 ttaaataaat ctaatgaaat taatgggttt ggcgtgtgtc gcgcatggag aatatagaga
    16801 agcagtaacc cgatgctggt gacaattacc gtcaaggcaa tccagctggc gacaatcttt
    16861 tccccgaata gctctattcc gcttgagaag agaacaaggc tgaggccaaa cagcaaaaag
    16921 ccagtgatat cgaatctgcg tcgtgcggtg gtgaaattgg gcatatgttt gcgcgcgtaa
    16981 agaaggcccg caatacctat ggggatattg attaaaaata tccagtgcca ggttgcccag
    17041 gtgaccagca cgccgccaag aacggggcct aaaattggcc ccaccagacc cggcatggcg
    17101 acaaaattca atactggaag aagttcatta cgaggataag cgcgcagtaa ggccagccga
    17161 gcaacaggca tcatcattgc gccgcctatc ccctgaataa cccggaagac aaccagctgt
    17221 ggtagcgaat tagaaagtgc gcaggccaga gaacccaatg tgaacagact cacggcaagg
    17281 gtaaaaatgc gacgcgtacc gaagcgatcg gctagccatc cgcttaccgg aataagcatc
    17341 gccaccgtca gcgtataact gatgatggct gattgcatcg cgagaggaga acgattaagg
    17401 ctatgagcga ttgcgggtaa ggcggtattc agaatagtgg catcaagtgc ctgcatgaag
    17461 aaggccatcg ccgcgatcca cggcaaaccc gccatactgc gattcttttt atcgctcatt
    17521 caatgtcctg ttatcgggtt gtcacttatc aggtgagcgt agcagcgcct gacaagcttt
    17581 aaatgccgcg tcgccatcgc tttggacaat cgcatcgaca atcgcctggt gcagatccag
    17641 ctttatcact gtgtcgctgg taattgacgt gaagtaagtg tgataaaccg aatggaatag
    17701 cgaggcgaat gaggtcaaaa acggattggc gctcatttca tagatatgct catgccaggc
    17761 catatcgact tcgatccagc gttcacggcg aaagttctct tttaatgccg ccatttcggc
    17821 cattaacgta ttgagatgcg ccttctgttc cgcggtgcca accgttgcag ccagtaggca
    17881 agcttgcggc tccagacaga tacgcataac cagaaagtga tcgatgacct gatgaaagtt
    17941 ctcttctgtc atccaccagg taagcaattc ctgatcaaga aaattccagt ttgattgtgg
    18001 catgacccgg gtaccaattc gcggtcgcgg taaaaccatc ccttttgccg ttaacgtttt
    18061 gaccgcttcg cgtaccgctg tacgactcac tccaaattgc tcgcccagct caatctcacc
    18121 aggcaaaatg gtgccgggtt catattcacc ttttaagatg cgttgcgcca gcttctcagc
    18181 cagaacatac gaaaggtttt tctgtgcagc taactgttgt gcgcttaaag gcattactaa
    18241 tcttcctttt tctttttatt cctccttagt atgccaccag gaagtgtgat tacggttgca
    18301 aaaacggcaa attgcttgtt ttatggcaca ttaacgggga ttttgct
//
LOCUS       DAZFQD010000107        18346 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.107, whole genome shotgun sequence.
ACCESSION   DAZFQD010000107 DAZFQD010000000
VERSION     DAZFQD010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18346
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.107"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(56..286)
                     /locus_tag="ACHKTW_003642"
     CDS             complement(56..286)
                     /locus_tag="ACHKTW_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001171356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6464584.1"
                     /translation="MQKNIRCNCDGLQLALMVQHEFWSTYDPEDRTTAPSKKQVVDFL
                     VSRGASRNLAVSIDKVVRPASMKIGGRPKKWR"
     gene            complement(385..1626)
                     /locus_tag="ACHKTW_003643"
     CDS             complement(385..1626)
                     /locus_tag="ACHKTW_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000101915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase domain-containing protein"
                     /protein_id="HFO6464585.1"
                     /translation="MARKTHPLTTVQIKAARPAQKEYTLQDGGGLFLLVKPSGSKLWR
                     FSYYRPSDKKRILLSFGSLEDVSLADARKRRSEYRTLISAGTDPQDHERQKRETEARR
                     QGNTFENVAAAWYQVKISQNLAPNTIKDIWRSLDKYVFPFIGNTPIDTLTARRFVEVL
                     TPIKERGNLETLKRVLQRVNEVMDYAANSGLIDANPAMNVRKAFPSPVKKHMPTIRPE
                     QLPELMQALSVSATERQTRLLIEWQLLTVTRPAEASSTRWDEINLDAKQWTIPAGRMK
                     MRRDHVIPLSGQAMAVLEAMKPISHHRNYVFPSLKDPQQPMNSQTANAALRRMGFAGV
                     LVSHGLRAIFSTAANEEGFEPDVIEAALAHVDTNEVRRAYNRSNYIEKRIVLMRWWGE
                     FVEAAATGVTLASGKRGIRAV"
     gene            complement(1845..1921)
                     /locus_tag="ACHKTW_003644"
     tRNA            complement(1845..1921)
                     /locus_tag="ACHKTW_003644"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1885..1887),aa:Pro,seq:ggg)
     gene            complement(1996..3756)
                     /gene="yejM"
                     /locus_tag="ACHKTW_003645"
     CDS             complement(1996..3756)
                     /gene="yejM"
                     /locus_tag="ACHKTW_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS biosynthesis-modulating metalloenzyme YejM"
                     /protein_id="HFO6464586.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDEQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQINRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEDEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            complement(3776..4003)
                     /gene="yejL"
                     /locus_tag="ACHKTW_003646"
     CDS             complement(3776..4003)
                     /gene="yejL"
                     /locus_tag="ACHKTW_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HFO6464587.1"
                     /translation="MPQISRYSDEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFARALQSSINEDKAH"
     gene            4185..5192
                     /gene="yejK"
                     /locus_tag="ACHKTW_003647"
     CDS             4185..5192
                     /gene="yejK"
                     /locus_tag="ACHKTW_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009295 - nucleoid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HFO6464588.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            complement(5331..5615)
                     /gene="rplY"
                     /locus_tag="ACHKTW_003648"
     CDS             complement(5331..5615)
                     /gene="rplY"
                     /locus_tag="ACHKTW_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HFO6464589.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(5740..7500)
                     /gene="radD"
                     /locus_tag="ACHKTW_003649"
     CDS             complement(5740..7500)
                     /gene="radD"
                     /locus_tag="ACHKTW_003649"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFO6464590.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAEKRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDTPGAERDVLIEDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHEHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            7649..8344
                     /gene="rsuA"
                     /locus_tag="ACHKTW_003650"
     CDS             7649..8344
                     /gene="rsuA"
                     /locus_tag="ACHKTW_003650"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HFO6464591.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRECIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            8372..9562
                     /gene="bcr"
                     /locus_tag="ACHKTW_003651"
     CDS             8372..9562
                     /gene="bcr"
                     /locus_tag="ACHKTW_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HFO6464592.1"
                     /translation="MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVPA
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALANTIDQLI
                     VMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIAPLMAPIVGGWVLVW
                     LSWHYIFWILALAAILASAMIFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVLSY
                     MLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNIVFLFVMTIFNSRFVRRI
                     GALNMFRSGLWIQFIMAAWMVISALLGLGFWSLVVGVAAFVGCVSMVSSNAMAVILDE
                     FPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCATSSILFCLYASR
                     PKKR"
     gene            9895..10239
                     /gene="yejG"
                     /locus_tag="ACHKTW_003652"
     CDS             9895..10239
                     /gene="yejG"
                     /locus_tag="ACHKTW_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejG family protein"
                     /protein_id="HFO6464593.1"
                     /translation="MTSLQLSIVHRLPQNYRWSAGFAGSKVEPIPQNGPCGDNRLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(10243..11832)
                     /gene="yejF"
                     /locus_tag="ACHKTW_003653"
     CDS             complement(10243..11832)
                     /gene="yejF"
                     /locus_tag="ACHKTW_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HFO6464594.1"
                     /translation="MTQTLLAIEDLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFRGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNNAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LINSQGSIIFDGQPLQNLNRRQLLPIRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFATPQQEYTRQLLALS"
     gene            complement(11834..12857)
                     /gene="yejE"
                     /locus_tag="ACHKTW_003654"
                     /pseudo
     CDS             complement(11834..12857)
                     /gene="yejE"
                     /locus_tag="ACHKTW_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(12857..13951)
                     /gene="yejB"
                     /locus_tag="ACHKTW_003655"
     CDS             complement(12857..13951)
                     /gene="yejB"
                     /locus_tag="ACHKTW_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HFO6464595.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HVFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            complement(13952..15766)
                     /gene="yejA"
                     /locus_tag="ACHKTW_003656"
     CDS             complement(13952..15766)
                     /gene="yejA"
                     /locus_tag="ACHKTW_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416682.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFO6464596.1"
                     /translation="MIVRILLLFIALFTFGVQAQAIKESYAFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFMTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKDHKLSDPLATPPLASGPYRITSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRPVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNEAGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRSRDYDMMPRVWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLLLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKAAKLPSASKQGE"
     gene            complement(15848..17404)
                     /gene="pdeN"
                     /locus_tag="ACHKTW_003657"
     CDS             complement(15848..17404)
                     /gene="pdeN"
                     /locus_tag="ACHKTW_003657"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HFO6464597.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLIIDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVAEGVETPEQARWLSERGVNFMQGYWISRPLPLDDFVR
                     WLKKPYTPQW"
     gene            complement(17585..18151)
                     /gene="mepS"
                     /locus_tag="ACHKTW_003658"
     CDS             complement(17585..18151)
                     /gene="mepS"
                     /locus_tag="ACHKTW_003658"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HFO6464598.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
BASE COUNT         4482 a         4595 c         4707 g         4562 t
ORIGIN      
        1 gatgatttga aatggaatta taaaaaaagc ggcatttctg ccgcttccag gattgttacc
       61 gccatttttt gggcctgcct ccgatcttca tagatgccgg acgtacgacc ttatcaatac
      121 ttaccgccag atttctggaa gcgccacggg ataccagaaa atctactacc tgtttttttg
      181 atggggccgt cgttctgtcc tccggatcgt aggttgacca aaattcatgc tgaaccatca
      241 gggccagttg cagcccgtcg caattgcatc tgatattttt ttgcatctta acggtttcat
      301 gtttcacggc cttgtcgtta aatttgatgc aattatacga cggttttaca tggttttcgt
      361 agtttttact ggttttctgt acagctacac ggctcggata ccccttttac cactggcgag
      421 ggttacgccc gtcgccgcag cctcgacaaa ttcgccccac cagcgcatca gcacgatgcg
      481 tttttctatg tagttgctcc ggttgtatgc ccgtctaact tcgttggtgt cgacgtgggc
      541 aagtgccgcc tctattacgt ccggctcgaa tccttcctcg ttcgctgctg tgctgaatat
      601 ggcgcgtaat ccatgagaca ccagcacgcc agcgaatccc atacgccgca atgctgcgtt
      661 agctgtctgg ctgttcatcg gctgctgtgg gtctttcaga cttgggaaaa cgtaattgcg
      721 gtggtggctg attggtttca tggcctccag caccgccata gcctgaccgg aaagcgggat
      781 aacgtgatcc ctgcgcatct tcatgcgtcc ggcaggtatc gtccattgct tcgcgtccag
      841 gttgatttca tcccaccgcg ttgatgacgc ttcggcggga cgggttacgg tcagtaactg
      901 ccattcaatc agtaatctgg tctgccgttc tgttgccgat actgataaag cctgcataag
      961 ctccggcagc tgttcggggc ggattgttgg catatgtttt tttacaggtg agggaaacgc
     1021 cttacgcaca ttcatagccg gattggcatc aatcagccca ctgttggcgg cgtaatccat
     1081 tacctcatta acgcgctgta aaacccgctt gagtgtttcc aggttgccgc gctccttgat
     1141 gggggtaagc acttcaacga accttcgggc ggtgagggta tctattggcg tgttgccgat
     1201 gaacgggaat acatatttat ccagcgaacg ccagatgtct ttaatcgtgt tgggggccag
     1261 attctggctg attttcacct ggtaccatgc cgccgccaca ttttcgaacg tgttcccttg
     1321 tcttcgggcc tctgtctctc ttttttgcct ctcgtggtcc tgcgggtcag ttccggcact
     1381 gattaacgtc ctgtactcgc tacggcgttt tctggcatca gccagggaaa catcttcaag
     1441 cgatccaaaa ctcagcaata ttcttttttt gtccgatggt cggtagtagg aaaatctcca
     1501 gagttttgat ccagacggtt tgaccaggag aaaaagccct ccgccgtcct gcagggtgta
     1561 ctccttttgc gctggtctgg ctgctttgat ctgcactgtg gttaatgggt gtgtttttct
     1621 cgccatatgt ctatgattcc acacgaaaaa acagggtttt agttatacgg ctttagttat
     1681 acgccagccc gtataactaa acgtataact aaaaaatccg gctgatagct tataaatccg
     1741 gtgatgttcg gtgactaaaa aacataaaaa aaaccgccaa tcaagtgatt agcgggttat
     1801 ccctgtctta tccggtgctt tccggtgata tccggtgggc gatttggtcg gcacgagagg
     1861 atttgaacct ccgacccccg acaccccatg acggtgcgct accaggctgc gctacgtgcc
     1921 gactcgtggc tgctaatact accgttttcc acaccgattg caagtaagat attttgctaa
     1981 ctgatttata attaatcagt tagcgataaa acgcttctcg tctgtcagca cttgcaataa
     2041 caaacttaac tgtggttttt catctttcac tctttcacca cgtaagttgt aagtgcggta
     2101 tttaccgtta ttgttcagca ccagcgtctt tttcggggtg gtaattgcca gcgtatcgtt
     2161 atccgctgca gtaacccagt aatgacggcg ttgagggttg aacaaatcct gaccttgcga
     2221 atattcgctg gcaggtgtgc tgacatgtag caggcgttgc atcagcgtcg tcatcagatc
     2281 ggtatgatca gtcagagcat taatacgctg cgccggcgtg cctggccagt gaatcactaa
     2341 tggcacctgc agatgaccgt gggaccagtc aaaggtttct tcgtcttcgc tcagcggaat
     2401 accccgaccg gcagtgataa tcaccaccgt attgtccagt ttgccagaat cacgcagtgc
     2461 attgagcacg cggttgatct ggtcatcgac attgcctgcc gcccggctat atttccgtgc
     2521 aaatgcctgc tgattgctgt cgtcaatgtt agtgccattg aaagagaccc acgagaacca
     2581 gcggttatct tcttgtgcgt agcggcccag ccagttgatc cactgcgtgg cggtctgctc
     2641 gtcggattgg gtgcgtacgc tcggcatcga gaaatctgac aacaatgcct ggcgatacag
     2701 cgggctggta aagccatctg atgagaataa tcccagctga tagccttgct gattaagcgc
     2761 agtaattaac gccgcagggg tacgggtcga cagaatgccg tccatatagc tcggcgagat
     2821 gccatagaac aggccaaaga tgccgttgtc tgtagtgttg ccggagctca tatggcgcgt
     2881 gaacgaaata ttttgctcag caaaacctgc cagcgcaggc atctgcttct cgaagcgtga
     2941 gtagttcagg ccatcgacag taatcaacag cacattctgc ccggtgccca tatcgcgata
     3001 gcgcagttcg cttaacggat actgaacgga aacggcgtct ggattacctt gctcaataag
     3061 acggcgttga tactcctgcg catcaagcag accatgcttc tcaagaaaac gtcgcgccgt
     3121 catcgggtac gaaagcggca ggttagcgcg ctgcatggtg atcgggcgat agaagttggc
     3181 atcggcccag atatacacca catgcgaggc gataaaggcg ataaataaga atgcggccag
     3241 cgggcgcgcg aagcgccgac gacgcgtcag gctgcgcagc ttttgccagc tccacgtcgc
     3301 aaacaccagt tcaagcaata aaataaccgg cacgctgatg aacatcagct gccagtcgcg
     3361 cgccatctca ttttcgtctg ggttgataac cagttgccag acgatgggat taagatggag
     3421 atggaaacga gtaaagactt cgctatcgat cagtaatagc gtcattcccg ccgttgccag
     3481 aatgacggac aaaaacctca tcagcctctg ggagccgacg ataaaggtca gcgggaagag
     3541 gatcagcaag taggtggcga acaccaggaa gctgaaatgg ccgataatgc ttacgtagga
     3601 ataaatgcga ccagcaagcg ttgtcggcca gtcggcgata aacaggtaac ggctgccaat
     3661 gacgagcgaa agcagaatat tgaacagtgc aaaccagtgc ccccaactga ccatctggga
     3721 gactttttca cggtagcgct gacgatgagt taccataacc tgttatctgt ttcccttagt
     3781 gcgctttgtc ttcgttgata gaggactgta aggcgcgggc aaaagagttg gcaattgcct
     3841 ggcgttgggc cggggcaatg ctggtattga taaggttagt gaccatgttg cctaacacca
     3901 tcagggaaag gtcggtcgga gccttatgtt tttccagtac gttgagcagc tctgcgagca
     3961 attgttcaac ctgttcatca ctgtagcggg aaatttgtgg cataaatcga aatcagttag
     4021 ttgatgaaag ggcaacatat taccgtagca acggtttttt ttctgctttt ttatgcgtta
     4081 aatcattctc gttacgctgc cttctgtaaa atgaccgcca tcttttattg cgtcgcttaa
     4141 ccggcggtgg ttgaataccg cccggtctta aaggagagtt tatcatgagt ctggatatca
     4201 accagattgc tctgcaccag cttatcaagc gtgatgagca gaaccttgag ctggtgttgc
     4261 gcgattcatt gctggaaccg acagaaaccg tcgttgagat ggtggcagaa ctgcatcggg
     4321 tctatagcgc caaaaataaa gcttacggct tgtttagcga agagagtgaa ctggcgcaga
     4381 cgctgcgctt acagcgtcag ggcgaagagg actttctggc atttagccgc gcggcaaccg
     4441 ggcgcctgcg tgacgaatta gcgaaatatc cttttgctga cggtggattc gtgctgtttt
     4501 gccactaccg ttatctggcg gtggagtatc tgctggtggc agttctgagc aacctgagca
     4561 gtatgcgcgt taacgaaaat ctcgacatca atccaaccca ttatcttgat atcaatcatg
     4621 cggatattgt tgcgcgtatc gatttaaccg aatgggaaac caatccagag tccacccgct
     4681 atctcacttt cctgaaaggg cgagtagggc gcaaagtggc ggatttcttt atggatttcc
     4741 tcggggccag cgaagggtta aacgcgaaag cgcaaaaccg tgggctgttg caagcggttg
     4801 atgatttcac tgccgaagca caactggata aagcagagcg acagaacgtg cgccagcagg
     4861 tttatagcta ctgtaatgag cagctgcagg caggcgaaga gatcgaactg gagtcgctgt
     4921 cgaaagagct ggcaggcgtc agcgaagtga gtttcacgga gtttgcggct gaaaaaggct
     4981 acgaactgga agagagcttc ccggcagacc gtagcacact gcgtcagttg acgaagtttg
     5041 ccggtagcgg cggtggcttg actattaact ttgatgcgat gctgttaggt gaacgcatct
     5101 tctgggatcc ggcaaccgat accctgacca ttaaaggcac gccgccgaat ttgcgtgacc
     5161 aattgcagcg ccggacgtct ggcggcaatt aatcggtttt gtgttctgaa tgccagatgc
     5221 ggcgtgaacg ccttatctgg cctgcaaaaa atagcaatac cgtaatcagg cataatctgc
     5281 agatacaaaa aaccccgccg gagcggggtt ttttacaact tattcagcaa ttaagcgcga
     5341 acgaagtcga tgtgctgcag cttcggtttg tacgggtgac gctgtacgtc ctgagcttta
     5401 actttgattt ctttaccgtc aacaacgatg gtcagaactt cgctgtagaa ttcagcttta
     5461 gcttgcatgt tcatgacttt gtcgtgatcc agctcgatag ccagcggcgc ttctttgcca
     5521 ccgtagatga ttgccgggaa cttgttagcg gcacgcaggc ggcggctcgc acccttaccc
     5581 tgctctttac gtacttctgc gttgatagta aacatttctt tctctcttaa attaatcctg
     5641 ctacaggcga cccagcaaca ggtaagtgat ctgctttgcg tatgcaaaag cgggcgagat
     5701 tctatactca atcgctttat acatcaatca aaactaccat taaccgcgta attcgtgcgc
     5761 ccgacgaaaa cgaccttcgt aatcgaacac tttttcacgc acttgccagt actgaccttt
     5821 catgcgggca acgacaaaat ccgggtgtcg caataaggct tgctgggcga ggatatcggc
     5881 ggcggtgatc cagcgcagag ggatgcccgg tgtgcgcgta tgcgggcgga taaaaagctg
     5941 ctcgaaggca gttcgctggg cgggcgtttg cagacggaaa cgctcactca catccgcgcc
     6001 gtcttcatcg taataggtga ttttcaacca ttcgcctttc tcgtcgtgct catgctgcaa
     6061 agacatgccg ctacagcgta ataccagcgc atctttcagt cgtagcgccg cttttaacat
     6121 atcatccgga tcaaccagta ccgtgtcaca ttcgcggcag cggcgagcgg caatatcatt
     6181 ttctgcgtta cattgcgggc aatttttaaa acggaaacgg aagtcacact gttcgcgatg
     6241 accgtcgtca tcttcaaacc atccctgaca gcggcgacca aagtgttcaa tcaatgtccc
     6301 gtcggcggtc gttttccccc aaaaggtgtt ggcaaaaccg caggcagggc agaaaacctg
     6361 taccggaacg ttgtcacttt tgcctttcgg tgtaccaact tccggcgcgt agagatcgtg
     6421 aggattaccc gcataatcaa gaattaaaca atcagtcttg cccggagcga gacgcagacc
     6481 gcgcccgaca atttgttggt aaagactcac tgattcggta gggcgcagaa tggcgataag
     6541 atcgacgtgc ggggcgtcaa atccggtggt cagtactgcg acgttgacca gatagcgaaa
     6601 acgctgggct ttaaaatctt caattaacac atcgcgctca gcgccggggg tgtcgccagt
     6661 aatcagtgca gcatcttcgg caggcagtaa tcccacaatc tcttttgcgt gttcaacggt
     6721 ggcggcgaaa atcattaccc ctttgcgctt ttcggcgaac tccatgatct ggctgatgat
     6781 atgtggcgta atgcgttgct gttttttcag ctcacggttg agatcagctt cgctgaacag
     6841 cccgttactc tgtgcctgca agcggctgaa atcgtattgc actactggca tatccagtcg
     6901 ttctggcggc gtcagatagc cgtgtttaat catataacgc agcggcagct cataaatgca
     6961 gtcacggaaa agggctttct catcgccgcg taccatgccg tgataatgga actggtagat
     7021 ccagcctttg cccaatcgaa aaggcgtggc agtcagcccc agcaggcgta agtggggatt
     7081 cactttagtc aggtgagtga ggatttgctg atactggctc tcttcatcgt caccaatacg
     7141 gtggcattca tcgacaatca acagcgaaaa ttcaccctga aaggcatcaa gattacgagc
     7201 gaccgactgc acgctgccaa ataccacttt accgtggctc tctttgcgct ttaacccagc
     7261 ggcaaaaata tcggcttcca gccccagcgc ctgatacttt gcatggtttt gcgccaccag
     7321 ttctttgacg tgtgccagca ccagaacacg accacgcgcc agccgtgcca gttcggcaat
     7381 caccaggctt ttacctgcgc cggtgggcag cacgataacg gcaggggttt tatgacgacg
     7441 aaaatggttg agcgtggcat ccacggcttc ttgctgatat gggcggagtg taaaaatcat
     7501 ggtctcgcgt aggtagatta ttctgctaat agtatgccat ggatcttttt cccttgaggg
     7561 atgttattag accgctatac tgggcagaca acagttcaga ttttcgggtc aatgcccgac
     7621 ctccgttttt acaggcaaaa tcacacacat gcgacttgat aaatttatcg cacagcaact
     7681 cggcgttagc cgtgctattg ccgggcgtga aatccgcggc aatcgtgtca ccgtcgatgg
     7741 cgaaattgtt cgtaatgcag cgttcaaact gcttcctgaa catgatgtcg cttacgatgg
     7801 caacccgctg gcgcagcaac atggtccacg ttacttcatg ctcaataagc ctcagggcta
     7861 tgtttgctcc acggacgacc ctgatcaccc aacggtgctc tattttctgg atgaaccggt
     7921 agcctggaaa ctgcacgccg caggacgtct ggatatcgat accaccggtc tggtgctgat
     7981 gactgatgat ggtcagtggt cgcaccgcat tacttctccg cgccatcatt gcgagaagac
     8041 ctatctggtg acactggaat cacctgtagc tgacgatacg gcagagcaat ttgctaaagg
     8101 cgtgcagctg cataacgaaa aagatctcac taagcctgcg gtgctggaag tgattacccc
     8161 aacgcaggtt cgtctgacca tcagcgaagg gcgttatcat caggtgaaac gcatgttcgc
     8221 cgccgtgggt aaccacgtgg ttgagctgca tcgtgaatgt attggcggta ttacgctgga
     8281 tgctgattta gcccccggtg aatatcgtcc gttaactgaa gaagaaattg ccagcgtcgt
     8341 ctaacttttc catgactttc aggagcccgt tgtgaccacc cgacagcatt cgtcgtttgc
     8401 tattgttttt atccttggcc tgctggccat gttgatgccg ctgtcgattg atatgtatct
     8461 gcccgcgcta ccggtaattt cagcgcagtt tggcgtacct gcgggcagta cgcagatgac
     8521 cctcagtact tatattctgg gctttgcgtt ggggcagtta atctacgggc cgatggcaga
     8581 cagcttcggg cgtaagccgg tggtgctcgg cggtacgctg gtgtttgccg ccgccgcggt
     8641 ggcgtgtgcg ttggcaaaca ccatcgatca gctgattgtg atgcgtttct tccacgggct
     8701 ggcggcggct gcggccagcg tggttatcaa cgctttgatg cgcgatattt acccgaaaga
     8761 agagttctcg cggatgatgt cgtttgtcat gctggtgacc actattgcac cgttgatggc
     8821 accgattgtc ggcggttggg tgctggtgtg gctgagctgg cattacatct tctggatcct
     8881 ggcattagcg gcgattctgg cttcggcaat gattttcttc ctgattaaag aaaccttacc
     8941 accggagcgt cgtcagccat ttcacattcg taccactatt ggtaactttg cggcgctgtt
     9001 ccgtcataag cgtgtcctga gttatatgct ggccagcggt ttcagctttg ccggaatgtt
     9061 ctcgttcctg agcgccggac cgtttgtcta cattgaaatt aaccacgtcg cgccggaaaa
     9121 ctttggttat tactttgcac taaacattgt tttcctgttc gtgatgacca tctttaacag
     9181 ccgcttcgtt cgccgcattg gcgcgttaaa tatgttccgc tcggggttgt ggatacaatt
     9241 tattatggca gcgtggatgg tcatcagtgc gctgctgggg ctgggattct ggtcgctggt
     9301 ggttggcgtt gcggcgtttg tgggctgcgt gtcgatggtg tcatccaatg cgatggcggt
     9361 cattcttgat gagtttcccc atatggcggg aacggcatct tcgctggcag gaaccttccg
     9421 ttttggcata ggggcaattg ttggcgcatt gctttctctt gcgaccttta actctgcatg
     9481 gccgatgatt tggtcaattg cattctgcgc aaccagctcc attctcttct gtctgtacgc
     9541 cagtcggccg aaaaaacggt gatctattgc acaacgagga aggtaaaagg cttcctttgt
     9601 tgatgcatgt caaccacaaa tctatcattt ccccgatata tgtttatttt atgtaaaatc
     9661 aatttatgta aaaagtcaca tcattgtagt taaaaaggtt gagttagatc gcagaaacgg
     9721 gtacatatag ccccgcaaac gtgaccacgc ccgcagatat tacttaaatc agagccatag
     9781 aggccacgca ggcgaggcat caatctttac gatctgtata aagacggatt gttgatgatg
     9841 tgttaaaatt gatgtaaaca aattgtgaag tgaatgtact tccggggaaa ataagtgact
     9901 tcattacaac tctcaatcgt ccatcgactg ccgcagaact atcgctggtc tgctggtttc
     9961 gcaggttcga aggttgaacc gattccgcaa aatggaccgt gcggtgacaa cagactggtg
    10021 gcgcttaaat tgcttagccc ggatggcgat aatgcatggt cggtgatgta taaactaagc
    10081 caggcgttaa gcgacatcga agttccatgt tcggtgctgg agtgtgaagg tgagccgtgc
    10141 ctgtttgtaa atcgccagga cgagtttgct gcaacatgcc gattgaaaaa ttttggtgtg
    10201 gcaattgccg aacccttttc aaactacaat cctttttaag cgtcagctca acgccagtag
    10261 ctgacgcgta tactcctgct gcggtgtggc aaacacgcgc gcgcacggtc cttgttccac
    10321 tacttcccct tgtcgcagta cgataacctg atgacataac gcgcggacaa cgtgcaaatc
    10381 gtggctgata aacaaatagg ccagttgatg cttttgttgc aatgatttca atagcgtcaa
    10441 tatttgcgcc tgtaccgttt tgtcgagtga tgatgtcggt tcatcaagta tgatcagcga
    10501 gggcttaaga attaatgccc tggcaatcgc aatacgttgt cgctgaccac cagagaactc
    10561 cgccggataa cggtggcgtg tttcaggatc taatcccact tcatgcatca cggctatcac
    10621 ttgttgttcg cgttgtgcgg cagaaagcgt cggctggtga acccgtaagc cttcctcaat
    10681 aatctgcaaa acgttgagtc gtgggttgag cgaggagttt ggatcctgaa ataccacctg
    10741 aatgcgatga cgaataggta acagctggcg gcgatttaaa ttttgcagtg gctgaccgtc
    10801 aaagatgatg ctgccctgag aattaatcag tcgcagcagc gccagtcccg tcgtactttt
    10861 cccggaaccg gactcgccca ctaaacccag tgtttcaccc gctcgtagcg taaaactgat
    10921 gtttttcacc accacattat gatccacaat gcgcttcaaa atccctttgc gaatggggaa
    10981 ggcaacctga agctgttcaa catccagcaa cgttgaggca ggttctggca acggcaccgg
    11041 gtcgcctgac ggttcactgt tgagtagctt ttgtgtgtaa ggatgagtgg gtgaggcaaa
    11101 tagcgtagcg gcgttatttt gctcgacaca gcgaccgttt tgcattaccg ccacgcggtg
    11161 ggccagtttt ctgacaatgc tgaggttatg agtaataaac agcatgccca tattcagctc
    11221 gccttgcagt tcgcgcaaca gctgtaaaat ctgcgcctgg acagagacgt ccagtgcggt
    11281 ggtcggttca tcggcaatta ataattccgg tcgcgttaac agcgccatcg caatcatcac
    11341 ccgctggcgt tcgccgccgg agagctgatg cggataatct gtcagccgtt ttgccgcctg
    11401 gcggatacca acgcgatcaa ggcagttaag aatttcgcct cgagccgctt cccgacgcat
    11461 cccgcggtgg agtgaaagca cttcataaag ctgtttttcc agggtatgca atggatttaa
    11521 cgacaccatc ggctcttgaa aaatcatggc gatcttatta ccgcgtacac cgcgcaacgt
    11581 ttgatcgctg gcatgaagca gcgattcgcc acgaaaacga atatcgccgg agagatattc
    11641 aaccggcggg gaagggagca ggcgtaaaat tgacagcgcg gtaacgcttt tgcctgaacc
    11701 tgactcaccc accagcgcca gcgtttcgcc agcctcaatc tgtagtgaaa catcattgac
    11761 tactgtacgt acggtttgct gatggcgaaa acccaccgac aaatcttcaa tcgctaacag
    11821 agtttgcgtc atgctacacc gccttattag gatcaaatgc gtcgcggacg gcttcaccaa
    11881 taaagatcag caaagacaat aatatcgcca ccgacaagaa ggcggtgatc ccaagccacg
    11941 gagcctgaag gttatttttc ccttgtaaca gcagttcacc gagtgacggt gaaccgagcg
    12001 gcagaccgaa gccgaggaaa tcgagcgagg tcagggtggt tatcgaacta cataaaataa
    12061 acggtaaaaa ggtgagggtc gcgaccatgg cattaggcaa catatgacgc aggatgatac
    12121 tgcgatcgct gacgccaagc gcctgtgccg cacgaatgta gtcgaaatta cgagtacgta
    12181 aaaactccgc ccgcaccacg ccgaccagac tcatccagcc aaacaacagt aattgccagc
    12241 agccaccaga agttaggctg tacgacgctg gaaagtaaaa taatcaaaaa cagcgtcggc
    12301 atccccgacc atacttcaat aaagcgttgt ccccagagat cgactttacc gccgtaatag
    12361 ccttgtagcg cccccgccag cacgcccatc acgctggaac agagagtcag catcaggcca
    12421 aacagaaccg agatccgcgt gccatagaga atgcgtgcca gcacatcgcc gccgttggca
    12481 tccgttccca gccagttttg ccgggagggt ggagaaggga agggcttatc ggtagcaaag
    12541 ttgatactgg tagcaccaaa gcgaatcggt gcccacagta cccagccgtt attttccagc
    12601 cgttgtttca gccacgggtc ctgataatca gcctgacttg ccagcgggcc gccaaaatcg
    12661 ctttcgctgt agtttttcaa caacgggaaa taccaactgc cgtcataacg taccagcaac
    12721 ggtttatcgt tggcgataag ttcagaacac aaactcaaac caaacaaaac gaggaaaatc
    12781 cataacgacc agtagccgcg acggttatgg cgaaaacgcg cccaacgggc ctgattgacg
    12841 gggctgagtc gcgacattaa cgtccctcaa aatctatacg cggatcgacc agcgtatagc
    12901 tgatatcact gacaatattc agcagcaggc caatcagggt gaaaatatac aaggtaccaa
    12961 acattacagg ataatcgcgc gagacggtcg cttcgtagcc cagtaagcca aggccattga
    13021 gtgaaaacat cacttcaatc agcagcgagc cggtaaaaaa catgctgata aacgtcgccg
    13081 gaaaaccggc aatcaccagc agcatggcgt tgcggaacac atgtttccag agaatatttt
    13141 tttcacttac ccctttcgca cgcgcggtca ccacgtattg cttgcgcact tcatcaagga
    13201 atgagttttt ggtcagcatg gtcagcgccg caaagccacc aatcactgtc gccagcaccg
    13261 gcagcgtgat atgccacaga taatcggtga ttttctgata ccacggcagc gaatcaaagt
    13321 tagcggaaac caggccgcgt agagggaaca ggtcgaaata gctgccgccc gcgaagaaga
    13381 caatcagcag gatggcaaac aaaaaggccg gaatggcgta gccgatgatg ataaatgcgc
    13441 tactccagac gtcaaagcgg ctcccattat aaacagcttt gcgaatgcct aacggaatcg
    13501 acaccagata gataatcagc gtgctccaca atccgagggt gatggaaacc ggcagactgt
    13561 ctttaatcag cgtcagcacc gaggcgctgc gaaacaggct atcgccaaaa tcaaagcgga
    13621 tgtagtccca gagcattttg aagtaacgtt cgtggatcgg cttatcaaaa ccgtagcgat
    13681 gagtgatctc agcgatcact tctggatcta atccgcgtcc gccacggtaa ttactgtcgc
    13741 tgatattgcc gacacccgtt tgcgcatggc tggcacgaac accttcaccg cctgcgccgg
    13801 gtaatactcc ggcattacca aactcaatgg cggcgatggc ctggtcgacc ggaccgccag
    13861 gcgcaatttg cacgatgaaa aagttgatgg tgataatcgc ccataatgtt gggatcacca
    13921 gcaacagacg gcgaatcagg taagcgccca tctactctcc ctgtttgctg gcggacggca
    13981 gtttggccgc tttattaacg tcataccacc aggtatcgat acccaggctg taaacagggc
    14041 gtacagcggg ttgggagaat ttatcccacc aggcgagacg gtcttccgcc atgtaccaca
    14101 ttggcagcat gtaataattc cacgttaata cgcgatccag tgctcgccct aacagcagta
    14161 atttttcttt atttccctgc gcggcaataa tttgattgat cagcgagtcg ataaccggac
    14221 tttgcacgcc gggggcatta taagtggaat tgatatattc cgatgaccag gaaatctgta
    14281 aatcggaact gggccacggc atcgcccgcc atacgcgcgg catcatgtca tagtcgcgac
    14341 tgcgcatgcg gttggtgatt tgcgagttat ccaccttgcg aatgtccatg ttgataccca
    14401 gccgttgcag gctgtgctgg aacggcaata cccactgact attgctgctt gcgggaagca
    14461 ataattcaaa gctgagtggc tgacccgttg tggcattaac gcgttgctga cccttcagca
    14521 cccagcccgc ttcgttgaga agtttgtcgg cttttaacag gttgtcacga tcgtagccat
    14581 cgcctttaga taccggcggc tggtagattt gtgtgaagac ttctggcggt agatcttttt
    14641 tcattggtgc cagaagcacc agctccgcgg cgtcggggta atttctggcc gcgtattcgg
    14701 tattctgaaa ataactgttc gtgcgactcc aggcattgta aaacaacgcc ttgttcatcc
    14761 attcaaagtc aaaggcgagg gtgattgctt cccggacccg gcgatcgctg aataccggac
    14821 gttggatatt aaacgccagc cagcgcgtat cctgggctga ttcgttcttt tgctcatctt
    14881 tgatgatgta ttttttatcg aaatttttac cggtataacg cgtggcccag tttttagcgt
    14941 cgttttccat acgcaaatca aaggcacctg ctttaaacgc ttcaaaggcg acattatcat
    15001 cgaggtaata atcgtagcga atggtgtcga aattccagcg tccacggttt accggtaagt
    15061 ttgctgccca gtaatctttc acgcgggaat agacaatatt ttgccccatt ttccaggacg
    15121 taatgcggta cggaccactg gcaagcggag gcgtggcgag cgggtcgcta agtttgtgat
    15181 ccttccagta cttttctgga aataccggca gcgaaaacag actcaacata tcttctttgc
    15241 cgggtttagc taactcaatg cgcacggtta acggcgcaat ggctttgacg gtggtgcctt
    15301 tgtagaccag acgaaattgc ggcacgcctt cggtcataaa tttttgaaaa gtaaactcta
    15361 catcgcgggc agtaatgggc gaaccatcat gaaaacgggc gcgcggatta atagcgacct
    15421 ccacccagga ataatcgtca gcatagcgtg cgctttcagc aatcagcggg taataactgc
    15481 ctggttcgtc atcggaagtc gtaaatagcg tgtcgtacag ctgctcggtg cgtgcgcccg
    15541 ggttgccgcg cagtgcatag cggttgaaat tatcgaaggt gccgagggct gacaacgtta
    15601 tctgcccacc ttttggcgcg gcggggttca cataatcaaa atggttgaaa ttaaacgcgt
    15661 accggggttc gcccagcacg gcaaaggcat agctttcctt gatagcctgc gcctgcacac
    15721 caaaggtgaa cagagcgata aacagcagca gtatgcgcac aatcatctgc aacggatttc
    15781 cttttactca tcattagccg caatccggca tgctttcact atgaataata agggacgtaa
    15841 gcacacctta ccactgcggc gtatacggtt tcttcagcca gcggacaaaa tcgtccagcg
    15901 gtaacgggcg actaatccag tagccttgca tgaaattaac gccgcgttcg cttagccatc
    15961 gcgcttgttc cggcgtttcg accccttcag caactgtcag catattgaga cgtttcgcca
    16021 gcgtcagcac cgcgtcaagt acgggtgaag tgatcgtttc cgtaccgatg gcgttgataa
    16081 atccacggtc aattttcaga taatcgagcg taaaacgctc aagatagata agcgcgctgt
    16141 gcccggtgcc gaagtcatca atagcaattt ctacgccgac cgagtgcagc caggcgaaga
    16201 gttgtgtggc ttcttgctct ttcagcatat cgcgctcggt aatttccagc acaatctgga
    16261 aatggtgtgc gggcagggaa gtgagcagtt tctggatatc tgctttaaag ctttcgctgt
    16321 gcagatggtc cggcgcaatg ttaatgccaa atttgacgcc taccggcagt actttttcta
    16381 attctgcggc atcgcgggca attaactcaa acaggtgctg agtcagcggc acaatcatct
    16441 tttgcgattc ggcaaagtta atgaaggcat ccgggggaat ttctcccgcg acaggatgcc
    16501 gccagcgtag cagtacttcc aggcccgtga ctcgtaaagc ttgtgtatcc accaccggtt
    16561 gatacgccac gtaaaattgt tcgcgcttga tggcggtcat gatttctctg ccggggcgca
    16621 tacgtacgct catcaggtaa tagcagagca ggccaacgac agttccactc atgccgccca
    16681 gtaaaaatgc gtaccacaca tcgttccatg tccagtcatc tgcataaagc cgaacggtca
    16741 gaggaatgcc cgcaatttta gtttcacgga ccggcatgtc ggttaattca ttaacgttca
    16801 tcaaacgtga agaaaaggta gatagcgcag tattgccaat aatgagggcg acgccatcgt
    16861 aatcttcctg ccgtgaacta taaaagagtg aaggcgtcag gttgagattc agagcggcaa
    16921 agacgccgct atttttcagc aaagggttgc gataccagat gacgattgcg ggtttgttcg
    16981 gcaccatcgg cgtgccgggt aagatcgcca tatcgacgtt tttattaatg tcgagcgccg
    17041 gaatcaattc attgagtgga atgtccatct caccagtcgc agatgaacag aatgtttttt
    17101 tatctttcac cagcacaaac gtacggacat tcatgctaaa cgccgcgcga gcggtcagtt
    17161 cggggttagc ctgctggcag gtatctaagg tcagcggctg aagccggtca gtagtggatt
    17221 tcaggtcggc aaaataggta tcgagatact tttgtacgtc gataatcagt gtgtcgtatt
    17281 tgacttcgtg cttatgccag gccactaaaa actgaaggca gctaaccagt atcgcaatca
    17341 tcacgcctgc tacaatgcag gtaagcagga gcttacgtcc agaattggga gcgcgtatga
    17401 acatgattcc gttaatttcc tgaaattacg acaacattta aaagataaca atgaccggac
    17461 tatagcctgg atcaagacgc ggaaacatca ttttctctga tgaatgaaaa caaaaaagca
    17521 ctgcctaagc agtgctttca gttaactcgt caggatagcc aagggattgc atccaaacgg
    17581 tttattagct gcggctgaga acccggcgtg cttcgttgta acgcttcttc cagtacggtt
    17641 cattcatgct ggaaataata acaccactgc tggtggaagc atggacaaac tgattgttac
    17701 cgatataaat accgacatgg cgtcccgttg aaccggcacg gaacagaact aaatcacccg
    17761 tacgcaaatt actgcgggaa acagatttac ccatttcctg ctgttcgtaa gtcgaacgtg
    17821 gaagttctaa gccaaattgc tcacggaatg tacgctgtac gaaaccagaa caatcgatac
    17881 cttttttagt gctgccgccc agacgataac gtacgccttt ccagtcagca tactgatcca
    17941 taattcgcga ttttacgtcg acattacgaa ccaggttttc aaattcatcc tgagaagctt
    18001 gcagtgatga tgtttcacta cccactgcac gtgtctcagg atgcatattc tttgcggtgt
    18061 tatttgcact acatgcagaa agcagaaccg ctactgcaat cgcgggaatc ccgcgcaaga
    18121 tatatctcaa aatcggttga gatttgacca tgttgtttgt tttcccttga agtccttaac
    18181 gacaaatatc gttataaaaa aatgccaagc gacacgagac taattacctg ggcatgttga
    18241 aacaattcct taaggccaaa tctgtggact aacgcacaaa attatatgct gtgaggataa
    18301 tctaccgccg aggcaaaacg agttcgagat tacccgattg gtgcgg
//
LOCUS       DAZFQD010000108        18173 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.108, whole genome shotgun sequence.
ACCESSION   DAZFQD010000108 DAZFQD010000000
VERSION     DAZFQD010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18173
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.108"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            279..1226
                     /gene="stxA1a"
                     /locus_tag="ACHKTW_003659"
     CDS             279..1226
                     /gene="stxA1a"
                     /locus_tag="ACHKTW_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx1a subunit A"
                     /protein_id="HFO6464599.1"
                     /translation="MKIIIFRVLTFFFVIFSVNVVAKEFTLDFSTAKTYVDSLNVIRS
                     AIGTPLQTISSGGTSLLMIDSGTGDNLFAVDVRGIDPEEGRFNNLRLIVERNNLYVTG
                     FVNRTNNVFYRFADFSHVTFPGTTAVTLSGDSSYTTLQRVAGISRTGMQINRHSLTTS
                     YLDLMSHSGTSLTQSVARAMLRFVTVTAEALRFRQIQRGFRTTLDDLSGRSYVMTAED
                     VDLTLNWGRLSSVLPDYHGQDSVRVGRISFGSINAILGSVALILNCHHHASRVARMAS
                     DEFPSMCPADGRVRGITHNKILWDSSTLGAILMRRTISS"
     gene            1236..1505
                     /gene="stxB1a"
                     /locus_tag="ACHKTW_003660"
     CDS             1236..1505
                     /gene="stxB1a"
                     /locus_tag="ACHKTW_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx1a subunit B"
                     /protein_id="HFO6464600.1"
                     /translation="MKKTLLIAASLSFFSASALATPDCVTGKVEYTKYNDDDTFTVKV
                     GDKELFTNRWNLQSLLLSAQITGMTVTIKTNACHNGGGFSEVIFR"
     gene            2010..3950
                     /locus_tag="ACHKTW_003661"
     CDS             2010..3950
                     /locus_tag="ACHKTW_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFO6464601.1"
                     /translation="MAFKHYDVVRAASPSDLAERITQKLKEGWQPYGSALISTAGYGA
                     EFIQPVVSEGELPSLAESGNHPHVSAKPEAAPEYYYVIALAGQSNSMSYGEGLPLPET
                     YDRPDPRIKQLARRSTVTPGGAACKYNDIIPADHCLHDVQDMSRLNHPKADLSKGQYG
                     TVGQGLHIAKKLLPFIPANAGILLVPCCRGGSAFTTGADGTYSDAGGASENSTRWGVD
                     KPLYKDLIGRTKAALKKNPKNVLFTVVWMQGEFDFDGTPGNHAAQFGALVDKFRADLT
                     DMAGQCVGGSAGGVPWICGDTTYFWKQKNESTYQTVYGSYKNKTEKNIHFVPFMTDEN
                     GVNVPTNKPEEDPDIPGIGYYGSKWRDSSATWTSQDRASHFSSWARRGIISDRLATAI
                     LRHAGRVALNAGASSTVSEVRPSSPSGAEATGVTTLLSYLASESEGSLKVQGWSASGG
                     RAEVVSDAEGTGGKAVKVTKEAGKSTWVLEYAAGNGAALLQKGGQIRCRFKASGALAA
                     NQYVMAFYWPVSSLPQGVVLTGDGGNNLLAAFYIQTDAKDLNVMYHNAKVATNNLKLG
                     SFGAFDNEWHTLAFRFAGNNSLQVTPVIDGQDGTPFTLTQSPVSAFSADKLHVTDITK
                     SATYPVLIDSIAVEVNNADAAA"
     gene            4086..4265
                     /locus_tag="ACHKTW_003662"
     CDS             4086..4265
                     /locus_tag="ACHKTW_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HFO6464602.1"
                     /translation="MTFLNQLMLYFCTVVCVLYLLSGGYRAMRDFWRRQIDKRAAEKI
                     SASQSAGSKPEEPLV"
     gene            4306..4551
                     /locus_tag="ACHKTW_003663"
     CDS             4306..4551
                     /locus_tag="ACHKTW_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HFO6464603.1"
                     /translation="MSEITSLVTAEAVKDVLRSEEVLSALKQKLRHNLEARLDAEVDA
                     ILDELLGAPAAPEPEGIAGEGSASDSGDPTPDSDMMM"
     gene            4629..4844
                     /locus_tag="ACHKTW_003664"
     CDS             4629..4844
                     /locus_tag="ACHKTW_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFO6464604.1"
                     /translation="MYQMEKITTGLSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTYLTNLYFKIREDRRKAARGE"
     gene            4849..5382
                     /locus_tag="ACHKTW_003665"
     CDS             4849..5382
                     /locus_tag="ACHKTW_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO6464605.1"
                     /translation="MSRKFRYGLSAVVLALIAAGASAPEILDQFLDEKEGNHTTAYRD
                     SAGIWTICRGATRVDGKPVIPGMKLSKEKCDRVNAIERDKALAWVEKNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRINAGDRRGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VSRRDQESALACWGIDR"
     gene            5655..6221
                     /locus_tag="ACHKTW_003666"
     CDS             5655..6221
                     /locus_tag="ACHKTW_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor N-terminal domain-containing
                     protein"
                     /protein_id="HFO6464606.1"
                     /translation="MNMMTVPFHGDSLYVVNHNGEPYVPMKPIVEGMGLAWAPQFTKL
                     KQRFAKGVTEIIIPSAGGEQTMTCLALRKLAAWLQTINSNKVKPEIRDKVIQYQEECD
                     DVLYEYWTKGVVVNPRRMSVMEELNQACADMKRDKNIASVFATGLNEWKQVKAAHVSK
                     IRTLINEANLLIDFVLADTGKGKITKAD"
     gene            6236..6370
                     /locus_tag="ACHKTW_003667"
     CDS             6236..6370
                     /locus_tag="ACHKTW_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001342264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6464607.1"
                     /translation="MISDKLITLAKILCVIVGISFLVMLVAIFFSTAWRVLTLSGLVG
                     "
     gene            6598..6804
                     /locus_tag="ACHKTW_003668"
     CDS             6598..6804
                     /locus_tag="ACHKTW_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1 family lipoprotein"
                     /protein_id="HFO6464608.1"
                     /translation="MRELKMKLCVLMLPLVVSACGSTPPAPVPCVKPPAPPAWAMMPA
                     SNSLQLLDETFSVSETESSATRQH"
     gene            7026..7523
                     /locus_tag="ACHKTW_003669"
     CDS             7026..7523
                     /locus_tag="ACHKTW_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001496757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HFO6464609.1"
                     /translation="MKSLTLFNQPIRVAEDGMICLTDMWKASGKSESESPYHYLRNKQ
                     TKEFLAELEKNHESVVFTERGVHGGTYGGKFVAYDYAAWLNPGFKYAAYKVLDDYFTG
                     ELQHRNSLSAQLNMKCHEFDQKKDMASFCGQGLAAWRYTKPVLVAEINSLANQLQITI
                     PGLPG"
     gene            7520..7777
                     /locus_tag="ACHKTW_003670"
     CDS             7520..7777
                     /locus_tag="ACHKTW_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016063373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6464610.1"
                     /translation="MSDRVIECASRAGRDFSEFMKGEKGMMEALASVDEFGEQLRLNG
                     CVNHHFVSYMMRNSIMQAFMDMAKAERKEERRRKRAEAKAK"
     gene            8177..8983
                     /locus_tag="ACHKTW_003671"
     CDS             8177..8983
                     /locus_tag="ACHKTW_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HFO6464611.1"
                     /translation="MAKLDWKKLEQAFRREHAETGITLLDWCRKKKINYNTARTRIKM
                     GKIDHEIDHKTDHEIDHDISDEEPCNDAGSGDEKCAKNSEKNCANSAETKRIRGSRLL
                     PPSNAFSQRNTHAVRHRGYAKYLEADNLMDDASDMVLFDELVFTRARALSVTKALKGM
                     FADLEEATDVETRVALYDKILKAEQALDRNIARIESIERSLLTLDVLAETAPKLRADR
                     ERINAARDKLRAETDILTSQRRGVVTPVSDIVSSLHEMSNSGRLDDIPEE"
     gene            8964..10670
                     /locus_tag="ACHKTW_003672"
     CDS             8964..10670
                     /locus_tag="ACHKTW_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HFO6464612.1"
                     /translation="MTFRKNEPRCDEPSEMTEAEQRLFIMTKLSNPWWRLNHLYKIQN
                     EKGELVTFRMRPAQRQLFRSMHNKNIILKARQLGFSTAIDIYLLDQALFIPHLKCGIV
                     AQDKQAASEIFRTKIAVPFDHLPDWLRASFTIVERRSGASGGYILFGHGSSIQVATSF
                     RSGTVQRLHISEHGKICAKYPAKAKELRTGTLNAVSDECIIFDESTAEGVGGDFYEMS
                     NRAQEITASGLSLTAQDYKFHFYAWWQDPKYSARVPESGLKLSREKMTYFSAVEKAMN
                     ITLTDEQKQWYINKETEQREEMKQEFPSTPQEAFLTSGRRVFSAESTLQAESFCSPPL
                     IVYDIEPVTGRKTKAQSLRDGNKAEQHRTLMNYLLVWELPDPDEEYVCGADIAEGLEH
                     GDRSSLDIIRCSNGEQVAHWFGHLDAELFAHLIAQVCRMYNNAFVGPERNNHGHAVIL
                     KLRELYPTRYIYNEQHLDQAYDDDTPRLGWLTTRQSKPVLTEGMKTLLNNGISGIRWS
                     GTLSEMNTYVYDAKGSMNAQEGCFDDQLMSYMIAQEMRARMPVRVKQKTDKRRTTHWM
                     AH"
     gene            10670..12814
                     /locus_tag="ACHKTW_003673"
     CDS             10670..12814
                     /locus_tag="ACHKTW_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HFO6464613.1"
                     /translation="MKNETNTMATKNDNGATPRFSQRQLQALCSDIDSQPKWRDAANK
                     ACAYYDGDQLPPEVLQVLKDRGQPMTIHNLIAPTVDGVLGMEAKTRTDLVVMSDEPDD
                     ETEKLAEAINAEFADACRLGNMNKARSDAYAEQIKAGLSWVEVRRNSDPFGPEFRVST
                     VSRNEVFWDWLSRESDLSDCRWLMRRRWMDTDEAKATFPGMAQVIDYAIDDWRGFVDT
                     TITEGQPSPLMSAWEEYQSWDRRENEWLQRERRRVLLQVVYYRTFERLPVIELSNGRV
                     VAFDKNNLMQAVAVASGRVQVKVGRVSRIREAWFVGPHFIVDRPCSAPQGMFPLVPFW
                     GYRKDKTGEPYGLISRAIPAQDEVNFRRIKLTWLLQAKRVIMDEDATQLSDSDLMEQI
                     ERPDGIIKLNPARKNQKSVADVFRVEQDFQVASQQFQVMQESEKLIQDTMGVYSAFLG
                     QDSGATSGVAISNLVEQGATTLAEINDNYQFACQQVGRLLLAYLLDDLKKRRNHAVVI
                     NRDDRQRRQTIVLNAEGDNGELTNDISRLNTHIALAPVQQTPAFKAQLAQRMSEVIQG
                     LPPQVQAVVLDLWVNLLDVPQKQEFVERIRAALGTPKSPDEMTPEEQEVAAQQQALQQ
                     QQAELQMREMAGRVAKLEADAARAHAAAQRDNASAQREVALTQGQRYVDALNQAHTAE
                     IITGVQNMEQEQDVLQQQMLYTLQQRMNEMSL"
     gene            12972..13979
                     /locus_tag="ACHKTW_003674"
     CDS             12972..13979
                     /locus_tag="ACHKTW_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6464614.1"
                     /translation="MDFEFTGEETPEQLEKMLEGLGDVDIDSHAQDVVTEDTTEKHAD
                     EEAQTQTGDNNVAPTPDASVEQTQDVKEPEAKGVLTRDGKHVIPYEVLEAERSGKQRA
                     EQEAALLRGQIAEEKRRVELLTSQIHQAGMKPTPLPENEKISDEQIARIREMYPEIGD
                     AVASLIRKNNYLQSRVQQSTQQAEGNGGEDLSPVLDAMNAVPVLKTWQESDPDRFSVA
                     VSIDGKLQNDPAWKDKTLTERFAEVARRTQVAFGEVSESSADNQADKTDIRKTAEEKV
                     KTAEREQAVPASPSDLGTTASVGTGDNFERLLGASHSEAEAIMRGMTNAEIDALLEKL
                     G"
     gene            14003..15217
                     /locus_tag="ACHKTW_003675"
     CDS             14003..15217
                     /locus_tag="ACHKTW_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019028 - viral capsid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N4-gp56 family major capsid protein"
                     /protein_id="HFO6464615.1"
                     /translation="MTTVTSAQANKLYQVALFTAANRNRSMVNILTEQQEAPKAVSPD
                     KKSTKQTSAGAPVVRITDLNKQAGDEVTFSIMHKLSKRPTMGDERVEGRGEDLSHADF
                     SLKINQGRHLVDAGGRMSQQRTKFNLASSARTLLGTYFNDLQDQCAIVHLAGARGDFV
                     ADDTILPTAEHPEFKKIMINDVLPPTHDRHFFGGDATSFEQIEAADIFSIGLVDNLSL
                     FIDEMAHPLQPVRLSGDELHGEDPYYVLYVTPRQWNDWYTSTSGKDWNQMMVRAVNRA
                     KGFNHPLFKGECAMWRNILVRKYAGMPIRFYQGSKVLVSENNLTATTKEVAAATNIDR
                     AMLLGAQALANAYGQKAGGHFNMVEKKTDMDNRTEIAISWINGLKKIRFPEKSGKMQD
                     HGVIAVDTAVKL"
     gene            15273..15662
                     /locus_tag="ACHKTW_003676"
     CDS             15273..15662
                     /locus_tag="ACHKTW_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6464616.1"
                     /translation="MAKTILAPSLSERVYTGTHGNESVAEGVFTVNAAEADSVIHLLS
                     LPVGIRINSLQLVSTGGLGTATVSIKSGEHVLIDNSEAVSAKFARYVPVEPYTTQRDG
                     ELVTVTIKTAAATGTLNVLLRYTVVGY"
     gene            15712..16173
                     /locus_tag="ACHKTW_003677"
     CDS             15712..16173
                     /locus_tag="ACHKTW_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6464617.1"
                     /translation="MTEKIAVVYIGPKPVKKDTITGSRTLFPRLEPVHVDSAMAWQLL
                     GFPDVWVRHEELDDVLKKQQQNEQLRQAQQAQERVLAARAEAENSFVVSVNGQEVDLS
                     KLTSARLATLCEAEELDIHKDPKETAEAFRIRVREAFRRRVAETEQHGGTE"
     gene            16157..16720
                     /locus_tag="ACHKTW_003678"
     CDS             16157..16720
                     /locus_tag="ACHKTW_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6464618.1"
                     /translation="MAELSDFLPYVRRHISGPLNIMMTDALSMAAVAFSRQSLVCRRE
                     VTVVPVAGKEIVLPYDKDDEECVHIIRISDDNHELFVGRDVDISSGRSLRFACSPGEV
                     SVLYAVAPKAGRSQIPDELLTWPEEVAAGALERLFMQTGVSWSDPLRAQYFSVQFSEG
                     IRRAYRHTLATSPYSSYRNPVRRQRFY"
     gene            16720..17370
                     /locus_tag="ACHKTW_003679"
     CDS             16720..17370
                     /locus_tag="ACHKTW_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6682 family protein"
                     /protein_id="HFO6464619.1"
                     /translation="MTTITEIIGRVNTQLVDPMMVRWPLQELCDYYNDAVRAVILARP
                     DAGASLETISCVPGARQVLPDGVIQLLDVICLSDGSAVRPLSREVLDAQYPEWPTMKG
                     IPECFISNDLSPRVFWLFPVPDKEISIDAVVSRIPEAVYVLTQDDDTPVPLEEAYVNP
                     LVDWMLFRAFSKDAAGGAESGLAAQHYQSFVEQLGIKQGADSALSARKKVFNGGGV"
     gene            17367..>18173
                     /locus_tag="ACHKTW_003680"
     CDS             17367..>18173
                     /locus_tag="ACHKTW_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HFO6464620.1"
                     /translation="MSVVVSGTLKSPDGEAISGANITLTALTVSPDALSGTSASAVTR
                     EGGYYGMTMDPGEYAVSVTVKGKTAVYGRVRIEGTESTVTLNMLLRRSLVEVSIPGEL
                     LTDFRQIQNNVADDLATIRRLNEDTATKNTQATQSKESAAASAKSASDSAKTATSRAA
                     EAGQKATDATEAATRAITAAGNAEESSTRAGESEKAAGADAEKARQHAEKARLAQESA
                     GEILKRAEAATVSAEEARRMAENARGPRGPQGETGPKGDVGPKGETGPVGP"
BASE COUNT         4684 a         4124 c         5130 g         4235 t
ORIGIN      
        1 acagcatttt gctctacgag tttgccagcc cccccagtgg ctggcttttt tatgtccgta
       61 acatcctgtg tatcaataaa tgttgttgtc tacgtacgtc aagtagtcgc atgagatctg
      121 accagatatg ttaaggttgc agctctcttt gaatataatt atcattttca ttacgttatt
      181 gttacgttta tccggtgcgc cgtaaaacgc cgtccttcag ggggtggagg atgtcaagaa
      241 tatagttatc gtatggtgct caaggagtat tgtgtaatat gaaaataatt atttttagag
      301 tgctaacttt tttctttgtt atcttttcag ttaatgtggt tgcgaaggaa tttaccttag
      361 acttctcgac tgcaaagacg tatgtagatt cgctgaatgt cattcgctct gcaataggta
      421 ctccattaca gactatttca tcaggaggta cgtctttact gatgattgat agtggcacag
      481 gggataattt gtttgcagtt gatgtcagag ggatagatcc agaggaaggg cggtttaata
      541 atctacggct tattgttgaa cgaaataatt tatatgtgac aggatttgtt aacaggacaa
      601 ataatgtttt ttatcgcttt gctgattttt cacatgttac ctttccaggt acaacagcgg
      661 ttacattgtc tggtgacagt agctatacca cgttacagcg tgttgcaggg atcagtcgta
      721 cggggatgca gataaatcgc cattcgttga ctacttctta tctggattta atgtcgcata
      781 gtggaacctc actgacgcag tctgtggcaa gagcgatgtt acggtttgtt actgtgacag
      841 ctgaagcttt acgttttcgg caaatacaga ggggatttcg tacaacactg gatgatctca
      901 gtgggcgttc ttatgtaatg actgctgaag atgttgatct tacattgaac tggggaaggt
      961 tgagtagtgt cctgcctgac tatcatggac aagactctgt tcgtgtagga agaatttctt
     1021 ttggaagcat taatgcaatt ctgggaagcg tggcattaat actgaattgt catcatcatg
     1081 catcgcgagt tgccagaatg gcatctgatg agtttccttc tatgtgtccg gcagatggaa
     1141 gagtccgtgg gattacgcac aataaaatat tgtgggattc atccactctg ggggcaattc
     1201 tgatgcgcag aactattagc agttgagggg gtaaaatgaa aaaaacatta ttaatagctg
     1261 catcgctttc atttttttca gcaagtgcgc tggcgacgcc tgattgtgta actggaaagg
     1321 tggagtatac aaaatataat gatgacgata cctttacagt taaagtgggt gataaagaat
     1381 tatttaccaa cagatggaat cttcagtctc ttcttctcag tgcgcaaatt acggggatga
     1441 ctgtaaccat taaaactaat gcctgtcata atggaggggg attcagcgaa gttatttttc
     1501 gttgactcag aatagctcag tgaaaatagc aggcggagat tcataaatgt taaatacatc
     1561 tcaattcagt cagttgttgc cggtctgata atagatgtgt tagaaaattt ctgcatggtg
     1621 aatccccctg tgcggagggg cgactggtga acggtatgat ctctttgatg atcgtaagcg
     1681 agaatacgcg ggtttggtgg caccagaccg aactcaccgg gaggcacccg gcaccatgca
     1741 gtattcagag attaggcata tatccaggct cctcatcgca ggagcctttt tacatgcaaa
     1801 aaaaaaccgc tcctgggaag agcggctggc aagaagaaac aatatgaaca attaattaac
     1861 gatgggaata ataccttaga gtaatcacct tgcgcaactg taagggcata tttcatcttt
     1921 gcgggctgtt tttctgtgtg gcttctgtgt ttccggaggg cagcctgtac cttttctgac
     1981 tcagaacatt atcccggccg ggaggattca tggcatttaa acactacgat gtggtcaggg
     2041 cggcatcgcc gtcagacctt gctgaacgaa taactcaaaa actgaaggaa gggtggcagc
     2101 cttatggtag tgcgctgatt tcgacagctg gttatggtgc agagtttatc cagccagttg
     2161 tgagtgaggg ggagcttccc tcacttgcag agtcaggcaa tcacccgcat gtttcagcga
     2221 agcctgaagc agcgccggaa tattactatg tgattgcgct tgccggtcag tccaatagca
     2281 tgtcatacgg tgaaggtctt ccgttgccgg agacatatga ccgtccggac ccgcgtatta
     2341 agcagctggc gcgccgcagt acggtgacac cgggcggtgc agcatgcaaa tataacgaca
     2401 tcattccggc ggaccattgt ctgcatgatg tgcaggacat gagccgtctt aaccatccga
     2461 aagcggacct gtcaaagggg cagtacggaa ccgtggggca ggggctgcat atcgccaaaa
     2521 aactgctgcc gtttataccg gcgaatgcgg gtattctgct ggttccgtgc tgtcgtggtg
     2581 gttcagcgtt caccaccgga gctgatggca catacagtga cgctggcggt gcctcggaga
     2641 attcaacccg ctggggtgtg gacaagccgc tgtataagga ccttatcggt cgaacaaaag
     2701 cagcactgaa gaagaacccg aaaaatgtgc tgtttaccgt ggtgtggatg cagggggaat
     2761 ttgattttga cggcacgccc ggaaatcacg cagcacagtt tggtgcgctg gttgataaat
     2821 tccgtgcaga cctgacggat atggcaggtc agtgcgttgg tggctctgct ggcggtgttc
     2881 cctggatatg cggggacacg acgtatttct ggaagcagaa gaacgaatcc acgtaccaga
     2941 cggtgtacgg cagctacaaa aacaaaacgg aaaagaatat tcatttcgta ccgttcatga
     3001 cggatgagaa cggggtgaat gtgccgacga acaaaccgga agaagacccg gacattccgg
     3061 gtatcggata ttacggttcg aaatggcgtg acagctcagc cacctggacg tcacaggaca
     3121 gggcgagcca tttcagttca tgggcacgcc gcgggattat ttccgaccgt ctggcaacgg
     3181 cgattctgcg ccatgcggga agagtggcgc taaacgcggg ggcatcatcg acagtatcag
     3241 aggtgcgccc gtcatcgcct tccggtgcag aagccacagg cgtcacaaca ctgctctctt
     3301 accttgccag cgagtcagag ggaagcctga aagtacaggg atggtcagcc agtggcggca
     3361 gggcagaagt ggtcagcgat gcggagggaa ccggaggtaa ggctgtgaag gtgaccaagg
     3421 aagccggtaa aagcacctgg gtgctggagt acgctgcggg caacggcgcg gctctgttac
     3481 agaaaggggg acagattcgc tgccgcttta aggcttcggg agcgctggcg gcgaaccagt
     3541 atgttatggc gttttactgg ccggtatcgt cactgccaca gggcgttgtc ctgaccggcg
     3601 atggggggaa taacctgctg gcagcgttct acatccagac ggatgcaaaa gacctgaatg
     3661 tgatgtacca caatgcgaaa gtggcgacaa acaacctgaa actgggaagc tttggcgcat
     3721 ttgataacga atggcatacg ctggctttcc gctttgccgg gaataacagc cttcaggtga
     3781 cgccggttat tgatggtcag gatggcacac cgttcacgct gacgcagtca ccggtcagtg
     3841 cattttcggc ggataagttg catgtgacag acattaccaa aagtgcgact tacccggtac
     3901 tgatagacag cattgcggtg gaagtgaata acgcggacgc cgctgcatga taaaaaaagc
     3961 cgccagttac cggaatggac tggcggtggt aatacctatg gagaaaaaat aaaggaacga
     4021 tactttcgta ctctgttttt ttaaatgaaa acagttctta ttgtcaacaa taacggaaag
     4081 aaattatgac atttctgaac cagttaatgc tgtacttctg tacggtggtc tgtgtgctgt
     4141 atctcctttc gggtggatac cgggccatgc gtgacttttg gcgcagacag attgacaaaa
     4201 gggccgctga gaaaatcagc gccagtcagt cagccggaag caaacccgaa gagccgctcg
     4261 tttagcggca actttcttaa tcacaccttt cgacgagaaa atcccatgtc agaaattaca
     4321 tccctggtca ctgctgaagc agtgaaggac gtcctgcgct ctgaagaagt cctgagtgca
     4381 ctgaaacaga aacttcgcca taacctggaa gcgcgtcttg atgcagaagt ggatgccatt
     4441 ctggatgaac tgctgggcgc accggcagct ccggagccgg aaggcatcgc gggagagggg
     4501 agtgcttcag atagcggtga ccccacacct gacagcgaca tgatgatgta agcatgcgtc
     4561 agggaccatc ggtgtgtgcc ggtggtcttt tttattgttg tgagcttccg gattgcggga
     4621 ggcggggtat gtaccagatg gaaaaaatca caacaggttt gtcatacacc acgtcagcgg
     4681 tgggaacggg ctactggttc ctgcagttgc tggacagggt ttccccgtct cagtgggcgg
     4741 caataggcgt gctggggagt ctgctgtttg gtctgctgac gtacctgacg aacctgtatt
     4801 tcaaaatcag agaggaccgg cgtaaggcgg cgcggggaga gtaagctgat gagccggaaa
     4861 ttccgctatg gtttatcagc tgtcgttctg gcgctgattg ccgcaggggc ttctgcgcct
     4921 gaaatcctgg atcagtttct ggatgaaaaa gaaggtaacc acaccacggc ataccgtgat
     4981 agtgcgggta tctggaccat ctgccgtgga gccacccggg tggatggtaa gcctgttatt
     5041 cctggcatga agctgtcgaa ggaaaaatgc gaccgggtta acgccattga gcgtgataag
     5101 gcgctggcat gggtggagaa aaacatcaaa gtgccactga ctgaacccca gaaagcgggg
     5161 atcgcgtcat tctgcccgta taacattggc cccggtaagt gtttcccgtc gacgttttat
     5221 aaacgaatta atgcaggcga tcgccggggt gcatgcgagg cgattcgctg gtggattaaa
     5281 gacggtggca gagactgccg tatccgctca aataactgtt atggtcaggt atcccgtcgc
     5341 gaccaggaga gtgcgctggc gtgctgggga atcgacagat aagcagaata ttttgctgaa
     5401 aaatgacgtt ggccaacgcg ggcggataac acgaaatcct gcgaactggc aaaacctaag
     5461 tgaataaagt caggaacatt gtttcacgct gaggcaccgt aatggtgtct ttgtcatttc
     5521 tgcgcttcgc acaagcgtaa ataaaccaaa gaacctttca ggatgagccc tggtggataa
     5581 ccggctggtc tggttaaccc tctttgggct ggttattcct gtgcgcaggg ttcatcacta
     5641 aaaggaaata accgatgaat atgatgaccg tgccgtttca cggcgattct ctttatgtgg
     5701 ttaaccataa tggcgaaccg tatgttccaa tgaaacccat tgttgagggg atggggctgg
     5761 cttgggctcc acaatttact aagttaaaac aacgatttgc caaaggcgtt acggaaatca
     5821 taataccttc tgctggtggt gagcaaacaa tgacttgtct tgctctgcgt aagctggctg
     5881 cctggcttca gaccatcaac tcaaacaaag ttaaacctga aattcgcgac aaggtaatcc
     5941 agtatcagga agagtgcgac gatgttctct atgaatactg gacgaagggg gttgtcgtta
     6001 atccccgtcg aatgagtgtg atggaagaac tcaatcaggc ttgcgctgac atgaaacggg
     6061 ataaaaacat tgccagtgtg tttgctaccg ggctgaatga gtggaaacag gttaaagccg
     6121 cgcatgtatc aaaaatccgc acattgataa acgaagcgaa tctgctgatt gattttgtcc
     6181 tagctgatac aggcaaaggg aaaataacaa aggcggattg atggagtagt ggctaatgat
     6241 atcggataaa ctcataacgc tggcgaagat cctctgtgta atcgtcggca tttcattttt
     6301 agtcatgctg gttgccattt tcttttccac cgcctggcga gtcctgacgt tatcgggact
     6361 ggtggggtga aagagagatg aaccgtgttc tgtgtgtggt gattattgtc ctggcggttg
     6421 gctgtggtgc gctgtggctg gcaacaaacc attaccgtga caacgcgctc acctacaaag
     6481 cgcagcgaga taaaaaagtc agagagctgg agctggcaaa cgcaaccatt actgatatgc
     6541 aggtgcgcca gcgtgatgtt gctgcgcttg atgctaaata ctcgagggaa ttagccgatg
     6601 cgagagctga aaatgaaact ctgcgtgctg atgttgccgc tggtcgtaag cgcctgcggc
     6661 tcaacgccac ctgccccggt accgtgcgtg aagccaccgg cacctccggc gtgggcaatg
     6721 atgccagcgt cgaactctct ccagttgctg gacgaaacgt tctcggtatc agagacggaa
     6781 tcatcagcga ccaggcagca ctgagaacgc ttcaggaata tatccgcact cagtgcttga
     6841 attaacagag ccagcatgat cgctggcttt ttcatatctg aatttcaccg cgcaccgcag
     6901 cgcatgataa ccaccgaacc tgcccctttg gaatgagcct ttgaggatac cagttagtgc
     6961 tggcgagcct cggtgggctg gtttcctatg cggcaaaggt tcatttcaaa gaacaggtat
     7021 acgacatgaa atcattaacc ctcttcaatc agccaatccg tgtcgctgaa gatggcatga
     7081 tctgcctcac tgatatgtgg aaagccagtg gtaaaagtga atctgaatct ccataccact
     7141 acctgagaaa caagcagacc aaagagttct tagccgagct ggagaaaaac cacgaatctg
     7201 tggtttttac tgagcgcggt gtacacggtg gaacatatgg cgggaagttt gttgcttatg
     7261 attatgcagc atggctaaac cccggattta aatatgcagc ctataaagtc ctggatgact
     7321 acttcaccgg agaacttcag catcgcaaca gcttaagtgc gcagctcaat atgaagtgcc
     7381 atgagtttga tcagaaaaaa gatatggcga gcttctgtgg acaagggctc gcggcatggc
     7441 gctatacgaa gccagtgttg gtcgctgaga ttaactcctt ggctaaccag ctgcagatta
     7501 cgatccccgg gcttccggga tgagtgatcg tgtcattgaa tgcgcctcca gagcggggcg
     7561 cgacttctca gagttcatga aaggcgagaa gggcatgatg gaagcattgg cctcggtgga
     7621 tgagtttggc gagcagctgc gcctcaacgg ctgtgtcaat catcactttg tcagctacat
     7681 gatgcggaac tcgatcatgc aggcattcat ggacatggca aaagccgaga ggaaagaaga
     7741 gcgccggcgt aagcgagcgg aagcaaaagc gaagtagtta ttacaaaagc cattccctac
     7801 agagtggctt tgataatggc ttatacccta cacgggataa cttaactgat atccctttta
     7861 acggataaag gcatttcagc ctgacatagc catgcgccgc atcgtcgccg tattcccgca
     7921 ttaacagaga ccgcagccac cttatctgcg tgagtgtgcg gggataatca aaaacgatgc
     7981 acaccgggtt ttctcatttt tcacgagatg ggagcgattt cccgcgaagc cgcctgtccg
     8041 gtgcggtggt ggaagaaacc ggataaaaca accgcattgt gcaaatatcg atcaaatatg
     8101 gtgctgctgt gtgaaatctg aaaaatcaca gcggtcatta tgcatcagtt tttaacacag
     8161 gacgtcagaa cgtgacatgg caaagctgga ctggaaaaag ctggagcagg cattccgacg
     8221 cgaacatgcc gaaacgggaa taacattact ggactggtgc cggaagaaaa agattaatta
     8281 caacaccgcc agaacccgta taaaaatggg caaaatcgat catgaaattg atcataaaac
     8341 cgatcatgaa atcgatcatg acatctcaga tgaagaaccc tgcaatgacg cgggttccgg
     8401 cgatgaaaaa tgcgcaaaaa actctgaaaa aaactgcgca aattcggcag aaacgaaacg
     8461 gattcgtggt tcccgacttt taccaccttc aaacgctttt tctcagcgaa acacccacgc
     8521 tgtaagacac cgtggatacg cgaagtatct tgaggcagat aacctcatgg atgatgcgtc
     8581 cgatatggtg ctgttcgatg aactggtgtt cacccgggcc cgcgcacttt cagtaactaa
     8641 ggcacttaaa gggatgttcg ccgacctgga agaggcaact gacgtggaaa cccgtgttgc
     8701 tctttacgac aaaatactca aagctgaaca ggcccttgac cggaatattg cccgtatcga
     8761 gtcaattgaa cgctcattgc tgacgctgga cgtcctggct gagacagcac caaaacttcg
     8821 tgctgaccgg gaaagaatca acgccgccag agataaactc agagctgaaa ccgatattct
     8881 gaccagccag cgtcggggcg ttgttacgcc tgtcagtgac atcgtgtcat cgctgcatga
     8941 aatgagtaat tcggggagac tggatgacat tccggaagaa tgaaccgcga tgtgatgagc
     9001 cgtcagaaat gaccgaggct gaacaacgtc tgttcatcat gactaaactg agcaatccct
     9061 ggtggcggct caatcatctc tacaaaatac agaacgaaaa aggtgaactg gtcaccttca
     9121 gaatgcgacc ggcgcagcgc cagttgttcc ggagcatgca caataaaaat attatcctga
     9181 aagcgcgcca gctgggattt tccacagcca ttgatattta tcttctcgac caggcattat
     9241 tcattccgca tctcaaatgc gggatcgtcg ctcaggataa acaggctgcc agtgaaattt
     9301 tccgcacaaa aattgctgta ccgtttgatc atctccctga ctggctgaga gcctcattca
     9361 ccatcgttga acgtcgtagc ggtgccagcg gtggctatat cctgtttggt cacggctcga
     9421 gtatccaggt ggcaacctca ttccgttcag gtacggtgca gcgcctgcat atctcagagc
     9481 acggcaaaat ttgcgcgaaa tatccggcta aggcgaaaga actgcgaacc ggtacgctta
     9541 atgccgtctc tgatgaatgc attatttttg atgagtccac agctgaaggc gtgggtggtg
     9601 atttttacga gatgagtaac cgagcacagg agatcacagc atcaggctta tcgttgacgg
     9661 cacaggatta taaattccat ttttacgcct ggtggcagga tcctaaatac agcgcccgag
     9721 tgccggaaag cgggctgaag ctgtcacggg aaaaaatgac gtatttttct gcggttgaga
     9781 aggcaatgaa catcacgctt actgatgaac agaagcagtg gtacatcaat aaggaaactg
     9841 aacagcgtga ggaaatgaag caggagtttc cctcaacgcc acaggaggcg tttctgacgt
     9901 ccggacgacg tgtgttcagt gccgaaagta cgttgcaggc agaatcattc tgttcgccac
     9961 cgctgattgt ttatgacatt gaacctgtta caggaaggaa gactaaagcg cagtctctgc
    10021 gtgacgggaa taaagccgaa cagcaccgga cgctgatgaa ttatctgctg gtctgggagc
    10081 taccggatcc agatgaagag tatgtctgcg gagcggatat tgctgagggg cttgagcacg
    10141 gggaccgctc atcgctggat atcatcagat gcagtaatgg tgagcaggtg gcccactggt
    10201 ttggtcatct tgatgcggag ctttttgctc atctcattgc gcaggtctgt cgtatgtaca
    10261 acaacgcgtt tgtggggccg gagcgtaaca atcacggaca tgccgttatt ctgaaactca
    10321 gggagctcta tccgacgcgt tatatctaca acgaacagca ccttgatcag gcatatgatg
    10381 acgatacgcc ccgccttggc tggctgacaa cccgtcagag caaacctgtt ctgaccgaag
    10441 gaatgaaaac gctcctgaat aatggaatat cagggatccg ctggtcaggc acattatcgg
    10501 aaatgaacac ctacgtttat gacgcgaaag gctccatgaa tgcacaggaa ggctgttttg
    10561 atgatcaact catgagctac atgattgccc aggagatgcg cgccagaatg cctgtgaggg
    10621 taaaacagaa aacggataaa cgcagaacta cacactggat ggctcactga tgaaaaatga
    10681 aactaacacc atggcgacga aaaacgacaa tggagccacg ccgcgttttt ctcagcgcca
    10741 gttacaggcg ctttgttctg atattgacag ccagcctaaa tggcgtgatg ccgcaaacaa
    10801 ggcctgtgcg tattacgatg gcgatcagtt gccaccggaa gttcttcagg ttctgaaaga
    10861 tcgcggtcag ccgatgacta tccataacct catcgcgcct accgtcgatg gcgttctggg
    10921 aatggaggcc aaaacacgga ctgatctggt ggtgatgtca gacgagccag atgatgaaac
    10981 tgaaaaactg gctgaagcta ttaatgctga atttgccgat gcatgccgcc ttggcaatat
    11041 gaataaagcc cgctctgatg cctatgcgga acaaatcaag gcgggcctca gttgggtgga
    11101 ggtcaggcgc aacagcgatc cgttcggacc tgaattcagg gtatccacag tcagcagaaa
    11161 tgaggttttc tgggactggc tcagccggga atccgacctg agtgactgcc ggtggctgat
    11221 gcgtcgccgc tggatggata ccgatgaggc aaaagccacg ttcccgggaa tggctcaggt
    11281 tatcgattat gctattgatg actggcgtgg ttttgtcgat accacgatta ctgaaggcca
    11341 gcccagtccg ttgatgagtg catgggaaga gtatcagtca tgggatcgca gggagaatga
    11401 atggcttcag cgtgaacgcc gtcgtgtgct gcttcaggtg gtttattacc gtacattcga
    11461 gcgtcttccg gtgattgaac tcagtaatgg acgggtggtg gcttttgata aaaataatct
    11521 gatgcaggcg gtggctgtgg catccgggcg ggttcaggtg aaagtcgggc gggtaagtcg
    11581 tattcgtgaa gcctggtttg tgggcccaca ctttattgtg gatcgcccct gtagtgcacc
    11641 gcaggggatg tttccgctgg ttcctttctg gggataccga aaggataaaa ccggggagcc
    11701 atacgggtta atttcccgcg ccattccggc acaggatgag gtgaattttc gtcgtatcaa
    11761 gctgacctgg ctgcttcagg ccaaacgcgt gattatggac gaggatgcca cccagttgtc
    11821 agacagcgac ctgatggagc agatcgaacg tccggatggc attattaaac tgaatccggc
    11881 ccgaaaaaat cagaaaagtg tcgcagatgt ttttcgggtt gagcaggatt ttcaggttgc
    11941 cagccagcag tttcaggtca tgcaggaatc ggaaaaactt atccaggata ccatgggagt
    12001 gtattctgca tttctcgggc aggattcagg tgcgacgtca ggcgtggcta tcagtaacct
    12061 ggtggagcag ggggccacaa cccttgcgga aatcaacgat aactaccagt ttgcctgcca
    12121 gcaggtggga agactgttgc tggcttatct tctcgatgac ctgaaaaaac gccgtaatca
    12181 tgcagtggtg attaatcgcg atgatcgcca gcgtcgccag accattgtcc tcaatgctga
    12241 aggtgataat ggtgaactga ccaatgatat ttcaaggtta aatacacata ttgcgctggc
    12301 gcctgttcag cagacaccgg cgtttaaggc acagcttgca cagagaatgt cagaggttat
    12361 tcaggggctg ccgcctcagg tgcaggctgt tgtgctcgac ctgtgggtta atcttctgga
    12421 tgtgccgcag aaacaggagt ttgttgagcg tattcgtgct gcgctgggga cgccaaaatc
    12481 accggatgaa atgacgccgg aagaacagga agtagcggca caacaacagg cacttcagca
    12541 acaacaggca gaactccaga tgcgcgagat ggctggcaga gtggcaaaac tggaagctga
    12601 cgccgccagg gcacatgcag ctgcacaacg ggataatgcc agtgcacagc gggaagtcgc
    12661 cctgacacag gggcagcgtt atgtggatgc gcttaaccag gcacatacgg cagaaatcat
    12721 taccggcgta cagaatatgg aacaggagca ggacgttctt cagcaacaga tgctgtatac
    12781 gttacaacag cggatgaatg aaatgtcgct ctgaaaactc tggcttcaac tgaaccccgt
    12841 catcgtacgg ggttttttgt ttccggaggt aagcgttccg ggagcggtgc gcttattcgc
    12901 gggggcagcg ataagcctta tttactcaac cattcggatc tgtccgataa acagaccatg
    12961 cggagttatt tatggatttt gaatttacgg gtgaagaaac cccggaacaa ctggaaaaaa
    13021 tgctggaagg acttggggat gtggatattg acagtcacgc acaggacgtc gtgacggaag
    13081 ataccacgga aaaacatgcg gatgaggaag cacagactca gacgggcgat aacaatgtgg
    13141 caccgacgcc ggatgccagt gtggagcaga cgcaggacgt gaaggagccg gaagcgaagg
    13201 gggtgctcac ccgcgacggt aaacacgtca ttccctatga agtccttgag gctgaacgtt
    13261 ccggtaagca acgggccgaa caggaagccg cacttcttcg tgggcagata gctgaagaaa
    13321 aacgcagggt ggaactgctg acgtctcaga tccaccaggc cggtatgaag cccacaccgt
    13381 taccggaaaa cgaaaaaatt tctgatgagc agattgcccg tatcagggag atgtatccgg
    13441 aaattggtga cgcggtggct tcgctcatcc gtaaaaataa ctatctccag tcccgtgttc
    13501 agcaatcaac acagcaggca gaaggtaatg gtggtgagga tttatcaccg gttcttgatg
    13561 cgatgaatgc cgtgccggtg ctgaaaacgt ggcaggagtc cgatccggat cgcttctcgg
    13621 ttgctgtatc catcgacggg aagctccaga atgaccccgc atggaaagac aaaacgctca
    13681 ctgaacgttt cgctgaagtg gcccgtcgta cgcaggttgc tttcggtgaa gtcagtgagt
    13741 cgtctgctga caaccaggca gacaaaacgg atatccggaa aacggcggaa gagaaagtga
    13801 agaccgctga acgggagcag gcagtaccag cttccccgtc agatttaggc accacggcct
    13861 ccgtcggaac cggtgataat tttgaacggt tacttggcgc ttcgcattca gaggcagagg
    13921 cgattatgcg cggtatgacg aatgctgaaa tagacgcact tctggagaag ctcgggtaac
    13981 ttactgaagg agtactgaag taatgacgac tgtaacatca gcccaggcga ataagctgta
    14041 tcaggtggcg ctttttactg ctgccaaccg caaccgctcg atggttaata tccttactga
    14101 acagcaggaa gcgccaaaag cggtttcgcc ggacaagaaa agcacgaagc agaccagcgc
    14161 aggtgcgccg gttgtccgta tcacagacct taacaaacag gccggtgatg aagtgacctt
    14221 cagcatcatg cacaaactct caaaacgtcc gacgatggga gatgagcgtg ttgaaggtcg
    14281 tggtgaggat ctcagccatg ctgacttctc cctgaaaatc aatcagggac gtcacctggt
    14341 ggatgcaggc ggacgtatga gtcagcagcg cacgaagttt aacctggcat cctctgccag
    14401 aacgcttctg gggacgtact ttaatgacct gcaggaccag tgtgcgatag tgcatcttgc
    14461 gggagctcgt ggtgattttg ttgctgacga caccattctg ccgacagcgg agcaccctga
    14521 attcaaaaaa atcatgatca acgatgtact gcctccgaca catgaccgtc acttttttgg
    14581 cggtgatgcg acaagctttg agcagattga agcggcagat attttttcta ttggcctggt
    14641 ggacaatctc tccctgttca ttgacgaaat ggcgcatccg ttacagccgg ttcgtctgtc
    14701 cggtgatgaa cttcacggag aagatccata ttacgtcctg tacgtcacgc cgcgtcagtg
    14761 gaatgactgg tacacctcga cgtccggtaa ggactggaac cagatgatgg ttcgtgccgt
    14821 gaaccgtgca aaaggtttta atcatccgct gttcaaaggt gaatgtgcga tgtggcgcaa
    14881 tatcctggtt cgtaagtatg cgggtatgcc gatccgtttc tatcaggggt caaaggttct
    14941 ggtatcagag aataacctga cggcaaccac gaaagaggtc gctgctgcaa ccaatattga
    15001 ccgcgccatg ttactggggg ctcaggcgct ggcaaatgct tacggtcaga aggcgggcgg
    15061 tcacttcaac atggttgaga agaaaacgga tatggataac cgtactgaga tagcaataag
    15121 ctggatcaac ggtctgaaaa aaatccgttt ccccgagaag agcggcaaga tgcaggatca
    15181 cggcgtgatt gccgttgata cagcagtgaa gctctgattt tttcctttcc ccatgccggg
    15241 ttttcgcccg gctttttcag gagtcattaa ttatggcaaa gactatcctt gccccgtcac
    15301 tgagtgaacg ggtctatacg ggtacgcacg gtaatgagtc ggtggcagaa ggcgtattta
    15361 cggtgaatgc tgcggaagcg gacagtgtta ttcatcttct ctcactgcca gtgggcatcc
    15421 gtatcaactc actccagctg gtttcaacgg gtggtctggg tactgcaacc gtcagcatta
    15481 agtccggtga gcatgttctc atcgataaca gcgaagctgt ttctgcaaaa tttgccagat
    15541 atgtgccagt ggagccgtac accacacagc gtgacgggga gctggttact gtcaccatta
    15601 agactgccgc tgcaaccggc accctgaatg ttctgctgcg ttataccgtg gtgggatact
    15661 gattaaaacc ttccggcccg cgtcatgcgg gctttttatc cggggaatta tatgactgag
    15721 aaaattgccg ttgtctatat cggcccaaaa cccgtgaaaa aggacaccat taccggaagt
    15781 cgcacgctgt tcccacgtct tgagccggtg catgttgaca gtgcgatggc ctggcaactg
    15841 ctggggtttc cggatgtctg ggttcgtcat gaagagcttg atgatgttct gaaaaagcaa
    15901 caacagaatg agcagttgcg gcaggcacag caggcgcagg aaagagtgct tgctgcgcgg
    15961 gcagaagcgg agaacagttt tgttgtttct gttaacgggc aggaggtgga tttaagtaag
    16021 ctcacctcag cacggctggc gacgctgtgt gaggcagaag agctggatat tcacaaagac
    16081 ccgaaagaaa cggctgaggc attccgtatc cgggtgcgtg aggcatttcg ccgtcgtgtt
    16141 gcggagactg aacagcatgg cggaactgag tgatttttta ccgtatgtcc gtcgtcatat
    16201 cagcggtcca ctgaacatta tgatgacgga tgctctgtca atggctgctg tggcattcag
    16261 ccgccagtcg ttggtgtgcc gtcgggaggt tactgttgta ccagtagcag gaaaagaaat
    16321 cgtgcttccg tatgacaaag atgatgagga gtgcgttcat atcatccgta tctctgacga
    16381 taatcatgag ctttttgtcg gtcgggatgt ggatatcagc tccggacgct ccctgcgatt
    16441 tgcctgttct cccggtgagg tgagcgtgct ttatgccgtc gctccgaaag ccggacgcag
    16501 ccagataccg gatgaactcc tcacatggcc tgaagaagtg gctgcggggg cacttgagcg
    16561 gttgttcatg cagactggtg tttcatggtc agatccgtta cgcgctcagt atttttctgt
    16621 gcagttttct gaggggatcc gtcgggcata tcgtcataca ctggcgacaa gcccgtactc
    16681 ttcataccgc aaccctgtac gcaggcagag gttttactga tgacgacgat tacagaaatc
    16741 atcggacgtg tgaatacaca actggttgac ccgatgatgg ttcgctggcc cctgcaggaa
    16801 ttgtgcgatt attacaatga tgctgtgagg gcagtgattc tggcgagacc ggatgctggc
    16861 gcaagcctgg aaacaatcag ttgtgttcct ggcgcccgtc aggttttgcc cgatggtgta
    16921 atacaacttc ttgacgtgat atgcctcagt gacggtagtg cagtcagacc attatcccgg
    16981 gaggtgctgg atgcgcagta tcctgagtgg cccacaatga agggcattcc tgaatgtttt
    17041 atcagcaacg acctgtcccc gcgcgtattc tggctgtttc ctgtccctga caaagagata
    17101 agtattgatg cagtggtaag ccggataccg gaggcagtgt atgttctgac gcaggacgat
    17161 gatacgccag ttccactgga agaggcttat gttaacccac tggtggactg gatgttgttt
    17221 cgtgctttca gtaaggatgc tgccggtggt gcagaatcgg ggctggctgc gcagcattat
    17281 cagagttttg ttgagcaact tgggatcaaa cagggggcag acagtgcatt gtctgcccgt
    17341 aaaaaagtgt ttaacggagg tggagtgtga gtgttgttgt ttcggggacg ctgaaatctc
    17401 ctgatggtga ggcgatatca ggagcaaata ttaccctgac ggcgctgaca gtttcaccgg
    17461 atgcgctcag cggcaccagt gcgtcggcag tgacccgtga aggtggatat tacggaatga
    17521 cgatggatcc gggggagtat gcggtttcgg tgacggtgaa agggaagact gctgtctacg
    17581 gacgtgtgcg tattgagggg accgaaagta cggtgacgct caatatgctg ttacgccgca
    17641 gtcttgttga ggtgagcata cccggagagc tgctgacaga tttccggcag atacagaata
    17701 atgtggctga tgaccttgct actattcgtc gcctgaatga agacacggcg acaaaaaaca
    17761 ctcaggccac acagtcaaaa gaaagtgcag cagccagtgc gaagagtgca tctgacagtg
    17821 caaagacggc aaccagcagg gcggctgaag ccggacaaaa agcgactgat gccactgagg
    17881 ccgcgacccg tgcaatcaca gcagcgggga atgcagagga aagctcgacc cgtgccggag
    17941 agtctgaaaa agccgccgga gctgatgcag aaaaagccag acagcatgct gaaaaggcca
    18001 ggctggcgca ggagagcgcc ggagagatcc ttaagcgggc agaggctgcc actgtcagtg
    18061 ctgaagaggc cagacgtatg gctgagaatg cacgggggcc ccgggggcct cagggagaaa
    18121 ctggtccgaa gggggatgtc ggtcctaaag gcgaaacagg tccagtgggc cct
//
LOCUS       DAZFQD010000109        18135 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.109, whole genome shotgun sequence.
ACCESSION   DAZFQD010000109 DAZFQD010000000
VERSION     DAZFQD010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18135
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.109"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            261..986
                     /locus_tag="ACHKTW_003681"
     CDS             261..986
                     /locus_tag="ACHKTW_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO6464621.1"
                     /translation="MLPGCCKNGIVISKIPVMQAGLKEVMRTHFPEYEIISSASAEDL
                     TLLQLRRSGLVIADLAGESEDPRSVCEHYYSLISQYREIHWVFMVSRSWYSQAVELLM
                     CPTATLLSDVEPIENLVKTVRSGNTHAERISAMLTSPAMNETHDFSYRSVILTLSERK
                     VLRLLGKGWGINQIASLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQGARELN
                     LPSVYGDAAEWNTAELRREMSHS"
     gene            944..1621
                     /gene="bglJ"
                     /locus_tag="ACHKTW_003682"
     CDS             944..1621
                     /gene="bglJ"
                     /locus_tag="ACHKTW_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HFO6464622.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIADDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAADILLCMRHCEA
                     SNVLHPY"
     gene            complement(1659..2447)
                     /gene="fhuF"
                     /locus_tag="ACHKTW_003683"
     CDS             complement(1659..2447)
                     /gene="fhuF"
                     /locus_tag="ACHKTW_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HFO6464623.1"
                     /translation="MAYRSAPLYEDIIWRTHLQPQDAGLAQAVRATIAKHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRVACFWVDVCEDKNATPHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTTGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGDCTLK"
     gene            2588..2824
                     /locus_tag="ACHKTW_003684"
     CDS             2588..2824
                     /locus_tag="ACHKTW_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HFO6464624.1"
                     /translation="MLQRMLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLVLTP
                     VMLYHKQLRHYVLLPSCLALIAGIMLMIMNLNQG"
     gene            complement(2863..2949)
                     /locus_tag="ACHKTW_003685"
     tRNA            complement(2863..2949)
                     /locus_tag="ACHKTW_003685"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2913..2915),aa:Leu,seq:cag)
     gene            complement(3217..4248)
                     /gene="rsmC"
                     /locus_tag="ACHKTW_003686"
     CDS             complement(3217..4248)
                     /gene="rsmC"
                     /locus_tag="ACHKTW_003686"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HFO6464625.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSRQMGDNARFSLVATVDDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANGVEGEVFASNVFSEVKGRFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
     gene            4351..4764
                     /gene="holD"
                     /locus_tag="ACHKTW_003687"
     CDS             4351..4764
                     /gene="holD"
                     /locus_tag="ACHKTW_003687"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HFO6464626.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIAIPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            4733..5179
                     /gene="rimI"
                     /locus_tag="ACHKTW_003688"
     CDS             4733..5179
                     /gene="rimI"
                     /locus_tag="ACHKTW_003688"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006474 - N-terminal protein amino acid
                     acetylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFO6464627.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRVLLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            5194..5871
                     /gene="yjjG"
                     /locus_tag="ACHKTW_003689"
     CDS             5194..5871
                     /gene="yjjG"
                     /locus_tag="ACHKTW_003689"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HFO6464628.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLKVEAGLLNDAFINAMAEICTPLPGAVS
                     LLNAIRGNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHHREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            5962..7551
                     /gene="prfC"
                     /locus_tag="ACHKTW_003690"
     CDS             5962..7551
                     /gene="prfC"
                     /locus_tag="ACHKTW_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFO6464629.1"
                     /translation="MTLSPYLQEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLDGLVEWA
                     PAPMPRQTDTRTVQASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTLGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     SRLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            7944..8549
                     /gene="osmY"
                     /locus_tag="ACHKTW_003691"
     CDS             7944..8549
                     /gene="osmY"
                     /locus_tag="ACHKTW_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HFO6464630.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRHVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            8676..8837
                     /locus_tag="ACHKTW_003692"
     CDS             8676..8837
                     /locus_tag="ACHKTW_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HFO6464631.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            8959..10032
                     /locus_tag="ACHKTW_003693"
     CDS             8959..10032
                     /locus_tag="ACHKTW_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HFO6464632.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRIFEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            10029..10811
                     /locus_tag="ACHKTW_003694"
     CDS             10029..10811
                     /locus_tag="ACHKTW_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFO6464633.1"
                     /translation="MICRFIDTHCHFDFPPFSGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAEKYQPLYAALGLHPGMLEKHSDVSLDQLQQALERRPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKRHDLPCTGVVHGFS
                     GSLQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAVRVFDVLCELRPEPEDEIAEVLLNNTYALFSVSG"
     gene            complement(11126..11989)
                     /locus_tag="ACHKTW_003695"
     CDS             complement(11126..11989)
                     /locus_tag="ACHKTW_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HFO6464634.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVLWNAVVCEQCDTCLKMCPQHATPMAQSMSVDEV
                     LSHVRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQHLTGRDNQQIKRSICLLAERGKLAELRLLVIPDQ
                     VDYLHHIDELATFIKRLGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRG
                     VSRLIFPALYL"
     gene            complement(11961..13511)
                     /locus_tag="ACHKTW_003696"
     CDS             complement(11961..13511)
                     /locus_tag="ACHKTW_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HFO6464635.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQDEIDVRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAELKQVSPNLTFIYDPEITPDDLLLEVAKNICECSKPHIAN
                     GPVHDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYC
                     QQQIAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCEKEG
                     IAARYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGISSDIGTTPGAR
                     LPYGDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMRE
                     FTANVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISH
                     EQQMRFSQ"
     gene            13769..14548
                     /gene="deoC"
                     /locus_tag="ACHKTW_003697"
     CDS             13769..14548
                     /gene="deoC"
                     /locus_tag="ACHKTW_003697"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFO6464636.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGNEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            14675..15997
                     /gene="deoA"
                     /locus_tag="ACHKTW_003698"
     CDS             14675..15997
                     /gene="deoA"
                     /locus_tag="ACHKTW_003698"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009032 - thymidine phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HFO6464637.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDIRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVIHAKDENSWQDAAKAVKAAIKLADKAPESTPTVYRRISE"
     gene            16049..17272
                     /gene="deoB"
                     /locus_tag="ACHKTW_003699"
     CDS             16049..17272
                     /gene="deoB"
                     /locus_tag="ACHKTW_003699"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFO6464638.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            17352..18071
                     /gene="deoD"
                     /locus_tag="ACHKTW_003700"
     CDS             17352..18071
                     /gene="deoD"
                     /locus_tag="ACHKTW_003700"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFO6464639.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGVDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
BASE COUNT         4371 a         4593 c         4867 g         4304 t
ORIGIN      
        1 tgatttctgt ttttcattaa gtattcctaa caacttaatg tagatatttt aaatgtctcc
       61 aggctatttc accaggttat catttgatgc taaccttctg acacatgcag tggagttgtt
      121 gtgcagcagg agtatgctga tatgaaagtg aatgctaagg ataatttatt cgcttaatct
      181 attaatttgc tggaaacatt taaggaatga atatcctgaa taaaccatca tgatttttag
      241 acagtggatg tggaggaaat atgttgccag gatgctgcaa aaatggaatt gttatcagta
      301 aaatacctgt tatgcaagca gggttaaaag aggtcatgag gactcacttc cctgaatatg
      361 aaataatatc cagcgcctct gcggaggacc ttaccttatt acaattacgt cgttccggat
      421 tagtcattgc tgatttagcc ggtgaaagtg aagatcctcg ttctgtttgt gaacattatt
      481 attctttaat ctcacaatat cgggaaattc actgggtttt catggtgtca cgctcctggt
      541 attcccaggc agtagaattg ctcatgtgcc ctacggcgac gttattgtct gatgttgaac
      601 ccattgagaa tctggtcaag accgtacgtt ccggcaatac gcacgcagag cgtatcagcg
      661 ccatgctgac ctctccggca atgaatgaaa ctcatgattt tagctatcgc tccgtcattc
      721 tcactctttc agagcgcaag gtactgcggc tattaggtaa aggatggggc atcaaccaga
      781 tagcttcgtt gcttaagaaa agtaataaaa ctatcagcgc ccaaaaaaac agtgcgatgc
      841 gtcgactggc aattcacagc aacgctgaaa tgtatgcatg gataaatagc gcgcagggtg
      901 caagagaact taacttgcct tctgtttatg gagatgccgc agaatggaac acagccgaat
      961 taagaagaga aatgtcgcac tcatagaaaa atgcgtcatg agcagtatcg gtattgagag
     1021 tttattcaga aagtttgcgg gtaaccctta taagctccat acctatacca gtcaggagtc
     1081 atttcaggat gccatgtcgc ggatctcatt tgcggcggtc attttttctt tttctgccat
     1141 gagaagtgag cgcagagagg gattatcttg cctgactgaa ctggcgatta agtttccgcg
     1201 tacccggcgt ttagttattg cggatgatga tattgaagct cggctgattg gttcattgtc
     1261 gccatcaccg ctggacggtg tattaagtaa agcgtcaacg ctggagattt ttcatcagga
     1321 actctttttg tcattaaatg gtgtacgtca ggcgaccgac cgactgaaca atcagtggta
     1381 cattaaccaa agccggacgc taagcccgac ggagagagaa atattgcgct ttatgtcgcg
     1441 tggctactca atgacacaaa ttgccgagca gcttaaacgc aatatcaaaa cgatccgtgc
     1501 acataaattt aatgtgatgt cgaaactggg cgtcagttct gatgcagggt tgttggaggc
     1561 cgcagatatt ctgttatgta tgcggcattg cgaagcaagt aatgtgttgc atccctatta
     1621 atccgcatga tgccgggttt acttccccgg cagtgctttc atttcagcgt acaatcgcca
     1681 cattgctgca catccggtaa gcgataacgc tggcagcagg tccggcgcac cagcaggccg
     1741 tcgcgtagca ccacggtacg ccagagtgga ttatcttcac cggtagtaag cgttttctca
     1801 aaaaagaggg catggcgcag cgattcaacc gtcgcctcgc cgagcagttg cttcatctca
     1861 gtgagatacc agttgatcaa ataaccggta ttactccaga taagtttgcc gttgatctct
     1921 ccggtcgctt ctagtgcctg cacaaccgga accagcgcct ggctgattaa cgtttccatt
     1981 cgctgctgcg gcgaatgtgg tgttgcgttt ttatcttcac acacatcgac ccagaaacag
     2041 gcgacgcgtc cggtttcgtg aaactcagca tggaaatgtt ccggcgacac atctaatgcc
     2101 ttttcctgcg tcagtagcgc cagcattaat ggtggcacca tcaggccgat ataccattgt
     2161 gcccatagtg agatcagcgg tttgttctcg cggatcatcg ttggttggtt gcgatagata
     2221 tgatcggaat agaccgcgag tagagaactt agcgcattcg gtgatgacca ttgcgccagc
     2281 gtcatggcgt taagcggagc gggttcatcc aggcggataa actcgagcaa atgttcacga
     2341 tgtttggcga tcgtcgcacg tacggcttgt gcaagcccag catcctgcgg ctggagatgc
     2401 gttcgccaga tgatatcttc atagagcggt gcggaacgat aggccataat cgggatagta
     2461 atctaaatga taatgattgc taatcctagc gataggttta cccgatagca agggatttct
     2521 ctggcttgca aatgataaaa attatcatat gatattggtt atcattatca atgaaagaga
     2581 tgaaaccatg ttgcaacgta tgctgggcag tggctgggga gtgttgctgc caggattgct
     2641 gattgcaggg ctgatgtatg cggatttatc gccggatcag tggcggattg tcattctgat
     2701 gggattagta ttgacgccgg tgatgctgta tcacaaacag ttacggcatt acgttttgct
     2761 accatcgtgc ctggcgctta ttgctggcat catgctgatg ataatgaatt tgaatcaggg
     2821 atgaaaaatc aaggaagaaa caagaaagga agtaaagata attggtgcga ggggggggac
     2881 ttgaaccccc acgtccgtaa ggacactaac acctgaagct agcgcgtcta ccaattccgc
     2941 caccttcgca taccatcaat tcttaaaaag aattgctacc acggaggcgc attctagtgg
     3001 ttttcagctt ttcgtcaata gttaattatc gccggaggtg taattgctgg aaaaatgtcc
     3061 atcaggaaac tagcgtgcag gtttggtagg catgcggggg cagatgccag atgcgacgct
     3121 ggcgcgtctt atctggccta cgaagggcta acgtgcaggt tttgtaggtc ggataaggcg
     3181 ttcacgccgc atccgacacg gtattcggcg agataattaa cctttcttcg cctggcgggt
     3241 cataatggcg cgatacacct tgaaacgccc ggtttgcgca atcacttcat ggaagccaaa
     3301 tgtctcatcc agcacgtccg ggtaaggcag gaaggcgttc gctacaattc gcagctcgcc
     3361 gccgctatta agatgacgca ccgcgccgcg aatcagcgtt tgcgccgcat ccaggctggt
     3421 ttgcatccca tcgtggaacg gcgggttgga gatgatcata tcaaaacgac ctttcacctc
     3481 ggaaaagacg ttgctggcaa agacttcacc ttcaacaccg ttggccgcaa gtgttgcgcg
     3541 gctggcttct accgctggcg cagagacatc gcacaaggtg agacgaattt tcggcgaatg
     3601 gcgcgcaaag gcaactgaaa gcacacccgc gccacagccg acatccagca ctttaccttt
     3661 cgtgtgcggg gttaacgtcg agagcagcaa ctggctaccg acatccagac cgtcgcggct
     3721 aaacacgcca ggcagcgttt tgaccgtcag gccatcgacg ctgtattcgc cccagaattt
     3781 atcggcatca aataccggct gtttttccag acgaccaaaa tagaggccac agcgacgagc
     3841 gctgtcgact ttattcaacg gcgcataatc tgccagcatc tgctcggcgc tgcgcacgcc
     3901 gctgcggttc tcgccaacga caaaaatatc tgtccccact ggcagcagag aaagtaaatt
     3961 catcaactgg aactgggctt ccggtttgtt cttcggccag tagtaaatca gcgtatcgca
     4021 atctgcgacg tcatccaccg tggcgaccag actgaaacgg gcgttatccc ccatctggcg
     4081 gcttaatacc tgccagtggt ggaattgctg ggtatgagca cggctggccg cggtatctaa
     4141 acgcgcgggc aggtcatcct gtaagtctcc ggcaaacaga atacggcttt gttcgaaatc
     4201 atcactgtga cgcagcaaga cttcacttgc cggggtaaat gcagacatgg aatgctcctc
     4261 aattgatact ggcggcgatt atagccatat gttggcgcgg tatcgacgaa tttgctatat
     4321 ttgcgcccct gacaacagga gcgattcgct atgacatccc gacgagactg gcagttacag
     4381 caactgggca tcacccagtg gtcgctgcgt cgccctggcg cgttgcaggg ggagattgcc
     4441 attgcgatcc cggcacacgt ccgtctggtg atggtggcaa acgatcttcc cgccctgacc
     4501 gatcctttag tgagcgatgt tctgcgcgca ttaaccgtca gccccgatca agtgctgcaa
     4561 ctgacgccag aaaaaatcgc gatgctgccg caaggcagtc gctgcaacag ctggcggttg
     4621 ggtactgacg aaccgctatc actggaaggc gctcaggtgg catcaccggc gctcaccgaa
     4681 ttacgggcaa acccaacggc acgcgccgcg ttatggcaac agatttgcac atatgaacac
     4741 gatttcttcc ctcgaaacga ctgatttacc agcggcttac cacattgaac aacgcgccca
     4801 cgcctttccg tggagtgaaa aaacctttgc cagcaaccag ggcgagcgtt atctcaactt
     4861 tcagttaacg caaaacggca aaatggcggc gtttgcgatt acgcaagtgg tgctggatga
     4921 agctacattg ttcaatattg cggtcgatcc tgactatcag cgtcagggat tgggaagggt
     4981 gctgctggaa catctgatcg acgaactgga aaaacgcggc gtggcgacac tatggctgga
     5041 agtccgtgct tcaaacgctg ccgccattgc cctgtacgaa agtttaggct ttaacgaggc
     5101 gacgattcgc cgcaattact accccaccac ggacggtcgc gaagacgcca tcatcatggc
     5161 gttgccaatc agtatgtaag acaaggtgga ataatgaagt gggactggat tttctttgat
     5221 gccgatgaaa cgctgtttac ctttgactca ttcaccggcc tgcagcggat gtttcttgat
     5281 tacagcgtca cctttaccgc tgaagatttt caggactatc aggccgttaa caagccactg
     5341 tgggtggatt atcaaaacgg cgcgatcact tcattacagc ttcagcacgg gcggtttgag
     5401 agctgggccg aacggctgaa agttgaagca ggcttgctta acgatgcctt tattaatgcg
     5461 atggcggaaa tctgtacgcc gttgccgggc gcggtttctc tgcttaacgc cattcgtggc
     5521 aacgctaaaa tcggcatcat caccaacggt tttagcgcct tgcagcaagt gcgtctggaa
     5581 cggacgggcc tgcgtgatta cttcgatttg ctggtgattt ccgaagaagt tggcgttgct
     5641 aaaccgaata agaaaatttt cgattatgcg ctggaacagg cgggcaatcc tgaccgttca
     5701 cgcgtgctga tggttggcga cactgccgag tccgatattc tcggtggcat caacgccggg
     5761 cttgcgacct gctggctgaa tgcacaccat cgcgagcaac cagaaggcat cgcgcccacc
     5821 tggaccgttt cttcgttgca tgaactggag cagctcctgt gtaaacactg attgcctccc
     5881 ccccgttgat gggtaaaata gccgcaattt ttcgttttca acaagcgcgg cgcgatgccg
     5941 cttactcaag aagaaagaat tatgacgttg tctccttatt tgcaagaggt ggcgaagcgc
     6001 cgcacttttg ccattatttc tcacccggac gccggtaaga ctaccatcac cgagaaggtg
     6061 ctgctgttcg gacaggccat tcagaccgcc ggtacagtaa aaggccgtgg ttccaaccag
     6121 cacgctaagt cggactggat ggagatggaa aagcagcgtg ggatctccat tactacgtct
     6181 gtgatgcagt ttccgtatca cgattgcctg gttaacctgc tcgacacccc ggggcatgaa
     6241 gacttctcgg aagataccta tcgtaccctg acggcggtgg actgctgcct gatggttatc
     6301 gacgccgcaa aaggtgttga agatcgtacc cgtaagctga tggaagttac ccgtctgcgc
     6361 gacacgccga tcctcacctt tatgaacaaa cttgaccgtg atatccgcga cccgatggaa
     6421 ttgctcgatg aagttgagaa cgagctgaaa attggctgcg caccgatcac ctggccaatt
     6481 ggctgcggca agctgtttaa aggcgtttac cacctttata aagatgaaac ctatctctat
     6541 cagagcggta aagggcacac cattcaggaa gtccgcattg ttaaagggct gaataacccg
     6601 gatctcgatg ctgcggttgg tgaagatctg gcacagcagc tgcgtgacga actggaactg
     6661 gttaaaggcg cgtctaacga gttcgacaaa gagctgttcc ttgcgggcga aatcactccg
     6721 gtattcttcg gtactgcgct gggtaacttc ggcgttgatc atatgttgga tggcctggtg
     6781 gagtgggcac ctgcgccgat gccgcgtcag actgataccc gtaccgtaca agcgagcgaa
     6841 gacaaattta ccggcttcgt atttaaaatt caggccaaca tggacccgaa acaccgcgac
     6901 cgcgtggcgt ttatgcgcgt ggtatccggt aaatatgaaa aaggcatgaa gctgcgccag
     6961 gtgcgcaccg cgaaagacgt ggtgatctct gacgcgctga cctttatggc gggtgaccgt
     7021 tcgcacgttg aagaagcgta tcctggcgat atcctcggcc tgcacaacca cggcaccatt
     7081 cagatcggcg acacctttac cctgggtgag atgatgaagt tcaccggtat tccgaacttc
     7141 gcaccagaac tgttccgtcg tatccgcctg aaagatccgc tgaagcaaaa acagctgctc
     7201 aaagggctgg tacagctttc cgaagagggc gcggtgcagg tgttccgtcc gatctccaac
     7261 aacgacctga tcgttggtgc ggttggtgtg ctgcagtttg atgtagtggt atcgcgcctg
     7321 aagagcgaat acaacgttga agcagtatat gaatcagtca acgttgccac cgcccggtgg
     7381 gtagaatgtg cggacgcgaa gaaatttgaa gagttcaagc gtaagaacga aagccaactg
     7441 gcgcttgatg gcggcgataa tctcgcttac atcgctacca gcatggttaa cctgcgcctg
     7501 gcacaggaac gttatccgga cgttcagttc caccagaccc gcgagcatta attcttgcct
     7561 ccagggcgcg gcaaccgcct cgccctgtca atttcccttc cttattagcc gcttacggaa
     7621 tgttcttaaa acattcactt ttgcttatgt tttcgctgat atcccgagcg gtttcaaaat
     7681 tgtgatctat atttaacaaa gtaatgacat ttctgacggc gttaaatacc gttcaatgcg
     7741 tagatatcag tatctaaagc cgtcgattgt cattctaccg atattaataa ctgattcaga
     7801 ggctgtaatg gtcgttattc atcacccatc gcttttgtga tggcgaccat tgacttctgt
     7861 agagggtgaa gtctctccct attcagcaat gcaacctcgt gttgccaggc tcaaattacg
     7921 agcaaacata caggaataaa tcgatgacta tgacaagact gaagatttcg aaaactctgc
     7981 tggctgtaat gttgacctct gccgtcgcga ccggctctgc ctacgcggaa aacaacgcac
     8041 agactaccaa tgaaagcgca gggcaaaaag tcgatagctc tatgaataaa gtcggtaatt
     8101 tcatggatga cagcgccatc accgcgaaag tgaaggcggc cctggtggat catgacaaca
     8161 tcaagagcac cgatatctct gtaaaaaccg atcaaaaagt cgtgaccctg agcggtttcg
     8221 ttgaaagcca ggcccaggcc gaagaggcag tgaaagtggc gaaaggcgtt gaaggggtga
     8281 cctctgtcag cgacaaactg cacgttcgcg acgctaaaga aggctctgtg aagggctacg
     8341 cgggtgacac cgccaccacc agtgaaatca aagccaaact gctggcggac gatatcgtcc
     8401 cttcccgtca tgtgaaagtt gaaaccaccg acggcgtggt tcagctctcc ggtaccgtcg
     8461 attcccaggc acagagcgac cgtgctgaaa gtatcgccaa agcggtagat ggtgtgaaaa
     8521 gcgttaaaaa tgatctgaaa actaagtaat tcgtcgtaat tcgtcctccc gaactttgtc
     8581 gggaggcgcg atgtgcacca cactaaaaat atcgcattga gtgattttca cgctcatatc
     8641 aagcggtgac attaactatg gtaaaggaga cgcttatgtt tcgttggggc atcatatttc
     8701 tggttatcgc gttaatcgcc gccgcacttg ggtttggtgg tctggccggt accgctgcag
     8761 gcgcagctaa aattgtcttt gtcgtcggga ttattctgtt cctggtgagt ttgttcatgg
     8821 gccgaaaacg cccctagatt tcagaccatt aagaaaattt tatccaaagc cagtccagcg
     8881 gactggcttt tgcggtttta gcgaataata aattgcgtta ctttgtcatt ctttcacaac
     8941 aaaaacagga aggcagaggt ggggcagcga atacctgtaa cgcttggtaa tattgcgccg
     9001 ttgtcgctaa ggccgttcca gcctggacga atagctctgg tgtgcgaagg cggcggacag
     9061 cgtggaattt tcacggctgg cgtgctggat gagtttatgc gcgcgcagtt taatcctttc
     9121 gatctttatc tcggcacatc tgccggggcg cagaacctct cggcgtttat ctgcaatcag
     9181 cccggttacg cgcgcaaagt catcatgcgc tataccacaa aacgcgaatt tttcgatcca
     9241 ttgcgctttg tccgtggagg aaatcttatc gatctcgact ggctggtgga ggccaccgca
     9301 agccagatgc cgttgcaaat ggataccgcc gcgcggttgt ttgacagcgg caaatcgttt
     9361 tatatgtgcg cctgtcgtca ggatgactac gcgcctaatt actttttacc aaccaaacaa
     9421 aactggctgg atgtgattcg cgcctccagt gcgatacctg gcttttatcg tagcggagtg
     9481 tcgctggaag gcattaacta cttggatggc gggatcagtg atgcgattcc ggttaaagag
     9541 gcggcaaggc agggcgctaa aacgttggtc gtcatccgca ctgtgccgtc acaaatgtac
     9601 tacacgccgc agtggttcaa acgcatggaa cgctggctgg gtgacagtag cctgcagccg
     9661 ctggtcaatc tggtgcagca tcatgaaacc agctatcgtg acattcagca atttattgag
     9721 aaaccaccgg gcaagctgcg gatattcgaa atttatccac cgaaaccatt acatagtatc
     9781 gcgcttggca gtcggattcc ggcgctgcgt gaagactata aacttgggcg tttatgcggt
     9841 cgttatttcc tcgccacggt tggcaagcta ttaactgaaa aagcgccgct tacccgccat
     9901 ctggtgccag tggtgacgcc ggaatcgatc gttattccgc ctgcgccagt cgccaacgat
     9961 acgctggttg ccgaagtgag cgatgctccg caggcgaacg acccgacatt taacaatgag
    10021 gatctggctt gatttgccgt tttatcgata cccactgcca ttttgatttc ccgccgttta
    10081 gtggcgatga agaggccagc ctgcaacgcg cggcacaagc gggcgtaggc aagatcattg
    10141 ttccggcgac agaggcggaa aattttgccc gcgtgcaggc gttagcggaa aaatatcaac
    10201 cgctgtatgc cgcattgggg ttgcatccgg ggatgttgga aaaacatagc gatgtgtctc
    10261 ttgatcagct acagcaggcg ctggaaaggc gtccggcgaa ggtggtggcg gtgggggaga
    10321 tcggtctgga tctcttcggc gacgatccgc aatttgagag gcagcagtgg ttactcgacg
    10381 aacagctgaa actggcgaaa cgctacgatc tgccggtgat ccttcattcc cggcgtacac
    10441 acgacaaact ggcgatgcat cttaaacgtc acgatttacc gtgcactggc gtggttcacg
    10501 gtttttccgg cagcctgcaa caggccgagc ggtttgtaca gctgggctac aaaatcggcg
    10561 taggcggtac tatcacctat ccacgcgcca gtaaaacccg cgatgtcatc gcaaaattac
    10621 cgctggcatc gttgttgctg gaaaccgacg cgccggatat gccgctcaac ggctttcagg
    10681 ggcagcctaa ccgaccggaa caggctgtgc gggtgtttga cgtgctttgc gagttgcgcc
    10741 cggaaccaga agatgagatt gccgaagtgt tgcttaataa tacgtatgcg ttgtttagcg
    10801 ttagtgggta gtttaccctt cgtaggcagc atccggcagt cggcgcataa tgcctgatgc
    10861 gacgcttaac gcgtcttatc atgcctacag gtttgtgccg aaccgtaggc cggataaggc
    10921 gttcacgccg catccggcag ttggcgcaca atgcctgatg cgacgcttga cgcgtcttat
    10981 catgcctaca ggtttgtgcc gaaccgtagg ccggataagg cgttcacgcc gcatccggca
    11041 gtcggcgcat aatgcctgat gcgacgcttg tcgcgtctta tcatgcctac aagtctgtgc
    11101 cgaaccgtag gccgtatccg gcatgtcaca aatagagcgc cggaaatatc aaccggctca
    11161 ccccgcgcac ctttaacgca tcagccaacg gctcaacgtc ttccggcgtg gcgctcgccc
    11221 agctttgcgc ctcgccatac acgccgtggg catgaaacgc attcaggcga accggaacat
    11281 cgccaagtct cttgataaac gtcgccagct catcgatgtg atgcaaataa tccacctggt
    11341 ctggaattac cagcaaacgc agttccgcca gcttgccgcg ctctgcaagc aaacagatgc
    11401 tgcgcttaat ctgctgatta tcgcgtccgg ttaagtgttg atgacattcg ctcccccacg
    11461 ctttgagatc gagcattgcg ccgtcacaca ccgggagtaa tttttcccag ccggtttcgc
    11521 tcaacatgcc gttactgtcc accaggcagg tgaggtggcg cagttgcgga tcgtttttga
    11581 tagcagtaaa cagcgccact acaaacggca gttgggtcgt ggcttcgccg ccgcttaccg
    11641 ttatcccttc aataaacagc accgctttgc ggacatggct aagcacttcg tccacgctca
    11701 tggattgcgc cattggcgtg gcatgttgcg gacacatctt caggcaggta tcacactgct
    11761 cgcagaccac agcgttccac agcactttgc cgtcaacaat ctgcaacgcc tgatgcggac
    11821 actgtggcac gcactctcca cagtcattgc aacgtcccat cgtccacgga ttgtgacagt
    11881 ttttgcagcg cagattgcag ccctgcaaaa acagagccag acgactgcct ggcccgtcaa
    11941 cgcaggagaa ggggataatc ttactgacta aagcgcatct gctgttcatg gcttatcacg
    12001 cgcggctggc gttccagaat acgagtgttg cgtgcggctt cttcgcccag ccaggtggtg
    12061 ttggtgcgtg aaccttcggc gcgatatttt tctaagtccg acaaacgtac catataaccg
    12121 gtaacgcgaa ccagatcgtt accgctgaca ttggcggtaa attcacgcat tccggcttta
    12181 aaggcaccga ggcaaagctg taccagtgcc tgcgggttac gtttgatggt ttcgtcgagc
    12241 gtcagaatgt cgctgatgcc ggaataataa taagcatgat gcggggcgac agtttgcaga
    12301 tgggtgatcg gatctggctc atcgccatac ggcaaacgcg cgcccggcgt ggtgccaata
    12361 tcggaactga tccccgactg tgcgtgtaac atggcgcgtt tttgccatcc atatttcacg
    12421 ggggtattgg cgacaaactc cgccagttgc gcgctgatgc gataacccac ttcatttgcg
    12481 gcggcttctt taccgtaacg tgcggcaatc ccttctttct cacacagcaa gttaaccgct
    12541 tccgccagcc catacatgcc aaacattggc acaaaacgtt cagggttaat cagcccttct
    12601 ttcaccagga agctattctc aaagaagtgt gattgttgat agaggaattc acaccgcgca
    12661 tcgatgatgg cgatctgctg ctggcagtag tgcggtagag tgcgcgtaaa gaagtcatcc
    12721 agcgattcgc tgcgctcggc aatggctttc aggttaaggc gtaccagcgt gctgccacca
    12781 cccgccagcg gcagtgagtt gtaacagctc acaatcccgt agcccccttt tgtgaaaatt
    12841 ttatcatgca ccggaccgtt ggcgatgtgc ggtttgctac attcacagat gttcttcgcc
    12901 acttccagca gcaggtcatc aggggtgatt tcaggatcgt agataaaggt caggttcggt
    12961 gaaacctgct tcaactctgc gtctgcacgt aagattgcac gggtaatggg cgaatcagac
    13021 gggccgatat tggcgtgcat aaaggcgtct ggcagggttc tgtcgaggta acgccagaaa
    13081 cgttttattc gaacatcgat ctcgtcttgt gttagaattc taacatacgg ttgcaacaac
    13141 gcatccagtt gccccaggta gaccggcatc gatgtgaccg acggtacgtg gtggtaaaga
    13201 atggtcagca gagagagtgc gtcatcaaga tctttcgcgc cttccagctc cagccattcg
    13261 gaaccgttcg ccagaaaacg ggcgtaatcg ggtaagacat agcgcggttt gtacggcgca
    13321 tgaccttcaa acatatcgca gattacaccc tcatccagcg cgcggcgggc ttcggcagga
    13381 agctgcggat aaggcagatt gttttctgct tccagagcca gaaaatggcg cttctgctcc
    13441 gggctaagca ctgggctggt gacaatttgc tggcaacgtt gttgcagtgc attttcatga
    13501 gaagtgggca tcttcttttc cttttatgcc gaaggtgatg cgccattgta agaagtttcg
    13561 tgatgttcac tttgatcctg atgcgtttgc caccactgac gcattcattt gaaagtgaat
    13621 tatttgaacc agatcgcatt acagtgatgc aaacttgtaa gtagatttcc ttaattgtga
    13681 tgtatatcga agtgtgttgc ggagtagatg ttagaatact aacaaactcg caaggtgaat
    13741 tttattggcg acaagccagg agaatgaaat gactgatctg aaagcaagca gcctgcgtgc
    13801 actgaaattg atggacctga ccaccctgaa tgacgacgac accgacgaga aagtaattgc
    13861 tctgtgtcat caggccaaaa ctccggtcgg caataccgcc gctatctgta tctatcctcg
    13921 ctttatcccg attgctcgca aaacgctgaa agagcagggc accccggaaa tccgaatcgc
    13981 tacggtaacc aacttcccac acggtaacga tgacatcgaa atcgcgctgg cagaaacccg
    14041 tgcggcaatc gcctacggtg ctgatgaagt tgacgttgtg ttcccgtacc gcgcgctgat
    14101 ggcgggtaac gagcaggttg gttttgacct ggtgaaagcc tgtaaagagg cttgcgcggc
    14161 agcgaatgta ctgctgaaag tgatcatcga aaccggcgaa ctgaaagacg aagcgctgat
    14221 ccgtaaagcg tctgaaatct ccatcaaagc gggtgcggac ttcatcaaaa cctctaccgg
    14281 taaagtggct gtgaacgcga cgccggaaag cgcgcgcatc atgatggaag tgatccgtga
    14341 tatgggcgta gaaaaaaccg ttggtttcaa accagcgggc ggcgtgcgta ctgcggaaga
    14401 tgcgcagaaa tatctcgcca tcgcagatga actgttcggt gctgactggg cagatgcgcg
    14461 tcactaccgc tttggtgctt ccagcctgct ggcaagcctg ttgaaagcgc tgggccacgg
    14521 tgacggtaag agcgccagca gctactaagt aagatgcttt acgcctaatg cgctgcgctt
    14581 atcaggccta cgagacgtat ctacccgtag gccggataag gcgtagacgc atccggcaaa
    14641 agccgcctca tactcttttc ctcgggaggt taccttgttt ctcgcacaag aaattattcg
    14701 taaaaaacgt gatggtcatg cgctaagcga tgaagaaatt cgtttcttta tcaacggtat
    14761 tcgcgacaac actatctccg aagggcagat tgccgccctc gcgatgacca ttttcttcca
    14821 cgatatgaca atgcctgagc gtgtctcgct gaccatggcg atgcgagatt caggaaccgt
    14881 tctcgactgg aaaagcctgc atctgaatgg cccgattgtt gataaacact ccaccggcgg
    14941 cgtcggcgat gtgacttcgc tgatgttggg gccgatggtc gcagcctgcg gcggctatat
    15001 tccgatgatc tccggtcgcg gcctcggtca tactggcggt acgctcgaca aactggaatc
    15061 catccctggc ttcgacattt tcccggatga caaccgtttc cgcgaaatta ttaaagacgt
    15121 cggcgtagcg attatcggcc agaccagctc actggctccg gcggataaac gtttctacgc
    15181 gacccgtgat attaccgcaa ccgtggactc catcccgctg atcactgcct cgatcctggc
    15241 gaagaaactg gcggaaggtc tggatgcgct ggtgatggac gtgaaagtgg gtagcggcgc
    15301 gtttatgccg acctacgaac tctctgaagc ccttgccgaa gcgattgttg gcgtggctaa
    15361 cggcgctggc gtgcgcacca ccgcgctgct caccgacatg aatcaggtac tggcctccag
    15421 tgcaggtaac gcggttgaag ttcgtgaagc ggtgcagttc ctgacgggtg aatatcgtaa
    15481 cccgcgtctg tttgatgtca cgatggcgct gtgcgtggag atgctgatct ccggcaaact
    15541 ggcgaaagat gacgccgaag cgcgcgcgaa attgcaggcg gtgctggaca acggtaaagc
    15601 ggcagaagtc tttggtcgta tggtagcggc acaaaaaggc ccgaccgact tcgttgagaa
    15661 ctacgcgaag tatctgccga cagcgatgct gacgaaagca gtctatgctg ataccgaagg
    15721 ttttgtcagt gaaatggata tccgcgcgct ggggatggca gtggttgcaa tgggcggcgg
    15781 acgccgtcag gcatctgaca ccatcgatta cagcgtcggc tttactgata tggcgcgtct
    15841 gggcgaccag gtagacggac agcgtccgct ggctgttatc cacgcgaaag acgaaaacag
    15901 ctggcaggac gcggcgaaag cggtgaaagc ggcaattaaa cttgccgata aagcaccgga
    15961 aagcacacca actgtctatc gccgtatcag cgaataacgg tatactgatc tgatcattta
    16021 aatttgaagc actgagtacg gagaacatat gaaacgtgca tttattatgg tgctggactc
    16081 attcggcatc ggcgctacag aagatgcaga acgctttggt gacgtcgggg ctgacaccct
    16141 gggtcatatc gcagaagcct gtgccaaagg cgaagctgat aacggtcgta aaggcccgct
    16201 caatctgcca aatctgaccc gtctggggct ggcgaaagca catgaaggtt ctaccggttt
    16261 cattccggca ggaatggacg gcaacgctga agttatcggc gcgtacgcat gggcgcacga
    16321 aatgtcatcc ggtaaagata ccccgtctgg tcactgggaa atcgccggtg tcccggttct
    16381 gtttgagtgg ggatacttct ccgatcacga aaacagcttc ccgcaagagc tgctggataa
    16441 actggttgaa cgcgctaacc tgcctggtta tctcggtaac tgccactctt ccggtacggt
    16501 cattctggat cagctgggcg aagagcacat gaaaaccggc aagccgattt tctatacctc
    16561 cgctgactcc gtgttccaga ttgcctgcca cgaagaaacc tttggcttgg ataaactcta
    16621 cgaactgtgc gaaatcgccc gtgaagagct gaccaacggc ggctacaaca tcggtcgtgt
    16681 tatcgctcgt ccgtttattg gcgacaaagc cggtaacttc cagcgtaccg gtaaccgtca
    16741 cgacctggct gttgagccgc cagcaccgac cgtgctgcag aaactggttg atgaaaaaca
    16801 cggtcaggtg gtttctgtcg gtaaaattgc agacatctac gccaactgcg gtatcaccaa
    16861 gaaagtgaaa gcaactggcc tggacgcgct gtttgacgcc accatcaaag agatgaaaga
    16921 agcgggtgat aacaccatcg tcttcaccaa cttcgttgac ttcgactctt cctggggcca
    16981 ccgtcgcgac gtcgccggtt atgctgcggg tctggagctg ttcgaccgcc gtctgccgga
    17041 gctgatgtct ctgctgcgcg atgacgacat cctgatcctc actgctgacc acggttgtga
    17101 tccgacctgg accggtactg accacacgcg tgaacacatt ccggtactgg tttacggccc
    17161 gaaagtaaaa ccgggctcac tgggtcaccg tgaaaccttc gcggatattg gtcagactct
    17221 ggcaaaatat tttggtactt ctgatatgga atatggcaaa gccatgttct gataatgggt
    17281 gcggcttgat gccctcaccc cggccctctc cctcagggag agggtgaaaa catgttgata
    17341 aggataaaac aatggctacc ccacacatta atgcagaaat gggcgatttc gctgacgtag
    17401 ttttgatgcc aggcgacccg ctgcgtgcga agtatattgc tgaaactttc cttgaagatg
    17461 cccgtgaagt gaacaacgtt cgcggtatgc tgggcttcac cggtacttac aaaggccgca
    17521 aaatttccgt aatgggtcac ggtatgggta tcccgtcctg ctccatctac accaaagaac
    17581 tgatcaccga tttcggcgtg aagaaaatta tccgcgtggg ttcctgtggc gcagttctgc
    17641 cgcacgtaaa actgcgcgac gtcgttatcg gtatgggtgc ctgcaccgat tccaaagtta
    17701 accgcatccg ttttaaagat catgactttg ccgctatcgc tgactttgac atggtgcgta
    17761 acgcggtaga cgcggctaaa gcactgggcg ttgatgctcg cgttggtaac ctgttctccg
    17821 ctgacctgtt ctactctccg gacggcgaaa tgttcgacgt gatggaaaaa tacggcatcc
    17881 tcggcgtgga aatggaagcg gctggtatct acggcgtcgc tgcagaattt ggcgcgaaag
    17941 ccctgaccat ctgcaccgtg tctgaccaca tccgcactca cgagcagacc actgccgctg
    18001 agcgtcagac caccttcaac gacatgatca aaatcgcact ggaatccgtt ctgctgggcg
    18061 ataaagagta attgtgtttc gctgaaaggc gattgtcgtg ttaagccgga gtgggggact
    18121 gctccggctt tttag
//
LOCUS       DAZFQD010000110        17450 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.110, whole genome shotgun sequence.
ACCESSION   DAZFQD010000110 DAZFQD010000000
VERSION     DAZFQD010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17450
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.110"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            93..365
                     /locus_tag="ACHKTW_003701"
     CDS             93..365
                     /locus_tag="ACHKTW_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HFO6464640.1"
                     /translation="MALPRITQKEMTEREQRELKTLLDRARIAHGRVLTNSETNSIKK
                     EYIDKLMVEREAEAKKARQLKKKQAYKPDPEASFSWSANTSTRGRR"
     gene            complement(366..1238)
                     /gene="rluF"
                     /locus_tag="ACHKTW_003702"
     CDS             complement(366..1238)
                     /gene="rluF"
                     /locus_tag="ACHKTW_003702"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HFO6464641.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDVVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKVKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            1450..2139
                     /gene="pepE"
                     /locus_tag="ACHKTW_003703"
     CDS             1450..2139
                     /gene="pepE"
                     /locus_tag="ACHKTW_003703"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HFO6464642.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTAKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(2230..3861)
                     /locus_tag="ACHKTW_003704"
     CDS             complement(2230..3861)
                     /locus_tag="ACHKTW_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HFO6464643.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(4081..7764)
                     /gene="metH"
                     /locus_tag="ACHKTW_003705"
     CDS             complement(4081..7764)
                     /gene="metH"
                     /locus_tag="ACHKTW_003705"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HFO6464644.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDQLV
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAQAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGEDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWDVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKTDDTANAQQAEWRSWEVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRTAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFTIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTRKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            7964..8788
                     /gene="iclR"
                     /locus_tag="ACHKTW_003706"
     CDS             7964..8788
                     /gene="iclR"
                     /locus_tag="ACHKTW_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HFO6464645.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
     gene            9105..10679
                     /locus_tag="ACHKTW_003707"
     CDS             9105..10679
                     /locus_tag="ACHKTW_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
                     /protein_id="HFO6464646.1"
                     /translation="MITRIPRSSFSANINNTAQTNEHQTLSELFYKELEDKFSGKELA
                     TPLLKSFSENCRQNGRHIFSNKDFVIKFSTSVLQADKKEITIINKNENTTLTQTIAPI
                     FEEYLMEILPQRSDTLDKQELNLKSDRKEKEFPRIKLNGQCYFPGRPQNRIVCRHIAA
                     QYINDIYQNVDYKPHQDDYSSAEKFLTHFNKKCKNQTLALVSSRPEGRCVAACGDFGL
                     VMKAYFDKMESNGISVMAAILLVDNHALTVRLRIKNTTEGCTHYVVSVYDPNVTNDKI
                     RIMSESKEDIKHYSLMDFMNVDYSLLKWSNDHVINQSVAIIPALPKEQLLMLKGSVDE
                     ITPPLSPATMNLLMAIGQNHQLTQLMIQLQKMPELHRTEMLTAYNSGHMNVINTIFNA
                     LPTLFNTFKFDKKNMKPLLLANNSNEYPGLFSAIQHKQQNVVETVYLALSNHARLFGF
                     TAEDIMDFWQHKAPQKYSAFELAFELGHRVIAELILNTLNKMAESFGFTDNPRYIAEK
                     NYMEALLKKASPHTVR"
     gene            complement(10648..12384)
                     /gene="aceK"
                     /locus_tag="ACHKTW_003708"
     CDS             complement(10648..12384)
                     /gene="aceK"
                     /locus_tag="ACHKTW_003708"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008772 - [isocitrate dehydrogenase
                     (NADP+)] kinase activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HFO6464647.1"
                     /translation="MPRGLELLIAQTILQGFDAQYGRFLEVTSGAQQRFEQADWHAVQ
                     QAMKNRIHLYDHHVGLVVEQLRCITNGQSTDAAFLLRVKEHYTRLLPDYPRFEIAESF
                     FNSVYCRLFDHRSLTPERLFIFSSQPERRFRTIPRPLAKDFHPDHGWESLLMRVISDL
                     PLRLRWQNKSRDIHYIIRHLTETLGTDNLAESHLQVANELFYRNKAAWLVGKLITPSG
                     TLPFLLPIHQTDDGELFIDTCLTTTAEASIVFGFARSYFMVYAPLPAALVEWLREILL
                     GKTTAELYMAIGCQKHAKTESYREYLVYLQGCNEQFIEAPGIRGMVMLVFTLPGFDRV
                     FKVIKDKFAPQKEMSAAHVRACYQLVKEHDRVGRMADTQEFENFVLEKRHISPALMEL
                     LLQEAAEKITDLGEQIVIRHLYIERRMVPLNIWLEQVEGQQLRDAIEEYGNAIRQLAA
                     ANIFPGDMLFKNFGVTRHGRVVFYDYDEICYMTEVNFRDIPPPRYPEDELASEPWYSV
                     SPGDVFPEEFRHWLCADPRIGPLFEEMHADLFRADYWRALQNRIREGHVEDVYAYRRR
                     QRFSVRYGEMLF"
     gene            complement(12665..13969)
                     /gene="aceA"
                     /locus_tag="ACHKTW_003709"
     CDS             complement(12665..13969)
                     /gene="aceA"
                     /locus_tag="ACHKTW_003709"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HFO6464648.1"
                     /translation="MKTRTQQIEELQKEWTQPRWEGITRPYSAEDVVKLRGSVNPECT
                     LAQLGAAKMWRLLHGESKKGYINSLGALTGGQALQQAKAGIEAVYLSGWQVAADANLA
                     ASMYPDQSLYPANSVPAVVERINNTFRRADQIQWSAGIEPGDPRYVDYFLPIVADAEA
                     GFGGVLNAFELMKAMIEAGAAAVHFEDQLASVKKCGHMGGKVLVPTQEAIQKLVAARL
                     AADVTGVPTLLVARTDADAADLITSDCDPYDSEFITGERTSEGFFRTHAGIEQAISRG
                     LAYAPYADLVWCETSTPDLELARRFAQAIHAKYPGKLLAYNCSPSFNWQKNLDDKTIA
                     SFQQQLSDMGYKFQFITLAGIHSMWFNMFDLANAYAQGEGMKHYVEKVQQPEFAAAKD
                     GYTFVSHQQEVGTGYFDKVTTIIQGGTSSVTALTGSTEESQF"
     gene            complement(13999..15600)
                     /gene="aceB"
                     /locus_tag="ACHKTW_003710"
     CDS             complement(13999..15600)
                     /gene="aceB"
                     /locus_tag="ACHKTW_003710"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HFO6464649.1"
                     /translation="MTEQATTTDELAFIRPYGEQEKQILTAEAVEFLTELVTHFTPQR
                     NKLLAARIQQQQDIDNGTLPDFISEMASIRDTDWKIRGIPADLQDRRVEITGPVERKM
                     VINALNANVKVFMADFEDSLAPDWNKVIDGQINLRDAVNGTISYTNEAGKIYQLKPNP
                     AVLICRVRGLHLPEKHVTWRGEAIPGSLFDFALYFFHNYQALLAKGSGPYFYLPKTQS
                     WQEAAWWSEVFSYAEDRFNLPRGTIKATLLIETLPAVFQMDEILHALRDHIVGLNCGR
                     WDYIFSYIKTLKNYPDRVLPDRQAVTMDKPFLNAYSCLLIKTCHKRGAFAMGGMAAFI
                     PSKDEERNNQVLNKVKADKALEANNGHDGTWIAHPGLADTAMAVFNDILGSRKNQLEV
                     MREQDAPITADQLLAPCDGERTEEGMRANIRVAVQYIEAWISGNGCVPIYGLMEDAAT
                     AEISRTSIWQWIHHQKTLSNGKPVTKALFRQMLGEEMKVIASELGEERFSQGRFDDAA
                     RLMEQITTSDELIDFLTLPGYRLLA"
     gene            complement(15869..16798)
                     /gene="metA"
                     /locus_tag="ACHKTW_003711"
     CDS             complement(15869..16798)
                     /gene="metA"
                     /locus_tag="ACHKTW_003711"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008899 - homoserine O-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019281 - L-methionine biosynthetic process
                     from homoserine via O-succinyl-L-homoserine and
                     cystathionine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HFO6464650.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYFRDVEAGLGPEVPYNYFPHNDPQNTPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            16955..17398
                     /locus_tag="ACHKTW_003712"
     CDS             16955..17398
                     /locus_tag="ACHKTW_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFO6464651.1"
                     /translation="MVISIRRSQHEEGEELVAIWCRSVDATHDFLSAEYRAELEELVR
                     SFLPEAPLWVAVNERDQPVGFMLLSGQHMDALFIDPDVRGCGVGRMLVEHALSMAPEL
                     TTNVNEQNEQAVGFYKKVGFKVTGHSEVDDLGKPYPLLNLAYVGE"
BASE COUNT         4282 a         4549 c         4382 g         4237 t
ORIGIN      
        1 aatatttaag aagagaacaa aggaatatta tcagttcagc tcgcaaacga attcccgtac
       61 actgaactct ctggctcact gagggcacca tcatggcact cccccgtatt acccaaaaag
      121 agatgactga acgcgagcag cgtgaactta aaacactgct ggatcgggcg cggattgcgc
      181 atggtcgcgt actgaccaac tccgaaacca acagcattaa gaaagagtac atcgataaat
      241 taatggtcga gcgtgaggct gaagcgaaaa aagcccgcca gttgaagaaa aagcaggctt
      301 ataaaccgga tcctgaagcg tcatttagct ggtcagcaaa tacgtcgacg cgcggcaggc
      361 gctgattagc gccccttctt tttacgcccc ggagaggtaa aacgcttacc gttggacgcc
      421 gggcgaccgc ctttttccgc cgttttttcc atcttaacga ctggacgttt gatgcccgct
      481 gttttcggtt tggccttcac tttaggtttt gcctcggaag aggaattttc gatgagctta
      541 aagaggtcga ttaactcatc atcagttaaa tcgcgccact cacccagcgg aatgccgctt
      601 aaactgacgt tcataatgcg cgtacgttcc agctttttca cttcgtaacc aaagtgctcg
      661 cacatgcgac ggatctgacg gttcagcccc tgcaccaggg taatgcggaa gacaaacggc
      721 gcttcttttt tgactttaca ctttttagtc actgtcccga ggattggcac ccccgccccc
      781 ataccacgaa taaattcatc ggtaatcggt ttatcgaccg taaccagata ctctttctca
      841 tgatcgttac ccgcacggag gatcttattc accagatcgc cgtgattggt gaggaaaatc
      901 agcccctggg agtctttatc caggcggcca atcgggaaca cgcgtttgct gtggttaacg
      961 aaatcgacaa tgttatcgcg ctcgccatct tcggtggtgc tgacaatacc gacgggcttg
     1021 ttcagggcga taagtaccaa atcttcggct tcccgaggct caatcaactg accgtttact
     1081 ttcacaacgt cgccgggttt cacctgatcg ccaatggtgg ctcgcttgcc attaaggaac
     1141 acattgcctt gctcgatata gcgatccgct tcgcggcgtg agcaaattcc gctttcgctg
     1201 atgtatttat ttaaacggac tgatgagtcg ggcagcataa attctcctgt aaaaaacgga
     1261 atataccgta cctttgggtt gataaaaaat agtcatgagc gaactatttg ttatttccat
     1321 tggcttgaat tcgttacctg ctcaaatcac tttccagaag caatgtgcga catcgtgagc
     1381 catgtcagta agtggttaaa ttcgcagtgg tagcatgcct tccgtgattt cttactaacc
     1441 ggagaagtaa tggaactgct tttattgagt aactcgacgc tgccgggtaa agcctggctg
     1501 gaacatgcac tgccgctaat tgctgaacag ttgcagggtc gccgctcagc ggtgtttatc
     1561 cctttcgctg gcgtaacgca gacctgggat gattacacag cgaaaacggc tgcggttctc
     1621 gctccgctgg gtgtttctgt caccggtatt catagcgttg tcgatcccgt tgccgcgatt
     1681 gaaaatgctg agatcgtgat tgtcgggggc gggaatactt tccagttgct gaaacagtgc
     1741 cgcgagcgcg ggctgctggc accaattact gacgtggtta aacgtggtgc gctgtatatt
     1801 ggctggagcg caggcgctaa ccttgcttgc ccaaccattc gtaccaccaa cgatatgccg
     1861 attgtcgatc cgcaaggttt cgatgcgcta aatctgttcc cgctgcaaat caacccgcac
     1921 ttcaccaacg cgctgccgga aggccataaa ggtgaaaccc gtgagcagcg tattcgcgaa
     1981 ctgctggtcg tcgcgccaga actgacgatt attggtctac cggaaggtaa ctggatcaca
     2041 gtgagtaaag gtcacgctac gctgggtggc ccgaacacca cttatgtgtt taaggctggt
     2101 gaagaagcgg tgccgctaga agctggtcac cgtttttaag taaattgccg gatgcatgat
     2161 ccggcatctt gttcatagtg cctgatgcga cgcttgacgc gtcttatcag gcctacagga
     2221 ttcacaatat taatactcgt cccgtccttc atcttcatcc ggctgttcca gcacactgta
     2281 agccaccgaa caaaacagcg agttcaggcg ctgcatatct cccagtaagc ctaaatgtag
     2341 cgaactggtt tcaatgctct gcacgttttg ctgatgcagg cgatcgacgt gggcgtggga
     2401 atagcggcga ttaagaatgc gaaaacgatg tttgctgcga cgcaaacgac gagcgctggt
     2461 gacatcgcca gagaagaaca ccgacattgc cagctttaaa ttactgagca attgctcata
     2521 gagcgcatcc agttccttca acccatcaag cgaaaatgcc cgccgtgctg ccagcgattt
     2581 atcggcaatt tcgctgccca tgcgctcgac gatatcggaa gcctgttcaa ggttgagcga
     2641 catttcaata atctccgccc agcgacgcga ctcttcttct gccagttcct cttttggcat
     2701 ccgcgccaga tacagcttaa tggcggtata gagaacgttg atatcatctg ccagcttacg
     2761 cagctctttc tcctgccgtg gctcgccgtg catcacttta ttcagccctt ccatcatctg
     2821 ttccatggcg tcgccaatgc gcagggtttc gcgcgcggcg ttcgccagag caagcgtggg
     2881 cgtatccagc gcgctgacat ccagatgttt aggccgtagc tgggtatcca gttccggttc
     2941 atcgcgaata atcgttttgc aaaaccgtgc catcgggtca acaaatggca gcatgaccag
     3001 gcaacgtaca aggttgtaga agacgtggaa atagatcacc agttccgctt ttggcaatga
     3061 cagcttcccc attgtctctg ccagcaaatg gacaaacggc aggataatca ggctacccac
     3121 cagcttaaac agcagactac ccagcgcgac acggcgggct gcggcattgg cggcactgtt
     3181 gttgagcatc gccagcaggc cggaaccgag gttagcacca atcaccagac agagcgccac
     3241 ggggaaggag ataatgcctg cggcggtcag agtcgcagtc agcagtacag cagcaaggct
     3301 ggagtagctg ataatggcga acatcgcgcc aatcagcgca tccagcagaa tatcgccggt
     3361 cagcgaggca aagatcacct gaacgccgtt tgcctgggtg atcggcgtta cggcctgcac
     3421 aatcaactcc agcgccagta aaatcagccc aagaccaata ccgacgcggc ccagttgccc
     3481 ggcgcgtgac tgtttgcgtc cgaggaaaaa aatcacgccg ataaaaataa gtaacggtga
     3541 cagccaggat aagtcgaagg tgagaatacg cgccattagc gccgtcccga catctgcccc
     3601 cagcacaatg accagagccg gtgcgagggc taccagatcc tgagcgacaa acgaggtcac
     3661 cagcatggtg gtggcattac tgctctgcac cagtgcggta acgccgatcc ccgcgcaaaa
     3721 ggcgagcggc tttttttcga cgctccggct aaggacagta cgcaaacgcg cgccgaagac
     3781 gcgcattacg ccggttcgaa caatatgagt cccccagacc agcagggcga cggcagaaag
     3841 caggtgaagc agcgttaaca cgagttcagg ttctccttat cgttatatgt tcctgaagca
     3901 attgaatgac caaaggaccg gaaacacgct gactggcatc tttactgtgc cttttgacat
     3961 tcgtttttcc ggctaagatt attccagcct gtttccagta taagtgtttc atattaataa
     4021 agagacaggg cactcattga gcgccctgtt tgttgtaagg aaaagtgaca gatttgtgaa
     4081 tcagtccgcg tcatacccca gattcggtgc cagccagcgc tcaacatcgg agacgctcat
     4141 acctttacgg cgggcatagt cttcaacctg atcgcgctga atttgcgcca ccgcgtagta
     4201 cttgctgtcc gggtggctga aataccaacc cgaaaccgat gcaccgggcc acatggcgaa
     4261 agattctgtg agtttcatgc cggtatgttt ttccacttcc agcagctccc agatggtggc
     4321 tttttccgta tgttccgggc aggccgggta gcccggtgcc ggacggatgc cctggtagtt
     4381 ttcgcggatc agttcttcgt tgctgaggtt ctcgttcggc gcatagcccc agtagacttt
     4441 acgcacacgc tcatggagat actccgcaaa ggcttcggct aaacggtcgg caagcgcttt
     4501 caccatgatt ttgttgtaat catcgtgctg cgcttcaaag gcatcagcca gtgcgtcctc
     4561 ttccagcccg ccagtcacgg caaatgcgcc gatgtaatct gctttaccag aaagcttcgg
     4621 cgcaacgaag tcagcgagac agtagttagc gaatcccgtt ttttcggtct gttgacgcag
     4681 atggtggctg acgttgatca catgggtgcg cgtttcgtca cggtagattt caatgtcatc
     4741 gcccacacgg tttgccggga acaggcccac cacgccacgc ggattcagcg ttttctcggc
     4801 gcttaattta tccagcatgt cgttggcgtc tttaaacagc cgctgcgcct caacgcccac
     4861 cacttcatct tccagaatgc gcggatactt cccggccagc gaccaggtca taaagaacgg
     4921 tgtccagtcg atgtaattac gcagagtttc gatgctggct tcaacttcct gcacgccgag
     4981 acggtgcgcc accggcggcg tgtaagcctg ccagtcaaaa gcgaaatcgt tatcgcgcgc
     5041 cgcttccagc gtgaccggtg gtgtgcgcgg tttcttacgc ccgtgctgaa tacgtacggt
     5101 ttcgtactcc ttgcgggtac gagcgacaaa atcatcacgc tgagtatcgg aaagcagcgc
     5161 cgccaccaca ccaacggtgc gcgatgcgtt ctgtacatac accgtcgggc cgctgtagtt
     5221 ctgctcgatt ttcaccgccg tgtgcgcttt tgaggtcgtc gcgccgccaa tcaatagcgg
     5281 gatggtgaag ccctgacgct ccatctcttt cgccacgtta accatctcgt ccagcgacgg
     5341 cgtgataagc cccgaaagtc caatcagatc agcattcact tctttagcgg tacggagaat
     5401 tttttccgca ggcaccataa cgccgagatc gacaatttcg tagttgttac attgcagcac
     5461 cacaccaacg atatttttac cgatgtcgtg gacgtcgccc ttcacggtgg cgatcaccat
     5521 cttgccgttg gttttgccct gctctttgct ggcttcaata aacggttcga gatacgccac
     5581 cgcctgtttc atgacgcgcg ccgatttgac cacctgtggc aggaacattt tcccttcgcc
     5641 aaacagatcg ccgaccacgt tcatgccgtc catcaacggg ccttcaatca cttcaatcgg
     5701 gcgcgtagcc tgctggcggg cttcttcggt atcctgctcg ataaactcgg taatgccttt
     5761 gaccagcgag tattccagac gtttattcac ttcccacgag cgccactccg cctgctgggc
     5821 gttagcggtg tcgtcggttt tgctgccgcg atatttctcg gcaagctcca gtaaacgctc
     5881 agtgccatca tcgcgacgat taagaatcac atcttccacc gcgtcgcgca gttcagcggg
     5941 caggtcgtcg taaatcgcca gctgcccggc attgacgatc cccatatcca tgccattgcg
     6001 aatagcgtag tagaggaaca ctgcgtgaat ggcttcgcgc accggatcgt tgccacggaa
     6061 cgagaaagaa acgttagata cgccgccgga aattagcgcg tgcggcagtt cgcgtttgat
     6121 gtcttcacac gcgccgataa agtcctgcgc gtagttgttg tgctcttcaa tgccggtggc
     6181 aaccgcgaag atgttcgggt cgaagatgat atcttctggc gggaagccaa cctcttcggt
     6241 gaggattttg tacgcccgac ggcaaatctc gattttccgt gcgcgggtat cggcctgtcc
     6301 ctgttcgtca aaggccatta ccaccactgc cgcaccgtaa cgacgcaaga gcttcgcgtg
     6361 atggataaag gcgtcgacgc cctctttcat cgagatagag ttaacaatgc ctttgccctg
     6421 gatacacttc agaccttttt caatgacatc ccattttgag gagtcgatca taatcggcac
     6481 gcgagcgata tccggttcac cggcaatcag attgagaaaa cgcaccatcg ccgcttcggc
     6541 gtcgagcatc ccttcatcca tgttgatatc gataatctgc gcaccgtttt ccacctgctg
     6601 gcgggccaca tccagtgcct cgctgtattt ctcttctttg atcaggcgct tgaacttagc
     6661 ggaaccggtg acgttggtgc gttcacccac gttcacaaac aggctatctt cgccaatgtt
     6721 cagcggctcc aggccggaca aacggcaggc tacggggatt tccggcagtt tgcgaggcgc
     6781 taatccttct actgcacgac tcatcgctgc aatatgttgt ggcgtggtgc cacagcagcc
     6841 gccgacgata ttgagaaaac ccgcttgcgc ccattcacgt atctgttttg ccatcgtgtc
     6901 ggcgtcgaga tcgtactcac caaaggcgtt gggtagcccg gcgttcgggt gcgcggtgac
     6961 gtagcattcc gcaatccgtg acagctcctg cacgtactgg cgcagttcat cgggccccag
     7021 cgcacagttc aggccaaagg tcagagcttc ggcgtggcgc aatgagttgt aaaatgcttc
     7081 ggtggtctgc ccggagagcg tgcgcccgga ggcgtcggtg atggtgccgg agatcataat
     7141 cggcagctca acgcccagcg cttcaaactc cgttttcacc gcaaaaaccg ccgctttggc
     7201 gttaagggtg tcgaaaacgg tttcaatcag gatcagatcc gcaccacctt ccaccagcgc
     7261 tttggtggat tcgcgatagg ccgccaccag ctggtcaaaa gtgatgttgc ggaatgccgg
     7321 atcgttgacg tccggagaaa tagacgccgt gcggttggtt gggccgagaa caccggcaac
     7381 gtagcgcggt ttctccggcg tgcgcgcggt ccactcgtca gcacaggcgc gcgccagttt
     7441 cgccgccgca aagttgattt ccgccgacag ggattccatc tggtaatccg ccatcgcaat
     7501 ggtcgtggag ttgaaggtgt tggtttcgat gatatccgcg cccgcttcaa agtaggcgtt
     7561 gtggatagcg gcgatcactt ccggtttgct gagcaccagc aggtcgttgt tgcctttgag
     7621 atcgcatggc cagtcggcaa agcgttcacc acgaaaatcg gcttcgttca gtcgatagct
     7681 ctggatcatg gtgcccatac cgccgtccag caccagaata cgttcattta actgcgcacg
     7741 cagttgttcc actttgctgc tcacacttgc tcccgacacg ctcaacccag atttaataaa
     7801 aattcaacaa accatactgg cataaacgca tctgtggtaa aagcgaccac cacgcaacat
     7861 gagatttgtt caacattaac tcatcggatc agttcagcaa ctattgcatt agctaacaat
     7921 aaaaatgaaa atgatttcca cgatacagaa aaaggaggct gtcatggtcg cacccattcc
     7981 cgcgaaacgc ggcagaaaac ccgccgttgc caccgcacca gcgactggac aggttcagtc
     8041 tttaacgcgt ggcctgaaat tactggagtg gattgccgaa tccaatggca gtgtggcact
     8101 cacagagctg gcgcaacaag ccgggttacc caattccacg acccaccgcc tgctaaccac
     8161 gatgcaacag cagggtttcg tgcgtcaggt cggcgaactg ggacattggg caatcggcgc
     8221 acatgccttt atggtcggca gcagctttct ccagagccgt aatttgttag cgattgttca
     8281 ccctatcctg cgcaatttaa tggaagagtc tggcgaaacg gtcaatatgg cggtgcttga
     8341 tcaaagcgat cacgaagcga ttattatcga ccaggtacag tgtacgcatc tgatgcgaat
     8401 gtccgcgcct atcggcggta aattgccgat gcacgcttcc ggtgcgggta aagccttttt
     8461 agcccaactg agcgaagaac aggtgacgaa gctgctgcac cgcaaagggt tacatgccta
     8521 tacccacgca acgctggtgt ctcctgtgca tttaaaagaa gatctcgccc aaacgcgcaa
     8581 acggggttat tcatttgacg atgaggaaca tgcactgggg ctacgttgcc ttgcagcgtg
     8641 tattttcgat gagcaccgcg aaccgtttgc cgcaatttca atttccggac cgatttcacg
     8701 tattaccgat gaccgcgtga ccgagtttgg cgcgatggtg attaaagcgg cgaaggaagt
     8761 gacgctggcg tacggtggaa tgcgctgact ttttctggcg ggcagaggca atattctgcc
     8821 catcatacct gagtggcaat agaataaggg tgtctgttaa tcgcattgac gccaaaataa
     8881 cttaatgtca tacacttcac acacttttat taactcagca ttatttttaa acatcaaact
     8941 acttaatcat taacaaaatc gcaagatctc attccaccga aacttttacg gctgcattat
     9001 gagattgagc gatttatgat atttctttag ttgtaatcat attaaatttt ttcactgttc
     9061 tgatagttaa aattcaagac atcaataaac aatgagatat ttaaatgatt actcgtatcc
     9121 ctcgtagttc tttctctgca aatattaata atacagccca gacaaatgaa caccaaaccc
     9181 tgagtgaatt gttttataaa gaactcgagg ataaattttc tggcaaggag ctggcgacgc
     9241 ctctattaaa aagcttctca gagaactgta gacaaaatgg tcgtcatatc tttagcaaca
     9301 aggattttgt cattaaattt tccacgtccg tcttacaagc tgataagaaa gaaattacga
     9361 taattaataa aaacgaaaac acgacactta ctcaaaccat tgccccaata tttgaagaat
     9421 acctaatgga aattttacct caacgctcag acactcttga taaacaagaa ttaaacctaa
     9481 aatcagatag aaaagaaaaa gaattcccaa gaattaaact taatggtcaa tgttattttc
     9541 cggggcgacc ccaaaaccgt atagtatgcc gacacattgc tgcacaatat attaatgata
     9601 tttatcagaa tgttgattac aaaccccatc aagatgatta ctcttcagct gaaaaatttc
     9661 tcacgcactt caacaaaaaa tgcaaaaacc agactttggc gttggtttcc agccgtcctg
     9721 aggggcgttg cgttgctgcc tgcggtgatt tcgggctagt tatgaaagca tattttgaca
     9781 agatggaatc aaatggcatc agtgttatgg cagccatatt actggtggat aaccatgctt
     9841 tgacggtccg gctaagaata aagaacacaa ctgaaggatg tacccattac gtggtttcgg
     9901 tttatgatcc taatgtaact aacgataaaa taagaattat gagcgaaagc aaagaggata
     9961 ttaaacacta ttctctgatg gattttatga atgtagatta tagcctcctg aaatggtcaa
    10021 atgatcatgt tattaaccaa tctgttgcaa taattccagc acttccgaaa gaacagctat
    10081 tgatgttaaa aggatctgtg gatgaaataa cccctccatt atcaccagca acgatgaatt
    10141 tgctaatggc aattggtcag aatcaccaac ttacgcaact gatgattcag ctccagaaaa
    10201 tgccagaact acatagaaca gaaatgttga ctgcctataa tagtggacat atgaacgtta
    10261 ttaatactat ttttaacgca ttacccactc tgtttaatac gtttaaattc gataaaaaaa
    10321 atatgaagcc cctcctcctg gcaaataatt ctaatgaata tcccggtttg ttttcagcga
    10381 tacagcataa acaacaaaat gttgtagaga cggtttatct tgctttatct aaccatgcac
    10441 gcctgtttgg atttaccgct gaagatatta tggatttttg gcaacacaaa gccccacaaa
    10501 aatactctgc ctttgagttg gcttttgaat tgggtcaccg ggttattgct gaattaatcc
    10561 ttaatacatt aaataagatg gctgaaagct ttggctttac ggataaccct cgatacattg
    10621 cggagaaaaa ttatatggaa gctttactca aaaaagcatc tccccatacc gtacgctaaa
    10681 tctttgcctg cgccgatacg cataaacatc ttccacatgc ccttcacgta tgcggttttg
    10741 tagtgcgcgc cagtaatcag cgcggaacag gtcggcgtgc atctcttcaa acagcggacc
    10801 aatacgcggg tcggcgcata gccagtggcg aaactcttcc gggaaaacat cgcccggcga
    10861 gacgctgtac cacggttcgc tggcaagttc gtcttccgga tagcgcggcg gcgggatgtc
    10921 gcggaaattc acttccgtca tgtagcaaat ttcatcgtaa tcataaaaaa ccacacgccc
    10981 gtgacgggtg acaccgaagt ttttaaacag catgtcgcca ggaaaaatgt tagcagcggc
    11041 aagctggcga atagcgttac cgtattcttc aatggcgtcg cgcaactgct gaccttccac
    11101 ttgttccagc cagatattga gcggcaccat ccgccgctcg atatagagat ggcgaattac
    11161 aatctgctca ccaagatcgg tgattttttc cgctgcttcc tggagcagta attccattaa
    11221 tgccggggaa atatgccgct tctccagcac aaagttttca aactcctggg tgtccgccat
    11281 tcggcccaca cggtcgtgct ctttcaccag ttgatagcag gcacgaacgt gagcggcaga
    11341 catctctttc tgcggcgcga acttgtcttt gatgactttg aatacccgat caaagcccgg
    11401 cagcgtaaat accaacatca ccattccacg aatacccggc gcttcaatga actgctcatt
    11461 acagccctgt agataaacga gatattcgcg gtagctttcg gttttggcgt gcttctggca
    11521 gccgatagcc atatacaatt cagcagtggt tttacctagc agaatttccc gtagccactc
    11581 gaccagcgct gcgggcagcg gtgcataaac cataaaataa gaacgcgcaa agccaaaaac
    11641 aatgctcgct tcggcggtcg tcgtcaggca ggtatcaata aataactcgc cgtcgtccgt
    11701 ctggtggatc ggcagtaaga agggcaacgt gccggaaggc gtgatcagtt tgcccaccag
    11761 ccaggcggct ttattgcggt aaaacagttc gttcgccacc tgtaaatgac tttccgcgag
    11821 gttgtctgtc cccagcgttt ccgtcagatg gcgaatgatg taatgaatgt cacggctttt
    11881 attctgccag cgcaggcgca gcggcaggtc gctgataacg cgcatcagta gagattccca
    11941 gccgtgatcg gggtgaaagt ctttcgccag cgggcgggga atggtacgaa agcggcgctc
    12001 tggctgagag ctaaagataa aaagccgctc gggagtaagc gagcggtggt caaataaccg
    12061 acagtacacg gagttaaaaa agctctccgc aatctcgaag cgcgggtaat ccggcaacag
    12121 ccgcgtgtaa tgctctttaa cgcgcagtaa aaatgccgcg tccgtacttt ggccgttagt
    12181 aatgcagcgc agttgctcca cgaccagacc aacgtgatga tcgtaaagat ggatacggtt
    12241 tttcatcgcc tgctggacag catgccagtc ggcctgttcg aaacgctgct gcgccccgga
    12301 agtcacttcg aggaatcgac catactgagc gtcgaagcct tgcaaaatgg tttgagcaat
    12361 cagtaattcc aggccacgcg gcatttagcg ccctcatcag gagcagagaa ttgccggatg
    12421 cggcgtaaac gccttatcca gcctacgttc ggcaacggtt gtaggcatga taagacgcgc
    12481 aagcgtcgca tcaggcatcg tgcaccgatt gccggatgcg gcgtaaatgc cttatccggc
    12541 ctacgttcgg caacggctgt aggcatgata agacgcgcaa gcgtcgcatc aggcatcgtg
    12601 caccgattgc cggatgcggc gtgaacgcct tatccggcct acagtcagca acggttgttg
    12661 ttgcttagaa ctgcgattct tcagtggagc cggtcagcgc ggtgactgaa gacgtgccgc
    12721 cctgaataat agtcgtcact ttatcgaagt aacctgtacc cacttcctgc tggtgagata
    12781 cgaaggtata gccatctttc gcggcggcaa attccggctg ctgcactttc tcaacgtagt
    12841 gcttcatacc ctcgccctgg gcataggcgt ttgccaggtc aaacatgttg aaccacatgc
    12901 tgtggatacc tgccagggtg atgaactgga acttgtagcc catatccgac agctgctgct
    12961 ggaagctggc aatagttttg tcgtcgaggt ttttctgcca gttgaacgac ggcgagcagt
    13021 tataagccag cagtttgccc ggatatttcg cgtggatagc ttgtgcaaag cgacgcgcca
    13081 gttccagatc cggcgtggag gtttcacacc agaccaggtc agcatatggc gcatacgcca
    13141 ggccacggct gatcgcttgc tcaatgcccg catgagtacg gaagaagcct tcactggtac
    13201 gctcgccagt aataaattca ctgtcatacg ggtcgcaatc ggaggtaatc agatccgccg
    13261 catcggcatc ggtacgagca accagcaggg ttggaacgcc cgtcacgtca gctgcaagac
    13321 gcgccgcgac cagtttctga atagcttcct gagttggcac caaaactttg ccgcccatgt
    13381 gaccgcattt cttcactgac gccagctgat cttcgaagtg aactgccgct gcaccggctt
    13441 caatcatcgc tttcatcagt tcaaaggcat tcaggacacc gccaaaaccg gcttccgcat
    13501 cggcaacgat cggcaggaag taatcgacat agcgcggatc gcccggctca atgcctgcgg
    13561 accattggat ctgatcggca cgacggaagg tgttgttgat ccgctccacc acagctggca
    13621 ccgagtttgc cggatagagc gactgatccg gatacatgct ggccgccagg ttagcgtccg
    13681 ccgctacctg ccatcccgac agatagactg cttcaatacc cgctttcgcc tgttgcagcg
    13741 cctgaccgcc agtcagtgca ccgaggctgt tgatgtagcc ttttttcgac tcgccatgca
    13801 gcagacgcca cattttcgcc gcgcccagtt gcgccagggt gcattcagga ttgactgaac
    13861 cgcgtaattt caccacatct tccgcgctgt atgggcgagt aatgccttcc caacgcggtt
    13921 gagtccactc tttctgtaat tcttcaattt gttgtgtacg ggttttcatg tgcagatgct
    13981 ccatagttat gtggtggttt acgctaacag gcggtagcct ggcagggtca ggaaatcaat
    14041 taactcatcg gaagtggtga tctgttccat caagcgtgcg gcatcgtcaa aacgcccctg
    14101 ggaaaaacgt tcttcgccca gttcgctggc aatgactttc atctcttcac ccagcatctg
    14161 gcggaacaag gctttggtca ccggtttgcc attgctcaac gttttttgat gatggatcca
    14221 ctgccagatt gaggtacggg aaatttcagc cgtcgccgca tcttccatca ggccataaat
    14281 cggcacgcag ccgttgccgg agatccatgc ttcgatgtac tgcactgcca cgcgaatgtt
    14341 ggcgcgcata ccttcttcgg tgcgttcacc atcacaaggt gccagcagct gatcggcagt
    14401 aatcggcgcg tcttgttcgc gcatcacttc aagctgattt ttacgggagc cgagaatgtc
    14461 gttgaatacc gccattgccg tatccgcaag gcctgggtga gcaatccatg tgccatcgtg
    14521 accgttattg gcttccagcg ctttatccgc ttttactttg ttgagcacct ggttattgcg
    14581 ctcttcatct ttgctcggaa taaacgctgc catgccgccc atcgcaaaag caccgcgttt
    14641 atggcaggtt ttaatcaaca ggcatgagta agcattcagg aatggtttat ccatcgtcac
    14701 tgcctgtctg tctggcagga cgcgatcggg atagtttttc aacgttttga tatagctgaa
    14761 gatgtaatcc caacgaccgc agttcagacc aacaatatgg tcacgcagcg cgtgaaggat
    14821 ttcatccatc tggaacacgg cgggcagcgt ttcaatcagc aacgtcgcct tgatggtgcc
    14881 gcgcggcaga ttaaagcgat cttctgcata gctgaagact tcgctccacc aggccgcttc
    14941 ctgccaggac tgggttttcg gcagatagaa atagggaccg ctgccctttg ccaacagtgc
    15001 ctgatagttg tggaagaaat agagcgcaaa atcaaacagg ctaccgggga ttgcctcacc
    15061 acgccaggtg acatgttttt ccggcaagtg cagaccgcgt acccgacaaa tcaaaaccgc
    15121 tggattgggc ttgagctgat aaattttgcc tgcttcattg gtatagctga tggtgccgtt
    15181 aaccgcatcg cgcaggttaa tttgcccgtc gatcactttg ttccagtctg gtgccagtga
    15241 atcttcgaaa tcggccataa agactttcac attggcgttc agtgcgttga tcaccatctt
    15301 gcgctcaacc gggccagtta tctcgactcg acgatcttgt aagtccgcgg gaataccacg
    15361 aattttccag tccgtatcgc gaatggaagc catttccgaa ataaaatcag gcaaagttcc
    15421 gttatcaata tcttgctgct gctgaatgcg cgctgccaga agtttattgc gttgtggcgt
    15481 aaaatgcgtc accagctcag tcagaaattc taccgcttcg gcagtaagaa tttgcttctc
    15541 ctgctcgcca tacggcctta tgaaagccag ttcatcggtt gttgttgcct gttcagtcat
    15601 cgtgcagctc ctcgtcatgg atccgaaaac ttccccactg tgaacgaagg atcgttgtgc
    15661 gcttttcgtt cagcacaact aagactactc atttaaaatg caaaatcaaa aacaatttcc
    15721 attttaatta aaaatacttg ataacttatt gataacaata tgaataaaat ttattcacct
    15781 cctgaggtgc gtttcggaaa taaaaaaggc acccgaaggt gcctgaggta aggtgctgaa
    15841 tcgcttaacg atcgactatc acagaagatt aatccagcgt tggattcatg tgccgtagat
    15901 cgtatggcgt gatctgatag acgtaatagt tgagccagtt ggtaaacagc aaattaccgt
    15961 gactacgcca gctcgctcgc ggtgtatttt gcggatcatt gtgcgggaaa tagttatacg
    16021 gtacttccgg gcctagtccg gcttccacat cgcggaaata ttcctgcgcc agcgtttgcg
    16081 catcatattc gggatggccc gtcacaaagg caatgcgctt atctttgctg gcaaacagat
    16141 atgcatcccc ttcttccgtc tctgccagaa tttccagatc ggtgtaatca cgaatcaacg
    16201 ctgccggaaa gtcagcatag cgcgaatgtg gtgccaggaa tgaatcatca aagccacgcg
    16261 tcagaagcgc atgaggatgg agaatatgat gctcgtaaac gccagagagt ttgtcggtgc
    16321 gagtttgctt aggaatgccg tagaggatat tgagcgcggc ctgtaccgcc cagcagacaa
    16381 acagcgtcga ggtgacgtga tctttcgacc actccagcac ctgtttgatc tgcggccagt
    16441 aagcgacatc attaaactcc accaggccca gcggcgcacc ggttacaatc agaccgtcaa
    16501 agttctgttc ctgaatatct tcaaagttac agtagaagtt gttcagatgc tctgcgggcg
    16561 tgttgcgcga ttcacgggaa tcgatgcgca acagctgaat atcgacctgc aaaggtgagt
    16621 ttgaaagcag gcgcagaaac tgattttcag tttcaatctt cttcggcatc aggttaagga
    16681 tcagaacctt aagtggacga atttcctgac cagacgcacg agaagttgtc atcacaaaga
    16741 cgttttcttc acgcaagaaa ttgacggcgg gtagctcgtc cggcacacga ataggcataa
    16801 cctgattacc tcactacata cgcttatacg tttagacatc cagatagctg aagataacca
    16861 gaaaatttgc caatgtcgag cctgcatgtt gaaggtgaga aagtttcaag gaaaaggcta
    16921 cgttagaata taagaatgac gaaaaggaga gaaaatggtt attagtattc gccgctcaca
    16981 gcatgaggaa ggggaggaac tcgttgcgat ttggtgtcga tctgtcgatg ccacccacga
    17041 ttttttatca gcagagtatc gggccgagct ggaagagctg gttcgttcct ttctgccgga
    17101 agcaccgttg tgggtcgcgg ttaatgaacg ggatcagccg gttgggttta tgttgttaag
    17161 tgggcagcat atggatgcgc tgtttatcga tcctgatgtg cgcggctgcg gcgtgggtcg
    17221 gatgctggtg gagcatgcgc tctcgatggc accggaactg acaaccaacg ttaatgagca
    17281 aaatgagcag gcggttgggt tctataagaa ggtgggtttt aaggttacgg gacactctga
    17341 ggtggacgat ttggggaaac cgtatccgtt gctgaatctg gcgtatgtgg gggaataagc
    17401 cgcccctgcc gtgcctgccc aggctggaga agacgcgcca gcgtcgcacc
//
LOCUS       DAZFQD010000111        17430 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.111, whole genome shotgun sequence.
ACCESSION   DAZFQD010000111 DAZFQD010000000
VERSION     DAZFQD010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17430
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.111"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(100..384)
                     /gene="ppnP"
                     /locus_tag="ACHKTW_003713"
     CDS             complement(100..384)
                     /gene="ppnP"
                     /locus_tag="ACHKTW_003713"
                     /EC_number="2.4.2.1"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HFO6464652.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
     gene            complement(456..1133)
                     /locus_tag="ACHKTW_003714"
     CDS             complement(456..1133)
                     /locus_tag="ACHKTW_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HFO6464653.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNQLAHVSRRKVERDLQGVVEVLDNQGYDVILLMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEEMLTVQAQKWQILQKSPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFHQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            complement(1391..1582)
                     /gene="yaiA"
                     /locus_tag="ACHKTW_003715"
     CDS             complement(1391..1582)
                     /gene="yaiA"
                     /locus_tag="ACHKTW_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HFO6464654.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPESLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            complement(1632..2156)
                     /gene="aroL"
                     /locus_tag="ACHKTW_003716"
     CDS             complement(1632..2156)
                     /gene="aroL"
                     /locus_tag="ACHKTW_003716"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HFO6464655.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAPSTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            complement(2339..2797)
                     /locus_tag="ACHKTW_003717"
     CDS             complement(2339..2797)
                     /locus_tag="ACHKTW_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HFO6464656.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNPRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            2917..3726
                     /gene="proC"
                     /locus_tag="ACHKTW_003718"
     CDS             2917..3726
                     /gene="proC"
                     /locus_tag="ACHKTW_003718"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFO6464657.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            complement(3743..4858)
                     /gene="adrA"
                     /locus_tag="ACHKTW_003719"
                     /gene_synonym="dgcC"
     CDS             complement(3743..4858)
                     /gene="adrA"
                     /locus_tag="ACHKTW_003719"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HFO6464658.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMIMCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVVYPLLFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVREGLNTLRLPNTPQVTLRISVG
                     VAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
     gene            complement(4960..5280)
                     /gene="psiF"
                     /locus_tag="ACHKTW_003720"
     CDS             complement(4960..5280)
                     /gene="psiF"
                     /locus_tag="ACHKTW_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HFO6464659.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQSLKGDDRNKFMSACL
                     KKAA"
     gene            complement(5399..6814)
                     /gene="phoA"
                     /locus_tag="ACHKTW_003721"
     CDS             complement(5399..6814)
                     /gene="phoA"
                     /locus_tag="ACHKTW_003721"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HFO6464660.1"
                     /translation="MKQSTIALALLSLLFTPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTGDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTGVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVSDAASLNSVTEANQQKP
                     LLGLFADGNMPVRWLGPKATYHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKAIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKDGNTLVIVTADH
                     AHASQIVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            complement(6915..7175)
                     /gene="iraP"
                     /locus_tag="ACHKTW_003722"
     CDS             complement(6915..7175)
                     /gene="iraP"
                     /locus_tag="ACHKTW_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HFO6464661.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLKHPRQ"
     gene            complement(7357..7560)
                     /locus_tag="ACHKTW_003723"
     CDS             complement(7357..7560)
                     /locus_tag="ACHKTW_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6464662.1"
                     /translation="MLQSSADEACNVLVKKVRYLKDVRKITKMMNGKLIYSWLKWPCG
                     EFFSLNYKLTKRIYFITFIYNKG"
     gene            7638..8732
                     /gene="ddlA"
                     /locus_tag="ACHKTW_003724"
     CDS             7638..8732
                     /gene="ddlA"
                     /locus_tag="ACHKTW_003724"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFO6464663.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLLTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(8756..8968)
                     /locus_tag="ACHKTW_003725"
     CDS             complement(8756..8968)
                     /locus_tag="ACHKTW_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HFO6464664.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGLLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            9228..9536
                     /locus_tag="ACHKTW_003726"
     CDS             9228..9536
                     /locus_tag="ACHKTW_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HFO6464665.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLVGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(9595..10689)
                     /gene="yaiW"
                     /locus_tag="ACHKTW_003727"
     CDS             complement(9595..10689)
                     /gene="yaiW"
                     /locus_tag="ACHKTW_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HFO6464666.1"
                     /translation="MSRVNHLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVCSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAGKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(10702..11922)
                     /gene="sbmA"
                     /locus_tag="ACHKTW_003728"
     CDS             complement(10702..11922)
                     /gene="sbmA"
                     /locus_tag="ACHKTW_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706191.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HFO6464667.1"
                     /translation="MFKSFFPKPGAFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFAFFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIVGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDSVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            12274..13431
                     /gene="ampH"
                     /locus_tag="ACHKTW_003729"
     CDS             12274..13431
                     /gene="ampH"
                     /locus_tag="ACHKTW_003729"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HFO6464668.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITRPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPGIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(13432..14055)
                     /gene="iprA"
                     /locus_tag="ACHKTW_003730"
     CDS             complement(13432..14055)
                     /gene="iprA"
                     /locus_tag="ACHKTW_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide resistance inhibitor IprA"
                     /protein_id="HFO6464669.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNSEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     ITLIEQNQLWRDAFYWLAWQNRILELRDVQLIGHNSYEQIRATLLSMIDWNEELRSRI
                     GVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAINRLPSEY"
     gene            complement(14143..17121)
                     /gene="ehaB"
                     /locus_tag="ACHKTW_003731"
     CDS             complement(14143..17121)
                     /gene="ehaB"
                     /locus_tag="ACHKTW_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin EhaB"
                     /protein_id="HFO6464670.1"
                     /translation="MHSWKKKLVVSQLALACTLAITSQANAATNDISGQTYNTFHHYN
                     DATYADDVYYDGYVGWNNYAADSYYNGDIYPVINNATVNGVISTYYLDDGISTNTNAN
                     SLTIKNSTIHGMIYSECMTTDCAERANDYYHDRLALTVDNSTIDDNYEHYTYNGTYNN
                     AADTHVVDVYNIGTAITLDQEVDLSITNNSHVAGITLTQGYEWEDIDDNTVSTGVNSS
                     EVFNNTITVKDSTVTSGSWSDEGTSGWFGNTGNASDYNGGDWNRDDIALAVIAHPAAD
                     NAMQTTATFDNSTLMGDVFFSSNFDENFFPHGRDSYRDADGDVDTNGWDGTDRLDLTL
                     NNGSKWVGAAMSAHLTVDTNDDGVPDAYGPVYNDNGVVIDTSTGTEATATLIDIAANS
                     LWPSSTVGVENSDSEYSEFDHIIGNEVYQSGLFNVTLNTGSQWDTTKTSLIDTLSINS
                     GSTVNVADSTLISDSISLTGLSALNINEDGHVATDSLTVDNSTVTISDEVSAGWAVGD
                     AALYANNIKVTNDGILDVGNTAANALQVDTLNLTSTTDTSGNIHAGVFNIESNRFVLD
                     ADLTNDRTNDTTKSNYGYGLIAMNSDGHLTINGNGDNDNTASIEAGQNEVDNNGDHVA
                     AATGNYKVRIDNATGAGSIADYNGNELIYVNDKNSNATFSAANKADLGAYTYQAEQRG
                     NTVVLQQMELTDYANMALSIPSANTNIWNLEQDTVGTRLTNSRHGLADNGGAWVSYFG
                     GNFNGDNGTINYDQDVNGIMVGVDTKIDGNNAKWIVGAAAGFAKGDMNDRSGQVDQDS
                     QTAYIYSSAHFANNVFVDGSLSYSHFNNDLSATMSNGTYVDGSTNSDAWGFGLKAGYD
                     FKLGDAGYVTPYGSISGLFQSGDDYQLSNDMKVDGQSYDSMRYELGVDAGYTFTYSED
                     QALTPYFKLAYVYDDSNNDNDVNGDSIDNGTEGSAVRVGLGTQFSFTKNFSAYTDANY
                     LGGGDVDQDWSANVGVKYTW"
BASE COUNT         4422 a         4403 c         4126 g         4479 t
ORIGIN      
        1 atcggaaatt gaaaaaatcg cagcaaagga aatattatta tgggttaaag agaggatatt
       61 tcagaaaaat gccctcccgt tcaaggggaa ggcgaggaat tacagatagc ggcacagata
      121 agaggtgggt tcggcaactt gcagatgaaa ctcactgtga ccgggaacat taaacaccga
      181 accggcttca tacacctgcc agtcggtcgc gtcaggcagt aacacattca gcgcgccact
      241 gattaccgtc atctcttccg gctcagcggt gctgaaggtg tattcgcctt caaccataac
      301 acccacgctg gcgcgaccag tgctgctgct ggaaaagccg attgatttca ctttgccgga
      361 aaagtactca ttactttgaa gcataaactg gccctgtctg gtagttgaac atctacttca
      421 ctataggggc cagaggcgct ggctgtcacg caaaattaca ccagtaattc cgcagccagc
      481 cgtgcaatca atacgttaga cagcaagaca ggaacatcga gctgtttttg cagtaaatcg
      541 cgatgacgtt ggtgaaatcc taaacaatcc agcatgatga catcagcccc ttttgccagt
      601 aattctttcc cggcatcaat gattttttgt tctgaatcat gaatggggtt gcccaatgaa
      661 aataccggcg atttctgcaa aatttgccat ttttgcgcct gaacggtcag catctcctca
      721 accggaacga taacccccac ctgatgatct tcaacaatag aggaaaccag tggaggcaat
      781 attcgcgacg gctcaagaaa gatcgtatta cgcgcagtca tactactaat gtttgctgta
      841 ctcattaata aaatgacgtc ataaccctga ttatcgagca cttcaaccac accttgcagg
      901 tctcgctcca ctttacgacg cgaaacatgg gccagctggt tgtcatttaa taatgtgaga
      961 atggtgtctt cgcctgcttc tggcgcgtac tccgccatca cttcttcacg acttaacttc
     1021 cccagcaagc tatgatggga aatattatct tcgtcaatgt attccgtcag gagcggcaaa
     1081 acctcctgca taggtacaat gccgatggtc aggatcgcca acgacgcact cattgttcat
     1141 ccaccttact tttctttcgt cgttaattac cgggcaagtg tgaagcacca ctgctgacac
     1201 ttacttgtgc tacaaatgac aaaaagcgta gcagcagacg tgcacggcat aatgtaaaga
     1261 ttccaaatga ttccagtaat ggattgttat tgtttaatat tctaattatt agaaaaacat
     1321 gaattatgaa aaatgtgacg cagatccctt gtagtggcac tcagaatccc ttccgagcag
     1381 tctgatgcgg tcactctttg tcagggtcct catagcgttt tttcgcatcc atcgcttcct
     1441 gagccgtcgg atgttcactg atcaacgact ctggtttagg atgatcggct ctgagttggt
     1501 accaggttac cgtctgtcct ggctttcctt tttcaatcgt cactatatat gcttcacgag
     1561 gataaggcgg tttcgttggc atcgttcttc cttatttcac gggatgaacg ttaagtatag
     1621 gcgctctaaa atcaacaatt gatcgtctgt gccagggcgc tacgaatttc agaaatcacc
     1681 tggctgggtt cgtttgttgc gtcgatgata atatgcgcaa cttcgcgata tagcgcatcg
     1741 cgttcttcca gcacttcctg aacttcttcg ctcagcggtt ttcccgttaa ggttggccgt
     1801 aaatcttctt ccggtgcagc ttgcagtcgg ttaaccagga ctgatactgg cgcacacaaa
     1861 taaaccacga tcccgttatt ttgcatgaag tgacgattaa attccgtcag aataatgccg
     1921 ccgcctgtag cgataacggt ggatggcgca gttaccgctt ccagcgccgc cgtttctctg
     1981 gcgcgaaatc ccgcccactc ttccctttcg acgatctccg cgaccgtcat attgagctgt
     2041 gattgcaacc actgatcggt atcgacaaac cgacggttaa gcgaatcggc aagggccatt
     2101 ccgaccgttg ttttaccaca gccccgaggc ccgatcagaa aaagaggttg tgtcatcgtg
     2161 ggttttcccc aataggtcgc aatgcggcga aagccggtgt catgagaata gcgatcatac
     2221 catcaaacta gtacaatttc gattgtaaag aaaaaattcc acttaaagtg aaaatctcaa
     2281 tacatccctt actataccaa taaatgttca agaatgaagt gtaaataata aattacattt
     2341 agccacgact acgttgcact tccagccacc acttctccag ctccgcggca aaggcctggc
     2401 ggtcacgttg tgaaaggcta tctggtccgc cggtctggat cccactggca cgtaaggtat
     2461 ccataaaatc gcgcatcgtc aggcgctcac gaatggtcgc tggcgtgtaa cgttcgccgc
     2521 gcggattaag cgccgctgcg cctttctcga tggcttcagc agccaaaggt atatctgcgg
     2581 tgatcaccaa atcgcccgct tcacactgcc ggacaatttc gttatcggca acgtcgaaac
     2641 ctgccgcgac gcgcagcgta cgaataaatc gcgatggcgg cacgcgtaaa ctctggtttg
     2701 ctaccagtac cagcggcatc tgcatacgtt ccgccgcgcg atacaaaatc tctttaatta
     2761 cattgggaca cgcgtcggca tccacccaaa ttgtcataaa gtcatccttt gttgggtaat
     2821 cctctattgt gtcgcgcttt tgccttccgg catagttctg tttatgcttc tgccagcgat
     2881 tatcaaaaca atgaatttca cggcaggagt gaggcaatgg aaaagaaaat cggttttatt
     2941 ggctgcggca atatgggaaa agccattctc ggcggtctga ttgccagcgg tcaggtgctt
     3001 ccagggcaaa tctgggtata caccccctcc ccggataaag tcgccgccct gcatgaccag
     3061 ttcggcatca acgccgcaga atcggcgcaa gaagtggcgc aaatcgccga catcattttt
     3121 gccgccgtta aacctggcat catgattaaa gtgcttagcg aaatcacctc cagcctgaat
     3181 aaagactctc tggtcgtttc tattgctgca ggtgtcacgc tcgaccagct tgcccgcgcg
     3241 ctgggccatg accggaaaat tattcgcgcc atgccgaaca ctcccgcgct ggttaatgcc
     3301 gggatgacct ccgtaacgcc aaacgcgctg gtaaccccag aagataccgc tgatgtgctg
     3361 aatattttcc gctgctttgg cgaagcggaa gtaattgctg agccgatgat ccacccggtg
     3421 gtcggtgtga gcggttcttc gccagcctac gtatttatgt ttatcgaagc gatggccgac
     3481 gccgccgtgc tgggcgggat gccacgcgcc caggcgtata aatttgccgc tcaggcggta
     3541 atgggttccg caaaaatggt gctggaaacg ggagaacatc cgggggcact gaaagatatg
     3601 gtctgctcac cgggaggcac caccattgaa gcggtacgcg tactggaaga gaaaggcttc
     3661 cgtgccgcag tgatcgaagc gatgacgaag tgtatggaaa aatcagaaaa actcagcaaa
     3721 tcctgatgac tttcgccgga cgtcaggccg ccacttcggt gcggttacgt ccggctttct
     3781 ttgctttgta aagcgccaaa tctgccgatt tcaaccactc acgatagtga ctcatttgtg
     3841 ggttcagcgg cgcaaccccc acactaatcc gtaaagttac ctgtggcgta ttcggcaagc
     3901 gtaacgtgtt tagcccttca cgcacccgta acatggcggt aatggcgctc tcagctggcg
     3961 taccggacat gattactgca aactcatcgc cgccaaaccg accaatcaca tcgctaccgc
     4021 gcagggtaat ttgtaactgt cgggtaagcg ccacaatcgc ttcatcgccc acatcatgac
     4081 cccaggtatc gttgatgctc ttgaaatggt cgatatcgat aatcagtaac gttgcatcgc
     4141 gattatgccg ccgacagtta tcaaattcat tgcgtaacat agtttcccaa tgacgtcggt
     4201 tatacacgcc cgtcatgccg tcgcgggtac tcatgacctg caacctgcgt ttatgttccg
     4261 ccagtttggt tgccgtctgg tagctgaccc agccaaacag cagaggataa acgacaataa
     4321 tgggaaggga gagccaccat tccagcggcg cactattgaa cgacacggta atgcccgtaa
     4381 gctcgagggt gacaaggcag gaaaccacca tcaacaccag acccgcgaca aacagacggg
     4441 ggccgcctgc ccccatcaaa ttcagacaca taatcatcaa catagcggtg gaaggcagca
     4501 cgtttacgcc cattacgcct acccacattc ccgctaatac tgcatcggtt tttaagttgt
     4561 aaatttcccg gctaagcgga tcgacggccc tgctcgctat ctgccaggct aaatgcggcc
     4621 agacgaacgc ccagccgacc aacaccagcc accaccagcc cggcggcggg tgcgaaacca
     4681 gcgttgaagc aatcggtaag aacatgccag ccaggccaac cgcacggggt agtctgacgc
     4741 gacgggcgaa gcgcagcccg gaccgctgat gttcgttttg aggagttaaa ggaggttcct
     4801 ccccatgcgc cgccgctttt ttgaaaaagt tttcatcatt cattattttt gggaacattc
     4861 agaaacaatt ttcccaaatt atagagacgg ggctaaaggc agaagtatga aatttcgggt
     4921 gagccacttt cgtagctcac ccgttagcac tatcaggtat caggcggctt tcttgaggca
     4981 ggcactcata aacttattac gatcatcacc tttcagagat tgttgcgttg cttgattatt
     5041 acattcgcgc atcttttgct gctgtggcgt caaacttttt tcaccaggcg cagacttgct
     5101 gttcttcagg caatcactca tgtaggtctt acgagcatcc cctttcaacg cctgcgccgt
     5161 cgcctgctga ttacaggatg tcatacgctg ttgttgtggg gttaaggttc tctcggcagc
     5221 gccgacggtg gttaaaaaaa ccagaccaaa aagcaaggta accagtaatg ttattttcat
     5281 agcaccatcc ctcttcatgt tttaaccatg agcgtatgcg cccgtgatct gccattaagt
     5341 ctggttgcta acagcaaaaa aaccacccgg cagcgaaaat tcactgccgg gcgcggtttt
     5401 atttcagccc cagggcggct ttcatggtgt agaagagatc ggtctggtcg gtcagtccaa
     5461 caacattggc ggcatgcggg ccatacgccg caatacgcaa ctgactgccg gtatgttctt
     5521 gtgaatcctc ttcggagttc ccgtagctca tcaccatcac tgcgccatct ttggtattta
     5581 gcgcctgggt gaggcccgga gctttggtat ccggcgcgac aatctggctg gcgtgggcgt
     5641 gatcagcggt gactatgacc agcgtattgc catccttttt agcgaattcc agcgcccgtt
     5701 gtacggcttc atcgagatcg accgtctcgc caatttgccc acaaggattc gcagcgtgat
     5761 cctgtttatc gattgacgca ccttcaactt gcaggaaaaa tcctttctca tttttactca
     5821 acaattcaat ggctttgtcg gtcatctgcg ccagggttgg tacactgtca ttacgttgcg
     5881 gattaggcgt acaggtgact gcgggcttat cgatattgcc gtggtacgtt gctttcggtc
     5941 ctagccagcg cactggcata ttgccgtcag caaacagtcc aagcagaggt ttttgctgat
     6001 tcgcttccgt caccgaattc agtgaggcag catcgctgac caactgataa ccacgcgcct
     6061 gtgcctgttc acgcagcgtt tttccctgcc attcaccggc ggttgccgtt tcagcaaagg
     6121 tttttgcgcc accgccaagc gtaacgtcgg cacgagcgtt aagcagctgt tcggtaatcg
     6181 atccttttcc gcctttttcc agagcgttac ccggacattt ttcactggtc gcgctcggac
     6241 cgtagcattt gcgcgaagtc acatgtgcca ccagcgcagc gggcgtggca tcctgcaact
     6301 ctgcggtaga aacgttaccg gtcgccagac ctgcggcttt tgccatttcc agaatcgttg
     6361 ggtgatcttt ttcgtgaata tcgacgccca gcgcgccgtt ataggttttg acaccggttg
     6421 accaggcggt tgctgatgca gccgaatcgg tgacgtagtc cggtttgccg gtttttttat
     6481 tcagcgcata gtgagtgtat tgcccggtaa gcggtaaggc atcgatacct ttaaaaaagc
     6541 cgcccgcacc ttcggcataa ttacgtgcgg ctgtaatttc cgaatccccc atcccatcgc
     6601 caatcagcaa aataatattt tttgcaggtt tatcgctaag agaatcacgc agagcggcag
     6661 tctgatcacc cgttaaacgg cgagcaccgc cgggtgcagt aatatcgccc tgagcagccc
     6721 ggttttccag aacaggcatt tctggtgtcc gggcttttgt cacaggggta aacagtaacg
     6781 ataagagtgc cagtgcaata gtgctttgtt tcactttatt ttctccatgt acaaatacat
     6841 taaaaaataa aaacaaagcg actataagtc tcggccgtga caactttatg acagctgttg
     6901 aaaagattaa ctttttactg acgaggatgc ttcaataact tctttacgta atcgcgcagc
     6961 agctccgtat cgtcgtcagg aatgctggca tcgggcttta cctcgtacag cgccccctct
     7021 acctgatcaa tcaaccgctg ttggtcattt tgcgccatat tgcgaagcat tgcagtgacg
     7081 ataatctcca aagcttctac ctgcgcacac agttctttcg actcttcttc tttttgggca
     7141 agcttaaata acaactcagc aatgagattt ttcatgtctg tatttcctta tccaaagtat
     7201 ggagaagtta acattatgtt tcacaacttc atagtgtctg gaagtattat ttttatgaca
     7261 aaggaaatac tttgcgcaaa tattttttgt ttgattacca gcgaaacgtt tctcttattt
     7321 aaataattac ccacacaaaa tataggcttt taattatcaa cctttattat aaataaaagt
     7381 tatgaaatat attctcttcg ttaacttata attaagagaa aaaaactcac cgcatggcca
     7441 tttaagccag gaataaatta gcttcccgtt catcattttt gtgatcttgc gcacatcttt
     7501 gagataacgc actttcttca ccagtacgtt gcacgcttcg tcagcagaag attgcaacaa
     7561 tctttcagaa ggccgcgaat cccccgtttt gtcgtggtaa tgtatgcact ttgtgttaga
     7621 cgggataggt ttttaagatg gaaaaactgc gggtaggaat cgtttttggt ggtaaatcag
     7681 cggaacatga agtgtctctg caatcggcaa aaaacattgt cgatgccatt gataaaagtc
     7741 gcttcgacgt tgtgctgctg ggcattgata aacaagggca atggcacgtc agcgatgcca
     7801 gcaattatct gctaaatgca gacgatcctg cccatattgc gttacgccct tcggcgacca
     7861 gccttgcgca ggtgccaggt aaacatgagc atcagcttat cgacgcgcaa aacggtcagc
     7921 cgttgctgac ggtggatgtc attttcccga ttgtccacgg tacgctgggc gaagatggtt
     7981 ccttgcaggg aatgctgcgg gtcgccaatt taccgtttgt aggttctgat gttctggctt
     8041 cagcagcctg tatggataaa gatgtcacca aacgtctgct acgcgatgcc gggctgaaca
     8101 ttgcgccatt tattactctg acgcgcgcta atcgtcacaa catcagtttt gccgaagtgg
     8161 agtctaaact ggggttacca ctgtttgtaa aaccggctaa tcagggctct tctgttggtg
     8221 tcagcaaagt aaccagtgaa gaacagtacg caattgccgt cgatctggcg ttcgagttcg
     8281 atcataaagt gatcgttgag caagggatca aaggtcgtga gatcgaatgc gcagttctgg
     8341 gcaacgacaa tccgcaagcc agcacctgtg gcgagatcgt actcaccagc gatttctatg
     8401 cctacgacac caagtacatt gacgaagatg gcgcgaaagt ggtagttccg gcagccattg
     8461 caccagagat caacgataag atccgggcga ttgccgttca ggcttatcag acgttgggat
     8521 gcgcaggcat ggcgcgtgta gacgtgtttt taaccccaga gaacgaagtg gtgatcaacg
     8581 agatcaacac actgcctggc ttcaccaaca tcagtatgta tccgaagctg tggcaagcca
     8641 gcggtctggg ttacaccgat ctgatcacac gtttgattga gctggcgctg gagcgtcacg
     8701 ctgcggataa cgcattgaaa accacaatgt aatattttaa cgcccgataa tattcttatt
     8761 gggcgttttc agtttcttca tcgcgacgac gaataatcaa ccccgccagc cagaaactaa
     8821 tcacccacgt caccagaccg acggcatagg tctgccaacc ttttgcttca aatcccagta
     8881 gccccaccac gccattaaga atgaatataa ggccaatggc gaacgcatag tagtgccagt
     8941 cacggcggat ttttacaggc agattcatgg cagctccgtt aacgaaaaac aacatccttg
     9001 catattctgt aacaaccgtc tgtggtgtag acaacatttt tgtaattgtt aataaattta
     9061 acttaaggtt gccattttgt gactcacaac gaagattcac gcttcgggaa tattcccaac
     9121 atcaatttac catgattctg atattgacaa tctaaatgac ctgtcagact tgccataagc
     9181 cgctggtatc acaccagaag agaagcagat gacttccgga ggtttttatg gctgatttca
     9241 ccctgtcaaa atcgctgttt agcggaaaat atcgcaatgc ctcttcaacg cctggcaaca
     9301 ttgcctatgc gttgtttgtg ctgttttgct tttgggctgg ggcgcaattg ctgaacctgt
     9361 tagtgcatgc gcccggcgtc tatgagcgtt taatgcaggt ccaggaaaca ggtcgcccac
     9421 gggtggaaat tggtttaggt gtcggcacca ttttcgggct gatcccgttt ttagtaggct
     9481 gcctcatttt tgcagtggtg gcgctatggc tgcactggcg acatcgccgc cagtaagatt
     9541 tacgttgcca gatacggcct aaaacgcctt atctggcatg acattcctgt ttgatcactg
     9601 tttcatacaa cgcgcccgcc gttcgtctac gcgcttcgca aaccaggccg tagtcaggtt
     9661 gcgggtgatt ttcgggcttt ccagttgaat gccaggcaac atttcgcggg gtagtgattt
     9721 acccgttttc gcttcggcaa gttgataaac tttcttgtac agcgccgtct cctcaaaaga
     9781 gaagctgtca cctttctcta actgacggcg aatttcgctg tcgctcatcc ccagttttcc
     9841 cgccagtttg cgcgttgcca gttcggtttt cccgggttct ttactgtcgt aacgaattaa
     9901 atcaccgtcc agtgccagct tcacgccgct ggccttactg actgcgttct gaaacgcggc
     9961 attacggctg gcgtaccagc cagcgttaaa atcagcaaag cggtatatcg gtgcgctata
    10021 gctggcggga taattcagta aatggtaagt accaaaccac aacccgccgc ggcgactgaa
    10081 gacttcctga cgaactgtac cgtccatttt ccacggatac cctttggtat gctgttcggc
    10141 aaaagcaatg ctgacctgca tcggcccacc ggtgcgcacc ggattaagcg aaccaaacag
    10201 cgtctgcccc attggtacca tgctgatcag atcgtcaaaa atcgcgctca attgcttttc
    10261 tgtacgcacc gaatctaacc gttcgctata acttttgccg tttggcgatt tgatttttag
    10321 cgcggtatga accagaaaag caggaatgtg catccgttca gcacgacggt caatctcttg
    10381 ccaggcgatc ttgcttaagc ccggaacggc cggatctgcc tgataatttg actcctgctg
    10441 cgccaccgcc agcaccgagc agacgttttc cagcgtcggt gccagcccct gactttcaaa
    10501 ggtggtggcg agatctttcg cccaggcatc gcggtctttc acgctcgcag gcatcttctg
    10561 ccgcacgacg ctcgctacat ctatcgcttt ttcgcccttt ttcaacggtt gcggagcctg
    10621 actactacac cccgctaaaa ccagaaccgc caacaatgac agcgaagata gatgattgac
    10681 gcgcgacatt acttctcctt tttagctcaa ggtatgggtt acttcctgaa ttttgtcacc
    10741 atccagctca tgttcaaagc tgcgcagacg tttgtagata gacatcaact caaccagtgt
    10801 ggtccatgag ttaatcaggt actggaaagc accgcgaacc tgaccaaaaa cgttggtaat
    10861 ctgcgtcatc aggccgagcg taatcgtacc ggcaacaatt gacggaaaca gcaagaacaa
    10921 accgaaaacg ctatcgacct gcaaatagag aatgcgggcg atgttgaaat acatatagtg
    10981 aaaatagagg cgaaaatagt ttttccgtac ggcgctaaac agctcgcgta ccgtaggcgg
    11041 cgtcgcgcgc gtggcatcgt cttcaccata aaccagctct ttacggtagg cggcctctac
    11101 tcgctggttt ttaaactcca gccccggcag tttgatccct accactgcca gcaatccggt
    11161 ccccatcagc gaccagacga tagcggcaat caccagacca tacggaatgt gcccgacaat
    11221 tggcagctcc ggcacatgtg cggagagcgt taccagcacc ggcaggaagg cgatcaacgt
    11281 catgatggcg ttgataaaac tgacgcccat attctccagc gttgaagcaa aacgcatggt
    11341 gtcttcctgc acacgctgtg cggccccttc gatatgacgc agttgttgcc agttcgccat
    11401 gtaatattcg ttcatcgccg tacgccagcg gaacacgtag tgactgacaa agaagttgtt
    11461 cagtacactg atcaccaccg cgatcagcgc aatccccaga aagacgccca cttcgcggta
    11521 aaattgttcg atagtgactt tatgcggcga acttagcgcg gtttgaatca gatcatagaa
    11581 cggtgcatac caggcgttga cggcgacccc gacttccacc aaaaaccagg tgacgaagat
    11641 gatcagtgca gtaccgagta ttgaccagta ttgccaacgg tgcgggctgt agataaacca
    11701 gaaaaatgca aaaagtccta cgcaaacaat gtagtaagcg taaaaaatca ggaaatccaa
    11761 cgaccagaaa cgcgcggcgc taatcgggat ctgcccggaa gcgccggtga tacgcgccac
    11821 ccagtctccc ccaccggctt gccagaagat aacggcaatc aatgcccaaa caaaggccga
    11881 gagaaaaaac gctcccggct ttgggaaaaa agacttaaac atatgcactc ctgctaactt
    11941 cttatcgttt taacaactgc tgtgtattaa ccgcatgacc actacaaagc gtcagccaaa
    12001 tgaaaccgcc cattgtgacc catagcaagg cgcttagttc gccagcctga ccataagaat
    12061 tgtttcgacg gatttcacaa accaaagatt gtttacataa gcggattaac acgcgcagga
    12121 tcacaaatgc aggcattggt gagcaggaaa aagatcgtgt ttacgcgccg ttacgttttt
    12181 aacactatta ttgcactaat tcgccctttg caatccatca atgagtagta taaatacgct
    12241 cagttacctt cattcaatct atggacacca ccgttgaaac gtagtctgct tttttctgcc
    12301 gtgctgtgtg cggcgtcatt gacctccgtc catgcggcgc agccgatcac tgaaccggag
    12361 tttgcctctg atattgtcga tcgttatgcc gatcatattt tttacggcag cggtgccacg
    12421 gggatggcgc tggttgttat cgacggtaac cagcgcgtct ttcgtagtta tggcgaaacg
    12481 cgacctggta ataacgttcg cccgcagctg gattcggtcg tgcgtattgc ttccctcacc
    12541 aaactaatga ccagtgaaat gctggtgaaa ttgctcgacc aggggaccgt aaagttaaac
    12601 gatccgctaa gtaaatatgc tccgccaggc gcacgcgtgc cgacctacaa tgggacgccc
    12661 attaccctag tgaatctggc aacccatacc agcgccctgc cccgtgaaca gcccggtggc
    12721 gcggcacatc gtccggtgtt tgtctggcca acgcgcgaac aacgctggaa atacctttct
    12781 acggcgaagc taaaagccgc gccaggcagt caggcggcgt actctaacct tgcgttcgac
    12841 ctgctggcag atgcactggc gaatgcgtcc ggcaagcctt atacccagtt gtttgaagag
    12901 cagattaccc gtccattggg gatgaaagac accacctaca ccccctcacc ggatcagtgc
    12961 cgccgtttaa tggttgctga gcgcggtgcc agtccgtgca ataacacgct ggcggcaatt
    13021 ggcagcggcg gcgtctattc cacgcctggc gatatgatgc gctggatgca gcagtatttg
    13081 tcatctgatt tttatcagcg cagtaaccag gcagatcgca tgcagacgtt gatttatcag
    13141 cgcgcgcagt ttacgaaagt gattggcatg gatgtccccg gcaaagcgga tgcgcttggt
    13201 ttaggctggg tatatatggc cccgaaagag ggtcgtccgg ggattattca gaagacagga
    13261 ggtggtggcg gtttcattac ctatatggcg atgatcccgc agaagaatat tggcgcgttt
    13321 gtggtggtca ctcgttcgcc gttgacgcgc tttaaaaata tgagcgatgg catcaatgat
    13381 ctggtgaccg agctaagcgg gaataaaccg ttggttatcc ccgcatcctg attaatactc
    13441 tgaaggcaaa cggttgatag cgaccagttt gcctttattc atttcgatat agccgccttt
    13501 acgcaaagca gcgagaactt ctgcgacgac agaacgcgat atgcgtgtac gttgatggat
    13561 ataattcatc acaccaatac gtgatcgcaa ttcttcattc cagtcaatca ttgataataa
    13621 tgttgcgcgg atttgttcgt aggaattatg cccaatgagc tgcacgtcgc gtaattccag
    13681 aattctattt tgccaggcta accagtaaaa agcgtctcgc cagagttgat tttgttcaat
    13741 aagcgtaatg gtttgtttgg ctggtagatg atatcccgta caatttcctt ctgatattaa
    13801 tttgtatggt atatcgtttt tcattaaacc atcagccagc cccataatat aaggagcctg
    13861 ggtaataccg ataagtacgt tttcttctct acgcagagag ataacgccct ctaaaataac
    13921 gaaagtatct tcgctattga catcactgga aaatataact tgcttttcat tattaaactc
    13981 gaagcgcgta ccgtatctgg acaaacattt atcgagctta ccaaattcct gaagaggttt
    14041 aactacagat aacatttgcg cgtcctttgc agtaatgccc gtcaaatcct tgacgggcat
    14101 tatttagatt aaattaccag aatttcttcg gagtgaagaa tattaccagg tatatttaac
    14161 acccacgttc gcggaccagt cttgatctac gtcaccacca ccgaggtagt tagcatcggt
    14221 ataggcgctg aagttcttgg tgaagctgaa ctgagtaccc agaccaacac gtaccgcaga
    14281 cccttcagta ccgttatcga tggaatcacc gttcacatcg ttatcgttgt tagagtcgtc
    14341 gtagacgtaa gccagtttga agtacggagt cagagcctgg tcttcgctgt aggtgaaggt
    14401 ataacctgca tctacaccca gttcataacg catgctgtcg taagactgac cgtcaacttt
    14461 catgtcgttg ctcagctggt agtcatcacc agactggaac agaccagaaa tgctgccgta
    14521 aggagtcacg taaccagcat cacccagttt gaagtcgtaa ccggctttca aaccgaagcc
    14581 ccaagcgtcg gagttggtgc taccgtcaac gtaagtaccg ttgctcatgg ttgcagacag
    14641 gtcgttgttg aagtgagagt aactcaagct accatcaaca aagacgttgt tcgcgaagtg
    14701 agcagaagag tagatgtagg cagtctggct gtcttgatcc acctgaccag aacggtcatt
    14761 catgtcacct ttagcgaagc ctgcagccgc accgacgatc cacttagcgt tgttaccgtc
    14821 aattttggta tcaacaccga ccatgatgcc gttaacatcc tgatcatagt tgatggtgcc
    14881 gttgtcgccg ttgaagttac caccgaagta gcttacccat gcgccgccgt tatcagccag
    14941 gccatgacga gagttggtca gacgagtacc aacggtgtct tgttccaggt tccagatatt
    15001 ggtgttcgca gacgggatgc tcagcgccat gttagcgtag tcggtcagct ccatctgttg
    15061 cagaacaacg gtgttaccgc gctgttcagc ctgataggtg tatgcaccca ggtcagcttt
    15121 gttagcagca gagaaggtcg cgttgctgtt tttgtcgttg acgtagatca gctcgttgcc
    15181 gttgtagtca gcgatagaac cagcaccagt agcgttgtcg atacgaactt tgtagttacc
    15241 ggttgcagct gcaacatggt caccgttgtt atcaacttcg ttctgaccag cttcgataga
    15301 agcagtgttg tcgttatcgc cgttaccgtt aatggtcagg tgaccatcag agttcattgc
    15361 gattaagcca taaccgtagt ttgacttggt agtatcgttg gtacggtcgt tggtcaggtc
    15421 tgcatcaagt acgaagcggt tgctttcgat gttgaacaca ccagcgtgaa tgttaccact
    15481 ggtatcagta gtgctggtca ggttcagagt atcaacctgc agagcattcg ccgcagtgtt
    15541 accgacatcc agaataccgt cattagtcac tttgatgttg ttggcataca gagcagcatc
    15601 acctacagcc caaccagcag aaacttcatc agaaatggtt acggtactgt tgtcgacagt
    15661 cagtgaatca gttgcaacat gaccatcttc gttgatgttc agcgcagaaa gacctgtcag
    15721 agagatagag tcagaaatca gcgttgaatc agcaacatta acagttgaac cactgttgat
    15781 gctcagagta tcaatcagag aagttttggt ggtatcccac tgtgaaccgg tattcagggt
    15841 cacattgaac agaccgctct ggtatacttc gttaccgatg atgtgatcaa attcgctgta
    15901 ttcagagtcg ctgttttcta caccaacagt tgatgaaggc cacaggctgt tagctgcgat
    15961 gtcgatcaga gttgcagttg cttcggtgcc tgtactggta tcaataacaa ccccgttgtc
    16021 attgtaaaca ggaccgtaag catctggcac accatcatca ttggtatcaa cagttaagtg
    16081 cgcagacatt gccgcaccaa cccacttgct gccattgttc agggtcagat ccaggcgatc
    16141 agtaccatcc caaccgttag tatctacatc accgtcagca tcacgatagc tatcacgacc
    16201 atgcgggaag aagttctcat caaagttact tgagaagaat acatcgccca tcagtgtaga
    16261 attatcgaat gttgcagtgg tttgcattgc gttatcagca gcaggatgtg caataacggc
    16321 taatgcgata tcatctctat tccagtcacc gccattgtaa tcgctggcat tgccggtatt
    16381 accaaaccaa ccactagtac cttcatcaga ccatgaacca gaggtcacag tagagtcttt
    16441 aacagtgatg gtgttgttaa acacttcgct gctgttaaca ccagtgctaa ccgtgttgtc
    16501 gtcgatatct tcccactcat aaccctgagt caacgtaata cccgcaacgt gagagttatt
    16561 agtgatggac agatcaactt cctgatccag agtaatagcc gtaccaatgt tgtaaacgtc
    16621 tacaacatga gtatcagcgg cattattgta agtgccgtta tatgtgtagt gttcgtagtt
    16681 gtcgtcgatc gttgaattat caacagtcag cgccagacgg tcatggtaat aatcattcgc
    16741 acgttcagca caatcagttg tcatgcactc ggaataaatc ataccgtgaa tagtgctgtt
    16801 tttgattgtc agactattgg cgttggtatt ggtagaaata ccgtcgtcca gatagtaggt
    16861 agaaatcacg ccgttaacag tagcgttatt aatgaccggg tagatatcgc cgttgtaata
    16921 actatcagcg gcatagttgt tccagcctac ataaccatca tagtaaacgt catcagcata
    16981 ggtggcgtcg ttgtagtgat ggaaagtatt gtaagtttga ccagaaatat cgttggtcgc
    17041 tgcattagcc tgagaggtga tagccagagt gcaagccaat gctaattgtg atactacaag
    17101 tttctttttc caggagtgca tttgtcatcc ctcctcaggg acgtaataag ttgatccatc
    17161 aattatcaat gcatagaaaa catattatgt ttcgcgctaa cgattatgct gcgttacaga
    17221 cgaatggttc aattattctt tcccctaagt ccgaatgtgg acatatataa tttatggaga
    17281 aaaacacgca tcaaattgat aaaaggacga tatttcaaca tgttgcagat aatgttattt
    17341 ttgatacata cgcgttataa atatcaaaaa cacaaaccag ataaatatct tatcaatata
    17401 caacaaagtt tgaattattc aatttatagc
//
LOCUS       DAZFQD010000112        17301 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.112, whole genome shotgun sequence.
ACCESSION   DAZFQD010000112 DAZFQD010000000
VERSION     DAZFQD010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17301
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.112"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(61..1101)
                     /locus_tag="ACHKTW_003732"
     CDS             complement(61..1101)
                     /locus_tag="ACHKTW_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFO6464671.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQGGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVMAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(1174..1809)
                     /locus_tag="ACHKTW_003733"
     CDS             complement(1174..1809)
                     /locus_tag="ACHKTW_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HFO6464672.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLTDALAQVTDPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPGHEGVTILSSSELRKRAE"
     gene            1937..2455
                     /gene="yhbO"
                     /locus_tag="ACHKTW_003734"
     CDS             1937..2455
                     /gene="yhbO"
                     /locus_tag="ACHKTW_003734"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HFO6464673.1"
                     /translation="MSKKIAVLITDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGAEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(2435..2878)
                     /locus_tag="ACHKTW_003735"
     CDS             complement(2435..2878)
                     /locus_tag="ACHKTW_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HFO6464674.1"
                     /translation="METLITISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARKAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRGSGTEQA"
     gene            2929..3231
                     /gene="yhbQ"
                     /locus_tag="ACHKTW_003736"
     CDS             2929..3231
                     /gene="yhbQ"
                     /locus_tag="ACHKTW_003736"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HFO6464675.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAESAGFAELLSSLQTPEIKSD"
     gene            complement(3218..3721)
                     /locus_tag="ACHKTW_003737"
     CDS             complement(3218..3721)
                     /locus_tag="ACHKTW_003737"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO6464676.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFQVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(3715..4239)
                     /locus_tag="ACHKTW_003738"
     CDS             complement(3715..4239)
                     /locus_tag="ACHKTW_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HFO6464677.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNAQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGLYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            4448..5443
                     /locus_tag="ACHKTW_003739"
     CDS             4448..5443
                     /locus_tag="ACHKTW_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HFO6464678.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQNFIPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            5452..6330
                     /locus_tag="ACHKTW_003740"
     CDS             5452..6330
                     /locus_tag="ACHKTW_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFO6464679.1"
                     /translation="MKYSLGPVLWYWPKETLEDFYQQAATSSADVIYLGEAVCSKRRA
                     TKVGDWLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGISN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELASMQGLVDVVRLSPQGTDTFAMLDAFRANENGAAPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            6536..7543
                     /locus_tag="ACHKTW_003741"
     CDS             6536..7543
                     /locus_tag="ACHKTW_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="luciferase-like monooxygenase"
                     /protein_id="HFO6464680.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHSPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYGV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(7642..8217)
                     /gene="dolP"
                     /locus_tag="ACHKTW_003742"
     CDS             complement(7642..8217)
                     /gene="dolP"
                     /locus_tag="ACHKTW_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006970 - response to osmotic stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/outer membrane stress-associated
                     lipid-binding lipoprotein"
                     /protein_id="HFO6464681.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKEARINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(8227..8817)
                     /gene="diaA"
                     /locus_tag="ACHKTW_003743"
     CDS             complement(8227..8817)
                     /gene="diaA"
                     /locus_tag="ACHKTW_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001483830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HFO6464682.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDV"
     gene            complement(8837..9232)
                     /locus_tag="ACHKTW_003744"
     CDS             complement(8837..9232)
                     /locus_tag="ACHKTW_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HFO6464683.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEVQARRWLEGKGLRFVAANVN
                     ERGGEIDLIMREGRTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            complement(9190..11226)
                     /gene="lpoA"
                     /locus_tag="ACHKTW_003745"
     CDS             complement(9190..11226)
                     /gene="lpoA"
                     /locus_tag="ACHKTW_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HFO6464684.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAVEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTVDGVASPAQASVSDLTGEQPAAQ
                     PAPVSTPAATTTAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSSLGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGTPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGATL
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPAS"
     gene            11290..12150
                     /gene="rsmI"
                     /locus_tag="ACHKTW_003746"
     CDS             11290..12150
                     /gene="rsmI"
                     /locus_tag="ACHKTW_003746"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFO6464685.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRALEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQKGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGICVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIIEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQG"
     gene            complement(12193..13284)
                     /locus_tag="ACHKTW_003747"
     CDS             complement(12193..13284)
                     /locus_tag="ACHKTW_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO6464686.1"
                     /translation="MKRAPLITGLLLISTSCAYASSGGCGADSTSGATNYSSVVDDVT
                     VNQTDNVTGREFTSATLSSTNWQYACTCSAGKAVKLVYMVSPVLTTTGHQAGYYKLND
                     SLDIKTTLQANDIPGLVTDQTVSVNTRFTQIKSNTVYSAATQTGVCQGDTSRYGPVNI
                     GANTTFTLYVTKPFLGSMTIPKTDIAVIKGAWVDGMGSPSTGDFHDLVKLSIQGNLTA
                     PQSCKINQGDVIKVNFGFINGQKFTTRNAMPDGFTPVDFDITYDCGDTSKIKNSLQMR
                     IDGTTGVVDQYNLVARRRSSDNAPDVGIRIENLGGGVANIPFQNGILPVDPSGHGTIN
                     MRAWPVNLVGGELETGKFQGTATITVIVR"
     gene            complement(13295..15811)
                     /locus_tag="ACHKTW_003748"
     CDS             complement(13295..15811)
                     /locus_tag="ACHKTW_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFO6464687.1"
                     /translation="MPQRHHQGHKRTPKQLALIIKRCLPMVLTGSGMLCTTANAEEYY
                     FDPIMLETTKSGMQTTDLSRFSKKYAQLPGTYQVDIWLNKKKVSQKKITFTANAEQLL
                     QPQFTVEQLRELGIKVDEIPALAEKDDDSVINSLEQIIPGTAAEFDFNHQRLNLSIPQ
                     IALYRDARGYVSPSRWDDGIPTLFTNYSFTGSDNRYRQGNRSQRQYLNMQNGANFGPW
                     RLRNYSTWTRNDQASSWNTISSYLQRDIKALKSQLLLGESATSGSIFSSYTFTGVQLA
                     SDDNMLPNSQRGFAPTVRGIANSSAIVTIRQNGYVIYQSNVPAGAFEINDLYPSSNSG
                     DLEVTIEESDGTQRRFIQPYSSLPMMQRPGHLKYSATAGRYRADANSDSKEPEFAEAT
                     AIYGLNNTFTLYGGLLGSEDYYALGIGIGGTLGALGALSMDINRADTQFDNQHSFHGY
                     QWRTQYIKDIPETNTNIAVSYYRYTNDGYFSFNEANTRNWNYNSRQKSEIQFNISQTI
                     FDGVSLYASGSQQDYWGNNEKNRNISVGVSGQQWGIGYSLNYQYSRYTDQNNDRALSL
                     NLSIPLERWLPRSRVSYQMTSQKDRPTQHEMRLDGSLLDDGRLSYSLEQSLDDDNNHN
                     SSLNASYRSPYGTFSAGYSYGNDSSQYNYGVTGGVVIHPHGVTLSQYLGNAFALIDAN
                     GASGVRIQNYPGIATDPFGYAVVPYLTTYQENRLSVDTTQLPDNVDLEQTTQFVVPNR
                     GAMVAARFNANIGYRVLVTVSDRNGKPLPFGALASNDETGQQSIVDEGGILYLSGISS
                     KSQSWTVRWGNQADQQCQFAFSTPDSEPTTSVLQGTAQCH"
     gene            complement(15841..16536)
                     /locus_tag="ACHKTW_003749"
     CDS             complement(15841..16536)
                     /locus_tag="ACHKTW_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFO6464688.1"
                     /translation="MSKRTFAVIITLLCSFCIGQALAGGIVLQRTRVIYDASRKEAAL
                     PVANKGAETPYLLQSWVDNIDGTSRAPFIITPPLFRLEAGDDSSLRIIKTADNLPENK
                     ESLFYINVRAIPAKKKSDDVNANELTLVFKTRIKMFYRPAHLKGRVNDAWTSLEFKRS
                     DHSLNIYNPTEYYVVFAGLAVDKTDLTSKIEYIAPGEHKQLPLPASGGKNVKWAAIND
                     YGGSSGTETRPLQ"
     gene            complement(16616..17200)
                     /locus_tag="ACHKTW_003750"
     CDS             complement(16616..17200)
                     /locus_tag="ACHKTW_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO6464689.1"
                     /translation="MNKVTKTAIVGLLALFAGNAAATDGEIVFDGEILKSACEINDSD
                     KKIEVALGHYNAEQFRSVGDRSPKIPFTIPLVNCPVTGWEHDNGNVEASFRLWLETRD
                     NGTVPNFPNLAKVGSFAGTAATGVGIRIDDAESGNLMPLNAMGNDNTVYQIPADSNGI
                     VNVDLIAYYVSTVEASEITPGEADAVVNVTLDYR"
BASE COUNT         4117 a         4572 c         4361 g         4251 t
ORIGIN      
        1 cgatgcaaac cgtaggtcgg ataaggcgtt tacgccgcat ccgacatgtt ttccctcaaa
       61 tcagaacaac agcgccagcc cgccgacaat cacaccagac actgccacca tcgcccctgt
      121 cagccataag gctttcgctg ggaacgcttt gcgcagcata atcagtgacg gcaaactcac
      181 cgccgggagc gtcatcaaca atgccagcgc cggagcagtt cccatacctg ccagcatcat
      241 cgtttgtaca atcggaattt ctgctgccgt gggaatgaca aacaagcatc ctgctaccgc
      301 catcgccatc acccacatca ggctgttatc gacagcacca tcggcatggg ggaataacca
      361 gacgcgagcg gcacccaaca ccagtactgc aaggatgtaa accgggatcg tactccagaa
      421 aagcgtccat agcgccctgc cccagcggct aaaaaaaccg ccctgtgctt ccggtatgtc
      481 aatttcgacc ggtgcctgcg tttgcggtgt ttcacgcacc catttttgca ccagcgtcgc
      541 aatcagcaac accatcacca gcccggccac cagacgaatc gccgcaaaac cccagccgag
      601 gacaaacccc ataaacacca gcgtcgccgg gtttaacacc ggattgccca tccagaatgc
      661 cagcgcaccg cccatcgaca cctgttggcg acgcattccc gccgcgaccg gagccgcaca
      721 gcaggtacac atcatgcccg gcagcgaaaa cagcgttccc agcagcgtgc cgcgaaagcg
      781 cgattgccca agcgtacgca acaaccagtc acgcgggatc agcacctgaa tcaacgaacc
      841 gaggatcacc cccagcactg ccgctttcca taccgcgagg aaatagatca tcgcgtaatc
      901 caacgccgcc tgccacggat tagcatccgc ctgcgcaagg atagatttac cgatactgtg
      961 ggtttcggca gcagtaaacg ctttgccgta gtaaggttgc catttcacat accagagacc
     1021 ggcaatgaca acgagaaaga aaagcgcggg tttccaccac tgaatgggcg ttgccgcctg
     1081 agatgaagac tgaccagtca tagcattccc caggaataag ttatgataat tgagcgcgtg
     1141 aatattacgc tcactatcaa ttcttgggaa taattattca gctcttttgc gtaattctga
     1201 agagcttaaa atcgtcacac cttcatgccc gggccgcatc gcctctgcca gcatgacccg
     1261 cgccacgtct ctcgcatcaa tggatttcca gttaccgggt aataaacgga acagcggcgc
     1321 aaaaagcgtt tcgttcatcc gctgtttgct acgatcgccc agtaacatcg acgggcgagc
     1381 aatggtcaat ttcggccagt tctgggcgat taatgcttct tccatctccc ctttgacgcg
     1441 gttatagaaa aacggcgagt gggcattggc acccatagca ctgaccacca gcatatgctg
     1501 cgcgcccagt cgccgcccgg ttaatgcggt atccactacc agcgtgtaat cggcatgaat
     1561 aaacgcctct ttgctccccg cttctcgccg cgtggtgccg agacaacaaa acacaatgtc
     1621 gatgggatcg gtgacctgcg ccagcgcgtc agtcagttgc ggatcatggg gattaaacac
     1681 cccgggcata tcgcccaacg gacgtcgcgt cggcgcggct atggcgttaa ctttcggttc
     1741 gttaatcaac atccgcagca ggtgaccgcc caccagcccc gttgcgcctg taatcagtac
     1801 ctgactcatc ttcgctcctt tacagaattg tctgccttgc gctccacggc tcatgcacca
     1861 ggcttaatag accatgaggt aattatcccc gattgtggga aattcgcctc atccaatgca
     1921 acaacgcgga ggaagcatga gtaagaaaat tgccgtttta atcactgatg aatttgaaga
     1981 ttcagaattt acttcacccg cagacgagtt ccgtaaagcc ggacacgaag tgattaccat
     2041 tgaaaaacaa gcgggtaaaa cggtgaaagg caaaaaagga gaagccagcg tgaccatcga
     2101 taaatccatc gatgaagtga cgccagcgga gtttgatgcc ctgctgctac cgggcggcca
     2161 ttcaccggat tatctgcgtg gcgacaaccg ttttgtcacc tttacccgtg attttgtgaa
     2221 tagcggcaaa cctgttttcg ccatctgtca cggtccgcag ctgctgatca gcgccgatgt
     2281 gattcggggg cgcaaactga ccgcggttaa accgatcatt attgatgtta aaaacgcggg
     2341 cgcggaattt tacgatcagg aagtcgtggt cgataaagat cagctggtta ccagccggac
     2401 accggacgat ctgccagcgt ttaaccgcga ggcgttacgc ctgctcggtg cctgagccgc
     2461 gtagccaaat cattttttta ccaaagccca gcgtgttgtc ggtgaatttg atttcatcga
     2521 gccggatttc ccacaccggg gccgacagca ttctggcaac cggaaagcga cgattgtacg
     2581 ccttgcgcgc gaggtcgctt tcctcacctt ccagcctgcg gatctcacct ttaaactgca
     2641 cgccgcgaat taacgctacc gttttcggct gaccgtttac cgttccggca accgcagcct
     2701 gcggcccact catctgcgcg tggcgcgttt tttcttccgt caaaatgtag aaggcgactt
     2761 tctgcgcatc aaacagataa aaagcattag cgcaccagag ttccccttcc tgctgcacac
     2821 accaggtgac aacatgttgt tttgccaacc aacggctgat ggtgatgagt gtttccattg
     2881 ctgttctctt ttatactgtg ggcctgacct taacatgcga cgcagacgat gacaccctgg
     2941 tttctttact tgatccgtac cgccgacaat aagctttata ccgggatcac cacggatgtc
     3001 gaacgccgct atcagcagca ccaaagcggc aaaggggcga aagcactgcg cgggaaagga
     3061 gaactgacgc tggcgttttc tgcgccagtg ggcgatcgtt cgctggcgtt acgggcggaa
     3121 tatcgcgtta agcaactgac caaacggcaa aaagagcgtc tggtagcgga aagcgcaggg
     3181 tttgcagagc tgttaagcag tctgcaaacc ccggagatta aaagcgattg aaatgctcgt
     3241 gatactcaac cagaccagtt acgccattca acgcgtcatc cgctaaacga tgtacctgga
     3301 aggcgctttc agtgcctggc cagcggcaac gcaggtcgtg atgtgccgcc agttcaaagc
     3361 caaaacggct gtacagcgcc ggatcgccca gcgtcaccac tgcggcatag ccgaactcat
     3421 taagcgaatc gagtccttca tagaccagtt ggcgtgccag cccctgtcca cggtattttt
     3481 catcaaccgc caacggtgcc atgccgaccc attgcaggtc ttcgccctgc acatcaaccg
     3541 gactaaatgc cacatagcca atgacctgac cttcgtcatc tgtcgccacc agccccagcg
     3601 tcagaaagcc atcttcacgc agatcgtgaa ccagcttcgc ttccgcatca ctttcgaatg
     3661 agcgacgtag cagggcatca atacccggcg catcaatggg aatttctact cgaattagca
     3721 tggttcacct accgatgtct gtttggtttc aggcgcggtt ttcattcccg cctcaacaaa
     3781 atccgccagt tgcagcagca tcatgcgtaa ggctttcggc atttgctcca gttcaatggc
     3841 gtccatcagg tttttcacat acagccccag ctccgtatcg ccttcaatca ccagccgacg
     3901 ctggaagaag agcgtatccg gatcttgttt acgcgccgca atcatcagca gatcgctggc
     3961 gtcggcacta aaactcacat cagcttgcgc gttctggctg accaccagtt tgccattcac
     4021 caccgaggta aaccattgca ggtcaatatc acgcacatga atacttaacc agcggccttc
     4081 aagaaactcc agctcgccat catccagcgc ctgacggaat tgccagctta agacctgctc
     4141 aagaacctgg cgttttagcg caaatggcgt cagttttacc ggtacactca acagagatgg
     4201 ccccaaatgc acaatacggg aacgcagttt atccaacacg agttttactc cctgtttcaa
     4261 caatcatcct attttgccat atcagaaaaa taacatagcg gtataaatca acaattccat
     4321 atgaaattgc tgctaccacc aatacaactt taactgcctt aaatcaaaaa ttgtcgcagc
     4381 aaggttaact aaaatcccag ttcgttaaca tttttgcgtt ttgatagcgc aaccttcagg
     4441 aaaaattatg gagctgctct gccctgccgg aaatctcccg gcgcttaagg cggccatcga
     4501 aaacggcgca gatgctgttt atatcgggct aaaagatgat accaatgccc gtcacttcgc
     4561 cggccttaac tttaccgaga aaaaattgca ggaagcggtg agttttgtcc atcaacatcg
     4621 ccgcaaactt cacatcgcga ttaacacttt tgcgcatccg gacggttacg cccgttggca
     4681 gcgtgccgtg gatatggcgg cgcagctggg tgccgacgcg ctgatcctcg ccgacctcgc
     4741 catgctagag tacgccgccg agcgttaccc gcatattgag cgtcatgtgt cagtgcaggc
     4801 ttcggcgacc aatgaagagg cgattaactt ttatcatcgc cattttgacg ttgctcgcgt
     4861 ggtgctgccg cgcgtgttgt cgattcatca ggtgaaacaa ctggcacggg tcacacctgt
     4921 accactggaa gtctttgctt tcggcagcct gtgcattatg tcggaaggtc gttgctatct
     4981 gtcgtcgtat ctgacgggtg agtcgcccaa caccgtaggc gcgtgttctc cggcccgttt
     5041 cgtgcgctgg caacaaacgc cgcaggggct ggaatcccgc ctgaacgaag tgctgatcga
     5101 ccgttatcag gacggcgaaa acgcaggtta tccgacgcta tgtaaagggc gttatctggt
     5161 ggacggcgag cgctatcacg cgctggaaga accaaccagt ctcaataccc tggaactgct
     5221 gccggagtta atggcggcga atattgcttc ggtgaaaatt gaaggccgcc agcgtagccc
     5281 ggcgtatgtc agccaggtgg cgaaagtctg gcgtcaggct atcgaccgtt gtaaggccga
     5341 tccgcaaaac ttcataccgc aaagcgcgtg gatggagacg ctcgggtcga tgtccgaagg
     5401 cacgcagacc acccttggcg cgtatcaccg taaatggcag tgagaagagc aatgaaatat
     5461 tccttagggc cagtgctgtg gtactggcca aaagagacgc tggaagattt ttatcagcag
     5521 gccgccacca gcagcgccga cgtgatttat cttggtgaag cggtatgcag caagcgccgg
     5581 gcaaccaaag ttggcgactg gctggagatg gcaaaatcgc tcgccggaag tggcaagcag
     5641 attgtgctct ccacgctggc gctggtgcag gcatcctctg aactgggcga actgaaacgc
     5701 tatgttgaga acggtgagtt tctgattgaa gccagcgatc tcggcgtggt gaatatgtgc
     5761 gctgaacgca aactgccgtt cgtcgccggg cacgcgctga actgctacaa cgcggtgacg
     5821 ctgaaaatat tgctcaaaca gggcatgatg cgctggtgta tgccggtgga gctttcccgc
     5881 gactggctgg tgaatctgct taatcagtgc gatgagctgg gcattagcaa ccagtttgaa
     5941 gtggaagtcc tgagctacgg tcatctaccg ctggcctact ccgcccgctg ctttaccgcg
     6001 cgttcggaag accgcccgaa agatgagtgt gaaacctgct gcattaagta tccgaacggg
     6061 cgcaacgtgc tgtcgcagga aaaccaacaa gtgtttgtac tcaatggcat tcagaccatg
     6121 agcggctacg tttacaacct cggtaacgag ctggcatcca tgcaggggct ggttgatgtg
     6181 gtccgcctgt caccgcaggg tactgacact ttcgcgatgc tcgacgcctt ccgcgctaat
     6241 gaaaatggcg ctgcgccact accgttgacg gcaaacagcg actgtaacgg ctactggcgg
     6301 cggctggccg ggctggagct gcaagcctaa gtaaatatgc tcgtcatact tcaagttgca
     6361 tgtgctgcat ctgcattcgt tcaccccagt cacttactta tgtaagctcc tggggattca
     6421 ctctcttgtc gccttcctgc aactcgaatt atttagagca cagctcactt tgttaacaac
     6481 tttaactact ctttaatgca gtattaaaga ttaatcggta acaaagtgag ctgttatgac
     6541 tgataaaacc attgcgtttt cgctactcga tctggccccc attcccgaag gttcttcagc
     6601 gcgagaagca ttctcccact ctctcgatct cgcccgtctg gctgaaaagc gcggctatca
     6661 tcgctactgg ctggcagaac accacaatat gactggcatt gccagtgctg ccacgtcggt
     6721 attgattggc tatctggcgg cgaataccac cacgctgcat ctggggtctg gcggcgtgat
     6781 gttgcctaac cactcaccgt tggtcattgc cgaacagttc ggcacgctca atacactcta
     6841 tccggggcga atcgatttgg ggctgggtcg tgcgccaggt agtgaccaac ggacaatgat
     6901 ggcgctgcgt cgccatatga gcggcgatat tgataatttc ccccgcgatg tcgcggagct
     6961 ggtggactgg tttgacgccc gcgatcccaa tccgcatgtg cgcccggtac caggctatgg
     7021 cgagaaaatc cccgtgtggt tgttaggctc cagcctttac agcgcgcaac tggcggcgca
     7081 gcttggtctg ccgttcgcgt ttgcctcaca cttcgcgccg gatatgttgt tccaggcgct
     7141 gcatctttat cgcagcaact tcaaaccgtc ggcacgactg gaaaaaccat acgcgatggt
     7201 gtgcatcaat attatcgccg ccgatagcaa ccgcgacgct gagtttctgt ttacctcaat
     7261 gcagcaagcc tttgtgaagt tgcgccgtgg cgaaaccggg caactgccgc caccgattca
     7321 aaatatggat cagttctggt cgccgtccga acagtatggt gtgcagcagg cgctgagtat
     7381 gtcgttggta ggtgataaag cgaaagtgcg tcatggcttg cagtcgatcc tgcgcgaaac
     7441 cgacgccgat gagattatgg tcaacgggca gattttcgac caccaggcgc ggctgcattc
     7501 gtttgagctg gcgatggatg ttaaggaaga gttgttggga tagtgtgtct taacgcggga
     7561 agccgtatcc gagctggcaa cgctgtccta caaggcctga taagacgcgg caagcgtcgc
     7621 atcaggcatt acaaggggct gctatttaat aaacgtaaac gctgtggtta cccgctttac
     7681 gccgctcacc cggctggcaa tatctgccgc cgctttcgct tcacgttcag tcaccagccc
     7741 catcaggaac acttcaccgt tttcggtggt cactttcacg ttggacgatt tcaccaggtc
     7801 gctggttaag agctgcgaac gcactttggt ggtgatccac gtatcgttag acgcttcgcc
     7861 cagaccaatc ggctggccct gacgaatctc gttatacact tcgttggcac cgtctacgcc
     7921 catagcaatc tgtttggcgc gagccgaaag ttcagcgttt ggtgactgcc caaccagcag
     7981 cactttgccc tgatacgccg tgacattaat acgggcttct ttcttaatct gttcgtcttt
     8041 cgacaatgcg ctgttcacgc gcacttccag ggtaccatcg tccacctggg tgccgacact
     8101 gcgtgggtca gttgcggctt tggtacccac agcagcggta cccactacgg cagcggcaac
     8161 acaaccttgc aacagcagcg cggaaataag gactgcgatt ggcgataatg ccttcatgta
     8221 ttctccttaa acatcctggt gagggaaaag cgtgttatcg atcagatcgc acaggcaatt
     8281 taccgtcagc atatgcattt cctgaatgcg agcactacga tgcgaaggaa tgcggatctc
     8341 cacatcttgt ggccctaaca aacctgcaag ttcgccgccg tcatagccgg tcaatgccac
     8401 aatagtcata tcacgcgtaa cggcagcttc taccgctttt acaatatcgc ggctgttgcc
     8461 acgggtggaa atggctaaca atacatctcc cgcatgaccc agcgcccgca cctgttttgc
     8521 atacacttca tcatgtaagc gatcgttggc aatcgccgtt aagacaacat tatcagtatt
     8581 tagtgcaatg gcaggtaagc tgggccgctc cgtttcgaaa cggttgatca tgctggcagc
     8641 aaaatgctgt gcattggcag cggaagttcc attaccacaa cagaggattt tgttgccatt
     8701 gagcagagac tgaaccagcg tcatggctgc acgggagatg gcatccggaa gcgcctctgc
     8761 cgccgcaatt tgagtttgaa tgctttcagt gaagcaagct ttaattcttt cttgcacgct
     8821 aatccttaaa ccttaattat gagtggtcat taaaggcatc cttaatccac tcaacctcat
     8881 tcccggtgaa ggctaccaca tcgaaccggc aatccacagt atcaaaactc ccattatgac
     8941 gcgcgagcca caagcgggca gtctgtaata atttgtgttg tttgctgcgg gtcacactgg
     9001 ctgccgcgcc gccataaagc gcagagcggc ggtaacgcac ctcgacaaaa acggtggtcc
     9061 ggccttcacg cattatcaga tcgatctcgc cgccacgctc gttcacgtta gcggcgacaa
     9121 atcgcagtcc tttgccttcc agccagcgac gcgcttgtac ttcccacgca tcgccggtct
     9181 gtttggtggt taactggcgg ggactacctg accttgttgg tactgtagcc atgataactt
     9241 cctgttaatc acgcaatccg ggtttgccgt caggcttccg gtattaccgt tgatttcaaa
     9301 accctgaacc tggcgcattt gtgagaaatg atttgccagc gaccaggcat cgacgcccat
     9361 cgcatacatg cgagccagtg aataatcgtt attcaccgcg ctgagtgcct gctgcattaa
     9421 cggtagatta ccgcctgcca gcatcgggat ttcgctgtac tgcaagcctt ccatctccag
     9481 tcggaaatcc gggccagcgg tcccttgcgc actgcgggag ctggcgtaca gcgttgcacc
     9541 gctctggcta ccgttacgca tggcgatcat cggtttgata aaagcgattt cacccggcgt
     9601 tgccacaatg tacaccgcat cgacacgacc gccattattg gtgaactggt catcggttgg
     9661 cgtggtttgc agcgttggat tgttggtcgt catgccggag tcggttgtcg ctctgggagt
     9721 aatcggggta cccgttaaag cgataccaga accgccgtta acacccgcgc gtaattcgct
     9781 ggtggaacca aatttttgtt gcagaacggt gccgccgccc agtttctgcc actcctgcgc
     9841 aaacgcattg gctacgcgat cgcccaatga actgcgtggg atcagcacca gcggcgcttg
     9901 tttaccctgg tcacgaatat gacgcgctgc atcgcgcgct tcgtcttccg gtgaaagcgc
     9961 gaagtaacaa atattgacgc ggttttcgat attctccggc tggttcagtg ccagcacgtt
    10021 cagcggggtg ttgctcttaa gcaactcttc aacgttattt ttcagcaacg gaccaaccac
    10081 aatactcgcg ccatcctgct gaacctggct taagatctgg ctaagtggtt gtgatgaggt
    10141 atcgtagatt ttcagttctg cggatggatt cgctggcgcg cttaccgctg tggtggttgc
    10201 tgccggggtg cttacaggcg ctggctgtgc cgcaggctgt tcaccggtca gatcgctaac
    10261 cgaggcttgt gccgggctgg caacgccatc cacggtttgc ggctgaggtt gttcagctac
    10321 gtctgcggca ggtgcggcag ctacctgagc cgccactggc tgagtgccga tatttttcgc
    10381 cgcttcaaag ccttgctgaa tagtgcgacc aaataccgct gcctggccat tcagcggcaa
    10441 cagcagggcg attttgttgg tcgaggctgg tttaaacgct tttacgttaa ccaactgcgt
    10501 tggcagcatt ttcgcgcccg gattgttcgg ataacgtttt tgccagtcgg cgatcccggc
    10561 tttcatcatg tcgggatcgt tacggttatc aaaccagacg cgctgcagat ccagccagcc
    10621 ttgcagaata ttttcgtcgg cgttgatcac cagcgtattc gcctgttcct gagtcatgga
    10681 ggagagcgcc tgccaggtgg catcaatatt ctgctgtttt tctttcgcgc cgagcagcgg
    10741 ttcctgagca attaacgcgc gcagtaaatc aatggaagga cgcccctggc tggcatcgat
    10801 ttttgcctgc cagtaacgcg cttgctggtt ttgttctaaa tcggcaggtg tgattttcgc
    10861 cagcaagttt tgcgcgccag caaaatcttt ctgcgccagt ttaatctcta ccgccagcag
    10921 tgttttctcg cgacgctgag aatcgttcag ttcttgcggt agttggttaa acaactcaac
    10981 cgcctgcccg gttttacctt ctttcaccag tgcacgaatg gcgagtaatt gccagttgat
    11041 cctggtatca tcagagcttt gctgcatctg ctgaagataa aaggcagaat cagcctgcgc
    11101 cgtgccctgc atataagcag tggactgatc gggagtatgg gtgccacaac cggcgaaaat
    11161 cagggctgcc agaacaacag gcagacaacg cgcggctttc aaacgagaaa atgttgaggg
    11221 taccataatg tatccagtga tattttttta cgcaatgctc aatattaaat cggcaatacg
    11281 gacgacacaa tgaaacaaca ccaatcggcg gataattctc agggccagct ttacattgta
    11341 ccgacgccaa tcggcaatct ggcggatatc acccagcgtg cgttagaggt attacaggcc
    11401 gttgatctga ttgccgccga ggatactcgt cacaccggtt tattgctgca acattttggg
    11461 attaatgccc ggttgtttgc gctgcacgac cataacgaac aacaaaaagc cgaaacgctg
    11521 ctggcgaagc tgcaaaaagg gcagaacatt gcgctggttt ccgatgccgg aacgccgcta
    11581 attaacgatc ctggctacca tctggtgcgt acctgccgtg aagcggggat ttgcgtggtg
    11641 ccgctacccg ggccgtgtgc tgctatcact gcgttaagcg ccgcgggttt accctctgac
    11701 cgtttctgtt acgaaggctt tttacctgcc aaatcaaaag gccgccgtga tgcgctaaaa
    11761 gccattgaag cggagccgcg cacgctgatt ttttatgaat ccacccaccg tctgttagat
    11821 agcctggaag atatcgttgc ggtattaggc gaatcccgct acgtggttct ggcgcgtgag
    11881 ctgaccaaaa cctgggaaac cattcacggc gcgcccgttg gcgagctgct ggcgtgggta
    11941 aaagaagatg aaaaccgtcg caaaggcgaa atggtgctga ttatcgaagg tcataaagca
    12001 caggaagaag acttacccgc cgatgccctg cgcacgctgg cgctgctaca ggcagaactg
    12061 ccgttgaaaa aagcggcggc gctggcggca gaaattcacg gcgtgaagaa aaatgcgctg
    12121 tataagtatg cgctggagca gcaggggtaa gagtgccgaa gcgttgcgtt aatcaaaacg
    12181 caacgtcttc ttttaccgca cgatgacggt aatggtggcg gtgccctgaa attttcctgt
    12241 ttccagctca ccaccgacca gattaactgg ccaggcgcgc atgttgatgg tgccatgccc
    12301 ggaaggatca acgggaagga taccgttctg aaaaggaata tttgcaactc cgccgccgag
    12361 attttcaata cgaataccga catcgggcgc attgtctgaa cttcttcgtc tggcgaccag
    12421 gttgtactgg tctactaccc cagttgtacc gtcgatgcgc atttgcaacg agtttttaat
    12481 ctttgaagta tcaccacagt cataagtgat atcaaagtct actggagtaa aaccgtctgg
    12541 catggcattg cgggtggtaa acttctgacc attgatgaat ccaaaattaa ccttaataac
    12601 atcgccctga ttaattttgc acgactgtgg ggcggtgaga tttccctgaa tcgataactt
    12661 gactaaatca tggaagtcac ctgtagacgg gcttcccatt ccatcgaccc atgcgccttt
    12721 aatgacggca atatccgttt tcggaatggt catcgagccg agaaatggct tggtgacata
    12781 cagggtaaag gtggtatttg caccaatatt aacgggtcca taacgagacg tgtcaccctg
    12841 gcaaacaccc gtttgggttg cagcagaata tacggtgttg ctttttatct gtgtgaatcg
    12901 ggtgttaaca gaaacggtct ggtcagtcac gagtcctgga atgtcattcg cctgtaatgt
    12961 ggttttaata tccagactat cattgagttt gtaatatcct gcttgatgtc cagtggtggt
    13021 aagtacaggg ctgaccatat agacaagttt aactgcctta cccgcagagc aggtacaggc
    13081 gtattgccag ttagtgctac ttagcgtggc agaggtaaac tcccgtcctg tcacgttatc
    13141 tgtctggttc accgtaacat catcaaccac actgctgtaa tttgtcgcac cgctagtgct
    13201 gtcagctcca caccctcctg aggaggcata agcgcaggat gtggatatca acaaaagtcc
    13261 tgttataaga ggcgctcttt tcattttttt atccttaatg gcactgcgcc gtgccttgta
    13321 atacagaggt tgttggttct gaatccggtg tactaaaagc aaactgacat tgttgatctg
    13381 cctgatttcc ccagcgtaca gtccagcttt gtgatttact cgatatccca gagagatata
    13441 gtatgccgcc ctcatcgacg atactttgtt gccccgtctc atcgttgctg gcaagagcgc
    13501 caaagggcaa cggtttaccg ttgcgatcgc tgactgtaac aagtacgcga taaccgatat
    13561 tggcgttgaa acgcgccgct accattgcac ctctgttggg caccacaaac tgtgttgttt
    13621 gctcaagatc gacgttatcg ggcagctgcg tagtatctac cgagagacgg ttttcctggt
    13681 aagtcgtgag ataaggaacc actgcatagc caaagggatc agtagcaatc cccggatagt
    13741 tttgtatcct cacgccagaa gccccattag catcgataag cgcaaaagcg ttgcccagat
    13801 attgcgagag cgtcacgcca tgaggatgga taaccacgcc gccggtaacg ccgtaattgt
    13861 attggctgct gtcattaccg tagctgtatc cggcactgaa ggtgccataa ggtgaacggt
    13921 aactggcgtt caggctactg ttatggttgt tatcgtcatc cagactttgt tccaggctat
    13981 agctcaggcg accatcatcc agcagtgagc catcaagacg catttcatgt tgggttgggc
    14041 gatctttctg gctggtcatc tgataggaaa cccggctacg cggtaaccag cgttctaacg
    14101 gaatactgag attcaaagag agtgcgcggt cattattttg atccgtgtag cgactgtatt
    14161 gataattcag gctgtaacca attccccatt gctgaccgga aaccccaaca gagatattcc
    14221 tgtttttctc gttattgccc caatagtctt gctgcgaacc ggaggcatac agacttaccc
    14281 catcaaatat tgtctggctg atgttgaatt gaatttcact tttttggcga ctgttatagt
    14341 tccaattacg ggtattggct tcattaaaac taaaatagcc atcgttggta tagcgatagt
    14401 agctgacagc gatattggtg ttggtttccg ggatatcttt gatgtactgc gtacgccatt
    14461 gatagccatg aaaagagtgc tggttatcga attgggtgtc agctctgttg atatccatcg
    14521 acaacgcgcc cagtgcgcca agtgtgccgc cgataccgat ccccagcgca taataatctt
    14581 cagaaccgag caggccgcca tacagcgtaa aagtattatt caaaccatat attgccgtgg
    14641 cttcagcaaa ttcgggttcc ttgctatcac tgtttgcatc agcgcgatag cgtccagcgg
    14701 tcgcgctata ttttagatgc ccaggtcgct gcatcatggg taatgaagaa taaggctgga
    14761 taaagcgacg ttgcgtaccg tcactttctt caatcgtgac ttctaaatcg ccgctgttgg
    14821 aagaggggta gagatcgtta atttcaaagg cacccgctgg cacgttgctt tgatagatca
    14881 cataaccatt ttgcctgata gtcacgattg cactactgtt tgcgataccg cgtaccgttg
    14941 gggcaaatcc gcgctggctg tttggcaaca tattatcgtc ggaagcgagt tgcacgccag
    15001 taaaggtgta gctggaaaaa atactgccgc tggtggcgct ttctcccaga agcaactgag
    15061 acttcaacgc cttgatatca cgttgtaaat aactactgat agtgttccag cttgacgcct
    15121 gatcgttgcg tgtccacgta gaatagttac gtaatcgcca ggggccaaaa ttagcaccat
    15181 tttgcatatt caggtactgt cgttggctac gattgccctg gcggtaacgg ttatcagaac
    15241 ctgtaaacga gtagttggta aacagcgttg gtataccatc gtcccaacga gaaggggaga
    15301 cgtaacctct tgcatcacgg tacagtgcaa tttggggaat gctcaaatta agtcgctgat
    15361 gattgaaatc aaattcagca gctgtaccgg gaatgatttg ttcaagcgag ttgatcacgc
    15421 tatcgtcatc tttttcagcc agcgccggga tttcatccac cttaataccc agctcacgta
    15481 gttgttctac cgtaaactgt ggctgcagaa gttgctctgc attggcggta aatgtaattt
    15541 ttttctgtga aaccttcttt ttattcagcc agatatcaac ctgataagtt cctggtagtt
    15601 gtgcgtattt ttttgaaaaa cgtgacagat cagttgtttg cataccactt tttgtagttt
    15661 ccagcataat ggggtcgaaa taatactctt cggcgttagc ggtagtgcaa agcatgccgc
    15721 tgccagtgag caccatcggc aaacagcgtt tgatgatgag cgccaactgt ttcggtgtgc
    15781 gtttatgtcc ctggtggtgt cgttgtggca ttccctgatg acctgtgttt tttatatttg
    15841 ttattgcagt ggacgagttt ctgtcccgga actgccgcca taatcattga ttgcagccca
    15901 tttcacgttc tttccgccag atgcaggaag tggtaactgt ttatgttctc cgggcgcgat
    15961 atattcaatt ttgcttgtga gatcggtttt atcgactgcc agtccggcaa atacgacgta
    16021 atattcagtt gggttatata tattgagtga atggtcacta cgtttaaatt ccagtgatgt
    16081 ccacgcatcg tttacccgtc ccttcaggtg tgcggggcga taaaacattt tgatccgtgt
    16141 tttaaatacc agcgtcaact cgttagcatt aacatcatct gattttttct ttgctggaat
    16201 ggcacgaaca ttaatgtaga acagcgactc tttattttca ggcaggttat cagctgtttt
    16261 aataattcgc agtgatgagt catcgccagc ttcaagacga aatagcggtg gggttataat
    16321 aaatggggca cggcttgtac catctatatt atctacccat gattgcagta aataaggcgt
    16381 ttctgcgcct ttgtttgcga caggtaacgc agcctctttg cggctggcat catagatcac
    16441 tcgcgttcgc tgtaaaacga taccccctgc aagcgcctgg ccgatacaga agctacacaa
    16501 caaggttatt atcaccgcga atgttcgttt tgacatagtt atttttcaac ctgatacaac
    16561 gagaaagagg aaggaaaagg gcgttatctg aaaggtcaga taacgccgta acgtattaac
    16621 ggtaatccag cgttacgtta acgacagcgt ctgcttcccc tggggtgatt tctgatgctt
    16681 caacggttga tacgtagtaa gcgatgaggt caacattgac aataccattg gagtccgctg
    16741 ggatctgata aaccgtgtta tcattcccca tagcattcag tggcataagg tttccgcttt
    16801 ccgcatcgtc aatacggata cccacacctg ttgccgctgt gccagcaaaa gagcccactt
    16861 ttgccagatt agggaagtta gggacggtgc cattatcccg cgtttccagc cataaacgga
    16921 aagacgcttc aacgttgccg ttatcgtgct cccacccagt cactgggcag ttaactaaag
    16981 gaatagtgaa tggaattttt gggctacgat cgccaacaga acgaaactgt tcggcattat
    17041 agtgaccaag agcaacttca attttcttat cagagtcatt gatttcacaa gcggatttta
    17101 aaatctcgcc atcaaaaact atttcaccat cggttgcagc ggcattacct gcgaaaagcg
    17161 caagtaagcc cacaatagct gtttttgtaa ctttattcat ttataacctt atccttgtaa
    17221 caagtacata atcatattta atgtgaatat atgttcaaca tctttaaaga ttatacaggt
    17281 agaaatattt cattgaacat t
//
LOCUS       DAZFQD010000113        17132 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.113, whole genome shotgun sequence.
ACCESSION   DAZFQD010000113 DAZFQD010000000
VERSION     DAZFQD010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17132
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.113"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            68..322
                     /gene="bssS"
                     /locus_tag="ACHKTW_003751"
     CDS             68..322
                     /gene="bssS"
                     /locus_tag="ACHKTW_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HFO6464690.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFPVNEYRRH"
     gene            437..1555
                     /gene="solA"
                     /locus_tag="ACHKTW_003752"
     CDS             437..1555
                     /gene="solA"
                     /locus_tag="ACHKTW_003752"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050131 - N-methyl-L-amino-acid oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HFO6464691.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETADGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVVSDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPDHDNTLFITG
                     LSGHGFKFASVLGEIAADFAQDKKSDFDLTPFRLSRFQ"
     gene            1603..1716
                     /locus_tag="ACHKTW_003753"
     CDS             1603..1716
                     /locus_tag="ACHKTW_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceO family protein"
                     /protein_id="HFO6464692.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF"
     gene            1977..2543
                     /gene="yceJ"
                     /locus_tag="ACHKTW_003754"
     CDS             1977..2543
                     /gene="yceJ"
                     /locus_tag="ACHKTW_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HFO6464693.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALYLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            2547..3122
                     /gene="yceI"
                     /locus_tag="ACHKTW_003755"
     CDS             2547..3122
                     /gene="yceI"
                     /locus_tag="ACHKTW_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HFO6464694.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTAKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(3164..4216)
                     /gene="trhO"
                     /locus_tag="ACHKTW_003756"
     CDS             complement(3164..4216)
                     /gene="trhO"
                     /locus_tag="ACHKTW_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HFO6464695.1"
                     /translation="MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDVLFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            4441..5361
                     /gene="lpxL"
                     /locus_tag="ACHKTW_003757"
     CDS             4441..5361
                     /gene="lpxL"
                     /locus_tag="ACHKTW_003757"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HFO6464696.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPVIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            5533..6759
                     /gene="mdtG"
                     /locus_tag="ACHKTW_003758"
     CDS             5533..6759
                     /gene="mdtG"
                     /locus_tag="ACHKTW_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtG"
                     /protein_id="HFO6464697.1"
                     /translation="MSPCENDTPINWKRNLIVAWLGCFLTGAAFSLVMPFLPLYVEQL
                     GVTGHSALNMWSGIVFSITFLFSAIASPFWGGLADRKGRKLMLLRSALGMGIVMVLMG
                     LAQNIWQFLILRALLGLLGGFVPNANALIATQVPRNKSGWALGTLSTGGVSGALLGPM
                     AGGLLADSYGLRPVFFITASVLILCFFVTLFCIREKFQPVSKKEMLHMREVVTSLKNP
                     KLVLSLFVTTLIIQVATGSIAPILTLYVRELAGNVSNVAFISGMIASVPGVAALLSAP
                     RLGKLGDRIGPEKILITALIFSVLLLIPMSYVQTPLQLGILRFLLGAADGALLPAVQT
                     LLVYNSSNQIAGRIFSYNQSFRDIGNVTGPLMGAAISANYGFRAVFLVTAGVVLFNAV
                     YSWNSLRRRRIPQVSN"
     gene            6842..7216
                     /gene="msyB"
                     /locus_tag="ACHKTW_003759"
     CDS             6842..7216
                     /gene="msyB"
                     /locus_tag="ACHKTW_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HFO6464698.1"
                     /translation="MTMYATLEEAIDAAREEFLADNPGIDAEDANVQQFNAQKYVLQD
                     GDIMWQVEFFADEGEEGECLPMLSGEAAQSVFDGDYDEIEIRQEWQEENTLHEWDEGE
                     FQLEPPLDTEEGRAAADEWDER"
     gene            complement(7217..7444)
                     /gene="yceK"
                     /locus_tag="ACHKTW_003760"
     CDS             complement(7217..7444)
                     /gene="yceK"
                     /locus_tag="ACHKTW_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HFO6464699.1"
                     /translation="MRLIVVSIMVTLLSGCGSIISRTIPGQGHGNQYYPGVQWDVRDS
                     AWRYVTILDLPFSLVFDTLLLPIDTHHGPYE"
     gene            complement(7617..10160)
                     /gene="mdoH"
                     /locus_tag="ACHKTW_003761"
     CDS             complement(7617..10160)
                     /gene="mdoH"
                     /locus_tag="ACHKTW_003761"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HFO6464700.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
     gene            complement(10153..11688)
                     /gene="mdoG"
                     /locus_tag="ACHKTW_003762"
     CDS             complement(10153..11688)
                     /gene="mdoG"
                     /locus_tag="ACHKTW_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HFO6464701.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPQGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKTTEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            12082..13239
                     /gene="mdoC"
                     /locus_tag="ACHKTW_003763"
     CDS             12082..13239
                     /gene="mdoC"
                     /locus_tag="ACHKTW_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HFO6464702.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            complement(13247..14728)
                     /gene="clsC"
                     /locus_tag="ACHKTW_003764"
     CDS             complement(13247..14728)
                     /gene="clsC"
                     /locus_tag="ACHKTW_003764"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HFO6464703.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRKMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRMFDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEIILKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(14670..15203)
                     /gene="ymdB"
                     /locus_tag="ACHKTW_003765"
     CDS             complement(14670..15203)
                     /gene="ymdB"
                     /locus_tag="ACHKTW_003765"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HFO6464704.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYGYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            complement(15298..15609)
                     /gene="ymdA"
                     /locus_tag="ACHKTW_003766"
     CDS             complement(15298..15609)
                     /gene="ymdA"
                     /locus_tag="ACHKTW_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmdA"
                     /protein_id="HFO6464705.1"
                     /translation="MFRPFLNSLMLGSLFFPFIAIAGSTAQGGVIHFYGQIVEPACDV
                     STQSSPVEMNCPQNGSVPGKTYSSKALMSGNVKNAQIASVKVQYLDKQKKLAVMNIEY
                     N"
     gene            complement(15730..16062)
                     /gene="csgC"
                     /locus_tag="ACHKTW_003767"
     CDS             complement(15730..16062)
                     /gene="csgC"
                     /locus_tag="ACHKTW_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly chaperone CsgC"
                     /protein_id="HFO6464706.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDVYTIIPEVTLTQSCLCRVQIL
                     SLREGSSGQSQTKQEKTLSLPANQPIALTKLSLNISPDDRVKIVVTVSDGQSLHLSQQ
                     WPPSSEKS"
     gene            complement(16122..16574)
                     /gene="csgA"
                     /locus_tag="ACHKTW_003768"
     CDS             complement(16122..16574)
                     /gene="csgA"
                     /locus_tag="ACHKTW_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli major subunit CsgA"
                     /protein_id="HFO6464707.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGNHGGGGNNSGPNSEL
                     TIYQYGGGNSALALQTDARNSDLTITQHGGGNGADVGQGSDDSSIDLTQRGFGNSATL
                     DQWNGKNSEMTVKQFGGGNGAAVDQTASNSSVNVTQVGFGNNATAHQY"
     gene            complement(16615..17070)
                     /gene="csgB"
                     /locus_tag="ACHKTW_003769"
     CDS             complement(16615..17070)
                     /gene="csgB"
                     /locus_tag="ACHKTW_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli minor subunit CsgB"
                     /protein_id="HFO6464708.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSAQLRQGGSKFLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAIVVQRQSQMAIRVTQR"
BASE COUNT         4287 a         4383 c         4074 g         4388 t
ORIGIN      
        1 ttgcttcaga taaaaacgca ttgaacctcg aataacgttg tctagtaaca cgaattaggg
       61 ggccatgatg gaaaaaaata atgaagtcat tcagactcat ccgctcgtag ggtgggacat
      121 cagcaccgtt gatagctatg atgcgctgat gttgcgtttg cactaccaga ccccaaataa
      181 gtccgagcag gaagggactg aagttggtca gacgctctgg ttaaccactg atgttgccag
      241 acaatttatt tcgatattag aagcaggaat cgccaaaatt gaatccggtg atttcccggt
      301 aaacgagtat cggcgtcatt aacatgacat gaccatccac ccaaaaggca cctcaccggt
      361 gcctttacca tttctgggtt gtatatctgc attcagttaa ttactctgct taaaccatga
      421 gagcagagag aaaaaaatga aatacgatct catcattatt ggcagcggtt ccgtaggcgc
      481 tgccgccggg tattatgcaa cccgcgccgg tttaaacgtg ctaatgaccg acgcccatat
      541 gccaccgcat caacacggca gccaccacgg cgatacgcga ttaattcgcc atgcttatgg
      601 tgaaggcgaa aagtatgtcc cactggtact ccgcgcgcaa acactgtggg atgagctctc
      661 ccgccacaac gaagatgatc ccatttttgt acgctctggt gtcattaacc tcggcccggc
      721 tgactccgca tttctcgcca acgtcgccca cagcgccgaa cagtggcaac tcaacgtcga
      781 aaagctcgac gcgcaaggga ttatggcccg ctggccagaa atacgcgtcc cggacaacta
      841 catcggctta tttgagactg attccggttt tttgcgcagc gaactggcga ttaaaacctg
      901 gatccaactg gcgaaggaag cgggctgtgc gcagctgttc aactgcccgg tcaccgcaat
      961 tcgtcatgac gatgatggcg taactattga aacggctgac ggagagtatc aggcgaaaaa
     1021 agcgattgtc tgcgcgggaa catgggtaaa agacctgctc ccggagctgc ctgtccagcc
     1081 cgtacgcaaa gtatttgcct ggtatcaggc cgatggtcgc tatagcgtga agaataaatt
     1141 cccggcgttt accggtgaac tgcccaatgg cgatcaatat tatggttttc cggcagaaaa
     1201 cgacgcgttg aagattggca aacataacgg aggccaggtt atccattcag cggatgaacg
     1261 tgttccgttt gcggaagtgg tcagcgatgg ttcggaagcc ttcccgttct tgcgcaatgt
     1321 attgccgggt atcggttgct gcctgtacgg cgctgcctgc acctatgata attcgcctga
     1381 cgaagatttt attatcgata ccctacccga ccacgataat acactgttca ttaccggcct
     1441 gagtgggcac ggttttaaat ttgcgtcagt tttaggggaa atagctgccg attttgcgca
     1501 agacaaaaaa agcgattttg atttaacgcc attcaggctt tcccgcttcc aataatcatc
     1561 attacggcct ccagaatgag gccgttttta caggagtatt taatgcgtcg gctgttgcac
     1621 tatctcatca ataatattcg cgagcatctg atgctttatc tttttctctg gggattgttg
     1681 gcaattatgg atctgatcta tgtgttttat ttttagtttt ccgcactgac ataaactgac
     1741 aaatatttaa tctccctttc atttagcgtg aaaaattgaa tgagattatt cctgcttcta
     1801 cagcactcac cagacatctc tgatgctgaa cccactaaac ttatgaagat tctttcatta
     1861 ttaattcaaa attattgaaa taaaaataaa tatcattcaa tttgttattt ttgtgttgca
     1921 attcattctc cgacacgcca ttttctttct ctcgcaaatt cgttttggat gcacttatgt
     1981 cattcacaaa tacccctgaa cgctatggtg ttatatctgc ggccttccat tggctaagcg
     2041 caattattgt ctacggcatg tttgctttag gtctgtggat ggtcacgctc agttattacg
     2101 atggctggta tcacaaagca cccgaactgc ataaaagcat cggtattttg ttaatgatgg
     2161 ggctggttat tcgcgttctg tggcgtgtca tatctccacc gccgggtccg ctgccgagtt
     2221 attcgcccat gactcgtctt gccgcaaaag ctggacatct cgcattgtac ctattgctgt
     2281 tcgccatcgg catcagcggc tatctgatct caactgccga tggtaaaccg atcagcgttt
     2341 ttggctggtt tgacgtcccc gcgacccttg ccgacgctgg cgcacaggca gactttgccg
     2401 gtgccctgca tttttggctc gcgtggagcg tcgtagtact gtccgttatg cacggattta
     2461 tggccctgaa gcatcatttc atcgataaag acgacactct gaagcgcatg ctgggaaagt
     2521 cgtcatctga ctatggagta taaaaaatga aaaaaagcct gcttggttta accttcgcgt
     2581 ccctgatgtt ctctgccggt tcagcggttg ccgccgatta caaaattgac aaagaaggtc
     2641 agcacgcctt tgttaatttc cgcatccagc accttggcta tagctggtta tacggcacct
     2701 ttaaagattt cgacggtact tttacctttg acgaaaaaaa tccggctgcc gataaagtga
     2761 atgtgacaat taacaccacc agcgtcgata ctaatcacgc cgaacgcgat aaacatcttc
     2821 gcagtgcaga tttcctcaat accgcaaaat atccacaggc aacattcacc tccaccagcg
     2881 tgaagaaaga cggtgacgaa ctggatatta ccggcgatct gacgctgaat ggcgtaacca
     2941 aacccgtcac gctggaagcg aaattaattg gtcagggcga cgacccatgg ggtggtaaac
     3001 gtgcaggctt cgaggccgaa ggcaaaatta agctcaagga cttcaatatc aagacagatt
     3061 taggtccagc ttctcaggaa gtggatctga ttatttcagt ggaaggcgta cagcagaagt
     3121 aagcgtgaaa tgttgccgga tggcacgcat ccggcaatga tatttattct gttggatcag
     3181 gaatgcccag tgttgtattc agacgtccac gagacttatt aaagatctta ttgccatttt
     3241 cacgtcctgc ccgacggcgt cgctgttctt ctggcggtaa cgcgctttct tcgcagcaaa
     3301 tctcactaca acaacctttg tatttttccg cgcatactgg acactgaata aacagcaggt
     3361 ggcagccatc atttttacag ttggtatggc tgtcgcacgg cgcgccgcac tggtggcaat
     3421 gcgcgataat ctcatccgat atacgttcgc ccatccgctc gtcaaaaaca aaatttttgc
     3481 caataaaacg caccggcaag ccctgctcgc gcgccttacg ggcgtattca ataattccgc
     3541 cctcgatatg ccagactttg ttgaatccgt tatgtttcat ccaggcactg gccttttcac
     3601 aacgaatgcc gccggtgcag tacatgacga tttttttatc tttatgtgcc tgcatcatct
     3661 cgactgcttt tggcagctgc tcacggaagg tatctgccgg aatttcgagc gcgttttcaa
     3721 agtgccccac ttcatactca tagtggttac gcatgtcgat aaatagtaca tcgggatcgt
     3781 caagcatggc gttcacttcc gccgcttgca gatactcacc aacattgctg gcatcaaagt
     3841 gaggatcgtc aataccgtcg gcaacaatgc gatcgcgtac cttcatgcgc agtacccaga
     3901 aggatttccc gtcatcatcc aacgcgatat tcaggcgtaa accctctaaa gccgggtcga
     3961 aggcatagag ctgcgcgcga aatgtttcaa cattgctcgc aggtacgctg atttgcgcgt
     4021 taatgccctc atgcgccaga tacactcgcc caaaaacatt cagcgcggta aacagctgat
     4081 ataaagcgtc acgggtcgcc ttaggatcgg cgatgtggaa atacttgtaa aacgaaatgg
     4141 tggttcgcgg ttcgctctca gccaacattt tggcttttag cgcgtcgttg gaaatgcggt
     4201 tgtgtaacac tggcatggtg tacggttcct gcgagatggg aaagtaaaaa tccgcggcat
     4261 gatatagcaa ttatcgataa ttaacatcca cacattttac gctacatttg cgcattaaaa
     4321 attatttgtt atttacaagc gcggcaattt cgcccagtct tcagccacaa attttggttg
     4381 cgggcgaaaa aatgcgacaa tacatacaat tgcccgaata ggttgaaaaa caggattgat
     4441 atgacgaatc tacccaagtt ctccaccgca ctgcttcatc cgcgttattg gttaacctgg
     4501 ttgggtattg gcgtactttg gttagtcgtg caattgccct acccggttat ctaccgcctc
     4561 ggttgtggat taggaaaact ggcgttacgt tttatgaaac gacgcgcaaa aattgtgcat
     4621 cgcaacctgg aactgtgctt cccggaaatg agcgaacaag aacgccgtaa aatggtggtg
     4681 aagaatttcg aatccgttgg catgggcctg atggaaaccg gcatggcgtg gttctggccg
     4741 gaccgccgaa tcgcccgctg gacggaagtg atcggcatgg aacacattcg tgacgtgcag
     4801 gcgcaaaaac gcggcatcct gttagttggc atccattttc tgacactgga actgggcgcg
     4861 cggcagtttg gtatgcagga accggggatt ggcgtttacc gcccgaacga taatccactg
     4921 attgactggc tacaaacctg gggccgtttg cgctcaaata aatcgatgct cgaccgcaaa
     4981 gatttaaaag gcatgattaa agccctgaaa aaaggcgaag tggtctggta cgcaccggat
     5041 catgattacg gcccgcgctc aagcgttttc gtcccgttat ttgccgttga gcaggctgcg
     5101 accacgaccg gaacctggat gctggcacgg atgtccggcg catgtctggt gcccttcgtt
     5161 ccacgccgta agccagatgg caaagggtat caattgatta tgctgccgcc agagtgttct
     5221 ccgccactgg atgatgccga aactaccgcc gcgtggatga acaaagtggt cgaaaaatgc
     5281 atcatgatgg caccagagca gtatatgtgg ttacaccgtc gctttaaaac acgcccggaa
     5341 ggcgttcctt cacgctatta aatctcccct gccggatgct tcagaatggc atccggcatt
     5401 accacagcaa atccccctga tttagcgata aaagctctct ggattgcgcc ccctggaagt
     5461 cgggcgcata attagtgtgc ttatcttttc ttcttatgtt caccgcgcct ggcgcaccaa
     5521 cagcggattg ctatgtcacc ctgtgaaaat gacaccccta taaactggaa acgaaacctg
     5581 atcgtcgcct ggctaggctg ttttcttacc ggggccgcct tcagtctggt aatgcccttc
     5641 ttacccctct acgttgagca gcttggcgtt accggtcact ccgccctgaa tatgtggtcc
     5701 ggtattgtct tcagcattac atttttattt tcggccatcg cctcaccgtt ttggggtgga
     5761 ctcgccgacc gtaaaggccg aaaactcatg ctattacgct ctgccctcgg catgggcatc
     5821 gtgatggtgt tgatgggact ggcacaaaat atctggcagt ttttgatcct acgggcgctt
     5881 cttgggttac ttggcggatt tgtccccaac gctaatgctc ttatcgccac acaagtaccg
     5941 cgtaataaaa gcggctgggc gctgggtacg ctctccacag gcggcgttag tggtgcgttg
     6001 ctcggcccaa tggctggcgg cctgctcgcc gatagctacg gcttacgtcc ggtattcttt
     6061 attaccgcca gtgtgctcat actctgcttt ttcgtcaccc tgttctgcat cagagaaaaa
     6121 ttccagccgg tcagcaaaaa agagatgctg cacatgcggg aagtggtgac atcacttaaa
     6181 aacccgaaac tggtactcag cctgtttgtc accacgttaa tcatccaggt ggcgacaggc
     6241 tcaattgccc ccattctgac gctgtatgtc cgcgaactgg cgggtaacgt cagtaacgtc
     6301 gcctttatca gtggcatgat cgcctcggtg ccaggcgtgg cggctctgct aagcgcacca
     6361 cgactcggca aacttggcga tcgaatcgga cccgaaaaga tcctgattac agcgctgatc
     6421 ttttctgtac tgctgttgat cccaatgtct tacgttcaga cgccattgca acttgggatt
     6481 ttacgttttt tgctcggtgc cgccgatggt gcactactcc ccgccgtaca gacactgttg
     6541 gtttacaact cgagcaacca gatcgccggg cgtatcttca gctataacca atcgtttcgt
     6601 gatattggca acgttaccgg accattgatg ggagcagcga tttcagcgaa ctacggtttc
     6661 agagcggtat ttctcgtcac cgctggcgta gtgttattca acgcagtcta ttcatggaac
     6721 agtctacgtc gtcgtcgaat accccaggta tcgaactgat ttttcgcctt tcatacttgc
     6781 aaaagcggag aatcagctat ccttttccct gaaacctcat caactcaaag ggagaatcgt
     6841 gatgaccatg tacgcaacgc ttgaagaagc cattgacgct gcacgcgaag aatttcttgc
     6901 agacaacccc ggcatcgacg ccgaagatgc gaatgtgcaa cagttcaatg cccaaaaata
     6961 cgttttgcag gacggcgaca tcatgtggca agttgagttt tttgccgacg aaggggaaga
     7021 aggtgaatgt ttacctatgc ttagcggtga agccgcgcaa agtgtttttg atggcgacta
     7081 tgatgagata gagatacgcc aggagtggca ggaagagaat acattacatg aatgggacga
     7141 gggggaattt cagcttgagc caccgctgga taccgaggaa ggacgcgcag cagctgatga
     7201 gtgggatgaa cgttaatcac tcatacgggc catgatgagt gtcgatcggc agcagtaaag
     7261 tatcaaaaac cagagagaat ggcagatcaa ggatcgtgac ataacgccag gcggagtcac
     7321 gcacatccca ttgcacaccg ggataatatt ggttgccatg cccctgcccc ggtatagtgc
     7381 gactaataat gctgccacag ccactcagca aggtcaccat gatgctcacc acaattaaac
     7441 gcatttgata cctctttcgt aaaaaaatac cggcataacg ccggtatttt tattggctcg
     7501 ttgtttttgc cggatgcggc gtgaacgcct tatccgaccc acaaaatctt gcaaaatcaa
     7561 taaattgcag gaacgatgta ggcctgataa gcgtagcgca tcaggcaact acgtttttat
     7621 tgcgaagccg catccggttt acgcaatgcc agtggattga gctttatccc ttcgtaataa
     7681 ctcacccatg aagaatatct ctccggggaa ttccagacgc ggaaatgcag acgggccatc
     7741 gtcaccggat cgcttagcag caccaggcga cgatcgcgat tcagcttctc tggcgtctcg
     7801 ttcagcgcct gttcaacgtg gcggtcacgg gcgatttcca gcaccttact ggcgcggtga
     7861 cgcgcggtcg ccattgcggt tgccagagcg ttaaatgacg ggttaaacac tgcgtgcata
     7921 aagccatcat caagggagcg ttgacgattc atctcaagga accgatcggt atcaaccagc
     7981 acctgcggcg gcgaatactc ttccgggatc agaaacagtt tccagcgttt ggtgcgcaga
     8041 ccaacggtgg cacggctgga aatcaccgaa acaaacggtg acaggatcaa cgagaagaca
     8101 atcggtgcca gccagaacag gaaacgcaga tccagccacg ccatcccaac agcccacact
     8161 aaccctaaca gcagctgtga gccgtggcgt ttgaacgctt caccccagga agtggagtca
     8221 tcatcacgct gcggtgaatt ccacaccact tcccagccaa ggaacgcgct gacaacgaag
     8281 accgtatgga acagcatgcg taccggagcc agcagcacgg aaaaaagcac ttccagcagc
     8341 aacgataatg taacgcgcca gaagccgccg tattctttcg ttcctttgca ccagataagc
     8401 aaaatgctca ataacttcgg caggaacaac agcaccatag tcgaagcaaa aagtgcaatc
     8461 gccagctcag gacgccactg cggccacacc gggaacaact gccgtggttg caggaagtat
     8521 tgcggttcgg tcaacgcatg tactacctgc aatgcagtag agagcgcgag gaacataaac
     8581 cacagcggag cggagagata agacatcacg cccgtcagga acaccgcacg gtgaaccggg
     8641 tgcataccct tcaccaggaa cagacggaag ttcatcaggt taccgtggca ccagcggcgg
     8701 tcacgtttta gctcatcaag caagttaggc ggtaattctt cataagaacc cgggagatcg
     8761 taagcaatcc agacccccca acctgcacgg cgcatcaacg ccgcttctac aaagtcatgt
     8821 gacaggattg aaccggcaaa ggaaccttcg cccggcagcg gagccagtgc gcagtgctcg
     8881 ataaacggtt tcacgcggat aatcgcgtta tgtccccaat agtgcgactc gccaagttgc
     8941 cagaagtgca aaccggctgt aaacagtggc ccatacacgc gggtcgcgaa ctgctgacag
     9001 cgcgcataca gcgtatccat accggacgct ttcggcgacg actgaatgat cccggcgttc
     9061 gggttggctt ccatcaggcg caccagcccg cacaaacaat caccggtcat taccgagtca
     9121 gcatccagca ccaccatgta gctgtactgg ctgccccagc gacggcagaa gtcatcgata
     9181 ttaccgcttt tacgcttcac gcggcgacgg cggcggcgat agaaaatctg accttcgcca
     9241 ccgacttcag cgataagctc catccaggct ttttgctctg cgacgcagat atccgggtta
     9301 taactgtcac taagaatgta gacatcaaag tgtttggcat tcccggtggc ttttactgat
     9361 tcccacgttg cacgcaggcc agcaaaaaca cggttcacgt cttcgttaca gataggcatg
     9421 atcaacgccg tgcgatgctc cgggtttaat ggttcatcgc caactgttga cgcagatata
     9481 ctgtatttat cgcgaccaat aagcagttgc aggaagccca ttaacgccgt ccagaatccg
     9541 gcggacaccc aacagaacag taccgcaaag aggatcagga taccggtttg cagcatataa
     9601 ggcagaagct gcataaagga aacccacaaa tcctgaccaa ccatatccat aggattaatc
     9661 agcgcccaac cctgataagg aagaatggtc ttcatatacc aggtcgcgac gacagtttgc
     9721 gcgagcgtca ggatcaacag aatgtaacgg cggatggtac cgacggtacg ccacttttgc
     9781 tcgctctcct gctcttcttt ggtcaaacga gccagatagc gcggcgtgac atcgcgtcca
     9841 cgcaggcgat cccagaaacg gcctaccggg ttggtacgcc acgggtcggg aaacatcgag
     9901 gagcgttttg cttctggcat cgccttcagc tgatcgcgcc cttcgtcgtc tttaattaac
     9961 tgtccatcag caagtgaatc tggccaggct tgttccagac gcgcctttac cgagccttgc
    10021 ggggaatcat cctcccgcgc ccaggtgcgg tgttcggcat ccagcgcctg atgaacggcg
    10081 cggatatcag tcttcggcaa tgccgctttc tcgcttgcgg cgatgggcat tgcgtcaatg
    10141 tactcagttg tcttattcat tggcaggtaa ctggtagctc caggtttcac tcaacgtctg
    10201 atcggcattc accagcgcag cacgcatttc agtggttttc ttggcatctt tcactttcac
    10261 acgcatcacc agacgccagc ctttggtaac cgggttatag cgcaccgtgc tttcaactat
    10321 ctcaccatta tcaccaatgc tggtttgcgc tgtgaccggg gtatcctctg gcagtttttt
    10381 catctctgcg ccggtaaaat cgaccacaaa ggcgatagta ccgtcaggct ggcgaatcag
    10441 gttcgactgc ttcacatccc ccgttgaacg acgcgtttgt tgcacccatg cgttatctgg
    10501 cgcatgcagt ttgtcttcat cacggctgaa ggtgatggtg tatttaaagt tcatctcttt
    10561 acccggctcc ggcagctgat ccggcgtcca gtaagcgacg atgttatcgt tggtttcatc
    10621 gttggttgga atttccacca gctcaacgct gcctttaccc cactcccctt tcggagtcac
    10681 ccatgcgctt ggacgaagat cgtaacgatc atcgagatct tcaaagcggg agaaatcacg
    10741 accgcgctgc aacagaccga agccttgcgg gttctccatc gagaagctgc tgaccgctaa
    10801 atgtttcggg ttattcaacg gacgccagat ccattcgcca ttaccggcat ggatagagag
    10861 accgttagag tcgtgcaact ccggacgata gttatttgca ggcgacggtt ggttcggccc
    10921 aaacaggaac atactggtta acggtgcaac ccccagtttg ccgactttat cgcgcagata
    10981 gattttcgac tgcacatcca caaccgtgtc acgccctggc ataactacga atttgtaagc
    11041 acctgtcgcg cgcggcgagt caagcaatgc ataaatggtt aaacgtttat cagtcggttt
    11101 tggacgctcg atccagaact ctttgaagcg tggaaattct tcacccgatg gcaaggcggt
    11161 atcaattgcc aggccgcggg cagaaaggcc ataaacctga cctgcaccaa tcacgcggaa
    11221 atagctggcc ccgagcatgc tgacgatttc atcgttttta tctttgctgt tgatcgggta
    11281 aagcacttta aaaccggcaa aaccaaggtc ttttaccgtg tctttgtcat gctgaacatc
    11341 gccgaaagtg aaataatccg ggctgtattt gattcgtttg actgcggtgg cagtcacttc
    11401 atttattttg accggggtat cgaagtacat accctgatgg tagaactcga gtttgaatgg
    11461 ggtcttcaga ttgttccagt acgctttgtc atgattaaac tggatctgct gatagtccgc
    11521 gtatttcata tcgcggaaaa cggagggcaa gttgcttttg ggcgcctcat agcctttccc
    11581 ggctaaggat tgagcttgct ttgcgacatc atcaatactg aaagcccagc ttgaagatgt
    11641 atacagggtt aacattactg cagcactcaa ccaacgcatt ttcatcattt gtagtttatg
    11701 tttcataatt agtaagcact tccccctttg tgtgcttata tcgatccgat ctattttaat
    11761 ggataaccag ccattccgac aacagaatcc cggtatcgtt cagcacgctg gctcatggat
    11821 taagcacctg attgcaaccg ttttcacttt ccgtgcttct cctaaagaca gaagcatcga
    11881 cttcgtgtag ggtttgccag caattttatt tatcaaccgt cttacagacg cttccattct
    11941 ggttagtggt agcgatgatg ctatgcataa aaaccttgaa gcctgcgcct ggcgcggtca
    12001 ggtcggtgtc gctatggcaa caagcctaaa ggcataaccc gacagggata agacgaaaaa
    12061 tccgagatta gttaaccata tatgaatcca gtacccgcgc aacgtgaata tttcctcgac
    12121 tccatccgcg cctggctgat gttgttaggg atcccttttc atatttcttt aatctattcg
    12181 agccatacat ggcatgtgaa tagcgccgaa ccatcattgt ggctgaccct ttttaatgac
    12241 ttcatccact cgttccgcat gcaggtattt ttcgttatat ccggctactt ttcctacatg
    12301 ctttttttac gctatccctt gaaaaaatgg tggaaagtac gtgtcgaacg tgtaggtatc
    12361 ccgatgttaa cagccatccc cctactgaca ttaccgcaat ttattatgct gcaatatgtc
    12421 aaaggaaaag cggaaagttg gcctgggctg tcattgtatg acaaatataa tacgttggcc
    12481 tgggaattaa tatcacacct gtggttttta ctggtgttag tagtcatgac gacgctgtgc
    12541 gtatggatat ttaagcgcat cagaaataat ttagaaaatt ctgataaaac gaataaaaaa
    12601 ttctcgatgg taaaactatc ggtgattttt ttatgcctcg gcatcggtta tgcggtaata
    12661 agaagaacga tttttattgt gtatccgccc attctgagta atggcatgtt caattttatt
    12721 gtcatgcaaa cgctgtttta tttgccgttc tttatcctcg gcgcactggc tttcattttc
    12781 cctcatctta aagccttgtt taccacgccg tctcgtggct gtacccttgc agcagcattg
    12841 gcgtttgtcg cttatttact caaccagcgc tatggcagtg gcgatgcctg gatgtacgaa
    12901 accgagtcgg tgatcaccat ggtcctcggt ctgtggatgg tgaatgtggt cttctccttt
    12961 ggccaccgtt tgcttaactt ccagtcagcg cgggtgactt attttgttaa cgcatcgctg
    13021 tttatctatc tggttcacca cccgttaacg ctgtttttcg gcgcatacat tacaccgcac
    13081 atcacctcca actggcttgg ttttctctgt ggcctgatat ttgtagtagg gattgcgata
    13141 attctgtatg aaattcattt gcgcatcccg ttactgaagt ttttgttctc tggtaaaccg
    13201 gttgttaagc gtgagaacga taaagcacca gcccgttaag ccacatttac aataaccatt
    13261 ccacgggcaa tatcgacgcc agtctgacca taacccgctt ccagaaactg gtggcgggtt
    13321 cttttttgag aataatctct ttctttgcat gacgatcaac ccagttgatc cgtccccacc
    13381 tgtccagacg gagctgccag gccgcatcat actggctctg aataaagcgt ttatcaatta
    13441 actgtgccag cgtttcgctc tctatcacga agcccatttc agtattgagc aatgttgaac
    13501 gcggatcgaa attgaaagag ccgataaaca ccgttttacc atcgatgcta aaggttttag
    13561 catgcaggct ggctccggaa ttaccggtta tgccgcgatc gtgtaacgta ctactttgtt
    13621 cacgcgtcgg cttgagttca tataattcca cgccatagcg gagcaatttt ttgcgccagc
    13681 gcgcgtatcc ggcatggacg acagcaacat cgttagcggc aagagaattg gttaggatcg
    13741 caatctttac cccttttctc accatccgta agagttgcgc cacacctgcg cgtgtcggta
    13801 caaaatagga agagataata tcgatgcgtt cactgggtga gcccatgata tcgaacatgc
    13861 gctgcggtaa cagtgaatgc cgttttgcct tgccctcccc tttcgccgga tcatcactta
    13921 ataaacgtgt tttcgcccag ataagcggca acgttccatc aaccagatga tttataaacg
    13981 gactggattc cattttgcgt aagtaacgat gcgtcatagc atcgttatgc caggaggcgg
    14041 gtaactcgat gcgatccgcc atttcacctt ccgggacatc cagcacttgc tgtaagggtg
    14101 aaaccgattt gcaataccag tagcgggcga aatcatcggc aacgtcctct accacgggcc
    14161 ctattgccat aacatctaaa tccgaaaaaa gtggctcttc ccctgcccca aaataggcat
    14221 caccaatatt tcgcccccct accagcgtca ccacgccatc gacagtgaaa cttttattgt
    14281 gcatacggcg attgagacgg gaaaagtcgg tgatataacc aagcggacgc agcaagcgaa
    14341 acgagaaagg attaaaaagc cggacttcaa tgcggggatg gctgtcaagc aagcgtaaaa
    14401 tgtcgtcaag tccgggcgta ttgttgtcgt ccagcaacaa acggacgcga acgccacgct
    14461 ttgctgcggc taacagggcg gaaaacagta accgacccga catatcatcc tgccagatgt
    14521 aatactgaac atccagcgta tgctctgcca tttcggccag acgataccgg gcggcaaacg
    14581 catccagact tttctccaga gggaaaagac cacactgacc gggatgttgc gaacacagtg
    14641 gcagcaccgc gctcgccagc cggggcaaat cattcatctc cttgttgggt aaggagtctt
    14701 tcgtagaggt gggcgttttc ttcatcataa cagacaaagt atacctgttc gggtaaagcg
    14761 tgacgggtaa taaattctga aacggtttta actgcgattt cagccgctgc cgcacgaggg
    14821 taaccataaa ccccagtact gattgcagga aaagccactg acgtatagct gtttgccgcc
    14881 accagtcgca ggctattcag ataggcatcc tgcaaaagct ggtcttcgtt ttgttcacca
    14941 ccacgccaga ctggcccgac ggtgtgtact acggctttag cgggaagatc gcctgcaagc
    15001 gtaataacgg catgccccgt agggcaatcg ccctgctgtt gcctgacttt taaacaggca
    15061 tccagcaggg ccggacccgc tgcgcgatga atggccccat cgacgccgcc gcctcccatt
    15121 aatgacggat tagccgcatt cacaatcaca tcaacggcca gtttggtaat atcaccctgc
    15181 acaacatgaa tacgcgtttt cataacgcct ccttacacct tcttgtttct taagtgtaca
    15241 gcaggctttt tggttaaatc gcatttttct gctcagcaac gtgcgttgtt cagaaactta
    15301 gttatattcg atgttcatta cagccagctt tttctgttta tcaagatact gcacttttac
    15361 tgaggctatt tgcgcgtttt taacattacc gctcattaac gctttgctgg aataggtttt
    15421 gcccggaaca gaaccattct gtgggcagtt catttctacg ggtgatgact gggtgctgac
    15481 gtcacatgcc ggttccacaa tttggccata aaaatggatc accccccctt gcgcggtgct
    15541 tcctgcgatg gcaataaaag gaaaaaacaa actgccgagc ataagagagt ttaaaaaagg
    15601 tctgaacatt tattctattt cctcaatgat tagtcatcct tgagggttgt gttatccata
    15661 ctttcaggtt ggctgattcg cagcagacca ttctctccag attcatctta tgctcgatat
    15721 ttcaacaaat taagattttt ctgaagaggg cggccattgt tgtgataaat gaagtgactg
    15781 tccatcagaa acagtaacaa ctattttcac ccgatcgtcc ggggaaatat ttaaactcaa
    15841 cttcgtcaaa gcaatgggtt gattagcagg caatgaaagg gtcttttctt gcttcgtctg
    15901 actttgccct gaactgcctt cgcgcaggga caatatttgt actctgcaca gacaagattg
    15961 agtaagagtg acttcaggaa taatggtata cacatcccct tgctgggtcg tattaaaggt
    16021 tatctggctg gaaagtgccg caaggagtaa taacgcattc atattcttct cccgaaaaaa
    16081 aaacagggct tgcgccctgt ttctgtaata caaatgatgt attagtactg atgagcggtc
    16141 gcgttgttac caaagccaac ctgagtcacg ttgacggagg agttagatgc agtctggtca
    16201 actgcagcac cgttgccacc accgaactgt ttaaccgtca tttcagaatt tttgccgttc
    16261 cactgatcaa gagtagcgct gttaccgaag ccacgttggg tcagatcgat tgagctgtca
    16321 tccgagccct gaccaacatc tgcaccatta ccgccgccat gctgggtaat agtcaagtca
    16381 gagttacggg catcagtttg cagagcaagt gcagagttac cgccaccgta ctggtaaatg
    16441 gtcagctctg aatttgggcc gctattatta ccgccaccac cgtggttacc gccgccgtac
    16501 tgaggaacaa cacctgccag agcgctaccg gagaatacga ttgctgcaat tgctgctact
    16561 tttaaaagtt tcatgtaaaa cccccatcgg attgatttaa aagtcgaatg gaaattaacg
    16621 ttgtgtcacg cgaatagcca tttgcgactg tctctgcact acaattgccg ttttttgagt
    16681 accatactgt gtaatatttg ctttattacc agaacctttc tggataatca tcgcagtatt
    16741 accataagca ccttgcgaaa tactggcatc gttggcactg cccgcctgat caatatatgc
    16801 aaggttataa tctcctgtct ggtcaatctt tgcccgattg ctactacctt cttgcgcaac
    16861 aaccgccaaa aattttgagc ctccctgccg taactgagca ctattattcg tcccagcttg
    16921 accaattatg gctgcctgat taaatgaaga cttactcaat tcatttaccg cgaagttata
    16981 ttctgaatta gctaaatcat aacctgctgc ggctgcaatc ccaggcgcac ccagtattgt
    17041 taacatcata aataacaatt tgtttttcat gttgtcaccc tggacctggt cgtacattta
    17101 agaaattaaa tcatttcaac ttggttgtta ac
//
LOCUS       DAZFQD010000114        16797 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.114, whole genome shotgun sequence.
ACCESSION   DAZFQD010000114 DAZFQD010000000
VERSION     DAZFQD010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16797
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.114"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            485..994
                     /locus_tag="ACHKTW_003770"
     CDS             485..994
                     /locus_tag="ACHKTW_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFO6464709.1"
                     /translation="MATQTEVARHLSLTDRQLRRLQKLPGAPISNKRGQLDLDAWRDF
                     YISYLRRSKNDVPDGDSEDDYEEKLLIARWELTAEQAVTQQLKNEVSKGKLIDTGFCI
                     FALSKLAMALSSTLDSIPLSMQRQFPDLTPRHLDHLKTLIAKGANQCARAGDKLPDLL
                     DEYIRATTE"
     gene            966..2894
                     /locus_tag="ACHKTW_003771"
                     /pseudo
     CDS             966..2894
                     /locus_tag="ACHKTW_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310762.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
     gene            2878..3084
                     /locus_tag="ACHKTW_003772"
     CDS             2878..3084
                     /locus_tag="ACHKTW_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gpW family head-tail joining protein"
                     /protein_id="HFO6464710.1"
                     /translation="MVTVAELQALRQARLDLLTGKRVVSVQKDGRRIEYTAASLDELN
                     RAINDAELVLGTTRRRRRPLGVRL"
     gene            3081..4673
                     /locus_tag="ACHKTW_003773"
     CDS             3081..4673
                     /locus_tag="ACHKTW_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO6464711.1"
                     /translation="MKRKPVLIDVNGVPLRESLSYNGGGAGFGGQMAEWLPPSQSADA
                     ALLPALRLGNARADDLVRNNGIAANAVALHKDHIVGHMFLISYRPNWRWLGMRETAAK
                     SFVDEVEAAWSEYAEGMFGEIDVEGKRTFTEFIREGVGVHAFNGEIFVQPVWDTESTQ
                     LFRTRFKAVSPKRVDTPGHGIGNRFLRAGVEVDRYGRAVAYHICEDDFPRSGSGRWER
                     IPRELPTGRPAMLHIFEPVEDGQTRGANQFYSVMERLKMLDSLQATQLQSAIVKAMYA
                     ATIESELDTEKAFEYIAGAPQGQKDNPLINILDKFSTWYDTNSVTLGGVKIPHLFPGD
                     DLKLQTAQDSDNGFSALEQALLRYIAAGLGVSYEQLSRDYSKVSYSSARASANESWRY
                     FMGRRKFIAARLATQMFSCWLEEALLRGIIRPPRARFDFYQARSAWSRAEWIGAGRMA
                     IDGLKEVQESVMRIDSGLSTYEKELALMGEDYQDIFRQQVRESAEREKAGLSRPVWIA
                     QAYQQQIAESRRPEEETTPRET"
     gene            4663..6168
                     /locus_tag="ACHKTW_003774"
     CDS             4663..6168
                     /locus_tag="ACHKTW_003774"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFO6464712.1"
                     /translation="MRRNLSHIIAAAFNEPLLLEPAYARVFFCALGREMGAASLSVPQ
                     QQVQLDAPGMLAETDEYMAGGKRPARVYRVVNGIAVLPVTGTLVHRLGGMRPFSGMTG
                     YDGIVACLQQAMADSQVRGVLLDIDSPGGQAAGAFDCADMIYRLRQQKPVWALCNDTA
                     CSAAMLLASACSRRLVTQTSRIGSIGVMMSHVSYAGHLAQAGVDITLIYSGAHKVDGN
                     QFEALPEEVRQDMQQRIDAARRMFAEKVAMYTGLSVDAVTGTEAAVFEGQSGIEAGLA
                     DELINASDAISVMAAALNTHDTGGTMPQLTATEAAAQENQRVMGILACQEAKGREHLA
                     TMLAGQQGMSVEQARAILTAAAPQQPVASTQSEADRIMACEEAKGREQLAATLAAMPD
                     MTVEKARPILAASPQADAGPSLRDQIMALDEAKGAEAQAEKLAAFPGMTVEAARDILS
                     SSPDKAEPVSASTTAMFERIMANHSPAAVQGGVSQTSADGDADVKMLMAMP"
     gene            6205..6552
                     /locus_tag="ACHKTW_003775"
     CDS             6205..6552
                     /locus_tag="ACHKTW_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HFO6464713.1"
                     /translation="MVTKTITEQRAEVRIFAGNDPAHTATGSSGISQATPALTPLMLD
                     EASGKLVVWDGQKAGSAAGILVLPLEGTETVLTYYKSGTFATEAIRWPDSVDEHKKAN
                     AFAGTALSHAALP"
     gene            6610..7638
                     /locus_tag="ACHKTW_003776"
     CDS             6610..7638
                     /locus_tag="ACHKTW_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFO6464714.1"
                     /translation="MGLFTTRQLLGYTEQKVKFRALFLELFFRRTVNFHTEEVMLDKI
                     TGKTPVAAYVSPVVEGKVLRHRGGETRVLRPGYVKPKHEFNYQQAVERLPGEDPAQLN
                     DPAYRRLRIITDNLKQEEHAIVQVEEMQAVNAVLYGKYTMEGDQFDTVEVDFGRSEGN
                     NIEQADGKKWSEQDRDTFDPTHDIDLYCDQASGLVNIAIMDGTVWRLLNGFKLFREKL
                     DTRRGSNSQLETAVKDLGAVVSFKGYYGDLAIVVAKTSYVAEDGTEKRYLPEGTLVLG
                     NTAAEGIRCYGAIQDAQALSEGVVASSRYPKHWLTVGDPAREFTMTQSAPLMVLPDPD
                     EFVVVQVK"
     gene            7690..8073
                     /gene="gpFI"
                     /locus_tag="ACHKTW_003777"
     CDS             7690..8073
                     /gene="gpFI"
                     /locus_tag="ACHKTW_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HFO6464715.1"
                     /translation="MATKEENLNRLRQLAGLLGREADMSGSAADIAQRVSEWEEELAV
                     SPEGIMHSDESGADQNHTDDGEQLNNTDAPDDVKAVRVRKCLQVMGYCPETGRPVELA
                     LRGMRVLVPSSLATAMIQHGTAEYA"
     gene            8066..8419
                     /locus_tag="ACHKTW_003778"
     CDS             8066..8419
                     /locus_tag="ACHKTW_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFO6464716.1"
                     /translation="MRDFQNAFDAALAGVDSTIVEVMGIRAQFTSGAQRGGEVQGVFD
                     DPESLGFAGGGVRIEGSSPSLFVRTDTVRAVRRGDTLTINGEMFWVDRVSPDDGGSCY
                     LWLNRGQPPAVNRRR"
     gene            8435..8968
                     /locus_tag="ACHKTW_003779"
     CDS             8435..8968
                     /locus_tag="ACHKTW_003779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO6464717.1"
                     /translation="MAIKGLDQAIDNLSRVRKNAIPAASAMAINRVATTAINQSSSQV
                     ARETKVRRKLVKERSRLKRATVRNPNARIIVNRGDLPVIKLGIRMLGRRPNSILKAGQ
                     HRYQRAFIQRLKNGRWHVMQRVAGKNRYPIDVVKIPMAAPLKQAFDENVDRIRRERLP
                     KELAYALKQQLRIAIKR"
     gene            8965..9360
                     /locus_tag="ACHKTW_003780"
     CDS             8965..9360
                     /locus_tag="ACHKTW_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFO6464718.1"
                     /translation="MKHTDIRAAVLDALEQHEHGATLFDGRPVVFDEEDFPAIAVYLT
                     DAEYTGEELDADTWRATLHIEVFLPAQVPDSELDQWMESRIYPAMTAIPALAGLITTM
                     VTQGYEYRRDDDMALWSSADLTYSITYEM"
     gene            9368..10120
                     /locus_tag="ACHKTW_003781"
     CDS             9368..10120
                     /locus_tag="ACHKTW_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO6464719.1"
                     /translation="MATPNPLEPVKGAGTTLWVYNGKGDAYANPLSDDDWQRLAKVKD
                     LTPGEMTAEPYDDNYLDDEDADWTATGQGQKSAGDTSFTLAWKPGEDGQKGLIGWFES
                     GDVRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGKPSVAEERSKITP
                     VTAIKVTQTSGTVEKGKTTTLTVSFEPESATDKTFRAVSADPSTGSIAVKDMVITVTG
                     IKAGKVSIPVISGNGQFATVAEVTVTEAGAAG"
     gene            10134..10565
                     /locus_tag="ACHKTW_003782"
     CDS             10134..10565
                     /locus_tag="ACHKTW_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFO6464720.1"
                     /translation="MFLKTEQFEYNGVSVMLSELSALQRIEHLALLKRRAEEAEASGN
                     LQVSVEDLVRTGAFLVAMSLWHNHPQKTQSPSMNEAVMKIEQEVLTTWPADAIARAEE
                     VVLRLSGMSGAVHADTDSTEVAKNNALTDDDFSAGKFSTAS"
     gene            10592..11005
                     /locus_tag="ACHKTW_003783"
     CDS             10592..11005
                     /locus_tag="ACHKTW_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFO6464721.1"
                     /translation="MGRPDWRAMLAGMTSTEYADWRHFYRTHYFQDTQLDMHFSGLMY
                     AVLSLFFCDPDMHPSDFSLLAPRREEAQTEMPDEEDMLMQKAAGLAGGVRFGGEGGGD
                     ISPSADVVDVSEDDVALMMASAGISGGVRYVPAGW"
     gene            10986..13565
                     /locus_tag="ACHKTW_003784"
     CDS             10986..13565
                     /locus_tag="ACHKTW_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO6464722.1"
                     /translation="MSQPVGDLVIDLSLDAVRFDEQMSRVRRHFSGLDTDARKTAGAV
                     EQSLNRQALAAQKAGISVGQYKAAMRTLPAQFTDIATQLAGGQNPWLILLQQGGQVKD
                     SFGGMIPMFRGLAGAITLPMVGVTSLAVATGALAYAWYQGDSTLSEFNKTLVLSGNQA
                     GLTADRMLTLSRAGQAAGLTFNQARESLAALVNAGVRGGEQFDAINQSVARFASASGV
                     EVDKVAEAFGKLTTDPTSGLMAMARQFRNVTAEQIAYVAQLQRSGDEAGALQAANDIA
                     TKGFDEQTRRLKENMGTLETWADKTGKAFKSMWDAILDIGRPESSADMLASAQKAFDE
                     ADKKWQWYQSRSQRRGKTASFRANLQGAWDDRENARLGLAAATLQSDMEKAGELAARD
                     RAEREASQLKYTGEAQKAYERLLSPLEKYTARQEELNKALKDGKILQADYNTLMAAAK
                     KDYESTLKKPKSSGVKVSAGERQEDRAHAALLALETELRTLEKHAGANEKISQQRRDL
                     WKAENQYVVLKEAATKRQLSEQEKSLLAHEKETLEYKRQLAELGDKVEHQKRLNELAQ
                     QAARFEQQQSAKQAAISAKARGLTDRQAQRESEEQRLRDVYGDNPAALAKATSALKNT
                     WSAEELLRGSWMAGMKSGWGEWAESATDSFSQVKSAATQTFDGIAQNMAAMLTGAEAD
                     WRGFTRSVLSMMTEILLKQAMVGIVGRIGSAIGGAFGGGASASTGTAIQAAAANFHFA
                     TGGFTGTGGKYEPAGIVHRGEFVFTKEATSRIGVGNLYRLMRGYAEGGYVGGAGSPAQ
                     MRRAEGINFNQNNHVVIQNDGINGQAGPQLMKAVYDMARKGAQDELRLQLRDGGMLSG
                     SGR"
     gene            13562..13891
                     /locus_tag="ACHKTW_003785"
     CDS             13562..13891
                     /locus_tag="ACHKTW_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO6464723.1"
                     /translation="MKTFRWKVKPDMEVNSQPSVREVRFGDGYSQRMAAGLNADLKTY
                     RVMLSVSREEARHLEAFLAEHGGWKAFLWKPPYAYRQIKVTCAGWSARVGMLRVEFSA
                     EFKQVVN"
     gene            13891..14589
                     /locus_tag="ACHKTW_003786"
     CDS             13891..14589
                     /locus_tag="ACHKTW_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO6464724.1"
                     /translation="MQDIHEASLNESVKSEQSPRVVLWEIDLTVQGGERYFFCNELNE
                     KGEPVTWQGRKYEAYPIEGSGFEMNGKGSSARPSLTVSNLFGLVTGMAEDLQSLVGAT
                     VVRRRVYARFLDAVNFVAGNPEADPEQELTDRWVVEQMSALTAMTASFVLATPTETDG
                     ALFPGRIMLANTCMWTYRSDECGYTGGAVADEFDKPTTDIRKDRCSKCMRGCELRRNV
                     GNFGGFLSINKLSQ"
     gene            14600..15343
                     /locus_tag="ACHKTW_003787"
     CDS             14600..15343
                     /locus_tag="ACHKTW_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFO6464725.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVISTPEGERYIPCVNISAEPE
                     AYFRIAPEDWLRAEMQGEIVALVHSHPGGLPWLSEADRRLQIKSALPWWLVCRGDIHK
                     FRCVPHLTGRRFAHGVTDCYTLFRDAYHLAGTEMPDFHREDEWWRNGQNLYLDNMEAT
                     GFYRVPLSSAQAGDILLCCFGASVANHAAIYCGNGELLHHLPEQLSKRERYSEKWQRR
                     THSVWRHRHWHASAFTGIYNDLAAASACM"
     gene            15340..15921
                     /locus_tag="ACHKTW_003788"
     CDS             15340..15921
                     /locus_tag="ACHKTW_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310263.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFO6464726.1"
                     /translation="MNTAAEAIRALSMQVPGFRRQMNEGWYQIRIAGEDTAPEAVYAR
                     LHEQLGEGTVIHIVPRLAGAGKGGLQIVLGAAAIVGSFFTAGATMALWGSALAAGGFS
                     ATTMLFSLGASMILGGVAQMLAPKAKTPDYRATDNGRQNTYFSSLDNMIAQGNPMPVP
                     YGEMLVGSRRISQDISTRDEGGDGKVVVIGRQG"
     gene            16175..>16797
                     /locus_tag="ACHKTW_003789"
     CDS             16175..>16797
                     /locus_tag="ACHKTW_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO6464727.1"
                     /translation="MGKGGGKAHTPREAKDNLKSTQMMSVIDAIGEGPVEGPVKGLQS
                     ILVNKTPLTDTDGNPVIHGVTAVWRAGEQEQTPPEGFESSGAETALGVEVTKAKPVTR
                     TITSANIDRLRVTFGVQSLVETTSKGDRNPSSVRLLIQLERNGNWVTEKDITINGKTT
                     SQYLTSVILDNLPPRPFNIRMVRETADSTTDQLQNKTLWSSYTEIIDV"
BASE COUNT         3852 a         4043 c         5352 g         3550 t
ORIGIN      
        1 ttgcgttacc cggatttgat aaaggcattt cagcctgaca tagccatgcg ccgtatcgtc
       61 gccgtattcc tgcattaaca gagaccgcag cccgacaggg agactcctct gcgcgagtgt
      121 gcggggataa tcaaaaacga tgcacaccgg ggtttaccgc gttaacggag cgcggcgttg
      181 tcccctcata gtcgctcgtc cggtgcgatg gtggaagaaa ctggattttg ttgcaactga
      241 taaccattat cattttctcg ggtcctttcc ggcatatgga cccgttacgg ggcggcgacc
      301 tcgcgggttt tcgctattta tgaaaatttt ccgggatcca tgtccggttt ctctgcaagt
      361 taaccatatg aaaaatataa aaacatgctt tccatgaacc ggacatgcgc aaaaaataga
      421 cactaaaacc ggacatgacc ggttttgttg tgattgtgag gtgagagttt ttgcgaggtg
      481 aggagtggct acgcagactg aagttgccag gcatttaagt ctgaccgatc gccagcttcg
      541 cagattgcag aaattgccgg gtgccccgat atcgaataag cgagggcaac tggatctgga
      601 tgcctggcgc gatttttaca tatcgtatct gagaagaagt aaaaacgatg tgcctgatgg
      661 cgatagcgaa gacgactatg aggagaaatt gcttattgcc agatgggaac tgacagcaga
      721 acaggctgtt acacagcagt taaaaaatga ggtgtcaaaa ggaaaactta ttgacaccgg
      781 gttctgtatt tttgccctca gcaagctggc aatggcgtta tccagtacgc ttgattccat
      841 ccctttatcc atgcagcgac agtttcctga tttaacaccg cgccatcttg accatctgaa
      901 aacccttatt gcgaaggggg caaatcagtg tgcgcgggcg ggggataaat taccggattt
      961 actcgatgaa tatatcagag caacaactga ataatatgat gagtgctgtc acaacagcat
     1021 tacagcccct gataagggca ttgccggtga cgccagttga atgggctgat caaaattatt
     1081 atctgcctaa agaatcttca tatggtgagg gagaatggaa aacgctgccg ttccagatcg
     1141 ccatcatgaa cagcatgggg aatgatcaga tccggactgt taatctgatt aaatctgccc
     1201 gtgttggcta tacaaagatg ttgctgggag tcgccgggta ttttattgag cataaatccc
     1261 gaaacagtct gctttttcag cccacggatt ctgccgctga agattttatg aagtctcacg
     1321 tggaggcgac gattcgcgat gttccctgcc tgaaaaaact ttctccctgg ctgggacgta
     1381 aacatcgtga taataccatc acgctgaaac gcttttcatc gggtgtgggc ttctggtgcc
     1441 tgggcggcgc tgccgccaaa aactaccgtg aaaaatccgt ggacgtggtc tgctatgacg
     1501 aactttcctc gttcgagccg gatgtcgaaa aagagggctc gccaaccctg ctgggggata
     1561 agcgtattga gggctcggta tggccaaaat ccattcgcgg ctcgacgcct aaaataaaag
     1621 gctcctgcca gatcgaaaaa gcggccaacg agtcggcaca tttcatgcgt ttttatgtgc
     1681 cctgcccgca ctgcggggag gagcagtatc tgaaatttgg cgatgagtcc acgccttttg
     1741 gccttaaatg ggagaaggac agtcccgaaa gtgttttcta cctctgtgaa catcatggct
     1801 gcgtgatcca tcagtctgaa ctggaccaga gcaacgggcg gtggatctgt gaaaacacgg
     1861 ggatgtggac ccgcgacggt ctgacgtttt tcagcgccgc ggataatgaa attccgccgc
     1921 cgcgctccat cacattccat atctggacgg cgtacagtcc gttcaccacc tgggtacaga
     1981 ttgtctatga ctggctggat gcactgaaag atcccaacgg cctgaaaacc tttgtgaaca
     2041 ccacgctggg cgagacctgg gaagaggccg tgggcgaaaa actcgatcac caggtgctga
     2101 tggataaggt tgtgcgttac acggctgcgg tgccttcccg ggtggtttat ctgacggcgg
     2161 gcattgactc gcagcgaaac cgttttgaga tgtatgtctg gggatgggct ccgggagagg
     2221 aagcctttct ggtggataaa atcatcatta tggggcgtcc cgatgaggaa gagacgctgt
     2281 tacgtgtgga tgtggcgatc aacaaaaaat accgccatgc agacggaacc gaaatgacca
     2341 tttcccgtgt ctgctgggac accgggggga tcgatggcga aattgtctat cagaggtcaa
     2401 aaaaacacgg tgttttccgg gtgctgccgg taaaaggtgc atctgtttat ggcaagccgg
     2461 tgatcaccat gccaaaaacc cgcaattagc ggggcgtgta tctgtgcgaa gtggggacgg
     2521 acaccgcaaa agaaattctc tatgcccgta tgaaagccga tcccacgcct gcggatgaag
     2581 ccacgtcgta tgccatccgt tttcctgatg atccggagat tttttcgcag acagaggcgc
     2641 agcaactggt ggcggaagag cttgtggaga agtgggaaaa aggaaagatg cgtctgctgt
     2701 gggataacaa aaagcggcgt aacgaagcgc tggactgcct ggtgtatgcc tacgcggcat
     2761 tacgtgtgtc cgtgcaacgc tggcaacttg atctggctgt actggcaaaa tcccgggaag
     2821 aagagacgac ccggccaacc cttaaagaac tggcagcgaa gctgtccgga ggagtgaatg
     2881 gttacagtcg ctgaactgca ggcgctgcgt caggcgcgcc ttgatttatt aaccggtaaa
     2941 cgggtggtgt ctgtccagaa agatggtcgc agaattgaat atacggcggc ctctctggat
     3001 gagcttaacc gggcgatcaa tgatgctgag ttggtactgg ggacaacccg ccgtcgccgt
     3061 cgtccgctgg gagtgaggtt atgaaacgaa agcctgtcct gattgatgtg aacggcgttc
     3121 cgcttcggga gagcctcagc tacaacgggg gcggtgcagg atttggcggg caaatggcag
     3181 agtggttgcc accctcgcag agtgccgatg cggccctgct gcccgcgttg cgtctgggga
     3241 atgcccgtgc agatgatctg gtgcgcaata acggaatagc ggccaatgcg gtggccctgc
     3301 ataaggatca cattgtcggg catatgtttc tgattagcta ccgtccgaac tggcgctggc
     3361 tggggatgcg ggagaccgcg gcaaaaagtt ttgtcgatga ggtggaggcg gcctggtcag
     3421 aatacgcaga agggatgttt ggtgagatcg acgtggaagg gaaacgcacg tttacggaat
     3481 ttatccgtga aggtgtgggc gttcatgcgt ttaacggcga aatctttgtg cagccggtct
     3541 gggatacgga gagcacgcaa ctgtttcgta cgcgttttaa agccgtgagt ccgaaacggg
     3601 tggacacgcc aggacacggt atcgggaacc gttttctgcg ggccggggtg gaggttgatc
     3661 gatatggccg tgccgttgcg taccatatct gtgaggatga ttttcctcgc tccgggagtg
     3721 gacgatggga acggatcccg cgtgaacttc ccaccgggcg tccggccatg ctgcatattt
     3781 tcgagccggt ggaggacggg cagacccgtg gggccaacca gttttacagc gtcatggaac
     3841 ggctgaagat gctcgattcc ctgcaggcaa cacagcttca gtcggccatt gttaaagcca
     3901 tgtatgcagc gacgattgaa agtgaacttg ataccgaaaa ggcctttgaa tatatcgccg
     3961 gtgcgccgca ggggcagaag gataatccgc ttattaatat tctggataag ttctccacct
     4021 ggtatgacac gaatagcgtg acgctgggcg gtgtcaaaat tccgcacctt ttccctggtg
     4081 atgatctgaa acttcagacc gcgcaggatt cagacaatgg attttcggcg cttgaacagg
     4141 cgctgctgcg gtatatcgct gccggtcttg gcgtttccta cgaacagttg tcccgtgatt
     4201 actcgaaggt cagttactca agtgcccgcg cctccgccaa tgagtcgtgg cgctatttta
     4261 tggggcggcg aaaatttatt gcggcccggc tggccacgca gatgttttcc tgctggctgg
     4321 aagaggcact tcttcggggg attattcgtc cgccacgggc acgttttgat ttttatcagg
     4381 cgcgttcagc ctggtcacgg gctgagtgga ttggtgccgg aagaatggcc attgacgggc
     4441 tcaaggaggt tcaggaatcg gtgatgcgta ttgattccgg actgagcacg tatgagaaag
     4501 agctggcgct gatgggcgag gattatcagg acattttccg ccagcaggtc agggaatctg
     4561 cagagcggga aaaagccgga ctctcacgtc cggtgtggat agcgcaggcg tatcagcagc
     4621 agatagcgga gagtcgcagg ccggaagagg agacaacacc acgtgagacg taatctttca
     4681 cacattattg cagcagcatt caatgaaccg ctgcttctgg agcccgccta tgcgcgggtt
     4741 ttcttttgcg cgctcgggcg cgagatgggg gcagcaagtc tttcggtacc acaacagcag
     4801 gtacagcttg atgcacccgg aatgctggct gaaacggacg agtacatggc cggaggtaaa
     4861 cgaccggccc gtgtttaccg ggtggtgaac ggtattgctg tactgccggt cacaggcacg
     4921 ctggtgcacc ggctgggggg tatgcggcca ttttccggaa tgacaggcta tgacggcatt
     4981 gtcgcctgtc ttcagcaggc aatggcggat agccaggtgc ggggtgtact gctggacatt
     5041 gacagtccgg gcgggcaggc cgccggtgcg tttgactgtg ctgacatgat ttaccgcctc
     5101 cgtcagcaga agccggtctg ggcactgtgc aatgacacgg cctgttctgc agccatgctg
     5161 ctggcgtcgg cctgctcccg acggctggtt acccagacat cccgtatcgg ctccattggc
     5221 gtgatgatga gccatgtcag ctatgccggt catctggcgc aggccggtgt ggatatcacg
     5281 ctgatttact caggggcgca caaggtggat ggcaatcagt ttgaagcgtt gccggaagag
     5341 gttcgccagg acatgcagca gcggattgat gcggcgcgcc ggatgtttgc cgaaaaagtg
     5401 gcgatgtata ccggtctgtc tgttgatgca gtcacgggaa cagaggccgc cgtttttgaa
     5461 ggtcagtccg gcattgaggc cgggctggcg gatgaattaa tcaatgcgtc ggatgccatc
     5521 agcgtgatgg ctgcggcgct gaacacacat gatacaggag gcactatgcc gcaattaact
     5581 gcaacggaag ctgccgcgca ggagaaccag cgagtgatgg ggatcctggc gtgtcaggaa
     5641 gcgaaaggac gtgaacacct tgccacgatg ctggcaggac aacagggcat gagcgttgaa
     5701 caggcccggg cgattctgac cgcggcagca ccacagcagc cggtggcatc cacgcagagt
     5761 gaagccgatc gcattatggc gtgtgaagaa gctaaaggtc gtgaacaact ggcggcaacg
     5821 ctggcggcga tgccggatat gacggtggaa aaagcccgcc cgatcctggc ggcttcaccg
     5881 caggcggatg ccggaccctc actccgtgat cagattatgg ctctggatga ggcaaaaggg
     5941 gctgaggcgc aggctgaaaa actggcggcg tttcccggaa tgacggtgga ggctgcccgc
     6001 gacattctgt cctcatcgcc ggataaagca gaaccggttt ctgcatccac aaccgccatg
     6061 tttgaacgca tcatggcgaa ccattcaccg gcagccgtgc agggtggcgt gtcacagacg
     6121 tcggcagacg gtgatgcgga cgtgaaaatg ctcatggcca tgccatgaag tcagtgctga
     6181 ccatcaatat gaggttttaa caaaatggtg acgaaaacca tcactgaaca acgtgcggaa
     6241 gtacgtattt ttgccggtaa tgatccggct cataccgcca caggcagcag cgggatttct
     6301 caggcaacac cggctctgac gcccctgatg ctggatgaag ccagcgggaa actggtggtc
     6361 tgggatggac agaaagccgg tagtgcggct ggcatactgg tactgccgct tgaaggcaca
     6421 gagacggtgc tgacctatta caagtcgggg acctttgcga cggaggcaat ccgctggcct
     6481 gacagtgtgg atgaacacaa aaaggcaaat gcctttgccg gtacagccct gagtcacgcg
     6541 gctctgccgt aacacgttat caggccacca tggtggcctg actgatttct gaatgaaagg
     6601 aactgattta tgggattgtt tacgacccgc cagttactcg gttataccga acaaaaagtg
     6661 aaattccgtg cgctgtttct ggagctgttt ttccgccgta cggtgaattt ccacaccgaa
     6721 gaggtgatgc tggacaaaat taccggaaaa acgccggtgg cagcctatgt ctccccggtt
     6781 gttgaaggaa aagtgcttcg ccatcgcggt ggtgaaaccc gcgtgttacg tccgggctac
     6841 gtcaagccca aacacgaatt taattaccag caggcggttg agcgccttcc tggtgaagat
     6901 ccggctcagc tgaatgaccc ggcctaccgt cgtctgcgta tcattaccga taacctcaaa
     6961 caggaagagc acgccattgt ccaggtggaa gaaatgcagg cggtgaatgc cgtgctgtat
     7021 ggcaaataca ccatggaagg ggatcagttt gatactgtcg aggtggattt cgggcgctct
     7081 gaaggaaata acattgagca ggctgacggt aaaaaatggt ctgagcagga ccgtgatacg
     7141 tttgatccga cgcatgatat tgacctctac tgcgatcagg ccagcggcct tgtgaatatc
     7201 gccattatgg acggtacggt ctggcgtctg ctgaatggct ttaagctgtt ccgcgaaaaa
     7261 ctggataccc gtcgcggctc aaattcacaa ctcgaaacgg cagtgaaaga cctgggggcg
     7321 gtggtgtcct tcaaggggta ttacggcgat ctggccattg tggtggcgaa aacgtcttat
     7381 gtggcagagg acggtaccga aaaacgttat ctgccggagg gcacactggt cctggggaat
     7441 acggcagcag agggcattcg ttgctatggt gccattcagg atgcgcaggc gttgtccgaa
     7501 ggtgtggtgg cctcttcccg ttatccgaaa cactggctga ctgtgggcga tccggcccgt
     7561 gaattcacca tgacgcagtc cgctccgctg atggtgctgc cggatccgga tgagtttgtg
     7621 gtggtacagg tgaaataatc cgtgagcggg ggcgaaatgc ccccgtgtct tttttcacag
     7681 gaggctgaga tggcaacaaa agaagaaaat ctgaatcgtc ttcgtcaact ggctggcctg
     7741 ctggggcgcg aggcggatat gtcggggagt gctgcggata ttgctcaacg tgtgtctgag
     7801 tgggaagagg agcttgctgt ttccccggag ggcattatgc actctgatga gagcggggct
     7861 gatcaaaatc acacagacga tggtgagcag ttgaacaaca cggatgctcc ggatgatgtt
     7921 aaagccgtcc gggtacggaa gtgcctgcaa gtaatggggt attgcccgga gacaggtcgt
     7981 cccgttgagc tggcgttacg gggtatgcgt gttctggtgc catcatcact ggcaacggcc
     8041 atgatacagc acggaacggc tgaatatgcg tgattttcag aatgcctttg atgctgccct
     8101 cgccggggtg gacagtacga tcgttgaagt gatggggatc cgtgcgcagt tcacctctgg
     8161 agcacaacgt ggcggcgaag ttcagggggt ttttgacgat ccggagtcgc tgggttttgc
     8221 cggtggcggg gtccgtattg aaggaagcag cccgtcatta tttgtgcgga cggatacggt
     8281 gcgtgccgtg cggcgtggtg acacgctgac cattaacggc gagatgttct gggtggatcg
     8341 tgtttctccg gatgacgggg gaagctgtta tctctggctc aaccgtgggc aaccacccgc
     8401 tgttaaccgg cgacgataaa cgcagggtga aattatggcg ataaaagggc ttgatcaggc
     8461 gattgacaat ctgagccggg ttcgtaaaaa cgccattccg gcggcttcag caatggccat
     8521 taaccgcgtg gccacaacgg cgattaatca gtcttcatca caggttgccc gggagacaaa
     8581 ggttcgccgg aaactggtta aggaacggtc cagactgaaa cgggccacgg tcagaaatcc
     8641 gaatgccaga attatcgtta accgcggtga tctccctgtg attaagctgg ggatcaggat
     8701 gctggggcgt cgcccgaaca gcatacttaa agccggtcag catcggtatc agcgggcatt
     8761 tattcagcga ttaaaaaatg gtcgctggca tgtcatgcag cgtgtggccg ggaaaaaccg
     8821 ttaccccatt gatgtggtga aaatcccgat ggcggcccca ctgaaacagg catttgatga
     8881 gaatgttgac cgtatccggc gtgaacgcct gcctaaagaa ctggcatacg cgctgaaaca
     8941 acaactgagg attgcgataa aacgatgaaa cacactgaca ttcgtgccgc agtgctggat
     9001 gcactcgagc agcatgaaca cggggcgacg ctgtttgatg gtcgccccgt tgtttttgac
     9061 gaagaggatt ttcctgcgat cgcggtttat ctgacggatg cagagtatac cggtgaagag
     9121 ctggatgcag atacctggcg ggccacactg catattgagg tgtttttacc ggcacaggta
     9181 cctgattcgg agctcgatca gtggatggaa agccggattt atccggcgat gactgcgatc
     9241 cctgcactgg caggactgat taccacgatg gttacgcagg gctatgagta tcgtcgtgat
     9301 gacgatatgg cgttatggag ctctgcggat ctgacttatt ccattacata cgagatgtga
     9361 ggacgatatg gcaacaccaa atcccctgga gccggtaaaa ggtgccggta ccactctgtg
     9421 ggtttacaac ggcaagggtg atgcttatgc aaacccgttg tcagacgatg actggcagcg
     9481 actggctaag gtgaaggatc tgacgccggg cgagatgacg gcagaaccct acgatgataa
     9541 ctacctggat gatgaagacg cggactggac cgcgaccggg caggggcaga agtctgcagg
     9601 agataccagt tttacgctgg cctggaaacc gggagaagat ggtcagaaag ggcttatagg
     9661 ctggtttgaa agcggggatg tgcgggccta taaaatccgt tttccgaacg gcacggtgga
     9721 tgtgtttcgt ggctgggtca gcagtatcgg taaggccgtg acggcgaaag aagtgatcac
     9781 ccgcacggtg aaagtgacca acgtgggcaa accttccgtg gcggaagaac gcagcaaaat
     9841 tacgccggtc actgcaatta aggtgacaca gacatccggt accgtggaaa aaggaaaaac
     9901 aaccaccctg acggtttctt ttgagccgga aagtgcaacc gacaagacgt tcagagcggt
     9961 ttccgccgat ccgtcaacgg gaagcattgc tgtgaaagat atggtgatca ctgtgacggg
    10021 gattaaggcc ggaaaagtga gtatccccgt gatttccggt aatggtcagt ttgccacggt
    10081 agctgaagtc accgttactg aagcgggcgc tgcagggtaa acggaggtaa tacatgtttc
    10141 tgaaaacaga acaatttgaa tataacggtg tatccgtcat gctttctgag ctgtctgcgc
    10201 tgcagcgtat tgagcatctt gccctcctga aacggcgggc agaagaggct gaagccagcg
    10261 gcaacctgca ggtgagtgtg gaagatcttg tcagaaccgg cgcgtttctg gtggcgatgt
    10321 ccctgtggca taaccatcca cagaaaacgc agtcaccgtc aatgaatgag gccgtgatga
    10381 agatagagca ggaagtgctc accacctggc ctgccgatgc cattgcccgg gcggaagagg
    10441 tggtgttacg tctgtccggg atgagcgggg ctgttcatgc ggatactgac agcaccgaag
    10501 tggcgaaaaa taacgcgctt actgatgatg atttttctgc gggaaagttt tcgacggcga
    10561 gctgaatttt gccctcagac tggcgcgtga gatggggagg cctgactggc gcgccatgct
    10621 tgccgggatg acatccaccg aatatgccga ctggcgacat ttttaccgca cgcattattt
    10681 tcaggatacc caactggata tgcatttttc cgggctgatg tacgccgtac tcagcctgtt
    10741 tttttgcgat ccggatatgc atccctcgga tttcagtctg ctggctcccc ggcgagagga
    10801 agcgcagacg gagatgccgg atgaggaaga catgctgatg cagaaagcgg caggacttgc
    10861 cggaggcgtc cggttcggtg gggagggagg gggcgatatt tcaccttctg cagatgtggt
    10921 ggatgtcagc gaggatgatg ttgcattaat gatggcttca gcggggattt ccggaggtgt
    10981 gagatatgtc ccagccggtt ggtgatcttg ttattgacct gagtctggat gctgtccgtt
    11041 tcgatgagca gatgagccgg gtaaggcgtc atttttcagg actggatacc gacgccagaa
    11101 aaaccgccgg tgccgttgag cagagtctga accgtcaggc gctggccgca caaaaagccg
    11161 ggatttccgt cgggcagtat aaagcagcca tgcgtaccct gcccgcacag tttacggata
    11221 tcgccacgca gcttgccggt ggtcagaatc cctggctcat cctgctgcaa cagggcggtc
    11281 aggtgaagga ctccttcggc gggatgatcc ccatgttcag ggggcttgcc ggtgcgatca
    11341 ccctgccgat ggtcggggtc acctcgctgg cggtggcgac aggtgcgctg gcgtacgcct
    11401 ggtaccaggg ggattccacg ctttcagaat ttaataaaac gctggtcctt tccggcaatc
    11461 aggccggact gactgccgat cgtatgctga cgctctcaag agccgggcag gcagcagggc
    11521 tgacgtttaa ccaggcgaga gagtcactgg cagccctggt gaatgccggt gtgcgtggtg
    11581 gtgaacagtt tgatgccatt aaccagagtg tcgcgcgttt tgcgtctgca tccggtgtgg
    11641 aggtggataa agtcgctgaa gccttcggga agctgaccac tgacccgacg tcgggactga
    11701 tggcgatggc gcgccagttc cgtaacgtga cggcagagca gattgcgtat gttgcacaac
    11761 tgcagcgttc cggagacgag gccggggcct tacaggcggc gaacgatatc gccacgaaag
    11821 gctttgatga gcagacccgt cgcctgaaag aaaacatggg aacactggag acctgggcgg
    11881 ataaaacagg gaaggcattc aaatcgatgt gggatgccat tctggatatc ggtcgtccgg
    11941 aatcctcagc ggatatgctc gccagtgcgc agaaggcatt tgatgaggcg gataaaaaat
    12001 ggcagtggta ccagagccgg agccagcgcc gcggtaaaac cgcctcgttc cgggccaacc
    12061 ttcagggtgc atgggatgac cgggaaaatg cccgtctggg gctggcggcg gcaacgctgc
    12121 agtcggatat ggaaaaagcc ggtgaactgg cggcaaggga cagggctgag cgtgaggcgt
    12181 cacagctgaa gtataccgga gaggcgcaga aagcgtatga acgcctgctg tcgccgctgg
    12241 agaaatatac cgcccgtcag gaagaactga acaaggccct gaaggacggg aaaatcctgc
    12301 aggcggatta caacacgctg atggcggcgg cgaaaaagga ttatgaatcg acgctgaaaa
    12361 agccgaagtc gtcaggtgtt aaagtgtcag ccggtgagcg tcaggaagac cgggcgcatg
    12421 cggcgctgct ggcgcttgaa accgagctca ggacgctgga aaagcatgcc ggtgcgaatg
    12481 aaaaaatcag ccagcagcgg cgggatttat ggaaagcgga aaatcagtat gtggtcctga
    12541 aagaggccgc cacgaaacgg cagttatctg agcaggaaaa atccctgctg gcccatgaga
    12601 aagagacgct ggagtacaaa cgccagctgg ctgagctggg cgacaaggtt gaacaccaga
    12661 agcggctgaa tgagctggca cagcaggcgg cgcggtttga acagcagcaa agcgcgaagc
    12721 aggccgccat cagcgcaaaa gcccgcggcc tcaccgaccg tcaggcgcag cgggagtcgg
    12781 aagagcagcg ccttcgtgac gtgtacggtg ataatccggc tgcgctggcg aaggccacat
    12841 cggcactgaa gaacacctgg tctgcggagg agctgcttcg tggaagctgg atggccggga
    12901 tgaagtccgg ctggggcgag tgggcggaaa gtgcgacgga cagtttttcg caggttaaaa
    12961 gcgcggccac gcagaccttt gacggtattg cacagaatat ggcggcgatg ctgaccggtg
    13021 cagaggcaga ctggcgggga ttcacccgtt cggtgctgtc catgatgaca gaaatcctgc
    13081 ttaaacaggc catggtgggc attgtcgggc gtatcggcag cgccattggt ggtgctttcg
    13141 gtggtggggc gtctgcctcc acggggacgg ccattcaggc tgcggcggcg aactttcatt
    13201 ttgcgaccgg cggatttacg ggcacgggcg gcaaatatga gcctgcgggg atagttcacc
    13261 gcggggagtt tgtcttcacg aaggaggcaa ccagccggat aggcgtgggg aatctttacc
    13321 gtctgatgcg cggctatgcg gaagggggtt atgtgggtgg tgccggaagt ccggcgcaga
    13381 tgcggcgggc ggaaggtatt aattttaatc agaacaatca cgtggtgatt cagaacgacg
    13441 gtatcaacgg acaggcgggg ccgcagctga tgaaggcggt gtatgacatg gcccgcaagg
    13501 gggcgcagga tgagctccgg ctgcagttgc gtgatggcgg tatgttatca gggagcgggc
    13561 gatgaaaacc tttcgctgga aagtgaagcc ggatatggag gtgaactcgc agccatcggt
    13621 gcgtgaagtg cgttttggtg acgggtactc acagcgtatg gcggcagggc tgaatgctga
    13681 cctgaaaaca taccgggtga tgctttccgt gagccgggag gaggcccggc atctggaagc
    13741 gttcctggca gagcacgggg gctggaaggc atttttgtgg aagccaccct atgcataccg
    13801 gcagataaag gtgacctgtg ccgggtggtc tgcgcgggtc gggatgttgc gcgttgagtt
    13861 cagcgcggag tttaagcagg tggtgaactg atgcaggata ttcacgaagc aagcctgaac
    13921 gagtcggtta agtcagagca gtcaccgcgg gtggtgctct gggaaatcga cctgacggtg
    13981 cagggcggtg agcggtattt tttctgcaat gagctgaatg aaaaagggga gccggtgacc
    14041 tggcaggggc ggaagtatga ggcgtacccg attgagggca gcggctttga gatgaacgga
    14101 aagggcagca gtgcccgccc gtcgctgacg gtgtccaatc tgtttggcct tgtcaccggg
    14161 atggcggagg atttgcagag cctggtgggt gccacggtgg tccgccgccg ggtgtatgcg
    14221 cgttttctgg atgcggtgaa ctttgtggca ggcaatcctg aggcagaccc ggagcaggag
    14281 ctgacggacc ggtgggtggt ggagcagatg tcagcgctga cggccatgac ggcctcgttt
    14341 gtgctggcca caccgaccga gacggacggt gctttgtttc ccggccgcat catgctggcg
    14401 aacacctgta tgtggaccta ccgctctgat gagtgtggtt acacgggcgg ggcggtggcg
    14461 gatgagttcg ataaacccac caccgatatc cgtaaggaca gatgcagcaa gtgcatgcgc
    14521 gggtgtgaac tgcgcaggaa tgtcggcaat tttggcggtt tcctttccat taataaactt
    14581 tcgcagtaaa tcccggttta tgacacagac tgaatcagcg attctggcac atgcccggcg
    14641 gtgtgcgcct gcggagtcgt gcggcttcgt gataagcacg ccggaggggg agcggtatat
    14701 cccttgtgtg aatatttccg cagagccgga ggcgtatttt cgtatcgcac cggaagactg
    14761 gctgcgggca gagatgcagg gggagattgt ggcactggtc cacagtcatc ccggtgggct
    14821 gccctggctg agcgaggctg accggcggct gcagataaaa agtgccctgc cctggtggct
    14881 ggtctgccgg ggtgacattc acaaattccg ctgcgttccg cacctgaccg gacggcgctt
    14941 tgcgcacggg gtgacggact gttacacgct gttccgggat gcataccatc tggcggggac
    15001 tgaaatgccg gattttcacc gcgaggatga gtggtggcgc aacggccaga acctgtacct
    15061 ggacaatatg gaggccacgg gcttttaccg ggtgcccctg tcctctgcac aggcgggcga
    15121 tattctgctg tgctgctttg gcgcatcggt ggccaaccat gccgccattt actgcggcaa
    15181 cggtgaactg cttcaccatc tgcctgaaca actgagtaaa cgggagaggt attccgaaaa
    15241 atggcaacga cgaacgcatt cagtctggcg tcaccgccac tggcacgcat ctgccttcac
    15301 ggggatttac aacgatttgg ccgccgcctc agcctgtatg tgaacacggc agcggaagcc
    15361 atccgcgcac tgtcgatgca ggtgccgggc tttcgccgtc agatgaacga aggctggtac
    15421 cagatacgta ttgccggtga ggacacggca ccggaggcgg tgtatgcccg ccttcacgaa
    15481 cagctgggtg agggaacggt catccacatt gtgccgcgac tggccggtgc cggaaagggc
    15541 ggactgcaga ttgtgctggg ggcggcggcc atcgtggggt cgttcttcac ggccggagcc
    15601 acgatggcgt tatggggttc agccctggca gccggtggtt tttctgccac cacgatgctg
    15661 ttttcactgg gtgccagcat gattctgggc ggtgtggccc agatgctggc cccgaaggca
    15721 aaaacaccgg attaccgcgc aacggataac ggcagacaga acacgtactt ttcctcgctg
    15781 gataacatga ttgcccaggg taacccgatg ccggtgcctt acggtgaaat gctggttggt
    15841 tcacgacgga tatcccagga catcagcacc cgtgatgaag gcggtgacgg gaaagtggtg
    15901 gttatcggac ggcaggggta aaaagaataa aaaaatcccg cagtgatcgc ggagctgcgg
    15961 gagattcaga caaatgaaga ttagtgttaa ggagttgttt tttatgtcac ggcaaaaaaa
    16021 cactaacgca gcgaaattat cagcgccaca gtcagtttgt gaaaatgtga agatattcag
    16081 aatttttatt ccgtcatgat gcaggcactc tccggggtgc ctgttgtttt tatacatcaa
    16141 cagtgtccgg cagcggacga tgaaggggga ttccgtgggc aaaggcggcg gcaaggcgca
    16201 cacaccgcgt gaggcgaagg ataatctcaa atccacgcag atgatgagcg tgattgatgc
    16261 catcggtgag ggaccggtgg aaggcccggt gaagggactg cagagtattc tggtgaacaa
    16321 aaccccgctg acggacacgg acggtaatcc cgtgatacac ggtgtgaccg ccgtctggcg
    16381 tgccggggag caggagcaga caccgccgga aggctttgag tcctccgggg cggaaaccgc
    16441 actgggcgtg gaggtgacga aggcaaagcc ggtgacgcgc accatcacgt cagcgaacat
    16501 tgaccgtctg cgggtcacct tcggggtgca gtcactggtg gagaccacct caaagggtga
    16561 ccgtaacccc tcttctgtcc gcctgctgat tcagcttgag cgtaacggta actgggtgac
    16621 ggagaaggat atcaccatta acggcaagac tacctcgcag tacctgacgt cggtgattct
    16681 ggataatctg ccgccccggc cctttaacat ccggatggtc agggagacgg cggacagcac
    16741 cacggaccag ctgcagaata agacgctctg gtcgtcatac accgaaatca tcgatgt
//
LOCUS       DAZFQD010000115        16608 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.115, whole genome shotgun sequence.
ACCESSION   DAZFQD010000115 DAZFQD010000000
VERSION     DAZFQD010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16608
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.115"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..100)
                     /locus_tag="ACHKTW_003790"
     CDS             complement(<1..100)
                     /locus_tag="ACHKTW_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6464728.1"
                     /translation="MKKVLLQNHPGSEKYSFNGWEIFNSNFERMIKE"
     gene            complement(228..2189)
                     /gene="topB"
                     /locus_tag="ACHKTW_003791"
     CDS             complement(228..2189)
                     /gene="topB"
                     /locus_tag="ACHKTW_003791"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFO6464729.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQSVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRALL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKILRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEAGSGAIA"
     gene            complement(2194..3237)
                     /gene="selD"
                     /locus_tag="ACHKTW_003792"
     CDS             complement(2194..3237)
                     /gene="selD"
                     /locus_tag="ACHKTW_003792"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HFO6464730.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYDAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKATAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            complement(3354..3905)
                     /gene="ydjA"
                     /locus_tag="ACHKTW_003793"
     CDS             complement(3354..3905)
                     /gene="ydjA"
                     /locus_tag="ACHKTW_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HFO6464731.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGRERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCEENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            4066..5922
                     /gene="sppA"
                     /locus_tag="ACHKTW_003794"
     CDS             4066..5922
                     /gene="sppA"
                     /locus_tag="ACHKTW_003794"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFO6464732.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKPVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            6089..7105
                     /gene="ansA"
                     /locus_tag="ACHKTW_003795"
     CDS             6089..7105
                     /gene="ansA"
                     /locus_tag="ACHKTW_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFO6464733.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMGGYATGNALAHAGVIGGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            7116..7757
                     /gene="pncA"
                     /locus_tag="ACHKTW_003796"
     CDS             7116..7757
                     /gene="pncA"
                     /locus_tag="ACHKTW_003796"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HFO6464734.1"
                     /translation="MPPRALLLVDLQNDFCAGGALAVPEGDSTVDVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHCVEPYMPGQLDGLPQTFWPDHCVQNSEGAQLHPLLNQ
                     KAIAAVFHKGENPLVDSYSAFFDNGRRQKTSLDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(7918..8190)
                     /gene="yeaC"
                     /locus_tag="ACHKTW_003797"
     CDS             complement(7918..8190)
                     /gene="yeaC"
                     /locus_tag="ACHKTW_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HFO6464735.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKENCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            complement(8232..8645)
                     /gene="msrB"
                     /locus_tag="ACHKTW_003798"
     CDS             complement(8232..8645)
                     /gene="msrB"
                     /locus_tag="ACHKTW_003798"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFO6464736.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSETKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            8987..9982
                     /gene="gapA"
                     /locus_tag="ACHKTW_003799"
     CDS             8987..9982
                     /gene="gapA"
                     /locus_tag="ACHKTW_003799"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFO6464737.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            10066..10950
                     /gene="yeaD"
                     /locus_tag="ACHKTW_003800"
     CDS             10066..10950
                     /gene="yeaD"
                     /locus_tag="ACHKTW_003800"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HFO6464738.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHREDADGVTLTFELTQSEETKKFWPHDFTLLAHFRVGKTCEIDLESHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSVSMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(11001..11855)
                     /gene="yeaE"
                     /locus_tag="ACHKTW_003801"
     CDS             complement(11001..11855)
                     /gene="yeaE"
                     /locus_tag="ACHKTW_003801"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal reductase YeaE"
                     /protein_id="HFO6464739.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTDLRDNVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFAFEETVAAMEKLIAQGKIRRWGVSNLDYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHA
                     HNISAAQVLLAWVISHQGVMAIPKAATIAHVQQNAAVLEVELSSAELIMLDKAYPAPK
                     GKTALDMV"
     gene            complement(11945..12691)
                     /gene="mipA"
                     /locus_tag="ACHKTW_003802"
     CDS             complement(11945..12691)
                     /gene="mipA"
                     /locus_tag="ACHKTW_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HFO6464740.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            13127..15061
                     /gene="yeaG"
                     /locus_tag="ACHKTW_003803"
     CDS             13127..15061
                     /gene="yeaG"
                     /locus_tag="ACHKTW_003803"
                     /EC_number="2.7.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011411173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HFO6464741.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQEPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNESEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMNGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQQIEREQFPQEQAERYLEFLKGYLIPKYAEFIGKEIQTAYLESYSEYGQNIFDRYVT
                     YADFWIQDQEYRDPDTGQLFDRESLNAELEKIEKPAGISNPKDFRNEIVNFVLRARAN
                     NSGRNPNWTSYEKLRTVIEKKMFSNTEELLPVISFNAKTSTDEQKKHDDFVDRMMEKG
                     YTRKQVRLLCEWYLRVRKSS"
     gene            15174..16457
                     /gene="yeaH"
                     /locus_tag="ACHKTW_003804"
     CDS             15174..16457
                     /gene="yeaH"
                     /locus_tag="ACHKTW_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HFO6464742.1"
                     /translation="MTWFIDRRLNGKNKSMVNRQRFLRRYKAQIKQSISEAINKRSVT
                     DVDSGESVSIPTEDISEPMFHQGRGGLRHRVHPGNDHFVQNDRIERPQGGGGGSGSGQ
                     GQASQDGEGQDEFVFQISKDEYLDLLFEDLALPNLKQNQQRQLTEYKTHRAGYTANGV
                     PANISVVRSLQNSLARRTAMTAGKRRELHALEENLAIISNSEPAQLLEEERLRKEIAE
                     LRAKIERVPFIDTFDLRYKNYEKRPDPSSQAVMFCLMDVSGSMDQSTKDMAKRFYILL
                     YLFLSRTYKNVEVVYIRHHTQAKEVDEHEFFYSQETGGTIVSSALKLMDEVVKERYNP
                     AQWNIYAAQASDGDNWADDSPLCHEILAKKLLPVVRYYSYIEITRRAHQTLWREYEHL
                     QSTFDNFAMQHIRDQDDIYPVFRELFHKQNATAKD"
BASE COUNT         4103 a         4289 c         4073 g         4143 t
ORIGIN      
        1 tttctttaat catccgttca aaattactat taaatatttc ccagccatta aaagaatact
       61 tctcgctccc aggatggttt tgtaataaaa cttttttcat cacattccct gttattacat
      121 actttagagg cgataatttt atccatgcaa aaaaaatatc caacgaattt tcttgatccg
      181 ctggatattt cagaatatga ctcgaatagc acgaaagatt cgctcgctta cgctatcgcc
      241 ccgcttccgg cttcatctgc tggcggactt tttttcgcac tacgtttacg cggtgcagcc
      301 tttttcttat cagcactgcc accactgccc ggagccacaa tgccgcgaaa ctgccgcacc
      361 ggagtacgtt tggcttgatc aataagctga tatagcgtcc ccaccagcgg ctgcataaag
      421 tcctgatagc gacactgctt ttcgctgatt tgcgtcagca ccgattccca gtgcgcggtc
      481 atgtccggtc gcgtagccat ttctggcagc gaatggaata acgcttttcc ggcgtcggtg
      541 gagtggatat agcgcccttt tttggtgagg aaaccacgct tgaacaacag ttcaataatc
      601 ccggcacgcg ttgcttctgt ccccagacca tcggtcgcac gaaggatctt tttcagatct
      661 ttgtcctgca caaagcgcgc aatcccggtc atcgccgaaa gcagtgttgc atcggtaaaa
      721 tggcgcggcg gctgggtttg ccgctctacc acttcacctt tttcacacag caactcatcg
      781 cctttcgcca ccacaggcaa tggcgtgccg tcgttttctt catcgcgctc tttgctgcct
      841 aacagcgcgc gccaacctgc ttcagcaaga aaacgcgctt tagcgacaaa tttgcctttg
      901 gcaatgtcca gttcgataac acacttgcgg aacaccgcat ccgggcagaa ctgcatcaga
      961 tactgacggg caatcaggtt atagaccttc gcttcgttct ccgtcaggtt gatcgcagaa
     1021 ctccttgcgg tcggaatgat ggcgtggtgc gcatcgacct ttttgtcatc ccaacagcgg
     1081 ttgcgtatat ctggatctac cactgactgc ggcaacagat ccggtgcatg aacgctgatg
     1141 gcattcatca ccgcgtggcg tccggcaaaa tgttcttctg gcaaatagcg acaatcggaa
     1201 cgcggataag tgattagctt gtgcgtttcg tacagcttct ggcagatatc aagcacgttc
     1261 tgcgcgctca gaccgaagcg ttttgccgct tcaatctgca acgccgaaag cgaaaacggc
     1321 agcggcgcgg attctgattc ccgtttatcg ttatagctgg tgacaatagc cggttgacca
     1381 ctaatgcggt taaccacatg ctccgccagt ggacgatgta acaagcgccc ttcttcatcc
     1441 tggtacggtt cacacgcttc gctcggttgc cagatagcgg taaaccgctc atcggcaggt
     1501 gtcacgatat gcgctttgac ttcaaagaag tctttcgcca cgaagttttc aatctcttca
     1561 tcgcggcgca ccaccagccc gagcacgggt gtctgcacgc gtcccacgga aagtacgccc
     1621 tgataaccgg cattgcgacc gagaatggta tacgcacggg tcatattgat gccgtacagc
     1681 cagtcggcac gcgctcgcgc cagcgcagaa acgcacagcg gtacaaactc actgttggaa
     1741 cgaagacggt cgatcgcccg ctcaaccgcc tgcgggttca ggtcgtttat caagcaacgc
     1801 tgcacctgct ggcgcttttc cggtgccagt tgtagatagt ccagcacttc atccaccagc
     1861 agttgccctt cgcgatccgg gtccccggcg tgaacgattt cgctggcttc atgcaggaac
     1921 cgtttgatga cgttaagttg tttggtcacg gagggtcggg gctgtaattg ccacttttcc
     1981 gggacaatcg gcaaatccgc aagattccag cgcgcatagc ggctgtcgta ggcgtctggc
     2041 tgcgcctgct caagcaggtg accgatacac caggtcacca cctgaccatt accgcactcg
     2101 ataaagccat cgcctttacg gtgcggtttg ggcaggacat cagcaatggc gcgcgccaga
     2161 ctcggttttt cggcaataaa caaccgcatt gaattaacgg atctcaacca tggcacgacc
     2221 gccgcgcgct ggcaccagtt cgccaatcgc cgtcagttca atgccaaact cggcggctgt
     2281 agctttaacc tcattttctg cttccggcat gaccgccagc agcaaaccgc cagaagtttg
     2341 cggatcgcac agcagatcgc gcacttcacg cggcatttca cccatcagat gaccgtagct
     2401 ggcaaagtta cgttcggtgc cgcctggcac tgcgcccaac ttaatgtact cttcaacccc
     2461 cggcaatttc gggatcgcgt catagtcaac gcgcgcctgc acacctgcgc cctgacacat
     2521 ttcgctcaaa tggcccagca gaccaaagcc cgtaacgtcg gtcatcgctt ttacgccttc
     2581 gatgttggca aaggacgcgc ctgcgatatt catccggcac atcacttctg tcgccagtcc
     2641 ctgatgttct ggtttcagca gtgatttttt ctcagccgtg gtaagaacgc cgatccccag
     2701 cggtttcgtc aggaacagtt tgcatccggc ttgtgcggta ctgtttttct tcacccgctc
     2761 ggtcggtacg atccccgtta ccgccagacc aaaaatcggc tccggcgcat cgatggagtg
     2821 accgcccgcc agcgcaatac ccgcctgacg gcatgcatag cgtccacctt cggttacttc
     2881 gcgggcaatt tccggggaaa gtttgttaat cggccagccg aggatcgcaa tcgccataat
     2941 cggtttgccc cccatcgcga agatatcgct gatggcgtta gtcgccgcaa tgcggccaaa
     3001 atcgaaagga ttatcaacga tcggcataaa gaagtcggtg gtactgataa cgctggtgcc
     3061 attgcccaga tcgtacaccg ccgcatcgtc gcgggtctca ttacccacaa gcaaattcgg
     3121 atcaacaaac ttcgcctgct cactgtgcag gatggtttcc aacacttttg gggaaatttt
     3181 acagccgcaa ccagctccgt ggctgtattg ggtcaaacga atcgagttct cgctcatgga
     3241 catctcctgt caatgcaatc cgggtatggt aaccctcatt ccgtgaagtg ataagtgaga
     3301 gtgtctgaat tcctgcgcct ttgctcacaa tccagacagt ttcgcgacaa ttatcagaaa
     3361 taagttacaa acggcgtcgg gtccgggacg ttaatcgacg tagatgcttt caactgcggc
     3421 gtgccgaggt agagaaaacc gacaatttta tcctgctcac ggcaaccgaa tgcttcacgc
     3481 actaccggac tttcagttaa tgcgccactg cgccagatgc cgccaaatcc ttgggcaacg
     3541 gctgccattt gcatcgccat gaccgcgcat ccggcagaca tttcctgttc ccatcgcggg
     3601 actttatgat tttcttcgca tttcgccacc accgtgatga tgagcggtgc gcggaacggc
     3661 gcattacggg ctttgtcgat agctttgtca tcactaccgg cagcaatcgc cccctgttcc
     3721 agtacggcgc tgaaacgctc gcgcccttcc ccttcaatca caaaaaaatg ccacggttgc
     3781 atggacttat ggtccggcgc acgcataccc gcacgcagga tgttttgcag ttgttcaccc
     3841 gttggcgcgg gttcagccag gcgggaggcg ctacggcgat tgatcaatag ttcgagtgca
     3901 tccatttgat taactcctgt cgtgatattt attcacaaaa ttaacacgag agtggatttt
     3961 gttacagcac agtccgcaat tcctgctgac aagtgccggt tgggtcatta cgataaccac
     4021 atctattgcg cctgtgacag gtgtgacctt aagttgggag aatacatgcg aaccctttgg
     4081 cgatttattg ccggattttt taaatggacg tggcgtctgc tgaatttcgt ccgtgaaatg
     4141 gtacttaacc tgttctttat tttcctcgta ctggttggtg tggggatctg gatgcaggtc
     4201 agtggtggtg attcgaaaga aacggccagt cgtggcgcac tgctgctgga catttctggt
     4261 gtgatcgtcg ataaacccga cagttctcag cggtttagta aattaagccg ccagctgctt
     4321 ggtgccagtt ccgatcgtct gcaggaaaac tcactgtttg atatcgtcaa caccattcgc
     4381 caggcgaagg acgaccgcaa tatcaccggt atcgtgatgg atctgaaaaa cttcgcaggc
     4441 ggcgaccagc cgtctatgca gtacatcggc aaagccctga aagagtttcg tgacagcggg
     4501 aaaccggttt atgccgttgg cgagaactac agccaggggc aatattatct cgccagtttc
     4561 gccaataaaa tttggctgtc accgcaaggc gtggttgatc tgcacggttt tgctaccaac
     4621 ggtctgtact acaaatcgtt gctggataag ctgaaagttt ccacccatgt gttccgcgtg
     4681 ggtacgtata aatctgccgt tgaaccgttt attcgtgatg atatgtcacc ggcagcccgc
     4741 gaagctgaca gccgctggat tggtgagctg tggcaaaact atctgaatac tgttgccgct
     4801 aaccggcaga tccctgctca gcaggtattc cctggcgcgc aagggttgct tgagggttta
     4861 accaaaaccg gtggcgatac cgcgaaatat gcactggaaa acaagctggt cgatgcactg
     4921 gcatcgagtg ccgaaatcga aaaagctctg accaaagagt tcggctggag taagactgat
     4981 aaaaattatc gcgccatcag ttattacgat tacgcattga aaacgccggc agataccggt
     5041 gacagcatcg gtgtcgtctt cgctaatggc gcaattatgg atggcgagga aactcagggg
     5101 aatgttggcg gtgataccac tgcggcacaa atccgcgacg ctcgccttga cccgaaagtg
     5161 aaagcgattg tcctgcgtgt taatagccca ggcggcagcg ttaccgcgtc tgaagtgatt
     5221 cgcgctgaac tggcagcagc ccgggcagcg ggtaagcctg tggttgtatc gatgggcggc
     5281 atggcggcat ctggtggtta ctggatttcc acgccagcta attacattgt ggctaacccc
     5341 agcaccctga ccggttctat cggtatcttc ggcgtgatca ccaccgtaga aaatagtctg
     5401 gattcgattg gtgttcatac tgatggtgtc tcaacttcac cgctggcgga tgtttctatc
     5461 accagggcac tgccgccgga agcgcagcag atgatgcaat taagcattga gaatggctat
     5521 aaacgcttta tcacgctggt tgctgatgcg cgtcattcga cgccggagca aattgataaa
     5581 atcgcccagg gccacgtctg gaccggtcag gatgcaaaag ctaacgggct ggtcgatagt
     5641 ctcggggatt tcgatgatgc ggttgccaaa gcagcagagc tggcaaaagt gaaacagtgg
     5701 catctggaat actacgttga tgaaccgacc ttcttcgaca aagtgatgga caacatgtct
     5761 ggttctgtcc gggcaatgtt gccagatgcg ttccaggcca tgttacctgc accgcttgcc
     5821 tcggtagcct ctaccgttaa aagtgaaagc gacaagctgg ccgcgtttaa cgacccacaa
     5881 aaccgttatg cgttttgcct gacctgcgcc aacgtgcgtt aagtcttgta ctgagtggcc
     5941 gacagatcgt cggccacatt attttttacc tcgacgaatc ctcttcccgc tgtctcgacc
     6001 catatttcct tatccgcata gtatcaggtg cgctcctcct gcctcacgta tatacttttg
     6061 ctctttcgat atcattcata tcaatatcat gcaaaagaaa tcaatttacg tcgcctacac
     6121 gggcgggacc atcgggatgc agcgttccga gcagggttat attccggtgt caggtcatct
     6181 acaacgccaa ctggcgctga tgccggaatt ccatcgcccg gagatgccag atttcaccat
     6241 tcatgaatat acgccgttga tggattcttc tgatatgacg ccggaagact ggcagcatat
     6301 tgctgaagat attaaagcgc actatgacga ctatgatggt tttgtcattc tacacggtac
     6361 cgacacgatg gcgtataccg cctctgcgct gtcgttcatg ctcgagaatc tcggtaaacc
     6421 ggtcattgtg acagggtcac aaatcccgct ggctgagtta cgctctgacg gacaaattaa
     6481 tctgctgaat gcgttgtacg ttgcggcgaa ttatccgatc aacgaagtaa cgctcttttt
     6541 caataaccga ttgtatcgcg gcaaccgcac taccaaagcc catgccgatg gttttgatgc
     6601 gtttgcctct ccaaaccttc ctccgttact ggaagctggt atccatattc gtcgtttgaa
     6661 tacgccaccc gccccgcacg gtgaagggga attaatcgtt catccaatca cgccacaacc
     6721 aattggcgta gtgacgattt atccggggat ttctgctgat gtcgtgcgca attttctgcg
     6781 ccaaccggtg aaagcattga ttctgcgctc ctatggcgtg ggtaatgcgc cacaaaacaa
     6841 agccttcctg caggaattac aagaagccag cgatcgcggt attgtggtgg ttaacctgac
     6901 acaatgtatg tccggtaaag tgaacatggg tggttatgcc accggtaacg ccctcgccca
     6961 tgccggcgta attggcggtg cagatatgac tgtagaagct acgctaacca aactgcatta
     7021 cctgctgagc caggaactgg atactgaaac cattcgcaag gccatgagcc aaaacctgcg
     7081 cggcgaactg acgccggatg attaaggaga ctgcaatgcc ccctcgcgcc ctgttactgg
     7141 tcgatttaca aaatgatttc tgtgctggtg gcgcgctcgc cgtgccggaa ggtgacagta
     7201 cggtggatgt cgctaaccgc cttattgact ggtgccagtc gcgcggtgaa gcggttatcg
     7261 ccagtcagga ctggcacccg gcgaatcacg gcagttttgc cagtcagcac tgtgtagagc
     7321 cttatatgcc aggtcaactc gacggtttgc cacaaacctt ctggccagat cactgtgtgc
     7381 agaacagtga aggcgcacaa ttacatccgt tactgaacca aaaagcgatc gcagcggtgt
     7441 tccataaagg cgaaaatcct ttagttgaca gttacagtgc cttttttgat aacggccgtc
     7501 ggcagaaaac ctctctcgat gactggttac gcgatcatga aatcgatgaa ttgatcgtta
     7561 tgggcctggc tactgactat tgcgtgaagt ttaccgtgct ggacgcgtta cagttaggtt
     7621 ataaagtaaa cgtgattacc gatggttgtc gtggcgtgaa tatccagccc caggacagtg
     7681 cgcacgcgtt tatggagatg tcagcagctg gggcaacgct atatacgctg gcagactggg
     7741 aagagacgca ggggtaatat tagttgaatt catctctggc ctacatttcc tcaatgacat
     7801 tgtaggccag ataatacttg tcagtgttgc atttaaccta cttgcagcta aaacacgacg
     7861 tcctaacaag tatatttgcc ctcccttaat aacaggctta aatactagat aactcattca
     7921 cttaaacata gcaataggct ttgctgagat accaaaatct tcttttaact gctgtttgct
     7981 cttcatgacc atctgaccat ttgtgtcaat tgtcatatgc tgtgcttcgg tattatggcg
     8041 ggcttgccac agcatcacca gttgcaggca attctctttt tgttcctcgg tcaacgcaac
     8101 gccatcaggc catttcccca gttcaacggc ggtcgacaaa cgctggtata cctcaggcgt
     8161 catgctgttg ataatgtcat caagattcat aatttttccg ctcctgtgga ataagttgct
     8221 gaatcgtttt ttcaaccgtt gatttcttcg ccgttttcgc catcagtgaa gcgtaaagaa
     8281 gcagagttaa cacaataacg ttcgcctgtt ggttgcggcc cgtcgggaaa gacatgcccc
     8341 agatgggcat cacagttacc gcaacgaatt tctatgcgct gcattccatg tgacaagtct
     8401 ttgatataac gaatggattc ttcactcacc ggttcgtaga aactgggcca gccacagccg
     8461 gaatcatact tggtttcgga atgaaacagc ggggcatcgc agatcaaaca gtgatatacg
     8521 ccgtcacgct tgttatgcag taaacgaccc gtaaatggcg gttctgtccc atgattctgc
     8581 gtcacgtaaa actgcatctc ggacaaattt tttttcagtt cttctgccga aggtttatta
     8641 gccatttgct cacatctcac tttaatcgtg ctcacattac gtgactgatt ctaacaaaac
     8701 attaacacca actggcaaaa ttttgtccta aacttgatct cgacgaaatg gctgcaccta
     8761 aatcgtgatg aaaatcacat ttttatcgta attgcccttt aaaattcggg gcgccgaccc
     8821 catgtggtct caagcccaaa ggaagagtga ggcgagtcag tcgcgtaatg cttaggcaca
     8881 ggattgattt gtcgcaatga ttgacacgat tccgcttgac gctgcgtaag gtttttgtaa
     8941 ttttacaggc aaccttttat tcactaacaa atagctggtg gaatatatga ctatcaaagt
     9001 aggtatcaac ggttttggcc gtatcggtcg cattgttttc cgtgctgctc agaaacgttc
     9061 tgacatcgag atcgttgcaa tcaacgacct gttagacgct gattacatgg catacatgct
     9121 gaaatatgac tccactcacg gccgtttcga cggtaccgtt gaagtgaaag acggtcatct
     9181 gatcgttaac ggtaaaaaaa tccgtgttac cgctgaacgt gatccggcta acctgaaatg
     9241 ggacgaagtt ggtgttgacg ttgtcgctga agcaactggt ctgttcctga ctgacgaaac
     9301 tgctcgtaaa cacatcactg ctggtgcgaa gaaagtggtt atgactggtc cgtctaaaga
     9361 caacactccg atgttcgtta aaggcgctaa cttcgacaaa tatgctggcc aggacatcgt
     9421 ttccaacgct tcctgcacca ccaactgcct ggctccgctg gctaaagtta tcaacgataa
     9481 cttcggcatc atcgaaggtc tgatgaccac cgttcacgct actaccgcta ctcagaaaac
     9541 cgttgatggc ccgtctcaca aagactggcg cggcggccgc ggcgcttccc agaacatcat
     9601 cccgtcctct accggtgctg ctaaagctgt aggtaaagta ctgccagaac tgaatggcaa
     9661 actgactggt atggcgttcc gcgttccgac cccgaacgta tccgtagttg acctgaccgt
     9721 tcgtctggaa aaagctgcaa cttacgagca gatcaaagct gccgttaaag ctgctgctga
     9781 aggcgaaatg aaaggcgttc tgggctacac cgaagatgac gtagtatcta ccgatttcaa
     9841 cggcgaagtt tgcacttccg tgttcgatgc taaagctggt atcgctctga acgacaactt
     9901 cgtgaaactg gtatcttggt acgacaacga aaccggttac tccaacaaag ttctggacct
     9961 gatcgctcac atctccaaat aagttgagat gacactgtga tctaaaaaga gcgacttcgg
    10021 tcgctctttt ttttacctga taaaatgaag ttaaaggact gcgtcatgat taagaaaatt
    10081 tttgcccttc cggtcatcga acaaatctcc cctgtcctct cccgtcgtaa actggatgaa
    10141 ctggacctca ttgtggtcga tcatccccag gtaaaagcct cttttgcatt acagggcgca
    10201 caccttctct cgtggaaacc tgcgggtgaa gaagaagttc tgtggttgag caacaacaca
    10261 ccgttcaaaa atggcgtcgc tattcgcggt ggcgtaccgg tttgctggcc gtggtttggt
    10321 ccggcggcac aacaaggtct gcctgcgcac ggttttgccc gcaacctgcc gtggacgctg
    10381 aaatcacatc gtgaagatgc tgatggcgta acgctgactt ttgaattgac gcaaagcgaa
    10441 gagacgaaaa aattctggcc gcacgacttt acgctgttag cgcatttccg cgtgggtaaa
    10501 acctgtgaaa tcgatcttga atcacatggc gaatttgaaa ccacctctgc cctgcatacc
    10561 tactttaacg tgggtgatat cgctaaggta agcgtcagtg ggctgggcga tcgcttcatt
    10621 gataaagtaa atgacgcgaa agaagatgta ctgaccgacg gtattcagac cttccctgac
    10681 cgtaccgatc gcgtgtatct gaatccgcaa gattgcagcg tgattaatga tgaagcgctg
    10741 aatcgtatta tcgccgtagg ccaccagcat catctgaacg ttgtcggctg gaacccggga
    10801 ccggcgcttt cagttagcat gggcgacatg ccagatgatg gctacaaaac atttgtttgt
    10861 gtagaaacgg cttacgcttc agaaacgcaa aaagtgacca aagagaaacc tgcacatctg
    10921 gcgcaatcca ttcgcgttgc gaaacgttaa tttgcgttaa tgttgtgtgc cgggtgcgat
    10981 gcatccggca cgtatcaaca tcacaccata tccagcgcag tttttccttt tggtgccgga
    11041 tatgctttat ccagcatgat taattccgct gacgaaagtt cgacctcaag cacagccgca
    11101 ttctgttgga catgggcaat cgtggccgct tttggaatcg ccatcacacc ctgatggctg
    11161 attacccacg ccaacaatac ttgtgccgcg ctgatattgt gagcatgtgc aatttcgttg
    11221 actaccgcgt tttttaacag tccattgcgc aaccgcccgg cctgggctaa tggactgtaa
    11281 gccattaccg gcatctgctg ttgctggcac caggggagta gatcgtactc aatccctcgt
    11341 gaaccgagat ggtaaagcac ctgattagtg gcacactgat ttccccccgg cagctgccag
    11401 agttcctgca tatcagcata atcaaggtta gaaacgcccc agcggcggat ttttccctgg
    11461 gcgatcaatt tttccatcgc tgccacagtc tcttcaaaag cgaaactgcc agaccagtgt
    11521 aataagtaga gatcgagata atcagtattg agacggcgta aactggcttc gcatgcattt
    11581 atcgcttttt gcccgccagc attccacgga tagactttag agacgagaaa gacgttatcg
    11641 cgcagatcgg ttaatgcttc cccaaccacc ttttcggcac cgccatcggc atacatttcg
    11701 gcggtatcaa tgagggttaa accgagctca atgcccgcgc gtagtgcagc aacttctgtt
    11761 ttgcgctgac tggcatcttc gcccatatac catgttccct gccctatggc tggcagtgag
    11821 acatcgccac taaattgaat cattttttgt tgcattgttt cctcccaggt attgcaccac
    11881 cgtaatgcaa aacagggcgt gacgccctgt ttttatgcac aaaatgccct ggaaagatgc
    11941 attatcagaa tttgtaggtg atcccggtag aaatcaggcc agtccaggat ttatccacca
    12001 tcgggctgtc agtaacttca tcagacagac gggtatagcg cgcggtaccg taaacactcc
    12061 agtcgccgag gaagttgtag ctggcgctca gctccaggta agggctccag ctgtcgttcg
    12121 ggttatagcc acgcagaccg ctgcgagcgg actctttgcg cgatacgcca taatagtatt
    12181 cgttctggtt ttcgctgttc cactgcacac caatacccgg agtcacggtc aggccaccgt
    12241 tggtgtaacg atacaaccag gccatatccc agacaatgcc gttgctgtta tccagggtat
    12301 cgccagccaa ggtggtacgc aggtaaccgt actgggtaaa gtgagcataa gacagaccag
    12361 ccatcatggt gctcttacgg tcatccaggt gacgcatttg gtgatcgcca ctgtctttcg
    12421 ctttgaagta aagcggcgac cagtaagcgg taattgaaag tttatccgtt gcgtcattcc
    12481 acaggtagta accaccacct aagccacgga accagaagtt atcgccttca tagttgatta
    12541 ccggtactgg gtaaacatcg gtatcgtaat ctttatatgg gtgctcaacg acacctacgc
    12601 ctgcgcccag ggaaaattta ccttcagcgt gcgctacgcc tgcagacgtt gcgataagca
    12661 ctccaagtgc cagaagtttg agtttggtca caattaatca ttccttaaac aaatgtttag
    12721 cgggcgacaa agtttacccg tcaatacatc gaaacccaac ctttttacgt tttcattttt
    12781 taaagtaact gtttaatttt cctgacgcgg atgacaccgc gcttacagcc aaatgaattt
    12841 agcgttactg gctaccctgg tctttacatt aattatgcaa aatttatgga tgagttgttg
    12901 atatgccatt gaaattaaga aagccgtgca ggcaagtttt ccatttgcca tctacgctta
    12961 attttgaagg tgtatcaccg ggcacgttgt tctcatcgtc gataaaatgg catgagagtt
    13021 gctgtgtttt agcaagagac gtcgttcagt ttacctcttc cgggagcctc tactattcat
    13081 atgaacggct cttaacctgt gctaaaaaac gaaaggacgg cataccatga atatattcga
    13141 tcactatcgc cagcgatatg aagctgccaa ggacgaagag ttcacactgc aggagtttct
    13201 taccacttgt cggcaagatc gcagtgctta tgccaacgcg gctgagcggc tattgatggc
    13261 tatcggtgag cctgtcatgg tcgatacagc ccaggaaccc agactttctc gactcttttc
    13321 taaccgggtc attgcacgtt atccggcatt tgaagagttt tacggcatgg aagacgcgat
    13381 tgaacagatt gtctcttatc tgaaacacgc ggctcagggg ctggaagaga agaaacaaat
    13441 cctgtatctg ctggggcctg tgggtggggg taaatcatcg cttgctgagc gactgaaatc
    13501 attaatgcag ctcgtaccga tttatgtatt gagcgccaac ggtgagcgta gcccggtcaa
    13561 cgatcatccg ttctgtcttt tcaatccgca ggaagatgct cagattctgg aaaaagagta
    13621 tggcattcct cgccgttatc tcggcaccat catgtcgcca tgggcggcaa aacgcctgca
    13681 tgaatttggt ggcgatatca ctaagttccg ggtagtgaag gtctggccgt caattctgca
    13741 acaaattgct atcgccaaaa cggaacccgg tgatgagaac aaccaggaca tctccgcgct
    13801 ggttgggaaa gtcgatattc gtaaactcga acactacgcg cagaatgacc cggacgccta
    13861 cggctattcc ggtgcgctgt gccgcgccaa ccaagggatc atggaatttg tcgagatgtt
    13921 taaagcaccg ataaaagtgc tgcatccgct gttaaccgcc actcaggaag gtaactacaa
    13981 cgggacggaa ggtatctccg ccctgccgtt caacgggatt attctcgccc actcgaacga
    14041 atccgaatgg gtcactttcc gtaataacaa aaacaacgaa gccttcctcg accgtgttta
    14101 catcgtgaag gtgccgtatt gcttacgcat ttccgaagag atcaaaatct acgagaaatt
    14161 gcttaatcac agtgaattga ctcacgcccc ctgcgcccct ggcacgctgg aaacgctgtc
    14221 acgtttttcc attctttcgc gccttaaaga gccagaaaac tccagcattt attcaaagat
    14281 gcgggtttat gatggcgaaa gtctgaaaga caccgatccc aaagccaagt cgtatcagga
    14341 atatcgtgac tacgccggtg tcgatgaagg gatgaacggt ctgtcgacgc gttttgcgtt
    14401 taagatcctc tcccgcgtgt tcaacttcga tcatgtagaa gtggcggcaa acccggtcca
    14461 tctgttctac gtactggaac agcagatcga gcgcgagcag ttcccacaag agcaggcaga
    14521 acgctatctg gagttcctga aaggttatct gatcccgaaa tatgccgagt ttatcggcaa
    14581 agagatccag acggcctacc ttgaatccta ttccgaatat gggcaaaaca ttttcgaccg
    14641 ttatgttacc tacgcggatt tctggattca ggatcaggag tatcgcgatc cggataccgg
    14701 gcagctgttt gaccgcgagt ctcttaacgc cgagctggag aaaatcgaga aaccggcggg
    14761 gatcagtaat ccaaaagatt tccgcaacga gattgttaac ttcgtactgc gcgccagagc
    14821 gaataacagc ggacgcaatc cgaactggac cagctatgaa aaactgcgca cggttatcga
    14881 gaagaaaatg ttctccaata ccgaggagct gttgccggtt atctcgttta acgccaaaac
    14941 gtcaaccgac gagcagaaga aacacgacga ctttgttgac cgtatgatgg aaaaaggcta
    15001 cacccgtaaa caggtgcgtt tactgtgcga atggtatttg cgcgtacgta aatcgtctta
    15061 acaaccctgg cccggtacac atgttaccgg gcctacaacg acagcgaacc gtgggcctga
    15121 gaagcggcaa cacaggcgta gcatacagtt ggcaaatgta gtacgggggg catatgacct
    15181 ggtttattga ccggcgtctg aacggcaaaa acaaaagcat ggtgaatcgc cagcgttttt
    15241 tacgccgtta taaagcgcaa attaaacagt cgatctccga ggccattaat aagcgttcgg
    15301 tgactgacgt cgacagcggc gaatccgtat ccattcccac ggaagatatt agcgaaccga
    15361 tgtttcatca ggggcgtggc ggtctgcgcc accgcgtgca tccgggcaat gaccatttcg
    15421 tccagaacga ccgaattgaa cgtccccagg gtggcggcgg aggttccggc agtggtcagg
    15481 gccaggccag ccaggatggt gaaggtcagg atgaatttgt ctttcagatt tcgaaagatg
    15541 agtatcttga tttgctcttt gaagatttgg ccttaccgaa tctgaaacaa aaccaacaac
    15601 gccagctgac cgaatataaa acgcatcggg cgggttatac cgctaacggc gttccggcaa
    15661 atatcagcgt tgtgcgctcc ttgcagaact cactggcgcg acgcacagcc atgacggcag
    15721 gcaagcggcg ggaacttcat gcactggaag agaatttggc catcatcagc aacagtgaac
    15781 ctgcgcagct gctggaagag gaacgtctgc gcaaagaaat tgcagaatta cgtgccaaaa
    15841 ttgaacgcgt cccttttatt gacaccttcg atttacgtta caagaactac gagaagcggc
    15901 ccgatccctc cagccaggca gtgatgtttt gcctgatgga cgtttccggt tcaatggatc
    15961 aatccactaa agatatggct aagcgttttt atattctgct gtatctgttc ctcagcagaa
    16021 cgtataagaa cgtggaagtc gtatacatcc gccatcatac ccaggcgaaa gaagtcgatg
    16081 aacatgagtt tttctactcg caggaaacag gcggcaccat cgtttccagc gccctgaaac
    16141 tgatggatga ggtagtgaaa gagcgttata acccggcaca gtggaatatt tacgctgcac
    16201 aagcatcgga cggcgataac tgggccgatg actctccgct ttgccatgaa atcctggcga
    16261 aaaaattatt acctgttgtt cgttattaca gctatatcga aattacccgt cgtgcacatc
    16321 agacattgtg gcgagaatat gagcatctgc aatctacttt cgacaacttt gcgatgcagc
    16381 acatccgcga ccaggatgat atttatccgg tgttccgtga actgtttcat aaacaaaatg
    16441 caacagctaa agactaaaac tatcagccag gtcattatcg cctggctgat ttttagctta
    16501 ctgtaaatta tctcatttat tacatacatt agcttacaat cgctttaaat atgacagcat
    16561 aacctttaca taatttagtt ccagaaaaca atcattcgga aaaatgat
//
LOCUS       DAZFQD010000116        16604 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.116, whole genome shotgun sequence.
ACCESSION   DAZFQD010000116 DAZFQD010000000
VERSION     DAZFQD010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16604
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.116"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            47..409
                     /gene="tnpA"
                     /locus_tag="ACHKTW_003805"
                     /pseudo
     CDS             47..409
                     /gene="tnpA"
                     /locus_tag="ACHKTW_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278583.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(508..1176)
                     /gene="ydcL"
                     /locus_tag="ACHKTW_003806"
     CDS             complement(508..1176)
                     /gene="ydcL"
                     /locus_tag="ACHKTW_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HFO6464743.1"
                     /translation="MRTTSFAKVAALCGLLALSGCASKITQPDKYSGFLNNYSDLKET
                     TSATGKPVLRWVDPSFDQSKYDSIVWNPITYYPVPKPSTQVGQKVLDKILNYTNTEMK
                     EAIAQRKPLVTTAGPRSLIFRGAITGVDTSKEGLQFYEVVPVALVVAGTQMATGHRTM
                     DTRLYFEGELIDAATNKPVIKVVRQGEGKDLNNESTPMAFENIKQVIDDMATDATMFD
                     VNKK"
     gene            complement(1479..2072)
                     /gene="tehB"
                     /locus_tag="ACHKTW_003807"
     CDS             complement(1479..2072)
                     /gene="tehB"
                     /locus_tag="ACHKTW_003807"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0046690 - response to tellurium ion
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HFO6464744.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDRQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFPFAFKEGELRRYY
                     EGWERVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
     gene            complement(2069..3061)
                     /gene="tehA"
                     /locus_tag="ACHKTW_003808"
     CDS             complement(2069..3061)
                     /gene="tehA"
                     /locus_tag="ACHKTW_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HFO6464745.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCLFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSENGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            3185..4165
                     /gene="ydcK"
                     /locus_tag="ACHKTW_003809"
     CDS             3185..4165
                     /gene="ydcK"
                     /locus_tag="ACHKTW_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcK family protein"
                     /protein_id="HFO6464746.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKNVLLRQIIAISDFNDVIAGTAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGAVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDSVTIHDSLVCGQCRIFGHALINQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVIRYAFIEHRAEVFDFASIEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ITGAVIIENHVELTDHALVEAFDGDTVHVRGPKVINGEGRITRTPLAGLL"
     gene            complement(4160..4696)
                     /gene="rimL"
                     /locus_tag="ACHKTW_003810"
     CDS             complement(4160..4696)
                     /gene="rimL"
                     /locus_tag="ACHKTW_003810"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HFO6464747.1"
                     /translation="MTETIKVSESLELHAVAENHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTVEIGYWLDE
                     SHQGQGIISQALQALIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDS"
     gene            complement(4759..4983)
                     /gene="ydcH"
                     /locus_tag="ACHKTW_003811"
     CDS             complement(4759..4983)
                     /gene="ydcH"
                     /locus_tag="ACHKTW_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HFO6464748.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKEV"
     gene            complement(5123..6778)
                     /gene="opgD"
                     /locus_tag="ACHKTW_003812"
                     /gene_synonym="mdoD"
     CDS             complement(5123..6778)
                     /gene="opgD"
                     /locus_tag="ACHKTW_003812"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HFO6464749.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGIAP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            complement(7003..8346)
                     /gene="ydcJ"
                     /locus_tag="ACHKTW_003813"
                     /pseudo
     CDS             complement(7003..8346)
                     /gene="ydcJ"
                     /locus_tag="ACHKTW_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310055.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            8563..9486
                     /gene="ydcI"
                     /locus_tag="ACHKTW_003814"
     CDS             8563..9486
                     /gene="ydcI"
                     /locus_tag="ACHKTW_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFO6464750.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSK
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEDLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSASLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            complement(9524..11164)
                     /gene="trg"
                     /locus_tag="ACHKTW_003815"
     CDS             complement(9524..11164)
                     /gene="trg"
                     /locus_tag="ACHKTW_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HFO6464751.1"
                     /translation="MNTTPSHRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASRIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEALDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRPLDNAYTDILNKAVKIRSTRANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMKDEPAGRNEIGRLNRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHHA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVDVFRLNKHSVSAEPRGAGEPVSFATV"
     gene            11563..11712
                     /gene="hokB"
                     /locus_tag="ACHKTW_003816"
     CDS             11563..11712
                     /gene="hokB"
                     /locus_tag="ACHKTW_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HFO6464752.1"
                     /translation="MKHNPLVVCLLIICITILTFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
     gene            complement(11690..11758)
                     /gene="yncP"
                     /locus_tag="ACHKTW_003817"
     CDS             complement(11690..11758)
                     /gene="yncP"
                     /locus_tag="ACHKTW_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YncP"
                     /protein_id="HFO6464753.1"
                     /translation="MNLLVRCAGKIPALALTWTCRP"
     gene            complement(11784..11957)
                     /locus_tag="ACHKTW_003818"
     CDS             complement(11784..11957)
                     /locus_tag="ACHKTW_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6464754.1"
                     /translation="MKKLALILFMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
     gene            complement(12202..12732)
                     /gene="cybB"
                     /locus_tag="ACHKTW_003819"
     CDS             complement(12202..12732)
                     /gene="cybB"
                     /locus_tag="ACHKTW_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HFO6464755.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMV
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            12921..13922
                     /gene="gap"
                     /locus_tag="ACHKTW_003820"
     CDS             12921..13922
                     /gene="gap"
                     /locus_tag="ACHKTW_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFO6464756.1"
                     /translation="MSKVGINGFGRIGRLVLRRLLEVKSNIDVVAINDLTSPKILAYL
                     LKHDSNYGPFPWSVDFTEDSLIVDGKSISVYAEKEAKNIPWKAKGAEIIVECTGFYTS
                     AEKSQAHLDAGAKKVLISAPAGEMKTIVYNVNDDTLDGNDTIVSVASCTTNCLAPMAK
                     ALHDSFGIEVGTMTTIHAYTGTQSLVDGPRGKDLRASRAAAENIIPHTTGAAKAIGLV
                     IPELSGKLKGHAQRVPVKTGSVTELVSILGKKVTAEEVNNALKQATTNNESFGYTDEE
                     IVSSDIIGSHFGSVFDATQTEITAVGDLQLVKTVAWYDNEYGFVTQLIRTLEKFAKL"
     gene            complement(13964..15403)
                     /gene="aldA"
                     /locus_tag="ACHKTW_003821"
     CDS             complement(13964..15403)
                     /gene="aldA"
                     /locus_tag="ACHKTW_003821"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HFO6464757.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAE
                     DARKAIDAAERAQPEWEALPAIERASWLRKISAGIRERASEISALIVEEGGKIQQLAE
                     VEVAFTADYIDYMAEWARRYEGEIIQSDRPGENILLFKRALGVTTGILPWNFPFFLIA
                     RKMAPALLTGNTIVIKPSEFTPNNAIAFAKIVDEIGLPRGVFNLVLGRGETVGQELAG
                     NPKVAMVSMTGSVSAGEKIMATAAKNITKVCLELGGKAPAIVMDDADLELAVKAIVDS
                     RVINSGQVCNCAERVYVQKGIYDQFVNRLGEAMQAVQFGNPAERNDIAMGPLINAAAL
                     ERVEQKVARAVEEGARVALGGKAVEGKGYYYPPTLLLDVRQEMSIMHEETFGPVLPVV
                     AFDTLEEAISMANDSDYGLTSSIYTQNLNVAMKAIKGLKFGETYINRENFEAMQGFHA
                     GWRKSGIGGADGKHGLHEYLQTQVVYLQS"
     gene            complement(15600..16400)
                     /gene="ydcF"
                     /locus_tag="ACHKTW_003822"
     CDS             complement(15600..16400)
                     /gene="ydcF"
                     /locus_tag="ACHKTW_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF218 domain-containing protein YdcF"
                     /protein_id="HFO6464758.1"
                     /translation="MNITPFPTLSPATIDAINVIGQWLAQDDFSGEVPYQADCVILAG
                     NAVMPTIDAACKIARDQQIPLLISGGIGHSTTFLYSAIAQHPHYNTIRTTGRAEATIL
                     ADIAHQFWHIPHEKIWIEDQSTNCGENARFSIALLNQAVERVHTAIVVQEPTMQRRTM
                     ATFRRMTGDNPDAPRWLSYPGFVPQLGNNADSVIFINQLQGLWPVERYLSLLTGELPR
                     LRDDSDGYGPRGRDFIVHVDFPAEVIHAWQTLKHDAVLIEAMESRSLR"
BASE COUNT         4307 a         4163 c         3862 g         4272 t
ORIGIN      
        1 tgaataaaga aaccgatatc cgacggggaa gcattgtgtt ttcctgatgc atgttcacct
       61 ggtctttgtc accagatacc gacgtcagat ttttgactat gacgcgacag aaaaactacg
      121 cacttacttt tcaaatgtat gtgctgattt tgaagctgaa ctggtttaaa tggatggcga
      181 accagatcac gtccatttgt taattaacta tcctcccaaa ctggcgatat ccagtctggt
      241 aaacagcctc aaaggcgtat cgagtaggtt actgcgacga gatcgaccag atattgcagt
      301 caggtattac tacaaaggcg ttttgtggag tcctggctat tttgccagta gctgcggagg
      361 tgcgccaata tccgtcatcc gccaatacat tgaacaacag caaacacctg gtcaggtgga
      421 aaaccgcgcc ttatatcccc gccctgaagg atggggtttt acggcgcacc ggataaaaag
      481 cgccatctaa cgatggcgcg tctggaacta ctttttgtta acgtcaaaca tggtggcatc
      541 ggtcgccatg tcatcaataa cttgtttaat attttcgaaa gccattggcg tactttcgtt
      601 attcaggtct ttaccttcgc cctgacgaac gactttgata accggtttat tagtcgctgc
      661 atcaatcagc tcaccttcaa aatagaggcg agtatccatg gtacggtggc ctgtagccat
      721 ttgcgtcccc gcaaccacta atgcaacagg aaccacttca tagaattgca gcccttcttt
      781 gctggtatct acaccggtaa tggccccacg gaaaatcaga ctacgcggcc cagcggtggt
      841 aaccagtggt ttacgctgcg ctatcgcttc tttcatttcg gtgttggtat agttcaaaat
      901 tttatccaga actttctgcc ctacctgggt agaaggtttt ggtaccggat aataagtgat
      961 tgggttccag acgatgctgt catatttgct ttgatcaaaa ctcgggtcta cccaacgtaa
     1021 aacaggttta cctgttgctg aggttgtttc ttttaaatca gagtaattgt ttaaaaaacc
     1081 agaatattta tctggctggg tgattttaga ggcacaacca gacagagcca acaagccgca
     1141 taaagctgca actttcgcaa atgatgtggt acgcatgaat agtttcctga gatgattata
     1201 ttactttcta agttatagca aaaagcgccg cttcgtgttg tggcaaaaaa agtgttggtg
     1261 aaaataaatt tttcagcccc ttttcagacg acatagtcaa tgccgaaaaa ggggctaagt
     1321 caatcatcag tagttaaatt tcagtaagtt aagctgctaa aaaacttcga ctactgtgat
     1381 taatttcaca tcccttcgat aaaaaatatg cttaccgaaa agaagaacag taaatcatct
     1441 cagcgtgggg aattttcacg cgccttattc ttagcgggtc attttttacg tgccagcatc
     1501 gtggcgaaac gcagtttaat acgattaccg ttggcgtcgg tgcggtgcag ctcgccgacg
     1561 tcttcattgt atttcaccct ctcccagcct tcgtaatatc gacgtaattc tccctctttg
     1621 aaggcaaacg ggaagccgac ggtacatgga taatcagcgg tatccatcgc cgccacaatc
     1681 aggttgtaac caccaggttt agtgcaacgt tgcatattgg caatcagccc tgggatggtt
     1741 ttagcctcaa ggaacatcag taccacagtc gaaagaataa aatcgtactg tctatcaaat
     1801 gtgaggttat tcagatcaac gactcgggtg tgtaaattat ccagattttc aatggattta
     1861 atgcgctcga cgttggcgat actcatggca tttttatccc atgcgtcaac atcataacca
     1921 ttggctgcca ggtaaagact gttacgacca ttgccacagc ccagatccag cgttttaccc
     1981 ggtttaacca ctttcaccgc ttccagtact tcagagtgtg tgcgggttaa ttcatattta
     2041 tcagtaaaat agttttcgtc acgaatgatc attctttgtc ctctgctttc attaaaacgg
     2101 cgcgctcggt tctgactaac aattttccct gcatcagaag cgcaaaagta cggatgagca
     2161 gtattgcaat aataaaattg gtaaagataa acagcggtac cgccagcgta tggaaaaatc
     2221 cattttcgct gccactcccc agatgcaaac cggtggttgc cagtgcagat acgccgaacg
     2281 agaaactcca gaatgaagca ttaaatggct gggagagata ccatggcatc agacgtagca
     2341 taaacagcag ttgcagcagt ccataaccaa aaagcatttt cgccagcgta tcaccctcgc
     2401 cgccgttgac gctcaaccag gcactacagg ccaccagcgc aggagcgagc tgaatgccga
     2461 gtgatgtccg cagtgccgtg ggtaattctc ccgaactgcg cagacgctgc aagatcacag
     2521 gttcaaggct tagccatgag aaaacgcctg cgcctaaaaa caccagaccg gcgtcggtgt
     2581 agcccaacgc accacaggcc attgcgctga taaagttgtt ggcaactgtc ggcagataca
     2641 gtccaggcgt ggtagcttct tcagggtgag atccgcgcca taatcccgca gtttgccagg
     2701 cggcataagc caactgaacc acgacaccaa aactgaacag gcacaccgcc agtgggcgaa
     2761 accacggaac aaaaccaatc gccaccagca tcgtcgttgc cggaaacaaa ctcacaaaac
     2821 tgctcagcac tggatggcgc acttctgcca gcacgctatg cggaaagcgt ataagtcggg
     2881 taataaatgc gctagttaac aatccccaga tgatcatcgc cagaatcacc agcccatccc
     2941 ctaaccagtg gctgacctgc caaacctggc tggcatagcg ccaggcaaat cccatcccta
     3001 tcgtccccaa cacaatacca aagtagcctg ccggcaaatt gagcacttta tcgctctgca
     3061 tcttcttact cattttgttt aatttataaa gtatatttga aatgcatttt atggaatttt
     3121 ctcgagcata gctagagccg cagaatttgc tacggttata gtaagtcacc tgtgggaaaa
     3181 cgctatgcgc aaatatcgtc taagtgaaga acagcgagcc tttagttatc aggaagatgg
     3241 cactaaaaaa aatgtgttat tacggcagat tatcgccatt agcgatttta acgatgtgat
     3301 agcaggaacc gctggcggct ggatcgatcg cgaaacggtg ttagcacagg aaggaaattg
     3361 ctggatttac gaccagaacg ctattgcgtt cggcggagcg gtgatttccg gtaatacgcg
     3421 catcaccggc accagcgtgc tgtggggaga ggtttacgca acggataatg tctggatcga
     3481 taatagtgaa atcagccagg gtgcatatat aagtgacagc gtcaccattc atgactcact
     3541 ggtttgcggg caatgtagaa tatttggtca cgccttaatt aatcaacatt ctatgatcgt
     3601 cgcagcacaa ggcttaacgc ctgaccatca actcctgcta caaatttatg atcgcgcaag
     3661 ggttagcgcc tcgcgtattg tccaccaggc acaaatttat ggcgatgccg tcatccggta
     3721 tgcttttatt gaacatcgtg ccgaagtttt tgattttgcc agtattgaag gtaatgaaga
     3781 gaacaacgtc tggctatgcg attgcgctaa agtctacggt cacgcgcagg tgaaagctgg
     3841 catagaagaa gatgccattc caacgattca ttacagttcg caggtggcgg aatatgccat
     3901 tgtggaaggt aattgtgtgt taaaacatca tgtcttgatt ggcggaaatg ccgtggtacg
     3961 tggtggaccg attctgctcg acgagcacgt tgtaatacaa ggtgagagcc gtattacagg
     4021 tgcggtgata attgaaaacc atgtggagtt aaccgaccac gccctcgttg aagcctttga
     4081 tggcgacacg gttcatgtcc gtggtccaaa agtcattaac ggcgaggggc gcattacgcg
     4141 aacgccgctg gcagggttat tatgaatcga taatacgcgc gtataagttc acatcatcat
     4201 aggcatcatt caggaactca gcctgtttca ggcaaccttc aaggataaaa ccattgcgca
     4261 aagcgacctg gttgctttgc ggattgtcca cccgacattt gatcacgaag cgtctaagtt
     4321 caccagactg ggcgtaatga tgaatcaatg cctgcagcgc ctgagaaatg atcccctgcc
     4381 cctgatgaga ttcgtccagc cagtagccta tttcaacggt tttattcagt ggttcaatac
     4441 gattaaacga gataacgccg ataagttcat cttctttgaa aatcatgaac attttggcat
     4501 agccgcgttg atgcaacatc acattaccct gcaccgtttt tcgcgtgtcc tcttcactct
     4561 gaacgaactg cggccagttt agcgactgct gtaaccaggt tttattttta cagattaact
     4621 gataaagagg agtgacgtga ttttctgcaa cagcatgtaa ttcaagtgat tcgcttactt
     4681 ttatcgtttc agtcatctta tctccagtca atccatgcag ttgttaaaaa agccgcctgg
     4741 ctggcggctt aggaaagttt atacctcttt gacgctctcc tgctgcagga ttttgagcat
     4801 ctcatctttt aactgtagct tttgtttttt catgcggacc acttccgcat tgtaccctcg
     4861 accgtcggaa ccttcctttc tggcaatttc atgatcaagt ttattgtgtt tatcgaacaa
     4921 ggacataaag cgaggatttt cgtttttcag acgggatatt aaatctcgat attctggaaa
     4981 catacgcact ccctgttagt ggctgacaag gtgtatctga aatacacacc atcagattac
     5041 tcatttaact tagggaaaaa tgcgagcggg atcgtgttta accagacggc acctcccgaa
     5101 gaaggtgccg aagaaaaaac gattaactca tcacgcggtc gtcaacatac tgacgtttat
     5161 ccggcgctgg cgggaaatac tgatacagcc atgtttcact gatagcgtcg ccctgacaac
     5221 gcagatacat ccgcatatcg accggatcag tggagtccga agtcggatac cagtcaaact
     5281 gaatacgata accatcaatg ggttcaatat agagaatttc gatttgcttc gcttccccac
     5341 tggaaagcgt aatcaccggc tcaatgcctt ttggtgcggc agctttcaga tcaccaccaa
     5401 cgaaatcgac ggcaaaacga cgcgcccatt tttcgggata gtgttcacct ggtgcccaac
     5461 cttccgggaa accgcccatg ccggtacgcg tcgccataac gcgcgctaat gggcaatgaa
     5521 caggcggttg cgcactccag tacagacgat actggaatgc aaactcatca cccgccttta
     5581 cagctttttc tggctgccag aagcagacaa tgttatccag cgtttcgccc gttgttggga
     5641 tttccatcag gccgatggtg cccttacccc acttgttacg cggttccacc cacagacttg
     5701 ggcgtttgtt ataccagccc ataatgtcct gataatggga gaaatcacga tccagttgca
     5761 ataaaccaaa ccctttcggg ttgttgtctg tgtaagcatt gaactgcaat ttttgcggat
     5821 tattcagcgg acggcaaatc cactcgccgt tgccccgcca catggacaga cgatcagagt
     5881 catgaatttg cggatgaatg gtgtcgcaca tccgacgttc attagtaccg cagctgaaca
     5941 tactggtcat cggcgcaatg cccagctgtt taatgtcttt gcgcgcatac aggtgatttt
     6001 ccacatccat aatcacctga cttttctcac aatggatagt gaacttatag gcaccagtaa
     6061 tgctggcgct atcgagcaac gcataaacgg taaaggtagt tgcccccggt tttaccgtat
     6121 caaaccagaa ggcggtaaag tcggggaact cttctttact gtcggtgtaa gtgtcaatcg
     6181 ccaggccgcg ggccgacaaa ccgtattgat atgtgtcatc aacggcgcgg aaataactcg
     6241 ctccgaggaa tgatactaca tcacggcgcg ccagttcggg ggctttaaac acgcgaaaac
     6301 cggcaaagcc gagatcgctt tgcccttcta attgtttggt atcaacacct gcatcgttgt
     6361 atttgaacaa ctccgggcga aagtgaattt cacgcgccag atgtgttgct ggatctacag
     6421 aaaacatacg aacgcggcga cggaatccca ttcccatatg gaagaactga gcgtccagtt
     6481 gacggttctc aacgttatgc cagagcgatt tttcggcgtc gtattgaata ctgttataag
     6541 cctgcggcgt cattgtcgcc agcgtgtcag gtaacggacg aggcgcacca cgccacgctg
     6601 tttgcgctaa gtcgtgcgcc attgactgta gaatggagaa gtcaaaacgc tgggtttgcc
     6661 cgtcggcaat atcagaatct gccgcgaatg ccgcctgaga aaaaagagaa gcaatgccgc
     6721 tggtaccgca cacggcggcc atagccattg aacctttaat aaatcgtcta cgatccatac
     6781 ctgaaagtga gtccttctgg tgttgtgaat ggtcccgcgc tggtcgtttt cagcggcaag
     6841 aaagtgctta tttatgaccg ctcactctag acaatattta ttaataatcc aattgttgaa
     6901 ctcagagaat atgcataaat cggcaatttt tttatgtcga tgggatgagc ctgattcgtt
     6961 tgtaaaatca ggcaaatttt tgcaaaggcg gatggtcaga atttaaagca aaccacagcg
     7021 acgtttactg cgttcttccg cttcctgata aagctggaac tcatccaaaa ccggacaacc
     7081 caacgcctgc tcaaatgcct cgcgactggc attaccgtga ttgcgtgcct gcgtttcatt
     7141 acccagattt gactggaaga tccccgccgc gctaacgggc aagaaatctt cataggtaat
     7201 gggttgcgcc gcgacccaac cacgttcaat taagggctgt ggatcgtctc cgggatgaat
     7261 cgcctgacga tgcgcctcac ctgaaggcgt cagacggtac cggaaccatg ccagcccttg
     7321 ctgacgcatt aaaaactcac tgtcaggaaa agtgcggaag gtttcctgta aatgcatttg
     7381 gtgagtgaga ttatcctgcc cggttccagc gttacgcaga agatcatcat acagttgtcg
     7441 ccctttcggc gttaatgcca cgccacgctg ctcaatttca ccaaagcgcg cggtatgcgt
     7501 gccctgtttc tgccccgcaa acaacaccgt ctcttccagt gctttaaagc tggtctggcg
     7561 tagtaaaatc ggtacctcgc ggcgcggcgg cccctcgatg agaattttgg gttcaattcc
     7621 gcattcaggc atcatcgact gcacccggtc aatatccagc gtacgtggcg tcaggtggtt
     7681 gatatggcat ccaggaaaac agaccacatc agcaatcaac cgatgttcgt tgtgcaaggc
     7741 gcgataggtt tcttcatcta ccgttgctga ctggtgccag cgaaacgttt ccagggcttc
     7801 ctgcacaaac tcctgtgcct gtgtttcgtt aaaaccgccc tgctactcat attcctctaa
     7861 cagttgtcga caacgtgggg tgaagatatc gcgctgacgc agaatctccg ccgctttctg
     7921 gcgcaaaatt tcgttctcga taagctcaag gcggagtaag gaggtaaaaa cgcggaaggg
     7981 attacgcgcc agagaagcat catcaatggg ccgaaatgct gtcgaatgta ccggcacccc
     8041 tgcctgcgag agatcgtaat agctcaccgg gtacatcccc ataatggcaa acatccgccg
     8101 aagagtagca agctcttgtg cagtcccaac gcgaatcgcc ccatgacgtt caacattcag
     8161 tcgcgccagc tcgtctgcat ttaccatttt ttcgtgcagt tgaggattgt tttccagcac
     8221 agccagattc acatcagcta ccagttccag cagcgtgcca tattgcggaa cttcttgctg
     8281 gtacatggct gacattgcct gcgaaaactg ttcccgaatc tcatccgccg tgatgctgtt
     8341 cgccatagtg tctgcctcca gtggtcagta atctggagtg taggtaaccg cattcactct
     8401 ggcgggaaga atttacaaac tgtgatctcg ccgcgaaaac atcaatatta tccattttgc
     8461 tgtaacataa ttgctttaat tgttaataat attttgcaat caagttatca taatcaaaca
     8521 acttcacttg tcagcgacac cgcttcgttt ttaacatcgc ttatggaaaa aaatagtctg
     8581 tttagtcagc gcatccgttt gcgccacctt catacattcg tagctgtcgc acaacaagga
     8641 actttggggc gcgcggctga aacccttaat ttgagtcaac ctgcgctctc taagacattg
     8701 aatgaactgg agcagctgac tggcgctcgc ttgtttgagc gtggtcgtca gggggcgcaa
     8761 cttaccttac ccggcgaaca atttttaacg catgcagtca gagttcttga cgccatcaac
     8821 actgccggac agtcgcttca tcgtaaagaa gatcttaata atgatgtcgt cagggttggt
     8881 gcactaccta ctgcggcact ggggatatta ccttcggtta taggtcagtt tcatcagcaa
     8941 caaaaagaga cgaccttgca agttgcgaca atgagtaacc ctatgattct ggcgggttta
     9001 aaaaccgggg aaatcgatat cggcattggt cggatgtcag atcctgaact gatgaccggg
     9061 cttaattacg aactgctgtt tcttgaatcg ctgaagctgg ttgtccgccc taatcacccg
     9121 ctacttcagg agaacgtaac gctaagccgg gtgctggaat ggccggtcgt tgtatcacca
     9181 gaaggcactg cgccacgcca gcattcagat gcattagtgc agagccaggg ctgtaaaatt
     9241 ccttcgggtt gtatcgaaac gctgtctgct tcgctatctc gtcaacttac ggttgaatac
     9301 gactacgtgt ggtttgtccc ttctggcgcg gtaaaagacg acctgcgtca tgccacgctg
     9361 gtggccctgc ctgttccggg acatggtgca ggcgaaccga ttggaatact gacccgcgta
     9421 gatgcgacgt tctcttctgg ttgccagttg atgattaacg ctattcgaaa atcaatgccg
     9481 ttctgaaagg tgaagggatc tgtcgatccc tccttgaaca ttttcacacc gtagcgaaac
     9541 taactggttc acccgctccg cgaggttctg ccgacacaga atgtttgttc agacggaata
     9601 cgtccaccgc ctcagttaat cgtgccgcct gttcttcaag ggacaccgcc gctgctgagg
     9661 cctcttctac cagagaagca ttctgttgcg tcaccttatc catttcagaa atcgcctggc
     9721 taacctgcgt tatgcctcta ctttgttcat ccgaggcggc ggcgatttcc tgcatgatat
     9781 gtgtgacact cgcgacggca tcaacaatag tgctcatggt tttccctgcc gttgccacct
     9841 catccgaccc caggtcaatt aacctgactg attcactgat caagccttca atctctttcg
     9901 ccgcttgggc gctgcggctt gcgagtgtcc gtacttcgct ggcgacaacg gcaaatccac
     9961 ggccttgctc acccgctcgc gcggcttcaa cggcagcatt cagtgccaga atattcgtct
    10021 ggaaagcaat actgttgatg acggcggtga tctcagaaat tttcttcgaa ctcgtggaga
    10081 tagcgcccat cgtttttact acaccggaaa ccgtctgccc gccatcgctg gctttaatag
    10141 aagcctcttg cgccagtttg ctggcatgat gcgcgttatc cgcattctgt ttcaccgtcg
    10201 cagtgagttg ctccatgctg gcggcagttt gttcgattgc cgccgcttgt tcttcggtgc
    10261 gagatgacag gtccgcattg ccagctgaaa tttcgctggt gccacgataa atctcttccg
    10321 caccctgtcg aacagtccct actgtcatcc ccagtgaatg ctgcatttgc tgtaaatgac
    10381 gatttaagcg accgatttca ttacgacccg ccggttcatc cttcatcgtc agatcgccac
    10441 tagcgatttt ttcaatccgt tgtgcggcat gttgcagtgg acgaatgacg atccgacgta
    10501 gcaccataaa tgttatcagc gtcatgacca gggcaagcac aaacgcgcca atcatgaaca
    10561 tcccacccag gcgggtgcgc tgatgggcca gttccgccag ttggttggcc ctggtgctac
    10621 gtatcttaac ggctttgttc aaaatatcgg tataagcatt atccagcggt cggatctgct
    10681 cactttcatg attaataatc gcttcaaaca tgccattttt ggcatatttc aacataggtt
    10741 gcatacccgt gatataagcc tgaaagcgtt gatttaattc agtgtcgagg gcttcatcag
    10801 caggtgtttt caccggtcga ttctgataag cacgataacc ttgctgcgac tgtttaatct
    10861 ccgattcggc ttgcgcaata ttgcgcttca ttgcttccat ttccgcaata cgactcgcag
    10921 ccccggcctg aatcatattg atccgggctg aacgcaaaaa atctgaactg ttcgctaacc
    10981 cggtccgaat ctcaatctcc gcagtaacat cacgctgatc gcgatcggcc tgccagagga
    11041 aatagccagc cagagcagaa cttaatgcga atagaactaa gataccgcca agaatgcagg
    11101 caaataacgg aaccagcctg atgtgatgca aaaaacctaa tcgatgtgag ggagttgtat
    11161 tcattaccgt tgtctctcgt ccaggtttac tcctgataga aagtcatcgg cacttgacgg
    11221 taattactta tgaccaaaaa cctctttgcg tcacattttt acaacaatta ttgcagagaa
    11281 aattaatgct attggaatga agagagtaac gaatggctaa actggctaac ccgaatgacg
    11341 aaaaattctg gaaaacagca cgcaatttcg tgaagtgatt attttcgtcc ctaaggaaac
    11401 ctcataggat gcctgcgggt gcctgagact ttctgttttc aggctaaccg ggaatcagaa
    11461 agacgaaggc cccgaccgat atttctatca accgaggcca acgaatgaac ctagcaattc
    11521 agatcttagc ctcttacccg ccatccggca aggagaaagg ctatgaagca caaccctctg
    11581 gtggtgtgtc tgctcattat ctgcattacg attctgacat tcacactcct gacccgacaa
    11641 acgctctacg aactgcggtt ccgggacggt gataaggagg ttgctgcgct catggcctgc
    11701 acgtccaggt aagggcaagc gcggggattt tccccgcgca tctgactagc agattcattt
    11761 tctcatggca ccccttttac acttcagtta gtgcatgttt ttttcgaggc actaatagaa
    11821 ccatcattac agacaaattt gccgcctgcc gtacagtgtg agatcccccc tttcgaacca
    11881 gaacagggtt tgcgcccggc atcggcataa aaggaaacaa gcgttcccat aaacaaaata
    11941 agtgccagtt ttttcatata acctccatgg tttatcgaat cttatgttcg atttgaacgt
    12001 tatacgctgg tttattttga taccgttgat gagatcaaag atcgggtgaa ttgattaaaa
    12061 ataagccaga tggacgtatg ggcaaggatt atggttttat ttgtcataca aataaatata
    12121 ataggcgctt ccgatgtaga cccgtattct tcgcctgtac cacgggtcgg ttttagtaca
    12181 ggcgttttct ttaaatatcc ttcaggaacg tttacgcggc atcatgcgta gaagtgtgtt
    12241 gtccttccag aaatagtggt gtgccagtgc agccgcagcg tgcaacccga tgacaaaata
    12301 tcccagattc gccagcgtct catgccacga ctttaagcta tcaacccgtt cgaaattggc
    12361 ctctgaagcg taaggcatcg tcaaaccaaa cgcaaaccac gggttgcccc ggttatacat
    12421 catcaccaaa ccaatcactg gcagcgcaat aaacaggaga taaatcacca aatgtcccaa
    12481 atgcgccagt cccgtcatca tcggttttgg cttaggtata atcggcgggg ttgggtattt
    12541 cagtcttaac aaaagacgaa ccaccatcag cacgagaatt gagataccac aggaaacatg
    12601 aaccatgttg atgagtggcc gatcgctacg tgggaagaaa ccacgaaact ccattgcgca
    12661 atacgctgcg ataaccagca aaaagaccag ccagtgaatg ctgatttgta accttgagta
    12721 tttattttcc ataacatttc ctgctttaac ataattttcc gttaacataa cgggcttttc
    12781 tcaaaatttc attaaatatt gttcacccgt tttcagtacg ttacgtttca ctgtaagaat
    12841 ggagattgcc catcccacaa tcctgggcta agcctggaaa ggatcaattt tcatccgaac
    12901 gttcctgaca ggagaaaacc atgagtaaag ttggtattaa cggttttggt cgtatcggtc
    12961 gactggtgtt gcgtcgatta cttgaagtca aaagcaacat agacgttgtc gctattaatg
    13021 atctcacttc cccaaaaatt ctcgcctatc tgctgaaaca tgattcaaac tatggtccgt
    13081 tcccctggag cgttgatttt acggaagatt cacttatcgt tgatgggaaa agtatctcgg
    13141 tttacgccga aaaagaggct aaaaatattc cgtggaaagc gaaaggggca gaaatcattg
    13201 tcgaatgtac tggcttttat acctccgccg agaaatcgca ggcgcatctt gatgctggtg
    13261 cgaagaaggt gttgatttcc gcccctgccg gtgaaatgaa aactatcgtt tataacgtca
    13321 atgacgacac tctggatggc aacgacacca ttgtttccgt ggcgtcatgc accactaact
    13381 gtcttgcgcc gatggccaaa gccttgcatg acagtttcgg gatagaagtc ggcacgatga
    13441 cgaccattca tgcctatact ggcacccagt cactggtgga tggcccacgt ggtaaagatt
    13501 tacgtgcttc acgcgcagcg gcagaaaata tcattcccca cactacgggg gcggcaaaag
    13561 ccattggtct ggtgatcccg gaactgagcg gcaaactgaa aggtcatgcg caacgcgtgc
    13621 cggtgaaaac aggttcggtc actgaactgg tatcgattct cggaaaaaaa gtgactgccg
    13681 aagaggtgaa taacgcactt aaacaagcaa ccaccaataa cgagtcattt ggttataccg
    13741 atgaagaaat agtctcttcc gatatcattg gcagccattt cggttcggtg tttgatgcca
    13801 cgcaaacgga aattaccgcc gtgggcgatt tacaactggt gaaaacggtc gcctggtacg
    13861 ataacgaata tggcttcgtc acgcagctta ttcgcaccct cgaaaaattc gctaaactgt
    13921 gacgcgcaca ggcggaggaa aaaacctccg cctcttttac tcattacgac tgtaaataaa
    13981 ccacctgggt ctgcagatat tcatgcaggc catgtttacc atctgcgccg ccaataccgg
    14041 atttacgcca tccggcgtgg aagccttgca tagcttcgaa gttttcacgg ttgatgtaag
    14101 tttcaccaaa cttcagccct ttaatggctt tcatcgcgac gttcagattt tgggtataga
    14161 ttgatgaggt caggccgtaa tcactgtcat tagccattga gatagcctct tccagcgtgt
    14221 caaatgcgac caccggcaga accgggccaa aggtttcctc atgcataatc gacatttcct
    14281 ggcgaacatc cagcagcaat gtcggcggat aataatatcc tttcccctct accgctttgc
    14341 cacccaacgc cactctcgcc ccttcttcta ctgcgcgcgc cactttttgc tcaacccttt
    14401 ccagcgccgc ggcgttaatc aacggcccca tcgcaatgtc gttgcgttca gcggggttac
    14461 caaattgaac cgcctgcatc gcttcaccca gccgattgac gaactgatca taaatgcctt
    14521 tctgtacata aacacgttct gcacagttac acacttgccc actattaatg acgcgtgaat
    14581 caacgatggc tttgactgcc agttcaagat cggcatcgtc cattacgata gctggtgctt
    14641 taccccccaa ttccagacac actttggtga tgtttttcgc cgcagtcgcc atgatcttct
    14701 cacctgcaga gacgctgcct gtcatactga ccattgcgac ctttgggtta cccgccagtt
    14761 cttgcccaac ggtttcacca cgccccagta caaggttaaa cacgccgcgc ggaaggccta
    14821 tttcatcgac gattttggcg aatgcaatcg cattgtttgg cgtaaattca ctaggtttaa
    14881 tgacgatggt attaccggtc aaaagagcgg gagccatttt gcgggcaatg aggaagaacg
    14941 ggaagttcca cggcagaatg ccggtagtca caccaagcgc acgtttaaac aaaagaatat
    15001 tttctcctgg acgatcgctt tgaataatct cgccctcgta acgccgtgcc cactccgcca
    15061 tgtaatcgat atagtcggca gtaaaagcca cttcgacttc agccagctgc tggatcttgc
    15121 ccccttcttc aacaatcagc gcactgattt cactggcgcg ttcgcggatc ccggcggaga
    15181 ttttgcgcaa ccaactggcg cgttcaatag caggcaacgc ttcccattct ggttgtgcac
    15241 gttctgctgc atcgattgcc ttacgggcat cctcggcctg accatcgggt atgcgggaaa
    15301 tgacagcctc tgtagcaggg tttaccacat caatccatgc gtctccacgc caggtaacaa
    15361 actgtccatc gatatacata ggatgttgaa cgggtactga catgggcgac tcctgtgatt
    15421 tatatgtttg ttttcggtga atgcattgtt aattaacatc aatcatcaga gagacggcat
    15481 cgtgaaagga caattctgtg aagggcttca taaaaacaag tgtaaaagga ataaaaggca
    15541 gaaataaaag tcaggagtca tgcccgtttg caaacaagtg gttcacaaac gggcaatttt
    15601 taacgtaacg agcgactttc catcgcctcg atgagcaccg catcatgttt cagcgtttgc
    15661 catgcatgga tgacttctgc cggaaaatca acgtgaacga taaaatctcg cccgcgggga
    15721 ccgtagccat cgctatcatc gcgtaaacgc ggcagctcgc cagtgagtag tgagagataa
    15781 cgctcaactg gccataatcc ttgtaactga ttaataaaga ttacactgtc tgcgttattt
    15841 cctaactgag gaacgaatcc gggataactt aaccagcgtg gtgcatcggg attgtcccca
    15901 gtcatacggc ggaacgtcgc catcgtgcgc cgctgcatgg tgggctcctg aacaacgata
    15961 gccgtatgaa ctcgttctac ggcctgattc aatagcgcga tgctaaagcg tgcgttttca
    16021 ccgcagtttg ttgactggtc ttcaatccag attttttcat gcggaatgtg ccagaactga
    16081 tgagcgatat ccgccaggat ggtcgcttct gctctgccag tggtgcggat agtgttgtag
    16141 tgcggatgct gtgcgatggc gctatacaaa aaagttgtcg agtgaccgat accaccacta
    16201 atcagtaaag gaatttgctg atcgcgagca atcttacatg ccgcgtcgat agtcggcata
    16261 accgcattgc ctgcaaggat cacgcaatcg gcctgatacg gcacctcacc ggagaaatca
    16321 tcctgcgcca gccactgtcc gataacattt atggcatcta tagttgccgg cgaaagcgtc
    16381 ggaaacgggg taatgttcat ggttcctcct tatcgttccc ttaagaatac tgcgtgagat
    16441 aatagtgaga agcggagcac tctgaaagga ggaagtcgta tttccttaac cgccactccg
    16501 caagcctgac atatctttta aggaaatgct gtagataaac attattcatc ttgcagcaga
    16561 gaattgtatt tttctgaatt cctgaatttt tacccttacc gttt
//
LOCUS       DAZFQD010000117        16590 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.117, whole genome shotgun sequence.
ACCESSION   DAZFQD010000117 DAZFQD010000000
VERSION     DAZFQD010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16590
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.117"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(19..171)
                     /locus_tag="ACHKTW_003823"
     CDS             complement(19..171)
                     /locus_tag="ACHKTW_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO6464759.1"
                     /translation="MLTKYALVAIIVLCFTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            complement(436..1170)
                     /gene="cysH"
                     /locus_tag="ACHKTW_003824"
     CDS             complement(436..1170)
                     /gene="cysH"
                     /locus_tag="ACHKTW_003824"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HFO6464760.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIHPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMSEEETRFFGLKRECGLHEG"
     gene            complement(1244..2956)
                     /gene="cysI"
                     /locus_tag="ACHKTW_003825"
     CDS             complement(1244..2956)
                     /gene="cysI"
                     /locus_tag="ACHKTW_003825"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009337 - sulfite reductase complex
                     (NADPH) [Evidence IEA];
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HFO6464761.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDHEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMAKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            complement(2956..4755)
                     /gene="cysJ"
                     /locus_tag="ACHKTW_003826"
     CDS             complement(2956..4755)
                     /gene="cysJ"
                     /locus_tag="ACHKTW_003826"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0019344 - cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HFO6464762.1"
                     /translation="MTTQVPPSALLPLNPEQLVRLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPAALAATPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDADVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLKGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTPIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     PETPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKD
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            5074..5436
                     /gene="queD"
                     /locus_tag="ACHKTW_003827"
     CDS             5074..5436
                     /gene="queD"
                     /locus_tag="ACHKTW_003827"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HFO6464763.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            5514..6785
                     /gene="ygcN"
                     /locus_tag="ACHKTW_003828"
     CDS             5514..6785
                     /gene="ygcN"
                     /locus_tag="ACHKTW_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFO6464764.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARAGLSVLLLERAEIPGSKNL
                     SGGRLYTHALAELLPQFHLTAPLERCITHESLSLLTPDGATTFSSLQPGGESWSVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHELVTRPAGEAMALGIKEVLSLETSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLTRFKAHPAVRPLIKNTESLEYGAHLVP
                     EGGLHSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDMALTGAQAAAQTLISACQHRE
                     PQNLFPLYHHNVERSLLWDVLQRYQHVPALLQRPGWYRTWPALMQDISRDLWDQGDKP
                     VPPLRQLFWHHLRRHGLWHLAGDVIRSLRCL"
     gene            6776..7036
                     /gene="ygcO"
                     /locus_tag="ACHKTW_003829"
     CDS             6776..7036
                     /gene="ygcO"
                     /locus_tag="ACHKTW_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HFO6464765.1"
                     /translation="MSVARNLWRVADAPHIVPADSVERQTAERLISACPAGLFSLTPE
                     GDLRIDYRSCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            7053..7628
                     /gene="ygcP"
                     /locus_tag="ACHKTW_003830"
     CDS             7053..7628
                     /gene="ygcP"
                     /locus_tag="ACHKTW_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HFO6464766.1"
                     /translation="MPLLHLLRQNPVIAAVKDNASLQLAIDSECQFISVLYGNICTIS
                     NIVKKIKNAGKYAFIHVDLLEGASNKEVVIQFLKLVTEADGIISTKASMLKAARAEGF
                     FCIHRLFIVDSISFHNIDKQVAQSNPDCIEILPGCMPKVLGWVTEKIRQPLIAGGLVC
                     DEEDARNAINAGVVALSTTNTGVWTLAKKLL"
     gene            complement(7776..8636)
                     /gene="ygcQ"
                     /locus_tag="ACHKTW_003831"
     CDS             complement(7776..8636)
                     /gene="ygcQ"
                     /locus_tag="ACHKTW_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HFO6464767.1"
                     /translation="MNIAIVTINQENAAIASWLAAQDFSGCTLAHWQIEPQPVVAEQV
                     LDALVEQWQRTPADVVLFPPGTFGDELSTRLAWRLHGASICQVTSLDIPTVSVRKSHW
                     GNALTATLQTEKRPLCLSLARQAGAAKNATLPSGMQQLNIVPGALPDWLVSTEDLKNV
                     TRDPLAEARRVLVVGQGGEADNQEIAMLAEKLGAEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFVVAINHDASAAVFSQADVGVVDDWKVVLEA
                     LVTNIHADCQ"
     gene            complement(8633..9412)
                     /gene="ygcR"
                     /locus_tag="ACHKTW_003832"
     CDS             complement(8633..9412)
                     /gene="ygcR"
                     /locus_tag="ACHKTW_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HFO6464768.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGKSGPDISLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPE
                     FVARHIAEWQHQNPLDLIITGCQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDALFI
                     TLEQRTEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAEMPA
                     MQCLQLARAEQRRGATLIDGQTVAEKAQKLWRDYLRQRMQP"
     gene            complement(9390..10799)
                     /gene="ygcS"
                     /locus_tag="ACHKTW_003833"
     CDS             complement(9390..10799)
                     /gene="ygcS"
                     /locus_tag="ACHKTW_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO6464769.1"
                     /translation="MTGRCLFGFSGEKPFLLPDNEGVKMNTSPVRMDDLPLNRFHCRI
                     AALTFGAHLTDGYVLGVIGYAIIQLTPAMQLTPFMAGMIGGSALLGLFLGSLVLGWIS
                     DHIGRQKIFTFSFLLITLASFLQFFATTPEHLIGLRILIGIGLGGDYSVGHTLLAEFS
                     PRRHRGILLGAFSVVWTVGYVLASIAGHHFISENPEAWRWLLASAALPALLITLLRWG
                     TPESPRWLLRQGRFAEAHAIVHRYFGPHVLLGDEVVTATHKHIKTLFSSRYWRRTAFN
                     SVFFVCLVIPWFVIYTWLPTIAQTIGLEDALTASLMLNALLIVGALLGLVLTHLLAHR
                     KFLLGSFLLLAATLVVMACLPSGSSLTLLLFVLFSTTISAVSNLVGILPAESFPTDIR
                     SLGVGFATAMSRLGAAVSTGLLPWVLAQWGMQVTLLLLATVLLVGFVVTWLWAPETKA
                     LPLVAAGNVGGANEHSVSV"
     gene            complement(10821..12275)
                     /gene="ygcU"
                     /locus_tag="ACHKTW_003834"
     CDS             complement(10821..12275)
                     /gene="ygcU"
                     /locus_tag="ACHKTW_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HFO6464770.1"
                     /translation="MSLSRAAIVDQLKEIVGADRVITDETVLKKNSIDRFRKFPDIHG
                     IYTLPIPAAVVKLGSTEQVSRVLNFMNAHKINGVPRTGASATEGGLETVVENSVVLDG
                     SAMNQIINIDIENMQATAQCGVPLEVLENALREKGYTTGHSPQSKPLAQMGGLVATRS
                     IGQFSTLYGAIEDMVVGLEAVLADGTVTRIKNVPRRAAGPDIRHIIIGNEGALCYITE
                     VTVKIFKFTPENNLFYGYILEDMKTGFNILREVMVEGYRPSIARLYDAEDGTQHFTHF
                     ADGKCVLIFMAEGNPRIAKATGEGIAEIVARYPQCQRVDSKLIETWFNNLNWGPDKVA
                     AERVQILKTGNMGFTTEVSGCWSCIHEIYESVINRIRTEFPHADDITMLGGHSSHSYQ
                     NGTNMYFVYDYNVVDCKPEEEIDKYHNPLNKIICEETIRLGGSMVHHHGIGKHRVHWS
                     KLEHGSAWALLEGLKKQFDPNGIMNTGTIYPIEK"
     gene            complement(12345..13130)
                     /gene="ygcW"
                     /locus_tag="ACHKTW_003835"
     CDS             complement(12345..13130)
                     /gene="ygcW"
                     /locus_tag="ACHKTW_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO6464771.1"
                     /translation="MSIESLNAFSMDFFSLKGKTAIVTGGNSGLGQAFAMALAKAGAN
                     IFIPSFVKDNGETKEMIEKQGVEVDFMQVDITAEGAPQKIIAACCERFGTVDILVNNA
                     GICKLNKVLDFGRADWDPMIDVNLTAAFELSYEAAKIMIPQKSGKIINICSLFSYLGG
                     QWSPAYSATKHALAGFTKAYCDELGQYNIQVNGIAPGYYATDITLATRSNPETNQRVL
                     DHIPANRWGDTQDLMGAAVFLASPASNYVNGHLLVVDGGYLVR"
     gene            13449..14726
                     /gene="yqcE"
                     /locus_tag="ACHKTW_003836"
     CDS             13449..14726
                     /gene="yqcE"
                     /locus_tag="ACHKTW_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter"
                     /protein_id="HFO6464772.1"
                     /translation="MQHNSYRRWITLAIISFSGGVSFDLAYLRYIYQIPMAKFMGFSN
                     TEIGLIMSTFGIAAIILYAPSGVIADKFSHRKMITSAMIITGLLGLLMATYPPLWVML
                     CIQVAFAITTILMLWSVSIKAASLLGDHSEQGKIMGWMEGLRGVGVMSLAVFTMWVFS
                     RFAPDDSASLKTVIIIYSVVYILLGILCWFFVSDNNNLRSANNEEKQSFQLSDILAVL
                     RISTTWYCSMVIFGVFTIYAILSYSTNYLTEMYGMSLVAASYMGIVINKIFRALCGPL
                     GGIITTYSKVKSPTRVIQILSIIGLLALTALLVTNSNPQSVAMGIGLILLLGFTCYAS
                     RGLYWACPGEARTPSYIMGTTVGICSVIGFLPDVFVYPIIGHWQDTLPAAEAYRNMWL
                     MGMAALAMVIVFTFLLFQKIRTADSAPAMASSK"
     gene            14753..16231
                     /gene="ygcE"
                     /locus_tag="ACHKTW_003837"
     CDS             14753..16231
                     /gene="ygcE"
                     /locus_tag="ACHKTW_003837"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HFO6464773.1"
                     /translation="MSKKYIIGIDGGSQSTKVVMYDLEGNVVCEGKGLLQPMHTPYAD
                     TAEHPDDDLWASLCFAGHDLMSQFAGNKEDIVGIGLGSIRCCRALLKADGTPAAPLIS
                     WQDARVTRPYEHTNPDVAYVTSFSGYLTHRLTGEFKDNIANYFGQWPVDYKSWAWSED
                     AAVMDKFNIPRHMLFDVQMPGTVLGLITPQAALATHFPAGLPVVCTTSDKPVEALGAG
                     LLDDETAVISLGTYITLMMNGKALPKDPVAYWPIMSSIPQTLLYEGYGIRKGMWTVSW
                     LRDMLGESLIQDARAQDLSPEDLLNKKASSVPPGCNGLMTVLDWLTNPWEPYKRGIMI
                     GFDSSMDYAWIYRSILESVALTLKNNYDNMCNEMNHFAKHVIITGGGSNSDLFMQIFA
                     DVFNLPARRNAINGCASLGAAINTAVGLGLYPDYATAVDKMVRVKDIFIPIESNAKRY
                     DAMNKGIFKDLTKHTDVILKKSYEVMHGELGNADSIQSWSNA"
BASE COUNT         3953 a         4360 c         4175 g         4102 t
ORIGIN      
        1 tttacccccg cgtggtggct acttcttcga ttcgtaagcg agaactgcct taaactccat
       61 accacgttct ctgatactca actcacacaa cgagtcgcct accatcagcg tgaatcccag
      121 tactgtaaaa cacagcacta tgattgccac aagggcatat tttgtcagca tgtcttgcct
      181 ccctgcaaag aagagactaa cattcgaatt gtttgggttc gaacgtaagc ctcaggttga
      241 tagaaatatc gcctggggct tttgtccatc tggaacctcg cgaatgctta acgccagaca
      301 gcctcaagca cccgacgcca ttctatacct gataattctt ccagcggcgt tcacgtttaa
      361 aatcaggaat actgctcgca aaataaaaac gcccccatca tttctgacag aggcgtttaa
      421 tttatccggc aatatttacc cttcgtgcaa tccacattcc ctttttaagc caaagaaacg
      481 tgtttcttct tccgacatgc caggttccca tttacgggtt gtatgggtat caccgaccga
      541 taaatatcct tcatcccata atgggtgata tttcaggcca tgtttttgca ggtactgata
      601 aatagttcgg ttatcccagt cgataatcgg cagcacttta aatacgccac gctgaattgc
      661 cagcaccggt aaattggcgc gactgccgga ttgttcacgg cgcaggccag caaaccaggt
      721 ttgcgcgtta agttctttca gagcccggtt catcggttcg actttgttga tgtcattgta
      781 cttttcaatg ccttcaacgc cctgttccca cagtttgccg tagcgtgctt cctgccaggc
      841 ggcgctttcg gtagcgcggt acactttcag gttgagcttg agtttgtccg ttaactcgtc
      901 aataaagcgg taggtttccg ggaacagata gccggtgtcg gtgaggatca ccggaatatc
      961 cgggtgaatc tgattcacca gatgcaggct caccgccgcc tgaatgccaa agctggaaga
     1021 aagtacatat tcaccgggca gattatccag cgcccaggca acgcggcctt cagcgtccag
     1081 tttttccagt tcggcgttag tttctgccag cgccagaatg cgatctacct tcggcagttc
     1141 gttcagggcg tttagatcga gtttggacat agattcctca ctgtttgcct tgcctgatgc
     1201 gacgctcacg cgtcttatca ggcctacaag accgggctga tggttaatcc cacaaatcgc
     1261 gcgccggatc gagcaccggg cgaatgatgc ccgcacgcac cgtaaagtcg ccgaagcctt
     1321 cacccgcttc gcgctctttc gcccagcgcc ctatcagttc atcaagcgac gccaggattt
     1381 ccggctcggt gatgttttct ttatacatcc gtgggatacg tgtcccaatg cggttgccgc
     1441 caagatgcag gttgtagcga cccggcgctt tacccactag gcccacttcc gccagcatcg
     1501 cgcgaccaca accgttcggg cagcctgtta cacgcatcac gatatgctca tcgctgacac
     1561 catgtttcgc cattaaatta tcgatgttgt cgataaaaga cggcaggaaa cgctctgctt
     1621 ccgccatcgc cagcgggcaa gtcgggaacg agacgcaggc catcgagttt tcacgctgcg
     1681 gcgtgacggc attcattaac ccgctctctt tggcgatctt ctcgatcttc gctttctcgc
     1741 tttccggtac accggcgatg atcagattct ggttcgccgt aatgcggaaa tcgcctttgt
     1801 ggatcttcgc gatctccagc aggccggttt tcagcggacg ccccggataa tcaaggatgc
     1861 gaccattttc gataaacagc gtcaggtgcc agttatcatc aatgccctta acccagccaa
     1921 tacgatcgcc tcgtccggtg aactcatatg gacggatcgg ttcaaatttg atccccgcgc
     1981 gacgctccac ttccgcttta aacgtctcaa cccccacgcg ctccagcgtg tatttggttt
     2041 tggcattttt acgatcggtt cggttacccc agtcacgctg agttgtcacg acggcttcgg
     2101 ccaccgccag cgtatgctcc agcggcagat agccaaactc gctcgccgtg cgggcgtagg
     2161 ttttcttgtt gccgtgttcg atagaaagcc caccgcccac cagcaggttg aagcctacca
     2221 gcttgccgtt ttcggcgatt gccacgaagt tcatgtcgtt ggcatgcaga tcgatatcgt
     2281 tctgcggcgg gatcactacc gtggttttga acttacgcgg caggtaggtc tgaccaagga
     2341 tcggttcttc atcagtagtg gcgacttttt cgtggtcgag ccagatctcc gcatacgcgc
     2401 gggtacgagg caacagatgc tcagaaatct tcttcgccca ctcgtacgct tccgcgtgca
     2461 gctgcgactc gtaagggttc gaggtgcaga gtacgttacg gttcatgtcg ttagctgtcg
     2521 ccagcgcatc aagaccgacc gagtgcagca tctggtgcac cggtttgacg ttctttttca
     2581 gaatgccgtg gaactgaaac gtctggcggt tggtcaggcg aatgctgcca tagatggtgt
     2641 tttcaccggc aaatttgtcg atcgcctgcc actgtttagt ggtaatcacc ccacccggca
     2701 gacgacagcg aagcagcatc gcgtggcgcg gctccagctt ctgttcagca cgttcggcgc
     2761 ggatgtcgcg gtcatcctgc tgatacatgc cgtggaagcg gatcagcagg aagttgtcgc
     2821 ccttaaagcc gccggtcaga ccgtcgttta aatcttccgc aatggtgccg cgcaggtagt
     2881 tgctttcaag cttcatgcgc tcggcgtctg tcagttttcc ttcgaccact aaaggccctg
     2941 gatgtttttc gctcattagt agacatctcg ctgataacgg cgctctacgc gcagctcact
     3001 taaaaattca tccgccgctt cggtgtccat gccaccaaat tcggcaatca cttccagaag
     3061 tgcctgctca acgtctttcg ccatgcgatt agcgtcgccg cagacataaa tgtgggcacc
     3121 atcattgatc cagcgccaca gctccgcgcc ctgttcgcgc agtttgtctt gtacgtaaac
     3181 tttttctttt tggtcgcgcg accaggcaag atcgatacgt gtcagcacgc catctttgac
     3241 gtagcgctgc cactccacct gatacaggaa gtcttccgta aagtgcggat taccaaagaa
     3301 cagccagttt ttacctggcg cttcgtcggc ggcgcgttgc tgcataaagg cgcggaacgg
     3361 cgcaataccg gtgcctgggc caatcataat caccggggtt tctggattgg ctggtaggcg
     3421 gaagttatcg ttatgttcga taaatacgcg gacttcgccc tcttcttcca cgcggtcagc
     3481 gaggaagctg gaggcaccac cggcacgggc gcggccttcc acgtcgtaac gcaccacacc
     3541 aacggtgacg tgtacttcgt tctcgacttc cgcctgcgag gaggcaatgg aatagagacg
     3601 cggcgtcagc gggcgcagca gattaattag cgcttcggca tcaagctgtg ccggggagaa
     3661 acgcaccatg tcgacaatcg gcgtcgtcgc ggcgtaatgc tgtaacttcg ctttatcgcc
     3721 caccagcggc agcagtgttt cgctgcgggt aagcgtggcg taattctcaa caatgttggc
     3781 ggtgttgacg gtcagttcga agtgccactg tagcgcttcg ttcagaggca acgttttgcc
     3841 ctcgacggtg acaggttcat cgcctttcag ccacagcagt tcgacaagtt ctttcaccag
     3901 tgccggatcg ttctgatacc agacgcccag cgcgtcaccc ggctggtaac gcaggcccga
     3961 gtcacctaag tcaatttcga tatggcgaac gtctttttca gagttacgcc cggtaatttt
     4021 ctggttaaca gagaggctag ccaccagcgg cgcgtctttg ctgtacgggc tggtgtggat
     4081 ttcatttacc gcgccagtag cgacggattg cgaaggtgcc gcgacaggcg cacgcgattt
     4141 aagcgcatca accacgcggg cgcgccactc gctggcagca gcctggtatt caacatcggc
     4201 atcgacacgg tcgagcaggc gttcaccacc cagttccgcc agcttgctgt caaaatcttt
     4261 cccggactgg cagaaaaatt cataagagct atcgccgagg ctaaacacgg caaatgcggt
     4321 gttttccagc ttcggcgctt ttttggagaa caggaactta tgcagcgcga cggcttcttc
     4381 cggcggttcc ccttctcctt gcgttgacgt caccacgata agcagttttt cgctggcgat
     4441 ttgtttgaat ttatagtcgc ccgcgttcac cagcttaacg ttcagttttg ccgctaataa
     4501 atcatcacgt aatgcttcag caacacggcg cgcattgccg gtttgcgagg cggagataat
     4561 agttataccc ggcatttctg cggcgggagc tggcgtcgct gcaagcgcag caggctgctg
     4621 attgagcacg ccccagaaat agccagaaac ccaggcaagc tgggtgggag ttaaatcggt
     4681 cgtggccgcc tgaaggcgta ccagttgctc cgggttcaac ggaagcaacg cggaaggtgg
     4741 gacctgtgtc gtcatgcgtc gttatgttcc agtaagcaaa gctgtttctg cgccctgtca
     4801 gcgcccataa aacagaagag aaggtaaggt taacggggtg aacggtgtgg attaaagacg
     4861 ggatagcgat aactaataac caaatcgact aacctgtttt agcaatagtc tttaacaaca
     4921 aaatagatta accaacctaa tgaaaaacaa atgaatttag ccaatcatta agataaatca
     4981 gcgattttgc gcaacaaagt cgttttagat aatgcgaaaa aacagccttt ccggtactct
     5041 acggcggttt tatcgtcctg tagagaaatt atgatgtcca ccacgttatt taaagatttc
     5101 accttcgaag ccgctcaccg cttaccacac gtcccggaag ggcataaatg tggtcgcctg
     5161 cacgggcatt cctttatggt gcgactggaa attaccgggg aagtcgatcc gcatacgggc
     5221 tggattatcg atttcgctga actgaaagca gcgtttaagc caacttatga gcgcctcgat
     5281 caccattatc tcaatgatat tccaggcctg gaaaacccga ccagcgaagt tttagcaaaa
     5341 tggatttggg atcaggttaa acccgttgtg ccgctgttaa gtgcggtgat ggtaaaagaa
     5401 acctgcaccg ctggttgtat ctatcgcggc gaatgataag agtgtgtcgg cggtcaattt
     5461 cccttaagta acgctatgtt agggtgttgt gttctggata tctggggcat gacatggaag
     5521 acgactgcga cattattatt attggtgccg gtattgcagg caccgcttgc gcgttacgct
     5581 gcgcgcgagc gggtttatcc gttttgttac tggaacgcgc tgaaatcccc ggcagcaaaa
     5641 atctttccgg cgggcggtta tatacccatg cactcgcgga actcctccct cagtttcatc
     5701 tgaccgcgcc tcttgaacga tgcatcactc acgaaagcct ttccctgtta acgccggatg
     5761 gcgcaacgac gttttccagc ttacagcccg gcggtgaatc ctggagtgta ttacgtgcac
     5821 ggttcgatcc gtggctggtt gccgaagccg aaaaagaagg tgtcgaatgc atccctggtg
     5881 cgacggtgga tgcactgtat gaagaaaacg gcagggtgtg tggtgtcatt tgtggtgacg
     5941 atattctccg cgcccgttat gtggtgctgg cagaaggtgc caacagcgtc ctggctgagc
     6001 gtcacgagtt agtgactcgt cctgctggcg aagcgatggc gttggggatc aaagaagtgc
     6061 tgtcgctgga aacatccgct attgaagaac gttttcatct ggagaataac gaaggcgcag
     6121 cgttgctgtt cagcggcggg atctgtgatg acttacccgg cggcgcattt ctttatacta
     6181 atcaacaaac gctctcgtta gggattgttt gcccgctctc ttcccttacg caaagccgtg
     6241 ttccggcaag cgagctgctg actcgcttta aagcgcatcc ggcagtgcgc ccgcttatca
     6301 aaaacacgga atcactggag tatggtgcgc atctggtgcc agaaggtggc ttgcacagta
     6361 tgccggtgca atacgccggt aacggctggc tgctggtggg cgatgcgttg cgcagttgcg
     6421 tcaataccgg aatttccgtg cgcggcatgg atatggcgct gactggcgcg caggcggcgg
     6481 cacaaacgct gataagcgcc tgccagcacc gcgagccgca aaatctgttt ccgctttatc
     6541 atcacaacgt agagcgcagc ctgctgtggg atgttctaca gcgttatcag catgttccgg
     6601 cgcttttgca acgcccggga tggtaccgta cgtggcctgc gttaatgcag gatatttccc
     6661 gcgatttatg ggatcagggt gataaacctg ttccaccgct gcgccagtta ttctggcatc
     6721 atttacgtcg tcacggcctg tggcatctgg cgggcgatgt tatcaggagt ctgcgatgtc
     6781 tgtagcccgt aatctctggc gcgttgctga tgcgccgcac attgttccgg ctgactccgt
     6841 tgagcgccag acggcagaac ggttgattag cgcctgtccg gcaggtcttt tttcgctcac
     6901 accggaaggt gacttacgta ttgactatcg cagttgcctg gagtgtggca cctgccgttt
     6961 gctgtgcgac gaatcaacac tacaacagtg gcgctatccg ccttccggat tcggcatcac
     7021 ctaccgcttt ggataaataa taaggataat ttatgcccct cttacacctg ctccgccaga
     7081 atccggtgat tgctgccgtt aaagataatg ccagcctgca actggcaatc gattctgaat
     7141 gccaatttat ttccgtgttg tacggcaata tctgcaccat cagtaatatc gttaaaaaga
     7201 ttaagaacgc cgggaaatat gcttttattc atgttgattt gctggaaggc gcgtcaaata
     7261 aagaagtcgt tattcagttt ttgaaactgg tgaccgaggc ggacggcatt atcagtacca
     7321 aagcctcaat gttgaaagcg gcaagagcgg aaggtttttt ctgtattcat cgcctgttta
     7381 ttgttgattc aatttcgttt cacaacattg ataagcaagt tgcgcaatcg aatccggatt
     7441 gtattgagat cctgccaggc tgtatgccca aagtgctggg ctgggtgaca gagaaaatcc
     7501 gccaaccgct gattgccggt gggctggtgt gcgatgaaga agatgcgcgt aatgcgatta
     7561 acgcgggtgt cgtggcgctt tccaccacga ataccggggt ctggacgtta gcgaaaaaat
     7621 tactttgacg ggataatcgt aaccaattga atttggtttg atttgtaggc cgcacgccac
     7681 atccgacatt cagcgcctga tgcgacgctt gacgcgtctt atcaggccta caagtcccca
     7741 ggccagaacc gtaggcagga tcatccggca acacgttatt ggcaatctgc gtgaatgttt
     7801 gtcaccagtg cctccagcac cactttccag tcatcaacca cgccaacatc tgcctgtgaa
     7861 aataccgcag cgctggcgtc atgattaatc gccaccacaa acttgctatt gcgtacgccc
     7921 gccattaacg ctgctgcgcc agaagcgcca acaacaatac acacctcagg tgccagcaga
     7981 tgtccggaaa taccaatcac tttttcagca tcaacgccac cgttcatcac tcgcgcccga
     8041 ctgtagccca cttcggctcc cagcttttct gccagcatag cgatttcctg gttatctgcc
     8101 tctccgccct gccccaccac cagcacccgt ctggcctcag ctaatgggtc gcgagtaaca
     8161 tttttcagat cctctgtgct aaccagccag tccggaagcg cacccgggac aatgttcagt
     8221 tgttgcattc cagagggcaa cgtggcgttt ttcgctgctc ccgcctgacg cgccagggaa
     8281 aggcatagcg ggcgtttttc tgtttgcagc gtcgccgtta gcgcatttcc ccagtgggat
     8341 ttgcgcacgc ttacggttgg aatatctaac gaagtcacct ggcagatgct tgcaccatgt
     8401 aaacgccagg ccagccgggt cgacagttca tcgccaaacg ttcccggcgg aaagagaaca
     8461 acgtccgcag gcgttcgctg ccactgttcc accaacgcat ccagcacctg ttccgccact
     8521 acaggctgag gttcaatctg ccagtgcgcc agggtacaac cgctgaaatc ctgcgccgcc
     8581 agccagcttg ctattgcggc attctcctga tttattgtca caattgcgat attcatggct
     8641 gcatcctttg gcgtaaatag tcccgccata gcttttgagc tttttctgct accgtttgcc
     8701 catcaatcag cgtcgctccg cgtcgttgct ctgctcttgc cagttgcaga cactgcatcg
     8761 ctggcatttc ggcagcaaca gttttacgga taatttctgc tttccccgca gccattcgct
     8821 ggcgcattcc cggtactggc aaggccactt caccgcactg acgcacggca atcaccgcag
     8881 gcaagcgaac ccggcaacaa cgcaacccat gttcagtacg ctgttcgagg gtgataaaca
     8941 gcgcgtcgag agtgaaacgc tccacctggg tgaagcaggg ccagcccagc atttccgcca
     9001 gcaaaaatgg cgtttgccca ttctgccctt cgctgctttg gcagccggta attatcagat
     9061 ccagcggatt ctgatgctgc cattcggcaa tatggcgagc gacaaattcc ggcgcaaagc
     9121 gcagatctgc cgccgtctcc agcaatacag cttcctcaaa cccaagagcc atgagatagc
     9181 gcagccagtg caatgcccgt tcatccccca tgctcaacgc ggttaaagac atcggcgtac
     9241 cgttttttct ctgcgccaac agcagcgcgg cagcggcctg ttcatcagca ccgagtaaac
     9301 ttcgcagtag cgaaatatcc ggtccgcttt taccctgagc cgccgcctgc cactcttttt
     9361 ccgccagcat tccggcatcc ggttcggctt taaacgctaa cagaatgttc attcgcacct
     9421 cctacatttc ccgccgccac cagcgggagg gctttagttt ctggtgccca tagccaggtc
     9481 acaacaaaac caaccaacaa cactgtcgcc aggagcaata aggtgacttg cattccccac
     9541 tgcgccagca cccacggcag caggccagta cttaccgccg cgccaagtcg actcatggcg
     9601 gtggcaaaac cgacgcccag cgagcgaatg tcagtaggaa aactttccgc aggcaaaatg
     9661 cccaccagat tactgactgc cgaaatggtg gtgctgaaga gaacaaaaag cagcagcgtt
     9721 aatgaactgc cggaaggcaa acaggccatt actaccagcg ttgccgccag cagcaaaaaa
     9781 cttcccagca aaaatttgcg atgtgccagc aggtgcgtca gaactaatcc cagcagcgcg
     9841 cccacaatta acaacgcatt aagcatcagg ctggcagtca gcgcatcttc cagaccaata
     9901 gtctgggcga tagttggcag ccaggtataa atcacaaacc atgggattac gaggcagaca
     9961 aagaagacgc tgttaaacgc cgtgcgccgc cagtaacgcg aagagaacaa ggttttgatg
    10021 tgtttatggg tcgccgttac cacttcatcg cccagtaaaa catggggacc aaaatagcga
    10081 tgcacgatag cgtgagcttc tgcaaaacgc ccctggcgca gtagccagcg tggtgattct
    10141 ggcgttcccc agcgtaataa cgtaatcaac aacgcgggca gagctgccga tgccagtagc
    10201 cagcgccagg cctccgggtt ttcggaaata aagtgatgtc cggcaatact tgccagcaca
    10261 tagcctacgg tccacaccac gctgaatgcg cccagcaaaa taccgcgatg gcggcgcggg
    10321 gaaaattcag ccagcaaggt gtgacctact gaataatcgc ctcccagacc aatgccaatc
    10381 aaaatgcgca gtccaataag atgctctggc gtggtggcaa aaaattgtaa aaacgaagca
    10441 agcgtaatca gcaaaaagct gaaggtgaag attttttgcc gaccaatatg gtcggagatc
    10501 cacccaagaa ccaggctgcc aaggaacaaa ccaaggagcg ccgagccgcc gatcattccc
    10561 gccataaacg gcgtcagttg catggcgggc gtaagctgaa taatggcgta accaatgacg
    10621 ccgagaacat aaccgtcggt caggtgtgcg ccgaaagtga gcgcagcaat gcggcagtga
    10681 aaacggttaa gcggtaaatc atccattcgc accggtgaag tgttcatctt taccccttca
    10741 ttgtccggta ataagaacgg cttctcgcct gagaagccga aaaggcaacg ccccgtcatc
    10801 gcggaacgct gcctgacaca ttatttttca atcggataga tagtacccgt attcataatg
    10861 ccattaggat cgaactgctt tttcagccct tccagcaacg cccacgcgct gccgtgttcc
    10921 agtttgctcc agtgaacgcg atgtttaccg ataccgtggt ggtgcaccat cgaaccgccg
    10981 aggcgaatgg tttcttcaca gatgatcttg ttgagcggat tgtggtactt gtcgatttcc
    11041 tcttccggct tacagtcaac gacgttgtaa tcgtagacga agtacatgtt ggtgccgttc
    11101 tgatagctat gagaggaatg accgcccagc atggtgatgt cgtcggcgtg cgggaactca
    11161 gtacgaatac ggttaataac gctttcgtag atttcgtgga tgcagctcca gcagccggac
    11221 acttcagtgg taaagcccat gttgccggtt ttgaggatct gcacacgttc ggcagccact
    11281 ttatccggtc cccagttcag gttgttaaac caggtttcga tcagcttgct gtccacgcgc
    11341 tggcattgcg ggtagcgggc aacgatttcc gcaatccctt cgcccgtcgc cttcgcaatg
    11401 cgagggttac cttcagccat aaagatcagc acgcattttc cgtctgcaaa atgggtgaag
    11461 tgttgggtgc catcttcagc gtcatacaaa cgagcgatcg acggacgata cccttccacc
    11521 atgacttcac gcaggatgtt gaagccggtt ttcatgtctt ccaggatata gccgtagaag
    11581 aggttgtttt ccggggtgaa tttaaagatt ttcactgtta cttcagtgat atagcaaagt
    11641 gcaccttcgt taccgatgat gatgtggcga atgtccgggc cagccgcgcg gcgtggcacg
    11701 tttttaatgc gtgtgacggt gccatcagcc aatactgctt ccagaccaac gaccatatct
    11761 tcgattgcgc cgtagagtgt ggagaactgc ccgatactgc gggttgctac caggccgccc
    11821 atctgagcca gcggctttga ctgcggagaa tgccccgtgg tgtaaccttt ttcacgcaac
    11881 gcattttcca gcacctccag cggaacgcca cattgcgccg tcgcctgcat attctcaata
    11941 tcaatattaa tgatttgatt catggcggag ccgtcgagca ccaccgagtt ttccacaacg
    12001 gtttccagcc caccttcagt ggcggaagcg ccggtacgcg gcacaccgtt aattttgtgc
    12061 gcattcataa aattcagcac acgggatact tgctctgtgg aaccgagttt tacgaccgct
    12121 gccggaatcg gcaaagtata aatgccatga atatccggaa atttacgaaa acggtcaata
    12181 ctgttcttct ttaatactgt ttcatcggta attacgcgat ctgcaccaac aatttccttt
    12241 agttggtcga caatcgctgc gcgagataaa gacatagtaa ttccttcctg ataataaaaa
    12301 tggaataatc attaataaga gaaaacagaa accaccgtta tggattagcg cactaaataa
    12361 ccaccatcga ccactaataa atgcccattg acataatttg aagccggact tgcgaggaat
    12421 acagctgcgc ccattaaatc ctgagtatcg ccccaacggt ttgccggaat atgatcaaga
    12481 acgcgctgat tggtttctgg attactgcgt gtcgccagcg taatatctgt tgcataatag
    12541 ccaggggcaa taccatttac ctgaatatta tattgaccta gttcatcaca ataagctttg
    12601 gtgaatccgg caagagcatg tttagtggca gaatatgcag gtgaccattg tccacctaag
    12661 taagagaaca atgaacagat attaataatt ttgccgcttt tctgcgggat cataattttt
    12721 gcagcttcat agcttaactc gaatgcggcg gtcaggttca catcaatcat cggatcccag
    12781 tcggcacgac cgaagtccag caccttattc agcttacaaa taccggcatt gtttaccaga
    12841 atatcaactg taccgaaacg ctcacagcaa gcagcgataa tcttctgcgg cgcgccttct
    12901 gcggtgatat ccacctgcat gaagtccacc tcaacaccct gtttttcaat catttccttt
    12961 gtttcgccgt tatctttgac gaaactagga ataaagatat ttgcgccagc tttggccaac
    13021 gccatggcaa atgcctggcc taaaccgcta ttcccaccgg taacaattgc ggttttacct
    13081 ttcagtgaga aaaaatccat tgagaacgca ttgagagatt cgattgacat agttagctcc
    13141 aatttccgta aggcaaaaaa aaagaaaggt aacttccccc gcgagaggga agttaccttt
    13201 ctcattatcg ctggtaactg gagataattt ttagcataac ggcataactg ctaatgtgat
    13261 cgtaatcaca gtgtgatatt cgtattgtca aatagtttta aaaagcagaa gcgtgattac
    13321 tctcacacaa cacctctgct ttccatgtta tttcttattg caattactag agacctgaga
    13381 aaagtaatcc acctgaaagg gcggcttact tttctctttt ttttgtctat tacaaatggg
    13441 aatacgatat gcaacacaac tcatatcgcc gttggataac cctcgcgata attagtttta
    13501 gcggcggcgt tagttttgac ctggcttatt tacgttatat ttatcaaatt cccatggcga
    13561 aatttatggg attcagcaat accgagatag gtttaataat gagtaccttt ggtattgcgg
    13621 ctattattct ttatgccccc agcggcgtta ttgccgataa attttcacac cgcaaaatga
    13681 ttacttccgc gatgatcatt accggattac tgggtctgtt aatggcaacg tatccaccgc
    13741 tgtgggtaat gctctgtatt caggtcgcct ttgcgataac gacgatttta atgctgtggt
    13801 cggtgtcgat taaagccgcc tcgttgctgg gcgatcatag tgagcaaggg aaaattatgg
    13861 gctggatgga agggctgcgc ggcgtcggtg taatgtcgct ggcggtgttt accatgtggg
    13921 tcttttctcg ctttgcaccg gatgacagcg ccagcctgaa aacagtcatt atcatctaca
    13981 gtgtggttta catcttgttg gggattctgt gctggttttt tgttagcgat aacaacaacc
    14041 tgcgcagtgc caataacgaa gaaaaacagt cattccagct tagcgacatc cttgccgttt
    14101 tgcgtatcag caccacctgg tattgcagca tggtgatttt tggtgtcttc accatctacg
    14161 ccattctgag ttactccacc aactatctga ccgaaatgta cggcatgtcg ctggtggcgg
    14221 cgagctacat ggggattgtg atcaacaaaa tcttccgcgc gctgtgcggc ccacttggcg
    14281 gcattatcac cacctacagt aaagtgaaat cccctacccg cgtgatccaa atcctttcca
    14341 tcatcggttt gctggcgttg acagccctgc tcgtcacgaa ctctaacccg caatcggtcg
    14401 cgatggggat tggcctgatt ttactgctgg gattcacctg ttacgcctca cgcgggctgt
    14461 actgggcctg ccccggcgaa gcgagaacac cgtcttacat tatgggcacc acggtaggta
    14521 tttgttcggt gattggattc ctgccggatg tcttcgttta cccgattatc ggccactggc
    14581 aagacaccct gcccgctgca gaagcctacc gcaatatgtg gctgatgggc atggcggcgc
    14641 tcgccatggt gattgtcttt acctttttgc tgttccaaaa aattcgtact gctgatagcg
    14701 cccccgcaat ggctagcagc aagtaagcca ggaaaattac aggagaaaaa tcatgtcgaa
    14761 aaaatacatc atagggattg atggcggaag tcagagcaca aaagtggtga tgtacgatct
    14821 ggaaggtaat gtggtttgcg aaggtaaagg cttattacag ccgatgcaca cgccttatgc
    14881 cgatactgca gaacatcctg acgacgattt atgggcatca ttatgttttg caggtcacga
    14941 tttgatgagc cagtttgccg ggaacaaaga agatattgtt ggcatcggcc tgggctccat
    15001 ccgttgctgc cgtgcgttat tgaaagccga cggcacgcca gctgcgccgt tgattagctg
    15061 gcaggatgca cgcgttacac gcccttacga acatacgaat cctgacgtgg cgtatgtcac
    15121 ctctttttcg ggttatctga cgcatcgctt aaccggcgag tttaaagaca atatcgccaa
    15181 ctattttggt cagtggccgg tggattataa gagctgggca tggagcgaag atgctgcggt
    15241 aatggataag tttaatatcc cccgtcatat gctgtttgat gtgcaaatgc ctggcaccgt
    15301 cctcggactt atcacaccac aagccgcact ggcgacacat ttcccggcag gactgccggt
    15361 tgtttgtacc accagtgata aaccggtaga agctctgggg gccggattac tggatgatga
    15421 aactgcggta atttctcttg gcacttacat cacattgatg atgaacggca aagcactgcc
    15481 gaaagatccg gtggcgtact ggccgattat gtcttctatt ccgcaaacat tgctgtatga
    15541 aggttacggt attcgcaaag gtatgtggac ggtgagctgg ctgcgtgaca tgttaggcga
    15601 gtcgttaatt caggatgcca gggcgcagga tctttcaccg gaagatttac tcaacaagaa
    15661 agcctctagt gtgccacctg gctgtaatgg gctgatgacg gtgctggact ggctgaccaa
    15721 tccgtgggaa ccgtacaaac gcgggattat gatcggcttt gattccagca tggattacgc
    15781 atggatatat cgttcgatat tggaaagcgt ggcgctaacg ctgaagaaca attacgacaa
    15841 tatgtgtaat gaaatgaatc actttgcgaa gcatgtgatc attactggcg gcggttcgaa
    15901 cagcgatctg tttatgcaaa tttttgccga cgtgttcaac cttccggcac gccgtaacgc
    15961 cattaacggt tgtgcaagtc tgggagcagc gattaataca gcggtaggtc tggggctata
    16021 cccggattac gcaacggctg ttgataagat ggttcgcgtg aaagatatct ttataccgat
    16081 tgagagcaat gccaaacgct acgacgcgat gaataaaggc attttcaaag acctaaccaa
    16141 acatactgat gtgatcctga aaaaatcgta tgaagtgatg catggggaat tggggaatgc
    16201 ggattcgatc cagagctggt cgaatgcgtg aataaggtag gccgggcgga aaactgcccg
    16261 gcctgaacat acctgaatgg ttatccccgc tgacgcgggg aacataagtt cgacaactta
    16321 aaaaatgata acaagtccgg tttatccccg ctgatgcagg gaacataata aaaactttaa
    16381 ctagttcggt ttatccctgt ttttacaagg aatattaact acactcgatg tctttagaat
    16441 aaaagacaat acgaatcgca ctttatcaat tttcacttaa agtatgaact ggatatcttt
    16501 attcataaga actattcatc aacagcgtat agaggcggtt atgtaaaacc actcattagc
    16561 ctcaaaacaa agacaaactt ccagatttat
//
LOCUS       DAZFQD010000118        16567 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.118, whole genome shotgun sequence.
ACCESSION   DAZFQD010000118 DAZFQD010000000
VERSION     DAZFQD010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16567
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.118"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            90..708
                     /locus_tag="ACHKTW_003838"
                     /pseudo
     CDS             90..708
                     /locus_tag="ACHKTW_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416774.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            811..1314
                     /gene="yfbM"
                     /locus_tag="ACHKTW_003839"
     CDS             811..1314
                     /gene="yfbM"
                     /locus_tag="ACHKTW_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
                     /protein_id="HFO6464774.1"
                     /translation="MGMIGYFAEIDSEKINQLLESTEKPLMDNIHDTLSGLRRLDIDK
                     RWDFLHFGLTGTSAFDPAKNDPLSRAVLGEHSLEDGIDGFLGLTWNQELAATIDRLES
                     LDRSELRKQFSIKRLNEMEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNH
                     ALTVIVG"
     gene            complement(1381..2838)
                     /gene="nuoN"
                     /locus_tag="ACHKTW_003840"
     CDS             complement(1381..2838)
                     /gene="nuoN"
                     /locus_tag="ACHKTW_003840"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HFO6464775.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGLMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(2845..4374)
                     /gene="nuoM"
                     /locus_tag="ACHKTW_003841"
     CDS             complement(2845..4374)
                     /gene="nuoM"
                     /locus_tag="ACHKTW_003841"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HFO6464776.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMAFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(4538..6379)
                     /gene="nuoL"
                     /locus_tag="ACHKTW_003842"
     CDS             complement(4538..6379)
                     /gene="nuoL"
                     /locus_tag="ACHKTW_003842"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HFO6464777.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWLGKRTLVTSIANSAPGRLLSTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(6376..6678)
                     /gene="nuoK"
                     /locus_tag="ACHKTW_003843"
     CDS             complement(6376..6678)
                     /gene="nuoK"
                     /locus_tag="ACHKTW_003843"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HFO6464778.1"
                     /translation="MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(6675..7229)
                     /gene="nuoJ"
                     /locus_tag="ACHKTW_003844"
     CDS             complement(6675..7229)
                     /gene="nuoJ"
                     /locus_tag="ACHKTW_003844"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HFO6464779.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(7241..7783)
                     /gene="nuoI"
                     /locus_tag="ACHKTW_003845"
     CDS             complement(7241..7783)
                     /gene="nuoI"
                     /locus_tag="ACHKTW_003845"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HFO6464780.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(7798..8775)
                     /gene="nuoH"
                     /locus_tag="ACHKTW_003846"
     CDS             complement(7798..8775)
                     /gene="nuoH"
                     /locus_tag="ACHKTW_003846"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HFO6464781.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDQVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(8772..11498)
                     /gene="nuoG"
                     /locus_tag="ACHKTW_003847"
     CDS             complement(8772..11498)
                     /gene="nuoG"
                     /locus_tag="ACHKTW_003847"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HFO6464782.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQSKIDVIVQALAGAKKPLIISGTNAGSIE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRHASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSAASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     HVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(11551..12888)
                     /gene="nuoF"
                     /locus_tag="ACHKTW_003848"
     CDS             complement(11551..12888)
                     /gene="nuoF"
                     /locus_tag="ACHKTW_003848"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HFO6464783.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(12885..13385)
                     /gene="nuoE"
                     /locus_tag="ACHKTW_003849"
     CDS             complement(12885..13385)
                     /gene="nuoE"
                     /locus_tag="ACHKTW_003849"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HFO6464784.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(13388..15190)
                     /gene="nuoC"
                     /locus_tag="ACHKTW_003850"
     CDS             complement(13388..15190)
                     /gene="nuoC"
                     /locus_tag="ACHKTW_003850"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030964 - NADH dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HFO6464785.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(15284..15946)
                     /gene="nuoB"
                     /locus_tag="ACHKTW_003851"
     CDS             complement(15284..15946)
                     /gene="nuoB"
                     /locus_tag="ACHKTW_003851"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HFO6464786.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(15962..16405)
                     /gene="nuoA"
                     /locus_tag="ACHKTW_003852"
     CDS             complement(15962..16405)
                     /gene="nuoA"
                     /locus_tag="ACHKTW_003852"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HFO6464787.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
BASE COUNT         4096 a         4678 c         4337 g         3456 t
ORIGIN      
        1 ataaaacaaa cgctcataac tcattataaa taatgataaa ataacgcctt ttattgataa
       61 tttacatatg aaaaaaattt ccttttgtta tattatttct gttttcactc cctctgatta
      121 ttttttacca accctgggtc actgcattat cgccaatgcc ccgacacgta agtcctgagc
      181 aattagaaaa aacagttcgt tatcttacac aaactgtgca tccacgtaat gccggcaata
      241 ttgataatct gaataggtcc gtagagtata taaaagaggt ctttgtcagt agcggtgcca
      301 gagttaccgc gcagggcgtc cccgattggc tattacgact ctgcgcctgg aagccagaat
      361 tacccttacc cggcaatgtc ctggctttat cccgatcggg gagactttat tgccgtagtc
      421 ggcagaatgc aggatatcaa cgccgttcgt caggtaaaat cggcattgtt gtcatcccgg
      481 gatttatctg tttattctat gaatgcccca gggtttattc ccggtattga tttctctgat
      541 caccttaata attggcaaca cgatattccc gccgtaatga ttactgacac cgctttttat
      601 cgtaataaac aataccactt gcccggtgat accgcagaca gattgaatta tcagaaaatg
      661 gctcaggtgg tggatggtgt tataaacttg ttatacaaca gtaaataata aaaatttctc
      721 gaggcgggtc cagaaaaagc gacatgcaca ttttattttc cctcgtctgt tttaagatgc
      781 gaaacatgaa attcaatgga ggaaaaataa atgggaatga ttggctattt tgcagaaatt
      841 gactcagaaa agattaacca actgcttgag tctactgaaa aacccttaat ggacaatatt
      901 catgacacgc tttcaggatt acgccgactg gatattgaca aacgctggga ttttttacac
      961 tttgggctga caggcacctc tgcttttgat cccgcgaaga atgatccgct gagtcgtgcc
     1021 gtgcttggtg aacacagcct cgaagatggc atagatggct tcctgggatt gacctggaat
     1081 caggagttag ccgcgacaat cgaccgactg gaatcactgg atcgaagtga gctgcgtaaa
     1141 cagttttcca ttaagcgact caatgaaatg gagatttatc cgggagtgac cttttcagaa
     1201 gagttagagg ggcaactctt tgcctcgatc atgcttgata tggaaaagct gattagcgcg
     1261 tatcgacgta tgctgcgaca ggggaatcat gcgctgacgg ttattgttgg gtgatggtgt
     1321 cagggcacca aaaaaaaccg ccgaacatgt cggcggcttt ctgacttaca aactgatagc
     1381 ttacatcagc ggcattgcta accgcacaat gctaatcagc ggttgtggcc atacacccag
     1441 caccagtacc aacagtgcag aaatcagcac cacgataccg cccgcgctgt actgccagtt
     1501 tgacggtgca tcgcgacccg gttgttccgg tgcgtgcagg tacaggctca ccgccacgcg
     1561 caggtagtag tagaggccga ttgccgaacc gacaaccaca gcacccacca gccaccacaa
     1621 gtgtgcctgg acaccgaccg ccagcacgta gaacttaccg ataaagccca gcgtcatcgg
     1681 gataccggcc agagacagca tcatcaccgt catcaccgcc gcgaggatcg ggcgatgcca
     1741 gaacagaccg cggtaagaga acagggaatc agcatccggg ccacggtacg ggctggacat
     1801 caggctgacc acgccgaacg cgccgaggct gctgaacaga taaccggcca ggtaaactcc
     1861 taccgcttcc atcgacatct cgcccgtttg cagcgcaatc agcgccacca gcagatagcc
     1921 gaggtgagag atagatgagt aaccgagcag acgtttgata ttggtctggc tcagcgccat
     1981 caggttaccg aagatgatag aggcaaaggc gataatcgcc agcactaccc gaatcgcttc
     2041 gctgtcaccc accggtgcgt agaggaacag acgcatcacc acaccgaaga tagcgatttt
     2101 gctcgccgtc gccaggaaag tggaaaccgg cgcaggcgcg ccctggtata cgtctggcgt
     2161 ccacaggtgg aacggcacca gagagagttt gaagccaagg ccaacaatca tcaggccgaa
     2221 acctgccagc aacagcggct cgttgagcat accgtcgccg aggtttttac ccagcgcgac
     2281 aaacgacagg tcgccagact gcgcatacac cagcgccata ccgaacagca ggaaagaaga
     2341 cgctgcggca gaaaggatag tgtatttgat actggcttcc agcgaacgtt tctggcggaa
     2401 agcgtaaccg accaggccaa acagcggcaa agagatcagt tcgataccga ggaacagaga
     2461 cgccagatgg ttggcattcg ccagcaggat cccgcccagc gcggcaatta acaccaacag
     2521 gtagaactca tccttgttgt cgttatagcc ttcaagccac gggtaggcga aagtacaggt
     2581 ggcgaggctc gccaacaata ccagcccggt gtaaagcatg gcgaaaccat caacgcgcat
     2641 gagcggcgta acgtccatag cgcccgcctg gccaacaaac cagagcgaaa ccagcgccgc
     2701 gttaagccca ataaccgaga gcgtagcgtt gaggaaatga ttgcgtcgcc acgcaatgga
     2761 gagcatcaca accaccaccg tcaagccgac gatcagcaac ggtagcagtg cgatcaggtt
     2821 ttgtggagtt attgtcatgg cgatttacgg ccttgtagta gtaacggaat taacaaacca
     2881 ctgctggata ttgccaatcg cggagtgcga ggtatccaga atcggctgcg gatagaagcc
     2941 cagcagtacc agcagcacca ccagcaacag gatcataaac agctcacgca gcgacatccc
     3001 tggcagttcc tggctggcaa tctggctttt cgctttaccg aagtaagcgc gatgcaacat
     3061 cgccagcgaa taaacagatg caaagaccag cccaaaggta gagataacgg taatcaccgg
     3121 gacaacctgg aagctgccga acagaatcat aaattcgccg acgaagttac cggtgccagg
     3181 catcccaagc gttgccaccg caaagaacag cgacagtgct ggcagccatt tcatcttgct
     3241 ccacagaccg cccatcatgc gcatgtcgcg ggtatggata cgttcataaa gctgaccaca
     3301 aagaataaac agacccgccg ccgacaaacc gtgcgcaatc atctggatta ccgcgccctg
     3361 gtaggccaac tggctgccgg tgtagatagc aatcagcacg aagcccatgt gggaaaccga
     3421 ggtgtaggcg atcagacgtt tgatatcggt ctgggcgaag gccatccacg caccgtagaa
     3481 gatgccgata acacccagcc acatagcaat tggcgcgaac tctgccgacg cgttcgggaa
     3541 cagcggcagg gagaaacgca gcaaaccgta agcggcagtt ttcagcaaga tccccgcgag
     3601 gtcaacggaa ccggcggtcg gagcctggga gtgcgcatcc ggcagccagc catgcagcgg
     3661 aaccaccggc attttgactg cgaaggcgat gaagaagccc agcattaaca ggtattccac
     3721 accactggac attggcgtat tcagcagctc ttcatagttg aaggtccaga cgccggtcgc
     3781 attgtagtga acaaaaacca gcgccaggat ggcaatcaac atcaccaggc cactcgcctg
     3841 ggtgtaaatg aagaacttgg ttgccgccgt gatacgcgtt ttaccgtcag aggctttatg
     3901 cccccacagt gcgatcagga agtacatcgg caccagcatc atttcccaga agaagaagaa
     3961 caggaacatg tcgatggcaa ggaacacgcc gataacgccg cccaggatcc acatcaggtt
     4021 gaggtggaag aagccctgat atttttcgat ctctttccac gaacatagta ccgccagcac
     4081 accgagcaga ccggtcagca cgaccatcag cagcgacaga ccgtcaatgg cgagatgaat
     4141 agagatacca aaacgcggga tccacggcat gtcgaattca gactgccact gcggaattcc
     4201 ggcggattgc gtcagtgaat aaccgccctg caaccacagt tgcagcgaca gcgccagcgt
     4261 caatcccatg gtgatcagcg cgatccagcg cggcaccttg acgccaaagc gttcggtctg
     4321 ccagcacagg aagccgccaa taaagggaat taatattagc cagggtagta acatggcgat
     4381 ctttattcct tgtaaaagtc ccgtcaggac cggattttca acgaattctc acgacagaaa
     4441 attctcttcg gactgggggt agtgtcggat gcgccgcgaa ggcagcttat ccgacctacg
     4501 ttcttatatg cccaggggca atcccacaat ccttaactca acgcagtacc atcaacagtg
     4561 ccagcaccac gaccgcaccg atgctcatgg atgccacata ccagcgcaga taaccgttct
     4621 cgcttaacag cagaccttta cctgcaaagc gggaaagaac cgccgggatg ttcatcattg
     4681 agttcagcgg atcgcgtttc agcaaccagg caatacccag gaacggcttg acgaacactt
     4741 tgtcgtacag ccagtcaaag ccccaggcgt tgtaccacca ggtactcagc aggcggcccg
     4801 gcgcactgtt ggcgatggag gtcaccagag tacgtttacc cagccacagc caggctgcca
     4861 gcagaatgcc gaccaccgcg accacgccag aggtaatttc cagggtcaac atgctgccgt
     4921 gcgccagttc cgtcgtttgc ggaagcacgc cctgcagcgg cggtacaatc agtgcgccaa
     4981 cgaaggtgga aaggatcagc agcacaatca gcggcaggct gtgagttacc cctttcacgg
     5041 cgtgagcgtg aatttgttct tttccgtgga agacgatgaa aatcatacgg aaggtgtaga
     5101 gcgaggtcat aaacgcaccg accagacctg ccaccatcag attgatatga ccattcgcca
     5161 tcgcacccgc gaggatctca tccttactga agaagcccgc agtgaccagc ggtagtgccg
     5221 acagtgctgc gccgcccacc aggaagcaga gataaaccag cggaatagat ttacgcagac
     5281 cgcccatctt gaagatgttc tgttcgtgat ggcaggccag aatgacggaa ccggatgcca
     5341 ggaacagcag cgctttaaag aacgcgtgag tcatcaagtg gaaaatcgcc gcatcccatg
     5401 cctgcacgcc aagcgcgagg aacatgtagc caatctggct catggtagag taagcgagaa
     5461 cacgtttgat gtcggtctgt accagcgcgg caaaaccggc cagcagcagc gtaaccgccc
     5521 cgacaatacc caccagatgc agaacttccg gcgtcatcag gaacaggccg tgggtacggg
     5581 cgatcaggta gacacccgcg gttaccatcg ttgcggcgtg gatcagcgcg gagacaggcg
     5641 tcgggcccgc catcgcgtca gcaagccatg tctgcaacgg caactgcgca gatttaccga
     5701 ccgcaccgcc cagcagcatc agcgtcgccc acatcagcat gttattgccg tcagcaaagt
     5761 gcgctggtgc cagttccacc atttcgcgga agttcagggt gcccagttcg ttgtaaagaa
     5821 tgaacagtgc gaaagcgagg aaaacgtcac ccacacgggt cacgacgaac gctttcattg
     5881 ccgctgcgcc attcttcgga tcggtgtaat agaaaccgat cagcagatag gagcacaggc
     5941 ccacgccttc ccagccgagg tacatcagca gcaggttatc ggcgagaacc agaactacca
     6001 tgctggcgat gaacaggttg gtgtaggcga agaagcgaga gtagccctct tcaccgcgca
     6061 tgtaccagga ggcgtacatg tgaataagga aacccacacc agtgaccacc gagagcatgg
     6121 tcagcgacag gccgtccagc accaggttaa aaccgatgtt aaagtcgcct accgacatcc
     6181 acgtccacag cggctggctg tatgcctgct cgccgttagc gaagaaatca acgccgataa
     6241 aggcggttac cagcgccgcc aggcccacag agccaacgcc gacgatcgcc gaaacgtttt
     6301 cagaccagcg cccacgggag aacgccagca ggacgaagcc aatcaatggc aaaataatgg
     6361 ttaaggcaag catgttcatc cgcgcatctc acttactgaa tcgatgttca ggttctggcg
     6421 acgacggtga agttgcagca gcagcgcaag gccgatactc gcttctgccg ccgcgaggct
     6481 gatggcgaga atgtacatca cctgaccgtc ggtctgaccc cagtagcttc cggcgaccac
     6541 aaaggccagc gcggaggcgt taatcatgat ttccagacca atcagcataa acagcagatt
     6601 gcgacggata accagaccgg ttaagccaag aacgaataag attgccgcga ggatcagtcc
     6661 atgttgtaag gggatcatgc gtgctcctcc gtttttcttt tcgcgctgtc gtctttacga
     6721 ttgctcagca cttcacccgc acgctcttca cgaccgacgt ggaaggccac aaccagaccc
     6781 gcgagcagca gcatagaagc cagttccacc gccagtacgt aaggcccgaa cagcgtaata
     6841 cccactgctt tagcactgat tggcgtaccg tcgataccct gatcattaac accaaggatg
     6901 gcgtaaacaa tcaccaccag catgatggcc gacaaaattg ccggaccaat ccacacctgc
     6961 ggtttcagcc actggcgttc ctgttcgatt tctgaaccgc ccaggttgag catcatcacc
     7021 acgaacacga acagcaccat aatggcaccc gcgtagacga taatttccag cgcaccggcg
     7081 aagtaagcgc ccagtgagaa gaacaccccg gaaatcgcca gcagcgaaat aatcaggtac
     7141 agcagtgcgt gtaccggatt ggtatgggtg atcactcgca aggtcgcaag tatggctatc
     7201 aggccacaga tataaaaagc gaactccatt gcccctctcc ttacggtaac aggctcttga
     7261 cgtcgatagg cttggcttcg ttctctgctt cgcccttatc tttgccgtcg attgccatac
     7321 ctgccatccg gtagaagtta tattccgggt atttgcccgg accggagatc agcagatcct
     7381 ctttctcgta aaccagatcc tggcgcttgt attcccccat ttcgaaatcc ggggttaact
     7441 gaatcgccgt ggtcggacag gcttcttcgc acagaccaca gaaaatgcag cgtgagaagt
     7501 tgatgcggaa aaattccggg taccagcgac cgtctttggt ttctgctttt tgcagcgaga
     7561 tacagccgac cgggcaggct accgcgcaga ggttacaggc tacgcaacgc tcttcgccgt
     7621 ccgggtcgcg ggtcagaacg atacgaccac gataacgggg cggcagatag accggctctt
     7681 ccgggtacat tcgcgtttcg cgtttggcga acgcgtgcag gccgatcatc cagatactac
     7741 gaacctgggt gccgaaacct actaacaatt cttttaaggt catggtctta ttgcccctta
     7801 ttgcgcctgc cagagaatga cagccgccgt taccagcaag ttgatcagcg tcagcggcag
     7861 gcagattttc cagccgaagg acattacctg gtcataacgc ggacgcggta acgacgcacg
     7921 aatcaaaatg aacatcatca taaagaacgc ggttttcagc gcgaaccaga tgaatggcgg
     7981 taacaacggg ccttgccagc caccgaagaa cagcgtcacc atcaatgcag agatggtcac
     8041 aatcccgatg tactcaccca cgaagaacag accgaacttc ataccggaat attcaatgtg
     8101 gtaaccatcc gccagttcct gctcggcttc cggctggtca aacgggtgac ggtgacatac
     8161 cgccacgccc gcgatggcaa aggtaataaa accaaagaat tgcgggataa cgttccacac
     8221 atgcgcctgg ctgttgacga tgtcggtcat gttgaatgaa ccggcctgcg ccaccacgcc
     8281 catcagggaa agcccgagga acacttcgta gctcagggtc tgcgcagaag cacgcatcgc
     8341 acccagcaac gagtatttgt tgttacttga ccagcccgca aacagcaccg cgtaaaccgc
     8401 cagacctgcc atcatcagga agaacaaaat cccgatgttc aggtcggcaa ccacccaacc
     8461 cggactgact ggcacaatcg caaaggccag cagcagcgag gtaaaggcaa tcatcggtgc
     8521 cagggtaaag atgacgcgat ccgagaattt cgggatccag tcttctttaa agaacatttt
     8581 gatcatgtcc gcaaccagct ggagcgaacc gccccagcca acacggttag gtccgtaacg
     8641 gttctggaac agacccagca ggcgacgttc gccaaagctc atgaatgccc cgcaggtgac
     8701 aaccaccagc aggatcacca ccgctttgag gatggtcagc aggatctcaa tcagttccgg
     8761 tgatatccaa ctcattgttg tgcctccttg agatcctcaa gatgcgcgcc agccagcacc
     8821 ggagcaatac cggacatacc catcggcaag cccacctgcc ctgccgtcag cccttcagcg
     8881 atttcaaccg gcagcgtgac cgtgttgcca tcgtaactaa aggagacgtg tgtacctgcg
     8941 ttcacaccta acttcgcggc atccgctggg ttgagtttga tgtacggctg cggcatacgg
     9001 ctctggaaga ccggagcacg ctgtgacaat tcatcgctgc caaacaggtg gtaatacggc
     9061 gcgatacgcc atttcccgtc ctgcggctgg aagcgtgccg gtacgctggt gaagtaatcc
     9121 agaccatttt cgctggtttc aaacagacgc acgcccggat cgccaaagcg cagtttgccg
     9181 cccacttcgt cctggaattt gttccacgcc tgcggggagt tccagcccgg cgcccaggca
     9241 aacggcactt gcgaacggtg cgcagtcggc tggttgttac cttccatcga gaaggtgaac
     9301 atggtgtcaa tatcctgcgg ctgacgcggc tcatgaacgc tgatattggc gcgcatggcg
     9361 gtacgaccgc tgtaacggtg cggttcacgg gccagtttct gcccacgaat acggaatgtc
     9421 gcatccggcg cagcatcttt gatacctgcc agttccggga ttttcgccac aacagcgtca
     9481 atcacatggt cgagctgcgt ccagtccact tcacggctca gcagggtgct gtgcagcgag
     9541 tgtaaccagc gccagctttc cagcatgaca gttttgctgt cgtaataagc aggatcgtaa
     9601 acctggaaga aacgttgggc gcggccttcg ttgttgatca ccgtaccgtc gctttcagca
     9661 aagctggcag cagaaagtac cagatgggcg ttttccataa tcgctgtgcg ttgatgatca
     9721 accaccatca ccagcggcgc tttagccagc gcagcattca cgcgggtagc ggaagcgtga
     9781 cgatgcagat cgttttccag caccaccacc gcgtcggcgc gtccggtttc cagttcggtc
     9841 aacgcttctt caagcgaacc gccacccata atgcccagcc ccatgctgtt gacggaacgg
     9901 gcaatcatgg tgataccaac gtcagcgccg cgacctttca gggctttcgc gacgttagcc
     9961 gcagcctgaa tcacctctat gctaccggcg ttcgtcccgg agataatcaa cggtttcttc
    10021 gcacctgcca gtgcctgcac gatgacgtcg attttgcttt gcagctcagg ttcgataccg
    10081 tcaaccgctg gcgcagagtt atccagcgca tgggcgatgg caaaacctaa acgcgcctga
    10141 tcttcaaccg gtgcgcggta agtccacgcc gcgatatcat ccagacgggt gtcatcaacg
    10201 ttggtaacaa acagcggatg cttcgcacgt tgaccgatgt tgaggattgc cgcaatctgc
    10261 cagtcagcca ctttctgtgc tgccgccatt tcgcgcgctt tacctttcac agcctgacgc
    10321 actgccagcg cgacgcgcgc gccggtctgg gtaacgtctt cgcccagcac cagtaccgca
    10381 tcgtaagatt cgatttcgcg cagagccgga gtataaatgc cgccttcacg cagcactttc
    10441 agcgccagtt gcagacgttc ctgctcaccg tgagcgatac cggtgtagaa gttttcttcg
    10501 cccaccagtt cacgcagcgc aaagttgctt tccacgctgg cacgcggaga accaataccg
    10561 atcactttct tcgactgacg cagaatatct gccgcgccct gcattgcctg ttcggcgttg
    10621 agggtaatga aatcatcgcc acgacgctgt actggttgac gcggacgatc tttcaggttg
    10681 acgtaaccgt aaccgaaacg accacggtcg cagaggaagt agtggtttac cgtaccgttg
    10741 taacggtttt cgatacgacg cagttcgccg taacgttcac cggggctgat gttacagccg
    10801 atggaacatt gctggcagat gctcggcgca aactgcatat cccatttacg gttgtaacgc
    10861 tcggagtgcg ttttgtcggt aaatacgccg gtcgggcaaa tttcgaccag gttaccggaa
    10921 aattcgcttt ccagcgtgcc gtcttccggg cgaccgaagt agacgttgtc gtgcgcaccg
    10981 taaacgccca gatctgtacc gtcagcgtaa tctttgtagt aacgcacaca gcggtagcag
    11041 gcgatgcagc ggttcatttc gtgagagatg aatggcccca aatcctgatt acggtgggta
    11101 cgtttggtga aacggtagcg acggaagctg tgtccggtca tcacagtcat atcctgaaga
    11161 tggcagttac cgccctcttc acataccgga cagtcgtgcg ggtggttggt catcaaccac
    11221 tcgaccacgc tttcacggaa ctgtttcgct tcttcgtcgt caatggaaat aaaggtgcca
    11281 tcggaagccg gtgtcataca ggacatcacc aggcgaccac gcgtgtcttc cgcgttttgg
    11341 tattgcttca ccgcacactg gcggcaagca ccgacgcttc ccagcgccgg atgccagcaa
    11401 aagtaaggaa tatcaaggcc cagagacaga caagcttcca gcaggttgtc cgctccgttg
    11461 acctcgtatt ctttgccgtc tacatgaatt gtagccatta gcatgcttcc agttttctcg
    11521 gtcagggacc gagcgttaat cgaaattcgg ttaccagcgc tctttcagca ggttcggctg
    11581 aatcccatta atcaaatggg tattgctgaa cggctgtttg attcccgcct caaattcttc
    11641 gcggaaatat ttgatggcgc tctgtaacgg ctccactgca ccaggtgcgt gggcacagaa
    11701 agttttaccc gggcctaaga atcgacacag ttgctcaagt gtttcgatat cgcccggctg
    11761 accttcacca cgctccagcg cacgcagaat tttcacgctc cacggcagac cgtcgcggca
    11821 cggcgtacac cagccgcagg actcacgggc gaaaaactct tccaggttac gcaccagcga
    11881 caccatgttg atctcatggt caaccgccat cgccagcgcc gtacccagac ggcttcccgc
    11941 tttaccgata ctttcgaatt ccatcggcag gtcaaggtgc gcttcggtca ggaagtcggt
    12001 gcccgcgccg cctggctgcc aggctttgaa tttcagaccg tcacgcatac caccggcgta
    12061 atcttcgagg atctcgcgcg cggtagtacc aaacggcagt tcccacagtc ccggattttt
    12121 cacccgaccg gagaagccca tcagcttggt gccagcatct ttacttttcg agatgttctg
    12181 ataccactcc acgccgttag cgaggatcgc cggaacgtta cacagggttt cgacgttgtt
    12241 gacacaggtc ggtttgcccc atacgccgga ggttgccggg aaggggggtt tcgagcgtgg
    12301 gttagcacga cgcccttcca gggagttgat taacgctgtt tcttccccgc agatgtagcg
    12361 ccctgccccg gtatggacga acagttcgaa gtcgaaacct gttcccataa tgtttttgcc
    12421 aagcaagccc gcttcagtgg cttcggcaat ggcgcggcgc agatttactg ccgcttcgat
    12481 atattcgcca cgcaggaaga tgtagccacg gtaagctttc agcgcaaacg cggagatgag
    12541 cataccttcc accagcaggt gcggcagttg ctccatcagc aggcggtctt tataggtgcc
    12601 tggctccatt tcatcggcgt tacacagcag gtaacggatg ttcatggatt cgtctttcgg
    12661 catcaggctc catttcaggc cggtagaaaa gcccgcgcca ccgcgccctt tcagaccagc
    12721 gtcttttacc tgattaacga tttcgtccgg agacagcccg gtcagcgcct tacgcgcgcc
    12781 ttcgtaaccg tttttgctgc gatattcgtc cagccacact ggctgtttgt catcgcgcag
    12841 acgccaggtc agcggatgcg tttcgggagt acggataatg tttttcattt ataccgctcc
    12901 agcagttcag ggatcgcttc cggggtcaga tgcgcgtgag tatcctcatc gatcatcatg
    12961 tttggccctt tatcacagtt ccccaggcag caagttggca gcagcgtaaa gcgaccgtca
    13021 aatgtcgttt gccctggttt gatgtttagc tttttctcga gcgccgcctg aataccctgg
    13081 taaccgttga tatgacagac cacgctgtcg caataacgga tcacatggcg gccaaccggc
    13141 tggcggaaga tctggctgta gaacgttgcc acaccttcga cgtcgcttgc cggaataccc
    13201 agcacatcgg cgatcgcgtg gatcgcacca tccggcaccc agccacgctg cttctgaacg
    13261 attttcagcg cttcaatgga cgccgcacgc gggtcttcgt agtggtgcat ctcgtgctca
    13321 atcgcttcac gctctgccgc actcagctca aaagcctcgg tttgtggttg ttgattctcg
    13381 tgcataatta gcggtccaca tctgacataa caaaatcgat actgcccaga taaacaatca
    13441 ggtcagacac caggctgccg cggatcgccg ccggaatttg ctgcaaatgc gcataactcg
    13501 gcgtgcggat acgagtgcga taactcatgg tgctaccgtc gctggtcagg tagtaactgt
    13561 tgatcccctt ggtcgcctca atcatctgga aagattcatt ggcaggcatc accggccccc
    13621 acgacacttg caggaagtgg gtgatcaggg tttcgatatg ttgcagcgtg cgctctttcg
    13681 gcggcggcgt ggtcagcggg tgatccgctt tgaacgggcc ttccggcatg ttgttgaggc
    13741 actgctcaag aatgcgcaga ctctggcgca gctcttccac tttcagcatt acgcgggtgt
    13801 agcagtcaga aacaccacca ccaaccggga tttcaaagtc gaagttttca tagccagaat
    13861 aaggacgcgc cttacgcacg tcgaagtcga tcccggtagc acgcaggccc gcgccagtgg
    13921 tgccccactc cagcgcctct ttcgcgccat aggcggcaac gccctgggaa cgacctttca
    13981 gaatggtatt ttgcagcgcc gctttctcgt aagacgccag acgtttcggc atccagtcga
    14041 ggaactcacg cagcaggcga tcccagccgc gcggcaggtc gtgcgctacg ccgccaatac
    14101 ggaaccacgc cgggtgcata cggaaacccg tgatcgcttc caccagatcg taaattttct
    14161 gacgatcggt aaaggcgaag aacacgggcg tcattgcgcc gacgtcctga ataaaggtcg
    14221 agatgtacag caggtggctg ttgatacgga acagttcgga gagcataacg cgaatgacgt
    14281 taacgcgatc cggcacggtg atcccggcca gtttctctac cgccagcacg taaggcattt
    14341 cgttaacgca gccgccgagg tattcaatac ggtcggtata cggaatgtag ctgtgccagg
    14401 actggcgctc gcccattttc tccgcaccac ggtggtggta gccgatgtct ggtacgcagt
    14461 cgacaatctc ttcgccgtcg agttgtaaaa cgatacggaa agccccgtgc gccgacgggt
    14521 ggttcggacc gaggttgagg aacatgaagt cctcgttttc ggtgccgcgc ttcatccccc
    14581 actcttccgg tttgaaggtc agggcttcca tctccagatc ctgtttggct ttggtcagct
    14641 caaacggcga gaattcggta gcgcgcgccg ggtaatcttt acgcagcggg tgacctttcc
    14701 aggtttgcgg catcatgatg cggcgcaggt tcgggtgacc gtcgaaagta atgccaaaca
    14761 gatcccaggt ttcacgctca taccagttag cgttcgggaa cagtttggtg aaggtcggta
    14821 cgtgcaggtc gttttctgcc agcgccacct tcagcatgat gtcgcggtta cgatcgatag
    14881 aaatcagatg gtagaaaacg gaaaaatccg cggcaggtaa cccttcgcgg tgtgtgcgca
    14941 gacgttcgtc catgccgtgt aagtcaaaca gcatgacgta aggtttcggc agtttcttta
    15001 agaaatcgcc aacttccagt aattgttcac gcttgatcca cacaacggga accccggtgc
    15061 gagtcgcctg aacagtaaag gcatccggcc caaaacggtt gcgcagttcg ccaatcaccg
    15121 gatcatcaag atgatcgcgg gtctgccagg cgggttcttg cgcggttaag tcggtcatat
    15181 tgttcaccat tgcaaatggt ccgtggtgac tgtcggcgca gtaagcttcg cgaaatagat
    15241 ttaggaataa gcgaagaaaa atccctttgc cgacaggcgc aaattaaatc tcgtcaggtg
    15301 tacgcaggtt ggttacggca atgcgttcac cgcgcttgcg ttcgcgctct gattgcatat
    15361 tggcgcgata aacgccctga tcgccaacca cccaggagag cggacgacgc tctttgccga
    15421 tagattcctg caacagcatc agtgcctgca tgtacgcttc aggacgcggc gggcagcccg
    15481 ggatatacac atcaaccggg atgaatttat cgacgccctg cacaacggaa taaatatcgt
    15541 acataccacc agagttggca caggcaccca ttgagataac ccattttggt tccagcatct
    15601 ggtcatacag acgctgaata accggtgcca ttttggtaaa gcaggttcct gcaaccacca
    15661 tcaggtcagc ctgacgcggc gaagcacgca atacttctgc gccaaaacgc gccacgtcat
    15721 gcaccgcggt aaacgaagtc accatctcaa cgtaacagca ggaaagaccg aagttatacg
    15781 gccaaattga gtttttacga ccccagttaa ccatgtcatt gagcttgccc ataaacacgt
    15841 ttttgttaac ttcttgctcc agagggtcgg ttacgatctc ctgcttttgc agggggtaac
    15901 ggtcgttctc accgttggga tctatgcggg tgagcgtata atccatctta atgcctcgcg
    15961 gttagcgttg acgattagcg atactgttcg tttccgggtt catacgctcg cggcgtgaac
    16021 gcgcgggcgt ccagtccagc gcgccaatac gcaccagata aaccagacct gccagtaaca
    16081 caaaaataaa aattgcagct tccacaaagc ctacccagcc gctttcgcgg atagaggttg
    16141 accatgcgaa cagatacagc gcttcaacgt cgaagataac gaagaacatg gccaccagat
    16201 aaaacttggc ggacaggcgt aagcgggcgg agccgaccga gtcgataccg gattcaaacg
    16261 gcacgttttt cgacctcgcg cgtgcgcgac cgcctaaaaa ccaaccgcct accagcatca
    16321 ggcaacacag gccaatggca acgataagaa agatagcgaa tgcccagtga tgagcgatga
    16381 cttcagtgga tgttgacata ctcattgctt actcatcaaa agtagcgcca gattcactgc
    16441 tcttcacggc agatggacgc cacatcgatt catggggagg aataaaaaaa accttacaat
    16501 cactgtagaa attcttttat acagctaatt gatgtggtct tttactcctt tctataacct
    16561 tttgtca
//
LOCUS       DAZFQD010000119        16084 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.119, whole genome shotgun sequence.
ACCESSION   DAZFQD010000119 DAZFQD010000000
VERSION     DAZFQD010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16084
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.119"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            59..424
                     /gene="yfiL"
                     /locus_tag="ACHKTW_003853"
     CDS             59..424
                     /gene="yfiL"
                     /locus_tag="ACHKTW_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HFO6464788.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVENASQ
                     LHEVWQKGTDENASTIRLN"
     gene            574..1092
                     /gene="yfiR"
                     /locus_tag="ACHKTW_003854"
     CDS             574..1092
                     /gene="yfiR"
                     /locus_tag="ACHKTW_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HFO6464789.1"
                     /translation="MRFSHRLFLLLILLLTRAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSHFSNALQENGTTSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDALSRSGVKVNP
                     DVLMLARKKNDG"
     gene            1082..2308
                     /gene="dgcN"
                     /locus_tag="ACHKTW_003855"
     CDS             1082..2308
                     /gene="dgcN"
                     /locus_tag="ACHKTW_003855"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
                     /protein_id="HFO6464790.1"
                     /translation="MMDNDNSLNKRPTFKRALRNISMTSIFITMMLIWLLLSVTSVLT
                     LKQYAQKNLALTAATMTYSLEAAVVFADGPAATETLAALGQQGQFSTAEVRDKQQNIL
                     ASWHYTRKDPGDTFSNFISHWLFPAPIIQPIRHNGETIGEVRLTARDSSISHFIWFSL
                     AVLTGCILLASGIAITLTRHLHNGLVEALKNITDVVHDVRSNRNFSRRVSEERIAEFH
                     RFALDFNSLLDEMEEWQLRLQAKNAQLLRTALHDPLTGLANRAAFRSGINTLMNNSDA
                     RKTSALLFLDGDNFKYINDTWGHATGDRVLIEIAKRLAEVGGLRHKAYRLGGDEFAMV
                     LYDVQSESEVQQICSALTQIFNLPFDLHNGHQTTMTLSIGYAMTIEHASAEKLQELAD
                     HNMYQAKHQRAEKLVR"
     gene            2324..2806
                     /gene="yfiB"
                     /locus_tag="ACHKTW_003856"
     CDS             2324..2806
                     /gene="yfiB"
                     /locus_tag="ACHKTW_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFO6464791.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAMGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVITTP
                     "
     gene            complement(2883..3230)
                     /gene="rplS"
                     /locus_tag="ACHKTW_003857"
     CDS             complement(2883..3230)
                     /gene="rplS"
                     /locus_tag="ACHKTW_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFO6464792.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(3272..4039)
                     /gene="trmD"
                     /locus_tag="ACHKTW_003858"
     CDS             complement(3272..4039)
                     /gene="trmD"
                     /locus_tag="ACHKTW_003858"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFO6464793.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            complement(4070..4618)
                     /gene="rimM"
                     /locus_tag="ACHKTW_003859"
     CDS             complement(4070..4618)
                     /gene="rimM"
                     /locus_tag="ACHKTW_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFO6464794.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(4637..4885)
                     /gene="rpsP"
                     /locus_tag="ACHKTW_003860"
     CDS             complement(4637..4885)
                     /gene="rpsP"
                     /locus_tag="ACHKTW_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFO6464795.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(5134..6495)
                     /gene="ffh"
                     /locus_tag="ACHKTW_003861"
     CDS             complement(5134..6495)
                     /gene="ffh"
                     /locus_tag="ACHKTW_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFO6464796.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGKFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            6662..7453
                     /gene="ypjD"
                     /locus_tag="ACHKTW_003862"
     CDS             6662..7453
                     /gene="ypjD"
                     /locus_tag="ACHKTW_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HFO6464797.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLMICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLVHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            7474..8760
                     /gene="yfjD"
                     /locus_tag="ACHKTW_003863"
     CDS             7474..8760
                     /gene="yfjD"
                     /locus_tag="ACHKTW_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HFO6464798.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGEYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(8815..9408)
                     /gene="grpE"
                     /locus_tag="ACHKTW_003864"
     CDS             complement(8815..9408)
                     /gene="grpE"
                     /locus_tag="ACHKTW_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFO6464799.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            9531..10409
                     /gene="nadK"
                     /locus_tag="ACHKTW_003865"
     CDS             9531..10409
                     /gene="nadK"
                     /locus_tag="ACHKTW_003865"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HFO6464800.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            10495..12156
                     /gene="recN"
                     /locus_tag="ACHKTW_003866"
     CDS             10495..12156
                     /gene="recN"
                     /locus_tag="ACHKTW_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFO6464801.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALEIARALHQQRQQYAEELAQLITDSMHALSMPHGQ
                     FTIDVKFDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLNKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            12305..12646
                     /gene="bamE"
                     /locus_tag="ACHKTW_003867"
     CDS             12305..12646
                     /gene="bamE"
                     /locus_tag="ACHKTW_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HFO6464802.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(12708..12998)
                     /gene="yfjF"
                     /locus_tag="ACHKTW_003868"
     CDS             complement(12708..12998)
                     /gene="yfjF"
                     /locus_tag="ACHKTW_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417108.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HFO6464803.1"
                     /translation="MPGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(12988..13425)
                     /gene="ratA"
                     /locus_tag="ACHKTW_003869"
     CDS             complement(12988..13425)
                     /gene="ratA"
                     /locus_tag="ACHKTW_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HFO6464804.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELASNMVQAFTVRAKEVYSAR"
     gene            13596..14078
                     /gene="smpB"
                     /locus_tag="ACHKTW_003870"
     CDS             13596..14078
                     /gene="smpB"
                     /locus_tag="ACHKTW_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFO6464805.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            14293..14655
                     /gene="ssrA"
                     /locus_tag="ACHKTW_003871"
     tmRNA           14293..14655
                     /gene="ssrA"
                     /locus_tag="ACHKTW_003871"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(14816..16084)
                     /locus_tag="ACHKTW_003872"
                     /pseudo
     CDS             complement(14816..16084)
                     /locus_tag="ACHKTW_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417134.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
BASE COUNT         4035 a         4273 c         3790 g         3986 t
ORIGIN      
        1 agataaattt acaccccctt tgaaaacaat tcgctatgct ttgaaaaagg agaaagaaat
       61 gatgaaaaag tttatcgccc ccttgttggc tttactggtt agcggatgcc agattgatcc
      121 ttatactcac gcgccaacct tgaccagcac cgactggtat gatgttggta tggaagatgc
      181 gatatcgggc agcgccataa aagatgacga tgcatttagc gattcacagg cggatcgcgg
      241 tctatacctt aaaggatatg ccgaaggaca aaagaaaact tgccagaccg attttactta
      301 tgcccgggga ctttccggta aaagctttcc tgccagctgt aacaatgttg aaaatgccag
      361 ccaactgcat gaagtttggc aaaaagggac tgatgaaaat gccagcacaa tacgattaaa
      421 ttaattaaaa ccccacaaat aaattaaaca taagatttta ctcattgtgt tttatttctc
      481 acattgatga cgatcgcatg aaaaaatgat attttaatga gcattctgaa aaaatatcga
      541 tgcatttcga gcgaagatgg tggggatccc tgaatgcgtt tttctcaccg actgttctta
      601 ctgctgattc tcctgctgac gagagcacca atactcgctc aggaaccttc tgacgttgcg
      661 aaaaacgtgc gtatgatggt ttccggtatt gtcagttaca cccgctggcc tgcgctatca
      721 gggccaccaa agttatgtat cttttcatct tcacatttta gtaacgccct acaagagaat
      781 ggcacgacat ctttgcctta tctacctgtc atcattcaca ctcaacaaga ggcgatgatt
      841 tcaggctgca atggttttta ttttggcaac gagtcaccga cattccagat ggaattaacc
      901 gaacaatatc cgtcaaaggc gttattatta attgccgaac aaaataccga gtgcattatt
      961 ggtagcgcct tttgtctgat catccacaat aatgacgtca gatttgccgt aaacctggat
     1021 gccttatcgc gtagcggtgt aaaggtcaac ccggatgtct taatgctcgc acggaagaaa
     1081 aatgatggat aacgataatt ctcttaataa gcgccccacg tttaaaagag cattacgcaa
     1141 catcagtatg accagcatat ttatcactat gatgctgatc tggttgctgc tttccgtgac
     1201 ctcggtgctg accctgaaac agtacgcgca aaaaaacctg gcactgacag cagcaacaat
     1261 gacttacagt ctggaagcag ctgtcgtttt tgccgatggc cctgcagcaa ctgaaacact
     1321 ggcagcgctg ggccagcaag ggcaattttc aactgcagaa gtacgtgata agcagcaaaa
     1381 tattctggca tcctggcatt acacccgtaa ggatccaggc gatactttca gcaatttcat
     1441 aagccactgg ctcttccctg cccccatcat tcagccgatt cgtcacaatg gcgaaaccat
     1501 tggcgaagta cgcttaaccg ctcgcgacag ttcaatcagc cattttatct ggttttcgct
     1561 cgccgtactg accggttgta ttctgctggc atcaggaatc gcaattaccc tcacccgcca
     1621 tttgcacaat ggcctggtgg aagcactgaa aaatatcacc gatgtcgtac atgatgtgcg
     1681 ttccaaccgc aatttttccc gacgagtttc ggaagaacgt attgctgagt ttcaccgctt
     1741 cgctctcgac ttcaacagtc tgctggatga aatggaagag tggcagcttc gtttacaggc
     1801 taaaaatgcg cagcttctac gtaccgcgct acatgaccca ttaaccgggc tggctaaccg
     1861 cgcagcgttt cgtagcggca tcaacacgtt gatgaacaat tccgatgccc gaaaaacgtc
     1921 ggcgttacta tttcttgatg gcgataattt caaatacatc aatgatacct ggggtcatgc
     1981 gacgggcgat agagtcttga ttgaaatcgc aaaacggtta gctgaagttg gcgggctgcg
     2041 acataaagca taccgcctgg gcggcgatga attcgctatg gtgctctatg atgtacagtc
     2101 agaatctgaa gtgcagcaga tatgctcagc actgacacaa atctttaatc tcccgtttga
     2161 tcttcataat ggccatcaga ccaccatgac attaagcatt ggttacgcga tgaccattga
     2221 gcacgcctct gcggaaaaat tacaagagct tgccgatcac aatatgtatc aggccaaaca
     2281 ccagcgtgcc gaaaagctgg tgagataaca aggatatatc gatatgataa agcacctggt
     2341 agcacccctg attttcacct cactaatact gactggctgc cagtcccctc agggaaagtt
     2401 tactcctgag caagtcgccg ctatgcaatc ttatggattt actgaatccg ccggcgactg
     2461 gtcgctgggc ttatcagatg ccattctgtt cgcaaaaaat gactacaaat tgctcccgga
     2521 aagccagcaa cagatccaaa ccatggcagc caaattggcc tcgacagggc taacacatgc
     2581 ccgtatggat ggacacaccg ataactatgg tgaagacagt tacaacgaag gcttatcatt
     2641 gaaacgggcg aatgtcgtgg ccgatgcatg ggctatgggt ggacaaattc cacgcagcaa
     2701 tctcaccaca cagggtttag gaaaaaaata tcccatagcc agtaacaaga ccgcccaggg
     2761 ccgcgccgag aaccgccgcg tcgcagtggt gattacaacc ccttaaaaca cgagcaaaca
     2821 gacaaaaaaa gggccagcca attggccagc ccttcttaac aggatgtcgc ttaagcgaaa
     2881 tcttagttaa gacgctcttt gatacgagca gccttaccag tacgctcacg caggtagtac
     2941 agtttagctt tacgaacagc accacgacgt ttgacagaaa tgctgtcaac taccggagag
     3001 tgagtctgga agacacgctc aacgccttcg ccgttggaaa ttttacgaac agtgaatgca
     3061 gagtgcagac cgcggttacg aatagcgata accacgccct cgaatgcctg cagacgtttt
     3121 ttggaacctt caacaaccca tactttcact tccacggtat cacccggacg gaaggaaggt
     3181 acgtcctgct tcatctgctc ttgttcaagt tgcttaataa tgttgctcat aatttaatct
     3241 cttatcctgg gtaaactgat atctcggggg cttacgccat cccatcatgt ttatgttgct
     3301 gttgtgcgtg ttccgttttg aactccgcca gcaaccttgc ttgctcttca gtcagagcca
     3361 ggttttccag aagttcaggt cttctaagcc aggtacggcc cagcgactgt ttcaaacgcc
     3421 agcgacgtat ctcggcatgg ttgcccgaca gtaacactgg cggaacttcc atcccttcta
     3481 acacctcagg ccgcgtatag tgcgggcaat ccagcaatcc ttcagcaaag gaatcttccg
     3541 ttgccgaggc ttcatgtccc agtactcccg gaataaaccg ggaaacggag tcaatcagcg
     3601 tcattgctgg taactcacca ccactgagaa cgtaatcgcc gattgaccat tcttcgtcaa
     3661 tttcggtttg gatcacgcgc tcatctatac cttcgtagcg accgcacacc agaatcaatt
     3721 tttgattcgt tgccagttcg ctgacgcccg cttgatcaag cttgcgtccc tgtggtgaca
     3781 gataaatcac ctttgcgcct tcacccgccg cggcttttgc tgcatgaatg gcgtcccgca
     3841 agggttgcac catcattaac atccccggtc cgccgccgta aggacgatcg tccacggtac
     3901 ggtgccggtc atgcgtgaag tcgcgaggac tccagctctg gatgctcagc aggccatttt
     3961 taactgcccg gccagttacc ccgtaatcgg taattgcgcg gaacatttca ggaaacaggc
     4021 taattatgcc aatccacata gcgccgtctt ttaccgttta tccggtggtt taaaaaccag
     4081 gatcccaatc tacttcgatt gaacgagtag tgagatcgac tttcttgata acctgcccat
     4141 cgaggaacgg tacgagacgt tccttgatac caaacgcatc tttcaggttt gccttaatga
     4201 cgagaacgtc attagatccg gtttccatca tatcgacgac tttaccgaga tcgtagcctt
     4261 cagtggttac tacctggcag cccatcaggt ctttccagta gtagtccccc tcttcaagct
     4321 gaggcagctg cgatgaatcc acgacaattt cacaattcgt cagcaggttc gccgcatcac
     4381 gatcgtcaac gcctttcagc ttgatgatca tgtcctgatt gtggtgcttc cagctttcca
     4441 gctggacttg ctgccactga cccgccttct ggataaacca aggctgatag tcaaaaatgc
     4501 tttcggcgtc ttcggtggaa gaaaacactc tgagccaccc acgaataccg taagacgaac
     4561 ccatttttcc caaaacgatg ggatcaacag gtgcttgcgc ggtgagttgt ttgctcatca
     4621 tgaccaccgt gacagattaa gctgctttgt ttacttcttt gatcagcgca gcaacgcgat
     4681 cagaaatagt tgcgccctgg ccaacccagt gagcgatgcg atccagatcc aggcgagtgc
     4741 cttcttcttt ttcgctagcg attgggttga agaaaccaac gcgctcgatg aagcgaccgt
     4801 tgcgtgcatt acggctgtca gcgacaacaa cctggtagaa cggacgcttt ttagcgccgt
     4861 gacgtgctaa acgaatagtt accataacat cctcttgtgt gaataaaaca accggacccc
     4921 atcgaggaac ggagtccggt gtcatattaa aagcccgaaa attttactca tttttgcggg
     4981 aattgcaatc aacagttgct aactctgctg taaaaggccg tcggcggtgc agccagtttg
     5041 gtgccggagt gcgcgcagtc accggagcgt acacgcagta cgtgaggatg acgagcacat
     5101 cccggtgcca aaatggcaaa caagccaggc cgattagcga ccagggaagc ctgggggcat
     5161 catacccttc atgcttctca tcatcttcgc cattccgcct ttcttcattt tcttcatcat
     5221 gcgctgcatg tcgtcgaact gcttcagaag gcggttcacg tcctgcacct gcataccgca
     5281 accggcagcg atacgacgct tacgcgaacc tttgatgatt tctggcttag cgcgctcttt
     5341 catcgtcatc gagttgatga tggcttccat acgcaccagc actttatcgt ccatctgcga
     5401 cttaacatta tccgggatct gccccatgcc cggcagcttg cccatcaggc tagccatgcc
     5461 gcccatattt ttcatctggc gcagttgctc aagaaaatcg ttgagatcga agccgtcgcc
     5521 ttttttcagc ttgctggcta atttctctgc ctgcgcgcgg tcaactttgc tttcgatatc
     5581 ttcgatcagc gacagtacgt cgcccatgcc gagaatacgc gacgcgatgc ggtccggatg
     5641 gaacggctcc agcgcctcag ttttctcgcc aacaccgaga aacttgatcg gtttgccagt
     5701 gatgtgacga atagagagcg ccgcaccgcc gcgggcatcg ccgtccactt tggtcaacac
     5761 tacgccggta agcggtaacg cttcattgaa tgcttttgcc gtattggccg catcctgacc
     5821 ggtcatggcg tcaaccacaa acagggtttc aaccgggtta atcgacgcat ggacttgttt
     5881 gatctcgtcc atcatcgctt cgtcaacgtg cagacgacca gcggtatcca ccagcagcac
     5941 gtcgtagaat ttcagtttgg cttctttcag cgccgcgtta acgatatcta ccggcttctg
     6001 accaacatca gaagggaaga aatcaacgcc cacctgctct gccagcgtct caagctgttt
     6061 gattgccgcc gggcgataaa cgtcggcaga aaccaccagc actttcttct tgtgcttctc
     6121 gcgcaggaac ttaccgagtt taccaacgct ggttgtttta ccggcacctt gcaggcccgc
     6181 catcagcacg accgcaggcg gttgcgccgc caggttcagg gtctggttct cttcgcccat
     6241 cgccgcaacc agttcgttac ggacgatttt gacgaactcc tgccccggcg tcaggctctt
     6301 attaacttca tgaccaaccg ctttctcttt tacgcgattg ataaactcac gcactaccgg
     6361 cagagcgacg tccgcctcca gcagcgccat gcgcacttcg cgcagcgtat cttttacgtt
     6421 gtcttcagtg aggcgtccac ggccactgat attgcgcagc gtgcgcgaca aacgatcggt
     6481 taaattatca aacattgtct ctcgcctggg gtggaaacgg ttggccgcaa tcgcgacaca
     6541 tcatcagtat tttgccgcag tataacatga aggcgtcttt gttgttatgc aacggttgga
     6601 gcagcgttca cctgacgcta tactgcttct ctttcttatt gctcaaactg tcgacatcac
     6661 tatgcccgtt tttgctctgc tcgcgcttgt cgcctactcc gtcagtcttg cgctgattgt
     6721 tcccggtctg ctgcaaaaaa acggcggctg gcggcgcatg gctattattt ctgcggtcat
     6781 tgcgctggtc tgccacgcaa tcgctctgga agcccgcatc ctgcccgacg gtgatagcgg
     6841 acaaaacctc agcctgctga acgttggttc attggtcagt ttgatgatct gtacggtaat
     6901 gaccattgtg gcttctcgca atcgtggctg gttgctgcta cccattgtct atgcctttgc
     6961 gcttatcaac ctggcgctgg cgaccttcat gcccaatgaa tacatcaccc atctggaagc
     7021 aacgcctggg atgctggtgc acattggctt atcgctcttt tcctatgcca cgctgataat
     7081 tgccgccctg tacgcgctac aactggcgtg gattgattac caactgaaga acaagaagct
     7141 ggcgtttaac caagaaatgc cgccattgat gagtatcgag cgtaaaatgt tccacatcac
     7201 gcagattggc gtggtgctac taacgctcac gctttgcact ggcctgttct acatgcacaa
     7261 cttatttagc atggaaaata tcgacaaggc cgtgctctct atcgtggcgt ggtttgtcta
     7321 tattgtgctg ctgtggggac attatcatga aggatggcgt ggacgccgcg tcgtctggtt
     7381 taacgttgcg ggtgcagtca tcctgacact ggcctacttc ggcagccgaa ttgtccagca
     7441 gttaatcagc taaacccaga aaaggagttt cccctggaac acatttctac tactacgttg
     7501 atcattattc tgatcatcat ggtggtcatt tcagcctatt tttccgggtc cgaaaccgga
     7561 atgatgaccc tcaaccgcta tcgtctgcga catatggcga aacagggtaa tcgctcggcc
     7621 aaacgcgtcg aaaaattgct gcgtaagcca gaccgcctga taagcctggt gttaatcggc
     7681 aataacctgg tcaatattct tgcctccgcg ctcggcacta ttgttgggat gcgtttgtac
     7741 ggcgatgcgg gcgtggcaat tgcgactggt gtgctgactt ttgtcgtact ggtttttgct
     7801 gaggtattgc cgaaaaccat tgccgcgctg tacccggaaa aagtcgctta tccgagtagt
     7861 tttctgctgg ctccgctgca aattttgatg atgccgctgg tctggttgct gaatgctatc
     7921 acccgtatgc tgatgcgcat gatgggtatc aaaaccgata tcgtggttag cggctctttg
     7981 agcaaagaag agttgcgcac tatcgtgcac gaatcacgct cacaaatttc ccgtcgcaat
     8041 caggatatgc tactgtctgt gctcgatctg gaaaagatga ccgttgatga catcatggtg
     8101 ccgcgcagtg aaattatcgg tattgatatc aacgatgact ggaaatcgat tctgcgccaa
     8161 ctctcccact cacctcacgg gcgcatcgtg ctctaccgtg attcgctgga cgacgccatc
     8221 agtatgctgc gagtacgtga agcctggcgg ttgatgtcgg agaaaaaaga gttcaccaaa
     8281 gaaaccatgc tgcgcgccgc ggacgagatc tattttgtgc cggaaggtac gccgctcagc
     8341 acgcagttgg taaagtttca gcgcaacaaa aagaaagtcg gcctggtcgt caacgagtat
     8401 ggagacattc aggggctggt gacggttgaa gatattctgg aagagattgt cggcgatttc
     8461 actacgtcga tgtcgccaac acttgccgaa gaggtcacgc cgcaaaacga cggttcggtg
     8521 attatcgatg gcaccgccaa cgtgcgggaa atcaacaaag cctttaactg gcatctaccg
     8581 gaagatgatg cccgcacggt taatggcgtc attcttgagg cactggaaga gatccctgtc
     8641 gcaggcaccc gcgtgcgtat tggcgagtac gatatagata ttctcgacgt acaggacaat
     8701 atgattaagc aggtaaaagt ttttcctgtg aaaccgctgc gcgagagtgt ggcggagtaa
     8761 cgaaaacggc cctgcattcg catgcagggc cgtgaattat tacgaaaaca gaaattacgc
     8821 ttttgctttc gctacagtta ccatcgccgc acgaatcgta cgaccattca gcgtataacc
     8881 cttctgcata atgcccagta cgttacctgg cgcaacgtca tcagattcca ccattgcgat
     8941 agcctgatgc acattcgggt ccagcggcac gttagtttcg gcgatcactt caacaccaaa
     9001 cttacgcaca acatccagca tcgacttcag cgtcagctca atgccttcaa ccatcgcaga
     9061 catatccggg ttagctttat cagccacttc cagcgcacga tccaggctat caatcaccgg
     9121 cagcaattcg ttgatgaatt tctccagcgc gaatttgtgg gctttttcaa tatccagttc
     9181 agtacgtcga cgcaggtttt ccatttcggc ttttacacgc aaaatgccgt cacgttcacg
     9241 ggtctgggct tcagccagct gagcttcgag attcgcaatt ttttcatcgc gcggatccac
     9301 ctgctcagca gaagcttctg gctcaactgc ctcaatctct tcgtgctgat ccatgataat
     9361 ttcttccggg gcttgcccct caggcgtttt ctgttcttta ctactcatga atttctccgc
     9421 gtttttttcg cattcatctc gctaacttcg cttattatgg ggatcagttt cagggtttca
     9481 agggaagcac tcacattgtc atcaatcttc gcaacaagga cctcggaaaa atgaataatc
     9541 atttcaagtg tattggcatt gtgggacacc cacggcaccc cactgcactg acaacacatg
     9601 aaatgctcta ccgctggctg tgcacaaaag gttacgaggt catcgttgag caacaaatcg
     9661 ctcacgaact gcaactgaag aatgtgaaaa ctggcacgct cgcggagatt gggcaactag
     9721 ctgatctcgc ggtagtcgtt ggtggcgacg gtaatatgct gggcgcggca cgcacactcg
     9781 cccgttacga tattaaagtt attggaatca accgtggcaa cctgggtttc ctgactgacc
     9841 ttgaccccga taacgcccag caacagttag ccgatgtgct ggaaggccac tacatcagcg
     9901 agaaacgttt tttgctggaa gcgcaagtct gtcagcaaga ttgccagaaa cgcatcagca
     9961 ccgcgataaa tgaagtggtg cttcatccag gcaaagtggc gcatatgatt gagttcgaag
    10021 tgtatatcga cgagatcttt gcgttttctc agcgatctga tggactaatt atttcgacgc
    10081 caacaggctc caccgcctat tccctctctg caggcggtcc tattctgacc ccctctctgg
    10141 atgcgattac cctggtgccc atgttcccgc atacgttgtc agcacgacca ctggtcataa
    10201 acagcagcag cacgatccgt ctgcgttttt cgcatcgccg taacgacctg gaaatcagtt
    10261 gcgacagcca gatagcactg ccgattcagg aaggtgaaga tgtcctgatt cgtcgctgtg
    10321 attaccatct gaatctgatt catccgaaag attacagtta tttcaacaca ttaagcacca
    10381 agctcggctg gtcaaaaaaa ttattctaat tttacgccag cctctttact gtatataaaa
    10441 ccagtttata ctgtacacaa taacagtaat ggtttttcat acaggaaaac gactatgttg
    10501 gcacaactga ccatcagcaa ctttgctatc gttcgtgagc ttgagattga ttttcatagc
    10561 ggcatgaccg taataactgg cgagaccggc gcgggtaaat ctattgcaat agatgccctc
    10621 ggtctttgtc tcggtggtcg cgctgaagcc gacatggtgc gtaccggcgc tgctcgcgct
    10681 gacctgtgcg cccgtttttc tctgaaagat acgccagcgg ctctgcgctg gctggaagaa
    10741 aatcagcttg aagacgggca tgaatgtttg cttcgtcgag tgatcagcag cgatggtcgc
    10801 tcccgtggtt tcatcaacgg tacagctgtt cctctgtcac aactgcgcga actgggtcag
    10861 ttgctgattc agatccatgg tcagcacgct catcaattac tcaccaaacc tgagcaccaa
    10921 aaattcctgc ttgatggcta tgccaatgaa acctctctac tgcaggaaat gaccgcacgt
    10981 tatcagttgt ggcatcaaag ctgccgtgac ctcgcgcatc atcaacagtt aagtcaggaa
    11041 cgcgccgccc gtgcggaact gctgcaatac caattaaaag aacttaacga atttaatccg
    11101 cagcccggag agtttgaaca aatcgacgaa gagtacaaac gtctggcgaa cagcggtcaa
    11161 ttgctgacca ccagccagaa tgcattggca ttaatggccg acggtgaaga cgcaaacctg
    11221 caaagtcagc tttacacggc taaacaactg gtgagcgaat tgattggcat ggacagcaaa
    11281 ctgtccggcg tacttgatat gctggaagaa gctaccatcc agattgctga agccagcgat
    11341 gaactgcgcc actactgcga tcgtctggat ctcgatccca accgactatt tgaacttgaa
    11401 cagcgcatct caaaacagat ttcgctggca cgtaaacatc acgtcagccc tgaggcattg
    11461 ccacagtatt accagtcgct actggaagaa cagcagcaac tggacgatca ggccgactca
    11521 caagaaacgc ttgcgctggc ggtaacgaaa catcatcagc aggcactgga aatcgcgcgc
    11581 gcattacacc aacaacgcca gcaatatgca gaagaacttg cacagctgat caccgacagt
    11641 atgcatgcgc tctcaatgcc gcatgggcag tttacgatcg atgttaaatt tgacgagcat
    11701 cacctgggcg ctgacggtgc cgatcgtatt gagtttcggg taaccaccaa cccaggtcag
    11761 ccaatgcagc ctattgccaa agtcgcatcc ggtggtgaat tatcccgcat cgcactggca
    11821 atccaggtca tcacggcgcg taaaatggaa accccggcac tgatttttga tgaagtggat
    11881 gtagggatta gcggtccaac agcggcagtt gtcggcaaac tgctgcgtca actcggcgaa
    11941 tcaactcagg tgatgtgtgt tacccacctg ccacaagtcg cgggatgtgg tcatcaacac
    12001 tattttgtca gcaaagaaac cgatggtgcg atgacagaaa cgcatatgca atccctgaat
    12061 aaaaaagcgc ggttacaaga gctggcgcgc ctgcttggtg gcagtgaagt cacacgtaat
    12121 acactggcga atgcgaaaga actgcttgca gcgtaaactt ttttcctgct tcacggtcag
    12181 agtaaacagc aaaacgccgt aagaccggaa agcaaaaggt tttaaagtga tgaaaggtct
    12241 attatcatcg gcatattaca gatgagccac gtactgctcg ggcccgaaaa ggaatcaaat
    12301 cactatgcgc tgtaaaacgc tgactgctgc agcagcagta ctattgatgt tgaccgcagg
    12361 ctgttccact ctggagcgag tggtttaccg tcctgacatc aaccagggga actatctgac
    12421 cgctaacgac gtatccaaaa tacgtgttgg catgacgcaa caacaagttg cgtacgcatt
    12481 gggtacaccg ctgatgtccg atccatttgg tacgaatacc tggttctatg tcttccgcca
    12541 gcaaccaggt catgaaggtg taacgcagca aacgctgacg ctgaccttta acagtagcgg
    12601 tgtgttgacc aatattgata acaaacctgc gctgagtggt aactaataat aaagttgctc
    12661 tcaaagacgt taaaaaaggt gctcaatgag cacctttttt ctgtctgtta tttattcgct
    12721 gatttttctg ctcgttgcct gcgaagctct ttcggatcgg caatgagagg gcgataaatc
    12781 tccacccgat cgccatcatg cacactatcg cttagttttg ccggacggct gtaaatgccg
    12841 actttgtttt tagttaaatc gatatcggta cgcaattcca gcaagccact ggcgcgaata
    12901 gcttcttcaa ccgtcgcgcc ctcctgcagc gtcacgcgct gcaggtactg cttctcaggt
    12961 agcgcataag ccacctcaac ggcaatttta cctggcactg taaacctctt tcgcacgaac
    13021 cgtaaaagcc tggaccatat tagacgccag ctctttaaac acgcgaccaa aggcgagttc
    13081 aatcaactta ttggtaaact caaagtcgag atgaaactcg atacgacacg cctcctggct
    13141 cagcggcgta aacttccatc caccaatcaa tttcttgaac ggcccatcca ccagattcat
    13201 aagaatactt tggttactgg tcaactggtt gcgggtagta aacgttttgc tgatcccagc
    13261 cttagagaca tctaccgccg cagtcatctg cccaggagtg gactccagaa tccgacttcc
    13321 ggtacaaccc ggcaaaaact gaggataaga ctgaacgtca ttcactaact gatacatttg
    13381 ctccgcgctg tagggtacca gtgcggtccg gctaatctga ggcataacaa tttccatcaa
    13441 caaaaatcca acaaataata tcatttatcc tgctgtaaaa aaaacgctat cccggcgctg
    13501 ggtaacatcg ggttcatgct aagatagagc cttgtccccc gcaggattga tatggggtgt
    13561 tttcgatttc agattaccga tgattcacga cgcttatgac gaagaaaaaa gcacataaac
    13621 ctggttcagc gaccatcgcg cttaacaagc gcgcccgtca cgaatacttt atcgaagaag
    13681 agttcgaagc gggacttgcc ctgcaaggct gggaggttaa atccctgcgc gcaggaaaag
    13741 ccaatatcag cgacagctac gtccttctgc gtgacggaga ggcatttctg tttggcgcta
    13801 acatcacgcc aatggccgtg gcctccacgc atgtggtgtg cgatcctacc cgtacccgca
    13861 agttacttct caaccagcgc gaactggact cattgtacgg tcgcgtcaat cgagaaggct
    13921 ataccgtagt ggcgctctcc ctgtactgga aaaatgcctg gtgcaaagtg aaaatcggcg
    13981 tcgccaaagg taaaaaacag cacgataaac gttcagatat caaagaacgc gaatggcagg
    14041 tggataaagc acgtatcatg aaaaacgccc accgttaaac ctgcactcca attattgacc
    14101 agttcctcac cgcgcctccc tctccggcgg cgcgaatgaa catcttattg gctatcacat
    14161 ccgacacaaa tgttgccatc ccattgctta atcgaataaa aatcaggcta catgggtgct
    14221 aaatctttaa cgataacgcc attgaggctg gtcatggcgc tcataaatct ggtatactta
    14281 cctttacaca ttggggctga ttctggattc gacgggattt gcgaaaccca aggtgcatgc
    14341 cgaggggcgg ttggcctcgt aaaaagccgc aaaaaatagt cgcaaacgac gaaaactacg
    14401 ctttagcagc ttaataacct gcttagagcc ctctctccct agcctccgct cttaggacgg
    14461 ggatcaagag aggtcaaacc caaaagagat cgcgtggaag ccctgcctgg ggttgaagcg
    14521 ttaaaactta atcaggctag tttgttagtg gcgtgtccgt ccgcagctgg caagcgaatg
    14581 taaagactga ctaagcatgt agtaccgagg atgtaggaat ttcggacgcg ggttcaactc
    14641 ccgccagctc caccaatcat gattggacgg tataaggaca acaccaataa aaacaggaag
    14701 ttagcagtct cagcaggaca ccgaccagac ggtgaagaga cataaaagga tacgcaaagg
    14761 agccgcggct cctggtgaca tgaaagccca cagatgtggg ctttttcgtt gatggtcaga
    14821 acgaccagtt cacaccagct accccgttcc acggggattc cacaccggca ccatggctat
    14881 accccacccc aagatgcccg cttaacgtac tgctgaatga ggctttaata cctgcctggt
    14941 atattccacg tctgcccgac aaatcattga cgaaattacc gtcactattc actttcaccc
    15001 ggttatcatc gataaattct ttgcgcacag ccgccttcag ccacggctca acttccatac
    15061 cgttccccag acgcatgttg taactcagcg ttgcacccag ttcacgatat aaactgcggg
    15121 tatcgactga tttcgattcc atgccattgg ataaatgata ttcagggtta tcagcggtga
    15181 acccccgtta acgatgcata cggcgtcagg ttccagttac catcggtaaa tcgcatcccg
    15241 gtttcaatgt gaccgcccag cccgttgctg cgataactgc cgttggcggc tccaccgctg
    15301 ccaaccgtgg agtcgcgagc ttcggtacct gctaatacca ataaattacc gccattttcc
    15361 agcaacatat tggtcgctaa attgccggaa atggaaaaag tgccgtactg gtgagtaccg
    15421 ctgatttcaa taccgttagc cgtgctcgtc tggagagcgg caccgctgtt ctggacgata
    15481 tctgtcgctt tgccattatc gttaactgtc agcgtaccgc cttcattgat ctttgttttt
    15541 attgcctctc cgttagctga aactgtttgt attccgccgt cgttaatcgt tgtctcattc
    15601 gccaccccct cgacgatttg ttcgccgccg gtgagcgtcg tgcctgtcgc agtggctttt
    15661 gttttgacga tctcccgtcc gcccgtattg acctgtgttt tgtcagaaga ggtgtctgac
    15721 tccatggtta acacgccgcc atttgccagc aggatattgt tcgccgcacc ctgctcgatg
    15781 ctgaacgcga cgccatccgc gcgtgttcct gtgacccgcg tcgccctggt ggttgcaacc
    15841 aaagcgtcct ggctactctg ctgtatcccc gttgcgctgc ctttctcccg cacatcgagt
    15901 atgccgccgt cattaagcac cgagttttca gccagaccgc cctcattaac tacctgtgaa
    15961 cacccattaa taatggaacc ttccgctgtc ccgtttgcca taatttgttg taggccagag
    16021 acgatatcgg tattgattgc cttaccataa ttctgaacgg tttgcgtgcc accattgatg
    16081 tgtg
//
LOCUS       DAZFQD010000120        16074 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.120, whole genome shotgun sequence.
ACCESSION   DAZFQD010000120 DAZFQD010000000
VERSION     DAZFQD010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16074
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.120"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..101)
                     /gene="yojO"
                     /locus_tag="ACHKTW_003873"
     CDS             complement(<1..101)
                     /gene="yojO"
                     /locus_tag="ACHKTW_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YojO"
                     /protein_id="HFO6464806.1"
                     /translation="MHTPIGVKPVAGSKEWREAWQKRAFAHISNGYK"
     gene            210..704
                     /gene="napF"
                     /locus_tag="ACHKTW_003874"
     CDS             210..704
                     /gene="napF"
                     /locus_tag="ACHKTW_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HFO6464807.1"
                     /translation="MKIDASRRGILTGRWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGAGGYPSVNFKNNECSFCYDCAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQPQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            694..957
                     /gene="napD"
                     /locus_tag="ACHKTW_003875"
     CDS             694..957
                     /gene="napD"
                     /locus_tag="ACHKTW_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HFO6464808.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            954..3440
                     /gene="napA"
                     /locus_tag="ACHKTW_003876"
     CDS             954..3440
                     /gene="napA"
                     /locus_tag="ACHKTW_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HFO6464809.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNRRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPG
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            3447..4142
                     /gene="napG"
                     /locus_tag="ACHKTW_003877"
     CDS             3447..4142
                     /gene="napG"
                     /locus_tag="ACHKTW_003877"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HFO6464810.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            4129..4992
                     /gene="napH"
                     /locus_tag="ACHKTW_003878"
     CDS             4129..4992
                     /gene="napH"
                     /locus_tag="ACHKTW_003878"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HFO6464811.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILLGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGSLLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AASDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            4989..5438
                     /gene="napB"
                     /locus_tag="ACHKTW_003879"
     CDS             4989..5438
                     /gene="napB"
                     /locus_tag="ACHKTW_003879"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HFO6464812.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQERMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            5448..6050
                     /gene="napC"
                     /locus_tag="ACHKTW_003880"
     CDS             5448..6050
                     /gene="napC"
                     /locus_tag="ACHKTW_003880"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HFO6464813.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSRLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            6063..6686
                     /gene="ccmA"
                     /locus_tag="ACHKTW_003881"
     CDS             6063..6686
                     /gene="ccmA"
                     /locus_tag="ACHKTW_003881"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HFO6464814.1"
                     /translation="MGMLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTT
                     LLRLLTGLSRPDAGEVLWQGQPLHQVRDSYHQNLLWIGHQPGIKTRLTALENLHFYHR
                     DGDAAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAID
                     VNGVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTGAA"
     gene            6683..7345
                     /gene="ccmB"
                     /locus_tag="ACHKTW_003882"
     CDS             6683..7345
                     /gene="ccmB"
                     /locus_tag="ACHKTW_003882"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HFO6464815.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            7387..8124
                     /gene="ccmC"
                     /locus_tag="ACHKTW_003883"
     CDS             7387..8124
                     /gene="ccmC"
                     /locus_tag="ACHKTW_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HFO6464816.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            8121..8330
                     /gene="ccmD"
                     /locus_tag="ACHKTW_003884"
     CDS             8121..8330
                     /gene="ccmD"
                     /locus_tag="ACHKTW_003884"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HFO6464817.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            8327..8806
                     /gene="ccmE"
                     /locus_tag="ACHKTW_003885"
     CDS             8327..8806
                     /gene="ccmE"
                     /locus_tag="ACHKTW_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HFO6464818.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            8803..10746
                     /gene="ccmF"
                     /locus_tag="ACHKTW_003886"
     CDS             8803..10746
                     /gene="ccmF"
                     /locus_tag="ACHKTW_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HFO6464819.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLCGRLDSIYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLAIAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGITFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTRDGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            10743..11300
                     /gene="dsbE"
                     /locus_tag="ACHKTW_003887"
     CDS             10743..11300
                     /gene="dsbE"
                     /locus_tag="ACHKTW_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HFO6464820.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            11297..12349
                     /gene="ccmH"
                     /locus_tag="ACHKTW_003888"
     CDS             11297..12349
                     /gene="ccmH"
                     /locus_tag="ACHKTW_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HFO6464821.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVQEGKRAGYIVYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRSDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(12384..13031)
                     /gene="narP"
                     /locus_tag="ACHKTW_003889"
     CDS             complement(12384..13031)
                     /gene="narP"
                     /locus_tag="ACHKTW_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HFO6464822.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGDGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRAGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            13351..15942
                     /gene="yejO"
                     /locus_tag="ACHKTW_003890"
                     /pseudo
     CDS             13351..15942
                     /gene="yejO"
                     /locus_tag="ACHKTW_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter YejO"
BASE COUNT         3566 a         3930 c         4690 g         3888 t
ORIGIN      
        1 tatttataac catttgaaat gtgagcaaaa gcccgttttt gccacgcttc ccgccactct
       61 tttgatcctg ctacaggttt taccccgatc ggggtatgca tctttgacac atcctttaat
      121 atcttagcgg ctataaaaat ggcttattaa ttatgcggtt tatttggtcg ctctcaattt
      181 tcagagcgcg ttaatgatgg aaggtcaatg tgaagattga tgcatcccgt cggggcatac
      241 tcactggtcg ctggcgcaaa gccagtaacg gtatccgtcc gccctggtcc ggtgatgaat
      301 ctcattttct gacccattgc acccgctgcg acgcctgtat taatgcttgt gaaaacaaca
      361 ttctgcaacg cggcgcgggc ggctatccga gcgttaattt caaaaataac gagtgcagct
      421 tctgttacga ctgcgcgcag gcttgccccg aatcgctatt ttctccgcgc cacaccaggg
      481 cctgggattt gcagttcacc attggggacg cgtgcctggc gtatcagtct gttgaatgcc
      541 gccgctgtca ggatagctgt gagccaatgg caattatctt tcgcccgacg ctctccggga
      601 tctaccagcc gcaacttaat agccaacttt gcaacggatg tggagcatgt gcagccagct
      661 gtccggtatc agccataact gcggagtatc tccatgcaca ctaactggca agtttgcagc
      721 ctggtcgtgc aggccaaaag cgaacgaatt tcagacatca gcacccaact gaacgccttt
      781 cccggctgtg aagttgctgt cagcgacgcg ccgagcggtc agttgattgt ggtggtggaa
      841 gcagaagaca gcgaaacgct gatccaaacc attgagtcag tacgcaacgt agagggcgtg
      901 ctggcggtgt cgctggttta tcaccagcag gaagagcaag gtgaggaaac accatgaaac
      961 tcagtcgtcg tagctttatg aaagctaacg ccgttgcggc cgctgcggcg gctgccggtc
     1021 tcagcgtgcc gggcgttgcc cgcgccgttg ttggtcagca ggaagccatt aaatgggata
     1081 aagcgccgtg ccgtttctgc ggtactggtt gcggcgttct ggtcggaacg cagcaggggc
     1141 gtgtggtggc ctgtcagggc gacccggacg caccggttaa ccgtggcctg aactgcatta
     1201 agggctattt cctgcccaag atcatgtacg gtaaagaccg tttgacgcag ccgctgctgc
     1261 gtatgaaaaa cggtaaatat gacaaagaag gcgaatttac cccaatcacc tgggatcagg
     1321 ccttcgatgt gatggaagag aaattcaaaa ccgccctgaa agaaaaagga ccggaatcga
     1381 tcggtatgtt cggttctggt cagtggacta tctgggaagg ttatgccgcg tccaagctgt
     1441 tcaaagcggg cttccgttcg aacaacatcg acccgaacgc acgtcactgt atggcgtcgg
     1501 cagtagttgg ctttatgcgt acctttggta tggatgagcc gatgggctgc tatgacgaca
     1561 tcgagcaggc tgacgcgttt gtgctgtggg gcgctaacat ggcggagatg cacccgatcc
     1621 tctggtcacg catcactaac cgtcgtctct ctaaccagaa cgtcaccgtg gcggtgcttt
     1681 ctacctacca gcatcgtagc ttcgagctgg cggataacgg catcatcttt acgccgcaat
     1741 ctgacctggt gatcctgaac tacatcgcca actatatcat tcaaaacaat gcgataaatc
     1801 aggacttctt cagcaagcac gttaacctgc gcaaaggggc gacggacatc ggctacggtt
     1861 tacgtccgac ccatccgctg gaaaaagcag cgaagaatcc gggttctgac gcctccgaac
     1921 cgatgagctt tgaagattac aaagccttcg ttgctgagta tacgctggaa aaaaccgctg
     1981 aaatgaccgg cgtgccgaaa gaccagttag aacaactggc gcagctgtat gccgatccga
     2041 acaagaaagt catctcctac tggacgatgg gcttcaacca gcatacccgt ggtgtgtggg
     2101 ccaacaacct ggtctacaac ctgcacctgc tgaccggcaa aatttcccag ccgggttgcg
     2161 gtccgttctc cctgaccggg cagccttctg cgtgtggtac tgcgcgtgaa gtgggcacct
     2221 ttgctcaccg tctgcctgcg gacatggtgg tgactaacga gaaacatcgt gatatctgcg
     2281 agaagaagtg gaatatcccg ggcggcacca ttccggcaaa aatcggtctg cacgcggtgg
     2341 cacaagaccg tgcgctgaaa gacggcaagc tgaatgttta ttggaccatg tgtaccaaca
     2401 acatgcaggc cgggccgaac attaatgaag agcgtatgcc gggctggcgc gatccgcgca
     2461 acttcatcat cgtctccgat ccgtatccga cagtcagtgc gctggccgcc gacttgatcc
     2521 tgccgaccgc aatgtgggta gagaaagagg gcgcttacgg taacgccgaa cgccgtactc
     2581 agttctggcg tcagcaagta caggcaccgg gcgaagcgaa atcggatctc tggcagttag
     2641 tccagttctc ccgccgcttc aaaactgaag aagtatggcc ggaagagctg ctggcgaaga
     2701 aaccggaact gcgtggcaaa acgctgtacg aagtgctgta tgccacaccg gaagtgagca
     2761 aattcccggt atccgaactg gcggaagatc agcttaatga tgaatcccgc gagctgggct
     2821 tctatctgca aaaagggctg ttcgaagagt acgcatggtt tggtcgcggt cacggtcacg
     2881 atctggcacc gttcgatgac taccacaaag cgcgcggtct gcgctggccg gtggtgaacg
     2941 gtaaagaaac gcagtggcgt tacagcgaag gtaacgaccc gtacgtgaaa gcgggcgaag
     3001 gctataagtt ctacggtaaa ccggatggta aagcggtgat cttcgcgctg ccgttcgaac
     3061 cggcggcgga agcaccggat gaagagtacg acctgtggct ctctaccgga cgcgtcctgg
     3121 agcactggca caccggcagt atgactcgcc gtgtgccgga actgcaccgc gccttcccgg
     3181 aagcggtcct gtttattcac ccgctggatg cgaaagcgcg cgatctgcgc cgtggcgaca
     3241 aagtgaaagt ggtttctcgc cgtggcgaag tgatctcgat tgtcgaaacg cgcggtcgta
     3301 accgtccgcc gcagggcctg gtgtacatgc cgttcttcga cgccgcacag ctggttaaca
     3361 aactaacgct ggacgcgacc gatccgctct cgaaagagac ggacttcaag aagtgcgcag
     3421 tcaaactgga gaaggtgtaa gccattatgt cccggtcagc gaaacctcaa aatggtcgcc
     3481 gccgctttct gcgcgatgtt gttcgcacag caggcgggct ggctgccgtg ggtgtggcgc
     3541 tggggttaca acagcaaacc gcacgcgcat ctggcgtgcg gttgcgcccg cccggagcca
     3601 taaacgagaa cgcctttgcc agtgcctgtg tgcgttgcgg tcagtgtgtt caggcttgcc
     3661 cttacgacac cttaaaactg gcgacgctgg cttctggcct gtcggcgggc acgccatatt
     3721 ttgtcgcacg ggatattcct tgcgaaatgt gtgaggacat tccgtgcgcc aaagtgtgcc
     3781 caagcggtgc gctggatcgt gagattgaat cgatcgacga cgcgcggatg gggctggcgg
     3841 tactggtgga ccaggaaaac tgtctcaact ttcaggggct gcgctgcgat gtttgttatc
     3901 gcgaatgccc gaaaattgat gaggccatca ccctggagct ggagcgcaac acgcgtaccg
     3961 gtaagcacgc ccgctttctg ccgacggttc acagcgacgc ctgtaccggt tgcggtaagt
     4021 gcgaaaaagt atgcgtgctg gaacaaccgg caatcaaggt gttaccgctg tcactggcga
     4081 aaggggagtt aggtcaccat taccgcttcg gctggctgga ggggaacaat ggcaaatcgt
     4141 aaacgtgacg ccgggcgcga ggcgctggag aaaaaaggct ggtggcgcag tcaccgttgg
     4201 ctggtgttac gtcgcctttg tcagttcttc gtgctgggga tgtttttgag cggtccgtgg
     4261 tttggtgtgt ggatcttaca cggtaactac agcagtagcc tgttattcga caccgttccg
     4321 ctgaccgatc cgctgatgac gctgcaaagt cttgccagtg gtcatctgcc tgccacggtg
     4381 gcgttgaccg gtgcggtgat tatcaccgtg ctctacgccc tggcaggaaa gcgattattt
     4441 tgcagctggg tctgcccgct gaatccgatt accgacttag cgaactggtt acgcaggcgc
     4501 tttgacctca atcagtctgc gacgatccct cgccacattc gctacgtgct gttggtggtc
     4561 attctgctgg gatcggcctt aactggcacg ctcatctggg aatggatcaa ccccgtatct
     4621 ctgatggggc gcagcctggt aatgggcttc ggcagcggct cgctgcttat tctcgcgctg
     4681 tttttatttg atctactggt cgttgaacac ggctggtgcg ggcacatttg tccggttggc
     4741 gcgctgtatg gcgtgctggg tagcaaaggt gtgattaccg ttgcggccag cgatcggcag
     4801 aaatgtaacc gctgtatgga ttgttttcat gtttgcccgg aaccgcatgt gctacgtgcc
     4861 ccggtgctgg atgaacaaag cccggtgcag gtcaccagcc gcgattgcat gacgtgcggt
     4921 cgctgcgtgg atgtctgttc tgaggatgta tttacaataa ctacacgatg gagttcggga
     4981 gcgaaatcat gaaaagccat gacctgaaga aagcgctgtg tcaatggacg gcgatgctgg
     5041 ccctggtggt aagcggcgcg gtttgggcgg ctaatggcgt cgattttagc cagtcgccgg
     5101 aagtatccgg gacacaggaa ggggccattc ggatgccaaa agagcaggaa cggatgccgc
     5161 tgaactatgt gaatcagccg ccaatgatcc cgcatagcgt tgaaggttat caggtaacga
     5221 ccaataccaa ccgctgcttg cagtgccacg gtgtcgaaag ttatcgcacc actggcgcgc
     5281 cgcgtatcag tcctactcac tttatggaca gcgacggcaa agtgggcgcg gaagtggctc
     5341 cgcgtcgtta tttctgtctg caatgtcacg taccgcaggc cgataccgcg ccaatcgtgg
     5401 ggaatacctt taccccatca aaaggttacg ggaaataaga ggttattatg ggaaattctg
     5461 accgtaagcc tggtctgatt aagcgcctgt ggaaatggtg gcgtaccccc agccgtctgg
     5521 cgctggggac gctgctgttg atcggttttg tcggcggcat cgtcttctgg ggcggcttta
     5581 acactgggat ggaaaaagcc aataccgaag agttctgcat tagctgccac gaaatgcgta
     5641 acacggtgta tcaggaatac atggattccg tgcactacaa caaccgtagc ggcgtccgtg
     5701 cgacctgtcc ggattgtcac gttccgcacg agtttgtgcc gaagatgata cgcaagctca
     5761 aagcaagtaa agagctgtat ggtaaaattt ttggcgttat tgacacgccg cagaaatttg
     5821 aagctcatcg tctgacgatg gcacagaatg agtggcggcg catgaaggac aataactcgc
     5881 aggagtgccg taactgtcac aacttcgagt atatggatac aaccgcccag aaatcggttg
     5941 ccgcgaagat gcatgaccag gcggtgaaag atgggcaaac ctgtattgat tgccataaag
     6001 ggatagcgca caagctgccc gatatgcgtg aagtcgagcc aggtttttaa cagggttatt
     6061 gcgtgggtat gcttgaagcc agagagttac tttgtgagcg ggatgaacga accttattta
     6121 gtggcttgtc atttacgctg aacgcaggag agtgggtaca aatcaccggt agcaacggcg
     6181 cggggaagac aacgcttctc cgtttgctga ctgggttgtc tcgccctgac gctggcgagg
     6241 ttctctggca agggcagccc ttgcatcagg tacgcgacag ctaccatcaa aacctgttat
     6301 ggataggcca tcagccgggg atcaaaaccc ggctgacggc gttagaaaat ctgcactttt
     6361 atcatcgcga tggcgatgcc gcacaatgtc tggaagccct ggcgcaggcc gggcttgccg
     6421 gattcgaaga tattccggta aatcaacttt ctgccgggca acaacgccgc gtcgctttag
     6481 cgcgtctgtg gctgacccgt gccacgttat ggatcctcga cgagcctttt accgcgattg
     6541 acgttaacgg cgtcgatcgt ctgacccagc gtatggcgca gcatacggag caggggggga
     6601 ttgtgattct gactacccac cagccgctca acgttgctga aagtaaaatt cgccgcattt
     6661 cactgacgca aacgggggcc gcatgatgtt ctggcgcatt ttccgtcttg agctgcgcgt
     6721 agcgtttcgc catagcgccg aaatcgccaa cccgctgtgg ttcttcctga ttgtaattac
     6781 cctttttccg ctcagtatcg gtccggagcc gcaactgctg gcgcgtattg caccgggcat
     6841 tatctgggtt gctgcgctgc tttcatcctt gctggcgctg gaacgactgt tccgtgacga
     6901 tttgcaggac ggcagtcttg aacaattgat gttgttgccg ttacccttgc ccgccgttgt
     6961 gctggcgaag gtgatggcgc actggatggt aaccggtctg ccgttactca tcctttcgcc
     7021 actggtagca atgctactgg gaatggatgt ttatggctgg caagtgatgg cgctgacgct
     7081 gctgctggga acgcctacgc ttggctttct cggtgcaccg ggtgtggcgt tgactgtggg
     7141 acttaagcgc ggtggtgtgc tgctcagcat actggtgtta ccgctgacta tcccattact
     7201 catctttgcc accgccgcga tggacgcggc ttccatgcat ttgcccgttg acgggtatct
     7261 ggcaatttta ggcgcgttgc tggcaggcac cgcgacatta agtccttttg cgacggcggc
     7321 agcgttacga atcagcattc aataacgctg gattactgat ttttgttcga gtctggtatc
     7381 gaaactatgt ggaaaacact gcatcaactg gcgatcccac cacggctgta tcaaatctgt
     7441 ggctggttta taccgtggct ggcaattgcc agtgtggtcg tgcttaccgt cggctggatc
     7501 tggggattcg gctttgctcc ggctgattat cagcagggaa atagctaccg cattatctac
     7561 ctgcatgtgc ctgcggcgat ctggtcgatg ggcatttatg catcaatggc ggtggcggct
     7621 tttattggcc ttgtctggca gatgaaaatg gccaacctgg cggtggcggc gatggccccc
     7681 attggtgccg tgtttacctt tattgccctg gttaccggct ctgcatgggg aaaaccgatg
     7741 tggggcacct ggtgggtatg ggatgcacgt ctgacttctg aactggtgct gctgtttttg
     7801 tatgtgggtg tgattgccct gtggcacgcc ttcgacgacc gccgtctggc gggccgtgcg
     7861 gcaggtatcc tggtgctgat tggcgtggtg aatctgccga ttattcatta ctccgtggag
     7921 tggtggaaca ccctgcatca gggatcaacg cggatgcagc aaagtatcga tccggcgatg
     7981 cgttcgccgc tgcgctggtc gatttttggc ttcctgctgc tgtctgccac gctgacgctg
     8041 atgcggatgc gtaatttgat tttgctgatg gaaaaacgcc gtccgtgggt gagtgaactg
     8101 atactgaaaa gaggccgtaa atgacccctg catttgcttc ctggaatgaa tttttcgcaa
     8161 tgggcggtta cgcctttttt gtctggctgg cggtggtgat gaccgttatt ccgctggtgg
     8221 ttttggtcgt gcactcggtg atgcagcatc gcgcaattct gcgtggtgtg gcgcaacagc
     8281 gggcgcgtga ggcgcgttta cgtgctgcgc aacagcagga ggctgcatga atattcgccg
     8341 taaaaaccgc ttgtggattg cctgtgccgt gttggcaggg ctggcgctga ctatcggtct
     8401 ggtgctatat gcgctgcgct cgaatatcga tctcttttat acgccggggg aaattctcta
     8461 cggcaagcgt gaaactcagc aaatgccgga agtcgggcag cgtctgcgcg tcggcgggat
     8521 ggtgatgccg ggtagcgtgc agcgcgatcc caattcgcta aaagtaactt tcaccattta
     8581 cgacgctgaa ggttcagtag atgtctctta cgaaggcatt ttgccggatc tgttccgtga
     8641 agggcagggc gttgtggtgc agggcgagct ggaaaaaggc aatcatatcc tcgcgaaaga
     8701 agtgctggcg aaacacgacg aaaactacac gccgccagaa gttgagaaag cgatggaagc
     8761 taaccaccgt cgcccggcga gtgtttataa ggacccagca tcatgatgcc ggaaattgga
     8821 aacgggctgc tgtgcctggc gttagggatt gcgctgctgc tgtccgtgta tccgctatgg
     8881 ggcgtggcgc gcggagatgc gcgcatgatg gcgtcttccc gcttgtttgc ctggctgctg
     8941 tttatgtctg tggctggcgc atttctggtg ctggtcaatg ctttcgtggt caacgacttt
     9001 accgtcacct atgttgccag caactccaat acccagcttc cggtgtggta tcgcgtggcg
     9061 gctacctggg gcgcgcatga aggctcgcta ctgctgtggg tgctgctgat gagcggctgg
     9121 acctttgcgg tggcgatttt tagtcagcgt attccgctgg atattgtggc ccgcgtactg
     9181 gcgataatgg ggatggtcag cgtcggcttt ttgctgttca ttctctttac ctctaaccca
     9241 ttctcacgca cgctgccgaa cttcccgatt gaagggcgcg atcttaaccc gctgttacag
     9301 gatccggggc tgatcttcca tccgcctctg ctttatatgg ggtacgtggg tttctcggtg
     9361 gcgtttgctt ttgccattgc ttctttgttg tgcgggcgtc tggacagcat ttatgcgcgt
     9421 tttactcgtc cgtggacgct ggcggcgtgg atcttcttga cgctcggcat tgtgctcggt
     9481 tccgcatggg cctattacga actcggctgg ggcggctggt ggttctggga tccggtagaa
     9541 aacgcctcgt ttatgccgtg gctggtgggg actgcgctga tgcactcact ggcggtcact
     9601 gaacaacgcg ccagcttcaa agcgtggaca ttactgctgg caatcagtgc cttctcgttg
     9661 tgtctgctgg ggactttcct ggtgcgttcc ggcgtgctgg tatcggtaca cgcgtttgcc
     9721 tctgatccgg cacgcggtat gtttatcctc gcctttatgg tactggtgat tggtggttcg
     9781 ctgctgctgt ttgccgcgcg tggacacaaa gttcgctcac gcgtaaacaa tgcgctgtgg
     9841 tcgcgggaat ctctgctgtt agcgaacaac gttttgctgg tcgccgcgat gctggtggta
     9901 ttactgggga cgctgctgcc gctggtgcac aagcaactgg gactgggcag tatttcgatt
     9961 ggcgaaccgt tcttcaacac catgtttacc tggctgatgg tgccgtttgc gctgctgctt
    10021 ggtgtcggtc ctctggtgcg ctgggggcgc gatcgcccac gtaaaatccg caatttattg
    10081 attatcgcct tcatctcaac gctggtgctg tcgctgctgc tgccgtggct gttcgaaagc
    10141 aaagttgtgg cgatgacggt gctcggcctg gcaatggcct gctggattgc tgtgctggca
    10201 attgcggaag ctgcgctgcg tatttcacgt ggcacgaaaa ccaccttcag ttattgggga
    10261 atggtggcgg ctcacctggg gctggcagtg acaattgttg gtattacctt tagccagaac
    10321 tatagcgttg agcgtgatgt gcgcatgaag tccggcgata gcgtcgatat tcatgaatat
    10381 cgcttcacct tccgtgatgt caaagaggtg actggcccga actggcgtgg cggtgtggcg
    10441 actatcggcg taacgcgcga tggcaagccg gaaacggtgc tgtatgcgga aaaacgttat
    10501 tacaacactg ccgggtcgat gatgaccgaa gcggcaattg acggcggcat cacgcgtgac
    10561 ctgtacgccg cgctcggtga agagctggaa aacggcgcgt gggccgtgcg tctttactac
    10621 aaaccatttg ttcgctggat ttgggcgggc gggctgatga tggcgttggg cggactgctg
    10681 tgtctgtttg atcctcgcta tcgtaagcgc gtgagtccgc aaaaaactgc gccggaggcc
    10741 gtatgaagcg caaagtattg ttaattccgt tgattatctt cctggcgatt gccgcggcgc
    10801 tgctgtggca gctggcgcgt aatgccgaag gggatgatcc gaccaatctg gaatcggcgc
    10861 tcattggcaa gcctgtgccg aagtttcgtc tcgaatcact ggacaatccg gggcagtttt
    10921 atcaggcgga tgtgctgact cagggcaaac cagtgctgct taacgtctgg gcgacctggt
    10981 gtccgacctg ccgtgcggaa catcaatatc tgaatcagct ttcagcgcag ggcattcgcg
    11041 tggtcggcat gaactataaa gacgatcgcc agaaggcaat cagctggctg aaagagctgg
    11101 gcaatcctta cgcgctaagc ctgtttgatg gcgacggcat gttagggctg gatctcggtg
    11161 tctatggcgc gccagaaacc ttccttattg acggcaacgg tatcattcgc tatcgccatg
    11221 cgggcgatct caatcctcgc gtctgggaag aagagatcaa gccgctgtgg gagaaataca
    11281 gtaaggaggc cgcacaatga ggtttttatt gggcgtgctg atgctgatga tctccggctc
    11341 agcactggcg accatcgacg tgttgcagtt taaagatgaa gcacaggaac aacagttccg
    11401 tcagctcact gaagaactgc gctgcccgaa atgccagaac aacagcattg ccgattccaa
    11461 ctcgatgatt gccaccgacc tgcgtcagaa agtgtatgaa ctgatgcagg aaggtaaaag
    11521 taaaaaagag attgtcgatt atatggtggc gcgttacggc aacttcgtca cttacgatcc
    11581 gccgttaacg ccgctgaccg tgctgctgtg ggtgctgccg gtagtggcta ttggcattgg
    11641 cggttgggtc atatacgccc gctcgcggcg tcgggtacgc gtagtgccgg aagcgtttcc
    11701 tgaacaaagc gtgcaggaag gtaagcgtgc cggatatatt gtttatctgc cgggtattgt
    11761 ggtggcgtta attgtggctg gcgtcagcta ctaccagact ggcaattatc agcaggtgaa
    11821 aatctggcag caggccacgg cacaggctcc ggcgttgctg gacagggcgc tggatccgaa
    11881 agccgatccg ctcaacgaag aagagatgtc gcgtcttgcg ctggggatgc gtactcaact
    11941 gcaaaaaaat ccgggagata tagaaggctg gattatgttg ggccgcgttg gcatggcgct
    12001 gggtaacgct agtatcgcca ccgatgcata cgctactgcg tatcgcctcg atccgaaaaa
    12061 cagcgatgcc gcactgggtt atgctgaagc gttgacacgt tcatctgatc ccaacgacaa
    12121 ccgcctcggt ggtgaactgc tgcgccagtt ggtgagaagc gaccacagca atatccgtgt
    12181 gttaagcatg tatgcgttta atgcctttga gcagcagcga tttggcgaag ccgttgccgc
    12241 gtgggagatg atgttgaaac tcttacctgc caacgatact cgccgtgcgg tgattgaacg
    12301 tagtatcgcg caggcgatgc aacatttgtc gccgcaggag agtaaataaa aagaggcccg
    12361 atggtgatgc catcgggcta tttttattgt gccccgcgtt gttgcaggaa cagaatggtg
    12421 gctgccacgc gtgagcggac attgagtttg cgcagcagat tgcgaatatg tacttttact
    12481 gtctgctctg aaatattcaa caccgaggca atctgtttat ttgacagccc ctgtgccagc
    12541 tcgtgcagaa catccagctc gcgctccgtc agcacgctga agggatcttc ttccgcgcca
    12601 aacatttcac gttcacgtaa gtactgattg acgcgttcgc taaagacttt gctgcctttc
    12661 gctccagcac gaattgcttc cagcaatact tccgggtcgc tgtctttcaa cagataaccg
    12721 tctgcgcctg cgtctatcag tgcaaagaca tcgctggagg catctgatac ggtcaggata
    12781 ataatttgcg cggtaacgcc atccctgcgc aaggcattga gggtatccag gccactcata
    12841 cctttcatat tgagatccag caagatcacg tcgatatcca gtctattcgc cagatcgatc
    12901 gcgctcgcgc cgtcgcccgc ttcggcgacc acttcaaagc caggatcaag ctccagtaac
    12961 tgacgaacac cgcgtcgcat aagtggatga tcatccacaa tcatcacctg aaaaggcgtt
    13021 gcttcaggca tagtagtctc ctgaggtttt attagaatca ttatgtttga gcgtagcgtt
    13081 ggtaacgcta ccaggcgcgt gaatgttacc ccattttatg tatggacatg tagtgaaaac
    13141 tacaaccttg tcgttcgctt tggcatcatt ttcattataa agacggaatt ttgcatcagc
    13201 ttctcatcaa gaaaaacaac taaaaaatca atcacaatac ttgtgcattt ctttaaacag
    13261 tttttaaaaa ctgactccgg gtatggagct atgggtattt tccgtaccca atgcttttaa
    13321 cagcaattaa tttcatagga tgaaagctca attcatcaat ctggttctgt ttctctttgt
    13381 cgttccgcaa tatctgttct ggtggctaca gcgttatatt cacccatagc attggcatca
    13441 acagttgagt atggtgagac agttgatggt gttgtcctgg aaaaagatat ccagctggtt
    13501 tatgggaccg ccaataatac gaaaatcaat cctggcggag aacagcatat aaaagaattt
    13561 ggtgtaagta gtaatactga aattaaagga gggtatcagt acattgaaat gaatggtacc
    13621 gcagaatact cagtattaaa tgacggttat caaattgttc aaatgggtgg cgcggcaaac
    13681 cagactaccc tcaataatgg tgtgctacag gtttatggcg cagcgaatga tcccacgatt
    13741 aaaggcgggc gcttaatcgt tgaaaaagat gggattaccg tccttgccgc tatcgaaaag
    13801 ggaggattac tggaggttaa agagggggga ttagcgattg cggtagatca gaaagcaggc
    13861 ggtgctatta aagcaagcac gcgggtcatg gaggcattcg gaacaaaccg tctcggtcag
    13921 ttcgaaatca agaatggtat tgctaacaat atgctgttgg aaaacggcgg aagtttgcga
    13981 gttgaagaaa atgacttcgc ttataatact actgtagata gtggcggctt actggaggtt
    14041 atggatggcg ggactgtaac tggcgttgat aaaaaagcag gcggaaaatt aattgtctca
    14101 acgaatgcgc tggaagtgag tggtacaaac agtaaaggcc aatttagtat aaaagatggt
    14161 gtgtcaaaaa attatgaact ggatgatggt tccgggctca ttgttatgga ggacacgcag
    14221 gccattgata ctatccttga tgagcatgcc actatgcaat cgctgggaaa ggatactggt
    14281 acgaaagtgc aggcaaatgc ggtatatgat ctcggtcgat cagatcagaa tggaagtatc
    14341 acgtattcct caaaagccat ttctgaaaat atggttatca acaatggccg cgctaacgtc
    14401 tgggctggca caatggttaa cgtttcagtc agagggaatg atggcattct tgaggtcatg
    14461 aagccgcaaa taaattatgc acccgcaatg ttggtgggta aggtagtggt ttctgagggc
    14521 gcttctttta gaacgcatgg tgccgtggat accagcaaag cggacgtttc gctcgaaaat
    14581 agcgtatgga ccatcattgc cgatatcact acgacgaacc aaaacaccct cctcaactta
    14641 gccaaccttg cgatgtctga cgcaaatgtg attatgatgg atgagccagt gactcgttca
    14701 tcagtgacgg caagtgcgga aaatttcatt acgttgacca ccaataccct gtcgggaaac
    14761 ggcaattttt atatgcgtac cgatatggct aatcatcaga gcgatcagct caacgtcacc
    14821 ggtcaggcaa caggtgattt caaaatattc gtgacggaca ccggtgccag cccggcagca
    14881 ggagatagcc ttacactggt aacaacgggc ggcggtgatg ctgcatttac gttgggcaat
    14941 gccggaggcg ttgttgatat cggtacgtat gaatatacct tgctggataa tggcaaccat
    15001 agctggagtt tggcagagaa tcgcgcgcaa attacccctt caaccactga tgtgctgaat
    15061 atggcggcag cacaaccgct ggtatttgat gcagaactgg acaccgtgcg tgagcgtctt
    15121 ggtagcgtaa aaggcgttaa ttacgatacg gcgatgtgga gttcggcaat taacacccgc
    15181 aacaacgtga ccactgatgc gggcgctggc tttgagcaaa cattgaccgg gctgacgctc
    15241 ggtatcgata gccgtttctc ccgtgaagaa accagcacaa ttcgcggctt gttctttggt
    15301 tactctcatt ctgatattgg ttttgatcgc ggcggcaaag gcaatgtcga tagctatacc
    15361 ctgggggctt atgccggttg ggagcatcag aacggtgcct atgttgatgg agtggtgaaa
    15421 gttgaccgtt ttgccaacac catccattgc aagatgagta atggggcaac agcgtttggc
    15481 gattacaata gtaacggcgc gggtgctcat gtcgagagcg ggttccgttg ggttgacgga
    15541 ttgtggagtg ttagacccta tctggccttt accggcttta ccacagatgg tcaggactac
    15601 acgttatcaa acggcatgcg cgcggatgtg ggaaataccc ggatattacg cgctgaagcg
    15661 ggaacggcgg taagctatca catggacctg caaaacggta cgacgctgga accctgactg
    15721 aaagccgccg tgcgtcagga atacgccgat tctaaccagg tgaaagttaa tgacgatggc
    15781 aaatttaata atgatgtggc tggaacccgt ggcgtttatc aggctgggat aaggtcatcg
    15841 tttaccccga cgttaagcgg tcatttgtca gtcagctatg gcaatggcgc aggggtagaa
    15901 tcgccgtgga atactcaggc gggtgtggcc tggacgttct gataacaaaa aataaacagg
    15961 ctgtgatgtg tcacggtctg tttatcgaat taattgcaga tataaaaaaa accaaccgta
    16021 aaggttgatt ttttcttggg atttttggca cgagagtagt ttaattgagc gtgt
//
LOCUS       DAZFQD010000121        16071 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.121, whole genome shotgun sequence.
ACCESSION   DAZFQD010000121 DAZFQD010000000
VERSION     DAZFQD010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16071
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.121"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..4610
                     /locus_tag="ACHKTW_003891"
     CDS             <1..4610
                     /locus_tag="ACHKTW_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ECSE_1600 family autotransporter"
                     /protein_id="HFO6464823.1"
                     /translation="NATGVGTLNVQDQDSVITVRRLYNGYFGNGAVNISNNGLINNKE
                     YSLVGVEDGSHGVVNVTDKGHWSFLGTGEAFRYIYIGDAGDGELNVSREGKVDSGIIT
                     AGMKETGTGNITVKDKNSVITNLGTNLGYDGHGEMNISNEGLVVSNGGSSLGYGENGV
                     GNVSITTGGMWEVNKNVYTTIGVAGVGNLNISDGGKFVSQNITFLGDKASGIGTLNLM
                     DGTSSFDTVGINVGNFGSGIVNVSNGATLNSTGYGFIGGNASGKGIVNISTDSLWNLK
                     TSSTNAQLLQVGVLGTGELNITTGGIVKARDTQIALNDKSKGDVRVDGQNSLLETFNM
                     YVGTSGTGTLTLTNSGTLNVEGGEVYLGVFEPAVGTLNIGAAHGEAAADAGYITNATK
                     VEFGSGEGVFVFNHTNNSDAGYQVDMLITGDDKDGKVIHDAGHTVFNAGNTYSGKTLV
                     NDGLLTIASHTADGVTGMGSSEVTIASPGTLDILASTNSAGDYTLTNALKGDGLMRVQ
                     LSSSDKMFGFTHATGTEFAGVAQLKDSTFTLERDNTAALTHAMLQSDSENTTSVKVGE
                     QSIGGLAMNGGTLIFDTDIPAATLAEGYISVDTLVVGAGDYTWKGRNYQVNGTGDVLI
                     DVPKPWNDPMANNPLTTLNLLEHDDSHVGVQLVKAQTVIGSGGSLTLRDLQGDEVEAD
                     KTLHIAQNGTVVAEGDYGFRLTTAPGDGLYVNYGLKALNIHGGQKLTLAEHGGAYGAT
                     ADMSAKIGGEGDLAINTVRQVSLSNGQNDYQGATYVQMGTLRTDADGALGNTRELNIS
                     NAAIVDLNGSTQTVETFTGQMGSTVLFKEGALTVNKGGISQGELTGGGNLNVTGGTLA
                     IEGLNARYNALTSISPNAEVSLDNTQGLGRGNIANDGLLTLKNVTGELRNSISGKGIV
                     SATARTDVELDGDNSRFVGQFNIDTGSALSVNEQKNLGDASVINNGLLTISTERSWAM
                     THSISGSGDVTKLGTGILTLNNDSAAYQGTTDIVGGEIAFGSDSAINMASQHINIHNS
                     GVMSGNVTTAGDVNVMPGGTLRVAKTTIGGNLENGGTVQMNSEGGKPGNVLTVNGNYT
                     GNNGLMTFNATLGGDNSPTDKMNVKGDTQGNTRVRVDNIGGVGAQTVNGIELIEVGGN
                     SAGNFALTTGTVEAGAYVYTLAKGKGNDEKNWYLTSKWDGVTPPDTPDPINNPPVVDP
                     EGPSVYRPEAGSYISNIAAANSLFSHRLHDRLGEPQYIDSLHSQGSASSMWMRHVGGH
                     ERSRAGDGQLNTQANRYVLQLGGDLAQWSSNAQDRWHLGVMAGYANQHSNTQSNRVGY
                     KSDGRISGYSAGLYATWYQNDANKTGAYVDSWALYNWFDNSVSSDNRSADDYDSRGVT
                     ASVEGGYTFEAGTFSGSEGTLNTWYVQPQAQITWMGVKDSDHTRKDGTRIETEGDGNV
                     QTRLGVKTYLNSHHQRDDGKQREFQPYIEANWINNSKVYAVKMNGQTVGREGARNLGE
                     VRTGVEAKVNNNLSLWGNVGVQLGDKGYSDTQGMLGVKYSW"
     gene            4819..5085
                     /gene="hipB"
                     /locus_tag="ACHKTW_003892"
     CDS             4819..5085
                     /gene="hipB"
                     /locus_tag="ACHKTW_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HipB"
                     /protein_id="HFO6464824.1"
                     /translation="MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISN
                     FENNPDNTTLTTFFKILQSLELSMTLCDTKNASPESTEQQDLEW"
     gene            5085..6407
                     /gene="hipA"
                     /locus_tag="ACHKTW_003893"
     CDS             5085..6407
                     /gene="hipA"
                     /locus_tag="ACHKTW_003893"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system serine/threonine
                     protein kinase toxin HipA"
                     /protein_id="HFO6464825.1"
                     /translation="MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLS
                     LPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYHAKSRQPFDLLSEIGRDSVGAVT
                     LLPENETITRPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
                     LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVP
                     DAEIIKAGNVRALAVERFDRRWNAERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIA
                     RIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQAGGSYRLTPFYDII
                     SAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLRFPEVQMHEIL
                     SDFARMIPAALDNVKTSLPTDFPENVVTAVETNVLRLHGRLSREYGSK"
     gene            6720..7295
                     /gene="fimA"
                     /locus_tag="ACHKTW_003894"
     CDS             6720..7295
                     /gene="fimA"
                     /locus_tag="ACHKTW_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002916190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HFO6464826.1"
                     /translation="MSPLRYQKCVGMIVVSVLAMSSAAVSAAEGDESVTTTVNGGVIH
                     FKGEVVNAACAIDSESMNQTVELGQVRSSRLAKAGDLSSAVGFNIKLNDCDTNVSSNA
                     AVAFLGTTVTSNDDTLALQSSAAGSAQNVGIQILDRTGEVLILDGATFSAKTDLIDGT
                     NILPFQARYIALGQSVAGTANADATFKVQYL"
     gene            7656..8366
                     /locus_tag="ACHKTW_003895"
     CDS             7656..8366
                     /locus_tag="ACHKTW_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFO6464827.1"
                     /translation="MQTTRTPYSISFMATVLLLLLFACHSTVANAAVALGATRVIYPA
                     NQKQVLLPVTNNDPASVYLIQSWIENAGDQKDTQFVITPPLFSMQGKKENTLRIINAT
                     NHQLPGDRESLFWVNVKAIPAMEKDQKNENTLQLAIISRIKMFYRPTNLAMAPEEAPA
                     MLRFRRSGSKLTLINPTPYFITVTNMKAGNSNLPNTMVPPKGEVSVDITHAATGDISF
                     QTINDYGALTPRIKATMQ"
     gene            8408..11059
                     /locus_tag="ACHKTW_003896"
     CDS             8408..11059
                     /locus_tag="ACHKTW_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFO6464828.1"
                     /translation="MTAFRAAFKAYRMHQVLIMPRFARLTIALGLATAVFPVDAEYYF
                     NPRFLSNDLAESVDLSAFTKGREAPPGTYRVDIYLNDEFMTSRDITFIADDNNAELIP
                     CLSTDLLVSLGIKKIALLDNKEHSAEKHVPDNSACTPLQDRLADASTEFDVGQQHLSL
                     SVPQIYVGRMARGYVSPDLWEEGINAGLLNYSFNGNSINNRSNHNAGKSNYAYLNLQS
                     GINIGSWRLRDNSTWSYYSGSSNSSDSNKWQHINTSAERDIIPLRSRLTVGDSYTDGD
                     IFDSVNFRGLKINSTEAMLPDSQHGFAPVIHGIARGTAQVSVKQNGYDVYQTTVPPGP
                     FTIDDINSAANGGDLQVTIKEADGSIQTLYVPYSSVPVLQRAGYTRYALAMGEYRSGN
                     NLQSSPKFIQGSLMHGLEGNWTPYGGMQIAEDYQAFNLGIGKDLGLFGAFSFDITQAN
                     TTLADGTRHSGQSVKSVYSKSFYQTGTNIQVAGYRYSTQGFYNLSDSAYSRMSGYTVK
                     PPTGDTNEQTQFIDYFNLFYSKRGQEQISISQQLGNYGTTFFSASRQSYWNTSRSDQQ
                     ISFGLNVPFGDITTSLNYSYSNNIWQNDREHLLAFTLNVPFSHWMRTDSQSAFRNSNA
                     SYSMSNDLKGGMTNLSGVYGTLLPDNNLNYSVQVGNTHGGNTSSGTSGYSSLNYRGAY
                     GNTNVGYSRSGDSSQIYYGMSGGIIAHADGITFGQPLGDTMVLVKAPGADNVKIENQT
                     GIHTDWRGYAILPFATEYRENRVALNANSLADNVELDETVVTVIPTHGAIARATFNAQ
                     IGGKVLMTLKYGNKSVPFGAIVTHGENKNGSIVAENGQVYLTGLPQSGKLQVSWGKDK
                     NSNCIVEYKLPEVSPGTLLNQQTAICR"
     gene            11073..11603
                     /gene="ydeS"
                     /locus_tag="ACHKTW_003897"
     CDS             11073..11603
                     /gene="ydeS"
                     /locus_tag="ACHKTW_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO6464829.1"
                     /translation="MKYNNIIFLGLCLGLTTYSALSADSVIKISGRVLDYGCTVSSDS
                     LNFTVDLQKNSARQFPTTGSTSPAVPFQITLSECSKGTTGVRVAFNGIEDAENNTLLK
                     LDEGSNTASGLGIEILDANMRPVKLNDLHAGMQWIPLVPEQNNILPYSARLKSTQKSV
                     NPGLVRASATFTLEFQ"
     gene            11616..12119
                     /gene="ydeR"
                     /locus_tag="ACHKTW_003898"
     CDS             11616..12119
                     /gene="ydeR"
                     /locus_tag="ACHKTW_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO6464830.1"
                     /translation="MKRLHKRFLLATFCALFTATLQAADVTITVNGRVVAKPCTIQTK
                     EANVNLGDLYTRNLQQPGSASGWHNITLSLTDCPVETSAVTAIVTGSTDNTGYYKNEG
                     TAENIQIELRDDQDAALKNGDSKTVIVDEITRNAQFPLKARAITVNGNASQGTIEALI
                     NVIYTWQ"
     gene            12178..13092
                     /gene="ydeQ"
                     /locus_tag="ACHKTW_003899"
     CDS             12178..13092
                     /gene="ydeQ"
                     /locus_tag="ACHKTW_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO6464831.1"
                     /translation="MGKTISIKVLFGIYLLLMAGKVFAFSCNVDGGSSIGAGTTSVYV
                     NLDPVIQPGQNLVVDLSQHISCWNDYGGWYDTDHINLVQGSAFAGSLQSYKGSLYWNN
                     VTYPFPLTTNTNVLDIGDKTPMPLPLKLYITPVGAAGGVVIKAGEVIARIHMYKIATL
                     GSGNPRNFTWNIISNNNVVMPTGGCTVDSRNVTVDLPDFPGSAEIPLGVYCSSEQKLS
                     FYLSGAITDSSRQVFANTAPDATKASGVGVTLMRNGKILATGENVSLGTVNKSKVPLG
                     LSATYGQTGNKVSAGTVQSVIGVTFIYE"
     gene            13426..15693
                     /gene="ydeP"
                     /locus_tag="ACHKTW_003900"
     CDS             13426..15693
                     /gene="ydeP"
                     /locus_tag="ACHKTW_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HFO6464832.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRQDVIAMFDMNKP
                     EGFDCPGCAWPDPKHSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLSWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAAERTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFTPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGITQATLPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSNTTIYGMDDRYRGVFGQRDVVFMSAKQAKICRV
                     KNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHDPLSG
                     IPGYKSIPVELEPSN"
     gene            15941..>16071
                     /gene="safA"
                     /locus_tag="ACHKTW_003901"
     CDS             15941..>16071
                     /gene="safA"
                     /locus_tag="ACHKTW_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HFO6464833.1"
                     /translation="MHATTVKNKITQRDNYKEIMSVIVVVLLLTLMLIAIFSAIDQLG
                     "
BASE COUNT         4449 a         3514 c         4022 g         4086 t
ORIGIN      
        1 gcaatgccac cggtgtcgga accctgaatg tgcaggatca agactctgtc atcacggtac
       61 gcagactcta taatggatat ttcggtaatg gcgcagtcaa tatttccaat aatggactaa
      121 ttaataacaa agaatattca ttggtgggcg ttgaagacgg ttcccacggt gtcgtcaacg
      181 tgaccgataa agggcattgg agtttcctcg gaacaggcga agctttccgc tatatctata
      241 tcggtgatgc tggtgacggt gaacttaatg tctcgcgtga aggtaaagta gattcgggaa
      301 ttatcactgc ggggatgaaa gaaacaggca caggcaacat tactgttaag gataagaact
      361 ccgttatcac taatctcgga actaatcttg gttatgacgg ccacggcgaa atgaatatca
      421 gtaatgaggg gcttgttgtc agcaacggag gaagctcact cggttatgga gaaaacggcg
      481 tcgggaatgt cagcatcacc acggggggga tgtgggaggt caataagaat gtctatacca
      541 ccattggtgt tgcgggcgtc ggaaacctca atattagcga tggcggtaag tttgtatcgc
      601 aaaatattac ttttttgggc gataaagcaa gcggtatcgg cacactgaac ctgatggatg
      661 ggacatcatc gttcgatact gtgggtatca atgtcggtaa ttttggtagc ggtatcgtaa
      721 atgtcagtaa tggtgccacc cttaattcaa cgggctatgg atttatcgga ggaaatgcct
      781 ccggtaaagg gatagttaat atttcaacgg atagtctctg gaatttaaag acatcatcta
      841 ctaacgccca attgctacag gtcggtgtat taggcacggg tgaactgaat attaccaccg
      901 gaggtatagt taaagcgcgt gatacacaga tagctctcaa tgacaaaagt aagggcgacg
      961 tgagggtgga tgggcagaac tctcttcttg aaacattcaa tatgtacgta gggacatctg
     1021 gtacgggtac gttaaccctg acgaatagcg gcacgctgaa tgtcgaaggt ggagaagttt
     1081 acttaggtgt ttttgaacct gctgtaggaa cgctaaacat tggtgcagct cacggtgagg
     1141 cggcggcaga tgccgggtat ataaccaacg cgacgaaagt agagtttggt tctggcgaag
     1201 gtgtttttgt ctttaatcat actaataaca gtgatgccgg ctaccaggtc gatatgctga
     1261 ttacgggtga cgataaagac ggaaaagtga tccatgatgc aggccatacg gtgttcaatg
     1321 cagggaatac ttatagcggt aaaacgctgg tcaatgacgg cctcctgacc attgcgtctc
     1381 atacggcaga tggggtaacg ggcatgggtt cgagtgaagt caccattgca agccccggta
     1441 cgctcgacat tctcgcatca acgaacagtg caggagatta cacgctgacc aatgcgctca
     1501 aaggcgatgg tttgatgcga gtgcagctgt catcctccga caagatgttt ggctttaccc
     1561 atgcaacagg gactgaattc gccggtgttg cccaactgaa agacagtacc ttcactctgg
     1621 aacgcgacaa caccgctgcg cttactcacg cgatgttgca gtctgacagt gaaaatacca
     1681 catcggtaaa agtgggagag caatccattg gtggactggc catgaatggc ggtacgctca
     1741 ttttcgatac ggatattcct gctgcgacgc ttgcagaggg atatatcagc gtcgatacgc
     1801 tggttgtcgg cgcgggtgac tacacctgga aaggccgtaa ctatcaggta aacgggacgg
     1861 gcgacgtgct tatcgacgtg cctaaaccgt ggaatgatcc catggcgaat aaccctctga
     1921 cgacgctcaa tttgctggaa cacgacgata gccatgtcgg cgttcaactg gtgaaggcgc
     1981 aaacggttat tgggtcgggt ggctcattaa cgttacgtga tttacagggc gacgaggtgg
     2041 aagcggacaa aacgttacac attgcgcaaa acggaacggt ggtcgccgag ggtgattatg
     2101 gattccgcct cacgaccgca ccaggtgatg gtttgtacgt taactatggg ctgaaagcgc
     2161 tgaacatcca tggtgggcaa aagctgacgt tagccgaaca tggcggagcc tatggcgcaa
     2221 cggccgatat gtcggcaaaa atcggtggtg aaggggatct ggcaatcaat acggtgcgac
     2281 aggtttcgct ttccaacggt cagaacgact atcagggggc aacctacgtt cagatgggga
     2341 cattacgtac cgatgcggat ggtgcacttg gcaacacccg ggaactgaac atcagcaacg
     2401 cggccatcgt cgatcttaat ggatctacgc agacggtaga gacattcacc gggcagatgg
     2461 gttcgactgt tttgttcaaa gagggggcgc tgacggtaaa taaaggtggg atcagtcagg
     2521 gtgaactgac aggtggcgga aacctgaatg ttacaggggg aacgctggct atcgaggggc
     2581 ttaatgcacg ctacaatgcg ttaaccagca ttagcccaaa tgcggaagtc agcctcgata
     2641 atactcaggg gttaggcaga ggaaatattg ccaatgacgg tctgttaacg ctaaaaaacg
     2701 tgactggcga actgcgtaat agcataagcg ggaagggtat cgtgagcgca accgccagga
     2761 cagatgtaga gctggatggc gataatagcc gctttgtggg gcaattcaac attgatacag
     2821 gcagcgcgct cagcgtcaac gagcagaaaa acctgggtga tgcttccgtt atcaataatg
     2881 gcctgctcac catctccact gagcgtagct gggcgatgac gcacagtatc agcggtagcg
     2941 gtgatgtgac aaaactgggt accgggatcc tgactcttaa caacgattcc gcggcgtatc
     3001 agggtacgac ggatatcgtg gggggggaaa ttgctttcgg ttccgactct gccattaata
     3061 tggcaagtca acacattaat atccataaca gcggtgtgat gtcgggaaat gtcaccactg
     3121 caggtgatgt gaacgttatg cctgggggga cactgcgtgt cgctaaaacc acaatcggcg
     3181 gcaacctgga gaatggcggc acggttcaaa tgaatagcga aggggggaaa ccggggaatg
     3241 tactgaccgt taacggcaac tataccggaa acaatggcct gatgacgttc aacgcgacgc
     3301 tgggcggcga taattcgccc accgataaaa tgaacgtgaa aggcgatacc caagggaaca
     3361 ctcgcgttcg ggttgataac attggcggcg tcggtgcaca aacggtcaac ggtattgaac
     3421 tcattgaggt tggcggtaat tctgcaggta acttcgcgct gaccaccgga actgtcgaag
     3481 ctggggctta cgtctacacg ctggctaaag ggaaggggaa tgacgagaaa aactggtatc
     3541 tgaccagtaa atgggacggc gtaacgccac cggatacgcc cgaccccatc aataatcccc
     3601 ctgttgtgga tccggaaggc ccatcagttt atcgcccgga ggccggaagc tatatcagca
     3661 acattgccgc agccaactcg ctgtttagcc atcgtttaca cgaccgtctg ggtgagccgc
     3721 agtatataga ttcactgcat tctcaggggt cggcaagcag tatgtggatg cgtcatgtcg
     3781 gagggcacga acgttcaagg gccggtgacg gtcagctaaa tactcaggct aaccgctatg
     3841 tattgcagct aggcggcgat ttggcgcagt ggagtagcaa cgcgcaggat cgctggcatc
     3901 ttggcgtgat ggcaggttac gctaatcagc acagtaatac tcagagtaat cgtgtgggtt
     3961 ataaatcgga tgggcgcatc agcggttaca gcgctgggct gtacgcgacc tggtatcaga
     4021 acgatgcgaa taagaccggc gcttatgttg acagctgggc gctgtataac tggtttgata
     4081 acagcgtcag ttccgataac cgttctgctg acgactatga ttctcgcggt gtgacggcct
     4141 ctgttgaggg tgggtatacc tttgaagcgg gaacatttag cggcagcgaa gggacgctga
     4201 atacctggta cgtccagcca caggcgcaaa tcacctggat gggtgtgaaa gattccgacc
     4261 atacccggaa agacggaacg cgcattgaaa cggaaggcga cggaaatgtg caaacgcgac
     4321 ttggggtgaa aacctacctg aacagccatc accagcgtga cgatggtaaa cagcgtgagt
     4381 tccagcctta cattgaagcg aattggatca acaacagcaa agtctacgcc gtgaagatga
     4441 atggtcaaac cgtaggccgt gaaggtgcgc gtaatctcgg tgaagtacgt accggggttg
     4501 aggcgaaagt aaataacaac cttagcctgt gggggaatgt cggtgtgcaa ctaggtgata
     4561 aaggctatag cgatactcag ggcatgctgg gagtgaaata tagctggtaa accgtataag
     4621 ccgcatgtcg agatggcatg cggcttaata ttgccgactt caaaggcttt aaagcctccc
     4681 ttttatccgc gatcgcggat atcgcagcgt ttatcccgta cagcggataa gatgtgtttc
     4741 cagattgact ttatcctcac taaaggataa aactgataat atccccttaa gcggataaat
     4801 ttgctgtgga cgtatgacat gatgagcttt cagaagatct atagcccaac gcaattagcg
     4861 aatgcaatga aactggttcg ccagcaaaat ggctggacgc agagcgagct ggcgaaaaaa
     4921 attggcatta agcaggcgac aatttccaat ttcgaaaaca accctgacaa taccacgctc
     4981 acgacatttt ttaagatttt acagtcgctt gaactctcaa tgacactatg cgacacgaaa
     5041 aatgcctcgc cagaatcaac agaacagcaa gatctggagt ggtaatgcct aaacttgtca
     5101 cttggatgaa caaccagcgg gtaggcgagt taacgaagtt agccaacggc gcgcacacct
     5161 ttaagtatgc accggagtgg ttagccagcc gttatgccag accgttgtca ctttcgctgc
     5221 cattgcagag ggggaatatc acctcagatg ccgtatttaa cttcttcgat aacctgttac
     5281 ccgatagccc gattgtacgt gaccggatcg ttaaacgtta tcatgctaaa tccagacaac
     5341 cgtttgattt attgtcagaa atagggcgag acagcgttgg tgccgtaacg ttattacccg
     5401 aaaatgaaac cataacgcgc ccgataatgg catgggaaaa gcttactgaa gccaggcttg
     5461 aagaagtatt aacggcttat aaagcagata tccctctggg catgattaga gaagaaaatg
     5521 actttcgcat ctcggttgct ggcgcgcagg agaagacagc gctgcttaga ataggcaatg
     5581 actggtgcat tccgaaagga ataacgccga cgacgcacat cattaaatta ccgattggcg
     5641 aaatcaggca gcccaatgcg acgctcgatc tcagccaaag cgttgataat gagtattact
     5701 gtctgctgct ggcgaaagaa cttgggttga atgttccgga cgcagaaatc attaaagcgg
     5761 gaaatgtgcg cgcgttagcg gtcgaacgtt ttgacaggcg ttggaatgct gagcgaacgg
     5821 ttttacttcg cttgccacag gaggatatgt gtcagacatt cggtttacct tcatcggtga
     5881 aatatgaatc agatggaggc ccaggcatcg cgcggatcat ggcttttttg atggggtcca
     5941 gcgaggcgct gaaagatcgc tatgatttta tgaaattcca ggtcttccag tggttgattg
     6001 gcgcaacgga cggtcatgca aaaaacttct ccgtatttat tcaggctggc ggcagttatc
     6061 gactcacgcc attttacgac atcatttcag catttccggt ccttggcggt acgggaatac
     6121 acatcagcga tctcaaactg gcaatggggc ttaacgcatc caaaggcaaa aaaacggcaa
     6181 tcgataaaat ttatccgcga cattttttgg cgacagcaaa ggtgctgaga ttcccggaag
     6241 tgcagatgca tgaaatccta agtgactttg ccagaatgat tccggcagca ctggataacg
     6301 tgaagacttc attaccgaca gattttccgg agaacgtggt gacggcagtt gaaaccaatg
     6361 tgttgaggtt gcatggacgg ttaagccgag aatacggtag taagtgagat atggggtttg
     6421 gtcgttgtta atgaacaatc atgaccgcca acgactggat tcttccttgg tttatactgg
     6481 tgcgcataat gtatattatg ttaaatcatg tctgagtgat gcgaagtgct tttctggtag
     6541 tcgttattcg ttcagggttc aagaacttaa gcctctcctc tttcataatt ggcctgtgca
     6601 tggagaaata gcctggttat tccctgcccc atgctaagag cgcagtgtct gtcgcataga
     6661 cactgcatac gaaaacacca gagggtgctg ttacttattt aaggacggat caagcgggaa
     6721 tgagtccact ccggtatcag aaatgtgttg gtatgattgt cgtttctgtg ttggcgatgt
     6781 cgtctgctgc ggtaagcgca gccgagggtg atgaatcagt aacgaccact gttaatggcg
     6841 gtgttattca ttttaaaggt gaagtggtaa atgccgcttg tgcaattgat tcagaatcaa
     6901 tgaaccaaac ggttgagctg ggtcaggttc gttcttctcg cctggctaaa gcgggtgacc
     6961 tcagctccgc cgttggcttc aatatcaagc tgaatgattg tgataccaat gtttccagta
     7021 atgcagctgt tgcattcctg ggtactactg tcaccagtaa tgacgatacg ttagcgctgc
     7081 agagttcagc ggcaggctct gcccaaaatg tcggtattca aattttggac cgtacgggtg
     7141 aggtattaat acttgatggg gccactttta gtgctaaaac cgacttgatt gatggcacga
     7201 atatactgcc attccaggct cgttatattg ctctcgggca gtccgtagct ggtactgcaa
     7261 acgctgatgc gaccttcaaa gttcaatatc tataattcaa tcttaaggtg gctatacgtc
     7321 atctgaaatt gaaacagggt gtgtgtggca tggctacgca cacccgagca atttaccaat
     7381 attcatctca tttttaatga gaaatggttt gtatttggag cgctatgaaa aatcacataa
     7441 tatctaaact atcaataaac aacagtagtt aaacttactg ttatttaaaa tatttacttt
     7501 gtagcaaggt tttcctatta aaagagaacc aacatttatt acaaacgcat taagtctgga
     7561 aacccggcga ctcgccaatg tattagcaac attaatcgat tgtctgatgt aacggttttc
     7621 ccgcttatcc tcttaacaca tggagaatgc atcagatgca aactacgcgt accccctact
     7681 ctatttcctt tatggcgaca gtgttacttt tattgctctt tgcttgccat agcaccgttg
     7741 ccaacgcagc tgtcgcactg ggtgcgacgc gggtaattta tccggcaaac cagaaacagg
     7801 ttttattgcc tgtcacgaat aatgatcctg ccagtgtcta tctcattcag tcatggatag
     7861 aaaatgcagg tgaccaaaaa gacacccagt ttgtcattac cccaccgctt ttttccatgc
     7921 aaggtaaaaa agagaatacg ctgcggataa tcaacgctac taatcatcag ttaccaggcg
     7981 accgtgaaag cctgttctgg gtgaatgtta aagctattcc tgctatggaa aaagaccaga
     8041 agaatgagaa tacattacag ctggcaatta ttagccgcat aaaaatgttt tatcgcccca
     8101 cgaacctggc aatggcacct gaagaagcgc ctgcaatgct tcggtttcgt cgcagcggta
     8161 gcaaactcac gcttattaat ccaacgccct atttcattac cgtaacaaat atgaaagcag
     8221 gaaatagcaa tctcccaaac accatggtgc cgccaaaagg tgaagtatcg gtggatatca
     8281 cgcatgctgc aaccggggat atcagttttc agacgatcaa tgattacggt gcattaactc
     8341 cgcgtattaa ggcaaccatg cagtaagcca gtgacctaat taataataaa ctcaatcgga
     8401 attaataatg acagcctttc gtgccgcatt taaggcatat cgtatgcatc aggtgttaat
     8461 aatgcctcgc tttgcacggc ttaccattgc ccttggctta gcaactgcgg tatttcccgt
     8521 tgacgcagag tattatttta atccacggtt tttaagcaat gatcttgctg aatctgtcga
     8581 tttatccgcc tttactaagg gacgcgaggc accacctggt acatatcggg tcgacattta
     8641 tttgaacgac gaatttatga ctagtcggga tattactttt attgcagatg ataataacgc
     8701 agaactcatc ccatgcctga gtacagatct tcttgtcagt cttggtataa aaaaaatagc
     8761 gttattagac aataaagaac attcggcaga aaaacatgtg ccggacaaca gcgcatgcac
     8821 accacttcag gatcgtttgg ccgacgcatc aactgagttt gatgttggtc agcaacattt
     8881 gtcactctcc gttccgcaaa tttatgttgg cagaatggct cgcggctatg tttctcctga
     8941 tctgtgggaa gaaggaataa atgctgggct tctaaactat agttttaatg gtaattctat
     9001 taataatcgt agtaaccata atgcaggaaa atccaactat gcatatttga atttacagag
     9061 tggcatcaac attggtagtt ggcgactgcg cgataactca acgtggagtt attacagtgg
     9121 gagtagcaat tcatctgaca gcaataaatg gcagcatatc aatacgtcgg ctgaacgtga
     9181 cattattccc ttacgctcac ggttaacggt aggtgatagt tataccgatg gcgatatttt
     9241 tgatagtgtg aactttcgtg gcctcaaaat aaattcaaca gaagcgatgt tgcccgatag
     9301 ccaacatggt ttcgctccgg tgattcatgg tattgcccgt ggaaccgcac aagtgagtgt
     9361 aaaacagaac ggatacgatg tttatcagac tactgttcca cccggccctt ttactattga
     9421 tgatatcaac tctgcggcca atggtggtga tctgcaagta accataaaag aggcagacgg
     9481 cagtattcag acattatatg ttccttattc gtctgttccg gttctccaac gtgctggata
     9541 tacgcgttat gcgcttgcca tgggggaata tcgtagtgga aataacctgc aaagctctcc
     9601 caagttcata caaggtagct tgatgcatgg tctggaagga aactggacac cttatggcgg
     9661 aatgcaaatt gcagaagatt atcaggcctt caaccttggt attgggaaag atttaggact
     9721 ttttggtgcc ttttctttcg atatcacgca ggccaatacg acacttgcag atggcacccg
     9781 tcacagtggg caatcggtta aatccgtcta cagcaaatcc ttctaccaga caggaaccaa
     9841 tatccaggtc gcaggatatc gctattctac gcaaggtttt tataacttat ccgacagtgc
     9901 ctacagtcga atgagtggtt acaccgtcaa gcctcctacc ggagacacca atgagcagac
     9961 acaatttatt gattatttta atctgttcta cagtaagcgt ggtcaggaac aaataagcat
    10021 ctctcagcag cttggaaatt acggtacgac atttttcagt gccagtcgcc aaagttactg
    10081 gaacacgtca cgcagcgacc agcaaatatc atttggatta aatgtgccgt ttggtgatat
    10141 tacgacttcg ctgaattaca gctattccaa taatatatgg caaaacgatc gggaacattt
    10201 actcgctttt acgcttaatg ttcccttcag tcattggatg cgtacagaca gtcagtcggc
    10261 atttcgtaat tcaaacgcca gttacagtat gtcaaacgat ttgaaaggcg gcatgaccaa
    10321 tctatcgggg gtttatggca ctctgctgcc ggataataac ctgaattata gcgttcaggt
    10381 cggtaacacc cacggaggta atacatcgtc tggcaccagt ggttacagtt ctcttaatta
    10441 tcgtggagct tatggtaata ctaatgtcgg ttacagtcgg agtggtgaca gcagccagat
    10501 ttattacgga atgagtggtg ggattattgc tcatgctgat ggcatcacct ttggacagcc
    10561 gctgggcgac acaatggttc tggttaaggc tcctggtgct gataatgtca aaatagagaa
    10621 ccagaccgga attcataccg actggcgtgg ctatgccata ttaccatttg cgacagaata
    10681 tagagaaaac cgtgttgctc ttaacgcgaa ttcccttgca gataatgttg aactggatga
    10741 aaccgtggtc actgtcatcc caactcacgg tgctattgcc agagcaacat ttaatgcaca
    10801 aatcggcggg aaagtattaa tgacgttgaa gtacggtaat aagagcgttc cattcggtgc
    10861 aattgtcaca cacggagaga ataaaaatgg cagcattgtc gcagaaaacg gtcaggttta
    10921 tctgactgga cttccacagt cagggaaatt acaggtttca tggggcaaag ataaaaactc
    10981 aaactgtatt gtcgagtaca agcttcctga agtttctcct ggtaccttac tgaaccagca
    11041 gacagcaatc tgtcgctaag gaacaataga ttatgaaata caataacatt attttcctcg
    11101 gtttatgtct ggggttaacc acctattctg ctttatccgc agatagcgtt attaaaatta
    11161 gcgggcgcgt cctcgattat ggctgcacag tctcatcgga ttcgcttaat tttaccgtag
    11221 atctccaaaa aaacagtgcc agacaatttc caacgaccgg tagcacaagt ccagccgtcc
    11281 cttttcagat tacgttaagt gaatgcagca aagggacaac gggggttcgg gttgcattta
    11341 acggtattga ggatgcagaa aataatactt tgttgaaact ggatgaagga agcaatacgg
    11401 cctccggttt gggtatagaa atattggacg caaatatgcg tccggtgaaa ctgaatgacc
    11461 ttcatgccgg gatgcagtgg atcccactgg taccagaaca gaacaatatt ttgccttact
    11521 ccgctcgtct gaagtcaact cagaagtccg tcaatccggg actggtgagg gcttcggcaa
    11581 cctttaccct tgaatttcaa taatcagagt cagatatgaa aagacttcac aagaggttcc
    11641 tgttagctac gttttgcgcg ttattcacag caactctcca ggccgccgat gtcactatca
    11701 ctgttaatgg tcgggtagtc gctaaaccct gcactattca aaccaaagaa gctaacgtta
    11761 atctcgggga tctttatacg cgcaatctgc aacaacctgg ttctgcatct ggctggcaca
    11821 atattacttt gtcattaacc gattgtccgg ttgaaacaag tgcagtgacg gcaatcgtga
    11881 caggttcaac tgacaatacg ggttattaca aaaatgaagg tactgccgaa aatattcaga
    11941 tagagctgag ggatgaccag gatgctgcgt taaaaaatgg cgatagcaaa acggttattg
    12001 ttgatgagat cactcgtaat gcacagtttc cacttaaggc aagagctatc acggtgaatg
    12061 gaaacgcaag ccagggaacg atcgaggcgc taatcaatgt gatctacacc tggcaataac
    12121 gtcgattcga ttttcttagc cttcttttta aatcaatttt taaccttagg atatatcatg
    12181 ggtaaaacaa tcagtataaa ggtcctgttc ggtatttatc ttctattaat ggcaggcaaa
    12241 gtttttgcct tcagttgtaa tgtcgatggc gggtcaagta ttggagcagg tacaacctct
    12301 gtttatgtta atctcgaccc tgtaatacag ccgggccaga atctggttgt agacttgtct
    12361 cagcatataa gttgctggaa tgattacggc ggctggtacg acactgatca tataaacctg
    12421 gtacaaggct cagctttcgc aggctcccta cagtcatata aaggatcgct gtactggaat
    12481 aatgtgacct accccttccc gttgacgaca aataccaacg tattggatat tggtgataag
    12541 acaccgatgc cattaccgtt aaaactctat attaccccag ttggggcggc cggtggagtt
    12601 gtcataaaag cgggggaagt cattgcccgt attcatatgt ataaaattgc aacattagga
    12661 agcggaaatc cgcgtaattt tacgtggaat attatttcaa ataacaatgt tgttatgcca
    12721 acaggcggct gcaccgttga tagtcgtaat gtcaccgtgg atctcccgga ctttcctgga
    12781 agcgcagaaa taccccttgg cgtatattgc tcaagcgaac aaaagctctc attttatctt
    12841 tcgggtgcca tcactgacag tagccgacaa gtgtttgcta acacagcacc tgatgcaaca
    12901 aaagcatcgg gagtgggtgt cactctaatg cgtaatggaa aaatactggc tacgggagaa
    12961 aatgtctccc tggggacagt aaataaaagc aaagtacctc tgggtttaag cgcaacctat
    13021 ggacaaacag gaaacaaggt ttcagccgga accgtccagt cagtcattgg tgtcactttt
    13081 atctatgaat aattgtgatt ttgaccttag tctatgcgct ccattcacga aaattgcgcg
    13141 gataattatt ttgtgaaggc tattagccta cacctgtaag aaaatccgcg cacaattgaa
    13201 gccagagtct tattttttat attgaaaaat atgttaattt aaacactatg agagatgatg
    13261 cttaacttat cgtatttaat ctattgttta acgataatag attaaaaata gaaagagagt
    13321 agtcccctac tcttatatat ccatgttggc gataatccct ttgtatgtac tgtgcatcat
    13381 cgctattaca aatcctaata attcatttcc acacaggata agtagatgaa gaaaaaaatt
    13441 gaatcctacc agggtgctgc aggtggttgg ggtgctgtca aatccgtagc gaatgcagta
    13501 cgtaagcaga tggatatacg ccaggatgtt attgccatgt ttgacatgaa taagccagag
    13561 ggctttgact gtccgggttg tgcatggcca gatcctaagc acagtgcgtc attcgacatt
    13621 tgtgaaaacg gcgcaaaagc aatcgcctgg gaagtcacgg ataagcaggt aaatgcctct
    13681 ttctttgctg agaatacggt tcaatcatta ctttcctggg gagatcacga gcttgaggct
    13741 gcgggacgtc tcactcagcc tttgaaatat gatgccgtca gcgactgtta caagccatta
    13801 agctggcaac aagctttcga cgaaattggc gcacgccttc aaagctatag tgatcccaat
    13861 caggttgaat tctatacttc gggccgcact tccaatgaag ctgcctttct ttatcagctt
    13921 tttgcccgtg aatacgggag caataacttt cccgactgct ccaacatgtg ccatgaaccg
    13981 acaagcgtgg gtttggcagc gagtatcggt gtaggtaaag ggaccgtgtt gctggaagac
    14041 tttgagaagt gcgatttagt catttgcatt gggcataacc ctggtacaaa ccaccctcgc
    14101 atgctgactt cgttgcgcgc tttagtgaaa cggggagcga aaatgatcgc catcaatcct
    14161 ctacaggaac gtggcctgga gcgatttacc gcaccgcaaa acccgtttga aatgctgacg
    14221 aactctgaga ctcagttggc cagtgcctac tacaacgtgc gcattggtgg cgatatggcg
    14281 ttgctcaagg ggatgatgcg cctgttaatt gagcgcgatg atgctgcaag tgccgcaggt
    14341 cggccctcat tgcttgatga cgaatttatt caaacgcata ccgtcggctt tgacgagcta
    14401 cgccgtgacg ttctcaattc cgagtggaaa gatatcgaac gtatttctgg actaagtcag
    14461 acacaaatcg ccgaactggc tgacgcatat gccgctgccg aacgcaccat tatctgttac
    14521 ggaatgggga tcactcagca cgaacatggt acccagaacg tacagcaact ggtcaatttg
    14581 ctgttgatga aaggtaacat tggcaagcct ggtgcgggta tctgcccact acgtggtcac
    14641 tctaatgtac agggcgaccg aaccgtcggt atcaccgaga aaccgtctgc agagtttctg
    14701 gctcgcctgg gtgagcgcta tggcttcacc ccacctcatg cacctggaca tgctgcaatt
    14761 gccagcatgc aagcaatatg tacggggcag gctcgagcat tgatctgcat ggggggcaac
    14821 tttgcactgg caatgccaga tcgggaagcg agcgctgtac cgttaacgca attagatttg
    14881 gcggtacacg tagccactaa gcttaaccgc tctcatctgc tgaccgcacg gcatagctat
    14941 atcctgccgg ttctggggcg tagcgagatt gatatgcaaa aaagcggtgc gcaggcggta
    15001 accgttgagg attcaatgtc gatgattcat gcctcgcgtg gcgtgttaaa acccgccggt
    15061 gtaatgctga aatcagagtg tgctgtggtc gcgggaatca cccaggcaac actaccccag
    15121 agcgtggtag cctgggagta tctggtggaa gattatgatc gcattcgcaa tgacattgaa
    15181 gctgtgctgc cagagttcgc cgactataac cagcgcatcc gtcatcccgg tggttttcac
    15241 ctgataaatg cagctgctga aaggcgctgg atgacgccgt caggtaaggc taatttcatt
    15301 accagcaaag ggctgttaga agatccctct tcagcgttta acagtaagct ggtcatggcg
    15361 acagtacgca gcaacacgac gatttatggt atggatgatc gctatcgagg ggtattcggt
    15421 caacgagatg tggtctttat gagtgctaaa caagctaaaa tttgccgtgt aaaaaacggc
    15481 gaaagagtta atcttattgc gcttacgcca gacggtaagc gcagctcacg ccgcatggat
    15541 agattaaaag tggtcattta ccctatggct gaccgctcac tggtgaccta ttttccagaa
    15601 tcgaatcaca tgctaactct tgataaccac gatccattaa gtggcattcc tggttataaa
    15661 agtattccgg ttgaattaga accatcaaat taattcgcct cctcatttct tctgctgtca
    15721 tccgcacagc agaagaattt ctcattgact attatcgcgc aatttgctca cattgattaa
    15781 attaaaccac acactataag atataaacct ctgcctacag ctgtaagaaa ctccgctcag
    15841 tactgaagtg ccagtcctat ttcctctttt ctccagcctg ttatattaag catactgatt
    15901 aacgattttt aacgttatcc gctaaataaa catatttgaa atgcatgcga ccacagtgaa
    15961 aaacaaaatc acgcaaagag acaactataa agaaatcatg tctgtaattg tggttgtctt
    16021 attactgacc cttatgttga tagccatttt ttcggcaatt gatcagctgg g
//
LOCUS       DAZFQD010000122        15687 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.122, whole genome shotgun sequence.
ACCESSION   DAZFQD010000122 DAZFQD010000000
VERSION     DAZFQD010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15687
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.122"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(89..1447)
                     /gene="plaP"
                     /locus_tag="ACHKTW_003902"
     CDS             complement(89..1447)
                     /gene="plaP"
                     /locus_tag="ACHKTW_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HFO6464834.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANFNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWLNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(1437..1499)
                     /gene="yoeI"
                     /locus_tag="ACHKTW_003903"
     CDS             complement(1437..1499)
                     /gene="yoeI"
                     /locus_tag="ACHKTW_003903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HFO6464835.1"
                     /translation="MGQFFAYATVITVKENDHVA"
     gene            complement(1714..2643)
                     /gene="yeeY"
                     /locus_tag="ACHKTW_003904"
     CDS             complement(1714..2643)
                     /gene="yeeY"
                     /locus_tag="ACHKTW_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFO6464836.1"
                     /translation="MKPLLDVLMILDALEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLALEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAI"
     gene            complement(2689..3513)
                     /gene="yeeZ"
                     /locus_tag="ACHKTW_003905"
     CDS             complement(2689..3513)
                     /gene="yeeZ"
                     /locus_tag="ACHKTW_003905"
                     /EC_number="1.1.1.290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO6464837.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLEPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            complement(3596..3850)
                     /gene="yoeB"
                     /locus_tag="ACHKTW_003906"
     CDS             complement(3596..3850)
                     /gene="yoeB"
                     /locus_tag="ACHKTW_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HFO6464838.1"
                     /translation="MKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGK
                     PEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAACRYHY"
     gene            complement(3847..4098)
                     /gene="yefM"
                     /locus_tag="ACHKTW_003907"
     CDS             complement(3847..4098)
                     /gene="yefM"
                     /locus_tag="ACHKTW_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HFO6464839.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDIIE"
     gene            4381..4431
                     /gene="hisL"
                     /locus_tag="ACHKTW_003908"
     CDS             4381..4431
                     /gene="hisL"
                     /locus_tag="ACHKTW_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HFO6464840.1"
                     /translation="MTRVQFKHHHHHHHPD"
     misc_feature    4407..4530
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            4577..5476
                     /gene="hisG"
                     /locus_tag="ACHKTW_003909"
     CDS             4577..5476
                     /gene="hisG"
                     /locus_tag="ACHKTW_003909"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HFO6464841.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPGLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            5482..6786
                     /gene="hisD"
                     /locus_tag="ACHKTW_003910"
     CDS             5482..6786
                     /gene="hisD"
                     /locus_tag="ACHKTW_003910"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HFO6464842.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     TRGDEALREYSAKFDKTTVTALKVSAEEIAAASERLSDELKQAMAVAVKNIETFHTAQ
                     KLPPVDVETQPGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCGVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MAHQVAEAVERQLAELPRAETARQALSASRQIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARELVDSITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNALKEQA"
     gene            6783..7853
                     /gene="hisC"
                     /locus_tag="ACHKTW_003911"
     CDS             6783..7853
                     /gene="hisC"
                     /locus_tag="ACHKTW_003911"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HFO6464843.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FQLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTLENWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIAPYPLSTPVADIAAQALSPQGIVAMRERVAQIIAER
                     EYLIAALKEIPCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPFLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            7853..8920
                     /gene="hisB"
                     /locus_tag="ACHKTW_003912"
     CDS             7853..8920
                     /gene="hisB"
                     /locus_tag="ACHKTW_003912"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HFO6464844.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELMKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVERYLAEQAMDRANSYVIGDRATDIQLAENMGITGLRYDRETLNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            8920..9510
                     /gene="hisH"
                     /locus_tag="ACHKTW_003913"
     CDS             8920..9510
                     /gene="hisH"
                     /locus_tag="ACHKTW_003913"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HFO6464845.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDVVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVHKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            9510..10247
                     /gene="hisA"
                     /locus_tag="ACHKTW_003914"
     CDS             9510..10247
                     /gene="hisA"
                     /locus_tag="ACHKTW_003914"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HFO6464846.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPEMVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDIDDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            10229..11005
                     /gene="hisF"
                     /locus_tag="ACHKTW_003915"
     CDS             10229..11005
                     /gene="hisF"
                     /locus_tag="ACHKTW_003915"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HFO6464847.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLDDAAKILSFGADKISI
                     NSPALADPTLITHLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETL
                     DWVQEVQKRGAGEIVLNMMNQDGVRNGYDLEQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            10999..11610
                     /gene="hisIE"
                     /locus_tag="ACHKTW_003916"
     CDS             10999..11610
                     /gene="hisIE"
                     /locus_tag="ACHKTW_003916"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HFO6464848.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVSIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGDTAHQWLFLYQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLRKRHQ"
     gene            complement(11708..12685)
                     /gene="wzzB"
                     /locus_tag="ACHKTW_003917"
     CDS             complement(11708..12685)
                     /gene="wzzB"
                     /locus_tag="ACHKTW_003917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HFO6464849.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIVTQPDVGQIAGYNNAINVIYGSAAPKVSEIQSMLIGRFSTT
                     FSALAETLDNQEEPEKLTIEPTVKNQSLPLAVSYVGETPEGAQKQLAQYIQQVDDQVN
                     EELEQDLKDNIALQMKNLQDSLKTQEVVAQEQKDLRIRQIQEALQYANQAQVTKPQIQ
                     QTQDVTQDTMFLLGSEALESMIKHEATRPLVFSSNYYQTRQNLLDIDNLDVDKLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(12832..13998)
                     /gene="ugd"
                     /locus_tag="ACHKTW_003918"
     CDS             complement(12832..13998)
                     /gene="ugd"
                     /locus_tag="ACHKTW_003918"
                     /EC_number="1.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HFO6464850.1"
                     /translation="MKITISGTGYVGLSNGILIAQNHEVVALDIVQAKVDMLNKKQSP
                     IVDKEIEEYLATKDLNFRATTDKYDAYKNADYVIIATPTDYDPKTNYFNTSSVEAVIR
                     DVTEINPNAVMVIKSTIPVGFTKSIKERLGIDNLIFSPEFLREGKALYDNLHPSRIVI
                     GERSERAERFAALLQEGAIKQNIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ESLGLNSRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAI
                     VDANRTRKDFIADAILSRKPQVVGIYRLIMKSGSDNFRASSIQGIMKRIKAKGVEVII
                     YEPVMKEDSFFNSRLERDLATFKQQADVIISNRMAEELKDVADKVYTRDLFGSD"
     gene            complement(14247..15653)
                     /gene="gndA"
                     /locus_tag="ACHKTW_003919"
     CDS             complement(14247..15653)
                     /gene="gndA"
                     /locus_tag="ACHKTW_003919"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HFO6464851.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDSEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLTNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQLAGDKAEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
BASE COUNT         3938 a         4038 c         3971 g         3740 t
ORIGIN      
        1 tggttattaa tgaggatgtc gataaaaaag aataaaaaaa ccggagccaa atattcattc
       61 gactccggtt tattattaca aaagatgatt acgctacgtc ttcgtactgc ggaaccgggt
      121 tacggaaact cttggtgacg caagccaggt aaatcagacc gatagccgcc cagatcagac
      181 ccagaaccat tgagctttct tccaggttaa gccacagcgc accaacggtc agcgcaccac
      241 acatcggcag gaacagatac tggaagtgat ctttcagcgt cttattacgc ttctcacgga
      301 tccagaactg cgagatgacc gacaggttaa cgaaggtgaa cgccaccaac gcaccaaagt
      361 taatcagcgc tgtagccatt accaggtcga agttgattgc cagcagggca atcgcgccaa
      421 ccaggatgat gttcatcgcc ggagtacgcc atttcgggtg tacataaccg aagaagcttt
      481 tcgggaatac gccgtcacga cccattacgt acatcagacg cgctacgcct gcatgcgccg
      541 ccataccgga cgccagtacg gtaatggtgg agaagatcag cgcgccaacc tggaacgctt
      601 tacccgcaac gtacagcatg atttcaggct gtgacgcatc cggatcttta aagcgagaga
      661 tatccgggaa gtacagctgc aggaagtaag ttgcaaagat gaagatcatg ccgccaatca
      721 gcgcggtcag gaaaatcgca cgcgggatca cgcgctctgc atctttggtt tcttccgaca
      781 ggttgctgat gccgtcaaag ccggtaaagg agaagcacag gattgtcgcc ccggtaatca
      841 tcgggataac atgcgcatcg ccagaccaga acggacgagt gctcgccagc gtaccagcgc
      901 cttcaccttc aaatacgcca taaacaacca tgcccagaat caccgcgatc agcactacct
      961 gcaacacgac gattacagtg ttgaagttcg ctacggattt cagactacgc aggttaaagg
     1021 cggtcatgaa ggccaccagc gccaccacaa acatccacga tgggatggaa ggcactagag
     1081 cttcaaaata gattttcgcc agcagaatgt tgatcatcgg cgcgaacaga tagtcgagca
     1141 gagaagacca acccaccata aagccgacag tcgggctaat ggatttctgg gcgtaagtgt
     1201 atgcagagcc agcagaagga tagcggcgaa ccagcttccc gtagctcaga gccgtaaaca
     1261 ggatcgcaat caatgcgaac gcataggctg tcggcacatg accatccgtg aggcctgaaa
     1321 cgataccaaa agtatcaaac agcgtcatcg gctgcatata ggcaagaccc atcattacaa
     1381 ccggaactaa cgtaagcgtt ttacgcaatt ccacgcgaga ggtgtttgga gtaacgttat
     1441 gcgacatggt cattctcctt tacggtgata accgtcgcgt aagcaaaaaa ttgccccatt
     1501 tttttggatt cctcagcgac aacaactgtc gatttttagt aaatatctat ccggtacgaa
     1561 gcccggcctc ttggtatgaa tgattggttt gaagcaaaaa ataaccgacg ctgatgaaac
     1621 gtcggttttt agtcattttt tgacagcggc gcattgtgcc taaatggggg ggaaatgaca
     1681 agagaatgag aggcttgtca taataatttt tctttaaatg gctgattttc cgtcatcaga
     1741 ttttgcataa acaccggcga tcgcactatt tgctaaaatt tcatcccgcc accagccgct
     1801 ggcaagccct gcggtctgtt cattccagcc aatccagacg ggttcaaaga gagtttgggc
     1861 gaccactttc ttctcgatta acgcgccact atcgagaaac cgttgcgcca gataacgggg
     1921 taaatagccg cagccgaggc cgctaatttg cagctccagc ttggttttaa aatcgaaaac
     1981 ggtaatggct tcttgttcgt cgagaagttg tgaggccgta gaagcgcctg cctgcgcagt
     2041 atcccccacc acaatcgccc gatatcgctt aataatacga cggtttaacg gctcttcttc
     2101 cagagctaac ggatgatgag gcgcgaccgc aaagacttgt tccagatcgc ccaaccgtga
     2161 aaaaccaaac tcactggaag atggtggctc atgcatcgcg ccaacaataa tatccgctcg
     2221 cccctgagtc agggcatccc aggaaccacc gagtacgcca ttgataaact tcaggcgcgt
     2281 cacgctatga tgttgataaa aagcttcaat cagtggcgca agcagagaaa aaggaaacgt
     2341 gtcgtccacg ccaatcacca gctcgttttc ccagccttca tgcagtttga tcgcctgctt
     2401 ttccagttct cgcacggtat gcagaacttc ccgccctttc tctaataaca tttttccggt
     2461 acgggtgaat ttagcgcggt gaccgctacg atcaagcaat tgaatattaa ggtcgctttc
     2521 cagcttgtga acggtgtaac tcagagcgga cggcgtctta tagagtttgg ccgacgccgc
     2581 cgcaaaactg ccttcttttt ctaaggcatc gaggatcatc aacacatcca gcagtggttt
     2641 catacacgcc cccttgcagt gtatgcggcg tcctgctggc gtgatcgttt actccagcgg
     2701 cattaccagc ggatcgggat actggtattc aaatcccagt tcattacaaa tccgactgcc
     2761 atcaataatc ttacctttgc cgctgtccag actatttctg aactgcggag gctccagccc
     2821 cagtaaacgg gccatctgcg gatagaaaac attacgcgca gggtgagctg gcgcacatat
     2881 attatagatg tgtccgcctt taggtgcctg taacaacaga gtgatagcgc caataacatc
     2941 ttctaaatgg actaaattaa caccatgttc accatcaggc gcggtttttc cggcaaagaa
     3001 gcgtccggga tgacgtcccg gtccgaccag ccccgcaaga cgcagaatat cgaccgaagt
     3061 accgggtaaa ttgtgcagcc agtcttcgag ttcttctaat actcgtccac tgttggttac
     3121 cggattacgc ggggtagttt ctttcaccgt gccttgcgcg tcgccataga cagatgttga
     3181 gctggtaaaa ataatgcggg gaatacgatg ggccagcgcg ctatccacta actcttgtac
     3241 cgcttgtaaa tagaactcat cgccggggcc gctacgacgt gccggaagcg taatgaccag
     3301 cgcatcggca tccatcaggg catccagatc gtcagaatcg caaactagct caggctccat
     3361 gcgaagcaga tagctatcaa tgccactcat tcgggccgct tcgacaccat cttgtgtggt
     3421 tttactccca gtgacttgcc agcctcgcgc tgaaagtgac atcgccagcg gcatgcctaa
     3481 ccaccctaac ccgacaattg cgaccttttt catccgttat ctcctgatac accttagatc
     3541 tataaggcta cgctagcgta tcaaaactga caattcattc tatgaatgaa tctgttcaat
     3601 aatgataacg acatgctgca atgagcagtg aatcgtcggt aaccgcgtat accagacggt
     3661 gctcctctgt aatgcgtcgg gaccagaaac ctgacaaatt atgtttcagg ggttctggct
     3721 tccccttacc ttcaaatggc gttctgcggg tatctttgat aagttcattg atctttttaa
     3781 caattcgctt atctgtttcc tgccagtaca gataatcatc ccatgattcc tcagaccaga
     3841 ttagtttcac tcaataatgt ccttttctgt tcctttgcct gatttcaggc tatcgattga
     3901 gtccatcaat ctccgggcgt tagcggggga acgcagtaga taagccgtct cttccagcga
     3961 gttgtattct tcgagtgaca tcagaacaca agcctctcca ttctgacgag taataaggat
     4021 cggggcatga tcttcaacgg ctttcatcat tgttgccgac aaattctgac gcgcttcgct
     4081 gtagctaatt gtacgcatgt caatctcctc ttttgtacag ttcattgtac aatgatgggc
     4141 gttaattaac tatttattaa ttagtttgta gatcaaggta ttgtcagtga gacgaaaatc
     4201 caggcttcgc tatttttggt gccgtcagct aagaggacag tcctcttagc cccctccttt
     4261 ccccgctcat tcattaaaca aatccatcgc cataaaatat ataaaaaagc ccttgctttc
     4321 taacgtgaaa gtggtttagg ttaaaagaca tcagttgaat aaacattcac agagactttt
     4381 atgacacgcg ttcaatttaa acaccaccat catcaccatc atcctgacta gtctttcagg
     4441 cgatgtgtgc tggaagacat tcagatcttc cagtggtgca tgaacgcatg agaaagcccc
     4501 cggaagatca ccttccgggg gcttttttat tgcgcggttg ataacggttc agacaggttt
     4561 aaagaggaat aacaaaatga cagacaacac tcgtttacgc atagctatgc agaaatccgg
     4621 ccgtttaagt gatgactcac gtgaattgct ggcgcgctgt ggcattaaaa tcaatcttca
     4681 cacccagcgc ctgatcgcga tggcagaaaa catgccgatt gatattctgc gcgtgcgtga
     4741 cgacgacatt cccggtctgg taatggacgg cgtggtagat cttgggatta tcggcgaaaa
     4801 cgtgctggaa gaagagctgc ttaaccgccg cgcccagggt gaagatccgc gctactttac
     4861 cttgcgtcgt ctggatttcg gcggctgccg cctttcgctg gcaacgccgg ttgatgaagc
     4921 ctgggacggc ccgctctcct taaacggtaa acgtatcgcc acctcttatc ctcacctgct
     4981 caagcgttat ctcgaccaga aaggcatctc ttttaaatcc tgcttactga acggttctgt
     5041 cgaagttgcc ccgcgtgccg gactggcgga tgcgatttgc gatctggttt ccaccggtgc
     5101 cacgctggaa gctaacggcc tgcgcgaagt cgaagttatc taccgctcga aagcctgcct
     5161 gatccagcgc gatggcgaaa tggaagaatc caaacagcaa ctgatcgaca aactgctgac
     5221 ccgtattcag ggtgtgatcc aggcacgcga atcaaaatac atcatgatgc acgcaccgac
     5281 cgaacgtctg gatgaagtca tcgccctgct gccaggcgcc gaacgcccaa ctattctgcc
     5341 gctggcaggt gatcaacagc gtgtagcgat gcacatggtc agtagcgaaa ccctgttctg
     5401 ggaaacgatg gaaaaactga aagcgctggg tgccagttca atcctggtcc tgccgattga
     5461 gaagatgatg gagtgatcgc catgagcttt aacacaatca ttgactggaa tagctgtact
     5521 gcggagcaac aacgccagct gttaatgcgc ccggcgatct ccgcttctga aagcattacc
     5581 cgcaccgtta acgatattct cgataacgtg aaaacgcgtg gcgatgaggc cctgcgggaa
     5641 tacagcgcga agtttgataa aaccacggtt accgcgctga aggtgtctgc tgaggagatc
     5701 gccgccgcca gcgaacgcct gagcgacgag ctaaaacagg cgatggcggt ggcagtaaag
     5761 aatattgaaa ccttccacac tgcgcaaaaa ctgccgccag tagatgtaga aactcagcca
     5821 ggcgtacgtt gtcagcaagt cacgcgcccg gtggcttcgg taggtttgta tattcctggc
     5881 ggctccgccc cgctcttctc aacggtatta atgctggcaa ctccggcgcg tattgcgggc
     5941 tgtaaaaaag tggtgctgtg ctcaccgccg ccgattgccg atgagatcct ttatgcggcg
     6001 cagctgtgcg gtgtgcagga cgtgtttaac gtcggcggcg cacaggccat tgccgcactg
     6061 gcgtttggta cggaatctgt gccgaaagtg gacaaaatct tcgggccggg taacgccttt
     6121 gtcaccgaag cgaaacgtca ggtgagccag cgtctggacg gtgcggctat cgatatgccc
     6181 gcaggcccgt cggaagtgtt ggtcattgcc gacagtggcg caacgccgga tttcgtggct
     6241 tctgatttgc tttctcaggc tgaacacggc ccggactcac aggtgatttt actgacgccc
     6301 gatgctgata tggcgcatca agttgccgag gccgtcgaac gccagttggc agaactgccg
     6361 cgtgccgaaa ccgcccgtca ggcacttagc gccagccgcc agatcgtgac taaagattta
     6421 gcgcagtgcg tagagatctc caaccagtac ggcccggagc acctgatcat tcagacccgc
     6481 aacgcccgtg aactggtcga tagcattacc agtgccggtt cggtatttct tggtgactgg
     6541 tcaccggaat cggcaggtga ttacgcctcc ggcaccaacc acgttctacc gacttacggt
     6601 tacaccgcca cctgttccag cctcgggctg gcagatttcc agaagcgcat gaccgtgcag
     6661 gaactgtcga aagaaggctt ttctgcgctg gcttcgacca tagaaacact ggccgccgcc
     6721 gagcgcctga ccgcccataa aaatgccgtt actttgcgtg ttaacgccct taaggagcaa
     6781 gcatgagcac cgtgactatt accgatttag cgcgtgaaaa cgtccgcaac ctgacgccgt
     6841 atcagtcggc gcgtcgtctg ggcggtaatg gcgacgtctg gctgaacgcc aacgaatatc
     6901 ccacagccgt ggagtttcag cttactcagc aaacgctcaa ccgctacccg gaatgtcagc
     6961 cgaaagcagt gattgaaaat tacgcgcaat atgcaggcgt aaaaccggaa caggtgctgg
     7021 tcagccgtgg cgcggacgaa ggtattgaac tactgattcg cgctttttgc gaaccgggta
     7081 aagacgccat cctctactgc ccgccaacgt acggcatgta cagcgtcagc gctgaaacca
     7141 ttggcgtcga gtgccgcaca gtgccgacgc tggaaaactg gcaactggac ttacagggca
     7201 tttccgacaa gctggacggc gtaaaagtgg tctatgtttg cagccccaac aacccgaccg
     7261 ggcaactgat caacccgcag gattttcgca ccctgctgga gttaacgcgc ggtaaggcga
     7321 ttgtggttgc cgatgaagcc tatatagagt tttgtccaca ggcatcgctg gctggctggc
     7381 tggcggaata tccgcacctg gctattttgc gcacactgtc gaaagctttt gctctggcgg
     7441 ggcttcgttg cggatttacg ctggcaaacg aagaggtcat caacctgtta atgaaagtga
     7501 tcgcccccta cccgctctcg acgccggttg ccgacattgc ggcccaggcg ttaagcccgc
     7561 aggggatcgt cgccatgcgt gagcgggtgg cgcaaattat tgcagaacgc gaatacctga
     7621 ttgccgcact gaaagagatc ccctgcgtgg agcaggtttt cgactctgaa accaactaca
     7681 ttctggcgcg ctttaaagcc tccagtgcag tgtttaaatc tttgtgggat cagggcatta
     7741 tcttacgtga tcagaataaa caaccctttt taagcggctg cctgcgaatt accgtcggaa
     7801 cccgtgaaga aagccagcgc gtcattgacg ccttacgtgc ggagcaagtt tgatgagtca
     7861 gaagtatctt tttatcgatc gcgatggaac cctgattagc gaaccgccga gtgattttca
     7921 ggtggaccgt tttgataaac tcgcctttga accgggcgtg atcccggagc tgatgaagct
     7981 gcaaaaagcg ggctacaagc tggtgatgat cactaatcag gatggtctgg gaacacaaag
     8041 tttcccgcag gcggattttg atggcccgca caacctgatg atgcagatct tcacctcgca
     8101 aggcgtgcag tttgatgaag tgctgatttg tccgcacctg cccgccgatg agtgcgactg
     8161 ccgtaagccg aaagtaaaac tggtggagcg ttaccttgct gagcaagcga tggatcgtgc
     8221 caacagttat gtgattggcg atcgcgcgac cgatattcaa ctcgctgaaa acatgggcat
     8281 tactggttta cgctacgacc gcgaaaccct gaactggccg atgattggtg agcaactcac
     8341 taaacgagac cgttacgccc atgtagtgcg caacaccaaa gagacgcaga ttgacgttca
     8401 ggtgtggctg gatcgcgaag gtggcagcaa gattaacacc ggtgttggct tctttgatca
     8461 catgctggat cagatcgcca cccacggcgg tttccgtatg gaaatcaacg tcaaaggcga
     8521 cctctatatc gacgatcacc acaccgtcga agataccggc ctggcgctgg gcgaagcgtt
     8581 aaaaattgcc ctcggcgata aacgcggtat ttgccgcttt ggttttgtgc tgccgatgga
     8641 cgaatgcctt gcccgctgtg cactggatat ctctggtcgc ccgcacctgg aatataaagc
     8701 cgaatttacc taccagcgcg tgggcgatct cagcaccgaa atgatcgagc acttcttccg
     8761 ttcgctctca tacaccatgg gcgtgacgct acacctgaaa accaaaggta aaaacgatca
     8821 ccaccgtgta gagagcctgt tcaaagcctt tggtcgcacc ctgcgccagg ccatccgcgt
     8881 ggaaggcgac accctgccct cgtcgaaagg agtgctgtaa tgaacgtggt gatccttgat
     8941 accggctgcg ccaatctgaa ctcggtgaag tcagccattg cgcgccacgg ttatgaacca
     9001 aaagtcagcc gtgacccgga cgtcgtgttg ctggccgata aactattttt acccggcgtt
     9061 ggcactgcgc aagcagcgat ggatcaggtg cgtgagcgcg agctgtttga tctcatcaaa
     9121 gcctgtaccc aaccggtact gggcatctgc ttagggatgc aactgctggg tcggcgcagc
     9181 gaagagagca acggcgtcga cttgctgggc atcattgacg aagacgtgcc gaaaatgacc
     9241 gactttggtc tgccactgcc gcatatgggc tggaaccgcg tttacccgca ggcaggcaac
     9301 cgcctgtttc aggggattga agacggcgcg tacttttact ttgttcacag ttacgcaatg
     9361 ccggtcaatc cgtggaccat tgcccaatgt aattacggcg aaccgttcac cgcggcggta
     9421 cacaaagata acttctacgg cgtgcagttc cacccggagc gttctggtgc cgctggcgct
     9481 aagttgctga aaaacttcct ggagatgtga tgattattcc ggcattagat ttaatcgacg
     9541 gcaccgtggt gcgtctccat cagggcgatt acggcaaaca gcgcgattac ggtaacgacc
     9601 cgctgccgcg cttacaggat tacgccgcgc agggtgccga agtgttgcat ctggtggatc
     9661 tgaccggggc aaaagatccg gctaaacgtc aaatcccgct gattaaaacc ctggtggcgg
     9721 gcgttaacgt tccggtgcag gttggcggcg gcgtgcgtag cgaagaagac gtggcggcgt
     9781 tactggaagc gggcgttgcg cgcgtggtgg tcggctccac cgcggtgaaa tcaccagaaa
     9841 tggtgaaagg ctggtttgaa cgtttcggtg ccgatgcctt agtgctggcg ctggatgtcc
     9901 gtattgacga gcaaggcaac aagcaggtgg cagtcagcgg ctggcaagag aactcgggcg
     9961 tttcactgga acaactggtg gaaacctatt tgcccgtcgg cctgaaacat gtgctgtgta
    10021 ccgatatctc gcgcgacggc acgctggcag gctctaacgt ctctttatat gaagaagtgt
    10081 gcgccagata tccgcaggtg gcatttcagt cctccggcgg tattggcgac attgatgatg
    10141 tcgctgccct gcgtggtact ggtgtacgcg gcgtaatagt tggtcgtgca ttactggaag
    10201 gtaaattcac cgtgaaggag gccatcgcat gctggcaaaa cgcataatcc catgtctcga
    10261 cgttcgtgat ggtcaggtgg tgaaaggcgt acagtttcgc aaccatgaaa tcattggcga
    10321 tatcgtgccg ctggcaaaac gctacgccga ggaaggtgca gacgaactgg tgttctacga
    10381 tatcaccgct tccagcgatg gccgtgtggt agataaaagc tgggtatctc gcgtggcgga
    10441 agtgatcgac attccgtttt gtgtggcggg tgggattaag tctctggatg acgccgccaa
    10501 aattctttcc tttggcgcgg ataaaatctc cattaactcc cctgcgcttg cggacccgac
    10561 gttaattact cacctggcgg atcgctttgg cgtgcagtgt attgtggtag gtattgatac
    10621 ctggtacgac gctgaaaccg gtaaatatca tgtgaatcaa tataccggcg atgaaagccg
    10681 cacccgcgtc actcaatggg aaacgctcga ctgggtacag gaagtgcaaa aacgcggtgc
    10741 cggagaaatc gtcctcaaca tgatgaatca ggacggcgtg cgtaacggtt acgacctcga
    10801 acaactgaaa aaagtgcgtg aagtttgcca cgtcccgctg attgcttccg gtggcgcggg
    10861 caccatggaa cacttcctcg aagccttccg cgatgccgac gttgacggcg cgctggcagc
    10921 ttccgtattc cacaaacaaa taatcaatat tggtgaatta aaagcgtacc tggcaacaca
    10981 gggcgtggag atcaggatat gttaacagaa caacaacgtc gcgaactgga ctgggaaaaa
    11041 accgacggac tgatgccggt gattgtgcaa cacgcggtat ccggcgaagt gttaatgctg
    11101 ggctatatga acccggaagc cttagacaaa accatcgaaa gcggcaaagt caccttcttc
    11161 tcgcgcacta aacagcgact gtggaccaaa ggcgaaacgt cgggcaattt cctcaatgtg
    11221 gtgagtattg ccccggactg cgacaacgac acgttactgg tgctggcaaa tccaatcggg
    11281 ccaacctgcc acaaaggcac cagcagctgc ttcggcgaca ccgctcacca gtggctgttc
    11341 ctgtatcaac tggaacaact gctcgccgaa cgtaaatctg ccgacccgga aacctcctac
    11401 accgccaaac tgtatgccag cggcaccaaa cgcattgcgc agaaagtggg cgaagaaggc
    11461 gtggaaaccg cactggccgc gactgtacat gaccgctttg agctgaccaa cgaggcgtct
    11521 gatttgatgt atcacctgtt ggtattgttg caggatcagg atctggattt aacgacggtg
    11581 attgagaacc tgcgtaaacg gcatcagtga gttgcggggt aagcggatgc gatattgttg
    11641 tcgcatccgg caaaaaaaaa cgggcaaggt gtcaccaccc tgcccatttt ctttaaaacc
    11701 gaaaagatta cttcgcgttg taattacgca gagcgttacg ccccaacaca atccccgcgc
    11761 caaccatacc gcccagcagc accgccagaa tcaaggtaat tgcctttttc gggctatcgc
    11821 gacgaatagg taacgtcggt ttcattacat agcggtaagc atgaatatca agtttatcaa
    11881 cgtcaagatt gtcgatatcc agcaggtttt gacgagtctg atagtagttt gatgagaaca
    11941 ccaacggacg agtcgcctcg tgcttaatca tcgactccag cgcttcgctc cccaaaagga
    12001 acatagtatc ctgggttacg tcctgtgtct gctgaatctg cggctttgtc acctgcgcct
    12061 gattcgcata ctgcaacgct tcctgaatct gacggatacg cagatctttc tgctcctgcg
    12121 caaccacttc ctgggtcttc agagaatcct gcaagttttt catttgcagc gcgatgttgt
    12181 ctttcaggtc ttgttccagt tcttcattta cctggtcgtc aacttgctgg atatactgcg
    12241 ccagctgttt ctgcgcacct tcaggtgttt cacccacata agaaaccgct aaaggtaatg
    12301 actgattttt aacggtaggc tcaatggtca gtttttcagg ttcttcctga ttatccagcg
    12361 tttctgctaa tgcagaaaat gtcgtgctga aacgaccaat taacatcgac tggatctcag
    12421 acactttcgg cgctgccgaa ccataaatca catttatggc attgttataa ccagcgattt
    12481 gtcccacatc aggttgagta acgatagctg tagaagtcca cttttctttg gcaacggcca
    12541 gataccctat agccagggca atagcgacaa tgacagagat gataattgtc atcttgccac
    12601 gccacaactg caccagtaaa tcaatcaaat caatctgttc cgggtcatgg ttttgcccag
    12661 aaacattatt attttctact ctcatcatta ccctaacgga cgaaataatt ttaagtcgcc
    12721 atagtttagc gataatcgcc atgaatgcta taggataaat gataaaaata atgaattatt
    12781 acaaagggag cataagagaa tcagggcgaa aatcgccctg ataacaggat gttagtcact
    12841 gccaaagaga tcgcgggtgt agactttgtc tgccacatcc ttaagctctt ctgccatacg
    12901 gttggagata atgacgtcgg cttgttgttt gaaggtggcg agatcacgtt ccaggcgaga
    12961 gttgaagaat gagtcttctt tcatcactgg ctcgtatatg atcacttcaa cgcctttcgc
    13021 cttgatacgt ttcataatcc cctgaatgga agacgcacgg aagttatctg atccgctctt
    13081 cataatcaga cgataaatac ccactacttg cggcttgcgt gacaaaatgg catcggcaat
    13141 aaaatcttta cgtgtacggt ttgcatcgac aattgccgaa atcaggttat tcggcacaga
    13201 ctggtagttc gccagtaact gcttggtatc tttcggcaga caataaccac cataaccaaa
    13261 cgacggattg ttgtaatggt tgccaattcg cggatcgaga caaacgcctt cgattatttg
    13321 acgggaattc agacctaaac tttctgcgta gctatccagt tcgttaaagt acgccacgcg
    13381 catcgccagg taggtgtttg caaaaagttt aatcgcttct gcttcagtgg agtcggtaaa
    13441 cagggtaggg atattttgct taatcgctcc ttcctgtaat aacgcagcga aacgttctgc
    13501 gcgttctgaa cgctcaccaa tgacaatacg tgaaggatgg agattatcgt aaagggcttt
    13561 accttcacgg agaaattccg gagagaaaat aaggttatcg atacccagac gttctttaat
    13621 tgatttggtg aaacctaccg gaattgtaga tttaatcacc atgacagcat taggattaat
    13681 ttctgtcaca tcgcgaatga ccgcttccac gcttgaggta ttaaaataat ttgttttcgg
    13741 gtcataatcg gtaggcgtgg caataataac gtaatcggca tttttatacg cgtcatactt
    13801 atctgtcgta gcgcggaaat tgagatcttt agtcgccaga tactcttcaa tctccttatc
    13861 aacaatcggt gactgcttct tgttaagcat gtccactttg gcctgaacga tatccagtgc
    13921 aaccacttcg tgattttgcg caatcagaat accatttgaa agaccaacat aacctgttcc
    13981 tgaaattgtt attttcatta gctctgactt cttccggtta aacatttaga gtggtcatta
    14041 atccaccacc acaacttcat ctactgcggg atttttaacg ctgatgtaga tgaactgtca
    14101 aggcagcgat cctgctgtgc ggcgctgtat tatatcgcgt ttttaactat aaattataaa
    14161 aaaaggccat aacatgccgc tttgtataat aaaaaagccc ggagggtttc tccgggcctt
    14221 gcattgatta attgatttaa atcagattaa tccagccatt cggtatggaa cacaccttct
    14281 ttatcaatgc gcttataagt atgcgcaccg aaatagtcac gctgtgcctg gatcaggttc
    14341 gcaggcagaa cagcggcgcg gtagctgtcg taataggcaa ccgcagcagc gaaggtagga
    14401 acagggatac cgttctgtac tgcataagca acgacatcac gcagcgcctg ctggtagtca
    14461 tcggcaattt gcttgaagta cggagccagc agcaggttag cgatctgcgg attttcggca
    14521 taagcatcgg tgattttctg caggaactgc gcacggatga tgcagccagc acggaaaatc
    14581 ttcgcgattt cgccgtagtt cagatcccag ttgtactctt cagacgccgc acgcagttga
    14641 gagaacccct gagcgtaaga aacgattttg cccagataca gtgcacggcg aactttttcg
    14701 atgaactcag ccttgtcgcc agctagctgc gcttgtgggc cagagagaac tttagacgca
    14761 gcaacgcgct gatctttcag agaagagatg tatcgtgcaa acacagactc ggtaatcagc
    14821 gacagcggtt cgccgagatc cagtgcgctc tggctggtcc atttgcccgt acctttgttt
    14881 gccgcttcat ccaggatcac atcaaccaag tagttaccgt cttcatcttt tttagtgaag
    14941 atgtctttgg taatgtcgat caggtagctg ctcagttcac cgttattcca ctcggtaaag
    15001 gtctgcgcca gttcttcgtt ggtgaggttc agaccacctt taagcagaga ataggcttca
    15061 gcaatcagct gcatatcgcc gtactcaata ccgttgtgaa ccatcttcac atagtgacct
    15121 gcgccatcgg caccaatata ggtaacgcac ggttcgctgt cttcagccac tgcggcgatt
    15181 tttgtcagga tcggcgcaac cagttcatag gcttctttct gcccaccagg cataatggaa
    15241 ggacctttca gcgcgccttc ttcaccaccg gagacaccgg taccaatgaa gttaaagcct
    15301 tctgcagaaa gctcacggtt acgacggatg gtgtcctgga agaaggtgtt accaccatca
    15361 atgatgatgt cgcctttatc gaggtatggc ttgagagaat caatagcagc atccgtgcct
    15421 gcacctgctt tcaccattaa caggatgcga cgaggcgttt ccagagattc aacaaactct
    15481 ttcaccgtat agtaaggaac cagtttcttg cctggatttt cggcaatcac ttcttccgtt
    15541 ttctcacggg aacggttgaa aatagagacg gtataaccac ggctttcgat gttgagcgca
    15601 aggttgcgcc ccatcactgc cataccgacg acgccgatct gttgctttga cattgtttac
    15661 tcctgtcagg gggtgttcac cgcaggg
//
LOCUS       DAZFQD010000123        15629 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.123, whole genome shotgun sequence.
ACCESSION   DAZFQD010000123 DAZFQD010000000
VERSION     DAZFQD010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15629
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.123"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..97
                     /locus_tag="ACHKTW_003920"
     CDS             <1..97
                     /locus_tag="ACHKTW_003920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ZinT/AdcA family metal-binding protein"
                     /protein_id="HFO6464852.1"
                     /translation="QQSLLNEMENWPTYYPYQLSSEEVVEEMMSH"
     gene            440..970
                     /gene="yodB"
                     /locus_tag="ACHKTW_003921"
     CDS             440..970
                     /gene="yodB"
                     /locus_tag="ACHKTW_003921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416483.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HFO6464853.1"
                     /translation="MNRFSKTQIYLHWITLLFVAITYAAMELRGWFPKGSSTYLLMRE
                     THYNAGIFVWVLMFSRLIIKHRYSDPSIVPPPPAWQMKAASLMHIMLYITFLALPLLG
                     IALMAYSGKSWSFLGFNVSPFVTPNSEIKALIKNIHETWANIGYFLIAAHAGAALFHH
                     YIQKDNTLLRMMPRRK"
     gene            complement(1540..1629)
                     /locus_tag="ACHKTW_003922"
     tRNA            complement(1540..1629)
                     /locus_tag="ACHKTW_003922"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1593..1595),aa:Ser,seq:cga)
     gene            1723..2520
                     /gene="mtfA"
                     /locus_tag="ACHKTW_003923"
     CDS             1723..2520
                     /gene="mtfA"
                     /locus_tag="ACHKTW_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HFO6464854.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTCLTEQEQSKLVTLAE
                     RFLQQKRLVPLQGFELDSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFIPLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHRTNDTDSFSATNVH"
     gene            2621..2696
                     /locus_tag="ACHKTW_003924"
     tRNA            2621..2696
                     /locus_tag="ACHKTW_003924"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2654..2656,aa:Asn,seq:gtt)
     gene            3499..8163
                     /locus_tag="ACHKTW_003925"
                     /pseudo
     CDS             3499..8163
                     /locus_tag="ACHKTW_003925"
                     /inference="COORDINATES: protein motif:HMM:NF023351.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
     gene            8344..10992
                     /locus_tag="ACHKTW_003926"
                     /pseudo
     CDS             8344..10992
                     /locus_tag="ACHKTW_003926"
                     /inference="COORDINATES: protein motif:HMM:NF020698.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
     gene            complement(11254..12306)
                     /locus_tag="ACHKTW_003927"
     CDS             complement(11254..12306)
                     /locus_tag="ACHKTW_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HFO6464855.1"
                     /translation="MFLASLLRRIAFSYYDYKAYNFNIEKTDFVVIHIPDQIGDAMAI
                     FPVIRALELHKIKHLLIVTSTINLEVFNALKLEKTKLTLVTMTMQDHATLKEIKDLAK
                     NITQQYGTPDLCIEAMRKKNLKTMIFISQLKAKTNFQVVGLTMKCYSPLCKNASRMDQ
                     NLRAPVPMTWAFMMREAGFPAVRPIYELPLSEDVLDEVREEMRSLGSYIALNLEGSSQ
                     ERTFSLSIAENLIAKIQSETDIPIVIVHGPKGEDKARVLVDCYNNVYRLSLSPSIKRS
                     AAIIKDAYIAITPDTSILHMASAYNTPVVAIYADYKTRWPAMADVSESVVVGQKIDNI
                     SLDEFAKALKSVLARI"
     gene            12621..13937
                     /gene="shiA"
                     /locus_tag="ACHKTW_003928"
     CDS             12621..13937
                     /gene="shiA"
                     /locus_tag="ACHKTW_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate transporter"
                     /protein_id="HFO6464856.1"
                     /translation="MDSTLISTRPDEGTLSLSRARRAALGSFAGAVVDWYDFLLYGIT
                     AALVFNREFFPQVSPAMGTLAAFATFGVGFLFRPLGGVIFGHFGDRLGRKRMLMLTVW
                     MMGIATALIGILPSFSTIGWWAPILLVTLRAIQGFAVGGEWGGAALLSVESAPKNKKA
                     FYSSGVQVGYGVGLLLSTGLVSLISMMTTDEQFLSWGWRIPFLFSIVLVLGALWVRNG
                     MEESAEFEQQQHNQAAAKKRIPVIEALLRHPGAFLKIIALRLCELLTMYIVTAFALNY
                     STQNMGLPRELFLNIGLLVGGLSCLTIPCFAWLADRFGRRRVYITGALIGTLSAFPFF
                     MALEAQSIFWIVFFSIMLANIAHDMVVCVQQPMFTEMFGASYRYSGAGVGYQVASVVG
                     GGFTPFIAAALITYFAGNWHSVAIYLLAGCLISAITALLMKDNQRA"
     gene            14039..15493
                     /gene="amn"
                     /locus_tag="ACHKTW_003929"
     CDS             14039..15493
                     /gene="amn"
                     /locus_tag="ACHKTW_003929"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HFO6464857.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGNYITSGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPLQHEIPYPYVIDGSELTLDRSMSAGLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWHLITADAQGITLV
                     NIGVGPSNAKTICDHLAVLRPDVWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIGIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
BASE COUNT         4256 a         3743 c         3659 g         3971 t
ORIGIN      
        1 acagcaatct ttattaaatg aaatggaaaa ctggccaacg tattatccat atcagttgag
       61 tagcgaagaa gtggtcgagg aaatgatgtc tcattgagta ttctcatgat aacgcctcga
      121 tgccgcttta gtaagtcatc ataactgcca ctggtccatc cacaaacgcc actgaacgca
      181 agctagctac agacacgctc atcactatga cgtgtctgta tattaataag ctaacccgca
      241 ttgagttaac caataacgga ttccatacac aatacggcca acagagaaag atacctgtgc
      301 tcacgccatt gcttatattg gctggttaca atgtgcacta tcaatttttt aaataacaaa
      361 ttcttaaaac cacatctgta taaggatttt ttaaggttcg tgggatagct tgactgtaaa
      421 aattacagga gctacaaaaa tgaaccgatt ctcaaaaact caaatttatt tacattggat
      481 aacgctgctt ttcgttgcaa taacctatgc cgcgatggaa ctccgtggct ggtttcctaa
      541 aggtagtagt acttatctgc tgatgcgaga aacacattac aatgcgggta tattcgtttg
      601 ggtgttaatg ttttcacgcc tgattataaa acaccgttat agtgatcctt ctattgtacc
      661 accgccacct gcctggcaaa tgaaagcggc ttcgctaatg cacatcatgc tttatataac
      721 cttccttgca cttcctctgc tggggattgc tttaatggct tacagtggaa aatcgtggag
      781 tttccttggt ttcaatgtgt ctccctttgt taccccaaac agcgaaatta aagcactgat
      841 aaaaaatatt cacgaaacct gggcaaatat aggttacttt ttaatcgcag ctcacgctgg
      901 cgcagcactc tttcatcact acattcagaa agataatact ctgttacgaa tgatgcctcg
      961 ccgcaaataa gtcgatcccc tgacaaggaa atgtgaacca tagacatcaa cgcgtcataa
     1021 atgtttacgc aagtgcacaa tgcacccttt ccactatcaa caaggaggag cagaaactgt
     1081 gtaaatcgta caatggtaag acttatcaaa aatacaggtt aatttttgta tttaaacctt
     1141 ttttacttgt tagtatgata caaaggcttt caaaaaagct gggaaattca acaactcttg
     1201 gaaattatgg actttgtccc gcggctttaa tctgacggcc gcgttccttt tttgactcaa
     1261 ttattgaccc cttctctacg caacttcagt tcccaccacc aactttgcgg cagtttcata
     1321 ggatgaatgt ctaaaagaat aatggtgagc gataagaaaa cgactgaata actgcacatt
     1381 ttcgctcgaa agcttcctgt cagatccata gcgaatcaag tgctgaatgt cacagtatcg
     1441 aacagaaaac agtgacgatc taacccttca agaatattct acgattgttc tgtttaggaa
     1501 aagcaaggcg ggaagtcggg agataagtca ttgataaagt ggcggagaga gggggatttg
     1561 aacccccggt agagttgccc ctactccggt tttcgagacc ggtccgttca gccgctccgg
     1621 catctctccg ttcagatggt tgccatgatg ccaggaaatt tggcatttta acagtccctg
     1681 tccgtgcaat tttgttcaag tgacgagttt gcgagcaaaa cgatgattaa gtggccctgg
     1741 aaagtacaag aatcagcaca tcaaactgcc cttccctggc aggaagcact atcgatcccc
     1801 cttttaacgt gtctgacgga acaggaacaa agcaaattag tcactcttgc cgaacgtttt
     1861 ttacagcaaa agcggcttgt tcctttacag ggctttgaac tggattcatt aagaagctgc
     1921 cggatagcac ttctattttg cctacccgtt ctggagttag gactggaatg gctggatggt
     1981 tttcatgaag tcttaattta tcctgcgcca tttgtggtcg atgatgaatg ggaagacgat
     2041 atcggtctgg tgcataacca acgtattgtt cagtcaggtc agagctggca gcaagggcct
     2101 atcgttttga actggttgga tatacaagat tcttttgatg cttctggttt taacctgatt
     2161 attcatgaag tcgctcataa gctggacacc cgtaacggcg atcgcgccag cggagttccc
     2221 tttattccgt tgcgtgaggt tgctggctgg gaacacgatc ttcatgctgc aatgaacaac
     2281 attcaggaag aaatcgaatt ggttggcgag aatgcggcga gcattgatgc ttatgctgcc
     2341 agtgatcctg ctgaatgttt tgccgtactt tctgaatatt tctttagcgc cccagaactt
     2401 tttgctcctc gtttcccttc attgtggcaa cgtttctgcc aattttatca acaagatcct
     2461 ttgcagagac tgcatcgcac taatgataca gactcgtttt cggcgacgaa tgttcattaa
     2521 ttaacaactt tgcagattaa ttaaccaatt gaaatgactt atgaaattta gtgttgacag
     2581 acaaggtacc gctaagtaat attcgccccg ttcacacgat tcctctgtag ttcagtcggt
     2641 agaacggcgg actgttaatc cgtatgtcac tggttcgagt ccagtcagag gagccaaatt
     2701 ctaaaaattc gcttcttagc gcaatgtcac taaccttagt tgaacattgt ttattaacgg
     2761 atagcgggtt tttaacatct taagcgccct cgacctttat ggttgagggc gttttgctat
     2821 gaacgccatc accattttcc cctcgattat aaaacttgag ttattcagta gtctcccctc
     2881 ttgcaactca cacccaaaac tgcctaacga aaagttatta attttcaatc atattgctat
     2941 caggatttac attttttcgc tgtgctagaa agggcgcatt tatgttagct cgttcaggga
     3001 aggtaagcat ggctacgaag aagagaagtg gagaagaaat aaatgaccga caaatcttat
     3061 gcgggatggg aattaaacta cgccgcttaa ctgcgggtat ctgtctgata actcaacttg
     3121 cgttccctat ggctgcggca gcacaaggtg tggtaaacgc cgcaacccaa caaccagttc
     3181 ctgcaccgta ttgccattgc aaatgccaat acggtgccct acacccttgg agcgctggaa
     3241 tcggcccaaa gcgttgccga acgtttcggt atttcggtgg ctgagttacg caaactcaac
     3301 caatttcgta cgtttgctcg aggttttgat aatgtccgcc agggtgatga actggatgtc
     3361 ccggcacaag ttagtgaaaa taatttaacc ccgccaccgg gtaatagcag tggcaacctt
     3421 gagcaacaga tagccagtac ttcacagcaa atcgggtctt tgctcgccga ggatatgaac
     3481 agcgaacaag cggcaaatat ggcgcgtgga tgggcctctt ctcaggcttc aggcgcaatg
     3541 acagactggt taagccgctt cggtaccgca agaatcacgc tgggcgtgga tgaagatttt
     3601 agcctgaaga actcccagtt cgattttctc catccgtggt atgaaacgcc tgataatctc
     3661 tttttcagtc agcatactct ccatcgtact gacgagcgta cgcagattaa caacggcttg
     3721 ggttggcgtc atttcactcc cacatggatg tcgggcatca acttcttttt cgaccacgat
     3781 cttagccgtt accactcccg cgccggcatt agcgcggagt actggcgcga ctatctaaaa
     3841 ttaagcagta acggctattt gcgactgacc aactggcgca gcgcacctga gctggacaac
     3901 gattatgaag cccgcccggc caatggctgg gatgtacgcg cagaaggctg gttacccgcc
     3961 tggccgcacc ttggcggtaa actggtctat gaacagtatt atggcgatga agtggccctg
     4021 ttcgataaag acgatcggca aagtaatcct catgccataa ccgctggact taactatacc
     4081 cccttcccgc tgatgacctt cagcgcggag caacgccagg gtaaacaggg cgaaaatgac
     4141 acccgttttg ccgtcgattt tacctggcta cctggcagcg caatgcagaa acagcttgac
     4201 ccgaatgaag tcgctgcacg gcgtagcctt gcaggcagcc gttatgatct ggtggatcgc
     4261 aacaacaata tcgttctgga atatcgcaaa aaagaactgg ttcgcctgac cctgacagac
     4321 cccgtgacag ggaagtcagg agaagtgaaa tcactggttt cgtcgctaca aaccaaatat
     4381 gccctgaaag gctataacgt cgaagccacc gctctggaag ctgccggtgg taaagtggtt
     4441 acaacgggta aagatattct ggttaccctg ccggcgtacc ggttcaccag tacgccagaa
     4501 accgataaca cctggccgat tgaagtcacc gctgaagatg tcaaaggcaa tttgtcgaat
     4561 cgtgaacaga gcatggtggt cgttcaggca cctacgctaa gccagaaaga ttcctcagta
     4621 tcattaagta cccaaacatt gaacgcggat tcccattcaa ccgccacact aacttttatt
     4681 gcgcatgatg cagcaggtaa tcctgttatc gggctggtgc tttcgacgcg tcacgaaggt
     4741 gttcaggaca tcaccctgtc ggactggaaa gataatggtg acggaagcta tacccaagtc
     4801 ctgaccacag gagcgatgtc tggcacgctg acgcttatgc cacagctgaa cggtgtggat
     4861 gcggctaaag cccccgccgt ggtgaatatc atttctgttt cgtcatcccg aactcactcg
     4921 tcaattaaga ttgataagga ccgttatctc tccggcaatc ctatcgaggt gacggtagaa
     4981 ctgagagatg aaaatgacag acctgttaag gagcaaaaac agcaactgaa taccgcagtc
     5041 agcatcgaca acgtgaaacc aggagtcact acagactgga aagaaaccgc agatggcgtc
     5101 tataaggcga cctataccgc ctataccaga ggcagtgggc ttactgcgaa gctattaatg
     5161 caaaactgga atgaagattt acataccgct ggatttatca tcgacgccaa cccgcagtca
     5221 gcgaaaattg cgacattatc tgccagcaat aatggtgtgc tcgccaatga gaatgcagca
     5281 aacaccgtct cggtcaatgt cgctgatgaa ggaagcaacc caatcaatga tcataccgtc
     5341 acgtttgcgg tattaagcgg atcggcaact tccttcaaca atcaaaacac cgcaaaaacg
     5401 gatgttaatg gtctggcgac ttttgatctg aaaagtagta agcaggaaga caacacggtt
     5461 gaagtcaccc ttgaaaatgg cgtgaaacaa acgttaatcg tcagttttgt cggcgactcg
     5521 agtaccgcgc aggttgagct gcagaagtcg aaaaatgaag tggtcgctga cggcaatgat
     5581 agcgccacaa tgaccgcgac agtccgggat gcaaaaggca acctgctcaa tgacgtcaag
     5641 gtcactttca atgttaattc agcagaggcg aaactgagcc aaacagaagt gaatagccac
     5701 gacgggatcg ccacagctac gctgaccagt ttgaaaaatg gtgattatag ggttacggcc
     5761 tctgtgagct ctggttccca ggctaatcaa caggtgaatt ttatcggtga tcaaagtact
     5821 gctgccctga ccctcagtgt gccttcaggt gatatcaccg tcaccaacac agctccgcta
     5881 catatgactg caaccttgca ggataaaaat ggcaacccat taaaagacaa agaaatcacc
     5941 ttctctgtgc caaacgacgt cgcaagtcgg ttctcgatta gcaacagcgg aaaaggcatg
     6001 acggatagca acgggactgc aatcgcctcc ctgaccggca cgttagcggg cacgcatatg
     6061 atcacggctc gtctggctaa cagcaatgtc agcgatacac agccaatgac gtttgtggcg
     6121 gataaagaca gagcggttgt cgttctgcaa acatcgaaag cggaaatcat tgggaatggc
     6181 gtggatgaga caactctgac agcaacagtg aaagatcctt ttgataacgt ggttaaaaat
     6241 ctgtcagtag tcttccgcac ctcccccgca gacacgcaac tgagtctgaa ggcgcttaat
     6301 actaatgaga acggtattgc tgaagttacc cttaaaggca cggttttggg tgttcataca
     6361 gccgaagcca tactgcttaa cggtaaaagc gatacgaaaa tcgtcaatat tgtgcccgat
     6421 accagcaacg cgcaggtcac cctgaacatc ccggctcaac aggtggtgac gaataacagc
     6481 gacagcgtac agctgacggc gatggtgaaa gatccgtcga atcatccggt ggcgggaata
     6541 acggtgaact tcaccatgcc acaggacgtt gcggcaaact ttacccttga aaataacggt
     6601 attgccatca ctcaggccaa tggcgaagcg catgtcacgc tcaaaggtaa aaaagcgggc
     6661 atgcatacgg ttaccgcaac gttgggtaac aataatgcca gcgatgcgca accagtcaca
     6721 ttcgtggcgg ataaggacag cgcagttgtc gttctgaaaa catcgaaagc ggaaatcatt
     6781 gggaatggcg tggatgagac gactctgacg gcaacagtga aagatccttt tgataacgta
     6841 gtaaaagatc taccggtcac cttcagtacc aacccggcag atactcaact tagtcagagc
     6901 acaagcaata ctaacgacag tggtgttgct gaagttaccc ttaaggacac ggttttgggt
     6961 gttcatacag tcgaagccac gctgcttaac ggcaatggct atacgacgac agtcaatatt
     7021 gcgcccgatg ccagcaacgc gcaggtcacc ctgaacatcc ctgctcaaca ggtggtgacg
     7081 aataacagtg acagcgtgca gcttacggcg atggtgaaag atccgtcgaa tcatccggtg
     7141 gcggggataa cggtcaactt caccatgcca caggacgttg cggcaaactt taccctcgaa
     7201 aataacggca ttgccatcac ccaagccaat ggggaagcgc atgtcacgct gaaaggtaaa
     7261 aaagcgggta cgcatacggt taccgcaacg ctgggtaata acaataccag tgattcgcag
     7321 ccggtaacgt ttgtggcgga caaaacctcg gctcaggttg tcctgcagat gtcaaaagat
     7381 gagatcacag gtaatggcgt cgataacgca acgctaactg caacggttaa agatcagttc
     7441 gacaatgagg tgaataatct tccggtaaca ttcagctcag cctcttcagg actcaccctg
     7501 accccgggag taagtaatac caatgagtct ggcatcgcgc aggccactct cgcaggcgtt
     7561 gcctttggtg agcagacggt cactgcatca ctggctaata atggtgccag cgacaacaaa
     7621 actgtgcatt ttattggcga cacagcggcg gcaaaaatta tcgagttgac gcctgtccca
     7681 gacagcataa tcgccggtac cccgcagaac agctccggca gcgtcatcac cgccacagtc
     7741 gttgataata atggctttcc ggtgaaaggt gtgactgtga acttcaccag cagaacaaac
     7801 tctgccgaaa tgacgaatgg cggccaagcc gtaacgaacg aacagggtaa ggctaccgtc
     7861 acttatacca atacccgctc ctcgatagaa tcaggagcga gaccggatac cgttgaggcc
     7921 agtctggaaa atggtagctc cacgcttagc acatcaatta atgtcaacgc tgatgcgtct
     7981 acggcacatc tcaccttgct acaggcactt tttgatacag tctccgcagg cgacactacc
     8041 aatctgtata ttgaggtgaa ggataattac ggcaacggtg taccccagca ggaggtaacc
     8101 ctcagagtat caccaagtga aggcgtgacc cccagtaata acgctatata tactaccaac
     8161 cacgacggca atttttacac aagctttacc gctacaaaag ccggggttta tcaagtgacg
     8221 gcaaccctcg aaaatggcga ttcgatgcaa caaacagtga cctatgtgcc gaacgtcgcg
     8281 aatgccgaaa tcacgctggc agcctcgaag gatccggtga ttgccgacaa taacgatctc
     8341 acgacactaa cagcaacagt cgctgataca gagggcaatg cgatagccaa cactgaagta
     8401 acatttactc tgccggaaga tgtgagggcg aacttcacgc tgagcgatgg cggtaaagcg
     8461 attactgata ctgaaggcaa agcgaaagtc acgctgaaag gtataaaagc aggcgctcat
     8521 actgttacag catcgatggc tggcagtaag agtggacaac tggtggtgaa ctttactgcg
     8581 gatacgctca ctgcgcaggt taatcttaac gttaccgagg acaatttcat cgccaataac
     8641 attgggatga ccaagctgca ggcaacagtg actgatggaa acggcaaccc attcgccaat
     8701 gaggcggtga cattcacgct accggcagat gtgagcgcaa gcttcactct cggacaaggc
     8761 ggttccgcca ttactgatat caacggcaag gctgaagtta cactgagcgg taccaaatcc
     8821 ggcacctatc ccgtgacagt tagcgtgatc aattatggtg tcagtgatac gaaacaggtg
     8881 actttgattg ccgatgctgg taccgcaaca ctagcctcct taacctctgt atactcattc
     8941 gtcgtcagca cgaccgaggg cgcgaccatg actgcaagcg tcactgacgc taacggcaac
     9001 ccggtagaag gcataaaagt taatttccgc ggaacctccg tcacgctaag cagcaccagc
     9061 gttgaaacgg atgatcaggg tttcgctgaa attcttgtga caagcaccga agtcggactg
     9121 aaaacagttt cagcctctct ggcagataaa cctactgaag tcatatcgcg attactgaat
     9181 gcaaaagcag atattaattc tgcaacgatt accagtctgg agatacctga aggtcagcta
     9241 atggtcgcac aagacgtagc agttaaagct cacgtcaacg accagtttgg caacccgatt
     9301 cttaatgaat ctgtaacatt cagtgcagag ccaccagagc acatgaccat cagccaaaat
     9361 attgtctcta ctgatacgca tggtatagcc gaggtctcca tgacgcccga aagaaacggt
     9421 tcgtatatgg tgaaagcatc cctggcgaat ggagcctcac ttgagaaaca actggaggct
     9481 attgatgaaa aactgacact cacggcgtcc agtccgctta tcggtgtcta tgcccctaca
     9541 ggcactactc tgacggcaac gctaacctct gcaaatggca ctccagtgga gggtcaggtc
     9601 atcaacttta gcgtaacgcc agaaggggcg acgttaagtg gcggaaaagt gagaactaac
     9661 tcttcaggtc aggctccggt cgttctgacc agcaataaag tcggtacata tacggtgact
     9721 gcatcgttcc ataacggcgt aacaatacag acacagacaa ccgtgaaagt cactggcaac
     9781 tcaagcaccg cacatgttgc tagctttatc gctgatccat cgactatcgc cgccaccaac
     9841 agtgatttaa gtaccttaaa ggcaacggtt gaggatggca gtggtaacct gatcgaaggt
     9901 ctcactgtgt acttcgcctt aaaaagcggc tctgccacat taacgtcatt aacagcggtg
     9961 accgatcaaa acggaatcgc gacaacaagc gtgaaaggag cgatgacagg tagcgtcacg
    10021 gtaagcgcag tcacgaccgc tggtggaatg caaacagtag atataacgct ggtggcaggc
    10081 ccggcagacg cctcgcagtc cgtccttaag aacaatcggt cctcactgaa aggggacttt
    10141 accgatagtg ctgagctaca tcttgttttg cacgatatat caggcaatcc gatcaaagtt
    10201 tctgaaggga tggaatttgt gcaatcaggt actaacgtgc cctatatgaa aattagcgca
    10261 attgattaca gtctaaatat caacggtgat tacaaagcca ctgttacagg cggcggagag
    10321 ggtatcgcaa cgctgatccc tgtattgaat ggtgttcatc aagctggtct gagtaccaca
    10381 atacaattca ctcgcgcaga agacaaaata atgagcggta cagtatcagt caatggtact
    10441 gacctaccga caactacatt cccttcgcag gggttcaccg gggcgtatta tcagttgaat
    10501 aatgacaact ttgccccagg aaaaacggcg gctgattatg agttttcaag ctctgcctcc
    10561 tgggtcgatg ttgatgctac cggtaaagtg acatttaaaa atgtcggcag caattgggaa
    10621 aggattacgg cgacgccaaa atcaggaggc cctagctatg tatacgaaat ccgtgtgaag
    10681 agttggtggg tgaactccgg cgatgctttc atgatataca gccttgctga aaatttttgc
    10741 agtagcaatg gctacacact tccccgtgca gatcatttaa accatagtcg ttcccgaggc
    10801 atcgggtcac tgtacagtga atggggagat atggggcatt acacgactga cgctggtttt
    10861 caatcaaata tgtattggtc atctagtccc gcaaactcaa gcgaacaata cgtagtttcc
    10921 ctggcaacag gtgatcaaag cgtatttgaa aagcttgggt ttgcttatgc gacatgttat
    10981 aaaaacctgt gattttccgt tataatttct taaagagtgc tccattcgga gcactctttt
    11041 ttacattccc tctatagagc ttgcccttct ctcacagaaa atgaataaat aaaactgctg
    11101 caccgcaaga atcgcgttcg atgtgactat gatcgtatcc ggtgagtaca gctttgatcc
    11161 aaaggctggt tttctgtgat gattgaccag cctcagagta ttgatgacac cgccgttcat
    11221 aacagatcgg cattactatg cataagtact gtctcatatt ctcgccaaaa cactttttaa
    11281 tgcctttgcg aattcatcca gacttatatt gtcaattttt tgcccaacga cgactgactc
    11341 cgaaacatcg gccattgcgg gccatcgcgt tttgtaatca gcataaattg caacaacggg
    11401 agtattatag gcacttgcca tatgtaatat tgaggtgtca ggagttattg cgatataagc
    11461 atcttttatg attgctgctg aacgtttaat cgagggtgat aaggataaac ggtagacatt
    11521 attataacaa tccactaata ccctggcttt atcttcccct tttggtccat gaacgatcac
    11581 tattggtata tctgtttcac tttgaatttt tgctattaga ttttctgcaa tcgataatga
    11641 aaatgtacgt tcctgcgagc taccttctaa attaagcgca atgtaagatc ctaacgatcg
    11701 catttcctcg cgcacctcat cgagtacatc ctcacttagt ggcaattcat atattggcct
    11761 gactgctgga aaacccgcct cacgcatcat aaatgcccat gtcatgggta caggagcccg
    11821 gagattctga tccatccggg acgcgttctt gcacaaaggg gagtagcact tcatggttaa
    11881 accaacaacc tgaaaattcg tttttgcttt caactgactg ataaatatca tcgttttcag
    11941 gttcttttta cgcatcgcct ctatacaaag atccggcgta ccgtattgct gtgttatgtt
    12001 ctttgctaaa tcttttattt cttttaatgt tgcgtgatcc tgcatagtca ttgtgactaa
    12061 tgttaattta gtcttttcaa gtttaagtgc attaaacact tctaaattaa ttgtcgacgt
    12121 aacaattaaa agatgcttaa ttttatgcaa ttcaagcgcc cgaataacag gaaagatggc
    12181 catagcatcg ccaatctggt cgggaatatg gatgacaaca aagtctgttt tttcaatatt
    12241 gaaattataa gctttataat cgtagtaact aaatgcaata cgtctcaaca atgatgctaa
    12301 aaacatacct aacctcgcct ccctactggt tataatgcaa tgcagtctat cagactcatc
    12361 agggtgccat tttgtgcata tgcggacttt tatgtttcat atctctaacc tgtgggtcct
    12421 ctgcttaatc cttaaacaac accagcaact cctgcgcttt catcttccat cgaatttttc
    12481 atgttgccgc taatcagcca taaaatcatt tgcagatgcg ctctgtcgag gtagtctcat
    12541 aaggttcgtt tatagatcga cggcaatgtg agttaccttt tccatactaa ttataaaaag
    12601 acagtacaaa caggatcatt atggactcca cgctcatctc cactcgtccc gatgaaggga
    12661 cgctttcgtt aagtcgcgcc cgacgagctg cgttaggcag cttcgctggt gccgtcgtcg
    12721 actggtatga ttttttactc tatggcatca ccgccgcact ggtgtttaat cgcgagtttt
    12781 tcccgcaagt aagcccggcg atgggaacgc tcgccgcatt tgctaccttt ggcgtcggat
    12841 tccttttccg tccgctcggc ggtgtcattt tcggtcactt tggcgaccga ctgggacgta
    12901 agcgcatgtt aatgctgacc gtctggatga tgggcatcgc gacagccttg attggtattc
    12961 ttccttcatt ctcgaccatt gggtggtggg cacctatttt actggtgacg ctgcgtgcca
    13021 ttcagggatt tgccgtcggc ggcgaatggg gaggcgcggc gttgctttcc gttgaaagtg
    13081 caccaaaaaa taaaaaagcc ttttacagta gcggtgtaca agttggctac ggtgtaggtt
    13141 tactgctttc aactggactg gtttcattga tcagtatgat gacgactgac gaacagtttt
    13201 taagctgggg ctggcgcatt cctttcctgt ttagcatcgt actggtactg ggagcattgt
    13261 gggtgcgtaa tggcatggag gagtccgcgg aatttgaaca acagcaacat aatcaagcgg
    13321 ccgcgaaaaa acgcattccg gttatcgaag cactattacg gcatcctggt gctttcctga
    13381 agattattgc gctacgactg tgcgagttgc tgacgatgta catcgttacc gccttcgcac
    13441 ttaattattc aacccagaat atggggttac cgcgcgaact tttcctcaat attggtttgc
    13501 tggtaggtgg attaagctgc ctgacaattc cctgttttgc ctggcttgcc gatcgttttg
    13561 gtcgccgcag ggtttatatc acaggcgcgt taatcggaac gttgagcgca tttcctttct
    13621 ttatggcgct tgaagcacaa tccattttct ggatagtttt cttctccata atgctggcaa
    13681 acattgcgca tgacatggtg gtgtgtgtgc aacaaccgat gtttaccgaa atgtttggtg
    13741 ccagttatcg ctatagtggc gctggagtcg gttatcaggt tgccagtgtg gttggcggtg
    13801 gatttacacc ttttattgcc gctgcactca tcacttactt tgccgggaac tggcatagcg
    13861 tcgccattta tttgctggct ggatgcctga tttctgcaat aaccgctttg ttgatgaaag
    13921 acaatcaacg cgcttgatag tctggcgaag atcatccgat cttcgcctta cacttttgtt
    13981 tcacattcct gtgacatact atcggatgtg cggtaattgt atggaacagg agacacacat
    14041 gaataataag ggctccggtc tgaccccagc tcaggcactg gataaactcg acgcgctgta
    14101 tgagcaatct gtagtcgcat tacgcaacgc cattggcaac tatattacaa gtggcgaatt
    14161 acctgatgaa aacgcccgca aacaaggtct ttttgtctat ccatcactga ccgtaacctg
    14221 ggacggtagc acaaccaatc cccccaaaac gcgcgcattt ggtcgtttta cccacgcagg
    14281 cagctacacc accacgatta ctcgccctac tctctttcgt tcgtatctta atgaacaact
    14341 tacgttgctg tatcaggatt atggtgcgca tatctctgtg caacccttgc agcatgaaat
    14401 cccttatcct tatgtcatcg atggctctga attgacactt gatcgctcaa tgagcgctgg
    14461 gttaactcgc tacttcccga caacagaact ggcgcaaatt ggagatgaaa ctgcagacgg
    14521 catttatcat ccaactgaat tctccccgct atcgcatttt gatgcgcgcc gagtcgattt
    14581 ttccctcgcg cggttgcgcc attataccgg tacgccagtt gaacattttc agccgttcgt
    14641 cttgtttacc aactacacac gttatgtgga tgaattcgtt cgttggggat gcagccagat
    14701 cctcgatcct gatagtccct acattgccct ttcttgtgct ggcgggaact ggatcaccgc
    14761 cgaaaccgaa gcgccagaag aagccatttc cgaccttgca tggaaaaaac accagatgcc
    14821 agcatggcat ttaattaccg ccgatgctca ggggattact ctggtgaata ttggcgtggg
    14881 accgtcaaat gctaaaacca tctgcgatca tctggcagtg ctacgcccgg atgtctggtt
    14941 gatgattggt cactgtggcg gattacgtga aagtcaggcc attggcgatt atgtacttgc
    15001 acacgcttat ttacgcgatg accacgttct tgatgcggtt ctgccgcccg atattcctat
    15061 tccgagcatt gctgaagtgc aacgtgcgct ttatgacgcc accaagctgg tgagcggcag
    15121 gcccggtgag gaagtcaaac agcggctacg tactggtact gtggtaacca cagatgacag
    15181 gaactgggaa ttacgttact cagcttctgc acttcgtttt aacttaagcc gggccgtagc
    15241 aattgatatg gaaagtgcaa ccattgccgc gcaaggatat cgtttccgcg tgccatacgg
    15301 gacactactg tgtgtttcag ataaaccatt acatggtgag attaaacttc ccggccaggc
    15361 taaccgtttt tatgaaggcg ctatttccga acatctgcaa attggcattc gggcgatcga
    15421 tttgctgcgc gcagaaggcg accgactgca ttcgcgtaaa ttacgaacct ttaatgagcc
    15481 gccgttccga taataagttc tgaaccttct tatcagaaat agatgtgaag gagtaagtaa
    15541 gacacctggc aaatagcctg caataatcgt gggctgtttg cttccttggg cggatacgag
    15601 ttttattatc gtcttaatga tttccacat
//
LOCUS       DAZFQD010000124        15568 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.124, whole genome shotgun sequence.
ACCESSION   DAZFQD010000124 DAZFQD010000000
VERSION     DAZFQD010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15568
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.124"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1276
                     /locus_tag="ACHKTW_003930"
     CDS             <1..1276
                     /locus_tag="ACHKTW_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO6464858.1"
                     /translation="SYPTGVENHGGSLRIWFHYNGKRVRENLGVPDTAKNRKIAGELR
                     TSVCFAIRMGSFDYATQFPNSPNLKHFGLGKREITVKALSEKWLDLKKIEICANALNR
                     YQSVIKNMLPMLGEKKLVSSITKEDLLFARRDLLTGYQKLSNGKISSIKGRSVVTVNY
                     YMTTIAGMFQFATDNGYTSGNPFNGLAPLKKSKVKPDPLTRDEFIRFIEACRHQQTKN
                     LWILAVYTGIRHGELVSLAWEDIDLKARTITIRRNYTKLGEFTPPKTDAGTGRTIHLV
                     QPTIDALKSQAEMTMLGKQHSVEVKQREYGRSTVHKCTFVFSPQVIKQRQFSGPHYKV
                     DSIRESWTSILKRAGLRHRKSYQSRHTYACWSLAAGANPSFIASQMGHTNAQMVFNVY
                     GAWMKDNNHEQIELLNKRLSESVPCMPHKKAG"
     gene            1297..1998
                     /gene="mlrA"
                     /locus_tag="ACHKTW_003931"
     CDS             1297..1998
                     /gene="mlrA"
                     /locus_tag="ACHKTW_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MlrA"
                     /protein_id="HFO6464859.1"
                     /translation="MSLPLIPVTLRAWQRRYGLLKPQRTDGGHRLFNDADIDRIREIK
                     RWIDNGVQVSKVKMLLSNENVDVQNGWRDQQETLLTYLQSGNLHSLRTWIKERGQDYP
                     AQTLTTHLFIPLRRRLQCQQPTLQALLAILDGVLINYIAICLASARKKQGKDALVVGW
                     NIQDTTRLWLEGWIASQQGWRIDVLAHSLNQLRPELFEGRTLLVWCGENRTSAQQQQL
                     TSWQEQGHDIFPLGI"
     gene            2058..2165
                     /gene="yohO"
                     /locus_tag="ACHKTW_003932"
     CDS             2058..2165
                     /gene="yohO"
                     /locus_tag="ACHKTW_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HFO6464860.1"
                     /translation="MRIAKIGVIALFLFMAFGGIGGVMLAGYTFILRAG"
     gene            complement(2146..2877)
                     /gene="yehW"
                     /locus_tag="ACHKTW_003933"
     CDS             complement(2146..2877)
                     /gene="yehW"
                     /locus_tag="ACHKTW_003933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HFO6464861.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPVLQATLAGLGAIDASVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(2882..3808)
                     /gene="yehX"
                     /locus_tag="ACHKTW_003934"
     CDS             complement(2882..3808)
                     /gene="yehX"
                     /locus_tag="ACHKTW_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HFO6464862.1"
                     /translation="MIEFSHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGEIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMTLLGLESNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVRLLSLRSVADYVRREERAEGEALVEEMTLRDALS
                     LFVARGCEVLPVVNTQGQPCGTLHFQDLLVEA"
     gene            complement(3801..4958)
                     /gene="yehY"
                     /locus_tag="ACHKTW_003935"
     CDS             complement(3801..4958)
                     /gene="yehY"
                     /locus_tag="ACHKTW_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HFO6464863.1"
                     /translation="MTYLRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGRHLWQL
                     WPQTIWMLVGVGCAWLTACFVPAKKGSICALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGSGFWLAAALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGKLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLIDALFDLLIALLKVKRND"
     gene            complement(4965..5882)
                     /gene="osmF"
                     /locus_tag="ACHKTW_003936"
     CDS             complement(4965..5882)
                     /gene="osmF"
                     /locus_tag="ACHKTW_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HFO6464864.1"
                     /translation="MPLLKLWAGSLVMLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVVREAITSGELDIYPEYTGNGAFFFKDENDAAWKNAQ
                     QGYEKVKKLDSEHNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLQEGGTF
                     KLAASAEFIERADALPAFEKAYGFKLGQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGPVAALGLQTLSDPQGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWAK"
     gene            complement(6093..8390)
                     /gene="bglX"
                     /locus_tag="ACHKTW_003937"
     CDS             complement(6093..8390)
                     /gene="bglX"
                     /locus_tag="ACHKTW_003937"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HFO6464865.1"
                     /translation="MKWLCSVGIAVSLALQPALADDLFGNHPLTPEARDAFVTELLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSTMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMEELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNSV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQSQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPMSFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            8586..10301
                     /gene="dld"
                     /locus_tag="ACHKTW_003938"
     CDS             8586..10301
                     /gene="dld"
                     /locus_tag="ACHKTW_003938"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_process: GO:0006089 - lactate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFO6464866.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDVVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARINEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            complement(10339..11271)
                     /gene="pbpG"
                     /locus_tag="ACHKTW_003939"
     CDS             complement(10339..11271)
                     /gene="pbpG"
                     /locus_tag="ACHKTW_003939"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416638.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HFO6464867.1"
                     /translation="MPKFRVSLFSLALMLAVPFAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(11445..12032)
                     /gene="yohC"
                     /locus_tag="ACHKTW_003940"
     CDS             complement(11445..12032)
                     /gene="yohC"
                     /locus_tag="ACHKTW_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HFO6464868.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLALTVILWGYGYRLF"
     gene            12202..12780
                     /gene="yohD"
                     /locus_tag="ACHKTW_003941"
     CDS             12202..12780
                     /gene="yohD"
                     /locus_tag="ACHKTW_003941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFO6464869.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHTLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDNQA"
     gene            complement(12910..13671)
                     /gene="yohF"
                     /locus_tag="ACHKTW_003942"
     CDS             complement(12910..13671)
                     /gene="yohF"
                     /locus_tag="ACHKTW_003942"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO6464870.1"
                     /translation="MAQVAIITASDSGIGKECALLLAQQGFDIGITWHSDEEGAKDTA
                     REVVSHGVRAEIVQLDLGKLPEGAQALEKLIQRLGRIDVLVNNAGAMTKAPFLDMAFD
                     EWRKIFTVDVDGAFLCSQIAARQMVKQGQGGRIINITSVHEHTPLPDASAYTAAKHAL
                     GGLTKTMALELVRHKILVNAVAPGAIATPMNGMDDSYVKPDAEPSIPLRRFGATHEIA
                     SLVAWLCSEGANYTTGQSLIVDGGFMLANPQFNPE"
     gene            complement(13724..15160)
                     /gene="mdtQ"
                     /locus_tag="ACHKTW_003943"
     CDS             complement(13724..15160)
                     /gene="mdtQ"
                     /locus_tag="ACHKTW_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
                     /protein_id="HFO6464871.1"
                     /translation="MNRDSFYPAIACFPLLLMLAGCAPMHETRQALSQQTPAAQVDTA
                     LPTALKNGWPDSQWWLEYHDNQLTSLINNALQNAPDMQVAEQRIQLAEAQAKAIATQD
                     GPQIDFSADMERQKMSAEGLMGPFALNDPAAGTTGPWYTNGTFGLTAGWHLDIWGKNR
                     AEVTARLGTVKARAAEREQTRQLLAGSVARLYWEWQTQAALNTVLQQIEKEQNTIIAT
                     DRQLYQNGITSSVEGVETDINASKTRQQLNDVAGKMKIIEARLSALTNNQTKSLKLKP
                     VALPKVASQLPDELGYSLLARRADLQAAHWYVESSLSTIDAAKAAFYPDINLMAFLQQ
                     DALHLSDLFRHSAQQMGVTAGLTLPIFDSGRLNANLDIAKAESNLSIASYNKAVVEAV
                     NDVARAASQVQTLAEKNQHQAQIERDALRVVGLAQARFNAGIIAGSRVSEARIPALRE
                     RANGLLLQGQWLDASIQLTGALGGGYKR"
     gene            15384..15467
                     /gene="yohP"
                     /locus_tag="ACHKTW_003944"
     CDS             15384..15467
                     /gene="yohP"
                     /locus_tag="ACHKTW_003944"
                     /inference="COORDINATES: protein motif:HMM:NF011345.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YohP"
                     /protein_id="HFO6464872.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
BASE COUNT         3813 a         4242 c         3885 g         3628 t
ORIGIN      
        1 atcatacccg acaggcgttg aaaaccatgg aggatcactc cgtatatggt ttcactataa
       61 tggcaaacgt gtcagagaaa acctcggtgt tcctgacacc gccaaaaacc ggaagatcgc
      121 aggtgaactt cgcacttccg tttgttttgc aatcagaatg gggagtttcg actacgctac
      181 acagttccct aattccccta acctgaaaca ctttggtctg ggaaaaagag agataaccgt
      241 taaggcactt tcggaaaaat ggttggacct taagaaaatt gagatttgtg cgaatgcact
      301 taatcgttac cagtcagtaa ttaaaaacat gttgcctatg ttgggtgaga aaaaactggt
      361 ttcatccata acaaaagagg atttactttt cgcaaggaga gatttgttga ccggttacca
      421 aaagctttct aatggaaaga tttcttccat aaaagggcgc tcagtggtca cagtaaacta
      481 ctatatgaca accatagctg gaatgtttca atttgcaaca gataatggtt atacctcagg
      541 aaacccattt aacggtctgg cccccttaaa aaagtccaag gtaaaaccag atcctctcac
      601 ccgtgacgaa tttattcgtt ttattgaggc ttgccgtcat caacaaacaa aaaacctgtg
      661 gattctcgct gtatacacgg gtattcgtca cggggagctg gtatcgctgg catgggaaga
      721 tatagatctt aaagcaagga ctataaccat ccgtaggaat tatacaaaac ttggcgaatt
      781 cactccacca aaaaccgatg ctggcaccgg aaggacaatt catctggttc aaccaactat
      841 tgatgctctt aaaagtcagg cggaaatgac catgcttgga aagcaacatt ctgtagaggt
      901 aaagcagagg gaatatggga gaagtactgt gcataaatgc acttttgttt ttagtcctca
      961 ggtaataaaa cagcggcagt tttccggacc gcactataag gttgactcca tcagggagtc
     1021 atggacaagt atcttaaaac gcgcaggtct gagacacaga aaatcgtacc aatccaggca
     1081 tacttatgca tgctggtcac ttgccgctgg agctaatcct agttttatcg caagccagat
     1141 gggccacaca aacgcacaaa tggtattcaa tgtttacgga gcatggatga aagacaacaa
     1201 tcacgaacag atagaactcc ttaacaaaag actatctgaa agtgtcccat gtatgcccca
     1261 taagaaagct gggtaaaata aaaacttgca aaatcaatta gtttaccctt aatccctgtc
     1321 acgttacgcg cgtggcagag gcgttacggg ttgctgaaac cgcaacggac agacggcggt
     1381 catcgactgt tcaacgatgc cgatattgac cgtatccgcg agatcaaacg ctggatcgac
     1441 aacggcgtgc aggtcagcaa agttaaaatg ctgctcagta atgaaaatgt tgatgtgcag
     1501 aacggctggc gcgatcagca agaaacatta ctgacctacc tgcaaagcgg caatctgcat
     1561 agcctgcgaa cgtggatcaa agagcgcggt caggattacc ccgcccagac actcaccaca
     1621 catctgttta ttcctctgcg ccgacgactt caatgccaac aaccgactct ccaggcgctg
     1681 ctggcgatcc tcgacggcgt actgatcaac tacatcgcca tttgtctggc ttcggcacgt
     1741 aaaaaacagg gtaaagatgc gctggtggtt ggctggaata ttcaggatac cacccgtctg
     1801 tggctggagg gctggattgc cagtcaacaa ggatggcgca ttgatgtcct cgcccactcg
     1861 ctcaatcaac tacgccctga actattcgaa ggccgtacat tgctggtgtg gtgcggtgaa
     1921 aatcgaacct ccgcccaaca gcagcaactc accagttggc aagaacaagg ccatgatatt
     1981 ttcccactcg gcatttaatg attcgttaac aaatgcgctt tactgtacaa tcctttcgtt
     2041 aacataagga gtgcattatg cgcatagcta aaattggggt catcgccctg ttcctgttta
     2101 tggcgttcgg cggaattggt ggcgtcatgc tcgcaggtta tacctttatt ttgcgtgctg
     2161 gctaagcgcc tgcaccagcc tttcaaacag gcggtctgcg atgatcgccg ccagtgccac
     2221 cagtaacgcc ccctggatca catacgcggt attaaatccg ctaagcccga tgatgatggg
     2281 cgtacccagc gtgctggctc ctaccgttga ggcgatcgtc gccgtaccaa tgttgataat
     2341 caccgaagtt cgcacgcccg ccagaatcac cggagccgcc agcggtagct cgaccttacg
     2401 cagtcgctga ccacgactca ttcccatacc tttcgcaact tctgtcacgc tggcatcgat
     2461 cgctcccagc ccggcaagtg tcgcctgcag gacgggcagc acaccgtaaa ggatcaaggc
     2521 gataatcgct ggttgcagac caaagccgat caccggaacg gcaatcgcca gcactgcgac
     2581 aggcggaaaa gtctgtccaa cggcggcaat agtttccacc agtgggcgaa attccgcgcc
     2641 ccacgggcga gtgacagcaa ttccggcacc agtgccaatg atcaccgcaa acaaactcga
     2701 aattcccacc agccagaaat gagccagtgc cagagctgca aaactttctt gctgataaac
     2761 gggtcgtggc agttgtggga acaaggcagc aaacagcggc tggctgtaag gcagccagaa
     2821 aatcagtgcc acaaacaaag caatgagcca gaacagcgga tcgcgcaaca tcttcatacg
     2881 cttacgcctc caccagcaga tcctgaaaat gcagcgtacc gcaaggctgg ccctgcgtgt
     2941 tcaccaccgg cagcacctcg caaccccgcg cgacaaacag ggagagcgca tcgcgtagcg
     3001 tcatctcttc taccagtgcc tcaccttctg cccgttcttc gcgacgcacg taatccgcca
     3061 cactacgtaa cgaaagcaga cgcacaccca gttcgctacg tccaaaaaac tggcggacaa
     3121 aatcattcgc cggacgagtc agcatcgtca gcggattgcc ctgctgcacc acttcaccgt
     3181 gatccatcaa taccagatgt tcagccagcc gtagcgcctc atcaatatca tgagtgacca
     3241 gcacaatagt gcgccccagc aaacggtgaa tgcgcgtcat ctcttgttgc aacgcgccgc
     3301 gcgttaccgg gtccagtgcg ccaaaaggtt catccatcag taagacttgc ggatcggcag
     3361 ccagtgcgcg cgccactccc acacgttgct gctgaccacc ggaaagctga tgcggataac
     3421 gctcacgcaa atttgactcc agccccagta gcgtcattaa ttcgtcgata cgatcgtcaa
     3481 tccgtgcccg cgaccatttt tgtaattgcg gcacggtagc gatgttttgc gccacgctcc
     3541 agtgggggaa caggccaata gattgaatgg catagcccat ccggcggcgc aactccagta
     3601 ctggcagcga gcgaatttct tctccggcaa agcggatctc tccgctgtca tgttccacca
     3661 ggcggttaat cattttcaag gtggtggatt tgccggagcc agatgtgcca atcagcaccg
     3721 aaaaactccc ttcctgaaaa ttgagattaa gatcgttaac ggctttttgt gcgccaaaca
     3781 gtttgctgac atggctaaat tcaatcatta cgtttcacct tcagcagtgc gataagcaaa
     3841 tcgaacaggg catcgatcag caccgccaga acaattaccg ggatcacccc cagcaacact
     3901 aaatcaatgg cgctgcttag cagcccctgg aaaaccagcg caccaaaacc gcctgcgccg
     3961 attaacgccg caatcaccgc catacctaca gtttgcacca tcaccacccg caggctgcgc
     4021 agaaataccg gtaacgccag tggtaactga acatgcagga atcgctgcgc cccgctcatg
     4081 cccatcgctc tggcgctctc cagcacatcg cgcgggatct ggttcaaacc gactaccacg
     4141 ccgcgcacca gcggcagcaa ggcatagagc accagcgcaa tcagtgcggg tgtcattccg
     4201 gttcctgcta tgccgagctt ccccagccac ggaaaggccg taaccagcgc ggcaagcggc
     4261 gcaatcaaca ggccaaagag cgccaccgaa ggcacggtct gaatgacatt gagcagagaa
     4321 aaaattgccc cctgccgcgc agtggaaaag tagcaccaga tgcccaacgg cacaccaatc
     4381 actaacgcag gcagcaccgc accaaacagc aacgtcagat gttgtgccag cgcgtcgtca
     4441 aacacatcct gacggttggc gtattctttc attagtgaga gatcgttaag cgtgccggag
     4501 tacagcaacc acagcggaat aatggcaatc tgcatatgca acaaccagcg ccacagcgga
     4561 tgcgtggaga ttcggcggat ggcatcgcta caggccagca atgccagcgc cgcagccagc
     4621 cagaaaccac tgccgaggct ggtacgcgcc agcgcactgc cattttgcgc cagttgggtc
     4681 gccgcctttc cagctcccca caccaacaat acgaagacga attgcgccag aatgagtgca
     4741 caaatgctgc cttttttagc ggggacaaaa caggctgtca gccaggcgca accaacgccc
     4801 accagcatcc agatcgtttg cggccacagt tgccagagat gacgcccttc acccgagact
     4861 aaacgattag gcgcgtagct gataaacggc agcgctgctg cgattgccgt cagcaacagc
     4921 agcagcgcca gaacaggatt aatacgtaga taagtcacgg gaaattactt cgcccaccct
     4981 ttttgtttca ggtagtcggc agccactttt ttggcatcca gtccttccac tgcaatgctg
     5041 gcattcagtt gctgcaatgt ttttgcatcg aggctggcga agactggctg tagccactgt
     5101 gccatttgcg gatactcctt caacaccgat tcacgcacca ctggtgcagg cgcatagata
     5161 ggttgcacgc cttgcggatc gcttaaggtt tgcagcccca gcgccgctac cgggccgtca
     5221 gtgccgtaag ccattgcagc attaacgcca gaggtttgct gggcagcggc tttgatcgtc
     5281 accgccgtgt cgccaccagc cagtgacagc aactgatcct gaccgagctt aaagccatag
     5341 gctttttcaa acgcgggtaa cgcatcggcg cgttcgataa actctgccga ggctgccagt
     5401 ttgaaggtgc cgccctcttg cagataacga ctcaggtcgg caagcgaagt tagtttattt
     5461 ttctctgcca catcctgacg cacggcgata gtccaggtgt tatttgcagg cgcgggcgtc
     5521 agccagatta acttgttgtg ctccgaatcg agctttttga ctttctcgta accttgctgc
     5581 gcgtttttcc acgctgcatc gttttcatct ttaaagaaga aagcgccatt gccggtatat
     5641 tccggataga tatccagttc accggaagta atcgcctcgc gcaccacagg agtcgttccg
     5701 agttgcactt tattcaccgt cggtacgccg tggctttcca gtacctgcaa aatgatattg
     5761 ccgagtagcg caccttcggt atcgattttt gaaccgactt taacggggga agccgcctgt
     5821 agcggcaggc tcacggctgc caacataacc agtgaacctg cccagagctt taagagtggc
     5881 atgatgcttt cctcattctt ttactgttgt tttcagcgaa ttaagagaaa agcatagttg
     5941 ataatggcgg ggttagcttg agcggaagga ttcagttgca gaatcagata aatacttaag
     6001 aggcatattc ggtccggcgt ttcctgccgg atgcggcgcg agcgccttat ccggcctaca
     6061 aagggcgcaa acgtcgcgcc cttactaaag cattacagca actcaaactc gcctttctta
     6121 acgcgtgcgg aatcagtgcc gataaataca ttgaacttgc caggctcggc gtcatatttc
     6181 atctgttgat tccagaactt cagcgcctca atatcgatcg ggaagctgac agtctgagtt
     6241 tcgcccggtt tcagggtgat tttctcaaag cctttcagct gtttcacagg gcgactcatg
     6301 gaagccgtca catcctgcaa gtacatctgc actaccgtgg ccccctcgcg cttaccggtg
     6361 ttcgtcacct gcacgctggc agtcactttg ccgtcacgct tcatggtcgg cgcagaaagt
     6421 ttcacatcag agacggtgaa agtggtgtag ctcagcccat agccgaacgg atacaacgcc
     6481 ccgttagctt catcaaaata acgcgaagtg tatttgttcg gcttgtcggc attatacggg
     6541 cgaccggtat tcagatggct gtagtacacc gggatctgcc cgacagaacg cgggaaggac
     6601 attggcagct tgccggacgg gttgtaatcg ccaaacaata catcggcaat tgcattaccg
     6661 ccttcagtcc ccgcaaacca ggtttccaga atcgcatcag cctgctgatc ttctttcacc
     6721 agcgccagcg gacgcccgtt catcagcacc agcaccagcg gtttaccggt ggctttcagc
     6781 gccgcaatca agtcacgttg gctttgcgga atagtgatat cggtccggct ggaggcttcg
     6841 tgcgccatcc cctgtgcttc accgactaca gccaccacca catcagattg tttcgccgtc
     6901 tgcaccgctt catcaatcat ctcttgcggc gaacgcggat cgactttgac cgcttcttca
     6961 tactgattca ggaaatcgat aatgcctttg tcactggtaa cgttcgcccc tttggcatac
     7021 agcactttac cgttttcacc gacggaattt ttaatcccgg tcagtacggt cacggattga
     7081 tcggcaacac cggctgcgga ccagctgccc atcacgtcac gtttactgtc cgccagtggc
     7141 ccaaccaccg caatggtggc cgattttttc agcggtaacg tttcgagacg gtttttcagc
     7201 aacaccaggc tttcgcgcgc cacttcacgc gcttctttac ggtgcaggcg gctttcggca
     7261 ttggtatcca ccgggtcaga ctctttcggc cccaaatggc tgtatgggtc gttaaacaat
     7321 cccatatcat atttaacgtt cagtacatgg cgggcagcat cgtccagctc ttccatcgtc
     7381 actttgccgg atttgatcaa cccaggcaga tacttcgagt aatactcgtc gctcatactc
     7441 atgttgattc cggatttcag cgccacgcgc accgcatctt ccgggtctgc cgccgtgcca
     7501 tgtttaatca gctctttgat tgcaccgtga tcggaaacgg tgatgccttt aaagccccac
     7561 tggtcgcgca gaacatcttt cagcagccag gaatcggagg tggctggcgt gccgttcagc
     7621 gagttcagcg ccaccatcac cgcgccgctg cctgcgtcca gccccgcttt gtacggcggc
     7681 atataatcat taaacaggcg ctgcggactc atatcgacgg tgttgtactc tttaccgcct
     7741 tctaccgcgc cgtatgcggc aaagtgtttg acgctggtca tcaccgagta gcgatctgcc
     7801 gggcttttac cctgcatcgc ttccaccatg gttttaccca ttgttgaggt gagatacgta
     7861 tcttcgccaa aaccttcgga agcacgtccc cagcgcggat cgcgcgagac atcgaccatc
     7921 ggtgcccagg tcatattcag gccatcatct gccgcttcat aagcagagac acgtccgacc
     7981 gttttcactg catcgaggtt aaaagacgag gccagaccga ggctaatcgg gaacaccgtg
     8041 cgctgaccgt gcagcacgtc gtaagcaaag aaaagaggaa ttttcaggcg gcttaattcc
     8101 atcacctgat cctgcatggc gcggatatcc tgacgggtta cggtgttgaa aatcgcccca
     8161 acctgaccgt ctttgatcat ctcgcggatc gcctctttcg ggttatccgg gccgacgctg
     8221 attaagcgca gctgaccaat tttctcatca actgtcattt tcttaagcag ttcggtgacg
     8281 aacgcatccc gcgcttcggg cgttaatgga tggttgccga ataaatcatc cgccagtgct
     8341 ggctgcaggg ccagactcac cgcgattcct actgaacata gccatttcat atggattttt
     8401 cttcctctgt tgcccgacga ggcagcgaaa atggtgcaaa aaccgtagtt tgccataagc
     8461 atgatggaga gagaaaaaga atgctcagtt tattgtctga attttcaaaa tattcactcg
     8521 ctgaattgtc atacaaggcg ctattctagt ttgtgatatt ttttcgccac cacaaggagt
     8581 ggaaaatgtc ttccatgaca acaactgata ataaagcctt tttgaatgaa cttgcccgtc
     8641 tggtcggtca ttcacacctg ctcaccgatc ccgcaaaaac ggcccgctat cgcaagggct
     8701 tccgttctgg tcagggcgac gcgctggctg tcgttttccc tggctcacta ctggaattgt
     8761 ggcgggtgct gaaagcctgc gtcaccgccg acaaaattat tctgatgcag gctgccaata
     8821 caggcctgac cgaaggatcg acgccaaacg gtaacgacta tgatcgagat gtcgttatca
     8881 tcagcaccct gcgtctcgac aagctgcacg ttcttggcaa gggcgaacag gtgctagcct
     8941 atccgggcac cacgctctat tcgctggaaa aagccctcaa accgctggga cgcgaaccgc
     9001 actcggtgat tggatcgtcg tgtataggcg catcggttat cggcggtatt tgtaacaact
     9061 ccggcggctc gctggtgcaa cgtggcccgg cgtataccga aatgtcgtta ttcgcgcgta
     9121 taaatgaaga cggcaaactg acgctggtga accatctggg gattgatctg ggcgaaacgc
     9181 cggagcagat ccttagcaag ctggatgatg atcgcatcaa agatgacgat gtgcgtcacg
     9241 atggtcgtca cgcccacgat tatgactatg tccaccgcgt tcgtgatatt gaagccgaca
     9301 cgcccgcacg ttataacgcc gatccggatc ggttatttga atcttctggt tgcgccggga
     9361 agctggccgt ctttgcggta cgtctagata ccttcgaagc ggaaaaaaat cagcaggtgt
     9421 tttatatcgg caccaaccag ccggaagtgc tgaccgaaat ccgccgtcat attctggcta
     9481 acttcgaaaa tctgccggtt gccggggaat atatgcaccg ggatatctac gatattgcgg
     9541 aaaaatacgg caaagacacc ttcctgatga ttgataagtt aggcaccgac aagatgccgt
     9601 tcttctttaa tctcaaggga cgcaccgatg cgatgctgga gaaagtgaaa ttcttccgtc
     9661 cgcattttac tgaccgtgcg atgcaaaaat tcggtcacct gttccccagc catttaccgc
     9721 cgcgcatgaa aaactggcgc gataaatacg agcatcatct gctgttaaaa atggcgggcg
     9781 atggcgtggg cgaagccaaa tcgtggttgg tggattattt caaacaggcc gaaggcgatt
     9841 tctttgtctg tacgccggag gaaggcagca aagcgttttt acaccgtttc gccgctgcgg
     9901 gcgcagcaat tcgttatcag gcggtgcatt ccgatgaagt cgaagacatt ctggcgttgg
     9961 atatcgctct gcggcgtaac gacaccgagt ggtatgagca tttaccgccg gagatcgaca
    10021 gccagctggt gcacaagctc tattacggcc attttatgtg ctatgtcttc catcaggatt
    10081 acatagtgaa aaaaggcgtg gatgtgcatg cgttaaaaga acagatgctg gaactgctac
    10141 agcagcgcgg cgcgcaatac cctgccgagc ataacgtcgg tcatttgtat aaagcaccgg
    10201 agacgttgca gaagttctat cgcgagaacg atccgaccaa cagcatgaat ccgggaatcg
    10261 gtaaaaccag taaacggaaa aactggcagg aagtggagta aaaattacgg atggcagagt
    10321 atcgccatcc gaattcactt aatcgttctg tgccgtctgc cccgccgccg ccatttgggc
    10381 ggctttttgt tttttatagc tcaacgctgc tgccggcaca ggcatcactt taccggtttc
    10441 aatccaggta cgcaggcggc tggcatcggc aaaatgggta tatttgccaa acgcgtccat
    10501 cactaccagc gccaccggtt tattattgat aaccgtacgc atcaccagac aatggcccgc
    10561 cgcattggta aagccggttt tggttaactg aatattccag ttatcgcgat acaccagatg
    10621 attagtattg cggaacggca gcgtatacgt cgggttagag aaggttgcca tatcttcccg
    10681 ggtagtactt aactgcccga tcaacggata ctgtttgctg gcaatgagca gtttggttaa
    10741 gtcacgggca gttgaaacgt tatgcaccga caatccggta ggttcaacaa agcgcgtgtt
    10801 gttcattccg agcgatttcg ctttcgcatt cattgcctta ataaaggctt tgtaaccacc
    10861 gggataatgg tgcgcaaggc ttgccgccgc gcggttttct gaagacatca gcgccagcaa
    10921 cagcatatct ttacggctga tttcgctatt cagtcgtacg cgcgaataga cccctttcat
    10981 ctccggcgtc tggctgatat ccacttttag tttttcatcc agcggcagtc gtgcatccag
    11041 cacaaccatc gcggtcatta atttgctgat agacgcaatc ggacgcacca gatccgggtg
    11101 gttcgaatag atcactttgt tggtattcag atcaacaatc atcgcgctac cggaggcaat
    11161 ttccggttgt gaagcggtgg tagcggctgc cgttttcgca acggcctgcg gtgcaaaagg
    11221 cacagccagc atcagggcca ggctaaataa agaaactcga aatttcggca tgatgagcat
    11281 tcagatagtg gttcacgcgc acgggttgcg caccgccgga gtaaggattt actgaggcta
    11341 gcgacgccat cataacgagc aaaaagtgcg atcgtcaaag gagaatcgtg aggaaatgct
    11401 gcattgctga catttacgcc agcaatgcaa cgtcaaagaa ctttctagaa caaacgataa
    11461 ccgtagcccc atagtataac ggttagggcg agcagcactt ccagtaccag cacgccaatc
    11521 gccagcgtcg aactagagaa gctaaggcct tcctctttgt tgatattcag gaagctcgga
    11581 ataccaaggt aaagcaggta gccggtgtaa aacagcgcca ccgtgccgac cagcgcacac
    11641 aaccagacca gtggataaag cgccaccaga ccgcttaaaa acagcggagt tgcaacgtag
    11701 cccgcgaaga ccatgcagtg cgcaagtgac ggacgctgcg gataattacg cgccatccac
    11761 cagatgaccc gccccatcac cgcgacccca gccagcataa cgccgtaaaa caagacagcc
    11821 agcgccagtc cggtaaacca ggataacttc aggatagtgc catcgccaaa attccagcca
    11881 atctgtgtag tgccaatgaa ggcgcaaatc accggaatcg ccgccatcag caaaacgtgg
    11941 tgggtgtaat ggtgagaaat cgtttcgttt tcgcgattaa tcacctgcat ttcacgatcg
    12001 ggatgggaaa acagtcccca gacatggctc ataccgcccc cttgttgtga gttcatgaac
    12061 ctgacagttc aagtataagt cagcttgtga ttattttttg ttcgcccatg taattttcac
    12121 tgtctgatga acgtcctttt tcagcgtgaa tgattcacag ggtgtatgct tactggcaac
    12181 caaagggaga cagactggcc tatggatctc aatacactta tctcacaata tggttatgcc
    12241 gcgctggtga tcggtagcct ggcggaaggt gaaaccgtga ctttgctggg aggcgttgcg
    12301 gcgcatcagg ggctattaaa gttcccgctg gtggtacttt ctgtggcgct tggcggcatg
    12361 attggcgacc aggtgctcta tctgtgcggg cggcggtttg gcggcaagct gttacgccgt
    12421 ttctcgaaac atcaggataa aattgagcgg gcgcagaaac ttatccaacg ccatccgtat
    12481 ctgtttgtca ttggtacgcg ctttatgtat ggctttcggg tgattggccc gacgctgatt
    12541 ggtgccagcc agctgccgcc gaaaatcttt ctgccgctga atattctcgg cgcatttgcc
    12601 tgggcgttga tttttaccac tattggttac gctggtggtc aggtgattgc gccgtggttg
    12661 cacactctcg accagcattt gaagcactgg gtctggttga ttctggtcgt ggttctggtg
    12721 gtaggcgtgc gctggtggct gaaacgacgc gggaagaaaa agccggataa tcaggcgtaa
    12781 aaccattgcc ctggataagg cgttcgcgcc gcatccgaca tccggggcac catgcctgat
    12841 gcgacgcttg tgcgtcttat catgcctacc aaaattttac ccattgaaat aaccacgcac
    12901 ctcactgcgc tattctgggt tgaactgcgg attcgccaac ataaagccgc catccactat
    12961 caacgactgc ccggtggtgt aatttgcgcc ctctgagcac agccacgcta ccaggctggc
    13021 aatctcatgc gttgcgccaa aacgccgcag aggaatcgaa ggctccgcgt cgggcttcac
    13081 gtagctgtca tccatgccat tcattggcgt ggcgatcgcc ccaggcgcga ctgcgttcac
    13141 caaaatctta tgcctgacca gctccagcgc catcgttttg gttaacccgc cgagcgcatg
    13201 tttagcggct gtgtaagcgc tggcatccgg cagcggcgta tgttcatgta ccgacgtaat
    13261 gttgatgatg cgaccgccct gcccttgttt caccatctgg cgggccgcaa tttgtgagca
    13321 taaaaatgca ccatcgacat caacggtaaa aatcttgcgc cactcatcga aagccatatc
    13381 aagaaacggc gctttggtca ttgcgcccgc attattcacc agcacatcaa tacgccccag
    13441 ccgttgaatg agtttctcca gcgcctgtgc accttctggc agtttaccga gatccagctg
    13501 cacaatctcc gcacgtacgc cgtggctaac tacctcacgc gcggtatctt ttgccccttc
    13561 ttcatctgag tgccaggtaa tgccaatatc aaacccctgc tgcgccagta ataacgcgca
    13621 ctctttgccg atccccgaat cggaggcggt aataatcgca acctgtgcca tcgagttctc
    13681 cacttaacgc tgaataaacg ttaagtatag aaggcgcata tcatcagcgt ttgtaacccc
    13741 cgcccaacgc accagtgagt tgaatggaag catccagcca ctgcccttgc aataacaggc
    13801 cattggcccg ctcacgcagc gcggggattc tggcttcgct gacgcgggaa ccagcaatga
    13861 tgcccgcgtt aaagcgcgcc tgcgcaagac ctaccacacg caaggcatcg cgctcaattt
    13921 gcgcctgatg ctggtttttc tccgcaagtg tctgaacctg actggctgcc cgcgccacgt
    13981 cattcaccgc ttcaaccacc gctttgttgt agctggcgat agacaagttg ctttcggctt
    14041 ttgcgatatc gagattggcg ttaagacgac cactatcgaa aatgggtagc gtcaggcctg
    14101 ccgtaacgcc catttgctgc gcggaatgac ggaacagatc gcttaagtgc aacgcatcct
    14161 gttgcaggaa ggccatcagg ttgatgtcag gataaaatgc cgcttttgcc gcatcaatgg
    14221 tgcttagcga tgactcaacg taccagtgcg ccgcctgcaa atctgcccgc cgggccagta
    14281 aggagtaccc cagttcatca ggaagctggc ttgccacttt cggcaacgcg accggtttaa
    14341 gcttcaatga ctttgtctgg ttatttgtaa gtgcgcttaa ccgtgcctca ataattttca
    14401 ttttccccgc gacatcgttg agctgctgcc gggttttgct ggcattaata tcggtttcca
    14461 caccttcaac tgaagaagta atcccgttct gatatagctg gcgatcggtc gcgataatgg
    14521 tgttctgctc tttttctatt tgctgcaaga ccgtgtttaa cgccgcctgg gtttgccact
    14581 cccagtacag gcgggctacg ctgccagcca gcaattggcg ggtttgctcg cgttccgccg
    14641 cccgtgcttt aaccgtaccc aggcgggcag taacctccgc ccgattcttt ccccagatat
    14701 cgagatgcca gcccgccgtt aagccaaaag ttccgttggt gtaccacgga ccggtcgtac
    14761 ctgcggccgg atcgttcaga gcaaacggcc ccattaagcc ttctgccgac attttttgcc
    14821 gctccatatc cgcagaaaag tcgatctgcg gaccatcctg agtagcaatt gccttcgcct
    14881 gggcttcagc tagctgaatg cgctgttcag ccacctgcat atccggtgcg ttctgtagtg
    14941 cattgttaat taaggaagtg agttgattat cgtgatactc cagccaccat tggctgtctg
    15001 gccaaccatt tttcagcgcc gtgggtaatg cggtgtcaac ttgtgcagcg ggcgtttgct
    15061 ggcttaacgc ctggcgggtt tcatgcatag gcgcacaccc ggccagcatc agtaacagcg
    15121 gaaaacaggc gatggcggga taaaaggaat cacgattcat gggggaataa tcaggtaaga
    15181 aaaggtgcgc ggagattacc gtgtgttgcg atatattttt tagtttcgcg tggcaataca
    15241 tcagtagcaa taaaacgaca tatccagaaa aatatacact aagtgaatga tatcttccga
    15301 tttatcttaa tcgtttatgg ataacggcaa agggcttcgt tttttcctat acttattcag
    15361 cactcacaaa taaaggaacg ccaatgaaaa ttatactctg ggctgtattg attattttcc
    15421 tgattgggct actggtggtg actggcgtat ttaagatgat attttaaaat taattaatgt
    15481 catcaggtcc gaaaataacg agaatatttc agtctctcat cctgttgcgc tcctgtcatg
    15541 tgtattgctt catataatca ctggcgca
//
LOCUS       DAZFQD010000125        15564 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.125, whole genome shotgun sequence.
ACCESSION   DAZFQD010000125 DAZFQD010000000
VERSION     DAZFQD010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15564
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.125"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(5..3127)
                     /gene="agn43"
                     /locus_tag="ACHKTW_003945"
                     /gene_synonym="flu"
                     /pseudo
     CDS             complement(5..3127)
                     /gene="agn43"
                     /locus_tag="ACHKTW_003945"
                     /gene_synonym="flu"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026164.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin Ag43"
     gene            complement(3461..4333)
                     /locus_tag="ACHKTW_003946"
     CDS             complement(3461..4333)
                     /locus_tag="ACHKTW_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HFO6464873.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLTSHEPVIGIMGK
                     SGAGKSSLCNALFQGEVTPVSDVHAGTREVQRFRLSGHGHSMVITDLPGVGESRDRDA
                     EYEALYRDILPELDLVLWLIKADDRALSVDEYFWRHILLCGHQQVLFVVTQADKTEPC
                     HEWDMAGIQPSPAQEQNIREKTEAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALP
                     DHAASPLMTRLQDELRTESVRSQAREQFTGAVDRIFDTTESVCVASVVRTALRAVRDT
                     VVSVARAVWNWIFF"
     gene            5674..7164
                     /locus_tag="ACHKTW_003947"
     CDS             5674..7164
                     /locus_tag="ACHKTW_003947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfjI family protein"
                     /protein_id="HFO6464874.1"
                     /translation="MPYFQYPDEFPLSSLPPLIRDAVIEAQQITQAPLGLVAASALGA
                     VSLVCQNLIDVCRLNTLRGPVSLFLLTLAESGERKTAVDKLLMEPLYQQEMLLYSRHK
                     NELTSWKNKEEILKAQKKALLSKLNKELRKGADESETLRQLEALQKNREEKPVRYKFI
                     FNDATTAAIKDQLCGQWRSVGIMSDEAGIIFDGYTLSELPFINKMWDGSVLSVDRKNE
                     PEQMIENARMTLSLMVQPGLFDRYMERKGSVARDSGFLARCLISKPATTQGKRFINGA
                     VTPGGSLTAFHERLMELARGSIEKSSKDERYCLHFSPEAQKIFIDHYNVLEQDLSPSG
                     PLSQFRGHVSKKTENIARIAALLQYFSQGEGKISADIMTSAVVISSWYTDEYKKLFAL
                     PDESELQLQDARELLDWLIEECRGECPPRVRKNYILQCGPGRFRNRKKLNVLLNILAS
                     QLRLSVVQEGKTTYVLLSEINNITLDKLNACYSQKLIRWHEQSFGL"
     gene            complement(7286..7858)
                     /locus_tag="ACHKTW_003948"
     CDS             complement(7286..7858)
                     /locus_tag="ACHKTW_003948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6464875.1"
                     /translation="MQKLENRCELLLIQHQKWMTSVTRLIVAHGMGSPHLHGYHRLTL
                     AHFFLPEKGSIISVAPQGLYQVVNPGTPPFIPAIQEGLMTSIQTHEIMLLTHFNLGGV
                     LLSELHRLGENRLANRLNSLLRRFDDRDLYHTLIWLCWYDLMCAHSMQPWTEELKHKS
                     HAELENWAVARKREKRELELMIDEYLLYAC"
     gene            complement(7944..8150)
                     /locus_tag="ACHKTW_003949"
     CDS             complement(7944..8150)
                     /locus_tag="ACHKTW_003949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO6464876.1"
                     /translation="MNTPVSLMDDQMVDMAFITQLTGLTDKWFYKLIKDGAFPAPIKL
                     GRSSRWLKSEVETWLQARIAQSRP"
     gene            9007..9231
                     /gene="hha"
                     /locus_tag="ACHKTW_003950"
     CDS             9007..9231
                     /gene="hha"
                     /locus_tag="ACHKTW_003950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004152106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HFO6464877.1"
                     /translation="MQHKVTRDEWKLKPEQFRRCSSRETLEKVSSHTRYKLTPAELEA
                     FNSAVDHRLAELIMNKLYDRVPASVWKYVI"
     gene            9866..10435
                     /locus_tag="ACHKTW_003951"
     CDS             9866..10435
                     /locus_tag="ACHKTW_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HFO6464878.1"
                     /translation="MTYKYNPFWQQRIRETVLRALDVHPRLTALRVDLRLPDVPAATD
                     AAVISRFINALKARIDAYQKRKRREGKRVHPTILHYAWAREFGELKGKKHYHLLLLVN
                     RDTWCRAGDYRAPGSLAGMIKQAWCSALGVDAGCYATLANFPAWPVVWLERGDDTGFQ
                     QVLERADYLAKESTKAYGTGERNFGCSRG"
     gene            10602..10985
                     /locus_tag="ACHKTW_003952"
     CDS             10602..10985
                     /locus_tag="ACHKTW_003952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HFO6464879.1"
                     /translation="MYAKSFLALDGNGRLTGARTAQTAPYDRYTCHLCGSALRYHPQY
                     DTERPWFEHTDDGLTEHAQQCPYVRPDRREVQLVKRLRQFVPDALPVVRKASWYCRQC
                     HHDYYGERYCTHCQTGRFSEEGGAE"
     gene            10982..11407
                     /locus_tag="ACHKTW_003953"
     CDS             10982..11407
                     /locus_tag="ACHKTW_003953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HFO6464880.1"
                     /translation="MNGIKTGLGITPGEHIISADSALSRNIRHCFCLSCHGRLILQTD
                     AQGAWFEHDLHALSEQQKADCVVLNPEKSHPYIEDMAMFLSPLPVVLEWHCVMCGRLF
                     PGKKFCEACGTGIYCRAVCTKSVYSYQPDLFEDCGSAST"
     gene            12943..14067
                     /locus_tag="ACHKTW_003954"
     CDS             12943..14067
                     /locus_tag="ACHKTW_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide disphospho-sugar-binding
                     domain-containing protein"
                     /protein_id="HFO6464881.1"
                     /translation="MRKRILFIGPPLYGLLYPLISLAQAFRVIGHDVVISSAGKFANK
                     AAEAGLVVFDAVPGLDSEAGYRHQEELRKKSNIIGHFSFFSDEMADNLIDFAGKWRPD
                     LIVYPPLGPAGPLVAAKYRIPSVMLAVGFAHTSAHIQMLNRSLSNAYRRHGVSGPLCD
                     LAWIDVAPPSMSILKNAGEPVISMRYIPYNGGAVKETWWDRDSDRKRLLISLGTVKPM
                     VDGLELISWVMDSANEVDADIILQLAINARTGLRKLPSNVRLVDWIPMGVFLNGADGF
                     IHHGGAGNTLTALYSGIPQIVFGEGADRSVNAEIVAKRGCGIIPDKHGLTSDLVNRLL
                     YDDSLRFCSDQVAAEMAEQPSPAEIAEVLMRKLKNNGKQL"
     gene            complement(14209..15483)
                     /locus_tag="ACHKTW_003955"
     CDS             complement(14209..15483)
                     /locus_tag="ACHKTW_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001390635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HFO6464882.1"
                     /translation="MIPMKNYLFQTPEDICVQLKKMTHPVTIRTTDIANFWHYLESAT
                     LPVITKSTTTENREVTFLWRSEKALQGVYLRLNRVTDKKDVKKGLMTHIPSTDIWMLT
                     LVLPASYRGSYSFIEIPTDMTQKDIFQLGSRFSPLPGKSDPFNKTAEINIRGFGESVL
                     SLDMAPEQKEWDDTSHKCTGILSTLHSFVAGYQRRIRLYFPLNPTSVPLGLLVLPDAE
                     TWFDRIDITRALDMAITTGRITPMAIMGIDNINESDRMNILGGNKELIFDIAENLIPQ
                     LYRDYPDIVWAGRSNTVLAGQSLGGVTALMAAIYASTTFGTIISHSPSMWWNPDQGSP
                     ILFTENNTSWVSEQILSAPPKDVNIRLGVGSLEGTTVSHVQRLHHSLIAAGLESNLTV
                     YTGGHDYAWWRGAIIDELANYNCRKVSNNNSV"
BASE COUNT         3791 a         3720 c         3993 g         4060 t
ORIGIN      
        1 attatcagaa ggtcacattc agcgtggcct gactgttata accttcagcg ctgctgccgt
       61 tgatgctgtg ggcataaccg gcctgaacgc ccagggtgat attttcccgg acacgggctt
      121 ccagtccggc ctgcagatcc agtgaagtgc cattctgtga gggagagaac gtcatcccgc
      181 tgcctgcggt ggaggtcccc acacgcatat caccccggga gctgaaggtg cggataacgg
      241 aaggctgtac ccactagttc accggcagtt cacgcacact gtgttttgca ctgtcacgca
      301 ggggggcacg ggatgaggtg ccttcaccaa aggtcatatc gttgtggctg cccagacgga
      361 aaccggcacg cacatgctgt gcactgccat gcccgaactt cacataaccg gcgttgtcct
      421 taccgtcatc cagcgaaagc ccctgccagg tgtactgcag ccgtggctcc agcatcaggt
      481 tgtcagtgat actgaagggc agaccggttt ccagtgagcc cagccacccc cagccccgtg
      541 cgcggaagtc gttattgccc gatgacgctt tcatactgtg gcgggttccc tgtgccacaa
      601 tgtcagccca caggccggag gaggtgtgtg tcaggttcat atatccgccc aggctgccgg
      661 catcatcccg gaccgtgccg gcgcgggagc cgtcatcatc cttaacatca acggaagaat
      721 ggcctgcagc accatacact ccagtcgtca gtgacatacc ggcgacctct gttctgagca
      781 ggtcaccctc cagacggacg aagccatagc tgccgctgct ttccggcgtg gccccacggg
      841 caataccgcc gttgttatcg tgaccgagat ggccgccctg aatgctgaga cggacgctgt
      901 tattttcacc gtttacaccg gtctgatggc tgcgggagcc tgccagaatc cggtcatagt
      961 ccattgcctg tgtcagcatg gaggcgtaca gggggacttc agcacgataa cgttcttcac
     1021 tgcgcagata ccagctctca tcactgtccc ggttgagggt gtagttaaag gccccggcct
     1081 gcagcatatt ccccaggaca aaagcccctt cctccgtggt ggcaccgtta atggcctcaa
     1141 ccacctgaat acccttaccg gtggtcgcca gccccgtacc actgttgccg gcgttcacca
     1201 gattcaggat ggtttttccg gttgccctgc caccgtcaat gaccagtctg tcagcattgt
     1261 tctgcgccat atccgggcgt acacgcaggc tgatggtgcc attctgcccg ttcaggtttt
     1321 tcacctgcag ggttgtcgga acgaacttcc ctgtgcgggc ggaggtgaaa tgaatctgtc
     1381 cggcatggct gaggtcatcc accaccgact gaaccgtggc gttatcgggg atattccagg
     1441 tggcaccgga tgtcagggtg acattcgtgg ggtcaatggc accgttcagc actgtgctgc
     1501 cggtcagctt cagggccgtg ccgcgctgag cgatgacatc cgtggtgacg gtactgtcgt
     1561 tcagcgtcag cgtgccttca ttcaggttga cggttttctg ggtgagtgtg gtgttgctga
     1621 cagtgagtgt gccacttccg gatttttcca ccctgccgtt accggtaaga gcgcctccct
     1681 gcaggagtgc atcaccttca cggatggtca gggtatcgtt attcaccgtt ttcccggaaa
     1741 gggtaagcgt ggcaccgtta ttcagcgtgg tggtgcccgc cagcgagccc cctctggcgg
     1801 tgaacagtcc gccatttacc gtggtatccg tggccgtatc accggcgttc agtgtgaagg
     1861 aactgccttt ttccagcatc agggcatccg cctgaccgcc tccgatacgg aatgccgttc
     1921 cgtcgctccg ggtaccactg acagcggcac cggaatcggc cagcagtacc ccgccattcc
     1981 ccatggatac ggtggtggcg gcaccaccgt tgcggacatc cagcgttccg ccatcatcca
     2041 cacgggtatt agtggctgtg tgtccgctca gcacatccag acgtccgcca ttttccagta
     2101 cgacattatc agccttaccc gccacaacgg agaatgctcc caggcggttt gtaccggtga
     2161 cggttgcagc tgtactggta accagtgcac cgcccgtgtt ctgggtgaca tcagacgctt
     2221 ccccgccggc attcacctgc agctttcctt tctggttgat ggtggtatgc gcggcagttc
     2281 ctccttcctt aatcacctgc cagcccccct cgttaattac cgtgttcagc gcaagtccgt
     2341 atttgtgcac atactggttt cctccctcca gccgggtgtt atgtgcctct ccgtgtaccg
     2401 tctggtcacc gccggcgtaa atgagggtgt cccgtgccat ccccgaagac cagataacct
     2461 gccggccatt tttgttgatg gtggtggatt cagccgtccc tccgaccatc tgaccgctgg
     2521 acacattttc agactccgga ccaccttctg caccggtgtt gacgatggtt ccggttgcca
     2581 gtccgccatg ttttatggtc tggtaaccat cctggttaat aattgtaccg gcagctttcc
     2641 ctcccccgtg tacccactgc tcacctctgt tatccagcgt ggtgttcacc gccagtccgt
     2701 taaggctctg ccctccgcca tcacgaataa ccgtatcact ggcagttcct cctgcctgca
     2761 caatctgacg accatttgcg gtgacagtgg tgtttctgcc tgtgccaccc gcttttatcc
     2821 attgcccgcc ggtgttttcg tcactgtccg gccccagctc aagcccggta ctgacagtca
     2881 cgccatcagc tgttccggat acaaactggt tgtcatggtt taccagtgtt ccgccattca
     2941 ctgtttcacc cgggtgcaca acgatgtcag cagccagcac cgggagtgac gtgaccgcgg
     3001 caagagacag tgcaaccgcc acaccggtgc gtttcccccg tgcgcgggcc agttcggagg
     3061 caaccacgaa agcgcccgta atgtgattcc ataccagcct gtagcaggta ttcagatgtc
     3121 gtttcatcag catttcctta atcagagtga gggtgaataa aaaagccggt atccacagat
     3181 gtggataccg gcaggtaaag ggttacagtg ccggaggtcc ggctgacggc agtttctgac
     3241 gtgtggctct gttgttccct gaatctcatg tctgtgtgta tctgcgcacg ttgctaacag
     3301 gagttattat tagcataacg atcgatatta acgatcggtg aaataggtat cgatcgtttt
     3361 attctattat ccggctattg cacggacaag agagacagta acgaaaacag cgcttttgtg
     3421 agcagactca gaggcaggga ggagatcatc cacaacaggt tcagaagaag atccagttcc
     3481 ataccgcgcg ggcaacagag accacggtgt cacgaacagc gcgcagggcc gtacggacaa
     3541 cagaggcaac acagacgctc tccgttgtgt caaatatccg gtccaccgca ccggtaaact
     3601 gttcacgggc ctgcgagcgg acagactccg tgcgcagctc gtcctgcagg cgggtcatca
     3661 ggggactggc ggcatggtcg ggaagtgctg tcatgagcgc actgaccagc gtatccagtt
     3721 cccagccggt gcgggccgat acggccacaa ccggatgtac gggccggaac agccggaata
     3781 ccgcctctgt tttctcacga atgttctgtt cctgtgcggg agaaggctga ataccggcca
     3841 tatcccattc atggcagggt tccgttttgt cggcctgcgt caccacaaac agtacctgct
     3901 gatgtccgca tagcaggatg tgtcgccaga aatactcatc cacagacagg gcacggtcat
     3961 cggctttaat cagccacagt accaggtcca gttcaggcag aatgtcacgg tacagggctt
     4021 catactctgc atccctgtcc cggctctcgc ccaccccggg caggtcagtg ataaccatgc
     4081 tgtgaccatg gccactcaga cggaagcgct gcacttcccg ggtgccggcg tgaacatcac
     4141 tgaccggtgt gacctccccc tgaaacaggg cattacagag tgaggattta ccggccccgc
     4201 ttttacccat aatgccaatc acgggttcgt gactggtgag tttacgcaga tgttccagga
     4261 tgtgacgtga aagcgagtaa ggcagggagg aaagcggttt ttcaattgcc tcaatggcat
     4321 cagacggatt catacatgta ttcccggtga aaaaagacaa aaccccgcgc accggaaagg
     4381 tggcggggga tccacggaga taatatctgt ctgaaattgt tttgaatcga tgggattgtt
     4441 gcgtattaac atgaaggaga tcaggactta tccgttactg ggtgatctcc ttccatgaaa
     4501 atacgcggtt attgttaaac agcaggctga ttactccgta aaggagaaaa atattcatct
     4561 tgcagcctga ttatcaggtg tgtttatgtt tactgaggtg agtatttttc agtatattgt
     4621 cggggcagtc agtgggagat attaaattta cgtttagagt tatcttgctt ctgctgtgta
     4681 tttttataag agtgcgcacg taatgatatc tgttgatacc atgttgttaa gtgtttccct
     4741 tccgggtcat gcgtacttgc aaaatggcat ctgaatcgat cacggtttgg ccaaaccgtg
     4801 atcgaataga gaggaatacg catgtaatga ttaaatgctt ttgttacatc attcttccgg
     4861 catgaatatt tatccagttc ccttttttat ttgtgtagtg atactgtcgg aatagcgttc
     4921 agtaatgtaa agtgtcctgt taaaaatctt taataaatgt gtcaccctgg tcaggaaata
     4981 tctgctgaga tattattagt tggtggtgtt tttactgagt aatagctggc tttttattcc
     5041 tgaactgaaa tactgtcgct cgtcagggtt taattaaaac tggatgatga tgtgaaaatg
     5101 aaggctggtt tttgtgtttg tcgaggaagt gttgttttta tccattctca tcattggctg
     5161 gctgcgatta tacagaagta tttgaagaaa aaatggtgca ttttatctgt gccggcaggg
     5221 agggggattt tcctgatatg tcagtattaa atggtttttg ttatttataa aaatgtcatc
     5281 ctgtccatag aatattgtac tgagtactca gcattcagcg caggaactgt catcttcttt
     5341 attttattct ccttcaggag ggcagttttc tgaaagcgtt actccgggga aactgtttat
     5401 gtttattccc ggcaggaaac ccgtacctgc aataccctgg atgatgcttt ttgctggtac
     5461 gttgttgtgt gaaaattaat caacatgtgt gctccggaga gcgcccgtag acgcacagat
     5521 tcattctgac attctttagc gacaaaatac cgagtggtta ttatttaacc tgaaaatcga
     5581 cagaaatttt tatcctgtat tgattctgct gctatttttg ctattagtag caatagttgc
     5641 ggtactttat gttctgtgtg ttgtgaggtc attatgccat actttcagta tcctgatgag
     5701 tttcccctga gctcgctgcc tccactcatt cgtgatgccg ttattgaagc tcagcaaatc
     5761 acgcaggctc ccctggggct tgtggcagca tctgccctgg gcgctgtttc acttgtctgc
     5821 cagaatctga ttgatgtctg cagactgaat acactacggg ggccggtttc tcttttctta
     5881 ctgacgcttg ctgagtccgg agaacgaaaa accgcagtgg acaaattatt aatggagccc
     5941 ctgtaccagc aggagatgct gttatattcc agacacaaaa atgaactgac gtcatggaaa
     6001 aataaggaag agattttaaa ggcgcagaaa aaagcattgt tgtcaaaact gaataaagaa
     6061 ctccgcaaag gcgcggatga gtcagaaacg ctcagacaac tggaagcgct tcagaaaaac
     6121 agagaggaaa aacctgtaag atataaattc atttttaatg atgccacgac agcagcaatt
     6181 aaagatcagc tttgtggtca gtggcgttca gtgggtatca tgtctgatga agccggtata
     6241 atatttgatg ggtataccct gtctgagctg ccttttatta acaaaatgtg ggatggctcc
     6301 gtattgtcgg tggacagaaa gaatgaaccc gaacagatga ttgaaaatgc cagaatgaca
     6361 ttatccctca tggtacaacc cggtcttttt gaccgatata tggagcgaaa aggttctgtt
     6421 gccagggaca gtggttttct tgcccgttgt ctaatcagta aacctgccac cacgcagggg
     6481 aaacgcttta ttaacggagc tgtgaccccg ggaggatcat taacggcatt tcatgaacga
     6541 ttaatggaac tggccagagg cagtattgaa aaatccagta aggatgaacg ttattgtctg
     6601 catttttctc ctgaagcgca aaaaatattt attgatcact ataatgttct ggagcaggat
     6661 ttgtctcctt ccggaccctt aagccagttt cgggggcatg tttcaaaaaa aacagaaaac
     6721 attgcgagaa ttgctgcgtt attgcagtat ttcagtcagg gtgaagggaa aatctctgcc
     6781 gatatcatga cgtctgctgt cgtaataagt tcgtggtaca cagatgaata taaaaaatta
     6841 tttgcgttac ctgatgaatc tgaactgcag ctgcaggacg cccgggagct gctcgactgg
     6901 ctcattgagg agtgccgcgg agaatgcccg ccccgtgtca ggaaaaatta tattctccag
     6961 tgtggcccgg gccggttcag gaacaggaaa aagctgaacg tcctgctgaa cattcttgca
     7021 tctcaactca ggctctctgt cgtgcaggaa ggaaaaacca cctatgtcct gctttccgaa
     7081 ataaacaata tcacgctgga taaactgaat gcgtgctaca gtcagaaact gataagatgg
     7141 catgagcaat cctttggctt ataatgttcc ctgttgctga gacggattat tcagtggtgg
     7201 tatcgcagta tgcccgtcac cccccgcttc tgcatgaggg tgacggagga aaagtaagtg
     7261 tgcggaggcc cgggagcgtt atgaatcaac aggcgtataa caggtactcg tcaatcatca
     7321 gttccagctc gcgcttttcc cgtttgcgcg ccacagccca gttttcaagc tctgcatgtg
     7381 atttatgctt taactcttca gtccagggct gcatggagtg ggcacacatc agatcatacc
     7441 agcacagcca tatcagcgtg tggtagagat cacgatcgtc gaaacgccgg agcaggctgt
     7501 tcagcctgtt tgccagccgg ttttcaccca gacgatgtag ctcactgagt aaaacgccgc
     7561 ccagattaaa atgtgtaagc agcatgattt catgggtctg aatgctggtc attaaccctt
     7621 cctgaatggc aggaatgaac ggtggtgttc ccgggttgac gacctgatag agtccctggg
     7681 gggccaccga aatgatggag cctttttccg gcagaaaaaa gtgtgccagg gtcagacggt
     7741 gataaccatg aagatgcgga gaccccattc catgagcgac gataagcctc gtcacgcttg
     7801 tcatccattt ctgatgctgg atgagcagca gctcacaccg gttttcaagt ttttgcataa
     7861 tattctctcc tttagcgcgc agcaaaaggc ctccgggggc cggatcatgc ctttgctgcg
     7921 gttgaacgga taacggtcag gaattacgga cgggactgtg caatacgcgc ctgcagccag
     7981 gtttccactt cacttttcag ccagcgggaa ctgcggccca gtttgatggg ggcaggaaag
     8041 gcaccatcct tgatgagctt gtaaaaccat ttatcggtca ggccggtcag ttgagtgata
     8101 aatgccatgt cgaccatctg gtcatccatc agtgaaactg gggtattcat gaaaatgttc
     8161 ctccggatgt tgttaatctc aaccggcgtt ggtgatgaaa acaacgcacc accttcaccg
     8221 gcataacaac cggggagaaa cgtaaaaaat aatcgggaat gtgaccgtca ggagataaac
     8281 ggggggattg cggtggctga gccgtaccgg tatgtcgggt gattgttgcg aaacgatgct
     8341 tccgcacgcg cacgggagga ctttcgtgag acaaggggaa aactggcgtt ctgataattg
     8401 cagagttgtt tttgaaggcg gttgtggtgc atcagcctgt gacctgtggg caggcgacag
     8461 gtatcactgg tgggataaga ctgtctgatt ggtattataa atatataaga gagctcaaac
     8521 acatgagttc atatatgtga aataaatctg tgagtgtgat cagcagacca gtctacccag
     8581 tagcaacaga ttgcgttgta ctcacagggt tatccctgat aactggcgta taacctgcgt
     8641 acataaaacg tacctgcaat gcacctgaac agagtgcatt aagcatccgc ctgagcggta
     8701 aacgtatccg atctggtgag tgacagtctg agagcattct cagtaatcgc ccccttgtat
     8761 cctggaaatt ggttaagagc gattagattt ggcaaggtgt atgcagccag cagcttaaca
     8821 cctcagctaa ctggctggtt accttcaggt tgtcgcaatg gcatcggata tccggtgtcg
     8881 gataactaaa cgtagcgtta aggcgatatt gctgagagac cggttaagtg gtggcacggg
     8941 agtaatgagt ttggggaggg taccagaatg ccagtcaggt gcggtatgct ggcagtatca
     9001 cgttgattgc agcataaagt aacgagagac gaatggaagt taaaaccaga gcagttccgt
     9061 cgttgttcat cccgggagac gctggaaaaa gtgagttccc acacacgtta taaacttacc
     9121 cctgcggagc tggaagcctt taactctgcg gtcgatcacc ggctggcaga actgatcatg
     9181 aacaaacttt acgatcgcgt tccggcttct gtctggaaat atgtcatctg aacgttcggt
     9241 aatcacagcg gtattgcgcc aggtgttatc tgctctgtga ttcttttgct cttctctggt
     9301 tgctgcaaag ctgaatatgt tcccgttgtt ggaagccgag gtaataccgg gaacgttaag
     9361 cggtatagcg acaacaaaga attcgaaatc aaaagggaat ggtgtacgga ggtgcgggcg
     9421 gtaatgggcc gattttcggg cattaccgac ctctgctgag gttttctcgt atttaccggg
     9481 gggccggtgc gttactgtct ggcacaacaa acgcagcggg tcgctctgct ccgccgattc
     9541 ctgataatcc ctccccagta acctacggca acagtacaaa tacactgacg cctgaaacgg
     9601 taaccagaat cagcaccagc agtaacgtca gtagacagaa cagacgatcg gtcttccggt
     9661 aataaccgga aggatcgaca gctccacaat ccgtaaccag tttttacatt aaaaaaacaa
     9721 cgccggtaat tcaatcgtca gtccggagac gaccgttcgg gttatcacag agtgcatgag
     9781 acattgcctg ctggagataa cttcaggggc tcttcgcatt ttttacagcg tggttattcc
     9841 gtatgaacca tacggagatt taaccatgac ctacaaatac aaccccttct ggcaacaacg
     9901 tatccgtgag acggtgctgc gcgcactgga tgttcatccc cgcctgacgg cacttcgggt
     9961 tgatctgcgt ctcccggatg taccggcagc aacggacgca gctgtgatat cccgcttcat
    10021 caatgccctg aaagcacgaa tcgacgctta ccagaagcgt aagcgccgtg aaggtaaacg
    10081 cgtacatccc acgatcctgc attacgcctg ggcccgggaa ttcggggagc ttaaaggcaa
    10141 aaaacactat cacctcctgc tgctggtcaa ccgggatacc tggtgtcgtg ccggcgatta
    10201 ccgcgctccg ggatcactgg ccgggatgat taaacaggcc tggtgcagtg ccctgggggt
    10261 ggatgccgga tgctatgcca cgctggcgaa ttttccggcc tggccggtgg tgtggctgga
    10321 acgcggtgat gacaccggtt ttcagcaggt gctggagcgt gctgactatc tggcgaagga
    10381 gagtaccaaa gcttacggca ccggtgagcg caactttggc tgcagtcgtg gctgagcccg
    10441 acagacgact tatctgacct tagtgtgatg atgcgttcac agcgaccaca ctgattttcc
    10501 cttctgagac tgaactgaaa accgctattc acacctgacc acgctgcccg taccgggacg
    10561 gctgttgcct gcgtgtcgtc tggcggctga ggaatatgac tatgtacgca aaatcctttc
    10621 tcgctcttga tggcaacggg cgtctgaccg gtgcccgtac tgcacagact gcgccatacg
    10681 atcgttatac ctgccatctt tgcggcagtg ctctcagata ccatccgcaa tacgacactg
    10741 agcgtccctg gtttgaacac accgacgacg ggctgactga acacgctcag cagtgtcctt
    10801 acgtcaggcc tgatcgcagg gaagtgcaac tggttaaacg tttacggcaa ttcgtaccgg
    10861 atgccttacc cgtagtacgt aaagccagct ggtactgcag acaatgtcac cacgattatt
    10921 atggggagcg gtactgcaca cactgccaga ccgggcgctt cagtgaagag gggggagcag
    10981 aatgaacggt ataaaaacgg ggctggggat cacgccgggt gagcacatta tcagtgccga
    11041 ctctgcgctc agcagaaata tccggcattg tttctgcctt tcctgtcatg gccggttgat
    11101 tctgcagaca gatgctcagg gtgcgtggtt tgagcatgat ttacatgccc tgagtgagca
    11161 acagaaggcg gactgtgtgg tgctgaatcc ggaaaagtcc cacccttaca ttgaggatat
    11221 ggcgatgttt ttatcgccat taccggtcgt gctggagtgg cattgtgtga tgtgcgggag
    11281 gctttttccc gggaagaagt tctgtgaggc ctgtggtacg gggatttatt gcagagcggt
    11341 atgtacgaag tctgtttaca gctaccagcc agacttgttt gaggactgcg gaagtgcgag
    11401 tacttaatcc gaaatccccg ctctgcgcag ggcgttcaga ggtattgcca acctgtattt
    11461 tatccggtgt ttcgcaccca ccccgttcgt gacacaaagg cgcgacaccg gatatccgca
    11521 gtcctgctta cctgaataaa acccggggta ttgattggta gtgtggtgcg gaatcgtacc
    11581 gtgatctcgc attcgccgaa ttcgcactgt ggtgtcaatc aaccacacgg gggcgtcgtc
    11641 tcagcctttg cctgcctcag gagggtgaaa cctggggggc attgattaat atcctgcccg
    11701 atctgatgac cggtacttca gacctgctac cgatacgccg gaatataacg gtcagagagg
    11761 catatttcga tgcccctgtt ctggagcagt tgatgatgga tgccataggc aatgtaagcg
    11821 ctttgtatca ccggacgaag cacgcagtac cagaagcccc ccggggttca ttggtgacct
    11881 gacagcgaag atagattcaa agagcgttca gtgatttttc ctgcgctcgt cgtggtgtac
    11941 gcaatcagga atcccgttat ctcattggca tacttgcgat accacaccag caggacgacg
    12001 ctgaaatcac gccgacattc tcctgtatgg catcaatctc acaggggaca ttctgagcat
    12061 gttgacctca atcacagatc acgacagcgt gctcatttta ctgtcttata tcattggtgc
    12121 tgacgcaccg gcccgacaaa gggttttaca cgctgattca gtgaagacca tttctctaaa
    12181 cccatcaaac tggggtgaag caaccgcaag tacaaaagtg aagtgccaca gtagatacag
    12241 caacgaatca tggaggtcag aagtatgaaa cgtgttgtga taccggtacg ctggcaatgt
    12301 gcaaaatgcc agcgctggta ttgtggaacc cggccctgcc cttggtgctg gcgatattcc
    12361 cgttcatctt cctgctgata ccttccgttc agcagcgggt tgttatccct tcctgactga
    12421 ttcgtcattc cattcttatt gattagaact ggcattacac cggtgccggc gtgctttcct
    12481 gcgtgtctgc gccggttcag taaattagaa aagaaacatt tgtcagcgcc agtgatataa
    12541 ggcgtaacgg ctaacaatct tggcgttaat acattccgag caaataaccg aagcgttact
    12601 tttagtgacg gggttatcca atacttttct agtcagtgtt tagtatttat gcaagcgata
    12661 taagtagtca ccgcttagac tattgacatt catgatgcct gttaattcaa atttcaggta
    12721 tgatcagtca tttattgttg tgatactgat tccgctagtt tagcataact gtcctgcaaa
    12781 tcattgttca aaaaagaaag ctcctacaaa ctgtatcctg tatctctacc agaagataat
    12841 atacttggtt atttttgtgc ataagctcac ataaaaataa tattaattat gttttcttag
    12901 agatagaaaa ataaacaggt aagcagaaga cggacttttt taatgcgaaa acgtattctt
    12961 tttattggcc caccgctgta cggtttgtta tacccattga tttctctggc tcaggccttt
    13021 cgtgtaattg gacatgatgt agtaattagt agcgctggca aattcgcgaa taaagcagca
    13081 gaagctggac tggttgtttt tgatgcagtt ccaggtttag attcagaggc tggatatcgc
    13141 catcaggaag agttgaggaa aaaaagtaat attattggtc atttctcttt ttttagcgat
    13201 gaaatggcag ataacctcat cgattttgca ggaaaatgga ggccagattt aatagtctat
    13261 ccccctcttg gtccggcagg cccattggtt gctgctaaat atagaattcc ttcagtgatg
    13321 ctggctgttg gattcgcgca tacatctgcc catattcaga tgttaaaccg ttctttaagc
    13381 aatgcttaca ggcggcatgg agtcagcggt ccactatgtg atttagcatg gattgatgtt
    13441 gctcccccaa gtatgagcat tcttaaaaat gctggggaac cggttatctc aatgagatat
    13501 attccttata acggaggtgc tgtaaaggaa acatggtggg acagggattc tgatcggaaa
    13561 cgtttactta tcagccttgg cactgtaaaa ccaatggttg atggtctgga gctgatttca
    13621 tgggttatgg attctgcaaa tgaagttgat gctgatatca ttttgcaact tgcaataaat
    13681 gctcgtactg gtttacgaaa actaccatca aatgtacgtc tggttgactg gatacctatg
    13741 ggtgtattcc ttaatggagc tgatggattt attcatcacg gtggcgcagg taataccctg
    13801 acagcgctgt atagtggaat accacagatt gtgtttggcg aaggtgcaga tcgctctgtt
    13861 aatgcagaaa ttgttgcgaa gcgtgggtgt gggattattc cggacaagca tggactgacc
    13921 agtgatttgg taaatcgcct gctttatgat gattcactac gcttctgttc agatcaggta
    13981 gccgctgaaa tggctgaaca acccagtcct gcagagattg cagaggtttt gatgagaaaa
    14041 ttaaaaaaca acgggaaaca attgtagcgt attgctgaaa ttttcaacga gaaatgttat
    14101 gagtaatata atgtccttca tcacataaat gaaggacatc actaaataaa tgagatagtc
    14161 tcccataata atattgcaga aagagttatc ggacgataaa acgatatttt acacagagtt
    14221 attatttgat accttcctgc aattataatt tgctaattca tcaataattg ctccgcgcca
    14281 ccaagcataa tcgtgaccac cggtatagac agtgaggtta ctttccaaac cagctgcgat
    14341 taacgaatga tgcaatcgct gaacatgtga gacggttgta ccttctaaag aaccgactcc
    14401 aagtcggata tttacatctt tcggaggcgc tgaaagtatc tgctcactta cccaggaggt
    14461 attattctca gtaaacaaga tcgggctgcc ctggtcaggg ttccaccaca ttgaaggtga
    14521 gtgactaatg attgtaccaa atgttgtcga cgcatatata gctgccatca gtgctgtcac
    14581 tccaccgagg ctctgcccgg ccagtacagt attagagcga ccagcccata cgatatccgg
    14641 atagtctctg tataactggg gtatcagatt ttccgctata tcaaagatga gttctttatt
    14701 gcctcccagt atattcatac gatcagattc attaatattg tctatcccca taattgccat
    14761 tggcgtgata cgaccagtgg tgatggccat atctaatgcc cgggtaatat ctatccggtc
    14821 aaaccatgtt tcagcatcag gtaacacaag taatccaaga ggtactgatg ttggattcag
    14881 gggaaaatat aaacgaatcc ggcgttgata tcctgcaaca aaggaatgta atgttgaaag
    14941 aatacctgta catttatggg aagtatcatc ccattccttt tgttcaggag ccatatcaag
    15001 agaaaggact gattctccga atcctcgtat atttatttct gctgttttgt taaatggatc
    15061 agatttaccg ggtaatggag agaagcgact tcctagttga aatatgtctt tttgtgtcat
    15121 atctgtggga atttctataa atgagtatga gccccgatat gaagctggta acaccagtgt
    15181 cagcatccag atatctgtcg aagggatatg agtcattagt ccttttttga cgtctttttt
    15241 atctgtaaca cgattcaggc gaagatatac gccttgcagt gctttctctg agcgccacag
    15301 aaatgtaacc tcccgatttt ctgtagtggt gctttttgtg atcactggaa gtgttgctga
    15361 ctcaagatag tgccagaaat tagcaatatc tgttgttctt attgtgacag gatgtgtcat
    15421 tttttttaac tgtacacaaa tatcttcggg tgtctggaaa agatagtttt tcataggtat
    15481 catcttaaat taatccaagt gtaagaataa tacatcaaat gacgttcatt caagggatga
    15541 tgagtgactt tccggaatta aatc
//
LOCUS       DAZFQD010000126        15423 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.126, whole genome shotgun sequence.
ACCESSION   DAZFQD010000126 DAZFQD010000000
VERSION     DAZFQD010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15423
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.126"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(170..1081)
                     /gene="gsiD"
                     /locus_tag="ACHKTW_003956"
     CDS             complement(170..1081)
                     /gene="gsiD"
                     /locus_tag="ACHKTW_003956"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HFO6464883.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGAAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(1084..2004)
                     /gene="gsiC"
                     /locus_tag="ACHKTW_003957"
     CDS             complement(1084..2004)
                     /gene="gsiC"
                     /locus_tag="ACHKTW_003957"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HFO6464884.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMLLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(2022..3560)
                     /gene="gsiB"
                     /locus_tag="ACHKTW_003958"
     CDS             complement(2022..3560)
                     /gene="gsiB"
                     /locus_tag="ACHKTW_003958"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0034634 - glutathione transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HFO6464885.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGLTYTVKLREGIKFQDGT
                     DFNAAAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPAALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQATLLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            complement(3580..5451)
                     /gene="gsiA"
                     /locus_tag="ACHKTW_003959"
     CDS             complement(3580..5451)
                     /gene="gsiA"
                     /locus_tag="ACHKTW_003959"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HFO6464886.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLQRRSREVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYPHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQAPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKEAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGPRRAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(5438..6403)
                     /gene="iaaA"
                     /locus_tag="ACHKTW_003960"
     CDS             complement(5438..6403)
                     /gene="iaaA"
                     /locus_tag="ACHKTW_003960"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFO6464887.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFARGMERVSPEIFSTPLRYEQLLAARKEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            6607..7842
                     /gene="moeA"
                     /locus_tag="ACHKTW_003961"
     CDS             6607..7842
                     /gene="moeA"
                     /locus_tag="ACHKTW_003961"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HFO6464888.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTAQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASRQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGNGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKTILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLATLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VDVGEWVEVEPFNALFGGL"
     gene            7842..8591
                     /gene="moeB"
                     /locus_tag="ACHKTW_003962"
     CDS             7842..8591
                     /gene="moeB"
                     /locus_tag="ACHKTW_003962"
                     /EC_number="2.7.7.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HFO6464889.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLVVGLGGLGC
                     AASQYLASAGVGNLTLLDFDTVSLSNLQRQTLHSDATVGQPKVESARYALTRINPHIA
                     ITPVNALLDDAELAALIAEHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(8770..9432)
                     /gene="fsa"
                     /locus_tag="ACHKTW_003963"
     CDS             complement(8770..9432)
                     /gene="fsa"
                     /locus_tag="ACHKTW_003963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFO6464890.1"
                     /translation="MELYLDTSYVVAVKALSRIFPLAGVTTNPSIIAAGKKPLDVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDARKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVEAAVAKFEQDWLGAFGRT
                     SI"
     gene            9563..10462
                     /gene="ybiY"
                     /locus_tag="ACHKTW_003964"
     CDS             9563..10462
                     /gene="ybiY"
                     /locus_tag="ACHKTW_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HFO6464891.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMTTVLRDKPFYDRSGGGLTLSGGEPFMQPEMAMALLQASHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            10468..12900
                     /gene="ybiW"
                     /locus_tag="ACHKTW_003965"
     CDS             10468..12900
                     /gene="ybiW"
                     /locus_tag="ACHKTW_003965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HFO6464892.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSE
                     HIERFAALAREMAATETRESRRDELLTMAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIEMLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQNLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVQVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            13046..13861
                     /gene="ybiV"
                     /locus_tag="ACHKTW_003966"
     CDS             13046..13861
                     /gene="ybiV"
                     /locus_tag="ACHKTW_003966"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HFO6464893.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPHFMAQYQELKKRGIKFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKAVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            14013..15278
                     /gene="ybiU"
                     /locus_tag="ACHKTW_003967"
     CDS             14013..15278
                     /gene="ybiU"
                     /locus_tag="ACHKTW_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HFO6464894.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLDRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRRPPGTTSKGLGAHTDSGALERWLLPAYQ
                     RVFANVFNGNLAQYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSDMLPGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGMDV"
BASE COUNT         3722 a         4266 c         3939 g         3496 t
ORIGIN      
        1 atttaacaaa ataactatct gaacgctgtg ccgctgcaaa aatagtccct tgctgcggct
       61 ttttaaccaa attacccgat tctacaaaat cagcagaaag ttacaacact tgcttagggc
      121 aataattgcc tgtgaggtga ttaccctttc aagcaatatt caaacgtaac tatcctttaa
      181 ttttcggatc cagcgcatcg cgtaaaccat cgcccaacaa attgaacgcc agtacggtca
      241 gaaaaatagc cagggccgga aaaacagcga catgcggcgc gataaccata tccgctcgtg
      301 cctcattgag cattgctccc cactctggtg tcggcggctg cgcaccgagg ccgagaaatg
      361 acaggctggc ggcagagata atcgaggtgc caatgcgcat ggtgaaaaac accacgatag
      421 acgagacggt cccaggcaag atatgacgca ataaaatggt catatcgctg gcaccaatac
      481 tgcgtgctga ctcaataaag gtttgctgtt tcaacaccag cgtgttgccg cgcaccaggc
      541 gggcaaacgc ggggatggaa aaaatggcga ctgcgataat cacgttagca atgccgcttc
      601 ccaacaccgc aacaacagcg atcgccagta aaatacccgg gaaggcaaac agcacatcgc
      661 aaatgcgcat gatcagccga tcccaccagc cttcataata tccagccagc aagcccagca
      721 acgtcccgat cgccgcaccg ataaacacgg caaacacgcc cgccgccagc gagatttgcg
      781 caccaaccag gacacggctg aaaatgtcgc gccccagtga atcgacgcca aaccagtgct
      841 gcaaagaagg tccgttattc agattgtcat aatcaaaata attttcggcg tcatagggag
      901 cgatccagcg tgcaaaaatg gccaccacaa tcaataaaat aacgaataat gcggcggtca
      961 tcgccatatg ctgacggcga aatcgtcgcc agaattcatg ccacggtgta cgcacctggt
     1021 caggtttgac cagtggcatg gcgtttaaca ccgcctgacg tcgccagtta aatagtcgca
     1081 tccttacttg tacctgatag ccgggttaat ggcggcgtaa agcacatcca ccactaagtt
     1141 gataagaata aattccagcg agaaaagcag aatttccgcc tgaatcaccg ggtaatcgcg
     1201 catttctacg gagtcaacga gtaagcgtcc aagtccgggc cagttgaaaa ctttctccac
     1261 cacgatagaa ccaccgagca aaaagccaaa ctgtaagccc atcatggtca ctaccgggat
     1321 catcgcgtta cgtagcccgt gtttgaggac aacccaggtt tcgctcaccc ctttcgccct
     1381 cgcggtacgc atataatctt cgcttaaaac atcgacaaac gacgcgcggg taaagcgcgc
     1441 catcacggct gccactgccg cgccgagcgt cagggagggt aaaatgtagt gctgccaact
     1501 gtctgctccc acggtaggca gccagcccag ttcaacggag aatacctgaa ttaaaagcat
     1561 ccccagcgca aatgccggaa acgagatccc cgacaccgca atggtcatac tcaatcgatc
     1621 cggccaacga ttacgccaga cggcggcgat aattcccgcc gccataccaa atatcaccgc
     1681 ccagaccata ctggttatgg tcagccacag cgttggcata aagcggctgg caatctcatc
     1741 ggcaaccgga cgacgcgaca ccatcgacag gccaaaatcc ccctgcacag cattgctgat
     1801 atagtgccag aactggtgat acagcggctg atccaacccc agctgctgac gcaccagttc
     1861 tataacctgc gcatcagctt cgggcccggc aatcaatcgc gccggatcgc cgggcagcat
     1921 atgcacaaat aaaaacacca gcaccgagac gataaacagc gtcggaatca accccagtaa
     1981 tcgtttgata acgtaattaa gcattccact ccctgcgttg cttattgcaa atccgcgtct
     2041 tcaaagctga agccggtgtc tggcatgatc caaaaaccgg tcaggttttt actgtgtgcc
     2101 gacaccagtt tttctaccac cagcgggatc cacggcgact cttgccagat gatatcctgc
     2161 gccgccttat acaagcgggt cttttccgcc ggatcattag ttttcagtgc ctgagccagg
     2221 aagtcgtcca cctgtttatt gctgtaaaat gcggtattaa acagcgtcgg tggccagttt
     2281 tgcgaggcga acagtggcga tagcgcccag tccgcttcgc cggttgaagc cgaccagcca
     2341 gtgtagaaca tccgcacgcc gctctctttt tgccctttac cttcaacttc tgccgcccgc
     2401 tgtccggcat ccatcgcggt cacctgggct ttaatcccga cctgcgctaa ctgctgctgg
     2461 gtaaattgca gcactttctg cgcggtgctg tggttatgtg acgaccacag cgtggtactg
     2521 aaaccgttgg gatatcccgc ctctttcagt aattcgcgcg ctttcactgg atcgtaaggc
     2581 cacggtttat aactttgcgc gtaggcgata cttggcggta ccacaccagt agctggcgtt
     2641 gcatagcccg caaaggcaac tttcaccagc gccggacggt taatggcgta attcagcgcc
     2701 tcacggacct tcgggttatc gaacggcttt tgcgtcacgt tcatactgat ataacgctgc
     2761 ataattgacg gactggccat caactcgata tttttgtttt tctccagcag tgtggcctgc
     2821 tcgtagggaa tggggaaagc aaactgcgct tcaccggttt gcagcattgc cgcgcgggtg
     2881 ttgttatccg ccaccggacg ccaggttatg ctgtccagtt tgggcaatcc tggctgccag
     2941 taacccgcga attttttcac cttcacaaaa tcggtctgat tccaggtatc cagttcatac
     3001 ggtccggttc ccaccggatg aaaaccaatc tccttgccat atttttccag cgctgccggt
     3061 gaaatcatcg cggtcgccgg atgggcaaga atattaataa acgctgagaa cggctgtttg
     3121 agagtaatct ttactgttgt tggatcgatc gcctccgttt tggcaatatt cttatacagg
     3181 ttatagcgtt taagatgatt cgccggatcg ctggcccggt ccagattcgc ttttaccgcc
     3241 gctgcgttga aatcggtgcc atcctggaat ttaattcctt cccgcaattt cacggtgtat
     3301 gtaaggccgt catcggaaac ggtataactc tccgccagca cgtttttcag tttcatctct
     3361 ttatccagac caaacagccc ctggtaaaac gatttcgcta cggcctgaga taacgtgtca
     3421 tttgcgtcat acggatcgag cgtggtgaag ttcgatccta ccgccaccac cacatctttg
     3481 gcagcgaatg cacaagatgc catcaacgct gtcgcaatgc ccaacgccac taacccacta
     3541 cggtgtacag ctcttgccat tttattctcc gcctgaatgt tatctacgca tgaatgcgta
     3601 ttctgattgt ggttgtgcga cgtaatgccc cggcccgacg cattgcaacg agacggctgc
     3661 cacctcttcg ccacgcagat gaatattgct gggaagatcg tccgacagca gtacacgctg
     3721 cggtcgttgt cgggacggtt cagcgaccgg aactgccgcc agtaatttac gcgtataagg
     3781 atgctgcggg ttttcgaaga ccgcgcgccg tggaccaatt tcaacaattt gtccgagata
     3841 catcaccgcc acacgatgac taatccgctc taccacggcc atatcgtggg agataaacag
     3901 atacgcaatg ccgaaatcac gctggagatc gagcaacaag ttgataatct gcccgcgaat
     3961 agaaacatcc agcgccgaga cggcttcgtc ggcaatgatc actttcggat tcaatgccaa
     4021 cgcgcgagca atgcagatgc gctggcgctg accgccggaa aactcatgcg ggtaacgcca
     4081 ggcatgttca ggtaacaggc ccacgcgctc cagcaaccac gcaacgcgtg cagccgcttc
     4141 tttacctggc aataaaccgt gtacacgcag cggttcgata atcgaatcac cgatggtctg
     4201 acgtgggtcc agcgaagcgt aagggtcctg aaaaataaac tgaatatccc ggcgcaatgc
     4261 ctgaagtttg ccgggtgaca aggtatcgat tctctgaccg ttaaagataa tttcgccgcc
     4321 ctgcgattcg accaggcgca gcaacgcccg cccggtagtg gatttaccgc tgccagactc
     4381 gcccaccagc gatagcgttt cgccaggcca gagatcaaaa ctgactttct caacggcatg
     4441 cacttcccgc gttacgcgat tcaacaaacc gctgcgcaaa gggaaacggg tgacaagatt
     4501 acgcactcgt aaaacaggtt cgccatccac caccgttttc tgctcgatgg ggggggcctg
     4561 tttcgctgga tgttcaagcg atatcaacgg gaaacgtcgg ggataatcta accctttcat
     4621 cgcaccaagt tgcggaacag cagctaacag cgcacgggtg taaggatgtt gcggtgcatg
     4681 aaaaatctgt tcgacggtac ccgtttccac cgcctcgccc tgatacatca ccagtacccg
     4741 atcggcaatc tctgccacca cgcccatatc gtgagtgata aagataacgc ccatcgacat
     4801 ctctttttgc aatactttga ttaattgcag gatctgcgcc tgaatagtga catccagcgc
     4861 ggtggttggc tcatcggcaa tcagcaccgc cgggcggcat gacagcgcca tcgcaatcat
     4921 cactcgctgg cgcatcccgc cagagagttg atgcggataa cgtgaaagaa tggtttgtgc
     4981 ctcaggaatg cgtacctgat ccagcatccg cttcgcctcg accatcgctt cttcacgact
     5041 ggcgttctga tgcagacgaa ttgattcggc aatctgttca cccacagtaa ataccgggtt
     5101 cagcgatgtc atcggctcct gaaatatcat cgccatatcc gcaccgcgaa catggcgcat
     5161 ttgtgcagcg ctctgctcgc taagttcaat cacttcgcga ctgcgccgct gcaacagcat
     5221 tttatcgcac tgtactaaac cgcccgcctg ttccaacagg cgcatcaatg ccaacgcagt
     5281 cactgactta ccggagccgg attcgccaac aattgccagc gtctcaccgc gttgcagact
     5341 aaaagagaga ttgcggaccg cagctatttt ctgctggtcc tgcataaagg caatattcag
     5401 attttcaacc gccagcacat taccggcatc aagttcatca ctgtgtggca acggtgtccc
     5461 ctttttcacg gtagataccg gtggttggcg tatcgcctgc gtagccccag gcgcgataca
     5521 ttccttcggt gttaaacggt agcgcgacat tcccttcatg gtcgatagcg attaagccac
     5581 cgctaccgcc aagcgcaggg agtttttcca ttactacccg ctcgcaggct tccgcgagac
     5641 ttaatccgcc gtaatccatt aacgcggcga tgtcatatgc cgccagcgcg cggatgaaga
     5701 cttcgcccgt gccggtacaa gaaaccgcca cactggcgtt attggcgtag catccggcac
     5761 ccactaaggg actatcgcca actcgtccgg gtaatttatt ggtcattccg cctgtggacg
     5821 tggctgccgc caaattgccg tctaaatcca acgccacggc ccccacggtg cccattttct
     5881 gtttttcatc cagtggcgca ccgctatggt cgaggacggt tgccccttcc ttgcgcgctg
     5941 ccagtagttg ttcataacgc aaaggcgtgg agaaaatctc cggcgagacg cgctccatgc
     6001 cacgagcaaa cgcaaaattt tctgcccctt cgccaatcat catcacatgc gggctttgct
     6061 ccatcaccag ccgggcggca agaaccggat tacgcagatg actaacgccc gccaccgcac
     6121 cggctttcag ggtgttacca tccatcacac aggcgtccag ttcatgggtt tcatcacgcg
     6181 taaagacagc gccaattccg gcgttaaaca gtggacactc ttccagcaga cgcaccgctt
     6241 ccgtcaccac atccagcgca ctttcgcccg cttccagcat tttctgcccg gtttcaacaa
     6301 tggcagacaa cgcctcaatg tagcgtaatt cctgttgcag actcatctgc gcgcggctaa
     6361 ttgcacctgc gccaccatga attgcaatga ctgctttgcc catagtattc gtccattaag
     6421 ggtggctcgc cacttttgct ataaatatca gaatttttat gaattactca ttcaattttt
     6481 gaatatagaa agatggatat ggcatgtaaa ggcaggagcc aacgctacgc ccgaggtggc
     6541 atacagtctg tggcgttttt ctgacataat aggcaaattc gattttgcct ccgaaggagt
     6601 gttttcatgg aatttaccac cggattgatg tcgctcgaca ccgcgcttaa tgagatgctt
     6661 tctcgcgtca ccccactgac cgcccaggaa acgctgccac tggtacagtg ttttggtcgt
     6721 attctggcga gcgatgtcgt ttcgccgctt gatgttccgg ggtttgataa ctccgcaatg
     6781 gacggctacg cggtgcgttt agccgatatt gcctccaggc aaccgctgcc cgttgccggt
     6841 aaatcctttg ccggtcagcc gtaccatggt gaatggcctg cgggtacctg cattcgtatt
     6901 atgaccggtg cgccggtgcc ggaaggctgc gaagcggtgg tgatgcagga gcagactgaa
     6961 caaacggaca atggcgtgcg ttttactgct gaagtgcgta gcgggcaaaa tattcgccgt
     7021 cgcggtgaag atatctctgc aggtgcggtt gttttcccgg cgggaactcg cctgactacc
     7081 gctgaactgc cagtgattgc ttcactgggg attgccgaag ttccggtgat tcgtaaagtg
     7141 cgtgtagcgc ttttttctac cggtgatgaa ctccagttgc ccggtcagcc actgggcaat
     7201 ggccaaatct acgataccaa ccgtctcgcc gtacacctga tgttagaaca gttgggatgc
     7261 gaggtaatca acttagggat tatccgcgac gatccacatg ccctgcgcgc cgcatttatt
     7321 gaagccgaca gccaggcgga tgtggtgatc agttccggcg gtgtttcagt gggtgaagcg
     7381 gattacacca aaaccattct tgaagagctg ggggagatcg ccttctggaa gctggcgatt
     7441 aaaccgggta aaccgttcgc gttcggtaaa ctttctaata gctggttctg cggcctgccg
     7501 ggcaacccgg tttcagcgac gctgaccttc tatcaactgg tacagccttt gctggcaaca
     7561 ctaagcggca acaccgccag cggcctgccc gcgcgccagc gcgtacgcac agcgtcccgc
     7621 ctgaaaaaaa cgccaggacg tcttgatttc cagcgcggcg tgctgcaacg caacgccgac
     7681 ggtgaacttg aagtgacgac caccggacat cagggttcac atatatttag ctcctttagt
     7741 ctcggcaact gctttatcgt gctggaacgc gatcgcggca atgtggacgt gggcgaatgg
     7801 gtggaagtag aaccgtttaa cgcgttgttc ggaggcctgt aatggcggaa ctcagcgatc
     7861 aggagatgct gcgctacaac cggcaaatca ttctgcgcgg ttttgatttt gacggtcagg
     7921 aggcgctaaa agattctcgt gtgctggtag tcggcctcgg tggactcggc tgcgcagctt
     7981 cgcagtatct ggcaagcgcc ggtgtcggca acctgacgct gctcgacttc gacacggttt
     8041 cgctctcgaa tctgcaacgc cagacactgc acagtgatgc cacggtcggg caaccgaagg
     8101 tggaatccgc ccgttacgcc ctgacgcgga tcaacccaca tatcgcgatt acgccagtca
     8161 atgcactgct agatgacgca gaacttgcag cattgattgc tgaacacgat ctggtgctcg
     8221 actgtacgga taacgttgcg gtacgtaatc aactgaacgc aggctgtttt gccgcgaagg
     8281 tgccgctggt ttccggcgcg gcaattcgta tggaagggca aatcacggtc ttcacttatc
     8341 aggacggcga accgtgctat cgctgcctta gccgtttgtt tggcgagaac gcattaacct
     8401 gcgtagaagc aggcgtgatg gcaccgttga tcggcgtaat tggttcgttg caggcaatgg
     8461 aagcgatcaa actgctggca ggttatggca aaccggccag cgggaaaatc gtcatgtacg
     8521 atgcgatgac ctgtcagttt cgcgaaatga aactgatgcg caatccgggg tgtgaggtgt
     8581 gcgggcagta attgccacct ccaggcctga taagacgcga caagcgtcgc atcaggcatt
     8641 tgctccagac gccagatgcg gcgtgaacgc cttatctggc ctacggttcg ggcacagatt
     8701 tccaagcgcg gcaggcatcg gtgcactccg ccacgtcatc agcaatttat gaggatgcag
     8761 aacgtccggt taaatcgacg ttctgccaaa cgctcccagc cagtcctgtt caaacttcgc
     8821 cacagcggct tccaccgccg gatagctaat catctgttgt gccacatcca gtggcagcgt
     8881 aattgattca catcctgcca gtaagcagtc cagcgcctga cgcggggttt tgaaactcgc
     8941 tgccagcact ttcgcctgcg gcgcatgcat tttcaataac tggtgtaagt cggtcacagt
     9001 ctgaatgccg ctaccgccct gagcatcaat acgattaacg taaggcgcaa catattccgc
     9061 acctgccagc gccgacagca gcccttgtgc tgcgccatat accgcggttc ccagcgttgg
     9121 aatcccttcc gcttttaaca tcttaatagc tgccagcccc tcggcggtca ccggaacttt
     9181 caccacgata tccgcaataa tagaacgcaa tttacgtgcg tcattcacca tcccttcagc
     9241 agtggtagcc attacctggg caaacagacg cccctgaccg cccatcgctt catgaagttg
     9301 cggaagcaca acatccagcg gttttttacc cgcggcgata atgcttgggt tagtggtcac
     9361 acccgccagc ggaaaaatac gtgacagcgc cttcaccgca acaacgtatg aagtatccag
     9421 atacagttcc ataaccatcc tcaaaatgta ctttttgtct gaatgtagac tatcacggga
     9481 aaactctctg aacagttgac gcacatcaat ctaactttca ttcgaaagta ttttaatgtt
     9541 tgaacgaaac agagaggcaa ctatgatttt caatattcag cgctactcga cccatgatgg
     9601 ccccggtatc cgcacggtcg tatttcttaa aggctgttcg ctgggctgcc gctggtgtca
     9661 gaacccggaa agccgcgccc gcacgcagga tctgctgtat gacgcacgac tgtgtctgga
     9721 aggctgcgag ctgtgcgcta aggctgcgcc ggaagtgatt gagcgcgcgc tgaatggttt
     9781 gcttattcat cgggaaaagt taaccccgga gcatctgacg gcgttaaccg actgctgtcc
     9841 gacacaggca ttaaccgtgt gtggtgaagt gaaaagcgtt gaggagatca tgacgaccgt
     9901 tctgcgcgat aaaccgtttt acgatcgcag cggcggcggt ttaacgcttt cgggtggtga
     9961 gccctttatg cagccggaaa tggcgatggc gctactgcaa gccagccacg aggcaggcat
    10021 tcatactgcg gtagaaacct gtctgcatgt gccgtggaaa tatatcgccc cttctctgcc
    10081 ctatatcgat ctgtttcttg ccgatttaaa acacgttgcc gacgcgccgt ttaaacagtg
    10141 gaccgacggt aacgccgcca gagtgctgga taacctgaaa aaactcgccg cagcgggcaa
    10201 aaaaatcatt atccgcgtgc cgctgattca gggctttaat gccgacgaaa cctctgtaaa
    10261 agccattacc gattttgccg ccgacgagct gcacgttggc gaaattcatt ttctgcccta
    10321 ccacacgctg ggcatcaaca aatatcactt acttaatctg ccctatgacg ccccggaaaa
    10381 accgcttgat gcgccagaac tgctcgactt tgcccagcag tatgcctgcc agaaagggtt
    10441 aaccgcgacc ttacgaggat aacaaccatg accacactga aactggacac gctcagcgac
    10501 cgcattaaag cgcacaaaaa tgcgctggtg catattgtga aaccgccagt ctgtaccgag
    10561 cgcgcgcagc actataccga gatgtatcaa caacatctcg ataagccgat cccggtacgt
    10621 cgcgcgctgg cactggcgca tcacctggcg aatcgcacca tctggatcaa acacgatgag
    10681 ttgatcattg gcaaccaggc aagcgaagtt cgcgccgcgc cgatcttccc ggaatatact
    10741 gtctcgtgga tcgaaaaaga gattgatgat ctggcagatc gtcccggtgc tggctttgcg
    10801 gtgagcgaag agaacaaacg cgttctgcat gaagtgtgcc cgtggtggcg cggtcagacc
    10861 gtacaggatc gctgctacgg catgtttacc gatgagcaaa aaggtctgct ggcgaccgga
    10921 atcattaaag cggaaggcaa tatgacctcc ggcgatgcgc acctggcggt gaatttcccg
    10981 ctgctgctgg aaaaaggtct tgatggtctg cgtgagaaag tagcggaacg tcgctcgcgc
    11041 atcaatctga cggtgctgga agatttacat ggcgaacaat tcctgaaagc gattgatatc
    11101 gtgctggtgg cagtcagtga acacattgaa cgtttcgctg ccctggcgcg tgaaatggct
    11161 gctaccgaaa ctcgcgaaag ccgtcgcgat gaactgctga cgatggcaga aaactgcgat
    11221 cttatcgccc accagccgcc gcagactttc tggcaggcgc tgcaactgtg ttacttcatc
    11281 caattgattt tgcagatcga atctaacggt cactcggtat cgtttggtcg tatggaccag
    11341 tatctctacc cgtactatcg ccgcgacgtt gaactcaacc agacgctgga tcgcgaacac
    11401 gccatcgaga tgctgcatag ctgctggctg aaactgctgg aagtgaacaa gatccgctcc
    11461 ggctcacact caaaagcctc tgcgggaagt ccgctgtatc agaacgttac cattggcgga
    11521 caaaatctgg ttgatggtca accaatggat gcagtgaatc ccctctctta cgcgatcctc
    11581 gaatcctgcg gtcgcctgcg ttccactcag cctaacctca gcgtgcgtta ccatgcagga
    11641 atgagtaatg atttcctcga cgcctgcgta caggtgatcc gttgcggctt cgggatgccg
    11701 gcgttcaaca acgacgaaat cgtgatcccg gaatttatta aactcggtat tgaaccgcag
    11761 gacgcttacg actacgcagc gattggttgt atcgaaaccg ccgtcggtgg caaatggggc
    11821 tatcgctgta ccggcatgag ctttatcaac ttcgcccgcg tgatgctggc ggcgctggaa
    11881 ggcgggcgtg atgccaccag cggcaaagtg ttcctgccac aagaaaaagc gttgtcggca
    11941 ggcaacttta acaatttcga tgaagtaatg gacgcgtggg atacgcaaat ccgttattac
    12001 acgcgtaaat caatcgaaat cgaatatgtc gtcgacacca tgctggaaga gaacgtacac
    12061 gatattctct gctcggcgct ggtggatgac tgtattgagc gagcgaaaag tatcaagcaa
    12121 ggcggcgcga aatatgactg ggtttctggc ctgcaggttg gcattgccaa cctcggcaac
    12181 agcctggcgg cagtgaagaa actggtattt gaacagggcg cgattggtca gcaacagctg
    12241 gctgccgcac tggcggatga cttcgatggc ctgactcatg agcaactgcg tcagcggctg
    12301 attaacggtg cgccgaagta cggcaacgac gatgataccg tcgatacgct gctggctcgc
    12361 gcttatcaga cctatatcga cgaactgaaa cagtaccata acccgcgcta cggtcgtggt
    12421 ccggttggcg gcaactatta cgcgggtacc tcgtctatct ccgctaacgt accgtttggc
    12481 gcgcaaacta tggcaacacc ggacgggcgt aaagcccaca ccccgctggc agaaggcgca
    12541 agcccggctt ccggtactga tcatcttggc cctactgcgg tcattggctc ggtgggtaaa
    12601 ctgcctacgg cagcgattct cggcggcgtg ttgctcaacc agaaactgaa tccagcaaca
    12661 ctggagaacg aatctgacaa gcagaaactg atgatcctgc tgcgcacctt ctttgaagtg
    12721 cataaaggct ggcatattca gtacaacatc gtttcccgcg aaacgctgct ggaggcgaaa
    12781 aaacatccgg atcagtaccg cgatctggta gtgcgtgtcg cgggctattc cgccttcttc
    12841 accgcgctct ctccagacgc acaggacgat atcatcgccc gtactgaaca tatgctgtaa
    12901 tctcctcggc ccggcggcgt cgtcgccggg ctaaatcatt cacatcatca atttcatcct
    12961 tactttcatt cgaaatataa tttgtgctct gcgtcacatt gttattacac tgttacggtt
    13021 tcattttcga gccaggagca cacctatgag cgtaaaagtt atcgtcacag acatggacgg
    13081 tacttttctt aacgacgcca aaacgtacaa ccaaccacat tttatggcgc aatatcagga
    13141 actgaaaaag cgcggcatta agttcgttgt cgccagcggt aatcagtatt accagcttat
    13201 ttcgttcttt cctgagctaa aggatgagat ctcttttgtc gcggaaaacg gcgcactggt
    13261 ttacgaacat ggcaagcaac tgttccacgg cgaactgacc cgacatgaat cgcggattgt
    13321 tattggcgag ttgctaaaag ataagcaact caattttgtc gcctgcggtc tgcaaagtgc
    13381 atatgtcagc gaaaatgccc ccgaagcatt tgtcgcactg atggcaaaac actaccatcg
    13441 cctgaaagct gtaaaagatt atcaggagat tgacgacgta ctgttcaagt tttcgctcaa
    13501 cctgccagat gaacaaatcc cgttagtgat cgacaaactg cacatagcgc tcgatggcat
    13561 tatgaaaccc gtcaccagtg gttttggctt tatcgacctg attattcccg gtctacataa
    13621 agcaaacggt atttcgcggt tactgaaacg ctgggatctg tcaccgcaaa atgtggtagc
    13681 gattggcgac agcggtaacg atgcggagat gctgaaaatg gcgcgttatt cctttgcgat
    13741 gggcaatgct gcggaaaaca ttaaacaaat cgcccgttac gctaccgatg ataataatca
    13801 tgaaggcgct ctgaatgtga ttcaggcggt gctggataac acatcccctt ttaacagctg
    13861 acctctctac cggagccgtg cgctccggtt ttcccttctt tttacatcct gtacattaca
    13921 tcaaatcatt aaacttgcat taacttattt ataacttata ttgtcacatg caaatcagaa
    13981 tggtttcgaa tgaaagttaa tgagaggtgg ttatggcttc tacttttacc agcgacacat
    14041 tgcctgccga tcacaaagca gctatccgtc agatgaagca cgcgctgcgg gcgcagcttg
    14101 gcgacgtcca gcagatcttt aatcagctaa gcgatgacat tgccacgcga gtggctgaaa
    14161 tcaacgcact caaagcacag ggcgatgccg tctggccggt gctgtcttat gccgatatca
    14221 aagcaggtca tgttactgca gagcagcgcg aacagattaa acgtcgcggt tgtgcggtga
    14281 taaaaggcca tttcccccgc gaacaagcgc taggctggga tcagtcgatg ctggactatc
    14341 tggaccgcaa ccgctttgac gaggtctaca aaggccccgg cgataatttc ttcgggacgc
    14401 tcagcgcttc acgtcccgag atttacccca tctactggtc gcaggcgcaa atgcaggccc
    14461 gccagagtga agaaatggcg aatgcgcagt cgtttctcaa tcgtctgtgg acatttgaaa
    14521 gtgatggaaa gcaatggttt aacccggatg tgagcgtcat ctaccctgac cgtatccgcc
    14581 gccgtccgcc cggaacgacc tccaaaggtc ttggagcgca taccgactcc ggggcgctgg
    14641 aacgctggct gcttccagcg tatcagcgcg ttttcgccaa cgtctttaat ggcaatctgg
    14701 cgcaatatga tccctggcat gcggcacatc gtacggaagt tgaagagtac acggtggaca
    14761 acaccaccaa atgttccgtg tttcggacat tccagggctg gacagcgctc tctgatatgc
    14821 tgcctggtca ggggttgctg cacgttgtgc ccattcctga agccatggcg tacgtactgt
    14881 tacgtccgct gcttgatgat gtgccggagg atgaactgtg cggcgtagcg cccggaagag
    14941 tgttgccggt atcagagcaa tggcatccac tgttaattga ggcgttaacc agcattccaa
    15001 aactcgaggc cggagactcc gtctggtggc actgcgacgt catccattcc gttgcccccg
    15061 ttgaaaatca acagggttgg ggcaacgtga tgtacattcc tgcggcaccg atgtgcgaga
    15121 aaaatcttgc ctacgcgcac aaggtgaagg ccgcactgga aaaaggcgca tcgccgggcg
    15181 acttcccgcg cgaggactat gaaacaaact gggaaggacg ctttacgctt gccgacctca
    15241 acattcacgg taagcgagcg ttgggcatgg atgtttgaga gttaatggaa gtgaattgat
    15301 gtgggattaa gaaaatcgat tcgacgttac tcagattgct gacaagtgcg ctttcttaat
    15361 gcctgatgcg ctacgcttat ctggcctaca aagtcctgca gattcaatat attacaggta
    15421 ctg
//
LOCUS       DAZFQD010000127        15040 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.127, whole genome shotgun sequence.
ACCESSION   DAZFQD010000127 DAZFQD010000000
VERSION     DAZFQD010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15040
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.127"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..220
                     /locus_tag="ACHKTW_003968"
     CDS             <1..220
                     /locus_tag="ACHKTW_003968"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6464895.1"
                     /translation="INISIISSNLCLFAIIFVGLFVGYLYSKMALVHLFPPMQDDKYH
                     GRIENHQSGKSLEFHTVPFILPYHFAKF"
     gene            237..2909
                     /gene="rcsD"
                     /locus_tag="ACHKTW_003969"
     CDS             237..2909
                     /gene="rcsD"
                     /locus_tag="ACHKTW_003969"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HFO6464896.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTWQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGNNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESLPSTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVDE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRQLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
     gene            2926..3576
                     /gene="rcsB"
                     /locus_tag="ACHKTW_003970"
     CDS             2926..3576
                     /gene="rcsB"
                     /locus_tag="ACHKTW_003970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor RcsB"
                     /protein_id="HFO6464897.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(3776..6625)
                     /gene="rcsC"
                     /locus_tag="ACHKTW_003971"
     CDS             complement(3776..6625)
                     /gene="rcsC"
                     /locus_tag="ACHKTW_003971"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0018106 - peptidyl-histidine
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HFO6464898.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPGVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRES"
     gene            complement(6900..7676)
                     /gene="yfaP"
                     /locus_tag="ACHKTW_003972"
     CDS             complement(6900..7676)
                     /gene="yfaP"
                     /locus_tag="ACHKTW_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HFO6464899.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRVESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGTGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            complement(7681..9330)
                     /gene="yfaQ"
                     /locus_tag="ACHKTW_003973"
     CDS             complement(7681..9330)
                     /gene="yfaQ"
                     /locus_tag="ACHKTW_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HFO6464900.1"
                     /translation="MNWRRIVWLLALVTLPTLAEETPLQLVLRGAQHDQLYQLSSSGV
                     TKASTLPDSLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWATGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVEVELFARYPLKKITAEKST
                     TAVKPGVLNGRYRVTFTNGNHITFVSHGETTLLSEKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNANREGDCLTIPDSSATQRVSASPATTGARTMTAWT
                     QDLIYAGDPVHYHGSRATEGTLSWRQATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            complement(9331..13845)
                     /locus_tag="ACHKTW_003974"
     CDS             complement(9331..13845)
                     /locus_tag="ACHKTW_003974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0010951 - negative regulation of
                     endopeptidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin"
                     /protein_id="HFO6464901.1"
                     /translation="MGTGLANADDSLPSSNYAPPAGGTFFLLADSSFSSSEEAKVRLE
                     APGRDYRRYQMEEYGGVDVRLYRIPDPMAFLRQQKNLHRIVVQPQYLGDGLNNTLTWL
                     WDNWYGKSRRVMQRTFSSQSRQNVTQALPELHLGNAIIKPSRYVQNNQFSPLKKYPLV
                     EQFRYPLWQAKPVEPQQGVKLEGASSNFISPQPGNIYIPLGQQEPGLYLVEAMVGGYR
                     ATTVVFVSDTVALSKVSGNELLVWTAGKKQGEAKPGSEILWTDGLGVMTRGVTDDSGT
                     LQLQHISPERSYILGKDAEGGVFVSENFFYESEIYNTRLYIFTDRPLYRAGDRVDVKV
                     MGREFHDPLHSSPIVSAPAKLSVLDANGSLLQTVNVTLDARNGGQGSFRLPENAVAGG
                     YELRLAYRNQVYSSSFRVANYIKPHFEIGLALDKKEFKTGEAVSGKLQLLYPDGEPVK
                     NARVQLSLRAQQLSMVGNDLRYAGRFPVSLEGSETVSDASGHVALNLPAADKPSRYLL
                     TVSASDGAAYRVTTTKEILIERGLAHYSLSTAAQYSNSGESVVFRYAALESSKQVPVT
                     YEWLRLEDRTSHSGELPSGGKSFTVNFAKPGNYNLTLRDKDGLILAGLSHAVSGKGST
                     AHTGTVDIVADKTLYQPGETAKMLITFPEPIDEALLTLERDRVEQQSLLSHPANWLTL
                     QRLNDTQYEARVPVSNSFAPNITFSVLYTRNGQYSFQNAGIKVAVPQLDIRVKTDKTH
                     YQPGELVNVELTSSLKGKPVSAQLTVGVVDEMIYALQPEIAPNIGKFFYPLGRNNVRT
                     SSSLSFISYDQALSSEPVAPGATNRSERRVKMLERPRREEVDTAAWMPSLTTDKQGKA
                     YFTFLMPDSLTRWRITARGMNGDGLVGQGRAYLRSEKNLYMKWSMPTVYRIGDKPAAG
                     LFIFSQQDNEPVALVTKFAGAEMRQTLTLHKGANYISLTQNIQQSGLLSAELQQNGQV
                     QDSISTKLSFVDNSWPVEQQKNVMLGGGENALMLPEQASNIRLQSSETPQEIFRNNLD
                     ALVDEPWGGVINTGSRLIPLSLAWRSLADHQSAAANDIRQMIQDNRLRLMQLAGPGAR
                     FTWWGEDGNGDAFLTAWAWYADWQASQALGVTQQPEYWQHMLDSYAEQADNMPLLHRA
                     LVLAWAQEMNLPCKTLLKGLDEAIARRGTKDEDFSEEDTRDINDSLILDTPESPLADA
                     VANVLTMTLLKKAQLKSTVMPQVQQYAWDKAANSNQPLAHTVVLLNSGGDATQAAAIL
                     SGLTAEQSTIERALAMNWLAKYMATMPPVVLPAPAGAWAKHKLTGGGEDWRWVGQGVP
                     DILSFGDELSPQNVQVRWREPAKMAQQSNIPVTVERQLYRLIPGEEEMSFILQPVTSN
                     EIDSDALYLDEITLTSEQDAVLRYGQVEVPLPPGADVERTTWGISVNKPNAAKQQGQL
                     LEKARNEMGELAYMVPVKELTGTVTFRHLLRFSQKGQFVLPPARYVRSYAPAQQSVAA
                     GSEWTGMQVK"
     gene            complement(13869..14492)
                     /gene="yfaT"
                     /locus_tag="ACHKTW_003975"
                     /pseudo
     CDS             complement(13869..14492)
                     /gene="yfaT"
                     /locus_tag="ACHKTW_003975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311139.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 domain-containing protein"
     gene            complement(14489..15019)
                     /locus_tag="ACHKTW_003976"
     CDS             complement(14489..15019)
                     /locus_tag="ACHKTW_003976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 family protein"
                     /protein_id="HFO6464902.1"
                     /translation="MSKSSRGVRCVKSPEGVLPVSQTQQGEGQIWRREVSSRYGQYPK
                     AQAAQPDQLMSDYFFRVSLAMQNKTLLFSLDDTLVNNALQTLNKTRPAMVDVIPTDGI
                     VPLYINPQGVAKLLRNETLTSLPKNLEPVFYNAAQTLLMPKLDALSQQPRYVMKLAQM
                     EPGAAWQWLPITWQPL"
BASE COUNT         3649 a         4052 c         3716 g         3623 t
ORIGIN      
        1 aattaacatc agcataattt ccagcaatct ttgtttattt gcaattattt tcgttgggct
       61 ttttgtaggt tatttgtaca gcaaaatggc gcttgtacat ctatttcccc caatgcagga
      121 tgataaatat cacgggagaa tagagaatca tcaatcaggt aagagtctgg aatttcacac
      181 tgtacccttt atactgccct atcacttcgc gaagttttaa caggtcataa acacgaatgc
      241 gtcagaaaga gacaacggcc acgacccgct tttcactcct accggggagc attacccgct
      301 tctttttact gttgatcatt gtgttactgg tgacgatggg tgtaatggta caaagcgccg
      361 ttaacgcatg gctgaaagat aaaagttacc agattgtcga cattacccac gctatccaaa
      421 agcgcgtcga tacctggcgt tacgtgacct ggcagatcta cgacaacatt gccgcgacga
      481 cctccccctc ctccggcgaa ggtttacaag agacgcgcct gaaacaggat gtctactatc
      541 tggagaaacc gcgccgcaaa acggaagcgt taatctttgg ctctcacgac aactcaacgc
      601 ttgagatgac tcagcggatg tccacctatc tggacacatt gtggggcgca gaaaatgtac
      661 cgtggtcgat gtattacctg aatggtcagg ataacagtct ggtgctgatc tcaacgctgc
      721 cccttaaaga tctcacctcc ggatttaaag aatcgaccgt cagcgacatt gttgattcac
      781 gtcgtgcaga gatgttgcaa caggccaacg ccctcgatga acgcgaaagc ttttctaaca
      841 tgcgccgcct ggcctggcag aacggtcatt actttacctt gcgtactacc tttaaccagc
      901 caggacatct ggcaacggtc gtggcttttg atctgccgat taatgatttg atcccaccgg
      961 gtatgccgct ggacagtttc cgccttgagc cagacgcgac ggcaacggga aacaatgata
     1021 atgagaaaga agggacggat agcgtcagta tccactttaa cagtacgaag attgaaatct
     1081 cctcggcact caactctacc gatatgcgcc tggtctggca ggttccttat ggcaccttat
     1141 tgctggatac gttgcaaaac attctgctgc cactgctgct gaacatcggt ttgctggcgc
     1201 tggcgttatt tggctatacc acattccgcc atttctccag ccgcagtaca gaaagtctac
     1261 ccagcacggc ggtcaataac gaattgcgca ttttacgggc aattaatgaa gagatagtct
     1321 cactgctgcc gctcggcctg ctggttcacg atcaggaatc gaaccgcact gtcataagta
     1381 acaaaattgc cgatcatttg ctgccgcatt tgaatctgca aaacatcacc accatggcgg
     1441 aacagcatca ggggattatt caggcgacga tcaataacga gctgtatgag atccgcatgt
     1501 tccgcagcca ggtcgcgccg cgcacacaaa ttttcattat tcgcgatcag gatcgcgaag
     1561 tgctggtaaa caagaaactc aagcaggcgc agcgtctgta tgagaaaaac cagcaggggc
     1621 ggatgacctt tatgaaaaac attggcgatg cgctgaaaga acccgcacag tccctggcgg
     1681 agagcgcggc taaactcaac gccccggaaa gcaaacaact ggcgaatcag gcagatgtgc
     1741 tggtgcggct ggttgatgaa atacagttag cgaacatgct tgcggatgat agctggaaaa
     1801 gtgagacggt gctgttctcc gtgcaggatt taattgatga agttgtgcct tcagtgttgc
     1861 ctgccatcaa gcgtaaaggt ctgcaactgc tgattaacaa tcatctgaaa gcacacgata
     1921 tgcgccgcgg cgatcgcgat gccttacgac gtattttgct gctactgatg caatatgccg
     1981 tgacctcaac gcaattggga aaaatcaccc ttgaggttga tcaggatgag tcctccgaag
     2041 accgcctgac gttccgcatt ctggacacgg gagaaggcgt aagtattcat gaaatggata
     2101 atttgcactt cccgtttatc aaccagaccc aaaacgatcg ctatggcaag gcggacccgc
     2161 tggcattctg gctgagcgat caactggcac gtaaactggg cggtcattta aacatcaaaa
     2221 cgcgggatgg gcttggtaca cgctactctg tgcatatcaa aatgctcgca gctgacccgg
     2281 aagttgaaga ggaagaagag cgtttactgg atgatgtctg cgtaatggtg gatgttactt
     2341 cggcagaaat tcggaatatt gtcactcgcc agttagaaaa ttggggtgca acctgtatca
     2401 cacccgatga aagattaatt agtcaagatt atgatatctt tttaacggat aatccgtcta
     2461 atcttactgc ctctggcttg cttttaagcg atgatgagtc tggcgtacgg gaaattgggc
     2521 ctggtcaatt gtgcgtcaac ttcaatatga gcaacgctat gcaggaagcg gtcttacaat
     2581 taattgaagt gcaactggcg caggaagagg tgacagaatc gcctctgggc ggagatgaaa
     2641 atgcgcaact ccatgccagc ggctattatg cgctctttgt agacacagta ccggatgatg
     2701 ttaagaggct gtatactgaa gcagcaacca gtgactttgc tgcgttagcc caaacggctc
     2761 atcgtcttaa aggcgtattt gccatgctaa atctggtacc cggcaagcag ttatgtgaaa
     2821 cgctggaaca tctgattcgt gagaaggatg ttccaggaat agaaaaatac atcagcgaca
     2881 ttgacagtta tgtcaagagc ttgctgtagc aaggtagcct attacatgaa caatatgaac
     2941 gtaattattg ccgatgacca tccgatagtc ttgttcggta ttcgcaaatc acttgagcaa
     3001 attgagtggg tgaatgttgt cggcgaattt gaagactcta cagcactgat caacaacctg
     3061 ccgaaactgg atgcgcatgt gttgattacc gatctctcca tgcctggcga taagtacggc
     3121 gatggcatta ccttaatcaa gtacatcaag cgccatttcc caagcctgtc gatcattgtt
     3181 ctgaccatga acaacaaccc ggcgattctt agtgcggtat tggatctgga tatcgaaggg
     3241 atcgtgctga aacaaggtgc accgaccgat ctgccgaaag ctctcgccgc gctgcagaaa
     3301 gggaagaaat ttaccccgga aagcgtttct cgcctgttgg aaaaaatcag tgctggtggt
     3361 tacggtgaca agcgtctctc gccaaaagag agtgaagttc tgcgcctgtt tgcggaaggc
     3421 ttcctggtga ccgagatcgc taaaaagctg aaccgcagta ttaaaaccat cagtagccag
     3481 aagaaatctg cgatgatgaa gctgggtgtc gagaacgata tcgccctgct gaattatctc
     3541 tcttctgtga ccttaagtcc ggcagataaa gactaatcat ctgtaggcca gataagacgc
     3601 gttagtgtct tatctggcat ttgcaccgat tgccggatgc agcgtaaacg ccttatccgg
     3661 cctacgattc ccattatttc aacagattac attaaagtag gccagataag acgcgtcagc
     3721 gtcgcatctg gcatttgcac tgaatgccag atgcggcata aacaccttat ccgtcctacg
     3781 attcccgcga tttcctgacc ctctcggcat ataccgtcag cgtctgtttt atcacatcca
     3841 gcgttaccgg cttcgacagg cagctgtcca taccggactc cagacaccgc tgcttctctt
     3901 cagccaacgc attagcagtt actccgatta ccggcaacgt cagtcccaac tgacgaatgc
     3961 gttgcgtcaa gcggtaacca tccatatttg gcatgttgac gtcgctaagc acgatatcaa
     4021 tatgattctt gctaagtaca ttaagcgcat cgacaccatc attcgcggtt ttacattgat
     4081 agcccaacga tcccaactga tctgccagca gacgtcggtt aatcggatga tcatccacga
     4141 ccagaatcat catatcgtca ttatcgctga ccgctttgtc cgtcgacggc agagcgttag
     4201 caggatcgtc gctctccatc tcgatcaaat aaatacgcgc caacaatgcc ggtagctcat
     4261 gcggggcagc cacactgtgt acccactccc ctggcgcttt ctccagcgga ataccaatat
     4321 ggcgacgaca gaaggtcact accgctctac cctgccattt tttactcact acctcgtcag
     4381 taattaacac atcttcggga gtcggttcct gcccttcgta tgttgtaacg acgatgccgc
     4441 tgcgctgcaa actggtttcc aggaactgac agagcgacgc attgcggacc gccagccagc
     4501 agcgtttacc actcaaccct tccacgcctt ttttctgcgg gtactgagcg ccgtacaacg
     4561 gaatacgcac ggtaaactgg ctgcccattc ccggttctga atctaccgag atatcgccgt
     4621 ccatcatgct gatcagtttt tcacaaatcg ccagacccag accggtcccc tggaagtttc
     4681 gctgtacgcc cgttccgacc tggaagaagg gatcaaacaa gcgcaccact tctttcgccg
     4741 gaatccccac gccggtatcg cgaacacgga tagagagata atcgccatca gcgcggacat
     4801 gcaaaactat acagccggta tcggtgaatt ttatggcgtt actcaacagg ttggagatga
     4861 cctgctgtaa acgcatcggg tcgccattta aggccactgg cacatccggt tcaataaagc
     4921 agtacaagcc taactgcttg cgtaccacca gcggtaaata gttggcggtg atgtggttca
     4981 tcacttcacg cggtgaaaac tcacgcggtt cgatcttcaa ctgttccgat tcaatcttcg
     5041 agaaatcgag aatatcgctg ataattttca acaacaggct ggaagagttg ttcattgccg
     5101 tcaccagccg atcgacgcct ttcggtaact ctttggtttg caacagatcc aggttaccga
     5161 taatgccata cagcggcgtt cgcagctcat gactgacggt ggcaaggaac atcgattttg
     5221 actggctcgc ctgttccgct gcttgtgcca tctcctgcaa cgactcttcc atcttcacgc
     5281 gcgaagaaac atccaccagc acacaaatgg ccacgttttc attacgatag cgcgaatgga
     5341 cgaagctgat ttgcagattg gtattgttgc tggtcaggac atcaacaaaa ttgacctgct
     5401 gcccacagat aatttgcgtc agtcgttggc ggtcctcatg cgtaagcata ttgagatagg
     5461 tatgcgccag ttcgttactt aaaatattga cgccatcagc ggtacgcaaa atgcagatac
     5521 ccactggcgc ggaggcgaca atcttgcgat tgaactgctc atgttcttcc agtcgcaggg
     5581 cgtcgctttc cgccggaatg aaaatacgtc gctcgtacat ccgcgcgaga gtaaacaatg
     5641 cagctccggc aagcacattc agcaaaattg cgttaaggat caacatgcga atgcgttcca
     5701 gcaccttatc aaccggcacc gaatacacga tgcttagcga tgagggtggc agatttttct
     5761 tcagcaccag ctcccggaac ccttccgtat agccaaacca ggagcgttcc tgcatccagc
     5821 gaggatcgcc cttaatttta ctttctggtc cggtaagcga aatcagggta tgaccatttt
     5881 catcaagaat ggtaaccccc atcggcaacg tacccggtaa gaaaaagttc tccatccgga
     5941 tggtctgctc gacacccaaa agcgcctgca accggttcgc cagataaact ggcgtcaacg
     6001 cgtaaaaata cccgacgcca gggcgcggac cttcgctgat ccagtagagg ttactgccgc
     6061 tatcatcttg tggtgcattt cgatatttat tgatgcgttc atgcaaagct ttcaacgcgg
     6121 tatcgcgttc cactggcata tcacgcagac cgaaattggc catgcagagg ttatcactgc
     6181 cgattaaaaa tacccggttg agatcgtaag ccgcagaaaa attatcgcgc cagtagcgca
     6241 taaaccacgc caatgactcc agagaacctc gccaggtgtt actcattgcg gaacaatcgg
     6301 agtcggcaaa cagcggttca aacgcaggca catccgcctg cgtttctcgt ccacgcgggg
     6361 aaagcacacc gttttctgcc gataagcgat tttcggcgat gtacttcagc tctttcatca
     6421 catcagaggt gcgttgaata aagcgctgag cctgatcgga actcagatta aattcctgac
     6481 gaatttccga ttctcgctga tgtaacgcat taacgatgta aaaaacggat gaaaaagcaa
     6541 tcaacagcca gagcactaac gccaatgctc tgaacatgta gcgcgaggct ttcagggttg
     6601 tacgaaaaga agcaaggtat ttcaaagggg cgaagctccg cctcaggtga ccgatggagt
     6661 gtggttaagg tagcggtaaa agcgtgttac cgcaatgttc tgtcaccatt gcaataatgc
     6721 gtatcgctta gaaaccattg ccttaaccag gaaagccgga gtagatggac agttctcagt
     6781 acgaaaaata agaaatgacc aatctgccga cgcttgttaa tggttatatc ctggcgtcgg
     6841 cagatcgccc agcagtctta tctgaaaaaa gagagagcca ggttgtgtga agggatggat
     6901 caccagtcaa aacttttcac cagcgtcagc tcgccagcat tacgcatcgg tacaataaat
     6961 gtttcctgtt tctcattgac cgatccttca tcggtgatca gcgtcagttg ggcggtcgtc
     7021 aattccgttt cgctgcgccc accatagtag ttgatataca cctgatagcg cccgtgaact
     7081 ggtgctggca tggcgaaaat ctcgggtccg taccccgtcg tgacatccat atccagtgca
     7141 ccactgtttt tcagcacggt gttaccgtac caggcgtgtt cgccatcggg cgtgacaaca
     7201 tggagatcga gatcggtatt gtccgtatcc cacgagagaa ccagccgtaa acgtgcacga
     7261 atcgttcctg tacccggcgt tgagtaaaac tgcatttttt gtcggctttg cccatccggg
     7321 ctgatgacct gcacgctgtt gctgccttca gtgaaaatat aagggcgtgc aaatgaacca
     7381 tcggattcaa ctctctgcgg catactggca ccgttgacca ccagccggcc ctgctgaact
     7441 ttacccgccg cagcataatt ctttattttg ccgcggatct gcgctgagat attttgatca
     7501 tctgccatgt tgacggacga cgccgggtaa ttgatcgatt gtgaaaaact ggcatcttcg
     7561 ccctctgccg gatgccagcc agagagcggt gcgtcaatct cgacttcctg cacaccttca
     7621 ctgtgagcga caggcgaaag tgccaccagt aacaacggaa gaaaaatctt tcgcatagca
     7681 ttagtccatt aataattgtc gggtcagcgt ttcgatatag ttctcatcaa gcccggtggg
     7741 ataaccatca aaggccaggt gcagatactc atgggttaaa tccagccgat cctgaagcgt
     7801 aaagaagtta cgaataaaca gccgtttctg ctgccgatcg gtatagggga aacctgagac
     7861 taaacgacag accgcaaaca cgtctggttc gttgtacccc gtctcgcctt gtaatatacg
     7921 ccgccactgc ggcatttttt tcgccagcca ggctttcgct ttgggtaata actggcaggt
     7981 tgagcgtgga gctccccagc ggctaaggct gttgtcggga taagcaaacg cgagtatctg
     8041 gtcgtaacgc tccccctgcc ccgcctgggc cgtggcttgc cgccaggaaa gtgtcccttc
     8101 ggtagcgcga ctgccatgat aatgaaccgg atcgcctgca tagatgagat cctgcgtcca
     8161 ggcggtcatc gttcgcgccc ccgtggtcgc tggcgaagca gaaacacgct gcgtggcgct
     8221 gctgtcgggg atggtcaggc aatcgccttc ccgattggcg ttttgctgta aaaaagtgcg
     8281 aatcgccacg gtcatggctt tcgctgcctc agggggcgta cttttcgcct cgcgatccag
     8341 aactctggca acgtactctt cacgatcgag atgcgactgc aatttcagtt tgcccttttc
     8401 gctcagtaag gtcgtctcac cgtggctgac aaacgtaatg tggttaccat tggtaaacgt
     8461 cacacgatag cgcccattaa gtacgccggg ttttactgcc gtcgtactct tttccgctgt
     8521 gattttcttc agcggatagc gggcaaacag ctccacctct acacattgcc cactggcaac
     8581 ctgcgtcggc actggcaaaa cccgattaag caccgtcgca taacgggtta gtaccgtttt
     8641 gctggtgcca ctgccagtag cccacagcgg tgtaccatcg gttaaccagc cggcaaaacc
     8701 accctgacgg atttcctgtt tatcatcggc aaaccagctc caggttttca cccgtacccg
     8761 actaccgagc atggatgcga ctccgatttt cgcctcgtcc agcaccacat ccagtaagac
     8821 ttcctgagct ttgttttgcg caggtaacgt cgctaatgaa tcaagcagcg atttaaccgt
     8881 taccgaggtt tctggcttca gcgtagtaag agacgctaac cacgcaggtg cctgacgctg
     8941 ttgccagtat tgcccccaca catctgcgcc aatatgcaac cgctgcggcg caaaatagag
     9001 cccacaggaa cgtaccagcg cggtatcgcg cgttatgctc tccccagcct gacaacaata
     9061 aacctcttcc ggggagtttc cgcgacactg gtagggctgc tccggttgat gggtatcctc
     9121 cagccaggcg taaacataca gtttccacaa actccctaac ggcgtggtga gcgagtcagg
     9181 taatgtcgag gctttggtca cgcctgatga cgacagctga tacagttgat cgtgctgcgc
     9241 accgcgcagc accagttgta acggcgtctc ctccgcaagc gttggcagag taaccaacgc
     9301 caatagccag acgattctgc gccagttcac ttatttcacc tgcatcccgg tccattcact
     9361 ccctgccgca acactttgct gcgcaggtgc ataggaacgc acataacgag caggaggcag
     9421 aacgaattgc cctttttgcg agaagcgcag caaatggcgg aaagtgaccg ttcccgtcag
     9481 ttctttcacc ggcaccatat aggccagttc gcccatttca ttacgcgctt tttccagcaa
     9541 ttgcccctgc tgtttcgcgg cgttgggttt attgactgaa atgccccatg ttgtgcgctc
     9601 aacgtcggct cccggcggga gcggtacttc cacctgacca tagcgcagaa ctgcatcctg
     9661 ctcgctggta agcgtaattt catcgagata cagcgcatcg ctgtcaatct cattgctggt
     9721 caccggttgc agaataaagc tcatctcttc ttcaccaggg ataagccgat acaactggcg
     9781 ttcaacggtc accggaatgt tactttgttg agccattttt gccggctcac gccagcggac
     9841 ctgcacattt tgcggcgata attcgtcacc aaaagagaga atgtccggca cgccctgacc
     9901 aacccaacgc cagtcttcgc cccctccagt taacttatgt ttagcccatg cgcccgcagg
     9961 cgcaggcaaa acaactggag gcatcgtcgc catatatttc gccagccagt tcatggccag
    10021 cgcgcgctca atagtggatt gctcagcggt caaaccactt aaaatagcag ccgcctgggt
    10081 agcgtcgcca ccgctattaa gcagcacaac cgtgtgcgcc agcggctgat tgctgtttgc
    10141 cgctttatcc cacgcatatt gctgaacctg tggcatcacc gtggacttca actgcgcttt
    10201 tttcagcaac gtcatggtta agacgtttgc caccgcatct gccagtggag attccggtgt
    10261 atcgaggatc aggctgtcat tgatatcgcg ggtgtcttcc tcagagaaat cttcatcttt
    10321 agttccgcgc cgggcgatag cttcatccaa ccctttcaac aacgttttgc acggcagatt
    10381 catctcctgt gcccacgcca gcaccagcgc ccgatgcaat aacggcatgt tatctgcctg
    10441 ctcggcataa ctgtcgagca tatgctgcca gtattccggt tgttgcgtta cgccgagcgc
    10501 ctggctggcc tgccagtcgg cgtaccatgc ccatgccgta aggaaggcgt caccattgcc
    10561 atcttcaccc caccaggtaa agcgcgctcc gggccccgcc agttgcatca gccgcagacg
    10621 gttatcctga atcatctgac gaatgtcgtt agcggcggca ctttgatgat cggcaagcga
    10681 acgccaggcg agactgagcg ggatcagacg gctaccggtg ttgatcaccc caccccacgg
    10741 ttcatcgact aacgcatcaa gattgttgcg gaaaatctcc tgcggcgttt cactactttg
    10801 tagccggata ttgctcgcct gctcgggcaa catcagcgcg ttctcgccac cgccgagcat
    10861 gacatttttc tgctgttcaa cgggccagct gttgtccaca aaagacagtt ttgtgctaat
    10921 gctgtcctgc acttgcccat tttgttgcag ttctgcactt aacaagccag attgctgaat
    10981 attctgcgtc agcgaaatat aattcgcccc tttgtgcagc gtcagcgtct ggcgcatctc
    11041 agcgcctgca aatttcgtca ccagcgccac cggttcgtta tcctgctgac tgaagataaa
    11101 cagtcctgct gccggtttgt cgcctatgcg atacaccgtt ggcatactcc acttcatgta
    11161 gagatttttt tccgaacgca gataagcacg cccctgcccg accagcccgt cgccgttcat
    11221 cccacgcgcg gtgatacgcc agcgggttaa cgaatcaggc atcaggaacg tgaaatacgc
    11281 tttgccttgt ttatcggttg tgagtgacgg catccatgcc gcggtatcca cctcttcacg
    11341 ccgtggacgt tcaagcattt ttactcgccg ctcgctgcgg ttagttgcgc caggcgcaac
    11401 cggctcgctg gagagcgcct ggtcgtagct gataaacgac agactggagc tggtacgcac
    11461 attgttacgc cccagcggat agaaaaattt gccgatattc ggcgcgattt ctggttgcag
    11521 cgcgtagatc atttcatcga ccacgcctac cgttagctgc gcagaaacag gtttaccttt
    11581 cagcgacgag gttaattcga cattgaccag ttcaccaggc tggtaatggg ttttgtccgt
    11641 tttcacccgg atatcaagct gaggaacggc aactttgatc ccggcgttct gaaaactgta
    11701 ctgaccatta cgggtataca gcaccgaaaa agtgatgtta ggcgcaaagg aattgctcac
    11761 tggaacccgg gcttcatact gggtatcgtt taaacgttgt aacgtcagcc agtttgccgg
    11821 atgcgaaagc agcgactgct gttccacgcg atcgcgttcc agcgtcaata atgcttcatc
    11881 aattggctcc ggaaaggtaa tcagcatctt cgcagtttcg cctggctggt acagcgtttt
    11941 atccgccacg atatctaccg taccagtatg cgccgtgctg cccttaccgc tgacggcatg
    12001 acttaaccca gcgagaatta agccgtcttt atcgcgtaat gtcagattgt agttgccagg
    12061 tttagcgaaa ttgacggtaa aggatttgcc gcctgacggt agctctccgc tatggctcgt
    12121 gcggtcttcg agacgcaacc attcatacgt aacaggaacc tgttttgaag attccagcgc
    12181 ggcataacgg aacacaaccg actcgccgct attactgtat tgtgcggcag tacttaatga
    12241 gtaatgcgcc agaccgcgtt caatgaggat ctctttggtg gtggtgacac gatacgccgc
    12301 gccgtcactg gcggagacgg ttaacaaata acggctcggt ttatcggcgg cggggagatt
    12361 taacgccaca tgaccgctgg cgtcggacac cgtttcgctg ccttccagcg acacggggaa
    12421 acgtccggca taacgcaaat cgttaccgac cattgataat tgctgagcgc gcaaacttaa
    12481 ctgcacgcgg gcatttttta ccggctcgcc atccgggtag agcagttgca gtttgccgct
    12541 gaccgcttcg ccagttttga actctttttt gtcgagagct aaaccaatct cgaaatgtgg
    12601 cttgatgtag tttgccacgc gaaaactgct gctatagacc tgattgcggt aagcaagacg
    12661 taactcataa cctccggcaa cggcattttc tggcaggcgg aaacttccct gcccgccatt
    12721 gcgcgcatcc agcgtgacat tgacggtttg caacagactg ccgttggcgt ccagcaccga
    12781 aagcttcgcc ggggcgctga cgatggggga tgaatgcaac ggatcgtgga actcgcggcc
    12841 catcacttta acatcgacac gatcgcctgc gcgatatagc gggcgatcgg taaagatata
    12901 caagcgggtg ttgtagattt cactttcgta aaagaagttc tcggagacaa aaacgccgcc
    12961 ttcagcatcc ttacccagaa tgtatgagcg ttctggcgat atatgttgta actgcaaggt
    13021 accgctgtca tcggtcacac cgcgggtcat cacgccaaga ccgtcagtcc acaagatctc
    13081 tgagccgggc ttcgcttcac cctgtttttt acccgcggtc cacaccagaa gctcgttgcc
    13141 tgacactttg ctaagcgcca cggtatcgga aacaaacacc accgtcgtcg cccgataccc
    13201 accaaccatc gcctcgacga ggtacagtcc cggctcttgt tggccgagag gaatataaat
    13261 gttacccggc tgcggcgaga tgaaattgct ggatgcgcct tccagtttta ccccttgctg
    13321 cggctcgacc ggtttagcct gccatagtgg ataacggaac tgttccacca agggatattt
    13381 tttcagcggg gaaaactggt tgttctgtac ataacgggaa ggtttaataa tggcattgcc
    13441 gagatgtaat tcgggtaatg cctgagtcac attctgccgt gactgagaag agaaagtacg
    13501 ctgcattacg cggcgagatt tgccgtacca gttatcccac agccaggtca gcgtattgtt
    13561 cagcccgtcg cccagatatt gcggttgcac cacaatgcga tgcaggtttt tctgctggcg
    13621 caaaaatgcc atcgggtcag gaatacgata caggcgaacg tccacgccgc cgtactcttc
    13681 catctgatag cgccgataat cacgccccgg cgcttccagt cgcactttcg cctcttcact
    13741 gctgctaaaa ctgctgtcag caagcaaaaa gaatgttccc ccggcgggcg gcgcatagtt
    13801 actggaagga agcgaatcat cagcattagc aagccctgtt cccacaagag acagggacaa
    13861 gcacgctgct atcgcgccag aaaatttaaa cgataaatgc caatgaaatt gggattggaa
    13921 tcgttgggta tccatcgggt gtccttccat gtcataagtt gttgcagact gactgcgcgc
    13981 attccgttgt cagttttcgt ggcgcttccg gtgtggtaga tgacgtaacg ccccatccag
    14041 accattaagt gctgggcatc gccctgatcg aaaaaaatca tatcgccagg cagcgcctgg
    14101 tttatgtcct agccaataaa ctggctgttg tactgaatca aattaatcgc ggtcacgtag
    14161 gggccggttt tcccgttccc ctgattccag ttttgcgcca gttgacgctg tccaggtgtt
    14221 agcgtcatct ctggcggcaa atactggcta gagaaaccgt tactttttag ccatttacta
    14281 tcgtgaactt tcagcgtctc gttcgccgca aatcgcacca ggcccgcaca atcctgctga
    14341 taccagcgtg gacttggccc ctggcggagc tgttcttgtg caatgcgtac aaaccaggcg
    14401 cgaaacagcc cggattgttc gacattcagc atttcgctat gggcaacaac acaacacagc
    14461 cagcaaatca gcgccagcag cccgtgcctc atagcggctg ccaggttatg ggtaaccact
    14521 gccaggcggc accgggttcc atctgggcca gcttcatgac ataacgcggt tgttgagata
    14581 aagcgtccag cttcggcatt aataaagttt gtgcggcgtt ataaaaaacc ggttcgagat
    14641 tcttcggcag actggtcaac gtttcgttac gcagcagttt cgccacgcct tgtggattga
    14701 tatagagcgg aacgatgcca tcagtgggta ttacatccac cattgccggg cgggttttat
    14761 tcagtgtttg cagcgcgtta ttaaccagcg tgtcatcgag ggagaaaagc agcgttttgt
    14821 tttgcatcgc cagcgacacg cggaaaaaat aatccgacat taattgatcg ggttgcgccg
    14881 cctgcgcttt cggatactgg ccgtatcggg aactcacttc gcgacgccag atttgccctt
    14941 cgccctgctg agtctggctt accggcaaaa caccttctgg agatttaaca caccttactc
    15001 cccttgatga tttacttatt tttcaatgac taaatcaaaa
//
LOCUS       DAZFQD010000128        14962 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.128, whole genome shotgun sequence.
ACCESSION   DAZFQD010000128 DAZFQD010000000
VERSION     DAZFQD010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14962
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.128"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            89..625
                     /gene="yehD"
                     /locus_tag="ACHKTW_003977"
     CDS             89..625
                     /gene="yehD"
                     /locus_tag="ACHKTW_003977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO6464903.1"
                     /translation="MKRSIIAAAVFSYFFMSAGVFAADVDTGTLTIKGNIAESPCKFE
                     AGGDSVSINMPTVPTTVFEGKAKYSTYDDAVGVTSSMLKISCPKEVAGVKLSLITNDK
                     ITGNDKAIASSNDTVGYYLYLGDNSDVLDVSAPFNIESYKTAEGQYAIPFKAKYLKLT
                     DNSVQSGDVLSSLVMRVA"
     gene            712..1386
                     /gene="yehC"
                     /locus_tag="ACHKTW_003978"
     CDS             712..1386
                     /gene="yehC"
                     /locus_tag="ACHKTW_003978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFO6464904.1"
                     /translation="MKGLLSLLIFSMVLPAHAGIVIYGTRIIYPAENKEVMVQLMNQG
                     NRSSLLQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKES
                     IFYLNVLDTPPNSPEQEGKNALKFAMQNRIKLFYRPAGIAPVNKATFKKLLVNRSGNG
                     LVIKNDSANWVTISDVKANNVKVNYETIMIAPLESQSVNVKSNNANNWYLTIIDDHGN
                     YISDKI"
     gene            1402..3882
                     /gene="yehB"
                     /locus_tag="ACHKTW_003979"
     CDS             1402..3882
                     /gene="yehB"
                     /locus_tag="ACHKTW_003979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HFO6464905.1"
                     /translation="MLRMTPLASAIVALLLGIEAYAAEETFDTHFMIGGMKDQQVANI
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPQETCLSREVIKRLGINSDNFASGK
                     QCLTFEQLVQGGSYTWDIGVFRLDFSVPQAWVEELESGYVPPENWERGINAFYTSYYV
                     SQYYSDYKASGNSKSTYVRFNSGLNLLGWQLHSDASFSKTNNNPGVWKSNTLYLERGF
                     AQLLGTLRVGDMYTSSDIFDSVRFRGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVT
                     IEQNGFVVYQKEVPPGPFAITDLQLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQ
                     PGVSKYDFAAGRSHIEGASKQSDFVQAGYQYGFNNLLTLYGGSMVANNYYAFTLGTGW
                     NTRIGAISVDATKSHSKQDNGDVFDGQSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRT
                     FNDHVWANNKDNYRRDENDVYDIADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLW
                     RDYWGRSGSSKDYQLSYSNNWRRISYILAASQTYDENHHEEKRFNIFISIPFDWGDDV
                     TTPRRQIYMSNSTTFDDQGFASNNTGLSGTVGSRDQFNYGVNLSYQNQGNETTAGANL
                     TWNAPVATVNGSYSQSSTYRQAGASVSGGIVAWSGGVNLANRLSETFAVMNAPGIKDA
                     YVNGQKYRTTNRNGVVVYDGMTPYRENHLMLDVSQSDSEAELRGNRKIAAPYRGAVVL
                     VNFDTDQRKPWFIKALRADGQPLTFGYEVNDIHGHNIGVVGQGSQLFIRTNEIPPSVN
                     VAIDKQQGLSCTITFGKEIDESRNYICQ"
     gene            3896..4930
                     /gene="yehA"
                     /locus_tag="ACHKTW_003980"
     CDS             3896..4930
                     /gene="yehA"
                     /locus_tag="ACHKTW_003980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
                     /protein_id="HFO6464906.1"
                     /translation="MNIRIMLFIVMMMVMPVSYAACYSELSVQHNLVVQGDFALTQTQ
                     MATYEHNFNDSSCVSTNTITPMSPSDIIVGLYNDTIKLNLHFEWTNKNNITLSNNQTS
                     FTSGYSVTVTPAASNAKVNVSAGGGGSVMINGVATLSSASSSTRGSAAVQFLLCLLGG
                     KSWDACVNSYRNALAQNAGVYSFNLTLSYNPITTTCKPDDLLITLDSIPVSQLPATGN
                     KATINSKQGDIILRCKNLLGQQNQTSRKMQVYLSSSDLLTNSNTILKGAEDNGVGFIL
                     ESNGSSVTLLNITNSSKGYTNLKEIAAKSKLTDTTVSIPITASYYVYDTNKVKSGALE
                     ATALINVKYD"
     gene            complement(5012..5350)
                     /gene="rcnB"
                     /locus_tag="ACHKTW_003981"
     CDS             complement(5012..5350)
                     /gene="rcnB"
                     /locus_tag="ACHKTW_003981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HFO6464907.1"
                     /translation="MTIKNKMLLGALLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(5569..6393)
                     /gene="rcnA"
                     /locus_tag="ACHKTW_003982"
     CDS             complement(5569..6393)
                     /gene="rcnA"
                     /locus_tag="ACHKTW_003982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
                     /protein_id="HFO6464908.1"
                     /translation="MTEFTTLLQQGNAWFFIPSAILLGALHGLEPGHSKTMMAAFIIA
                     IKGTIKQAVMLGLAATISHTAVVWLIAFGGMVISKRFTAQSAEPWLQLISAVIIIGTA
                     FWMFWRTWRGERNWLENMHEYDHEHHHHDHEDHHDHGHHHHHEHGEYQDAHARAHAND
                     IKRRFDGREVTNWQILLFGLTGGLIPCPAAITVLLICIQLKALTLGATLVVSFSIGLA
                     LTLITVGVGAAISVQQVAKRWSGFNTLAKRAPYFSSLLIGLVGVYMGVHGFMGIMR"
     gene            6514..6786
                     /gene="rcnR"
                     /locus_tag="ACHKTW_003983"
     CDS             6514..6786
                     /gene="rcnR"
                     /locus_tag="ACHKTW_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HFO6464909.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     regulatory      6878..6974
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            7009..7797
                     /gene="thiM"
                     /locus_tag="ACHKTW_003984"
     CDS             7009..7797
                     /gene="thiM"
                     /locus_tag="ACHKTW_003984"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HFO6464910.1"
                     /translation="MQVDLLSSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMRAAVEQAKSSQTPWTLDPVA
                     VGALDYRRHFCHEFLSFKPTAIRGNASEIMALAGVANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEVDYVTDGHRIVGIHGGDPLMTKVVGTGCALSAVVAACCALPGDML
                     ENVASACHWMKQAGERAVARSEGPGSFVPHFLDALWQLTQEVQA"
     gene            7794..8594
                     /gene="thiD"
                     /locus_tag="ACHKTW_003985"
     CDS             7794..8594
                     /gene="thiD"
                     /locus_tag="ACHKTW_003985"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFO6464911.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKSWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            8659..9477
                     /gene="yegX"
                     /locus_tag="ACHKTW_003986"
     CDS             8659..9477
                     /gene="yegX"
                     /locus_tag="ACHKTW_003986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HFO6464912.1"
                     /translation="MQLRITSRKKLTSLLCALGLISIVAIYPRQTVNFFYSTAVQITD
                     YIHFYGYRPVKSFAIRIPASYTIHGIDVSRWQERIDWQRVAKMRDNGIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVAAPVQARLFLQTVDFLQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKSTGKKPIIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFHGTVEELQAFVDGIKETP"
     gene            9529..10275
                     /gene="yegW"
                     /locus_tag="ACHKTW_003987"
     CDS             9529..10275
                     /gene="yegW"
                     /locus_tag="ACHKTW_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFO6464913.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(10249..11214)
                     /gene="yegV"
                     /locus_tag="ACHKTW_003988"
     CDS             complement(10249..11214)
                     /gene="yegV"
                     /locus_tag="ACHKTW_003988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HFO6464914.1"
                     /translation="MSGARLHTLLPELTSRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     GDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGSLLYFSGYQLASPCGEL
                     LVEWLEKLQDVTPFIDFGPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGKQWQEKFAAPLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(11211..12215)
                     /gene="yegU"
                     /locus_tag="ACHKTW_003989"
     CDS             complement(11211..12215)
                     /gene="yegU"
                     /locus_tag="ACHKTW_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HFO6464915.1"
                     /translation="MKTERILGALYGQALGDAMGMPSELWPRSRVKAHFGWIDRFLPG
                     PKENNAACYFNRAEFTDDTSMALCLADALLEREGKIDPDLIGRNILDWALRFDAFNKN
                     VLGPTSKIALNAIRDGKPVAELENNGVTNGAAMRVSPLGCLLPARDVDSFIDDVALAS
                     SPTHKSDLAVAGAVVIAWAISRAIDGESWSAIVDSLPSIARHAQQKRITTFSASLAAR
                     LEIALKIVRNADGTESASEQLYQVVGAGTSTIESVPCAIALVELAQTDPNRCAVLCAN
                     LGGDTDTIGAMATAICGALHGVNAIDPALKAELDAVNQLDFNRYATALAKYRQQREAV
                     "
     gene            complement(12212..13489)
                     /gene="yegT"
                     /locus_tag="ACHKTW_003990"
     CDS             complement(12212..13489)
                     /gene="yegT"
                     /locus_tag="ACHKTW_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015858 - nucleoside transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease"
                     /protein_id="HFO6464916.1"
                     /translation="MKTTAKLSFMMFVEWFIWGAWFVPLWLWLSKSGFSAGEIGWSYA
                     CTAIAAILSPILVGSITDRFFSAQKVLAVLMFAGALLMYFAAQQTTFAGFFPLLLAYS
                     LTYMPTIALTNSIAFANVPDVERDFPRIRVMGTIGWIASGLACGFLPQMLGYADISPT
                     NIPLLITAGSSALLGVFAFFLPDTPPKSTGKMDIKVMLGLDALILLRDKNFLVFFFCS
                     FLFAMPLAFYYIFANGYLTEVGMKNATGWMTLGQFSEIFFMLALPFFTKRFGIKKVLL
                     LGLVTAAIRYGFFIYGSADEYFTYALLFLGILLHGVSYDFYYVTAYIYVDKKAPVHMR
                     TAAQGLITLCCQGFGSLLGYRLGGVMMEKMFAYPEPVNGLTFNWSGMWTFGAVMIAII
                     AVLFMIFFRESDNEITAIKVDDRDIALTQGEVK"
     gene            13746..14798
                     /gene="fbaB"
                     /locus_tag="ACHKTW_003991"
     CDS             13746..14798
                     /gene="fbaB"
                     /locus_tag="ACHKTW_003991"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HFO6464917.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLASVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
BASE COUNT         4024 a         3489 c         3764 g         3685 t
ORIGIN      
        1 ttgcggcaac ttttttgatg tgagaaattt gtttccaaga gatacagact cttaacaacc
       61 aaaaataaat tttattaagg aacatattat gaaacgttca attattgctg ccgctgtctt
      121 ttcttatttt tttatgagcg ctggagtatt tgctgcagac gttgataccg gaacattaac
      181 tattaagggg aatattgcag aatctccgtg taaattcgaa gcgggtggtg attcagtaag
      241 tattaatatg ccgactgtac caaccactgt ctttgaaggt aaagctaaat attctaccta
      301 tgatgatgca gtcggtgtaa ccagcagcat gttaaaaatt agctgcccga aagaagttgc
      361 tggtgtaaaa ctctcgttga ttaccaacga taaaataacc ggtaacgata aggcgatagc
      421 cagtagcaac gataccgtgg gttactatct ctatttaggt gataacagcg atgtcctgga
      481 tgtttctgca ccttttaaca ttgagagtta taaaacagcg gaaggtcaat atgctattcc
      541 gtttaaagca aaatacctga aactgacaga taactcagtg caatcaggtg atgtgttatc
      601 ttctctggtt atgcgtgtgg cgtaggatta ataaataaaa ttgtaagttt ctccaggtaa
      661 taaacatggc cgctatcccc atggcggctt tttaatttaa gaggcattaa gatgaaagga
      721 ttattatctt tactcatttt ttctatggtc cttcctgcac atgccggaat tgttatctac
      781 gggacgcgca ttatttaccc ggcagaaaat aaagaagtta tggtgcagtt gatgaaccag
      841 ggaaaccgtt cttcgctgct gcaggcgtgg attgatgatg gcgatacgtc gttaccacca
      901 gaaaaaattc aggttccttt catgttaacg ccaccagtgg caaaaatagg ggcaaattcc
      961 gggcagcaag taaaaatcaa aattatgccg aataaactgc ccactaataa agaaagcatt
     1021 ttttatctga atgttctgga tactccacca aatagtccag agcaagaagg caaaaatgca
     1081 ctgaagtttg cgatgcaaaa cagaattaag ttgttttacc ggccagcggg tattgctccg
     1141 gtaaataaag cgacctttaa aaagttactg gtaaatcgca gtggcaatgg attggtgata
     1201 aaaaatgact cagctaattg ggtgacgatt tcggatgtca aagctaataa tgttaaagtc
     1261 aattatgaaa ctataatgat tgccccctta gaaagtcaga gtgttaatgt caaaagtaat
     1321 aatgcaaata actggtatct gaccattatc gatgaccacg gcaactatat tagtgacaaa
     1381 atttaattgc aggagctgcc tatgttgaga atgaccccac ttgcatcagc aatcgtagcg
     1441 ttattgctcg gcattgaagc ttatgcagct gaagaaacct ttgataccca ttttatgata
     1501 ggtggaatga aagaccagca ggttgcaaat attcgtcttg atgataatca acccttaccg
     1561 gggcagtatg acattgatat ttatgtcaat aagcaatggc gcgggaaata tgagattatt
     1621 gttaaagaca acccgcaaga aacatgttta tcaagagaag ttatcaagcg gttaggcatt
     1681 aatagcgata acttcgccag cggtaagcaa tgtttaacat ttgagcaact tgttcagggt
     1741 gggagctata cctgggatat cggggttttt cgtctcgatt tcagtgtccc gcaggcctgg
     1801 gtggaagaac tggaaagtgg ctatgttcca ccggaaaact gggagcgggg tattaatgcg
     1861 ttttatacct cttattatgt gagtcagtat tacagtgact ataaagcgtc gggtaatagt
     1921 aagagtacat atgtacgttt taacagcggg ttaaatttac tggggtggca actgcattct
     1981 gatgccagtt tcagtaaaac aaataacaat ccaggggtgt ggaaaagcaa taccctgtat
     2041 ctggaacgtg gatttgccca acttctcggc acgcttcgcg tgggtgatat gtacacatca
     2101 agcgatattt ttgattctgt tcgcttcaga ggtgtgcggt tgtttcgtga tatgcagatg
     2161 ctgcctaact cgaaacaaaa ttttacgcca cgggtgcagg ggattgctca gagtaacgcg
     2221 ctggtaacta ttgaacagaa tggttttgtg gtttatcaga aagaggttcc tcctggcccg
     2281 tttgcgatta cagatttgca gttggccggt ggtggagcag atcttgatgt cagcgtgaaa
     2341 gaggcggacg gttcggtaac cacctatctg gtgccttatg cagcggtgcc aaatatgctg
     2401 caacccggcg tgtcgaaata tgattttgcg gcgggtcgta gccatattga aggggcgagc
     2461 aaacaaagtg attttgtcca ggcgggttat cagtatggtt ttaataattt attgacgctg
     2521 tatggtggct cgatggtcgc gaataattat tacgcgttta ctttggggac tggctggaat
     2581 acacgcattg gtgccatttc cgtcgatgcc acgaagtcgc atagtaaaca agacaacggc
     2641 gatgtgtttg acgggcaaag ttatcaaatt gcctacaaca aatttgtgag ccaaacgtcg
     2701 acgcgttttg gtctggcggc ctggcgttat tcatcgcgtg attaccggac atttaatgat
     2761 cacgtttggg caaacaataa agataattat cgccgtgatg aaaacgatgt ctatgacatt
     2821 gccgattatt accagaacga ttttggccgt aaaaatagct tctctgccaa tatgagccag
     2881 tcattgccag aaggctgggg gtctgtgtcc ttaagtacgt tatggcgaga ttactggggg
     2941 cgtagcggca gcagtaagga ttatcagttg agttattcca ataactggcg gcggataagc
     3001 tatatcctcg cggcaagcca gacgtatgac gagaatcatc atgaagagaa acgttttaat
     3061 atttttatat cgattccctt tgattggggt gatgacgtta cgacgcctcg tcggcaaata
     3121 tatatgtcta actcaacgac gtttgatgat caggggtttg cctcaaataa tacgggatta
     3181 tcaggaaccg ttggaagccg ggatcagttt aactatgggg tcaacctgag ttatcagaat
     3241 cagggaaatg aaacgacagc tggggcgaat ttgacctgga acgcgccggt tgcgacagtg
     3301 aatggcagtt atagtcagtc gagtacttat cgacaggctg gagccagtgt ttcagggggc
     3361 attgtcgcct ggtcgggggg cgttaatctg gcgaaccgtc tttccgaaac gtttgctgtg
     3421 atgaatgcgc caggaattaa agatgcttat gtcaatgggc aaaaatatcg cacaacaaac
     3481 cgtaatggag tggtggtata cgacggaatg acaccttatc gggaaaatca cctgatgctg
     3541 gatgtgtcgc aaagcgatag cgaagcagaa ttacgtggca accggaaaat tgccgcacct
     3601 tatcgcggcg cggttgtact ggttaatttt gataccgatc agcgcaagcc ctggtttata
     3661 aaagcgttaa gagcggatgg gcaaccatta acgtttggtt atgaagtcaa tgatatccac
     3721 ggtcataata ttggcgttgt cggccaggga agccagttat ttattcgcac caatgaaata
     3781 ccgccatcgg ttaatgtagc aattgataag caacaaggac tttcatgcac aatcaccttc
     3841 ggtaaagaga ttgatgaaag tagaaactat atttgccagt aatagtcagg tggttatgaa
     3901 tattcgtata atgttattta tagtaatgat gatggttatg cctgtgagct atgcggcatg
     3961 ttatagtgag ttatctgttc agcacaattt ggttgttcag ggggattttg cacttactca
     4021 aacacaaatg gcgacatatg agcataattt taatgattcg tcatgcgtga gtacaaatac
     4081 tatcacccct atgagcccgt cggatattat tgttggactt tataacgata ccattaaatt
     4141 aaatttacat tttgaatgga ccaataaaaa caacatcacg ttatcaaata atcagaccag
     4201 tttcaccagt ggttattcgg taacggtgac acctgcggcc agtaatgcaa aagtgaatgt
     4261 ttctgcgggg ggcggcggtt cagtgatgat taatggagtt gcgacattat ccagtgcttc
     4321 atcatcgaca cgcgggagtg ccgcagtaca atttctactg tgtttattag gtggcaagtc
     4381 atgggatgca tgtgtaaata gctacagaaa tgcattggca caaaatgcag gtgtctattc
     4441 ctttaatctg acattgtcat acaacccgat aaccacaacc tgcaaaccgg acgatttatt
     4501 aattacttta gacagtattc ccgtttcaca attaccagcc acaggtaaca aagcaacaat
     4561 aaatagtaaa caaggggata ttattctgcg ttgtaaaaat ttattaggtc aacaaaatca
     4621 aacatcacgg aaaatgcagg tgtatttatc aagttctgac ttgttaacca acagcaacac
     4681 aatactgaaa ggtgcggaag ataatggcgt aggatttatt cttgaaagta atggttcgtc
     4741 agtcacactt ttaaatatca ctaacagcag taaaggatac acaaatttaa aagaaattgc
     4801 ggcaaagtca aaacttacag atacaacggt ttcaattccg ataactgcca gttactacgt
     4861 ctacgataca aacaaagtta aatctggcgc actggaggca accgcattaa tcaacgtgaa
     4921 atacgactaa taacaagcaa gacgagcaag tggctaataa taaaaaataa caaggtgtct
     4981 gcatttcccc ctcatgatga gggggctttt tttagcgatg ataaaaaatc tcaccgtcgt
     5041 aggctttaat gattttaccg tcggtgtcgc tgatcaacac gtacgcgcca cccatatagg
     5101 tccagtgcgt cccggcatca ggcgcgggca ggttacgtaa ctgccactgc ttaatgttgt
     5161 actcatcggt gcggtacatc tctgggacgg tatcgcctgg tttgaaatgc ttaaagtcag
     5221 caatgaaact gcttaactca tacttagaaa ttcccgaggt attggtcgaa cccgcggtgg
     5281 ctggtgcggc ccaggcggca ctggtaacca gcaaaagcgc acccagcaac atcttatttt
     5341 taatggtcat aaactctcca ctttggggat ctgtaatatc agtcgcatat ctttcctgcc
     5401 atacaatata actgacaagc gatattttgt tagtgatccc atgatttcgt aaaaagtgta
     5461 aatgaaagga tgccctaaaa gcatccttag cgagcagaga gtcagcggaa atagctgatt
     5521 taagaatcat cctgggctga agataacatg caagcctaaa ggattgagtt atcgcattat
     5581 gcccatgaag ccatgtacgc ccatatacac accgactaag ccaatcaaca gactggaaaa
     5641 atagggggcg cgtttagcga gagtgttaaa tccgctccag cgttttgcga cctgctgaac
     5701 gctgattgct gcgccaacgc ctacggtgat aagcgttaac gccaggccaa tgctgaaact
     5761 gacgaccagt gttgcgccca gtgtcagggc tttcaactga atgcatatca acagcacggt
     5821 aattgctgcc gggcagggga taaggccacc ggttaagcca aataacaaaa tttgccagtt
     5881 ggtgacctct ctaccatcaa agcgtcgttt aatgtcattg gcatgggctc gtgcatgggc
     5941 atcctgatac tcgccatgtt cgtgatggtg atgatgtcca tggtcgtggt gatcttcgtg
     6001 atcgtggtga tgatgttcat gatcatactc gtgcatattc tccagccagt tgcgttcgcc
     6061 gcgccaggta cgccagaaca tccagaacgc ggtgccaata atgatcactg cggaaatcag
     6121 ctggagccac ggttctgctg attgagcagt aaagcgcttg ctgatcacca tcccgccaaa
     6181 ggcaattaac cagaccactg cggtatgcga aatagttgct gccagtccga gcatcaccgc
     6241 ttgtttaatg gtgcctttga tggcgatgat aaacgccgcc atcatcgttt ttgagtgccc
     6301 tggttccagg ccatgaagcg caccaagtaa gatggcgctg gggatgaaga accaggcgtt
     6361 tccttgctga agaagagttg taaattcggt catgataata attcttagta ttaattcggc
     6421 aatctgattc tactcccccc cagtacctga tactaccccc cagtagatta atagtgctat
     6481 gatttttcat attcttgtta accaggtgtt gccatgtctc atacaatccg tgataaacag
     6541 aaactgaaag cgcgtgccag taagattcag ggccaggtcg tggcgctcaa gaaaatgctc
     6601 gacgagccgc acgaatgcgc tgcagtttta caacagattg ctgctatccg tggcgcggta
     6661 aacggtctga tgcgggaagt gattaaaggt catctgacgg aacacatcgt tcaccagggg
     6721 gatgagctaa aacgtgaaga agatctggat gtcgttctga aggtgctgga ttcatatatc
     6781 aaataattta ttaacgcgat tgtaaaactg ccgtttttcc tcgtttacaa cgcgtgcgct
     6841 ggacattacc atcctcctct gcgatttatc atcgcaacca aacgactcgg ggtgcccttc
     6901 tgcgtgaagg ctgagaaata cccgtatcac ctgatctgga taatgccagc gtagggaagt
     6961 cacggaccac caggtcattg cttcttcacg ttatggcagg agcaaactat gcaagtcgac
     7021 ctgctgagtt cagcgcaatc tgcgcacgcg ttacaccttt ttcaccaaca ttcccctctt
     7081 gtgcactgca tgaccaacga tgtggtgcaa acctttactg ccaatacctt gctggcgctc
     7141 ggtgcatcgc cagcgatggt tatcgaaacc gaagaggcca gtcagtttgc ggctatcgcc
     7201 agtgccttgt tgattaacgt tggcacactg acgcagccac gcgcacaggc gatgcgtgct
     7261 gccgttgagc aagcaaaaag ctctcaaaca ccctggacgc ttgatccagt agcggttggt
     7321 gcgctcgatt atcgccgcca tttttgtcat gaatttttat cttttaaacc gacagcgata
     7381 cgtggtaatg cttcggaaat catggcatta gctggcgttg ctaatggcgg acggggagtg
     7441 gataccactg acgccgcagc taacgcgata cccgctgcac aaacactggc acgggaaact
     7501 ggcgcaatcg tcgtggtcac aggcgaggtg gattatgtta ccgatgggca tcgtatcgtt
     7561 ggcattcacg gtggcgatcc gctaatgacc aaagtggtag gaactggctg tgcattatcg
     7621 gcggttgttg ctgcctgttg tgccttacca ggcgatatgc tggaaaatgt cgcatctgcc
     7681 tgtcactgga tgaaacaagc cggagagcgc gcagtcgcca gaagcgaggg gccaggcagt
     7741 tttgttccac atttccttga tgcgctctgg caattgacgc aggaggtgca ggcatgaaac
     7801 gaattaacgc tctgacgatt gccggtactg atccgagtgg tggtgcgggg attcaggccg
     7861 atcttaaaac cttctcggca cttggcgctt atggttgctc agttattact gcactggtgg
     7921 cgcaaaatac ccgtggcgta cagtcggtgt atcgcattga gcctgatttt gtcgccgccc
     7981 agctcgattc ggtgttcagc gatgtgcgaa tcgacaccac taaaatcggt atgttggcgg
     8041 aaaccgatat tgttgaagcg gtggcagaac ggttgcaacg ttatcagatc caaaacgtgg
     8101 tactcgacac cgttatgctg gcaaaaagcg gcgacccgct gctttcacct tcggcggttg
     8161 ctacgctgcg cagtcgatta ttgccacagg tttcattaat aacgccaaac ttgcccgaag
     8221 ctgccgcctt gctcgacgcg ccacacgcgc gcaccgaaca ggaaatgctg gaacaagggc
     8281 gatcgctgtt ggcgatgggc tgtggcgcag tgctaatgaa aggtggtcat ctggatgatg
     8341 agcaaagccc ggactggctg tttacccgcg agggtgaaca acggtttacc gcaccgcgca
     8401 ttatgaccaa aaacacccac ggcactggtt gtacgctctc tgcggcgttg gctgcactac
     8461 gcccgcgcca tacaaactgg gctgacaccg tacaggaggc aaaaagctgg ctttcatcag
     8521 cattagccca ggccgacacg ctggaagttg gtcacggtat tggtccggtt caccacttcc
     8581 acgcctggtg gtgagcaccg tactatagct gctttggtta ttgtcagcct ttggcactga
     8641 cattcaggga tgaaaccaat gcagctaaga attaccagtc gaaaaaaact cacctctcta
     8701 ttatgcgctc ttgggctaat ttccatcgtt gcaatttatc cgcgtcagac ggtgaatttt
     8761 ttctattcga cagcagttca gattacagac tacatccatt tctacggtta tcgcccggtt
     8821 aaatctttcg ctattcgcat tcctgccagt tacaccattc acggaataga tgtttctcgc
     8881 tggcaggagc ggatcgactg gcagcgggtg gcaaaaatgc gcgacaacgg tatccgctta
     8941 cagtttgctt ttattaaggc gacggaaggc gaaaagctgg tggacccgta tttttcgcgt
     9001 aactggcaac taagccgcga aaatggcctg ctgcgcgggg cgtatcatta tttctccccg
     9061 tcggtagcag cgccggttca ggcacgatta tttctgcaaa cggtggattt cttacaaggc
     9121 gatttccctg cggtactgga cgtagaagaa cggggaaaat tatcggcaaa agaattacgc
     9181 aaacgagtaa gtcagtggct aaaaatggtc gaaaaaagta cgggaaaaaa gccgattatt
     9241 tactcaggag ccgtttttta tcacaccaat ctggcgggct atttcaatga atatccgtgg
     9301 tgggtggctc actattatca acgtcgtccg gacaatgacg gcatggcctg gcgcttctgg
     9361 cagcattccg accgtggaca ggtagatggc atcaatggtc cggtggattt taatgtattt
     9421 catggcacgg tggaggagtt gcaggcattc gttgatggga ttaaagaaac gccttaaata
     9481 agaagcagtg atatactccc accagactcc tcggcaggaa aaagacaaat ggaacaagcg
     9541 catacccagc tgattgccca actgaacgag cgtattttag cggcggataa cacgccgctt
     9601 tatattaagt ttgccgaaac ggtaaaaaat gccgtgcgca gcggggtgct ggagcatggc
     9661 aatattttgc ccggtgagcg tgatttaagt cagttaaccg gcgtttcgcg cattacggtg
     9721 cgcaaggcga tgcaggcgct ggaagaagag ggtgtggtga cgcgttcgcg cggttacggc
     9781 acgcagatca acaacatctt cgaatactcg ttgaaagaag cgcgaggttt ttctcagcag
     9841 gtggtattgc gcgggaaaaa gcccgatacg ctatgggtta acaagcgtgt cgtgaaatgt
     9901 cccgaagaag tcgcgcagca gctggcggtc gaagcaggaa gtgatgtctt tttgcttaag
     9961 cgtattcgct atgtcgatga agaagcggta tcgattgagg aatcgtgggt tccggcgcat
    10021 ttaattcatg atgttgatgc catcgggatt tcactttatg actatttccg cagccagcac
    10081 atttacccac aacgtacgcg ttcccgcgtt agcgcccgga tgccggatgc cgagtttcag
    10141 tcacatattc agttagatag caaaataccg gtgctggtga tcaagcaagt ggcgcttgac
    10201 caacagcaac ggcctattga gtacagcatt agccactgtc gcagcgatct atacgttttt
    10261 gtgtgcgagg agtagttcct cgcgcgttgg cgcacaatca ccgccccgat gcccgacaac
    10321 ccacgacgcc actgcattgc ccagtaatac ggcatccgcc agtggcagac cagaggccag
    10381 cccggcaagt acgccacctg catgactgtc gcccgcccca atggtgtcta caacttgcgt
    10441 tggaaatgcc ggaatgcagc cagaagcgtc gttgctgaaa taccatgcac cctctttatc
    10501 gagacgaacg atcaacggcg cggcaaattt ctcctgccat tgcttgccaa gtgttgttat
    10561 ctctgcggat aaagcaaaac gttcggcggc aatctcagcc tcttgacgat tgagtgatac
    10621 taaaggtcga caggccatga tccgcgccag taatgcgtcc gggatatcgc cgatacgtgg
    10681 gccaaaatcg ataaacggcg tcacgtcttg cagcttttcc agccattcca ctaacaattc
    10741 gccgcagggc gaggccagtt gataaccgga aaaatagagc aggctgccag gcgcaacggt
    10801 taatcgcgcc agccactggc gattccactg attttccaca ccgctgaatg acataaaagt
    10861 gcgttcgcca tccggctcaa ccagcgccag acaccagccg ttatcgcctt cggcgttatc
    10921 gatcaggctg attaacccct ctttcgccat ccggttgcga ataatctccg cccacacgcc
    10981 ctgaccgagc ggcaaggcat tacccgcttc gatgccgagg cgctttaacg ccacggcaat
    11041 attcagtgcg cagccgccaa cgttaacgct ctgctgcttc agttcgatat cgcacccacg
    11101 ccaggggagg gcataagcgt cggcgatcac atcaatgacc gccgcgccga cgaccatcac
    11161 cggctgacgc gaggttaatt caggcagcag cgtgtgtaat cgagcgccgc tcataccgcc
    11221 tcccgttgtt gacgatattt cgccagcgct gtggcatagc ggttgaaatc aagctgattt
    11281 accgcatcca gttccgcctt taatgcagga tcgatagcgt taacgccatg caacgcgccg
    11341 caaatcgccg tcgccatagc gccgatggtg tctgtgtcgc cgccaaggtt agcgcacagg
    11401 acggcgcagc gattcgggtc ggtttgtgcc agttcaacca gcgcaatggc gcacggaacg
    11461 gactcaatag tgctggtacc tgcgccaacg acctggtaaa gctgttcgct ggcggattcg
    11521 gtgccgtcgg cattgcgcac aattttcagc gcaatctcca gacgtgctgc cagtgaggcg
    11581 ctgaaggtag tgatgcgttt ttgctgtgca tgtcgcgcaa ttgaaggcag ggaatcaaca
    11641 atcgctgacc agctttctcc gtcaatggca cgagaaatcg cccatgcgat gactaccgca
    11701 cctgcaactg ccagatcgga tttatgggtc gggctcgacg ccagcgccac atcatcaata
    11761 aaggaatcaa catcacgcgc cggaagcaaa caacctaatg gcgagacgcg catcgctgcg
    11821 ccgttggtca cgccgttgtt ttccagttca gcaacgggtt taccgtcgcg aatggcgtta
    11881 agcgcaatct tcgaggtcgg acctagtacg tttttgttaa aggcgtcgaa acgcagcgcc
    11941 cagtcgagaa tattacgccc aatcagatcc ggatcgatct tgccttcacg ttccagtaac
    12001 gcatccgcca gacacagcgc catcgaggta tcgtcggtga attcggcgcg gttaaaataa
    12061 caggccgcgt tattctcctt tggtccaggc agaaaacggt caatccagcc aaagtgtgct
    12121 ttaacgcggc tgcgtggcca aagctcggag ggcatcccca tcgcatcccc taacgcctgc
    12181 ccataaagag caccgagaat acgttctgtt ttcatttaac ttccccttgt gtcaacgcaa
    12241 tatcgcgatc atcgaccttg atagccgtaa tttcgttgtc ggattcgcgg aaaaaaatca
    12301 tgaacagcac ggcgataatg gcaatcatca ccgcgccgaa agtccacatc ccggaccagt
    12361 tgaaagtcag cccgtttacc ggttccggat aagcgaacat cttttccatc atcacaccgc
    12421 caagacgata gccgagcaaa ctgccgaagc cctggcagca gagcgtgatc agcccctgcg
    12481 cggcggtacg catatgcacg ggggcttttt tatcgacata gatgtaagcg gtaacgtagt
    12541 aaaaatcgta acttacgccg tgaagcaaaa taccgaggaa cagtaacgcg taggtgaaat
    12601 attcatccgc actaccgtaa ataaagaagc catagcggat cgcagcggtg accagaccaa
    12661 gcaataatac ctttttgata ccaaagcgtt tagtgaaaaa cggcaatgcc agcataaaga
    12721 atatttcaga gaactggccg agcgtcatcc agccggtggc gtttttcatg ccaacttcgg
    12781 tcagataacc gttggcaaag atgtaataga acgccagtgg catcgcaaac agaaatgaac
    12841 agaagaaaaa gacgaggaag tttttatcgc gcagcaggat cagcgcatcc aggccgagca
    12901 tgactttaat gtccattttg ccggtgcttt ttggcggcgt gtcgggcagg aaaaacgcaa
    12961 acacaccgag cagagcagaa cttccggcgg taatcagcag cgggatgtta gtcggtgaga
    13021 tatcggcata ccccagcatt tgcggcaaga aaccacatgc cagaccagag gcaatccagc
    13081 cgatagtgcc catcacacga atgcgcggga aatcacgctc cacatccggc acgttggcaa
    13141 aagcgatact gttagtcagc gcaatggtcg gcatataggt tagcgagtag gccagcagta
    13201 acgggaagaa cccggcaaaa gtggtctgtt gcgcagcgaa atacatcagc agcgcgcctg
    13261 cgaacatcaa taccgccagc actttttgcg ccgagaaaaa gcggtcagtg atggagccaa
    13321 ccagaattgg cgacaggatc gccgcaatgg ctgtgcaggc atacgaccag ccaatttctc
    13381 cggcactaaa accgctttta cttaaccaca accacaatgg cacaaaccac gcgccccaga
    13441 taaaccattc aacaaacatc atgaacgaca gctttgctgt tgttttcatc ttctaattcc
    13501 ttcatgacag gtaatgtaag gtacgcgatg acaataccac ttaataatac cttttaaata
    13561 cctttgcggt gaaaacttga ccgcgctaac attttttctg atgaatcgag ccaacagaaa
    13621 acgctgaaaa aacatccaaa agatggaaaa actcgcccgt aacgaccagg attatgattg
    13681 cccgcaaaag gcgggccagg acaatccata gccgatatcc aatcggaatt tacgggagca
    13741 tagtaatgac agatattgcg cagttgcttg gcaaagacgc cgacaacctt ttacagcacc
    13801 gttgtatgac aattccttct gaccagcttt atctccccgg acatgactac gtagaccgcg
    13861 taatgattga caataatcgc ccgccagcgg tgttacgtaa tatgcagacg ttgtacaaca
    13921 ccgggcgtct ggctggcaca ggatatcttt ctattctgcc ggttgaccag ggcgttgagc
    13981 actctgccgg agcttcattt gctgctaacc cgctctactt tgacccgaaa aacattgttg
    14041 aactggcgat cgaagcgggc tgtaactgtg tggcgtcaac ttacggcgtg ctggcgtcgg
    14101 tatcgcggcg ttatgcgcat cgcattccat tcctcgtcaa acttaatcac aacgagacgc
    14161 taagttaccc gaatacctac gatcaaacgc tgtatgccag cgtggagcag gcgttcaaca
    14221 tgggcgcggt tgcggttggt gcgactatct attttggctc ggaagagtca cgtcgccaga
    14281 ttgaagaaat ttctgcggct tttgaacgtg cgcacgagct gggtatggtg acagtgctgt
    14341 gggcctattt gcgtaactcc gcctttaaga aagatggcgt tgattaccat gtttccgccg
    14401 acctgaccgg tcaggcaaac catctggcgg caaccatcgg tgcagatatc gtcaaacaaa
    14461 aaatggcgga aaataacggc ggctataaag caattaatta cggttacacc gacgatcgtg
    14521 tttacagcaa attgaccagc gaaaacccga ttgatctggt gcgttatcag ttagctaact
    14581 gctatatggg tcgggctggg ttgataaact ccggcggtgc tgcgggcggt gaaactgacc
    14641 tcagcgatgc agtgcgtact gcggttatca acaaacgcgc tggcggaatg gggctgattc
    14701 ttggacgtaa ggcgttcaag aaatcgatgg ctgacggcgt gaaactgatt aatgccgtgc
    14761 aggacgttta tctcgatagc aaaattacta ttgcctgata cgcctcatac gaacagcacc
    14821 accccgtagg acggataagg cgtttacact gcatccggca acctatgcct gatgcgacgc
    14881 ggaagcgtct tatcatgcct acatagcact gccacgtatg tttacaccgc atccggcata
    14941 aaaacacgcg cactttgcta cg
//
LOCUS       DAZFQD010000129        14809 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.129, whole genome shotgun sequence.
ACCESSION   DAZFQD010000129 DAZFQD010000000
VERSION     DAZFQD010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14809
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.129"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..347
                     /locus_tag="ACHKTW_003992"
     CDS             <1..347
                     /locus_tag="ACHKTW_003992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
                     /protein_id="HFO6464918.1"
                     /translation="PDIVAELATIKDKSDDEWWRALLQSRNDHLRQTALRNAHTPASL
                     LTTLTESRHRSLAMNNPQLAADVKTTWLKEDPSLLLFVEQPDLSLLRDLVKTGAMRKI
                     RSEARHWLEEKQ"
     gene            344..427
                     /locus_tag="ACHKTW_003993"
     CDS             344..427
                     /locus_tag="ACHKTW_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6464919.1"
                     /translation="MTQRPWSKLQHKTHNIAALKIIARRSE"
     gene            complement(505..1416)
                     /gene="rdgC"
                     /locus_tag="ACHKTW_003994"
     CDS             complement(505..1416)
                     /gene="rdgC"
                     /locus_tag="ACHKTW_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HFO6464920.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWIDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            1541..2449
                     /gene="mak"
                     /locus_tag="ACHKTW_003995"
     CDS             1541..2449
                     /gene="mak"
                     /locus_tag="ACHKTW_003995"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HFO6464921.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNANSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRAHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVGQLIKQFVFGGE
                     CETPVRKAKHGDSSGVRGAAWLWPQE"
     gene            complement(2592..3776)
                     /gene="araJ"
                     /locus_tag="ACHKTW_003996"
     CDS             complement(2592..3776)
                     /gene="araJ"
                     /locus_tag="ACHKTW_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414930.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HFO6464922.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMGVLTELAHNVGISIPAAGHMI
                     SYYALGVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQEFSWRYTF
                     LLIAVFNIAVMASVYFWVPDIRDEAKGKLREQFHFLRSPAPWLIFAATMFGNAGVFAW
                     FSYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIV
                     LALLMLFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFN
                     LGSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSPVLAKP
                     LG"
     gene            complement(3902..7045)
                     /gene="sbcC"
                     /locus_tag="ACHKTW_003997"
     CDS             complement(3902..7045)
                     /gene="sbcC"
                     /locus_tag="ACHKTW_003997"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HFO6464923.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVALLTPEQV
                     QSLTASLQVLIDEEKQLLTAQQQEQQSLNWLTRLDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHTRQQIEEVNTRLQSTMALRASIRHHA
                     ANQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNTLAAITLTLTADEVASALAQHAEQRPLRQRLVALHGQIVPQQKRLAQLQVAIQ
                     NVTQEQTQRNAALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALAGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRIQQLTPILETLPQSDDLPHSEETVALDNWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLMQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHGRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            complement(7042..8244)
                     /gene="sbcD"
                     /locus_tag="ACHKTW_003998"
     CDS             complement(7042..8244)
                     /gene="sbcD"
                     /locus_tag="ACHKTW_003998"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HFO6464924.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIITSQAGLNGIEKQQHLLAAITDY
                     YQQQYADACKLRGDQPLPIIATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELDESQQQRLQHLFTTTLH
                     TLAGEHEA"
     gene            8434..9123
                     /gene="phoB"
                     /locus_tag="ACHKTW_003999"
     CDS             8434..9123
                     /gene="phoB"
                     /locus_tag="ACHKTW_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HFO6464925.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            9181..10476
                     /gene="phoR"
                     /locus_tag="ACHKTW_004000"
     CDS             9181..10476
                     /gene="phoR"
                     /locus_tag="ACHKTW_004000"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HFO6464926.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRLLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTHLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            10883..12202
                     /gene="brnQ"
                     /locus_tag="ACHKTW_004001"
     CDS             10883..12202
                     /gene="brnQ"
                     /locus_tag="ACHKTW_004001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HFO6464927.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVAAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFRLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            12278..13651
                     /gene="proY"
                     /locus_tag="ACHKTW_004002"
     CDS             12278..13651
                     /gene="proY"
                     /locus_tag="ACHKTW_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HFO6464928.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVWFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            13807..>14809
                     /gene="malZ"
                     /locus_tag="ACHKTW_004003"
     CDS             13807..>14809
                     /gene="malZ"
                     /locus_tag="ACHKTW_004003"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308480.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004558 - alpha-1,4-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HFO6464929.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVSMHKQRSQQQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGMRLVLDGVFNHSGDSHAWFDRHNRGTGGACHNPES
                     PWRDWYSFSDDGTALDWLGYASLPKLDYHSESLVNEIYRGEDSIVRHWLKAPWSMDGW
                     "
BASE COUNT         3249 a         3786 c         4009 g         3765 t
ORIGIN      
        1 ctccagatat tgttgcggaa ctggctacga taaaagataa atcagatgat gaatggtggc
       61 gtgcattgct gcaaagtcgc aatgatcatt tgcgccaaac ggcattacgc aacgcacata
      121 ccccggcgtc gttgttaacg acattgaccg agtctcggca tcggtcgcta gctatgaata
      181 atccacagct cgctgccgat gtgaaaacga cgtggttaaa agaggatcca tcattactct
      241 tatttgtcga acaacccgat ctttcgctgt tacgggattt agtgaaaacc ggggcaatgc
      301 ggaaaattcg cagtgaagcg cgtcactggc ttgaggaaaa acaatgacac aacgtccatg
      361 gagcaagtta cagcataaaa cgcataatat tgctgcgcta aaaattattg cccgccgtag
      421 cgaataatta tatgcctggt gtggcttcgt acgccggata agacgcggca ggcgtcgcat
      481 ccggcattaa aggaaaatca gcaattaacg ttgtgcttcg ccacctaatc cttcaatcag
      541 gttttgaatt aacgctgcca gttcaccagt catcaggatg aaatcggcat caaaacgctg
      601 ggcgaaatct tcacggtcga tatcttcgtt ttgatcgcgc agctcgtcgc agaacttcag
      661 acgcttaagc gaaccatcgt cgcacatcac aaactgaatg cgctgctgcc agtcgagcgc
      721 cagtttagtc accacttttc cggcttcaat gtgattggtg atctcttcgc tggtcagatc
      781 ttgtttcttc gcgcggatca cgccgccatc ttccagcaac gatttcagct cggcttcatc
      841 aagcagctgg aagccctgtg ccgcactacc ggagcgaacc cattcggtca gcgtcagttc
      901 aatcgggttt tccatgctca atggtacaac cggtaacgac cccaggcttt tacgcagtaa
      961 tgccagcgta tcttccgctt ttttggcact ggcgcagtcc accataatca aaccgttaac
     1021 cgtgtcgatc cacatcattg tctggctaaa acggctgaaa gcacgcggca gcagagagtg
     1081 cagcacttcg tctttcagcg aatctttttc ggttttcttc agcttacgcg cctgttccgc
     1141 ttccagtttg gcgattttcg cttcaagcgc ctgtttaatc accggagacg ggaggatttt
     1201 ttcttctttg cgcgcgcaga taacaatttg accattggca acgtgcgtta acgcatcgct
     1261 gtgcgatccc atcggaggaa cccagcccat cttcgccatg tcctggctgc cgcatggggt
     1321 aaatgccatc gaggctagct gtttttccat ctcttctgca cgcagcgaaa tctcgcggct
     1381 aagacggtaa accattaaat ttttgaacca cagcatgata atttccacgg ccttgtcgtt
     1441 aaatttagcg ggcatgataa cgaattgtcg gcggccttgc attgccaatc cggttgtccg
     1501 tctctacgct attgatattg aaaaaaataa ggagagtacc gtgcgtatag gtatcgattt
     1561 aggcggcacc aaaactgaag tgattgcact gggcgatgca ggggagcagt tgtaccgcca
     1621 tcgtctgccc acgccgcgtg atgattaccg gcaaactatt gaaacgatcg ccacgttggt
     1681 tgatatggcg gagcaggcga cagggcagcg cggaacggta ggtatgggca ttcctggctc
     1741 aatttcgcct tacaccggtg tggtgaagaa tgccaattca acctggctca acggtcagcc
     1801 attcgataaa gacttaagcg cgaggttgca gcgggaagtg cggctggcaa atgacgccaa
     1861 ctgtctggcg gtttcagaag cagtagatgg cgcggcagcg ggagcgcaga cagtatttgc
     1921 cgtgattatc ggcacgggat gcggcgcggg cgtggcattc aatgggcggg cgcatatcgg
     1981 cggcaatggc acggcaggtg agtggggaca caatccgcta ccgtggatgg acgaagacga
     2041 actgcgttat cgcgaggaag tcccttgtta ttgcggtaaa caaggttgta ttgaaacctt
     2101 tatttcgggc acgggattcg cgactgatta tcgtcgtttg agcggacatg cgctgaaagg
     2161 cagtgaaatt atccgcctgg ttgaagaaag cgatccggta gcggaactgg cattgcgtcg
     2221 ctacgagctg cggctggcaa aatcgctggc acatgtcgtg aatattctcg atccggatgt
     2281 gattgtcctg gggggcggga tgagcaatgt agaccgttta tatcaaacgg ttgggcagtt
     2341 gattaaacaa tttgtcttcg gcggcgaatg tgaaacgccg gtgcgtaagg cgaagcacgg
     2401 tgattccagc ggcgtacgcg gcgctgcgtg gttatggcca caagagtaaa aaacgtaggc
     2461 aattggcgca tcatgcttga tgcgacgctt gccgcgtctt atcaggccta caaaatgtgc
     2521 cggaaccgcg tagggcggat aaggcgttca cgccgcatcc ggcaataagc aacgcgcaac
     2581 gtgactgcaa tctaccccag tggtttcgcc agcaccggac tatccgccgc ttgctggcgc
     2641 ttatagcgac catacagcag caatgacgac atcgcagcaa acgaaagcag ggcggcaggc
     2701 agcgccacgt aattatatgc cagccccagc gtcagcatca taccaccgca atatgcgccg
     2761 acggcgctac caaggttaaa cgctatttgc ccacctgcgg cacctaataa ctctccgcct
     2821 ttggcatttt gtagtaataa tatttgcaga ggtgctgaaa gggcaaataa tcccgcgcaa
     2881 caaataaaag caaaaataag cgacgttgtt ttcatgccgc cgcagaaaaa gagcattagc
     2941 agtgccagta caattataaa gtcagtcact gctgcaatgc gcagtggtga ataacgtcct
     3001 gaaatcctgc cacttagcat atttcccaac accatcccta gcccaactaa catcataata
     3061 aaggtcatcg ccgtttccga aaaaccggaa ataaacatca tgtatggctt tacgtagctg
     3121 aaccaggcaa acacacctgc gttgccaaac atcgtggcgg cgaaaattaa ccacggggcc
     3181 gggctgcgta aaaagtgaaa ttgttcgcgc agctttcctt tcgcctcgtc gcgaatatcc
     3241 ggcacccaaa aatagaccga tgccatcacc gcaatattaa aaacagcgat caataaaaag
     3301 gtgtaacgcc agctaaattc ctgacttaaa tacgttccca gcggaatgcc cagcaaattg
     3361 gcgactgtca tcccggaaac catccccgcc acggcggcgg tgacttttcc gggtttgata
     3421 atttttgata acacgatcgc gccgacgcca aaaaatgcgc catgcggaaa gccggaaacc
     3481 aggcgaccaa tggcaagcat caggtaagac gaagagagcg tgaacatggc gttgccaatg
     3541 acgcacaacg ccaccagaaa caacaagata tgtttgagtg agtagcggct ggaaaagagt
     3601 gcgatgattg gcgcaccgac caccactccc agcgcataat acgagatcat atgcccggca
     3661 gcaggaatcg aaattcctac gttatgcgcc agctccgtga gcacgcccat aatgccaaat
     3721 tcggccatcc ccaaaccaaa cgtgcccaga gccaaagata aaatgacttt tttcatacca
     3781 ccatcacaac taccagaacg gctaccaatc accgtagcca ataaagtgat agcttgcagg
     3841 ataacgacca gcaaggccaa gagataattc gcccctctgt attcattatc ctgctgaata
     3901 gttatttcac tgcaaacgta ctttccagtt tgctgtagcc caggccgttg atctttttca
     3961 ctttgatctg caccggaata cgctctttca tcgcttctac gtggctaatc acaccgatgg
     4021 ttttgccact ggcgttcagg gcatccagcg catcaagggc ggtatccagc gtttcgctat
     4081 ccagcgtgcc aaaaccttca tcaaggaaca gcgagtcaat acgtgtttta tggctgacca
     4141 gatccgaaag cgccagcgcc agcgccagac taacgaggaa actttcgccg ccggaaaggg
     4201 tacgggtatc gcgtaccgca tctgcctgcc aggtatcaac aacctcgact tccagcgcct
     4261 cgctggcttt gcgctgtaac agatagcgcc cgtgcagccg ggtaagttgc tgattagcga
     4321 gatggactaa attatccagc gtcagcccct gggcaaactt gcggaattta tcgccctctt
     4381 tggaacctat tagcgaattc agatatcccc agtcctcaac ctgctgcgtc atttgagcaa
     4441 tttgctgcat taaggtttgt tgttgctgac ggttatctgc atcctgcttc agctgctggc
     4501 gaatctcgcc ttgactcgtg gtgttttcac gcaacttttg gtgagtttgc gctaactctt
     4561 gctgaatctg ctccaccgtc acagtgagag ccaacccgtc aggtcggtgt tgttgatgct
     4621 gtgccagcgt ttctgctgtc tgagtgacca gcgtttgcgc ctgacggcgc tggttttcca
     4681 gattctgctt gagctgttcc agctgcgtta gtgtttgttc atccattagc gccgcaagga
     4741 acgcctgctg atcgtcaaag acgctggcct gtagcgcggt gtcaaactgc gcctgggctt
     4801 tttgcagact ttgcgccgcc agaacatcct gttgctgtaa cgtctgctgc tggctgtgta
     4861 atgcgagaca ttgttcatga acctgccgcc agttatccag cgccacagtt tcttcgctgt
     4921 gcgggagatc atcactttgc ggcaacgttt ccagaatcgg cgtcagctgc tgaatacggt
     4981 tttgcagcgc ggttaattcg ttctggcgtt gctgccagct ctgcgcttct tgctgacgtg
     5041 tcgccaacca gctctcttct tcatcttcct gtggcaatgt cagtgcataa cccgccaatg
     5101 ccgttaagag ctgctgctgg cgttgttcaa tttgctgttg atactggata atttgctgat
     5161 tatgcgcggc aatctgccct tgtaattcat gtcgttggct gagtaaccgc agctggcgtt
     5221 cgtgctcatc ttgtgcatca agccacggtt gaatatcgtc ctgtggctgc aaggtgatat
     5281 tgaggctggc cgtgacggct tgccattgtt gagtaagtgc ttgctcatct tgtcggaggc
     5341 tttgcgcttc gttttcatcg cgctgaagct gcttcgttaa tgcatccagt tgcccgcgca
     5401 gcgccgcacc ttcttcgccg agctttttaa cttcgttttc cagcgccagt aatcgagact
     5461 gattaacgcc aggctccagc gcctgatacg cctcgaccgc cgggtggctg gtggaaccac
     5521 aaagtggaca aggctgaccc gcctgtaact gtgcacgttg agcttccagc gttttgatgc
     5581 gcgcttcctg ctcgcaaatg gttttcacat cggcaagttg ctgcgtcttt tctttataac
     5641 gctggcgcat ttcgttaagt gcggcgttac gttgcgtctg ttcttgcgtg acattctgga
     5701 tagcgacctg taactgcgcc agacgttttt gttggggaac aatctgccca tgcagcgcga
     5761 ccaggcgctg acgcagtggg cgttgctcgg catgttgcgc cagggcgcta gcaacttcat
     5821 cggcggttaa cgtcaacgtg atcgccgcaa gcgtattaag tttttgctca gcatgggtta
     5881 actgttgctg ccattgccgc agatgctcgc gatcgctggt ttgttgggag aactgcgcac
     5941 gccaacccgc cagttcgttg ttccactgac ggaagcggtc gtgttcctgt aaccaggtat
     6001 tcaggctttg ttgctgctgc tgtaattctg ctgactgatt cgccgcgtgg tggcgaatgc
     6061 tcgcgcgaag cgccattgtg ctctgtaagc gagtatttac ttcttcaatc tgctggcgag
     6121 tatgcgccag cgccgcgctg tgttctgcga tgcgttccca gtgcggacga agatttcgtg
     6181 ccggttgtgc cagactgagc gccgccagtt gaggttgcgc tttttcttct tcggctaacg
     6241 cctgttgcaa ggcctgctga cggcggctgg cttcttgctg caattcgtcc agacgcgtta
     6301 accagtttag tgattgttgt tcttgctgct gcgcggtaag taactgtttt tcttcgtcaa
     6361 taagtacctg caaactcgct gtcagcgatt gcacttgttc cggcgtgagc aacgcgacgc
     6421 cgctggcctg cgcttgcagc ttctccagct ctgtgcgggc cgatttgtgc tgctcaaaaa
     6481 ccatcgccga gatttgcccg tagatttcag tgccggttaa ctcctcgagc aattccgcgc
     6541 gttctttggg tttggcattc aggaaggcag caaattgccc ctgcgaaagc agcatcgaac
     6601 gggtgaagcg cccgtaatcc agcccggtta acgtcgctgt cagttccagc ttatctttca
     6661 ctttgtcggc gagaattttg ccgtcggcgc agcgcgccag ctctacgcgt ggcacctgca
     6721 aattaccgtc gggttggtta cgcgcccgat tctggctcca gaatgcacgg tacgcttcac
     6781 ctttcacttc aaactccacc tccgccagac attcggcggt atcgcgggtc atgagatcat
     6841 tttgcgattg tgaaacgtta gagagacgcg gagtttcgtg atacagcgcc agacaaatgg
     6901 cgtccagcag ggtggttttc cccgcacctg ttgggccggt aatagcaaac agcccgttgc
     6961 tggcgaacgg ctcgcgggtg aaatcaatct tccattcgcc ttttaatgag ttcaggtttt
     7021 tcaggcgcag gctgagaatt ttcatgcttc gtgttctccg gcgagggtat gcaacgtcgt
     7081 ggtgaaaaga tgctgcagac gttgctgctg cgattcatcc agttcttcca gtgccagacg
     7141 gcgattgaac acctcttcga cgctgagttc gctgagggtt tcacgctgtt ggctggctaa
     7201 cacacgctcg cgctgttcac gactccggcg taccagcaat acttcaacag gcaatgattc
     7261 ggttaatgcc tggattttgc gctgaatatc atgcagatac tcatcagtag tgatttcgat
     7321 atccagccag acaggcggct cctgtgatac atcgcgccac tgttccagct gtgcggtaat
     7381 cgacgccaga tcgcctttca gcaccgccat gggttgcgtt accggtacgt tcaggttttc
     7441 cacgctctct aatttgccgt ttgaaaatgt caccagatgg acatatttac tctttccgca
     7501 ttcatcaaaa ctcagtggaa tgggagagcc gcaatagcga acatgttcca tgccgccaat
     7561 aatctgtgcg cggtgaatat gcccgagcgc gatgtagtcg gctggtggaa agttttgtgc
     7621 tggaaacgcg tccagcgtgc caatataaat gtcacgcacg gcgtcacttt tactggcacc
     7681 cacggtcgtt aagtgtcccg tggcgatgat gggcagaggc tgatcgccgc gcagtttgca
     7741 ggcatcggca tattgttgtt ggtaataatc ggtaatcgct gccagtaaat gctgctgttt
     7801 ttcaataccg ttaagccccg cctggctggt aataatgtca cgcggacgta aaaacggaat
     7861 ggggcacagc actgcgcctg gcgtcccgtc gcgacgaggc aagatttgcg gcgcatgtcc
     7921 ggcgctggcg accacggttg tattgaggaa cgccatgata tcgcgcgatt cattcagcgt
     7981 ggcgaccgag tcatggtttc ctgccagtac caccagatga cagccagttt gctgtaaatt
     8041 gacaacaaaa cggttgtata acgtgcgggc gtaactgggc ggcgagccgg tatcgaaaac
     8101 atcaccagca acaataatcg catccacctg atgggtttgt gctgtttcca gcagccagtc
     8161 aagaaaagcc tgatgttcag cttcgcggct tttactgtag aagttctggc cgagatgcca
     8221 gtctgaggtg tgaaggatgc gcataacgat tccctggcga aaaagcatgg gcgcgattat
     8281 acccaaacag atgtgccatt tgcttttttc tgcgccacgg aaatcaataa cctgaagata
     8341 tgtgcgacga gcttttcata aatctgtcat aaatctgacg cataatgacg tcgcattaat
     8401 gatcgcaacc tatttattac aacagggcaa atcatggcga gacgtattct ggtcgtagaa
     8461 gatgaagctc caattcgcga aatggtctgc ttcgtgctcg aacaaaatgg ctttcagccg
     8521 gtcgaagcgg aagattatga cagtgctgtg aatcaactga atgaaccctg gccggattta
     8581 attctccttg actggatgtt acctggcggc tccggtatcc agttcatcaa acacctcaag
     8641 cgcgagtcga tgacccggga tattccagtg gtgatgttga ccgccagagg ggaagaagaa
     8701 gatcgcgtgc gcggccttga aaccggcgcg gatgactata tcaccaagcc gttttcgccg
     8761 aaggagctgg tggcgcgaat caaagcggta atgcgccgta tttcgccaat ggcggtggaa
     8821 gaggtgattg agatgcaggg attaagtctc gacccgacat ctcaccgagt gatggcgggc
     8881 gaagagccgc tggagatggg gccgacagaa tttaaactgc tgcacttttt tatgacgcat
     8941 cctgagcgcg tgtacagccg cgagcagctg ttaaaccacg tctggggaac taacgtgtat
     9001 gtggaagacc gcacggtcga tgtccacatt cgtcgcctgc gtaaagcact ggagcccggc
     9061 gggcatgacc gcatggtgca gaccgtgcgc ggtacaggat atcgtttttc aacccgcttt
     9121 taacgccttg ctcatcggac gcagagcagg gcttatgatt tcttaactgg agtatcttac
     9181 gtgctggaac ggctgtcgtg gaaaaggctg gtgctggagc tgctactttg ctgcctcccg
     9241 gctttcatcc tgggtgcatt ttttggttac ctgccctggt ttttgctggc atcggtaaca
     9301 ggactgctta tctggcattt ctggaattta ttgcgccttt catggtggct gtgggtggat
     9361 cgcagtatga ccccgccacc ggggcgtggt agctgggaac cgctactata cggcttacac
     9421 cagatgcagc tgcgaaataa aaaacgccgc cgtgaactgg gcaatctgat taaacgcttt
     9481 cgtagcggcg cggagtcgct gcccgacgcg gtggtgctga ccacggaaga gggcggtatt
     9541 ttctggtgta acggtctggc gcaacaaatt cttggtttgc gctggccgga agataacggg
     9601 cagaacatcc ttaacctact gcgttacccg gagtttacgc aatatctgaa aacgcgtgat
     9661 ttttctcgcc tgctcaatct ggtgctcaac accgggcggc atctggaaat tcgcgtcatg
     9721 ccttataccc acaaacagtt gctgatggtg gcgcgtgatg tcacgcaaat gcatcaactg
     9781 gaaggggcgc ggcgtaactt ttttgccaac gtgagccatg agttacgcac gccattgacc
     9841 gtgttacagg gttacctgga gatgatggat gagcagccgc tggaaggcgc ggtacgcgaa
     9901 aaagcgttgc acaccatgcg cgagcagacc cagcggatgg aagggctggt gaagcagttg
     9961 ctgacgctgt cgaaaataga agccgcaccg acgcatttgc tcaatgaaaa ggttgatgtg
    10021 ccgatgatgc tgcgcgttgt tgagcgtgag gctcagactc tgagtcagaa aaaacagaca
    10081 tttacctttg agatagataa cggcctcaaa gtgtctggca acgaagatca gctacgcagt
    10141 gcgatttcga acctggtcta taacgccgtg aatcatactc cagaaggcac gcatatcacc
    10201 gtacgctggc agcgagtgcc gcacggtgcc gaatttagcg ttgaagataa cggaccgggc
    10261 attgcaccgg agcatattcc gcgcctgacc gagcgttttt atcgcgttga taaagcgcgt
    10321 tcccggcaaa ccggcggtag cggattaggg ttagcgatcg tgaaacatgc ggtgaatcat
    10381 cacgaaagtc gcctgaatat tgagagtaca gtaggaaaag gaacacgttt cagttttgtt
    10441 atcccggaac gtttaattgc caaaaacagc gattaatccg cctttgtcat cttttattgc
    10501 cataagccag ccgatgctgg cttattttct ttgcagtcaa aatacgggcg ttagatttta
    10561 caacggttgg tgattttttg ttcgcatgat tagccatgtc tttttcacgg aaatagtgtt
    10621 ttatactggt tggtgatttc ttatcgctat atacctctgg tttttagatc cctccttgct
    10681 ttaaaacgtt ataagcgttt aaattgcgct tcaggtgctg tcatactgac tgcattaacg
    10741 cggtaaatcg aaaaactatt cttcgccgcg cctggttggg agtatttccc gctaaaattg
    10801 tttaaatata ccgctgtatc atccccaggg attggcacaa aaatttaacg ttacaacacc
    10861 acatccacag gcagtatgat ttatgaccca tcaattaaga tcgcgcgata tcatcgctct
    10921 gggctttatg acatttgcgt tgttcgtcgg cgcaggtaac attattttcc ctccaatggt
    10981 cggcttgcag gcaggcgaac acgtctggac tgcggcattc ggcttcctca ttactgccgt
    11041 tggtctgccg gtactaacgg tagtggcgct ggcaaaagtt ggcggcggtg ttgacagcct
    11101 cagcacgcca atcggtaaag tcgctggcgt actgctggca acggtttgtt acctggcggt
    11161 ggggccgctt ttcgctacgc cgcgtacagc taccgtttcc tttgaagtgg ggattgcgcc
    11221 gctgactggt gattccgcgc tgccgctgtt tatctacagc ctggtctatt tcgctatcgt
    11281 tattctggtt tcgctctatc cgggcaagct gctggatacc gtgggcaact tccttgcgcc
    11341 gctgaaaatt atcgcgctgg tcatcctgtc tgttgccgcg attgtctggc cggcgggttc
    11401 tatcagcacg gcgactgagg cttatcaaaa cgctgcgttt tctaacggct tcgttaacgg
    11461 ctatctgacc atggatacgc tgggcgcaat ggtgtttggt atcgttattg ttaacgcggc
    11521 gcgttctcgt ggcgttaccg aagcgcgtct gctgacccgt tataccgtct gggctggcct
    11581 gatggcgggt gttggtctga ctctgctgta cctggcgctg ttccgtctgg gttcagacag
    11641 cgcgtcgctg gtcgatcagt ctgcaaacgg cgctgctatt ctgcatgctt acgttcagca
    11701 cacctttggc ggcggcggta gcttcctgct ggcggcgtta atcttcatcg cctgcctggt
    11761 aacggcagtt ggcctgacct gtgcttgtgc agaattcttt gcccagtacg taccgctctc
    11821 ttatcgtacg ctggtgttta tcctcggcgg cttctcgatg gtggtttcta acctcggctt
    11881 aagccagctg atccagatct ccgtaccggt gctgaccgct atttatccgc cgtgtatcgc
    11941 actggttgta ttaagtttta cacgctcatg gtggcataat tcgtcccgcg tgattgctcc
    12001 gccgatgttt atcagcctgc tttttggtat tctcgacggg atcaaagcat ctgcattcag
    12061 cgatatctta ccgtcctggg cgcagcgttt accgctggcc gaacaaggtc tggcgtggtt
    12121 aatgccaaca gtggtgatgg tggttctggc cattatctgg gatcgcgcgg caggtcgtca
    12181 ggtgacctcc agcgctcact aaatcactga acatttgttt taaccacggg gctgcgatgc
    12241 cccgtggttt tttattgtgt tgatgggtta ggaattgatg gaaagtaaga acaagctaaa
    12301 gcgtgggcta agtacccgcc acatacgctt tatggcactg ggttcagcaa ttggcaccgg
    12361 gctgttttac ggttcggcag acgccatcaa aatggccggt ccgagcgtgt tgttggccta
    12421 tattatcggt ggtatcgcgg cgtatatcat tatgcgtgcg ctgggggaaa tgtcggtaca
    12481 taacccggca gccagctctt tctcgcgtta tgcgcaggaa aacctcggcc cgctggcagg
    12541 ttacattacc ggctggacct attgctttga aatccttatt gtcgccatcg ccgatgtgac
    12601 cgcttttggt atctatatgg gtgtctggtt cccgacggtg ccgcactgga tttgggtact
    12661 gagcgtggtg ctgatcattt gcgccgtaaa cctgatgagc gtgaaggtct tcggtgagct
    12721 ggaattctgg ttctcgttct ttaaagtcgc caccatcatc atcatgattg tcgccggttt
    12781 cggcatcatc atctggggga ttggcaacgg cgggcaaccg accggtattc ataacctgtg
    12841 gagcaacggc ggcttcttca gtaacggctg gcttgggatg gtgatgtcgt tgcaaatggt
    12901 gatgtttgct tacggtggga tcgaaattat cgggattacc gccggtgaag cgaaagatcc
    12961 tgagaaatcg ataccgcgtg cgattaactc cgtgccgatg cgtattctgg tgttctacgt
    13021 cggtacgctg ttcgtcatta tgtctatcta cccgtggaat caggttggca ctgccggtag
    13081 cccgttcgtg ctgacgttcc agcatatggg cattaccttt gccgccagca ttcttaactt
    13141 tgttgtgctg actgcttcgc tgtcggcaat taacagtgac gtatttggcg taggccgtat
    13201 gctccacggt atggcagagc agggcagcgc gccgaaaatt ttcagcaaaa cgtcgcgtcg
    13261 cggtattccg tgggttacgg tgctggtgat gactaccgcg ctgctgtttg cggtgtatct
    13321 gaactacatc atgccggaaa acgtcttcct ggtgatcgct tcgctggcaa ccttcgccac
    13381 ggtgtgggtg tggattatga tcctgctgtc gcaaattgcc ttccgtcgcc gtttgccgcc
    13441 agaagaagtt aaggcgctga aatttaaagt gccgggtggg gtagcaacga ccatcggcgg
    13501 tttgattttc ctgctcttta ttatcgggtt gattggttat cacccggata cgcgtatctc
    13561 gctgtatgtc ggtttcgcgt ggattgttgt gctgttgatt ggctggatgt ttaaacgccg
    13621 ccacgatcgt cagctggctg aaaaccagta atccttgcgt tctgtaagcc ggttcaggag
    13681 gaaatcctga tccggctatt ccgaaagtta tccgcccccg tcctcctccc ccaaatatcc
    13741 ttcagatgat gagtgatcct gcattaggct atggcaaggt gatcagattt tcatcacagg
    13801 ggaattatga tgttaaatgc atggcacctg ccggtgcccc catttgttaa acaaagcaaa
    13861 gatcaactgc tcattacact gtggctgacg ggcgaagacc caccgcagcg cattatgctg
    13921 cgtacagaac acgataacga agaaatgtca gtatcgatgc ataagcagcg cagtcagcag
    13981 cagcctggcg tcaccgcatg gcgtgcggcg attgatctct ccagcggaca accccggcgg
    14041 cgttacagtt tcaaactgct gtggcacgat cgccagcgtt ggtttacacc gcagggcttc
    14101 agccgaatgc cgccggcacg actggagcag tttgccgtcg atgtaccgga tatcggccca
    14161 caatgggctg cggatcagat tttttatcag atcttccctg atcgttttgc gcgtagtctt
    14221 cctcgtgaag ctgaacagga tcatgtctat taccatcatg cagccggaca agagatcatc
    14281 ttgcgtgact gggatgaacc ggtcacggcg caggcgggcg gatcaacgtt ctatggcggc
    14341 gatctggacg ggataagcga aaaactgccg tatctgaaaa agcttggcgt gacggcgctg
    14401 tatctcaatc cggtgtttaa agctcccagc gtacataaat acgataccga ggattatcgc
    14461 catgtcgatc cgcagtttgg cggtgatggg gcgttgctgc gcttacgcca caatacgcag
    14521 cagctgggaa tgcggctggt gctggatggc gtgtttaacc acagtggcga ttcccatgcc
    14581 tggtttgaca ggcacaatcg tggcacgggg ggagcttgtc acaaccctga atcgccctgg
    14641 cgcgactggt actcgtttag tgatgatggc acggcgctcg actggcttgg ctatgccagc
    14701 ttgccgaagc tggattatca ctcggaaagt ctggtgaatg aaatttatcg cggggaagac
    14761 agtattgtcc gccattggct gaaagcgccg tggagtatgg acggctggc
//
LOCUS       DAZFQD010000130        14809 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.130, whole genome shotgun sequence.
ACCESSION   DAZFQD010000130 DAZFQD010000000
VERSION     DAZFQD010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14809
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.130"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..129
                     /locus_tag="ACHKTW_004004"
                     /pseudo
     CDS             1..129
                     /locus_tag="ACHKTW_004004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278583.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(151..339)
                     /locus_tag="ACHKTW_004005"
     CDS             complement(151..339)
                     /locus_tag="ACHKTW_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1869 domain-containing protein"
                     /protein_id="HFO6464930.1"
                     /translation="MGKATYTVTVTNNSNGVSVDYETETPMTLLVPEVAAEVIKDLVN
                     TVRSYPVRRKTPSFRAGI"
     gene            complement(343..702)
                     /gene="yeaR"
                     /locus_tag="ACHKTW_004006"
     CDS             complement(343..702)
                     /gene="yeaR"
                     /locus_tag="ACHKTW_004006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1971 domain-containing protein YeaR"
                     /protein_id="HFO6464931.1"
                     /translation="MLQIPQNYIHTRSTPFWNKQTAPAGIFERHLDKGTRPGVYPRLS
                     VMHGAVKYLGYADEHSAEPDQVILIEAGQFAVFPPEKWHNIEAMTDDTYFNIDFFVAP
                     EVLMEGAQQRKVIHNGK"
     gene            complement(875..1513)
                     /gene="leuE"
                     /locus_tag="ACHKTW_004007"
     CDS             complement(875..1513)
                     /gene="leuE"
                     /locus_tag="ACHKTW_004007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine efflux protein LeuE"
                     /protein_id="HFO6464932.1"
                     /translation="MFAEYGVLNYWTYLVGAIFIVLVPGPNTLFVLKNSVSSGMKGGY
                     LAACGVFIGDAVLMFLAWAGVATLIKTTPILFNIVRYLGAFYLLYLGSKILYATLKGK
                     NNEAKSDEPQYGAIFKRALILSLTNPKAILFYVSFFVQFIDVNAPHTGISFFILATTL
                     ELVSFCYLSFLIISGAFVTQYIRTKKKLAKVGNSLIGLMFVGFAARLATLQS"
     gene            complement(1640..2563)
                     /gene="dmlR"
                     /locus_tag="ACHKTW_004008"
     CDS             complement(1640..2563)
                     /gene="dmlR"
                     /locus_tag="ACHKTW_004008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DmlR"
                     /protein_id="HFO6464933.1"
                     /translation="MNNLPLLNDLRVFMLVARRAGFAAVAEELGVSPAFVSKRIALLE
                     QTLNVVLLHRTTRRVTITEEGERIYEWAQRILQDVGQMMDELSDVRQVPQGMLRIISS
                     FGFGRQVVAPALSALAKAYPQLELRFDVEDRLVDLVNEGVDLDIRIGDDIAPNLIARK
                     LATNYRILCASPEFIAQHGAPKHLTDLSALPCLVIKERDHPFGVWQLRNKEGHHAIKV
                     TGPLSSNHGEIVHQWCLDGQGIALRSWWDVSENIASGHLVQVLPEYYQPANVWAVYVS
                     RLATSAKVRITVEFLRQYFAEHYPNFSLEHA"
     gene            2666..3751
                     /gene="dmlA"
                     /locus_tag="ACHKTW_004009"
     CDS             2666..3751
                     /gene="dmlA"
                     /locus_tag="ACHKTW_004009"
                     /EC_number="1.1.1.83"
                     /EC_number="1.1.1.85"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional
                     D-malate/3-isopropylmalate/tartarate dehydrogenase"
                     /protein_id="HFO6464934.1"
                     /translation="MMKTMPIAAIPGDGIGKEVLPEGIRVLQAAAERWGFALSFEQME
                     WASCEYYSHHGKMMPDDWHEQLSRFDAIYFGAVGWPDTVPDHISLWGSLLKFRREFDQ
                     YVNLRPVRLFPGVPCPLAGKQPGDIDFYVVRENTEGEYSSLGGRVNEGTEHEVVIQES
                     VFTRRGVDRILRYAFELAQSRPRKTLTSATKSNGLAISMPYWDERVEAMAENYPEIRW
                     DKQHIDILCARFVMQPERFDVVVASNLFGDILSDLGPACTGTIGIAPSANLNPERTFP
                     SLFEPVHGSAPDIYGKNIANPIATIWAGAMMLDFLGNGDERFQQAHNGILAAIEEVIA
                     HGPKTPDMKGNATTPQVADAICKIILR"
     gene            4002..5612
                     /gene="yeaV"
                     /locus_tag="ACHKTW_004010"
     CDS             4002..5612
                     /gene="yeaV"
                     /locus_tag="ACHKTW_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter YeaV"
                     /protein_id="HFO6464935.1"
                     /translation="MMSNVKKKDVPLISISLVAILFIAAALSLFPQQSADAANAIYTF
                     VTRTLGSAVQVLVLLAMGLVIYLATSKYGNIRLGEGKPEYSTLSWLFMFICAGLGSST
                     LYWGVAEWAYYYQTPGLNIAPRSQQALEFSVPYSFFHWGISAWATYTLASLIMAYHFH
                     VRKNKGLSLSGIIAAITGVRPQGPWGKLVDLMFLIATVGALTISLVVTAATFTRGLSA
                     LTGLPDNFTVQAFVILLSGGIFCLSSWIGINNGLQRLSKMVGWGAFLLPLLVLIVGPT
                     EFITNSIINAIGLTTQNFLQMSLFTDPLGDGSFTRNWTVFYWLWWISYTPGVAMFVTR
                     VSRGRKIKEVIWGLILGSTVGCWFFFGVMESYAIHQFINGVINVPQVLETLGGETAVQ
                     QVLMSLPAGKLFLAAYLGVMIIFLASHMDAVAYTMAATSTRNLQEGDDPDRGLRLFWC
                     VVITLIPLSILFTGASLETMKTTVVLTALPFLVILLVKVGGFIRWLKQDYADIPAHQV
                     EHYLPQTPVEALEKTPVLPAGTVFKGDN"
     gene            5644..6768
                     /gene="yeaW"
                     /locus_tag="ACHKTW_004011"
     CDS             5644..6768
                     /gene="yeaW"
                     /locus_tag="ACHKTW_004011"
                     /EC_number="1.14.13.239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase, oxygenase subunit YeaW"
                     /protein_id="HFO6464936.1"
                     /translation="MSNLSPDFVLPENFCANPQEAWTIPARFYTDQNAFEHEKENVFA
                     KSWICVAHSSELANANDYVTREIIGESIVLVRGRDKVLRAFYNVCPHRGHQLLSGEGK
                     AKNVITCPYHAWAFKLDGNLAHARNCENVANFDSDKAQLVPVRLEEYAGFVFINMDPN
                     ATSVEDQLPGLGAKVLEACPEVHDLKLAARFTTRTPANWKNIVDNYLECYHCGPAHPG
                     FSDSVQVDRYWHTMHGNWTLQYGFAKPSEQSFKFEEGTDAAFHGFWLWPCTMLNVTPI
                     KGMMTVIYEFPVDSETTLQNYDIYFTNEELTDEQKSLIEWYRDVFRPEDLRLVESVQK
                     GLKSRGYRGQGRIMADSSGSGISEHGIAHFHNLLAQVFKD"
     gene            6824..7789
                     /gene="yeaX"
                     /locus_tag="ACHKTW_004012"
     CDS             6824..7789
                     /gene="yeaX"
                     /locus_tag="ACHKTW_004012"
                     /EC_number="1.14.13.239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase reductase subunit YeaX"
                     /protein_id="HFO6464937.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHII
                     VQMSDGDNQYSNAYSLLSSPHDTSCYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIAGGIGITPFLSHMAELQHSDVDWQLHYCSRNPESCAFRDELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALIEAVRSEAARLDIAAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(7843..8958)
                     /gene="rnd"
                     /locus_tag="ACHKTW_004013"
     CDS             complement(7843..8958)
                     /gene="rnd"
                     /locus_tag="ACHKTW_004013"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0033890 - ribonuclease D activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HFO6464938.1"
                     /translation="MITTDDALASLCEAVRAFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEEALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(9040..10725)
                     /gene="fadD"
                     /locus_tag="ACHKTW_004014"
     CDS             complement(9040..10725)
                     /gene="fadD"
                     /locus_tag="ACHKTW_004014"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HFO6464939.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGMIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            complement(10930..11511)
                     /gene="yeaY"
                     /locus_tag="ACHKTW_004015"
     CDS             complement(10930..11511)
                     /gene="yeaY"
                     /locus_tag="ACHKTW_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HFO6464940.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSPTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADMN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQVTGYKRWHLTQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVQTVVTE"
     gene            complement(11551..12246)
                     /gene="tsaB"
                     /locus_tag="ACHKTW_004016"
     CDS             complement(11551..12246)
                     /gene="tsaB"
                     /locus_tag="ACHKTW_004016"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFO6464941.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLSAIDARMGEVYWAEYQRDENGIWHGEETEAVLKPEIVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            complement(12304..14214)
                     /gene="yoaA"
                     /locus_tag="ACHKTW_004017"
     CDS             complement(12304..14214)
                     /gene="yoaA"
                     /locus_tag="ACHKTW_004017"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HFO6464942.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDNRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            14346..14690
                     /gene="yoaB"
                     /locus_tag="ACHKTW_004018"
     CDS             14346..14690
                     /gene="yoaB"
                     /locus_tag="ACHKTW_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFO6464943.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
BASE COUNT         3584 a         3928 c         3666 g         3631 t
ORIGIN      
        1 gatcgaccag atattgcagt caggtattac tacaaaggcg ttttgtggag tcctggctat
       61 tttgccagta gctgcggagg tgcgccaata tccgtcatcc gccaatacat tgaacaacag
      121 caaacacctg gtcaggtgga aaaccgcgcc ttatatcccc gccctgaagg acggggtttt
      181 acggcgcacc ggataagaac gcacggtatt caccagatct tttatcactt cagccgccac
      241 ttctggcacc agcaaagtca tcggcgtctc tgtttcataa tcgacagaaa cgccattgct
      301 gttattggtg acggtcacgg tatacgttgc tttgcccatg attcatttcc cgttatgaat
      361 gactttccgt tgttgcgcac cttccatcag gacttcagga gccacgaaga agtcaatgtt
      421 gaaataagta tcgtcagtca tggcttcaat gttgtgccac ttttctggag ggaacaccgc
      481 aaactgcccc gcttcgataa ggatcacctg atcaggctct gcactgtgtt catcagcgta
      541 gccgagatat ttgaccgccc catgcataac ggaaaggcgt gggtaaaccc ccgggcgcgt
      601 tcctttatca agatgacgtt cgaatattcc ggcaggcgca gtttgtttat tccagaaagg
      661 cgttgagcgc gtatgaatat aattctgtgg gatttgcagc atcctattcc ctccttcggg
      721 gaatgcgctg aaaacggttt attccagccg tttcagggta cgcctgataa tttgcatttt
      781 aaataccatt tattggttac tttttagcac catatcagag aaaaatcagg gaggattaca
      841 agtgggaagc ccgcgacaac gcgggctgaa tgtatcagga ttgcagcgtc gccagtcggg
      901 cggcgaaacc cacgaacatc aaaccaatca gtgagttgcc aactttagcc agtttctttt
      961 tggtacgtat gtactgcgtg acaaaagcac cagaaataat caggaagctc aaatagcaga
     1021 aactcaccag ttccagcgtc gtcgccagaa taaagaatga aattcccgta tgtggggcat
     1081 taacatcgat aaactgtacg aaaaacgaca catagaacaa aatggctttc ggattagtca
     1141 ggctcaaaat taacgcgcgt ttaaaaatgg ccccgtattg gggctcatcg gatttggcct
     1201 cgttattttt acccttcagg gtcgcgtaga gaattttact ccccagatag agcaaataaa
     1261 acgcaccaag ataacgtaca atgttgaata atatcggggt ggtcttaatt aatgtcgcca
     1321 ctccagccca tgccagaaac atcaatactg catcgccaat aaatacaccg caggccgcaa
     1381 gataaccgcc tttcataccg ctactgacgc tatttttgag tacaaacagg gtatttggcc
     1441 ctggcaccaa cacaataaaa atggccccaa ccagataggt ccagtaattc agaaccccgt
     1501 attcagcgaa cacattaacc tctttaatta tctttcgatc atgcgcgatt aaaggtgaat
     1561 atgctaacca atctgtagcg gcttagaaag gagaaaatca ggttttaacc cgatatcaac
     1621 ccgataattg aatcattaat caggcatgct ccagtgaaaa attcgggtag tgctcggcaa
     1681 aatactggcg taaaaattct accgttatcc gcactttcgc tgacgtcgcc agccttgaaa
     1741 cataaacggc ccagacgttc gctggctggt aatattccgg tagcacttgc actaaatgac
     1801 cactggcaat gttttcgcta acatcccacc aggagcgcag agcaatcccc tgcccatcga
     1861 gacaccactg atggacaatt tctccatgat tagacgacag tgggccggtg actttaatcg
     1921 catggtggcc ttctttattg cgtaattgcc agacaccaaa aggatgatcg cgctctttaa
     1981 tgaccaggca cggcaatgcg gaaagatcgg ttaaatgttt cggcgcgcca tgttgcgcaa
     2041 taaattccgg cgacgcgcag agaatgcggt aattagtcgc cagtttgcgg gcgattaaat
     2101 taggggcgat gtcatcgcca atgcgaatat cgagatcaac accttcattg accaaatcga
     2161 ccaaccggtc ttccacatca aaacgtaatt ccagttgcgg atacgcctta gccagcgctg
     2221 ataacgccgg agccaccacc tgtcgtccaa aaccaaagct gctgataata cgcagcatcc
     2281 cctgcggcac ctgacgcacg tcagaaagtt cgtccatcat ctgaccgaca tcctgcaaaa
     2341 tccgctgcgc ccattcataa atccgctctc cttcttcggt aatggtgacg cggcgggtgg
     2401 tgcggtgtaa caacaccacg tttagcgttt gctccagcaa ggcgatgcgc ttgctgacga
     2461 acgccggtga aacgcccagt tcttccgcca cggcggcaaa accagcccga cgagccacca
     2521 gcataaagac gcgcaaatca ttcagcagcg gtaaattatt catgattcgt gttttatgtt
     2581 tcaccagtta cggggattaa ttccttttca gtcaattata ggatggtgat gttgtcaatt
     2641 ttgatggtca ggaagtgaga acccaatgat gaaaacgatg cctattgctg cgatcccggg
     2701 agacgggatt ggcaaagaag tccttcctga agggattcgc gtgttacagg ctgccgctga
     2761 gcgctggggc ttcgccttga gttttgagca aatggagtgg gcgagctgcg agtattacag
     2821 ccatcacggt aaaatgatgc cggacgactg gcatgagcaa cttagccgtt tcgacgccat
     2881 ctattttggt gccgtcggct ggccggacac cgttcctgac catatttcgt tgtggggttc
     2941 gctgctgaaa tttcgtcgtg aattcgacca gtacgtcaac ctgcgcccgg ttcgtctctt
     3001 tcctggcgtt ccctgcccgc tggcgggaaa acagcctggc gacatcgatt tttacgtggt
     3061 cagggaaaac accgaaggcg aatattcctc gctcggcggt agagtgaatg aaggtacaga
     3121 gcatgaagtc gtcattcagg aatcggtatt tacgcgtcgt ggtgtcgatc gcattttgcg
     3181 ttatgccttc gaacttgcgc aaagccgccc acgtaagacg ctaacttctg ccactaaatc
     3241 aaacggttta gccatcagca tgccgtactg ggatgagcga gtggaagcaa tggccgagaa
     3301 ttacccggag atccgctggg acaagcagca tattgatatt ctctgcgcgc gttttgtgat
     3361 gcagccggaa cgcttcgatg tggtggtggc gtccaattta tttggcgata tcctttccga
     3421 tcttggcccg gcctgcaccg gcaccattgg cattgcccca tccgccaacc tgaatccgga
     3481 acgcactttc ccgtcgctct tcgagccagt ccacggttcc gcgccggata tctacgggaa
     3541 aaacattgct aaccctatcg ccacgatttg ggccggtgca atgatgctcg attttctcgg
     3601 caatggcgat gagcgtttcc agcaagcgca taacggtatt ctggcagcaa ttgaagaagt
     3661 gattgctcac gggccgaaaa cacctgatat gaaaggcaat gccaccacgc cacaggttgc
     3721 cgacgcgatt tgcaaaatta ttttgcgtta aggtcaaacc agtttatttg aaccgcgtca
     3781 ctgacgcggt ttttttattc gttctttgca gtaaataacc tgcgtcattt caccttttat
     3841 tgtttccgtt tcgtgtttta tggctttccg tattcttaat tgtttaattt atgtaacatg
     3901 caaataatca atttgttaaa cacaacatac aactcattac cctgcaaata attacaactg
     3961 gcaaaagcca gagacgggct tttatgccca ggagatgact catgatgagc aatgttaaga
     4021 aaaaagatgt gccgctgata agcatcagcc tggtggccat tcttttcatc gcagctgcat
     4081 taagcctttt cccacaacaa tcggccgacg cggccaacgc aatatacact tttgttacgc
     4141 gtacgttagg ttccgccgta caggtattgg ttttgctggc aatgggactg gtgatttatt
     4201 tagccaccag taaatacggc aatattcgtc ttggcgaagg aaaaccggaa tacagcacgc
     4261 tctcctggct gtttatgttt atttgtgccg gtttaggttc ttctacgctt tattgggggg
     4321 ttgctgaatg ggcctattat tatcaaacgc ctggattaaa tatcgcaccg cgttcacaac
     4381 aggcactcga atttagcgtc ccttactcct tcttccactg gggcatcagc gcctgggcaa
     4441 cttatacgct ggcctcatta atcatggctt atcactttca tgtgcgtaaa aacaaaggtc
     4501 tgagtctttc cggcattatt gccgcgatta ccggcgttcg cccgcaaggc ccatggggaa
     4561 aactggttga tttgatgttc ctgatcgcca ctgtcggcgc actgactatt tcccttgttg
     4621 tcaccgcagc aacctttact cgtggacttt ccgcgctgac cggtttaccc gataacttca
     4681 ccgtgcaggc atttgtgatc ctgctttccg gcggcatttt ttgcctaagc tcgtggattg
     4741 gtatcaacaa cggtttgcaa cgtctgagca aaatggttgg ctggggcgcg ttcctgctgc
     4801 cattactggt gctgattgtc ggcccaaccg aatttattac caacagcatc atcaatgcca
     4861 tcggtctgac cacgcaaaac ttcctgcaaa tgagcttatt caccgatccg cttggcgatg
     4921 gttcatttac ccgcaactgg accgttttct actggctgtg gtggatctca tacacccctg
     4981 gcgtagcaat gtttgtcacc cgcgtttccc gcggtcgtaa gattaaagaa gttatctggg
     5041 gactgatcct cggcagcacc gtcggttgct ggttcttctt cggcgtaatg gaaagctatg
     5101 ccattcatca gtttatcaat ggcgtaatca acgtcccaca ggtgctggaa acactgggcg
     5161 gcgaaacagc tgtacagcaa gttctgatgt cgttgccagc cggtaaattg ttcctcgccg
     5221 catacctggg cgtgatgatt attttccttg cctcgcatat ggatgcggtg gcctacacca
     5281 tggctgcgac cagtacgcgt aatctccagg aaggtgacga tcctgaccgt gggctgcgtc
     5341 ttttctggtg cgtggtgatc actctgatcc cgctttccat cttgtttacc ggtgcttcgc
     5401 tggaaacgat gaaaaccacc gtcgtgctca cagcccttcc cttcctcgtc attttactgg
     5461 tgaaagtcgg cggatttatt cgctggctga aacaggatta cgccgacatt ccggctcatc
     5521 aagttgaaca ttatctcccg cagacaccgg ttgaagccct ggaaaaaaca ccagtgctcc
     5581 ctgcgggaac cgtattcaaa ggcgacaact gagcgccatc atcctaacga taaaggtatc
     5641 cctatgagca atctgagccc tgactttgta ctacccgaaa atttttgcgc taacccgcaa
     5701 gaggcgtgga ccattcctgc ccgtttttat accgatcaga acgcgtttga acacgaaaaa
     5761 gagaacgtct tcgccaaaag ctggatttgc gtcgctcaca gcagcgaact ggcgaatgcc
     5821 aatgattatg tgacgcgtga gatcattggc gaaagcatcg tgctggtacg cggtcgtgat
     5881 aaggttttgc gcgcgttcta taacgtgtgt ccgcaccgtg gtcatcagtt gttgagcggt
     5941 gaaggaaaag caaaaaatgt gattacctgc ccgtatcacg catgggcatt caaactcgat
     6001 ggcaatctgg cccatgcacg taactgcgaa aacgtcgcca atttcgatag cgacaaagcg
     6061 caactggttc cggtgcgtct ggaagaatat gccggattcg tcttcatcaa catggacccc
     6121 aacgccacca gcgtagaaga tcagttaccc ggtctgggcg cgaaagtgct ggaagcctgc
     6181 ccggaagtcc acgatctgaa actggcggcc cgctttacca cccgcacgcc tgccaactgg
     6241 aagaacattg tcgataacta tctcgagtgc tatcactgtg gtccggcgca tccaggtttc
     6301 tccgactccg tacaggttga tcgttactgg cacaccatgc acggtaactg gacgctgcaa
     6361 tacggtttcg ccaaaccgtc cgaacagtcg tttaaatttg aagagggtac ggatgcggca
     6421 ttccacggtt tctggctgtg gccgtgcacg atgctgaacg tcaccccgat caaagggatg
     6481 atgacggtca tttatgaatt cccggtggat tctgaaacta ccctgcaaaa ctacgatatt
     6541 tacttcacca atgaagagtt aaccgacgag caaaaatcgc tgattgagtg gtatcgcgat
     6601 gtgttccgtc cggaagattt acgtctggtt gaaagcgtac agaaagggct gaaatcgcgt
     6661 ggctatcgtg gtcaggggcg catcatggcc gacagtagcg gtagtggcat ttccgaacat
     6721 ggtatcgccc atttccataa tctgctggcg caggtgttta aggactaatg acatcggcgg
     6781 cggtattttc cgccgctggg ctgatttttg atggagtaca gcaatgtcag actatcaaat
     6841 gtttgaagta caggtgagtc aggtggaacc gcttaccgaa caggtgaaac gcttcacgct
     6901 ggtggcaacc gatggcaaac cgctgcctgc gttcaccgga ggaagtcaca tcattgtgca
     6961 gatgagcgat ggtgataacc agtacagcaa tgcgtattca ctactgagtt cgccgcatga
     7021 cacctcttgt tatcagattg ccgttcggct ggaggaaaac tcgcgcggcg gttcccgctt
     7081 tttgcatcag caggtaaaag tgggcgatcg gttaacgatt tcaacgccta ataacctgtt
     7141 tgcgctaatt ccctcagcca gaaagcatct gtttatcgcg ggcggtattg gtatcacccc
     7201 tttcctgtcg cacatggcag agctgcaaca cagcgacgtc gactggcagc tacattactg
     7261 ctcgcgaaat ccagaaagtt gcgcatttcg tgatgagcta gtccagcatc cgcaggctga
     7321 gaaagtccat ttgcatcatt catcaaccgg aacacgactg gaattagcgc gattattggc
     7381 ggatatcgaa cctggcacac acgtttatac ctgtggcccc gaggcgctaa ttgaagcggt
     7441 aagaagtgaa gctgcgcgtc tggacatcgc cgccgatacg ctgcactttg agcaatttgc
     7501 tatcgaagac aaaaccggcg atgcatttac cctggtgctt gcccgttccg gaaaagagtt
     7561 tgtggtgccg gaagagatga ctattttgca ggttattgaa aataataaag ccgcaaaagt
     7621 ggaatgttta tgtcgtgaag gggtatgcgg aacctgcgaa acagcaatac tggaaggtga
     7681 agctgaccat cgggatcaat attttagcga tgaagagcgt gccagccagc aaagtatgtt
     7741 gatctgttgt tcgcgtgcga agggtaaacg cctggtgttg gatttgtagt ttgccgaagc
     7801 cggatgtggc gctgagcgcg cccagtccgg cttcggaaga ttttactgcg gatattcctg
     7861 caataaattg tgtaatgctt ccgccatcag ctcaccacgc cagccggaaa tcagctccgg
     7921 caaattgttc tgcggtttca gtttccagtg ccagttcagc agttggttga tttgccgacg
     7981 cgatgccagc aattcggcgc tgatcttatg cgtttcgctc acgtcagtaa tcagcgactt
     8041 aatcgcttta aacgctttac gataacccgg catgtccatc aggttaagca tcggctgcgg
     8101 taaggcctct tccggcaatg tctgcgcttt ttccaccagc gccagcagcg ttttaccgtg
     8161 aaagcggatt tcgctaccgg ataaacccag gctgtccagt tcgcctaaac ttcccggcat
     8221 ataacgcgct accgaccaca aatgctcttc acgcacgaca aagttcaccg ccagatcgcg
     8281 ctctcgcgcc ttgcgcagtc gccagtcggc taacagttgc agacaggcca gttggcgtgt
     8341 gcgtaattgc caggcattgg tgatatcacg ccaggcatct tccggcgcaa cgacttcctg
     8401 acgacgcatt tgcatcaggc ggcattcatc cagcgccgca ggtagccagc cggaggcctc
     8461 cgtttctacc ataagcttgg cggtgatcgg taacagatac cagacatccg ccgctgcgta
     8521 ttcacactga cgttcggtca gcggtctggc cagccagtcg gtgcgcgatt cactcttgtc
     8581 cagcgtaacg ccggaatact cttccaccat ggaagcgaaa ccccatgaca tcgggcgtcc
     8641 gcagaaggca gcaaggattt gcgtgtcaat caagggttgt ggtaattcgc caaagacatt
     8701 gaggaacact tccagatctt cactgcctgc atggagaaat tttgtgatgg acggatcgcg
     8761 caggatcgct ttcagcggtg accagtcggt gatcccgaga ggatcaatta gcgccagatg
     8821 ctcgccatcg aaaagttgaa tcaaccccag ctgcgggtaa taagtgcgcg tacgaacaaa
     8881 ttcagtatcc agggctatcg ccggaaaggc acggacggct tcacacaaag aagccagcgc
     8941 atcgtccgtg gtaatcattt ggtaattcaa attgttttct ctttagtggg cgtcaaaaaa
     9001 aacgccggat taaccggcgt ctgacgactg acttaacact caggctttat tgtccacttt
     9061 gccgcgcgct tcgtcacgta attctcgtcg caaaattttt ccgacgttag atttcggtaa
     9121 ctcatcacga aactccacca gcttcggtac tttgtagccc gtgagctgac ggcggcaaaa
     9181 ggtcaccagt gactcttcgg taagcgatgg atcttttttc actacgaaga ttttcaccgc
     9241 ttcaccactg gagccggaag gtacgccaac agccgcgact tcctgtacgc caggatgctg
     9301 catgacgaca tcttcaatct cgttgggata gacgttaaaa ccggaaacca gaatcatgtc
     9361 ttttttacga tcgacaatgc gcaggaaccc ttcttcatcc atcaccgcaa tgtcgccggt
     9421 gtgtaaccag ccatttttga tgatctcatc tgtagcatcc ggacgctgcc agtaacccag
     9481 catcacctgc ggtcctttga cacaaagctc acccggttga cccggtggta cttcattatc
     9541 atcatcatcc accagtttgg cttccgtcga cggcaccggc aaaccgatgc taccactatg
     9601 ataatcaata tcatatgggt taacgctgac cagcggcgca cactcggtaa ggccatagcc
     9661 ttccagcaga tactgtcctg tcagtttcac ccaacgctct gccaccactt gctgcactgg
     9721 catccctccg cctgcggaaa gatgcagact ggagaaatcc agctgctgga actctttatt
     9781 gttcagcaac gcattgaaca aggtgttaac gcccgtgata gcggtaaacg gatatttcgc
     9841 taactctttt accaaccctg gaatatcgcg cgggttagtg ataagcaggt tctgcccacc
     9901 cagttcgata aacagcaggc agttaatggt cagggcaaaa atgtgataca gcggcagcgc
     9961 cgtcaccacc agctctttgc ccggatgcaa cagcggacca taggtggcgt tgacctgttc
    10021 caggttcgcc agcatattgc ggtgagtcag catcgcgcct ttcgccacac ctgtggtgcc
    10081 gccggtgtat tgcaggaagg ctaaatcttc cggcaccagt tcgggtttga cgtactgcat
    10141 ccggtagccg ttatgcagtg cgctacgaaa tgaaatggca tctggcagat ggtatttcgg
    10201 caccaaacgc ttgatgtatt taacaacgaa attgactacc gtgccttttg ccgtagatag
    10261 ctgatcgccc atacgggtca gaattacgtg ctgaacggcg gttttatcaa ccactttttc
    10321 cagtgtgtga gcaaagttag acacgataac aatcgccgat gcgccgctat cgttaagctg
    10381 atgctcaagc tcacgcgggg tatacaacgg gttaacgttt acgacgatca tcccggcacg
    10441 caaaatgcca aacagcgcca ccggatattg caataaatta ggcatcatca acgcaacgcg
    10501 atcgcctttc ttcagcccca acccttgttg caaataagcg gcaaacgcgc gactgcgttc
    10561 ttccagcttg cggaaggtca ttacctcccc catattcaca aacgcaggct gatcggcgta
    10621 gcgcgcgacc gactgctcaa acatatctac cagagattga taacggtcag ggttgatctc
    10681 cgtcggaacg tccgcgggat aacggttaag ccaaaccttc ttcaattctt cacctctaaa
    10741 atgcgtgttc gtcgtcatcg caaccccaaa tgatatacat gccgttaaca taatattaac
    10801 tcatcatacc agcttgataa ttacccaacg aaaaggttgc gaagcgcgtc actatttatt
    10861 tttatcttta ccgtaagaat gcagaaacag cggaccagcc gctgtttctt tttctttaaa
    10921 aacaagcaat tattcagtta caactgtttg tactctcgcg gggccgggat tataccagcc
    10981 ccatccgcca tagccatagg gccagccacg accgccataa aaccatggat caatcggctg
    11041 aggcggcata atcacctgct gggttaaatg ccaacgtttg taacccgtta cttgcatcac
    11101 cataaattta tagggcgtgt tgccgatttt tccgtcaacc gcaccagtga ttggcccgac
    11161 taccgtaacc agttgtccac ggaaatccac cgggtccaga aaaccgttca tatcggcata
    11221 aatgcgaccg cgagaaggtt ctcccagcgt cggtctggct ccgctgtcca gcggtacggt
    11281 agcaatttcc aggcgggttt tcccttgctg gttttgtacc gcaaccactt tgccaccaaa
    11341 gcgtgcctcc tgaccaacgt acagctgcgg cgcactcatc acccgaacta aatcttgttg
    11401 cggcgtggga ctgctgcctt taatggcgtc cggcacagtg acacaaccgc tcagcattag
    11461 cgcaaacgta cctgccagta tgcctttgat aacattcttt tgaaccgcca tggtgcgact
    11521 cctttttttc tcagggcata ctcttaagat tcattctttg cccggaagtt tcttccatgc
    11581 gacgttgtta cgtaaataaa ccggttcggc atgttccacc gccaccgttt taccctcggc
    11641 aaacatctga cacgcaatcg gcagcatatc ttcagcagca ggcagtaaca cttcgccatc
    11701 gcgcaaaacc agcccgctct ctttaccgag atccggccag gcttgccagc ccgtccctac
    11761 cgtcacccat tcgccggaaa gctgttgcat tcgttcatgg acgatttcgg gtttgagtac
    11821 ggcttcggtt tcttcaccgt gccagatacc gttttcatca cgctgatatt cggcccagta
    11881 aacttcgccc attcgcgcgt caatggctga cagcacgcgg gttgcgccgt ttttgcgcca
    11941 cgccccttgc gccatcgtca ttagcgtgga gacgccaatc atcggtaatt ccgcgccaag
    12001 cgccagccct tgcgcgatgc caataccaat gcgcacgccg gtaaagctac cggggccgcg
    12061 cccgtaagcc agagcgttta tgtcagtcag ggaagttccg ctggtggtca ggatatcctg
    12121 caccatcggt aagattcgtt gagtatgttc acgagggcaa agctcaaaat gagcgttgac
    12181 agtaccgtcg ttccacaggg cgacagagca ggcctctgtc gcggtatcga tagccagaat
    12241 tcgcatgggt cttcgtgctt ttttcaataa aaaggcgcgc atcataccat actccgtaac
    12301 aaattacctg gaggatggta tcgcaaggaa acgaaccgca cgggcaatgt cacgggtgcg
    12361 cggcgcgggc ggcagactgg cgagaaacgt cgcgccgtaa gggcgcatca ccagccgatt
    12421 gtcacaaatc accaaaaccc cgcgatcgtc ggcgtcgcga atcagtcgcc ctaccccctg
    12481 cttgagagta atcaccgcat ccggcagttg tacttcatcg aacgggtcgc caccgcgcaa
    12541 acggcaatct tccatgcgcg cttttaacag cgggtcatcc ggtgaggtaa acggcaactt
    12601 gtcgataatc accaatgaca atgtatcgcc acgcacgtcc accccttccc agaaactact
    12661 ggttgccaca agaagcgcgt taccggcgct gacaaattgc tgcaacagtt gccctttgct
    12721 ggtttccccc tgcaacaaaa cgggaagcgt catggtagcg cggaactgct cggccagatc
    12781 acgcatcatg gcgtgcgagg tacaaagcat aaaacaacga ccgttgttag cttcgatgat
    12841 cggtcgcagc attgccgcta actggcgagc agaacctggc tggttggttt gcggcagatt
    12901 gcgcggcaca cagagtaacg cctggcggct gtaatcaaac gggcttggca atagcaacga
    12961 ctccgcctgt tctatcccaa gccgcgaggt gaaatgatgc agatcgtcgt tcaccgacag
    13021 cgttgctgag gtgaagatcc agctaccagg cttttgcgcc attaactctt tgaatttatc
    13081 cgccacgctg agcggcgtga gagccagagt aaaatggcgc gaagtgcatt cgtaccagta
    13141 gctgtagccc ggctgattga tctcttttag ccgcttcagc cgtgtgcgat acaacgtagc
    13201 gcgctcaaat gccgcatcca gcaaggcgga acgccccagt gacagtttcg ccacgtcata
    13261 acaaagttcc agggtgtcat cgagcagtaa aaatgcccgc tgaatttgcg gattagctaa
    13321 cagctcacgc aggttaccgc gataacctgg ctcaccgagt tgcagacgaa aatcctgcgc
    13381 actctgggca agacgatcag cgcacttttg taactgctgg gtgtctttta attcggtgcg
    13441 gtaagcgatg gtgatgtctt ttgccaggtc gagcagttgt cgactggaga gtgactgacc
    13501 aaaatactgg ctggcaatgt ccggtagctg gtgggcttcg tcgaagatca tgacgtccgc
    13561 ttccgggatc agttcgccaa atccactctc tttaaccacc atatccgcca gaaagagatg
    13621 atggtttacc accaccacat cggcgtccat cgctttttta cgtgctttga ccacaaagca
    13681 atctttatac atcgggcagt cgctgccaag acagttgtcg ttggtgctgg tgaccagcgg
    13741 ccacgcctgt gaatcttccg ccacgctgac gcaggtgctg atatcaccat cgactgtttg
    13801 attagaccag gagcgcagca ggatcacatc gcttaagatt tgtaccggca gatcgccccc
    13861 cgccagcgcc tgctgttcga gacgttcgag gcagaggtag tttgagcgcc ctttcagcag
    13921 cgccacgttg cccgtatatt tcaatgcctt tgagactgtt ggcaaatcgc ggctgtagag
    13981 ctgatcctgc aacgcttttg agccggtcga gataatgact ttctttttcg cccgcagcgc
    14041 aggagccagg taagcgtagg ttttgcccgt accggttcct gcttccacca ccagcggctg
    14101 gcctttttct atcgcctggg tgacggctac cgccatctgt cgctgtggtt ctcgcggctt
    14161 aaagcctggt atcgctttcg ccagctgacc gtctggtgca aaatcgtccg tcacactacc
    14221 ccctgttgat ttgaacaggg attatgtcag gatgagggcg ctttcgccag ttgaagtggt
    14281 gacggcgacc tcacattgtg gcagtctttg cagcacgaaa atggaaggat aatgaggaca
    14341 aaatgatgac tatcgttcgt atcgatgctg aagcccgctg gtctgatgtg gtaatccaca
    14401 acaacacgct ctactacact ggtgtaccgg aaaacctcga cgccgatgcc tttgagcaaa
    14461 ccgccaacac gctggcgcag attgatgccg tgctggaaaa acagggcagc aataaatcga
    14521 gcattctgga tgccaccatt ttcctggccg ataaaaacga cttcgcggcg atgaataaag
    14581 cgtgggatgc ctgggttgtc gcgggtcatg cgccggtgcg ctgcacggta caagcgggtt
    14641 tgatgaaccc gaagtataaa gttgaaatta agattgtggc tgcggtgtaa gctttatcga
    14701 agcaaaataa gtcagacgat aatttatcga taatactggt cggttttaca taaatcgacc
    14761 agagattaag atattacctg ttgatgctcc tgctaattca ttatttatt
//
LOCUS       DAZFQD010000131        14729 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.131, whole genome shotgun sequence.
ACCESSION   DAZFQD010000131 DAZFQD010000000
VERSION     DAZFQD010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14729
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.131"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(16..168)
                     /gene="hokE"
                     /locus_tag="ACHKTW_004019"
     CDS             complement(16..168)
                     /gene="hokE"
                     /locus_tag="ACHKTW_004019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HFO6464944.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            137..406
                     /locus_tag="ACHKTW_004020"
     CDS             137..406
                     /locus_tag="ACHKTW_004020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6464945.1"
                     /translation="MTATRAYFVSMFILPPCEEGTTIQIVQVWVGSPRVDRNIVLGLH
                     LSRPQSKPETGKLQAPAVILPLLSKIAVYLYSKSCFGLLTPHLIN"
     gene            610..1728
                     /locus_tag="ACHKTW_004021"
     CDS             610..1728
                     /locus_tag="ACHKTW_004021"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbdK family carboxylate-amine ligase"
                     /protein_id="HFO6464946.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNK
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTHPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFTSSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRPLTEDTLRLLEKIAPSAHKIGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWAGD"
     gene            1794..2042
                     /locus_tag="ACHKTW_004022"
     CDS             1794..2042
                     /locus_tag="ACHKTW_004022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HFO6464947.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVTMFHL
                     MDLSQLYTLLFCLWFLVLGAIEYFVLRFIWRRWFSLAD"
     gene            2107..2475
                     /locus_tag="ACHKTW_004023"
     CDS             2107..2475
                     /locus_tag="ACHKTW_004023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFO6464948.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYPGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            2569..3222
                     /gene="nfsB"
                     /locus_tag="ACHKTW_004024"
     CDS             2569..3222
                     /gene="nfsB"
                     /locus_tag="ACHKTW_004024"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HFO6464949.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNERKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNITLTEV"
     gene            3330..4577
                     /gene="ybdG"
                     /locus_tag="ACHKTW_004025"
     CDS             3330..4577
                     /gene="ybdG"
                     /locus_tag="ACHKTW_004025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HFO6464950.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESILNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(4645..6021)
                     /gene="pheP"
                     /locus_tag="ACHKTW_004026"
     CDS             complement(4645..6021)
                     /gene="pheP"
                     /locus_tag="ACHKTW_004026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HFO6464951.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGHGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(6123..9266)
                     /gene="cusA"
                     /locus_tag="ACHKTW_004027"
     CDS             complement(6123..9266)
                     /gene="cusA"
                     /locus_tag="ACHKTW_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HFO6464952.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKASENGVPVYLRDVAKVQIGPEMRRGIA
                     ELNGEGEVAGGVVILRSGKNAREVIAAVKDKLETLKSSLPEGVEIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVAVVCALFLWHVRSALVAIISLPLGLCIAFIVMHFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKRLEEWQHQHPDATLDNKTRWQVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGK
                     IPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQIN
                     EGDLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEMV
                     ETTIQLKPQEQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLADIDAMAEQIEEVARTVPGVASALAERLEGGRYINVEINREKAARYGMTV
                     ADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITL
                     ADVADIKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSV
                     AFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWL
                     LWWMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAEPSLNNPQTFSEQKLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLMWLHRHRVRK"
     gene            complement(9278..10501)
                     /gene="cusB"
                     /locus_tag="ACHKTW_004028"
     CDS             complement(9278..10501)
                     /gene="cusB"
                     /locus_tag="ACHKTW_004028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
                     /protein_id="HFO6464953.1"
                     /translation="MKKIALIIGSMIAGGIISAAGFTWFAKAEPPAEKTSTAERKILF
                     WYDPMYPNTRFDKPGKSPFMDMDLVPKYADEESSASGVRIDPTQTQNLGVKTATVTRG
                     PLTFAQSFPANVSYNEYQYAIVQARAAGFIDKVYPLTVGDKVQKGTPLLDLTIPDWVE
                     AQSEYLLLRETGGTATQTEGILERLRLAGMPEADIRRLIATQKIQTRFTLKAPIDGVI
                     TAFDLRAGMNIAKDNVVAKIQGMDPVWVTAAIPESIAWLVKDASQFTLTVPARPDKTL
                     TIRKWTLLPGVDAATRTLQLRLEVDNADEALKPGMNAWLQLNTASEPMLLIPSQALID
                     TGSEQRVITVDADGRFVPKRVAVFQASQGVTALRSGLAEGEKVVSSGLFLIDSEANIS
                     GALERMRSESATHAH"
     gene            complement(10517..10849)
                     /gene="cusF"
                     /locus_tag="ACHKTW_004029"
     CDS             complement(10517..10849)
                     /gene="cusF"
                     /locus_tag="ACHKTW_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HFO6464954.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEAQPQVISATGEV
                     KGIDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            complement(10873..12255)
                     /gene="cusC"
                     /locus_tag="ACHKTW_004030"
     CDS             complement(10873..12255)
                     /gene="cusC"
                     /locus_tag="ACHKTW_004030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HFO6464955.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRPAMPVPQQFSLSQNGLVN
                     AADNYQNAGWRTFFVDNQVKTLISEALVNNRDLRMATLKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGNTATTREFSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSDSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISTASSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLNDQIS
                     AQQRYLASLQITLQRARALYQHGAVSYLEVLDAERSLFATRQTLLDLNYARQVNEISL
                     YTALGGGWQQ"
     gene            12412..13095
                     /gene="cusR"
                     /locus_tag="ACHKTW_004031"
     CDS             12412..13095
                     /gene="cusR"
                     /locus_tag="ACHKTW_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HFO6464956.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLMKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            13085..14533
                     /gene="cusS"
                     /locus_tag="ACHKTW_004032"
     CDS             13085..14533
                     /gene="cusS"
                     /locus_tag="ACHKTW_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HFO6464957.1"
                     /translation="MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFV
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYNSP
                     GAPDIREFTRDAIPDKDAQGGEVYLLSGPTIMMPGHGHGHMEHSNWRMINLPVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTVPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELRFVGDECQVAGDPLMLRRALSNLLSNALRYTP
                     PSEAIVVRCQTVNHQVQVSVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFVIMLPERE"
BASE COUNT         3533 a         3974 c         3634 g         3588 t
ORIGIN      
        1 ctccccgcac aacggctact tcttcggttc gtaagcgagt acagccttaa actcaatatt
       61 acgttccttt acggtgaact cacacagcga gtctccgacc agaagcgtaa atcccagtac
      121 cgtcaaacac agcactatga ctgccacaag ggcatatttc gtcagcatgt ttatattgcc
      181 tccatgcgaa gaagggacta ccatccaaat tgttcaggtt tgggtaggta gccccagggt
      241 tgatagaaat atcgtcctgg ggcttcatct ttccagacct cagagtaaac ctgaaaccgg
      301 aaagcttcag gcacccgcag ttatcttacc cttgcttagc aaaatagctg tttatttata
      361 cagcaagtcc tgtttcggac ttctcactcc ccatttgatc aactgaagct gaacccgttt
      421 ttttctgcat gcctcaatcg tcaaaattct tcgccaccag ccagccgtgc cataacttgt
      481 cacattgcaa aagtagccaa attgcttcag tcaccatcac caaaaaaaga aacattccag
      541 aacttccgca tctcgcccag ccgctccgac ttctatactg aatagaaaac gccaacttaa
      601 gagaaaccta tgccattacc cgattttcat gtttctgaac cttttaccct cggtattgaa
      661 ctggaaatgc aggtggttaa tccgccgggc tatgacttaa gccaggactc ttcaatgctg
      721 attgacgcgg ttaaaaataa gatcacggcc ggagaggtaa agcacgatat caccgaaagt
      781 atgctggagc tggcgacgga tgtttgccgt gatatcaacc aggctgccgg gcaattttca
      841 gcgatgcaga aagtcgtatt gcaggcagcc gcagaccatc atctggaaat ttgcggcggt
      901 ggcacacacc cgtttcagaa atggcagcga caggaggtat gcgataacga gcgctatcaa
      961 cgaacgctgg aaaactttgg ttatctcatt caacaggcga ccgtttttgg tcagcatgtc
     1021 catgttggct gcgccagtgg cgatgacgcc atttatttgc tgcacggcct gtcacggttt
     1081 gtgccgcact tcatcgccct ttctgccgcg tcgccgtata tgcagggaac ggatacgcgt
     1141 tttacctcct cacggccgaa tattttttcc gcctttcctg ataatggccc gatgccgtgg
     1201 gtcagtaact ggcaacaatt tgaagccctg tttcgctgtc tgagttacac cacgatgatc
     1261 gacagcatta aagatctgca ctgggatatt cgccccagtc ctcattttgg cacggtggag
     1321 gttcgggtga tggatacccc gttaaccctt agccacgcgg taaatatggc gggattaatt
     1381 caggctaccg cccactggtt actgacagaa cgcccgttca aacatcagga gaaagattac
     1441 ctgctgtata aattcaaccg tttccaggcc tgtcgctatg ggctggaagg cgtcattacc
     1501 gatccgcaca ctggcgatcg tcgaccgcta acggaagaca ccttgcgatt gctggaaaaa
     1561 atcgcccctt ccgcacataa aattggtgca tcgagcgcga ttgaggccct gcatcgccag
     1621 gtcgtcagcg gtctgaatga agcgcagctg atgcgcgatt tcgtcgccga tggtggctcg
     1681 ctgattgggc tggtgaaaaa gcattgtgag atctgggccg gtgactaagt agggaattgc
     1741 cttacagaag aatatccctg acaatggtgt tttaacttac atttaacaat gttatgaaac
     1801 accctttaga aaccttgact accgcagcag gcattttgct gatggctttc ctctcttgcc
     1861 tgctgctgcc cgcccccgca ctggggctga cgctggcaca aaaactggtg accatgttcc
     1921 atctgatgga tcttagccag ctttacactt tattgttttg tctgtggttt ttagtgctgg
     1981 gcgctattga gtattttgtt ctgcgcttta tctggcgacg ctggttctcg ctggcggatt
     2041 aaacgtcgat tatcgccgta cttcgcggca tactttgctt attctccttc gcctaaagga
     2101 acgtttatgg ataagcaatc actgcacgaa acggcgaaac gcctggccct tgagttaccc
     2161 tttgtcgagc tttgctggcc ttttggcccg gagttcgatg tttttaaaat tggcggcaaa
     2221 atttttatgc tgtcgtcgga gctgcgcggc gtccccttta tcaatctgaa gtccgatcca
     2281 caaaaatccc tgttaaatca gcagatatac ccgagcatta agccaggata tcacatgaat
     2341 aaaaagcact ggatttcggt gtatcccggc gaggaaatct ccgaagcgtt acttcgcgat
     2401 ctgatcaacg attcgtggaa tctggtggtt gatggtctgg ctaaacgcga tcaaaaaaga
     2461 gtgcgtccag gctaaagcgg aaatctatag cgcatttttc tcgcttacca tttctcgttg
     2521 aaccttgtaa tctgctggca cgcaaaatta ctttcacatg gagtctttat ggatatcatt
     2581 tctgtcgcct taaagcgtca ttccactaag gcatttgatg ccagtaaaaa acttaccccg
     2641 gaacaggccg agcagatcaa aactctcctg caatacagcc catccagcac caactcccag
     2701 ccgtggcatt ttattgttgc cagcacggaa gaaggtaaag cgcgtgttgc caaatccgct
     2761 gccggtaatt atgtgttcaa cgaacgcaaa atacttgatg cctcgcacgt cgtggtgttc
     2821 tgtgcaaaaa ccgcgatgga cgatgcctgg ctgaagctgg ttgttgacca ggaagatgct
     2881 gatggccgct ttgccacgcc ggaagcgaaa gccgcgaacg ataaaggtcg caagttcttc
     2941 gctgatatgc accgtaaaga tctgcatgat gatgcagagt ggatggcaaa acaggtttat
     3001 ctcaacgtcg gtaacttcct gctcggcgtg gcggcgctgg gtctggacgc ggtacccatc
     3061 gaaggttttg acgccgccat cctcgatgca gaatttggtc tgaaagagaa aggctacacc
     3121 agtctggtgg ttgttccggt aggtcatcac agcgttgaag attttaacgc tacgctgccg
     3181 aaatcacgtc tgccgcaaaa catcacctta accgaagtgt aattctctct tgccgggcat
     3241 ctgcccggct atttcccctc agattctcct gatttgcata accctgtttc agccgtcatc
     3301 ataggctgct gttgtataaa ggagacgtta tgcaggattt aatatcccag gttgaagatt
     3361 tagcgggtat tgagatcgat cacaccacct cgatggtgat gattttcggt attatttttc
     3421 tgaccgccgt cgtggtgcat attattttgc attgggtggt actgcggacc ttcgaaaaac
     3481 gtgccatcgc cagttcacgg ctttggttgc aaatcattac ccagaataaa ctcttccacc
     3541 gtttagcttt taccctgcag gggattatcg tcaatattca ggcggtattc tggctgcaaa
     3601 aaggcaccga agcggcagat attctgacta cctgcgcgca gttgtggatc atgatgtatg
     3661 cgctgctttc agtcttctcg ttgctggatg ttattttgaa tctggcgcag aaattcccgg
     3721 cggcatctca gttaccgctg aaagggatat ttcaggggat taaactgatc ggcgcaatcc
     3781 tggtcggcat tttgatgatc tcgctgctga ttggtcagtc gccagcgatt ctgatcagcg
     3841 gtcttggtgc aatggctgcc gtgctgatgt tggtttttaa agatccgatt cttggtctgg
     3901 tggcaggtat tcagctttcc gcgaacgata tgctgaaact gggcgactgg ctggagatgc
     3961 cgaaatacgg cgcggatggc gcggtgatcg atattgggtt aaccaccgtc aaagtgcgta
     4021 actgggacaa tacgattacc actattccca cctggtctct ggtttctgac tcctttaaaa
     4081 actggagcgg gatgtcagca tctggcgggc gacgtattaa gcgcagtatc agtattgatg
     4141 tcaccagtat tcgttttctt gatgaagacg aaatgcaacg tctgaataaa gcgcacttgt
     4201 taaaacctta tttaaccagc cgccatcagg aaattaatga gtggaatcgc cagcaaggtt
     4261 ctacggagtc gatattaaac ctgcgccgaa tgaccaatat tggaaccttt cgtgcctatc
     4321 tgaacgaata tctgcgtaac catccgcgga ttcgtaaaga tatgacttta atggtacgcc
     4381 aactggctcc aggtgataac ggtttaccgc tcgagatcta tgcgtttacc aacaccgtgg
     4441 tgtggctgga atatgaaagc attcaggctg atatattcga tcacatattt gcgattgtcg
     4501 aagagtttgg tctgcgactt catcagtcgc caaccggcaa tgatattcgc tctctggcgg
     4561 gtgcatttaa gcagtaatta aaaaaaccgc tctcatcgaa tggatgagag cggacatcgt
     4621 tttgtcggat gtagcgtgaa tgccttattt ccgacgcagc gttttaaatg ccacaaacag
     4681 gaatacaatc cacaccggca gcaggatcgc tgacaagcgc atatcatcca tcgtgcacat
     4741 cagcagcaaa atcatgccga ggaaggcaat gcaaagatag ttgccgaacg gatagagcag
     4801 cgccttaaac tgtgtttcac gcccctgacg tcgcatcgct gcacgaaaac gcagatgcgc
     4861 caggcagatc ataatccagt tcaacagcag cgttgctacc accagcgcca tcagcagacc
     4921 aaacgctttt tgcggcagca gatagttgat taataccacc agcgaagtga tcgctccgga
     4981 aagcatcagc gaattaatcg gcacaccgcg acggctgacg cgagtcaaaa acttcggcgc
     5041 attaccctgc acagaaaggc caaacagcat gcggctgtta gagtaaaccc cgctgttata
     5101 cactgacagc gatgctacca gaatgacgaa gttcagcgca gaagctacca cgttgctgtc
     5161 gagattatgg aaaatcatca caaacgggct actgttggat ttcacttcca cccacggata
     5221 gagcgccagt aaaaccacca gtgagccgat gtaaaacagc aggatgcgat acaccacctg
     5281 atttaccgct tttggaatgc ttttttccgg atcgcgcgct tcagcggcag taatcccaat
     5341 cagctccaga ccgccgaagg agaacataat taccgccagc gacaaaatca gcccattcca
     5401 gccggtggca aagaaaccac cgtagcgcca gaggttgtcg atactggctt tctcgccgcc
     5461 gtgaccagaa aacagcagcc acaggccaaa gccgatcata ccgatgatcg ccagcacttt
     5521 aatcaacgca aaccagaact cggtttcgcc atataagcgc acgttcacca ggttaacggc
     5581 gttgatgata ataaagaagg cggcagccca aatccacgtt ggaacatccg ggaaccagta
     5641 ctgcatatag atgcccgcag cggtcagctc tgccatcccc accagcacga acatcaccca
     5701 gtagttccag ccagagagga agcccgcgaa cggtccccag tatttatagg caaagtgggc
     5761 aaacgaaccg gataccggct cttcgaccac catctcgccg agctggcgca taatcaggaa
     5821 agcgatgatc ccggcgacgc cgtagcccag caatacagcc ggacccgcca tctgaatcgc
     5881 cgggccaatg ccaagaaaca gaccagtacc aattgcgcca cccagcgcaa tcagttgaat
     5941 atgacggtta tgtaatccgc gatgaagcgt cggctcttga ttcgacgcag tatcttccga
     6001 tacggttgac gcgtttttca cgcctttccc ctgtgtgtct tttttgttga ggggcacctt
     6061 ttaacattta gtgcccatcg tcgcaagaca caatccacac ggttaaacgg ggtatcctgc
     6121 ttttatttcc gtacccgatg tcggtgcagc cacatcagct tatacgccgc cgggataata
     6181 aacagcgaca gcaaaggtgc ggtgatcatg ccgccaatca tcggtgcggc aatccggctc
     6241 atcacctctg accctgctcc tgttccccac agaatcggca gcagaccagc gataatcacc
     6301 gccaccgtca tcgctttcgg gcgcacgcgc agtaccgcgc cgtgatacaa cgcctcatcc
     6361 agcttctgct cgctgaatgt ttgcggatta ttcaacgacg gctcggcctc tatggcgtga
     6421 cgtaaataca tcagcatcac cacgccaaat tcggcggcga ccccggcgag ggcgataaag
     6481 ccagtgcccg tcgccacgga aagatgaaag cccatccacc acagcaacca gatgccgccc
     6541 accagcgcaa acggtacgct gctgataatc agcaacgctt cgcccacccg acggaacgcc
     6601 agatacaaca gcacgaagat aatcatcaac gtcatcggca ccatgagttt aagcttatgg
     6661 ttggcgcgct ccagtagctc gaactgcccg gagaatgcca cgctggtgcc cggttttaac
     6721 tgcacttttt cagctatcgc tttttgcaaa tcgtgaacca ccgacaccat gtcacgatcg
     6781 cgggcatcga tataaatcca gctcgtcggg cgcgcattct cggttttcag cattgacggt
     6841 ccggtagaga ctttaatgtc ggccacgtct gccagggtga tttgctgctt catcggcgtc
     6901 agaatcggca gctggcgcag tgcctgcgga ctatcgcgcc agctttgcgg ataacgcaga
     6961 ttaattggat aacgggcaat tccttccacc gtttcgccaa ccatcgcccc gcccacagca
     7021 gaagtcacaa acaactgcac atccgccacc gtcataccgt aacgcgcggc tttttcacgg
     7081 ttaatctcaa cgttgatata gcgcccacct tccagccgct cggcaagagc agaagctacg
     7141 cctggcaccg ttcgcgccac ttcttcaatt tgctcagcca tcgcgtcgat atccgccagc
     7201 acagtgccgg aaactttaat gccgatgggg cttttaatgc cggttgagag catatcgata
     7261 cggttacgaa ttggcggcac ccacagattc gccagccccg gcagccgcac ggtgttatcc
     7321 agttcctcaa tgattttgtc catcgtcatg cctggccgcc actgctcctg cggcttaagc
     7381 tggatggtcg tttctaccat ctccagcgga gcagaatcgg tggcggtttc cgctttcccg
     7441 gttttgccaa atacccgcgc cacttcaggt acgctcataa ttagcttgtc ggttttttgc
     7501 agcatactcg ccgcctctgc tgcggaaatc cccggcagcg tcgatggcat atacaacaag
     7561 tcgccttcat tgatctgcgg taaaaattcc ccgccaactt tattgagcgg ccagagaacc
     7621 gtcagcaccg aaagcgccgc caccagcagc gtggttttcg gccagtgcag tactttcagc
     7681 aacagcggat gataaacacg aatcaaaaag cgattgagcg ggttactgct ttccggcgga
     7741 attttgccac ggatccagta gcccatcagg atcgggatca ctacgatcgc cagcagcgcc
     7801 gcacccgcca tcgcatacgt tttggtgaac gccaacgggc caaacagacg cccttcctgc
     7861 ccttccaggg tgaagatcgg gataaacgac aacgtgataa tcagcagact gataaatagc
     7921 gccggcccca cttcaacaga cgcatcggtg atcacctgcc agcgcgtttt attatccagc
     7981 gtggcgtcag gatgctggtg ctgccactct tccagccgtt tatgtgcatt ttcgatcatg
     8041 acgatagcgg catcgaccat cgccccgacg gcaatcgcaa tgccacccaa cgacataata
     8101 ttggcattca gtccctggaa gtgcatgaca ataaaagcaa tacacaaccc cagcggcaac
     8161 gaaataatcg ccaccagcgc cgagcgcaca tgccagagaa acagcgcaca gactaccgcc
     8221 accacaataa actcttccag caacttgccg ctgaggttgt cgatggcgcg gtcaatgagc
     8281 tggctgcgat cgtatgttgt aactatctcc acgccttccg gcagactact tttcagcgtt
     8341 tccagtttgt ccttcacggc ggcgatcact tctcgggcgt ttttgccgga tcgcaggatc
     8401 accaccccgc ccgccacttc gccttcgccg tttagttcgg caatgccccg gcgcatctcc
     8461 gggccaatct ggaccttcgc aacatcgcgc agataaacgg gcacgccatt ttcactggct
     8521 tttaaaacga tgtgattaaa gtcgtcgagc gtttgcagat agccgctggc gcgcaccata
     8581 tattccgctt ccgccagttc gatcgacgaa ccgcccgctt cctggtttga agcatccagc
     8641 gcgcttttta cttcggcgag actgatgcca tactgcgcca ggcgctgggg atcgataacc
     8701 acctgatact ctttcaccac accgcccacc gacgccactt ccgcaacgtc agggatggtt
     8761 ttcagctcat atttgagaaa ccagtcctgt aatgagcgta aatcggccag atcgtgctta
     8821 ccgctgcgat ccaccagtgc atattcatag atccagccaa cacccgtggc atctggtccc
     8881 agctcggcgc tgactcccgc aggcagctta ccctgtacct ggttgaggta ctccagcacc
     8941 cgcgaacgcg cccagtacgg atcggtgcca tcttcgaaaa tgacatacac ataagagtcg
     9001 ccaaactgtg agaaaccgcg cacagtcttc gcgccaggca ccgacaacat ggtggtggtt
     9061 agcggataag tcacctgatt ttcaacgatt tgtggtgcct gaccgggata gctggtttta
     9121 ataataacct gcacatcgga gagatccggc agcgcatcca ctggcgtatt aatgatggtc
     9181 caggtgcccc agatgctcag aaacaacgcg cccatcagca ccaaaaaacg gttcgccacc
     9241 gagcgacgaa taatccattc aatcattggt tattccctca atgcgcatgg gtagcacttt
     9301 cagagcgcat ccgctccagt gcgccagaaa tattggcttc ggaatcaatc aggaacaggc
     9361 cgctggaaac caccttttca ccttccgcca gaccagagcg taatgcggtg acgccttgcg
     9421 atgcctggaa aacagcaacg cgtttcggta caaagcgccc gtcggcatca acggtaatca
     9481 cccgctgttc gctgccggta tcaatcagcg cttgtgacgg aatgagcagc atcggttcgc
     9541 tggcggtgtt gagttgcaac caggcgttca tgcccggttt tagcgcctcg tcggcgttgt
     9601 cgacttccag acgcagctgc agcgtgcggg tcgcggcatc cacgccaggt agcagcgtcc
     9661 atttgcggat ggtgagtgtt ttatccggtc gcgccggaac ggtgagggta aactgcgagg
     9721 catctttcac cagccaggcg atagactccg ggatcgcagc agtgacccac accgggtcca
     9781 taccctgaat tttcgctacc acgttatctt tggcgatatt cattcccgcg cgcagatcaa
     9841 acgcggtgat cacgccatca atgggcgctt tgagcgtaaa gcgagtctgg attttttgcg
     9901 tggcgatcag gcggcgaata tccgcctccg gcattcccgc cagtcgcagc cgctcaagaa
     9961 tgccttcagt ctgggtcgcc gtaccgccgg tttcgcgcag cagtaaatac tcactctgcg
    10021 cttccaccca gtcaggaatg gtcaggtcga gaagcggtgt gcccttttgt actttatcgc
    10081 ccacggtaag cggatacacc ttgtcgataa acccggcagc gcgagcctgc acaatggcat
    10141 actgatactc gttgtaactg acattcgccg ggaaactctg ggcaaaagtc agcggcccgc
    10201 gcgtgacggt agccgttttc acccccagat tctgcgtctg agtcgggtca atgcgcacac
    10261 cagacgcaga actctcttca tcggcatatt tcggcaccag atccatatcc ataaacggcg
    10321 atttacctgg tttatcgaac cgcgtattgg ggtacatcgg gtcgtaccag aataagattt
    10381 tacgttctgc ggtcgacgtt ttttctgcgg gtggttcagc ctttgcaaac caggtaaaac
    10441 ctgccgcaga aataataccg cccgcgatca tgctgccgat aataagcgcg atttttttca
    10501 tctcattaaa cctgggttac tggctgactt taatatcctg taataaagaa aggttgccct
    10561 gctggacaaa attaaacgcc actttgtcgc cggttttaat ttcactcatt ttcgtctgcg
    10621 gggtgatggt aaagcgcatg gtcatctccg gccagttcac agcagcaatc ggatcgtgat
    10681 ggatggtgat ttttttgctt tccagatcaa taccctttac ctcgccagtg gcactaataa
    10741 cctgtggttg tgcttcgctc atggtttcat gatgatgttc gttagcctgg gcattaaagc
    10801 caataacggt aaacagactg aacattgcga cttgcagtgc ttttttcatt tattctctcc
    10861 tggagttaaa agttattgct gccaaccgcc accaagtgcg gtatataaag aaatttcgtt
    10921 aacctgacgg gcataattca ggtcaagtaa agtttgtcgg gttgcaaata acgaacgttc
    10981 ggcatccagc acttccagat aacttactgc gccgtgctga tataatgccc gcgcccgttg
    11041 caaagtaatt tgcagcgatg ccagataacg ctgctgggcg ctaatttgat cgttcaggct
    11101 ttgacgtaat gccagcgcat ctgccacttc tttaaaggcg ttctggattt tctgttcata
    11161 attcactacc gactgctgct ggcgaatttc ggcgatatcc agattggcct ggttgcgtcc
    11221 ggcattaaaa atggggatct caattttagg aataaaattc cacatcccgc tgctggcgtt
    11281 aaataatgac gacagatcgc tgctggcggt tgatattccg ctggtcaggc ttatagatgg
    11341 gaaaaatgcc gcgcgtgcgg ccccaatatt ggcattagcc gccattaacg cgtgttcagc
    11401 ttccataata tccgggcgct gcaataagat ttgcgacgac aagcccgccg gtaatttaac
    11461 gctttgcagg ctgtcgctgt ttactgtctg cgcttgcggc agcttgccgt agcttcccag
    11521 caacagttgc aatgcattat tcgcctgcgc cagttccccc tgacgtttag cgatgtcgct
    11581 gcgggtactt tctatcaccc cgcgagcctg ttccagcgcc agaacattac tgctacctgt
    11641 caacagttgt ttttcgacaa acgcatatga ctgctgataa ttacgcagcg tttcttcagc
    11701 tatttgcaat tgcgcatacg ccagttgctg attgaaatag ctttgcgcga cattagaaac
    11761 cagcagaata tgcaccgcgc gctgagcttc ctcagtggct aaataatttt ggcgctcggc
    11821 ttcgctcatg ttctttaagc gaccgaaaaa atcgaggtca aagctggcgt taaggccggt
    11881 cgaaaactcc cgtgtcgtgg cggtattccc tttaagatta ccgctccagc tgccgctgcc
    11941 ctcgccattg agctgtgggt agcggtcggc atcggtcaga cgatattgcg cccgcgcttc
    12001 ctgcactttc agcgtcgcca tgcgcaaatc ccggttattc accagcgcct cgctaatcag
    12061 cgtctttacc tgattatcaa caaaaaaggt gcgccagccc gcgttctggt agttatctgc
    12121 tgcgttaacc aagccgttct ggctgagtga gaactgctgc ggcacgggca ttgccggacg
    12181 ctgataatcc ggtgccagtg aacaaccggt tagcgcaagg gccacacaaa atggcagaag
    12241 tttacaagga gacataggct cataatttct ggtgatttta taccgccaac tttactcgcc
    12301 aggctctgat tttccggtga caggaaaatg acaaaattgt cattttgcca ataagcgatt
    12361 gccatctgat cccgctactc tagaattgcc cgggcaacat gcggaggaaa aatgaaactg
    12421 ttgattgtcg aagatgaaaa gaaaaccgga gaatacttga ccaaagggtt aaccgaagcc
    12481 ggttttgtgg tcgatttggc cgacaacggg ctgaatggct accatctggc gatgaccggt
    12541 gattatgatc tgataatcct cgatattatg ctgccggacg tgaacggctg ggatatcgtg
    12601 cgcatgttac gctccgccaa taaagggatg ccgattctgt tgcttaccgc gcttggcacc
    12661 attgaacatc gcgtcaaggg gctggagttg ggggcagatg actacctgat gaaaccattc
    12721 gcttttgctg aactgctggc gcgggtgcgc actctcctgc ggcgcggggc ggcggtaatt
    12781 atcgaaagtc agtttcaggt tgccgacctg atggtcgatc tcgtcagccg caaagtcacc
    12841 cgcagcggca cgcgcatcac tctgaccagt aaagagttta ctctgctgga gttttttctc
    12901 cgccatcagg gcgaagtgct gccccgctcg cttatcgcct cgcaggtgtg ggacatgaat
    12961 ttcgacagcg acactaacgc cattgatgtg gcggtgaagc ggctacgcgg caaaatcgac
    13021 aacgactttg agccgaagct gattcagacc gtgcgcggcg tgggctatat gcttgaggtg
    13081 ccggatggtc agtaagccat ttcagcgccc gttttcgctg gcaacccgtc tgaccttttt
    13141 tatcagcctg gccaccatcg cggcgttttt cgcctttgca tggatcatga tccactcagt
    13201 gaaagtgcat tttgtcgagc aggatattaa tgatttaaaa gaaattagcg ccacccttga
    13261 acgggtactt aatcatcctg acgagacgca agcccgacgc ttaatgacgc tggaagatat
    13321 cgtcagtggt tattccaacg tgttgatttc cctggcagat agccacggca aaacggtgta
    13381 taactccccc ggtgcgccgg atatccgcga gtttacgcgt gacgccatac ccgataaaga
    13441 cgctcagggt ggcgaggtgt atctcctttc cggcccgacg attatgatgc caggccacgg
    13501 tcacgggcat atggaacaca gcaactggcg gatgattaac ttaccggttg gcccgctggt
    13561 tgacggcaaa ccgatttata cgctctacat cgcgctttcg atcgattttc atcttcatta
    13621 cataaatgat ttgatgaata aacttattat gaccgcatcg gtaatcagca tcctgatcgt
    13681 ctttatcgtg ctgttggcgg tacataaagg tcacgcgccg atccgcagcg tcagccgtca
    13741 aatccagaat atcacctcga aagatctcga cgttcgcctt gacccgcaga ccgtgcccat
    13801 tgagctggaa cagctggtac tgtcgttcaa ccatatgatc gagcgtattg aggatgtctt
    13861 tacccgccag tccaatttct cagcggatat cgctcacgaa attcgcacgc cgattaccaa
    13921 tctcatcacg caaacggaaa tcgccctcag ccagtctcgc agccagaagg agctggaaga
    13981 tgtgctctac tctaatctcg aagagctgac gcgaatggcg aaaatggtca gcgatatgct
    14041 gtttctcgct caggccgata acaaccagct aatccccgaa aagaaaatgc tcaacctggc
    14101 ggatgaagtc ggcaaagtgt tcgatttttt cgaggcgtta gcggaagatc gcggcgtgga
    14161 gttgcgattt gttggcgacg aatgtcaggt tgcgggcgat ccgctgatgc tgcgtcgggc
    14221 gttaagcaac ctgctttcta atgccctgcg ttatacgcca cccagtgagg ctattgtagt
    14281 gcgctgccag acggtcaatc atcaggtgca agtttccgtc gaaaaccccg gtacgcccat
    14341 tgcgcccgag cacttaccgc gattgtttga ccgtttctat cgcgttgacc cttcccgcca
    14401 gcgaaaaggt gaaggtagcg gtattgggct ggcgatagtg aaatcgattg ttgtcgcgca
    14461 taaaggcacg gttgcggtaa cgtcagatgc gcgggggaca aggtttgtga ttatgctgcc
    14521 ggagagagag tgattattaa gttcaaattc agggcgtggt gcatcacctc ctggtattgt
    14581 tgatcaacaa attagagaaa aacttattaa acatatgaaa tatcgaaggc ttttattttt
    14641 caaagtttca cgagatgttt gtatatgaaa cttagttaaa tctgtgtttc ttccaattgt
    14701 agatcacaat gccctttcta aattttcat
//
LOCUS       DAZFQD010000132        14646 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.132, whole genome shotgun sequence.
ACCESSION   DAZFQD010000132 DAZFQD010000000
VERSION     DAZFQD010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14646
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.132"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..550)
                     /locus_tag="ACHKTW_004033"
     CDS             complement(<1..550)
                     /locus_tag="ACHKTW_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFO6464958.1"
                     /translation="MSKIDYQALRKAAQLATQGEWVAFISSGTGTYAVHTPGDKRCED
                     VIKWTGFDGQNNAENNARYIAAFNPEVVQALLDERERNQQYIKRRDQENEDIALTVGK
                     LRVELEAAKSKLNEQREYYEGVISDGCKRIAELEAREVQLPTRYDLRYGHPINADERQ
                     VMIPKENGSWLYLIDLEHALRVA"
     gene            complement(547..786)
                     /locus_tag="ACHKTW_004034"
     CDS             complement(547..786)
                     /locus_tag="ACHKTW_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000476205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6464959.1"
                     /translation="MFKQNEKAIAQIADYIPRACRGMQLQEAKARLEKKIALYIDDGC
                     DAAVLNAAFAPALNSHTRESFFSCIAAQIRKGGNQ"
     gene            complement(779..982)
                     /locus_tag="ACHKTW_004035"
     CDS             complement(779..982)
                     /locus_tag="ACHKTW_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019705292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6464960.1"
                     /translation="MTIVKTHTGTVITKDGPQVKKLHQTERMWVVGKNEFYHKETGRR
                     HFAENTRRRLLLDTIKPIEVKHV"
     gene            complement(979..1857)
                     /locus_tag="ACHKTW_004036"
     CDS             complement(979..1857)
                     /locus_tag="ACHKTW_004036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000335005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase family
                     protein"
                     /protein_id="HFO6464961.1"
                     /translation="MCITHVVSFSGGRTSAYLVHLMEEQRKAGNNVCYIFMDTGCEHP
                     LTYRFIREVVKFWGIPLTVLQVDINPELGQPNGYTEWEPKDIQTRMPVLKPFMDMVKK
                     YGTPYIGGAFCTDRLKLTPFTKYCDDHFGRGNYITWLGIRADEHRRLKPKPGVRYLAE
                     LSDFDKSDVIRWWRKQPFDLQIPEHLGNCVFCIKKSTQKLGIACKDEPGLMRVFNELV
                     TGKHVRDGHRRTGKDVMYRGHLTLDRIARMYADSDYRSLYQAMVLAKRFDTGSCSESC
                     EIWGDQLELKFEEVVA"
     gene            complement(1848..2384)
                     /gene="yfdR"
                     /locus_tag="ACHKTW_004037"
     CDS             complement(1848..2384)
                     /gene="yfdR"
                     /locus_tag="ACHKTW_004037"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HFO6464962.1"
                     /translation="MSFIKTFSGKHFYYDKINKDDIVINDIAVSLSNICRFAGHLSHF
                     YSVAQHAVLCSQLVPQEFAFEALMHDATEAYCQDIPAPLKRLLPDYKRMEEKIDAVIR
                     EKYGLPPVMSTPVKYADLIMLATERRDLGLDDGSFWPVLEGIPATEMFKVIPLAPGHA
                     YGMFMERFNELSELRKCA"
     gene            complement(2513..3337)
                     /gene="yfdQ"
                     /locus_tag="ACHKTW_004038"
     CDS             complement(2513..3337)
                     /gene="yfdQ"
                     /locus_tag="ACHKTW_004038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdQ family protein"
                     /protein_id="HFO6464963.1"
                     /translation="MSQNLDATAINQIHALISAQGVNEIISKIGADAVALPENFRIHD
                     LEKFNLNRFRFRGALSTASIDDFTRYSKDLADEGTRCFIDADNMRAVSVLNLGTIDEP
                     GHADNTATLKLKKTAQFSALLSVNGERNSQKSLAEWIEDWADYLVGFDANGDAIQATK
                     AAAAVRKITIEANQTADFEDNDFSGKRSLMESVEAKTKDIMPVAFEFKCVPFEGLKER
                     PFKLRLSIITGDRPVLVLRIIQLEAVQEEMANEFRDLLVEKFKDSKVETFIGTFTA"
     gene            complement(3403..3765)
                     /gene="yfdP"
                     /locus_tag="ACHKTW_004039"
     CDS             complement(3403..3765)
                     /gene="yfdP"
                     /locus_tag="ACHKTW_004039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YfdP"
                     /protein_id="HFO6464964.1"
                     /translation="MASERSTDVQAFIGELDGGVFETKIGAVLSEVASGVMNTKTKGK
                     VSLNLEIEPFDENRVKIKHKLSYVRPTNRGKISEEDTTETPMYVNRGGRLTILQEDQG
                     QLLTLAGEPDGKLRAAGH"
     gene            complement(4489..5181)
                     /locus_tag="ACHKTW_004040"
     CDS             complement(4489..5181)
                     /locus_tag="ACHKTW_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001358750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFO6464965.1"
                     /translation="MNIGNRVRQLRQAKNMKIADLAEAIGVDAANISRLETGKQKQFT
                     EQALSNIARSLGVDIADLFTSDLKSNTVCKNSISEDVAQVKDVFRIEMLDVSASAGNG
                     LIQGGDVIDVIHAIEYRTDNAVSMFGGRPANHIKVINVRGDSMCPTIEPGDLIFVDIS
                     INQFDGDGIYVFGFDDKIYVKRLQMIPDKLLVISDNQIYREWGITSENEHRFMVFGKV
                     LISQSQTLKRHN"
     gene            5279..5539
                     /locus_tag="ACHKTW_004041"
     CDS             5279..5539
                     /locus_tag="ACHKTW_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001191680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO6464966.1"
                     /translation="MQSPLRNVRKAHGFTLQHVAAGVQVNPATLSRIERLEQIPSIDL
                     AERLANFFKGEISEMQILYPARFQSSQNQNGFKPQEQEVSRG"
     gene            5532..6089
                     /gene="ymfL"
                     /locus_tag="ACHKTW_004042"
     CDS             5532..6089
                     /gene="ymfL"
                     /locus_tag="ACHKTW_004042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmfL"
                     /protein_id="HFO6464967.1"
                     /translation="MGKHHWKVEKQPEWYVKAVRKTIAALPGGYAEAADWLDVTENAL
                     FNRLRADGDQIFPLGWAMVLQRAAGTHYIADAVAQSAGGVFVSLPEIEAVENADINQR
                     LLEVIEQIGSYSRQIRSAIEDGVVEPHEKTAINDELYLSISKLQEHAALVYKIFCAPE
                     NSNARECAAPGVVASIASGCGETNA"
     gene            6235..6414
                     /locus_tag="ACHKTW_004043"
     CDS             6235..6414
                     /locus_tag="ACHKTW_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFO6464968.1"
                     /translation="MQELNRWFRDGRGLYVHVIRWEPETQRVIYLRKDYPHECFSPLW
                     KFRRDFVECEGPPAY"
     gene            6344..7246
                     /locus_tag="ACHKTW_004044"
     CDS             6344..7246
                     /locus_tag="ACHKTW_004044"
                     /inference="COORDINATES: protein motif:HMM:TIGR02681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFO6464969.1"
                     /translation="MSALVLCGNSGVILLSVKDHQHIDSAIPGRYTVQAPYKAGAGIG
                     VLKLSTAMYDAPASFLSSAFAHIQIMVGWAGAPKGAPVSVDAGYANPVQFTTSEIGVS
                     GGGSFSLSTEAAIMATNPALTQPEITIDNGQAVTTSLAVANSFSKRHDDVLKKIRTLD
                     CSPEFCARNFAETSISVNQPNGGTRKLPCYQITRDGFAFLAMGFTGKRAARFKEAYIN
                     AFNQMEKLLSKPSTLSDAADNASVLYSHLSVIHKVWLQQLYPMLAKAESPLAVSLYDR
                     INDAALLASLINLSLNPSEVRGRK"
     gene            7243..7467
                     /locus_tag="ACHKTW_004045"
     CDS             7243..7467
                     /locus_tag="ACHKTW_004045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000620694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6464970.1"
                     /translation="MIRNIFKRFTNQTFRCPRPGQWYTTPAGHVLRVSLVDRECQKVI
                     CEPLGRNYRISMPLIAFCSGKMFKRLGGVA"
     gene            7464..7763
                     /locus_tag="ACHKTW_004046"
     CDS             7464..7763
                     /locus_tag="ACHKTW_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000995578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO6464971.1"
                     /translation="MNCFQFVCGCAFDNPIQRLIMLRVLMSGSSDGEGERVIDHQVLA
                     DFCCCSKQAIFRETLALERAGYLHIRKIATLTIDAKARLQPARGYTILMLRKEVV"
     gene            8351..8743
                     /locus_tag="ACHKTW_004047"
                     /pseudo
     CDS             8351..8743
                     /locus_tag="ACHKTW_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000061490.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            8740..9234
                     /locus_tag="ACHKTW_004048"
     CDS             8740..9234
                     /locus_tag="ACHKTW_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFO6464972.1"
                     /translation="MIMSLMNDVQKFIEAHPGCTSGDIADAFAGYSRQCVLQSASKLR
                     QSGRVAHCCEGKTRRHFPHQAEISQEEKLQPVLETIPVRNFYVGTNDPRVILCLTRQA
                     EELESRGLYRRAATVWMAAFRESHSQPERNNFLARRERCLRKSRKRAVAGDEWYLSGN
                     YVGA"
     gene            9234..9887
                     /locus_tag="ACHKTW_004049"
     CDS             9234..9887
                     /locus_tag="ACHKTW_004049"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001378271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage N-6-adenine-methyltransferase"
                     /protein_id="HFO6464973.1"
                     /translation="MSNKYCQALVELRNKPAHELKEVGDQWRTPDNIFWGINTLFGPF
                     VLDLFTDGDNAKCAAYYTAEDNALAHDWSERLAELKGAAFGNPPYSRASQHEGQYITG
                     MRYIMKHASAMRDKGGRYVFLIKAATSEVWWPEDADHIAFIRGRIGFELPAWFIPKDE
                     KQVPTGAFFAGAIAVFDKTWKGAAISYIGRDELEACGEAFLAQVRQQAEKLVREMAA"
     gene            9884..10210
                     /locus_tag="ACHKTW_004050"
     CDS             9884..10210
                     /locus_tag="ACHKTW_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003878599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HFO6464974.1"
                     /translation="MTTLTQCQQQVLDMLISYQKERGFPPTNQEVATMLGYRSVNAAV
                     EHLRALEKKGVITIKRGVARGITLHTAVKDDDSEAVGIIRALLAGEANARLRAAHWLH
                     ERGLKV"
     gene            10207..10602
                     /locus_tag="ACHKTW_004051"
     CDS             10207..10602
                     /locus_tag="ACHKTW_004051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFO6464975.1"
                     /translation="MKLILPFPPSVNTYWRHPNKGAFAGKSLISAAGRKFQSAACAAI
                     VEQLRRLPKPTSAPASVEIVLFPPDNRIRDLDNYNKALFDALTHAGVWEDDSQVKRML
                     VEWGPVIPEGKVEITISKYEKASCKLATR"
     gene            10765..11580
                     /locus_tag="ACHKTW_004052"
     CDS             10765..11580
                     /locus_tag="ACHKTW_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005634 - nucleus [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HFO6464976.1"
                     /translation="MNQLLVIDGVSVRQYFESNYCLNDLQKAALLAAGENRSSRSLEA
                     HEFMRRPETKALVELLEEETTGDSRSIPVITIQGRNGGTYVCKELVYAYAMWISPAFS
                     LKVIRTFDALHNSSPEETTSDKIKSGVILLESAAKTLNLSNSSKLGAYQKLSKVAGLP
                     ELMPIYAIDAPADAPDGSSRPTLSLSALLKQYGIRLTANQAYHQMVKLGIVEQRERYS
                     RTAINNIKKFWSLTAKGCMFGKNITSPANPRETQPHFFESRFPELLKLLDTVH"
     gene            11596..12111
                     /locus_tag="ACHKTW_004053"
     CDS             11596..12111
                     /locus_tag="ACHKTW_004053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001223334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6464977.1"
                     /translation="MRITPPHLQPVLSRVKRFVERMPEGATLTQISQKVQAYSLLNKR
                     DKEILIGIIRDSGLLVVANDGRTTTLHHPKFGHQAVNSEIPVKTEEPVVIKKTVTPDE
                     LRRHAEELIRAAEEAEKKFNDRAEIKKQLDPLKLEILQAYGMASRKFDEFVDAMADMG
                     KAVQKLKQIVL"
     gene            12121..13110
                     /locus_tag="ACHKTW_004054"
     CDS             12121..13110
                     /locus_tag="ACHKTW_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFO6464978.1"
                     /translation="MRALLTPEIAPRMGIVLFRPGSELMPLFMQGRVLLEPEPERYSS
                     FASGAVPAASQPLADDPAVRAVFRNEAVIRRAGGVECLESWLLREKGCQWPHSDWHSE
                     NMTTMRHAPGAIRLCWHCDNLLRDQFTERLESMATDNCARWVLSVVRRDLGFDDSHVV
                     TMPELCWWLVRNDLADALPESAARKVLRLPKTVVPSVTRESDLVPSVPATSIIRDKAK
                     KVLALKVDPESPESFMLRPKRRRWVNEKYTRWVKTQPCACCGKPADDPHHLIGHGQGG
                     MGTKAHDLFVLPLCRKHHDELHADTVAFEEKYGSQLELIFRFIDRALAIGVLS"
     gene            13125..13520
                     /locus_tag="ACHKTW_004055"
     CDS             13125..13520
                     /locus_tag="ACHKTW_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFO6464979.1"
                     /translation="MMRDIQMVLERWGAWAASGNAGVDYSPIAAGFKGLLPSTAKPRP
                     ACSDDDGLIIENCLTRLKKKKPDEYSLLVAHYLLRISKRQIARTRKKSEKAIRIEMQI
                     AEGFIDGCLSMLGVRLEMDDWLPKKVKND"
     gene            13722..13919
                     /locus_tag="ACHKTW_004056"
     CDS             13722..13919
                     /locus_tag="ACHKTW_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HFO6464980.1"
                     /translation="MLKQQDMTETARVVFNELSVTYPATVGEIAQNTYLSRERCQLIL
                     TQLVMAGLADYQFGCYRRLPQ"
     gene            complement(14102..14233)
                     /locus_tag="ACHKTW_004057"
                     /pseudo
     CDS             complement(14102..14233)
                     /locus_tag="ACHKTW_004057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311526.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         3643 a         3508 c         3747 g         3748 t
ORIGIN      
        1 cagcgacgcg taatgcgtgt tctaggtcaa tcaggtaaag ccaactgcca ttttctttag
       61 gtatcatgac ttgtcgctca tctgcattta tcgggtgtcc atatcgaagg tcgtagcgag
      121 tcggtaattg aacttcccgc gcttccagtt cagcaatacg cttgcaccca tcagagataa
      181 ctccctcgta atactcgcgc tgctcgttga gttttgattt tgctgcttca agctcaacgc
      241 gcagcttccc aaccgtaagc gcaatatcct cgttctcctg gtcacggcgt ttgatgtatt
      301 gctggtttct ttcccgttca tccagcagcg cctgcactac ttcagggttg aaagctgcga
      361 tataacgagc gttgttctct gcgttgttct gtccatcaaa gccggtccat ttgataacgt
      421 cttcacatcg tttatcacca ggtgtatgca ccgcatatgt accagtacca gacgaaataa
      481 atgcgaccca ttcaccctgt gttgccagtt gcgccgcttt acgcagtgcc tgatagtcaa
      541 tcttgctcac tggttgcctc ctttgcggat ctgcgctgcg atgcacgaaa aaaaagactc
      601 ccgcgtatga ctgttaagag ctggcgcgaa cgctgcgtta agaacggcag catcacagcc
      661 gtcatcgata tagagcgcaa tttttttctc caggcgcgct ttggcttcct gcaactgcat
      721 accccggcac gcacgcggga tataatcagc aatttgagcg atagcttttt cgttctgttt
      781 aaacatgctt cacctcgata ggcttgatgg tgtctaacag cagtcggcgg cgcgtatttt
      841 ctgcaaagtg gcggcgtcca gtttctttgt ggtaaaactc gtttttgccg acgacccaca
      901 tccgttctgt ttggtgcaat ttttttacct gcggtccatc tttggtgatc acggtaccgg
      961 tatgggtttt tacgattgtc atgccactac ctcttcgaat ttcaactcca attggtcacc
     1021 ccagatttca catgattcgg aacacgaacc agtatcaaac cgcttggcca gcaccatcgc
     1081 ctgatacaaa cttctgtagt cgctgtcggc atacattctg gcaatcctgt caagcgtcag
     1141 gtgaccacgg tacataacgt ctttacctgt tctgcgatga ccatcccgga cgtgtttgcc
     1201 tgtaaccagt tcattaaaaa cccgcatcag accaggttcg tctttacatg cgatccccag
     1261 cttttgcgtt gactttttga tgcagaaaac acagttcccg aggtgctccg ggatttgcaa
     1321 atcaaaaggt tgtttacgcc accaccggat aacatccgac ttatcaaaat ctgacagctc
     1381 ggcaagatac cggacgcccg gtttcggttt cagcctacgg tgttcgtccg cacgaatacc
     1441 cagccatgtg atgtaattcc ctcgcccaaa atggtcatcg caatatttcg tgaaaggggt
     1501 gagttttagc ctgtcagtac agaatgcgcc gccgatgtat ggcgtaccgt actttttaac
     1561 catgtccata aacggtttaa gcaccggcat tcgcgtctga atatcctttg gttcccattc
     1621 cgtataacca tttggctgcc caagctctgg atttatatcg acctgcaaca cagttagcgg
     1681 tatgccccag aacttcacaa cctcccgaat aaagcggtat gtcagcggat gttcgcaacc
     1741 ggtatccata aagatgtagc agacgttatt gccagccttt ctttgttctt ccatcaggtg
     1801 aacaagatat gcagatgttc tcccgccaga aaaactaact acatgagtta tgcacatttg
     1861 cgtaattccg ataactcgtt gaagcgttcc ataaacatcc cgtaggcatg gcccggtgcc
     1921 agtggaatca ctttgaacat ctctgttgcc gggatacctt ccaatacagg ccagaaagag
     1981 ccatcatcaa gtccgagatc gcggcgttcg gttgccagca tgatgagatc ggcatatttc
     2041 actggcgtgc tcataacagg aggtaacccg tatttctcac ggattacggc gtctattttt
     2101 tcttccatcc gtttatagtc aggaagaagt cgtttcagtg gtgcggggat gtcctggcaa
     2161 tacgcttctg ttgcatcatg cattaacgct tcaaaagcaa attcctgcgg caccagctgg
     2221 ctgcaaagca ccgcatgctg ggcgacactg tagaagtgtg aaagatgtcc tgcaaagcga
     2281 cagatatttg aaagggaaac cgcgatatcg ttaatcacga tgtcgtcttt atttatcttg
     2341 tcataataaa aatgcttccc ggaaaaagtt ttaataaatg acatttcgtt ctccacttta
     2401 tatgcgctgc accgcgctga attcgggtaa aaggaatccc gcaccatccg gcgattattg
     2461 agttaattac gtttccataa atgcccccgc aggagcattt gcagtaatga aatcaggcgg
     2521 tgaaagtacc aataaaggtt tctactttgc tgtctttgaa tttctcaaca agcagatcac
     2581 gaaattcgtt agccatttct tcctgcactg cttccagctg aataatgcgc agaaccagta
     2641 caggacgatc gccagtgata atgctgaggc gtaatttaaa tggacgttct ttcagacctt
     2701 caaacggaac gcatttaaat tcaaatgcca ctggcataat atctttggtc ttcgcttcga
     2761 cagactccat cagggagcgt ttgccgctga agtcattgtc ttcaaaatca gcagtctggt
     2821 tcgcttcaat tgtgatttta cggactgccg cagccgcttt tgttgcctga atggcgtcac
     2881 cattagcatc aaagcccaca aggtagtcgg cccagtcttc aatccattct gccagtgact
     2941 tctgggagtt acgctcgccg ttaacagaca acagagcaga gaactgtgct gtctttttca
     3001 gtttgagagt ggcggtgtta tctgcgtgac ctggttcatc aatagtaccc aggttaagca
     3061 cactgacggc acgcatatta tcggcatcga taaagcagcg ggtgccttca tctgcaagat
     3121 ctttagaata acgggtaaag tcatcgatgc tggcagtgga aagcgcacca cggaaacgga
     3181 agcgatttaa attaaatttt tccagatcat gaatgcggaa attttcaggt aatgccactg
     3241 cgtcggcacc aatcttactg ataatttcat taacaccctg agcagaaata agggcatgga
     3301 tttgattaat tgcggttgcg tctaagttct gagacataat aagtcctcac tatattaaga
     3361 tattcagtga tgagataaat aatcagttaa ttaagaacga tattaatgac ctgctgcgcg
     3421 gagttttccg tcaggctcac cggcaagagt cagtaattgt ccctggtctt cctgcagaat
     3481 agtcaggcga ccaccgcgat tgacatacat cggcgtttcg gtggtgtctt cttcggaaat
     3541 tttcccgcgg ttagtcgggc gaacatatga gagtttgtgt ttgattttca cacggttctc
     3601 atcaaacggt tcgatttcca ggttgagcga gaccttacct ttggttttcg tgttcatcac
     3661 accggaagcg acttcactga gaactgcgcc gatttttgtt tcaaatacgc cgccgtccag
     3721 ctccccgata aatgcctgca catcagtact gcgttcgcta gccattttgt tgctcctcat
     3781 catatcgacc ctgcaaggtc ggttggtttc tccacaaaac agagaagaac acctgcggtg
     3841 gcagccgccc ggatggattg ggttatgagc ccgtcgtccg gtgatgctct tctctgtttt
     3901 gtaaaaagag cggtaccagc cggaagcaag tgtacaaact ggtaccgcca aagcagtggc
     3961 tgttgtggtg accggtgctg atctccggct tgcggttatt tcagactctc acgggcgttt
     4021 aattgccccg ccgaacagct cttttccgca atagctgcaa tgtctttcgc gcatcagcct
     4081 gcgcattcac cacaacgctg agagcactta gccagttacg gcaccacact ttgtcgcggt
     4141 tccataaatg ccctcatcgt tgcaccctgg tctcttccca ggtgtcaaac cgaaccgcca
     4201 cgctggttag gcgtcttatc agcatcatca ttgacttgca cattccggct acctggtttg
     4261 tttgcccgag caaggagtgg attgtcccct ttaacgtccc cagaccgcta acgacgcatg
     4321 tgccatacgc cgtgttacaa ccaaattttg ttagtacctt gtttgtatgt ctggaaagaa
     4381 agataaaatg aagttgcgca ttatgcaagt gtttttattg cgagatatgc aatttggtga
     4441 gtaatgaaaa gccaccttct ggtggctaat tgatattgag gtagggggtt aattgtgtcg
     4501 cttaagggtt tgtgactgac tgattaagac ctttccaaag accataaacc gatgttcgtt
     4561 ttcgctggta attccccatt cgcggtaaat ctgattatca gaaattacca gcagtttatc
     4621 aggtatcatt tgcagtcgtt tgacgtaaat tttatcatca aaaccaaata catatatacc
     4681 atccccatca aactgattga tactgatatc aacgaagatg agatctcctg gctcaatggt
     4741 tggacacata ctgtccccac gaacgttgat aactttaatg tgatttgctg gtcgtccacc
     4801 aaacatcgat acagcattat cagttctgta ttcaatggca tgaatcacat caatgacatc
     4861 accgccctgg ataaggccat ttcccgcact ggcactgaca tccagcattt caatacggaa
     4921 tacatccttc acctgcgcaa catcctcact aatactgttt ttacatacag tattactttt
     4981 gaggtctgag gtaaagagat cagcaatatc aacacctaag ctcctggcaa tattactcag
     5041 ggcttgttca gtgaattgtt tctgcttacc tgtttcgagg cgcgagatat tcgccgcatc
     5101 cactcctatt gcttcagcga gatcggcgat tttcatgttc ttcgcctggc gaagttgtct
     5161 gactcgattt cctatgttca tgcgtttatt acatttcttt attgcgcgtt aagcaaatca
     5221 acttgcgcaa aatatttgcg tgaaataata tgctcatcac gcaatatgtg gaggttatat
     5281 gcaatcacca ttacggaatg tgcgtaaggc gcacggattt actttgcagc atgttgctgc
     5341 gggcgttcag gtcaatccag cgacgctgag tcgtattgaa agactggaac aaattccatc
     5401 tatcgatctt gcagaacgtc tggccaattt ttttaagggt gaaatcagcg aaatgcagat
     5461 tctttatccg gcacgttttc aatctagcca aaaccagaat gggtttaaac cacaggaaca
     5521 ggaggtaagc cgtgggtaag catcactgga aagtggaaaa acaacctgag tggtacgtga
     5581 aagctgtcag aaaaactatc gcggcattgc ctgggggtta cgctgaagct gctgactggc
     5641 tggatgtaac agagaacgct ttattcaacc gccttcgtgc agatggcgat cagattttcc
     5701 cgctgggatg ggcaatggtt ttacagcgtg cggctggcac tcactacatt gcggatgctg
     5761 tcgcacagtc tgctggtggg gtgttcgtat cgcttcctga aattgaggca gtagagaacg
     5821 ccgatataaa ccagcgcctg ctggaagtca ttgaacagat cggcagttat tccagacaga
     5881 ttcgttcagc aatcgaagac ggtgtagtgg aaccgcatga gaagacagca attaacgacg
     5941 aactgtatct ttcaatttcg aagctccagg agcatgcagc actggtctac aaaatcttct
     6001 gcgctccaga aaatagtaac gcccgcgagt gtgcagctcc gggcgtcgtg gcgtcgattg
     6061 cttctggttg tggagaaact aacgcatgaa tagtttaacg gcaaataacc gtttgtcgca
     6121 acagctggtg gtcagcgtcg ctgaacacct gttgttacgg catgaatgca gattaccaaa
     6181 tcacctggct gtaagtaacc acagagaact ttacctgact gtggggggcg agttgtgcag
     6241 gaacttaacc gctggtttcg tgacggaaga gggctttatg tccatgttat tcgttgggag
     6301 ccagaaacac agcgcgttat ctatcttcgc aaagactacc cgcatgagtg ctttagtcct
     6361 ttgtggaaat tcaggcgtga ttttgttgag tgtgaaggac caccagcata ttgattctgc
     6421 cattccggga cgttacactg ttcaggcacc ttataaagcg ggtgccggga ttggcgtcct
     6481 gaaattgtca acggcgatgt atgacgcgcc agcgtctttt ttatcgtccg catttgctca
     6541 catccagatt atggtgggct gggcgggggc accgaaaggt gcgccggtct ccgttgacgc
     6601 cggttacgcc aaccccgtcc agttcaccac cagtgaaatt ggcgtttccg gtggtggaag
     6661 tttttcactg tcaacggagg ctgccatcat ggctacgaac ccagccctca ctcaacctga
     6721 aatcaccatt gacaacggcc aggccgttac cacttctttg gctgttgcca actccttctc
     6781 caagcgtcac gacgatgtgc tgaaaaagat ccgcactctg gattgttccc cagagttttg
     6841 tgcccgcaat tttgcggaga catcgatttc ggtaaatcaa ccgaacggtg gtacacgcaa
     6901 gctcccttgc tatcaaatca cccgcgacgg ttttgcgttt cttgctatgg gtttcacggg
     6961 taaacgtgct gcccggttca aagaggcata catcaatgcc tttaaccaga tggaaaaact
     7021 gctttcaaag ccatccacgc tgagcgatgc cgcagataac gccagcgtgc tttactccca
     7081 cctgtcggta atccacaagg tctggctgca gcagctttat cccatgttgg caaaagccga
     7141 atccccgctg gctgtaagtc tgtatgaccg catcaacgac gcggcgctac tggccagtct
     7201 cataaatttg tcgctgaacc cttcagaggt aagggggcgc aaatgatccg gaatattttc
     7261 aaacggttta ccaatcagac tttccgttgt cctcgtcctg gtcagtggta caccacgcct
     7321 gcagggcatg ttctacgtgt cagcctggtt gaccgtgaat gtcagaaggt gatttgtgaa
     7381 ccgctgggcc gtaattaccg catcagtatg ccgcttatag ccttttgctc cggaaaaatg
     7441 tttaagcgtc tgggaggtgt ggcgtgaact gttttcagtt tgtgtgcgga tgtgctttcg
     7501 ataacccgat tcagcgcctg attatgttgc gcgttttgat gtcgggttct tcagacggtg
     7561 aaggcgagag agttattgat catcaggtgc ttgctgattt ctgctgttgt tctaagcaag
     7621 cgatattcag ggaaaccctg gcactggaaa gagctggtta tcttcatatc cgaaaaattg
     7681 caacgcttac tattgatgca aaagccagac tacaacctgc gcgtggctac acaattctca
     7741 tgctgcggaa ggaggttgta tgagccgtta cgcccccaca ccggaagtta tggctattgg
     7801 tcaaattaat atttccggca atgttacacc tgcgacctgg tggaaatata ttcgactacc
     7861 cagtgggcgt ccggatgcga cggctatcgc tctgctttca gagatcgttt actggtaccg
     7921 cccgacagag gtcagggatg agcacaccgg agcgttgctg ggatatcaca agcgttttca
     7981 gggcgacaaa ctgcaaagaa gctaccaggc gtttgctgag cagtttggtt tcgggaaaag
     8041 ggaaaccgca gatgcgctga agcgtctgcg cgatgcaggg tttattactc tggatttacg
     8101 cacggtggaa atgctcgatg gggtgaaatg cagcaatatt ttgtttgtcg ggatcaaccc
     8161 acaggcaatt gcggccatca ccacaccttc ttctgtttcg ccagaaagta acagcaataa
     8221 tgcaatcagc gatacagcta ttacgttaaa acggaacacc ccccgacgtc ataacggaac
     8281 aggggatacg ccgaatgttg atacaaatac agagattact acagagatta caacggagac
     8341 taaaaacact attgatgcat ccgctgacgc gtctgcgcca gcgcgttctg cccgacagga
     8401 atattcaccg gaatttgaac aggcctggca ggaatatccc aaacgtgctg gtggcaattc
     8461 aaaatcagca gccttcaaag cctggaaagc ccgtttgaac gagggggtaa accccgaaac
     8521 catgctggaa ggtgtgaaac gctacgcggg ctgggtatct gcgatgggca atagcggcac
     8581 acaatttgtg aaacaggctg tcacgttctt tggtccggat cgtcatttcg aagaatcctg
     8641 ggaagttcct gcggtatctg cagccagacg cgaggacccg tacttcaaag ccagttacga
     8701 caacgtggac tacagccaga tcccgacagg attcaggggg tgatcatgag tctgatgaac
     8761 gatgtacaga aattcattga agcccatccg gggtgtactt caggtgacat tgcggatgct
     8821 tttgctggtt actcacgaca gtgcgttctg cagtcagcaa gcaagttacg tcagagtggc
     8881 cgtgtggctc actgttgtga agggaaaaca cgtagacatt tcccacacca ggctgagata
     8941 tcgcaggagg agaaactgca acctgttctt gaaaccatac ctgtgcgcaa tttctatgtc
     9001 ggcactaacg atccccgggt gattttgtgc ctgacccgcc aggcggaaga actggagtcc
     9061 aggggcttat accgtcgtgc tgcaaccgtg tggatggcgg cattccgtga aagccactcc
     9121 cagccagaac gaaacaattt tctggcgcgt cgtgagcggt gcttacggaa aagcagaaag
     9181 cgcgctgtag cgggtgatga gtggtatctg tcagggaatt acgtgggggc ttaatgagta
     9241 ataaatattg ccaggcgctg gtggaactac ggaacaaacc agcccatgaa ctgaaggaag
     9301 tgggcgatca gtggcgcacg ccggacaaca ttttctgggg aattaacacc ctgtttggcc
     9361 cgtttgttct ggatctgttt actgacggtg ataacgccaa atgtgccgcg tattacacgg
     9421 cggaagacaa cgcgctggca catgactggt cagaacgtct tgcggagctt aaaggtgctg
     9481 cctttggtaa tcccccgtac agccgcgcca gtcagcatga gggacaatac atcaccggca
     9541 tgcgttacat catgaagcat gccagtgcca tgcgtgataa aggcgggcgc tatgttttcc
     9601 tgatcaaagc ggccaccagc gaagtgtggt ggccggaaga tgcagatcat attgctttta
     9661 ttcgcgggcg tattggtttt gaactgccag cctggtttat cccgaaagac gaaaagcagg
     9721 tgccaacagg tgctttcttc gctggtgcta ttgctgtttt cgacaagacc tggaagggag
     9781 cggcaatcag ctacatcggg cgcgatgaac ttgaggcatg tggtgaggcc tttctggcgc
     9841 aggttcgcca gcaggcggaa aaactggtca gggagatggc ggcatgacga cattaactca
     9901 atgccagcag caggtgctgg atatgctgat ttcttatcag aaagaacgtg gcttcccgcc
     9961 aaccaatcag gaggtggcaa ccatgctggg ataccgttcg gtgaatgcag cggtggagca
    10021 tcttcgagca ctggagaaaa aaggtgtcat cacgataaag cgtggcgtgg cccggggtat
    10081 cactcttcat accgcggtga aggacgacga cagcgaggcg gtcgggatta tccgcgccct
    10141 gcttgccggt gaggcaaacg ccaggctgcg tgcagcccac tggttacatg agaggggcct
    10201 gaaagtatga agctaatact gccttttccg cccagcgtga acacgtactg gcgacaccct
    10261 aacaaagggg cgtttgctgg taagagcctg ataagcgcgg cggggcgaaa attccagagc
    10321 gcggcgtgtg cagcaatagt tgagcagtta cgtcgtctgc cgaaaccaac gtcggcacct
    10381 gcttcagtgg agatcgtgtt gtttcctccg gataaccgga tccgcgatct ggacaactat
    10441 aacaaggcgc tgtttgacgc cctgacccac gcgggtgtgt gggaagacga cagccaggtg
    10501 aaaagaatgc tggtggagtg gggaccggtt atcccggaag ggaaggtcga gatcactatc
    10561 agtaagtacg aaaaagcgag ttgcaaatta gcaactcggt aacggaattg agcaacaccc
    10621 taaatttggg tattacctcg ttaaagatac tgtatttatg aacagtgtat ccttgataac
    10681 tattaaaaat cgcagtaagt tcatcctgca tcaacgaaaa gggagtgcag tcccgctcgt
    10741 ggataaaaat ttgtggagaa accaatgaat cagttgcttg taattgatgg cgtttctgtg
    10801 cgccagtact tcgaatctaa ctactgtctt aacgaccttc agaaagctgc tcttcttgcc
    10861 gctggtgaga atcgctcctc ccgttcgctg gaagctcacg agtttatgcg tcgtcctgaa
    10921 acgaaggctc ttgtggaatt attggaagaa gaaactacgg gagattcccg tagtattcct
    10981 gtcatcacca ttcaggggcg caatggtggg acgtatgtct gtaaagagct ggtctatgca
    11041 tatgcaatgt ggatcagccc ggcattcagc ttaaaagtga tacgtacttt tgatgcgctt
    11101 cataattcat caccagaaga aaccacatcc gacaaaatta aatccggggt cattctgctt
    11161 gaatcagcag caaagactct aaatctgtca aactcctcga aacttggtgc ataccagaaa
    11221 ttatcaaagg tagctggtct tcctgaactt atgccgatct atgccattga tgcacctgct
    11281 gatgcgccag atggttcaag ccgccctacg ctgtcgctga gtgcactgct gaagcagtat
    11341 ggtatccgcc tgacggctaa tcaggcatat caccagatgg tgaaactggg gatcgtcgag
    11401 cagcgcgaac gatacagccg taccgcgatt aacaacatca aaaaattctg gtcgctgaca
    11461 gcgaaaggct gcatgttcgg caagaacatc accagtcctg caaatccgcg cgagacgcag
    11521 ccgcatttct tcgaatcccg attccctgag ctgttaaagc tgctcgatac cgttcactga
    11581 tgggaggcgt ggagcatgag aattacacca ccccatctgc agccagtttt atccagggtt
    11641 aaacgttttg ttgaacgaat gccggaaggc gcaacactga cccagatatc acagaaagtg
    11701 caggcgtaca gtctactgaa taaaagggat aaggagatac tcattggcat tatccgcgac
    11761 agtggacttc tggtcgttgc gaatgatgga agaactacaa cgttacatca tcctaaattt
    11821 ggacatcagg cagtaaatag tgaaatacca gtaaaaacag aggaacccgt tgtgattaaa
    11881 aagaccgtta ctccggatga attacgcagg catgctgagg agctgatcag ggctgcggaa
    11941 gaagcagaga agaaatttaa tgatcgtgcg gaaattaaaa agcaactgga tcctctgaaa
    12001 ctggaaatcc tccaggcgta tggaatggca agtcgtaaat ttgatgagtt tgttgatgct
    12061 atggcggata tggggaaagc ggtacagaaa ctgaaacaga ttgtgctgtg aggttctacg
    12121 ttgagagcac tactgacccc tgaaattgcc ccgcgtatgg ggatcgtatt gttcaggcca
    12181 ggttcagagc tgatgcccct gtttatgcag gggcgtgtcc tgctggagcc tgagccggag
    12241 cgttattcat ctttcgccag tggtgccgtt ccggcggcat cacaaccgct ggcggatgat
    12301 cctgccgttc gggccgtgtt ccgcaatgag gcggtgatcc gtcgtgctgg tggcgtggaa
    12361 tgccttgaga gctggttact tcgtgaaaag ggctgtcagt ggcctcattc cgactggcac
    12421 agcgagaaca tgaccacaat gcggcacgcg ccaggcgcaa tccgtctgtg ctggcactgt
    12481 gacaatctcc tccgtgacca gttcacggaa cggctggaat caatggcaac ggataactgt
    12541 gcccgctggg tgttgtctgt tgtgcgccgt gatcttggtt ttgatgacag tcacgttgtg
    12601 acaatgccgg aactgtgctg gtggctggtt cgtaatgacc tggcggatgc cttaccggaa
    12661 agtgcagccc gtaaggtact gagattaccg aagacggttg tgccgtctgt cacccgggaa
    12721 agtgaccttg tgccttcggt tcccgccacc agtattatcc gggataaagc gaaaaaggtg
    12781 ctggcgctga aagtggatcc ggagtcgccg gagtctttta tgttacgccc aaaacgtcgc
    12841 cgctgggtta atgaaaagta cacgcgctgg gttaagacgc agccgtgcgc atgttgtgga
    12901 aaacctgctg atgatcccca ccacctgata ggtcacggtc agggcggaat gggtacaaaa
    12961 gcgcatgacc tctttgtgtt gcctttgtgc agaaagcatc acgacgagct gcatgcggat
    13021 accgtggcat ttgaagagaa gtatggctcc cagctggagc tgatatttcg ttttatcgat
    13081 cgcgcgctgg cgattggtgt gctgtcctga ttttgtggag aaagttgatg cgtgatattc
    13141 agatggttct cgaacgctgg ggggcatggg cggcaagtgg taacgccggg gtggactatt
    13201 ctccgatagc tgctggattt aaaggccttt taccatccac cgctaaacct cgcccggcct
    13261 gcagcgatga tgacggcctt atcatcgaaa actgccttac gcgcctgaag aagaaaaaac
    13321 cggacgagta ttcgctgctg gtagctcatt atctgctgcg catatcaaaa aggcagattg
    13381 ccagaacaag aaagaagagc gaaaaggcaa tacgaattga gatgcagatt gctgaaggat
    13441 ttattgacgg atgtctgtcg atgctgggtg taaggctgga gatggacgac tggctgccca
    13501 aaaaagtaaa aaatgattag cgcggtccgc aaaaagtatg tcagtatgtt aagagtggtt
    13561 actacgccac acaacttaaa cccgccgcct ggcgggtttt ttatgactga aatcgcatca
    13621 gtacagtaaa cgtgctggtg gtgaatacct gtctttcagc ttgctggctt tttagacaag
    13681 agttattggt atgtcacgtt aaccggaaaa gggaaaaaga catgctaaaa cagcaggata
    13741 tgacagaaac cgccagagtg gtgtttaatg aattaagcgt cacctacccg gcgacagtcg
    13801 gggagattgc gcagaatact tacctttcac gcgaacgctg ccagttaata ctgacccagc
    13861 tggttatggc gggtctggca gactatcagt tcggttgtta cagacgcctt ccgcagtgaa
    13921 ggctttttta tttgtggtaa atgggcggct ggtgggtgtg gtggttgttg ctttcccgtt
    13981 gctgaaaaag aaagcatcag gcgattagca gggtatcagt tacccgttga aatttttaaa
    14041 tacctcacaa ttcaggcggt tgactgttgt ctggtttgcg gggagtttgt taaaagaaac
    14101 tggcatggtg aatccccctg tgcggagggg caatcagcaa ctggtgtttt gtcaccgacc
    14161 cttatccttt ctgtgcgggt tcaggtgctg atactgaact caccgggagg cacccggcac
    14221 catgcaatgg cacatagcgc cactctccag cccctctccg gaggggcttt tctgtgccgg
    14281 atacatcaca gtttctggaa ccttgggtac tacagtatca gtcagggtgc tatattttca
    14341 gatgtgatga aagcctgtca gcaggcaggg cgtatcggaa atgacccagt agagaaaacg
    14401 ttgactcaga taccggtgct gagttaccgg gaaaccggca tcacatgacc gctatccttc
    14461 caggcccatc cgctccggtg ggccttttta ctgcagaaaa caggttcccc gttaaatgct
    14521 atgttgctca caattcagta agttgacagt tgcctgtcag actgggcatt tgttaaaaaa
    14581 atttcgcatg gtgaatcccc ctgagcggag gggcgactgg tgacggtata atctctgatt
    14641 atcaaa
//
LOCUS       DAZFQD010000133        14621 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.133, whole genome shotgun sequence.
ACCESSION   DAZFQD010000133 DAZFQD010000000
VERSION     DAZFQD010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14621
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.133"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            127..2334
                     /gene="traD"
                     /locus_tag="ACHKTW_004058"
     CDS             127..2334
                     /gene="traD"
                     /locus_tag="ACHKTW_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001577029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system coupling
                     protein TraD"
                     /protein_id="HFO6464981.1"
                     /translation="MSFNAKDMTQGGQIASMRIRMFSQIANIMLYCLFIFFWILVGLV
                     LWIKISWQTFVNGCIYWWCTTLEGMRDLIKSQPVYEIQYYGKTFRMNAAQVLHDKYMI
                     WCSEQLWSAFVLAAVVALVICLITFFVVSWILGRQGKQQSENEATGGRQLTDNPKDVA
                     RMLKKDGKDSDIRIGDLPIIRDSEIQNFCLHGTVGAGKSEVIRRLANYARQRGDMVVI
                     YDRSGEFVKSYYDPSIDKILNPLDARCAAWDLWKECLTQPDFDNTANTLIPMGTKEDP
                     FWQGSGRTIFAEAAYLMRNDPNRSYSKLVDTLLSIKIEKLRTFLRNSPAANLVEEKIE
                     KTAISIRAVLTNYVKAIRYLQGIEHNGESFTIRDWMRGVREDQKNGWLFISSNADTHA
                     SLKPVISMWLSIAIRGLLAMGENRNRRVWFFCDELPTLHKLPDLVEILPEARKFGGCY
                     VFGIQSYAQLEDIYGEKAAATLFDVMNTRAFFRSPSHKIAEFAAGEIGEKEHLKASEQ
                     YSYGADPVRDGVSTGKDMERQTLVSYSDIQSLPDLTCYVTLPGPYPAVKLSLKYQARP
                     KVAPEFIPRDINPEMENRLSAVLAAREAEGRQMASLFEPDVPEVVSGEDVTQAEQPQQ
                     PQQPQQPQQPQQPQQPQQPMSPAINDKKSDSGVNVPAGGIEQELKMKPEEEMEQQLPP
                     GISESGEVVDMAAYEAWQQENHPDIQQQMQRREEVNINVHRERGEDVEPGDDF"
     gene            2334..7604
                     /gene="traI"
                     /locus_tag="ACHKTW_004059"
     CDS             2334..7604
                     /gene="traI"
                     /locus_tag="ACHKTW_004059"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
                     /protein_id="HFO6464982.1"
                     /translation="MMSIAQVRSAGSAGNYYTDKDNYYVLGSMGERWAGRGAEQLGLQ
                     GSVDKDVFTRLLEGRLPDGADLSRMQDGSNRHRPGYDLTFSAPKSVSMMAMLGGDKRL
                     IDAHNQAVDFAVRQVEALASTRVMTDGQSETVLTGNLVMALFNHDTSRDQEPQLHTHA
                     VVANVTQHNGEWKTLSSDKVGKTGFIENVYANQIAFGRLYREKLKEQVEALGYETEVV
                     GKHGMWEMPGVPVEAFSGRSQAIREAVGEDASLKSRDVAALDTRKSKQHVDPEVRMAE
                     WMQTLKETGFDIRAYRDAADQRAETRTQAPGAVSQEGPDVQQAVTQAIAGLSERKVQF
                     TYTDVLARTVGILPPENGVIERARAGIDEAISREQLIPLDREKGLFTSGIHVLDELSV
                     RALSRDIMKQNRVTVHPEKSVPRTAGYSDAVSVLAQDRPSLAIVSGQGGAAGQRERVA
                     ELVMMAREQGREVQIIAADRRSQMNLKQDERLSGELITGRRQLLEGMAFTPGSTVIVD
                     QGEKLSLKETLTLLDGAARHNVQVLITDSGQRTGTGSALMAMKDAGVNTYRWQGGEQR
                     PATIISEPDRNVRYARLAGDFAVSVKAGEESVAQVSGVREQAILTQAIRSELKTQGVL
                     GHPEVTMTALSPVWLDSRSRYLRDMYRPGMVMEQWNPETRSHDRYVIDRVTAQSHSLT
                     LRDAQGETQVVRISSLDSSWSLFRPEKMPVADGERLRVTGKIPGLRVSGGDRLQVASV
                     SEDAMTVVVPGRAEPATLPVADSPFTALKLENGWVETPGHSVSDSAKVFASVTQMAMD
                     NATLNGLARSGRDVRLYSSLDETRTAEKLARHPSFTVVSEQIKARAGETSLETAISHQ
                     KSALHTPAQQAIHLALPVVESKNLAFSHVDLLTEAKSFAAEGTSFTELGREIDAQIKR
                     GDLLHVDVAKGYGTDLLVSRASYEAEKSILRHILEGKEAVTPLMERVPGELMEKLTSG
                     QRAATRMILETSDRFTVVQGYAGVGKTTQFRAVMSAVNMLPESERPRVVGLGPTHRAV
                     GEMRSAGVDAQTLASFLHDTQLQQRSGETPDFSNTLFLLDESSMVGNTDMARAYALIA
                     AGGGRAVASGDTDQLQAIAPGQPFRLQQTRSAADVAIMKEIVRQTPELREAVYSLINR
                     DVERALSGLESVKPSQVPRLEGAWAPEHSVTEFSHSQEAKLAEAQQKAMLKGEAFPDV
                     PMTLYEAIVRDYTGRTPEAREQTLIVTHLNEDRRVLNSMIHDALEKAGELGKEQVMVP
                     VLNTANIRDGELRRLSTWETHRDALALVDNVYHRIAGISKDDGLITLEDAEGNTRLIS
                     PREAVAEGVTLYTPDTIRVGTGDRMRFTKSDRERGYVANSVWTVTAVSGDSVTLSDGQ
                     QTREIRPGQEQAEQHIDLAYAITAHGAQGASETFAIALEGTEGNRKQMAGFESAYVAL
                     SRMKQHVQVYTDNRQGWTDAINNAVQKGTAHDVFEPKPDREVMNAERLFSTARELRDV
                     AAGRAVLRQAGLAGGDSPARFIAPGRKYPQPYVALPAFDRNGKSAGIWLNPLTTDDGN
                     GLRGFSGEGRVKGSGDAQFVALQGSRNGESLLADNMQDGVRIARDNPDSGVVVRIAGE
                     GRPWNPGTITGGRVWGDIPDNSVQPGAGNGEPVTAEVLAQRQAEEAIRRETERRADEI
                     VRKMAENKPDLPDGKTEQAVRDIAGLERDRSAISEREAALPESVLREPQRVREAVREV
                     ARENLLQERLQQMERDMVRDLQKEKTLGGD"
     gene            7624..8370
                     /gene="traX"
                     /locus_tag="ACHKTW_004060"
     CDS             7624..8370
                     /gene="traX"
                     /locus_tag="ACHKTW_004060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
                     /protein_id="HFO6464983.1"
                     /translation="MTTDNTNTTRNDSLAARTDTWLQSLLVWSPGQRDIIKTVALVLM
                     VLDHANRIRHLDQSWMFLVGRGAFPLFALVWGLNLSRHAHIRQPAINRLWGWAMIAQF
                     AYYLAGFPWYEGNILFAFAVAAQVLTWCETRSGWRTAAAILLMALWGPLSGTSYGIAG
                     LLMLAVSHRLYRAEDRTERLVLVACLLAVIPALNLASSDAAAVAGLVMTVLTVGLVSR
                     VGKSLPRFWPGDFFPTFYGCHLAVLGILAL"
     gene            8429..9289
                     /locus_tag="ACHKTW_004061"
     CDS             8429..9289
                     /locus_tag="ACHKTW_004061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO6464984.1"
                     /translation="MKITDHKLSEGIALTFRVPEGNIKHPLIILCHGFCGIRNVLLPC
                     FANAFTEAGFATITFDYRGFGESDGERGRLVPAMQTEDIISVINWAEKQECIDNQRIG
                     LWGTSLGGGHVFSAAAQDQRVKCIVSQLAFADGEVLVTGEMNESERASFLSTLNKMAE
                     KKKNTGKEMFVGVTRVLSDDESKVFFEKIKARHPEMDIKIPFLTVMETLQYKPAESAA
                     KVQCPVLVVIAGQDSVNPPEQGRALYDAVASGTKELYEEADACHYDIYEGAFFERVVA
                     VQTQWFKQYL"
     gene            9392..9949
                     /locus_tag="ACHKTW_004062"
     CDS             9392..9949
                     /locus_tag="ACHKTW_004062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000139380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HFO6464985.1"
                     /translation="MAEQKRPVLTLKRKTEGETPVRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLTLPSLDEAVNTLKPWWPGLFDGNTPRLLACGIRE
                     VLLDEVSQRNIPLSHKKLRRALKAITRSESYLGAMKAGACRYDTEGYVTEHITQEEEQ
                     YAQARLEKVRRQNRIKDELRAILAE"
     gene            10104..10307
                     /locus_tag="ACHKTW_004063"
     CDS             10104..10307
                     /locus_tag="ACHKTW_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6464986.1"
                     /translation="MAVSSGLSEHSSIYRYQREGRHAVRQEIRGFFREAAEVWQNAAR
                     LAPCEAWRDFALKRASACRCKCR"
     gene            11050..11511
                     /locus_tag="ACHKTW_004064"
     CDS             11050..11511
                     /locus_tag="ACHKTW_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HFO6464987.1"
                     /translation="MKKYIPVVLFVFSCQVFSADIHGRVVRVLDGDTIDVMLSQHPVR
                     VRLVNIDAPEKKQEYGRWSEKIMKSLVAGKTVTVTYFQRDHYGRILGQVYAPDGMNVN
                     QFMVRAGDAWVYEQYNTDPVLPVLQGEVRQQKRGLWADSAPVPPWVWRSRK"
     gene            11614..11997
                     /locus_tag="ACHKTW_004065"
     CDS             11614..11997
                     /locus_tag="ACHKTW_004065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6464988.1"
                     /translation="MNYQAVSELITSSNHNVLIVGESASEVDSFLNKLNITDYKYYDF
                     SQIYSCSDRTLNDYAVIFIRNALNASEHIIIFNCTGWSDLNNESAVMQFARVARKSGK
                     QLIVAVREQDMKKMEAEFGRIIKIH"
     gene            12277..12426
                     /locus_tag="ACHKTW_004066"
     CDS             12277..12426
                     /locus_tag="ACHKTW_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO6464989.1"
                     /translation="MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLA
                     YEARK"
     gene            12710..12958
                     /locus_tag="ACHKTW_004067"
     CDS             12710..12958
                     /locus_tag="ACHKTW_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HFO6464990.1"
                     /translation="MSQTENAVTSSSGTKRAYRKGNPLTLAERQQASLARKRATHKEL
                     RVFIPAALKAQLQEMCEAEGVTQAEMIAELIKQKSAFS"
     gene            complement(13031..13174)
                     /locus_tag="ACHKTW_004068"
     CDS             complement(13031..13174)
                     /locus_tag="ACHKTW_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005023896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepA"
                     /protein_id="HFO6464991.1"
                     /translation="MVGYAVLWGPGNLLVAKHRKIIGVFAFLAPVHPHQNQFPATLWR
                     GQP"
     gene            13200..13274
                     /gene="tap"
                     /locus_tag="ACHKTW_004069"
     CDS             13200..13274
                     /gene="tap"
                     /locus_tag="ACHKTW_004069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HFO6464992.1"
                     /translation="MPGKVQDFFLCSLLLRIVSAGWCD"
     gene            13267..14124
                     /gene="repA"
                     /locus_tag="ACHKTW_004070"
     CDS             13267..14124
                     /gene="repA"
                     /locus_tag="ACHKTW_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HFO6464993.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPRKGAGTLKFCEKLMEKAVGFSSR
                     FDFAIHVAHARSRGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESGAGKLSITRATRALTFLSELGLITYQTEYDPLIGCYIPTDITFTPALFAALDV
                     SEDAVAAARRSRVEWENRQRKKQGLDTLGMDELIAKAWRFVRERFRSYQTELKSRGIK
                     RARARRDANRERQDIVTLVKRQLTREIAEGRFTASREAVKREVERRVKERMILSRNRN
                     YSRLATASP"
BASE COUNT         3597 a         3491 c         4305 g         3228 t
ORIGIN      
        1 ctgagcggtt gtgcaaattc tttgtgcgac cgtgaagtgt tgccggcgaa gccggaattt
       61 aacggttatt aatcttttca tcattaataa tcagtccgac tgactttttt tcttcggaat
      121 atcatcatga gttttaacgc aaaggatatg acccagggcg gtcagattgc gtccatgcgt
      181 atccgcatgt tcagccagat cgccaatatc atgctttact gcctgtttat ttttttctgg
      241 atactcgttg gtctggtttt atggataaaa ataagctggc agacgtttgt gaacggctgt
      301 atttactggt ggtgtaccac gctggaaggc atgcgggatt taatcaaatc ccagccggtc
      361 tatgagatcc agtattacgg taaaaccttc cgcatgaacg ctgctcaggt actgcatgac
      421 aaatatatga tctggtgcag tgagcagtta tggtccgcct tcgtgctggc tgctgttgtg
      481 gcactggtta tttgcctgat taccttcttt gttgtctcct ggattctggg gcgtcagggt
      541 aaacaacaga gcgaaaatga agccacaggt ggtcgtcagc tgacagacaa tccgaaagac
      601 gttgcccgga tgctgaaaaa agacggcaag gactccgata tccggattgg agacctgccg
      661 attatccggg attccgaaat ccagaacttc tgtctgcacg gcacggtcgg ggccggtaag
      721 tcggaagtta tccgtcgtct ggccaactac gcccgtcagc gcggtgatat ggtggtgatt
      781 tatgaccgtt cgggggaatt tgttaaaagt tactatgatc cctccatcga taaaatcctg
      841 aatccgctgg atgcccgctg tgccgcctgg gatttatgga aggagtgtct gacacagccg
      901 gattttgata atacggcaaa taccctgatc ccgatgggca caaaagagga cccgttctgg
      961 cagggttcag gacgtaccat ttttgcggaa gcggcgtacc tgatgcgtaa tgaccccaac
     1021 cgtagctaca gcaaactggt ggacacactg ctttccatca aaatcgaaaa actgcgtacc
     1081 ttcctgcgta attcaccggc ggccaacctg gtggaagaga aaattgagaa aacggcgatt
     1141 tccatccgtg ctgtgctgac caactacgtg aaagccatcc gttacctgca ggggattgag
     1201 cataacggtg agtcttttac catccgtgac tggatgcggg gtgtccggga agatcagaaa
     1261 aacggctggc tgtttatctc gtcgaatgcc gacacccatg cctccctgaa gccggtgatc
     1321 tccatgtggc tgtccattgc cattcgtggt ctgctggcaa tgggagaaaa ccgtaaccgt
     1381 cgtgtgtggt ttttctgtga tgagctaccc acgttacaca aactgccgga cctggtggag
     1441 atcctgccgg aagcccgtaa gttcggtggc tgttatgtgt ttggtatcca gtcctatgcc
     1501 cagctggaag atatctacgg tgagaaagcg gctgccacgc tgtttgacgt catgaacacc
     1561 cgtgcctttt tccgttctcc cagccataag attgcagagt tcgctgccgg tgagattggt
     1621 gagaaagagc acctgaaagc cagcgagcag tactcctacg gtgctgatcc ggtacgtgac
     1681 ggggtatcga ccggtaagga tatggagcgc cagacgctgg tcagttattc cgacattcag
     1741 tctctgccgg atttgacctg ttatgtcacc ctgcccggac cgtatccggc agtaaaactc
     1801 tcactgaaat atcaggcacg tccgaaggtt gcccctgagt ttattccgcg tgacatcaac
     1861 ccggaaatgg agaaccgcct gagcgccgta cttgccgcaa gggaagcaga aggtcgtcag
     1921 atggccagcc tcttcgaacc ggatgtgccg gaagttgttt ccggagaaga cgtgactcag
     1981 gctgaacagc cgcaacagcc gcaacagccg caacagccgc aacagccgca acagccgcaa
     2041 cagccgcaac agccgatgtc tcctgccatc aacgataaga agtcagattc aggtgtgaat
     2101 gttccggcag gggggatcga gcaggagctg aaaatgaaac cggaagaaga gatggaacag
     2161 caactgccac ccgggatcag tgaatccggt gaagtggtgg atatggccgc ttatgaggca
     2221 tggcaacagg aaaatcatcc ggacatccag cagcagatgc agcgtcgtga agaggtgaac
     2281 attaatgtgc accgggagcg cggggaggat gttgagccgg gagatgattt ctgatgatga
     2341 gtatcgcgca ggtcagatcg gccggcagtg ccgggaacta ttataccgac aaggataatt
     2401 actatgtgct gggcagcatg ggagaacgct gggccggcag gggggctgaa cagctgggac
     2461 tgcagggcag tgtcgataag gatgttttta cccgtcttct ggagggaagg ctgccggacg
     2521 gagcggatct aagccgcatg caggatggca gtaacaggca tcgtcccggc tacgacctga
     2581 ccttctccgc ccccaaaagt gtctccatga tggccatgtt aggtggcgat aagcgcctga
     2641 ttgatgcaca taaccaggcc gtggactttg ctgttcgtca ggtggaggcg ctggcctcca
     2701 cacgggtgat gacggacgga cagtcagaaa cggtgctgac cggtaatctg gtgatggcac
     2761 tgtttaacca cgacaccagt cgcgatcagg aaccacagtt acacacgcat gcggtggtgg
     2821 ctaatgtcac gcagcataac ggcgaatgga agacactgag cagtgacaaa gtggggaaaa
     2881 cggggttcat tgagaatgtg tacgctaatc agattgcctt tggcaggctc taccgggaaa
     2941 aactgaaaga gcaggttgag gcgctgggct atgaaaccga agtggtgggt aagcacggta
     3001 tgtgggaaat gccgggcgta ccggtggagg ccttttccgg acgctcacag gcaatccggg
     3061 aggccgtcgg ggaagatgcc tcgctgaaat cccgggatgt ggcggcactg gatacgcgaa
     3121 aatccaaaca gcacgtcgat cctgaagtca gaatggctga atggatgcag acgctgaagg
     3181 aaaccgggtt cgacatccgg gcatatcgtg acgcggcgga tcagcgtgcg gagacccgca
     3241 cgcaggcgcc cggggctgtt tcacaggagg ggccggatgt gcagcaggcg gtgacacagg
     3301 ccattgccgg attaagtgaa cgaaaagtgc agtttacgta cacggacgtg ctggccagaa
     3361 cggtcggcat actgccgccg gaaaatggtg tgattgaacg ggcacgcgcc ggtatcgatg
     3421 aggccatcag ccgtgagcag cttatccccc tcgatcgtga gaaggggctg ttcacgtccg
     3481 ggattcatgt gctcgatgag ctgtcagtcc gggcactcag tcgtgacatc atgaaacaga
     3541 accgggtgac cgtacatccg gagaaaagtg tcccccggac ggctggttac agcgatgcgg
     3601 tcagtgttct ggcacaggac cgtccgtcgc tggccattgt gtccgggcag ggcggtgcag
     3661 ccgggcagcg tgagcgggtg gctgaactgg tcatgatggc ccgggagcag gggcgggagg
     3721 tgcagattat cgctgctgac cgtcgctcgc agatgaacct gaagcaggat gaacggttgt
     3781 ccggtgagct gataaccgga cgtcgtcagc tgctggaagg catggccttc acgccgggca
     3841 gtactgttat cgttgaccag ggcgaaaaac tctccctgaa agagacgtta accctgctgg
     3901 acggtgctgc acgtcataac gtacaggtcc tgataaccga cagcgggcag cgaaccggta
     3961 caggcagtgc actgatggcc atgaaggatg ccggggtgaa cacatatcgc tggcaggggg
     4021 gagaacagcg accggccacc atcatcagtg aaccggaccg taatgtccgc tatgcccggc
     4081 tggcaggaga ttttgcggtc agcgtgaaag ccggagaaga gagcgtggca caggtcagcg
     4141 gggtacggga acaggccata ctgacacagg ccattcgcag tgagctgaaa acacagggcg
     4201 tgctcggaca cccggaggta accatgaccg ccctgtcacc ggtctggctg gacagccgga
     4261 gccgttatct gcgggatatg taccgtccgg ggatggtgat ggagcagtgg aacccggaga
     4321 cacgcagtca tgaccgctat gttatcgacc gggtgacggc gcagagtcac agcctgaccc
     4381 tgcgggatgc gcagggtgaa acgcaggtgg tgcgtatttc ctccctggac agcagctggt
     4441 cgctgttccg gccggaaaaa atgccggtgg cagacggcga gcgactgagg gtgacaggga
     4501 aaattcccgg actccgcgtc tccggcggtg accgcctgca ggtggcatcc gtcagtgaag
     4561 atgcgatgac ggttgttgtg ccggggcggg ctgaaccggc caccctgcct gtggctgatt
     4621 caccgttcac ggcactgaag ctggagaacg gctgggtgga aacgcccggg cattccgtca
     4681 gcgacagtgc gaaggttttt gcctccgtca cacagatggc aatggacaac gccaccctga
     4741 acggtctggc ccgcagcggt cgtgatgtcc ggctgtattc ctcactggat gaaacccgta
     4801 ctgcggaaaa acttgcccgc catccctcct ttacggtggt ttctgagcag ataaaggcgc
     4861 gtgccggtga gacatcgctg gaaacggcta tcagtcatca gaagtccgca cttcacacgc
     4921 cggcacagca ggccattcat ctggcccttc cggtggtgga aagtaaaaac ctggccttca
     4981 gccatgtgga cctgctgaca gaggcaaagt cgtttgctgc agaaggaacc agttttactg
     5041 aactggggcg ggaaatcgat gcgcagataa aacggggtga tttactgcat gtggatgtgg
     5101 ctaaaggcta tggcacagac ctgctggttt cccgtgcgtc gtatgaggca gaaaagagca
     5161 ttcttcgcca tattctcgag ggtaaggagg cggtcacgcc gctgatggag agagtacctg
     5221 gcgaactcat ggagaaactg acatcaggac agcgtgccgc cacccgcatg atactggaaa
     5281 cgtccgaccg tttcacggtg gtgcaggggt atgccggggt gggtaagacc acacagttcc
     5341 gggcggtgat gtcagccgtg aacatgctgc cggagagtga gcgtccccgt gtcgttgggc
     5401 tggggcccac gcaccgtgcg gtcggggaga tgcgcagcgc aggcgtggat gcacagacac
     5461 tggcgtcctt tctgcatgac acgcagctgc agcagcgcag cggagaaacg ccggatttca
     5521 gcaacacgct gttcctgctc gatgagagct caatggtggg caataccgac atggcacggg
     5581 catacgccct gattgcggcc ggtggtggtc gtgccgtggc cagcggtgac acggaccagc
     5641 tgcaggccat cgcgcccggt cagcctttcc gtctccagca gacgcgcagt gctgccgatg
     5701 tggccatcat gaaggagatt gtgcgtcaga cgccggaact gcgggaggcg gtatacagcc
     5761 tgattaaccg ggatgtggaa agggcactgt ccgggcttga gagtgtgaaa ccgtctcagg
     5821 tgccacgtct ggagggcgca tgggcaccgg agcactccgt gacggagttc agtcacagcc
     5881 aggaagcgaa actggcagaa gcgcagcaga aagcgatgct gaaaggcgag gcttttccgg
     5941 atgtccccat gacactgtat gaagccattg tccgcgacta taccggcagg acaccggaag
     6001 cacgggagca gacgctgatt gttacgcacc tgaatgagga ccggcgcgtg ctgaacagca
     6061 tgattcatga tgcactggaa aaggccggtg agctgggaaa agagcaggtc atggtgcctg
     6121 tcctgaacac agcgaatata cgcgacgggg agttgcgtcg tctctccacc tgggagacac
     6181 atcgggacgc acttgccctg gtggataatg tgtatcaccg gattgccggt atcagtaagg
     6241 atgacgggct gataaccctg gaggatgcgg aaggtaacac gcggctgatt tcgccccggg
     6301 aggcggtggc tgaaggcgtc acactataca ctccggacac catccgggtg ggaaccggtg
     6361 accggatgcg cttcacgaag agtgaccggg agcgcggtta tgtggccaac agcgtctgga
     6421 cggtgacagc ggtttccggt gacagtgtca cgctgtcgga cggacagcag acccgggaga
     6481 ttcgccccgg ccaggagcag gcagagcaac atattgacct ggcctatgcc atcaccgctc
     6541 acggtgcgca gggggcaagt gaaacctttg ccattgcgct tgaaggcacg gaaggtaacc
     6601 ggaaacagat ggccggcttt gagtcagcct atgtggccct gtcgcgtatg aagcagcatg
     6661 tgcaggtgta caccgataac cgtcagggct ggacggatgc catcaacaat gccgtacaga
     6721 aaggaactgc ccacgatgtg tttgagccga aaccggaccg ggaggtcatg aatgcagagc
     6781 ggttgttcag tacggcgcgg gagctgcggg acgtggcggc aggccgtgcc gttctccgtc
     6841 aggcggggct ggccggggga gacagtcctg cacggtttat tgctccggga cgtaaatatc
     6901 cgcaaccgta tgtggcactg ccggcttttg accgtaacgg caagtccgcc ggtatctggc
     6961 tgaatccgct gaccacggat gacggaaacg ggctgcgggg cttcagtggt gaaggccggg
     7021 tgaaaggcag cggggatgcg cagttcgtgg ccctgcaggg cagccgtaac ggagagagcc
     7081 tgctggctga taatatgcag gatggtgtcc ggattgcccg tgataatcct gacagcggtg
     7141 tggtggtaag aatcgccggt gaaggtcgtc cgtggaatcc cggtaccatc accggtggtc
     7201 gcgtgtgggg ggatatcccg gacaacagcg ttcagccggg agccggaaat ggcgaaccgg
     7261 tcacggcaga ggtactggca cagcggcagg ctgaagaggc catccgccgt gaaacggaac
     7321 gccgtgcgga tgaaattgtc cgtaaaatgg cagagaacaa acctgacctg ccggacggca
     7381 aaacagagca ggctgtcagg gatattgccg ggctggagcg tgaccggtct gccatatctg
     7441 aacgggaagc cgcgctgccg gagagtgtac tgcgtgaacc acaacgggtg cgggaagcgg
     7501 tccgggaggt tgcccgggaa aatctgctgc aggagcgact gcagcagatg gagcgggata
     7561 tggttcgtga cctgcagaaa gagaaaaccc tgggcggaga ctgacacagg aagataaacg
     7621 ctgatgacaa cggataacac gaacacaaca cgtaatgatt cactggctgc ccggaccgat
     7681 acctggttgc agtcgttgct ggtctggtca cccggacagc gggatatcat caaaacggtg
     7741 gcactggtgc tgatggttct ggaccatgca aaccgcattc ggcatctgga ccagtcgtgg
     7801 atgtttctgg ttgggcgtgg ggcctttccg ctgtttgccc tggtgtgggg gctgaatctg
     7861 tcccgtcatg cgcatatccg gcaaccggcc attaaccggc tgtggggatg ggcgatgatt
     7921 gcccagttcg catattatct ggccggcttt ccctggtatg aggggaatat cctgtttgcc
     7981 tttgcagtgg cggcacaggt gctgacatgg tgtgaaacgc gcagtgggtg gcgtactgca
     8041 gcggccattc tcctgatggc actgtggggg cctttgtccg gcaccagtta cggcattgcc
     8101 gggctgctga tgctggcagt cagtcaccgg ctgtaccggg cggaagacag aacggaacgt
     8161 ctggtgctgg ttgcatgcct gctggccgtg attccggcac ttaaccttgc ctccagtgat
     8221 gcggcggctg tagctggtct tgtgatgacg gtgctgaccg ttggtctggt gtcgcgtgta
     8281 ggaaaatcat taccccgttt ctggcccgga gattttttcc cgacgttcta tggctgtcat
     8341 ctggctgtgc tgggcattct ggcgttgtga ctggtatgat actgacgctg attctgctta
     8401 ttaataaaca acataacaca ggatatttat gaaaattacg gatcacaaac tctctgaagg
     8461 tattgccctg acatttcgtg ttcctgaagg aaatataaaa catcctttga ttatcttatg
     8521 ccatgggttt tgcggtatca ggaatgttct gctcccttgc tttgcgaatg ccttcacaga
     8581 agcaggattc gcaacgatta cttttgatta tcgaggattc ggtgagagtg atggagaacg
     8641 aggtcgtctg gttcctgcaa tgcagaccga agatattatt tcagttatca attgggcaga
     8701 aaagcaggaa tgtattgata atcagcgtat tgggttatgg ggaacatcgc tgggaggggg
     8761 gcatgtcttc agtgcagctg cgcaggacca acgcgtaaaa tgtattgtca gccagctggc
     8821 ttttgctgac ggtgaggttc tggttaccgg agagatgaat gaatcggaga gagcgtcttt
     8881 tctatccacg ctgaataaaa tggcagagaa gaagaaaaac accgggaaag agatgtttgt
     8941 gggggttaca cgggtactaa gtgatgatga atcgaaggta ttcttcgaga aaatcaaagc
     9001 ccggcatccc gaaatggata taaaaatacc tttccttacg gtcatggaaa cgttgcaata
     9061 taaaccggca gagagcgcag ccaaagtaca gtgcccagtt ttggttgtta ttgccggcca
     9121 ggatagcgtt aatccaccgg aacagggaag agcattatat gatgctgttg cttctggtac
     9181 taaggaatta tacgaggaag ctgatgcatg ccattatgat atttatgagg gcgcgttttt
     9241 tgaacgtgta gtagctgtgc agacacaatg gtttaaacaa tatctttaat tgtcagtatt
     9301 tatacgttaa aggtcgttga aattaacttc ctgttacgat gagttgtttc aacggcctgc
     9361 agggcatatt ttcacgaagg tcagagtaag catggcagag caaaaacgac cggtactgac
     9421 actgaagcgg aaaacggaag gagagacgcc tgtccgcagc cggaaaacca tcatcaatgt
     9481 caccacgcca ccaaaatgga aggtgaaaaa gcagaaactg gcggagaagg ctgcccggga
     9541 agcagagctg gcggcgaaga aagcacaggc cagacaggcg ctgtccattt atctgacgct
     9601 gccctcgctg gatgaggcgg tgaacactct gaaaccctgg tggccgggat tgtttgacgg
     9661 taacacgccc cggcttcttg cctgtggtat ccgggaagtt ttactggatg aggtgtctca
     9721 gcggaatatc ccgctctctc ataaaaaact gcgcagggca ctgaaggcca tcacccgttc
     9781 agaaagctat cttggcgcca tgaaagccgg tgcctgccgc tatgatacag aaggctatgt
     9841 gacagaacat atcacacagg aagaagaaca gtatgcgcag gcgcgcctgg aaaaggtgcg
     9901 cagacagaac cggatcaagg atgagctgcg ggcgatcctg gcagaataag aaaagcgccc
     9961 tctgtatgac agagggcaac agcgaaaaaa atcgggaaaa cagacatcca gagttttgat
    10021 ggagaaaaac atccaaccgt tcagggatgt ttcataatgt ttctgacata atccgggtgt
    10081 cgggtcaaga ggagggataa aaaatggctg tatccagtgg attatctgaa cacagcagta
    10141 tttatcgcta tcagcgcgag gggcgtcatg ccgtccgtca ggagattcgt ggctttttca
    10201 gggaagcggc agaggtctgg cagaatgcgg cccggctggc accctgtgaa gcatggcggg
    10261 attttgctct gaagagggca tctgcatgtc ggtgtaagtg ccgataaaaa taacccccgt
    10321 cgcggagctc cgggggatca gacttatatg gcagatcgtt attattatta tagtggtgta
    10381 agaaggagtt gccctgtctt tcaaacggac atcacagaat taacatattt cacatcccgg
    10441 gtaagccagc agggataaaa aatgtgatga atttcaagaa gcaggaggtg acagtggcat
    10501 gcataacagt agggttatcc ggtgaatctg acagacagcc gggctgtatt cgcggcagga
    10561 taaagaaaaa ccgggagatg actctcccgg tccgtcttat cgctaatccc ctgagcaggg
    10621 catgtatgac ttatctatga ccgttatatt cagcgccaca tccggtagcc tgcgatataa
    10681 cacagttatc ctgtcagcaa gagcaggtat gatgaataca cggagattaa aaatataatc
    10741 tccgtcacat gttacccacg gaaaccggta acgccttcac gggaaatatg tgacagaaaa
    10801 agcagcctcc gttactccag gtatcaggag gctgaataat gcatgagacg ttgtttctta
    10861 tgtgtctggt caggctggct gaccagcgtt tttagtaaca catagccata tgtgtattaa
    10921 gtcacctcat cttattttgt tgaattgatc acaactcaac ggtttatttt ttgcgtgata
    10981 cgtgagccat tattcatccc ggcatggaat tcatgcaatc acgaaaagta atcagtgacg
    11041 gagagtaaaa tgaagaaata tataccagtg gtattatttg ttttttcatg tcaggtattt
    11101 tctgctgata ttcatggacg ggtcgtgcgt gttctggatg gcgataccat tgacgtcatg
    11161 ttatctcagc atccggtgcg tgtccgtctt gttaatattg atgcacctga aaagaaacag
    11221 gaatatggac ggtggtctga aaaaataatg aaatctctgg ttgcgggaaa aacggttact
    11281 gtcacatatt ttcagcgaga tcattatggt cgtattctcg ggcaggttta tgcaccagac
    11341 ggaatgaatg ttaatcagtt tatggtccgt gcaggcgatg catgggttta tgagcagtac
    11401 aacaccgatc ctgttcttcc tgtcctgcag ggagaagtac gacagcagaa acgtggtctg
    11461 tgggcagaca gcgctcctgt tccgccctgg gtatggcgct cacggaaata atgatatttc
    11521 tgtggctatt gagttctcat tgaaatgcga tatttaaaag catgaagcat attacattat
    11581 taacttattt ctctgttaat gaaaggtgaa caaatgaact atcaggcagt atcagaatta
    11641 atcacatcgt ctaatcataa tgttcttata gtgggggaat cagcttctga agttgatagc
    11701 tttttaaata aactgaatat cactgattat aagtattatg atttcagcca gatttacagt
    11761 tgttctgaca gaacattaaa tgattatgct gttattttta tcaggaatgc gctgaacgcc
    11821 agtgagcata ttattatttt taattgtaca ggatggtctg atttgaataa tgaatctgcc
    11881 gtcatgcagt ttgccagagt ggcacgaaaa tcaggaaaac aactgatagt cgcagtaaga
    11941 gaacaggata tgaaaaaaat ggaagcagag tttggaagga tcataaaaat ccactgacgc
    12001 cttcgcgttg ctcagttgtc caaccccggg gacgggaaaa agcaagtttt ccccgccccc
    12061 ggcttttctg tagtgaaaag cgggaaatgg agggaatggt cagtaacagg gaatgtggta
    12121 ttatcgcggc gggtgtctga gcctttctgg ttcaggcaag acgcaggtac cagaaatgcg
    12181 aagaccccac ttgttaatcc attaactcgt gaggcctgca tgaagtacct taacactact
    12241 gattgtagcc tcttccttgc agagaggtca aagtttatga cgaaatatgc ccttatcggg
    12301 ttgctcgccg tgtgcgccac ggtgttgtgt ttttcactga tattcaggga acggttatgt
    12361 gagctgaata ttcacagggg aaatacagtg gtgcaggtaa ctctggccta cgaagcacgg
    12421 aagtaagctg ccgggcgggg agggaagtcc ccgctttccg gaagtgtgag gtatttcagg
    12481 ggcagacacc cgacatgcca gaaacagccg gtcccgcccg gggccggcac acaggttaag
    12541 gcatttccat cttttcagtc atttcattat caaaatcaca ttaaacggtc gtaatcagac
    12601 atgatttgtg cgccaacaca gatcatcgtc acaactttca agtcgctgat ttcaaaaaac
    12661 tgtagtatcc tctgcgaaac gatccctgtt tgagaattga ggaggcgaga tgtcgcagac
    12721 agaaaatgca gtgacttcct catcaggtac aaagcgagca tacagaaaag gtaatcctct
    12781 gacgcttgca gaacgtcagc aggcatccct ggcaagaaaa cgagccacac ataaagaact
    12841 cagagtgttt attcctgctg cgttaaaagc gcaattgcag gagatgtgtg aagctgaggg
    12901 tgttactcag gctgagatga ttgccgaact gataaagcag aagagtgctt ttagctaact
    12961 gaaatgtagc gacatcacat tcttgcctgt ttctggtgtg cgtgatagat tgctgatcgt
    13021 ttaaggaatt ttatggctgg ccacgccata aggtggcagg gaactggttc tgatgtggat
    13081 gtacaggagc cagaaaagca aaaaccccga taatctttct atgtttggcg acgagaagat
    13141 taccggggcc ccataaaacc gcatagccaa caattcagct atgcggggag tatagttata
    13201 tgcccggaaa agttcaagac ttctttctgt gctcactcct tctgcgcatt gtaagtgcag
    13261 gatggtgtga ctgatcttca acaaacgtat taccgccagg taaagaaccc gaatccggtg
    13321 tttacacccc gtaaaggtgc cggaacgctg aagttctgcg aaaaactgat ggaaaaggcg
    13381 gtgggcttct cctcccgttt tgatttcgcc attcatgtgg cgcatgcccg ttcgcgtggt
    13441 ctgcgtcggc gcatgccacc ggtactgcgt cgccgggcca ttgatgcgct gctgcagggg
    13501 ctctgttttc actatgatcc gctggccaac cgcgtccagt gctccatcac cacgctggcc
    13561 attgagtgcg gactggcgac agagtccggt gcaggaaaac tctccatcac ccgtgccacc
    13621 cgggccctga cgttcctgtc agagctggga ctgattacct accagacgga atatgacccg
    13681 cttatcgggt gctacattcc gaccgacatc acgttcacac cggctctgtt tgctgccctt
    13741 gatgtgtctg aggatgcagt ggcagctgcg cgccgcagtc gtgttgaatg ggaaaacaga
    13801 cagcgcaaaa agcaggggct ggataccctg ggtatggatg aactgatagc gaaagcctgg
    13861 cgttttgtgc gtgagcgttt ccgcagttac cagacagagc ttaagtcccg tggaataaaa
    13921 cgtgcccgtg cgcgtcgtga tgcgaacagg gaacgtcagg atattgtcac cctggtgaaa
    13981 cggcagctga cgcgtgaaat cgcggaagga cgtttcactg ccagtcgtga ggcggtaaaa
    14041 cgcgaagtgg agcgtcgtgt gaaggagcgc atgattctgt cacgtaaccg taattacagc
    14101 cggctggcca cagcttcccc ctgaaagtga cctcatcaga ataatccggc acgcgccgga
    14161 ggcattcgca cgcctgaagc ccgccagtgc acaaaaaatc agcaccacat acaaaaaaca
    14221 acctcatcat ccaccttctg gtgcatccgg tcccccctgt ttttgataca aaacacgcct
    14281 cacagacggg ggattttgct tatccacatt taactgcaag ggacttcccc ataaggttac
    14341 aaccgttcat gtcataaagc gccagccgtc agccttacag ggtgcaatgt atcttttaaa
    14401 cacctgttta tatctccttt aaactaccta attacattca tttaaaaaaa aacctattca
    14461 ctgcctgtcc tgtggacaga cagatatgca cctcccaccg caagcggcgg gccccgatcg
    14521 gagccaattt agttacaaca ttcaaacata gactccggaa aaaccccggt tccgcgcaga
    14581 actgaaacca caaagcccct cctcataatt aataagcgcc g
//
LOCUS       DAZFQD010000134        14459 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.134, whole genome shotgun sequence.
ACCESSION   DAZFQD010000134 DAZFQD010000000
VERSION     DAZFQD010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14459
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.134"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            195..1766
                     /gene="garD"
                     /locus_tag="ACHKTW_004071"
     CDS             195..1766
                     /gene="garD"
                     /locus_tag="ACHKTW_004071"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008867 - galactarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0046392 - galactarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HFO6464994.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPAPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QLERLLVGTDDVQAIPVESASIVSLQDEKHVGFQSMVEDILQVAERHLQKLNQRQRET
                     CPASELVVGMQCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMATRTELANRWFDLMDINAGTIATGEETIEEVGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            1915..2250
                     /gene="prlF"
                     /locus_tag="ACHKTW_004072"
     CDS             1915..2250
                     /gene="prlF"
                     /locus_tag="ACHKTW_004072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin PrlF"
                     /protein_id="HFO6464995.1"
                     /translation="MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHY
                     EILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNNPQKTRPFNIQQGKKLVAGMDVN
                     IDDEIGDDE"
     gene            2250..2714
                     /gene="yhaV"
                     /locus_tag="ACHKTW_004073"
     CDS             2250..2714
                     /gene="yhaV"
                     /locus_tag="ACHKTW_004073"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ribonuclease
                     toxin YhaV"
                     /protein_id="HFO6464996.1"
                     /translation="MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKA
                     ATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKNKDWSRVKFGAGRYRLFFRYSEK
                     EKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH"
     gene            complement(2769..3578)
                     /gene="agaR"
                     /locus_tag="ACHKTW_004074"
     CDS             complement(2769..3578)
                     /gene="agaR"
                     /locus_tag="ACHKTW_004074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HFO6464997.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKHTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLNRRMCEVAERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGE"
     gene            3827..5107
                     /gene="kbaZ"
                     /locus_tag="ACHKTW_004075"
     CDS             3827..5107
                     /gene="kbaZ"
                     /locus_tag="ACHKTW_004075"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaZ"
                     /protein_id="HFO6464998.1"
                     /translation="MKHLTEMVRQHKAGKTNGIYAVCSAHPLVLEAAIRYASANQTPL
                     LIEATSNQVDQFGGYTGMTPADFRGFVCQLADSLNFPQDALILGGDHLGPNRWQNLPA
                     AQAMANADDLIKSYVAAGFKKIHLDCSMSCQDDPIPLTDDIVAERAARLAKVAEETCL
                     EHFGEADLEYVIGTEVPVPGGAHETLSELAVTTPDAARATLEAHRHAFEKQGLNAIWP
                     RIIALVVQPGVEFDHTNVIDYQPAKASALSQMVENYETLIFEAHSTDYQTPQSLRQLV
                     IDHFAILKVGPALTFALREALFSLAAIEEELVPAKACSGLRQVLEDVMLDRPEYWQSH
                     YHGDGNARRLARGYSYSDRVRYYWPDSQIDDAFAHLVRNLADSPIPLPLISQYLPLQY
                     VKVRSGELQPTPRELIINHIQDILAQYYTACEGQ"
     gene            5130..5603
                     /gene="agaV"
                     /locus_tag="ACHKTW_004076"
     CDS             5130..5603
                     /gene="agaV"
                     /locus_tag="ACHKTW_004076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HFO6464999.1"
                     /translation="MPNIVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQ
                     QNLMEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRI
                     NVGNMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGVPTEPAVDLFKLL"
     gene            5614..6393
                     /gene="agaW"
                     /locus_tag="ACHKTW_004077"
     CDS             5614..6393
                     /gene="agaW"
                     /locus_tag="ACHKTW_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIC"
                     /protein_id="HFO6465000.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGGTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGAEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAAAW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPAAQKEEFEDGI"
     gene            6383..7261
                     /gene="agaE"
                     /locus_tag="ACHKTW_004078"
     CDS             6383..7261
                     /gene="agaE"
                     /locus_tag="ACHKTW_004078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019079236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IID"
                     /protein_id="HFO6465001.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDQSIGAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFIVLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQTDVIDKLMPAFLPLVYTLTMFWLVRRGWSPLRLI
                     AVTVVLGIVGKFCHFL"
     gene            7279..7713
                     /gene="agaF"
                     /locus_tag="ACHKTW_004079"
     CDS             7279..7713
                     /gene="agaF"
                     /locus_tag="ACHKTW_004079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine/N-acetylgalactosamine
                     transporter subunit IIA"
                     /protein_id="HFO6465002.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGEQSQFIAIDFPETSSTALL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLAMQKPGCEVITGTNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTSLVDELGRCHEECPVEEGI"
     gene            7710..8843
                     /gene="nagA"
                     /locus_tag="ACHKTW_004080"
     CDS             7710..8843
                     /gene="nagA"
                     /locus_tag="ACHKTW_004080"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFO6465003.1"
                     /translation="MTHVLRARRLLTEEGWLDDHQLRIADGVIAAIEPIPVGVTERDA
                     ELLCPAYIDTHVHGGAGVDVMDDAPDVLDKLAMHKAREGVGSWLPTTVTAPLNSIHAA
                     LKRIAQRCQRGGPGAQVLGSYLEGPYFTPQNKGAHPPELFRELEIAELDQLIAVSQHT
                     LRVVALAPEKEGALQAIRHLKQQNVRVMLGHSAATWQQTRAAFDAGADGLVHCYNGMT
                     GLHHREPGMVGAGLTDKRAWLELIADGHHVHPAAMSLCCCCAKERIVLITDAMQAAGM
                     PDGRYTLCGEEVQMHGGVVRTASGGLAGSTLSVDAAVRNMVELTGVTPAEAIHMASLH
                     PARMLGVDGVLGSLKPGKRASIVALDSGLHVQQIWIQSQLASF"
     gene            9194..10348
                     /locus_tag="ACHKTW_004081"
     CDS             9194..10348
                     /locus_tag="ACHKTW_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgaS family sugar isomerase"
                     /protein_id="HFO6465004.1"
                     /translation="MPENYTPAAAATGTWTEEEIRHQPRAWIRSLTNIDALRSALNNF
                     LEPLLRKENLRIILTGAGTSAFIGDIIAPWLASHTGKNFSAVPTTDLVTNPMDYLNPA
                     HPLLLISFGRSGNSPESVAAVELANQFVPECYHLPITCNEAGALYQNAINSDNAFALL
                     MPAETHDRGFAMTSSITTMMASCLAVFAPETINSQTFRDVADRCQAILTSLGDFSEGV
                     FGYAPWKRIVYLGSGGLQGAARESALKVLELTAGKLAAFYDSPTGFRHGPKSLVDNET
                     LVVVFVSSHPYTRQYDLDLLAELRRDNQAMRVIAIAAESTDIVAAGPHIILPPSRHFI
                     DVEQAFCFLMYAQTFALMQSLHMGNTPDTPSASGTVNRVVQGVIIHPWQA"
     gene            10361..11221
                     /gene="kbaY"
                     /locus_tag="ACHKTW_004082"
     CDS             10361..11221
                     /gene="kbaY"
                     /locus_tag="ACHKTW_004082"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaY"
                     /protein_id="HFO6465005.1"
                     /translation="MSIISTKYLLQDAQANGYAVPAFNIHNAETIQAILEVCSEMRSP
                     VILAGTPGTFKHIALEEIYALCSAYSTTYNMPLALHLDHHESLDDIRRKVHAGVRSAM
                     IDGSHFPFAENVKLVKSVVDFCHSQDCSVEAELGRLGGVEDDMSVDAESAFLTDPQEA
                     KRFVELTGVDSLAVAIGTAHGLYSKTPKIDFQRLAEIREVVDVPLVLHGASDVPDEFV
                     RRTIELGVTKVNVATELKIAFAGAVKAWFAENPQGNDPRYYMRVGMDAMKEVVRNKIN
                     VCGSANRISA"
     gene            11388..11864
                     /gene="agaB"
                     /locus_tag="ACHKTW_004083"
     CDS             11388..11864
                     /gene="agaB"
                     /locus_tag="ACHKTW_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IIB"
                     /protein_id="HFO6465006.1"
                     /translation="MSSPNILLTRIDNRLVHGQVGVTWTSTIGANLLVVVDDVVANDD
                     IQQKLMGITAETYGFGIRFFTIEKTINVIGKAAPHQKIFLICRTPQTVRKLVEGGIDL
                     KDINVGNMHFSEGKKQISSKVYVDDQDLADLRFIKQRGVNVFIQDVPGDQKEQIPD"
     gene            11903..12706
                     /gene="agaC"
                     /locus_tag="ACHKTW_004084"
     CDS             11903..12706
                     /gene="agaC"
                     /locus_tag="ACHKTW_004084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IIC"
                     /protein_id="HFO6465007.1"
                     /translation="MHEITLLQGLSLAALVFVLGIDFWLEALFLFRPIIVCTLTGAIL
                     GDIQTGLITGGLTELAFAGLTPAGGVQPPNPIMAGLMTTVIAWSTGVDAKTAIGLGLP
                     FSLLMQYVILFFYSAFSLFMTKADKCAKEADTAAFSRLNWTTMLIVASAYAVIAFLCT
                     YLAQGAMQALVKAMPAWLTHGFEVAGGILPAVGFGLLLRVMFKAQYIPYLIAGFLFVC
                     YIQVSNLLPVAVLGAGFAVYEFFNAKSRQQAQPQPVASKNEEEDYSNGI"
     gene            12696..13487
                     /gene="agaD"
                     /locus_tag="ACHKTW_004085"
     CDS             12696..13487
                     /gene="agaD"
                     /locus_tag="ACHKTW_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IID"
                     /protein_id="HFO6465008.1"
                     /translation="MGSEISKKDITRLGFRSSLLQASFNYERMQAGGFTWAMLPILKK
                     IYKDDKPGLSAAMKDNLEFINTHPNLVGFLMGLLISMEEKGENRDTIKGLKVALFGPI
                     AGIGDAIFWFTLLPIMAGICSSFASQGNLLGPILFFAVYLLIFFLRVGWTHVGYSVGV
                     KAIDKVRENSQMIARSATILGITVIGGLIASYVHINVVTSFAIDSTHSVALQQDFFDK
                     VFPNILPMAYTLLMYYFLRVKKAHPVLLIGVTFVLSIVCSAFGIL"
     gene            13488..14243
                     /locus_tag="ACHKTW_004086"
     CDS             13488..14243
                     /locus_tag="ACHKTW_004086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosamine-6-phosphate isomerase"
                     /protein_id="HFO6465009.1"
                     /translation="MERGTASGGASLLKEFHPVQTLQQVENYTALSERASEYLLAVIR
                     SKPDAVICLATGATPLLTYHYLVEKIHQQQVDISQLTFVKLDEWVDLPLTMPGTCETF
                     LQQHIVQPLGLREDQLISFRSEEINETECERVTNLIARKGGLDLCVLGLGKNGHLGLN
                     EPGESLQPACHISQLDARTQQHEMLKTTGRPVTRGITLGLKDILNAREVLLLVTGEGK
                     QDATERFLTAKVSTAIPASFLWLHSNFICLINT"
BASE COUNT         3514 a         3650 c         3751 g         3544 t
ORIGIN      
        1 acataaggtt gcgcgctaaa gcacagattt gcgctttacc ttgccgggcg gcactgcaat
       61 ccctgaaatg attgacattg atcacatttc tgcgtttaaa ctcctgacat tcttatttca
      121 cccaatgaag tcatttattt ttaaatgaga ccaggtcctc attttaataa cccctggctg
      181 gagaatatag tacaatggcc aacatcgaaa tcagacaaga aacgccaact gcgttttata
      241 taaaagttca cgacacagat aatgtggcaa ttattgttaa tgataatggc ctgaaagcag
      301 gaacgcgttt tccggatgga ctggaattaa ttgaacatat tccccagggg cataaagtcg
      361 cattgctgga cattccggct aatggtgaaa ttattcgtta tggtgaagtg attggttacg
      421 ccgtgcgtgc aatcccacgc ggaagctgga tcgacgaatc aatggttgta ctaccggaag
      481 cgccgccgtt acacacgctg ccactggcaa ccaaagtccc ggccccctta ccgccgctgg
      541 aaggatacac ctttgagggc tatcgcaatg ccgatggcag cgtgggcacc aaaaacctgc
      601 tcggcatcac caccagcgtc cactgtgtgg caggcgtggt ggactatgta gtaaaaatca
      661 ttgaacgcga tctgctaccg aaatacccga acgtcgatgg cgtggtgggg ctgaatcatt
      721 tgtacggctg tggcgtggcg attaacgcac cggcggcagt tgtgcctatt cgtaccatcc
      781 acaatatttc gctgaaccct aactttggcg gcgaagtaat ggtgattggt ctgggttgtg
      841 aaaagttgca gcttgagcgc ctgctggtcg gcacggatga tgtgcaagct attccagtag
      901 aaagcgccag cattgtcagt ttgcaggatg aaaagcatgt cggttttcag tccatggtcg
      961 aggatatttt gcaggtcgcc gaacgccatc tacaaaaact gaatcaacgt cagcgagaaa
     1021 cctgtccggc ttcagaactg gttgtcggca tgcagtgcgg tggcagcgat gccttttctg
     1081 gcgtaacggc aaacccggcg gttggctatg cgtctgatct actggtgcgc tgcggcgcaa
     1141 cggtgatgtt ctcagaagtc acggaagtgc gtgacgcgat ccatctgctg acaccacgcg
     1201 cagtgaacga agaggtcggc aaacggctgc tggaggagat ggagtggtac gataactatc
     1261 tcaatatggg aaaaaccgac cgcagcgcca acccttcgcc gggcaacaag aaaggcggtc
     1321 tggcaaacgt ggtagagaag gcactcggct ccattgctaa atcgggtaaa agcgcaattg
     1381 ttgaagtgct gtcgcccggt caacgcccga ctaaacgcgg attaatttac gccgcgacgc
     1441 cagccagcga ttttgtctgt ggcacgcaac aggtggcttc gggtatcacc gtgcaagtgt
     1501 ttacgaccgg tcgtggtacg ccgtacggcc tgatggcggt acccgtcatt aaaatggcga
     1561 cccgcaccga gctggcgaac cgctggtttg atttaatgga tattaacgcg ggcactatcg
     1621 ccaccggcga agaaaccatt gaagaggtgg gctggaagtt gttccacttt attctcgacg
     1681 tcgccagcgg gaagaagaaa accttctcgg atcaatgggg gctgcataac cagctggcag
     1741 tgtttaaccc ggcaccggtg acctgatttc ttttggaacg tccctcgcaa aacatggcct
     1801 tagtgccatg tttttattgt ttaaagcccc cacgtccatt aataatgcat ttgcattacc
     1861 ttagttcaag cttataattt gagcagaaaa caggctgtaa aaggacagtg aatcatgccc
     1921 gctaatgctc gctctcacgc tgtactgacc actgaatcaa aggtcacgat acgcggacaa
     1981 acaactatcc ccgcgccagt gcgtgaggcc ttaaaactga agccaggcca ggacagcatt
     2041 cattacgaaa ttctgcctgg tgggcaagtc tttatgtgcc gactgggaga tgaacaggag
     2101 gatcatacta tgaatgcatt tttgcgtttt ctggatgcag atatccagaa caacccgcaa
     2161 aaaactcgtc cattcaacat tcaacaagga aagaaacttg tcgctggcat ggacgtcaac
     2221 attgatgatg agattggcga cgacgaataa tggattttcc acaaagggtt aatggttggg
     2281 cgctatatgc tcatccctgt tttcaggaaa cctacgacgc tttagttgcc gaagtcgaga
     2341 cattaaaggg aaaagatcct gaaaattatc agagaaaagc cgccacaaag ttattggcgg
     2401 tagtccataa agtgattgag gagcatatca cggtcaatcc atcatcaccg gcattccgtc
     2461 atggcaagtc gttaggctct gggaaaaata aagactggtc acgggtaaaa tttggtgctg
     2521 gtcgttatcg tctcttcttt cgttatagtg aaaaagagaa agtcatcatt ctgggatgga
     2581 tgaacgatga aaacactctg cgcacctacg gtaaaaaaac agatgcctat accgtattca
     2641 gcaaaatgtt aaaaagagga catcctcctg ccgactggga aagcctcacc caagagacag
     2701 aagaaaacca ttgatggtgt tcacaatgcc cgccgaacgt attcgtgttg accagcgggc
     2761 accctttctc actccccgac cagaatcact tcaaccccag cctttcgcag tccttccaga
     2821 ctatccgcag gaatgccttc atcaacaatg atcatgtcga tacgttgagt atcaatgatc
     2881 ttatgtaaac tggaacgatt gaatttactg gaatcggtga ccacgatgat ccgttccgca
     2941 acttcgcaca tccgacggtt taaacgggct tcatcttcat tatgtgtgct gacgccgcgc
     3001 tccagatcga tcgcatctac accaagaaac agcatatcga agtggtaatt ttgcagcgat
     3061 tgctcagcct gatcgccgta aaaagattgc gactgacggc gcaaatgccc gccggtcatc
     3121 agcagctcaa cgccttccgc ttccagcaac gcattagcca cgttcatacc gttggtcatc
     3181 gcaattacgt cagtgtgctt acgcatcaga cgagcaatct caaaagtggt agtcccggaa
     3241 tcgaggatca cccgatgacc aggctgaatc aactcaacgg cagctttcgc aacgctgcgt
     3301 ttcatcgcgg tgttcagcgc gcttttatct tccactgatg gctcgactga cggcgtcgtg
     3361 ctatcgcaga tcaacgcgcc accataggca cgcacagcga tcccctgctt ttccagaaac
     3421 gccagatcat tgcggatcgt cacagtagat acgccataca atgccgacag atcgttaacc
     3481 tgcacactcc cttgctgtcg cagacgctga atgatctgtt ctcgtcgctc gctggtgcca
     3541 gtcactcgct tctcacctga agcgtcggta ttactcatag taagtccttt cgtaaaactt
     3601 tcgtttcatt tcgttttgcc tattaacgcc tttctattaa gcaaatgcaa gcccaccttg
     3661 cccattgacg caagctactc tcgtttcagt gactttcatt atgtttcttt tgtgaatcag
     3721 atcagaaaac tattatcttt cgttttattt ttatctcacc atgacgcagt atcaactgaa
     3781 acaaaacgaa agattaatat cgcagtaatc tgaactggag aggaaagtga aacatctgac
     3841 agaaatggtg agacagcaca aagcgggcaa aacaaatgga atttatgccg tttgttccgc
     3901 acatccgctg gtgctggagg ctgcaatccg ctacgccagt gcaaaccaaa cgccgttact
     3961 gattgaagca acctccaatc aggtagacca gttcggcggt tataccggaa tgacgcccgc
     4021 cgattttcgc ggctttgttt gtcagctcgc cgactcgttg aatttcccgc aggatgcgtt
     4081 gattctgggt ggtgaccatc tggggccaaa ccgctggcaa aacctgccgg ccgctcaggc
     4141 aatggccaat gccgatgatt tgattaaaag ctacgttgcg gcaggattca aaaaaatcca
     4201 ccttgattgc agcatgtcct gtcaggacga tccgattccc ttaactgatg acatcgtggc
     4261 tgaacgcgcc gcccgtctgg cgaaagtggc ggaagaaacc tgtcttgaac actttggcga
     4321 agccgatctg gagtatgtca ttggtaccga agtgccggta cctggcggcg cgcatgaaac
     4381 cttaagcgag ctggcggtca ccacgccgga tgccgcccgc gccacgctgg aagcccatcg
     4441 tcacgccttt gaaaagcaag gtttgaatgc catctggcca cgcatcattg ccctggtggt
     4501 tcaacccggc gtcgaattcg atcacaccaa cgttattgat tatcagcccg ccaaagcgag
     4561 cgccttaagc cagatggtcg aaaactacga aacgctgatt ttcgaagcgc actctaccga
     4621 ttatcaaacg ccgcaatcgc tgcgccagct ggtgattgac cactttgcca ttctgaaagt
     4681 tggcccagcg ctgaccttcg ccctgcgtga agctctgttc tctctggcgg cgattgaaga
     4741 agaactggtg ccagcgaaag cctgttctgg tctgcgtcag gtgctggaag acgtgatgct
     4801 cgaccgcccg gaatactggc aaagccacta ccacggtgac ggcaacgcgc gtcgtctggc
     4861 gcgtggttat agctactcgg atcgcgtgcg ctattactgg ccggacagcc agattgatga
     4921 cgctttcgct catctggttc gtaatctggc ggattcacca attccgctac cgctcatcag
     4981 ccagtatctg ccgctgcaat acgtgaaagt tcgctccggc gagctgcaac cgacgccacg
     5041 ggaactcatt atcaaccata ttcaggacat cctggcgcag tactacacag cctgtgaagg
     5101 ccaataagca aaacaaagag gaacacgcta tgccaaatat tgttttaagc cggattgatg
     5161 aacgcttgat tcacggtcag gtcggcgttc agtgggtcgg atttgcgggg gcaaatctgg
     5221 tgctggtagc caacgatgag gttgccgaag atccggtaca acaaaacctg atggaaatgg
     5281 tactggcaga agggatcgcc gtacgtttct ggacgctgca aaaagttatc gacaacattc
     5341 atcgcgccgc cgatcgacag aaaatcctgc tggtttgtaa aacacccgcc gatttcctga
     5401 cgctggtgaa aggtggcgtt ccggtgaatc gcattaacgt tggcaatatg cactacgcca
     5461 atggcaaaca acaaatcgcc aaaacggttt ctgtggatgc gggcgatatc gcagcattta
     5521 acgacctgaa agccgctggg gtggaatgct tcgttcaggg cgtcccgaca gagcctgctg
     5581 tggacctctt taaattactt tgagggattc atcatggaaa tcagcctgtt gcaggcattt
     5641 gcgttgggca ttatcgcctt tatcgctggc ctggatatgt ttaacggcct aacacatatg
     5701 caccgcccgg tggtcctcgg cccgttggtc gggctggtac ttggcgatct gcataccgga
     5761 attttaaccg gcggtacgct ggaactggtg tggatggggc tggccccgct ggcgggcgca
     5821 cagccgccta acgtgattat cggtactatc gtcggcacgg cgtttgccat tactactggc
     5881 gtgaaacccg atgtcgcggt cggtgtcgcc gtacctttcg ctgtcgcagt acagatgggg
     5941 attaccttcc tgttctcggt gatgtctggc gtgatgtctc gctgcgaccg gatggcggag
     6001 aacgccgata ctcgcggcat tgaacgcgtg aactatctgg cactgctggc actcggcact
     6061 ttctattttc tctgcgcttt cctgcctatc tacttcggcg cggaacatgc aaaaaccatc
     6121 attgatgtcc tgccgcaacg attaatcgac ggcctcggcg tcgcgggcgg catcatgcca
     6181 gcaatcggtt ttgccgtgct gctgaaaatc atgatgaaaa acgtctacat cccctacttc
     6241 atcctgggct ttgttgccgc agcctggctc aagttaccgg tgctggctat tgctgccgct
     6301 gcgctggcga tggcgctgat cgacctgctg cgtaaatctc ctgaaccgac tcaacctgcg
     6361 gcacagaaag aggaatttga agatggcatc taatcaaacc accctgccga acgtcagtga
     6421 aaacgaagaa acattgctga ctggcgtcaa tgaaaacgtg tatgaagatc agagcattgg
     6481 cgcggagctg acgaaaaaag atatcaaccg cgtcgcctgg cgttccatgc tgttacaagc
     6541 gtcttttaac tacgaacgta tgcaggcttc tggctggttg tacggtctgc tgcctgctct
     6601 gaaaaagatc cacactaata aacgcgacct ggcgcgcgcc atgaaggggc atatgggttt
     6661 cttcaatacc catccgtttc tggtgacatt tgttatcggc attatccttg cgatggagcg
     6721 ttctaagcag gacgttaaca gtattcagag caccaaaatt gccgtcggtg cgccgctcgg
     6781 cgggattggt gatgcgatgt tctggctgac gctactgccg atttgtggcg ggataggcgc
     6841 aagccttgcg ctacaaggct ctattcttgg cgcagtcgta tttattgtgc tgttcaacgt
     6901 ggtacacctg gggctgcgtt ttggtctggc gcattatgct taccgcatgg gcgtggcggc
     6961 gattccactc attaaagcga ataccaaaaa agtcggccat gctgcgtcta tcgttgggat
     7021 gacggtaatc ggcgcgctgg tggcaaccta tgttcgttta agcaccacgc tggaaatcac
     7081 tgcgggcgac gcagtggtta agttacagac tgatgttatt gacaaactga tgcctgcctt
     7141 cttaccgctg gtctacacct tgaccatgtt ctggctggta cgccgcggct ggagtccgct
     7201 gcgtctgatt gcagttaccg tggttctcgg catcgtcggt aaattctgcc atttccttta
     7261 aatacaatga ggtattagat gttaagtatt attttgacag ggcatggcgg gtttgccagc
     7321 ggcatggaaa aagcaatgaa gcaaattctc ggtgagcaat cgcagtttat cgccatcgat
     7381 tttccggaaa cctcaagcac cgcgctgctt acatcgcagc ttgaagaggc catcgcccaa
     7441 ctggattgtg aagacggcat tgtttttctg acggatttgc tgggcggcac gccgtttcgc
     7501 gtagcttcaa cgctggcgat gcaaaaaccg ggctgtgaag tgatcaccgg caccaatttg
     7561 caactgctac tggagatggt gctggagcgc gaagggttaa gcggtgaaga attccgtgta
     7621 caggcgctgg agtgtggaca ccgagggctg accagtctgg tggacgagtt aggccgctgc
     7681 catgaagaat gtccggtcga ggaaggaata tgacacacgt tctgcgcgcc agaaggctgc
     7741 tgactgaaga gggatggctc gatgaccatc agttgcgtat tgctgacggt gtcatcgcag
     7801 caatcgaacc gattccagtg ggcgtgactg aacgcgatgc ggaactgctc tgccccgctt
     7861 acatcgacac ccatgtacac ggtggtgcgg gcgttgatgt aatggatgac gcgccggatg
     7921 tactcgacaa gctggcaatg cacaaggcac gcgaaggtgt cggcagttgg ttgccgacta
     7981 ccgtaaccgc gccgcttaac agcattcatg cggcgctgaa acgtattgct caacgttgcc
     8041 aacgcggcgg acctggtgcg caagtgctgg ggagttatct cgaaggaccg tacttcacgc
     8101 cgcagaataa aggcgcgcat ccgccggaat tgtttcgcga gcttgaaatt gccgagctgg
     8161 atcaattgat tgccgtttct cagcacacct tacgcgtggt agcgctggca ccggaaaaag
     8221 agggggcatt gcaggccatc cgccatctta aacagcaaaa tgtacgagtg atgctggggc
     8281 atagcgcggc gacctggcaa caaactcgcg ccgcgtttga tgctggtgcc gacggcctgg
     8341 tgcattgcta taacgggatg acaggtttac atcaccgcga accgggaatg gttggcgcgg
     8401 gattaacgga caagcgcgcc tggctggaac tgatagccga tggtcatcat gtgcatccgg
     8461 cggcgatgtc gctgtgttgt tgctgtgcaa aagagagaat cgtgctgatc accgacgcga
     8521 tgcaggcagc cgggatgccg gatggtcgct atacgttatg tggcgaagaa gtgcagatgc
     8581 acggtggcgt tgtccgtacc gcgtccggtg ggctggcggg cagtacgctg tctgttgatg
     8641 cggcagtgcg caacatggtc gagttgacgg gcgtaacgcc tgcggaagcc attcatatgg
     8701 catcgctgca tccggcgcga atgctgggtg ttgatggtgt tctgggatcg cttaaaccgg
     8761 gcaaacgcgc cagcatcgtt gcgctggata gcgggctgca tgtgcaacaa atctggattc
     8821 agagtcaatt agcttcgttt tgatattttg ctcctttatt gggccttcac ttcccccgta
     8881 aggcctttct ttttctttcg ttttgatctg tgcagcggtg tcggatgcga cgctaacgcg
     8941 tcttatgcga cctacggtac gccgcatcca gcaatcggtg aacatagcga cgtcattacg
     9001 caattcttta atcttccctt gtcttttctt tcaatgatca cagatttcgt tttatttcct
     9061 ttttctccat tgaactttcg gtttcttttc tatagatttt aatcaacgaa agacatcacc
     9121 aagtgaaatg aaacgaaagg caagtgaaag cgacaacgcc cgacgtcaag ttcatcagac
     9181 taaggattga gttatgccag aaaattacac ccctgctgcc gccgcaaccg gtacatggac
     9241 tgaagaagag atccgccatc agcctcgcgc atggatccgt tcactcacca acatcgacgc
     9301 gctacgttct gcgctcaata acttccttga accgttactg cgcaaagaga atctgcggat
     9361 catcctgacc ggagccggaa cctcggcatt tatcggtgac atcatcgcgc cgtggctcgc
     9421 cagccatacc ggtaaaaact tcagcgccgt accgaccacc gatctggtca ccaatccgat
     9481 ggactacctg aacccagctc atccgctgct gttgatctcc ttcggtcgat ccggcaacag
     9541 cccggaaagc gtcgctgccg tggaactggc aaatcaattt gtaccagaat gctatcacct
     9601 gccgatcacc tgcaacgaag cgggcgctct ttaccaaaac gcgatcaaca gcgataacgc
     9661 gtttgccctg ctgatgcccg cagaaacgca cgatcgcggc tttgcgatga ccagcagcat
     9721 taccaccatg atggccagct gcctcgcggt tttcgcacct gagacgatca acagccaaac
     9781 cttccgcgac gtggcggatc gttgccaggc gatcctgacc tcactgggcg atttcagcga
     9841 aggtgtgttt ggttacgcac cgtggaaacg gatcgtttat ctcggcagcg gtggcttaca
     9901 gggcgcagca cgcgagtcgg cgctgaaagt gctggaactg actgcgggta aactggcggc
     9961 cttctatgat tccccgaccg gattccgtca tggcccgaaa tcactggtcg ataacgaaac
    10021 gctggtggtg gtatttgtct caagccaccc ttacacccgt cagtatgatc ttgatctgct
    10081 ggctgaactc cgccgtgaca accaggcaat gcgcgtaatc gccatcgccg cggaaagcac
    10141 cgacatcgtc gctgccggtc cacatattat cctgccgccg tcacgtcact ttatcgacgt
    10201 tgagcaggca ttttgcttcc tgatgtacgc ccagacgttt gcactgatgc agtcgctgca
    10261 catgggcaat acgccggata ccccatcagc cagcggcacc gttaaccgcg tggtgcaagg
    10321 cgtaatcatt catccgtggc aggcataaga ggatcgcatt atgagcatta tctccactaa
    10381 atatctgtta caggacgccc aggccaatgg ctacgcggtg cctgctttta acattcataa
    10441 cgccgagacg atccaggcga tcctcgaagt gtgcagtgaa atgcgatcgc cggtgatcct
    10501 cgccggaacg ccggggacct ttaaacatat cgcgctggaa gagatctacg ccctgtgcag
    10561 cgcctattcc acaacctaca acatgccact ggcgctgcat ctcgaccacc acgaatcgct
    10621 ggatgatatt cgccgtaaag tccacgcagg tgtgcgcagc gcaatgatcg acggcagcca
    10681 cttcccgttt gccgagaacg tgaagctggt gaaatcggtt gttgacttct gccactcaca
    10741 ggattgcagc gtggaagcag aactgggccg cctgggcggt gttgaagatg acatgagcgt
    10801 tgacgccgaa agtgcattcc tgaccgatcc acaagaagct aaacgctttg tcgaactgac
    10861 tggcgtcgac agcctggcgg tagcgattgg tacggcgcac ggcttataca gcaaaacgcc
    10921 gaagattgat ttccagcggc tggcggaaat tcgtgaagtg gtggatgttc ctctggtgct
    10981 acatggtgcc agcgatgttc cggatgaatt tgtccgtcgc actattgaac ttggcgtcac
    11041 aaaagtgaac gttgccacag aattaaaaat agccttcgct ggcgcggtta aagcctggtt
    11101 tgcggaaaat ccgcagggta atgatcctcg ttattatatg cgcgtcggaa tggatgcgat
    11161 gaaagaagtt gtcagaaata aaattaatgt ctgtggttca gcgaatcgaa tttcagcata
    11221 atcattagtt ttttatgatt tatcccaatg tacttcccga ttaatatcgg ggagtgcctt
    11281 aatggaaaag gagatgacta aaccttaata aatacatcag tacaatatcg caacaataat
    11341 atattaaaaa aaattatatt attcaacttt atggtgagga ttacacaatg agcagtccaa
    11401 atattctctt aacccgtatt gataaccgtc tggttcacgg tcaggttggc gtgacctgga
    11461 catccaccat cggtgcgaat ctgctggtag tcgtggatga tgttgtcgct aacgatgata
    11521 ttcaacagaa attaatgggt attaccgcag aaacctacgg ctttggcatt cgtttcttta
    11581 ctatcgaaaa aaccattaac gtcatcggca aagctgcacc gcatcagaag atcttcctga
    11641 tttgccgtac gccacaaacg gtacgtaaat tggtagaagg tggcattgac ctgaaagata
    11701 tcaacgtcgg caatatgcat ttctcggaag ggaaaaagca aatcagcagt aaagtttatg
    11761 tcgatgacca ggatctcgcg gacttacgtt ttattaaaca acgtggcgtg aatgttttca
    11821 ttcaggacgt ccctggcgat caaaaagaac aaatccctga ctaaatctaa aatcgcctta
    11881 atattggttt gaggtaataa aaatgcatga aataacccta cttcagggat tatccctggc
    11941 ggcgttagtt ttcgttctgg ggattgattt ttggctggaa gccttatttt tattccgccc
    12001 gataatcgtt tgtaccctaa ctggcgctat tctcggtgat attcagactg gcttaattac
    12061 cggtggtctg acagagttgg ctttcgccgg attaacccct gcaggtggtg ttcagccgcc
    12121 caacccgatt atggcgggtc tgatgaccac cgtcattgca tggtctacgg gcgttgatgc
    12181 caaaacagca attggtcttg gcctgccgtt tagtttgtta atgcagtacg tcattctgtt
    12241 cttctattcc gctttctcat tatttatgac caaagccgat aaatgcgcga aagaggcgga
    12301 tacggcagcg ttttcccggc ttaactggac aacgatgctc atcgtcgctt cagcgtatgc
    12361 ggtgattgct ttcctctgta cttacctggc acaaggggcg atgcaggcgc tggtgaaagc
    12421 gatgcccgcc tggttgaccc acggctttga agtggctggc ggtattctgc ctgccgttgg
    12481 ttttggcttg ctgctgcgcg taatgttcaa agcgcaatat atcccttacc tgatcgccgg
    12541 tttcctgttt gtttgctaca tccaggtcag caacctgttg ccggttgccg tactgggcgc
    12601 aggctttgcg gtgtatgagt ttttcaatgc gaaatcccgg cagcaagcgc aaccgcagcc
    12661 cgttgccagt aaaaatgaag aagaggacta cagcaatggg atctgaaatc agtaaaaaag
    12721 atatcacccg tctgggcttt cgttcgtcgc tgctgcaagc gagctttaac tacgaaagga
    12781 tgcaggcggg cggttttacc tgggcgatgt tgccgatcct gaaaaagatt tataaggacg
    12841 acaaaccggg cttaagcgcg gcgatgaaag ataacctcga atttattaat acccacccga
    12901 atctggtcgg attcctgatg gggttattaa tttcgatgga agaaaaagga gaaaaccgcg
    12961 acaccattaa aggcctcaaa gtggcactgt ttggcccaat cgccgggatt ggcgatgcga
    13021 ttttctggtt tactttgttg ccgattatgg cgggaatttg ctcatcattt gccagccagg
    13081 gaaacctgct ggggccgatt ctatttttcg ccgtttacct gcttatcttt ttcctgcgcg
    13141 tcggctggac ccacgtcggt tattcagtcg gcgtgaaggc gatcgataaa gtgcgagaga
    13201 actcgcagat gattgctcgc tcggcaacca tcctcggaat cacggtaatc ggcgggctga
    13261 tcgcttcgta tgtgcatatt aacgtggtga catcgtttgc catcgacagt acccacagcg
    13321 tcgcgctgca acaggatttc ttcgataaag tcttcccgaa cattttaccg atggcctaca
    13381 ccctgctgat gtattacttc ctgcgggtga aaaaagcgca tccggtgctg ttaatcggcg
    13441 tgacttttgt gctctctatt gtttgttccg cattcggcat tttgtaaatg gaacgaggca
    13501 ctgcttctgg cggtgcctct ttacttaagg aatttcatcc tgtgcaaacc cttcagcaag
    13561 ttgaaaacta tacggcgtta agtgaacgag ccagcgaata tttattggct gtgatccgta
    13621 gcaaaccgga tgccgtgatt tgcctggcga ccggagccac gccattactg acgtatcatt
    13681 atctggtaga aaaaatccac cagcagcagg ttgatatcag ccagctcacc ttcgtgaagc
    13741 tcgacgaatg ggtggatctg ccattaacga tgcctggcac ctgcgaaact ttcctacaac
    13801 agcatatcgt gcagccgctg gggctgcgtg aagaccagct catcagcttt cgctccgaag
    13861 agataaatga gacagagtgc gaacgggtaa cgaacctgat tgcgcgcaaa ggcggtctgg
    13921 atttatgcgt tctaggattg gggaaaaacg gtcatcttgg gctgaacgaa ccgggagaaa
    13981 gcctacaacc ggcctgccat atcagtcaac ttgatgccag aacacagcaa catgagatgt
    14041 taaaaaccac gggtcgcccc gtgactcgtg ggatcacctt aggcctgaag gatattctca
    14101 atgcccgcga ggttttgtta ttggtgactg gcgaaggaaa gcaggatgcg acagaacgtt
    14161 ttctcacggc taaagtctcc accgctatcc cggcttcgtt tttatggctg cacagtaact
    14221 ttatttgttt aattaatacc taaccagatg aaaatttgct caaaattttt aaattaactt
    14281 attcaacagt cacgcattat attaaataac atttgactgg gttgaacata acgccaatag
    14341 caaaaggagt tatgttcaac aatttaattt tagatataca tataacttgt tctattaata
    14401 aatcacctcg tattctgact cggcccctgg cgcagcaaag tcaggcattt atactcttt
//
LOCUS       DAZFQD010000135        14456 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.135, whole genome shotgun sequence.
ACCESSION   DAZFQD010000135 DAZFQD010000000
VERSION     DAZFQD010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14456
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.135"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            16..582
                     /locus_tag="ACHKTW_004087"
     CDS             16..582
                     /locus_tag="ACHKTW_004087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO6465010.1"
                     /translation="MFKGQKTLAALAVSLLFTAPVYAADEGSGEIHFKGEVIEAPCEI
                     HQDDIDKEVELGQVTTSHINQSHHSDAVAVDLRLVNCDLENSSNGSGGKISKVAVTFD
                     SSAKTTGADPILNNTSTGEATGVGVRLMNKDQSNIVLGTATPDIDLAPTSSEQTLNFF
                     AWMEQIDQATPVTPGAVTANATYVLDYK"
     gene            633..3092
                     /locus_tag="ACHKTW_004088"
     CDS             633..3092
                     /locus_tag="ACHKTW_004088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFO6465011.1"
                     /translation="MDTVNIYRLSFVSCLVVAMPCALAVEFNLNVLDKSMRDRIDISL
                     LKEKGVIAPGEYFVSVAVNNNQISNGQKINWHKNDDKTIPCINDLLVDKFGLKPEVRQ
                     SLPLINQCVDFSSRPEMLFNFDQANQQLNITIPQAWLAWHSENWTPPSTWKEGVAGVL
                     MDYNLFASSYRPQDGSSSTNLNAYGTAGINTGAWRLRSDYQLNQTDSDDNHEQSGEIS
                     RTYLFRPLPQLGSKLTLGETDFSSNIFDGFSYTGAALASDDRMLPWELRGYAPQISGI
                     AQTNATVTISQSGRVIYQKKVPPGPFIIDDLNQSVQGTLDVKVTEEDGRVNNFQVSAA
                     STPFLTRQGQVRYKLAAGQPRPSMSHQTENETFFSNEVSWGILSNTSLYGGLLLSGDD
                     YHSAAIGIGQNMLWLGALSFDVTWASSHFDTQQDERGLSYRFNYSKQVDATNSTISLA
                     AYRFSDRHFHSYANYLDHKYNDSDAQDEKQTISLSVGQPITPLNLNLYANLLHQTWWN
                     ADASTTANITAGFNVDIGDWRDISISTSFNTTHYEDKDRDNQIYLSISLPFGNGGRVG
                     YDMQNSSHSTTHRMSWNDTLDERNSWGMSAGLQSDRPDNGAQVSGNYQHLSSAGEWDI
                     SGTYAANDYSSVSSSWSGSFTATQYGAAFHRRSSTNEPRLMVSTDGVADIPVQGNLDY
                     TNHFGIAVVPLISSYQPSTVAVNMNDLPDGVTVAENVIKETWIEGAIGYKSLASRSGK
                     DVNVIIRNASGQFPPLGADIRQDDSGISVGMVGEEGHAWLSGVAENQKFTVVWGDSQH
                     CSLHLPEHMEDTANRLILPCH"
     gene            3107..3838
                     /locus_tag="ACHKTW_004089"
     CDS             3107..3838
                     /locus_tag="ACHKTW_004089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFO6465012.1"
                     /translation="MTFMKGLPLLLLVASLCSHAALQPDRTRIVFNANDKATSLRVDN
                     RSDKLPYLAYSWLENEKGEKSDDLLVALPPIQRLEPKATTQVRIVKQASTTKLPGDRE
                     TLFFYNMREIPPAPEKNSDHAVLQVAIQSRIKVFWRPAALRKKAGEKVELQLQVSQQG
                     NQLTLKNPTAYYLTIAYLGRNEKGVLPGFKTVMVAPFSTVNTNTGNYSGSQFYLGYMD
                     DYGALRMTTLNCSGQCHLQAVEAKK"
     gene            3835..4896
                     /locus_tag="ACHKTW_004090"
     CDS             3835..4896
                     /locus_tag="ACHKTW_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO6465013.1"
                     /translation="MSAGKGVLLVICLLFLPLKSALALNCYFGSSGGSVEKSEAIQPF
                     AVPGNAKLGDKIWESDDIKIPVYCDNNTNGNFESEHVYAWVNPYPGVQDRYYQLGVTY
                     NGVDYDANQGKSRIDTNQCIDSKNIDIYTPEQIIAMGWQNKICSGDPANIHMSRTFLA
                     RMRLYVKIREMPPHDYQSTLSDYIVVQFDGAGSVNEDPTAQNLKYHITGLENIRVLDC
                     SVNFSISPETQVIDFGKFNLLDIRRHTMSKTFSIKTTKSQNDQCTDGFKVSSSFYTEE
                     TLVEEDKALLIGNGLKLRLLDENASPYTFNKYAEYADFTSDMLVYEKTYTAELSSIAG
                     TPIEAGPFDTVVLFKINYN"
     gene            5048..6094
                     /locus_tag="ACHKTW_004091"
     CDS             5048..6094
                     /locus_tag="ACHKTW_004091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFO6465014.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLAVHLPAALLLGPMIAGIIFSMRG
                     ITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAANWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCLLRFPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(6091..6882)
                     /gene="nei"
                     /locus_tag="ACHKTW_004092"
     CDS             complement(6091..6882)
                     /gene="nei"
                     /locus_tag="ACHKTW_004092"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000703 - oxidized pyrimidine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HFO6465015.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVETRGKALLTHFSNDLTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLRPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLDIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGELCERCGGIIEKTTLSSRPFYWCPGCQH"
     gene            complement(6918..7652)
                     /gene="pxpA"
                     /locus_tag="ACHKTW_004093"
     CDS             complement(6918..7652)
                     /gene="pxpA"
                     /locus_tag="ACHKTW_004093"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HFO6465016.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQTMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALVLVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQLGALIENEEQALAQTLEMVQHGRVKSITGEWATVTAQTVCLH
                     GDGEHALAFARRLRSTFAEKEIVVAA"
     gene            complement(7642..8574)
                     /gene="pxpC"
                     /locus_tag="ACHKTW_004094"
     CDS             complement(7642..8574)
                     /gene="pxpC"
                     /locus_tag="ACHKTW_004094"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
                     /protein_id="HFO6465017.1"
                     /translation="MLKIIRAGMYTTVQDGGRHGFRQSGISHCGALDMPALRIANLLV
                     GNDANAPALEITLGQLTVEFETDGWFALTGAGCEARLDDNAVWTGWRLPMKAGQRLTL
                     KRPQHGMRSYLAVAGGIDVPPVMGSCSTDLNVGIGGLEGRLLKDGDRLPIGKSKRDFM
                     EAQGVKQLLWGNRIRALPGPEYHEFDRASQDAFWRSPWQLSPQSNRMGYRLQGQILKR
                     TTDRELLSHGLLPGVVQVPHNGQPIVLMNDAQTTGGYPRIACIIEADMYHLAQIPLGQ
                     PIHFVQCSLEEALKARQDQQRYFEQLAWRLHNEN"
     gene            complement(8568..9224)
                     /gene="pxpB"
                     /locus_tag="ACHKTW_004095"
     CDS             complement(8568..9224)
                     /gene="pxpB"
                     /locus_tag="ACHKTW_004095"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HFO6465018.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAHCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGVC
                     "
     gene            complement(9247..9990)
                     /gene="ybgI"
                     /locus_tag="ACHKTW_004096"
     CDS             complement(9247..9990)
                     /gene="ybgI"
                     /locus_tag="ACHKTW_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HFO6465019.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGLELASWIEARLGRKPLWCGDT
                     GPEVVLRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            10261..11742
                     /gene="dtpD"
                     /locus_tag="ACHKTW_004097"
     CDS             10261..11742
                     /gene="dtpD"
                     /locus_tag="ACHKTW_004097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HFO6465020.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSSMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
     gene            complement(11784..13202)
                     /gene="phrB"
                     /locus_tag="ACHKTW_004098"
     CDS             complement(11784..13202)
                     /gene="phrB"
                     /locus_tag="ACHKTW_004098"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HFO6465021.1"
                     /translation="MITHLVWFRQDLRLHDNLALAAACRNSSARVLALYIATPRQWAT
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFVASVEIVKQVCAENSVTHLFY
                     NYQYEVNERARDVEVERALRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIEPAPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCQN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGAGSVWLSEL
                     IWREFYRHLMTYYPSLCKHCPFIAWTDRVQWQSNPAHLQAWQKGKTGYPIVDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPITQGEKFDREGEFIRRWLPELRDVPGKAVHEPWKWAQKAGVMLDYPQP
                     IVDHKEARLRTLAAYEEARKGA"
     gene            complement(13199..13708)
                     /locus_tag="ACHKTW_004099"
     CDS             complement(13199..13708)
                     /locus_tag="ACHKTW_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HFO6465022.1"
                     /translation="MNQQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDFYNQYYQRVIVLLSHPANPRDHTNVLMHVQGY
                     LRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYPDAWLSGQRYFELWPRV
                     INLRHSGVL"
     gene            complement(13859..14456)
                     /locus_tag="ACHKTW_004100"
                     /pseudo
     CDS             complement(13859..14456)
                     /locus_tag="ACHKTW_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
BASE COUNT         3496 a         3859 c         3503 g         3598 t
ORIGIN      
        1 ttaaaggact ccgttatgtt taaaggacaa aaaacattgg ccgcactggc cgtatctctg
       61 ctgttcacag cacctgttta tgcggctgat gaaggatccg gtgaaattca ctttaaaggt
      121 gaagttattg aagcaccgtg tgaaatacat caggatgata ttgataaaga ggttgaactc
      181 ggtcaggtga ccaccagcca cattaatcag tcacatcaca gcgatgccgt tgctgtcgac
      241 ctgcgcttag tcaactgtga tctggaaaac tccagcaacg gttccggtgg caagatttct
      301 aaagttgcag tcacctttga tagctcagcg aaaaccaccg gcgcagatcc gattctcaac
      361 aacaccagca cgggtgaagc caccggcgtc ggcgtacgtt taatgaataa agatcaaagc
      421 aatatcgttt taggcaccgc tactccagat atcgacctgg ctccgacctc cagcgaacaa
      481 acgctgaatt tctttgcctg gatggaacaa attgatcagg ctacacctgt aacgccaggc
      541 gcagttacgg cgaatgcaac ctacgtgctg gattataaat aagtattact ttggcggcgc
      601 tatcgccgcc tttcataaaa tatttatcag gtatggacac cgtgaatatt tatcgactct
      661 cttttgtatc ctgcctggtc gtggcgatgc cttgcgcatt ggcggtcgaa ttcaacctta
      721 atgttctcga taaatcaatg cgcgaccgca ttgatatttc attattaaag gaaaaaggag
      781 tcattgctcc cggtgagtat tttgttagcg ttgcggtaaa taataaccaa atcagtaacg
      841 ggcaaaagat taactggcac aaaaatgacg ataaaaccat tccgtgcatc aatgatttac
      901 tggtcgataa atttggctta aaacctgaag tccgtcagtc gttaccattg ataaatcagt
      961 gcgtcgattt tagctcccga cctgaaatgc tcttcaattt cgatcaagcc aatcagcaac
     1021 taaatatcac cattccgcaa gcctggctgg cgtggcactc agaaaactgg accccaccct
     1081 ccacatggaa agaaggtgtc gccggtgtcc tgatggatta caacttgttt gccagcagct
     1141 accgcccaca ggatggcagc agcagcacta atcttaacgc ctatggaaca gccggaatca
     1201 acacgggggc atggcgctta cgcagtgatt atcagttgaa tcagactgat agcgatgata
     1261 accatgaaca gtcaggcgaa atatcacgta cctatctttt tcgcccgtta ccgcaattag
     1321 gttcaaaatt aaccctcggc gaaacggatt ttagttccaa tattttcgac ggtttttctt
     1381 ataccggcgc ggcactggca agtgacgatc gaatgttgcc atgggaacta cgcggctacg
     1441 ccccacaaat tagcggtatt gcacagacca atgccacggt gacgatcagt caatcaggcc
     1501 gcgtcattta ccagaaaaaa gtcccaccag gcccatttat cattgacgac cttaatcagt
     1561 ctgttcaggg cacactggat gtcaaagtga cggaagaaga tggtcgggtg aacaatttcc
     1621 aggtttcggc agcatcgacg cccttcctga ctcgtcaggg acaggttcgc tataaactgg
     1681 ccgcgggtca gccacggccc tccatgtcac atcaaactga aaatgaaacc ttttttagca
     1741 atgaagtttc ctgggggata ctgtcaaaca cctcgctgta cggcggcctg ctgctttctg
     1801 gtgatgacta ccattctgcc gcaataggta tcgggcaaaa tatgctgtgg cttggcgcgc
     1861 tgtcgtttga tgtcacgtgg gccagtagtc actttgatac tcagcaggac gagcggggct
     1921 taagctaccg ttttaattac agcaaacaag tggatgccac taacagcacg atttccctcg
     1981 ccgcttatcg tttctccgat cgtcattttc acagctacgc caactatctg gatcacaaat
     2041 acaacgacag cgatgcgcag gacgaaaaac agacgatcag cttatctgtg ggccaaccga
     2101 ttaccccact aaacctcaat ctttacgcca acctgctaca tcaaacctgg tggaatgcag
     2161 acgcctccac gaccgccaac atcacggcag gttttaatgt tgatattggt gactggagag
     2221 atatctctat ttctacgtca ttcaatacaa cccattacga agataaagat cgcgacaacc
     2281 agatttacct gtcgatttcg ctccccttcg gtaacggtgg tcgggttggc tatgacatgc
     2341 aaaacagtag ccacagcacc acacaccgca tgtcgtggaa cgatacgctg gatgaacgta
     2401 atagctgggg catgtctgcc ggactgcaat ccgaccgtcc tgacaatgga gcccaggtga
     2461 gcggtaacta tcagcacctg agttcagcgg gtgagtggga tatttctggt acctatgccg
     2521 ccaatgatta cagttccgtc agcagcagct ggagcggttc tttcaccgca acccaatatg
     2581 gtgcagcgtt tcatcgccgc agctccacca atgaaccacg cctgatggtc agcaccgatg
     2641 gcgtggcaga tattccggtt cagggcaatc tcgactacac caaccatttt ggcattgcgg
     2701 tggtgccgtt gatttccagt taccagcctt ccaccgtggc ggtgaacatg aatgacttac
     2761 ccgacggcgt aacagttgca gaaaacgtta tcaaagaaac gtggattgaa ggcgcgatag
     2821 gttacaaatc actggcttcc cgttccggta aagacgttaa cgtcatcatt cgcaacgcca
     2881 gcggtcagtt tcctcccctc ggagcggata tccgccagga tgacagcggc attagcgtgg
     2941 ggatggttgg cgaggaagga catgcctggt taagcggagt cgctgaaaat caaaagttta
     3001 ccgtggtctg gggtgatagc cagcattgct cgctccatct tcctgaacat atggaagaca
     3061 ccgcaaatcg cctgatttta ccttgtcatt aattaaggaa atgaaaatga catttatgaa
     3121 aggactaccc cttttgttgt tggttgccag tctgtgcagc cacgctgcac tacaacccga
     3181 tcgcactcgt attgtgttta acgctaatga taaagctacc agtctgcggg tggataatcg
     3241 tagcgataaa ctcccctacc tcgcttattc ctggctcgaa aatgaaaaag gggagaaaag
     3301 cgatgatctt ctggtcgccc taccgccgat acaacgtctg gaaccgaaag caacaacgca
     3361 ggtacggata gtaaagcaag cctcgaccac aaaattaccg ggcgatcgcg aaacgctatt
     3421 tttctacaat atgcgtgaaa ttcctcccgc cccggaaaaa aacagcgacc atgccgttct
     3481 gcaagtcgcc attcagagtc gtatcaaggt gttctggcga ccggctgcat tacgcaagaa
     3541 agcaggagaa aaggtcgaac tgcagttaca ggtcagccag cagggcaacc aactgacact
     3601 gaaaaatcct accgcgtatt atctgaccat tgcctacctg ggacgaaatg aaaaaggcgt
     3661 tctccccggc ttcaaaaccg tgatggttgc gccttttagt acggtcaaca caaacaccgg
     3721 aaattatagc ggcagccagt tttatctcgg ttatatggat gattacggcg cgttacgcat
     3781 gacgacgctc aactgcagcg gacaatgcca tttacaagcc gtggaggcga agaaatgagt
     3841 gctggcaagg gagtgttgct cgtcatctgt ctgttatttc tgccgttgaa gtctgcgctt
     3901 gccctgaact gctactttgg ttcatccggt ggctcggtag aaaaatcaga agcgattcaa
     3961 ccttttgctg taccaggcaa tgccaaactc ggcgataaga tttgggaatc tgacgatatt
     4021 aaaattcccg tctattgtga caacaatacc aacggcaatt ttgaaagtga gcatgtttat
     4081 gcctgggtaa acccctaccc gggggtgcag gatcgctatt atcaactcgg cgtaacttac
     4141 aatggcgttg attatgacgc caaccaggga aaaagccgca tcgacactaa ccagtgtatt
     4201 gacagtaaga acattgacat ttatacccct gagcagatta tcgcgatggg atggcagaac
     4261 aaaatttgct cgggtgatcc cgctaatatt cacatgtcgc gtacctttct cgctcgcatg
     4321 cggttatacg tcaaaatacg agaaatgccg ccgcatgatt atcaaagtac gcttagcgac
     4381 tatatcgtcg tgcaatttga cggtgccggt agcgttaacg aagaccctac tgcccaaaac
     4441 ctgaaatatc atattactgg tctggaaaac attcgcgtgc tggattgcag cgtcaatttt
     4501 tccatttcac cggaaacaca ggtgattgat tttggtaaat ttaatttgct ggatattcgt
     4561 cgccacacaa tgtcgaaaac attcagtatt aagacgacaa aatcacaaaa tgaccaatgc
     4621 accgacggat ttaaagtcag ttcctccttt tataccgaag aaacgttggt tgaagaagat
     4681 aaagcattgt tgattggtaa tggtctgaag ctacgtttgc tggatgaaaa cgcctcgcct
     4741 tataccttca ataaatatgc tgaatacgcc gatttcacca gcgacatgtt ggtctacgaa
     4801 aaaacctata cagctgaact ttcgtccatt gcaggtaccc ccatcgaggc tgggcccttc
     4861 gatacagtgg tgctttttaa aattaactat aactgatttg ccatgatgcc ctttgcagcc
     4921 ggaaagggca tctctttcag aaaattgctg attttcccct tttccttgat tgctgttatt
     4981 ttttcctggc ccggctttct ttatgctgta tcgatacacc aaagagaaaa taatgaggga
     5041 gcgaaggatg ccagttttgc agtggggaat gttatgtgtg ctatcacttc tcctttctat
     5101 tggtttcctc gcggtacatc tcccggcggc gctattactt gggccgatga tcgccgggat
     5161 catcttcagt atgcgcggaa ttactctgca acttccccgc tccgcttttc tcgccgcgca
     5221 ggccattctt ggctgcatga ttgcgcaaaa cctcactggt tcgattctca ccacgctggc
     5281 cgccaactgg ccgatcgtgc ttgctatttt actggtgacg ctgctttcca gcgccatcgt
     5341 gggttggtta ttggtgcgct atagctcatt gcctggaaat accggtgcct ggggttcttc
     5401 tcctggcggc gcggcggcaa tggtcgctat ggcgcaagat tacggcgcag atattcgcct
     5461 ggttgcattt atgcaatatc tgcgagtgct gttcgtcgcg ggggccgctg ttttggtcac
     5521 ccgcatgatg ctgggtgata acgctgaagc ggttaaccag caaatcgtct ggttcccgcc
     5581 agtgagcatt aatctcctgc ttacgatttt gctcgcggtc gttgccggta cggcgggatg
     5641 cttgctacgt tttccttccg gcacgatgct catcccgatg ctggcgggcg cagtgctcca
     5701 gtctggtcag ctcatcacca tcgaactccc ggaatggctg cttgcgatgg cgtatatggc
     5761 aattggctgg cggattggtc ttggtttcga taaacaaatc ttactgcggg cactacgccc
     5821 gctaccgcaa atcctgctgt cgatttttgc tctgctggct atttgtgcgg gtatggcgtg
     5881 ggggctgacc cggtttatgc acattgattt tatgactgcc tacctcgcca ccagtcccgg
     5941 cgggcttgat accgtggcgg tcatcgccgc agggagcaat gccgatatgg cgcttattat
     6001 ggcgatgcaa accctgcgcc tgttcagtat tttgctgacg gggccagcca ttgcgcggtt
     6061 tatttcaacc tatgcgccga agcggtcggc ctagtgctgg cagcctggac accagtaaaa
     6121 cgggcgcgag gacaacgtgg ttttctcaat gatgccgcca cagcgttcgc acagttcgcc
     6181 atcacgatga aaaaccttaa agcgaaacag cgccccatga tgcttattct catccacctg
     6241 cccccgcgta gcgtaagaaa accgaggaat atccagtaac gcgtgtgcga gtgcatccag
     6301 ttgcgccgca ttgaggtctt tcgctttatg atttccagtc aacccaacct gccagaggat
     6361 ctccacccgc aaataattgc caagtcctgc cagaaacgcc tgatcgagca gtaatccagc
     6421 aaactgacgg ttacgaaagc gcggtgacaa taaccgttcc tttaccacct ccggcgtcag
     6481 attcggatcc agcacatcgg gaccaacgcg ttgtaaaaac ggatgcgtgg tcagttgttc
     6541 cggcctcaac atctcaatat ccgaggcgct ataaagcaga atggttttgt cagccgtttg
     6601 cagttttacc cgcaatactc gcgtggtctg cggctcttcg ccggtatcaa ccacgcgcca
     6661 gacgccgtaa agctgattat ggctgtagag cgttaagtcg ttggaaaaat gagttaacaa
     6721 cgccttacca cgcgtttcca catgggtaac gtgttgaccg ataagttgtg attgataagt
     6781 ttttaactgc gggaaggcaa accagacatc agttagtggt ttgcctttga tcgccgcctc
     6841 caggttatcc gctgcacggc ggatctccgg gccttcaggc atggtgatat ccttttttca
     6901 ttctttatta atcggggtta tgctgcgaca acaatctcct tttcggcaaa tgtagagcgt
     6961 agtcggcggg cgaacgccag tgcgtgctcg ccgtcgccat gcaggcagac tgtttgcgcc
     7021 gtgaccgttg cccattcgcc ggtgatactt ttgactctgc cgtgttgcac catctctagc
     7081 gtttgcgcca gcgcctgttc ttcgttttca atcaacgcgc ctagctgact tcgcggcacc
     7141 agcgagccgt cagcctgata accgcgatcg gcaaacactt cctcgcgcgt tgtcaggcca
     7201 tattgcttgc ctgcacgaat cagctcgctt cctgccagcc caacgagaac caatgctgga
     7261 tcgcaagcgt ataccgctct ggcgatggcg tctgccagtt gtgcttcttt cgccgcctgg
     7321 ttgtacaaca tgccgtgcgg tttgacatga cgcattacgc cgccttgcgc acgggcaatc
     7381 gttgccagcg cgccaatttg atacagcgtc tgggcgtaaa cggtttctgg cggcagctgc
     7441 atggcgctgc gaccaaaatt ttccctgtcg ggaaaactcg ggtgagcgcc aatcgcgaca
     7501 ccatttttta ttgcttcacg cacgcaagcc tgcatggttt gggcatcgcc tgcatgaaat
     7561 ccacaggcaa tattggcaga ggaaaccagc gttaatagct ctgcgtcgct ggcgcagcct
     7621 tcgcccagat cggcgttcag gtcaattttc attgtgtagc cgccacgcta attgttcgaa
     7681 ataacgttgt tgatcttgcc gcgctttcag tgcctcttcc agtgaacact ggacaaaatg
     7741 aatcggctga ccgagcggaa tttgcgccag atggtacata tcagcctcaa tgatacaggc
     7801 aatacgcggg taaccaccgg tggtctgtgc gtcgttcatc aacacaatag gctgcccgtt
     7861 atgcggcacc tgcaccacac ccggtaacaa accgtgagat aacagttcgc gatcggtggt
     7921 gcgttttaaa atttgcccct gtaagcgata gcccatgcgg ttactttgcg ggctaagctg
     7981 ccagggcgaa cgccagaatg catcctgcga ggcgcgatcg aactcatgat attccggccc
     8041 cggcaaggcg cgaatgcggt tgccccacag cagctgttta acgccctgcg cttccataaa
     8101 atcacgcttc gatttgccaa tcgggagtcg gtcaccatcc ttcagtaaac ggccttccag
     8161 cccgccaatc cccacgttga gatcggtgct gcatgacccc attaccggcg gaacatcaat
     8221 accacccgcg accgccagat aactgcgcat cccgtgctgc gggcgtttaa gcgttaaacg
     8281 ctggcctgct ttcatcggca atcgccagcc ggtccagacg gcattatcat ccagccgcgc
     8341 ttcgcaaccg gcccctgtca gagcaaacca cccatcagtt tcgaactcaa ccgttaactg
     8401 accgagcgtg atctccagcg cgggggcatt ggcgtcatta cccaccagca ggttagcaat
     8461 gcgcagcgca ggcatatcca gtgcgccgca gtggctgata cccgactggc gaaaaccgtg
     8521 acgaccgcca tcctgcacag tggtatacat gcccgcacga ataatcttca gcatactccc
     8581 tccttctgcg gtacaaagcg cacgctgtct cccggacgta ataagatggg ttcgtcacgc
     8641 gccggatcaa acaggctgag tgaggtatga ccaatcaact gccagccacc cggcgttgcc
     8701 agcggataaa caccagtctg cggcccgccg atcccgacag aacctgccgg aacgagcaag
     8761 cgcggttcag cgcgccgtgg cgtgtgtagt tgttccggca aactcccgag atacgggaag
     8821 cccggttgaa aacctaaaaa ccagaccacg tattccacgg aggagtgcaa ttcaacaacc
     8881 tgtttttcgc tcaacccgca atgcgccgcg accaccgcca aatccggtcc gcctgcacca
     8941 ccgtaaacca ccggaatttc aataaagcga gactcaggtt ccagggcctc gctctcctcc
     9001 caccagcgtt gcaaacgctc tatggcatcc agcgccagcg actcaggatt acgcaaaatc
     9061 accgtgatat tgttcatgcc gggaatggct tcaaccacat tcggcatatc caccagacgc
     9121 tgcgccagtc gccagatccg tttctggcta gccagcgtca ccggcggttc cagttccagc
     9181 actaccgccg tttcacctat cagataacaa cgcgctcgtt gcactttcga tccctctgat
     9241 tattcgttat gcaggattag gaatatcaat aaaggtcaca tcaagatcgg tattttcatt
     9301 cagccactcg ctcaatgcgc gaataccacc acgttcagtg gcatggtgac ctgcagcata
     9361 aaaatgcaat ccctgctcgc gggctgaatg aatggtctgt tcagaaactt cgccagtaat
     9421 aaaagcatcc acgccaaaac gcgcggcgct atcgataaaa ctttgcccgc cgcccgtgca
     9481 ccaggcgacg cgcaggacca cctcaggtcc ggtatcgcca caccataacg gcttacgtcc
     9541 cagacgcgct tcaatccagg aggctagttc cagtcccggc acaggcatgg tcagctctcc
     9601 ccacggcacc aacggctcaa tttcgcccat gaccgtgatc cccagtaacg ccgccagctg
     9661 tgcgttattg cccagctcag gatgtgcgtc aagcggcaaa tgccagccat acaggttgat
     9721 atcattcgcc agcaacgttt ttaaacggtt acgcttcatt ccgcgaatga ccggagactc
     9781 tcctttccag aagtagccat gatgcacaat gaccgcatcc gcgcccagac gcactgcctc
     9841 atcgagcaaa gcctggctgg cggtgacacc ggtaacaatt ttttgcaccg tctctttgcc
     9901 ttccacctgc aaaccattcg gcgcgtaatc actaatcgcc gcgctgttca gtttttcgtt
     9961 aatcagttgt tccagttcgg tgtttttcat cttcgcgcct tccgtattct gatagctaca
    10021 ccgttaacat agcgttacct cgccagttcg tcgatagcca atcttttgac atattaagaa
    10081 acaaacccga cgccaaaaga cgcaaattgt ttatatcatc acgcttcagc taatgtatat
    10141 ttatgcaatc agacagcata ttgcataaat aatcagatct gtatcaaagt tttaacttta
    10201 aggaaacaca agcacgccgc gcaactatta cgtgacgttg ccgtcaattg tggaattatt
    10261 atgaataaac acgcatcaca gccgcgcgct atttactatg tcgttgcgct gcaaatctgg
    10321 gaatatttta gcttttacgg catgcgtgcc ctgctgattc tctatctcac caatcaacta
    10381 aaatacaacg atactcacgc ctacgagtta tttagcgcct actgttcgct ggtgtatgtc
    10441 acgccaatcc tcggtggctt tttggcggat aaagttctcg gcaatcgcat ggcggtgatg
    10501 ctgggggcgt tattgatggc gatcggtcat gtggtgttgg gtgccagtga gatccatccg
    10561 tcattcctct atctgtccct ggcgataatc gtttgtggct atggtctgtt taaatccaat
    10621 gtcagttgcc tgctcggcga gctgtatgag ccaaccgatc cgcgtcgtga tggcggtttc
    10681 tcgctgatgt atgcagcggg taacgtgggg tctattatcg cacctattgc ctgtggctac
    10741 gcccaggaag agtacagctg ggcgatgggc tttggcctgg cggcggtggg tatgatcgcg
    10801 ggtctggtca ttttcttatg tggcaatcgt catttcacac atacccgcgg cgttaataaa
    10861 aaagtactgc gtgcgacaaa ctttctcctg cctaactggg gatggctgct ggttctgctg
    10921 gtggcaacgc ctgcgctgat taccgtattg ttctggaaag agtggtcggt atacgcctta
    10981 attgtcgcga ctattattgg cctgggtgta ctggcaaaaa tttatcgcaa agcagaaaac
    11041 cagaaacagc gtaaggagct ggggctgatt gtgacgctca ccttcttcag tatgttgttc
    11101 tgggccttcg cacaacaggg cggcagctcg attagccttt atatcgaccg cttcgttaac
    11161 cgcgatatgt ttggttatac cgttccgact gctatgttcc agtcgattaa tgccttcgca
    11221 gttatgctgt gcggtgtgtt cctggcgtgg gtggttaaag aaagcgtcgc aggtaatcgt
    11281 accgtgcgca tctggggaaa atttgctctt ggtctcggcc tgatgagcgc cgggttctgc
    11341 attctgacct taagcgcccg ctggtcctca atgtatggtc actcttctct gccactgatg
    11401 gtattaggcc tggcggtgat gggctttgcg gaactgttta tcgacccggt tgccatgtcg
    11461 caaattacgc gcattgaaat ccccggtgtg accggcgtat taaccggcat ttatatgctg
    11521 ctttccggcg cgattgcgaa ctatctcgcg ggcgtgattg ccgatcagac atcgcaggct
    11581 tcgtttgatg cttccggggc gatcaactac tccatcaatg catatattga agtatttgat
    11641 caaattacct ggggcgcact ggcgtgtgta ggactggtac tgatgatttg gctgtatcag
    11701 gcgctgaaat tcagaaaccg cgcgctggcg ctggaatctt aataggccag caccatacct
    11761 gataaaacgc tcgctgcgtt ttatcaggct cctttcctcg cttcttcgta cgctgccagc
    11821 gttcgcagcc ttgcctcttt gtgatcaact atcggttgcg gataatccag catcacacct
    11881 gctttctgcg cccacttcca cggctcatgc accgctttcc ctggtacatc gcgcagctcc
    11941 ggtaaccatc ggcggataaa ctcgccctca cggtcaaatt tctcgccctg agttatcgga
    12001 ttgaaaatac gaaaatacgg cgcagcatca gtacccgtag aagccgccca ctgccagcca
    12061 ccgttattag ctgccaaatc accatcaatc agctgcgaca tgaaatatcg ctcgccttcg
    12121 cgccagtcga tcaacaaatc tttcaccaga aaactggctg taatcatccg tagccggtta
    12181 tgcatccagc cagtactgtt aagctgacgc atagcggcat caacaatcgg gtatcccgtt
    12241 ttgcctttct gccaggcctg taaatgtgcg ggattgcttt gccactgcac acgatccgtc
    12301 caggcaataa acggacaatg tttacacaac gaggggtaat acgtcatcag atgacggtag
    12361 aactcgcgcc agatcagctc actaagccag acactaccgg ccccaccgtc cagcgcctgc
    12421 ggctgttccg ccaacaagcg atgcaaacac tggcgaggcg ataacccgcc cgttgccagg
    12481 ctggcggaca aacgactggt gccttccact gccggaaaat ctcgttgttg ctcatattct
    12541 ccggcaccgt tctggcaaaa ctggcgtaat tgcgcaatcg ccgctttttc ttccaccggg
    12601 aaatgcgcag tatcgaaaga ctgacgagga taattcagcg taatggatgg cgcgggctct
    12661 atcgatccgc tactacgaac ttttggcgca gcgacacact ccggcatccc ttcccgcagc
    12721 cgtttcagcc aggcattctt aaaaggcgta aagactttgt acatctcgtg attaccggtc
    12781 atcaccgcgc caggcggcag gatcacgctg tcatcaaatc cttcacacac cacgttacgc
    12841 agcgctcttt caacttccac atcccgcgcc cgctcattca cttcatactg atagttataa
    12901 aacaggtggg taacgctgtt ttccgcgcac acctgtttaa ctatttcgac actggcgaca
    12961 aagtcatcca cttcacggaa caataaagga ataccttttt ccgcaagcgc tatttgtagc
    13021 ccattcagtt gagcattgat aagttcagcc tgacgcggcg acatgttatg cgtcgcccac
    13081 tggcgtggtg tagcgatata taacgccagc acgcgtgcag acgaattgcg gcaggcggca
    13141 gccagtgcga gattatcgtg cagacgtaaa tcctggcgaa accagaccag atgggtaatc
    13201 ataaaactcc tgaatggcgc aagttaatca cacgcggcca aagttcgaaa taacgctgcc
    13261 ctgaaagcca ggcgtcagga taaagcgcca tatagtgttt gatacgcatc agcggcgcaa
    13321 gaagtggttg ctcgccacgg cgataactgt cgataagcgc cgccaactgc tggcgttctg
    13381 tggaatcaat atgcgggcgc aaataaccct gaacgtgcat caaaacgttg gtgtgatcgc
    13441 gcgggttggc gggatgagaa agcaaaacaa ttacgcgttg gtagtactgg ttgtaaaaat
    13501 catccagatt ttgccactgg tgaatatcag ccacgaacgg gccaagtttg cggtactccg
    13561 gctgagaatg cgccagaaag acgagtttat agcggctgtg ataatcgaga agcgccccgc
    13621 gcgttaagcc atgttctttc agtcgatgta actcatgaag ggcgaagata cggattaggg
    13681 tgctgtcatc aaatcgttgc tgattcatta ctgctcctga acggtttaca ggagtaagtg
    13741 tagacgttga gaaatgatat ttgtgaagtt aatcctgttg tagcaggaca aatttacgaa
    13801 agcctgctcc ccgcaaggac tgacgtcagg tagtttctaa ccatagctgc cctttctatt
    13861 caaacgtgaa atcaataagc tcatcaggga catcgcaaac aatatgaaga cgggtttctt
    13921 ctctgccgtg actcttttca ctaattgcgt aactgtcatg cttgggatta tttaattctt
    13981 tcagcggaaa tttttcctcg aaggctttat ttagccgccc ctggtttcct tttacagcga
    14041 ataaataatc acctccctgt ttttgtatct tctctgcaat atctttctgg caacccatcg
    14101 catcggttgt gatgattttt cctttaatat ccagcatgtt aagaagttca gggatagctg
    14161 tgatctcatt ggatttctta tccgtcttga tctgcccgat gaccagactg tgcattgttg
    14221 agaacgcact aatgacatga atcgctcccc tgcggcgact cttgtcataa gagtgccgga
    14281 gcgtttttcc atcaattgca atgacgtctt tatcatttga tgaatggcaa tcacgcatcc
    14341 agttaataaa gcactcgtga aattttgcag gactgataca ggatacaact ctggcaatag
    14401 tatcgtgaac aggaatacca ttttcaaaat caccatattg cttcaagaaa tcgaga
//
LOCUS       DAZFQD010000136        14362 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.136, whole genome shotgun sequence.
ACCESSION   DAZFQD010000136 DAZFQD010000000
VERSION     DAZFQD010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14362
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.136"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..444
                     /locus_tag="ACHKTW_004101"
     CDS             <1..444
                     /locus_tag="ACHKTW_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000023738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO6465023.1"
                     /translation="VAVLCLSTGARWGEAAGLRGEHIVGNRVMFFNTKNGKSRAVPVS
                     DTVLSLIKTRKTGLLYQADYIRFRDILQEVKPDLPKGQATHVMRHTFATHFMMNGGNI
                     VTLQRILGHATIQQTMTYAHFSPDFLQDAISFNPLADSVHKLSID"
     gene            744..3188
                     /gene="dmsA"
                     /locus_tag="ACHKTW_004102"
     CDS             744..3188
                     /gene="dmsA"
                     /locus_tag="ACHKTW_004102"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009389 - dimethyl sulfoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
                     /protein_id="HFO6465024.1"
                     /translation="MKTKIPDAVLAAEVSRRGLVKTTAIGGLAMASSALTLPFSRIAH
                     AVDSAIPTKSDEKVIWSACTVNCGSRCPLRMHVVDGEIKYVETDNTGDDNYDGLHQVR
                     ACLRGRSMRRRVYNPDRLKYPMKRVGARGEGKFERISWEEAYDIIATNMQRLIKEYGN
                     ESIYLNYGTGTLGGTMTRSWPPGNTLVARLMNCCGGYLNHYGDYSSAQIAEGLNYTYG
                     GWADGNSPSDIENSKLVVLFGNNPGETRMSGGGVTYYLEQARQKSNARMIIIDPRYTD
                     TGAGREDEWIPIRPGTDAALVNGLAYVMITENLVDQAFLDKYCVGYDEKTLPASAPKN
                     GHYKAYILGEGPDGVAKTPEWASQITGVPADKIIKLAREIGSTKPAFISQGWGPQRHA
                     NGEIATRAISMLAILTGNVGINGGNSGAREGSYSLPFVRMPTLENPIQTSISMFMWTD
                     AIERGPEMTALRDGVRGKDKLDVPIKMIWNYAGNCLINQHSEINRTHEILQDDKKCEL
                     IVVIDCHMTSSAKYADILLPDCSASEQMDFALDASCGNMSYVIFNDQVIKPRFECKTI
                     YEMTSELAKRLGVEQQFTEGRTQEEWMRHLYAQSREAIPELPTFEEFRKQGIFKKRDP
                     QGHHVAYKAFREDPQANPLTTPSGKIEIYSQALADIAATWELPEGDVIDPLPIYTPGF
                     ESYQDPLNKQYPLQLTGFHYKSRVHSTYGNVDVLKAACRQEMWINPLDAQKRGINNGD
                     KVRIFNDRGEVHIEAKVTPRMMPGVVALGEGAWYDPDAKRVDKGGCINVLTTQRPSPL
                     AKGNPSHTNLVQVEKV"
     gene            3199..3816
                     /gene="dmsB"
                     /locus_tag="ACHKTW_004103"
     CDS             3199..3816
                     /gene="dmsB"
                     /locus_tag="ACHKTW_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase iron-sulfur subunit
                     DmsB"
                     /protein_id="HFO6465025.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            3818..4681
                     /gene="dmsC"
                     /locus_tag="ACHKTW_004104"
     CDS             3818..4681
                     /gene="dmsC"
                     /locus_tag="ACHKTW_004104"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     DmsC"
                     /protein_id="HFO6465026.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLVVTMVLGVVFVWMMVRVYNSIDTVPTWYSIWTPMGFFLTMF
                     MGGPLLGYLLLSLAGVDGWAMRLLPAISVLALVVSGVMSVMQGTELATIHSSVQQAAA
                     LVPDYGALMSWRIVLLAVALCLWIAPQLKGYQPAVPLLSVSFILLLAGELIGRGVFYG
                     LHMTVGMAVAS"
     gene            complement(4717..5343)
                     /gene="ycaC"
                     /locus_tag="ACHKTW_004105"
     CDS             complement(4717..5343)
                     /gene="ycaC"
                     /locus_tag="ACHKTW_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate family cysteine hydrolase YcaC"
                     /protein_id="HFO6465027.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVPELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            5658..6806
                     /locus_tag="ACHKTW_004106"
     CDS             5658..6806
                     /locus_tag="ACHKTW_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO6465028.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLASFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITSMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFTAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
     gene            7016..8446
                     /locus_tag="ACHKTW_004107"
     CDS             7016..8446
                     /locus_tag="ACHKTW_004107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFO6465029.1"
                     /translation="MAGNVQEKQLRWYNIALMSFITVWGFGNVVNNYANQGLVVVFSW
                     VFIFALYFTPYALIVGQLGSTFKDGKGGVSTWIKHTMGPGLAYLAAWTYWVVHIPYLA
                     QKPQAILIALGWAMKGDGSLIKEYSVVALQGLTLVLFIFFMWVASRGMKSLKIVGSVA
                     GIAMFVMSLLYVAMAVTAPAITEVHIATTNITWETFIPHIDFTYITTISMLVFAVGGA
                     EKISPYVNQTRNPGKEFPKGMLCLAVMVAVCAILGSLAMGMMFDSRNIPDDLMTNGQY
                     YAFQKLGEYYNMGNTLMVIYAIANTLGQVAALVFSIDAPLKVLLGDADSKYIPASLCR
                     TNASGTPVNGYFLTLVLVAILIMLPTLGIGDMNNLYKWLLNLNSVVMPLRYLWVFVAF
                     IAVVRLAQKYKPEYVFIRNKPLAMTVGIWCFAFTAFACLTGIFPKMEAFTAEWTFQLA
                     LNVATPFVLVGLGLIFPLLARKANSK"
     gene            complement(8447..9355)
                     /locus_tag="ACHKTW_004108"
     CDS             complement(8447..9355)
                     /locus_tag="ACHKTW_004108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO6465030.1"
                     /translation="MRMNMSDFATFFAVARNQSFRAAGDELGLSSSAISHSIKTLEQR
                     LKIRLFNRTTRSVSLTEAGSNLYERLRPAFDEIQIMLDEMNDFRLTPTGTLKINAARV
                     AARIFLMPLLVGFTREYPDIKVELTTDDSLVDIVQQGFDAGVRLSGIVEKDMISVAIG
                     PPVKLCVAATPEYFARYGNPRHPHDLLNHQCVVFRYPSGKPFHWQFAKELEIAVAGNI
                     ILDDVDAELEAVLMGAGIGYLLYEQIKEYLDTGRLECVLEDWSTERSGFQIYYPNRQY
                     MSCGLRAFLDYVKTGQICQSQRHRPQ"
     gene            9455..10045
                     /locus_tag="ACHKTW_004109"
     CDS             9455..10045
                     /locus_tag="ACHKTW_004109"
                     /EC_number="1.6.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H oxidoreductase"
                     /protein_id="HFO6465031.1"
                     /translation="MQSERIYLVWAHPRHDSLTAHIADAIHQRAMERKIQVTELDLYR
                     RNFNPVMTSEDEPDWKNMDKRYSPEVHQLYSELLEHDTLVVVFPFWWYSFPAMLKGYI
                     DRVWNNGLAYGDGHKLPFNKVRWVALVGGDKESFVQMGWEKNISDYLKNMCSYLGIED
                     ADVTFLCNTVVFDGQELHASYYQSLLSQVRDMVDAL"
     gene            complement(10127..10867)
                     /gene="pflA"
                     /locus_tag="ACHKTW_004110"
     CDS             complement(10127..10867)
                     /gene="pflA"
                     /locus_tag="ACHKTW_004110"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HFO6465032.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
     gene            complement(11059..13341)
                     /gene="pflB"
                     /locus_tag="ACHKTW_004111"
     CDS             complement(11059..13341)
                     /gene="pflB"
                     /locus_tag="ACHKTW_004111"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFO6465033.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKQLEKIVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKLAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDVLNYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            complement(13396..14253)
                     /gene="focA"
                     /locus_tag="ACHKTW_004112"
     CDS             complement(13396..14253)
                     /gene="focA"
                     /locus_tag="ACHKTW_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HFO6465034.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWGQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
BASE COUNT         3492 a         3365 c         3698 g         3807 t
ORIGIN      
        1 gttgctgttc tgtgtctttc cactggcgca aggtgggggg aagcagctgg cttacgtggt
       61 gagcatatcg ttggtaaccg cgtaatgttc tttaacacaa agaatggaaa atcccgcgcg
      121 gttcctgttt ctgatacggt gctttcgttg ataaaaaccc gaaaaaccgg gctattgtac
      181 caggctgatt acatccgttt tcgggatatc cttcaggagg tgaaaccgga tttacccaaa
      241 ggtcaggcga ctcacgttat gcgtcacacg ttcgccacgc atttcatgat gaacggcgga
      301 aatatcgtta cgttacagcg aatccttggg cacgccacga ttcaacaaac gatgacgtat
      361 gcgcattttt caccggactt tttacaggat gcgattagct ttaatccgct ggctgatagt
      421 gtccataaac tgtccattga ctagttgtaa tttgcgttaa tttgctcccc tttttttgat
      481 ttagtcattg aaaaataagt aaatcatcaa ggggagtaag gtgtgttaaa tctcctgcgg
      541 gcgctttttt tgtctccctt tgataccgaa caataattac tcctcactta cacgtaatac
      601 tactttcgag tgaaaatcta cctatctctt tgattttcaa attattcgat gtatacaagc
      661 ctatatagcg agctgctata gaaataatta cacaatacgg tttgttactg gaatcaatcg
      721 tgagcaagct tgagtgagcc attatgaaaa cgaaaatccc tgatgcggta ttggctgctg
      781 aggtgagtcg ccgtggtttg gtaaaaacga cagcgatcgg cggcctggca atggccagca
      841 gcgcattaac attacctttt agtcggattg cgcacgctgt cgatagcgcc attccaacaa
      901 aatcagacga aaaggttatc tggagcgcct gtacagttaa ctgtggtagt cgctgcccgc
      961 tacgtatgca cgtcgtggac ggtgaaatca aatatgtcga aacggacaat accggcgatg
     1021 acaattacga cggcctgcac caggttcgcg cctgcctgcg tgggcgttcc atgcgtcgcc
     1081 gtgtctacaa tccggaccgc ctgaaatatc cgatgaaacg agtcggggcg cgcggtgaag
     1141 gcaaattcga gcgcattagc tgggaagaag cctacgacat catcgcgacc aatatgcagc
     1201 gcctgatcaa agagtacggc aacgagtcta tctatctgaa ctatggcacc ggtacgctgg
     1261 gcggcaccat gacccgctcc tggccgccgg gaaataccct ggtcgcgcgg ctgatgaact
     1321 gctgcggcgg ctatctgaac cattacggcg actactcctc cgcgcaaatt gcggaaggtt
     1381 tgaactatac ctacggcggc tgggcagatg gtaacagccc gtcggatatc gaaaacagta
     1441 agctggtagt gctgtttggt aataaccctg gcgaaacgcg aatgagtggc ggtggggtga
     1501 cttactatct tgaacaggca cgccagaaat ctaatgcccg catgatcatc attgatccgc
     1561 gctataccga caccggtgcc gggcgcgaag atgagtggat ccctattcgt ccgggaacag
     1621 atgccgcact ggttaacggt ctggcgtacg tcatgatcac tgaaaacctg gtggatcagg
     1681 cattcctcga taaatattgc gttggctacg atgagaaaac cctgccagcc agtgcgccga
     1741 aaaatggcca ctataaagct tatattctgg gtgaagggcc agatggcgtg gctaaaacac
     1801 cggaatgggc ctcacaaatc accggtgttc cggcagacaa aatcatcaaa ctggctcgtg
     1861 aaatcggcag taccaaaccg gcgtttatca gccagggatg gggcccgcag cgtcacgcta
     1921 acggtgaaat cgcaacccgt gctatctcga tgctggcgat tctgaccggt aacgttggta
     1981 ttaacggagg caacagcggc gcgcgtgaag gttcatacag cttaccgttt gtccgtatgc
     2041 cgaccttgga aaacccgatc cagaccagca tttcgatgtt tatgtggacc gatgccattg
     2101 aacgtggccc ggaaatgacg gcgctgcgtg atggtgtacg cgggaaagat aagctggatg
     2161 tgccgatcaa aatgatctgg aactatgccg gtaactgcct gattaaccag cattctgaaa
     2221 tcaaccgtac ccatgaaatc cttcaggatg ataagaagtg cgagctgatt gtggttatcg
     2281 actgccacat gacctcatcg gcgaaatatg ctgacatcct gctgcctgac tgcagcgctt
     2341 ccgaacagat ggactttgca ctggatgcat cctgcgggaa tatgtcttac gtgattttca
     2401 acgatcaggt gattaaaccg cgctttgaat gtaagaccat ctatgaaatg accagcgaac
     2461 tggcaaaacg tcttggcgtt gagcaacagt ttactgaagg ccgtacccag gaagagtgga
     2521 tgcggcatct gtatgcccag tcgcgggaag cgattcctga actgccaacg tttgaagagt
     2581 tccgcaagca ggggatcttt aaaaagcgcg acccacaagg gcatcacgtt gcttataaag
     2641 ccttccgtga agatccgcag gcaaacccac tgactacgcc atcgggcaaa attgagattt
     2701 attcgcaggc gctggctgac attgccgcta cctgggaatt gccagaaggc gatgtgatcg
     2761 atccactgcc gatctacacg ccgggctttg aaagttatca ggatccgctg aacaaacagt
     2821 atccgctgca gcttacgggt ttccactata aatctcgcgt tcactcaact tacggcaacg
     2881 ttgatgtgct gaaagcggct tgccgtcagg aaatgtggat caacccgctt gatgcgcaaa
     2941 aacgcggtat caacaacggc gataaagtca ggatctttaa cgatcgtggt gaagttcata
     3001 ttgaggcgaa agtgacgcca cgaatgatgc cgggtgtggt cgcactgggt gaaggtgcct
     3061 ggtatgaccc ggatgcaaaa cgtgtcgata agggcggttg tattaacgta ctgaccactc
     3121 aacgtccgtc tcctctcgct aaggggaatc cgtcacatac aaaccttgtt caggttgaaa
     3181 aggtgtaagg agtaaccgat gacaacccag tatggatttt ttattgattc cagccgttgc
     3241 accggttgca aaacctgcga gctggcctgt aaagactaca aagatttgac gccagaagtc
     3301 agcttccgcc gcatttatga atatgctggc ggcgactggc aggaagataa cggtgtctgg
     3361 caccagaacg tgtttgccta ctatctgtcg atttcatgta accactgcga agatccggct
     3421 tgtactaaag tctgcccgag cggtgcgatg cataaacgcg aagatggttt tgttgtggtc
     3481 gatgaagatg tatgcattgg ctgccgctac tgtcatatgg cttgcccgta tggcgcaccg
     3541 caatataacg aaacgaaagg ccatatgacc aaatgcgatg gttgttatga ccgtgttgcg
     3601 gagggtaaaa agccgatctg tgttgaatct tgcccgctgc gggcgctgga tttcggtcct
     3661 atcgacgagc tgcgtaaaaa acatggcgat ctggcggccg ttgcgccgtt gccgcgagct
     3721 cactttacca aaccgaatat tgtgatcaaa cccaatgcca acagccgccc gaccggggat
     3781 accactggct acctggcaaa cccgaaggag gtgtgagatg ggaagtggat ggcatgaatg
     3841 gccgctgatg atcttcacgg tcttcgggca atgtgtagcg ggtggtttta tcgttctggc
     3901 tttggcgctg ctcaaaggcg acctgcgagc agaagcccag cagcgtgtta tcgcctgcat
     3961 gtttggctta tgggtgctga tgggcattgg ctttatcgcc tctatgctcc atcttggttc
     4021 accgatgcgc gcctttaact cgctcaaccg ggtaggggct tcagcactca gtaacgaaat
     4081 cgccagcggt tcgatctttt ttgctgtagg cggcatcggc tggttgctgg caatgctgaa
     4141 aaagctgtca ccggcattgc gtacgctatg gctggtggtg acgatggttc ttggcgtcgt
     4201 ctttgtctgg atgatggtgc gtgtgtataa cagcattgat accgttccga cctggtacag
     4261 catctggacg ccgatgggct tcttcctgac gatgttcatg ggcggcccgt tactgggtta
     4321 cctgttgctg agtctggctg gcgtcgatgg ctgggcgatg cgtctgctgc cagccatttc
     4381 tgtactggca ctggtggtga gtggtgtgat gtcagtgatg cagggcacag agctggcaac
     4441 tattcatagt tctgtgcagc aggccgcagc gctggtgccg gactatggtg cgctgatgtc
     4501 ctggcggatc gtgcttttgg ccgttgccct gtgcttgtgg attgcaccac agctaaaagg
     4561 ttatcagcct gcggttccgc tactatccgt ctcattcatt ctgcttctgg caggggaatt
     4621 aatcggtcgc ggcgtattct atggtttgca tatgaccgtg gggatggccg tcgcaagcta
     4681 atcagtaaca accggggttt cggccccggt tctcttttat ttctgcttcg ttaacgtgtc
     4741 ataactggtc atcaaattac gataatcagg aatatggtta gagaacaacg tcgccagtcc
     4801 ttcaatatca ttacgccagt cacggtgcag ctcgcaagcc acgccgaacc atgtcatcaa
     4861 ttgcgcacct gcttgcgaca tgcgatccca tgccgaatgc cgggtaattt cattaaaggt
     4921 ccctgacgcg tcagtcacaa caaagacatc aaacccttct tcaatggctg aaagcgccgg
     4981 gaatgccacg caaacttcgg ttaccacacc ggcaataatt aactgttttt tacctgtcgc
     5041 tttgaccgct tttacaaaat cttcgttatc ccaggcgtta atatttcccg ggcgagcaat
     5101 gtaaggtgca tcggggaatt gtgctttcag ttctggaact aatgggccgt taggaccggt
     5161 ttcaaaactg gtggtaagaa tagttggtaa gttgaaatac ttagccaggt cacccagcgc
     5221 cagcacgtta tttttaaact tatcgggttc gatatcccgt acaagggaaa gtaaaccagc
     5281 ctggtgatca acaagcaaaa cggcagcatc atttttatca agacgaacat acggtttggt
     5341 catcctcttc tcctttcgag agtcagtggt acgtctgaaa tcggtcagac gcgatgcatt
     5401 gctctgaaag catagacggg aaatatgagt ttgctgtgac catgaaattt ttcgactgaa
     5461 cgaagcgtat gaaatttgtg ttagatcaat aaaaacaatc agatgagatt aatcgtctct
     5521 tcagcattag cgttatgaat gagtaatata aatcgataat accgccgctg cgatatgcgc
     5581 gtattttcac ccattgacaa tgtttttggc ggtggcatga tgcgcatgaa atttgaactt
     5641 cctcacggtt ttaattcatg tccacgtata cccggcctgt catgcttttg ctgtctggcc
     5701 tgcttttgtt gactctggcg atcgcggtgt taaatacact cgtgccgctt tggctcgccc
     5761 aggaacacat gtccacatgg caggtaggcg ttgtcagctc atcctatttt accggcaacc
     5821 ttgtcggtac attgctgaca gggtatgtca ttaagcgcat tggctttaac cgcagctatt
     5881 atctggcctc cttcattttt gccgctggct gtgccggcct tggcctgatg attggattct
     5941 ggagctggtt ggcttggcgt tttgtcgcgg gcgtcggctg tgccatgatt tgggtggttg
     6001 ttgagagcgc gctgatgtgc agtgggacgt cacgtaaccg tgggcgtttg cttgctgcgt
     6061 atatgatggt ttattacgtg ggaacgtttt taggccagtt actggtcagc aaagtttcaa
     6121 ccgagctgat gtccgtattg ccgtgggtta caggtttgac gttggcaggg atcttaccgc
     6181 tgttgtttac gcgtgtgctg aatcagcagg ctgaaaacca tgattcgacg tcaattacgt
     6241 caatgctaaa actccgtcag gcgcggcttg gcgtgaatgg ctgcattatc tcaggaatcg
     6301 ttctgggatc tctatatggc ctgatgccgc tgtacctcaa tcacaaaggg gtgagcaatg
     6361 ccagcattgg tttctggatg gcggtactgg tcagtgcggg tatccttgga caatggccga
     6421 ttggacgtct ggcggataag tttggtcgac tgctggtgtt gcgtgttcag gtctttgtcg
     6481 tcattctcgg cagtatcgcg atgcttagcc aggcggcgat ggccccagcg ttattcatcc
     6541 tcggtgccgc tggctttacg ctatatccgg tggcgatggc atgggcttgc gagaaagttg
     6601 aacatcatca actggtggcg atgaaccagg ccttactgtt gagttatact gtgggaagtc
     6661 tgcttggccc gtcatttacc gctatgctaa tgcagaattt ctccgataat ttattgttta
     6721 tcatgatcgc cagcgtatcg tttatctatt tgctgatgct gctgcgcaac gccggtcata
     6781 cgccgaaacc cgttgctcac gtgtaaatga attcaagcag agtgtgaact tactgtttca
     6841 cactctgctt ttttgtttct tctatctgac ttgctttatt ccaaatttta ttcgtttaaa
     6901 aataaaatgt gcagcaggtt ataattttgc atttcgctat ttccgcactt cttatttgcc
     6961 gcgcataatc cctcgtttta cagatgcccc tttaattttg gcgaaggatt tgtctatggc
     7021 tgggaatgtt caggaaaaac agttgcgatg gtacaacatt gcgctgatgt cttttatcac
     7081 tgtctggggt tttggcaacg ttgttaataa ctatgccaac caggggctgg tggttgtttt
     7141 ttcatgggtg tttatctttg cactctattt cacaccttat gcgctaattg ttggtcagtt
     7201 aggctcgacc ttcaaagatg ggaagggcgg ggtcagtacc tggattaaac acacgatggg
     7261 acccggactg gcttatctcg ccgcgtggac ctactgggtg gtgcatattc cctatctggc
     7321 acaaaaaccc caggcaattc tgattgcgct cggttgggcg atgaaaggcg acggttcgct
     7381 aatcaaagaa tattcagtcg tagcgttaca ggggttaacg ctggtgctgt ttatcttctt
     7441 tatgtgggtt gcttcacgcg gtatgaaatc gctgaaaatc gtcggttctg tggcagggat
     7501 tgcgatgttt gttatgtcgc tcctgtatgt ggcgatggcg gtaaccgcgc ctgcgattac
     7561 tgaagtgcat attgcgacca caaacattac ctgggaaacg ttcattcctc atatcgactt
     7621 tacctacatt accactattt caatgctggt tttcgcggtt ggcggagcag agaagatttc
     7681 tccttacgtt aatcaaacgc gcaacccagg aaaagaattt ccaaaaggga tgctatgcct
     7741 ggcggtgatg gttgcggttt gtgccattct gggctcactg gcgatgggga tgatgtttga
     7801 ctcgcgtaat atcccggatg acttaatgac taacggtcaa tattacgcct ttcagaagct
     7861 gggcgagtat tacaacatgg gtaatacttt aatggtgatt tacgccattg cgaataccct
     7921 gggacaagta gctgcgctgg tattctcgat tgatgccccg cttaaagtgc tattaggtga
     7981 tgctgacagc aaatatattc cagccagttt atgtcgtacc aacgcttctg gtacgcccgt
     8041 taatggctat tttctgaccc tggtgctggt ggcaattctg attatgctgc cgactctcgg
     8101 catcggtgat atgaacaatc tctataaatg gctgttgaat cttaactcgg tagtgatgcc
     8161 gctgcgttat ttatgggtat ttgttgcatt tattgcagtc gttcgcctgg cgcagaaata
     8221 taaaccagaa tatgtcttta ttcgtaataa gcctctggca atgaccgtcg ggatttggtg
     8281 ttttgccttt accgcctttg cctgcttgac ggggatcttc ccgaaaatgg aagccttcac
     8341 tgcagagtgg accttccagt tggcgctgaa tgttgcaacg ccgtttgtgc tggtaggatt
     8401 aggactgata ttcccgctgc tggcgcgtaa agcgaatagt aaataattat tgtggtcggt
     8461 gacgctgact ctggcagatc tgacccgttt ttacataatc gagaaatgcg cgcaagccgc
     8521 aggacatata ctggcggttt ggataataga tctggaagcc tgaacgctct gtgctccagt
     8581 cttccagtac acattctaaa cgaccagtat caaggtactc cttgatctgt tcatagagca
     8641 gatacccgat accagcaccc atcaaaacgg cctccagttc agcatcaaca tcatccagaa
     8701 tgatatttcc tgcgacagca atttccagct cctttgcaaa ttgccagtgg aaaggtttgc
     8761 cgctgggata acgaaaaaca acacattgat gattaagcag atcatgagga tggcgggggt
     8821 tgccatatct tgcaaagtac tctggcgttg ctgctacaca taatttcact ggtgggccga
     8881 tcgccacgct gatcatatct ttttcgacga tgccactaag tcgaacgcca gcgtcaaagc
     8941 cctgttggac gatatcgaca agagaatcgt cagttgtgag ctcgacttta atatcaggat
     9001 attcacgtgt gaaaccaacc agtaaaggca ttaaaaatat acgtgcagct acccgggcgg
     9061 cattgatttt gagcgttcct gtcggtgtca gacgaaaatc attcatctca tctaacatga
     9121 tctgaatttc gtcgaaagct gggcgcaatc gttcatagag attagaaccc gcttccgtta
     9181 acgatacgct tcgggttgtc cgattgaata agcgaatttt aagacgttgt tccagtgttt
     9241 taatactatg gctaatggcg gacgaggata agcctaattc atcgcccgct gcacgaaagc
     9301 tttgattacg ggccacggca aagaaagtgg caaagtcaga catattcatc cgcattagtg
     9361 aaaatccttc attaactcat cctgaatcat cacgtttaca ggattatgcc gcctgcgtaa
     9421 agtgcgctcc agaacttaac gtggaggtaa aattatgcag tctgaacgta tttatttggt
     9481 atgggcccat cctcgtcatg attcattgac cgcacatatt gctgatgcga tccatcagcg
     9541 ggcaatggag cggaaaatac aggtgacgga actcgattta tatcggcgta atttcaaccc
     9601 ggtgatgacg tcggaagatg aaccagactg gaagaatatg gataaacgtt attctccaga
     9661 ggttcatcag ctttattcag agctgcttga acatgacacg ttagtggtgg tttttccttt
     9721 ctggtggtac agcttcccgg caatgctaaa aggatatatt gacagagtat ggaataatgg
     9781 gctggcttat ggagatgggc acaaattacc attcaataaa gttcgttggg tggcgctggt
     9841 tggaggagac aaagaatcat ttgtccagat gggctgggaa aaaaatataa gcgattattt
     9901 gaaaaatatg tgcagttatc ttggtattga agatgccgat gtcactttct tgtgtaatac
     9961 agtggtattc gatgggcaag aacttcatgc gagctattat cagtcgttat tatctcaggt
    10021 acgggatatg gtagatgcac tataaaatgt gttaaaaacg ctgtagcaga atgaagcgcg
    10081 gaataaaaaa gcggcaactc aataaagttg ccgctttacg gggaaattag aacattacct
    10141 tatgaccgta ctgctcaaga atgcctttca cgcgttccat ggtctctttc ttaggtggtt
    10201 taacaccgtc gagtttgtac tcttcaccca ttgccaccca tttgtgtttg cccagctcgt
    10261 ggtaggggag gagttcgatt ttctcaacgt tgcccatatc acgggtaaat tcaccgaggc
    10321 gatgcgctga atcgtcatcg tcagaccagc ctgggacaac aacgtagcgg atccacacct
    10381 tcacattttt gttcgccaga tatttggcga actccagcgt gcggtggttg gaaactccaa
    10441 ccagattttg gtggatctcg tcgttcatct gtttgagatc gagcattacc aggtcggtta
    10501 cttccagcag ttcatcaatc accggatcgt aacgacgaac aaaaccgttg gtgtccagac
    10561 aggtatgaat gccttctttt ttgcaggcgc ggaaccagtc acgaacaaac tcagcttgta
    10621 ggattgcctc accgccggat gcggtaacgc cgccgccgga agcgttcata aagtggcgat
    10681 aggtcaccac ctctttcatt aaatcttcaa cggtaacttc tttaccgcca tgcgtatccc
    10741 aggtgtcgcg gttatgacaa tacaggcagc gcatcaggca gccctggaaa aaggtgataa
    10801 agcgaatacc tgggccgtct acggttccac aggattcaaa ggagtgaatg cgaccaataa
    10861 ctgacattgc ggtgtttctc cagatgtggc ccatctgagg ccgtgttggt gcgcagctcg
    10921 aaggctacgt cgagtctgtt ttggcagtca ccttaaagta tagatagctg acaaaaaagg
    10981 ctctcgtgct aaaaaaggcc ccactttcgt ggagccttta ttgtacgctt tttactgtac
    11041 gatttcagtc aaatctaatt acatagattg agtgaaggta cgagtaataa cgtcctgctg
    11101 ctgttcttta gtcagcgagt tgaaacgtac tgcgtagcca gatacacgga tggtcagctg
    11161 cggatatttt tccgggtttt ccatcgcgtc gagcagcatt tcacggttca tcacgttaac
    11221 gttcaggtgc tgaccacctt cgatggatgc ttcgtggtgg aagtaaccat ccatcagacc
    11281 agccaggttg gtcttacgaa cttcgtcgtc tttacccagt gcgttcggaa cgatagagaa
    11341 ggtgtaggag ataccatctt tagcgtaagc aaacggcagt ttagcaacgg aagtcagaga
    11401 cgctacagca cctttctggt cacgaccgtg catcgggtta gcacccggtc cgaacggcgc
    11461 gccagcacga cgaccgtctg gggtgttacc agttttctta ccatacacaa cgttagaagt
    11521 gatggtcaga acagactgag tcgggatagc gtcacggtag gtgtgcagtt tctgaatttt
    11581 cttcatgaaa cgttctacca ggtcaacagc caggtcatct acacgcggat cattgttacc
    11641 aaactgcggg tattcgcctt cgatttcgaa gtcgatagcc agaccgtctt cgtcacgaat
    11701 cggtttaact ttcgcatatt tgattgcaga cagggagtca gcagcaacgg acagaccagc
    11761 gataccacac gccatggtgc ggataacgtc acggtcgtgc agcgccatca gagaggcttc
    11821 gtagctgtac ttgtcgtgca tgtagtggat gatgttcagt gcagtgatgt actgtttagc
    11881 cagccagtcc atgaagtgat ccatgcgctc catcacttca tcatagttca ggacatcgcc
    11941 tttgatcggt tcagacttcg gaccaacctg cattttcagt ttttcgtcaa cgccgccgtt
    12001 gattgcgtac agcatggttt tcgccaggtt tgcacgcgca ccgaagaact gcatttgttt
    12061 accaacgatc atcgggctta cgcagcaagc aatagcgtag tcatcgttgt tgaagtccgg
    12121 acgcatcagg tcatcgttct catactgcag agaagaggtg tcgatggaca ctttagcggc
    12181 gaatttcttg aagttcagcg gcagtttttc agaccacaga atggtcatgt tcggttccgg
    12241 agacggaccc atggtgtaca gggtgttcag gaaacggaag ctgtttttgg taaccagggt
    12301 acgaccgtcg aggcccatac caccgataga ttcggttgcc cagatcgggt cgccagagaa
    12361 cagttcatcg tattccggag tacgcaggaa gcgaaccata cgcagtttca tgaccaggtg
    12421 gtcaaccatt tcctgcgctt cttgttcggt gatcttgcca gctttcaggt cacgttcgat
    12481 gtacacatcc aggaaggtgg aggtacgacc gaaggacatt gcagcaccgt tctgagactt
    12541 aacagcagcc aggtagccga agtaagtcca ctggatagct tcctgagcgt tggtagccgg
    12601 accagagatg tcgtagccgt atttcgcagc catttctttc atctgaccca gagcgcggtg
    12661 ctgttcagcg atttcttcgc gcagacggat agtctgttcc aggtttacgc cgttttccag
    12721 atcagcctgc agagaagtga actgtgccag tttgtctttc atcaggtagt cgataccgta
    12781 cagcgcaacg cgacggtagt caccgatgat acggccacgg ccatatgcat ctggcagacc
    12841 ggtcagaaca ccagatttac ggcaacgcag gatgtccgga gtgtaaacgt cgaacacgcc
    12901 ctggttgtga gttttacggt attcagtgaa gatttttttg atcatcggat ccagttcgcg
    12961 gttgtacgct ttgcaggaac cttcgatcat tttgatacca ccgaacggga taagagcacg
    13021 tttcagcgga gcttcagtct gcagaccaac gattttctca agctgcttgt tgatgtagcc
    13081 agcgtcgtga gaggtgatgg tggaagcaac agcggtgtca aagtcaactg gcgcgtgagt
    13141 gcggttttcc agtttaacgc cttccattac tttgtcccac agggtggtgg tcgcttcagt
    13201 agcgccagcc aggaaggact cgtcaccctc gtacggagtg tagtttttct gaatgaagtc
    13261 acggacgttt acttcattct gccagtcacc tttggtaaaa ccttcccagg ctgtggctaa
    13321 cttttcatta agctcggaca tgtaacacct accttcttaa gtggattttt tatttactgc
    13381 gtacttcgac aaccattaat ggtgatcgtt ttcacgcagg taaatgaccc agtatgtcaa
    13441 cccaaccaac aaaccaccgc cgataatatt accgatcgta accggaatca ggttatcagt
    13501 gatgaagttc atcacggtca ggtgagaaaa attttccggt gcagaaccga cagcggtcca
    13561 gaattccgga gatgcgaagt cgcggattac aatacccatc gggatcataa acatgtttgc
    13621 gatactgtgc tcaaaaccgc tggcaacaaa catcgcgacc ggcagcacca taatgaacgc
    13681 tttgtccatc aggctacggc cagaataact catccatact gccagacata ccatcaggtt
    13741 tgccaggata ccaaggcaga cggcctcaat aaaagtatgg tgcactttgt ggtcggcggt
    13801 ttgtaggacg tttagtcccc attgaccatt tgcggtcata tactcgccgg aaagccacat
    13861 taaaagtaca aacagcagtg cgccgaccag gttgccaaaa tagacattta gccagttttt
    13921 cgccaactga ccccaggtga tgcgcccact cgccttagca acaacaatca acactgtgga
    13981 agtaaagaga tcggctccgc agacaacaca aagaatcagc cccagagaga agcaaatgcc
    14041 gccaaccagt tttgccatgc cgaagggcat tgtgcctgtg ccagtggttg ctgtgatata
    14101 gaagacgaat gcgattgaga tgaaaacacc ggcggtaatc gccagataga aagtcttaag
    14161 cggatgtttc gttgctttat agacacccgc ctcttcggcc actttggcca ttgcagcagg
    14221 aagtaaaaga tcaaaagggt tgtcagcttt cacactaact ctctctttat taagtcggcg
    14281 acgagatact aacaaagcat tatagatgag aaattgatat agatcatatc tcgcctggct
    14341 tataggcccg taactcgcat gg
//
LOCUS       DAZFQD010000137        14351 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.137, whole genome shotgun sequence.
ACCESSION   DAZFQD010000137 DAZFQD010000000
VERSION     DAZFQD010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14351
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.137"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..822
                     /locus_tag="ACHKTW_004113"
     CDS             <1..822
                     /locus_tag="ACHKTW_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001682040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase C-terminal domain-containing
                     protein"
                     /protein_id="HFO6465035.1"
                     /translation="LDEMTCGLQPGDLILLAARPSMGKTALGLTLCLNALRERTDETV
                     FLFSIEMSREQLILRTLSMLSRVELTRLRSSMMDDEEWARLTATMGEFMQEGTFAGEG
                     NRLIIDDTSSQTPSSLRASARRYVRLYGRPSLIMVDYLQLIRSPDQENRTQEIAEISR
                     ALKSLGKELGCPVLALSQLNRQVEQRADKRPNNGDLRDSGALEQDADLIMFIYRDEVY
                     NPGTLDKGVAEIIVSKQRQGPTGTIRVSFDGRYTLFSPFERGWNFDRSGGNAYVN"
     gene            812..2437
                     /locus_tag="ACHKTW_004114"
     CDS             812..2437
                     /locus_tag="ACHKTW_004114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001682041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB family protein"
                     /protein_id="HFO6465036.1"
                     /translation="MSIKRNHSLDLGAAIMQPGRQASKTGNVVALGETPMVLTLDQLR
                     PNPDNPRTTRNPRYDDIKSSIFSRGLDTVPKVTRIPESEPDIYIFSDGGNTRYQILRE
                     LWEETGEQRFYRIHVLFKPWPGRLQCVIGHLAENEVRGELTFIEKAQGIHKARMIYEE
                     SLQRTVTIRELASLLTDEGLPVNHSSVSRMEHALKYLYPWIPDLLESGMGSPQVRALL
                     SLRQDAERIWGQFAVAAENDVDFDRVFGECCGKFNSPELWSMEMFRDELIGDLLKALP
                     HPVLDYDRWLLELDPRERNRRHHFGEPEPVLMPADSPPEPGAQVASGGATEVAQVVAA
                     TPADRESVMDTAQSGAEKEDRPVADKCERRRKEVQPDMYGGEDSYSGDCGEDATGAST
                     DIPIMPEDNAMQEGGEFRQASAFTGAEQDDIESLQNEAFHLAWELASHTGCVDEIAMD
                     RESELSAGFGAAEANCSPVMAFLVRLTGEEVLRVNPVSLDALLIGGSTEEDLPLLDDE
                     HAGKLLRLLGVLRRLRGLQRMVEDESEEGRDEC"
     gene            2427..3158
                     /locus_tag="ACHKTW_004115"
     CDS             2427..3158
                     /locus_tag="ACHKTW_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000990583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2786 domain-containing protein"
                     /protein_id="HFO6465037.1"
                     /translation="MNADAARQDQLIKRIRKLLALSRNNSNPHEAGQALSRAQALMRK
                     HGITELDTDISVIKTSSSKGAPSEAKKVPVWMGRLMHVVAEAFGCHTYLDWRITTGRY
                     WRRVVIFYGFGERPVVAAYAFDVLCRQMQKATKAYLSTQSRTLKLSTRRARAAMFRDG
                     WVYGVRDVVDAFPLTEDETRVMKTWLKRQQFEGMAAFRNVKDCNGGDRARWQGYSEGK
                     KATLHHGVGSRNLRPESLISGGGEV"
     gene            3155..3733
                     /locus_tag="ACHKTW_004116"
     CDS             3155..3733
                     /locus_tag="ACHKTW_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2857 domain-containing protein"
                     /protein_id="HFO6465038.1"
                     /translation="MSNNLSQATNGLLMQLVMDLKSGYLRRCEALGLSREEMQMLQGL
                     SIEEIHYLSNSEVSVLRLDINHNNLVRMLQQARTEQKRLQRIDRALALGGSIELMAFY
                     FGLSSVDVAARRRISGIDVRPGRGITLSDDENSELWRLWQKAGINDVESVDGLDVMML
                     CAEQMNIPLTAIWHAVRGWHTTGNPDQVRSAS"
     gene            3730..3978
                     /locus_tag="ACHKTW_004117"
     CDS             3730..3978
                     /locus_tag="ACHKTW_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6465039.1"
                     /translation="MKMTELVPGVLTSPGVPPASQRKAVELVNRFRRGERNYHELKER
                     GFGYFKIDVGPDWRLLSRNKGLTWALLSHERYNNMRLK"
     gene            3988..4245
                     /locus_tag="ACHKTW_004118"
     CDS             3988..4245
                     /locus_tag="ACHKTW_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6465040.1"
                     /translation="MSNAARLQLGFSPFSKTIVLAKMRNLDDGTSLRVGNDPGRDVTN
                     EAAQLVWHLVMAEGGEIMWEMDDGSRMVLKAESQEATSEQD"
     gene            4232..4966
                     /locus_tag="ACHKTW_004119"
     CDS             4232..4966
                     /locus_tag="ACHKTW_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001682044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFO6465041.1"
                     /translation="MNKIDYQALRKAAQNYQSTLAWYQSIPDSPNAERDCDAALAAFK
                     HHIRHREVDIISGLLDELDEKQQYIKSRDQENEDIALTVGKLRVELEEVKQHAEELSE
                     TNAVRNQWRPDICPITGRAFFMWIEHPTLGNVPTYGGPLDSYTIPTKDGDGEFSCERY
                     DHDFGGWVESECPGLYLIDDKEQCRVYELEERVKELDAREISLPERSSMLHRTDFNEA
                     YHTVMAYKVSEVIAAIRVAGIRIKGE"
     gene            4968..5146
                     /locus_tag="ACHKTW_004120"
                     /pseudo
     CDS             4968..5146
                     /locus_tag="ACHKTW_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305741.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5480..5608
                     /locus_tag="ACHKTW_004121"
                     /pseudo
     CDS             5480..5608
                     /locus_tag="ACHKTW_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309545.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5899..6114
                     /locus_tag="ACHKTW_004122"
     CDS             5899..6114
                     /locus_tag="ACHKTW_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001682047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6465042.1"
                     /translation="MRKISGMLNLEKARMQLRVLLSGPVPAITPMAHAVAIKVVLDAL
                     DASERRVAELEQVCTAAGIRIKGEQVK"
     gene            6111..6338
                     /locus_tag="ACHKTW_004123"
     CDS             6111..6338
                     /locus_tag="ACHKTW_004123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001682048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6465043.1"
                     /translation="MKLKMQTISGPVIIDSNEVTQFYPDHESGGELTAVEYFTDEGRC
                     TTRVRHSFYQVAEALASAWHVDDASKRNRRG"
     gene            6602..7888
                     /locus_tag="ACHKTW_004124"
     CDS             6602..7888
                     /locus_tag="ACHKTW_004124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001682049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STY4528 family pathogenicity island replication
                     protein"
                     /protein_id="HFO6465044.1"
                     /translation="MALPAESLIAHTLRKMKASMAEKASREEGRIRSGLLFTGNVHDA
                     VPRRLLLDTRLSPLDKTAWMMIRLHALSNEGAVFPTYDELQLQLASPGSGKASRETVS
                     RVLTMLRLTGWLSLCKRVRDEKGRIRGNIYALHDEPLGCCDAEKFDPRYLDMVLDSAC
                     KNRNKTIRYTAREVLSDIKNEPGMRHYHSHITLFESRMGAAQTPSQMAARQRVNPAVN
                     GRAAKSGSESAQPPVCASVTELSAQVTELCTAVTEPGEKTEVTQRSTDSEQPGKSVGY
                     HSVRKPNHYVRNITHSMKKNTYVLPDALKSQLSVEDTGMLTKQLQALPETLAEGVLDS
                     LSQMLSKNTLQNPVGWMLAVMKRAREGNFNSPDMSRSGTIQSQPARPSSQSGHGCDER
                     ERRRREVASPEKARNIVAEFRASIQRANAARLAMRG"
     gene            complement(7897..8382)
                     /locus_tag="ACHKTW_004125"
     CDS             complement(7897..8382)
                     /locus_tag="ACHKTW_004125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6465045.1"
                     /translation="MQVVSYTVVAVPPSLVFCTVVAVPPSLVFCTVVAVPPLLIFYTV
                     VAVPPSLIFCTVVAVPPSLIFCTVVAVPPLLIFYTAVPVAPSIIFCTVVAVPPSLVFC
                     TVVPVPPSLVFCTVVAVPPSLIFCTVVAVPSSLIFYTVVAVAPLLIFCTTVTVPPSLN
                     L"
     gene            8526..9206
                     /locus_tag="ACHKTW_004126"
     CDS             8526..9206
                     /locus_tag="ACHKTW_004126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001682051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL_4669 family integrating conjugative element
                     protein"
                     /protein_id="HFO6465046.1"
                     /translation="MTDEKKAEPGVLKSTLSINLHTYYAIRLWEGRKPEPGADKSERR
                     RRIIGMPGVISRAKIASKDSENDNPWADMFLFNLEEKLSKSKQEIDLLVEQLNEVLRN
                     VPGDIIISGVASTSPVNIGVHSRSPLGYKSVWLLVGYDELVLKAFQAFHYGLIARARR
                     DELLNAGGHAVRQICALAQSYKTVPATRSDISSGSQKGKEAISRYGMPDPDVLSGKKR
                     SSFSAPLK"
     gene            9219..11228
                     /locus_tag="ACHKTW_004127"
     CDS             9219..11228
                     /locus_tag="ACHKTW_004127"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001682052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_function: GO:0098847 - sequence-specific single
                     stranded DNA binding [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0051304 - chromosome separation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFO6465047.1"
                     /translation="MRLYLCEKPSQASDIARVLGAARRGEGLIEGNDFVVTWAFGHLL
                     EQAPPEAYGEQYGSPWSIEVLPVIPEEWQMVVKDKIARQFNVIRRLLGKADEVVIATD
                     ADREGEIIARELLDYCGYTGVVRRLWLSALDESSIREALGRILPGEKTARLYEAGKGR
                     SRADWLTGINLTRLYTMKARECGVSGVLSIGRVQTPTLAMVVRRDAEIKNFIPVPYWQ
                     LNLWLEKDNVRFRAVWVAAEQYCDAEKRCVNVQAATSVQSQCRQAGVAVVREVSSKRE
                     KTPPPLCFSLGTLQEVCSKKFGLGAQAVLDIAQSLYEKHKATTYPRTDCGYLPVSMHK
                     EVPEVLAAVANTDPDIAPELAKLSPDIVSRVWNDKKITAHHAIIPTRQAFNIGALSDN
                     ELRVYRLIRQHYLAQFLPVQEADVTEATFVVAGQLFGTRGRVEVVKGWRVFIADAPHD
                     EDEGGDTEQTMPLPVLAKGDECRLVEAELKEMKTTPPRHYTEGTLIAAMKNAAGMVSD
                     PALKKVLRENAGLGTEATRAEILETLFTRKYIEKQGKHIRATQLGAELIAGLPEVLTS
                     PGTTALWEQALDDIAQGKLSLASFMEKQKQWLNHLVSGARRQQLQITAPVTPVCPLCQ
                     GRMRKLKGKNGEFWSCQRYPECKGVISEQKTSHSKRGGRKIKKRG"
     gene            11874..12338
                     /locus_tag="ACHKTW_004128"
     CDS             11874..12338
                     /locus_tag="ACHKTW_004128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016151303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STY4534 family ICE replication protein"
                     /protein_id="HFO6465048.1"
                     /translation="MSATNTSSAKAEYFNLTVKGLGYLSNVRQVNHQNGSFLCCVINA
                     LSGPTDNPSYTRFDVTVVGKDATTLISRCQKAVDEDRKVLLGFALSNLATDIFTLNKG
                     EHAGEQRVSLKARLIKVDWIKIGQEQVYKAEKAESLPPQNGTTKRQYAENSF"
     gene            12432..12635
                     /locus_tag="ACHKTW_004129"
     CDS             12432..12635
                     /locus_tag="ACHKTW_004129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001682054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6465049.1"
                     /translation="MNRQNGILAAAAGYTLPLEILKSTRGYYIGTQCSEGPVSRESEE
                     YFKKHDQAEQALKNGTWTQRCDW"
     gene            12648..13205
                     /gene="ssb"
                     /locus_tag="ACHKTW_004130"
     CDS             12648..13205
                     /gene="ssb"
                     /locus_tag="ACHKTW_004130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004115562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFO6465050.1"
                     /translation="MASRGVNKVILLGHLGQDPEVRYFPNGGGAVANLSLATSDTWKD
                     KQSGEMKEVTEWHRIVIYGKLAEIAGEYLRKGSKVYIEGQLKTRKWFDQQNGVDRYST
                     EVVVGVNGVMQMLGSLRQQGTAGNGASAPAQGGWGQPPKTAGQAGRAPQTTSTGNGAP
                     PMDFDDDIPFMGLGYGMERRIIHAM"
     gene            13252..14187
                     /locus_tag="ACHKTW_004131"
     CDS             13252..14187
                     /locus_tag="ACHKTW_004131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HFO6465051.1"
                     /translation="MSEKVNKTSLIHDAAFRKTLKDPAAARDFLEQVLTPYQKSRCNL
                     DTIELEPTTFVAESLRQSACDVLLSMKTNDGKDGYIYTLIEHQSSPDKFIPLRMMRYI
                     LAVMEQHIEEHKCAPVVIPVLFYHGAKRPYPYPMNWVDCLDDPVYGREIYGEQKPFSL
                     VDVSTLTDDEIEHYHRMAALMFTMKSGTSGDVIELIGKSITLTDKYGSSVHQNIVLTY
                     LMELYQMDFAELSEAVSTHYPSHKGVIMTIAEQLEEKGLEKGRAEERSRLVLMMRQKG
                     KSLEEIKDFLDLTDEQLLQALDYVPVQRNGALNQD"
BASE COUNT         3645 a         3183 c         4159 g         3364 t
ORIGIN      
        1 ctggatgaga tgacctgtgg cctgcagccc ggggatctta tccttctggc agcacgtccg
       61 tcgatgggga aaacggccct tggactcact ctgtgtctta atgccctgcg tgagcggacc
      121 gatgaaacgg tattcctgtt cagcatagaa atgtccaggg agcagctgat tctgcgtacc
      181 ctgtcgatgc tgtcccgggt ggagctgact cgtcttcgca gcagcatgat ggacgatgag
      241 gagtgggcga gacttacggc aacaatgggt gagttcatgc aggaggggac atttgccggt
      301 gaaggtaacc gcctgatcat tgatgacacg tcatctcaga ctccgtcatc actgcgcgcc
      361 agtgcccgtc gttatgtgcg tctgtatggc agaccttccc tgataatggt cgattatctc
      421 cagttgattc gttcaccgga ccaggaaaac cgcactcagg agattgcgga aatatcccgc
      481 gctctcaagt cgctggggaa agaacttgga tgccctgtgc tcgcactgtc gcagcttaac
      541 cgacaggtgg aacagcgggc agataaacga ccaaacaacg gtgacctgcg tgattccggg
      601 gcgctggagc aggatgctga cctcattatg tttatttacc gtgacgaggt ctacaacccc
      661 ggcactctgg ataagggcgt ggcggaaatc attgtgagca aacagcgaca gggaccgacc
      721 ggtacgatac gggtgagctt cgatggtcgt tacaccctgt tttcaccgtt tgaacgtggc
      781 tggaattttg accgttccgg aggcaatgcg tatgtcaatt aagcgaaatc attcccttga
      841 tctgggggcc gccatcatgc aaccggggcg ccaggccagt aagaccggta acgtggttgc
      901 cctgggggaa acgcccatgg ttctgacgct ggatcagttg cgccctaatc ctgataaccc
      961 gagaaccacg cgcaatcccc ggtacgacga tattaaaagt tccattttct cccgcggact
     1021 ggatacggtg ccaaaggtca cccggatacc ggagagtgag ccggatatct acatcttcag
     1081 cgatggtggt aacactcgtt atcagatcct gagagaactg tgggaggaga ccggcgagca
     1141 gcgtttctac cgtattcatg tgcttttcaa accgtggccg gggcggcttc agtgtgtcat
     1201 tggtcatctg gctgagaatg aagtcagggg tgaactgacg tttattgaaa aggctcaggg
     1261 tatccataaa gcacggatga tttatgaaga gtccctgcag cggactgtga cgatcagaga
     1321 actggcatca ctactgacag atgaagggct tcctgtaaat cattcatccg tcagcagaat
     1381 ggagcatgcg ctgaaatatc tgtatccgtg gatccctgat cttctggagt caggtatggg
     1441 gagtccgcag gtcagagcct tactgtctct ccgtcaggat gcggaacgca tctgggggca
     1501 gtttgcagtc gcggcggaaa acgatgtgga ttttgatcgc gtgtttggtg aatgttgcgg
     1561 caaattcaac tcgccggaac tctggtccat ggaaatgttc agggatgagc tgatcggtga
     1621 cctgctgaag gctctgccac atccggtact ggattacgat cgctggttgc tggagctgga
     1681 tccccgggag cgtaatcgac gtcaccattt tggtgaaccc gaaccggtac tcatgccggc
     1741 ggacagcccc ccagaaccgg gggcgcaggt ggcgtctggc ggggcgacgg aagttgctca
     1801 ggtcgttgcc gccactcccg cagaccgtga gagcgtcatg gatactgcgc aatcaggggc
     1861 tgagaaagag gaccgcccgg tcgcagataa atgcgaacga cgcagaaagg aagttcaacc
     1921 ggatatgtat ggtggcgaag actcatacag cggtgattgc ggagaggatg cgaccggtgc
     1981 gtcaactgat attccgataa tgccggaaga taacgccatg caggaggggg gagaatttcg
     2041 ccaggcgtct gcattcactg gtgctgaaca ggatgatatc gaatctctgc agaatgaggc
     2101 gtttcacctc gcatgggagc tggcgtcaca tacaggctgt gtcgatgaga tagcgatgga
     2161 ccgggaaagt gagctgtctg caggatttgg tgcggcagag gcaaattgct caccggtcat
     2221 ggcatttctt gtcagactga ccggtgagga ggttctgcgg gtcaatccgg tttcccttga
     2281 tgcactgctg ataggtggca gtactgaaga agacctgccg ttgcttgatg atgaacatgc
     2341 aggaaagttg cttcgcctgc tgggtgtgtt gcgtcgtctg cgggggctgc agcgtatggt
     2401 ggaagacgag agtgaggaag ggcgcgatga atgctgatgc cgcacgtcag gatcaactga
     2461 ttaaacggat aagaaaactg ctggcgctga gccggaataa cagtaacccc catgaggcag
     2521 gccaggcact ttcccgtgct caggcgctga tgcgcaagca cggtatcact gaactggata
     2581 ctgatatttc ggtgataaag acctcgtcat caaagggggc cccgtcagaa gcgaaaaaag
     2641 ttccggtatg gatggggcgc cttatgcatg ttgttgcaga ggcattcgga tgccatacat
     2701 acctggactg gcgtatcaca accggaaggt actggcggcg ggttgtcata ttttatggct
     2761 ttggggagcg cccggttgtg gcggcgtatg cctttgatgt tctctgccgg cagatgcaaa
     2821 aggcaacaaa ggcgtatctt tcaacccaga gcaggacatt aaaattgtca acccgcaggg
     2881 caagagcagc catgtttcgt gacggctggg tttatggggt gcgtgatgtg gtggatgcat
     2941 tcccgctcac ggaagatgag acgcgtgtaa tgaagacgtg gctgaagaga cagcaatttg
     3001 agggaatggc tgccttccgg aatgttaagg actgtaatgg tggtgatcgt gcacgctggc
     3061 agggatacag cgagggtaaa aaggcaacac tgcatcatgg tgttggttcc cggaatctga
     3121 gacctgaaag cctgatatcg ggcggaggtg aggtatgagt aacaacttgt cacaggccac
     3181 caacgggctg ttgatgcaac tggtgatgga tctcaagagt ggttatctgc ggcgatgtga
     3241 agcactgggg ctttcccggg aagaaatgca gatgctgcag gggctgagca ttgaagaaat
     3301 tcactatctg tcaaattcag aagtgtcggt gctgcggctg gatattaacc acaacaatct
     3361 tgtgcgcatg ttgcagcagg cgcggactga acagaaacgt ctgcagcgaa tcgatcgcgc
     3421 tctggcgctg gggggctcta tcgaactcat ggcgttttat tttggtcttt ccagcgtgga
     3481 tgtggctgcg cggcgtcgta tctctggtat tgatgttcgt cccggaaggg ggatcaccct
     3541 gagtgacgat gagaattctg aactctggcg gttgtggcag aaagccggga tcaatgatgt
     3601 ggagagtgtg gatgggctgg atgtcatgat gctttgtgct gaacagatga acattccact
     3661 gacggccatc tggcatgccg tcagagggtg gcatacaacc ggaaatccgg atcaggtaag
     3721 gagcgcgtca tgaagatgac agaactggtt cccggagtgc tgacaagtcc tggcgtccct
     3781 cccgccagtc agcgtaaggc ggttgaactg gtcaatcgct tccgtcgtgg agagcggaat
     3841 tatcatgaac tgaaggaaag gggatttggt tatttcaaga ttgatgttgg tcctgactgg
     3901 cggttactga gccggaataa agggctgacc tgggccctgt tgagtcatga acgatacaac
     3961 aacatgagac tgaagtgagg tggaaagatg agtaatgcag caagactcca gctgggtttt
     4021 tctccgtttt caaaaactat cgtgctggca aaaatgcgta atcttgacga cggaaccagt
     4081 cttcgtgtcg gtaatgatcc cggccgcgat gtaaccaatg aggcggctca gcttgtctgg
     4141 catctggtca tggcagaagg tggtgaaatt atgtgggaaa tggacgatgg ctcccgcatg
     4201 gtgctgaagg ctgaaagtca ggaggcaacc agtgaacaag attgactatc aggcactgcg
     4261 taaggcagca caaaactatc agtcgacgct ggcgtggtat cagtccatcc cggacagccc
     4321 aaatgctgaa cgggattgtg atgcggctct ggctgcgttt aagcatcaca tccgtcatcg
     4381 ggaagtggat attatctccg ggctgttgga tgagctggac gaaaaacagc aatacattaa
     4441 atcccgcgac caggagaatg aggatattgc tcttacggtt gggaagctga gagttgagct
     4501 tgaggaagta aaacaacacg ctgaagaatt atccgaaacc aacgctgttc gtaaccaatg
     4561 gcggccagat atttgcccaa taaccggacg tgcatttttc atgtggattg agcatccgac
     4621 attgggaaat gtgccgacgt atggtggccc attagacagt tacaccattc caacaaagga
     4681 cggtgacggt gagttttcat gtgagcgtta cgatcatgat ttcggcggtt gggtagaaag
     4741 cgaatgtcct gggttatatc tgattgatga taaagaacaa tgcagagtct acgaactgga
     4801 ggaacgagtt aaggaactgg atgctcggga aatatcgctc ccggaacgta gcagtatgct
     4861 tcatcgaaca gattttaacg aggcttacca cacggtaatg gcatacaaag tttctgaagt
     4921 catcgctgca atccgcgtcg ctggcattcg catcaaagga gagtgatatg gcgttaacac
     4981 accgcgaact ctgtcagatt gcgtacaagt tccttaagcg caacgggttc aaggtttgct
     5041 ttcatgaccg ctttatagct gtaaccagca ctggagaaca gccagatgct atgggattca
     5101 gaaattcagc atcatgcctg atagaggtga agtgttctcg tgctgatttg ttggcagata
     5161 gaaaaaagcg tttccgtaaa aatccctctc ttggcatggg cgactggcga ttctttatta
     5221 gtgagccggg aattatttca gttgaggatt tacctcctgg ctggggatta cttcacgttg
     5281 ttaacggaag agttcggaaa gtacatggat ggcccagggg taattgctgt tggggtaatc
     5341 ctgacgataa gccatttacc gggaataagc aggttgaatg cgattacatg ttatctgcat
     5401 taaggcggat ggagttgaga gggcacctta atgaaatata tgacggtgta attgttaata
     5461 agaaagaagg aaacgcggca tgatcactat taccaaagga cgactgctga caatcaggca
     5521 gtggtgcgaa acatacggac cgggtagcaa cgttgtactg cctgcagaag aagcggaaga
     5581 actggcgcgg attgccctgg cgtcgctgga agccgagccg gttggtgagt tttatgaata
     5641 caaaccgggt gactggtatc agcgttcggt cggggataaa gcgccaaagt ggacaccact
     5701 ctacgctgtg ccaccagtgc ttgtggcacc tgaaggactg gttaaggctg tgcgctttta
     5761 tgaacaggtt aagcgtgaaa atccgccagt cgaaaccgga gcatggaaag acgctgttga
     5821 ctgggtatta caggaagcat gtcaggctgt tcattggtgc cgacagatat cagaccttca
     5881 ggaacaggag aatcattcat gagaaaaatc agtggaatgt tgaatctgga gaaggccagg
     5941 atgcagttgc gtgttttgtt atctggacct gttcctgcaa tcactccgat ggcgcatgcc
     6001 gtggcaataa aagttgtgct tgatgctctg gatgctagtg agcggagggt ggctgaactg
     6061 gaacaggtct gtactgccgc ggggattcgt atcaaagggg aacaagtgaa atgaaactga
     6121 aaatgcagac aatttcgggt cctgtgatta tcgacagtaa tgaggtgaca cagttttacc
     6181 cggaccatga aagtggaggg gaactcaccg ctgttgaata ctttactgac gagggaagat
     6241 gcaccacccg ggtacgccat tccttttatc aggttgctga ggcacttgcc agtgcatggc
     6301 atgtggatga tgcatcaaaa aggaatcgcc gtggataacc tgaggtgccg gggttatgtc
     6361 agttctgaag ctgctgaacc gtgacataaa ttttttatgt tgcggtgaag cagtcgacaa
     6421 acgaagtgcg tcagcgtgct ggttattaag gcaatatttt ttcagaggaa ccgcaatacc
     6481 gtcggtatta acggtttacg ggtgatgaag tgtttagcct gcatctttgc cgggtgcagt
     6541 tataagcatt tcagtaacag aaccccggtg ttgttttccg ggattgaagg ggaattgaat
     6601 catggcgtta ccagcagaaa gccttattgc gcatacgctc agaaaaatga aggccagcat
     6661 ggcagagaaa gccagtcgtg aggagggcag gatacgcagt gggctgctgt ttaccggtaa
     6721 tgttcatgat gcggtgcccc gtaggctgct gctggacacg cgcctttctc ctcttgataa
     6781 aaccgcatgg atgatgattc gtctgcatgc gctgagtaat gagggcgcag tattccccac
     6841 ctacgatgaa cttcagttac aactggcctc acccggtagc gggaaggcat cccgtgaaac
     6901 cgtaagccgg gtactgacca tgcttcggct gaccggctgg ctgagtctgt gtaaacgggt
     6961 tcgtgatgaa aaaggccgta tccgtggaaa tatctacgcc ctgcatgatg aacctcttgg
     7021 ctgttgtgat gcagaaaagt ttgaccctcg ctacctggat atggtgctgg attctgcatg
     7081 caagaacagg aacaagacca tccggtatac agccagagag gttctcagtg atatcaaaaa
     7141 tgaacctggt atgcggcatt accacagcca tatcacgctg tttgaatcac ggatgggcgc
     7201 agcgcagaca ccgtctcaga tggcagcgcg tcagcgggta aatccggcgg tgaatggccg
     7261 tgcagcgaag agcggttcgg aatccgcaca gccccctgta tgtgcgtcgg taaccgaact
     7321 cagtgctcag gtaaccgaac tctgtactgc ggtaaccgaa cccggagaaa aaacagaggt
     7381 aacgcaacgc agtacggatt ccgaacaacc aggaaaatca gtgggttatc actcagtacg
     7441 gaaaccgaac cactacgtac gtaatattac acacagtatg aaaaaaaata cgtacgtatt
     7501 gccggatgca ctgaaatcac aactcagtgt ggaagacacc ggcatgctga cgaagcaact
     7561 gcaggcgttg ccggaaacgc tggcggaagg tgtgctggac agtctgtcac agatgctcag
     7621 caaaaatacg cttcagaatc ccgttggctg gatgctggct gtgatgaaac gtgcgcgtga
     7681 agggaacttt aactcgccgg atatgagcag aagcggaacg atccagtctc agcctgcccg
     7741 tccgtcatct cagtccggac atggatgtga tgaacgtgaa cgtcgtcgca gggaagtggc
     7801 ttcgccagaa aaagcgagaa atattgtcgc agaattccgt gccagcatac agcgtgctaa
     7861 tgctgcccgt ctggctatgc gtggttaagt gctgatctat aaattgagcg atggtggtac
     7921 tgttaccgtg gtacaaaaaa tgagcagtgg tgccactgcc accacggtat aaaaaatgag
     7981 cgatgatggc actgccacca cggtacaaaa aatgagcgat ggtggcactg ccaccacggt
     8041 acaaaaaacg agcgatggtg gcactggcac cacggtacaa aaaacgagcg atggtggcac
     8101 tgccaccacg gtacaaaaaa taatcgatgg tgccactggc accgcggtat aaaaaatgag
     8161 caatggtggc actgccacca cggtacaaaa aataagcgat ggtggcactg ccaccacggt
     8221 acaaaaaatg agcgatggtg gcactgccac cacggtataa aaaatgagca atggtggtac
     8281 tgccaccacg gtacaaaaaa cgagcgatgg tggtactgcc accacggtac aaaaaacgag
     8341 cgatggtggc actgccacca cggtataaga aacaacctgc attctattgt cgaatacatg
     8401 aaaatggcgt ttgcttgttt taattttgca ctgttcaatt ctgttttctt gttgttgtaa
     8461 ctctggttct ttctgacgat ctcgtactca aggcgtgttt atttctggta ctgagaggag
     8521 gtcatatgac tgatgagaaa aaggctgagc cgggtgtatt gaaaagtact ctgtcaatta
     8581 atcttcatac ttattatgcg attcgtttgt gggaaggtcg caagcctgag cctggcgctg
     8641 ataagtctga gcgtcgtcgc agaatcattg gtatgccagg tgttatttca agggcaaaaa
     8701 tcgcatcaaa agattctgag aatgacaatc cgtgggcaga tatgtttttg ttcaaccttg
     8761 aggaaaaact gagcaaatcc aaacaggaga tcgatttact ggttgaacaa ctgaatgagg
     8821 ttttacggaa tgtgccgggg gatattatta tttctggtgt tgcatcaacc agtcctgtca
     8881 acattggtgt gcacagccga tcgcctctgg ggtataagag cgtctggtta cttgtgggat
     8941 atgatgagtt ggtgctcaaa gcctttcagg cgtttcatta tggtcttatc gcccgggcgc
     9001 gtcgtgatga gctcctgaat gccggtggac atgcggtacg tcagatctgc gcccttgcac
     9061 agagctataa aactgtgccc gcaaccagaa gtgatatttc ttcaggatca caaaaaggaa
     9121 aagaggctat tagccgctac ggtatgcctg atcctgatgt ccttagtgga aagaaacgtt
     9181 cctctttttc tgctcctctt aaataatctg gtaacactat gcgtctctat ttatgcgaaa
     9241 aaccttcaca ggccagtgac attgccagag tgcttggggc tgccagacgc ggtgaaggcc
     9301 tgattgaagg taacgacttt gttgtgacat gggcttttgg tcatttgctg gagcaggctc
     9361 caccggaggc gtacggtgag cagtatggca gtccctggag cattgaggtt ctgccggtta
     9421 ttcctgaaga gtggcagatg gtggtgaagg ataagatcgc caggcagttt aatgttatca
     9481 ggcgtcttct cggtaaggca gatgaagtag tgattgccac cgatgctgat cgggaaggcg
     9541 aaattattgc cagagaactg cttgattatt gtggctatac aggagtagtt cgacgcctct
     9601 ggttatccgc actggatgag agcagtatca gggaagcgct gggcagaata cttccaggag
     9661 agaaaactgc gcgtttatat gaggccggta aggggcgcag ccgggcagac tggctgacgg
     9721 ggatcaacct gacgcgtttg tacacgatga aagccaggga gtgcggcgtg tctggtgttc
     9781 tctcgattgg cagggtgcag actcccacgc ttgcgatggt ggtcagacgg gatgcggaaa
     9841 ttaaaaattt tatcccggta ccttactggc aactgaacct ctggcttgag aaagacaacg
     9901 tgcgttttcg cgcagtctgg gttgcggcag agcagtactg tgatgcggaa aagcggtgcg
     9961 tgaatgtgca ggctgccaca agcgtccagt cgcagtgtcg tcaggccggt gttgctgtag
    10021 tcagagaggt cagcagcaaa agagagaaga cgccgcctcc gctttgtttt tcactcggta
    10081 cactgcagga ggtctgctcg aaaaaatttg gtttaggtgc gcaggccgtt cttgatatag
    10141 cccagtcgtt gtacgaaaaa cacaaggcaa cgacgtatcc ccgtactgac tgtggttatc
    10201 tgccggtatc aatgcataaa gaagtgccgg aggttctggc ggctgtggca aacaccgatc
    10261 cggacattgc ccctgaactg gcaaaactca gtcctgatat tgtttccaga gtctggaatg
    10321 ataagaagat cactgcgcat catgcgatta tccccacccg tcaggcgttt aatattggtg
    10381 ccctgagtga taacgaactc agggtttacc ggttaatccg tcagcattat ctggctcagt
    10441 ttttacccgt gcaggaagcg gacgtcactg aagccacttt tgttgttgcc gggcagttat
    10501 ttggcacgcg aggacgggtg gaagtggtga aaggatggcg tgtttttatt gcagatgcgc
    10561 cgcatgatga agatgagggg ggcgatacag agcagacaat gcctctgcct gttctggcta
    10621 agggggatga atgtcgtctt gttgaagctg aactgaaaga gatgaaaacc acgcctccgc
    10681 gtcactatac ggagggcacg ctcattgctg cgatgaaaaa tgcggcggga atggtgagcg
    10741 atccggcact gaaaaaagtg ttacgcgaga atgccggact gggcacggaa gcaacccgtg
    10801 ctgaaatact ggagacactg ttcacccgta aatatattga gaaacagggt aagcatatcc
    10861 gggcaacaca actgggggca gagttgattg caggattacc tgaggtgctg accagtccgg
    10921 gaaccacggc actgtgggag caggccctgg atgatattgc acagggtaaa ctgtcgctgg
    10981 cgtcatttat ggagaagcag aaacagtggc tgaatcacct cgtctcgggc gcccgacggc
    11041 agcagttgca gattacggca ccagtgacgc cggtatgccc actttgccag ggcagaatgc
    11101 ggaagctgaa aggtaagaac ggagagttct ggagttgcca gcgttacccg gagtgtaaag
    11161 gcgtgatttc agagcagaaa acgtcacaca gtaaacgcgg cggcagaaaa attaaaaagc
    11221 gtggatgatg attacttcct tttttttatt tgttaaagtg catacgcttt gatgcgtacc
    11281 ctgctgacca cagtaaaaag agcgggcagc tctgaggtcc ggtgatccgt ttttcacacc
    11341 gacgggatca caaggcgcat atactccgtg aaaaccgcgt cgggagcgat ccccggagct
    11401 tgatgagata aacttcaggg ctggtaaccc cgggcaccgt ttttacggtg ccctttttat
    11461 tttggtgttg actttctttg tgtgagcttt aaatatgaca tggctaactt gtcgctccgg
    11521 cgaccaccaa tgttccatag tggcctgtga ggaacgcctt cgggtggccc atgtcgctcc
    11581 ggcgaccacc aatgttccat atgacctgtg aggagcgcct tcgggtggtc agttttacac
    11641 ctccgtagcc tgaaaggagc tgtgagtact gaagggatac atccctgacg tacgggcaaa
    11701 tctgcacacc tttgagagca gatgtcaccg ctttttacgt gtgatgaagg ctttttttac
    11761 tgcacagaca gccggaaata cagctgattc cggccccccg acgggaacta tcacacccgt
    11821 caggggcggt ggtgttctcc gtcatatctt tttttatcca tggagaacac atcatgtctg
    11881 caacaaatac ttcttctgca aaagctgaat acttcaatct gaccgtaaaa ggtctggggt
    11941 acctcagtaa tgtacgtcag gttaaccatc agaatggctc gttcctttgc tgcgtgatta
    12001 acgcactgag tggaccaacg gacaatccgt cctatacgcg ttttgatgtt acggttgttg
    12061 gtaaagatgc aaccaccctg atctctcgct gccagaaagc agtggatgag gaccgtaagg
    12121 ttttgcttgg ctttgcgtta agcaacctgg ccaccgatat cttcaccctg aacaagggag
    12181 agcatgccgg tgagcagcgc gtcagtctga aagcccgcct gatcaaggtt gactggatta
    12241 aaataggcca ggagcaggtc tataaggctg aaaaagcgga gtccctgcca ccgcaaaatg
    12301 gcacaactaa acgtcaatac gcagaaaact ctttctgatc gtattttcct cagcctccct
    12361 gatgatgaaa acagaccacc atcagggagg tctgttttct tatcgttttt gaaaacttta
    12421 aggaaacaca tatgaaccgt caaaacggca ttcttgcagc tgcggctggc tatacattac
    12481 ccctggaaat tctcaaaagt acgcgtggat attatattgg cactcagtgt tctgaggggc
    12541 cggtgtcccg tgaatctgaa gagtatttca aaaaacatga tcaggctgaa caggcgctga
    12601 aaaatggcac ctggacacag cgttgtgact ggtaatgagg ggggagtatg gcttcacgtg
    12661 gcgtaaacaa agtcatcctg cttgggcatc tgggacagga tccggaggtc aggtactttc
    12721 ctaacggtgg tggtgcagtg gcgaatctgt cactggcgac cagtgatacc tggaaggata
    12781 aacagagcgg tgaaatgaag gaagtgaccg aatggcaccg cattgttatt tatggaaaac
    12841 tggcagaaat cgccggagag taccttcgta agggctctaa ggtctatatc gaaggtcagc
    12901 tgaaaacccg taagtggttt gaccagcaga acggtgtgga ccgttattca actgaggttg
    12961 ttgtgggagt gaatggtgtc atgcagatgc tgggttcact gcgtcagcag ggcactgccg
    13021 gtaatggtgc ttctgcacct gcacaagggg gctggggaca gccgccgaag acggccggac
    13081 aggctggtcg tgcaccccag acaacatcaa cgggtaatgg tgcaccacca atggactttg
    13141 acgatgatat tccctttatg gggctcgggt atggcatgga acgacgaatt attcatgcca
    13201 tgtaacatca accatggggc gttacgcccc acttttttat ccggaggttc cgtgagtgaa
    13261 aaagttaata aaaccagcct gatacatgat gcggcgttca ggaagacact gaaggaccct
    13321 gcagccgcga gggactttct tgaacaggta ttgacaccgt atcagaaatc ccgctgtaat
    13381 cttgatacaa tagaacttga acccacgacg tttgtggcag aaagtttacg ccagtctgcc
    13441 tgcgatgttt tgctcagcat gaagacaaat gacgggaagg acgggtacat ttatacttta
    13501 atagaacacc agagttcgcc ggataaattt atcccactgc gcatgatgcg ttatatcctg
    13561 gcggtgatgg agcagcatat tgaagagcat aagtgtgcgc cagtagtgat cccggtgctg
    13621 ttttatcatg gtgcaaagcg gccatatccg tatccgatga actgggtgga ttgtctggat
    13681 gacccggtgt acggacgcga gatttatggc gagcaaaagc cattcagcct ggtggatgtc
    13741 agcacgctta ccgatgatga aattgaacac tatcaccgga tggcggcgct gatgttcacg
    13801 atgaagtccg gcaccagtgg tgatgtgatt gagctgattg gtaaatcaat cacactgacg
    13861 gataagtatg gcagcagcgt tcaccagaat atcgtgctga cttatctgat ggaactttac
    13921 cagatggatt ttgctgaact ttctgaagca gtatccacac actatcccag ccacaagggg
    13981 gtgattatga cgatagcaga gcaactggaa gagaaaggtc tggagaaagg tcgtgctgaa
    14041 gagcgctcca gattagtgct catgatgaga caaaagggaa aatctctgga ggaaatcaaa
    14101 gattttctgg atctgacaga tgagcagttg ctgcaggccc tggattacgt cccggtgcag
    14161 cgtaatggtg cgctaaacca ggactgaata accggaagac tccgttccct tttacaccac
    14221 gcctgccttg tgcaggcgtt tttttggtag tttctggcag tgacagatat tgagctgact
    14281 ggtaaatcaa tcacactgac ggataagtat ggcagcagcg ttcacctgaa cacggtgctc
    14341 acttatctgc t
//
LOCUS       DAZFQD010000138        14324 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.138, whole genome shotgun sequence.
ACCESSION   DAZFQD010000138 DAZFQD010000000
VERSION     DAZFQD010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14324
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.138"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(107..517)
                     /gene="ybcV"
                     /locus_tag="ACHKTW_004132"
     CDS             complement(107..517)
                     /gene="ybcV"
                     /locus_tag="ACHKTW_004132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309653.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 family protein"
                     /protein_id="HFO6465052.1"
                     /translation="MAQAAIFKEIFDQVRKDLNCELFYSELKRHNVSLYIYYLATDNI
                     HIVLENDNIVLVKGLKKVVNVKFSRNKHLIETSYNKLKSKEITFQQYRENLAKAGVFR
                     WVTNIQEHQRYYYAFDNSLLFTESIQKTTQILPR"
     gene            818..1024
                     /locus_tag="ACHKTW_004133"
     CDS             818..1024
                     /locus_tag="ACHKTW_004133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6465053.1"
                     /translation="MNKEQSADELSLDLIRVKNMLNSTISMSYPDVVIACIEHQVSLE
                     AFRAIEAALVKHDKNSKDYSLVVD"
     gene            1578..2072
                     /locus_tag="ACHKTW_004134"
     CDS             1578..2072
                     /locus_tag="ACHKTW_004134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1441 family protein"
                     /protein_id="HFO6465054.1"
                     /translation="MDRELKNLTLNISQLAALSGVHRQTAAARLQNLPVAGGHESNLK
                     LYRVVDIVSAFLALPPPVAEGEMDAHERKAWYQSERERLKFEQETAQLIPASDVRREF
                     AIWAKAVVQVLETLPDILERDCGLQPAAVSRVQSIIDDLRDQIALRVTEAGADDEEEL
                     QQEE"
     gene            2072..4174
                     /locus_tag="ACHKTW_004135"
     CDS             2072..4174
                     /locus_tag="ACHKTW_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFO6465055.1"
                     /translation="MLNQETAKAARTDSGYILRAPRRMRVADAVAQYMRVPMGAGNSV
                     PWDPLVAPYVIEPMNCLASREYDAVIFVGPARTGKTIGLIDGWVIYNVICDPADMLII
                     QMTEEKAREHSKKRLARTFRVSPEVVSRLSPNKNDNNVYDRTFLAGNYLKIGWPSVNI
                     MSSSDYKCVALTDYDRFPEDIDGEGDAFSLASKRTTTFMSSGMTLVESSPGRDVKDVK
                     WRRTSPHEAPPTTGILSLYNRGDRRRWYWPCPHCGEYFQPCGDVVAGFRDIADPVLAS
                     EAAYIQCPSCSGRILPEQKRELNGRGVWLRDGESINADGSRYGDPRRSRIASFWMEGP
                     AAAYQTLSQLVYKLLTAEQEYETTGSEETLKTVINTDWGLPYLPRASMEQRKSELLEQ
                     RAEPVPSRSVPDGVNFLVATVDVQAGRHRRFVVQVTGYGSRGERWIIDRYNITQSLRG
                     DSDGESQRIDPASYPEDWDVLLTDVFHKSWPLASDPSQQMRLMAMAVDSGGEDGVTDN
                     AYKFWRRCRRDGLGKRIYLFKGDSIRRAKLITRTFPDNTGRTGRRAQAAGDVPLWLLQ
                     TDALKDRVNNALWRDSPGPGYVHFPDWLGSWFYDELTYEERSSDGKWSKPGRGANEAF
                     DLMVYAEALVILHGYEKIRWPDAPEWASRETWLECVPDSTEPSPTPEPVSTPVKKQKR
                     KKTVTDDVNPWLTSGGWL"
     gene            4171..4383
                     /locus_tag="ACHKTW_004136"
     CDS             4171..4383
                     /locus_tag="ACHKTW_004136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6465056.1"
                     /translation="MNQNDIEAMIQRYTEAEMAVLDGKSVTFNGQQMTMENLSEIRQG
                     RQEWERRLAALITRRRGHPGYRLARF"
     gene            4311..5891
                     /locus_tag="ACHKTW_004137"
     CDS             4311..5891
                     /locus_tag="ACHKTW_004137"
                     /inference="COORDINATES: protein motif:HMM:TIGR01539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO6465057.1"
                     /translation="MGAPPCGSDYTTTGASRVPAGEVLMAILDDVIGVFSPGWKAARL
                     RSRAVIQAYEAVKTTRTHKARRENRTADQLSQYGAVSLREQARYLDNNHDLVIGVFDK
                     LEERVVGKNGIIVEPHPVLRNGAIARDLAAEIRTRWSEWSVSPEVTGQFTRPMLERLM
                     LRTWLRDGEVFAQMVSGRINSLTPSAGVHFWLEALEPDFIPMTSDESNRLNQGVFVDD
                     WGRPEKYLVYKSRPVSGRQMETKEVDAERMLHLKFVRRLHQMRGTSLLSGVLIRLSAL
                     KEYEDSELTAARIAAALGMYIRKGDGQSYETDGNDSKENERELTIQPGIIYDDLKPGE
                     EIGMVKSDRPNPNLETFRNGQLRAVAAGSRLSFSSTARNYNGTYSAQRQELVESTDGY
                     LILQDWFIGAVTRPMYRAWLKQAVASGVIRLPRDLDRSSLYTAVYSGPVMPWIDPVKE
                     AEAWKIQIRGGAATESDWVRAGGRNPDDVKRRRKAEIDENRKLDLVFDTDPASDKGGS
                     SAATKRQEPQHTDDQSEE"
     gene            5836..7863
                     /locus_tag="ACHKTW_004138"
     CDS             5836..7863
                     /locus_tag="ACHKTW_004138"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308856.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ClpP-like prohead protease/major capsid protein
                     fusion protein"
                     /protein_id="HFO6465058.1"
                     /translation="MPQRNDRSRSTPTTSPKNNSWFRMQAGHQSDADIYIYDEIGFWG
                     VTAKQFISDLNALGDITHINLHINSPGGDVFEGIAIFNALKTHGASITVYVDGVAASM
                     ASVIAMVGNPVIMPENTFMMIHKPFGFTGGDAEDMRTYADLLDKVEAVLLPAYAQKTG
                     KTTDEIAAMLADETWMSGAECLAHGFADQVTPAVKAMACIQSKRTEEFKKMPESIRNM
                     ITPPRNSAPRVQDDEPAASRTPVQAAAPVVDENSIRAQVLAEQKARVNGINDLFAMFG
                     GRYQTLQAQCLADPECSLEQAREKLLNEMGRESTPSNKNTPAHIYAGNGNFVGDGIRQ
                     ALMARAGFEKTERDNVYNGMTLREYARMSLTERGIGVSSYNPMQMVGAAFTHSTSDFG
                     NILLDVANKAILQGWEDAPETYEQWTRKGQLSDFKIAHRVGMGGFSALRQVREGAEYK
                     YVTTGDKQATIALATYGELFSITRQAIINDDLNMLTDVPMKLGRAAKSTIADLVYAIL
                     TSNPKISTDNVSLFDKAKHANVLESAAMDVASLDKARQLMRVQKEGERHLNIRPAFVL
                     VPTAMESVANQVIRSSSVKGADINAGIINPVKDFATVIAEPRLDDNSQTTFYLAASKG
                     SDTIEVAYLNGVDTPYIDQMEGFSVDGVTTKVRIDAGVAPVDHRGLVKCTA"
     gene            7950..8273
                     /locus_tag="ACHKTW_004139"
     CDS             7950..8273
                     /locus_tag="ACHKTW_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2190 family protein"
                     /protein_id="HFO6465059.1"
                     /translation="MAKNFVEEGKTVAIVASAAISSGDLVQVGDVFAVALTDIPQGET
                     GDGMTEGVFILPKLKTDDMKTGKKVYLKSGKVQLTNSGSDPLVGVVWADAGTSAEEVP
                     VKLNV"
     gene            8266..8541
                     /locus_tag="ACHKTW_004140"
     CDS             8266..8541
                     /locus_tag="ACHKTW_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA breaking-rejoining protein"
                     /protein_id="HFO6465060.1"
                     /translation="MSDPFSRLAARMDAITVRKMGKTASINDVDMAVIPGETLAELNA
                     LSGPAVSLVVFSSGYRPRRGDRVVYDGQQWTVTRHERFNGKPMIFIE"
     gene            8553..9131
                     /locus_tag="ACHKTW_004141"
     CDS             8553..9131
                     /locus_tag="ACHKTW_004141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO6465061.1"
                     /translation="MKGLENAIRNLNSLDTRMVPQASAWAINRVAQKAVSVATRQVAG
                     NTVAGDNQVKGIPLKLVRQRVRVFKASPSGKMTARIRVNRGNLPAIKLGTARVRLARR
                     GGKLQYRGSVLKVGKYLFRDAFIQQLANGRWHVMRRIDGKNRYPIDVVKIPLSGPLTQ
                     AFEDARDHIIAAEMPKQLGYALKQQLRLWLTR"
     gene            9128..9529
                     /locus_tag="ACHKTW_004142"
     CDS             9128..9529
                     /locus_tag="ACHKTW_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFO6465062.1"
                     /translation="MNRHTQIRQAVLARLREQCGDSATFFDGLPAFIDAQELPAVAVW
                     LSDAQYTGKMTDEDDWQAVLHIAVFIRAQAPDSELDMWMESTIFPALNDIPALSGLID
                     TLIPLGFNYQRDNEMATWAMAEITYQITYTN"
     gene            9540..10283
                     /locus_tag="ACHKTW_004143"
     CDS             9540..10283
                     /locus_tag="ACHKTW_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO6465063.1"
                     /translation="MTTPNPLAKTKGAGTTFWMYTGNGDAFANPLSDTDWLRLAMVKD
                     LQPGEMTADAEDDTYLDDEDADWKTTTQGQKSVGDTSATLAWRPGDSGQKKLVQLFDS
                     GEVCAFRIKYPNGTVDVFRGWLSSLGKTIASKDVMTRTVKISGVGRPYLAEEGTETVS
                     VTGLTVSPASASVKVGATTTLTFTVKPDGASDKAISVHSSDPQTATVTLNGLVATVKG
                     VKQGSVSIVGMTADGNFVAVTAVTVSAAG"
     gene            10344..10730
                     /locus_tag="ACHKTW_004144"
     CDS             10344..10730
                     /locus_tag="ACHKTW_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFO6465064.1"
                     /translation="MFLKQDTFNYEKQSVVLSELSGLQRIEYLTFVQQRTAKFDAGEG
                     ELPEAERQIAFLRMGMDINAWLVSRSLWNADQSKDVETLCASVITTWSYDALGAGAEM
                     VLSLSGMGAIDNAVDDEHEALTPEKS"
     gene            10739..11068
                     /locus_tag="ACHKTW_004145"
     CDS             10739..11068
                     /locus_tag="ACHKTW_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFO6465065.1"
                     /translation="MQFVMRLAREFRRADWRRMLSEMSATELGEWGDYFRMQSFSDVW
                     MDAQFASLKALIVRMVSGSSDAAVADFSLLPEENGIPERTDEELMHLGEGISGGVRYG
                     PDSQPGH"
     gene            11040..14105
                     /locus_tag="ACHKTW_004146"
     CDS             11040..14105
                     /locus_tag="ACHKTW_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO6465066.1"
                     /translation="MDQIANLVIDLGIDAAEFKNEIPRIKNLLNGAASDAERSSARMQ
                     RFMERQTQAARQTTQAASSAATAASVHAQTVEKNAQAHERMAREVEKTRQRMEALSQK
                     MREEQAQAMALAEVQDKAAAAFYRQIDSVKQASAGLQELQRIQQQIRQARNSGGIGQQ
                     DYLALISEVTAKTRVLTQAEEEATRQKVAFIRQLKEQATRLNLSSSELLRAKAAQLGV
                     SSAAEVYIRKMEQAGKATHSLGLKSASARQEIGVLIGELARGNLGALRGSGITLANRA
                     GWIDTLMSPKGMMLGGVIGGIAAAVYGLGKAWYDGQKEGEEFNRQLSLTGHYAGVTAG
                     QLWTLSRAISGNGITQHAAAGALAQVVGSGAFRGNDIGMVARAAAQMERSVGQSVSDT
                     INQFKRLKDDPVNAAKALDNELHFLTATQLEQIRVLGEQGRSSDAARIAMSALAEETG
                     RRTADIDNNLNALGSTLKYLSDLWSRFWDAAMNIGREDSLDEQIAALQEKVSRAKRLP
                     WTASSSQVEYDQQRLNDLQEKKRQKDLQDAKEQAERNYQEQQKRRNAENAALNRMNET
                     EAARHQREIARINAMQYADQAVRDAAIQRENERYEKALASGKKKTRETRNDEATRLLL
                     QYSQQQAQVEGQIAAARQSAGIATERMTEAHKQLLALQQRISDLDGKKLTADEKSVLA
                     RKDELIQALTLLDVKQQELQKQTALNELKKKTIQLTSQLAEEERAQRQQHDLDIATVG
                     MGDQQRQRYQVQLSLRQKYQQQLEQLRRDSEQKGTYNTDDYRKAEQALTESLNRQLNE
                     NRRYWQQLEVVQGNWKNGVLRAFQDFTVDADNTAETAEQVFSSAFSNMGNGLATFVTT
                     GKLNFKSFTSSVLSDMAKILAQATMMKSIKGIGSVLGFDLSSLSLNANGGIYQSADLS
                     RYSGTVVNRPTFFAFAKGAGVMGEAGPEAILPLRRGADGKLGVVADIGGSGMAMFSPQ
                     YNIEINNDGTNGQIGPAALKAVYDLGKKAAADFMQQQARDGGRLSGAYR"
     gene            14105..>14324
                     /locus_tag="ACHKTW_004147"
     CDS             14105..>14324
                     /locus_tag="ACHKTW_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO6465067.1"
                     /translation="METFHWKVRPDMNVVSEPKVVTVKLGDGYEQRRAAGLNNQLSTY
                     SVTIRVRKCEHPSLKAFLERHGGVRAFQW"
BASE COUNT         3535 a         3232 c         4207 g         3350 t
ORIGIN      
        1 ttttgttata tttgaatggt tccgaatatc taaatcacaa ttgttgatgg tttttattaa
       61 accaatgcag tccggctcag gagtgagaga agccggacgt tatggtttag cgtggtaaga
      121 tctgtgtagt tttctggatg ctttcagtaa atagtaatga gttatcaaag gcatagtaat
      181 atctttggtg ttcctggata tttgtaaccc atcggaaaac tcctgcttta gcaagatttt
      241 ccctgtattg ttgaaatgtg atttcttttg atttcaactt attataggag gtctctataa
      301 gatgtttgtt tctggagaat ttaacattta caaccttttt gagtcctttt actaacacta
      361 tgttgtcgtt ttctaacaca atgtgaatat tatctgtggc taaatagtaa atataaagtg
      421 agacattgtg acgttttagc tcagaataaa ataattcaca gtttaaatct ttacgcactt
      481 gatcgaatat ttctttaaaa atggcagcct gagccattgg taaaccttcc atgtgatacg
      541 atagcgcgta gtttgcatta tcgtgtttat tgtttcaatc tggtctgacc tctttgtgtt
      601 ttgttgatga tttatgtcaa atattaagcc tgtttttaat gaatagtgtt gattgcgtaa
      661 caaagttgag ccttgctggc atccaggagg gatatgcaac cgacagatgt atgtaaggtc
      721 gatgtactca aactttcata cttttcctct tttatgcaga aagatttgaa gtaatatttt
      781 aaccgctaga tgacgagcaa acgcatggag cgacaaaatg aataaagaac aatctgctga
      841 tgaactctcg ttggatctga ttcgtgtaaa aaatatgctt aatagcacca tttctatgag
      901 ttacccggat gttgtaattg catgtataga acatcaggtg tctctggaag cattcagggc
      961 aattgaggca gcgttggtga agcacgataa gaattcgaag gattattccc tggtggttga
     1021 ctgagcacca taactgctaa tcattcaaac tatttcacct gtgacagagt caatgtcgca
     1081 ttctgtcact gtcatgctaa tacagagctg caattcaact actgcaatgt cctcgtaatt
     1141 aggtgaattt acaatatcgt cctgttcgga tgccggctgc attgctgaag atgaggcatt
     1201 tatggttcgc atattttccc ctcatgctcg tcagtcctgt gcgtaggaag aaacaggaca
     1261 ctcacactaa tttgtgtggg catgctgtga tgtccttctg aattattcct atgccattat
     1321 gtaaagcgct gtatcagatg ctcgtcacgg ctgtcaggct gtcgggtcct cccggtgggg
     1381 cccctgccac ggggcgggag cgtcgcggaa aaaggctagt ttttgaaatt ttattcgtca
     1441 tcaccactac tgtaattgat tgatattaca gttgttttat ttttatggtg tcgattctga
     1501 ttgttttttg ttcatcacta acaccgtttg cctaaagttg ttcgcaagat gcatgtttaa
     1561 aacattctgg agcgggtatg gatcgagagt taaaaaatct gacgctgaat atcagtcaac
     1621 tggcggcatt gtcaggtgta catcgccaga ctgctgcggc aaggctgcaa aatctacccg
     1681 ttgcaggggg gcatgaaagc aacctcaagc tttatcgggt ggttgatatt gtgtcggcat
     1741 ttctggcatt accaccgccg gttgcagaag gcgaaatgga cgcgcatgag cgcaaagcct
     1801 ggtatcagtc tgaacgtgag cgtcttaagt tcgaacagga aacggcacaa ctcattccgg
     1861 ccagtgatgt cagacgggag tttgccatct gggcaaaagc ggtcgtgcag gtgctggaga
     1921 cattaccgga tattcttgaa cgtgactgcg gtctgcagcc tgccgctgtg agccgtgttc
     1981 agtccattat tgatgatctg cgcgatcaga tagccctgcg ggtgactgaa gcaggtgcgg
     2041 atgatgagga ggaattacag caggaggagt aatgctgaat caggaaaccg caaaggcagc
     2101 acgaaccgat tcaggttata tccttcgcgc accgagacga atgcgggttg ctgatgccgt
     2161 tgctcagtat atgcgggtgc ccatgggggc agggaactca gtcccgtggg atccgctggt
     2221 ggcaccgtat gttattgagc ctatgaactg cctggcctcg cgtgaatacg acgcagtgat
     2281 atttgttggc ccggcacgaa ccggcaagac tatcggcctg attgacggct gggtgattta
     2341 caacgtgatt tgcgatcctg ctgatatgct gatcattcag atgacggagg aaaaagcccg
     2401 cgaacactcc aaaaaacgac tcgccagaac gtttcgcgtc agcccggaag tggtcagtcg
     2461 cctgagtccg aacaaaaatg acaacaacgt ttatgacaga acattccttg ctggcaacta
     2521 cctgaaaatc ggctggccgt cagtcaatat catgtcttca tcagattata aatgcgtggc
     2581 gctgacggat tatgaccgtt ttccggaaga tattgatggt gagggggatg ccttctctct
     2641 tgcctcaaaa cgtaccacca catttatgtc cagtggtatg acgctggtgg agagttcccc
     2701 cggcagggat gtgaaggatg tgaaatggcg acggacttca ccgcatgagg ctccaccaac
     2761 cacggggatc ctgtcgctct ataaccgtgg cgatcgccgt cgctggtact ggccctgtcc
     2821 acactgtggt gagtattttc agccctgcgg cgatgtggtt gctggtttcc gtgatattgc
     2881 cgatcctgtg ctggcaagtg aggcggctta tattcagtgt ccttcctgtt caggacggat
     2941 tttgcctgaa caaaaacgcg agctgaacgg acgtggggtc tggttacggg atggtgaatc
     3001 catcaatgca gatggcagtc gttatggtga tccccggcgt tcacgtattg cgtcattctg
     3061 gatggagggg ccggcagctg cttatcagac actctcgcaa ctcgtttaca aactgcttac
     3121 tgcagaacag gaatacgaga caaccggaag tgaagagaca ctcaagacgg ttatcaatac
     3181 cgactgggga ttaccttatc ttccccgcgc cagcatggag caacgaaaaa gtgaattgct
     3241 tgagcagcgg gcagagccag ttccttcccg cagtgtgccg gatggcgtta atttccttgt
     3301 ggcgacagtg gatgtgcagg cgggacgtca tcgccgtttt gtggttcagg taacgggcta
     3361 tggcagccgt ggcgaacgct ggattattga tcgttacaac atcacgcagt cattgcgcgg
     3421 tgacagcgac ggggagagcc agcgaattga tccggccagc tatccggaag actgggatgt
     3481 cctgctgacg gatgtttttc ataaaagctg gccgctggcc tccgatcctt ctcaacaaat
     3541 gcgactgatg gcaatggcgg tggactccgg cggtgaagac ggggtcactg ataatgccta
     3601 taaattctgg cgtcgttgcc gtcgtgatgg ccttggtaaa cgtatttacc tgtttaaggg
     3661 cgacagcatc cggcgcgcaa aactgatcac ccgtacattc cctgataaca ccggacgaac
     3721 gggccgacgg gcgcaggccg caggtgatgt accgctctgg cttcttcaga cggatgccct
     3781 gaaagaccgg gtgaataacg cgttatggcg tgactcgcca ggtcccggct atgtgcattt
     3841 ccctgactgg ctggggagct ggttttacga cgaactgacg tatgaagagc ggagcagtga
     3901 cgggaaatgg agtaagccgg gtcgcggtgc caacgaagct tttgacctga tggtgtatgc
     3961 cgaggctctg gtcattctgc atggatacga aaagatccgc tggccggatg caccggagtg
     4021 ggcgagccgg gaaacctggc tggagtgtgt cccggacagt accgaaccgt cacccacacc
     4081 ggaaccggta tccacgcctg ttaaaaaaca aaaacggaag aaaacagtaa ctgacgatgt
     4141 taacccctgg ctgacttccg gaggatggtt atgaatcaga atgatattga agccatgatt
     4201 cagcgttata cggaagctga aatggcagtg ctggacggaa aatccgtcac ttttaatggt
     4261 cagcagatga ccatggaaaa cttatctgag atccggcagg ggcggcagga gtgggagcgc
     4321 cgccttgcgg ctctgattac acgacgacgg gggcatcccg ggtaccggct ggcgaggttc
     4381 tgatggcaat tcttgatgat gtgattggcg ttttttcacc aggatggaaa gcggcaaggc
     4441 tgcgttcccg agcggtgatc caagcttatg aggccgtaaa aacgacgcgg acacacaaag
     4501 cccgacggga gaaccgaact gccgaccagt taagccagta cggggccgtg tcgttacgtg
     4561 agcaggcccg ttaccttgat aacaaccacg atctggtcat tggtgtattt gacaagctgg
     4621 aagaacgggt ggtggggaaa aacgggatta ttgtcgagcc acatccggta ttacgcaatg
     4681 gtgccattgc ccgtgacctg gcagcggaga tacgtacccg atggagtgaa tggtctgtca
     4741 gtccggaagt caccgggcag tttacccgtc cgatgctgga acgtctgatg ctgcgtacct
     4801 ggctgcgcga tggtgaggtg tttgcccaga tggtttccgg gcgcataaac agcctgacgc
     4861 cttctgccgg tgttcatttc tggctggagg cgctcgagcc ggactttatt cccatgacca
     4921 gtgatgagag caacaggctg aatcagggcg tgtttgttga tgactggggg cgtcccgaaa
     4981 aatatctggt gtataaaagt cgtcctgtat ccggacggca gatggaaacc aaagaagtgg
     5041 atgcagagcg aatgctgcat cttaaatttg ttcgccgtct gcaccagatg cgcgggacgt
     5101 ctttattgtc cggtgtgctg atccgcctca gcgccctgaa agagtatgaa gattctgagc
     5161 tgactgcagc aaggatcgcc gctgctctgg ggatgtacat ccggaaaggc gacggacaga
     5221 gctatgaaac ggatggtaat gacagcaagg agaatgaacg cgagcttacc attcagccag
     5281 gcattattta cgacgatctg aaacccggcg aagaaatcgg aatggtgaag tcggatcgtc
     5341 ccaatcctaa ccttgaaact tttcgtaatg gtcagttgcg tgccgtggcg gcgggcagtc
     5401 gtctgagttt ttccagtacg gcacgcaact ataacggcac ttacagcgcc cagcgtcagg
     5461 aactggttga gtctactgat ggctacctga tcctgcagga ctggtttatt ggtgccgtca
     5521 cccgcccgat gtatcgtgcc tggctgaaac aggctgtggc atccggtgtt atcaggctac
     5581 cccgcgatct tgaccgttct tcactgtata ccgcggtgta ttccggacca gtgatgccgt
     5641 ggattgaccc tgttaaggag gctgaggcct ggaaaatcca gattcgtggt ggagcggcga
     5701 cagaatcaga ctgggtacgt gctggtggtc gtaatccgga tgatgtcaaa cgtcggcgca
     5761 aggccgaaat tgatgaaaac cgcaagctgg atctggtatt tgataccgat ccggccagtg
     5821 ataaaggagg cagcagtgcc gcaacgaaac gacaggagcc gcagcacacc gacgaccagt
     5881 ccgaagaata attcctggtt caggatgcag gctggtcacc agagtgacgc ggatatttat
     5941 atttatgacg agattggttt ctggggtgtt acagcgaagc agtttatcag tgatctgaat
     6001 gcactgggcg atatcaccca cattaatctc catattaatt caccgggtgg cgatgtcttt
     6061 gaaggcatcg ccatttttaa tgcgctgaaa acacatggtg cgtccattac cgtttatgtc
     6121 gacggtgtgg cggcgtcaat ggcgtcggtc attgcgatgg tgggaaaccc ggtcattatg
     6181 ccggaaaaca ctttcatgat gattcataaa ccatttggct ttacgggcgg tgatgcggag
     6241 gacatgcgca cctatgccga cctgctcgat aaagttgagg cggttctgtt acccgcttat
     6301 gcacagaaaa ccgggaaaac caccgatgaa attgctgcca tgctggcgga tgagacctgg
     6361 atgtccggtg ccgaatgtct ggcacatgga tttgctgatc aggtaacgcc agccgttaag
     6421 gcaatggcat gtattcagtc aaaacgtaca gaggaattta aaaagatgcc ggaatccatt
     6481 cgaaacatga ttactccgcc acgcaacagt gctccacgcg tacaggatga tgaacctgca
     6541 gcctcccgga cgccagtgca ggcagcagca cccgtggtgg atgaaaacag tatccgtgcg
     6601 caggtactgg cagagcaaaa agcgcgtgta aacggtatta atgatctgtt tgccatgttt
     6661 ggcgggcgtt atcagactct gcaggctcag tgtcttgccg atcctgaatg ttcgctggag
     6721 caggcccgcg aaaagctgtt gaacgagatg gggcgcgagt ccacgccatc caataaaaat
     6781 accccggctc atatttatgc cggtaacggt aattttgtgg gggacgggat ccgccaggcg
     6841 ctgatggcgc gtgccggatt tgaaaaaacc gaacgtgata atgtctacaa cgggatgacc
     6901 ctgcgtgaat atgcccgtat gtcactgact gaacggggta ttggggtttc cagttataac
     6961 ccgatgcaga tggtcggtgc ggcgttcaca cacagtacgt ctgacttcgg taatattctg
     7021 ctggatgttg cgaacaaagc cattctgcag ggctgggaag atgctcctga aacctatgaa
     7081 cagtggacgc ggaaaggtca gttgtctgat tttaaaattg cccatcgtgt gggtatgggg
     7141 ggcttcagtg ctctgcgtca ggtgcgtgaa ggggcggaat ataaatacgt caccaccgga
     7201 gataaacagg ccactattgc actggcgacc tatggcgagc tgttcagtat cacccgtcag
     7261 gccattatca atgatgatct gaatatgctg accgatgtcc cgatgaagct gggccgtgcg
     7321 gcgaaatcca ccattgccga tctggtttat gccattctga cgtctaaccc gaaaatctcc
     7381 acagataatg taagtctgtt cgataaagcg aaacatgcaa acgtactgga gagcgctgca
     7441 atggacgtgg catcgctgga taaagcccgc cagttgatgc gcgttcagaa agagggggag
     7501 cgtcatctga atattcgtcc tgcgttcgta ctggtaccga cggcgatgga gtctgttgct
     7561 aaccaggtca ttcgctcctc aagtgtcaag ggggctgaca ttaacgcagg tattattaac
     7621 ccggtgaaag attttgcgac cgttattgca gagcctcgtc ttgatgataa cagccagacc
     7681 accttctacc tggctgcgtc caaaggctcc gatacgattg aagtggctta tctcaacggt
     7741 gtggatacgc catatattga tcagatggag ggcttcagtg tggatggcgt gacaacgaaa
     7801 gtgcgtattg acgccggtgt cgcgccagtt gatcaccgcg gtctggtgaa atgtacggcg
     7861 taaacgtcgc agacaacaac tctgatggcc cgtaagggct ttttttgtac ctgaaatcag
     7921 cctctgaacg gggctgtacg gagacagtta tggcaaagaa ttttgtagaa gaaggaaaaa
     7981 cggtggcgat tgttgccagt gcagccatca gcagcggaga tctggtgcag gtgggtgatg
     8041 tttttgcggt ggcgctgacc gatattccac agggtgaaac aggcgacggc atgaccgaag
     8101 gtgtgtttat cctgcctaaa ctgaaaacgg atgacatgaa aacgggtaag aaggtttatc
     8161 tgaagtccgg aaaagttcag ctgactaaca gcggctctga tccgctggtc ggggttgtct
     8221 gggcagatgc cggaaccagt gcagaagaag tgccggtaaa actcaatgtc tgatcccttt
     8281 tcccggctgg cagcgcgtat ggatgcgatc acggtcagaa agatgggaaa gacagcctcg
     8341 attaatgatg tcgatatggc tgtgatcccg ggcgaaacac tggcagagct gaatgctctg
     8401 tccggacctg cggtctctct ggtggtgttt tcttcgggat accgcccacg gcgcggggat
     8461 cgcgttgttt atgacggaca acaatggacg gtcacacggc atgaacgttt taacggtaag
     8521 ccaatgatct ttattgagta aagaggtgtg ggatgaaggg gcttgagaat gccatccgca
     8581 atctgaacag ccttgatacc cgtatggtgc cacaggccag cgcatgggcg ataaaccgtg
     8641 tggcacagaa agcggtctcg gttgccaccc ggcaggttgc cgggaatacc gttgcgggag
     8701 ataaccaggt gaaagggatc cccctgaaac tggtacgtca gcgtgtccgg gtgtttaaag
     8761 ccagtccgtc aggaaaaatg acggccagga tccgcgttaa ccggggcaat ctgcccgcca
     8821 ttaagctggg gacagcccgg gtcagactgg cccggcgtgg tggaaaactg cagtaccgtg
     8881 gcagtgtgct gaaggtgggt aaatatcttt tccgggatgc gtttattcag caactggcga
     8941 atggtcgctg gcatgtgatg cggcgtattg atggcaaaaa tcgttacccc attgatgtgg
     9001 tgaaaatccc gctgtccgga ccgctgacac aggcatttga agatgcccgc gaccacatca
     9061 ttgctgcgga aatgccgaaa cagctggggt atgcactgaa acaacaactg aggttatggc
     9121 tgacccgatg aaccggcata cacaaatccg ccaggccgta ctggcacgcc ttcgggaaca
     9181 gtgtggagac agcgccacgt tttttgacgg gcttccggca tttattgatg cgcaggaact
     9241 gcctgccgtg gcggtgtggc tgagtgatgc tcagtacacc ggaaaaatga cggatgaaga
     9301 tgactggcag gctgttctgc atattgccgt cttcatccgg gcacaggcac cggattcaga
     9361 gctggatatg tggatggaga gcaccatttt ccctgccctg aatgatatac cggcactttc
     9421 cggactcatc gacaccctga tcccactcgg ttttaactat caacgtgata atgagatggc
     9481 cacctgggcg atggcggaaa tcacgtacca gatcacgtac acgaattaag gaggtggtaa
     9541 tgaccacacc aaatccactg gcaaagacga aaggtgcggg gacgacgttc tggatgtata
     9601 ccggcaacgg cgatgcgttt gcgaaccctt tgtcggacac tgactggctg cgtcttgcga
     9661 tggtgaagga tctgcaacct ggcgaaatga ccgctgatgc agaagatgac acttatctcg
     9721 atgatgaaga tgcagactgg aaaacgacaa cccaggggca gaaatccgtt ggtgatactt
     9781 cggcgacgct ggcctggcgt ccgggtgaca gcgggcagaa aaaactggtt cagttgttcg
     9841 actccggtga agtctgcgcg tttcgtatca aatatcccaa cggcactgtt gatgttttcc
     9901 gtggctggct gagctcactg ggtaaaacca ttgcctcaaa agacgtgatg acccgcactg
     9961 tgaaaatcag cggtgtgggg cgtccgtatc tggcagagga aggcactgaa accgtgagcg
    10021 ttaccgggct gacagtatca ccggcgtctg ccagtgtaaa agtgggagca accaccacgc
    10081 tgacctttac agtaaaacct gacggagcca gtgacaaagc gatcagtgtg cattcgtcag
    10141 atccacagac tgccacggtt accctgaacg gacttgtggc cacggtaaaa ggcgtgaagc
    10201 agggcagtgt cagcattgtg ggcatgaccg ctgacgggaa ttttgtggct gtgactgcgg
    10261 tgactgtcag cgcagcaggt taacaggacg atactcatca tttgccccgg ttatgcgggg
    10321 cttttttgca ggtggagaac atgatgtttc tgaaacagga cacgtttaat tatgaaaaac
    10381 agtccgtggt gctcagtgag ctgtccgggc tgcagagaat tgaatatctg acgtttgttc
    10441 agcagcgaac ggcaaagttt gatgccgggg agggagaact gccggaggct gaacgacaga
    10501 ttgcttttct gcggatgggg atggatatca atgcctggct ggtttcccgc tcactgtgga
    10561 atgcggatca gtctaaggat gtagagacgc tttgcgcatc cgttattaca acatggtcgt
    10621 atgatgccct gggtgcgggg gcggagatgg ttctgtcgct gagcggtatg ggggccattg
    10681 ataatgccgt ggatgatgag catgaggcgc tgacgccgga aaagtcctga cgcgggaaat
    10741 gcagtttgtc atgcggcttg cccgggagtt ccggcgggca gactggcggc ggatgctgtc
    10801 ggaaatgtcg gccactgagc ttggtgagtg gggcgattat ttccggatgc agagcttcag
    10861 tgatgtgtgg atggatgcgc agtttgcctc gctgaaggca ttgatcgtga gaatggtgtc
    10921 cggcagcagt gatgctgcgg tggctgattt cagcctttta ccggaagaga acgggatacc
    10981 ggagcgaacg gacgaagaac tgatgcatct tggggaaggt atttccggag gtgtgcgtta
    11041 tggaccagat agccaacctg gtcattgatt tggggattga tgcggcagag tttaaaaatg
    11101 aaattccccg tatcaaaaac cttctgaatg gtgcagccag cgatgcagaa cggtcttctg
    11161 cccgtatgca gcgttttatg gagcgtcaga ctcaggccgc ccggcagaca acgcaggcag
    11221 cttcttcggc tgcaacagcc gcatccgtcc atgcgcagac ggtggagaag aacgcacagg
    11281 ctcatgaacg catggcccgc gaggtggaga aaacccgcca gcgcatggag gcactgagcc
    11341 agaaaatgcg cgaggaacag gcgcaggcca tggctctggc ggaggttcag gataaagcgg
    11401 ctgctgcgtt ttatcgtcag attgacagtg tgaaacaggc cagtgcgggg ctgcaggaat
    11461 tacagcgtat tcagcagcag atccgacagg ccagaaacag tggcgggatt ggtcagcagg
    11521 attatctggc gctgatttct gaggttactg cgaaaacccg tgttcttaca caggctgagg
    11581 aagaggctac ccgacagaaa gtggcgttta tccgtcagct taaagagcag gcaacccgcc
    11641 tgaatctttc atcttctgag ttgcttcgtg ctaaggcagc ccagctgggg gtaagcagtg
    11701 ctgcagaagt gtatatccgc aaaatggagc aggcaggaaa agccacgcat tcgctgggtc
    11761 tgaaaagtgc atcagcccgc caggagatag gcgttctgat aggtgaactg gcccgcggca
    11821 atttaggggc gctgagggga tccgggataa cgctggctaa ccgtgccgga tggatagaca
    11881 cactgatgtc accgaaaggc atgatgcttg gcggggttat tggcggtatt gccgcggccg
    11941 tctatggtct gggtaaagcc tggtatgacg gtcagaagga gggggaagaa tttaaccgcc
    12001 agttgtcgct gacggggcat tatgccggag tcactgccgg gcagctgtgg acgctcagtc
    12061 gtgctatttc cgggaatggt atcacgcaac atgctgcagc cggtgcgctg gctcaggtgg
    12121 tggggagtgg tgcatttcgt ggaaacgata tcggtatggt ggcgagagct gccgcacaga
    12181 tggagcgatc ggttggccag tcggtcagcg ataccataaa tcagtttaag cggctgaagg
    12241 atgatcctgt aaatgccgcg aaggctctgg acaatgagct gcattttctt actgccactc
    12301 agcttgagca gatacgcgtc cttggggagc aggggcggtc cagtgatgct gcacggatag
    12361 ccatgtctgc actggcagag gaaaccggtc ggcgtactgc ggatattgat aataacctca
    12421 atgcgcttgg cagtacgctg aagtatctgt ctgatttgtg gagtcgtttc tgggatgcgg
    12481 ccatgaatat tggtcgtgaa gactcgctgg atgaacagat tgccgcttta caggagaaag
    12541 tgtcgcgggc gaaaagactc ccctggacgg catcatcttc tcaggttgaa tacgatcagc
    12601 agcgtcttaa cgatcttcag gagaaaaaac gccagaagga tttgcaggat gcaaaagagc
    12661 aggcagagcg gaattatcag gagcaacaga aacgccgtaa tgctgaaaat gctgcactga
    12721 accggatgaa tgaaacggaa gcagcacgac atcagcgtga aattgcgcgt attaatgcca
    12781 tgcagtacgc cgatcaggct gtcagggatg cggcgataca acgtgaaaat gaacgttacg
    12841 agaaagccct ggcatccggt aagaaaaaaa cacgcgaaac ccgtaatgat gaggccaccc
    12901 ggttattgct gcagtacagt cagcaacagg cacaggtgga aggacagatt gctgctgcca
    12961 gacagtcagc aggcattgcc acggaaagga tgacagaagc gcataaacag cttctggctc
    13021 tgcagcagcg catcagcgac ctggacggga aaaaacttac ggcagatgaa aagagtgtgc
    13081 tggcccgtaa agatgaactg attcaggcac tgacgctgct ggatgtaaaa cagcaggagc
    13141 ttcagaaaca gacggcactc aacgagctga agaaaaaaac aattcagctg accagtcaac
    13201 tggctgaaga agagcgcgct cagcgtcagc aacatgacct ggatatcgcc acggtgggta
    13261 tgggtgatca gcagcgacag cgatatcagg tacaactgag tcttcgccag aaataccagc
    13321 aacagctgga gcagttgagg cgggatagtg agcagaaagg gacatataac acggatgact
    13381 acagaaaggc cgagcaggcg ctgacggaga gcctgaaccg acaactgaat gagaatcgcc
    13441 gttactggca acagcttgaa gttgtgcagg gtaactggaa aaacggagtc ctgcgtgcat
    13501 ttcaggattt taccgtggat gcagataata cggcagaaac agcagaacag gtgttctcgt
    13561 cagccttcag caacatggga aatggcctgg caacttttgt cactaccggc aaactcaatt
    13621 tcaaatcctt cacctcttct gtgctgtcag atatggcgaa aatcctggcg caggcaacca
    13681 tgatgaaatc gataaaaggg attggcagtg tactgggatt tgatctcagc agcctttccc
    13741 tgaatgccaa tggggggatt tatcagtctg ctgatttgag tcgttacagt ggcacggtgg
    13801 ttaaccgtcc gacgtttttt gcttttgcaa aaggcgcggg tgtgatgggg gaagcgggac
    13861 ctgaagccat tctgccattg cgtcgtggtg ctgacggtaa gctgggggtt gtggcggata
    13921 ttgggggttc aggtatggcg atgttttccc cgcagtacaa catcgagatc aataacgatg
    13981 gcacgaacgg gcagataggt ccggctgccc tgaaggcggt ttatgacctc gggaaaaaag
    14041 cggcagcgga ctttatgcaa cagcaggccc gtgatggtgg tcggttaagt ggagcatatc
    14101 ggtaatggag acgtttcact ggaaagtgcg cccggatatg aatgtggtat cagagccgaa
    14161 agtggtgaca gtgaagctgg gcgatggtta tgaacagcgt cgtgcggcgg gactgaataa
    14221 ccagttgtcg acttacagcg tgacgatacg tgttcgtaaa tgtgaacacc catctttaaa
    14281 agcctttctg gaacggcacg gtggcgtccg tgcatttcag tgga
//
LOCUS       DAZFQD010000139        13762 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.139, whole genome shotgun sequence.
ACCESSION   DAZFQD010000139 DAZFQD010000000
VERSION     DAZFQD010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13762
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.139"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(543..683)
                     /locus_tag="ACHKTW_004148"
     CDS             complement(543..683)
                     /locus_tag="ACHKTW_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO6465068.1"
                     /translation="MPQKTIIVSILCLTMLLTVWMIHRSPCEFRLNIMWSEFAAFLQC
                     KP"
     gene            complement(985..1263)
                     /gene="rpmE"
                     /locus_tag="ACHKTW_004149"
     CDS             complement(985..1263)
                     /gene="rpmE"
                     /locus_tag="ACHKTW_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HFO6465069.1"
                     /translation="MISISNEITSSSTCVDEYFRIGSTIKTDRVIELDGVTYPYVTID
                     VSSKSHPFYTGKLRTVASEGNVARFTQRFGRFGRFGRFGRFVDAKKGG"
     gene            1414..1698
                     /locus_tag="ACHKTW_004150"
     CDS             1414..1698
                     /locus_tag="ACHKTW_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6465070.1"
                     /translation="MKNEAEKAMWSCKTSDTPWTFSSLLKKAVCAILPDAEPKIENAT
                     KGCSVLSTVDNAPGNKSLNYDPFSFESGLHLDIENLPLIENWDGEPYKLP"
     gene            complement(1744..3246)
                     /locus_tag="ACHKTW_004151"
     CDS             complement(1744..3246)
                     /locus_tag="ACHKTW_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraG N-terminal
                     domain-containing protein"
                     /protein_id="HFO6465071.1"
                     /translation="MTTNNYLEYFLTLLGWLVNNGLWDLLIGTGLFALPLAFKVIGIW
                     LKVREEGADEGNKGMLSLPRIENALYGAFFVMVASCVPLVQVTLDTLKFDRSRAQTCG
                     VWTPKAPGDTGYQGIISSLGDKTAAAPIWWVVVHKLSKGVTQAAVASIPCRPDLRQLR
                     FEVQRTFIANRALADELQDFTNDCYSLAMYQWKQRDQGMTKDRKVLSDISWIGSSTFM
                     QGDYGTLQSRMPRAAFPWNDARDSGRPDTGRGGYPTCREWWNDSSVGLKKRVTEEVDE
                     GLWLRLSAAMKLLGNSQATYQEEIIKRLVSPANLTISQGGEVYRGYGGNADMTTMNAI
                     SRIGATAGSGVASLGAFPAFDAMRQALPMVQSILLMAVYIMLPMILAFAAYEFSTLIT
                     LTFVVFALNFLTFWWELARWLDSWMITALYGSDTHSSLNMMGLQNTSDDIIMNLVMGT
                     MFIVLPAVWLGALSWAGVSIGQVINAAIGKGVSTSQQAGQHAGGITNKLK"
     gene            complement(3250..3615)
                     /locus_tag="ACHKTW_004152"
     CDS             complement(3250..3615)
                     /locus_tag="ACHKTW_004152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001044690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6465072.1"
                     /translation="MNKTVLMVPGSRAGLLVKGMKLLGKWGLTVIVIMASALLIADTG
                     MRHAQEVDALRLWFHETRFGWLGWRFCIYCGLVWGCRKIWRAPGFRAEYRLRFARICM
                     VSALFFLLCEYAIFSAAGD"
     gene            complement(3608..5029)
                     /locus_tag="ACHKTW_004153"
     CDS             complement(3608..5029)
                     /locus_tag="ACHKTW_004153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrating conjugative element protein"
                     /protein_id="HFO6465073.1"
                     /translation="MTMKVKTLVMMVMLSCSTPVMADSILSLPKLNDSTFGYGKSVDG
                     AVSDKLFYTLGGGSVISQPATRSNMQKLGLDLGWSSDLMCGNFDLKTTVGNQLNGITD
                     GFKNLMGDVIQGATGAVSSLPAMVIQRANPGLYDMITNGVLQANVAFDKAQLNCQNMA
                     KRMMDFSDRSKWTQAAMMEEYKKVVNTSDGDAVKGDDAGRQATGKEGQKWIGGQQRGG
                     AGQPAIRPVHDMTAAGFNMMNSLPVTSTSGVGAGSCNGSACEKFRNAEEAASAVVKVL
                     GDRSMRTCTDAKECTSGDSDQQPGTAVAGTGFAPMLEEATRINTEQLVRLVNGQDKPT
                     AENLAKLKTGSLAVSAGVIHALRRDPDNMSLTSRLAGELAMADTVETALVMRRMLLTG
                     MSEPYAAAQPAALEEGDRRIASLDREIIALKSEMELKRDLARNSVLTIIERDNERVSN
                     NPMIQQTDNADSRVRSLEVPENE"
     gene            complement(5041..6015)
                     /locus_tag="ACHKTW_004154"
     CDS             complement(5041..6015)
                     /locus_tag="ACHKTW_004154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000987808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03756 family integrating conjugative element
                     protein"
                     /protein_id="HFO6465074.1"
                     /translation="MMSRSFRLRAGALLLSVAALPLASASLNTASIVASAAAPDCISW
                     RVSGICYWLYCSGFSCKVRTSVKVTHYIPEVVVSTYTAPGGNPWKEMSLVSQTAGGLE
                     NAITKGLAGLSAGGGNPADMKAQGERRSAIRFKYADALGHPATRLIGGMVPGFSCDSP
                     VTPAMPYFLSTLDSLVWRTGMPESLYPEALVPGKREIGSQAEENLWGSVYPRSGFVMQ
                     TDDDKAAAVVAQRVADIISRTGEPHVYKSIQTPKKDGFWPPDPVEENTGTRNHKWQRL
                     TPSVSRSCAVFPDGDHQAAEDGNAAFALWQPYSCCKKRGQRFLGSTNF"
     gene            complement(6012..6410)
                     /locus_tag="ACHKTW_004155"
     CDS             complement(6012..6410)
                     /locus_tag="ACHKTW_004155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000286193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03757 family integrating conjugative element
                     protein"
                     /protein_id="HFO6465075.1"
                     /translation="MHRIIFCSLFFLPMAVSAGITLYTDSQHLPVNPPADVRVILLDG
                     PEQLQARFFGALPADAAEAEARVRARMQSPEWGAMQAELAERYREVAYAWSLGVKKYP
                     AVVFDDREVVYGTTDVAEAAQLRAQEGVQP"
     gene            complement(6675..7061)
                     /locus_tag="ACHKTW_004156"
     CDS             complement(6675..7061)
                     /locus_tag="ACHKTW_004156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFO6465076.1"
                     /translation="MMYLKYPEPELQTAVHPGGVVFFLPPQGKPGVTEITQPHLLRLR
                     SHLNRRLGRLRRVVCQPQRVGMNSSVAVTLEGDTGKVYLLVSVCGHECWPSEEEYTHL
                     RWYIQVVDAADLFYLLLWLNGMEAEK"
     gene            complement(7058..9754)
                     /locus_tag="ACHKTW_004157"
     CDS             complement(7058..9754)
                     /locus_tag="ACHKTW_004157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer ATPase"
                     /protein_id="HFO6465077.1"
                     /translation="MTQEEEVRVYHAGPSVIDFLPWVEYLDEAQCLLLDDGMSVGAVY
                     DVTPAATEGRTEERLEQIRDMVEDALQDSFDEYDTHPWVVQFFCQDENDVDAYLDYLK
                     GYVKPHAQGTAFTEAWLGEMERHLRGIARPEGLFTDTLVTGQPWRGQQRRTRMVIYRR
                     IAKNNSDPMPPVAMLNQVCDRVVSALGSAGVRCHRMNGLQVHSWLLRLFNPAPSWISR
                     EDLYRMASRADPRENAADVMPVMTDFAESLWFTPPHSDPENGVWWIDDLPHCAVVVEK
                     LRTPPEPGAITGEMSRGEKTVNALMDTFPEGTVLCMTIVVQPQDTLEERFTRLSKNAV
                     GENTESIRARQDVKVVKELLGNRHKLYRAGITFLLRAGDMDSLKRKRVELTTVLLGAG
                     LQPVRPEFDVGPLNSWLRALPMCFDPDMDRKQWHTRLMWVQHLAGLLPVTGRETGTGH
                     PGFSFFNRGGDVLTFDPLNKLDRTQNAHLLLFGPTGAGKSATLCASLSQVMAVHRPRL
                     FIAEAGNSFGLLADYFESLGLSVNKISVKPGSGVTLPPFADAHKLVEEGQTLQSVDEH
                     SLPDLDGDEEEEKRDILGEMEISARMMITGGDPKEEASLKRADRAMIREALLMATHAT
                     YREGRQMLPEDLQVALREISCDAGRNEVRRAKAAEMAESLGMFTQKGSFEAELFNREG
                     ELWPEADVTLIDLGHLAREGYEAQMALTMVSMTNMINNIAERDQYLGRDIVFAVDEAH
                     IVTVNPLLSPYMTKVVKMWRKLGAWLWLATQNLKDYPDISEKMLNMAEWWVCLTMPPE
                     EVNNIARFKSLTEEQKAVLMSASKLSGCYTEGVVLAKKIEALFRAVPPSLYLALGMTE
                     KDEKAERRMLMKEHNCSELEAAFHVARKLDRLRGLSAVESAA"
     gene            complement(9874..10266)
                     /locus_tag="ACHKTW_004158"
     CDS             complement(9874..10266)
                     /locus_tag="ACHKTW_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03751 family conjugal transfer lipoprotein"
                     /protein_id="HFO6465078.1"
                     /translation="MCIPLTALLLSGCSTSKEELLPAGDQTMLELWQGKTTGGSQRHA
                     LAARETLWRPLTSDEKQQVHREGQSYSRTQESEISQQFPRLPNPDMVMYVFPHLAGGT
                     SPVPGYSTVFPFYSRTQYALPGERTEAM"
     gene            complement(10305..11783)
                     /locus_tag="ACHKTW_004159"
     CDS             complement(10305..11783)
                     /locus_tag="ACHKTW_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001506261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03752 family integrating conjugative element
                     protein"
                     /protein_id="HFO6465079.1"
                     /translation="MQIKSNALVKFVVPAVVLVGLAVTMKSCKTQENVADRGEQARAL
                     SGLSSEELRALGVEGDTPEDTLRTLVARMNQVRDNQRTLEKQNSQLLKDNEQLRRRST
                     DVSGQVNDAVAGVQKTYEQKQRELQEQQHSLMSQIDAMKDQLSRAETLSRRQESDIPP
                     GLGLDAGGGSGVVISQGADGLMWVSPADQSQDDPLSSSGSKGGPRFPTSFLEDNALSR
                     QKAEYEQQVKGYSNEQQRATTQAVYTLPENSTLTGSRAMTALLGRVPINGTVTDPYPF
                     KILVGRDNLTANGIELPDVEGAIVSGTASGDWTLSCVRGQVNSITFVFADGTVRTLPS
                     PDVNGTTNSNQQAKGNGTGGIGWISDEDGIPCISGTRKSNASSYLPTIFALSAAGAAG
                     DALSEGQYTSQNNVNGITSTLTGSAGQAVLGKALSGGMNETADWVRQRYGQTFDAIYV
                     PPGKALAVHITRQLAIDYEEQGRRVRYDFALPGEANDHGGLD"
     gene            complement(11773..12681)
                     /locus_tag="ACHKTW_004160"
     CDS             complement(11773..12681)
                     /locus_tag="ACHKTW_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03749 family integrating conjugative element
                     protein"
                     /protein_id="HFO6465080.1"
                     /translation="MKAGIVCSLLRPAVMMMSLSVLSLTAQADELMKWERIPLNIPLK
                     VGQERVIFADKNVRVGFPPALNGKLRVQSTGGAVYLKADSAFPQTRVQLQDVESGEMI
                     LLDVTAGEKGTTEPVRIVYDGDVSTVSRAAGSDVQTVKSRSSNGKPGTDNSTQARRTE
                     PRYAEPLPVLLTRYAAQSLYAPLRTLEPVAGIRPVVPHLPRQITTVYPSEPLTVSPVA
                     AWAAGGRTVVALKLTSTVSRRIVLDPRALQGRFVAATFQHRWLGAAGTPEDTTMLYVV
                     TAGSPEKAFVEEPVTSRRGGEVTHAD"
     gene            complement(12678..13325)
                     /locus_tag="ACHKTW_004161"
     CDS             complement(12678..13325)
                     /locus_tag="ACHKTW_004161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PFL_4703 family integrating conjugative element
                     protein"
                     /protein_id="HFO6465081.1"
                     /translation="MSRFRHALKDRDQHILTLRVFSGVLCGLLLMVTIGWMTAPDRLT
                     IHNPPDLRSGSTRPWWEVPPSTVYSFAFYIFQQLNAWPKNGEVDYPAKIHALSAYLTP
                     SCREILETDARRRKDAGELLDRVRVVYEIPEHGYGGRYNRDPVTVEGRDNWLVRLDLV
                     ADEYFHAEPVKRALIRYPMKVMRMEGDPERNPFGLVLDCYSSTPQRLEAVKGGGE"
     gene            complement(13322..13687)
                     /locus_tag="ACHKTW_004162"
     CDS             complement(13322..13687)
                     /locus_tag="ACHKTW_004162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03750 family conjugal transfer protein"
                     /protein_id="HFO6465082.1"
                     /translation="METIRFLPDRLNAEPTVFRGFTTPELGLAALTGFFTGLLWSLPF
                     VPVFGWVIIPTGALLTPLVLVWTGGAWISRLKRGKPDNWLWQRLEEKKAALGVGRSGL
                     IREARGWSLRRTRVRGGKS"
BASE COUNT         3073 a         4424 c         3254 g         3011 t
ORIGIN      
        1 caatgatcgt tttattcttt cacttttatc aaaacatcac cagaaacagg aaatgactgt
       61 cgcaataatg aacataaaaa gacaaaccag gatggcagta aatcaataaa gtacgatttc
      121 ataagaatca tcagaaaaac ggtggacgcg atatccggca cgaggcaaaa cacagcaaca
      181 aaaaataaac cggatatcgc gcccggtact tttctaactc aacttcaggc attgtgcaag
      241 ttttgatcgt cggtggattc acagtcatgc cccattgatt caggctgaag actcatatat
      301 cattggaaga gactagaatc tcattgctgt gaaattcctt acagaatctt cagccccatt
      361 ttatggggct ttttttggtc tgaacccagg cagttaccac accttcggta tgtgaataaa
      421 aaagcccgca gtttttacgc tgcgggcttt gttttttctt tgtcacttgc atgcacgggt
      481 gcggctttcg accgaacctt cggcaaccgg atagcggggg ctttcacccc cgctgcggtt
      541 tcttacggtt tacactgcaa gaacgccgca aattctgacc acatgatgtt aagtctgaat
      601 tcacatggtg aacgatgtat catccagacg gtcaacaaca tggttaagca taaaatgctc
      661 acgatgatcg ttttttgcgg catagcgctt gctccttttt caaagaggcg ctaaccttcc
      721 acttgctaaa ggtatgtaga ttagggcctc ggctaaacag agatgttttc cggggcattt
      781 ccacatccgg ccttcaggta atccttccag ccgtcagccg aaaggcaccc gcgcgtaatg
      841 tactggtttt cagagaggtc cgcaatcttc tggtgacggg atcatctgca cgccccggag
      901 ggacgggcta cctcagtata ttcaggtgca ggctctctct gtccggtccg ttttaaaatt
      961 gtcaaacgca cctttgcggc gaaatcagcc gccttttttc gcatcaacaa aacgcccaaa
     1021 acgcccaaaa cgcccaaaac gcccaaaacg ctgggtaaag cgtgcaacat taccttctga
     1081 agcaacagtt ctcagtttgc ctgtatagaa cggatgcgac ttagaagaaa cgtcaattgt
     1141 aacatatggg taagttaccc catccagttc aattacccgg tcagttttga tcgtcgaccc
     1201 gatcctgaag tactcatcaa cacacgtgct tgatgaagtg atttcgttac tgattgatat
     1261 catcgatcca caataaaaat aagctaaagt agctggcatt gacacgattt acgtttctta
     1321 tcatctctct gccgcttctt tcttccattg aacatttata atacagcacg tatgatatca
     1381 ctgtgataac tacctgctca ggggagctgc gctatgaaaa acgaagcaga aaaggcgatg
     1441 tggagttgca aaacctcgga taccccatgg accttcagtt ccctgctgaa aaaggctgtc
     1501 tgcgcgattt tgcctgatgc agaaccgaag attgagaatg ccacaaaagg atgttctgtt
     1561 ttatcgactg tagataatgc ccctggcaat aaatcattaa attacgaccc tttttctttt
     1621 gaatctggtt tacatcttga tatagaaaat cttcccctta tagaaaattg ggatggagaa
     1681 ccttacaagc tcccctaaaa atatggcgat atctatcaca aatatcgcca taaatattaa
     1741 caattatttt aatttattcg taatacctcc agcatgttga ccagcttgtt ggcttgttga
     1801 cactcctttt ccgattgccg cattaatcac ctgtccgata cttaccccag cccaggataa
     1861 tgcccccagc cacacagctg gcagtacaat aaacatcgtc cccatcacca ggttcatgat
     1921 gatatcgtcc gacgtgttct gcagccccat catattcaga ctcgaatgtg tatctgaacc
     1981 atacagcgcc gttatcatcc agctgtccag ccaccgcgcc agttcccacc agaatgtcag
     2041 aaaattcagt gcaaatacca caaacgtcag cgtgatcagc gtactgaatt catatgccgc
     2101 aaaagcaagt atcatcggga gcatgatata caccgccatc agcagaatac tctgcaccat
     2161 cggcagcgcc tgccgcatgg catcaaacgc cggaaacgcc cccagactgg cgacaccgga
     2221 acctgccgtt gcgccaatgc gtgaaatggc attcatcgtg gtcatgtctg cattaccacc
     2281 atatccccga tacacttctc ccccctgaga aatcgtcagg ttagccggac tgaccagccg
     2341 tttgataatc tcctcctgat aagtcgcctg gctgttcccc agaagcttca tcgctgccga
     2401 aagacgcagc cacagccctt catcaacttc ctccgtgacc cgctttttca gccccacact
     2461 gctgtcgttc caccactccc ggcacgttgg gtagcctccg cgccccgtat ccggacgacc
     2521 gctgtcacgg gcgtcattcc acggaaacgc cgcccttggc attctggact gcagcgtgcc
     2581 gtaatccccc tgcatgaacg tgctgctccc aatccagctg atatccgaca gcacctttct
     2641 gtccttcgtc ataccctggt cacgctgttt ccactggtac atcgccagcg agtagcagtc
     2701 attggtgaaa tcctgcaact catcggccag cgccctgttc gcaatgaacg tcctctgcac
     2761 ttcaaaacgc agttgccgca gatcaggcct gcagggaatg gacgccacgg ccgcctgtgt
     2821 cacccctttg gaaagcttgt gcacaacaac ccaccagatc ggggccgccg ccgtcttatc
     2881 tcccaggctg ctgataatcc cctgataacc ggtgtccccg ggggctttcg gcgtccacac
     2941 accacaggtc tgggcccggc tgcgatcaaa cttcagggta tccagcgtca cctgaaccag
     3001 cggtacacag gaagccacca tcacaaaaaa cgcaccatac aatgcattct cgatgcgggg
     3061 taacgagagc attcccttgt tgccttcgtc cgccccctct tccctcacct tcagccagat
     3121 accgatcact ttgaaagcca gaggaagcgc aaacagtccc gtgccaatca gcaaatccca
     3181 cagaccgtta ttgaccagcc agcccagcag ggtcagaaaa tattccagat agttattggt
     3241 cgtcactgct cagtcccccg cggcggaaaa aatcgcgtat tcgcacagca ggaaaaacag
     3301 cgcactgacc atacaaatcc gcgcaaaacg cagacggtac tctgcccgga atcccggtgc
     3361 acgccagatt ttccggcatc cccataccag cccgcagtaa atacaaaacc gccagcccag
     3421 ccatccgaaa cgggtttcgt ggaaccagag cctcagcgca tccacctcct gcgcatgacg
     3481 catacctgta tccgcaatca ggagcgctga cgccatgatg acaatcactg tcagccccca
     3541 tttccccagc agtttcatcc ctttcacaag cagcccggcc cggctcccgg gcaccatcag
     3601 cacggtttta ttcattttca ggaacctcca gtgaacggac acggctgtca gcgttgtcgg
     3661 tctgctgaat catcgggtta ttactcacgc gttcattgtc acgctcaatg atggtcagca
     3721 cactgttacg cgccagatcc cgtttcagct ccatctcact tttcagggcg atgatctccc
     3781 tgtccagaga ggcaatacga cgatcgcctt cttccagcgc agcaggctgt gcagccgcat
     3841 acggttcaga cattcctgtg agcagcattc tgcgcatcac cagtgccgtt tccaccgtat
     3901 cagccatcgc cagttcaccg gccagacggg aggtcaggga catattgtcc ggatcgcgac
     3961 gcagcgcatg tatcaccccg gcagataccg ccagactgcc ggtcttcagt tttgccagat
     4021 tctctgccgt gggtttatcc tgcccgttca ccaggcgaac cagctgttcc gtatttatgc
     4081 gtgtcgcctc ctcaagcatc ggcgcaaacc ctgttccggc taccgccgtt cccggctgct
     4141 ggtctgagtc accactggta cattccttcg catccgtaca ggtgcgcatt gaacgatcac
     4201 ccagtacctt caccacagcg ctggccgcct cctctgcatt cctgaatttc tcacaggcac
     4261 tgccattaca gcttcccgca ccaacaccgg atgtactggt caccggcaga ctgttcatca
     4321 tgttaaaccc cgcggccgtc atgtcatgaa ccggacggat ggcgggctga ccggcaccac
     4381 cacgctgctg accaccaatc catttctgcc cctctttccc cgttgcctgg cgtccggcgt
     4441 catcaccttt aacggcatca ccgtcactgg tattaacaac ctttttgtac tcctccatca
     4501 tcgcggcctg cgtccacttg ctgcgatcgg aaaaatccat catccgtttc gccatgttct
     4561 ggcagttcag ttgtgccttg tcgaacgcca cgtttgcctg taacacacca ttcgtgatca
     4621 tgtcgtagag gccgggattc gcacgctgta tcaccattgc aggaaggctg gagaccgcac
     4681 cggtcgcccc ctgaatcaca tcccccatca ggtttttaaa gccgtcagtg atcccgttga
     4741 gctggtttcc tactgtggtt ttcagatcga agttcccgca catcagatcg ctgctccagc
     4801 ccaggtccag cccgagcttc tgcatgttac tgcgcgtcgc cggctgggaa ataacggagc
     4861 caccgcccag ggtgtaaaac agcttgtccg acaccgcacc atccaccgat tttccgtaac
     4921 caaacgtgct gtcattcagt tttggcagcg agagaatgct gtctgccatg accggcgtac
     4981 tgcaggacag catcaccatc atcaccagag tctttacctt catcgtcatc atccgtctcc
     5041 ttaaaaattc gtactgccaa ggaaccgctg cccccgcttc ttgcagcaac tgtagggctg
     5101 ccagagcgca aaagcggcat taccatcctc cgcggcctga tggtctccgt caggaaacac
     5161 ggcacaggag cgggagacgg agggcgtcag tctctgccac ttgtggttac gggtgcccgt
     5221 attttcctca accggatccg gcggccagaa accatccttt ttcggggtct gtattgattt
     5281 ataaacatgc ggctcaccgg tccggctgat gatgtcagcc acccgctggg caaccacggc
     5341 cgctgccttg tcatcatcgg tctgcatcac aaaacctgaa cggggataaa cactgcccca
     5401 caggttttct tcagcctgac tgccgatttc acgtttaccg ggaacgaggg cttccggata
     5461 cagggattca ggcattccgg tccgccagac gagactgtcc agggtactca ggaaataagg
     5521 cattgccggt gtcaccggcg aatcgcagct gaatcccggc accattccgc caataagacg
     5581 cgttgccgga tgccccagcg catcggcata tttaaaacga atggccgaac gacgctctcc
     5641 ctgagctttc atgtccgcag gatttccccc tccggcactc agccccgcca ggcctttcgt
     5701 aatggcattc tccagccctc cggctgtctg actgaccagc gacatctctt tccagggatt
     5761 tccccccggt gccgtgtagg tggaaaccac cacttccgga atataatgtg tcactttcac
     5821 cgatgttctg accttacagc tgaacccgga gcagtacagc cagtagcaga ttccactgac
     5881 ccgccagctg atacaatccg gcgctgccgc actggccaca atactggcgg tattcagtga
     5941 agctgacgcc agaggcaggg ctgcaacact cagcagcagg gccccggcac gcagacgaaa
     6001 tgaccggctc atcatggctg tactccttcc tgtgctctca gttgtgcggc ctctgccaca
     6061 tccgtggtac cgtagaccac ctcacgatca tcaaagacca cggccggata tttcttcacg
     6121 cccagtgacc atgcatacgc cacctcccgg taacgctcag ccagctccgc ctgcatcgcc
     6181 ccccactccg gggactgcat acgcgccctg acccttgctt ctgcctcagc ggcatccgcg
     6241 ggcagtgcac caaaaaaacg cgcctgcagt tgttcagggc catccagcag gatcacccgt
     6301 acgtccgcag gcggattgac cggaaggtgt tgagaatcgg tataaagggt tatgccggct
     6361 gacaccgcca tcggcaggaa aaacagggaa cagaatataa tccggtgcat ggtacgcctc
     6421 cgtcagacaa tatctgaatg acagcgtgcg cagaaaactt cacgatagca gcaataaatt
     6481 caaaaccgga tgatgaaaat taacgcagag aatggtcagt ggaagggcat gagtggaatg
     6541 atgccgtcga tgaattcttc cggtgaagac ggataaaaat attgccgtaa tgtaataaac
     6601 gtgttaatta aaaacgcgtg taccggcagg tatgaattga tcccgtaacc catctgccgg
     6661 ttacacgctg gcgcttattt ttctgcctcc ataccattca gccacaacag cagatagaac
     6721 aaatcggccg catcaacaac ctggatatac catctcagat gcgtatactc ctcctctgac
     6781 ggccagcatt catgaccaca cacggaaaca agcagataga cctttccggt atcaccttcc
     6841 agcgtcaccg ccacgctgct gttcatgcca acccgctggg gctggcatac aacacgacgc
     6901 agcctgccca gacgacgatt aaggtgtgag cgcagccgca gaagatgcgg ctgtgttatc
     6961 tcggtcaccc cgggcttacc ctgtggcggc aaaaaaaaca caacgccacc cggatgcacc
     7021 gctgtctgca gctccggctc cggatatttc agatacatca tgccgcactc tccacggcgg
     7081 aaagtccccg cagtctgtcc agtttacggg ccacatggaa cgcagcctcc agttcgctgc
     7141 agttatgttc cttcatcagc atgcgccgct cagccttctc gtccttctcc gtcatcccca
     7201 gcgccagata aagactcggt ggcaccgcac ggaacagcgc ctcaatcttc ttcgccagga
     7261 caaccccctc cgtataacag ccggacagtt tgctcgccga catcagcacc gccttctgct
     7321 cctccgtcag ggatttaaaa cgggcaatgt tgttcacctc ttccggcggc atcgtcagac
     7381 acacccacca ttccgccatg ttcagcattt tctctgatat atccggatag tccttcaggt
     7441 tctgcgtcgc aagccacagc caggctccca gcttacgcca cattttcacc accttcgtca
     7501 tatagggcga gagcagcgga ttcaccgtca cgatatgcgc ctcatccacg gcaaacacaa
     7561 tatcacgccc cagatactgg tcacgttcgg caatgttatt gatcatgttg gtcatcgaca
     7621 ccatggtcag cgccatctgc gcctcatacc cctcacgggc caggtgcccc aggtctatca
     7681 gcgtcacatc cgcttccggc cacagttcgc cttcgcggtt gaacagctcc gcctcaaaac
     7741 tccctttctg ggtgaacatt cccagtgatt ccgccatctc tgccgccttt gcccggcgta
     7801 cctcgttacg tcccgcatca caggagattt cccggagtgc cacctgcaaa tcttccggca
     7861 gcatctgccg cccttcccgg tacgtcgcgt gtgtggccat cagcagcgct tcacggatca
     7921 tcgcccggtc cgcacgtttg agtgaggcct cttccttcgg atcaccgccg gtgatcatca
     7981 tcctggcaga gatttccatt tctcccagaa tgtcgcgttt ctcctcctct tcatcgccat
     8041 ccagatcggg cagcgagtgc tcatccacac tctgcagggt ctgcccttct tcaaccagct
     8101 tatgcgcatc cgcaaagggc ggcagtgtca caccgctccc cggtttgaca ctgattttgt
     8161 tcactgaaag cccgagactt tcaaagtaat cggccagcag accaaacgag ttacccgcct
     8221 cggcaataaa caaccgggga cgatgcacgg ccatcacctg tgacagagag gcacacagcg
     8281 tggcagactt ccctgccccc gtgggcccga acagcaacag atgggcattc tgggtacgat
     8341 ccagtttgtt caggggatca aacgtcagga catctccccc gcggttaaaa aaactgaagc
     8401 cgggatgccc tgtaccggtt tcccggccgg tcaccggcag aagccctgcg agatgctgta
     8461 cccacatcag acgtgtgtgc cactgcttcc tgtccatatc cggatcaaag cacattggca
     8521 gcgcacgcag ccagctgttc agcggcccca catcaaactc cggtcgcacc ggctgcaggc
     8581 ctgcacccag cagaaccgtg gtcagctcca cccgtttacg tttcaggctg tccatatcac
     8641 cggcccgcag aaggaaagtg atccccgcac gatagagttt atgccggtta cccagcaact
     8701 ccttcaccac cttcacatcc tgacgtgccc ggatggactc cgtattctca cccacggcat
     8761 ttttcgacag ccgggtaaac cgctcctcca gggtgtcctg tggctgtacc acaatggtca
     8821 tgcacagcac cgttccttcc ggaaacgtat ccatcagcgc attcaccgtt ttttctcccc
     8881 gggacatctc accggtaatg gcgcccggct ccggtggcgt acggagcttc tccaccacca
     8941 cggcacaatg tggcagatca tcaatccacc acaccccgtt ctccggatcg gaatgaggcg
     9001 gggtaaacca caggctttct gcaaagtccg tcatcaccgg cataacatcc gcagcgttct
     9061 cacggggatc ggctcttgag gccatccggt acaaatcctc ccggctaatc caggacggtg
     9121 ccggattaaa taaccggagt aaccagctgt gaacctgcag accgttcatc cggtgacagc
     9181 gcaccccggc gctccccagt gccgacacaa cgcgatcgca aacctgattc agcatggcca
     9241 ccggcggcat cggatcactg ttgttcttcg cgatacgacg ataaatcacc atacgggttc
     9301 gccgctgctg tccccgccac ggctgaccgg taaccagcgt gtctgtaaaa aggccttccg
     9361 ggcgggcaat tccccgcaaa tggcgttcca tttcgcccag ccaggcttcc gtaaacgccg
     9421 ttccctgcgc gtgaggtttc acataccctt tcagataatc aagatacgca tccacgtcat
     9481 tttcatcctg gcagaagaac tgcaccaccc agggatgcgt gtcatactca tcaaagctgt
     9541 cctgcagtgc gtcctccacc atgtccctga tctgttcaag acgttcctcc gtacgccctt
     9601 cggtcgccgc cggcgtcacg tcataaacag cccctaccga cattccgtca tccagcagca
     9661 gacactgtgc ctcatccagg tactccaccc agggaagaaa atcgataact gacggcccgg
     9721 catgataaac ccggacctcc tcttcctgcg tcatctgccc gggacgtttc aggggctgcg
     9781 ccccggccgg gacaggcggt acaaaaccct gtcctgccgt ctccgtttct gatggcgtgg
     9841 tgacattgcg acgtttaaac agtgtgaatg ccattacatc gcctccgtac gctcaccggg
     9901 taacgcatac tgcgttctgc tgtaaaaagg aaataccgtg ctgtatcccg gcaccggtga
     9961 cgtcccgccg gcaagatgcg ggaatacgta catcaccatg tcagggtttg gcagacgggg
    10021 aaactgctgg ctgatttcac tttcctgcgt cctgctgtag ctctgcccct ccctgtgtac
    10081 ctgctgtttc tcatctgacg tcaggggccg ccacagtgtc tccctggcgg ccagcgcatg
    10141 tcgctggctc ccccccgttg ttttcccctg ccagagttca agcatggtct gatcgcctgc
    10201 cggcagaagc tcctctttcg aggtggagca cccggataac agcagggcag tcagggggat
    10261 acacatcact aaccgtaatc gtttcacaaa cgtattctcc ctgttcagtc aagccccccg
    10321 tggtcgtttg cttcacccgg cagggcgaag tcataacgca cacggcggcc ttgctcttca
    10381 taatcaatag ccagttgtcg ggtaatatgt accgccagcg ctttccccgg gggaacataa
    10441 atggcatcaa acgtctgccc gtaacgctgc ctcacccagt ctgccgtctc attcattcca
    10501 ccggacagcg ccttaccaag tacagcctgc cccgcgctgc cggtcagcgt ggaggtgata
    10561 ccgttgacat tgttctgcga ggtatactgc ccctcactca gggcatcccc ggctgcaccg
    10621 gcggctgaca gtgcaaatat cgttggcaga taactcgacg cattggattt acgggtaccg
    10681 ctgatgcagg gaataccgtc ttcatcggag atccagccaa tcccgccagt accattcccc
    10741 ttcgcctgct gattgctgtt ggtggtgccg ttgacatccg gtgacggcag cgtgcgcacc
    10801 gtgccatcag caaaaacaaa ggtgatactg ttcacctgcc cgcgcacaca ggaaagcgtc
    10861 cagtcaccac tggccgtgcc ggagacaatc gccccttcca catccggcag ctcaatgccg
    10921 tttgccgtga ggttgtcgcg cccgaccagg atcttgaacg ggtaaggatc cgtcacagtg
    10981 ccgttgatcg gcacccgccc cagcagggcc gtcatcgcac gactgcctgt cagcgttgaa
    11041 ttttccggca gcgtgtaaac cgcctgtgtg gtggctcgct gctgttcatt gctgtatccc
    11101 ttcacctgct gctcatactc agccttctga cgactcagtg cgttgtcctc aaggaatgag
    11161 gtcggaaaac gagggcctcc ctttgaacca ctgctgctca ggggatcgtc ctgagactga
    11221 tcggccggac ttacccacat cagcccgtca gccccctgcg aaatcaccac gccactgccg
    11281 ccgcccgcat caagcccgag ccccggcggg atatcgcttt cctggcggcg actgagcgtc
    11341 tctgcccggc tcagttgatc tttcatggcg tcaatctgcg acatcaggga atgctgctgc
    11401 tcctgcagtt cccgctgctt ctgctcgtag gtcttctgta cccctgccac agcgtcattc
    11461 acctgaccgg acacatccgt gctccgcctg cgcagctgct cgttatcctt aagaagttgc
    11521 gagttctgtt tctccagcgt tctctgattg tcccgcacct ggttcatacg ggcgaccagc
    11581 gtccgtaatg tgtcttccgg cgtgtcacct tccaccccaa gagcccgtag ctcttctgac
    11641 gataatccgg ataaggcccg ggcctgctcc ccgcggtcag caacattttc ctgcgttttg
    11701 cagcttttca tcgtgactgc aagccccacc agaacaacag cgggcaccac aaatttcacc
    11761 agcgcgttgg acttaatctg catgcgtcac ctccccgccc ctgcgggacg tcacaggctc
    11821 ttccacaaac gccttctccg gactgcccgc cgtcaccaca tacagcatcg tggtatcttc
    11881 cggcgtaccg gcagcaccca gccagcgatg ctggaaagtc gccgccacaa aacgcccctg
    11941 aagggcccgg ggatcaagca caatacgcct gcttaccgta ctggtgagtt tcagggccac
    12001 caccgtccgg ccaccggctg cccatgccgc caccggtgaa acagtcagcg gctctgacgg
    12061 ataaaccgtg gtgatctgcc ggggaagatg tggcaccacc ggtctgatcc ccgccaccgg
    12121 ttcaagggtg cgcagtggtg catacaggga ctgtgccgca taccgggtca acagcaccgg
    12181 caggggctca gcataacggg gctccgtgcg acgggcctga gtactattat ccgtccccgg
    12241 cttcccgttg ctgctgcgac ttttcacagt ctgtacatca ctcccggcag cacggctgac
    12301 cgtactcacg tctccgtcat acacgatacg cacgggctcc gtggttccct tttcccccgc
    12361 agtgacatcc agcaggatca tttccccgct ttccacatcc tgcagctgaa cccgggtctg
    12421 cggaaatgca ctgtctgcct tcaggtagac cgcaccaccg gtactctgga cccgcagttt
    12481 tccattcagc gccggaggga agcccacccg cacattttta tccgcaaaaa tcacccgctc
    12541 ctgccccacc ttcagcggga tattcagcgg tatacgctcc cacttcatca gttcatccgc
    12601 ctgtgccgtc agactcagca ctgacaacga catcatcatg acggcaggac gcagcagact
    12661 gcacacaatg ccggctttca ttcaccacct cccttcacgg cttccagacg ctgtggcgtg
    12721 gagctgtagc agtccagcac aagaccaaac ggattacgct ccggatcgcc ctccatccgc
    12781 atcactttca tcggatagcg gatcagtgcc cgtttgaccg gctctgcatg aaaatactca
    12841 tccgccacca ggtcgagacg gacaagccag ttgtcccgcc cctccaccgt gacagggtca
    12901 cggttgtaac gcccgccgta accgtgctca gggatctcat acaccacacg aacacgatcc
    12961 agcagttctc ctgcatcctt acgacgacgg gcatccgtct ccaggatctc ccggcatgac
    13021 ggtgtcagat aggccgacag tgcatgtatt ttcgccggat aatccacctc gccatttttc
    13081 ggccaggcat tcagttgctg aaaaatgtag aacgcaaagg aatagaccgt tgacggagga
    13141 acttcccacc acggacgcgt actgccggaa cgcagatccg gcggattatg aatggtcagc
    13201 cggtcagggg ccgtcatcca cccgatagtg accataagaa gaagtccgca cagcacaccg
    13261 ctgaacaccc tcagcgtcag aatatgctga tcacgatcct tcagggcatg acgaaaacgg
    13321 ctcatgattt accccctctg acacgggtac ggcgcaggga ccagccccgg gcctccctga
    13381 taagcccgga gcgtcccacg cccagcgccg cttttttctc ctcaagacgc tgccatagcc
    13441 agttatccgg tttcccccgc ttcagacggc tgatccatgc ccccccggtc cagacaagaa
    13501 ccagcggggt cagcagggcg ccggtgggta taatcaccca cccgaacacc ggcacaaaag
    13561 gaagtgacca cagcagaccg gtgaagaacc cggtcagtgc agccagcccc agttccgggg
    13621 tggtgaaccc cctgaacacc gtcggttcgg cgttcagacg gtcgggcaga aaacgaatgg
    13681 tctccataac cagctcccac tgttgcttac aggatgatgg acgcggattt accaagcagc
    13741 cagatgaccg cgaccagcag ta
//
LOCUS       DAZFQD010000140        13485 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.140, whole genome shotgun sequence.
ACCESSION   DAZFQD010000140 DAZFQD010000000
VERSION     DAZFQD010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13485
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.140"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            457..1170
                     /gene="aphA"
                     /locus_tag="ACHKTW_004163"
     CDS             457..1170
                     /gene="aphA"
                     /locus_tag="ACHKTW_004163"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003993 - acid phosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HFO6465083.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPVT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
     gene            1281..1697
                     /gene="yjbQ"
                     /locus_tag="ACHKTW_004164"
     CDS             1281..1697
                     /gene="yjbQ"
                     /locus_tag="ACHKTW_004164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HFO6465084.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMERFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PVHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            1701..2057
                     /gene="yjbR"
                     /locus_tag="ACHKTW_004165"
     CDS             1701..2057
                     /gene="yjbR"
                     /locus_tag="ACHKTW_004165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFO6465085.1"
                     /translation="MTISELLQYCMAKPGAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(2092..4914)
                     /gene="uvrA"
                     /locus_tag="ACHKTW_004166"
     CDS             complement(2092..4914)
                     /gene="uvrA"
                     /locus_tag="ACHKTW_004166"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFO6465086.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            5168..5704
                     /gene="ssb1"
                     /locus_tag="ACHKTW_004167"
     CDS             5168..5704
                     /gene="ssb1"
                     /locus_tag="ACHKTW_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HFO6465087.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            complement(5803..6084)
                     /gene="yjcB"
                     /locus_tag="ACHKTW_004168"
     CDS             complement(5803..6084)
                     /gene="yjcB"
                     /locus_tag="ACHKTW_004168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
                     /protein_id="HFO6465088.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRSDYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIESVMIEEMNQTPPQWPMILT"
     gene            6514..8100
                     /gene="pdeC"
                     /locus_tag="ACHKTW_004169"
     CDS             6514..8100
                     /gene="pdeC"
                     /locus_tag="ACHKTW_004169"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HFO6465089.1"
                     /translation="MSHRARHQLLALPGIIFLVLFPIILSLWIAFLWAKSEVNNQLRT
                     FAQLALDKSELVIRQADLVSDAAERYQGQVCTPAHQKRMLNIIRGYLYINELIYARDN
                     HFLCSSLIAPVNGYTIAPADYKREPNVSIYYYRDTPFFSGYKMTYMQRGNYVAVINPL
                     FWSEVMSDDPTLQWGVYDTVTKTFFSLSKEASAATFSPLIHLKDLTVQRNGYLYATVY
                     STKRPIAAIVATSYQRLITHFYNHLIFALPAGILGSLVLLLLWLRIRQNYLSPKRKLQ
                     RALEKHQLCLYYQPIIDIKTEKCIGAEALLRWLGEQGQIMNPAEFIPLAEKEGMIEQI
                     TDYVIDNVFRDLGDYLATHADRYVSINLSASDFHTSRLIARINQKTEQYAVRPQQIKF
                     EVTEHAFLDVDKMTPIILAFRQAGYEVAIDDFGIGYSNLHNLKSLNVDILKIDKSFVE
                     TLTTHKTSHLIAEHIIELAHSLGLKTIAEGVETEEQVNWLRKRGVRYCQGWFFAKAMP
                     PQVFMQWMEQLPARELTRGQ"
     gene            complement(8103..8426)
                     /gene="soxS"
                     /locus_tag="ACHKTW_004170"
     CDS             complement(8103..8426)
                     /gene="soxS"
                     /locus_tag="ACHKTW_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HFO6465090.1"
                     /translation="MSHQKIIQDLIAWIDEHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            8512..8976
                     /gene="soxR"
                     /locus_tag="ACHKTW_004171"
     CDS             8512..8976
                     /gene="soxR"
                     /locus_tag="ACHKTW_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HFO6465091.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLITSIRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            9522..10871
                     /gene="ghxP"
                     /locus_tag="ACHKTW_004172"
                     /gene_synonym="yjcD"
     CDS             9522..10871
                     /gene="ghxP"
                     /locus_tag="ACHKTW_004172"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HFO6465092.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            11023..12672
                     /gene="yjcE"
                     /locus_tag="ACHKTW_004173"
     CDS             11023..12672
                     /gene="yjcE"
                     /locus_tag="ACHKTW_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HFO6465093.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(12826..>13485)
                     /locus_tag="ACHKTW_004174"
     CDS             complement(12826..>13485)
                     /locus_tag="ACHKTW_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFO6465094.1"
                     /translation="IKMADGTLNACSAMHVQFYNAAMNRANIKNTFLDYSNFYMAYMA
                     EVNLYKVIAPYVNLFKADLSFSKLDLINFEHADLSRVNLNKAILQNINLIDSKLFCTW
                     LTNTFLEMVICTGSNMANVNFNNANLSNCHFNCSILTKACMFNTRLYRVNFDEASVQG
                     MGISILRGEENIPIDSDTLVTLQKFFEEDCTSHTGMSQTEDNINAVAMKITADIMQHA
                     D"
BASE COUNT         3195 a         3267 c         3496 g         3527 t
ORIGIN      
        1 ctagctcaac aggtaaagat gtcgttttgt tgctattcac atatgatata ttcatatcat
       61 catctccatc gggagtaata aaattagtca ccattgtagg atagggttct ggtcaatcag
      121 gaatcatctt tataaacttc gattgttttt gtaatgctgt atcattaagt tcattaaatc
      181 gtacagcaga taaatgttct atcaaatttc gctcatttgc agaggattca tcataataaa
      241 cgtaaaatta atgtatcctt acatcgagta ataaacattt tttatacaaa aaaagacagg
      301 aacgtattta ctgggttaaa cataatcatc ctgcttttca tcacaaaaac cgcagataat
      361 ccttcctttc cccggcagct ggcgttatgg tcagatggtt tttgcaacaa atctcacaat
      421 aaaaagtttc aacatactga ctatttaggg aaaaatatgc gcaagatcac acaggcaatc
      481 agtgccgttt gcttattgtt cgctctaaac agttccgctg ttgccctggc ctcatctcct
      541 tcaccgctta accctgggac taacgttgcc aggcttgctg aacaggcacc aattcattgg
      601 gtttcggtcg cacaaattga aaatagcctc gcagggcgtc cgccaatggc ggtggggttt
      661 gatatcgatg acacggtact tttttccagc ccgggcttct ggcgcggcaa aaaaaccttc
      721 tcaccagaaa gcgaagatta tctgaaaaat cctgtgttct gggaaaaaat gaacaatggc
      781 tgggatgaat tcagcattcc aaaagaggtc gctcgccagc tgattgatat gcatgtacgc
      841 cgcggtgacg cgatcttctt tgtgactggt cgtagcccga cgaaaacaga aacggtttca
      901 aaaacgctgg cggataattt tcatattcct gtcaccaaca tgaatccggt gatctttgcg
      961 ggcgataaac cagggcaaaa tacaaaatcg caatggctgc aggataaaaa tatccgaatt
     1021 ttttatggcg attccgataa cgatattacc gccgcacgcg atgtcggcgc tcgtggtatc
     1081 cgtattctgc gcgcctccaa ctctacctac aaacccttgc cacaagcggg tgcgtttggt
     1141 gaagaggtga tcgtcaattc agaatactga cagagcggga gagcgtgacg ctctcccgaa
     1201 tgctgttttt ttaatcacac ctttatcctt tcgctgtctt gctgcaaact gattaagaga
     1261 gttttatcaa ggagcagcac atgtggtatc aaaagacgct cacgcttagc gccaaatctc
     1321 gtgggtttca tctggtaacg gatgaaattc tgaatcagct ggctgatatg ccgcgcgtta
     1381 acatcggctt actgcatctg ttgctgcaac atacctccgc ctctctgaca cttaatgaga
     1441 actgcgatcc caccgtacgc cacgacatgg agcgtttttt cctccgcacc gttcccgaca
     1501 acggaaatta tgagcatgac tatgagggag cagacgatat gccttctcat atcaaatcct
     1561 caatgctggg aacatcgctt gtattgccgg tacataaagg gcgtattcag accggcacct
     1621 ggcaaggcat ctggctgggg gaacatcgca tccacggcgg atcgcgtcgc atcatcgcga
     1681 cactacaagg ggagtaaaaa atgaccattt cggagttgct acaatattgc atggcaaaac
     1741 caggcgcaga acagagcgtg cataacgact ggaaagcgac gcagatcaaa gtggaagatg
     1801 tactgtttgc gatggtgaaa gaagtagaaa atcgcccagc tgtttcgctg aaaaccagcc
     1861 cggagctggc ggagctgcta cgtcagcagc acagcgatgt gcgtcctagc cgccatctga
     1921 ataaagcgca ctggagcacc gtgtatctcg acggttcgct gccggattcg caaatctatt
     1981 atctggtgga tgcgtcttat cagcaggcag tgaatttact accggaagaa aagcgtaaat
     2041 tgctggtaca actctgaaag gaaaaggccg ctcagaaagc ggccttaacg attacagcat
     2101 cggcttgagg aagcgtgccg tatgcgaagc ttcgcactcc gcgacggttt ctggcgtacc
     2161 ggagacgagg atttcgccgc cgccactgcc gccttctggt cccaggtcga caatccagtc
     2221 agcggttttg atcacgtcga gattgtgctc aatcaccaca atggtattgc cctgatcgcg
     2281 cagtttatgc agcacgtcga ggagttgctg aatatcggcg aagtgcagac cggtggtcgg
     2341 ctcgtcgaga atatacagcg tctgcccggt gccgcgtttt gacagctcac gcgccagctt
     2401 cacgcgctgg gcttcaccac cagaaagtgt ggttgcggac tgccccaggc gaatgtacgt
     2461 cagaccaacg tccatcaacg tttgcagctt acgcgccaga gctggcaccg catcaaagaa
     2521 ctcacgcgcc tcttcgatgg tcatatccag cacttcgtgg atggttttgc ctttgtactt
     2581 aatttccagc gtttcacggt tatagcgttt acctttgcac tgatcgcacg gcacgtagat
     2641 atccggcagg aagtgcatct ccactttgat cacaccgtcg ccctgacagg cttcgcagcg
     2701 cccgccacgg acgttaaagc tgaaacgtcc tggcgtataa ccacgcgcac gggattccgg
     2761 tacgcccgca aacagttcgc gcacaggtgt aaacacgccg gtataggtcg ccgggttaga
     2821 acgtggagta cgaccaattg ggctttggtc gatatcgatc actttatcga aatgctccag
     2881 cccctgaata tcgcgatacg gtgccggttc ggcgatggtc gcaccattca actggcgttg
     2941 ggcaatcggg aacagtgtgt cgttaatcag cgtcgattta ccggaacctg aaacccctgt
     3001 gatgcaggta aacagaccga ctggcagcgt gagcgtcacg tccttcaggt tgttgccgcg
     3061 tgcgcctgtc agcttcagca ctttttccgg atttgccgga acgcgtttct tcggcacttc
     3121 aatcttgcgt ttaccgctca tgtactgccc ggtcaacgac tccggcaccg ccataatcgc
     3181 ttccagcgga ccttctgcga ccacttcacc gccgtgaaca cctgcgcccg ggccaatgtc
     3241 gatcacatgg tcagcggcgc gaattgcgtc ttcgtcgtgc tccaccacaa tcacggtatt
     3301 accgagatcg cgcagatgga taagcgtacc caacaggcgc tcgttatcgc gctggtgcag
     3361 gccgatagat ggctcgtcca gcacgtacat aacgccaacc aggcccgcac caatctggct
     3421 cgccagacgg atacgctggg cttcaccgcc ggaaagcgtt tctgccgagc gggaaagcgt
     3481 caggtaattc aggccgacgt taacgaggaa tttcaaacga tcgccgatct ctttaaggat
     3541 tttttccgca atcttcgccc gttgaccagc gagtttgaga ttgttgaaga attccatcgc
     3601 atgaccgatg ctcatgtcgg agatagcagg cagcggcgta ttctcgacat aaacgtggcg
     3661 cgcttcccga cgcagacgcg tcccttcgca gctggcgcat gggcgattgc tgataaactt
     3721 ggctaattct tcacgtaccg cactggattc tgtctcttta taacggcgct ccatattgtg
     3781 cagcacgcct tcgaacggat gacgacggat ggaggtatcg ccacgatcgt tcatgtattt
     3841 gaattcaatg ttttctttgc cagaaccgta caacaccact ttatgcacgt tcgcgctcag
     3901 gctgccccac ggcgcttcga cgtcgaactt atagtgatct gccagcgatt tcagcatctg
     3961 gaagtaatag aagttgcggc gatcccagcc acggatcgca ccgccagcca gtgacagctc
     4021 ggggttttgg atcacgcggt caggatcgaa atattgctgt acgccaaggc cgtcacaggt
     4081 tgggcaggca cctgccgggt tgttaaacga aaacagtcgc ggctccagtt cacgcatact
     4141 gtagccgcaa attgggcagg cgaagttggc ggagaacagc agctcttccg ctttcgggtc
     4201 gtccatatcc gccactaccg ctgtaccacc ggaaagctcc agcgcggttt caaacgactc
     4261 cgcaagacgt tgggtaagat cgtcacgcac cttgaagcga tcaaccacca cttcaatggt
     4321 atgtttcttt tgcagttcca gtttcggcgg atcggaaaga tcgcagactt cgccatcaat
     4381 acgagcacgg atgtaaccct ggcttgccag gttctccagc gttttggtgt gttcgccttt
     4441 gcgctcttta atgattggcg cgagcagcat cagacgcttg ccttccggct gcgacagcac
     4501 gttatccacc atctggctga cggtttgcgc cgccagcggg acgtcgtggt ccggacagcg
     4561 cggttcgccg acgcgggcga acaacaaacg caaatagtcg tggatttcgg tgattgtccc
     4621 caccgtagaa cgcgggttat gagacgtcga tttctgctca attgagatgg caggagaaag
     4681 cccctcaata tgatcgacgt ccggcttttc catcagtgac agaaactgcc gcgcgtaggc
     4741 ggaaagggat tcaacgtaac ggcgctgccc ttcggcatat aaggtgtcga aagcgagcga
     4801 ggatttgcca gaacccgaaa gcccggtcac gacaatgagc ttgtcgcggg ggataacgag
     4861 gttgatgttt ttgagattat gggtgcgggc gccccgaact tcgatcttat ccattcacct
     4921 ttcccggatt aaacgctttt ttgcccggtg gcatggtgct accggcgatc acaaacggtt
     4981 aattatgaca caaattgacc tgaatgaata tacagtattg gaatgcatta cccggagtgt
     5041 tgtgtaacaa tgtctggcca ggtttgtttc ccggaaccga ggtcacaaca tagtaaaagc
     5101 gctattggta atggtacaat cgcgcgttta cacttattca gaacgatttt tttcaggaga
     5161 cacgaacatg gccagcagag gcgtaaacaa ggttattctc gttggtaatc tgggtcagga
     5221 cccggaagta cgctacatgc caaatggtgg cgcagttgcc aacattacgc tggctacttc
     5281 cgaatcctgg cgtgataaag cgaccggcga gatgaaagaa cagactgaat ggcaccgcgt
     5341 tgtgctgttc ggcaaactgg cagaagtggc cagcgaatat ctgcgtaaag gttctcaggt
     5401 ttatatcgaa ggtcagctgc gtacccgtaa atggaccgat caatccggtc aggatcgcta
     5461 caccacagaa gtcgtggtga acgttggcgg caccatgcag atgctgggtg gtcgtcaggg
     5521 tggtggcgct ccggcaggtg gtaatatcgg tggtggtcag ccgcagggcg gttggggtca
     5581 gcctcagcag ccgcagggtg gcaatcagtt cagcggcggc gcgcagtctc gcccgcagca
     5641 gtccgctccg gcagcgccgt ctaacgagcc gccgatggac tttgatgatg acattccgtt
     5701 ctgatttgtc attaaaacaa taggttatat tgttttaagg tggatgatta aagcatctgc
     5761 cagccataaa aaagaagcct ccgttatgga ggcttctacg tatcaggtca aaatcattgg
     5821 ccattgtggc ggtgtctgat tcatctcttc tatcatcacc gactcgatgt tgtgccagat
     5881 agcggtgatg tccgccattg tgatgccaag caaccccatt gcgaaccaac aggcggaaac
     5941 aacgcccaga ccgctgctga tcaccgcaag cccgacataa tcagaccgac gaaaacggat
     6001 gttgagtgtg ctggccagaa acatcattac ggcactaaga agttgccagc gaagaagaac
     6061 cacgccagtg gtgagggtag ccatcaaaca attcctctga aaagagccga tgccctggac
     6121 agcgcggcgt tgttcacggg aggcgtggta cactctggct atcgcggggc ttgcagaaca
     6181 caaaaatgaa acactctgtt tgtttcatta attttgtgaa ctatatcaca attgattgtt
     6241 tgttagccat attaggccgt gacttttatt gctgtacaga ttatgtggtt tttcagtggc
     6301 attaagggca tatcttcccg ccgcctctgc attcctgtag gaaattaatt ttgaatatca
     6361 atgaattatt ttcatccagg tgacgattag aaaggtatca atttcaaatc aggcaaaagt
     6421 gctatttata ccgtaagatt tatctaaaga cgtcggtacc cagggttttc accttgcaat
     6481 ggccgggtat aaacaggcag gaaattgata gcaatgagtc atcgtgcacg acaccaatta
     6541 ctggcgttgc cgggcattat ctttttagtt ctctttccca tcattctttc gctatggatt
     6601 gccttccttt gggcaaaatc agaagtgaat aatcagctcc gaacctttgc tcaactggca
     6661 ctggataaat ccgagctggt cattcgccag gcagatttag tgagcgatgc agctgaacgc
     6721 tatcaggggc aagtttgcac tccagcccat caaaagcgaa tgttgaatat tattcgtggc
     6781 tatctttata ttaatgaatt gatctatgcc cgtgataacc attttttatg ctcatcgctg
     6841 atagcgcctg taaacggcta tacgattgca ccggccgatt ataagcgtga acctaacgtt
     6901 tctatctatt attaccgcga tacgcctttt ttctctggct ataaaatgac ctatatgcag
     6961 cggggaaatt atgtggcggt tatcaaccct ctcttctgga gtgaagtgat gtctgatgac
     7021 ccgacattgc aatggggtgt gtatgatacg gtgacgaaaa cctttttctc gttaagcaaa
     7081 gaggcctcgg cagcaacgtt ttctccgctg attcatttga aggatttaac cgtacaaaga
     7141 aatggctatt tatatgcgac agtttattcg acaaaacgcc caattgcagc cattgttgcg
     7201 acttcatatc aacgtcttat aacccatttt tataatcatc ttatttttgc gttgcccgcc
     7261 ggtattttgg ggagtcttgt tctgctatta ctctggctac gtattcgaca aaactattta
     7321 tctcccaaac gtaaattgca acgcgccctc gaaaaacatc aactttgtct ttattaccag
     7381 ccaataatcg atatcaaaac agaaaaatgt atcggcgctg aagcgttgtt acgttggctt
     7441 ggtgagcagg ggcaaataat gaatccggca gagtttattc cgctggcaga aaaggagggg
     7501 atgatagaac agataactga ttatgttatt gataatgtct tccgcgatct gggcgattac
     7561 ctggcaacac atgcagatcg ctatgtttct attaacctgt cggcctccga ttttcatacg
     7621 tcacggttga tagcgcgaat caatcagaaa acagagcaat acgcggtgcg tccgcagcaa
     7681 attaaatttg aagtgactga acatgcattt cttgatgttg acaaaatgac gccgattatt
     7741 ctggctttcc gccaggcagg ttacgaagtg gcaattgatg attttggtat tggctactct
     7801 aacttgcata accttaaatc attgaatgtc gatattttga aaatcgataa atcgtttgtt
     7861 gaaacgctga ccacccacaa aaccagtcat ttgattgcgg aacacatcat cgagctggcg
     7921 cacagcctgg ggttaaaaac gatcgctgaa ggcgtcgaaa ctgaggagca ggttaactgg
     7981 ctgcgcaaac gcggcgtgcg ctattgccag ggatggtttt ttgcgaaggc gatgccgccg
     8041 caggtgttta tgcaatggat ggagcaatta cccgcgcggg agttaacgcg cgggcaataa
     8101 aactacaggc ggtggcgata atcgctggga gtgcgatcaa actgccgacg gaaaacgcgg
     8161 gagaaggtct gctgcgagac ataacccagg tccattgcga tatcaaaaat cggacgctcg
     8221 gtggtgcgca actcaacggc ggccagtaac aggcgacgtt ggcgaatgta atcgccaagc
     8281 gtctgatgcg tcaccgtgcg gaacattcgt tgcaagtacc actttgaata gcctgatttt
     8341 tttgcgacta catcaatgtt aagcggctgg tcaatatgct cgtcaatcca tgcgataaga
     8401 tcctgaataa ttttctgatg ggacataaat ctgcctcttt tcagtgttca gttcgttaat
     8461 tcatctgttg gggagtataa ttcctcaagt taacttgagg taaagcgatt tatggaaaag
     8521 aaattacccc gcattaaagc gctgctaacc cccggcgaag tggcgaaacg cagcggtgtg
     8581 gcggtatcgg cgctgcattt ctatgaaagt aaagggttga ttaccagtat ccgtaacagc
     8641 ggcaatcagc ggcgatataa acgtgatgtg ttgcgatatg ttgcaattat caaaattgct
     8701 cagcgtattg gcattccgct ggcgaccatt ggtgaagcgt ttggcgtgtt gcccgaaggg
     8761 catacgttaa gtgcgaaaga gtggaaacag ctttcgtccc aatggcgaga agagttggat
     8821 cggcgcattc ataccttagt ggcgctgcgt gatgaactgg acggatgtat tggttgtggc
     8881 tgcctttcgc gcagtgattg cccgttgcgt aacccgggcg accgcttagg agaagaaggt
     8941 accggcgcac gcttgctgga agatgaacaa aactaaagcg ccacaagggc gctttagttt
     9001 gttttccggt ctttgtcttt ctctctatcc cgctggtaca caggagggtt tcccccgacg
     9061 tcaacacacc tcattcgagc acgtggtgga ggttccggtt ggtgttgatg ctttaattgt
     9121 atgtcaccga cgtttcttcg ccagtgtaaa agtatacttt ttaaccgcaa tatttttgtc
     9181 atctcagacg attttttatc gcaatcctga acggtatacg gctcgataac gctgcaatct
     9241 tgcgcaccga cgataacgtt tgcgcatcaa ttgcctgttt tttcatcgtc aagacaataa
     9301 aagagaaaaa agcagcaaac ttcggttgaa aaagccgcta tgatcgccgg ataatcgttt
     9361 gcttttttta ccacccgttt tgtatgcgcg gagctaaacg tttgcttttt tgcgacgcag
     9421 caaattgtcg caaacctgga gcaggaagat aacgtttcgc tggcagggga ttgtccgcca
     9481 cgcatcttga cgaaaattaa actctcaggg gatgttttct tatgtctacg ccatcagcgc
     9541 gtaccggcgg ttcactcgac gcctggttta aaatttcaca acgtggaagc actgtccgtc
     9601 aggaagtggt tgccgggtta acaacgtttc tggcgatggt ctactcggtc atcgtcgttc
     9661 caggtatgtt gggtaaagca ggcttcccgc ctgcggcagt tttcgttgca acctgtctgg
     9721 ttgccggact cggttctatc gtgatgggtc tgtgggctaa tctgccgttg gcgattggtt
     9781 gcgccatctc cctgacggcg tttaccgcat tcagcctggt gctggggcaa catattagcg
     9841 tacctgtcgc gctgggtgcc gtgttcctga tgggtgtgct gtttacggta atttctgcca
     9901 cgggtatccg tagctggatt ttgcgcaact tgcctcacgg tgtggcgcac ggcacgggga
     9961 tcggtatcgg tctgttcctg ctgctcattg ccgctaatgg tgtcggtctg gtgattaaaa
    10021 acccgcttga tggtctgccc gttgcgctgg gtgatttcgc gaccttcccg gtgattatgt
    10081 cactggtagg tctggcggtg atcatcggcc tggaaaaact gaaagtccct ggtggcattc
    10141 tgctgaccat tatcggtatc tcaattgtcg gtttgatctt cgatcctaac gtccatttct
    10201 ccggcgtttt cgccatgcct tcattgagcg atgaaaacgg caattcactg attggcagcc
    10261 tggacattat gggcgcgctg aatcctgtag tcctgccaag cgttctggcg ctggtgatga
    10321 cggcagtatt tgatgccacc ggaactatcc gtgccgtcgc cggccaggcg aacctgctgg
    10381 ataaagatgg gcagatcatc gacggtggga aagcactgac cactgactcc atgagcagcg
    10441 ttttctctgg cctggtgggt gctgctccgg cagcggtata catcgagtct gcggcgggta
    10501 cggcggcggg cggtaaaacc ggtttgacgg ctatcaccgt tggcgtgctg ttcctgctga
    10561 ttctgttcct ctctccgctc tcttacctcg ttccggggta tgcaacggct ccggcgctga
    10621 tgtacgttgg cctgctgatg ctgagcaacg tggcgaaaat cgactttgct gattttgttg
    10681 atgcgatggc gggtctggtt acggcggtat tcatcgtgct gacctgtaac atcgtaacag
    10741 gcatcatgat cggcttcgcg actctggtga ttggtcgtct ggtttccggc gaatggcgca
    10801 agttgaacat cggtacggtc gttatcgccg tggcgctggt gaccttctat gcgggtggct
    10861 gggctatcta atctttcatc ggattctgaa aacgggtggc aatggctgcc cgtttttatt
    10921 ttctccgcac attgtgtgag cttttgcgat attctgaaaa aatgagaatt caggcataac
    10981 gtctttctca gatcataaaa aaacatcgca aacagggaac gcatggaaat cttcttcacc
    11041 atactgataa tgaccctcgt ggtctcgctg tccggggtgg tcactcgtgt catgcccttt
    11101 cagatcccgc ttccgcttat gcaaatcgcc atcggtgcgc tactggcgtg gccgacgttt
    11161 ggtttgcatg tggagtttga tcctgaactc tttttagtct tgtttatccc gccgttgctg
    11221 ttcgctgatg gctggaaaac gccgacccgt gaatttctcg aacatggtcg agagattttt
    11281 ggcctcgcgc tggcgctggt ggtggtcacc gtggtcggca ttggcttcct tatttactgg
    11341 gtggtgccgg gcattccgct gatccccgcc tttgcgctgg cggcagtgct ttctccgacc
    11401 gatgctgtgg cgctctccgg gattgttggc gaagggcgca tcccgaaaaa aatcatgggt
    11461 attttgcagg gcgaagcgtt gatgaatgac gcctccggcc tggtgtcgtt gaagtttgcc
    11521 gtggcagtgg cgatggggac gatgatcttc accgttggcg gtgcgacggt cgaatttatg
    11581 aaagtagcca ttggcggtat tctcgccggc tttgtggtga gctggctgta tggtcgctcg
    11641 ctgcgattcc tcagccgctg gggcggtgat gaacccgcga cgcagatcgt tctgctgttc
    11701 ttgctgccat tcgcttctta tctgattgcc gaacatattg gcgtttccgg catcctcgct
    11761 gcggttgccg ccgggatgac catcacccgc tccggtgtga tgcgccgtgc gccgctggca
    11821 atgcgcctgc gcgcaaacag cacctgggcg atgctggaat ttgtctttaa cggcatggtg
    11881 ttcctgctgt taggtctgca gctgccgggt attctggaga cgtcgctgat ggcggcagaa
    11941 atcgacccta acgtcgaaat ctggatgctg tttaccgata ttattctgat atatgcggcg
    12001 ctgatgctgg tccgtttcgg ctggctgtgg acgatgaaaa agttcagcaa ccgcttcctg
    12061 aagaagaagc cgatggagtt tggttcgtgg accacacgag aaatcctgat cgcgtctttc
    12121 gccggggtgc gtggggcgat cactctggcc ggtgtgctct ctatcccgct gctcttgccg
    12181 gatggtaacg tcttcccggc gcgctatgag ctggtgttcc tggcggctgg cgtcattctc
    12241 ttctcgctgt ttgtcggcgt ggtgatgttg cctattctgc tacaacacat tgaagtcgcg
    12301 gaccattcgc aacaattgaa agaagaacgt attgcgcgag cggcaacggc agaagtggcg
    12361 attgtggcga tccagaaaat ggaggagcgt ctggcggcgg ataccgaaga gaatatcgat
    12421 aaccagctgc ttacggaggt cagttctcgc gtcattggta acctgcgtcg tcgcgccgat
    12481 ggacgtaacg acgttgaaag ttccgtgcag gaagagaacc ttgagcgtcg cttccgtctg
    12541 gcggcattgc gttctgaacg tgctgaactg taccacctgc gcgccacgcg ggagatcagc
    12601 aacgaaacgc tgcaaaaatt actgcacgat ctcgatttgc ttgaagcgtt gctaattgag
    12661 gaaaatcagt aagccgaaag cgacgtaacc aggcccgagg taatctgaaa aaggtgcaat
    12721 cttttgcacc tttaacaggt tagctcgggc taacattcat gattctaaaa caaataagtt
    12781 atctctcttg taaagtcaat tcattggctg ttcttcgact taacctcaat ctgcgtgttg
    12841 cataatatct gcagtaatct tcatagcgac tgcattaata ttatcctcag tttgtgacat
    12901 gccagtatga gaggtacaat cttcttcaaa gaatttctgt agtgttacca gggtatcact
    12961 atcaatgggg atattttcct ccccacggag aatagaaatg cccattccct ggacgctagc
    13021 ctcatcaaaa ttaacccgat agagacgggt attaaacata caggcttttg ttaaaataga
    13081 acagttgaaa tggcagttgc ttaaattggc attattaaaa ttaacattag ccatattaga
    13141 gccggtacat ataaccattt cgaggaatgt atttgttagc cacgtacaaa agagtttgct
    13201 atcaattaag tttatattct ggaggattgc tttgttcaga ttgacgcgag acagatcagc
    13261 atgttcaaag ttaattaaat cgagtttaga gaaactaagg tcggctttaa ataaattaac
    13321 atatggcgct attactttat aaagatttac ctcagccatg tacgccatat aaaaatttga
    13381 atagtcaaga aaggtatttt taatattggc tctattcatt gctgcattgt agaattgaac
    13441 atgcatagcg gagcatgcat ttagagttcc gtcggccatt tttat
//
LOCUS       DAZFQD010000141        13295 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.141, whole genome shotgun sequence.
ACCESSION   DAZFQD010000141 DAZFQD010000000
VERSION     DAZFQD010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13295
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.141"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..221)
                     /locus_tag="ACHKTW_004175"
     CDS             complement(<1..221)
                     /locus_tag="ACHKTW_004175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6465095.1"
                     /translation="MPEQAITKALCIYQGQQINLAYIRLRHFEFTNGRIISDFNGMGK
                     VKYHFLTIKRVLFLVYQTISIHSKPLEII"
     gene            158..1657
                     /gene="pitB"
                     /locus_tag="ACHKTW_004176"
     CDS             158..1657
                     /gene="pitB"
                     /locus_tag="ACHKTW_004176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HFO6465096.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPTDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRYWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLIAMEPPLPAASTDGMQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
     gene            complement(1706..2398)
                     /gene="yghT"
                     /locus_tag="ACHKTW_004177"
     CDS             complement(1706..2398)
                     /gene="yghT"
                     /locus_tag="ACHKTW_004177"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006235 - dTTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFO6465097.1"
                     /translation="MQSITPPLIAVIGSDGSGKSTVCEHLITVVEKYGAAERVHLGKQ
                     AGNVGRAVTKLPLMGKSLHKTIERNQVKTAKKLPGPVPALVITAFVARRLLRFRHMLA
                     CRRRGLIVLTDRYPQDQIPGAYDGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDL
                     VIKLNVDLEVACARKPDHKRESLARKIAITPQLTFGGAQLVDIDANQPLEQVLVDAEK
                     AITDFMTARGYH"
     gene            2572..3285
                     /gene="yghS"
                     /locus_tag="ACHKTW_004178"
     CDS             2572..3285
                     /gene="yghS"
                     /locus_tag="ACHKTW_004178"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006235 - dTTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFO6465098.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSTLTASLVNELAARMPA
                     EHIYLGQSSGRIGEWISQLPVIGAPFGRYLRSKAAHVHEKPSTPPGNITALVIYLLSC
                     WRAYKFRKMLCKSQQGYLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGARILELDGR
                     QPADEILQASLRAIHAALS"
     gene            3317..4075
                     /locus_tag="ACHKTW_004179"
     CDS             3317..4075
                     /locus_tag="ACHKTW_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6465099.1"
                     /translation="MDALQTQTVISTTAPQPSYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCFSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMF
                     LAQRERRLYQQMAQYRPELIIRLGIDIETAISRKPDHDYAELQDKIGVMSKIGYNGTK
                     ILEIDSRAPYSEVLEQAQKAVSLVAIVSDRRSLT"
     gene            4121..5470
                     /locus_tag="ACHKTW_004180"
     CDS             4121..5470
                     /locus_tag="ACHKTW_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HFO6465100.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVVSALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVVAWYFDFGFAGFVIAWYVSNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVFI
                     VGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVALSLIPTLKAFFQRHSLLYNAAGEKS"
     gene            5470..6294
                     /locus_tag="ACHKTW_004181"
     CDS             5470..6294
                     /locus_tag="ACHKTW_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6465101.1"
                     /translation="MMRKYFPLEASERLFVAIEEDDVVDAQVSLPPTIALSCTTEIIH
                     DNYALCLQFWLNGVDRQELLRLVRKQAKGDELTADERKQFKYMRARYKHLRFAQRLYL
                     KKHQAGFLFGKTTVFLGRFQDGFRNGKKNIVSYYGNLLRIYLSSPVWSLVNYSLRHSQ
                     LESVSSFIAYRQKQMHTLKEIIAKPRLTGREFHDVRKIISQQVSYYDTLRSLDPENKE
                     ALQISRFLAAINGLMGDKHDDMVADDMENRQSYDAPLALDSDIRQRLELLISRFPL"
     gene            6306..6866
                     /locus_tag="ACHKTW_004182"
     CDS             6306..6866
                     /locus_tag="ACHKTW_004182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HFO6465102.1"
                     /translation="MSSRLIIALIIMLLAPGVQAHNFVTGKTVTPVYIQEGGELLLNS
                     DDEIHYQKWNSTQLAGKVRIIQYIAGRKSAKKKNSLLIKAVEAANFPQDRFQPTTIVN
                     TDDAIFGTGYFVVGKIEKNKRRYPWAQFVIDGNGQGRVAWRLPEQSSTILVLNKAGQI
                     QWAKDGSLTPEEVDHVIALAQKLINE"
     gene            6897..7967
                     /gene="lptF"
                     /locus_tag="ACHKTW_004183"
     CDS             6897..7967
                     /gene="lptF"
                     /locus_tag="ACHKTW_004183"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HFO6465103.1"
                     /translation="MKLVEHYIMRGTRRLVLIIVGFLIFIFASYSAQRYLTEAANGTL
                     ALDVVLDIVFYKVLIALEMLLPVGLYVSVGVTLGQMYTDSEITAISAAGGSPGRLYKA
                     VLYLAIPLSIFVTLLSMYGRPWAYAQIYQLEQQSQSELDVRQLRAKKFNTNDNGRMIL
                     SQTVDQDNNRLTDALIYTSTANRTRIFRARSVDVVDPSPEKPTVMLHNGTAYLLDHQG
                     RDDNEQIYRNLQLHLNPLDQSPNVKRKAKSVTELARSAFPADHAELQWRQSRGLTALL
                     MALLAISLSRVKPRQGRFSTLLPLTLLFIAIFYGGDVCRTLVANGAIPLIPGLWLVPG
                     LMLMGLLMLVARDFSLLQKFSR"
     gene            7964..9043
                     /gene="lptG"
                     /locus_tag="ACHKTW_004184"
     CDS             7964..9043
                     /gene="lptG"
                     /locus_tag="ACHKTW_004184"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HFO6465104.1"
                     /translation="MNVFSRYLIRHLFLGFAAAAGLLLPLFTTFNLINELDDVSPGGY
                     RWTQAVLVVLMTLPRTLVELSPFIALLGGIVGLGQLSKNSELTAIRSTGFSIFRIALV
                     ALVAGILWTVSLGAIDEWVASPLQQQALQIKSTATALGEDDDITGNMLWARRGNEFVT
                     VKSLNEQGQPVGVEIFHYRDDLSLESYIYARSATIKDDKTWILHGVNHKKWLNGKETL
                     ETSDNLAWQSAFTSMNLEELSVPGNTFSVRQLNHYIHYLQETGQPSSEYRLALWEKLG
                     QPILTLAMILLAVPFTFTAPRSPGMGSRLAVGVIVGLLTWISYQIMVNLGLLFALSAP
                     VTALGLPVAFVLVALSLVYWYDRQH"
     gene            complement(9079..10251)
                     /locus_tag="ACHKTW_004185"
     CDS             complement(9079..10251)
                     /locus_tag="ACHKTW_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFO6465105.1"
                     /translation="MGLYDKYARLAGERLQFSDNGLTPFGTCIDEVYSATEGRIGNKK
                     VILAGTNNYLGLTFNHDAIAEGQAALAAQGTGTTGSRMANGSYAPHLALEKEIAEFFK
                     RPTAIVFSTGYTANLGVISALADHNAVVLLDADSHASIYDACSLGGAEIIRFRHNDAK
                     DLERRMVRLGERAKEAIIIVEGIYSMLGDVAPLAEIVDIKRRLGGYLIVDEAHSFGVL
                     GATGRGLAEAVGVEDDVDIIVGTFSKSLASIGGFAVGSEAMEVLRYGSRPYIFTASPS
                     PSCIATVRSSLRTIATQPELRQKLMDNANHLYDGLQKLGYELSSHISPVVPVIIGSKE
                     EGLRIWRELISLGVYVNLILPPAAPAGITLLRCSVNAAHSREQIDAIIQAFATLKQ"
     gene            complement(10251..10499)
                     /locus_tag="ACHKTW_004186"
     CDS             complement(10251..10499)
                     /locus_tag="ACHKTW_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFO6465106.1"
                     /translation="MVNREIVMDYILSCLQDLVENGVEIKPDSDLVNDLGLESIKVMD
                     LLMMLEDRFDISIPINILLDVKTPAQLMETLLPWLENK"
     gene            complement(10531..11445)
                     /locus_tag="ACHKTW_004187"
     CDS             complement(10531..11445)
                     /locus_tag="ACHKTW_004187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001481799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFO6465107.1"
                     /translation="MNQTVAVTGATGFIGKYIIDNLLARGFHVRALTRTARAHVNDNL
                     TWVRGSLEDTHSLSELVAGASAVVHCAGQVRGHKEEIFTHCNVDGSLRLMQAAKESGF
                     CQRFLFISSLAARHPELSWYANSKHVAEQRLTAMADEITLGVFRPTAVYGPGDKELKP
                     LFDWMLRGLLPRLGAPDTQLSFLHVTDFAQAVGQWLSAETVQTQTYELCDGVPGGYDW
                     QHVRQLAADARCGSVRMVGIPLPVLTCLADISTALSRLAGKEPMLTRSKIRELTHADW
                     SASNNRISEDINWFPGISLEQALRNGLF"
     gene            complement(11442..13130)
                     /locus_tag="ACHKTW_004188"
     CDS             complement(11442..13130)
                     /locus_tag="ACHKTW_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001383581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HFO6465108.1"
                     /translation="MRYADFPTLVDALDYAALSSAGMNFYDRRCQLEDQLEYQTLKAR
                     AEAGAKRLLSLNLKKGDRVALIAETSSGFVEAFFACQYAGLVAVPLAIPMGVGQRDSW
                     SAKLQGLLASCQPAAIITGDEWLPLVNAATHNNPELHVLSHAWFKALPEADVALQRPV
                     PNDIAYLQYTSGSTRFPRGVIITHREVMANLRAISHDGIKLRPGDRCVSWLPFYHDMG
                     LVGFLLTPVSTQLSVDYLRTQDFAMRPLQWLKLISKNRGTVSVAPPFGYELCQRRVNE
                     KDLAELDLSCWRVAGIGAEPISAEQLHQFAECFRQVNFDNKTFMPCYGLAENALAVSF
                     SDEASGVVVNEVDRDILEYQGKAVAPGAETRAVSTFVNCGKALPEHGIEIRNEAGMPV
                     AERVVGHICISGPSLMSGYFGDQASQDEIAATGWLDTGDLGYLLDGYLYVTGRIKDLI
                     IIRGRNIWPQDIEYIAEQEPEIHSGDAIAFVTAQEKIILQIQCRISDEERRGQLIHAL
                     AARIQSEFGVTAAIDLLPPHSIPRTSSGKPARAEAKKRYQKAYAASLHVQESLA"
BASE COUNT         3201 a         3274 c         3445 g         3375 t
ORIGIN      
        1 ttgattattt ctaaaggttt tgaatgaatg cttattgtct gatacacaag aaataacact
       61 ctttttatcg ttaaaaaatg atatttcact ttgcccatgc cgttaaagtc actgataatg
      121 cgtccgttcg taaattcaaa atggcgtaat ctaatatatg ctaaatttat ttgttggcct
      181 tgatatatac acagggcttt tgttattgct tgctctggca tttgtgttgt tctacgaagc
      241 aatcaatggt tttcatgaca cggcgaatgc ggtggcaacc gttatttata ctcgtgccat
      301 gcaaccacaa cttgctgtgg tgatggcggc attttttaac ttttttggcg tgttattggg
      361 cggacttagc gttgcctatg ccattgtcca tatgttgcca accgatttgt tgctgaatat
      421 ggggtcaacc cacggcctgg cgatggtctt ttccatgctg ctggcggcga ttatctggaa
      481 cctgggaacg tggttcttcg gtttaccggc ctccagttcg cacaccttga ttggtgcgat
      541 tatcggcatc ggtttaacca acgcgctgtt aaccggctca tcggtgatgg atgcgttaaa
      601 cctgcgtgaa gtgaccaaaa ttttctcctc gctgattgtt tcccctatcg tcggcctggt
      661 cattgcggga ggcctgatat tcctgctgcg acgctactgg agcgggacga aaaagcgtga
      721 ccgtattcac cgcattccgg aagatcgcaa aaagaaaaaa ggcaaacgta aaccgccatt
      781 ctggacgcgt attgcgctga ttgtttccgc tgcgggcgtg gcgttttcgc acggcgcgaa
      841 cgacggacaa aaagggatcg gcctggtgat gctggtactg gtggggattg cccctgctgg
      901 cttcgtcgtc aatatgaacg cgtccggcta tgaaattacc cgtacccgcg atgccgtcac
      961 caacttcgaa cactacctgc aacagcatcc tgaactgccg cagaagttga ttgcgatgga
     1021 acctccattg cctgcagcat cgactgatgg catgcaagta acagagtttc actgtcatcc
     1081 ggcaaatacc tttgatgcga ttgcgcgcgt taaaacgatg ctgccaggca atatggaaag
     1141 ttacgagccg ttaagcgtga gtcagcgcag ccagctgcgc cgcattatgc tgtgcatctc
     1201 tgatacctcc gcgaagctgg cgaaactgcc aggcgtcagt aaagaagacc agaacctgct
     1261 gaaaaaactg cgcagcgata tgttaagcac cattgagtac gctccggtgt ggatcatcat
     1321 ggcggtagca ctggcgctcg gcattggcac catgattggc tggcgtcgtg tagcgatgac
     1381 catcggtgag aagattggta agcgcggcat gacgtatgcg caaggcatgg cggcacaaat
     1441 gacggcggca gtgtctatcg gtcttgccag ttatattggg atgcccgtct ccacaacaca
     1501 cgtcctctcg tctgcagttg cagggacgat ggtggtggac ggcggtgggt tacagcgtaa
     1561 aacggtaacc agcatcctga tggcgtgggt atttacttta ccggcggcaa tttttctttc
     1621 tggtgggctg tactggatag cattgcagtt gatttaacct tcctgaaaat gcccggtcct
     1681 ggcgatcggg catttccatt tttgactagt gataaccacg cgcggtcata aaatccgtaa
     1741 tcgctttttc tgcatcaacc aacacctgtt ccagtggctg attggcatcg atatcaacca
     1801 gttgtgcacc accaaaggtt aactgtggcg ttatggcaat cttcctcgcc agcgattccc
     1861 gtttatggtc gggtttacgt gcacaggcaa cttcaaggtc aacattgagt ttgatgacca
     1921 gatcaggctt atggctcgcc atccagtgaa acgctttacg ttcctggctt gccagccatg
     1981 agacaaaacg accaccttca acgttaggtg ggaacaccgt accatcgtaa gcgccaggaa
     2041 tttggtcctg aggataacgg tcggttagaa caattaaccc gcgacgacga caggcaagca
     2101 tatgacgaaa gcgcagtaag cgacgggcga caaacgctgt aattaccagc gccggaactg
     2161 gtccaggcaa tttttttgct gttttcacct gatttcgttc aattgtttta tgtaaggatt
     2221 ttcccatcaa cggtaatttt gtcactgcac gaccgacatt tccggcctgt tttcctaaat
     2281 gaactctttc ggcagcacca tatttttcga caacggtaat gagatgttca cacaccgttg
     2341 acttgcctga accatcgcta ccaataacgg caattaatgg aggtgttatt gactgcatga
     2401 atatatcctt aaatttaaat atccatccaa aatatttatt tggttaatat gtttttatga
     2461 aagcgtaatt caggtcaatg tcacaattaa ccattgtcac aataaggttg aacggatatt
     2521 ctgagcacgg accacgtaat attcaataca ttatttactg ccgggttttt cgtgaatcaa
     2581 cgtaatatgt caataattaa ttccacgcca gtgcgtgtta ttgccattgt aggatgtgat
     2641 ggttcaggta aatcgaccct cacggcaagc ctggtaaatg aactggcagc aagaatgcca
     2701 gcagaacaca tttatctcgg gcaatcgtcc gggcgaattg gcgaatggat ttcacagctc
     2761 cctgttattg gcgcaccttt tgggcgttat ttgcgaagta aagcggcaca tgtgcacgaa
     2821 aagccctcaa caccgcctgg caatattact gctctggtta tctatctgct ttcctgctgg
     2881 cgggcgtaca agtttcgcaa aatgttgtgt aaaagccagc aaggctatct gctcatcacc
     2941 gaccgctacc cgcaggttga agtgccgggt tttcgctttg atggcccgca attggcaaaa
     3001 accacgggcg gtaacggttg gataaaaatg ttaaggcagc gcgagctgaa gctgtaccaa
     3061 tggatggcat cttatttgcc cgtattgttg attcgtcttg gcattgatga acaaacggcg
     3121 tttgcgcgta aacctgacca ccaactggca gcgttacagg aaaaaatcgc cgttacgccg
     3181 caactgactt tcaacggcgc caggatcctt gaactggatg gtcggcaacc agccgatgaa
     3241 attctgcaag cgtcactacg cgcaattcac gccgcgcttt cctgatcacc aaaatggata
     3301 ggggatgatt taacgaatgg atgcactaca aacacaaact gttattagta caaccgcgcc
     3361 gcagcccagc tacattccgg ggctaattgc ggtggtcggg tgtgatggca ccggtaaatc
     3421 cacactgacc accgacctgg tgaaatcgct gcaacaacac tggcaaaccg agcggcgcta
     3481 tctggggctg ctctccggcg aagacggcga caaaatcaaa cgattgccgt tggttggcgt
     3541 ctggctggaa cggcgactgg cggccaaatc ctcgaaaacc caaagcatga aaaccaaatc
     3601 tccggcgcta tgggcggcgg tgattatgta ctgcttctcg ctgcgaagaa tggcgaatct
     3661 acgcaaggtt cagcgactgg cgcaaagtgg cgttctggtg gtcagcgatc gcttcccgca
     3721 ggctgaaatt tcgggctttt attatgacgg accggggatt ggcgtcgaac gtgcgaccgg
     3781 gaaaatcagc atgtttctgg cgcagcgcga acggcgttta taccaacaaa tggcgcaata
     3841 tcgcccggaa ttaattattc gcctgggcat tgatattgag actgccatct cccgcaagcc
     3901 tgaccatgac tatgccgagc tgcaggacaa aatcggtgtc atgtcgaaga ttggctataa
     3961 cggcacaaaa attcttgaga ttgattccag agcgccctac agcgaagtgc tggaacaggc
     4021 acagaaggcg gtttctctgg ttgccatcgt ttctgaccgc cgaagtttaa cttaaggctg
     4081 gcgggatact ttttactatc aacgataagg tcaggctgaa ttggcgggtt ttaacatcaa
     4141 acattggttt gcagatggcg cgtttcgcac cattattcgc aatagcgcct ggttaggctc
     4201 cagtaatgtc gtgagcgcct tgctggggct gttggcgctc tcgtgtgccg gtaaagggat
     4261 gacgcccgcc atgtttggcg tactggtgat tgtgcaatcg tacgccaagt cgatcagcga
     4321 ttttattaag tttcagacat ggcaactggt ggttcagtac ggaacaccag cattaaccaa
     4381 caataatccg cagcaattcc gcaatgtcgt ctcattttcc ttctcgctgg atatcgtcag
     4441 cggcgcggtg gcgattgtcg gtggcattgc cttactgcca ttcctttccc attcattagg
     4501 tctggatgac caaagttttt ggctggcagc gctctattgc acgctcattc cttcaatggc
     4561 ttcctccacg ccgaccggca ttctgcgtgc ggtagatcgc ttcgatttaa ttgctgtaca
     4621 gcaggcgacg aaaccttttc tgcgcgcagc ggggagcgtc gtagcctggt attttgactt
     4681 tggttttgcg ggttttgtta ttgcctggta cgtgtcgaat ctggttggcg gcaccatgta
     4741 ctggtggttt gccgcgcgcg aattacgccg ccgaaatatc cataatgcct tcaaattgaa
     4801 tctgtttgag tctgcccgac acatcaaagg cgcgtggagt tttgtctggt caaccaacat
     4861 tgcccactcc atctggtcgg cgcgtaactc gtgcagcaca gtgctggtgg ggatcgtgtt
     4921 aggacccgct gccgccgggt tatttaaaat cgccatgaca ttcttcgacg ccgccggaac
     4981 gccagcgggt ttgctgggta aaagttttta cccagaggtg atgcgtttag atccgcgcac
     5041 caccagaccg tggttgttag gtgtgaagtc cggtttactg gcgggcggaa tcggtattct
     5101 ggtggcgctc gcggtgttta tcgtcggcaa gccgctcatt tcgctggtgt ttggcgttaa
     5161 gtatctcgaa gcgtatgacc tgattcaggt gatgctgggc gcaattgtga tttcgatgct
     5221 gggcttccct caggagtcat tgctattgat ggcgggaaaa cagcgcgctt ttctcgtggc
     5281 gcaaaccatc gcctcaatcg gctacatcgt attgctgttc atgttctgtc atctgtttgg
     5341 tgtgctggga gcagcgtttg cttacttcgg tgggcaatgt ctggacgtgg cgctctcgtt
     5401 aattccaacc ttaaaggcat tttttcagcg ccattccttg ctttataacg cagccggaga
     5461 gaaatcctga tgatgagaaa atatttcccg cttgaggcga gcgagcgtct atttgttgcg
     5521 attgaagaag atgatgtcgt tgatgcacag gttagtttgc cgccgactat tgcactaagt
     5581 tgtaccaccg aaatcattca tgataattac gcgttatgcc tgcaattctg gctcaatggc
     5641 gttgatcgtc aggaattact gcgtttagtt cgcaaacaag ccaaaggcga tgagctgact
     5701 gctgatgagc gcaaacaatt taaatacatg cgagcgcgct acaagcacct gcgttttgcg
     5761 caacggctgt atttaaagaa gcatcaggcc ggatttttat tcggcaaaac gaccgttttt
     5821 cttgggcgtt ttcaggacgg ctttcgtaac ggtaagaaaa atattgtgtc gtattacggc
     5881 aacctgttac ggatttattt aagctctccg gtctggtcgt tggtgaacta ttcattacgc
     5941 catagccagt tagaaagcgt aagcagtttt attgcctatc ggcaaaagca aatgcatacc
     6001 ctgaaagaga ttattgccaa accgcgacta accggcaggg aatttcatga tgtgcgaaaa
     6061 atcatcagcc aacaggtttc ttattacgac accttaagat cgcttgatcc tgaaaataaa
     6121 gaggcgttac agatttcccg tttcctcgcc gccattaatg gcctgatggg agataaacac
     6181 gacgacatgg tggcggatga tatggaaaac cgccagtcgt atgatgcacc gttggcgctg
     6241 gatagcgata ttcgtcagcg cttagagctg ctgatttcgc gttttccgct ataaccggag
     6301 caatgatgtc atctcgtttg attattgcgc tgattatcat gcttttagcc ccgggtgtgc
     6361 aggcacacaa tttcgtcact ggcaaaacgg ttacccctgt ttatattcag gaaggcggcg
     6421 agctgcttct gaatagcgac gacgaaattc actaccaaaa gtggaacagc acacaattgg
     6481 cgggaaaagt ccgcattatc cagtatatcg ccgggcgtaa gtcggcgaag aaaaagaact
     6541 cactgttaat taaagcggtt gaagcggcaa actttccgca ggatcgtttt cagcccacca
     6601 ccattgtgaa caccgatgat gccatctttg gtactggcta ttttgttgtt ggcaaaatcg
     6661 aaaaaaataa acgccgctat ccgtgggcgc aattcgttat tgacggcaat ggacagggac
     6721 gcgttgcctg gcgcttgccg gagcagagtt caaccattct ggtgctgaat aaagccgggc
     6781 aaattcagtg ggcaaaggac ggcagcctga cgccggaaga ggtagatcat gtcattgcgt
     6841 tggcgcagaa actgatcaat gaataaaaaa ctcaaaggat tgagggcgtt agtgagatga
     6901 agctggttga acactacatc atgcgtggta cgcgccgtct ggtgctgatt atcgtcggtt
     6961 ttctgatctt tattttcgcc agctactccg cacagcgtta tctgaccgaa gcggcaaatg
     7021 ggacattagc tcttgatgtt gtgctggata tcgttttcta caaagtactg attgcactgg
     7081 agatgttgtt acctgttggt ctgtatgtgt cagttggcgt gacgctaggg cagatgtata
     7141 ccgactcgga aattaccgct atctctgcgg cggggggcag tccgggacgc ttgtacaaag
     7201 ccgttcttta tctggcgata ccgctaagta tttttgtcac ccttctgtcg atgtatggtc
     7261 gtccgtgggc ttatgcgcag atttatcaac tggagcagca gtcgcagtcg gagctggatg
     7321 ttcgccagtt gcgggcaaag aaatttaaca ctaacgataa cggacgaatg atcctttcgc
     7381 agacggttga tcaggataat aatcgcctga ctgacgcgct gatttatact tctactgcca
     7441 atcgaacccg tattttccgc gcccgttcgg ttgatgtggt tgacccatca cccgagaaac
     7501 cgaccgttat gttgcacaac ggcactgcct atcttctcga tcatcagggg cgtgacgaca
     7561 acgaacagat ctaccgtaat ctgcaattac atctgaatcc gctggatcaa agccctaacg
     7621 tcaaacgcaa agcaaaatcg gtcacggagc tggcgcgctc cgcctttcct gccgatcatg
     7681 ccgaactgca atggcgacaa agccgtggcc tgaccgcatt gttgatggcg ctgctggcca
     7741 tttcattaag tcgggtaaaa ccgcggcaag ggcgattttc aacgttattg ccactgacgt
     7801 tgctgtttat tgccattttt tatggcggcg acgtctgccg tacgctggtg gctaacggcg
     7861 cgattcctct cattcctggt ttgtggttag tgcccggact catgctaatg ggcctgctga
     7921 tgctggtcgc acgcgacttc tctttgctgc agaaattttc ccgatgaatg ttttcagtcg
     7981 ctatttaatc cgtcatctct ttctcggttt tgccgccgcc gcagggctat tgctgccgct
     8041 ttttaccacc ttcaacctga ttaacgaact ggatgatgtc agcccgggcg gttatcgctg
     8101 gactcaggcg gtgctggtgg tgctaatgac cttaccgcgc acactggtcg aactttcgcc
     8161 gtttatcgcc ttattgggag ggattgttgg cctggggcag ttatcgaaaa acagtgagct
     8221 taccgccatt cgcagcacgg ggttttctat cttccgtatt gcactggtgg cgctggttgc
     8281 cgggatattg tggactgttt cgttaggcgc gatagatgag tgggtggcgt cgccattgca
     8341 gcaacaggcg ctgcaaatca aatcgaccgc caccgcgttg ggggaggacg atgacattac
     8401 cggcaatatg ctgtgggcca ggcgcggcaa tgaatttgtg acggtgaaat cgctgaatga
     8461 acagggccag cctgtgggcg tggagatttt tcattatcgt gacgatcttt cgctcgaatc
     8521 ctacatttat gcacgcagtg ccaccattaa agacgacaaa acgtggatcc tgcatggtgt
     8581 gaatcataaa aaatggctta acggtaaaga aacgctggaa acatcagata atcttgcctg
     8641 gcaatcggcc ttcaccagta tgaatcttga agagttatca gtgccgggga atactttttc
     8701 tgtccgtcag cttaatcatt acatccatta tttgcaggaa accgggcaac ccagcagcga
     8761 ataccgcctt gcactgtggg aaaaactggg gcaacccatc ctgaccctgg cgatgatttt
     8821 gctggctgtg ccgtttacct ttactgcccc gcgttcgcct gggatgggta gccgtctcgc
     8881 tgtaggcgtc atcgtgggct tactcacctg gatcagctat caaatcatgg ttaatctggg
     8941 attgctattt gcgttaagcg cacctgttac cgcgctcggt ttaccggtag cgtttgtgtt
     9001 ggtggcgttg agcctggtgt attggtatga cagacaacat taaacccgcc gtggcgcaaa
     9061 caccacggcg ggctcacatt actgcttcag agtagcaaat gcctgaatga tggcatcgat
     9121 ttgctcacga ctgtgggcag catttacgct acagcgcagc agcgtgatgc ctgcaggcgc
     9181 ggcaggcggc aggataagat tcacatacac gcccagcgag ataagctcgc gccagatgcg
     9241 taaaccttcc tctttcgaac cgattattac tggcactacc gggctaatat gtgaacttaa
     9301 ctcataaccc agtttttgca gaccgtcata cagatggttg gcgttatcca tcagcttttg
     9361 ccgcagctct ggttgtgtgg caatagttct taacgacgag cgcacggtcg caatgcagga
     9421 cggagaagga gaggcggtaa aaatatacgg gcggctgccg tagcgcagca cttccatcgc
     9481 ttccgaaccg acagcaaaac cgccaatcga ggccagactt ttactgaagg tgccaacgat
     9541 aatatccaca tcatcttcaa cgccgacagc ttcagccagt ccgcgccccg ttgcgcctaa
     9601 cacgccaaac gaatgcgctt catcgacaat caaataacca ccgagacgac gtttgatatc
     9661 aacaatttcc gccagcggcg caacgtcacc gagcatgctg tagatgccct caacgatgat
     9721 aatcgcctct ttcgcccgtt caccgaggcg aaccatgcgc cgttccagat ctttagcatc
     9781 gttatggcgg aaacgaataa tttccgcgcc accgagagaa caggcgtcat aaatactggc
     9841 gtggctgtcg gcatccagta acaccacggc gttatggtcg gcaagtgcgc tgatgacgcc
     9901 taaattagcg gtataaccgg ttgaaaaaac gatagcggta gggcgcttga aaaactcggc
     9961 aatctctttc tctaacgcca ggtgcggcgc ataactgccg tttgccatac gagaaccggt
    10021 ggtgccggtg ccttgggccg ctaacgctgc ctgaccttcg gcgatggcat catgattaaa
    10081 ggttaaaccg aggtaattat tggtcccggc cagaatcact tttttattgc cgatccggcc
    10141 ttcggtggcg gaatacactt catcaataca ggtgccaaac ggtgttaacc cgttgtcact
    10201 aaattgcagt cgctcgccag caaggcgcgc atatttatcg tatagcccca ttatttattc
    10261 tccagccatg gaagtagagt ttccattaat tgcgcagggg ttttgacatc cagcagaata
    10321 ttaataggaa tggaaatatc gaatctgtct tccagcatca ttaagagatc catcactttg
    10381 atcgactcca ggccaagatc gttgacgaga tcgctgtcgg gcttaatttc cacaccgttt
    10441 tcgaccaaat cctgtaaaca ggaaaggatg taatccatta ctatttcacg atttaccata
    10501 gaaacgttaa cacaccttta aacaagacaa tcaaaatagc ccgttgcgta atgcttgttc
    10561 caggctaatc ccgggaaacc aattaatatc ttcagaaata cggttattac ttgccgacca
    10621 gtcggcgtgg gttaattcac gaattttcga gcgggtcagc ataggttctt tacctgccag
    10681 gcgactcaac gcggtactga tatccgcaag gcaggtgagt accggcagag gaataccaac
    10741 cattcgcacg gaaccacaac gggcgtcggc ggcaagctgt cgtacgtgtt gccagtcata
    10801 gccgccgggg acgccatcgc ataattcata ggtttgcgtc tgtacagttt cggcgcttaa
    10861 ccactgaccc actgcttgcg cgaaatcggt gacgtgcagg aaagagagct gtgtatctgg
    10921 cgcaccaagt cgtggcagca ggccgcgcag catccagtca aacagcggtt ttaactcttt
    10981 atcgccggga ccatacacgg ctgtcgggcg aaaaacgccc agcgtaattt cgtcagccat
    11041 tgcagtcaga cgttgttcgg cgacgtgttt ggaatttgcg taccaggaga gctcgggatg
    11101 gcgcgccgcc agcgaagaga taaacagaaa acgttggcaa aagccgctct cttttgctgc
    11161 ttgcatcagg cgcaggctgc cgtcaacgtt acagtgggta aaaatctctt ctttgtgtcc
    11221 gcgcacttgc ccggcgcaat ggaccaccgc gctggctccg gcaaccagtt cgctaagcga
    11281 atgtgtatct tccagcgaac cgcgcaccca agtaagatta tcattgacgt gagcgcgggc
    11341 agtacgcgtc aatgcgcgaa cgtgaaagcc gcgggcgagc aggttatcga taatatattt
    11401 accgataaac ccggtagcgc ccgtcaccgc gacagtttga ttcatgccag ggattcctgc
    11461 acatgaaggc ttgcagcata agccttctga taacgttttt tcgcttccgc acgggcaggc
    11521 ttgccggagg acgttcgggg aatgctgtgg ggcggcaaca gatcgatagc cgcggttacg
    11581 ccaaattcgc tttggatccg tgctgccagc gcgtggataa gctgtccgcg acgttcttca
    11641 tcgctgatcc gacactggat ctgcaaaatg attttttcct gggcggtaac aaaagcaatc
    11701 gcatcgccag aatgaatttc cggttcctgt tccgctatat attcaatatc ctgcggccag
    11761 atattacggc cacgaataat aatcagatct ttaatgcgtc cggtgacata cagataaccg
    11821 tccagcagat aaccgaggtc gccggtgtct aaccagcccg tcgcggcaat ctcgtcttgc
    11881 gaagcctggt cgccaaagta accgctcatc agactggggc cggagatgca aatatggcct
    11941 accacacgtt ccgcgaccgg catacctgct tcattgcgga tttcaatacc atgttccggc
    12001 aacgctttgc cgcagttgac gaaagtcgat acggcgcgtg tctctgcacc cggcgcgacg
    12061 gctttaccct gatattcgag gatgtcgcga tccacttcgt taaccacaac cccggaggct
    12121 tcatcagaga agctgacagc cagcgcattt tctgccagtc cgtagcacgg catgaaagtt
    12181 ttattgtcaa agttaacctg acggaaacat tcagcgaatt gatggagttg ttctgcggag
    12241 atgggttctg caccaatacc agcgacgcgc cagcaggaaa gatccagttc agcgagatct
    12301 ttttcattca cgcggcgctg gcacaattca tagccaaacg gcggcgcaac ggaaacggtg
    12361 ccgcgatttt tactgatcaa tttaagccat tgcagaggac gcatggcaaa atcctgagtg
    12421 cgcaaataat ctactgaaag ctgcgtggag acgggggtca ggagaaagcc gaccagtccc
    12481 atatcatggt agaaaggcag ccaggagacg cagcggtcgc cagggcgtaa tttaatgccg
    12541 tcgtggctta tagcacgtag attagccatc acttcgcgat gggtgataat gacgccacgg
    12601 ggaaaacggg tgctgccgga ggtgtactgg aggtaggcga tatcgttcgg aactggacgc
    12661 tggagcgcaa catcggcttc cggtaatgcc ttaaaccagg cgtggcttaa aacatgtaat
    12721 tcggggttgt tatgcgtcgc ggcattgacc agtggcaacc actcatcacc agtgataatg
    12781 gctgcgggct ggcaacttgc cagtaaaccc tgcaatttgg cgctccagga atcccgctga
    12841 ccaacgccca ttggaatcgc caacgggacg gcgactaagc cggcatactg gcaggcaaaa
    12901 aaagcctcta cgaacccgct acttgtttcg gcaatcagtg ccacgcgatc gccttttttc
    12961 aggttcagcg ataacaaccg cttcgcacca gcttcggcac gtgcttttaa cgtctgatat
    13021 tccagttgat cttcaagttg gcaacgtctg tcataaaaat tcattccggc gctactcaga
    13081 gcggcgtagt ccaaagcatc aaccagcgtt ggaaaatcgg catagcgcat aggtagggaa
    13141 tgcgtaaaga ttttattaga catatacacc acgaaaaaat tggatatcca aaataaaatg
    13201 acaataatgc ggtggattat ataggcataa aagataaatg agatagtatt ttcatcggtg
    13261 tgaaaatgaa cattgattta aataatgagt ttaag
//
LOCUS       DAZFQD010000142        13240 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.142, whole genome shotgun sequence.
ACCESSION   DAZFQD010000142 DAZFQD010000000
VERSION     DAZFQD010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13240
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.142"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(53..214)
                     /gene="rsmS"
                     /locus_tag="ACHKTW_004189"
     CDS             complement(53..214)
                     /gene="rsmS"
                     /locus_tag="ACHKTW_004189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HFO6465109.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPARTVLRALDIVKRDYEKK
                     LTRDDEAEK"
     gene            complement(228..755)
                     /gene="priC"
                     /locus_tag="ACHKTW_004190"
     CDS             complement(228..755)
                     /gene="priC"
                     /locus_tag="ACHKTW_004190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HFO6465110.1"
                     /translation="MKTALLLEKVEGQLATLRQRCAPVAQFATLSARFDRHLFQTRAT
                     TLQACLDEAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREASAWSLREWDSAP
                     PKIARWQRKRIQHQDFERRLREMVAERRARLTRVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            825..1202
                     /locus_tag="ACHKTW_004191"
     CDS             825..1202
                     /locus_tag="ACHKTW_004191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HFO6465111.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            1355..1906
                     /gene="apt"
                     /locus_tag="ACHKTW_004192"
     CDS             1355..1906
                     /gene="apt"
                     /locus_tag="ACHKTW_004192"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFO6465112.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISETYDL
                     EYGTDQLEFHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVTFPGH"
     gene            2035..3966
                     /gene="dnaX"
                     /locus_tag="ACHKTW_004193"
     CDS             2035..3966
                     /gene="dnaX"
                     /locus_tag="ACHKTW_004193"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFO6465113.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEANGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTQVPPQ
                     PQSAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERL
                     ASVTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPE
                     LAAKLAAEAIERDAWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNR
                     GAQQKLAEALSTLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNI
                     QTLRRFFDAELDEESIRPI"
     misc_feature    3299..3363
                     /gene="dnaX"
                     /locus_tag="ACHKTW_004193"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            4019..4348
                     /locus_tag="ACHKTW_004194"
     CDS             4019..4348
                     /locus_tag="ACHKTW_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFO6465114.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            4348..4953
                     /gene="recR"
                     /locus_tag="ACHKTW_004195"
     CDS             4348..4953
                     /gene="recR"
                     /locus_tag="ACHKTW_004195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFO6465115.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            5063..6937
                     /gene="htpG"
                     /locus_tag="ACHKTW_004196"
     CDS             5063..6937
                     /gene="htpG"
                     /locus_tag="ACHKTW_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HFO6465116.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWEKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTHTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            7118..7762
                     /gene="adk"
                     /locus_tag="ACHKTW_004197"
     CDS             7118..7762
                     /gene="adk"
                     /locus_tag="ACHKTW_004197"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004017 - adenylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFO6465117.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKPVAEVRADLEKILG"
     gene            7998..8960
                     /gene="hemH"
                     /locus_tag="ACHKTW_004198"
     CDS             7998..8960
                     /gene="hemH"
                     /locus_tag="ACHKTW_004198"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HFO6465118.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEDGSPLMVYSRQQQQALAQRLPETPVALGMSY
                     GSPSLESAVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMAPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(8957..9916)
                     /gene="aes"
                     /locus_tag="ACHKTW_004199"
     CDS             complement(8957..9916)
                     /gene="aes"
                     /locus_tag="ACHKTW_004199"
                     /EC_number="3.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
                     /protein_id="HFO6465119.1"
                     /translation="MKPENKLPVLDLISAEMKTVVNTLQSDLPSWPATGTIAEQRQYY
                     TLERRFWNAGAPEMATRAYMVPTKYGQVETRLFCPQPDSPATLFYLHGGGFILGNLDT
                     HDRIMRLLASYSQCTVIGIDYPLSPEARFPQAIEEIVAACCYFHQQAEDYQINMSRIG
                     FAGDSAGAMLALASALWLRDKQIDCGKIAGVLLWYGLYGLRDSVTRRLLGGVWDGLTQ
                     QDLQMYEEAYLSNDADRESPYYCLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLA
                     AHQQPCEFKLYPGTLHAFLHYSRMMKTADEALRDGAQFFTAQL"
     gene            10068..11372
                     /gene="gsk"
                     /locus_tag="ACHKTW_004200"
     CDS             10068..11372
                     /gene="gsk"
                     /locus_tag="ACHKTW_004200"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HFO6465120.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEALYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVDLVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSLEESYWDR"
     gene            complement(11505..13181)
                     /gene="ybaL"
                     /locus_tag="ACHKTW_004201"
     CDS             complement(11505..13181)
                     /gene="ybaL"
                     /locus_tag="ACHKTW_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaL family putative K(+) efflux transporter"
                     /protein_id="HFO6465121.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKSIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSLAAFFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARVKNPD
                     IEIIARAHYDDEVAYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
BASE COUNT         3214 a         3585 c         3519 g         2922 t
ORIGIN      
        1 gttttgcgtt tgcccacaat cttaggggac atttagcggc cccatttatt tctcactttt
       61 ccgcctcatc atcgcgcgtt aatttctttt cataatcgcg ttttacaata tccagcgcgc
      121 gcagcacggt gcgggctggg atctgatttt cttccagcag cacaatcaaa tcgacagcca
      181 gtttgacatc gtcaggggca ttttccagtg acatactctc tccattgcta gcgggttaag
      241 cgcgctagcc tgttttcgat tttttccagc gcatggcggc agcgtgccag gcgcgcttca
      301 taggcttcca cttcacgatg cagcgtttgc tgttccacga gatcggtcac ccgcgtcagg
      361 cgggctctgc gttcggcaac catctcacgt agccgccgct caaaatcctg atgctgaata
      421 cgtttacgct gccagcgggc aattttcggt ggcgcactgt cccactcgcg caatgaccag
      481 gcggaggctt cacgcgcgat ggcttccagt tgtgccgcca gatgttccgc cagccaggcc
      541 acttgcggca gctgttgctg ctcaactgca tgacgaagcg cagccagatt atcgcccgcc
      601 tcatcgagac aagcctgcag tgttgtcgca cgggtttgaa aaaggtgtct gtcaaaacgg
      661 gcacttagcg tggcaaattg cgctaccggg gcacaacgct gacgcagcgt agcgagctgt
      721 ccttccactt tttccagcag cagggcggtt ttcacgataa gacctcaatg aaaatgataa
      781 ttgttatgct aaagtagcca ctctgtaagc tgacaacact caatatgcaa cgaatcattt
      841 taatcatcat tggctggctg gcggtagtgc tgggtacgct gggcgtggta ttaccggtat
      901 taccgacgac gccgtttatc ctgctggcgg cctggtgctt tgcccgttct tccccgcgct
      961 ttcacgcttg gttgctgtac cgctcatggt ttggcagcta tctacgtttc tggcagaaac
     1021 atcatgcgat gccgcgcggc gtcaaaccgc gggcgatttt gcttattttg ctcacgtttg
     1081 ccatttctct gtggttcgtc cagatgccat gggtgcgcat catgttgctg gtaattctcg
     1141 cctgtttgct tttctatatg tggcgaattc cggtgattga tgaaaagcaa gaaaagcact
     1201 gaagcacaac aatcgcagtt gcaattattg cgtacagcca gtacattctg gcgttttcga
     1261 gcacaggcgc aggcggtcaa aggttaaaca actgttactt ttgatacgtt taaaacgcgc
     1321 cgtgagtacc accgtaacaa gcaggcatac acttatgacc gcgactgcac agcagcttga
     1381 gtatctcaaa aatagcatca aaagcattca ggactaccca aaacccggca ttcttttccg
     1441 cgatgtcacc agcttactgg aagacccgaa agcttacgct ctcagcatcg acttgctggt
     1501 tgagcgttac aaaaatgcgg gcattaccaa agttgtcggc accgaagcgc gtggcttctt
     1561 gtttggcgct ccggtagctc tgggtctggg cgttggcttt gtaccggtcc gtaaaccggg
     1621 caaactgccg cgtgaaacca tcagtgaaac ttacgacctg gaatacggca ccgatcagct
     1681 ggagttccac gttgatgcca tcaaaccggg cgacaaagtt ctggtggtgg acgatctgct
     1741 ggcaaccggc ggcactatcg aagcgaccgt taaactgatc cgtcgtctgg gtggtgaagt
     1801 ggctgacgct gcgttcatta tcaacctgtt cgatctcggc ggcgaacagc gtctcgaaaa
     1861 acagggcatt accagctaca gccttgtcac gttcccgggc cattaattat cgccagtctt
     1921 gtgctgccca cgctacggac agcacaagat gtgcattcag cctcgccgtt ctgacggggc
     1981 tgtgttagca ttaccccttc gtgaatccac cttccagcgt ttcagagcct gccaatgagt
     2041 tatcaggtct tagcccgaaa atggcgccca caaacctttg ctgacgtcgt cggccaggaa
     2101 catgtgctga ccgcactggc gaacggcttg tcgttagggc gtattcatca tgcttatctt
     2161 ttttccggta cccgtggcgt cggaaaaacc tctatcgccc gactgctggc gaaggggcta
     2221 aactgcgaaa ccggcattac cgcgacgccg tgcggcgtgt gcgataactg tcgtgaaatc
     2281 gagcaggggc gctttgtcga tctgattgaa atcgacgccg cctcgcgcac caaagttgaa
     2341 gatacccgcg acctgctgga taacgtccag tacgctccgg cgcgtggtcg tttcaaagtt
     2401 tatctgatcg acgaagtgca tatgctgtcg cgccacagct ttaacgcact gttaaaaacc
     2461 cttgaagagc cgccggagca cgttaagttt ctgctggcga cgaccgatcc acagaaattg
     2521 ccggtgacga ttttgtcgcg ctgtttgcaa tttcatctca aggcgctgga tgtcgagcaa
     2581 attcgccatc aacttgagca catcctcaac gaagaacata tcgctcacga gccgcgggcg
     2641 ttgcaattgc tggcgcgcgc cgctgaaggc agcctgcgag atgccttaag tctgaccgac
     2701 caggcgattg ccagcggtga cggccaggtt tcaacccagg cagtcagtgc gatgctgggt
     2761 acgcttgacg acgatcaggc gctgtcgctg gttgaagcga tggtcgaggc caacggcgag
     2821 cgcgtaatgg cgctgattaa tgaagccgct gcccgtggta tcgagtggga agcgttgctg
     2881 gtggaaatgc tcggcctgtt gcatcgtatt gcgatggtac aactttcgcc tgctgcactt
     2941 ggcaacgaca tggccgccat tgagctgcgg atgcgtgaac tggcgcgcac cataccgccg
     3001 acggatattc agctttacta tcagacgctg ttgattggtc gcaaagaatt accgtatgcg
     3061 ccggaccgcc gcatgggcgt tgagatgacg ctgctgcgcg cgctggcgtt ccatccacgt
     3121 atgcctctgc ctgagccaga agtgccacgc cagtcctttg cgcccgtcgc gccaacggca
     3181 gtaatgacgc caacccaggt gccgccgcaa ccgcaatcag cgccgcagca ggcaccgact
     3241 gtaccgctcc cggaaaccac cagccaggtg ctggcggcgc gccagcagtt gcagcgcgtg
     3301 cagggagcaa ccaaagcaaa aaagagtgaa ccggcagccg ctacccgcgc gcggccggtg
     3361 aataacgctg cgctggaaag actggcttcg gtcaccgatc gcgttcaggc gcgtccggtg
     3421 ccatcggcgc tggaaaaagc gccagccaaa aaagaagcgt atcgctggaa ggctaccact
     3481 ccagtgatgc agcaaaaaga agtggtcgcc acgccgaagg cgctgaaaaa agcgctggaa
     3541 catgaaaaaa cgccggaact ggcggcgaag ctggcggcag aagccattga gcgcgacgcg
     3601 tgggcggcac aggtgagtca actttcgcta ccaaaactgg tcgaacaggt ggccttaaat
     3661 gcctggaaag aggagagcga caacgcagta tgtctgcatt tgcgctcctc tcagcggcat
     3721 ttgaacaacc gcggtgcaca gcaaaaactg gctgaagcgt tgagcacgtt aaaaggttca
     3781 acggttgaac tgactatcgt tgaagatgat aatcccgcgg tgcgtacgcc gctggagtgg
     3841 cgtcaggcga tatacgaaga aaaacttgcg caggcgcgcg agtccattat tgcggataat
     3901 aatattcaga ccctgcgtcg gttcttcgat gcggagctgg atgaagaaag tatccgcccc
     3961 atttgatcgt aagcacagct tacgttcgtc atccttaacg tgattgagag agaaacctat
     4021 gtttggtaaa ggcggtctgg gtaacctgat gaagcaagcc cagcagatgc aagaaaaaat
     4081 gcagaaaatg caggaagaga tcgcgcagct ggaagttacc ggcgaatctg gcgcaggtct
     4141 ggtaaaagtg accatcaacg gtgcacacaa ctgccgtcgc gtagagatcg acccgagcct
     4201 gctggaagac gacaaagaga tgctggaaga cctggtggct gcagcattca acgacgcagc
     4261 acgtcgtatt gaagaaacgc agaaagaaaa aatggcctct gtatcctccg gaatgcagct
     4321 gccgcctggc tttaagatgc cgttctgatg caaaccagcc cgctgttaac acagcttatg
     4381 gaagcactgc gctgtctgcc gggcgttggc ccgaagtcgg cgcagcgtat ggcgttcacg
     4441 ctgcttcagc gcgatcgtag cggcgggatg cgtctggcgc aggcgctcac ccgggcgatg
     4501 tcggaaatcg gccactgcgc cgattgccgc actttcaccg aacaggaagt ctgtaacatc
     4561 tgttcgaatc cgcgtcgtca ggaaaacggt caaatctgtg tggtggagag tccggcggac
     4621 atctacgcca ttgagcagac ggggcagttt tcaggccgtt attttgtgtt gatggggcac
     4681 ctgtcaccgc tggacggcat cggtccggat gatatcggcc ttgatcgtct ggaacagcgt
     4741 ctggcagagg aaaaaatcac tgaagtgatc ctcgccacca atcccacggt tgaaggtgaa
     4801 gctaccgcta actacattgc cgagctttgc gcgcaatatg acgtggaagc cagccgaatc
     4861 gctcatggcg taccggttgg cggcgaactg gaaatggtcg atggcaccac gctgtcacac
     4921 tcccttgccg ggcgtcataa gattcgtttt taagcaaacg agagcaggat cacctgctct
     4981 cgcttgaaat tattctccct tgtccccatc tctccctcat cctgttttta accttaaaat
     5041 ggcattattg aggtagacct acatgaaagg acaagaaact cgtggttttc agtcagaagt
     5101 gaaacagctt ctgcacctga tgatccattc tctctattcc aataaagaaa tcttcctgcg
     5161 tgagcttatc tctaacgcct ccgatgcggc ggacaagctg cgtttccgtg cgctatctaa
     5221 cccggacctg tacgaaggtg atggcgaact gcgcgttcgt gtctctttcg ataaagacaa
     5281 gcgcacgctg accatctccg ataacggcgt ggggatgacc cgcgacgaag tgattgacca
     5341 tctggggact atcgctaaat ccggtaccaa atcattcctc gaatccctgg gttctgacca
     5401 ggcgaaagac agccagctga tcggtcagtt tggtgttggt ttctactctg cgtttatcgt
     5461 ggccgacaaa gtgaccgtgc gtactcgcgc ggcaggcgaa aaaccagaaa acggcgtctt
     5521 ctgggaatcg gctggcgaag gtgaatatac cgttgccgac atcaccaaag aagatcgtgg
     5581 tactgaaatc accctgcatc tgcgtgaagg cgaagacgag ttcctcgatg actggcgcgt
     5641 gcgttccatc atcagcaaat actccgacca tatcgcgctg ccggtagaga tcgaaaaacg
     5701 cgaagagaaa gacggcgaaa ccgttatctc ctgggagaaa atcaacaaag cgcaggcgct
     5761 gtggactcgt aacaagtcgg aaatcaccga tgaagagtac aaagagttct acaaacacat
     5821 cgcccacgac tttaatgatc cgctgacctg gagccacaac cgcgttgaag gtaagcagga
     5881 gtacaccagc ctgctgtaca tcccgtccca ggctccgtgg gatatgtgga accgtgatca
     5941 taaacacggc ctgaaactgt acgtccagcg tgtgttcatc atggacgacg cagaacagtt
     6001 catgccgaac tatctgcgct tcgtgcgtgg tctgattgac tccagcgatc tgccgctgaa
     6061 cgtttcccgt gaaatcctcc aggacagcac ggtaacgcgt aacctgcgca atgcgctgac
     6121 caagcgtgtg ctgcaaatgc tggaaaaact ggcgaaagac gacgcggaaa aataccagac
     6181 cttctggcaa cagtttggcc tggtactgaa agaaggtccg gcggaagatt tcgcgaacca
     6241 ggaagcgatc gccaaactgc tgcgttttgc ttctacccat accgattctt ctgcgcagac
     6301 cgtatctctg gaagactacg tttcccgcat gaaagaaggg caggagaaaa tctactacat
     6361 caccgcagac agctatgcgg cagcgaagag cagcccgcac ctggaactgc tgcgtaagaa
     6421 aggcatcgaa gttctgctgc tttccgaccg catcgatgag tggatgatga actatctgac
     6481 tgagttcgac ggtaaaccgt tccagtcggt gtctaaagtt gacgagtcgc tggaaaaact
     6541 ggctgacgaa gttgatgaga gtgcgaaaga agcggagaaa gcactgactc cgttcatcga
     6601 ccgtgtgaaa gccctgctcg gcgagcgcgt gaaagatgtc cgtctgactc accgtctgac
     6661 cgatacgcca gcgatcgttt cgaccgacgc ggacgaaatg agcactcaga tggctaaact
     6721 gttcgctgcg gcgggccaga aagtgccaga agtgaaatac atcttcgaac tgaacccgga
     6781 tcacgtactg gtgaaacgtg cggcagatac tgaagatgaa gcgaagttca gcgagtgggt
     6841 agaactgctg ctggatcagg cgctgctggc agaacgcggc acgctggaag atccgaacct
     6901 gtttattcgt cgtatgaacc agctgctggt ttcctgatgt catgccggat gacctttgtg
     6961 tcatccggca tttttctttt catcatctgc actttccgca aattatctcg ccattaaccg
     7021 tttcagcctc aggtgccttt cttgaggcaa tcgcctgttg gtggtatcgt ttatcgcttt
     7081 ttcaaaaaat tcgacacatt ttaaggggat tttcgcaatg cgtatcattc tgcttggcgc
     7141 tccgggcgcg gggaaaggga ctcaggctca gttcatcatg gagaaatatg gtattccgca
     7201 aatctccact ggcgatatgc tgcgtgctgc ggtcaaatct ggctccgagc tgggtaaaca
     7261 agcaaaagac attatggatg ctggcaaact ggtcaccgac gaactggtga tcgcgctggt
     7321 taaagagcgc attgctcagg aagactgccg taatggtttc ctgttggacg gcttcccgcg
     7381 taccattccg caggcagacg cgatgaaaga agcgggcatc aatgttgatt acgttctgga
     7441 attcgacgta ccggacgaac tgattgttga tcgtatcgta ggccgccgcg ttcatgcgcc
     7501 gtctggtcgt gtttatcacg ttaaattcaa tccgccgaaa gtagaaggca aagacgacgt
     7561 taccggtgaa gaactgacta cccgtaaaga cgatcaggaa gaaaccgtgc gtaaacgtct
     7621 ggttgaatac catcagatga ctgcaccgct gatcggctac tactccaaag aagcggaagc
     7681 gggtaacacc aaatacgcga aagttgacgg caccaagccg gttgctgaag ttcgcgctga
     7741 tctggaaaaa atcctcggct aattcgaaag cgcgcacgga cagtcccctc gccccctcgg
     7801 ggagagggtt agggtgaggg gaacaggccc gcacaagcaa acttatcagc aatctcaggc
     7861 cggatattca ttcggccttt tacaaaaacc tcaatctgcg ctattccttt ttctgatttg
     7921 acctctcaca gcaattagtt cttcttcctc acttttccgc tacaattatc aacaagttga
     7981 atcgataaga ggcggtaatg cgtcagacta aaaccggtat cctgctggca aacctgggta
     8041 cgcccgatgc ccccacacct gaagcggtaa aacgctatct gaaacaattt ttaagcgaca
     8101 gacgcgtggt tgatacctca cggttgttat ggtggccgtt gctgcgcggc gtgattttgc
     8161 cgctgcgctc gccgcgtgtg gcgaagctgt acgcctcggt ctggatggaa gacggctcgc
     8221 cgctgatggt ttacagccgc cagcaacagc aggcgctggc acaacgttta ccggagacgc
     8281 ccgtagcgct gggcatgagc tacggctcgc catcactgga aagcgccgtc gatgaactcc
     8341 tggcagagca tgtagatcat attgtggtgc tgccgcttta tccgcaattc tcctgttcta
     8401 cggtcggtgc ggtatgggat gaactggcac gcattctggc gcgcaaacgt agcattccgg
     8461 ggatatcgtt tattcgtgat tacgccgata accacgatta cattaatgca ctggcgaaca
     8521 gcgtacgcgc ttcttttgcc aaacatggcg aaccggatct gctgctgctc tcttatcatg
     8581 gcattcccca gcgttatgca gatgaaggcg atgattaccc gcaacgttgc cgcacaacga
     8641 ctcgcgaact ggcttccgca ctggggatgg caccggaaaa agtgatgatg acctttcagt
     8701 cgcgctttgg ccgggaaccc tggctgatgc cttataccga cgaaacgctg aaaatgctcg
     8761 gagaaaaagg cgtaggtcat atacaggtga tgtgcccggg ctttgctgcg gattgtctgg
     8821 agacgctgga agagattgcc gagcaaaacc gtgaggtctt cctcggtgcc ggcgggaaaa
     8881 aatatgaata tattccagcg cttaatgcca cgccggaaca tatcgaaatg atggctaatc
     8941 ttgttgccgc gtatcgctaa agctgagcgg taaagaactg agcgccgtcg cgaagagcct
     9001 cgtcggcggt tttcatcatt cgtgaataat gcaaaaaggc atgcagcgta cctgggtaga
     9061 gtttgaactc acagggctgc tgatgcgccg ctaacgtctg gtaaagcaga cggctgtcat
     9121 ccagcagcgg atcgaactcc gccccggcaa taaaacaggg cggaacttcg cgagtgagat
     9181 cattattaaa cagacagtaa tacggtgact cgcggtccgc gtcgttgctt aaatacgcct
     9241 cttcatacat ctgcaaatcc tgttgcgtta agccatccca gacaccgccc aacagacgac
     9301 gagtcacgga atcccgtaat ccgtaaagcc cataccacag caaaacaccc gcaattttac
     9361 cgcaatcgat ctgtttatca cgcaaccaca acgcactggc gagcgccagc atggcacctg
     9421 cggaatcacc ggcaaagcca atgcgggaca tattgatttg ataatcctcc gcctgctggt
     9481 ggaaataaca acaagcagcc acaatttcct ctatcgcttg cggaaaacgc gcttcaggtg
     9541 aaagggggta atcaatacca atcaccgtac attggctgta gcttgccagc aggcgcatga
     9601 tgcgatcgtg ggtatcgaga ttgccgagaa taaaaccgcc tccatgcaaa taaaatagcg
     9661 tcgccgggct atctggctgc ggacaaaaga gacgtgtttc cacctgccca tattttgttg
     9721 gaaccatgta agctctggtt gccatttctg gagcgcccgc attccagaat cggcgctcaa
     9781 gcgtgtaata ctgtcgttgc tcagcaatcg ttcccgttgc gggccaggac ggtaaatccg
     9841 actgaagagt attcacaacg gtcttcattt cagcagaaat aaggtccaga acaggtagtt
     9901 tgttttccgg cttcatacaa aaactccttt caaattacgt cattgtaagg aaccactgcc
     9961 atgaaaatgc gatcccgcct gctgatattg aaactggctg cgtctcgcgc gctcccgtca
    10021 gattgtgtta acattcgccg ctcagttaac cacccgtaaa aacaaccatg aaatttcccg
    10081 gtaaacgtaa atccaaacat tacttccctg taaatgcacg cgatccgctg cttcagcaat
    10141 tccagccaga aaacgaaacc agcgccgcct gggtcgtagg tattgaccag acgctggtcg
    10201 atattgaagc gaaagtggat gatgaattca ttgagcgtta tggattaagc gccgggcatt
    10261 cactggtgat tgaggatgac gtagccgaag cgctttatca ggagctaaaa cagaaaaacc
    10321 tgattaccca tcagtttgcg ggtggcacca ttggtaacac catgcacaac tactcggtgc
    10381 tcgcggacga ccgttcggtg ctgctgggcg tcatgtgcag caatattgaa attggtagtt
    10441 atgcctatcg ttacctgtgt aacacttcca gccgtaccga tcttaactat ctacaaggcg
    10501 tggatggccc gattggtcgt tgctttacgc tgattggcga gtccggggaa cgtacctttg
    10561 ctatcagtcc aggccacatg aaccagctgc gggctgaaag cattccggaa gatgtgattg
    10621 ccggagcctc ggcactggtt ctcacctcat atctggtgcg ttgcaagccg ggtgaaccca
    10681 tgccggaagc aaccatgaaa gccattgagt acgcgaagaa atataacgta ccggtggtgc
    10741 tgacgctggg caccaagttt gtcattgccg agaatccgca gtggtggcag caattcctca
    10801 aagaccacgt ttctatcctt gcgatgaacg aagatgaagc cgaagcgttg accggagaaa
    10861 gcgatccgtt gttggcatct gacaaggcgc tggactgggt agatctggtg ctgtgcaccg
    10921 ccgggccaat cggcttgtat atggcgggct ttaccgaaga cgaagcgaaa cgtaaaaccc
    10981 agcatccgct gctgccgggc gctatagcgg aattcaacca gtatgagttt agccgcgcca
    11041 tgcgccacaa ggattgccag aatccgctgc gtgtatattc gcacattgcg ccgtacatgg
    11101 gcgggccgga aaaaatcatg aacactaatg gagcggggga tggcgcattg gcagcgttgc
    11161 tgcatgacat taccgccaac agctaccatc gtagcaacgt accaaactcc agcaaacata
    11221 aattcacctg gttaacttat tcatcgttag cgcaggtgtg taaatatgct aaccgtgtga
    11281 gctatcaggt actgaaccag cattcacctc gtttaacgcg cggcttgccg gagcgtgaag
    11341 acagcctgga agagtcttac tgggatcgtt aagttatcgt cggttcgtag gccagataag
    11401 gcgttcacgc cgcatctggc atttggctct cgatgcctga tgcgacgctg gcgcgtctta
    11461 tcatgcctac atatttttca tattttacat ccggcaacca ccgtttaccc cgtcaccacc
    11521 tcacccgccg gtggcgtttc cagcagttcc agcatggtac gggcgatttc acgctcgccc
    11581 atcactacct gattcgcacc acgttcggtg atatacgcca cttcatcgtc ataatgggcg
    11641 cgggcaataa tctcaatatc cggatttttc acgcgggcag atgccacaat ctcacccgct
    11701 tcataaccgt tgggaatcgt caggatcagc cattttgcac attccagatg cgccagttgc
    11761 ataatttctt cgttcgccgc attgcccaat actgcgcgga ccccgcgctc tcgcagctca
    11821 tcaacacggg ttcgtgacgt ctcaatcacc accagcggaa tatcagaggc gagcaatttc
    11881 tcccccagca ggctgcctac acgaccgtaa cccaccagta gcgcatggtt gcaaatatcc
    11941 actgggatct gcttctcttc ttcgattgcc tcttccagcg tctgctcttc cagcgtttcg
    12001 gtcttcgcca gatatttctc cagtagtgcg aacagtaccg ggttgagcat aatcgacagg
    12061 atcgcccctg ccagtaccag gttttgtccg gcctgcggca gtaaattcaa tgccattccc
    12121 agtcccgcca ggataaacgc gaactcacca atctgcgcca ggctggcggc gatggttaat
    12181 gccgtacgtt gggagtgacc aaacagtcgc accaggaaaa atgcggctaa cgacttacca
    12241 aacagaataa tcgccagcgt cgccagcact gccagcggtt gctgaatcag aattaacgga
    12301 tcaaacaaca tcccgacgga gacaaaaaac agcaccgcaa acgcgtcgcg caatggcagc
    12361 gtatcgtggg cggcacggtg actcagttca gactcgttca gtaccatccc ggcaaagaac
    12421 gcaccgagtg caaaggagac atcaaacagc tctaccgcac caaaggcaat ccctaacgcc
    12481 agcgccagca ccgacagggt aaacagctcg cgagaaccgg ttgccgcgct gcgtgccata
    12541 atccacggca ccagacggcg acctaccagc atcataatgg cgataaatgc gatcactttg
    12601 ccgatggtga tccccatatc gactgcaaga gtggcaaagc ccacatcgcc ctgttccatc
    12661 attcctgcca ctgcgggcag caacaccagc gtcagaacca ttaccaggtc ttccacaatc
    12721 aaccaaccga tggcgatttg cccacgctga ctgtcaatta attgccgttc ttcaagtgcg
    12781 cgcagtaaca ccacggtact ggcggtggaa agacataaac cgaacacgat accggtcatt
    12841 aacgaccagc ccagcacggc ggagagcgcc atacccagca gcgtcgccac ggctatctgg
    12901 gcgatcgcgc cgggaatggc gatggacttt accgccatca aatccttgag cgaaaaatgc
    12961 aaaccgacgc cgaacatcag caaaataacg cccagttcag ccagttccgg cgcaagcttg
    13021 gtatcggcaa caaagcccgg agtgaatggt cctgccagca cacccgctaa cagatatccc
    13081 accagaggag aaatacgtag tttattggcc agcatgccga ggataaaggc gagcacaagg
    13141 ccgccaacaa tggtggtgat aagcggggtg gcgtgatgca ttccgtctcc ttttcctggt
    13201 ggttattgtc catttttggc cgggaaaacc aaaattacag
//
LOCUS       DAZFQD010000143        13196 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.143, whole genome shotgun sequence.
ACCESSION   DAZFQD010000143 DAZFQD010000000
VERSION     DAZFQD010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13196
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.143"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..297
                     /locus_tag="ACHKTW_004202"
     CDS             <1..297
                     /locus_tag="ACHKTW_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HFO6465122.1"
                     /translation="QGFIRFHIPLWVRRTVTMLPSFIVILMGLDPTRILVMSQVLLSF
                     GIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWVIVVLVVALNIWLLVGTALGL"
     gene            complement(438..764)
                     /gene="ypeC"
                     /locus_tag="ACHKTW_004203"
     CDS             complement(438..764)
                     /gene="ypeC"
                     /locus_tag="ACHKTW_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein YpeC"
                     /protein_id="HFO6465123.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(879..2135)
                     /locus_tag="ACHKTW_004204"
     CDS             complement(879..2135)
                     /locus_tag="ACHKTW_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HFO6465124.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASALQNLLWQR
                     LPGTLGIAQDSPLWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPG
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMNQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            2339..3304
                     /gene="glk"
                     /locus_tag="ACHKTW_004205"
     CDS             2339..3304
                     /gene="glk"
                     /locus_tag="ACHKTW_004205"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - D-glucose binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HFO6465125.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDNPGLLGSGAHLRQTLGHIL"
     gene            3523..3849
                     /locus_tag="ACHKTW_004206"
     CDS             3523..3849
                     /locus_tag="ACHKTW_004206"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HFO6465126.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQALEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAVGIIKEIEEM
                     IASEQQ"
     gene            3871..5118
                     /locus_tag="ACHKTW_004207"
     CDS             3871..5118
                     /locus_tag="ACHKTW_004207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFO6465127.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAVI
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            5133..6218
                     /gene="ypdF"
                     /locus_tag="ACHKTW_004208"
     CDS             5133..6218
                     /gene="ypdF"
                     /locus_tag="ACHKTW_004208"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFO6465128.1"
                     /translation="MTLLASLRDWLKAQQLDAVLLSSRQNKQPHLGISTGSGYVVISR
                     ESAHILVDSRYFVEVEARAQGYQLHLLDATNTLTTIVNQIIADEQLQTLGFEGQQVSW
                     ETAHRWKSELNAKLVSATPDVLRQIKTPEEVEIIRLACGIADRGAEHIRRFIQAGMSE
                     REIAAELEWFMRQQGAEKASFDTIVASGWRGALPHGKASDKIVAAGEFVTLDFGALYQ
                     GYCSDMTRTLLVNGEGVSAESHPLFDVYQIVLQAQLAAISAIRPGVRCQQIDDAARRV
                     ITEAGFGDYFGHNTGHAIGIEVHEDPRFSPRDTTTLQPGMLLTVEPGIYLPGQGGVRI
                     EDVVLVTPQGAEVLYAMPKTVLLTGEA"
     gene            6218..7255
                     /gene="ypdE"
                     /locus_tag="ACHKTW_004209"
     CDS             6218..7255
                     /gene="ypdE"
                     /locus_tag="ACHKTW_004209"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFO6465129.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEGADRLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQPVRITTR
                     EECKIPGLLDGDRQGNDVSAMRVDIGARSYDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLGCYLLVTLLRELHDAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYSAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVAAEIGVPLQAD
                     MFSNGGTDGGAVHLTGTGVPTVVMGPATRHGHCAASIADCRDILQMQQLLSALIQRLT
                     RETVVQLTDFR"
     gene            7280..9775
                     /gene="ptsP"
                     /locus_tag="ACHKTW_004210"
     CDS             7280..9775
                     /gene="ptsP"
                     /locus_tag="ACHKTW_004210"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFO6465130.1"
                     /translation="MLTIQFLCPLPNGLHARPAWELKEQCSQWQSEVTFINHRQNAKA
                     DAKSSLALIGTGTLFNDSCSLNISGSDEEQARRVLEEYIQVRFIDSDSVQPTQAELTA
                     HPLPRSLSRLNPDLLYGNVLASGVGVGTLTLLQSDSLDSYRAIPASAQDSTRLEHSLA
                     TLAEQLNQQLRERDGESKTILSAHLSLIQDDEFAGNIRRLMTEQHQGLGAAIISNMEQ
                     VCAKLSASASDYLRERVSDIRDISEQLLHITWPELKPRNNLVLEKPTILVAEDLTPSQ
                     FLSLDLKNLAGMILEKTGRTSHTLILARASAIPVLSGLPLDAIARYAGQPAVLDAQCG
                     VLAINPNDAVSGYYQVAQTLADKRQKQQAQAAAQLAYSRDNKRIDIAANIGTALEAPG
                     AFANGAEGVGLFRTEMLYMDRDSAPDEQEQFEAYQQVLLAAGDKPIIFRTMDIGGDKS
                     IPYLNIPQEENPFLGYRAVRIYPEFAALFRTQLRAILRAASFGNAQLMIPMVHSLDQI
                     LWVKGEIQKAIVELKRDGLRHAETITLGIMVEVPSVCYIIDHFCDEVDFFSIGSNDMT
                     QYLYAVDRNNPRVSPLYNPITPSFLRMLQQIVTTAHQRGKWVGICGELGGESRYLPLL
                     LGLGLDELSMSSPRIPAVKSQLRQLDSEACRELARQACECRSAQEIEALLTAFTPEED
                     VRPLLALENIFVDQDFSNKEQAIQFLCGNLGVNGRTEHPFELEEDVWQREEIVTTGVG
                     FGVAIPHTKSQWIRHSSISIARLAKPIDWQSEMGEVELVIMLTLGANEGMNHVKVFSQ
                     LARKLVNKNFRQSLFAAQDAQSILTLLETELTF"
     gene            complement(9778..10635)
                     /locus_tag="ACHKTW_004211"
     CDS             complement(9778..10635)
                     /locus_tag="ACHKTW_004211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO6465131.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNPQLLGRVWFASQPASLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRMVEGEMLFIPARAANLPINNKPVMLLSLVFAPT
                     WLGLSFYDSRTTSLLHPARQIQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRNVVNMPPGNSQPRGDFLYHSICNWVQDNYAQPLTRESVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGFPDSDYFCRVFRRQFGLTP
                     GEYSARFQG"
     gene            complement(10648..11382)
                     /gene="ypdB"
                     /locus_tag="ACHKTW_004212"
     CDS             complement(10648..11382)
                     /gene="ypdB"
                     /locus_tag="ACHKTW_004212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HFO6465132.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            complement(11397..13094)
                     /gene="ypdA"
                     /locus_tag="ACHKTW_004213"
     CDS             complement(11397..13094)
                     /gene="ypdA"
                     /locus_tag="ACHKTW_004213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     YpdA"
                     /protein_id="HFO6465133.1"
                     /translation="MHEIFNMLLAVFDRAALMLICLFFLIRIRLFRELLHKSAHSPKE
                     LLAVTAIFSLFALFSTWSGVPVEGSLVNVRIIAVMSGGILFGPWVGIITGVIAGIHRY
                     LIDIGGVTAIPCFITSILAGCISGWINLKIPKAQRWRVGILGGMLCETLTMILVIVWA
                     PTTALGIDIVSKIGIPMILGSVCIGFIVLLVQSVEGEKEASAARQAKLALDIANKTLP
                     LFRHVNSESLRKVCEIIRDDIHADAVAITNTDHVLAYVGVGEHNYQNGDGFISPTTRQ
                     AMNYGKIIIKNNDEAHRTPEIHSMLVIPLWEKGVVTGTLKIYYCHAHQITSSLQEMAV
                     GLSQIISTQLEVSRAEQLREMANKAELRALQSKINPHFLFNALNAISSSIRLNPDTAR
                     QLIFNLSRYLRYNIELKDDEQIDIKKELYQIKDYIAIEQARFGDKLTVIYDIDEEVNC
                     CIPSLLIQPLVENAIVHGIQPCKGKGVVTISVAECGNRVRIAVRDTGHGIDPKVIERV
                     EANEMPGNKIGLLNVHHRVKLLYGEGLHIRRLEPGTEIAFYIPNQRTPVASQATLLL"
BASE COUNT         3092 a         3392 c         3602 g         3110 t
ORIGIN      
        1 cagggattca ttcgcttcca tatcccgctg tgggtgcgtc gtacagtcac catgttgccg
       61 tcatttattg tcattctgat gggattagat ccgacacgga ttctggttat gagtcaggtg
      121 ctgttaagtt ttggtatcgc cctggcgctg gttccactgc tgattttcac cagtgacagc
      181 aagttgatgg gcgatctggt gaacagcaaa cgcgtaaaac agacaggctg ggtgattgta
      241 gtgctggtcg tggcgctgaa tatctggttg ttggtgggga cggcgctggg attgtagttg
      301 aatgagcgtc gcatctggca ctattggcgg atgcggcgta aacgccttat ccgccctacg
      361 cgttaaagag ccggtttgta ggcctgataa gactcgctag cgtcgcatca ggcattgtgt
      421 tccattggcg aatgtgttta atggcggtgt ccatgtccgc ggccttttcc gcgatgatcg
      481 tcacgatcgc gccagccttc acgatagcca cgctcatacg ctttacgctt atcccagcca
      541 cgatgatagc cattatcatg acgccaccag cggtttttac gccactcata attgcgatgc
      601 cagtagtcac ggtcgcgcca gtgaccaccg tcccagtaat taccgtaatg atcgcgatcg
      661 ccaatttgta atttgattga tggcagtagg gtgatttcac ctgcgtttgc tgcaagcggg
      721 gtaaatgcca tcagggcggc cgccagaaac agtgacctga acattgttat tctccttcac
      781 gatcgaagcc gtcagcggcc tgttaacgca atattacggg gaggtaaagc cccgtatcat
      841 cgccataact cttaaatcat aaaggagagc attttttgct aaacgttatc gtggggtggc
      901 tgttgcttcg gacgattgac catcatcatc ggcttacctg ctaataacag ccatgccgga
      961 agcatgacga tacagagcag cggtagcaat gtggtattgg gtacaacgac cgccgccata
     1021 aaaagactta accagccatc gcgtgttacc accagcacga tgccgagaat agcgcaagaa
     1081 acggttattg ccgctggtac ggcgggtacg tgctcatgca gcatcaaccc taatgccacg
     1141 ccgacaaaca ctgccgggaa gattcgccca ccgcgaaagc cactggcggc agcaacgacc
     1201 agggcggcaa gtttaattac cgccagcaaa aagtaatcgc tggtgctgaa agcctgattt
     1261 gccaccatct gctgcatctc atccagccct ttaaacagcg aaactggtcc accaataacc
     1321 cccagaatac cgagaataaa tccgccaata cccagcacga gcaccggatt tttcatttga
     1381 ttcatcatcg cgtgcaaccg tggtaagcac catacggcga ccatccctgc tgcgatggca
     1441 atcgccgcga caattgcacc gctgagaata tcggtcattt ccatctgtcc gtaatgcgca
     1501 atgggcagtg aaaaatgggg atggaaaaat aatccggtag taagtgcacc agctgctgcc
     1561 gccattaatg gcgcaaaaag acgatcccat agcggaactt cactactgcc atttaaggtt
     1621 tgcgaaaata tcaacgccgc cgcaacaggt gtaccaaaca gcgcgccgat ggttccggca
     1681 gaggctaaaa tagtccactc cattcggttg acgcgcggta acagacgagc gccaatggca
     1741 accgcaaggg cgatattgac ggtcatgatc ggatgttccg gccccaggct gacgccgcca
     1801 gcaagaccga gaattaatgc tacgataagt cctggtagcg cagagggcgg aactggtgcg
     1861 ccgatcagcg gttcacaggc ggggtctggt ccggcatgac cctggctgaa acggataacc
     1921 aaccccaccg caatacccgt tagcgttaat acaccgatga tccagagagg tgaatcctgg
     1981 gctatcccca gagttcccgg cagtcgttgc cagagcaaat tctgtaatgc cgaggcgatt
     2041 ttcatcacca caatcagaat aagactggac gcaatcccaa ttgccacggc ggggagcgat
     2101 aataacaaca tggttctggc tcgcggatgg agcatgttct attccttatg cggggtcaga
     2161 tacttagttt gcccagcttg caaaaaggca tcgctgcaat tggtgctgaa acgataaagt
     2221 aaatgtgtga cccagatcga tatttacagg gagcctgcct ttccggcgtt gttgttatgc
     2281 ccccaggtat ttacagtgtg agaaagaatt attttgactt tagcggagca gttgaagaat
     2341 gacaaagtat gcattagtcg gtgatgtggg cggcaccaac gcacgtcttg ctctgtgtga
     2401 tattgccagt ggtgaaatct cgcaggctaa gacctattca gggcttgatt accccagcct
     2461 cgaagcggtc attcgcgttt atcttgaaga acataaggtc gaggtgaaag acggctgtat
     2521 tgccatcgct tgcccaatta ccggtgactg ggtggcaatg accaaccata cctgggcgtt
     2581 ctcaattgcc gaaatgaaaa agaatctcgg ttttagccat ctggaaatta ttaacgattt
     2641 taccgctgta tcgatggcga tcccgatgct gaaaaaagag catctgattc agtttggtgg
     2701 cgcagaaccg gtagaaggta agcctattgc ggtttacggt gccggaacgg ggcttggggt
     2761 tgcgcatctg gtccatgtcg ataagcgttg ggtaagcttg ccgggcgaag gcggtcacgt
     2821 tgattttgcg ccgaatagtg aagaagaggc cattatcctc gaaatactgc gtgcggaaat
     2881 tggccatgtt tcggcggaac gcgtgctttc tggccctggg ctggtgaatt tgtatcgcgc
     2941 aattgtgaaa gctgacaacc gcctgccaga aaatctcaag ccaaaagata ttaccgaacg
     3001 tgcgctggct gatagctgca ccgattgccg ccgcgcgttg tcgctgtttt gcgtcattat
     3061 gggccgtttt ggcggcaatc tggcgctcaa tctcgggaca tttggcggcg tgtttattgc
     3121 gggcggtatc gtgccgcgct tccttgagtt cttcaaagcc tctggtttcc gtgccgcatt
     3181 tgaagataaa gggcgcttta aagaatatgt ccatgatatt ccggtgtatc tcatcgtcca
     3241 tgacaatccg ggtcttctcg gttccggtgc acatttacgc cagaccttag gtcacattct
     3301 gtaaatcctt cctgttatat cgggaggtaa ctctcccgat aatcttttaa atcatacagt
     3361 taattcaatt tttctttgtg tcccctcaca aggccgacct gcgtcacact tccatacagc
     3421 gggattaatt ctccagtaaa tgcattattt gtctggtaac ggcgatttgt tttgcacgtt
     3481 cataatttca ctcgtcaact atcactgaac gaggaattaa cgatgagtaa gaaactgatt
     3541 gccttatgtg cctgcccgat gggcctggct cacactttta tggccgctca ggcgctggaa
     3601 gaagcggcgg tagaagccgg ttatgaagtg aaaattgaaa cccagggcgc ggacggcatc
     3661 cagaatcgcc tgacggcgca ggatatcgcc gaagcgacca tcatcatcca ttccgtggca
     3721 gttaccccgg aagataacga acgtttcgaa tcacgcgacg tttatgaaat cactttgcag
     3781 gacgcaatta aaaacgctgt aggcatcatc aaagaaatcg aagagatgat tgcctctgaa
     3841 caacagtaat attcgacagg gataattgtt atggccatta aaaaacgcag tgcaaccgtt
     3901 gtgcctggcg catccggtgc ggcagcggca gttaagaatc cgcaggcctc taaaagcagc
     3961 ttctggggtg aactcccgca gcatgtgatg tcagggattt cacgcatggt gccgacctta
     4021 attatgggcg gtgtgatcct cgctttcagc cagctgattg cttatagctg gcttaaaatt
     4081 cctgctgaga tcggcatcat ggatgctctt aatagcggga aattctccgg tttcgacctc
     4141 tccttactga aatttgcctg gctgtcgcag tccttcggcg gcgtgttgtt tggctttgct
     4201 attccgatgt ttgccgcttt tgtggcgaac tctatcggcg gcaaactggc gttcccggct
     4261 ggttttatcg gcggcttgat gtctacccag ccgacgcaac tgctgaactt cgatcccagc
     4321 actatgcaat gggcgacctc ttcgccggtg ccgtccacct tcattggtgc gctgattata
     4381 tctatcgttg ctggttacct tgtgaaatgg atgaaccaga aaatccagtt accagatttc
     4441 ctgttagcgt tcaaaaccac atttttgcta ccgattcttt ccgccatttt tgtcatgctg
     4501 gcgatgtact atgtcatcac cccatttggt ggctggatca acggcggtat ccgtaccgtg
     4561 ctgactgccg caggtgagaa gggcgcgctg atgtatgcaa tggggattgc cgcggcgacc
     4621 gcaatcgacc ttggtggccc aatcaacaaa gcggcaggtt tcgttgcctt cagctttacc
     4681 accgaccacg tattaccggt caccgctcgt tctatcgcta tcgttattcc tccgattggt
     4741 ctgggtctgg cgaccattat tgaccgtcgt ttaaccggca aacgcctgtt caacgctcaa
     4801 ctttatccgc agggtaagac cgcgatgttc cttgccttta tggggattag tgagggtgcg
     4861 attccgtttg cgctggaaag ccccatcacc gccattccgt cgtatatggt cggcgcgatt
     4921 gtcggctcaa ccgccgctgt ctggctgggc gcagtgcaat ggttcccgga atccgctatc
     4981 tgggcatggc cgctggtcac taacctgggc gtctatatgg cggggatcgc gctgggggca
     5041 gtcataactg cgctgatggt ggtgttcctg cgtctgatga tgttccgtaa aggcaaattg
     5101 ttaatcgata gcctgtaaga aggaaaatcc agatgacatt actcgcttcg ctgcgcgact
     5161 ggcttaaggc gcaacaactg gatgcagtgc ttctctcctc acggcagaac aaacagccgc
     5221 atctggggat ctccaccgga tcaggctatg tggttattag ccgtgaaagt gcgcacattc
     5281 tggtagattc gcgctatttc gttgaggtgg aagcccgtgc gcaaggctac cagctgcatt
     5341 tgcttgacgc gacgaacacg cttaccacta tcgtcaatca aatcattgcc gatgagcagt
     5401 tgcaaacgct cggttttgaa ggccagcagg tgagttggga aaccgcgcac cgctggaagt
     5461 ctgaactcaa tgcgaaactg gttagcgcca cgccggatgt gctgcggcaa atcaaaacgc
     5521 cagaggaggt ggagataatc cgcctcgcct gtgggattgc cgatcgcgga gcggagcata
     5581 ttcgccgctt tattcaggcg gggatgagcg agcgcgagat agccgctgaa ctggagtggt
     5641 ttatgcgcca gcagggcgca gaaaaagcct cttttgacac cattgtcgcc agcggctggc
     5701 gcggggcgct gccgcacggc aaagccagcg acaagattgt tgcagcgggc gagtttgtca
     5761 ctctcgattt tggtgcgctg tatcagggct actgctctga tatgacgcgc accttgctgg
     5821 tgaatggcga aggggtgagc gccgaatctc acccgctgtt tgacgtctat cagattgtcc
     5881 tgcaggcaca gctcgcggca atctctgcaa ttcgccccgg cgtgcgctgc cagcagattg
     5941 acgacgccgc gcgccgggtc attaccgagg ctggatttgg cgactatttc ggtcataaca
     6001 ctggtcacgc tatcggcatt gaagtccatg aagatccgcg tttttcaccg cgggacacca
     6061 cgacgctaca gccaggcatg ttgctgaccg tggagccggg gatttatttg ccagggcaag
     6121 ggggcgtgcg catcgaagat gttgtgctgg ttaccccgca aggcgcagaa gtgctctacg
     6181 ccatgccgaa aacagtgttg ctcacgggag aggcataatg gatttatcgc tattaaaagc
     6241 gttgagcgag gcagatgcga tcgcctcctc ggaacaggaa gtgcggcaga tcctactgga
     6301 aggagcggat cgcctgcaaa aagaagtgcg atttgatggt ctgggatcgg tgctgatccg
     6361 cctcaatgaa tcgacaggtc cgaaggtgat gatctgcgcc catatggacg aagtgggatt
     6421 tatggtgcgc agcatctccc gcgaaggcgc tattgacgtg ctgccggttg gcaacgtgcg
     6481 catggctgcc cgccagctgc agccggtgcg catcaccacc cgtgaagagt gcaaaattcc
     6541 aggcctgctt gacggcgacc ggcaggggaa tgacgtcagc gccatgcgcg tggacattgg
     6601 tgcgcgctcg tatgacgaag tgatgcaggc gggaattcgt ccaggcgatc gcgtcacctt
     6661 tgatacaact tttcaggttc tccctcacca gcgggtgatg gggaaagcct ttgatgaccg
     6721 cctcggttgc tatctgctgg tgacgttact acgtgagctg cacgacgccg aactgcctgc
     6781 ggaggtgtgg ctggtggcaa gttccagcga agaggtggga ttacgcggcg ggcaaactgc
     6841 cacccgcgcg gtgtcgccgg atgtcgccat tgtgcttgat accgcctgct gggcgaaaaa
     6901 ctttgattat agcgcggcta accatcgcca gattggtaac gggccgatgc tggtgttaag
     6961 cgacaagtcg ctgattgcgc cgccaaaact taccgcctgg atcgaaaccg tcgcggcaga
     7021 aattggcgtg ccgttgcagg cggatatgtt cagcaacggc ggcacggacg gcggagcggt
     7081 gcatttaacc ggcaccggcg tgcccacggt ggtgatgggg ccagcgacgc gccacggaca
     7141 ttgtgcggca tcgattgccg attgccgcga cattttgcag atgcagcaac ttttatctgc
     7201 ccttattcaa cgtcttaccc gtgagacggt tgttcaactg acggatttca gatgatctcc
     7261 tgattaaccc ggagcggtta tgttaacgat tcaatttctc tgtcctttgc ctaacggtct
     7321 gcatgctcgt ccggcgtggg aacttaaaga acagtgcagc cagtggcaaa gtgaagtcac
     7381 ctttattaac catcgccaga acgcaaaggc cgatgcgaaa agttcgctgg cgctgattgg
     7441 caccggcacc ctatttaatg acagttgcag cctgaacatt agcggcagcg atgaagagca
     7501 ggcgcggcgc gtgctggaag agtacatcca ggtgcgcttt atcgacagcg acagcgttca
     7561 gcctacgcag gcagaactga cggcgcatcc gctgccgcgt tcattaagcc gccttaaccc
     7621 ggatttactg tacggcaatg tgctggcaag cggcgtcggc gtgggtacgc tgaccctgtt
     7681 acaaagcgac agcctcgaca gttatcgggc aatccccgcc agtgcgcaag attccacccg
     7741 gctggagcac agcctggcaa cgcttgccga gcaactgaac cagcaattgc gtgagcgtga
     7801 cggcgaaagc aaaactatcc tcagcgctca tttgtcgctg attcaggatg atgaatttgc
     7861 aggcaatatc cgtcgcctga tgacagaaca gcatcaggga ctgggggcgg cgatcatcag
     7921 caatatggag caggtttgcg ccaagctttc tgcttctgcc agcgattatc tgcgcgaacg
     7981 tgttagcgac attcgcgata ttagcgaaca gttgctgcat atcacctggc cggaactgaa
     8041 gccgcgcaac aatctggtgc tggaaaaacc gaccattctg gtggctgaag atttaacccc
     8101 aagccagttt ttgagccttg atttgaaaaa tcttgcgggc atgattctgg agaaaaccgg
     8161 gcgcacctcg cataccctga ttctggcccg cgcctcggcg atcccggtac tgagcggcct
     8221 gccgctggat gcgattgccc gttatgccgg gcaaccggcg gtgcttgacg cccagtgcgg
     8281 cgtgctggcg attaacccga atgacgcggt gagcggttat tatcaggttg cgcagacgct
     8341 ggcggataaa cgccaaaaac aacaggcgca ggctgccgcg cagctggcct attcccgtga
     8401 taacaagcgt attgatatcg cggcgaatat tggcaccgct ctggaagcac caggcgcgtt
     8461 tgccaacggc gcggaaggcg tcgggctgtt ccgtaccgaa atgctgtata tggatcgtga
     8521 cagcgcgccg gacgagcagg agcaatttga agcctaccag caggtgctac tggcagcggg
     8581 cgacaagccg attatcttcc gcacaatgga catcggaggc gataaaagca ttccttatct
     8641 gaatattccc caggaagaga acccgttcct cggctatcgc gcggtacgta tttacccgga
     8701 atttgctgcc ctgttccgca ctcaactacg ggcgattttg cgcgctgcca gttttggcaa
     8761 cgcccagttg atgatcccga tggttcacag cctcgatcag atcttatggg tgaagggcga
     8821 gatccaaaaa gcgatcgttg agcttaagcg cgatggcctg cgtcatgcag agacgattac
     8881 tctgggtatc atggtggaag tgccgtcggt gtgttacatc atcgaccact tctgcgatga
     8941 ggtcgatttc ttcagtatcg gttctaacga tatgacccag tatctgtatg cggtcgatcg
     9001 taataacccg cgcgtatcgc cgctgtataa cccgattacg ccatcgttcc tgcgcatgtt
     9061 gcagcagatt gttaccactg cgcatcagcg gggcaaatgg gtaggcattt gcggtgaact
     9121 gggcggtgaa agccgttatc tgccgctact gcttgggctg ggcctggatg agctgagtat
     9181 gagtagcccg cgtattccgg cggtgaaaag ccagcttcgt caactggata gcgaggcgtg
     9241 tcgggaactg gcgcgtcagg catgtgaatg ccgcagtgcg caggaaattg aagcgttact
     9301 caccgccttt acgccggaag aagacgttcg cccactgctg gcgctggaga atatctttgt
     9361 tgatcaggat tttagcaata aagagcaggc gatccagttc ctgtgcggca acctcggcgt
     9421 taacgggcgc actgaacatc cgtttgagct ggaagaagat gtctggcagc gggaagagat
     9481 tgttaccacc ggcgttggtt ttggcgtagc gatcccgcac accaaatctc agtggatccg
     9541 tcattccagt atcagcattg cccggctggc gaaaccgatt gactggcagt cagaaatggg
     9601 cgaagtcgaa ctggtgatca tgctgacgct gggtgctaac gaagggatga atcatgtgaa
     9661 agtcttctcg cagctggcgc gtaaactggt gaataaaaac ttccgccagt cgttgtttgc
     9721 cgcgcaagat gcacaaagta tcctgacgct gctggaaaca gaattaacct tctgacgtta
     9781 gccctgaaaa cgggcgctgt actctcccgg cgtcagacca aactgacgcc ggaaaacgcg
     9841 acaaaaatag tcgctatccg gaaaaccgca acgctgtgcc acttcatgaa ttgacagatg
     9901 atatttctgc aaaatcatcc tcgccttcgc cattcgcacc caacgcacat actcgataaa
     9961 acgcatcgtt ccatgctgag caaacagttt tgacagatga ttgggcgtga tattaaaaaa
    10021 ctgcgccacg ctctcgcggg tgagcggctg ggcataatta tcctgaaccc agttacaaat
    10081 gctgtgatag agaaaatcgc cgcgcggctg cgaattgccc ggcggcatat tcaccacgtt
    10141 acggcaaaga tgcagcaaac ttaacaccag tggctgaatg atattctgct ccagcggcga
    10201 acggctaaga tgggtgaggg cggtaagcat cgcttcacct tcaccgcgtt gcaggctggg
    10261 aagctggatc tggcgagcag gatgcaacaa cgacgtggtg cggctatcgt aaaacgataa
    10321 cccaagccag gtcggtgcga acaccaggct taacagcatc accggtttgt tgttgatcgg
    10381 taaattagcc gcgcgtgccg gaataaacag catttctcct tccaccattc gctgctgctt
    10441 tgcttccagc aggttgccgt attcgccgcg cagcacgata tccagacggg gaaaatcaat
    10501 acataaactg cccaccggta acgaggcagg ctggctggca aaccagacgc gccccagtag
    10561 ttgcgggtta agcaccaggc cgctgaacag atcggcgaaa aattgttgat cggcgggcaa
    10621 gcctggtgct ttcatgaaag ttcccgatta aagatgcatt aactggcgaa attctttcac
    10681 tttgctgcgg ctgaccggca cttcaaaatc cagatctttc agtcgcagaa tgtaggtgtt
    10741 attaaaccac ggttcgattt cgcgtatttt gttcagattg acacaaaatg agcgatggca
    10801 gcggaaaaaa tgcgacggcg gcagtttgct gcaaaattcg gtaatgttca tcggcattac
    10861 gtaggattca cgccgcgtat agacaaaggt cattttctcg tgcgcttcgg cgtaatagat
    10921 atcgttaatt ggcgtgacga ttattcgctc atctttcacc agattaatgg tgtcattctc
    10981 acgcgttacc gtcgcggcag gcgtactgct ggtctgctgt tgttgccagg ccgcttccag
    11041 tttttgcagc atcccggtaa tacgtgactc ctgatacggt ttgagaatgt agtcaaacgc
    11101 ctccagttca aacgcttcta ccgcatgttc tttccacgcg gtgatgaaca caataaacgg
    11161 tttatgggcg aactggctga tgttttgcgc cagcaacacg ccatccagcg acggaatatt
    11221 gatatccaga aaaatggcgt cgacgcggtt atgctgcaaa aacttcaaca cgtccagacc
    11281 gtcgtcaaag gtgccgacaa tctccatctg gctgtgctct ttaattagcc agctcagttc
    11341 ctgttgtgcc aggaattcgt cttcaacaat gatgactttc acaatatcac tccggctcaa
    11401 agcaataacg tagcctgtga ggcgactggg gtgcgttggt taggaatgta aaacgcaatt
    11461 tccgttcctg gttccaggcg gcggatatgc agcccctcgc catacaataa cttcacgcga
    11521 tgatgaacat tcagcaggcc aattttattg cccggcattt cattcgcttc gacccgctca
    11581 atcacctttg gatcgatgcc gtgcccggta tcccgcaccg caatgcgtac ccgattgccg
    11641 cactctgcaa cgctgatggt gacaacgcct ttacctttgc aaggctgaat accgtggaca
    11701 atggcattct ccaccaacgg ctggatcagc aggctgggaa tgcagcaatt cacctcttca
    11761 tcaatatcat agataaccgt cagcttgtcg ccaaagcggg cctgctcaat ggcaatatag
    11821 tctttaattt gatacagctc ttttttgata tcgatttgct catcgtcttt taattcaatg
    11881 ttatagcgca gataacgcga cagattaaag atcagctggc gagcagtatc cggattcaga
    11941 cggattgatg atgaaatagc gttcaaagcg ttaaacagga aatggggatt aattttgctt
    12001 tgcagggcgc gaagctctgc cttatttgcc atttcacgta gctgctcggc gcgtgaaacc
    12061 tccagttggg tggagatgat ttgcgacaga ccgaccgcca tttcctgtaa tgacgaggtg
    12121 atctgatgcg cgtggcagta gtaaattttc agcgttccgg tcacaacgcc tttctcccac
    12181 aatgggatca ccagcatgga atgaatctct ggtgtgcggt gggcttcatc attgttttta
    12241 atgatgattt ttccgtaatt catcgcctga cgggtagtcg ggctaatgaa gccatcgcca
    12301 ttctgatagt tatgttcacc cacgccaaca taggccagca catgatcggt attggtaatc
    12361 gccaccgcat cggcgtgaat gtcatcgcga ataatttcac aaactttgcg taatgactcg
    12421 ctattgacat ggcgaaacag aggtagcgtt ttgttggcga tatccagcgc cagcttggcc
    12481 tgccgcgcgg cgctggcctc tttttcgccc tcaacgcttt gcaccagaag cacaataaag
    12541 ccgatacaga cgctaccgag gatcattgga atgccgattt tagagacgat atcgatcccc
    12601 aacgcggtag ttggtgccca gacaattacc agaatcatgg tcaacgtctc acacaacatg
    12661 ccgccgagaa taccgacgcg ccagcgctgt gctttgggga ttttcaggtt tatccagcct
    12721 gatatacaac ccgccagaat gctggtgata aagcagggga tcgccgtcac gccgccgata
    12781 tcaattaaat accggtgaat acccgcaatc acgccggtaa tgatgcctac ccacgggcca
    12841 aacagaatcc cgccggacat caccgcgata atacgtacgt tcaccagcga gccttctacg
    12901 ggaacgccgg accaggtgct gaacagggcg aacagcgaga aaatggcggt aacggcgagc
    12961 aattcttttg gggagtgcgc cgacttgtgc aacagttcgc gaaacaggcg gatacggatg
    13021 agaaagaaca ggcagataag cattaacgct gcccgatcga agaccgccag cagcatgttg
    13081 aatatttcgt gcacgggtga ctctgaataa ggattaaacc tctatgatag gtaacctgaa
    13141 ggctgatgac cagcaggccg tttttgagga aaaaccataa atttaaaagg ggattg
//
LOCUS       DAZFQD010000144        13183 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.144, whole genome shotgun sequence.
ACCESSION   DAZFQD010000144 DAZFQD010000000
VERSION     DAZFQD010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13183
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.144"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            304..1134
                     /gene="yjiC"
                     /locus_tag="ACHKTW_004214"
     CDS             304..1134
                     /gene="yjiC"
                     /locus_tag="ACHKTW_004214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313311.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HFO6465134.1"
                     /translation="MSMPLSNALQSQIITDNHFLHHPKVDSELTRRYEYARLDTENIY
                     LLPLARGNNHNYDGKSVVEIRKLDISKEPWPFNYVTETCRESDGITTTGRMLYRNLKI
                     TTVLDEIYGGICKKAHAATELAEGLRLNLFMKSPFDPVEDYTVHEITLGPGCNVPGYA
                     GTTIGYISTLPASQAKRWTNEQPRIDIYIDQIMTVSGVANSSGFALAALLNANIELGN
                     DPIIGIEAYPGTAEIHAKMGYKVIPGDENAPLKRMTLQPSSLPELFELKNGEWNYIGK
                     "
     gene            complement(1275..2048)
                     /gene="uxuR"
                     /locus_tag="ACHKTW_004215"
     CDS             complement(1275..2048)
                     /gene="uxuR"
                     /locus_tag="ACHKTW_004215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HFO6465135.1"
                     /translation="MKSATSAQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDNSGSQNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(2263..3723)
                     /gene="uxuB"
                     /locus_tag="ACHKTW_004216"
     CDS             complement(2263..3723)
                     /gene="uxuB"
                     /locus_tag="ACHKTW_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HFO6465136.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRVLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GAQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMRYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTAAYQQLCERGARECVAAL"
     gene            complement(3804..4988)
                     /gene="uxuA"
                     /locus_tag="ACHKTW_004217"
     CDS             complement(3804..4988)
                     /gene="uxuA"
                     /locus_tag="ACHKTW_004217"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFO6465137.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAE
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            5328..6671
                     /gene="gntP"
                     /locus_tag="ACHKTW_004218"
     CDS             5328..6671
                     /gene="gntP"
                     /locus_tag="ACHKTW_004218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HFO6465138.1"
                     /translation="MHVLNILWVVFGIGLMLVLNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISAAILDPATGQLVGVNPALLVLAT
                     AAGSNTLTHINDASFWLFKGYFDLSVKDTLKTWGLLELVNSVVGLIIVLIISMVA"
     gene            complement(6914..7816)
                     /gene="fimH"
                     /locus_tag="ACHKTW_004219"
     CDS             complement(6914..7816)
                     /gene="fimH"
                     /locus_tag="ACHKTW_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HFO6465139.1"
                     /translation="MKRVITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPAVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGTVKYSGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIGVTFVYQ"
     gene            complement(7836..8279)
                     /locus_tag="ACHKTW_004220"
     CDS             complement(7836..8279)
                     /locus_tag="ACHKTW_004220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO6465140.1"
                     /translation="MQAADVTITVNGKVVAKPCTVSTTNATVDLGDLYSFSLMSAGAA
                     SAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQGTAQNIQLELQDDSGNTLNTG
                     ATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVISITYTYS"
     gene            complement(8352..8882)
                     /gene="fimF"
                     /locus_tag="ACHKTW_004221"
     CDS             complement(8352..8882)
                     /gene="fimF"
                     /locus_tag="ACHKTW_004221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HFO6465141.1"
                     /translation="MRNKPFYLLCAFLWLAVSHALAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAAKQFNNIGATTPVVPFCILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAASGLGIQLLNEQQNQIPLNAPSSALSWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(8892..10929)
                     /locus_tag="ACHKTW_004222"
                     /pseudo
     CDS             complement(8892..10929)
                     /locus_tag="ACHKTW_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313303.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
     gene            complement(10996..11721)
                     /gene="fimC"
                     /locus_tag="ACHKTW_004223"
     CDS             complement(10996..11721)
                     /gene="fimC"
                     /locus_tag="ACHKTW_004223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418736.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HFO6465142.1"
                     /translation="MSNKNVNVRKSQEITFCLLAGILMFMAMMVAGRAEAGVALGATR
                     VIYPAGQKQVQLAVTNNDENSTYLIQSWVENADGVKDGRFIVTPPLFAMKGKRENTLR
                     ILDATNNQLPQDRESLFWMNVKAIPSMDKSKLTENTLQLAIISRIKLYYRPAKLALPP
                     DQAAEKLRFRRSANSLTLINPTPYYLTVTELNAGTRVLENALVPPMGESTVKLPSDAG
                     SNITYRTINDYGALTPKMTGVME"
     gene            complement(11758..12297)
                     /gene="fimI"
                     /locus_tag="ACHKTW_004224"
     CDS             complement(11758..12297)
                     /gene="fimI"
                     /locus_tag="ACHKTW_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO6465143.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTLPGGNMQFQGVIIAETCR
                     IEAGDKQMTVNMGQISSNRFHAVGEDSAPVPFVIHLRECSTVVSEHVGVAFHGVADGK
                     NPDVLSVGEGPGIATNIGVALFDDEGNLVPINRPPANWKRLYSGSTSLHFIAKYRATG
                     RRVTGGIANAQAWFSLTYQ"
     gene            complement(12362..12910)
                     /gene="fimA"
                     /locus_tag="ACHKTW_004225"
     CDS             complement(12362..12910)
                     /gene="fimA"
                     /locus_tag="ACHKTW_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HFO6465144.1"
                     /translation="MKIKTLAIVVLSALSLSSAAALADTTTVNGGTVHFKGEVVNAAC
                     AVDAGSVDQTVQLGQVRTASLKQAGATSSAVGFNIQLNDCDTTVATKAAVAFLGTAID
                     ATHTDVLALQSSAAGSATNVGVQILDRTGAALALDGATFSSETTLNNGTNTIPFQARY
                     FATGAATPGAANADATFKVQYQ"
BASE COUNT         3257 a         3346 c         3163 g         3417 t
ORIGIN      
        1 aatatgaatt gataacatat caaatacatc aaaaatacca ttcacaggca ttattaacat
       61 agacctcaat atagcaacat caaattccaa ttagtaataa gatatgcttt ttaattgcat
      121 gaaaataatt ttaaatgagc gttttttgat actttagagt gtttcctacc ctttaatatc
      181 accatgtaga gttgatatca ctacaacaga aacatgcatg tcatttgttt acttcttttt
      241 tactacagaa aaatttatct atttttctaa acatcagtga aatactcata aaaggtaact
      301 attatgtcta tgccattaag caatgccctt cagagtcaaa ttattacaga taatcacttc
      361 cttcatcatc caaaagtcga cagtgagtta acacgtcgat atgaatatgc acgcctggat
      421 acggaaaata tctatcttct gcctcttgct cgtggcaata accataatta cgatggaaaa
      481 agcgttgtcg aaattagaaa acttgatatt agtaaagagc cctggccatt taattatgtg
      541 acagaaacat gcagagaatc cgatggtatt acaacaacag gacgtatgct ctatcgaaac
      601 ttgaaaatca ccacagtttt agacgaaata tatggtggta tatgtaaaaa ggcgcatgct
      661 gccacagagt tagcagaagg attacggcta aatttattta tgaagagccc tttcgatcct
      721 gttgaggact atacagttca tgaaataaca ttaggccctg gttgcaatgt tcctggttat
      781 gccggaacca cgataggata tatttcaacc cttccagctt ctcaggcaaa aagatggacc
      841 aatgagcagc caaggattga tatctatatt gatcagataa tgactgtctc aggggtggca
      901 aattcttccg gattcgccct tgccgcatta cttaatgcta atattgagtt gggtaatgac
      961 ccaataattg gtatagaagc ctatcccggt accgcggaga tccatgcaaa aatgggatat
     1021 aaagttatcc cgggtgatga aaatgcacca ctcaaacgta tgactctgca accgtcatct
     1081 cttccagaac tgtttgaatt aaaaaatggt gaatggaatt atattggtaa ataactacca
     1141 ggaacaatga gtactaatat tcaggttata cagatattta aacaataccc tgcaatttac
     1201 aaagtaatac tcattgcgac ttcttaattt aagaaagcgc aaggaacgtt tacccttgcg
     1261 cttattataa taagtcaggc gtcgacttta tccagcggcc atgaatcaaa cagatagcca
     1321 tcaaaataaa tatcgtcaac gttcgagaat tccagcagac gttgcttaac gttttccaga
     1381 tgctgccaca ttgccagctt cgccgctcgg gcatcttttt tgattaacgc ggcgaggatc
     1441 tgtttgtgat cgcccaacca ctctttgcga tacaggctgt catccagatg gctgtgcaac
     1501 tggatccaca ttggattgtt ttcccgccac tgccaggact gacggaacag ctccaccagc
     1561 atgctgttat gcgttgcttc ggcaatagcg agatggaact gcatgtcacc gctttcgctg
     1621 ctgcctggcg cactggaagc cagttcacgc tcttccagtt gcaatgcctg acgcattttg
     1681 acgatatctt cgcgggtagc ctgcaaagcg gcaaactcgg cgatgttgct ctccaataac
     1741 tgccgcgcct gtaacagctc aaaaggaccg gcatcgttgc agacgttggc atccggactg
     1801 tctgtgttct ggctgcctga gttatcaaga acatagatac cggcaccccg gcgtacttcc
     1861 accagccctt tgatctccag catgatcagc gcttcacgta ccaccgtccg cgtgacatca
     1921 agcatttctg caatttcacg ctccggcggc agccgttcgc cagggttgta cggcgtcttt
     1981 atgatcagat cgcggatcat cgccccgact tcctggtaag gtctttgcgc agaggtggca
     2041 gatttcatgg tgttgtgtcc gttaggtgcg gttaatcaaa tttttgccgg atgcggcgtg
     2101 aacgccttat ccggcctaca acgggcaatt ttacggcccc cgttaatctc aaactatacc
     2161 attagcactg gaagttggca cgtacaggtg ggcaaaaaaa cgaaaactgg tcaaccaaat
     2221 atcagtttga ttgaccagta agtctgtagg gtaatcagtt agttacagcg cagccacaca
     2281 ctcgcgcgca ccgcgttcgc acagctgctg atatgccgcc gtcactgcgc caacaaagtc
     2341 ggcattctgc ggcagatcat cggcaaaaat accgctcagg cccagcagcg ctttcacgcg
     2401 gtctgcgccc tgatactgcg cgttaatctt ctggaactcg gccagcatcg ggtcgaccac
     2461 gtcaatggca ttaccctgct catccacgcc ctgggtgtaa cgcatccagc cagccacgcc
     2521 cagcgccagg tgacgccagc taccgccgtt ttgcaggtgc agacgcaccg gatccagcag
     2581 acgctgcggt aacttctggc tgccgtccat tgcaatctgc caggtacggt gacgcagaga
     2641 tgggttgctg aaacgctcga tcagcagcgt cgcataggcg ttcaggtccg taccttccgg
     2701 catcgacagc gttggcgctt gttcctgcat catcagggca aaggccgctt tgcgataatc
     2761 cgggttagtc atggtgtcgg caatagtttc atagccgccg aggtaaccga ggtacgccag
     2821 gaaagagtgg ctgccgttca gcatacgcag cttcatcatt tcgaacggca caacgtctgc
     2881 aacgaactgt gcgcccactt tatcccagtc cgggcgacca ttaacgaagt tatcttcaat
     2941 cacccactga cggaacggtt cgcaggcaat ggcgcacggg tcgtaaacac ccagctggtc
     3001 agcaatttcc tgtaaggttt ctggcgtcgc cgccggaacg atgcggtcaa ccatggtgca
     3061 tgggaaggtg acgttctctt caatccatgc cgccagctgc ggatcgcgtg cctgagccag
     3121 tcccagtacc gcgaccttcg ccacatgacc gttttcacgc acgttatcgc aggacatcac
     3181 cgtaaacgct ttcaaccctt tttcacgacg cagacgcaag gcttcgacga tgtaaccaat
     3241 cgcggacttc ggcgcagtcg ggttttccag atcgtgcttg atcagtgggt tattgagatc
     3301 cagttgaccg cttgccgcat cagcgcagta gcctttttcc gtgaccgtta gagagacaat
     3361 cgccgtttgc ggacgcgcca tcgcgttgag aataccttcg cagccatcga tttccggatg
     3421 cagcgcttct ttcatcgaac cgataatttt cagctcggtg ctctctgcgc ctttttccgc
     3481 tacggtgtac agcagttgct gttttttcag gttttcgatc agcacgcggt cgttgcctgg
     3541 catcaggtta acttcgcaga tgccccagtc gctgtcggtg ctttccagca gatgatgggt
     3601 atacagcgcc tggtgcgcgc ggtgaaacgc cccgcaaccg agatgcacaa tgcgtgattc
     3661 cagacgagaa tgatcccatg acgggcgggc aaccggcaga ttgctgtcaa caatagtagt
     3721 catcgtaaac tccagaatcc gtcttcaaag agtgacggca aggaagaagg aaccggcacg
     3781 gcgcgcagcc atgccggtgg atattaacgg ctaaagaaag cgcgctggat cgccagttcg
     3841 acaccgcgaa cttcggccag gcctttcaga cgaccaattg cggagtaacc tgggttggtt
     3901 ttcttcttca ggtcgtccag catctgatga ccgtggtccg gacgcatcgg gatcaggtct
     3961 tctttgcctt ccgctttacg acggtgttct tcttcaacaa tcgctttcac cacttcgtac
     4021 atatcaacgt caccgttcag gtgcgccgct tcgtggaagg ttttcgggtt atcttcacgc
     4081 atggtggagc gcagatgggt gaagtaaata cgcggaccga actgcttgat catatcaacc
     4141 agatcgttgt cagcacgcac gccgtaggaa ccggtgcaca tggtaaaacc gtttgccatg
     4201 ctgtttacgg tatcaaccat ccactgcata tcttcaatgg tggaaacaat gcgcggcagg
     4261 ccgaggatcg ggcgcggcgg atcgtccggg tgaacagcca tacgcacgcc aacttcttcg
     4321 gcaaccggaa taatcgcttt caggaagacg gcaaagtttt cgcgcagttt ggctttgtcg
     4381 atatctttgt acagctccag gtgcttacgg aactggtcga gggtgtaccc ttcttccgcg
     4441 cccggcagac cagcgatgat gttacgggtc agacgcgctt tgtcttcgtc gctcatagtg
     4501 gcgaagcgtt cagctgcctg agcaatttct tcttcggtgt aatccgcttc cgcgcctgga
     4561 cgtttcagga tatgcatttc gaatgcagcg aattcgatct ggtcgaagcg cagagctttg
     4621 gagccgtctg gcagcacgta ttcgaggtca gtacgggtcc agtcgagcac cggcatgaag
     4681 ttgtagcaca cggtgcgaat gccacactgc gccaggttgc gcagagtctg ctgatagtta
     4741 gcgatccact gctcatagtt gccagtgtgg gttttgatat cttcgtgaat tggtacgctt
     4801 tctacgacag accacaccag gcctgcgtct tcaacgatcg ccttgcgttt gaggatctct
     4861 tctacggacc atacttcgcc gttcgggata tggtgcagcg cggtaaccac gccagttgcg
     4921 cccgcctgac ggacatcagc taaagaaacc ggatcgtttg ggccgtacca gcgccaggtc
     4981 tgttccatga tttttcctct ttcacatagt gggttccaca gcaaagagac gcggaaccct
     5041 ggagttgttt aggtatccgg atgaacaacc agaccgcagc cgtggcgttg catcggatga
     5101 gatggcgtat aagttctacc ttttggtaag acatgtcaac ccgaattctg aaattggtta
     5161 accacatcac aagaatttta ctctcctggt tgttgctgca aattgcagca aagggtaatc
     5221 gatggttatt gatcagagga tgtgacattc atcgcaacaa tggttgacca atttacataa
     5281 catgtcgccc aaaataacaa cggttcaacc aggtaaggta aaacgttatg catgtgctta
     5341 acattctctg ggtggtattc ggcattggtc tgatgctggt actgaatttg aagttcaaaa
     5401 tcaattcaat ggtggctttg ttggtggcgg cgctgtccgt cgggatgctg gcgggcatgg
     5461 atttgatgtc gctgctgcac accatgaaag cgggcttcgg caacacgctg ggggaactgg
     5521 ctatcatcgt ggtgttcggt gcggtcatcg gtaaattgat ggtcgactcc ggcgcggctc
     5581 accagatagc gcatacgctg ctggcgcgtc tcggtctgcg ctatgtacag ctgtcggtga
     5641 ttatcatcgg cttgattttt ggtctggcga tgttctatga agtggccttt atcatgttag
     5701 cgccgctggt tattgttatt gccgccgaag ctaaaattcc gttcctgaaa ctggcgatcc
     5761 cggcagtagc agctgccact accgcacatt cactgttccc accgcagccg ggtccggtgg
     5821 cgctggtgaa tgcttatggc gcggatatgg ggatggttta tatctatggc gtactggtga
     5881 cgatcccaag tgtaatctgc gcaggtctga tcctgccgaa gttcctcggc aatcttgagc
     5941 gcccaacgcc atcattcctg aaagcagatc aaccggtaga tatgaataat ctgccctctt
     6001 tcggcgtttc gattctggtg ccgctgatcc cggcgatcat tatgatctct accaccatcg
     6061 ccaatatctg gctggtaaaa gatacccctg cctgggaagt ggttaacttt atcggttcct
     6121 cgccgattgc aatgtttatt gcgatggtgg ttgcattcgt actctttggc accgcacgtg
     6181 gtcatgacat gcagtgggtg atgaacgctt ttgaaagcgc ggtgaagagt attgcaatgg
     6241 tgattctgat catcggtgcg ggtggcgtgc tgaagcagac tatcatcgac accggcattg
     6301 gcgacaccat cggcatgttg atgtcccacg gcaatatctc gccctacatc atggcatggc
     6361 tgatcactgt gctaattcgt ctggcgacgg gtcagggtgt cgtttcggcg atgaccgccg
     6421 ccgggattat aagtgctgca atccttgatc cagcaactgg tcagctggtt ggcgtgaatc
     6481 cggcgctgct ggtactggcg acggctgcgg gttccaacac cctcacccac attaacgatg
     6541 catctttctg gttgttcaaa ggttactttg acctgtcagt aaaagacacg ttgaaaacct
     6601 ggggtctgct ggagctggtc aactccgtgg ttgggctgat tattgtgctg attattagca
     6661 tggtagcgta aaaggacacc agagcctgcc aatggcaggc tctgactgat gacaaagtct
     6721 aaaaatgtgc ccagacagtc ctctcgcccc tctggggaga gggttagggt gaggggaaaa
     6781 gaccagcaaa gtgcgctttg tttatgccag atgcgacgct aacgcgtctt atctggccta
     6841 caaagggcta acgtgcaggt ttttagcttc aggtaatatt gcgtacctgc attagcaatg
     6901 ccctgtgatt tctttattga taaacaaaag tcacgccaat aatcgattgc acattccctg
     6961 cagtcacctg ccctccggta cgtgcgtaat ttgccgttaa tcccagactt accgccgaag
     7021 tccctactgc tcctaacgat accgtgttat tcgctggaat aatcgtaccg ttgcgcgtca
     7081 actgtacgcc gacgccctgt gcaggtgaaa acgacgcggt attggtgaaa atcgagttgc
     7141 ccgcatctgc ggttgtgccg gagaggtaat accccaggtt ttggcttttc gcacaataaa
     7201 cggtaagagg aattggcact gaaccagggt agtccggcag agtaacggtg acatcacgag
     7261 cagaaacatc gcagccgcca gtaggcacca ccacatcatt attggcgtaa atattccaca
     7321 caaactggaa atcatcgctg ttatagttgt tggtctgtcg caaaattagc acggcaatta
     7381 atgagccagc tttaatcgcc accccgcccg cactgctcac aggcgtcaaa taaagcgcca
     7441 ccggccacgg cttatccgtt ctcgaattat aaacaacgcg cggcgtttcg ctggtggtag
     7501 gaaatggata gctactgcca ctatatttta cggtcccgga aaaattagat aacacgccgc
     7561 cataagccga gcctcgttgc agtgtgacat agtctgtaat ggtttccgga taatcgttat
     7621 ggcaaaagat ttgcgtcgaa agatccacga ccaggttttg ccccacattc acggcgggcg
     7681 caaggtttac ataaacattg gcgctgccac cgccaatagg gatagcggta ccattggcgg
     7741 ttttacaggc gaatgaccag gcatttaccg accagcccat cagcagtaca gcaaacaggg
     7801 taataactcg tttcattaca atcatctctt cgggttcagc tgtaggtata ggtgatgcta
     7861 atcactgcct gaatggttcc ctgagtggct ccgccattta ccgtcaatgc tctgacctgt
     7921 aacgggaagt gcgctgattg tgaggaatca tccacctgaa ctgttttggt tgcgccagta
     7981 ttcaatgtgt tgccactgtc atcctgtagc tctaactgga tgttttgcgc ggtcccctgg
     8041 tttttataat atccggtact gtcggctgcc ccgctgaagc tggcagtgac cctcgacgtt
     8101 cccaccggac aattagtcaa ctcaagcgca acatcatgcc aggccgatgc cgccccggca
     8161 gacatcagac tgaaagaata aagatcgccg agatcaaccg tggcattggt ggtggaaact
     8221 gtgcacggtt tggcgacgac cttaccgttc accgtgatgg tgacatcggc tgcctgtatc
     8281 gtcgcacttg cgagcgccaa tattgccgcc aatacatacc cacgtttgca ccatttcatg
     8341 agcatctcca gttactgata ttcaagagtg aaggtagccg tggcattgat atgccctgca
     8401 gtgacaggca cctgtgtcgc cattagccgg gcgtaaaaat tcagcgtatt tggtttaccc
     8461 ggcgtcaggg tcgtccacga aagcgcggac gatggagcat taaggggtat ttgattttgc
     8521 tgctcattca gaagctgtat tcccagtccc gaagccgctg acaccgtatt ttcaagtgca
     8581 agcagattgg cattgtggct atctgcaacg ccggtaaacc caacttttac ggcagaaacg
     8641 gcattaccac agggtgacag caaaatacaa aatggaacaa caggagtcgt cgcgccaatg
     8701 ttgttaaatt gcttcgccgc gttttccatc agatcaacag taaaattggt tgattcagcg
     8761 gccacactac agccgttatc cctgacatag ccgcggatag taatcgtgct atccgcagcc
     8821 aaagcgtgac tcaccgccag ccacaaaaaa gcgcacagaa gataaaaagg tttgtttctc
     8881 atcacgcccc cttaacgaca ttcagctgat agctgggtta ataactgctg ctgactctct
     8941 ggtggcagtt gataattggc gacacagtga gcattttcct cttctcccca tttcacctga
     9001 acttttcccg ctaaaggcat tccgctgagg taaacctgac cattatccgc aacaatgccg
     9061 ctactctggc tactctctga tgtcaccatc gccccaaacg gcagcggctt attattgtgg
     9121 gtcagcgtca tgagcagttt tatcccaacg cgcgctttaa actctgctcg cacgatcgcc
     9181 ccacgagtgg gaacaacgtt agcaaccgcg ttatctaaat cgacgttatc agccagggta
     9241 ttggtatcca gcgccactct attttcccga tattcagtgg cataagctac cactcaaata
     9301 cagtgttgat gtgcgcccga gttgctgagt aacggtgagc tgtaatttcc cgcgtttgtt
     9361 ataagcgagg ttgtaatagt cggtgaattt cggcttaacc tgaataactc cgtcctgtgt
     9421 ttcgatgttg tagccattca ttcgactgta tgttgtatca gcgaaattaa aatatccgct
     9481 ggtcgaataa cggtaaccca ctaactgaat attcgtgcct gactcattga gcgatttgtt
     9541 atagagaaaa cgcaccgatt gtccgtcatg ctgactgtca tcgggaagtg tggaattagc
     9601 ctgcgtcata tccacagaca gagcgcccag tgcccccatg tttttcccga taccgaaatt
     9661 aaaagcacga taacgatccg ccagttgcgt tccaccatat attgtccagc cagccggaag
     9721 gccgtggagt aatgtactct ggaaaaagcg gggtttttcc tgctgcgcat ttccactacg
     9781 gtattctcct gccgtaatgg aataacgagt atgcccttca cgttgcaaaa gcgggactga
     9841 cgaatagggt acggtaaaaa tctgcgtgct gccgtcagcc tctttgatcg ttacctgcaa
     9901 gtcaccacta ttacctgcgg catagatatc gttgatggta aaaggccccg gtggcaccgt
     9961 actattataa atgtcatacc cattttgttt aatagtgacc tgtgcagtac cacgagcaat
    10021 accgtggatc accggggcaa atcctctttg gctatcgggt aacatattgt catctgaggc
    10081 caattgtgcg ccgcgaaagt taataccatc gaaaatatcg ccctgagtat aaccatcacc
    10141 cagcgtcagc cgggaacgta acggtattat gtctcgctca agccaggtat tgatatgctg
    10201 ccatttattt ttgctacctg atgatctgtc gctacggtta taactccagg tggtattgtc
    10261 gcgtaaacgc cacgcaccaa tatttaaccc actctgtagg tttaaatatg cataatggct
    10321 gttaccccca atccgattct gtacactatt tccgctgaaa ttataattga gcaatccggc
    10381 attaataccg ggatcccata actcaggagg aatataacca cgcgcgcgat tactcataaa
    10441 tgcctgaggg atcgtcaggt tgagtcgctg ctgaccaaca tctaaatgcg cagtagcgtc
    10501 atggattatt gaggttaatg gcacgcaggc atcatccgcc agcagattca taccggagac
    10561 agaagccgta ttcagcccca tactggcaag ttgcgcgcgt gtcaggcagg gaacaatccc
    10621 ttgttcgctg tcgcccgtat taaatgtgac atcacgcgtt gccatataac cattattcaa
    10681 atagatatcg acacgatacg tccctggcgg taattcttgc ccattttcaa aacgcgataa
    10741 atcagccaca gcctggggat catccgctaa aaagcgcgga ttaaaataga gttcggcaga
    10801 tgacaaaggt gtctgtgcgg caaaagcaca ggcgacaaag agccggacaa aaaaaccagc
    10861 caaacgatgc ttacgaatat gcaagcattg tgtgtttcgc tggtaaagtc ttaaattcag
    10921 atatgacatt attcctccgg tttaaaatca cccggcagtc aattcttttt attcaggcga
    10981 aaaattcccc ctgcgttatt ccattacgcc cgtcattttg ggggtaagtg cgccataatc
    11041 atttattgtt cggtaagtaa tattgcttcc tgcatcagaa ggcaatttaa ccgtgctttc
    11101 gcccattgga ggcaccaatg cattttcaag aacccgggtt ccggcattca actctgttac
    11161 cgtcaggtaa tagggtgtcg ggttaatcag cgtcagggaa ttcgcgctac gacgaaatct
    11221 taatttttct gcggcctgat cgggtggcaa cgctaattta gccggacgat agtacagttt
    11281 aatgcggctg ataattgcga gctgtagcgt attctcagtc aattttgatt tatccattga
    11341 cggaatcgct ttaacattca tccagaataa actttcccga tcctgtggca attggttatt
    11401 agttgcatca agaatacgca aggtattctc tctttttccc ttcatcgcaa acagaggagg
    11461 cgtcacgata aaacgaccat cctttacacc atcggcattt tccacccatg attgaattaa
    11521 ataggtacta ttttcatcat tatttgtcac ggcaagttgc acttgttttt gccctgccgg
    11581 ataaattacg cgagtcgcac ctaaggccac tcccgcttca gcgcgtccgg caaccatcat
    11641 tgccatgaac atcaggatac ctgccagcaa gcagaatgtt atttcctgcg atttccttac
    11701 attgacgttt ttattactca ctgtcctgtt cctgttatca cattatctgc tgaacaatta
    11761 ctgataggtt aaagagaacc aggcctgtgc attggcgatg ccgccagtaa cccgacgccc
    11821 ggtagcacga tatttggcga tgaaatgtag cgaagtagag cctgagtaaa gccgtttcca
    11881 gtttgctgga ggacgattaa tcggtacgag gtttccttca tcatcaaaca acgctacgcc
    11941 aatattggtg gctatccctg gcccctctcc aacggaaagc acatctggat ttttaccatc
    12001 cgcgacaccg tgaaacgcca cacccacatg ttcactcacc accgtgctac attcccgtaa
    12061 atgaataaca aaaggcaccg gtgcgctatc ttccccaacc gcatgaaacc ggttactgct
    12121 gatttgcccc atattgaccg tcatttgttt atcaccggct tcaatccggc aagtttccgc
    12181 aataatgacg ccctgaaatt gcatatttcc gccgggcaac gtggtattcc atttatttcc
    12241 ggccagagca aacattggca gcaacgacgc taatagaaat aatctcttcc ttttcatcgt
    12301 tatttttatc gcacaagggt gggcatccct gcccgtaatg acgtccctga acctgggtag
    12361 gttattgata ctgaaccttg aaggtcgcat ccgcattagc tgcgccaggg gttgcggcac
    12421 cggttgcaaa ataacgcgcc tggaacggaa tggtgttggt tccgttattc agggttgttt
    12481 ctgaactaaa tgtcgcaccg tccagcgcca gcgcagcacc cgttctgtcc aggatctgca
    12541 caccaacgtt tgttgcgcta cccgcagctg aactctgcag agccagtaca tcagtatgcg
    12601 tagcatcaat tgccgtacct aagaaggcaa cagcggcttt tgtggcaaca gtggtatcgc
    12661 aatcattcag ctgaatgtta aaaccaacgg cagagctggt tgctccagcc tgcttcaggc
    12721 tagcggtacg aacctggccc aactgaacgg tttgatcaac agagcctgca tcaactgcgc
    12781 aagcggcgtt aacaacttcc cctttaaagt gaacggtccc accatttacc gtcgtagtat
    12841 cggccagagc cgctgcggaa ctgagggaca gagccgacag aacaacgatt gccagagttt
    12901 taattttcat gctgctttcc tttaaaaaaa ctatttctaa atcgacatgg gcagtcgttc
    12961 tgtacacttt gttttgtcaa cgagtttgac tgccaacact gcacagtttt cccccaaaag
    13021 atgaaacatt tggggccatt ttgactcata gaggaaagca tcgcgcacaa actttttcag
    13081 tttatttgcc ggcttaatat tctattgtta tctttattta tagattttta tattgcatga
    13141 ggtggttttt ggagagaaga atgaggaaga tgcgtcgagc cac
//
LOCUS       DAZFQD010000145        13029 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.145, whole genome shotgun sequence.
ACCESSION   DAZFQD010000145 DAZFQD010000000
VERSION     DAZFQD010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13029
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.145"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            271..837
                     /gene="yqaB"
                     /locus_tag="ACHKTW_004226"
     CDS             271..837
                     /gene="yqaB"
                     /locus_tag="ACHKTW_004226"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HFO6465145.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGHYGLQYDVQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
     gene            834..1261
                     /locus_tag="ACHKTW_004227"
                     /pseudo
     CDS             834..1261
                     /locus_tag="ACHKTW_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
     gene            1334..2890
                     /gene="gshA"
                     /locus_tag="ACHKTW_004228"
     CDS             1334..2890
                     /gene="gshA"
                     /locus_tag="ACHKTW_004228"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HFO6465146.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLLQVGKDLFRDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMEAADTE
                     PFAVWLEKHA"
     gene            3040..3555
                     /gene="luxS"
                     /locus_tag="ACHKTW_004229"
     CDS             3040..3555
                     /gene="luxS"
                     /locus_tag="ACHKTW_004229"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFO6465147.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILERDVRINSNEELA
                     LPEEKLQELHI"
     gene            complement(3619..5157)
                     /gene="emrB"
                     /locus_tag="ACHKTW_004230"
     CDS             complement(3619..5157)
                     /gene="emrB"
                     /locus_tag="ACHKTW_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HFO6465148.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDWFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(5174..6346)
                     /gene="emrA"
                     /locus_tag="ACHKTW_004231"
     CDS             complement(5174..6346)
                     /gene="emrA"
                     /locus_tag="ACHKTW_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
                     /protein_id="HFO6465149.1"
                     /translation="MSANAETQTPQQPVKKSGKRKRLLLLLTLLFIIIAVAIGIYWFL
                     VLRHFEETDDAYVAGNQIQIMSQVSGSVTKVWADNTDFVKEGDVLVTLDPTDARQAFE
                     KAKTALASSVRQTHQLMINSKQLQANIEVQKIALAKAQSDYNRRVPLGNANLIGREEL
                     QHARDAVTSAQAQLDVAIQQYNANQAMILGTKLEDQPAVQQAATEVRNAWLALERTRI
                     VSPMTGYVSRRAVQPGAQISPTTPLMAVVPATNMWVDANFKETQIANMRIGQPVTITT
                     DIYGDDVKYTGKVVGLDMGTGSAFSLLPAQNATGNWIKVVQRLPVRIELDQKQLEQYP
                     LRIGLSTLVSVNTTNRDGQVLANKVRSTPVAVSTAREISLAPVNKLIDDIVKANAG"
     gene            complement(6473..7003)
                     /gene="emrR"
                     /locus_tag="ACHKTW_004232"
     CDS             complement(6473..7003)
                     /gene="emrR"
                     /locus_tag="ACHKTW_004232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HFO6465150.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRESDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            complement(7094..7429)
                     /gene="ygaH"
                     /locus_tag="ACHKTW_004233"
     CDS             complement(7094..7429)
                     /gene="ygaH"
                     /locus_tag="ACHKTW_004233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HFO6465151.1"
                     /translation="MSYEVLLLGLLVGVANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            complement(7419..8156)
                     /gene="ygaZ"
                     /locus_tag="ACHKTW_004234"
     CDS             complement(7419..8156)
                     /gene="ygaZ"
                     /locus_tag="ACHKTW_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HFO6465152.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALTVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            complement(8280..9464)
                     /locus_tag="ACHKTW_004235"
     CDS             complement(8280..9464)
                     /locus_tag="ACHKTW_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO6465153.1"
                     /translation="MTKPNHELSPALIVLMSIATGLAVASNYYAQPLLDTIARNFSLS
                     ASSAGFIVTAAQLGYAAGLLFLVPLGDMFERRRLIVSMTLLAAGGMLITASSQSLAMM
                     ILGTALTGLFSVVAQILVPLAATLASPDKRGKVVGTIMSGLLLGILLARTVAGLLANL
                     GGWRTVFWVASVLMALMALALWRGLPQMKSETHLNYPQLLGSVFSMFISDKILRTRAL
                     LGCLTFANFSILWTSMAFLLAAPPFNYSDGVIGLFGLAGAAGALGARPAGGFADKGKS
                     HHTTTFGLLLLLLSWLAIWFGHTSVLALIIGILVLDLTVQGVHITNQTVIYRIHPDAR
                     NRLTAGYMTSYFIGGAAGSLISASAWQHGGWAGVCLAGATIALVNLLVWWRGFHRQEA
                     AN"
     gene            complement(9755..10747)
                     /gene="proX"
                     /locus_tag="ACHKTW_004236"
     CDS             complement(9755..10747)
                     /gene="proX"
                     /locus_tag="ACHKTW_004236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HFO6465154.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWTEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            complement(10805..11869)
                     /gene="proW"
                     /locus_tag="ACHKTW_004237"
     CDS             complement(10805..11869)
                     /gene="proW"
                     /locus_tag="ACHKTW_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HFO6465155.1"
                     /translation="MADQNNPWDTTPAADSAVQSADAWGTPATAPTDGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALIAWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            complement(11862..13028)
                     /gene="proV"
                     /locus_tag="ACHKTW_004238"
     CDS             complement(11862..13028)
                     /gene="proV"
                     /locus_tag="ACHKTW_004238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016242336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0031460 - glycine betaine transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HFO6465156.1"
                     /translation="MFGEHPQRAFKYIEQGLSKEQILEKTGLSLGVKDASLAIEEGEI
                     FVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAELREVRRKKIAMVFQS
                     FALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHSYPDELSGGMRQR
                     VGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTIVFISHDLDEAMR
                     IGNRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSAKDIARRTPNGLI
                     RKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALTQQQGLDAALIDA
                     PLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRALDREGVNNG"
BASE COUNT         3225 a         3348 c         3464 g         2992 t
ORIGIN      
        1 aaccttcagc aatgaaggta tcgaagagat agcggtatta accgcaggca ccagggagga
       61 taacgttgcg tcagcaacgg cccgaagggc gaacgccagt gagtaatcct cccggatgca
      121 ccatcttctc ccaaaaattt ccttcaaaaa tatctcattt ctaaataact actttatcca
      181 tttcctgaat tttcttctcc ttcgccatca gcgacaaaat caagcaatta cgctaaagta
      241 gcgtcatatt tttcgacttg cgagaagacc atgtacgagc gttatgcagg tttaattttt
      301 gatatggatg gcacaatcct ggatacggag cctacgcacc gtaaagcgtg gcgcgaagta
      361 ttagggcact acggtcttca gtacgatgtt caggcaatga ttgcgcttaa tggatcgccc
      421 acctggcgta ttgctcaggc aattattgag ctgaatcagg ccgatctcga cccgcatgcg
      481 ttagcgcgtg aaaaaacaga agcagtaaga agtatgctgc tggatagtgt cgagccgctt
      541 cctcttgttg aagtggtgaa aagttggcat ggtcgtcgcc caatggctgt aggaacgggg
      601 agtgaaagcg ccatcgctga ggcattgctg gcgcacctgg gattacgccg ttattttgac
      661 gccgtcgtcg ctgctgatca cgtcaaacac cataaacccg cgccagacac atttttgttg
      721 tgcgcgcagc gtatgggcgt gcaaccgacg cagtgtgtgg tctttgaaga tgccgatttc
      781 ggtattcagg cggcccgtgc agcaggcatg gacgccgtgg atgttcgctt gctgtgagtg
      841 aagcgttatc gcttttctcg ttgtttgcca gtagttttct cagcgctaca ctattacccg
      901 gcaactccga agtcgtgctg gtggcaatgt tgctttccgg gatcagttat ccctgggttt
      961 tagttttaac agcaacaatg ggtaatagcc ttggagggtt aactaacgtt atccttgggc
     1021 gtttctttcc attgcgtaaa acatcgcgct ggcaagagaa agctactggc tggctgaaac
     1081 gctatggtgc agtcacgcta ttattaagct ggatgcccgt ggttggcgat ttactgtgtc
     1141 tgttagcggg atggatgcgc atctcgtggg gaccggtaat ctttttttgt gccttggtaa
     1201 agcgttacgc tatgttgcag ttgcagcagc taccgttcag ggcatgatgt ggtggcacta
     1261 attgtaggcc tgcacatatg gtcaccatta cagttatgct aattaaaacg attttgacag
     1321 gcgggaggtc aatttgatcc cggacgtatc acaggcgctg gcctggctgg aaaaacatcc
     1381 tcaggcgtta aaggggatac agcgtgggct ggagcgcgaa actttgcgtg ttaatgctga
     1441 tggcacactg gcaacaacag gtcatcctga agcattaggt tccgcactga cgcacaaatg
     1501 gattactacc gattttgcgg aagcattgct ggaattcatt acaccagtgg atggtgatat
     1561 tgaacatatg ttgaccttta tgcgcgatct gcatcgttat acggcgcgca atatgggcga
     1621 tgagcggatg tggccgttaa gtatgccatg ctacatcgca gaaggtcagg acatcgaact
     1681 ggcacagtac ggcacttcta acaccggacg ctttaaaacg ctgtatcgtg aagggctgaa
     1741 aaatcgctac ggcgcgctga tgcaaaccat ttccggcgtg cactacaatt tctctttgcc
     1801 aatggcattc tggcaagcga agtgcggtga tatctcgggc gctgatgcca aagagaaaat
     1861 ttctgcgggc tatttccgcg ttatccgcaa ttactatcgt ttcggttggg tcattcctta
     1921 tctgtttggt gcatctccgg cgatttgttc ttctttcctg caaggaaaac caacgtcgct
     1981 gccgtttgag aaaaccgagt gcggtatgta ttacctgccg tatgcgacct ctcttcgttt
     2041 gagcgatctc ggctatacca ataaatcgca aagcaatctt ggtattacct tcaacgatct
     2101 ttacgagtac gtagcgggcc ttaaacaggc aatcaaaacg ccatcggaag agtacgcgaa
     2161 gattggtatt gagaaagacg gtaagaggct gcaaatcaat agcaacgtgc tgcagattga
     2221 aaacgaactg tatgcgccga ttcgtccaaa acgcgttacc cgcagcggcg agtcaccttc
     2281 tgatgcgctg ttacgtggcg gcattgaata tattgaagtg cgttcgctgg acatcaaccc
     2341 gttctcgccg attggtgtag atgaacagca ggtgcgattc ctcgacctgt ttatggtctg
     2401 gtgtgcgctg gctgatgcac cggaaatgag cagtagcgaa cttgcctgta cgcgcgttaa
     2461 ctggaaccgg gtgatccttg aaggtcgcaa accgggcctg acgctgggta tcggctgcga
     2521 aaccgcgcag ttcccgttac tgcaggtggg taaagatctg ttccgcgatc tgaaacgcgt
     2581 cgcgcaaacg ctggatagca ttaacggcgg cgaagcgtat cagaaagtgt gtgatgaact
     2641 ggttgcctgc ttcgataatc ccgatctgac tttctctgcc cgtatcttaa ggtctatgat
     2701 tgatactggt attggcggaa caggcaaagc gtttgctgaa gcgtaccgta atctgctgcg
     2761 tgaagagccg ctggaaattc tgcgcgaaga ggattttgta gccgagcgcg aggcatctga
     2821 acgccgtcag caggaaatgg aagccgcaga taccgaaccg tttgcggtgt ggctggaaaa
     2881 acacgcctga cagaaaagaa aaaggccact cgtgagtggc caaaatttca tctctgaatt
     2941 cagggatgat gataacaaat gcgcgtcttt catatactca gactcgcctg ggaagaaaga
     3001 gttcagaaaa tttttaaaaa aattaccgga ggtggctaaa tgccgttgtt agatagcttc
     3061 acagtcgatc atacccggat ggaagcgcct gcagttcggg tggcgaaaac aatgaacacc
     3121 ccgcatggcg acgcaatcac cgtgttcgat ctgcgcttct gcgtgccgaa caaagaagtg
     3181 atgccagaaa gagggatcca taccctggag cacctgtttg ctggttttat gcgtaaccat
     3241 cttaacggta atggtgtaga gattatcgat atctcgccaa tgggctgccg caccggtttt
     3301 tatatgagtc tgattggtac gccagatgag cagcgtgttg ctgatgcctg gaaagcggca
     3361 atggaagacg tgctgaaagt gcaggatcag aatcagatcc cggaactgaa cgtctaccag
     3421 tgtggcactt accagatgca ctcgttgcag gaagcgcagg atattgcgcg tagcattctg
     3481 gaacgtgacg tgcgcatcaa cagcaacgaa gaactggcgc tgccggaaga gaagttgcag
     3541 gaactgcaca tttagtcagt aaactatctt cacaattaat tgaaaaaagc cagttcaaat
     3601 gaactggctt agttgtactt agtgcgcacc gcctccgccg ccacctgcgc caaatggcgg
     3661 tttagcaaac cacaccagcc ccagcaggac gaggaatatc ccggctgaca tccagaagat
     3721 ctcattggcg gaaataatca gcccctggtt ggtgatctgc tgggcaatcc agccggaggc
     3781 ctgctgttgc gtcatcccaa gcccttccag ttgactgtac atcgcctggg cgtttgggtt
     3841 aaacgggttt accgactcgg ttaactgtgc gtggtgcatc gactcgcggt tggtccacat
     3901 ggtggtggtg atggacgtgc cgatagaccc cgccagcgta cgcgtaaagt tggagaggct
     3961 cgatgccgcc gccagtcgtt ccggtggcaa accagacagc gtaatggtgg tcagcggcat
     4021 aaagaagcag gccaccgcaa acccctggat aaactgcggc caggccgacg cgccaaaatc
     4081 catacctggt tcaaaggtat aggcacgcca gtagaagcag acggcataca taataaagct
     4141 gaaggttacc agccgccgca tatccagttt atgcgcgaag cggccgataa tcggcgacag
     4201 gatcaccgga ataatcccta ccggcgcaga ggccaaacct gcccaggtcg ccgtgtaacc
     4261 gtagacctcc tgcaacaact gcggcagcag aacaatagcg ccgaagtaga gcatatacgc
     4321 gaggctgata cacaagcagc cgatggtgaa gttgcgcgac ttaaacaacg acagatcgac
     4381 tatcgggtta tcgtcggtca gctcccagac aatcaggaag cagatagcca ccaccgccac
     4441 cacggtaagg atgataattt cctgtgatga aaaccagtcc agctctttac cgcggtcgag
     4501 cataatctgc aggctgccga taccaataac cagcagtgcc agccccacgg catcaatccg
     4561 ccgccgttcg gtgcgggttt cacgtccgcg cagagtttgc agtgtcatca acaccaccgc
     4621 cacgccaatc ggcacgttga tgaagaatat ccagccccag tggtaattat cgctgatata
     4681 accgccgagg atcgggccgc aaattggcgc gacaatcacc gtcatcgacc acaacgccag
     4741 cgcgatcgag cgtttggctg gcgggtagtt attcagcaat agactttgcg aaagcgggat
     4801 caacggcccg gcgacaatcc cctgaatcac gcggaagaag atcagcatat tcaggctgct
     4861 ggagacacca cacgcccacg acgcaatagc aaaggcgatg gtggaccaaa ggaacagttt
     4921 cacttccccg acgcgctttg ccagccagcc ggtaagcggg atcgagatgg cattcgccac
     4981 cccgaaagaa gtgattaccc acgttccctg gctgagcgat gagcccagat tcccggcgat
     5041 agtggggatc gccacgttag caatggtgga gtccagcacc tgcatgaatg tcgccagtga
     5101 cagcgcaatc gtcataatga ccagttgcgc gccttccagc ggtttttgct gttgcatcac
     5161 acgcacctct ggattagcca gcgttagctt ttacgatatc gtcgatcagt ttattgacag
     5221 gtgccaggct gatttcacgc gcggtgctta ccgctaccgg agtggaacgt actttatttg
     5281 ccagtacctg accgtcacgg ttagtggtat tgacgctcac cagcgtggac aaaccgatac
     5341 gcagcggata ttgctccagc tgtttctggt ccagttcgat acgcacaggc agacgctgaa
     5401 cgactttgat ccagttaccg gtcgcatttt gcgctggaag cagtgagaac gcgctacctg
     5461 tgcccatatc cagaccaacc actttaccgg tgtatttcac atcatcgccg taaatatccg
     5521 tggtgatagt gaccggctga ccgatacgca tattggcaat ctgcgtctct ttaaagttgg
     5581 catccaccca catattggtg gctggaacga ccgccatcag cggcgtcgtt gggctaattt
     5641 gcgccccagg ctgtaccgcg cggcgggaga cataaccggt catcggactg acaatacgag
     5701 tacgctccag cgccagccag gcgttacgta cttcggtggc agcctgttgc acggctggct
     5761 gatcttccag tttagtcccc agaatcatcg cctgattggc attgtattgt tgaatcgcga
     5821 cgtccagttg cgcctgggca ctggtgacgg cgtcgcgggc gtgttgcagc tcttcgcgac
     5881 caatcaggtt ggcattgccc agcggcacac ggcggttgta gtcgctttgt gctttcgcga
     5941 gggcgatttt ctgcacctca atattcgcct gcaactgctt gctgttaatc atcagctggt
     6001 gggtttggcg aacgctggaa gccagtgcag ttttggcttt ttcaaacgcc tggcgagcat
     6061 ctgtcgggtc gagagtgacc agcacgtcgc cttcttttac aaaatcggtg ttatcggccc
     6121 agactttcgt cacgctgcca gacacctgag acataatttg aatttgattc cctgccacgt
     6181 atgcgtcatc ggtttcttcg aaatgacgca gtaccaaaaa ccaataaatc cctatcgcta
     6241 cggcaataat tataaagagc aaggtgagaa ggaggagcag acgcttacgt ttgccgctct
     6301 tctttaccgg ttgctgcggg gtttgagtct ccgcatttgc gctcatattg ttctccacga
     6361 tcttcttaac tcatcggctg agccgacctg cttgccaaaa aggccagcat gttgcgatgc
     6421 tggccggtca ttttttcttt tataaatctg gatttttgag cgagatgacg cgttagctca
     6481 tcgcttcgag aaccacaccg tcttgttcca tctggtcgag acgggagagc aatttgcggg
     6541 tgatttgctc gagctgatct ttttctgttg tgctgagcgc ggaccagagt tgatgcaggc
     6601 agttatgctg cggtggtaaa acctcgcgca aaaactcgtg acctttttcc gttaattgca
     6661 gatgcaggca gcggcgatcg ttatcgcttt cacgacgttc gatccaaccg cgtttttcca
     6721 gttcatcggc aatacgcgtc gcgttggtac gggatgatcc aagagcacaa cttaattcag
     6781 aaggctgaat actgtggttt tcctgagact ccagcgtaat caacgccata aacaacgtct
     6841 cgttaatccc ctgagccttc agcattttat tgcggttctc cagcagcttg ctttgcatgt
     6901 gcatgcaaag acgagtcaga aggatctcct gataaggaaa atcttcgtgg cggctggcgc
     6961 gaaattttag catttgttca atgggcgtaa acgaactatc catttgggta tgacctcatt
     7021 aatttcatcc gatatagtaa cgacagtgac aaataaagta aatgtattgt tttagaaaaa
     7081 tgattcttgt gggttatata atcgccatca ctttccaggc gagcccatag gccagcgcac
     7141 taagcagtgt tgggatgata atgctgcgtg ttttatagaa actggcaccc agtaccgcga
     7201 agccgaccag cgtgggcacg aaacggcgtg tatcgtgcat cacttctggt gcggtagaga
     7261 caaccagcag agcgcatatc gaggcgatgc caatggtgtc gagcaaaata cctaccgcgc
     7321 cacgtttggt tgggcgggca ttacccacac gcaggcgcag cggcaaatag cggaagcaat
     7381 aattcgccac gccaaccagt aacccaagca gcagaacctc atagctcatc gggcgctcct
     7441 tgccagaatg cctggattaa cgcagtgagg cagccacaga caatgcctgc cagaatggcg
     7501 acgggaatag aaaatagcgt tacgcctgca agggcaccaa ctaacgctgc ggtaacgcaa
     7561 agagattgtt tgcgctggaa agaggcgagc aggaaactca taaagagtgc cggaagcata
     7621 aaacctaatg cagcttcaac ggcgggataa ccttgcagca agccgctgcc ggagaatgcc
     7681 cctattaccg taccaaatac ccacgatgac catgaactga aggcaatgcc gatcatccag
     7741 ttctcgctcc agcggcgatt attgcgtacc agttttgcgg ttgcggcggc aaaaacctca
     7801 tccgtcaggc caaatgccca cagggcggtt ttcgattttt gcagacgctg aataatacgg
     7861 ctacgcagtg acgggccata caacacatgg cgaacatcca ttgccatgac ggtcagtgca
     7921 gcaacccaca aactactccc ggctgccagc atcgcggtaa tgacgaactg gctcgcgcct
     7981 gcataaatga tgcaggagaa aaaaacgctt tcgagaggag agaatcccag acgggtcgca
     8041 ttcagaccga acgcaaaggc caccggaata taactaataa caatcggtaa actgtctttg
     8101 catccttcca tgaaggtcgc cgaaccagga gcaggctgtg gagtagggct ttccatagag
     8161 tgtacgctta acgattgtta atattataaa tagactgaat gaatatctta accttatcag
     8221 actgatgggc ttcttaacac ccttataagt gtaaagccac gaaaacggtt gctgattgct
     8281 taatttgcgg cttcctgacg atgaaaacct cgccaccaga ccagtaagtt aaccagggca
     8341 atcgtcgcgc cagccagaca aacgccagcc caaccgccat gttgccaggc tgaggctgaa
     8401 attagcgaac cggcggcacc gccaataaag tagctggtca tgtaacctgc ggtcaggcga
     8461 ttacgcgcat cagggtgtat tcgataaatt accgtctggt tagtgatatg cacgccctgt
     8521 acggtgagat ccagcaccag gatgccgata atcagcgcca gtacggaagt gtgaccaaac
     8581 cagatcgcca gccatgaaag taatagcagc agcagaccga aagttgtggt gtggtgcgat
     8641 ttacccttat cggcaaaacc gcccgccgga cgagcgccca acgctccggc agctcccgca
     8701 agtccaaaca gaccaatgac accatcgctg tagttaaaag gtggagcggc aagcaaaaag
     8761 gccattgagg tccagagaat gctgaagttg gcaaaggtca ggcagcccag caatgcgcgg
     8821 gtgcgcagga tcttatcgct gataaacatg ctgaaaacgg aacctaacaa ctgtgggtag
     8881 ttgaggtggg tttctgattt catttgtggc agaccacgcc ataatgccag cgccatcagt
     8941 gccattaaca ccgaggcaac ccaaaagacg gtgcgccagc cgccgagatt cgccagcaat
     9001 ccggcaacgg tccgtgcgag taagatcccc aacagcagac cgctcataat agtgccaacc
     9061 actttgccgc gtttgtccgg tgaagccagc gtcgctgcca gcggaaccag aatttgtgcc
     9121 acgacagaga ataaaccggt taatgccgta ccgaggatca tcatcgccag tgactgactg
     9181 ctggcagtga ttaacatacc gcccgctgcc agtaaggtca tcgagacaat caggcggcgg
     9241 cgttcaaaca tatcaccgag gggaacaaga aacagcagac ctgctgcata gcccaattgc
     9301 gcggcggtaa caataaagcc tgccgaactg gcggaaaggg aaaagttacg cgcgatggtg
     9361 tcgagcaatg gctgggcgta atagttactg gctaccgcca gaccggtggc gatagacatc
     9421 agcacgatca gcgccgggct aagctcatga ttaggtttag tcattatttt tgttcatcag
     9481 gtgatgatta agctggaaat caataatagc ggatgttgat gaatatggga tgaaagatag
     9541 attgttgaaa tgtgcgatgt ggtttgtagg catgataaga cgcgccagcg tcgcatcagg
     9601 cattgtgcac agaacggcgg atgcggcgaa aacgccttat ccgccatacg aatgagtgta
     9661 ttttgtaggc atgataagac gcgtcagcgt cgcatcaggc attgtgtaca gagcggcgga
     9721 tgaggcaaaa acgccttatc cgcctgaata aaaattactt ctgcgctgcc agcgcctcat
     9781 tcacccagcc atcgaactgc tgctggtggg ctttgatcca gccatcaacg tggccctgaa
     9841 tatcgccttc tgaggctttg ccgtcatgca taatggcgtt ctgggcgtta atatcggcca
     9901 ctggcaactg cataatggca aacagtttgg ctgccgccgg atttttctcg gtccaggctt
     9961 tgttggcaac gatatgcatg gtgctgaccg ggaagccgta attcgcgcca ttcggcagtt
    10021 tggtatcggc atttttatcg cccggcagtg cggagaacgg cacctgcaac cagaccacat
    10081 ctttccctgg cttcagctca ttactcaccc agtacggcgt ccaggtgtaa taaaacaccg
    10141 gtttgccctc tttgtagcga ctgatggtgt cggccatcat cgctgcgtag ttcccctgat
    10201 tatgcgtcac ggtgttggtc agttcatacg cggcaagctg gtggttgatg gcaccttcgc
    10261 agccccagcc agggttacaa ccggttaaat ccgctttacc gtcgccgttg gtatcgaaca
    10321 gtttggcgat cttcggatct ttcagttgtg cgatgttggt gattttatac tggtcggcgg
    10381 ttttcttatc gatcaggtaa ccctgtgccg cgccgttaac aaatacccct tcacgataaa
    10441 atttcttatc gccaccggca gcttcgtaca tgttgtcatg cagtggcgtc cagttcacgg
    10501 cggtgaaggt tgcatcgccg gaagcaagcg aggtgtagcc aacgttgtaa tctacttcgc
    10561 tgggtttgtt gacggtatag cctaatttct ccagcgcacg gctgaccagc agcgtctgga
    10621 aggtttcttc ggtgatggtg ctttgaactg gatttacagt aatacctttg cccggcagat
    10681 cggcagcaaa agtttgtgta gagataagcg tggcaaacgc tgtcgcaaaa agtacgctat
    10741 gtcgcattgt tattcctttt tttggcaggg tgataatgcc cggcgtcacc gggcaagtgc
    10801 agagttactt aatgaatggg cgggtcagca gaccaacagg gccagtggtg taccagcgac
    10861 ggttgccgcg actgcgtgag tcgcgcccaa cggcctgcgt caggcgatcg aggataatgg
    10921 cgaggatcac aatcccgacg ccgccaacgg tggcaagccc catatccaga cgaccgatac
    10981 cgcgaagtac catctgaccc agcccgccga cggcaatcat cgaggcaatg accaccatag
    11041 aaagggccag catcagcgtc tggttaacgc ccgccataat ggtcggcatc gccagcggta
    11101 actgaacttt gaacagcatc tggcgcgggc tggcaccgaa tgagcgcgag gcttcaatca
    11161 gatccgccgg aacctggtta atccccagaa tggtcagacg gataatcggc ggcagagcaa
    11221 agatgatcgt caccaccacg cccggcacgt taccgatacc aaatagcatg acgattggca
    11281 ccagataaac aaacgctggc gtggtctgca tggcatcaag cagtggacga ataattttcg
    11341 ccgctcgcgg acttctcgcc agccagatcc ccagcggcaa accaatgacg atacagaaca
    11401 gcagggcggt taacaccagc gccagggtaa ccatggcctg cgaccaggca ccgattgcgc
    11461 cgatggcaat cagcgaaacc agcgtcgcca cgcccattcc gaccccggaa atctgccagg
    11521 cgatgagagc gaaaacgata atcgccaccg gtgcgggcat acccagcagc aattgctgga
    11581 aaccgttgag aatgtaatca accggaacgc gcacgccctg gaagacggga cggaaatggg
    11641 taacaaccca gtcgatccct tcagtgaccc aactgtcgag cgggatcagc gttttatgga
    11701 acggatcgag aatattaaaa tgctcgacgt ttggcgcagg cgtactggtc agccagtcag
    11761 caccaccacc gtcagtcggt gcagtcgccg gtgtacccca ggcgtctgcg gattgtacgg
    11821 cactgtccgc cgctggcgtg gtatcccacg gattattttg atcagccatt atttaccccc
    11881 tcacgatcta aagcgcgcag cagcattcct ttcgaaatga tgccgacata ctgttggtcc
    11941 tcgtcgacca cgggcaccgc acagggtgcc tgtccgacat gagagagcaa ctcgctaaga
    12001 ggcgtttgtg catcgactgc taacggcgca tcaatcagcg ccgcatcaag accttgctgc
    12061 tgcgttaacg cggttttaag cgaatcgatg gaaactgcgc cgacaaactt attaccgcgt
    12121 tcgataacgt agccatattc gcggtcttca tcctgcaata atttcagcgc cgaacgtggg
    12181 ccgaagccag gggttttacg aattaagccg ttcggtgtcc ggcgagcaat atctttcgca
    12241 ctgaatacct gactaatatc gacgccacgg aagaaggtac ggacataatc attcgccgga
    12301 ttattgagaa tttcatccgg tgtgccgacc tgtaccactt caccattttg cataatggca
    12361 attcggttgc caatacgcat ggcttcatca agatcgtggg aaataaagac aatggtgcgc
    12421 tgatgtttcg cctgtaattt taccagctca tcctgcatct cggtgcgaat taatggatcg
    12481 agcgccgaga aggcttcatc cattaataaa atatccggat taatcgctaa tgcacgggct
    12541 aatcccacac gttgacgcat cccgccagag agttcatccg ggtagctgtg ggcataattt
    12601 tccagcccga cctgacgcag tgcatcaagg gctttttccc ggcgttcttc ggcattaatt
    12661 ccggccaatt ccataccgaa cgcagtattg tccagcacgg tcatatgcgg cattaaggca
    12721 aaggactgga agaccatcgc aatctttttt ctgcgcacct cacggagttc ggcgtcggat
    12781 attttggcaa tatccacacc atcaatcagc acttgcccgc gggtgggttc aatcaggcga
    12841 ttgagaaggc gtaccattgt ggatttaccc gagccggata atcccatgat gacaaatatc
    12901 tcgccttctt caatggccag actggcgtct tttacgccaa gcgatagccc agttttttcc
    12961 agaatttgtt cttttgaaag tccttgttcg atatatttga acgctcgctg tggatgctcg
    13021 ccaaatatt
//
LOCUS       DAZFQD010000146        12932 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.146, whole genome shotgun sequence.
ACCESSION   DAZFQD010000146 DAZFQD010000000
VERSION     DAZFQD010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12932
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.146"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            264..764
                     /gene="tssB"
                     /locus_tag="ACHKTW_004239"
     CDS             264..764
                     /gene="tssB"
                     /locus_tag="ACHKTW_004239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HFO6465157.1"
                     /translation="MSKKFEGSVAPRERINISYVPKTDGQTAEVELPLNMLVVGDTGN
                     TQETSPLDERQAVSVNKHNFGAVMAEAAIGLNFTVPATLKGSTTDDELNVALNIKSLD
                     DFSPDSVARQVPEVNKLLELREALTALKGPMGNLPAFRTQLQALLENEESREQLLKEI
                     GLVSNK"
     gene            799..1023
                     /locus_tag="ACHKTW_004240"
     CDS             799..1023
                     /locus_tag="ACHKTW_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6465158.1"
                     /translation="MTAHKNISGTYHLSVADILQVVYQVCFSPSVEINQDGVAALITT
                     LDRRISDLLDEIIHFCEFQQSASHWQRVLH"
     gene            1074..2549
                     /gene="tssC"
                     /locus_tag="ACHKTW_004241"
     CDS             1074..2549
                     /gene="tssC"
                     /locus_tag="ACHKTW_004241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001501045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HFO6465159.1"
                     /translation="MSLQEEELVSSHAGQPEQASSLLDQIMAQTRIQPGSEGYDVARQ
                     GVTAFIASILQSTASAEPVNKLAVDSMIADIDERISRQMDVIIHAPAFQQVESFWRSL
                     KTMVDRVDFRENIKVNVLHVTKQELLEDFEFAPEIIQSGFYKHVYSSGFGQFGGEPIA
                     AVLGAYEFKNTAPDMKLLQYVSAVGAMAHAPFLSSVSPEFMGLNSWTELPNIKDLYAI
                     FEGPAYTKWRALRDSEDSRYLGLTAPRFLLRQPYSPTDNPVKNFNYYEDVSQNHEDYL
                     WGNTAWMLACNIADSFAKYRWCPNIIGPQSGGAVKDLPVHLFETMGQIQAKIPTEVLV
                     TDRREFELAEEGFITLTMRKDSDNAAFFSANSVQKPKHFPGKDAETNYKLGTQLPYLF
                     IINRLAHYIKVLQREQLGSWKERSDLERELNTWIRQYVADQENPPADVRSRKPLRAAK
                     VEVMDVEGEPGWYQVALSVRPHFKFMGANFELSLVGRLDRE"
     gene            2556..2969
                     /gene="tssE"
                     /locus_tag="ACHKTW_004242"
     CDS             2556..2969
                     /gene="tssE"
                     /locus_tag="ACHKTW_004242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HFO6465160.1"
                     /translation="MIPDRERTTGSLFERMEASSARNRQGGSIHSLRQSIRQNLRNIL
                     NTRSGSCRGAPELGIDEPEGAENFRESMSRAIEQCIERYEPRISHAEVQAVVSSASSP
                     LDMTFHITAWVTFNETHEVLEFDMAPNGSQHYRVD"
     gene            2973..4823
                     /gene="tssF"
                     /locus_tag="ACHKTW_004243"
     CDS             2973..4823
                     /gene="tssF"
                     /locus_tag="ACHKTW_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HFO6465161.1"
                     /translation="MEFEERYFREELDYLRQLSKLLATEKPHLARFLAEKDADPDIER
                     LLEGVAFLTGNLRQKIEDEFPELTHGLIKMLWPNYLRPVPAMTLIEYTPDMDKSSVPV
                     LIPRNEQFTTNAGEIRVDEVLPSDAKKEEPPPCTFTLCRDIWLLPVRLEQIENRSTTR
                     NGVINITFSVAPGTDFRTLDLNKLRFWLGNDNNYTRDQLYLWFCEYLQGADLTVGEQH
                     IRLPEFMLKAVGFEPQDAMLPWPKNVHSGYRILQEYFCYPDAFLFFDLCGCPALPDGL
                     QAEFFTLQLRFSRPLPVDIRLRRDSLRLYCAPAINLFIHHAEAITLDNRRADYPLVPS
                     RHYPQHYDVFSVNSVVSQVQDMFRKKDLGRPVSTQAARQWPAFESFSHQMEYSRKREV
                     VYWHHRTKTSLFHRGFDHTLAFIHADGSYPSDESLLSNEVVSVSLTCTNRELPSQIRS
                     GDITGTTGKNAAVASFRNITRPTQPLWPVIDGSLHWSLLSAMNLNYLSLLDTDALKQV
                     IANFDRHAIHHPQTARLSQQKLDAIERLETRPVDRLFTGIPVRGLASTLYLHPEPFVC
                     EGEMYLLGTVLSHFLSLYASVNSFHMLTVVNTESQETWKWTERIGQHPLI"
     gene            4787..5869
                     /gene="tssG"
                     /locus_tag="ACHKTW_004244"
     CDS             4787..5869
                     /gene="tssG"
                     /locus_tag="ACHKTW_004244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HFO6465162.1"
                     /translation="MDGKNRAASSYLSPGNPPADKEQNDPLAQVFHNACSYNFFAMAE
                     LLHRLAKGEKGTPELSLRDDPAQETLRFSADASLAFPCSDISALKRDTSGAFRMTTTF
                     MGLQGSQSPLPGYYLDHLAWKAVHEQSPVGDFLDMFSHRLTQFVWHIWRKYRYHISFR
                     NGGVDAFSQRMYSLVGLGHRQLRDKLAINHSKMLAYSGILANPGRSPEIICGLVSHCF
                     DLSEVTLQNWQRRKVDIEPDQQNSLGSYSLKNGEKLAGRSVLGNFVLGTRVPDLSGKF
                     QLSITSLTRKQFLSFLPSGENFLPLTMFVSFILRDQLAWDLHLGLAPEQVGAMRLGDN
                     KSALLGWTSFLGTPEERPSVTIRVRS"
     gene            5894..7174
                     /gene="tagH"
                     /locus_tag="ACHKTW_004245"
     CDS             5894..7174
                     /gene="tagH"
                     /locus_tag="ACHKTW_004245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated FHA domain
                     protein TagH"
                     /protein_id="HFO6465163.1"
                     /translation="MPEEKLQTLSLQVINGSELESGRAARCLFTQQGNVGHGPERHWS
                     VQDRQQSIPAQAFTVILHDGTFCLRPQTAQLWLNQAKVTATSDLIQLRQGDEIQIGRL
                     MVRVHLNRGDIPHYDEEMATPETIVTNRDMLTDTLLSTEGTPHYPGMTHRHQLADTVV
                     NGFSADPLQALQSESLITTGDPLSGIAAVRPSAPLSDPASNGGINTPFMDLPPIYASP
                     GDRNDDVSAAEMAQRHLAVTPLLRGLGGSLTMSNSDDADDFLEEAGRTLQAAIKGLLD
                     LQQQRNSLSDKHLRPLEDNPLRLNMDYATALDVMFAEGKSPVHLAAPAAVSESLRNVR
                     HHEEANRAAIVESLRVLLDAFSPQNLLRRFVQYRRSHELRQPLDDAGAWQMYSHYYEE
                     LASDRQQGFEMLFNEVYAQVYDRVLREKQREPEA"
     gene            7171..7695
                     /gene="tssJ"
                     /locus_tag="ACHKTW_004246"
     CDS             7171..7695
                     /gene="tssJ"
                     /locus_tag="ACHKTW_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HFO6465164.1"
                     /translation="MNRKAFLACVLMCVILTGCETAKKISEVIKNPDIQVGSLKEQPS
                     EITVTLLTEPDTNTNAEGESAAVDVQLVYLTDDSKLLAADYDQIASTPLPDVLGKNYI
                     DHQDFNLLPDTIKTLPPVKLDEKTQFIGVVAYFSDDQATEWKQIETVEGTGHHYRLLV
                     HVRQSSIEMKKEDE"
     gene            7698..9029
                     /gene="tssK"
                     /locus_tag="ACHKTW_004247"
     CDS             7698..9029
                     /gene="tssK"
                     /locus_tag="ACHKTW_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HFO6465165.1"
                     /translation="MATTRNKVMWQEGMLMRPHHFQQQQRYNDYLDNQRFRAMNDLSW
                     GFTELTLNNELLAQGKIMIDSASGTLPDGTVFSIPDQDALPDPLHPQHFPDERSRNIY
                     LALPVASDVRNEISDGRRIGRYRLNYADVRDLHSEEGDTRTLTLGQLTPRIMSGAEDM
                     SAYITLPLCRISDRHADGSLTLDDDFIPSCQNIQVSKKLRVYLKEVQGAIGGRASDLA
                     NRIGSPAQSGIADVAEFMMLQLLNRNQTRFTHHARRSQLHPEDFYLDLAGLLGELMTF
                     TEPSRLPCPLDVYDHHDLTKTFKTLLPEVKRALHTVLSPRAVNLPLHLRDGIWQADIH
                     DTELLQSATFVLAVAANMPVDQIQRQFIQQSKISSPEKIRNMVSVQIPGIPLRALMVA
                     PRQLPYHSGFSYFELDKSGQAWTEMAAAGAVALHVSGSFPDLNMQLWAIRG"
     gene            9034..9795
                     /gene="icmH"
                     /locus_tag="ACHKTW_004248"
     CDS             9034..9795
                     /gene="icmH"
                     /locus_tag="ACHKTW_004248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmH/DotU"
                     /protein_id="HFO6465166.1"
                     /translation="MDEGSLSLPPFTGYDEKSQRNYHLALRGNSLNPMIDAATPLLGM
                     VMRLSTMNSQTMPEHLFAQVVTDVQAVEQLLQEQGYEPGVIISFRYILCTFIDEAALG
                     NGWSNKNEWIKQSLLVHFHNEAWGGEKVFILLERLIREPKRYQDLLEFLWICFSLGFR
                     GRYKVAAQDQGEFEQIYRRLYHVLHKLRGDAPFPLLHQDKKTQGGRYQLISRLTVKHI
                     FCGGVVVLALFYLFYLLRLDSQTQDILHQLNKLLR"
     gene            9804..12569
                     /gene="tssH"
                     /locus_tag="ACHKTW_004249"
                     /gene_synonym="clpV"
     CDS             9804..12569
                     /gene="tssH"
                     /locus_tag="ACHKTW_004249"
                     /gene_synonym="clpV"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
                     /protein_id="HFO6465167.1"
                     /translation="MIQIDLPTLVKRLNLFSRQALEMAASECMSQQAAEITVSHVLIQ
                     MLAMPRSDLRVITRQGDIGMEELRQALTVENYTTARSADSYPAFSPMLVEWLKEGWLL
                     ASAEMQHSELRGGVLLLALLHSPLRYIPPAAARLLTGINRDRLQQDFVQWTQESAESV
                     VPDADGKGAGTMTDASDTLLARYAKNMTADARNGRLDPVLCRDHEIDLMIDILCRRRK
                     NNPVVVGEAGVGKSALIEGLALRIVAGQVPDKLKNTDIMTLDLGALQAGASVKGEFEK
                     RFKGLMAEVISSPVPVILFIDEAHTLIGAGNQQGGLDISNLLKPALARGELKTIAATT
                     WSEYKKYFEKDAALSRRFQLVKVSEPNAAEATIILRGLSAVYEQSHGVLIDDDALQAA
                     ATLSERYLSGRQLPDKAIDVLDTACARVAINLSSPPKQISALTTLSHQQEAEIRQLER
                     ELRIGLRTDTSRMTEVLVQYDETLTALDELEAAWHQQQTLVREIIALRQQLLGVAEDD
                     AAPLPDADTVEDTQPESESEQDNTGAVPADEADREQPEETAETVSPVQRLAHLTAELD
                     ALHNDRLLVSPHVDKKQIAAVIAEWTGVPLNRLSQNEMSVITDLPKWLGDTIKGQDLA
                     IASLHKHLLTARADLRRPGRPLGAFLLAGPSGVGKTETVLQLAELLYGGRQYLTTINM
                     SEFQEKHTVSRLIGSPPGYVGYGEGGVLTEAIRQKPYSVVLLDEVEKAHPDVLNLFYQ
                     AFDKGEMADGEGRLIDCKNIVFFLTSNLGYQVIVEHADDPETMQEVLYPVLADFFKPA
                     LLARMEVVPYLPLSKETLATIIAGKLARLDNVLRSRFGAEVIIEPEVTDEIMSRVTRA
                     ENGARMLESVIDGNMLPPLSLLLLQKMAANTAVARIRLSAVDGAFTADVEDAQNDESV
                     TKDETVL"
     gene            12566..>12932
                     /locus_tag="ACHKTW_004250"
     CDS             12566..>12932
                     /locus_tag="ACHKTW_004250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     TagO"
                     /protein_id="HFO6465168.1"
                     /translation="MSRLRVFRQTGWFIAGLMTGLPATAAPAEASSMAGVAVAVATTT
                     PPDATATLQAMQSCRRESAALERLDCYDRLLAPLSPSGFDGALVKAGFVGEAWTRATE
                     QEKHRQGNTTELLVTQVPGE"
BASE COUNT         3026 a         3549 c         3547 g         2810 t
ORIGIN      
        1 aaaaatccat atttaaatta ttgccataat acacctgacg cgaattgcac aggttcacac
       61 aatctattta acgcttgcag aatacccctc tttacgatcg ataaaaagtc cttttcttat
      121 ttattttgaa gcgtaaaatg cggggtgatg aaggagtggt gattttattt tcaccgccat
      181 tagggcggca ttgccgatat ttttttgcgg tacaaatatg ccgtgttcat ggcctgtacg
      241 agataacatg acggatcact tttatgagca aaaaatttga aggcagcgtc gcgcctcgcg
      301 agcgtattaa tatatcgtat gtcccaaaaa cagatggcca gacggctgaa gtggaacttc
      361 cgcttaacat gctcgttgtc ggcgataccg gaaatacgca ggaaacatcc ccactggatg
      421 aacgtcaggc agtttccgtt aacaagcaca atttcggtgc agtgatggct gaagcagcca
      481 ttggcctcaa ttttactgtc cccgccacgc tgaagggaag cacaacagac gatgaactga
      541 atgtggcgct gaatatcaaa tcactggatg atttttcacc agatagcgtg gcccggcagg
      601 ttccggaagt gaataaattg ctggaactgc gtgaagcact gactgcactg aaggggccga
      661 tgggtaacct tccggcattc cgtacccagc tccaggcatt gctggaaaat gaagagtccc
      721 gcgagcagtt gctcaaagaa atcggtctgg tgagcaataa ataaccacca ctatttcatt
      781 aaacagggtt atccaggcat gacggcacac aaaaatatct ccgggacata ccacctctct
      841 gttgcagaca ttctgcaggt ggtttaccag gtatgtttca gcccgtccgt tgaaatcaac
      901 caggatggtg tggctgcgct gattacaaca ctggatcgcc gtatcagtga tctattggat
      961 gagattatcc atttttgtga gtttcagcag agcgcgtcgc actggcagcg cgtacttcac
     1021 tgacggcgaa aagccggttt cagaaacaca gctaaaacag aaggattttc agtatgtctt
     1081 tacaggaaga ggaactcgta tccagtcatg ccgggcagcc tgagcaggca tcgtcgctgc
     1141 ttgatcaaat catggcacag acccgtattc agcccggttc ggaaggttat gacgttgccc
     1201 gccagggtgt gacagccttt attgccagca tcctgcaatc cactgcatct gccgaaccgg
     1261 tcaataaact ggccgtggac agcatgattg cagatattga tgaacgcatc agccgccaga
     1321 tggatgtcat catccatgcg cctgcctttc agcaggtcga gtcgttctgg cgctcactga
     1381 aaaccatggt ggatcgcgtt gatttccgtg aaaacatcaa ggtcaacgtg cttcatgtca
     1441 ccaagcagga acttctggaa gactttgagt ttgcgccaga aattattcag tccggatttt
     1501 ataagcacgt ttattcgtcc ggtttcggac agtttggcgg tgaacccatc gctgccgtgc
     1561 tgggcgctta tgagttcaaa aataccgcgc ctgacatgaa actgctgcaa tacgtcagtg
     1621 ccgtgggcgc aatggcgcac gcaccgttcc tgtcctccgt ttccccggag tttatggggc
     1681 tgaactcgtg gaccgaactg cctaatatca aagatcttta tgccatcttt gaaggtccgg
     1741 cctacaccaa atggcgcgcc ctgcgtgact cggaagattc gcgctattta gggcttacgg
     1801 caccccgctt cctgcttcgc cagccctatt caccaacaga taaccctgtt aagaacttta
     1861 actattacga agatgtcagc cagaaccacg aagattatct gtggggtaat acggcctgga
     1921 tgctggcatg caatatcgca gacagttttg ccaaataccg ctggtgtcca aacattatcg
     1981 gtccgcaaag cggcggcgca gtgaaagatc tgccggtgca tctgttcgaa acgatggggc
     2041 agattcaggc caaaatccca accgaggtgc tggtcaccga tcgccgcgaa tttgaactgg
     2101 ctgaagaggg ttttatcacc cttaccatgc gtaaagactc tgataatgca gcctttttct
     2161 ctgcaaactc ggtacaaaaa ccgaagcact ttccgggaaa agatgcagaa accaactata
     2221 agctgggtac gcagcttcct tatctcttca tcatcaatcg gttagcgcac tacatcaagg
     2281 tgttgcagcg tgaacaactg gggtcatgga aagaaaggag cgacttagaa agagagctta
     2341 atacctggat ccgtcagtat gttgccgacc aggaaaaccc gcctgcagac gtgcgcagcc
     2401 gcaaacctct gcgcgctgcc aaagttgagg ttatggatgt agaaggcgaa cccggctggt
     2461 accaggttgc gctaagcgtg aggcctcatt ttaaatttat gggggcaaat tttgagcttt
     2521 ccctggttgg ccggttagac agggagtaac acaccatgat accggatcgg gaaagaacca
     2581 caggttctct ttttgagcgt atggaagcct cttcggccag aaaccggcag ggaggcagta
     2641 ttcattcgct caggcaatcc attcgccaga acctacgaaa cattcttaat acccggtccg
     2701 gtagttgccg gggtgcgcct gagctgggca ttgatgaacc ggaaggggct gaaaacttcc
     2761 gcgaatccat gagtcgggcc atcgagcaat gtatagaacg ctatgaaccc cggatctcac
     2821 acgctgaagt gcaggcagtg gtttcatccg cctccagccc gctggatatg acgtttcata
     2881 ttacagcctg ggtgacattt aacgaaacgc atgaggtgct tgaatttgat atggcaccga
     2941 atggcagcca gcattaccgg gtggactgac aaatggaatt tgaagaacgc tatttccggg
     3001 aagaactcga ttacctgcgc cagcttagca agctgctggc aacggaaaaa ccccatctgg
     3061 cccgcttcct ggccgaaaaa gatgcggatc cggatattga acgcctgctg gaaggggtgg
     3121 cttttcttac cggcaatctc cgccagaaaa ttgaggatga attcccagaa ctgacgcacg
     3181 ggcttattaa gatgctatgg cctaattacc tgcgtccggt tccggcaatg acccttattg
     3241 aatatacgcc ggatatggat aagtcttctg taccggtgtt aatcccccgt aatgagcagt
     3301 ttacaaccaa cgccggggaa atcagagttg atgaagtgct gccctctgat gctaaaaagg
     3361 aggagccgcc tccctgtacc ttcactctct gccgggatat ctggctgctg cccgttcgcc
     3421 tggagcagat tgaaaaccgc agtacgaccc gtaatggtgt tatcaacatc accttttcgg
     3481 tcgcaccggg aacggacttc cgcacgctgg atctgaacaa acttcgcttc tggctcggca
     3541 atgacaacaa ctatacccgt gaccagcttt atttatggtt ctgcgaatac ttgcagggtg
     3601 ccgacctgac tgtgggtgaa cagcatattc gcctgcctga gtttatgcta aaagctgtcg
     3661 gttttgagcc gcaggatgcc atgctgccct ggccgaaaaa cgtccacagc ggctaccgga
     3721 tccttcagga gtatttctgt taccccgatg cgtttctctt ttttgatctt tgtggttgtc
     3781 cggctttgcc tgacggattg caggcggaat tctttaccct gcaactgcgt ttttcgcgcc
     3841 ctttgcccgt ggacatccgg ctgcgccgcg attccctgcg cctgtattgc gcacctgcca
     3901 ttaatttatt tatccaccat gcagaagcca tcacgctgga caaccggcgg gcagactatc
     3961 cgctggttcc cagccgccat tacccacaac attacgatgt attttccgtt aacagtgtgg
     4021 tgagccaggt ccaggatatg ttcaggaaaa aagatctggg gcgtcctgtt tcgacgcagg
     4081 ccgcgcgcca gtggccagcc tttgaaagtt tcagccatca gatggaatac agccggaagc
     4141 gggaagtggt gtactggcat caccggacca aaacatccct gttccatcgc ggctttgatc
     4201 atacccttgc ctttatacat gctgatggca gttatccgtc agacgaatct ctgctcagta
     4261 atgaagtggt ttcggtatcg ctgacctgta ccaaccgtga gcttccgtca caaattcgtt
     4321 ccggcgatat caccggcaca accggtaaaa atgcggctgt tgcttcattt cgcaacatta
     4381 cccgcccgac gcaaccactc tggccggtca ttgatggcag cctgcactgg tccctactct
     4441 ccgccatgaa cctgaattat ctgtcattac tggatacgga cgcgctgaag caggtcatcg
     4501 ccaactttga tcgccacgca atccatcatc cgcagacggc acggctgtca caacaaaagc
     4561 tggatgccat tgagcgtctg gagacccgcc ccgttgatcg cctgtttacg ggtattcccg
     4621 tccggggact ggcctccacg ctgtatctgc acccggagcc gtttgtctgt gaaggggaaa
     4681 tgtatctgct cggtacggtg ctttcgcatt ttctgtcgct gtacgccagc gttaactcat
     4741 tccacatgct gaccgttgtg aacacagaaa gccaggagac atggaaatgg acggaaagaa
     4801 tcgggcagca tcctcttatc tgagtccggg aaatcccccg gcagataaag agcagaatga
     4861 tccgctggca caggttttcc ataatgcctg ctcttacaat ttttttgcga tggcggagct
     4921 gttgcaccgc ctggcaaagg gtgaaaaggg gacgccagaa ttatccctgc gtgacgatcc
     4981 ggcacaggaa accctgcgtt tcagcgccga tgccagcctt gccttcccgt gcagtgatat
     5041 cagtgcactg aaacgggata cgtcaggggc attcaggatg accaccacct ttatggggtt
     5101 acaggggagc cagtcgcctc tccccggtta ttacctcgat cacctggcct ggaaagcggt
     5161 acatgaacaa agtccggtag gtgattttct ggatatgttc agccaccgcc tgacccagtt
     5221 tgtctggcat atctggcgta agtaccgcta tcacatcagt tttcgtaacg gcggtgtgga
     5281 tgcgttctcg cagcgcatgt actcccttgt cgggctgggc caccgccagc ttcgcgataa
     5341 gctggcgatt aatcacagca aaatgctggc gtattccggg atcctggcaa atccgggacg
     5401 atccccggaa ataatctgtg gcctggtgtc ccactgcttt gatttgtcag aagtgacgct
     5461 gcaaaactgg caacgtcgca aggtggatat tgaaccggat cagcaaaata gtctggggag
     5521 ctattctctt aaaaatggcg aaaaactggc gggaagatcg gtactgggca actttgttct
     5581 gggtacacga gtgcccgatc tcagcggaaa attccagctc agtattacca gcctgacccg
     5641 aaaacagttc ctctcttttc tgccgtccgg cgaaaacttc ctgccactga cgatgttcgt
     5701 gtccttcatc ctgcgcgatc agcttgcctg ggacttacat ctggggctgg cgccggagca
     5761 ggtgggcgca atgcgcctgg gcgataacaa aagtgcgctg ctgggctgga ccagcttcct
     5821 cggcacaccg gaagaacgac cctcagtcac tatcagggta cggtcgtaaa ttatgataat
     5881 cacggagcag aatatgccgg aagagaaact acagacgctt tcattgcagg tcattaacgg
     5941 cagtgagctg gaaagcggac gggcggcgcg ctgtctgttc acacagcagg gaaatgtcgg
     6001 ccatggcccc gaacgccact ggtcggtaca ggatcgtcag cagagcattc cggcccaggc
     6061 ttttaccgtt atcctgcacg atggcacatt ttgtctgcgc ccgcagacgg cacaactgtg
     6121 gctgaatcag gcaaaagtca cagcaacatc agacctaata cagttgcgcc agggcgatga
     6181 gatccagatc ggacggctga tggtgagggt tcatctgaac cggggagata ttccccatta
     6241 cgatgaggaa atggccactc ccgaaaccat cgttaccaat cgcgatatgc tcacggatac
     6301 cctgctatca acggagggta cgccacacta tccgggaatg actcaccggc accaacttgc
     6361 agacaccgtg gtaaacggtt tttctgccga tccactccag gcacttcagt ccgaaagcct
     6421 gattaccacg ggcgatccgc tttcaggcat tgcggctgtc cggccatcgg caccgctgtc
     6481 cgatccggca agtaatgggg ggatcaatac tccgtttatg gatctgccgc ccatttatgc
     6541 cagccccggc gatcgtaatg acgacgtctc tgcggcagaa atggcgcaac gccaccttgc
     6601 ggtcaccccc ttactgcgcg gtcttggcgg ctcgcttacc atgagcaatt ccgacgatgc
     6661 ggatgatttt ctggaggagg ccggacgaac gttacaggcc gcaataaaag gtctgctcga
     6721 tttgcagcag cagcgtaaca gcctctcaga caaacatttg cgcccgctgg aagataaccc
     6781 gctgcgcctg aacatggatt acgccaccgc gctcgacgta atgtttgccg aaggtaaaag
     6841 cccggtacat ttggcggctc ccgccgccgt cagtgaaagc ctgcgcaatg tccgccacca
     6901 cgaagaagct aaccgggcag cgattgtgga gtcgcttcgt gtcctgctgg atgctttctc
     6961 accacaaaat ctgctgcgcc gctttgtgca gtaccgccgc agccatgaac tgcgccagcc
     7021 gctggatgat gccggagcat ggcaaatgta cagccattat tacgaagaac tggcctccga
     7081 tcgccagcag gggtttgaga tgctgtttaa cgaggtctac gcccaggtct atgaccgggt
     7141 gcttcgtgaa aaacagcggg agccggaagc atgaacagga aggcgtttct ggcctgcgtg
     7201 ctgatgtgcg tcatattaac cggctgcgaa acggcgaaaa aaatcagtga ggtgatcaaa
     7261 aacccggata tccaggtcgg gagcctgaag gagcaacctt cggaaataac cgtcaccctg
     7321 ctgaccgaac cggacaccaa taccaatgcg gaaggtgaat cagccgccgt ggatgtgcaa
     7381 ctggtgtacc tgactgacga ctcaaaattg ctggcagccg actatgacca gatagccagt
     7441 acgccgctac cggatgtgct gggtaaaaac tatatcgacc accaggattt caacctgttg
     7501 ccggacacca tcaaaaccct gccgccggta aagctggatg agaaaacgca gtttattggt
     7561 gtcgtggcct atttctccga tgaccaggcc actgaatgga aacaaatcga gacggtggaa
     7621 ggaaccggcc accattaccg cctgctggtg catgtacgtc aaagcagcat cgaaatgaaa
     7681 aaagaggatg aataattatg gcaaccaccc gcaacaaggt gatgtggcag gaagggatgc
     7741 tgatgcgtcc acaccatttc cagcagcagc agcgttacaa cgactacctg gataaccagc
     7801 gtttccgggc catgaatgat ttatcctggg gatttaccga actcactctc aacaatgaac
     7861 tgctggcaca gggtaagatc atgattgaca gcgcgtcagg cacactgccc gacggcaccg
     7921 tcttttctat ccccgaccag gacgcactgc ccgatccgct gcacccgcaa cattttccgg
     7981 acgagagaag ccgcaatatc tacctcgctc tgccggtcgc cagtgatgtg agaaatgaaa
     8041 tcagcgacgg gcggcgaatc gggcgttacc ggctgaatta tgccgatgtc cgggatttgc
     8101 attcagaaga aggtgacacg cgaacactga cgctgggaca actgacgccg cgcattatga
     8161 gcggtgcaga agatatgagc gcctatatta cgctcccgct ttgccgtatc agcgatcgcc
     8221 atgccgacgg ctccctgacg ctggatgacg attttatccc ctcctgccag aatattcagg
     8281 tcagtaagaa actgcgtgtt tatctcaaag aggtacaggg ggccattggc ggacgggcaa
     8341 gcgatctggc aaaccgcatt ggctctccgg cgcagagcgg catcgcggat gtggcggaat
     8401 ttatgatgtt gcagttactt aaccgtaacc agacccggtt tacccatcac gcccgtcgat
     8461 cccagctcca cccggaagat ttctaccttg atcttgccgg gttgctgggt gaactgatga
     8521 cctttaccga gccgtcgcgc ctgccctgcc cgcttgatgt gtatgatcat catgacctga
     8581 ccaaaacatt taaaacgctg ttaccggaag tcaaacgggc gctgcatacc gtactgtcgc
     8641 caagagccgt caatctgccg ctgcatctgc gtgacggtat ctggcaggcc gatatccatg
     8701 acacagaact gctgcaatct gccacctttg tgctggccgt ggcggcaaac atgccggtcg
     8761 atcagatcca gcgccagttt atccagcagt cgaaaatttc ctcgccggaa aaaatccgca
     8821 atatggtcag tgtgcagatc cccggcattc cattgcgcgc actgatggtg gctccccgcc
     8881 agcttcctta ccattccggg ttcagctatt tcgaactcga caagagcgga caggcctgga
     8941 cagaaatggc tgccgccggg gccgttgcac tgcatgtatc cggcagtttc ccggatctga
     9001 acatgcaact gtgggcgata agagggtaag acgatggatg aggggtcact gtctctgcca
     9061 ccatttaccg gatacgacga aaaaagccag cgcaactacc atctcgcgct tcgtggcaac
     9121 agcctgaatc cgatgatcga cgcggccacg ccgctactcg gtatggtaat gcgcctttca
     9181 accatgaaca gccagacgat gccggagcat ctttttgctc aggtggtgac ggatgttcag
     9241 gccgtggagc aactgcttca ggagcagggc tacgaaccgg gcgtgattat ctcgtttcgc
     9301 tacatcctct gcacgtttat tgatgaagcg gcgctcggta acggctggtc aaacaagaac
     9361 gagtggatca agcagtcatt gctggtccat ttccacaacg aagcctgggg tggagagaaa
     9421 gttttcatcc tgctggagcg cctgatccgt gaacccaaac gttaccagga tctgctggaa
     9481 tttctctgga tctgtttttc tctgggattt cgcgggcgct acaaagtggc agcacaggat
     9541 cagggggaat ttgagcagat ctaccgccgt ctgtaccacg ttctgcataa actgcgcggc
     9601 gatgcccctt tcccgctgtt acaccaggac aaaaaaaccc agggcgggcg ttatcagctc
     9661 atcagtcgcc tgactgttaa acatattttt tgtggtggcg ttgtcgtgct ggcgttgttt
     9721 tacctgttct atctgttgcg gctggacagc cagacgcagg acatcctgca ccagctaaac
     9781 aaattacttc gataaggacg tttatgatcc agattgatct tcccacgctg gtaaaacggc
     9841 tgaacctgtt ctcccgccag gcgctggaga tggccgcctc tgaatgtatg agtcagcagg
     9901 cagcggaaat taccgtcagc catgtgctca ttcagatgct cgccatgcca cgcagtgacc
     9961 tgcgggttat tacccgccag ggcgatattg gcatggaaga gttgcgccag gcgctgacgg
    10021 tagagaacta cacaaccgcc cgttctgcgg acagctaccc ggcgttttcc ccgatgctgg
    10081 ttgagtggct gaaagagggc tggctgctgg cgtcggctga gatgcagcac agcgaactgc
    10141 gcggcggcgt gttgctgctg gccctgctgc attcgccgct gcgttatata ccgcctgctg
    10201 ccgcccggct gttgaccggc attaaccgtg accgtctgca acaggacttt gtgcagtgga
    10261 cacaggagtc ggcggaatca gtcgtgccgg atgcagacgg taaaggcgca ggcacaatga
    10321 cggacgcctc tgacaccctg cttgcccgct atgccaaaaa catgaccgca gacgcccgta
    10381 acggcaggct tgacccggta ctgtgccgcg accacgaaat cgacctgatg atcgacattc
    10441 tctgccgccg ccgtaaaaac aacccggtgg tggtgggcga agcgggcgtg ggcaaaagcg
    10501 cactgattga agggctggcg ctgcgcatcg tggcaggcca ggtgccggac aagctgaaaa
    10561 acaccgatat catgaccctt gacctgggcg cattgcaggc cggggcgtcg gtgaagggtg
    10621 aattcgaaaa acgtttcaaa gggctgatgg cggaggtcat ttcctccccg gtgccggtca
    10681 ttctgtttat cgacgaagca cataccctga ttggcgcggg caaccagcag ggcgggctgg
    10741 atatctccaa cctgctcaaa ccggcgctgg cgcgcggcga gctgaaaacc atcgccgcca
    10801 ccacctggag cgagtacaaa aaatacttcg aaaaagatgc cgccctgtcg cgccgcttcc
    10861 agttggtgaa ggtcagcgaa cccaacgctg ccgaagccac cattattctg cgcggtctgt
    10921 cggcggtcta tgaacagtct cacggcgtgc tgattgatga tgacgccttg caggccgctg
    10981 cgacattaag cgagcgttat ctctccgggc gtcagttacc ggacaaagcg attgatgtgc
    11041 tggataccgc ctgcgcccgt gtggccatca acctgtcgtc gccgccgaag caaatctcgg
    11101 cgctgaccac tctgagccac cagcaggagg cggaaattcg ccagcttgag cgcgagcttc
    11161 gcatcggact gcgtaccgac acatcacgga tgaccgaggt gctggtgcag tatgatgaaa
    11221 cgctgacggc gctggatgaa ctggaagcgg cctggcacca gcagcagacg ctggtccggg
    11281 agattattgc gctgcgccag cagttactgg gcgtggcaga ggatgatgcg gcgccgttgc
    11341 cggacgcaga taccgtggag gatacgcagc cagagtcaga gtcagaacag gataataccg
    11401 gtgccgtacc ggctgatgag gccgacagag aacagccgga agagaccgct gaaacagttt
    11461 ccccggtaca acgtctggca catctcactg ccgaactgga cgccctgcat aacgaccggt
    11521 tgctggtttc cccgcacgtc gataaaaaac agattgcggc ggtgattgcc gaatggaccg
    11581 gcgtgccgct taaccgcctg tcgcagaatg aaatgtcggt catcaccgac ctgccaaaat
    11641 ggctcggcga caccatcaaa ggccaggacc tggcgattgc cagcctgcac aaacacctgc
    11701 tgaccgcacg cgccgacctg cgccgtccgg gacgcccgct gggcgcgttc ctgctggctg
    11761 gccccagcgg cgtgggtaaa actgaaaccg tcctgcaact ggcagagctg ctctacggcg
    11821 gtcgccagta cctgaccacc atcaatatgt ccgagttcca ggagaaacat accgtctcgc
    11881 gactgattgg ttcgcctccg ggctacgttg gctacggtga aggcggcgta ctgaccgaag
    11941 cgattcgcca gaaaccctac tcggtagtac tgctcgatga agtggaaaaa gcgcacccgg
    12001 atgtgctcaa cctgttctac caggcgttcg ataagggcga aatggcagac ggtgaaggcc
    12061 gcctgattga ctgcaaaaat atcgtcttct tcctgacgtc caacctcggc taccaggtaa
    12121 tagtcgagca tgccgatgac ccggaaacca tgcaggaagt actgtatccg gtgctggccg
    12181 acttcttcaa acctgccctg ctggcgcgta tggaagtggt gccgtacctg ccgctgtcga
    12241 aagagacgct cgccaccatc attgccggga aactggcccg cctggataac gtgctgcgca
    12301 gccgctttgg cgcggaggtg attatagaac cggaagtgac ggacgaaatc atgagccgcg
    12361 tcacccgcgc ggaaaacggc gcgaggatgc tggaatcggt catcgacggc aatatgctgc
    12421 cgccgctctc cctgctgctg ttgcagaaaa tggcggcgaa tacggcggtt gcccggattc
    12481 ggttgtcggc agtggacggc gcatttacgg cagacgtgga agatgctcag aacgacgagt
    12541 ccgtcacaaa ggatgaaacg gttttatgag caggttgcgc gttttccggc agacaggatg
    12601 gtttatcgcg ggcctgatga cgggcttacc cgccactgcc gcgcccgcag aagcatcgtc
    12661 aatggccggt gtggcggtcg ccgtcgccac cacgaccccg ccggatgcga cagccacgtt
    12721 acaggccatg cagtcctgtc ggcgggagtc cgcggcgctg gagcggcttg actgctatga
    12781 ccgcctgctg gcaccgctgt ccccgtcagg gtttgacggg gcgctggtca aagccggttt
    12841 tgtgggcgaa gcctggaccc gcgccacgga acaggaaaaa caccgccagg gcaacaccac
    12901 agagctgctg gtgacgcagg tgccggggga gc
//
LOCUS       DAZFQD010000147        12876 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.147, whole genome shotgun sequence.
ACCESSION   DAZFQD010000147 DAZFQD010000000
VERSION     DAZFQD010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12876
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.147"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            229..810
                     /gene="mdaB"
                     /locus_tag="ACHKTW_004251"
     CDS             229..810
                     /gene="mdaB"
                     /locus_tag="ACHKTW_004251"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HFO6465169.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYDVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGGLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            841..1155
                     /locus_tag="ACHKTW_004252"
     CDS             841..1155
                     /locus_tag="ACHKTW_004252"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HFO6465170.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(1203..3095)
                     /gene="parE"
                     /locus_tag="ACHKTW_004253"
     CDS             complement(1203..3095)
                     /gene="parE"
                     /locus_tag="ACHKTW_004253"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFO6465171.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(3124..3705)
                     /gene="yqiA"
                     /locus_tag="ACHKTW_004254"
     CDS             complement(3124..3705)
                     /gene="yqiA"
                     /locus_tag="ACHKTW_004254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HFO6465172.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(3705..4532)
                     /gene="cpdA"
                     /locus_tag="ACHKTW_004255"
     CDS             complement(3705..4532)
                     /gene="cpdA"
                     /locus_tag="ACHKTW_004255"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004115 - 3',5'-cyclic-AMP
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HFO6465173.1"
                     /translation="MESLLTLPLAGEARVRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPYQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            complement(4557..4979)
                     /locus_tag="ACHKTW_004256"
     CDS             complement(4557..4979)
                     /locus_tag="ACHKTW_004256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HFO6465174.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(4980..5609)
                     /gene="nudF"
                     /locus_tag="ACHKTW_004257"
     CDS             complement(4980..5609)
                     /gene="nudF"
                     /locus_tag="ACHKTW_004257"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HFO6465175.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            5814..7295
                     /gene="tolC"
                     /locus_tag="ACHKTW_004258"
     CDS             5814..7295
                     /gene="tolC"
                     /locus_tag="ACHKTW_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HFO6465176.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLAREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            7443..8114
                     /locus_tag="ACHKTW_004259"
     CDS             7443..8114
                     /locus_tag="ACHKTW_004259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HFO6465177.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            8120..9280
                     /locus_tag="ACHKTW_004260"
     CDS             8120..9280
                     /locus_tag="ACHKTW_004260"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HFO6465178.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDDIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFIE"
     gene            complement(9318..10106)
                     /gene="ygiD"
                     /locus_tag="ACHKTW_004261"
     CDS             complement(9318..10106)
                     /gene="ygiD"
                     /locus_tag="ACHKTW_004261"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HFO6465179.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPMLAQHLVELLAPVPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWAMSFNEYVKANLTWQGAVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGAWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            10249..11022
                     /gene="zupT"
                     /locus_tag="ACHKTW_004262"
     CDS             10249..11022
                     /gene="zupT"
                     /locus_tag="ACHKTW_004262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HFO6465180.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQPLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(11080..11250)
                     /gene="yqiD"
                     /locus_tag="ACHKTW_004263"
     CDS             complement(11080..11250)
                     /gene="yqiD"
                     /locus_tag="ACHKTW_004263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqiD"
                     /protein_id="HFO6465181.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
     gene            complement(11511..12164)
                     /gene="ribB"
                     /locus_tag="ACHKTW_004264"
     CDS             complement(11511..12164)
                     /gene="ribB"
                     /locus_tag="ACHKTW_004264"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HFO6465182.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(12268..12416)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            12538..12828
                     /gene="ubiK"
                     /locus_tag="ACHKTW_004265"
     CDS             12538..12828
                     /gene="ubiK"
                     /locus_tag="ACHKTW_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HFO6465183.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLALLEQRMSELENRSTEIKKQPDPETLPPTL"
BASE COUNT         3255 a         3445 c         3152 g         3024 t
ORIGIN      
        1 tcccaaaaaa aaccgaacca aacgtgtaaa ttattccggt gcgagtaaga attatgagga
       61 atggctatca gtattgtcat tttcagaaaa tatttatcct gcatcggtga gtcagagcaa
      121 gatcagactt ttgctaaatt cgcaaaagac tttgcacatt ttgctaattt caccgtaccg
      181 ctctgtgacg tactatagtc ggcaaacgtc tcaccttgag gttaaaaaat gagcaacatc
      241 ctgattatca acggcgcgaa aaaattcgcc cactccaatg gtcaactgaa cgacaccctg
      301 accgaagtcg cggatggcac actgcgcgac ctcgggcatg atgtccgcat cgttcgcgcc
      361 gacagcgact acgatgtcaa agcggaagta caaaactttc tctgggctga tgtggtgatc
      421 tggcagatgc caggctggtg gatgggcgcg ccgtggacag tgaaaaaata tattgatgat
      481 gtattcaccg aaggtcatgg aacgctgtat gccagcgatg gccgtacccg caaagatccg
      541 tcaaaaaaat acggttccgg cggcctggta cagggcaaaa aatatatgct ttctctgacc
      601 tggaacgcac caatggaagc cttcaccgaa aaagatcaat tcttccacgg cgttggcgtc
      661 gacggtgtgt atctgccgtt ccataaagca aaccaattcc ttggcatgga accgctgccg
      721 acatttatcg ctaatgacgt gataaaaatg cctgatgttc cccgctatac tgaagaatat
      781 cgcaagcatc ttgtggaaat ttttggttaa ctagagctca ggctttagaa ggagttaacc
      841 atgcttaccg taatcgcaga aatccgtact cgtcctggtc aacatcaccg tcaggcggta
      901 ttggatcagt ttgctaaaat cgttccaacc gtactgaaag aagaaggttg ccacggctat
      961 gcgccaatgg tggattgcgc tgctggtgtg agtttccagt ctatggcacc ggattctatc
     1021 gtgatgattg agcagtggga aagcatcgcg catcttgaag cgcatctgca aaccccgcac
     1081 atgaaggcgt acagcgaagc cgtaaaaggc gacgtgctgg agatgaatat ccgcatcctg
     1141 caacctggga tttaaacctg ccttgtttgc ccggccatcc tgaccgggca atgttctttc
     1201 ctttaaacct caatctccgc catgtcgcct ttctcttgca accagttgcg gcgatcttcc
     1261 gagcgtttct tcgccagcag catatccatc atcgcgtcag tacgctgatc gtcttcatca
     1321 tcgatagtca actgcaccag acggcgagtg ttcggatcaa gcgtggtttc acgcaattgc
     1381 atcgggttca tttcccccag acctttaaaa cgctggacgt tcggcttgcc tttcttgcgt
     1441 tttaattgct caagtacgcc ctctttctct tcttccgtca gcgcgtaata aacctctttc
     1501 ccgagatcaa tacggtagag cggtggcagt gcgacgtaaa cgtgaccgtg tttcaccaac
     1561 gcgcggaagt gttttacgaa caaagcgcag agcagcgtgg caatgtgcag accatcagag
     1621 tccgcatccg cgaggataca gattttgcca taacgaagct ggctcagatc gtcgctgtca
     1681 ggatcgatac cgatcgctac cgaaatatca tgcacttcct gcgaggccag cacttcgtcg
     1741 gaagaaactt cccaggtgtt aaggatctta cctttcagtg gcatgatcgc ctgatattcg
     1801 cgatcgcgcg cctgcttggc agatccgcct gcggagtcac cttccacaag gaacagttcg
     1861 gtacggttaa gatcctgcgc ggtacagtca gccagtttgc caggcagtgc tgggccgctg
     1921 gtcagctttt tgcgcaccac ttttttagcc gcacgcatac ggcgctgggc gctggaaatc
     1981 gccatctccg ccagcagctc cgccgcctga acgttctggt tcagccacag gataaaggca
     2041 tctttcacca cgcctgaaac gaatgccgcg cactgacgcg aagagagacg ctctttggtc
     2101 tgcccggcaa actgcggatc ctgcattttt actgacagca cataggcgca gcgatcccag
     2161 atatcttccg ccgacaactt tacaccgcgc ggcagaatgt tgcggtattc acagaactca
     2221 cgcatcgcgt ccaacaggcc ctgacgcaga ccgttaacgt gcgtaccgcc ctgcatcgtt
     2281 gggataaggt tgacgtagct ttcggtcagc agttcaccgc cttccggcag ccacagtagc
     2341 gcccagtcca cagcttcagt atcaccagcg aaattaccga taaacggttt ttctggcagc
     2401 gtcggtaaac cgtttaccgc ttccgccagg taatcattca gaccgtcctg gtagcaccag
     2461 cgctgttcgg tgttgttgat ctcatcttta aaggtaatct caacgccagg gcacagtact
     2521 gctttggcct tcagcacatg cgtcaggcgt gaaacagaaa aacgcgggct gtcaaagaag
     2581 gtttcatccg gccagaagtg cacgctggta ccagtattgc gtttaccgca agtgccgaca
     2641 acctgtaaat cctgcacctt ttcgccattt tcaaaggcga tgttataaac ctgaccatcg
     2701 cggcgcacgt taacttctac gcgcttcgac agggcgttaa ccaccgaaat ccccacgcca
     2761 tgcaggccgc cagagaactg gtaatttttg ttagagaatt taccgcctgc atgcagacgg
     2821 caaagaatca gttcaaccgc cggtacaccc tcttccgggt gaatatccac cggcatcccg
     2881 cgcccatcgt caataacttc taacgactgg tcagcatgta agattacatc cacgcgtttt
     2941 gcgtgacccg ccagtgcttc atcgacactg ttatcaataa cttcttgccc caaatggtta
     3001 gggcgagtgg tatcggtata catccccgga cggcggcgaa ccggctcaag cccggtgagt
     3061 acctcaatgg catcagcgtt ataagtttgc gtcatggttt aagttagcaa ttcgagttga
     3121 tcgtcagaga tggtgcagac caagaaaatc gacgatcggg ttgaaataat cttcgaagcc
     3181 cgtgaatgcg tggttgccgc cttctatgac agtctggcgg caggaagcgt agtacgccac
     3241 cgcctggcgg taatccagca cttcatctcc cgtctgttgc agcagccaga tcaaatccgg
     3301 cgcttccagc gggtcaatct gcatgacttt gagatcgtaa atatggcgtg actctagcac
     3361 atattgctgc ccggtgtagg ggttctcgtt ctgaccgaga tagtccgtca gcagttcaaa
     3421 cgggcgcacc gccgggttta ccaccactgc gggcagcata aaacattgtg acaaccaggt
     3481 ggcgtaatat ccccccagtg acgaaccgac aatacccagc gaatcgccgc catgttccag
     3541 gacaatggat tccagcagct ctgccgcgtc ggaaggatac ggcggcaact gcggaatgat
     3601 catctcaacg tcagggtgat gttccgccag ccagtttttt aacaagctcg cttttgcaga
     3661 gcgcggcgag ctgttgaaac cgtgtaaata aagaagcgta gacatcagta gccttctgaa
     3721 gcggtatcag gttggaaacg tgtgtccgcc aggcgatgca cctcggtggt cagcgtgcca
     3781 tcagcatgta actcgagagt acgccagccg ggcgcgatag tatccagcgt aaagttggaa
     3841 cagtgcggct taaactgcac acaggtcgac ggcgttgcca gcaggcggcg accgttccag
     3901 tcgagatcca gctcctgatg aatatgaccg cacagcaagt atttgacgtg cggaaacttc
     3961 gccagcacgg tatccagttc gcccgcgtta cgcagactgt gttgatcgag ccaactacaa
     4021 cccgcaggta gcggatgatg atgcagcagc agcaacgtat ggcgttctgg cgcatcggcc
     4081 agtttacgtt ccagccactc aagctgaaac tcgctcagct caccgtgcgg cacgccaaac
     4141 acctggctat ccagcaacag gatttgccat tgctcaccaa taaacacgcg cttcgccggg
     4201 gagatacccg catcctgtaa cgcgctgtac atcgcgggct ggaaatcgtg gttgcccggc
     4261 agccagacgc agggcgcacg aaaacttgcg atgccttcag cgaaatgctg ataggccgca
     4321 gaggattgat cctgcgctaa atcacctgtc gcgacaatca ggtcgaattc gtgctgatat
     4381 ggacgaatcg cctccagcac cgcctggtag ctttcccagg tgtttacccc taacagggct
     4441 tcgtgctttt gtgcaaacag gtgagtatcg gtaatttgta aaatcctgac tctggcctca
     4501 ccagccagag gaagggttaa caggctttcc aaatggtgtc cttaggtttc acgacgctag
     4561 taaaccggaa tcgccatcgc tccatgtgct aaacagtatc gcaaccagtc cgctaaaaac
     4621 tggttaattt gatgcttttc gtcgcgttga tgcaattttt tattaggata atcataccgc
     4681 gctttgaagc gaaaaatctg ctggcttgaa cacacttcag ccaccatcgc gtcatgatac
     4741 agacgcaccg tcattgacgg aaggctccag taactgatcg cgggcgcagt ctgttctatt
     4801 gtcaccaggg tagtgtatcg ggtcgattcc acaatcgtca gccgatattg tgcgtttgcc
     4861 acctgatagc ttacagtttc gccgggtgcg tcattgcgcg gtaacaaacg gcgcaattgt
     4921 gaaaagttca tctcgcacag gcgcatcatt tcaggaaagt caggtgtata acgcttcatt
     4981 tatgcccact cattttttaa cgcttgatga tgcagctgca gccattgcaa agcgatgacc
     5041 gacgctgcgt tgtcgatttt cccctcttct acccactggt atgcctgttc ccggcttacc
     5101 acatgaacgc gaatatcttc gttttcatca gccagaccat gaataccgct tgcggtcgtg
     5161 gcgtccactt cgcccaccat aattgacgaa cgctcactgg tgccccccgg gcttgccagg
     5221 aaacttaaca ccggtttggt ccgtttgact atcagtcccg cctcttcaat cgcttcgcga
     5281 cgggcaacat cttccacact ttcaccctct tctatcatcc cggcaaccat ctccagtagc
     5341 cagggggttt cactggtgtc gtatgcggca atccgaatct gctcaatcag cacaacttca
     5401 tcacgcactg ggtcaaaggg tagcaagact gcggcgtgac cgcgctcaaa aatttccctc
     5461 cgtacctcat gactcatttg cccgttgaat aaacgatgac gaaatctata aagatctaat
     5521 gaaaaaaagc cgcgataaag tgtttctcgt gcaataattt ctacatcgtt tttgccaaat
     5581 gtaacgggca ggttgtctgg cttaagcatt gttaatgtcc tggcactaat agtgaattaa
     5641 atgtgaattt cagcgacgtt tgactgccgt ttgagcagtc atgtgttaaa ttgaggcaca
     5701 ttaacgccct atggcacgta acgccaacct tttgcggtag cggcttctgc tagaatccgc
     5761 aataatttta cagtttgatc gcgctaaata ctgcttcacc acaaggaatg caaatgaaga
     5821 aattgctccc cattcttatc ggcctgagcc tttctgggtt cagttcgttg agccaggccg
     5881 agaacctgat gcaagtttat cagcaagcac gccttagtaa cccggaattg cgtaagtctg
     5941 ccgccgatcg tgatgctgcc tttgaaaaaa ttaatgaagc gcgcagtcca ttactgccac
     6001 agctaggttt aggtgcagat tacacctata gcaacggcta ccgcgacgcg aacggcatca
     6061 actctaacgc gaccagtgcg tccctgcagt taactcaatc catttttgat atgtcgaaat
     6121 ggcgtgcgtt aacgctgcag gaaaaagcag cagggattca ggacgtcaca tatcagaccg
     6181 atcagcaaac cttgatcctc aacaccgcga ccgcttattt caacgtgttg aatgctattg
     6241 acgttctttc ctatacacag gcacaaaaag aagcgatcta ccgtcaatta gatcaaacca
     6301 cccaacgttt taacgtgggc ctggtagcga taaccgacgt gcagaacgcc cgcgcgcagt
     6361 acgataccgt gctggcgaac gaagtgaccg cacgtaataa ccttgataac gcggtagagc
     6421 agctgcgcca gatcaccggt aactactatc cagaactggc agcgctgaat gtcgaaaact
     6481 ttaaaaccga caaaccacag ccggttaacg cgctgctgaa agaagccgaa aaacgcaacc
     6541 tgtcgctgtt acaggcacgc ttgagccagg acctggcgcg cgagcaaatt cgccaggcgc
     6601 aggatggtca cttaccgacg ctggatttaa cggcttctac cgggatttct gacacctctt
     6661 atagcggttc gaaaactcgt ggtgccgctg gtacccagta tgacgacagc aatatgggcc
     6721 agaacaaagt gggcctgagc ttctcgctgc cgatttatca gggcggaatg gttaactcgc
     6781 aggtgaaaca ggcccagtac aactttgttg gtgccagcga gcaactggaa agcgcgcatc
     6841 gtagcgtcgt gcaaaccgta cgttcctcct tcaacaacat taatgcatct atcagtagca
     6901 ttaacgccta caaacaagcc gtagtttccg ctcaaagctc attagacgcg atggaagcgg
     6961 gctactcggt cggtacgcgt accattgttg atgtgttgga tgcaaccacc acgctgtaca
     7021 acgctaagca agagctggca aatgcgcgtt ataactacct gattaatcag ctgaatatta
     7081 agtcagccct gggtacgttg aacgagcagg atctgctggc actgaacaat gcgctgagca
     7141 aaccggtttc cactaatccg gaaaacgttg ccccgcaaac gccggaacag aatgctattg
     7201 ctgatggtta tgcgcctgat agcccggcac ccgtcgttca gcaaacatcc gcacgcacta
     7261 ccaccagtaa cggtcataac cctttccgta actgatgacg acgacggggc ttcggccccg
     7321 tctgaacgta aggcaacgta aagatacggg ttatctgccg cattcttccc ccttctcgct
     7381 tcaatttcga ccagccatcc tctattctga tgggtattta ccactggtcc cggaagacaa
     7441 aaatgaaacg gacaaaatcc atacgccacg catcgttccg caaaaactgg agcgcgcgcc
     7501 atctgacacc agtcgctctc gcggttgcca ctgtttttat gctggctggc tgtgaaaaga
     7561 gtgatgaaac agtgtctctc tatcaaaatg ctgacgactg ttcagctgca aacccaggca
     7621 aaagcgccga atgtaccacc gcgtacaaca atgcgctgaa agaagccgaa cgtactgcgc
     7681 cgaaatacgc cacccgtgaa gactgtgttg ctgaatttgg tgaaggtcag tgccagcagg
     7741 caccagccca ggctggcatg gcaccagaaa accaggcgca ggcccagcaa tccagcggga
     7801 gtttctggat gccgctgatg gccggttaca tgatggggcg tctgatgggc ggcggcgcgg
     7861 gatttgcaca gcagccgctg ttctcctcga aaaacccagc cagcccggct tacggtaaat
     7921 ataccgacgc gacgggtaaa aactatggcg cagcccagcc aggccgcacc atgaccgtac
     7981 cgaagacagc catggcacca aaaccggcga ccaccactac cgttacccgt ggcggttttg
     8041 gtgaatctgt tgccaaacaa agcactatgc agcgtagcgc aactggcacc tcttctcgtt
     8101 caatgggtgg ctgataccga tggaaagagt cagtattacc gagcgcccgg actggcgtga
     8161 gaaagcccac gaatacggtt tcaattttca caccatgtac ggcgagccgt actggtgtga
     8221 agatgcttac tacaagttga ccctcgccca ggttgaaaag ctggaagaag tcaccgccga
     8281 actgcaccag atgtgcctga aagtggtgga aaaagtgatc gccagcgatg agctgatgac
     8341 caaattccgc attccaaaac acacctggag ttttgtgcgt cagtcatggc tgactcacca
     8401 gccatcgctt tattcgcgtc ttgatctggc atgggatggc actggcgaac ctaaactgct
     8461 ggaaaataac gccgatacac caacgtcact atacgaggcg gcgttctttc agtggatctg
     8521 gctggaagat cagcttaacg ccggtaactt gccggagggc agcgaccagt ttaacagtct
     8581 gcaagaaaag ctaatcgatc gctttgtcga gttgcgtgaa cagtatggct tccagttgct
     8641 gcatctcacc tgctgtcgcg acacggtgga agatcgcgga accattcagt atttgcagga
     8701 ctgcgcgacg gaagctgaaa tcgctaccga gttcctctac attgacgata tcgggttagg
     8761 tgaaaaaggt cagttcacgg atttacagga tcaggtaatt tccaacctgt tcaagctgta
     8821 tccatgggaa tttatgttgc gtgagatgtt ctccaccaag ctggaggatg caggcgtacg
     8881 ctggctggaa ccggcgtgga agagcattat ctccaacaaa gcgcttctac cgctactgtg
     8941 ggagatgttc ccgaatcacc cgaacctgct gcccgcttat tttgcggaag atgatcatcc
     9001 gcaaatggaa aaatatgtgg ttaaaccgat cttctcccgt gaaggcgcaa acgtgtcgat
     9061 cattgagaac ggcaaaacca ttgaagcagc ggaaggtccg tatggcgaag aagggatgat
     9121 tgttcagcaa ttccacccgt taccgaaatt cggcgacagc tatatgctga ttggtagctg
     9181 gctggtgaac gatcaacctg ccggaattgg cattcgtgaa gaccgtgcgt tgatcaccca
     9241 ggatatgtct cggttttatc cacatatttt tattgagtaa tcaatgatgc cggatggcat
     9301 tcgccatccg gtaattgtta acctatctgc accgacagca tactcaggct gcccatttct
     9361 ataccatcga ccggaatggt aattggctcc tgcccatccc acgcacctaa gacatacaac
     9421 aacggcagat agtgctccgg cgttgggttc gataacgcgc caccttcatg gtcgaggtaa
     9481 ttcaccagag gatgctgctc taccgcccct tgccacgtca gattcgcttt cacatactca
     9541 ttaaacgaca tcgcccaagg atatggtgaa ctatcaccgt gccacttcac tgtacgcagg
     9601 ttatgcacca cgttgccact ggcgaccaac attattcctt catctcgcag cgctgccagt
     9661 ttgcgcccca tttcgaaatg ccaggcggca ggtttgctac tgtcgatact caactgcacc
     9721 atcgggatat cggcgtcagg atacatctta atcagcacgc cccaggaacc gtggtcaaag
     9781 ccccaggctt ctttatccag cgtcaccggg accggcgcta acagctcaac cagatgctgt
     9841 gccagcatag gcgaacccgg agcaggataa tgcgtatcgt acagcgcctg cgggaagcca
     9901 ccaaagtcat gaatcgtggg cggcgtctcc atcgcggtca ctcctgttcc acgggtaaac
     9961 cagtgagccg aaaccaccac aatcgcttgc gggcgtggca atgtcatccc caacttctgc
    10021 cagctgcggg tatacaaatt atcttccagc acgttcatcg gactaccgtg acctaaaaac
    10081 aatgctggca tacgtgttga agacatgatg atatccttaa ctaaaggtgt cattttgata
    10141 tcctcacaat acgcttgttc ggcggagtaa gaacccggat aacaatgatg atgatcatca
    10201 gttattttga cgatctgcct gaaagtgaag atttataagg agttgtcgat gtcagtacct
    10261 ctcattctga ccatactggc gggggcagcc acgtttattg gcgcgtttct cggcgttctc
    10321 gggcaaaaac cctcgaaccg cttactggcg ttttcgctag gatttgcggc ggggatcatg
    10381 ttgctcatct cattaatgga aatgcttcct gccgcactag cagctgaagg aatgtcgcct
    10441 gtgttgggtt atggaatgtt tatcttcggt ctgcttggct attttggcct ggaccgcatg
    10501 ttgccacatg cgcatccgca ggatttaatg caaaaatcgg tgcagccgtt gccaaaatcg
    10561 atcaagcgca cagccattct gctcactctc ggcatcagtc tgcataactt cccggaaggg
    10621 attgccacct ttgtcacggc gagcagcaac ctggagctgg gatttggcat cgcactggcc
    10681 gtcgctttgc acaatatccc tgaaggtctg gcagtggcag gcccggttta tgcggcaacg
    10741 gggtctaaac gcaccgcgat tctgtgggcg gggatttctg gactagcaga aattcttggt
    10801 ggtgtgctgg cgtggttaat cctcggtagc atgatttccc cggtggtcat ggcggcaatc
    10861 atggcggcgg ttgcaggaat tatggtggcg ctctcggttg atgaattaat gccgctcgcc
    10921 aaagagattg accctaataa taaccccagc tatggcgtac tatgtggaat gtcagtgatg
    10981 ggattcagtt tagtgctgct acaaacggcg ggaattggtt aaaaacaaca tcagaagttg
    11041 tccagcacat ggacaacttc tgccagcttc acgaaagcct tattctacct tttcggctgt
    11101 ctcttctctc gtactgttta agtatttgtt ccgcgcttca agcagtgcgt ctctgtcctg
    11161 gcgaaacgcc cgacaataac gtttcaaatg caggaaataa cccaccacaa ccagagcaat
    11221 caatacaatc cagtaccagg cgataaacat ccttttaccc ttaatattta acaatactaa
    11281 acatttagcg cataaatttc acatatcctt tttcggatat attcatatgg tcgcataata
    11341 acggaacaat tatatgattt tgatcataat gaaaaagaat gcctgatatt aatgaaattt
    11401 tctcatagaa agagaagcgg aattaacatc ttgcatagcg gggcaaaata aaaaccgaag
    11461 ccagctacaa cttcggtttc ttgattaagg cagtaattaa gcagcggttt tcagctggct
    11521 ttacgctcat gtgcctgacg gtatgccacc aggtcttcaa tagtcacgag cgccatattg
    11581 tgtttattgg caaactcaat gcactctggt gcacgcgcca tcgtgccatc gtcattagtc
    11641 agctcacaca gcacaccagc cggtttaaag cctgccagcg tcatcaaatc aatagtcgct
    11701 tcagtatgac cgccacgcgt cagtacacca cctgcctgcg cgcgaagtgg gaaaacgtgg
    11761 cccgggcgat tcagatcaga aggttttgcg ccatcggcaa tcgctgcgcg aacggtcgta
    11821 atacgatcag cggcagaaac accggtagtc acaccttcag ccgcttcaat ggtcacggta
    11881 aaaccggtgc cataggcgct ggtgttattt tctaccatca ttggcagatc aagttgttta
    11941 cggcgatctt cagtgatgca caggcaaaca ataccgctac cgtggcgaat ggtcagcgcc
    12001 atctgctcaa cggtcatggt ttctgccggg aagatcatat cgccttcgtt ttcacggtct
    12061 tcatcatcaa gcaccattac accgcgtcct tcacgcagcg cagccagtgc attttcaaca
    12121 cgttcgaaag gcgtaccaaa agaggaaagt agcgtctgat tcatggtaaa aaaacctcac
    12181 taaaattatg gttaccagaa tcagggcagt cttaggagtg gcggcatata gccaaaataa
    12241 cgtgagcggg tccatgcccg acagaatcgt tactctctcc catccggact ctaaccgtcg
    12301 gccccggaat tacaccggat ctgctgtcct ttgagttcgc acccaaagcg ctcgcgggct
    12361 ttcaactgag ttgatttacc gccggtgggg aatttcgccc cgccctgaga ataagcggat
    12421 tcactataac gctaatgatt agcggcagca acgcatagct tcacataatt ctggtttatg
    12481 acttaccctt atcgcactac aatggtactc aacacctatc agtacaggga aactacaatg
    12541 attgacccga aaaaaattga gcaaatcgct cgccaggttc acgaatcaat gcctaaagga
    12601 atcagggagt tcggggaaga tgtggagaaa aaaatccgcc aaaccctaca agctcagctg
    12661 acgcgcctcg accttgtaag ccgtgaagag ttcgacgtcc aaacgcaagt cctgttacgg
    12721 actcgtgaga aactggcgct gcttgaacaa agaatgagcg aactggaaaa tcgcagtact
    12781 gagattaaaa agcagccaga tccagagact ctccccccaa cgctgtaaac atttgtcttt
    12841 aatgcactgt aaattatgaa ttaatcctct aattta
//
LOCUS       DAZFQD010000148        12519 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.148, whole genome shotgun sequence.
ACCESSION   DAZFQD010000148 DAZFQD010000000
VERSION     DAZFQD010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12519
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.148"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..1260)
                     /gene="gltA"
                     /locus_tag="ACHKTW_004266"
     CDS             complement(<1..1260)
                     /gene="gltA"
                     /locus_tag="ACHKTW_004266"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HFO6465184.1"
                     /translation="MADTKAKLTLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSDGMKIARPRQLYTGYEKRD"
     gene            complement(1466..1573)
                     /locus_tag="ACHKTW_004267"
     CDS             complement(1466..1573)
                     /locus_tag="ACHKTW_004267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6465185.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            1954..2358
                     /gene="sdhC"
                     /locus_tag="ACHKTW_004268"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             1954..2358
                     /gene="sdhC"
                     /locus_tag="ACHKTW_004268"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0045273 - respiratory chain complex II
                     (succinate dehydrogenase) [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HFO6465186.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILLWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            2352..2699
                     /gene="sdhD"
                     /locus_tag="ACHKTW_004269"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             2352..2699
                     /gene="sdhD"
                     /locus_tag="ACHKTW_004269"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HFO6465187.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            2699..4465
                     /gene="sdhA"
                     /locus_tag="ACHKTW_004270"
     CDS             2699..4465
                     /gene="sdhA"
                     /locus_tag="ACHKTW_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HFO6465188.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALRDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            4481..5197
                     /gene="sdhB"
                     /locus_tag="ACHKTW_004271"
     CDS             4481..5197
                     /gene="sdhB"
                     /locus_tag="ACHKTW_004271"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HFO6465189.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            5498..8299
                     /gene="sucA"
                     /locus_tag="ACHKTW_004272"
     CDS             5498..8299
                     /gene="sucA"
                     /locus_tag="ACHKTW_004272"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HFO6465190.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELLEAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPSSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            8314..9531
                     /gene="odhB"
                     /locus_tag="ACHKTW_004273"
     CDS             8314..9531
                     /gene="odhB"
                     /locus_tag="ACHKTW_004273"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HFO6465191.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAADPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEDPTRLLLDV"
     gene            9625..10791
                     /gene="sucC"
                     /locus_tag="ACHKTW_004274"
     CDS             9625..10791
                     /gene="sucC"
                     /locus_tag="ACHKTW_004274"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HFO6465192.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            10791..11660
                     /gene="sucD"
                     /locus_tag="ACHKTW_004275"
     CDS             10791..11660
                     /gene="sucD"
                     /locus_tag="ACHKTW_004275"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HFO6465193.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGATASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(11764..12486)
                     /gene="mngR"
                     /locus_tag="ACHKTW_004276"
     CDS             complement(11764..12486)
                     /gene="mngR"
                     /locus_tag="ACHKTW_004276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HFO6465194.1"
                     /translation="MGHKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVDTHSEVLIF
                     EVIPADDFLQQQLQITAQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
BASE COUNT         2996 a         3201 c         3408 g         2914 t
ORIGIN      
        1 gtcgcgtttt tcatatcctg tatacagctg acgcggacgg gcaatcttca taccgtcact
       61 gtgcatttcg ctccagtggg cgatccagcc aacggtacgt gccattgcga aaatgacggt
      121 gaacatggaa gacggaatac ccatcgcttt caggatgata ccagagtaga aatcgacgtt
      181 cgggtacagt ttcttctcga taaagtacgg gtcgttcagc gcgatgtttt ccagctccat
      241 agccacttcc agcaggtcat ctttggtgcc cagctctttc agaacttcat ggcaggtttc
      301 acgcattacg gtggcgcgcg ggtcgtaatt tttgtacacg cggtgaccga agcccatcag
      361 gcggaaagaa tcatttttat ctttcgcacg acgaacaaat tccggaatgt gtttaacgga
      421 gctaatttct tccagcattt tcagcgccgc ttcgttagca ccaccgtgcg caggtcccca
      481 cagtgaagca atacctgctg cgatacaggc aaacgggttc gcacccgaag agccagcggt
      541 acgcacggtg gaggtagagg cgttctgttc atggtcagcg tgcaggatca gaatacggtc
      601 catagcacgt tccagaatcg gattaacttc atacggttcg cacggcgtgg agaacatcat
      661 attcaggaag ttaccggcat aggagagatc gttgcgcgga taaacaaatg gctgaccaat
      721 ggaatacttg taacacatcg cggccatggt cggcattttc gacagcaggc ggaacgcggc
      781 aatttcacga tgacgaggat tgttaacatc cagcgagtcg tgatagaacg ccgccagcgc
      841 gccggtaata ccacacatga ctgccattgg atgtgagtcg cgacggaaag cgtggaacag
      901 acgggtaatc tgctcgtgga tcatggtatg acgggtcacc gtagttttaa attcgtcata
      961 ctgttcctga gtcggttttt caccattcag caggatgtaa caaacttcca ggtagttaga
     1021 atcggtcgcc agctgatcga tcgggaaacc gcggtgcagc aaaatacctt catcaccatc
     1081 aataaaagta attttagatt cgcaggatgc ggttgaagtg aagcctgggt caaaggtgaa
     1141 cacacctttt gaaccgagag tacggatatc aataacatct tgacccagcg tgcctttcag
     1201 cacatccagt tcaacagctg tgtccccgtt gagggtgagt tttgcttttg tatcagccat
     1261 ttaaggtctc cttagcgcct tattacgtaa gactgccgga acttaaattt gccttcgcac
     1321 atcaacctgg ctttacccgt ttgttatttg gctcgccgct ctgtgaaaga ggggaaaacc
     1381 tgggtacaga gctctgggcg cttgcaggta aaggatccat tgatgacgaa taaatggcga
     1441 atcaagtact tagcaatccg aattattaaa cttgtctacc actaataact gtcccgaatg
     1501 aattggtcaa tacttccaca ctgttacata agttaatctt aggtgaaata ccgacttcat
     1561 aacttttacg cattatatgc ttttcctggt aatgtttgta acaactttgt tgaatgattg
     1621 tcaaattaga tgattaaaaa ttaaataaat gttgttatcg tgacctggat cactgttcag
     1681 gataaaaccc gacaaactat atgtaggtta attgtaatga ttttgtgaac agcctatact
     1741 gccgccaggt ctccggaaca ccctgcaatc ccgagccacc cagcgttgta acgtgtcgtt
     1801 ttcgcatctg gaagcagtgt tttgcatgac gcgcagttat agaaaggacg ctgtctgacc
     1861 cgcaagcaga ccggaggaag gaaatcccga cgtctccagg taacagaaag ttaacctctg
     1921 tgcccgtagt ccccagggaa taataagaac agcatgtggg cgttattcat gataagaaat
     1981 gtgaaaaaac aaagacctgt taatctggac ctacagacca tccggttccc catcacggcg
     2041 atagcgtcca ttctccatcg cgtttccggt gtgatcacct ttgttgcagt gggcatcctg
     2101 ctgtggcttc tgggtaccag cctctcttcc cctgaaggtt tcgagcaagc ttccgcgatt
     2161 atgggcagct tcttcgtcaa atttatcatg tggggcatcc ttaccgctct ggcgtatcac
     2221 gtcgtcgtag gtattcgcca catgatgatg gattttggct atctggaaga aacattcgaa
     2281 gcgggtaaac gctccgccaa aatctccttt gttattactg tcgtgctttc acttctcgca
     2341 ggagtcctcg tatggtaagc aacgcctccg cattaggacg caatggcgta catgatttca
     2401 tcctcgttcg cgctaccgct atcgtcctga cgctctacat catttatatg gtcggttttt
     2461 tcgccaccag tggcgaactg acatatgaag tctggatcgg tttcttcgcc tctgcgttca
     2521 ccaaagtgtt caccctgctg gcgctgtttt ctatcttgat tcatgcctgg atcggcatgt
     2581 ggcaggtgtt gaccgactac gttaaaccgc tggcattgcg cctgatgctg caactggtga
     2641 ttgtcgttgc actggtggtt tacgtgattt atggattcgt tgtggtgtgg ggtgtgtgat
     2701 gaaattgcca gtcagagaat ttgatgcagt tgtgattggt gccggtggcg caggtatgcg
     2761 cgcggcgctg caaatttccc agagcggcca gacctgtgcg ctgctctcta aagtcttccc
     2821 gacccgttcc cataccgttt ctgcgcaagg cggcattacc gttgcgctgg gtaataccca
     2881 tgaagataac tgggaatggc atatgtacga caccgtaaaa gggtcggact atatcggtga
     2941 ccaggacgcg attgaatata tgtgtaaaac cgggccggaa gcgattctgg aactcgaaca
     3001 catgggcctg ccgttctcgc gtcttgatga tggtcgtatc tatcaacgtc cgtttggcgg
     3061 tcagtcgaaa aacttcggcg gcgagcaggc ggcacgcact gcggcggcgg ctgaccgtac
     3121 cggtcacgca ctgttgcaca cgctttatca gcagaacctg aaaaaccaca ccaccatttt
     3181 ctccgagtgg tatgcgctgg atctggtgaa aaaccaggat ggcgcggtgg tgggttgtac
     3241 tgcactgtgc atcgaaactg gtgaagtggt ttatttcaaa gcccgcgcta ccgtgctggc
     3301 gactggcggg gcagggcgta tttatcagtc caccactaac gcccacatta acactggcga
     3361 cggcgtcggc atggctatcc gcgcaggcgt accggtgcag gatatggaaa tgtggcagtt
     3421 ccacccgacc ggtattgccg gtgcgggcgt actggtcacc gaaggttgcc gtggtgaagg
     3481 cggttatctg ctgaacaaac atggcgaacg ctttatggag cgttatgcgc cgaacgccaa
     3541 agacctggcg ggccgtgatg tggtggcgcg ttccatcatg atcgaaatcc gtgaaggtcg
     3601 cggctgtgat ggtccgtggg ggccacacgc gaaactgaaa ctcgaccacc tgggtaaaga
     3661 agttctcgaa tcccgcctgc cgggtatcct ggaactctcc cgtaccttcg ctcacgttga
     3721 tccggtgaaa gagccgattc cggttatccc aacctgtcac tacatgatgg gcggtattcc
     3781 gaccaaagtg accggtcagg cgctgacggt gaacgagaaa ggcgaagatg tggttgttcc
     3841 gggactgttt gccgttggtg aaatcgcttg tgtatcggta cacggcgcta accgtctggg
     3901 cggcaactcg ctgctggacc tggtggtctt tggtcgtgca gcgggtctgc atctgcaaga
     3961 gtctatcgcc gaacagggcg cattgcgcga tgccagcgag tctgatgttg aagcatctct
     4021 ggatcgtctg aaccgctgga acaataatcg taacggtgaa gatccggtgg cgattcgtaa
     4081 agcgctgcaa gaatgtatgc agcataactt ctcggtcttc cgtgaaggtg atgcgatggc
     4141 gaaagggctt gagcagttga aagtgatccg cgagcgtctg aaaaatgccc gtctggatga
     4201 cacttccagc gagttcaaca cccagcgcgt tgagtgcctg gaactggata acctgatgga
     4261 aacggcgtat gcaacggctg tttctgccaa cttccgtacc gaaagccgtg gcgcacatag
     4321 ccgcttcgac ttcccggatc gtgatgatga aaactggctg tgccactccc tgtatctgcc
     4381 tgagtcggaa tccatgacgc gccgaagcgt caacatggaa ccgaaactgc gcccggcatt
     4441 cccgccgaag attcgtactt actaatgcgg agacaggaaa atgagactcg agttttcaat
     4501 ttatcgctat aacccggatg ttgatgatgc tccgcgtatg caggattaca ccctggaagc
     4561 ggaagaaggt cgcgacatga tgctgctgga tgcgcttatc cagctgaaag agaaagatcc
     4621 cagcctgtcg ttccgccgct cctgccgtga aggtgtgtgc ggttccgacg gtctgaacat
     4681 gaacggcaag aatggtctgg cctgtattac cccgatttcg gcactcaacc agccgggcaa
     4741 gaagattgtg attcgcccgc tgccaggttt accggtgatc cgcgatttgg tggtagacat
     4801 gggacaattc tatgcgcaat atgagaaaat taagccttac ctgttgaata atggacaaaa
     4861 tccgccagct cgcgagcatt tacagatgcc agagcagcgc gaaaaactcg acgggctgta
     4921 tgaatgtatt ctctgcgcat gttgttcaac ctcttgtccg tctttctggt ggaatcccga
     4981 taagtttatc ggcccggcag gcttgttagc ggcatatcgt ttcctgatcg atagccgtga
     5041 taccgagact gacagccgcc tcgacggttt gagcgatgca ttcagcgtat tccgctgtca
     5101 cagcatcatg aactgcgtca gtgtatgtcc gaaggggctg aacccgacgc gcgccatcgg
     5161 ccatattaag tcgatgttgt tgcaacgtaa tgcgtaaacc gtaggcctga taagacgcgc
     5221 aagcgtcgca tcaggcaacc agtgccggat gcggcgtgaa cgccttatcc ggcctacaag
     5281 tcattacccg taggcctgat aagcgcagcg catcaggcgt aacaaagaaa tgcaggaaat
     5341 ctttaaaaac tgcccctgac actaagacag tttttaaagg ttccttcgcg agccactaca
     5401 tagacaagag ctcgcaagtg aaccccggca cgcacatcac tgtgcgtggt agtatccacg
     5461 gcgaagtaag cataaaaaag atgcttaagg gatcacgatg cagaacagcg ctttgaaagc
     5521 ctggttggac tcttcttacc tctctggcgc aaaccagagc tggatagaac agctctatga
     5581 agacttctta accgatcctg actcggttga cgctaactgg cgttcgacgt tccagcagtt
     5641 acctggtacg ggagtcaaac cggatcaatt ccactctcaa acgcgtgaat atttccgccg
     5701 cctggcgaaa gacgcttcac gttactcttc aacgatctcc gaccctgaca ccaatgtgaa
     5761 gcaggttaaa gtcctgcagc tcattaacgc ataccgcttc cgtggtcacc agcatgcgaa
     5821 tctcgatccg ctgggactgt ggcagcaaga taaagtggcc gatctggatc cgtctttcca
     5881 cgatctgacc gaagcagact tccaggaaac cttcaacgtc ggttcatttg ccagcggcaa
     5941 agaaaccatg aaactcggcg agctgctgga agccctcaag caaacctact gtggcccgat
     6001 tggtgccgag tatatgcaca ttaccagcac cgaagaaaaa cgctggatcc aacagcgtat
     6061 cgagtctggt cgcgcgactt tcaatagcga agagaaaaaa cgcttcttaa gcgaactgac
     6121 cgccgctgaa ggccttgaac gttacctcgg cgcaaaattc cctggcgcaa aacgcttctc
     6181 gctggaaggc ggtgacgcgt taatcccgat gcttaaagag atgatccgcc acgctggcaa
     6241 cagcggcacc cgcgaagtgg ttcttgggat ggcgcaccgt ggtcgtctga acgtgctggt
     6301 gaacgtgctg ggtaaaaaac cgcaagactt gttcgacgag tttgccggta aacataaaga
     6361 acacctcggc acgggtgacg tgaaatacca catgggcttc tcgtctgact tccagaccga
     6421 tggcggcctg gtgcacctgg cgctggcgtt taacccgtct caccttgaga ttgtaagccc
     6481 ggtagttatc ggttctgttc gtgcccgtct ggacagactt gatgagccga gcagcaacaa
     6541 agtgctgcca atcaccatcc acggtgacgc cgcagtgacc gggcagggcg tggttcagga
     6601 aactctgaac atgtcgaaag cgcgtggtta tgaagttggc ggtacggtac gtatcgttat
     6661 caacaaccag gttggtttca ccacctctaa tccgctggat gctcgttcta caccgtactg
     6721 tactgatatc ggtaagatgg ttcaggcacc gattttccac gttaacgcgg atgatccgga
     6781 agccgttgcc tttgtgaccc gtctggcgct cgatttccgt aacaccttta aacgtgatgt
     6841 cttcatcgac ctggtgtgct accgccgtca cggccacaac gaagccgacg agccgagcgc
     6901 aacccagccg ctgatgtatc agaaaatcaa aaaacatccg acaccgcgca aaatctacgc
     6961 tgacaagctg gagcaggaaa aagtcgcgac gctggaagat gccaccgaga tggttaacct
     7021 gtaccgcgat gcgctggatg ctggcgattg cgtagtggca gagtggcgtc cgatgaacat
     7081 gcactctttc acctggtcgc cgtacctcaa ccacgaatgg gacgaagagt acccgaacaa
     7141 agttgagatg aagcgcctgc aggagctggc gaaacgcatc agcacggtgc cggaagcagt
     7201 tgaaatgcag tctcgcgttg ccaagattta tggcgatcgc caggcgatgg ctgccggtga
     7261 gaaactgttc gactggggcg gtgcggaaaa cctcgcttac gccacgctgg ttgacgaagg
     7321 cattccggtt cgcctgtcgg gtgaagactc cggtcgcggt accttcttcc accgccacgc
     7381 ggtgatccac aaccagtcta acggttccac ttacacgccg ctgcaacata ttcataacgg
     7441 ccagggcgcg ttccgtgtct gggactccgt tctgtctgaa gaagcagtgc tggcgtttga
     7501 atacggttat gccaccgcag aaccacgcac tctgactatc tgggaagcgc agttcggtga
     7561 cttcgccaac ggtgcgcagg tggttatcga ccagttcatc tcctctggcg agcagaaatg
     7621 gggccggatg tgtggtctgg tgatgttgct gccgcacggt tacgaagggc aggggccgga
     7681 gcactcctcc gcgcgtctgg aacgttatct gcaactttgt gctgagcaaa acatgcaggt
     7741 ttgcgtaccg tctaccccgg cacaggttta ccacatgctg cgtcgtcagg cgctgcgcgg
     7801 gatgcgtcgt ccgctggtcg tgatgtcgcc gaaatccctg ctgcgtcatc cgctggcggt
     7861 ttccagcctc gaagaactgg cgaacggcac cttcctgcca gccatcggtg aaatcgacga
     7921 gcttgatccg aagggcgtga agcgcgtagt aatgtgttct ggtaaggttt attacgacct
     7981 gctggaacag cgtcgtaaga acaatcaaca cgatgtcgcc attgtgcgta tcgagcaact
     8041 ctacccgttc ccgcataaag cgatgcagga agtgttgcag cagtttgctc acgtcaagga
     8101 ttttgtctgg tgccaggaag agccgctcaa ccagggcgca tggtactgca gccagcatca
     8161 tttccgtgaa gtgattccgt ttggggcttc tctgcgttat gcaggccgcc cggcctccgc
     8221 ctctccggcg gtagggtata tgtccgttca ccagaaacag caacaagatc tggttaatga
     8281 cgcgctgaac gtcgaataaa taaaggatac acaatgagta gcgtagatat tctggtccct
     8341 gacctgcctg aatccgtagc cgatgccacc gtcgcaacct ggcataaaaa acccggcgac
     8401 gcagtcgtac gtgatgaagt gctggtagaa atcgaaactg acaaagtggt actggaagta
     8461 ccggcatcag cagacggcat tctggatgcg gttctggaag atgaaggtac aacggtaacg
     8521 tctcgtcaga tccttggtcg cctgcgtgaa ggcaacagcg ccggtaaaga aaccagcgcc
     8581 aaatctgaag agaaagcgtc cactccggcg caacgccagc aggcgtctct ggaagagcaa
     8641 aacaacgatg cgttaagccc ggcgatccgt cgcctgctgg ctgaacacaa tctcgacgcc
     8701 agcgccatta aaggcaccgg tgtgggtggt cgtctgactc gtgaagatgt ggaaaaacat
     8761 ctggcgaaag ccccggcgaa agagtctgct ccggcagcgg ctgatccggc ggcgcaaccg
     8821 gctctggctg cacgtagtga aaaacgtgtc ccgatgactc gcctgcgtaa gcgtgtggca
     8881 gagcgtctgc tggaagcgaa aaactccacc gccatgctga ccacgttcaa cgaagtcaac
     8941 atgaagccga ttatggatct gcgtaagcag tacggtgaag cgtttgaaaa acgccacggc
     9001 atccgtctgg gctttatgtc cttctacgtg aaagcggtgg ttgaagccct gaaacgttac
     9061 ccggaagtga acgcgtctat cgacggcgat gacgtggttt accacaacta tttcgacgtc
     9121 agcatggcgg tttctacgcc gcgcggcctg gtgacaccgg tactgcgtga tgtcgatacc
     9181 ctcggcatgg cagacatcga gaagaaaatc aaagagctgg cagtcaaagg ccgtgacggc
     9241 aagctgaccg ttgaagatct gaccggtggt aacttcacca tcaccaacgg tggtgtgttc
     9301 ggttccctga tgtctacgcc gatcatcaac ccgccgcaga gcgcaattct gggtatgcac
     9361 gctatcaaag atcgtccgat ggcggtgaat ggtcaggttg agatcctgcc gatgatgtac
     9421 ctggcgctgt cctacgatca ccgtctgatc gatggtcgcg aatccgtggg cttcctggta
     9481 acgatcaaag agttgctgga agatccgacg cgtctgctgc tggacgtgta gtagtttaag
     9541 cttcacctgc actgtagacc gacgtggata gggtgtcggt ctacggttta aaagataacg
     9601 attactgaag gatggacaga acacatgaac ttacatgaat atcaggcaaa acaacttttt
     9661 gcccgctatg gcttaccagc accggtgggt tatgcctgta ctactccgcg cgaagcagaa
     9721 gaagccgctt caaaaatcgg tgccggcccg tgggtagtga aatgtcaggt tcacgctggt
     9781 ggccgcggta aagcgggcgg tgtgaaagtt gtaaacagca aagaagacat ccgtgctttt
     9841 gcagaaaact ggctcggcaa gcgtctggta acgtatcaaa cagatgccaa tggccaaccg
     9901 gttaaccaga ttctggttga agcagcgacc gatatcgcta aagagctgta tctcggtgcc
     9961 gttgtagacc gtagttcccg tcgcgtggtc tttatggcct ccaccgaagg cggcgtggaa
    10021 atcgaaaaag tggcggaaga aactccgcac ctgatccata aagttgcgct tgatccgctg
    10081 acaggcccga tgccgtatca gggacgcgag ctggcgttca aactgggtct ggaaggtaaa
    10141 ctggttcagc agttcaccaa aatcttcatg ggcctggcga ccattttcct ggagcgcgac
    10201 ctggcgttga tcgaaatcaa cccgctggtc atcaccaaac agggcgatct gatttgcctc
    10261 gacggcaaac tgggcgctga cggcaacgca ctgttccgcc agcctgatct gcgcgaaatg
    10321 cgtgaccagt cgcaggaaga tccgcgtgaa gcacaggctg cacagtggga actgaactac
    10381 gttgcgctgg acggtaacat cggttgtatg gttaacggcg caggtctggc gatgggtacg
    10441 atggacatcg ttaaactgca cggcggcgaa ccggctaact tccttgacgt tggcggcggc
    10501 gcaaccaaag aacgtgtaac cgaagcgttc aaaatcatcc tctctgacga caaagtgaaa
    10561 gccgttctgg ttaacatctt cggcggtatc gttcgttgcg acctgatcgc tgacggtatc
    10621 atcggcgcgg tagcagaagt gggtgttaac gtaccggtcg tggtacgtct ggaaggtaac
    10681 aacgccgaac tcggcgcgaa gaaactggct gacagcggcc tgaatattat tgcagcaaaa
    10741 ggtctgacgg atgcagctca gcaggttgtt gccgcagtgg aggggaaata atgtccattt
    10801 taattgataa aaacaccaag gttatctgcc agggctttac tggtagccag gggactttcc
    10861 actcagaaca ggccattgca tacggcacta aaatggttgg cggcgtaacc ccaggtaaag
    10921 gcggtactac ccacctcggc ctgccggtgt tcaacaccgt gcgtgaagct gttgctgcta
    10981 ctggcgctac cgcttctgtt atctacgtac cagcaccgtt ctgcaaagac tccattctgg
    11041 aagctattga tgcaggcatc aaactgatta tcaccatcac tgaaggcatc ccgacgctgg
    11101 atatgctgac tgtgaaagtg aaactggacg aagccggcgt tcgtatgatc ggcccgaact
    11161 gcccgggcgt tatcactccg ggtgaatgca aaatcggtat ccagccgggt cacattcaca
    11221 aaccgggtaa agtgggtatc gtttcccgtt ccggtacact gacctatgaa gcggttaaac
    11281 agaccacgga ttacggtttc ggtcagtcga cctgtgttgg tatcggcggt gacccgatcc
    11341 ctggctctaa ctttatcgac attctcgaaa tgttcgaaaa agatccgcag accgaagcga
    11401 tcgtaatgat cggtgagatc ggcggtagcg ctgaagaaga agcggcagcg tacatcaaag
    11461 agcatgttac caagccagtt gtgggttaca tcgctggtgt gactgcgccg aaaggcaaac
    11521 gtatgggcca cgcgggtgcc atcattgccg gtgggaaagg gactgcggat gagaaattcg
    11581 ctgctctgga agccgcaggc gtgaaaaccg ttcgcagcct ggcggatatc ggtgaagcac
    11641 tgaaaactgt tctgaaataa atatctgtaa taagaaatag ccctcgccgc ttccctctac
    11701 aggaatggcg aagggctgtc ggtttcgaca tggttggcca tcgtatgatg gccttttttg
    11761 tgcttatcgc gatgattttc gttgcgctat cagggtaaat ttatagtcat cggtattaaa
    11821 agcgttgcgg ctatattcaa acacccgacc atcaactaaa tatccacgcg atactttttc
    11881 aagaatcggc tttgtctggc tgatattaag cagacggctc atctcttcgg ttggcatcag
    11941 aggaatgatt tcctgttcgc tacgatcgat aaccattttc ttcacttctt caataaagtg
    12001 atattttgaa ttttccatga cctgccaggt gagatccggg aacaacgcaa gcggcatcca
    12061 ggtttcttcc agcgccattg gcttttgctt gcgatagcgc acgcgcttca catgccacac
    12121 acgatcctgc gcggtgattt gtagctgttg ctgaagaaaa tcgtcagccg gaatcacttc
    12181 aaatatcagg acttcactgt gtgtatcgac gtgacggtcc gacagttttt catcaaaact
    12241 ggttaactga aaaatatcgt aattgacccg ctcttctttg acgtaagtcc cgctgccctg
    12301 aatgctttcg aggatctgtt gctcgactaa ctggcgcaaa gcctgacgca ccgtaacccg
    12361 gctgacgcca aactctgttt gtaatgccga ttcggtaggt aacgcatcgc caggtttaag
    12421 ctcgccacgc gcaatttgtt cacgaatgcg atcggcaatc tgccggtata agggcttgtg
    12481 tcccattttt agtatctcat taatacgaat ttagccatt
//
LOCUS       DAZFQD010000149        12286 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.149, whole genome shotgun sequence.
ACCESSION   DAZFQD010000149 DAZFQD010000000
VERSION     DAZFQD010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12286
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.149"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..1171)
                     /gene="ydcO"
                     /locus_tag="ACHKTW_004277"
     CDS             complement(<1..1171)
                     /gene="ydcO"
                     /locus_tag="ACHKTW_004277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BenE family transporter YdcO"
                     /protein_id="HFO6465195.1"
                     /translation="MRLFSIPPPTLLAGFLAVLIGYASSAAIIWQAAIVAGATTAQIS
                     GWMTALGLAMGVSTLTLTLWYRVPVLTAWSTPGAALLVTGLQGLTLNEAIGVFIVTNA
                     LIVLCGITGLFARLMRIIPHSLAAAMLAGILLRFGLQAFASLDGQFTLCGSMLLVWLA
                     TKAVAPRYAVIAAMIIGIVIVIAQGDVVTTDVVFKPVLPTYITPDFSFAHSLSVALPL
                     FLVTMASQNAPGIAAMKAAGYSAPVSPLIVFTGLLALVFSPFGVYSVGIAAITAAICQ
                     SPEAHPDKDQRWLAAAVAGIFYLIAGLFGSAITGMMAALPVSWIQMLAGLALLSTISG
                     SLYQALHNERERDAAVVAFLVTASGLTLVGIGSAFWGLIAGGVCYVVLNLIADRNR"
     gene            1263..1799
                     /gene="sutR"
                     /locus_tag="ACHKTW_004278"
     CDS             1263..1799
                     /gene="sutR"
                     /locus_tag="ACHKTW_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SutR"
                     /protein_id="HFO6465196.1"
                     /translation="MENLARFLSTTLKQLRQQRGWSLSRLAEATGVSKAMLGQIERNE
                     SSPTVATLWKIATGLNVPFSTFISPPQSATPSVYDPQQQAMVITSLFPYDPQLCFEHF
                     SIQMASGAISESTPHEKGVIEHVVVIDGQLDLCVDGEWQTLNCGEGVRFAADVTHIYR
                     NGGEQTVHFHSLIHYPRS"
     gene            1872..3833
                     /gene="rlhA"
                     /locus_tag="ACHKTW_004279"
     CDS             1872..3833
                     /gene="rlhA"
                     /locus_tag="ACHKTW_004279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA 5-hydroxycytidine C2501 synthase"
                     /protein_id="HFO6465197.1"
                     /translation="MTVSSHRLELLSPARDAAIAREAILHGADAVYIGGPGFGARHNA
                     SNSLKDIAELVPFAHRYGAKIFVTLNTILHDDELEPAQRLITDLYQTGVDALIVQDMG
                     ILELDIPPIELHASTQCDIRTVEKAKFLSDVGFTQIVLARELNLDQIRAIHQATDATI
                     EFFIHGALCVAYSGQCYISHAQTGRSANRGDCSQACRLPYTLKDDQGRVVSYEKHLLS
                     MKDNDQTANLGALIDAGVRSFKIEGRYKDMSYVKNITAHYRQMLDAIIEERGDLARAS
                     SGRTEHFFVPSTEKTFHRGSTDYFVNARKGDIGAFDSPKFIGLPVGEVLKVAKDHLDV
                     AVTEPLANGDGLNVLIKREVVGFRANTVEKTGENQYRVWPNEMPADLHKIRPHHPLNR
                     NLDHNWQQALTKTSSERRVAVDIELGGWQEQLILTLTSEEGVSITHTLDGQFDEANNA
                     EKAMNNLKDGLAKLGQTLYYARDVQINLPGALFVPNSLLNQFRREAADMLDAARLASY
                     QRGSRKPVADPAPVYPQTHLSFLANVYNQKAREFYHRYGVQLIDAAYEAHEEKGEVPV
                     MITKHCLRFAFNLCPKQAKGNIKSWKATPMQLVNGDEVLTLKFDCRPCEMHVIGKIKN
                     HILKMPLPGSVVASVSPDELLKTLFKRKG"
     gene            complement(3925..4155)
                     /gene="yncJ"
                     /locus_tag="ACHKTW_004280"
     CDS             complement(3925..4155)
                     /gene="yncJ"
                     /locus_tag="ACHKTW_004280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncJ family protein"
                     /protein_id="HFO6465198.1"
                     /translation="MFTKALSVVLLTCALFSGQLMAGHKGHEFVWVKNVDHQLRHEAD
                     SDELRAVAEESAEGLREHFYWQKSRKPEAGQR"
     gene            4377..4553
                     /gene="hicA"
                     /locus_tag="ACHKTW_004281"
     CDS             4377..4553
                     /gene="hicA"
                     /locus_tag="ACHKTW_004281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin HicA"
                     /protein_id="HFO6465199.1"
                     /translation="MKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIK
                     EPLRKAILKQLGLS"
     gene            4599..5015
                     /gene="hicB"
                     /locus_tag="ACHKTW_004282"
     CDS             4599..5015
                     /gene="hicB"
                     /locus_tag="ACHKTW_004282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HicB"
                     /protein_id="HFO6465200.1"
                     /translation="MRYPVTLTPAPEGGYMVSFVEIPEALTQGETVAEAMEAAKDALL
                     TAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKVLLLNAFLQSEITQQELARRIGK
                     PKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV"
     gene            5094..6500
                     /gene="ydcR"
                     /locus_tag="ACHKTW_004283"
     CDS             5094..6500
                     /gene="ydcR"
                     /locus_tag="ACHKTW_004283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFO6465201.1"
                     /translation="MKKYQQLAEQLREQIASGIWQPGDRLPSLRDQVALSGMSFMTIS
                     HAYQLLESLGYIIARPQSGYYVAPQAIKMPKAPVIPVTRDEAVDINTYIFDMLQASRD
                     PSVVPFASAFPDPRLFPLQQLNRSLAQVSKTATAMSVIENLPPGNAELRQAIARRYAL
                     QGITISPDEIVITAGALEALNLSLQAVTEPGDWVIVENPCFYGALQALERLRLKALSV
                     ATDVKEGIDLQALELALQDYPVKACWLMTNSQNPLGFTLTPQKKAQLVALLNQYNVTL
                     IEDDVYSELYFGREKPLPAKAWDRHDGVLHCSSFSKFLVPGFRIGWVAAGKHARKIQR
                     LQLMSTLSTSSPMQLALVDYLSTRRYDAHLRRLRRQLAERKQRAWQALLRYLPAEVKI
                     HHSDSGYFLWLELPEPLDAGELSLAALTHHISIAPGKMFSTGENWSRFFRFNTAWQWG
                     EREEQAVKQLGKLIQERL"
     gene            6745..7890
                     /gene="ydcS"
                     /locus_tag="ACHKTW_004284"
     CDS             6745..7890
                     /gene="ydcS"
                     /locus_tag="ACHKTW_004284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter substrate-binding
                     protein YdcS"
                     /protein_id="HFO6465202.1"
                     /translation="MSKTFARSSLCALTMTIMTAHAAEPPTNLDKPEGRLDIIAWPGY
                     IERGQTDKQYDWVTQFEKETGCAVNVKTAATSDEMVSLMTKGGYDLVTASGDASLRLI
                     MGKRVQPINTALIPNWKTLDPRVVKGDWFNVGGKVYGTPYQWGPNLLMYNTKTFPTPP
                     DSWQVVFVEQNLPDGKSNKGRVQAYDGPIYIADAALFVKATQPQLGISDPYQLTEEQY
                     QAVLKVLRAQHSLIHRYWHDTTVQMSDFKNEGVVASSAWPYQANALKAEGQPVATVFP
                     KEGVTGWADTTMLHSEAKHPVCAYKWMNWSLTPKVQGDVAAWFGSLPVVPEGCKASPL
                     LGEKGCETNGFNYFDKIAFWKTPIAEGGKFVPYSRWTQDYIAIMGGR"
     gene            7908..8921
                     /gene="ydcT"
                     /locus_tag="ACHKTW_004285"
     CDS             7908..8921
                     /gene="ydcT"
                     /locus_tag="ACHKTW_004285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO6465203.1"
                     /translation="MTYAVEFDNVSRLYGDVRAVDGVSIAIKDGEFFSMLGPSGSGKT
                     TCLRLIAGFEQLSGGAISIFGKPASNLPPWERDVNTVFQDYALFPHMSILDNVAYGLM
                     VKGVNKKQRHAMAQEALEKVALGFVHQRKPSQLSGGQRQRVAIARALVNEPRVLLLDE
                     PLGALDLKLREQMQLELKKLQQSLGITFIFVTHDQGEALSMSDRVAVFNNGRIEQVDS
                     PRDLYMRPRTPFVAGFVGTSNVFDGLMAEKLCGMTGSFALRPEHIRLNTPGELQANGT
                     IQAVQYQGAATRFELKLNGGEKLLVSQANMTGEELPATLTPGQQVMVSWSRDVMVPLV
                     EER"
     gene            8922..9863
                     /gene="ydcU"
                     /locus_tag="ACHKTW_004286"
     CDS             8922..9863
                     /gene="ydcU"
                     /locus_tag="ACHKTW_004286"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO6465204.1"
                     /translation="MAMNVLQSPSRPGLGKVSGFFWRNPGLGLFLLLLGPLMWFGIVY
                     FGSLLTLLWQGFYTFDDFTMSVTPELTLANIRALFNPANYDIILRTLTMAVAVTIASA
                     ILAFPMAWYMARYTSGKMKAFFYIAVMLPMWASYIVKAYAWTLLLAKDGVAQWFLQHL
                     GLEPLLTAFLTLPAVGGNTLSTSGLGRFLVFLYIWLPFMILPVQAALERLPPSLLQAS
                     ADLGARPRQTFRYVVLPLAIPGIAAGSIFTFSLTLGDFIVPQLVGPPGYFIGNMVYSQ
                     QGAIGNMPMAAAFTLVPIVLIALYLAFVKRLGAFDAL"
     gene            9853..10647
                     /gene="ydcV"
                     /locus_tag="ACHKTW_004287"
     CDS             9853..10647
                     /gene="ydcV"
                     /locus_tag="ACHKTW_004287"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO6465205.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDSVTLSLKVAALATLMALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGIVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     PLWLLNQLGRARDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            10669..12093
                     /gene="patD"
                     /locus_tag="ACHKTW_004288"
     CDS             10669..12093
                     /gene="patD"
                     /locus_tag="ACHKTW_004288"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019145 - aminobutyraldehyde dehydrogenase
                     (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HFO6465206.1"
                     /translation="MQHKLLINGELVSGEGEKQPVYNPATGDVLLEIAEASAEQVDAA
                     VRAADAAFAEWGQTTPKVRAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAARCLNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINVLFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHTASSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTAACRIYAQKGIYDTLVEKLGAAVATLKSGAPDDESTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTPFDNE
                     EQVVNWANDSQYGLASSVWTKDVGRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
BASE COUNT         2974 a         3002 c         3281 g         3029 t
ORIGIN      
        1 atcggtttct gtcagcgatt aaattcaaga ccacgtaaca aacgcctccg gcaattaatc
       61 cccagaacgc agaaccaatc ccgaccagcg tcaatccact tgccgttacc agaaatgcca
      121 ccaccgccgc gtcgcgctca cgctcattat gcagcgcctg atacaaactg ccgctaatgg
      181 tacttaacag cgccagacct gccagcatct ggatccaact tacgggcagg gcagccatca
      241 tcccggtaat agcactacca aacagacctg cgatcaaata aaaaatgcct gcaacggcag
      301 ctgccagcca acgttgatct ttatccggat gcgcttccgg gctttggcaa atagccgcgg
      361 tgattgccgc aataccgacg gaataaacgc cgaaagggga aaaaaccagt gccagcaatc
      421 cagtaaatac aattaatggc gaaacaggag ccgaataccc ggctgctttc attgctgcga
      481 tacccggtgc gttttgcgat gccatcgtca ccagaaaaag ggggagtgca acgctcaggc
      541 tgtgagcaaa cgaaaaatca ggggtaatat aagtggggag aacgggttta aagacaacat
      601 cagttgtgac aacgtcacct tgcgcgataa cgatcacgat cccaataatc atcgcggcaa
      661 ttaccgcata gcgcggcgca acggccttgg ttgccagcca taccagcaac atacttccac
      721 acaacgtaaa ttgaccgtcc agactggcaa acgcctgtaa accaaagcgt aataaaatcc
      781 cggcaagcat tgccgccgca agcgagtgcg gaataatgcg catcagacga gcaaagagtc
      841 ccgttatgcc gcagaggact attaacgcgt tggtgacaat aaaaacgccg atggcttcgt
      901 taagtgttag tccctgcaat ccggtgacca acaaagccgc gccaggcgtt gaccatgcgg
      961 tgagaacagg tacgcgatac cataatgtca gagtcagcgt actgacgccc attgccagcc
     1021 ccagcgccgt catccagcca gagatttgtg cagtggtggc tccggcgaca atcgctgctt
     1081 gccagattat tgccgctgaa ctggcgtagc caattaatac cgccagaaac cccgccagta
     1141 gcgtgggtgg aggaatagaa aacagacgca tagtcacctc gtgcgttata gcgtacaggc
     1201 accgtagcat ttgtccgtta taacgcacaa gtgataaact tccgttttgc cggaggagtc
     1261 gcatggaaaa tctcgctcgc tttttatcca ccacacttaa acaactacgc cagcagcgcg
     1321 gctggagtct ttcgcgactg gcagaagcga cgggcgtttc taaagcaatg ttagggcaga
     1381 ttgagcgtaa tgagtccagt ccgacggtcg cgacattatg gaaaattgcc accgggttga
     1441 acgtaccatt ttcaacattt atttctccgc cgcagtccgc tacgccttca gtttatgatc
     1501 cacaacagca ggcgatggtg atcacttcgc tgtttcctta tgatccacaa ctctgctttg
     1561 aacacttctc gattcagatg gcatctggtg caataagcga atcgacgccc catgagaagg
     1621 gggtaatcga acatgtcgtc gtcattgatg gacaacttga tctgtgcgtt gatggggaat
     1681 ggcagaccct taattgtggg gaaggcgttc gatttgctgc agacgtcacg catatctatc
     1741 gtaatggtgg ggagcaaacc gtacattttc attccctcat ccattacccg cgcagttaag
     1801 cgggaaaact atttcgcaac gtcgcgcttg tggctaaaat agccgccatt tttcagctac
     1861 tggataagaa tgtgaccgta tcttctcatc gacttgaact gttaagcccg gcacgcgatg
     1921 ccgccattgc ccgcgaagct attttgcacg gtgccgatgc tgtttatatc ggcggccctg
     1981 gttttggtgc ccgtcataat gccagtaata gcctgaaaga tattgccgag ctggtgccgt
     2041 ttgcccatcg ttatggtgca aaaattttcg tcacgcttaa caccattttg catgatgatg
     2101 agctggaacc cgcgcaacgg ctgattactg acctctacca gaccggtgtc gatgcgctga
     2161 ttgttcagga tatggggatt ctggaacttg atattccgcc gattgaactg cacgccagta
     2221 cgcagtgcga cattcgtaca gttgaaaaag cgaagttcct ctctgatgtt ggcttcacgc
     2281 agattgtgct ggcgcgagag ctgaatcttg atcagatccg cgcgattcac caggctacgg
     2341 acgcgaccat tgaattcttt attcatgggg cactgtgcgt ggcctattcg ggtcagtgct
     2401 acatttctca tgcgcaaaca gggcgtagcg ccaaccgtgg cgattgctcg caggcgtgcc
     2461 gtttgccata cacattgaaa gacgatcagg ggcgggtggt ttcctatgaa aaacatctgc
     2521 tgtcgatgaa agataacgat cagactgcca acctcggcgc gctgattgat gctggtgtac
     2581 gctccttcaa gattgaaggg cgttacaaag atatgagcta cgtgaagaat atcaccgccc
     2641 attatcgcca gatgcttgat gccattattg aagaacgtgg cgatctggcg cgcgcttcat
     2701 caggtcgtac tgaacatttc tttgttccat cgacggaaaa gactttccac cgtggtagca
     2761 cagattattt tgtgaatgcc cgtaaaggcg atattggcgc gttcgattcg ccgaaattta
     2821 tcggcctgcc ggtaggcgaa gtattgaaag tggcgaaaga tcatctcgat gttgccgtta
     2881 ccgagccact ggcaaatggc gatggcctga acgtgttgat taaacgtgaa gtcgtcggtt
     2941 ttcgtgccaa tacggtcgag aaaaccggag aaaatcagta ccgcgtctgg cccaatgaaa
     3001 tgccagcaga tttgcacaaa attcgtccac atcacccact aaaccgtaat cttgatcata
     3061 actggcagca ggcactgaca aaaacctcca gcgaacgtcg ggtggcggta gacattgaac
     3121 tgggcggctg gcaggaacaa ctgattctga ccctcaccag tgaagagggt gtcagcatca
     3181 cgcatacgct ggacgggcag ttcgacgaag ccaataacgc cgaaaaagca atgaacaatc
     3241 tgaaggatgg tctggcaaaa ctggggcaaa ccctctatta cgcccgcgat gtgcaaatta
     3301 atttgccggg ggcgctgttt gtaccaaaca gtctgttaaa ccagttccgc cgtgaagctg
     3361 ctgacatgct ggatgctgcg cgtcttgcca gttaccagcg cggcagccgt aaaccggttg
     3421 ctgatcctgc gccggtttat ccgcaaacgc atctgagttt cctcgcgaac gtatacaacc
     3481 agaaagcgcg tgaattttat catcgctatg gtgtgcagct gattgacgcg gcgtatgaag
     3541 cacatgaaga gaagggcgaa gtcccggtga tgatcaccaa gcattgtctg cgctttgcct
     3601 ttaatctgtg cccgaaacag gcgaaaggca atatcaaaag ctggaaggcg acgccaatgc
     3661 aactggttaa cggcgatgaa gtattaacgc taaagtttga ttgccgccca tgcgagatgc
     3721 acgtcattgg caaaatcaaa aatcacatac tgaaaatgcc gttaccggga agcgtagtgg
     3781 catccgtaag tccggatgag ctgctgaaaa cattgtttaa gcgaaaaggg taaaacgcca
     3841 gttttctggt tactcacaac tcattgaatc tgcatgatat tgcctgccgg gtaaggcgtc
     3901 tcgccgcatc cggcaacaaa tgactcaacg ttgtcccgct tctggtttgc gcgatttttg
     3961 ccagtaaaaa tgctcgcgca aaccttccgc cgactcttcc gccacagcac gcaattcatc
     4021 gctgtccgct tcatgacgca gctgatgatc cacattcttt acccacacaa attcatgtcc
     4081 tttgtgccct gccatgagtt gtcctgagaa cagcgcacac gttaataaga caaccgataa
     4141 cgccttcgta aacatcctgc cacctttttc ttaccttttg ccgctatgat gccgatcgtt
     4201 tcttgaggtt attattcagt tttgcaaatt agcgcaaaga aattctggaa tcttccttcc
     4261 tgattttgca tggcattctg ccgttgcggc gatttagtgc tattttcgag caaattacac
     4321 acggaggtaa acaataatgt ttactttgtt gatatactca gcggcaggga ggcgatgtga
     4381 aacaaagcga gttcagacgt tggctcgaat ctcagggcgt cgatgtagcg aatggcagca
     4441 accatttgaa actcaggttt catgggaggc gcagtgtcat gccgcgtcac ccctgcgatg
     4501 agattaaaga accattgcgt aaagcaatcc tgaaacaact cggtttgagt taatcgccaa
     4561 ttaaaaaggt taatgacatg cgagggacag tcgaaattat gcgttatccc gtcactctta
     4621 cacccgcgcc ggaaggcggt tatatggttt cttttgtgga aatccctgaa gcgttgacgc
     4681 agggcgaaac tgtcgctgaa gcgatggaag cggcaaaaga tgctttactg accgcatttg
     4741 atttttattt tgaagataac gagcttatcc ctttaccttc gccattaaat agtcatgatc
     4801 actttattga agtacctttg agcgtcgcct ctaaggtatt gctgttaaat gcttttttac
     4861 agtcagaaat cactcagcaa gagttagcca ggcgaattgg caaacctaaa caggagatta
     4921 ctcgcctatt taacttgcat catgcgacaa aaatcgacgc cgtccagctc gcggcaaagg
     4981 cgcttggcaa agagttatcg ctggtgatgg tttaattaca gttaacgaaa agttgtcatt
     5041 tttaacaact gatatagact gccgaatcat ctgcacataa ttacgattcg ataatgaaaa
     5101 aataccagca gcttgcagaa caattacgcg agcagattgc gtcgggtatc tggcaacccg
     5161 gcgatcgttt gccttcgttg cgtgaccagg tggcgctttc tggcatgagc tttatgacta
     5221 tcagccatgc ctatcagttg ctcgaaagtc tgggatatat tatcgcgcga ccgcagtcgg
     5281 gttattacgt tgcgccacag gcaataaaaa tgccgaaagc gccagtcatt ccagtcactc
     5341 gagatgaagc agtcgatatc aacacttata tttttgatat gttgcaggcc agtcgcgatc
     5401 cgtcggtcgt tccgtttgcc tcggcctttc ccgacccgcg acttttcccc ctccaacaac
     5461 taaaccgctc gctggcgcag gtaagcaaaa ccgccacagc gatgagcgtg attgaaaact
     5521 taccgccagg aaacgcagaa ctgcgtcagg ctattgctcg tcgctatgcc ttacagggca
     5581 tcaccatttc tcctgatgaa attgtcatta ctgccggggc gttagaggca ttaaacctca
     5641 gtttgcaagc ggtaactgaa ccgggcgatt gggtgatagt agagaatcct tgtttctacg
     5701 gtgcgttgca ggcgctggag cggctacggc tgaaggcgtt atcggtggcg acggatgtta
     5761 aagaagggat cgatcttcag gcgctggaac tggcgttgca ggattatccg gtgaaagcgt
     5821 gctggctgat gactaatagc cagaacccac tcggatttac cttaacgccg caaaaaaaag
     5881 cacaactggt ggcgttactc aatcagtaca acgtaacgct gattgaagat gacgtttaca
     5941 gcgaacttta ttttggacgg gaaaaaccgc tgcctgcgaa agcgtgggat cgccacgatg
     6001 gcgttttgca ttgctcttcg ttttcgaaat ttctggtgcc tggttttcgt attggttggg
     6061 tcgccgccgg aaaacatgca cgtaaaattc aacgcttgca gttgatgagt acgctttcca
     6121 ccagctcacc gatgcaactt gcgctggtgg attacctttc cacgcgccga tacgacgccc
     6181 atcttcgtcg cctgcgtcgc cagcttgcgg aacgtaaaca acgtgcctgg caggcactgc
     6241 tgcgttatct gcctgcggaa gttaaaattc atcatagtga cagcggttat tttctctggc
     6301 tggagctccc cgagccgtta gatgccggcg aattaagcct ggcggcactg acgcatcata
     6361 tcagtattgc gccgggtaaa atgttttcta ccggtgaaaa ctggtcacgt ttttttcgtt
     6421 ttaataccgc gtggcagtgg ggagaacgtg aagaacaggc ggtaaaacaa ttaggcaaac
     6481 ttattcaaga acggctgtaa tagcgtttaa tttaattcct cttagattgg gtaatatgaa
     6541 tttcgaatag cagtcatatt tcctaacccc ttgtctatac tccagaagat aaccttacag
     6601 acggcataat gcgcggtagc tcacaacctg attaaatttt ctcaggggcg aaggtgtgcc
     6661 tgcaagccgc cgtctatggt taaacaagga gatattttta cggcacggcg gcagaacaat
     6721 taattacgac aggagtaaga ccttatgagc aagacatttg cccgcagcag cctgtgtgcg
     6781 ctcaccatga caataatgac cgctcacgcc gccgaaccac ctaccaattt agataaaccg
     6841 gaagggcgac tggatattat cgcctggccg ggatacatcg aacgcggaca aactgataaa
     6901 caatacgact gggtaacgca atttgaaaaa gagacaggct gcgcggtgaa tgtgaaaacc
     6961 gccgcgactt ccgatgaaat ggtcagtctg atgaccaaag ggggttacga tctggttacg
     7021 gcatccggcg atgcctcgct gcgtttgatt atgggcaaac gcgtgcagcc gattaatacc
     7081 gccttgattc ccaactggaa aacgctcgat ccgcgcgtgg ttaaaggcga ctggtttaat
     7141 gttggcggca aagtttacgg cacaccttac caatgggggc cgaacctgct gatgtacaac
     7201 actaaaacct tcccgacgcc gccggatagc tggcaagtgg tttttgttga gcaaaatctg
     7261 ccggacggca agagcaataa aggccgcgtt caggcttatg atggccctat ctacattgcg
     7321 gacgctgcgt tgttcgttaa agccactcag ccgcagttgg gcatcagcga tccgtatcaa
     7381 ctaaccgaag aacagtacca ggcggtgctg aaagtgctgc gcgctcaaca cagtttgatc
     7441 catcgctact ggcatgacac taccgtgcaa atgagcgatt tcaaaaacga gggtgtggtt
     7501 gcttccagtg cctggcccta tcaggccaac gccctgaaag ccgaaggcca gcctgttgct
     7561 accgttttcc cgaaggaggg tgttaccggt tgggctgata ccaccatgct gcatagcgaa
     7621 gcgaaacatc cggtttgcgc ctacaaatgg atgaactggt cattaacgcc aaaagtgcag
     7681 ggcgatgtgg cggcctggtt tggctcgtta ccggtagtgc cggaagggtg taaagccagt
     7741 ccgttattag gcgaaaaagg ttgtgaaacc aacggtttta actatttcga caaaatcgcc
     7801 ttctggaaaa cgcctatagc agaagggggc aagtttgttc cctacagtcg ctggacgcag
     7861 gattacattg ccattatggg cggtcgctaa cttcgctggg gtgctttatg acgtacgcag
     7921 tggagtttga caacgtctcg cggttgtacg gtgacgtgcg cgcagtagat ggcgtcagta
     7981 ttgcgataaa agatggtgag ttcttctcta tgctggggcc gtccggctcc ggcaaaacca
     8041 cctgcctgcg cctgattgct ggcttcgaac agctttccgg cggggctatc tctatctttg
     8101 gtaaacccgc cagcaatctg ccaccgtggg agcgggacgt gaatactgtc tttcaggact
     8161 acgcgctatt tccgcatatg tcgattcttg acaatgtcgc ctatgggctg atggtcaaag
     8221 gcgtgaataa aaagcagcgg cacgcaatgg cgcaagaggc gctggagaaa gtggcgttgg
     8281 ggtttgtaca tcaacgtaaa ccgtcacaac tttctggtgg tcagcgccag cgggttgcta
     8341 tcgccagagc attggtgaat gaaccgcgcg tattgctgtt ggatgaaccg ctcggcgcac
     8401 tggatctcaa attgcgtgag cagatgcagc tggaactgaa aaaactgcaa cagtctctcg
     8461 gtatcacttt tatcttcgtt acccacgatc agggcgaagc gttatcgatg tccgatcgtg
     8521 tggcggtttt caataatgga cgcattgagc aggtcgattc cccgcgcgat ctctatatgc
     8581 gcccgcgcac gccgtttgtt gccggattcg tcgggacatc caacgttttt gatggactga
     8641 tggcagagaa actttgtggc atgacgggaa gcttcgccct gcgaccggaa catatccgcc
     8701 tcaacacccc tggtgaactg caggccaatg gcacgatcca ggcggtgcaa tatcagggcg
     8761 cggcaactcg ttttgaactg aaattgaacg gcggtgaaaa actgcttgtg agtcaggcca
     8821 atatgacagg cgaagaactg cctgccacgc tcacgcccgg acaacaggtg atggtttcct
     8881 ggtcgcgtga tgtgatggtg ccgctggttg aggagaggtg aatggcgatg aatgtattgc
     8941 aatcaccttc acgtccaggt ctgggtaagg tgtccggttt cttctggcgt aatccggggc
     9001 tggggctgtt tttactgctg cttggcccgc taatgtggtt tggcattgtc tatttcggct
     9061 cgctgctgac actgttatgg cagggatttt atacttttga cgatttcacc atgtcggtaa
     9121 cgccggaact gacgctggcg aatatccgtg cgctgtttaa tccggcgaat tacgacatca
     9181 ttctccgcac gctgactatg gctgtggcag tcactatcgc cagcgccatt ctggcttttc
     9241 caatggcgtg gtatatggcg cgctatacca gcgggaaaat gaaagctttt ttttatattg
     9301 cggtaatgtt gccgatgtgg gcgagctaca ttgttaaagc ctatgcctgg acgttattgc
     9361 tggcaaaaga tggcgtggct cagtggtttt tacaacatct tgggctggaa ccactgctga
     9421 ctgcgttcct tacattacct gcggtgggcg gaaatacgct gtcaacttcc gggctggggc
     9481 gctttctggt gtttctctat atctggttgc cgttcatgat cctgcccgtt caggcggcgc
     9541 ttgagcgttt gccgccgtca ttgttgcagg cgtcggctga tctcggcgca cgtccacgac
     9601 aaacctttcg ctatgtggtg ctgccgctgg caatcccggg tattgccgct ggctctatct
     9661 ttaccttctc actcacactg ggcgatttta tcgtcccgca gctggttggt cctccaggat
     9721 attttatcgg caatatggtt tattcccagc agggggcgat tggcaatatg ccgatggcgg
     9781 cggcattcac cctggtgccg attgttctca tcgcactgta cctggcgttc gtgaaacgtc
     9841 tgggagcgtt cgatgcactc tgaacgcgca ccgtttttcc tcaaactggc ggcctggggc
     9901 ggcgtggttt tcctacattt tcccatcctg attatcgccg cttatgcgtt taacactgaa
     9961 gatgcggcgt ttagttttcc accgcagggc ctgacgctgc gctggtttag cgtggcagca
    10021 cagcgtagtg atattcttga ttccgtgaca ctgtcactta aagtggcggc gctggcgaca
    10081 ttaatggcgc tggtgttagg gacgctggca gctgccgcgc tgtggcgacg agactttttc
    10141 ggcaaaaacg ccatttcgct gttactgctg ctgcccattg cgctgccggg cattgtcact
    10201 ggtctggcgc tattaaccgc ttttaaaacc atcaatctgg agccgggatt tttcaccatc
    10261 gtggtcggtc atgcgacttt ttgcgtagtg gtggtgttta acaatgtcat cgcccgtttt
    10321 cgccgcacct cgtggagtct ggttgaggcg tcaatggatc ttggggccaa tggctggcaa
    10381 accttccgct acgtagtgtt gccgaatctc agttcggcgc tactggcagg aggaatgctg
    10441 gcgtttgcct tgtcgttcga tgaaatcatc gttacgactt ttacggcagg tcatgaacga
    10501 acgttaccgt tgtggttgct caatcagctt gggcgagcgc gtgatgtacc ggtaactaac
    10561 gtggtggcac tgctggttat gttggtaaca accttgccga tcctgggggc ctggtggcta
    10621 acccgcgaag gcgacaatgg tcaataacca ctgatacagg aatatgctat gcaacataag
    10681 ttactgatta acggagaact ggttagcggc gaaggggaaa aacagcctgt ctataatccg
    10741 gcaacggggg acgttttact ggagattgcc gaggcatccg cagagcaggt cgatgctgct
    10801 gtgcgcgcgg cagatgcagc atttgccgaa tgggggcaaa ccacgccgaa agtgcgtgcg
    10861 gaatgtctgc tgaaactggc tgatgttatc gaagaaaatg gtcaggtttt tgccgaactg
    10921 gagtcccgta attgtggcaa accgctgcat agtgcgttca atgatgaaat cccggcgatt
    10981 gtcgatgttt ttcgcttttt cgcgggtgcg gcgcgctgtc tgaatggtct ggcggcaggt
    11041 gaatatcttg aaggtcatac ttcgatgatc cgtcgcgatc cgttgggggt cgtggcttct
    11101 atcgcaccgt ggaattatcc gctgatgatg gcagcgtgga aactggctcc ggcgctggcg
    11161 gcagggaact gcgtagtgct taaaccatca gaaattaccc cgctgaccgc gttgaagttg
    11221 gcagagctgg cgaaagatat cttcccagca ggcgtgatta acgtactgtt tggcagaggc
    11281 aaaacggtgg gtgatccgct gaccggtcat cccaaagtgc ggatggtgtc gctgacgggc
    11341 tctatcgcca ccggcgagca catcatcagc cataccgcgt cgtccattaa gcgtactcat
    11401 atggaacttg gtggcaaagc gccagtgatt gtttttgatg atgcggatat tgaagcagtg
    11461 gtcgaaggtg tacgtacatt tggctattac aatgctggac aggattgtac tgcggcttgt
    11521 cggatctacg cgcaaaaagg catttacgat acgctggtgg aaaaactggg tgctgcggtg
    11581 gcaacgttaa aatctggtgc gccggatgac gagtctacgg agcttggacc tttaagctcg
    11641 ctggcgcatc tcgaacgcgt cagcaaggca gtagaagagg cgaaagcgac agggcacatc
    11701 aaagtgatca ctggcggtga aaagcgcaag ggtaatggct attactatgc gccgacgctg
    11761 ctggctggcg cattacagga cgatgccatc gtgcaaaaag aggtatttgg tccagtagtg
    11821 agtgttacgc ccttcgacaa cgaagaacag gtggtgaact gggcgaatga cagccagtac
    11881 ggacttgcat cttcggtatg gacgaaagat gtgggcaggg cgcatcgcgt cagcgcacgg
    11941 ctgcaatatg gttgtacctg ggtcaatacc catttcatgc tggtaagtga aatgccgcac
    12001 ggtgggcaga aactttctgg ttacggcaag gatatgtcac tttatgggct ggaggattac
    12061 accgtcgtcc gccacgtcat ggttaaacat taagttagat gttagtggct gttataggac
    12121 ggccactatc gttttaggtt ctgagtttaa tggcggttaa gtattagaat gattacatta
    12181 ataactagcg taaaaattag gctaaatgtt tgatggcgat ttttcatcct gtcacagttg
    12241 attagcataa ggctttagag tatcgttatt aaactaataa tttata
//
LOCUS       DAZFQD010000150        12261 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.150, whole genome shotgun sequence.
ACCESSION   DAZFQD010000150 DAZFQD010000000
VERSION     DAZFQD010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12261
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.150"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            813..1055
                     /locus_tag="ACHKTW_004289"
     CDS             813..1055
                     /locus_tag="ACHKTW_004289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase"
                     /protein_id="HFO6465207.1"
                     /translation="MKTAALDLARERQAHEAGARTRATAHERTPQQERQKAAREAERG
                     REAWTLGQGMKKPVAGCYGRLTRWKGGGDVVYMALL"
     gene            complement(1087..1737)
                     /gene="tetR(A)"
                     /locus_tag="ACHKTW_004290"
     CDS             complement(1087..1737)
                     /gene="tetR(A)"
                     /locus_tag="ACHKTW_004290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(A)"
                     /protein_id="HFO6465208.1"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWH
                     FRNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAG
                     TRPGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGG
                     TVEQAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     gene            1843..3042
                     /gene="tet(A)"
                     /locus_tag="ACHKTW_004291"
     CDS             1843..3042
                     /gene="tet(A)"
                     /locus_tag="ACHKTW_004291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008493 - tetracycline transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015904 - tetracycline transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(A)"
                     /protein_id="HFO6465209.1"
                     /translation="MKPNRPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAH
                     YGILLALYALMQFACAPVLGALSDRFGRRPVLLVSLAGAAVDYAIMATAPFLWVLYIG
                     RIVAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPH
                     APFFAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAV
                     FFIMQLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGE
                     RRALMLGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQG
                     QLQGSLAALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSG
                     AGQRADR"
     gene            complement(3074..3958)
                     /locus_tag="ACHKTW_004292"
     CDS             complement(3074..3958)
                     /locus_tag="ACHKTW_004292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HFO6465210.1"
                     /translation="MSLRTPDLLFTAIAPAIWGSTYIVTTQYLPNFSPMTVAMLRALP
                     AGLLLVMIVRQIPTGIWWMRIFILGALNISLFWSLLFISVYRLPGGVAATVGAVQPLM
                     VVFISAALLGSPIRLMAVLGAICGTAGVALLVLTPNAALDPVGVAAGLAGAVSMAFGT
                     VLTRKWQPPVPLLTFTAWQLAAGGLLLVPVALVFDPPIPMPTGTNVLGLAWLGLIGAG
                     LTYFLWFRGISRLEPTVVSLLGFLSPGTAVLLGWLFLDQTLSALQIIGVLLVIGSIWL
                     GQRSNRTPRARIACRKSP"
     gene            complement(4096..4488)
                     /locus_tag="ACHKTW_004293"
     CDS             complement(4096..4488)
                     /locus_tag="ACHKTW_004293"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFO6465211.1"
                     /translation="MLFPNFSLGEEYEHAPPATNRQISPYLPSGRFRTGLPVEGLAIE
                     RGDLFYACPRASVFYGTALDADLRTRGVSTLVMAGISTTGVVLSSVAWASDADYDVRL
                     VQDCCYDPDRDAHEALLRSGFGGRVQVV"
     gene            4492..6243
                     /locus_tag="ACHKTW_004294"
                     /pseudo
     CDS             4492..6243
                     /locus_tag="ACHKTW_004294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138014.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element TnAs1 family transposase"
     gene            complement(6240..6749)
                     /locus_tag="ACHKTW_004295"
     CDS             complement(6240..6749)
                     /locus_tag="ACHKTW_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001376611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6465212.1"
                     /translation="MSRIPFLRSYSASISAAFLSAFCASFCRFSACSINDLASRGDIR
                     RESASANSIRLSVCSRRASYPAISVRNPYSLSSACRSLNSCRVISAASAFPARSVFSV
                     FSSFSLAAIAAERRFPASVRLFSFSFCCALSVFLYAVISLSLFSISLRVGESSTHSGS
                     PQNSEKIVR"
     gene            6709..7983
                     /locus_tag="ACHKTW_004296"
     CDS             6709..7983
                     /locus_tag="ACHKTW_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001477385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0031640 - killing of cells of another
                     organism [Evidence IEA];
                     GO_process: GO:0050829 - defense response to Gram-negative
                     bacterium [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colicin Ia central receptor-binding
                     domain-containing protein"
                     /protein_id="HFO6465213.1"
                     /translation="MLAEYERRKGILDTRLSELEKNGGAALAVLDAQQARLLGQQTRN
                     DRAISEARNKLSSVTESLNTARNALTRAEQQLTQQKNTPDGKTIVSPEKFPGRSSTNH
                     SIVVSGDPRFAGTIKITTSAVIDNRANLNYLLTHSGLDYKRNILNDRNPVVTEDVEGD
                     KKIYNAEVAEWDKLRQRLLDARNKITSAESAINSARNNVSARTNEQKHANDALNALLK
                     EKENIRSQLADINQKIAEEKRKRDEINMVKDAIKLTSDFYRTIYDEFGKQASELAKEL
                     ASVSQGKQIKSVDDALNAFDKFRNNLNKKYSIQDRMAISKALEAINQVHMAENFKLFS
                     KAFGFTGKVIDRYDVAVELQKAVKTDNWRPFFVKLESLAAGRAASAVTAWTFSVMLGT
                     PVGILGFAIIMAAVSALVNDKFIEQVNKLIGI"
     gene            complement(8001..8348)
                     /locus_tag="ACHKTW_004297"
     CDS             complement(8001..8348)
                     /locus_tag="ACHKTW_004297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015643 - toxic substance binding
                     [Evidence IEA];
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E1 family microcin immunity protein"
                     /protein_id="HFO6465214.1"
                     /translation="MKLDISVKYLLKSLIPSLIILTVFYLGWKDNQENARMFYAFIGC
                     IINAITFPFSMRIIQKMVIKFTGKELWQKDFFTNPVGGSLTAIFELFCFVISVPVVAI
                     YLIFIFCKALSGK"
     gene            8467..8814
                     /locus_tag="ACHKTW_004298"
     CDS             8467..8814
                     /locus_tag="ACHKTW_004298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6404 family protein"
                     /protein_id="HFO6465215.1"
                     /translation="MTFEQKKARAIALMDSKKMWRSNYAPPLLRILWRLGIRLPPLPF
                     MPFWQVTVLMGGLWGISWGCAMWFIYWGPSGMVAGEAIIISITGGFLFGLCMASFHWW
                     RRKVNRLPPWDDV"
     gene            complement(8834..9019)
                     /locus_tag="ACHKTW_004299"
                     /pseudo
     CDS             complement(8834..9019)
                     /locus_tag="ACHKTW_004299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545742.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(9410..9712)
                     /locus_tag="ACHKTW_004300"
     CDS             complement(9410..9712)
                     /locus_tag="ACHKTW_004300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6465216.1"
                     /translation="MRNRANTSPRDEAAFINGGTAGLDNTKKSSDKSSVVSKAKPVSI
                     SFFEENLKDIDALITAEMVSGKGRVNRSDVVRAAVEALKTLSKTEVSRLIAEVKQR"
     gene            complement(9709..10335)
                     /locus_tag="ACHKTW_004301"
     CDS             complement(9709..10335)
                     /locus_tag="ACHKTW_004301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010260829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family plasmid-partitioning AAA ATPase"
                     /protein_id="HFO6465217.1"
                     /translation="MIIVLGSQKGGVGKSTLAVSIAAYLMSLGNRVIIVDADDQKSVL
                     TWYNNRPEDLPHIPVTGATGNIKAMLKEHEKSYDFVIADCAGRDSAEMRSGLMAADVF
                     ISPLRPSQMDLDVVPHTCSVFTAAKDFNEDVRGYLVLNMTPTNMFVNEANEAAEVLKD
                     YPEMHLANSRVCDRKAHRDAWAESMTIFETQNDKAQQEIEALVKEVIL"
     gene            complement(10555..11826)
                     /locus_tag="ACHKTW_004302"
     CDS             complement(10555..11826)
                     /locus_tag="ACHKTW_004302"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001744069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0019985 - translesion synthesis [Evidence
                     IEA];
                     GO_process: GO:0042276 - error-prone translesion synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFO6465218.1"
                     /translation="MFALADINSFYASCEKVFRPDLRNEPVIVLSNNDGCVIARSPEA
                     KALGIRMGQPRFQVRQMRLEKKIHVFSSNYALYHSMSQRVMAVLESLSPAVEPYSIDE
                     MFIDLRGINHCISPEFFGHQLREQVKSWTGLTMGVGIAPTKTLAKSAQWATKQWPQFS
                     GVVALTAENRNRILKLLGLQPVGEVWGVGRRLTEKLNALGINTALQLAQANTAFIRKN
                     FSVILERTVRELNGESCISLEEAPPAKQQIVCSRSFGERITDKDAMHQAVVQYAERAA
                     EKLRGERQYCRQVTTFVRTSPFAVKEPCYSNAAVEKLSLPTQDSRDIIAAACRALNHV
                     WREGYRYMKAGVMLADFTPSGIAQPGLFDEIQPRKNSEKLMKTLDELNQSGKGKVWFA
                     GRGTAPEWQMKREMLSPAYTTRWTDLPVAQL"
     gene            complement(11826..>12261)
                     /gene="umuD"
                     /locus_tag="ACHKTW_004303"
     CDS             complement(11826..>12261)
                     /gene="umuD"
                     /locus_tag="ACHKTW_004303"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HFO6465219.1"
                     /translation="STVYHRPADPSGDDSYVRPLFADRCQAGFPSPATDYAEQELDLN
                     SYCISRPAATFFLRASGESMNQAGVQNGDLLVVDRGEKPQHGDIVIAEIDGEFTVKRL
                     LLRPRPALEPVSDSPEFRTLYPENICIFGVVTHVIHRTRELR"
BASE COUNT         2843 a         3408 c         3300 g         2710 t
ORIGIN      
        1 gcacccccct tcggcgtgcg cggtcacgcg cacagggcgc agccctggtt aaaaacaagg
       61 tttataaata ttggtttaaa agcaggttaa aagacaggtt agcggtggcc gaaaaacggg
      121 cggaaaccct tgcaaatgct ggattttctg cctgtggaca gcccctcaaa tgtcaatagg
      181 tgcgcccctc atctgtcagc actctgcccc tcaagtgtca aggatcgcgc ccctcatctg
      241 tcagtagtcg cgcccctcaa gtgtcaatac cgcagggcac ttatccccag gcttgtccac
      301 atcatctgtg ggaaactcgc gtaaaatcag gcgttttcgc cgatttgcga ggctggccag
      361 ctccacgtcg ccggccgaaa tcgagcctgc ccctcatctg tcaacgccgc gccgggtgag
      421 tcggcccctc aagtgtcaac gtccgcccct catctgtcag tgagggccaa gttttccgcg
      481 aggtatccac aacgccggcg gccgcggtgt ctcgcacacg gcttcgacgg cgtttctggc
      541 gcgtttgcag ggccatagac ggccgccagc ccagcggcga gggcaaccag cccggtgagc
      601 gtcggaaagg cgctggaagc cccgtagcga cgcggagagg ggcgagacaa gccaagggcg
      661 caggctcgat gcgcagcacg acatagccgg ttctcgcaag gacgagaatt tccctgcggt
      721 gcccctcaag tgtcaatgaa agtttccaac gcgagccatt cgcgagagcc ttgagtccac
      781 gctagatcta tctcatctgc gcaaggcaga acgtgaagac ggccgccctg gacctcgccc
      841 gcgagcgcca ggcgcacgag gccggcgcgc ggacccgcgc cacggcccac gagcggacgc
      901 cgcagcagga gcgccagaag gccgccagag aggccgagcg cggccgtgag gcttggacgc
      961 tagggcaggg catgaaaaag cccgtagcgg gctgctacgg gcgtctgacg cggtggaaag
     1021 ggggagggga tgttgtctac atggctctgc tgtagtgagt gggttgcgct ccggcagcgg
     1081 tcctgatcaa tcgtcaccct ttctcggtcc ttcaacgttc ctgacaacga gcctcctttt
     1141 cgccaatcca tcgacaatca ccgcgagtcc ctgctcgaac gctgcgtccg gaccggcttc
     1201 gtcgaaggcg tctatcgcgg cccgcaacag cggcgagagc ggagcctgtt caacggtgcc
     1261 gccgcgctcg ccggcatcgc tgtcgccggc ctgctcctca agcacggccc caacagtgaa
     1321 gtagctgatt gtcatcagcg cattgacggc gtccccggcc gaaaaacccg cctcgcagag
     1381 gaagcgaagc tgcgcgtcgg ccgtttccat ctgcggtgcg cccggtcgcg tgccggcatg
     1441 gatgcgcgcg ccatcgcggt aggcgagcag cgcctgcctg aagctgcggg cattcccgat
     1501 cagaaatgag cgccagtcgt cgtcggctct cggcaccgaa tgcgtatgat tctccgccag
     1561 catggcttcg gccagtgcgt cgagcagcgc ccgcttgttc ctgaagtgcc agtaaagcgc
     1621 cggctgctga acccccaacc gttccgccag tttgcgtgtc gtcagaccgt ctacgccgac
     1681 ctcgttcaac aggtccaggg cggcacggat cactgtattc ggctgcaact ttgtcatgct
     1741 tgacacttta tcactgataa acataatatg tccaccaact tatcagtgat aaagaatccg
     1801 cgcgttcaat cggaccagcg gaggctggtc cggaggccag acgtgaaacc caacagaccc
     1861 ctgatcgtaa ttctgagcac tgtcgcgctc gacgctgtcg gcatcggcct gattatgccg
     1921 gtgctgccgg gcctcctgcg cgatctggtt cactcgaacg acgtcaccgc ccactatggc
     1981 attctgctgg cgctgtatgc gttgatgcaa tttgcctgcg cacctgtgct gggcgcgctg
     2041 tcggatcgtt tcgggcggcg gccggtcttg ctcgtctcgc tggccggcgc tgctgtcgac
     2101 tacgccatca tggcgacggc gcctttcctt tgggttctct atatcgggcg gatcgtggcc
     2161 ggcatcaccg gggcgactgg ggcggtagcc ggcgcttata ttgccgatat cactgatggc
     2221 gatgagcgcg cgcggcactt cggcttcatg agcgcctgtt tcgggttcgg gatggtcgcg
     2281 ggacctgtgc tcggtgggct gatgggcggt ttctcccccc acgctccgtt cttcgccgcg
     2341 gcagccttga acggcctcaa tttcctgacg ggctgtttcc ttttgccgga gtcgcacaaa
     2401 ggcgaacgcc ggccgttacg ccgggaggct ctcaacccgc tcgcttcgtt ccggtgggcc
     2461 cggggcatga ccgtcgtcgc cgccctgatg gcggtcttct tcatcatgca acttgtcgga
     2521 caggtgccgg ccgcgctttg ggtcattttc ggcgaggatc gctttcactg ggacgcgacc
     2581 acgatcggca tttcgcttgc cgcatttggc attctgcatt cactcgccca ggcaatgatc
     2641 accggccctg tagccgcccg gctcggcgaa aggcgggcac tcatgctcgg aatgattgcc
     2701 gacggcacag gctacatcct gcttgccttc gcgacacggg gatggatggc gttcccgatc
     2761 atggtcctgc ttgcttcggg tggcatcgga atgccggcgc tgcaagcaat gttgtccagg
     2821 caggtggatg aggaacgtca ggggcagctg caaggctcac tggcggcgct caccagcctg
     2881 acctcgatcg tcggacccct cctcttcacg gcgatctatg cggcttctat aacaacgtgg
     2941 aacgggtggg catggattgc aggcgctgcc ctctacttgc tctgcctgcc ggcgctgcgt
     3001 cgcgggcttt ggagcggcgc agggcaacga gccgatcgct gatcgtggaa acgataggcc
     3061 tatgccatgc gggtcaaggc gacttccggc aagctatacg cgccctagga gtgcggttgg
     3121 aacgttggcc cagccagata ctcccgatca cgagcaggac gccgatgatt tgaagcgcac
     3181 tcagcgtctg atccaagaac aaccatccta gcaacacggc ggtccccggg ctgagaaagc
     3241 ccagtaagga aacaactgta ggttcgagtc gcgagatccc ccggaaccaa aggaagtagg
     3301 ttaaacccgc tccgatcagg ccgagccacg ccaggccgag aacattggtt cctgtaggca
     3361 tcgggattgg cggatcaaac actaaagcta ctggaacgag cagaagtcct ccggccgcca
     3421 gttgccaggc ggtaaaggtg agcagaggca cgggaggttg ccacttgcgg gtcagcacgg
     3481 ttccgaacgc catggaaacc gcccccgcca ggcccgctgc gacgccgaca ggatctagcg
     3541 ctgcgtttgg tgtcaacacc aacagcgcca cgcccgcagt tccgcaaata gcccccagga
     3601 ccgccatcaa tcgtatcggg ctacctagca gagcggcaga gatgaacacg accatcagcg
     3661 gctgcacagc gcctaccgtc gccgcgaccc cgcccggcag gcggtagacc gaaataaaca
     3721 acaagctcca gaatagcgaa atattaagtg cgccgaggat gaagatgcgc atccaccaga
     3781 ttcccgttgg aatctgtcgg acgatcatca cgagcaataa acccgccggc aacgcccgca
     3841 gcatcgcgac cgtcatcggt gagaagttcg gcaggtattg ggtggtgaca atgtaggtgc
     3901 tgccccaaat ggcaggtgct atcgctgtga acaataaatc gggcgtgcgt aatgacatgc
     3961 gattacaaga cctccgctac ggcgatgggt ttttgagagt tgcaggcgcc gcgatgaccg
     4021 caccctgact tggactggcc ctgcctcgca ccaatccacc tgcggctatg gccgcgatca
     4081 tgcgaggccc gaaaattaca cgacctgtac acgtccgccg aacccggaac gcaacagagc
     4141 ttcgtgggca tcccgatccg ggtcgtagca gcagtcctgg accaaacgca cgtcgtagtc
     4201 cgcatcacta gcccaggcga cgcttgaaag aacaacgccg gtggtgctta tcccggccat
     4261 gacaagcgtg cttacgccgc gcgtccgaag gtcggcgtcg agcgccgtgc cataaaagac
     4321 gctggctcgc ggacatgcat agaaaaggtc gccccgttcg atcgcaagcc cttcgacggg
     4381 cagaccggtg cgaaatcgtc cgctcgggag atacggtgag atttggcgat tcgtcgccgg
     4441 cggcgcatgt tcatactctt cgccgagcga aaagttgggg aacagcacgg gccggccgcg
     4501 caaggcgtgc tggcagccgt gcagaccctg cgtgagatga acgccgacaa cctgcgcaag
     4561 gtgccggccg atgcacccac ggccttcatc aagccgcgct ggaagccgct ggtgatcacc
     4621 ccggaaggcc tcgaccggaa attctacgaa atctgcgccc tgtccgagct gaagaacgcc
     4681 ctgcgctccg gcgacatctg ggtcaagggc tcgcggcagt tccgcgactt cgacgactac
     4741 ctgctgccgg ccgagaagtt cgccgcactc aagcgcgagc aggccctgcc cctggcgatc
     4801 aacccgaaca gcgaccagta cctggaagag cgtttgcagc tgctggacga gcagttggcc
     4861 accgtcaccc gcctggccaa ggacaacgag ctgcccgatg ccatcctcac cgagtcaggg
     4921 ctgaaaatca ccccgctgga tgcggcggtg ccggatcggg cgcaggcgct gatcgaccaa
     4981 accagccagt tactgccgcg catcaagatc accgaactgc tgatggacgt ggacgactgg
     5041 acgggcttca gccgccactt cacccacttg aaggacgggg ccgaggccaa agacaggacg
     5101 ttgctgctgt ccgcaatcct cggtgatgcg atcaacctcg ggctgaccaa gatggccgag
     5161 tcgagccccg gcctgaccta cgccaagctg tcctggctgc aagcctggca catccgcgac
     5221 gaaacctatt cggcggcctt ggccgagctg gtcaaccacc agtatcgcca cgcctttgcc
     5281 gcccactggg gcgacggcac gacctcatcc tccgatggcc agcgcttccg cgcgggtggc
     5341 cggggcgaga gcaccgggca cgtcaacccg aagtacggta gcgagccggg acggctgttc
     5401 tatacccata tctccgacca gtacgcgccg ttcagcaccc gcgtggtgaa tgtcggcgtc
     5461 cgcgattcca cctatgtgct cgacggcctg ctgtaccacg agtccgacct gcggatcgag
     5521 gagcactaca ccgacacggc cggcttcacc gatcacgtct ttgccctgat gcacctgcta
     5581 ggcttccgct tcgcgccgcg catccgcgac ctcggcgaaa ccaagctgta cgtgccgcag
     5641 ggcgtgcaag cctacccgac gttgcgcccg ctgatcggcg gcaccctgaa catcaagcac
     5701 gtgcgtgccc actgggacga catcctgcgc ctggccagct cgatcaagca gggcaccgtc
     5761 accgcctcgc tgatgctgcg caagctcggc agctacccgc gccagaacgg actggccgtg
     5821 gccctgcgcg agctgggccg gatcgagcgc acgctgttca tcctggactg gctgcaaagt
     5881 gttgaactgc gccgccgcgt gcatgccggc ctgaacaaag gtgaggcgcg caactcgctg
     5941 gccagggcgg tgttcttcaa ccgccttggg gaaatcaggg atcggagctt cgagcagcag
     6001 cgctaccggg ccagcggcct caacctggtg acggcggcta tcgtgctgtg gaacacggtg
     6061 tacctggaac gcgccaccca ggggttggtc gaggccggca agccggtgga cggcgagctg
     6121 ctgcaattcc tgtcgccgct gggctgggag cacatcaacc taaccggcga ttacgtctgg
     6181 cggcagagcc gcagactgga agacgggaag tttcggccct tacggatgcc cggaaaacct
     6241 tagcgtacga ttttttccga attctgcggg ctccccgagt gggtcgatga ttccccaacc
     6301 cgaagtgata tcgaaaaaag ggacaaggaa atcacagcgt acaaaaacac gctcagcgcg
     6361 cagcagaaag aaaatgagaa taagcgcact gaagccggaa aacgcctctc tgcggcgatt
     6421 gctgcaaggg aaaaagatga aaacacactg aaaacactcc gtgccggaaa cgcagatgcc
     6481 gctgatatta cacgacagga gttcagactc ctgcaggcag agctgagaga atacggattc
     6541 cgtactgaaa tcgccggata tgacgccctc cggctgcata cagagagccg gatgctgttt
     6601 gctgatgctg attctcttcg tatatctccc cgggaggcca ggtcgttaat cgaacaggct
     6661 gaaaaacggc agaaggatgc gcagaacgca gacaagaagg ccgctgatat gcttgctgaa
     6721 tacgagcgca gaaaaggtat tctggacacg cggttgtcag agctggaaaa aaatggcggg
     6781 gcagcccttg ccgttcttga tgcacaacag gcccgtctgc tcgggcagca gacacggaat
     6841 gacagggcca tttcagaggc ccggaataag ctcagttcgg tgacggaatc gcttaacacg
     6901 gcccgtaatg cattaaccag agctgaacaa cagctgacgc aacagaaaaa cacacctgac
     6961 ggcaaaacga tagtttcccc tgaaaaattc ccggggcgtt catcaacaaa tcattctatt
     7021 gttgtgagcg gtgatccgag atttgccggt acgataaaaa tcacaaccag cgcagtcatc
     7081 gataaccgtg caaacctgaa ttatcttctg acccattccg gtctggacta taaacgcaat
     7141 attctgaatg accggaatcc ggtggttaca gaggatgtgg aaggtgacaa gaaaatttat
     7201 aatgctgaag ttgctgaatg ggataagtta cggcaacgat tgcttgatgc cagaaataaa
     7261 atcacctctg ctgaatctgc gataaattcg gcgagaaata acgtcagtgc cagaacaaat
     7321 gaacaaaagc atgcaaatga cgctcttaat gccctgttga aggaaaaaga gaatatccgt
     7381 agccagcttg ctgacatcaa tcagaaaata gcggaagaga aaagaaaaag ggatgaaata
     7441 aatatggtaa aggatgccat aaaactcacc tctgatttct acagaacgat atatgatgag
     7501 ttcggtaaac aagcatccga acttgctaag gagctggctt ctgtatctca agggaaacag
     7561 attaagagtg tggatgatgc actgaacgct tttgataaat tccgtaataa tctgaacaag
     7621 aaatatagca tacaagatcg catggccatt tctaaagccc tggaagctat taatcaggtc
     7681 catatggcgg agaattttaa gctgttcagt aaggcatttg gttttaccgg aaaagttatt
     7741 gatcgttatg atgttgctgt ggagttacaa aaggctgtaa aaacggacaa ctggcgtcca
     7801 ttttttgtaa aacttgaatc actggcagca ggaagagctg cttcagcagt tacagcatgg
     7861 acgttttccg tcatgctggg aacccctgta ggtattctgg gttttgcaat tattatggcg
     7921 gctgtgagtg cgcttgttaa tgataagttt attgagcagg tcaataaact tattggtatc
     7981 tgacacataa tgatgccctt tcattttcct gaaagggctt tgcagaatat aaaaattaag
     8041 taaatggcaa ccacaggaac tgatataacg aaacagaata attcaaatat tgcagttaag
     8101 cttccgccaa ctggatttgt aaagaagtct ttttgccaga gttctttccc tgtaaacttt
     8161 attaccattt tctgtattat cctcattgaa aaaggaaaag taatggcatt gataatgcat
     8221 ccgatgaacg cataaaacat tcttgcattt tcctggttat ctttccatcc cagataaaaa
     8281 actgtaagaa taatgaggct tggtatcagg cttttcagta aatactttac agatatatcc
     8341 agtttcatct ggtttctcct ttattgttat cgtgtaccgg tgcgtgataa cataatcaca
     8401 gcatgataat aataatcaat aacaataagc tgtgtcacgt ttacatcatg gaacggaggt
     8461 gctcctgtga cctttgagca gaaaaaagcc cgggccattg ccctgatgga cagcaaaaag
     8521 atgtggcgga gcaattatgc ccctcctctt ctgcgtattc tatggcggct gggtatccgg
     8581 cttccgccat taccctttat gccgttctgg caggtcacgg tgctgatggg gggcttatgg
     8641 ggcatttcct ggggatgtgc aatgtggttt atctactggg ggccgtcagg aatggtggca
     8701 ggtgaggcca ttattataag tatcacgggt ggttttttgt tcggactgtg catggcctct
     8761 ttccactggt ggcgcaggaa agttaaccgg ctgccaccat gggatgatgt gtaaaccgtg
     8821 cacaggcgtg ttcttacgga gcggcacttt catttatctg tacgcgaact tctattcctg
     8881 cgtcatgaca ggccagcagc cactgcgcca cttccagcgg atcgccctcc cggcgtacca
     8941 ccctgccttc tttattccat aactgcagac aggtgctgcc gtcgagacgc accacaaaat
     9001 ccccacggca ggcctgatag gggtccgctt ggaattcgga aaataaacca cgttaagact
     9061 attttttagt caaaggactt tcaggaatcg ccagaatgtc gggacaacag cggttttggt
     9121 gtccgaaatc gtacaattct gtacatctac gcaagggaat gtacggaagt aagccaaacg
     9181 gttatcggac ggccggaaca tttcgtacag cctttacgtt cagtgagtta gctggcctgg
     9241 atgtaaccac tcgttcaact caattctctt agcgtgggaa aatttccctt ttccaagcgg
     9301 acccctatta atatttgttt tgttttaggt tctattcaac ataggtcatc ctaaaataca
     9361 ttgaagataa caagataaca agataacaag ataacaagat aacaaggtgt tatctttgtt
     9421 ttacttctgc tatcaatcgt gatacctctg tttttgatag tgttttaagt gcttctaccg
     9481 ccgccctgac aacatcagaa cgattcactc tgcctttacc tgaaaccatc tcagccgtta
     9541 taagggcatc aatgtctttt aggttttctt caaaaaaaga aatactcact ggttttgctt
     9601 tgcttacaac cgatgattta tctgatgact ttttggtatt atcaaggcca gccgtgccac
     9661 cgttaataaa tgctgcctcg tcacgaggtg aagtgtttgc tctatttctc acagaataac
     9721 ctctttcact aacgcttcga tttcttgctg ggctttatca ttttgcgttt caaaaattgt
     9781 cattgattcg gcccaagcat ctcgatgtgc tttacgatca catacccggg agtttgccag
     9841 atgcatttca gggtaatcct taagtacttc agctgcctca ttggcttcat taacaaacat
     9901 atttgtgggc gtcatgttta aaacgagata tccccgaaca tcctcattaa aatcttttgc
     9961 tgctgtaaaa acggagcatg tatgaggcac aacatccagg tccatctgag atgggcgcaa
    10021 aggagaaatg aatacatctg cggccatcaa tccactacgc atttctgcgc tatcacggcc
    10081 agcacagtct gcaataacaa aatcatagga tttttcgtgt tcctttaaca tggctttgat
    10141 gttacctgtc gcgcctgtaa ccggaatatg agggagatct tcagggcgat tgttgtacca
    10201 ggttaatact gatttctggt catctgcgtc gacaataatc actctattgc ctagtgacat
    10261 cagatacgca gctatagata ccgccagtgt acttttacct accccaccct tttgactacc
    10321 taatactatt atcattatag ccccatatta agttgttaag ttaacaactt aatattgcca
    10381 tctgaacgcg aatcatgcaa ttatttttgt tatattaaca acttaataag ttgttaagta
    10441 tttgatttat agattaatta agataacaag ataataagat aacaagataa caagataaca
    10501 agataacaag ataacaagat aacaagatag caatattatg ggtggattac atattcataa
    10561 ctgggctact ggcaaatctg tccatctggt ggtatacgca ggagaaagca tttcccgttt
    10621 catttgccac tcaggggcgg ttcctcgccc cgcaaaccac actttccctt ttcccgactg
    10681 gttcagttca tcgagtgttt tcattaactt ttcactgttt ttacggggct ggatttcatc
    10741 aaataatccc ggctgtgcta tacccgatgg tgtgaaatca gccagcatga ctcctgcctt
    10801 catatagcgg tacccttcac gccagacatg gtttaaggcc ctgcatgcgg cggcaataat
    10861 gtcccggctg tcctgtgtgg gcaatgaaag cttttccaca gcggcattgc tgtaacaggg
    10921 ttcttttact gcaaaggggg atgtccgtac aaatgtcgtc acctgccggc aatactgacg
    10981 ttccccacgt agtttctctg cagcccgctc tgcatactga acaacagcct ggtgcatggc
    11041 atctttgtct gtgattcgtt caccaaaact gcgactacag acaatctgtt gttttgccgg
    11101 tggtgcttct tccagggata tgcaggactc gccgttgagt tcgcgtaccg tacgctcaag
    11161 aatgacgctg aagtttttcc gtatgaatgc cgtgttagcc tgcgccagct gcagtgctgt
    11221 gttaataccc agcgcattca gcttttccgt cagtctgcgt cctactcccc agacctcacc
    11281 aactggctgc agccccagta gcttcaagat ccgattacgg ttttctgccg tcagcgcgac
    11341 cacaccggaa aactgtggcc attgctttgt tgcccactgt gcacttttag ccagcgtttt
    11401 tgtaggcgca atgcccaccc ccatggtgag tcctgtccag ctctttacct gttccctgag
    11461 ctgatgacca aaaaactccg gagagatgca atgatttatc ccccgcaaat caatgaacat
    11521 ttcatcaatt gagtagggct caactgcggg agaaagcgac tccagaacag ccataacccg
    11581 ttggctcatg ctgtggtaca gcgcataatt gctggaaaat acatgtattt tcttctccag
    11641 gcgcatttgt ctcacctgaa acctgggctg ccccattctg atgccaaggg cttttgcctc
    11701 cgggctgcgc gcgatcacac agccatcgtt attgctgagt acgatgaccg gttcgttgcg
    11761 aaggtccggg cggaaaactt tttcacatga ggcgtagaaa ctgttgatat cagccagtgc
    11821 aaacatcagc gtaactcccg cgtcctgtgt atcacgtgag tgacaacacc aaaaatacag
    11881 atgttttccg gatacagtgt gcggaattcc gggctgtctg aaaccggctc cagtgccggg
    11941 cgtgggcgca acagcagtcg tttgacggtg aactcaccgt cgatctcagc gataacgatg
    12001 tccccgtgtt gtggtttttc gcccctgtct actaccagca gatcaccatt ctgcacgcca
    12061 gcctggttca tcgattcacc gctggcgcgc agaaagaagg tggctgcagg tctgctgatg
    12121 caatagctgt tcagatccag ttcctgctca gcataatcag tggcaggcga aggaaaaccg
    12181 gcctggcaac gatcggcaaa caatggtcgc acgtaactgt catcgcctga agggtcagcc
    12241 ggacggtgat aaacggtact c
//
LOCUS       DAZFQD010000151        12216 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.151, whole genome shotgun sequence.
ACCESSION   DAZFQD010000151 DAZFQD010000000
VERSION     DAZFQD010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12216
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.151"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(158..913)
                     /gene="mlaA"
                     /locus_tag="ACHKTW_004304"
     CDS             complement(158..913)
                     /gene="mlaA"
                     /locus_tag="ACHKTW_004304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HFO6465220.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADGLYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
     gene            1207..2139
                     /locus_tag="ACHKTW_004305"
     CDS             1207..2139
                     /locus_tag="ACHKTW_004305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFO6465221.1"
                     /translation="MDNDKIDQHSDEIEVESEEKERGKKIEIDEDRLPSRAMAIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHVELEGVPGSFLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLMRLWGVVLLGNILGTGIAAWAFEY
                     MPIFNEETRDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTTHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKAEARQKAERAENIKKNDKNPA"
     gene            2215..2289
                     /locus_tag="ACHKTW_004306"
     tRNA            2215..2289
                     /locus_tag="ACHKTW_004306"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2248..2250,aa:Arg,seq:cct)
     gene            complement(2731..3978)
                     /locus_tag="ACHKTW_004307"
     CDS             complement(2731..3978)
                     /locus_tag="ACHKTW_004307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide MFS transporter"
                     /protein_id="HFO6465222.1"
                     /translation="MALNIPFRNAYYRFASSYSFLFFISWSLWWSLYAIWLKGHLGLT
                     GTELGTLYSVNQFTSILFMMFYGIVQDKLGLKKPLIWCMSFILVLTGPFMIYVYEPLL
                     QSNFSVGLILGALFFGLGYLAGCGLLDSFTEKMARNFHFEYGTARAWGSFGYAIGAFF
                     AGIFFSISPHINFWLVSLFGAVFMMINMRFKDKDHQCVAADAGGVKKEDFIAVFKDRN
                     FWVFVIFIVGTWSFYNIFDQQLFPVFYSGLFESHDVGTRLYGYLNSFQVVLEALCMAI
                     IPFFVNRVGPKNALLIGVVIMALRILSCALFVNPWIISLVKLLHAIEVPLCVISVFKY
                     SVANFDKRLSSTIFLIGFQIASSLGIVLLSTPTGILFDHAGYQTVFFAISGIVCLMLL
                     FGIFFLSKKREQIVMETPVPSAI"
     gene            complement(4050..4964)
                     /locus_tag="ACHKTW_004308"
     CDS             complement(4050..4964)
                     /locus_tag="ACHKTW_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoimidazole riboside kinase"
                     /protein_id="HFO6465223.1"
                     /translation="MSAKVWVLGDAVVDLLPESDGRLLPCPGGAPANVAVGIARLGGT
                     SGFIGRVGDDPFGALMQRTLLTEGVDITYLKQDEWHRTSTVLVDLNDQGERSFTFMVR
                     PSADLFLETTDLPCWRHGEWLHLCSIALSAEPSRTSAFSAMTAIRHAGGFVSFDPNIR
                     EDLWQDEHLLRLCLRQALQLADVVKLSEEEWRLISGKTQNDRDICALAKEYEIAMLLV
                     TKGAEGVVVCYRGQVHHFAGMSVNCVDSTGAGDAFVAGLLTGLSSTGLSTDEREMRRI
                     IDLAQRCGALAVTAKGAMTALPCRQELE"
     gene            5180..6613
                     /gene="cscA"
                     /locus_tag="ACHKTW_004309"
     CDS             5180..6613
                     /gene="cscA"
                     /locus_tag="ACHKTW_004309"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HFO6465224.1"
                     /translation="MTQSRLHAAQNALAKLHEHRGNTFYPHFHLAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALAPGDDNDKDGCFSGSA
                     VDDNGVLSLIYTGHVWLDGAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFR
                     DPKVWREADTWWMVVGAKDPGNTGQILLYRGSSLREWTFDRVLAHADAGESYMWECPD
                     FFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDNGHDF
                     YAPQSFLAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEA
                     ESLRQQHQSVSPRTISNKYVLQENAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDN
                     QSERLVLWRYYPHENLDGYRSIPLPQRDTLALRIFIDTSSVEVFINDGEAVMSSRIYP
                     QPEERELSLYASHGVAVLQHGALWLLG"
     gene            complement(6621..7616)
                     /locus_tag="ACHKTW_004310"
     CDS             complement(6621..7616)
                     /locus_tag="ACHKTW_004310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005088955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFO6465225.1"
                     /translation="MASLKDVARLAGVSMMTVSRVMHNAESVRPATRDRVLQAIQTLN
                     YVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDILLAIEQTASEFGWNSFLINIFS
                     EDDAARAARQLLAHRPDGIIYTTMGLRHITLPESLYGENIVLANCVADDPALPSYIPD
                     DYTAQYESTQHLLAAGYRQPLCFWLPESALATGYRRQGFEQAWRDAGRDLAEVKQFHM
                     ATGDDHYTDLASLLNAHFKPGKPDFDVLICGNDRAAFVAYQVLLAKGVRIPQDVAVMG
                     FDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEGREGGRVTRIPCPLLIRCST"
     gene            7859..8077
                     /locus_tag="ACHKTW_004311"
                     /pseudo
     CDS             7859..8077
                     /locus_tag="ACHKTW_004311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            8095..9423
                     /gene="dsdA"
                     /locus_tag="ACHKTW_004312"
     CDS             8095..9423
                     /gene="dsdA"
                     /locus_tag="ACHKTW_004312"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008721 - D-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046416 - D-amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HFO6465226.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPGTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFPETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLDDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAQQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASGFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNTTHLVWATGGGMVPEEEMNQYLAKGR"
     gene            complement(9531..11069)
                     /gene="emrY"
                     /locus_tag="ACHKTW_004313"
     CDS             complement(9531..11069)
                     /gene="emrY"
                     /locus_tag="ACHKTW_004313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HFO6465227.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPGLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQETMGYNAIWAGLAYAHIGIMPLLNSPLIGRYGNK
                     IDMRVLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(11069..>12216)
                     /gene="emrK"
                     /locus_tag="ACHKTW_004314"
     CDS             complement(11069..>12216)
                     /gene="emrK"
                     /locus_tag="ACHKTW_004314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HFO6465228.1"
                     /translation="NKKHSNRRKYFSLLAVVLFIAFSGAYAYWSMELEDMISTDDAYV
                     TGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLANIVRQT
                     NKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTLISSKAA
                     LNAAIQAYKANKALIMNTPLNRQPQVVEAADATKEAWLALKRTDIKSPVTGYIAQRSV
                     QVGETVSPGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENVVFHGRV
                     TGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSMTATIDT
                     KNEDIAEMPELASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
BASE COUNT         3422 a         2972 c         2783 g         3039 t
ORIGIN      
        1 tttcaactcc tgaatatcgg aaatagtcag tgacgaacat cagtattttt acatcattac
       61 gtccgggtca ttgtcggttt atctcctttt acttgtggta attgagggat aacaacaaaa
      121 aaggtgagta ttgcgactca ccttttttat ttgtttctta ttcagaatcg atatctttta
      181 aatcatcctg aatcgcttgc gcgttcggat tttcctgcgg tttgagttcg ccgccattag
      241 cgatgaaatc atgacgctgg aagtacgctt cgcgcaccat aatataagga tcggacgact
      301 gacgcagcag accatcggaa tccaacaact gcgcacgagt ttctatccct tcaagcgtcc
      361 atttacccac agacatcggc caggtcagcc aggaaagaac cgggtaaaga ccatccgcca
      421 tatcaccacc gtcatcacgc agcgtgaagc taccgtagaa cggtaactgg acgtaaggcc
      481 cataacccac gccataatga ccaagcgtac taccgaagcg gtgaggttca gtccgttgca
      541 gtttcgggtt cgccatccct gcaacatcaa taaaaccgcc catccccaaa atggtgttca
      601 ggaaaaagcg ggtaaagtgg accatcccct gataagggtc gccctgcaag aagtagttaa
      661 ccatcaccgc aggttcttca aggttgccag taaagttgct caaaccgtta cgcgccggtt
      721 gcggaacata atcacgccag gcgacagcga ccggtcgaac aatatacggg tctaatacat
      781 tgaagttgaa gttgtacatg gtgcggttga acccttctaa cgggtcagaa cgcccttgct
      841 gatctgtacc ggaactcgca caccccacca gaagcgtagt tcccagagca agcgccgaca
      901 ggcgaagctt cataaatgtc tccctgtttt tttatggctt atgcagtttg ccatccatga
      961 cggaacgata ccgtatccgc ctgtttaggt gtgggcgatt gtaacagcac gtcaactgat
     1021 gtccagacgc cctgatttgc tgatttgatc atagcctggt aatcgccgcc ctgtaggcta
     1081 cttgattcta tagaaacaga aaaaggcaaa cgcagccttt tctacatttt cagagtaact
     1141 cccgccagtt gcgagcaaaa aagccgctac gctttagcta tacgtgctaa ttcaagagaa
     1201 gagaccatgg acaacgacaa aattgatcaa cacagcgacg aaattgaagt tgagagcgaa
     1261 gaaaaagagc gcggcaaaaa aatagaaata gatgaagacc gactcccctc ccgggcgatg
     1321 gcaattcatg agcatatccg ccaggatggt gaaaaagagc tggaacgcga cgcaatggcg
     1381 ctactgtggt cagccattgc ggcgggtctg tcgatgggcg cttcgctact ggcaaaaggg
     1441 atatttcatg tcgaactgga aggagtgcca ggcagcttct tactggagaa tctcggttat
     1501 acctttggtt ttattatcgt cattatggcc cgccagcaat tatttaccga aaacaccgtg
     1561 actgcggtac tacccgtcat gcaaaaaccg acaatgagca acgtcggctt acttatgcga
     1621 ttatggggcg tcgtgctgct gggtaatatt ctcgggacag gtattgctgc atgggcattt
     1681 gaatatatgc ctatcttcaa tgaagaaact cgcgatgcat ttgtcaaaat cggcatggat
     1741 gtgatgaaga acacccccag cgagatgttt gccaacgcga tcatttccgg ctggctgatc
     1801 gccactatgg tttggatgtt tcctgcagcg ggtgcggcaa agattgtggt gattattttg
     1861 atgacctggc ttattgcact gggtgacacc acccacatcg ttgtcggttc tgttgaaatc
     1921 ctctatctgg tgtttaacgg cacgctgcac tggagcgatt tcatctggcc cttcgcacta
     1981 cctactttag cggggaacat ctgcggcggc acctttatct tcgcgttaat gagtcatgca
     2041 cagattcgta acgacatgag caacaagcgt aaagcagaag cacgccaaaa agcagaacgt
     2101 gcggaaaaca ttaagaaaaa tgataaaaac ccagcataaa tggcgagggt ttaagcaatc
     2161 gagcggcagc gtacttaccc cgcagtccat tagcgggtat actcatgccg cattgtcctc
     2221 ttagttaaat ggatataacg agcccctcct aagggctaat tgcaggttcg attcctgcag
     2281 gggacaccag atacccttca aacgaaatct accttcaccc cgtaaaagat gggtttggca
     2341 gcacacttgc cttatatcta ctcattttta ctgcaacagg ttgaaatctc agcactgtca
     2401 gaaagcgctg atgactaaac agccctgagc cgggcgatgt aaccatcaca cagaatcctg
     2461 atagcgaaat atggcgtgac tcgatacttc actccgcaat gcattccttg atgaattcgc
     2521 aggaccgtga tacacgggac aggtcactga atgacgacaa tgtcctggaa atcagcgaac
     2581 cgcgcatctg aagtacattt gagcgactgt accagaacat gaatgaggcg tttggattag
     2641 gcgattatta gcagggctaa gcattttact attattattt tccggttgag ggatatagag
     2701 ctatcgacaa caaccggaaa aagtttacgt ctatattgct gaaggtacag gcgtttccat
     2761 aactatttgc tcgcgttttt tactcaagaa gaaaatgcca aatagcaaca tcaggcagac
     2821 aatacccgaa attgcgaaga aaactgtttg gtagcctgcg tggtcaaaga gtatcccagt
     2881 cggcgttgaa agcagcacaa tcccaagcga actggcaatt tgaaaaccaa tcagaaagat
     2941 cgtcgacgac aggcgcttat caaagtttgc cacgctgtat ttgaagacgg atatgacaca
     3001 aagtggaacc tcaatggcat gtaacaactt cactaatgaa ataatccagg ggttaacgaa
     3061 cagcgcgcag gaaaggatac gcaacgccat aatcacaact ccgataagta atgcattttt
     3121 tggccctacc cgattcacaa agaaaggaat aatcgccatg cacagcgctt cgagtaccac
     3181 ctggaatgag ttgagataac catacaggcg cgttcctaca tcgtgtgatt cgaataaacc
     3241 tgaataaaag acaggaaaaa gttgttgatc aaaaatgtta tagaaagacc acgtccccac
     3301 aataaatatg acgaaaaccc agaagtttcg atccttgaaa actgcgataa aatcctcttt
     3361 ttttacccct cccgcatctg ccgctacgca ctggtgatcc ttatctttaa aacgcatgtt
     3421 gatcatcata aatacagcgc caaatagcga gaccaaccag aagttgatat ggggactgat
     3481 actaaaaaat atgccggcaa agaacgcgcc aatagcatag ccaaaagatc cccaggcgcg
     3541 cgctgttcca tattcgaaat gaaaatttcg cgccattttt tcggtgaagc tatcaagcaa
     3601 accgcatccc gccagatacc ccaagccaaa aaatagcgcc cccagaatta gacctacaga
     3661 aaaattgctt tgcagtaacg gttcataaac gtaaatcata aacggtccgg tcaagaccag
     3721 gatgaaactc atacaccaga tgagcggttt cttcagaccg agtttatcct gaacgatgcc
     3781 gtagaacatc ataaatagaa tgctggtaaa ctggttgacc gaataaagtg tacctaattc
     3841 cgtccctgtc aaccctagat gtcctttcag ccaaatagcg tataacgacc accacagcga
     3901 ccaggaaata aaaaagagaa atgagtaact ggatgcaaaa cgatagtacg catttctgaa
     3961 tggaatattc agtgccataa ttacctgcct gtcgttaaaa aattcacgtc ctatttagag
     4021 ataagagcga cttcgccgtt tacttctcac tattccagtt cttgtcgaca tggcagcgct
     4081 gtcattgccc ctttcgccgt tactgcaagc gctccgcaac gttgagcgag atcgataatt
     4141 cgtcgcattt ctctctcatc tgtagataat cccgtagagg acagacctgt gagtaacccg
     4201 gcaacgaacg catctcccgc ccccgtgcta tcgacacaat tcacagacat tccagcaaaa
     4261 tggtgaactt gtcctcgata acagaccacc acaccttctg cacctttagt caccaacagc
     4321 atggcgatct catactcttt tgccagggcg catatatccc gatcgttctg tgtttttcca
     4381 ctgataagtc gccattcttc ttccgagagc ttgacgacat ccgccagttg tagcgcctgc
     4441 cgcaaacaca agcggagcaa atgctcgtct tgccatagat cttcacgaat attaggatcg
     4501 aagctgacaa aacctccggc atgccggatc gccgtcatcg cactaaatgc gctggtacgc
     4561 gaaggctcgg cagacaacgc aattgaacag agatgtaacc attcgccatg tcgccagcag
     4621 ggcaagtctg tcgtctctaa aaaaagatcg gcactggggc ggaccataaa cgtaaatgaa
     4681 cgttcccctt gatcgttcag atcgacaagc accgtggatg tccggtgcca ttcatcttgc
     4741 ttcagatacg tgatatcgac tccctcagtt agcagcgttc tttgcattaa cgcaccaaaa
     4801 ggatcatccc ccacccgacc tataaaccca cttgttccgc ctaatctggc gattcccacc
     4861 gcaacgttag ctggcgcgcc gccaggacaa ggcagtaggc gcccgtctga ttctggcaag
     4921 agatctacga ccgcatcccc taaaacccat actttggctg acattttttt cccttaaatt
     4981 catctgagtt acgcatagtg ataaacctct ttttcgcaaa atcgtcatgg atttactaaa
     5041 acatgcatat tcgatcacaa aacgtcatag ttaacgttaa catttgtgat attcatcgca
     5101 tttatgaaag taagggactt tatttttata aaagttaacg ttaacaattc accaaatttg
     5161 cttaaccagg atgattaaaa tgacgcaatc tcgattgcat gcggcgcaaa acgccctagc
     5221 aaaacttcat gagcaccggg gtaacacttt ctatccccat tttcacctcg cgcctcctgc
     5281 cgggtggatg aacgatccaa acggcctgat ctggtttaac gatcgttatc acgcgtttta
     5341 tcaacatcat ccgatgagcg aacactgggg gccaatgcac tggggacatg ccaccagcga
     5401 cgatatgatc cactggcagc atgagcctat tgcgctagcg ccaggagacg ataatgacaa
     5461 agacgggtgt ttttcaggta gtgctgtcga tgacaatggt gtcctctcac ttatctacac
     5521 cggacacgtc tggctcgatg gtgcaggtaa tgacgatgca attcgcgaag tacaatgtct
     5581 ggctaccagt cgggatggta ttcatttcga gaaacagggt gtgatcctca ctccaccaga
     5641 aggaatcatg cacttccgcg atcctaaagt gtggcgtgaa gccgacacat ggtggatggt
     5701 agtcggggcg aaagatccag gcaacacggg gcagatcctg ctttatcgcg gcagttcatt
     5761 gcgtgaatgg accttcgatc gcgtactggc ccacgctgat gcgggtgaaa gctatatgtg
     5821 ggaatgtccg gactttttca gccttggcga tcagcattat ctgatgtttt ccccgcaggg
     5881 aatgaatgcc gagggataca gttaccgaaa tcgctttcaa agtggcgtaa tacccggaat
     5941 gtggtcgcca ggacgacttt ttgcacaatc cgggcatttt actgaacttg ataacgggca
     6001 tgacttttat gcaccacaaa gctttttagc gaaggatggt cggcgtattg ttatcggctg
     6061 gatggatatg tgggaatcgc caatgccctc aaaacgtgaa ggatgggcag gctgcatgac
     6121 gctggcgcgc gagctatcag agagcaatgg caaacttcta caacgcccgg ttcacgaagc
     6181 tgagtcgtta cgccagcagc atcaatctgt ctctccccgc acaatcagca ataaatatgt
     6241 tttgcaggaa aacgcgcaag cagttgagat tcagttgcag tgggcgctga agaacagtga
     6301 tgccgaacat tacggattac agctcggcac tggaatgcgg ctgtatattg ataaccaatc
     6361 tgagcgactt gttttgtggc ggtattaccc acacgagaat ttagacggct accgtagtat
     6421 tcccctcccg cagcgtgaca cgctcgccct aaggatattt atcgatacat catccgtgga
     6481 agtatttatt aacgacgggg aagcggtgat gagtagtcga atctatccgc agccagaaga
     6541 acgggaactg tcgctttatg cctcccacgg agtggctgtg ctgcaacatg gagcactctg
     6601 gctactgggt taacataata tcaggtggaa caacggatca acagcgggca agggatccgc
     6661 gtcactcttc ccccttcacg accttcaata atatgcaatg cagcttcccg cccgataatg
     6721 tcatgtggaa gctgaattgt ggtcagcggc ggtaaaaaca gatgcccgac gccaaccaga
     6781 ttatcaaagc ccattacggc gacatcctgc gggatacgta cccccttcgc caggagaacc
     6841 tgataagcga caaaggctgc gcgatcgtta ccacatatca gaacatcaaa atctggtttg
     6901 cccggtttga agtgggcatt gagtaaactt gcgagatcgg tgtagtgatc atcacctgtt
     6961 gccatgtgaa attgtttcac ctcagccaga tctcgtccag catcacgcca ggcctgctca
     7021 aatccctgcc gacgataccc tgttgccaac gcactttccg gtagccagaa gcataacggt
     7081 tgacgatagc ccgccgcgag caaatgctgt gttgattcat attgtgcagt gtaatcatca
     7141 gggatataac tgggtaacgc tgggtcatcc gccacacagt tcgccaatac aatattttca
     7201 ccatacagag actcaggcag cgtgatatgt cgcagcccca ttgtagtata gataatgcca
     7261 tccggacggt gggcaagcag ctgacgtgcc gcgcgggcag cgtcatcttc agaaaaaata
     7321 ttgattaaaa aactattcca gccgaactcg ctggcggttt gctcaatggc aagcagaata
     7381 tcaacagaga aaggagtggt agccgtgtcc tgcgccagca cggcgagagt cgacggctta
     7441 cgtccttgag cgcgcatctt acgggcggaa agatcaggaa cataattcag ggtctggatt
     7501 gcctgcaata cgcggtcacg cgttgcagga cgcacagatt ctgcattatg catcacccgg
     7561 gagactgtca tcatcgacac tcccgccagg cgtgcgacat cctttaatga agccataccc
     7621 aagccgtttg ccgtaaaacg ggcactgtag cagaaacaga cgtcactggc gagatccaac
     7681 gatattctca acgccctatc acccaacacg gcaatacaat aaaaaataac aataattgcc
     7741 ggataattgt actcaattcc gcctctgttc tcgccaacga gtaaccatat aggcaagtaa
     7801 gtaaaacatg ccaactggca tcgccaacct ggagaagaaa atataaatat ggatcattgt
     7861 gctgccgctg tatcccgaca tcagcccgga aattattgcg attgctatcg gttcaggtgc
     7921 aattggctgc acgatcgtta cggactcgct tttctggcta gtgaagcaat attgcggcgc
     7981 tacgctcaat gaaacattta aatactatac gacagcgaca tttatcgctt cagtcgtcgc
     8041 tctggcgggc acattcctgc tgtcatttat catctaagcg caaagagacg tactatggaa
     8101 aacgctaaaa tgaactcgct catcgcccag tatccgttgg taaaggatct ggttgctctt
     8161 aaagaaacca cctggtttaa tcctggcacg acctcattgg ctgaaggttt accttatgtt
     8221 ggcctgaccg aacaggatgt tcaggacgcc catgcgcgct tatcccgttt tgcaccctat
     8281 ctggcaaaag catttcctga aactgctgcc actgggggga ttattgaatc agaactggtt
     8341 gccattccag ctatgcaaaa acggctggaa aaagaatatc agcaaccgat cagcgggcaa
     8401 ctgttactga aaaaagatag ccatttgccc atttccggct ccataaaagc acgcggcggg
     8461 atttatgaag tcctggcaca cgcagaaaaa ctggctctgg aagcggggtt gctgacgctt
     8521 gatgatgact acagcaaact gctttctccg gagtttaaac agttctttag ccaatacagc
     8581 attgctgtgg gctcaaccgg aaatctgggg ttatcaatcg gcattatgag cgcccgcatt
     8641 ggctttaagg tgacagttca tatgtctgct gatgcccggg catggaaaaa agcgaaactg
     8701 cgcagccatg gcgttacggt cgtggaatat gagcaagatt atggtgttgc cgtcgaggaa
     8761 ggacgtaaag cagcgcagtc tgacccgaac tgtttcttta ttgatgacga aaattcccgc
     8821 acgttgttcc ttgggtattc cgtcgctggc cagcgtctta aagcgcaatt tgcccagcaa
     8881 ggccgtatcg tcgatgctga taaccctctg tttgtctatc tgccgtgtgg tgttggcggt
     8941 ggtcctggtg gcgtcgcatt cgggcttaaa ctggcgtttg gcgatcatgt tcactgcttt
     9001 tttgccgaac caacgcactc cccttgtatg ttgttaggcg tccatacagg attacacgat
     9061 cagatttctg ttcaggatat tggtatcgac aaccttaccg cagcggatgg ccttgcagtt
     9121 ggtcgcgcat caggctttgt cgggcgggca atggagcgtc tgctggatgg cttctatacc
     9181 cttagcgatc aaaccatgta tgacatgctt ggctggctgg cgcaggaaga aggtattcgt
     9241 cttgaacctt cggcactggc gggtatggcc ggacctcagc gcgtgtgtgc atcagtaagt
     9301 taccaacaga tgcacggttt cagcgcagaa caactgcgta ataccactca tctggtgtgg
     9361 gcgacgggag gtggaatggt gccggaagaa gagatgaatc aatatctggc aaaaggccgt
     9421 taataacgtt tcaacgcagc atcgcaatcc tttccctggg tgagcgatgc tgccgatggc
     9481 gcagacttaa gatccccggt cttacccgct ataaccccct ccttttatca tcacccaacg
     9541 cctttcgctg taaacggcgg tttcgcaaac caaaccaaaa ccgtaagtaa gataaaagca
     9601 atagccgcca tacggaaaat ctcatttgca gaaatagaaa gtgactgctg ggttatttca
     9661 ttattaattt cattaagaac tcctgaaagc gaaccatagt atttatccat aatttgtgac
     9721 gatgaattaa acacggggtt aaattgatcg atggttgctg tcaactgact atggtgtaac
     9781 gattcgcgtc gtccccacag cgtcattgtc aacgacgtac caactgatcc tgacaaggta
     9841 cgaaaaaaat tactcatact cgaggcattg gcaaatttat tatctggcaa gcctgaaaac
     9901 gaaatcgttg ttaagggtaa aaagaaacag gcaacggcga atccctggaa aaactgtggc
     9961 aaaatgatgc ctgtaaaatc aatcgttggc ataaatgtca cagaacgcca gtaatagcaa
    10021 accgcataca tcaaaaaact gaatgtcact aacacccgca tgtctatttt attgccataa
    10081 cgtcctatca aaggtgaatt caatagtggc atgatgccga tgtgcgcata agcaagcccg
    10141 gcccatatcg cattataccc catcgtttcc tggagtaact gcggcataag gacgatcgct
    10201 ccagagtaaa ataaatacgc gcaggtgata ctcacaatac caatggtgaa attacgagac
    10261 ttaaacaaac tgagatcaag aatcggattc tctgaggtcg actcccaaat gactaaagag
    10321 atcagagaaa taactgatac tactgttaat attattattg tactcgagtt gaaccaatcc
    10381 agatcgcgcc ctttatcaag cataatttgc aagccaccaa caccgagcac taacagggtc
    10441 agtcctggta gattcatttt taccggtgaa gtttcagttt ctcttccttt aagtaaggtt
    10501 aagcataatg tcaggacgat aatccccata gggacattga ttaaaaatat ccaaccccag
    10561 ctaaagttat cgcaaatata accgcccaat atcggcccac atatcggagc gataatcacg
    10621 gtcattgacc ataatgccag agcaaatgtt cttttttctg gtggataatt cctcaataat
    10681 aaactctgtg acagtggaat taacggcccc gccattaatc cctgaacgac tctaaaaaat
    10741 atcagcacat caagattggt cgataaacta cacattaagg aagacagcga aaaaaaagtg
    10801 actgaaagta aaaataacct taattcgcct attctttgtg ccagcctgcc ggtaacaggg
    10861 atcgcaatgg catttgctac gccaaacgag gtgataaccc aggtgccttc gtctgttgat
    10921 gctcccagaa aaccagatat tgtcggtatt gcgacgttag aaatagtgga atccaacatt
    10981 tgcataaatg tcgctaatga caatgcaata gtgacgcacc ataacgtccc accggttaat
    11041 ggtgccggag ttgatttagt gattgccatt aaagttgtcc attatgcgaa ataatgttgc
    11101 taatttcttt ttctatcgga ctggtatcaa taactaaagc cttactggta taagccggca
    11161 tggaggtcac ggttgaagcc agctcaggca tctcggcaat gtcttcgttc ttcgtatcaa
    11221 tagttgctgt catcgataaa ccaatacgca agggatgttc catgagttct tttggatcaa
    11281 gagaaacttc aaccggtaca cgctgaacga ttttgatcca gttccctgtc gcattttgtg
    11341 caggtaataa ggagaacgca ttgccggttc ccatattgat ccctgtcacc cgaccatgaa
    11401 acacaacatt ttcaccataa agatcgctga taatattgac cgattgacca atccgtacat
    11461 ccgtaagttg tgtttcttta aagttggcat taacccacat ttgacgtgcc ggtacgacag
    11521 ccattaacga ttgtccgggg ctcactgttt cgccgacctg aacacttctc tgggcaatat
    11581 agccggtaac cggactctta atatccgtac gtttaagcgc caaccaggct tctttagttg
    11641 catccgccgc ttcaacgact tgtggctgac ggtttaatgg tgtgttcatt attaaagctt
    11701 tattcgcttt ataagcctgg atagcggcat tcaatgccgc tttgctactt attaacgtat
    11761 ctttggtatg ctccagcgtt tcttttgaaa taaccccctg cttcgctaac ggcactcgac
    11821 ggttataatc ttctaaagat tgttgatact gaatacgtgc tgaagcgact tcggcactgt
    11881 attgtttatc ctgtaagtat agtttattcg tttgccgaac aatatttgcc agattatttt
    11941 tagctttatt gagtgcgata gtggcatcag ttttatccag tgaaactaaa atgtcacctt
    12001 gtcgaacgta gttcgtatct ttatgattaa cgacagtgac actacctgag acttgtgcag
    12061 aaattggatc tgcattcccc gtgacatagg cgtcatctgt actaatcatg tcttctaatt
    12121 ccattgacca ataggcatag gcacctgaaa acgcaataaa taaaactacc gccaataaag
    12181 aaaagtattt tcttctgtta gaatgttttt tatttg
//
LOCUS       DAZFQD010000152        12189 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.152, whole genome shotgun sequence.
ACCESSION   DAZFQD010000152 DAZFQD010000000
VERSION     DAZFQD010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12189
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.152"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            311..1081
                     /gene="yjjP"
                     /locus_tag="ACHKTW_004315"
     CDS             311..1081
                     /gene="yjjP"
                     /locus_tag="ACHKTW_004315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HFO6465229.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNKGGWDGAVITFFASTAAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            1072..1545
                     /gene="yjjB"
                     /locus_tag="ACHKTW_004316"
     CDS             1072..1545
                     /gene="yjjB"
                     /locus_tag="ACHKTW_004316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HFO6465230.1"
                     /translation="MGVIEFLLALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGAI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISAVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            1652..2191
                     /gene="dnaT"
                     /locus_tag="ACHKTW_004317"
     CDS             1652..2191
                     /gene="dnaT"
                     /locus_tag="ACHKTW_004317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HFO6465231.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            2194..2931
                     /gene="dnaC"
                     /locus_tag="ACHKTW_004318"
     CDS             2194..2931
                     /gene="dnaC"
                     /locus_tag="ACHKTW_004318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HFO6465232.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            2980..3474
                     /gene="yjjA"
                     /locus_tag="ACHKTW_004319"
     CDS             2980..3474
                     /gene="yjjA"
                     /locus_tag="ACHKTW_004319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein YjjA"
                     /protein_id="HFO6465233.1"
                     /translation="MMKTVKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            3728..6019
                     /gene="opgB"
                     /locus_tag="ACHKTW_004320"
     CDS             3728..6019
                     /gene="opgB"
                     /locus_tag="ACHKTW_004320"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HFO6465234.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIAAFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQETLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDRCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTMWKGKTAFKDTVIDMARYKSNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(6158..7180)
                     /gene="lgoD"
                     /locus_tag="ACHKTW_004321"
     CDS             complement(6158..7180)
                     /gene="lgoD"
                     /locus_tag="ACHKTW_004321"
                     /EC_number="1.1.1.414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
                     /protein_id="HFO6465235.1"
                     /translation="MSTMNVLICQQPKELVWKQREIPIPGDNEALIKIKSVGICGTDI
                     HAWGGNQPFFSYPRVLGHEICGEIVGLGKNIADLKNGQQVAVIPYVACQQCPACKSGR
                     TNCCEKISVIGVHQDGGFSEYLSVPVANILPADGIDPQAAALIEPFAISAHAVRRAAI
                     APGEQVLVVGAGPIGLGAAAIAKADGAQVVVADTSPARREHVATRLELPLLDPSAEDF
                     DAQLRAQFGGSLAQKVIDATGNQHAMNNTVNLIRHGGTVVFVGLFKGELQFSDPEFHK
                     KETTMMGSRNATPEDFAKVGRLMAEGKITADMMLTHRYPFATLAETYERDVINNRELI
                     KGVITF"
     gene            7319..8233
                     /gene="lgoR"
                     /locus_tag="ACHKTW_004322"
     CDS             7319..8233
                     /gene="lgoR"
                     /locus_tag="ACHKTW_004322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HFO6465236.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSHLRECGVLTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTMINQR
                     QLRPGETFSELQLARAAGVSPVVVREYLLKFGRYNLIQSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENTRYAH"
     gene            8448..9809
                     /gene="lgoT"
                     /locus_tag="ACHKTW_004323"
     CDS             8448..9809
                     /gene="lgoT"
                     /locus_tag="ACHKTW_004323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO6465237.1"
                     /translation="MEKENITLDPRSSFTPSSSADIPVPPDGLVQRSTRIKRIQTTAM
                     LLLFFAAVINYLDRSSLSVANLTIREELGLSATEIGALLSVFSLAYGIAQLPCGPLLD
                     RKGPRLMLGLGMFFWSLFQAMSGMVHNFTQFVLVRIGMGIGEAPMNPCGVKVINDWFN
                     IKERGRPMGFFNAASTIGVAVSPPILAAMMLVMGWRGMFITIGVLGIFLAIGWYMLYR
                     NREHVELTAVEQAYLNAGSVNARRDPLSFAEWRSLFRNRTMWGMMLGFSGINYTAWLY
                     LAWLPGYLQTAYNLDLKSTGLMAAIPFLFGAAGMLVNGYVTDWLVKGGMAPIKSRKIC
                     IIAGMFCSAAFTLIVPQATTSMTAVLLIGMALFCIHFAGTSCWGLIHVAVASRMTASV
                     GSIQNFASFICASFAPIITGFIVDTTHSFRLALIICGCVTAAGALAYIFLVRQPINDP
                     RKD"
     gene            complement(9858..11522)
                     /gene="tsr"
                     /locus_tag="ACHKTW_004324"
     CDS             complement(9858..11522)
                     /gene="tsr"
                     /locus_tag="ACHKTW_004324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HFO6465238.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQSTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWAYYEALPRDPRQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVIFAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGIAFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAAREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TKAVAVFRIQQQQQQQRETSAVVKTVTPATPRKMAVADSGENWETF"
     gene            complement(11641..12087)
                     /gene="hpaR"
                     /locus_tag="ACHKTW_004325"
     CDS             complement(11641..12087)
                     /gene="hpaR"
                     /locus_tag="ACHKTW_004325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HFO6465239.1"
                     /translation="MHDSLTIALLQAREAAMSYFRPIVKRHNLTEQQWRIVRILAESP
                     SMDFHDLAYRACILRPSLTGILTRMERDGLVLRLKPINDQRKLYISLTKEGQALYNRA
                     QTQIEEAYRQIEAQFTAEKMQQLTHLLEEFIALGNSRQEDIPGDNE"
BASE COUNT         2971 a         3211 c         2957 g         3050 t
ORIGIN      
        1 aatcacacaa ccaatattta ttcaatgaaa tctgatggtt ttaacttgtt aacattaaaa
       61 aacgtgcccg gttatctatt atcctgattc gtaaaggcca aacaataaat tcattatagt
      121 gataatattc atgtgatagt ttgtttgcgg cgagagataa ttcgcttttt atcaccgagc
      181 ttgtcccgat aaccaggatt aatcagcgca attttgatct taacctaccg caaaacaacc
      241 gtgcttcatg atacaaatgg attctcatta tctgaacaat acgcagcacg tctatgacaa
      301 agggcgagtt atgcaaactg agcaacagcg agccgtaaca cggctttgta tccagtgtgg
      361 attatttctt ttgcaacatg gtgcggaaag cgcgttggtt gatgagcttt cctcacgact
      421 gggtcgggcg ttggggatgg acagcgtcga aagttctatc tcttcgaacg ccatagtgct
      481 gacaaccatt aaagatgggc aatgcctgac atcgacacgt aaaaatcacg atcgcggcat
      541 taatatgcat gtggtgactg aagtccagca cattgtgatt ctggcggagc accatctgct
      601 ggattacaaa ggcgtagaga aacgatttag ccaaattcag ccattacgtt acccaagatg
      661 gctggtagcc ttaatggttg gcctttcttg tgcctgtttc tgtaaactca ataaaggtgg
      721 ctgggatggt gccgtcatca ccttctttgc cagtacggcc gcgatgtata tccgccagct
      781 gctggcacaa cgtcatcttc atccacaaat caacttttgc cttaccgctt tcgccgccac
      841 caccatttcc ggattgcttt tgcaacttcc cactttcagc aatacaccca ctatcgcgat
      901 ggccgccagc gttctgctgc tggtgccggg atttccgttg attaatgccg tcgccgatat
      961 gtttaaaggc cacatcaata ccggactggc acgctgggcg atcgccagtc tgctgacact
     1021 ggctacctgc gtcggcgtag tgatggcact gacgatttgg gggctacgcg gatgggtgtg
     1081 atcgaatttc tgttagcgtt ggcgcaggat atgatcctcg ccgccattcc tgcggtcggc
     1141 tttgcgatgg tgttcaacgt tcccgttcgg gcgttacgct ggtgtgcgct gcttggcgcg
     1201 attggtcatg gttcacgaat gatcttaatg accagcgggc tgaatattga gtggtcaact
     1261 tttatggctt ctatgctggt cggcaccatt ggtattcaat ggtcgcgctg gtatctggcg
     1321 catccgaaag tgtttaccgt ggcggccgtt atccctatgt tcccgggcat atcggcttat
     1381 accgcaatga tttcggcggt aaaaatcagc cagttaggtt acagcgaacc gttgatgatt
     1441 accctgttaa ccaactttct tacagcttca tcgattgttg gtgcgttatc catcggtctt
     1501 tccattcctg gattatggtt gtaccgcaag cgccctcgcg tataaaattg cctcgcttaa
     1561 gcacacggat gagagacagc ctcctctcct ccgtgtgtta ctataaaagt tatctccctt
     1621 ctcgttcatc gttccatatt tgagaaacag tatgtcttcc agagttttga ccccggacgt
     1681 cgttggtatt gacgccctgg tacacgatca ccaaaccgtt ctggcaaaag ctgaaggcgg
     1741 tgtggttgcc gtatttgcta acaatgcccc ggcgttttat gccgtcacgc ctgcacgcct
     1801 ggctgaactg ctggcgctgg aagaaaagct ggcgcgtccg ggaagcgatg tcgctctgga
     1861 cgatcaactc tatcaggaac cgcaagccgc tcccgttgct gtccccatgg ggaaattcgc
     1921 catgtatccg gactggcaac ccgatgccga ctttatccgc ctagcggcgc tatggggcgt
     1981 ggcgctaaga gagccggtga ccaccgaaga actggcctca ttcattgcct actggcaggc
     2041 ggaaggtaaa gtctttcacc atgtgcagtg gcaacaaaaa ctggcgcgca gcctgcaaat
     2101 cggtcgtgcc agcaacggcg gactgccgaa acgagatgtg aatacggtca gcgaacctga
     2161 cagccaaatt ccaccaggat tcagagggta acgatgaaaa acgttggcga cctgatgcaa
     2221 cgcctgcaaa aaatgatgcc tgcccatatc aaacctgcat tcaaaacggg tgaagagcta
     2281 ctggcctggc agaaagaaca aggagcgatc cgctccgccg ctctcgaacg tgaaaatcgg
     2341 gcgatgaaaa tgcagcgcac ctttaaccgc tccggtattc ggccactgca tcagaactgc
     2401 tcctttgaga actatcgcgt tgagtgtgaa gggcagatga atgcgttaag caaagcgcgc
     2461 cagtacgtcg aagagtttga cggcaacatc gccagcttca tcttttctgg taagccagga
     2521 accggcaaaa accatctggc ggcggcaatc tgcaacgagc tgctgctacg cggtaaatcc
     2581 gtattgatca tcaccgtggc cgatattatg tcggcgatga aagatacctt caggaatagc
     2641 ggtaccagtg aagaacaact gcttaacgat ctgagcaacg ttgatctgct ggtgatcgat
     2701 gagatcggcg tgcagaccga atcgaaatac gaaaaagtga tcatcaacca gatcgtcgat
     2761 cgccgctctt cttccaaacg cccaaccggg atgctgacca acagtaatat ggaagagatg
     2821 accaagttgc tgggcgaacg cgtgatggac cgtatgcgcc tgggtaacag tttgtgggtg
     2881 atcttcaact gggatagcta ccgtagccgt gtaacaggta aagagtatta atccccgttt
     2941 ctggcggtat actgcgacac aattccacct tgagtaatga tgatgaaaac tgttaaacat
     3001 cttctgtgct gtgccattgc cgccagcgca ttaatttcca ccggggtgca tgctgcgtcc
     3061 tggaaagatg cgctctccag cgccgccagc gaacttggca accaaaacag cacgacacag
     3121 gaaggcggtt ggtcgctcgc gtcattaact aacttgctta gcagcgggaa ccaggcctta
     3181 agcgcagata acatgaacaa cgccgcaggc attctgcaat actgcgcgaa gcaaaagctg
     3241 gcgtcggtaa ccgatgccga aaacatcaag aaccaggtgc tggaaaagct gggcctgaac
     3301 agtgaagagc aaaaagaaga caccaactat ctggatggca ttcagggttt gctgaaaacc
     3361 aaagatggtc agcaactcaa tctggataac atcggaacga ctccgctggc agaaaaggtg
     3421 aaaaccaaag cctgcgatct ggtgttaaag caggggctga acttcatttc ctgaaccgcg
     3481 aaacgccgcg ttttagctgt tacccggctg acgcggctct ctcctgccct tttccctgct
     3541 cctggacggt tttccccctg ttacacggga agcgaccgcg atcaaaaaag tctgtttata
     3601 aactaattcc taacaacatc gcattttttt gacactagaa ttgcagcaat atggcgacgc
     3661 cattacattg tgcttctcaa aaagcttaaa cgttggctaa acggcccggt ttagtgagga
     3721 tcgcccgttg tcagaactac tctctttcgc cctttttctc gcctctgtgc tgatttacgc
     3781 atggaaagcg ggacgtaaca cctggtggtt tgcagccaca ttaacggtgc tggggctatt
     3841 tgtcgtttta aatatcaccc tgtttgccag cgactatttt actggcgatg gtattaacga
     3901 cgcggttctc tataccttaa ccaacagcct gaccggtgct ggcgtcagca aatacatcct
     3961 gccgggtatc ggcattgtgc tggggctgac agcggtgttc ggtgcgctgg gctggatcct
     4021 gcgccgtcgt cgccatcatc cgcaccattt tggttacagc ctgctggcgc tcttactggc
     4081 gctgggttcg gtggacgcca gcccagcatt tcgtcagata acggaactgg tgaaatccca
     4141 gtcacgcgat ggcgacccgg actttgcggc ttattataaa gagccgtcga aaactatccc
     4201 tgacccgaaa ctcaacctgg tttatatcta cggcgaaagt ctcgagcgga cctattttga
     4261 taacgaagct ttcccggatc tcacgcctga actgggcgcg ttgaaaaatg aaggcctgga
     4321 tttcagccac acgcagcagc tgccaggaac ggattacacg attgcgggca tggtggcttc
     4381 tcagtgcggc ataccgctgt ttgccccctt tgaaggcaac gcctccgcct ctgtctccag
     4441 cttcttcccg cagaacatct gtctgggcga tatcctgaaa aactcgggtt atcagaacta
     4501 tttcgtgcag ggcgcgaatc tgcgttttgc cggtaaagat gtgttcctga agtcgcatgg
     4561 cttcgaccac ttatacggct cagaagagct gaaaagcgtg gtggccgacc cgcactatcg
     4621 caacgactgg ggattctacg acgataccgt tctcgatgaa gcgtggaaaa agtttgaaga
     4681 gctttcccgc tcaggtcagc gattctcact gtttaccctg acagtcgata cccatcaccc
     4741 ggatggtttt atctcgcgta cctgtaaccg caaaaaatat gattttgacg gtaagccgaa
     4801 ccagtcattc agcgcggtaa gttgcagcca ggagaatatc gcggcgttta tcaacaaaat
     4861 caaagcgtca ccgtggttta aagataccgt catcgtcgtc tcttctgacc atttagcgat
     4921 gaacaacacg gcgtggaaat accttaataa gcaggatcgc aataacctgt tttttgtcat
     4981 tcgtggcgac aagccgcagc aagagacgct ggcagtgaag cgtaacacga tggataacgg
     5041 cgcgacggtg ctggacattc tcggtggcga taactatctc ggacttggtc gtagcagttt
     5101 atccgggcag tcaatgtcgg aaatcttcct caatatcaaa gagaaaacat tggcgtggaa
     5161 gccggatatc atccgcctgt ggaaattccc taaagagatg aaagagttca ccatcgacca
     5221 gcagaaaaac atgattgcct tctcgggtag ccatttccgt ttgccgctgc tgttgcgggt
     5281 ttcagacaag cgcgtagaac cgctgccgga aagtgaatac tcagcaccgc tgcgtttcca
     5341 gctggccgat ttcgctccac gcgacaattt cgtctgggtt gaccgttgct acaagatggc
     5401 acaactctgg gctccggaac tggcactctc caccgactgg tgtgtctcgc aggggcagct
     5461 tggcggtcag caaattgtcc agcatgttga taaaacaatg tggaagggca aaacggcatt
     5521 taaagatacg gtcatcgaca tggcgcgtta caaaagcaat gtcgatacgc tgaaaattgt
     5581 tgataacgat attcgctaca aagccgacag tttcatcttc aacgtcgccg gtgcgccaga
     5641 agaggtgaaa cagtttagcg ggatttcccg tccggagtcg tggggccgct ggtccaacgc
     5701 tcagctgggc gatgaagtaa aaatcgagta caagcatccg ctgccgaaga aatttgacct
     5761 ggtgattacc gccaaagcat acggcaataa cgccagccgt cctattccgg tacgcgtagg
     5821 caatgaagaa caaactcttg tgctgggcaa tgaagtaacc accaccacac tgcatttcga
     5881 taacccaacc gatgccgaca cactggtaat tgtgccgccg gaacctgtct caaccaacga
     5941 agggaatatc ctcggacact cgccgcgtaa gctcgggatc ggcatggtgg aaattaaagt
     6001 tgtggaacgt gaagggtaat ttctgactga tgtgtaggtc gcataaggtg cgacctacac
     6061 accttctccg gcctacaaaa catcattgag aaacagaaaa aaacagcgcc caacacttcc
     6121 ccggacgctg catggcaact ttaataatct cgccttatca gaaagtaatt acacccttaa
     6181 tcaactcacg attgttaatc acatcgcgct cgtaggtttc tgccagcgtg gcgaacggat
     6241 agcgatgggt taacatcatg tcggcagtga ttttcccttc tgccatcagt cgaccgactt
     6301 tagcgaaatc ttccggcgtg gcgttgcggc tgcccatcat cgtcgtttct tttttatgga
     6361 attccggatc ggagaactgc aactcacctt taaacaggcc gacaaatacc accgtgccgc
     6421 cgtgacgaat caaattcacg gtgttattca tcgcatgttg attacctgtc gcgtcgatca
     6481 ctttctgcgc cagcgaacca ccaaactgcg cccgtagctg cgcgtcaaaa tcttcggctg
     6541 acgggtccag taaaggtaat tccagacgcg ttgccacatg ttcacggcgc gccggactgg
     6601 tatccgccac caccacctgt gcgccatcgg ctttagcgat tgccgccgcg cccagaccga
     6661 ttggccccgc cccgaccacc agcacctgct cgccgggagc aatggctgcg cgacgcaccg
     6721 catgagcgct aatagcgaaa ggttcaatca atgctgccgc ctgcgggtca ataccgtctg
     6781 cgggcaaaat gttcgccacc ggcacgctca aatactcact aaaaccgcca tcctgatgca
     6841 cgccaatgac tgaaattttt tcacagcaat tggtacgccc gcttttacac gccgggcatt
     6901 gctgacaggc aacataaggg atcacagcaa cttgctgacc atttttaaga tcagcaatat
     6961 ttttacccag cccaacaatc tccccacata tttcatggcc taaaacacgt ggataactaa
     7021 aaaatggttg atttccaccc caggcatgaa tatcggtacc gcaaatcccg acagacttaa
     7081 tttttattaa tgcttcattg tcacccggaa taggtatctc gcgttgtttc cagactaatt
     7141 ctttcggctg ctggcaaatt aaaacattca tcgtagacat gattcggctc tccatttttt
     7201 gttgccttag atatcaatga aaatgaaaaa tggcagcgga acggaaactc tgttttgtga
     7261 aaacacacgc ataaaaatgt tttaaatcgg gttttaatgg actcacaagg aggaaaatat
     7321 gagtcgttca caaaatttac gccacaatgt gattaaccag gtcattgatg atatggcgcg
     7381 gggtcatatc ccttccccac ttccatcgca aagtgcgctg gcggaaatgt acaacatcag
     7441 ccgcaccaca gtgcgtcata ttctcagcca tttacgcgaa tgcggcgtct tgacgcaggt
     7501 tggaaacgac tatgttattg cgcgtaaacc tgaccacgat gacggtttcg cctgtactac
     7561 cgcttcaatg agtgaacaaa acaaagtgtt tgagcaggct tttttcacga tgataaacca
     7621 gcgccagtta cgcccagggg aaactttttc tgaactgcaa ctggctcggg ccgcgggtgt
     7681 cagcccggtg gttgtaagag aatatctttt aaaattcggc cgttataatc ttattcaaag
     7741 cgaaaagcgc ggccagtgga gcatgaagca attcgatcaa tcctacgccg agcagctgtt
     7801 tgagctacgc gaaatgctcg aaacacattc acttcagcac tttctcaacc tgcctgatca
     7861 tgatccccgc tggctgcagg cgaaaaccat gctcgaacgt catcggctac tgcgtgacaa
     7921 catcggcaat agctttcgca tgttctcgca acttgacaga gattttcact ccttactgct
     7981 ttcagctgcc gacaatatct tttttgatca atcgcttgag atcatctcag tcatctttca
     8041 ttttcactat caatgggacg aaagcgatct taaacagcgc aatatcattg ccgtcgatga
     8101 gcatatgacc attctcagcg ctctgatttg ccgcagtgac cttgatgcca cccttgcgct
     8161 ccgtaaccat ttgaattcag ctaaacaatc aatgattcga tccattaatg agaacacgcg
     8221 ttatgctcat taagtacgga ttaaaaacag caaactatta gcgggtgcac ggttttatca
     8281 aaagataaat accgggaaat tcccgcttac ctatgctcac aatcagtcga ctaaaaatgt
     8341 ttttgtgagc ggtagtaaag tcctaaaact ttaacctgcc gttaatgtca accttgcgta
     8401 attcacaatt acgtaaacaa ttaaaattat aaatacagga gtccggcgtg gaaaaagaaa
     8461 atatcaccct cgatccgcgt tcttcattta ctccatcttc gtcggcagat attcccgtgc
     8521 caccagatgg attagttcaa cgcagtaccc gaattaaacg cattcaaacc accgccatgt
     8581 tgttattatt ttttgcggcg gtaatcaatt atctcgaccg cagttcgctg tcggtagcaa
     8641 atttaacgat tcgtgaagaa ttgggattaa gtgccaccga aatcggcgct ttgctctccg
     8701 tgttttcact cgcttacggg attgcgcaac ttccttgcgg cccactattg gatcgtaaag
     8761 gcccacgcct gatgctggga ctggggatgt tcttctggtc actgttccag gcaatgtctg
     8821 gcatggtgca caactttacg cagttcgtgt tggtgcgtat cggtatgggg attggtgaag
     8881 cgccgatgaa cccatgcggt gtaaaagtca ttaacgactg gttcaacatc aaagagcgcg
     8941 gacgcccgat gggcttcttc aacgcagctt ctaccattgg cgttgccgta agcccaccga
     9001 ttctggcggc gatgatgctg gtgatgggct ggcgcgggat gtttattacc attggtgtac
     9061 tggggatttt tctcgccatc ggctggtata tgctctatcg caaccgcgag cacgtagaac
     9121 tgactgccgt tgaacaagct tatctcaatg caggtagcgt caatgcccgc cgagatccgc
     9181 tcagttttgc cgaatggcgc agcctgttcc gtaaccgtac aatgtgggga atgatgctcg
     9241 gattcagtgg catcaactac actgcgtggc tgtatctggc ctggcttcct ggttacctgc
     9301 aaacagccta taacctggat ttaaaaagca cagggttgat ggcggctatc cctttcctgt
     9361 ttggggctgc cgggatgctg gtcaacggtt acgttactga ctggctggtc aaagggggaa
     9421 tggctccgat taaaagccgt aagatctgca ttattgccgg gatgttctgt tctgccgcct
     9481 ttacgctgat agtaccacaa gcgacaacat ccatgacggc ggttctgctg attggcatgg
     9541 cactgttctg tattcacttt gccggaacat cctgctgggg cttgatccac gtcgcagttg
     9601 cttctcgcat gactgcgtcg gtgggcagta tccagaactt tgccagcttc atctgcgcct
     9661 cttttgcgcc gatcattact ggttttattg ttgataccac ccactcattc cgtctggcac
     9721 taatcatctg cggttgcgtc accgcagcgg gggcactggc gtacatcttc ctggttcgtc
     9781 agccgatcaa cgacccacgt aaagattaat ctccctatgc ccgataacat tttgcttatc
     9841 gggcattttt atggcgatta aaacgtttcc cagttctccc cgctatctgc cactgccatt
     9901 ttacgcggcg tagctggcgt cacggttttt accacagccg atgtttcacg ctgctgttgc
     9961 tgctgttgct gaatccggaa cactgccaca gctttggtca ggcgactggc ctgctcttcc
    10021 agcgcggcgg cggcggcggc agattcttcc accagcgcgg cgttctgttg agttacccgg
    10081 tccatctcag caaccgctaa gccaacctga tcgataccac ggctctgctc atcagaagca
    10141 gaagctattt cgcccataat gtccgtcacg cgggtcacag cactgacaat ctccgccatt
    10201 gtttccccgg cgctttcgac cagcgtagag ccaacatcca ctttccccac cgagtcttca
    10261 atcaggcttt taatttcacg agccgcctgg gcgctacgct gggccagatt acgcacttct
    10321 cccgcaacca ccgcaaaacc gcgcccttgc tcacccgcac gcgcagcctc aaccgccgcg
    10381 ttcaaagcca gaatattggt ctggaaggca atgccgtcaa ttacgctgat aatatcggcg
    10441 attttctgcg aactggtgga gatatcgcgc atagtctgca ccacgttatc caccactttg
    10501 ccgccgcgtt gcgccgtttc agaagcactt aacgccagat ggctggcctg gcgcgcattt
    10561 tcggcgttct gcttcaccgt tgcggtcagt tgctccatgc tggctgccgt ctcttccagc
    10621 gaagcggcct gttgctcggt gcgcgaagag agatcgttat tgccggtggc gatttcgctg
    10681 gcaccgctat agatggcatt ggccccgttg cgcacatcac cgacggtacg catcagctct
    10741 ccctgcatat ggcgcaaact ctctgccagt tgccccatct cattagagcc atccacctca
    10801 atcggtttca ccagatcgcc gcctgcaata tgacgaatgc tgtcaatcag gcgattcatt
    10861 ggcgctacca gcgaggcttt aataccgaac cagacggcga agatgaccgc cagtacgacg
    10921 atcatcacgc ccaccagaat ccacatagcc tggctgtagg aggcattgtt atcgctgacg
    10981 gcgatatcat agagccgatc gttttgctcc atgtaagcca catactgctt ctcgaaaccg
    11041 tcctgatatc cctgggtcgg ctgatcaaag aactcgttga ttttgcctgc acctaatagt
    11101 tggatcagct ccgccagcgc attgtgataa atatcgtaat tacgtttgat ctctgccgct
    11161 gcggcggtgc tctgacgcgg gtcacgcggc aacgcttcgt aatacgccca gtttttttcc
    11221 gcctgtttca gcgaaatact ggcactctgc atcagctcag caacggttga accgctacca
    11281 atattattct gatccatcat gtagcggata cccgcgcggt tgagggtgtt acgcgtctgc
    11341 aacaacgcga cccagctgcc attcagcgtg gattgctgct ggcgaatggt ttgtaaaaca
    11401 gtgaaatttt ctttgtcatt ctttaaggca ttaaagaaca gaccgcctga tgtcagttgt
    11461 aaaaggccaa aaacagccaa aaccagcagt aagctggtca cgatcttgat acgttttaac
    11521 atggtttctc tttcctgtgg accgatgcaa gattttcggc ctggaaagca aaaactttat
    11581 gaaaaggtgt aacccgccat ccatggaaaa accaggcgct ggttatcaga tactaaaaag
    11641 ttattcatta tcgcccggga tgtcttcttg tcgggaattg cccagagcaa taaattcctc
    11701 taacagatgg gttaactgtt gcattttctc ggcagtaaac tgagcttcaa tctgccgata
    11761 ggcttcttca atctgtgtct gagcgcggtt atacagcgcc tgcccctctt tggttagcga
    11821 gatatagagc ttccgctgat cattgatcgg ctttaatcgc aacactaaac cgtcgcgttc
    11881 catccgcgta aggatcccgg tcaggcttgg gcgcaaaatg caggcgcgat acgccagatc
    11941 gtgaaaatcc attgatgggc tttccgccag aatacgcacg atgcgccact gttgctcagt
    12001 caaattatgc cgtttaacga ttgggcgaaa ataactcatc gccgcttctc gcgcctggag
    12061 caacgcaatg gttagagagt cgtgcattat ctttcccctg aaaaattcaa aatcattaat
    12121 atagaaacag ttaatcatca cgttaccggg actgcgctgc ccctgcaatt cttcatttta
    12181 tcaaaattg
//
LOCUS       DAZFQD010000153        12144 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.153, whole genome shotgun sequence.
ACCESSION   DAZFQD010000153 DAZFQD010000000
VERSION     DAZFQD010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12144
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.153"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..553
                     /locus_tag="ACHKTW_004326"
     CDS             <1..553
                     /locus_tag="ACHKTW_004326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFO6465240.1"
                     /translation="VQTLNAANNQLKYINLDFLTYLPSIKSLRLDNNKITHIDTNNTS
                     DIGTLFPIIKQSKNLNFLNVSGKNNCPTMQLMLFNLFSPALKLNTGLAILSPGAFEVH
                     SDGIDVDNELFHYPIKKAYTPYNIHTYKTEEVVNQRNIKVKNMTLDEINNTYCNNDYY
                     NQAIREEPIDLLDRSFSSSSWPF"
     gene            877..2265
                     /gene="narU"
                     /locus_tag="ACHKTW_004327"
     CDS             877..2265
                     /gene="narU"
                     /locus_tag="ACHKTW_004327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
                     /protein_id="HFO6465241.1"
                     /translation="MALQNEKNSRYLLRDWKPENPAFWENKGKHIARRNLWISVSCLL
                     LAFCVWMLFSAVTVNLNKIGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGGRRW
                     TVFSTAILIIPCVWLGIAVQNPNTPFGIFIVIALLCGFAGANFASSMGNISFFFPKAK
                     QGSALGINGGLGNLGVSVMQLVAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAWIWV
                     PLLAIATIAAWSGMNDIASSRASIADQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFA
                     MLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTLINFIFMAIFSALLF
                     LTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRVKMKGGSDEQA
                     QKEAVTETAAALGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCVLLT
                     WLVYGRRKFSQK"
     gene            2347..6087
                     /gene="narZ"
                     /locus_tag="ACHKTW_004328"
     CDS             2347..6087
                     /gene="narZ"
                     /locus_tag="ACHKTW_004328"
                     /EC_number="1.7.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
                     /protein_id="HFO6465242.1"
                     /translation="MSKLLDRFRYFKQKGETFADGHGQVMHSNRDWEDSYRQRWQFDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWEIQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPLIRKRLIELWREALKQHSDPVLAWASIMNDPQKCLSYKQVRGRGGFIR
                     SNWQELNQLIAAANVWTIKTYGPDRVAGFSPIPAMSMVSYAAGTRYLSLLGGTCLSFY
                     DWYCDLPPASPMTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTIAITPDYSEVAKLCDQWLAPKQGTDSALAMAMGHVILKEFHLDNPSDYFINYCRRY
                     SDMPMLVMLEPRDDGSYVPGRMIRASDLVDGLGESNNPQWKTVAVNTAGELVVPNGSI
                     GFRWGEKGKWNLESIAAGTETELSLTLLGQHDAVAGVAFPYFGGIENPHFRSVKHNPV
                     LVRQLPVKNLTLVDGNTCPVVSVYDLVLANYGLDRGLEDENSAKDYAEIKPYTPAWGE
                     QITGVPRQYIETIAREFADTAHKTHGRSMIILGAGVNHWYHMDMNYRGMINMLIFCGC
                     VGQSGGGWAHYVGQEKLRPQTGWLPLAFALDWNRPPRQMNSTSFFYNHSSQWRYEKVS
                     AQELLSPLADASKYSGHLIDFNVRAERMGWLPSAPQLGRNPLGIKAEADKAGLSPTEF
                     TAQALKSGDLRMACEQPDSGSNHPRNLFVWRSNLLGSSGKGHEYMQKYLLGTESGIQG
                     EELGASDGIKPEEVEWQTAAIEGKLDLLVTLDFRMSSTCLFSDIVLPTATWYEKDDMN
                     TSDMHPFIHPLSAAVDPAWESRSDWEIYKGIAKAFSQVCVGHLGKETDVVLQPLLHDS
                     PAELSQPCEVLDWRKGECDLIPGKTAPNIVAVERDYPATYERFTSLGPLMDKLGNGGK
                     GISWNTQDEIDFLGKLNYTKRDGPAQGRPLIDTAIDASEVILALAPETNGHVAVKAWQ
                     ALGEITGREHTHLALHKEDEKIRFRDIQAQPRKIISSPTWSGLESDHVSYNAGYTNVH
                     ELIPWRTLSGRQQLYQDHPWMRAFGESLVAYRPPIDTRSVSEMRQIPPNGFPEKALNF
                     LTPHQKWGIHSTYSENLLMLTLSRGGPIVWISETDARELTIVDNDWVEVFNANGALTA
                     RAVVSQRVPPGMTMMYHAQERIMNIPGSEVTGMRGGIHNSVTRVCPKPTHMIGGYAQL
                     AWGFNYYGTVGSNRDEFIMIRKMKNVNWLDDEGRDQVQEAKK"
     gene            6084..7628
                     /gene="narH"
                     /locus_tag="ACHKTW_004329"
     CDS             6084..7628
                     /gene="narH"
                     /locus_tag="ACHKTW_004329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HFO6465243.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQIDDYYE
                     PFTFDYEHLHSAPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRNF
                     EAMQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCRG
                     WRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDA
                     DRIEEAASTEREVDLYERQCEVFLDPHDPSVIEEALKQGIPQNVIDAAQRSPVYKMAM
                     DWKLALPLHPEYRTLPMVWYVPPLSPIQSYADAGGLPKSEGVLPAIESLRIPVQYLAN
                     MLSAGDTGPVLRALKRMMAMRHYMRSQTVEGVTDTRAIDEVGLSVAQVEEMYRYLAIA
                     NYEDRFVIPTSHREMAGDAFAERNGCGFTFGDGCHGSDSKFNLFNSSRIDAINITEVR
                     DKAEGE"
     gene            7628..8323
                     /gene="narW"
                     /locus_tag="ACHKTW_004330"
     CDS             7628..8323
                     /gene="narW"
                     /locus_tag="ACHKTW_004330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HFO6465244.1"
                     /translation="MQILKVIGLLMEYPDELLWECKEDALALIRRDAPMLTDFTHNLL
                     NAPLLDKQAEWCEVFDRGRTTSLLLFEHVHAESRDRGQAMVDLLAEYEKVGLQLDCRE
                     LPDYLPLYLEYLSILPDDQAKEGLLNVAPILALLGGRLKQRKAPWYALFDALLQLAGS
                     TLSSDSVTKQVNSEERDDTRQALDAVWEEEQVKFIEDNATACDSSPLNQYQRRFSQDV
                     APQYVDISAGGEK"
     gene            8320..9000
                     /gene="narI"
                     /locus_tag="ACHKTW_004331"
     CDS             8320..9000
                     /gene="narI"
                     /locus_tag="ACHKTW_004331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HFO6465245.1"
                     /translation="MIQYLNVFFYDIYPYICATVFFLGSWLRYDYGQYTWRASSSQML
                     DKRGMVIWSNLFHIGILGIFFGHLFGMLTPHWMYAWFLPVAAKQLMAMVLGGICGVLT
                     LIGGAGLLWRRLTNQRVRATSTTPDIIIMSILLIQCLLGLSTIPFSAQYPDGSEMMKL
                     VGWAQSIVTFRGGSSEMLNGVAFVFRLHLVLGMTIFLLFPFTRLVHVWSAPFEYFTRR
                     YQIVRSRR"
     gene            9079..9972
                     /gene="yddE"
                     /locus_tag="ACHKTW_004332"
     CDS             9079..9972
                     /gene="yddE"
                     /locus_tag="ACHKTW_004332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family isomerase"
                     /protein_id="HFO6465246.1"
                     /translation="MKPQVYHVDAFTSQPFRGNSAGVVFPADNLSEAQMQLIARELGH
                     SETAFLLHSDDSDVRIRYFTPTVEVPICGHATVAAHYVRAKVLGLGNCTIWQTSLAGK
                     HRVTIEKHNDDYRISLEQGTPGFEPPLEGETREAIINALHLTEDDILPGLPIQVATTG
                     HSKVMIPLKPEVDIDALSPDLAALSAISKQIGCNGFFPFQIRPGKNETDGRMFSPAIG
                     IVEDPVTGNANGPMGAWLVHHNVLPHDGNVLCVKGHQGRALGRDGMIEVTVTIRDNQP
                     EKVTISGTAVILFHAEWAIEL"
     gene            complement(10068..10913)
                     /gene="nhoA"
                     /locus_tag="ACHKTW_004333"
     CDS             complement(10068..10913)
                     /gene="nhoA"
                     /locus_tag="ACHKTW_004333"
                     /EC_number="2.3.1.118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-hydroxyarylamine O-acetyltransferase"
                     /protein_id="HFO6465247.1"
                     /translation="MTPILNHYFARINWSGAAAVNIDTLRALHLKHNCTIPFENLDVL
                     LPREIQLDDQSLEEKLVIARRGGYCFEQNGVFERVLRELEFNVRSLLGRVVLSNPPAL
                     PPRTHRLLLVELEEEKWIADVGFGGQTLTAPIRLVSDLVQTTPHGDYRLLQEGDDWVL
                     QFNHHQHWQSMYRFDLCEQQQSDYVMGNFWSAHWPQSHFRHHLLMCRHLPDGGKLTLT
                     NFHFTHYENGHAVEQRNLPDVASLYAVMQEQFGLGVDDAKHGFTVDELALVMAAFDTH
                     PEAGK"
     gene            11086..11655
                     /gene="yddH"
                     /locus_tag="ACHKTW_004334"
     CDS             11086..11655
                     /gene="yddH"
                     /locus_tag="ACHKTW_004334"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HFO6465248.1"
                     /translation="MSRFIPIELHHASRLLNHGPTVMITSFDEQSQRRNIMAAAWSMP
                     VEFEPPRVAIVVDKSTWTRELIERNGKFGIVIPGVAATNWTWAVGSVSGREEDKFNCY
                     GIPVVKGPVLGLPLVEEKCLAWMECRLLPATSAQQKYDTLFGEVVSAAADARVFVEGR
                     WQFDDDKLNTLHHLGAGTFVTSGKRVTAG"
     gene            complement(11659..11886)
                     /gene="pptA"
                     /locus_tag="ACHKTW_004335"
     CDS             complement(11659..11886)
                     /gene="pptA"
                     /locus_tag="ACHKTW_004335"
                     /EC_number="5.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase PptA"
                     /protein_id="HFO6465249.1"
                     /translation="MPHIDIKCFPRELDEQQKAALAADITDVIIRHLNSKDSSISIAL
                     QQIQPESWQAIWDAEIAPQMEALIKKPGYSM"
     gene            complement(11985..>12144)
                     /locus_tag="ACHKTW_004336"
     CDS             complement(11985..>12144)
                     /locus_tag="ACHKTW_004336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HFO6465250.1"
                     /translation="GNAAELFSGIRHIAINILTNDKVFKAGLRRKMRKAAMDRNYLAS
                     VLAGRRLS"
BASE COUNT         2932 a         3112 c         3122 g         2978 t
ORIGIN      
        1 tgttcagaca ttaaacgctg ctaacaacca gttgaaatat ataaatcttg atttcctgac
       61 ttatcttcca tctatcaaaa gtttaagact ggacaataat aaaataaccc acattgatac
      121 taataataca tccgatattg gaactttatt ccccataata aaacagagca aaaacttaaa
      181 ttttttaaat gtttctggga agaacaattg ccctactatg cagctcatgt tatttaattt
      241 gttttcccca gcacttaagc ttaatactgg cctggcaatt ctttcgcctg gtgcatttga
      301 agttcactct gacggaatag atgtggataa cgaattgttt cactatccta ttaaaaaagc
      361 atatacccca tataatatac acacttacaa gacagaggaa gttgtaaacc agaggaatat
      421 aaaagttaaa aacatgacct tagatgaaat aaacaatact tactgtaata acgattatta
      481 caatcaggca ataagagagg aaccgataga ccttctggac agatcgtttt cctccagttc
      541 atggcctttt tagtgaataa atcatcacta attctataag tgtctggtct tgttgataat
      601 atagttaata tgtaaccctt tagattcacg atctgaaggg ttgaaacatg acatacgttc
      661 aaagattaac ccccgtttga aggttagctt gttagttaca tttagtaaca catatctggc
      721 tacttaatcc ccattctgcc cccgcggtta cgctttctta tctattcttt caatcagcac
      781 ttcacccaat tcatccttaa ttctaataac tggctattgc gccttacctc ctgttttgtc
      841 ttttttcatt accaatgtgt gcatgtgagg aacaatatgg cactgcaaaa tgagaaaaat
      901 agtcgttatc ttttacgcga ctggaaacca gaaaatccgg ccttctggga aaataaagga
      961 aaacatattg ctcgaagaaa cctctggata tcagtcagtt gtctacttct tgccttctgt
     1021 gtctggatgc tatttagcgc agttaccgtt aatctcaata aaatcggttt taattttact
     1081 accgatcaac tctttttatt aaccgcatta ccctccgttt ctggcgcatt attgcgtgtt
     1141 ccctactcct ttatggtgcc tatattcggt ggacgccgat ggacggtttt tagtactgca
     1201 atcctgatta ttccttgcgt ctggctcgga attgccgtgc aaaatccgaa tactcctttt
     1261 gggatattta tcgttatcgc tttgctatgc ggttttgccg gtgcaaactt tgcttcgagc
     1321 atgggcaata tcagtttctt ctttccaaaa gccaaacaag ggagcgctct tgggattaat
     1381 ggcggattag gaaacttagg tgtaagtgta atgcagctgg ttgcaccgct ggtcattttt
     1441 gtacctgtat ttgcctttct cggcgtcaat ggcgtaccgc aggccgacgg ttcggtgatg
     1501 tcgctggcga atgccgcatg gatttgggta ccgctactgg cgattgccac gatcgccgca
     1561 tggtcaggga tgaatgatat cgccagttca cgcgcctcaa ttgccgacca gctccctgtc
     1621 ttacaacgcc tgcatctctg gctgctgagc ctgctttacc ttgccacctt cggttcgttt
     1681 atcggttttt ctgcgggttt tgccatgctg gcaaaaaccc agttcccgga tgtgaatatt
     1741 ctgcgcctgg cgttctttgg cccatttatc ggtgccatcg cgcggtcggt tggtggtgct
     1801 atttccgata agttcggcgg cgtgcgggtg acgttgatca actttatttt tatggcgatt
     1861 ttcagtgccc tgctgttcct taccttaccg ggcacaggct ccggtaattt catcgccttt
     1921 tacgccgtat ttatggggct gtttctgact gcgggtctgg gaagtggttc tactttccag
     1981 atgatcgccg tcatctttcg ccagataacc atttatcggg taaagatgaa aggcggtagt
     2041 gatgagcaag ctcagaaaga agccgtcacc gaaacggcgg cggctctggg ctttatctca
     2101 gccattggcg cagtgggcgg cttttttatt ccgcaggcgt ttggcatgtc gctcaatatg
     2161 accggctctc cggtcggcgc gatgaaagtg tttttaatct tctacatcgt ttgtgtgctg
     2221 ctgacctggc tggtttatgg tcggcggaag ttcagccaaa aataagagat cgatgcctga
     2281 tgtacaaccg tgtcgcatcc gacaaggcat cagatcccaa ttcattcctg gagcaggagt
     2341 catgccatga gtaaactttt ggatcgcttt cgctacttca aacaaaaggg cgaaaccttt
     2401 gccgatggtc acggacaggt gatgcatagc aaccgcgact gggaggacag ctatcggcag
     2461 cgttggcagt tcgacaaaat cgtgcgttcc acccacggtg ttaactgtac aggctcctgt
     2521 agctggaaaa tctacgttaa aaatggtctg gtgacctggg aaatccaaca gaccgactac
     2581 ccgcgcactc gccctgacct gcccaatcat gaacctcgcg gctgcccgcg tggcgcaagt
     2641 tactcctggt atctttacag cgctaaccgc ctgaaatacc cgctcattcg taaacgactg
     2701 attgaactgt ggcgcgaagc cctcaagcaa cacagcgatc cggtactggc gtgggcatcg
     2761 attatgaacg atccgcaaaa gtgcctgagc tacaaacaag tgcgtgggcg cggcgggttt
     2821 atccgctcca actggcagga actaaaccag ctgattgccg ccgctaacgt ctggaccatc
     2881 aaaacctacg gcccggatcg cgttgccggt ttctcgccga tcccggcgat gtcgatggtt
     2941 tcttacgccg ccggaacgcg ttatctgtcg ctgcttggcg gcacctgttt aagtttctac
     3001 gactggtatt gcgacctgcc gcccgcctcg ccgatgacct ggggcgagca aaccgacgta
     3061 ccggaatctg ccgactggta taactccagc tacatcatcg cctgggggtc taacgtaccg
     3121 cagacacgta cgccggacgc ccacttcttt accgaagtac gctacaaagg cactaaaacc
     3181 atcgccatta cccctgacta ctctgaagtg gccaaattgt gcgaccagtg gctggcaccg
     3241 aaacaaggca ctgatagcgc cctggcgatg gcaatgggcc atgtgatttt aaaagagttt
     3301 catctcgata atcccagcga ctactttatc aactactgcc gccgctacag cgacatgccg
     3361 atgctggtaa tgctggagcc tcgcgacgat ggtagctacg ttcccgggcg catgatccgc
     3421 gcatctgacc tggtggatgg actgggcgaa agcaacaatc cgcagtggaa aaccgtagca
     3481 gttaataccg caggtgaatt ggtagtgccg aacggttcga ttggtttccg ctggggagaa
     3541 aaaggcaaat ggaatctgga atccattgcc gccggtacgg aaaccgaatt gtcgttaacc
     3601 ctgctcggtc aacatgacgc tgttgcaggc gtggccttcc cctactttgg cggcattgaa
     3661 aatccgcatt ttcgcagcgt aaaacacaat ccggtgctgg tgcgccaatt gcccgttaaa
     3721 aacctgacgt tagtcgatgg caacacctgt ccggtggtca gcgtttatga tttggtactg
     3781 gcgaattacg gcctcgatcg cgggctggaa gatgaaaaca gtgcgaaaga ttacgctgaa
     3841 atcaaaccgt acaccccagc ctggggtgag caaattaccg gcgtgccgcg ccagtatatt
     3901 gaaaccatcg cccgtgaatt tgccgatacc gcccataaaa cgcatgggcg ctcgatgatt
     3961 atcctcggcg caggtgttaa ccactggtat cacatggaca tgaactaccg cgggatgatc
     4021 aatatgctga tcttctgcgg ttgtgtcggg caaagcggtg gcggctgggc acactatgtc
     4081 ggccaggaaa aactgcgccc acaaaccggc tggttgccgc tggcctttgc gctcgactgg
     4141 aaccgaccac cacgccagat gaacagtacc tcgtttttct acaatcattc cagccaatgg
     4201 cgctatgaaa aagtctctgc tcaggagtta ctttcaccgc tcgccgatgc cagtaagtac
     4261 agcggtcatc tgattgattt caacgttcgc gccgaacgta tgggctggct accttctgcg
     4321 ccgcagctgg ggcgtaaccc gctcgggatt aaagctgaag ccgacaaggc cggattatcc
     4381 cccacagaat ttaccgccca ggcgctgaaa tcgggcgatt tacgtatggc ctgcgaacaa
     4441 ccagatagcg gcagcaatca tccgcgtaat ttgtttgtct ggcgctctaa cctgcttggc
     4501 tcctccggca aaggccacga gtatatgcag aagtatctgc tggggaccga aagcgggatt
     4561 cagggcgagg aactcggtgc cagcgacggg atcaaaccgg aagaagtcga gtggcaaact
     4621 gcggcgattg aaggcaagct cgacctgctg gtgacgctcg acttccgcat gtccagtacc
     4681 tgcctgttct ccgatatcgt tctgcccacc gccacctggt acgaaaaaga cgatatgaac
     4741 acctcggata tgcatccgtt tattcatccg ctttctgcgg cggtcgatcc ggcgtgggaa
     4801 tcacgcagcg actgggaaat ctacaaaggt atcgccaaag cattttcgca agtgtgcgtg
     4861 ggtcatcttg gcaaagaaac cgacgtggta ttacaacccc tactgcatga ctctccggca
     4921 gagctctcac agccgtgtga agtgctcgac tggcgcaaag gcgaatgcga tctgattcca
     4981 ggcaaaaccg cgccgaatat tgtggcggtg gagcgcgact accctgctac gtatgaacgc
     5041 tttacctcgc tcgggccatt gatggacaaa cttggcaatg gcggtaaagg gatttcgtgg
     5101 aatacccagg atgaaatcga tttcctcggc aaactcaatt acaccaagcg tgatggcccg
     5161 gcacaggggc gtccactgat tgacaccgct attgatgcat cagaggtcat tctggcactg
     5221 gctccagaaa ccaacggtca tgttgcagtc aaagcgtggc aggcgctggg cgaaatcacc
     5281 ggacgcgaac atacccatct ggcgctgcac aaagaggacg agaagattcg ctttcgcgat
     5341 attcaggcgc agccacgtaa aattatctcc agccccacat ggtccggtct ggaaagcgat
     5401 catgtttcct ataacgcggg atacaccaac gttcatgagt taattccgtg gcgcacgctg
     5461 tcgggacgcc agcaactcta tcaggatcat ccgtggatgc gagcttttgg tgaaagcctg
     5521 gtggcttatc gcccgcctat tgacacccgt agcgtcagtg aaatgcgcca aatcccgcca
     5581 aacggcttcc cggaaaaagc acttaacttc ctgacgccgc accagaaatg gggcattcac
     5641 tcaacctaca gtgaaaacct gctaatgctg acgctttctc gcggtggacc gattgtctgg
     5701 atcagcgaaa ccgatgcccg agaattgacc attgtcgata acgactgggt ggaagtgttt
     5761 aacgccaatg gcgcgctgac ggcccgcgcg gtggtcagcc aacgtgtacc gccgggtatg
     5821 accatgatgt atcacgccca ggaacgcatt atgaatatcc ctggttcgga agtaactggc
     5881 atgcgcggcg gcattcataa ctcggttacc cgcgtttgcc cgaaaccaac gcatatgatt
     5941 ggcggttacg cgcagctggc ctggggcttt aactactacg gcaccgtcgg ctcaaaccgt
     6001 gatgaattca tcatgatccg caagatgaag aacgttaact ggctggatga tgaaggtcgc
     6061 gatcaggtac aggaggcgaa aaaatgaaaa tacgttcgca agtcggcatg gtgcttaacc
     6121 tcgataaatg tatcggctgc catacctgtt cggtgacctg taaaaacgtc tggaccggac
     6181 gcgaaggcat ggagtacgca tggtttaaca acgtcgaaac caaaccgggc attggttatc
     6241 cgaaaaactg ggaagatcag gaagagtggc aaggcggctg ggtccgcgat gtgaatggca
     6301 agatacgccc gcgtctgggc agtaagatgg gcgtaataac caaaatcttc gccaacccgg
     6361 tggtgccgca gattgatgat tactacgaac ctttcacctt cgactacgaa catttgcata
     6421 gcgcaccgga aggcaaacat atccctactg ctcgcccgcg ttcgctgatt gacggtaagc
     6481 ggatggacaa agtgatctgg gggccaaact gggaagaact gctgggcggc gagtttgaaa
     6541 aacgtgcccg cgaccgcaac ttcgaggcca tgcaaaagga gatgtacggg cagtttgaaa
     6601 acaccttcat gatgtacctg ccgcgcctgt gcgaacactg cctcaatccc agctgcgtgg
     6661 cgacctgccc aagcggcgct atctacaaac gcgaagaaga cggcattgtg ctgattgatc
     6721 aggataaatg ccgtggctgg cgtttgtgca ttagcggttg tccgtacaaa aaaatctact
     6781 tcaactggaa aagcggcaag tcagaaaaat gtatcttctg ttacccacga attgagtccg
     6841 gtcaaccgac cgtttgctca gaaacctgcg tgggtcgcat ccggtatctg ggcgtgctgc
     6901 tttacgacgc cgaccgcatt gaggaagcgg caagcaccga gcgcgaggtt gacctctatg
     6961 aacgccagtg cgaagtgttc ctcgatccgc acgatccctc agtgatcgag gaagccctga
     7021 aacaaggtat tccacaaaat gtgattgacg ctgcccagcg ttcgcctgtc tacaaaatgg
     7081 cgatggactg gaaactggcg ctaccgttgc accctgaata ccgcaccctg ccgatggtct
     7141 ggtacgttcc tccgctgtca ccgattcagt cttacgctga tgcgggcggt ttgccgaaaa
     7201 gcgaaggcgt gctccccgcc atcgaaagcc tgcgtattcc ggtgcaatat ctcgccaata
     7261 tgttgagtgc cggtgatact ggtccggtac tgcgggcgct gaaacggatg atggcgatgc
     7321 gccactatat gcgttcacaa accgtggaag gcgttactga tactcgtgcc atcgacgaag
     7381 taggcctgag cgtcgcccag gtcgaagaaa tgtatcgcta tctcgccatt gccaactatg
     7441 aagatcgttt tgttatcccg accagccatc gggaaatggc gggcgatgcc ttcgcagaac
     7501 gcaacggctg cggttttacc tttggcgacg gttgccacgg ttcggacagt aaattcaacc
     7561 tgttcaacag tagccgtatc gatgccatca acatcaccga agtgcgcgac aaagcggagg
     7621 gcgaataatg cagatcctca aagtgatcgg cctgttgatg gagtatccgg acgagctgtt
     7681 gtgggaatgc aaggaggacg cgctggcgtt gatccgccgc gacgcgccga tgcttacgga
     7741 tttcacccac aacctgctta acgcgccgct gctggataaa caggccgaat ggtgcgaagt
     7801 gtttgaccgc gggcgcacca cgtcgctgct gctgttcgaa catgttcatg ccgagtcccg
     7861 cgatcgcggc caggcaatgg tggacctgct ggcggagtat gaaaaggtcg gcctgcagct
     7921 ggattgtcgg gaactgcccg attatctacc gctgtatctg gagtatttaa gcatactgcc
     7981 tgacgatcag gcgaaagaag gattgctcaa tgttgcgccg atcctcgccc tgcttggcgg
     8041 tcgcttaaaa caacgcaagg caccgtggta cgcgttgttt gatgctctgc tgcaactggc
     8101 aggaagtaca ctttcaagtg acagtgtcac aaaacaagta aacagcgaag agcgcgatga
     8161 cacccgccag gcgcttgatg cagtgtggga agaggaacag gtgaagttta ttgaagataa
     8221 cgccacggcg tgtgacagct cgccgcttaa tcaatatcag cgacgcttta gccaggatgt
     8281 cgcgccgcag tatgtcgaca tcagtgcggg aggtgagaaa tgattcagta tctgaacgtc
     8341 tttttttacg atatctaccc gtacatttgt gcgacggtgt ttttcctcgg cagctggctg
     8401 cgctacgact acgggcagta cacctggcgc gcctcctcaa gtcagatgct cgataaacgc
     8461 gggatggtga tatggtcgaa tctgttccat atcggcattt tggggatctt cttcgggcac
     8521 ctgttcggca tgttaacgcc gcactggatg tacgcgtggt ttttgccagt ggcagcgaaa
     8581 caactaatgg cgatggtgct cggtggtatt tgcggtgtgt tgacgttgat tggcggcgca
     8641 ggactgctgt ggcgcaggct gacaaatcag cgcgtgcgtg ccacttccac tacgccggat
     8701 atcatcatca tgagcatttt gctgatccag tgtctgttgg ggctaagtac cataccgttc
     8761 tcagcacagt atcctgatgg tagcgaaatg atgaagctgg tgggttgggc gcaaagcatt
     8821 gtcactttcc gtggtggatc gtcagagatg cttaacggtg tagcgttcgt cttccgcctg
     8881 catctggtgt tgggaatgac catcttcttg ctcttcccgt tcacccgact ggtgcatgtg
     8941 tggagcgccc cgtttgagta ctttactcgt cggtatcaga ttgtgcgctc gcggcgataa
     9001 tttcaccctg gccggatagc ccttcgctct ccggccactt gcaggctatg atagttctcc
     9061 agtcacagga gaacgttaat gaaaccgcag gtgtaccacg tcgatgcttt tacctcacaa
     9121 ccgtttcgcg gcaattctgc cggtgttgtt ttccccgccg ataatcttag cgaagcgcaa
     9181 atgcagctta tcgcccgcga gttaggccat tcggaaactg cttttctgct gcacagcgac
     9241 gacagcgatg tgcgcatccg ctactttacg ccaacggttg aagtgccgat ttgcggtcac
     9301 gcgacagtag ctgcgcacta tgtacgtgcg aaggtgttag gtttaggaaa ttgcacgatc
     9361 tggcaaacat cgctggcagg aaaacatcgc gtgactatcg aaaagcacaa cgatgattat
     9421 cgtatttcgc tggaacaagg tacgccgggc tttgagccac cgctggaagg tgaaacacgt
     9481 gaggcgatta tcaacgcgct tcatctcact gaagacgata ttttgccggg cttgccaatt
     9541 caggtcgcaa caacaggaca ctcaaaagtg atgatcccgt tgaaaccgga agtggatatc
     9601 gacgcccttt cgcctgatct tgctgcgctg agcgctatca gcaaacagat tggctgcaat
     9661 ggtttcttcc cgttccagat ccgcccaggc aaaaacgaaa ccgatggtcg tatgttctcg
     9721 cctgcgattg gtattgtgga agatccggtg actggcaatg ccaatggccc gatgggtgca
     9781 tggttggtac atcacaacgt attgccccac gatggcaacg tgttatgtgt taaaggccat
     9841 caggggcgcg cattggggcg tgacggcatg attgaagtga cggtgacaat tcgtgataac
     9901 caaccggaaa aagtcaccat ttcaggcaca gcggtgattt tgtttcatgc cgaatgggca
     9961 attgaactct gaaaccgttg cctgatgcgc ttcgcctgtc aggtctacaa ccgagctaat
    10021 gtatgccgga taaggcgttt acgccgcatc cggcaacctg acataaatta ttttcccgcc
    10081 tccgggtgcg tatcaaacgc cgccatcacc agcgctaact catccacggt aaagccatgt
    10141 ttcgcatcat ccacgcccag accaaactgt tcttgcatca cagcatataa tgacgccaca
    10201 tccggtagat ttcgctgctc caccgcgtgc ccattttcat aatgggtaaa atgaaaattg
    10261 gtcagcgtta gcttgccacc gtccggcaaa tggcggcaca tcagcaaatg atggcgaaaa
    10321 tgcgactgcg gccagtgcgc cgaccagaaa ttgcccatca cataatcgct ttgttgctgc
    10381 tcgcagagat caaaacggta catcgactgc cagtgctgat gatgattaaa ctgcaacacc
    10441 caatcatcac cctcctgcaa caaccgataa tctccgtgtg gcgtggtctg cacgagatcg
    10501 gaaactaaac gaatcggcgc ggttagcgtc tgcccaccga aaccgacatc agcaatccat
    10561 ttttcctctt ccagttccac caacagcaaa cgatgggtgc gcggcggtaa tgctggcgga
    10621 tttgataaca ctacgcgccc taacaagctg cgaacgttaa actccagctc gcgtaacacc
    10681 cgctcaaaca cgccattctg ctcaaaacag taaccgccac gacgggctat caccagtttc
    10741 tcttccagcg attgatcatc aagctgtatt tccctcggca gcaaaacgtc aaggttttca
    10801 aacggaatgg tgcaattgtg tttcaggtgc aatgcacgaa gcgtatcaat attgaccgca
    10861 gcagctcccg accagttaat acgggcaaaa tagtgattca gaatgggcgt catcaggcgt
    10921 ttcccttagt ggttttctca acttataacc tggaatagac gccgcaccgg ctattttgtg
    10981 cgatcgcgga catgcatcac gtttgcaaat cgccaatccc gttaatttcg ttacttattg
    11041 cgttacggac tacgcttaaa aaaaccttct tcgcgaggcg ataccatgag ccgattcatc
    11101 cccatagaat tacaccatgc cagccgtcta ttaaatcatg gcccaactgt catgatcacc
    11161 agtttcgatg agcaatccca gcggcgtaac attatggctg cagcctggtc aatgccggtg
    11221 gagtttgaac cgccacgcgt ggcgattgtg gtagataaat cgacatggac cagggagttg
    11281 attgaacgta acggtaagtt tggcatcgtt atcccgggcg ttgcagcaac taactggacg
    11341 tgggcagtgg gaagcgtgtc gggtcgtgaa gaagataaat ttaattgcta tggcattccg
    11401 gtggtgaaag gtccggtgct tggtttgcca cttgttgaag agaaatgcct ggcgtggatg
    11461 gagtgccgcc tgctacctgc gacttctgcg caacaaaaat acgacacgct gtttggcgag
    11521 gtggtatcag cagcggcaga cgcacgggta tttgtcgaag gccgctggca gtttgatgat
    11581 gataagctca atacgttgca tcatttaggt gctggcacgt ttgttactag cggcaagcgt
    11641 gttacggcag gttaagcatt acatgctgta accgggtttc tttatcaaag cctccatttg
    11701 gggcgcgatt tcggcatccc agatagcttg ccaagattct ggttgaatct gctgtagagc
    11761 aatgcttatc gaactgtctt tactgttcag atggcgaata ataacgtcgg taatatctgc
    11821 agcaagtgct gctttttgtt gttcgtccag ttcacgcgga aagcatttaa tgtcgatgtg
    11881 cggcatgatt acgctccttt tgaaagatgt cagtaaaatt aacacacatt gtgaatgtca
    11941 tgtgtccggt aaagcgttta tacgacaata atgccagggc aagattacga aagccttctc
    12001 cccgcaagga ctgacgccag gtagtttctg tccatggctg cttttcgcat cttacgtctt
    12061 aaccctgcct tgaatacctt atcattcgtc aaaatattaa tagcaatgtg ccgtatccct
    12121 gaaaataatt ctgctgcatt tcct
//
LOCUS       DAZFQD010000154        12118 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.154, whole genome shotgun sequence.
ACCESSION   DAZFQD010000154 DAZFQD010000000
VERSION     DAZFQD010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12118
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.154"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            79..1539
                     /gene="cls"
                     /locus_tag="ACHKTW_004337"
     CDS             79..1539
                     /gene="cls"
                     /locus_tag="ACHKTW_004337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFO6465251.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAR
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            1574..1903
                     /gene="yciU"
                     /locus_tag="ACHKTW_004338"
     CDS             1574..1903
                     /gene="yciU"
                     /locus_tag="ACHKTW_004338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HI1450 family dsDNA-mimic protein"
                     /protein_id="HFO6465252.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(1956..2960)
                     /gene="oppF"
                     /locus_tag="ACHKTW_004339"
     CDS             complement(1956..2960)
                     /gene="oppF"
                     /locus_tag="ACHKTW_004339"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppF"
                     /protein_id="HFO6465253.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(2957..3970)
                     /gene="oppD"
                     /locus_tag="ACHKTW_004340"
     CDS             complement(2957..3970)
                     /gene="oppD"
                     /locus_tag="ACHKTW_004340"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppD"
                     /protein_id="HFO6465254.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPEHELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            complement(3982..4890)
                     /gene="oppC"
                     /locus_tag="ACHKTW_004341"
     CDS             complement(3982..4890)
                     /gene="oppC"
                     /locus_tag="ACHKTW_004341"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HFO6465255.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTSGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            complement(4905..5825)
                     /gene="oppB"
                     /locus_tag="ACHKTW_004342"
     CDS             complement(4905..5825)
                     /gene="oppB"
                     /locus_tag="ACHKTW_004342"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HFO6465256.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            complement(5911..7542)
                     /gene="oppA"
                     /locus_tag="ACHKTW_004343"
     CDS             complement(5911..7542)
                     /gene="oppA"
                     /locus_tag="ACHKTW_004343"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HFO6465257.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLLYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            7826..8122
                     /locus_tag="ACHKTW_004344"
     CDS             7826..8122
                     /locus_tag="ACHKTW_004344"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6465258.1"
                     /translation="MRFLLANTFCKIVKQFSFIDRIRIYSLHPCLNKHLSIQSNRDWL
                     SMSIFSDYSSSSEMHNNLTIDYYLALSSTKGSGITNIISIILQQAQDYDVAKIT"
     gene            complement(8280..8927)
                     /gene="ychE"
                     /locus_tag="ACHKTW_004345"
     CDS             complement(8280..8927)
                     /gene="ychE"
                     /locus_tag="ACHKTW_004345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HFO6465259.1"
                     /translation="MIQTFFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            complement(8958..9011)
                     /gene="ychE"
                     /locus_tag="ACHKTW_004346"
     CDS             complement(8958..9011)
                     /gene="ychE"
                     /locus_tag="ACHKTW_004346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YchT"
                     /protein_id="HFO6465260.1"
                     /translation="MLSKGVLSARLFNLIYG"
     gene            9404..12079
                     /gene="adhE"
                     /locus_tag="ACHKTW_004347"
                     /gene_synonym="adhC"
     CDS             9404..12079
                     /gene="adhE"
                     /locus_tag="ACHKTW_004347"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFO6465261.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEGETAAKKEAAPAKAEKKAKKSA"
BASE COUNT         3170 a         3043 c         2867 g         3038 t
ORIGIN      
        1 tttcccgcgt ttttatttgc cagatgaatc catatgcgta aactcataac aatgcgcttt
       61 caaaaggatt tctaatctat gacaaccgtt tatacgttgg tgagttggtt ggccattctg
      121 ggatactggt tgctcattgc aggcgtaact ttacgcattc taatgaaacg acgcgcagtt
      181 ccctccgcga tggcctggct gttgattatt tacattctgc cgttagtcgg aattattgcc
      241 tatcttgccg ttggcgagct ccatttaggc aaacgccgcg ctgagcgcgc cagagcgatg
      301 tggccttcca ccgcaaaatg gcttaacgac cttaaagcct gtaagcatat cttcgccgaa
      361 gaaaatagca gtgtcgctgc gccattattc aagctttgcg agcgtcgtca ggggatcgct
      421 ggggtcaaag ggaatcagct acaactgatg accgagtcag atgatgtgat gcaggcgtta
      481 atccgcgaca tccagctcgc gcgccataat attgagatgg tgttttatat ctggcagccc
      541 ggcggcatgg cggaccaggt ggctgaatca ttaatggcgg ctgcacggcg tggtattcat
      601 tgccgattga tgctcgactc cgccgggagt gtggcttttt tccgcagccc gtggcccgag
      661 ctaatgcgta atgccggtat tgaagtggtc gaagccttaa aggtcaatct gatgcgtgtg
      721 tttttacgcc gtatggacct gcgccaacat cgcaagatga tcatgatcga taattacatc
      781 gcgtacaccg gcagcatgaa tatggtcgat cctcgctact tcaaacaaga tgcgggcgta
      841 gggcaatgga ttgatctgat ggcgcgtatg gaaggcccca tcgccaccgc gatggggatt
      901 atttattcct gcgactggga gattgaaacc ggaaaacgta ttctgccgcc accaccagat
      961 gtcaatatta tgccgtttga acaggccagc ggtcacacca ttcacacaat tgcttctggc
     1021 cccggctttc cggaagatct cattcaccag gcattattga ctgcggctta ttcggcgcgt
     1081 gaatatttga tcatgaccac gccctacttt gtgccaagcg atgatttact tcatgcgatt
     1141 tgcacggcgg cgcagcgcgg ggtggatgtc agtattatcc ttccgcgaaa aaatgactcg
     1201 atgctggtcg gctgggccag tcgcgcattc tttacggaac ttcttgctgc tggggttaaa
     1261 atttatcagt ttgaaggcgg gttactgcat accaagagcg tgctggtcga tggcgaacta
     1321 agtctggttg gcaccgttaa ccttgatatg cggagtctgt ggctgaattt cgagattacc
     1381 ctggcgatcg acgataaagg ttttggtgca gaccttgccg ccgttcagga cgattatatt
     1441 tcgcgttcac gtctgctcga tgcccgttta tggctaaaac gtccattatg gcaacgtgtc
     1501 gccgagcgac tgttttactt cttcagtccg ttgctgtaaa acgtcgccaa cagaggttaa
     1561 acaggtagtg actatggata tggatctaaa caatcgcctg actgaagatg aaacgcttga
     1621 gcaggcctac gatatttttc tcgaactggc tgccgacaat ctcgatccag ctgatgttct
     1681 gctgttcaat cttcagtttg aagagcgcgg cggcgcggag ttattcgatc cggcggaaga
     1741 ctggcaggaa catgttgatt tcgatttgaa cccagacttt tttgccgaag tggtgattgg
     1801 tctggcggac agtgaagatg gtgaaatcaa cgatgttttc gcccgcattc tgttatgccg
     1861 cgaaaaagat cacaaacttt gccatatcat ctggcgggaa taaataatcg cttttagcgt
     1921 gaaaaagggc tgacaactgt cagcccttat tgtgtttaaa gcggatcgac tttcaggcaa
     1981 gaaacggcgt gtctgaaact cccctccaga acaggacgtg ttttggcgca ctccggaccg
     2041 gcaatcgggc aacgggtacg gaaaacacaa ccggaaggcg ggttgatcgg cgacggtaat
     2101 tccccttcca gtaactggat ggttttgttc ttctccagat ccggatcagg tatggggact
     2161 gccgacatca atgccctggt gtaaggatgt agtggattgt ggtagacctc atcataggtc
     2221 cccagttcta ccgcatggcc gagatacatc accaacacac gatcggaaat gtgttttacc
     2281 acggccaggt catgagcgat aaaaattaat gacaatccca tctcacgttg cagctgctgg
     2341 agcaggttga ccacctgcgc ctgaattgac acgtccagcg ccgaaaccgg ctcatcgcag
     2401 ataatcagct tcggttcaag aataagagca cgagcaatcc cgatacgctg gcactgccca
     2461 ccggagaact catgcggata gcggttaatc aggttaggca ataacccgac tttcagcatc
     2521 atcgccttca cgcgctcgcg aacttcctgg cgtgacattt tcggatgata agtacgcagt
     2581 ggttcagcga tgatctcgcc gatggtcata cgcgggttta gcgatgccaa cggatcctgg
     2641 aaaatcatct gaatatcact gcgaacggca cgccattcat cgggcttcat gcccagcaac
     2701 tctttaccta accaggcaac atgaccgtcg gtcgccttga ccaaaccgat gatggcgcga
     2761 gcaaaggtgg acttaccgca tcccgattcc cctaccacac ctaatgtttc cccttcatac
     2821 aggcgaagag tgacaccatc gacggctttg agcgttttcg gcggttgcca gaaccactgt
     2881 ttgccatctt tgatttcaaa gtgcacttta agatcggcga tttcaaggag gacttttctt
     2941 ccttcagtta cagcattcat aacagttcct ccaccggttt aaagcaagca cgcagacggc
     3001 caggcgtaaa ctcttccagc ggcggtgcgc tactacaaat ttccatcgca tgtggacaac
     3061 gtggctggaa cgggcaacct ttcggtaatc gcagcaagtt tggcggatta cccgggatgg
     3121 tcaacattgt ttcaccttcc gcatcaagac gcggcaccgc gttgagcaaa ccgatagaat
     3181 aaggatgaac gggttgataa aagacatcgc gcgcattgcc atattccatc gtgcgcccgg
     3241 cgtacattac cagcactttg tcacagatcc ccgccaccac gccaagatcg tgggtgatca
     3301 taatgatggc ggtattaaat tcccgcttca gttcatttaa tagcgtcatg atctgcgcct
     3361 gtacggtgac gtccagcgca gtagtgggtt catccgcaat cagtagctta ggtcgacata
     3421 gcaatgccat cgcaatcatg actcgctgac gcatgccgcc agaaaattcg tgcgggtaca
     3481 ttttcatgcg tttacgcgct tccggcattt ttaccgcatc gagcatccgc accgactctt
     3541 caaacgcctc agctttgctc atgttcttat gcagcatcag cacttccatt aactgctcac
     3601 cgacgcgcat atagggattc aacgaagtca ttgggtcctg aaaaatcatt gagatttgtt
     3661 cagcgcgcag tttattgagt tcatgctctg gcaaattgag gatttcacgc ccattgaagg
     3721 ttgccgatcc gccaatacgt ccgttggcag ccaacaggcc catcaacgca aatgcagttt
     3781 gcgatttacc cgaaccagac tcacctacaa tacccagcgt ttctccggca cgtagggaaa
     3841 aattcaaatc attgaccgcc gtgacgtcgc cgtccggggt actaaaggtg acacgcaaat
     3901 ctttcacgtt cagcagtgcg tcagcctgtt gttgtgcgag cggcacagtt gcagtttcaa
     3961 ttacgctcat ggctgcactc cttaacgatc tttcgggtcg agggcatcac gcaagccatc
     4021 gccgataaag ttgaaacaaa acagcgtcac cacgaggaat cccgctggga acaacaataa
     4081 ccatggagag acttccatcg agttcgcgcc atcactcagc aatgcacccc agctgcttaa
     4141 cggctcttgc gtacccaacc ccaggaagct aaggaaagat tcaaagagga tcatgctggg
     4201 caccagaagt gatgcgtaga ccaccaccac accgagtaca ttcggcacaa tgtggcgaat
     4261 aacaatgccc gatgtcgata caccgccaac ttgtgccgcc tcaataaact ctttgcgctt
     4321 cagactcagg gtttgcccac gcacaatacg agccatatcc agccaggaaa ccatgcctat
     4381 cgccacgaaa atcagcagga tgttttgacc gaaaaaggtc accagcaaaa tgacgaagaa
     4441 catgaatggg aaggagttga ggatttccag cagacgcatc attaccgaat ccactttacc
     4501 gcccagataa ccggaaagcg aaccataaag tgtcccgacg accacagcca ccagtgccgc
     4561 agcaacaccg accatgagtg agatacgccc gccaatcgca acgcgcacaa gcaggtcgcg
     4621 accggatgag tcagtaccaa agtagtgacc ggactccata tccggggcgc tggacatcat
     4681 cgcccagtca gtatcgtcat aggcaaactg ggaaagcatc ggtgccagga ttacaaataa
     4741 cgcgatcagc accagcacta tcagactggc aaccgccgca cggttatgca taaaacgtcg
     4801 acgtgcgtcc tgccacaagc tgcgcccttc gacctccagc ttttcactga aattttccag
     4861 cgtctcgctg tttttcttac ttaacatcat cgcgagctcc agtatcagta acggattttc
     4921 gggtcgataa ccgcatatag cacatcgaca atggcattaa acaaaatggt taaagcacca
     4981 accaggatgg tcaggcttaa cactaaggaa tagtcacggt tcaatgcgcc attaacgaac
     5041 agttgtccaa tccccggcaa accataaatg gtttcgataa ccatagaacc ggtaataatg
     5101 ccgacaaatg cggggcccat ataagagagc acgggtaaca gagcaggttt taatgcgtga
     5161 cgtaaaatga tccgccgcat aggtaaccct ttcgcccggg cagtacgaat aaaattggag
     5221 tgtaatactt caatcataga gccacgggta atacgcgcaa tactggcgat ataagcgagt
     5281 gacaacgcca ccatcggcaa tatcatgaat ttaagagccc caccattcca gccaccgccc
     5341 ggcagccaat gcaaaatgat cgcaaatatc atgactaata atggcgcaac cacaaaactg
     5401 gggataacaa ccccggtcat tgccagcccc atcacggtat agtcccattt ggtgttttgt
     5461 tttaatgcgg caataacgcc agcactaaca cccagtatta ccgcaaggaa aaatgctgcg
     5521 gctcccagtt tggcagaaac gggaaaactg gatgcaacca ggtcattgac cgaataatct
     5581 ttatatttaa acgatggacc gaaatcaccg tgcgccagtt gtttcaggta actgaaatac
     5641 tgtgtcatga tcggatcatt aagatgatat ttcgcttcga tattggccat cacttccggc
     5701 ggtaaagtac gttcgccggt aaaagggctt cccggcgcga ggcgcatcat aaagaacgaa
     5761 atagtaataa gaataaatag cgtcggaatc gcttccagac agcgacgtag aataaatttt
     5821 aacattgccc taccttctgg cctgtgcctt ctgtaatgcg ataaaaatca gacaccgtgg
     5881 agcaggacac tcctgcccca cgtattgcca ttagtgcttc acaatgtaca tattccgggt
     5941 gtaggtatta tccagcggat ctttgccggt atagccacca acccacggtt tcaccagacg
     6001 cgcattcacg tagtaataaa caggaacaat ggccgaatcc ttatccagct gttgttctgc
     6061 tttagtgtac agagctgtgc gctgcgcctc gtcagtcact ttcagcgttt ccgccataat
     6121 gctgtcaaag gccgggctct tataatgcgc ggtattcatc gagctgttcg acagcatggt
     6181 gttcaggaag gaagttggtt cgttgtagtc tgcacaccag cctgcacggg ccacatcaaa
     6241 agtgccctgg tgacgggtgt cgaggaacgt tttccactcc tggttaacca gtttgacgtt
     6301 tacaccaatg tttttcttcc acaatgaaga ggcagcaatc gccagctttt tatgcagatc
     6361 ggaggtgtta tacagcaggt tgatggtcaa cggtttgtct gcggtataac ccgcttcagc
     6421 cagcagtttt ttcgcttctt cgttacgttt ttcctggctc cagccaaacc attctggctg
     6481 agtcaatttt gcgccatcag tatacggtgg agtgtaacca taggcgggca tgtcgccctg
     6541 cgctttcact ttattaacaa tgatatcgcg gtccatacct agtttcagtg cggtacgcac
     6601 acgcacatcg ttgaatggcg gtttctggtt gttaatttcg taatagtaag tgcacaggta
     6661 tggatcaacg tgaacttcgt ccgggatctc ttttttcagc ttctggaaca attcgatcgg
     6721 catgttgtta taagtcatgt cgatttcacc actacggtag cggttgacat cggtaacttc
     6781 agaagcaata ggcaaatagg ttacctggtt aataacggtt ttcgcgttgt tccagtaggt
     6841 cgggctgcgt tcaagaacga ttcgttcgtt tacgacccaa tcttttaagg tataggcacc
     6901 gttggtgacg atattaccag gctgggtcca tttttcgccg aatttctcga tagcggcttt
     6961 tggcaccggt gaagttgatg ggtgaacaag taatttatag aagtacggaa cgggttcact
     7021 taaggtgact tctaatgtgt gatcatcaat agctttcacg ccgagatcgg taatcggttt
     7081 tttcccttca agaatttcat caataccggc gatatgccca tattgcagat aactggcata
     7141 cggagaagca gtgttcggat caacagaacg ttgccagcta tacacaaagt cttgtgctgt
     7201 gactggcgta ccatcagacc atttcgcatc tttacgcaaa tggaaggtcc agactttcgc
     7261 gtctttatta tcccaggatt cagcgacgcc aggtgccgga tgaccgtcaa gatcgctgac
     7321 cagtaagcct tcaaacaggt ctcggctgat attagactcc ggaacacctt caattttgtg
     7381 cggatctaat gactgaactt ctgaaccatt gttacgtacc agcgtttgtt tttccgccag
     7441 tgtgacgcct gcgggtacat cagctgccag cgcgacattc cctgccatta gcgcagccag
     7501 aacgccagct gctactaaac ttctcttggt gatgttggtc attgtttttt ggactccctc
     7561 attataatta ctggtattaa ccagcatgtg taatcccgga ttgggaccct gcacaggctc
     7621 ggagactttc tgctgtcagg ttactttatc gttacggatt gctatcaccg actttattta
     7681 ttctggtgat tcagacgatc accttatgtc gattcgtact gtgtctcccc tgtcgagaca
     7741 cattattctt tcgcgacgct ctttaattgt taattattct cattaccaat aaagaatcgt
     7801 ctggcggttg cccggaaagt accaaatgcg gttcttactc gccaatacat tttgcaaaat
     7861 tgttaagcaa ttctctttta ttgaccgtat cagaatctat agcttgcatc catgccttaa
     7921 caaacatctt tcaattcaat caaatagaga ttggctaagc atgtctatct tttctgatta
     7981 cagttccagt agtgaaatgc acaataattt aacaattgat tattatttag cactttcctc
     8041 taccaaaggc agtggaatta ctaatatcat ttcaattatt cttcagcaag cccaggatta
     8101 tgatgtggcg aaaataacat gatggtgagg agaagcagtt aaggaaatcc ttataaccaa
     8161 ctgtaatttg agaagaatat ttttacctat tccttttgct tttgataact gcgcgggaac
     8221 aaagtaaaaa taatgacgaa gtaaacggag catggcagct ccgtttcatt tgaaaggaat
     8281 taattaagca ggccggggaa aatcccctta ataccagtaa cgataaattc aatccccaat
     8341 gccatcagca atagccccat aatacgcgta atcacgttga tgccggtctg gcgtaaaacc
     8401 cgtaccagcc acggtgccat gcggaacaat ccccagcaac ataaagcgaa caatgcaata
     8461 gccacaaaga aaccaaacag atagctaatg ctgtgataac gcgtacccca gacgatggta
     8521 gaactgatcg cccctggccc cgccatcaac ggcaacgcca atggcaccac accaatgctt
     8581 tcacgtaccg cggtttctga tttttcttgc ttgttctgtt tatcctcgcc aagcttgccg
     8641 ctgatcatcg acatcgctat cgtcaccacc aggatgcccc cggcgatacg gaacgaatca
     8701 attgatatac caaaaagttg cagaatcgtg tcgccgagaa aaagcgagat ccacaagata
     8761 atggccacag acaggttggc tgtaaggtta gttttgtttc gcgctgccgc tgtctgataa
     8821 ctggtcatgc tgataaagac gggaataatc cctaccgggt tgaccagcgc aaataacccg
     8881 atgaaaaatt tgaagtaaac gggaaaatca aaaaaggtct gaatcacggt tagctccgaa
     8941 gcaaaagccg gataatgtta gccataaata aggttgaaaa gacgcgctga caatacgcct
     9001 tttgacagca tttttcacct cctaactact taaaattgct atcattcgtt attgttatct
     9061 agttgtgcaa aacatgctaa tgtagccacc aaatcatact acaatttatt aactgttagc
     9121 tataatggcg aaaagcgatg ctgaaaggtg tcagctttgc aaaaatttga tttggatcac
     9181 gtaatcagta cccagaagtg agtaatcttg cttacgccac ctggaagtga cgcattagag
     9241 ataataactc taatgtttaa actcttttag taaatcacag tgagtgtgag cgcgagtaag
     9301 cttttgattt tcataggtta agcaaatcat caccgcactg actatactct cgtattcgag
     9361 cagatgattt actaaaaaag tttaacatta tcaggagagc attatggctg ttactaatgt
     9421 cgctgaactt aacgcactcg tagagcgtgt aaaaaaagcc cagcgtgaat atgccagttt
     9481 cactcaagag caagtagaca aaatcttccg cgccgccgct ctggctgctg cagatgctcg
     9541 aatcccactc gcgaaaatgg ccgttgccga atccggcatg ggtatcgtcg aagataaagt
     9601 gatcaaaaac cactttgctt ctgaatatat ctacaacgcc tataaagatg aaaaaacctg
     9661 tggtgttctg tctgaagacg acacttttgg taccatcact atcgctgaac caatcggtat
     9721 tatttgcggt atcgttccga ccactaaccc gacttcaact gctatcttca aatcgctgat
     9781 cagtctgaag acccgtaacg ccattatctt ctccccgcac ccgcgtgcaa aagatgccac
     9841 caacaaagcg gctgatatcg ttctgcaggc tgctatcgct gccggtgctc cgaaagatct
     9901 gatcggctgg atcgatcaac cttctgttga actgtctaac gcactgatgc accacccaga
     9961 catcaacctg atcctcgcga ctggtggtcc gggcatggtt aaagccgcat acagctccgg
    10021 taaaccagct atcggtgtag gcgcgggcaa cactccagtt gttatcgatg aaactgctga
    10081 tatcaaacgt gcagttgcat ctgtactgat gtccaaaacc ttcgacaacg gcgtaatctg
    10141 tgcttctgaa cagtctgttg ttgttgttga ctctgtttat gacgctgtac gtgaacgttt
    10201 tgcaacccac ggcggctatc tgttgcaggg taaagagctg aaagctgttc aggatgttat
    10261 cctgaaaaac ggtgcgctga acgcggctat cgttggtcag ccagcctata aaattgctga
    10321 actggcaggc ttctctgtac cagaaaacac caagattctg atcggtgaag tgaccgttgt
    10381 tgatgaaagc gaaccgttcg cacatgaaaa actgtccccg actctggcaa tgtaccgcgc
    10441 taaagatttc gaagacgcgg tagaaaaagc agagaaactg gttgctatgg gcggtatcgg
    10501 tcatacctct tgcctgtaca ctgaccagga taaccaaccg gctcgcgttt cttacttcgg
    10561 tcaaaaaatg aaaacggctc gtatcctgat taacacccca gcgtctcagg gtggtatcgg
    10621 tgacctgtat aacttcaaac tcgcaccttc cctgactctg ggttgtggtt cttggggtgg
    10681 taactccatc tctgaaaacg ttggtccgaa acacctgatc aacaagaaaa ccgttgctaa
    10741 gcgagctgaa aacatgttgt ggcacaaact tccgaaatct atctacttcc gccgtggctc
    10801 cctgccaatc gcgctggatg aagtgattac tgatggccac aaacgtgcgc tcatcgtgac
    10861 tgaccgcttc ctgttcaaca atggttatgc tgatcagatc acttccgtac tgaaagcagc
    10921 aggcgttgaa actgaagtct tcttcgaagt agaagcggac ccgaccctga gcatcgttcg
    10981 taaaggtgca gaactggcaa actccttcaa accagacgtg attatcgcgc tgggtggtgg
    11041 ttccccgatg gacgccgcga agatcatgtg ggttatgtac gaacatccgg aaactcactt
    11101 cgaagagctg gcgctgcgct ttatggatat ccgtaaacgt atctacaagt tcccgaaaat
    11161 gggcgtgaaa gcgaaaatga tcgctgtcac caccacttct ggtacaggtt ctgaagtcac
    11221 tccgtttgcg gttgtaactg acgacgctac tggtcagaaa tatccgctgg cagactatgc
    11281 gctgactccg gatatggcga ttgtcgacgc caacctggtt atggacatgc cgaagtccct
    11341 gtgtgctttc ggtggtctgg acgcagtaac tcacgccatg gaagcttatg tttctgtact
    11401 ggcatctgag ttctctgatg gtcaggctct gcaggcactg aaactgctga aagaatatct
    11461 gccagcgtcc taccacgaag ggtctaaaaa tccggtagcg cgtgaacgtg ttcacagtgc
    11521 agcgactatc gcgggtatcg cgtttgcgaa cgccttcctg ggtgtatgtc actcaatggc
    11581 gcacaaactg ggttcccagt tccatattcc gcacggtctg gcaaacgccc tgctgatttg
    11641 taacgttatt cgctacaatg cgaacgacaa cccgaccaag cagactgcat tcagccagta
    11701 tgaccgtccg caggctcgcc gtcgttatgc tgaaattgcc gaccacctgg gtctgagcgc
    11761 accgggcgac cgtactgctg ctaagatcga gaaactgctg gcatggctgg aaacgctgaa
    11821 agctgaactg ggtattccga aatctatccg tgaagctggc gttcaggaag cagacttcct
    11881 ggcgaacgtg gataaactgt ctgaagatgc gttcgatgac cagtgcaccg gcgctaaccc
    11941 gcgttacccg ctgatctccg agctgaaaca gatcctgctg gatacctact acggtcgtga
    12001 ttatgtagaa ggtgaaactg cagcgaaaaa agaagccgct ccggctaaag ctgagaaaaa
    12061 agcgaaaaaa tccgcttaat cagtagcgct gtctggcaat ataaacggcc ccttctgg
//
LOCUS       DAZFQD010000155        11970 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.155, whole genome shotgun sequence.
ACCESSION   DAZFQD010000155 DAZFQD010000000
VERSION     DAZFQD010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11970
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.155"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..2000)
                     /locus_tag="ACHKTW_004348"
     CDS             complement(<1..2000)
                     /locus_tag="ACHKTW_004348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HFO6465262.1"
                     /translation="MSKVFICAAIPDELATREEGAVAVATAIEAGDERRARAKFHWQF
                     LEHYPAAQDCAYKFIVCEDKPGIPRPALDSWDAEYMQENRWDEESASFVPVETESDPM
                     NVTFDKLAPEVQNAVMVKFDTCENITVDMVISAQELLQEDMATFDGHIVEALMKMPEV
                     NAMYPELKLHAIGWVKHKCIPGAKWPEIQAEMRIWKKRREGERKETGKYTSVVDLARA
                     RANQQYTENSTGKISPVIAAIHREYKQTWKTLDDELAYALWPGDVDAGNIDGSIHRWA
                     KKEVIDNDREDWKRISASMRKQPDALRYDRQTIFGLVRERPIDIHKDPVALNKYICEY
                     LTTKGVFENEETDLGTVDVLQSSETQTDAVETEVSDIPKNETAPEAEPSVEREGPFYF
                     LFADKDGEKYGRANKLSGLDKALAAGATEITKEEYFARKNGTYTGLPQNVDTAEDSEQ
                     PEPIKVTADEVNKIMQAANISQPDADKLLAASRGEFVEGISDPNDPKWVKGIQTRDSV
                     NQNQHESERNYQKAEQNSPNALQNEPETKQPEPVAQQEVEKVCTACGQTGGGNCPDCG
                     AVMGDATYQETFDEEYQVEVQEDDPEEMEGAEHPHKENTGGNQHHNSDNETGETADHP
                     IKVNGHHEITSTSRTCDHLMIDLETMGKNPDAPIISIGAIFF"
     gene            complement(2094..2285)
                     /gene="ydfD"
                     /locus_tag="ACHKTW_004349"
     CDS             complement(2094..2285)
                     /gene="ydfD"
                     /locus_tag="ACHKTW_004349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein YdfD"
                     /protein_id="HFO6465263.1"
                     /translation="MNSAFALVLTVFLVSGVPVDIAVSVHRTMQECMTAATEQKIPGN
                     CYPVDKVIHQDNIEIPAGL"
     gene            complement(2282..2470)
                     /gene="dicB"
                     /locus_tag="ACHKTW_004350"
                     /gene_synonym="ftsT"
     CDS             complement(2282..2470)
                     /gene="dicB"
                     /locus_tag="ACHKTW_004350"
                     /gene_synonym="ftsT"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HFO6465264.1"
                     /translation="MKTLLPNVNTSEGCFEIGVTISNPVFTEDAINKRKQERELLNKI
                     CIVSMLARLRLMPKGCAQ"
     gene            2975..3295
                     /locus_tag="ACHKTW_004351"
     CDS             2975..3295
                     /locus_tag="ACHKTW_004351"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6465265.1"
                     /translation="MSQPPINPLKNMKIDYWYKALTVVGAALFVFNGTSFFDRYPVVP
                     LGFLSSGIFFIGLGEWINHPLKVRFIGPGVWTRGYNSSSCALGIIFDILGCFLIVTGV
                     VKFF"
     gene            complement(3431..3532)
                     /gene="ydfB"
                     /locus_tag="ACHKTW_004352"
                     /pseudo
     CDS             complement(3431..3532)
                     /gene="ydfB"
                     /locus_tag="ACHKTW_004352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344964.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfB"
     gene            complement(3534..3689)
                     /gene="ydfA"
                     /locus_tag="ACHKTW_004353"
     CDS             complement(3534..3689)
                     /gene="ydfA"
                     /locus_tag="ACHKTW_004353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFO6465266.1"
                     /translation="MDTIDLGNNESLVCGVFPNQDGTFTAMTYTKSKTFKTENGARRW
                     LERNSGE"
     gene            complement(3882..4289)
                     /locus_tag="ACHKTW_004354"
     CDS             complement(3882..4289)
                     /locus_tag="ACHKTW_004354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO6465267.1"
                     /translation="MNEKTLGQRIRERRKQVGLSQNGLSKAAGVSGSSISLWESDHTA
                     PRGQNLHRLAEVLQCSPTWILFGDEDKTPAPPVSLDSALDLSEDELEMLRLYRALPKS
                     EQQAQLSELRARVENFNRLFTELLEARKRNKHQ"
     gene            4367..4594
                     /locus_tag="ACHKTW_004355"
     CDS             4367..4594
                     /locus_tag="ACHKTW_004355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000476984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HFO6465268.1"
                     /translation="MFKSDVINFYGTKAKVAKAAGVDPSAVSQWQELVPEGRAMRLQE
                     ASGGELQYDPKVYDEYRKTKRAGRLNNENHS"
     gene            4578..5099
                     /locus_tag="ACHKTW_004356"
     CDS             4578..5099
                     /locus_tag="ACHKTW_004356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001578395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFO6465269.1"
                     /translation="MKITPEQAREALDAWICRPGMTQEQATILITEAFWALKERPNID
                     VQRVTDEGGAVDQRALGVNRVKIFERWKAIDTRDKREKFTALVPAIMEAIRINDFRLY
                     REISDGKSITYMIAGLNKEYGDVVESGLLFADPAVVDRETDELIEKAIAFKLAYRQQY
                     QQKAGWNYEPSFC"
     gene            5080..6045
                     /locus_tag="ACHKTW_004357"
     CDS             5080..6045
                     /locus_tag="ACHKTW_004357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6465270.1"
                     /translation="MSLLFAERPLVINTQLAMKIGLNEAIVLQQLHYWLRDTNSGMEC
                     DGVRWIYNTTEQWLEQFPFWSESTLKRAFASLKTLGLLRCEKLNKSKRDMTNFYTINY
                     GSELLDDGKLSESIGSKCAAPSGQNDTMEEVKMKRSIGSKRSNVIGSKWPDDLTENTT
                     EITTENKNTFRPEASQPDPQTTEQDFLTRNSDAVVFSAKKRQWGSREDLACAQWIWGR
                     IVGLYEQAASDDGEIMRPKEPNWTVWANDVRTMRMLDGRSHRQICEMFGRVQRDPFWV
                     KNIMSPSKLREKWDELVIRLGRSPVQRCVNHISEPDTEIPPGFRG"
     gene            6086..6508
                     /locus_tag="ACHKTW_004358"
     CDS             6086..6508
                     /locus_tag="ACHKTW_004358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFO6465271.1"
                     /translation="MAKVFTQEEREKIKGQVVELVRQSGRETLRQLEAKTGATRYLMS
                     VLAREQVASGDVYNSGYGLFPSEQARKDWQNARKKLSRAKVKKPAVVDPDLIWSLPDG
                     EIRRYDSRLNIICRECRKSEVMQRVLTFYQGNYREVML"
     gene            6505..6681
                     /locus_tag="ACHKTW_004359"
                     /pseudo
     CDS             6505..6681
                     /locus_tag="ACHKTW_004359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004203.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
     gene            6792..7457
                     /locus_tag="ACHKTW_004360"
     CDS             6792..7457
                     /locus_tag="ACHKTW_004360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HFO6465272.1"
                     /translation="MTVADFKRPKLELPNGAKKLLLHSCCAPCSGEVMEALQASGIDY
                     TIFFYNPNIHPQKEYLIRKDENIRFAKQHGVPFIDADYDTDNWFERAKGMEWEPERGI
                     RCTMCFDMRFERTALYAAENGFSVISSSLGISRWKNMQQVNDCGRRAVAHYPGMVYWD
                     YNWRKQGGSSRMIEISKREQFYQQEYCGCVYSLRDTNLHRKSQGRPLIKIGQLHYGKE
                     GQE"
     gene            8070..8225
                     /gene="hokD"
                     /locus_tag="ACHKTW_004361"
     CDS             8070..8225
                     /gene="hokD"
                     /locus_tag="ACHKTW_004361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFO6465273.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            8442..8693
                     /gene="rem"
                     /locus_tag="ACHKTW_004362"
     CDS             8442..8693
                     /gene="rem"
                     /locus_tag="ACHKTW_004362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein Rem"
                     /protein_id="HFO6465274.1"
                     /translation="MMNIEELRKIFCEDGLYAVCVENGNIVSHYRILCLRKNGAALIN
                     FVDARVTDGFILREGEFVTSLQALKEIGIKAGFSAFAEE"
     gene            8760..9038
                     /locus_tag="ACHKTW_004363"
     CDS             8760..9038
                     /locus_tag="ACHKTW_004363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6465275.1"
                     /translation="MARNVKYYNSDNSPVLVCTHERYSHAFKSEWFQHPPCTEEQAEW
                     IIQCYRRRGYEVKKALSLDYRHWIISVRLPYSERPPRASRTFQQRIWR"
     gene            9040..10089
                     /gene="ydfU"
                     /locus_tag="ACHKTW_004364"
     CDS             9040..10089
                     /gene="ydfU"
                     /locus_tag="ACHKTW_004364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFO6465276.1"
                     /translation="MRVLLRPVLVPELGLVVLKPGRESIQIFHNPRVLVEPEPKSMRN
                     LPSGVVPAVRQPLAEDKTLLPFFSNERVIRAAGGVGALSDWLLRHVTSCQWPNGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLDLLAQQNLTAWVIDVIRHAISGTQERE
                     LSLAELSWWAVRNQVADALPEAVLRRSLGLPAEKICSVYRESDIVPGEQTATSILKQR
                     TKNLAPLPYAHQQQKLPQEKTVVSITVDPESPESFMKLPKRRRWVKEKYTRWVKTQPC
                     ACCGMPADDPHHLIGHGQGGMGTKAHDLFVLPLCRKHHNELHRDTVAFEEKYGSQLEL
                     IFRFIDRALAIGVLA"
     gene            10103..10855
                     /gene="ydfT"
                     /locus_tag="ACHKTW_004365"
     CDS             10103..10855
                     /gene="ydfT"
                     /locus_tag="ACHKTW_004365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFO6465277.1"
                     /translation="MNLEALPKYYSPKSPKLSDDSPATGTGCLTITDVMAAQGMVQSK
                     APLGLALFLAKVGVQDPQFAIEGLLNYAMALDNPTLNKLSEEIRLQIIPYLVSFAFAD
                     YSRSAASKARCEHCSGTGFYNVLREVVKHYRRGESVIKEEWVKELCQHCHGKGEVSTA
                     CRGCKGKGIVLDEKRTRFHGVPVYKICGRCNGNRFSRLPTTLARRHVQKLVPDLTDYQ
                     WYKGYADVIDKLVTKCWQEEAYAEAQLRKVTR"
     gene            complement(11030..11232)
                     /gene="cspF"
                     /locus_tag="ACHKTW_004366"
                     /pseudo
     CDS             complement(11030..11232)
                     /gene="cspF"
                     /locus_tag="ACHKTW_004366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416076.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspF"
     gene            11531..11746
                     /gene="cspB"
                     /locus_tag="ACHKTW_004367"
     CDS             11531..11746
                     /gene="cspB"
                     /locus_tag="ACHKTW_004367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspB"
                     /protein_id="HFO6465278.1"
                     /translation="MSNKMTGLVKWFNADKGFGFISPVDGSKDVFVHFSAIQNDNYRT
                     LFEGQKVTFSIESGAKGPAAVNVIITD"
BASE COUNT         3105 a         2605 c         2974 g         3286 t
ORIGIN      
        1 tcgaaaaata ttgcacctat tgagatgatc ggggcatcag gattttttcc catggtttca
       61 aggtcgatca ttagatggtc acacgtcctg ctggtggatg tgatttcgtg atgaccgttc
      121 accttaattg ggtgatctgc cgtctcgcca gtttcattat cgctattgtg atgctgattg
      181 ccgccagtgt tctccttgtg tggatgttca gcgccttcca tttcctccgg atcatcttcc
      241 tgaacttcaa cctgatactc ttcatcgaat gtttcttggt atgttgcgtc gcccatcacc
      301 gcgccacaat cagggcagtt gccgccgccg gtctgaccgc aggcggtgca gaccttttcc
      361 acttcctgtt gcgccactgg ttcaggctgt ttcgtttctg gctcgttttg taacgcattt
      421 ggactgtttt gttccgcttt ttggtagttc cgttccgatt catgctggtt ctggttcaca
      481 gaatcgcggg tctggatccc cttaacccat ttcggatcat tcgggtcact aatcccttca
      541 acaaattcac cacgtgatgc agcaagcaac ttatcggcgt caggctggct gatattggct
      601 gcctgcataa ttttgtttac ttcgtcagcg gtaactttta tcggctctgg ttgttctgaa
      661 tcttcagcgg tatctacatt ttgcggtaag cccgtgtatg tgccattttt tcgggcaaaa
      721 tattcttctt ttgtgatttc agtggcgcca gcagccagtg ccttatccag accagaaagt
      781 ttgtttgcgc gaccgtattt ttctccgtcc ttatctgcga agaggaaata gaacggcccc
      841 tcacgctcta cagatggttc agcttccggc gcggtttcat tttttgggat atcagatacc
      901 tcagtttcca ctgcatcagt ttgtgtttct gatgactgga gaacatcaac agtgcccagg
      961 tctgtttctt cattctcaaa cacgcccttt gtcgtcaggt attcgcagat atatttgttc
     1021 agtgctacgg gatctttgtg aatgtcgatc ggacgctcac ggacaaggcc aaaaatagtt
     1081 tggcggtcgt agcgaagggc atcaggctgt ttgcgcattg atgccgagat acgcttccag
     1141 tcttcgcggt cgttgtcgat aacttctttt tttgcccagc gatggatgct gccgtcaatg
     1201 tttccggcat ccacatcacc aggccagaga gcgtaggcca gttcgtcatc cagtgttttc
     1261 catgtctgct tgtattcgcg atgaatggca gcaatgaccg ggctgatttt tcctgttgaa
     1321 ttttcagtgt actgttgatt ggctctggcg cgggcgagat caacaacaga cgtgtatttt
     1381 ccggtttcct tgcgttcacc ttcgcgacgt tttttccaga tgcgcatctc tgcctgaatt
     1441 tcgggccatt tagcaccagg aatacattta tgcttaaccc acccaatggc gtgcaactta
     1501 agctccggat acatggcgtt aacttctggc attttcatca acgcttcaac gatatgtccg
     1561 tcgaatgttg ccatgtcttc ctgcaacaat tcctgtgcgc taataaccat atcaacggtg
     1621 atgttttcac atgtgtcgaa cttaaccatg acagcgttct gtacttcagg ggccagcttg
     1681 tcaaaagtga cgttcatcgg atcggattca gtctcaaccg ggacaaaaga agcagactcc
     1741 tcatcccagc ggttttcctg catatattca gcatcccatg aatcgagggc agggcggggt
     1801 atgccaggtt tatcctcgca gacaataaat ttataagcgc agtcctgagc agccggataa
     1861 tgttccagga attgccagtg aaattttgct cgagcacggc gttcgtcgcc agcttcaatg
     1921 gctgtggcta cagccacagc gccttcttcc cttgttgcca gttcgtcagg aatagcggcg
     1981 caaataaaga ctttactcat tttgttttaa cctcatgaca gatttaagga tgaacaaatc
     2041 cctgccattg ctggcatata agaatgaaac cggatattta ttacggaact gttttaaaga
     2101 cctgccggga tttcgatatt atcctggtga ataactttat cgaccgggta acagttaccg
     2161 ggaattttct gttcggttgc tgcagtcata cactcctgca ttgtcctgtg aacactgact
     2221 gcaatatcaa ctggcactcc ggaaacaaga aaaactgtca gaacaagcgc aaatgctgaa
     2281 ttcattgtgc acatcctttt ggcatcagac gtaaacgagc cagcattgaa acaatgcata
     2341 ttttatttaa tagctcccgt tcttgttttc tcttgttaat ggcatcttca gtaaatacag
     2401 ggttactgat agtgacacca atttcaaaac aaccttcaga cgtattaacg tttggtaata
     2461 acgttttcat tatcgcgtcc tcaacaatga attttgtgat gcagtgcctg gtgcctccag
     2521 gtgacgttaa ccagttaaca attaacgtcg gatacagaga atccacccat aacactgttt
     2581 ttggttttaa ctgttccgcg tgcgcttagc cgcattcacc gcatcacaaa attcacttta
     2641 aaaacggcgg cagagcagtc acggagtaaa actgataccg ccaaacgtca ccagaaaatt
     2701 gataacagag ggcgttgcag cggggttgtc acttaagcgt atggtcaacc tgacaactcg
     2761 gtgtcctcaa cggggaagga ataaccccgc catacttacc gccgcgccat tttgcgggtt
     2821 gccacaaccg gaagcgcacg gtcgaattaa atttaacgac accgtacagc gagacgaact
     2881 tcgccgtgcg ctttcgtgtt gtgtgcctgc ttttaaccac gtcaggcgag gtggtatcct
     2941 taaaatcacc acagttttaa ggattcatta agcaatgtcg caaccaccaa taaatccgct
     3001 taagaacatg aaaattgatt actggtataa agcgcttaca gttgttggcg ctgcgttgtt
     3061 tgtctttaat ggaacgtctt tttttgacag atatcccgtt gttccattgg gttttttgtc
     3121 ctccggcatc ttttttattg gtttggggga gtggattaat caccctctca aagtgagatt
     3181 tattggtcct ggagtttgga ctcgtggata taatagttct tcgtgcgcac tcggtatcat
     3241 cttcgacata cttggttgtt tcctgattgt tacaggagtc gtcaagttct tctgatgtaa
     3301 aaccgcaaat ggggcacgta acgggaattt tgaaaagcgt ttctcagggt tccagaacaa
     3361 aattttctgc ggtctgattt tgcttctcat atttgtgctc cgcgtcattg tgagagcaca
     3421 ttcttattct gagtgcctgt ttaaactcac tgaagctgag agcttcttcg ccttcggcaa
     3481 ggccttcgaa gtattcttcg taagcctttt ccatgattgt gtcgaaatcc atatcactca
     3541 cctgagtttc tttccagcca gcgacgggca ccattttcgg ttttaaacgt tttgcttttg
     3601 gtatacgtca tcgcggtgaa cgtaccgtcc tggttgggga atacgccaca taccagagat
     3661 tcgttgttgc caagatcgat agtatccatg ctgacctcat ttccccttaa cgccggggta
     3721 gcggaactgt ttgctgagaa caccgtgcgg tgtcttgatg gaggtaattt agttttctca
     3781 ttgtttttcg tcaaggtttt ttgatgagaa aactcaagta ttggcgcaag ataaagccaa
     3841 tacattgaaa tgtaaggctt taaaattttg tgaagggatg attattgatg cttgttgcgt
     3901 ttgcgagcct ctaatagctc ggtgaacagg cgattaaaat tctcaacgcg ggcgcggagt
     3961 tcgctgagtt gtgcttgttg ctctgattta ggaagtgcgc gatacaaacg cagcatctct
     4021 aactcatctt ccgataagtc taaggcacta tcgagtgaaa cagggggagc tggtgttttg
     4081 tcctcgtcgc caaacagtat ccaggttggt gaacattgca atacctcggc gaggcgatgc
     4141 aaattttgcc cgcgcggggc tgtatggtca ctttcccata atgaaattga tgagccagat
     4201 acgccagcag ctttgcttaa accgttttga cttaagccta cctgcttacg tctttctcta
     4261 atgcgttgac ctaaagtttt ctcgttcata tttagatatc ttaataaccc ttgacttgag
     4321 attccttgag tgattagtat tgagaaaact caatattgga ggtgcgatgt ttaaatcaga
     4381 cgtaattaat ttttacggta cgaaagccaa agtagcgaaa gctgctggcg ttgacccatc
     4441 tgctgtttct caatggcaag agctggttcc tgaaggtcgc gcgatgcgtc tacaggaggc
     4501 atctggcggc gagcttcagt atgatcccaa ggtttatgac gaatatcgta agacgaagcg
     4561 ggcggggcgg ttgaacaatg aaaatcactc ctgaacaggc tcgtgaggct ctggatgcct
     4621 ggatatgtcg accaggaatg acacaggagc aggcgacgat attaatcact gaagcattct
     4681 gggctttgaa agagcgcccg aacatcgatg ttcagcgtgt cacagatgaa ggtggcgcgg
     4741 ttgatcagcg agcgcttggc gttaatcgag tgaagatatt cgaacgctgg aaggctatcg
     4801 acaccaggga taagcgtgaa aagttcacgg cgctagtgcc tgcaattatg gaggctatcc
     4861 ggattaatga tttcaggttg tatcgtgaaa ttagtgacgg aaaaagcatc acgtacatga
     4921 tcgccgggtt aaacaaagaa tatggcgatg tggtggagtc cggactgctt tttgctgatc
     4981 ctgccgtagt ggatcgtgaa actgacgaac ttatagaaaa agcaattgct ttcaaacttg
     5041 cgtatcgaca gcaataccaa caaaaagctg gatggaatta tgagccttct ttttgctgaa
     5101 cgcccactgg ttataaacac acagcttgcg atgaagattg gcttaaatga agccattgtg
     5161 ttgcagcagt tgcattactg gttgagagat accaattccg gtatggaatg tgatggtgtt
     5221 cgctggattt acaacacaac ggaacaatgg ctggaacagt tcccattctg gtcagagtca
     5281 acgttaaagc gcgcgtttgc aagtctgaaa acgctggggc ttttgcgttg tgaaaagctc
     5341 aataaatcaa agcgcgatat gaccaatttc tacacgatca actacgggag cgagctttta
     5401 gatgatggca aattgagcga atccatcggt tcaaaatgcg ccgctccatc aggtcaaaat
     5461 gacacgatgg aagaggtcaa aatgaaacgc tccattggtt caaaacgatc caatgtcatc
     5521 gggtcaaaat ggcctgatga tcttacagag aatacaacag agattactac agagaataaa
     5581 aacacttttc gtccggaagc ttcgcaaccg gacccgcaga cgactgaaca ggatttttta
     5641 acccggaact ccgacgcggt tgtgtttagt gcgaaaaaac gccagtgggg tagcagggag
     5701 gatttggcgt gtgcgcagtg gatctggggg cggatcgtgg gcctctacga acaggcagcc
     5761 agtgatgatg gcgagatcat gagaccaaaa gagcctaact ggactgtctg ggccaatgac
     5821 gtgcgcacaa tgcggatgct ggatggcaga agccacagac aaatttgcga aatgtttggt
     5881 cgggttcagc gagatccatt ctgggtaaaa aacatcatga gcccgtcaaa gctccgcgaa
     5941 aaatgggacg aactggtcat ccgcctggga cgttcacctg tacagcgttg tgttaatcat
     6001 atttctgaac cggatacaga aattccgcct ggtttcagag gataagtgtt gatttcaggt
     6061 catgaggtaa ttttaagggg gacttgtggc aaaagttttt acacaagaag agcgggaaaa
     6121 aattaaaggg caggtggtgg aactcgtgcg ccagagcggt cgtgagacgt tacggcaact
     6181 ggaagctaaa acaggtgcga ctagatatct gatgagcgtt cttgccagag agcaggtagc
     6241 cagtggcgat gtatacaact ccggctacgg gttattcccg tctgaacagg cgcgtaagga
     6301 ctggcaaaac gcccgcaaaa aactctcgag ggcaaaggtg aagaaacctg ctgtggttga
     6361 tccggacctt atctggtcat tacctgacgg agaaatacgc cgctacgaca gtcgtctaaa
     6421 cataatctgt cgcgagtgcc ggaagagcga agttatgcag cgtgttctga cgttttatca
     6481 ggggaattat cgggaggtga tgctgtgagt acatcggcta ccattctcga catgtgctgt
     6541 ggcagtcgca tgctctggtt cgataagaat gacgaatggt cgatatttag cgatatcagg
     6601 aaagaggaac attcgctatg tgatggacgt cacccgatta ttaatcccga cattatcgct
     6661 gattttcgtg cgctgccgtg gttgtctgct ttgcatcaaa agcagacatt accagattta
     6721 gacatctgtt tccgatagcc cgttctgatg ctacactttt tgctattttc atgaacctca
     6781 taaacacatt tatgactgtt gctgatttca aacgccccaa attggagctc ccaaacgggg
     6841 caaaaaaact attactgcac tcttgctgtg ctccatgttc cggtgaagtg atggaggcgc
     6901 ttcaggcctc gggaatcgac tacaccatct ttttctacaa cccgaacatt catcctcaga
     6961 aagagtattt aattcgtaag gatgagaata ttcgctttgc taaacaacac ggcgtgccat
     7021 ttattgatgc tgattacgac acagacaact ggtttgagcg agccaaagga atggaatggg
     7081 agcctgaacg agggatccgt tgtaccatgt gttttgacat gcgttttgag cggactgcac
     7141 tgtatgccgc tgaaaatggt ttcagtgtga tcagtagctc actgggcatt tcacgctgga
     7201 aaaatatgca gcaggttaac gactgtggac ggcgagccgt cgcgcattat ccgggcatgg
     7261 tgtactggga ttataactgg cgaaaacagg gcggctcttc ccgcatgatt gaaatcagca
     7321 agcgcgaaca attctatcag caggaatatt gtggctgcgt gtattcactg cgcgatacca
     7381 atctacaccg caaatctcag ggacgccctc taatcaaaat tggtcaactg cactacggta
     7441 aggaaggaca ggaatgattt tatattatgc caacggagcg tagcagtgtt tctatatata
     7501 gtacgcctac cttaattttc aaataatcat gattaatgag caggtcatca gtgcaagcaa
     7561 tgacctgctc atcttgttga aattagagga aatcttgcag ccatgatatg gcttaaaata
     7621 acgtttgatt attttacctg ctgtttgtcc ctggtttaaa gtcggttgtc tgatttgatt
     7681 acgcgcaact gacgcttacg tattgaagta acgagcacga agacacatat ttatcagtgc
     7741 ctgtatacgt caagagcagg agatttatgt gctggttctc aaatgcacgc tggctattgt
     7801 ggctgtaatg gcaatttatt gtcttgttat tgttcttatg gatcaccttt ctgattgatt
     7861 tcatattggc gaggtaacgg tagttaagta gaatggctgc gggtgcttga ggctatctgc
     7921 ctcgggcatg aacaccaacg gcagatagag aaaagcccca gttaacatta cgcgtcctgc
     7981 aagacgctta acattaatct gaggccaatt tcatgctaga cacatgtagg ttagcctctt
     8041 acgtgccgaa aggcaaggag aagcaggcta tgaagcagca aaaggcgatg ttaatcgccc
     8101 tgatcgtcat ctgtttaacc gtcatagtga cggcactggt aacgaggaaa gacctctgcg
     8161 aggtacgaat ccgaaccggc cagacggagg tcgctgtctt cacagcttac gaacctgagg
     8221 agtaagagac ccggcgaggg agaaatccct cgccacctct gatgtggcag gcatcctcaa
     8281 cgcacccgca cttaacccgc ttcggcgggt ttttgttttt attttcaacg cgtttgaagt
     8341 tctggacggt gccggaatag aatcaaaaat acttaagtag cgcgcaggga taagagggat
     8401 ggtcccttaa aggggagagc taattatccg gaaggattct gatgatgaac atcgaagaac
     8461 tgcgtaaaat tttttgtgaa gatggcctct atgctgtgtg cgttgaaaat ggaaacattg
     8521 ttagtcatta ccgcattctg tgtttgcgaa agaatggggc tgcgttaatt aattttgtgg
     8581 atgctcgggt cacggacgga tttatcttgc gcgaaggtga gtttgtcact tcattacagg
     8641 cactgaaaga gatcggaata aaagcaggct tttcagcttt tgcagaagaa taaactcatc
     8701 tacaatcttg cgcggggctg aactcccgct gagtaacacc gtgccaccgg agaaaaccga
     8761 tggcacgcaa cgtaaaatat tacaattctg ataattcgcc cgttcttgtc tgcacgcacg
     8821 agcggtattc tcacgcattc aagtctgaat ggttccaaca ccctccatgc actgaagagc
     8881 aggctgaatg gataattcag tgttaccgca ggcgcggata cgaggttaag aaagccctta
     8941 gcctcgacta ccgtcactgg ataatctcag tcagattgcc ttactccgaa cgcccaccac
     9001 gtgcgtcccg cactttccag caacggatct ggaggtaacg tgcgggtatt acttagacct
     9061 gttctggtgc ctgagcttgg gctggtggtc cttaagccgg gccgtgaatc catacagata
     9121 tttcataatc ctcgagtgct ggtggaaccg gaaccaaaaa gcatgcgtaa tctgccatcc
     9181 ggagtcgttc ctgccgttcg ccagccgctg gcggaagaca aaacattgct gccgtttttt
     9241 agtaacgaac gggtgattcg tgctgctggc ggcgttggcg cattgtctga ctggctatta
     9301 cgtcatgtta catcctgcca gtggcctaat ggcgattacc atcactctga aacagtcatt
     9361 caccgttatg gtaccggcgc aatggtgttg tgttggcact gcgacaacca actgcgtgac
     9421 cagacatcgg aatcactgga tctgcttgct caacaaaatc tgacagcatg ggtgattgac
     9481 gtcatccgtc acgcaataag cggtacgcag gaacgtgaat tatctctggc tgaattatcc
     9541 tggtgggcgg tccgcaatca ggtggcggac gcgctaccgg aagcggtatt acgtcgttcg
     9601 ctgggattac cagcggaaaa aatctgctcg gtgtaccgcg agagcgacat cgtaccggga
     9661 gagcagaccg ccaccagcat attgaaacaa cgcacaaaaa atcttgcacc gttgccttac
     9721 gcccaccagc aacaaaaact accacaggaa aagacggtgg taagcatcac cgttgatcca
     9781 gagtctccgg aatctttcat gaagctgcct aaacgtcgcc gctgggttaa ggagaaatac
     9841 acacgttggg ttaagacaca gccgtgtgct tgctgcggta tgccagccga cgatccgcat
     9901 catctgattg gtcacgggca gggcggaatg ggaacaaaag cacatgatct ctttgtgttg
     9961 cctttgtgca gaaagcatca caacgagctg catagggata cagtggcatt tgaagagaag
    10021 tatggctccc aactggagct gatatttcgt tttatcgatc gcgcgctggc aattggcgta
    10081 ctggcgtaag tggagaacga gcatgaacct tgaagcctta ccaaaatatt actccccaaa
    10141 atctccaaaa ttgagcgatg actctccagc gacaggcacc ggttgtttaa caattacgga
    10201 tgtaatggca gcgcagggga tggtgcagtc gaaagcacca cttgggttgg ccttatttct
    10261 ggcaaaagtt ggtgttcagg accctcagtt tgcgattgaa ggcctgctaa attacgcgat
    10321 ggcactggat aacccgacat tgaacaaatt gagtgaagaa atccggttac agattattcc
    10381 ttacctcgtg agttttgcct ttgctgatta ctccaggtct gcggcaagta aggctcgctg
    10441 tgagcattgt tcaggtacgg gattttataa tgtattgcgc gaagtggtga aacactacag
    10501 acgcggggaa tctgtaatca aggaagaatg ggtgaaggaa ctatgtcagc attgccatgg
    10561 taagggcgaa gtcagcacag cgtgcagagg gtgtaagggt aaagggattg ttctggatga
    10621 aaaaagaacc cggtttcatg gcgtaccggt atataagatt tgtgggcgtt gtaatggaaa
    10681 ccggtttagt cgtttaccga ccacgctggc acgacgtcat gtccagaagc tggtaccaga
    10741 cctgaccgat tatcagtggt ataaggggta tgcggacgtc attgataaac tggtaacaaa
    10801 gtgctggcag gaagaagcat acgcggaagc gcaattgagg aaggtgacga gataaatgat
    10861 ttttgctgaa gatggcgaca tgatgtttgc atttttcaaa aaacatggat aagattttct
    10921 caacgatggg ctttgtgtat ccgacgttta gaaaaaagta gaaaacccgc tgataagcgg
    10981 gtttttgtgc tttaaatggg gcaatagaga tattgaatct catcccggga taaacattgg
    11041 gagttgaagg tccacgcgaa ccatttatcc ggcaaaattc cacgcgtaat cctgtggtaa
    11101 tttcttctgc atctcgaaga ttgagagctg aaacgtgaag ctgggcatcg atacgccatc
    11161 ggatgggaat ataagacctt tgctgctttt gtagtcaaag tttttgacaa ttcctgtcat
    11221 tttaggggac agaaaaactc cttaatactg ataacctggt gcaccataca cacgttcctg
    11281 gagaaaacta ctttgttgat agggttgaag gtgtctggat gtctaaaata aacattgctt
    11341 catatgttca actatgagtt aatgactgcg tcggtttgaa gaacagacga tatacgaagt
    11401 agtttactaa agcagttctc atttcaggtg ttattcactt attccttctt tgagtctctc
    11461 caattaagta cgaagtcgtt tctgttatgc aaaccattta tgccgaaagg ctcaagttaa
    11521 ggaatgtaga atgtcaaata aaatgactgg tttagtaaaa tggtttaacg ctgataaagg
    11581 tttcggcttt atttctcctg ttgatggtag taaagatgtg tttgtgcatt tttctgcgat
    11641 tcagaatgat aattatcgaa ccttatttga aggtcaaaag gttaccttct ctatagagag
    11701 tggtgctaaa ggtcctgcag cagtaaatgt catcattact gattaaaatt catcgctcgt
    11761 ctgtatacga taacgaagaa ggctgatgcc tgagtagaga tacggacaga gtagtgaata
    11821 ttggatttct ttaataaaaa ttaaggaggt ccaatacatg aaacaatggc cagcatattt
    11881 ggcaaaatct taatcaggaa aagtatgcta accattgtgg tgaagtgcag gtttgctgca
    11941 tgaatagttt tacagcagaa gctaactgct
//
LOCUS       DAZFQD010000156        11787 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.156, whole genome shotgun sequence.
ACCESSION   DAZFQD010000156 DAZFQD010000000
VERSION     DAZFQD010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11787
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.156"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            95..451
                     /gene="yffB"
                     /locus_tag="ACHKTW_004368"
     CDS             95..451
                     /gene="yffB"
                     /locus_tag="ACHKTW_004368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HFO6465279.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPAIIKRPLLCAPGKPM
                     LLGFSDSSYQQFFHEV"
     gene            455..1582
                     /gene="dapE"
                     /locus_tag="ACHKTW_004369"
     CDS             455..1582
                     /gene="dapE"
                     /locus_tag="ACHKTW_004369"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HFO6465280.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            1610..1810
                     /gene="ypfN"
                     /locus_tag="ACHKTW_004370"
     CDS             1610..1810
                     /gene="ypfN"
                     /locus_tag="ACHKTW_004370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HFO6465281.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(1920..2054)
                     /locus_tag="ACHKTW_004371"
                     /pseudo
     CDS             complement(1920..2054)
                     /locus_tag="ACHKTW_004371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416968.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
     gene            complement(3415..3555)
                     /locus_tag="ACHKTW_004372"
     CDS             complement(3415..3555)
                     /locus_tag="ACHKTW_004372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO6465282.1"
                     /translation="MPQKTIIISILCLTMLLTVWMIHRSPCEFRLKIMWSEFAAFLQC
                     KP"
     gene            4229..4657
                     /locus_tag="ACHKTW_004373"
     CDS             4229..4657
                     /locus_tag="ACHKTW_004373"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO6465283.1"
                     /translation="MTKPLNAFMSAWGKSPADATKKKGTTTHLNAHRETTQIATVSYV
                     LLTACRHLKVSRRQFAKGSGLSHTTVNRLLRGEVKITPEIALKLEYYTGVDAAFCLAL
                     QLQEMRETTDYGTAIQSSLDWRESVERLVTVSQHNQQGRQ"
     gene            complement(4730..6076)
                     /locus_tag="ACHKTW_004374"
     CDS             complement(4730..6076)
                     /locus_tag="ACHKTW_004374"
                     /inference="COORDINATES: protein motif:HMM:NF016981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P2 family phage major capsid protein"
                     /protein_id="HFO6465284.1"
                     /translation="MSGISKRFVGGVKKLMGVESSRADKFQEPEVKNTDAHLHEHGDH
                     ERDTTITHANSRSKTAMTLTRLSGDAPEQKNNAGDCGDSYAFCGAAAARDSVTAEKNR
                     HAQRVLNALAEGFAMKPIDSIRMSGDQSETIRVQAEGVEPVPVRSLKLVESWSTNFLE
                     RINILRLEDSKIEVCLINTGGTIASTGGNRHKRNIDAGKNTPRVYDLLQVNFDSQLSY
                     DTLSGASGASDDVLVSRYLEAIERRKMLDVIMTGFSGTHHALMSNPATYPNREDCGRG
                     WLQAIREEAAHRVYSGMSVSSFDQTGQIISVGDYASVDALVMDALNTAIDEHFRRDLV
                     AVCSSKLLNVKNHRLLNNLSGSNAPNSEILMHDEIRANPSLGGVPAVCVPFFPDDAVL
                     VTPLANLSWYWQKGSWRKHFKNEPEFNRMAFYESVKACYVVEEYDRTCLIEAIKLL"
     gene            complement(6073..6792)
                     /locus_tag="ACHKTW_004375"
     CDS             complement(6073..6792)
                     /locus_tag="ACHKTW_004375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6465285.1"
                     /translation="MSEQLKQLSEQYQAASIILHDAINTYRKKTELVDNLAESEKVLR
                     ARIDAHSAEWAEKLDAENGRITGDVDKVALLTAASSAELPVVIQRQQSAGLEKLEALA
                     DLVSKGKRFIAARRELCKGAAEEEIQRLTEEAISAVRLVFAFNQVCGGVKGFHKLQGV
                     ITDACDCGHESLDAARQHLEKQIKDGNNTLAQLPREAVPSILSKTRKYPSAAQMYRAK
                     RDPDYALQLISGAVAMTGGES"
     gene            6966..7163
                     /locus_tag="ACHKTW_004376"
     CDS             6966..7163
                     /locus_tag="ACHKTW_004376"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6465286.1"
                     /translation="MSKNQKITAKTRYRGIRIPGELDRLIEAESRKQKTTYSKIVIRL
                     LRYQLIPGGQQYRVIDEILDD"
     gene            7253..7522
                     /locus_tag="ACHKTW_004377"
     CDS             7253..7522
                     /locus_tag="ACHKTW_004377"
                     /inference="COORDINATES: protein motif:HMM:NF016487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ogr/Delta-like zinc finger family protein"
                     /protein_id="HFO6465287.1"
                     /translation="MAKPKQNHRFFPCPWCGSDTFCRTSRAASRECTVKENHRRCTNP
                     ECNARFVTFEHIHRIISIPVTPNGGQGVSLHEASAIKEKIGSLGL"
     gene            complement(8252..9229)
                     /locus_tag="ACHKTW_004378"
     CDS             complement(8252..9229)
                     /locus_tag="ACHKTW_004378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011815648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO6465288.1"
                     /translation="MSIREMHDGRYLVDVRPQGAEGKRIRKIFTLKSKAQEFERYVLQ
                     NFHDKPWQAKPADQRRLSELIDAWWVLDGRNQPYGDTYRVRLEKINREMRAPRASEMT
                     RKFMLGYRSDKLQAGLMPSSVNRDLCALSAMFTSLIDAEVFHNENPVRGIRKLKVRNT
                     EMAFLSDDEIDRLLERLEGDARRVAVLCLSTGARWREAAGLRGEHIVGNRVMFFNTKN
                     GKSRAVPVSDTVLSLIKTRKTGLLYQVDYIRFRDILQEVKPDLPKGQATHVMRHTFAT
                     HFMMNGGNIIVLQRILGHATIQQTMTYAHFAPDYLEESTRLNPLKGIAI"
     gene            complement(9622..11757)
                     /locus_tag="ACHKTW_004379"
     CDS             complement(9622..11757)
                     /locus_tag="ACHKTW_004379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HFO6465289.1"
                     /translation="MKAPNLKLQQSDKMTEVIIFAGSDAWSHAKEWKEWAGKGIAGDN
                     TPPVILGPQQLQNLNNIEIIDKGRHYARVYRAGEISEKHLLHIATLISLAGVKELRIY
                     RSFDDREPENLTARLPALKQDAESGKSIVMQGKAQNLQTSRQEELKPRIEVREEGVYW
                     VTPKLERSGEIIRPEEWLCNPVEMIGEGVINKEHYRVMRWKSDVTGETLTAAIPCGDI
                     GDNEGWKALKRGGLKVTANKSHRSILANWMQLDGCHDEWHLSNTSGWHFGRYIMPDGQ
                     AIGESDKPILFTGKTAAVNGYSVKGTAESWRDSVARLANGNPSMMISVAASLAAPIIG
                     IIGADSFGLHLFESSTAGKTTAQSVASSIWGNPERLRLSWYGTALGMANEAEAHHDGL
                     LPLDEIGQAASARDVYESAYTIFNGLGKLQGAKDGGNRELRRWRTVAISTGEVDIETY
                     LKKEGVTVKAGQLIRLLNVPMQKAQELHEFSNGKDHADALKENWSENHGAAGREWIKW
                     LAEHQQEVKDAAKESRNRWRKLVPEDYGEQSHRAAERFAIMETALELSTWLTGWNAAQ
                     CREATEHNFSLWVDGFGKGNKDHQQILYQATEFLNRYGESNFLPLPGDPERAKIQHLY
                     GYRTPFYEKEKRGTDYYVFRSVFEEEIAKGFNKNTAAAVLYHLGWLKKSKSEEGWTSR
                     TPRLEHKGGKRERVYHIHIDFSAEDEDQE"
BASE COUNT         3027 a         3074 c         2627 g         3059 t
ORIGIN      
        1 tcgataatgt tatatttcct gataatcatt tgcaggcaaa atgttttcac ccttaaatga
       61 gtatttattc tcatagatcg aaaaaggatt cattatggtt acactttacg gcatcaaaaa
      121 ttgtgacacc attaaaaagg ctcgccgttg gctggaagcc aataacatcg actatcgttt
      181 tcatgattac cgcgtcgatg ggctggacag cgaattattg aacggtttta tcaacgaatt
      241 aggctgggaa gcgttactga acacccgtgg tacaacctgg cgtaaactgg acgaaaccac
      301 ccgcaataaa atcaccgatg cggcctctgc ggcggcatta atgactgaaa tgcctgcaat
      361 tatcaaacgt ccattgctct gcgcgcctgg taagcctatg ctgctgggtt tcagtgattc
      421 cagttatcag caatttttcc atgaggtgta gtctatgtcg tgcccggtta ttgagctgac
      481 acaacagctt attcgccgcc cttccctgag tcctgatgac gcaggatgcc aggctttgtt
      541 gattgaacgt ttgcaggcga tcggttttac cgttgaacgc atggactttg ccgatacgca
      601 gaatttttgg gcatggcgtg ggcaaggtga aacgttagcc tttgccgggc ataccgacgt
      661 ggtgccgcct ggcgacgccg atcgttggat caatccgccg tttgaaccca ccattcgtga
      721 tggcatgtta ttcgggcgcg gtgcggcaga tatgaaaggc tcgctggcgg cgatggtggt
      781 agcggcagaa cgctttgtcg cacaacatcc caaccatacg gggcgactgg catttctgat
      841 cacctctgat gaagaagcca gtgcccacaa cggtacggta aaagtcgtcg aagcgttgat
      901 ggcacgtaat gagcgtctcg attactgcct ggtcggcgaa ccgtcgagta tcgaagtggt
      961 tggtgatgtg gtgaaaaatg gtcgtcgcgg atcgttaacc tgcaacctta ccattcatgg
     1021 cgttcagggg catgtggcct acccgcatct ggctgacaat ccagtacatc gcgcagcacc
     1081 tttccttaat gaattagtgg ctattgagtg ggatcagggc aatgaatttt tcccggcgac
     1141 cagtatgcag attgctaata ttcaggcggg aacgggcagt aacaatgtta ttccgggtga
     1201 actgtttgtg cagtttaact tccgcttcag caccgaactg actgatgaga tgatcaaagc
     1261 gcaggtgctt gccctgcttg aaaaacatca actgcgctat acggtggatt ggtggctttc
     1321 cgggcagcca tttttgaccg cgcgcggtaa actggtggat gcggtcgtta acgcggttga
     1381 gcactataat gaaattaaac cgcagctact gaccacaggc ggaacgtccg acgggcgctt
     1441 tattgctcgc atgggggcgc aggtggtgga actcgggccg gtcaatgcca ctattcataa
     1501 aattaatgaa tgtgtgaacg ctgccgacct gcagctactt gcccgtatgt atcaacgtat
     1561 catggaacag ctcgtcgcct gatgagtggt tctgcaagag gaaataagca tggactggct
     1621 ggctaaatat tggtggattc tggtgattgt ctttttggta ggcgtcctgc tgaacgtgat
     1681 taaagatctc aagcgcgtcg accataagaa atttctcgcc aacaagccgg agcttccccc
     1741 gcatcgtgat ttcaacgata agtgggacga tgacgacgac tggccgaaaa aggatcaacc
     1801 gaagaagtaa gctgagtgct caaccgtagg ccggataagg cgttaacgcc gcatccggca
     1861 atggtgaacg atgcctgatg cgacgctcac gcgtcttatc aggccaggta atctgtcttt
     1921 tacatcattt caatcacatc atcatcgcca ggcttaccgc cacttagcgc ttcgtcgaaa
     1981 taatgtttcg gaatggtata acgcaagtga tccagggcaa attgcatact gcgattgtcg
     2041 atagcatgac ataaaggcta attaattatt aaaaatcatt aagttatatt tttgaagatc
     2101 atcaaaacac aaaaggatag gtgaaaggat agatttttaa cacccataac catagaacct
     2161 ttcaagaatg cttaaatcat ggggcataga taggcctgta gcgaaaaaat tagagcatag
     2221 taatggggta tcaccacaaa cagtaaaaaa cagcgtcaga atttaattta agtgggttat
     2281 tggtggtgtt agatctgggt gtgaattact tcaggttgac tttgcagaat gtaacataat
     2341 attacacgaa aaaaaatgtt tcatgattgt taaaataaca tcatctgcgc cagatggatt
     2401 tttttagcat catcaccccc agaaagtacc cccaagttat acacacaaaa aacagcgatt
     2461 aatatctaac caaaatatta tgttaaatac gttttctcta taggttactc aaaaacacct
     2521 gtcccacctg tcccaaaaag gttatgcatt tgatttaatt ggaaatgttt cgaaatttgg
     2581 gacagccacc cccccgtttg ggacagcgca accccctctt gggacaactt tttgactttt
     2641 ttgggacagc tgggacagcc ggaaattttt aatcagggaa aataagaaca atttatagac
     2701 aatatcaggc taataaccaa ccacaaaaag acaaaaaaaa tcccatttag cagactaaaa
     2761 aacagccaac aagaaaaatc cgactcattt tttttgctaa accccatcga gttcgattcc
     2821 cttataatta ctacaaggaa taaacaaggc aatttttaat ttcgttttta aatatgcaga
     2881 ctctgcataa cactgataaa tgtaataaaa atgtaaaaca cactttgcgc caccggcgaa
     2941 aaaatgcgct taattgagcg aactactggg ggtcagtttg gagatcggaa atttttgtac
     3001 gttaagtgca ttatttaacc aatcccgatg tgtaacggat tgattttacg tcagcagtta
     3061 tcgcttttaa gttgtcgctc ttgcggtatc gcatttaggt tatccgatta atgccaactc
     3121 ccctgaaaac accgtatagc gcgggagtac accctatcgc gataggtaag cctgttcaaa
     3181 aaacaggctt accgtacaat aattctctat atccaaacta acccctactg acattgcgag
     3241 gatgtttttg gtgtgttcag aatgctgatc gcaaccagac acccacgagg cgggacaaaa
     3301 aacccgcaca atttacgcgc gggctttgct gtttgtgtgt gtatttgata gggcgccttt
     3361 acggccttac cctggcaacc ggatagcggg gactttcgcc cccgctgcgg tttcttacgg
     3421 tttacactgc aagaacgccg caaactctga ccacatgatc ttaagcctga actcacacgg
     3481 cgaacggtgt atcatccaca cagtcagcag catggtcaga cataaaatac ttatgatgat
     3541 cgttttttgc ggcataagcg cttgctcctt ttacagagag gcgctaacct tccacttgat
     3601 tagggtagga ctgttagggc ctcgggttaa cgttagttga ctcggggcct tttcacatcc
     3661 ggccttcagg ttcaccttcc agccatcagc cgaaaggcgc ccgcgcgtaa tttacccctt
     3721 tttcatccct gcgacaattt gcggcattgc tgcatagctc tgtcagtcag ccataaacgg
     3781 atcagcctgg tcggttcgtg tgggggttgt tttttccggt ttatacagtc agaacgccgc
     3841 cagaagggac gggttatcag ggcttattag tgggttgctt gagaggttgt tttttcgtca
     3901 tggggacagt tattgccacg aatccaagat gcgcaaaagc gcactaaaac ccagaaacca
     3961 gaacaaggtg aaaagagagc aaacacgcat tattatattg attactaatg tttttcattc
     4021 tgttgctgcc ttatattttc cttccatttt cacttccaga catgagcaaa aatcagccaa
     4081 caattaaccc ggcgcgccaa aattattcca cctgacgacc tgtttttgtg gtggctggaa
     4141 caaatttgtt ccgcctgtaa aatgagcgtt tgccgccagt tgcgcggaca tgtgaacata
     4201 gtcctacaga ccgttaagga tcgcaacaat gacaaaacca ttaaacgcct ttatgagcgc
     4261 gtggggtaaa agtccggcag atgccaccaa aaagaaaggg acaaccaccc acctgaacgc
     4321 gcacagggaa accacgcaaa tagcaaccgt gtcttatgta cttttaacgg cctgccgcca
     4381 cctgaaagta agccgcagac agttcgccaa aggttcgggg ctatctcaca cgactgtaaa
     4441 caggttgcta cggggagagg taaagattac gccagagata gcgctaaaac ttgaatatta
     4501 cacgggggtt gatgcggcct tctgtctggc cctccagtta caggagatgc gcgaaaccac
     4561 agattacggc acagccatac aatcatctct ggactggagg gagtcagtag agagattggt
     4621 cactgtttca cagcacaacc agcaaggcag acaataaaaa aaccgtggca acacatgcca
     4681 cggtacgcga aataatgcgt aaggggtcgc acccccgaaa ggagtttttt tacaggagtt
     4741 ttatagcttc aatcagacag gttcgatcat attcttcaac cacatagcac gcttttacag
     4801 attcgtagaa cgccatgcgg ttaaattccg gttcgttttt aaagtgcttg cgccagcttc
     4861 ctttctgcca gtaccaggac agattagcca gcggggtaac aagcacagca tcatcaggga
     4921 agaacggcac gcacaccgca ggaacgcccc caagactcgg gttagccctg atctcgtcat
     4981 gcatgagaat ctcactatta ggcgcattcg accccgaaag gttgttaagt agtcggtgat
     5041 ttttcacgtt aagcagctta ctgctacaaa cggcaaccaa atcgcggcgg aaatgctcat
     5101 ctatggcggt gttaagcgca tccattacaa gcgcgtcaac gctggcataa tcccccacac
     5161 tgataatctg accagtctga tcaaaagatg acacagacat cccactataa acacggtgcg
     5221 ccgcttcttc acgaattgcc tgcaaccagc cgcgcccgca atcctcgcgg ttcggatatg
     5281 tagcggggtt ggacataagc gcgtgatgtg tacctgaaaa gccagtcata atcacatcaa
     5341 gcatcttgcg ccgctcgatt gcttccagat acctggatac aagaacatca tctgaagccc
     5401 ctgaagctcc tgatagcgtg tcgtaactaa gttggctatc aaagttcact tgaaggagat
     5461 cataaacgcg cggcgtgttt ttccccgcgt caatgttacg cttgtgacga ttgccgcccg
     5521 ttgatgcaat ggtcccgcca gtattaatca aacagacttc aattttgcta tcttccagcc
     5581 gaagaatatt gattctctca agaaagttgg tgctccagct ttctaccagc ttcagcgatc
     5641 gcactggcac aggttcgacc ccttccgcct gcacacggat tgtttcagac tgatcgccgg
     5701 acatcctgat cgaatcaatc ggcttcattg cgaaaccttc cgccagtgca ttaagcacgc
     5761 gttgcgcgtg gcggtttttt tctgccgtca cagagtcacg cgcggcagct gctccgcaaa
     5821 aagcataact atctccgcaa tcaccagcgt tgttcttctg ctccggtgca tcccccgaca
     5881 acctagtaag cgtcatcgcc gttttgctgc gactgttagc gtgtgtgatt gtagtgtcgc
     5941 gctcatgatc accatgctca tgaaggtgcg catccgtgtt ttttacttcc ggttcctgga
     6001 acttatcagc gcgtgacgac tcaacaccca tcagtttttt tacaccacct acaaagcgct
     6061 tgctaatgcc gctcatgatt cgccccccgt catcgccaca gcgccagaaa tgagttgcaa
     6121 ggcgtagtca ggatctcgtt ttgcccgata catctgcgcc gcagaaggat acttgcgcgt
     6181 ttttgagaga atcgacggaa ccgcttcgcg cggcaactgt gcaagcgtgt tatttccgtc
     6241 cttaatctgt ttttccaggt gctggcgtgc tgcgtcaagt gattcatgac cacaatcgca
     6301 cgcatcagta attaccccct gtagtttatg gaaaccctta acccctccgc aaacctggtt
     6361 aaacgcaaaa acaagacgca cggcgctgat cgcttcttcg gtcagtcgct gaatctcttc
     6421 ctctgccgcc cccttgcaaa gctcgcgacg cgcagcaata aaccgctttc ccttgctcac
     6481 aagatcggca agtgcctcga gtttttccag tccggcagat tgctggcgct ggatcactac
     6541 gggcagctct gccgatgacg ctgcggtaag tagcgccacc ttgtcgacat cgccagtaat
     6601 gcgcccgttt tccgcgtcca gtttttcggc ccattcagcg gaatgcgcat caattcgcgc
     6661 cctcagcacc ttttcagact ccgcaagatt atcgacaagc tcggtttttt ttctgtaagt
     6721 gttgatagca tcatgaagga taatgcttgc tgcctggtat tgctctgaca gctgtttaag
     6781 ttgttcggac attgttatca ctcctgttcg ttttatcccc ctcatactcc gcgaccgcca
     6841 aacacttttc tactcacgcc cgttgcaagg cgtgttttac aacaaacctg aaaagccaga
     6901 cccacagagc gcgggcatac tcatctcatc ccatcccgcc cacaggaaca acggagataa
     6961 acacgatgag caaaaaccag aaaatcacag caaaaacacg ctatcgcgga atacgtatac
     7021 ctggagaact tgatcgcctg attgaagccg aaagcagaaa gcagaaaacg acctacagca
     7081 agatagtcat ccgcctgctt cgctaccagt tgataccagg cgggcagcaa tacagggtta
     7141 ttgatgaaat cctcgacgac tgacgggcag aatggcaacc actaaacgac cgcttacggt
     7201 gtaaaatccc catgaagcgc actatctgcg cccatcatga aaggggttgt taatggcaaa
     7261 accaaaacag aatcacaggt tcttcccgtg cccgtggtgc ggttccgaca cattttgcag
     7321 aacatcaagg gccgcaagcc gtgaatgcac cgtaaaggaa aaccacaggc gttgcactaa
     7381 cccagaatgt aacgccagat tcgtgacctt tgagcacatt caccggatca tctccatacc
     7441 tgtaacaccg aatggcgggc aaggcgtgtc actgcatgaa gccagcgcca tcaaggagaa
     7501 gataggatcg ctcgggcttt aaccctctac accgccgcaa cacggacgcg gcgcacctcc
     7561 acagcacgcc atttaataat gatttgttaa acggtataaa atcagtgtta tcacctacag
     7621 aacaaacaca cccgcaacaa tcataaatct cacttttccg gcgctcattc ccacacctca
     7681 cagaacgcaa tcagacgcat cataacggcc tgtttctctg accatgttcc tgatgtgttt
     7741 ttttaaaact ggcatcactg cgtgcgtata acgcgcaaca cgaccccaaa taaagcaacc
     7801 tgccttctat cccccaattc gtgcaccctt ccctcgtcgc ctgcacgcag cgatcgcttg
     7861 ttttttcgtg caccttgcaa actggcctca gccagcgccg ttactgggct gaaagggtaa
     7921 aaaatacatt agaaaaattg tgcaaattcg tgcacttttg tgcacctgca atggtgagaa
     7981 tgagtaaaaa acaaccaaca taaaaagaga tgttttttat gcctgtttaa catcaaaaat
     8041 agttttatta ttcatgcgct tacagttatt tttttccgtt tcataagcaa catagcccac
     8101 gcacgcgcgc gtaattattg aagtgcgcaa aaatggctgt aaaacaccgt ttgcggaatg
     8161 cggttaacaa gggtaattta gcctccgaac ggaggcagca agacgaaaaa aaacacgcca
     8221 ccgaaccaca tcggcagcgc gttgagtgtg gtcaaattgc gatgccttta agcgggttca
     8281 gcctggtaga ctcttccaga tagtcggggg caaaatgtgc gtaggtcatg gtttgctgta
     8341 ttgttgcgtg ccctaatatc cgttgcagca caataatatt gcctccgttc atcatgaaat
     8401 gcgtggcgaa cgtgtgacgc ataacgtgag tcgcctgacc tttgggtaaa tccggtttca
     8461 cctcctgaag gatatcccga aaacggatgt aatcaacctg gtacaatagc ccagtttttc
     8521 gggtttttat caacgaaagc accgtatcag aaacaggaac tgcgcgggat tttccattct
     8581 ttgtgttaaa gaacattacg cggttaccaa cgatatgttc accacgcaaa ccagccgctt
     8641 ccctccacct tgcgccagtg gaaagacaca gaacagcaac gcgacgcgca tccccctcga
     8701 gtcgttcaag cagccgatca atctcatcat ctgaaagaaa ggccatttct gtattcctga
     8761 ccttgagctt acgtattcca cgcaccgggt tttcgttatg gaaaacctcc gcatcaatca
     8821 gcgaggtaaa cattgcagac aaggcgcaca aatcacgatt aacactggag ggcattaatc
     8881 cggcctgtaa tttatctgaa cgatacccga gcataaactt tcgcgtcatc tcactggcgc
     8941 ggggggccct catctcacgg tttatttttt ccagcctgac cctgtaagta tccccgtaag
     9001 gctgattccg accatcaagc acccaccagg catctatcag ttcggataac cgacgctgat
     9061 cagcaggctt ggcctgccac ggtttatcat ggaaattctg caaaacatac ctttcaaact
     9121 cctgtgcctt tgacttcagg gtaaaaatct tacggatccg ctttccttcc gccccctgcg
     9181 gtctgacatc aaccagatag cgcccgtcat gcatctctct gatgctcata actattctcc
     9241 ctcactttct gtaagtgtgt gcgcacctcc taaaaagagc gcttttgaag tgtttttttg
     9301 gctgttcagt ggttcgctcg ctgttcaatc aatagcagaa taaaacacta cccaagccgc
     9361 tttaactttc gaatgaaagt taaaacaaag aataacgtaa gcacatcaaa accaccttcc
     9421 caaacacgag ttgtcccaaa cgggggtttt tctgtcccaa gcgggggtaa atttgtccca
     9481 tgcaggggtt tagttgtccc aaaggggggt tgagttgtcc cagagaataa atacctttcc
     9541 ttataaatca tatacttaac tcattttata tggcttggga caactttttt ccccttgcaa
     9601 tggttctata ggggcttttt actactcctg atcctcatcc tcagcactga aatcaatatg
     9661 gatgtgatat acccgctccc ttttaccgcc cttgtgctca agcctcggcg ttctcgacgt
     9721 ccatccttct tctgatttgc tctttttcaa ccatcccaga tggtaaagaa ccgccgccgc
     9781 cgtgttcttg ttaaatcctt tggcgatttc ttcctcaaaa accgaacgaa aaacataata
     9841 atcagtgccg cgtttctcct tctcgtaaaa cggcgtcctg tatccgtaca gatgttgaat
     9901 cttcgccctc tcaggatcgc cgggcaaagg caagaaattt gactcgccat aacggttaag
     9961 aaattccgtt gcctggtaaa ggatttgctg atggtctttg tttcccttgc caaagccatc
    10021 gacccacaaa gaaaaattat gctcggttgc ttccctgcat tgcgcggcgt tccatccagt
    10081 taaccaggtg gacaactcaa gcgctgtctc cataatggcg aatcgttcgg cggccctgtg
    10141 ggactgttcg ccatagtctt cagggaccag ctttcgccac ctgttgcggc tttcctttgc
    10201 tgcgtccttt acttcctgct gatgctctgc cagccacttg atccactcac gccccgccgc
    10261 gccgtggttt tctgaccagt tttcttttag tgcatctgca tgatccttac cgttagaaaa
    10321 ctcgtgcaac tcctgcgcct tctgcatagg aacgttaagc agcctgatca gttgccccgc
    10381 cttaactgtt accccttctt tcttgaggta tgtttcaatg tccacctctc cggtgctgat
    10441 tgccacagtt cgccagcgcc tcagctccct gttaccgcca tcctttgccc cctgaagttt
    10501 tcccagtccg ttaaagattg tgtatgcgga ttcgtaaacg tcacgggcgc ttgctgcctg
    10561 gccgatttca tccaacggta ataagccatc gtgatgcgct tctgcttcgt tagccatacc
    10621 cagcgccgtt ccgtaccatg acaaacgcaa cctttcagga ttaccccaga tacttgaggc
    10681 cacgctttgc gcggttgtct tccctgccgt tgatgactca aacaaatgca atccgaaact
    10741 atcggcaccg ataatgccga taatcggcgc ggcaagtgat gcagccacag aaatcatcat
    10801 tgagggatta ccattagcca gccgcgccac gctgtcgcgc cagctttccg ccgtcccctt
    10861 cactgaatag ccattcacgg cggcagtttt tccggtgaac agtattggtt tgtctgattc
    10921 gccgatcgcc tgcccgtcgg gcatgatgta gcgtccaaag tgccatccag atgtgttgct
    10981 taagtgccat tcatcatgac acccatcaag ctgcatccag ttagcaagta ttgacctgtg
    11041 cgatttatta gccgtgacct taagacctcc ccgcttaagt gctttccagc cttcgttatc
    11101 gccaatatca ccgcacggga tcgccgctgt taacgtctct cctgttacgt cgcttttcca
    11161 gcgcataacc ctgtaatgct ctttgttaat taccccctct ccgatcatct ccacggggtt
    11221 acataaccat tcttcaggcc tgatgatttc gcctgacctt tcaagttttg gcgtgaccca
    11281 gtaaaccccc tcttccctta cctcaattcg tggttttagc tcctcctgtc tgcttgtctg
    11341 taggttctgc gcttttccct gcataacgat acttttcccg ctctcggcat cctgttttag
    11401 cgccggaagt cgagcggtaa gattttccgg ctctcggtca tcgaaactgc gataaatacg
    11461 cagttcttta acgccagcca gggaaatcaa ggttgcaata tgcaaaaggt gtttttctga
    11521 tatctctcct gcacgataaa cacgagcata gtgacggccc ttatcaataa tctcaatatt
    11581 attaagattc tgaagttgtt gagggccaag aattacggga ggtgtattat ctcccgcgat
    11641 cccctttcct gcccattcct tccactcttt cgcgtgactc catgcgtcac tacccgcaaa
    11701 gataattacc tccgtcattt tgtcggattg ctgtaatttt aaattgggtg ctttcattgc
    11761 ccccccctta tgttttcaga tgtaaag
//
LOCUS       DAZFQD010000157        11370 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.157, whole genome shotgun sequence.
ACCESSION   DAZFQD010000157 DAZFQD010000000
VERSION     DAZFQD010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11370
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.157"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            210..509
                     /gene="yoaC"
                     /locus_tag="ACHKTW_004380"
     CDS             210..509
                     /gene="yoaC"
                     /locus_tag="ACHKTW_004380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HFO6465290.1"
                     /translation="MPAVIDKALDFIGAMDVSAPTPSSMNESTAKGIFKYLKELGVPA
                     SAADITARADQEGWNPGFTEKMVGWAKKMESGERTVIKNPEYFSTYMQEELKALV"
     gene            complement(629..808)
                     /gene="yoaH"
                     /locus_tag="ACHKTW_004381"
     CDS             complement(629..808)
                     /gene="yoaH"
                     /locus_tag="ACHKTW_004381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HFO6465291.1"
                     /translation="MFAGLPSLTHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGERIVARFEDEDE"
     gene            882..2243
                     /gene="pabB"
                     /locus_tag="ACHKTW_004382"
     CDS             882..2243
                     /gene="pabB"
                     /locus_tag="ACHKTW_004382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HFO6465292.1"
                     /translation="MKTLSPAVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVADPICTLTTFGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTREQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITARLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            2247..2825
                     /gene="nudL"
                     /locus_tag="ACHKTW_004383"
     CDS             2247..2825
                     /gene="nudL"
                     /locus_tag="ACHKTW_004383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HFO6465293.1"
                     /translation="MEYRSLTLDYFLSRFQLLRPQINRETLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVKP"
     gene            3009..4373
                     /gene="sdaA"
                     /locus_tag="ACHKTW_004384"
     CDS             3009..4373
                     /gene="sdaA"
                     /locus_tag="ACHKTW_004384"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFO6465294.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVEERERLLLAQGR
                     HEVDFPRDNGMCFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            4504..6102
                     /gene="pdeD"
                     /locus_tag="ACHKTW_004385"
     CDS             4504..6102
                     /gene="pdeD"
                     /locus_tag="ACHKTW_004385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFO6465295.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSEVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRALIKGSPVLIQWTPAAGSSN
                     AGVMEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQRFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTR
                     YVMAETIRQRHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMPLR"
     gene            complement(6106..7662)
                     /gene="yoaE"
                     /locus_tag="ACHKTW_004386"
     CDS             complement(6106..7662)
                     /gene="yoaE"
                     /locus_tag="ACHKTW_004386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HFO6465296.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPMPI
                     PEGAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLF
                     PVCRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRAR
                     GSFVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGDGWLVKGGTDLHALQ
                     QALDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIIEANDYRVDLVRIV
                     KEQPAHDEDE"
     gene            complement(7650..7808)
                     /gene="yoaL"
                     /locus_tag="ACHKTW_004387"
     CDS             complement(7650..7808)
                     /gene="yoaL"
                     /locus_tag="ACHKTW_004387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaL"
                     /protein_id="HFO6465297.1"
                     /translation="MDRHRRHFSIRPFNACLSGSLCRTFRLHFVVTPALFLASNSYSL
                     SRSLSWNS"
     gene            8124..9095
                     /gene="manX"
                     /locus_tag="ACHKTW_004388"
     CDS             8124..9095
                     /gene="manX"
                     /locus_tag="ACHKTW_004388"
                     /EC_number="2.7.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HFO6465298.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            9158..9958
                     /gene="manY"
                     /locus_tag="ACHKTW_004389"
     CDS             9158..9958
                     /gene="manY"
                     /locus_tag="ACHKTW_004389"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HFO6465299.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            9971..10822
                     /gene="manZ"
                     /locus_tag="ACHKTW_004390"
     CDS             9971..10822
                     /gene="manZ"
                     /locus_tag="ACHKTW_004390"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HFO6465300.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVSR
                     ITDQTGKEHVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            10877..11335
                     /gene="yobD"
                     /locus_tag="ACHKTW_004391"
     CDS             10877..11335
                     /gene="yobD"
                     /locus_tag="ACHKTW_004391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 family protein"
                     /protein_id="HFO6465301.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRVPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKIYKLLVSTQ"
BASE COUNT         2785 a         2868 c         2865 g         2852 t
ORIGIN      
        1 tcttagctga ataatctggt tatagcgtga ggactgaccg ttaaatttct ccatcttact
       61 ggataacaca ccatgagctg ctacctttaa aacacctgaa tgtcaacagg ttaactcgtg
      121 ccatatcgtt ataatccctt tgatattaga tgcaaattaa ggtcatatat agtcttatcg
      181 ataacatggt taattttaag gataagaata tgcctgctgt aatagataaa gccctggatt
      241 tcattggtgc catggatgta tcagcgccaa caccaagttc gatgaatgaa agcacagcga
      301 agggaatctt taaatattta aaagaactgg gagtacccgc gagtgccgct gatattacgg
      361 cgcgagctga ccaggaaggc tggaaccctg ggttcacgga aaaaatggtt ggatgggcaa
      421 aaaaaatgga gtctggtgaa cgcactgtaa ttaaaaatcc tgaatacttt tcaacatata
      481 tgcaggaaga actaaaagca ctggtctgag cttaatttat atcagcataa atgggtcagg
      541 acgcttttaa tcacatataa aaagcgtcct ttttctcaca atcaaataat catcggattc
      601 tataacgaaa tcgatacgga ttagtgtgtt actcgtcttc atcctcaaaa cgcgccacga
      661 ttcgctcgcc ggagtggttg gcacgcagct cttctgccac cagcgcgatt gcctggccgc
      721 tgctcatccc ttgtgccatc agttcctgga tccgctcgac agctttttgc tgctgttcat
      781 gggtgagtga aggtaaacct gcaaacattg ttaactcctg ctaaattgtt ggcgctaatt
      841 atttcatgct acccggcaca tagccagtag agtcaggact gatgaagacg ttatctcccg
      901 ctgtgattac tttaccctgg cgtcaggacg ccgctgaatt ttatttctcc cgcttaagcc
      961 acctgccgtg ggcgatgctt ttacactccg gctatgccga tcatccgtat agccgctttg
     1021 atattgtggt cgccgatccg atttgcactt taaccacttt cggtaaagaa accgttgtta
     1081 gtgaaagcga aaaacgcaca acgaccactg atgacccgct acaggtgctc cagcaggtgc
     1141 tggatcgcgc agacattcgc ccaacgcata acgaagattt gccatttcag ggcggcgcac
     1201 tggggttgtt tggctacgat ctgggccgcc gttttgagtc actgccagaa attgcggaac
     1261 aagatatcgt tctgccggat atggcagtgg gtatctacga ttgggcgctc attgtcgacc
     1321 accagcgtca tacagtttct ttgctgagtc ataatgatgt caatgcccgt cgggcctggc
     1381 tggaaagcca gcaattctcg ccgcaggaag atttcacgct cacttccgac tggcaatcca
     1441 atatgacccg cgagcagtac ggcgaaaaat ttcgccaggt acaggaatat ctgcacagcg
     1501 gtgattgcta tcaggtgaat ctcgcccagc gttttcatgc gacctattct ggcgatgaat
     1561 ggcaggcatt ccttcagctt aatcaggcca accgcgcgcc atttagcgct tttttacgtc
     1621 ttgaacaggg tgcaatttta agcctttcgc cagagcggtt tattctttgt gataatagtg
     1681 aaatccagac ccgcccgatt aaaggcacgc taccacgcct gcccgatcct caggaagata
     1741 gcaaacaagc agaaaaactg gcgaactcag cgaaagatcg tgccgaaaat ctgatgattg
     1801 tcgatttaat gcgtaatgat atcggtcgtg ttgccgtagc cggttcggta aaagtaccag
     1861 agctgttcgt ggtggaaccc ttccctgccg tgcatcatct ggtcagcact ataacggcgc
     1921 gactaccaga acagttacac gccagcgatc tgctgcgcgc agcttttcct ggtggctcaa
     1981 taaccggggc tccgaaagta cgggctatgg aaattatcga cgaactggaa ccgcagcgac
     2041 gtaatgcctg gtgcggcagc attggctatt tgagcttttg cggcaacatg gataccagca
     2101 ttactatccg cacgctgact gccattaacg gacaaatata ctgctctgcg ggcggtggaa
     2161 ttgtcgccga tagccaggaa gaagcggaat atcaggaaac ttttgataaa gttaataaga
     2221 tattacgcca actggagaag taagacgtgg aataccgtag cctgacgctt gattattttt
     2281 tatctcgctt tcaacttttg cgcccgcaaa ttaaccggga aaccctaaat catcgtcagg
     2341 ctgccgtgtt aatccccatc gtccgtcgac cgcaaccggg gttgttgctg actcagcgtt
     2401 caattcatct gcgtaaacac gctggacaag tggcattccc tggaggtgca gtcgatgaca
     2461 cggacgcatc agttatcgcc gccgcgctgc gcgaagctga agaagaggtc gctataccgc
     2521 cttccgccgt tgaagttatc ggcgtgctgc cgcccgtcga tagcgtaact ggctatcagg
     2581 taaccccagt ggtcggcatt atcccgcccg atctgccgta tcgcgccagt gaagatgaag
     2641 tctcggcggt gtttgaaatg ccgctcgccc aggcattaca tctgggtcgt tatcaccctt
     2701 tagatatcta ccgccgtggc gattcacatc gggtatggct gtcctggtac gaacagtatt
     2761 ttgtatgggg aatgaccgca ggcataattc gtgagctggc gctgcaaatt ggtgtgaaac
     2821 cctgactata cttatcttta catctacaaa acactacttg agacaatcat cgcaatatta
     2881 gttaaatcgc ggtttttgat tagtttaatt catgtgaata gttaagccag tcgccgcgtt
     2941 ccctcttaca ctatgcgctg ttattagttc gttactggaa gtccagtcac cttgtcagga
     3001 gtattatcgt gattagtcta ttcgacatgt ttaaggtggg gattggtccc tcatcttccc
     3061 ataccgtagg gcctatgaag gcgggtaaac agttcgtcga tgatctggtc gaaaaaggct
     3121 tactggatag cgttactcgc gttgccgtgg acgtttatgg ttcactgtcg ctgacgggta
     3181 aaggccacca caccgatatc gccattatta tgggtcttgc aggtaacgaa cctgccaccg
     3241 tggatatcga cagtattccc ggttttattc gcgacgtaga agagcgcgaa cgtctgctgc
     3301 tggcacaggg acggcatgaa gtggatttcc cgcgcgacaa cgggatgtgt tttcataacg
     3361 gcaacctgcc gctgcatgaa aacggtatgc aaatccacgc ctataacggc gatgaagtcg
     3421 tctacagcaa aacttattat tccatcggcg gcggttttat cgtcgatgaa gaacactttg
     3481 gtcaggatgc tgccaacgaa gtgagcgtgc cgtatccgtt caaatctgcc accgaactgc
     3541 tcgcgtactg taatgaaacc ggctattcgc tgtctggtct cgctatgcag aacgaactgg
     3601 cgctgcacag caagaaagag atcgacgagt atttcgcgca tgtctggcaa accatgcagg
     3661 catgtatcga tcgcgggatg aacaccgaag gtgtactgcc aggcccgctg cgcgtgccac
     3721 gtcgtgcgtc tgccctgcgc cggatgctgg tttccagcga taaactgtct aacgatccga
     3781 tgaatgtcat tgactgggta aacatgtttg cgctggcagt taacgaagaa aacgccgccg
     3841 gtggtcgtgt ggtaactgcg ccaaccaacg gtgcctgcgg tatcgttccg gcagtgctgg
     3901 cttactatga ccactttatt gaatcggtca gcccggacat ctatacccgt tactttatgg
     3961 cagcgggcgc gattggtgca ttgtataaaa tgaacgcctc tatttccggt gcggaagttg
     4021 gttgccaggg cgaagtgggt gttgcctgtt caatggctgc tgcgggtctt gcagaactgc
     4081 tgggcggtag cccggaacag gtttgcgtgg cggcggaaat tggcatggaa cacaaccttg
     4141 gtttaacctg cgacccggtt gcagggcagg ttcaggtgcc gtgcattgag cgtaatgcca
     4201 ttgcctctgt gaaggcgatt aacgccgcac ggatggctct gcgccgcacc agtgcaccgc
     4261 gcgtctcgct ggataaggtc atcgaaacga tgtacgaaac cggtaaggac atgaacgcca
     4321 aataccgcga aacctcacgc ggtggtctgg caatcaaagt ccagtgtgac taatacttct
     4381 tactcgccca tctgcaacgg atgggcgaat ttatacccgc tttctcgtct gctgtaatat
     4441 tccccactac acttccactg ttgcgtcagg cgtttgtcgc catacgctta cagggtggcc
     4501 cgcatgcaaa aagcacaacg gatcattaaa acctatcgcc gtaatcgaat gattgtttgt
     4561 acgatttgcg cactcgttac gctcgcttcg accctgagcg tgcgatttat ttcacagcgt
     4621 aacttaaatc aacaacgggt agtacaattc gccaatcacg ctgtagagga attagataaa
     4681 gtactgcttc ccctacaggc aggtagcgaa gtcttgcttc cgctgattgg tctgccctgc
     4741 tctgtcgccc atttgccatt acgtaaacag gcggcaaaac tccaaactgt gcgatccatt
     4801 ggcctggtgc aagacggcac actttattgc tccagcattt ttggttatcg caatgtgccc
     4861 gtcgtggaca ttctggctga acttcctgca ccgcaaccac ttttacgcct gacgatcgac
     4921 cgtgccctga ttaaaggcag tccggttttg attcaatgga cgcctgcagc gggcagtagc
     4981 aatgctgggg tcatggagat gattaacatc gacttactga cggcaatgct ccttgagcca
     5041 caactgccgc aaatcagtag cgccagcctg acggtggaca aacggcattt gctctatggt
     5101 aatgggctgg tagattccct tccgcaacct gaagacaatg aaaactatca ggtttcttcg
     5161 caacgctttc cttttaccat taacgttaat ggtccggggg ctacggcgct ggcatggcac
     5221 tatcttccaa cacaattacc gctggcggtg ctgctaagtt tactggtggg ctacatcgcc
     5281 tggctggcga ccgcttaccg gatgagcttt tcccgcgaaa tcaatctggg cctggcgcaa
     5341 catgagttcg aattgttctg tcagcctttg cttaatgcgc gcagccagca atgtattggt
     5401 gtagagattt tgctgcgctg gaacaatccg cgtcagggct ggatttcacc ggatgtgttt
     5461 attcctatcg cggaagaaca tcatttaatt gtgccactga cccgctatgt gatggcagaa
     5521 accattcgtc agcgccatgt tttcccgatg agtagtcagt ttcatgttgg cattaacgtc
     5581 gcacccagcc attttcgccg tggtgtgctg ataaaagatc tcaatcagta ctggtttagc
     5641 gctcacccga ttcagcaact gatcctcgaa atcaccgaac gcgatgcctt actggatgtt
     5701 gattatcgga ttgcccgcga gctacatcgt aaaaacgtca aactggcgat tgatgacttc
     5761 ggcaccggca acagttcatt ttcctggctt gaaacattac gtcctgacgt gctgaaaatt
     5821 gataagtcat ttaccgcagc tataggttct gacgcggtta actcgacggt gaccgatatc
     5881 atcatcgcgc tggggcaaag actgaatatt gaactggtgg cggagggcgt ggaaacgcaa
     5941 gaacaggcga agtatttgcg ccgtcatggc gtgcatattt tgcaagggta tttgtacgca
     6001 cagccgatgc cgctacgtga ttttcccaaa tggctggcgg gcagccaacc gccgcccgcc
     6061 cggcataatg gacatatcac gcccattatg ccgttacgtt aacgcttact catcttcatc
     6121 gtgcgccggt tgctctttaa caatgcgaac cagatcaaca cgataatcat tggcttcaat
     6181 gatggtgata tgcagtggcc ctacatcaat cacatcgccc acacggggaa tgtgaccatt
     6241 tgccgagatc accaggcccg cgaccgtcgc gatatcatcg tcatcggcaa ggtgctcaac
     6301 atcaagcgcc tgctgcaagg catgcaaatc tgtaccgcct tttaccagcc agccgtcacc
     6361 atcggtaatg atttccggcg tttcgtcagc gtccgggaat tcacccgcaa tggcttccag
     6421 cacatccagc ggcgtgacca agccttgtac cacaccaaac tcgttggtca cgataacaaa
     6481 gctcccgcga gcgcgacgca gcacgcccag caggttgatc ggatcgaggg tttccgggac
     6541 gataatcgcc ggagacgccg aagcaatcgc cgccacatca acgccctctt ccagcgccac
     6601 cagcagttct ttagcacgta cgattccgat gatttcatcc agttcaccgc gacataccgg
     6661 gaacaggctg tgcggtgagg agagcagctg ctcgcggatt tcatcgaccc cgagattagc
     6721 gtcaacccag cttatttcac cgcgcggcgt catgatcccg cgcagagaac gcgacgccag
     6781 cgtcagtacg ccgttaatca tgtaacgttc ttcttcggca aatgcacctt ccgggatcgg
     6841 catcggcatc gggttatcgg catcgtgctg aacattggcc tgacgtttcc cgcccatcaa
     6901 acgcaggatg gcatcggcag tacgcgctcg cagcggcaaa gtcgactggt gacgaataaa
     6961 gttgcgacgc gcaatctggt taaacacttc gatgatgatc gagaagccaa tcgcggcata
     7021 caggtaacct ttcggaatgt ggaaaccgaa accttctgcc accagactca gaccaatcat
     7081 taacaggaag ctcagacaga gcaccaccac cgtggggtgc tggttaacga atcgcgtcag
     7141 cggtttggat gccagcaaca taaccgccat cgcaatcact accgccgcca tcatcaccgg
     7201 cagatggtta accatcccta ctgcagtaat taccgcatcc aacgagaaga cggcgtcaag
     7261 gatgacaatc tgtgtgacga ccacccagaa actggcgtag cctttaccgt ggccggaatc
     7321 atgatcgcgg ttttccagcc gttcatgcag ttcggttgtt gctttgaaca gcaagaatat
     7381 ccccccgaac aacataatca ggtcgcgtcc ggagaaggag aaatccatga cggtaaatag
     7441 cggtttggtc agcgtgacca tccatgaaat cagcgacagc agccccagac gcataatcag
     7501 cgccagtgat aaccccagca aacgcgcttt atcgcgttgt tttggcggca gtttgtcagc
     7561 aagaatggcg atgaagacca ggttatcgat acccagcaca atttcgagaa caacaagcgt
     7621 gagtagcccc gcccaaattg aggggtccat taagaattcc atgacaagct cctgcttaag
     7681 gaatagctat tcgacgccag aaataatgca ggcgtaacga caaaatgcaa acgaaaggtg
     7741 cggcatagag agccagaaag gcaggcgtta aaaggcctga tgctgaaatg acgtcggtga
     7801 cgatccatac tgcgggctac tgccctatac tccatggttg ttaaacggga gttaaacata
     7861 tcagagacgc ctctgatttg gcaaagattt accttccttt gcaaacgaat gtgacaagga
     7921 tattttacct ttcgaaattt ctgctaatcg aaagttaaat tacggatctt catcacataa
     7981 aataattttt tcgatatcta aaataaatcg cgaaacgcag gggtttttgt tgtagccctt
     8041 atctgaatcg attcgattgt ggacgacgat tcaaaaatac atctggcacg ttgaggtgtt
     8101 aacgataata aaggaggtag caagtgacca ttgctattgt tataggcaca catggttggg
     8161 ctgcagagca gttgcttaaa acggcagaaa tgctgttagg cgagcaggaa aacgtcggct
     8221 ggatcgattt cgttccaggt gaaaatgccg aaacgctgat tgaaaagtac aacgctcagt
     8281 tggcaaaact cgacaccact aaaggcgtgc tgtttctcgt tgatacatgg ggaggcagcc
     8341 cgttcaatgc tgccagccgc attgtcgtcg acaaagagca ttatgaagtc attgcaggcg
     8401 ttaacattcc aatgctcgtg gaaacgttaa tggcccgtga tgatgaccca agctttgatg
     8461 aactggtggc actggcagta gaaacaggcc gtgaaggcgt gaaagctctg aaagccaaac
     8521 cggttgaaaa agccgcgcca gcacccgctg ccgcagcacc aaaagcggct ccaactccgg
     8581 caaaaccaat ggggccaaac gactacatgg ttattggcct tgcgcgtatc gacgaccgtc
     8641 tgattcacgg tcaggtcgcc acccgctgga ccaaagaaac caatgtctcc cgtattattg
     8701 ttgttagtga tgaagtggct gcggataccg ttcgtaagac actgctcacc caggttgcac
     8761 ctccgggcgt aacagcacac gtagttgatg ttgccaaaat gattcgcgtc tacaacaacc
     8821 cgaaatatgc tggcgaacgc gtaatgctgt tatttaccaa cccaacagat gtagagcgtc
     8881 tcgttgaagg cggcgtgaaa atcacctctg ttaacgtcgg tggtatggca ttccgtcagg
     8941 gtaaaaccca ggtgaataac gcggtttcgg ttgatgaaaa agatatcgag gcgttcaaga
     9001 aactgaatgc gcgcggtatt gagctggaag tccgtaaggt ttccaccgat ccgaaactga
     9061 aaatgatgga tctgatcagc aaaatcgata agtaacgtat tgtgttgatt atcactcagt
     9121 tttcacactt aagtcttacg taaacaggag aagtacaatg gagattacca ctcttcaaat
     9181 tgtgctggta tttatcgtag cctgtatcgc aggtatggga tcaatcctcg atgaatttca
     9241 gtttcaccgt ccgctaatcg cgtgtaccct ggtgggtatc gttcttgggg atatgaaaac
     9301 cggtattatt atcggtggta cgctggaaat gatcgcgctg ggctggatga acatcggtgc
     9361 tgcagttgcg cctgacgccg ctctggcttc tatcatttct accattctgg ttatcgcagg
     9421 tcatcagagc attggtgcag gtatcgcact ggcaatccct ctggccgctg cgggccaggt
     9481 actgaccatc atcgttcgta ctattaccgt tgctttccag cacgctgcgg ataaggctgc
     9541 tgataacggc aacctgacag cgatttcctg gatccacgtt tcttctctgt tcctgcaagc
     9601 aatgcgtgtg gctattccgg ccgtcatcgt tgcgctgtct gttggtacca gcgaagtaca
     9661 gaacatgctg aatgcgattc cggaagtggt gaccaatggt ctgaatatcg ccggtggcat
     9721 gatcgtggtg gttggttatg cgatggttat caacatgatg cgtgctggct acctgatgcc
     9781 gttcttctac ctcggcttcg taaccgcagc attcaccaac tttaacctgg ttgctctggg
     9841 tgtgattggt actgttatgg cagtgctcta catccaactt agcccgaaat acaaccgcgt
     9901 agccggtgcg cctgctcagg cagctggtaa caacgatctc gataacgaac tagactaaca
     9961 ggtgagcgaa atggttgata caactcaaac taccaccgag aaaaaactca ctcaaagtga
    10021 tattcgtggc gtcttcctgc gttctaacct cttccagggt tcatggaact tcgaacgtat
    10081 gcaggcactg ggtttctgct tctctatggt accggcaatt cgtcgcctct accctgagaa
    10141 caacgaagct cgtaaacaag ctattcgccg tcacctggag ttctttaaca cccagccgtt
    10201 cgtggctgcg ccgattctcg gcgtaaccct ggcgctggaa gaacagcgtg ctaatggcgc
    10261 agagatcgac gacggtgcta tcaacggtat caaagtcggt ttgatggggc cgctggctgg
    10321 tgtaggcgac ccgatcttct ggggaaccgt acgtccggta tttgcagcac tgggtgccgg
    10381 tatcgcgatg agcggcagcc tgttaggtcc gctgctgttc ttcatcctgt ttaacctggt
    10441 gcgtctggca acccgttact acggcgtagc gtatggttac tccaaaggta tcgatatcgt
    10501 taaagatatg ggtggtggct tcctgcaaaa actgacggaa ggggcgtcta tcctcggcct
    10561 gtttgtcatg ggggcattgg ttaacaagtg gacacatgtc aacatcccgc tggttgtctc
    10621 tcgcattact gaccagacgg gcaaagaaca cgttactact gtccagacta ttctggacca
    10681 gttaatgcca ggcctggtac cactgctgct gacctttgct tgtatgtggc tactgcgcaa
    10741 aaaagttaac ccgctgtgga tcatcgttgg cttcttcgtc atcggtatcg ctggttacgc
    10801 ttgcggcctg ctgggactgt aagactgttg tacactaccg gggccttttg gccccatttt
    10861 tttatctgga ggattaatga caatcacgga cctggtactg attcttttca tcgccgcact
    10921 cctggccttc gcgatctacg atcagttcat catgccccgc cgtaacggcc ccaccctgct
    10981 ggcaattcct ttgctccggc gtggtcgcat cgatagcgtt atcttcgtcg gattgattgt
    11041 cattcttatc tataacaacg tcacgaatca tggtgcgtta ataacgacat ggttattaag
    11101 cgcactggct ctgatgggtt tttatatatt ctggatccgc gttccgaaga tcatctttaa
    11161 acaaaaaggt tttttcttcg ccaatgtctg gattgaatat agccgaatca aagcgatgaa
    11221 cttgtcggaa gatggcgtgt tggtgatgca attagaacag cgtcggctgt taatccgcgt
    11281 tcgaaatatc gacgatctgg aaaaaattta taagcttctc gtttcaactc aataagttat
    11341 gaatttagcc aaagctatgt ttagtgtatt
//
LOCUS       DAZFQD010000158        11338 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.158, whole genome shotgun sequence.
ACCESSION   DAZFQD010000158 DAZFQD010000000
VERSION     DAZFQD010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11338
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.158"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            24..785
                     /gene="ydeO"
                     /locus_tag="ACHKTW_004392"
     CDS             24..785
                     /gene="ydeO"
                     /locus_tag="ACHKTW_004392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HFO6465302.1"
                     /translation="MSLVCSVIFIHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPY
                     ERHFKEIGFTENTIKKYLQCTNIQTVTVPVPAKFLRASNVPTGLLNEMIAYLNSEERN
                     HHNFSELLLFSCLSIFAACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCL
                     YISESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYA
                     FRKHFGNSPKRVSKEYRCQSHTGMNTGNTMSALAI"
     gene            1187..2869
                     /gene="ydeN"
                     /locus_tag="ACHKTW_004393"
     CDS             1187..2869
                     /gene="ydeN"
                     /locus_tag="ACHKTW_004393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFO6465303.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     SDFTPTEYSTKGKPNIIVLTMDDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAI
                     EAAQKSTPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGI
                     PLTETFLPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTFSAEEWQPQ
                     NRGFDYFMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPF
                     MLYLAYNAPHLPNDNPAPDQYQKQFNTGSQTADNYYASVYSVDQGVKRILEQLKKNGQ
                     YDNTIILFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKL
                     ISAMDFYPTALDAADISIPKDLKLDGVSLLPWLQDKKQGEPHKNLTWITSYSHWFDEE
                     NIPFWDNYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVENNQLGLYKLTD
                     LQQKDNLAAANPQVVKEMQGVVREFIDSSQPPLSEVNQEKFNNIKKALSEAK"
     gene            2921..4078
                     /gene="ydeM"
                     /locus_tag="ACHKTW_004394"
     CDS             2921..4078
                     /gene="ydeM"
                     /locus_tag="ACHKTW_004394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416014.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HFO6465304.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIAASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQCYAGQKRIFNALQTNGILLNNEW
                     CAFLKEHEFLVGISIDGPQELHDRYRRSNSGNGTFAKVIAAIERLKSYQVEFNTLTVI
                     NNVNVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKIGNINKSELKTMNSVQLTAQKKRISAKCLKCAYKPICNGGCPKHRIT
                     KVNNETVSYFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(4044..4166)
                     /locus_tag="ACHKTW_004395"
     CDS             complement(4044..4166)
                     /locus_tag="ACHKTW_004395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6465305.1"
                     /translation="MTKHPTGIYVGCLVKVIRRRLRMELKESVINYSPFVLQHP"
     gene            4369..6054
                     /gene="yddA"
                     /locus_tag="ACHKTW_004396"
     CDS             4369..6054
                     /gene="yddA"
                     /locus_tag="ACHKTW_004396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HFO6465306.1"
                     /translation="MITIPITFCMLIAKYLCLLKPFWLRKNNKTSVLLIIIILAMILG
                     VVKIQVWLNDWNNDFFNALSQKETDKLWQLVLWFPALLGIFVLISVNKTWLIKLLTIR
                     WREWLTDYYLNRWFADKNYYFTQIYGEHKNTDNPDQRIAEDILLLISKTLSLSFGFIQ
                     SLSMLITFTVILWQSAGTLSFTVGGTEWNIQGYMVYTVVLIVIGGTLFTHKVGKRIRP
                     LNVEKQRSEATFRTNLVQHNKQAELIALSNAESLQRQELSDNFHTIKENWHRLMNRQR
                     WLDYWQNIYSRSLSVLPYFLLLPQFISGQINLGGLMKSRQAFMLVSNNLSWFIYKYDE
                     IAELAAVIDRLYEFHQLTEQRPTNKPKNCQHAVQVADASIRTPDNKIILENLNFHVPP
                     GKWLLLKGYSGAGKTTLLKTLSHCWPWFKGDISSPADSWYVSQTPLIKTGLLKEIICK
                     ALPLPVDDKSLSEILHQVGLGKLAARIHDHDRWGDILSSGEKQRIALARLILRRPKWI
                     FLDETTSHLEEQEAIRLLRLVREKLPTSGVIMVTHQPGVWNLADDICDISAVL"
     gene            6092..8466
                     /gene="yddB"
                     /locus_tag="ACHKTW_004397"
                     /pseudo
     CDS             6092..8466
                     /gene="yddB"
                     /locus_tag="ACHKTW_004397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310127.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
     gene            8523..11306
                     /gene="pqqL"
                     /locus_tag="ACHKTW_004398"
     CDS             8523..11306
                     /gene="pqqL"
                     /locus_tag="ACHKTW_004398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFO6465307.1"
                     /translation="MRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLGNGLRYMI
                     YPHAHPKDQVNLWLQIHTGSLQEEDNERGVAHFVEHMMFNGTKTWPGNKVIETFESMG
                     LRFGRDVNAYTSYDETVYQVSLPTTQKQNLQQVMAIFSEWSNAATFEKLEVDAERGVI
                     TEEWRAHQDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQP
                     NNMTFIVVGDIDSKEALALIKDNLSKLPANKAAENRVWPTKAENHLRFNIINDKENRV
                     NGIALYYRLPMVQVNDEQSFIEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVK
                     IAPDYQSLFFRVNARDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAV
                     DQQAERDLRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQ
                     DAFWEQMVNNELAAKKALSPAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQAEIS
                     SKETLAENLTSLTLSNGARVILAKSAGEEQKLQITAVSNKGDLSFPAQQKSLIALANK
                     AVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRIT
                     HSTINDNIWASLQNAQIQALKTLDQRPAEKFAQQMYETRYADDRTKLLQENQIVQFTA
                     ADALAADRQLFSSPADITFVIVGNVSEDKLVALITRYLGSIKHSDSPLAAGKPLTRAT
                     DNASVTVKEQNEPVAQVSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIREQASG
                     AYSVSSRLSVDPQAKDISHLLAFTCQPERHDELLTLANEVMVKRLAKGISEQELNEYQ
                     QNVQRSLDIQQRSVQQLANTIVNSLIQYDDPAAWTEQEQLLKQMTVENVNTAVKQYLS
                     HPVNTYTGVLLPK"
BASE COUNT         3334 a         2479 c         2455 g         3070 t
ORIGIN      
        1 ctttattgtt ttaaggagat aaaatgtcgc tcgtttgttc tgttatattt attcatcatg
       61 ccttcaacgc taacatttta gataaagatt acgccttctc tgacggcgag atcctgatgg
      121 tagataacgc tgttcgtacg cattttgaac cttatgagcg gcattttaaa gagatcggat
      181 ttactgaaaa taccattaaa aaatatctac aatgcactaa catccagaca gtgacggtgc
      241 ctgttcctgc gaagttttta cgtgcttcaa atgtaccgac tggattgctt aatgaaatga
      301 ttgcttatct caactcggaa gaacgcaatc atcataattt ttcagaactt ttgctttttt
      361 cttgcctgtc tatttttgcc gcatgcaaag gtttcattac actattaact aacggtgtgc
      421 tatccgtttc cggaaaagtg agaaatattg tcaacatgaa gctggcgcac ccatggaagc
      481 tgaaagatat ttgtgactgc ctgtacatca gtgaaagcct gttgaagaaa aaactaaagc
      541 aagagcaaac gacattctca cagattctat tagatgccag aatgcagcac gcaaaaaatt
      601 tgatacgcgt agaaggttca gtcaataaaa ttgccgaaca atgtggttat gccagtacat
      661 cttattttat ttatgcgttc cgcaaacatt tcggcaacag tccgaagaga gtttctaagg
      721 agtaccgttg tcaaagtcac acgggtatga atacgggcaa cacgatgagt gctttagcta
      781 tttgattatt tgctaacgag tagtcaacca cgcacgctac gtaagaatta tatggggcag
      841 ccattccctg ccccgcgttg tttttaggcg atatatttat tgaaataaat aagtgacatc
      901 catcacatat ttatgcactt gcataacctg ttgcatgatt atttatgatc tcaattcggc
      961 attttgtcag taaaatgcaa taatttatta aatatcaata aattagttgt ttatcggcga
     1021 gaaattactt aatagaacag aaagtaatgt caacgcttta tggactgtta tttccctttt
     1081 tttagctaaa tctgctatct ctttatgtga ctaacttcac ttacatccac ttatttctct
     1141 tcgtaaaatt acttcggaat taagtacaat aagaagagga acatttatga agtctgcatt
     1201 aaagaaaagt gtcgtaagta cctcgatatc tttgatactg gcatctggta tggctgcatt
     1261 tgctgctcat gcggcagatg atgtaaagct gaaagcaacc aaaacaaacg ttgctttctc
     1321 agactttacg ccgactgaat acagtaccaa aggaaagcca aatattatcg tactaaccat
     1381 ggatgatctt ggttatggac aacttccttt tgataaggga tcttttgacc caaaaacaat
     1441 ggaaaatcgt gaagttgtcg atacctacaa aatagggata gataaagcca ttgaagctgc
     1501 acaaaaatca acgccgacgc tcctttcatt aatggatgaa ggcgtacggt ttactaacgg
     1561 ctatgtggca cacggtgttt ccggcccctc ccgtgccgca ataatgaccg gtcgagctcc
     1621 cgcccgcttt ggtgtctatt ccaataccga tgctcaggat ggtattccgc taacagaaac
     1681 tttcttgcct gaattattcc agaatcatgg ttattacact gcagcagtag gtaaatggca
     1741 cttgtcaaaa atcagtaatg tgccggtacc ggaagataaa caaacgcgtg actatcatga
     1801 caacttcacc acattttctg cggaagaatg gcaacctcaa aaccgtggct ttgattactt
     1861 tatgggattc cacgctgcag gaacggcata ttacaactcc ccttcactgt tcaaaaatcg
     1921 tgaacgtgtc cccgcaaaag gttatatcag cgatcagtta accgatgagg caattggcgt
     1981 tgttgatcgt gccaaaacac ttgaccagcc ttttatgctt tacctggctt ataatgctcc
     2041 gcacctgcca aatgataatc ctgcaccgga tcaatatcag aagcaattta ataccggtag
     2101 tcaaacagca gataactact acgcttccgt ttattctgtt gatcagggtg taaaacgcat
     2161 tctcgaacaa ctgaagaaaa acggacagta tgacaataca attattctct ttacctccga
     2221 taatggtgcg gttatcgatg gtcctctgcc gctgaacggg gcgcaaaaag gctataagag
     2281 ccagacctat cctggcggta ctcacacccc aatgtttatg tggtggaaag gaaaacttca
     2341 acccggtaat tatgacaagc tgatttccgc aatggatttc tacccgacag ctcttgatgc
     2401 agccgatatc agcattccaa aagaccttaa gctggatggc gtttccttgc tgccctggtt
     2461 gcaagataag aaacaaggcg agccacataa aaatctgacc tggataacct cttattctca
     2521 ctggtttgac gaggaaaata ttccattctg ggataattac cacaaatttg ttcgccatca
     2581 gtcagacgat tacccgcata accccaacac tgaggactta agccaattct cttatacggt
     2641 gagaaataac gattattcgc ttgtgtatac agtagaaaac aatcagttag gtctctacaa
     2701 actgacggat ctacagcaaa aagataacct tgccgccgcc aatccgcagg tcgttaaaga
     2761 gatgcaaggc gtggtaagag agtttatcga cagcagccag ccaccgctta gcgaagtaaa
     2821 tcaggagaag tttaacaata tcaagaaagc actaagcgaa gcgaaataac taaaccttca
     2881 tgcggcggaa ttttccgccg ccttattgag cgagatagcg atgcacgtta cagccaagcc
     2941 ctccagtttt caatgtaatc tcaaatgtga ttactgtttt taccttgaaa aagagtcgca
     3001 gtttactcat gaaaaatgga tggatgacag cactctgaaa gagttcatca aacaatatat
     3061 cgcagcgtct ggcaatcagg tctattttac ctggcaaggc ggtgaaccca ctctggctgg
     3121 cctggatttt ttccgtaaag ttattcacta tcaacaatgc tatgcaggcc aaaaacgtat
     3181 ttttaatgca ttacaaacga atggcatttt attgaataat gaatggtgtg cttttctcaa
     3241 agaacatgag tttctggttg gtatctcgat cgatggcccc caggagctac atgaccgtta
     3301 cagacgcagt aattcaggta acggtacttt tgcaaaagtg atagcagcca tcgagcgtct
     3361 gaaatcatat caagttgagt ttaatacgtt aaccgtcatt aataacgtta atgtccatta
     3421 ccctcttgag gtttatcatt ttttaaaatc tatcggcagt aaacatatgc aatttatcga
     3481 attgctcgaa accgggacgc cgaatattga tttcagtggt catagtgaga acacattccg
     3541 tatcattgat ttttctgtgc ctcccacggc ttatggcaag tttatgtcaa ccatttttat
     3601 gcaatgggtt aaaaacgatg tgggtgaaat tttcatccgt cagtttgaaa gctttgtcag
     3661 ccgtttttta gggaatgggc ataccagttg tattttccag gagtcctgca aggataatct
     3721 ggttgttgaa agtaatggag atatttatga atgcgaccat tttgtctatc cacagtacaa
     3781 aattggaaac attaataaat ctgaactcaa aacgatgaac agtgtacaac tgacagcgca
     3841 aaaaaaacgg atttcagcga aatgtctgaa atgtgcatat aaacctatct gcaatggcgg
     3901 ttgtcctaag catcgtatta ctaaagtaaa caatgagact gtttcttatt tttgcgaagg
     3961 ttataaaatc cttttttcaa ccatggtacc ttatatgaac gccatggtgg agttagctaa
     4021 gaacagagta ccgctttacc acattatgga tgttgcaaaa caaatggaga ataattaata
     4081 acgctctctt ttaactccat tcttagcctc cttcttatga ccttaacaag gcacccgaca
     4141 taaattcctg tcgggtgttt cgtcatcatc atcttccctt gcgaaaatcc tggcttacgt
     4201 ggtatgcgtc agggcattag caataccctt gattatcaat agcataacca taaccactga
     4261 aaatgaaata ttcctttata tttcatattg ttaaatgata cttaaataaa tttcatcagt
     4321 aaatcattgc catagataat cattggatgg cacaatcaat tttcgttaat gataaccatt
     4381 cccattacgt tttgtatgtt gatagcaaag tatctctgtc tgctgaagcc cttttggctg
     4441 cgtaagaata ataaaacttc agtgttgtta atcattatta tcctcgccat gatccttggc
     4501 gtggttaaaa ttcaggtctg gttaaacgac tggaataatg attttttcaa tgcgctaagc
     4561 cagaaagaaa ccgataagct ttggcagctt gtcctttggt tccctgccct gctggggatc
     4621 tttgtattaa tatccgtaaa caaaacatgg ttaattaaac tactgactat tcgctggcgc
     4681 gaatggttaa cagattacta ccttaatcgg tggtttgcag ataaaaacta ctacttcacg
     4741 cagatctacg gcgagcataa aaatacagat aaccctgacc aacgtatcgc tgaagacatt
     4801 ctcttactga tcagcaaaac gctcagcctt tcatttggct tcatccagtc acttagcatg
     4861 ctgatcacct ttaccgttat tctttggcaa agcgcgggta cgctctcatt cactgttggt
     4921 ggaacagaat ggaatatcca ggggtatatg gtctataccg ttgtgctcat tgtgatcggt
     4981 ggaactttat ttacccataa ggttggtaaa cgtattcgtc cgcttaatgt ggaaaaacaa
     5041 cgtagcgaag cgacatttcg aactaatctt gtgcagcata ataaacaggc agaactgatt
     5101 gcgctaagca atgctgagtc tctacaacgc caggagttga gcgacaattt tcacaccatt
     5161 aaagagaact ggcatcgttt aatgaatcgt cagcggtggc ttgattactg gcagaatatt
     5221 tattcgcgtt cgcttagcgt tcttccgtac tttctgttat tgccgcagtt tattagcgga
     5281 cagatcaatc tgggcggact tatgaaatcg cgccaggcat ttatgctggt atcgaacaat
     5341 ttaagctggt ttatttataa atatgatgaa attgctgaac tggctgcggt tatcgatcgc
     5401 ttgtatgagt tccatcaact caccgaacag cgccctacga ataagcctaa aaattgccaa
     5461 catgcggtac aagtggctga cgcgagtatt cgtacccctg ataataagat catattagag
     5521 aacctgaact ttcatgttcc gccaggcaaa tggctattac tgaaaggtta ctctggtgcg
     5581 ggaaaaacca cactgcttaa aacattatcc cactgctggc cgtggtttaa aggtgatatt
     5641 tcttctcctg ccgacagttg gtatgtgtca caaacaccgt taatcaaaac cggcttactg
     5701 aaagagatta tttgtaaagc acttcctctg cccgtagacg ataaatcgtt gagcgaaata
     5761 ctgcatcagg ttggtctggg gaaattggct gcgcgtattc atgaccacga tcgctgggga
     5821 gatattcttt ccagcggcga aaaacaacgt atcgccctgg cacgattaat tttacgacgt
     5881 ccgaaatgga tatttcttga cgaaactacc tctcatcttg aggaacaaga ggctatccgc
     5941 ttactgcgtt tagtgcgtga aaaactaccc acaagcggcg tcattatggt tacacatcaa
     6001 cccggtgtct ggaacctggc cgatgatatt tgtgacatta gcgcggtttt ataaaaacaa
     6061 taaaagccct gctgtacgga gatataaata aatgaagcga gttcttattc ctggcgtcat
     6121 tttatgtggc gctgatgtgg cgcaggccgt cgatgacaaa aacatgtaca tgcatgtttt
     6181 tgaagagatg acggtctatg ctcctgtccc tgtacccgta aacggcaaca cgcattacac
     6241 cagtgaaagc atcgagcgtt taccgaccgg gaatggcaat atcagcgatc tgctgagaac
     6301 caaccctgcg gtacgcatgg attcaacgca aagtacctcg ttgaaccagg gagatattcg
     6361 cccggagaaa atctctattc acggtgcgtc gccctaccag aatgcctatt tgattgatgg
     6421 tattagtgcc actaataacc tgaacccagc gaatgagtcc gatgccagta gtgcaaccaa
     6481 tattagcggg atgtcacagg ggtattatct tgatgtcagc ttactggaca atgtgacgct
     6541 ttatgacagt tttgtgccgg ttgaattcgg tcgcttcaat ggcggggtaa ttgatgcaaa
     6601 gatcaaacgc ttcaacgctg atgatagcag cgtgaaactg ggttatcgca ctacgcgttc
     6661 ggactggtta acatcgcata tcgatgagaa taacaagagc gcatttaatc aaggttcttc
     6721 aggaagtact tattactctc cagattttaa aaagaacttt tataccttgt cgtttaatca
     6781 ggaactcgct gataactttg gcgttaccgc cggtttatcg cgccgccagt ctgatatcac
     6841 ccgcgcggat tatgtttcga atgacggcat tgtcgccggt cgggcacagt ataaaaacgt
     6901 tatcgatact gcattgagca aatttacctg gtttgccagc gaccgcttta cccacgattt
     6961 aaccttaaaa tataccggct ccagccgtga ttataatacc agcaccttcc cgcagtctga
     7021 tcgcgaaatg ggtaataaat cctatggtct ggcatgggat atggatacgc agctcgcatg
     7081 ggccaaactt cgtaccaccg ttggttggga tcatattagt gattataccc gtcacgatca
     7141 tgacacctgg tacaccgaac tttcatgtac atatggtgat attacagggc gttgcacccg
     7201 tggcggatta ggacacattt cccaggctgt agataattac accttcaaaa cacgcctgga
     7261 ctggcaaaaa ttcgccgcgg tggtaatgtt tcgcatcaac cctacttcgg cgcggaatac
     7321 atctattccg atgcatggac tgaacgccat aaccagtctg aatcctatgt gattaatgct
     7381 gccggaaaga aaactaacca taccatttac cataaaggta aaggcagcct gggaattgac
     7441 aactacacac tgtatatggc ggatcgcatt agctggcgta atgtgtcatt aatgcccggc
     7501 gtgcggtatg actatgacaa ctatctgtca aaccacaata tctccccgcg ctttatgacg
     7561 gaatgggata tttttgctga tcaaacctca atgattaccg caggttataa ccgttactat
     7621 ggtgggaata ttcttgatat gggattacgt gatatccgca atagctggac ggaatcggta
     7681 tcaggcaata aaaccctgac gcgttatcag gatttgaaaa cgccttataa cgatgaactg
     7741 gcaatgggat tgcagcagaa aatcggtaag aacgttattg cgcgtgcaaa ctatgtttac
     7801 cgtgaagcgc atgatcaaat cagcaaaagc agtcgtaccg acagcgcgac taaaaccacc
     7861 attactgaat ataacaacga tggcaaaacc aaaacgcatt cgttcaacct cagttttgag
     7921 ctggccgaac ccctgcatat ccgccaggta gatattaacc cacaaattgt ctttagctat
     7981 atcaagagca agggcaactt gtcgttaaac aatggttatg aggagagcaa taccggtgat
     8041 aaccaggtgg tttataacgg taatcttgtc tcttacgata gcgttccagt ggcagatttt
     8101 aataacccat taaagatctc cttaaacatg gatttcacgc atcaaccgag cgggttggtg
     8161 tgggcgaata cgctggcctg gcaagaagcg cgtaaagctc gcattatcct gggtaagaca
     8221 aatgcgcaat acatcagcga atattcagat tacaagcagt atgttgacga aaaactggat
     8281 agcagcctga cctgggacac ccgcttgtcc tggacgccac aatttctgaa acaacaaaac
     8341 ctgacgatca gtgccgatat tctcaatgta ttggatagca aaaccgctgt tgatacaacg
     8401 aataccggtg tggcgaccta cgccagtggc cgtactttct ggcttgatgt cagcatgaaa
     8461 ttttaatagt tcggatttta cttaaccggg taaattcgcc cggttttcgc atggagataa
     8521 ttatgagaaa cctctgtttc ttactgacgt tagtggcaac tctgttgctc cccgggcggc
     8581 tgattgccgc cgccttaccg caggatgaaa agttaattac cgggcaactg ggcaatggct
     8641 tgcgatatat gatttatccg catgctcatc caaaggatca ggtaaattta tggctgcaaa
     8701 ttcataccgg ttcattgcag gaagaagaca atgagcgcgg cgtggctcat tttgtagaac
     8761 atatgatgtt taacggcaca aaaacatggc cgggtaataa agtcatcgaa acatttgagt
     8821 caatgggcct gcgttttggt cgcgatgtta atgcctatac cagctatgac gaaacggtgt
     8881 atcaggtgag tttgccgact acgcagaaac aaaatctgca acaagtgatg gcaatcttca
     8941 gtgaatggag taatgccgca acctttgaaa aactcgaagt agacgctgaa cgtggcgtaa
     9001 ttactgagga atggcgtgcc catcaggatg cgaaatggcg cacctctcag gcgcgccgcc
     9061 ctttcctgct ggctaatacc cgtaatttag accgtgaacc tatcggcctg atggatactg
     9121 tcgccacggt cacaccggca caattgcgcc aattttatca acgctggtat caaccaaata
     9181 atatgacctt tatcgtggtc ggcgatatcg acagtaaaga agcgctggcg ctgataaagg
     9241 ataatttaag taagcttccg gctaacaaag cagctgaaaa tcgcgtctgg ccgacaaaag
     9301 ccgaaaacca cctgcgcttt aatatcatta atgataaaga aaaccgggtg aacggcatcg
     9361 cactctatta tcgcctgcca atggtacaag tgaacgatga gcaaagcttt atcgaacaag
     9421 ctgaatggag catgttagtt cagctgttca atcaacgtct gcaggaacgc atacagtcgg
     9481 gcgagttgaa gactatttct ggcggcactg cgcgcagcgt taaaattgca cccgattatc
     9541 agtcgctgtt tttccgtgta aatgcacgag acgataatat gcaggatgct gcgaatgcat
     9601 taatggcaga gttggcaacc attgatcagc atggtttttc tgctgaagaa ctcgatgatg
     9661 tcaaatctac ccgcctcacc tggctgaaaa atgcggttga tcagcaagct gaacgtgatt
     9721 tacgtatgct gaccagtcgc ctggcatcca gctcattaaa taatacgccg ttcttgtcgc
     9781 cggaagagac atatcaactt tcgaaacgtc tgtggcagca aattaccgtg caaagtctgg
     9841 cggaaaaatg gcagcagtta agaaagaacc aggacgcatt ttgggagcaa atggtaaaca
     9901 atgagcttgc cgccaaaaaa gcattgtctc ctgcggctat cctggcgctg gaaaaagagt
     9961 acgccaacaa aaagctggcg gcttacatct tcccaggcag aaatttatcg ttaacagtag
    10021 acgctgaccc acaggcggaa attagcagca aagaaacgct ggctgagaat ctgacatcat
    10081 taacactttc caatggtgcc agggttattc tggcaaaatc cgcgggtgaa gagcaaaagc
    10141 tacaaattac tgccgtatcg aataaaggcg atttaagttt ccctgcgcag caaaaatcac
    10201 ttatcgcgct ggcaaataaa gcagtaagcg gaagcggcgt tggtgagctc tcctcttcca
    10261 gcctgaaacg ctggagtgcg gaaaattcgg taaccatgag cagtaaagtc agtggcatga
    10321 atacgttgct ctccgttagc gcgcggacta ataaccctga acctggtttt cagttgatta
    10381 accagcgaat cacccacagc acgattaacg acaatatttg ggcatcgcta caaaatgctc
    10441 aaattcaggc gttgaaaacg ctcgaccagc gtccagcgga gaaattcgcc cagcagatgt
    10501 atgagacgcg ctatgctgat gaccgcacga aattactgca agaaaatcag attgtacagt
    10561 ttactgccgc agatgcgctg gctgccgatc gccagttgtt ttcatctcca gcggatatca
    10621 cgtttgtcat tgtcggtaat gtctcagaag acaaactcgt ggcgttaatt acgcgttact
    10681 taggatcaat caaacactct gattcgccat tagccgcagg taaaccatta actcgcgcga
    10741 cggacaacgc atcggttact gtaaaagaac aaaatgaacc tgtggcacag gtttcacagt
    10801 ggaagcgtta tgattcccgg acacctgtta atctggcgac gcgtatggcg ctcgatgctt
    10861 ttaacgtcgc actggcaaaa gatctacgtg ttaatattcg tgaacaggca tctggagcat
    10921 acagcgtttc ttctcgcctc tcggttgatc ctcaggccaa agatatcagt catttgctgg
    10981 cttttacttg tcaaccagaa cgacatgatg aactgttaac gttagcgaat gaagtgatgg
    11041 ttaagcgtct ggctaaaggg atcagtgagc aagaactgaa tgaataccag caaaacgttc
    11101 agcgcagcct cgatatccaa cagcgtagcg ttcaacaatt agcgaacact attgtaaata
    11161 gtcttattca atatgacgat cctgcagcat ggactgagca ggagcaattg ttgaaacaaa
    11221 tgacggtaga gaatgttaac actgccgtta aacaatatct ttctcatccg gtaaatactt
    11281 ataccggagt attattgcca aaataataac agccccgtca acacatcgtt ggcgggga
//
LOCUS       DAZFQD010000159        10996 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.159, whole genome shotgun sequence.
ACCESSION   DAZFQD010000159 DAZFQD010000000
VERSION     DAZFQD010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10996
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.159"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..82)
                     /locus_tag="ACHKTW_004399"
     CDS             complement(<1..82)
                     /locus_tag="ACHKTW_004399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012304878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6465308.1"
                     /translation="MIYITLPFIQLVYFISSEKKLTIHIVQ"
     gene            complement(171..788)
                     /gene="yncG"
                     /locus_tag="ACHKTW_004400"
     CDS             complement(171..788)
                     /gene="yncG"
                     /locus_tag="ACHKTW_004400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HFO6465309.1"
                     /translation="MIKVYGVPGWGSTISELMLTLADIPYQFVDVSGFDHEGTSRELL
                     KTLNPLCQIPTLALENDEIMTETAAIALMVLDRRPDLAPPVGRAERQQFQRLLVWLVA
                     NVYPTFTFADYPERWAPDAPEQLKKNVIEYRKSLYIWLNSQLTAEPYAFGEQLTLVDC
                     YLCTMRTWGPGHEWFQDNATNISAIADAVCQLPKLQEVLKRNEII"
     gene            1055..2554
                     /gene="ansP"
                     /locus_tag="ACHKTW_004401"
     CDS             1055..2554
                     /gene="ansP"
                     /locus_tag="ACHKTW_004401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparagine permease"
                     /protein_id="HFO6465310.1"
                     /translation="MSKHDTDTSDQHAAKRRWLNAHEEGYHKAMGNRQVQMIAIGGAI
                     GTGLFLGAGARLQMAGPALALVYLICGLFSFFILRALGELVLHRPSSGSFVSYAREFL
                     GEKAAYVAGWMYFINWAMTGIVDITAVALYMHYWGAFGGVPQWVFALAALTIVGTMNM
                     IGVKWFAEMEFWFALIKVLAIVIFLVVGTVFLGSGQPLDGNTTGFHLITDNGGFFPHG
                     LLPALVLIQGVVFAFASIEMVGTAAGECKDPQTMVPKAINSVIWRIGLFYVGSVVLLV
                     MLLPWSAYQAGQSPFVTFFSKLGVPYIGSIMNIVVLTAALSSLNSGLYCTGRILRSMA
                     MGGSAPSFMAKMSRQHVPYAGILATLVVYVVGVFLNYLVPSRVFEIVLNFASLGIIAS
                     WAFIIVCQMRLRKAIKQGKAADVSFKLPGAPFTSWLTLLFLLSVLVLMAFDYPNGTYT
                     IAALPIIGILLVIGWFGVRKRVAEIHSTAPVVEEDEEKQEIVFKPETAS"
     gene            complement(2669..3730)
                     /gene="yncE"
                     /locus_tag="ACHKTW_004402"
     CDS             complement(2669..3730)
                     /gene="yncE"
                     /locus_tag="ACHKTW_004402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HFO6465311.1"
                     /translation="MHLRHLFSSRLRGSLLLGSLLVASSFSTQAAEEMLRKAVGKGAY
                     EMAYSQQENALWLATSQSRKLDKGGVVYRLDPVTLEVTQAIHNDLKPFGATINNTTQT
                     LWFGNTVNSAVTAIDAKTGEVKGRLVLDDRKRTEEVRPLQPRELVADDATNTVYISGI
                     GKESVIWVVDGENIKLKTAIQNTGKMSTGLALDSKGKRLYTTNADGELITIDTADNKI
                     LSRKKLLDDGKEHFFINISLDTARQRAFITDSKAAEVLVVDTRNGNILAKVAAPESLA
                     VLFNPARNEAYVTHRQAGKVSVIDAKSYKVVKTFDTPTHPNSLALSADGKTLYVSVKQ
                     KSTKQQEATQPDDVIRIAL"
     gene            3972..6074
                     /gene="pqqU"
                     /locus_tag="ACHKTW_004403"
     CDS             3972..6074
                     /gene="pqqU"
                     /locus_tag="ACHKTW_004403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor PqqU"
                     /protein_id="HFO6465312.1"
                     /translation="MKIVFVRQTVLPALLVLSPVVFAADEQTMIVSAAPQVVSELDTP
                     AAVSVVDGEEMRLATPRINLSESLTSVPGLQVQNRQNYAQDLQLSIRGFGSRSTYGIR
                     GIRLYVDGIPATMPDGQGQTSNIDLSSVQNVEVLRGPFSALYGNASGGVMNVTTQTGQ
                     QPPTIEASSYYGSFGSWRYGLKATGATGDGTQPGDVDYTVSTTRFTTHGYRDHSGAQK
                     NLANAKLGVRIDEASKLSLIFNSVDIKADDPGGLTKAEWKANPQQAPRAEQYDTRKTI
                     KQTQAGLRYERSLSSRDDMSVMMYAGERETTQYQSIPMAPQLNPSHAGGVISLQRHYQ
                     GIDSRWTHRGELGVPVTFTTGLNYENMSENRKGYNNFRLNSGMPEYGQKGELRRDERN
                     LMWNIDPYLQTQWQLSEKLSLDAGVRYSSVWFDSNDHYVTPGNGDDSGDASYHKWLPA
                     GSLKYAMTDAWNIYLAAGRGFETPTINELSYRADGQSGMNLGLKPSTNDTIEIGSKTR
                     IGDGLLSLALFQTDTDDEIVVDSSSGGRTTYKNAGKTRRQGAELAWDQRFAGDFRVNA
                     SWTWLDATYRSNVCNEQDCNGNRMPGIARNMGFASIGYVPEDGWYAGTEARYMGDIMA
                     DDENTAKAPSYTLVGLFTGYKYNYHNLTVDLFGRVDNLFDKQYVGSVIVNESNGRYYE
                     PSPGRNYGVGMNIAWRFE"
     gene            complement(6110..6775)
                     /gene="mcbR"
                     /locus_tag="ACHKTW_004404"
     CDS             complement(6110..6775)
                     /gene="mcbR"
                     /locus_tag="ACHKTW_004404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HFO6465313.1"
                     /translation="MPGMGKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQL
                     GMSITPVREALLRLVSVNALSVAPAQAFTVPEVGKRQLDEINRIRYELELMAVALAVE
                     NLTPQDLAELQELLEKLQQAQEKGDMEQIINVNRLFRLAIYHRSNMPILCEMIEQLWV
                     RMGPGLHYLYEAINPAELREHIENYHLLLAALKAKDKEGCRHCLAEIMQQNIAILYQQ
                     YNR"
     gene            complement(6973..8010)
                     /gene="curA"
                     /locus_tag="ACHKTW_004405"
     CDS             complement(6973..8010)
                     /gene="curA"
                     /locus_tag="ACHKTW_004405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HFO6465314.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVVESNHPDYQPGDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATG
                     PVGATVGQIGKLKGCRVVGVAGGAEKCRHAIEVLGFDVCLDHHADDFAEQLVKACPKG
                     IDIYYENVGGKVFDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKKR
                     IRLQGFIIAQDYGHRIHEFQKEMGQWVKEDKIHYHEDITDGLENAPQTFIGLLKGKNF
                     GKVVIRVAGDD"
     gene            8191..8709
                     /gene="mddA"
                     /locus_tag="ACHKTW_004406"
     CDS             8191..8709
                     /gene="mddA"
                     /locus_tag="ACHKTW_004406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HFO6465315.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHQSLGFVVTAQMPQVGTKFGRWLDLTFMQL
                     QLDERTEPDAIG"
     gene            8706..9155
                     /gene="ydcZ"
                     /locus_tag="ACHKTW_004407"
     CDS             8706..9155
                     /gene="ydcZ"
                     /locus_tag="ACHKTW_004407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFO6465316.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMVRITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLMLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(9156..9389)
                     /gene="ydcY"
                     /locus_tag="ACHKTW_004408"
     CDS             complement(9156..9389)
                     /gene="ydcY"
                     /locus_tag="ACHKTW_004408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2526 family protein YdcY"
                     /protein_id="HFO6465317.1"
                     /translation="MSHLDEVIARVHAAIEESVIAHMNELLIALSDDAELSREDRYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(9475..9648)
                     /gene="ortT"
                     /locus_tag="ACHKTW_004409"
     CDS             complement(9475..9648)
                     /gene="ortT"
                     /locus_tag="ACHKTW_004409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HFO6465318.1"
                     /translation="MSLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            9843..9938
                     /gene="yncL"
                     /locus_tag="ACHKTW_004410"
     CDS             9843..9938
                     /gene="yncL"
                     /locus_tag="ACHKTW_004410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HFO6465319.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
     gene            complement(10340..>10996)
                     /locus_tag="ACHKTW_004411"
     CDS             complement(10340..>10996)
                     /locus_tag="ACHKTW_004411"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HFO6465320.1"
                     /translation="SKGDKITIEDNVIAEEYSTMPVKNFSSVGAFSFPTCHFSGNIRI
                     GRFCSIASNVKIMGGNHPLNRFTTHMMTYNGEFDKFAMSEFERSWTLKPFITKPENPI
                     IGNDVWIGNDVVLKGGIAIGDGAVIAANSVVTKDVPPYAIVAGVPAKIIRFRFDSNVI
                     DELLRIKWWNYNYSDLPDNNKCDDINYFVEEMNRLISDGNIQERDCKKFNLSEVFRGL
                     "
BASE COUNT         2748 a         2636 c         2592 g         3020 t
ORIGIN      
        1 tctgcacaat gtgaatagtt agtttctttt cagaggaaat gaaatagaca agctgaatga
       61 aggggagagt gatataaatc aaaaaaacat aacatgaatt taattaatta cgcccatcat
      121 tgtggataaa gttactgaat aataaataac ataatcctct ggtatcccac tcaaataatt
      181 tcattccttt ttaatacttc ttgtaatttc ggtaactgac atacagcatc agcaatcgca
      241 ctgatattcg tggcattatc ctgaaaccat tcatgcccag gcccccatgt gcgcatagtg
      301 caaagataac agtccaccag cgttagttgc tcaccaaacg cataaggttc agcggtcaat
      361 tgtgaattca gccagatata aagcgattta cgatattcaa ttacattctt ctttaactgt
      421 tcaggtgcgt caggagccca acgctcaggg taatcggcga aagtgaatgt tggatagaca
      481 ttagcaacca gccagaccaa tagccgttga aactgctgac gttcggcgcg cccaaccggt
      541 ggcgcaaggt ccgggcgacg atcgagaacc atcaatgcaa tcgccgctgt ctccgtcata
      601 atctcgtcat tttccagcgc cagtgtcgga atctgacaca acgggttcag ggttttcaat
      661 aactcgcgtg aagttccctc gtggtcaaaa ccactgacat caacgaactg ataaggaata
      721 tcagccagtg ttagcatcag ctcactgatt gtagagcccc atccgggtac gccatacacc
      781 ttaatcatat gcccctcaca atcttttaaa gattaagtgt aggacagtac acaggttaac
      841 cagcaaaagg atttgtcatt ttttcgtcag ttgtattaat ccccttcagg attgtaaggg
      901 gatgttttgc atcgtttatc gataacgggt aggatgctgc gccgatagtt ttatacccat
      961 aaagaataat ggtgataact atcatcgcca ggatgaataa acattgttca tggcaactta
     1021 tatgactttt tcattaaagc aatcagggag agcaatgagt aaacacgaca ccgacacttc
     1081 agatcaacac gccgcgaaac gccgctggct taatgcccac gaagaggggt atcacaaagc
     1141 gatgggcaat cgccaggtac agatgatcgc cattggcggc gcgattggca ccggcttgtt
     1201 tttaggtgca ggagcccgac tgcaaatggc gggtccagca ctggcactgg tttatttaat
     1261 ttgtggcttg ttttcgtttt ttattctgcg tgcattgggt gagctggtgc tacaccgccc
     1321 ttccagtggc agttttgtct cttatgcccg tgagtttttg ggtgagaaag ccgcttatgt
     1381 tgctggctgg atgtacttca tcaactgggc gatgacgggg attgttgata ttaccgccgt
     1441 cgctctgtat atgcattact ggggtgcgtt tggcggcgtg ccgcagtggg tctttgcgct
     1501 cgctgcactt accatcgttg gcaccatgaa tatgatcggt gtgaaatggt ttgcggagat
     1561 ggagttctgg tttgcgctta ttaaagtgct cgccattgtg atctttttgg tcgtgggtac
     1621 agtgttcctc ggtagtggtc agccgctgga tggcaacacc actggctttc atttaatcac
     1681 cgataatggc ggcttcttcc cccacggttt gctgcctgcg ctggtgttga ttcagggcgt
     1741 agtgtttgct tttgcctcca ttgaaatggt gggtacagct gccggagaat gtaaagatcc
     1801 gcagaccatg gtgcctaaag ccattaacag tgtgatttgg cgtattggcc tgttttacgt
     1861 cggctccgtg gtgttgctgg ttatgctatt gccgtggagc gcgtatcagg cggggcaaag
     1921 tccgttcgtg acgtttttct ctaaactggg tgtgccatat atcggcagca ttatgaatat
     1981 tgtggtgctg accgctgccc tctccagcct gaactcaggt ctgtactgca ccggacgtat
     2041 tctgcgctca atggcgatgg gcggttccgc accgagtttt atggcgaaaa tgagtcgtca
     2101 gcatgtgccg tatgccggga ttctggcgac actagttgtg tatgtcgtcg gcgtattcct
     2161 caactatctg gtgccgtcgc gcgtatttga gattgtgttg aacttcgcgt cgctgggaat
     2221 catcgcttca tgggcgttta tcatcgtgtg ccagatgcgc ctgcgtaaag caattaaaca
     2281 aggcaaagca gcggatgtca gttttaaact gcctggcgcg cccttcactt cctggctgac
     2341 attactgttt ttactgagtg tcctagtgct gatggcgttc gattacccga acgggactta
     2401 tactatcgcg gcgctgccga ttatcggtat tctgctggtt ataggctggt ttggtgtgcg
     2461 caaacgcgtt gctgaaattc acagcactgc gccagtcgtc gaggaagatg aagaaaaaca
     2521 ggaaattgtg tttaagcctg aaacggcgag ctaattgcaa aattgcccgg ctacgtgccg
     2581 ggcaaattta gattactgac gaaggtgtgc cgcatgcggc gtgataaaag tcaggcaagt
     2641 ctctccgttt tcaccctcgc cacatctatt acagcgcaat acgaatcaca tcgtccggct
     2701 gggtagcttc ctgctgttta gtggattttt gtttcacact gacatacagc gttttgccat
     2761 cggcagacag cgccaggctg tttggatgag tcggcgtatc gaacgttttc accactttat
     2821 agcttttcgc gtcaatcaca ctgactttac ctgcctgacg atgcgttacg taggcttcat
     2881 tacgcgcggg gttaaacagc acagccagtg attctggtgc cgcaaccttc gccagaatat
     2941 tgccattacg ggtatcgacc actaacactt ctgcggcttt agaatcggtg ataaatgcac
     3001 gctgcctggc ggtatcaagg ctaatgttga taaagaagtg ctctttgccg tcatccagca
     3061 gctttttacg gctgaggatt ttattgtcgg cggtgtcgat ggtaatcaat tcgccgtcag
     3121 cgttagtggt gtaaagacgt ttgcctttgc tatccagcgc cagaccggta ctcattttac
     3181 cggtgttctg gatggcggtt ttcagtttga tattctcgcc atcaacgacc caaatcacgc
     3241 tctctttacc aataccactg atgtaaacgg tgttcgtggc atcatcagct accagctcac
     3301 gcggttgcag cgggcgcacc tcttccgtgc gcttacgatc atccagcacc agacggcctt
     3361 tcacttcgcc agttttggca tctatcgccg tgaccgcgct gtttacggtg ttaccaaacc
     3421 acaacgtctg agtcgtgtta ttgatggtgg caccaaacgg cttgagatcg ttatggatcg
     3481 cttgggtcac ttccagagta accgggtcaa gacgataaac aacaccgcct ttatccagtt
     3541 tgcggctttg cgaagtggcg agccaaagcg cgttttcttg ctggctatat gccatttcgt
     3601 aggcaccttt acctaccgct ttacgcagca tttcttctgc ggcctgcgta ctgaatgatg
     3661 aagcaacaag caatgaacct aacagtaatg aaccacgcag acgcgatgaa aacagatgac
     3721 gtaaatgcat gacgactccc tttgatgaac cgatattttt gctgcttcac aatgcttcgc
     3781 taagcgtaat catcgttacg ctcttgttat aaatgggaat ggtaatcatt attttctcaa
     3841 atgtggagca tttcttgccg ttacgactac aattttctca attacttaac gtatcgtgcc
     3901 gtttcgtttt ccataaattt acaattttat taccatgacg gcgttttccc attcttaacc
     3961 tagctttttt catgaagatt gttttcgttc gacagaccgt tttgcccgca ctgcttgtcc
     4021 tttcccccgt tgtttttgcc gctgatgaac agaccatgat tgtcagtgcc gcgccgcagg
     4081 tggtttcaga actggatacc cctgcagcag taagcgtggt ggatggcgag gagatgcgcc
     4141 tggcaacacc gcgcattaac ttgtccgaat cactgaccag cgtgcctggt ttgcaggtac
     4201 aaaaccggca gaactatgcg caagatttac agctgtcgat tcgcggattt ggctcccgct
     4261 ccacttacgg tattcgcggt attcgcctgt atgtggacgg tattcccgcc accatgcccg
     4321 acgggcaagg gcaaacatcc aacatcgatt taagcagtgt gcaaaatgtg gaagtgctgc
     4381 gtggcccctt ttctgccctg tatggcaacg cgtctggcgg tgtaatgaat gtcaccaccc
     4441 agaccggaca acagccacca accattgaag ccagtagtta ctacggcagt tttggcagct
     4501 ggcgctatgg gctgaaagca acgggcgcaa cgggagacgg cacacaaccc ggcgatgttg
     4561 attacaccgt ctcaaccacg cgttttacga cccacggcta tcgtgaccat agtggcgcac
     4621 agaaaaattt agccaatgcc aaactgggcg tacgcattga tgaagccagc aaattaagcc
     4681 tgattttcaa tagtgtggat atcaaagcag atgacccagg tgggctaacc aaagcagaat
     4741 ggaaggcgaa tccacaacaa gcgcctcgtg cagaacagta cgacacgcga aaaaccatca
     4801 agcaaactca ggctgggttg cgctatgaac gtagcctgag ttcgcgggat gatatgagtg
     4861 tgatgatgta tgccggagag cgagaaacga cccagtacca gtcaataccc atggcaccac
     4921 aacttaaccc gtcacatgcg ggcggcgtga ttagcctgca acgccattac cagggaatag
     4981 acagccgctg gacacaccgt ggtgaactgg gcgttccggt cacgttcact accggcctga
     5041 actacgaaaa catgagtgaa aaccgcaagg gctacaataa cttccgcctg aatagcggca
     5101 tgccggagta cgggcaaaaa ggtgagttgc gtcgcgacga acgcaatctg atgtggaaca
     5161 tcgatcccta tttacagacg cagtggcagc tgagcgaaaa actgtcgctg gatgctggcg
     5221 tgcgctacag ctccgtgtgg tttgattcca acgaccatta cgttactccg ggtaacggcg
     5281 atgacagcgg tgatgccagt tatcataaat ggctacctgc cggttcgtta aaatatgcaa
     5341 tgaccgatgc ctggaatatc tatctggcag ccgggcgagg ttttgaaacg ccgacgatta
     5401 atgagctgtc ttatcgtgct gatgggcaaa gcggtatgaa cttaggttta aaaccatcca
     5461 ccaacgatac aattgagatc ggcagtaaaa cgcgtattgg tgatgggctg cttagtctcg
     5521 cattgtttca gaccgacact gatgatgaaa ttgttgtcga tagcagtagc ggtgggcgta
     5581 cgacttacaa aaatgccgga aagacccgtc gtcaaggcgc tgaactggca tgggatcaac
     5641 gtttcgcagg agattttcgc gtaaacgcgt cctggacctg gcttgatgcg acctatcgca
     5701 gcaatgtttg caatgaacag gattgtaacg gtaatcggat gccagggatc gcccgtaata
     5761 tgggctttgc gtcgataggt tatgtaccgg aagatggttg gtatgcaggc acggaagcgc
     5821 gttatatggg cgatattatg gcagatgatg aaaatacggc aaaagcgccg tcttatactc
     5881 tcgtcggctt attcaccggg tataaataca attaccacaa tttaactgtg gatttatttg
     5941 gtcgtgtcga taatttattc gataaacaat acgttggttc tgtcattgtc aatgagtcaa
     6001 acgggcgata ttacgaacct tcgcccggac gaaattatgg tgtcggcatg aatattgcgt
     6061 ggagatttga gtaattacct tgatgcccgg tatttgccgg gcatttactt taacgattgt
     6121 attgctggta taaaatagca atattttgtt gcataatttc agcaagacaa tgtctgcatc
     6181 cctctttgtc ttttgctttt aacgcagcga gtaacagatg atagttttct atatgctccc
     6241 gtaattccgc tggattaatc gcttcataga gataatgtaa accaggcccc atcctgaccc
     6301 acagttgctc aatcatctca cacaggatgg gcatatttga gcgatgataa atcgctaagc
     6361 gaaatagcct gtttacatta atgatttgtt ccatgtcgcc cttttcttgc gcctgctgta
     6421 atttctcgag caattcctgg agttccgcaa ggtcttgcgg ggtgaggttt tcaacagcca
     6481 gagcaactgc cattaattcc agttcgtagc ggatccgatt gatttcgtct aattgacgtt
     6541 tccccacttc cggaactgta aatgcttgtg caggtgcgac agaaagcgca ttcaccgaaa
     6601 ccagacgtaa taatgcttca cgcacaggtg taatactcat acctaattgc tccgccagat
     6661 ttttagtaat caggcgtgcg ccaggtttta atgcgccaat actaagctga tgtttcaggt
     6721 cgttctcaac ctgcagagtc aaactgacat gtttcatttt tcccattcca ggcattttaa
     6781 ttccttcgcg tgttttctgt ccaaccctgt ctgcacggca gaaggagttc acctggtgta
     6841 ttaaagtgaa taatcataaa ccacccacct ttgttgttat tagcagcaga acaatgacgt
     6901 ggttagttaa ttaaagctga aaataagctt tttgcctgga tttttatggc ggcttacgcc
     6961 gccgttgaaa ctttaatcat cacccgcgac gcggatcacc actttgccga agtttttacc
     7021 cttcagcagg ccgataaacg tctgtggcgc attctctaaa ccatcagtaa tatcttcgtg
     7081 gtagtggatt ttatcctctt tcacccattg ccccatctcc ttctgaaact catggatgcg
     7141 gtgaccataa tcctgagcga taataaaccc ttgcaagcga atacgttttt tcagcaccgt
     7201 tgccatcaac aggggtaaac gatccggacc gggtggtagc tctgtagcgt tatagctgct
     7261 cactaagcca caaacgggaa tgcgcgcaga tgtattaagt aatggtagca ctgcatcaaa
     7321 taccttaccg cccacgtttt cataatagat atcaatacct tttgggcacg ctttcaccag
     7381 ttgttcggca aaatcatccg cgtggtgatc aagacaaaca tcgaagccca gcacctcgat
     7441 agcatggcgg catttttccg cgccaccggc aacccccacc accctgcaac ctttaagttt
     7501 gccgatttgc cccaccgttg ctcccacagg acctgtcgcc gcagctacca ccaacgtttc
     7561 gccctcttta ggctgaccga tatccagcag ccccatataa gcggtaaagc ctggcatccc
     7621 cagcacaccc agcgaccacg atggattttg cggatgatcg ccaagtttca ccagatcatc
     7681 accactggat atgtcatagt cctgccatcc actgtagccc agcacccagt cgccaggctg
     7741 ataatcagga tgattcgact ccacaacacg gctcaccgtg ccgccgacca tcacgccgcc
     7801 aatatcaaca ggcggtgaat aagatggctc atcgctcata cgtccacgca tatacgggtc
     7861 cagggacaaa taaactgtgc gcagtaacac ctgtccttca cccggtgtgg cgacatcatc
     7921 ttcttcaaga cggaaattct ccggaaccgg tgcgccatgt ggacgcgagg ccagaaccca
     7981 acgtcgatta cgctgctttt gttgccccat aatgctctcc tgtgcttttg gcatgaatcc
     8041 atcaatagta gcaggctcac gggattggtt ttgtttgcag ccgtcacatc ccctctgcaa
     8101 cacgcaataa tttcatcgag gaatccgttg cttcgggctg ttttcgatag tattgataac
     8161 atcatcatca ttattttatc gaggccagac atgtccatcc gttttgcccg caaagccgat
     8221 tgtgctgcca ttgcggaaat ttataaccac gccgtgttgt atacggcggc tatctggaat
     8281 gaccaaacgg ttgatgcaga taaccgcatt gcctggtttg aagcgcggac catagcaggt
     8341 tatccggtgc tggtgagtga agaagatggc gtagtgacgg gatatgcctc gtttggcgac
     8401 tggcgtagtt tcgatggttt tcgccatacc gtggaacatt cggtttatgt ccatcccgat
     8461 catcagggca aaggtctggg gcgtaaattg ttaagccgat tgattgatga agcgcgggat
     8521 tgcgggaagc atgtcatggt cgccgggatc gaatcgcaaa atcaggcctc gctgcatctg
     8581 caccagtcgc tgggatttgt cgtcaccgca caaatgccgc aggtgggcac taaatttggg
     8641 cgctggctgg atctgacatt tatgcagttg caactcgacg aacgcactga accggacgcg
     8701 attggatgaa tcagtcgctc acccttgctt ttttgattgc cgccggaatt ggtctggtag
     8761 tccagaacac gctgatggtg cgcatcaccc agacctcctc taccattctc atcgccatgt
     8821 tgctgaactc actggtgggg attgtgctct ttgtttctat tttatggttt aaacaaggca
     8881 tggcggggtt tggcgaactg gtgtccagcg tgcgctggtg gacacttata cccggcttgc
     8941 tgggatcgtt ttttgtcttc gccagtatca gcggatatca gaatgtggga gcggcgacaa
     9001 ctattgcagt actggtcgcc agccagctaa ttggcggtct gatgctggat atcttccgca
     9061 gtcatggcgt accactacgg gcgttgttcg gcccaatttg tggcgcaatt ttacttgtgg
     9121 ttggcgcctg gttggtagcc agacgctcat tttaatcaga ggatggtgcc gcctttggtt
     9181 aactgttcgt ggcgcgcttc catatcttct ttatgcttgc gaccgtgatg agcaatagcg
     9241 gtgcgcaaac gctgctgctg ggtgtagcga tcttcccgac ttaactccgc gtcatcgctc
     9301 agggcgatta ataattcgtt catatgggca atgacgctct cttcaatcgc ggcatgcacg
     9361 cgcgcgatga cttcatccag atgcgacata ctttctcctt aaccacttgt gaaatttatc
     9421 gattcatcca aatgatataa gtctgcgtgg tgccgtcaag ccagtgagta agatttaaaa
     9481 cagtgaaaac aacagcgcca ccgggaaact catcggccat gttgccccca ccagaaaggc
     9541 gcttaagaaa cggatgcgtt tcttatcgtg agaaagaaac caggtgataa gaaatgcgat
     9601 tgctgccata accgcataaa aaacaagcat atgttgatag agagacatgt acgggcgtcc
     9661 agccagatga ataaaacgcg cgcaaatatg cgtcattttt tgacgtgcat caagtttttt
     9721 caccagtttg agaaaaaatc atcaaacagc aacttgccct tatccatctt cagaatgagt
     9781 attattgcgg attttcttaa cccgtactat acacataggg gcaatgataa aaggtggcaa
     9841 aaatgaatgt ttccagtaga actgtagtac tgataaatgt ctttgctgct gttggtttgt
     9901 ttactcttat ctctatgaga tttggctggt ttatttgatg tagtgatttt gcacgcagca
     9961 cgtactgtca gagaaatcaa ctctctgaca gtacgcaaga tcttctcata ctctccaggt
    10021 ttacaacatt atccttaata tttacttctt tttgctggca tctacattga aatgagtaga
    10081 aaacatcccc ctactgtcgt ctatacaaac agcttactga ttcaaacgga atcttgtttt
    10141 cacatttttc tatcatgaat actttatacg cgcgggagtc tataaatgct ttatactttc
    10201 aaggaataac gtaaatataa aaagatggcg taaagtatat gcatggcagg atgtttgcaa
    10261 cctccttgat gacgctgccc cattgaaatt gcaaaccccg ccctggttat cttgcccatt
    10321 tatcatgcaa aacagataat tataggcctc tgaatacctc agaaagattg aattttttac
    10381 agtccctctc ttgaatattt ccatctgata tcaatcgatt catctcttca acaaaataat
    10441 ttatatcatc acacttgtta ttatccggaa gatctgagta gttataattc caccatttta
    10501 ttctcaacag ttcatcaata acatttgaat caaacctaaa tctgattatt tttgcaggaa
    10561 ctcctgcaac tatggcatat gggggcacat ctttggtaac aacggaattg gcagcaataa
    10621 cagcaccatc acctattgcg atgccacctt tcaatacaac atcatttcct atccagacat
    10681 catttcctat aatcggattt tcaggtttgg taatgaatgg tttcaatgtc cagctacgtt
    10741 caaattcact catagcaaac ttatcaaatt caccgttata agtcatcatg tgagtagtaa
    10801 aacgattcaa aggatgattt ccacccatta tttttacatt tgatgcaata ctacaaaatc
    10861 gacctatacg tatattccct gaaaaatggc aggtcggaaa tgaaaaagcg ccgacactgg
    10921 agaaattttt cacaggcatt gttgaatatt cttcagcaat aacattatct tcaattgtta
    10981 tcttgtcacc ttttga
//
LOCUS       DAZFQD010000160        10974 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.160, whole genome shotgun sequence.
ACCESSION   DAZFQD010000160 DAZFQD010000000
VERSION     DAZFQD010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10974
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.160"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(36..1865)
                     /gene="glmS"
                     /locus_tag="ACHKTW_004412"
     CDS             complement(36..1865)
                     /gene="glmS"
                     /locus_tag="ACHKTW_004412"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFO6465321.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEVNAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            complement(2027..3397)
                     /gene="glmU"
                     /locus_tag="ACHKTW_004413"
     CDS             complement(2027..3397)
                     /gene="glmU"
                     /locus_tag="ACHKTW_004413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFO6465322.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGSKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            complement(3748..4167)
                     /locus_tag="ACHKTW_004414"
     CDS             complement(3748..4167)
                     /locus_tag="ACHKTW_004414"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFO6465323.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            complement(4188..5570)
                     /gene="atpD"
                     /locus_tag="ACHKTW_004415"
     CDS             complement(4188..5570)
                     /gene="atpD"
                     /locus_tag="ACHKTW_004415"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFO6465324.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            complement(5597..6460)
                     /gene="atpG"
                     /locus_tag="ACHKTW_004416"
     CDS             complement(5597..6460)
                     /gene="atpG"
                     /locus_tag="ACHKTW_004416"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFO6465325.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            complement(6511..8052)
                     /gene="atpA"
                     /locus_tag="ACHKTW_004417"
     CDS             complement(6511..8052)
                     /gene="atpA"
                     /locus_tag="ACHKTW_004417"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFO6465326.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            complement(8065..8598)
                     /gene="atpH"
                     /locus_tag="ACHKTW_004418"
     CDS             complement(8065..8598)
                     /gene="atpH"
                     /locus_tag="ACHKTW_004418"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFO6465327.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLAFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            complement(8613..9083)
                     /gene="atpF"
                     /locus_tag="ACHKTW_004419"
     CDS             complement(8613..9083)
                     /gene="atpF"
                     /locus_tag="ACHKTW_004419"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFO6465328.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            complement(9145..9384)
                     /gene="atpE"
                     /locus_tag="ACHKTW_004420"
     CDS             complement(9145..9384)
                     /gene="atpE"
                     /locus_tag="ACHKTW_004420"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HFO6465329.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            complement(9431..10246)
                     /gene="atpB"
                     /locus_tag="ACHKTW_004421"
     CDS             complement(9431..10246)
                     /gene="atpB"
                     /locus_tag="ACHKTW_004421"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFO6465330.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQHPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYSIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            complement(10255..10635)
                     /gene="atpI"
                     /locus_tag="ACHKTW_004422"
     CDS             complement(10255..10635)
                     /gene="atpI"
                     /locus_tag="ACHKTW_004422"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HFO6465331.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
BASE COUNT         2606 a         3003 c         2725 g         2640 t
ORIGIN      
        1 accggtcggc agcccttttt tatctatgat agtgattact caaccgtaac cgattttgcc
       61 aggttacgcg gctggtcaac gtcggtgcct ttgatcagcg cgacatggta agccagcagc
      121 tgcagcggaa cggtgtagaa gatcggtgca atcacctctt ccacatgcgg catctcgatg
      181 atgtgcatgt tatcgctact tacaaaaccc gcatcctgat cggcgaagac atacaactga
      241 ccgccacgcg cgcgaacttc ttcaatgttg gatttcagtt tttccaacag ttcgttgttc
      301 ggtgcaacga cgataaccgg catatccgcg tcaatcagcg ccagcgggcc gtgtttcagc
      361 tcgcctgcag cgtaggcttc agcgtgaatg taagagatct ctttcagttt caatgcgcct
      421 tccagcgcga ttgggtactg atcgccacgg cccaggaaca gcgcgtgatg tttgtcagag
      481 aaatcttctg ccagagcttc aatgcgtttg tcctgagaca gcatctgctc aatacggctc
      541 ggcagcgcct gcagaccatg cacgatgtca tgttcaatgg aggcatccag acctttcagg
      601 cgagacagct tcgccaccag catcaacagc acagttaact gagtggtgaa tgctttagtg
      661 gatgccacgc cgatttccgt acccgcgttg gtcatcagcg ccagatcgga ttcgcgcacc
      721 agagaagaac ccggaacgtt acagattgcc agcgaaccaa ggtaacccag ctctttcgac
      781 agacgcaggc ccgccagggt atccgcggtt tcgccagact gtgacaaggt tatcatcagg
      841 ctgttacgac gcacggcaga tttgcgatag cggaattcag aggcgatttc gacgtcgcac
      901 ggaatacctg ccagcgattc aaaccagtaa cgggaaacca taccggagtt ataagaagta
      961 ccacaggcga ggatctgaat atgctcaacc ttcgacagca gttcgtcggc gttcggtccc
     1021 agctcgctta aatcaacctg accgtggctg atgcgcccgg taagggtgtt ttttatcgcg
     1081 ttcggctgtt cgtagatctc tttctgcatg tagtgacggt aaataccttt atcgcccgcg
     1141 tcatattgca gattggattc gatatcctga cgttttactt ccgcgccagt tttatcgaag
     1201 atgtttaccg aacggcgagt aatttccgca atatcgccct cttcaaggaa gataaagcga
     1261 cgggtcaccg gcaacagcgc cagctggtca gaagcgataa agttttcgcc catacccagg
     1321 ccaatcacca gcggactacc agaacgtgcc gccagcaggg tatccgggtg acgggagtcc
     1381 atgatcactg taccgtacgc accacgcagt tgcgggatag cacgcagaac agcctcacgc
     1441 agagtaccgc cctgtttcag ctcccagttc accagatggg caattacttc ggtgtctgtt
     1501 tcagaaacga aggtatagcc acgcgctttt aactcttcgc gcagcggttc atggttttcg
     1561 atgatgccgt tatgcaccac cacaatgtgt tcagaaacat gcggatgcgc attcacttct
     1621 gaaggttcac cgtgggtcgc ccagcgagta tgagcaatac ccgtaccgcc atgcagagga
     1681 tgttcttccg ctgcctgagc cagcatctgg actttaccga ggcgacgcag gcgggtcata
     1741 tgaccttccg catcaacaac ggccagaccg gcagagtcat atccgcggta ttccagacga
     1801 cgtaaacctt caagaaggat ttctgctaca tcacgttgcg cgatcgcgcc aacaattcca
     1861 cacatagttt ttgattccga tttatatcgt tgtcggtcaa cctgtatgcc cgttttttcg
     1921 ggcgccccga gccttgtaga gagtggggtt atttttatag ttactgcttg tgggagggag
     1981 attatgttat ctcctcatcc catgtgaccg gattacccgg ccagaatcac tttttcttta
     2041 ccggacgacg ccagccttct ttctgagtct gcggcacacg gctgatagct aatgcatttt
     2101 cgccgacatt acgcgtcaca gttgtacccg cagcaatggt cgcgcctttg cctactgtta
     2161 ccggggccac cagctgagtg tcggaaccga caaacacatc gtcgccgata atggtcttaa
     2221 atttattcgc accatcgtag ttgcaggtaa tggttcccgc gccgatgtta acgttatcgc
     2281 caatttccgc atcgcccagg taagtcagat gaccagcttt cgagccttta cccagacgcg
     2341 cttttttcat ctcaacaaag ttaccgacgt gtgcaccttc cagcaactca gcaccaggac
     2401 gcagtcgggc aaacgggcca atagtacagg ccgctgccag attcgcgtct tccacaacgg
     2461 tatacggact gatttcgcaa tcatcgccaa tcacgctgtt tttaatcacg caaccggtgc
     2521 cgattttcac gcgatgaccg agagtcacgt tgccctcgat gataacgtta gtatcaattt
     2581 caacatcgcg cccgtgagta agcgtaccgc gcaaatcaaa acgcgccgga tcgcgcagca
     2641 taacgcctgc taacagcagt ttttcagcct gttcggactg gtaaacgcgc tccagacggg
     2701 agagttgcag gcggttattc acaccttcta cttcgcttaa acgttgcgga tgaacggcga
     2761 cgatttcacg cccttcctga tacgccagcg caataatgtc ggtgatgtag tattcgccct
     2821 gagcattatt gttggtcagc ttcgccagcc agcgtttcat atctgcgccg ttagcgatca
     2881 gaatgccggt gttgatctcc tgaatctgac gctgctcgtc ggtggcatct ttgtgctcaa
     2941 caatgccggt aactttgcca ttttcacggg tgatacgtcc ataaccggtc ggatcatcca
     3001 gtttcaccgt cagcagacca atgccaccct gcggtttagc atcacgcagg cgctggagtg
     3061 tttcgacaga gatcagcggc acgtcgccgt agagcattaa aatgtcttca tcatcggcaa
     3121 agaaaggtgc ggcctgctgc attgcatgac ccgtacccag ctgctctgcc tgaagcaccc
     3181 agttaaggtt gtcgtctttc agcgcctgtt taagcagatc gccgccgtga ccgtacacca
     3241 ggtgaacgtg cgctgcgcct aattcattcg cagcatcaat gacatgctga accatcgctt
     3301 tcccggcaag ggtatgcagc actttcggaa gatcggaata catgcgcgtg cctttgcctg
     3361 cggcaaggat cactacgctc atagcattat tcaacatacg cgtcctgact gtaatttgag
     3421 aacgaattta aaccgcttca ccttgaaaaa actacatttt tttcatcgtg aaatggacag
     3481 aggataaatt gttcaataac ggattatccc cgctgacgaa agtgccattt tcgaccatcg
     3541 tcacgccttt tgtctgcaga aaattaaggc agtaccttca tttaagcgtc aggtggatgt
     3601 ttttgctctt atttcgatca atgaataaac gggaaaacag gggtgtttta tatcagcagg
     3661 atctatgtga acgctcttca ggacggatca cgcgtaaaaa aaagccagcc tgtttccaga
     3721 ctggcttttg tgcttttcaa gccggtgtta catcgctttt ttggtcaact cgataacgcg
     3781 cagctgcgcg atcgctttgg ccagttccgc agacgcctga gcgtaatcta cgtcgccgtg
     3841 agagctgcta atgtgctctt cagccttacg tttcgcttcc atggctcgcg cttcgtcgag
     3901 atcctggccg cgaattgcgg tgtcggccag aacggtcacg ttgccaggct gcacttcaag
     3961 aatgccgcca gacagataga taaactcttc gtgaccgtgc tgtttcacga tgcgaatcat
     4021 accaggctta atggcggtga gcagcggtgc gtggccaggg tagatcccca gttcaccttc
     4081 gctacccgtt acctggattt tctcgaccag accagagaac atttgttgct ctgcgctgac
     4141 gacgtccagg tggtaagtca ttgccatatc accctccgat taaggcgtta aagttttttg
     4201 gctttttcca cagcttcttc gatggaaccg accatgtaga acgcctgctc cggcaggtga
     4261 tcgtattcgc cttccatgat gcctttaaag ccacggatgg tgtctttcag ggagacgtat
     4321 ttacccggag aaccggtgaa tacttctgcc acgaagaacg gctgggacag gaagcgctgg
     4381 atcttacgag cacgcgctac caccagtttg tcttcttcag acagttcatc catacccagg
     4441 atggcgatga tgtctttcag ttcctgataa cgttgcagga tggactgaac gccacgcgcg
     4501 gtgtcgtagt gttcctgacc aaccaccagc gggtccagct gacggctggt ggagtccagc
     4561 gggtcaacgg ccgggtagat acccagagac gcgatctgac ggctcagtac cacggttgcg
     4621 tcaaggtgcg caaaggtggt tgccggagac gggtcagtca agtcatccgc aggtacgtat
     4681 actgcctgta cggaggtgat agaaccagtt ttggtggagg tgatacgttc ctgcagaacg
     4741 cccatctctt ccgccagggt cggctgataa cctaccgctg aaggcatacg gcccagcagt
     4801 gcggatactt ccgtacctgc cagggtgtaa cgatagatgt tgtcaacgaa cagcagaacg
     4861 tcacgacctt cgtcacggaa tttctcagcc atggtcagac cggtcagagc aacgcgcaga
     4921 cggtttcccg gcggctcgtt catctggcca tacaccaggg atactttgtc gataacgttg
     4981 gagtcggtca tttcgtggta gaagtcgtta ccctcacgag tacgttcacc tacgcccgca
     5041 aacacagagt aaccggagtg ctcgatcgcg atgttacgaa tgagctccat catgtttacg
     5101 gttttaccta cacccgcacc accgaacaga ccaactttac cgcccttagc gaacggacac
     5161 atcaggtcga taactttgat accggtttcc agcagttcct gagagtttga cagctcttcg
     5221 taggaaggtg ctgcgcggtg aatcgcccaa cgctcttctt caccgatctc gcctttcatg
     5281 tcgaccggtt cacccagtac gttcatgata cggcccagag tcgctttacc taccgggact
     5341 tcgatcgggt gttcgaggtc ttttacatcc agaccgcgac gcagaccgtc ggaggaaccc
     5401 attgcgatgg tacgcacgat accgccgccg agctgctgct gaacttccag caccagacgc
     5461 tcattaccat tttgcacctc aagagcatcg tacacgcgcg gtacggcatc ctgagggaat
     5521 tcgacgtcaa ctacggcgcc gattacctgg acaatctttc cagtagccat cttaaatcct
     5581 ctacgaaata acctgtttaa accgcggcgg cccccgagac gatctcggtg agttcctgag
     5641 taatgctggc ctgacgagct ttgttgtata ccaactgcag ctctttaatc aggctgccgc
     5701 cattgtcggt cgcggctttc atcgccacca tacgggcggc ctgctcgctg gccaggtttt
     5761 caaccacgcc ctgataaacc tgagattcga cataacgacg cagcagggta tccagcaacg
     5821 ccttcggatc gggttcgtac aggtaatccc aggatttatg tttcagatca tcatcatctg
     5881 atgccggtaa cggcagcagc tggctgatgg tcggaacctg agacatggtg ttaataaatt
     5941 tgttgctgac aatgtaaagc ttgtccagac ggccttcgtc gtaggcctgc aacatcactt
     6001 ttaccggacc gatcagttcg gacagggaag ggttatcccc catgccggtg acctgggcaa
     6061 caacattgcc gcccacggag ttgaagaacg acacgccttt cgagccgatc attgcgaggt
     6121 cgcattgaac gcctttgtcg gtccaggtct tcatttccgc cagcagtttt ttgaacaggt
     6181 taatgttcaa accaccgcac aaaccacggt cggtcgacac caccaggtag cccacgcgtt
     6241 taacgtcgcg gtcttccagg taagggtgct tatattccag attaccgtgt gcaaggtgac
     6301 caatcacttt gcgcatggtt tctgcataag gacggctggc cgccatgcga tcctgcgatt
     6361 tacgcatttt ggaagcggcg accatctcca tcgctttagt gatcttttgc gtgttctgga
     6421 cgcttgcgat cttactacgt atctcttttg cgccggccat gagcttctcc tcaatgcctt
     6481 gcggcctgcc ctaaggcaag ccgccagacg ttaccaggat tgggttgctt tgaaggaatc
     6541 gaggatgcct ttcagcttgc cttcgatttc gtcgttgtag ccaccggtct ggttgatctc
     6601 ttgcatcaac ggagcgtgat cacggtcgac gtaagccagc agagcggctt cgaagctgcc
     6661 aattttcgac agttcaacat ccgccaggta accacgttct gctgcgaaca gaaccagaga
     6721 ctgctgcgca acggacatcg gcgcatactg tttctgtttc agcagttcgg tcactttctg
     6781 accgtggtca agctgcttac gtgttgcatc gtcaaggtcg gatgcaaact gagagaacgc
     6841 tgccagttca cgatactgtg ccagagcggt acggatacca ccggacagtt ttttcatgat
     6901 cttggtctgt gctgcaccac caacacggga tacggaaata cccgggttaa ccgcaggacg
     6961 aataccggcg ttgaacaggt tggtttccag gaagatctga ccatcggtaa tggagattac
     7021 gttggtcgga acgaacgcag aaacgtcacc cgcctgagtt tcgataatcg gcagtgcggt
     7081 cagagaaccg gttttccctt tcacttcacc tttggtgaag gcttcaacgt attcggcgtt
     7141 aacacgtgca gcacgctcca gcagacgaga gtggaggtag aaaacgtcgc ccgggaatgc
     7201 ttcacgtcct ggcggacgac ggagcagcag ggagatctga cggtaagcaa cagcctgttt
     7261 agacaggtca tcgtaaatga tcagcgcatc ttcaccgcgg tcacggaagt attcgcccat
     7321 tgcgcaaccg gcatacggtg ccaggtattg cagtgcagcg gattcagacg cggttgctac
     7381 cacaacgatg gtgttagcca gtgcgccgtg ctcttccagt ttacgtacca cgttagaaat
     7441 ggtggacgct ttctggccga tagcgacata gatacatttg ataccggaat cgcgctggtt
     7501 gatgatggca tcgatagcca gtgcggtttt acctgtctga cggtcaccga tgatcaattc
     7561 acgctgacca cgaccgattg ggatcatgga gtcaacggct ttataaccgg tctgtaccgg
     7621 ctgatctacg gactgacgtt cgataacgcc cggagcgatt gcttctacag cagagaagcc
     7681 gtcgtgatcc agcggacctt taccgtcgat tggtgcaccc agagtgttaa ccacacggcc
     7741 cagcaggcca cggccaaccg gaacttccag gatacggcca gtacacttaa ctttcatgcc
     7801 ttcggcaagg tcagcgtacg gacccataac aaccgcacct acagagtcgc gctcgaggtt
     7861 cagtgcgata gcgtaacggt tacccggcag ggagatcatt tcaccctgca tacaatcggc
     7921 caggccgtga atgcggataa caccgtcact tacagaaaca atagtacctt cgttgtgagc
     7981 ttcactcaca acattgaact gagcaatgcg ctgcttgatc agttcgctga tttcggtgga
     8041 attcagttgc atgctccagt ccccttaaga ctgcaagacg tctgcaaggc gctcaagacg
     8101 accgcgtacg ctgccatcaa tgaccatatc acccgctcgg atgataacgc ctgccattac
     8161 agacttatcg attttgcaat tcagcttaac tttgcgtgac agacgttttt ccatcgcagc
     8221 agaaattttc gcgagctgtt gttcactcag tgcggcagcg gaaatgacgt ctacctcagc
     8281 ggtagcctca ctcacggcac gaaggtgaat aaactgctcc agaacatccg ggagcgcgtt
     8341 aagacgacca ttttcagcca ttacccgaat caggttctga ccgttttcgt ccagttgctc
     8401 accacaaact gcgataaacg actcggcgag cgtttctggc gcaagcgcgc cagagagaag
     8461 ctctgccatt tgttcgtttt tggttacctc ggcggcaaac gccagcatgt cctgccagcg
     8521 ttctacactt tggtgttcga cggcaaagtc aaaagctgct ttggcgtagg ggcgagctac
     8581 cgtaataaat tcagacatca gcccctccct ccttacagtt cagcgacaag tttatccacg
     8641 atgtcgctgt tagcagcttc atccacggaa cgttcgatga tcttctcggc gccagcaaca
     8701 gccaggatag caacttgctt acgcagctct tcacgggcac gtttacgctc ggcttcaatt
     8761 tctgcctgcg cctgggccac gattttagta cgttcctgtt ctgcctcagc tttcgcttcg
     8821 tccagaatct gcgagcggcg tttattcgcc tgctcgatga ttacctgggc ttccgctttc
     8881 gcttttttca gctggtcggt cgcgctggcc tttgcaaggt caaggtcctt atgtgctcgt
     8941 tctgcggaag caaggccgtc agcaatttct ttttgacgtt tttcgatggc tgccattaat
     9001 ggcggccata cgtacttcat gcagaacaga acgaacagga caaacgcgat ggcctggccg
     9061 aggattgttg cgttaagatt cacagcacaa tgcctctatt tagttaacgt tctgatattg
     9121 ctctttaaat aaaagcaacg cttactacgc gacagcgaac atcacgtaca gacccagacc
     9181 tacagcgatc atcgggatag catccaccag acccataacg ataaagaact gagtacgcag
     9241 cagaggaatc agatcaggtt gacgcgctgc gccttccagg aatttacccc cgaggatgcc
     9301 gataccgatc gcagcaccga ttgccgccag acccatcatc acagcggcag ccatgtacag
     9361 cagatccata ttcaggtttt ccatgacagt ctccagtttg tttcagttaa aacgtagtag
     9421 tgttggtaaa ttaatgttct tcagacgcca tcgacagata gacgatcgtc agaaccatga
     9481 agatgaaggc ttgcagcgta atgatcagga tgtggaaaat ggcccacggc acattcagga
     9541 tccactgtga ccaccacggc aacagaccag caatcagaat gaaaatcagc tcaccggcat
     9601 acatgttacc gaacagtcgc aaaccaagtg aaactggttt ggacagcagg cttacccctt
     9661 caaggattaa gttgacagga atgaacgccc agtgattgaa cggctgcagc gtcaactctt
     9721 tcgtgaagcc gccgatgcct ttcattttga tgctgtagaa cagaatcagg ataaatacgc
     9781 ccaatgccat agacagcgtt acgttcacgt ccgcagacgg aaccacacgc agtgcaggca
     9841 gacccagtac atgttcagca atgtacggca gcaggtcgat aggcagtaaa tccatcaggt
     9901 tcatcaggaa tacccagacg aagatcgtca gggccagcgg agcaatcagc ttgcttttgc
     9961 catggtacat gtctttcacg ctaccattaa caaagccgat caccagctca atcgcggtct
    10021 gaaacttacc tggcacaccg ctggtcgcct ttttggctac gctacggaat aaaaccagga
    10081 acaacagacc cagcaccacc gagaagaaca tggagtcaat attgattgtc cagaaggtgg
    10141 ctggggggtg ttgtggatcc accagcgaga atgtacgcag gtccagctga aggttattca
    10201 ggtggtgtcc tatgtaatcc tgcggcgtca tattttctga agccatgatg ccttttaccc
    10261 tttgttgtta attacagccg gtgccagtat ctgaaccacc agcaccaaaa cccacgtaac
    10321 gatcagcggc aagaataccg cctttaaaac cgccaacgcc accaccagta acaccaacat
    10381 cgccagaact ttgaaagctt cgccaaatgc gaatgtccag gccacccggc ctttcgctgg
    10441 tgtatgcgcc tggtgacgcc aggcaaatat cataaacaaa acgttaggca gaaagactgc
    10501 caggcccccg cttattgcag agacgcccca gaaggggtct ttgaggctga acagcaatcc
    10561 acttgctatc accaccagta actgaacgag cagaagcttc cgagcaacgt ttcgactcac
    10621 gagcgacaca gacatcacgt ttttcactcc tgctcccttc gaggtatgcc gcgtgtcgta
    10681 taaaactttc tttaaggctt agagtcaagc atcaaaaagc ggtcaaatta tacggtgcgc
    10741 ccccgtgatt tcaaacaata agtagccaaa aggtgaataa atgtttaaat atttttccag
    10801 tgcatacaat tgcgactttt ctgctaaccc tgttcgatca tgaaaaactg taaataacgc
    10861 gtaaacactg gtgataaagc gtgcttcaga tcacatattg cgcatgttcg cgcacagcat
    10921 atttatttac ttggcaaatg atgcctttgc aagtttatga tatttcagtc taaa
//
LOCUS       DAZFQD010000161        10921 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.161, whole genome shotgun sequence.
ACCESSION   DAZFQD010000161 DAZFQD010000000
VERSION     DAZFQD010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10921
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.161"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..224
                     /locus_tag="ACHKTW_004423"
     CDS             <1..224
                     /locus_tag="ACHKTW_004423"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6465332.1"
                     /translation="NPYLMSGWLFLRNFNKQKINNISQRNYINSLVVLTIKIAKATDR
                     TVFAHQANIINTDLFCVIKKIDVLTLLII"
     gene            complement(350..2032)
                     /gene="glcA"
                     /locus_tag="ACHKTW_004424"
     CDS             complement(350..2032)
                     /gene="glcA"
                     /locus_tag="ACHKTW_004424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate permease GlcA"
                     /protein_id="HFO6465333.1"
                     /translation="MVTWTQMYMPMGGLGLSALVALIPIIFFFVALAVLRLKGHVAGA
                     ITLILSILIAIFAFKMPIDMAFAAAGYGFIYGLWPIAWIIVAAVFLYKLTVASGQFDI
                     IRSSVISITDDQRLQVLLIGFSFGALLEGAAGFGAPVAITGALLVGLGFKPLYAAGLC
                     LIANTAPVAFGALGVPILVAGQVTGIDPFHIGAMAGRQLPFLSVLVPFWLVAMMDGWK
                     GVKETWPAALVAGGSFAVTQFFTSNYIGPELPDITSALVSIVSLALFLKVWRPKNTET
                     AISMGQSAGAMVVNKPSSGGPVPSEYSLGQIIRAWSPFLILTVLVTIWTMKPFKALFA
                     PGGAFYSLVINFQIPHLHQQVLKAAPIVAQPTPMDAVFKFDPLSAGGTAIFIAAFISI
                     FILGVGIKKGIGVFAETLISLKWPILSIGMVLAFAFVTNYSGMSTTLALVLAGTGVMF
                     PFFSPFLGWLGVFLTGSDTSSNALFGSLQSTTAQQINVSDTLLVAANTSGGVTGKMIS
                     PQSIAVACAATGMVGRESELFRYTVKHSLIFASVIGVITLLQAYVFTGMLVS"
     gene            complement(2388..4559)
                     /gene="glcB"
                     /locus_tag="ACHKTW_004425"
     CDS             complement(2388..4559)
                     /gene="glcB"
                     /locus_tag="ACHKTW_004425"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HFO6465334.1"
                     /translation="MSQTITQGRLRIDANFKRFVDEEVLPGTGLDAAAFWRNFDEIVH
                     DLAPENRQLLAERDRIQAALDEWHRSNPGPVKDKAAYKSFLRELGYLVPQPERVTVET
                     TGIDSEITSQAGPQLVVPAMNARYALNAANARWGSLYDALYGSDIIPQEGAMVSGYDP
                     QRGEQVIAWVRRFLDESLPLENGSYQDVVAFKVVDKQLRIQLKNGKETTLRTPAQFVG
                     YRGDAAAPTCILLKNNGLHIELQIDANGRIGKDDPAHINDVIVEAAISTILDCEDSVA
                     AVDAEDKILLYRNLLGLMQGTLQEKMEKNGQQIVRKLNDDRHYTAADGSEISLHGRSL
                     LFIRNVGHLMTIPVIWDSEGNEIPEGILDGVMTGAIALYDLKVQKNSRTGSVYIVKPK
                     MHGPQEVAFANKLFTRIETMLGMAPNTLKMGIMDEERRTSLNLRSCIAQARNRVAFIN
                     TGFLDRTGDEMHSVMEAGPMLRKNQMKSTPWIKAYERNNVLSGLFCGLRGKAQIGKGM
                     WAMPDLMADMYSQKGDQLRAGANTAWVPSPTAATLHALHYHQTNVQSVQANIAQTEFN
                     AEFEPLLDDLLTIPVAENANWSVEEIQQELDNNVQGILGYVVRWVEQGIGCSKVPDIH
                     NVALMEDRATLRISSQHIANWLRHGILTKEQVQASLENMAKVVDQQNAGDPAYRPMAG
                     NFANSCAFKAASDLIFLGVKQPNGYTEPLLHAWRLREKESH"
     gene            complement(4581..4985)
                     /locus_tag="ACHKTW_004426"
     CDS             complement(4581..4985)
                     /locus_tag="ACHKTW_004426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlcG family protein"
                     /protein_id="HFO6465335.1"
                     /translation="MKTKVILSQQMASAIIAAGQEEAQKNNWSVSIAVADDGGHLLAL
                     SRMDDCAPIAAYISQEKARTAALGRRETKGYEEMVNNGRTAFVTAPLLTSLEGGVPVV
                     VDGQIIGAVGVSGLTGAQDAQVAKAAAAVLAK"
     gene            complement(4990..6213)
                     /gene="glcF"
                     /locus_tag="ACHKTW_004427"
     CDS             complement(4990..6213)
                     /gene="glcF"
                     /locus_tag="ACHKTW_004427"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HFO6465336.1"
                     /translation="MQTQLTEEMRQNARALEADSILRACVHCGFCTATCPTYQLLGDE
                     LDGPRGRIYLIKQVLEGNEVTLKTQEHLDRCLTCRNCETTCPSGVRYHNLLDIGRDIV
                     EQKVKRPLPERMLREGLRQVVPRPAVFRALTQVGLVLRPFLPEQVRAKLPAETVKAKP
                     RPPLRHKRRVLMLEGCAQPTLSPNTNAATARVLDRLGISVMSANEAGCCGAVDYHLNA
                     QEKGLARARNNIDAWWPAIEAGAEAILQTASGCGAFVKEYGQMLKNDALYADKARQVS
                     ELAVDLVELLREEPLEKLAVRGDKKLAFHCPCTLQHAQKLNGEVEKVLLRLGFTLTDV
                     PDSHLCCGSAGTYALTHPDLARQLRDNKMNALESGKPEMIVTANIGCQTHLASAGRTS
                     VRHWIEIVEQALEKE"
     gene            complement(6224..7276)
                     /gene="glcE"
                     /locus_tag="ACHKTW_004428"
     CDS             complement(6224..7276)
                     /gene="glcE"
                     /locus_tag="ACHKTW_004428"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HFO6465337.1"
                     /translation="MLRECDYSQALLEQVNQAISDKTPLVIQGSNSKAFLGRPVTGQT
                     LDVRCHRGIVNYDPTELVITARAGTPLVAIEAALESAGQMLPCEPPHYGEEATWGGMV
                     ACGLAGPRRPWSGSVRDFVLGTRIITGAGKHLRFGGEVMKNVAGYDLSRLMAGSYGCL
                     GVLTEISMKVLPRPRASLSLRREISLQEAINEIAQWQLQPLPISGLCYFDNALWIRLE
                     GGEGSVKAARELLGGEEVAGQFWQQLREQQLPFFSLPGTLWRISLPSDAPMMDLPGEQ
                     LIDWGGALRWLKSTAEDNQIHRIARNAGGHATRFSAGDGGFAPLSAPLFRYHQQLKQQ
                     LDPCGVFNPGRMYAEL"
     gene            complement(7276..8775)
                     /gene="glcD"
                     /locus_tag="ACHKTW_004429"
     CDS             complement(7276..8775)
                     /gene="glcD"
                     /locus_tag="ACHKTW_004429"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009339 - glycolate oxidase complex
                     [Evidence IEA];
                     GO_function: GO:0003973 - (S)-2-hydroxy-acid oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcD"
                     /protein_id="HFO6465338.1"
                     /translation="MSILYEERLDGALPDVDRTSVLMALREHVPGLEILHTDEEIIPY
                     ECDGLSAYRTRPLLVVLPKQMEQVTAILAVCHRLRVPVVTRGAGTGLSGGALPLEKGV
                     LLVMARFKEILDINPVGRRARVQPGVRNLAISQAVAPHNLYYAPDPSSQIACSIGGNV
                     AENAGGVHCLKYGLTVHNLLKIEVQTLDGEALTLGSDALDSPGFDLLALFTGSEGMLG
                     VTTEVTVKLLPKPPVARVLLASFDSVEKAGLAVGDIIANGIIPGGLEMMDNLSIRAAE
                     DFIHAGYPVDAEAILLCELDGVESDVQEDCERVNDILLKAGATDVRLAQDEAERVRFW
                     AGRKNAFPAVGRISPDYYCMDGTIPRRALPGVLEGIARLSQQYDLRVANVFHAGDGNM
                     HPLILFDANEPGEFARAEELGGKILELCVEVGGSISGEHGIGREKINQMCAQFNSDEI
                     TTFHAVKAAFDPDGLLNPGKNIPTLHRCAEFGAMHVHHGHLPFPELERF"
     gene            9026..9790
                     /gene="glcC"
                     /locus_tag="ACHKTW_004430"
     CDS             9026..9790
                     /gene="glcC"
                     /locus_tag="ACHKTW_004430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GlcC"
                     /protein_id="HFO6465339.1"
                     /translation="MKDERRPICEVVAESIERLIIDGVLKVGQPLPSERRLCEKLGFS
                     RSALREGLTVLRGRGIIETAQGRDSRVARLNRVQDTSPLIHLFSTQPRTLYDLLDVRA
                     LLEGESARLAATLGTQADFVVITRCYEKMLAASENNKEISLIEHAQLDHAFHLAICQA
                     SHNQVLVFTLQSLTDLMFNSVFASVNNLYHRPQQKKQIDRQHARIYNAVLQRLPHVAQ
                     RAARDHVRTVKKNLHDIELEGHHLIRSAVPLEMNLS"
     gene            complement(9797..>10921)
                     /gene="yghO"
                     /locus_tag="ACHKTW_004431"
     CDS             complement(9797..>10921)
                     /gene="yghO"
                     /locus_tag="ACHKTW_004431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YghO"
                     /protein_id="HFO6465340.1"
                     /translation="MIKIEKVINKNDLKAFIAFPSSLYPDDPNWIPPLFIERNEHLSA
                     KNPGTDHIIWQAWVAKKAGQIVGRITAQIDTLHRERYGEDTGHFGMIDAIDDPQVFAA
                     LFGAAEAWLKSQGASKISGPFSLNINQESGLLIEGFDTPPCAMMPHGKPWYAAHIEQL
                     GYHKGIDLLAWWMQRTDLTFSPALKKLMDQVRKKVTIRCINRQRFAEEMQILREIFNS
                     GWQHNWGFVPFTEHEFATMGDQLKYLVPDDMIYIAEIDSAPCAFIVGLPNINEAIADL
                     NGSLFPFGWAKLLWRLKVSGVRTARVPLMGVRDEYQFSRIGPVIALLLIEALRDPFAR
                     RKIDALEMSWILETNTGMNNMLERIGAEPYKRYRLYEKQI"
BASE COUNT         2576 a         3052 c         2741 g         2552 t
ORIGIN      
        1 ataaccccta tttgatgtct ggctggctat ttttacgaaa tttcaataaa caaaaaatta
       61 acaacatttc acaacgcaac tatataaatt cgttggtagt tttaacgatt aagattgcaa
      121 aagcaacaga tagaacggtg tttgctcatc aagcgaacat aattaataca gacttgtttt
      181 gcgtgataaa aaaaatagat gttctcacgc tcttaattat ttagcaggtt atctgaacac
      241 aaaacattat tgccgcatgg ataataaagc gagaaataat tttcaattaa taagacagaa
      301 taatgtaaat gaagccggat gatattaacg atcatccggc attattgatt tacgagacca
      361 acattccagt aaacacatac gcctgcaaca acgtgataac gccgataacg ctggcaaaaa
      421 tcagactgtg cttcacggtg tagcggaata gttcagattc tcggcccacc atgcccgtcg
      481 cggcgcaggc cacggcgata gattgcgggg agatcatctt gccagttacg ccgccgctgg
      541 tatttgctgc caccagcaga gtgtcagaga cgttgatttg ctgcgccgtg gtcgattgca
      601 gtgaaccaaa cagggcgttt gaagaagtgt ccgagccggt aaggaatacc cccagccagc
      661 cgaggaacgg tgagaaaaac gggaacatca cgcctgtacc tgccagtacc agcgccaacg
      721 tggtggacat gccggaatag ttggtgacga aggcgaacgc cagcaccatg ccaatcgaca
      781 atatcggcca cttcagacta attagcgttt cggcaaagac gccaatccct ttcttgatgc
      841 ccacgccgag gatgaagata gagataaacg ccgcaataaa aatagcggtg ccgccagccg
      901 agagagggtc gaatttaaac accgcatcca ttggcgttgg ttgggcgaca atgggtgccg
      961 ctttcaacac ttgttgatgc aaatgaggga tctggaaatt aatcaccagt gaataaaacg
     1021 cgccgcccgg agcaaataac gctttaaacg gcttcatggt ccagatggtg accagcaccg
     1081 ttaagattaa aaacggtgac cacgcacgaa tgatttgccc cagactatat tctgaaggca
     1141 cgggaccgcc agaagatggc ttatttacca ccatcgcacc tgcggattgc cccatgctga
     1201 ttgccgtttc ggtatttttc ggccgccaga ctttaaggaa taaagcgagt gagacgatac
     1261 tcaccagcgc cgaagtaata tccggcagtt ccggaccaat atagttagag gtaaagaact
     1321 gagtgacagc aaagcttccc ccagcaacca gcgccgctgg ccacgtctct ttcacccctt
     1381 tccagccgtc catcattgct accagccaga acggcacaag aaccgacagg aacggtaact
     1441 gacgtcccgc cattgcgcca atgtggaacg gatcgattcc cgttacctga ccggcgacca
     1501 gaatcggcac gcccaacgca ccaaacgcca ctggcgcagt gttggcaatc agacacagcc
     1561 ccgccgcgta taacggtttg aagcccaggc ccaccagcag cgcaccggta atcgccaccg
     1621 gcgcaccaaa gccagccgct ccttccagta acgcaccaaa ggagaaacca atcagtaaca
     1681 cctgcaaacg ctgatcgtcg gtgatggaga taaccgagct gcggataata tcgaactgac
     1741 cgctggcaac ggttaattta tagaggaaca ccgccgcgac aataatccac gcaataggcc
     1801 ataatccata aataaagcca tagcccgcag cagcaaaagc catatcaatc ggcattttaa
     1861 aggcgaatat tgcaatcagg atagataata taagggttat tgctccagcg acatgtcctt
     1921 tcagacgtaa taccgcgagt gcaacgaaga agaatattat cgggatcagg gcgaccagag
     1981 cggatagccc cagtcctccc atcggcatat acatttgggt ccaggtaacc atattgttca
     2041 gtctcttatt atctttatat gcttgatata cttaaggttg taataagcaa aagaggactg
     2101 aactgtaaaa tataggcgtt atactttaca gcaacagtac gccgctaacg caattgctac
     2161 ctctggcata acaagtatat cgggtaaggg tttctgttct gcacacgcag gcgcagagta
     2221 tcgttaagat gtccatattg ttgttatagg cccgctagta atgcgctacg ggtatttaat
     2281 attgttaaac cctgataatc gctccggtta tttccgggat aaatgtacta ccgcagttac
     2341 tatcatagcc ccgacaataa aacttgccgg ggcttttttt gacgctatta atgactttct
     2401 ttttcgcgta aacgccaggc gtgtaataac ggttcggtat agccgtttgg ctgtttcacg
     2461 ccgaggaaga ttaaatcgct ggcagcttta aaagcacacg agttagcgaa attccccgcc
     2521 atcggacgat aagccggatc gccagcgttt tgctgatcaa ccactttcgc catattctcc
     2581 agcgacgcct gcacctgttc tttggtcaga ataccgtgac gtaaccagtt ggcgatatgc
     2641 tggctggaga tacgcagcgt tgcgcggtct tccatcagcg ccacattgtg aatatccggc
     2701 acttttgaac aaccaatccc ctgctccacc cagcgcacca cgtaccccag aatcccctgc
     2761 acgttgttat ccagctcttg ttggatctct tccaccgacc agttagcgtt ttcagccacc
     2821 ggaatagtca gcagatcgtc cagcagcggt tcaaattcag cattgaactc ggtctgggca
     2881 atgttggctt gcacgctctg tacgttggtt tggtggtagt gcagcgcatg gagcgtagca
     2941 gcggttggtg acggaaccca ggctgtgttt gccccggcac gcagttggtc gcccttctgg
     3001 ctgtacatgt ctgccatcag gtccggcatt gcccacatgc ctttaccaat ttgcgcttta
     3061 ccgcgcagcc cacagaacag accggaaagc acgttattac gctcgtaggc tttgatccac
     3121 ggcgtcgatt tcatctggtt tttacgcagc atcgggccag cttccatcac cgaatgcatt
     3181 tcatcgccgg tacggtcgag gaaaccggta ttgatgaacg ccacgcggtt gcgcgcctga
     3241 gcgatacagc tacgcaagtt cagcgaggtc cgacgttctt catccataat gcccattttc
     3301 agggtattcg gtgccatacc gagcattgtc tcaatgcggg taaacagttt gttggcgaac
     3361 gccacttcct gcggaccgtg cattttcggt ttcacaatat agacgctgcc agtgcgcgag
     3421 tttttctgca cttttaaatc atagagggca atcgcgccag tcatgacgcc atcaagaatg
     3481 ccttccggga tttcattgcc ttcgctgtcc caaatcacag gaatggtcat caaatgaccc
     3541 acgttgcgga taaacagcag cgagcgtccg tgcagagaaa tttcagagcc atcggcggcg
     3601 gtgtaatgac gatcgtcatt cagtttacgc acgatttgct gaccgttttt ctccattttc
     3661 tcttgcagag tcccctgcat caggcccagc aggttgcggt acagcaggat tttatcttcc
     3721 gcatcaaccg ccgcgaccga atcttcgcag tcgagaatgg tactgatggc cgcttcgacg
     3781 ataacatcgt tgatgtgcgc cggatcgtct ttgccaatcc gcccgttggc atcaatttgc
     3841 agttcaatat gcaggccgtt atttttcagc aaaatgcagg tcggcgcagc ggcatcgcca
     3901 cggtaaccga caaactgcgc cggggtacgt aacgtggttt ctttaccatt tttcaactgg
     3961 atgcgtaatt gtttatcgac caccttaaac gccaccacat cctgatagct gccgttttcc
     4021 agcggtagag attcatcgag gaaacgccga acccaggcga taacctgctc accgcgttgc
     4081 ggatcgtagc cgctgaccat cgccccttcc tgcgggatga tgtcgctgcc gtataacgca
     4141 tcgtacagtg agccccagcg agcgttcgcc gcgttcagcg cgtagcgggc gttcattgcc
     4201 ggaaccacca gctgtggccc cgcctgactg gtgatttcgc tgtcaatgcc cgtggtttcc
     4261 accgtcacgc gctccggttg cggcaccagg tagcccagtt cacgcaggaa agatttatag
     4321 gccgctttat cttttaccgg ccccggattg ctgcgatgcc actcatcaag cgccgcctga
     4381 atgcgatcgc gttctgccag caactgacga ttttctggtg ccagatcgtg aacgatttca
     4441 tcaaaattgc gccagaacgc cgcagcgtcc agccctgttc ccggtaaaac ttcttcatcc
     4501 acaaaacgtt taaaattggc gtcaatgcgt aaacggccct gggttatggt ttgactcatt
     4561 gtttatctcc tcgttttcgc ttatttcgcc aacaccgctg ctgccgcttt cgcgacctgc
     4621 gcatcctgtg ctccggttaa accagaaacg cccacggcac caataatttg cccatcaaca
     4681 acaaccggta cgccaccttc cagcgacgtc agtaacggcg cagtcacgaa cgcggtacgt
     4741 ccgttgttca ccatctcttc atagccctta gtttcacgac gccccagcgc ggcggtacgc
     4801 gctttctcct gggagatgta agccgcaatc ggcgcgcaat cgtccatgcg acttaacgcc
     4861 agcagatgac cgccgtcatc ggcaacagca atggaaacag accagttatt tttctgcgcc
     4921 tcttcctgac ctgcggcaat aattgcactc gccatttgct ggctaagaat gactttagtt
     4981 ttcatttttt tattcctttt caagggcttg ttctacaatt tcaatccagt gacgcacaga
     5041 ggtacgaccg gcgctcgcca gatgcgtctg gcaaccaatg ttggcggtga cgatcatttc
     5101 cggtttgccg ctttccagcg cattcatttt gttatcccgc agctggcgtg ccagatcggg
     5161 atgcgttaac gcatatgttc ccgctgaacc gcagcacaga tggctgtcgg gaacgtccgt
     5221 taaggtaaat ccaagacgaa gcaacacttt ttccacttcg ccgttcagct tttgcgcatg
     5281 ttgtagggta cacgggcagt ggaaggccag ttttttatcg ccgcgaactg ccagtttttc
     5341 cagcggttcc tcgcgcagaa gctcgactaa atcgaccgcc agttcactga cttgacgcgc
     5401 tttatcggca tataacgcat cgtttttcag catctgcccg tactctttga caaacgcgcc
     5461 gcaaccgctg gcggtttgca aaattgcctc ggcacctgct tcaatcgcgg gccaccaggc
     5521 atcaatatta ttgcgcgccc gtgccagccc tttctcctgc gcattaagat gatagtccac
     5581 cgcgccacaa cagcctgctt cgttagctga catgacgctg atccccagac gatccagcac
     5641 tcgcgcagtt gccgcgttgg tgttgggcga aagcgtaggc tgggcgcagc cttccagcat
     5701 taaaacccga cgcttatggc gcagcggcgg acgcggttta gctttcaccg tttcagcagg
     5761 cagttttgct ctgacctgtt ccggtaaaaa cggtcgcagc accagcccta cctgcgtcag
     5821 cgcacggaag accgccggac gcggcactac ctggcgcaat ccttcgcgca gcattcgctc
     5881 cggcagtggg cgtttcactt tctgctcgac aatatcacgc ccgatatcca gcaaattgtg
     5941 atagcgcaca ccagaaggac aggtggtttc acaattgcgg caagtgaggc agcgatcgag
     6001 atgctcctgt gttttaagcg tgacttcgtt gccttccagc acctgtttaa tcagatagat
     6061 gcgcccgcgc ggcccgtcca gttcatcgcc cagaagctga taggttgggc aggttgcggt
     6121 acaaaatccg cagtgaacac aggcgcgcag gatgctgtcg gcttccagcg cgcgcgcgtt
     6181 ctgccgcatc tcttcagtta attgggtttg catagcctgc tcctcaaagt tccgcgtaca
     6241 tgcgaccggg gttaaacacg ccgcaagggt cgagctgctg tttaagctgc tggtgatagc
     6301 ggaataaagg agccgatagc ggggcaaagc caccatctcc ggcactaaag cgggtcgcat
     6361 gaccgccagc gttgcgggcg atgcgatgga tttgattgtc ctcggctgtc gatttcagcc
     6421 agcgtaacgc tccgccccag tcgatcagtt gctcgccggg taaatccatc atcggcgcat
     6481 cgctgggtaa tgaaatgcgc cataaggtac ctggtaacga gaagaacggc agttgttgtt
     6541 cacgcaattg ctgccagaac tgacccgcaa cctcttcgcc ccccagcagt tcacgcgccg
     6601 cttttaccga tccttcgccg ccctcaaggc ggatccacaa cgcattgtcg aagtaacata
     6661 agccactaat gggtagcggc tggagttgcc actgggcgat ttcgttaatg gcttcttgca
     6721 ggctgatttc ccgacgcagg ctcagggagg cgcgcggtcg cggtaacact ttcatcgaga
     6781 tttcagtgag cacgccaaga caaccgtagc ttccggccat taaccgtgag agatcgtatc
     6841 cggcaacgtt tttcatcact tcgccaccaa aacgcagatg ttttccagcg ccggtaatga
     6901 tgcgcgtgcc gaggacaaaa tcgcggaccg aaccgctcca cgggcgacgc ggccccgcca
     6961 gcccgcaggc gaccatcccg ccccaggtgg cttcttcacc ataatgcggc ggctcgcagg
     7021 ggagcatttg cccggcgctc tccagcgccg cttcaatcgc caccagcggc gttccggcac
     7081 gcgcggttat caccagctcg gtcgggtcgt aattaacaat gccgcgatga caacgcacat
     7141 ccagcgtttg cccggtgaca gggcgaccta aaaaggcttt gctattgctg ccctgaatca
     7201 ccagcggcgt tttatcgcta attgcctgat tcacctgctc cagcagcgcc tggctgtaat
     7261 cacactcgcg tagcatcaga aacgctccag ttcagggaaa ggtaaatgac cgtgatgcac
     7321 atgcatggca ccaaattcag cacagcggtg tagcgtggga atgtttttcc cagggttcag
     7381 caaaccatcg gggtcaaacg ccgccttgac cgcatggaag gtcgtgattt catcgctgtt
     7441 gaactgggcg cacatttgat tgattttttc tcgcccgatg ccatgttccc cactgatgct
     7501 gccgccaact tcaacgcaga gttcgaggat cttcccaccc agctcttccg cgcgggcaaa
     7561 ttcaccgggt tcgttggcat cgaaaaggat taacgggtgc atgttgccat ctccggcatg
     7621 aaagacgttg gcaacacgta aatcatattg ctgcgataaa cgggcaatgc cttccagtac
     7681 gccaggcagg gcgcgacgcg ggatggtgcc atccatgcag tagtaatccg gggagatacg
     7741 tcctaccgcc gggaacgcat ttttgcgacc ggcccagaaa cgtacgcgct ctgcttcgtc
     7801 ctgtgccaga cggacgtcag tcgcgcccgc tttcaacaag atgtcgttaa cccgctcgca
     7861 gtcttcctgt acgtcagact ccacgccgtc cagctcgcat aacaaaatcg cttcggcgtc
     7921 gacgggataa ccggcatgaa taaaatcttc cgccgcgcgg atcgacaggt tatccatcat
     7981 ctccagcccg ccggggataa tgccattggc gatgatgtca ccaactgcaa gtccggcttt
     8041 ttctaccgag tcaaagctgg ctaacagaac ccgcgccacg ggcggcttcg gcagcagttt
     8101 taccgtcact tcggtggtca cgccgagcat accttccgat ccggtgaaca gcgccagcag
     8161 gtcaaaacca ggtgaatcca gcgcgtccga tcccagcgtc agcgcctcgc cgtccagcgt
     8221 ttgcacttca attttcagca ggttatgtac ggtcagacca tatttcaggc agtggacgcc
     8281 gccggcattt tctgccacgt tgccgccaat ggaacaggcg atttgtgagg aagggtccgg
     8341 tgcgtagtag agattatgcg gtgcaacggc ctgggagatc gccaggttac gcacgcctgg
     8401 ctgcacgcgc gcgcggcgac caacggggtt aatgtcgagg atctctttaa agcgcgccat
     8461 caccaacaac acaccttttt ccagcggcag cgcgccacca gaaagcccgg tgcctgcacc
     8521 acgggtcacc accggtacac gcaggcgatg gcagacagca agaatcgctg tcacctgttc
     8581 catttgctta ggcagaacaa ccagtaatgg acgcgtgcga tacgcgctca acccgtcaca
     8641 ctcgtaagga atgatctcct catcggtatg caggatctca agtccaggga catgctcacg
     8701 cagtgccatc agtaccgatg tgcggtcgac atcgggtaaa gcgccatcaa gacgctcttc
     8761 gtacaagatg ctcatgagta ggcttcgctt tgttgtgttg tgtggcagct gatttttgcg
     8821 cgctgcttct gtgaacagtt attaagcggg cttttcgttt tcgtctatct ctttagctac
     8881 cggtcagacc attttttttc cagctctgtg accttgcctt ggttaactca atgttaaatt
     8941 gatgtaacat aatcacttac gtgatgtgcg tgttttgcga gttaagaaca gaaaaattgg
     9001 tcctacctgt gcacgaggtc cgggaatgaa agatgaacgt cgccctattt gcgaagtggt
     9061 tgcagagagt atcgaacggt taattatcga cggcgtactg aaggtcggtc agccacttcc
     9121 ctcggaacgt cgactgtgtg aaaagctcgg cttctcacgc tccgcactgc gtgaagggct
     9181 gaccgtgctg cgcgggcgcg ggattattga aacggcgcag ggtcgcgatt ctcgtgtcgc
     9241 acggcttaat cgggtgcagg acaccagccc gctgatccat ctgttcagta cgcagccgcg
     9301 aacgctgtac gatctgctcg acgttcgcgc attactggag ggcgaatcgg caaggctggc
     9361 ggcaacgctg ggaacgcagg ctgattttgt tgtgataacc cgctgttatg aaaaaatgct
     9421 cgccgccagt gagaacaaca aagagatttc gctgatcgaa catgcgcagt tggatcacgc
     9481 tttccatctc gccatttgtc aggcttctca caatcaggtg ctggtgttta cgctgcaatc
     9541 attgaccgat ctgatgttta attcagtgtt tgccagcgta aataatctct accatcgacc
     9601 acagcaaaaa aagcagatcg atcgccagca tgcgaggatc tacaacgcgg tgttgcagcg
     9661 gctgccgcac gtcgcccagc gcgcagcacg cgatcatgtg cggaccgtga aaaagaatct
     9721 ccacgatatc gagctggaag gccaccattt gattcgctcg gcggtgccgc tggagatgaa
     9781 cctgagttag ctggtattaa atctgctttt catacaatcg gtaacgcttg tacggctccg
     9841 ccccaatgcg ttccagcatg ttattcatgc ctgtattggt ttcgaggatc catgacatct
     9901 ccagcgcatc gatcttccgg cgggcaaacg gatcgcgtaa ggcttcaatc aataacagcg
     9961 caatcaccgg gccgatgcgg ctgaactgat actcgtcgcg cacgcccatc agcggtactc
    10021 gcgcagttcg cacaccgctg actttcaaac gccacagcaa ttttgcccag ccgaagggaa
    10081 agagcgatcc gttcagatcg gcaatcgcct cgttgatgtt cggcaagccg acaataaacg
    10141 cgcagggtgc agaatcaatc tcagcgatat agatcatatc gtccggcacc agatatttaa
    10201 gttgatcgcc catggtcgcg aattcatgtt cggtaaacgg cacaaatccc cagttgtgct
    10261 gccagccaga gttgaaaatc tcacgcagga tctgcatttc ttcggcaaac cgctgacgat
    10321 tgatgcagcg aatggtcacc tttttgcgca cctgatccat cagttttttt agcgccggag
    10381 agaaagtgag atcggttcgc tgcatccacc acgccagtaa atcaatgcct ttgtgataac
    10441 ccagttgttc aatatgcgcg gcataccacg gtttgccgtg tggcatcatc gcacagggtg
    10501 gtgtgtcaaa accttcaatc agtaatccgc tttcctgatt gatattcagg ctgaaaggac
    10561 cgctgatctt acttgcacct tgtgacttca accacgcttc cgctgcgcca aacaacgcgg
    10621 caaaaacctg cggatcatca atggcgtcaa tcatgccgaa atgaccggta tcttcaccgt
    10681 aacgctcacg gtgcaaggta tctatttgcg cggtaatacg cccaactatc tgccccgctt
    10741 tttttgccac ccacgcctgc cagatgatat ggtccgtccc cggatttttc gcagacaaat
    10801 gctcgttgcg ttctatgaat aaagggggta tccagtttgg atcgtcggga taaagtgatg
    10861 acggaaaagc gataaatgcc ttaaggtcat ttttattaat tactttttca attttaatca
    10921 t
//
LOCUS       DAZFQD010000162        10713 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.162, whole genome shotgun sequence.
ACCESSION   DAZFQD010000162 DAZFQD010000000
VERSION     DAZFQD010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10713
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.162"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            52..1434
                     /gene="dosC"
                     /locus_tag="ACHKTW_004432"
     CDS             52..1434
                     /gene="dosC"
                     /locus_tag="ACHKTW_004432"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
                     /protein_id="HFO6465341.1"
                     /translation="MEMYFKRMKDEWTGLVEQADPLIRAKAAEIAVAHAHYLSIEFYR
                     IVRIDPHAEEFLSNEQVERQLKSAMERWIINVLSAQVDDVERLIQIQHTVAEVHARIG
                     IPVEIVEMGFRVLKKILYPVIFSSDYSAAEKLQVYHFSINSIDIAMEVMTRAFTFSDS
                     SASKEDENYRIFSLLENAEEEKERQIASILSWEIDIIYKVLLDSDLGSSLPLSQADFG
                     LWFNHKGRHYFSGIAEVGHISRLIQDFDGIFNQTMRNTRNLNNRSLRVKFLLQIRNTV
                     SQIITLLRELFEEVSRHEVGMDVLTKLLNRRFLPTIFKREIAHANRTGTPLSVLIIDV
                     DKFKEINDTWGHNTGDEILRKVSQAFYDNVRSSDYVFRYGGDEFIIVLTEASENETLR
                     TAERIRSRVEKTKLKAANGEDIALSLSIGAAMFNGHPDYERLIQIADEALYIAKRRGR
                     NRVELWKASL"
     gene            1459..3858
                     /gene="dosP"
                     /locus_tag="ACHKTW_004433"
                     /gene_synonym="pdeO"
     CDS             1459..3858
                     /gene="dosP"
                     /locus_tag="ACHKTW_004433"
                     /gene_synonym="pdeO"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-sensing cyclic-di-GMP phosphodiesterase
                     DosP"
                     /protein_id="HFO6465342.1"
                     /translation="MKLTDADNAADGIFFPALEQNMMGAVLINENNEVMFFNPAAEKL
                     WGYKREEVIGNNIDMLIPRDLRPAHPEYIRHNREGGKARVEGMSRELQLEKKDGSKIW
                     TRFALSKVSAEGKVYYLALVRDASVEMAQKEQTRQLIIAVDHLDRPVIVLDPERHIVQ
                     CNRAFTEMFGYCISEASGMQPDTLLNIPEFPADNRIRLQQLLWKTARDQDEFLLLTRT
                     GEKIWIKASISPVYDVLAHLQNLVMTFSDITEERQIRQLEGNILAAMCSSPPFHEMGE
                     IICRNIESVLNESHVSLFALRNGMPIHWASSSHGAEVQNAQSWSATIRQRDGAPAGIL
                     QIKTSSGAETSAFIERVADISQHMAALALEQEKSRQHIEQLIQFDPMTGLPNRNNLHN
                     YLDDLVDKAVSPVVYLIGVDHIQDVIDSLGYAWADQALLEVVNRFREKLKPDQYLCRI
                     EGTQFVLVSLENDVSNITQIADELRNVVSKPIMIDDKPFPLTLSIGISYDVGKNRDYL
                     LSTAHNAMDYIRKNGGNGWQFFSPAMNEMVKERLVLGAALKEAISNNQLKLVYQPQIF
                     AETGELYGIEALARWHDPQHGHVPPSRFIPLAEEIGEIENIGRWVIAEACRQLAEWRS
                     QNIHIPALSVNLSALHFRSNQLPNQVSDAMHAWGIDGHQLTVEITESMMMEHDTEIFK
                     RIQILRDMGVGLSVDDFGTGFSGLSRLVSLPVTEIKIDKSFVDRCLTEKRILALLEAI
                     TSIGQSLNLTVVAEGVETKEQFEMLRKIHCRVIQGYFFSRPLPAEEIPGWMSSVLPLK
                     I"
     gene            4116..4697
                     /gene="ddpX"
                     /locus_tag="ACHKTW_004434"
     CDS             4116..4697
                     /gene="ddpX"
                     /locus_tag="ACHKTW_004434"
                     /EC_number="3.4.13.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
                     /protein_id="HFO6465343.1"
                     /translation="MSDTTELVDLAVIFPDLEIELKYACADNITGKAIYQQARCLLHK
                     DAITALAKSISIAQLSGLQLVIYDAYRPQQAQAMLWQACPDPQYVVDVTVGSNHSRGT
                     AIDLTLRDEHGNILDMGAGFDEMHERSHAYHPSVPPAAQRNRLLLNAIMTGGGFVGIY
                     SEWWHFELPQAASYPLLADQFTCFISPGTQHVS"
     gene            4711..6261
                     /locus_tag="ACHKTW_004435"
     CDS             4711..6261
                     /locus_tag="ACHKTW_004435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFO6465344.1"
                     /translation="MKRSIPFRPTLLALVLATNFPVAHAAVPKDMLVIGKAADPQTLD
                     PAVTIDNNDWTVTYPSYQRLVQYKTDGDKGSTDVEGDLASSWKASDDQKEWTFTLKDN
                     AKFADGTPVTAEAVKLSFERLLKIGQGPAEAFPKDLKIDAPDEHTVKFTLSQPFAPFL
                     YTLANDGASIINPAVLKEHAADDARGFLAQNTAGSGPFMLKSWQKGQQLVLVPNPHYP
                     GNKPNFKRVSVKIIGESASRRLQLSRGDIDIADALPVDQLNALKQENKVNVAEYPSLR
                     VTYLYLNNSKAPLNQADLRRAISWSTDYQGMVNGILSGNGKQMRGPIPEGMWGYDATA
                     MQYNHDETKAKAEWDKVTSKPTSLTFLYSDNDPNWEPIALATQSSLNKLGINVKLEKL
                     ANATMRDRVGKGDYDIAIGNWSPDFADPYMFMNYWFESDKKGLPGNRSFYENSEVDKL
                     LRNALATTDQTQRTRDYQQAQKIVIDDAAYVYLFQKNYQLAMNKEVKGFVFNPMLEQV
                     FNINTMSK"
     gene            6263..7285
                     /locus_tag="ACHKTW_004436"
     CDS             6263..7285
                     /locus_tag="ACHKTW_004436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO6465345.1"
                     /translation="MTFWSILRQRCWGLVLVVAGVCVITFIISHLIPGDPARLLAGDR
                     ASDAIVENIRQQLGLDQPLYVQFYRYVSDLFHGDLGTSIRTGRPVLEELRIFFPATLE
                     LAFGALLLALLIGIPLGILSAVWRNRWLDHLVRIMAITGISTPAFWLGLGVIVLFYGH
                     LQILPGGGRLDDWLDPPTHVTGFYLLDALLEGNGEVFFNALQHLILPALTLAFVHLGI
                     VARQIRSAMLEQLSEDYIRTARASGLPGWYIVLCYALPNALIPSITVLGLALGDLLYG
                     AVLTETVFAWPGMGAWVVTSIQALDFPAVMGFAVVVSFAYVLVNLVVDLLYLWIDPRI
                     GRGGGE"
     gene            7282..8178
                     /gene="ddpC"
                     /locus_tag="ACHKTW_004437"
     CDS             7282..8178
                     /gene="ddpC"
                     /locus_tag="ACHKTW_004437"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HFO6465346.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLVWMLKGSPLTVTGAVIIVLML
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLINWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTAV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            8175..9161
                     /locus_tag="ACHKTW_004438"
     CDS             8175..9161
                     /locus_tag="ACHKTW_004438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO6465347.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGDVHALNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLQTGSYCVHRGQISLLGDDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTLRIGLQMMDVIRHHQPISRREARAKAIALLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIALAFSCEPQLIIADEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGVTADVIHHPRHPYTIGLLQCAPEHGVPRQLLPAIPGT
                     VPNLTHLPEGCAFRDRCYAAGAQCENVPALTACGDNNQRCACWYPQQEVISV"
     gene            9154..10080
                     /locus_tag="ACHKTW_004439"
     CDS             9154..10080
                     /locus_tag="ACHKTW_004439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HFO6465348.1"
                     /translation="MSDTLLTLRDVHINFPARKNWLGKTTEHVHAINGIDLQIRRGET
                     LGIVGESGCGKSTLAQLLMGMLQPSHGQYIRSGSQRIMQMVFQDPLSSLNPRLPVWRI
                     ITEPLWIAKRSSEQQRRALAEELAVQVGIRPEYLDRLPHAFSGGQRQRIAIARALSSQ
                     PDVIVLDEPTSALDISVQAQILNLLVTLQENHGLTYVLISHNVSVIRHMSDRVAVMYL
                     GQIVELGEAQQVLTAPAHPYTRLLLDSLPAIDKPLEEEWALRKTDLPGNRTLPQGCFF
                     YERCPLATHGCEVRQSLTIREDGREIRCWRAL"
     gene            complement(10136..10567)
                     /gene="osmC"
                     /locus_tag="ACHKTW_004440"
     CDS             complement(10136..10567)
                     /gene="osmC"
                     /locus_tag="ACHKTW_004440"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HFO6465349.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDAGFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
BASE COUNT         2616 a         2672 c         2770 g         2655 t
ORIGIN      
        1 gcgtttcgtt ttgattccgt ctgatttccc agccttataa gggtgtgaat tatggagatg
       61 tattttaaaa gaatgaaaga tgagtggacc ggacttgtcg aacaggcaga tcctctcatt
      121 cgtgctaaag ccgcagaaat tgccgttgcg catgctcatt atctgagtat cgagttttat
      181 cgaattgtcc gcatcgaccc gcatgccgaa gaattcttga gtaatgaaca agttgagcgg
      241 cagttgaaaa gtgcgatgga acgctggatt attaacgtgc tttctgccca ggttgacgat
      301 gtcgaaaggc taatacaaat ccagcatacc gtcgcggaag tgcatgcccg cataggaatt
      361 ccggtagaaa ttgtcgagat ggggtttcgg gtgctgaaaa aaatcctcta tccggtaatc
      421 ttctcttcgg attattccgc cgcagaaaaa cttcaggtct accatttctc gattaacagt
      481 attgatatcg ctatggaagt gatgacccgc gcgtttacct ttagtgacag tagtgcctca
      541 aaggaagatg aaaactatcg tatcttctcg ttactggaaa acgccgaaga agaaaaagaa
      601 cggcaaatag cctcaatact ttcatgggaa atagatatta tctataaagt cctgctggat
      661 tctgatttag gcagcagttt gcctttaagc caggctgatt ttggcctgtg gtttaaccat
      721 aaaggtcgac attattttag cggcattgcc gaagtaggcc atatctcccg tctgattcag
      781 gatttcgacg gtattttcaa tcaaaccatg cgtaacacca ggaatttgaa taacagaagt
      841 ttgcgggtga agtttctatt acagataaga aataccgtat cgcaaattat taccttgctg
      901 cgtgaattgt ttgaagaagt atcgcgccac gaagtcggta tggatgtact gacgaaatta
      961 cttaaccgcc gtttcctgcc gactatcttc aaacgcgaaa ttgcccatgc caaccggacc
     1021 ggtacaccgc tgtcagtgct gattattgac gttgataaat tcaaagagat caacgatacg
     1081 tggggccata acactggcga tgaaattctg cgtaaagtct ctcaggcctt ttatgacaac
     1141 gtccgcagta gtgattatgt tttccgctac gggggcgatg aatttatcat tgttttgact
     1201 gaagcttctg aaaacgaaac gttacgtacc gcagaacgta ttcgcagtcg ggtggagaaa
     1261 accaaactga aagccgcaaa cggcgaggat attgccctct cactttccat cggtgccgcc
     1321 atgtttaatg gtcatcctga ctatgagcgc ctcattcaaa tagccgatga agctctgtat
     1381 atcgctaaaa gacgaggtag aaaccgtgtt gaactctgga aagccagtct ttagatgcgc
     1441 caggatgcag aggtaatcat gaagctaacc gatgcggata atgccgccga tggcattttt
     1501 ttccccgccc ttgagcaaaa tatgatgggt gcggtgttaa ttaacgaaaa taatgaagtg
     1561 atgtttttca accccgccgc agagaagctc tgggggtaca aacgtgaaga agtcattggc
     1621 aataacattg atatgctgat tccgcgggat ttgcgtcctg cgcatcctga atacattcgt
     1681 cataaccgtg aaggcggtaa agcgcgtgtc gaggggatga gtcgggagct gcagctggag
     1741 aaaaaagacg gcagtaaaat ctggacccgt tttgcgctat cgaaagtgag cgccgagggg
     1801 aaagtttatt acctggcgct ggtacgggat gccagcgtag aaatggcgca aaaagaacag
     1861 actcgacaat tgattattgc cgttgaccat ctcgaccgac cggtgattgt cctcgatccg
     1921 gaacgccata ttgtgcagtg caatcgcgca tttaccgaaa tgtttggtta ctgcattagc
     1981 gaagccagcg gtatgcagcc cgatacactc ctgaacattc ctgaattccc tgccgataac
     2041 cgcattcgtt tacaacagtt gctatggaaa accgcccgcg atcaggatga atttctgctg
     2101 ttgacgcgca ccggtgaaaa aatctggatt aaagcctcta tcagcccggt ttatgacgtg
     2161 ctcgcgcatc tgcagaacct ggtaatgacc ttctcggata tcaccgaaga acggcaaatc
     2221 cgccagcttg aaggcaatat tctcgccgcc atgtgcagca gcccgccatt tcatgaaatg
     2281 ggggaaatca tttgtcgtaa catcgaatct gttctcaacg aatcgcatgt ttcgctgttc
     2341 gcactgcgca acgggatgcc gatacactgg gcgtcatctt cgcacggtgc agaagttcaa
     2401 aatgcgcaaa gctggtcagc gaccattcgt cagcgtgatg gcgcgcctgc ggggatcctg
     2461 caaattaaaa cctcgtcagg agcagaaacc agcgccttta tcgaacgcgt ggcagatatc
     2521 agtcagcata tggccgcgct ggcgctggaa caggaaaaaa gccgccagca tattgaacaa
     2581 ctcatccaat ttgatccgat gaccggtctg ccaaatcgca ataacctgca caattacctc
     2641 gatgacctgg tcgacaaagc cgtctctccg gtggtgtatc tcatcggtgt tgaccatatt
     2701 caggatgtga ttgatagcct tggctatgcg tgggccgatc aggcattgct ggaagtggtc
     2761 aatcgctttc gtgaaaaact caaaccggat cagtatctct gtcgtatcga aggtacgcag
     2821 tttgtcctcg tgagcctgga aaacgacgtc agtaacatta cccaaatcgc cgatgagcta
     2881 cggaatgtgg tcagcaagcc gataatgatt gacgataaac ccttcccgct taccttgagt
     2941 attggcatca gctacgacgt gggtaaaaac cgcgattacc tgctctccac tgctcacaat
     3001 gcaatggatt atattcgcaa gaatggcggt aacggctggc agttcttcag cccggcgatg
     3061 aacgaaatgg taaaagagcg tttggtttta ggcgcagcgc tgaaagaagc gattagcaat
     3121 aaccaactga aacttgttta ccagccgcaa atcttcgcag aaacgggtga actgtacggc
     3181 atcgaagccc ttgcgcgctg gcacgatccc cagcatggtc atgtgccccc ttcacggttt
     3241 attcctctcg cagaagagat tggtgaaatc gaaaatattg ggcgctgggt catcgcggaa
     3301 gcttgccgtc agttagcaga atggcgtagc cagaatattc atatcccggc gctttccgtt
     3361 aacttgtcgg cactgcactt tcgcagtaat caactgccta atcaggtgtc tgatgcaatg
     3421 cacgcctggg gtattgacgg ccaccagctg acggtagaaa tcacggaaag catgatgatg
     3481 gaacacgata ccgaaatctt taagcgcatt cagatcctgc gtgatatggg cgtgggctta
     3541 tcggtagatg attttggtac gggcttttcc ggattatccc gcttagtcag tcttccggta
     3601 acggaaatca aaattgacaa aagttttgtc gatcgttgtc tgaccgaaaa acgcatcctt
     3661 gccttacttg aagccattac cagcattggg caaagcctca atttaaccgt cgtggcggaa
     3721 ggcgtcgaaa ccaaagagca atttgagatg ctacgcaaga tccactgtcg cgttattcag
     3781 ggatatttct tttcccgccc cctacccgcc gaagaaattc caggctggat gagcagcgtg
     3841 ttaccgctga aaatctgaca aattcctctc gcccgcactc gcgggttttc atttaacgtg
     3901 acactgtcac ttaaatactg tgatttcagc tacgattccg ggagattcct tccttaactt
     3961 cccctcttgc accaaaactg cactacgctg cacaaatgtt gatcaaaagt taaaaaatat
     4021 aactttaagc cagcattctg gcgcttatcc cggcatggca tgagatctgc ataagcggaa
     4081 agcgcagcaa tttttgtctt atacaggaac cgtttatgtc ggataccacc gaactggttg
     4141 atttagccgt aatcttccct gatctggaga tcgaattgaa atacgcctgc gctgataaca
     4201 tcacaggtaa agctatttat cagcaagcgc gttgtctgct acacaaggat gcgattaccg
     4261 cgctggcgaa aagtatcagt atcgcccagc tgtcaggctt acaactggtg atttacgatg
     4321 cgtatcgccc acaacaagca caggcgatgt tgtggcaagc ctgcccagac ccgcaatatg
     4381 ttgttgatgt gacggtcggt tctaatcaca gccgtggcac ggcgatcgac ctgacgcttc
     4441 gtgatgagca cgggaacatc ctcgatatgg gcgcagggtt cgatgaaatg cacgagcgct
     4501 cccatgccta tcacccttcc gtcccgcccg ccgctcagcg caatcggctg ttgctgaatg
     4561 cgataatgac tggtggcggt tttgtcggta tctacagcga atggtggcac ttcgaattac
     4621 cccaggcagc gagttaccct ctgctcgctg atcaattcac ctgttttata tcccctggca
     4681 cacaacacgt cagttaatca ggaaactgtc atgaagagat cgataccgtt tcgtcccaca
     4741 ttgctcgcgc tcgtccttgc cacaaatttc ccggttgcgc acgccgccgt accgaaagat
     4801 atgctggtga ttggtaaggc cgccgatcca caaaccctcg acccggcggt aacaatagat
     4861 aataacgact ggacggtgac ctacccgtct tatcagcggc tggttcagta caaaacggac
     4921 ggtgataaag gctcaaccga cgttgaaggc gatctggcaa gtagctggaa agcgtctgac
     4981 gatcaaaaag agtggacgtt caccctgaag gacaatgcta aatttgccga tggcacaccg
     5041 gtcactgccg aagcagtaaa actctctttt gagcggctac taaaaatcgg ccaggggcca
     5101 gcagaagcat ttcccaaaga tttaaagatt gatgctcccg acgaacatac ggtgaagttt
     5161 acccttagcc agccattcgc accgttcctc tacacgctgg cgaatgacgg tgcatccatt
     5221 atcaatccgg cggtcttaaa ggaacatgca gcggatgatg ctcgcggctt cctcgcgcaa
     5281 aataccgccg gttccggacc atttatgctg aaaagctggc aaaaaggtca gcaattagtt
     5341 ctggtgccaa atccgcatta ccccggcaat aaaccgaact ttaagcgagt atcggtaaaa
     5401 attatcggtg aaagtgcctc ccgtcgcctg cagctctccc gtggcgacat tgacattgcc
     5461 gatgcgctgc cggtggatca actcaacgcc ctgaagcagg aaaataaagt caatgtggca
     5521 gagtatccgt cactgcgcgt tacctatctg tatctgaata acagcaaagc gcctcttaat
     5581 caggcggatc tgcgtcgggc catttcctgg tctaccgatt atcagggcat ggttaacggc
     5641 attctgagtg gtaacggaaa acagatgcgc ggcccgattc cggaaggcat gtggggctac
     5701 gatgcgacgg caatgcaata caaccatgac gaaacgaaag ccaaagccga atgggataaa
     5761 gtaacgagca aacccaccag cctgacgttt ctctactccg ataacgatcc gaactgggag
     5821 cctattgctc tggcgacaca atccagtctc aacaagctgg gcatcaatgt gaagctggaa
     5881 aagctggcga acgccaccat gcgcgacaga gtgggtaaag gtgattacga cattgcgatt
     5941 ggcaactgga gtccggattt tgccgacccg tatatgttta tgaattactg gtttgagtca
     6001 gacaaaaaag gtctgccggg taaccgctcg ttctatgaaa acagtgaggt cgataagtta
     6061 ctgcgcaatg cgcttgcgac caccgaccag acgcagcgta cccgggacta ccagcaggca
     6121 cagaaaatcg tcattgatga cgctgcttat gtgtacctgt tccagaaaaa ctaccaactg
     6181 gcgatgaaca aagaggtgaa aggctttgtg ttcaatccca tgctggaaca ggtcttcaat
     6241 atcaatacca tgagtaaata acgtgacctt ctggagtatt ttacgccaac gctgctgggg
     6301 gctggtgctc gtggtggcgg gcgtctgcgt gattacgttt attatctcgc atctgatccc
     6361 tggcgatccg gcgcggttac tggcgggtga ccgcgccagc gatgctatcg tggaaaatat
     6421 tcgccagcaa ctgggactgg accagccact gtatgtacag ttttaccgct acgtcagcga
     6481 cctgtttcat ggtgacctgg gaacatccat tcgtaccggg cgtccggtgc tggaagagtt
     6541 gcgtatattt ttcccggcga cgctggagct ggcttttggt gccctgctgc tggcactcct
     6601 gattggcatc ccgctgggca tactctctgc agtctggcga aatcgctggc tggatcatct
     6661 ggtgcgaata atggccatta ccggaatctc cacacctgcg ttctggcttg ggctgggcgt
     6721 cattgtgctg ttttatggtc atctgcaaat tcttcccggc ggcggaaggc ttgatgactg
     6781 gctggatcca ccaacgcacg ttaccggctt ttatctgctc gatgcactgc ttgaaggcaa
     6841 cggtgaagtc ttcttcaatg cgttgcaaca tctcatctta ccggcattaa cgctggcgtt
     6901 cgttcacctg ggaattgtcg ctcgccagat ccgctcagcg atgctggaac aattgagtga
     6961 agactacatt cgtaccgccc gggccagcgg cttgcccggc tggtatatcg ttttatgtta
     7021 tgcgctaccc aatgcgttga tcccatcgat taccgtattg ggtttggcgc tgggcgattt
     7081 gttgtatggc gcagtgctca ccgaaaccgt ttttgcctgg cccggaatgg gtgcatgggt
     7141 agtaacatca atacaggcgc tcgacttccc ggcagtgatg ggctttgccg tcgtggtttc
     7201 atttgcttat gtgctggtca acctggtggt ggatttgctc tatttgtgga ttgatccgcg
     7261 tatcggacgt ggaggtggtg aatgatgcta agcgaggaaa cgtccgccgt acgcccacaa
     7321 aaacaaacgc gatttaacgg tgcaaaactg gtatggatgc tgaaaggcag tccgctcacc
     7381 gtgaccggcg cagtcatcat tgtattaatg ctattgatga tgattttttc accgtggctg
     7441 gcgacgcatg atcccaacgc cattgattta accgcccgcc ttttgccgcc ttctgcggcg
     7501 cactggtttg gcaccgatga agtgggacgc gatctgttca gccgcgtact ggtcggcagt
     7561 cagcaatcaa ttctcgccgg attagtcgtg gttgccattg cgggtatgat tggttcgcta
     7621 ctcggatgtc tatccggtgt gcttggcgga cgcgcagacg ccatcatcat gcgcatcatg
     7681 gacattatgc tgtcgattcc ttcgttggta ctgacaatgg cactggctgc cgctctcggg
     7741 ccgagtttat ttaacgccat gctggcgatt gctattgtgc gaattccctt ttatgtgcgc
     7801 ctggcgcggg ggcaagcatt agttgtacgc cagtatactt atgttcaggc ggcgaaaacc
     7861 tttggcgcgt ctcgttggca tctgatcaac tggcatattt tacgtaactc cctgccgccg
     7921 ctgatcgtgc aggcatcgct ggatatcggt agcgcgattt taatggccgc cacgttggga
     7981 tttattggcc tgggtgcaca acaaccgagt gctgaatggg gcgcgatggt agcgaatggt
     8041 cgcaactatg tactcgatca gtggtggtat tgcgcatttc cgggggcagc gattttgctt
     8101 accgccgtcg ggtttaatct ctttggtgat ggtattcgcg atctgcttga cccgaaagca
     8161 ggaggaaagc agtcatgacc caacccgttc tggacattca acagctgcat ttgagtttcc
     8221 ccggttttaa cggtgatgtt cacgcgctca acaatgtgtc cttgcagatt aaccgcggtg
     8281 aaattgtcgg tctggtggga gaatccggct caggtaaatc agtcaccgca atgctgatta
     8341 tgcgtctgct acagacgggc agttattgcg tacatcgggg gcagatttca ctgctgggag
     8401 acgatgttct taacgctcgg gaaaaacagc ttcgtcagtg gcgcggcgca cgagtggcga
     8461 tgatctttca ggaaccgatg accgccctca atccgacact tcgaataggt cttcagatga
     8521 tggacgtgat ccgccatcat caaccaataa gtcgtcggga agccagagct aaagcgattg
     8581 ccctgctgga agagatgcaa atcccggatg ccgtggaagt tatgtcgcgc tatccgtttg
     8641 agctttcagg tggtatgcgc cagcgggtaa tgattgcgct ggcattctcc tgcgagccgc
     8701 aattgattat tgccgacgaa ccgactacgg cgctggacgt cacggtgcag ttgcaggtac
     8761 tgcgtttgct taaacataaa gcccgcgcca gtggaactgc ggttctgttc atcagccatg
     8821 atatggccgt ggtgtcgcaa ctgtgcgata gcgtttacgt gatgtatgcc ggaagcgtaa
     8881 ttgaaagcgg cgtgacggca gatgttatcc atcatccccg gcatccgtat accattggtt
     8941 tgctccaatg cgcaccagaa catggagtac cacgccagct attacccgcc attccaggga
     9001 cggtaccaaa cctcacccat ttgcctgaag gctgcgcttt tcgcgatcgt tgctatgcgg
     9061 caggtgcaca gtgtgaaaac gtcccggcgc tgacagcgtg tggtgacaac aaccagcgct
     9121 gcgcctgttg gtatccgcag caggaggtca taagtgtctg acacgttatt aacgttacgc
     9181 gacgtccata tcaatttccc ggcccgtaaa aactggcttg gtaaaactac ggaacatgtt
     9241 catgccatta atggtattga tttacagatc cgccgtggtg aaaccttagg gatcgtcggc
     9301 gagtcaggct gcggcaaaag caccctcgca cagcttttaa tgggcatgct gcaaccgagc
     9361 cacgggcagt acatccgttc tggctcacaa cgcattatgc agatggtgtt tcaggacccg
     9421 ctctcttcgc tcaatccgcg cttaccggtg tggcgcatca tcacagaacc gctctggata
     9481 gctaagcgta gtagtgaaca acagcggcga gcgttggcag aggagctggc tgtgcaggtg
     9541 ggtattcgtc cggagtatct cgaccgcctg cctcatgcgt tctccggcgg gcagcggcaa
     9601 cgcatcgcca ttgccagagc actctcttcg cagcctgacg tgattgtgct tgatgagcca
     9661 acctcggcgc tggatatctc cgtgcaggcg cagatcctca atttactggt aacgctacag
     9721 gaaaatcacg ggctgactta tgtgctgatt tcacacaatg tctcggtgat acgtcatatg
     9781 agcgatcggg tggcggtgat gtatctcggg cagattgtgg aactagggga agcgcaacag
     9841 gtgttgactg ctccggcaca tccatatacc cgattattgc tggattccct ccccgccatt
     9901 gataaaccgc tggaggaaga atgggcatta cgtaaaacgg atctgccggg aaaccgcacg
     9961 ttgccgcaag gctgtttttt ctacgaacgt tgccctctgg caacccacgg atgtgaagtc
    10021 cggcaatcat taacaataag ggaggacgga cgtgagatcc ggtgctggcg ggcgctgtag
    10081 agatgctaac aacgcatcag gcattcacgc gcctgacgcg tcatccggca atgctttacg
    10141 atttcaactg gtaatcaagc gtaatttccg ctttcagtac ctgagaaacc gggcatcctg
    10201 cttttgcttt ctggatgatg ccgtcaaagg tagaggcatc aatacccggc accgcaactt
    10261 cactcttcag tgcgattttc gtaatcgcaa aaccggcatc cactttatcc agcgacacat
    10321 cggcggtggt atcaatcgat gttggcgtaa atcccgcttc ccccagcatt aatgaaagcg
    10381 ccattgagaa acatgcggca tgcgctgcgc caatcagttc ttcagggtta gttccttttt
    10441 cgccttcaaa acgcgtgtta aatccatacg gctgttggtt cagcacacca ctctcggtgg
    10501 atactgttcc cttcccgcgt ttgatatcgc cttcccagtg tgcctgacct ttcttatgga
    10561 ttgtcattgt tgctctcctg tgggcttaaa aaggaaatta tagactacgt gagaaacagc
    10621 acgaggataa gcaggatatt ccgaataaaa atccggcaga aatattactt ctgcacttaa
    10681 attaaatttc cacaatagat aaccgggaat ata
//
LOCUS       DAZFQD010000163        10709 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.163, whole genome shotgun sequence.
ACCESSION   DAZFQD010000163 DAZFQD010000000
VERSION     DAZFQD010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10709
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.163"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..197)
                     /locus_tag="ACHKTW_004441"
     CDS             complement(<1..197)
                     /locus_tag="ACHKTW_004441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6465350.1"
                     /translation="MKIKQHKEKLEIIRDIIRETLLGNAALIVLAAMVPVVLVKPWDS
                     MTFIFMGAVCVSFAVILFWLV"
     gene            457..1278
                     /locus_tag="ACHKTW_004442"
     CDS             457..1278
                     /locus_tag="ACHKTW_004442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HFO6465351.1"
                     /translation="MKPNSTGIAARMMLSIDRARISEALLNNRQLQSTPLQIRYPQGV
                     REALGIMSEQLSLSVSDLTRILVEDALTEMFLPAENIVHRLICRTEHIMQAHDVCVTT
                     MTTLLAPWNIRPAVFRDPDKLADYLTGEILTALADWFYLNPEWLNGRVRYPLSRAGEW
                     PATQDAFCQLISASDNVDIILWHSFPFAGVHSQEYCGVLFRKKKEINKAIIYPVLSLS
                     PVRMNKEKENWFHTARNLYAKTSVRTVTLRPAQAEYLMTGKILPAELFSIPLLPW"
     gene            1368..2570
                     /locus_tag="ACHKTW_004443"
     CDS             1368..2570
                     /locus_tag="ACHKTW_004443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001532112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
                     /protein_id="HFO6465352.1"
                     /translation="MMMNMHITRTLIASAVLFSFNSTAATSYFEARNDAMGGTGVASS
                     HYGVAPLANPALLTKAGPEDDFSLLLPSVGGQLSDPGNISDNADKISDDWKAFDRAID
                     SQSNVSQTAARLKTRLQDFRHTHADAQVGTSAVATLPGDTLAAALMLKSYGTISVDGK
                     VSDADLNYLESIANSNGQDVDKDRLTSQAFARAALVTDVGIALATELETAGQKWSLGF
                     TPKFQRVDLFNYNVLVKNYDSSAFKGDRYHNTRNGINADIGASMDLDDNWTLGLVAQN
                     LLPRSIETKEVNGVTETFRIRPQVTAGVSWHNDMFTTALDVDLTPASGFTSDSKRQFA
                     AIGAEFNAWKWAQLRAGYRQNLASNDGSAFTAGLGISPFDVVHIDVAGIAGTDNNYGA
                     VAQLQFTF"
     gene            2710..2880
                     /locus_tag="ACHKTW_004444"
     CDS             2710..2880
                     /locus_tag="ACHKTW_004444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001597011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6465353.1"
                     /translation="MFNKNKTHIENKAFMVEPFRDPTANECNVVIANDICIRVALSPS
                     EVLQPFSKNTNQ"
     gene            2929..3381
                     /locus_tag="ACHKTW_004445"
     CDS             2929..3381
                     /locus_tag="ACHKTW_004445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081199.1"
                     /note="Members of this family include DotD of type IVB
                     secretion systems and TraH of plasmid conjugative plasmid
                     systems, both lipoproteins; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotD/TraH family lipoprotein"
                     /protein_id="HFO6465354.1"
                     /translation="MNRMIPAGCLAVLCLNGCHLPPDNTAPTRKYGAASDVAVARHAQ
                     YQLWGQGIYSAALPERQSGGSLKAHSDRVSFDWEGDALELLNELARLRGQQFSYSGVR
                     LPLPVSLHVRDMTFADVLRITEAQTAWRATLSQSAGRLHLSFMTPEKK"
     gene            3378..4214
                     /locus_tag="ACHKTW_004446"
     CDS             3378..4214
                     /locus_tag="ACHKTW_004446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000848138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HFO6465355.1"
                     /translation="MKTHLCLLLSALLLTGCAGQHHRSSAVMTENAPPPDIRAWLTPS
                     GNQDDGISDTRRQMLTDAGRTLGFRGGKAQRSWELIQALNARESTLNALYDFRPLISK
                     EGWLPPVIDEAQDVAHIRPDQIRTASRVWTILRPERFVSNPPGWRDWLLKGLSTTATP
                     GTEGRVVPEDRAQRRLWENALRQGWQEGRLNADLTLEANQKRLTRDYRGMMLYALLWR
                     QGMITRPDVTEQMQTVTGNGQKLITGDRVRRLKTHAEFTLQKSRWRPLVSTEGEKNDI
                     TR"
     gene            4198..5346
                     /gene="traJ"
                     /locus_tag="ACHKTW_004447"
     CDS             4198..5346
                     /gene="traJ"
                     /locus_tag="ACHKTW_004447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid transfer ATPase TraJ"
                     /protein_id="HFO6465356.1"
                     /translation="MISPDDFGTFPFSRFTAEEFRHFFAWCARHRVSDIDLTGGSPVS
                     VSRFGRRVRSSVNPLPSSIMMALSDELFGREVLPRVLSGHPVDRTIQVSGDASGRYGL
                     QRGQRVRLRCHLIQGTSATEEKAISVTMRVIPSDIPDILTMNIEPDLLDAMLRKTGLG
                     FICGETGSGKSTLAAALYRHILKNFPDRKIVTYEDPVEYILGGENDLLPPHQAEIGRD
                     VESFAAGLRSAVRRNPDIIGIGEIRDYETADAAVQAGNTGHFCIGTMHTKSPGETFAR
                     LLGLFPPEIRDSMAAATLSLVQFILVQVLVRTNDGGRQAVREYIVITDELRNTLSGQS
                     HATWGHYIDEIIRQEKRRIRDQVLTMYQNDRIEKSEAALFIPAGEFPR"
     gene            5343..5633
                     /locus_tag="ACHKTW_004448"
     CDS             5343..5633
                     /locus_tag="ACHKTW_004448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000817799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFO6465357.1"
                     /translation="MKREGFWRDVTPPLNIYGVPLPFTMIYLLLFPYPSKTTFWVCSG
                     ILLFFMLLGYSGWTVRTLCIRLYSLLQGKQASGRPWWYRHRTELPREWHGPE"
     gene            5697..9491
                     /locus_tag="ACHKTW_004449"
     CDS             5697..9491
                     /locus_tag="ACHKTW_004449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000842452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPD7 domain-containing protein"
                     /protein_id="HFO6465358.1"
                     /translation="MKSRYRTWLAVPPEETEAVKNAVPPLNGRKAVAWDPEKKLWYAR
                     AGTELSLLERWLPRPQELSMDAGDPVTEFAQVLENAGLVIQGLPQMDGAIHRVATRDD
                     KKGAKSGAYKAYLDGRPAGWYRDYRSADDSPTNWVFSGGEQHDPLARLHLRAFAQQQR
                     DDNARKLQQQYNKQARYARSYINQLPQATEHEYLTRKGILAAPGVRLNNKNELVIPFS
                     NSRGEIRSYQRIPVTGGKDARILKDSEKTGNWFTFGTPENGRPLLFAEGYATAASLHE
                     ATGLPVLMTVDAGNMIDVAENARQIWTDSPFVFCADNDHQRKINKGVSSAQKAAASTH
                     GEVIIPAFTEAEKAQGLTDFNDLDASRGRDAFQRAMNAQLEYIGIPTPNNDTTDRREA
                     IVIGNLIFTPVKDEKPQVYPENRQSTAPEPAVSEQNHPYDIPAEETDNQAYEDDYAAY
                     ASLQSVTAGTASPNEPQVNAVSAIDSNAKNTSAATTDDRNVLAQEPNADATVTTTAHD
                     TGTAETDSPSEPAEIQPVQQASPEPSAKDKPQENVVSATDNDAENTSSATTALRSYDT
                     PETSVSDATGTQHTATTQPEEPQQVSMGSADDWSAFAEDLNADVTVMTTAHHTDTAET
                     ASPSEPAEIQPVQQATPEPTAEDKPKEERPVSATETSTANETARPETASLFSRLRRFL
                     TGGKSRTAEPPVIQENKLTEAETATEAAAPPSPPETSPEDTSSAEPDTIIFAPRRPNS
                     PQRHNLDEIIKNLTYDELPDNTALCRVDGEPAFLDKLNRLEMVDGASTDDKKVLAALA
                     VATNFYGGVIELTGSDAFKQKAMQLIVEYDIKVRMKLPVQRAALEKLRQEKGMAHDAI
                     VTHRPTPELNRLSPEETTMAQPAPPVSPKAETEQTAGSTAETPHPEAPVTAKAEAPRA
                     GTPEQPAAPSSSAGNVAVSAQPQQPDTPEEEPPGKLRPGESVTAVLTNYGEKEYAPGK
                     GRCFFVELTNRSGKREYWGNGLEELVKNHQKGNPVTLTLQARETFRLSGETRDRIRNI
                     WAMTPVSNGITVDNDHPHAGQHILDFPMATFSQVMLQMQQHWPQIMQEVRSPKNPPNN
                     LYIGEDRQPASAPQDQSMRLPLAGNAPNELIPAIASADKDNRQLNLLLVHSADDHLQG
                     VVRLNGTLYPALATPSADNRQLVINALTDNGLQFAGYGEAVNHDENNHQRPSPQIMQF
                     HLKQQDSPLFAAIHKPEEQPDKLFRSLGFEQTWKEWSDSQKAEDRQEKTLQQAQSHSP
                     GR"
     gene            9508..9858
                     /locus_tag="ACHKTW_004450"
     CDS             9508..9858
                     /locus_tag="ACHKTW_004450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFO6465359.1"
                     /translation="MNMKKTLLVILTGITLASPVAGAATCRAGAAAEAGSKAAWERAR
                     TASQAWSQRENDVASSLQSCLSRIKDVSITLPQFPSLDKILRDLENQVCDAVVDRVNE
                     HLPGNIDPWKDYNL"
     gene            9870..10565
                     /locus_tag="ACHKTW_004451"
     CDS             9870..10565
                     /locus_tag="ACHKTW_004451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000138553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotI/IcmL family type IV secretion protein"
                     /protein_id="HFO6465360.1"
                     /translation="MSEAHPTSPVSPETDNSGDIYKQTLSTIAQSEQRAALVPGLVRA
                     LLCSSAALVISLTGNIIQYWHNSNVTREYFATDNGRLVRLAPTSQPAWSQSDVMTFGS
                     MTLSEAFNLDFVHYRKQITTLAPRFSEAGFAGYVNALQASNILDTIKKEKFNLTATIG
                     AGTLVNQGQMENGIWFWKFQYPVRMRLVGQSSTRPEQAFTFEITVRRVDPRIKPGGME
                     ISQMVSRNAASGT"
     gene            10576..>10709
                     /locus_tag="ACHKTW_004452"
     CDS             10576..>10709
                     /locus_tag="ACHKTW_004452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001165562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraN"
                     /protein_id="HFO6465361.1"
                     /translation="MQIHHLLGALLIVCGTVTAADNPSWQNARTPQAGGKTGQAPGNA
                     P"
BASE COUNT         2783 a         3099 c         2639 g         2188 t
ORIGIN      
        1 taaaccagcc agaacagaat gactgcaaaa gaaacacaca cggcccccat aaaaataaat
       61 gtcatcgaat cccatggttt caccagtaca accggaacca tggctgccag aacaataaga
      121 gcagcattac ccaacagcgt ctctcgaata atatccctta taatctccag tttttcttta
      181 tgctgtttaa ttttcatacg aacctcgttg atttcatccc gaaaaggaaa agattatata
      241 acgaaatact ttcaatctcc tgactccgtc tgctctgcag cataatgcca gaccagcggc
      301 atgctaaatc acgtcctgtc cgaatccttt tcattcacgc ttaaaacaga gttaaaagca
      361 ctcttgaaac acactcgatc ataacgatcg aaataactcg tttccgttca gtaaacacgc
      421 tctttagact taacatatga accgcacgga ggtgaaatga aacccaacag cacaggcatt
      481 gctgcccgca tgatgctctc cattgacagg gcccggatta gcgaagctct gctcaataac
      541 cgccagttgc agtccactcc cctgcagatc cgttatccgc agggcgtcag agaagcactc
      601 ggaataatga gcgagcaact ctccctttct gtatccgacc tgacgcgtat cctggtcgaa
      661 gatgcattaa ctgaaatgtt tctcccggct gaaaacatcg ttcaccgtct tatctgccgg
      721 acagaacaca tcatgcaggc acatgatgtc tgcgtaacaa cgatgacaac gttactggct
      781 ccctggaata tccgtcctgc cgttttccgt gacccggaca aactcgcaga ttatctgaca
      841 ggagagattc tgacggctct ggctgactgg ttttatctca acccggaatg gctgaacggc
      901 agggtacgtt atcctttaag ccgtgccggt gagtggccag caacacagga tgcgttctgc
      961 cagcttattt cagccagcga taatgtggat attattctct ggcacagttt tccttttgcc
     1021 ggtgttcatt cacaggaata ttgcggtgta ctgttccgca agaaaaaaga aattaataaa
     1081 gccatcattt atcctgtact ttctttatca cctgtaagaa tgaataagga aaaagaaaac
     1141 tggtttcaca ctgcccgcaa tttatatgca aagacatccg tccggaccgt aacgcttcgt
     1201 ccggcacagg cagaatatct gatgacagga aaaatattac ctgctgagtt attcagtata
     1261 ccgttattac cctggtaata acgcagagaa aaaaccaata aatttttaat ggcacagtcg
     1321 tgtcataccg ttgctgtacc caaaacacct caaaccagga aactgaaatg atgatgaata
     1381 tgcacattac ccgaactctc atcgcatctg ctgttttatt ttcctttaat tcaacagccg
     1441 caacttcata ttttgaagcc cgcaatgatg caatgggggg aaccggggta gcctcctctc
     1501 actacggtgt ggcacctctg gcaaacccgg ccctgctgac aaaagccggt ccggaagatg
     1561 acttcagtct tctgcttccg tccgttggtg ggcaattgtc cgatccgggt aatatcagtg
     1621 acaacgcaga caaaatcagt gatgactgga aagcctttga tcgtgccatc gacagtcaga
     1681 gcaacgtgtc gcagactgct gcacgtctga agacgcgact tcaggacttc cgtcacacac
     1741 atgctgacgc acaggtggga acatctgctg tggcgactct tcccggtgac accctcgctg
     1801 ccgctctgat gctgaaaagc tacggtacga tcagtgtcga cggtaaagtc agtgatgccg
     1861 acctgaacta tctggaaagc attgcaaaca gtaacggtca ggatgtggat aaagacagac
     1921 tgacctctca ggcttttgcc cgtgccgcac tggtcaccga tgtgggtatt gctctggcta
     1981 ctgaactgga aacggccgga caaaagtggt ctctgggctt cacaccaaaa ttccagcgtg
     2041 ttgacctgtt taactacaac gtgctggtta aaaattacga cagcagcgct ttcaaaggcg
     2101 accgttacca caacaccaga aacggcatta acgctgatat cggggccagc atggacctgg
     2161 atgacaactg gacactgggt ctggtcgcac agaacctgct cccccgcagt attgagacaa
     2221 aggaagtgaa cggtgtcaca gaaactttca ggatccgccc gcaggtgact gccggtgtct
     2281 cctggcacaa cgacatgttc actacagcac tggatgttga cctgaccccg gccagtggct
     2341 tcacatcaga cagtaaacgt cagtttgctg ccattggtgc tgaatttaat gcctggaaat
     2401 gggcacagtt acgtgcaggc taccgccaga atctggccag caatgatggc agtgcgttca
     2461 ccgccgggct gggtatctca ccatttgatg tcgtccatat tgatgtggcg ggtatagcag
     2521 gcacagacaa caactacggt gccgttgcac aactgcagtt cacattctga tcatctctgc
     2581 cccggtactg accggggtat ccagacctgc atcctcgcgt tttccgacca tcaaacgaca
     2641 cagccagacc tgtagtgctg cctctgatct ggctgttatc tgacatatca ttttcgcatg
     2701 aggaaatgca tgtttaacaa aaataaaacg catatcgaaa acaaagcctt tatggtggaa
     2761 ccgttccggg atccgacagc taacgaatgt aatgtcgtga tagcaaacga tatttgtatc
     2821 cgggtagcac tgtctccctc tgaggtgctc cagccattca gcaaaaacac gaaccagtaa
     2881 atcctcccca caacgctttc ttctttcact gctcaggaaa aatacaccat gaaccgtatg
     2941 atacctgcgg gatgtctcgc cgtactctgc ctgaacggct gccatctccc cccggataat
     3001 acagctccga ccagaaagta tggtgctgcc tctgatgtcg ccgtcgcccg gcatgcgcag
     3061 tatcagctat gggggcaggg aatttactct gcagcccttc cggagcgtca gtccggcggc
     3121 agtctgaagg ctcacagcga ccgtgtttcc tttgactggg aaggcgatgc cctggaattg
     3181 ctcaacgaac tggcccgtct gcgcggccag cagttcagct acagcggcgt ccgtctcccc
     3241 ttacccgtct cactgcacgt ccgcgacatg acctttgctg atgtccttcg tatcactgaa
     3301 gcccagactg cctggcgggc aacactctcg cagtcagccg ggcgtttaca tctgtctttt
     3361 atgacgccgg agaaaaaatg aaaacacacc tgtgtctcct gctgtctgct ctcctgctta
     3421 caggctgtgc cggacagcat caccgttctt ctgccgtcat gacagaaaac gcaccgcccc
     3481 cggatatccg tgcatggctg acgccctccg gaaaccagga tgatggtatt tcagataccc
     3541 gtcgtcagat gctcacggat gccggcagaa cgctgggatt ccgtggtgga aaagcacagc
     3601 gctcatggga actgattcag gcactgaatg cccgggaaag taccctgaat gccctgtatg
     3661 atttccggcc tctcatcagc aaggaaggct ggctgcctcc tgttatcgat gaagcgcagg
     3721 atgttgcaca catcagaccg gaccagataa gaacagcctc ccgtgtctgg accatcctcc
     3781 gcccggaacg ctttgtcagt aatccacctg gatggcgtga ctggctgctg aaaggactct
     3841 caaccaccgc cacaccaggc actgaaggcc gggttgtgcc ggaagaccgg gcacaacgca
     3901 gactatggga aaacgcctta cgtcagggat ggcaggaagg gcgcctcaac gcagatctca
     3961 cactggaagc taatcagaag cgcctgaccc gtgattaccg gggcatgatg ctttacgccc
     4021 ttctgtggcg tcagggcatg atcacacgtc cagatgtcac agagcagatg cagacggtta
     4081 ccggcaacgg gcaaaaactc atcactggcg atcgcgtccg ccgcctgaaa acacatgctg
     4141 aatttacgct gcagaaatcg cgctggcgcc ctcttgtcag caccgaagga gagaaaaatg
     4201 atatcaccag atgacttcgg taccttcccc ttctcccgtt ttacggcaga agagtttcgc
     4261 catttttttg cctggtgtgc ccgccaccgc gtcagtgata ttgatctgac cggcggttca
     4321 ccggtatccg tcagtcgctt tggacggcgg gttcgctcat ccgtaaatcc ccttccgtct
     4381 tccatcatga tggcactgag tgatgagctg tttgggcggg aggttcttcc ccgggttctg
     4441 tcaggtcatc ccgtggatcg taccattcag gtcagcggag atgcctccgg ccgctatggc
     4501 ctgcagcgag gtcagcgtgt tcgcctgcgc tgccacctga tacagggaac atcggcaaca
     4561 gaagaaaaag ccatttccgt caccatgcgg gtgatcccgt cagatattcc ggacatcctc
     4621 actatgaata ttgaaccgga tctgctggat gccatgctca gaaaaaccgg cctgggcttc
     4681 atctgcggag aaaccggctc cgggaaatcc acactggcgg cggcactgta tcgccacatt
     4741 ctgaaaaatt tccccgatcg caaaatagtg acctatgaag atccggtgga atacattctg
     4801 ggcggagaga atgaccttct gcctccccat caggccgaaa tcgggcgcga tgtggaaagt
     4861 tttgctgcag gacttcgctc tgccgttcgc cgtaatccgg acatcattgg tatcggtgaa
     4921 atcagggatt atgaaacagc agatgccgca gtccaggcgg gtaataccgg acacttttgt
     4981 atcggcacca tgcacacaaa atcacccgga gaaacctttg cccgcctgct gggattgttt
     5041 cctcctgaaa tcagggattc gatggctgca gccacgcttt ccctcgtaca gttcattctg
     5101 gtgcaggtac tggtcagaac aaatgacggc ggcaggcagg ctgtccggga atacatcgtc
     5161 ataactgacg aactgcgcaa tacgctctcc ggacaatccc atgcaacatg gggacattac
     5221 attgacgaaa tcattcgtca ggaaaaacgg cggataaggg atcaggttct gaccatgtat
     5281 cagaacgacc gcattgagaa atcagaagca gcgctcttca tcccggccgg agagttcccc
     5341 cgatgaaacg cgaaggcttc tggcgggatg taacaccacc gctcaacatt tatggtgtcc
     5401 ccctgccgtt taccatgatt tacctgctgt tgtttccgta tccgtcaaaa acgacattct
     5461 gggtgtgcag cggcattctg ctgtttttta tgctcctggg atattccggc tggacagtcc
     5521 gcaccctctg tatacgtctg tacagcctgc ttcagggtaa gcaggcatcg ggacgtccct
     5581 ggtggtaccg acaccgcaca gaattacccc gtgaatggca cggaccagaa tagccctctt
     5641 ttgtccatca tcccggcgta tcacaacgcc ctcatattta atccccggag aacgacatga
     5701 agtccagata tcgtacctgg cttgctgtcc cgcctgagga gacagaagcc gtaaaaaatg
     5761 cagtcccccc gttaaatggc cgcaaagctg tcgcatggga tccagaaaaa aaactctggt
     5821 atgcccgggc gggtaccgaa ctttcactgc tggagcgctg gttaccccgg ccgcaggaac
     5881 tgtcgatgga tgccggcgat ccggtcaccg aatttgctca ggtcctggaa aacgccggac
     5941 tggttattca gggattgcca cagatggatg gcgccataca cagagtcgcc acccgggatg
     6001 acaaaaaagg ggccaaaagt ggcgcataca aggcctatct tgatgggcgc cccgccggct
     6061 ggtaccgcga ctaccggagc gccgatgaca gtcccacaaa ctgggtattt tccggcggag
     6121 aacaacacga tcctctggcc agactgcatc tgcgggcatt cgctcagcaa cagcgcgatg
     6181 ataacgcacg taaactgcag caacagtaca acaaacaggc ccggtatgcc cgctcctata
     6241 ttaaccagtt accacaggca acagagcatg aatacctgac ccgaaaaggg atccttgccg
     6301 caccgggcgt ccgtctgaat aacaagaatg aactcgtcat ccctttcagt aacagccggg
     6361 gagaaatacg ttcataccag cgtattcctg tcaccggcgg gaaggatgcc cgcattctga
     6421 aagactcaga gaaaaccggt aactggttca ccttcggaac cccggaaaat ggccgtcccc
     6481 tgttatttgc ggaaggttac gcaactgcag cctccctgca cgaagccacc ggccttccgg
     6541 ttctgatgac agttgatgcc ggcaatatga ttgacgtggc agaaaatgcc cgccaaatct
     6601 ggactgacag cccctttgtc ttctgtgccg acaacgacca tcaacggaaa attaacaaag
     6661 gtgtttcaag tgcccaaaaa gctgccgcaa gcacgcatgg cgaagtcatt attccggcct
     6721 ttacggaggc ggaaaaagca cagggactga ccgatttcaa tgacctggac gccagcaggg
     6781 gacgagacgc ctttcagcga gccatgaacg cacagttaga atatatcggt atccccacgc
     6841 ccaacaatga cactactgac cgccgtgaag ctatcgtgat cggcaacctg atattcacac
     6901 cggtaaaaga tgagaagcca caagtgtacc ctgaaaacag acagagcaca gcccctgaac
     6961 cggcagtgtc ggaacagaat catccttatg acatccctgc agaagagaca gacaatcagg
     7021 catatgaaga tgactatgca gcatatgcat ccctgcagtc ggtaactgca gggacagcct
     7081 ccccgaatga accgcaggtg aatgctgttt ctgccattga cagcaacgca aagaatacct
     7141 ctgccgccac cactgacgac cgaaatgtat tagcacaaga acccaatgct gacgccacag
     7201 ttacgaccac ggctcacgac accggcacag cagaaacaga ttcaccttct gaacctgcag
     7261 agattcagcc agtgcagcag gccagtcctg aaccatcggc taaagataaa ccacaggaga
     7321 acgtagtgtc tgctactgat aatgacgcag agaacacctc ttccgccacc actgccctcc
     7381 ggagttacga cacaccggaa acatccgtct ccgacgcaac cgggacacag cacaccgcca
     7441 caacgcaacc tgaagaacca cagcaggtct ccatgggcag cgctgacgac tggagcgcat
     7501 ttgcggaaga cctcaatgct gatgttactg tgatgaccac agctcaccac accgatacag
     7561 cagaaacagc ctcaccttct gaacctgcag aaattcagcc cgtacagcag gcaacccctg
     7621 aaccaacggc agaagataaa ccgaaggaag agcgtccagt gtctgccact gagaccagca
     7681 cagcgaatga aacagcccgc cctgaaacag catcgctttt cagccgcctg cgccgttttc
     7741 tgaccggtgg aaaatcacgt accgccgaac ctcccgttat tcaggagaac aagcttacgg
     7801 aggccgaaac tgcgacagag gccgcagcac caccgtcacc acctgaaacc agcccggaag
     7861 acacatcgtc agcagaaccg gacacgatta tctttgcgcc ccgccggccc aacagtccgc
     7921 agcggcacaa cctggacgag attattaaaa atcttacata cgatgagtta cccgacaata
     7981 ctgcgctctg ccgcgtcgac ggcgaaccgg cattcctgga taaactcaac cgtctggaaa
     8041 tggtggatgg tgccagcact gatgacaaaa aggtcctggc tgcccttgct gtggccacca
     8101 acttctatgg cggcgttatc gagctgaccg gcagtgatgc ctttaaacaa aaagccatgc
     8161 agctgattgt cgaatatgac atcaaggttc gcatgaaact cccggtccag agggctgcgc
     8221 tggaaaaact ccgccaggag aaagggatgg cgcatgatgc catcgtcacc cacagaccca
     8281 cgccggaact caaccgcctg tcaccggaag agacaacaat ggcacaaccg gccccccctg
     8341 tgtcaccgaa agcagaaaca gagcaaactg caggcagtac ggctgaaaca ccgcatccgg
     8401 aagcgccggt cacggcaaaa gctgaagccc cccgggccgg cacaccggaa caaccagccg
     8461 ccccgtcatc gtcagcagga aacgtcgctg tcagtgcaca gccacagcaa cccgacacac
     8521 cggaggaaga gccccccggc aaactcagac ccggtgaaag tgtgactgcc gtcctgacaa
     8581 actacggaga aaaagagtat gcgcccggaa aagggcgctg cttttttgtg gagctcacca
     8641 accgcagtgg caaacgggaa tactggggaa acggcctgga agaactggtc aaaaaccacc
     8701 agaaaggcaa ccccgtcaca ctgacgctgc aggcgagaga aacattccgg ctgtccggtg
     8761 aaacacggga tcgtatccgt aatatctggg ccatgactcc tgtcagtaac ggcattaccg
     8821 tcgataatga ccatcctcat gctggtcagc atatcctgga ttttccgatg gcaacattca
     8881 gccaggtgat gttacagatg caacaacact ggccacagat aatgcaggaa gttcgctccc
     8941 ccaaaaatcc tcccaataat ctgtacattg gtgaagacag gcaacctgca tcagcacctc
     9001 aggatcaatc aatgcgactc cccctcgctg gtaatgcccc caatgaactg ataccagcca
     9061 tcgcctctgc cgataaggac aatcgtcagt tgaatcttct gctggtccac agcgcagatg
     9121 atcatctgca gggcgtggtc cgcctgaacg gcacccttta cccggcactg gccaccccgt
     9181 cagctgacaa ccgtcagctg gtcatcaatg ccctgacgga taacggtctg cagtttgccg
     9241 gttacggtga agctgttaac catgatgaga ataatcatca gcgtccgtcc ccacaaataa
     9301 tgcagtttca cctgaagcaa caggactccc cgttgtttgc cgccatccac aaaccggaag
     9361 agcaaccgga caagcttttc cgctctctgg gttttgagca gacctggaaa gagtggtctg
     9421 acagccaaaa ggcggaggac aggcaggaaa aaactctgca acaggcacaa tcccacagcc
     9481 ccggccgatg agccggtaag gacgaccatg aacatgaaaa aaacgctgct ggtcatactg
     9541 accggcatca ccctggcatc accggtggca ggtgccgcca cctgccgggc gggggccgct
     9601 gccgaagccg gcagcaaagc agcctgggag cgggcgcgca cagccagcca ggcatggtca
     9661 cagcgagaaa acgacgtcgc ctcctccctg cagtcctgcc tgtcccgcat taaggacgtc
     9721 agcatcactc ttccgcaatt ccccagtctg gacaaaatcc ttcgcgatct ggaaaaccag
     9781 gtctgtgatg ccgtcgtgga tcgggtgaat gaacatctgc cggggaatat cgacccctgg
     9841 aaagactata acctctgaac aggagcccca tgagcgaagc acaccccaca tcccctgtct
     9901 ctccggaaac agataacagc ggagatattt ataaacaaac gctcagtacc attgcacagt
     9961 cagaacaacg ggccgcactg gttcccggac ttgtcagggc cttgctgtgt agcagcgcag
    10021 ctctcgtcat cagtctgacc ggcaatatca tacagtactg gcataacagc aacgtaacgc
    10081 gtgaatactt tgccaccgac aatggccggc tggtccgcct ggcccccacc agtcagccag
    10141 cctggagtca gagcgacgtc atgacgtttg gcagcatgac gctgagcgaa gcttttaatc
    10201 tggattttgt ccactacagg aaacaaatca cgacactggc cccccgcttt tccgaagccg
    10261 gttttgccgg ctatgtgaat gctcttcagg cctccaacat tctggacacc attaaaaagg
    10321 agaaatttaa cctcacggcc accatcgggg ccggcacgct ggtgaatcag ggccagatgg
    10381 aaaacggcat ctggttctgg aaattccagt atccggtcag gatgcgtctg gtcggtcaga
    10441 gcagcacccg ccccgagcag gccttcacct ttgaaatcac tgtccggcgc gttgacccac
    10501 gaatcaaacc cggcggcatg gaaatcagcc agatggtttc ccgtaacgcc gcatcaggaa
    10561 cctgagactc atatcatgca aatacatcat cttcttggcg cactgcttat tgtgtgcggc
    10621 actgtcacgg ctgcagataa cccgagctgg cagaacgcac gaaccccaca ggctggcgga
    10681 aagacaggac aggctcccgg gaacgcccc
//
LOCUS       DAZFQD010000164        10450 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.164, whole genome shotgun sequence.
ACCESSION   DAZFQD010000164 DAZFQD010000000
VERSION     DAZFQD010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10450
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.164"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            122..697
                     /gene="ssuE"
                     /locus_tag="ACHKTW_004453"
     CDS             122..697
                     /gene="ssuE"
                     /locus_tag="ACHKTW_004453"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_process: GO:0046306 - alkanesulfonate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFO6465362.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNGLDVEVYHWNLQNFAP
                     EDLLYARFDSPALKTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALKPVLSALKAQEILHGVFADDSQVIDYHHRPQFTPNLQTR
                     LDTALETFWQALHRRDVQVPDLLSLRGNAHA"
     gene            690..1649
                     /gene="ssuA"
                     /locus_tag="ACHKTW_004454"
     CDS             690..1649
                     /gene="ssuA"
                     /locus_tag="ACHKTW_004454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HFO6465363.1"
                     /translation="MRNIIKLALVGLLSVSTIAVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQSKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLVYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     VNAEVAALQQQTADLFYENRLVPKKVDIRQRIWQPTQLEGKQL"
     gene            1646..2791
                     /gene="ssuD"
                     /locus_tag="ACHKTW_004455"
     CDS             1646..2791
                     /gene="ssuD"
                     /locus_tag="ACHKTW_004455"
                     /EC_number="1.14.14.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008726 - alkanesulfonate monooxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
                     /protein_id="HFO6465364.1"
                     /translation="MSLNMFWFLPTHGDGHYLGTEEGSRPVDHGYLQQIAQAADRLGY
                     TGVLIPTGRSCEDAWLVAASMIPVTQRLKFLVALRPSVTSPTVAARQAATLDRLSNGR
                     ALFNLVTGSDPQELAGDGVFLDHSERYEASAEFTQVWRRLLLGETVNFNGKHIHVRGA
                     KLLFPPIQQPYPPLYFGGSSDVAQELAAEQVDLYLTWGEPPELVKEKIEQVRAKAAAH
                     GRKIRFGIRLHVIVRETNDEAWQAAERLISHLDDETIAKAQAAFARTDSVGQQRMAAL
                     HNGKRDNLEISPNLWAGVGLVRGAAGTALVGDGPTVAARINEYAALGIDSFVLSGYPH
                     LEEAYRVGELLFPHLDVAIPEIPQPQPLNPQGEAVANDFIPRKVAQS"
     gene            2802..3593
                     /gene="ssuC"
                     /locus_tag="ACHKTW_004456"
     CDS             2802..3593
                     /gene="ssuC"
                     /locus_tag="ACHKTW_004456"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HFO6465365.1"
                     /translation="MATPVKKWLLRVAPWFLPVGIVAVWQLASSVGWLSTRILPSPEG
                     VVTAFWTLSASGELWQHLAISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQ
                     MLRNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEMARS
                     YGLSGIPLFIHVILPGALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREF
                     LQTDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATV"
     gene            3590..4357
                     /gene="ssuB"
                     /locus_tag="ACHKTW_004457"
     CDS             3590..4357
                     /gene="ssuB"
                     /locus_tag="ACHKTW_004457"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HFO6465366.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLRLLAGLETPTAGDVLAGTTPLAEIQEDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAVGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQEHGFTVLLVTHDVSEAVAMADRVLLIEEGKIGLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMRRGHSEQLIRRHG"
     gene            complement(4564..7176)
                     /gene="pepN"
                     /locus_tag="ACHKTW_004458"
     CDS             complement(4564..7176)
                     /gene="pepN"
                     /locus_tag="ACHKTW_004458"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HFO6465367.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDELWTAWKEEEGALVISNLPERFTLKIINEISPAA
                     NTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKIKYPFLLSNGN
                     RVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDRG
                     NLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKYV
                     LARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSDL
                     GSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMIH
                     TLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPIVTV
                     KDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVNS
                     VLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRWD
                     AAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVNE
                     MAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLRN
                     ACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKWH
                     QDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAFH
                     AEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLEN
                     LSGDLYEKITKALA"
     gene            7442..8644
                     /gene="pncB"
                     /locus_tag="ACHKTW_004459"
     CDS             7442..8644
                     /gene="pncB"
                     /locus_tag="ACHKTW_004459"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFO6465368.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQIQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            8813..10213
                     /gene="asnS"
                     /locus_tag="ACHKTW_004460"
     CDS             8813..10213
                     /gene="asnS"
                     /locus_tag="ACHKTW_004460"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFO6465369.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
BASE COUNT         2370 a         2701 c         2801 g         2578 t
ORIGIN      
        1 agtttatttt cagaaaaaga tacacaaaat ttatatttgg cattttcttg tctctccgac
       61 agactgaccg aattgttatc aatgttaaca aaaaaagaac aattggttat aaggagagag
      121 tatgcgtgtc atcaccctgg cgggtagtcc tcgctttcct tctcgctcca gctccttgct
      181 ggaatatgcg cgggaaaaac taaatggcct cgatgtagag gtttatcact ggaatctgca
      241 aaacttcgcc ccggaagatc tgctttatgc tcgtttcgat agtccggcac tcaagacctt
      301 caccgaacag ctgcaacagg ctgatggact gatagtcgct acacctgtgt ataaagccgc
      361 ctattccggc gcgctgaaaa ccctgctcga cctgctgcca gagcgcgctt tgcaaggcaa
      421 agtggtgcta ccgctggcga cgggcggtac cgtggcccat ctgctggcgg tcgattatgc
      481 ccttaaacca gttttaagcg cactgaaagc tcaggagatc ctgcacggcg tgtttgccga
      541 tgactcacaa gtaattgatt accatcacag accccagttc acgccaaatc tgcaaacccg
      601 tcttgatacc gcgctagaaa ctttctggca ggcattgcac cgccgcgatg ttcaggttcc
      661 tgaccttctg tctctgcgag gtaatgccca tgcgtaacat cattaaactg gcgctggtgg
      721 gattgcttag cgtctctacg attgcggttg ctgcagaatc ctcgcctgaa gcgttacgta
      781 taggctatca gaaaggcagt attggtatgg tactggcaaa aagccaccag ttactggaaa
      841 aacgctatcc gcaatcaaaa atctcctggg tggagttccc cgcgggtccg caaatgttgg
      901 aagcgttaaa cgttggcagt attgatctcg gcagtaccgg ggatattccg ccaatctttg
      961 cccaggctgc cggggctgat ttggtatatg tgggcgtcga gccaccgaag cccaaagccg
     1021 aagtgattct ggtggcagaa aacagcccga tcaaaaccgt agccgatctt aaaggtcaca
     1081 aagttgcctt tcagaaaggt tccagttcac acaacctttt actgcgtgca ctgcgtcagg
     1141 ccggacttaa gtttaccgat atccaaccca cttacctgac gcccgctgat gcccgcgccg
     1201 cgttccagca aggtaacgtt gacgcctggg ctatctggga tccctactac tctgctgcat
     1261 tattacaggg cggcgtgcgg gtgctgaaag acggcaccga tctcaatcaa actggatcgt
     1321 tttatctggc agctcgtccc tatgcagaaa aaaacggcgc ttttattcag ggcgtactcg
     1381 caacctttag tgaggccgat gcgttaaccc gcagccagcg cgagcaaagc atcgctttac
     1441 tggcaaaaac gatgggctta ccggcaccgg tgattgcctc ttacttagat catcgccctc
     1501 ctaccaccat caaaccggtt aacgccgagg ttgccgcctt acaacagcaa acggcagatc
     1561 tgttttatga aaatcgtctg gtgccgaaaa aagtcgatat tcgccagcgc atctggcagc
     1621 ccactcaact ggaaggaaaa caattatgag tctgaatatg ttctggtttt taccgaccca
     1681 tggtgacggg cattatcttg gaacggaaga aggttcacgc ccggttgatc acggctatct
     1741 gcaacaaatt gcgcaagcgg cggatcgtct gggctatacc ggtgtgctga tcccaacggg
     1801 gcgatcgtgt gaagatgcgt ggctggtggc ggcgtcgatg atcccggtga cgcagcggct
     1861 gaagtttctt gtcgccctgc gccccagcgt aacctcacct accgttgccg ctcgccaggc
     1921 cgccacgctt gaccgtctct caaatggtcg tgcgttgttt aatctggtca caggcagcga
     1981 tccacaagag ctggcaggcg acggcgtgtt ccttgatcat agcgagcgct acgaagcctc
     2041 ggcggaattt acccaggtct ggcggcgctt attgctgggg gaaaccgtca atttcaacgg
     2101 taaacatatt catgtgcgcg gagcaaaact gctcttcccg ccgattcaac agccttatcc
     2161 tccgctttac tttggcggat cgtcagacgt cgcccaggag ctggcggcag aacaagttga
     2221 tctctacctc acctggggcg aaccgccaga actagtaaaa gagaagatcg aacaagtgcg
     2281 ggcgaaagct gccgcgcatg gacgcaaaat tcgtttcggt attcgtctgc atgtgattgt
     2341 tcgtgaaact aacgacgaag cgtggcaggc cgccgagcgg ttaatctcgc atcttgatga
     2401 tgaaactatc gccaaagcac aggccgcatt cgcccggacg gattccgtag gacaacagcg
     2461 aatggcggcg ttacataacg gcaagcgcga caatctggag atcagcccca atttatgggc
     2521 gggcgttggc ttagtgcgcg gcgctgccgg aacggcgctg gtgggcgatg gtcctacggt
     2581 cgctgcgcga atcaacgaat acgccgcgct tggcatcgac agctttgtgc tttcgggcta
     2641 tccgcatctg gaagaagcgt atcgggtcgg cgagttgctg ttcccacatc tggatgtcgc
     2701 cattccggaa attccccagc cgcagccgct gaatccgcaa ggcgaagcgg tggcgaatga
     2761 ttttatcccc cgtaaagtcg cgcaaagcta aggaagaaat aatggcaacg ccagtgaaga
     2821 agtggttatt gcgcgttgcc ccctggtttt taccggtggg catcgtggcg gtgtggcaac
     2881 tggcctcctc agttggctgg ctgtcgacgc gtattttgcc ctcaccggaa ggggtggtga
     2941 cggcgttctg gacgctctcc gccagcggcg aactgtggca gcatctggcg atcagttcct
     3001 ggcgggcgct gattggcttt tcaattggcg gatcgctggg gttgattttg ggactgatta
     3061 gcgggctatc tcgctgggga gaacggctgc tggatacctc aattcagatg ttgcgcaacg
     3121 taccgcatct ggcgctgatc ccgctggtga ttttgtggtt tggcattgat gaatccgcaa
     3181 aaatctttct ggtggcgctc ggcacgctat tccccattta tatcaacacc tggcatggca
     3241 tccgtaatat cgatcgcggt ctggtggaaa tggcgcgtag ctatggatta tccggcatac
     3301 cgctgtttat ccatgtgatc ctgcctggtg ccctgccctc aattatggtc ggcgtgcgtt
     3361 ttgcgttggg cctgatgtgg ctgacgctga ttgttgccga aaccatttct gccaattcag
     3421 gcattggtta tctggcgatg aatgcgcggg agtttttgca aacggacgtg gtggtagtcg
     3481 ctattattct ttacgccctg ctcggcaaac ttgctgacgt cagcgcgcag ctgttagagc
     3541 gcctctggtt gcgctggaac ccggcttatc atttgaagga ggccacggta tgaatactgc
     3601 tcgtctgaac cagggcacac cattgttgct caatgcagta agcaaacatt acgcggaaaa
     3661 tatcgtcctg aaccaactgg atttacatat tccggcaggt cagtttgtgg cggtggtggg
     3721 ccgcagcggt ggtggcaaaa gtaccctgct gcgcctgctg gccgggctgg aaacgccaac
     3781 cgcaggcgat gtgttggcag gcaccacacc gctggctgaa attcaggaag acacgcgaat
     3841 gatgtttcag gatgcgcgtc tgctaccgtg gaaatcggtg attgataacg ttgggttagg
     3901 ccttaaaggc cagtggcgcg atgccgcgcg tcgggcgttg gctgcggtag gactggagaa
     3961 tcgcgccggg gaatggcctg ccgcactttc aggcgggcag aaacagcgag tggcgctggc
     4021 aagagcgttg attcatcgac cgggtttatt gttgcttgat gaaccgctcg gggcactgga
     4081 cgccttaacg cgactcgaga tgcaggattt gattgtgtca ctttggcagg agcacggctt
     4141 taccgtactg ttggtgacgc atgatgtgag cgaagcggta gcaatggctg accgggtgtt
     4201 gttaattgaa gagggaaaaa ttggtctgga tttgacagtg gatatccccc gtccgcgtcg
     4261 attagggtcg gtgcgattag cggagctgga agcggaagta ttgcaacggg taatgcggcg
     4321 tgggcattct gaacaactga ttcggcgtca tggttaatat gccggatgtg gcgcacaaaa
     4381 ccagcatttt caacggacta gtaggcctga taagacacgt caagcatcgc atcaggcagt
     4441 tgggcacggc tgccggatgc ggcgtgaacg ccttatccag cctacaaaac cagcaatttc
     4501 aacggagttg taggcctgat aagacgcgtc aagcgtcgca tcaggcattt tggctcaaaa
     4561 gtatcaagcc agtgctttgg tgatcttctc gtacagatcg ccagagagat tttccagccc
     4621 tttcaactgt tccagcgccg cgcgcatttt ctcctgacgt ttggcatcat aacgtttcag
     4681 gcgaatcagc ggttcaatca gacgtgaagc tacctgcggg ttacggctgt tgaggtcggt
     4741 aagcatttcc accaggaact ggtaaccgct gccatcttcg gcatggaacg ctgccgggtt
     4801 gctgcccgca aacgcaccaa tcaacgaacg aatacggttc gggttgctca tggtaaatga
     4861 gcgatgctgc aacagaccac gcaccgtctc cagcacattc gccgccgggc tggtggcttg
     4921 caggataaac catttatcca tcaccagacc gtcctgatgc cacttatcgt cgtactcctg
     4981 catcagcgcg tcacggcaag gcagctgtgc agcaaccgcc gcagaaagcg ctgccagcgc
     5041 atcggtcata ttgtttgctt catggtattg cttgctcacc agcacgtcag ccagatgcgt
     5101 ttcaccaaaa gcaaggaagc gcaggcaggc attacgcaga gtgcgttttg caatatcttc
     5161 atgctcaaca cggtactcgc tctggtaatt cgcgttgtaa atagccagca gctcatccgc
     5221 cagttcagtc gccagagtac gagtgagtgc ttcgcgtact tcggcaatag caatcgggtc
     5281 gatgatatcg aacagttcag ccatttcatt gacagaaggc agcgtcagga tttctgccgc
     5341 cagcgccgga tcaatcttct catcgagcag caccgcgcgg aaagcgtcag ccacatgtac
     5401 cggcagagac agcggctgcc cttgctgatg acgggcgacg ttcagcttga tgtaggttgc
     5461 cagcaaactt tgcgccgcat cccaacggga gaaatcatta cgcgcatgac gcatcaggaa
     5521 ggtcagttgc tgatcgctcc acttatattc cagtttcact ggcgcagaga attcgcacag
     5581 cagcgcaggc accggctgga agtagacatt atcaaaaaca aaggtctgtt ccgcctgggt
     5641 gacgttcagc acggaattca ccggatgacc gcctttctgc aacgggatca ctttgccttc
     5701 gttgtcatac aattcgatgg caaatggaat atgcagcggc tgtttttctg cctgatccgg
     5761 cgtggctggc gtgcgctggc tgatggtcag ggtgtactgc tcggtttccg gattgtagtc
     5821 gtctttgacg gtcacaatcg gcgtgccgga ctggctgtac caacggcgga aatgggagag
     5881 atcgacattc gacgcatctt ccatcgcctg cacaaagtcg tcgcaggttg ccgcgctgcc
     5941 atcatggcgc tcgaaataga gttgcatccc tttctggaag ttttcttcgc ccagcagggt
     6001 gtggatcatg cgaatcactt ccgcgccctt ctcgtaaacg gtcagggtgt agaagttgtt
     6061 catctcaatg accatatccg ggcggatcgg gtgcgccatc gggctggcgt cttccgcaaa
     6121 ctgcaatccg cgcatggtgc gcacattatt gatacggttt accgcgcggg aaccaaggtc
     6181 agagctgaat tcctgatcgc ggaagacggt taaaccttct ttcaggctga gctggaacca
     6241 gtcgcggcag gtcactcggt taccggtcca gttatggaaa tattcatggc cgataacgcg
     6301 ttcaatatcg agataatctt tgtcggtggc ggtgtcggtg cgggccaaca catatttgga
     6361 gttaaagata ttcagcccct tattctccat tgcgcccata ttgaagaaat ccaccgcgac
     6421 gatcatatag atgtcgaggt catactccag gccaaagcgt tcttcatccc atttcatgga
     6481 gtttttcagc gaggtcatcg cccacggcgc gcgatcaagg ttgccgcgat cgacgtacag
     6541 ctccagtgct acttcgcgac cagaacgcgt ggtaaaggta tcgcgcagta catcaaagtc
     6601 gcctgccacc agcgcaaaca ggtagcacgg tttcgggaac gggtcctgcc actgtaccca
     6661 atggcgtccg ttttcgagtt cgccttgcgc aacgcggtta ccattggaaa gcaggaaggg
     6721 atatttgatt ttatcggcaa taattttggt ggtaaaacgc gccagcacgt ccgggcggtc
     6781 gagataatac gtaatatggc ggaaaccttc ggcttcacac tgggtgcaaa gcgcatcgcc
     6841 tgactgataa agcccttcca gcgccgtatt cgccgccggg cttatttcat taatgatctt
     6901 aagcgtaaaa cgctccggca aattactgat gaccagtgcg ccctcttctt ctttccaggc
     6961 ggtccacagc tcatcattaa tatgaacaga aaccagtttg aggtcttcgc cgttgagacg
     7021 aagcggagca tctgatgcac catgacggac agcctggctg accgcggtaa cgaccgtctt
     7081 ttgcgcgtcg aggtcaaagg tcaagtcaat atcagtaatc tggtaatccg gcgcacgata
     7141 atcgtgacgg tatttggctt gtggctgttg agtcataaat aacctttagc atcttttata
     7201 gagtctggtg ttcagtctat tcctgttgcg taaatcgcgc tatgcagaat cttcatcttt
     7261 tcaggtacaa acgcctttat tgctacattt ttataacata caccgcgtaa tgccatcgac
     7321 cagaaaggtg gcatatggtg tgatcggggt tcaataaatt gcgaaacaag gtatactcca
     7381 gcagttcctg aagatgttta ttgtactaaa cgctcctgta cgaggacgct actgcgcacc
     7441 tatgacacaa ttcgcttctc ctgttctgca ctcgttgctg gatacagatg cttataagtt
     7501 gcatatgcag caagccgtgt ttcatcacta ttacgatgtg catgtcgcgg cggagtttcg
     7561 ttgccgaggt gacgatctgc tgggtattta tgccgatgct attcgtgaac agattcaggc
     7621 gatgcagcac ctgcgcctgc aggatgatga atatcagtgg ctctctgctc tgcctttctt
     7681 taaggccgac tatcttaact ggttacgcga gttccgcttt aacccggaac aagtcaccgt
     7741 atccaacgat aatggcaagc tggatattcg tttaagcggc ccgtggcgtg aagtcatcct
     7801 ctgggaagtt cctttgctgg cggttatcag tgaaatggta catcgctatc gctcaccgca
     7861 ggccgacgtt gcgcaagccc tcgacacgct ggaaagcaaa ttagtcgact tctcggcgtt
     7921 aaccgccggc cttgatatgt cgcgcttcca tcttatggat tttggcaccc gtcgccgttt
     7981 ttctcgcgaa gtacaagaaa ccatcgttaa gcgtctgcaa caggaatcct ggttcgtggg
     8041 caccagcaac tacgatctgg cgcgtcggct ttccctcacg ccgatgggaa cacaggcaca
     8101 cgaatggttc caggcacatc agcaaatcag cccggatcta gccaacagcc agcgagctgc
     8161 acttgctgcc tggctggaag aatatcccga ccaacttggc attgcattaa ccgactgcat
     8221 cactatggat gctttcctgc gtgatttcgg tgtcgagttc gccagtcgct atcagggcct
     8281 gcgtcatgac tctggcgacc cggttgaatg gggtgaaaaa gccattgcac attatgaaaa
     8341 gctgggaatt gatccacaga gtaaaactct ggttttctct gacaatctgg atttacgcaa
     8401 agcggttgag ctataccgcc acttctcttc ccgcgtgcaa ttaagttttg gtattgggac
     8461 tcgcctgacc tgcgatatcc cccaggtaaa acccctgaat attgtgatta agttggttga
     8521 gtgtaacggt aaaccggtgg cgaaactttc tgacagccct ggcaaaacta tctgccacga
     8581 taaagcgttt gttcgggcgc tgcgcaaagc gttcgacctt ccgcatatta aaaaagccag
     8641 ttaatatcat cagggagcta atcggctccc tttttttacc tttaattccg aaatctttcg
     8701 ctgcatttgc gaattctgct tgtctgattg cagatgccag gtaacatagg tatcccccca
     8761 tttacgggtg gacaagtgtt tattttttcc gactattaac agagagaata ttatgagcgt
     8821 tgtgcctgta gccgacgtac tccagggccg cgtagccgtt gacagcgaag tcaccgtacg
     8881 cggatgggta cgtacccgcc gagattcaaa agctggcatc tccttcctcg ccgtttatga
     8941 cggttcctgc tttgatcctg tacaggctgt catcaataat tctctgccca attacaatga
     9001 agacgtcctg cgtctgacca ccggctgctc ggtcattgtg acgggtaaag tcgtggcgtc
     9061 gccgggccag gggcaacaat ttgaaattca ggccagcaaa gttgaagttg ctggttgggt
     9121 tgaagatcca gacacttacc cgatggcggc aaaacgccac agcattgagt atctgcgtga
     9181 agtcgctcac ctgcgtccgc gcacaaacct gattggtgcc gtcgcgcgcg ttcgccatac
     9241 gctggcgcag gcgctgcatc gcttctttaa cgagcaggga ttcttctggg tttcaacgcc
     9301 actgattacc gcatctgata ccgaaggtgc aggcgaaatg ttccgcgttt ctacgctgga
     9361 tctggaaaac ctgccgcgta acgatcaggg caaagtggat ttcgacaaag acttctttgg
     9421 taaagagtct ttcctgaccg tatctggcca gttgaacggc gaaacctacg cttgcgcatt
     9481 atccaaaatt tataccttcg gcccgacttt ccgtgctgaa aactccaaca ccagccgtca
     9541 cctggcggaa ttctggatgc tggagccgga agtggcgttt gctaacctga acgatatcgc
     9601 gggtctggct gaagccatgc tgaaatatgt cttcaaagcg gttctcgaag aacgcgcaga
     9661 cgacatgaaa ttcttcgctg aacgcgtaga taaagatgcc gtttcacgtc tggaacgctt
     9721 cattgaggcc gatttcgcgc aggtggatta caccgacgca gtgaccattc tcgaaaactg
     9781 cggcaggaag tttgaaaacc cggtttactg gggcgtcgat ctctcttctg agcatgagcg
     9841 ttatctggcg gaagaacact ttaaagcacc ggtagtggtt aaaaactatc cgaaagatat
     9901 taaagcgttc tatatgcgcc tcaacgaaga cggtaaaacc gttgcggcta tggacgttct
     9961 ggctccgggc atcggtgaga tcattggtgg ctcccagcgt gaagaacgtc tggacgttct
    10021 ggacgagcgt atgctggaaa tgggcctgaa taaagaagat tactggtggt atcgcgatct
    10081 gcgtcgctac ggtactgttc cgcattcagg tttcggtctt ggttttgaac gcctgattgc
    10141 ttacgtaact ggcgtgcaaa acgtgcgtga tgtgattccg ttcccacgta ctccgcgcaa
    10201 cgccagcttc tgattttctt cctcaaagag ccagcgcctg ctggctcttt tttatccttt
    10261 ctgtcaagtt atctgtttgt taagtcaagc aatctatttg caaccccgcc ataacgatca
    10321 tcctgttacg gaatattaca ttgcaacatt tacgcacaaa aaataacccg cattcttatt
    10381 gcggattagt tttttcttag ctgatagcac aattttcata ctattttttg gcattctgga
    10441 tgtctgaaag
//
LOCUS       DAZFQD010000165        10298 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.165, whole genome shotgun sequence.
ACCESSION   DAZFQD010000165 DAZFQD010000000
VERSION     DAZFQD010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10298
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.165"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..298)
                     /locus_tag="ACHKTW_004461"
     CDS             complement(<1..298)
                     /locus_tag="ACHKTW_004461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6465370.1"
                     /translation="MEFKDLPVPFQEMASNVVRSQLATLDLSTVEKETIDNISGNVRR
                     AFIGLYEEKQLSDNQDLHEKYFLELMDIINKGFGLLMKKKGIRIAPLENHFTASS"
     gene            complement(310..462)
                     /locus_tag="ACHKTW_004462"
     CDS             complement(310..462)
                     /locus_tag="ACHKTW_004462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFO6465371.1"
                     /translation="MDTIELGNSESLVCGVFPNQDGTFTAMTYTRSKTFKTEAGARRW
                     LARNTY"
     gene            739..1041
                     /locus_tag="ACHKTW_004463"
     CDS             739..1041
                     /locus_tag="ACHKTW_004463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004142601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HFO6465372.1"
                     /translation="MKIISVKTLRDFWAENPDAEQPLRAWVDEAAKADWKSPADIKVQ
                     YRTASILKNRRVVFNIKGNHYRLIVAIAYQRGWVFVKFIGNHKEYDAIDADTIELE"
     gene            1044..1403
                     /locus_tag="ACHKTW_004464"
     CDS             1044..1403
                     /locus_tag="ACHKTW_004464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007374288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO6465373.1"
                     /translation="MNIKPIRTEQDYEAALRAVEPMFDNEPEIDTPEGDFFEVMCLLI
                     EEYEKKHYPIEPPSPIEAIRFRMEQQGLTVKDLEPAIGKKNRVYEVLNGTRSLTLPMI
                     RRLHNQFGIPLESLVGL"
     gene            complement(1450..1842)
                     /locus_tag="ACHKTW_004465"
     CDS             complement(1450..1842)
                     /locus_tag="ACHKTW_004465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000578360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO6465374.1"
                     /translation="MIFVKRLQQVLQELNINQSELGRRLGVKPQSVQGWLKGVMPRMD
                     KLEKLAELSQHPVHWFFMEEETLGDKMAVSSNDNQPQLTEQQRKIISLLDELPQSDAE
                     QIIRDMEQKRDFYKRKLEELLRQKNKTA"
     gene            1969..2229
                     /locus_tag="ACHKTW_004466"
     CDS             1969..2229
                     /locus_tag="ACHKTW_004466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001172789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO6465375.1"
                     /translation="MQKSTQVKILSIMSQSELGRRLGKTPQTISGWFKKRVPAEEVIP
                     ACEALDWGVTPHELRPDKYPNPTDGLPVEYQANAQAAAGVDS"
     gene            2226..2663
                     /locus_tag="ACHKTW_004467"
     CDS             2226..2663
                     /locus_tag="ACHKTW_004467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000693932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFO6465376.1"
                     /translation="MKIKHEHIRMAMNAWLLYPRVGRKKIADDIATAYFQLEMTYPPM
                     HDTSTTEGIGLNIQNIFRWLEKDTPDAVEKIQALIPAILTVLPRELRYHLSIFDTVER
                     RALLAAQEALSTAIDAHDDAVQAVYRKAYFSDGGSSGESVVVH"
     gene            2750..3760
                     /locus_tag="ACHKTW_004468"
     CDS             2750..3760
                     /locus_tag="ACHKTW_004468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1376 domain-containing protein"
                     /protein_id="HFO6465377.1"
                     /translation="MAALPYMQLYIADYLADTMHLSAEEHGAYLLLMFNYWQTGKPIP
                     KNRLAKISRLTNERWADVEPSLREFFCDNGDEWVHLRIEEDLASVREKLTKKSAAGKA
                     SVQARRSRKEAYVQTKQERDLTGVQTNVGVVFEHDANTKATNKDTDLKELNPTHNVRE
                     RESIPTSESHGAPLQTAEPEYLDGLSEPIGKFSMTTVWQPSPDFRQRAAVWGMALPEP
                     EFTPAELAAFRDYWMAEGKVFTQVQWEQKFARHVQHVRAQAKPVSKGVSHAASGGTAS
                     RAVQEIRASREQWERENGFISNGNGLEAVGAYGGGVFEPLDSEERGRTFEALDCPDWC
                     DD"
     gene            3753..4214
                     /locus_tag="ACHKTW_004469"
     CDS             3753..4214
                     /locus_tag="ACHKTW_004469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HFO6465378.1"
                     /translation="MTEQQIRLVCRQCMDRCRAGETWPPDLAEFVALISESGANPFGL
                     TVDAVMEEYRRWRNESWRYDGSDKYPWPQPVLYHICLEMRTRGIERQMTESELKRLAE
                     RLLTKWAKNVGNGMSVPPVRRQLEGPKHPPGPTPIELLKQEYERRKAAGFV"
     gene            4248..5018
                     /locus_tag="ACHKTW_004470"
     CDS             4248..5018
                     /locus_tag="ACHKTW_004470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFO6465379.1"
                     /translation="METVFDALKAMGKATSVELAARLDISREEVLNELWELKRNGVVD
                     KTGHTWFLAGEGESRVTEERPVKSEAQDMLTGEVEQKVTADMMIEFIGQDGAKTCEEL
                     AGKFGVSTRKVASTLAVVTATGRLARVNQNGKFRYCMPGDNLPAEPKAALVTESDGKA
                     FPQPAGAALPVREAATQEEIKTETVADIVQPLPSFTETQADELIFPSLRRANLALRRA
                     KSDVQKWERVCAALRELNKHRDIVRQITDSSRRVVSEK"
     gene            5034..5426
                     /locus_tag="ACHKTW_004471"
     CDS             5034..5426
                     /locus_tag="ACHKTW_004471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001141097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFO6465380.1"
                     /translation="MAKVFTPEEREEVKARIVEFVRLSGRETFRQLADKTGVSKTAIR
                     RLSGALAASGDVWLSGCGVFPSEQAYRVWRKTPEKAADPTLIRKLPDGEIRRYNRRQN
                     IICRECRQSEVMQRVLAFYRGNFQEVME"
     gene            5423..5719
                     /locus_tag="ACHKTW_004472"
     CDS             5423..5719
                     /locus_tag="ACHKTW_004472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001266128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4406 domain-containing protein"
                     /protein_id="HFO6465381.1"
                     /translation="MRVRVYIAGPMTGYENFNREAFHKAEEELKREGHTVLNPAVLPD
                     GLTQPHYMDICMAMIRCVDAIYMLNGWQRSAGAKAELALAEKLGHAVIYQEVAQ"
     gene            5716..6177
                     /locus_tag="ACHKTW_004473"
     CDS             5716..6177
                     /locus_tag="ACHKTW_004473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFO6465382.1"
                     /translation="MREVNYEALREAAQNYQSTLAWYQAIPDSPNAERDCDAALAAFK
                     RHIRHREADIIADLLDGLEEAKSQLNEQREYYEGVISDGSKRLAELEAREVQLPTRYD
                     LRYGHPINADERHVMIPKENGSWLYLIDLEHALRVAGIRIKGEEHGNKTRG"
     gene            6155..6511
                     /locus_tag="ACHKTW_004474"
     CDS             6155..6511
                     /locus_tag="ACHKTW_004474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6465383.1"
                     /translation="MEIKPEDELSNIVLFPVKEDDPRNQVNFLYEPSERPYCHHASVL
                     VDEKERQVRCKICGAVVEPFDWMLSVAKRETRLADDVRHLRQEERERRKNIEKLIQIE
                     RNAKARIRRATKSRTE"
     gene            6607..7032
                     /locus_tag="ACHKTW_004475"
     CDS             6607..7032
                     /locus_tag="ACHKTW_004475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6465384.1"
                     /translation="MTELTKEQLIEEAKLKIAIAKCHPNSGVARVEGELFKIALTSLE
                     AEPVAWTDDEELRDIEKYGFGEIFQRPSDKYEASRCVTPLYRMPPVPVVPEGLVKAVH
                     FYEQVKRENPPVETGAWKDAVDWVLKEVCQVVNIRIKGE"
     gene            7034..7212
                     /locus_tag="ACHKTW_004476"
                     /pseudo
     CDS             7034..7212
                     /locus_tag="ACHKTW_004476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305741.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            7546..7890
                     /locus_tag="ACHKTW_004477"
     CDS             7546..7890
                     /locus_tag="ACHKTW_004477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6465385.1"
                     /translation="MTTFTREQLIAHAEETIEAQRLCIPGTIDHDIIRTYKMDIAVLE
                     IALASLAAEPAGKLHEYKPVGHQRLVDELTMLVKQLTWQLRKTKPDCKLPGKAMDYLK
                     RNGLISAEDVLR"
     gene            8182..8337
                     /gene="hokD"
                     /locus_tag="ACHKTW_004478"
     CDS             8182..8337
                     /gene="hokD"
                     /locus_tag="ACHKTW_004478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFO6465386.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TVYEPEE"
     gene            8505..8783
                     /locus_tag="ACHKTW_004479"
     CDS             8505..8783
                     /locus_tag="ACHKTW_004479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6465387.1"
                     /translation="MAHDTKSHNSDNSAVFASRHGRRSHAFKSDWYQHPPCTEEQAEW
                     LIQCYRRRGYEFQKDLSLDRLHWIISVRLPYSERPPRPSRTFQQRIWR"
     gene            8785..9834
                     /locus_tag="ACHKTW_004480"
     CDS             8785..9834
                     /locus_tag="ACHKTW_004480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFO6465388.1"
                     /translation="MRVLLRPVLVPELGLVIVKPGHESMPVFQNTRVLVEPEPKSMRN
                     LPSGVVPAVRQPLAEDKSLLPFFSDERVIRAAGGAGALSDWLLRHVKSCQWPHGDYHH
                     SETVIHRYGAGSMVLCWHCDNQLRDQTSESLGQLAHQNLSAWMIDVIRHAMNGTQERE
                     LSLAELSWWAVCNQVADALPEAVLRRSLGLRAEKIRSVYRESDIIPGEQTATSILKQR
                     TKNIAPPSHARQQQNPPQEKTVVCITVDPESPESFMRRPKRRRWVNEKYTRWVKTQPC
                     ACCGQPADDPHHLIGHGQGGMGTKAHDIFTLPLCREHHNELHADPLAFEENHGSQIDL
                     IFRFLDHAFATGVLG"
     gene            9847..10206
                     /locus_tag="ACHKTW_004481"
     CDS             9847..10206
                     /locus_tag="ACHKTW_004481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFO6465389.1"
                     /translation="MLIDLVLPYPPTVNTYWRRRGSTYFISEEGKRYRRAVALIVRQQ
                     RLKLSLSGRLVIKVIAEPPDKRRRDLDNILKAPLDALTHAGVLIDDEQFDEINIVRGQ
                     PVSGGRLGVKIYKIESE"
     gene            10215..>10298
                     /locus_tag="ACHKTW_004482"
     CDS             10215..>10298
                     /locus_tag="ACHKTW_004482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HFO6465390.1"
                     /translation="MIYPEITGKSGEHLRLNTLEAVWIQGKL"
BASE COUNT         2791 a         2269 c         2799 g         2439 t
ORIGIN      
        1 tactgcttgc tgtaaaatga ttttcaaggg gagctattcg aatccctttc tttttcatta
       61 acaagccaaa tcctttatta atgatgtcca ttaattccag aaagtatttt tcatgtaaat
      121 cctggttatc agagagctgc ttctcttcgt acagcccgat aaaggctcgg cgtacgttac
      181 cggatatatt gtcgattgtt tctttttcta cggtactcag gtcaagagtc gccagttgag
      241 agcgaaccac attcgatgcc atttcctgga atggtactgg taaatcttta aattccatcg
      301 tcaacctcat cagtaagtgt ttctggctaa ccagcgacgc gcgccagctt cagttttaaa
      361 cgttttgctt ctggtatacg tcatcgcggt aaacgtgccg tcctggttgg gaaacacgcc
      421 gcataccaga gattcgctgt tgccaagctc gatagtatcc atgctgacct catttcccct
      481 taacgccggg ggagcggaac aaaaacctgc tgcatagtta ttaaagttga accctgccgt
      541 catgttctta cgcctcgggc tggctactta acccctgacc actgccgggt aactcgaagt
      601 attgccctgc gttctgtggg gcggggtggg tttatggata caatttacaa attaaaattt
      661 aactggtcaa catgtttatt attaaatttg taattgcgag ctgttgcatc agtcccaaaa
      721 tgggactact atacagttat gaagattatc tcagttaaaa cactcaggga tttttgggcg
      781 gagaatcctg atgcagaaca accgctaagg gcatgggtgg atgaggcggc aaaagctgac
      841 tggaaaagcc cagcagacat aaaggtacaa taccgaacgg ccagtatatt gaaaaaccgg
      901 cgcgtggtat tcaacataaa aggtaatcat taccgtttaa ttgttgcaat tgcgtatcag
      961 cgaggatggg tatttgttaa attcatcgga aaccacaagg aatacgatgc tattgatgct
     1021 gataccatcg aactggagta agcatgaaca tcaaacctat tcgtacagag caagattatg
     1081 aagccgcgtt gcgtgctgtt gaacccatgt tcgacaatga gcccgaaatt gacacgcctg
     1141 agggggattt ttttgaggtg atgtgtttgc taatagagga atatgaaaaa aaacattatc
     1201 ccattgagcc accatcccca attgaagcta taagatttcg catggaacag caggggctga
     1261 ctgtgaaaga tttggaaccc gcaattggga aaaaaaatcg ggtttatgag gtgttgaatg
     1321 gtaccagaag ccttacgtta ccaatgattc gccgtcttca taatcaattt ggtatccccc
     1381 tggaaagcct ggttggatta taaaatctgc tagtcatttg cctgatgctc gttccagaaa
     1441 aggaatgcat caggcagttt tgtttttctg ccgcagtaac tcttcaagtt tccgtttata
     1501 gaaatcgcgt ttttgctcca tatcacgaat gatctgctct gcgtcgcttt gaggtaactc
     1561 atctaaaagc gatatgattt ttcgttgttg ttctgtaagt tgcggttggt tgtcattact
     1621 ggatacagcc attttatcgc cgagagtttc ttcttccata aagaaccaat ggacgggatg
     1681 ttgtgagagc tctgctaatt tttccagttt atccattctt ggcatcacgc ctttcaacca
     1741 accttgcacg gattggggtt ttacaccaag acgtcttccc agctctgact ggtttatatt
     1801 caattcctgc aacacctgct gaaggcgttt tacaaagatc atcaccaccc ctcgtaaact
     1861 agttccgcga tcctacagaa aaaattgata agtggcatta caaatagaag ttgaaattta
     1921 agtttaaatt tgtaattatc ggtgtcatcg taaagttcgg agggaaacat gcaaaaaagt
     1981 actcaagtga aaatcctgtc aataatgagc caatcagaat tagggcgtcg tcttggtaaa
     2041 acaccgcaaa ccataagtgg gtggtttaaa aaacgagtgc cagcggagga ggttatacca
     2101 gcatgtgagg cgcttgactg gggagtaact ccgcatgaat tgcgccctga taaataccct
     2161 aatccaaccg atggtttacc tgttgagtat caggctaacg cacaagcagc ggcgggagtt
     2221 gattcatgaa aatcaagcat gagcacatcc gcatggcgat gaatgcgtgg ctgctttatc
     2281 cgagggtagg gcgcaaaaaa atcgctgatg atatagcgac ggcatatttt cagcttgaaa
     2341 tgacttatcc accaatgcat gacacctcta cgacagaggg tattggattg aacatacaaa
     2401 atatttttcg ctggcttgaa aaggatacgc ctgatgctgt tgaaaaaatt caggcactaa
     2461 ttccagctat cctgactgtt cttccgcgtg aactgcgtta tcacctcagt atttttgaca
     2521 ctgttgagcg ccgtgcatta ctggcggccc aggaagcgtt gagcacggca attgatgcgc
     2581 atgatgatgc agtccaggca gtttaccgga aagcgtattt cagcgacggc gggtcatccg
     2641 gcgagtctgt tgtggtgcat tgatatttat gccatacccc tgctgattct gttgattggg
     2701 gaatcacaga atatcaccag aggatggttc gtcacaagat gaggcaatta tggccgcatt
     2761 accatacatg caactgtaca tagctgatta cctggctgac accatgcatt tgtccgcaga
     2821 ggagcacggt gcgtatttgt tgctgatgtt caattactgg caaacaggga aaccaatacc
     2881 taaaaacagg ctggcaaaaa tttcccgtct gactaacgag cgatgggctg atgtggaacc
     2941 atccttgcgg gagttttttt gcgataacgg cgatgaatgg gtgcatcttc ggattgagga
     3001 agatctggca tcagtcaggg aaaaattgac caaaaaatca gccgccggaa aagcatctgt
     3061 tcaggccaga agaagcagaa aggaagcata tgttcaaaca aaacaagaga gagatttaac
     3121 aggtgttcaa acaaatgttg gtgttgtgtt tgaacatgac gcaaacacaa aagcaactaa
     3181 taaagataca gatctaaaag aattaaaccc cacacataac gtgcgtgaac gcgagagtat
     3241 tcctaccagt gagtcgcatg gtgcgccgtt gcagacagcc gaacctgaat acctggacgg
     3301 cctgagcgaa ccgatcggga aattttcgat gaccactgtc tggcagccgt cgccggattt
     3361 tcgacaacgg gcagctgtgt ggggtatggc tctgcctgag ccggaattta cacctgcaga
     3421 gcttgccgca ttccgggatt actggatggc ggaggggaag gttttcacgc aggttcagtg
     3481 ggagcagaaa tttgcccgcc acgtgcagca cgtcagggca caggcaaaac cagtcagcaa
     3541 gggggtaagc catgcagcat caggtggcac ggcatcacgg gcagttcagg aaatccgggc
     3601 atcacgcgaa cagtgggaac gtgaaaacgg atttatcagc aacggaaacg gcctggaagc
     3661 tgtgggagct tatgggggag gtgtattcga accgctggac tcagaagaac ggggccgcac
     3721 cttcgaagct ctggattgcc cagattggtg cgatgactga acagcaaatc cgtctggtct
     3781 gtcgtcagtg catggaccgc tgccgggcgg gtgaaacgtg gcccccggac ctggctgagt
     3841 ttgttgcact gatttcggag agtggggcaa atccatttgg tcttacggtg gatgcagtga
     3901 tggaagagta ccggcgctgg cgcaatgaat cctggcgata cgacgggagt gataaatacc
     3961 cgtggccaca gcctgtgctg taccacattt gcctcgagat gcgcaccaga gggattgaac
     4021 gtcagatgac tgaaagtgag ctgaaacgac ttgcagaacg actgttgacc aaatgggcaa
     4081 agaatgttgg taacggcatg agtgttccgc cagtgcgacg acaactggaa gggccaaaac
     4141 acccgccagg gccaacgcca attgagttac tgaaacagga atatgagcgc cggaaagcgg
     4201 ctggttttgt ctgaatttga gaaatgattt tgtcggagga aattttaatg gaaaccgtat
     4261 ttgacgcact gaaagcaatg ggaaaagcca catcggtaga actggccgcg cgacttgata
     4321 tcagtcgtga agaggttctc aacgagctgt gggaactcaa aagaaatggc gtcgttgata
     4381 aaactggtca cacctggttt ctggctggcg aaggtgaatc ccgggtaacc gaagagcggc
     4441 cagtaaaatc tgaagcacag gatatgctga ctggggaggt cgaacaaaaa gttaccgcag
     4501 acatgatgat tgagtttatc ggtcaggatg gtgctaaaac gtgtgaggaa ctggcgggta
     4561 agttcggcgt cagtactcgc aaggttgcct ccacgctggc ggtggtaacc gcaacggggc
     4621 ggctggcacg cgttaatcag aacggtaaat ttcgttactg catgccgggc gataatttac
     4681 cagcagagcc gaaagccgcg ctggtaacgg aaagtgatgg taaggccttt cctcagccag
     4741 caggtgctgc gttaccagtc cgggaagccg caacacagga agaaattaaa acagaaactg
     4801 tggcggacat tgtgcagccg ttgccatcgt ttaccgaaac gcaagcagat gagctgattt
     4861 ttccgtccct tcgcagggca aacctggcgc tgcgcagggc gaaaagtgat gttcagaagt
     4921 gggagcgagt ctgcgccgcg ctgcgggagc tgaacaagca ccgggatatt gttcgacaga
     4981 ttactgattc ttcccgccgt gttgtatcgg aaaagtgatt gccggaggcg cttatggcaa
     5041 aagtatttac accagaagag cgggaagaag tgaaggcgcg cattgtggaa ttcgtgcgcc
     5101 tgagcggacg agaaactttt cgacaactgg cagataaaac gggtgtcagt aagaccgcta
     5161 ttcgtcgttt atctggtgcg cttgcggcca gtggtgatgt ctggctctct ggttgcgggg
     5221 tatttccatc agagcaggcg tatcgcgtat ggcgtaagac accggagaag gctgctgacc
     5281 cgacactgat tcgaaagtta cctgacggag aaatacgtcg ttacaacaga cggcagaaca
     5341 taatttgtcg tgagtgccgc cagagcgaag ttatgcagcg tgtgctggcg ttctatcggg
     5401 gaaactttca ggaggtgatg gagtgagggt cagagtttat attgccggtc caatgacggg
     5461 atatgaaaat ttcaaccgtg aggcgtttca caaggcggaa gaggaactga aacgggaagg
     5521 gcataccgtc ttaaacccgg cagtacttcc ggacgggctg acacagccgc actacatgga
     5581 tatttgcatg gcaatgattc gttgtgtgga tgcgatttac atgctgaatg gctggcagcg
     5641 gtcagcgggc gctaaggcag agctggcact ggcggagaaa ctggggcatg cggtgattta
     5701 tcaggaggtg gctcaatgag agaggttaac tatgaggcgc ttcgtgaggc agcacaaaac
     5761 tatcagtcga cgctggcgtg gtatcaggct atcccggaca gcccaaatgc tgaacgggat
     5821 tgtgatgcgg ctcttgctgc gtttaagcgt cacatccgtc atcgggaagc ggatattatc
     5881 gctgatttgc tggatggact ggaagaagca aaatcacaac tcaacgagca gcgtgagtat
     5941 tacgaaggcg ttatctctga tgggagcaag cgtcttgctg aactggaagc gcgggaagtt
     6001 caattaccga ctcgctacga ccttcgatat ggacacccga taaatgcaga tgagcgacat
     6061 gtcatgatac ctaaagaaaa tggcagttgg ctttacctga ttgacctaga acacgcatta
     6121 cgcgtcgctg gcattcgcat caaaggagag gagcatggaa ataaaaccag aggatgagtt
     6181 aagcaatatc gttttatttc cggtaaaaga ggatgaccct cgtaatcagg ttaattttct
     6241 ttatgagcca tcggaaagac catattgtca tcacgcctct gtcctggttg acgaaaaaga
     6301 gcgtcaggtc cgctgtaaaa tctgcggtgc agttgtggag ccatttgact ggatgctctc
     6361 tgtggcgaaa agagaaacca gactggcaga tgatgtaagg cacttgcgcc aggaggagcg
     6421 ggaaaggcga aaaaatatag aaaagctaat tcagattgag cgtaacgcga aagcgcggat
     6481 acgcagggcg acaaaatcca gaactgaata attaaattta gaactgttaa aaatttaatc
     6541 cttagccgga gggatttctg caccctcaga acatcaggag gccgccagaa agagcggtaa
     6601 tgaaaaatga ctgaattaac aaaagagcaa ttaatcgaag aagccaaatt aaaaatagcg
     6661 attgcgaaat gccatcccaa ttcaggcgtg gcacgagtag agggtgagtt attcaaaatt
     6721 gcactgacat cgctggaagc agaaccggtg gcatggactg acgacgagga actgcgagat
     6781 attgaaaaat atgggttcgg tgagatattt cagcgtccgt cagataaata tgaggcttcg
     6841 cgttgtgtta ctccattgta tcgcatgccg ccagtgcctg tagtacctga aggactggtt
     6901 aaagctgtgc acttctatga acaagtaaaa cgtgagaatc cgccagttga gactggagcg
     6961 tggaaagacg ctgttgactg ggtactcaaa gaggtttgtc aggttgtaaa cattcgcatc
     7021 aaaggagaat gagatggcgt taacacaccg cgaactctgt cagattgcgt acaggttcct
     7081 taagcgcaac gggttcaagg tttgctttca tgaccgcttt gttgctgtaa ccagtactgg
     7141 agaacaacca gatgctatgg gattcagaaa ttcagcatca tgcctgatag aggcgaagtg
     7201 ttctcgtgct gacttgttgg cagatagaaa aaagcgtttc cgtaaaaatc cctctcttgg
     7261 catgggcgac tggagattct ttattagtga gccgggaatt atttcaattg aggatttacc
     7321 acctggctgg ggattacttc acgttgttaa cggaagagta cggaaagtac atgggtggcc
     7381 caagggtaat tgctgttggg gtgatcctga cgataagcca tttactggaa ataagcaggt
     7441 tgaatgcgat tacatgttgt ctgcattaag gcgcatggag ttgaggggac accttaatga
     7501 aatatatgac ggtgtaatcg ttaataagca agaaggaagc gcggcatgac cacttttacc
     7561 agagagcagt taatagctca cgcagaggag actattgaag cacagagact gtgcataccg
     7621 ggcacaatcg accatgacat catccgcaca tataagatgg atattgctgt tctggaaatc
     7681 gcactggcat cgctggcagc agagccagcc ggtaaattgc atgaatacaa accagtggga
     7741 catcagcgtc tggtcgacga gttaaccatg ctggtaaagc agttaacctg gcaactgagg
     7801 aaaacgaagc cggactgtaa attgccgggt aaggcgatgg actacctgaa gcgaaacgga
     7861 ctgataagcg cagaggatgt tttacgatga cctggcctga ggcattcaca acggtaggga
     7921 tcgcaatggc ggtggcgctg gtggtgtatt cgatttgtcg ctggggataa aaacgatttg
     7981 cggggaaagg atagttaagt agaattgctg cgggtgcttg aggctatctg tctcaggcat
     8041 gaataccaaa aggcagatag agaaaagccc cagttaacat tgcgcgtccg gcaagacgct
     8101 taacattaat ctgatgccat atctatgctc tacacacgta ggttagcctc ttacgtgccg
     8161 aaaggcaagg agaagcaggc tatgaagcag caaaaggcga tgctaatcgc ccttatcgtc
     8221 atctgtttaa ccgtcatagt gacggcactg gtaacgagga aagacctctg cgaggtacga
     8281 atccgaaccg gccagacgga ggtcgctgtc ttcacagtct acgaacctga ggagtaagag
     8341 accaggcggg ggagaaatct cccgccacct ctgatgtgtc aggcatcctc aacgcacccg
     8401 cgcttaaccc gcttcgtcgg gttttgtttt ttcctggcat tctggtttac aattcgcacg
     8461 tcagcctgaa caactggcac ctgctgcgcc agcagagaca accgatggcg cacgatacca
     8521 aatcacacaa ttctgataat tcagccgtct ttgccagcag gcacgggcgg cgctctcacg
     8581 catttaaatc tgactggtac cagcatcccc catgtactga agaacaagct gaatggttaa
     8641 ttcagtgcta ccgcaggcgc ggatacgagt ttcagaaaga cctcagcctc gaccgactgc
     8701 actggataat ctctgtgagg ctcccttact ccgagcgccc accgcgtcca tcccgcacat
     8761 tccagcaacg tatctggagg taacgtgcgg gtattacttc gacctgttct tgtgccagaa
     8821 ctcgggctgg tgatcgttaa gccgggccat gaatccatgc cggtattcca aaatacccgg
     8881 gtactggtgg agccggaacc gaaaagcatg cgtaatctgc cgtccggggt cgttcctgcc
     8941 gttcgccagc cgctggcgga ggataaatca ttactgccat ttttcagcga cgaacgagtg
     9001 attcgtgctg ctggtggcgc tggcgcattg tctgactggt tactgcgcca tgttaaatcc
     9061 tgccagtggc cacacggcga ttatcaccac agtgaaaccg tcattcaccg ttatggcgct
     9121 ggctcgatgg tgttgtgctg gcactgtgac aaccagctgc gtgaccagac atccgaatca
     9181 ctcgggcaac ttgctcatca aaacctgtca gcatggatga ttgacgtcat acgccatgca
     9241 atgaatggca cgcaggagcg ggaattgtcg ctggctgaat tatcctggtg ggcggtctgc
     9301 aatcaggtgg cggacgcgct tccggaggca gtattacgtc gttctctggg gttacgtgcg
     9361 gaaaaaatcc gctcggtgta tcgcgaaagc gacatcatac cgggagagca gaccgccacc
     9421 agcatactga aacagcgcac aaaaaatatt gcgccgccgt ctcacgcccg ccagcaacag
     9481 aacccaccac aggaaaagac ggtggtatgc atcaccgttg atccggagtc tccggaatct
     9541 ttcatgaggc gacctaaacg tcgccgttgg gtaaatgaga aatatacgcg ctgggtgaag
     9601 acacagccgt gtgcgtgttg tggtcagcca gccgacgatc cccatcacct gattggtcac
     9661 ggtcagggag ggatgggaac aaaggcccac gatattttca cgctaccgtt gtgccgggaa
     9721 catcacaacg aacttcatgc ggatccgctg gcgttcgaag aaaatcatgg ttctcagatt
     9781 gatttaattt ttcgttttct tgatcacgcc tttgcaactg gcgtgcttgg gtaaaagagg
     9841 tgactgatgc tcatagattt ggttttacct tacccgccga cggtgaacac ttactggcga
     9901 cgccgtggca gcacatattt tatctcggag gagggaaagc gttatcgccg ggctgtggcg
     9961 cttattgttc gccagcagcg gctgaaatta agcctgtccg gaaggctggt gataaaggtg
    10021 attgcagagc caccggataa gcgtcgtcgc gacctggaca acattctgaa agcaccgctg
    10081 gatgcgctga cgcatgcggg agtgctcatt gatgacgagc agtttgatga aatcaatatt
    10141 gtacgtggtc agccagtatc tggtggacgg ctgggtgtga agatttacaa aattgagagt
    10201 gagtgagcgt aaatatgata tatccggaaa ttacaggcaa aagcggcgaa catttacgcc
    10261 tgaacacgct ggaagcagtc tggatccagg ggaaatta
//
LOCUS       DAZFQD010000166        10227 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.166, whole genome shotgun sequence.
ACCESSION   DAZFQD010000166 DAZFQD010000000
VERSION     DAZFQD010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10227
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.166"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..517)
                     /locus_tag="ACHKTW_004483"
     CDS             complement(<1..517)
                     /locus_tag="ACHKTW_004483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFO6465391.1"
                     /translation="MAFRMSEQARTIKIYNLLAGTNEFIGEGDAYIPPHTGLPANSTD
                     IAPPDIPAGFVAVFNSDEASWHLVEDHRGKTVYDVASGDALFISELGSLPENVTWLSP
                     EGEFQKWNGTAWVKDTEAEKLFRIREAEETKNSLMQVASEHIAPLQDAVDLEIATEEE
                     TLLLEAWKKYRV"
     gene            complement(517..3930)
                     /locus_tag="ACHKTW_004484"
     CDS             complement(517..3930)
                     /locus_tag="ACHKTW_004484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFO6465392.1"
                     /translation="MAVKISGVLKDGTGKPVQNCTIQLKAKRNSTTVVVNTLASENPD
                     EAGRYSMDVEYGQYSVILLVEGFPPSHAGTITVYEDSQPGTLNDFLGAMTEDDARPEA
                     LRRFEVMVEEVARNASAVAQNTAAAKKSAGDASTSAREAATHATDAADSARAASTSAG
                     QAVSSAQSASSSAGTASAKATEASKSAAAAESSKSAAATSAGAAKTSETNASASQQSA
                     ATSASTATTKASEAATSAREAAASKEAAKSSETNASSSASSSASSATAAGNSAKAAKT
                     SETNARSSETAAGQSASAAAGSKTAAASSASAASTSAGQASASATAAGKSAESAALSA
                     STATTKAGEAAEQASAAARSASAAKTSETNAKASETSAESSKTAAASSASSAASSASS
                     ASASKDEATRQASAAKGSATTASTKATEAAGSATAAAQSKNTAESAATRAETAAKRAE
                     DIASAVALEDASTTKKGIVQLSSATNSSSETLAATPKAVKSAYDNAEKRLQKDQNGAD
                     IPDKGRFLNNINAVSKTDFADKRGMRYVRVNAPAGAISGKYYPVVVMRSAGSVSELAS
                     RVIITTATRTAGDPMNNCEFNGFVMPGGWTDRGRYAYGMFWQYQNNERAIHSIMMSNK
                     GDDLRSVFYVDGAAFPVFAFIEDGLSISAPSADLVVNDTTYKFGATNPATECIAADVI
                     LDFKSGRGFYESHSLIVNDNLSCKKLFATDEIVARGGNQIRMIGGEYGALWRNDGAKT
                     YLLLTNRGDVYGGWNTLRPFAIDNATGELVIGTKLSASLNGNALTATKLQTPRLVSGV
                     EFDGSKDITLTAAHVAAFARRATDTYADADGGVPWNAESGAYNVTRSGDSYILVNFYT
                     GVGSCRTLQMKAHYRNGGLFYRSSRDGYGFEEDWAEVYTSKNLPPESYPVGAPIPWPS
                     DTVPSGYALMQGQTFDKSAYPKLAAAYPSGVIPDMRGWTIKGKPASGRAVLSQEQDGI
                     KSHTHSASASSTDLGTKTTSSFDYGTKSTNNTGAHTHSLSGSTGSAGAHTHGNGIRWP
                     GGGGSALAFYDGGGFTYVQDSQYQVSPGTSSSRSYYQRIQTQSAGAHTHSLSGTAANA
                     GAHAHTVGIGAHTHSVAIGSHGHTITVNAAGNAENTVKNIAFNYIVRLA"
     gene            complement(3995..4594)
                     /locus_tag="ACHKTW_004485"
     CDS             complement(3995..4594)
                     /locus_tag="ACHKTW_004485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFO6465393.1"
                     /translation="MRKLYAAILSAAICLAVSGAPAWASEHQSTLSAGYLHVSTNVPG
                     SDALNGINVKYRYEFTDTLGMVTSFSYAGDKNRQLTRYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDGRHSNTSLAW
                     GAGVQFNPTESVAIDIAYEGSGSGDWRTDGFIVGVGYKF"
     gene            complement(4661..8056)
                     /locus_tag="ACHKTW_004486"
     CDS             complement(4661..8056)
                     /locus_tag="ACHKTW_004486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO6465394.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPIEGPVDGLKS
                     VLLNSTPVLDTEGNTNISGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRFTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVDNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGSQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTLKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQNCDQSVPDGFGGTEPRITCNAWLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNAVEVNWIDPNNGWETATELVEDTQAIARYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISIGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGSGNPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDQSGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFLLRLTVTADDGSERLVSTARTTETTYRFRQ
                     LALGRYTLTVRAVNAWGQQGDPASVSFRIAAPAAPSRIELTPGYFQITATPHLAVYDP
                     TVQFEFWFSETRITDIRQVETTARYLGTGLYWIAASINIKPGHDYYFYIRSVNIVGKS
                     AFVESVGRASDDAEGYLDFFKGKITESHLGKELLEKVDLTEDNASRLDEFSKEWKDAN
                     DKWNAMWAVKIEQTKDGKHYVAGIGLSMEDTEEGKLSQFLVAANRIAFIDPANGNETP
                     MFVAQGNQIFMNDVFLKRLTAPTITSGGNPPAFSLTPDGRLTAKNADISGNVNANSGT
                     LNNVTINENCRVLGKLSANQIEGDLVKTVGKAFPRDSRAPERWPSGTITVRVYDDQPF
                     DRQIVIPAVAFSGAKHEREHTDIYSSCRLIVRKNGAEIYNRTALDNTLIYSGVIDMPA
                     GHGHMTLEFSVSAWLVNDWYPTASISDLLVVVMKKATAGISIS"
     gene            complement(8117..8764)
                     /locus_tag="ACHKTW_004487"
     CDS             complement(8117..8764)
                     /locus_tag="ACHKTW_004487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFO6465395.1"
                     /translation="MAATHTLPLASPGMARICLYGDLQRFGRRIDLRVKTGSEAIRAL
                     AMQIPAFRQKLSDGWYQVRIAGRDAGETELSARLNEPLANGAVIHIVPRLAGAKSGGV
                     FQVVLGAALIAVAWWNPVGWLGAAAVSGMYAAGASMILGGVAQMLAPKARTPTAASTD
                     NGKQNTYFSSLDNMVAQGNVLPVLYGEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            complement(8662..9405)
                     /locus_tag="ACHKTW_004488"
     CDS             complement(8662..9405)
                     /locus_tag="ACHKTW_004488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFO6465396.1"
                     /translation="MTETESAILAHARRCAPAESCGFVVRTPEGEKYFPCVNISGEPE
                     EYFRMSPEDWLRAEMQGEIVALVHSPPGGLPWLSETDRRLQVQSDLPWWLVCRGAIHK
                     FRCVPHLSGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHREDDWWRNGQNLYLDNLEAT
                     GLYQVPLSAAQPGDVLLCCFGSSVPNHAAIYCGDSELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHRAWHASAFTGIYNDLAAASTFE"
     gene            complement(9411..10109)
                     /locus_tag="ACHKTW_004489"
     CDS             complement(9411..10109)
                     /locus_tag="ACHKTW_004489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO6465397.1"
                     /translation="MQDIPQETHHETTRLTQSAQVVLWEIDLAEVGGERYFSCNEQNE
                     KGEPVIWQGRQYQVYPIQGTGFELNGKGSAARPTLTVSNLHGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLSTPTETDG
                     AVFPGRIMLANTCTWTYRGDECGYHGPAVADEYDQPTSDITKDKCSKCLSGCKFRNNV
                     GNFGGFLSINKLSQ"
     gene            complement(10119..>10227)
                     /locus_tag="ACHKTW_004490"
     CDS             complement(10119..>10227)
                     /locus_tag="ACHKTW_004490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO6465398.1"
                     /translation="PPYDWKPIRVVCRKWSASVGALWVTITADFEQVVA"
BASE COUNT         2144 a         3115 c         2552 g         2416 t
ORIGIN      
        1 acacccgata ctttttccag gcttccagca acaaggtttc ttcctccgtt gcgatctcca
       61 gatccacagc atcctgaagc ggcgctatat gctcactggc tacctgcatc aggctgtttt
      121 ttgtttcttc cgcctcccgg atccggaaca gtttttctgc ttccgtatcc ttcacccagg
      181 ctgtaccgtt ccacttctga aactcccctt ccggggataa ccaggtgaca ttttccggta
      241 atgagccgag ttcagaaata aataacgcgt cgccggaagc cacgtcatag accgtttttc
      301 cccgatggtc ttcaacgaga tgccacgatg cctcatcact gttgaaaaca gccacaaagc
      361 cagccggaat atctggcggt gcaatatcgg tactgtttgc aggcagacct gtatgaggag
      421 gaatatatgc gtcaccttca ccaataaatt cattagttcc ggccagcagg ttataaattt
      481 ttatggtccg tgcttgttca ctcattctga atgccattat gcgagcctca caatatagtt
      541 aaatgcgatg tttttgacgg tgttttccgc gttaccagca gcgttaacgg tgatggtgtg
      601 tccatgtgac ccaatcgcaa ccgagtgcgt atgcgcacca atacctacag tatgtgcatg
      661 tgcgccagca tttgctgcag taccagacag cgagtgggta tgagcacctg ctgactgtgt
      721 ctgaatacgt tgataatacg atctactgga agaagtcccc gggcttactt gatactgtga
      781 atcctggaca taagtgaacc caccgccatc ataaaatgct aacgcagaac cgccgcctcc
      841 tggccaacga ataccattac catgagtatg agcaccggca gaccccgtag agccactcag
      901 actgtgcgta tgcgccccgg tgttattcgt ggatttagtg ccgtaatcaa acgacgatgt
      961 ggttttcgtc cccaaatccg tactggatgc gctggcgctg tgggtatgcg atttaatgcc
     1021 atcctgttcc tgagacaata cggcacgacc actggcgggc ttgcccttaa tcgtccagcc
     1081 acgcatatca gggatcacgc ctgacggata agcggctgca agtttcgggt aggcagattt
     1141 gtcaaaagtc tgcccctgca tcagggcata accagacgga acggtatctg atggccacgg
     1201 gattggtgcg ccgactgggt agctttctgg tggaagattt ttcgaggtat aaacttctgc
     1261 ccagtcttcc tcaaaaccat agccatctct tgaagaacgg tagaacagac caccatttct
     1321 gtaatgcgcc ttcatctgca gggtccggca acttccgact ccggtataga agttaaccag
     1381 aatatagctg tcgccagagc gggtgacatt gtaagcgcct gattcggcat tccagggaac
     1441 gccaccatcc gcatcggcat atgtatccgt tgcccttctg gcaaaagcag ccacatgcgc
     1501 ggcggttaaa gtaatatctt tggaaccatc aaactcaaca ccagaaacca gtcttggcgt
     1561 ttgcagcttt gttgctgtta atgcattacc gttcagactt gcggacagtt tggttccaat
     1621 aaccagttcg ccggttgcgt tatcaatagc aaacggtctt aatgtattcc agccaccata
     1681 aacatcacct cgattggtaa gcagcaggta agttttagcg ccatcattac gccataatgc
     1741 accatactcc ccacctatca ttcgaatctg attaccacca cgcgctacaa tttcgtctgt
     1801 ggcaaaaagt tttttgcacg acaagttatc gttaacaatt aacgaatgag actcataaaa
     1861 accacgccca ctcttaaaat caaggataac gtccgccgcg atacattcag tcgccggatt
     1921 tgttgcccca aacttatagg tcgtatcatt aacaacgaga tcagcactag gtgcggatat
     1981 tgacaggcca tcttcgataa acgcaaaaac agggaaagca gcgccatcaa catagaacac
     2041 agagcgcaaa tcatcgccct tattactcat cattattgag tggatggctc gttcattgtt
     2101 ttgatattgc cagaacattc cataagcata acgccccctg tcagtccagc caccaggcat
     2161 aacaaatccg ttaaactcgc agttattcat cggatcgcct gcggttcgcg ttgccgtggt
     2221 gataatgacc cttgatgcca gttcgcttac tgagccagca gaacgcataa caacaacagg
     2281 gtaatatttt ccagatattg cacctgcagg agcgttaacc cgcacataac gcataccacg
     2341 cttatcagca aagtctgttt tactgaccgc gttaatgttg ttcaggaagc gtcccttatc
     2401 gggtatatca gcgccgttct ggtctttctg cagacgtttc tctgcattgt cataggctga
     2461 ttttactgcc tttggcgttg ccgccagcgt ttcagacgaa ctgttggtcg cactgctgag
     2521 ctgtactatc ccctttttcg tcgtacttgc atcctcaagc gccacggcgg atgcaatatc
     2581 ctctgcccgt ttagctgctg tctcggcgcg cgttgctgcg gattctgccg tattcttgct
     2641 ctgtgctgcc gccgtcgcac tgccagcagc ctctgttgcc ttcgtggatg ccgtcgtggc
     2701 gctgcctttc gctgctgatg cctgtctggt cgcctcatct tttgaagcag acgccgatga
     2761 ggccgatgac gccgccgaac tggcggacga tgcggcagcc gtttttgagg attctgcgct
     2821 ggtttccgac gctttcgcgt tcgtttcgga tgtcttcgct gcggaagctg acctcgctgc
     2881 tgcgctggcc tgttcagcgg cttcgccagc cttcgttgtg gctgttgaag cggacaatgc
     2941 agcgctttct gccgattttc cggcggcagt ggcactggct gaggcctgcc cggcacttgt
     3001 tgacgcggca ctggcagatg atgcagccgc tgtttttgag cctgccgcag ctgaggcgct
     3061 ctgtcccgct gccgtctcag aagacctggc gttcgtctcg gacgtttttg ccgccttcgc
     3121 ggaatttcct gccgccgttg ccgaggaagc ggaactactg gcgctcgagg atgcgttcgt
     3181 ttctgatgat ttcgctgcct cttttgaggc cgccgcctcc cgggctgagg tggcagcttc
     3241 tgacgctttc gtggtcgcgg tggatgcaga agtggctgct gattgttgtg acgctgaagc
     3301 attcgtttct gacgtttttg ccgcaccggc actggtggcc gccgcgcttt ttgaggactc
     3361 tgcagcggca gcactttttg atgcttcagt ggcctttgct gatgccgttc ctgcgctgga
     3421 agacgctgac tgagccgacg acacggcctg tccggctgac gtgctggctg cgcgtgctga
     3481 gtctgcagca tcagtcgcat gggttgccgc ctcacgggct gatgtgctgg catcgccggc
     3541 tgacttcttc gcggctgccg tgttctgtgc cactgcggac gcgttacgcg ccacctcttc
     3601 caccatcact tcaaaacggc gcagtgcctc cggacgggca tcatcctccg tcatggcacc
     3661 gagaaaatca ttcagcgtac cgggttgaga atcttcatac acggtgatag tcccggcatg
     3721 tgacggcggg aatccctcca ccaacagaat aacgctgtac tgaccatact caacgtccat
     3781 actgtaacgc ccggcttcat ccggattttc tgaggccagc gtgttcacca ccaccgtggt
     3841 gctgttacgt tttgctttca gctggattgt gcagttctgt accggttttc ctgtgccgtc
     3901 tttcagtaca cctgaaatct ttactgccat attcacccca caaaaaagcc cgcctgaacc
     3961 ggcgggctgt cataacactg tgttacctgg ctaatcagaa tttataaccg acacccacga
     4021 tgaaaccgtc agtgcgccag tcgccactgc cggagccttc ataagcaata tcaatggcca
     4081 cggattcggt cgggttaaac tgcacgccag ctccccacgc cagagacgtg ttgctgtggc
     4141 gaccgtcatc acttccggtc agcacatcat gcgttttccc cttgttgtca gttacgcgga
     4201 gataatcccc ggaaaaagtc gacacacggc tgtaagccac gcccgccatc gcatacgcac
     4261 tgaaccattc attcacgcgc acagagggcc ccgccattac gctgaaccag cggttacgaa
     4321 cggaatcttc atgccagcgg gtatcgctgt aacgggtaag ctggcgattc ttgtctcctg
     4381 catagctgaa agacgtcacc atccccagtg tgtccgtaaa ctcataacgg tatttcacgt
     4441 taatcccgtt cagagcatcg ctgccgggaa cgttcgtcga gacatgaaga taccccgcgc
     4501 tcagcgtgga ctgatgttca gatgcccatg caggcgcacc ggatacggcc agacaaatgg
     4561 ctgcggacaa aatggcggca taaagtttac gcataattac ctctcgcttt tctgcaaaaa
     4621 aaaggcacca tttctggtgc ccgtatatgg gttataaaat tcagctgata ctgatgcctg
     4681 cggtggcttt cttcatcacc acaaccagca aatcgctgat acttgctgtg ggataccagt
     4741 catttaccag ccatgctgac accgaaaact ccagcgtcat gtgaccgtga ccggcaggca
     4801 tatcaataac gccactgtaa atcagcgtat tatccagcgc ggtacggtta taaatttcag
     4861 caccgttttt ccgcactatc agacggcatg aggagtaaat atcagtatgc tctctctcat
     4921 gtttagcgcc gctgaatgcc accgccggaa taacaatctg ccggtcaaac ggctgatcgt
     4981 cataaaccct gacggtaatg gtccctgatg gccaccgttc cggtgcacgg gagtcccggg
     5041 ggaaagcttt gcccactgtt ttaacgagat cgccttcaat ctggttcgcg gacagttttc
     5101 ccagaacccg acagttctcg ttaatcgtga cgttgttgag cgtcccggag ttcgcattca
     5161 cgttaccgct gatatcggca tttttcgccg tcagccgccc atccggtgtc agggaaaatg
     5221 ccggtggatt tccaccactg gtaatggtgg gggccgtcag gcgtttcagg aacacgtcgt
     5281 tcatgaatat ctgattgccc tgcgccacaa acatcggcgt ttcattcccg tttgccgggt
     5341 caataaacgc gatacggtta gcggcaacca gaaactggct cagtttgcct tcctccgtgt
     5401 cctccatact gaggccaata cccgcgacat aatgtttgcc gtctttggtc tgctcaattt
     5461 tgacagccca catggcattc catttatcgt tggcatcctt ccactctttc gaaaactcat
     5521 ccagtctgct ggcgttatcc tccgtcagat cgactttttc cagcagctcc ttgccgagat
     5581 gggattcggt tatcttgcct ttgaaaaaat ccaggtaacc ttccgcatca tcgctcgccc
     5641 gaccgacgga ctccacgaat gccgatttgc caacgatgtt cacactgcgg atataaaagt
     5701 aataatcatg gcccggtttg atattgatac tggcggctat ccagtacagc cccgtgccaa
     5761 ggtagcgggc tgtggtttca acctgcctga tatcggtaat ccgcgtttcc gagaaccaga
     5821 actcaaactg taccgtcgga tcataaaccg caagatgcgg cgtggcggtg atctgaaaat
     5881 agcccggtgt cagctcaatc cgcgacggcg cagccggtgc ggcaatccgg aacgataccg
     5941 atgccggatc gccctgctgt ccccacgcat ttaccgcccg gaccgtcagc gtgtaacgcc
     6001 ccagcgccag ttgcctgaag cggtatgtgg tttccgtcgt ccgggccgtg ctgaccagcc
     6061 gctcactgcc gtcatccgct gttacggtca gacggagcag gaagctcacg cccttcacca
     6121 ccttcggcgt gtcccagcgc gccagcacct gatattcccc gctgtctgcg gtgacttcgg
     6181 cggtcaggtg ctgcaccgct ggcggcgtga caccattcac cgtgccgctc tggtcgccgt
     6241 caaagtgcgc cccgttatcc acgatggcct ctttttccgg tacatgctgc acggcagtga
     6301 tggcatacgt gccgtcatcg ttctcacgga tactcacaca gcggaacagg cgctggcgca
     6361 acgtcggcaa cttcagcccc cacacgctgt attctgcaac gccgtcagga acacggctca
     6421 ctttcacctt cacgccgtcg gtgacggact ggacctccac gctgaccgga ttgccacttc
     6481 cgtcaaccag gcttatcagc gtggtaccgg aggatggcag cgtgatttca cggtcgagcg
     6541 tcagcgtccg ggtctggctg ttcaccgcca gcacgcgccc gccgatgctg atccccgcat
     6601 agtcatcatc gcagatttca atgacatcgc ccggtacatg gcgaagccct tctgcgccca
     6661 cgctgaagtc cacggtctgc gtctccagca gctccgtttt aatcagccac agcccggcgc
     6721 ggtgtgcctg cccccgactg gtacaaccaa aggcatccat cttcgtgaca ttacgaccgt
     6781 aacgggcaat ggcctgcgta tcttcaacaa gctctgtcgc cgtctcccag ccgttgttcg
     6841 ggtcaatcca gttcacctca acggcattat ggcggtcctt cagggcgctg aagctgtagc
     6901 ggaacggcgc gccatcatcc ggcatcacca cattactgcg gttataggtc cacaccttat
     6961 ccgatggccg gtcctgcacg aacgtcagcg tctgcccgtt ccataccggc atacagcgca
     7021 tcgccgagca gaaatcactg agcacatccc acgccttgcg ctgtgtggtc agccaggcat
     7081 tacaggtgat gcgcggctcc gtgccgccaa agccgtccgg cactgactgg tcgcaattct
     7141 ggccgatgac atacagcgcc catttatcca catccgccgc accaagacgt ttccccatgc
     7201 cgtagcgcgg atgggtcagc atatcccaca gacaccaggc catgttgttg ctgtatgccg
     7261 gttttaacgt tccgtcccag ataccgctgt attgccgcgt ctgcgggtta tagttcgacg
     7321 gcacctgcag aatgcgcccg cgaagatgat aattacggct cacctgctgg ctgccgaact
     7381 gctccgagtc cacctgcacg ccgaccagtg ccgtgttcgg gtagcactgt ttcacatcga
     7441 tgatttcggt gtatgacgac cagagcgttt tgttctgcag ctggtctgtg gtgctgtccg
     7501 gcgtcatcct gcgcatccgg atattaaacg ggcgcggcgg caggttatcc accaccaccg
     7561 aggccagata ctgcgaggtg gttttgccct taatggtgat gtctttttcc gtcacccagc
     7621 caccgttacg ctgtatctga accagcaggc ggacttccga tggattcctg tccccctttg
     7681 aggtggtttc caccagtgcc tgcacaccga aggtaaagcg cagacggtcg atgtttgccg
     7741 acgtgatggt gcgggtgatc ggcgtgtcat atttcacttc cgtacccagc accgtctcgg
     7801 aaccggagga ttcaaacccc tccggcggag tctgctcctg ctcaccggcc cggaacacca
     7861 ccgtgacgcc ggagatgttg gtattcccct cagtgtccag cactggcgta ctgttcagca
     7921 gcacgctttt taatccatcc accggacctt caatcggccc ttcgctgatg gcatcgatca
     7981 cactcagcaa ctgcgtggac ttcaggttgt ccttcgcttc gcgcggagta tgccccttac
     8041 tgctgccttt acccattcct cacgctccat aaacgacaaa accgcccgga ggcggtttca
     8101 cataaaacgt ttttcatcag cgaccaatca ccacaacctg accaccatcc ccttcgtctg
     8161 ccgtgctgat ctcctgagag accacccgcg accccacgcg catttcaccg tacagaacgg
     8221 gcagaacatt gccctgggca accatgttat ccagtgagga gaaataggtg ttctgtttgc
     8281 cgttatctgt acttgctgcc gtgggcgtcc tggctttcgg tgccagcatc tgcgccactc
     8341 cgcccaggat catactggcc cctgccgcat acatgcccga tacagccgcg gcacccagcc
     8401 agcccacagg gttccaccat gccaccgcaa tcagcgccgc ccccagcacc acctgaaaca
     8461 caccgccact tttagctccc gccagacgcg gcacgatgtg gatcacggca ccatttgcca
     8521 gcggctcatt aagacgggca gacaattcgg tttcacctgc atcacgcccg gcaatgcgta
     8581 cctggtacca gccgtcgctc agtttctgac gaaacgccgg gatctgcatg gccagcgccc
     8641 ggatggcttc ggaccccgtt ttcactcgaa ggtcgatgcg gcggccaaat cgttgtaaat
     8701 ccccgtaaag gcagatgcgt gccatgcccg gtgacgccag agggagtgtg tgcgtcgctg
     8761 ccatttgtcg gtgtacctct ctcgtttgct cagttgttca ggaatatggt gcagcagctc
     8821 gctgtcacca cagtaaatgg cggcatgatt cggcaccgat gaaccaaaac agcacagcag
     8881 cacatcgccc ggctgcgccg ctgacaacgg cacctgatac agccctgtgg cctccagatt
     8941 atccagatag agattctgac cgttacgcca ccagtcatcc tcgcgatgaa aatccggcat
     9001 ctcaatcccc gccagatggt aagcatcccg gaacagcgtg taacagtccg tcaccccgtg
     9061 ctcaaagcgc cgcccggaaa gatgtggtac acagcggaac ttgtgaatcg ccccccggca
     9121 aaccagccac cacggcaaat cactctgcac ctgcagccgc cggtcggtct cactcagcca
     9181 gggcagacca ccgggggggc tgtggaccag cgccacaatc tcaccctgca tctctgcccg
     9241 cagccagtcc tccggcgaca tccggaaata ctcctccggc tcaccggaga tattcacgca
     9301 gggaaaatat ttttccccct ccggtgttct caccacgaag ccgcacgact ccgctggcgc
     9361 acatcgccgg gcgtgcgcca gaatcgctga ttctgtctct gtcatgggat tcactgcgaa
     9421 agtttgttaa tggaaaggaa gccgccaaag ttgccgacgt tattgcgaaa cttacagccg
     9481 ctcaggcatt tgctgcattt atccttcgtg atatcggacg tcggctgatc atattcatcc
     9541 gcgaccgccg gaccgtgata accgcactca tcaccgcgat aggtccaggt gcaggtgttg
     9601 gccagcatga tacgtcccgg aaaaacggca ccgtccgttt ccgtcggcgt ggacagtaca
     9661 aaagaggcac tcaccgcgct cagttcgctg cactgctcga tgcgccagcg gctgatcacc
     9721 tcctgctccg gatcggcgtc actgtttccg ttgacgaagt tcaccgcatc cagaaaacgg
     9781 gcgtaaacct tacgccggac caccgttcct ccgaccagac tctgcaggtc ttccgccatc
     9841 ccggtgacca tgccgtgcag gttagaaacc gtcagtgtcg gacgggcagc actgcccttg
     9901 ccgttcagtt caaatcccgt cccctgaatg gggtatacct gatactgccg cccctgccag
     9961 ataaccggct cacctttttc gttctgctca ttacaggaaa aataacgttc accaccgacc
    10021 tctgccagat cgatttccca tagcaccacc tgggctgact gagtgaggcg tgtcgtctca
    10081 tgatgtgttt cctgtggaat atcctgcatc agagcctcct atgccacgac ctgttcaaaa
    10141 tctgccgtta tggttaccca cagcgccccc acgcttgccg accatttacg acaaaccacc
    10201 ctgatcggtt tccagtcata aggtggc
//
LOCUS       DAZFQD010000167        10209 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.167, whole genome shotgun sequence.
ACCESSION   DAZFQD010000167 DAZFQD010000000
VERSION     DAZFQD010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10209
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.167"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1231
                     /locus_tag="ACHKTW_004491"
     CDS             <1..1231
                     /locus_tag="ACHKTW_004491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HFO6465399.1"
                     /translation="KLIPQMNYLMVVVALFFLNAVIFLFMLMKYFTNKQILPTLILSL
                     AFLSGLIYLVETIVIIHKPINGSTLIQTKSNDVSIFYIFRQLSFICLTSLALFCYGKD
                     NILDNNKKKTGILLLALIPFLVFPLLAHNLSSYNADYSLYVVDYCPDNHTATWGINYT
                     KILVCLWAFLLFFIIMRTRLASELWPLIALLCLASLCCNLLLLTLDEYNYTIWYISRG
                     IEVSSKLFVVSFLIYNIFQELQLSSKLAVHDVLTNIYNRRYFFNSVESLLSRPVVKDF
                     CVMLVDINQFKRINAQWGHRVGDKVLVSIVDIIQQSIRPDDILARLEGEVFGLLFTEL
                     NSAQAKIIAERMRKNVELLTGFSNRYDVPEQMTISIGTVFSTGDTRNISLVITEADKA
                     LREAKSEGGNKVIIHHI"
     gene            1412..2902
                     /gene="dgcJ"
                     /locus_tag="ACHKTW_004492"
     CDS             1412..2902
                     /gene="dgcJ"
                     /locus_tag="ACHKTW_004492"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0043709 - cell adhesion involved in
                     single-species biofilm formation [Evidence IEA];
                     GO_process: GO:1902201 - negative regulation of
                     bacterial-type flagellum-dependent cell motility [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcJ"
                     /protein_id="HFO6465400.1"
                     /translation="MKLHHRMLRHFIAASVIVLTSSFLIFELVASDRAMSAYLRYIVQ
                     KADSSFLYDKYQNQSIAAHVMRALAAEQSEVSPEQRRAICEAFESANNTHGLNLTAHE
                     YPGLRGTLQTASTDCDTIVEAAALLPAFDQAVEGNRHQDDYGSGLGMAEEKFHYYLDL
                     NDRYVYFYEPVNVEYFAMNNWSFLQSGSIGIDRKDIEKVFTGRTVLSSIYQDQRTKQN
                     VMSLLTPVYVAGQLKGIVLLDINKNNLRNIFYTHDRPLLWRFLNVTLTDTDSGRDIII
                     NQSEDNLFQYVSYVHDLPGGIRVSLSIDILYFITSSWKSVLFWILTALILLNMVRMHF
                     RLYQNVSRENISDAMTGLYNRKILTPELEQRLQKLVQSGSSVMFIAIDMDKLKLINDT
                     LGHQEGDLAITLLAQAIKQSIRKSDYAIRLGGDEFCIILVDSTPQIAAQLPERIEKRL
                     QHIAPQKEIGFSSGIYAMKENDTLHDAYKASDERLYVNKQNKNSRS"
     gene            2945..3448
                     /gene="yeaK"
                     /locus_tag="ACHKTW_004493"
     CDS             2945..3448
                     /gene="yeaK"
                     /locus_tag="ACHKTW_004493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HFO6465401.1"
                     /translation="MTEMAKGSVTHQRLIALLSQEGANFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHSKLKLVADPLLFERFDEIAFNAGMLDKSVILKTTDYLRIAQPEL
                     LNFRRTA"
     gene            complement(3449..3553)
                     /gene="yoaI"
                     /locus_tag="ACHKTW_004494"
     CDS             complement(3449..3553)
                     /gene="yoaI"
                     /locus_tag="ACHKTW_004494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YoaI"
                     /protein_id="HFO6465402.1"
                     /translation="MNDQMFVETLIITSSFFAIAVVLVLSVLLIERTG"
     gene            3723..4169
                     /gene="yeaL"
                     /locus_tag="ACHKTW_004495"
     CDS             3723..4169
                     /gene="yeaL"
                     /locus_tag="ACHKTW_004495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFO6465403.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            complement(4126..4947)
                     /gene="nimR"
                     /locus_tag="ACHKTW_004496"
     CDS             complement(4126..4947)
                     /gene="nimR"
                     /locus_tag="ACHKTW_004496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NimR"
                     /protein_id="HFO6465404.1"
                     /translation="MMHRLNLNGYEPDRHHEAAVAFCIHAGTDELTSPVHQHRKGQLI
                     LALHGAITCTVENALWMVPPQYAVWIPGGVEHSNQVTANAELCFLFIEPSAVTMSTTC
                     CTLKISPLCRELILTLANRTTTQRAEPMTRRLIQVLFDELPQQPQQQLHLPVSSHPKI
                     RTMVEMMAKGPVEWGSLGQWAGFFAMSERNLARLIVKETGLSFRQWRQQLQLIMALQG
                     LVKGDTVQKVAHTLGYDSTTAFITMFKKGLGQTPGRYIAGLTTVSPQSAKQDPRQ"
     gene            5044..6225
                     /gene="nimT"
                     /locus_tag="ACHKTW_004497"
     CDS             5044..6225
                     /gene="nimT"
                     /locus_tag="ACHKTW_004497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
                     /protein_id="HFO6465405.1"
                     /translation="MTCSTSLSGKNRIVLIAGILMIATTLRVTFTGAAPLLDTIRSAY
                     SLTTAQTGLLTTLPLLAFALISPLAAPVARRFGMERSLFAALLLICAGIAIRSLPSPY
                     LLFGGTAVIGGGIALGNVLLPGLIKRDFPHSVARLTGAYSLTMGAAAALGSAMVVPLA
                     LNGFGWQGALFMLMCFPLLALFLWLPQWRSQQHANLSTSRALHTRGIWRSPLAWQVTL
                     FLGINSLVYYVIIGWLPAILISRGYSEAQAGSLHGLLQLATAAPGLLIPLFLHHVKDQ
                     RGIAAFVALMCAVGAVGLCFMPAHAITWTLLFGFGSGATMILGLTFIGLRASSAHQAA
                     ALSGMAQSVGYLLAACGPPLMGKIHDANGDWSVPLMGVAILSLLMAIFGLCAGRDKEI
                     R"
     gene            6280..6627
                     /gene="yeaO"
                     /locus_tag="ACHKTW_004498"
     CDS             6280..6627
                     /gene="yeaO"
                     /locus_tag="ACHKTW_004498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HFO6465406.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFATFREQYLAELAQHEQEGKRLADIAKKQPLTLLYAAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(6649..6903)
                     /gene="yoaF"
                     /locus_tag="ACHKTW_004499"
     CDS             complement(6649..6903)
                     /gene="yoaF"
                     /locus_tag="ACHKTW_004499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HFO6465407.1"
                     /translation="MKIISFALPCLLVLAGCSTSSQPEAPKPPQIGMANPASVYCQQK
                     GGTLIPVQTAQGVSNNCKLPGGETIDEWALWRRDHPAGEK"
     gene            7086..8111
                     /gene="dgcP"
                     /locus_tag="ACHKTW_004500"
     CDS             7086..8111
                     /gene="dgcP"
                     /locus_tag="ACHKTW_004500"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HFO6465408.1"
                     /translation="MSDQIIARVSQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKV
                     DVEARLQHIMFARNSQKMHIPENFTVSWDYSLCKRAIDENCFFSDEVPDRWGDCIAAR
                     NLGITTFLSTPIHLPDGSFYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALV
                     EQLREANAALIAQSYTDSLTGLPNRRAIFENLTTLFSLARHLNHKIMIAFIDLDNFKL
                     INDRFGHNSGDLFLIQVGERLNTLQQNGEVIGRLGGDEFLVVSLNNENADISSLRERI
                     QQQIRGEYHLGDVDLYYPGASLGIVEVDPETTDADSALHAADIAMYQEKKHKQKTPFV
                     AHPALHS"
     gene            8144..8242
                     /gene="yoaK"
                     /locus_tag="ACHKTW_004501"
     CDS             8144..8242
                     /gene="yoaK"
                     /locus_tag="ACHKTW_004501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HFO6465409.1"
                     /translation="MRIGIIFPVVIFITAVVFLAWFFIGGYAAPGA"
     gene            8245..8319
                     /gene="yoaJ"
                     /locus_tag="ACHKTW_004502"
     CDS             8245..8319
                     /gene="yoaJ"
                     /locus_tag="ACHKTW_004502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HFO6465410.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
     gene            complement(8378..8626)
                     /gene="yeaQ"
                     /locus_tag="ACHKTW_004503"
     CDS             complement(8378..8626)
                     /gene="yeaQ"
                     /locus_tag="ACHKTW_004503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFO6465411.1"
                     /translation="MGILSWIIFGLIAGILAKWIMPGKDGGGFFMTILLGIVGAVVGG
                     WISTLFGFGKVDGFNFGSFVVAVIGAIVVLFIYRKIKS"
     gene            complement(8853..10061)
                     /gene="insQ"
                     /locus_tag="ACHKTW_004504"
     CDS             complement(8853..10061)
                     /gene="insQ"
                     /locus_tag="ACHKTW_004504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFO6465412.1"
                     /translation="MKRLQAFKFQLRPGGQQECEMRRFAGACRFVFNRALARQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDSPSQPLQQSLKDLERAYKNFFRKRAAFPR
                     FKKRGQNDAFRYPQGVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGKWY
                     ISIQTESEVSTPVHPSASMIGLDAGVAKLATLSDGTVFGPVNSFQKNQKTLARLQRQL
                     SRKVKFSNNWQKQKRKIQRLHSRIANIRRDYLHKVTTTVSKNHAMIVIEDLKVSNMSK
                     SAAGTVSQPGRNVRAKSGLNRSILDQGWYEMRRQLEYKQLWSGGQVLAVPPAYTSQRC
                     ACCGHTAKENRLSQSQFRCQVCGYTANADVNGARNILAAGHAVLACGEMVQSGRSLKQ
                     EPTEMIQATA"
     gene            10069..>10209
                     /locus_tag="ACHKTW_004505"
     CDS             10069..>10209
                     /locus_tag="ACHKTW_004505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO6465413.1"
                     /translation="MILWSVKKETDIRRGRHCVFLMHVHLVFVTRYRLLIFDHDATEK
                     LRT"
BASE COUNT         2494 a         2479 c         2392 g         2844 t
ORIGIN      
        1 aaaactcatc ccacagatga attacctgat ggttgttgtg gctttgtttt tccttaacgc
       61 cgtcattttt cttttcatgt taatgaaata tttcactaac aaacaaattt taccaacact
      121 cattttaagc cttgcatttt taagtggcct tatctattta gttgaaacca ttgtaattat
      181 ccataaacca attaacggca gtacactgat ccagacaaag tcgaatgatg tttctatttt
      241 ctatattttc cgccaactca gttttatttg tttaacctct ctggcactct tttgttatgg
      301 aaaagacaac atccttgaca acaataagaa aaaaacggga atcctgttgc tggcgctgat
      361 tcctttttta gtttttcccc ttctggcaca caatctgagc agttataacg ctgactattc
      421 tttgtatgtc gtcgactact gtccggacaa ccatactgcg acctggggaa tcaactatac
      481 aaaaatattg gtttgtctgt gggcattttt actgttcttt attatcatgc gtacacgatt
      541 agccagcgaa ctatggccat taatagcatt attatgtctg gcatcgctat gctgcaactt
      601 acttctactg actctggatg agtataatta caccatctgg tatatcagtc gcgggattga
      661 agtttccagt aaactgtttg ttgtgtcttt tctgatttat aacatttttc aggagctgca
      721 actctccagc aaactggcag ttcatgatgt gctgaccaat atttataatc ggcgctactt
      781 tttcaacagc gtagagtcat tattgtcgcg acctgttgtt aaggacttct gtgtcatgct
      841 ggttgatatt aatcagttca aacgcatcaa tgcccaatgg ggacatcgtg tgggtgataa
      901 agtgctggtt tcaattgtcg atattatcca gcaaagcatc cgccccgatg atattttagc
      961 gcgtctggag ggtgaggtgt ttggcttgct atttaccgaa ctcaatagtg cccaggcaaa
     1021 aatcattgcg gaacgtatgc gtaaaaatgt cgaactcctg accggcttta gtaacagata
     1081 tgatgttcct gaacaaatga ccatcagtat tggcacggtt ttttcaacgg gtgacacgcg
     1141 taatatctcg cttgtcatca cggaagcaga taaagcctta cgcgaagcga aaagcgaggg
     1201 gggcaacaaa gtgattattc atcatattta agtgcaaaaa tattcagagc catgcttttt
     1261 gcgtggcttt tgcatacaat ttattactat tacccctaaa tttcccctcc ccagcggcgc
     1321 agagatgagt ataattagcg cccttgtgcc aggccgcaat cgaactttat ctggttttct
     1381 cgtttcacta accgaaggag tgccatttat catgaaattg caccatagaa tgctccggca
     1441 ttttatcgcc gcaagtgtca ttgtgctgac atcttccttc cttatttttg aacttgtcgc
     1501 cagcgacaga gcaatgagtg cctatctgcg ctatatcgtg cagaaagcag actcctcctt
     1561 tctttatgat aagtatcaga atcagagtat tgccgcgcat gtgatgcgcg ctctcgctgc
     1621 tgagcagtcg gaagtgtcgc cagaacagcg gcgcgccatc tgcgaggctt ttgagtctgc
     1681 caataatacc catggcttaa acctgactgc ccatgaatac ccgggcttac gcggcacact
     1741 acaaaccgca tccactgact gcgacacaat tgtggaagct gcagcactat tacccgcttt
     1801 tgatcaggca gtggaaggca accgccacca ggatgattac ggttcaggtc ttgggatggc
     1861 cgaagagaaa tttcactatt atctcgatct caatgaccgc tatgtctatt tttatgagcc
     1921 ggttaatgtt gaatactttg cgatgaataa ctggtccttc ctgcagtcag gaagtattgg
     1981 catcgatcgc aaagatattg aaaaggtatt taccgggcgt accgtattgt cgagcattta
     2041 ccaggatcag cgtactaaac agaacgtgat gagtttgctg acgccggtat atgtcgcagg
     2101 gcagctaaaa gggattgtgc tgctggatat taacaaaaac aatttgcgga atatctttta
     2161 cactcatgac cgccctctcc tctggcgttt tctcaatgtc acgctaaccg ataccgattc
     2221 ggggcgcgac attatcatca accagagcga agataatctg ttccagtatg tcagttacgt
     2281 ccatgactta ccgggcggca ttcgtgtctc gttatccatt gatattcttt actttatcac
     2341 gtcttcgtgg aaaagcgttc tgttctggat tttgacggcg ttaattttgc tgaatatggt
     2401 gcggatgcac ttccgcttat accaaaatgt gtcgcgagaa aatattagtg atgcgatgac
     2461 tggactgtat aatcgcaaaa ttttaacccc tgaactggag cagcggttgc agaaactggt
     2521 gcaatccggt tcttcggtga tgtttattgc aattgacatg gacaagttaa agctaataaa
     2581 tgacaccctc ggtcatcagg aaggggattt agcgattacg ttgttagctc aggcgattaa
     2641 acaatcgatt cgtaaaagtg attatgccat ccgactcggt ggcgatgaat tctgcatcat
     2701 tcttgtcgat tcgacgccgc aaattgcagc acaactgcct gaacgtatcg aaaaacgtct
     2761 gcaacatatc gcgccgcaga aagagatcgg cttctcttcc ggtatttacg cgatgaaaga
     2821 aaacgatacg ttacatgatg cgtataaagc ttccgatgag cgtttatatg tcaataagca
     2881 gaacaaaaac agccgttcat gataaccttc tgtggttgtt tgcttgtaat ctcaggagcg
     2941 tgaaatgact gaaatggcta aaggaagcgt gacgcatcag cggttaatcg ctttattatc
     3001 acaagaaggt gctaacttcc gcgtcgttac ccatgaagcg gtaggtaaat gtgaagcggt
     3061 gtcagaaatt cgtggcaccg ctttaggcca gggagctaag gcactggtat gtaaagtcaa
     3121 aggcaatggc gtaaatcaac atgttctggc gatcctcgct gccgatcaac aggccgatct
     3181 gagccaactc gccagccata taggcggatt acgcgcttcg ttggccagcc cggcggaagt
     3241 cgatgaactc acaggctgtg tcttcggcgc gatcccccct ttcagcttcc attcaaaact
     3301 caaactggtt gccgaccctc tactcttcga acgatttgat gaaatcgcct tcaatgcagg
     3361 gatgctggat aaatccgtta ttttgaaaac cacggattat ttgcgcattg cgcaaccaga
     3421 actgctcaat ttccgccgca ctgcgtaact agcctgtccg ttcaataagc agaacggaca
     3481 aaaccagcac aacagcaatg gcaaaaaacg atgacgtgat aatcagtgtc tcgacgaaca
     3541 tttgatcgtt catagcatgc ccccgggaga tagctgtttt cacgttattg ttagcgtgca
     3601 caaatggcag tttgatgaca gttcgcctga ttttttatgc aaaaaacgta aagattttct
     3661 acttccttcc tgcagcaagc gtaaagtaag caggcttatt attttttggc aaggaaacca
     3721 cgatgtttga tgtcactttg ctgatcctgc tcgggttagc tgcgctgggc tttatcagtc
     3781 ataacaccac tgtcgccgtt tcaattctgg tgttgatcat tgtccgcgtc acaccgttaa
     3841 gcaccttttt cccgtggatt gaaaaacagg ggctaagtat cgggataatc attctgacga
     3901 ttggcgttat ggcccccatc gccagcggga cgctaccgcc ctcaaccttg attcactcgt
     3961 ttctgaactg gaaatcgctg gtggcaattg cagtaggcgt tattgtctcc tggctgggtg
     4021 ggcgcggcgt gaccttaatg ggcagccagc cgcaactggt cgccgggctg ctggtaggca
     4081 ctgttttagg cgtggcgctg tttcgcggcg taccggtcgg cccgcttatt gccgcgggtc
     4141 ttgtttcgct gattgtgggg aaacagtagt taatcccgca atatatcggc ctggcgtttg
     4201 ccccaggcct ttcttaaaca tagtaataaa ggccgttgtt gaatcatacc ccagcgtatg
     4261 agcaactttc tgtacagtat cgcctttcac taatccctga agtgccataa tcagctgtaa
     4321 ctgctgacgc cactggcgaa aactcaaccc cgtctctttt actatcagtc gcgcaagatt
     4381 gcgttcactc atcgcaaaaa agccagccca ttgccctaat gacccccact cgacgggccc
     4441 tttcgccatc atctccacca tcgtgcggat tttaggatga gaagaaacgg gcaggtgcag
     4501 ctgttgttgt ggctgctgag ggagttcatc aaacaggacc tgaattaatc tgcgggtcat
     4561 cggctcagca cgctgagtcg tcgtcctgtt tgccagggtt aaaatcaatt cccggcataa
     4621 gggcgatatt ttcaatgtgc aacatgttgt cgacattgtc actgcgctgg gttcgataaa
     4681 cagaaaacag agttcagcgt tagctgtcac ttgattgcta tgttccaccc cgcccggtat
     4741 ccaaaccgca tattgcggcg gcaccatcca caaagcattt tccaccgtac aggtaattgc
     4801 accatgtaat gctagtatca gctgtccttt gcggtgctga tgtacaggac tggttaactc
     4861 atccgttccg gcgtgaatac aaaatgcgac cgcggcttca tggtgtcggt cgggttcata
     4921 gccattgaga ttcaacctgt gcatcatttt gtccgaattt agcgataatt tgtcatttta
     4981 gcttgattca acataacaat aaaaacggta aggtacagcc tcgtttgtaa caatgagaag
     5041 catatgacct gttcaacttc attaagcggc aaaaacagga ttgtccttat cgctggcatt
     5101 ctgatgattg ccacaacatt acgcgtcacc tttaccggcg cagcgccgtt actggatacg
     5161 attcgttccg cttactcgct gacgacagcg caaaccggct tattaaccac cctgccatta
     5221 ttggcctttg cgctaatctc acctttggct gccccggtgg cgcgacgttt tggtatggaa
     5281 cgtagcctgt ttgccgcgtt acttttgatc tgtgcaggta tcgcaattcg ttctctccct
     5341 tcgccttact tattatttgg cggtacagcg gtcattggcg gtgggattgc attaggcaat
     5401 gtcttactgc caggattaat taaacgcgat ttccctcatt ccgtcgccag acttaccggc
     5461 gcatattccc tgacaatggg agctgcagcg gcactgggat cggctatggt cgtgccgctg
     5521 gctttgaacg gttttggctg gcaaggcgcg ttgttcatgc tgatgtgttt tcctctgctg
     5581 gctctttttt tatggctgcc acagtggcga agccaacaac atgcaaattt gagtacctcg
     5641 cgcgccttac atactcgggg tatctggcgt tcgccgcttg cctggcaggt cacattgttt
     5701 cttgggatca actcactggt ctattacgtg attattggct ggcttccggc gatcctcatc
     5761 agtcgcggct atagcgaagc acaggcgggt tcactgcatg gtttgctgca actagccaca
     5821 gcagctcccg gtttgctgat ccctcttttc ttacatcatg tgaaagatca gcgtggtatt
     5881 gcagcgttcg ttgccttgat gtgcgcagtg ggcgctgttg ggctctgctt tatgccagcg
     5941 cacgcgatca cctggactct gcttttcggt tttggttccg gcgcaacaat gatactgggg
     6001 ttgacgttca ttggtcttcg ggcaagttct gcgcatcagg cggcggcact ctcggggatg
     6061 gcacaatccg tcgggtattt gctggcagcc tgtgggccgc cgctgatggg taaaatacac
     6121 gatgctaacg gtgactggtc cgtaccactg atgggtgttg ccatactttc actactgatg
     6181 gcgattttcg gactttgcgc cgggagagac aaagaaattc gctaatatcc ggtgctatag
     6241 tgacgtgaca aatcatgcgt gaaagggaga acaaacacga tgaatattca gtgcaaacgc
     6301 gtttatgatc cggctgaaca gagcgatggt tatcgcgtac tggtcgaccg cctctggccg
     6361 cgcggtatca aaaaaaccga tttagccctt gatgagtggg ataaagaaat cacgccgtca
     6421 acggaactgc gcaaagcctt tcacggcgaa gtcgtcgatt ttgcaacctt tcgcgagcaa
     6481 tatcttgcag aactggcgca acacgagcaa gaaggaaagc ggctggcgga catcgccaaa
     6541 aaacagccgc tgaccctgct ctacgcagca aaaaacacca cgcagaacca tgcgctggtg
     6601 ctggccgact ggctacgtag cttgtgattt tagtacagca tccggcggtt atttttcacc
     6661 agccggatgg tcccgtcgcc acaatgccca ttcatcaatc gtttcaccgc ccggtaattt
     6721 gcaattgttg ctgacccctt gcgctgtctg cactggaatg agcgtcccgc ccttctgctg
     6781 gcaatagacc gacgccggat ttgccatacc aatctgcggc ggtttaggtg cttctggctg
     6841 agaagaggtt gaacaaccag ccaggaccag caagcaaggc aaagcaaaac tgataatttt
     6901 catttattga tctcacacat ttatctacgc ttagagtatc gcggtatcgt tttgttttgc
     6961 agcactattt ttattacatt cactcaaaac atattacgtc ttgtttcatc tttgttgatg
     7021 atgttttatc atgcctgcaa agattaaata atcagcattt acccgccgta tcctggagtt
     7081 gtttcgtgtc agatcagatt atcgcccgcg tctcgcaatc ccttgccaaa gaacagtcac
     7141 tggaaagtct ggtccgacag cttctggaga tgctggaaat ggtcactgat atggaatcaa
     7201 cctacctgac caaagtggat gtcgaagcgc gcctgcagca tattatgttt gcccgtaaca
     7261 gccagaaaat gcacatcccg gagaatttta ccgtctcgtg ggattactcg ttatgcaaac
     7321 gcgctattga tgaaaactgc tttttcagcg atgaagtccc tgaccgttgg ggcgactgta
     7381 ttgcggcacg caatcttgga atcaccacat ttctgagtac accaattcac ttaccggatg
     7441 gatcattcta tggcacgctt tgcgccgcca gcagtgagaa gcgccagtgg agtgaacgcg
     7501 cggaacaggt tttacagtta ttcgccggac tgattgcaca atatattcaa aaagaggcgt
     7561 tggttgaaca gctgcgcgaa gccaatgccg cgctgattgc gcaatcgtat accgactcgt
     7621 taaccgggct accgaatcgg cgggcgattt ttgaaaatct gacgacgctg ttttctctcg
     7681 cccggcatct taaccataag ataatgatcg cgtttatcga tctggataac ttcaaattaa
     7741 tcaatgaccg ttttggtcat aatagtggcg atctgtttct cattcaggtt ggcgagcgcc
     7801 ttaatacgct ccagcaaaat ggcgaagtta ttggtcgtct cggcggtgat gagtttttgg
     7861 ttgtttcact aaacaacgag aatgcggata tttcgtcgct gcgagaacgt attcaacagc
     7921 aaatacgtgg agaatatcac ttaggtgatg ttgatttgta ttatcccggt gccagtcttg
     7981 gcatagtaga agtcgatcct gaaacaaccg atgcagacag tgccctgcat gctgccgata
     8041 ttgcgatgta tcaggagaaa aaacacaaac agaaaacacc ttttgtcgcg catccagcgc
     8101 tacattcctg aggcgtattc acatcctttt gattggtgat aacatgcgaa tcggtattat
     8161 ttttccggtt gtaatcttta ttacagcggt cgtattttta gcatggtttt ttattggcgg
     8221 ctatgctgcc ccgggagcat aaagatgaaa aaaacaacga ttattatgat gggtgtggcg
     8281 attattgtcg ttctcggcac tgagctggga tggtggtaac gtcacctcta aaaaatagca
     8341 aaggctgcct gtgtgcagcc tttgtgcaat ttaagcgtta acttttaatc ttcctgtaga
     8401 taaatagcac gacaatcgca ccaataacgg caaccacgaa gctgccaaaa ttgaagccat
     8461 cgactttacc aaagccaaac agtgtgctga tccatccgcc gactacggca ccgactatcc
     8521 ccagcagaat agtcataaag aatccaccac catctttacc tggcatgatc cacttcgcca
     8581 gaataccggc aataagccca aaaataatcc atgacagaat gcccattgtt tcctcactta
     8641 tctgttttgc attagcgggt tagtcgctga taaaaagcat agcacaacat cgggagggca
     8701 agatttgtga cgagcatcac ggaggatttt tttgcgatgg cgcagaaatt gcgccatcaa
     8761 cgatcggtga taattaccaa ccacaaacat catgttcgtt ttccgtgtct tgacatcctc
     8821 cacgccctga atgacgagga tccctgctac gttcaggctg tcgcctgaat catttcggtg
     8881 ggttcctgct tcaacgagcg gcctgactgc accatctctc cacaggcaag aacggcgtgc
     8941 cccgccgcta aaatgttacg agcgccgttt acatcggcgt tcgctgtata tccacatacc
     9001 tggcatctga attgactttg tgacaggcga ttttctttcg ctgtatgacc acagcacgcg
     9061 caacgctggc ttgtgtacgc tggcggaaca gcaagcacct gaccgccact ccagagctgc
     9121 ttgtactcaa gctggcggcg catttcatac cagccctgat ccagtatcga acggtttaaa
     9181 cctgattttg cccggacatt gcgccccggc tggctgaccg tacccgctgc tgactttgac
     9241 atgttgctga ccttcaaatc ctcaatgact atcattgcgt ggtttttgct gacggtcgtt
     9301 gtgactttat gaaggtagtc cctgcggata tttgcgatac gggaatgcag tcgctgtatt
     9361 ttgcgtttct gcttctgcca gttattgctg aatttgacct tgcggcttaa ctgtcgctga
     9421 agtctcgcca gcgtcttctg gtttttctga aaactgttta caggcccaaa gactgtgcca
     9481 tctgacagcg tggcgagttt agccacgcca gcatccagcc cgatcattga tgctgaaggg
     9541 tgaaccggag ttgatacttc actttctgtc tgaatactga tgtaccactt accgcaggac
     9601 tggctgaccg tgacattttt cacaacaccc gtgacctgac ggctattccg gtagcgcatc
     9661 cagcccagtt tcggcagaaa aatacggctg ttttcctgat cgagctttac gccctgcggg
     9721 tagcggaatg catcattctg tccccgcttt ttgaatcggg gaaaagcagc ccgcttccgg
     9781 aagaagtttt tgtaagcccg ctcaaggtct ttcagtgact gttgcaatgg ctgtgagggc
     9841 gaatctttaa gccattgcgt ttcagtggcg tttttccact caaccagcca ggaagccatt
     9901 ttcccgtaag ggatgtattt attaccggcc tcatgattct cattctgacg tgccagagca
     9961 cgattgaaaa cgaaacgaca tgcgcctgcg aagcgcctca tttcacactc ctgttgacca
    10021 cctggtctta actggaattt aaatgcctgt aatcgtttca caggtcaaat gatactttgg
    10081 tctgtgaaaa aagaaaccga tatccggcgt ggaaggcatt gtgttttcct gatgcatgtt
    10141 cacctggtct ttgtcaccag ataccgactc ctgatttttg accatgacgc gacagaaaaa
    10201 ctacgcact
//
LOCUS       DAZFQD010000168        10209 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.168, whole genome shotgun sequence.
ACCESSION   DAZFQD010000168 DAZFQD010000000
VERSION     DAZFQD010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10209
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.168"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1330
                     /gene="dgcT"
                     /locus_tag="ACHKTW_004506"
     CDS             <1..1330
                     /gene="dgcT"
                     /locus_tag="ACHKTW_004506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HFO6465414.1"
                     /translation="TVLVSLLFGLALVLVNSWFNQPGVEEVVPRSTYLMVMIALFFID
                     TVAFIFMQLYFIYDRRQFSNCILSLAFLSCLIYFVKTVIIIQQIIEGRLTSSVVQNDI
                     AIYYLFRQMSLCILIFLALVNKVSENTKQRNLFSKKMTLCISLFFVFGGPIVAHILSS
                     HYESYNLHIAELTNENGQVVWKASYVTIMIFMWLTLLSVNLYFNGLRYDIWNGVTVIA
                     FCAVLYNISLLFMSRYSVSTWYISRTIEVVSKLTVMVIFMCHIFSALRVTKNIAHRDP
                     LTNIFNRNYFFNELTVQSASAKKTPYCVMIMDIDHFKKVNDTWGHPVGDQVIKTVVNI
                     IGKSIRPDDLLARVGGEEFGVLLTDIDTERAKALAERIRENVERLTGDNPEYAIPQKV
                     TISIGAVVTQENALNPNEIYRLADNALYEAKETGRNKVVVRDVVNFCESP"
     gene            complement(1371..2533)
                     /locus_tag="ACHKTW_004507"
     CDS             complement(join(1371..2272,2272..2533))
                     /locus_tag="ACHKTW_004507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS3 family transposase"
                     /protein_id="HFO6465415.1"
                     /translation="MTKTVSTSKKPRKQHSPEFRSEALKLAERIGVTAAARELSLYES
                     QRYNWRSKQQNQQTSSERELEMSTEIARLKRQLAERDEELAILPKGRDILREAPEMKY
                     VFIEKHQAEFSIKAMCRVLRVARSGWYTWCQRRTRISTRQQFRQHCDSVVLAAFTRSK
                     QRYGAPRLTDELRAQGYPFNVKTVAASLRRQGLRAKASRKFSPVSYRAHGLPVSENLL
                     EQDFYASGPNQKWAGDITYLRTDEGWLYLAVVIDLWSRAVIGWSMSPRMTAQLACDAL
                     QMALWRRKRPRNVIVHTDRGGQYCSADYQAQLKRHNLRGSMSAKGCCYDNACVESFFH
                     SLKVECIHGEHFISREIMRATVFNYIECDYNRWRRHSWCGGLSPEQFENKNLA"
     gene            2600..3127
                     /locus_tag="ACHKTW_004508"
                     /pseudo
     CDS             2600..3127
                     /locus_tag="ACHKTW_004508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309324.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
     gene            3124..4110
                     /gene="ycdU"
                     /locus_tag="ACHKTW_004509"
     CDS             3124..4110
                     /gene="ycdU"
                     /locus_tag="ACHKTW_004509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcdU"
                     /protein_id="HFO6465416.1"
                     /translation="MIPDYLTFIRFQDKRNLIYIYAIGLILIGFYWKNAGFTFPSEDI
                     GVVSGILALVLYNFIFDLKAYWAYKCVTKNIDFSWFKKKQNHKIELFLTQPLVAGFLS
                     LIMLSAMSWGLYQLLPSLYALFLISLLGPLVIFLLFRMIRTSYVKQVAISVAKKVKYK
                     SLTRYVLLSVCISTVVNLLTISPLRNSDSFVTEGQWLTFKSIIALLILCGVVLAINLF
                     FLRFSKRYAFLGRLFLQEIDLFFSSENALSTFFAKPLWLRLFILLVIEMMWITLVSVL
                     ATLVEWRIWFEAYFLLCYVPCLIYYFFHCRFLWHNDFMMACDMYFRWGHFNK"
     gene            complement(4303..4439)
                     /locus_tag="ACHKTW_004510"
     ncRNA           complement(4303..4439)
                     /ncRNA_class="other"
                     /locus_tag="ACHKTW_004510"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(4484..4571)
                     /locus_tag="ACHKTW_004511"
     tRNA            complement(4484..4571)
                     /locus_tag="ACHKTW_004511"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4535..4537),aa:Ser,seq:gga)
     gene            4805..5743
                     /gene="ghrA"
                     /locus_tag="ACHKTW_004512"
     CDS             4805..5743
                     /gene="ghrA"
                     /locus_tag="ACHKTW_004512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016618 - hydroxypyruvate reductase
                     [NAD(P)H] activity [Evidence IEA];
                     GO_function: GO:0030267 - glyoxylate reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
                     /protein_id="HFO6465417.1"
                     /translation="MDIIFYHPTFDTQWWIEALRKAIPQARVRAWKSGDNDSADYALV
                     WHPPVEMLAGRDLKAVFALGAGVDSILSKLQAHPEMLNPSVPLFRLEDTGMGEQMQEY
                     AVSQVLHWFRRFDDYRIQQNSSHWQPLPEYHREDFTIGILGAGVLGSKVAQSLQTWRF
                     PLRCWSRTRKSWPGVQSFSGREELSAFLSQCRVLINLLPNTPETVGIINQQLLEKLPD
                     GAYLLNLARGVHVVEDDLLAALDSGKVKGAMLDVFNREPLPPESPLWQHPRVTITPHV
                     AAITRPAEAVEYISRTIAQLEKGERVCGQVDRARGY"
     gene            5798..6535
                     /gene="ycdX"
                     /locus_tag="ACHKTW_004513"
     CDS             5798..6535
                     /gene="ycdX"
                     /locus_tag="ACHKTW_004513"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HFO6465418.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMIATIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPPER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            6559..7113
                     /gene="ycdY"
                     /locus_tag="ACHKTW_004514"
     CDS             6559..7113
                     /gene="ycdY"
                     /locus_tag="ACHKTW_004514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HFO6465419.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMAQVSADYNALFIGDECAVPPYRSAWVEDATEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            7215..7706
                     /gene="ycdZ"
                     /locus_tag="ACHKTW_004515"
     CDS             7215..7706
                     /gene="ycdZ"
                     /locus_tag="ACHKTW_004515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HFO6465420.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(7770..8603)
                     /gene="csgG"
                     /locus_tag="ACHKTW_004516"
     CDS             complement(7770..8603)
                     /gene="csgG"
                     /locus_tag="ACHKTW_004516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
                     /protein_id="HFO6465421.1"
                     /translation="MQRLFLLVAVMLLSGCLTAPPKEAARPTLMPRAQSYKDLTHLPA
                     PTGKIFVSVYNIQDETGQFKPYPASNFSTAVPQSATAMLVTALKDSRWFIPLERQGLQ
                     NLLNERKIIRAAQENGTVAINNRIPLQSLTAANIMVEGSIIGYESNVKSGGVGARYFG
                     IGADTQYQLDQIAVNLRVVNVSTGEILSSVNTSKTILSYEVQAGVFRFIDYQRLLEGE
                     VGYTSNEPVMLCLMSAIETGVIFLINDGIDRGLWDLQNKAERQNDILVKYRHMSVPPE
                     S"
     gene            complement(8630..9046)
                     /gene="csgF"
                     /locus_tag="ACHKTW_004517"
     CDS             complement(8630..9046)
                     /gene="csgF"
                     /locus_tag="ACHKTW_004517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HFO6465422.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            complement(9071..9460)
                     /gene="csgE"
                     /locus_tag="ACHKTW_004518"
     CDS             complement(9071..9460)
                     /gene="csgE"
                     /locus_tag="ACHKTW_004518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HFO6465423.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDFEKTVVFAL
                     IQTEEALNRRQINQALLSTGDLAHDEF"
     gene            complement(9465..10115)
                     /gene="csgD"
                     /locus_tag="ACHKTW_004519"
     CDS             complement(9465..10115)
                     /gene="csgD"
                     /locus_tag="ACHKTW_004519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm master transcriptional regulator CsgD"
                     /protein_id="HFO6465424.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RSLDDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPEDYPYRDIENWP
                     HINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYNSTESALLTHRE
                     KEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAVSWANDNLRR"
BASE COUNT         2512 a         2282 c         2505 g         2910 t
ORIGIN      
        1 tactgtatta gtgagcttat tgtttgggct tgctttggtt ttggtgaata gttggtttaa
       61 tcagccaggc gttgaggaag ttgttccacg atcaacctat ctgatggtaa tgattgcttt
      121 attttttatc gatactgttg catttatttt tatgcagttg tatttcattt atgaccgtag
      181 gcaattttca aactgtatac ttagtttggc ttttctgagt tgtttgattt actttgttaa
      241 aaccgtcatt atcattcagc aaattattga ggggcgtttg acaagcagtg ttgtccaaaa
      301 tgacattgca atctattatt tgtttcgtca gatgagtttg tgcatattaa tatttctggc
      361 attggtgaat aaagttagtg aaaacacaaa acagcgcaat ttattttcca aaaaaatgac
      421 tttgtgcata agtttgtttt ttgtttttgg ggggccgata gttgctcaca tactttctag
      481 tcattatgag agctacaatt tacacattgc cgagttaacc aatgaaaatg gtcaggtggt
      541 ttggaaagcc tcatatgtta ccataatgat tttcatgtgg ttaaccttgc tttcggttaa
      601 tctatacttt aatggtttac gctatgatat ctggaatgga gtgacggtga ttgctttttg
      661 tgccgtactg tacaatatct ctctgttatt tatgagtagg tatagcgtgt ccacttggta
      721 tattagtcgc actattgaag tagtcagtaa gttaaccgtt atggtaatat ttatgtgcca
      781 tatattcagt gcgctacgag taacaaagaa cattgcacat cgcgatccct taaccaatat
      841 atttaacaga aattattttt ttaatgaact gacagttcaa tcagcatcag ccaaaaaaac
      901 gccttattgc gtcatgatta tggatatcga ccacttcaaa aaagtcaacg acacctgggg
      961 gcatccggtt ggcgatcagg tgataaaaac agtggtgaat atcattggga aaagcatacg
     1021 accagatgac cttttagcgc gcgtcggcgg cgaagagttt ggcgtcttgc tgacggacat
     1081 cgatactgaa cgtgcgaaag ctttagcgga aaggattcgg gaaaatgttg agcgtttaac
     1141 tggcgataat cctgaatacg ctataccaca aaaagtgacg attagtattg gcgctgttgt
     1201 gactcaggaa aatgcgttaa acccgaatga gatctatcga ctggctgata acgcacttta
     1261 cgaggcgaaa gagaccgggc gtaataaggt ggttgtgagg gatgtggtga atttttgtga
     1321 gtcaccataa agcggcattt tgatcctacc cacgtaatat ggacacaggc ctaagcgagg
     1381 ttcttgtttt caaattgttc cggactgagg ccgccacacc aactgtgccg ccgccaccga
     1441 ttgtaatcac attcgatata attaaacacc gttgcccgca ttatttcccg gctgataaag
     1501 tgttctccat ggatacattc cactttcagc gaatgaaaga agctttccac gcaggcatta
     1561 tcgtagcagc aaccttttgc gctcatactt ccacgcagat tatgccgctt cagttgcgcc
     1621 tgataatctg ctgaacagta ctggcctcca cggtccgtgt gaacgataac gttccggggc
     1681 ctcttacgcc gccacagcgc catctgcagg gcatcgcagg ccagttgcgc cgtcatgcgt
     1741 ggcgacattg accagccaat aacggcacgt gaccacaggt caatgaccac tgccagatac
     1801 agccagcctt catctgtacg taagtacgtg atgtctcctg cccacttctg gttcgggcca
     1861 ctggcgtaaa aatcctgctc caacagattt tctgacacag gcaggccgtg tgcgcggtag
     1921 ctgaccgggc tgaacttccg ggaggccttt gccctcagtc cctgacggcg caggcttgcc
     1981 gccacggttt ttacgttaaa ggggtaaccc tgagcacgca gttcatccgt caggcgtggg
     2041 gcaccgtaac gctgttttga ccgggtaaaa gccgcgagga caacgctgtc gcagtgttgg
     2101 cggaactgct gacgcgtgct tatccttgtc cgccgctgac accacgtata ccagccgctg
     2161 cgggccaccc ggagcacgcg gcacattgct ttgatgctga actcagcctg atgtttttca
     2221 ataaagacat acttcatttc aggcgcttcg cgaagtatgt cgcggccttt tggaggatag
     2281 ccagctcttc atcccgttct gccagctggc gtttgagacg tgcaatctcg gtagacatct
     2341 ccagttcacg ttcagaagac gtctgctgat tttgctgttt actgcgccag ttgtagcgct
     2401 gtgattcata caggctgagt tcacgggctg cggcagtaac accgatgcgt tcagcaagct
     2461 tcagggcttc actgcgaaat tcaggcgaat gctgtttacg gggtttttta ctggttgata
     2521 ctgtttttgt catgtgagtc acctctgact gagagtttac tcacttagcc gcgtgtccac
     2581 tattgctggg taagatcagg cggttaaagg catcaaccgc gaacaactgc agacgatgat
     2641 tacggaatca ggcattgcgc tggatattag ctgtgataac acgccgcgcc agcaggttat
     2701 tgggggaacg caggctgcgt tgaatgaatt tgccacgctg ctgatggccg ctggatacga
     2761 accggttaaa ctcggtgtga gcggtgcctg gcacacgcgt ttgatggaag acggcgttca
     2821 ggcaatgcgc gattatcttg ccggactgga tatcgcttcg ccagagcatc aggttctgat
     2881 gaacgtaaca gcaaaaagcg aggtcgctcc gtcaattatc aaagaaaatc tctcattaca
     2941 cttaactcat acggtgaaat ggactgaatc tttcgataca tttctgaata tgccaacccc
     3001 ggtagctttc cttgaaataa gtaataagcc ctatttgggg aatatgttaa atgattttgc
     3061 tggcgtggat cagcaacgag ttatgcattg tcgtaaagca tttagtgatg caaaggtatt
     3121 caaatgattc ctgattattt aacttttatt cgctttcagg ataaacgaaa tctgatatac
     3181 atttatgcta ttggacttat tctgataggc ttttattgga agaatgcagg gtttactttt
     3241 ccatcagagg atattggtgt agttagtggg attctggcct tggtgctgta taattttatt
     3301 tttgatctca aggcgtactg ggcttataaa tgcgtcacga agaatatcga tttttcgtgg
     3361 tttaagaaaa agcagaacca caaaatagaa ttatttctta cacaacctct ggtggcagga
     3421 tttctgtcgt taatcatgtt gagtgcaatg agttgggggc tataccagct tctaccctcg
     3481 ttatatgcgc tgttcctgat ttcgttactt gggccgttgg tcatctttct gctgtttcgg
     3541 atgatccgca ccagttatgt caagcaggtc gctatttcag tagcgaaaaa agtaaaatat
     3601 aaaagtctga ctcgctatgt gctgctttcg gtgtgcatct caacggttgt taacctgctt
     3661 actatcagcc cgttgcgtaa cagtgattct tttgtgacag aggggcagtg gttaacgttt
     3721 aaatcgataa ttgcattgct cattctttgt ggcgtagtgt tggcgattaa tctgtttttt
     3781 ctgcgcttct ccaagcggta cgcttttctg ggcaggcttt ttttgcagga aatcgatctg
     3841 tttttctcca gtgaaaatgc gttgtcgacc ttttttgcca agccgctttg gcttcggtta
     3901 ttcatattgc tggttattga aatgatgtgg attacgctgg tgtcggtatt ggcaacgctt
     3961 gtggaatggc ggatttggtt tgaagcctat tttttactct gctatgtacc gtgcttaatt
     4021 tactattttt tccattgtcg attcctctgg cataacgatt ttatgatggc atgtgacatg
     4081 tatttccgtt gggggcattt taataagtga ggaagttaca ggaagtgacc agataacgca
     4141 tatatgttct ctactatctt gcgcattttg attgttgact gagtaaccag acagttgatg
     4201 tgcacaattt cccctcgccc taacagacgt gggcgggggc acccggtgtg tgcaatatac
     4261 gaaaaaaaag cccgtacttt cgtacgggct cttctttaaa tatggcggtg agggggggat
     4321 tgactcgctt cgctcgccct gcgggcagcc cgctcactgc gttcacggtc tgtccaactg
     4381 gctgtcgcca gttgtcgaac cccggtcggg gcttctcatc cccccggtgt gtgcaatata
     4441 cgaaaaaaaa gcccgtactt tcgtacgagc tcttctttaa agatggcggt gaggggggga
     4501 ttcgaacccc cgatacgttg ccgtatacac actttccagg cgtgctcctt cagccactcg
     4561 gacacctcac caaattgtcg ttcctgtctt gctggaacgg gcgctaattt agggaaatca
     4621 cgacctgagg tcaacaaact ttttgaaaaa atcgcgcgtt tattcaaact tcaatcaatg
     4681 tgtggtttta ataagcgaaa tctgcttttt ttgccaccga ccacggattt gttatgctgg
     4741 tggcctttgt agatcataac gataagtgcg aataaatttc gcacaacgct tttcgggagt
     4801 cagtatggat atcatctttt atcacccaac gttcgatacc caatggtgga ttgaggcact
     4861 gcgcaaagct attcctcagg caagagtcag agcatggaaa agcggagata atgactctgc
     4921 tgattatgct ttagtctggc atcctcctgt tgaaatgctg gcagggcgcg atcttaaagc
     4981 ggtgttcgca ctcggggccg gtgttgattc tattttgagc aagctacagg cacaccctga
     5041 aatgctgaac ccttctgttc cactttttcg cctggaagat accggtatgg gcgagcaaat
     5101 gcaggaatat gctgtcagtc aggtgctgca ttggtttcga cgttttgacg attatcgcat
     5161 ccagcaaaat agttcgcatt ggcaaccgct gcctgaatat catcgggaag attttaccat
     5221 cggcattttg ggcgcaggcg tactgggcag taaagttgct cagagtctgc aaacctggcg
     5281 ctttccgctg cgttgctgga gtcgaacccg taaatcgtgg cctggcgtgc aaagcttttc
     5341 cggacgggaa gaactgtctg catttctgag ccaatgtcgg gtattgatta atttgttacc
     5401 gaatacccct gaaaccgtcg gcattattaa tcaacaatta ctcgaaaaat taccggatgg
     5461 cgcgtatctc ctcaacctgg cgcgtggtgt tcatgttgtg gaagatgacc tgctcgcggc
     5521 gctggatagc ggcaaagtta aaggcgcaat gttggatgtt tttaatcgtg aacccttacc
     5581 gcctgaaagt ccgctctggc aacatccacg cgtgacgata acaccacatg tcgccgcgat
     5641 tacccgtccc gctgaagctg tggagtacat ttctcgcacc attgcccagc tcgaaaaagg
     5701 ggagagggtc tgcgggcaag tcgaccgcgc acgcggctac taataaagca tcaggattcc
     5761 tgctatcctt ggcgggaatt gaatacagga gagagttatg tatcccgtcg accttcatat
     5821 gcataccgtt gccagcacac atgcatatag cacattgagt gattacattg cccaggccaa
     5881 acaaaagggc attaaacttt ttgcgatcac cgatcatggc ccggatatgg aagatgctcc
     5941 gcatcactgg cacttcatta acatgcgtat ctggccgcga gtggttgatg gggtagggat
     6001 cctgcgcggc atcgaagcta acattaaaaa tgttgatggt gaaattgatt gcagcggtaa
     6061 aatgtttgac tcgctggatc tgattattgc cggttttcat gagccggttt ttgcaccaca
     6121 tgacaaagcg accaatacac aagcgatgat cgccactatc gccagcggca atgtgcatat
     6181 aataagccat cccggaaatc ccaaatatga aatagatgtg aaagccgttg ctgaagcagc
     6241 cgcgaaacat caggtggcgc tggaaatcaa taattcttca tttttacact cacgtaaggg
     6301 cagtgaagac aactgtcgtg cggtagccgc agcggtacgt gatgctggag gttgggtggc
     6361 attaggctcg gattctcaca ccgcgtttac catgggggaa tttgaagagt gtcttaaaat
     6421 cctcgacgcg gtagattttc cgccagagcg cattttgaat gtttctccgc gccgcttact
     6481 gaacttcctt gaatctcgag gtatggcacc gattgcggaa tttgcagacc tttaattact
     6541 agtaatggaa atatattaat gaacgagttt tctatcctct gtcgtgtgct gggttcgctc
     6601 tattaccgcc aaccgcaaga tcctttactg gtgccgctgt ttaccctgat tcgtgagggg
     6661 aaactggctg cgaactggcc actggagcag gatgagttac tgacgcgttt acagaaaagc
     6721 tgtgatatgg cgcaagtctc tgccgattac aatgcgttgt ttatcggcga tgaatgtgcc
     6781 gtgccgccat atcgtagcgc atgggttgag gacgcaacgg aagcggaagt gcgcgctttt
     6841 ctttccgaac gagggatgcc actggcggat acgccagccg atcacatcgg cacattgctg
     6901 cttgcggctt cctggctgga agatcagtca acggaagatg agagcgaagc actggaaaca
     6961 ctgttcagtg agtatctgtt accctggtgt ggtgcgttcc tcggcaaagt ggaggcccat
     7021 gcaaccacgc ctttctggcg caccatggca ccgctaaccc gtgatgccat tagtgcaatg
     7081 tgggacgagc tggaagaaga ttctgaagag taaatgtgat catcgtcact cataactgca
     7141 acagataatt tgttgttgca taaaatgtgt gctcgatctc attcatggcc gcgttttctg
     7201 ctatcatgcg cggcatgaac atacttctct ctattgcaat cacaacaggc attctctccg
     7261 gtatctgggg atgggtggct gtttccctgg gcttactaag ctgggcgggc ttcctgggct
     7321 gtacggccta ctttgcctgc ccgcaaggtg ggctgaaagg gctggcgatc tccgctgcaa
     7381 ccctgcttag tggcgtggtg tgggcgatgg tcattattta cggtagtgca ctggcaccac
     7441 atctggaaat tctcggttat gtcataaccg gtattgtcgc gtttctgatg tgtattcagg
     7501 ccaaacagct gctgctttca tttgttccgg gaacgtttat aggcgcatgc gcgacgtttg
     7561 ccgggcaggg tgactggaaa ctggtgttac cttcgctggc gcttgggctg gtatttggtt
     7621 acgcaatgaa aaacagcggc ctgtggctgg cggcgcgtag tgcaaagacg gcgcaccgtg
     7681 agcaggaaat caaaaataaa gcgtgagggg cactcacgct ttcgcttaaa cagtaaaatg
     7741 ccggatgata attccggctt ttttatctgt caggattccg gtggaaccga catatggcgg
     7801 tatttcacca gaatgtcgtt ctgccgttct gctttgttct gcaaatccca cagaccacgg
     7861 tcgataccat cattaatcag gaaaatgacc cctgtttcga tagccgacat cagacacagc
     7921 ataacgggtt cgtttgaggt gtaacccact tccccttcca gcaagcgctg gtagtcaata
     7981 aagcggaaaa ccccggcctg aacttcatag gaaagtatcg tcttactggt gttcaccgag
     8041 gagaggatct caccggtact cacattgacg acacgcaggt tcacggcaat ctgatcgagc
     8101 tggtattgcg tgtctgcacc gatcccaaaa tatcttgccc caacaccgcc cgatttaacg
     8161 ttgctttcat aaccgataat cgaaccttca accataatat ttgccgccgt taaagattgc
     8221 agcgggattc ggttattaat agcaaccgtg ccgttttctt gtgccgcacg aataatcttg
     8281 cgctcgttaa gcaggttttg taagccctgg cgctccagcg gtataaacca gcgagaatct
     8341 ttcaatgccg tgaccagcat tgccgtggcg ctttgcggaa cagcagtgga gaagttactt
     8401 gccgggtagg gtttaaattg cccggtttcg tcctgaatgt tgtataccga aacaaagatc
     8461 ttacccgtcg gcgctggcag atgggtcaaa tctttgtagc tctgagctcg aggcattaat
     8521 gtcggtctgg cggcttcttt aggcggggcg gttaagcatc cgctcagtaa catgacggca
     8581 accaaaagaa ataagcgctg catgattatt ttccttatga agctggggct taaaaatcgg
     8641 ttgagttatt ttgtaaaccc gaaacctgga tggtcgaggt ttgtccggtt ttacgatctg
     8701 tcacgttcaa ctgcaattga ccatcgcggt tagcaatatc aacaatataa tcgttggtca
     8761 ccatgcggcc cggtttaccg gtattaatat tcgacagtag cccacctaaa atttgtgact
     8821 ggatggcctg agtaaagtta tctaacgctg agggtgtttc aataccaaag tcatcgttat
     8881 agctcggatc tttataagag ttttgggcct gagcgctatt taataaaaaa gcgccattat
     8941 ttgggttacc accaaagttt ggattacgga actggaaagt catggttcca gcccaactta
     9001 atggcgaaat aagcatgagt agaactactg catgtttgac acgcattgca gcctccgaac
     9061 aattttttat ttagaattca tcatgcgcca aatcgcccgt acttaataac gcctgattta
     9121 tctggcgacg atttagtgct tcttcagttt gaatcagtgc aaagacgaca gttttctcga
     9181 agtctctttt caacggaaat aaaaaagtct ggaaaataac gtcctgattg accgttatag
     9241 tgatccagct tccccatcgt gcactgggcc tttcattaat cgttaagtta cccgtatagt
     9301 cactttccca tttatcacta aaggctcggt aaaaatcatg gccaatagat gaaacagtat
     9361 ggtcagttaa caatcccggg acttctacct caacggcgtg aagattccct gcggcgaaca
     9421 gaaattctgc cgccacaatc cagcgtaaat aacgtttcat ggctttatcg cctgaggtta
     9481 tcgtttgccc aggaaaccgc ttgtgtccgg ttttttacgg ctatcttctt gaaaagatta
     9541 taaagatgcg ttttaaccgt attttcgctg atgaacaacg aacgagcgat ctcgttatta
     9601 gacgcgccga tacgcagctt attcaggatc tctttttccc gatgagtaag gagggctgat
     9661 tccgtgctgt tataacgata gttaccagaa tgtgtaatca ggtaactggc aagcttttgc
     9721 gtaaagtagc attcgccgcg caggacgcct tgcaacccat tgacaacacg ttcttgatcc
     9781 tccatggcat aaaaaacgcc gttgatatga ggccagtttt caatgtcgcg gtacgggtaa
     9841 tcttcaggcg tatttagcaa caatattttg atattgttgt ttttcctgct caaagtatcc
     9901 tgccaataat ggataagctt tttatctgct tccatcatat ccagaagaat aatagagcct
     9961 gaagatatat cgtccagaga acgttgaata ttatgtaatt ttcctgtaat tgccagcgat
    10021 tgtttaaggt gctgcaagag agctgtcgct tgcaaagacg gtttagtgat caacaataat
    10081 gtatgaccat gaatactatg gacttcatta aacatgatga aaccccgctt tttttattga
    10141 tcgcacacct gacagctgcc tctaaaatag aagcaccaga agtactgaca gatgttgcac
    10201 tgctgtgtg
//
LOCUS       DAZFQD010000169        10164 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.169, whole genome shotgun sequence.
ACCESSION   DAZFQD010000169 DAZFQD010000000
VERSION     DAZFQD010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10164
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.169"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            300..1277
                     /gene="glaH"
                     /locus_tag="ACHKTW_004520"
     CDS             300..1277
                     /gene="glaH"
                     /locus_tag="ACHKTW_004520"
                     /EC_number="1.14.11.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050498 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, with 2-oxoglutarate as one donor, and
                     the other dehydrogenated [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutarate dioxygenase GlaH"
                     /protein_id="HFO6465425.1"
                     /translation="MNALTAVHNNAVDSGQDYSGFTLIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGI
                     DDVAQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDEQNMQGGNSLLLHLDDWEHLDHYFRHPLARRPMRFAAPP
                     SKNVSKDVFHPVFDVDQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGILSVP
                     VPVGKFLLINNLFWLHGRDRFTPHPDLRRELMRQRGYFAYATHHYQTHQ"
     gene            1296..2564
                     /gene="lhgO"
                     /locus_tag="ACHKTW_004521"
     CDS             1296..2564
                     /gene="lhgO"
                     /locus_tag="ACHKTW_004521"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HFO6465426.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNGIRYDNCGKMLVATSELEMERM
                     RALWERTAANGIEREWLNAVELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEIIYNAEVSALSEHKNGVVVRTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILGSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFNQ"
     gene            2587..4035
                     /gene="gabD"
                     /locus_tag="ACHKTW_004522"
     CDS             2587..4035
                     /gene="gabD"
                     /locus_tag="ACHKTW_004522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009013 - succinate-semialdehyde
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HFO6465427.1"
                     /translation="MKLNDSKLFRQQALINGEWLDANNGEVIDVTNPANGDKLGSVPK
                     MGADETRAAIDAANRALPAWRALTAKERANILRNWFNLLMEHQDDLARLMTLEQGKPL
                     AEAKGEISYAASFIEWFAEEGKRIYGDTIPGHQADKRLIVIKQPIGVTAAITPWNFPA
                     AMITRKAGPALAAGCTMVLKPASQTPFSALALAELAIRAGIPAGVFNVVTGSAGAVGN
                     ELTSNPLVRKLSFTGSTEIGRQLMEQCAKDIKKVSLELGGNAPFIVFDDADLDKAVEG
                     ALSSKFRNAGQTCVCANRLYVQDGVYDRFAEKLQQAVSKLHIGDGLEKGVTIGPLIDE
                     KAVAKVEEHIADALEKGARVVCGGKADERGGNFFQPTILVDVPANAKVSKEETFGPLA
                     PLFRFKDEADVIAQANDTEFGLAAYFYARDLSRVFRVGEALEYGIVGINTGIISNEVA
                     PFGGIKASGLGREGSKYGIEDYLEIKYMCIGL"
     gene            4049..5329
                     /gene="gabT"
                     /locus_tag="ACHKTW_004523"
     CDS             4049..5329
                     /gene="gabT"
                     /locus_tag="ACHKTW_004523"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HFO6465428.1"
                     /translation="MSSNKELMQRRSQAIPRGVGQIHPIFADRAENCRVWDVEGREYL
                     DFAGGIAVLNTGHLHPKVVAAVEAQLKKLSHTCFQVLAYEPYLELCEIMNQKVPGNFA
                     KKTLLVTTGSEAVENAVKIARAATKRSGTIAFSGAYHGRTHYTLALTGKVNPYSAGMG
                     LMPGHVYRALYPCPLHGISEDDAIASIHRIFKNDAAPEDIAAIVIEPVQGEGGFYASS
                     PAFMQRLRALCDEHGIMLIADEVQSGAGRTGTLFAMEQMGVAPDLTTFAKSIAGGFPL
                     AGVTGRAEVMDAVAPGGLGGTYAGNPIACVAALEVLKVFEQENLLQKANDLGQKLKDG
                     LLAIAEKHPEIGDVRGLGAMIAIELFEDGDHNKPDAKLTAEIVARARDKGLILLSCGP
                     YYNVLRILVPLTIEDAQIRQGLEIISQCFDEAKQ"
     gene            5567..6967
                     /gene="gabP"
                     /locus_tag="ACHKTW_004524"
     CDS             5567..6967
                     /gene="gabP"
                     /locus_tag="ACHKTW_004524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015185 - gamma-aminobutyric acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HFO6465429.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAG
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYAEVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDTPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLYPWLTWLVIGFITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMAR
                     WKKLVLWQKTPVHNTR"
     gene            6988..7650
                     /gene="csiR"
                     /locus_tag="ACHKTW_004525"
     CDS             6988..7650
                     /gene="csiR"
                     /locus_tag="ACHKTW_004525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HFO6465430.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLR
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLARDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(7651..8100)
                     /gene="kbp"
                     /locus_tag="ACHKTW_004526"
     CDS             complement(7651..8100)
                     /gene="kbp"
                     /locus_tag="ACHKTW_004526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HFO6465431.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLSKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(8184..8342)
                     /locus_tag="ACHKTW_004527"
     CDS             complement(8184..8342)
                     /locus_tag="ACHKTW_004527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HFO6465432.1"
                     /translation="MGFWRIVITIILPPLGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
     gene            8525..8824
                     /locus_tag="ACHKTW_004528"
     CDS             8525..8824
                     /locus_tag="ACHKTW_004528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFO6465433.1"
                     /translation="MTELAQLQASAEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLIDSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
     gene            8834..9358
                     /gene="ygaP"
                     /locus_tag="ACHKTW_004529"
     CDS             8834..9358
                     /gene="ygaP"
                     /locus_tag="ACHKTW_004529"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001461399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HFO6465434.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLSPLS
                     VLEQSGLPPKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKRAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILIGVVLGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQRT"
     gene            complement(9405..9809)
                     /gene="stpA"
                     /locus_tag="ACHKTW_004530"
     CDS             complement(9405..9809)
                     /gene="stpA"
                     /locus_tag="ACHKTW_004530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HFO6465435.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
BASE COUNT         2349 a         2599 c         2748 g         2468 t
ORIGIN      
        1 taatatgttt agtttactaa cggtcatttt gcagtgaagc catttactgt tttttatcga
       61 ccagataatc tgttctctaa tgttaactcc ccctaacctg ttgctttagt tattcatttc
      121 ctgtctcact ttgccttaat accctacgtt aaatgttact aatttgttgc ttttgatcac
      181 aataagaaaa caatatgtcg cttttgtgcg catttttcag aaatgtagat atttttagat
      241 tatggctacg aaatgagcat cgccatgtca ccctacatct cataagagga tcgcttctga
      301 tgaatgcact gaccgccgta cacaataacg ctgtcgattc aggccaggac tatagcggat
      361 tcactctcat cccgtcggcg caatccccgc gtctgctgga actcaccttc accgaacaga
      421 cgaccaaaca gtttctcgaa caggttgccg aatggcccgt gcaggcgctg gagtacaaat
      481 cgtttctgcg ttttcgggta ggcaaaattc ttgacgatct gtgtgcgaat cagctgcaac
      541 cattgctgtt gaagaccctg ctaaaccgtg ctgaaggtgc gctgttgatc aatgcggtgg
      601 gtatcgatga tgtggcgcag gcagatgaga tggtgaagct ggcaacggcg gtagcgcatc
      661 tgattggtcg ctcaaatttc gatgcgatga gcggtcagta ttacgcgcgt ttcgtggtga
      721 aaaatgtcga taactcagac agctatctgc gtcagccgca ccgcgtgatg gaactgcaca
      781 acgacggcac gtatgtcgaa gagatcaccg attacgtgct gatgatgaaa atcgatgagc
      841 aaaacatgca gggtggaaat tcgttgctgc tgcatctcga tgactgggaa catctggacc
      901 actatttccg ccatccactg gcgcgtcgcc caatgcgctt tgccgcgccg ccgagcaaaa
      961 acgtcagcaa agatgttttc catccagtgt tcgacgttga tcaacagggt cgcccggtga
     1021 tgcgctatat cgaccagttc gtccagccga aagacttcga agaaggcgtg tggttgagcg
     1081 agctttcgga cgccattgaa accagcaaag gcattctttc tgtacccgtt cccgttggca
     1141 aattcctgtt gataaacaac ctgttctggc tgcacggtcg cgaccgcttt actccgcacc
     1201 cggatctgcg ccgtgaactg atgcgtcagc gcggctactt cgcttacgcc actcaccact
     1261 accagacgca tcagtagcgc aaaggaattg agcggatgta tgattttgtg attattggcg
     1321 gtggcatcat cggcatgtcg accgccatgc aactgattga tgtctacccg gacgcccgca
     1381 ttgcgttgct ggaaaaagag tccggcccgg cctgtcacca gacgggccac aacagcggcg
     1441 tgatccatgc cggggtttat tacacgcccg gtagcctgaa ggcacagttt tgcctggcgg
     1501 gaaaccgcgc cactaaagcc ttttgcgatc aaaacggcat tcgctatgac aactgcggca
     1561 agatgctggt cgccacctct gaactcgaaa tggaacggat gcgcgcgttg tgggaacgca
     1621 cggcggcaaa cggtatcgag cgcgagtggt taaacgccgt ggaactgcgc gagcgcgaac
     1681 cgaatatcac cgggcttggc ggcatttttg tgccgtccag cggcattgtc agctaccgcg
     1741 aagtcacagc ggcgatggca aaaattttcc aggccagagg cggtgagatt atttataacg
     1801 ccgaagtcag cgccctcagt gagcataaaa acggcgtggt cgtccgaacc cgtcagggcg
     1861 gcgaatatga agcatcgacg ctgattagct gttccgggct gatggctgac cggctggtga
     1921 aaatgctcgg cctcgaaccg ggttttatta tctgcccgtt ccgtggcgag tatttccgcc
     1981 ttgcgccgga gcataaccag attgttaacc atctgattta ccccattccc gacccggcaa
     2041 tgccgttttt gggcgttcat ctcacccgca tgatcgacgg cagcgtgacc gtcgggccaa
     2101 acgcggtgct ggctttcaaa cgcgaaggct atcgcaagcg cgacttctca tttagcgaca
     2161 cgctggagat tttgggctcg tcggggattc gccgggtgct gcaaaaccat ctacgctcag
     2221 gactgggcga gatgaaaaac tcgctgtgca aaagcggcta tctgcggctg gtgcaaaagt
     2281 attgtccccg gctttcgtta agcgatctcc agccctggcc cgccggtgtg cgggcgcagg
     2341 cggtatcgcc ggacggcaag ctgattgacg attttctgtt tgtcaccacc ccgcgcacga
     2401 tccacacctg caatgcgccc tccccggcag cgacatcagc aattcctatt ggtgcgcata
     2461 ttgtcagcaa ggtacaaacg ctgttggcaa gccagagtaa ccccggacgc acgctgcgag
     2521 cggcacgtag tgtggatgcc ttacacgccg catttaatca ataacctttg aaaacaggac
     2581 gtaacaatga aacttaacga cagtaaatta ttccgccagc aggcgttgat taacggggaa
     2641 tggctggacg ccaacaatgg cgaggtcatc gatgtcacca atccggcgaa cggcgacaag
     2701 ctgggtagcg tacccaaaat gggcgctgat gaaacccgcg ccgctatcga cgccgccaac
     2761 cgcgctctgc ccgcctggcg tgcgctcacc gccaaagaac gcgccaacat tctgcgcaac
     2821 tggttcaatt tgttgatgga gcatcaggac gatttagcgc gcctgatgac cctagaacag
     2881 ggcaaaccgc tggctgaagc gaaaggcgaa attagctacg ccgcctcctt tattgagtgg
     2941 tttgctgaag aaggcaaacg catttatggc gacaccattc ctggtcatca ggccgataag
     3001 cgcctgattg ttatcaagca gccgattggc gtcaccgcag ctatcacgcc gtggaacttc
     3061 ccggcggcga tgattacccg caaagccggt ccggcgctgg cggcaggctg caccatggtg
     3121 ctgaagcctg ccagtcagac gccgttctct gcgctggcgc tggcggagct ggcgattcgc
     3181 gcgggcattc cggctggggt atttaacgtg gtcaccggtt cggcgggcgc ggtgggtaac
     3241 gaactgacca gcaacccgct ggtgcgcaag ctgtcgttta ccggttcgac cgaaattggc
     3301 cgccagttaa tggaacagtg cgcgaaagac atcaaaaaag tgtcgctgga gctgggcggc
     3361 aacgcgccgt ttatcgtctt tgacgatgcc gacctcgaca aagccgtgga aggtgcgctg
     3421 tcctcgaaat tccgcaacgc cgggcaaacc tgcgtctgcg ccaaccgttt atacgtgcag
     3481 gacggcgtgt atgaccgctt tgccgaaaaa ttgcagcagg cggtgagcaa attgcacatc
     3541 ggcgacgggc tggagaaagg cgtcaccatc gggccgctga tcgatgaaaa agcggtagca
     3601 aaagtggaag agcatattgc cgatgcgctg gagaaaggtg cgcgtgtggt ttgcggcggt
     3661 aaagcggatg aacgcggcgg caacttcttc cagccgacca ttctggtgga cgttccagcc
     3721 aacgccaaag tgtcgaaaga agagacgttc ggccccctcg ccccactgtt ccgttttaaa
     3781 gatgaagccg atgtgattgc gcaagccaat gacaccgagt ttggccttgc cgcctatttc
     3841 tacgcccgtg atttaagccg cgtcttccgc gtgggcgaag cgctggagta cggcatcgtc
     3901 ggcatcaata ccggcatcat ttccaatgaa gtggccccgt tcggcggcat taaagcctcg
     3961 ggtctgggtc gtgaaggttc gaagtatggc atcgaagatt acttagaaat caaatatatg
     4021 tgcatcggtc tttaactgga gaacgcgaat gagcagcaat aaagagttaa tgcagcgccg
     4081 cagtcaggcg attccccgtg gtgttgggca aattcacccg attttcgctg accgcgcgga
     4141 aaactgccgg gtgtgggacg ttgaaggccg tgagtatctt gatttcgcgg gcgggattgc
     4201 ggtgctcaat accgggcact tgcatccgaa agtggtggct gcggtggaag cgcagttgaa
     4261 aaaactgtcg cacacctgct tccaggtgct ggcctacgag ccgtatctgg agctgtgcga
     4321 gattatgaat cagaaggtgc caggcaattt tgccaagaaa acgctgctgg tcaccacagg
     4381 ttctgaagcg gtggaaaacg cggtgaaaat cgcccgcgcc gccaccaaac gtagcggcac
     4441 catcgctttt agcggcgcgt atcacgggcg cacgcattac acgctggcgc tgaccggcaa
     4501 ggtgaatccg tactctgcgg gcatggggct gatgccgggt catgtttatc gcgcgcttta
     4561 tccttgcccg ctgcacggca taagcgagga tgacgctatc gccagcatcc accggatctt
     4621 caaaaatgat gccgcgccgg aagatatcgc cgccatcgtg attgagccgg ttcagggcga
     4681 aggcggtttc tacgcctcgt cgccagcctt tatgcagcgt ttacgcgctc tgtgtgacga
     4741 gcacgggatc atgctgattg ccgatgaagt gcagagcggc gcggggcgta ccggcacgct
     4801 gtttgcgatg gagcagatgg gcgttgcgcc ggatcttacc acctttgcga aatcgatcgc
     4861 gggcggcttc ccgctggcgg gcgtcaccgg gcgcgcggaa gtaatggatg ccgtcgctcc
     4921 aggcggtctg ggcggcacct atgcgggtaa cccgattgcc tgcgtggctg cgctggaagt
     4981 gttgaaggtg tttgagcagg aaaatctgct gcaaaaagcc aacgatctgg ggcagaagtt
     5041 gaaagacgga ttgctggcaa tagccgaaaa acacccggag atcggcgacg tacgcgggct
     5101 gggggcgatg atcgccattg agctgtttga agacggcgat cacaacaagc cggacgccaa
     5161 actcaccgcc gagatcgtgg ctcgcgcccg cgataaaggc ctgattcttc tctcctgcgg
     5221 cccgtattac aacgtgctgc gcatccttgt accgctcacc attgaagacg ctcagatccg
     5281 tcagggtctg gagatcatca gccagtgttt tgatgaggcg aagcagtagc gccgctccta
     5341 tgccggaggc gacgctgcgc gtcttgtccg gcctacgggg atccaggtcg gataaggcgt
     5401 ttacgccgca tccgacaatc tgtacgtgaa caggaagaaa tctatgttgg ccgggtaagg
     5461 cggagccgct ctccggcaaa aagaatcaat aacaattata cgcgtgaccc ggcgcgggaa
     5521 atgtcggggc gctctcccaa gtgacacact ttcgagagga ttcaggatgg ggcaatcatc
     5581 gcaaccacat gagttaggcg gcgggctgaa gtcacgccac gtcaccatgt tgtctattgc
     5641 cggtgttatc ggcgcaagtc tgtttgtcgg ttccagcgtc gccatcgccg aagcgggccc
     5701 ggcggtatta ctggcctatc tgttcgccgg gctactggtg gttatgatta tgcggatgtt
     5761 ggcggaaatg gcggttgcca cgcccgatac cggttcgttt tccacctatg ccgataaagc
     5821 cattggtcgc tgggcgggct ataccatcgg ctggttgtac tggtggtttt gggtactggt
     5881 tatcccgctg gaagccaaca tcgccgccat gatcctgcac tcatgggttc caggcattcc
     5941 catctggtta ttttccctcg tcattaccct cgccttaact ggcagtaact tattaagcgt
     6001 taaaaactac ggcgaatttg agttctggct ggcgctgtgc aaagtcatcg ctatcctggc
     6061 ctttattttc cttggtgcag tcgcaattag cggtttttac ccgtatgccg aagtgagcgg
     6121 gatctcaaga ttgtgggata gcggcggctt tatgcccaac ggtttcggtg cggtattaag
     6181 cgcgatgttg atcaccatgt tctcgtttat gggcgcagaa attgtcacca ttgccgccgc
     6241 ggaatccgac acgccggaaa aacatattgt ccgcgccacc aactcggtta tctggcgtat
     6301 ttctatcttc tatttatgtt ctatttttgt cgtcgtggca ttaattccgt ggaatatgcc
     6361 cggactaaaa gccgtcggtt cttatcgctc ggtactggaa ttgctcaata ttccccatgc
     6421 gaaattaatc atggactgcg tgatattact ttccgtaacc agttgcctga actcggcgct
     6481 gtataccgcg tcaaggatgc tctactcctt aagccgtcgc ggtgatgctc ccgcggtaat
     6541 gggtaaaatc aaccgcagta aaaccccgta cgtggcggtg ttactctcca ccggcgcggc
     6601 attcttaacg gtggtggtga actattacgc gcctgcgaag gtatttaaat ttctgatcga
     6661 cagctccggc gctatcgccc tgctggttta tttagtcatc gccgtttcac agttgcggat
     6721 gcgcaaaatt ctgcgagcag aaggaagcga aattcgcttg cgaatgtggc tttatccgtg
     6781 gctcacctgg ctagtcatcg gctttattac ctttgtgttg gtagtgatgc tattccgtcc
     6841 ggcgcaacag ttagaagtga tctccaccgg cttattagcg atagggatta tctgtaccgt
     6901 gccaattatg gctcgctgga aaaagctggt attgtggcaa aaaacacccg ttcataatac
     6961 gcgctgatca tgatcaggag tcacaccatg accattacgt ctctggatgg ctatcgctgg
     7021 ctgaagaacg atattattcg cggtaatttt caaccggatg aaaaattacg aatgagtttg
     7081 ctgacatcgc gttatgcact tggcgttggg ccgttacggg aagctctttc gcaactggtg
     7141 gcggaacggc tggtcacggt ggtgaatcaa aaagggtatc gggtggcgtc tatgtcagag
     7201 caggagctgc tcgatatttt cgacgcccgc gccaatatgg aagcgatgtt agtgagtctg
     7261 gcgattgccc gcggtggcga tgagtgggag gcagacgttc tcgcaaaagc gcatctgctg
     7321 agtaagcttg aggcctgtga cgccagcgag aaaatgcttg atgagtggga tctgcgtcat
     7381 caggcgtttc atacggcaat tgtggcgggc tgtggttctc actatttgct gcaaatgcgt
     7441 gaacggttgt ttgatctggc ggcgcgctat cgatttatct ggctgcggcg aacggtgctt
     7501 tcggtagaaa tgctggagga taaacacgat cagcaccaga ccctgactgc ggcggtactg
     7561 gcgcgagata ccgcgcgcgc cagtgagtta atgcgccagc atttactgac gccaattccc
     7621 attatccagc aggcgatggc tggcaattaa ctactcttcc ggaatacgca acacttgccc
     7681 cggataaatt ttatccgggc tttttagcat cggtttattc gcttcgaaga ttttattgta
     7741 cagattagcg ttaccgtaga cctgtttgga aatggcactc agagtgtcgc cagacttaac
     7801 ggtataaaac tggctggcag tggctggtgt cgccgttttc acctgatcat caacactggc
     7861 aataccggaa atattcccaa ccgcaacaag gattttctcc ttcgcctcct gactcaggcc
     7921 gtcaccagtg accgtcgctt tgccgtcggc aatttgaatg ttcactttat cggcatcagg
     7981 tataccggtt ttgctcagat gctcctgcac cttcttcgcc tgatcgtctt tatcgtgctg
     8041 acctgtaacc gcgtcccaga gtttttctcc ggcatctttc acaaaattga acagacccat
     8101 agctacctca ttcgttaacg gaaacactca gaaagtgtag cagaggaacg cggcgcgtta
     8161 acgccgcggg ggtgagcaca ggtttaatcg cgggtttgca cccagaacgc gtgaatcaaa
     8221 ccggggatat acccaagcag tgtcagcaga atattaataa tgaacgccca accgaaccct
     8281 ttaccgagca gcacgccgag cggcggcaga atgatggtga tgacgattct ccagaaaccc
     8341 atatgtactc cctataagaa aattactcat tgtttaaaaa gagattttat ctcttaagcg
     8401 tagtaatttt agcggagcct gccagtttca gcttacgctt ttactctcat ttacgctgct
     8461 gaggctggta tatgttgcat ttttagttta gagtttactt aatttagaaa atacttaaat
     8521 aaatatgact gaactcgcgc aattacaggc cagtgccgaa caggcagcgg ccttattgaa
     8581 agcaatgagc catcctaaac gattgctgat tctgtgcatg cttagcggtt cccccggcac
     8641 cagcgcggga gagctgacgc gcattaccgg actgagtgcc tctgcgacat cacagcatct
     8701 cgctcgtatg cgggacgaag ggcttatcga cagccaacgg gatgcccaac gcattctata
     8761 ttccattaaa aatgaggcgg taaatgccat tatcgccacc ctgaaaaatg tctattgtcc
     8821 gtaaggagtc actatggctt tgacaaccat ttcgccgcat gatgcacaag aattaatcgc
     8881 acgcggcgca aagttaatcg atattcgtga cgctgatgaa tatcttcgtg aacatattcc
     8941 tgaagcagat ctgtctccat tatccgtgct ggaacagtca ggtcttccac ctaaattacg
     9001 tcgcgagcaa attatattcc actgccaggc aggtaaacgc accagtaata acgccgataa
     9061 attagcagcg attgccgccc ccgcagaaat atttttactc gaagatggga ttgatggctg
     9121 gaaaagagct ggattgccag tagcggtaaa taaatctcaa cccttgccgt taatgcgcca
     9181 ggtgcagatc gctgcgggtg gtttaatatt aatcggcgtt gtactgggtt ataccgtaaa
     9241 tagcggtttc ttcttattaa gtggctttgt tggtgccggg ttactgtttg caggaatcag
     9301 cggtttttgt ggaatggcaa ggttgttaga taagatgccg tggaaccaac gaacttgaga
     9361 agcgacgccg gacgcgccct agcagcgaca tccggcctca gtaattagat caggaaatcg
     9421 tcgagagatt taccttctgc cagcgcctga gcaattggct tcggtgtacg gccctgaccg
     9481 gtccaggttt tagtttcacc gttaacatcg gtgaatttat atttcgccgg acgcggctgg
     9541 cgttttttac cagcgcgtgg tgcagcagca gagctattac ctaataactc ttccgggtta
     9601 attccgtcag ccttcatcag ctccagccag gtgctaattt tttcctgacg ctcagccagt
     9661 tcacgctgct gctgttcttc ttcttcacgt ctttctttag tgacaaccct gaatttttcg
     9721 agcatttctt caagaacgtc aatggagaat tcgcgagcca tcgcacggag agtgcgaatg
     9781 ttatttaaac tttgtaacat tacggacata ataaagaaaa ccttttaacg ccaaaacaaa
     9841 aaagtatttc gtcgcgtagt gtaagcgatc acttattata tttccacaaa atttaatcta
     9901 tttacagtcc tgcgcgagat tatttcagct gttattttct gaaactctca gaataatttc
     9961 aagattatat attagccatt ttgatgagta atttagatta ttggtgaaca gagtggtgct
    10021 ctctgctcgc taattccgca agcctgacaa aaccgaagtg aataaaaatg caatatgaca
    10081 gaataaaaga aaacacccct cactggtgct ttctgctgtt aatgtacatt ttctggcata
    10141 aaaaatgctg taaatgaccc ggtc
//
LOCUS       DAZFQD010000170         9901 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.170, whole genome shotgun sequence.
ACCESSION   DAZFQD010000170 DAZFQD010000000
VERSION     DAZFQD010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9901
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.170"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            151..366
                     /gene="bdm"
                     /locus_tag="ACHKTW_004531"
     CDS             151..366
                     /gene="bdm"
                     /locus_tag="ACHKTW_004531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-dependent modulation protein"
                     /protein_id="HFO6465436.1"
                     /translation="MFTYYQAENSTAEPALVNAIEQGLRAEHGVVTEDDILMELTKWV
                     EASDNDILSDIYQQTINYVVSGQHPTL"
     gene            468..605
                     /gene="sra"
                     /locus_tag="ACHKTW_004532"
     CDS             468..605
                     /gene="sra"
                     /locus_tag="ACHKTW_004532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary-phase-induced ribosome-associated
                     protein"
                     /protein_id="HFO6465437.1"
                     /translation="MKSNRQARHILGLDHKISNQRKIVTEGDKSSVVNNPTGRKRPAE
                     K"
     gene            762..2459
                     /gene="maeA"
                     /locus_tag="ACHKTW_004533"
     CDS             762..2459
                     /gene="maeA"
                     /locus_tag="ACHKTW_004533"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HFO6465438.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIISQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            2593..3603
                     /gene="adhP"
                     /locus_tag="ACHKTW_004534"
     CDS             2593..3603
                     /gene="adhP"
                     /locus_tag="ACHKTW_004534"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HFO6465439.1"
                     /translation="MKAAVVTKDHHVDVTDKTLRSLKHGEALLKMECCGVCHTDLHVK
                     NGDFGDKTGVILGHEGIGVVAEVGPGVTSLKPGDRASVAWFYEGCGHCEYCNSGNETL
                     CRSVKNAGYSVDGGMAEECIVVADYAVKVPDGLDSAAASSITCAGVTTYKAVKLSKIR
                     PGQWIAIYGLGGLGNLALQYAKNVFNAKVIAIDVNDEQLKLATEMGADLAINSRTEDA
                     AKIVQEKTGGAHAAVVTAVAKAAFNSAVDAVRAGGRVVAVGLPPESMSLDIPRLVLDG
                     IEVVGSLVGTRQDLTEAFQFAAEGKVVPKVALRPLADINTIFTEMEEGKIRGRMVIDF
                     RR"
     gene            3750..4034
                     /locus_tag="ACHKTW_004535"
     CDS             3750..4034
                     /locus_tag="ACHKTW_004535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFO6465440.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVDVSRLRRLVTQ"
     gene            complement(4085..4738)
                     /gene="fdnI"
                     /locus_tag="ACHKTW_004536"
     CDS             complement(4085..4738)
                     /gene="fdnI"
                     /locus_tag="ACHKTW_004536"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HFO6465441.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(4731..5615)
                     /gene="fdnH"
                     /locus_tag="ACHKTW_004537"
     CDS             complement(4731..5615)
                     /gene="fdnH"
                     /locus_tag="ACHKTW_004537"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HFO6465442.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(5628..8675)
                     /gene="fdnG"
                     /locus_tag="ACHKTW_004538"
     CDS             complement(5628..8675)
                     /gene="fdnG"
                     /locus_tag="ACHKTW_004538"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(8088..8090),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HFO6465443.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPEYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGVLRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLAGQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETLAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            8907..9788
                     /gene="yddG"
                     /locus_tag="ACHKTW_004539"
     CDS             8907..9788
                     /gene="yddG"
                     /locus_tag="ACHKTW_004539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415990.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HFO6465444.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEICLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGDNGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGASLWVYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYAAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
BASE COUNT         2407 a         2590 c         2495 g         2409 t
ORIGIN      
        1 tacctatcaa tatcatgagg ttaaaatata aaaatcatca accaagacta atcttaacaa
       61 cgaagcggca aatatttgcc atgctgaatg tgctttataa atctgcgatc cgtagcagac
      121 accataaata cacagacacg gagaatcact atgtttactt attatcaggc agaaaattca
      181 acagcagaac ccgctctggt taatgccatt gagcaaggcc ttcgggcaga gcacggtgtt
      241 gtcactgaag atgacattct catggagctg accaaatggg tggaagcttc tgataacgac
      301 atcctcagtg atatctacca gcaaacgatc aattatgtgg tcagtggcca gcaccctacg
      361 ctttaaggtg ctatgcttga tcggcaacct aatttagggg tttagcacgt gtttcttcgc
      421 tacggcgatg ttgtccttaa aactagcaac aggattgagg agttaaaatg aaatcgaacc
      481 gtcaggcacg tcatattctt ggactggacc ataaaatttc taaccagcgc aaaatagtta
      541 ccgaaggtga caaatccagc gtggtaaata atccaaccgg cagaaaacgc cccgctgaaa
      601 agtaattcat aaccatcagt cctcaatgac gattaaacac cattgcctgc gcaatggtgt
      661 ttttgttttt atctgctcta tacttggggc cgacgccctg gcggtaaagc aaagacgata
      721 aaagcgtgcc agggatggat attcaaaaaa gagtgagtga catggaacca aaaacaaaaa
      781 aacagcgttc gctttatatc ccttacgctg gccctgtact gctggaattt ccgttgttga
      841 ataaaggcag tgccttcagc atggaagaac gccgtaactt caacctgctg gggttactgc
      901 cggaagtggt cgaaaccatc gaagaacaag cggaacgagc atggatccag tatcagggat
      961 tcaaaaccga aatcgacaaa cacatctacc tgcgtaacat ccaggacacc aacgaaaccc
     1021 tcttctaccg tctggtaaac aatcatcttg atgagatgat gcctgttatt tataccccaa
     1081 ccgtcggcgc agcctgtgag cgtttttctg agatctaccg ccgttcacgc ggcgtgttta
     1141 tctcttacca gaaccggcac aatatggacg atattctgca aaacgtgccg aaccataata
     1201 ttaaagtgat tgtggtgact gacggtgaac gtattctggg gcttggtgac cagggcatcg
     1261 gcgggatggg cattccgatc ggtaaactgt cgctctatac cgcctgtggc ggcatcagcc
     1321 cggcgtatac ccttccggtg gtgctggatg tcggaacgaa caaccaacag ctgcttaacg
     1381 atccgctgta tatgggctgg cgtaatccac gtatcactga cgatgaatac tatgaattcg
     1441 ttgatgaatt tatccaggct gtgaaacaac gctggccgga cgtgctgttg cagtttgaag
     1501 actttgctca aaaaaatgcg atgccgttac ttaaccgcta tcgcaatgaa atttgttctt
     1561 ttaacgatga cattcagggc actgcggcag taacagtcgg cacactgatc gcagccagcc
     1621 gcgcggcagg tggtcagtta agcgagaaaa aaatcgtctt ccttggcgca ggttcagcgg
     1681 gatgcggcat tgccgaaatg atcatctccc agacccagcg cgaaggatta agcgaggaag
     1741 cggcgcggca gaaagtcttt atggtcgatc gctttggcct gctgacggac aagatgccga
     1801 acctgctacc tttccagacc aaactggtgc agaagcgcga aaacctcagt gactgggata
     1861 ccgacagcga tgtgttgtca ctgctggatg tggtgcgcaa tgtaaaacca gatattctga
     1921 ttggcgtctc aggacagacc gggctgttta cggaagagat cattcgtgag atgcataaac
     1981 actgtccgcg tccgatcgtg atgccgctgt ctaacccgac gtcacgcgtg gaagccacac
     2041 cgcaggacat tatcgcctgg accgaaggta acgcgctggt cgccactggc agcccgttta
     2101 atccagtggt atggaaagat aaaatctacc ctatcgccca gtgtaacaac gcctttattt
     2161 tcccaggcat cgggctgggt gttattgctt ccggcgcgtc acgtatcacc gatgagatgc
     2221 tgatgtcagc aagtgaaacg cttgctcagt attcgccgct ggtgctgaac ggcgaaggtc
     2281 tggtactacc ggaactgaaa gatattcaga aagtctcccg cgcaattgcg tttgcggttg
     2341 gcaaaatggc gcagcagcaa ggcgtggcgg tgaaaacgtc tgccgaagct ctgcaacaag
     2401 ccattgacga taatttctgg caagccgaat accgcgacta ccgccgtacc tccatctaag
     2461 cctgcgcccg gtagtgaagg ctaccgggct atttccctct ccctttttca gatctcatcc
     2521 atactgggta gtggcgaata aatctcattt gcctcacctg ctatgcagaa catcatccga
     2581 aaaggaggaa ctatgaaggc tgcagttgtt acgaaggatc atcatgttga cgttacggat
     2641 aaaacactgc gctcactgaa acatggcgaa gccctgctga aaatggagtg ttgtggtgta
     2701 tgtcataccg atcttcatgt taagaatggc gattttggtg acaaaactgg ggtaattctg
     2761 ggccatgaag gcatcggtgt ggtggcagaa gtgggtccag gtgtcacctc attaaaacca
     2821 ggcgatcgtg ccagcgtggc gtggttctac gaaggatgcg gtcattgcga atactgtaac
     2881 agtggtaacg aaacgctctg ccgttcagtt aaaaatgccg gatacagcgt tgatggcggg
     2941 atggcggaag agtgcatcgt ggtcgccgat tacgcggtaa aagtgccaga tggtctggac
     3001 tcggcggcgg ccagcagcat tacctgtgcg ggggtcacca cctacaaagc cgttaaactg
     3061 tcaaaaattc gtcccggtca gtggattgct atctacggtc ttggcggtct gggtaacctc
     3121 gccctgcaat acgcgaagaa tgtctttaac gccaaagtga tcgccattga tgtcaatgat
     3181 gagcagttaa aactggcaac cgaaatgggt gcagatttag cgattaactc acgcaccgaa
     3241 gacgccgcca aaattgtgca ggagaaaacc ggtggtgcac acgctgcggt ggtgacagcg
     3301 gtagctaaag ctgcgtttaa ctcggcagtt gatgctgtcc gtgcaggcgg tcgtgttgtg
     3361 gctgtcggtc tgccgccgga gtctatgagc ctggatatcc cacgccttgt gctggatggc
     3421 attgaagtgg tcggttcgct ggtcggcacg cgccaggatc taaccgaagc cttccagttt
     3481 gccgccgaag gcaaagtggt gccgaaagtc gccctgcgtc cgttagcgga catcaacacc
     3541 atctttaccg agatggaaga aggcaaaatc cgtggccgta tggtgattga tttccgccgt
     3601 taagaggcct ttgctgcgag tgccagtcct ggtgtcgcag catcgcactc tctccgacat
     3661 taaatggcta aatggattat tgttccttcc tacgcaacaa taatgatagt gttcctgatg
     3721 aaggaacact actgagagta aagacactga tgaaaatggc caatcatccc cgcccggggg
     3781 acattattca ggaatcactg gacgaactta atgtcagcct gcgcgagttt gccagagcaa
     3841 tggaaattgc gccctcaacg gcaagccgat tgctgaccgg aaaagcagct ttgacaccag
     3901 aaatggcgat taaactttcc gtggtgatcg gcagttcgcc gcaaatgtgg ctgaatctgc
     3961 aaaacgcctg gagtctggca gaggcagaaa aaacggtgga tgtgtcgcga ctacgccgtc
     4021 tggttacgca ataaaaaaat ggcgcttctt aaagcgccat tttcagctta aagttcaaaa
     4081 gagattatat cccttcttca ctctcttttt tcgcttctgc cttctcgatt tcacgatacc
     4141 agcgcggatg gtgtttcttc gcccagcgac ggctcacctt cccttcgatc atccctttaa
     4201 tcgacccttt cacccagaat gccatataca tatggatcag gatggcgtgg atcaggatga
     4261 tacccgcagc cgcgtggatc agcaggctgt agcgaacaac ctgcatcggg aagtactgcg
     4321 caaagtacgg acgccagata atcaccccgg tcaccagcag cacgaaaatc atgctcatga
     4381 tcgaccagaa catcatcttt tgcccggcgt tgtacttacc gacatccgcc actttatgct
     4441 cattgccttt caatacttcg acaatgttca acagccacgg aatatctttc ttatccggga
     4501 tgttgtgatg cacaaaacgc acaaacataa acatcagtgc gacgaaaatc gcaatgccga
     4561 aaaacgggtg caaaatgcgt cccatctgcg gcgtaccgaa ggtttgcgtc agccattgca
     4621 gcgtcgggaa gaagaacgaa atcccggaca gcgccaccag gaagaagcaa atcaccacgg
     4681 tccagtgaca ggcgcgatca ataaatttgg tgcgcacaat cattttcgac ttactcatga
     4741 tgatcctcct cgtcatcgtc cacttcctta ttcgggccaa taccgatgta gtggaaaatc
     4801 aacccggcaa aagtggcaat aaagccagcc gctgccagcg gtttcaacgc gcctttccac
     4861 aggcttaccg aggtgtcgat cttcggatct ttcggcagac cgtgatacag ctccggctga
     4921 tcggcgtgat gcagcacgta cataacgtgc gtaccaccga ccccttccgg gttgtagacg
     4981 ccagcatgtt cgtaaccacg cgctttcagt ttcgccacgc gctgttccgc cagctccagc
     5041 atctccttct tggtgccgaa gtggatagcc ccggtcggac aggttttcac acaagccggt
     5101 tcctggccga cgctgacgcg atcgacgcag agcgtgcatt tatatacccg gttatcctct
     5161 ttgttgaggc gcggaatatt aaacggacac ccggcaatgc agtaaccaca gccgatgcag
     5221 ttttccgact ggaaatcgac aatcccgtta gcgtactgaa tgattgcacc agcagacggg
     5281 cacgccttca ggcagccggg atcttcacag tgcatacagc cgtctttacg gatcagccac
     5341 tccagcttgc cgttctgttc ggtttcgcta aagcgcatca ccgtccagga cttggcgctc
     5401 agatcggcgg ggttatcgta aaccccgacg cagtgcccca cttcatcacg gatgtcgttc
     5461 cactccgaac acgccacctg acaggcttta cagccgatac aggtggaaac gtcgataagt
     5521 tttgcgactt ctgctttgta atcacgcacc tgagaaggcg gcgtgatgga gttagttgcg
     5581 gaccttttga taatgtcctg cgtttccata gccatctgtt cgccccctta cgccttctcg
     5641 atgttgacta agaacgcttt atattccggc gtttgcgagt ttgcatcacc gacattcggc
     5701 gtcagagtgt tagcgatata acctttacgc gcgacaccct caaagcccca gtggattgga
     5761 atacccaccg tttcaacctg ctgaccattt acattcagtg gtttcagacg acgcgttacc
     5821 acagccaccg cgcggataaa gccacgcttg cttgagacag tgacacgatc gccattatta
     5881 atgcctttcg ccgccgccag cgtttcgctg atttccacaa actgttccgg ctgagcaatt
     5941 gcgttgagca atgcgtgctt ggtccaggtg tggaagtgct cggtcagacg ataggtcgta
     6001 cccacatacg ggaactgctc ttttttaccc atccgcagtg cgtcttgttc atacagacga
     6061 acaaccgggt tagacaccac gttcggatgc agcgggttag tgcccagcgg cgtttcaatc
     6121 ggctcgtagt gttccgggaa cggaccttcc gccattttgt tgatggcaaa caggcgtccc
     6181 atcccttccg gctgcatgat aaacggcccg gttggcgtac ccggtgcggc attgccgaag
     6241 tcaggaatat cgttacccgt ccacttgctg ccgttccact ggatcagcat ccgtttcgga
     6301 tcccacggtt taccgttgat atccgccgaa gcacggttgt acagcacgcg acggttgagc
     6361 ggccacgccc aggcccatcc cagcgtattc cccagaccgg acgggtcgga gttatcgcgg
     6421 ttagccatct ggttgccctg ctctgtccag ctaccggtgt agatccagca agaagatgcg
     6481 gttgtaccgt catcacgcag atgcgcaaag ctactcagca actgaccttt cttcgcaatc
     6541 agcacgccat tagcatcata gagatcttcc agcgcatagc cgttgttctc tttagctact
     6601 tcgtcagatt gcggttcgtg cggctgcttg tagttccagc tcatcttcat cagcggttct
     6661 acgcctttac caccttcggc ctggtacagc tcgcgcaggt gatggtagat acccgccaga
     6721 atttcaccgt cgttacgcgc ttcgcccggc gcatcctgac ctttccagtg ccactgcagc
     6781 cagcgaccgg agttagcaat agaaccatct tcttcagcaa agcaggtcga aggcagacgg
     6841 aatacttcag tctgaataga cgccggatcg acatcgttcg actcgccgtg gttctgccag
     6901 aaggttgagg tttcagtcac cagcggatcg ataaccacca tgtacttcag cttgctcagg
     6961 cagctcacca ctttgttttt gtccgggaag gacgcaaccg ggttaaagcc ctggcagaaa
     7021 taaccggtga ctttgccttc atccatcatg ttgaaatact tgatgacgtc gtaggtctgg
     7081 tcccacttcg gcagccagtc atagccccag ttgttctctt tctgcgcggc atcgccatag
     7141 aaagatttca tcaggctaac gaagaacttc ggatagttgc tccagtagtt cacctgacca
     7201 gccagcgtcg ctttcggcgt gttcgcttcc agatacgact gcaaatcaac ctgtttttct
     7261 gacggcagcg tcagataacc tggcaggctg gtagagagca gacctaagtc agtcagaccc
     7321 tgaatgttgg agtgaccacg caatgcgttc acgccgccac cggccatacc catgttaccg
     7381 agcagcaact ggatcatcgc catagtacgg atgttctgcg cacccacggt gtgctgcgtc
     7441 cagcccagcg cgtacaggaa ggtggttgtg cgatccggtg cgctggtgga ggccagcact
     7501 tcacacactt tcaggaagtc ggcttttggc gtaccacaga tgttttcaac aacgtccggc
     7561 gtgtagcggg aaacgtgcgc tttcagcagg ttccacacac agcgcggatg agtcagtgtt
     7621 tcatcgcgtt tcgcatagcc gttttcatcg aactgatagt tccaggacga tttatcgtat
     7681 tgccgttttt cagcgtcgta gccgctgaac agaccgtctt cgaaagcaaa atcatcacgc
     7741 accagcaggc tggcgttggt gtaatgttta acgtattcgg cgttgatttt gttgttttcg
     7801 atcaggtagc gcaaaacgcc agacaggaac gtaatgtccg taccggaacg aataggtgcg
     7861 taaatatccg ccacagaagc ggtacgcgta aaacggggat cgacaacgat caaggttgca
     7921 tcgttgttgt ttttcgcttc catcgcccag cggaaaccga cgggatgcgc ttcagcagcg
     7981 ttaccgccca ttaccattac gacgttagcg tttttgatat ccacccagtg gttggtcatc
     8041 gcaccgcgac caaatgttgg agcaagactt gctaccgttg gtccgtgtca gacgcgcgcc
     8101 tggttgtcta ccgccagcat cccgagggag cgggcaaatt tctgtgtcag catcccggtt
     8161 tcgttgctgg caccggaggc acacagcata ccggtagaaa gccaacggtt taccgttacg
     8221 ccctgctcgt tcttttcaat aaagttagcg tcacggtcag ctttcatcag ctttgcaata
     8281 cgggagaatg cttcttccca gctaatgcgc tgccatttgt cagaacctgg cgcacgatat
     8341 tccgggtagc gcagacggtt ttcgctgttg acgtaatcca gcaaaccggc cccttttggg
     8401 cacagcgcac cacggcttac cggatgatcc gggtcacctt caatgtgata aatcgcttct
     8461 ctggcgtttt tcgcgccatc acccaggcta tacatcaata gcccgcaacc tacggaacag
     8521 tatgtgcagg tgttacggat ctctttagcg cgtaataatt tgtagtttcg cgcctgagcc
     8581 agtgcttgct tcggggcaaa gcccaatgcc gctaccgttg ttccagccat accgcccgcg
     8641 cagattttaa aaaattgtct gcgactgacg tccattgctt tcctcttttt tcaggggtat
     8701 tactgcgcga ggaaactaac agtaaaattc tgaatctttt tgcgcgaaat caaaaagaac
     8761 acgtgtttgc cacaaaataa tccgcctgcg caaattgacc tacctcaata gcggtagaaa
     8821 aacgcaccac tgcctgacag gccagttaaa aaaatgctat aaaattcagc ttaattttta
     8881 acggcaagag agacaaaaca gcgagcatga cacgacaaaa agcaacgctc atagggctaa
     8941 tagcgatcgt cctgtggagc acgatggtag gattgattcg cggtgtcagc gaggggctcg
     9001 gcccggtcgg cggcgcagct gctatctatt cattaagcgg gctgctgtta atcttcacgg
     9061 ttggatttcc gcgtattcgg cagattccga aaggctattt actcgccggg agtctgttat
     9121 tcgtcagcta tgaaatctgt ctggcgcttt ccttagggta tgcggcgacc cgtcatcagg
     9181 cgattgaagt gggtatggtg aactatctat ggcccagcct gacaattctc tttgccattc
     9241 tgtttaatgg tcagaaaacc aactggctga ttgtaccagg attattatta gccctggtcg
     9301 gcgtctgctg ggtgttaggc ggtgataatg ggttacatta tgatgaaatc atcaataata
     9361 ttaccaccag cccattgagt tatttcctgg cgtttattgg tgcgtttatc tgggcagcct
     9421 attgcacagt aacgaataaa tacgcacgcg gatttaacgg aattaccgtt tttgtcctgc
     9481 taacgggagc aagtctgtgg gtttactatt ttctcacgcc acaaccagaa atggtattta
     9541 gcacgcccgt catgattaaa ctcatctctg cggcatttac cttaggattt gcttatgctg
     9601 catggaatgt cggtatactg catggcaatg tcaccataat ggcggtaggc tcgtatttta
     9661 cgcccgtact ttcctcagcg cttgcagccg tgctgctcag cgccccgctg tcgttctcat
     9721 tctggcaagg cgcgctaatg gtctgcggcg gttccctgct ctgctggctg gcgacacgtc
     9781 gtggttaaaa taattgccgg gtctacggcc cggcagttaa tcagataaat ttcgacaata
     9841 ttaaaaaata aatttcaaat accagtaatt caccttatat ctcatttcga cttcgatcac
     9901 a
//
LOCUS       DAZFQD010000171         9726 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.171, whole genome shotgun sequence.
ACCESSION   DAZFQD010000171 DAZFQD010000000
VERSION     DAZFQD010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9726
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.171"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(139..291)
                     /locus_tag="ACHKTW_004540"
                     /pseudo
     CDS             complement(139..291)
                     /locus_tag="ACHKTW_004540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415759.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
     gene            628..2184
                     /locus_tag="ACHKTW_004541"
     CDS             628..2184
                     /locus_tag="ACHKTW_004541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012884439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HFO6465445.1"
                     /translation="MHDYLLFLLGLTPLLAMIILILKVKMPIHYSVLSTLLLTLILGE
                     VFWQTPVMDMGSAVVYGAIKGLWPIVIVILAAIYSYNLMNATKSMDILRNVLASVSDD
                     KRIQVLLISWCFGGFLEAAAGYGTAVAIPIGILIALGFNPLKAAIASLVANTVPTAFG
                     AVGIPVSVLAEQVNLPVTTLSGTIILQLALFNILLPFVIVTIIGGGMKAIRGVGLITL
                     MCGISTLIPQYLVAVNLGAELPAFAGSLVSLIVIVFMAKNRKEKTEPEFLIESSADSA
                     SALSYSRGALLRACSIYILIFLFILLCSPLFPTIKSAVSQIASSLTFSLSNGSNLSMK
                     IEWIATPGVLIIIASVIGGLIQKASLSCLLNVFVSTVKQLKNSIIAISAIVAMATVMD
                     VCGLIGTLAQSLVDLTGGAYLFIAPVIGALGTFVTGSDTNSNILFGKLQTTAAEKLHV
                     DPLWLAAANTSGATGGKMISPQSIAIAVSATRMDGQGSVIMSGTLKYCFAYIVILGLK
                     VGLVYHLFMA"
     gene            2234..2953
                     /locus_tag="ACHKTW_004542"
     CDS             2234..2953
                     /locus_tag="ACHKTW_004542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HFO6465446.1"
                     /translation="MNVNFFVTCIGDAFKSRMAQKSVLLLEQLGCNVHFPEKQGCCGQ
                     PAINGGYVRDAIPGMKNLISSLEENDWPVVSPAGSCTYTIKNYARYLSEEPEWAARAE
                     KVAERLHDLTSFIVNHLGITDVGAKLNGRAVYHPSCSLSRKLGVTREPLLLLENVQGL
                     ELLPFHAQETCCGFGGTFSVKMAEISGEMVKEKVGHIMDVKPDFLIGADASCLLNIGG
                     RLNREGHPVKVMHIAEVLMHR"
     gene            2964..4379
                     /locus_tag="ACHKTW_004543"
     CDS             2964..4379
                     /locus_tag="ACHKTW_004543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HFO6465447.1"
                     /translation="MSLKTSDIRFKERIRIQIQDPIMRKAVANAQERIGTNRQKMVDE
                     LGHWEEWRSRAEQIRRHVLENLDAYLYQLSEKVTANGGHVYFARTREDATSYILKVAK
                     EKNARKVVKSKSMVTEEIGMNAVLQKAGIPVIETDLGEYILQLAHEPPSHVVVPAIHK
                     DRHQIRRVLHEHLGYDGPETPEAMTLFIREKIRQDFLSAEVGVTGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFQEVDVLITMLARSAVGARLTGYNTWLTG
                     PREEGHVDGPEEFHLVIVDNGRSEILGSEFSDVLRCIRCGACINTCPAYRQIGGHGYG
                     SIYPGPIGAVISPLLGGYEDFKDLPYACSLCTACNGVCPVKIPLATLIQKHRQVMAES
                     EITPLTERRITRAFNYINSHPALWKVGMVAGAKTARLFIRKGKTPISVGAIKEWTEAR
                     DLPSPDGESFRSWFKRHKKEG"
     gene            4383..5081
                     /locus_tag="ACHKTW_004544"
     CDS             4383..5081
                     /locus_tag="ACHKTW_004544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000431499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HFO6465448.1"
                     /translation="MENREEFLVAIARKLGRPVRHIPEAMPEPVNTLATTRLTELTSD
                     QRCEAFIKFASEVMLAECVLVSPENAPSTALDICWKFGSGPVIISNDQRLVDTGITPL
                     LQKEMDAALWDPEKGDENIHLAEKAKTGVVFAEYGLTESGGIVLFSAPERGRSVSLLP
                     EASLFVLRKSTILPRVAQLAQKLHDMAQEGQRMPSCINLIGGPSSTADIELIKVVGVH
                     GPVHATYLIIDDCD"
     gene            complement(5486..8740)
                     /locus_tag="ACHKTW_004545"
     CDS             complement(5486..8740)
                     /locus_tag="ACHKTW_004545"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001573238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HFO6465449.1"
                     /translation="MSLSFSEAKLEQAIIELLQDQGYQHLIGDNVPRSSLDQVIIEDD
                     LRHYLAARYQPDGITEEEIQRLIKQFTTLPASDLYESNKTFCRWLANGFLFKRDDRQQ
                     KDLYIELLDTRHLPAALRQIFDAEDVLLQQAAELPPSYINPPLNLIKIVNQLKISGKD
                     NQSRIPDGILYINGLPLVVFEFKSAVREQDASIGNAWRQLCKRYRRDIPQLFIYNALC
                     IISDGVNNRMGNLFAPYEYFYSWRKVTGNENREQDGIPSLHSMIQGLFHPVRLLDVIK
                     NFICFPDKARHEVKICCRYPQYYAARKLYYSIKQARKPFGNGKGGTYFGATGCGKSYT
                     MQFLTRLLMKSVEFASPTIVLITDRTDLDDQLSAQMCNAKNYIGDDTILPVTSREDLR
                     NQLAGRNSGGVFLTTIHKFTEDTELLSERSNIICISDEAHRSQVNLDQKVIVDKESGK
                     VRKTYGFAKYLHDSLPNATYVGFTGTPIDATLDVFGEVIDSYTMTESVQDEITVRIVY
                     EGRAAKVILDSSKLEEVEKYYEECANAGTNEWQIDESKKATATMNAVLGDEDRLKALA
                     EDFAKHYEKRVAEGSTVKGKAMFVCASREIAWDFYRQLKAIRPAWFEVKQAPDGVFLT
                     EQEQKELPPSEMVKMVMTRGKDDDEALYDLLGTKEYRKELDKQFKNAKSNFKIAIVVD
                     MWLTGFDVPELDTIYIDKPLQKHNLIQTISRVNRKLEGKSKGLVVDYIGIKSQMNQAL
                     AMYSRIDATNFEDIQQSVTEVKNHLDLLGQVFYDFDSRDYFSGEPQAQLSCLNRAAEF
                     VLRTQKVERRFMGLVKRMKAAYDVCCGSEALSQTERDHIHYYLAVRSIVFKLTKGDAP
                     DVTQMNARVREMIAEALKADGVEEIYFLGDKKAESIDIFDEDYLARINKIKLPATKIQ
                     LLQKLLEKAISDFRKVNQLQGINFTRRFQAIIDRYNERREDDVLNGEEFDTFSQEMTD
                     IIYDIKTEMGTWADLGIDIEEKAFFDILAHMRDKYQFTYDDEKMLSLAKEMKSVVDNT
                     SKYPDWSKRDDIKAKLKVELILLLHKHKFPPVANDDVYMGVLAQAENFKKNHMS"
     gene            complement(8835..>9726)
                     /locus_tag="ACHKTW_004546"
     CDS             complement(8835..>9726)
                     /locus_tag="ACHKTW_004546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001518290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFO6465450.1"
                     /translation="VINKSLEQMSQTLFKSWFVDFDPVIYNALDAGNPIPEALQSRAE
                     LRQKVRNSTDFKPLPAEIRSLFPSEFEETELGWVPKGWSIVRTEDIALKIGMGPFGSN
                     IKVSTFVNAGVPIISGQHLKALLLIDGDNNFITPEHAEKLKNSAVYRKDIIFTHAGNI
                     GQVSLIPEDSEYDRYIISQRQFFLRVNESKSSPYYLIHYFRSEKGQHALLSNASQVGV
                     PSIAQPSTHLKNISFLNPPMVLLKEFEKFSTPLFHRFSKNRKCGVSLTALRNTLLPKL
                     ISGELSLEDLPDLSTDTEAA"
BASE COUNT         2571 a         2120 c         2260 g         2775 t
ORIGIN      
        1 atgtttttta taaaacttta aattaattgc aaaatggcat ctgaatcgat catagtttgg
       61 cattcgaatc gatcatgatt tggctctctt gctccggctt tatccatttc ctctcagacg
      121 acagtcagtg tcctgctctg actgaacctc tgcaccgttg ttacgtacca gcgtttgttt
      181 atctattaga gtcacttcag taggcacatc agatgccaac gcaacattcc ctgccataag
      241 cgcagccaga acgcctgtta caactaaact attcctggtg atattgctca ttctctttct
      301 ccatttgtcg aggatttggg cgttgatttt cgacgagctc cagttgcaac tccagtctta
      361 aggttatatc gggatagcac acaagtgtta cacacataaa caaaaaatac ccaaaaagta
      421 ttattccata acatttattc atggaagata acaatgaaac gattcagcta tattttttaa
      481 actaaaaaat actttataag aacatcccaa ttttggaggc gatactcatt ctccccctga
      541 taactaaaca ctctcgtccg acagggcgtg gaaactatca ataaccacag taatgatgct
      601 gactttgttt tcgtttaagg aaatgacatg cacgattacc tgcttttttt actgggactt
      661 accccccttc tggcgatgat cattttgatt ctgaaagtca aaatgcccat acactattct
      721 gtgcttagca ctctcctgct cacgctaatc cttggagagg ttttctggca gacgccagtt
      781 atggatatgg gatctgctgt ggtatacggc gcaatcaaag gtttgtggcc gattgtgatt
      841 gtcatccttg ctgccattta cagctataac ctgatgaatg cgacaaaaag catggatatt
      901 ctgagaaatg ttctggcgag cgttagtgac gataaacgca tccaggttct tcttatctca
      961 tggtgttttg gcggatttct tgaggcggcc gccggatacg gtacagcggt agcaatcccc
     1021 ataggtatac tcattgcact gggctttaac ccactaaaag cagcgattgc atcactggtt
     1081 gctaatacag tccctacagc gtttggggca gtcggtatac ctgtctccgt gcttgctgaa
     1141 caagttaatt tacctgtaac cacactcagc gggacgatta ttcttcagct ggcactgttc
     1201 aacattctgc tccccttcgt tatcgtcacg attatcggtg gaggtatgaa agctatacga
     1261 ggcgtagggt taattaccct gatgtgcggt attagcacat tgatacctca gtatctggtg
     1321 gctgttaatc ttggcgcaga gctcccggct ttcgcaggaa gtcttgtcag tctgatcgtt
     1381 atagtcttta tggcaaaaaa ccgtaaagaa aaaacagaac ctgaatttct tattgagagt
     1441 tcagcagata gcgccagcgc actttcatac tctcgtgggg cgttgttacg tgcgtgctca
     1501 atctacattc tgatctttct gttcattctg ctttgctctc cactgttccc gacgataaaa
     1561 agtgcagtgt ctcagattgc ctcgtcactc accttctctc tgagtaacgg cagcaatctg
     1621 tcgatgaaaa ttgaatggat cgcaaccccc ggtgttctga ttatcattgc cagtgttatt
     1681 ggcggcttga tacagaaagc atccctgtca tgcctgttaa acgtatttgt cagcacggta
     1741 aaacaactta aaaactccat tattgccatt tctgcgattg ttgccatggc aacggtaatg
     1801 gatgtctgtg ggttgattgg cactctggca cagagccttg tggatctgac tggtggggcc
     1861 tacctcttca ttgctccggt tatcggcgct cttggtacat ttgttactgg tagtgacaca
     1921 aactccaaca ttctgtttgg taaattgcag accactgcgg ctgaaaaatt gcatgtggac
     1981 ccgctctggc tggctgccgc aaatacatct ggcgcaacag gcggcaaaat gatttctcct
     2041 cagagtattg ctatcgctgt ttcagccacc cgtatggatg gtcagggcag cgtaatcatg
     2101 tcaggaacac tgaagtactg ctttgcttat attgttattc ttggactgaa agtaggcctt
     2161 gtctaccacc tgttcatggc ctgagaaaaa acaacaaaag gacgtggaat attaatcaac
     2221 aggagaaatt tctgtgaatg ttaacttttt cgtcacctgc attggtgatg cgtttaaatc
     2281 ccggatggca caaaagagtg tgctgttact ggagcaattg ggttgcaatg tccatttccc
     2341 cgaaaagcag ggatgttgtg gtcagccagc gataaatggc ggttatgttc gtgatgccat
     2401 cccgggcatg aaaaatctca tatcttcgct ggaagagaat gactggccgg tagtatctcc
     2461 tgccgggtca tgcacttaca cgataaaaaa ttacgctcgc tatctcagtg aagaacctga
     2521 gtgggcggca cgagctgaaa aagtggctga acgcctgcat gatttgacat catttatagt
     2581 aaatcatctt ggcatcacag atgttggggc aaaactcaat ggtcgcgctg tgtaccatcc
     2641 ctcctgcagc ctttcccgta agctgggtgt aaccagagag ccgcttttgc tacttgagaa
     2701 tgtacagggg cttgagctac tgccgtttca cgctcaggaa acgtgctgcg ggtttggcgg
     2761 cacattctct gtaaaaatgg cggagatctc tggtgaaatg gtgaaagaga aagtcgggca
     2821 tattatggat gttaaacctg attttcttat cggggctgac gccagttgcc tgctaaacat
     2881 cggcggtcgg cttaaccgtg aagggcatcc tgtaaaagtg atgcatattg ctgaagtcct
     2941 gatgcacaga tgaggtaaac agaatgtctt tgaaaacaag tgatatcagg tttaaagagc
     3001 gtatccgtat tcagattcag gacccgatca tgcgaaaagc tgtggctaac gcacaggagc
     3061 gcatagggac taatcgacag aaaatggttg atgaactggg acactgggag gaatggcgtt
     3121 cacgagctga acagattcgt cggcatgttc tggaaaatct tgatgcttat ttgtatcaat
     3181 tgtccgaaaa ggttactgcg aatggcgggc atgtatattt tgcccgtacc agagaggatg
     3241 caaccagtta catactgaag gtggcgaaag aaaaaaacgc caggaaggtt gttaaatcca
     3301 aatcaatggt gactgaggaa attggcatga atgccgttct tcagaaagcc gggatacccg
     3361 tgattgaaac ggaccttggg gaatatattc ttcagctagc acatgagcca ccatcgcatg
     3421 tggttgtgcc ggcaatacat aaagatcgcc atcaaatccg cagagtttta catgaacatc
     3481 tggggtatga cgggcctgaa actccagaag caatgacatt gttcatccgt gagaaaatca
     3541 gacaagattt tctgagtgct gaagttggcg taacggggtg taactttgcc gttgcagaaa
     3601 cagggtcagt ctgccttgtt acaaacgaag gcaacgcacg gatgtgcacc acattaccta
     3661 aaacacatat tgctgttatg ggtatggagc gtattgctcc gacattccag gaggtggatg
     3721 ttctgattac catgcttgca aggagtgctg tcggggcgag actgacaggt tataacacct
     3781 ggcttacagg ccccagagaa gaaggacatg ttgatggtcc ggaagagttc catctcgtta
     3841 ttgttgacaa tgggcgttca gagattctcg gctcagaatt cagcgatgta ttgcgatgta
     3901 tccgctgcgg tgcctgcatt aatacctgcc cggcttatcg tcagattggt gggcatggtt
     3961 atggttcaat ctacccgggg ccaatcggcg cggttatttc cccattgctt ggtggatatg
     4021 aagactttaa ggatttaccc tatgcctgct cattgtgcac tgcctgcaat ggggtatgtc
     4081 ctgtaaaaat acctctggca acgctaatac aaaaacatcg ccaggttatg gcagagtctg
     4141 aaatcacacc attaacggaa cgccgtataa ccagagcatt caattacata aacagccatc
     4201 cggcgttgtg gaaggtaggt atggttgctg gggccaaaac agcccgtctg ttcatcagga
     4261 aaggaaaaac gccgattagt gttggagcga taaaagaatg gactgaagcc agagacctgc
     4321 catcacctga tggagaaagc ttccgtagtt ggttcaagcg acataagaag gaggggtaat
     4381 ttatggaaaa cagagaggaa tttctcgttg cgatcgccag aaagcttggc agaccagtaa
     4441 gacatattcc cgaagcaatg ccggagcctg taaatacgct ggctacgaca cgtttaacag
     4501 agttgacatc agaccaacgc tgtgaggcgt ttattaaatt tgcctcggaa gtgatgcttg
     4561 ccgagtgtgt tctggtgtcg cctgagaacg caccatcaac agccttagac atctgttgga
     4621 agttcggttc cgggccagtc attatcagca acgatcaacg actggtcgat acagggatta
     4681 cgccattgct acaaaaagaa atggacgctg ctctgtggga tccggagaag ggcgatgaaa
     4741 atatccatct tgctgaaaag gcaaaaactg gtgtcgtttt cgctgagtat gggctgacag
     4801 aatcaggagg cattgtctta ttctccgcac cagaacgtgg gcgctcagta agccttttgc
     4861 ctgaagcatc attatttgtt ttacgtaaaa gcaccattct ccccagagtg gcgcagcttg
     4921 cgcaaaaact tcatgatatg gctcaggaag gacaacggat gccgtcctgt ataaatctga
     4981 ttggaggccc aagttctact gcagatatag aactgatcaa agttgttggc gtacatggtc
     5041 ctgtacatgc aacctatttg atcattgatg attgcgatta acgattgcta ataaaaaaat
     5101 ggccttaagg tgatatttcg tcttgtgaaa aggcaacaat agactaaagg ttaggcataa
     5161 ttgtgtttct ttatgcctcc gcagcaattt gtggaggctt ttttgcttac tccaaacagt
     5221 ttggcttctt gaaaaagtca tatcccaaac ctattgacgc ttataaaaaa gtaaagaatg
     5281 gtgagtaagc gtaaactgac caccgtatgt agccatgatg ccagtattgg taacgtaggt
     5341 gcacccctat ctatttttgt agaaccggag taaacagacc agatagttta aatgagctgg
     5401 ttatgcgtaa cataatttat ctattttggc tcttacaaaa tagataaaga aggagtatct
     5461 gatgagatac tccattatga cagactcaac tcatgtgatt tttcttaaag ttctctgctt
     5521 gcgccagtac ccccatataa acatcatcat tcgctactgg cgggaactta tgcttgtgta
     5581 gaagcagaat aagttcaact ttcagtttcg ctttaatatc atcgcgttta ctccagtcag
     5641 gatatttcga tgtgttgtca accacgcttt tcatctcttt tgccagcgac agcatttttt
     5701 catcgtcata ggtgaactga tatttatcgc gcatatgagc aagaatgtcg aagaacgctt
     5761 tttcttcaat atcaatacct aaatcggccc aggtgcccat ttctgtttta atatcataga
     5821 taatatcggt catttcctga ctgaatgtat cgaattcttc accgttgagt acatcatctt
     5881 ctcgccgctc attataacga tctataatag cctggaagcg gcgggtgaag ttaatccctt
     5941 gcaactggtt cactttcctg aagtcgctga tcgctttttc cagtaatttt tgtaatagct
     6001 ggatcttcgt tgccggaagt ttgatcttgt taattcgcgc cagataatct tcgtcaaaaa
     6061 tatcgatgga ttccgctttt ttatcgccaa gaaaataaat ttcttctacg ccatcagctt
     6121 ttagcgcttc tgcaatcatt tcacgaacgc gtgcattcat ctgggtaaca tccggtgcgt
     6181 cacctttcgt cagtttgaaa acaattgaac gaacagcaag ataatagtga atatgatcac
     6241 gttctgtctg tgatagtgct tcactgccgc agcagacgtc gtaggcggct ttcatgcgtt
     6301 taaccagtcc cataaaacga cgttcaactt tctgggtacg cagaacgaat tccgccgcgc
     6361 ggttgaggca ggataattgc gcttgtggct caccactaaa ataatcccga ctgtcaaagt
     6421 cgtaaaagac ttgccccaac aaatcgagat ggtttttaac ttcagtcacc gattgctgaa
     6481 tatcttcaaa gttggtggca tcaatgcggg aatacattgc cagtgcctgg ttcatctgac
     6541 ttttaatgcc gatgtagtcc accactaacc ctttgctttt gccttccagt ttacggttaa
     6601 cgcgagaaat agtctggata aggttatgtt tttgtaaggg cttatcaata tagatggtat
     6661 ccagttcagg aacatcaaaa ccggtcagcc acatatcaac aacaatggca attttgaaat
     6721 tcgatttagc gtttttgaac tgcttatcca gctctttgcg atattctttt gtgcccagta
     6781 aatcataaag cgcctcgtcg tcatctttac cgcgcgtcat gaccatcttc accatttcag
     6841 aaggcggtaa ctctttttgc tcctgttctg tcaggaagac gccatcgggg gcttgcttca
     6901 cttcaaacca ggcagggcga atagctttaa gctggcggta gaaatcccag gcaatttcac
     6961 ggctggcaca aacaaacatg gctttgcctt ttacggtgga accttcggct acgcgttttt
     7021 cataatgttt ggcaaaatct tccgcgaggg cttttaatcg atcttcatca cccagaaccg
     7081 cattcatggt tgcggtggct tttttgcttt cgtcgatttg ccactcattg gtgcctgcgt
     7141 ttgcgcactc ttcgtaatac ttttcgactt cttccagttt gctggagtcc aggatcactt
     7201 tagccgcacg gccttcgtac acgatgcgta cagtgatttc atcctgaacg gattcggtca
     7261 tggtgtaact gtcgatcacc tcaccgaaga catcgagcgt cgcgtcaatc ggtgtgccgg
     7321 taaagccaac ataggtggcg tttggcagtg aatcgtgcag gtatttcgca aagccatacg
     7381 ttttgcgcac ttttccgctt tctttatcga cgatgacttt ctggtcgagg ttaacctggc
     7441 tgcgatgtgc ttcgtccgag atgcaaatga tattgctgcg ttccgaaagg agttcggtgt
     7501 cttcggtgaa tttatggatc gttgtcagga atacaccgcc actattgcgt cccgccagtt
     7561 gattacgcaa atcttcacgg ctggtaacgg gaaggatggt gtcgtcacca atgtaatttt
     7621 tggcgttgca catttgcgca gaaagctgat cgtccagatc ggtgcggtcg gtgatcaaaa
     7681 caatggtcgg gctggcaaac tctacgctct tcatcaaaag acgcgttaaa aattgcatgg
     7741 tgtaactttt gccacagccc gttgcgccaa agtaagtgcc gcctttaccg ttaccgaaag
     7801 gtttacgcgc ttgcttgatg ctgtaataga gtttgcgggc ggcatagtac tgcggatatc
     7861 ggcagcaaat ttttacttcg tgcctggctt tatccgggaa gcagataaag tttttaatta
     7921 catccagcag acgtaccgga tgaaacagcc cctgaatcat tgagtgcaat gatggaattc
     7981 catcctgttc acggttttca ttaccggtga cttttcgcca tgagtaaaaa tattcatagg
     8041 gcgcaaacag gttgcccatc cggttattaa ctccatcgct aataatgcag agcgcgttgt
     8101 agataaacag ttgcggaata tcccggcgat agcgtttgca gagttgtctc caggcattgc
     8161 caatactagc atcctgctcg cgcaccgcac ttttaaattc aaagacgacc agtggcagac
     8221 cgttgatata gagaatgccg tcaggaatac gactctgatt atctttgccg gagattttaa
     8281 gctgattaac aatcttaatc aggttaagcg gcggattaat ataggagggc gggagttccg
     8341 cagcctgttg caacaggaca tcttcggcgt caaatatctg gcgcagtgcg gcaggtagat
     8401 gccgggtgtc gagcaattca atgtagagat ctttttgttg ccgatcgtcg cgtttgaaca
     8461 gaaaaccatt tgccagccag cgacaaaatg ttttgttgct ttcataaaga tcggaagccg
     8521 gaagcgtggt gaactgtttg atcagtcgct gaatctcttc ttcagtaatg ccatcaggct
     8581 ggtagcgtgc cgctaaataa tgacggagat cgtcttcgat aatgacctga tcgagactcg
     8641 aacgtgggac attatcgccg atcagatgtt gatacccctg atcctgtaac agttcaatga
     8701 tcgcttgttc taattttgct tcactgaaag agagactcat atcgcgctcc aaccctggct
     8761 aatttctggt aagcattcct gtatcagtag aaaaccttac cagaaagccc ctgattttac
     8821 aggggcaaaa tgcgttatgc ggcttctgta tcggtgctga gatccggaag atcttccagg
     8881 gatagctcac cggagataag tttcgggagc agggtgttgc ggagggctgt tagtgagact
     8941 ccacattttc tatttttact aaagcgatgg aataaagggg tgctaaattt ttcaaactct
     9001 ttaagcaaaa ccattggggg atttaggaat gatatatttt tcaaatgtgt tgaaggctga
     9061 gcaattgaag gaacaccaac ctgagaggcg ttagaaagca gagcatgttg tcctttttct
     9121 gacctaaaat aatgaatcaa atagtacggc gatgattttg attcatttac gcgtaagaaa
     9181 aattgacgtt gggaaattat atatctgtca tattcagaat cttcaggaat taaagaaact
     9241 tggccaatat tacctgcatg tgtaaaaatt atgtcttttc tatatacagc agagtttttg
     9301 agcttttcag catgctctgg agtaatgaaa ttattatccc catcgataag aaggagggct
     9361 ttcagatgtt ggccgcttat aattggtaca ccagcattaa caaatgtgga tactttaata
     9421 ttggaaccaa atggtcccat tcctattttc aatgcaatat cttcagttcg aacaatactc
     9481 catcctttcg gcacccaacc caactccgtt tcttcaaatt cacttgggaa aagcgaacgg
     9541 atttccgccg gaagcggttt aaaatctgta ctattacgta ctttttgacg taattcggca
     9601 cgagattgca gagcttccgg gattggattt cctgcatcca gagcgttata aatcaccgga
     9661 tcaaaatcca caaaccagga tttgaagaga gtttgggaca tttgttctaa tgacttattt
     9721 attact
//
LOCUS       DAZFQD010000172         9668 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.172, whole genome shotgun sequence.
ACCESSION   DAZFQD010000172 DAZFQD010000000
VERSION     DAZFQD010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9668
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.172"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            210..1523
                     /gene="gltP"
                     /locus_tag="ACHKTW_004547"
     CDS             210..1523
                     /gene="gltP"
                     /locus_tag="ACHKTW_004547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006835 - dicarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HFO6465451.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVPGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(1601..2290)
                     /gene="yjcO"
                     /locus_tag="ACHKTW_004548"
     CDS             complement(1601..2290)
                     /gene="yjcO"
                     /locus_tag="ACHKTW_004548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YjcO"
                     /protein_id="HFO6465452.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(2384..4531)
                     /gene="fdhF"
                     /locus_tag="ACHKTW_004549"
     CDS             complement(2384..4531)
                     /gene="fdhF"
                     /locus_tag="ACHKTW_004549"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(4112..4114),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HFO6465453.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVSWDEALNYVAERLSAIKEKYGPDA
                     IQTTGSSRGTGNETNYVMQKFARAVIGTNNVDCCARVUHGPSVAGLHQSVGNGAMSNA
                     INEIDNTDLVFVFGYNPADSHPIVANHVINAKRNGAKIIVCDPRKIETARIADMHIAL
                     KNGSNIALLNAMGHVIIEENLYDKAFVASRTEGFEEYRKIVEGYTPESVEDITGVSAS
                     EIRQAARMYAQAKSAAILWGMGVTQFYQGVETVRSLTSLAMLTGNLGKPHAGVNPVRG
                     QNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVESLPAHTGYRISELPHRAAH
                     GEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFMTKTASAADVILPSTSWGE
                     HEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMGYPMHYNNTQEIWDELRHL
                     CPDFYGATYEKMGELGFIQWPCRDTSDADQGTSYLFKEKFDTPNGLAQFFTCDWVAPI
                     DKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPGYAQINTEDAKRLGIEDEA
                     LVWVHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGACNELVTENLSPITKTPEYKYCA
                     VRVEPIADQRAAEQYVIDEYNKLKTRLREAALA"
     gene            complement(4729..6195)
                     /gene="mdtP"
                     /locus_tag="ACHKTW_004550"
     CDS             complement(4729..6195)
                     /gene="mdtP"
                     /locus_tag="ACHKTW_004550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HFO6465454.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDAIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAERVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQAAPVVEKK"
     gene            complement(6192..8243)
                     /gene="mdtO"
                     /locus_tag="ACHKTW_004551"
     CDS             complement(6192..8243)
                     /gene="mdtO"
                     /locus_tag="ACHKTW_004551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HFO6465455.1"
                     /translation="MSALNSLPLPVVRLLAFFHEELSERRPGRVPQIVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYGEP
                     LIRLIIAGPILMGCMFLMRTHRLGLVFFAVAIVAIYGQTFPAMLDYPEVVVRLTLWCI
                     VVGLYPTLLMTLIGVLWFPSRAITQMHQALNDRLDDAISHLTDSLAPLPETRIEREAL
                     ALQKLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKL
                     ASEINKLQHAVAKGQCWQSDWRITESEAMAARECNLENICQTLLQLGQMDPNTPPTPA
                     AKPPSMVADAFTNPDYMRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVANPNVG
                     SSYQKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERS
                     SYIGTQMVVTFALATLENVFGPVYDLVEIRDRAMGIIIGTVVSAVIYTFVWPESEART
                     LPQKLAGTLGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLD
                     SEERALLIERSQTVIRQGRDILHAWDATWNSAQALDNALQPDRAAQFADALEKYAAGL
                     ATALSRSPQITLEETPASQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGAT
                     Q"
     gene            complement(8243..9274)
                     /gene="mdtN"
                     /locus_tag="ACHKTW_004552"
     CDS             complement(8243..9274)
                     /gene="mdtN"
                     /locus_tag="ACHKTW_004552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HFO6465456.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVGSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPFEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSINATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMATVRAPFDGR
                     VISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            complement(9293..9568)
                     /locus_tag="ACHKTW_004553"
     CDS             complement(9293..9568)
                     /locus_tag="ACHKTW_004553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtcA family lipoprotein"
                     /protein_id="HFO6465457.1"
                     /translation="MPTVLSRMAMQLKKTAWIIPVFMVSGCSLSPAIPVIGAYYPSWF
                     FCAIASLILMLITRRVIQRANINLAFVGIIYTALFALYAMLFWLAFF"
BASE COUNT         2176 a         2578 c         2596 g         2318 t
ORIGIN      
        1 attaatcctg ccataaactc gcgttattat gcattaatgc agcgaaaagc tctgttgtta
       61 aagggttgcg caacataccg cgcaaatgat actgatcata agcgttaaaa aaatctacaa
      121 accaacgcaa cacaattcat gccctggcag tatgtcacgt tctcgcgttt ctgaacgggg
      181 aacggcgctc cattgaggaa gttattctga tgaaaaatat aaaattcagc ctggcctggc
      241 agattctgtt tgctatggtg ctgggcattc tcctgggaag ctacctgcac taccatagcg
      301 acagccgcga ctggctggtc gtcaatttgc tctctccggc gggtgatatc ttcatccatc
      361 tgattaaaat gattgttgtg ccgattgtga tctccacgct ggtggtgggt atcgcgggtg
      421 tgggtgatgc caaacagctc gggcgtattg gcgcgaaaac cattatttac ttcgaggtga
      481 tcaccaccgt cgccatcatt ttggggatca ctctggcgaa cgtcttccag cccggtgccg
      541 gggtcgatat gtcgcagctg gcgaccgtcg atatctcgaa atatcagagc actacggaag
      601 cggtacaaag cagttcccac ggcattatgg gcacgatttt gtcgctggtg ccgacgaaca
      661 ttgtggcgtc gatggcgaaa ggcgaaatgc tgccgatcat cttcttctcg gtgctgtttg
      721 gtctggggct ttcctccctg cccgcgacgc atcgtgaacc gctggtgacc gtgttccgct
      781 ccatctctga aaccatgttt aaagtgactc acatggtgat gcgttatgca ccggtgggtg
      841 tgtttgcgct gattgcggtg acggtggcta actttggttt ctcttctctg tggccactgg
      901 cgaaactggt gctgctggtg catttcgcca ttctgttctt cgcgctggta gtgctgggaa
      961 ttgtggcgcg cctgtgcggg ttaagcgtct ggatcctgat tcgtattctg aaagatgagc
     1021 tgattctggc gtactccact gccagctctg aaagcgtact gccgcgaatt attgagaaga
     1081 tggaagccta cggagcaccg gcgtcgatca ccagcttcgt ggtgccgacc ggttactctt
     1141 ttaaccttga tggttcgacg ctgtatcaaa gtattgccgc tattttcatc gctcagctgt
     1201 atggcattga cctgtccatc tggcaggaaa tcattctggt gctgacgctg atggtgacct
     1261 cgaaagggat tgctggcgtg ccgggcgtgt cgtttgtggt gctgctggca acgctgggta
     1321 gcgtaggtat tccgctggaa ggcctggcgt ttattgctgg tgttgaccgt atcctcgaca
     1381 tggcgcgtac tgcgctgaac gtggtgggta atgcgctggc ggtgctggtg attgccaagt
     1441 gggaacacaa atttgaccgc aaaaaggcgc tggcttatga gcgtgaagtg ctgggcaaat
     1501 ttgataaaac tgcggatcaa taattgaaga ttgccggggt tatccacccg gcaatgtgtg
     1561 gatgcctgat gcgacgctgg tgcgtcttat caggcctacc ttaaccgttg gttaattttt
     1621 caaactcttc acacccggta tcaaaccctt ccatacagct caggttcaac cagtgcagcg
     1681 ccttttgttt gttcttctcg ataaaacctt cctcaccgtt taagaacatc attcccgccc
     1741 agtactcgga ataaccggtt cgggaaattg cagagctgcg tttgaaatac caggttgcct
     1801 tgtcatcgtc ggccttaatg cccacgccgt tggcgtaaat caaaccaagc agcatttggg
     1861 catcgacggc ggagtcattc tccagatctt ccgaggcgtt ttctaacagc gaaatcgcct
     1921 ttggataatc cggtttaccc gcttgagtat ttaccagaat atgcgccagc gttacttcac
     1981 cttctttact gcccgcttgc gccgctttct ctgcaagaac ttttgcttgt ggatagtcca
     2041 gactgaccgg attggtgatt ttgatctgcg ccagcagcgc gcaggcatca gcatcaccgc
     2101 tgtcggcggc tttctgtgcc caatattcgg ctttgctcaa atcgccggag ctaaaccagc
     2161 tgtcggcaag aaaatattgt gcgcgtcggt ccccggcctc tgctgccttt aaatactggc
     2221 tgccaggctc ggagtcgttc gcgtgggcaa agaatgtcaa aaacaacatc aatgcgataa
     2281 tttttttcat tttgataata cgattgggta gggagtaacc agtataaaga gttcgaatgg
     2341 ataaaaaaac agcctccgaa aggaggctgt agaaaggact atattacgcc agtgccgctt
     2401 cgcgcaggcg agttttcaac ttgttgtact cgtcaatcac gtactgctcg gcggcgcgct
     2461 gatcggcgat cggctcgacg cgcacggcgc agtatttgta ttccggcgtt ttcgtaatcg
     2521 ggcttaagtt ttcggtaacc agctcgttac aggcaccaat ccaccactgg taggtcatgt
     2581 aaatcgcccc tttgttcgga cgatcgctga cctgcgcacg ggtgataatt ttgcctttac
     2641 gcgagtgcac ccaaaccaat gcctcatctt caatacccag acgtttggcg tcttcggtat
     2701 tgatttgtgc gtagccaggt tcatcagcca gcgccgccag tgccgcacag ttaccggtca
     2761 tcgaacggca agagtagtga ccaacttcac gcaccgttga cagtaccatc gggtactcgt
     2821 cggtgagttt gtcgattggc gctacccagt cgcaggtgaa gaactgtgcc agaccgttcg
     2881 gggtatcaaa cttctcttta aacagataag aagtcccctg atcggcatct gaagtatcgc
     2941 ggcaaggcca ctgaatgaag cccagttcgc ccattttctc gtaagtcgca ccgtagaaat
     3001 ccgggcacag atgacgcaac tcatcccaga tctcctgggt gttgttgtag tgcatcggat
     3061 aacccatacg ggtggcgatt tcactgatga tttgccagtc cgttttcaga tcccatttcg
     3121 gttcaactgc tttgaagaaa cgctggaagc cacggtcagc cgcagtaaac acgccttcat
     3181 gctcgcccca cgacgttgac ggcaaaatga catccgccgc cgacgcggtt ttggtcataa
     3241 agatgtcctg aacgataacc agttccagat cttcaaaggc tttacgtact gccgacagct
     3301 ccgcgtcagt ttgcagcgga tcttcgccca taatgtacgc ggcacgcact tcgccatgcg
     3361 ctgcgcggtg cggcagctcg ctgatgcgat aaccggtatg agcaggcagg ctttccacgc
     3421 cccaggcttt ggcgaatttc tcgcggttag ccggatcttt cacgtactgg tatcccggat
     3481 acgtatccgg cagcgcgccc atatcgcagg caccctgcac gttgttctga ccacgaaccg
     3541 ggtttacacc cgcatgcggc ttaccgaggt taccggtcag catcgcgagg ctggtcagag
     3601 aacgcacggt ttccacgccc tggtagaact gggttacacc catgccccac aggatggcgg
     3661 cgcttttcgc ctgggcatac atccgtgccg cctgacgaat ctcactggcg ctgacgccgg
     3721 tgatatcttc aaccgactcc ggcgtgtagc cttcaacgat tttacgatac tcttcaaagc
     3781 cttctgtacg ggaagcgacg aacgctttgt cgtacagatt ttcttcaata atgacatggc
     3841 ccatcgcatt caacagcgcg atgttcgagc cgtttttcag tgcaatgtgc atgtcagcaa
     3901 tgcgcgcggt ttcaattttg cgcggatcgc agacgataat tttcgccccg ttacgtttag
     3961 cgttaattac gtgattcgcc acgattgggt gggaatccgc cgggttgtac ccgaaaacga
     4021 acactaaatc ggtattatca atttcgttaa tagcattgct cattgcgcca ttaccgaccg
     4081 attggtgcag acctgcaacc gatgggccgt gtcagacacg agcgcagcag tcaacgttat
     4141 tggtaccaat aacggcgcgc gcaaattttt gcattacata gttggtttcg ttacccgtac
     4201 cacgcgagga gccggtcgtc tggatggcat ccggaccgta cttctctttg atggcgctca
     4261 ggcgctcggc aacgtaattc agtgcctcat cccaggaaac aggttcgagt ttgccgccac
     4321 gctggcgacg gatcatgggg gttttcaggc gcggggtcag gatctgggta tcgttaatga
     4381 aatcccagcc ataataaccc ttcagacaca gggtaccctg gttggttttc ccctgcgctg
     4441 cctccgcccg gacgattttg ccgttatcga cgaccaggtt gattttgcaa cctgatgcgc
     4501 aataggggca aaccgtgacg acttttttca tcggtctcgc tccagttaat caaatcacgc
     4561 atacgcgctc tcgactacag tatgcatctt ttatgccaca ttttatttgg ggtcattccc
     4621 tgatattacg ggcactattt attcaaaact ctgacgaaaa acaggctgtc gtcagttttg
     4681 acgtgacgaa acgaaatacc gcgtgacagc catcacgcgg cagacatttt attttttctc
     4741 gacgacggga gccgcctgat acccgccgcc cagcgatttc atcaactgaa tgctttggat
     4801 cacccggcgg ctgtccagca tcagtaatga catctcttcg gcaagtactg gcaaccgggc
     4861 ttcagtggcc tgtaagcggc tggttaagcc gcgctgatag gcggcctcgg cagcgcgctg
     4921 ggtgaagcgc gttgcttcca cgcgttcagc ctgcatttct cgctcgtcgt tgagcgtttg
     4981 cagacgcgtg ccgttgacgg caacgtcacg caccgcgttc agtactgact ggttgtaacg
     5041 ttcaatcatc atgttgctgg cggcgcgcgt gccttcgaga ttggcattca accgtccacc
     5101 gtcaaacagc ggcaatttca gacccgggat gaagttgaac tggcgactgg tttttttgaa
     5161 taaggtatcc aggtggatgg catcaaggcc gaaaaacgct ttgatatcaa agctcgggta
     5221 gaacagcgcc cgcgcggaat ccacctgatc taatgacgcc tgaacatacc agcgcatggc
     5281 ttgcagatcc gggcgtctgg cgagcaactc ataagagagt gtcgccggaa tgccggtctg
     5341 gactcgtggt aatgccaccg gtttgatctc cggcatatcg ctggcccccg cgccaatcaa
     5401 tgcacgcaga gattctcgcg tttcggtgat ttgccctttg acggcagcaa tttgtttatc
     5461 gaccgccaga atctgtgccc gcgcgccgtg gaaaggcact tgtgcttcca gaccgtgcgc
     5521 cactttactc tggtgcgctt tcaccgcgta atcaatcaca tcgcgagttt gttctaacag
     5581 atcgagcatc tgatagctgg cctgcatact gtaataaagc tgcgctacgc ccgtggtcag
     5641 cgatagctct actgctgcgg tttctgccag cgcggcatta tgcgcgccaa tggcggcggc
     5701 aaccgctgag cgatgcacac cccacaaatc aagatccagt ccggcaaaca aacctactgt
     5761 ggcttccgta tagtacggcc cgtccatacc cagtgcgggc gcatccatcg cataagggct
     5821 taaaaagccg ttcgccgaga cgcgttggcg gttgagcatc cctaacgctg cgacctgtaa
     5881 ttgtgaaccg gcatctaaca aatcggcctg ggactgcgct ttttcttccc gcagtttcgc
     5941 ttcggcgagg gtgtgtgaac cacttagcgt ccgttggatc agcgcatcca gctgcgggtc
     6001 attgagttgt ttccaccact gcgcctgcgg ccagccggag ctggcaagat gaatatcgtc
     6061 ggccagtttg atttgttccg gtttgagctg ttgatgaggt gcagaatcct tacgtaccag
     6121 ggcacagccg gaaatcagcg tcgtgctgcc gagaatgctg cacaacagca gacgtgaaag
     6181 ttgacgattg atcattgcgt ggctccttgt gcctgttccg tggcgggcgt taatgccggg
     6241 gctgtccagt ctggcaagcg ggcgaaaagc tggcagacgt gttgctcctg ttttaataag
     6301 gtgggcagga tggcctgcga ggcgggtgtc tcttcaagcg ttatttgagg agaacggctg
     6361 agtgcggttg ccagaccggc agcgtatttc tccagggcgt cggcaaactg agctgctctg
     6421 tccggctgta gtgcgttatc cagcgcctgc gccgagttcc aggtggcatc ccaggcgtga
     6481 agaatatcgc ggccctgacg aataaccgtt tgcgaacgtt caatcagtaa ggcgcgttct
     6541 tcgctgtcca gttgacgctc cagcgccacg cgttggcaca tctcttcaca ggcattaaac
     6601 gccgcatgca gaccgatacg aatttgcaga taagtgcgca gagccgtgac ttcctgctgg
     6661 cgtgggatcc gcattacttt acttaacata cccagcgtgc cagccagttt ttgcggcagt
     6721 gtgcgcgctt cactttcagg ccagacaaag gtgtaaatca ccgcggacac cacggtacca
     6781 atgatgatac ccatggcgcg atcgcgaatt tccaccaggt cgtacaccgg gccaaaaacg
     6841 ttttcgagcg tggcgagcgc gaaggtgacc accatctgtg tgccgatata agaagagcgt
     6901 tcagagctgg tggcaatcca tgcgcccaac aggaaaatcg gtgccagcac aaacagcaat
     6961 tcgacaatat tgtccagcca gggcatgacc agtagcgtga ataacagcgc cagaatcgcg
     7021 ccgcaaaagg ccccgccaaa acgcagcacc atcttctggt acgacgaacc gacatttggg
     7081 ttagcgacga tcacgcatgt cagcatacag gtgtgaatgc cttcccagtc cacgccgctg
     7141 tagaaggtgt aacagatcaa acaggcgagc agtgttttta ccgcgtagcg catatagtct
     7201 ggattggtaa aagcatcggc gaccattgat ggcggtttgg cggcgggcgt tggcggcgta
     7261 ttcgggtcca tctggcccag ttgtaacaac gtctggcaga tattctccag gttacattcc
     7321 cgtgccgcca tcgcttcact ttcagtgatc cgccagtcgc tttgccagca ctgaccttta
     7381 gcaacggcat gctgcagctt attgatttct gaggctaatt tttgccgaaa ttcaataatt
     7441 gcctgagaat cagcaaaaga ggtgggatcg tagcgattca gcgtcgagta aatgtaggtt
     7501 accgttgcca cgcagctttg ccaccatgcg ctttgagttc gccagttggc atcgtccgcg
     7561 agacaaaaga cattcagttt ttgcagcgcc agcgcctctc tttcaatccg cgtttcgggt
     7621 agcggtgcga ggctgtccgt caggtgacta atggcatcat caagccgatc attaagcgct
     7681 tgatgcattt gcgtaatggc acgactggga aaccacagca cgccgattaa cgtcatcagc
     7741 aaggttggat agaggccaac aacgatacac cacagcgtta agcgcacgac cacttccgga
     7801 tagtcgagca tggcggggaa ggtttgcccg taaatggcga caatggcgac agcgaaaaag
     7861 accagtccca gccgatgggt gcgcatcaaa aacatacagc ccatcaggat cggcccggcg
     7921 atgatcaatc ggatcaacgg ttcgccgtat gaccatttgt agatcaaaaa caggctgccg
     7981 atctccagca ccgtggcaac cacaaacaag atcgcgacaa atttggtgta aaacgcgttc
     8041 gactgaatac cgtaaaacag cactgccagc gataacgcca caaaagggat ctcaaaagtc
     8101 atcgagatca gaatcaccag caggcagcct acccagagtt gcacgatctg cggcacgcgg
     8161 ccgggtcgcc gctcgcttaa ctcttcatga aagaacgcca gcagcctgac caccggtaat
     8221 ggcagggagt tgagcgcgct cattattgcg gctcaagatt agcgactgcc gaagcgccga
     8281 tgcggaacat ttccgggtca ggtttatcga ccatgatttt gaccggaaaa cgctgggcaa
     8341 cgcggaccca gttaatagag cgagaaactt tcggcaggcc gcccagcacc aggccgccgt
     8401 catccggtag cacgccgtag ccaatcgaat ccactttacc ctcgaaggtt ttgccgctgt
     8461 cactcatcag gcgaatcgtt gcgggtgtac ctgagcgaat atttttcaga tcggtttcgc
     8521 ggaagttggc gatcacgtac cagtgacgag tgtcgattag ggtaaaaata gggcgcatgg
     8581 cagaagcaaa ttgcccgacg gaggttttga gggaaatgac ccggccatca aacggcgcgc
     8641 gaacggtcgc catttccaga tgaagtttgg tcagggcaat atccgcttcg accgccgcac
     8701 gttgcgccac cagcgcatcg acgccgctga cggcgctggc ggctgactgc gcctgtaaca
     8761 atacggcatt aagatcggct tctgcggcgc gctgcgcagt tcttgcacgg tcaacgtctt
     8821 ccgctgagac aaaaccctct ttcagtaatg gctcggtgcg gcgtaatgta tctgtggcct
     8881 gtttcgcggc ggcacgggct ttttctaccg tggcattaat cgagtcggca ccaaactgtt
     8941 gcgcatcaac gctacgctgg gtgagcataa tttgcttatc cagcgccgcg agggaggctt
     9001 cagctttcgc cagattggct tcgaacgggc gcgggtcaat gcggaacagc aaatcgccct
     9061 gtttgactgc ctggttgtcg gtgaccgcca gttctacaat gcggccgctg acttccggca
     9121 ccacatcaat ggtatctgct gacgcgtaag cgtcattagt tgatggcgca ctgcctacgc
     9181 gccagataac gaaaacaagg gcaaccagcg ccaatgcaac cactaacaga gcagggaatt
     9241 tactgcgagg agcttttttc ggcgtacttt ccatgagcgc gtctcaaata gattagaaaa
     9301 atgccagcca gaacagcatg gcgtagagag caaaaagggc ggtataaata attccgacaa
     9361 atgccagatt gatatttgcc cgctgaataa ctcgcctcgt gatgagcatc aaaataaggc
     9421 tggcaatggc gcagaaaaac cagctgggat aataagcacc gatcaccggg attgccggag
     9481 ataatgagca tcccgaaacc atgaagacgg gaattatcca ggctgttttt ttgagttgca
     9541 tcgccatgcg agagagaact gttggcatat taaatacatc ctgtctgctg cgaacaatat
     9601 ctgtagtgaa tgtcaatatc cattgaaggc gtcagtatgg ctattttctc gatgaataaa
     9661 aatagctt
//
LOCUS       DAZFQD010000173         9640 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.173, whole genome shotgun sequence.
ACCESSION   DAZFQD010000173 DAZFQD010000000
VERSION     DAZFQD010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9640
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.173"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(60..272)
                     /gene="cspA"
                     /locus_tag="ACHKTW_004554"
     CDS             complement(60..272)
                     /gene="cspA"
                     /locus_tag="ACHKTW_004554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HFO6465458.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(553..843)
                     /locus_tag="ACHKTW_004555"
     CDS             complement(553..843)
                     /locus_tag="ACHKTW_004555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
                     /protein_id="HFO6465459.1"
                     /translation="MEYKDPMHELLSSLEQIVFKDETQKITLTHRTTSCTEIEQLRKG
                     TGLKIDDFARVLGVSVAMVKEWESRRVKPSSAELKLMRLIQANPALSKQLME"
     gene            1277..1987
                     /locus_tag="ACHKTW_004556"
     CDS             1277..1987
                     /locus_tag="ACHKTW_004556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HFO6465460.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQSDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(2037..3011)
                     /gene="ghrB"
                     /locus_tag="ACHKTW_004557"
     CDS             complement(2037..3011)
                     /gene="ghrB"
                     /locus_tag="ACHKTW_004557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HFO6465461.1"
                     /translation="MKPSVILYKALPDDLLQRLQEHFTVHQVANLSPQTVEQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRAHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(3115..3774)
                     /locus_tag="ACHKTW_004558"
     CDS             complement(3115..3774)
                     /locus_tag="ACHKTW_004558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HFO6465462.1"
                     /translation="MKKRVYLIAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSL
                     VGAGIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRS
                     GDNIILNMPNNVTFDSSSATLKPAGANTLTGVAMVLKEYPKTAVNVIGYTDSTGGHDL
                     NMRLSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSP
                     L"
     gene            3927..6260
                     /gene="bisC"
                     /locus_tag="ACHKTW_004559"
     CDS             3927..6260
                     /gene="bisC"
                     /locus_tag="ACHKTW_004559"
                     /EC_number="1.8.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin sulfoxide reductase"
                     /protein_id="HFO6465463.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALDLIHQQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLARCTTGYA
                     VFASYLLGESDGIAKTAEWAAEICGVGAAKIRELAAIFHQNTTMLMAGWGMQRQQFGE
                     QKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGNPTRRSAVLSSMQGSLPGGCDAVDK
                     IPVARIVEALENPGGAYQHNGMDRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPEL
                     VVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRYEARNDF
                     DVFAELSERWEKGGYARFTEGKSQLQWLETFYNVARQRGASQQVELPPFAEFWQANQL
                     IEMPENPDSERFIRFADFCRDPLAHPLKTASGKIEIFSQRIADYGYPDCPGHPMWLEP
                     DEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQERGIQD
                     GDTVRLWNARGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDL
                     PSSRLGNGCAGNTALAWLEKYNGPELTLTAFEPPASS"
     gene            complement(6229..6669)
                     /locus_tag="ACHKTW_004560"
     CDS             complement(6229..6669)
                     /locus_tag="ACHKTW_004560"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFO6465464.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRHPHLML
                     EVYQKNQPAINFYQAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(6666..7229)
                     /gene="tag"
                     /locus_tag="ACHKTW_004561"
     CDS             complement(6666..7229)
                     /gene="tag"
                     /locus_tag="ACHKTW_004561"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFO6465465.1"
                     /translation="MERCGWVSQDPLYIAYHDNEWGVPETDSKKLFEMICLEGQQAGL
                     SWITVLKKRENYRACFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFVDFVWSFVNHQPQVTQATTLSEIPTSTSASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYPGNKP"
     gene            7387..8085
                     /locus_tag="ACHKTW_004562"
     CDS             7387..8085
                     /locus_tag="ACHKTW_004562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HFO6465466.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLTDPLWHASVSSLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALSQAENTTN
                     NSDYLYTMGVSARF"
     gene            8314..9516
                     /locus_tag="ACHKTW_004563"
     CDS             8314..9516
                     /locus_tag="ACHKTW_004563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO6465467.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTMASGILLGLGFFLTAHSNNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSICGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KVLREAHI"
BASE COUNT         2277 a         2357 c         2503 g         2503 t
ORIGIN      
        1 aaataaaaaa atccccgcca aatggcaggg atcttagatt ctgtgctttt aagcagagat
       61 tacaggctgg ttacgttacc agctgccggg cctttagcgc cgctttcgat ggtgaaggac
      121 actttctgac cttcgtccag agatttgtaa ccatcgttct ggatagcaga gaagtgtacg
      181 aacacgtctt tagagccatc gtcaggagtg atgaagccga agcctttgtc agcgttgaac
      241 cattttacga taccagtcat tttaccggac atagtgtatt acctttaata attaagtgtg
      301 cctttcggcg atatggcgtg ctttacagat tttgaagcgt taaaggaatg tgcactacga
      361 ggggtatcaa cgataactct tgaagggact tgccttacta cactgcttta atggtctgta
      421 cgtcaaaccg ttgatgtgca ttaagccacg cattggcggg tgatgcaaca attatttttc
      481 atatttatga ttaatcggtt ggaaaacggt cctgtcatca ggaccgtaaa cagcaataaa
      541 gtggataaaa gtctattcca tcaactgctt acttaatgcc gggttggctt gaatcaaacg
      601 catcaatttt agttcggcac ttgaaggctt cacgcgtctg gattcccatt cctttaccat
      661 ggcgactgat acgcccaaaa cccgggcgaa atcatcgatt tttaatcctg tcccttttcg
      721 taactgctca atttcggtac aggacgttgt tctgtgcgtc agggtaattt tctgcgtttc
      781 atctttaaaa acaatctgtt ccaggctgct caacagctca tgcattggat ctttatattc
      841 cattgaaaac tcctcaaatc acactgcggg atcgtgaatt gcatcgaagc tcattaagaa
      901 tagtcgggaa aagcagacac agcggggtga agtcagggca tgctctggct atgattcgat
      961 tcagccagtc atttagcgtg acttatagcc acctaacgat ctgataaatg ccgatatatc
     1021 tcttaccact cctggtttgt aaagatttcg aaaatctaac cttctgcgaa ggcgtaaatc
     1081 tctggctaca aaaaaagcaa cggcactatc attttttaac aatgaaacaa caaattaacc
     1141 ttgtttcatt ttctccatga ttttaccagg aagattacgt agaaagggta tcctgcaagc
     1201 ctgtcctgga ctatccttgt cacgtcagac acgcgtgtgt cgttgtgcgc tttttttggg
     1261 tgaaaggagt aagaaaatgg cgacaggaaa gtcctgctct cgctggtttg cgcctcttgc
     1321 ggcgttatta atggtagtta gcctgagtgg gtgttttgat aaagaaggcg atcagcgtaa
     1381 agcgtttatc gacttcctgc agaatacagt gatgcgtagc ggtgaacgtc taccaaccct
     1441 gactgccgat cagaaaaaac agtttggtcc ttttgtctct gattacgcga ttctgtatgg
     1501 ttattctcag caggtgaatc aggcgatgga ttccggtctg cgcccggtcg tagacagcgt
     1561 taacgcaatt cgcgtaccgc aggattacgt tacgcaaagc ggtccgctgc gtgaaatgaa
     1621 cggttcgcta ggtgtgctgg cgcaacaact gcaaaatgcg aagttgcagg ccgatgctgc
     1681 gcactctgcg ctaaaacaga gcgatgacct gaaaccggtc tttgatcagg cattcaccaa
     1741 agtggtaacg acgccagctg atgcattgca accgttgatt ccggcggcac aaactttcac
     1801 gcaacaactg gtgatggttg gagactacat tgctcagcaa ggtactcagg tgagctttgt
     1861 cgcaaatggc atccagttcc cgacttcaca gcaggcaagt gaatataaca aactgattgc
     1921 gccattaccg gcacagcatc aggcgtttaa tcaggcctgg actacagcag ttactgcaac
     1981 ccaataaaga gtaaaagccc gagcgattat cgggctttac tctacgcagt cgcggcttag
     2041 tccgcgacgt gcggattcac acagttcttc tcaacctttc cttgtaacgc atcaatcaaa
     2101 ttatccacgg cacaggcggc catgccataa cgcgtctcat gggtggcaga tccaatatgc
     2161 ggtactgcga cgacgttggc cattgagagc aacggcgaat ctacggacag tggctcttgt
     2221 tcgaagacat ccagcccggc ggcgtgaatt tcccctttct gcaatgctgc gatcagtgca
     2281 ttttcgtcaa ccaccgggcc acgtccggca ttaatgaaaa tggcggagga tttcattttg
     2341 gcgaattgtt ctgcgccaaa cagatgatgc gtctcatcag ttaacggcag gatcaggcaa
     2401 acgaaatctg actcttgtaa cagtgtatcc aaatcgcagt agcgggcgtt gaagcgttct
     2461 tctgcttctt tatggtggcg gcgcgcgtta tagaggatgg gcatgttgaa gccaaagtgc
     2521 gcacgttgtg ccagcgccat accgatccgt cccatcccga caatgcccag tgttttatgg
     2581 tgaacgtcag tgccgtacca gtccgggcct atgctcgcgg tccattcgcc tgcttttacc
     2641 cgttctgcta cctccacaac ccgacgagcg gtagacaaca ccagcgccat cagcgtatcg
     2701 gcgacggttt ctgttaatac ggttggcgtg tgcatcagca gaatttttcg ggcggtaagc
     2761 gcatcgacat caaaattgtc atagccgacg gagatcgttg atgtggcacg cagtttcggc
     2821 attttttcca gcaatgcggc atcaacattc tcgtttgaac ccagtaaacc ttcagcttcg
     2881 gcaaaaattg ctgcattttg ttcgacggtt tgtgggctga ggtttgccac ctggtgaacg
     2941 gtgaaatgct cttgcaggcg ttgcagtaaa tcatcaggta aggctttgta gaggataacg
     3001 gacggcttca tgcttctctc cattcactga taaggcgatc accttagcct gaaatatgat
     3061 gaaggaaaac tgaagaatgc caggtgattt ctgcatcacc tggcatgaaa gggattacag
     3121 cgggcttaag gtaatttcta cacggcggtt ttgcgcctta ccttctgcgg tgctgttgct
     3181 ggcgattggg ttagccgggc caaggccctg agtacggatg cggctggcgt ccacgccctg
     3241 ggtgatcaac gcgctggcaa cggaatccgc acgttgctgg gagagacgca tgttcaggtc
     3301 gtgaccaccc gtgctgtcgg tataaccaat cacgttaacc gccgttttcg gatactcttt
     3361 cagcaccatt gccacgccgg tcagggtgtt agcgcctgcc ggtttcaggg tcgcgctgct
     3421 gctgtcgaag gtcacattgt tcggcatatt gaggataatg ttatccccgc tgcgggttac
     3481 gctaacacca gtgccgcgca ttttgtcacg cagcttcgct tcctgcacat ccatgtaata
     3541 acccacgccg ccgcccagag ctgcgcctgc tgctgcgcca atcagcgcgc ctttaccgcg
     3601 atctttcttc gaagaagaga gcgcaccaat acccgcgccc acgagagagc ccagacctgc
     3661 gccgatagca gatttgcctg cttcgcgttc gccggtgtaa gggttagttg tgcagccaga
     3721 taccgccaga gcgccactca ctacggcggc aataagataa acacgtttct tcattgttaa
     3781 tccttaataa cctttttatt ctttgccacg ggttccgtgg cgggagatta tgccgcgtga
     3841 acatgaagat tattcctggg aatactcgga aatttgtaag taatatttaa ctgctcaata
     3901 catctaacct ttcaggagtc ttcggtttgg ccaactcatc ctcacgatat tccgttctga
     3961 ctgccgccca ctgggggccc atgctggttg aaaccgacgg cgaaaccgtg tttagctcgc
     4021 gtggcgcgtt agccacagga atggaaaact ccttgcagag cgcggttcgc gaccaggttc
     4081 acagcaatac gcgggtacga tttccaatgg tgcgcaaagg ctttctcgcg tcaccggaaa
     4141 acccgcaagg cattcgtggg caggatgaat ttgttcgcgt gagttgggat gaggcgctgg
     4201 atcttattca ccaacaacat aaacgcattc gtgaggctta tggtccggca tcgatttttg
     4261 ctggttccta cggctggcgt tcaaacggcg tgctgcataa ggcctcgaca ttattacaac
     4321 gctatatggc gctggcaggc ggttataccg ggcatctggg ggattattcg accggcgcgg
     4381 cacaggcgat catgccgtat gtcgtgggtg gtagcgaagt ttatcaacag cagaccagtt
     4441 ggccgctggt gctggaacat agcgatgtcg tggtgctgtg gagtgctaat ccactcaata
     4501 cgctgaaaat tgcgtggaat gcatccgatg agcaggggct ttcttacttt tctgcactgc
     4561 gtgacagtgg gaaaaagctg atctgcattg atccaatgcg atcggaaacc gtcgatttct
     4621 ttggcgataa aatggagtgg gtggcaccgc acatgggcac cgatgttgcg ctgatgctgg
     4681 ggatcgccca tacgctggtg gaaaatggtt ggcacgacga agcgtttctg gcgcgttgca
     4741 ccacaggtta tgccgtcttc gcctcttatt tgctgggcga gagtgacgga atagcgaaaa
     4801 ccgccgaatg ggcagcagag atttgtggtg ttggcgcagc gaaaatccgc gagctggcgg
     4861 ctattttcca ccaaaatacc accatgctga tggcaggctg gggaatgcag cgccaacagt
     4921 ttggtgagca aaaacactgg atgatcgtca cgctggcagc aatgttgggg caaatcggca
     4981 cacccggcgg cggttttggt ctttcttacc attttgccaa tggtggtaac cccacgcggc
     5041 gttctgcggt gctctcttcc atgcagggca gcttgccggg tggctgcgat gcggtagata
     5101 aaatccctgt ggcccgcatt gttgaagcac tggaaaaccc tggtggcgca tatcaacaca
     5161 acggtatgga ccgacatttt ccggatattc gttttatctg gtgggcgggc ggtgctaact
     5221 ttactcatca tcaggatacc aatcgtctga tccgtgcctg gcaaaaaccg gagctggtgg
     5281 tgatctctga atgcttctgg acggcggcgg cgaaacacgc ggatatcgtt ctgcctgcga
     5341 ctacctcttt tgagcgtaat gatctcacca tgaccggtga ttacagtaat cagcatctgg
     5401 tgccgatgaa gcaagtggtg ccgccacgct atgaagcgcg taatgatttt gatgtttttg
     5461 ccgagttgag tgaacgttgg gagaagggcg gctatgcgcg gtttacggaa ggaaaaagtc
     5521 agctgcaatg gctggaaacg ttttataacg ttgcccggca gcgcggggca agccagcagg
     5581 ttgaattgcc gccatttgct gagttctggc aagccaacca gttaattgag atgccggaaa
     5641 acccggacag cgagcggttt attcgcttcg ctgatttttg ccgcgatccg ctggcgcatc
     5701 cgttaaaaac cgccagcggc aagattgaaa tcttctcaca gcgtattgcc gattacggtt
     5761 acccggattg ccctgggcat ccaatgtggc tggagccgga cgaatggcag ggcaatgccg
     5821 aaccagaaca gttgcaggta ctttctgccc atccggcgca ccgcctgcac agccagctga
     5881 attacagttc tctgcgcgaa ttgtacgcgg tggcaaatcg tgagcctgtc accattcatc
     5941 ctgacgatgc ccaggagcgc ggcatacaag atggcgatac tgttcggttg tggaacgcac
     6001 gcgggcaaat tcttgccgga gcggtcatta gcgagggaat taaacctggc gtgatttgca
     6061 ttcacgaagg ggcatggccg gatctggatt taaccgctga cggtatttgt aaaaacggcg
     6121 cagtgaacgt gctgaccaaa gatctcccca gctcgcggct ggggaatggc tgtgcgggta
     6181 atacggcgct ggcatggctg gaaaaataca acggtccgga actgacactt acagcgtttg
     6241 aaccaccggc cagctcataa tccatgtggg tagttgggtt tcatcctgcc atgcgcaatc
     6301 gacaatgtga aaaccctgtg cctggtaaaa atttatcgcc ggttgatttt tttgataaac
     6361 ctccagcatc aggtggggat ggcgctgctg cacatactgc atcagcgcct taccaatacc
     6421 gcgcctgacg gccttcggtg cgacaaacat cgctgccaga aatcggcctt ccataatgct
     6481 gacaaaaccg agaagcttac cgtcttcttc ccagacccag ttttgcgcgt tggcaagata
     6541 ggcatcccgc accagcggaa tgcagtcacg ccagtaattc gcttttataa agggatgccc
     6601 ccaggttgta ctttccagcc acagttcgag gatcgcgggg agttctgaac gttgcgcttc
     6661 ccgaatcatg gtttatttcc cggatagcaa cagcagccaa ccacatgatc attcaccagc
     6721 ccacatgcct gcataaagga gtaacagatt gtggtgccga caaacttaaa accacgtttt
     6781 ttcagtgcct tagatagggc gtcggaggcg gacgtagatg tgggaatttc gctcaacgtt
     6841 gtggcttgtg tcacctgtgg ctgatgattt acaaacgacc agacaaagtc gacaaacggt
     6901 tcgccgttct gttccatttg caggtacgcc cgcgcattac caataattgc ctgaattttc
     6961 cctcgatggc ggataatccc ggcgtcctgt accagtcttt cgacatcctc ttcctgcatt
     7021 gctgcgacct tcaccggatc gaactgatga aagcaggcgc gatagttttc gcgttttttg
     7081 aggacggtga tccacgataa cccagcctgc tgcccttcaa ggcagatcat ttcgaacagt
     7141 tttttactgt cagtttcagg cacgccccac tcattatcat ggtaggcaat ataaagcggg
     7201 tcctgactca cccagccgca acgttccata ctttccctcg ctatgtaccc gatcgtaaaa
     7261 atttcagccg cttttcttga cgcatcggtg aaaaagtcaa tatttaatac agggctacta
     7321 tgcccgatat ccttcataca atgacaataa tattgccgaa ttcggctctt ccctggagtc
     7381 gtgttaatga tcataaaaaa aagcggtggt cgctggcagc taagcctgct ggcgagcgtg
     7441 gtaatcagtg ccttttttct caacacagct tacgcctggc aacaagaata tatcgttgat
     7501 acgcaacccg gacattccac agagcgttac acctgggata gtgatcatca acctgattac
     7561 aacgatattt tgtcgcaacg tattcaaagt agccaaaggg cgctgggact ggaagtcaat
     7621 ctggcggaag aaactcctgt ggatgtgacc agcagtatga gtatgggctg gaattttcct
     7681 ttatatgaac aggttacaac cggcccggtc gcggcattac attacgatgg cacaaccacc
     7741 tcgatgtata acgagtttgg cgacagtact accacgctga ccgatccgtt atggcatgcc
     7801 agcgtgagta gcttaggctg gcgtgttgac tcccggcttg gcgatctccg accctgggcg
     7861 caaatcagct ataaccagca atttggcgag aatatctgga aggcgcaatc aggcctgagc
     7921 cggatgacgg cgacaaacca gaacggcaac tggctggatg tcaccgtagg cgctgatatg
     7981 ttgctcaatc aaaatattgc cgcctatgcc gcgctatctc aggcagaaaa taccactaac
     8041 aatagcgact atctgtatac gatgggggtt agtgccagat tttaacgtaa aaatcacaat
     8101 tgaaaccatt ggataacaac agttgtggcg atagtgggtg ctaacttact aaataataaa
     8161 tttggtgaat aattgtcgcg tcattcattc ctgaactaag gcatttcatt ccgttctgat
     8221 ggcatttcat gccgtttttc cccaggcata aaatgcactt cgttatggtt gtcggcagag
     8281 atttttcctt tttattactg caggaatact gccatgacac cttcaaatta tcagcgtacc
     8341 cgctggctga cactcatcgg tactatcatt acccagtttg cactggggtc ggtttatacc
     8401 tggagcctgt ttaatggcgc gctttctgcc aagctggatg cgccggtaag ccaggtcgct
     8461 ttctctttcg gcttgttaag tctggggctg gcaatttcgt cttctgttgc gggcaaattg
     8521 caggaacgtt ttggcgttaa acgcgtcacc atggcttccg gcattttgct gggattaggc
     8581 ttcttcctga ccgcgcattc caacaacctg atgatgctgt ggttaagcgc cggtgtgctg
     8641 gtcggtctgg cggatggcgc gggttacctg ctgacgctct ctaactgcgt gaagtggttc
     8701 ccggagcgta aaggtctgat ctccgcgttc gctatcggtt cttatggtct gggtagcctg
     8761 ggtttcaaat ttatcgacac gcagctgctc gaaacggtgg gtttggaaaa aacatttgtg
     8821 atttggggag cgattgcgct ggtgatgatt gttttcggcg caacgttaat gaaagacgca
     8881 ccaaaacagg aagtgaaaac cagcaatggt gtggtggaga aagattacac gctggcagag
     8941 tcgatgcgta aaccgcagta ctggatgtta gcggtaatgt tcctgactgc ctgcatgagc
     9001 ggcctgtacg tgattggggt agcgaaagat atcgcccaaa gtctggcaca ccttgatgtg
     9061 gtttccgcag ccaatgcagt aacggttatt tccatcgcta acctttcagg tcgtctggtg
     9121 ctggggatac tgtctgacaa aattgcccgt atccgtgtta tcaccattgg tcaggtgatt
     9181 tcgctggtgg gtatggcggc cctgctgttt gcaccattaa atgcagtgac gttctttgca
     9241 gcgattgcct gcgtggcatt taactttggc ggcactatta ccgtctttcc gtcactggtc
     9301 agtgagttct tcggcctcaa taacctggcg aaaaactacg gtgtgattta cctcggtttc
     9361 ggtatcggta gcatttgtgg ttcgattatc gcctcactgt ttggcggctt ctatgtgacc
     9421 ttctacgtga ttttcgccct gctgattctg tcattggcgc tttctacgac aattcgtcag
     9481 ccagagcaga aagtattgcg tgaggcgcat atttagaaat atgttgctac tgaaataaat
     9541 gcgaggaggg agacttcaaa gtctcccttt ttttatggac gcatattaaa agaacaataa
     9601 tatttacacc acaaatatat tttatttgtt tatatatcat
//
LOCUS       DAZFQD010000174         9630 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.174, whole genome shotgun sequence.
ACCESSION   DAZFQD010000174 DAZFQD010000000
VERSION     DAZFQD010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9630
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.174"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(64..822)
                     /gene="yhdZ"
                     /locus_tag="ACHKTW_004564"
     CDS             complement(64..822)
                     /gene="yhdZ"
                     /locus_tag="ACHKTW_004564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFO6465468.1"
                     /translation="MSQILLQPANAMITLENVNKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIAVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQAAPDEFFAHPKSERTRAFLSQVIH"
     gene            complement(830..1933)
                     /gene="yhdY"
                     /locus_tag="ACHKTW_004565"
     CDS             complement(830..1933)
                     /gene="yhdY"
                     /locus_tag="ACHKTW_004565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFO6465469.1"
                     /translation="MTKVLLSQPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALQIGLVSIAPMFWKILPHRGRYIAAWAVIYPLIVWWLMYGGFFGLERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTSIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPRLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPTWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(1943..3124)
                     /gene="yhdX"
                     /locus_tag="ACHKTW_004566"
     CDS             complement(1943..3124)
                     /gene="yhdX"
                     /locus_tag="ACHKTW_004566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFO6465470.1"
                     /translation="MSHRRSTVKGSLSFANPTVRAWLFQILAVVAVVGIVGWLFHNTV
                     TNLNNRGITSGFAFLDRGAGFGIVQHLIDYQQGDTYGRVFIVGLLNTLLVSALCIVFA
                     SVLGFFIGLARLSDNWLLRKLSTIYIEIFRNIPPLLQIFFWYFAVLRNLPGPRQAVSA
                     FDLAFLSNRGLYIPSPQLGDGFIAFILAVVMAIVLSVGLFRFNKTYQIKTGQLRRTWP
                     IAAVLIIGLPLLAQWLFGAALHWDVPALRGFNFRGGMVLIPELAALTLALSVYTSAFI
                     AEIIRAGIQAVPYGQHEAARSLGLPNPVTLRQVIIPQALRVIIPPLTSQYLNIVKNSS
                     LAAAIGYPDMVSLFAGTVLNQTGQAIETIAMTMSVYLIISLTISLLMNIYNRRIAIVE
                     R"
     gene            complement(3192..4217)
                     /locus_tag="ACHKTW_004567"
     CDS             complement(3192..4217)
                     /locus_tag="ACHKTW_004567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO6465471.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPGFSYADADGKFSGIDVDVCRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAYNIIKQVGNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
     gene            complement(4648..4869)
                     /locus_tag="ACHKTW_004568"
     CDS             complement(4648..4869)
                     /locus_tag="ACHKTW_004568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6465472.1"
                     /translation="MKRLIPVALLTALLAGCAHDSPCVPVYDDQGRLVHTNTCMKGTT
                     QDNWETAGAIAGGAAAVAGLTMGIIALSK"
     gene            complement(5122..8226)
                     /gene="acrF"
                     /locus_tag="ACHKTW_004569"
     CDS             complement(5122..8226)
                     /gene="acrF"
                     /locus_tag="ACHKTW_004569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit AcrF"
                     /protein_id="HFO6465473.1"
                     /translation="MANFFIRRPIFAWVLAIILMMAGALAILQLPVAQYPTIAPPAVS
                     VSANYPGADAQTVQDTVTQVIEQNMNGIDNLMYMSSTSDSAGSVTITLTFQSGTDPDI
                     AQVQVQNKLQLATPLLPQEVQQQGISVEKSSSSYLMVAGFVSDNPDTTQDDISDYVAS
                     NVKDTLSRLNGVGDVQLFGAQYAMRIWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQ
                     LGGTPALPGQQLNASIIAQTRLKNPEEFGKVTLRVNSDGSVVRLKDVARVELGGENYN
                     VIARINGKPAAGLGIKLATGANALDTAKAIKAKLAELQPFFPQGMKVLYPYDTTPFVQ
                     LSIHEVVKTLFEAIMLVFLVMYLFLQNMRATLIPTIAVPVVLLGTFAILAAFGYSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMMEDKLPPKEATEKSMSQIQGALVGIAMVLS
                     AVFIPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATLLKPTSAEHHENK
                     GGFFGWFNTTFDHSVNHYTNSVGKILGSTGRYLLIYALIVAGMVVLFLRLPSSFLPEE
                     DQGVFLTMIQLPAGATQERTQKVLDQVTDYYLKNEKANVESVFTVNGFSFSGQAQNAG
                     MAFVSLKPWEERNSDENSAEAVIHRAKMELGKIRDGFVIPFNMPAIVELGTATGFDFE
                     LIDQAGLGHDALTQARNQLLGMAAQHPASLVSVRPNGLEDTAQFKLEVDQEKAQALGV
                     SLSDINQTISTALGGTYVNDFIDRGRVKKVYVQADAKFRMLPEDVDKLYVRSANGEMV
                     PFSAFTTSHWVYGSPRLERYNGLPSMEIQGEAAPGTSSGDAMALMENLASKLPAGIGY
                     DWTGMSYQERLSGNQAPALVAISFVVVFLCLAALYESWSISVSVMLVVPLGIVGVLLA
                     ATLFNQKNDVYFMVGLLTTIGLSAKNAILIVEFAKDLMEKEGKGVVEATLMAVRMRLR
                     PILMTSLAFILGVLPLAISNGAGSGAQNAVGIGVMGGMVSATLLAIFFVPVFFVVIRR
                     CFKG"
     gene            complement(8238..9395)
                     /gene="acrE"
                     /locus_tag="ACHKTW_004570"
     CDS             complement(8238..9395)
                     /gene="acrE"
                     /locus_tag="ACHKTW_004570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrE"
                     /protein_id="HFO6465474.1"
                     /translation="MTKHARFFLLPSFILISAALIAGCNDKGEEKAHVGEPQVTVHIV
                     KTAPLEVKTELPGRTNAYRIAEVRPQVSGIVLNRNFTEGSDVQAGQSLYQIDPATYQA
                     NYDSAKGELAKSEAAAAIAHLTVKRYVPLVGTKYISQQEYDQAIADARQADAAVIAAK
                     ATVESARINLAYTKVTAPISGRIGKSTVTEGALVTNGQTTELATVQQLDPIYVDVTQS
                     SNDFMRLKQSVEQGNLHKENATSNVELVMENGQTYPLKGTLQFSDVTVDESTGSITLR
                     AVFPNPQHTLLPGMFVRARIDEGVQPDAILIPQQGVSRTPRGDATVLIVNDKSQVEAR
                     PVVASQAIGDKWLISEGLKSGDQVIVSGLQKARPGEQVKATTDTPADTASK"
BASE COUNT         2556 a         2490 c         2301 g         2283 t
ORIGIN      
        1 catcaaatca agcgaaaggc catccgaaag gatggccttt ttgcttttcg aactaacatt
       61 caattaatgg attacctgcg ataaaaatgc cctcgtacgc tctgatttag gatgcgcaaa
      121 aaattcatca ggtgcagctt gttccactat ctccccacga tccataaaaa ttacccggtc
      181 agcgacggta cgtgcaaacc ccatctcatg tgttacacac aacattgtca tacccgactg
      241 cgccagccca atcatcgtat ccagcacctc tttcaccatc tccggatcga gcgccgacgt
      301 tggctcatca aacaacataa ttttcggctt catacacagc gaacgcgcaa tggcaacgcg
      361 ttgctgctga ccaccagaaa tttgtccggg aaacttatgc gcatgttcgg caattctcac
      421 ccgctcaagg taatgcatcg ccagagcttc agcctctttc tttggcatct tgcgtaccca
      481 aatcggtgcc agggtacagt tctgtagaac ggtcagatga gggaagagat tgaaatgctg
      541 aaagaccatt cccacttcct gccggacgcg ctcaatattg cggatatctt cattaagttc
      601 gatgccatct accgcgatcc gtccctgttg atgttcttcc agatgattaa tacaacgaat
      661 ggttgtcgat ttaccggaac ctgaagggcc acacagaacg atccgttctc ccggttgcac
      721 ggttaaattt atatttttca aaacatggaa ttgtccatac catttattga cgttttccag
      781 cgtaatcatc gcgttagcag gttgcagtaa aatttggctc atagtgtcct caatgcggtg
      841 tacgcccggt gttaaaacgt ttttccagat gctggctata gcgcgacatg ctgaaacaga
      901 agatccagta gatcagcgcg gcgaaaacat acccttccgt cgacataccc aaccaggtgg
      961 gatcaacggt tgcctgctga acgctactga aaagatcgaa caacccgata atgatcacca
     1021 ggctggtatc tttgaagagt gcgatgatgg tatttaccag cctaggaatt accagcttca
     1081 acgcctgtgg cagaataacc agcccctgag ttttccagta acccaacgcc agcgactctg
     1141 ccgcttcata ctgcccttta ggcagtgcct gtaatccgcc tcgcacgact tccgcaacat
     1201 atgctgactg aaacaggatc acgccaacca gcgcgcggat cagtttgtcg atactggtgc
     1261 cttctgccat aaacaacggc agcatgaccg aagacataaa caatacggta atcagcggta
     1321 cgccgcgcca gaactcgata aaaatgaccg ataagatacg cacaatcggc atatgggagc
     1381 ggcgacctaa cgccagtaag atcccccacg gtagcgcccc cgcaatccca acggatgcaa
     1441 taattaaagt tagcgtcagc ccgccccatt gccgggtttc aacccgctcc agaccaaaaa
     1501 aaccgccata catcagccac cagacaatca gtgggtaaat caccgcccag gccgcaatat
     1561 agcgaccgcg atgcgggagt attttccaga acattggtgc gatcgacaca agcccaatct
     1621 gtaatgcgag gttaattcgc cagcgttggt cgtgtgggta aagcccatac ataaactgac
     1681 caaatcgttc gtggatgaag acccaacagg caccggcttt tgtgcagtcg gcacgcgttg
     1741 agccaaccca gttagcctgc aaaaatgccc agttcagcaa cggtgggatc agttcccaca
     1801 tcaaccatat gcagccaata gtcagcaggc tattgctcca gctggagaac agattttttc
     1861 gcacccacac catcgcgcgg cttgagttat ggctcgctgg gcgcgagggc tgagacagca
     1921 atacttttgt catcatgact ccttagcgtt caacgatcgc gatgcggcgg ttatagatat
     1981 tcatcagcag cgagatagtc aggctgataa tcagatagac cgacatggtc atggctatcg
     2041 tctcgatggc ttgccccgtc tggttcagca cggtgccagc aaacagcgaa accatatcgg
     2101 gatagccaat agcggcggca agagaggagt ttttgacgat gttgagatac tggctggtta
     2161 acggcggaat aatcactcgc agtgcctggg gaataatgac ctggcgtagc gtaaccgggt
     2221 tgggtaatcc cagtgaccga gccgcttcat gttgaccata aggcactgcc tggatcccag
     2281 cgcggataat ctcggcgata aatgcagatg tataaaccga aagtgccagc gttaaggctg
     2341 ccagttcagg aattaaaacc atcccgccgc ggaagttaaa gcctcgtagg gctgggacat
     2401 cccagtgtag tgctgcgcca aaaagccatt gcgccagtaa aggcaaacca atgatcaaca
     2461 ctgcggcgat cggccaggtg cggcgcagtt gtccggtctt tatctggtat gttttattaa
     2521 agcggaatag cccaacagaa aggactatag ccataacaac agccaggata aacgcaataa
     2581 atccgtctcc cagctgcggt gacggaatat aaagcccacg attgctcaaa aaggccagat
     2641 caaacgcgct cacagcctgg cgtggtccgg gcaaattgcg caacacggca aagtaccaga
     2701 agaagatttg cagcagcggg ggaatattac ggaagatctc aatataaatt gtggatagtt
     2761 ttcgtagcag ccagttatcc gaaagtctcg ccagaccgat aaagaagcct aaaacagaag
     2821 cgaacacaat acacaatgca gaaaccagta gcgtattgag taagccgaca ataaaaacgc
     2881 gtccgtaggt gtcgccctgc tggtaatcga tcaaatgctg gacaataccg aagccagcgc
     2941 cgcgatccag aaaggcaaaa cctgaagtaa tgccacgatt attgagattc gttacagtgt
     3001 tgtgaaataa ccaaccaaca atgccgacaa cagcaacaac ggcaaggatc tggaataacc
     3061 aggcgcgaac cgtagggttg gcaaaagaga gtgagccttt aacggttgag cggcgatgag
     3121 acatagaaac ctcggaaagc ataacgctga agagagcgcc gccgtagcga cgcccgttac
     3181 ctcacaccga cttaacgcac gggcggcgcg tactgaatac cgccgttatt ccagagatta
     3241 ttttgcccac gtttaatttt cagcgggctt tctgaaccta cgttacgctc gaaaatttcc
     3301 gagtagttac ccacctgttt gatgatgtta taggcccatt tattatccag cttcagatcc
     3361 ttgccgtaat cgccttcttt acccagcaga tgtgccatat caggcgttgc tggattagcc
     3421 gctttttcat cgacgttctg ggaattgatg cccatctctt cagcattcag catggcgaaa
     3481 agcgtccagc gtacaatcga gaaccattca tcatcgccac gacgaactac cggcccaagc
     3541 ggttctttag agataacctc cggtaagaca atccattcag ctgggttgct taatttgatg
     3601 cgcagggcat acagttgcga ctgatccgag gccagcgtat cgcagcgacc agattccagt
     3661 gccttcgctg attcgtcaga gcgatcgaaa gtcaccggtg tgtacttcat attgtttgcc
     3721 ttgaaatagt cggcaacgtt gagttcggta tcagtacccg cctgaataca gacggtagcg
     3781 ccatccagtt ctttcgcgct ttttagcccc gctttatcgt gcgtcaggaa gccaatgccg
     3841 tcgtaataag tgacgccggt aaatgccatt cccatcccgg catcgcgaga tgaagtccag
     3901 gtcgtattac gggagagcaa atccacttcc cctgactgta aagcggtgaa gcgttctttt
     3961 gctgtgagtg gggtatattt cactttcgtg tcgtcaccaa atacagcagc ggcaacacca
     4021 cgacaaacat caacatcaat acctgaaaac ttaccgtctg catcggcata agagaaccca
     4081 ggtaatccat cactgatccc gcattgcaca aaaccttttt tctgaacggc atcaagcgtc
     4141 gcgccagcat gcgcctgatt tgcaacggca agcagcacgc tggcggcagc cagtgtggct
     4201 atcatcatct ttttcataat gcttcctgtg aggcgaaaat tatcgttata ggacgtttgg
     4261 gaaacgtctt ttacctgtct gtggccatcc tcttataagc aaaagtagtg ccagcttttg
     4321 cgcccgattt cgcgtcgcaa taagctgagt gtagacagga aataataaaa acagcgcccc
     4381 gcaatggtgc gaaaatgaaa catgcgccac attttggcgc aaaatattta cgcgtgtttt
     4441 actaagcaga ataatgattg ttagcgtgag tatatttctg gtgggaagtg tgaaagttaa
     4501 attgatgcgt tatttcgaaa gcagcgaaat aacgcgatat aacatgctaa atcagcagag
     4561 aattagccag cagaatatag caatatttta tcttatgtaa agatactgta tcgttaaaca
     4621 gagcgcgaaa ctccgtgccc tgttcagtta tttcgacaga gcgatgatcc ccatcgtcag
     4681 gccagcaaca gccgctgccc cgccagcaat agccccagcc gtttcccagt tatcctgagt
     4741 cgtgcctttc atacaggtat tggtgtgaac cagacgaccc tgatcgtcat aaaccggaac
     4801 gcagggcgaa tcgtgagcac agcccgccag caatgcggtg agcaatgcca caggaattaa
     4861 tcttttcatg gtgttacctc agtaataaac gctaacgctt aataagttaa ctctcttatt
     4921 aacgagctga atgccatttt atcaccggag gttataaatc ttgcggaatg aataatgaca
     4981 aattatgtga attgtaaaaa tagtgaaggg agcgggaact atagaaagct tttaaagagt
     5041 gagcagaaga atatcttatt aatttttatg ggtttcactg gaaataataa aggcacccga
     5101 aagcgccttt atgtttctga tttatccttt aaagcaacgg cggatcacca caaagaacac
     5161 cggtacgaag aagattgcca gcaacgttgc agagaccatt cctcccatta ccccgatacc
     5221 cacagcgttc tgcgcgccac tgccggcacc gttactgata gctagcggta atacgccgag
     5281 aataaaggcg agagaggtca tcaggatagg acgcagacgc atacgtactg ccatcagtgt
     5341 cgcttcaaca acacctttac cctctttctc catgagatct ttagcgaact caacaatcaa
     5401 aatagcgttt ttggccgaca agccaattgt cgttagcaag cccaccataa agtagacgtc
     5461 atttttttga ttaaagagtg ttgccgccag tagcacgccg acaatcccta acggcactac
     5521 caacataacc gagacagaaa ttgaccagct ttcatagagt gcagcaaggc acaggaaaac
     5581 aaccacaaag gaaattgcta ccagagcggg agcctggttt cccgataagc gttcctgata
     5641 cgacataccc gtccagtcat aaccaatgcc cgcaggtaat tttgacgcaa ggttttccat
     5701 caacgccatg gcatcgccgg aactggttcc tggcgcggct tccccctgaa tctccattga
     5761 cggcagaccg ttgtagcgtt ccagtcgcgg agagccatac acccaatgtg aagtggtaaa
     5821 ggccgagaat ggcaccattt cgccgttggc gctgcggaca taaagtttat cgacatcttc
     5881 tggcagcata cggaatttgg catccgcctg aacatacacc tttttcacgc gaccacggtc
     5941 gatgaagtcg ttaacgtaag tcccacccaa cgccgttgaa atggtctgat tgatgtcaga
     6001 aagtgaaaca cctaatgcct gcgccttttc ctggtcaact tccagtttaa actgcgcggt
     6061 gtcttccagg ccattaggac gcacgctgac taagctggca ggatgttgcg ccgccatgcc
     6121 aagcaactgg ttacgggcct gggtcagggc atcgtgaccc agcccagcct gatcaattaa
     6181 ctcaaagtcg aaacccgttg ccgtgcccag ttcaacaatg gctggcatat tgaatggaat
     6241 gacaaaaccg tcgcggatct tgcccaattc cattttggca cgatggatta ccgcttccgc
     6301 actgttttca tcactattac gctcttccca cggtttcaga ctgacgaagg ccataccggc
     6361 gttttgtgcc tggccgctga agctaaagcc gttaaccgta aagacacttt caacgttcgc
     6421 tttctcgttc ttcagatagt aatccgtcac ttgatccaac actttttgcg tccgctcttg
     6481 cgtcgcgcca gcgggtaact gaatcatggt cagaaagaca ccctgatcct cttcaggtaa
     6541 gaaggaagac ggaagacgta aaaacaacac caccattcct gcaacaatca gcgcatagat
     6601 cagtaaatat cgtcctgtgg aaccgaggat tttgccgacg ctgttggtgt agtggttaac
     6661 gctatgatcg aaggtggtat taaaccagcc gaagaaacca cccttatttt cgtgatgctc
     6721 agctgaggtg ggtttgagca gtgttgcaca taacgcaggg gtaagaatca atgccaccag
     6781 aacagaaagc gccattgccg aaacgatggt gatagagaac tggcgataaa ttgccccagt
     6841 agaaccgccg aagaatgcca tcggaataaa taccgctgac agcaccatcg cgatacccac
     6901 cagtgcgccc tgaatttgcg acatcgattt ttccgtcgct tcttttggcg ggagcttatc
     6961 ctccatcatc acgcgctcga cgttctccac caccactatc gcatcatcga cgagcagccc
     7021 gatggcaagc accatcccga acatcgttag tgtgttgatg gagtaaccaa aagcggcgag
     7081 gatggcaaac gtccctaata acaccacggg taccgcaatg gtggggatca gcgttgctcg
     7141 catattctgc aagaacagat acatcaccag gaacaccagc ataatggctt cgaacagcgt
     7201 ttttaccact tcgtgaatag aaagctggac gaatggcgtg gtgtcataag ggtagagaac
     7261 cttcattccc tgcgggaaga atggctgtaa ttccgccagt tttgccttaa tggctttcgc
     7321 ggtatcgaga gcattcgcgc cggttgccag cttaatcccc aggcccgccg ccggttttcc
     7381 gttgatacga gcgataacgt tatagttttc accgccaagt tcaactcgtg cgacatcttt
     7441 caggcgtacc accgagccgt cactgtttac gcgcagggtt actttgccga attcttccgg
     7501 attttttaac cgcgtctgag caataattga ggcgttcaac tgttgccctg gtaacgctgg
     7561 cgttccgccc aactgtccgg cagcgatctg atcgttctgt accttcaact ggttaatcac
     7621 atcaaccggt gtcagtttat atttgtttag cagatcggcg tccagccaga tacgcatcgc
     7681 atactgtgcg ccgaaaagct gtacgtcacc gacgccattc agacggctaa gcgtatcttt
     7741 aacgttagag gccacatagt ccgagatatc gtcctgtgtg gtgtctgggt tatcagagac
     7801 aaagcccgcc accatcaaat agctgctact ggacttttca acactgatcc cctgctgctg
     7861 aacctcctgc ggcagcaacg gcgtggcgag ctggagtttg ttctgcacct gcacttgcgc
     7921 gatatcagga tcggtcccgg actggaaggt aagggtaatt gtcacgctac cggcggaatc
     7981 gctggtggag gacatataca tcaggttatc gataccgttc atattctgtt cgataacctg
     8041 cgtcaccgta tcctgcacgg tctgcgcatc agcgcccgga tagtttgctg aaacagaaac
     8101 cgcaggcggt gcaatggttg gatactgagc gacgggtaat tgtaggatcg ccagtgcgcc
     8161 cgccatcatc agaataatgg ccagcaccca tgcaaatatc ggtcgtcgaa taaaaaagtt
     8221 tgccatgtca gattacctta cttcgatgca gtatctgcgg gggtatcggt agtggcttta
     8281 acctgctctc ccggacgcgc tttttgcagg ccgctgacaa tgacttgatc gccagatttc
     8341 agaccttcgc taatcaacca tttatcgcca atcgcctgac tggcaacgac agggcgcgct
     8401 tccacctgac ttttatcgtt aacaatcagc acggttgcat caccacgcgg tgtgcggcta
     8461 acgccttgtt gcgggataag aatggcgtca ggttggacgc cttcatcaat ccgtgcgcgc
     8521 acaaacatac ccggcaaaag cgtatgttgc gggttaggga agacggcacg tagggttatg
     8581 gagccggtgc tttcatcaac ggtcacatca gagaattgca gcgtaccttt caggggatag
     8641 gtttgaccgt tttccatgac caactctacg ttgctggtgg cgttttcctt atgcaaattt
     8701 ccttgctcta cggattgctt cagcctcata aaatcgttgc tggattgggt cacatcaacg
     8761 tagataggat cgagctgctg gacagtcgcc agttcagtcg tttgcccatt agtgacaaga
     8821 gcgccttcgg tcacggtcga tttgccgata cgtccgctaa ttggcgcagt cactttggta
     8881 taagcgagat tgatgcgagc gctttcgact gtggcttttg cggcaatcac ggcggcatcg
     8941 gcctggcgag catcagcaat ggcctggtcg tactcctgct ggctgatgta tttcgtaccc
     9001 acgagcggaa cgtaacgttt taccgtcaaa tgcgcgatgg cggcggcggc ttcacttttc
     9061 gccagttcgc ctttcgcgct gtcataattt gcctgatagg tcgcgggatc gatctggtac
     9121 agggactggc ctgcttgtac atcgctgcct tcagtgaaat tacgattcag tacgatcccg
     9181 ctaacctgtg ggcgaacttc ggctatacgg taagcattgg tgcggcctgg taattcagtc
     9241 ttaacttcta acggggccgt tttcacaata tgaacggtaa cctgcggttc accgacgtga
     9301 gctttctctt ctcccttatc gttacaaccg gcgattaaag ccgcggagat cagaataaag
     9361 gagggcagga ggaaaaacct ggcatgtttc gtcattacta ttcctcaaaa aaccaaaagc
     9421 gcgttattta cccagaaagg caacgcgtta actcgcagaa agaaaaatac agtttgctat
     9481 cctacaaatt atcattcgtc gatgtaagga atagttatga atactggcgt ctcaaggcac
     9541 ataaacacaa aaaaagatta atattctact gttttatttt gacgcgggtt gaaagaggca
     9601 gaattaaaac ctcgtaaatt gaaatatata
//
LOCUS       DAZFQD010000175         9582 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.175, whole genome shotgun sequence.
ACCESSION   DAZFQD010000175 DAZFQD010000000
VERSION     DAZFQD010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9582
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.175"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..2223
                     /locus_tag="ACHKTW_004571"
     CDS             <1..2223
                     /locus_tag="ACHKTW_004571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HFO6465475.1"
                     /translation="RWDEESASFVPVEPESDPMNVTFDNLAPEVQNAVMVKFDACENI
                     TVDMVISAQELLQEDMATFDGHIVEALMKMPEVNAMYPELKLHAIGWVKHKCKPAAKW
                     PEIQAEMRIWKKRRESERKETGKYISVVDIARSRVNQQNTENAAEKTGAVTVAVRREY
                     KQTWKTLDNELACALWPGDVDAGNIDGTIHRWATNEVIDKDREDWKRISASMRKQPEA
                     LAYDRQTIFGLVRERPIDIHKDPVALNKYISEYLTTKGVFEHEETDQSSADALQSPAA
                     QTAPVETAESDTQKNEILVEAEPSVEREGPFYFVFTDKGGEKYGRANKLSGLDRALAA
                     GGTEISKEEYFARKNGTYTGLPQNVDSTEDSEQPEPVKVTADEVNKIMQAANISQPDA
                     DKLLAASRGEFVEGISDPDDPKWVKGIQSRDAEDQNQPNVKQNEPEAEQNSPDTQQNG
                     PEEQQPEPAVQQELEKVCTACGQTGGGNCPDCGAVMGNATYQETFDEENQAEAQENDP
                     EEMEGTEHLHKENTVSDQYHASDNKTGETANPLIKVNGHHETTSTSRLWHHLMIDLET
                     MGKNPDAPINAIAGKFFDPATGEMGPEFSKTIDLETAGGVIDRDTIKWWLKQSREAQS
                     AILTDEIPLDDALLQFREFIDENSGEFFVQVWGNGATFDNVILRRSYERQGIPCPWRY
                     TNDRDVRTMVALGLVMDFDARTTIPFEGERHNALHDARYQAKYVSAIWQKLIPSQADF
                     "
     gene            2288..2536
                     /locus_tag="ACHKTW_004572"
     CDS             2288..2536
                     /locus_tag="ACHKTW_004572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFO6465476.1"
                     /translation="MARLILLTEWAKEEFSDPVPTPGTLSKYAKAGMIFPLPKKVGRH
                     WRVDPRARFVGMVNKPEVIATDHPALKRILEDGAPTKI"
     gene            2514..3644
                     /locus_tag="ACHKTW_004573"
     CDS             2514..3644
                     /locus_tag="ACHKTW_004573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008907 - integrase activity [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
                     /protein_id="HFO6465477.1"
                     /translation="MARPRKYKTEVPGLSPYFDKRNNKVYWRYRHPITGKNHGLGSID
                     QKQAEAIAAEANSRLARQQMEQMLSLQEKIISDTGSPSTVSAFLNSYRKIQQERYENG
                     EIKLNTLKQKASPLRVFDERFGTRPLDAITVKDVVSVLEDYKAKGHNRMGQIFRKVLI
                     DVFREAQQTGDVPLGFNPAESAKKPQVRISRQRLTFDEWMMIYNAAEKDGYFLQRGML
                     LALMTGQRLSDICKMKFSDIRDGYLHVEQQKTGTRIAIPLALRYDKLNLTLDDVVSSC
                     RDCVLSPWLLHHHHAKGTAKRGGMVKPATLTVAFKKARDSVDYNWRANGTPPSFHEQR
                     SLSERLFREQGIDTKILLGHSNQKMTDIYNDARGKEWKKLVI"
     gene            complement(3690..4343)
                     /gene="ompW"
                     /locus_tag="ACHKTW_004574"
     CDS             complement(3690..4343)
                     /gene="ompW"
                     /locus_tag="ACHKTW_004574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HFO6465478.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTMAKYKSDGRKLAKGSYSDNVRLDPWVFMFSAGYRF"
     gene            4583..4672
                     /locus_tag="ACHKTW_004575"
                     /pseudo
     CDS             4583..4672
                     /locus_tag="ACHKTW_004575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            4700..5443
                     /gene="yciC"
                     /locus_tag="ACHKTW_004576"
     CDS             4700..5443
                     /gene="yciC"
                     /locus_tag="ACHKTW_004576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciC family protein"
                     /protein_id="HFO6465479.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSGLIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGIFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAILLIYLFRLYMLIRQ"
     gene            5473..6012
                     /gene="yciB"
                     /locus_tag="ACHKTW_004577"
     CDS             5473..6012
                     /gene="yciB"
                     /locus_tag="ACHKTW_004577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HFO6465480.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLGKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            6117..6515
                     /gene="yciA"
                     /locus_tag="ACHKTW_004578"
     CDS             6117..6515
                     /gene="yciA"
                     /locus_tag="ACHKTW_004578"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HFO6465481.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(6555..7274)
                     /gene="tonB"
                     /locus_tag="ACHKTW_004579"
     CDS             complement(6555..7274)
                     /gene="tonB"
                     /locus_tag="ACHKTW_004579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HFO6465482.1"
                     /translation="MTLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAPAQP
                     ISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKP
                     VKKVQEQPKRDIKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRALSRNQ
                     PQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRYEPG
                     KPGSGIVVNILFKINGTTEIQ"
     gene            7498..7794
                     /gene="yciI"
                     /locus_tag="ACHKTW_004580"
     CDS             7498..7794
                     /gene="yciI"
                     /locus_tag="ACHKTW_004580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HFO6465483.1"
                     /translation="MLYVIYAQDKADSFEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
     gene            8093..9346
                     /gene="kch"
                     /locus_tag="ACHKTW_004581"
     CDS             8093..9346
                     /gene="kch"
                     /locus_tag="ACHKTW_004581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HFO6465484.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLVKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESVQK"
     gene            complement(9401..9574)
                     /gene="yciY"
                     /locus_tag="ACHKTW_004582"
     CDS             complement(9401..9574)
                     /gene="yciY"
                     /locus_tag="ACHKTW_004582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HFO6465485.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
BASE COUNT         2590 a         2225 c         2289 g         2478 t
ORIGIN      
        1 cgctgggatg aggagtctgc gtcctttgtc ccggtcgaac ccgaatccga tccgatgaac
       61 gtcacttttg acaatctggc ccctgaagta cagaacgccg ttatggttaa gttcgacgca
      121 tgtgaaaaca tcactgttga tatggtgatt agcgctcagg aattgttgca ggaagacatg
      181 gcaacattcg acggccatat cgttgaagcg ttgatgaaga tgccagaagt taacgccatg
      241 tatccggagc ttaagctgca tgccatcggg tgggttaagc ataaatgtaa acctgctgct
      301 aaatggcccg aaattcaggc agagatgcgc atctggaaaa aacgtcgcga aagtgaacgc
      361 aaggaaaccg ggaaatacat atctgtcgtt gatatcgccc gttccagagt aaaccaacag
      421 aacactgaaa acgctgctga aaaaaccggg gctgtcactg ttgccgttcg ccgcgaatac
      481 aaacagacat ggaaaacact cgacaatgaa ctggcctgcg ccctctggcc cggtgatgtg
      541 gatgcaggaa acattgacgg taccatccat cgctgggcga caaatgaggt tatcgacaag
      601 gatcgcgaag actggaagcg tatctcagca tcaatgcgca aacagcccga agcacttgcc
      661 tatgaccgtc agactatttt tggccttgtt cgcgaacgtc cgatcgatat tcacaaagat
      721 cccgttgcac tgaacaaata tatcagcgaa tacctgacga caaagggcgt gtttgaacat
      781 gaagaaacag accagagctc tgctgatgct ctccagtcac cagcagcaca aactgctcca
      841 gtggagacgg cagaatccga tactcaaaaa aatgaaatcc tggtggaagc tgaaccatct
      901 gtagagcgtg aaggaccatt ttatttcgtc tttaccgata agggcgggga aaaatacggc
      961 agggcaaaca aactttctgg tctggacagg gcgctggctg ccggcggtac cgaaatctca
     1021 aaagaagaat attttgcccg aaaaaatggc acatacacgg gcttaccgca aaatgtggat
     1081 agcactgaag attccgaaca accagagccg gtaaaagtta ccgctgacga agtaaacaaa
     1141 attatgcagg cagccaatat cagccagcct gacgccgata aattgcttgc tgcatcacgt
     1201 ggtgaatttg ttgaagggat tagcgacccg gatgatccga aatgggtgaa agggatccag
     1261 agtcgcgacg ctgaggacca gaatcagccc aacgtgaaac aaaatgagcc agaagcggaa
     1321 caaaacagcc cggatacgca acaaaacggg ccagaagaac aacaaccaga accagcagtg
     1381 caacaggaac tggaaaaagt ttgcaccgca tgcggtcaga ccggtggcgg caactgtcct
     1441 gactgtggtg cggtgatggg gaacgcaacc taccaggaaa cattcgatga agaaaatcag
     1501 gctgaagctc aggaaaatga tccggaggaa atggaaggca ctgaacatct gcacaaggag
     1561 aacactgtca gcgatcagta tcacgccagc gataataaaa ctggcgaaac agcaaatccc
     1621 ttaattaaag tgaacggtca tcatgaaacc acatccacca gcaggttgtg gcaccatctg
     1681 atgattgacc ttgaaacaat gggaaaaaat cctgatgcgc caataaacgc tatagccggt
     1741 aagttttttg atccggcaac cggagagatg gggccagaat tcagcaaaac tatcgatctg
     1801 gaaaccgcag gcggggtcat cgatcgggac accattaagt ggtggctgaa acagtcacgc
     1861 gaagcacaat ccgccattct gaccgatgaa atcccgttgg atgatgcact gctgcaattc
     1921 cgggaattta tcgacgaaaa ctccggtgaa ttttttgttc aggtctgggg taacggtgca
     1981 actttcgaca acgtgatttt acgccgttca tatgaacggc aggggatccc ctgcccgtgg
     2041 cgttacacca atgatcgcga tgtaagaacg atggttgctc tgggactggt gatggatttc
     2101 gacgcaagaa cgactattcc attcgaaggt gaacgccata acgcgctgca cgatgcgcgt
     2161 taccaagcaa aatacgtttc agccatctgg caaaaactga tcccgagtca ggctgatttt
     2221 taatgttcaa ccccggtcgt cgcccgcaag ctatagtggc ggcgaccatg attagcgaac
     2281 aactctcatg gcaagactta ttcttctcac tgagtgggca aaagaggaat tcagtgaccc
     2341 ggtcccgact ccgggcacgt taagtaaata cgctaaagcc ggaatgatat ttcctctccc
     2401 caaaaaagtt ggaagacact ggcgagtgga tcctcgagct cgctttgtcg gaatggtaaa
     2461 caagccggaa gtgatcgcca cagatcaccc tgctttgaag aggatactgg aagatggcgc
     2521 gcccacgaaa atataaaact gaagttccgg gattatctcc gtattttgac aaaagaaata
     2581 acaaagttta ctggcgttac aggcatccca taacaggaaa aaatcacgga ctcggcagta
     2641 ttgaccagaa acaggcagaa gctattgcag cagaagcgaa cagccgtctt gccaggcaac
     2701 aaatggaaca aatgctcagt ctgcaggaga aaattattag tgataccggt agtccatcaa
     2761 ccgtttctgc ttttctgaat agttaccgaa aaattcaaca ggaaagatat gaaaacggag
     2821 agatcaaact caacacactg aaacagaaag catcccctct cagagtattt gatgaacgtt
     2881 ttggtaccag accattagat gccataaccg taaaagatgt ggtatcggtg ctggaagatt
     2941 acaaggcaaa aggacataac agaatgggac aaattttcag gaaagtactg atcgatgttt
     3001 tccgggaggc ccagcaaacg ggcgatgtcc cgctaggctt taaccctgca gaatcggcaa
     3061 aaaaaccaca ggtacggata tcacggcaac gactgacctt tgatgagtgg atgatgattt
     3121 ataatgcagc ggaaaaggat ggttactttt tacagcgtgg tatgctgctg gcactgatga
     3181 caggccagcg cctttcagat atttgcaaaa tgaaattttc ggatatcagg gatggttatc
     3241 ttcatgtcga acagcaaaaa acaggaaccc ggattgccat ccctctggct ctgcgttacg
     3301 ataaattaaa tctcaccctg gatgatgtgg tgtcatcctg tcgcgattgc gttcttagtc
     3361 cgtggctatt gcaccaccat catgctaaag ggacagctaa gcgcggcggg atggttaagc
     3421 cagcaacgtt aaccgttgca tttaaaaaag cccgggattc tgtggattac aactggcgtg
     3481 ctaatggcac cccaccctct ttccatgagc agagatcttt atcagaacga ttgttcagag
     3541 aacagggaat tgataccaaa attttgctgg gccattcgaa tcaaaaaatg accgatattt
     3601 acaacgacgc acgcggtaag gaatggaaaa aactggtcat ttgatgacca gttttgcaga
     3661 agagttttgc agaggttttg cagagcaaat taaaaacggt accctgcaga gaacataaac
     3721 acccacggat caaggcgtac attgtcgcta taactgcctt tagccagttt acgaccatca
     3781 gatttatact tagccatggt atcgatatcc atgtaccaga ctgacatgtt aaccaaccag
     3841 tcacggttaa tcagataatc aacccccacc tgcccggcag ctccccagga atctttcaga
     3901 ctgagatcgg aaagccctgc ctctttgcca tgatcgttaa atccattatc aaagaaggtg
     3961 gtgtagttaa taccggcccc aacgtaagga cggaatttgc tgctggcatc accaaaatac
     4021 cactgcgcca tcagtgttgg cggcagatga tgaacggttg caatatcgcc ggtcgcccgg
     4081 gtgccgattt tatggcggaa cggcgtcgct gccagtaatt ccacaccaat gttgtcggtc
     4141 gccatataag taaacgtcag gcccagttga gtgttattgg tcacgctgaa tccacccaga
     4201 cttcctaacg taccaccagc accttctgtt ggacgtacgg ttgcagaacc tgcacgcata
     4261 aaaaattcgc ctgcttcatg cgcaaaggca ctgccagaga gaagagttgt tactgccaaa
     4321 gccgccactg ttaacttttt catatccgct ccgtcgttat ggttataaaa acgtgacgaa
     4381 tatacctaca ttggagtaac aaatgatcct acatagatca cattacatat gataattcac
     4441 catttattga tctggattaa tttagaagtt gcattgagaa tcctggttaa cgtgatttaa
     4501 atcaattttt aaatattttc gctacgcaaa tattaactcg ttcgccactt gccgcggttt
     4561 gcccacgcct gaaggccagc aattacctcc gctttacaaa gatatgcttt tccatacaag
     4621 acctgatgct gccacccgag gcttaaccag ggtacaattg cccgctaatt aacacctgca
     4681 taaaactcaa ggagagtgca tgtctatcac ggcgcagtcc gtataccgtg acaccggaaa
     4741 tttcttccgt aatcaattta tgaccattct gttggtatcg ttgctatgtg cgtttatcac
     4801 agtggtgtta gggcatgttt tctcacccag tgatgcacag cttgcgcagc tcaatgacgg
     4861 cgtgcccgtt agcggcagta gtgggttgtt cgacctggtt cagaatatgt caccggaaca
     4921 gcaacaaatt ttgctgcagg cttcagcggc gtccactttt tcaggattaa tcggtaacgc
     4981 cattctcgcc ggaggcgtaa tattaattat ccagctggtg tctgcgggtc agagagtcag
     5041 tgcgctgcga gctattggtg ccagtgcgcc gatattgccc aagttattta ttctgatttt
     5101 tctgactacc cttttagtac agattggcat catgctggtg gtcgttccgg gaattatcat
     5161 ggccatttta ctggccctgg caccggtgat gctggtacag gacaaaatgg gcatttttgc
     5221 ctcgatgcgt agcagtatgc ggctgacttg ggcgaatatg cgtctggtgg cacccgcagt
     5281 actgagctgg ttgctggcaa aaacactgtt gctgcttttt gcctcttctt ttgccgcatt
     5341 aaccccggaa attggtgccg tactggcgaa caccttgagc aacctgattt ctgccatatt
     5401 gctcatctat ctgttccgcc tgtatatgtt gattcgccaa taaccttaag catttgatta
     5461 cggaatcgta aaatgaagca gtttcttgat tttttaccgc tggttgtctt tttcgcgttt
     5521 tacaagattt atgacatcta tgcggctact gcggcgctga tcgtcgccac ggcgattgtg
     5581 cttatctata gctgggttcg ctttcgtaag gttgagaaga tggccctgat cacttttgtt
     5641 ctggtggtcg tcttcggtgg cttgacgctg ttcttccaca atgatgagtt tataaaatgg
     5701 aaggttactg tcatttatgc cctgtttgcg ggtgccctgt tagtcagcca atgggtgatg
     5761 aaaaagccgc taattcagcg gatgctgggt aaagaactca cgctgccgca accggtatgg
     5821 tcgaagctga atctggcctg ggctgttttc tttatccttt gcggtctggc aaacatctac
     5881 atcgcattct ggctgccgca aaatatttgg gtcaacttta aagtctttgg cctgaccgcc
     5941 cttaccttaa tcttcacatt gttaagcggt atctatatct accgccacat gccgcaggaa
     6001 gataaatcct aaccagactg ccagtccttc ggggctggcg ctgtatctcc tctgttctcc
     6061 tcaggaaaat catagtagca tcgcgcctgt gattttcctt ttaagtcggt tttaccatgt
     6121 ctacaacaca taacgtccct cagggcgatc ttgttttacg tactttagcc atgcccgccg
     6181 ataccaatgc caatggtgac atctttggtg gttggttaat gtcacaaatg gatattggcg
     6241 gcgctattct ggcaaaagaa attgcccacg gtcgcgtagt gactgtgcgg gttgaaggaa
     6301 tgactttctt acggccggtt gcggtcggcg atgtggtgtg ctgctatgca cgctgtgtcc
     6361 agaaagggac gacatcggtc agcattaata ttgaagtgtg ggtgaaaaaa gtagcgtctg
     6421 aaccaatcgg gcaacgctat aaagcgacag aagcattatt taagtatgtc gcggttgatc
     6481 ctgaaggaaa acctcgcgcc ttacctgttg agtaatagtc aaaagcctcc ggtcggaggc
     6541 ttttgacttt ctgcttactg aatttcggtg gtgccgttaa ttttaaacag gatattcacc
     6601 acaatcccac tgcctggctt acccggctca taacgccatc tgcgcatcgc atttttcacc
     6661 tcacgctcaa acatgttcgc aggcttggct gagaggattt gtacgttatc cacgcgacca
     6721 tccggcgtga cgtcaaattt aactttaacc tgcccttcaa tgcgcaatgc ctgtgctcgt
     6781 gccggatact gcggctgatt acggcttaat gcgcgtggtc ctgaagccac actggtaacc
     6841 ggcttgctgg ttgcagccgt tgccgtactt gatgtggggc gcgcgggtgc cgtattttca
     6901 aacggtgatg ccggacgcga ctctacgggt ttgatatcgc gttttggctg ctcctgtacc
     6961 tttttcaccg gcttcggttt tggcttaggt ttcggcttcg gcttttcaat gaccaccggt
     7021 gcttcttttg gcggttcggg gatcggctca ggttccggtt ctggctctac caccggctcc
     7081 ggtggcggct gaacggcttg tggcggttcg agatcagcag gcgcaaccat cgtgacagaa
     7141 attggctgcg caggcgcagg tagttcaata acctgatgta ccgaggtata gagcagaccc
     7201 gccacaacag caccatgaat gcagaccgaa agtaacgtcg gccaggggaa gcggcgaggt
     7261 aaatcaaggg tcattgaagt cataatcatt tcagtagaaa aaccaggtct cgattttaaa
     7321 tgcaaatagc aatcatattc aataaggcaa gacgatcagg atcagtgaaa atgtcatttt
     7381 gggcgtaatt gaccttactt tatgcatggt cttaacaaag acatcatctt tccgttgcag
     7441 atcttcgccc tttcaaataa cgtactttac aactttcccg aacaaggagt tgtgcccgtg
     7501 ttgtatgtta tttacgctca agacaaagct gatagcttcg aaaaacgcct ttccgttcgt
     7561 ccggcacatt tagcacgttt acagttactg catgatgaag gtcgtttgtt gacagctggt
     7621 ccaatgccag cggtagacag taacgatcca ggggctgcgg gatttactgg ctcaaccgtt
     7681 attgctgaat ttgaatcgct ggaagcagct caggcctggg ccgatgctga cccgtacgta
     7741 gcggcaggag tctatgaaca cgtttcagtg aaaccattta agaaagtgtt ctgaatagag
     7801 attcaatatt taagggcacg gtttttgcaa ccgtgttctt gtttggcctt tccttatagc
     7861 cggtcagtgc attcaccacc acaagcagat aataacttta tctgtatttc tttatatgat
     7921 gtgaacatat tcaggaaaat gccctttcct gacgcagagt tgctcgctca aaacctttga
     7981 ttactgccga taaagtcatc tactattgtt taacacattc acgcgctgca tgtatgttca
     8041 gaaaatgagc acgctaagat aattaaacaa aattgtggtt acagaggtat cagtgagtca
     8101 ctgggctaca ttcaaacaaa cagcaacaaa tttatgggtg accctacggc acgacattct
     8161 cgcgctggcc gtctttttaa atggattgct tatttttaaa acaatctatg gtatgtcggt
     8221 caatttgctt gatattttcc atatcaaagc attttcagag ttggatctct ccttgctggc
     8281 aaacgcccca ctatttatgc tcggcgtctt tcttgtcctg aactccattg gcttactgtt
     8341 ccgggcaaag ctcgcatggg caatcagtat cattttgttg ttgatagcgc taatttacac
     8401 cctgcatttt tatccctggc tgaaatttag tattggattt tgcattttta cgctggtgtt
     8461 tttgctgata ctgcgcaaag acttctccca cagtagcgcc gcagccggga caatttttgc
     8521 atttattagt ttcacgacat tactgtttta ctcaacctac ggtgcgcttt atttaagcga
     8581 aggttttaat ccgcgaatag aaagtttgat gaccgcgttc tatttttcga tagaaaccat
     8641 gtcaaccgtc ggctatggcg atattgtccc tgtttctgaa tcagcacgat tgttcactat
     8701 ttcggtcatt atttccggca ttaccgtttt tgccacatct atgacctcaa tttttggtcc
     8761 gcttatccgc gggggattca acaaacttgt aaaaggaaac aatcatacaa tgcatcgtaa
     8821 agatcatttt attgtttgcg gacattcgat tctcgccatc aatacgatcc tgcaactgaa
     8881 tcaacgcgga caaaacgtaa cggttatcag caacttgcct gaagatgata tcaagcaact
     8941 tgagcaacgc ttaggcgata acgctgatgt tatccccggt gacagtaatg acagttcagt
     9001 attaaagaaa gcgggaatcg atcgttgccg ggccattctg gcgctgagtg ataacgatgc
     9061 agataacgcg tttgttgtac tctcggcaaa agatatgagc agtgatgtca aaacagttct
     9121 cgccgtcagt gatagcaaaa acctgaataa gattaagatg gtacatccgg atatcattct
     9181 ctcaccgcaa ctgtttggca gcgaaattct ggcgcgagtt ttaaatggtg aagagattaa
     9241 taatgatatg ctcgtttcaa tgttgttgaa ctccggtcat ggcattttca gcgataacga
     9301 tgaacaagaa acgaaagctg acagtaaaga atcagtgcaa aaatagagtt taaagacgaa
     9361 agagtaaact tattgggcac cgcaatggtg cccagtatat ttagatattg tagatcgcaa
     9421 acaataacga gttacgctgt ttgtttagaa tgcacttcct gatgctgtgg atacgcatat
     9481 ttcggcgcga ttgcccctca agccaacgcg atttacgtcg gctagcctga cgctgcatgc
     9541 gccagcgccc cacttccgtt ctactccgct tcatgttaac ta
//
LOCUS       DAZFQD010000176         9562 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.176, whole genome shotgun sequence.
ACCESSION   DAZFQD010000176 DAZFQD010000000
VERSION     DAZFQD010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9562
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.176"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..2898
                     /gene="evgS"
                     /locus_tag="ACHKTW_004583"
     CDS             <1..2898
                     /gene="evgS"
                     /locus_tag="ACHKTW_004583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HFO6465486.1"
                     /translation="YYNSPRQYNFFLTRKESVILNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPNLKVLENPYSPPYSMTDENGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLSPGGWDIIPGAIYSEDRENNVLFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIQVDNASAAFHKVKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     DSPFKDVFSNAHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASDNAVYI
                     CGWQDITETRDLINALEVEKNKAIKATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYATGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALSCSSTFPDHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLV
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTTFTITIPVEISQQVATVEAKAEHPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLSCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDI
                     EALKNNTANDLQLMQEILMTFQHETHKDLPAAFQALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPVSDDSKPEILQLLNSVKEHIAELDQEIAVFCQKND"
     gene            complement(2954..4099)
                     /gene="yfdE"
                     /locus_tag="ACHKTW_004584"
     CDS             complement(2954..4099)
                     /gene="yfdE"
                     /locus_tag="ACHKTW_004584"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HFO6465487.1"
                     /translation="MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLCNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     PGTMEKLGFSWETLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFNTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLARIHEVGVPVAPLLSVAEAIKLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(4173..5117)
                     /gene="yfdV"
                     /locus_tag="ACHKTW_004585"
     CDS             complement(4173..5117)
                     /gene="yfdV"
                     /locus_tag="ACHKTW_004585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HFO6465488.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFRGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFIVTAPLWIYVSRLVS"
     gene            complement(5187..6881)
                     /gene="oxc"
                     /locus_tag="ACHKTW_004586"
     CDS             complement(5187..6881)
                     /gene="oxc"
                     /locus_tag="ACHKTW_004586"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008949 - oxalyl-CoA decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HFO6465489.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPMIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESTQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(6935..8185)
                     /gene="frc"
                     /locus_tag="ACHKTW_004587"
     CDS             complement(6935..8185)
                     /gene="frc"
                     /locus_tag="ACHKTW_004587"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033608 - formyl-CoA transferase activity
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HFO6465490.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPEWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            complement(8698..9330)
                     /locus_tag="ACHKTW_004588"
     CDS             complement(8698..9330)
                     /locus_tag="ACHKTW_004588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HFO6465491.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQVQQTQKTAA
                     AERISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKT
                     NLNNDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVME
                     NQYLMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
BASE COUNT         2773 a         2171 c         1987 g         2631 t
ORIGIN      
        1 tattataact cgccgcgtca atataatttt ttcttgacca gaaaagaatc tgtcattctt
       61 aatgaagtac tcaatagatt tgttgatgct ttaacaaatg aagttcgcta tgaagtatca
      121 caaaattggc ttgatacagg aaacctggcc tttctgaaca aaccattaga actcactgaa
      181 catgaaaaac agtggattaa gcagcatccc aatttaaagg tgctggaaaa tccttactcg
      241 cccccctatt ctatgacgga tgaaaatggc tcggttcggg gcgttatggg ggacattctt
      301 aatattatta ccttgcaaac aggtttaaat ttttctccga tcaccgtttc acacaatatc
      361 catgctggaa cacagcttag ccccggagga tgggatataa tacctggcgc tatttatagt
      421 gaagatcgag aaaataatgt tttatttgct gaagccttca taacaacgcc ttacgttttt
      481 gtcatgcaaa aagcgcctga cagtgaacaa acattaaaaa aaggaatgaa agttgccatt
      541 ccatattatt atgagctgca ttcgcaatta aaagagatgt atccggaggt tgaatggata
      601 caggtcgata atgccagcgc tgcatttcac aaggttaagg aaggtgaact tgatgctctg
      661 gtcgcgacac agttaaattc gcgttacatg atcgatcatt actatcctaa tgaactttat
      721 cattttctta ttcctggcgt tccgaatgca tcgctttcgt tcgcttttcc tcgcggagaa
      781 ccggaactta aggatattat taataaagca ctgaatgcaa ttcccccaag cgaagttctg
      841 cgcctgacgg aaaaatggat taaaatgccc aatgtgacca ttgacacatg ggacctatat
      901 agcgagcaat tttatattgt tacgacatta tccgttttat tagttggcag tagcctttta
      961 tggggattct acctgttacg ctcagttcgt cgtcgtaaag tcattcaggg tgatttagaa
     1021 aaccaaatat cattccgaaa agcactctcg gattccttac cgaatccaac ttatgttgta
     1081 aactggcaag gtaatgtcat tagtcataat agtgcttttg aacattattt cactgcggat
     1141 tactacaaaa atgcaatgtt accattagaa aacagtgact caccctttaa agatgttttt
     1201 tctaatgcgc atgaagtcac agcagaaacg aaagaaaatc gaacaatata cacacaggta
     1261 tttgaaattg ataatggcat cgagaaaaga tgcattaatc actggcatac attatgcaat
     1321 cttcctgcaa gtgacaatgc agtatatatt tgtggttggc aagatattac tgaaacgcgt
     1381 gatctaatta atgcactcga ggtagaaaaa aataaagcga taaaggctac cgtagcaaaa
     1441 agtcagtttc tggcaacgat gagtcacgaa ataagaacac caataagctc tattatgggc
     1501 ttcctggaac ttctgtcggg ttctggtctt agcaaggagc aacgggtgga ggcgatttca
     1561 cttgcctacg ccaccggaca atcactcctc ggcttaattg gtgaaatcct tgatgtcgac
     1621 aaaattgaat cgggtaacta tcaacttcaa ccacaatggg tcgatatccc tactttagtc
     1681 cagaacactt gtcactcttt cggtgcgatt gctgcaagca aatccatcgc attaagttgc
     1741 agcagtacat ttcctgatca ttacctggtt aagatcgatc ctcaggcgtt taagcaggtc
     1801 ttatcaaatt tactgagcaa tgctctcaaa tttaccaccg agggggcagt aaaaattacg
     1861 acctccctgg ttcacattga tgacaaccac gctgttatca aaatgacgat tatggattct
     1921 ggaagtggat tatcgcagga agaacaacaa caactcttta aacgctacag ccaaacaagt
     1981 gcaggtcgcc agcaaacagg atctggctta ggcttaatga tctgcaaaga attaattaaa
     2041 aatatgcagg gcgatttgtc attagaaagt catccaggca taggaacaac atttacgatc
     2101 acaatcccgg tagaaattag ccagcaagtg gcgactgtcg aggcaaaagc agaacacccc
     2161 atcacactac ctgaaaagtt gagcatatta atcgcggatg atcatccgac caacaggcta
     2221 ttactcaaac gccagctaaa tctattagga tatgatgttg atgaagccac tgatggtgtg
     2281 caagcgctac acaaagtcag tatgcaacat tatgatctgc ttattactga cgttaatatg
     2341 ccgaatatgg atggttttga gttgactcgc aaactccgtg agcaaaattc ttccttaccc
     2401 atctgggggc ttacagccaa cgcacaggct aacgaacgtg aaaaagggtt aagttgcggc
     2461 atgaacttat gtttgttcaa accgttgacc ctggatgtac tgaaaacaca tttaagtcag
     2521 ttacaccagg ttgcgcatat tgcacctcag tatcgccacc ttgatatcga agccctgaaa
     2581 aataatacgg cgaacgatct acaactgatg caggagattc tcatgacttt ccagcatgaa
     2641 acgcataaag atctacccgc tgcgtttcaa gcactagaag ctggcgataa cagaactttc
     2701 catcagtgta ttcatcgcat ccacggtgcg gctaacatcc tgaatttgca aaagttgatt
     2761 aatattagcc atcagttaga aataacacct gtttcagatg acagtaagcc tgaaattctt
     2821 cagttgctga actctgtaaa agaacacatt gcagagctgg accaggagat tgctgttttc
     2881 tgtcagaaaa atgactaaat agcggctccc acaatgttca aatgtgggag ctatttacca
     2941 gcacatctta catttatgat gagaactcct ggcgaatttg ttccccatgc tggtcgagcg
     3001 ttgccgctcc cggcataaca tgcgggtccg cgcagccgct gatttttatc ggattacccg
     3061 gcatcattat tcccccggct tcaatcaaca tatttctcgc ctgagtttgt ggcaatttaa
     3121 tggcctcagc cacacttaat aacggcgcga cgggtacacc aacttcatgt attctggcta
     3181 accaaacttc agctgcctgc gtttttaacg tccgctcaat atattgttta agaatagcct
     3241 ggttttgtac gcgtaaaata ttgctgctaa atcggggatc attaaccagt tccgtaagct
     3301 ccagtgcctg gcataacgca gaaaaaagct tgtcattacc acaacaaatc gtaatcggct
     3361 tatcctgagt attgaaaaca tcaaaaggtg ccatgtaggg atggcgattt cccagacgtt
     3421 gtggtgactt ccctgtcgcg atatatgcca tcagaccatg ctccagaaaa ctcagcgtgg
     3481 catcaaacat cgctatatcg acatgcgccc ctctctggct cttttcgcgg ccataaagtg
     3541 cactcactat tccgctgaat aaatagacac cgccgcatag atccgcaaga gatgtaccaa
     3601 cacgcactgg cggagcatca ggatatcctg tttccatcat tatcccgctc attgcctgaa
     3661 tgatggtatc gtaggcagga gcatctttta gcggaccggt atgtccgaaa cctgacgatg
     3721 aagcatatat gaggcgcggg ttgatttctt gaagcgtttc ccatgaaaac cccagttttt
     3781 ccattgtacc tgggcgaaaa ttctcagcta atacatcagc ttgtttgagc atatttataa
     3841 atatactttt atcgtgatca ttctttaaat caagaaccac actctctttg ccatgattaa
     3901 taaaactgta atagagtgac tgtccatcca catagggacc aaatgtgcgg gtatcatcac
     3961 catgacccgg cggctcaact ttaattaccc ttgcgcccat attacaaaga agttgagttc
     4021 cgaaaggtcc attaaggaca tgtgtcatat cgataactaa taagccttca aacggccctt
     4081 tgctttcatt atttgtcatt tgcttatgcc ttgaaaaaag ccgaaggtga aaagacactt
     4141 cggcatttat gtaaaatcaa taacatgatt aattatgaaa ccagtcgact gacataaatc
     4201 cacaagggag ccgtgacgat aaagcccagt acgctcacag ccaatgacgc tgtaccagta
     4261 cgggtgtaga cattaaaccg gctggcaata atgattccgg agaatgccgg cggtaatgcg
     4321 cctgccagta ccatcatctg cagatgttcg ctgttcaaat gacatgccat accaacgaga
     4381 agcagtgcca gtggcatcag aatcagcttc aggaaggtgt tataagcaat ttcggcactg
     4441 aactcgaatt tatgtgcagc cagagtcaac cccgcagcga ataccgctac ccctgagtta
     4501 gccttcgcaa tcagattaaa ggttgggtcc catgctgccg gaatttttac cccaaccaac
     4561 accaggatcg ttgccagaac aggtgcccat actactggct cctttgccgc agaaattaat
     4621 gcgctcagat tactattctt cttaccatcc gctcctgagg aaggattcag caaatacaga
     4681 ccaataggaa tagtaattgc gttaacaata atagaaataa ttgctaccac taaacctgtt
     4741 gatacggaat caccataaat aggatcgaga actgcaaacc ccaggaatcc aatggtaggt
     4801 gaacccgcaa ttaatgcaca tacagctgct tctgcatggg tacgtttaaa aaatttgtag
     4861 caaccaaacc aggagaaaaa gaaacatccg acaataacca caagtgatac aagggtcaga
     4921 cgagtgtccg caaaaatcat ttccctgttt gcccgggtaa tagaaacaaa tagagccgca
     4981 ggaagagcgt agtttaatac cagtttatta aatgcgcggg cttgatcttc tgaaaaagtt
     5041 tctcgtctgc ctctaaaata acccaacagc atgattacga taataggcaa taaatcgcca
     5101 ataaaaaatg ttagcataat agtatttccg ataagggtcc cctcaccatc gcataatgag
     5161 ttggcaagag gagacgatgt cagtatttaa ttaccagcga cttgttttgg gttaagtttg
     5221 gtaatatggc cactttcagt tcctgctgca gggtcgatga ccacattaat aatggtcggt
     5281 ttgcgcgact gaataccggt ggttaaagca tgacgaagtt catctgtcgt ggtgacgtta
     5341 tagccaacgc cacgaaacgc atccattaat ttgtcatacc ttgcatggtg caacagatcc
     5401 gttggtgatg gtgcaccagc gccactgaga tcaacaccgt ctcctctgta gatgccgcca
     5461 ttattaaaaa taacgatcgt caccggcagg ttatatcgac aaatcgtttc aatttccatc
     5521 ccactgaaac caaaagcact atcaccttca atggcgacaa ccggagaacc agaagtcacg
     5581 ctagcaccga tggcatagcc cataccgatg cccatgacac cccaggtacc acaatccaga
     5641 cgacgacgtg gtttatacat atcaataata tttcgtgcat tatccagggt atttgcacct
     5701 tcattaacta aataaatatc ctggttctcg cgcaatacgt cgcgcacagc acttaatgca
     5761 ttaaagtaat ttaatggttg agtatctgta cttaattttt catgcatttt ttgtgcattt
     5821 tgctgcttgt ggatatttaa aatatcgcgc cataccagtg gagtcgtaaa tgtgttttgt
     5881 ttcagttctg ccagcatacc ttgcatactg gatgcaatat caccaacgac tggcacagca
     5941 atggggcggt tgctgtcaat ttcctgcggt tcaatatcca gttgaataaa ctgtgtatct
     6001 gccgcccatc cttttttacc gtgtgccaat aaccaattca gtcgtgcacc aacaagcatg
     6061 acaacgtcag catttgccag ggcaaacgaa cgcgcagctg ccgcagaaag tggatgcgta
     6121 tcttcaagga tccctttcgc catagacatt ggcaggaatg gaatctgagt actttcaata
     6181 aattcacgaa gctgttcatc agcttgtgaa tacgccgcgc ctttgccaag gataattaat
     6241 ggccgttcag cttttgctaa aagcgaaatt gcgctagtga ctgacttcgg gcatggcaat
     6301 aatgctggcg atggattttc aactttaaca atcgtggtta acgcttcgtc tttttccatc
     6361 gtcgcggcca ggacatttgc tggcaaatca agataaactc cgccagggcg acccgataca
     6421 gagacccgga tagcgcgtgc caatgcaatg ccaagatcct gcggctgatt aacgcgaaat
     6481 gctgctttgg catacggttt tgccgcattc atttggtcca gctcttcata atcaccttgc
     6541 tgtaggtcga cgatcgcgcg gtcgctggag ccgctaatca tgatcatcgg aaaaccattt
     6601 accgttgcgt tggccaatgc ggtcaaacca ttgaggaatc ctggcgcaga aactgtcagg
     6661 cagatccccg gtttttgggt aagaaaaccg cttgccgcag cggcatagcc tgccgactgc
     6721 tcatgacgaa aaccaatata acgaatgcct tccgcctggg catggcgtgc catatccgtc
     6781 acaggaatac ctacaacacc ataaatagtg tcaatattat tctgttttaa tgcttcaacg
     6841 atgatatgca taccatctgt catttgaagt tgatctgaca tgattaataa cctcgaaatt
     6901 tttataaatt gggagccaga agcccccgtt aatatcagat ggcgtggttt tgcttcattg
     6961 cagcaatttc atcgtcgcta taacccagct cctgcaatac agcagcggta tgttcaccta
     7021 atagcggcgc agctttaata tccggcgtaa aggcagagaa tttcattgga caaccaacgg
     7081 tcagatattt tccacgcaac ggttgttcca cttcaacaac actgccactt tggcgcaaag
     7141 agggatcaag tgaaatttct ttcatactta aaaccggtgc acaaggaata tcaaactgag
     7201 tcaaataggc caccgcttca tgtttatcaa tagtgacagt gtatttttcg atttcagcaa
     7261 aaatatcgaa aatatgtggc tgtcgggcat gggctgtact gtatgccgga tcggtaatcc
     7321 attctggttt gccgatggct ttacaggtgt tttcccagtt ttgctcctga atagtgaaat
     7381 aaatataggc gttaggatcg gtttcccagc ctttacattt caggatccag ccaggctgac
     7441 cgccaccacc tgcattacca ccgcggggaa ctgcatcacc aaatgtacca ttcggatact
     7501 gcgggtattc ttccagataa cccaatttat cgagacgctg ctggtcacgt aatttcacgc
     7561 ggcaaaggtt caatacggca tcctgcattg acatggtgac tcgttgccca cgccccgttt
     7621 tttcgcgatg cagcaaagca gcaagtaaac cgatcagcaa atgcattccg gtgttgctgt
     7681 cacccaacgc tgcagcgctt accagcggcg gaccatccca aaaacccgta gtggatgccg
     7741 cgccacccgc tgcctgagca acgttttcat aggcttttac attcacataa ggcgaacact
     7801 catcaaaccc tttgatcgaa ccaaaaatca gacgtggatt gatttcttga atatgctccc
     7861 aggtgaagcc catgtgatca atggcccctg gatgaaagtt ctcgactaag atatcagctt
     7921 cgcggatcag cttttccatt acctctttgc cttccgctgt tttggtattt aactcaatag
     7981 aacgtttgtt actgttaagc atggtgaagt aaagcgcatc gatatcagga atatctcgca
     8041 gctggtgacg cgttacgtca ccaacgccgg gacgttcaat tttaataacg tcagcgccaa
     8101 accaggccag catttgagta caagatgggc cagattgcac accggtgaaa tcgagaactt
     8161 taattccttg aagtggagtt gacatagcaa tacctattag ttaattaaat atgtgttaat
     8221 ttagcgttta tttaaggcgt aatatcttct ataaataacc cgcaaaatat cgtcgtagct
     8281 gcactcgata tttttaaaat cctgtatcaa gtgctgaaaa tcatttcatc gccacttcca
     8341 attcctttct gcatggtaag tagtgaggac gaaaagaaga atatgataaa tattgattcg
     8401 tggtaatcag agagagtcgc gacatttgtg atgttattct tacaactgta agaagcgccg
     8461 gtgatttaca agtaagggta tgattacgct ttttaaagcg taccataaaa cataacataa
     8521 taaatgattt gtgatgacac gggattattg ggatttactt tgaggaagat aaaatgcgtg
     8581 tttatagaat cgcgagcgcc aaataatttt atgaccacaa cctgtcgtat tgtggtcata
     8641 aaaaattgtc ggctggaggg agttaaatcc tttacctcaa tatactgcct gtcagcatta
     8701 gttcacaaac agtgcttcgc tgtcgacgat gattccatct tccgcacctt taagcgccag
     8761 gtttgcctcg tagtattgct ttttatcaag cagtttctgc gcatcagcaa gtgcattacg
     8821 agtctgttta agcggcatca gatattggtt ttccattacg ccaacaccgg ccaggcgaag
     8881 ttcttccatt gcgccctttt tatccccttt cgccattttt tcattggcaa tttttatcgc
     8941 ggcttctttt tcgggagttg ctacataact ctcagaaatt ccgacagatg cattaatgac
     9001 aatatattgg tcattattga gattggtctt tttacctggc ttagcgaact ttgcccattc
     9061 ggtactgtca tctgacagca aagcggaagc ttcattggtc agttcttttg ccttttctgg
     9121 atcgccatga aacagagcca ggcgcgctac ctgcacgtca cgcatcgcat ataaaccttg
     9181 ttcagagata cgctctgctg ctgccgtttt ctgggtttgc tgaacttgct gagtttgctg
     9241 ttgtgccgct accggtgcat tatcagcagc ccatacagtt gaagatgcca gaattgctgt
     9301 gaccatcgta gccataatta aacgtttcat acttacctca ttctttaaca gttttagaaa
     9361 acgccgccat catattgggt gagcatgaaa tgagaaatca gagagcaacg gtcattttga
     9421 aagggttatc ttacagttgt agttattatc gcaagaagta ttttagtctt acaattgtag
     9481 gaatctgctt cctgtcgcag agaactattc ttatgtacca caaagagatg tcatttatta
     9541 tatcgatgta aatattgatt aa
//
LOCUS       DAZFQD010000177         9559 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.177, whole genome shotgun sequence.
ACCESSION   DAZFQD010000177 DAZFQD010000000
VERSION     DAZFQD010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9559
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.177"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(46..219)
                     /locus_tag="ACHKTW_004589"
                     /pseudo
     CDS             complement(46..219)
                     /locus_tag="ACHKTW_004589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052655.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            289..495
                     /locus_tag="ACHKTW_004590"
     CDS             289..495
                     /locus_tag="ACHKTW_004590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000547968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFO6465492.1"
                     /translation="MYIPQNFLPKSRQKLLRSAPGQSFPSVAVMPLAAKVLKKVMVNT
                     GGNKRQVPAKRQNRGSRNSKDDGG"
     gene            521..1060
                     /locus_tag="ACHKTW_004591"
     CDS             521..1060
                     /locus_tag="ACHKTW_004591"
                     /inference="COORDINATES: protein motif:HMM:NF010292.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFO6465493.1"
                     /translation="MAVRGINKVILVGRLGKDPEVRYIPNGGAVANLQVATSETWRDK
                     QTGEMREQTEWHRVVLFGKLAEVAGEYLRKGAQVYIEGQLRTRSWEDNGITRYVTEIL
                     VKTTGTMQMLGRAAGAQTQPEEGQQFSAQPQPEPQSEAGTKKGGAKTKGRGRKAAQPE
                     PQLQPPEGDDYGFSDDIPF"
     gene            1123..1356
                     /locus_tag="ACHKTW_004592"
     CDS             1123..1356
                     /locus_tag="ACHKTW_004592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HFO6465494.1"
                     /translation="MSEYFRILQGLPDGPFTRKHAEAVAAQYRNVFIEDDHGEQFRLV
                     VRNNGAMVWRTWNFEDGAGYWMNHVIRDFGILK"
     gene            1422..3380
                     /locus_tag="ACHKTW_004593"
     CDS             1422..3380
                     /locus_tag="ACHKTW_004593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFO6465495.1"
                     /translation="MSVTESKAKTERKSSRKPAKTQETVLSALLAQTEEVSVPLASLI
                     KSPLNVRTVPYSAESVSELADSIKGVGLLQNLVVHALPGDRHGVAAGGRRLAALNMLA
                     ERNILPADWPVRVKVIPQELATAASMTENGHRRDMHPAEQIAGFRAMAQEGKTPAQIG
                     DLLGYSPRHVQRMLKLADLAPVILDALAEDRITTEHCQALALENDTARQVQVFEAACQ
                     SGWGGKPEVQTIRRLVTESEVAVAGNTKFRFVGADAFSPDELRTDLFSDDEGGYVDCV
                     ALDAALLEKLQAVAEYLREAEGWGWCAGRMEAVGECREDARAYRNLPQPEAVLTEAEE
                     ERLNELMARYDALENQCEESDLLEAEMKLMRCMAKVRAWTPEMRAGSGVVVSWRYGNV
                     CVQRGVQLRSEDDVADDADHTEQMQEKASVEEISLPLLTKMSSERTLAVQAALMQQPD
                     KSLALLAWTLCLNVFGSGAYSKPAQISLECKHYSLTSDAPSGKEGAAFMALMAEKARL
                     AALLPEGWTRDMTMFLSLSLEVLLSLLSFCTACSLNGVQTRECGHTSRSPLDSLETAI
                     GFHMRDWWQPTKANFFGHLKKPQIIAALNEAGLSGAARDAEKMKKGDAAEHAEHHMKD
                     NRWVPGWMCAPHPQTDTTERTDNLADAA"
     gene            3435..3869
                     /gene="psiB"
                     /locus_tag="ACHKTW_004594"
     CDS             3435..3869
                     /gene="psiB"
                     /locus_tag="ACHKTW_004594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000845953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugation system SOS inhibitor PsiB"
                     /protein_id="HFO6465496.1"
                     /translation="MKTELTLNVLQTMSAQEYEDIRAAGSDERRELTHAVMRELDAPD
                     NWTMNGEYGSEFGGFFPVQVRFTPAHERFHLALCSPGDVSQVWVLVLVNAGGEPFAVV
                     QVQRRFAPEAVSHSLALAASLDAQGYSVSDIIHILMAEGGQA"
     gene            3866..4628
                     /locus_tag="ACHKTW_004595"
                     /pseudo
     CDS             3866..4628
                     /locus_tag="ACHKTW_004595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052659.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
     gene            complement(4597..4785)
                     /locus_tag="ACHKTW_004596"
     CDS             complement(4597..4785)
                     /locus_tag="ACHKTW_004596"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6465497.1"
                     /translation="MKINLLRGYLLSATQHGNKPPSRHEAESLKRRAHHSPYTCTLTT
                     LTFPENNPLIQTVHGKSV"
     gene            4807..4956
                     /gene="mok"
                     /locus_tag="ACHKTW_004597"
                     /pseudo
     CDS             4807..4956
                     /gene="mok"
                     /locus_tag="ACHKTW_004597"
                     /inference="COORDINATES: protein motif:HMM:NF010277.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid maintenance protein Mok"
     gene            4898..5023
                     /locus_tag="ACHKTW_004598"
     CDS             4898..5023
                     /locus_tag="ACHKTW_004598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HFO6465498.1"
                     /translation="MLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK"
     gene            5243..5473
                     /locus_tag="ACHKTW_004599"
     CDS             5243..5473
                     /locus_tag="ACHKTW_004599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001426396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6465499.1"
                     /translation="MACQKSHHLPCDYSLFLPALILTTAVLSCLPGAPSAPGCLAASG
                     RQARLRQAAAGAPVSLRQTRWRGFGLSLRSDA"
     gene            complement(5493..5630)
                     /locus_tag="ACHKTW_004600"
                     /pseudo
     CDS             complement(5493..5630)
                     /locus_tag="ACHKTW_004600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052655.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5700..5906
                     /locus_tag="ACHKTW_004601"
     CDS             5700..5906
                     /locus_tag="ACHKTW_004601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000547968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFO6465500.1"
                     /translation="MHIPQKFLPKSRQKLLRSAPGQSFPAVAVMPLAAKVLKQVMVNT
                     GGNKRQVPAKRQNRGSRNSTDDGG"
     gene            5931..6218
                     /locus_tag="ACHKTW_004602"
     CDS             5931..6218
                     /locus_tag="ACHKTW_004602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001825190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6465501.1"
                     /translation="MSTRNIHVNTASYTLLVAGKKKNTGEEWDVLEFSSLTELKKYRK
                     SHPEKMAFSYSYALSRGVDTQFRHINIAEADHFKQFLRQIKRAGLDIRAIC"
     gene            6337..7158
                     /locus_tag="ACHKTW_004603"
                     /pseudo
     CDS             6337..7158
                     /locus_tag="ACHKTW_004603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234456.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
     gene            complement(7454..8044)
                     /locus_tag="ACHKTW_004604"
     CDS             complement(7454..8044)
                     /locus_tag="ACHKTW_004604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020246007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HFO6465502.1"
                     /translation="MVRTTNLLSLFVEWVKLFTDKVTRGGKMKKWMLAICLMFINEIC
                     QATDCFDLAGRDYKIDPDLLRAISWKESRYRVNAIGINPVTGYGSGLMQVDSQHFNEL
                     ARYGIKPEHLTTDPCMNIYTGAYYLAIAFKKWGVSWEAVGAYNAGFRKTERQNQRRLA
                     YASDVYRIYTGIKSSKGIRIPATKKSLPEINSVQNN"
     gene            8396..8779
                     /gene="traM"
                     /locus_tag="ACHKTW_004605"
     CDS             8396..8779
                     /gene="traM"
                     /locus_tag="ACHKTW_004605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer relaxosome DNA-binding protein
                     TraM"
                     /protein_id="HFO6465503.1"
                     /translation="MAKIQVYVNDHVSEKINAIAVQRRAEGAKEKDVSYSSIASMLLE
                     LGLRVYEAQMERKESAFNQAEFNKLLLECVVKTQSTVAKILGIESLSPHVSGKPKFEY
                     ANMVEDIREKVSVEMERFFPKNDDE"
     gene            8970..>9559
                     /gene="traJ"
                     /locus_tag="ACHKTW_004606"
     CDS             8970..>9559
                     /gene="traJ"
                     /locus_tag="ACHKTW_004606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000332518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer transcriptional regulator
                     TraJ"
                     /protein_id="HFO6465504.1"
                     /translation="MCAMDRRERALISQLHFPHSLGEAISFLKYPVCVRFEDGSFIQE
                     NNRFEKLIRSSFNSCDEWFDSLTLECKLQLSRAEIESCSSVYGVNCNNDILLNNMLWS
                     VIIESVITPCGYFFIWRFIWVANDNLSSFVVSKYTNELIVLSDEYVGIEPYLIGFSHH
                     YSSAKMNITVSKSKKKTMKLFKRHGFSSRDLWLDEM"
BASE COUNT         2349 a         2264 c         2664 g         2282 t
ORIGIN      
        1 cgaacccgct ggcgcggttt cgggctgtcg ctacggtccg atgcgtgaaa accccgggct
       61 tgcagcgacc gttccggtcg cgccgcctct gtcgcgccgt ggcctgcgct cctggcatcc
      121 cggcaaaagg ccgccgggat gtaaggggca acgccgtttc gcaaaccgcg cttcacgtcg
      181 ttgcggcggg tccctgcccg ttgttctgtc cggtgccatt cacctgttta agcccctgtc
      241 gccgggggca aagacagcca caaccgtctc cgccagtcca ggggtgaaat gtacatcccg
      301 caaaactttt tgcccaaaag caggcaaaaa cttttgcggt ctgcccccgg acagtcgttt
      361 ccgtccgtgg cagtgatgcc ccttgcggcg aaggttctca aaaaggtgat ggtgaatacc
      421 ggagggaaca aaaggcaggt ccccgcaaaa cgtcaaaacc gcggctcccg aaactcaaaa
      481 gatgatgggg gctgaaaaca tcaacagaag gaggcacatc atggcagttc gtggcattaa
      541 caaggtcatt ctcgtaggac gcctgggaaa agatccggaa gtccgttaca tccccaacgg
      601 gggcgcagtg gcaaacctgc aggtcgccac gtcggaaacc tggcgtgaca aacagacagg
      661 ggagatgcgg gagcagacag aatggcatcg cgtggtgctg ttcggcaagc tcgcggaagt
      721 ggctggcgaa tatctgcgca agggcgcgca ggtctacatc gaaggtcagc ttcgcacccg
      781 tagctgggaa gataacggta tcacccgtta cgtcaccgaa attcttgtta agaccacggg
      841 caccatgcag atgctgggac gtgccgcagg tgctcagact cagccggaag aggggcaaca
      901 gttcagcgct cagcctcagc cggaaccaca gtcggaggcc ggtacgaaaa aaggtggcgc
      961 aaaaacgaaa ggccgtggac gtaaggccgc gcagccggag cctcagctgc aaccgccgga
     1021 gggtgacgat tacgggtttt cagacgatat cccgttctga tcgggctgac tgtgataacc
     1081 gccccgcccc gttctgtgcg gggcatcacc ggagagatga gaatgagcga atatttcaga
     1141 atacttcagg ggctgcctga cggtcctttt acccgcaaac atgccgaagc tgttgccgca
     1201 cagtaccgga acgtctttat cgaggatgac cacggcgaac agtttcgcct ggttgtccgt
     1261 aataacggtg caatggtctg gcgtacctgg aattttgagg acggtgccgg gtactggatg
     1321 aaccatgtca tcagggattt cgggattctt aagtaagaga ggtgccggac ggggacacgt
     1381 ccggcagaca atcattatta ccgttttggg aggtactaac tatgtcagtt acagagtcta
     1441 aggcaaaaac ggagcgtaaa tccagccgta aacctgcaaa aacgcaggaa acagtcctgt
     1501 ctgccctgct ggcgcagacg gaggaagtga gcgtgccgct ggcctcactg attaaatcac
     1561 cgctgaatgt gcgcacggtg ccgtattctg cggagtccgt cagcgaactg gcggactcca
     1621 ttaaaggtgt cggcctgtta cagaatctgg ttgttcatgc cctgccaggt gaccgccacg
     1681 gtgtcgccgc aggtggtcgc cgactggcag cactcaacat gctggcagag cgcaacatcc
     1741 ttccggcgga ctggcctgtc cgtgtgaagg tcattccgca ggaactggcg actgccgcat
     1801 caatgactga aaacggtcat cgtcgggata tgcaccctgc cgaacagatt gccggattcc
     1861 gcgcaatggc gcaggaaggc aaaacacctg cacaaatcgg tgatttgctg ggctattcac
     1921 cccgccacgt tcagcgaatg ctgaaactgg ctgaccttgc gcctgtcatc ctcgatgcgc
     1981 tggcagaaga ccgcatcacc acagagcact gtcaggcgct ggcgctggag aacgacaccg
     2041 cgcgtcaggt gcaggtgttt gaagctgcct gtcagtcggg atggggcgga aaaccggaag
     2101 tacagaccat tcgtcgtctg gtgaccgaaa gtgaagtggc ggtggcgggg aacactaaat
     2161 tccgcttcgt gggggctgat gccttctcgc cagatgaact gcgcactgat ttgttcagcg
     2221 acgatgaggg tggctatgtg gactgtgttg cgctcgatgc cgccctgctg gaaaaactcc
     2281 aggctgttgc agagtacctt cgggaagccg agggctgggg atggtgtgcc ggacgcatgg
     2341 aggccgttgg tgagtgccgt gaagatgccc gggcataccg caacctgccg cagccggaag
     2401 cagtgctgac ggaggcggaa gaagaacgcc tgaacgaact gatggcacgt tacgacgcac
     2461 tggaaaacca gtgtgaggaa tccgacctgc tggaagcaga aatgaagctg atgcgctgca
     2521 tggcgaaggt cagagcgtgg acgccggaga tgcgtgccgg aagcggtgtg gtggtgtcct
     2581 ggcgttatgg caacgtgtgt gtccagcgtg gtgtgcagtt gcgcagtgag gatgacgtgg
     2641 ctgacgacgc tgaccacacg gaacagatgc aggagaaagc gtcagtggag gaaatcagtc
     2701 tgccactgct gacgaaaatg tcttccgaac gcacgctggc agtccaggcg gcactcatgc
     2761 agcaaccgga caaatctctg gcactgctgg catggacgct ctgcctgaat gtgtttggca
     2821 gcggagcgta cagtaaacca gcacaaatca gcctggaatg taaacattat tcgctgacca
     2881 gcgatgcgcc gtcagggaag gaaggtgccg cattcatggc gctgatggca gaaaaagccc
     2941 ggcttgcagc cctgctgccg gagggatgga cacgggacat gacgatgttc ctgtcactca
     3001 gcctggaggt gctgttatcc ctgctcagtt tctgtaccgc gtgcagtctt aacggtgtcc
     3061 agacccgcga gtgtggtcac acgtcacgca gtccgcttga ctcgctggaa accgctatcg
     3121 gattccacat gcgcgactgg tggcagccga caaaagcaaa cttcttcgga cacctgaaaa
     3181 agccgcagat tatcgcagcc ctgaatgagg caggactgtc cggtgctgca cgggatgcag
     3241 agaagatgaa gaaaggtgat gcggctgaac atgcagagca ccatatgaaa gacaaccgct
     3301 gggtgcctgg ctggatgtgt gcaccacatc cacagacgga taccactgaa cgcaccgata
     3361 acctggctga tgccgcctga taaacaacca caccgccccg ccggagacgg ggcggcagca
     3421 agggagatac cgtgatgaaa actgaactga ccctgaatgt attacagacc atgagcgcac
     3481 aggaatatga agatatccgg gcagccggaa gtgatgaacg tcgtgagctg acacacgccg
     3541 tgatgcggga actggatgcg ccggacaact ggacgatgaa cggcgagtac ggcagcgagt
     3601 ttggcggatt tttccccgtc caggtccgtt tcacgcctgc ccacgaacgt ttccacctgg
     3661 cgttatgttc accgggcgat gtctctcagg tctgggtgct ggttctggtg aatgccggtg
     3721 gtgagccgtt tgcggtggtt caggtacagc ggcggtttgc accggaagcc gtcagccatt
     3781 cactggcact ggcggcgtca ctggacgcgc aggggtacag cgtcagtgac atcatccata
     3841 tcctgatggc ggaaggaggt caggcatgag cgcacgttca caggcactgg ttcctctcag
     3901 cacagagcaa caggccgcat ggcgggcggt ggcggagacc gaaaaacgcc gccatcaggg
     3961 taacacgctg gctgaatatc cgtatgcggg ggcgtttttc cgctgtctga acggaagccg
     4021 caggatatcg ctgtctgacc tgcgattttt catgccgtcc ctgaccgcag aagaactgca
     4081 cggcaaccgt cttcagtggc tgtatgccat cgatgtgctg attgaaacac agggcgaggt
     4141 ctgcctcctt ccccttcccg gtgatgcagc agagcgcttg ttcccgtcgg tgcggtttcg
     4201 tgtcagggaa cgcagtcgcc ataaatccgc gctggtcatg cagaagtaca gccgccagca
     4261 ggcgagggag gcagagcaga aagcccgtgc gtaccaggcg ctggtcgctc aggctgaaat
     4321 cgaactggca ttccattcgc cggaaacagt cggaagctgg catgcacgct ggagtgaccg
     4381 ggtggcagag catgacctgg aaaccctgtt ctggcagtgg ggcgagcgtt ttccgtcact
     4441 ggccggaatg gaacgctggc agtggcagga tatgccattc tggcaggtca tcgcagaggc
     4501 tggtatggcg gcaagggtgg tcggtcatgc tgttcgtgag atggagcggt ggatggtgcc
     4561 gaacaaactc cgggaggcag cgtgatgcgg caacaatcac acggatttcc cgtgaacggt
     4621 ctgaatgagt ggattatttt cagggaaagt gagtgtggtc agcgtgcagg tatatgggct
     4681 atgatgtgcc cggcgcttga ggctttctgc ctcatgacgt gaaggtggtt tgttgccgtg
     4741 ttgtgtggca gaaagaagat agccccgtag taagttaatt ttcattaacc accacgaggc
     4801 atccctatgt ctagtccaca tcaggatagc ctcttaccgc gctttgcgca aggagaagaa
     4861 ggccatgaaa ctaccacgaa gttcccttgt ctggtgtgtg ttgatcgtgt gtctcacact
     4921 gttgatattc acttatctga cacgaaaatc gctgtgcgag attcgttaca gagacggaca
     4981 cagggaggtg gcggctttca tggcttacga atccggtaag tagcaacctg gaggcgggcg
     5041 caggcccgcc ttttcaggac tgatgctggt ctgactactg aagcgccttt ataaaggggc
     5101 tgctggttcg ccggtagccc ctttctcctt gctgatgttg tacgggcatg aacaacctga
     5161 cttcaggaag gtcgtttccc ttcaggaacg gggatgaacg tgcgcctgcg gcgcccgttc
     5221 ttttcccccg ccttctctgg ttatggcctg tcagaaatca catcaccttc cctgtgatta
     5281 ttctcttttt cttcctgctc tgattctgac tactgcagtg ttgtcctgtc tgccgggcgc
     5341 accttcggct cccggctgcc tggcggcatc cggtcgtcag gcccgactcc gacaggcagc
     5401 tgctggcgca cctgtcagtc tccgccaaac ccgctggcgc ggtttcgggc tgtcgctacg
     5461 gtccgatgcg tgaaaacccc gggcttgcag cgaccgttcc ggtcgcgccg tggcctgcgc
     5521 tccttacatc ccggcaaaag accgccggga tgtaaggggc aactccgttt cgcaaaccgc
     5581 gcttcacgtc gttgcggcgg gtccctgccc gttgttgtgt ccggtgccat tcacctgttt
     5641 aagcccctgt cgccgggggc aaagacagcc acaaccgtct ccgccagtcc aggggtgaaa
     5701 tgcacatccc gcaaaagttt ttgcccaaaa gcaggcaaaa acttttgcgg tctgcccccg
     5761 gacagtcgtt tccggccgtg gcagtgatgc cccttgcggc gaaggttctc aaacaggtga
     5821 tggtgaatac cggagggaac aaaaggcagg tccccgcaaa acgtcaaaac cgcggctccc
     5881 gaaactcaac agatgatggg ggctgaaaac cagagagagg agaaagcagg atgagtaccc
     5941 gaaatatcca cgttaacacc gcgtcgtaca cgcttcttgt tgccgggaag aaaaaaaaca
     6001 ccggggaaga gtgggacgtc ctggaattca gcagcctgac tgagctgaaa aaatatcgca
     6061 aaagccaccc ggaaaagatg gccttcagct atagctacgc gctcagccgg ggtgtggata
     6121 cgcagttccg ccatatcaac attgcggaag ccgatcactt caaacagttc ctgcgtcaga
     6181 ttaagcgtgc cggcctcgat atccgggcta tctgctgacc ggatgtgatg aatacccccc
     6241 ctgattgttg caatcttgtt aatggagatc gggagggatg atattaagcg gaaaaatttt
     6301 ttattaaatt tattctttca taaaggagta gctgttatgc gattagcttc ccgttttggt
     6361 cggtataatt ccatccgccg tgaacgtcct ttaacggatg atgaattaat gcagttcgtg
     6421 ccttcggtat tttccggtga taaacatgag tcccggagtg aacgttatac gtatattcca
     6481 acaatcaata tcatcaataa gttacgtgat gaaggtttcc agccattctt tgcctgtcag
     6541 agtcgggttc gtgatttggg acgtcgcgaa tacagtaaac atatgttacg tcttcgcagg
     6601 gaagggcata ttaacgggca ggaagttcct gaaattatcc tgcttaattc acatgatggt
     6661 tcatccagtt atcagatgaa ccccggaatt tttcgttttg tctgcacaaa tggcctggtg
     6721 tgcggaaata attttggcga aatccgcgtt ccacataaag gtgatattgt cgggcaggtt
     6781 atcgagggtg cgtatgaagt actcggtgtc tttgataagg tcactgataa tatggaggca
     6841 atgaaagaaa ttcatcttaa cagtgacgag caacatttat ttggcagagc tgcactgatg
     6901 gtcaggtatg aagatgaaaa taaaacgcca gtgacgcctg aataaataat tactccccgt
     6961 cgtcgggagg ataaacagaa cgatctctgg acaacctggc agcgggttca ggagaatatg
     7021 ataaaaggtg gattatctgg gcgaagtgcc tccgggaaaa ataccaggac aagagtcatt
     7081 acaggtattg atggtgatat aagaatcaac aaggcgttat gggtgattgc cgaacagttc
     7141 agaaagtgga agtcatgata atataagtaa agccccgaaa atttttcggg gctttactta
     7201 tattatcatt cttcggcatc gctgtgtcgt ctgatatacc tcaatttcat caactggctt
     7261 tccggttgtc tgtaaatatt gtgcccgcga aaaatggtct ggcaggaggc tgcgcagtta
     7321 ccgggtatat ataagatggg ttcccgcttc gcggggcttc ggccagtcag tattgtcact
     7381 catatgatat ttttgtgtgt ggccttccat gccgcttttg cggcataaca ggtatctgac
     7441 aatgtctgta agattaattg ttctgcacgc tgttaatttc cggaagtgat ttcttcgtgg
     7501 ccggaatccg gataccttta ctgcttttta tcccggtata aatccggtaa acatctgatg
     7561 cgtaggcaag acgtctctgg ttctggcgtt cggtcttcct gaatccggca ttgtatgcac
     7621 caacggcctc ccaggagacg ccccattttt taaatgctat tgccagataa taagcaccgg
     7681 tataaatgtt catgcaggga tctgttgtca gatgttctgg cttaattccg tagcgggcca
     7741 gttcgttaaa atgctgggaa tctacctgca tcagtccgct gccatatccc gttaccggat
     7801 taataccgat ggcattaacc cggtaacggg attctttcca tgatattgct ctcagtaaat
     7861 caggatctat tttgtaatcc cggcctgcaa gatcaaagca atcagtggcc tggcagatct
     7921 catttataaa catcaggcag atggctaaca tccacttttt catttttcca cctctggtga
     7981 ctttatccgt aaataattta acccactcca caaaaaggct caacaggttg gtggttctca
     8041 ccaccaaaag caccacaccc cacgcaaaaa caagtttttg ctgatttgtg ttttaaatca
     8101 tgtaattaat gaactatatt tatattatat aattttgaat tatattgcag agattcgtga
     8161 tagattcgat agtgaatcta tcacgaattg tcaatgattc agtaatgact attttttttg
     8221 aaaacatcta tttacttggc gcgtgaatct atattagatt cgtcatttga tgattgctga
     8281 ttcgtatagt gaatcgtgat tttagtatga tgaatctaat ggtgattcgt cataaggtgc
     8341 agaatagaga gcacgaaaat atattgtttg tttaggaagg tgaataaggt gtgttatggc
     8401 caaaattcag gtttatgtta atgaccatgt gtctgaaaaa ataaatgcta ttgctgtgca
     8461 aaggcgagca gagggagcaa aagagaaaga tgttagttat tcaagtattg cctctatgtt
     8521 gcttgagtta ggattgcgag tttatgaagc gcagatggaa cgaaaagaat cagcgtttaa
     8581 tcaggcagag tttaataaac ttcttcttga gtgtgttgta aaaacgcagt caacggtggc
     8641 aaagatttta ggtattgagt ctcttagccc tcatgtttcc ggaaaaccga agtttgaata
     8701 tgccaatatg gttgaagata tcagagagaa ggtgtcggtt gagatggaaa ggttctttcc
     8761 aaaaaatgat gatgaataaa gtgaaataaa ttttgacttt gttcaaatat catggttttt
     8821 tatggttcaa actgatgaca ataatcagaa taattagtat attgattatg tggttaatgc
     8881 cacgttaaaa attgaaactg aaaatcgccg atgcagggag cagccactcc ctgcatcgac
     8941 tgtccataga atccttagag gaggttccta tgtgtgcgat ggatcgtaga gaaagagcac
     9001 tcatcagtca attgcatttc cctcatagtc ttggagaggc tatttcgttt ttaaaatatc
     9061 cagtatgtgt ccgcttcgag gatggttctt ttattcagga aaataaccgc tttgaaaaac
     9121 ttatcagatc gtcatttaat tcatgtgatg aatggtttga tagtttaacg ttggaatgta
     9181 aattacagtt atccagagca gagattgaat catgctcttc agtttatggt gtaaattgta
     9241 ataatgacat attacttaat aatatgttat ggagtgtgat catagaaagt gttatcacac
     9301 catgcggcta tttctttatc tggagattta tttgggtagc aaatgataat ctgtcatcgt
     9361 ttgttgtgtc taaatacacc aatgaattaa ttgtactatc tgatgaatat gtaggcattg
     9421 agccatatct gatagggttt agccatcatt attcatcagc aaagatgaac atcactgtgt
     9481 ctaaatcaaa gaagaaaacc atgaaattat ttaaacgaca tggatttagt tctagagatc
     9541 tctggttgga tgaaatgat
//
LOCUS       DAZFQD010000178         9543 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.178, whole genome shotgun sequence.
ACCESSION   DAZFQD010000178 DAZFQD010000000
VERSION     DAZFQD010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9543
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.178"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..818
                     /gene="gadX"
                     /locus_tag="ACHKTW_004607"
     CDS             <1..818
                     /gene="gadX"
                     /locus_tag="ACHKTW_004607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HFO6465505.1"
                     /translation="LHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRVDKC
                     VENFVLVSRDTLSLFLPMLKEEALNLHAHKKISSLLVHHCSRDIPVFQEVAQLSQNKN
                     LRYAEMLRKRALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWTLAR
                     IASELLMSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGYHSV
                     SYFIYVFRNYYGMTPTEYQERSAQGLPNRDSAASIVAQGNFYGTDRSAEGIRL"
     gene            1186..1914
                     /gene="gadW"
                     /locus_tag="ACHKTW_004608"
     CDS             1186..1914
                     /gene="gadW"
                     /locus_tag="ACHKTW_004608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HFO6465506.1"
                     /translation="MTHVCSVILIRRSFDIYHEQHKISLHNESIVLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(2277..5390)
                     /gene="mdtF"
                     /locus_tag="ACHKTW_004609"
     CDS             complement(2277..5390)
                     /gene="mdtF"
                     /locus_tag="ACHKTW_004609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump RND permease MdtF"
                     /protein_id="HFO6465507.1"
                     /translation="MANYFIDRPVFAWVLAIIMMLAGGLAIMNLPVAQYPQIAPPTIT
                     VSATYPGADAQTVEDSVTQVIEQNMNGLDGLMYMSSTSDAAGNASITLTFETGTSPDI
                     AQVQVQNKLQLAMPSLPEAVQQQGISVDKSSSNILMVAAFISDNGSLNQYDIADYVAS
                     NIKDPLSRTAGVGSVQLFGSEYAMRIWLDPQKLNKYNLVPSDVISQIKVQNNQISGGQ
                     LGGMPQAADQQLNASIIVQTRLQTPEEFGKILLKVQQDGSQVLLRDVARVELGAEDYS
                     TVARYNGKPAAGIAIKLATGANALDTSRAVKEELNRLSAYFPASLKTVYPYDTTPFIE
                     ISIQEVFKTLVEAIILVFLVMYLFLQNFRATIIPTIAVPVVILGTFAILSAVGFTINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVIAEDKLPPKEATHKSMGQIQRALVGIAVVLS
                     AVFMPMAFMSGATGEIYRQFSITLISSMLLSVFVAMSLTPALCATILKAAPEGGHKPN
                     ALFARFNTLFEKSTQHYTDSTRSLLRCTGRYMVVYLLICAGMAVLFLRTPTSFLPEED
                     QGVFMTTAQLPSGATMVNTTKVLQQVTDYYLTKEKDNVQSVFTVGGFGFSGQGQNNGL
                     AFISLKPWSERVGEENSVTAIIQRAMIALSSINKAVVFPFNLPAVAELGTASGFDMEL
                     LDNGNLGHEKLTQARNELLSLAAQSPDQVTGVRPNGLEDTPMFKVNVNAAKAEAMGVA
                     LSDINQTISTAFGSSYVNDFLNQGRVKKVYVQAGTPFRMLPDNINQWYVRNASGTMAP
                     LSAYSSTEWTYGSPRLERYNGIPSMEILGEAAAGKSTGDAMKFMADLVAKLPAGVGYS
                     WTGLSYQEALSSNQAPALYAISLVVVFLALAALYESWSIPFSVMLVVPLGVVGALLAT
                     DLRGLSNDVYFQVGLLTTIGLSAKNAILIVEFAVEMMQKEGKTPIEAIIEAARMRLRP
                     ILMTSLAFILGVLPLVISHGAGSGAQNAVGTGVMGGMFAATVLAIYFVPVFFVVVEHL
                     FARFKKA"
     gene            complement(5415..6524)
                     /gene="mdtE"
                     /locus_tag="ACHKTW_004610"
     CDS             complement(5415..6524)
                     /gene="mdtE"
                     /locus_tag="ACHKTW_004610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015870489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtE"
                     /protein_id="HFO6465508.1"
                     /translation="MLTACDDKSAENAAAMTPEVGVVTLSPGSVNVLSELPGRTVPYE
                     VAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELNSAKGSLAKALSTASNA
                     RITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAVEQATINLQYANVTSPI
                     TGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQDFLRMKEEVASGQIKQV
                     QGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIFPNPNGDLLPGMYVTAL
                     VDEGSRQNVLLVPQEGVTHNAQGKATALILDKDDVVQLREIEASKAIGDQWVVTSGLQ
                     AGDRVIVSGLQRIRPGIKARAISSSQENASTESKQ"
     gene            6632..6910
                     /locus_tag="ACHKTW_004611"
     CDS             6632..6910
                     /locus_tag="ACHKTW_004611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6465509.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYQGNKVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(6911..7438)
                     /gene="gadE"
                     /locus_tag="ACHKTW_004612"
     CDS             complement(6911..7438)
                     /gene="gadE"
                     /locus_tag="ACHKTW_004612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HFO6465510.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
     gene            complement(8237..8809)
                     /gene="hdeD"
                     /locus_tag="ACHKTW_004613"
     CDS             complement(8237..8809)
                     /gene="hdeD"
                     /locus_tag="ACHKTW_004613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HFO6465511.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
     gene            9064..9396
                     /gene="hdeA"
                     /locus_tag="ACHKTW_004614"
     CDS             9064..9396
                     /gene="hdeA"
                     /locus_tag="ACHKTW_004614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HFO6465512.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
BASE COUNT         2598 a         2197 c         2197 g         2551 t
ORIGIN      
        1 cattacatgg gaattgtcta attgcgtacg caagacataa atatattctc accatggtta
       61 atggtgaata tcgctatttt aatggcggtg acctggtttt tgcggatgca agccaaattc
      121 gagtagataa gtgtgttgaa aattttgtat tagtatcaag ggatacgctt tcattatttc
      181 tgccgatgct caaggaggag gcattaaatc ttcatgcaca taaaaaaatt tcttcattac
      241 tcgttcatca ctgtagcaga gatattcctg tttttcagga agttgcgcaa ctatcgcaga
      301 ataagaatct tcgctatgca gaaatgctac gtaaaagagc attaatcttt gcgttgttat
      361 ctgtttttct tgaggatgag cactttatac cgctgctttt gaacgttttg caaccgaaca
      421 tgcgaacacg agtttgtacg gttatcaata ataatatcgc ccatgagtgg acactagccc
      481 gaatcgccag cgagctgttg atgagtccaa gcctgttaaa gaaaaaattg cgcgaagaag
      541 agacatcata ttcacagttg cttactgagt gtagaatgca acgtgctttg caacttattg
      601 ttatacatgg cttttcaatt aagcgagtcg cagtgtcctg tggatatcac agcgtgtcgt
      661 atttcattta cgtctttcga aattattatg ggatgacgcc cacagagtat caggagcgat
      721 cggcgcaggg attgccgaac cgtgactcgg cggcaagtat tgttgcgcaa ggaaattttt
      781 acggcactga ccgttctgcg gaaggaataa gattatagaa ttttactcag acataaaaaa
      841 aacccggcat aggggaccgg gaagaggata gtctgccgtc tccagactaa taaaccgtta
      901 taacactccc tgttggcacg ggaaactttg tgctctcagt aagttaaata taacttttac
      961 tggaaataag atcagccatt tttttataaa cataagctat acgcagtgcg aaaatatatt
     1021 cgtgctgcat ttacttatta tcaattaact gttatgcaaa actactttgt ggataaattt
     1081 tggtcctacc aaatctggca gttttttcgc taagaaacag tctggcaaca tttcattagt
     1141 atactgaaat tgaaataatc gcagtatgaa atataaggga taatcatgac tcatgtctgc
     1201 tcggtgatcc tcattcgtcg ttcattcgat atttatcatg aacagcataa aatatcgctg
     1261 cataacgaga gtatcgtgct gctggagaaa aatttggcag acgattttgc gttttgttca
     1321 ccggatacgc gacgactgga tatcgatgag ctgacagttt gccattactt acaaaatatt
     1381 cgtcagctac cacgcaattt agggttacat agcaaagacc gtctattaat taaccagtca
     1441 ccccccatgc cgctggtgac ggcgattttt gatagcttca atgaatccgg ggtaaattca
     1501 ccgatactga gcaatatgct ctacctttcc tgtttatcga tgttttctca taagaaagaa
     1561 ctgatcccct tacttttcaa tagcatcagc actgtttcag gaaaagttga acgccttatt
     1621 agctttgata tcgccaaacg ttggtatctg cgcgatatcg cggaaagaat gtataccagc
     1681 gagagtctca tcaaaaaaaa gttgcaggat gaaaatacct gtttcagtaa aatattactc
     1741 gcttccagga tgtcgatggc cagacgatta ctcgagttac gtcaaattcc tctgcatact
     1801 attgcagaaa aatgtggcta tagcagtacg tcgtacttta taaacacatt tcgacaatat
     1861 tatggtgtaa cgccacatca gtttgcgcaa cattcgccag gtaccttttc ctgacatatt
     1921 ttgcatttga atattggtca ggatctcaca cctgcttcat gtgaaactct tccctgatga
     1981 tttctgccgg gctaccggct agttctcttt cgcagtttgc caggcggtat aggtattttc
     2041 aggaggaaaa gactcggcat gtttgggatt attaagctga caattcatac gattacggga
     2101 atgtgggtca gtattgtgct gttcaaattg atgacgaatg gctggagtgg tttctacttt
     2161 cagtgctgtg tactaagcct ggtattcctg acggttagct ggttgttgag tggtgaatgg
     2221 ttagcaggaa agagtaaggc tgaaccttca cgttcaacct tactctcatt tacacgttac
     2281 gcttttttaa agcgggcaaa gagatgttcc actacaacga aaaagaccgg aacgaagtaa
     2341 attgccagca ctgttgcggc aaacatcccg cccatcacgc cggtacctac cgcgttttgc
     2401 gcgccagaac cggcaccatg actgataacc agcggcagca cgccgagaat aaaggccaga
     2461 gaggtcatca ggattgggcg taaacgcatc cgcgccgctt cgatgattgc ctctatcggc
     2521 gttttccctt ctttttgcat catctcaacg gcaaattcga caatcaggat ggcgttcttg
     2581 gcggaaagcc cgatggtggt cagcaaacca acctggaagt agacgtcatt acttaagccg
     2641 cgcagatcgg tggccagtaa tgcgccaacg acgcctaacg gaacaaccaa catcaccgag
     2701 aacggaattg accagctctc atagagtgcg gcgagggcga ggaacaccac gaccagtgaa
     2761 atggcataca gtgcaggagc ctggtttgag gataacgctt cctgatacga cagtccggtc
     2821 catgagtagc cgacgcctgc cggaagttta gcgaccaggt ctgccataaa tttcatagcg
     2881 tcaccggtac ttttcccggc cgccgcttca cctaaaatct ccattgacgg gatgccgttg
     2941 tagcgttcca atcgcggtga accataggtc cattcggtag acgagtaggc agaaagcggt
     3001 gccatcgtgc cagaggcgtt gcgtacatac cattggttga tgttatccgg caacatacgg
     3061 aacggcgtgc ctgcctggac atacactttt ttcacccgcc cctggttgag gaagtcgttc
     3121 acgtagctgc tgccgaaggc ggtggaaatt gtctggttga tatcagacag cgccacaccc
     3181 attgcctcag ctttcgcggc gttgacgttc actttgaaca tcggcgtatc ttccaggccg
     3241 ttcgggcgta ccccggtgac ctggtcaggc gattgcgctg ccagtgataa cagctcgttt
     3301 cgtgcctggg ttagtttttc gtgccccagg ttaccgttgt ccagcagttc catatcaaaa
     3361 cctgacgcgg tacccagttc agccaccgcg ggtaagttga acgggaagac gacggcttta
     3421 ttgatactgc ttaacgcaat cattgcccgc tgaatgatcg cggtaaccga gttttcctca
     3481 ccgacacgtt cagaccacgg cttgagactg ataaacgcca ggccgttgtt ttgcccctgg
     3541 ccgctgaagc caaagccgcc aacggtaaac accgactgaa cattatcttt ctcttttgta
     3601 agataataat ccgtcacctg ttgcagcact ttcgtggtgt taaccatggt ggcaccggaa
     3661 ggtaactgcg cggtggtcat aaataccccc tgatcctctt ctggtaagaa agaggtcggt
     3721 gtacgcagga acagcaccgc catcccggcg caaatcagca ggtagaccac catgtagcga
     3781 ccggtacaac gcaacagcga gcgggtgcta tcggtatagt gctgagttga tttttcaaac
     3841 agcgtgttga agcgtgcgaa cagggcgtta ggtttgtgac cgccttccgg cgcggctttc
     3901 agaatggtgg cgcacagggc aggggtcagg ctcattgcca caaatactga aagcagcatg
     3961 gaggagatca gcgtgatgga gaactggcgg tagatctccc cggttgcgcc gctcataaag
     4021 gccatcggca taaacactgc ggaaagaaca acagcaatac cgaccagcgc acgttggatc
     4081 tgccccatcg atttatgcgt cgcttccttc ggtggtagct tatcttccgc aatgacacgc
     4141 tcgacgttct ccaccaccac gatggcgtca tccaccagta accctatcgc cagcaccatc
     4201 ccgaacatag ttaacgtgtt gatggtgaaa ccgaccgccg acaagatcgc aaacgtcccg
     4261 agaataacca ccggtacggc aatcgtcggg atgattgtgg cacggaaatt ctgcaaaaac
     4321 agatacatga ccaggaagac gaggatgata gcctcaacca gtgttttaaa aacttcctga
     4381 atagaaattt cgataaacgg cgtggtgtcg taaggataaa ccgtcttcag acttgcgggg
     4441 aaataggctg ataagcggtt cagctcctct ttgactgccc gcgaggtatc cagggcgttt
     4501 gctccggtag ccagtttgat ggcgatcccg gcagcaggtt tgccgttata gcgcgccacg
     4561 gtggaataat cttccgctcc aagttcgacg cgggcgacat cacgcagcag cacttgcgaa
     4621 ccatcttgct gaactttcaa caggattttg ccaaattctt ccggcgtttg cagacgcgtc
     4681 tgcacaatga tcgaggcgtt tagttgctgg tctgccgcct gtggcatgcc acccagttga
     4741 ccaccggaaa tctggttgtt ttgcacctta atctgggaaa taacatcgga aggtaccaga
     4801 ttgtatttat tgagtttttg cgggtccagc cagatacgca tggcgtactc ggaaccaaag
     4861 agctgtacgc taccaacgcc cgcggtacgg cttagcgggt ctttgatatt agacgctaca
     4921 tagtccgcga tatcgtactg gttgaggctg ccgttatcag aaataaacgc cgctaccatc
     4981 agaatattac tgctcgactt atcaacgcta atcccctgct gctgcaccgc ttcaggtaat
     5041 gaaggcatag cgagttgcag tttattttgc acctgaacct gtgcgatatc aggagatgtc
     5101 ccagtctcga aggtcagagt gatagaggca ttacccgccg catcactggt tgaagacatg
     5161 tacatcaggc catcaagccc attcatattt tgctcaatca cctgagtgac cgagtcttct
     5221 accgtttgcg catcggcacc tggataggta gcgctgacgg taatggtcgg tggcgcaatc
     5281 tgcggatact gcgcaaccgg taagttcatg atcgccagac cacctgcaag catcataata
     5341 atggcaagta cccaggcaaa aaccgggcga tcaataaaat agttagccat gaaagtcccc
     5401 ttaagcctgc aacgttattg tttcgattcg gtgctggcgt tttcctggct ggaggaaatt
     5461 gctcgtgctt tgatacccgg acgaatgcgt tgcaaaccgg aaacgatcac ccgatcgcca
     5521 gcctgcaagc cagaggtgac aacccactgg tcgccgatgg ctttgctggc ttcaatttcg
     5581 cgtagctgca caacatcgtc tttatccaga atgagcgccg ttgctttacc ctgggcgttg
     5641 tgggtgacgc cttcctgcgg caccagtaat acattctggc ggctaccttc atccactaat
     5701 gccgtgacgt acatgccagg cagcaagtca ccatttgggt tggggaaaat cgcccgtaac
     5761 gtcacggagc ccgtggtttc atccaccgtc gggtcggaga atttcagcgt gccggtctgg
     5821 ctgtagcgtt taccattttc cagattgagc tgtactggcg tactgccctg aacctgtttg
     5881 atttgcccac tggcgacctc ttctttcatg cgtaagaaat cttgcaccga ctgcgtgaga
     5941 tcgacataaa tcgggtccag acgttgtacg gtaaccagcg aatctgcctg attagcggta
     6001 acgagtgcgc cgacggtcac cgacgatttc ccgctgacgc ccgtaatcgg cgaggtgaca
     6061 ttcgcgtatt gcagattgat cgtcgcctgt tcaacagccg ctttggcgac ggtgacattg
     6121 gcttctgctt cattcaactg ggtgcgcgcg gtatcgtaat cctgacggct aacgtagttg
     6181 gtcttcagca acgatgcctg gcggttaaag gtgatgcggg cattgctggc ggtagagagc
     6241 gctttcgcca gcgagccttt ggcggagttt agctcggcct gtaaaggtgc aggatcaatc
     6301 tgatacagcg aatcgccctg gttcacttta tcgccttcga taaagttgcg tttaatgata
     6361 ataccgccca cctggggacg tatctcggca acttcataag gaacggttct accgggcaat
     6421 tcgctcaaca cattgaccga accgggggag agtgtgacga caccgacctc aggcgtcatg
     6481 gcggcggcgt tttccgccga tttgtcatcg caggcggtga gcatcgcgcc gcagaataac
     6541 aacggtatta acagctttct tcttctgttc attttagtcc ctgaaaattc ttgagaaata
     6601 acggttcttt aaattgcgcg cgaggagaga tatgagggat ttacagacca gcggcatagt
     6661 ggggttgtca gccagtaagg tgggatacag gcacagtgat cgacatggtg aggtcaacga
     6721 caaaaaaaat gttgccgttc tgccaacagt tcctgccagc attcgggcaa cgaagggttc
     6781 tactggtgga tacacatacc aggggaataa agtcgtttta gcctctcctt tagtaatcac
     6841 cgggggtaat gaaatcagca tctgcatccc tcgtcatgcc agccatcaat ttcagttgct
     6901 tatgtcctga ctaaaaataa gatgtgatac ccagggtgac gatgtcgctc atacgtctta
     6961 gtttcagctt gtacataatg ttgaatttct gaatctttaa tgtttgttgc gaaatattga
     7021 gcgtcgacgt gatattgctt tcatttttat ttttcagaat cagttgcata attttgaact
     7081 gattatgtga aaaacataat tgtgaattct tatggggcaa gtgtttacca taagtaatct
     7141 caagaagatt tttgatactt tctttgcggc taacaaaata aataggcgct ttagctttta
     7201 gtttactgat gtgataaggt gccaaaacaa taattctctc ggcatctaat ttctccagaa
     7261 atttaatcgc ttcttcatca aggatatgat tgtgataggt atcaatgata accttgaagg
     7321 gtttattgcc tacttttttg atctctgaca gggaattgat ttttatcatt tcgtgattat
     7381 ctttcaactg ccaaaagccc tgtaaaagaa aagaatcttt cgtcatgaga aaaatcataa
     7441 cttgctcctt agccgttatc gtttcgaaca tgtcatcctt ttcattattt acatccttgt
     7501 ccgaatcgtt gttcaatata gtaaacgcct ataagaactt tgcccttcgt gaactaatac
     7561 gtctattaac actaatcaat acttcagatt tatccatcac gcactccata tattgatcct
     7621 aagtaagatt cctggttgtt atcaatttgt aaatatcaaa caaaaagtta acgtttgcgg
     7681 attactgccc aagaataagt tatcaataac cagtttgtga tctctgaaga atattactaa
     7741 agttaaaatg taatccgatt taaatatcga gtctccttgt ttcgacttaa gctggcaatt
     7801 ggattgccag ctttctttta acgcattgaa attacctgag taaataacgc attcatgtct
     7861 tcaggttttt atatagcgtc tggagacacg gatatttatg caatgtaatc aacgccagtt
     7921 gcaaaaaaat gaacccggat tacttattta accgcccata gcatcaacgc ttacggaact
     7981 ctttgttcac tttcccacag cacttttaaa acattaaacc tgactacgga aaatatcagc
     8041 catgataatg tttgaaatgg ctaatttgcc atggagtgaa aaaaattaga tgaaattcag
     8101 taggttgaaa tgatcactag caggtaatta tttcaatgat agtgcgcaat tgatctacaa
     8161 cactgcgtag cggagagagt attaatcgga tcatagtcac atcaagtgac tatgatccgg
     8221 gtgacaaccg ggataattat tgctgcttaa cgaacaaact ggcgaagctg aacaggctgg
     8281 cggcgctaaa tatcagttca attcccacca gtgtggaaac cagcgttaca gacaccatcg
     8341 gcgttgcacc aaggaatatc caggcaatga cgatatccag cacaccaata acgagctgta
     8401 gccagctgcc tttcattgaa cgctgacgat accaactcat caggcgaata acccctgcaa
     8461 cacagaacaa accggcaata aatgccgcaa tggcaaaaat gcccagctcc ggtgcgcgga
     8521 tgaagaaata gccgatcaat aaataggcga ctgcgacgag gaaaccggat aataccggcc
     8581 agaaattatg actgcggttg ctgaataacc cgacaataag cgcaataccc gagcagatta
     8641 ataatgcacc cactactgtg cttaaaatat cgccagagac gaacgggaaa ctgatacaca
     8701 gcaacccgac gataaacagc agcacggcaa taaactggat tgctctgcga tgctttttca
     8761 gcatctccag atcaaacttc aaaattgttg ccttatctat atataacata gaaccaccct
     8821 ataaaattaa gaagaaaatc ccctgctatc aatctatgcc aaaaacgcgt ctaagaatgc
     8881 agtcgattta ataaaaattt cctaattgca gtatctgatg catctgtaac tcattgtatt
     8941 gaaataaaaa tatctgattt tgatattttc catcaacatg acatatacag aaaaccaggt
     9001 tataacctca gtgtcgaaat tgattcgtga cggctctttc actttatagt tgaggatatt
     9061 acgatgaaaa aagtattagg cgttattctt ggtggtctgc ttcttctgcc agttgtgagc
     9121 aatgcagcgg atgcgcaaaa agcagctgat aacaaaaaac cggtcaactc ctggacctgt
     9181 gaagatttcc tggctgtgga cgaatccttc cagccaactg cagttggttt tgctgaagcg
     9241 ctgaacaaca aagataaacc agaagatgcg gttttagatg ttcagggtat tgcaaccgta
     9301 accccagcta tcgttcaggc ttgtactcag gataaacaag ccaactttaa agataaagtt
     9361 aaaggcgaat gggacaaaat taagaaagat atgtaattcc gggaatgcgt tacatcgtac
     9421 ttccttgcat attgaacagg ccggaatatc ttctttaaaa gcagctattc ctcctgttca
     9481 tatataatct ctatattgaa tgggttacaa aatgaatatt tcatctctcc gtaaagcgtt
     9541 tat
//
LOCUS       DAZFQD010000179         9420 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.179, whole genome shotgun sequence.
ACCESSION   DAZFQD010000179 DAZFQD010000000
VERSION     DAZFQD010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9420
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.179"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..556
                     /locus_tag="ACHKTW_004615"
     CDS             <1..556
                     /locus_tag="ACHKTW_004615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SopA family protein"
                     /protein_id="HFO6465513.1"
                     /translation="DDKFDNFMRQHRFSKVNFDTQQMIHFINRFNMNKGLIDKNNNFF
                     IQLIDQALRSTDDMIKANAWYLYKEWIRSDDVSPIFIETEEKLRTFNTNKLTRDDNIF
                     ILFSSVDDGPVMVVSSQRLHDMLNPTKDTNWNSTYIYKSRHEILPVNLTPETLFGSKS
                     QDKHALFPIFTASWRATRIKNKGI"
     gene            779..1276
                     /gene="ubiC"
                     /locus_tag="ACHKTW_004616"
     CDS             779..1276
                     /gene="ubiC"
                     /locus_tag="ACHKTW_004616"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008813 - chorismate lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HFO6465514.1"
                     /translation="MSHPALTQLRALRYFKEIPALEPQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            1289..2161
                     /gene="ubiA"
                     /locus_tag="ACHKTW_004617"
     CDS             1289..2161
                     /gene="ubiA"
                     /locus_tag="ACHKTW_004617"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HFO6465515.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWILAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSIVVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(2316..4739)
                     /gene="plsB"
                     /locus_tag="ACHKTW_004618"
     CDS             complement(2316..4739)
                     /gene="plsB"
                     /locus_tag="ACHKTW_004618"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004366 - glycerol-3-phosphate
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HFO6465516.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RLYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPVHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEEYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            4910..5278
                     /gene="dgkA"
                     /locus_tag="ACHKTW_004619"
     CDS             4910..5278
                     /gene="dgkA"
                     /locus_tag="ACHKTW_004619"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFO6465517.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            5388..5996
                     /gene="lexA"
                     /locus_tag="ACHKTW_004620"
     CDS             5388..5996
                     /gene="lexA"
                     /locus_tag="ACHKTW_004620"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HFO6465518.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            6069..7394
                     /gene="dinF"
                     /locus_tag="ACHKTW_004621"
     CDS             6069..7394
                     /gene="dinF"
                     /locus_tag="ACHKTW_004621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HFO6465519.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMLKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIVAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            7510..7719
                     /gene="yjbJ"
                     /locus_tag="ACHKTW_004622"
     CDS             7510..7719
                     /gene="yjbJ"
                     /locus_tag="ACHKTW_004622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFO6465520.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(7761..8276)
                     /gene="zur"
                     /locus_tag="ACHKTW_004623"
     CDS             complement(7761..8276)
                     /gene="zur"
                     /locus_tag="ACHKTW_004623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HFO6465521.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
     gene            8594..8848
                     /gene="yjbL"
                     /locus_tag="ACHKTW_004624"
     CDS             8594..8848
                     /gene="yjbL"
                     /locus_tag="ACHKTW_004624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjbL"
                     /protein_id="HFO6465522.1"
                     /translation="MLKIIPGATGYFNKTLNSNQFDNEDAIKDKLDNRGSIKGKLNNI
                     YGKSIDYAALRHRDIIIAKIDLFIQRITHNLWHARKKMCF"
     gene            8872..>9420
                     /locus_tag="ACHKTW_004625"
     CDS             8872..>9420
                     /locus_tag="ACHKTW_004625"
                     /inference="COORDINATES: protein motif:HMM:NF022352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2713 family protein"
                     /protein_id="HFO6465523.1"
                     /translation="MWVNKYIDDCTDEDLNDRDFIASVVDRAIFHFAINSICNPGDNK
                     DATPIERCTFDVETKNGLPSTVQLFYEESKDNEPLANIHFQAIGSGFLTFVNACQEHD
                     DNSLKLFASLLISLSYSSAYTDLAGAEKVNINEYNENYLTAQFEELSQRDMKKYLGEM
                     KHLADRGGMKFDDYLDKMSLLVN"
BASE COUNT         2277 a         2178 c         2479 g         2486 t
ORIGIN      
        1 agatgacaag ttcgataatt ttatgcgtca acatcgattc agtaaggtga atttcgatac
       61 ccaacagatg atccatttta ttaacagatt taatatgaat aaagggctga ttgataaaaa
      121 taacaatttt tttatccaac ttatcgatca ggccttacga tcaacggatg atatgatcaa
      181 agcaaatgcc tggtatcttt ataaagagtg gattcgtagt gatgatgttt cacctatatt
      241 tatagaaact gaagaaaagt taagaacctt taacactaat aaattaacac gagacgataa
      301 tatatttatc ctgttctcct ctgtcgatga tgggcctgtt atggtggtaa gctcccagcg
      361 cttacatgat atgttgaatc ctacaaaaga taccaattgg aattccacgt atatctacaa
      421 atccagacat gagatattgc ctgttaatct tactccggaa acacttttcg gctccaaatc
      481 tcaggataaa catgcacttt tccccatttt tactgcgagt tggcgagcta ctcgtataaa
      541 gaataaaggt atttaagtaa aggaaaacat caccattcct ggcatcatgg gcggtgatgc
      601 ccctatggtt gccctcgcca gcacgggcat cggcaaagcg taaggttcaa cagcgtttta
      661 ccacttcatg cgattgttgc gtttttgttg cgcattagat cacttaattt gctttacatc
      721 tcccgtaaac acttttctgc gatacaatgc ctttacgtta tgtaacggag agttcggcat
      781 gtcacacccc gcgttaacgc aactgcgtgc gctgcgctat tttaaagaga tccctgccct
      841 ggagccgcaa ctgctcgact ggctgttgct ggaagattcc atgacaaaac gttttgaaca
      901 gcagggaaaa acggtgagcg tgacgatgat ccgcgaaggg tttgtcgagc agaatgaaat
      961 tcccgaagaa ctgccgctgc tgccgaaaga gtctcgttac tggttacgtg aaattttgtt
     1021 atgtgccgat ggtgaaccgt ggcttgccgg tcgtaccgtc gttcctgtgt caacgttaag
     1081 cgggccggag ctggcgttac aaaaattggg taaaacgccg ttaggacgct atctgttcac
     1141 atcatcgaca ttaacccggg actttattga gataggccgt gatgccggac tgtgggggcg
     1201 acgttcccgc ctgcgattaa gcggtaaacc gctgttgcta acagaactgt ttttaccggc
     1261 gtcaccgttg tactaagagg aaaaaaatat ggagtggagt ctgacgcaga ataagctgct
     1321 ggcgtttcat cgcttaatgc gtacggataa gccaattggc gcgttactgc tgctctggcc
     1381 aacattatgg gcgttgtggg tggcgacacc gggcgttcct cagctctgga tcctggcggt
     1441 gtttgtcgcg ggtgtctggc tgatgcgcgc tgccggatgt gtggtgaatg attatgctga
     1501 ccgcaagttt gatggtcatg ttaagcgcac ggcgaaccga ccgcttccca gcggcgcggt
     1561 aacggagaaa gaggcgcgcg cgctgtttgt agtgctggta ttgatttcat ttttactggt
     1621 gctgacgctg aatacgatga ctattctgtt gtcgattgcc gcgctggcgc tggcgtgggt
     1681 atacccattt atgaagcggt atacccatct accgcaagtg gtgctgggcg cggcgtttgg
     1741 ctggtcgatt ccaatggctt ttgccgctgt gagtgagtcg gtgccattga gttgctggtt
     1801 aatgttcctc gccaatattc tctgggcggt ggcttacgac acgcagtatg cgatggttga
     1861 ccgcgatgat gatgtgaaga ttggcattaa atccacggca atcctgttcg gccaatacga
     1921 taaattgatt attggtattt tgcagattgg cgtactggca ctgatggcga tcatcggtga
     1981 gttaaatggc ttaggctggg gatattactg gtcaattgtg gtggctggcg cgctgtttgt
     2041 ttatcaacaa aaactgattg ccaaccgcga gcgtgaagcc tgctttaaag catttatgaa
     2101 taataactat gttggtctgg tactattttt agggctggca atgagttact ggcatttctg
     2161 atgatgtaaa aaagccggat gatcatccgg ctttcttctg ggttgcctga tgcgcggcgc
     2221 ttctcaggcc tacacaacac atcgcaattt attgaatttg cagattatgg aaggccggat
     2281 aaggcgtttt cgccgcatcc ggcaattctc tctgattacc cttcgccctg cgtcgcactc
     2341 tcaatcgtca aacgcacgtc tgatgtaatc aactccgcca gcaactgata aaccttcatc
     2401 gtttctgccg gttcggcatc gccgctatcg ctgatatact cttcatcacg cagtgtcagc
     2461 accagagaac tgaacaccgc cttgtcgaag aactccggcg cgttgatgcc gtgcagcacg
     2521 gagagacgtt gcgcgacggt gcggctctct ttctccagcg taccgcggtt gatcgacggg
     2581 ttggcactca acaaccagaa ggtgatggca taacgttgca gcgtttcgcg cgcgcctgcg
     2641 gcgagcagct gtagcgtgcg agaatgcacc gggttgatat gcaactcatc atcttgcagg
     2701 gtaatcagcc cctgacgttg catctcattt gccagcgcat caataacgtc cggcaactcg
     2761 tcgcgatccc agcgcaggaa cagctccgct ttcagcattg ggtaaagcac attgacgtgc
     2821 tccatcaata cgtcgcggga gatgtggcga tgctgggtga caattgccgc catcagcgaa
     2881 ggcagcacca acatatgcgc aatgttgttg cgatagtagg tcatcagcac cgcttgctcg
     2941 cgtggcagaa tgatgatgtc gccgatagtg tctttctcga cttcaaactt gttcatttgc
     3001 agcgcgtgat cgataagctc gctggcgctg gctgaaggaa cggtagagtc cgtggagtaa
     3061 ggtacgttgc gcatcagatc caggtagcag ttgagttgct cggttaactg ctcgcgggtg
     3121 agtgagcgct gacgtgatgc cagtagcgca gtacagcaca ggttcatggc gtttgccgcg
     3181 cctgcgttgt taatgcgtac catcagatcg gcagcaatat tattgaccgt cggcgttaac
     3241 caggccggac gcaccgcttc gatgggatcg atagattcac gccagtctgg tacgtgctgg
     3301 ttaaggtagg tcatcaacgg cattggttca ccgaagttga cgtaaccctg accgagatta
     3361 cgcagcttgc ttaaaccgcg cagcatctgc ggcaggctct ctttctcttt cgtcgcgccg
     3421 cgcagttctt tggcgtaagt acccacttcc atgacgtgct cataaccgat atagatcgga
     3481 atcagcgtaa tcggacgcgt gccgccacgc agcatcgcct gaatggtcat cgacagcgta
     3541 ccggttttcg gatccagcaa acgccccgtg cgggaacgac cgccttccac gaagtactcg
     3601 acggaataac cacggctgaa cagttcgccg agatactcac ggaaaacggt ggaataaagt
     3661 ttattgcctt taaaagtacg gcgaataaag aacgccccca gacggcggaa aatcggcccg
     3721 gccggccaga agttcaggtt gatcccggcg gcgatatgcg gcggcaccag cccctggtgg
     3781 tacagcacgt aagaaagtag caggtagtcc atgtgactgc ggtggcaagg cacatacacc
     3841 agctcatgac cgtcgtgggc cagctggcga acgcgctcgg cgttatggac gttgatgccc
     3901 tgataaagtc ggttccaggt gaagcccaga atgcggtcag tcaggcggat catctcgtaa
     3961 gagaaattcg ccgcaatctc ttccatcagc gcaatcgcgt tctgctgcgc tttttcatgg
     4021 gagatttttt tgctgcgcgc ttcatcttct accgctttgg caatggcgcg ggaggcgagc
     4081 agcttattaa acagatcctg acgagcaggc agacgtgggc ccacagcagc cagacgttga
     4141 cgggcaaagt gcatacgcgc cacgcgcgcc agtttctgtg cgatagtttt atccgtgccg
     4201 tgttcatccg ccatacggcg cagcgaaact gacggcgaga aacgcacaaa actgtcgcga
     4261 ccgagccaca gtacggcgaa aaatttctgt acgccgttaa gcatacgcag cggcgggttt
     4321 acttcgcctt tttcacgccc cggcgcgcga ccaaacatca ccgatactgg caccatctgc
     4381 acatccagat ttgggttgct acggtgcaaa tcgagatagt cgtggaacag cttaatagac
     4441 tcttctttcg gcgtgtaata ggtgaataca cgcggcccgc cgtgaatgaa cacatagcgc
     4501 ggcagtagcg tgccgtcgat ttccagcggc tctaacgggt caggcaagtc atgagccaga
     4561 cactgggcgc gtaacgtcag caaatctgct ttcgagttgt acggtaaaac gtacataatt
     4621 ggacgagaag tatccagccc cagttccggg gcaggatctg ccggaataga cttgcttttt
     4681 accaggatgc ttaatggtaa attcagtaat ttgtagtaaa ttcgtggcca gccggacata
     4741 aacgatgtaa agcctctggt taataatgca aatgcgcggc aaggatagca gaaagtcatg
     4801 ggaaattctg tggtatccgc tcatgtttcg cgcggcgtta cgcaaacccg aatcatcgga
     4861 tttaacggta cactgatatt gacgctcata atgtaaaaag gttctttcaa tggccaataa
     4921 taccactgga ttcacccgaa ttatcaaagc tgctggctat tcctggaaag gtttacgcgc
     4981 tgcatggatc aacgaagcgg cattccgtca ggaaggcgta gcggtattgt tggcggtggt
     5041 catcgcctgc tggctggatg tggacgcaat tacccgcgtg ctgcttatca gctccgtgat
     5101 gctggtgatg attgtggaaa ttctcaatag cgccatcgaa gcagtggttg accgaattgg
     5161 ctctgaatac catgagcttt ccggacgcgc taaagatatg ggatccgccg cggtgctgat
     5221 tgccattatc gtcgccgtga ttacctggtg cattctgtta tggtcgcatt ttggataacc
     5281 cttccagatt tcgataaatc tctggtttat tgtgcagttt atggttccaa aatcgccttt
     5341 tgctgtatat actcacagca taactgtata tacacccagg gggcggaatg aaagcgttaa
     5401 cggccaggca acaagaggtg tttgatctca tccgtgatca catcagccag acaggtatgc
     5461 cgccgacgcg tgcggaaatc gcgcagcgtt tggggttccg ttccccaaac gcggctgaag
     5521 aacatctgaa ggcgctggca cgcaaaggcg ttattgaaat tgtttccggc gcatcacgcg
     5581 ggattcgtct gttgcaggaa gaggaagaag ggttgccgct ggtaggtcgt gtggctgccg
     5641 gtgaaccgct tctggcgcaa cagcatattg aaggtcatta tcaggtcgat ccttccttgt
     5701 tcaagccgaa tgctgatttc ctgctgcgcg tcagcgggat gtcgatgaaa gatatcggca
     5761 ttatggatgg tgacttgctg gcagtgcata aaactcagga tgtacgtaac ggtcaggtcg
     5821 ttgtcgcacg tattgatgac gaagttaccg ttaagcgcct gaaaaaacag ggcaataaag
     5881 tcgaactgtt gccagaaaat agcgagttta aaccaattgt cgttgacctt cgtcagcaga
     5941 gcttcaccat tgaagggctg gcggttgggg ttattcgcaa cggcgactgg ctgtaacata
     6001 tctctgagac cgcgatgccg ccaggcgtcg cggtttgttt ttcatctctc ttcatcaggc
     6061 ttgtctgcat ggcattcctc acttcatctg ataaagcact ctggcatctc gccttaccca
     6121 tgattttctc caatatcacc gttccgttgc tgggactggt cgatacggcg gtaattggtc
     6181 atcttgatag cccggtttat ttgggcggcg tggcggttgg tgcaacggcg accagctttc
     6241 tctttatgct gttgctgttt ttacgcatga gcaccaccgg gctgactgcg caggcttatg
     6301 gtgccaaaaa tcctcaggca ttagcccgtg cgctggtgca accgttgctg ttggcgttgg
     6361 gggctggggc gttaattgcg ctgctgcgta caccgattat cgatctggcg ctgcatattg
     6421 ttggcggtag tgaagcggtc ctggaacagg cgcggcgctt tcttgaaatc cgctggttaa
     6481 gcgctccggc gtcgctggcg aatctggtat tactcggttg gttactcggc gtgcaatatg
     6541 cccgtgcgcc agtaattttg ttagtggtcg gcaatatcct caacattgtg ctggatgtct
     6601 ggctggtgat ggggctgcat atgaacgtgc agggcgcggc gctggcgacg gttattgcgg
     6661 aatatgcaac attgctgatt ggtctgctaa tggtgcgtaa aatcctcaaa ctacgcggaa
     6721 tttccggcga aatgctgaaa actgcctggc gaggaaactt ccgtcgcttg ctggcgctta
     6781 accgcgatat catgctgcgt tcgctgttgt tgcaactctg tttcggcgcg atcaccgtac
     6841 ttggtgcgcg actggggagt gacattgtcg ctgttaacgc ggttctgatg acgctactca
     6901 cctttaccgc ctatgcgctt gatggttttg cctacgcggt tgaagcgcac tccggtcagg
     6961 catacggtgc gcgcgacggt agccagttgc tggatgtatg gcgggcagcg tgccgccagt
     7021 cggggattgt agcgttactg ttttcggtgg tttatttgct ggcaggggaa cacatcattg
     7081 cgttgctgac gtcgttaacc cagattcagc agctggctga ccgctatctt atctggcagg
     7141 tgattttgcc gttggttggc gtctggtgtt atctgctgga cggcatgttt ataggcgcaa
     7201 cgcgtgccgc cgaaatgcgt aacagtatgg cggtggccgc cgcaggtttt gcgctgacgc
     7261 tccttacgct gccgtggctg ggtaatcatg gtttgtggct ggcattaacc gtctttctgg
     7321 cgttgcgcgg gctttctctg gcggctatct ggcggcgtca ctggcgcaac ggtacctggt
     7381 ttgccgcaac gtgacggtta aaaattctga ataaattatc ctaagccaaa ttgctgacta
     7441 cacttaatct cacgttcaga agaaaagtga acgtactctc attcacaacc taacgatgag
     7501 gtcttgatta tgaataaaga tgaagccggc ggtaactgga aacagtttaa aggtaaagtg
     7561 aaagagcaat ggggcaaact gaccgatgat gatatgacga tcattgaagg taaacgtgat
     7621 caactggtcg gtaaaatcca ggaacgttat ggttatcaga aagatcaggc agaaaaagag
     7681 gtcgtggatt gggaaacccg caatgaatat cgctggtaat taatccctcc tgcccgacgt
     7741 gtacaaggat gtacgccctc ttaacgcggt ttctttttca cctgcacaga gtgatcatgc
     7801 tggcactgtt caggatgacg acacgcttcc acttccacgc atgccgcaca gagcccatgt
     7861 gcttcaatca cattatgccg cagggcaaac cccatttttg ccgccagcgt atgcataatg
     7921 tcttccacgc cttctgcaca ctcttctttc accgcgccgc agcgatcgca tataaacatg
     7981 gctgacgtat gcgtgggctg gtcgaacaga tgacagagca cataactgtt ggtggattcc
     8041 accttatgca caaaaccttg ctcaagcaga aaatccagcg cgcgataaac cgttggcggc
     8101 ttggcttgcg gttcagcttc gcgcagtaaa tcaagcagat cataagcgct gatagcgcca
     8161 tcctgcagac tcatcaggcg caacacttcc aggcgctgtg gggtcaggcg cacattacgc
     8221 tgcgcgcaga ttttttcagc ctgcgctaat aactcctgcg ttgtggtctt ttccatcggg
     8281 cacctcaaag tgggggttaa gaatgtcctt actttaccat gttccaggaa aaacaccgag
     8341 aacccttatt gttgccgtaa tgttgatttt ctgttttgta ggtaaggtgt tgtgttgcct
     8401 tgtcgtacca ttatcaacac gataataatt aataattcat ttttaaataa attgcttcaa
     8461 gttaactcgc tatttattta actgggttta aacgcatacc tgtagttcag aaattatgag
     8521 cgatttttga tagtgaccag atggcaaaag aattataagg aggacacggt acgacaaaaa
     8581 aggtagatga atcatgttga agataatacc cggtgcaact ggatacttta ataaaacact
     8641 caattcaaat caattcgata atgaagatgc aatcaaagat aaattagata atagaggttc
     8701 aatcaaaggt aaattaaata atatatatgg taaatctatt gattatgcag cgctgcgtca
     8761 tcgggatata attattgcaa aaatagattt gtttattcaa cgaatcacgc ataatttatg
     8821 gcatgcacgt aaaaaaatgt gtttttaatt gagcaaataa atgatctcaa aatgtgggtc
     8881 aataagtata ttgacgattg cactgatgag gatttaaacg atcgtgactt tattgcatca
     8941 gttgttgacc gggctatttt tcatttcgcg attaatagta tatgtaatcc tggggataat
     9001 aaagatgcga ctcccattga acgatgtact tttgatgtag aaactaagaa tggccttccc
     9061 tccacggttc agctatttta tgaggaatct aaggataatg aacctttagc gaatatacat
     9121 tttcaagcaa taggttctgg ttttttaacg tttgttaatg cctgccagga acatgatgac
     9181 aacagcttaa aattatttgc ttcgctgtta atttcacttt catattctag tgcctacaca
     9241 gatttagccg gagcagaaaa agtgaatatt aatgaatata atgagaacta tctgacagct
     9301 cagtttgaag aattatctca acgtgatatg aagaagtacc tgggagagat gaagcatctg
     9361 gcggaccggg gggggatgaa atttgatgac tatctggata aaatgtcact tctggtgaat
//
LOCUS       DAZFQD010000180         9343 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.180, whole genome shotgun sequence.
ACCESSION   DAZFQD010000180 DAZFQD010000000
VERSION     DAZFQD010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9343
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.180"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            329..907
                     /locus_tag="ACHKTW_004626"
     CDS             329..907
                     /locus_tag="ACHKTW_004626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilL N-terminal domain-containing protein"
                     /protein_id="HFO6465524.1"
                     /translation="MKTSLLLSVLSACVLLTGCQGQQKLLQRDAAVPVPSVTVSNNVR
                     PASPDMYAGTTVPEVVRYDRYVLVNTHPDAVQRDPLSQVIDIRIPASLKPTVADAMRY
                     ALKQSGYTLCATGPANGVLYRQALPAVQYQTGPLRLRTALQMMAGPAWQLEVDDVQRV
                     VCHSLREGYQLPPGQLAPVPAGVRDAGAGLKK"
     gene            904..1671
                     /locus_tag="ACHKTW_004627"
     CDS             904..1671
                     /locus_tag="ACHKTW_004627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6465525.1"
                     /translation="MSEKKQKTLPSVHSSPLDGWTCVSPMLSVGFVLTVLVATILAVC
                     GLYRYSGEQDERIAALEGQMKTYSDAFALSGSELEPLRSRLAETEKQLKSVTERLTLL
                     EQRPVLTEEEHARTGQMAASLASLQATLAQQGETLRAYDRRLEKLEKAPFTPVSPSSA
                     KGTGTVKSTPAVTPRQKNGSSGVSVISRSAPFVLTGTEQRGGRTYAAVAPRGFTRLSD
                     VTLLGEGESTAGWMLERAGRGEASFRVNGRQVRVMVE"
     gene            1676..2398
                     /locus_tag="ACHKTW_004628"
     CDS             1676..2398
                     /locus_tag="ACHKTW_004628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006785960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03759 family integrating conjugative element
                     protein"
                     /protein_id="HFO6465526.1"
                     /translation="MKMKNLSPVLMLLISLQTVAAVRESAPEGTRTQASSQVSTTTQA
                     LQQQAAQWGVTEEDYRRYRALMEGPRGIQSPGLDPLTVLGIEARTDAERRQLAEKWVK
                     AEYERTEKELAFQREVNAAWKRLYPGVMPVSLGKGGGLLNDNGGRLALFVKAKDCGTC
                     DARLASVLSSGRQVDIYLVDSQGSDNLLRQWALEHDIPVDRVRSRHITLNHDAGRWLR
                     FGEGRMPVVLQQGADGWRVAAF"
     gene            2377..3039
                     /locus_tag="ACHKTW_004629"
     CDS             2377..3039
                     /locus_tag="ACHKTW_004629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase"
                     /protein_id="HFO6465527.1"
                     /translation="MARGSLLTMALLSMAVSTLCRAGAHDIPEGYVRVAAVHGVPAEV
                     LYAVSLTETVMAPSAIAGVMKKRDADFRLPSVARPWPWTINVAGKGYRYASRLEAWQA
                     LQVFLTRYPARRIDVGIAQVNLGWNGHRFSSTWAAFDPYISLNTAATILSECRRRHPV
                     SWLQAAGCYHHPAGGAPAVRYTAVVRQHIERLHRDAVKTAEQHDGLTAMHPRYIWTEP
                     RE"
     gene            3060..3566
                     /locus_tag="ACHKTW_004630"
     CDS             3060..3566
                     /locus_tag="ACHKTW_004630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrating conjugative element protein"
                     /protein_id="HFO6465528.1"
                     /translation="MTAVWPLLLCAAQAGAELRVIADIGGSDASVFYEAINRQEGSST
                     PPPVTAPLPAQGETLALPVTTPEMTPGEVTPRPLQLPGIGALFLVGDDALSRQWLQAN
                     AAALRERQAVGMVVSVADENSLRTLRELAPGVMMVPSSGSALARRLQLNHWPVLITDS
                     GISGVVTP"
     gene            3566..4165
                     /locus_tag="ACHKTW_004631"
     CDS             3566..4165
                     /locus_tag="ACHKTW_004631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000946004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HFO6465529.1"
                     /translation="MLRQMTDIVPDKIPYGVLLCVLTVLFLLFPVLCGRRRRRERASE
                     WRHRHYRRKAERVLAQLSSLPEGGAGVKYLRKINPYVFEELLLLAFERRGMRVIRNSR
                     YSGDGGLDGQVLIDGERWLIQAKRYGRTIRPSHIDDFGELLRREGCRGFFVHTGRTGP
                     ASRARLSLWPDIELISGQRLLKLLNGRLLTGELTGEKRR"
     gene            4179..4967
                     /locus_tag="ACHKTW_004632"
     CDS             4179..4967
                     /locus_tag="ACHKTW_004632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001103052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6465530.1"
                     /translation="MNVSFTEQVQRNMPQWANEPLRAWNAAEFAAMSDFITEHYWTGQ
                     GSINVFRIVGTDHPQYAGMSWLELLERGKRMDINIPLVEKNPGYYTQAEQLHAGMSFV
                     STDGIHWYVSADGNHRSCLARFLFHLQGEERTQLHNVAQSIYHADRAFWSACREIHNL
                     ADVLSRHGVYLRLQTRRQCVSREDLACWKVDRFSTEAQLTVDDGRAGGHASAVYKVLL
                     LSAEDAWREVRKLQNRAEALSASPETDIPRSWWLRLLQRGRGHE"
     gene            4960..7077
                     /gene="traD"
                     /locus_tag="ACHKTW_004633"
     CDS             4960..7077
                     /gene="traD"
                     /locus_tag="ACHKTW_004633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system coupling
                     protein TraD"
                     /protein_id="HFO6465531.1"
                     /translation="MSNRYVIEALLRPAVEFNTAVVAATAAGICVTAPWAVALAPSVS
                     YVTAAGFGVLAAVRFRQGMKIIRYRRNLRRLPRYVMSTRQIPVSRQRLFLGRGFRWTQ
                     KHTQRLQDTLRPEVAHYLQPGGLYRAARWLEMKTEHRLPGLGQWLRRDSPLNPVRPLP
                     PVGGNPALHGIEPDEQDVTLALGERVGHTIVYGTTRVGKTRLAELLVTQDIRRGEVTI
                     VFDPKGDADLLLRVWAEAHRAGRGDELYIFHLGWPEISARYNAVGRFGRVSEVASRVA
                     GQLSGEGNSAAFREFAWRFVNIIARALVALGERPDYMLIMRYVNNIADLYIRYAGKVI
                     RERLPGLEQIIANNQSVLSEDDVPRTMQNQPDAVRIWSIEMALSSEEGKKIYDPILDG
                     LRSAVRYDRTYFDKIVASLLPLLEKLTTGRIAELLAPDYLDMSDARPIFDWEQVIRKK
                     AVVYIGLDALSDSEVASAVGNSMFADLVSVAGHIYKHGMHAGLPAGGAGKNVVNLHCD
                     EFNELMGDEFIPLINKGGGAGMQVTAYTQTSSDIEARIGNAAKTAQVQGNFNNMIMLR
                     VRENRTAELLTTQLPQVEIYTKTLVSGHQDTADVSAGQDFTSSTQDRVGTVKVPLLEP
                     ADIVTLPKGQAFALLEGGQLWKIRMPLPAGDTDDVLMPESISRVAEAMRQNYHSGEGW
                     WNAPPSVQAIATEEGGDHGGSTS"
     gene            7058..7819
                     /locus_tag="ACHKTW_004634"
     CDS             7058..7819
                     /locus_tag="ACHKTW_004634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03747 family integrating conjugative element
                     membrane protein"
                     /protein_id="HFO6465532.1"
                     /translation="MAEARRDAERSPQSRPPRQHGLLYNLVWGWPWALVGLLLSSLLL
                     SLLIEYAGMTFFWPDEGASHSRAVMNTELGWLSTEFTRSLLLSTPSVTVPGWISVAYQ
                     WLFVESGMQSWLQSQQAVTAAGRSVSQFSGELSQWLAYHLHDYLLATVYVTVVTMVRV
                     IILALSVPLFVMVILVAVVEGLGRRDLRRYGAAYESSFVYHHAKKSIKPAIYIPCILY
                     LSWPTAVYPNLLIWPAAMLLGMAVTVTMASFKKYL"
     gene            8509..8793
                     /locus_tag="ACHKTW_004635"
     CDS             8509..8793
                     /locus_tag="ACHKTW_004635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016190711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RAQPRD family integrative conjugative element
                     protein"
                     /protein_id="HFO6465533.1"
                     /translation="MMLLVTLVLPVQAAEKDELATVMRQLDQVRAALDRARTASLQAT
                     PHDGRYFFDYPRAQADIQTMQEGIDRYLTPSRAQPRNGSAVTGEYRRERP"
     gene            8793..9035
                     /locus_tag="ACHKTW_004636"
     CDS             8793..9035
                     /locus_tag="ACHKTW_004636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03758 family integrating conjugative element
                     protein"
                     /protein_id="HFO6465534.1"
                     /translation="MALTSAQAAAFKVASGDVDLNVLHLSLLGLLIAVLFLWAAWGLS
                     DVWSGWRNGDVRESAFIWFTARTVLLLVASIWMFAG"
     gene            9105..>9343
                     /locus_tag="ACHKTW_004637"
     CDS             9105..>9343
                     /locus_tag="ACHKTW_004637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03745 family integrating conjugative element
                     membrane protein"
                     /protein_id="HFO6465535.1"
                     /translation="MARLAGMAMVTFLLGSLTPALADLPQIEPPSSGGGGGLFGQIKG
                     YFQDGIVLIGLVIAAAAFVKVAEAAVTTFSEVRDG"
BASE COUNT         1891 a         2357 c         3008 g         2087 t
ORIGIN      
        1 gcagaagagc gcaaaaaagc gcttgaagcc acttatgcat ctgtccgccg catgagtgat
       61 atggggatgt cgacagaagt cattaagcag gcacttcagc tttctgatga gcagattcgg
      121 gaagctcttc ataattaact tttcatcata atgatgtggc aaagcgcgac tgatgaatca
      181 gaatgcgtta tgccctgtaa acaaagcgcc tgctattgtg caggcgtttt ttttgtcaac
      241 cggaaatcag ttgttcattg agccggtctg gacttcttct gcaaattgac ctctgacttt
      301 atcacttcac cgcagaggta acttcctgat gaaaacatcc cttcttcttt ccgtgctgtc
      361 cgcctgcgta cttctgaccg gctgtcaggg gcagcaaaaa ctgctgcagc gggatgcggc
      421 tgttccggta ccgtcggtga cggtcagcaa taatgtccgt ccggccagcc ctgatatgta
      481 cgcggggacg accgtgcccg aggtggtgcg ttatgaccgc tatgtgctgg ttaacactca
      541 tccggatgcg gtacagcggg atcccctttc ccaggtgatt gacatccgta ttccggcatc
      601 cctgaagccg acggtggcag atgcgatgcg ttatgccctg aaacagtcgg gctacacgct
      661 ctgtgccacc ggaccggcca acggggtgct ttatcgtcag gcgttgccgg cagtgcagta
      721 tcagacgggg ccgctcaggt tgcgtaccgc cctgcagatg atggccggtc cggcgtggca
      781 acttgaagtt gatgatgtgc agcgggttgt ctgtcacagc ctgcgggaag gttatcagtt
      841 gccgccgggc cagctggcgc cggtaccggc cggtgtcagg gacgcaggag cggggcttaa
      901 aaaatgagtg aaaaaaaaca gaagacatta ccgtcagtgc attcatctcc tcttgatggg
      961 tggacctgtg tgtcacccat gctttccgtg ggatttgtac tgaccgttct ggtggcgacc
     1021 attctggcgg tttgcggcct ctatcgttac agcggggagc aggatgaacg cattgccgca
     1081 ctggaggggc aaatgaaaac gtattcagat gctttcgcgt tgtcgggaag tgagttggaa
     1141 ccgctcaggt cccggctggc tgaaaccgaa aaacagctga aaagcgtgac ggagcgactg
     1201 acattgctgg agcaacgacc tgtactgacg gaggaagagc atgcacgcac tggtcagatg
     1261 gccgccagcc tggcatcact gcaggccacg ctggcgcagc agggagaaac gcttcgtgcg
     1321 tatgaccggc gactggagaa gctggagaag gcgccattca ccccggtatc accgtcatct
     1381 gcgaaaggaa cgggcacggt gaagagcaca ccggcggtca cgccacgcca gaaaaatggc
     1441 tcttccggcg tttcagttat cagccgcagc gcaccgtttg tgctgacagg cacggagcag
     1501 cgtggcggac gcacatatgc tgcggtggct ccccgtggat ttacccgtct ttcagatgtg
     1561 acgctgctgg gtgaagggga gagcacggcc ggctggatgc ttgagcgtgc cggacgggga
     1621 gaagcctcgt tccgggtgaa cggacgtcag gtccgggtca tggtggagtg atgaaatgaa
     1681 aatgaagaat ctgtcaccgg tgctcatgct gctcatttcc ctgcagacgg tggctgccgt
     1741 acgggagagt gcgcctgagg gcacacgcac gcaggcctct tcgcaggtca gtaccaccac
     1801 acaggcgctg cagcaacagg ccgctcagtg gggagtcact gaggaggatt accgtcgata
     1861 ccgggctctg atggagggac cgcgggggat tcagtccccc ggtctggatc cgctgacggt
     1921 gctggggatt gaagcacgga cggatgcgga acgccggcag ctggctgaaa aatgggtgaa
     1981 ggccgaatat gagcggactg agaaggaact ggcatttcag cgggaagtca atgcggcctg
     2041 gaaacgcctt tatccgggcg ttatgccggt cagcctgggg aaagggggcg ggctgctaaa
     2101 cgacaacggc gggcggctgg cgttgtttgt gaaagcgaaa gactgtggaa cctgtgatgc
     2161 acgactggca tcagtgttgt cttcggggcg gcaggtggat atttatctgg ttgacagtca
     2221 gggcagtgac aatctgctgc ggcagtgggc actggagcac gatatccctg tggaccgggt
     2281 gagaagccgc catattaccc tgaatcatga tgccggtcgc tggctgcgtt ttggtgaagg
     2341 cagaatgccg gttgtactgc agcagggagc ggacggatgg cgcgtggcag ccttctgaca
     2401 atggcgctgt tgagcatggc tgtcagcact ctctgccggg cgggggcgca tgatatccct
     2461 gaggggtatg tgcgcgtggc tgccgtgcat ggtgtgcctg cagaagtgct ctatgccgtc
     2521 tctctgacag agacggtgat ggcgccctct gcgattgccg gtgtgatgaa aaaacgggat
     2581 gcggatttcc gccttccgtc cgttgcacgt ccctggccgt ggaccatcaa tgtggcgggt
     2641 aagggctacc gctatgccag tcgcctggaa gcctggcagg cacttcaggt ttttctgaca
     2701 cggtatccgg cccggcggat tgacgtggga attgctcagg tcaatctggg ctggaacggg
     2761 caccgtttct cctccacatg ggcggcattt gatccgtaca tcagtctgaa caccgcagcg
     2821 accatcctga gtgaatgccg tcgtcgtcat cctgtcagct ggctgcaggc ggccggttgt
     2881 tatcatcatc cggcaggtgg tgctccggca gtacgctaca ccgcagtggt cagacaacat
     2941 attgagcgtc ttcatcgtga tgcggtgaag accgcagagc agcatgacgg cctgaccgcc
     3001 atgcatcccc gttatatctg gacagaaccc cgggagtgat accagtgaaa cgatatgtaa
     3061 tgacggcagt gtggcccctg ctgctgtgcg cggctcaggc tggcgcggaa ctcagggtta
     3121 ttgcggacat tggcggcagt gatgcctccg tgttttatga ggcaatcaac cgccaggagg
     3181 ggagcagtac gcctcccccg gttacggcgc ctctgccggc gcagggagag acgctggcgc
     3241 tgcccgtgac cacgccggaa atgacgccgg gtgaggtgac gccgcgacca ctgcagttgc
     3301 cggggatcgg ggcgctgttt ctggtgggag acgatgcgct ttcccgtcag tggctgcagg
     3361 cgaatgcggc cgctctccgt gagcggcagg ctgtcggtat ggttgtcagc gtggcggatg
     3421 aaaacagcct gagaacgctg cgtgagctgg cgcccggcgt gatgatggta ccatcatcgg
     3481 ggtcagcact ggcacgccgt ctgcagctga atcactggcc ggtactgata acggacagcg
     3541 gaatatccgg ggtggtgaca ccgtgatgct gcggcaaatg acggatattg tgccggataa
     3601 gataccgtac ggcgttctgc tgtgtgtcct gaccgtgttg ttcctgcttt tccccgtcct
     3661 gtgtggtcgc cgccggcgtc gtgaacgcgc cagtgagtgg cgtcaccgcc attaccggcg
     3721 caaggctgag cgtgtgctgg cgcaactgtc gtcgttgccg gagggcggtg caggcgtgaa
     3781 atacctgcga aaaattaacc cgtatgtgtt tgaagaactg ctgcttctgg cgtttgaacg
     3841 caggggaatg cgggttatcc gcaattcccg ctacagcggc gacggagggc tggacggaca
     3901 ggtgctgatt gacggcgagc ggtggctgat tcaggccaaa cgctatggcc gcaccatccg
     3961 accgtcacat atcgatgatt ttggtgaatt actgcgtcgt gaaggttgtc gtggattttt
     4021 tgttcacacc gggcgtacgg gaccggccag ccgggcacgg ctttcgctgt ggccggatat
     4081 tgaactgatc agcggacagc gtctgctgaa actgttgaac gggcgattac tgaccggtga
     4141 gctcaccgga gaaaagcgaa gatgacagga gagcggggat gaacgtttca tttactgaac
     4201 aggtgcagag gaatatgccg caatgggcga atgaaccttt acgggcatgg aatgcggcag
     4261 agtttgcggc aatgtcggat tttattacgg aacactactg gacggggcag ggcagcatca
     4321 atgtgttccg catcgtggga actgaccatc cacagtatgc cggaatgagc tggctggagt
     4381 tgctggagcg cggtaagcgt atggatatca atatcccgtt ggtggagaag aacccggggt
     4441 attacacgca ggcagaacaa ctgcatgccg ggatgagttt tgtcagcacg gatggcattc
     4501 actggtatgt cagtgctgat ggtaatcacc gcagttgtct ggcccgcttt ctgtttcacc
     4561 tgcagggaga ggagcgcaca cagcttcaca acgtggccca gagcatttat cacgcagacc
     4621 gggcgttctg gtctgcctgt cgggaaattc acaacctggc tgatgttctc tcccggcatg
     4681 gtgtgtatct tcgtctgcag acccggcggc agtgtgtttc ccgtgaagac ctggcctgct
     4741 ggaaagtgga ccgtttcagc acagaagctc agttgacagt ggatgacgga cgggctggcg
     4801 gacatgcgtc tgcggtgtat aaggtattgt tactgagtgc agaagacgca tggcgggagg
     4861 tgaggaaatt acagaacaga gctgaggcgt tgtcagcgtc accggagaca gatatccccc
     4921 gctcatggtg gctgcgcctt ttacagcgag gacgcggaca tgagtaaccg ttatgttatt
     4981 gaggcgttac tgaggccagc tgtggagttc aatacggccg tggttgcggc aacggcggcc
     5041 ggtatctgtg tgacagcccc ctgggcggtg gcgcttgcgc cttcggtcag ttatgtcaca
     5101 gctgccggat ttggcgttct ggcggctgtc cgtttccgcc agggcatgaa aatcatccgc
     5161 taccgacgca atctgcgtcg gttaccccgc tatgtgatga gcacgcggca aattcctgtc
     5221 agccgtcagc gactgtttct ggggcgggga tttcgctgga cacagaagca tacccagcgt
     5281 ctgcaggata cgctgcggcc ggaggtggcg cattatctgc agcccggagg actttaccgg
     5341 gctgcgcgct ggcttgaaat gaaaacggaa caccggctgc ccgggctggg gcagtggctt
     5401 cgtcgtgatt cgccgctgaa cccggttcgt cccctgccac cggtgggcgg gaatccggcc
     5461 ctgcacggta ttgaacctga tgagcaggat gtgacactgg cactgggtga gcgtgtgggg
     5521 cacaccattg tctacggcac cacgcgtgtg ggtaaaaccc gtctggcaga gttgctggtc
     5581 acccaggata tccggcgggg tgaagtgacc atcgtttttg atcccaaggg ggatgcagat
     5641 ctgctgctgc gggtctgggc tgaagcgcac cgggccgggc ggggggatga gttgtatatc
     5701 tttcaccttg gctggcctga aatttcagcc cgttacaatg cggtgggacg ctttggtcgt
     5761 gtatcggaag tggcttcccg tgttgccggt cagttgtccg gtgaaggtaa ttcggcggct
     5821 ttccgggagt ttgcctggcg ctttgtcaat atcattgccc gcgccctggt ggccctcggt
     5881 gagcgtccag actatatgct gattatgcgg tatgtgaaca acatcgctga cctctacatt
     5941 cgctatgcgg ggaaggtgat ccgtgagcgt ctgcccggac tggagcagat tatcgcgaat
     6001 aaccagagtg ttctcagcga ggacgatgtg cccaggacca tgcagaacca gccggatgcg
     6061 gtgcggatct ggtcgattga aatggcactg agcagtgagg aaggtaaaaa aatatacgat
     6121 ccgattctgg atggactgcg cagtgctgtc cgctatgacc gtacgtattt tgacaaaatc
     6181 gtggcttccc tgctgccgtt actggaaaaa ctcaccaccg gcaggattgc agagctgctg
     6241 gcgccggact atctggatat gagcgatgcg cgccccattt ttgactggga acaggtgatc
     6301 cgcaaaaagg cggtggttta tatcggcctc gatgcgctca gtgacagtga ggtggccagt
     6361 gccgtgggaa attcgatgtt tgcggacctg gtgagtgtgg cagggcatat atacaaacac
     6421 ggtatgcatg ccggtctgcc ggctggcggg gcgggtaaaa atgtggtgaa cctgcactgt
     6481 gatgagttta acgaactgat gggcgatgaa ttcattccgc tcatcaacaa ggggggcggc
     6541 gccggaatgc aggtgaccgc gtacacccag acgtcatcgg atattgaagc gcgtattggt
     6601 aatgcggcca aaacggcgca ggttcagggg aactttaaca acatgatcat gctgcgcgtc
     6661 agggagaacc ggacggcgga attgctgacc acacaactgc cccaggtgga gatttacacg
     6721 aagacgctgg tctccggtca tcaggatacg gcagatgtgt cagccggtca ggatttcacc
     6781 tccagtaccc aggatcgcgt cgggacggtg aaagtccccc tgctggagcc cgcagatatt
     6841 gtcacgctgc ccaaggggca ggcatttgcc ctgctggaag gtggacagtt atggaagatc
     6901 cggatgccgc tgccggcagg tgatacggat gatgtgctga tgccggagag tatttcccgg
     6961 gtggcggagg cgatgcgaca gaattatcac tccggtgaag gatggtggaa tgcaccacct
     7021 tcagtacagg cgatagcgac ggaggaggga ggtgaccatg gcggaagcac gtcgtgatgc
     7081 agaacgcagt ccgcagtccc gccctccccg tcagcatggg ttgctgtaca atctggtgtg
     7141 gggctggcca tgggctctgg tggggctgct cctctcctca ctgttactga gtctgctgat
     7201 tgagtatgca ggtatgacgt ttttctggcc tgatgagggg gcgtcacaca gtcgtgccgt
     7261 catgaataca gagctgggct ggctgtcgac ggagttcacg cgcagcctgc tgctgtccac
     7321 gccgtcagtg acggtaccgg gctggatttc cgtggcgtat cagtggttgt ttgtggaaag
     7381 tggtatgcag tcatggctgc agtcgcagca ggcggtaacg gcggcggggc gtagtgtgtc
     7441 gcagttcagc ggagagctga gccagtggct ggcatatcat cttcatgatt acctgctggc
     7501 aacggtttat gtgacggtgg tgacgatggt cagggtgatt attctcgcgc tgtccgtgcc
     7561 actgttcgtg atggtgatcc tggtggccgt ggttgagggg ctgggcagac gtgatctgcg
     7621 ccggtatggt gccgcgtatg aatccagctt tgtttatcac catgcgaaaa agtccattaa
     7681 accggcgata tatattccct gtattctcta tctttcctgg ccgacggccg tgtacccgaa
     7741 tctgctgata tggccggcgg ccatgctgct gggtatggct gtcaccgtga caatggcatc
     7801 cttcaagaaa tatttataat ccacagaggc cggacaccct gatgcaaaac ttgttttgtg
     7861 gcagggtgtc ggcagacaag ccacgccctt acggtcagtg cgcgtcagtg tccgggtaat
     7921 gccagcgccg gaataacaac aatgttgacg aaaacaatga acatcgtgat ggccttgacg
     7981 acgtcataga tccacggggg gcaacgtggt tcttcataaa tgaaaaaata cacaactgcg
     8041 tattccacaa ccgtgatcga tttagatgcc aaaccatgat cgattcagat gccattttgc
     8101 aacacaacac aaacccaggt cagaagaaac agggcccaga acggaagagc ggtgcggaga
     8161 aattccatgc tgatgtgttt cctcttttta ttgttaatga ggtgtcatta acgggaaact
     8221 gtagtgatgc cgtgttttaa taaacaggaa taaatcattc aggatggaat gaatttattt
     8281 gaacaggcct ggaacagcgt gatgcagaaa ctggcaatgc tgaccgcccc tttatccacc
     8341 cccgtgtatt ctcccgtaaa aattttcagt tttgcaaata gagtttatcg ctgatccctc
     8401 cccctgcctt acgcatgctg tacgcaccca ttgattctga cggaggtgcg tatgttttct
     8461 cttcctgcat tatcccggaa aacggccggt ctgacgggga ccgccgggat gatgcttctc
     8521 gtgaccctgg ttctgcccgt tcaggccgct gaaaaggatg agctggcgac ggtcatgcgc
     8581 cagcttgatc aggtccgtgc ggcgcttgat cgggccagaa cggcgtccct tcaggccacg
     8641 ccccacgacg ggcgttattt tttcgactat ccgcgtgctc aggctgacat tcagaccatg
     8701 caggagggga tcgaccgtta tctcacgccc tcccgggcgc aaccacgtaa cggcagtgcc
     8761 gtgaccggtg aataccggcg ggagcgtccc tgatggcact gacgtcggca caggctgcgg
     8821 cctttaaggt ggcatccgga gatgtggatc tgaacgtgct gcatctgtcc ttgctgggat
     8881 tactgattgc cgttctcttt ctctgggctg cctggggact gtcggatgtc tggtctggct
     8941 ggcgaaacgg tgacgtcagg gagtctgcgt ttatctggtt tactgcccgc actgttctgc
     9001 tgctggttgc cagcatctgg atgtttgcgg gttgattcat ggtgtgactt tttaaggaga
     9061 aatctatgcg taaaaaaggg cttactgtca gccgtctgcg cctgatggcg cgtctggcgg
     9121 gaatggccat ggtgacgttt ctgctgggca gtctgacacc ggcactggcc gatctgccgc
     9181 agattgaacc gccgtcgtcc ggtggtggtg gcggactttt cgggcaaatc aaaggttatt
     9241 tccaggatgg cattgtgctg attggtctgg tgatcgccgc ggctgccttt gtgaaagtgg
     9301 cggaggcggc cgttaccacg ttttcagagg tgcgggacgg caa
//
LOCUS       DAZFQD010000181         8991 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.181, whole genome shotgun sequence.
ACCESSION   DAZFQD010000181 DAZFQD010000000
VERSION     DAZFQD010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8991
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.181"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            77..1468
                     /gene="xanP"
                     /locus_tag="ACHKTW_004638"
     CDS             77..1468
                     /gene="xanP"
                     /locus_tag="ACHKTW_004638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HFO6465536.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            1589..3298
                     /locus_tag="ACHKTW_004639"
     CDS             1589..3298
                     /locus_tag="ACHKTW_004639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFO6465537.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPATLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPKAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINTEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     PLTGDGNIQLTASGDIQANAPLKPTVSGQLHAVNAAKQQVTQTMNAGIVSSGEVTSAE
                     LVQ"
     gene            complement(3351..5669)
                     /gene="yicI"
                     /locus_tag="ACHKTW_004640"
     CDS             complement(3351..5669)
                     /gene="yicI"
                     /locus_tag="ACHKTW_004640"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HFO6465538.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQDNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPAWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPGLAIYDFTNP
                     DACKWYADKLKGLVAMGVDCFKTDFGERIPTDVQWFDGSDPQKMHNHYAYIYNELVWN
                     VLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFW
                     SHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLK
                     CRMMPYLYREAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGDNVMVAPVFTEAG
                     DVQFYLPEGRWTHLWHNDELDGSRWHKQQHGFLSLPVYVRDNTLLALGNNDQRPDYVW
                     HEGTAFHLFNLQDGHEAVCEVPAADGSVIFTLKAARTGNTITVTGAGEAKNWTLCLRN
                     VVKVNGLQDGSQAESEQGLVVKPQGNALTITL"
     gene            complement(5679..7061)
                     /locus_tag="ACHKTW_004641"
     CDS             complement(5679..7061)
                     /locus_tag="ACHKTW_004641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
                     /protein_id="HFO6465539.1"
                     /translation="MKSEVLSVKEKIGYGMGDAASHIIFDNVMLYMMFFYTDIFGIPA
                     GFVGTMFLVARALDAISDPCMGLLADRTRSRWGKFRPWVLFGALPFGIVCVLAYSTPD
                     LSMNGKMIYAAITYTLLTLLYTVVNIPYCALGGVITNDPTQRISLQSWRFVLATAGGM
                     LSTVLMMPLVNLIGGDNKPLGFQGGIAVLSVVAFMMLAFCFFTTKERVEAPPTTTSMR
                     EDLRDIWQNDQWRIVGLLTIFNILAVCVRGGAMMYYVTWILGTPEVFVAFLTTYCVGN
                     LIGSALAKPLTDWKCKVTIFWWTNALLAVISLAMFFVPMQASITMFVFIFVIGVLHQL
                     VTPIQWVMMSDTVDYGEWCNGKRLTGISFAGTLFVLKLGLAFGGALIGWMLAYGGYDA
                     AEKAQNSATISIIIALFTIVPAICYLLSAIIAKRYYSLTTHNLKTVMEQLAQGKRRCQ
                     QQFTSQEVQN"
     gene            7354..7448
                     /locus_tag="ACHKTW_004642"
     tRNA            7354..7448
                     /locus_tag="ACHKTW_004642"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7388..7390,aa:Sec,seq:tca)
     gene            7748..8932
                     /locus_tag="ACHKTW_004643"
     CDS             7748..8932
                     /locus_tag="ACHKTW_004643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO6465540.1"
                     /translation="MALTDIKVKTAKPKDKPYKLADGGGMYLLINTNGSKYWRMKYRF
                     AGKEKMLSIGVYPDVTLADAREKRSEARKLLAAGGDPGEAKKEEKIAQQISLKNTFEA
                     IAREWHQSKADRWSLRYRDEIIDTFEKDIFPYIGKRPIAEIKPMELLEALRKMEKRGA
                     LEKMRKVRQRCGEVFRYAIVTGRADYNPAPDLASALATPKKVHFPFLTANELPHFLND
                     LAGYTGSIITKTATQIIMLTGVRTQELRFARWEDIDFETKLWEIPAEVMKMKRPHIVP
                     LSEQVIMLFKQLEPISKHHPLVFIGRNDPRKPISKESINQVIELLGYKGRLTGHGFRH
                     TMSTILHEQGFNSAWIEMQLAHVDKNSIRGTYNHALYLDGRREMMQWYADYIDSLSSR
                     ES"
BASE COUNT         2248 a         2330 c         2233 g         2180 t
ORIGIN      
        1 tgaggatgtg gcaaacgttt gcttttctct ttcggtcagc taaaatgccc gctttgctac
       61 cacgggattg ttttcgatgt ctgtttccac cctcgagtca gaaaatgcgc aaccggttgc
      121 gcagactcaa aacagcgaac tgatttaccg tcttgaagat cgtccgccgc ttcctcaaac
      181 cctgtttgcc gcctgtcagc atctgctggc gatgttcgtt gcggtgatca cgccagcgct
      241 attaatctgc caggcgctgg gtttaccggc acaagacacg caacacatta ttagcatgtc
      301 gttgtttgcc tccggtgtgg catcgattat tcaaattaag gcctggggtc cggttggctc
      361 cgggctgttg tctattcagg gcaccagctt caactttgtt gccccgctga ttatgggcgg
      421 taccgcgctg aaaaccggtg gtgctgatgt tcctaccatg atggcggctt tgttcggcac
      481 gttgatgctg gcaagttgca ccgagatggt gatctcccgc gttctgcatc tggcgcgccg
      541 cattattacg ccgctggttt ctggcgttgt ggtgatgatt atcggcctgt cgctaattca
      601 ggttgggtta acgtccattg gcggcggtta cgcagccatg agcgataaca ccttcggcgc
      661 accgaaaaat ctgctgctgg caggcgtggt cttagcctta attatcctgc ttaaccgtca
      721 acgtaaccct tacttacgcg tggcctcact ggtgattgcg atggcggccg gatatgcgct
      781 ggcgtggttt atgggcatgt tgccagaaag caacgaaccg atgacgcaag aactgattat
      841 ggtgccaacg ccgctctatt acggtcttgg cattgaatgg agtctgctgc tgccgctgat
      901 gctggtcttt atgatcactt cgctggaaac cattggcgat atcacggcga cctctgacgt
      961 ttccgaacag ccagtgtccg gtccgctgta catgaaacgc ctgaaaggcg gcgtgctggc
     1021 aaacggcctg aactcgtttg tttcggcggt gtttaacacc ttcccgaact cctgcttcgg
     1081 gcaaaacaac ggagtgatcc agttgactgg tgttgccagc cgctatgtcg gttttgtcgt
     1141 cgcgctgatg ttgatcgtgc tgggtctgtt cccggcagtg agcggttttg tacaacacat
     1201 tccagaaccg gttctgggcg gcgcaacgct tgtaatgttt ggcactatcg ccgcctccgg
     1261 tgtgcgtatc gtttctcgtg aaccgctgaa ccgtcgggcg attctgatta tcgcgctgtc
     1321 gctggcggtt ggtctgggcg tgtctcagca gccgctgatt ttgcagtttg cccctgaatg
     1381 gctgaaaaac ctgctctcct ccgggatcgc cgcgggcggt attactgcca tcgtgctgaa
     1441 tctgattttc ccaccagaaa aacagtaatt cactggcggc ggtaagataa ttaccgccgc
     1501 agtctcacct ctttcaccat tcccgccttg aggattgcgc gtaaatcgtg cataactccc
     1561 ttatgtgcat ttcacgggat ggaagaccat gaaatttatt gggaagctgc ttctctacat
     1621 tctcatcgct ctgttagtgg tgatcgctgg cctctatttt cttctgcaaa cccgctgggg
     1681 agcagaacat atcagcgcat gggtttccga gaatagcgac tatcatctgg ccttcggggc
     1741 gatggatcac cgtttttccg cgccatctca tatcgtgctg gagaacgtca cgtttggccg
     1801 tgatggccag cccgcgaccc tggtggcaaa aagtgtcgac attgcgctaa gcagtcggca
     1861 actgaccgaa ccacgccatg tcgataccat cctgctggaa aacgggacgc tgaatctcac
     1921 cgaccagacc gcgccgctac cgttcaaagc cgatcgtctg caactgcgtg atatggcgtt
     1981 taatagcccg aatagcgaat ggaaactgag cgcgcagcgg gtaaatggcg gcgtagttcc
     2041 gtggtcacca aaagccggta aagtgctggg tacgaaggcg cagattcagt ttagtgccgg
     2101 atcgctttcg ctcaatgatg ttcctgccac caatgtactg attgaaggca gtattgataa
     2161 cgatcgcgtt acgctgacta acctgggtgc cgacatcgcc cgcgggacat taaccggaaa
     2221 cgcgcagcgt aacgccgacg gcagctggca agtggaaaac ctgcgcatgg cggatatacg
     2281 tctacaaagc gaaaaatcgc taaccgactt ctttgcgcca ttacgctctg tcccgtcgtt
     2341 gcagattggt cgcctggaag tgatcgatgc tcgtttgcaa ggtccggact gggcggtgac
     2401 cgacctcgat ctcagcttgc gcaacatgac cttcagtaaa gatgactggc agacacagga
     2461 aggcaaactg tcgatgaacg ctagcgagtt catttatggt tcgctgcatt tatttgaccc
     2521 gattataaac acggaatttt ccccgcaggg cgtagcgctg cgccagttca ccagccgctg
     2581 ggaagggggt atggtcagaa cgtcagggaa ctggctgcgt gacgggaaaa cgttgatcct
     2641 tgatgatgcg gcaattgccg ggctggaata taccttgccg aaaaactggc aacagttgtg
     2701 gatggaaacg acacccggtt ggttaaacag cctgcaactg aagagattta gcgccagccg
     2761 caatctgatc attgatatcg accctgactt cccgtggcag ctcaccgcgc tcgatggtta
     2821 cggtgccaac ctgacgctgg ttaccgatca taaatggggc gtctggagtg gctcggcgaa
     2881 tctgaatgcc gccgccgcga cattcaatcg tgttgatgtt cgtcgcccgt cgctggcgct
     2941 gaccgccaac agcagcacgg tgaatatcag cgaactgagt gcatttactg aaaaaggcat
     3001 tctggaagcc actgccagtg tttcacaaac gccacaacgt cagacccata tcagcctgaa
     3061 tggacgcggt gtgccggtaa atattttgca acaatgggga tggcctgaat taccgttgac
     3121 tggcgacggc aatattcagc ttaccgccag tggagatatt caggccaatg ccccgctgaa
     3181 acctacggtt agcgggcaat tgcatgccgt gaacgccgca aagcagcaag tgactcaaac
     3241 catgaatgct ggcatcgttt ccagcggtga agttacgtcg gcagagctgg tgcagtaaac
     3301 atcaatctcg ccgggtacga ctcagacgta cccggcattc catcaataga ttacaacgta
     3361 attgtcagcg cattcccttg aggcttcacc accagcccct gctcactttc agcctgcgaa
     3421 ccgtcttgca gaccatttac tttcacaaca ttgcgcaggc acagtgtcca gttcttcgcc
     3481 tcgcccgcac cagtcacagt aatcgtgttg ccagtacgtg ctgcttttaa agtaaagatc
     3541 accgatccgt cagcagcggg cacttcacag acggcttcat gcccgtcttg cagattaaag
     3601 aggtggaatg ccgtgccttc gtgccacacg taatcgggac gttgatcgtt gttgcccagc
     3661 gccagtagag tgttatcacg cacataaacg ggcagactca ggaagccgtg ctgctgttta
     3721 tgccagcgac taccgtcgag ttcatcgttg tgccacaggt gtgtccagcg accttccggc
     3781 aggtagaact gcacatcgcc cgcttcagtg aacaccggcg caaccatcac gttgtcgcct
     3841 aacatgtatt gacggtcaag gtaatcacaa gccggatcgt ccgggaactc catcatcatg
     3901 gcccgcatca tcggcgtacc ccgcgcgttc gcacgcgcag cttcacgata cagatacggc
     3961 atcatgcggc atttcagttg cgtgaagaag cgcaccacat cacaggactc atcatcgtaa
     4021 gcccacggca cacgataaga tttgctaccg tgtaaacggc tatggctgga gagcaaacca
     4081 aacgcgcacc agcgtttgta aacgtgcgcc ggagcggtat tttcaaagcc gccgatatcg
     4141 tggctccaga agccaaaacc tgaaaggcca atagacaaac caccgcgcag gctttccgcc
     4201 attgattcgt agttagcgta acaatcgcca ccccagtgta ccgggaattt ctgcgcaccg
     4261 acggaggccg agcgggcaaa caagacagct tcttcctcac caacggtgtc cttgagcacg
     4321 ttccacacca gttcgttgta gatgtacgca taatggttat gcattttctg cggatcggaa
     4381 ccgtcaaacc actgaacatc agttgggata cgttcgccaa agtcggtctt aaagcaatca
     4441 acgcccatcg cgaccagacc tttcagtttg tcggcgtacc atttgcaggc atccggattg
     4501 gtaaagtcat aaatcgccag acctggctgc catttatccc actgccatag cgaaccgtcg
     4561 gggcgtttga gtaaatagcc tttctcttgt aactctttaa agacggggga tttttgaccg
     4621 atataggggt taatccagac gcagattttc agccctttcg ctttcaggcg gcggatcatc
     4681 ccttccgggt cagggaaagt cagcgggtcc cactcaaaat cgcaccactg gaaggctttc
     4741 atccagaaac agtcaaagtg gaaaacatgc agcggcagat tgcgttccgc cataccatcg
     4801 ataaagctgt ttaccgtcgc ttcgtcgtag ttggtggtaa atgaagtggt tagccacagg
     4861 ccgaaggacc acgcgggcgg cagcgccgga cgaccagtaa agcgggtata acgatcaagt
     4921 accgctttcg gcgtcgggcc gtcgataaca aagtattcga gatattcact ctcaacgctg
     4981 aactgcactt tggagacttt ctccgatccc acttcaaaag agacacactg gggatgattg
     5041 accagtaccc cataaccacg gttagtcatg tagaacggga tatttttata cgcctgttca
     5101 gtacttgtgc cgccgtcccg gttccaggtc tctaccgtct ggccattgcg caccagggca
     5161 gtaaagcgct ctcccagacc gtaaactgtt tcgccaacgc caagatcaag ccgctcaaac
     5221 atataattgc gttgattatt cgtgtcctgc acgtagccat tatttttcac ctgactaccg
     5281 gtaatacgtt cgccgttgcg cagaaaatcc agtgaccaga actcaccttt gctgacacgc
     5341 gcgcttaagt tgccactttt aaactcagca taacgttctg tgttttcgat tgtgaccttc
     5401 acgtcctgca aaatattgag cggataatga ggaccgttat tcagcgcccc ctgaaaatgc
     5461 tcaatccgca caccgacaat accttcctgt ggggagaaaa agcgcaacgt aaataaaggc
     5521 gtatcaagct gccaggtacg ttcacgcaca tcacgggggg cagcatagac caccatttca
     5581 ttatcctgct gttcaacctc gaacacctga agcgggtgaa tcaaattgag gccaggttga
     5641 atcaaccagt ttccatcgct aattttcatt gccgttcctt agttctgcac ttcttgagag
     5701 gtgaattgtt gctggcaacg gcgtttaccc tgagccagct gttccataac ggttttcaga
     5761 ttgtgcgtcg tgagtgagta gtagcgttta gcgataatcg cgctcagcaa ataacagatc
     5821 gccggaacaa tcgtgaatag cgcaataatg atgctaatcg tggcgctgtt ctgcgctttt
     5881 tctgccgcat catatccgcc ataagccagc atccagccga taagagcgcc gccgaaggcc
     5941 aaccccagtt tgagcacaaa cagcgtgcca gcaaaactga tcccggtcag gcgtttacca
     6001 ttgcaccact cgccgtagtc gacggtatcg gacatcatta cccactggat aggtgtcacc
     6061 agttgatgca acacaccaat cacgaagatg aagacaaaca tagtgatgct ggcctgcatg
     6121 ggaacaaaga acatcgcgag gctaatcact gccagcaggg cgttcgtcca ccagaagata
     6181 gtgactttac atttccagtc ggtcagaggt tttgccagtg cggaaccaat caggttaccc
     6241 acgcaataag tggtgagaaa agcgacaaac acttccggcg tgcccaaaat ccatgtgacg
     6301 taatacatca tcgccccacc gcgtacgcac accgccagga tattgaaaat ggttagtaaa
     6361 ccgacaatcc gccactggtc gttttgccag atatcacgta aatcttcccg catagacgtt
     6421 gttgtaggtg gtgcttcaac gcgttcttta gtggtgaaga aacaaaatgc cagcatcatg
     6481 aatgccacca cggaaaggac cgcgataccg ccctggaaac cgagtggttt attatcaccg
     6541 ccaattaaat taaccagtgg catcatcaga acagtagaaa gcatgcctcc cgcggtcgcc
     6601 agcacaaaac gccaggattg cagcgagata cgctgagtcg ggtcattggt gattacacca
     6661 cccaatgcgc agtaagggat attgacgacg gtataaagta aggtaagtag ggtgtaagta
     6721 attgctgcat agatcatttt gccgttcata ctgagatctg gcgtgctata ggccagtaca
     6781 cagacgatcc cgaatggcag tgcgccaaac agtacccacg gacgaaattt accccagcga
     6841 gagcgcgttc gatcggccaa caaccccatg caaggatcgg aaatcgcatc cagtgcacga
     6901 gcgaccaaaa acatggttcc gacaaaaccg gcaggaatgc caaaaatatc ggtataaaag
     6961 aacatcatat ataacattac gttatcgaaa ataatgtggc tggcggcgtc tcccatgcca
     7021 taaccaattt tctctttaac ggacaacact tcactcttca tctgcttttc cttgatgcta
     7081 taggggtgct gagaccgata acgcattttg taaaccatcc ctgtgagagc gaatattccg
     7141 atatctggtt attaatttat gattcttgtt ttatgtgatc gtggtagcgt taattccgct
     7201 catatatcat tgtaaaatat gggttttata tgaactataa tgctttcgtg ataatacgct
     7261 gcgtgtatta ggcggaaaaa actgatctgg gggatgtaga aactcaagga agtagctata
     7321 atgcgccccg cctccatgta gcaatcgagg cgcggaagat cgtcgtctcc ggtgaggcgg
     7381 ctggacttca aatccagttg gggccgccag cggtcccggg caggttcgac tcctgtgatc
     7441 ttccgccagt ttacctcttc tgaactctcc caatgtcaat aaatctcttc taatcccttg
     7501 tatatcagta tttatttgta tcctgaggtc aacctacttc accccgtatc aagtttcttt
     7561 tgggggtact ttagggggtg ttatttccta ttgaaataca agcaccccca taataggaaa
     7621 aatgtgatac ataacaacgg cttttaacaa catctttgtt atacaagggg tgtattgggg
     7681 gtatcaggtt agttactggc agaaatcccc ctcaggaaca tgaattcatc aaaagccgga
     7741 ggatgtcatg gcattaaccg atatcaaagt gaaaaccgca aaacccaaag acaaacccta
     7801 taagcttgct gatggcggcg gtatgtatct actgattaac actaatggtt ctaaatactg
     7861 gcgcatgaag taccgctttg ctggtaaaga aaagatgctt tctattggcg tataccccga
     7921 tgtaacgcta gccgatgctc gcgagaaacg cagtgaagcc aggaaactcc tcgctgctgg
     7981 aggcgatcca ggggaggcaa aaaaggaaga aaaaatcgcg cagcaaataa gtctgaaaaa
     8041 tacgttcgag gctattgctc gcgaatggca ccagtcaaaa gccgatcggt ggtcattacg
     8101 ctatcgtgat gaaatcattg atacctttga aaaagacatt tttccctaca ttggcaagcg
     8161 ccctattgcc gaaatcaaac caatggagtt actggaagca ctgcgtaaaa tggaaaaacg
     8221 tggagctctg gagaaaatgc gcaaggtccg ccagcgctgt ggtgaggtgt tccgctatgc
     8281 aattgttact ggccgggcag actataaccc agcccccgat cttgccagtg ctttagctac
     8341 accgaaaaaa gtacattttc cctttcttac tgctaatgaa cttccccact tcctcaacga
     8401 tctggcgggt tataccggaa gtataatcac taagacagct actcagatca ttatgttaac
     8461 cggcgtacga acacaagaac tgcgttttgc acgctgggaa gatatcgatt ttgagacaaa
     8521 attgtgggag atcccggcag aagtaatgaa gatgaagcga cctcatattg tgccgctctc
     8581 tgagcaggtt attatgctat ttaagcagct tgaaccaatc tcaaaacatc atcccctagt
     8641 ctttatcggt agaaatgatc cccgcaaacc aattagtaag gaaagcatta atcaagtaat
     8701 tgaattactt ggatataagg gaagactcac agggcacggt ttcagacaca ccatgagtac
     8761 aattctgcat gaacaaggct ttaactctgc ctggattgaa atgcagttag ctcatgtgga
     8821 taaaaattcc atcaggggta cctataatca tgccctgtat ctcgatggcc gccgtgaaat
     8881 gatgcaatgg tacgctgatt acattgattc actctccagc cgggaaagtt aagtcagaat
     8941 tacttttccc tgatgttttc ctcaattaag tattttatcc tctggcctac a
//
LOCUS       DAZFQD010000182         8802 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.182, whole genome shotgun sequence.
ACCESSION   DAZFQD010000182 DAZFQD010000000
VERSION     DAZFQD010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8802
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.182"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(73..951)
                     /gene="glxR"
                     /locus_tag="ACHKTW_004644"
     CDS             complement(73..951)
                     /gene="glxR"
                     /locus_tag="ACHKTW_004644"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HFO6465541.1"
                     /translation="MKLGFIGLGIMGTPMAINLARAGHQLHVTTIGPVADELLSLGAV
                     SVETARQVTEASDIIFIMVPDTPQVEEVLFGENGCTKASLKGKTIVDMSSISPIETKR
                     FARQVNELGGDYLDAPVSGGEIGAREGTLSIMVGGDEAVFERVKPLFELLGKNITLVG
                     GNGDGQTCKVANQIIVALNIEAVSEALLFASKAGADPVRVRQALMGGFASSRILEVHG
                     ERMIKRTFNPGFKIALHQKDLNLALQSAKALALNLPNTATCQELFNTCAANGGSQLDH
                     SALVQALELMANHKLA"
     gene            complement(1051..1832)
                     /gene="hyi"
                     /locus_tag="ACHKTW_004645"
                     /pseudo
     CDS             complement(1051..1832)
                     /gene="hyi"
                     /locus_tag="ACHKTW_004645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
     gene            complement(1845..3626)
                     /gene="gcl"
                     /locus_tag="ACHKTW_004646"
                     /pseudo
     CDS             complement(1845..3626)
                     /gene="gcl"
                     /locus_tag="ACHKTW_004646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0009028 - tartronate-semialdehyde synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
     gene            complement(3716..4531)
                     /gene="allR"
                     /locus_tag="ACHKTW_004647"
     CDS             complement(3716..4531)
                     /gene="allR"
                     /locus_tag="ACHKTW_004647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HFO6465542.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCL
                     ASAIYDDVGSVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHP"
     gene            complement(4609..5091)
                     /gene="allA"
                     /locus_tag="ACHKTW_004648"
     CDS             complement(4609..5091)
                     /gene="allA"
                     /locus_tag="ACHKTW_004648"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HFO6465543.1"
                     /translation="MKLQVLPLSQEAFSAYGDVIETQQRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQPANLPLTIHELERHPLGTQAFIPMKGEVFVVVVALGDDKPDL
                     STLRAFITNGEQGVNYHRNVWHHPLFAWQRVTDFLTIDRGGSDNCDVESIPEQELCFA
                     "
     gene            5321..6247
                     /gene="allS"
                     /locus_tag="ACHKTW_004649"
                     /pseudo
     CDS             5321..6247
                     /gene="allS"
                     /locus_tag="ACHKTW_004649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308592.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
     gene            6316..7410
                     /gene="mnmH"
                     /locus_tag="ACHKTW_004650"
     CDS             6316..7410
                     /gene="mnmH"
                     /locus_tag="ACHKTW_004650"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043828 - tRNA 2-selenouridine synthase
                     activity [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HFO6465544.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMN
                     NDERAAVGTCYKQQGSDAALALGHKLVAGEIRQQRMDAWRAACLQNPQGILCCARGGQ
                     RSHIVQRWLHEAGIDYPLVEGGYKALRQTAIQAIIELSQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQDLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAAQLDTALTTQLTTGSTDGHLAWLVPLLKEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAEWVKAR"
     gene            7526..7891
                     /gene="ylbG"
                     /locus_tag="ACHKTW_004651"
                     /pseudo
     CDS             7526..7891
                     /gene="ylbG"
                     /locus_tag="ACHKTW_004651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014543172.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(8658..8802)
                     /locus_tag="ACHKTW_004652"
                     /pseudo
     CDS             complement(8658..8802)
                     /locus_tag="ACHKTW_004652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308588.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         2254 a         2288 c         2059 g         2201 t
ORIGIN      
        1 tctttggctg ctttattttt taatattgct ggccgattca ttttctgata tcggccattt
       61 ttattgcggg tatcaggcca gtttatggtt agccattaat tccagcgcct gcactaacgc
      121 agagtgatcc aactggctgc caccgtttgc cgcacaggta ttaaataact cctggcaggt
      181 cgcagtgttt ggcaggttca gcgcaagtgc tttcgcactt tgcagtgcca ggttgagatc
      241 tttctggtgc agagcgattt tgaagcccgg attaaaggtg cgtttaatca tacgctcgcc
      301 atgaacttcc agaatacgtg aggaagcaaa gccgcccatc agcgcctggc gcacacgtac
      361 cgggtccgca ccggcttttg aagcaaatag cagggcttca gaaaccgctt caatattgag
      421 cgccacaata atctgatttg ccactttgca ggtttgaccg tcgccgttac cgcccacgag
      481 ggtgatattt ttaccgagca gttcaaacag cggtttaaca cgttcaaata ccgcttcatc
      541 accgccaacc ataatcgaca acgtcccttc acgcgcaccg atttcaccgc cagagactgg
      601 cgcatcgaga taatcgccgc ccagttcatt cacctgacga gcgaaacgct tagtttcaat
      661 cggggaaatg gagctcatat caacaatggt tttgcccttc agcgaggctt tggtacaacc
      721 attttcaccg aacagaactt cttcaacctg tggtgtatct ggcaccataa taaaaatgat
      781 gtccgatgct tccgttacct ggcgcgcagt ttcaacactg acggcaccca gtgacagtaa
      841 ttcatcagca accggtccaa tggttgtgac atgcagttga tgaccggcac gcgccagatt
      901 aatggccatc ggtgtaccca taatgcctaa gccaataaat cccagtttca taattaacct
      961 cttttaaatt tcgcttttcc tgaattcaga caacacgatc ccctgccaac ttagcggcct
     1021 aaaaaagcat tgcctgaaca gcgttacgtt ttaacggtac ggatccatcc agcgtaaacc
     1081 ggcttccgtg gtggtttggg gtttatattc acacccaacc caacccaacc gttgtagtca
     1141 gaattttcga ttaccttaaa gagataatca taattaattt ctccggttcc cggttcgccg
     1201 cgatgcggat tatcggcaat ttgcaggtga ccaattttat cagcccactg agtcatggtg
     1261 ttggttaatt caccttccat ccgctgcatg tgataaatgt catactgaat tttcaaattg
     1321 cagcaaccaa catcatcaat caatttcagc gcctgccgag ttccggtgag atggaaacca
     1381 ggaatatcaa aatggttaat aggttcaatc agtaataaaa tatcttcttt catcagcata
     1441 ttcgcggcat aacgcaggtt ttctacaagc gttgcgtgaa tctgttcact gctgaaacca
     1501 gccggcgttt taccgaccag acagttaatt tttttattac ccagcgcacg ggcataacga
     1561 atcgctgctg ctacgccatc ccgaaactct tcttcacggc caggaataca ggcaataccg
     1621 cgctcccccg ccgcccagtc acccgccggt aaattgtgca gcgtatgttc gagtttatta
     1681 ctcgccagca cctgttttaa ttcttcaatg tcgtagtcat aaggaaacat aaattcaacg
     1741 ccgcgaaaac cacactgcgc agctttctca aaacgggcga gaaaatcata ttctccaaat
     1801 aacatcgata aattagcaga gaaacgtaac attatttatc tcccttattc atagtgcatg
     1861 aagcaggttt cagtcggtgc gtccgctgcg ttatcggcga tatcttcaaa ttccatgacg
     1921 ttatccagtt cgctgcccat cgaaatattg gtcacacgct cgagaataac ttccacgact
     1981 accggtaccc gatattgcgc cattaaggct ttcgcctgtt caaaggctgg cgcaatatct
     2041 tccggtttga agacgcgaat cgctttacaa cctaaacctt ccgctacttt tacgtggtca
     2101 acagcgtagc cattcacttc actggagttg atattctcga aagcgagttg cacgcagtag
     2161 tccatatcaa acgcgcgctg cgactggcga atcagcccca gataagcgtt attgaccagt
     2221 acatggatgt acggaatgtt gaactgtgcg ccaacggcca attcttcaat caggaactgg
     2281 aagtcgaagt caccggaaat cgccaccact ttgcgctctg gatcagcggc acaaaccccc
     2341 agcgccgccg gaatggtcca gcccagcggg cctgcctgcc cacagttgat ccagtggcgg
     2401 tctttaaaga catgcagcat ttgcgcagcg gcaatttgcg acagaccaat ggtggtgaca
     2461 tagcaaacat cgcgaccaaa ggctttgttc atctcttcat acacgcgctg cggtttcacc
     2521 ggcacgttgt cgaaatgggt tttgcgcagc aaagtgcgtt tgcgctgctg gcagtcggcg
     2581 acccattctt tacgacacgg cagacgaccc gctttttgca tctcctgcgc cacttcaacc
     2641 agcagtgtca gcgccgcttt agcatcagag acaatgccga gatccggaca cagcacgcga
     2701 ccaatttgcg tcggctcaat atcgatatga acgattttgc gcccttcggt gtatttctct
     2761 accgaaccgg tatgacggtt agcaaaacgg ttaccgatac caaacaccat gtcggacgcc
     2821 agcaacgttg cgttaccgta acgatgcgcg gtttgcagac ccaccatccc ggccatcagt
     2881 tcatgatcgt ccgggataca gccccagccc atcagcgttg ggatcaccgg aacgctggtc
     2941 agttcagcaa actgttgtaa cagtgcagct gcgtcagcat taattacccc gcccccggca
     3001 acaatcaccg gacgttcggc ctagattaac atttctacag ctttttcgat ctgcatacgg
     3061 ctggcagcag gtttgtagac cggcagcggt tcgtacatgt caggatcaaa ctcgatttcc
     3121 gcaacctgaa cgtcgaacgg taaatccacc agtaccggac ccggacgacc agaacgcatc
     3181 aggtgaaatg cctgttgcag cacacgaggc accagcgccg cttcacgtac tgtaaccgcc
     3241 attttgctga ccggcttggc gatagcttcg atatcaacag cctggaaatc ttctttatgc
     3301 agacgggcgc gcggagcctg gccggtaata cacagaatag gaatggaatc agcagaagca
     3361 gaatagagcg cggtgatcat gtccgtgccc gcaggaccgg aagtccccag acatacgccg
     3421 atattccctg ccgttgcgcg ggtataacct tccgccatgt gcgaagcacc ttccacatga
     3481 cgcgccagaa tgtgacgaat accgccgtgc ttacgcatcg ctgagtagaa cggatttatt
     3541 gcagctcccg gaacgccgaa ggcggtggtg ataccttctt tctccagcac atacattgcc
     3601 gcgtcaacgg ctctcatttt tgccatttta ttcctacctc tattaattgg aaaatttttc
     3661 caactttcaa attttgtcaa aaaaaaccgc tccgcagagc gggatgcgac agacattatg
     3721 gatgtgcttt cagtcccaac gccgtgctga tatcgcgggc ggtgtctctg accagctcac
     3781 cctgactgac aaaacgatct tctgtcagtc ttgatgaagg cccggagatg gagatagcgg
     3841 caacaacact acctacatca tcgtaaattg ctgaagctaa gcaattcaga cctacaacat
     3901 gctcttcttt atctacggta tagcccagtt cacgcgcttg ttccaggtcc ttcagcaagg
     3961 tgggcatatc cacaagcgta gttggcgtaa actgctgcaa accggtttgc agaatgatgc
     4021 tcatcaactc ctcttccgcc agcggataaa gcagcgcttt gcccgcaccg gaagcatgca
     4081 gcggcagacg actgcccagt ggcgcacaca tcctgaccat tgatttacac tctaactgac
     4141 caattaatac tgcttcattg ccgttacgga ttgcgacatt gaccgtttcg ccggaaagta
     4201 acatcaggcg gcgcataaac ggcccggcga cggagaggac atcgcggtta tggatgtacg
     4261 ccgcaccgac gttaaagaca cctaatccta tatgccacca gcctaactga ctgtcctgat
     4321 agacaaaatc cgctgcctgt aaaaccttca gcaagcgaaa ggtcgtggag agcggcaaat
     4381 ccagattgag agaaatatcg ctaaccgacg agcttccccc acttttttcc agatattgca
     4441 gaatcgcaat tccccgctct aacgcctgtg cgcccttctg tgccacaggc tccgcctgtc
     4501 ctggcctgcc gcgccgtcta acttccgtca tacctacgtt aactccgacc tacaagtgag
     4561 cataaacaat gaacaattag tttatcttat gcaagtcggt tgcaggcgtc acgcaaaaca
     4621 gagttcctgt tcaggaatac tttcaacatc acagttgtca ctgccgccgc gatcgatggt
     4681 cagaaaatcg gtgacgcgct gccaggcgaa aagtgggtga tgccagacgt tacgatggta
     4741 attcactccc tgttcgccgt tggtgataaa cgcccgcagc gttgacaggt ctggtttgtc
     4801 gtcgcctaac gccacgacca ccacaaacac ttcacctttc atcgggataa aggcctgagt
     4861 gcccagcgga tgacgttcga gttcgtgaat ggtcagcggc agattcgccg gttgcgcgcg
     4921 gttaatgctg ataagcgtac ggtcttgctc aagaatctca accagcgcca aatcgtggta
     4981 acgctccacc aggccattgt taatatggaa aaaatcccgt tgctgagttt cgattacgtc
     5041 gccataagca ctaaaggctt cctgacttaa cggtaatacc tgaagtttca tctttttgtc
     5101 cttgtaacat aattccgttc aaccatcgcc gaccggtcag cttggtttca ataacaggca
     5161 atctaaaacc ttaagagaac aacatcaata caaaagttgg aatatttttc caaaactaga
     5221 tttagtccac aaattgaaca gggttatcat gtaaaaatac tgataaatca gaggataaga
     5281 aaatcacgca acttgcggat ttaaaagggg atacctgaag atgttcgatc cagaaacctt
     5341 gcggactttc attgcggttg ctgaaacagg aagtttttca aaagcggcag aacgattatg
     5401 taaaaccacg gcgacgatca gttatcgcat taaacttctg gaagagaata ccggagtagc
     5461 gctgtttttc cgcacgactc gcagcgtgac gttgacagcg gctggcgagc atctactttc
     5521 ccaggccaga gactggctga gctggctgga aagtatgcca agcgagctgc aacaggtgaa
     5581 tgatggcgtg gaacgctagg tgaatattgt catcaacaac ctgctctacc acccccaggc
     5641 cgtcgcccag ttgctggcgt ggctgaatga acgttacccc tttacccagt ttcacatctc
     5701 ccgacaaatc tatatgggcg tctgggattc gctattatac gaaggttttt cactggctat
     5761 cggcgtcacg ggaaccgagg cgctggcaaa tacctttagt cttgatccct taggatcggt
     5821 gcaatggcgc tttgtcatgg cggcggatca tccgctggcg aacgttgaag agccgctaac
     5881 agaagcgcag ttgcggcgct ttccggcggt caatattgaa gacagcgccc gcaccttaac
     5941 caaacgcgtc gcctggcgat tgccagggca aaaagagatt attgttcctg atatggaaac
     6001 gaaaatcgcc gcccatcttg cgggcgttgg cattggtttt ttgccaaaat cgctttgcca
     6061 gtcaatgatc gataatcaac aactggttag ccgggtaatc ccaacgatgc gccctccttc
     6121 gccattgagt ctggcatggc gcaaatttgg cagcggcaaa gcggtagaag atattgtgac
     6181 cttgtttacc cagcgcaggc cggaaatcag cggattttta gaaattttcg gcaacccacg
     6241 cagttaaaaa tcaagtttcc ttgcgcgttg gcgcgtaaaa tagcgcgcaa gctgatttga
     6301 ggttcacttt taactatgca agagagacac acggaacagg actatcgtgc cctgctgatt
     6361 gctgatacgc ccattattga tgttcgcgcc cctatcgagt ttgagcaagg cgcaatgccc
     6421 gccgctatca atctgccgtt aatgaataac gatgaacgcg ccgccgttgg cacctgctat
     6481 aaacagcaag gctcagacgc agcgctggcg ctggggcata aactggtggc gggtgaaatt
     6541 cgtcagcagc gcatggacgc ctggcgggca gcgtgcctgc aaaatccgca aggtattctc
     6601 tgctgcgccc gtggcggtca acgttcacat attgtgcaac gctggctgca tgaagcgggg
     6661 attgattatc cgctggtgga aggcggttat aaggcactgc gccagaccgc gattcaggcg
     6721 atcattgaac tgtcacaaaa accgatagtg ctgattggcg gttgtaccgg cagcggtaaa
     6781 acgctattag tgcaacaaca gccaaacggt gttgatctgg aagggctggc gcgtcatcgc
     6841 ggttcggcgt ttggtcgcac gttacaacca caacttagcc aggcgagctt tgaaaacctg
     6901 ctggctgccg aaatgctaaa aaccgacgcc cgtcaggatt tgcgcctgtg ggtgctggaa
     6961 gatgaaagcc ggatgatcgg ttcgaatcac ctgccggaat gtctgcgcga gcggatgact
     7021 caggcggcga ttgcggtggt agaagatccg tttgagatcc gtcttgaacg cctgaacgaa
     7081 gagtatttct tgcgtatgca tcatgatttt acccacgctt atggcgacga acagggctgg
     7141 caggaatatt gcgaatacct gcatcacgga ctttcggcga ttaagcgtcg gctggggtta
     7201 cagcgctata acgaactcgc tgcccagctg gatacggcgc tgacaacgca actcaccacc
     7261 ggcagcaccg acggtcatct ggcctggctg gtgccgttac ttaaagaata ttacgaccca
     7321 atgtatcgct atcagcttga gaaaaaagcg gaaaaagttg tctttcgcgg tgagtgggcg
     7381 gaagtggcgg aatgggttaa ggcgcggtaa tgagggacgt atttgtatta gtcgcgactt
     7441 gatctgacac atggccttga aaggttgaga gttaccggtt ttgatatgag tgtcgaatcc
     7501 ttatccaaaa caagaggtaa ctctcatgct tcatactgcc aatcccgtca tcaaacacaa
     7561 agccggtttg ctcaatctgg ctgaagaact cagcaacgta tcaaaagcct gtaaaatcat
     7621 gggcgtttcg cgtgatacgt tttatcgtta ccgcgaactg gtcgctgaag gcggtgtgga
     7681 tgcgcagatt aatcgtagtc gccgcgctcc taaccttaag aaccgtaccg atgaggcaac
     7741 tgaacaggct gttgttgatt acgccgtcgc tttcccgaca catggtcagc accgggccag
     7801 caacgagctg cgtaaacagg ggggttttat ctccgactgt ggtgtccgtt ctgtctggtt
     7861 actccataac cttgagaatc tcaaaagacg atactgaaca aattttatca ggtgacgttc
     7921 cgtaaaaagt tgtatggagt tctcgatgca gtcggcgcga ctatcagtac ccccgctgaa
     7981 ccctggacta tcgggccgcc gtactggggc atgtcaccct ggcgtgccgc tggttttccg
     8041 ctaatggttt atttcctctt tctttcccgg caagaattta ttttaatccg gaaggattat
     8101 ttattttcag atagggactc acaataaaca cgtaacattt taattaatgc atggctgaat
     8161 atgtattaca caatgtcaga tatcgtaaaa gaccagtgca attctatgta aactctctga
     8221 ctgttcattt tatttgttgt ttcagggtcg gaactcaccc tcaataaaca actgaatacg
     8281 agtagatatt catcgtctga gctatatggc tttacacaat agccgacatg tcttttctca
     8341 gtcaggaatt ttacttttcc tcactacgaa gttgacgact ttgatatggg atagactctt
     8401 aattcaagca attactctgc acaagcatca ggcaatccag ctccaatatt tgttataaaa
     8461 taatgcgtaa cattaatttc accaacaaaa aaaaggttta caccatcccc ttgccctggt
     8521 atcttaccat atttacacca cacttttttt gtatctggat cattctttga tacgattttc
     8581 gccacttgtt ccctggccct cactgtcaat gttttcttaa acctaccccc atcatcattt
     8641 gttagggaaa aataagaaaa aaaataatca tcttctacat ttgtgtatat tgatatatcc
     8701 accgctacgt gcggaaaaaa atctgggagc catgtaaaaa ctccatcatt ttctggtatt
     8761 gccatatagt ttatgtaatg tttacttaca agagattcat ct
//
LOCUS       DAZFQD010000183         8739 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.183, whole genome shotgun sequence.
ACCESSION   DAZFQD010000183 DAZFQD010000000
VERSION     DAZFQD010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8739
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.183"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..429
                     /locus_tag="ACHKTW_004653"
     CDS             <1..429
                     /locus_tag="ACHKTW_004653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000062365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO6465545.1"
                     /translation="AAPRTYHSFEPVNEPKNIMFEHVRTEWEKTPDRHEETDKAFEEI
                     FWCAGMRKAGKKNAASAFRTQFREWRKTTRGTASEFATMLAEDIACRNGKQFGFDRLL
                     PSSYLNGQRWNDEKPETIQPQSKPSSAITVSKTGYVFFDR"
     gene            540..2420
                     /locus_tag="ACHKTW_004654"
     CDS             540..2420
                     /locus_tag="ACHKTW_004654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HFO6465546.1"
                     /translation="MTPSELSDLLWAQVDRVAPHLLPNGKKEGHEWVAGNVNGDKGNS
                     LKVNLSGKKKWADFAEGDGGDMLDLWMACRGINLHQAMQEAKAFLGIKDDDHHFDARR
                     EKKFSRPDRKKIASYVTRTESHLEYLQSRGISPEVVKRYEVVSGKVWNGERELDALVL
                     PYKRDGELLQVKRISTERPDGKKVIMAEGDCEPCLFGWQALDAGVRAVVLCEGEIDCM
                     SYAQYGISALSVPFGGGKGAKQQWIEFEYHNLDRFEEIFISMDVDDVGREAAREIASR
                     LGEHRCRLVTLPYKDINECLMNGVTEDEIWQYIGTASYFDPEELYSAREFYQDTINAF
                     YGKQQYLFNPPWESLADKFQFREAELTLVNGVNGHGKTEVVGHMALEAMRQGVKTCIA
                     SLELKPGILLKRLTRQATCCKMPPVLEIDSAFKFYDERLWVFGLTGTAKADRLIEIFD
                     YARRRYGIQLFIIDSLMKCGIGDDDYNGQKAFVDSICDFKNKTNSHVILVTHSRKGDS
                     EEKPTGKMDVKGSGAITDLTDNLFIIWRNKARERALQRVQSGEQMSEKDEQLLVSPAS
                     VLLLEKQRNGEGWEGGIPLFLDEQSHQFLQLESGSPYSYIANMPKSEYDEAWRQENVT
                     EY"
     gene            2510..2725
                     /locus_tag="ACHKTW_004655"
     CDS             2510..2725
                     /locus_tag="ACHKTW_004655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6465547.1"
                     /translation="MSTIAELVRANFREELVRWYRYRSSSSLPLDELYEHSPAARRYP
                     RDRVLRRLFKLNNEFQRNRIIRSLDLK"
     gene            2899..3315
                     /locus_tag="ACHKTW_004656"
     CDS             2899..3315
                     /locus_tag="ACHKTW_004656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinB"
                     /protein_id="HFO6465548.1"
                     /translation="MRKLTFELRSPIHQQNAIQAIQQILPDPTKPIVVTIQERNRSIR
                     QNARLHAMLSEISKKATYHGKARNIEFWKGLFVSGWQIATNQHPEIISGLEGELINIR
                     ESTATLSVKKISEIMDYIEAYCAMNSINLSEWRNYD"
     gene            3308..3910
                     /locus_tag="ACHKTW_004657"
     CDS             3308..3910
                     /locus_tag="ACHKTW_004657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000573862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUMOD4 domain-containing protein"
                     /protein_id="HFO6465549.1"
                     /translation="MIEVWVDIEGIPFYQVSNKGNFRSITREITVTSTRQRPYKKIIN
                     GTSVKPFKCKSTGYLQIKVYGKKYSTHRIVAKAFCTGFCDGLVVNHKNGQRDDNRAEN
                     LEWVSHSENSKHGYKQNGRIPISLGKFSGDHPASKAVISTDMKTGEEVYYEAAMDAVR
                     EGFDSSSISRCCNGESSYHKGRFWRFANETMKARWGDRAA"
     gene            3907..3984
                     /locus_tag="ACHKTW_004658"
                     /pseudo
     CDS             3907..3984
                     /locus_tag="ACHKTW_004658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311007.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
     gene            4092..5141
                     /locus_tag="ACHKTW_004659"
     CDS             4092..5141
                     /locus_tag="ACHKTW_004659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000335902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P63C domain-containing protein"
                     /protein_id="HFO6465550.1"
                     /translation="MCIIDLSITTRDMLMKNDDVSGKAKGGKARAAKMTAEQRKESSR
                     KAVAAKKEKALLPVSANEGKLKIGDAELDVAVLENGRRIISQASVFKAFGRPQRGGRA
                     PQEEGVINMPAFMDAANLKKYINQDVMGVINKVKYKTITGSVQEGYDASIIPLVCDVY
                     LKAREAGAITRPNQLETAKKAEILVRSLAKVGIIALVDEATGYQRDREKDALAKILEA
                     FVAKEIQPYITTFPADYYEELFRLRGLEYPPENPRFRPQYFGVLTNDIVYKRLAPNIL
                     EELKKQNVKASKGTKLFQGLTPNIGYQKLREHLSSTVTIMKLSNDYSDFIAKMNRLHP
                     RFEDVKTDELDDSDK"
     gene            5293..6015
                     /locus_tag="ACHKTW_004660"
     CDS             5293..6015
                     /locus_tag="ACHKTW_004660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HFO6465551.1"
                     /translation="MNELINSNVIKMTSIEIAELTGKRHDNVKRTIETLVKSGVIRLP
                     QIEVSERINNLGFNVQYEHYVFEGEKGKRDSIIVVAQLSPEFTARLVDRWRELEGATA
                     KIPQTFSEALRLAADLEDQKAELEKQLALAAPKVEFADRVGEASGILIGNYAKVVGIG
                     PNKLFAWMRDHKILITSGARRNVPMQEYMERGYFTVKETAVNTNHGIQISFTTKITGR
                     GQQWLTRKLLDNGMLKVTGEAA"
     gene            6015..6620
                     /locus_tag="ACHKTW_004661"
     CDS             6015..6620
                     /locus_tag="ACHKTW_004661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinG"
                     /protein_id="HFO6465552.1"
                     /translation="MAKPARRKCKICKEWFHPAFSNQWWCCPEHGTQLALERRSKERE
                     KAEKAAEKKRRREEQKQKDKLKIRKLALKPRSYWIKQAQQAVNAFIRERDRDLPCISC
                     GTLTSAQWDAGHYRTTAAAPQLRFDERNIHKQCVVCNQHKSGNLVPYRVELISRIGQE
                     AVDEIESNHNRHRWTVEECRAIKAEYQQKLKDLRNSRSEAA"
     gene            6664..7182
                     /locus_tag="ACHKTW_004662"
     CDS             6664..7182
                     /locus_tag="ACHKTW_004662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015973887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFO6465553.1"
                     /translation="MGIRELNLTKEQHDWLNGWLELWGAWVYSGRLEKRMSSVIAKFM
                     ESVEPGRVMTRPMCNDDDGMLISQVVDSVMYIDKKAFGILLSYYAHGSSKRAIASYYH
                     ATAKPRKMCGRGGEGWRKPSLATCRNEIDDILKASLFVLYQPMQNAFKMRKRVEKVKH
                     VAVKSLDMQLAI"
     gene            7489..7920
                     /locus_tag="ACHKTW_004663"
     CDS             7489..7920
                     /locus_tag="ACHKTW_004663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance protein"
                     /protein_id="HFO6465554.1"
                     /translation="MFGIFGKKARRAAVEIKKFEKRDLAQAVINAAYLVAYADGECEA
                     SEKAKIEQVLRNQPALSAFTSEINAISATIIGQLDTNFKIGRRAALREIEDVKHDTRE
                     AEDVLDVAVAIAEADGEIEPEERKVLEEIAGVLGLRLENHL"
     gene            7917..8081
                     /locus_tag="ACHKTW_004664"
     CDS             7917..8081
                     /locus_tag="ACHKTW_004664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HFO6465555.1"
                     /translation="MTVKMRLAVVALLLFLVVMVDFSSRIMSVLADGVLVAGVVVVAF
                     PLLKKKASGD"
     gene            complement(8175..8306)
                     /locus_tag="ACHKTW_004665"
                     /pseudo
     CDS             complement(8175..8306)
                     /locus_tag="ACHKTW_004665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311526.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(8377..8712)
                     /locus_tag="ACHKTW_004666"
     CDS             complement(8377..8712)
                     /locus_tag="ACHKTW_004666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HFO6465556.1"
                     /translation="MKWIVIDTVIQPSCGISFSVIWSKIKLIIWYQSDAFLPPESIFT
                     LTHTGIMLNNKVLPVTIYNVVPFNKKFWNLIKNSQECPTNTDNVLNECFNNRCTLQIC
                     PYGLKQQSP"
BASE COUNT         2569 a         1818 c         2252 g         2100 t
ORIGIN      
        1 gctgccccca gaacctatca ctcttttgaa ccagtcaatg aacctaaaaa cattatgttc
       61 gaacatgtcc gaacggagtg ggaaaaaact cctgaccgtc acgaagaaac cgacaaggca
      121 ttcgaggaaa tattctggtg tgcaggcatg cggaaagccg ggaagaaaaa cgcagcttca
      181 gcattcagaa cacagttcag ggaatggcgt aaaactacca ggggtacggc aagcgagttt
      241 gccacgatgc tggcagaaga catcgcatgc aggaatggta agcagttcgg attcgacagg
      301 ttgttaccat cgagctacct gaacggtcaa cgctggaacg acgagaagcc agaaactatt
      361 caaccacaat ccaaaccatc atccgcaatc accgtatcga aaactggcta cgtgtttttc
      421 gacaggtgaa ccatgaaatc cagaatcaaa tcgttactta tcgctggcta taaccatggc
      481 tggcttagtt ctgcattcgt tgagttctgg tttaaccgtc tcgatctgag gtcagcgtaa
      541 tgactccaag tgaactgagc gacctgcttt gggcgcaggt tgacagggtg gctccgcacc
      601 tgttgccaaa cggcaagaaa gaggggcatg agtgggttgc cggtaacgtt aacggtgaca
      661 agggaaacag ccttaaggtc aaccttagcg gcaagaaaaa atgggctgat ttcgctgagg
      721 gagacggcgg tgacatgctt gatttgtgga tggcatgtcg tggaattaac ctgcatcagg
      781 ctatgcagga agcgaaagca tttctcggta tcaaggatga cgatcaccat ttcgatgcca
      841 gacgtgagaa gaaattctcc agacctgatc gcaagaaaat cgccagttac gttaccagaa
      901 cagaatccca tcttgagtac ctgcaatcgc gtggcatatc gccagaagtc gtaaagcgct
      961 acgaggttgt cagcggcaag gtgtggaatg gagagcggga actggatgct ctggtgcttc
     1021 cgtacaaacg cgatggtgag ttgttgcagg tcaagcgaat cagcactgag cgcccggacg
     1081 ggaagaaagt cattatggca gaaggtgatt gcgaaccttg tctgttcgga tggcaggctc
     1141 tggacgctgg cgtgagggcg gttgtacttt gcgaaggcga aattgattgt atgagctatg
     1201 cgcaatacgg catctcggcg ttatccgtgc cgtttggtgg cgggaaaggc gctaagcagc
     1261 agtggattga gtttgagtat cacaacctcg acaggtttga ggaaatattc atctcgatgg
     1321 atgttgatga tgttggtcgt gaagccgcaa gggaaatcgc aagccgactc ggtgaacatc
     1381 gttgccgtct tgttactctg ccgtacaaag acatcaacga atgcctgatg aacggtgtta
     1441 ccgaggatga aatctggcag tacatcggca cggcatccta cttcgatcct gaagaactct
     1501 acagcgcgcg agagttttac caggacacta tcaacgcttt ctacggcaag cagcagtatc
     1561 tgtttaatcc accgtgggaa tctctggcag ataaattcca gttccgtgag gccgagttga
     1621 cgctggtcaa tggtgtgaac ggtcacggaa aaacggaggt tgtcgggcat atggcacttg
     1681 aggcaatgcg tcagggtgtg aagacgtgca tcgcgtcact tgagctgaag cctggtattc
     1741 tccttaagcg ccttacccgt caggcgacgt gctgcaagat gccgccagtg ctggaaattg
     1801 actctgcatt taaattttat gacgaaagac tttgggtgtt tggcctgacc ggaacggcga
     1861 aagccgacag gctgatcgaa atattcgact acgctcgccg ccgatacggt atccagttat
     1921 tcatcatcga cagcctgatg aaatgtggca taggcgacga tgactataac gggcagaagg
     1981 cgtttgttga ctcgatttgc gacttcaaaa acaaaacaaa ctcccacgtc attctcgtta
     2041 ctcactcgcg aaaaggagac agcgaagaaa aaccaaccgg gaaaatggac gtaaaaggct
     2101 ctggagcgat aacagacctg acagacaacc tttttatcat ctggcgtaac aaggctcgcg
     2161 agagagcgtt acagagagtt caaagcggtg aacagatgtc agagaaggac gaacagctac
     2221 tggtgtctcc ggcatctgtt ttgttgcttg aaaaacaacg taacggtgaa ggttgggaag
     2281 gtggtatccc gttgttcctt gacgagcaat cgcaccagtt cctgcaactt gaatcaggat
     2341 ctccatatag ctacatcgcc aatatgccga aatcggaata tgacgaggcg tggagacagg
     2401 aaaacgtgac ggagtattaa atgaccatct acatcactga gcttgtaaca ggcctgctgg
     2461 taatcgcagg cctttttatt tggggaagag taattggagg ctttaagaaa tgagtacgat
     2521 agctgagctt gtcagggcta attttcgtga agagttggtg cgttggtatc ggtatcgttc
     2581 atcgtccagt ttgccgcttg atgagttgta tgagcattca cctgccgcac gacgctatcc
     2641 gcgtgaccgt gttcttcgac ggttgttcaa actcaacaat gagtttcagc gcaacagaat
     2701 tatccggagt ctggatttaa agtgaaggag tgagcatgag cgagcaaata ttcagagaga
     2761 ttaagccacg gttttatcgc aaggtaaggg tagtttatca ggacgaaaac aagacctgtg
     2821 catacgccat tcataatggt cggtggtcag tgttcgacac caaaaacttc gagaagaact
     2881 tcgagaggat taagggtgat gaggaaacta acatttgaac taagaagccc catccatcag
     2941 cagaacgcca ttcaagctat ccagcaaatt cttccagacc caaccaaacc aatcgtagta
     3001 accattcagg aacgcaaccg cagcattcgg caaaatgcac gccttcacgc gatgctatct
     3061 gaaataagta agaaggctac atatcatgga aaagcaagaa atattgagtt ttggaagggg
     3121 ttattcgttt ctggttggca gattgcaacc aaccagcacc ctgagattat atcagggtta
     3181 gaaggtgagc taataaacat cagagagagt acggcgactc tatctgtaaa aaaaatatcc
     3241 gaaataatgg actacataga agcatattgt gccatgaact caattaatct tagcgaatgg
     3301 aggaattatg attgaggttt gggtagatat cgaaggaatt ccattttatc aggttagcaa
     3361 taaaggaaat ttcaggtcta ttacgaggga aattacagta acatcaacca gacagaggcc
     3421 atataagaaa ataattaatg gcactagcgt aaaaccattc aagtgcaagt cgacaggata
     3481 tcttcaaata aaggtatacg gtaagaaata cagcacccac aggatagttg cgaaagcatt
     3541 ctgtacaggg ttctgtgatg gcttggtagt taatcacaaa aatgggcaaa gagatgacaa
     3601 tagggctgaa aaccttgaat gggtatcaca ttctgaaaac tcaaagcacg gatataaaca
     3661 aaatggaaga atacctatat cgctaggtaa atttagtggt gatcatcctg ccagtaaagc
     3721 tgttatttct actgacatga aaactgggga ggaggtttat tatgaagcag ctatggatgc
     3781 tgtcagagaa ggatttgata gttcgtcaat tagtcgttgc tgtaatggcg aaagctcata
     3841 tcacaaagga agattctggc gatttgcaaa tgaaacaatg aaagcaagat ggggagacag
     3901 ggcggcatga ggcgacagcg acgaagtttc accgacatca tctgcgaaaa ctgcaaatac
     3961 cttccaacga aacgctccag aaatttagtt ttgagcagaa taccatgatg tcagtgcaag
     4021 ggggagaaag tctcctcatt atctgattcg caatttacgt gcatatttaa atattgcacg
     4081 ttacaacgtg catgtgtata attgacttat caatcacaac acgagatatg ctcatgaaaa
     4141 atgatgatgt tagtgggaag gccaaaggcg gtaaggcacg cgcggcaaaa atgacagcag
     4201 agcaaagaaa agaatcctca agaaaggctg ttgccgcaaa aaaagaaaaa gctttattgc
     4261 ccgtatctgc gaatgaggga aagttaaaga tcggtgatgc ggaattagat gtcgcggttc
     4321 tcgaaaatgg acggcgtatc atatcacaag cttctgtttt taaagcattt ggccgaccac
     4381 aaagaggggg tagagcacct caagaagagg gggtgatcaa tatgcccgct tttatggatg
     4441 ctgcaaacct taaaaaatat ataaatcaag atgttatggg tgtgatcaat aaggtcaaat
     4501 acaagacgat tactggctcc gtccaggaag gttatgacgc atccataata cctcttgtct
     4561 gcgatgttta tttaaaggca agagaggcag gcgctatcac caggccaaac cagttagaga
     4621 cagccaagaa agctgaaatt ctggtgcgct cattagctaa agtcggaata atagcgcttg
     4681 ttgatgaagc gacggggtac cagcgagata gagaaaaaga tgcgctcgcc aaaatactcg
     4741 aggcctttgt cgcaaaggaa attcaacctt atattacaac atttcctgct gattattatg
     4801 aagagctttt caggttaagg ggcttagaat acccgccgga aaatccccgc ttccggcctc
     4861 agtattttgg cgttttgaca aatgatatcg tctacaagag attggcacca aacatccttg
     4921 aggagcttaa aaagcagaac gtaaaggcct caaaaggtac aaagttgttt caggggctga
     4981 cgccaaatat tggatatcaa aaattaagag agcatctgtc atcaaccgtt acgattatga
     5041 agctatctaa cgactattca gattttattg caaaaatgaa tcgcctgcat ccaaggtttg
     5101 aggatgtgaa aacagacgaa ctggatgatt cagacaagta acagtaaccc accttcaggt
     5161 ggtttttttg tacaaatcct tcagcgtaag tttacctcct tcactgcatt actactgacc
     5221 attgacaact taacaaaccc agcttcggct gggtttttta ttggtgaatt ttcaatatga
     5281 gaggacatga caatgaacga gctgataaat agcaacgtca tcaaaatgac tagcattgaa
     5341 atcgctgagc ttacaggtaa gcgtcatgac aatgtgaaac gaaccatcga aacgctggtt
     5401 aaaagtggag ttatccggct tcctcaaatt gaggtttccg aaagaatcaa taacttaggg
     5461 ttcaatgttc agtacgagca ttacgtcttc gaaggcgaaa aaggtaagcg cgacagcatt
     5521 attgtcgtcg ctcagttgtc gccggaattc accgctcgtc ttgttgaccg ttggcgagaa
     5581 ctcgaagggg caaccgcgaa aataccacaa accttttctg aggcattgcg ccttgcggcc
     5641 gaccttgaag accagaaggc tgaactggag aaacagcttg ctctcgcagc acctaaagtt
     5701 gagtttgccg atcgcgttgg cgaggccagc ggaattttga ttggaaacta tgcaaaggtt
     5761 gttggaattg gtccaaacaa actgtttgcg tggatgcgcg atcacaaaat ccttattact
     5821 tcaggtgccc ggcgcaatgt gccaatgcag gaatatatgg agcgcggcta tttcacagtg
     5881 aaagaaacag cggtcaacac aaatcacgga atacagatat cgttcaccac aaaaatcacc
     5941 gggcgtggtc aacagtggct gaccagaaag ctgctcgata acggaatgct gaaagtaaca
     6001 ggggaggctg cttaatggct aaaccagcgc gaaggaaatg caaaatatgc aaagaatggt
     6061 ttcacccggc attctcaaat cagtggtggt gctgcccgga acacggaact caattagcac
     6121 tcgaacgacg aagtaaagaa cgcgaaaaag cggaaaaagc agcagagaag aaacgacgac
     6181 gagaggagca gaaacagaaa gataaactga agattcgaaa actcgcctta aagccccgca
     6241 gttactggat taaacaagcc caacaagccg taaacgcctt catcagagaa agagaccgcg
     6301 acttaccatg tatctcgtgc ggaacgctca cgtctgctca gtgggatgcc ggacattacc
     6361 ggacaactgc tgcggcacct caactccgat ttgatgaacg caatattcac aagcaatgcg
     6421 tggtgtgcaa ccagcacaaa agcggaaatc tcgttccgta tcgcgtcgaa ctgattagcc
     6481 gcatcgggca ggaagcagta gatgaaatcg aatcaaacca taaccgccat cgctggactg
     6541 tcgaagagtg cagggccatc aaggcagagt accaacagaa actcaaagac ctgcgaaaca
     6601 gcagaagtga ggccgcatga cgctctcagt aaaaaccatt ccagataaca agggagaagt
     6661 cgcatgggca taagagaact aaacctcacc aaagaacagc acgattggct gaatggctgg
     6721 cttgaactgt ggggcgcatg ggtttattca ggtcgtctgg aaaagcgcat gagcagcgta
     6781 atagcgaagt tcatggagag cgtagagccg ggaagagtta tgacaagacc aatgtgtaat
     6841 gatgatgatg gaatgttgat ttctcaggtc gtcgattccg tcatgtacat tgacaagaaa
     6901 gcctttggca tcctcctcag ctactacgct catggttcat ctaagagagc aattgcatcc
     6961 tactatcacg cgactgcaaa gccacgcaag atgtgtggac gcggtggcga gggatggaga
     7021 aaaccttcac tggcaacctg tagaaacgaa attgacgata tcctgaaagc gtcattattt
     7081 gttttgtacc aaccaatgca aaatgctttc aaaatgcgta aacgtgttga gaaagttaag
     7141 catgttgctg ttaaaagcct tgacatgcaa ttagccattt agccataatt agagggtaag
     7201 ctgccgttag tgactcttaa gttgcaacgg tggctttttt tatttgggtc agtcgtataa
     7261 aggtcattac ggaaggctgt taaccttctt atcgtggttc gattcccgct acccgctcca
     7321 gatttattat caggctcgct tcggcgggct ttttttgtat ctgcgccacg cccggcgcat
     7381 atcaaccaca gagcctttcg ggggtgagct tacggagtgg tcagtgtgac tttctctgtg
     7441 ggcagatcgc tcccgggcgt tggctcaccc acccaaagga acgtcacgat gtttggtatt
     7501 tttggtaaaa aagcccgccg agcggcagtg gaaattaaaa agtttgagaa acgtgatctg
     7561 gcacaggcgg ttattaatgc tgcctatctg gtggcctatg cagatggtga atgtgaggct
     7621 tcagagaaag cgaagatcga gcaggtcttg cgtaaccagc ctgcgttgtc cgcgtttacg
     7681 tcagaaatta atgcgattag cgcaaccatt atcggtcagc tggatacgaa ctttaaaatt
     7741 ggtcgtcgag cggcgttacg tgagatcgag gatgtgaaac acgatacgcg tgaagcggaa
     7801 gacgtgctgg atgtggcggt ggccattgcc gaagcagacg gcgaaataga gccggaagag
     7861 cgcaaggtgc tggaagagat tgccggtgtc ctgggccttc gtctggagaa tcatctgtga
     7921 cggtaaaaat gcgtctggct gtggttgcgc tcctgctgtt tctggtggtg atggtggact
     7981 tcagcagccg gataatgtca gtgctggctg acggtgtgct ggtggcgggt gtggtggttg
     8041 ttgctttccc gttgctgaaa aagaaagcat caggcgatta gcagggtatc agttacccgt
     8101 tgaaattttt aaatgcctca caattcaggc ggttgactgt tgtctggttt gcggggagtt
     8161 tgttaaaaga aactggcatg gtgaatcccc ctgtgcggag gggcaatcag caactggtgt
     8221 tttgtcaccg acccttatcc tttctgtgcg ggttcaggtg ctgatactga actcaccggg
     8281 aggcacccgg caccatgcaa tggcacatag cgccactctc cagcccctct ccggaggggc
     8341 tgtttatatt gattttgtca gatgtgagta aactccttat ggactttgtt gttttagtcc
     8401 ataaggacat atttgcagag tgcaacggtt attaaagcat tcattcaata cgttatctgt
     8461 atttgtaggg cattcctggc tgtttttgat taaattccag aattttttat tgaatggtac
     8521 tacgttgtaa atggttacag gtagcacttt gttattgagc atgatacctg tgtgagtcag
     8581 tgtaaatata ctttcaggag gtaagaaagc atccgattga taccagatta ttaattttat
     8641 tttactccat atgactgaaa aagatattcc gcatgatggc tggataactg tatcaatcac
     8701 aatccacttc atttagtttc cttgtttatg tcttgctgg
//
LOCUS       DAZFQD010000184         8540 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.184, whole genome shotgun sequence.
ACCESSION   DAZFQD010000184 DAZFQD010000000
VERSION     DAZFQD010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8540
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.184"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            56..2572
                     /locus_tag="ACHKTW_004667"
     CDS             56..2572
                     /locus_tag="ACHKTW_004667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6465557.1"
                     /translation="MLQIVGALILLIAGFAILRLLFRALTSTASALAGFVLLCLFGPA
                     LLAGYITERITRLFHIRWLAGVFLTIAGMIISFMWGLDGKHIALEAHTFDSVKFILTT
                     ALAAGLLALPVQIRSIQQNGLTPEDISKEINGYYCCFYTAFFLMACSAYAPLIALQFD
                     ISPSLMWWGGLLYWLAALVTLLWAASQIQALKRLTSAIRQTLEEQPVLNSKSWLSSLQ
                     NDYSLPETLTERIWLTLISQRISRGELREFELADGNWLLDNAWYERNMAGFNEKLRES
                     LSFTPDELKTLFRNRLNLSPEANDDFLDRCLDGGDWYPFSEGRRFVSFHHVDELRICA
                     SCGLTEVHHAPENHKPDPEWYCSSLCRETETLCQDIYERSYTGFISDATANGLILMKL
                     PETWSTNEKMFASGGQGHGFAAERGNHIVDRVRLKNARILGDNNARNGADRLVSGTEI
                     QTKYCSTAARSVGAAFDGQNGQYRYMGNHGPMQLEVPRDQYAGAVETMKNKIREGKVP
                     GVTDPAEASRLIRRGHLTYTQARNITRFGTIESVTYDIAEGSVVSLAAGGISFALTAS
                     VFWLSTGDRDAALQTAAVQAGKTFTRTLAVYVTTQQLHRLTVVQGMLKHIDFSTASPT
                     VRQALQKGTGAGNISALNKVMKGTLVTSLALVAVTTGPDMIKMLRGRISGAQFIRNLA
                     VASSGVAGGAVGSVAGGILFSPLGPFGALTGRVVGGVLGGMIASAVSGKIAGALVEED
                     RVKILAMIQEQVTWLAGSFLLTGHEIENLNANLARVIDQNALEIIFAAGIQQRAATNM
                     LIKPLVVSIIRQRPVMEYDASHLGNMVNRLEEALPPELPA"
     gene            2648..3103
                     /locus_tag="ACHKTW_004668"
     CDS             2648..3103
                     /locus_tag="ACHKTW_004668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IrmA family protein"
                     /protein_id="HFO6465558.1"
                     /translation="MIHLFKTGMITAFILGLTWSAPLRAQDQRYISIRNTDTIWLPGN
                     ICAYQFRLDNGGNDEGFGPLTITLQLKDKYGQTLVTRKMETEAFGDSNATRTTDAFLE
                     TECVENVATTEIIKATEESNGHRVSLPLSVFDPQDYHPLLITVSGKNVN"
     gene            3138..3266
                     /locus_tag="ACHKTW_004669"
                     /pseudo
     CDS             3138..3266
                     /locus_tag="ACHKTW_004669"
                     /inference="COORDINATES: protein motif:HMM:NF017787.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3222..3410)
                     /locus_tag="ACHKTW_004670"
     CDS             complement(3222..3410)
                     /locus_tag="ACHKTW_004670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6465559.1"
                     /translation="MSEKPSTGEPRIKLFSGISFRVPAASASLTFRVADNPTEMHSRV
                     ILDKHIAVSGYDSNRLPV"
     gene            3514..4335
                     /locus_tag="ACHKTW_004671"
     CDS             3514..4335
                     /locus_tag="ACHKTW_004671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFO6465560.1"
                     /translation="MRLVSRFGYAANQIRRDRPLTHEELMHHVPGIFGEEKHTSRSQN
                     YTYIPTITVLESLQREGFQPFFACQTRVRDPGRRGYTKHMLRLRRAGEINGEHVPEII
                     LLNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVDRVIEGAYEVVGVF
                     DRIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLW
                     SAYQTIQENMLKGGISGRSAKGKRIHTRAIHSIDTDIKLNRALWVMAETLLESMR"
     gene            4335..4580
                     /locus_tag="ACHKTW_004672"
     CDS             4335..4580
                     /locus_tag="ACHKTW_004672"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6465561.1"
                     /translation="MKHIPEGVIQAPESPDAPEALPGCRSISDDDLCAWCTRLLYRPG
                     ELSLCRLSTGNGIWPSVCDRNGYAYGCPEFQPNIIHP"
     gene            4674..5147
                     /locus_tag="ACHKTW_004673"
     CDS             4674..5147
                     /locus_tag="ACHKTW_004673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFO6465562.1"
                     /translation="MKTLSQNTTSSACAPETGLQQLVATIVPDEQRISFWPQHFSLIP
                     QWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNAMNGN
                     RAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRIID"
     gene            5163..5639
                     /gene="yeeS"
                     /locus_tag="ACHKTW_004674"
     CDS             5163..5639
                     /gene="yeeS"
                     /locus_tag="ACHKTW_004674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HFO6465563.1"
                     /translation="MQQLSFLPGAMTPGERSLIQRALKTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNKLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGSQVFSFAEHGLL"
     gene            5708..5929
                     /gene="yeeT"
                     /locus_tag="ACHKTW_004675"
     CDS             5708..5929
                     /gene="yeeT"
                     /locus_tag="ACHKTW_004675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HFO6465564.1"
                     /translation="MKIITRGEAMRIHQQHPASRLFPFCTGKYRWHGSTEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            6008..6376
                     /gene="cbeA"
                     /locus_tag="ACHKTW_004676"
     CDS             6008..6376
                     /gene="cbeA"
                     /locus_tag="ACHKTW_004676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system cytoskeleton
                     bundling-enhancing antitoxin CbeA"
                     /protein_id="HFO6465565.1"
                     /translation="MSDTLPGTTHPDDNHDRPWWGLPCTVTPCFGARLVQEGNQLHYL
                     ADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSGELNPRHQHTVTLYAKGLTCEAD
                     TLGSCGYVYLAVYPTPEMKN"
     gene            6465..6842
                     /gene="cbtA"
                     /locus_tag="ACHKTW_004677"
     CDS             6465..6842
                     /gene="cbtA"
                     /locus_tag="ACHKTW_004677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000854828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system
                     cytoskeleton-binding toxin CbtA"
                     /protein_id="HFO6465566.1"
                     /translation="MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSAGAPSQLINSIDILRARRATG
                     LMTRNNYRMVNNITQGKYPEAKR"
     gene            6839..7327
                     /locus_tag="ACHKTW_004678"
     CDS             6839..7327
                     /locus_tag="ACHKTW_004678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HFO6465567.1"
                     /translation="MKLALTLEADSVNVQALNMGRIVVDADGVELAELINVVCDNGYS
                     LRVVDESDRTSTDSIPPSAALSGIRCSTAHITAKDNAWLYSLSHQTNDTGESEWIHFT
                     GSGYLLRTDAWSYPVLRLKRLGLSRTFRRLVVTLTRRYGVSLIHLDASAECLPDLPTF
                     DW"
     gene            7339..7536
                     /locus_tag="ACHKTW_004679"
     CDS             7339..7536
                     /locus_tag="ACHKTW_004679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HFO6465568.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCESGIIFDNDEDKTDSAALLPC
                     IEQAREDIRTLRQLQLPDQNR"
     gene            7621..8466
                     /locus_tag="ACHKTW_004680"
     CDS             7621..8466
                     /locus_tag="ACHKTW_004680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFO6465569.1"
                     /translation="MSAITVSRPEVVNGHTDVICSTSIRHILAVRKSTLLQIDTLIRQ
                     LAEISAMTESIGGKTARDWAMKQDFRCGCWLMEKTETAMKAITRNLDREIWRDLMQRS
                     GMLSLMDAQARDTWYRSLEYDNFPEISEANILSTFEQLHQNKDEVFERGVINVFRGLS
                     WNYKTNSPCKFGSKIIVNNLVRWDRWGLHLITGQQADRLADLERMLHLFSGKPIPDSR
                     ENIIIRLDDHIRSVQGKASYEDEMFNIRYFKKGSAHITFRKPELVDRLNDIIARHYPE
                     MLPSL"
BASE COUNT         2064 a         2269 c         2237 g         1970 t
ORIGIN      
        1 tcagtgccac attcactggc attcacaata acatgatatt catcacggag tgactatgtt
       61 acagatagtc ggcgcgctga tcctgctgat cgcaggattt gccattcttc gccttttgtt
      121 cagagcatta accagcacgg cgtctgcgct ggcaggattc gtattgctgt gtctgttcgg
      181 cccggcttta ctggctggct atatcactga acgcataacc cggttattcc atattcgctg
      241 gctggcaggc gtatttctga cgattgccgg aatgatcatc agcttcatgt ggggacttga
      301 tggtaaacat atcgcactgg aggctcatac ttttgactct gtaaaattta ttctgaccac
      361 cgctctcgcc gctggtctgc tggctcttcc cgtgcagata agaagcattc agcagaacgg
      421 gctcacacct gaagatatca gcaaggaaat taacgggtat tactgctgtt tttatactgc
      481 ttttttcctt atggcgtgtt ctgcatacgc accattgatc gcattgcagt tcgatatttc
      541 accctcactg atgtggtggg gcgggttgtt gtactggctg gctgcattag tgacgctgct
      601 atgggcggcc agccagatcc aggcgctgaa aagactgacc agtgccatcc gccagacact
      661 ggaagaacaa ccggtgctca acagtaaatc gtggctgagc agtttgcaaa acgattacag
      721 ccttcctgaa acgctgacgg agcgcatctg gctgacactc atttcacaac ggatttcccg
      781 gggagaactg agggaatttg aactggcaga cggaaactgg ctactggaca atgcctggta
      841 tgaaagaaac atggcgggtt tcaacgaaaa gctgagagag agcctgtcat ttacccctga
      901 tgaactgaaa accctcttcc ggaaccgcct gaatttatca ccggaagcga atgacgattt
      961 tctcgatcgt tgcctggacg gcggtgactg gtaccccttt tcagaaggcc gccgttttgt
     1021 atcattccac cacgtggatg agcttcgtat ctgtgcctcc tgcgggctga cagaagtaca
     1081 tcatgccccg gaaaatcata agccggatcc ggaatggtac tgctcctctc tttgtcgcga
     1141 aacagaaaca ctgtgtcagg acatttatga acgttcttac accggtttta tttccgatgc
     1201 aacggcgaat ggtctgattc tcatgaaact gccggaaacc tggagtacaa atgagaaaat
     1261 gtttgcttcc ggagggcagg gacatgggtt tgccgctgaa cggggaaacc atattgtcga
     1321 cagagtccgt ctgaaaaacg cacggatcct cggtgataat aatgccagaa atggagcaga
     1381 cagactggtc agcggaacag aaatccagac gaaatattgt tcaactgcag cccgtagcgt
     1441 cggtgcggca ttcgacggac aaaacggaca gtatcgttac atgggaaatc atggtcccat
     1501 gcaactggaa gtcccccgtg atcagtatgc cggcgctgtg gaaaccatga agaataagat
     1561 ccgcgaaggt aaagtacccg gtgtaaccga tcccgcagaa gcgtcccggc tgattcgtcg
     1621 gggacatctg acttataccc aggcccgtaa tatcacccgg ttcgggacca tcgaatcggt
     1681 cacttatgat attgccgagg ggtcggttgt cagtctggcg gccggaggga tcagttttgc
     1741 cctgacggca tcggtcttct ggctcagcac cggcgatcgc gatgctgccc tgcagacagc
     1801 tgctgtccag gcaggaaaaa ccttcacccg cacactggct gtctacgtca caacccagca
     1861 acttcaccgg ctcactgttg ttcagggtat gctgaagcat attgattttt cgacggccag
     1921 cccgactgtc cggcaggcgc ttcagaaggg gaccggtgca ggaaatatca gtgccctgaa
     1981 caaagtgatg aagggtacgc tggtgacatc tctggcactg gtcgctgtca caaccggccc
     2041 tgacatgatc aaaatgttgc ggggacggat ctccggtgcg cagttcatca ggaatcttgc
     2101 cgtggcatct tccggtgtgg caggtggtgc tgtcgggtca gtggcgggcg ggatattgtt
     2161 cagtccactg ggaccatttg gtgcactgac aggacgtgtg gttggcggtg ttctgggggg
     2221 aatgattgcc tccgctgtat caggaaaaat tgccggagcg ctggttgaag aagatcgcgt
     2281 caaaattctg gcaatgattc aggagcaggt gacatggctt gccggcagtt tcctgctgac
     2341 cggacatgag attgaaaatc tgaacgcgaa tctggcccgt gttatcgatc agaatgctct
     2401 ggagatcatt ttcgccgccg gtatacaaca acgggccgcg accaatatgt taatcaaacc
     2461 actggtggtc agtatcatca ggcaacgccc cgtcatggaa tatgatgcat cccatctcgg
     2521 caatatggtt aaccgactgg aagaagcatt acccccggag ttaccggcat aacggctatt
     2581 ctgcgccttt tacgttttat cacctgattt gtcagtgtca tcgtcatgac tcagagaggt
     2641 aaataccatg attcacctgt tcaaaaccgg catgattacc gccttcattc tggggttaac
     2701 gtggtctgcc ccactccggg cacaggatca acgttacatc agtatacgca atacagatac
     2761 gatatggctc ccgggaaata tttgtgccta ccagttccgg ctggataatg gcggaaacga
     2821 tgaaggattt ggccccctca ccatcactct gcaactcaaa gacaaatatg gtcagacgct
     2881 ggtgaccaga aaaatggaaa cggaagcctt tggtgacagt aatgccacgc gaaccacaga
     2941 cgcatttctg gaaacggagt gcgtggaaaa tgtcgccaca accgaaatca ttaaagcaac
     3001 tgaagaaagt aacggccatc gtgtcagtct gccgttatcg gttttcgatc cccaggacta
     3061 ccatccactg ctgattaccg tttccggaaa aaatgttaac tgatccctga agtcagtttc
     3121 atgcttcagc ctctgccata cgttgtggca gaggctattt atcaggagaa accagatatg
     3181 ccgggttaca cacagagtat gtcctggctg aaggatcttt ctcatacggg caggcggttg
     3241 ctgtcataac cgcttaccgc aatgtgttta tccaggatga cccgggaatg catttccgtc
     3301 gggttatccg caacgcggaa ggtcagcgac gctgacgctg ccggaactct gaagctgatg
     3361 ccggaaaaca gcttaatgcg tggctcgcca gtggagggct tctcagacaa taaacctcat
     3421 taatttcacc catcaggccg cgtcttctcc gggagacgcg gcttttttca tttattacac
     3481 cagtaaatct taaccaccga taaggagcaa agtatgcgat tagtcagtcg ttttggttat
     3541 gctgcaaacc agatacgccg tgaccgtccg ctgacacatg aagagctgat gcaccatgtg
     3601 ccggggattt tcggggaaga gaaacacacg tcccgaagcc agaactatac gtacatcccc
     3661 accatcaccg tactggaaag tctgcagcgg gaaggcttcc agcccttttt cgcctgccag
     3721 acccgtgtgc gcgacccggg ccgccgggga tacacaaaac acatgctgcg tctgcggcgg
     3781 gccggagaga taaacggaga acatgtccct gaaattattc tgctcaactc ccatgacggt
     3841 acctccagct accagatgct gccgggttac ttcaggttcg tctgccagaa cggatgcgtc
     3901 tgtggtcagt ctctggggga agtgcgtgtt ccgcaccggg gaaatgtggt ggacagagtc
     3961 attgaagggg cttacgaggt ggtgggcgtg tttgaccgga tagaggagaa gcgtgatgcc
     4021 atgcagtcgc tggtcctgcc gccaccggca cgccaggcgc tggcacaggc ggcactgact
     4081 taccgttatg gtgacgaaca tcagcccgtc actaccgccg acattctgac accgcgacgc
     4141 cgggaggatt acggtaagga cctgtggagt gcttatcaga ccatccagga gaatatgctg
     4201 aaaggcggga tttccggtcg cagtgcaaaa ggaaaacgta tccacacccg tgccattcac
     4261 agcatcgaca ccgatattaa gctcaaccgc gcattgtggg tgatggcaga aacgctgctg
     4321 gagagcatgc gctgatgaaa catatccctg aaggcgtgat tcaggcacca gaatcacccg
     4381 acgccccgga agcgctgccg gggtgtcgtt ccatttctga tgatgacctg tgcgcctggt
     4441 gtacccgtct gctttaccgt cccggagaac tcagtctgtg cagactgagt acaggcaatg
     4501 gcatctggcc atccgtctgt gaccggaacg gttacgcgta cggctgtccg gaatttcagc
     4561 cgaatatcat tcatccctga tattgccggg tctcacggtc agtgatgaac gtgatgttgt
     4621 cagcccccgg cctttatttc ctctcattta ttcacattac aaggatttca cacatgaaaa
     4681 cattgtctca gaacaccacc tcttcagcct gtgcaccgga gactggcctg caacaactgg
     4741 ttgccaccat agtccctgat gaacagcgca tcagcttctg gccgcagcat tttagcctca
     4801 ttccacagtg ggtgaccctg gagccccgtg tcttcggctg gatggaccgt ctgtgcgaag
     4861 actactgcgg gggcatctgg aatctgtaca ccctgaacaa cggcggggca tttatggccc
     4921 ctgaaccgga tgacgatgat gacgaaacat gggtgctgtt taacgccatg aacggcaacc
     4981 gcgctgaaat gagcccggaa gccgccggca ttgctgcctg tctgatgacg tacagtcatc
     5041 atgcctgtcg cacggagtgt tatgccatga cggttcatta ttaccgtctg cgggactacg
     5101 ccctgcagca tccggaatgc agcgccatta tgcgcatcat tgactgaaag ggggccggaa
     5161 caatgcaaca actttccttt ctgcccggcg cgatgacgcc cggcgagcgc agcctcattc
     5221 agcgggccct gaaaaccctg gaccgccatc ttcatgaacc cggcgtggcc ttcacctcca
     5281 cccgtgcggc ccgggaatgg ctgattctga acatggctgg actggagcgt gaagaattcc
     5341 gggtgctgta tctgaacaac cagaataagc tgattgccgg tgaaaccctc ttcaccggca
     5401 ccatcaaccg tacggaagtt catccccggg aagtgattaa acgcgccctg taccacaatg
     5461 ctgctgccgt ggtgctggcg cacaatcacc cgtccggtga agtcacaccc agcaaggcag
     5521 accgccttat cacggaacgt ctggtacagg cactgggcct ggtggatatc cgggtgccgg
     5581 accatctgat agtcggtggc agccaggttt tctcctttgc cgaacacggt ctgctttaac
     5641 ccgtcaccgt cacaaccaca tcacacctgt tttcgctttt atcttctgtc ttcagaggta
     5701 tcacactatg aaaatcatca cccgtggtga agccatgcgt attcaccaac aacatccggc
     5761 atcccgtctt tttccgttct gtaccggcaa ataccgctgg cacggcagca ctgaagcgta
     5821 taccggtcgt gaagtgcagg atattcccgg tgtgctggct gtatttgcag aacgccgtaa
     5881 agacagtttt ggcccgtatg tccggctgat gagcgtcacc ctgaactgag ccaggaaggg
     5941 cattctgatg agccgaatta tcgccaccat cgaaccattc ttaactgatt tctgtgagga
     6001 ctttatcgtg tcagacacac tgcccgggac aacgcatccc gacgacaatc acgaccgccc
     6061 ctggtggggg ctgccctgca ccgtgacgcc ctgtttcggg gcacgtctgg tgcaggaggg
     6121 taaccagttg cattaccttg ccgaccgtgc cggtatcaga ggccggttca gcgacgcgga
     6181 tgcataccac ctggaccagg cctttccgct gctgatgaaa caactggaac tcatgctcac
     6241 cagcggtgaa ctgaatcccc gccatcagca taccgtcaca ctgtatgcga aagggctgac
     6301 ctgcgaagcc gacaccctcg gctcctgtgg ttacgtttat ctggctgttt atccgacgcc
     6361 cgaaatgaaa aattaactct ccagaatagc cttctgctac ggcctggtgt tttcaccacg
     6421 ccacttttcc atttttatat ctgcatatca ggaaaatctt cagtatgaaa acattacctg
     6481 tattacccgg gcaggcggcc agttctcgcc cgtctcctgt tgaaatctgg cagatactgc
     6541 tgtcccgact gctggaccag cactatggcc tcacgctgaa tgacacaccg ttcgctgatg
     6601 aacgtgtgat tgagcagcat attgaggcag ggatttcact gtgtgacgcg gtgaactttc
     6661 tcgttgaaaa atacgcgctg gtacgtaccg accagccggg attcagcgca ggagccccgt
     6721 cgcagttaat caacagcatt gatattctcc gggctcgcag ggcgaccggc ctgatgaccc
     6781 ggaacaatta cagaatggta aataacatta cccagggcaa gtatccggag gcaaaacgat
     6841 gaaactggcc ctgacgctgg aagctgacag cgttaacgta caggcactga acatggggcg
     6901 aattgtcgtt gacgccgatg gtgttgagct tgccgaactg attaacgtgg tctgcgataa
     6961 tggctactcc cttcgtgttg ttgatgaatc tgaccggacc tcaacagaca gcataccccc
     7021 ctctgctgcc ctgagcggta tacgctgcag taccgcgcat atcacggcaa aagacaacgc
     7081 ctggctgtac tcgctgtcgc atcagacgaa tgacaccggc gaatcagaat ggattcattt
     7141 tacaggcagc ggatatctgt tacgtaccga tgcgtggtca tacccggttc tgcggcttaa
     7201 acgcctgggc ctgtccagaa cgttccgccg tctggttgtc acacttaccc gacgttatgg
     7261 cgtcagtctc attcatctgg atgccagcgc cgaatgtctg ccggatttac ccactttcga
     7321 ctggtaacca gaaacaacat gaaatcatta accacggaaa cagcgctgga tattctgatt
     7381 gcatggctgc aggacaatat cgactgcgaa tcgggaatta tcttcgacaa cgatgaagat
     7441 aaaacggatt cggcagcact gttgccctgt attgaacagg ccagggagga tatccgtacc
     7501 ctgcgccagc tgcagcttcc agaccagaac cggtgagtct cattcatcat acttcattcc
     7561 actgacgcca gcctgaacag ggctggcgtt ttcatttatc tgcgaaaagg aatatcgatt
     7621 atgtctgcaa tcacagtctc ccgtccggaa gtggtcaacg ggcatacgga cgttatctgc
     7681 tcaaccagta tccgccacat tctggctgta cgaaaaagta cactgctgca aatcgacaca
     7741 cttatccggc aactggctga aatctcagca atgacggaaa gcattggcgg caaaaccgcc
     7801 cgggactggg ccatgaaaca ggattttcgc tgcggctgct ggctgatgga gaaaacggaa
     7861 accgcaatga aagctatcac ccgcaacctt gaccgcgaaa tctggcgtga cctgatgcaa
     7921 cgttcaggga tgctgtcatt aatggatgca caggcccgtg atacatggta ccggtcactg
     7981 gagtacgata attttccgga aatcagcgag gcgaatatcc tgagcacatt tgaacaactg
     8041 caccagaaca aagatgaagt gtttgagcga ggggtgatca acgtcttcag agggctgagc
     8101 tggaattaca aaaccaactc cccctgtaaa tttggcagta aaattatcgt caacaatctg
     8161 gtgaggtggg accgatgggg attgcatctt atcaccgggc aacaggcaga tcgactcgcc
     8221 gacctggaaa gaatgctgca tctgttcagc ggcaaaccga tccccgacag ccgggaaaat
     8281 atcatcattc ggctggatga tcacatccgc tccgttcagg gcaaagcgag ctatgaagat
     8341 gagatgttca acatcagata ctttaagaag ggctccgcac acatcacgtt caggaaacca
     8401 gaactggttg atcggctaaa tgatattatt gccaggcact atccggaaat gttaccttca
     8461 ttataaagct ctaagaatga atgcaattat gtattttagc ttcattccct cacgcaaatt
     8521 tatttggggg ttcttatggg
//
LOCUS       DAZFQD010000185         8412 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.185, whole genome shotgun sequence.
ACCESSION   DAZFQD010000185 DAZFQD010000000
VERSION     DAZFQD010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8412
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.185"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            98..2476
                     /gene="ppsA"
                     /locus_tag="ACHKTW_004681"
     CDS             98..2476
                     /gene="ppsA"
                     /locus_tag="ACHKTW_004681"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HFO6465570.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPNEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            complement(2533..4179)
                     /gene="fadK"
                     /locus_tag="ACHKTW_004682"
     CDS             complement(2533..4179)
                     /gene="fadK"
                     /locus_tag="ACHKTW_004682"
                     /EC_number="6.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
                     /protein_id="HFO6465571.1"
                     /translation="MKVTLTFNEQRRAAYRQQGLWGDASLADYWQQTARAMPDKIAVV
                     DNHGASYNYSALDHAASCLANWMLAKGIESGDRIAFQLPGWCEFTVIYLACLKIGAVS
                     VPLLPSWREAELVWVLNKCQAKMFFAPTLFKQTRPVDLILPLQNQLPQLQQIVGVDKL
                     APATSSLSLSQIIADNTPLTTAITTHGDELAAVLFTSGTEGLPKGVMLTHNNILASER
                     AYCARLNLTWQDVFMMPAPLGHATGFLHGVTAPFLIGARSVLLDIFTPDACLALLEQQ
                     RCTCMLGATPFVYDLLNVLEKQPADLSALRFFLCGGTTIPKKVARECQQLGIKLLSVY
                     GSTESSPHAVVNLDDPLSRFMHTDGYAAAGVEIKVVDDARKTLPPGCEGEEASRGPNV
                     FMGYFDEPELTARALDEEGWYYSGDLCRMDEAGYIKITGRKKDIIVRGGENISSREVE
                     DILLQHPKIHDACVVAMPDERLGERSCAYVVLKAPHHSLSLEEVVAFFSRKRVAKYKY
                     PEHIVVIEKLPRTASGKIQKFLLRKDIMRRLTQDVCEEIE"
     gene            complement(4236..4529)
                     /locus_tag="ACHKTW_004683"
     CDS             complement(4236..4529)
                     /locus_tag="ACHKTW_004683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HFO6465572.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILAANPDINEFRKLMKA
                     CPAGLYKQDDAGNIHFDSAGCLECGTCRVLCGNTILEQWQYPAGTFGIDFRYG"
     gene            complement(4526..5815)
                     /locus_tag="ACHKTW_004684"
     CDS             complement(4526..5815)
                     /locus_tag="ACHKTW_004684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFO6465573.1"
                     /translation="MSEDKFDAIVVGAGVAGSVAALVMARAGLDVLVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAVSAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILEANVVILAD
                     GVNSMLGRSLGMVPASDPHHYAVGVKEVIGLTPEQINDRFNITGEEGAAWLFAGSPSD
                     GLMGGGFLYTNKDSISLGLVCGLGDIAHAQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SGHMVPEGGLAMVPQMVNDGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KEREDFSASSLAQYKRELEQSCVMRDMQHFRKIPALMENPRLFSQYPRMVADIMNEMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            complement(5871..6809)
                     /locus_tag="ACHKTW_004685"
     CDS             complement(5871..6809)
                     /locus_tag="ACHKTW_004685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HFO6465574.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGGAQQWGQQVYAIVQNTDQAQAV
                     MPYGPKCIYVLEQNDALQRTENYAESIAALLKDKHPAMLLLAATKRGKALAARLSVQL
                     NAALVNDATAVDIVDGHICAEHRMYGGLAFAQEKINSPLAIITLAPGVQEPCTSDTSH
                     QCPTETVPYVAPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAA
                     VLNAEVGCSRPIAEGENWMERERYIGVSGVLLKSDLYLTLGISGQIQHMVGGNGAKVI
                     VAINKDKNAPIFNYADYGLVGDIYKVVPALISQLSR"
     gene            complement(6829..7593)
                     /locus_tag="ACHKTW_004686"
     CDS             complement(6829..7593)
                     /locus_tag="ACHKTW_004686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HFO6465575.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQLATDDDEIAALTVGGSLLQNSKVRKDVLSRGPHSLYLVQDAQLEHALPLDTAKALA
                     AAVEKIGFDLLIFGEGSGDLYAQQAGLLVGEILQLPVINAVSAIQRQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTSDINVPRIPSMKAILGAGKKPVNQWQASDIDWSQSAPLAEL
                     VGIRVPPQTERKHIIIDNDSPEAIAELAEHLKKALN"
     gene            7909..>8412
                     /locus_tag="ACHKTW_004687"
     CDS             7909..>8412
                     /locus_tag="ACHKTW_004687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HFO6465576.1"
                     /translation="MYQRCFDNASETLFVAGKTPRLSRFAISDDPKWESGHHVHDNET
                     ELIYVKKGVARFTIDSSLYVAHADDIVVIERGRLHAVASDVNDPATTCTCALYGFQFQ
                     GAEENQLLQPHSCPVIAAGQGKEVIKTLFNELSVILPQSKNSQTSSLWDAFAYTLAIL
                     YYENFKNA"
BASE COUNT         2007 a         2178 c         2180 g         2047 t
ORIGIN      
        1 aacgattcac cgtttttttc atccggttaa atatgcaaag ataaatgcgc agaaatgtgt
       61 ttctcaaacc gttcatttat cacaaaagga ttgttcgatg tccaacaatg gctcgtcacc
      121 gctggtgctt tggtataacc aactcggcat gaatgatgta gacagggttg ggggcaaaaa
      181 tgcctccctg ggtgaaatga ttactaacct ttccggaatg ggtgtttccg ttccgaatgg
      241 tttcgccaca accgccgacg cgtttaacca gtttctggac caaagcggcg taaaccagcg
      301 catttatgaa ctgctggata aaacggatat tgacgatgtt actcagcttg cgaaagcggg
      361 cgcgcaaatc cgccagtgga ttatcgacac tcccttccag cctgagctgg aaaacgccat
      421 ccgcgaagcc tatgcacagc tttccgccga tgacgaaaac gcctcgtttg cggtgcgttc
      481 ctccgctact gcagaagata tgccggacgc ttcttttgcc ggtcagcagg aaaccttcct
      541 caacgttcag ggttttgacg ccgttctcgt ggcagtgaaa catgtatttg cttctctgtt
      601 taacgatcgc gccatctctt atcgtgtgca ccagggttac gatcaccgtg gcgtggcgct
      661 ctccgccggt gttcagcgga tggtgcgctc cgacctcgca tcttctggcg tgatgttctc
      721 cattgatacc gaatccggct ttgaccaggt ggtgtttatc acttccgcat ggggccttgg
      781 ggaaatggtc gtgcagggtg cggttaaccc ggatgagttt tacgtccata aaccgacact
      841 ggcggcgaat cgcccggcta tcgtgcgccg caccatgggg tcgaaaaaaa tccgcatggt
      901 ttatgcgccg acccaggagc acggcaagca ggttaaaatc gaagacgtac cgcaggaaca
      961 gcgtgacatc ttctcgctga ccaacgaaga agtacaggaa ctggcaaaac aggccgtaca
     1021 aattgagaaa cactacggtc gcccaatgga tattgagtgg gcgaaagatg gccacaccgg
     1081 caaactgttc attgtgcagg cgcgtccgga aaccgtgcgc tcacgcggtc aggtcatgga
     1141 gcgttatacg ctgcattcac agggtaagat tatcgccgaa ggccgtgcta tcggtcatcg
     1201 catcggtgcg ggtccggtga aagtcatcca tgacatcagc gaaatgaacc gcatcgaacc
     1261 tggtgacgtg ctggtcactg atatgaccga cccggactgg gaaccgatca tgaagaaagc
     1321 atctgccatc gtcaccaacc gtggcggtcg tacctgtcac gcggcgatca tcgctcgtga
     1381 actgggcatt ccggcggtag tgggctgtgg agatgcaaca gaacggatga aagacggtga
     1441 gaacgtcact gtttcttgtg ccgaaggtga taccggttac gtctatgcgg agttgctgga
     1501 atttagcgtg aaaagctcca gcgtagaaac gatgccggat ctgccgttga aagtgatgat
     1561 gaacgtcggt aacccggacc gtgctttcga cttcgcctgt ctgccgaacg aaggcgtggg
     1621 ccttgcgcgt ctggaattta tcatcaaccg tatgattggc gtccacccac gcgcactgct
     1681 tgagtttgac gatcaggaac cgcagttgca aaacgaaatc cgcgagatga tgaaaggttt
     1741 tgattctccg cgtgaatttt acgttggtcg tctgactgaa gggatcgcga cgctgggtgc
     1801 cgcgttttat ccgaagcgcg tcattgtccg tctctctgat tttaaatcga acgaatatgc
     1861 caacctggtc ggtggtgagc gttacgagcc agatgaagag aacccgatgc tcggcttccg
     1921 tggcgcgggc cgctatgttt ccgacagctt ccgcgactgt ttcgcgctgg agtgtgaagc
     1981 agtgaaacgt gtgcgcaatg acatggggct gaccaacgtt gagatcatga tcccgttcgt
     2041 gcgtaccgta gatcaggcga aagcggtggt tgaagaactg gcgcgtcagg ggctgaaacg
     2101 tggcgagaac gggctgaaaa tcatcatgat gtgtgaaatc ccgtccaacg ccttgctggc
     2161 agagcagttc ctcgaatatt tcgacggctt ctcaattggc tcaaacgaca tgacacagct
     2221 ggcgctcggt ctggaccgtg actccggagt ggtgtctgaa ctgttcgatg agcgcaacga
     2281 tgcggtgaaa gcactgctgt cgatggcgat tcgtgccgcg aagaaacagg gcaaatatgt
     2341 cgggatttgc ggtcagggtc cgtccgacca tgaagacttt gccgcatggt tgatggaaga
     2401 ggggatcgat agcctgtctc tgaacccgga caccgtggtg caaacctggt taagcctggc
     2461 tgaactgaag aaataaaata aatccccggc ggcgttcagt cgccgggttt atgtgatccc
     2521 cgaagatgaa acttattcaa tctcttcaca gacatcctgc gttaatcgcc gcataatatc
     2581 ttttcttaac aaaaactttt gtattttacc tgaggcagtg cgcggtagtt tttcgattac
     2641 cacgatatgt tcaggatatt tatattttgc gacccgttta cggctaaaaa aagccaccac
     2701 ctcttccagc gataatgaat gatgcggcgc tttcagcacg acataagcgc atgatcgttc
     2761 acctaaacgt tcatcgggca ttgcaaccac acaggcatcg tgaattttag gatgctgcaa
     2821 taaaatatct tccacttcac ggctgctaat attttcgccg ccgcggacaa taatatcttt
     2881 tttgcgtccg gtaattttta tatagccagc ctcatccata cggcagagat cgccgctgta
     2941 ataccagcct tcttcatcca gcgcacgggc ggttaattca ggttcatcaa aatatcccat
     3001 aaacacattg gggccacgcg aggcttcttc accttcgcaa cctggcggta aggtcttgcg
     3061 tgcgtcatcg accactttaa tctctacacc tgcggcagcg taaccatcgg tgtgcataaa
     3121 gcgcgacaaa ggatcatcga gattcaccac cgcatgcggc gaactttctg tggaaccata
     3181 aacacttaat aatttaatgc cgagctgctg gcattcacgc gccacttttt tggggattgt
     3241 ggttccgccg caaagaaaga aacgcagcgc tgacaggtcc gcgggttgtt tctctagtac
     3301 attcaaaaga tcatagacaa acggcgttgc gcctagcata caggtgcaac gctgctgctc
     3361 aagcagcgcg agacacgcat caggagtgaa aatatctaac aacacgctgc gagcgccaat
     3421 taagaatggt gccgttacgc catgcagaaa gcccgttgcg tggccgagtg gcgcaggcat
     3481 cataaagaca tcctgccagg tcagattcag ccgcgcgcaa taagcccgct cactggcgag
     3541 aatattgtta tgcgttagca tcacgccctt tggcagaccc tcggttccgg aggtaaacag
     3601 caccgcagct aattcatcgc cgtgggtcgt tatcgccgtg gtcagtgggg tattgtcggc
     3661 gataatctga cttaatgaga gggaagaggt ggcgggagcc agtttgtcca cgccgacaat
     3721 ttgttgtagt tgtggaagct gattttgcag cggcaggatt aaatctaccg ggcgcgtttg
     3781 tttaaacaac gtcggtgcaa agaacatttt tgcctgacac ttattgagca cccacaccag
     3841 ttctgcttcc cgccaggaag gcaacagcgg cacggaaacc gcaccgattt tcaggcaggc
     3901 aagatagata acggtaaatt cacaccagcc aggcagttga aatgcgatgc gatcgcctga
     3961 ttcaataccc ttcgctaaca tccagtttgc cagacagctc gcggcgtgat cgagcgcgct
     4021 atagttgtac gatgcaccat gattatcgac cacggcaatt ttgtctggca tcgcacgagc
     4081 ggtctgctgc cagtaatcgg ccagcgaagc atcgccccat agcccttgct gacgatacgc
     4141 cgcacgacgt tgttcgttga acgttaatgt cactttcatg ttggactctc gaaacagaac
     4201 atcaggcccg agatgcgggc ctgtgggatg catgattagc cgtagcgaaa atcaataccg
     4261 aaggtgcctg cgggatattg ccactgttcg agaatagtgt taccgcacag cacccgacag
     4321 gtgccgcact ccagacaacc ggcagaatca aaatgaatgt tgccggcgtc atcctgctta
     4381 taaagtccgg cagggcaggc tttcattaat ttacggaatt cattgatatc gggatttgcc
     4441 gccagaatga tatgcgggtg gccctcatca acatggaatt tattgacgcc taatttgatg
     4501 tcgacgttaa ccgtagcgtt ctggctcata gcgcggttgc tcccttaatg ccatctttca
     4561 gcaagttgat cagcccaatt ttcttcgcat gtcccatgat cattttgcgt accggctggt
     4621 ttggtttgcc gtcaatggtg aacatctcgt tcatgatgtc ggcgaccatt cgtgggtatt
     4681 ggctaaacag gcgcgggttt tccatcagcg ccgggatctt gcggaaatgc tgcatatcgc
     4741 gcatgacgca gctttgttcc agctcacgtt tgtattgcgc cagactgctg gcggagaaat
     4801 cctcgcgttc tttggcggcg atcaccgttg tggcggcagc ctgagccgat gcaatggcta
     4861 aatccatgcc acgaactgta aaacccaaat tcaggcagaa gcctgcggcg tcaccaacga
     4921 tcatcacgcc atcgttaacc atctgcggca ccattgccag accgccttct ggcaccatat
     4981 gcccggaata ttcaagcagt ttgccgccgc taattagtgg gcgaatggcg gggtgttgtt
     5041 taaaatcttc cagcatttgc ggcacgcttt tttgcgcatg ggcgatatca cccaatccac
     5101 aaaccagccc caaggatatg gaatccttgt tggtatagag aaatcccccg cccatcaggc
     5161 cgtcagaagg ggaaccggca aacagccagg cggcaccttc ctcgcccgta atattaaagc
     5221 gatcgttgat ctgttctggt gtgaggccaa taacctcttt aacaccaaca gcgtaatgat
     5281 gcggatcgga agcgggaacc attcccagcg agcggccaag catcgagtta acgccatcag
     5341 ccagaatcac cacattcgct tcgagaatat catccccagc ctgcacgcca gtgaccttgt
     5401 ttccttcacg aaccagcgca tcgacgcgaa cgcctgggat aaactgcgcg cccgcctgct
     5461 cggcttgttc catcaaccac gggtccagac gattacgcaa tacggtataa gatgcgtgtt
     5521 gcggaacatc tggttgctcg cggtgaaaat cgagggtaac ggcgctttct tcggttaaga
     5581 aggagatttt ctcgcgtgtg accttgcgtt ctaccggcgc tgatactgca aagcctggaa
     5641 tgattgcttc aagtgtgtgg gcataaagac gcccgccggt catgttttta catccggcac
     5701 tgtcgccgcg ttctatcacc aggacatcca gcccggctcg cgccatgacc agtgcggcaa
     5761 cgctaccagc aacgcccgca ccgaccacaa tggcatcaaa tttgtcttcc gacatgcgtt
     5821 atgctccctt cccgctgcgt ttacagcggg agatgtggtt gaaaggggaa ttagcggctc
     5881 aactggctaa tcagggcagg gacgactttg tagatatcgc ccaccagacc gtagtcggca
     5941 taattgaaga ttggcgcatt tttatcttta ttgatggcga caatcacttt tgcgccgttg
     6001 ccgccaacca tatgctggat ctgcccggag atccccagcg tcaggtagag atcggatttc
     6061 agcaacacgc cggagacacc gatataacgt tcacgctcca tccagttctc gccttcggca
     6121 attggacgtg aacagccgac ttcagcattc agcaccgccg ccagttcgtg gaccattttc
     6181 aggtcatcct gcgccgccag accacgaccg acgccaacca cacgttttgc tttgctcagg
     6241 tccacgctgc ttgcggcttt cgcacggcgt tcgcgacaga gaatttcatg acgcggagca
     6301 acataaggta ccgtttctgt cgggcactga tgagaggtat cactggtgca cggttcctga
     6361 acaccgggtg caagggtaat gatcgccagc gggctgttga tcttttcctg agcgaacgct
     6421 aacccgccat acatccggtg ttcggcgcaa atgtgaccat cgacaatatc caccgccgtg
     6481 gcatcattca ccagcgccgc attcagttgc acacttaacc gtgctgccag agctttacca
     6541 cgtttcgtcg cggccaacag caacatagcg gggtgtttat ctttcagcag ggcagcaatg
     6601 ctttcggcgt aattttcagt gcgttgcagc gcgtcgtttt gctcaagaac ataaatacat
     6661 tttggaccat aaggcataac tgcctgcgcc tggtcggtat tttgtacaat ggcatacacc
     6721 tgttggcccc attgctgagc gccgccaaac agttcagcat aacgttcagg attatcgcta
     6781 aagacccaga cgctgtttaa ttgactcatc gtttcttctc cgtaggcttc agttcagggc
     6841 tttcttcaga tgttccgcca gctcggcaat ggcctccggc gaatcgttat cgataatgat
     6901 gtgcttacgt tctgtttgcg gcggtacgcg aatgccgacc agttcagcaa gtggcgcgct
     6961 ctggctccag tcaatatcac ttgcctgcca ctgatttacc ggttttttac ccgcgccgag
     7021 aatggctttc atcgaaggaa tgcgtggcac gttaatatcg gaggtgacgc agagcacggc
     7081 tggaacagag agttcaataa cttcaacatc atcttcaagc gtgcgttcaa tcaccagtgt
     7141 attgccctga cgctgaatag cactcactgc attaatcacc ggaagttgca gaatttctcc
     7201 gaccagcaag ccagcctgct gggcataaag gtcgccggaa ccttcaccaa agatcagtaa
     7261 atcgaagccg atcttttcaa ctgctgctgc cagcgctttt gcggtatcga gaggcagtgc
     7321 atgttcaagt tgcgcatcct gcaccaaata caggctgtgc ggcccgcggg atagcacgtc
     7381 tttgcgcact ttcgagttct gcaacaatga gccgccaacg gtcagcgcgg ctatctcatc
     7441 gtcatctgtt gcaagctggc ttgcagcttc aatggcattg agatcgaact ggctgatttt
     7501 ggcgtcggca ttgtcgaaat tcagggtgta ttctggagtg acaacaatgt cctgttcttc
     7561 aggcaccagc ttaaagcagg ttattatttt catgatttct cctgtgagtc gcatacggaa
     7621 ccatctccga tggaaggtaa aaatggcctg aaaatagtgt gcgatagcgg tgcggggttt
     7681 tcgacggagc aagctgtttt ccttgccaac aatcttgcaa aaaaaatgcg atcgctaaaa
     7741 caaaagtgca atttgcggca atatagttat tccatgaccg tacaatcttg cattcagtgt
     7801 aaataatctt gtttagctct cgctaaacgc ggttgtataa ccgcaataca cgccgtattt
     7861 tatccggcat atattgtttt tgcttgctca tggaataggg aggttgtcat gtatcaacgc
     7921 tgttttgata atgccagtga aacgctgttt gtcgccggta aaacgccacg gctttcacgt
     7981 tttgcaatta gcgatgatcc aaaatgggag tctggacatc acgttcatga caatgaaacc
     8041 gagctgattt acgtcaagaa aggggtagca aggtttacca tcgattcatc gttatatgtc
     8101 gcacatgccg atgatatagt ggtgatagaa cgcggcaggc tgcatgcggt ggcctctgac
     8161 gttaacgatc cggcaacgac gtgtacctgt gcgctgtacg gctttcagtt tcagggggct
     8221 gaggaaaatc agctactgca accgcattct tgtccggtaa ttgccgcggg gcagggaaaa
     8281 gaagtcataa aaaccttatt taatgagcta agtgtgattt tgccgcaaag taaaaatagc
     8341 caaacatctt cgttatggga tgcatttgcc tatacgttag ccattcttta ctacgaaaac
     8401 tttaaaaatg ct
//
LOCUS       DAZFQD010000186         8389 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.186, whole genome shotgun sequence.
ACCESSION   DAZFQD010000186 DAZFQD010000000
VERSION     DAZFQD010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8389
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.186"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            3..1226
                     /gene="lacY"
                     /locus_tag="ACHKTW_004688"
     CDS             3..1226
                     /gene="lacY"
                     /locus_tag="ACHKTW_004688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactose permease"
                     /protein_id="HFO6465577.1"
                     /translation="MFGLFFFFYFFIMGAYFPFFPIWLHDINHISKSDTGIIFAAISL
                     FSLLFQPLFGLLSDKLGLRKYLLWIITGMLVMFAPFFIFIFGPLLQYNILVGSIVGGI
                     YLGFCFNAGAPAVEAFIEKVSRRSNFEFGRARMFGCVGWALCASIVGIMFTINNQFVF
                     WLGSGCALILAVLLFFAKTDAPSSATVANAVGANHSAFSLKLALELFRQPKLWFLSLY
                     VIGVSCTYDVFDQQFANFFTSFFATGEQGTRVFGYVTTMGELLNASIMFFAPLIINRI
                     GGKNALLLAGTIMSVRIIGSSFATSALEVVILKTLHMFEVPFLLVGCFKYITSQFEVR
                     FSATIYLVCFCFFKQLAMIFMSVLAGNMYESIGFQGAYLVLGLVALGFTLISVFTLSG
                     PGPLSLLRRQVNEVA"
     gene            1292..1903
                     /gene="lacA"
                     /locus_tag="ACHKTW_004689"
     CDS             1292..1903
                     /gene="lacA"
                     /locus_tag="ACHKTW_004689"
                     /EC_number="2.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactoside O-acetyltransferase"
                     /protein_id="HFO6465578.1"
                     /translation="MNMPMTERIRAGKLFTDMCEGLPEKRLRGKTLMYEFNHSHPSEV
                     EKRESLIKEMFATVGENAWVEPPVYFSYGSNIHIGRNFYANFNLTIVDDYTVTIGDNV
                     LIAPNVTLSVTGHPVHHELRKNGEMYSFPITIGNNVWIGSHVVINPGVTIGDNSVIGA
                     GSIVTKDIPPNVVAAGVPCRVIREINDRDKHYYFKDYKVESSV"
     gene            complement(2006..3187)
                     /gene="cynX"
                     /locus_tag="ACHKTW_004690"
     CDS             complement(2006..3187)
                     /gene="cynX"
                     /locus_tag="ACHKTW_004690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyanate transporter CynX"
                     /protein_id="HFO6465579.1"
                     /translation="MRNHQKTFTMLLVLVLIGLNMRPLLTSVGPLLPQLRQASGMSFS
                     VAALLTALPVVTMGGLALAGSWLHQHVSERRSVAISLLLIAVGALMRELYPQSVLLLS
                     SALLGGVGIGIIQAVMPSVIKRRFQQRTPLVMGLWSAALMGGGGLGAAITPWLVQHSE
                     TWYQTLAWWALPAVVALFAWWWQSAREVASSHKTTTTPARVVFTPRAWTLGVYFGLIN
                     GGYASLIAWLPAFYIEIGASAQYSGSLLALMTLGQAAGALLMPAMARHQDRRKLLMLA
                     LVLQLVGFCGFIWLPLQLPVLWAMVCGLGLGGAFPLCLLLALDHSVQPAIAGKLVAFM
                     QGIGFIIAGLAPWFSGVLRSISGNYLMDWAFHALCVVGLMIITLRFAPVRFPQLWVKE
                     A"
     gene            complement(3193..3663)
                     /gene="cynS"
                     /locus_tag="ACHKTW_004691"
     CDS             complement(3193..3663)
                     /gene="cynS"
                     /locus_tag="ACHKTW_004691"
                     /EC_number="4.2.1.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008824 - cyanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009439 - cyanate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanase"
                     /protein_id="HFO6465580.1"
                     /translation="MIQSQINRNIRLDLADAILLSKAKKDLSFAEIADGTGLAEAFVT
                     AALLGQQALPADAARLVGAKLDLDEDAILLLQMIPLRGCIDDRIPTDPTMYRFYEMLQ
                     VYGTTLKALVHEKFGDGIISAINFKLDVKKVADPEGGERAVITLDGKYLPTKPF"
     gene            complement(3694..4353)
                     /gene="cynT"
                     /locus_tag="ACHKTW_004692"
     CDS             complement(3694..4353)
                     /gene="cynT"
                     /locus_tag="ACHKTW_004692"
                     /EC_number="4.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase CynT"
                     /protein_id="HFO6465581.1"
                     /translation="MKEIIDGFLKFQREAFPKREALFKQLATQQSPRTLFISCSDSRL
                     VPELVTQREPGDLFVIRNAGNIVPSYGPEPGGVSASVEYAVAALRVSDIVICGHSNCG
                     AMTAIASCQCMDHMPAVSHWLRYADSARVVNEARPHSDLPSKAAAMVRENVIAQLANL
                     QTHPSVRLALEEGRIALHGWVYDIESGSIAAFDGATRQFVPLAANPRVCAIPLRQPTA
                     A"
     gene            4463..5362
                     /gene="cynR"
                     /locus_tag="ACHKTW_004693"
     CDS             4463..5362
                     /gene="cynR"
                     /locus_tag="ACHKTW_004693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414872.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CynR"
                     /protein_id="HFO6465582.1"
                     /translation="MLSRHINYFLAVAEHGSFTRAASALHVSQPALSQQIRQLEESLG
                     VPLFDRSGRTIRLTDAGEVWRQYASRALQELGAGKRAIHDVADLTRGSLRIAVTPTFT
                     SYFIGPLMADFYARYPGITLQLQEMSQEKIEDLLCRDELDVGIAFAPVHSPELEAIPL
                     LTESLALVVAKHHPLAACEQVALSRLHDEKLVLLSAEFATREQIDHYCEKAGLHPQVV
                     IEANSISAVLELIRRTSLSTLLPAAIATQHDGLKAISLAPPLLERTAVLLRRKNSWQT
                     AAAKAFLHMALDKCAVVGGNESR"
     gene            complement(5699..6982)
                     /gene="codA"
                     /locus_tag="ACHKTW_004694"
     CDS             complement(5699..6982)
                     /gene="codA"
                     /locus_tag="ACHKTW_004694"
                     /EC_number="3.5.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine/isoguanine deaminase"
                     /protein_id="HFO6465583.1"
                     /translation="MSNNALQTIINARLPGEEGLWQIHLQDGKISAIDAQSGVMPITE
                     NSLDAEQGLVIPPFVEPHIHLDTTQTAGQPNWNQSGTLFEGIERWAERKALLTHDDVK
                     QRAWQTLKWQIANGIQHVRTHVDVSDATLTALKAMLEVKQEVAPWIDLQIVAFPQEGI
                     LSYPNGEALLEEALRLGADVVGAIPHFEFTREYGVESLHKTFALAQKYDRLIDVHCDE
                     IDDEQSRFVETVAALAHREGMGARVTASHTTAMHSYNGAYTSRLFRLLKMSGINFVAN
                     PLVNIHLQGRFDTYPKRRGITRVKEMLESGINVCFGHDDVFDPWYPLGTANMLQVLHM
                     GLHVCQLMGYGQINDGLNLITHHSARTLNLQDYGIAAGNSANLIILPAENGFDALRRQ
                     VPVRYSVRGGKVIASTQPAQTTVYLEQPEAIDYKR"
     gene            complement(6972..8231)
                     /gene="codB"
                     /locus_tag="ACHKTW_004695"
     CDS             complement(6972..8231)
                     /gene="codB"
                     /locus_tag="ACHKTW_004695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015209 - cytosine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HFO6465584.1"
                     /translation="MSQDNNFSQGPVPQSARKGVLALTFVMLGLTFFSASMWTGGTLG
                     TGLSYHDFFLAVLIGNLLLGIYTSFLGYIGAKTGLTTHLLARFSFGVKGSWLPSLLLG
                     GTQVGWFGVGVAMFAIPVGKATGLDINLLIAVSGLLMTVTVFFGISALTVLSVIAVPA
                     IACLGGYSVWLAVNGMGGLDALKAVVPAQPLDFNVALALVVGSFISAGTLTADFVRFG
                     RNAKLAVLVAMVAFFLGNSLMFIFGAAGAAALGMADISDVMIAQGLLLPAIVVLGLNI
                     WTTNDNALYASGLGFANITGMSSKTLSIINGIIGTVCALWLYNNFVGWLTFLSAAIPP
                     VGGVIIADYLMNRRRYEHFATTRMMSVNWVAILAVALGIAAGHWLPGIVPVNAVLGGA
                     LSYLILNPILNRKTTAAMTHVEANSVE"
BASE COUNT         2046 a         2229 c         2169 g         1945 t
ORIGIN      
        1 ggatgttcgg tttattcttt ttcttttact tttttatcat gggagcctac ttcccgtttt
       61 tcccgatttg gctacatgac atcaaccata tcagcaaaag tgatacgggt attatttttg
      121 ccgctatttc tctgttctcg ctattattcc aaccgctgtt tggtctgctt tctgacaaac
      181 tcgggctgcg caaatacctg ctgtggatta ttaccggcat gttagtgatg tttgcgccgt
      241 tctttatttt tatcttcggg ccactgttac aatacaacat tttagtagga tcgattgttg
      301 gtggtattta tctaggcttt tgttttaacg ccggtgcgcc agcagtagag gcatttattg
      361 agaaagtcag ccgtcgcagt aatttcgaat ttggtcgcgc gcggatgttt ggctgtgttg
      421 gctgggcgct gtgtgcctcg attgtcggca tcatgttcac catcaataat cagtttgttt
      481 tctggctggg ctctggctgt gcactcatcc tcgccgtttt actctttttc gccaaaacgg
      541 atgcgccctc ttctgccacg gttgccaatg cggtaggtgc caaccattcg gcatttagcc
      601 ttaagctggc actggaactg ttcagacagc caaaactgtg gtttttgtca ctgtatgtta
      661 ttggcgtttc ctgcacctac gatgtttttg accaacagtt tgctaatttc tttacttcgt
      721 tctttgctac cggtgaacag ggtacgcggg tatttggcta cgtaacgaca atgggcgaat
      781 tacttaacgc ctcgattatg ttctttgcgc cactgatcat taatcgcatc ggtgggaaaa
      841 acgccctgct gctggctggc actattatgt ctgtacgtat tattggctca tcgttcgcca
      901 cctcagcgct ggaagtggtt attctgaaaa cgctgcatat gtttgaagta ccgttcctgc
      961 tggtgggctg ctttaaatat attaccagcc agtttgaagt gcgtttttca gcgacgattt
     1021 atctggtctg tttctgcttc tttaagcaac tggcgatgat ttttatgtct gtactggcgg
     1081 gcaatatgta tgaaagcatc ggtttccagg gcgcttatct ggtgctgggt ctggtggcgc
     1141 tgggcttcac cttaatttcc gtgttcacgc ttagcggccc cggcccgctt tccctgctgc
     1201 gtcgtcaggt gaatgaagtc gcttaagcaa tcaatgtcgg atgcggcgcg agcgccttat
     1261 ccgaccaaca tatcataacg gagtgatcgc attgaacatg ccaatgaccg aaagaataag
     1321 agcaggcaag ctatttaccg atatgtgcga aggcttaccg gaaaaaagac ttcgtgggaa
     1381 aacgttaatg tatgagttta atcactcgca tccatcagaa gttgaaaaaa gagaaagcct
     1441 gattaaagaa atgtttgcca cggtagggga aaacgcctgg gtagaaccgc ctgtctattt
     1501 ctcttacggt tccaacatcc atataggccg caatttttat gcaaatttca atttaaccat
     1561 tgtcgatgac tacacggtaa caatcggtga taacgtactg attgcaccca acgttactct
     1621 ttccgttacg ggacaccctg tacaccatga attgagaaaa aacggcgaga tgtactcttt
     1681 tccgataacg attggcaata acgtctggat cggaagtcat gtggttatta atccaggcgt
     1741 caccatcggg gataattctg ttattggcgc gggtagtatc gtcacaaaag acattccacc
     1801 aaacgtcgtg gcggctggcg ttccttgtcg ggttattcgc gaaataaacg accgggataa
     1861 gcactattat ttcaaagatt ataaagttga atcgtcagtt taaattataa aaattgcctg
     1921 atacgctgcg cttatcaggc ctacaagttc agcgatctac attagccgca tccggcatga
     1981 acaaagcgca ggaacaagcg tcgcatcatg cctctttgac ccacagctgc ggaaaacgta
     2041 ctggtgcaaa acgcagggtt atgatcatca gcccaacgac gcacagcgca tgaaatgccc
     2101 agtccatcag gtaattgccg ctgatactac gcagcacgcc agaaaaccac ggggcaagcc
     2161 cggcgatgat aaaaccgatt ccctgcataa acgccaccag cttgccagca atagccggtt
     2221 gcacagagtg atcgagcgcc agcagcaaac agagcggaaa cgcgccgccc agacctaacc
     2281 cacacaccat cgcccacaat accggcaatt gcagcggcaa ccagataaag ccgcagaacc
     2341 ccaccagttg taacaccagc gccagcatta acagtttgcg ccgatcctga tggcgagcca
     2401 tagcaggcat cagcaaagct cctgcggctt gcccaagcgt catcaatgcc agtaaggaac
     2461 cgctgtactg cgcgctggca ccaatctcaa tatagaaagc gggtaaccag gcaatcaggc
     2521 tggcgtaacc gccgttaatc agaccgaagt aaacacccag cgtccacgcg cggggagtga
     2581 ataccacgcg agccggagtg gttgttgtct tgtgggaaga ggcgacctcg cgggcgcttt
     2641 gccaccacca ggcaaagagc gcaacaacgg caggcagcgc ccaccaggcg agtgtttgat
     2701 accaggtttc gctatgttga actaaccagg gcgttatggc ggcaccaagt ccaccgccgc
     2761 ccatcagagc cgcagaccac agccccatca ccagtggcgt gcgttgctga aaccgccgtt
     2821 taatcactga aggcatcacc gcctgaatga taccgatccc caccccacca agcagtgcgc
     2881 tgctaagcag cagcacactt tgggggtaaa gctcacgcat caatgcaccg acggcaatca
     2941 gcaacagact gatggcgacg ctgcgacgtt cgctgacatg ctgatgaagc cagcttccgg
     3001 ccagcgccag cccgcccatg gtgaccaccg gcagagcggt caacagggca gccacgctaa
     3061 agctcattcc gctcgcctgg cgcaattgcg gtagcagtgg accgacggag gtgagcagtg
     3121 gtcgcatatt aagaccaatc agcaccagaa ccagcagcat cgtgaacgtc ttttgatggt
     3181 tgcgcatggc tgtcagaacg gtttggtcgg cagatattta ccatctaagg tgatgaccgc
     3241 acgttcgcca ccttccgggt ccgccacttt cttaacgtcg agtttgaagt taatcgcgct
     3301 gataatgcca tcgccaaact tctcatgaac caacgctttc agggttgtgc cgtacacctg
     3361 caacatttca tagaaacgat acatcgttgg gtcggttgga atacggtcat caatgcagcc
     3421 acgcagtgga atcatctgca acagtagaat ggcgtcttcg tcgagatcca gcttcgcccc
     3481 gaccaggcgg gcggcgtcgg caggaagcgc ctgctgcccc agcaaagccg cggttacgaa
     3541 ggcttctgcc agaccggtac cgtcggcaat ctcggcaaat gagagatctt ttttagcttt
     3601 gctgagcaaa atggcatcgg caagatcaag acgaatattg cggttaattt gtgactgaat
     3661 catggtggat ctcctgatgg tttaaaaata aggttacgct gcggtcggtt ggcgtagcgg
     3721 tatggcacaa acgcgaggat tagcggccag tggcacaaac tggcgggttg cgccgtcaaa
     3781 agctgcgatg ctgccgcttt caatgtcgta gacccagccg tgcagggcga tccgcccctc
     3841 ttcgagcgcc aggcgcaccg atggatgagt ttgcaaatta gccaactgag caatgacgtt
     3901 ttcacgtacc atcgccgcag cttttgacgg taaatcggaa tgcgggcgcg cctcattaac
     3961 gacgcgggct gaatcggcat aacgcagcca gtgggagacg gcaggcatat ggtccataca
     4021 ctgacagctg gcaatggcgg tcatcgcgcc acagttggaa tgaccacaaa tcacaatgtc
     4081 agatacccga agcgcagcga cggcatactc caccgaagca gaaacgccac cgggttccgg
     4141 cccgtaggaa gggacaatat tacctgcgtt gcgaataacg aacagatcgc caggctcacg
     4201 ttgcgtcacc agctcaggga ccagacggct gtcggagcag gagataaaaa gggtgcgcgg
     4261 gctttgctgt gtcgccagct gtttaaataa ggcttcccgc ttcggaaatg cctcgcgctg
     4321 gaatttaagg aatccatcaa taatctcttt caccgttaac ctctgtctgt ctctggaatg
     4381 agaggcagac tactcgccga ttgtcataag gtaaaagtct catttatgat gagttctatt
     4441 ggatttactt ataggttggc gaatgctctc tcgacatatc aactattttc ttgccgtggc
     4501 tgaacatggc agctttaccc gtgccgccag tgcgttgcac gtctcccagc ctgcgctttc
     4561 ccagcagatt cgccagttag aggagagttt aggcgtgccg ctgtttgacc gtagcgggcg
     4621 aacgattcgt ctcactgatg caggagaagt ctggcgacag tacgccagcc gggcgttaca
     4681 ggaactggga gcgggtaaac gggcgattca tgatgttgcc gatctgacgc gaggatcgct
     4741 gcgtatcgcc gtcaccccca cctttacaag ctactttatc ggccctttaa tggcggattt
     4801 ctatgcgcgc taccccggca tcacgctcca gctacaggaa atgtcgcagg agaaaatcga
     4861 agatctgctt tgtcgcgacg agctggacgt tgggattgcc ttcgcgcctg tgcattcgcc
     4921 ggagctggag gcaattcctt tactgacaga aagtttagcg ttggtggtgg cgaaacatca
     4981 tccgctggct gcctgtgaac aggtggcgtt gagtcgcttg catgatgaaa aactggtcct
     5041 gctcagcgcg gaatttgcca ccagagagca aattgaccac tactgcgaga aagcggggct
     5101 acatccacag gtggtcattg aggcgaactc aattagcgcg gttctggagc tgattcgccg
     5161 cacttccctt tccacattgt taccagcagc gattgccaca caacatgacg ggcttaaagc
     5221 gatttctctt gccccgccac tactggagag aacggcggtt ttgttgcggc ggaaaaatag
     5281 ctggcagaca gccgccgcga aggcattttt gcacatggcg ttggataaat gcgcggttgt
     5341 tggcggaaat gaatcgcggt agccgctgac acaggtctgc ggctggaatt gcctgatgcg
     5401 ctacgcttat caggcctacg agttcagtgc tctacgtagg ccggataagg cgttcacgcc
     5461 gcatccggca taaactacag ctggcagact gccgctgcga aggcattttt gcacatggcg
     5521 ttggaggaat gcgcggatgt tggcggaaat gaatcacggt agccgctgac acagatcagc
     5581 ggctggaatt gcctgatgcg ctacgcttat caggcctacg agttcagtgc tctacgtagg
     5641 ccggataagg cgttcacgcc gcatccggca taaactacgc tcgctgtaac ccagccgatt
     5701 accgtttgta atcgatggct tctggctgct ccagatacac cgttgtttgt gctggttgtg
     5761 tactggcaat caccttgccg ccacgtaccg aataacgtac cggaacctga cggcgcagcg
     5821 catcaaaccc attttcagcc ggcaggataa tcaggttggc gctgtttccg gcggcaatgc
     5881 cgtaatcctg caaattcaac gtccttgcgc tgtggtgggt gattaaattc aggccatcgt
     5941 taatctgccc gtagcccatc aactggcaaa catgcagccc catatgcagc acttgcagca
     6001 tattcgccgt tcccagcgga taccacggat cgaagacatc atcgtgacca aagcagacgt
     6061 taatgccgga ctccagcatc tctttaacgc gcgtgatgcc gcgacgtttt ggatacgtat
     6121 cgaaacgtcc ttgcagatga atattgacca gcgggttggc gacaaagtta ataccggaca
     6181 ttttcagcaa gcggaacagg cgtgaggtat acgccccgtt ataggagtgc attgccgtgg
     6241 tgtggctggc ggtgactcgc gcgcccatgc cttcacggtg cgccagggca gcaacggttt
     6301 cgacaaagcg cgactgctcg tcatcgatct catcacagtg aacgtcgatg agacggtcgt
     6361 atttttgcgc cagggcgaag gttttatgca gcgactccac gccgtattca cgggtaaatt
     6421 caaaatgcgg aatcgccccc actacatctg cccctaagcg taacgcctct tccagcaacg
     6481 cttcaccgtt gggatacgac aaaatccctt cctgcgggaa ggcgacgatt tgcagatcaa
     6541 tccacggcgc gacttcctgc ttcacttcca gcattgcttt cagcgcagtc agcgttgcat
     6601 ccgaaacatc gacatgggta cgcacatgct gaatgccgtt ggcaatctgc catttcagcg
     6661 tttgccaggc gcgttgtttc acatcgtcat gggttaataa cgctttgcgt tcggcccagc
     6721 gttcaatgcc ttcaaacagc gtgccggact gattccagtt cggttgtccg gcggtttgcg
     6781 tggtgtccag gtgaatatgc ggctccacaa acggcggtat aactaaacct tgttcggcat
     6841 ccaggctgtt ttcagttatg ggcatcacgc cggattgcgc atcaatggcg ctgatttttc
     6901 cgtcctgcag atgaatctgc cacagcccct cttcgcctgg taaccgggcg ttaataattg
     6961 tttgtaaagc gttattcgac actgttagcc tccacatgcg tcattgctgc tgtcgtttta
     7021 cgattcaaaa tcgggttaag gatcagatag ctcagcgcgc cacctaatac cgcgttgacc
     7081 ggaacaattc ccggtaacca gtggcctgca gcaatcccca aggcgaccgc cagaatcgcc
     7141 acccaattga cactcatcat acgcgtggtc gcaaagtgct catagcggcg acggttcatc
     7201 agatagtcgg cgatgatcac gccacccact ggaggaatag ctgccgaaag gaaggtcaac
     7261 cagccgacaa aattgttata cagccataat gcgcagaccg taccgataat accgttgatt
     7321 atcgaaaggg ttttgctcga catcccggta atgttggcga aacctaaacc cgacgcatag
     7381 agtgcgttat cgttggtggt ccagatattc agccccagca ccacaatcgc aggcagcagc
     7441 aggccctgag caatcatcac atcagagata tccgccatgc ccagtgccgc agcgcccgct
     7501 gcaccgaaaa taaacatcaa cgagttgccg aggaaaaagg ccaccatcgc caccagcacc
     7561 gccagtttgg cattgcgacc aaaccggaca aagtcagcgg tgagcgtacc cgcactgata
     7621 aatgacccca caaccagcgc cagcgcgaca ttgaaatcta acggttgtgc gggaacgacc
     7681 gcttttaatg cgtccaggcc gcccatgccg ttaacagcca gccacacgga ataaccgccc
     7741 aggcaggcga tagccggaac cgcaatcacc gaaagaaccg tcagcgccga aatgccaaaa
     7801 aagacggtga cggtcatcag taaaccggaa acggcaatca gcaaattaat atccagcccg
     7861 gttgccttac ccaccggaat ggcaaacatc gccacaccga cgccaaacca gccaacctga
     7921 gttccgccca gtagcagtga aggcagccat gagcctttaa caccaaacga gaagcgagca
     7981 agaagatgag tggtcaggcc ggtttttgcg ccaatgtaac cgagaaatga agtgtaaata
     8041 ccgaggagaa gattaccgat gagaactgcg aggaagaaat catgatagct aagaccggtt
     8101 ccgagagtgc cgccggtcca catactggcg gaaaagaagg ttaatcccag catgacgaac
     8161 gtcaatgcca ataccccttt ccgcgccgac tgcgggactg gcccctggct aaagttgtta
     8221 tcttgcgaca cgaaattccc cctttttgct gtttgaaacc caaaaaatcc gccgcattct
     8281 attcatctga agataaaaag caatcgtttt cgtggggaaa tatatttttt atatgaatga
     8341 gtgcaaaaag gtgggggaaa aattatcggt gtaaaaacag tgggttgtg
//
LOCUS       DAZFQD010000187         8112 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.187, whole genome shotgun sequence.
ACCESSION   DAZFQD010000187 DAZFQD010000000
VERSION     DAZFQD010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8112
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.187"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            330..788
                     /locus_tag="ACHKTW_004696"
     CDS             330..788
                     /locus_tag="ACHKTW_004696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HFO6465585.1"
                     /translation="MPTPCYISITGQTQGNITAGAFTADSVGNIYVQGHEDEMLVQEF
                     LHNVTVPTDPQSGQPAGQRAHKPFIFTVALNKAVPLMYNALASGEMLPTTELHWWRTS
                     VEGKQEHYFTTRLTDSTIVDMKLHMPHCQDPAKREFTQLLEVSLAYRKIE"
     gene            1058..3199
                     /locus_tag="ACHKTW_004697"
     CDS             1058..3199
                     /locus_tag="ACHKTW_004697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HFO6465586.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLDLSLVSQQ
                     FLSLEFQQILDKMAYLTIWQGDDVQRRVKGMVTWFELGENDKNQMLYSMKVCPPLWRT
                     GLRQNFRIFQNEDIESILGTILQENGVTEWSPLFSEPHPSREFCVQYGETDYDFLCRM
                     AAEEGIFFYEEHAQKSTDHSLVLCDTVRYLPESFEIPWNPNTRTEVSTLCISQFRYSA
                     QIRPSSVVTKDYTFKRPGWAGRFDQEGQYQDYQRTQYEVYDYPGRFKGAHGQNFARWQ
                     MDGWRNNAEVARGTSRSPEIWPGRRIVLTGHPQANLNREWQVVASDLHGEQPQAVPGR
                     RGSGTTQDNHFAVIPADRTWRPQPLLKPLVDGPQSAVVTGPAGEEIFCDEHGRVRVKF
                     NWDRYNPSNQESSCWIRVAQAWAGTGFGNLAIPRVGQEVIVDFLNGDPDQPIIMGRTY
                     HHENRTPGSLPGTKTQMTIRSKTYKGSGFNELKFDDATGKEQVYIHAQKNMNTEVLNN
                     RTTDVINNHAEKIGNNQAITVTNNQIQNIGVNQIQTVGVNQVETVGSNQIIKVGSNQV
                     EKVGIIRALTVGVAYQTTVGGIMNTSVALLQSSQVGLHKSLMVGMGYSVNVGNNVTFS
                     VGKTMKENTGQTAVYSAGEHLELCCGKARLVLTKDGSIFLNGTHIHLEGESDVNGDAP
                     VINWNCGATQPVPDAPVPKDLPPGMPDMRQF"
     gene            3275..7528
                     /locus_tag="ACHKTW_004698"
     CDS             3275..7528
                     /locus_tag="ACHKTW_004698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element core protein"
                     /protein_id="HFO6465587.1"
                     /translation="MGGKPAARQGDMTRKGLDIVQGSAGVLIGAPTGVACSVCPGGIT
                     YANPVNPLLGAKVLPGETDLALPGPLPFILSRAYSSYRTRTPAPVGVFGPGWKAPFDI
                     RLQIRDEGLILNDSGGRSIHFEPLFPGEISYSRSESLWLARGGVAAQYSSQPLSALWQ
                     VLPEDVRLSPHVYLATNSLQGPWWILSWPERVPGADEVLPPEPPAYRVLTGVVDGFGR
                     TLAFHRAAKGDVAGAVTGVTDGAGRRFHLALTTQAQRAEAFRKQRATSLSSPAGPRSA
                     SSSSAFPDTLPAGTEYGADNGIRLEAVWLTHDPAYPDEQPTAPLARYTYTASGELRAV
                     YDRSGTQVRGFTYDAEHAGRMVAHHYAGRPESRYRYDDTGRVTEQVNPEGLDYRFEYG
                     QDRVTITDSLNRREVLYTEGEGGLKRVVKKEHADGSITRSEYDEAGRLKAQTDAAGRR
                     TEYRLHMASGAVTAVTGPDGRTVRYGYNSQRQVTSVTYPDGLRSSREYDERGRLTAET
                     SRSGETTRYSYDDPASELPTGIQDATGSTKQMAWSRYGQLLAFTDCSGYTTRYEYDRY
                     GQQIAVHREEGISTYSSYNPRGQLVSQKDAQGREIRYEYSAAGDLTATVSPDGKRSTI
                     EYDKRGRPVSVTEGGLTRSMGYDAAGRITVLTNENGSQSTFRYDPVDRLTEQRGFDGR
                     TQRYQYDLTGKLTQSEDEGLITLWHYDASDRITRRTVNGEPAEQWQYDDHGWLTEISH
                     LSEGHRVAVHYGYDDKGRLTGERQTVENPETGEMLWEHETGHAYSEQGLATRQEPDGL
                     PPVEWLTYGSGYLAGMKLGGTPLVEYTRDRLHRETARSFGGAGSTAGYEQATAYTLTG
                     QLQSRHLNLPQLDCDYTWNDNGQLVRISGPQECREYRYSGTGRLTGVHTTAANLDIDI
                     PYATDPAGNRLPDPELHPDSTLTAWPDNRIAEDAHYVYRYDEYGRLAEKTDRIPEGVI
                     RMHDERTHHYHYDSQHRLVFYTRIQHGEPQVESRYLYDPLGRRTGKRVWRRERDLTGW
                     MSLSRKPEETWYGWDGDRLTTVQTQQTRIQTVYQPGSFTPLLRIETENGEQAKARHRS
                     LAEVLQEDTGVTLPAELAVMLGRLERELRQGSVSEESQQWLAQCGLTAEQMAAQLEAE
                     YIPERKLHLYHCDHRGLPLALISPEGETAWQGEYDEWGNLLGEESAQHLQQSLRLPGQ
                     QYDEESGLYYNRNRYYDPLQGRYITQDPIGLRGEWNLYKYPLNPVRFIDSLGLKFHVN
                     GDPSDFNQAVEYLKQDSQMKETIDFLSSSEETINIEYIEGTNVRFNSNNMAIYWNSRA
                     SLFCSTELNSKSQSPALGLGHEFAHAQYYLLDKENFMALLSRTDKKYENKEEARVITI
                     IESRAAKTLGECTRGAHSGLPFYRVDGPLQTMKITGTPE"
     gene            7540..7989
                     /locus_tag="ACHKTW_004699"
     CDS             7540..7989
                     /locus_tag="ACHKTW_004699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000978828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein dsORF-h1"
                     /protein_id="HFO6465588.1"
                     /translation="MMKYLMVLLSLFSGSVLGMGRVNELCGIDSVKTIEIINLPSYVT
                     TLVPLSKEGLNEIYRYKVVVNEISDLYAGKIIDLLQMKYFRKEKYNNIRWGVSIISKG
                     NNKCEIYFDAFGECGSVNGINVCFEKNEMIGWIKKEIPLLSQKIGGL"
BASE COUNT         2005 a         1959 c         2538 g         1610 t
ORIGIN      
        1 ggaatatctc aagaattatc aaaatgataa ttcagaaagg ataaatacaa tatttattca
       61 tgattcagct ttggccattt tatggaggca atacggactt tgaacagttg ggtatgatta
      121 tccagatata tattattaag ttattgctga ataagagatt taatgatttt aaaaatagtt
      181 tttacgacaa ccgtaaattc tagctcggtt tgtatgtaaa aaatactgaa aaatgatgcg
      241 gttccgtgaa gtgagctgca tctgcctgtc tgggattaac ggcaggtcat tgtaagtagt
      301 aattaaccac gttaacaagg agtttacaaa tgccaacacc gtgttatatc tccatcactg
      361 gtcagaccca gggaaatatt actgcaggtg catttaccgc tgattctgtc ggcaacatct
      421 atgtgcaggg gcacgaagac gagatgctgg tgcaggaatt cctgcataac gttactgtcc
      481 cgaccgatcc tcagtccggt cagcctgcgg gtcagcgtgc tcacaagcct ttcattttta
      541 ccgtggcgct gaacaaagcg gttccgctga tgtacaacgc actggcctcc ggcgaaatgc
      601 tgccaaccac tgaacttcac tggtggcgta cctctgtgga aggcaaacag gagcattatt
      661 tcactacccg cctgactgat tcgacgattg tcgatatgaa actgcatatg ccgcattgcc
      721 aggacccggc gaaacgcgag ttcacccagt tgctcgaagt ttcactggcc taccgcaaaa
      781 ttgaatagga acacgtcaaa tctggcacct ccggtgccga cgactggcgc gcaccgctgg
      841 aagcataagt taagccaaca gcatccggct atcaggccgg atgctgtttt tgtattacgc
      901 cgtagaaaga aaggttgtca gtaaggggtt acgttcacgg gagatggcgg ctgccggaaa
      961 cttcggcggt agcgtgcctg aacgcaagcg taacactgta ctgtcacagc cgctttaaca
     1021 gcgaactgtc tgtttttcct taaccccgga ggttgctatg tcaaccggat tacgtttcac
     1081 gctggaagtg gacggcctgc caccggatgc ttttgcggtg gtctcctttc atctgaacca
     1141 gtcactctct tcgctttttt ccctcgatct ctctctggtc agccagcagt ttctctccct
     1201 tgaattccag cagatcctcg acaaaatggc ctacctgacg atatggcagg gcgatgacgt
     1261 acagcgccgg gtgaaaggta tggtgacctg gtttgaactg ggggagaacg acaaaaacca
     1321 gatgctgtac agcatgaagg tgtgcccacc gctgtggcgc acagggctgc gccagaactt
     1381 ccgtatcttc cagaatgagg acatcgaaag catcctcggc acgatattgc aggaaaacgg
     1441 ggtgaccgag tggagcccgc tgttcagcga gccacatcct tcccgtgagt tttgtgtcca
     1501 gtatggtgag actgattacg atttcctgtg ccggatggcg gcggaggaag gcatcttctt
     1561 ttatgaggag cacgcgcaaa aaagtaccga ccacagcctg gtcctgtgcg ataccgtgcg
     1621 ttatctgccg gagtcctttg agatcccctg gaatccgaac acccgtaccg aggtgagcac
     1681 cctctgcatc agccagtttc gctacagcgc acaaatccgc ccttcttccg tggtgaccaa
     1741 agactacacc tttaaacgac ccggctgggc agggcgtttt gatcaggaag gccagtacca
     1801 ggattaccag cgcacacagt atgaagtgta tgactacccc ggacgtttca agggtgccca
     1861 cgggcagaac tttgcccgct ggcagatgga tggctggcgc aacaacgcag aagtggcgcg
     1921 cggaacaagc cgttcgccgg agatatggcc gggacggcga attgtgctga cggggcatcc
     1981 gcaggcgaac ctgaaccggg aatggcaggt ggtggcgagc gatcttcacg gcgaacagcc
     2041 gcaggcggta ccgggacgca ggggttcagg caccacgcag gataaccact ttgcggtaat
     2101 cccggcagac cggacatggc ggccacagcc gttgctgaaa ccgctggtgg acggcccgca
     2161 gagcgccgtc gtgacgggac cggcaggcga ggaaatcttc tgtgatgaac atggccgcgt
     2221 gcgggtgaaa tttaactggg accgctataa cccgtcaaac caggaaagtt catgctggat
     2281 ccgtgtggca caggcgtggg caggcaccgg ctttggtaac ctggcgatac cgcgtgtggg
     2341 tcaggaggtc attgtggact tcctcaacgg cgacccggac cagccgatta ttatggggcg
     2401 tacctaccac catgaaaacc gcacacccgg cagcctgccg gggacgaaga cgcagatgac
     2461 gatccgctcg aaaacctata agggcagcgg gtttaatgaa ctgaagtttg acgatgcgac
     2521 agggaaagaa caggtctaca tccacgcgca gaagaacatg aacaccgagg tgctgaataa
     2581 ccgcaccact gatgtgataa acaaccatgc cgaaaaaata ggtaacaacc aggcgatcac
     2641 cgttaccaat aaccagatcc agaacattgg cgttaatcag atacagacgg ttggtgtcaa
     2701 ccaggtggaa acggtgggca gtaaccagat tatcaaagtg ggatcaaacc aggttgaaaa
     2761 ggtggggatc attcgtgcgc tgacggtggg tgtggcttac cagacgacgg taggcggcat
     2821 tatgaatacc tcggtggcgt tgttgcagtc ctcacaggta gggctgcata aatcactgat
     2881 ggtgggaatg ggctacagcg tcaatgtggg gaataacgtc accttctcgg tgggcaagac
     2941 aatgaaggaa aacaccggac aaacagcagt ttattctgcc ggtgagcatc ttgaactctg
     3001 ctgtggtaag gcaaggctgg tgctgacgaa ggacggaagc atatttctca atggtacgca
     3061 cattcatctg gaaggggagt cggatgtgaa cggtgatgcg ccagtgatta actggaactg
     3121 tggtgccaca caacctgtac cggatgcgcc tgtgccgaaa gatttacccc ccggaatgcc
     3181 ggatatgcgg caattttgag tcaggtgcaa aaaattaaaa cggaactggt gcagtgacca
     3241 gataaaccat attaaaagca gaaggaattg attcatgggc ggaaaaccgg cggcgcggca
     3301 gggtgatatg acccgcaagg gactggatat tgtgcagggt tcagcggggg tgctgatagg
     3361 tgcgccgacg ggagtggcct gttcggtatg tccgggaggg attacctatg ctaacccggt
     3421 gaacccgctg ctgggtgcga aggtgctgcc gggcgagacg gaccttgcgc tgcccggccc
     3481 gctgccgttt attctttccc gcgcctacag cagctaccgg accagaacac ccgcgccggt
     3541 gggggtgttt ggtcccggct ggaaagcgcc cttcgatatc cgcttacaga tacgcgatga
     3601 aggcctgata ctcaacgaca gcggcggcag gagcattcac tttgagccgc tgtttcccgg
     3661 cgagataagc tacagccgca gcgagtcact ctggctggcg cggggcggag tagcggcgca
     3721 gtacagttcg cagccgctaa gcgcgctctg gcaggtgctg ccggaagatg ttcgcctgag
     3781 tccgcatgtg tacctggcga caaacagcct tcaggggccg tggtggatac tcagctggcc
     3841 ggagcgggtg ccgggggcgg acgaggtgct gccgccggag ccgcccgcat accgggtgct
     3901 gacgggcgtg gtggacggct tcgggcggac gctggccttt caccgggcag ctaagggtga
     3961 cgtggcgggc gcggtgacgg gggtgacgga cggcgcgggc cgccgttttc acctggcgct
     4021 gaccacgcag gcgcagcggg cggaagcctt ccgtaaacag cgcgccacgt ctttatcttc
     4081 ccctgccggt ccccgttctg cttcctcttc ttctgctttc cccgacacgc tgcccgccgg
     4141 tacagaatac ggtgccgata acggtatccg gctggaggcg gtgtggctga cacacgaccc
     4201 ggcatacccg gatgaacagc cgactgcgcc gctggcgcgc tacacgtaca cggccagcgg
     4261 agaactgcgg gcggtgtatg accgcagcgg aacgcaggtg cgcgggttta cttatgatgc
     4321 ggagcacgcc gggcggatgg tggcgcacca ttatgcaggg agaccggaga gccgctaccg
     4381 gtatgatgat accgggcggg tgacggagca ggtcaacccg gaggggctgg actatcgctt
     4441 tgagtacggg caggaccgtg tgaccatcac ggacagcctg aaccggcggg aggtgctgta
     4501 cacggagggc gaaggcggcc tgaagcgggt ggtgaagaag gagcatgccg acgggagcat
     4561 cacccgcagc gagtatgatg aggcggggag gctgaaggcg cagacggatg cggcggggcg
     4621 gcggacggag taccggctgc atatggcgtc gggcgcggtg acagcggtga cggggccgga
     4681 cggcaggacg gtgcggtatg gctataacag ccagcggcag gtgacgtcag tgacgtaccc
     4741 ggacgggctt cgcagcagcc gggagtatga tgagagggga aggctgacgg cggagacctc
     4801 gcgcagcggt gagacaacgc ggtacagcta tgatgacccg gcgagtgagc tgccgacagg
     4861 gatacaggac gcgacgggca gtacaaaaca gatggcatgg agccgttacg gtcaactgct
     4921 ggccttcacg gactgctcgg ggtacacgac gcggtatgag tacgaccggt acggtcaaca
     4981 aatcgccgtt caccgggagg aaggcatcag cacttacagc agttataacc cgcgtgggca
     5041 actggtcagt cagaaggatg cgcaggggcg tgaaatccgt tatgagtaca gcgccgcagg
     5101 cgacctgaca gcaacggtat caccggacgg taaacgcagc accatcgaat atgataaacg
     5161 cgggcgtccg gtgagtgtga cggaaggcgg tctgacgcgc agcatggggt atgacgctgc
     5221 cgggcgcatc actgtgctga ccaacgagaa cggcagccag tccacgttcc ggtatgaccc
     5281 ggtcgacagg ctgacggagc agcgcggctt tgacggccgg acgcaacgtt accagtatga
     5341 cctgaccgga aaactcacgc agagtgaaga tgaggggctt atcaccctct ggcattacga
     5401 tgcgtcggac cgtatcaccc ggcgcacggt gaacggtgaa ccggcggagc agtggcagta
     5461 tgacgaccac ggctggctga cagaaatcag ccacctgagt gaaggccacc gtgtggcggt
     5521 ccactacggc tatgacgata aaggccgtct gacgggtgaa cggcagacgg tggagaaccc
     5581 ggagacaggg gagatgctgt gggagcatga gacggggcat gcgtacagcg aacaggggct
     5641 ggcgacccgt caggagccgg acggtctgcc gccggtggag tggctgacgt atggcagcgg
     5701 ttatcttgcg gggatgaagc tgggcggaac gccgctggtc gagtacacgc gggaccggct
     5761 gcaccgcgag acggcccgta gcttcggcgg ggcgggcagt acagcggggt acgaacaggc
     5821 cacggcgtac accctcacag ggcagctaca gagcaggcac ctgaaccttc cgcagcttga
     5881 ctgtgactat acgtggaacg ataacggaca actggtgcgc atcagcggcc cgcaggagtg
     5941 ccgggagtac cgttacagcg gcacgggaag gctgacgggc gtccacacta cggcagcgaa
     6001 cctggatatc gatatcccgt atgcgacgga cccggcagga aaccggctgc cggacccgga
     6061 actgcacccg gacagcaccc tcacggcgtg gccggataac cgcatcgcgg aagatgcgca
     6121 ctatgtctac cgctacgatg aatacggcag gctggcggag aagacggacc gcatcccgga
     6181 aggggtgata cggatgcacg acgagcggac ccaccactac cactacgaca gccagcaccg
     6241 tctggtgttc tacacgcgga tacagcacgg tgagccgcag gtggagagcc gctacctcta
     6301 tgacccgctg ggccgccgga cgggaaaacg ggtgtggcgg cgggagcgtg acctgacggg
     6361 gtggatgtcg ctgtcgcgta aaccggagga gacctggtac gggtgggacg gtgacaggct
     6421 gaccactgta cagacccaac agacaagaat ccagacggta taccagccgg gaagcttcac
     6481 gccgctcctg agaatcgaaa cagagaatgg tgaacaggcg aaggcgcggc accgtagtct
     6541 ggcggaggtg ttgcaggagg acacgggtgt gacgctaccg gcggagctgg cggtgatgct
     6601 ggggaggctg gagcgggaac tgcggcaggg cagcgtgagt gaagaaagcc agcagtggct
     6661 tgcgcagtgc gggctgacgg cggaacagat ggccgcgcaa ctggaggcgg aatacatccc
     6721 ggagaggaaa cttcatcttt accactgtga ccaccgtggg ctgccgctgg cgcttatcag
     6781 cccggaaggg gaaacggcgt ggcaggggga gtatgacgag tggggaaacc tgctgggcga
     6841 ggaaagcgcg cagcaccttc agcagtcact ccgtctgccg ggacagcagt atgatgagga
     6901 gtcggggctg tactacaacc gcaaccggta ctatgatccg ttgcagggga gatatatcac
     6961 ccaggacccg atagggctga gaggtgaatg gaatctgtat aaatatccat taaaccctgt
     7021 tcggtttata gattctttgg gtttaaaatt ccatgttaac ggtgatccaa gtgattttaa
     7081 tcaggctgtt gagtatttaa aacaagattc acaaatgaaa gaaactatag attttttatc
     7141 aagctctgaa gaaactataa atatagagta tattgaagga accaatgtta gatttaattc
     7201 gaataatatg gcgatatatt ggaatagtag agcatcttta ttttgttcaa ctgaattaaa
     7261 ctcgaaatca caatcgcctg cattaggttt gggacatgag tttgctcatg cgcagtatta
     7321 ccttcttgat aaggaaaatt tcatggctct tttaagccga acagataaga aatatgagaa
     7381 taaagaagag gcaagggtta ttacaataat tgaatcgcgt gcggcgaaaa ctttaggtga
     7441 gtgtaccaga ggagctcata gtggactgcc tttttatcgg gtggatggac cgttgcagac
     7501 gatgaaaata acgggtacgc cagaatagtg agatttttta tgatgaaata tttgatggtt
     7561 ttattgtctc tattttctgg ttcggtactg gggatggggc gagtgaatga attgtgtgga
     7621 atagatagcg tgaaaacgat agagataatt aatcttccat cgtatgttac cacgttagtt
     7681 ccgcttagca aggaaggtct taatgaaata tacagatata aagtggtggt gaatgaaatt
     7741 agcgatcttt atgctggtaa gataattgat ttattgcaaa tgaagtattt cagaaaagaa
     7801 aaatataaca atataagatg gggggttagt atcatttcga aaggtaataa taaatgtgaa
     7861 atttattttg atgcttttgg tgaatgtgga agtgtgaatg gcattaacgt gtgttttgaa
     7921 aaaaatgaaa tgataggttg gattaaaaaa gaaatccctt tgctgtcgca aaaaataggt
     7981 gggctttaaa tggtagggca atattcaaag acagtatagg taactatttg tatggccatg
     8041 taaggtaaaa gaaaaggaat aaaccatgag cggaaaaccg gcggcgcggc agggcgacat
     8101 gacccgcagg ga
//
LOCUS       DAZFQD010000188         8107 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.188, whole genome shotgun sequence.
ACCESSION   DAZFQD010000188 DAZFQD010000000
VERSION     DAZFQD010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8107
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.188"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            135..1268
                     /gene="ompN"
                     /locus_tag="ACHKTW_004700"
     CDS             135..1268
                     /gene="ompN"
                     /locus_tag="ACHKTW_004700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
                     /protein_id="HFO6465589.1"
                     /translation="MKSKVLALLIPALLAAGAAHAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNSAKDGDQSYARLGFKGETQINDQLTGYGQWEYNIQANNTESSKNQSWTRLAFAGL
                     KFADYGSFDYGRNYGVMYDIEGWTDMLPEFGGDSYTNADNFMTGRANGVATYRNTDFF
                     GLINGLNFAVQYQGNNEGASNGQEGTNNGRDVRHENGDGWGLSTTYDLGMGFSAGAAY
                     TSSDRTNDQVNHTAAGGDKADAWTAGLKYDANNIYLATMYSETRNMTPFGDSDYAVAN
                     KTQNFEVTAQYQFDFGLRPAVSFLMSKGRDLHAAGGADNPAGVDDKDLVKYADVGATY
                     YFNKNMSTYVDYKINLLDEDDSFYAANGISTDDIVALGLVYQF"
     gene            1409..1843
                     /gene="uspF"
                     /locus_tag="ACHKTW_004701"
     CDS             1409..1843
                     /gene="uspF"
                     /locus_tag="ACHKTW_004701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HFO6465590.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEM
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            2019..2954
                     /gene="ttcA"
                     /locus_tag="ACHKTW_004702"
     CDS             2019..2954
                     /gene="ttcA"
                     /locus_tag="ACHKTW_004702"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HFO6465591.1"
                     /translation="MSQNQEISKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(3083..4455)
                     /gene="dbpA"
                     /locus_tag="ACHKTW_004703"
                     /pseudo
     CDS             complement(3083..4455)
                     /gene="dbpA"
                     /locus_tag="ACHKTW_004703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309954.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
     gene            complement(4485..4658)
                     /gene="ynaL"
                     /locus_tag="ACHKTW_004704"
     CDS             complement(4485..4658)
                     /gene="ynaL"
                     /locus_tag="ACHKTW_004704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaL"
                     /protein_id="HFO6465592.1"
                     /translation="MTTLIYLQIPVPEPIPGDPVPVPDPIPRPQPMPDPPPDEEPIKL
                     SHRERRSARIRAC"
     gene            complement(4933..5916)
                     /gene="zntB"
                     /locus_tag="ACHKTW_004705"
     CDS             complement(4933..5916)
                     /gene="zntB"
                     /locus_tag="ACHKTW_004705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0071577 - zinc ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HFO6465593.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRLGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            complement(5930..5977)
                     /gene="ydaN"
                     /locus_tag="ACHKTW_004706"
     CDS             complement(5930..5977)
                     /gene="ydaN"
                     /locus_tag="ACHKTW_004706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaN"
                     /protein_id="HFO6465594.1"
                     /translation="MLRNRARRLDYKIMR"
     gene            6171..7403
                     /gene="dgcM"
                     /locus_tag="ACHKTW_004707"
     CDS             6171..7403
                     /gene="dgcM"
                     /locus_tag="ACHKTW_004707"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
                     /protein_id="HFO6465595.1"
                     /translation="MITHNFNTLDLLTSPVWIVSPFEEQLIYANSAARLLMQDLTFSQ
                     LRTGPYSVSSQKELPKYLSDLQNQHDIIEILTVQRKEEETALSCRLVLRELTETEPVI
                     IFEGIEAPATLGLKASRSANYQRKKQGFYARFFLTNSAPMLLIDPSRDGQIVDANLAA
                     LNFYGYNHETMCQKHTWEINMLGRRVMPIMHEISHLPGGHKPLNFVHKLADGSTRHVQ
                     TYAGPIEIYGDKLMLCIVHDITEQKRLEEQLEHAAHHDAMTGLLNRRQFYHITEPGQM
                     QHLAIAQDYSLLLIDTDRFKHINDLYGHSKGDEVLCALARTLESCARKGDLVFRWGGE
                     EFVLLLPRTPLDTALSLAETIRVSVAKVSISGLPRFTVSIGVAHHEGNESIDELFKRV
                     DDALYRAKNDGRNRVLAA"
     gene            complement(7424..7987)
                     /gene="smrA"
                     /locus_tag="ACHKTW_004708"
     CDS             complement(7424..7987)
                     /gene="smrA"
                     /locus_tag="ACHKTW_004708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HFO6465596.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLSQPLEFRREGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMM
                     FSFIQQAMADGLRNVLIIHGKGRDDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKTAQAKQENWERHAKRSR"
BASE COUNT         2146 a         2024 c         1913 g         2024 t
ORIGIN      
        1 ggaattattt gattaaggtt ttacttaagg cgtaacaaat gatatttatc ggctaactga
       61 acttctcctt taggatgttt tcactccccg aaatggggat aaaaggcaaa taaaataact
      121 aaaggattta ttcaatgaaa agcaaagtac tggcactttt aattcctgcc ctgctcgccg
      181 caggtgctgc acatgcagcc gaagtttata ataaagacgg caacaaatta gatctgtatg
      241 gcaaagttga tggcctgcat tatttttctg ataattcggc gaaagatggc gaccagagct
      301 atgcgcgtct gggttttaaa ggcgaaaccc aaattaacga tcaactcact ggctacggtc
      361 aatgggaata caatattcag gcaaacaaca ctgaatcttc aaaaaaccag tcatggaccc
      421 gtctggcatt tgccgggctg aaatttgcag attacggttc tttcgattac ggacgtaatt
      481 atggcgtaat gtacgacatc gaaggctgga ccgatatgct gcctgaattt ggcggtgact
      541 cttataccaa tgcagacaac tttatgactg gtcgagccaa tggcgtcgcg acttatcgta
      601 atactgattt cttcggtctg ataaatggtc tgaacttcgc ggtgcagtat caaggtaaca
      661 acgaaggtgc cagtaatggt caggaaggca ccaacaacgg acgtgatgtt cgccatgaaa
      721 acggtgacgg ctggggtctt tccacaacat atgatttagg catgggcttt agcgctggtg
      781 cggcatacac ctcttctgac cgcaccaatg atcaggttaa ccatactgcg gcgggtggtg
      841 ataaagcaga cgcgtggact gctgggctaa aatacgatgc taacaatatt tacctggcaa
      901 ccatgtattc agaaacgcgt aatatgaccc cgtttggcga cagcgattat gctgttgcaa
      961 acaaaaccca gaactttgaa gtcactgcac agtaccagtt tgattttggt ctgcgtccgg
     1021 cagtctcttt cctgatgtct aaaggccgtg acctgcacgc tgccggtggt gcagacaacc
     1081 cggcaggtgt tgatgataaa gatctggtta aatacgccga tgttggcgcg acttactatt
     1141 tcaataaaaa catgtccact tatgttgact ataaaatcaa cctgttggat gaagatgaca
     1201 gtttctacgc tgccaatggc atctctaccg atgatattgt cgctttaggt ctggtttatc
     1261 agttctaaat cctcctgccc gctgttatgg cgggcttttt ctgcttattc ttcctccttt
     1321 gatctaaatt aaaaatgtga actccgtcat tacacaaaaa gtgtcatctg gcgttacact
     1381 ttatgcggat actaaaacag gaggttttat gaacagaacg attcttgtcc ctatcgatat
     1441 ttccgattca gaattaactc aacgcgtgat tagccacgtt gaggcagagg caaagattga
     1501 tgatgcagag gttcatttcc tgacggtaat accttcactg ccctactatg cctctctggg
     1561 tttagcgtat tccgcagaat taccggcaat ggatgacctg aaagcggaag ccaaatcgca
     1621 actggaagag atcattaaaa aatttaaact gccaaccgac agagtgcatg tccatgttga
     1681 ggaaggctcg cccaaagacc gcattctgga aatggcgaag aagatccccg ctcatatgat
     1741 catcattgct tcccatcgac cggatatcac cacttatctg ctcggttcca acgccgcagc
     1801 tgtagtgcgt cacgcagagt gctccgtgct ggttgtgcgc tgacactaac gcccgcacat
     1861 tgctgcgggc tttttgattc atttcgcaaa tgtgctgaca ttttcccctc taatccgtac
     1921 catacacgcc actgttttta tatcagattt cttatgctgg gcattccggc atgacgcata
     1981 ctcttctgat gccatataac gaattgagtc gcttttaaat gtcgcaaaat caagaaatta
     2041 gtaagaaaga acaatacaac ctgaacaaat tacaaaaacg tctgcgtcgt aacgtgggcg
     2101 aagccattgc tgacttcaat atgattgaag aaggcgatcg catcatggtt tgcctctccg
     2161 ggggtaaaga cagctatacc atgctggaga ttctgcgcaa tttgcagcaa agcgcgccaa
     2221 tcaatttttc gctggtggct gttaacctcg atcaaaagca accgggcttc ccggaacacg
     2281 ttctgcccga gtatcttgaa aagctgggcg ttgagtacaa gattgttgaa gagaatactt
     2341 acggtatcgt gaaagagaag attccagagg gcaaaaccac ttgctcactg tgttctcgcc
     2401 ttcgtcgcgg tatcctttat cgtaccgcaa cggaactggg ggcgacgaag atcgcgttgg
     2461 gtcaccatcg tgacgatatc ctgcaaacgt tgttcttaaa tatgttctac ggcggcaaga
     2521 tgaaaggtat gcctccgaaa ctgatgagcg atgatggcaa acatatcgtt attcgtccgc
     2581 tggcctactg ccgcgagaaa gatattcagc gatttgccga tgcaaaagcg ttcccgatta
     2641 ttccgtgcaa cctgtgcggt tcacagccta acctgcaacg tcaggtgatt gctgacatgt
     2701 tgcgtgactg ggataaacgt tatccagggc gtatcgagac gatgttcagc gcgatgcaga
     2761 atgtggtgcc gtcgcatctg tgcgatacca acctgttcga tttcaaaggc attacccacg
     2821 gttctgaagt ggttaacggg ggtgatctgg cgtttgatcg cgaagagatc ccactacaac
     2881 cggcgggctg gcagccagaa gaagatgaaa atcagttgga tgagttacgg ctgaatgtgg
     2941 ttgaagtgaa ataaccagga tagcgcccga tgcgcaagcg tatcgggcta ctcttatgga
     3001 ggccggataa gacgcggcca gcgtcgcatc cggcaatccc gcataagatg tttactcttg
     3061 cacccggcaa ttcaacattt cattatttta ataaccgcac ccggcacgtt tttcctttaa
     3121 tcttcccgcc cgtaactgtt tccatgcttt atgagcaaca gcctgacgga ccgcgacata
     3181 gacatgcgcc ggatgcacgg cgattttgcc aatatctacg ccatcaagcc cgatgtctcc
     3241 tgtcagcgca cctaatacat cacccgggcg cattttggct tttttcccgc catcgataca
     3301 caacgttgcc atttctgctt ccagcggcaa aatggaacta ttagctggcg gcgtttgcca
     3361 gttaagtttt atctgcaaca tgtcagaaat gatattggcc cgctgtgctt cttccggagc
     3421 acagaaactg atcgccagac cgctatttcc tgcacgagct gtacgaccga tgcgatgtac
     3481 atgaacttca gggtcccacg ccagctcaaa gttcaccacc agctcaagcg atttaatatc
     3541 cagaccacgc gcagcaacat cagtcgcaac cagtacacgg gcgctaccgt tagcaaaacg
     3601 taccagggtc tgatcgcgat cgcgttgctc cagatcgccg tgtaacgaca atgcactttg
     3661 ccctacttca ttcagcgcgt cgcagacagc ctggcaatct tttttggtat tgcaaaacac
     3721 cacacaagag gatggctgat gcaaacttaa taaccgttgc aacagaggaa ttttgccttt
     3781 actggatgtc tcataaaatt gttgttcaat gggtggcaaa gcatctgttg agtcaatttc
     3841 aatcgccaaa ggatcgcgtt gcactcgtcc gctgattgcg gcgatggctt ccggccaggt
     3901 tgccgaaaac agaagcgtct gtcgagatgc aggcgcaaaa cggatgacat catcaatggc
     3961 atcgctaaat cccatatcca gcatgcggtc ggcctcatcc atcaccaaag tattcagcgc
     4021 atccagtgat accgtgcctt tttgcaagtg atccagcaaa cgacccggcg ttgccacgat
     4081 aatatgcggc gcatgttgca acgaatcacg ctgcataccg aacggttgac caccgcacaa
     4141 cgtcaaaatt ttggtatttg gcagaaaacg cgccagccga cgcaattcac ctgccacctg
     4201 atccgccagt tcacgcgtag gacacagcac taaagcctgg gtttgaaata gcgacgcatc
     4261 aatttgctgt aacaagccga ggccaaaagc cgccgttttg ccgctgccgg ttttcgcctg
     4321 cacgcgaaca tcttttccgg caaggatcgc cggaagcgcg gcggcctgca ccggcgtcat
     4381 ggttaaataa cccaactcat taaggttcgt gagttgggcg ggaggcaaaa cattcagggt
     4441 agaaaaagcg gtcacaatct attctcgtgg tcatcgacgc aaagttagca ggcgcgtatc
     4501 ctcgcagatc tacgctcacg atgcgacaat ttaatcggtt cttcatcagg tggtgggtca
     4561 ggcatgggtt gcgggcgagg gatcggatcg ggcactggaa caggatcgcc aggaatcggt
     4621 tcagggacag gaatttgcaa ataaataagt gtcgtcatat ttccctccgg tcattgggtg
     4681 gactcttaaa gggtagacgc tgataaataa caggcaaaaa aaagccgact catcaaagtc
     4741 ggcgtcgtac gaatcaattg tgctatgcag taattcaaaa aaggaagtaa gacaatatgg
     4801 agcgcaacgc ccatcgcttg acgttgcatt cacctgcaag ggagatattg ccctgaatgg
     4861 gtagagagtt tattgacttc gctcaaactt tgcggcgttt ttgtatacag acagccggaa
     4921 aaattgcttt tgttacaacc atttactacg gtgcaaccat aaagcaacac caccaataag
     4981 aacaactaac agaatacaaa aaattgaaaa tccgaattgc cacccgccgc cagggatccc
     5041 accaaggttg acgccaaata accctgtcag aaaggtactg ggtaaaaaga ccattgccat
     5101 caatgacatt gtataggtac gacgagctaa attttcctgc atcacctgag cgatttcatc
     5161 cgccatcacg ccagtccgtg ctatacaggc gtcgatttcg tcaagaccgc gcccaaggcg
     5221 atcggcaata tcctgcatcc gacggcgttg gtcatcgctc atccacggca aacgttcact
     5281 ggcaagacga gcataaacat cacgttgcgg tgccatatag cgacgcatca cgattaattg
     5341 tttgcgcagc agagccagga atccacgcgg tggaatttgc tgatcaagga gattatcttc
     5401 aaggtcgata attttatcgt gcagctgctc gataaattcg ctggaatgat cggtcaacgc
     5461 atcgcacaca tccaccagcc accctccgca atcggtcgga cccgtgccct cttccagatc
     5521 gctcaccaca tcgtccagcg ccagcacttt gcgttgtcgg gtcgaaacaa ttaaccgccc
     5581 gtccatatat acacgcatgg cgaccagttg atcggggcgt tcatcggtgc tgccgtttat
     5641 acagcgcaat gtaataagcg tgccttcacc gagacggctg actcggggcc gtgtgctctc
     5701 gcccgccagc gcatcacgta cgttattggg aagcagcggt gttgtcgcca gccattgggc
     5761 gctatcatgg tgtacataat taaggtggag ccagcaggga tgcgcttcat caatcacatc
     5821 tgtattttcc agcggtttaa cgccgcctct accatccagc atccaggcaa atactgcatc
     5881 cgggacatta acgtccgatc ccttaatcgc ttccacagtg cctccatcat caacgcatta
     5941 ttttgtagtc tagccttctg gccctgttac gcaacatctc atcaccccat taccctgaaa
     6001 tgattaataa aattctgtct aaattgaata caaaaagcaa aatgcttttc cgtatacaaa
     6061 ccgtgtgaag tgttaaatag cgtctatcat tatcagaatt atctgatcat atgacgtggc
     6121 ttttttgcga tcggatagca acaaaaattg ataaaaataa cgggatctca atgattacgc
     6181 acaacttcaa taccctggac ttactcacca gtcctgtctg gattgtttcg ccctttgagg
     6241 aacagttaat ttatgccaat agcgcggcgc gactgttgat gcaagacctc acgtttagtc
     6301 agctacgaac cggaccctat tccgtctcct cacaaaaaga actgccgaaa tacctctccg
     6361 atctgcaaaa ccaacacgat attatcgaaa tcctcactgt tcagcgtaaa gaagaggaaa
     6421 cagcattaag ctgtcggctt gttttgcgag agctgacaga aacagaaccg gtgattattt
     6481 tcgaaggtat cgaagcgccg gcaacgctgg gtttaaaagc cagtcgctcg gcaaattatc
     6541 agcgcaaaaa acaaggtttt tatgcgcgct tttttctgac taactctgca ccaatgttgt
     6601 tgattgaccc ctcacgagat ggccaaatcg tcgatgctaa cctcgccgcg ctcaatttct
     6661 atggttataa ccatgaaacg atgtgccaga aacatacctg ggaaattaat atgctagggc
     6721 gtcgcgtcat gcctatcatg catgaaattt cgcatttacc cggcggtcat aaacctttga
     6781 attttgttca taaactggcg gatggatcta ctcgtcatgt gcagacctat gccgggccga
     6841 ttgaaattta tggcgacaag ctcatgttat gtattgtgca tgatattact gagcaaaaac
     6901 ggctggagga gcagctggaa catgctgctc accatgacgc gatgaccgga ttactgaatc
     6961 ggcgacagtt ttatcacatt accgagccag gccaaatgca gcatctcgcc atcgctcagg
     7021 attacagctt gttgctcatc gacaccgatc gttttaaaca cattaacgat ctctatgggc
     7081 attctaaagg tgatgaggtg ttatgcgccc tcgcccgcac ccttgaaagt tgcgctcgca
     7141 aaggcgattt ggtgtttcgt tggggaggcg aagagtttgt cttattgcta ccaagaaccc
     7201 cactggatac cgcgctttcg ctggctgaaa ctatccgtgt aagcgtggca aaagtgagta
     7261 tttcgggctt accacgcttt accgtcagca ttggtgtggc gcatcacgaa ggaaatgaaa
     7321 gcatcgatga actgtttaaa cgcgttgatg atgctttgta tcgggcgaaa aatgatggac
     7381 gcaaccgcgt gctggcggca taagccgcgg atgcgtctcg agatcaacga ctgcgcttag
     7441 cgtggcgctc ccagttttct tgcttcgcct gcgccgtttt acgtagtgcg acgtaacacg
     7501 ccccgctgcc gccatgatgc ggtagcgcag tgcaatatgc ctgaacatca tcaaattcgg
     7561 tcagccagcg cgccacatag ctgcggacaa tattggcatg cgatttatca tcccgtcctt
     7621 tgccatgaat aatcagcacg ttacgcaaac catcagccat ggcttgttga atgaaactga
     7681 acatcatttt gcggcactct tccaccggct ggcgcaaaag attcaggctc gcctgttgcg
     7741 gatatttacc actgcgcagc ttatccagca ccccatgttg caacccttcc cgccgaaact
     7801 ccagcggctg acttagtggg atgatgtcga gaaatccggt ggtgaggaaa ttatcaagct
     7861 gcagcgtgtc gatacgctgc ggcgcacgtt ggttacgcgt tggatgccag tggacatcgg
     7921 tagcacgttt cagcggctgg acatcttcca tggcgtcaag aaacagcgat ttgtcgtcaa
     7981 ggttcatgtt acatcctccc gcaattaaga gcgcgatatg ataaccagac cgggtcggtc
     8041 caacaacgta ttacccaaaa ttccagtaat aagttccaaa tattgccgat attttaagca
     8101 aaatgct
//
LOCUS       DAZFQD010000189         8044 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.189, whole genome shotgun sequence.
ACCESSION   DAZFQD010000189 DAZFQD010000000
VERSION     DAZFQD010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8044
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.189"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1276
                     /locus_tag="ACHKTW_004709"
     CDS             <1..1276
                     /locus_tag="ACHKTW_004709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO6465597.1"
                     /translation="SYPTGVENHGGSLRIWFHYNGKRVRENLGVPDTAKNRKIAGELR
                     TSVCFAIRMGSFDYAAQFPNSPNLKHFGLGKREITVKALSEKWLDLKKIEIGSNAFSR
                     YQSVVRNMLPRIGEKRLASSVTKEDLLFIRKDLLTGYHNLSNGKTTPIKGRSVVTVNY
                     YMTTIAGMFQFAADNGYIVSNPFNGLTPLKRSRTEPDPLTRDEFIRFIDACHHQQTKN
                     LWSLAVYTGIRHGELIALAWEDIDLKAKTMTIRRNYTKLGEFTLPKTEAGTDRVIHLV
                     QPAVDALKSQAEMTRFDQQYQIDVKLREFGRSTSHECTFVFNPQLVKKCQHVGHHYKA
                     DSIRDSWASALRRAGLRHRKAYQSRHTYACWALSAGANPSFIANQMGHANAQMVFNVY
                     GAWMEDNNIGQIELLNKQLTESVPYMPHRARL"
     gene            1260..2057
                     /gene="yecE"
                     /locus_tag="ACHKTW_004710"
     CDS             1260..2057
                     /gene="yecE"
                     /locus_tag="ACHKTW_004710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000019900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HFO6465598.1"
                     /translation="MEPDSEIISLNISYFNGSCRHINCVEGNTTLYALPKPEVVLRWR
                     EQTTDDFRFCFKFPATISHQAALRHCDDLVTEFLTRMSPLAPRIGQYWLQLPATFGPR
                     ELPALWHFLDSLPGEFNYGVEVRHPQFFAKGEEEQTLNRGLHQRGVNRVILDSRPVHA
                     ACPHSEAIRDAQRKKPKVPVHAVLTATNPLIRFIGSDDMTQNRELFQVWLQKLAQWHQ
                     TTTPYLFLHTPDIAQAPELVHTLWEDLRKTLPEIGAVPAIPQQSSLF"
     gene            2110..2505
                     /gene="yecN"
                     /locus_tag="ACHKTW_004711"
     CDS             2110..2505
                     /gene="yecN"
                     /locus_tag="ACHKTW_004711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HFO6465599.1"
                     /translation="MVSALYTVLSALLLMKFSFDVVRLRLQYRVAYGDGGFSELQSAI
                     RIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            2546..3289
                     /gene="cmoA"
                     /locus_tag="ACHKTW_004712"
     CDS             2546..3289
                     /gene="cmoA"
                     /locus_tag="ACHKTW_004712"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HFO6465600.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLDPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            3286..4257
                     /gene="cmoB"
                     /locus_tag="ACHKTW_004713"
     CDS             3286..4257
                     /gene="cmoB"
                     /locus_tag="ACHKTW_004713"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HFO6465601.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     AVEFLPEIKPYRLDLLHSVTAESEEPLSTGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRVVDVC
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(4422..6851)
                     /gene="torZ"
                     /locus_tag="ACHKTW_004714"
     CDS             complement(4422..6851)
                     /gene="torZ"
                     /locus_tag="ACHKTW_004714"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HFO6465602.1"
                     /translation="MTLTRREFIKHSGIAAGALVVTSAAPLPAWAEEKGGKILTAGRW
                     GAMNVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTARIQHPMVRKSYLDNPLQPA
                     KGRGEDTYVQVSWEQALKLIHEQHDRIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQTSWPLILENSQAVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPIRSETIEFFGDNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLTGKSDNTPKSAAWAAEITGVPEAQ
                     IVKLAELMAANRTMLMAGWGIQRQQYGEQKHWMLVTLAAMLGQIGTPGGGFGFSYHYS
                     NGGNPTRVGGVLPEMSAAIAGQASEAADDGGMTAIPVARIVDALENPGGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDFDVFADLAELLKPGGKEIYTEGKDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEMRRSEKNEQYVRYADFRADPVK
                     NALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAPDEWKGTADEKQLQLLTAHPAHRLH
                     SQLNYAELRKKYAVADREPITIHTEDAARFGIANGDLVRVWNKRGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQLANACAGNSALVYIEKYTGNAPK
                     LTAFDQPAIQA"
     gene            complement(6876..7976)
                     /gene="torY"
                     /locus_tag="ACHKTW_004715"
     CDS             complement(6876..7976)
                     /gene="torY"
                     /locus_tag="ACHKTW_004715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HFO6465603.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFETHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVNPGTD
                     LTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGALTDPALADRKPLWQYAEKLDDTYCAGCHAPIAADHYTVNAWPSIA
                     KGMGARTSMSENELDILTRYFQYNAKDITEKQ"
BASE COUNT         1937 a         2052 c         1913 g         2142 t
ORIGIN      
        1 atcatacccg acaggcgttg aaaaccatgg aggatcactc cgtatatggt ttcactataa
       61 tggcaaacgt gtcagagaaa acctcggtgt tcctgacaca gccaaaaacc ggaagatcgc
      121 tggtgaactt cgcacttccg tttgttttgc aatcagaatg gggagtttcg actacgccgc
      181 gcagttccct aattccccta acctgaaaca ctttggtctg ggaaaaagag agataaccgt
      241 taaggcactt tcggaaaaat ggctggacct taagaaaatt gagataggaa gtaatgcatt
      301 cagtcggtat caatccgtgg tgagaaacat gcttcctcgc ataggggaaa aacgtcttgc
      361 ttcgtcggta acaaaggaag atttactgtt tatcaggaaa gacttgttaa ccgggtatca
      421 taatctctct aacggaaaaa caacgccgat taaaggaagg tcagtagtta cggttaatta
      481 ctacatgacg acaattgcag gaatgtttca atttgcggct gataacggct atatcgtgtc
      541 aaacccattt aacgggctga caccattaaa gagatccaga acagagccag atccgctcac
      601 gcgtgacgaa tttattcgct ttattgatgc ctgtcaccat caacaaacga aaaacctgtg
      661 gtccttagca gtatacacag gcattcgtca cggtgagcta atagctctcg cctgggagga
      721 tattgattta aaagctaaaa caatgactat ccgtcgtaat tatacaaaac tcggggagtt
      781 cactctacca aaaacagagg cgggaactga tcgtgttatt catcttgttc aaccagctgt
      841 tgatgccctg aaaagccagg ctgaaatgac aaggtttgac cagcaatatc aaattgatgt
      901 caaactgcgg gagttcggac gcagcacaag ccatgaatgc acgtttgttt ttaatccgca
      961 actggtgaaa aaatgccagc acgtcggcca ccactataaa gcagattcca tcagagattc
     1021 ctgggcatct gcattaaggc gagcaggact gcggcacaga aaagcctatc agtccaggca
     1081 tacttatgcc tgctgggcat tatcggcagg agcgaatcca agcttcatag caaaccaaat
     1141 gggccatgca aatgcacaaa tggtattcaa cgtttacggt gcatggatgg aagataacaa
     1201 tatcgggcaa atagaactac tcaataagca gttgacagag agtgtcccat acatgcccca
     1261 tagagccaga ctctgaaata atatctttaa atatcagtta ttttaatggt agttgccgtc
     1321 acattaactg cgtggagggc aacaccacgc tttacgccct gccgaaaccc gaggttgtcc
     1381 tgcgctggcg tgagcagacc acagatgact tccgcttctg ttttaagttt ccggcgacca
     1441 tttcgcatca ggcagcatta cggcattgcg atgatttagt gactgaattt ttgacccgca
     1501 tgtcaccgtt ggctccgcgc attgggcaat actggctgca actgcctgcc acattcggcc
     1561 cacgggagct gcctgcgctt tggcattttc tcgattctct ccctggcgaa tttaattatg
     1621 gtgtggaagt ccgccatcca cagtttttcg ccaaagggga agaggaacaa acgcttaatc
     1681 gcggtttaca tcagcgcggc gttaatcggg tgattttaga cagccgcccg gttcatgcag
     1741 catgtccaca cagtgaagct attcgcgacg ctcaacgaaa aaaacctaaa gttccggtac
     1801 atgctgtact gacggcgaca aatccgctga ttcgttttat cggtagtgat gatatgacgc
     1861 aaaaccggga attatttcag gtctggttac agaaattagc gcagtggcat cagaccacta
     1921 cgccttatct ttttttacat acgccagata ttgcccaggc cccggaactg gtacataccc
     1981 tgtgggaaga cttacgtaaa acgcttccag agatcggagc agttccggct attccacagc
     2041 aatcttctct tttctgaatt tgccacctat catagacagg tgcaatcggc cattttaaag
     2101 ggagtttgta tggtaagcgc gctgtatacc gttttaagtg cgttgttatt aatgaagttc
     2161 tcttttgatg tcgttcgctt gcgtttgcag taccgcgttg cctatggcga cggcggtttt
     2221 agcgaactgc aaagcgctat tcgcattcat ggtaacgcgg tggaatatat tcccatcgcg
     2281 attgtgttga tgctgtttat ggaaatgaat ggcgcagaaa cctggatggt gcatatttgc
     2341 ggcatcgttt tgcttgctgg tcgtctgatg cattattacg gttttcatca ccgtctgttc
     2401 cgctggcgac gttccggcat gagcgccacc tggtgtgcgc tgttgctgat ggtgctggcg
     2461 aatctttggt atatgccctg ggagttggtt ttctccctgc gttagcgcac aatacgccac
     2521 tttctttttc ccggattttt acgttatgtc tcaccgcgac acgctatttt ctgcccctat
     2581 cgccagactg ggcgactgga cctttgatga acgggtagct gaagtcttcc cggatatgat
     2641 ccagcgttcc gttcccggct attccaatat tatttccatg attggtatgt tagccgagcg
     2701 cttcgttcaa cctggtacgc aggtttacga tctgggttgt tctctgggcg cggcgacgct
     2761 ctcggtgcgt cgcaacattc atcatgataa ttgcaaaatt attgccatcg acaactcccc
     2821 ggcgatgatt gaacgctgcc gtcgtcatat tgacgcctat aaagccccta cgccagtaga
     2881 cgtcattgaa ggtgatattc gcgatatcgc cattgaaaac gcatcgatgg tggtgctgaa
     2941 ttttaccctg caattcctgg atccttccga gcgccaggca ttactggata aaatttatca
     3001 aggactgaac ccaggcggcg cgctggtgct ttcggaaaaa ttcagtttcg aagatgccaa
     3061 agttggtgaa ctgttgttca acatgcacca cgactttaaa cgtgccaacg gttacagcga
     3121 actggagatc agccagaagc gcagcatgct ggaaaacgtg atgctgaccg attccgtgga
     3181 aacccataaa gcacgcctgc ataaagccgg ttttgagcat agcgagctgt ggttccagtg
     3241 ctttaacttt ggttcactgg tggcattaaa agcagaggac gctgcatgat cgactttggt
     3301 aacttttatt ctctgattgc caaaaatcat ctttcacact ggctcgaaac gctgcccgcg
     3361 cagattgcta actggcagcg cgagcagcag cacgggctgt ttaagcagtg gtccaacgcg
     3421 gtggaatttc tgcctgaaat taaaccgtat cgtctggatt tattgcatag cgtaaccgcc
     3481 gaaagcgaag agccactgag caccgggcaa attaaacgca ttgaaacgct tatgcggaac
     3541 ctgatgccat ggcgcaaagg gccgttctca ctgtatggcg tcaacatcga taccgaatgg
     3601 cgttccgact ggaaatggga tcgtgttctg ccccatcttt ctgatttaac cggacgcacc
     3661 attctggatg tcggctgcgg cagcggttat cacatgtggc gcatgattgg cgcaggcgcg
     3721 cacctcgcgg tggggatcga ccctacgcaa ctgtttctgt gccagtttga agcggtgcgt
     3781 aaactactgg gtaacgatca gcgcgcacat ttgttaccgt taggtattga acagcttccg
     3841 gcactgaaag cctttgatac cgtcttttcg atgggcgtgc tttaccatcg ccgttcgccg
     3901 ctggagcatc tctggcagtt gaaagatcaa ctggtgaatg agggtgaact ggtgctggaa
     3961 acgctggtta ttgatggcga tgagaacacg gtgctggtac caggcgatcg ttacgcgcaa
     4021 atgcgtaatg tctatttcat cccttccgcg ctggcgctga aaaactggct gaagaagtgt
     4081 ggttttgtag atatccgcgt tgtggatgtg tgcgttacca ctactgaaga gcagcgacgc
     4141 actgaatgga tggttaccga atctctgtcc gatttcctcg acccacatga tccgagtaag
     4201 acagttgaag gttatcctgc gcctaaacgc gcggtgctga ttgcgcgcaa gccgtaaagg
     4261 tctggcatta ctgccggatg cggcttgaat gccttatccg gcctacaaac ccttgctaat
     4321 tcaatctatt gcagggacta tgtaggcctg ataagcatag cgcatcaggc agttttacgt
     4381 ttgcataacc tcagcgcccg tttccgggcg ctattcactt cttatgcctg aatagctggc
     4441 tgatcaaacg ccgttaactt cggcgcattg cccgtatatt tttcgatata caccagcgca
     4501 gagttaccgg cacaggcatt tgccagctgc gagctgggga tatccgccgt taacacgttc
     4561 gcactgccgt ttttacacaa gccattttcc agatctggcc atgcaccttc atgcacgcat
     4621 accacgcctt ttttgatccc gtcagtcacc accgcgcctg tcagaatctg accacgtttg
     4681 ttccacacac gcaccagatc accattcgca ataccaaagc gagcagcatc ttcggtgtga
     4741 atagtgattg gttcacgatc tgcaaccgca tattttttac gcagttccgc atagttaagc
     4801 tgactatgta aacggtgtgc cggatgtgcg gtcagaagct gcaactgctt ctcgtcggcg
     4861 gtacccttcc actcatcagg cgcaagccag gttgggtgtg ccgggcaatc cttatagcca
     4921 aatttttcca gcgttttgga gtaaatctca attttgccgc ttggcgtacc cagcgcattt
     4981 ttcaccggat cggcgcggaa atcagcataa cgaacgtact gttcgttctt ctcgctgcgg
     5041 cgcatttcga tcagtttatt ttgctgccag aaggcattaa acatcggcat agtcacgcgt
     5101 tgcgcacgag cacctttctg agcggcatca tagaaaaatt tcagccacgc catttcatct
     5161 ttaccttcgg tatagatctc ttttccgcca ggtttcagta attcagcaag atcggcaaat
     5221 acgtcaaaat cgttacgcgc ttcaaattgc ggagcaacag cctgcttcat cggcacaata
     5281 tgctggttgc tgtaatcacc ggtcatcgtc aagtcattgc gctcaaacga tgtggtgatc
     5341 ggtaatacga tatctgcatg tttagcagct gcggtccagt agcattcaga aacgacgatc
     5401 atctccggtt tctgccatgc tttgatcaga cgattagtat cctggtgatg ggtaaagtta
     5461 ccgccacccg cccaccagat cattttgata ttcggatacg tttgttcttt accgttatgc
     5521 tgatatttgc caccaggatt ttccagcgca tcgacgatac gggcaacagg aatcgccgtc
     5581 attccgccat catcagcggc ctcgctggcc tgtccggcaa tcgccgcaga catttccggc
     5641 agcacgccgc caacacgcgt cggattgcca ccgttggagt aatgataaga gaaaccaaaa
     5701 ccgccgcccg gtgtaccaat ctgccctaac atcgccgcca gcgtcaccag catccagtgt
     5761 ttctgttcac catattgctg gcgctgaatt ccccagccag ccatcagcat tgtgcgattc
     5821 gccgccatca gttcagcgag tttgactatc tgcgcttccg gtacgccagt aatttccgcc
     5881 gcccaggccg cgctcttcgg agtattatcg ctcttacctg tcagatactc ttcaaactgc
     5941 ggatacccgg tggtgtattt ttcaaggaac actttatcgt gtttaccttg tgtcatcagg
     6001 gtatgcgcaa tccctaacat cagtgccacg tcggtgccca tattcggcgc gatccaggta
     6061 gcattatcac caaagaactc gatggtttcg gagcggatag gatcaatggc aatcactggt
     6121 ttgccagatt ttttcagctg atggaagtat tccagccctt gctcatcggt actgctccag
     6181 gcaattttta aggtattcag cgggttcatc ccccacagca ccacagcctg gctgttttcc
     6241 agaatcagcg gccaggaggt ctgctgttca tacacctcta cagaaccgac cacatgcggc
     6301 atgatcacct gtgctgcccc ggttgaataa tcgccgctat gcccggaata accgcccgcc
     6361 aggttcatat aacgttgcag taaggtttgc gctttatgca acacgccaga agagcgccag
     6421 ccgtatgatc cggcaaaaat agcagatggt ccgttagctt tgcgaatgcg atcgtgttgc
     6481 tcatgaatca gttttaatgc ttgctcccag ctcacctgta cataagtatc ttcgccacga
     6541 cctttcgccg gttgcagtgg attatcgaga tagctttttc ttaccatcgg atgctgaata
     6601 cgtgccgtgg tgtgtacctg atccgccgcc gtagactgta aggagttcgg tatggttttc
     6661 gccagcgcgc ctgttgaaga aacaatcttg ccgtccttca cttctacgtt catcgctccc
     6721 caacgtcccg cggtgaggat tttaccgccc ttctcttctg cccatgcggg gagcggtgct
     6781 gccgatgtca ccaccagcgc tccagcggca ataccgctgt gtttaataaa ttcacgtctt
     6841 gttaatgtca taacttcctc cctgatcaac gaggatcact gtttctcggt aatatctttg
     6901 gcgttgtact ggaaataccg cgttaaaatg tccagttcgt tttcgctcat gctggttcgc
     6961 gcacccattc ctttggcaat ggacggccat gcattgacgg tgtaatggtc agcggcaata
     7021 ggcgcatggc aaccagcgca ataggtatcg tcaagttttt cagcgtattg ccatagcggt
     7081 ttacggtccg ctaatgcggg atcggtaagc gcaccctgta aagacgcctg acgccattga
     7141 ttgccgtatt cgtcagcctg ccattccccg tttaccgtga gcgccttgat accttcttca
     7201 cttaatgtgg ctagcgccag ccgttgacct gccgccaggt agagcgtgtt ttcactgccc
     7261 tgcatttgat aaccctgcaa cagaacgatc ggctgtttgc cactggcatc aacgacggtg
     7321 agatcggtac caggattcac ggtagccagc tcgcctatgc gagaagtttt gaaaggataa
     7381 atatgtgcgc cattagtcac tgaagtagcg gcctgacttt ccagctcatg cgccgcgttg
     7441 tcatccattt ttatttctgg cggaaaatgg gcaatgcctt tatgacaatc gatacaggtt
     7501 tcgccgccct tttgtgcttt gttatgcatt ttctgcgcag attcactttg cgaggcaata
     7561 tccatggcat caaaagaatg gcaactacgg cacgttgcag agtcagttgc ttttaattct
     7621 ttccatactg tttcggccat ttcctggcga tgagtttcga acttatcgtc actgtctatt
     7681 ttgccgctaa caaattcatg ataaatatct ttagatgctt ttaatttagc aaataaataa
     7741 tccatccctg actttggaat atggcaatct gcacattccg cacgtatccc tttctggttc
     7801 gaaaagtgga cagttccctg atattcctca aaaggtttac tcatcgagtg gcaggaaagg
     7861 caaaatgctg tatccgacgt tttatgtaag gctttttgcg ccagcaataa cccgccgcca
     7921 ccaaccacaa ccgctatcag caaaaacaat aacccaatgc gttttttccc tcgcatagat
     7981 tcctctaaat aaattccaga acaccgaaat cattgtataa ttaaatacac aacgaattaa
     8041 ccgc
//
LOCUS       DAZFQD010000190         7953 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.190, whole genome shotgun sequence.
ACCESSION   DAZFQD010000190 DAZFQD010000000
VERSION     DAZFQD010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7953
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.190"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..380
                     /gene="vasI"
                     /locus_tag="ACHKTW_004716"
     CDS             <1..380
                     /gene="vasI"
                     /locus_tag="ACHKTW_004716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     VasI"
                     /protein_id="HFO6465604.1"
                     /translation="RPTVVITTPAIGHVPPRPVLMFSCVDNITRMQVALMHPLDVHDI
                     AVTLNADNRALRSHWFVRENGTLLESSRGLSGIDEIKQLFGAKTLTVDTGADNAAGKL
                     TFNIDGLARAIAPLRDACHWAGE"
     gene            385..1797
                     /gene="tssA"
                     /locus_tag="ACHKTW_004717"
     CDS             385..1797
                     /gene="tssA"
                     /locus_tag="ACHKTW_004717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HFO6465605.1"
                     /translation="MAMDLRDPNVWISHLLENLPEEKLASALKDDNPNWEYIDGEIVK
                     LGSLAHSQLDIPELQRRGLVILASESKDFRLLAHLLRTLQHAGDPHLALRLLALYVEH
                     YWAVAAPQNMAHKKRFASQVIKRFETGIEVFSQNADTAQRDALSGELAKLAQCWQSHN
                     APELAQATDDLFALYQRAFNRAAPAPVPTPAASGSSPQTTATSESGVTQPSAPAPQIV
                     IDSHDDKAWRDTLLKVAAILCERQPDSPQGYRLRRHALWQNITSTPQAESDGRTPLAA
                     VSADMVADYHAQLGSADMALWQQVEKSVLLAPYWLDGHCLSAQTALRLGYKQVADAIR
                     DEVIRFLERLPQLTGLLFNDHTPFISEQTKQWLAASPDAKVAPVAQIGEESKAARACF
                     AEQGLEAALRYLDMLPEGDPRDQFHRQYLAAQLTEEAGLVQLAQQQYRMLFRMGLQMM
                     VADWEPSLLEQLEQKFTAEQ"
     gene            1905..5339
                     /gene="tssM"
                     /locus_tag="ACHKTW_004718"
     CDS             1905..5339
                     /gene="tssM"
                     /locus_tag="ACHKTW_004718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system membrane subunit TssM"
                     /protein_id="HFO6465606.1"
                     /translation="MLILAWIFLLVWIWWKGPTWTLYEEQWLKPLANRWLATAAWGII
                     ALMWLTVRVMKRLQQLEKMQKQQREEAVDPLSVELNAQQRYLDRWLLRLQRHLDNRRF
                     LWQLPWYMVIGPAGSGKTTLLREGFPSDIIYAPEGARGAEQRLYLTPHVGKQAVIFDI
                     DGTLCAPADADILHRRLWEHALGWLKEKRARQPLNGIILTLDLPDLLTADKRRREHLL
                     QALRSRLQDIRQHLHCQLPVYVVLTRLDLLQGFAALFQSLNRQDRDAILGVTFTRRAH
                     ENDDWRTELNAFWQTWVDRMNLALPDLMVAQTHTRASLFSFSRQMQGSREPLVSLLEG
                     LLDGENMNVMLRGVYLTSSLQRGQMDDIFTQSAARQYRLGNNPLASWPLVDTAPYFTR
                     SLFPQALLAEPNLATESRAWLIRSRRRLTVFSATGGVAALLLITGWHHYYNGNYQSGI
                     TVLKQAKAFMDVPPPQGEDDFGNLQLPLLNPVRDATLAYGDWGDRSRLADMGLYQGRR
                     IGPYVEQTYLQLLEQRYLPSLFNGLVKELNAAPPESEEKLAVLRVMRMLEDKSGRNNQ
                     VVKQYMAKRWSEKFHGQRDIQAQLMSHLDYALAHTDWHAERQAGDGDAISRWTPYDKP
                     VVSAQKELSKLPVYQRVYQSLKTRALGVLPADLNLRDQVGPTFDQVFTSADDNKLVVP
                     QFLTRYGLQSYFVKQRDELVELTAMDSWVLNLTRSVKYSDADRAEIQRQLTEQYISDY
                     TATWRAGMDNLNIRNFESIGQLTGALEQVISGDQPLQRALTVLRDNTQPGVFSEKLSA
                     KEREEALAEPDYQLLTRLGHEFAPENSTLAVQKDKESTMQAVYQQLTELHRYLLAIQN
                     APVPGKSALKAVQLRLDQNSSDPIFATRQMAKTLPAPLNRWVGRLADQAWHVVMVEAV
                     HYMEVDWRDSVVKPFNEQLANNYPFNPRSAQDASLDAFERFFKPDGILDTFYQQNLKL
                     FIDNDLSLEDGDNNVIIREDIIAQLETAQKIRDIFFSKQNGLGTSFAVETVSLSGNKR
                     RSVLNLDGQLVDYSQGRNYTAHLVWPNNMREGNESKLTLIGTSGNAPRSISFSGPWAQ
                     FRLFGAGQLTGVQDGNFTVRFSVDGGAMTYRVHTDTEDNPFSGGLFSQFGLSDTLY"
     gene            5350..6799
                     /locus_tag="ACHKTW_004719"
                     /pseudo
     CDS             5350..6799
                     /locus_tag="ACHKTW_004719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308244.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VasL domain-containing protein"
     gene            6726..7208
                     /locus_tag="ACHKTW_004720"
     CDS             6726..7208
                     /locus_tag="ACHKTW_004720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HFO6465607.1"
                     /translation="MANISYLSLSGETQGLISAGCSTLDSVGNKAQPEHKDQIMVYAL
                     MHSISRSQNVNHHELIITKPVDKSSPLLAKALSDNEKMAICEFILYRTSKAGIYQPYY
                     KINLSKARISSIDFVTPHAVLEKELEPQERIAFIYEDISWEHTLAGTNAMSKWQDRVQ
                     "
     gene            7252..>7953
                     /locus_tag="ACHKTW_004721"
     CDS             7252..>7953
                     /locus_tag="ACHKTW_004721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6465608.1"
                     /translation="MAGLRNNNNTQNAQWADYVGDILRGAQPINQLVPQHPYLNDVPL
                     IDELRHQNTHHVILLTLDVAKKILSPITSFDYIHFITTHPSGIKDTLAWLVNASKLMT
                     EFDDNGKIIFNLNALKYTKASYFEILGEKYIKITTSSPWLLEKLGKYIFSSRAPQVLE
                     LAIGWRGALSESIKGVKFCIWFSVAWRTIEFIMSSERDLVNFLGDFSMDVAKAVIAGG
                     VATAIGSLTSFACVSF"
BASE COUNT         1957 a         2113 c         2175 g         1708 t
ORIGIN      
        1 agcgcccgac ggtggtgata accaccccgg ctatcggtca tgttccgcca cgcccggtgc
       61 tgatgttcag ttgcgtggac aatatcaccc ggatgcaggt ggcgctgatg caccctctgg
      121 atgtccatga tatcgccgtc actctgaatg ccgacaaccg ggcattgcgc agccactggt
      181 ttgtgcgcga gaacggcacc ctgctggagt ccagccgggg gttatccggg attgatgaaa
      241 ttaagcaact gttcggcgca aaaacgctga cggtggatac cggcgcagac aatgccgccg
      301 gaaagctgac ctttaacatt gacgggctgg caagggcgat tgctcccctg cgcgatgcct
      361 gccactgggc gggagaataa aaatatggca atggacttac gcgatccgaa tgtctggata
      421 tcgcacctgc tggaaaacct gccggaagag aaactggcat cggcgctgaa agatgacaac
      481 ccgaactggg agtatatcga cggcgaaatc gtcaagctgg gatcgctggc ccacagtcaa
      541 ctggatatcc cggaactcca gcgccggggg ctggtcattc tggcttcgga gagtaaggat
      601 tttcgtctgc tggcgcatct gctgagaacc ctgcaacacg ccggtgatcc tcatctcgca
      661 ttgcgcctgc tggcgctgta tgtggagcat tactgggcgg tggctgcgcc gcagaatatg
      721 gcgcataaaa aacgctttgc cagccaggtc attaaacgct ttgaaacagg tattgaagtt
      781 ttttcacaga acgccgacac agcgcagcgc gatgcgctgt caggggaact ggcaaaactg
      841 gcgcaatgct ggcagtcaca taacgccccg gaactggcac aggctactga tgatcttttt
      901 gccctgtacc agcgtgcgtt taatcgtgcg gcaccggctc ctgtccccac tccggcggcc
      961 tccggtagtt caccacagac caccgccacg tctgaaagcg gcgtgacgca acccagtgct
     1021 ccggctcccc aaatcgtcat cgacagtcac gacgacaaag cctggcgcga cacgctgtta
     1081 aaagtggcgg ccattttatg tgaacgccag ccggattcac cgcagggcta ccgtctgcgc
     1141 cgccatgcgc tttggcagaa catcaccagt acgccccagg cggaaagcga tggccgtact
     1201 ccactggctg cggtctccgc cgatatggtg gcagattacc atgctcagct cggtagtgcc
     1261 gatatggcgc tgtggcagca ggtggagaaa agcgtactgc tggctcctta ctggctggat
     1321 ggtcactgcc tttctgcaca gacggcgctg cgtctgggtt acaaacaggt ggcagacgcc
     1381 atccgcgatg aggtcatccg cttccttgag cgtctgcccc agcttaccgg gctgctgttt
     1441 aatgaccaca caccgtttat cagtgagcag acgaaacaat ggctggcagc ttcgcccgac
     1501 gccaaagttg caccggttgc gcaaatcggt gaggaatcga aggcagccag agcctgtttt
     1561 gctgagcagg gtctggaggc ggcgctgcga tatctggaca tgctacccga aggcgatccc
     1621 cgcgatcagt ttcaccgcca gtaccttgcc gcacagttga cggaagaggc ggggctggta
     1681 cagcttgcgc agcagcagta ccggatgttg ttcaggatgg gattgcagat gatggtggca
     1741 gactgggagc cgtccctgct tgagcagcta gaacaaaaat tcacggcaga acaataaaag
     1801 agaacaggag tttttgtgtt cagattacca acaccccgac tactcagcgg acaaaatcag
     1861 ccctgcgacc ggcgatgccc cggtttaaag tctctgcttt ctggctgctg atactggcgt
     1921 ggatttttct gctggtgtgg atctggtgga aaggcccgac gtggacgctg tatgaagaac
     1981 agtggctcaa accactggcg aaccgctggc tggcaacggc ggcgtggggg attattgccc
     2041 tgatgtggct taccgtccgg gtgatgaagc gcctgcaaca gcttgaaaaa atgcaaaagc
     2101 aacagcgcga ggaagccgtt gatccgctca gcgtggaact gaacgcccag cagcgttatc
     2161 ttgaccgctg gctgctgcgc ctgcaacgcc atctcgacaa ccgccgtttc ctgtggcagt
     2221 tgccgtggta tatggtcatc ggcccggcgg gcagtggcaa aacgacactg ctgcgcgagg
     2281 ggtttccgtc cgacattatt tatgccccgg agggcgcacg cggcgcagaa caacgcctgt
     2341 acctcacgcc ccatgtcggt aaacaggcgg tgatctttga tatcgacggc acactctgcg
     2401 ctcccgctga tgcggatatc ctgcatcgcc gtctgtggga acatgccctc ggctggctga
     2461 aagaaaagcg cgcgcgccag ccgctgaatg ggattattct gacactcgat ttacccgatc
     2521 tgcttaccgc agacaaacgc cgccgcgagc atctgttaca ggcgctgcgc agccgcttgc
     2581 aggatatacg ccagcatctt cactgccagt taccggttta cgtggtactt acccggcttg
     2641 atttattgca gggttttgcc gccctgtttc agtccctgaa cagacaggat cgcgatgcga
     2701 ttctgggcgt cacgttcacc cgccgtgccc atgaaaatga tgactggcga acagagttga
     2761 atgctttctg gcagacatgg gtggatcgaa tgaatctggc gttgccggat ctgatggtcg
     2821 ctcagactca cacccgcgcg tctttattca gtttttcccg ccagatgcag ggaagccgtg
     2881 aaccgctggt gtcactgctt gagggtctgc ttgatggcga aaatatgaac gtgatgctgc
     2941 gtggtgtcta tctcacctct tcgcttcagc gtggacagat ggatgatata ttcacccagt
     3001 ctgccgcccg ccagtaccgg ctgggcaata acccactggc gtcctggccc ctggtggaca
     3061 ccgcgcctta tttcacccgc agcctgttcc cgcaggcatt actcgcagag cctaatctgg
     3121 caacagagag ccgcgcctgg ctgatacgtt cccgtcgccg cctgacggtt ttctctgcca
     3181 caggcggcgt ggcagcactg ctgctcatca ccggctggca tcactattac aacggtaact
     3241 atcagtctgg catcaccgtg cttaagcagg ccaaagcctt tatggacgtg ccgcctccgc
     3301 agggggaaga tgactttggc aacctgcaac tgccgctcct gaacccggta cgcgatgcca
     3361 cactggccta tggcgactgg ggcgaccgca gccgtctggc cgatatggga ctgtaccagg
     3421 gacgacgtat cgggccttat gtggaacaga cctatctgca actgctggag caacgttacc
     3481 tgccctcgct gtttaacggg ctggtcaaag agctgaacgc cgcgccgccg gagagtgaag
     3541 aaaaactcgc ggtgctgcgc gtgatgcgaa tgctggagga caaaagcgga cgtaacaatc
     3601 aggtggtgaa gcagtatatg gcaaaacgct ggagcgaaaa gttccacggt cagcgcgata
     3661 tccaggcaca actgatgtcc catcttgact acgcgctggc tcatactgac tggcacgcag
     3721 agcgtcaggc gggcgacggt gacgccatca gccgctggac gccatatgac aagcccgtgg
     3781 tatcagcaca gaaagaactg agcaaactgc ctgtctacca gcgggtttac cagagcctga
     3841 aaacgcgggc gctgggcgtt cttcctgccg acctcaatct gcgtgaccag gtagggccaa
     3901 cctttgacca ggtgtttaca tctgccgatg acaacaaact ggttgttcca cagtttctta
     3961 cccgttacgg cctgcaaagc tattttgtaa aacagcgcga tgaactggtt gaactgacgg
     4021 cgatggattc ctgggtactt aaccttaccc gcagcgtgaa atacagtgac gccgaccgcg
     4081 cggaaatcca gcgccagttg accgagcagt atatcagcga ctacaccgcc acctggcggg
     4141 ccgggatgga caatctgaat atccgcaatt ttgagtccat cggacaactg accggggcgc
     4201 tggagcaggt tatcagcggc gaccagcctt tgcagcgggc gctaaccgtg ctgcgtgaca
     4261 acacacagcc aggcgtcttt tctgaaaaac tctctgccaa agaacgggag gaagccctgg
     4321 cagagccgga ttaccagtta ctcacccgcc tcgggcatga attcgccccg gaaaacagta
     4381 ccctggcagt acagaaagac aaagaaagca cgatgcaggc cgtgtatcag caactcaccg
     4441 agttgcaccg ctacctgctg gcaatccaga acgcgcctgt accaggaaaa tcggcgctga
     4501 aagccgtgca gttacggctt gatcagaaca gcagcgatcc gatattcgcc acccgccaga
     4561 tggcaaaaac gctgcctgct ccgctcaacc gctgggttgg caggctggct gaccaggcct
     4621 ggcatgtggt gatggtggag gctgttcatt atatggaagt ggactggcgc gacagcgtgg
     4681 tgaaaccgtt taacgagcaa ctggcaaata actatccgtt taatccgcgt tctgcacagg
     4741 atgcctcact ggatgccttc gaacgcttct ttaaaccgga tggcatactg gataccttct
     4801 accagcagaa cctgaagctg tttatcgata atgacctgag tctggaggat ggcgataaca
     4861 acgtcattat tcgcgaagat attattgcgc aactggaaac tgcgcagaaa atccgtgaca
     4921 tcttcttcag caaacagaac ggtctgggaa catcctttgc cgtggaaacg gtatcgcttt
     4981 caggcaataa acgccgcagt gtactgaacc ttgacggtca gttagtcgat tacagccagg
     5041 gccgtaacta taccgcccat ctggtctggc ctaacaacat gcgcgaaggc aacgaaagta
     5101 agctgacgct catcggcacc agcggcaacg cgccgcgcag tatcagcttc agcgggccgt
     5161 gggcgcagtt ccgcctgttc ggggccggac aactgaccgg agtacaggat ggcaacttta
     5221 ccgtgcgctt tagcgtggac ggtggcgcga tgacctaccg tgtgcatacc gacacggaag
     5281 ataacccgtt cagcggtggg ttgttcagcc agtttggtct gtcagacaca ctgtactgag
     5341 gaggcaggga tgaatagtaa cgtactgaca caaactatcg ttaccggcag tgacccgcgc
     5401 gggctgccgg aattcagcgc catccgcgag gaaataaaca aagccagcca cccgtcacag
     5461 cctgagctga actggaaact ggtggagtcg ctggcgctgg cgatttttaa agccaacggt
     5521 gtggatttac acaccgccac ctactatacg cttgcccgga cacggaccca gggactggcg
     5581 ggattctgcg aaggtgcgga actgctggcg gcaatggtaa gccacgactg ggataagttc
     5641 tggccgcagg gcggcccggc gcgtactgaa atgctggact ggtttaactc ccgaaccggc
     5701 aatattctgc gtcagcaaat ctcctttgcg gaatccgacc taccactgat ctaccgcaca
     5761 gagcgggcat tgcagcttat ctgcgataag ctccagcagg tggaactgaa gcgcgttccg
     5821 cgcgtggaga atctgctcta ttttatgcag aacacgcgta aacggcttga accccagctg
     5881 aagagtaaca ctgagaacgc cgcacagacc acggtcagaa cgctgattta tgtcccggaa
     5941 acacaggcat cttccacacc agaagcggta gtgcctcccc tgcccggcct acctgagatg
     6001 aaagtggaag tgcgcagtct gacagagaat cccccacagg ccagtgtgat aaagcaaggc
     6061 agtacggtaa gagggtttat cgcagggatc gcctgttcag tggctgtcgc ctcagcattg
     6121 tggtggtggc aggtctatcc ggtgcagcag caactgttac aggttaacga caccgctcag
     6181 ggcgcagcaa cggtgtggat ggcctcacct gaactcgaaa actatgagcg caggctgcaa
     6241 caacttcttg atacctcccc ggtacagccg ctggaaaccg ggatgcagat gatgcgtgtt
     6301 gccgacagtc gctggccgga aagcctgcaa cagcaacagg cctcgacaca atggaatgag
     6361 gcactcaaaa cccgcgcaca gagtagcccg cagttgcgtg gctggttgca gacccgccag
     6421 gacttacatg cttttgcaga tctagtgatg cagcgcgaga aagagggact aaccctttcc
     6481 tatatcaaaa atatcatctg gcaggcggag cggggactgg ggcaggaaac acccgttgag
     6541 tctctgttga cgcagtacca tgatgcccgt gcgcagaagc agaatacaga tgcgctggaa
     6601 aaacaaatta atgagcgact cgaaggcgtg ttaagccgct ggctgctgct gaagaataac
     6661 gtcatgccag aggcggcaac cggtaccacg gccgaaaaat aattatttaa ttaaaggaga
     6721 ttatcatggc aaatataagt tatttatctt tgagtggaga gacacaaggg ctaatttcag
     6781 caggttgctc aacgcttgat tctgtcggaa acaaagcgca gccagaacat aaagatcaga
     6841 tcatggttta tgcgttaatg cattccatct caagaagtca aaatgtcaat catcatgaat
     6901 taattatcac caaaccggtt gataaatcct caccattgct ggcaaaagca cttagtgaca
     6961 atgaaaaaat ggcaatatgt gaatttatac tctaccgaac cagtaaagcg ggtatttacc
     7021 agccttatta taaaataaat ctgtcaaaag cccgaatatc aagtattgat tttgtcacac
     7081 cgcatgctgt cctggaaaaa gaactggaac cacaggagag aattgccttt atttatgaag
     7141 atatttcctg ggagcatacg ttagccggta cgaatgcaat gagtaaatgg caggatcgtg
     7201 tacagtaact tactgtaata acataataaa ttactattgc ggagagataa aatggctggt
     7261 ttaagaaata acaacaacac acagaatgca cagtgggctg attatgtggg tgacattctt
     7321 cggggggctc agccgataaa ccagctagta cctcagcacc cttatcttaa cgacgtccca
     7381 ctcattgatg agttaaggca tcagaacact catcatgtta ttctcttgac acttgatgtt
     7441 gcaaaaaaaa tactcagccc aataacatca tttgattaca ttcattttat cactacacat
     7501 ccttcaggta taaaagatac actcgcctgg ttagttaacg cgagtaaatt aatgactgag
     7561 tttgatgaca atgggaaaat tatatttaac cttaatgcct taaagtatac aaaagcaagt
     7621 tactttgaaa tacttggaga gaaatatata aaaatcacaa catccagccc ctggctttta
     7681 gaaaagctcg gtaaatatat tttctcttca cgagcgcctc aagttctgga actcgctatt
     7741 ggctggcgag gagctttatc tgaatcaata aaaggagtta agttctgtat ctggttttca
     7801 gtagcctgga gaaccataga atttattatg agttcagagc gagatctggt taattttctt
     7861 ggcgattttt caatggatgt ggccaaagca gttatagccg gtggtgttgc aaccgctatt
     7921 ggaagcctga cttcttttgc ctgtgttagc ttt
//
LOCUS       DAZFQD010000191         7946 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.191, whole genome shotgun sequence.
ACCESSION   DAZFQD010000191 DAZFQD010000000
VERSION     DAZFQD010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7946
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.191"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            4..642
                     /locus_tag="ACHKTW_004722"
     CDS             4..642
                     /locus_tag="ACHKTW_004722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6465609.1"
                     /translation="MWSRLSEQLTRCALCVCDPKHKHGDDSRYQAGGQCNQSGSVRCH
                     TCNERFSLYSLRNCSRAKAHGANLSDSCSIFLRRLFRAGDVFSVDSSLVIVCACKAMR
                     RSTSHHGADSFYPLGSMPRCCNSLRIIRFIQAARESSPSCCCAFSIFSRKSGSKRNWN
                     GGLPRLSFLCVDTLITPHVMYLCVMTHYTQSTQKAKPRTVGAVTGLLTNNVN"
     gene            651..989
                     /locus_tag="ACHKTW_004723"
                     /pseudo
     CDS             651..989
                     /locus_tag="ACHKTW_004723"
                     /inference="COORDINATES: protein motif:HMM:NF022029.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
     gene            1020..1208
                     /locus_tag="ACHKTW_004724"
     CDS             1020..1208
                     /locus_tag="ACHKTW_004724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HFO6465610.1"
                     /translation="MATIPTLVHSQTAFIWRFIIFGASESQIIHVTAWTEREARSRCP
                     SGCVAVFAARIRQGSHHA"
     gene            1201..1665
                     /locus_tag="ACHKTW_004725"
     CDS             1201..1665
                     /locus_tag="ACHKTW_004725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001230911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HFO6465611.1"
                     /translation="MRKNRLQKIITGLYASPVAHMATGFSSLNTTADSRLHSDMRFFC
                     VRKPTHTRIMAGRNGGAFALAGSCSASLLTLLRLATPFSSVVARLLNFTTGAFHMAVF
                     TRPYFVWRFMQCLTDSIAIFTVTAATEREARAQLPHAHLIFVARIRQGVCHA"
     gene            1658..1978
                     /locus_tag="ACHKTW_004726"
     CDS             1658..1978
                     /locus_tag="ACHKTW_004726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001113145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6465612.1"
                     /translation="MPDMTNYQYLINPHFNCEHDIAKKVYSAADGATDNISMAVSSIG
                     SLMWYASENKEYDEKAMRIDMGNIGLLLAMLGQFDISLRCTIENAKDALNAIKKANTD
                     SNRG"
     gene            1985..2284
                     /locus_tag="ACHKTW_004727"
     CDS             1985..2284
                     /locus_tag="ACHKTW_004727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFO6465613.1"
                     /translation="MRTYLSGLTASGPARPEIRPGDIFRDNYGGVVEIKGVAERRITY
                     RREGYEYDCVMPVYQFRRDFSLVQAAPRSKPTSREKARANIQEIRKMLNVFRGKK"
     gene            2281..4029
                     /locus_tag="ACHKTW_004728"
     CDS             2281..4029
                     /locus_tag="ACHKTW_004728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5906 domain-containing protein"
                     /protein_id="HFO6465614.1"
                     /translation="MKLAPNVKKQPRGIKHKDTEVIIFAGSDAWAHAKQWQEQDGPAS
                     GDNVPPVWLGPNQLAELDALKIVPDGKKRVRLYQAGELDLVETKKIGQKLAAADIQDA
                     NFYPEGMHVQKCENWRRYLNAERENIAAGLTMPEQKNTQLAQMADSERAQLLAERFDG
                     VCVHPESEIVHVWRGGVWCPVSTMELSREMVAIYSEHRATFSKRVINNAVEALKVIAE
                     PMGEPSGDLLPFANGALDLKTGEFSPHTPENWITTHNGIEYTPPAPGENICDNAPNFH
                     KWLEHAAGKDPRKMMRICAALYMIMANRYDWQMFIEATGEGGSGKSTFTHIASLLAGK
                     QNTVSAEMTSLDDAGGRAQVVGSRLIVLADQPKYTGEGTGIKKITGGDPVEINPKYEK
                     RFTAVIRAVVLATNNNPMIFTERAGGVSRRRVIFRFDNIVREDEKDKDLPEKVAAEIP
                     VIIRRLLANFADPEKARALLLEQRDGDEALAIKQQTDPVIEFCQFLNFLEEARGLMMG
                     GGGDSVKYTTRNSLYRVYLAFMAYAGRSKPLNVAEFSKAMKPAAKVYGHEYITRRVKG
                     VTQTNAITTDDCDAFL"
     gene            4316..4583
                     /locus_tag="ACHKTW_004729"
                     /pseudo
     CDS             4316..4583
                     /locus_tag="ACHKTW_004729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707047.3"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4591..4863
                     /locus_tag="ACHKTW_004730"
     CDS             4591..4863
                     /locus_tag="ACHKTW_004730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFO6465615.1"
                     /translation="MIHDRIAEELEAKGFYRRASARWGEVMLLVETDKERHQVTMRRL
                     ECSRKAQKPPEPPDNFGDLRKAVDRTYAEMGIDGVSDEIWRNYPDS"
     gene            5104..5310
                     /locus_tag="ACHKTW_004731"
     CDS             5104..5310
                     /locus_tag="ACHKTW_004731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000796966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6465616.1"
                     /translation="MAEEKKGGVSVYISPDIVKALKERHQQNVKAGIAAGLDPLAMVE
                     PSTGWQVRAYLRAALGMNQVHGGE"
     gene            5310..6365
                     /locus_tag="ACHKTW_004732"
     CDS             5310..6365
                     /locus_tag="ACHKTW_004732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFO6465617.1"
                     /translation="MTVKAIALNTNQLFAYLNREDIAEFKFSPLFTALFFPNVATFNT
                     QDIMLDNLDIEEVTMSAFCSPMVGSQVQRDKGYETSIIRPGYMKPKHEIDPLKTIMRM
                     AGEDPAQLNDPTYRRMRLITGNMRRQINAIKARVEWLAVNAVTTGKNIIEGEGIERYE
                     IDWKIPEKNIIEQADGKKWSEQDKDIHDPIYDIELYADQAGCPANVMIMGVDVWRMLR
                     SFKKFRELYDLSRGSESAAELACKNLGEVVSFKGYLGDLALIVYSGKYTDSDGTEKYF
                     LEPDLLVLGNTNNKGLVAYGAIMEQEAVRTGATQNMYYPKNWIEDGDPAIEYVQTHSA
                     PQPVPADIRKFVTVKIG"
     gene            6377..6712
                     /locus_tag="ACHKTW_004733"
     CDS             6377..6712
                     /locus_tag="ACHKTW_004733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HFO6465618.1"
                     /translation="MNTPYVELFAGSQQVSTTLVHFAADAGVIQEFTPLMLADNGEFK
                     PWDGQESGKAVYLTSYPVDTSTQKSAQCYKTGIFNIAAVNWPESVDTDAKKCAAFAGS
                     GVSVQPLAR"
     gene            6725..7138
                     /locus_tag="ACHKTW_004734"
     CDS             6725..7138
                     /locus_tag="ACHKTW_004734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HFO6465619.1"
                     /translation="MATNESIMTLPLASKFKAEASAMADRGLSTYEAVYQLNKLEEQD
                     KPRADAIMALHEHKDYQPLLRAMANVPCISVDNAREILNMTIEQERPKVAPELTAAFE
                     NFMDMHSPQAVSAGMAYDGRNQGDDGDIDRILKTI"
     gene            7345..7887
                     /locus_tag="ACHKTW_004735"
     CDS             7345..7887
                     /locus_tag="ACHKTW_004735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFO6465620.1"
                     /translation="MKSHLMNKKNMAKSCRVSATAFDKWGVTPVERKGREAFYDVASV
                     IENRVNNAISQLINDKGEIDDDELLRVRIRLLTAQAEAQELKNDRDRGDVIDTEFCLY
                     ALSKLASQISSIMDSLPLTMQRSFPQITPAMLDRLKREVVKACNASARVADNLPQILA
                     DYLKETTGNVPEKLLQKKGE"
BASE COUNT         2202 a         1844 c         2095 g         1805 t
ORIGIN      
        1 ccgatatgga gccgcctttc tgaacaatta acccgctgcg ccttatgtgt atgtgatccc
       61 aaacataagc acggggatga tagccgctat caggctggtg ggcaatgcaa tcagtctggt
      121 tcagttcgtt gccatacctg caatgagcgc ttttccctgt actctttaag gaattgctca
      181 agggcaaaag cacatggtgc gaatctttct gattcatgct ctatctttct gcgccgtctt
      241 tttcgtgcag gtgatgtttt ttcggttgat tcttcgttgg tcattgtgtg cgcctgtaaa
      301 gcaatgcgcc ggagtacctc acaccacggc gctgatagtt tttatccttt gggttctatg
      361 ccgcgttgct gtaattcttt gcggattatt cgctttatcc aggcagcaag agaatcatcg
      421 ccgtcctgtt gttgtgcctt ttccatcttt tcccgtaagt caggatctaa acggaactgg
      481 aacggaggat taccacgcct ttcgtttttg tgtgttgaca cgttaattac acctcatgta
      541 atgtatttat gtgtaatgac acattacaca caatcaacac aaaaagcaaa gccccgcact
      601 gtaggagcag taacggggct tctaaccaac aacgtaaact aggagccgtt atggttgctg
      661 taaatcatat accacacctt gtacacacac aaacggcctt tgtgtggcgt tttctggccc
      721 tgaatatcgg agaaaaaaac cgcttgatcg ccaccagcaa aaatggctat gattcccgcg
      781 cacctcataa aacgggtgtc gggattagcc tcctgaaaac aactgaagcg cacagcacgc
      841 gccccgcgtg tttttttgtg ccgcatagtc acaccttatc aatggtgggc tgtacggggg
      901 cggagcaatc cgcgccggtt tcttcagtgt ccggtaaggc taaccctgta cagtccgcca
      961 ccagcgaaat tagccttttg cgtggcggtt atcttaacca cactgaggag gctgccaaca
     1021 tggctactat ccctaccctt gtacattctc aaaccgcatt tatctggcgc tttatcatct
     1081 ttggcgcgtc agaatctcaa atcatccacg taaccgcctg gacggaacgc gaagcgcgta
     1141 gccgttgccc gtcaggttgt gttgctgtat tcgccgcccg tattcgtcag gggtcacatc
     1201 atgcgtaaaa accgcttgca aaaaattata acggggctgt atgcttcccc agtcgcccac
     1261 atggcgaccg ggtttagcag cctgaatact acagcggaca gccgcttaca ttccgatatg
     1321 cggttttttt gtgtccgtaa acctacccat acccgcatta tggcggggcg taacggggga
     1381 gcctttgcgc tcgctggttc ctgtagtgcc agtctgctaa ccctgttacg tctcgccacc
     1441 ccgtttagca gcgtagtagc gagactcctt aatttcacta caggagcctt tcacatggct
     1501 gtattcacac gcccttattt tgtctggcgc tttatgcagt gcctgacaga cagcattgca
     1561 atattcaccg ttaccgcagc cactgaacgc gaagcccgcg cccagttgcc gcacgcacat
     1621 ctgatttttg tcgcccgtat tcgtcagggg gtgtgccatg cctgacatga ccaattacca
     1681 gtacctgatt aatccgcatt ttaactgtga gcatgatatt gctaaaaagg tttattccgc
     1741 tgcggatggg gctactgaca atatatcaat ggctgtttcg tcaattggta gcctgatgtg
     1801 gtatgcgtca gaaaataagg aatatgacga aaaggccatg cgcattgata tgggtaatat
     1861 cggtttgtta ctggcaatgc ttgggcagtt tgatatttcg ttacggtgca ccattgaaaa
     1921 tgccaaagat gcattaaatg ctataaagaa agcgaatact gattcaaatc ggggataaat
     1981 aatcatgaga acatatttat ctggcttgac tgccagcggc cccgctcggc ctgaaatccg
     2041 ccccggagat attttcagag ataactacgg tggcgttgtt gagattaaag gcgtggcaga
     2101 acggcgcatt acctaccgcc gtgaaggcta cgaatatgat tgcgtgatgc ctgtttatca
     2161 gttccggcgt gatttttctc tggtacaggc agcgccccgc agtaaaccaa ccagcaggga
     2221 gaaagcacgc gccaatattc aggaaataag aaagatgttg aatgtattca ggggtaaaaa
     2281 atgaaactgg caccgaacgt aaaaaaacaa ccacgcggaa taaaacacaa agacacagag
     2341 gtaattattt tcgcgggtag tgatgcgtgg gcacatgcaa aacagtggca ggagcaggac
     2401 ggccccgcat ccggcgataa tgtgccgcct gtgtggcttg ggccaaatca gcttgccgaa
     2461 cttgatgcac tgaaaattgt tcctgatgga aaaaaacgcg taaggctgta ccaggccgga
     2521 gaactggatt tggtggagac caaaaagatt ggtcagaagc tggcggcggc agatattcag
     2581 gacgcaaatt tttaccccga aggaatgcac gtccagaagt gtgaaaactg gcggcgctat
     2641 ctgaatgctg agcgtgaaaa tattgccgca gggcttacca tgccggagca gaaaaatacg
     2701 caactggcac aaatggcaga cagtgagcgc gcgcagctgc ttgccgagcg ctttgatggc
     2761 gtttgtgtgc atccggaaag tgaaatcgtt cacgtatggc gcggcggggt atggtgtccg
     2821 gtcagcacaa tggaacttag ccgcgaaatg gtggcgatct attcagagca cagggccact
     2881 ttcagcaagc gcgtaatcaa taacgccgtg gaagcgttaa aagttattgc cgaaccaatg
     2941 ggcgagccgt ccggcgattt gttgccgttc gccaatggtg cgcttgacct gaaaacgggg
     3001 gaattttccc cgcacacgcc ggagaactgg atcaccacgc acaacggcat tgagtacacg
     3061 ccaccagcac caggggagaa tatctgcgac aacgcgccaa actttcataa atggcttgag
     3121 cacgcagccg gaaaagaccc gcgcaagatg atgcgtatat gtgccgcgct gtacatgatt
     3181 atggcgaacc ggtacgactg gcagatgttt attgaggcca ccggagaggg cggtagcggt
     3241 aaaagtacat tcacgcacat agccagcctt ctggcaggga aacagaacac ggtaagcgct
     3301 gaaatgacct cacttgatga tgctggtgga cgtgcgcagg ttgtcgggag tcgtcttatc
     3361 gtcctggctg accagccgaa atatacaggc gaaggaacgg gcatcaagaa aatcacgggc
     3421 ggcgaccccg tggaaataaa cccgaaatat gagaagcgtt ttacggcggt aatcagggcg
     3481 gtggtgctgg ctaccaacaa taacccgatg atattcaccg agcgggccgg aggtgtgtca
     3541 cgccgtcggg tgattttccg gttcgacaac attgtaaggg aggacgaaaa agacaaggat
     3601 ttgccggaga aggtcgcggc tgaaatcccc gtaattatcc gtcgcttgct ggctaatttt
     3661 gccgaccctg aaaaggcacg ggctttatta ctggaacagc gcgacggtga tgaagctctg
     3721 gcaataaagc aacagacgga tccggttatt gagttttgcc agttcctgaa ttttctggag
     3781 gaagcgcgcg gcctgatgat gggtggcggt ggcgattcag tgaagtacac gaccagaaac
     3841 agcctttacc gcgtctatct ggcgtttatg gcatacgcag gcaggagcaa accgctaaac
     3901 gtggctgagt tcagcaaggc catgaagcca gcggcaaaag tttacggaca tgaatatatt
     3961 acgcggagag ttaagggagt aacgcagacc aacgcaatta caactgatga ttgtgacgcg
     4021 tttttataat tttttgtaaa agccctctac cccatctacc tgaatgaaat aaacgcatat
     4081 tattcaacat gataagtggg tagagggcta ggtagaaggc taataaaacc tctctacctc
     4141 ttctaccatg attaagatcg tttgtgaggg gcgggtagag gatgggtaga gggccacgaa
     4201 agccctctac ccatctgaaa gccgcgccat tactgacatg aaagatgatt aggtagagag
     4261 gtagaggagg tgcaccacaa cctaaaactt tttaaacacg agggtaaaaa taaaaatgca
     4321 cacatcagga agatttaaca aatcactcaa aaaacgcaga gacagaacag aaccgaaata
     4381 tcgcgcgtta gacatgacag agcacgcttt aaaggtggca atcagaacga tagaccgcca
     4441 cgcgggggga aggatacgcg aaagcacatc ccgaactgat aagcgcattc atgaccacgg
     4501 cggcggcaaa ctttgccacg ctgacagaac gggagattgc cgaagcggaa caggtaacaa
     4561 ccatcaacgt aaaaaccgga tagtcaggcg atgatacatg accgcatagc ggaggaactc
     4621 gaggcaaaag gcttttaccg gagggcatcg gcgcgatggg gtgaagtcat gctgctggtg
     4681 gagacagaca aggaacggca tcaggttacg atgcgacggc tggaatgttc caggaaggca
     4741 cagaagccac cggagccgcc ggataacttc ggagacctga gaaaggcagt agatcgcact
     4801 tatgctgaaa tgggtataga tggtgtaagc gatgaaatat ggcgtaatta cccagacagc
     4861 taatcaacag ccggagtaat ccggcttttt tgtacccaaa aaaagcccga taagtacagg
     4921 aggcatctta tcgggctttt gcatatgagg ttttttttgg tgcactgaca cacatgatcg
     4981 ggatcatcat ttcataattt gcaacacaac tcaacatcat tgcataaaat gcaatcatga
     5041 ttataatcag atctggatga acatccagtt atgatttttt aagtcaaaga ggaatttctt
     5101 actatggctg aagagaaaaa aggcggtgtt tcggtgtaca taagccccga catcgtgaag
     5161 gcgctcaagg aacgccacca gcagaacgta aaagcaggca ttgcggcagg acttgatccg
     5221 ctggcgatgg ttgagccgtc aacaggctgg caggtacgcg cctatttacg cgcggcgctg
     5281 ggtatgaatc aggttcacgg gggtgaataa tgacagtaaa agcaatagca cttaacacta
     5341 accagctttt tgcgtacctg aatcgcgagg atattgcgga atttaaattc agtccgctgt
     5401 ttaccgcgct gtttttcccg aacgtggcga cattcaacac acaggacatc atgttagata
     5461 acctggatat tgaagaagtc actatgtcgg cgttttgttc gcctatggtg ggtagccagg
     5521 ttcagcgcga taaagggtac gaaacaagca ttattcgccc tggctacatg aagccaaaac
     5581 acgaaatcga tccattaaaa acaataatgc gcatggctgg agaagatcca gcacagctta
     5641 acgaccctac ctaccgccgt atgcgcctga ttactggcaa catgcgccgc cagataaacg
     5701 ccattaaagc gcgcgtggaa tggctggcgg taaatgcggt aacgaccgga aaaaacatca
     5761 ttgagggcga aggcatagag cgctatgaaa tcgactggaa gataccggaa aaaaacatca
     5821 tagagcaggc cgacggtaaa aaatggtccg agcaggataa agacatacac gatccaatct
     5881 atgacattga gctatacgca gatcaggcag gttgccccgc caacgtcatg attatgggcg
     5941 ttgatgtatg gcgcatgtta cgcagcttta aaaaattccg tgagctgtac gatctttccc
     6001 gtggttcaga atccgccgca gagctggcat gtaaaaacct gggcgaagtg gtgagcttta
     6061 agggctatct tggtgatctg gcccttatcg tctattccgg caaatacact gacagcgacg
     6121 gtaccgaaaa atatttcctt gagcctgatt tgctggtcct gggcaacacc aacaataaag
     6181 ggctggtggc ctatggtgcg attatggaac aggaagcggt cagaactggc gcaacgcaaa
     6241 acatgtacta cccgaaaaac tggattgagg acggcgatcc ggcgattgag tacgtgcaga
     6301 cacacagcgc accgcagccg gtaccggcag atattcgcaa atttgttacc gtcaaaattg
     6361 gttaacgggg gattctatga acactccata cgttgagtta tttgcaggca gtcagcaggt
     6421 atcaacgacg ctggtacatt ttgccgctga tgctggcgtt attcaggaat ttaccccgct
     6481 gatgctggcg gacaatggcg agtttaagcc gtgggatggt caggaatctg gcaaggctgt
     6541 ttatctgacc tcgtaccccg ttgacacgtc gacgcagaaa tcagcacagt gctataagac
     6601 ggggatattt aatatcgccg ccgttaactg gcctgagagc gtcgacaccg atgcgaaaaa
     6661 atgcgccgcc tttgcgggtt ccggcgtatc cgttcagccg ctggcccgat aagcaggggt
     6721 aacgatggca acgaatgaaa gcatcatgac gctaccgctg gcgagtaaat ttaaagccga
     6781 agcgagtgca atggctgaca gaggtttatc aacctatgag gccgtatatc aactaaacaa
     6841 actggaagag caggacaagc cgcgcgctga tgcgattatg gcgcttcatg agcataaaga
     6901 ctatcagccg ctgttacgtg caatggcaaa cgtgccatgt attagcgtcg ataatgctcg
     6961 tgaaatcctg aacatgacca tagagcagga gcgcccaaag gttgcaccag agcttaccgc
     7021 agcctttgaa aactttatgg acatgcacag cccgcaagcc gtatcagctg gcatggcata
     7081 cgatggcaga aaccagggcg atgacggcga catcgatcgc atactgaaaa ccatctgaga
     7141 caaggccgga gaaatccggc tttttttacg ggtcctttcc ggcatatgga cccgttacgg
     7201 ggcggcgacc tcgcggtttt tcgctattta tgagcctttt caggggggtg gtggtggttt
     7261 tgttgtttga tctatcttta tgaatgaaaa gggaaagatg caagcaatac accaacctga
     7321 agcagtaatt aagttggtgt attaatgaaa tctcatctga tgaacaaaaa aaacatggcg
     7381 aaaagctgcc gtgtaagtgc gacagcgttc gacaagtggg gagtaactcc cgttgagcgt
     7441 aaaggccgtg aggcgtttta tgatgttgcc agcgtgatag aaaatagggt taacaatgca
     7501 attagccagc ttataaacga caaaggcgag attgacgatg atgaactttt acgagtcagg
     7561 atcagattac tgacagcaca ggcagaggcg caggaactta aaaacgatcg cgatcgcggt
     7621 gacgtgattg atactgagtt ttgcctgtac gcgctttcaa agctggcgag tcagatttca
     7681 tcaatcatgg acagcctccc gcttactatg caaaggagct tcccacagat tacccccgcc
     7741 atgctggata ggcttaaaag ggaagtggtt aaagcctgta atgccagtgc cagagttgcc
     7801 gacaacctcc cacagatact ggctgattac ttgaaagaaa caaccggaaa cgtaccggaa
     7861 aagttgctac agaagaaagg cgagtaacag acgcgcaatt attgaacaaa actgagaaac
     7921 gacatgaaag cgtcataaat cgccat
//
LOCUS       DAZFQD010000192         7921 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.192, whole genome shotgun sequence.
ACCESSION   DAZFQD010000192 DAZFQD010000000
VERSION     DAZFQD010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7921
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.192"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..542)
                     /gene="zinT"
                     /locus_tag="ACHKTW_004736"
     CDS             complement(<1..542)
                     /gene="zinT"
                     /locus_tag="ACHKTW_004736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HFO6465621.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDANVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKTKTFAEIKDYYHKGYA
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIF"
     gene            complement(799..1434)
                     /gene="msrQ"
                     /locus_tag="ACHKTW_004737"
     CDS             complement(799..1434)
                     /gene="msrQ"
                     /locus_tag="ACHKTW_004737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HFO6465622.1"
                     /translation="MRLTAKQVTWLKVSLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLATLLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQAMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLRSLFNRLRKQVHNKLSV"
     gene            complement(1435..2439)
                     /gene="msrP"
                     /locus_tag="ACHKTW_004738"
     CDS             complement(1435..2439)
                     /gene="msrP"
                     /locus_tag="ACHKTW_004738"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HFO6465623.1"
                     /translation="MKKNQFLKESDVTAESIFFMKRRQVLKALGISAAALSLPHAAHA
                     DLLSWFKGNDRPPAPAGKPLEFSKPGAWQNNLPLTPVDKVSGYNNFYEFGLDKADPAA
                     NAGSLKTDPWTLKISGEVAKPLTLDHDDLTRRFPLEERIYRMRCVEAWSMVVPWIGFP
                     LHKLLALAEPTSNAKYVAFETIYAPEQMPGQQDRFIGGGLKYPYVEGLRLDEAMHPLT
                     LMTVGVYGKALPPQNGAPVRLIVPWKYGFKGIKSIVSIKLTRERPPTTWNLAAPDEYG
                     FYANVNPHVDHPRWSQATERFIGSGGILDVQRQPTLLFNGYADQVASLYRGLDLRENF
                     "
     gene            complement(2548..2961)
                     /gene="hiuH"
                     /locus_tag="ACHKTW_004739"
     CDS             complement(2548..2961)
                     /gene="hiuH"
                     /locus_tag="ACHKTW_004739"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HFO6465624.1"
                     /translation="MLKRYLVLSVVTAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            3094..3765
                     /gene="hprR"
                     /locus_tag="ACHKTW_004740"
                     /pseudo
     CDS             3094..3765
                     /gene="hprR"
                     /locus_tag="ACHKTW_004740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
     gene            3765..5123
                     /gene="hprS"
                     /locus_tag="ACHKTW_004741"
     CDS             3765..5123
                     /gene="hprS"
                     /locus_tag="ACHKTW_004741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HFO6465625.1"
                     /translation="MKRLSITVRLTLLFILLLSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHGDGINKIVNRTNVSDGMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIVLCSVLSPLLIRTGLREIKKLSGVTEALNYNDSREPVEVSALPRELKPL
                     GQALNKMHQALIKDFERLSQFADDLAHELRTPINALLGQNQVTLSQTRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLNKEVENLLDYLEYLSDEKEICF
                     KVECNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIHITSFLDTNGYLNIDVASPGTK
                     IHEPEKLFRRFWRGDNSRHSVGQGLGLSLVKAIAELHGGSATYHYLNKHNVFRIMLPQ
                     RN"
     gene            complement(5231..6082)
                     /gene="hchA"
                     /locus_tag="ACHKTW_004742"
     CDS             complement(5231..6082)
                     /gene="hchA"
                     /locus_tag="ACHKTW_004742"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000218213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036524 - protein deglycase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase III HchA"
                     /protein_id="HFO6465626.1"
                     /translation="MTVQTSKNPQVDIAEDNAFFPSEYSLSQYTSPVSDLDGVDYPKP
                     YRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLHAAGFEFEVATISGLMT
                     KFEYWAMPHKDEKVMPFFEQHKSLFRNPKKLADVVASLNADSEYAAIFVPGGHGALIG
                     LPESQDVAAALQWAIKNDRFVISLCHGPAAFLALRHGDNPLNGYSICAFPDAADKQTP
                     DIGYMPGHLTWYFGEELKKMGMKIINDDITGRVHKDRKLLTGDSPFAANALGKLAAQE
                     MLAAYVD"
     gene            complement(6674..7837)
                     /gene="ompC"
                     /locus_tag="ACHKTW_004743"
     CDS             complement(6674..7837)
                     /gene="ompC"
                     /locus_tag="ACHKTW_004743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020218947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HFO6465627.1"
                     /translation="MKRKVLAMLVPALLVAGAANAAEIYNKNGNKLDLYGKVDARHTF
                     SDNPGDDGDETIIELGFKGETQITDQLTGYGQALTKTKASDTEGSDNTYVKLAFAGLK
                     FGEMGSFDYGRNYGVIYDVEAWTDMLPVFGGDSYTWTDNFMAGRANGVATYRNSDFFG
                     LVEGLNFALQYQGNNEGSNAGEDQEGTKNGHEDVRFQNGDGFGLSTSYDFDFGLSLGA
                     AYSNSDRTNSQVALGGYHYNEYSKFAGGDTAEAWTFGAKYDANNVYLAMMYAETRNMT
                     PYGNVGIANKTQNFEAVAQYQFDFGLRPSLAYVYSKGKDLGGNDYNNNGHQEYVDQDL
                     VNYVEIGATYYFNKNFSTYVDYKINLLDKDDDFYDNNGIATDDVVGVGLVYQF"
BASE COUNT         2198 a         1663 c         1822 g         2238 t
ORIGIN      
        1 ataaaaatgt gaaaatgaga tgattttcgt ggtgcaatta tatgatcgct aaattgtata
       61 tatttagggg ctttgctttc aggatcttta cattcgaata agtaacgaac gcctttcttg
      121 cctgatttat aggtgagtat tttgtatcca tcgtaatcat atttacagga tgttgtttca
      181 ttatttctat ggaattcaac aatgccgtcc tcaatgccaa tcatctcgat atctgttgca
      241 taacctttgt gataataatc tttaatttca gcaaatgttt tagttttatc tgcatccgct
      301 ttcttctgaa agacggggtc aagtttgcca ctttgcagta aaggataaac ggattgccag
      361 actccatccc agtcactgag cgttcggttt tgtacattgg catcatcaaa aacaccatta
      421 gcagcttttt gttcgacctc tgttaagggt ttgccgtgtg agtgatgacc atgcgaaaag
      481 gcaggagcgc taacaataaa gacacctaaa gcaacagcca gtttgtgaag acgaatcgcc
      541 aaaacagttt cctccagagt cattgtatat gatgtgtaat gttatattgt aacatatgac
      601 aaaatgcaat aactcttagg agatgagagc atagttttct caggtatact tatctatctt
      661 attgaaataa aatgaattat gttgataatt tgaggttgat gacaaacgaa acataacctg
      721 atgtgatctg cgtaggccgg atagggcgct cgcgccgcat ccggcaggga taacgcgtac
      781 tttgtcggta atatgaagtt atacagataa tttgttgtga acctgcttgc gtaaccggtt
      841 aaaaagggaa cgcaacttct tataccgtaa ggctaaaagc agtacagcca gcccagcgta
      901 gatgagcggc tgcggtgaaa taatcttcac tgaccacaga taatgtattg gggccaggat
      961 cgcgacaaga tagacgaaat tatgcaactg ttgccagtgc ttgccgagtt ttcgctgcat
     1021 cgcctgggtt gaagtaaacg ctaaagcaag caaaataacc cagctgataa ttcctaacgt
     1081 taaataaggc cgggtaatta actccttgcc taataacgcc agattattca cgcccagctc
     1141 cagcaatgcg taactggtta aatgcagtgt cgcccaggca aagcaccata agcctaacag
     1201 gcggcgagtg cgtatcaata acggctgttt tgcgtagcgt gccagaggtg tgattaacaa
     1261 ggtcgccagc aaaaatttca gtgcagtgcg accggtaaaa tgctgaatat ctttcaccgg
     1321 atcggcaccc agtccaccgt gattgatcgc ccagaccagc cagagaaacg gcaacaatcc
     1381 ggcaagatgc aggctaactt tcagccatgt cacctgtttt gctgtcagac gcattcagaa
     1441 attctcccgc aaatccaggc cacgatacag cgatgccacc tggtcggcgt aaccattaaa
     1501 cagtagcgtt ggctggcgct gaacatcgag gatgccgcct gaaccaataa atcgttcggt
     1561 agcctgtgac cagcgcgggt gatcaacatg cggattaacg ttggcgtaaa aaccgtattc
     1621 gtcaggcgct gccagattcc aggtggttgg cggacgctcg cgggtcagct taatactgac
     1681 tatcgattta atccctttaa agccatattt ccacggcaca atcagtcgta ccggcgcgcc
     1741 attttgtggc ggtaacgcct tgccataaac acccacggtc atcagtgtga gcggatgcat
     1801 tgcttcgtcg agacgcaatc cttcgacata aggatatttc agcccgccgc cgataaagcg
     1861 gtcctgctgg ccaggcatct gttccggtgc ataaattgtt tcgaaagcga catacttcgc
     1921 attgctggtg ggttcggcaa gcgccagcaa tttgtgcagc ggaaaaccaa tccacggcac
     1981 caccatcgac catgcctcca cgcagcgcat acgataaata cgctcttcca gcgggaagcg
     2041 acgggttaaa tcatcgtgat cgagggtcaa tggttttgcc acttcgccgc tgattttcag
     2101 tgtccatgga tcggttttca ggctaccagc attagcggcg ggatcggctt tatccagccc
     2161 gaattcatag aagttgttat aaccggagac tttatctact ggcgtcagtg gcaggttatt
     2221 ttgccaggcg ccaggcttgc tgaactccag cggttttccg gcgggggcgg gcgggcgatc
     2281 gttcccttta aaccagctaa gcagatcggc atgcgcagcg tgaggcaaag aaagtgcagc
     2341 tgcgctgatg cccagtgctt ttaacacctg tcgacgcttc ataaagaata tcgactcggc
     2401 cgtaacatct gattctttta aaaattgatt ctttttcata agccatcaca cttttttgtc
     2461 gttagccagc aagagccgtt tactgttaat ctgtcaggga gtgtcctccc taacagtagc
     2521 agagatctgc ttttacatat ttatgcatta actgccacga taggttgaat acccatattg
     2581 gctaagtaat aaaggcacat gataatgctc gttcacttta ttaatatgaa attcaacggg
     2641 gatctcaggg aagaaacttt caagattttg tttcttgaaa tagtccccgg ttttaaatac
     2701 gacacggtaa tcgcccgtag ttgcagtttg ctcgggccac agtgccttaa ttcgtccatc
     2761 cttatctgtt ttggcggtat taagttgtaa ccagccgttg tccgctttct tttcaagagt
     2821 gactgtcacg tcggcagcgg gttttccggt ttgctggttc aaaatgtgca cgctaagaat
     2881 gttttgttgt gcggcataaa ccaaagaagg taatgaaaat gctgccgtta ctacggagag
     2941 tactaaataa cgctttaaca tgtttatatc cttgtcatgt gaatgagttt tgaggatagc
     3001 gaaaaagcgg ccagggtaac cttacagcag cattacaatt ttgtaatgaa ccaggctgtt
     3061 tctataacat atgatttatg gcatattatt ttcatgaaga ttttacttat tgaagataat
     3121 caaaggaccc aggagtgggt aacgcagggg ctttccgaag cgggttatgt cattgatgcc
     3181 gtttctgatg gcagagatgg gctttatctt gcgctgaagg atgattatgc attgatcatt
     3241 ctggatatta tgcttccggg tatggatggc tggcagatct tacaaacgtt aagaacagca
     3301 aagcaaaccc ctgttatttg ccttactgca agggattctg tcgatgacag agtcagaggg
     3361 ctggacagtg gagcaaatga ttatctggta aaaccttttt cattttctga gttgctggca
     3421 agggttcggg cacaattaag gcaacatcac gctttgaatt caacattata aatcagtggc
     3481 ttaagaatgg actctgtaag tcaaagtgtg agcagggaca atatcagtat tacactgacg
     3541 cgcaaggagt ttcagttact ttggctactg gcctccagag ctggcgaaat tatacccaga
     3601 acggttattg cgagtgaaat ttggggaatc aactttgata gtgataccaa tacggtggat
     3661 gtcgccattc gcaggctccg cgcaaaagtt gatgatcctt ttcctgaaaa gctaatcgcc
     3721 acaatccggg ggatgggcta ttcattcgta gcggtaaaaa aataatgaaa agattatcta
     3781 taaccgtccg tttaaccttg ctttttatat tgctgctgtc tgttgctggc gctggaattg
     3841 tctggactct ctataatggc ctggcaagtg agttgaaatg gcgcgatgat acaacactca
     3901 ttaaccggac agcacagatc aagcagttgt taattgatgg ggtaaatcca gatacgttac
     3961 ctgtgtactt taaccggatg atggatgtta gtcaggatat cttgatcatt catggtgatg
     4021 gcatcaataa aattgttaac cggacaaatg tcagtgatgg catgttaaat aacatacctg
     4081 ctagtgagac aatcagcgca gctggcattt acagaagcat tattaatgac acagagatag
     4141 atgctttacg aattaatatt gatgaagttt cgccatcatt aacggttact gtggctaaat
     4201 tggcttcagc cagacataac atgcttgaac agtataaaat taatagcatt ataatttgca
     4261 ttgtcgccat tgtactttgc tcagtattaa gtccgctgtt aatcagaacg gggttacgag
     4321 agatcaaaaa gttgagtggt gtaacggaag cgctgaatta taacgatagc cgggagcctg
     4381 ttgaggttag cgcattaccg agagaactaa aacctcttgg gcaggcgttg aataaaatgc
     4441 atcaagcctt aatcaaagat tttgagcgac taagtcagtt tgctgacgat ctcgctcatg
     4501 aacttagaac gccaattaat gcattactgg gtcagaatca ggttacgctc agtcaaacca
     4561 gaagtatcgc tgaatatcaa aaaacaattg ccggtaacat tgaagagctg gaaaatattt
     4621 cgcggttaac agagaacata ctgtttcttg ccagggcaga taaaaacaat gttttggtga
     4681 aactggactc gctttctctc aataaggaag tcgaaaattt gttggactat cttgaatacc
     4741 tttcagacga gaaagagatt tgctttaagg tcgagtgcaa tcagcaaatc tttgcggata
     4801 aaattttact gcaacgaatg ttatcgaatc ttattgttaa cgccattcga tattcgccag
     4861 aaaaatcgcg cattcatata accagttttc ttgataccaa cggctatctt aatattgatg
     4921 tcgccagtcc tggaacgaaa attcatgagc ctgaaaaact cttccgtaga ttttggcggg
     4981 gagataattc gcgtcattcc gtaggtcagg gacttggtct ttctttagtc aaagcgattg
     5041 ccgaattgca tgggggaagt gctacgtatc actatctcaa taagcataat gtgttccgga
     5101 ttatgttacc gcaaagaaat taacataggg cttcagtacg ccataaatca accatgaaaa
     5161 aacgcagcca cgtcgtgagt attaacgtgg ctacgattga ttatgcgttt acattcaaac
     5221 gtaacaggga ttaatccaca taagctgcca gcatttcctg cgccgccagt ttacccaacg
     5281 cattcgctgc gaaaggactg tcgccggtga gaagtttacg gtccttatgt actcgcccgg
     5341 tgatgtcgtc attaatgatt ttcatgccca ttttcttcag ctcttcgccg aagtaccagg
     5401 tgagatgacc tggcatatag ccaatatctg gcgtttgttt gtctgcggcg tctgggaatg
     5461 cgcaaatgga ataaccattc agtgggttat cgccgtggcg aagcgccaga aaagccgccg
     5521 ggccgtggca aagggagata acaaaacggt catttttgat tgcccactgt aaagcggcag
     5581 ctacgtcctg gctttcaggt aaaccaataa gtgcgccatg accaccagga acaaagattg
     5641 ctgcatattc gctatcagcg ttgaggctgg cgacaacatc cgcgagtttc ttcggattgc
     5701 ggaacaacga tttatgctgc tcaaagaatg gcattacttt ttcatctttg tgcggcatag
     5761 cccagtattc aaacttggtc atcagaccgg aaatggtcgc cacttcgaat tcgaaacctg
     5821 cagcatggag atgatacaac ggcagcaacg tttcaatcgg atggttaccg gtcgagaaca
     5881 gttttccgtt atcggttggc aaataacgtt cgtctgcggc gatcaccaga attttatgtt
     5941 taccgcgata cggttttgga tagtccacgc catcaagatc agagacagga ctggtatatt
     6001 ggctaagcga atattctgaa gggaagaatg cattatcttc agcaatatcg acctgcggat
     6061 ttttacttgt ttgaacagtc atagtgtatt ccttattgtt gcttagttag ggtagtcact
     6121 atatttgcga ctgagctggt cgctattttt aaccaggctg gaaaattgag tcaatatatc
     6181 gaaagcggta gtcggcgggt gtaagtatgt aatgatgatg ctgcactggt gttgaatatt
     6241 attcacgcca aattttcctt attgagcaac gtcgtcgcaa gtggcctcaa ttattttaga
     6301 ccattatcag aggtacgggg cgacggggag agatttagtg ctgtggaata ttgtggtgct
     6361 gatgaatatt tatatcccgg attggtagag aaaaagctta tttcatgcac tatttttgca
     6421 aatatttcta tatgggaggc ggttttgttg cagattaatt cataaagaaa tgcatttggg
     6481 tcttggctgg aagagtcgct ggtagatgtt attgtgccag caggggcagg gtaaggtgct
     6541 gtatcgatat gcattggcaa ttgcgtgtgc cgcagatgga gatgaaatgc caataaaaaa
     6601 gcctgaattc gatgagggac gaactcaggc tagtgtttag ttttacagta acacgccgca
     6661 gcacgttggc tgattagaac tggtagacta agcccacacc aacaacgtca tctgttgcga
     6721 tgccattgtt gtcatagaaa tcgtcatctt tatccaacaa gtttatttta tagtcaacat
     6781 atgttgagaa gttcttattg aaatagtagg tcgctccaat ttcaacatag ttgaccagat
     6841 cctgatcaac atactcttga tgaccattgt tgttatagtc gttaccgccg aggtctttgc
     6901 cttttgaata aacatatgca agagatgggc gcagaccaaa atcgaactga tactgtgcta
     6961 cagcttcaaa gttctgggtt ttatttgcga tacccacatt accatatggt gtcatattgc
     7021 gggtttcagc atacatcatt gcaagataaa cattgttggc gtcgtatttt gcaccaaatg
     7081 tccatgcttc agctgtatca ccaccagcaa atttgctgta ttcattgtag tgatacccgc
     7141 caagagctac ttgagaattg gtacggtcag agtttgagta agcggcacct aagctcaaac
     7201 cgaagtcaaa gtcatatgaa gtggaaagac caaaaccatc accgttctgg aagcgaacgt
     7261 cttcgtgacc atttttagta ccttcttgat cttctcctgc attgctgcct tcgttgttac
     7321 cttgatactg caatgcaaag ttcagacctt ccaccagacc gaagaaatca ctattgcggt
     7381 aagtagctac cccatttgca cggcctgcca tgaagttatc agtccaggta taggagtcgc
     7441 cgccgaatac aggaagcata tctgtccagg cttcaacgtc gtaaatcaca ccatagttac
     7501 gaccatagtc aaaggagccc atctcgccga acttcagacc tgcaaaagca agtttgacat
     7561 aagtattatc agaaccttca gtgtcgctag cctttgtttt ggtcagtgct tgaccataac
     7621 cagttaactg atctgtaatt tgggtctcac ctttgaagcc taactcgatg atagtttcat
     7681 cgccgtcatc gccagggtta tcggaaaaag tatgacgagc atcaactttt ccgtacagat
     7741 ccagtttatt gccattttta ttataaattt cagccgcatt tgctgcgcca gcaactaata
     7801 acgccgggac cagcattgcc agaacttttc ttttcattat gtattccctt gtgattataa
     7861 tcttcatgaa tatatcaata agtgccgtta tccaaaaaaa agcacatttg gatactattc
     7921 t
//
LOCUS       DAZFQD010000193         7706 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.193, whole genome shotgun sequence.
ACCESSION   DAZFQD010000193 DAZFQD010000000
VERSION     DAZFQD010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7706
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.193"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(33..983)
                     /gene="cbl"
                     /locus_tag="ACHKTW_004744"
     CDS             complement(33..983)
                     /gene="cbl"
                     /locus_tag="ACHKTW_004744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HFO6465628.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEL
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGTPQEIATLLQNGEADIGIASERLSNDPQLVA
                     FPWFRWHHSLLVPLDHPLTQITPLTLESIAKWPLITYRRGITGRSRIDDAFARKGLLA
                     DIVLSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEENLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
     gene            complement(1085..2002)
                     /gene="nac"
                     /locus_tag="ACHKTW_004745"
     CDS             complement(1085..2002)
                     /gene="nac"
                     /locus_tag="ACHKTW_004745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HFO6465629.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVATLEGELN
                     QQLLIRKKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQALSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEIVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQSVDVNAIAQMNLFLPSDYSAIRLRVDEAFSLRRLTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQWQLVS"
     gene            2329..2404
                     /locus_tag="ACHKTW_004746"
     tRNA            2329..2404
                     /locus_tag="ACHKTW_004746"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2362..2364,aa:Asn,seq:gtt)
     gene            complement(2460..3395)
                     /gene="ldtA"
                     /locus_tag="ACHKTW_004747"
     CDS             complement(2460..3395)
                     /gene="ldtA"
                     /locus_tag="ACHKTW_004747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFO6465630.1"
                     /translation="MMRRVNIFCSFALLFASHTSLAVTYPLPPEGSRLVGQSFTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKSGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNTRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSNWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFISGQEVDVNRANAALQRRSGMPVQISSGSRQMF"
     gene            complement(3457..4536)
                     /gene="cobT"
                     /locus_tag="ACHKTW_004748"
     CDS             complement(3457..4536)
                     /gene="cobT"
                     /locus_tag="ACHKTW_004748"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HFO6465631.1"
                     /translation="MQTLADLLNTIPAIDPAAMSRAQRHIDGLLKPVGSLGRLEALAI
                     QLAGMLGLNGVPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVYVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRHQAEKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRAPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGMAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMEMRLGEGSGAALAMPIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            complement(4548..5291)
                     /gene="cobS"
                     /locus_tag="ACHKTW_004749"
     CDS             complement(4548..5291)
                     /gene="cobS"
                     /locus_tag="ACHKTW_004749"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HFO6465632.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGVPLAALFSVLVLALMTGGFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLPGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            complement(5288..5833)
                     /gene="cobU"
                     /locus_tag="ACHKTW_004750"
     CDS             complement(5288..5833)
                     /gene="cobU"
                     /locus_tag="ACHKTW_004750"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HFO6465633.1"
                     /translation="MMILVTGGARSGKSRHAEALIGDSSQVLYIATSQILDDEMAARI
                     EHHRQGRPAHWRTVERWQHLDELIHADINPHETVLLECVTTMVTNLLFDYGGDKDPGE
                     WDYQAMEQAINAEIQSLIAACQRCPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQ
                     QLAAAANEVWLVVSGIGVKIK"
     gene            complement(5967..6326)
                     /locus_tag="ACHKTW_004751"
     CDS             complement(5967..6326)
                     /locus_tag="ACHKTW_004751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001516590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HFO6465634.1"
                     /translation="MHCRGCGFIGEEWQQDFQPLDGGPVQSLSDAYSDYSVLLLIAAE
                     SEISVPEWFPHSEGTTVILIAEHNARLLTGPAGGAVALIHYVNEICHSRETAVNDAFC
                     RTLTRGSLAIPQCHLLS"
     gene            6305..6601
                     /locus_tag="ACHKTW_004752"
                     /pseudo
     CDS             6305..6601
                     /locus_tag="ACHKTW_004752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585847.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006576 - biogenic amine metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EutP/PduV family microcompartment system
                     protein"
     gene            complement(6702..7031)
                     /gene="tmaR"
                     /locus_tag="ACHKTW_004753"
     CDS             complement(6702..7031)
                     /gene="tmaR"
                     /locus_tag="ACHKTW_004753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HFO6465635.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(7203..>7706)
                     /locus_tag="ACHKTW_004754"
     CDS             complement(7203..>7706)
                     /locus_tag="ACHKTW_004754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6465636.1"
                     /translation="QSAFSPNLLERPRLESHLQKLLTDAVKMRGLIAPASKESRIPKS
                     IYEGIQTINRNLVCMLELQINAYWATRPSHFVLLNAQKLRDTQHMMQQILLSLVHALY
                     EGNPQPVFANTEKLNDAVEELRQLLNNHHDLKVVETPIYGYVWLNMETAHQLELLSNL
                     ICRALRK"
BASE COUNT         2018 a         2050 c         1715 g         1923 t
ORIGIN      
        1 ctgaaaagcc cgcttttata gcgggctttt tgctatatct gataatcaat ttcctcttca
       61 ctgttttcca tcacctgccg cttgatatcc tcaactgaca gtcctgcatt acaaagttcc
      121 agaaagcgcc agacatagtt acgctgaagt tgtcctcgct tcagtcccaa ccagacagta
      181 ttagcatcga aaagatgccg cgtatccaga cggattaaat tctcttcctc ttgttcgccg
      241 ctggattgtt cggcaactaa tccgatccca agcccaagag caacataggt tttaatgaca
      301 tcagaatcct gcgcacttaa tacaatatct gccagcaaac ctttgcgggc aaatgcgtca
      361 tcaatacgtg agcgccccgt aatcccccgt cggtaagtga ttaacggcca cttcgctatt
      421 gattccagcg tcaatggtgt aatttgcgtt aagggatgat cgagtggaac aagcaaacta
      481 tggtgccaac gaaaccacgg gaaggcgacg agctgcgggt cattactcaa acgctcgctg
      541 gcgataccaa tatcagcttc gccattttgc aacaacgtcg caatttcctg tggcgtcccc
      601 tggattagct cgagccgaac ctccgggaaa agttcgcgaa aagctttaat gacctctggc
      661 aagctataac gtgcctgagt atgcgtcgtt gcaatagtga gaacgccaga cgtatcgttg
      721 gtaaacaggt ctgcaagccg acgaacatta ctggcttcat tcagaatacg ttctgcaatg
      781 accagtaatg ctttgcccgg ttcagtcatg cccagcagtc gcttacctcg tcgaacaaat
      841 atttcgatgc caagttcatc ctccagttcc cgaatatgac ggctgacgcc tgactgtgag
      901 gtaaaaagca tattcgcaac ctctgtcagg ttgtaatcct gacgtgcagc ctcgcggatt
      961 atctttagtt gttggaaatt cacggtaaac tccgggcagt tcagatttcc cgttattgtt
     1021 aaagtctaat gcccggcata acaaataata aaaacccgca tcttattcca ttccgatata
     1081 acacttagct caccaattgc cactgccttt tttccatcac tggagaacta atcactgaca
     1141 ttaacaactc tttcactgcc tgtgcctgtg gcgataagtt cgctctggcg ggtaaattta
     1201 atgacaaaga gagactcatg gaaggagtgg taatgcgtga catccaccca tttactgcgc
     1261 cacataacga acgcgcggcc gattcgggta atactgcaac gcccatgccg ctggcaatcg
     1321 ctgcggtaag cgtggcaata gactcaattt caccaataac ttttgccgtg agtcgccgta
     1381 gggaaaaagc ctcatcaaca cgaagtctaa tagcactgta atcactgggg agaaagaggt
     1441 tcatttgcgc aatagcattc acatcaacgc tttgccccgg acaatcttga gttcctacca
     1501 ggaaaagatc ttctttcagc aaagcctgac tggatacccc agccacaggg gaatgctcat
     1561 aaatcaccgc catatcgagt tggtgattta tcagtttttc gttaagcact gcaccgctat
     1621 tttcatgaag atagataacg atctccggga attcagcgcg aaccgcctgt aataagggca
     1681 tggtgatgga tgacgcagcg gttcctggcg caaagccaat cgagacttgc cccgataatg
     1741 cctgaccaac gttatgcacc gccagttggg cctgctcaca ctgacgtaaa atggcccgcg
     1801 catgggtata gagaattttt ccggcgtctg ttggcgtaac gccccgtttt ttgcgaatca
     1861 aaagttgttg atttaactca ccttccagtg tggcaacctg ctggctgagc gctggttgtg
     1921 caatatgcaa tacttcagca gcctgggtca ggctaccaat atctacaatt tttacgaagt
     1981 atttcaggcg tctgaagttc atgttgcctc cggtttttaa gaatcggccc aagtgccgcc
     2041 attacttaca accagactgc aagatgcttg ccagttttat tttggcgttg atgtacaagc
     2101 taaccaactg tcaaataaga gattatgata gattcgtcat ttgctccttt aatcagctgt
     2161 cgcgttcccc tgccctataa aaggagggta tgcaccacga tgattcatta cccaataaga
     2221 ttgaaagctc accactttgt tgaaattgac agcaaacaaa caaaaaaatg catttcaccc
     2281 tttgacatca ccatgcactg ccattaatat gcgccccgtt cacacgattc ctctgtagtt
     2341 cagtcggtag aacggcggac tgttaatccg tatgtcactg gttcgagtcc agtcagagga
     2401 gccaaattca aaaaagcctg ctttctagca ggctttttgc tttctaatta ccaacgctct
     2461 taaaacatct gtcttgaacc agaactaatt tgcacaggca ttcccgatcg acgttgcaac
     2521 gcagcatttg cgcgatttac atcaacttct tgcccgctga taaacgcccg caaagatggg
     2581 gttaccggca atggcacttt tcggtcagac tcatattctg cacgattgcg cgacaacggc
     2641 tcatgaactt ccagccagtt cgagccatct ggttcagtgg tgtattttac tggctggtcg
     2701 ataatttgca cacgcgtccc aacaggaaca ttatcaaaca gatatttgat atcgtcattg
     2761 cgcagacgaa tacagccctg acttacccgg agcccaatac caaaattggc attggtacca
     2821 tggatggcat acaacctgcc aatataaatc gcgtacagcc ccatgggatt atcggggccc
     2881 gcaggaacaa atgcgggcaa actctcccct cgtttcgcat attcgcgccg agtgttcggc
     2941 gttggcgtcc aggttggagc ttcttgttta cgttcaacgg tagtcaccca gttacgcggg
     3001 gtttctcgcc cagcctggcc gataccaata ggaaagactt ccacagtatt actgtctggt
     3061 gggtagtaat aaagacgcat ctcagcgacg ttaaccacaa tccctttacg aacagtggcg
     3121 ggcaaaatca gttgctgcgg aatggtgagt tgcgagccag acttcggcaa aaaaacatca
     3181 gcgcccgggt tcgcttccag catgttactt aacccttgcc cgtattgtgc ggcaaaagtc
     3241 tccagcggct gggtattgtg atcaggaaca gttacagtaa acgactgccc cactaaacgg
     3301 ctaccctctg gaggtaatgg ataagttacc gccaggctag tatggctggc aaaaagtaga
     3361 gcaaatgagc aaaaaatatt tacacgacgc atcatgtccc tttcctaagt cgcgaaagct
     3421 atccgttaat tatagctttt atcagacttt tcgtttttaa ctgttcaaat cagaagtcgt
     3481 attccccggt agaacaatat tactggcagc aagttcgccc atgttgttat atatcgcaca
     3541 ggcagcttcg atgatgggca tcgccagagc tgcgccactc ccctcaccta aacgcatctc
     3601 catattgaga taaggctcca gccccaaatg cgacagcgca atacgcgccc ctttttctgc
     3661 cgacaggtga gaaggaatga gatacggttt gattgcagga gacatctggc aggctgcgag
     3721 cgcggcagca taagaaagaa atccatccag caacacgggt aaaccgcagg aagcagcgcc
     3781 taacatcacg ccagccattc cgaccaaatc aaatccaccc acttttgcca ggacatcaac
     3841 gccatcctgc ggatttggtt gattcaacgt aatcgcccgg cgcacaacat caattttatt
     3901 agccagttta tctgtcggca ggtttgcgcc aatcccaacc acttcttcag gagcccggcc
     3961 agtgattgtg ctgactattg ccgctgccgg tgtcgtgttt gccatcccca gttcacctac
     4021 accaaacagc gtaacaccgt tttttgccag ctcccgcgta taacatatga cgtccaaaag
     4081 caacttttct gcctgatggc gactcattgc cggagctgaa gcaatattgc cactacctcg
     4141 agcaacacgc atgttgataa gcccggggat aggctcagca gtatcaatac caacatcaat
     4201 tacgtagacg ttagcgcccg cttgtgctgc cagcacacac acgccggttg ttccgcgggt
     4261 catattttca gcctgaatgg ctgtcacttc ttttggggaa atagcgaccc cttcctccca
     4321 gacgccgtga tcggcacaca tgaccagtac cgcttttttg cccacatgcg gaacgccatt
     4381 caaccccagc attcctgcca gttgtatggc aagcgcctcc agccttccca ggctaccaac
     4441 aggtttgagt aacccgtcga tatgccgttg tgcacgcgac atagcggcag gatcgatagc
     4501 agggatcgta ttcagtaaat cggcaagtgt ttgcatgtca cgtctcttta tagcagtgcc
     4561 agcaggaaga ccagttcacc aagctctata gcagctccca gcgtatcgcc ggtttgcccg
     4621 cccagcgtac gtttgagaag ctgaccgagg atgaaaatcg ccaccatcgt caccaccata
     4681 gcagccacac catgcatacc cggcaataat acggcagcaa aaatagcggc taagccgagt
     4741 gtgacgcagg tttgtcgccc atcaattttg ccaatgaaca cattgccaag cccttcttcc
     4801 cgggcataac gatgacgata catcaataat gcggcagtcc cgcggctgac cgcacatgct
     4861 gccgccagcg atgcaaggat cggttcgcca cgcagagcca actcgcttaa caccagaatc
     4921 tttgccagta caacaaaaat caatgccaga ccgccgtggg tgcctaaacg gctatcacgc
     4981 atgatttcca acatgcgatc gcggctacgt gcagaaaata cgccatcaca ggtatcggcg
     5041 aggccgtcca ggtggaatcc cccggtcatc agcgccagca caagtacgct aaacagtgcc
     5101 gccagtggta cgccacacca tgcctgcagc accatgaaga ccagcccgct aatcgcgcca
     5161 agcaataatc caattaaagg aaaagtaata ataccgcgag aataatgctc gaaatccagt
     5221 ccctgggacc agcgacgcgg tacaggcaga cgcgtaataa aagagagcat cgcccaaaat
     5281 aatttactca tttgattttt actcctatac ccgaaaccac cagccatact tcatttgccg
     5341 cagcggccag ctgctgattt acccgcccgg caatatcacg aaaatgtcgt gccagacgac
     5401 tctccggcac aatccccatt cccacttcgt tagtcactaa tacaaccttt gcgggacaac
     5461 gttggcaggc agcaatcaac gactgaatct cagcattaat cgcctgttcc atcgcctgat
     5521 aatcccattc accagggtct ttatcgccgc cataatcaaa caacagatta gtcaccattg
     5581 tggtaacgca ttccagcaat acagtttcat gcgggttaat gtctgcatga attaattcat
     5641 caagatgttg ccaacgctcc accgtgcgcc agtgcgccgg gcggccttgc cgatgatgtt
     5701 ctatccgtgc agccatctca tcatcaagga tttgtgaggt ggcgatatac agaacctgtg
     5761 aagagtcccc aataagcgcc tctgcgtggc gactcttccc gctccgtgcg ccccccgtca
     5821 ccaaaatcat cataccgact cctgattcaa ttgtatgatg gtattcattg aacagaacaa
     5881 tggatccccc accgttgctg accgcaaaac atttacgcga ttaatactgc gcgtaatata
     5941 attaaaaatc cagcattctc aatccattac gaaagaagat ggcattgcgg aatagccaga
     6001 cttcctcgtg ttaacgtccg acaaaatgcg tcattgacag cggtttctct ggaatgacag
     6061 atttcattca cataatggat taatgctaca gccccaccag ccggaccagt cagcaaacgg
     6121 gcattgtgtt ctgcaatcaa aattaccgtc gttccctcag aatgagggaa ccattctggt
     6181 acggatatct cgctttcagc tgctatcaat agcagcactg aataatcgct atacgcatct
     6241 gataacgatt gcacaggccc accatcaaga ggctggaaat cttgctgcca ctcctcaccg
     6301 ataaaaccgc agcctctaca gtgcattgtt ggtcagtgcc tgcgaagcag acattatcgc
     6361 gctggtgctt aatgcggacg caccgtggtc acctttttcg ccaggcttta cctcgtcaat
     6421 gaatcgcccg gtcattggag tagtcaccaa agcagattta gccagcatgg aacaaatatc
     6481 tttggtgaaa tgttggttac gtgaagctgg agcgcacaac gtgttagtaa ccagtgcggt
     6541 taataacaat ggagttgcag aactttttgc cttgctgcat acagaagaag gctgtcgcta
     6601 atttcgacag gttctcagat gagaatgatg acggtgatac acagaaaaga atcaatgagc
     6661 aatgagggtt gccaggcaac cctcattgaa taaaacagga attacttcgc ttcgccgttt
     6721 ttcatttcgc ccatagcttt cagctttttg gagatatcgc ggcgttcttt ggagagctcg
     6781 gcatttttga tgatgtaatc gtcaacgcga tcttcatagt cacctttcat gctggcgatg
     6841 atgccctgga ttgcttcaac gctcatcccc ggcttgatgt aatcgctcag gttgtccagc
     6901 agcaggacgc gcttctggtt gtcacggatc tttttctcaa cgtcctgaat ttcacgttgc
     6961 agtttgttct tacgacggaa cagacgaaca aattccagta cgtcctggaa tgaaggcttg
     7021 gtagtttcca tttttatacc cctgataatg tgagagtcgg attcgtttaa gcaaccgcta
     7081 ttcgttaggg ccaacattac tcatagctgc cgcagatgac aatgctttta tcccttttca
     7141 ctatcatacc ctttatcctt gctgaatcga agcagcagca agatgattct gaagttcagg
     7201 aattatttgc gcaaggcccg gcaaatcaga ttcgatagca actcaagctg atgtgccgtt
     7261 tccatgttca gccacacata accatagatt ggtgtttcca caaccttcag gtcatggtgg
     7321 ttattgagca actgacgcag ctcttccaca gcatcgttca atttttccgt attggcaaaa
     7381 accggctgcg gattaccttc gtacagcgca tgaacaaggc tcagcagtat ttgctgcatc
     7441 atgtgctggg tatcacgaag tttttgcgcg ttcaataaca cgaaatggct ggggcgcgtg
     7501 gcccagtacg cattgatttg caactccagc atacaaacca gattgcgatt aatggtctga
     7561 ataccttcat atatcgattt tggaatacga ctttctttgc tggcgggcgc aatcagtcca
     7621 cgcattttca cggcatcggt caggagtttt tgtagatggc tttccagacg tgggcgttcg
     7681 agtaagttcg gtgagaatgc agattg
//
LOCUS       DAZFQD010000194         7683 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.194, whole genome shotgun sequence.
ACCESSION   DAZFQD010000194 DAZFQD010000000
VERSION     DAZFQD010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7683
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.194"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1034
                     /gene="cka"
                     /locus_tag="ACHKTW_004755"
     CDS             <1..1034
                     /gene="cka"
                     /locus_tag="ACHKTW_004755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_159420795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin K"
                     /protein_id="HFO6465637.1"
                     /translation="KKSNQLDADISKLNSYKNTLQSKIGDVNKQKEAEEKARENAEVA
                     EHETLNEEKQAVVEAEKRLAEAKAELAKAESDVQSKQATVSRVAGELENAQKSVDVKV
                     TGFPGWRDVQKKLQRQLEAKQAEYSAVENELKNAVSFRDGKAAEVKEAEQKLKEAQDA
                     LEKSQIKDAVDTMVGFYQYITEQYGEKYAKIAQDLAEKSKGKKIQGVDEALAAFEKYK
                     NVLDKKFSKVDRDAIFNALESVNYDELSKNLTKISKSLKITSRVSFLYDVGTDFKNAI
                     ETGNWRPLFVTLEKSAVDVGVAKIVALMFSFIVGVPLGFWGIAIVTGIVSSYIGDDEL
                     NKLNELLGI"
     gene            complement(1031..1321)
                     /gene="cki"
                     /locus_tag="ACHKTW_004756"
     CDS             complement(1031..1321)
                     /gene="cki"
                     /locus_tag="ACHKTW_004756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011264161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin K immunity protein"
                     /protein_id="HFO6465638.1"
                     /translation="MYLKYYLHNLPESLIPWILILIFNDNDNTPLLFVFISSIHVLLY
                     PYSKLTISRYIKENTKLKKEPWYLCKLSALFYLLMAIPVGLPSFIYYTLKRN"
     gene            1345..1476
                     /locus_tag="ACHKTW_004757"
     CDS             1345..1476
                     /locus_tag="ACHKTW_004757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_072023513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="HFO6465639.1"
                     /translation="MKIVIFLITILFLSGCQANYIRDVQGGTVAPSSSSELTGIAVQ"
     gene            complement(1675..1818)
                     /locus_tag="ACHKTW_004758"
     CDS             complement(1675..1818)
                     /locus_tag="ACHKTW_004758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFO6465640.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDLTPLAAAIGNWIVDLDT
                     QNS"
     gene            complement(2483..2680)
                     /locus_tag="ACHKTW_004759"
     CDS             complement(2483..2680)
                     /locus_tag="ACHKTW_004759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HFO6465641.1"
                     /translation="MNKQQQTALNMARFIKSQSLTLLEKLDALDADEQAAMCERLHEL
                     AEELQNSIQTHFEAQYGIEQP"
     gene            3301..3495
                     /locus_tag="ACHKTW_004760"
     CDS             3301..3495
                     /locus_tag="ACHKTW_004760"
                     /inference="COORDINATES: protein motif:HMM:NF033650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HFO6465642.1"
                     /translation="MNQDKAIYLENKYFSPANEAVRLEREGDYIGAWKKWNDALHYAK
                     GENIGWCIARAEYCKKTGRF"
     gene            complement(5726..6148)
                     /locus_tag="ACHKTW_004761"
     CDS             complement(5726..6148)
                     /locus_tag="ACHKTW_004761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="HFO6465643.1"
                     /translation="MRSAAARLLLIPLITATIALTGCTPKTSLERHTRHYVYASDDGF
                     DPNFYTQKSDTIRMMLPFFQQFRDMGMKDKAAGVSAETAQQRVKEFHSEKFFHSLRST
                     TTFAGRKYTNSDMPSPKKMKLMADTISAVYLDGYEGRQ"
     gene            6270..6548
                     /locus_tag="ACHKTW_004762"
     CDS             6270..6548
                     /locus_tag="ACHKTW_004762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6465644.1"
                     /translation="MKKCVPVLLFSVLSASVASHADSNVFQQKAEKTKNGIVADVNAR
                     QKMFSNRLKIQLQINELRAELAGTRDAQKREDLRKKLESLERQKKSLR"
     gene            complement(6540..6830)
                     /locus_tag="ACHKTW_004763"
     CDS             complement(6540..6830)
                     /locus_tag="ACHKTW_004763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001672407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6465645.1"
                     /translation="MSKSPRHKLNKTDKRLLDTLVAAGYEYDKARDLIQKQVYTLTLA
                     DQRHVVSEISNGVNPTQAYSAVYQARRIRLARKYLNGKKVMEETGENTPPSA"
     gene            7071..>7683
                     /gene="cka"
                     /locus_tag="ACHKTW_004764"
     CDS             7071..>7683
                     /gene="cka"
                     /locus_tag="ACHKTW_004764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_159420795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin K"
                     /protein_id="HFO6465646.1"
                     /translation="MAKELSGYGPTAGESMGGTGANLNQQGGNNNSNSGVHWGGGSGH
                     GNNGGQGNSNSSGSTSTVMKTGESYLTPWGDVVINNDGLPVMNGIVMTEENSTLVDNP
                     FGGVSRVLNSLISDMPSLFAESSGNNNNNTASVNTAPTNAQVSDMDKSSKVVGNVINE
                     KQKQKNKIATQISEKQKKIEEMKKVFKHHSYHGITDLERDVDEL"
BASE COUNT         2110 a         1582 c         1866 g         2125 t
ORIGIN      
        1 aaaaaaaatc aaatcagctt gatgcagata ttagcaaact taattcttat aaaaatacat
       61 tgcaaagtaa aattggtgat gtgaataaac agaaggaagc tgaagaaaag gctcgtgaaa
      121 atgcagaggt cgctgaacat gaaactctta atgaagagaa acaggcggtg gtggaagcag
      181 agaagcgttt agctgaggcg aaagctgaac tggcgaaggc tgaaagtgat gtacagagta
      241 agcaagctac tgtttccaga gttgcaggag agcttgaaaa cgctcagaag agtgttgacg
      301 tgaaggtcac cggatttccg ggatggcgtg acgttcagaa aaaactacag cgtcagcttg
      361 aagctaagca ggctgagtac tccgcagttg aaaatgaact taagaatgct gttagtttca
      421 gggatggtaa ggccgctgag gttaaagagg cagagcagaa attaaaagaa gctcaggatg
      481 ctcttgaaaa gagccagata aaagatgctg ttgatactat ggttggattt tatcaatata
      541 taaccgaaca atatggagaa aaatacgcaa aaattgctca ggatttagcg gaaaagtcta
      601 aaggaaagaa aattcaaggt gttgatgaag ctctggcagc atttgaaaag tataaaaatg
      661 tactggataa gaaattcagt aaggttgatc gggatgctat ttttaatgcg ttagagtctg
      721 ttaattatga tgagttatct aagaatttaa cgaagatatc aaagtcgctt aaaatcacta
      781 gtcgagtatc ttttctatat gatgttggta ctgattttaa aaatgcaata gaaactggta
      841 actggcgacc tttgtttgtc acgctggaga aaagtgcggt agatgtaggt gtggcaaaaa
      901 ttgtggcatt aatgtttagt tttattgttg gtgtgcctct tggtttctgg gggattgcaa
      961 ttgtcacagg tattgtttct tcttacatag gggatgatga gttgaacaaa cttaatgagt
     1021 tgttagggat ttaatttctc tttagagtgt aatatatgaa acttggcaat cctactggga
     1081 ttgccattaa taaataaaac aatgcagata acttgcataa gtaccagggc tcttttttta
     1141 actttgtatt ttctttgata tatctagata tggttaattt agagtatgga tatagcaata
     1201 catgtattga tgatataaag acaaataaca aaggagtgtt atcattgtcg ttaaatatta
     1261 aaataagaat ccatggtata agtgattcag gtaaattatg taggtagtat tttagataca
     1321 taatttttct tttgttggag gtgtatgaaa atagtaattt ttctaattac aatattattc
     1381 ttgtcaggat gtcaagcaaa ctatattcgt gatgtacaag gaggaaccgt tgcaccatct
     1441 tcgtcttctg aactgacggg gatcgcggtt cagtagaaaa gatcaaagga tcttcttgag
     1501 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctgccaacgg
     1561 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggctttagtg
     1621 gagcacagat accaaatgct gtccttccag tgtagccgct ataaggcttc aacttcaaga
     1681 gttctgcgta tctaaatcca ctatccagtt accaatggct gctgccagtg gcgttaagtc
     1741 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     1801 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     1861 ccaacagcgt gagctatgag aaagcgccac gtttcccgaa gggagaaagg cggacaggta
     1921 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccgg ggggaaacgc
     1981 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     2041 atgctcgtca ggggggcgga gcctatggaa aaacgccagt ggcgcggcct tttccgggtt
     2101 ctcgtttttt gcgcacatgt ttctttcggc gttatcccct gattctgtgg ataacggtat
     2161 caccgtcttt gagtgaggtg actccgctcg ccgcagccga acgaccgagc gtagcgagtc
     2221 agtgagcgag gaagcggaag tgtaccggaa gctgcatttt ctccttacgc atctatgcgg
     2281 catttcacac ccggcatggt gcacttttca tacaatccgc actgatgcca catgattaag
     2341 ccagtataca ctccgctacc gctacgtgac tgggtcatgg ctgcgccccg acgcccgcta
     2401 aagccccctg acgtgccctg acgggctttg tctgctggcg aaaacactca cggacaagcc
     2461 gcgaccactt tcgccagcgc ctttatggtt gttctatgcc gtactgagct tcaaagtgag
     2521 tctgtatgct gttctggagt tcttccgcca gttcgtgcag tcgttcgcac atggcggcct
     2581 gttcgtctgc atccagtgca tccagttttt cgagcaacgt caggctctgg cttttgataa
     2641 atcgcgccat gttgagcgcg gtctgctgct gtttgttcac gttctcccgt ctcctttact
     2701 ggtgtttacg tccggcagtg cgtcacctgg tgtgatgacc cggtgacgtc actgaaaatt
     2761 atcacacaag cccagtcgcg cgtgtgctgc gcgacattaa caccccgtga cgcttatttt
     2821 ttgctgaata tgtgcgcgat tttctgtctg cgttgtgtgc agttgttgtc ataaacacga
     2881 atgttccggc tgttcctgat gtgcgtcact ttgacgtttt gacaaagtga cgcacgggag
     2941 gtgcttgcgc aaacccgcga ataacggcgt ttaccgcgtg attttttaac atctgacaaa
     3001 gtgacccggc gtgatggcca gttctggcca gagacgttgg cggggtgtcg gggcgcagcc
     3061 atgactaggc ggcatttgtc tgattgcgtg tgcgcggtcc gacaaataca catcctgtcc
     3121 cgtttttcgc acggttttta ttgattaccg gacagatgaa cccggattgc catgttgcga
     3181 aaatcacgtt aaaacgatta cgagcgtgct gttgggctga tatatcaaca gtcatgccaa
     3241 tatgtattaa cccgatgggc tcgctctttt ttaattcaaa taaaaatata aattacgaat
     3301 atgaatcagg acaaagcaat ttatttagaa aataaatatt tttcacccgc aaacgaagct
     3361 gtcagactgg agcgtgaagg tgattacatt ggagcatgga aaaaatggaa tgatgcgctt
     3421 cattatgcca aaggagaaaa tataggttgg tgtatagcca gagctgaata ctgtaaaaag
     3481 acaggccgct tttaattctg gcggggtttc aaaggggcgt ccactttggc gcgtcgacgt
     3541 atcaaaatgc gccagcattt tgtatacagt cggtcacgtg cctccagaat ttaccctcaa
     3601 aacgcccctg caactgatac acaacaaaag tataactaat atgtagttat tatataacta
     3661 atgcataata aacagctcac caacggatcg catgtagtag acgctacata aaaaatcctg
     3721 aaatggagga tttgtcataa agagattcct gtttttttag cgttctgatg caggacaggg
     3781 caaacaccgc aaaaatgatg aaaaactgat taaccatatt gtctgtattc tgaatgaatt
     3841 acgaagcata gcgataaatt aaaaaatccc cctggattac agggggaagc catcagagtt
     3901 tgagtaatga gaaatattat tttggagaga gcaccacaaa tgcgctcttt tccaatctgc
     3961 cgtaaccatc ggcaaaagac aatcaggaca ggcgaaaaaa tccccctata tggcaagggg
     4021 gtgtcaatga ggacatggca attgtgagtt ttgtgtgtgt tttatgtgtt gcagtttgca
     4081 acgtcagaaa tttgccgtag ttcgtcatga gagacaacaa aaaaatcccc cgtgactggt
     4141 ggaggccagg gcaagggggg tagcaaatgc ctgaataatt ttcgtagttt tgatataccc
     4201 atcgttgcaa ccgcaacggg atgaatttgc cgcagttgtg cgcgaaaaac aatcctgatt
     4261 ccgggacagg atgtgtaatc gtataaccgc gcacgcacgg ctatgcgatt accacctggt
     4321 cagggctttg ccccgacagc ctcgacaccg ttcccgcgat acgatagaac atcagggcgc
     4381 gtttccgggt aaacgcatgt taatgaccgg gaattcattg atcagggacc ggagacgcgc
     4441 tacaaaactc ctgcgcgttt ctggcatcaa ctggccatgg ggatggtttg gcagtagcag
     4501 caagcgccag aaacgcgctc aaaacgtctc taacagcctg aacccaccaa aacataatga
     4561 aacattgcca gatgcaaaaa agtgcgttag aaagcgtctg agcgcgtttc cttgccacct
     4621 gaacgtcatc tctgggattc gagctcactc catcaaataa caaataaact tcaaagtctg
     4681 ataatgggtt ccccctgtaa tccaggggat ttttttcgct cactcattac cgcacaactc
     4741 gacatcacgc cggagacacg ctaaaacacc ctgcgcgttt ctggtgcaaa gagtagttcg
     4801 tagggatgat tacagtcagg tatgtaccag aatcgcgccc tgaacacggc tgaacgcctc
     4861 aaagcgaaac cattgcctca tgtaaaaaat cgcgttacaa agcctctgag cgcgtttctg
     4921 gcgtcttttt tgttgctgtg tcgctatact cgttgtcaga ttcgcgctca atcatcactg
     4981 attaatctgc gcttatcctt cgaccgatca cgctggtatt ttttgacaac cggcgcccat
     5041 gaaaacgcca gacgggaacc gtcatccgca ccctctgaca tgctggggat taaaaatccc
     5101 tgtgccgcac agccggcgtt gcataaaatt ccgctctgcg atgagtattg tttaatcaaa
     5161 aatccccgca gtttctccag aaaaacgcga gtctgttctc atgccccgga tgcccacccc
     5221 ccacgcaaaa ccaacttttt tctgatttgc tttttaaatc atgttgttat ggtttgaaaa
     5281 atatattttc ttattcagat ttatacaaaa gcgagttgtc ggcgactact ggatgattcg
     5341 gcaacgagtc gtcggtgatt tatcgatgac tcaacacact gtctctctgg tgttcagaaa
     5401 aagcgagtcg ccagagagtc gcgaggaaaa atactggatg actcgctgat gactcccggg
     5461 gtgaatcgct cacgactcgt ctgtaactac tgtctgattt cttctcatca gggctgaaaa
     5521 cgtccgaaat gcgccttatg ggcatcaggc gcgcccaaac cgatcgccat tcagtttcat
     5581 gcatagtcat gcagtgccat gaaaacaatc ctgacgtatt tttccggttt acccggggga
     5641 aaacatctct ttttgcggtt tttgcgccag aatcgcgctg agcgcgtttc agcggtgcgt
     5701 acaataaggg attatggtaa atcccttact gtctgccctc gtatccatcg agataaaccg
     5761 cagaaatggt gtctgccatt agtttcattt ttttcggcga aggcatatcg ctgtttgtgt
     5821 attttctgcc agcaaaggtt gttgtgctcc ggagtgagtg aaaaaatttt tctgagtgga
     5881 attcttttac acgttgctgt gccgtttctg ctgatactcc ggctgctttg tctttcatcc
     5941 ccatatcccg gaactgctga aagaaaggga gcatcatacg tatggtgtct gatttctggg
     6001 tgtagaagtt aggatcaaat ccatcatctg aagcataaac ataatgccgg gtatgtcgtt
     6061 ccaggctggt ctttggtgtg catcctgtaa gagctattgt tgctgttatc agaggtatca
     6121 gaagcagcct ggcagcggca gatctcataa acatctcctt gcgtttttaa tcttgatact
     6181 cagggagtta tatctccgaa gagtatctct tatcatacgt cggtttactc ccaatgtgat
     6241 ttgatggtca atgagtcagg aataatcccg tgaaaaaatg tgtgccagtc ctgcttttta
     6301 gtgttctttc tgcttcggtt gccagtcatg cggacagcaa tgtttttcaa caaaaggccg
     6361 agaagacaaa aaacggcatt gttgcggatg ttaatgccag acagaaaatg ttcagtaaca
     6421 gactgaagat acaacttcag atcaatgagt tgagagcaga gctggctggc accagagatg
     6481 cccaaaaaag agaggatttg cgtaaaaaac tggagtctct ggagcggcaa aagaaatcct
     6541 tacgctgatg ggggcgtatt ttccccggtt tcttccataa cctttttacc gttcagatat
     6601 ttacgggcga ggcgaatgcg tcttgcctgg tataccgctg agtaagcctg ggtgggattc
     6661 acaccattac tgatttcact gaccacatga cgctgatcag ccagtgtcag cgtgtaaacc
     6721 tgtttctgga tgaggtcacg ggctttgtca tactcatatc cggcagcaac aagggtgtcg
     6781 agaagtcgtt tgtctgtttt gttcagttta tggcggggtg atttgctcac atcgttctcc
     6841 gttttttcat gatccaacag ttttatatct catcctgatt tgctccggtg ttctgtttcg
     6901 acgttctcaa tgcctgcctc cccatagtgt gatccttatt gcaaaaatga aaatccatgc
     6961 tcttgacacg gacaatgctg agtagtaggt tttcactgta cataaaacca gtggttatat
     7021 atacagtata ttgtttttaa tttattgttt taattatcaa agagaaattt atggctaaag
     7081 aactaagtgg atatggccca actgctggtg agtctatggg gggaacaggg gcgaatctga
     7141 atcagcaagg tggaaataat aatagtaata gtggggttca ctggggtggt ggctctgggc
     7201 atggtaataa tggtggtcag ggaaattcaa attcatctgg ttctacatca actgtgatga
     7261 aaaccggtga gtcatatctg actccgtggg gtgatgttgt cattaataat gatggccttc
     7321 cggtgatgaa tggtattgtt atgacagaag agaattcaac tctggttgat aatccatttg
     7381 gaggggtctc cagagttctt aattctttaa ttagtgatat gccatcactt tttgcagaaa
     7441 gctcaggaaa taacaacaat aacactgctt cagttaatac tgcaccaacg aatgctcagg
     7501 tcagtgatat ggataagagt tcgaaggtgg tgggtaatgt aattaatgaa aaacaaaagc
     7561 agaagaataa aattgctaca cagattagtg aaaaacagaa gaaaatcgag gagatgaaaa
     7621 aagtattcaa gcatcattct tatcatggta tcactgatct tgagagggat gttgatgagt
     7681 tgc
//
LOCUS       DAZFQD010000195         7674 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.195, whole genome shotgun sequence.
ACCESSION   DAZFQD010000195 DAZFQD010000000
VERSION     DAZFQD010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7674
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.195"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(29..105)
                     /locus_tag="ACHKTW_004765"
     tRNA            complement(29..105)
                     /locus_tag="ACHKTW_004765"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(69..71),aa:Arg,seq:acg)
     gene            complement(303..379)
                     /locus_tag="ACHKTW_004766"
     tRNA            complement(303..379)
                     /locus_tag="ACHKTW_004766"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(343..345),aa:Arg,seq:acg)
     gene            complement(383..475)
                     /locus_tag="ACHKTW_004767"
     tRNA            complement(383..475)
                     /locus_tag="ACHKTW_004767"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(439..441),aa:Ser,seq:gct)
     gene            complement(791..976)
                     /gene="csrA"
                     /locus_tag="ACHKTW_004768"
     CDS             complement(791..976)
                     /gene="csrA"
                     /locus_tag="ACHKTW_004768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HFO6465647.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(1211..3841)
                     /gene="alaS"
                     /locus_tag="ACHKTW_004769"
     CDS             complement(1211..3841)
                     /gene="alaS"
                     /locus_tag="ACHKTW_004769"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFO6465648.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADVDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMDLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(3969..4469)
                     /gene="recX"
                     /locus_tag="ACHKTW_004770"
     CDS             complement(3969..4469)
                     /gene="recX"
                     /locus_tag="ACHKTW_004770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFO6465649.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(4712..5773)
                     /gene="recA"
                     /locus_tag="ACHKTW_004771"
     CDS             complement(4712..5773)
                     /gene="recA"
                     /locus_tag="ACHKTW_004771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFO6465650.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(5853..6350)
                     /gene="pncC"
                     /locus_tag="ACHKTW_004772"
     CDS             complement(5853..6350)
                     /gene="pncC"
                     /locus_tag="ACHKTW_004772"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HFO6465651.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMVIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(6495..7580)
                     /gene="mltB"
                     /locus_tag="ACHKTW_004773"
     CDS             complement(6495..7580)
                     /gene="mltB"
                     /locus_tag="ACHKTW_004773"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HFO6465652.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDKMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAVMAN
                     GQAPGLPNGFKTRYSISQLATAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
BASE COUNT         1786 a         2153 c         1859 g         1876 t
ORIGIN      
        1 cgctactaaa gccatatcaa gtaagagatg gtgcatccgg gaggattcga acctccgacc
       61 gctcggttcg tagccgagta ctctatccag ctgagctacg gatgcatcgg gaaacttact
      121 ttactgcaga ttttttgata ccgctactaa agccgtatca agtaagagat ggtgcatccg
      181 ggaggattac tcgccttgcg gctcgccctt cgggccgttg ctaaagcaac gttatcctcc
      241 ctggtgcctg cggttatacc gccacctctt ccataccttc atcgctgaaa gtacgtagaa
      301 tatggtgcat ccgggaggat tcgaacctcc gaccgctcgg ttcgtagccg agtactctat
      361 ccagctgagc tacggatgca aatggcggtg aggcggggat tcgaaccccg gatgcagctt
      421 ttgaccgcat actcccttag caggggagcg ccttcagcct ctcggccacc tcaccacacg
      481 cctcttacga gtgcttcgaa gaacttgttt atcgctcatc gtcgctgcgt ggcgcacata
      541 ttactttctg agacttataa gtcaaacaat ttttcctaca cttttatcgt ttgcacactt
      601 cacgttcaat tagtctgtaa aaacgtcaaa aagagtgttt tatcaacaga aaaatggagg
      661 tctgacagat agtagtaatg caaaaaaatg gagacttaag ttgaatgaac gggagtaaag
      721 cgaaaagact atagagtgaa ggagaaattt tgagggtgcg tctcaccgat aaagatgaga
      781 cgcggaaaga ttagtaactg gactgctggg atttttcagc ctggatacgc tggtagatct
      841 cttcacggtg aacagaaact tccttcgggg catttacgcc aatacgtacc tggttgccct
      901 ttacccctaa aactgtcacg gtgacctcat ccccaatcat gagggtctca ccaactcgac
      961 gagtcagaat cagcattctt tgctccttga aagattaaaa gagtcgggtc tctctgtatc
     1021 ccggcattat ccatcatata acgccaaaaa gtaagcaatg acaaacacat tacatgtaag
     1081 cagtcatggc attacattct gttaaaccta agtttagccg atatacacaa cttcaacctg
     1141 actttatcgt tgtcgatagc gttaatgcga atgccgtgaa gcgagtccac ggcattgcct
     1201 gacgcttata ttattgcaat ttcgcgctga cccagccttt cacactggct aacgctgcag
     1261 gtaaggccgc agcatccgta ccaccggctt gcgccatgtc aggacgtcca ccacccttgc
     1321 cgcccacctg ctgagcgacc ataccaatca gttcccctgc tttcacacga tctgtgacgt
     1381 ccttagatac gcctgcaatc agagaaacct taccttcggc taccgttgcc agcacgataa
     1441 ttgtcgaccc cagctgattt tttaaatcgt caaccatggt acgcaacatt ttcggctcaa
     1501 caccgctaag ctcgctaacc aacagcttaa caccattaac atcaattgcc ttactggaaa
     1561 gatttgcgct ctcctgtgcg gcagcttgtt ctttaagctg ttgtagttct ttttctaact
     1621 gacgcgtacg ttccagtact gagcgcactt tatccgccag attattgcta tcgcctttca
     1681 gcagatgcgc gacttcgctt aagcgatcgc tgtctgcatg aacggtggtg atagcgcctt
     1741 ctccggttac cgcttcgata cgacgaacgc ctgcagcagt acccgattca gagatgatgc
     1801 ggaacagacc aatatcacca gtgcggctgg cgtgagtacc gccacacagc tcggtggaga
     1861 aatcgcccat gctcagcacg cgtacacgct catcatactt ctcgccgaac agcgccatcg
     1921 cacctttcgc tttcgccgct tcgagatcca tgatgttggt ttcgatcggc aaattgcgac
     1981 gaatctgtgc gttcaccagg tcttcgaccg cacgaatctc ttccggtttc atcgcttcgt
     2041 tgtgtgagaa gtcgaagcgc agcaccttgt cgttaaccag tgaacctttc tgcgatacat
     2101 gagtccccag aacctggcgc agcgcagcgt gcatcaggtg cgttgcggag tgattcagac
     2161 gaatacgggc gcgacgagcc tcatcaacat cagcctgcac agcgtcgccc actttcagag
     2221 aacccgcagc aagtttaccg atgtgaccaa tcgcctggcc gtatttctgc gtatcttcca
     2281 ccgcgaagga gaagttagcg cctttcagtt cgcctttatc accaacctga ccgccggatt
     2341 ccgcatagaa tggtgtttga tccagcacga ccacagcttc ctggcctgca ttgatggcat
     2401 caaccgcttt accatcaaca aacagcgcgg tcactttgcc gttcagttcc agatggtcat
     2461 agcctttaaa ttcagatgca ctatcaacac ggatcattgc gttgtaatcg gcaccaaagc
     2521 cgctggcttc gcgcgcgcga cgacgctgct cttccattgc tgcttcaaaa ccagcttcgt
     2581 caactttgat gttgcgctca cgacaaacat cagccgtcag gtcaaccggg aagccatagg
     2641 tgtcgtacag acggaaagcc gtttcaccat ccagcgtatc accagaaagt tttgccagct
     2701 cttcatccag caacgccaga ccgcgctcca gagtacgggc aaactgctct tcttcagtct
     2761 tcagcacctg ctcaacctgc gcctgctggc gtttcaggtc ttcacccgca gagcccataa
     2821 cgtcgatcag cggaccaacc agtttgtaga agaaggtttc tttcgcgccg agcatattgc
     2881 cgtgacgcac tgcgcgacga atgatacgac gcagtacata accacggttt tcattggacg
     2941 gcattacgcc atccgcgatc aggaacgcac aagaacgaat gtggtcagcg attacgcgca
     3001 gcgatttatt gctcagatcg gtcgcgccag tgactttcgc taccgcctgg atcaacgtgc
     3061 ggaacaggtc gatgtcatag ttagagttaa cgtgttgcag caccgcagca atacgctcca
     3121 gacccatacc ggtatctaca gacggcttcg gcagcggttc catcgtgcca tctgcctggc
     3181 ggttgaactg catgaagacg atgttccaga tctcaatgta gcggtcgccg tcttcttccg
     3241 gacttcccgg agggccaccc caaatgtggt cgccgtgatc gtagaagatt tcggtgcacg
     3301 ggccgcacgg accagtgtca cccatctgcc agaagttgtc agatgcgtat ggcgcacctt
     3361 tgttatcgcc gatgcgaata atacgttcgc gcgggatccc gacttctttt tcccagattt
     3421 cgtaggcttc gtcgtcgctt tcatagacgg taacccacag acgctctttc ggcagggcaa
     3481 accatttttc gctggtcagc agttcccatg caaactgaat ggcatcgtgt ttgaaatagt
     3541 cgccgaagct gaagttgccc agcatttcga agaaggtatg gtgacgcgcg gtgtaaccga
     3601 cgttttccag gtcgttgtgt ttaccacccg cacgcacgca gcgttgggaa gtggtagcgc
     3661 gggaataatt acgcttgtcg agcccaagga acacatcctt gaactggttc atcccggcgt
     3721 tggtaaacaa caaagttggg tcgttatggg gtaccaggga gctgctggca actacctgat
     3781 gtcccttact atggaaaaag tcgagaaacg cctgacggat ctcagcggtg ctcttgctca
     3841 taattatcct gaaatcaagc taacgaaata tcgccaccag ctccagcgtg tcttaaccgc
     3901 cgggctggta actgaaaagt gggaataaga taagttttct tgactgggaa gtaaaatacc
     3961 gtatgcgttc agtcggcaaa atttcgccaa atatcctgga tatcttccat cagatagcca
     4021 cgatagagca gaaaacgctg gatcttaact ttttctgaaa agacagttgg caaaggttca
     4081 ccatattttc gcgtcgcctg atcgcgcgcc agtgcgcacc agtcgatgtc acattcacgc
     4141 atcgcttttt ctgtcgcttc gcgggaaata cctttctgat tcagttcctg acgaatacgt
     4201 gcaggtccat aacctttgcg gctacggctg gcgataaagc gcgcaacaaa tcggctgtca
     4261 tcgagatagc catgttcatg gcaccaggca ataacgcgct cgtaatcttc tgccgtagca
     4321 tcaatctctt ctgggccatt tttgcccata atcggtgccg cgagtttacg tcgcagttct
     4381 tgctcactgt gatcgcgcac cgccagaatg cgtaccgcac gatccaacag gcgagcatat
     4441 gccgggcgac gggacgttga ttctgtcatg gcatatcctt acaacttaaa aaagcaaaag
     4501 ggccgcagat gcgacccagg ttttgctgat aaacagattt aatagtcaat gagtgtgcag
     4561 aatgttcccg caggcccaat tcgttagagg atcgaggtga gagtaatttt tcacgccagt
     4621 gctggcctgt tcccctcacc ctaaccctct ccccaaaggg gcgaggggac cgtccgtgca
     4681 cacattatct attatcatca ataacgaacg attaaaaatc ttcgttagtt tctgctacgc
     4741 cttcgctgtc atctacagag aaatccggcg ttgagttcgg gttgctcagc agcaactcac
     4801 gtactttctt ctcgatctct ttcgcggttt ccgggttatc tttcagccag gcagttgcat
     4861 tcgctttacc ctgaccgatc ttctcacctt tgtagctgta ccacgcgcct gctttctcga
     4921 tcagcttctc ttttacgccc aggtcaacca gttcgccgta gaagttgata ccttcgccgt
     4981 agaggatctg aaattcagcc tgtttaaacg gtgcagcgat tttgttcttc accactttca
     5041 cgcgggtttc gctacccacc acgttttcgc cctctttcac cgcgccgata cgacggatgt
     5101 cgagacgaac agaggcgtag aatttcagcg cgttaccacc ggtagtggtt tccgggttac
     5161 cgaacatcac accaattttc atacggatct ggttgatgaa gatcagcagc gtgttggact
     5221 gcttcaggtt acccgccagc ttacgcatcg cctggctcat catacgtgcc gcaaggccca
     5281 tgtgagagtc gccgatttcg ccttcgattt ccgctttcgg cgtcagtgcc gccacggagt
     5341 caacgacgat aacgtctact gcgccagaac gcgccagggc gtcacagatt tccagtgcct
     5401 gctcgccggt gtccggctgg gagcacagca ggttatcgat atcgacgccc agtttacgtg
     5461 cgtagattgg gtccagcgcg tgttcagcat cgataaacgc acaggtttta ccttcacgct
     5521 gcgctgcggc gatcacctgc aacgtcagcg tggttttacc ggaagattcc ggtccgtaga
     5581 tttcgacgat acggcccatc ggtagaccac ctgccccaag cgcgatatcc agtgaaagcg
     5641 aaccggtaga gatggtttcc acatccatgg aacggtcttc acccaggcgc atgatggagc
     5701 ctttaccaaa ttgtttctca atctggccca gtgctgccgc caacgctttc tgtttgtttt
     5761 cgtcgatagc catttttact cctgtcatgc cgggtaatac cggatagtca atatgttctg
     5821 ttgaagcaat tatactgtat gctcatacag tatcaagtgt tttgtagaaa ttgttgccac
     5881 aaggtctgca atgcatacgc agtagcctga cgacgcaccg catcacggtc gccgctgaag
     5941 cattcccgcc gggtaatgcc ttcaccgcgg gcagtggcaa aagcaaacca gacggtgccg
     6001 acaggcttct cttcactgcc gccatccggc ccggcgatac cactaataga cacggcataa
     6061 tcagcacgag ccgctttcag tgcgcctatc accatttcca ccacgacggg ttcactcacc
     6121 gcgccatgct gcgccagcgt ctcttcgcgt acgccgatca tctgcgcttt ggcttcgtta
     6181 ctgtaggtga caaatccgcg ttcaaaccag gcggagctac cggcaatatc ggtaatcact
     6241 ttcgctaccc aaccaccggt acaagactcg gcagttgtta cggttgcgcc acgggctttc
     6301 agcgcctgcc caacctgttc acttaactgc atcagttcac tgtcagtcat cacaacctct
     6361 gtcagtcaaa aatttatgcc ggacaagata gcactttcgt caggaagatg gtgttggcgt
     6421 tgcaatttta cgaggaggat tcagaaaaaa gctgattagc cagagggaag ctcacgcccc
     6481 cctcttgcaa atagttactg cacgcgcgcc agcgccacgg cttgtcctaa ctgccagacc
     6541 gccattgcgt aatgggtgct gtggttataa cgggtaatgg tgtagaagtt cggcagaccg
     6601 taccagtact ggtaaccggt accgacatcc agacgcagca ggctggcttg ttgatggttg
     6661 cccagcggct gctgtggcgt taaacctgcc gtggcaagct gcgaaatgct gtacctggtt
     6721 ttgaagccat ttggcaagcc tggagcctga ccgtttgcca ttaccgcgac ctgatcgcct
     6781 ttcacccagc cgtgcgcttt gaaatagttc gccacgctac cgatcgcatc aaccggatcc
     6841 cacaggttga tatgcccgtc gccgctgaaa tctaccgcat attgtttgta agacgacggc
     6901 ataaactgtc cgtagcccat cgccccggca aaggaacctt tcagattgag cggatcgtcc
     6961 tgctcgtcgc gcgccatcaa caggaaggtt tccagctcgc cagagaaata ctccgcgcgg
     7021 cgtgggtagt taaatgacag cgttgccagc gcatcgagga tgcgagtttt ccccatcacg
     7081 cgcccccagc gggtttcaac gccgataatc ccgacgataa tttccggcgg tacgccatac
     7141 acctgccacg cgcgattcaa cgcatcttca tactgattcc agaaaaccac accgttctgc
     7201 acgttgtccg gcgtaataaa ttttttgcga taacggagcc atgcgccgtt cggacctgat
     7261 ggcggtttca ccgatgtggt tggtgcctgg ttatccatca gccgcagtac cgaatccaga
     7321 cgtttcgcct gggagagaat ttcctgcaac tgctgacgat cgaaaccgtg tttgttcacc
     7381 attttgtcga tgaactgctg ggcattcggg ttattagcga aatcgccgcc catctgcatc
     7441 acattgtgct gcggctcaag caggaagccg ccagacggcg ttccggtggt cgtttcagtc
     7501 tcagtaggtt ttggcttgct gctacaggcg gcaagcaaca caaaaagggg aagcaatgtt
     7561 acataacgac gcttgaacat gaggggtcca tttaacagat tcaaccaggg gcaagtatgg
     7621 taaagcatca cgccccacac aaggaagcgg tagtcactgc ccgatacgga cttt
//
LOCUS       DAZFQD010000196         7595 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.196, whole genome shotgun sequence.
ACCESSION   DAZFQD010000196 DAZFQD010000000
VERSION     DAZFQD010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7595
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.196"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..68
                     /locus_tag="ACHKTW_004774"
     CDS             <1..68
                     /locus_tag="ACHKTW_004774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6465653.1"
                     /translation="VDLEKKYQVLLQVKFFTHLLI"
     gene            complement(571..1764)
                     /gene="tyrB"
                     /locus_tag="ACHKTW_004775"
     CDS             complement(571..1764)
                     /gene="tyrB"
                     /locus_tag="ACHKTW_004775"
                     /EC_number="2.6.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HFO6465654.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVAEAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLKQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHIAIFAGAGFEVSTYPWYDEATNGVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     GAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVMCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
     gene            complement(2017..3096)
                     /gene="alr"
                     /locus_tag="ACHKTW_004776"
     CDS             complement(2017..3096)
                     /gene="alr"
                     /locus_tag="ACHKTW_004776"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFO6465655.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            complement(3149..4564)
                     /gene="dnaB"
                     /locus_tag="ACHKTW_004777"
     CDS             complement(3149..4564)
                     /gene="dnaB"
                     /locus_tag="ACHKTW_004777"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFO6465656.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            4647..5630
                     /gene="qorA"
                     /locus_tag="ACHKTW_004778"
     CDS             4647..5630
                     /gene="qorA"
                     /locus_tag="ACHKTW_004778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HFO6465657.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDRVVYAQSALGAYSSVHNII
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGVGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREENLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            complement(5796..6038)
                     /gene="pspG"
                     /locus_tag="ACHKTW_004779"
     CDS             complement(5796..6038)
                     /gene="pspG"
                     /locus_tag="ACHKTW_004779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HFO6465658.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKMPKYQRDDRWRY"
     gene            complement(6172..7209)
                     /gene="dusA"
                     /locus_tag="ACHKTW_004780"
     CDS             complement(6172..7209)
                     /gene="dusA"
                     /locus_tag="ACHKTW_004780"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HFO6465659.1"
                     /translation="MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIERELSQGTYLGHITRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
BASE COUNT         1970 a         1964 c         1835 g         1826 t
ORIGIN      
        1 ttgtagacct ggagaagaag tatcaagttc tattgcaagt aaagtttttc acgcatttat
       61 tgatttagtt aacaaaatca ggggtaagaa agactttatg tatatttgca cgctttgctg
      121 ttttgccgag gaggttaaag gtgattattc tcattacagg acctttttat ttgatattgg
      181 caatcaatat aaggttaaac ttacacaaag cggaaaaaaa gagctctctt taactttaga
      241 atttaacgat actataattg aatctcagaa agtcacaggc aataaggcaa agcatattct
      301 tgaggatata gaaaaattct atcgtaacaa acccgatact tattattaaa tttaataata
      361 gttagatgtg cagaaattat tactccaccc aaaaaaacag gataaggaat tccagataat
      421 taggataagc ttaacaataa aagaactaat aacgtttatt atctctcacg tagaacgatg
      481 gcatcaaaaa aatcatctat aaagaaagag tgaaagcgac gacagtttaa tttcactgca
      541 ggctgggtag ctccagcctg ctttcctgca ttacatcacc gcagcaaacg cctttgccac
      601 acgttgtaca tttgccgtat ttaacccggc gacacacatg cgaccgctgg cgatgagata
      661 gacaccaaat tcttcacgta gtcggtcaac ctgagcggca cttaaaccgg tataactgaa
      721 catgccgcgc tgattaagca gataatcgaa attgcgttct ggcatctctg tgcttaatac
      781 cttcaccagt tcctgacgca ttgccagaat gcgagtacgc atctcttcta cttccgccag
      841 ccagctggct ttcaatgcct cgtcattcag cactgcagcc accacctgcg caccaaaatt
      901 cggcgggctg gagtagttgc ggcgaactgt tgctttcaat tgccccagta cgcggccagc
      961 ggcttcggca tcttcacaca taacagaaag tccgccgacg cgctcgccgt aaagggagaa
     1021 aattttcgag aacgaattgc tcaccagagc gggtaatcca gcgctggcaa tggcgcgaat
     1081 agcgtaggca tcctcttcca taccggcacc aaatccttga taggcaatat cgaggaatgg
     1141 aataagctcg cgggctttga gaatttcaat caccgcatcc cactggtcat tagtgagatc
     1201 ggcacccgtt gggttgtggc aacatggatg cagcaacaca atactgcggg caggtaatgt
     1261 tttcagcgtc gccaacaggt cattaaagcg cacgccgtta gtcgcttcgt cataccaggg
     1321 gtaagtactc acttcgaatc cagccccggc gaatattgct atgtggtttt cccaggtagg
     1381 atcgctgacc cagacgcctg attccgggaa gtagcgtttc aggaaatccg cgcccacttt
     1441 caatgccccg gagccgccaa gggtttgaat ggttgctacg cgctgttgtt tcagtaccgg
     1501 atggtccgca ccaaacagca gcggcgcaat ggcatggcga tagctgttaa gcccttccat
     1561 cggtaaataa agcgaagcgc catgaggctg cgcattcagg cgcgcttccg cctccgccac
     1621 ggctttcagt tgtggaataa ttccgtcttc gttgtagtac agaccgatac ttaaattcac
     1681 tttgtcgctg cgagggtctt ctttaaaacg ctccataagc gtaagaatcg ggtcgccagc
     1741 gtaggcgtca actttttgaa acacgcgatg gttctccagg tttacgggca ggtggttaaa
     1801 acacaataaa ccggaagaag gcgaagatcg agtggatgtt caggagcgaa cggcaattag
     1861 caacagagtg agactcatga caaacgtaca tccgccagag gcacgacctt tataagagca
     1921 tgaaagaaca tcaacttatt gaatttttag gattttattg gccggataag gcattcacgc
     1981 cgcatccggc acagacaatc aaatattaca gaactattaa tccacgtatt tcatcgcgac
     2041 ccttgaagtc aggcgcgtaa taagttcgta agcgcttact ttcgtcattt cagcgatacg
     2101 ttctacgggc aaaccttcgc cccataaaat gaccggatcc ccggctttgt cctgcgcctg
     2161 tggacctaag tctacgcaga tcatatccat cgccacgcgc ccgacaatcg gtacttcgcg
     2221 accgttcacc agcactggcg taccggacgg cgcggcgcgc ggataaccat cgccatagcc
     2281 catcgcgact acgccaagac gggtatcacg ttcgcttacc caggttccac cataaccaac
     2341 aggttctccg gctttatgct cacgcacggc aatcaggctg gaggttagtg acatcactgg
     2401 ctgacagcca aaatcggcac cggtggagcg atcttccagc ggcgagacgc cataaagaat
     2461 gatgcccggg cgcacccagt caaaatgcga ctgtggccac agcagaatgc cacccgatgc
     2521 ggcaatggaa cgttgaccag gtttgccttc gcaaaaggta ttaaagatag cgagttgttt
     2581 ctcggttgcg ccacattttg gttcatccgc gcgcgcaaaa tggctgacga tattcaccgg
     2641 ctgacgaacg tttttgcact gggtcaggcg atgataaaac gcctcagcct gttccggcct
     2701 tacgcccaga cggtgcatac cggtatcgag tttcatccag acggtaaccg gctcgtccag
     2761 gctagcctct tccagcgcag ccagctgttc ttcgttatgc acggcggtat gaaaatgttg
     2821 cgcagaaatc gtcggcagat ctctggcatc aaaaaagcct tcgagtaaca gtacaggttt
     2881 ggtgattccc cccgcacgca gtcgcagagc ttcttcgaga cgggctacgc caaaggcgtc
     2941 agcatcgggg agcgttcgcg cggtctcaag aagaccgtga ccataagcgt tcgctttcac
     3001 caccgcaacc attttactgg caggtgccag ttcacgaaga cgttgcaggt tgtgtcgcag
     3061 agcgcggcgg ttaatcacaa cagttgccgc ttgcatttgt gttccttgat aagtgtttgc
     3121 tttaattacc taattcataa aataattatt attcgtcgtc gtactgcggc cccgcatagt
     3181 tgtcgaagcg cgaccattga ccgttaaagg tcaggcgtac cgtcccgatt gggccgttac
     3241 gttgtttacc gataataatt tccgcgatgc cttttaaatc actgttttcg tgatacacct
     3301 catcacgata gataaacatg atcaagtccg catcctgctc gatagagcca gattcacgca
     3361 ggtcggagtt gaccgggcgt ttgtcggcac gttgttccag agaacggttc aactgggaca
     3421 gcgccaccac cggcacgttc agttctttcg ccagtgcttt cagcgagcga gagatttctg
     3481 caatttccag cgtacggtta tcggaaagcg ccggtacgcg catcagttgc aggtagtcga
     3541 tcatgataag cccgatgccg ccgtgttcac gggcaatacg gcgtgcgcgg gaacgcactt
     3601 ccgttggcgt caggccggag gagtcatcga tatagatatt gcgtttttcg agcaaaatac
     3661 ccatggtgcc ggaaatgcgc gcccagtctt catcatcgag ctgcccggta cggattttag
     3721 tctggtcaac gcgcgacagc gacgccagag aacgcatcat aatctgttct gaaggcatct
     3781 ccagcgagaa gataagtact ggtttatcct gcaacatcgc cgcgttttcg acgaggttca
     3841 tcgcaaatgt tgttttaccc atcgacggac gcgcggcgac gatgatcaaa tccgacggct
     3901 gcaagccagc ggtttttttg ttgagatcgt cataaccggt gtttactccg gtaacgccat
     3961 cgtgtggctg ctgaaacaac tgctcaatac gcgccacggt tgcgtcgagc acatcggcga
     4021 tgttcttcgg cccttcgtct ttgtttgcac gactttcagc aattttaaag acgcgggatt
     4081 cagcaaggtc cagcagatct tcgctggtgc gcccctgcgg atcaaaaccg gcttcagcaa
     4141 tctcattcgc aaccgaaatc atctcacgga caacggcacg ttcacgcacg atgtccgcat
     4201 aggcactgat gttcgccgca cttggcgtat tttttgacag ctctgccaga taagcaaaac
     4261 caccgacgct atcgagttgc ccctggcgtt ccagcgattc cgcaagagta attagatcga
     4321 tagggctacc gctttcctgc aaacgcgcca tttcagtaaa gatatgacgg tgtgggcggg
     4381 tgtaaaaatc gtctgctacc acacgctcgg ctacatcatc ccagcgttcg ttatctagca
     4441 ttaaaccgcc caacaccgac tgctccgctt cgatcgagtg cggaggcact ttcagcccgg
     4501 caacttgtgg atcgcgttcg cggggttcag cctgctgttt gttgaagggt ttatttcctg
     4561 ccatagtgaa tggagttacc gagataaaga atgggtcgaa actttaccat atgaagcaga
     4621 ccctgacgat acgttctgga ggacacatgg caacacgaat tgaatttcac aagcacggtg
     4681 gcccggaagt acttcaagcc gtagagttca ctcctgccga tccggcagag aatgaaatcc
     4741 aggtcgaaaa taaagccatc ggcatcaatt ttatcgacac atatatccgc agcggccttt
     4801 acccgccgcc atcgctaccc agcggattag gcaccgaagc agcaggcatc gtgagtaaag
     4861 tcggcagtgg tgtaaagcat attaaggcag gcgatcgtgt agtctatgcg cagtcggcgt
     4921 taggcgctta cagctctgtg cataacatta ttgcggataa agcggcgatt ctgcctgcgg
     4981 caatttcttt tgagcaagct gcggcatcct tcctgaaagg cttaacggtt tattatctgc
     5041 tgcgcaaaac ctatgaaatt aaacccgatg agcagttcct gttccacgca gcagctggcg
     5101 gcgttggctt gattgcctgc cagtgggcaa aagccctggg cgcgaaactt atcggcaccg
     5161 taggaaccgc gcaaaaagcg cagagcgcgc taaaagcggg cgcgtggcag gttattaact
     5221 atcgtgaaga gaatctggtc gagcggttaa aagagatcac cggcggtaag aaagtgcgcg
     5281 tggtgtacga ttccgtgggc agagacacct gggaacggtc gctggattgc ctgcaacgcc
     5341 gcggcttaat ggtgagtttt ggcaactcat caggtgcggt taccggtgtg aacttaggca
     5401 ttctcaatca gaaaggctcg ttgtatgtga cacgcccttc cctgcaaggc tatatcacca
     5461 cgcgggagga attaaccgag gccagtaatg aactgttctc tttgattgcc agcggtgtga
     5521 ttaaggtcga tgtcgccgag cagcagaaat atccgctgaa ggatgcgcag cgtgcgcatg
     5581 aaattctgga aagccgggca acgcaaggtt ccagcctgct gattccataa aagaaatagg
     5641 gcttccacct gggaagccct ttctttttat agttcggctg tatgtagggt acagcacgat
     5701 gaatctgtta gaggcgcaat agtgacagat ttgattatca attcctattt tgttctaagg
     5761 ataaaacctt aggttgtgat catccgcaca atcccttagt aacgccagcg gtcatcacgc
     5821 tgatatttcg gcatttttgg tgctttaatc gccttaataa cccacaccac cgcaatcgcc
     5881 agtagtaacc acggcagcaa cttaatcatc aatgccagca taccgccgag gaacataatg
     5941 gccgtcgcca caaccagcgc ggcgataatg cccagcaacg aaacgccggt gaccatcagc
     6001 atgacaaaaa agccaatcac aaaaagtagt tccagcatga tgctctccca aatatgaaat
     6061 ctcttgctgg cattacaaga atcatgccaa aaataatcta ttgatttaac agcaaaacgc
     6121 cccgcgacgg tgcgcagggc gtggtgaatt tgactacttt ttggtgaaaa gttaacgctt
     6181 atccgccacc agtttgagcg cgtgttccag cacgttaatg tcagcacccg ctttatgggc
     6241 attttcactt aaataacgcc gccactgccg cgcgccagga ataccctgga acaaacccag
     6301 catatgccgg gtaatatggc cgagatacgt cccctggctg agttcacgct caatgtacgg
     6361 atacatggcg cgcactaccg ccaccggatc ggcatcggta tccgaggaac caaagatctc
     6421 ccggtctacc gccgccagaa tacccggatt ctgatacgcc tcgcgcccga ccatcacgcc
     6481 atccatatgt tgcaggtgtg ctttggcctc ttccagcgac ttgataccac cgttaatcga
     6541 cattgtcaga tgcggaaagt cacgcttcag ttgatacaca cgcggataat cgagcggcgg
     6601 gatttcacgg ttttctttcg ggcttaaccc cgaaagccag gctttacgtg cgtggatgat
     6661 aaacatctca cactcgcctt tgccggaaac agtgttgatg aaatcgcaga gaaattcata
     6721 gctgtcctgg tcatcgatgc caatacgcgt tttcaccgtc actggaatcg acaccacatc
     6781 gcgcatcgct ttcacacagt cggcaaccag ctgcgcatta cccatcagac acgcaccaaa
     6841 catgccgttc tgcacccggt cagacgggca gccgacattc aggttgatct catcatatcc
     6901 acgcgcttct gccagcttcg cacactgtgc cagcgccgcc ggatcgctac cgccgagttg
     6961 caacgctacc ggatgttctt cttcactgta cgccaggtaa tcacctttac cgtgaataat
     7021 cgcccctgtg gtcaccattt cggtatacag caacgtattg cgggaaagca gacgcaagaa
     7081 atagcggcaa tgtctgtccg tccagtcgag cattggtgca acgctaaacc gaccactcca
     7141 gtgaacgtca gttttttcag gcattgcgct ggtttggttg attttttgca tttcagaatt
     7201 accgtgcatt ttcaaatgta gagattattt tatcgatata tcattgggtt atgttattca
     7261 gcatcactgt tcaggaggct caatagcggg gtactatacc ataacaacag gaagcgcctg
     7321 tctcattgca aaagaaaatt gagatcaaat caaggcatga agctctcacg aagtgatgga
     7381 aataatctta ttagccgtta gccttgttaa ggacaatgat aaacaatcca ggttcgacga
     7441 taaataaaaa atcacacatt aaactccggt gatatctctt cctgctaatg gcactgatag
     7501 aagaaaaaag aacccaataa gtattaggtt cttttatgta atgccttcca taccatcgaa
     7561 gaacttcaca tattatttcg ccgatttaac cccga
//
LOCUS       DAZFQD010000197         7394 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.197, whole genome shotgun sequence.
ACCESSION   DAZFQD010000197 DAZFQD010000000
VERSION     DAZFQD010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7394
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.197"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(156..518)
                     /locus_tag="ACHKTW_004781"
     CDS             complement(156..518)
                     /locus_tag="ACHKTW_004781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HFO6465660.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLQEMTGFSKTAF"
     gene            803..1012
                     /gene="yibT"
                     /locus_tag="ACHKTW_004782"
     CDS             803..1012
                     /gene="yibT"
                     /locus_tag="ACHKTW_004782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibT"
                     /protein_id="HFO6465661.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            complement(1024..1611)
                     /gene="mtlR"
                     /locus_tag="ACHKTW_004783"
     CDS             complement(1024..1611)
                     /gene="mtlR"
                     /locus_tag="ACHKTW_004783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HFO6465662.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAFENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISRISLKKAFQK"
     gene            complement(1611..2759)
                     /gene="mtlD"
                     /locus_tag="ACHKTW_004784"
     CDS             complement(1611..2759)
                     /gene="mtlD"
                     /locus_tag="ACHKTW_004784"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008926 - mannitol-1-phosphate
                     5-dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0019594 - mannitol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFO6465663.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYSLPHKNLIQGIAGAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISGLDANSEVVSEAVTAYKAMQ"
     gene            complement(2989..4902)
                     /gene="mtlA"
                     /locus_tag="ACHKTW_004785"
     CDS             complement(2989..4902)
                     /gene="mtlA"
                     /locus_tag="ACHKTW_004785"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HFO6465664.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGERGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGASPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTANEEKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            5439..5801
                     /locus_tag="ACHKTW_004786"
     CDS             5439..5801
                     /locus_tag="ACHKTW_004786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HFO6465665.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATDPEIASLSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            5804..6940
                     /locus_tag="ACHKTW_004787"
     CDS             5804..6940
                     /locus_tag="ACHKTW_004787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFO6465666.1"
                     /translation="MDLLIVLTYVALAWTVFKIFRIPVNQWTLATAALGGVFLVSGLI
                     LLMNYNHPYTFTAQKAVIAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLAPVRYQAR
                     VDRLQADLMTATHNIKTLRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFS
                     ERDIDDARQNFLAQDALVKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLE
                     QTVIRAPSNGYVTQVLIRPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGD
                     DAEVVFNALPGQVFHGKLTSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPND
                     DIDALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWMHYLYLDH"
BASE COUNT         1806 a         1861 c         1837 g         1890 t
ORIGIN      
        1 taaaaatgca ttttcagaat atcaaccagc ggtaaatcga cgtaaaggga gtgaattgtt
       61 tggtaattag gcgagtagac aggagagtcc ccgcattgac cagctaaggc aagccagatg
      121 cgggttgata tgatcgaacc agtcgtctga ggcgcttaaa acgcagtctt agagaatcca
      181 gtcatctcct gcaggcccat ttcacggccc agcgcggtca ttgggtgcac aaccaccagt
      241 ccgcgaacac ttttcttcag cttgcccata tcagcctgct cttttttggt aattgcgcgc
      301 tgaaacggca tcttcatcag cttctgtgct tctttactca gtttttggct atgaacttca
      361 cgcaggcgag caatttctgc ttccagcgtg gctttctctt tttccagctc ggcatatttg
      421 tctgcggctt caaccagcga cagatcggcc tgctggtggc ggatggcgtc cagacgatcg
      481 ctgagacgtt tgatttcgtt tttttcgact tctttcatgt gatggcttct gataaagata
      541 aatttatagc ccctagtatg cgcgaccagg tgctttgttc acaactgatg acattgcaat
      601 agttctgtaa cgtaatcatt aacatgctta aaaactacgc aatttattga ttaattaaga
      661 aatctataga aaaaagataa taaacagcag cggatgatta ttgaggattc agcacgtttt
      721 ccttaatgtc atctttaccg ggaacctaaa ctaaaattgg cggccgcgca ttcccggcgc
      781 ggggtaatac ggagatatca tcatgggcaa attaggtgaa aacgttccgc ttcttatcga
      841 taaagccgta gatttcatgg catcaagcca ggcgttccgg gagtatctga aaaaacttcc
      901 tccccgtaac gcgattccgt ccggaatacc cgatgaaagc gtgccgttat atctacaacg
      961 tctggagtat tatcgtcggc tttatcggcc gaagcaggta gaggggcagt aagcagacgt
     1021 tgcttacttt tgaaaggctt tttttaagct gattctggaa atcagctcag tcagtgaaag
     1081 gaccattgtt gatcgcacag cctgttgata gcgctgaatt tgcattgcat acaaactgga
     1141 gtctgctggt tcaaactgtg gcggcggtgg taacgccgcc acgcagtgca gttcaccaaa
     1201 gggtccaagg atctcgtcgt cggtaaaggc gtactcgttg ccgtcgtgat ttagttcttc
     1261 acgcaatgcc atcagcagct ccgcatcttc gtattcctgg cggttaatga cgcccaaccc
     1321 gtaaatgagt tttaaacgca cagaaagatc gcccagcgga ccgtcgccgt cgagtaacgg
     1381 ttctacagca tacttcaccg cgtaatcgtc tttgcggaat acctgaagca ccagaagatt
     1441 taccgcctcg gtcaaaagct cgacggcggt gatcagaaag cttcgcacgg ttttgccagc
     1501 attcagacgc tcaagcacac ggttttcaaa ggcctgggtt tgttccattg ttgcctgcat
     1561 atctgacaat ctgttattcg ggcgcagggt gtcctgcgcc tggtccacca ttattgcatt
     1621 gctttataag cggttaccgc ctcggataca acctcgctgt tggcatcaag accggaaatc
     1681 tgtgccagcg ccgcctgcgg acctttgtca gcgatcagtg ctgccagttc ctgagcctgc
     1741 ggatcatctt cactgcggaa gtgcattgca ccagcaatcc cctgaatcag attcttgtgc
     1801 ggaaggctgt attccagcgt accgagcaat ggcttgatca gacggtcgcc agcactcagt
     1861 ttacgcagcg gctgacggcc tacgcgctct acatcatctt tcagatacgg gttctcaaaa
     1921 cgaccgagga ttttctggat gtacgccgca tgtttgtctg cgtcaaagcc gtagcgcttg
     1981 atcagtaccg caccactttc ttccatcgca ccttttacca ccgcgcggat tttctcgtcg
     2041 agaatagcgt cacgaatggt ctgatgaccg gccagttttc cgaggtacgc ggttatagca
     2101 tgacccgtgt tcagggtgaa gagtttacgt tcgacaaatg ccatcaggtt gtcggttaac
     2161 tccatgcctg ggatgttcgg cagtgcgcct ttgaactgcg ttttatcgac aatccattcg
     2221 ctgaaagttt ctaccgtcac ttccagcgga tcgttagttg ccgaagccga aggcggtacg
     2281 atgcggtcaa cggcggaatc gacaaagcca acgtgttctt ctacccacgc tttggcgtct
     2341 tccggcaggg cgttcatcac atggcctttc agctgcgtgg taccgcgtac catgttttca
     2401 caggcgatga tgttcaacgg ggattcatta ccttgttctt tgcgtttcac cagccctttg
     2461 gcgatagccg gagcaatacg ttccagcaca accgggccaa cggcggtagt gactaaatcg
     2521 acctgagcaa tcagatcaac gacatcatca ccaatgctgc tgacagcatt gacgccggaa
     2581 acggtatcta cctgctcggt ttcaccaacc acatgaacct gatagctatg acgggcattc
     2641 agggcatcaa gtaccacctg attgacatcg gcaaacgtca gttggatacc cgcgtctgcc
     2701 agcagtttac cgataaagcc acgaccgata ttacctgcgc caaaatgtaa tgctttcata
     2761 gtattaacct tcatcaatgt ttttacccga gagggctggg gtgaggcttt tccctcaccc
     2821 aaaccctctc cgtgaggaga ggggcagcgc cggatgcggc gtaagcgcct tatccggcct
     2881 acaagaacgt gcaaattcaa caaatcgtga tgcatcctgt aggcctgata agcgtagcgc
     2941 atcaggcaat taaccccacc ttctccatgt ggagagggtg ggattggatt acttacgacc
     3001 tgccaacagt tccagcactt catccacgct ggtggtgtgt gccagacgct cgatgacgga
     3061 ctcatcatcc agtgcattgg tcaggctggt gataacctga atgtgctcgt tgttacgggc
     3121 tgcaatacca atcaccagac gggcaatgtc atcttcttct tcaccgaagc gcacgccttc
     3181 cgggtactgg cagaacacca cgcccgtttt cagtacgcga tctttcgctt caaccgtacc
     3241 gtgtggcacc gcgatagact cacccagata agtcggggtc agtttttcac gatccagcat
     3301 cgcctgaacg tattccggct caacgtaacc gcctttcacc agctgctcgc cagcaaaacg
     3361 aatcgcttct tctttggttg ccgctttgcg accgaggaag atgttctccg cgcctagctt
     3421 gaacaggtta gcactggaat cgtcaaagct gtctttcagg ctgtctttta ctttctcttc
     3481 gtttgccgtg tggcgttggg cagcaaccag acgttcggtc aggctggtgt acaggccgct
     3541 gtcgaggaag ttggtcagcg aaatatgctg tgcctgcgga acctggcgca tagcgcgttc
     3601 ggtcaggtca cggtgagtga tgacgaggtc cacatctggc ggcaggttgt tgatcgcgct
     3661 gttagtaaca gaaatctgcg acagacctgc atcctgaatt ttcttacgca gaacgcctgc
     3721 gcccatcgca ctggaaccca taccggcgtc acaggcaacg atgattttac gtacgtggct
     3781 caggtcgtta gtcacatcgc cagcagacag cggagatgcg cctttagact cagctttcat
     3841 gtcctgcata cgacgagttg ctgcttcaat atcatcttct tctttcactt tgctggtttt
     3901 cagcaaaata gcagagacaa cgaaggagac agccatcgcc gcacacacac ccgcgatgtt
     3961 agcgaagtaa gcaccttttg gcgtcatcgc cagtaccgca aggatagacc ccggagatgc
     4021 tggagaaacc agaccaccgc ccaggatagt cagcgtgaac acgccagtca taccgccgag
     4081 gataacggcc aggatcagac gcggattcat cagcacatac gggaagtaga tttcgtggat
     4141 accacccagg aagtggatga ttgccgcacc gcccgcagac tgtttagcac taccacgacc
     4201 aaagaacatg tacgccagca gcacgcccat acctggacct gggttagctt caatcaggaa
     4261 gaagatggat ttacccagtt catgggactg ctgaataccc agcggcgaga agataccgtg
     4321 gttaatggcg ttgttgagga acaggatttt cgccggttca acaaagatag acgccagcgg
     4381 cagcatgtca tggacaacca tgaagttaac gcccgcagcc agcattttgg acagggcttc
     4441 aacaatcggg ccaatgccga gaaatgccag aatggcgagg atcatcccga tgatgcctgc
     4501 ggagaagtta ttcaccagca tctcaaaacc ggatttgatc ttaccgtcta cccagcggtc
     4561 gaagtgctta atgcaccagc cgcccagcgg acctgcaatc atagaaccga ggaacatcgg
     4621 catgtctgcg ccgacgataa cgcccatggt agtgatggca ccgactacgc cgccacgttc
     4681 gccgcctacc agcttaccac cggtataacc gatcagcagc ggcaggagat aagtgatcat
     4741 cggcccgacc agcttcgcca gcgtctcgtt cggtaaccac cctgttggaa taaataacgc
     4801 ggtgatgata ccccacgcga taaacgcgcc gatatttggc atcaccatgt tgctgaggaa
     4861 acgaccaaag ctttgcactt tgatcttaat atcggatgac ataaaaacac cccttcttat
     4921 gtttgctgtc gcgcaggctg gaagcccgag gttttttgtt aatgtggcgg cagaggtagc
     4981 cggaccttgt agatactgcg aaatctggca ctgaatcggt taactgtcca gtcgacggcc
     5041 ttttgtgtga tatcagtcac ataaatatag ggggtagaga ggtaattgac gtgatactca
     5101 tcacaaaacg cttgtgtaaa aaagcaacaa gacggcgatt ttcgctaaaa atggcattta
     5161 aatgtgagta gtgtcacatt tttgttttga tggtttgttg aatctttgtg atctgaatca
     5221 caagatattt tcttacggcg ttcacctgat gtgatctaca gcatgttatg ccttcgtcat
     5281 acaatcgtta tgtaactaag caactcatca cctctaaaaa ttattctttg gtgttatggc
     5341 gagaatcgcg aacccggatt attgtcaaaa tatcagcctg actcccttct ggctgccagc
     5401 ccaaaaacaa cactgataaa cgtgaatccg aggcagatat gtttctaaac tatttcgcgt
     5461 tgggagtgct gatcttcgtc tttctggtga ttttttatgg aatcatcgcg atacatgaca
     5521 tcccttatct gattgccaaa aagcgcaatc acccccatgc cgacgctatt catacggcgg
     5581 gctgggtgag cctgtttact ctgcatgtta tctggccgtt tctgtggatc tgggcgacgc
     5641 tctatcaacc ggagcgtggc tggggtatgc agtcacatgt tgcgtcgcag gagaaagcga
     5701 ctgacccgga aatcgcctca ctttctgacc gaatttcccg gctggagcat caactcgccg
     5761 ccgagaaaaa gactgactat tccacgttcc cggagatcta attatggatc tattgattgt
     5821 tttaacttac gtggcgctgg cgtggacggt ctttaaaatc ttccgcattc cggtaaatca
     5881 gtggacgctg gcgacggcgg cgctgggagg cgtgtttctg gtgagtggtt taattttgtt
     5941 gatgaactac aaccaccctt acacttttac cgcgcaaaag gcagtgatag cgatccctat
     6001 cacgccacag gtgacgggaa ttgttactga agtcactgac aagaataatc agcttattca
     6061 aaagggcgag gtgcttttta agctcgcccc ggttcgttac caggcgcgag ttgacagact
     6121 tcaggctgac ctgatgacgg cgacgcataa tataaagacg ctgcgtgcgc agctcactga
     6181 agcgcaggcc aacaccaccc aggtttcagc ggagcgcgac cgtctgttta aaaattatca
     6241 acgttacttg aaaggcagcc aggcggcggt gaatccgttc tcggaacgtg acatcgacga
     6301 tgcgcggcaa aatttcctcg cgcaggatgc gctggtgaaa ggctcggtgg cggagcaggc
     6361 gcagatccag agccagctcg acagtatggt taacggcgag caatcgcaga ttgtgagctt
     6421 aagagcgcaa cttactgaag caaaatataa ccttgagcag actgtcattc gcgcgccgag
     6481 caatggctac gttactcagg tactgatccg cccaggtaca tacgcagctg ccttgccgct
     6541 gcgtccggtg atggtcttca tccccgagca aaaacggcaa attgtcgccc aatttcggca
     6601 aaactcgctg ttacgtctga aacccggcga tgatgcggaa gtggtgttta acgcgctacc
     6661 tgggcaggtg tttcacggca aactgactag tattttacct gtcgtgccag gcggttctta
     6721 tcaggcgcag ggggtattgc aatcattaac ggtcgtgccc ggcacggacg gtgtgctggg
     6781 aaccattgaa ctggacccta acgatgatat cgatgcctta cccgacggca tctacgccca
     6841 ggtggcggtt tactccgacc atttcagcca tgtttcggtg atgcggaaag tgctgctaag
     6901 aatgaccagc tggatgcatt atctttattt ggatcattga gggggagggg ggagattatg
     6961 gaactttgct ccttgtaagg actaattatt tttgcatatt acttcattag ctgcgatgat
     7021 ttttctttaa tattcattat gttaataagt tctggatgag tgtgctctca ttggattttt
     7081 tatccgtctt gttctgccgc aggatgagac tatacatcgt cgagatcgca atgatgatat
     7141 gagttgttcc tcctgcgacg gctctaatca aaaaagagca ggagtgcttt tccatctatt
     7201 gcgatgatgg ctttattatc tgaaggatga cagtcacatt taaaaaatat ttaatctata
     7261 gatcacaatg tagggtcatg gaatatatgt atacaactat tttcttcttc acttttaatt
     7321 atcgtattca ttataaaact aagttaaaaa aactcctgga gagtgagtta ttaatatatt
     7381 tttaaataaa atta
//
LOCUS       DAZFQD010000198         7325 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.198, whole genome shotgun sequence.
ACCESSION   DAZFQD010000198 DAZFQD010000000
VERSION     DAZFQD010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7325
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.198"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            24..554
                     /locus_tag="ACHKTW_004788"
     CDS             24..554
                     /locus_tag="ACHKTW_004788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6465667.1"
                     /translation="MSQGKEPVIERIGMLTKRYPSRSAAARAWGININTLNSYFKGGQ
                     TQPAPRESILIRISESEGVSLEWLKEGKGELPNDIGIYGNFGCSGDKGDQLTQMLSFL
                     TKDEREQLTNLLARKGVETVLTLLNKENQDLLQLEGRKRLAALMIVDMPDDCVREILE
                     KTETSRQSNPVESKAG"
     gene            920..2356
                     /gene="nrfA"
                     /locus_tag="ACHKTW_004789"
     CDS             920..2356
                     /gene="nrfA"
                     /locus_tag="ACHKTW_004789"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HFO6465668.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHPDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEELARKNGLLSQ"
     gene            2401..2967
                     /gene="nrfB"
                     /locus_tag="ACHKTW_004790"
     CDS             2401..2967
                     /gene="nrfB"
                     /locus_tag="ACHKTW_004790"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HFO6465669.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAAQASDDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQPSPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            2964..3635
                     /gene="nrfC"
                     /locus_tag="ACHKTW_004791"
     CDS             2964..3635
                     /gene="nrfC"
                     /locus_tag="ACHKTW_004791"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HFO6465670.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            3632..4588
                     /gene="nrfD"
                     /locus_tag="ACHKTW_004792"
     CDS             3632..4588
                     /gene="nrfD"
                     /locus_tag="ACHKTW_004792"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HFO6465671.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPILPVLFLFSGISSGAAVALIAMAIRQRSNPHSTEAQFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            4608..6326
                     /gene="nrfE"
                     /locus_tag="ACHKTW_004793"
     CDS             4608..6326
                     /gene="nrfE"
                     /locus_tag="ACHKTW_004793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HFO6465672.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQTDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDGACARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQREIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPVVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRVQLPALVAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFHFERLDLQAKGNYTSEKAIVALFDHQQRIGELMPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTGADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GKKRDE"
     gene            6319..6702
                     /gene="nrfF"
                     /locus_tag="ACHKTW_004794"
     CDS             6319..6702
                     /gene="nrfF"
                     /locus_tag="ACHKTW_004794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HFO6465673.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVAEGKNEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            6699..7295
                     /gene="nrfG"
                     /locus_tag="ACHKTW_004795"
     CDS             6699..7295
                     /gene="nrfG"
                     /locus_tag="ACHKTW_004795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HFO6465674.1"
                     /translation="MKQPKIPVKMLTTLTILMVFLCVGSYLLSPKWQAVRAEYQRQRD
                     PLHQFASKQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
BASE COUNT         1646 a         1828 c         2075 g         1776 t
ORIGIN      
        1 gaaaagcacc aagaggtatc tttatgtcgc agggaaaaga acctgttatt gaacgtattg
       61 gaatgcttac aaagcgctac ccttcaagaa gtgcagctgc cagagcgtgg ggaatcaata
      121 tcaacacatt aaacagctat ttcaagggag gacaaacaca accagccccc agagaaagta
      181 tccttatacg aatttctgaa agtgaaggcg ttagtcttga gtggcttaaa gagggcaagg
      241 gtgaattacc aaacgatatt ggtatttatg gtaattttgg ttgttctggt gataagggag
      301 atcagcttac acagatgctt tcattcctta cgaaagacga acgagaacag ttaaccaatc
      361 tccttgctcg caaaggtgta gagacggttc tgacactgct gaataaagaa aaccaagatt
      421 tattacaact agaaggcagg aagcgcctgg ctgcgttgat gattgtagat atgccagacg
      481 attgcgtcag agagattttg gaaaaaaccg agacaagtag gcaatcaaat ccggtagaat
      541 ctaaagcggg ttagtttggc gaggctggcc cgtatgttag ttgccatata gatttaatga
      601 agttttaaac gctttaaaaa gttgagatcg cagtatcaaa agttttgccg attttagaat
      661 aaacgattaa ttattgcaca atcgatatca aatagacttt aagcgctcat ccagacatca
      721 cctgttttat ttcagtatct tacaggtgat ttcacctcct ttcactaaaa ccctcgttgg
      781 tatcaaatga ttcacctggt tacaggttat ttgttacaca taggggcgag caatgtcatg
      841 acagtgtagg tgcggttact gtcgtgaaaa acaataaaaa ccgccattgc aacaatggcg
      901 caattcggat gaagccccta tgacaaggat aaaaataaac gcacgccgta tcttcagctt
      961 attgattcct tttttctttt tcacttctgt tcacgctgaa caaacggctg ctcccgcaaa
     1021 acctgtaact gtggaagcga agaatgaaac ctttgccccg cagcatcccg atcaatatct
     1081 ctcctggaaa gccacctcgg aacagtcaga gcgtgttgac gccctggcgg aagatccacg
     1141 gctggtgatc ctgtgggcgg ggtatccctt ctcgcgcgat tacaacaagc cgcgtggaca
     1201 tgcctttgct gtgaccgatg tgcgtgaaac cctgcgtacc ggtgcgccga aaaacgctga
     1261 agatggtccg ctaccgatgg catgctggag ttgtaaaagc ccggatgtgg cgcgtctgat
     1321 ccagaaagac ggcgaagatg gctacttcca cggtaaatgg gcgcgcggcg gcccggaaat
     1381 cgtcaacaac ttaggttgtg ccgactgcca taacaccgcc tcaccagagt tcgccaaagg
     1441 caaaccggag ttaacccttt cccgtccgta tgcggctcgc gcgatggaag ccattggtaa
     1501 accttttgag aaagccggac gtttcgacca gcaatcgatg gtttgcggtc agtgccatgt
     1561 ggagtattac ttcgacggca aaaacaaagc ggttaaattc ccgtgggatg acggcatgaa
     1621 agtcgaaaat atggagcagt attacgacaa aattgccttc tctgactgga ctaactccct
     1681 gtcgaaaacg ccaatgctga aagcgcagca cccggaatat gaaacctgga cagcgggcat
     1741 tcacggtaaa aacaacgtga cctgtatcga ctgccatatg ccaaaagtgc agaacgccga
     1801 aggcaaactc tacaccgacc ataaaattgg taatccgttt gataacttcg cccagacttg
     1861 tgcgaactgc catacccagg acaaagctgc cttgcaaaaa gtggtcgcgg aacgtaagca
     1921 gtcgattaac gacctgaaaa tcaaggttga agatcaactg gttcacgctc acttcgaagc
     1981 gaaagcagcg ctggatgcag gcgcgacgga agccgaaatg aagccaattc aggacgatat
     2041 ccgtcatgcc cagtggcgct gggatctggc gatcgcttcc cacggcattc atatgcacgc
     2101 accggaagaa ggtttacgga tgctcggtac ggcgatggat aaagcggcgg atgcacgcac
     2161 caaactggcg cgcctgctgg cgaccaaagg catcacccat gaaatccaga tcccggatat
     2221 ctcaaccaaa gagaaagccc agcaggctat tggcctgaac atggaacaaa tcaaggccga
     2281 gaagcaggac ttcatcaaaa cggtgatccc gcagtgggaa gaactggcac gtaaaaacgg
     2341 tctgttaagc caataacccc gttccgcctc gcaaggggcg gaaaacacaa tggagtgaat
     2401 atgagcgtat tacgttcgtt attaactgcc ggggtgctgg cgtcgggcct gttgtggagc
     2461 ctgaacggaa ttaccgctac ccctgcggcg caggcatctg atgatcgcta cgaagttacc
     2521 cagcagcgta acccggatgc cgcctgtctg gactgtcata agccagatac cgaaggtatg
     2581 catggaaaac atgcttccgt tatcaacccg aataacaaac tgccggtcac ctgcaccaac
     2641 tgtcacggcc agccatcacc gcaacaccgc gaaggggtga aagatgtgat gcgctttaac
     2701 gagccgatgt acaaggttgg agagcagaac agcgtctgta tgtcctgtca tctgccggaa
     2761 cagttgcaaa aagcgttctg gccgcacgat gtccacgtaa ccaaagtggc gtgcgccagc
     2821 tgccattccc tgcatccaca gcaagatacg atgcagacgt taagtgacaa aggacggatt
     2881 aagatttgcg tcgattgcca cagcgatcag cgcaccaatc cgaactttaa cccggcgtct
     2941 gttccgttgc ttaaggagca gccatgacct ggtctcgtcg ccagtttctc accggcgtcg
     3001 gcgtgctggc agccgtcagc ggcaccgcag ggcgcgtggt ggcgaaaacg ttgaatatca
     3061 atggggtgcg ttacggcatg gtgcatgacg agtcgttatg catcggctgt accgcctgta
     3121 tggatgcttg tcgggaagtg aacaaggtgc cggaaggcgt ctcgcgcctg acgattattc
     3181 gtagcgagcc gcagggcgaa tttcctgatg tgaaatatcg cttcttccgt aagtcttgcc
     3241 agcactgcga tcatgcgccg tgcgttgacg tctgcccgac cggtgcgtct tttcgtgatg
     3301 ccgctagcgg gattgtcgat gttaatcctg atctctgcgt cggttgtcag tactgcatcg
     3361 ccgcctgtcc gtaccgcgtg cgctttatcc atccggtcac gaagacggcg gacaaatgcg
     3421 atttctgccg taaaaccaat ttgcaggccg gtaagctgcc cgcgtgcgtt gaggcttgcc
     3481 cgacgaaggc gctgacgttt ggcaatctgg acgatcctaa cagtgagatt tcgcaactgc
     3541 tgcgccagaa gcctacttac cgctacaagc tggcgctggg aaccaaaccg aagctgtacc
     3601 gcgtaccgtt taaatacggg gaggtgagcc aatgacgcag acttccgcat ttcattttga
     3661 atcgctggtg tgggactggc cgattgccat ctacctgttt ttgattggta tttctgccgg
     3721 tctggtgacg ctggccgtgc tgttacgtcg cttctacccg caggcgggcg gtgcagacag
     3781 tacgttgctg cgcaccacgc tgattgtcgg gccgggcgcg gtgatcctcg gtctgttgat
     3841 cctcgttttc cacctgacaa gaccgtggac cttctggaag ctgatgttcc actacagttt
     3901 tacctcggtg atgtcgatgg gggtgatgct gtttcagctc tacatggtgg tgctggtgct
     3961 gtggctggcg aaaatctttg aacatgattt gcttgccctg caacaacgct ggttgccgaa
     4021 gctggggatc gtgcaaaagg ttctgagcct gctgacgccc gttcatcgcg gactggaaac
     4081 attgatgctg gtgttggcgg tgttgttggg ggcttatacc ggctttctgc tgtcggcgct
     4141 gaaatcgtat ccgttcctca ataacccgat cctgccggtg ctgttcctct tctccggcat
     4201 ctcgtccggt gcggcggtgg cgctgatcgc catggcgata cgccaacgca gtaacccgca
     4261 ttccacggaa gcgcagtttg tacaccgtat ggaaatcccc gtggtatggg gtgaaatctt
     4321 cctgctggtg gcgttttttg tcggtctggc gctgggcgat gacggtaaag tgcgtgcgct
     4381 ggtggcggca ttaggtggcg gtttctggac gtggtggttc tggcttggtg tcgccgggct
     4441 ggggctgatt gtgccaatgt tgctcaaacc gtgggtcaat cgcagttccg gcattcctgc
     4501 cgtgctggcg gcgtgtgggg ccagtctggt cggcgtgttg atgctgcgct ttttcattct
     4561 ctacgccgga cagttgacgg tggcgtaagc cagaaaagag gtggtttctg gacgtattcc
     4621 ttcctgaagt cggttttctg gcgttgttgt taagtctcgg ggtcaacgtg ttgaccccgt
     4681 tgacggcctt tgcgggagtg cggttgcgct ggcctgccat gatgcgactc acttgcatcg
     4741 gcattctggc gcagttcgcg ctcctgctgc tcgcctttgg cgtactgacg tattgttttc
     4801 tcatcagcga tttctcggtc atttatgtcg cgcaacatag ctatagcctg ctgtcgtggg
     4861 aactcaagct ggcagcggtg tggggcggcc atgaaggttc gctgctgctt tgggtgctgc
     4921 tgctttccgc ctggagcgcg ctgtttgcct ggcattatcg gcagcaaacc gatccgctat
     4981 ttccgctgac gttagccgtt ttatctctca tgctcgccgc actgctactg tttgtggtgc
     5041 tgtggtccga tcccttcgtg cggatatttc caccagcaat cgaaggccgc gatctcaatc
     5101 cgatgctgca acatcccggt cttatctttc atccaccgct gctttacctt ggctatggcg
     5161 gtttgatggt agcggcgagc gtggcgctgg cgagtttact gcgcggcgag tttgatggtg
     5221 cctgcgccag aatttgttgg cgctgggcac tacctggctg gagcgcatta acggcgggga
     5281 tcatcctcgg ttcctggtgg gcctattgcg aactgggctg gggcggctgg tggttctggg
     5341 atccggtgga aaacgcctct ttattaccct ggctttctgc cactgcgctg ctgcacagtt
     5401 tgtccctgac acgccagcgg gagattttcc gccactggtc gctgttgctg gcgatagtaa
     5461 ctctgatgct gtcgctactg ggtaccttaa ttgtccgttc tggcattctg gtttcggttc
     5521 atgcgttcgc actggataac gtccgcgccg tgccgttgtt cagcctgttt gcactgatta
     5581 gccttgcgtc tctggctctg tatggctggc gagcgcggga cggtggcccg gtggtgcgtt
     5641 tttcggggtt atcgcgggaa atgttaatcc tcgctacgct gttgctgttt tgcgcagtgc
     5701 tactgatcgt gctggtggga acgctttatc cgatgattta cggtctgctg ggctggggac
     5761 gcctctccgt tggcgcgccg tattttaacc gcgcgacgtt accgtttggc ctgttgatgc
     5821 tggtggtgat tgtgctggcg acgtttgtct ctggcaaacg cgtgcagctt ccggcgctgg
     5881 tagctcatgc aggcgtgctg ttatttgccg cagggatcgt ggtttccagc gtcagccgtc
     5941 aggagatcag cctgaattta cagccgggtc agcaggtgac gctggcagga tacaccttcc
     6001 attttgagcg cctcgatctg caagccaaag gcaattacac cagcgaaaaa gcgatagtgg
     6061 cactgtttga ccatcagcaa cgcattggtg aactgatgcc ggagcggcgt ttttacgaag
     6121 cacgtcgtca gcaaatgatg gaaccgtcaa ttcgctggaa cggcatccat gactggtatg
     6181 cggtcatggg tgaaaaaacc ggagcggatc gttacgcttt tcgtttgtat gtacaaagcg
     6241 gtgtgcgctg gatctggggg ggaggattgt tgatgattgc gggcgcattg ttaagcggat
     6301 ggcgggggaa gaagcgcgat gaataaaggg cttctcacgc tactgctgtt atttacctgt
     6361 tttgcgcgcg ctcaggtcgt agacacctgg caattcgcca atccgcaaca acagcaacag
     6421 gcgttaaata ttgccagcca gttacgttgt ccgcagtgcc agaatcaaaa cttactggaa
     6481 tccaacgcgc cggtggcagt cagtatgcgc catcaggttt acagcatggt ggcggagggg
     6541 aagaacgaag tcgaaatcat tggctggatg accgaacgct acggagattt tgttcgctat
     6601 aacccaccgt taacgggtca gacgctggtg ttgtgggcgc tgccagtggt gttgttactg
     6661 ctgatggcac tgatcctctg gcgagtgagg gcgaagcgat gaaacagccc aaaataccgg
     6721 tgaaaatgct gacaacactc acgatattga tggtatttct ctgtgtcggc agttatctgt
     6781 taagcccaaa atggcaggcg gtacgtgcgg agtatcagcg tcagcgcgat ccgctacatc
     6841 agtttgccag caagcaaacc ccggaagcgc agcttcaggc attacaggat aaaatccgcg
     6901 ctaatccaca aaacagcgaa caatgggcgt tactgggcga gtactatctg tggcaaaacg
     6961 attacagcaa ttcgctgctg gcgtaccgtc aggcgttgca actacgtggt gagaacgctg
     7021 aactgtatgc ggcgctggcg acggtgcttt attaccaggc cagccagcat atgaccgccc
     7081 agactcgcgc aatgatcgac aaagccctcg cgctggactc taacgaaatc accgctctga
     7141 tgctgctggc ttccgatgcg tttatgcagg cgaattacgc gcaagccatc gaactatggc
     7201 aaaaagtgat ggatctcaac tcaccccgga tcaaccggac acagctggtc gagtcgatta
     7261 atatggcgaa attgttgcag cggagatcag attaatcatc tggtgatgaa gattatgtta
     7321 ttgag
//
LOCUS       DAZFQD010000199         7017 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.199, whole genome shotgun sequence.
ACCESSION   DAZFQD010000199 DAZFQD010000000
VERSION     DAZFQD010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7017
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.199"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..167)
                     /locus_tag="ACHKTW_004796"
     CDS             complement(<1..167)
                     /locus_tag="ACHKTW_004796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6465675.1"
                     /translation="MALTHHELCQIAYKFLKRNGFKVCFHDRFVAVTSTGEQPDAMGF
                     RNSASCLIEAK"
     gene            complement(169..606)
                     /locus_tag="ACHKTW_004797"
     CDS             complement(169..606)
                     /locus_tag="ACHKTW_004797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFO6465676.1"
                     /translation="MSKIDYQALREAAQNYQSTLAWYQEKPDSPNAEQDCDAALAAFK
                     RHIRHREADIIADLLDGLEEAKSQLNEQREYYEGVISDGSKRIAELEAREVQLPTRYD
                     LRYGHPINADERQVMIPKENGSWLYLIDLEHALRVAGIRIKGE"
     gene            complement(593..910)
                     /locus_tag="ACHKTW_004798"
     CDS             complement(593..910)
                     /locus_tag="ACHKTW_004798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6465677.1"
                     /translation="MTITKQRVEEIISRIGMYGHGAGYTADEVNDLAVLALNLSNIAN
                     LKRYELDMGGCDSCGQDCGTDMTEDPDGDYVLFDDVVKLFEFDTTTQKLEIPAKEAAS
                     EQD"
     gene            complement(822..1181)
                     /locus_tag="ACHKTW_004799"
     CDS             complement(822..1181)
                     /locus_tag="ACHKTW_004799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4752 family protein"
                     /protein_id="HFO6465678.1"
                     /translation="MNIDPAITIDTALNTGLALLGYFYIMFCSGRWMSLLFMKKWNKR
                     RRQDQRQQAMDAFFEAFGIDGMEPGDPARAISRGGVVILVYRSEEKNDDHKTTCRRNH
                     IPHWNVWTWCRVYRRRG"
     gene            complement(1171..1467)
                     /locus_tag="ACHKTW_004800"
     CDS             complement(1171..1467)
                     /locus_tag="ACHKTW_004800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001267107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4406 domain-containing protein"
                     /protein_id="HFO6465679.1"
                     /translation="MRIYIAGPMTGYENFNREAFHKAAEALKREGHTVLNPAVLPDGL
                     TQPHYMDICMAMLRCVDAVYILKGWQQSAGARTELALTEKLGHAVIFQEVVSEY"
     gene            complement(1464..1781)
                     /locus_tag="ACHKTW_004801"
     CDS             complement(1464..1781)
                     /locus_tag="ACHKTW_004801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO6465680.1"
                     /translation="MSGWKKWRWAEILILRQCAGTMRVESIGYLIGRSESAVRTKARE
                     LGISMMLRGDYHQSAKFSQRDIELARQLHQRGVPQREIAEKFGMKLGAVNNYVYFDRR
                     VQE"
     gene            complement(1778..2494)
                     /locus_tag="ACHKTW_004802"
                     /pseudo
     CDS             complement(1778..2494)
                     /locus_tag="ACHKTW_004802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309538.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
     gene            complement(2516..3262)
                     /locus_tag="ACHKTW_004803"
                     /pseudo
     CDS             complement(2516..3262)
                     /locus_tag="ACHKTW_004803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309101.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            complement(3269..4237)
                     /locus_tag="ACHKTW_004804"
     CDS             complement(3269..4237)
                     /locus_tag="ACHKTW_004804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO6465681.1"
                     /translation="MSSKLHGLVWEGCAFTGMILSRVAVMARLADYSNDEGVSWPAIE
                     TIRRQIGARSESTVKSAIAELAKEGWLTKEERKVGGRNVSNIYRLNVEKLEAAATAAR
                     EAYKPKRKISPAKNDPLTVNPSNIAPSTVDPSNVDGSTVDKKLRIRGAMIDPDPSVLK
                     PDPSDKRSSCPDVSLLDEKRSSPVERFLEKHPDAHTWNVPKRQWGTREDVACAQWIWG
                     RVVALYEQAASDDGEVSRPREPNWTTWANDVRMMRMLDGRSHRQICEMFGRVQRDSFW
                     VKNIMSPAKLREKWDELVIRLGRSPAQRCVNHISEPDTEIPPGFRG"
     gene            complement(4260..4685)
                     /locus_tag="ACHKTW_004805"
     CDS             complement(4260..4685)
                     /locus_tag="ACHKTW_004805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFO6465682.1"
                     /translation="MKIKHEHIRMAINAWAYPDGEKVPAAEIARTYFELGMTFPELYD
                     DSHPEALARNTQKIFRWLDKDTPDAVEKMQALLPAIEKAMPPLLVARMRSHSSEYYRE
                     IVERRDRLVKDVDDFVASAVVLYDQMNRGGPAGNAVVMH"
     gene            complement(4669..4950)
                     /locus_tag="ACHKTW_004806"
     CDS             complement(4669..4950)
                     /locus_tag="ACHKTW_004806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000391949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdaS family helix-turn-helix protein"
                     /protein_id="HFO6465683.1"
                     /translation="MEELRIFLNSLSSDEQRMFACECGTSIGYLRKALSKGQVLGASL
                     CVLIERASNGEVTRQQLRPFDWMNIWPELEDTKTLTQPLSRSLIHENQA"
     gene            5051..5470
                     /locus_tag="ACHKTW_004807"
     CDS             5051..5470
                     /locus_tag="ACHKTW_004807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000362153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6465684.1"
                     /translation="MDKYEFRRQQLIKIRDEKCDGKAVNVARKIGREPSYVSRMLYPE
                     GKKGKKRIADDMVEIIEESFGLPRGWMDGIVSSSTNTASNYETRVLTPRQRIFLDLLD
                     ELPESEADNLLKTLEEKKQYYNMIYEEIRKKKAQNAS"
     gene            5737..5889
                     /locus_tag="ACHKTW_004808"
     CDS             5737..5889
                     /locus_tag="ACHKTW_004808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFO6465685.1"
                     /translation="MDTIELGNNESLVCGVFPNQDGTFTAMTYTKSKTFKTEAGAHRW
                     LARNAN"
     gene            6362..6550
                     /gene="dicB"
                     /locus_tag="ACHKTW_004809"
     CDS             6362..6550
                     /gene="dicB"
                     /locus_tag="ACHKTW_004809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HFO6465686.1"
                     /translation="METLLPNVNTSEGCFDIGVLLSNKAFTEDAINMRKYEPYLLNDN
                     SILSRIALIKLGIFGERQ"
     gene            6607..6735
                     /locus_tag="ACHKTW_004810"
     CDS             6607..6735
                     /locus_tag="ACHKTW_004810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001492811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HFO6465687.1"
                     /translation="MITGIYASKESCLQARDEQKISGECLPVKKVSLNLNNETPAG"
     gene            6831..>7017
                     /locus_tag="ACHKTW_004811"
     CDS             6831..>7017
                     /locus_tag="ACHKTW_004811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HFO6465688.1"
                     /translation="MNKIFICAAIPDEQAIEEDSAVAVATAIEAGDERRARAKFHWQF
                     LEQFPAAQDCAYKFIVCE"
BASE COUNT         1737 a         1908 c         1528 g         1844 t
ORIGIN      
        1 cacttcgcct ctatcaggca tgatgctgaa tttctgaatc ccatagcatc tggctgttct
       61 ccggtactgg ttacagcaac aaagcggtca tgaaagcaaa ccttgaaccc gttgcgctta
      121 aggaacttgt acgcaatctg acagagttcg tggtgtgtta acgccatatc actctccttt
      181 gatgcgaatg ccagcgacgc gtaatgcgtg ttctaggtca atcaggtaaa gccaactgcc
      241 attttcttta ggtatcatga cttgtcgctc atctgcattt atcgggtgtc catatcgaag
      301 gtcgtaacga gtcggtaatt gaacttcccg cgcttccagt tcagcaatac gcttgctccc
      361 atcagagata acgccttcgt aatactcacg ctgctcgttg agttgtgatt ttgcttcttc
      421 cagtccatcc agcaaatcag cgataatatc cgcttcccga tgacggatgt gacgcttaaa
      481 cgccgctaaa gctgcatcac aatcctgttc agcgtttgga ctgtctggct tttcctgata
      541 ccacgccagc gtcgattgat agttttgtgc tgcctcacgc agtgcctgat agtcaatctt
      601 gctcactggc tgcctccttt gccgggattt ctaacttttg agtggttgta tcaaattcaa
      661 acaacttaac cacgtcatca aacaggacat aatcaccatc aggatcttca gtcatatcag
      721 tgccacaatc ctgaccgcac gagtcgcaac cacccatatc aagctcgtat cgcttcaggt
      781 tcgcgatatt tgataaattc agcgccagta cagccagatc attaacctcg tctgcggtat
      841 accctgcacc atgtccatac attccaatgc gggatatgat ttcttctaca cgttgttttg
      901 tgatcgtcat ttttctcttc actccgatat acaaggatta ctacgccccc tctgctgatt
      961 gcgcgagctg gatctcctgg ttccataccg tcaatcccga aggcttcgaa aaatgcatcc
     1021 attgcctgct ggcgttgatc ctgcctacgg cgtttattcc attttttcat gaacaacagt
     1081 gacatccatc gtccgctgca gaacatgatg tagaaataac caagaagtgc caggccggta
     1141 ttcagggccg tatcaatcgt tatcgccggg tcaatattca ctgaccacct cctgaaaaat
     1201 aaccgcatga cccagtttct ccgtcagtgc cagctcagtc cttgcacctg ccgactgctg
     1261 ccagcctttc agtatgtaaa ccgcatccac gcaacggagc attgccatgc aaatatccat
     1321 gtagtgtggc tgagtcagcc cgtccggaag cacagccgga tttaaaacgg tatgcccttc
     1381 ccgtttcagc gcctctgccg ccttgtgaaa tgcctcgcgg ttaaaatttt catatcccgt
     1441 catcggaccg gcgatataaa tcctcactcc tgaaccctcc tgtcgaaata aacgtagtta
     1501 ttcactgcgc ccaacttcat cccaaacttt tcggcaattt cccgttgggg tacgccacgc
     1561 tgatgcagtt gccgcgccag ctcaatatca cgctgtgaga atttggctga ctggtgataa
     1621 tcaccccgta acatcatgct gatccccagt tcccgcgctt tcgtcctgac ggctgactca
     1681 ctacggccaa tcagataacc gatgctttcg actctcatcg ttcccgcaca ctgccggagt
     1741 atcaggattt cagcccaacg ccacttcttc cagccactca ccgctgctgc tctctggtgg
     1801 tggtaatatc ccggagaata tccctgtgtt tgttcagttc ccgcagcgca gcacagactc
     1861 gctcccactt ctggacatca cttttcgccc gacgcagttc gcggtttgcc atatgcagct
     1921 atggtaaaac caggtcatcc gcttgcgttt cggtgaacag tggcaacgac tgcacaatat
     1981 ccaccacagt ttctgtttta atatcttcct gtgtagtcgt ttgctggact ggtaacgcaa
     2041 cacctgctgg ctgaggaagg gctttaccat ccgtttccgc tacagatgcg gctttcggct
     2101 ctactggtaa attatcgccc ggcatgcagt aacgaaattt accgccctga ttcacgcgaa
     2161 tcagacgccc tttgctgatt gccattgcca gcgatgaatt cgcacggcgg gaggtaattc
     2221 cgaacatcaa tgccagctca tccgccgttt gtgggccatg ttgttcaatc gcctctgtca
     2281 gcatttgcgc cgtcactttt ggtggttgta tcacagattc actttcccct gcctgagtca
     2341 gccaccacat cgcccccctg ttatccgctt caccacggcg tttcagtttc cacagttcgt
     2401 tgacagcctc ttcacgactg attccaaggc gcgcagccac cacatgtgaa gaggcttttt
     2461 tcagtgcttt cagtgcgtca gatacggttt ccattaaaat ttcctccgga caaaattact
     2521 tcacaaccct catattgctg acatttggac gccagctatc ccagttaaac gtcacccatc
     2581 gaccaccgtt catggtcatg cggtccataa tcctctcacc aagaagcgta ctcattgcgg
     2641 catgattcag gtttgttaac atcccgacac tgcacagtga tgctgtccgg cgatcaatta
     2701 tctggtgcaa taccacctgc tcgtttttcg tctcccgctg gacgcctatt tcatccagga
     2761 ccagcaaatc aaccccgcaa agctcctgta aaaatttttc cccggatttg ccgttgtcgt
     2821 agctgtcatg caacacgctc atgacgtcag acacggtgac gataatcacg ctgcgcccct
     2881 tcaccatcag ccggttgccc atcgccgctg caaggtgatt tttcccggtg ccggttttac
     2941 cgctgaacac aaaattcgtg cacccggtca tcagttcgtc agctatggat ttggcctagc
     3001 tcagcgcgta tttttgcccg tcgttctgca cctgataatt tgcaaacgag catttgctgt
     3061 gcagaggctg gatgcccgaa cgattcagga ttttttccac ccgcaactgg cgattctggc
     3121 agttaatctc ctcgctgcgt tttcgtcctt cagcaagttg ccattcccgc cactcctcca
     3181 ccgtccggta cggtggaacc gcccccagtg gtgcaagtct gcgaatacat tcaagaaccc
     3241 caactgccgc aatgtttttc atgccacatc accccctgaa tcccggcggt atttcagtgt
     3301 ccggttcaga aatgtgattt acgcaacgct gcgcgggcga acgccccagg cggataacca
     3361 gttcatccca tttttccctg agttttgccg gactcatgat gttttttacc cagaacgaat
     3421 cccgctggac acgcccaaac atttcacaaa tctgccggtg actacgtcca tccagcatac
     3481 gcatcatgcg cacatcattc gcccaggtcg tccagttagg ttctctgggg cgtgacacct
     3541 caccatcatc actggcggcc tgttcataca acgccacaac ccgtccccaa atccactgcg
     3601 cacaggcgac atcctcacgg gtaccccact gtcgcttcgg tacattccag gtatgcgcat
     3661 ctgggtgttt ctccagaaat cgctcaactg gtgatgatcg tttttcgtcc agcagtgaaa
     3721 cgtccggaca agaagatctt ttatctgacg gatcaggttt taatactgac ggatcggggt
     3781 caatcatcgc ccccctaata cgcagttttt tatcaaccgt tgatccatca acatttgacg
     3841 ggtcaaccgt tgagggggca atatttgacg ggttaactgt taacgggtca ttttttgccg
     3901 ggctaatttt tcttttcggt ttatatgcct cacgcgccgc agttgcagct gcttcgagtt
     3961 tttccacatt aagccgatag atattgctta cgttacgccc accgacctta cgctcttcct
     4021 tcgtcagcca gccctctttc gccagttctg caatagcaga tttaacggtg gattcacttc
     4081 ttgcaccgat ctgacgccgg atagtttcaa tggcaggcca tgacacgccc tcgtcattgc
     4141 tgtagtctgc aagacgggcc ataaccgcca ccctggataa gatcatgccg gtgaaggcgc
     4201 acccttccca gacaagacca tgaagcttgc tgctcataaa ccccccgaac accgtgcttt
     4261 tagtgcatca ccacagcatt ccctgccggg ccgccgcgat tcatctggtc atacaaaaca
     4321 accgctgacg caacaaaatc atcgacatcc ttcaccagcc gatccctccg ttcgacgatc
     4381 tcacggtaat attcagaact gtggctgcgc atacgggcca ccagcaaagg cggcatcgcc
     4441 ttttcgatcg ccggtaacag agcctgcatt ttttcaacag catcaggggt gtctttatcc
     4501 agccaacgga aaattttctg tgtattacgg gccagggctt ccggatggct gtcgtcgtac
     4561 agttcaggga acgtcatccc cagttcgaaa taagtccggg ctatttcagc tgcaggaact
     4621 ttctcaccgt ccggataggc ccaggcattg atcgccatgc ggatgtgctc atgcttgatt
     4681 ttcatgaatc aagctcctag aaagtggttg tgttaacgtt ttggtatctt ccagctcagg
     4741 ccaaatattc atccaatcaa aaggccttag ttgctgacgt gtaacttcac cattactggc
     4801 tcgctcaata aggacacata acgatgcccc taacacttgg cctttactca atgcctttct
     4861 tagataaccg atgctggtac cacactcgca tgcaaacata cgctgttcat ctgacgaaag
     4921 agaattgaga aatattctta attcttccat agctactcct tagtaaacac agcaaagaat
     4981 acctataggt aaacaaaagt caataccttt gggttgttta ccttacggta atcacatcta
     5041 ttatttacct atggacaaat atgaatttag acgacagcag ctcatcaaaa ttcgtgatga
     5101 gaaatgcgat ggtaaagcgg ttaacgtagc cagaaagatc ggtcgcgagc cttcttatgt
     5161 atcaagaatg ttgtacccag aggggaaaaa gggaaaaaaa cggatcgctg atgatatggt
     5221 ggagattatc gaagagtcct ttgggttacc ccgaggatgg atggatggta tcgtttcatc
     5281 atcaacgaac acagcctcca attatgaaac aagggttcta acgccacgac aacgtatttt
     5341 tttagatctc ttagacgagc tgccagaaag tgaagcggat aacttattaa aaactcttga
     5401 agagaaaaaa cagtattaca atatgatcta cgaagaaatc cgtaaaaaga aagcacaaaa
     5461 cgcatcataa ctcaccaaac aaccagtcac cagttaagac gcctgaaaaa aagttaccca
     5521 tgggtattta ctttttgggt acccatgggt atgctccctt tcataccaac ccactccgcc
     5581 ccacagaatg cagggcaata cctcgagtta cccggcagtg gtcaggggtt aagtagccag
     5641 cccgaggcgt atgaacatga cggcgggaac actttgtata acagcgcagc aggtttttag
     5701 ttccgctacc cggcgttaag ggtaaatggg gtcaacatgg atactatcga gcttggcaac
     5761 aacgaatctc tggtgtgcgg agtattcccc aaccaagacg gcacgtttac cgcgatgacg
     5821 tataccaaaa gtaaaacgtt taaaaccgaa gctggcgcgc atcgctggtt agccagaaac
     5881 gctaactgat tagcgccagt aaaaacaggt ttccacaggt taatttaccc tgaaaagtca
     5941 gggcataaca cgaaagcgca cggcgagatt cctttgcata taaggttaga tttcttcgac
     6001 cgtgcgcttc cggttgtggc aatccgcgaa atggcgcggc ggtaagtatg gcggggttat
     6061 tccttccccc gttgaggaca ccgggttgtc aggttgacca tacgcttaag tgacacccca
     6121 gccacaacaa cctctgttat cacttttctg gtgattcggc ggaaatgaat atccgccctt
     6181 tttaaagtga attttgtgat gctgtgaatg cggctatgcg cacgcggaac agttaaagca
     6241 gtaaggcggt cttttactgg cgtaacgagc atcaactaac ccgacgttaa ttgttaactg
     6301 gttaacgtca cctggaggca ccaggcgctg catcacaaaa ttcattgttg aggacgcgat
     6361 aatggaaacg ttattaccaa acgttaatac gtctgaaggt tgttttgata ttggtgttct
     6421 gctcagtaat aaagcgttta ctgaggatgc cattaatatg cggaaatatg agccttatct
     6481 gctcaatgat aattccattc tttcccgaat tgcccttatt aaacttggta ttttcggaga
     6541 acgtcaatga cttcagcatt tgcactggtg atgacggttt ttcttataac gggtgaataa
     6601 cagaatgtga ttaccggaat ttatgccagt aaagaatcct gcctccaggc aagagacgag
     6661 caaaaaattt ctggtgaatg cctaccggta aaaaaagtat cgctgaacct gaataacgaa
     6721 acaccggctg gataacccgc cagccatatt aacgccatac caacggatta aaaatgccag
     6781 caatagcagg gattcgttca ccctgaaatc tgtaatgagg ttaaaacaca atgaataaaa
     6841 tctttatttg cgctgctatt cctgacgaac aggccataga agaagatagc gctgttgcgg
     6901 tggccactgc cattgaagcc ggtgatgagc gtcgcgcacg cgcaaaattt cactggcaat
     6961 ttctggagca attcccggca gctcaggact gcgcttataa atttattgtc tgcgagg
//
LOCUS       DAZFQD010000200         6776 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.200, whole genome shotgun sequence.
ACCESSION   DAZFQD010000200 DAZFQD010000000
VERSION     DAZFQD010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6776
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.200"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(239..724)
                     /gene="sixA"
                     /locus_tag="ACHKTW_004812"
     CDS             complement(239..724)
                     /gene="sixA"
                     /locus_tag="ACHKTW_004812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HFO6465689.1"
                     /translation="MQVFIMRHGDAALDAASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
     gene            complement(930..3071)
                     /gene="fadJ"
                     /locus_tag="ACHKTW_004813"
     CDS             complement(930..3071)
                     /gene="fadJ"
                     /locus_tag="ACHKTW_004813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HFO6465690.1"
                     /translation="MEMASVFTLNVRLDNIAIITIDVPGEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFVSAKPDNFIAGADINMIGNCKTAQEAEALARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPHSILLEVAVELAKKDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTEVKKDPGSDAPPALLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISVTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHATRPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMGARGESFWKTTATDL"
     gene            complement(3071..4381)
                     /gene="fadI"
                     /locus_tag="ACHKTW_004814"
     CDS             complement(3071..4381)
                     /gene="fadI"
                     /locus_tag="ACHKTW_004814"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HFO6465691.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLARVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SDLTLIDMHEAFAAQTLANIQLLGSERFAREVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            complement(4561..4845)
                     /locus_tag="ACHKTW_004815"
     CDS             complement(4561..4845)
                     /locus_tag="ACHKTW_004815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HFO6465692.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKARSVESEPCKISPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            5217..6557
                     /gene="fadL"
                     /locus_tag="ACHKTW_004816"
     CDS             5217..6557
                     /gene="fadL"
                     /locus_tag="ACHKTW_004816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HFO6465693.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAVYARAKIERFAGDLGQLVAGQIMQSPAGQTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
BASE COUNT         1696 a         1820 c         1609 g         1651 t
ORIGIN      
        1 ttctctatta tctgtattaa taaatgacaa tgagtgtcaa acagacatta ttcatttcaa
       61 catgtaactt tgggcaaaat caagatacgc gcagaagaat aatttccgaa tgaatgtaga
      121 aagtgtttta tttatagata aggagagtct tcgatagtag tgaggttgac agatatccgc
      181 tgcctgatga gcaattatgc ccaatgaatc tacctcattg ggcataattt ggaacacatc
      241 agatagcttt tgccatcttc agattacacg ggctcatctg ccagttgaat gtaccgtttc
      301 cgctttcatc aagcgttacg ctggcaatgg ccgaggtggt aaacataggc ggcgtttcgc
      361 ccggacataa ctcggcaacc agatacccga ctaacggtaa gtgagagatc accagtaccg
      421 aagcaacacc ttcattggtc aacgcctgca aataggcact gaccagacca acatcgccgc
      481 agggcgttaa ctccggcaga acttctgcac tggaaggcag gttcaaacaa tcccctactt
      541 cttccagtgt ttgctcggct cgcaggaacg ggctcaccag aacgcgttcg atttccactt
      601 tttgaccttt cagccagttc gccatcaggc gagattcgtc acaaccatta gtggtcagag
      661 gacgaacgga atcactggcg gcatcgaggg ctgcgtcgcc gtgacgcatg ataaaaactt
      721 gcatattgca ccgcttttgt taaccagatt cgcccgtttt ctttcatctg aaaccagaga
      781 aagaaaacgg tggccggcat tgtgccttat ccattcaccg aatgaaacgc tgttttttac
      841 ctcaatggcg taagtatagt caatatgcgt ttacattttg accaacactc cgccattcag
      901 cgcggattca tatagctttg accttcttat tacaggtcag ttgcagttgt tttccaaaaa
      961 ctttccccac gcgcgcccat ctcgaccaaa cgctcgcaag gggtaaaacg ggaaccatac
     1021 tgcgtggcaa gtcgttgcat tattgcaacc acttcgcccg cgccgagaga atcgatatag
     1081 cggaacggtc caccgagaaa tggcggaaaa ccaatgccaa ataccgcgcc aatatccccg
     1141 tcacgcacgc tacggataac ctgctcatca acacaacgta ctgcttcatt cagcatcaac
     1201 atcacacacc gttcagcaac ctgcggtgcg gagagtcgcc cctgcccttg tgcgccaatg
     1261 agcgggtaaa tggcaggatc gacctgtttt ttgcttttac gccctttctg accataaaga
     1321 tagaaacccc gaccattttt tctgcctttg cgatcgtcgt tcaaaattga agaaacaaca
     1381 tttgcaggcg cgctaaaacg ttctccataa gcggcttcca gtacaggaat aattttagtc
     1441 ccggtgtcga ttcctacctc atccaaaagt tggattgggc ctaccggaaa accaaatttc
     1501 actagcgcag catcaatgtg ctctacccgt tcaccttcgg tcagcatgcg gatagcttca
     1561 ttaatgtaag gcgctaagat gcgattgacg taaaacccgg ctttgtcacg cacgacaatg
     1621 ggcgttttgc cctgtttttt cgccagtttt accgtagtgg cgatggtttg cgctgatgta
     1681 cccgcatgag gaataatctc caccagcggc attttttcca ccggactgaa gaaatgcagg
     1741 ccgataactt gctcaggtcg cgtggcgtga gcggcgatat caccaatcgg caaagatgac
     1801 gtattcgaag caaagatggt atgagcggcg caattttgct ccacttccgc caccatctgt
     1861 tgttttaatt cgagattttc aaacaccgct tcaataatca gatcgcgatg ggcaaagccg
     1921 cgatagtccg tcgttacgga gattaatgcc agctgtttgt cacgttcgct ggctttgaga
     1981 tgacgacggc gaactttgcc ctccagctga tcccaactgt acttcagcgc atgatttatg
     2041 ccctgcgggt tgatatcttt aattctgacc ggaagccccg ctttacaagc agtgacataa
     2101 gcaataccgc cgcccatcaa gccaccacct aaaattccca cgctgtttaa tagcgcaggc
     2161 ggtgcatcac taccgggatc tttcttcact tccgtactgg caaaaaagat actacgcaaa
     2221 gcttgcgatt gtggcgtcat cgccagttcg ccaaacgccc gagcttcggc gtcataaccg
     2281 ctgctggtgc cctgcgctaa tcccgtttca acaacctcca ggatgcgttc tgtcgccgga
     2341 taattgcctt gagttttgtg ttctgttttc ttgccgacca ttttgaacag cagcgcacga
     2401 cctaacggcc ccgccagaat acgctcgcgt acaggtagag ggcgggaaga tgggcgatcc
     2461 ttcttcgcca gctcaacagc gacttccagc aaaatggagt gcggaacaac gtcatccacc
     2521 agccccagct ttaatgcctg tttcgcccga agttgttttc cggtgaggat catctctaat
     2581 gctgtgctga cgcctatcag acgcggtaaa cgctgggtgc cgcctgaacc gggtaacaat
     2641 ccaagttgta cttcaggcaa accgagcacc gttttaggat cgtcagtaca aacgcgaccg
     2701 tggcacgcca gcgccaactc cagcccacca cccaggcaag cgccatgaat agccgcgata
     2761 accggaatgg gcaaagcatg aatctccgcc atcaactgtt ggccctgccg cgccagtgct
     2821 tccgcttctt gcgccgtttt gcagttgccg atcatgttga tgtctgcgcc agcaatgaag
     2881 ttgtccggtt tagcggagac aaacaccacg cctcgcaact ctttgttttc acggagttgc
     2941 ttaataatgg cgcgcacctg cgaggcaaac tccgccttca gggtattcat tttctcaccc
     3001 ggtacgtcga tggtgatgat agcaatgttg tccagacgaa cattaagagt aaacactgat
     3061 gccatttcca ttattccgcc tccagaacca ttgccgcgcc aagcccaccg gcagcacagg
     3121 cggtaactaa accaaatcca ccgccgcgac ggcgaagttc atgcaatgtc tgggtaatca
     3181 tccgcgcgcc ggtcgccgcg aagggatgcc cgtaagcaat cgaaccgcca agcacgttaa
     3241 atttgctatc gtccacttcg ccagtggcat gtgcacgccc cagtacttca cgagcaaaac
     3301 gttcactacc cagcaactga atattcgcca gtgtctgagc tgcaaaggct tcgtgcatat
     3361 cgatcaatgt cagatcgctc attgtcaaac cggcacgttc cagcgccagc ggtgttgacc
     3421 aggccggacc gagcaacatg tcctgccaga catcaatcgc agtaaatgcg tagctgcgca
     3481 gataccccag cggcaccagc cctaattctt tcgcccggga ttcagtcatc aggatcaccg
     3541 ccgctgcgcc atcggtcagc ggcgtactgt tcgccgccgt taccgttccg tgtttgcgat
     3601 caaacgccgg gcgcagcttt gcgtaatcgg caagcgagga attaccgcga atattgttgt
     3661 cttcgacaag cggttgttta taaggaggga taaaggcagt catcacctct tctttgagtt
     3721 taccgtctga ccacgcctga gcggcacgct gatgcgaacg gtgcgctaat gcatcttgct
     3781 gttctcgggt gatgccgtag gttttcgcca tttgttcggc ggtgtcgccc atccgcaagc
     3841 cagtagaata ttctgctacc gcaggcggta cgggcattaa gtcgcgcaaa cgcaggcgag
     3901 agaagagttt cagtcgctgg ctcatggttc gagctttgtt gacatcaacc agcacgcgcg
     3961 ccagtttttt actgacgcca attggcaata cggaagagga atctgcccca ccggcaatcc
     4021 ccgctcggat agttcccgcc atcaggcttt ctgcgacgtt tgcaaccgcc tggaaactgg
     4081 tagcgcaagc gcggctgacg ctgtaagcat cggtatgcac attcattccg gtaccgagaa
     4141 caatttcacg cgcaatgttg ggggcttcag gcatttgtac gacctgacca aagaccagct
     4201 gttcaatcac ttcagcgggg atctcgctgc gtgccagcag ttcgcctacc accatcttcc
     4261 ctaaatcaac cgcgggaatg ccatgaaaag ccgtcgcctg gcgggcaaaa ggcgtacgta
     4321 aaccgctaac aatggcgata cgatcgccct ggcgggtaac cagcggtaaa acctgaccca
     4381 taacactccc ctgtaaaaaa ataaataaag tggtctgacc tgatcatagt cttaaccatt
     4441 tttttacatt tagccaagcg gagaaaaagg aaagtgggag ctatgacaca gtgaagacag
     4501 gagaaagaaa agaaaacgcc cctgccagca actgacaggg gcgaacgtac ccgagaggaa
     4561 ttaacgcaga cccagctgga agatcagcat ttcagcttcg caggcaaaag tgaaatcgat
     4621 atccaggcgc acaccgtcag actcttcagt gaaagtcggg gagattttgc acggttcgga
     4681 ttccacgcta cgggcttttt cagtcagcgc cgccagcgtt tgttctgctt ctgcgcggtt
     4741 tgcaaacacg cggctgtaag acgcggtgca gtcggagttg tccataatgg tgccaacatc
     4801 catacagcag caaaccgggg tttcatcagc actacattta ctcatcgttg atttcctctg
     4861 tatgtgcacc caaggtgcca gataaacgtt gtggatattt tacgcttccg gaaagtgctg
     4921 ctccagttgt taattctgca aaatcggata agtgaccgaa atcacactta aaaatgatct
     4981 aaaacaaaat tcacccgaat ccgcgagtgc gcaacctcca aattttgcca gctggatcgc
     5041 gtttcttaga tcatatttga aaaaagatgg aaacatactt gcaacattcc agctggtccg
     5101 acctatactc tcgccactgg tctgatttct aagatgtacc tcagacccta cacttcgcgc
     5161 tcctgttaca gcacgtaaca tagtttgtat aaaaataaat cattgaggtt atggtcatga
     5221 gccagaaaac cctgtttaca aagtctgctc tcgcagtcgc agtggcactt atctccaccc
     5281 aggcctggtc ggcaggcttt cagttaaacg aattttcttc ctctggcctg ggccgggctt
     5341 attcagggga aggcgcaatt gccgatgatg caggtaacgt tagccgtaac cccgcattga
     5401 ttaccatgtt tgaccgcccg acattttctg cgggtgcggt ttatattgac ccggatgtga
     5461 atatcagcgg aacgtctccg tctggtcgta gcctgaaagc cgataacatc gcgcctacgg
     5521 catgggttcc gaacatgcac tttgttgcac cgattaacga ccaatttggt tggggcgctt
     5581 ctattacctc taactatggt ctggcaacag agtttaatga tacttatgca ggcggctctg
     5641 tcgggggtac aactgacctt gaaaccatga acctgaactt aagcggtgcg tatcgcttaa
     5701 ataatgcatg gagctttggt cttggtttca acgccgtcta cgctcgcgcg aaaattgaac
     5761 gtttcgcagg cgatctgggg cagttggttg ctggtcagat tatgcaatct cctgccgggc
     5821 aaactcctca agggcaagca ttggcagcta ccgccaacgg tatcgacagt aataccaaaa
     5881 tcgctcatct gaacggtaac cagtggggct ttggctggaa cgccggaatc ctgtatgaac
     5941 tggataaaaa taaccgctat gcactgacct accgttctga agtgaaaatt gacttcaaag
     6001 gtaactacag cagcgatctt aatcgtgcgt ttaataacta cggtttgcca attcctaccg
     6061 cgacaggtgg cgcaacgcaa tcgggttatc tgacgctgaa cctgcctgaa atgtgggaag
     6121 tgtcaggtta taaccgtgtt gatccacagt gggcgattca ctatagcctg gcttacacca
     6181 gctggagtca gttccagcag ctgaaagcga cctcaaccag tggcgacacg ctgttccaga
     6241 aacatgaagg ctttaaagat gcttaccgca tcgcgttggg taccacttat tactacgatg
     6301 ataactggac cttccgtacc ggtatcgcct ttgatgacag cccagttccg gctcagaatc
     6361 gttctatctc cattccggac caggaccgtt tctggctgag tgcaggtacg acttacgcat
     6421 ttaataaaga tgcttcagtc gacgttggtg tttcttatat gcacggtcag agcgtgaaaa
     6481 ttaacgaagg cccataccag ttcgagtctg aaggtaaagc ctggctgttc ggtactaact
     6541 ttaactacgc gttctgataa cacgttcgcc tggataaagt cacctgcata gcaggtgact
     6601 ttaactcccc cacttcaccg aaagtagtgt cctcattgct tacctcctga gttttgcaaa
     6661 cagcctgatg gcagcttgcc ctttcaaatc aataagcggt atccacagac aaatctgaag
     6721 ggaaaggcat attttcaggc gttctgctcg tccttctcaa agagtttact tttctg
//
LOCUS       DAZFQD010000201         6672 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.201, whole genome shotgun sequence.
ACCESSION   DAZFQD010000201 DAZFQD010000000
VERSION     DAZFQD010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6672
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.201"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            78..779
                     /locus_tag="ACHKTW_004817"
     CDS             78..779
                     /locus_tag="ACHKTW_004817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFO6465694.1"
                     /translation="MSDLIAYKSNALVEASYKLTLQEQRFLLLCISRLKSGADAASSE
                     LQKNLIITAEEYFNSFPDMGRKNAEIQLQEAVDRLWDRSILLKDDEKREEFRWIQRRA
                     QYLKGEAKVQITFSDSVMPYLTQLQGQFTKVIIKNISRLSRSYSIRIYELLQQFRSTG
                     ERIISLDDFRSMLDICDKYQTYKSLNQQLLKPCIDELNLKSDLLVTVETIKKGRTVVA
                     LHFRFKEDKQIKIPS"
     gene            1140..2258
                     /locus_tag="ACHKTW_004818"
     CDS             1140..2258
                     /locus_tag="ACHKTW_004818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO6465695.1"
                     /translation="MAKLYFYLVLAASLSALSTDMIVPALPELQKAFNADYSLIQLSI
                     SGFLIVFAFSQLISGTIGDYFGKVRVMTICLIIFLIGSIVCFMANNLNMLLLGRILQA
                     IGAGAGPVISKAIAKESFPPLKLKRALSDISSTSAIIPLIAPLAGALILDYYNWNVIF
                     IVMGAFSILTILLSPKESNKQEKKTHQDNVKFVTKDFICGTILVSLMLSSLFCYISIS
                     PALFMSDYGLNTFNYSVVFSLSVLFFIIGNQLSKIEKISNPWILFPLNAISVTPFLIL
                     NDAFIICVIGAMIFNLTLGAYYPIANFIALQINGSRTGLAASITGFTQTVSAGTISFL
                     SVKISDNGVSLGTTLSISCLILALLSIFVTIIIGNNKK"
     gene            2255..3478
                     /locus_tag="ACHKTW_004819"
     CDS             2255..3478
                     /locus_tag="ACHKTW_004819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001104444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFO6465696.1"
                     /translation="MNWQKKKIERSIKYQTRVFNEHINELVITRRNGKVITVNDGRDM
                     IEFVSCSYLGLETHENLKHAIANAIERFGVQLSVARTRVKVDLFEQLEDRLNKIMNGA
                     HTLTFNAVTPCHLAVLPLLASGTLPNYKFNKIPYFILEKTAHATLQINRGLLQQFGEI
                     IRIDFQHKNDIEKAFKYASINNMVPISISDSVGSMGGVAPVEFIVRMANRFDGYAYID
                     DAHGTSIYGKNGSGYVMSCLDNKLDDRIIIAGSLSKAFGSHGGFIACNYPDTINFIKT
                     YGTTYAFGGPPSLPGIAACIAAADLHLDGTVSSRQNELQQVIKYFDDKFNETEVINKG
                     TTMPIRGLLIGNEEKAITICKKMHDRGFATTVAMYPTVEEGKAILRCAISAIHTRENI
                     DSLHKNFIECINELG"
     gene            complement(3548..5764)
                     /gene="mobQ"
                     /locus_tag="ACHKTW_004820"
     CDS             complement(3548..5764)
                     /gene="mobQ"
                     /locus_tag="ACHKTW_004820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="HFO6465697.1"
                     /translation="MAIYHLSMKIISRKNGYSAVASAAYRSGSVIPDDRTGLTHDYTR
                     KRGVDDAVILTPENAPSWCADRSVLWNAVEKAEQRRDSQLAREIELAIPREISRDVAR
                     ETVLAFVRENFVSQGMIADVAFHHMDRTNPHAHIMLTTRAVGETGFSGKVRDWNDRTL
                     AETWRASWADHANRALANAGYLEEIDHRSYERQGLEKAPGLHLGKAACAMEKRGMETE
                     RGEQNRLINSLNLEIQVSRTQLALRKVQETQRKRELSDAARRAAEALNLTIPAANASA
                     DTLREFIATLPQECGNAWEMTPEFVAMNGKANDIKREGNALVKERDILEKEMTGLRKA
                     HPVASILSELPLMTWAEPEYRKRQLRFWKLEKQIESLRRTYRAVKERDIPARRQAFET
                     QWNTWISPGMAELKEKLSAREAERHREEAEAEARRKEQEYDARLKHDNHRLSRETALA
                     GVITGLGRAREPGTGMITRYMILSNRAGEFTVWGDELADYPQSVHDQVNVYLSPGGAV
                     MVTDIHSGLSESHETLARPERVRMYSCATVRHVLEQMRQGWPSYGFPALPHHWPDNFY
                     FSDDRRPAASPLPSAHRVDVTAYAAPEQLMPVVFSTERNSRTLNLLLCKGPEEMLVGF
                     VRLEDELRPVLALPSPDYSHLMVSIITENGIHLAGYGEAINRDADTPYPPEPKLMQFR
                     LKGCQDTLFAAINKPEEMPDYLFRQLGFNQTWHEWKRDEQHRQQQRRPGHYRGMSM"
     gene            5943..6221
                     /locus_tag="ACHKTW_004821"
     CDS             5943..6221
                     /locus_tag="ACHKTW_004821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="HFO6465698.1"
                     /translation="MSRTLEQKIAEAEARLQRLKAKSRSLDTAQKVIVGAAMLARVRR
                     PEEAQLRAFLLQFLRKEVTRQADVNRIQPLINELEKLPGPPVKPQKNG"
BASE COUNT         1838 a         1470 c         1478 g         1886 t
ORIGIN      
        1 aaaaaaaaaa tatatattgc cttttcgtat tggttatgtt tattttttgt gtattgcctt
       61 tttctcggag atgcactatg tccgatctga ttgcctataa atccaatgcg ttagttgagg
      121 ccagttataa gctcacctta caagaacaac gtttcttgtt gctttgtatc agtcgattga
      181 aatcaggagc tgatgcagca tcatcggagt tgcaaaaaaa tctgataatt acggcggagg
      241 agtatttcaa ttcattccct gatatgggca gaaaaaacgc tgagattcag ttacaggaag
      301 ctgttgatcg gctgtgggac agatcgatcc ttctcaagga tgacgagaag cgagaggaat
      361 ttcgttggat acaacgtcgt gcgcaatacc tgaaaggcga agcaaaggta cagatcactt
      421 tttctgattc tgtgatgcca tacctaacgc aactacaagg gcaattcaca aaggtgataa
      481 ttaagaatat atcaagatta tcaagaagtt acagcattag gatttacgag ttgctccagc
      541 agtttcgcag tacaggtgag cgcattattt cacttgatga ctttcgttca atgcttgata
      601 tttgtgataa atatcaaaca tataaatctc ttaaccaaca gttattaaaa ccctgcattg
      661 atgaattaaa cctgaaaagt gatctgcttg ttactgttga gacaatcaag aaggggcgta
      721 cggttgtagc actacatttt cgcttcaagg aagataaaca gatcaaaatc ccctcataaa
      781 ccgacctaaa acgcaatctg acgcaaaaaa gtggatatgg acgtaaacac cctcacccaa
      841 ataagaaaac gcgtcagaat cgttttcagg ggcctgcata actatgcacg aaagcgatgc
      901 tcgatcggtt tcgaccgttc cgaagggcga acaggtcaaa atgacaagtt ttctcccacc
      961 ggatggaaga ttctgcacgg ggatttttta tccccttcca tcaacctgaa tatgcatctg
     1021 ggagtaccca ttagtttctg atcaaatact atcgcagtgc tatcttaaaa tataaaacat
     1081 ttcaatggtg taagaatttt aaatatgata aataaagata tttttatcag gggttaatta
     1141 tggcaaaatt atatttttac cttgttcttg ctgcatcatt atcggcatta tcaactgaca
     1201 tgattgtgcc tgcgttacca gaactacaga aagcatttaa tgctgactac tcattaattc
     1261 aactaagtat tagtggattt cttattgtat ttgcatttag tcaactgata tcaggaacca
     1321 taggtgatta ttttggaaaa gtacgcgtaa tgactatatg cttaattatt tttcttattg
     1381 gtagtattgt atgttttatg gccaataatt taaatatgct tcttttgggt agaattctac
     1441 aggcaatagg tgcaggtgcc ggacctgtaa tatcaaaagc aattgcaaaa gagtcttttc
     1501 caccactaaa gctaaaaaga gcactttctg acatatcatc aacaagtgcg ataataccat
     1561 taatagcacc tctggccgga gcattaatac ttgattatta taactggaat gtaatattta
     1621 ttgtaatggg tgcatttagc attctaacca tactcctgtc acctaaagag tctaataaac
     1681 aggagaaaaa aacacatcaa gataatgtaa aatttgtgac taaagatttc atctgcggga
     1741 caatactcgt ttcactaatg ctaagctcac tattttgcta catatcaata tcacctgcac
     1801 tatttatgag cgattatgga ttaaatacat ttaactactc tgttgttttt tccctgtcag
     1861 ttttgttttt catcattgga aaccaattat caaaaatcga aaaaatatca aacccatgga
     1921 tcttatttcc cctaaacgca atatcagtca caccttttct tatattaaat gatgctttta
     1981 taatatgtgt tattggagca atgattttca atttaactct tggcgcatac tatccaatag
     2041 ctaatttcat cgcattacag ataaacggtt cacgaactgg tttggcagca tcaattacag
     2101 gctttactca aacagtaagc gcagggacaa ttagtttcct ttcagtaaaa ataagcgata
     2161 atggagtttc tttgggaacc actttaagta ttagctgtct tattcttgca ttattgtcaa
     2221 tattcgtaac cattattata gggaataata aaaaatgaat tggcaaaaga aaaagataga
     2281 gcgttcaatc aaataccaaa caagagtatt taatgaacat ataaatgaac tagtgatcac
     2341 cagaagaaat ggtaaggtaa tcactgttaa tgatgggagg gatatgattg agtttgtgtc
     2401 atgctcttat cttggactgg aaacacatga aaatctaaaa catgcaatcg ctaatgcaat
     2461 tgaaagattt ggagtccaac tttcggtcgc cagaacgcgc gtaaaagtag atttattcga
     2521 gcaactggaa gatagattaa ataaaataat gaatggggcg catacattaa catttaatgc
     2581 ggttactcca tgccatttag ctgtactccc acttcttgca tcaggaacac tacctaatta
     2641 caagtttaat aaaataccat attttatttt agagaaaaca gcgcatgcaa cattgcaaat
     2701 aaatagaggg ctattacaac agttcggtga aataatcagg attgactttc aacataaaaa
     2761 tgatatagaa aaagcgttta aatatgcttc aataaataac atggtaccaa tatctatatc
     2821 tgatagtgtc ggttcgatgg gtggggtagc tcctgtcgaa tttattgtta gaatggctaa
     2881 cagatttgat gggtatgctt acattgatga tgctcatggc acgtcaatat atggaaaaaa
     2941 tggctccgga tatgtaatgt cttgtctgga taacaaactt gatgacagaa ttattatcgc
     3001 tggttcatta tctaaagcgt ttggttctca tggtggcttt attgcatgca attatcctga
     3061 caccataaac tttataaaaa cgtatggaac tacctatgct tttggtgggc caccatcatt
     3121 accaggaatt gctgcatgta tcgcagcagc agatttacac ctcgatggca cagtatcctc
     3181 gcgtcaaaat gaattacaac aagttataaa atactttgat gacaaattta acgaaaccga
     3241 agtcataaac aaagggacta caatgccaat acgtggattg ttaattggca atgaagaaaa
     3301 agctataacg atatgcaaaa aaatgcatga tcgtggtttt gctacaaccg ttgctatgta
     3361 tcctacagtg gaagagggaa aggcgattct acgttgtgca atatcagcta ttcatacccg
     3421 agaaaatata gatagcctac ataaaaactt tatagaatgt attaacgaac tgggttagta
     3481 aatatcatgg cctctttata gagaggccat ctatttcatt ttttatggcg tatagctgtc
     3541 cctgacatta catactcatg ccccggtagt gtccggggcg tcgttgttgt tgcctgtgct
     3601 gttcgtcccg cttccattca tgccaggtct gattaaatcc gagttgacgg aagagataat
     3661 ccggcatctc ttccggtttg ttgatggcag caaaaagcgt gtcctgacag cccttaagcc
     3721 tgaactgcat cagcttcggt tccggtgggt acggagtatc agcatcacgg tttatggcct
     3781 caccgtagcc tgccaggtgt atcccgttct ctgtgatgat gcttaccatc agatgactgt
     3841 aatccggcga cggaagcgca agaacgggac gcagctcgtc ctccaggcgc acaaaaccga
     3901 caagcatttc ctccggccct ttgcacaaca gcagattcag cgtcctgctg tttcgctctg
     3961 tcgaaaagac aacgggcatg agttgctccg gtgccgcata agcggtgacg tccacccggt
     4021 gcgctgacgg cagcggagag gctgcgggcc tgcggtcatc gctgaaataa aaattatccg
     4081 gccagtgatg cggcagcgcc ggaaaaccgt aagagggcca cccctggcgc atctgttcca
     4141 gtacatgccg gaccgtcgca caggaataca ttctcacacg ctcaggccgg gccagcgtct
     4201 catgagattc tgacagcccc gaatgtatat ccgtgaccat cacggcccca cctggcgaca
     4261 gataaacatt cacctggtca tgaacactct ggggataatc cgccagttca tcaccccata
     4321 ccgtgaattc tccggctctg ttactcagta tcatgtagcg ggttatcatg cccgttcccg
     4381 gctctctggc acgccccagc cccgtaataa ccccggctaa tgccgtttca cggctcaggc
     4441 gatggttatc gtgtttcagc cgtgcgtcat attcctgttc cttccggcgc gcttccgctt
     4501 cggcttcctc cctgtgccgc tccgcttccc gtgctgacag tttttctttc agctctgcca
     4561 ttcccggcga aatccacgta ttccactgcg tttcaaaggc ctgacgacgg gcgggaatgt
     4621 cccgttcttt cacggccctg taggtacggc gaagagattc aatctgtttc tctagtttcc
     4681 agaagcggag ttgtcttttg cggtattccg gttcagccca tgtcatcagg ggaagctctg
     4741 acaggatgga cgctaccgga tgggcttttc tgagtccggt catctctttt tcgagaatat
     4801 cccgctcttt caccagcgca ttcccctcac gtttgatgtc gtttgccttc ccgttcatcg
     4861 ccacgaactc cggtgtcatc tcccatgcgt tcccgcattc ctgcggcagt gtggcaatga
     4921 attcccgcag ggtatccgct gaggcattcg cggcgggaat ggtcagatta agggcttccg
     4981 ctgcgcgacg tgcggcatcg ctgagttccc gcttacgctg cgtttcctga accttcctga
     5041 gcgccagctg cgtgcgggaa acctgtattt ccaggttaag gctgttaatc aggcggttct
     5101 gctcaccgcg ttctgtttcc attccccgtt tctccatcgc acaggcagcc tttccgaggt
     5161 gaaggcccgg cgctttctcc agtccctgac gctcgtatga acgatggtct atctcttcca
     5221 ggtaaccggc gttcgcaagc gctctgttcg catggtcagc ccatgacgcg cgccacgtct
     5281 cggctagtgt ccggtcgttc cagtccctga cctttcctga gaatcccgtt tccccgacag
     5341 ctctcgtggt cagcatgatg tgcgcatggg gattggtccg gtccatgtga tggaacgcca
     5401 catcggcaat catgccctgg ctgacaaagt tttccctgac aaaagcgaga acggtttccc
     5461 gtgcgacatc gcgggaaatc tctcggggaa tggcaagttc aatctccctt gccagctggg
     5521 agtcccggcg ctgttcggct ttctcgaccg cattccagag aacggaacgg tcagcacacc
     5581 atgacggtgc attctcaggg gtgagaatga ccgcatcatc aacgccgcgt ttacgggtgt
     5641 aatcgtgggt taatccggta cggtcatcgg gtatgacaga gccggaacgg taggcagcag
     5701 aagcaacggc actgtaaccg ttttttcgcg aaatgatttt catgctgaga tgataaatcg
     5761 ccatttctgc cgtaatgcct ttttgccgtt ttcttttagc gtcgcagacc gcacacaatc
     5821 gaaacgaagt gaagattgta taagtgcgca cttcgtgatg tgccgtgcat tatgccagtt
     5881 cggttttgca actaccagtg acgcgctaaa atgcgatttt tcaccgcgca cggaatcaca
     5941 aaatgagcag aacactcgaa cagaagattg ccgaagctga ggccagattg cagcgcctga
     6001 aggcgaagag ccggagcctg gacacggcgc aaaaagtcat tgtgggggca gccatgctgg
     6061 caagggtacg gaggccggaa gaggcacaac tgcgtgcgtt cctgctccag ttcctgcgaa
     6121 aggaagtcac aagacaggcc gatgtgaacc ggatacagcc gctgattaac gagctggaga
     6181 agctacccgg accacctgta aaaccgcaaa agaacggttg atctctttcc gctcgccgca
     6241 gccccagcga ccgagcgtta gcgagtaagt gggcgaggaa gcggcagaag tgcagttgct
     6301 gaatgtggca catacgcact gttttcagaa aggtgatcct ctctgaaccc gcatgaatac
     6361 tggatttcca caggtttgca cgttgttttt gcagaacgcg gcaaactgat ttttttaacg
     6421 tattgttttt tttagtgttt ttttttgcgc tttgagtttt caacaggcac gccgttaagt
     6481 ttttaaatct tgtttttata tacttgtttt tatacttgtt tttaggtgcc gattttgcct
     6541 ttaaaatcat attattacag aggtaaaggt aatatgattt aaggtaaagg taatatgatt
     6601 taaggtgttt aggtaatatg atttaaggta aaggtaatat gatttaaggt gttatccaca
     6661 gaaataggga tt
//
LOCUS       DAZFQD010000202         6332 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.202, whole genome shotgun sequence.
ACCESSION   DAZFQD010000202 DAZFQD010000000
VERSION     DAZFQD010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6332
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.202"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..400
                     /locus_tag="ACHKTW_004822"
     CDS             <1..400
                     /locus_tag="ACHKTW_004822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6465699.1"
                     /translation="ILAREFFTPENRKSVPAHDESWVIICDALLGRLMCCMFQGRRYL
                     QIDAEDVTGRLETYRKIRNGEWVHNTYARPLTMSEMVVMFGYVYRESKPCHLAREMGI
                     NTKTVNTFLYMGLGKNGLKYRSVKHLVGRA"
     gene            complement(612..860)
                     /locus_tag="ACHKTW_004823"
     CDS             complement(612..860)
                     /locus_tag="ACHKTW_004823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFO6465700.1"
                     /translation="MRIEICIAKEKICKMPKGSVPALQQEMLRRVGKRYDDVDVIVKS
                     ASNDGLTILRAVDKESAQEFVQETLQEVWETADDWFIR"
     gene            complement(1095..1190)
                     /locus_tag="ACHKTW_004824"
                     /pseudo
     CDS             complement(1095..1190)
                     /locus_tag="ACHKTW_004824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310605.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1187..1753)
                     /gene="yecD"
                     /locus_tag="ACHKTW_004825"
     CDS             complement(1187..1753)
                     /gene="yecD"
                     /locus_tag="ACHKTW_004825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFO6465701.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVDAPSPAKVLPENWWQHPAALGATDSDIEIIKR
                     QWGAFYGTDLELQLRRRGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAAS
                     AEQHNNSINHIYPRIARVRSVEEILHAL"
     gene            2063..3835
                     /gene="aspS"
                     /locus_tag="ACHKTW_004826"
     CDS             2063..3835
                     /gene="aspS"
                     /locus_tag="ACHKTW_004826"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFO6465702.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADAMGALRLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTALAELSIQVVKKAENN"
     gene            3953..4405
                     /gene="nudB"
                     /locus_tag="ACHKTW_004827"
     CDS             3953..4405
                     /gene="nudB"
                     /locus_tag="ACHKTW_004827"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008828 - dATP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0019177 - dihydroneopterin triphosphate
                     pyrophosphohydrolase activity [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HFO6465703.1"
                     /translation="MEDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            4434..5174
                     /gene="yebC"
                     /locus_tag="ACHKTW_004828"
     CDS             4434..5174
                     /gene="yebC"
                     /locus_tag="ACHKTW_004828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFO6465704.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAVDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            5209..5730
                     /gene="ruvC"
                     /locus_tag="ACHKTW_004829"
     CDS             5209..5730
                     /gene="ruvC"
                     /locus_tag="ACHKTW_004829"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HFO6465705.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGIGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLARGRLR"
     gene            complement(5732..>6332)
                     /gene="yebB"
                     /locus_tag="ACHKTW_004830"
     CDS             complement(5732..>6332)
                     /gene="yebB"
                     /locus_tag="ACHKTW_004830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HFO6465706.1"
                     /translation="NINYPAEYEIGYIVFTCIGAALFGQISAASNCWSNHVGIIIGHN
                     GEDFLVAESRVPLSTITTLSRFIKRSSNQRYAIKRLDAGLTEQQKQRIVEQVPSRLRK
                     LYHTGFKYESSRQFCSKFVFDIYKEALCIPVGEIETFGELLNSNPNAKLTFWKFWFLG
                     SIPWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
BASE COUNT         1588 a         1521 c         1728 g         1495 t
ORIGIN      
        1 aatactggcg agggagtttt ttacaccaga gaacaggaag agtgtaccgg cacatgatga
       61 gtcatgggtg ataatctgtg atgccctcct tggcaggctg atgtgctgca tgtttcaggg
      121 acgccgttat cttcagattg atgcggaaga tgttacgggg cgcctggaga cgtacaggaa
      181 aatccggaat ggtgagtggg tacataatac gtatgcgcga cctctgacga tgtccgagat
      241 ggtggtgatg tttggatacg tgtaccggga gtcaaagccc tgccatcttg cccgtgaaat
      301 ggggataaac acgaagacgg tgaacacctt tctgtatatg ggacttggta aaaacggtct
      361 taagtacagg agtgtgaagc acctggttgg acgtgcgtag ccggaatgaa tattcgtgag
      421 tcgggtaatg agagttgaac ggtggcagtg agaatggagc aggaaaaaat cagatgcgga
      481 aagcggaaat acacagcggg tgcgatgtgc ttcatgctgt ttgtatggct gggtggtgtg
      541 gtgacactct gtgtacttgc tgcactgata acacaataaa agaggggagc tgccagctcc
      601 ccgtgctgat gtcaccggat aaaccagtca tccgctgttt cccagacttc ctgcagcgtt
      661 tcctgtacaa attcttgcgc ggattcttta tccacggctc ttaaaattgt gagtccatca
      721 ttactggcag atttcacaat cacatctaca tcatcataac gcttaccgac acgtcgtagc
      781 atttcctgtt gcaaggcagg aacagagcct tttggcattt tgcagatttt ttctttagcg
      841 atacagatct caatacgcat aacaccatcc tgtcactgtg cttatgtaca gtcgttattt
      901 ttatctgtat ggataaacag tgtcaatcat tcggattggt tatgggcata tatgggacgc
      961 tttggggcat gtatgggaca tttttaatcg gcgaaatccg ccgaagttcg tcgacatggt
     1021 aaacgaatca tctatccaac ccttgaaaaa cggcgctcct ggacgatctt cgtcgatttt
     1081 taaaaatgtt gcgtcacgca gttaaagtgg cgggcatact cttcaaggct ggtgatcccc
     1141 aaccgcaccc atttaggatg cgaccattgc ggtagaccga tgtaaatcat aacgcgtgaa
     1201 ggatctcttc cacactgcgc acacgggcga tgcgtgggta gatatggttg atgctgttat
     1261 tgtgctgctc ggcgctggcg gcactacagg cgtcttcggc aatcaccaga ttaaagccaa
     1321 gttcccaggc attgcgggcg gtggattcaa caccgatatt ggtcgagatc ccacataaca
     1381 ctattgtatc gatacctcgg cggcgcagtt gcaactccag atccgtaccg taaaacgcac
     1441 cccactgacg tttgatgatt tcgatatcac tgtcagttgc acctaatgca gctggatgtt
     1501 gccaccagtt ttcgggcaac actttagcgg gggagggggc atcaaccggc tgttttaatg
     1561 cttcggcgta atcggcagac cagccaacgc gcaccagaaa cacgggctga ccgctggcgc
     1621 gaaatttcgc cgccagcttc ccggcgcgat taactacctc atcggcagta tgtggaccac
     1681 cggcaaaagg taagatgcct tcttgtaaat cgatcaccac cagcgcagtg gttttagcat
     1741 taagttcaag cataatgact cctgaaaaaa caaaatcgtg cctcacacct taatgcgatt
     1801 agccgatggc ggaacgacaa attttgttaa tttttatgag ttcgggcaac acctttatag
     1861 caaactggcg tagagtctga tttcaggccc gatataaagt ggtaacgagg cgttccagcg
     1921 cgggccaggc tcttacggtg cggcagaatt tccagtataa tagccgcctt ttttcattca
     1981 gttgtgacat acagctaacg ctgcgacttg gtcacctgcg cagcaggggg catccgccgg
     2041 cagctaagtt aagggatatc tcatgcgtac agaatattgt ggacagctcc gtttgtccca
     2101 cgtggggcag caggtgactc tgtgtggttg ggtcaaccgt cgtcgtgatc ttggtagcct
     2161 gatcttcatc gatatgcgcg accgcgaagg tatcgtgcag gtatttttcg atccggatcg
     2221 tgcggacgcg ttaaagctgg cctctgaact gcgtaatgag ttctgcattc aggtcacggg
     2281 caccgtacgt gcgcgtgacg aaaaaaatat taaccgcgat atggcgaccg gcgaaatcga
     2341 agtgctggcg tcctcgctga ctatcatcaa ccgcgcagat gttctgccgc ttgactctaa
     2401 ccacgtcaac accgaagaag cgcgtctgaa ataccgctac ctcgacctgc gtcgcccgga
     2461 aatggctcag cgcctgaaaa cccgcgctaa aatcaccagc ctggtgcgcc gttttatgga
     2521 tgaccacggc ttcctcgaca tcgaaactcc gatgctgacc aaagccacgc cggaaggcgc
     2581 gcgtgactac ctggtgcctt ctcgtgtgca caaaggtaaa ttctacgcgc tgccacaatc
     2641 cccgcagttg ttcaaacagc tgctgatgat gtccggcttt gaccgctact atcagatcgt
     2701 taaatgcttc cgtgacgaag acctgcgcgc tgatcgtcag cctgaattta ctcagatcga
     2761 tgtggaaact tctttcatga ccgcgccgca agtgcgtgaa gtgatggaag cgctggtgcg
     2821 ccatctgtgg ctggaagtga agggcgtgga tctgggcgat ttcccggtaa tgacctttgc
     2881 ggaagcagaa cgccgttatg gttctgataa accggatctg cgtaacccga tggaactgac
     2941 cgacgttgct gatctgctga aatctgttga gtttgcagta tttgcaggtc cggcgaacga
     3001 tccgaaaggt cgcgttgccg ctctgcgcgt tccgggcggc gcatcgctga cccgtaagca
     3061 gatcgacgaa tacggtaact tcgttaaaat ctacggcgcg aaaggtctgg cttacatcaa
     3121 agttaacgaa cgcgcgaaag gtctggaagg tatcaacagc ccggtagcga agttccttaa
     3181 tgcagaaatc atcgaagcca tcctggatcg tactgccgcg caagatggcg atatgatttt
     3241 cttcggtgcc gacaacaaga aaattgttgc cgacgcgatg ggcgcactgc gcctgaaagt
     3301 gggtaaagac cttggtctga ccgacgaaag caaatgggca ccgctgtggg ttatcgactt
     3361 cccgatgttt gaagacgacg gtgaaggcgg cctgacggca atgcaccatc cgttcacctc
     3421 accgaaagac atgactgctg cagaactgaa agctgcaccg gaaaatgcgg tggcgaacgc
     3481 ttacgatatg gtcatcaatg gttacgaagt gggcggtggt tcagtacgta tccataatgg
     3541 tgatatgcag cagacggtgt ttggtattct gggtatcaac gaagaggaac agcgcgagaa
     3601 attcggcttc ctgctcgacg ctctgaaata cggtactccg ccgcacgcag gtctggcatt
     3661 cggtcttgac cgtctgacca tgctgctgac cggcaccgac aatatccgtg acgttatcgc
     3721 cttcccgaaa accacggcgg cagcgtgtct gatgactgaa gcaccgagct ttgctaaccc
     3781 gactgcactg gctgagctga gcattcaggt tgtgaagaag gctgagaata actgatatga
     3841 ctcaaataca cgaaatcatt cgcgttgcat caaggcggca actgagtgaa ctcccatgag
     3901 catagataac tatgtgaatg ggatgagcga aggcagtcaa cgaagaggca gcgtggagga
     3961 taaagtgtat aagcgtcccg tttcgatctt agtggtcatc tatgcacaag atacgaaacg
     4021 ggtgctgatg ttgcagcggc gtgacgatcc cgatttctgg cagtcggtaa ccggcagcgt
     4081 ggaagagggt gaaaccgcgc cgcaagctgc catgcgcgaa gtaaaggaag aggtcaccat
     4141 tgatgttgtc gctgaacaac tgaccttaat tgactgtcag cgcacggtag agtttgaaat
     4201 tttttcacat ttacgtcatc gctatgcgcc gggcgtgacg cgtaatacgg aatcatggtt
     4261 ctgtcttgcg cttccgcacg agcggcagat cgttttcact gaacatctgg cttacaagtg
     4321 gcttgatgcg cctgctgcgg cggcgctcac taagtcctgg agcaaccggc aggcgattga
     4381 acagtttgta attaacgctg cctgaacagg cagggctatc tggagatatt tttatggcag
     4441 gtcatagtaa atgggccaac accagacatc gtaaagctgc gcaggatgct aagcgcggta
     4501 aaatcttcac taaaatcatt cgtgagctgg taaccgcggc taagctgggc ggtggcgatc
     4561 cggacgctaa cccgcgtctg cgtgcggcgg tggataaagc actgtctaac aacatgaccc
     4621 gtgacacact gaaccgcgca attgcacgtg gtgtgggcgg tgatgatgat gcaaacatgg
     4681 aaaccatcat ctacgaaggt tacggtcctg gcggcacggc aatcatgatt gaatgtctgt
     4741 ctgacaaccg caaccgtacc gttgctgaag tgcgtcatgc attcagcaaa tgtggcggta
     4801 acctcggtac agatggttcc gtagcctatc tgttcagcaa aaaaggcgtg atctccttcg
     4861 agaaaggcga tgaagacacc atcatggaag cagctctgga agcaggtgct gaagacgttg
     4921 tgacctatga tgacggcgcg attgatgtct acactgcatg ggaagaaatg ggtaaagtgc
     4981 gcgacgctct ggaagcggca ggtctgaaag cagacagcgc ggaagtttcc atgatcccgt
     5041 ccaccaaagc tgatatggat gcagaaaccg caccgaaact gatgcgtctg atcgatatgc
     5101 tggaagattg cgacgacgtg caggaagttt accataacgg tgaaatctct gatgaggtcg
     5161 cagcgactct ctgatgaggc ctgctaaaca gcaaaacgga gacgcgtgat ggctattatt
     5221 ctcggcattg atccgggttc gcgcgtgacc ggctatggcg taattcgcca ggtaggcagg
     5281 caactgtcct acctgggtag cggatgcata cgcaccaaag tggatgattt accgtctcgt
     5341 ctgaagctca tctatgcggg cgtgacggaa atcatcaccc agttccagcc tgattatttc
     5401 gccattgaac aagtctttat ggcgaagaac gctgactcag ccctgaaact gggccaggcg
     5461 cgcggcgtgg cgattgtggc ggcggtgaat caggagttgc cagtatttga atacgcggca
     5521 cgtcaggtaa agcaaacggt ggtaggcatt ggtagcgccg agaaaagcca ggtgcagcat
     5581 atggtccgca ccttgctgaa actgcccgct aatccacaag cggatgccgc cgatgcactg
     5641 gcgattgcca tcacccactg ccacgttagt cagaatgcga tgcagatgag cgaatcgcgg
     5701 ctgaacctgg cgagagggcg actgcgttaa gttataccgc ctcggtcagt tcaggctgag
     5761 gcgtttcaac tccctccgcg tgaatcaaca ccaaacccgg atgatgccac aaactggctg
     5821 gcgtgacggt tttacgctcc cacggaatag aacctaagaa ccagaatttc cagaaagtga
     5881 gttttgcatt cggattgcta tttaacagtt ctccaaacgt ttctatttca cccaccggaa
     5941 tgcatagcgc ttctttataa atatcaaaaa caaattttga acagaactgg cgcgaagatt
     6001 cgtatttaaa accggtgtgg taaagtttgc gtagccggga gggaacctgt tcaacaattc
     6061 gttgtttttg ttgttctgtc agcccagcgt ctaatcgctt tatagcatag cgttgattag
     6121 acgaacgttt aataaaacga gatagcgtgg tgatggttga aaggggaacc cggctttctg
     6181 caaccaggaa gtcttcaccg ttatgaccga taatgatccc gacgtgatta ctccagcaat
     6241 ttgatgcagc tgatatttga ccaaataagg cagcacctat acatgtaaag acgatatatc
     6301 caatttcata ttcagcaggg taattaatat tc
//
LOCUS       DAZFQD010000203         6284 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.203, whole genome shotgun sequence.
ACCESSION   DAZFQD010000203 DAZFQD010000000
VERSION     DAZFQD010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6284
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.203"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            64..1614
                     /locus_tag="ACHKTW_004831"
     CDS             64..1614
                     /locus_tag="ACHKTW_004831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000019324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MchC protein"
                     /protein_id="HFO6465707.1"
                     /translation="MSHQCSLSELNENLVPFTARQIKASLIWCAEDVRNPDELQNACS
                     YIIDPGSTASAKVFHAERYGGSGIQRNGGGARCGFDGNYQVKGIGSNPLVGEGTDERH
                     SNGALGAVHAIYEALWGEVLAQILPYSAVRVRAVLLTDLYTEKAFERSGRKSRRALLV
                     REPVVRPAHFERAPYFQVKPEYSSLLIHDACRVRSVIHKLPGYLPVPPEEIDAEARTD
                     PRIYCIEGLCELARREAWQMAFCRTRFLRLTTSPSNIAMDGRLMDFNGLSCLFPGDSP
                     ADFGYKLRLAELAKEPMVLMQGLSDLCLYIGKYMFDPDFTLAARLKVEEIFQKTFHEA
                     CYYCYLELLGIPGEFITQKEIPDILKELVNSFVALLNQYCEKSHAQDIVNQDGSPLQK
                     LVVTLIHHRHNQKQALNSSIKNDVYFTVAQQCFSQAIHWLTQGSTRRQINASLLLKEI
                     EHHTMKRLQPREELRKENMCEKIAILLDNHGDDPLFLQEAISDMKNFMLKFSRDAFGY
                     LEPIRNTV"
     gene            1640..2092
                     /locus_tag="ACHKTW_004832"
     CDS             1640..2092
                     /locus_tag="ACHKTW_004832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009404 - toxin metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-activating lysine-acyltransferase"
                     /protein_id="HFO6465708.1"
                     /translation="MSYIRETIRGKDEWTVYEQIGFAVSCMLYNRNYSLYPVLTIQYW
                     TEYAIQHNQIKFLFDSRGFPVAYITWAYLEADTEARLLRDPEFRLHPSEWNEDGRIWI
                     LDFCCKPGFGRKVIDYLIQLQPWGEGEVRWLSRRKKIVTYIPERLHKT"
     gene            2244..3518
                     /locus_tag="ACHKTW_004833"
     CDS             2244..3518
                     /locus_tag="ACHKTW_004833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFO6465709.1"
                     /translation="MFRQDALENRKMKWQGRAILLPGIPLWLIMLGSIVFITAFLMFI
                     IVGTYSRRVNVSGEVTTWPRAVNIYSGVQGFVVRQFVHEGQLIKKGDPVYLIDISKST
                     RSGIVTDNHRRDIENQLVRVDNIISRLEESKKITLDTLEKQRLQYTDAFRRSSDIIQR
                     AEEGIKIMKNNMENYRNYQAKGLINKDQLTNQVALYYQQQNNLLSLSGQNEQNALQIT
                     TLESQIQTQAADFDNRIYQMELQRYELQKELVNTDVEGEIIIRALTDGKVDSLSVTVG
                     QMVNTGDSLLQVIPENIENYYLILWVPNDAVPYISAGDKVNIRYEAFPAEKFGQFSAT
                     VKTISRTPASTQEMLTYKGAPQNTPGDSVPWYKVIAMPEKQIIRYDEKYLPLENGMKA
                     ESTLFLEKRRIYQWMLSPFYDMKHSATGPLND"
     gene            3511..5613
                     /gene="mchF"
                     /locus_tag="ACHKTW_004834"
     CDS             3511..5613
                     /gene="mchF"
                     /locus_tag="ACHKTW_004834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin export peptidase/ABC transporter MchF"
                     /protein_id="HFO6465710.1"
                     /translation="MTNGNFRQIINQLDMRWRRRVPVIHQTETAECGLACLAMICGHF
                     GKNIDLISLRRKFNLSARGANLAGINGIAEQLGMVTRALSLELDELGALKMPCILHWD
                     FSHFVVLVSVKRNRYVLHDPARGRRYLGREEMSRYFTGIALEVWPGSEFLAETQQTRI
                     SLRSLINSIYGIKRTLAKIFCLSVVIEAINLVMPVGTQLVMDHAIPAGDRGLLTLISA
                     GLMFFILLRAAVSMLRAWSSLVMSTLINIQWQSGLFNHLLRLPLAFFERRKLGDIQSR
                     FGSLDTLRATFTTCVVGAIMDSIMVVGVFVMMLLYGGYLTWIVLGFTMVYVLIRLVTY
                     GYYRQISEETLVRGARASSYFMESLYGIATVKIQGMAGIRGTHWLNLKIDAINSGIKL
                     TRMDLLFGGINTFVAACDQVAILWLGASLVIDNQMTIGMFVAFGSFRGQFSDRVASLT
                     SFLLQLRIMSLHNERIADIALHEKEEKKPEIEIVADMSPVSLETTDLSYRYDSQSAQV
                     FSGLNLSVAPGESVAITGASGAGKTTLMKVLCGLFEPDSGKVLVNGTDIRQLGINNYH
                     RMIACVMQDDRLFSGSIRENICGFAEETDDEWMTECAKASHIHDVIMKMPMGYETLIG
                     ELGEGLSGGQKQRIFIARALYRKPGILFMDEATSSLDTESERFVNAAIKKMNITRVII
                     AHRETTLRTVDRIFRITN"
     gene            complement(5674..5997)
                     /locus_tag="ACHKTW_004835"
     CDS             complement(5674..5997)
                     /locus_tag="ACHKTW_004835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001390454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6465711.1"
                     /translation="MRNITTDELALIHGAGLDKDASVAIGTAAGGFLGKATKIPGADI
                     AGSAIGGYIGGVIADGSNRTITIPSNGINYNPGIGLGSFNPNYNGGLLNSGFNSSTSS
                     SSSGS"
BASE COUNT         1832 a         1180 c         1482 g         1790 t
ORIGIN      
        1 ggacacttac ttgctctttt atgaacactc ttttatgaaa attgaaacgg agaatactgc
       61 cagatgagtc atcagtgttc actttctgaa ctgaatgaaa acctggtgcc tttcactgcc
      121 aggcagatca aggcctcatt aatctggtgt gcagaggatg tcagaaatcc agacgaactg
      181 caaaatgcct gcagttatat tatcgatcct ggcagtacag cttctgccaa agtgttccat
      241 gcagagcgct atggtggcag tggtattcag cgtaatggag gtggtgcacg ttgtgggttt
      301 gatggtaact accaggttaa aggaatagga agtaatccgt tggttggcga aggtactgac
      361 gaacgtcatt ctaatggtgc actcggcgct gttcatgcaa tatatgaggc tttgtgggga
      421 gaagtactgg ctcaaatatt accttatagt gctgtgcggg ttcgggcggt tttacttaca
      481 gatctctata ctgaaaaggc atttgagcgc tccggtagga aatcacgaag agccctgttg
      541 gtacgtgagc ctgttgttcg tccggcgcat tttgaacggg caccttactt ccaagtaaaa
      601 ccggagtatt ccagtctgtt aattcacgat gcctgtcggg ttagatctgt gatccacaag
      661 ctgccaggat atctacctgt accaccggaa gaaattgatg ctgaagcacg aactgatccc
      721 cggatttatt gcattgaggg gttatgtgaa ctggcacgtc gtgaggcctg gcaaatggca
      781 ttttgtcgaa cacgtttcct gagattgaca acttctcctt ctaatattgc aatggatggc
      841 agattaatgg attttaacgg actcagttgt ctgtttccgg gagattcccc agctgatttt
      901 gggtataaac taagattagc tgaactggca aaagaaccga tggtacttat gcaagggctg
      961 tctgatctct gcttgtatat cggaaaatat atgtttgacc ctgacttcac tcttgcagcc
     1021 cgtttgaagg ttgaggagat atttcagaaa acttttcatg aagcatgtta ttactgttat
     1081 ctagaactgt tgggtattcc tggagaattt ataacacaaa aagagatacc tgatatattg
     1141 aaagaactgg ttaacagttt tgttgcatta ctcaatcaat actgcgagaa atcacatgcc
     1201 caagatattg tcaatcagga tggttcacca ttgcaaaagt tggttgtgac gctaatccat
     1261 cataggcata atcaaaagca ggcactgaat agtagcatca agaatgatgt ttatttcacc
     1321 gttgcacaac agtgtttttc ccaggccatc cactggctga cgcaaggcag taccagacgt
     1381 cagataaatg cctcattact cctgaaagaa attgaacatc ataccatgaa aaggctgcaa
     1441 cccagggaag agctgaggaa agaaaatatg tgcgaaaaaa ttgccatcct gctggataat
     1501 catggcgatg atcccctttt tttacaagaa gcaatttctg atatgaaaaa ttttatgctt
     1561 aagttttcca gagatgcatt tggatatctt gaaccgataa gaaacacagt gtaaaaaata
     1621 tataagggaa atagtaatca tgtcttatat aagggaaacc atcagaggaa aagatgaatg
     1681 gactgtttat gaacagatcg gttttgcggt cagttgtatg ctctacaatc gtaattacag
     1741 tctgtatccg gtgttaacca ttcaatactg gactgaatat gcgatacagc ataatcagat
     1801 taaattcctg tttgattcac gaggttttcc agtggcgtat attacctggg catatcttga
     1861 ggctgataca gaagcgcgcc tgctcaggga tccagaattc aggttgcatc cgtctgaatg
     1921 gaatgaagat ggaaggattt ggattctgga tttctgttgt aaaccaggct ttggtcgaaa
     1981 agttattgac tatctcatac agcttcagcc atggggagaa ggagaagtac gatggttaag
     2041 caggcgaaag aaaattgtga catacatccc tgagcggctg cataaaacgt agtacctctg
     2101 aagatacaga gataattgta aattacgggg taaatgcatc gctgatacta ttttgcagga
     2161 ctctgtattt tctgtctttg attatagcat ttcgcttaac cagtgaaggt attacaggaa
     2221 aagtgtgagt aagaggagct attttgtttc gtcaggatgc tttagaaaac agaaaaatga
     2281 agtggcaggg acgggcaata ttacttcccg gaataccact atggttaatc atgctgggaa
     2341 gcatagtgtt tattacggca tttctgatgt tcattattgt tggtacctat agccgccgtg
     2401 ttaatgtcag tggtgaggtc acaacctggc caagagctgt caatatatat tcaggtgtac
     2461 agggatttgt tgtcaggcag tttgttcatg aaggacagtt gataaaaaaa ggggatcctg
     2521 tttatctgat tgacatcagt aaaagtacac gtagtggtat tgtcactgat aatcatcgtc
     2581 gggatataga aaaccagctg gttcgtgtgg acaacattat ttcccgtctg gaagaaagta
     2641 aaaaaataac gctagatacc ctggaaaaac aacgcctgca atacacagat gcgtttcgtc
     2701 gctcatcaga tattatacag cgtgcagagg aagggataaa aataatgaaa aacaatatgg
     2761 agaattacag aaactatcag gcaaaagggc tgattaataa agatcagtta actaaccagg
     2821 tggcattata ttatcagcaa caaaacaatc ttctcagcct gagcggacag aacgaacaga
     2881 atgccctgca gataaccact ctggagagtc agattcagac tcaggctgca gattttgata
     2941 accgtatcta ccagatggaa ctgcaacggt acgagttaca gaaagaactg gttaacactg
     3001 atgtggaggg cgaaatcatt atccgggcgt tgactgacgg gaaagttgac tccctgagtg
     3061 tcactgtcgg gcaaatggtc aataccggag acagccttct gcaggttatt cctgagaaca
     3121 ttgaaaacta ttatcttatt ctctgggtcc cgaatgatgc tgttccttat atttcggctg
     3181 gtgacaaagt gaatattcgt tatgaagcct ttccggcaga aaaatttggg cagttctctg
     3241 ctacggttaa aactatatcc aggactcctg cgtcaacaca ggaaatgttg acctataagg
     3301 gagcaccaca gaatacgccg ggcgactctg ttccctggta taaagtcatt gcgatgcctg
     3361 aaaagcagat tatcagatat gacgaaaaat acctccctct ggaaaatgga atgaaagccg
     3421 aaagtacact atttctggaa aaaaggcgta tttaccagtg gatgctttct cctttctatg
     3481 acatgaaaca cagtgcaaca ggaccgctca atgactaacg ggaatttcag acaaattata
     3541 aatcagcttg atatgcgctg gcgacgtcgt gttccggtta ttcatcagac ggagaccgct
     3601 gaatgtggac tggcctgcct ggcaatgata tgcggtcatt ttggtaagaa tattgacctg
     3661 atatctcttc gccggaagtt taatctctcg gcccgtggag caaaccttgc aggaatcaat
     3721 ggaatagcgg agcagctggg gatggtcacc cgggctcttt cactggagct ggatgaactt
     3781 ggtgccctca aaatgccgtg tattctccac tgggatttca gtcacttcgt cgtgctggtc
     3841 agcgtaaagc gtaaccgtta tgtactgcat gatccggcca gaggcagaag atatctcggt
     3901 cgggaggaaa tgagccggta ttttacgggc attgcacttg aggtctggcc cggaagtgaa
     3961 ttcctggcgg aaacccagca gacccgcata agtctccgtt cactgattaa cagtatttac
     4021 ggtattaaaa gaacactggc gaaaattttc tgtctgtcag ttgtaattga agcaatcaat
     4081 ctggtaatgc cggtgggaac tcagctggtt atggatcatg cgattccggc gggggacaga
     4141 gggctgctga cgcttatttc tgctggcctg atgttcttta tattgctcag ggccgcggtg
     4201 agtatgctgc gtgcatggtc ctcactggtt atgagcacgc tcatcaatat acagtggcag
     4261 tcgggtctgt ttaaccatct tctcagactg ccgctggcgt tttttgaacg ccgtaaatta
     4321 ggtgatatcc agtcgcgttt tggctccctt gacactttga gggccacctt taccacctgt
     4381 gttgttgggg caatcatgga cagtattatg gttgtgggtg tttttgtgat gatgctgtta
     4441 tatggaggat atcttacctg gatagtgctc ggttttacca tggtttacgt tcttattcgt
     4501 ctggtgacat acggctatta ccggcaaata tcggaagaaa ctcttgtcag gggggcccgg
     4561 gccagctcct attttatgga aagcctgtat ggtattgcca cggtaaaaat ccagggtatg
     4621 gctgggattc ggggaacaca ctggcttaac ctgaaaatag atgcgatcaa ttcaggtatt
     4681 aagttaacca ggatggattt gctcttcggg gggataaata cttttgttgc cgcctgtgat
     4741 caggtggcga ttttatggct gggtgcaagc cttgtgatcg ataatcagat gacaataggg
     4801 atgtttgtgg catttggttc ttttcgtggg cagttttcgg atcgggttgc ttcgctgacc
     4861 agttttcttc ttcaactgag aataatgagt ctgcataatg agcgcattgc agatattgca
     4921 ctacatgaaa aggaagaaaa gaaaccggaa attgaaatcg ttgctgacat gagcccggtt
     4981 tcactggaaa ccactgattt aagctaccgg tatgacagcc agtcagcaca ggtattcagt
     5041 ggtctgaatt tgtctgtggc tccgggagaa agtgtggcta taactggtgc ctccggtgcc
     5101 ggaaaaacca cattaatgaa agtattatgt ggactgtttg aaccagatag tggaaaagta
     5161 ctggttaatg gcacggatat acgtcaactt ggaataaata attatcaccg tatgatagcc
     5221 tgtgttatgc aggacgaccg actattttca ggatcaattc gtgaaaatat ctgtgggttt
     5281 gcagaagaaa cagacgacga atggatgaca gaatgtgcca aagcaagtca tattcatgat
     5341 gtgataatga aaatgccaat ggggtatgaa acgttaatag gtgaactggg ggaaggtctt
     5401 tccggcggtc aaaaacagcg tatattcatt gcccgagctt tataccggaa acctggaata
     5461 ttatttatgg atgaggctac aagttctctt gatacagaaa gtgaacgttt cgtgaatgct
     5521 gctataaaaa agatgaatat cacccgggtg attattgcac atagagagac aactttaaga
     5581 acagttgatc ggatttttag aattacaaat taggataata tatcactaaa agcagcattg
     5641 ctgcataata agctatgctg ctttagaaat taatcaactt ccactactag aagaggatgt
     5701 tgatgagtta aatcctgagt ttaataaacc tccattgtag tttggattaa aacttccaag
     5761 tcctataccc ggattatagt taataccgtt tgatggtatt gttattgtcc tatttgaacc
     5821 atcagcaata actcctccaa tatatcctcc aattgcactt cctgctatat ccgcaccagg
     5881 gatttttgtt gctttaccta aaaaaccacc agccgcagta cctattgcaa cggatgcatc
     5941 tttatctaat cctgcaccat gtattaaagc aagttcatcg gttgttatat ttctcatgtt
     6001 atttcctctt tttattaaag aaagataaaa taaataaaaa cagtacactc gcaagtaacg
     6061 aaagaattat tttctttatg aaaagataaa gggttaatgt gtctaagtct gaatttataa
     6121 gttgtattat aaagaacaac gagaaataaa aaaatagttt cattattaaa ccatccctat
     6181 acatgagtct ttgagtgctt atgaaaaact tagaaataaa gactatagaa accaaggtag
     6241 caaaagtttt tatttatccg aatattttta cttcttgctg tttg
//
LOCUS       DAZFQD010000204         6270 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.204, whole genome shotgun sequence.
ACCESSION   DAZFQD010000204 DAZFQD010000000
VERSION     DAZFQD010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6270
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.204"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(10..627)
                     /locus_tag="ACHKTW_004836"
     CDS             complement(10..627)
                     /locus_tag="ACHKTW_004836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6465712.1"
                     /translation="MYGLSIMKPDGSVWISPGFTPQCLINKGTIPATEKSFFKTSIPS
                     GKSCFFFIRTEKKADVMYTHEQIDGYHALRLHVIVRGTNPGVTTVYAFANMVTPPSEY
                     GIAMYNPDGEMIYHGEMMLLDAKLIPVDIKFEKDLGYPCAIMPALVGYYNWKRTPYDR
                     PIYTTSTGATGNKIYSCEHYSGGATWDIRKPYIDKVLVINTSVYD"
     gene            complement(633..911)
                     /locus_tag="ACHKTW_004837"
     CDS             complement(633..911)
                     /locus_tag="ACHKTW_004837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6465713.1"
                     /translation="MEYGFAIYNRNNVNVTGVLTPVFFLDRFTAESGSKTYTNKPDGK
                     SLQAVCCLFPWNNVFADRKVPKITINGNTVTWSNLEQGMGSYIYTFWG"
     gene            complement(926..2194)
                     /locus_tag="ACHKTW_004838"
     CDS             complement(926..2194)
                     /locus_tag="ACHKTW_004838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HFO6465714.1"
                     /translation="MTRKPWRAGKDLSTVVENMEIGTGQRGDGRHAFVTREELVGLKL
                     ARRRTSGGASYALNPGIEIDSTLMTVDFPTKPLNFKATGGFGSVLLEWDMPNYRGHSL
                     TEIWRGTEDDLADAVLVATTPGQVYGDPVDPGWSGFYWIRFVNAAGVKGPWNAEKGTQ
                     AQTQIGVKAIIDQIRDEAAKSPVVSELRKEIKNAQGQAVKDAAIKTTEVVGTLREETT
                     RTIGGIETRISTLDSSTSESLNEVDKRITKLDKEGGEAFLAMWSKKAGVDGITAGIGI
                     VAGKDSEGRPVSQVAISASQLFVFDPNNPDNTAYPFAVSGGKVVIPKAMIYDAVIETL
                     VSRKVVADEVKAGVSITSPVIRSAVIQNGNFQVDSQGNLNIGGLFSVTSQGQLTIRYS
                     NQNVGLVIRNDKIEVYDQNGRLAVRIGRLR"
     gene            complement(2191..3816)
                     /locus_tag="ACHKTW_004839"
     CDS             complement(2191..3816)
                     /locus_tag="ACHKTW_004839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6465715.1"
                     /translation="MPYIDITTMRGMMPGVIASMLPDHSAVLAENCHFRYGVITPEHQ
                     MSEAEKTFAIKPKTIFHYRDDFWFAWTDVVDVIRSPVAQDSHGRIYYTDGRFPKVTDA
                     TIATKGDGNHPTSSYRLGIPAPTTAPVCTVQQGGDVSDDNPNDDETRFYTETFVSDYG
                     EEGPPGPASLEVTLRTPGTAVQLTLSPVPLQNASIKRRRIYRSASGGGEADFLLVAEL
                     DASVLSYTDKIPGKNLGPSLATWDYLPPPENMTGLCLMANGIAAGFAGNEVMFSEAYL
                     PYAWPEVNRHTTAEDIVAICPLGTSLVVATKGEPYLFSGVSPSTISGSRIPSMQACLS
                     RRSMVAMEGFVLYAGTNGLVSVDVNGNTALATEKIISPEQWQSQFNPASIVAYSWRGE
                     YIACYTKPDGKQDVFVFSPVNMDIRYLSTPFDCAWVDLAKDMMRVVTGDKMSVLAGSS
                     LPSTIRWHSKIFSLPERTSFSCIRVKSPAPERVGITIMADDVPVIHFAPGTFKGSVVR
                     LPAATGQNWQVMVSGFGQVERITLSTSMSEMPV"
     gene            complement(4050..4562)
                     /locus_tag="ACHKTW_004840"
     CDS             complement(4050..4562)
                     /locus_tag="ACHKTW_004840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6465716.1"
                     /translation="MGITSGWVGSSAKSETGEQWMGAAGTKLGLDKPFMMSQMVGRAM
                     GCKIETAYYKWNSSDQVENWGAVGADWPLEEKSKGTITNAENCGSGRLVGAVVTLSHF
                     LTNSTPTAAVYLSGGKAGNITVNVGGATQTMIYQGVVSGFQYYWSGSVSSAFVEAMKK
                     TGVTQDLKIS"
     gene            complement(4649..>6270)
                     /locus_tag="ACHKTW_004841"
     CDS             complement(4649..>6270)
                     /locus_tag="ACHKTW_004841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000117976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019062 - virion attachment to host cell
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HFO6465717.1"
                     /translation="GDMGPKGEKGDPGGPAGPQGPKGERGEAGPQGPMGARGERGETG
                     PRGEPGPAGPRGERGETGPQGPRGEPGPAGSAANVADATTAQKGIVQLSSATDSDDET
                     KAATPKAVKAAMDKADGCLEKAKNGDDIPDKVKFLNTVGAARVYGRDIHTGAGEWTTS
                     EFVAWLKEKGAFDQPYWMMKASLHAEFNKVITDVGPGKLNLGGCAIEVMGTYNAAIVR
                     VTIGEYGGDGFLNGTVCTCTVYGDTQRFHWRVDYSTKNKPDTVSQRDASTTQKGVVQL
                     SSDTDSNDETKAATPKAVKAAMDVANEAKTKAEEAAAGGGVPGPKGEKGDTGPAGPAG
                     PQGPKGDTGAAGPAGAQGPKGDKGDPGVAGPAGPAGAPGPKGDKGDPGVAGPAGPEGP
                     QGPKGDTGAPGQGTELLTTANTWTQAQTFNGGINGNLTVNGNGSFNDVQIRSDKRNKR
                     NLVKLDNALDRLEALTGYLYEIQYSADGWQTSVGLIAQDAQKALPELVTEDADVISGE
                     KRLRLNYNGIIALLVEGFKTLRHEIKELREK"
BASE COUNT         1480 a         1817 c         1290 g         1683 t
ORIGIN      
        1 tcgcttcaac taatcatata ctgatgtatt aataaccagg accttatcta tatacggctt
       61 tcgaatatcc catgttgcac cgccggaata atgctcacag gaatatattt tatttcctgt
      121 agcaccagtg gatgtggtat aaatcggtcg atcataagga gttcttttcc agttataata
      181 cccgaccagt gcaggcatga ttgcgcatgg atatccaagg tccttttcaa atttgatatc
      241 aacaggtatt aacttcgcgt caagcagcat catttcgcca tgataaatca tctcaccgtc
      301 cgggttatac atggcgatac catactcaga aggtggagta accatattcg cgaaagcata
      361 aaccgtcgta acaccagggt tcgttcccct gacaattaca tgaagcctta gtgcatgata
      421 tccatcaatc tgttcatgcg tgtacatgac atcggccttc ttctctgttc tgataaagaa
      481 aaaacaactt ttgcctgacg ggattgatgt tttaaaaaaa gacttttcag tcgccggtat
      541 ggtgcctttg ttgatcagac actgcggcgt aaaacctgga cttatccata cgctgccatc
      601 cggcttcata atgctcaaac catacatgac acttatcccc agaatgtata aatataagat
      661 cccataccct gctcaagatt cgaccacgtc accgtattgc cattaatggt tatcttcggt
      721 actttccgat ccgcaaatac gttattccag ggaaataaac aacatacagc ctgcaatgat
      781 ttcccgtcgg gtttattcgt gtacgtcttt gagccagact ccgctgtaaa tctgtccagg
      841 aaaaataccg gagtcagcac gcccgtaaca ttaacgttat ttctgttata aatggcaaaa
      901 ccgtattcca atactcacct cctgatcagc gtaatctgcc tatgcgaaca gccagtcgtc
      961 cattctgatc ataaacctca attttatcat tgcggatcac cagtcctaca ttctgattag
     1021 agtaacgaat tgtcagttgc ccttgtgacg taacactgaa aaggcctcca atattcaggt
     1081 taccctgaga atcaacctga aagtttccgt tctgaataac ggcactccgg ataactggcg
     1141 aagtgatact taccccggct tttacctcat ccgccacaac cttccgcgac accagtgttt
     1201 caatcaccgc gtcataaatc atcgctttcg ggatcacaac cttgccacct gataccgcaa
     1261 acggataggc ggtgttatcc gggttgttcg ggtcaaagac aaacaactgc gacgcagaaa
     1321 ttgcaacctg acttacaggc ctaccttcac tgtcttttcc ggcgacaatc ccgatccccg
     1381 cagtgatacc atcaactccc gctttttttg accacattgc cagaaacgcc tcaccgcctt
     1441 ctttatccag tttagtgatg cgcttgtcga cctcattaag cgattcactg gttgatgaat
     1501 ccagtgtgct aatgcgggtt tcaataccac caatcgttct tgtcgtttct tccctgagag
     1561 tccccacaac ttcggttgtc ttaattgcag catcctttac agcctgcccc tgcgcgtttt
     1621 ttatttcttt acgcagctcg gacacaaccg gcgactttgc agcctcatcg cggatctggt
     1681 caatgatggc cttcacgccg atctgtgttt gtgcctgagt gcctttttca gcattccatg
     1741 gacctttcac tcctgccgcg ttaacaaaac gtatccagta aaatcccgac cagccagggt
     1801 caaccggatc gccgtaaacc tgccccggcg tcgtggcaac cagcactgca tcagcaaggt
     1861 catcctccgt accccgccag atttcagtca gtgaatgtcc gcgataatta ggcatatccc
     1921 attcaagaag aaccgagcca aatcctcctg tcgccttaaa attcagcggt tttgtgggaa
     1981 aatcaacagt cattaaagta ctgtcaatct caatacccgg attcagtgca tatgaggcac
     2041 cacccgatgt tcgacgccgg gcgagtttaa gaccaaccag ttcctcacgg gtcacaaatg
     2101 cgtggcgtcc gtcaccacgc tgcccggtgc caatttccat gttctccaca actgtggata
     2161 aatccttccc cgcacgccac ggttttctgg tcataccggc atctccgaca tcgatgtact
     2221 cagggttatt cgttccacct gcccgaatcc ggataccatc acctgccagt tttgcccggt
     2281 tgctgccgga agtctcacca cacttccctt aaacgtaccc ggcgcaaaat gaatcacagg
     2341 aacatcatca gccataatgg tgatccccac ccgctcaggc gccggagatt tcaccctgat
     2401 acaggaaaaa gaggttcttt caggtaatga aaaaattttt gaatgccacc ttatcgtgga
     2461 gggcagagag ctcccggcaa gcactgacat tttgtctcct gtcaccacgc gcatcatatc
     2521 tttcgcgaga tcaacccatg cgcagtcaaa cggtgtactg agataacgga tatccatgtt
     2581 caccggactg aatacaaaca catcctgctt accatccggt ttcgtgtaac aggcaatgta
     2641 ctcaccacgc caggaataag ccacaatgga cgccgggtta aactgactct gccactgttc
     2701 aggtgaaata atcttttccg ttgccagtgc tgtattaccg tttacatcaa cagataccag
     2761 accgtttgtc ccagcataga gtacgaatcc ctccatcgcc accatacttc ttcggctcag
     2821 gcatgcctgc atggaaggaa ttctggagcc agaaattgtg gaaggcgata ccccactgaa
     2881 cagatagggc tccccctttg tcgccaccac cagtgacgtt cccagcggac agatagccac
     2941 aatatcttct gccgtcgtgt gacgattcac ttccggccat gcatacggca gatacgcttc
     3001 cgaaaacatc acttcattac cggcaaaccc ggcggcaata ccattagcca tcaggcaaag
     3061 gcctgtcata ttctctggcg gcggcaggta atcccatgtc gccagagaag gtccaaggtt
     3121 tttccccggt attttgtccg tgtaactgag cacggatgca tccagttcag ccacaagtaa
     3181 aaaatccgct tctcctccac ctgatgcaga gcgataaatc cggcggcgtt taatactggc
     3241 attctgcaat ggcaccggag acagcgtcag ctgtaccgca gtccccggag tacggagtgt
     3301 tacctccaga gacgccggac ctggcggacc ttcttcacca taatctgaga caaaggtttc
     3361 cgtataaaac cgggtttcgt catcattcgg gttatcgtca gaaacatcac cgccctgctg
     3421 aacagtacag acaggtgctg tcgtcggcgc ggggatcccc agacgatacg atgatgtcgg
     3481 gtgattcccg tccccttttg tggcaatggt cgcatccgtc actttaggaa aacgcccgtc
     3541 agtgtagtaa atacgcccgt gggagtcctg agcgaccgga ctgcggatca catccaccac
     3601 atccgtccat gcaaaccaga aatcgtcacg gtaatgaaaa atggttttcg gcttaatcgc
     3661 gaatgttttc tcagcctctg acatctggtg ttcaggcgtg atcactccat agcgaaaatg
     3721 acagttttct gccagtacag cagaatgatc tggcagcata gatgcaataa cgcctggcat
     3781 catcccgcgc atagttgtta tatcgatata aggcatggtt gtctggtatc cgaaaatttg
     3841 aatggcaggt gagtcactga attgaagttt taaaggcggc atacaattgc gcctgtgcag
     3901 aatacagatt tccagaaatc ttgaaaaatt acatcattat tatcagcatc cgtccggaaa
     3961 aatttctctg gcaacttaaa acaatatcct atatatccgg cgtctccggt atgtagaacg
     4021 tgaagttttt aaaattattc atttaccatt taactaattt ttagatcctg ggttactccc
     4081 gtctttttca ttgcctccac gaaagcggaa ctaacagaac ctgaccagta atactggaac
     4141 ccactaacaa cgccctgata aatcatggtt tgtgtagcac cacctacgtt tacggtgatg
     4201 ttacctgctt taccaccgga taaataaaca gcagctgtcg gtgtagagtt cgtcaaaaag
     4261 tgagaaagtg taacgacagc ccccaccagt ctcccagaac cacagttttc agcgtttgta
     4321 attgtccctt tgcttttttc ttctaatggc caatcagctc caaccgcccc ccagttttca
     4381 acttgatccg aagagttcca tttatagtaa gcggtttcta ttttacaacc catagctcgc
     4441 cccaccattt gactcatcat aaaaggttta tccaatccta gtttagtgcc agcagccccc
     4501 atccattgct cacctgtttc gctcttagcc gaagaaccta cccatcccga tgttatcccc
     4561 atttcatata tccttctgaa attcgtgagg tgagtatcat ccccacaatt taaattcagt
     4621 agaaaccaga aactacaaca gctgtcgttt acttctcccg gagttcttta atctcatgac
     4681 gaagtgtttt aaagccttcg actaacaatg caattatgcc gttgtagtta agacgcagac
     4741 gtttttcacc agatataacg tctgcgtctt cagttaccag ttcaggcaag gctttttgtg
     4801 catcctgagc aattaaacca accgacgttt gccaaccgtc ggcagagtac tgtatctcgt
     4861 aaagataacc agtaagtgcc tccagacgat ctaacgcatt atccagtttt accagatttc
     4921 gcttgttgcg tttatccgag cggatctgaa catcgttaaa tgatccgttt ccgtttaccg
     4981 tcaaatttcc attaatacca ccattaaaag tttgtgcctg agtccatgta ttggcagtag
     5041 taagcagttc tgttccttgc ccgggggctc cagtgtctcc cttcggtccc tgcggccctt
     5101 ccggacctgc tggacctgct actcccggat cacctttatc gcctttcggc cccggcgcac
     5161 ctgccggacc tgctggtcca gccacccccg gatcgccttt gtcacctttt ggtccctgtg
     5221 cgcctgccgg gcctgcagct cccgtatccc ctttaggtcc ctgcggtcct gcaggacctg
     5281 ctggccccgt gtcccctttc tctcctttcg gaccgggaac accacctcct gctgcagcct
     5341 cttctgcctt tgttttcgct tcatttgcca catccattgc cgccttcacg gccttcggcg
     5401 tggctgcctt tgtttcgtca ttactgtcag tatcactgct taactgcacc acacctttct
     5461 gcgtcgtgct ggcatcccgc tggctgaccg tatccggttt gttttttgtg ctgtaatcca
     5521 cccgccagtg aaaacgttgt gtgtctccgt aaactgtaca ggtacagacc gtgccgttca
     5581 gaaaaccatc gccaccgtat tcgccgatgg tgacccggac tatggctgcg ttatacgtcc
     5641 ccatcacctc aatggcgcag cctcccagat tgagttttcc cggtccgaca tccgtgatga
     5701 ccttattaaa ttccgcatgc agtgatgcct tcatcatcca gtaaggctgg tcaaatgccc
     5761 ccttttcttt cagccaggcc acaaactcac tcgtggtcca ttcaccggcc cccgtatgaa
     5821 tgtcccgccc gtatactctg gctgctccca cggtgttcag aaacttcacc ttatccggga
     5881 tatcgtcacc gtttttcgct ttctccaggc atccgtccgc tttgtccatt gccgctttca
     5941 ccgctttcgg ggtggctgcc ttcgtttcat catcactgtc cgttgcgctg cttaactgca
     6001 caattccctt ctgtgccgtc gtcgcatcag ccacatttgc agcgctgcct gccggacctg
     6061 gctctccacg aggtccctga ggtccggtct ctcctcgttc gcctctcgga cctgcaggac
     6121 caggttcacc tcgggggcca gtctccccac gctcacctcg tgctcccatc ggaccctgtg
     6181 gtccggcttc tcctcgttca cctttaggac cttgcgggcc tgcaggacct cccggatcac
     6241 ctttctcgcc ttttggcccc atatccccct
//
LOCUS       DAZFQD010000205         6073 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.205, whole genome shotgun sequence.
ACCESSION   DAZFQD010000205 DAZFQD010000000
VERSION     DAZFQD010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6073
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.205"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            27..86
                     /locus_tag="ACHKTW_004842"
     CDS             27..86
                     /locus_tag="ACHKTW_004842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6465718.1"
                     /translation="MRNAVRKAGITSRRRLLLF"
     gene            complement(75..227)
                     /gene="hokA"
                     /locus_tag="ACHKTW_004843"
     CDS             complement(75..227)
                     /gene="hokA"
                     /locus_tag="ACHKTW_004843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012540334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HFO6465719.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQGSYELAAFL
                     ACNLKE"
     gene            complement(553..2622)
                     /gene="glyS"
                     /locus_tag="ACHKTW_004844"
     CDS             complement(553..2622)
                     /gene="glyS"
                     /locus_tag="ACHKTW_004844"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFO6465720.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLEAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRLNRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            complement(2632..3543)
                     /gene="glyQ"
                     /locus_tag="ACHKTW_004845"
     CDS             complement(2632..3543)
                     /gene="glyQ"
                     /locus_tag="ACHKTW_004845"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFO6465721.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            complement(3638..3937)
                     /gene="ysaB"
                     /locus_tag="ACHKTW_004846"
     CDS             complement(3638..3937)
                     /gene="ysaB"
                     /locus_tag="ACHKTW_004846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HFO6465722.1"
                     /translation="MMMNAFFPAMALMVLVGCSTPSPVQKAQRVKVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            4112..5107
                     /gene="wecH"
                     /locus_tag="ACHKTW_004847"
     CDS             4112..5107
                     /gene="wecH"
                     /locus_tag="ACHKTW_004847"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HFO6465723.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQSRHFLRIGLCLIFYSAIALLYIALFTSINM
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMVVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            complement(5149..5586)
                     /gene="yiaA"
                     /locus_tag="ACHKTW_004848"
     CDS             complement(5149..5586)
                     /gene="yiaA"
                     /locus_tag="ACHKTW_004848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaA"
                     /protein_id="HFO6465724.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTSIYYMTCLTVFIISVALLMVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDTGINPPKETQVTQEEYSE"
     gene            complement(5632..5973)
                     /gene="yiaB"
                     /locus_tag="ACHKTW_004849"
     CDS             complement(5632..5973)
                     /gene="yiaB"
                     /locus_tag="ACHKTW_004849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaB"
                     /protein_id="HFO6465725.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
BASE COUNT         1520 a         1532 c         1458 g         1563 t
ORIGIN      
        1 gaaaaataaa tttgggtgca atgagaatgc gcaacgccgt aagaaaggcg ggaataactt
       61 cccgccgaag actcttactc ttttaaattg caggctaaaa atgccgccag ctcataactc
      121 ccctgtttaa tatgcaattc acacagtgaa tctcttatca tccaggtgaa aaataaaagc
      181 gtgaaacaaa tcactattaa agaaagtaat ctatatttct gcggcattcc cagccccctg
      241 tgttgatttc caacgagtaa gaggctaacc ttatggtgtt caggcatagg gtagcctcac
      301 tttgatttat agtcaggtgg ggcttttctc tgtctgcctt tcggtgaata cctgagacaa
      361 acagtctcaa gcacccgtgg ctattctagc ttaataagtt tgtttcttct ccttgatata
      421 atcctaaaaa aacctcataa aattaatata tgagataatc tttattcagc agaagattat
      481 taaaggttgc tgtattattt agcgataata aaaaaagcct gccagatggc aggcttttta
      541 ataacggcgt tattactgca acagcgaaat atccgcaacg cgcaggaaca gttcgcgcag
      601 tttctccagc atggtcagac ggttgagacg caattctttg tcatcaacca tgaccatcac
      661 tttatcgaag aaagcatcaa ccggctcacg cagctctgcc agttcgacca gcgcatcctg
      721 gtaacgacct tccgcaaagt acggctccag cttgtcacgt agcaccacaa cctgcatcgc
      781 cagtttaatt tcttccggct ctttcagggt agaggcattc acgcggtcgc tcagcacttc
      841 gtcagatttc gccaaaatgt tggagacacg cttgttcgcc gccgccagtg cagcagctgc
      901 ttcaagggta cggaagtgcg ataccgcttt catgcgggca tcgaaatcag ccggacgagt
      961 cggacgacgc gccagtaccg cctggatggt gtcaacggtg taaccttcgt cctgatacca
     1021 ggcgcggaag cgaccgagca taaagtcgat aacatcatca accacgttgg cgttagtcag
     1081 cttatcgcca tacagacgca ctgcttcttc ggtcagcgtt tgcagatcaa ggttgaggtt
     1141 cttctcaacg ataattcgca gcacgccaag tgcggcacga cgcagcgcaa acgggtcttt
     1201 gtcgcctttc ggatgctggc cgataccgaa gatacccgcc agagtatcca tcttgtcagc
     1261 aatcgccagc gcacaggcta ccgggttaga cggcaggtca tcaccggcaa agcgcggctg
     1321 atactgctca ttcagcgcca ccgcgacatc ttccgcttcg ccatcgtgac gcgcatagtg
     1381 catccccata acgccctggg tgtcggtgaa ctcgaagacc atgttggtca tcaggtcaca
     1441 cttagacagc aggcccgcac gggttgcgtg gttaacgtca gcgccaatct gttcagcaat
     1501 ccagccagcc agcgcctgga tgcggtcagt tttgtcgcgc agcgtaccca gctgttgctg
     1561 gaacagcacg gtttgcaggc gcggcaggtt atcttccaga cgttttttac ggtcggtatt
     1621 gaagaagaac tcggcatccg ccagacgtgg acgaacgact ttctcgttac cagagataat
     1681 ttgctgcgga tctttcgatt cgatgttggc aacgaagatg aagttcggca gcagtttgcc
     1741 atcgttcgca tacaccggga agtatttctg gtcacctttc atggtgtaaa ccagggcttc
     1801 agccggcacc gcgaggaatt tctcttcgaa ttttgcggtc agaacgaccg gccactccac
     1861 cagcgaagcc acttcttcca gcaggctttc gcttaagtca gcgttaccgc caatcttacg
     1921 cgctgcttct tcggcatcgg ctttaatctt cgctttacgt tcttcgtaat cggcgatgac
     1981 tttcccgcgc tcacgcagaa tttccggata ctgatcggcg ttgtcgatgg tgaattccgg
     2041 ttcgcccata aagcggtggc cgcgaatcac gcgatcggac tgaatgccca gaatggttgc
     2101 cggaatgact ttgtcgccca gcagcagggt cacggtgtgc accggacgca cgaagtgcac
     2161 gtcgcttgcg ccccaacgca tcagtttcgg gatcggcagt ttcgccagag aagtcgcaac
     2221 catattcggc agcagtgctt cggtgctttc gcccttcaca tgggcgcgat acagcagcca
     2281 ttcgccttta tcggtagtca gacgctcagc ctggtcaacg gtaataccgc aaccacgcgc
     2341 ccaaccttct gccgctttgc tcggtttgcc ttcagcgtcg aacgcctggg caatcgccgg
     2401 gccgcgtttt tcgatttcac gatccggttg cgcttccgcc aggttagcta ctttcagcgc
     2461 cagacgacgc ggagcagcaa accattgaac ggtgccgtgt gcgaggccag cgttatccag
     2521 ctccgcagta aaattcgcag caaaggactc agccaggctg cgcagtgctt ttggtggcag
     2581 ctcttcagtg ccgatttcca ccagaaaagt tttctcagac atagccgcct cttacttatc
     2641 tttgttgcac atcgggaagc cgagggcttc acgggaagcg tagtatgctt ctgccactgc
     2701 tttggtcagg gtgcgaatgc gcagaatgta gcgctgacgc tcggtgacgg agatggcttt
     2761 acgcgcatcc agcaggttga agctgtgggc ggctttcaga atacgctcgt aggctggcag
     2821 cggcagcgga ttttccagcg ccagcagctg ctgcgcttct ttctcgtact gctcaaagca
     2881 ggtgaacagg aagtccacat ccgcgtattc gaagttgtaa gtggactgct ccacttcgtt
     2941 ctgatggaac acgtcgccgt aggtggtttt acccagcggg ccgtcgctcc agactaggtc
     3001 gtaaacgctg tctacgccct gaatgtacat ggccagacgt tccagaccgt aggtgatctc
     3061 gccggtaacc ggtttacact ccagaccacc aacctgctgg aagtaagtga actgcgtcac
     3121 ttccatgccg tttagccaca cttcccagcc cagtccccag gcacccagag tcgggttttc
     3181 ccagttatct tccacgaaac ggatgtcgtg aatagtcggg tccatgccca gctctttcag
     3241 agaaccgagg tacagctcct gaatattgtc cggcgatggc ttaatgacca cctggaactg
     3301 atagtagtgc tgtaaacggt tggggttttc gccgtagcga ccatcggtcg ggcgacgaga
     3361 aggctgaaca taagcagccg ccatcggttc tggccccagc gcgcgcagac aggtcattgg
     3421 gtgagaggtt cccgcgccga cttccatgtc caatggttga acaatggtgc agccctggcg
     3481 agcccagtaa tcctgtaagg tcaggatcaa gccctggaag gtcctggtat caaacttttg
     3541 catattattt cgtgctggat acgtgtggat ttaaaggaag ggatcagtat acccgctgga
     3601 tggaagatat acagtacgaa acgggaaaaa gcagggctta acgcatggaa agatgcaaaa
     3661 aatggccatc tgcgtcaaaa gaacagacaa actgctcttt acggaaggta taaccgcgca
     3721 tttcatagct tccctggaac tgttcgaagg cggtgacgtc gattttttgc tcgccggtgt
     3781 tataacgttt tgccgcctga tccttgcata acgcttccat attcaacgaa cgcagaggat
     3841 caacctttac ccgttgtgct ttctgcacgg gtgacggtgt agaacaaccc actagcacca
     3901 taagcgccat tgccggaaag aaagcgttca tcatcattaa attaccgtct ggtttgcagg
     3961 tgagtcttat tatttatatc ggtatagtgg ctgatatctt acgaatttcc ttaagcctct
     4021 ataagctgta gtgataaaac tcagattatt ccagtgcgcc aactgaatga tttatcggtc
     4081 agactttttt taaccagata cagagacaca gatgcagccc aaaatttact ggattgataa
     4141 cctgcgaggg atagcgtgtt taatggtggt gatgattcac accactacct ggtatgtgac
     4201 caatgctcat agtgttagcc ccgtcacctg ggatatcgcc aatgttctga attctgcctc
     4261 tcgtgtcagc gtgccgctat ttttcatgat ttccggctat ctcttttttg gcgaacgcag
     4321 cgcccagtcg cgccatttct tgcgtatcgg cttatgtctg attttttata gcgcaatcgc
     4381 actgctctac attgcgctgt ttacctccat caatatggag ttagcgctga aaaacctgct
     4441 gcaaaagcca gtgttttacc acttgtggtt tttcttcgcg attgcggtga tttatctggt
     4501 ttcaccgctg attcaggtga agaacgtcgg cggaaaaatg ttgctggtgc taatggtggt
     4561 gattggcatt atcgctaacc caaacacagt gccgcagaaa attgacggtt ttgaatggct
     4621 gccaattaac ttatatatca atggcgatac tttttactac attctgtatg gcatgttggg
     4681 ccgcgctata gggatgatgg acacacagca taaagcactg tcgtgggtga gcgccgcgct
     4741 gtttgcgacg ggggttttta ttatctctcg cgggacatta tatgaattgc agtggcgcgg
     4801 aaattttgcc gatacctggt atctttactg tgggccgatg gtttttatct gcgcaatcgc
     4861 gctattgact ctggttaaaa acacgctgga tacgcgtacc attcgcggac ttggcttaat
     4921 ctcccgccat tcattgggta tatacggatt ccacgccttg attatccatg cgctgcgcac
     4981 ccggggaatt gagcttaaaa attggccaat actggatatt atttggatct tttgcgcgac
     5041 gttggcagcg agtttgttac tttctatgct ggtacaacga atcgacagaa acagattagt
     5101 gagttaagta aaagcccgtt cacattggac tgaccgggct tacgtgaatt attcgctgta
     5161 ttcttcctgg gtaacctgtg tttcttttgg cgggtttatt ccggtatcac gaatattctt
     5221 ctgcaccgct actgcaccaa acaagcttaa gaagaaagcc agtccataaa aacctttttc
     5281 actgagtaat aatgtcgcat tccacagacc taccatcagt aatgcaacag agataataaa
     5341 gacagtcagg caggtcatat aataaatgga agtggtcggt atgccttcat acttgtcccg
     5401 aacggtcttt tgataagacg cagcagaaaa cagtcctaac accagtacgg caaaataata
     5461 ccctttttca tttaactgca tctctgcatt ccatagccct aacagatagg taacgatacc
     5521 accaacgaga gctatccatg acacaatact aaaggccggt gaataggttg atattttgtt
     5581 gtccatcaca gtattccttt cattcctgaa tgtgtaagag ctttatgttg ctcataccga
     5641 gcgaaacaaa cgcatttgac caaacaagca gacaaaaaag gcgatggggt agatcaccat
     5701 ttcataagca ttgataggtg catttaaaac accgataaac aagagaccga tagtgattaa
     5761 cgcaactaac tggcagatat gggtaaaaaa attatccagt tgcccgagtt tttctgcgcg
     5821 aagatatgca tagttaccaa aagttgctgt cattaacacg ccaagaaaat agccttttcc
     5881 acttagcaat gggcatgaga tccatagccc aaccagataa accagcgccc cgacaacaaa
     5941 taatagtttt gccacagttt ttgaggtttt cataacgatc tccatatcta ccagcgatac
     6001 attacgagta accaacgaaa gacaaaactg aaaaatgcca ttaacaaatg attttcagaa
     6061 taaattcata cta
//
LOCUS       DAZFQD010000206         6045 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.206, whole genome shotgun sequence.
ACCESSION   DAZFQD010000206 DAZFQD010000000
VERSION     DAZFQD010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6045
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.206"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            89..2179
                     /gene="iha"
                     /locus_tag="ACHKTW_004850"
     CDS             89..2179
                     /gene="iha"
                     /locus_tag="ACHKTW_004850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional siderophore receptor/adhesin Iha"
                     /protein_id="HFO6465726.1"
                     /translation="MRITTLASVVIPCLGFSASSIAAAEDVMIVSASGYEKKLTNAAA
                     SVSVISQEELQSSQYHDLAEALRSVEGVDVESGTGKTGGLEISIRGMPASYTLILIDG
                     VRQGGSSDVTPNGFSAMNTGFMPPLAAIERIEVIRGPMSTLYGSDAMGGVVNIITRKN
                     ADKWLSSVNAGLNLQESNKWGNSSQFNFWSSGPLVDDSVSLQVRGSTQQRQGSSVTSL
                     SDTAATRIPYPTESQNYNLGARLDWKASEQDVLWFDMDTTRQRYDNRDGQLGSLTGGY
                     DRTLRYERNKISAGYDHTFTFGTWKSYLNWNETENKGRELVRSVLKRDKWGLAGQPRE
                     LKESNLILNSLLLTPLGESHLVTVGGEFQSSSMKDGVVLASTGETFRQKSWSVFAEDE
                     WHLTDALALTAGSRYEHHEQFGGHFSPRAYLVWDVADAWTLKGGVTTGYKAPRMGQLH
                     KGISGVSGQGKTNLLGNPDLKPEESVSYEAGVYYDNPAGMNANVTGFMTDFSNKIVSY
                     SINDNTNSYVNSGKARLHGVEFAGTLPLWSEDVTLSLNYTWTRSEQRDGDNKGAPLSY
                     TPEHMVNAKLNWQITEEVASWLGARYRGKTPRFTQNYSSLSAVQKKVYDEKGEYLKAW
                     TVVDAGLSWKMTDALTLNAAVNNLLNKDYSDVSLFSAGKSTLYAGDYFQTGSSTTGYV
                     IPERNYWMSLNYQF"
     gene            complement(2397..2547)
                     /locus_tag="ACHKTW_004851"
                     /pseudo
     CDS             complement(2397..2547)
                     /locus_tag="ACHKTW_004851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066419.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            3040..3282
                     /locus_tag="ACHKTW_004852"
     CDS             3040..3282
                     /locus_tag="ACHKTW_004852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6465727.1"
                     /translation="MKDEIHSTSINPDVVEQFLALMNTHCKSSEEALAMLSTLCYNLM
                     VTCDIHSMDSQMLNGHGISMQLQGIDIAERPGKNIH"
     gene            3370..3561
                     /locus_tag="ACHKTW_004853"
     CDS             3370..3561
                     /locus_tag="ACHKTW_004853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6465728.1"
                     /translation="MKKTKTHTGLLASKDKTRRVSLYETPTAWCIRGQECYSKSTGRR
                     CGSHDSLSRLRLDSIKPVE"
     gene            3573..3941
                     /locus_tag="ACHKTW_004854"
     CDS             3573..3941
                     /locus_tag="ACHKTW_004854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001456887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6465729.1"
                     /translation="MKDNNTTATPEVWKMRFLANGTSGTWFYTDDIAVTDAWLRAHPD
                     DVEVIPLCRIPPRQHSHEQHIEELRAALARMLHMHHMMLETTHYRENITDARCIREMH
                     EAPLQALNILARYPHKKDEV"
     gene            3945..4160
                     /locus_tag="ACHKTW_004855"
     CDS             3945..4160
                     /locus_tag="ACHKTW_004855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001622039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6465730.1"
                     /translation="MWLLIFWLSSPFNVSSGDARQPTLAVHSQAFHTERACRDALRAI
                     KKENVGEFSLRGVCVSDGNTKEEAESS"
     gene            4157..4429
                     /locus_tag="ACHKTW_004856"
     CDS             4157..4429
                     /locus_tag="ACHKTW_004856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neurotensin receptor R8"
                     /protein_id="HFO6465731.1"
                     /translation="MTDITDRHTLRRMSWSELFTAAQEAEFQRDYERARIVWSFALHV
                     ATTTINKNLAIAHIRRCDTLLHKSKTVPGNNTGGRSVCLRPQHPRR"
     gene            complement(4738..5569)
                     /locus_tag="ACHKTW_004857"
                     /pseudo
     CDS             complement(4738..5569)
                     /locus_tag="ACHKTW_004857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000555401.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            5627..5812
                     /locus_tag="ACHKTW_004858"
                     /pseudo
     CDS             5627..5812
                     /locus_tag="ACHKTW_004858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001362703.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
BASE COUNT         1614 a         1429 c         1499 g         1503 t
ORIGIN      
        1 tttttgtaaa aggcaaatat aaattattct cattattgtt tgtatttgtg tattgtcttg
       61 ccggttaaca tgatcggaga ttagtaatat gcgaataacc actctggctt ccgtagtcat
      121 tccctgtctc ggattttcag ccagcagcat agctgctgca gaggatgtga tgattgtctc
      181 ggcatccggc tatgagaaaa agctgactaa cgcagccgcc agtgtttctg tgattagcca
      241 ggaggaattg cagtccagcc agtaccacga tctggcggag gctctgagat cagtagaggg
      301 tgtggatgtt gaaagtggta cgggtaaaac cggtgggctg gaaatcagca tccgaggaat
      361 gccagccagt tacacgctga tactgattga tggtgttcgt cagggcggaa gcagtgacgt
      421 gactcccaac ggtttttctg ccatgaatac cgggttcatg ccccctctgg ccgccattga
      481 gcgtattgag gttatcaggg ggccgatgtc cacactgtat ggctctgatg cgatgggcgg
      541 tgtggtgaat atcattacca gaaagaatgc agacaaatgg ctctcttccg tcaatgcagg
      601 gctgaatctg caggaaagca acaaatgggg taacagcagc cagtttaatt tctggagcag
      661 tggtcccctt gtggatgatt ctgtcagcct gcaggtacgc ggtagcacac aacagcgtca
      721 gggctcatcg gtcacatcac tgagcgatac agcagccacg cgtattcctt atcccacgga
      781 gtcacagaat tataatcttg gtgcacgtct tgactggaag gcgtcggagc aggatgtgct
      841 ctggtttgat atggatacca cccggcagcg ttatgataac cgggatgggc aactggggag
      901 tctgacgggg ggatatgacc ggaccctgcg ctatgagcga aacaaaattt cagctggcta
      961 tgatcatact ttcaccttcg gaacatggaa atcgtatctg aactggaacg agacagaaaa
     1021 taaaggtcgt gagctcgtac gcagtgtact gaagcgcgac aaatgggggc ttgccggtca
     1081 gccgcgggag cttaaggaat cgaaccttat cctgaattca ttactgctta cccctctggg
     1141 agaatctcat ctggttacgg tggggggcga gtttcagagc tcgtccatga aagacggagt
     1201 tgtccttgcc agcacaggtg aaactttccg gcagaaaagc tggtcggtat ttgctgagga
     1261 tgagtggcat ctcacggatg cacttgcgct gactgcgggc agccgctatg aacatcatga
     1321 gcaattcggg ggacacttca gtccgcgtgc atatctggtc tgggatgtgg cagatgcctg
     1381 gacgctgaaa ggcggtgtga ccacgggata taaggcaccc agaatggggc agctacataa
     1441 agggattagt ggtgtgtccg ggcagggaaa aacaaatcta cttggtaacc ccgacctgaa
     1501 gccggaagag agcgtcagtt atgaggctgg ggtgtattac gataaccccg ccggtatgaa
     1561 tgccaatgtc acaggtttta tgactgactt ctccaacaag attgtctctt attccataaa
     1621 tgataacacc aatagctatg taaacagcgg aaaggcccgg ttgcacggtg tggaatttgc
     1681 cggcacattg ccgctgtggt cagaggatgt cacgctgtca ctgaattaca cctggacccg
     1741 aagtgaacaa cgtgatggtg ataacaaagg tgcgccgctg agttataccc ctgaacacat
     1801 ggtgaatgcg aaactgaact ggcagatcac cgaagaggtg gcatcatggc tgggtgcccg
     1861 ttatcgcggg aaaacaccac gtttcaccca gaattattcg tcactgagcg ctgtacagaa
     1921 gaaagtgtat gatgagaaag gagaatacct gaaagcctgg acggtggtgg atgcaggtct
     1981 gtcgtggaag atgacggatg ccctgacgct gaatgctgcg gtgaataacc tgctcaacaa
     2041 ggattacagt gacgtgagcc tgttcagtgc cggtaagagt acgctgtatg ccggtgatta
     2101 cttccagacg ggatcatcaa caacaggata tgtgatacct gagcgaaatt actggatgtc
     2161 gctgaactat cagttctgat aataacaaaa cgctatcact gacggtagaa tacgttgcca
     2221 ctgcaactcc tggcggaaca gtggcaacgt attaggttaa gtgcatttcc gatccgctaa
     2281 tgagatttcg ttaccaacaa ctaatatcgt cacaggaaat gcacggatta tttttaactt
     2341 atcatttaca tacttgtcca gagtgtaagc gcaccgtgaa ggacgagggg taaaaattag
     2401 tttacagaga gagtgacgtt ccagggcaac aactctttca tgcggttggc aggccaggtg
     2461 ttgattacac tgatcacgtg gcgtagccac gtttccggct cgattccgtt aagttttgca
     2521 gctaccgatc aggctgtaca tcactgccgc actatcgctc gtcatctcaa agtccggtcc
     2581 gtcagcagga aggtatcatt ctctcccgcc atttttccgg gggcccggtc agataagtcc
     2641 ctttgtctat cgctgactcc tggctcataa cccggttagc agaatgcagg gtcaccacct
     2701 cgccacgacc gagtccggat aagtcaattg caccttctca atcgccattt tgtcacaatt
     2761 aagtttgata aagcaggtca cctctgcact gaatctgctc cagtgcctca ttggtctccg
     2821 tccccccctt taaactgccg gataagactg ctgcacagcg ccttccccgg aatcaaatca
     2881 ctttccctgt ccgccacaat ccgcttcatg ccacctcgta ttttgtttca gagaccggaa
     2941 tctgccagaa agtgactacg cgtgcacata tcggttttct gggttacatg catcgtgtta
     3001 ttttctgaaa tgcaaaataa taaaaaacaa ggacacataa tgaaggatga aatacacagt
     3061 accagcatca acccggatgt tgttgagcag tttctggccc ttatgaacac acattgtaaa
     3121 tcctcagaag aggcgctcgc catgttgagt acgctttgtt acaacctgat ggtgacatgt
     3181 gatatccatt ccatggacag ccagatgctc aatgggcatg ggatatccat gcaacttcag
     3241 gggattgata ttgccgaaag gccaggtaag aatattcact aacctgaaca gggtctgaaa
     3301 acgctgtctt ttcaggcctc tgcaggcaaa aactgatgac aacaaaattt ctgcatttgt
     3361 accccaccga tgaaaaagac aaaaactcac accggacttc tagccagtaa agataaaacc
     3421 cgacgtgtca gcctctacga aacgccaacg gcatggtgta tcagggggca ggagtgctac
     3481 agcaaaagta caggacgccg ctgtggcagc cacgacagtc tgagcagact gcgcctggac
     3541 agcattaagc ctgttgaatg agtggtggca ccatgaaaga taacaacaca accgccaccc
     3601 cagaagtctg gaaaatgcgt tttctggcca acgggacatc cggcacatgg ttttacactg
     3661 acgacattgc agtcactgac gcgtggcttc gcgcgcaccc ggatgatgtg gaggttatcc
     3721 ctctctgccg gataccaccc cgtcagcatt cccatgaaca acatattgaa gaactccgcg
     3781 cagccctggc ccggatgctc catatgcatc atatgatgct ggaaaccacg cattacaggg
     3841 aaaacatcac cgatgcccgt tgtatccgtg aaatgcatga agctccatta caggcgctca
     3901 atattctggc cagatacccg cataaaaagg acgaggtgtg acgcatgtgg ctactgattt
     3961 tctggctttc atctcccttt aatgtctctt ccggtgatgc ccggcagccc acactggcgg
     4021 tgcacagcca ggcatttcat accgaacgag cctgtcgcga tgcattgcgc gccattaaaa
     4081 aagagaatgt tggcgaattt tctctgcggg gcgtttgtgt ttctgacgga aatacgaagg
     4141 aggaagctga gagctcatga cggatattac tgacagacat acacttcgcc gtatgtcctg
     4201 gtcggaactc ttcacggcag cacaggaagc ggaatttcag cgtgattacg agcgtgccag
     4261 aatagtgtgg tcattcgcct tacatgttgc aacaaccacg attaacaaaa atctggccat
     4321 agctcatatt cgacgctgcg atactttgtt gcataaatca aaaacagtac ccggaaacaa
     4381 cacggggggc cggtcagtgt gcctgagacc tcaacatccc cggagatgac tgacattccg
     4441 gactgacgca gttgctgcat atctgtggca atacgttgta tccagccaga caggctatcc
     4501 cgcgtagtga caattaaccc gctttcctgc accagcttca gccgggccgt cggggatttt
     4561 aacagccgga aatatcagta aaacaacatt tcattatgcc atggtgtacg cggccagtgt
     4621 acgcttactt aaggaatgag ggataaattg gatattatta cttcactttg cttttttatg
     4681 agattcatca cataagtggg gttttactgt taaataatct gaatttattt gatatccgta
     4741 aagcagatgt ctcagttcac tgtttccaac ttttgttatt cctctggggc gaatgctgtt
     4801 tccgctggaa tattcacctg gcaccagtcc cagaaaagcc atgagctgtt caggattgga
     4861 aaatcgcgaa aaatcaccca gttcagcaac cagggttatt gcgattagct gaccaacacc
     4921 tttaagagcc tgcaactggc agaccagatt gcacagagat cactcaggta acaggcgaga
     4981 tatttcctga tcgagttgaa gtatgcgatc ttcaatctgc tccagattct gacaataatg
     5041 ttgaaagcaa tctgttgaga tagcagacca aatgattgat taactaacca ggttcgatgt
     5101 ttgccggttc agtgcttcct ggcataacgt ttgtctgttc gtgtcacgtt ttgcagcggc
     5161 acgggctctg attaaatccc gcatagcttc atgagtcagc tcagggagcc agactgaggg
     5221 aacctcacca tccctcagta acctggcgag agaaattacg tcccgatggt catttttgat
     5281 cctgtctgcc ggtgatttgg gtatacggga agggggcaat aaccagacat tttatattca
     5341 tcactgtgag ttgatgatat agcccaaacc cacagggacc accttcatag caggaaagaa
     5401 tgtttccgta aggtccctgc aatttctgac ataatcgatg tacagccaag gcttcgttgt
     5461 taatcgtacc ataataacga atttcgccaa gccattcagg tgtagcaatc gccaaggttt
     5521 ctttatgaac aaccagcccg acgtaagctg caatattttc cgaatccatg atgacctctg
     5581 gaatatccca ccaatgctat agtgagtata taaaggtcat atcatctacg tgttcttcgg
     5641 caacgatcac agtggagagc gtggagcgct actgtacggg ctgattggca cctgccgact
     5701 gaacagtatc gacccagaag cgtatttgcg ccatatcctg ggcgtactac tggaatggcc
     5761 ttccaaccgt gttgacgaac gctcgccatg gaatgtagta tattaccaat aaataaccgt
     5821 caatacggca ctcggttaac gcttacaaat aagaatagac aggacaaatg taaactacat
     5881 ttgcatattc tgtataacat tatcacacaa gagaatgaca ccaacatttc tctggtgtat
     5941 aattcttttt tgtgctcgta aaattaatgt tgcggagtaa tatacctcct ttgttatatt
     6001 taaatcattc aataaaacgc ctaattttgc agggctatta ctgga
//
LOCUS       DAZFQD010000207         5967 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.207, whole genome shotgun sequence.
ACCESSION   DAZFQD010000207 DAZFQD010000000
VERSION     DAZFQD010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5967
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.207"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(97..858)
                     /gene="kduD"
                     /locus_tag="ACHKTW_004859"
     CDS             complement(97..858)
                     /gene="kduD"
                     /locus_tag="ACHKTW_004859"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HFO6465732.1"
                     /translation="MILSAFSLEGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIKQVTALGRRFLSLTADLRKIDGIPALLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPIVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(888..1724)
                     /gene="kduI"
                     /locus_tag="ACHKTW_004860"
     CDS             complement(888..1724)
                     /gene="kduI"
                     /locus_tag="ACHKTW_004860"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HFO6465733.1"
                     /translation="MDVRQSIHSAHAKTLDTQGLRNEFLVEEVFVADEYTMVYSHIDR
                     IIVGGIMPITKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFDDMDHVAVKD
                     LR"
     gene            complement(2011..3192)
                     /gene="yqeF"
                     /locus_tag="ACHKTW_004861"
     CDS             complement(2011..3192)
                     /gene="yqeF"
                     /locus_tag="ACHKTW_004861"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HFO6465734.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMIQSNGQTLVVDTDEQ
                     PRTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            3447..4676
                     /gene="yqeG"
                     /locus_tag="ACHKTW_004862"
     CDS             3447..4676
                     /gene="yqeG"
                     /locus_tag="ACHKTW_004862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFO6465735.1"
                     /translation="MSNIWSKEETLWSFALYGTAVGAGTLFLPIQLGSAGAVVLFITA
                     LVAWPLTYWPHKALCQFILSSKTSAGEGITGAVTHYYGKKIGNLITTLYFIAFFVVVL
                     IYAVAITNSLTEQLAKHMVIDLRIRMLVSLGVVLILNLIFLMGRHATIRVMGFLVFPL
                     IAYFLFLSIYLVGSWQPDLLTTQVEFNQNTLHQIWISIPVMVFAFSHTPIISTFAIDR
                     REKYGEHAMDKCKKIMKVAYLIICISVLFFVFSCLLSIPPSYIEAAKEEGVTILSALS
                     MLPNAPAWLSISGIIVAVVAMSKSFLGTYFGVIEGATEVVKTTLQQVGVKKSRAFNRA
                     LSIMLVSLITFIVCCINPNAISMIYAISGPLIAMILFIMPTLSTYLIPALKPWRSIGN
                     LITLIVGILCVSVMFFS"
     gene            5136..5767
                     /gene="yqeH"
                     /locus_tag="ACHKTW_004863"
                     /pseudo
     CDS             5136..5767
                     /gene="yqeH"
                     /locus_tag="ACHKTW_004863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417323.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
BASE COUNT         1654 a         1427 c         1256 g         1630 t
ORIGIN      
        1 atatggatta tttcataacc atgatatgga ttatgatgat ctacaggtat aaaaaaccct
       61 gccatgcggc agggtcataa aagtaagaag aatgaattaa cgcgccagcc aaccgccatc
      121 cacggcaatg gtataaccat tcacataatc tgaagcgctg gaggcaagga acactatcgg
      181 ccccatcagg tcactcggca gtccccaacg accagctgga atgcggtcga gaatttccgc
      241 gctacgttgt tcatctgccc gtagttgttg agtattgttg gtcgccatgt aacccggagc
      301 tatcgcatta acattaatgt tgtgtttagc ccattcgttc gccatcaatc gcgtcacacc
      361 catcacgccg ctttttgatg cggtataaga aggcacacgg atcccgccct ggaaggagag
      421 cattgacgcg atattgataa tcttgccgcc attgccttgc gcgataaagt gtttcgccgc
      481 tgcctgagac atgaagaata cgctcttgat attcaggttc atgacatcgt cccagtcctt
      541 ttcgctgaac tcgagagcat cttcgcggcg aatcaatccg gcgttattca ccaagatatc
      601 aatatgacca aactccgcta ccgcgcgatc cagcagtgct ggaataccat caatctttcg
      661 cagatcggcg gtcaggctta aaaaacgacg ccccagcgcc gtgacctgct tgatggtttc
      721 agtaggttca acgatgttaa tgccaacaat gtcacagccc gcttgcgcca gccccaacgc
      781 catcccctgg cccagcccag tatcacagcc agtgacgacc gcaactttac cttcgagaga
      841 aaatgcactt aaaatcataa caatacctta ttcgtttatg cccacaacta gcgcaaatct
      901 ttaacggcca catggtccat atcatcaaag acctggtttt caccgaccat gccccagata
      961 aaggtataag ctttggttcc gacgccggaa tgaatcgacc agctcgggga gatcaccgcc
     1021 tgctcgttat gcatcacaat atgacgcgtt tcttgcggct gccccatcat gtggaaaacg
     1081 caggcgtcat catccatatt gaaatagaaa taaacttcca tccggcgctc gtgagtgtga
     1141 cacggcatgg tgttccacaa gttacctgga gccagctccg tcagccccat actcaattgg
     1201 caggtttcca gcacatccgg gacaaaatat ttgttaattg tgcgacggtt actggtgagg
     1261 ttatcgccta acgtgactgg agatacttcg tccggtgtga cttttttggt gggatacgtc
     1321 gtatgcgcgg gtgcgcaatt gtaataaaac tttgccggag tggcggtatc aatactggca
     1381 aagacaactt cttttgcacc tttacccaca tacagggcgt cgcggtgacc gatttcatag
     1441 cattggccat cgacagtaat cgtaccggca ccgccaatat tgataacacc taactcgcga
     1501 cgttcaagga aatagcttac gccgagttgt ttaccaactt ccccgccaac ggaaaccgtt
     1561 ttagttatcg gcataatgcc gccaacaata attcggtcaa tgtggctgta aaccatggtg
     1621 tactcatcgg cgacaaatac ctcttcaacc aaaaattcat tgcgcagccc ctgggtatcc
     1681 agcgtttttg cgtgcgcact gtggatgctc tgtcttacgt ccacatcaac ctccgataat
     1741 cagtgaagcg tgaaaataga aaacgatagt gaaacactgt ttcgttttct ctataaaggg
     1801 atgatatgcc catgggctag ggttttcaat taaaaataaa acaatgtttc atttttaagt
     1861 taaggattaa aaaaagtgca gtgtcgatca cgaatatgtc atgagaatgg aaaaatgagg
     1921 gagcccagaa aatgtatgtc gacttgccag ccatcattat tcaacagatt aatgacggca
     1981 gatatttatc ggtaaggagg aaatgcagag ctactcgtca cgttcaatgg tcaatgccac
     2041 gccctggccc ccgccgatac aaagcgttgc cagccctttg cgggcattac gtttcaccat
     2101 ttcatgaacc agagaaacca ggattcggca accggaagcg cctatcgggt gaccgagtgc
     2161 gatcgcgcca ccattgacat tgacccgacg ctcatcccac tcaagcatct tgccaaccga
     2221 aagcgcctgt gcagcaaacg cttcattagc ctcgataaga tcgacatcag ccaactgcca
     2281 gcctacacgc tccaggcaac ggcgggtcgc atacaccggc gcaattccca tcaatgccgg
     2341 atctacacca acgctggcaa atgcgcggat gcgggccagc acgggtaaat tcaacgctcg
     2401 tgctttggct tcgctcatca tcattaccgc agctgcgcca tcgtttatgg atgatgcatt
     2461 ccccgcggtc acagaaccga gactatcaaa tgaaggattt aaacgggcta aaccttctgc
     2521 actggcgtca gtgcgtggct gttcatcggt atcaacaacc aacgtctgac cgttactttg
     2581 gatcattacc gggacgatct catctttaaa tcgtccggcg tcaatcgccg ctcgcgcttt
     2641 ttgttgcgag ctaagtgcgt aagcatcctg caactgacgg ctgatgccat attcgcgagc
     2701 cagattttcg gcggtgacac caatatgata atcattgaag gcatcccaca acccatcatg
     2761 caccagactg tcaaccaact ggctattgcc aagctgtgca ccggtgcggc tatcagtcag
     2821 aacatgtggt gcgcggctca tgttttcctg gccaccggcg atgacaatat cagcctcgcc
     2881 acactgtatc gcctgagtag ccagatgcag tgctttaagc ccagaaccgc aaacgtcatt
     2941 aatagtgatt gcagaaacgc tattaggaag accaccttta atagccgatt gccttgccgg
     3001 attctgccct gcccctgcag tcaacacctg accaagaatt acttcatcca ccgcatatgc
     3061 aggaacgccg gtacgttcta ttaacgcttt cacgaccaga ctaccaagtt ccacggcgga
     3121 atgacccgct aacgcaccgc gaaagcagcc gataggtgtc cgtaacgccc cgacaatcac
     3181 aacgtctttc atcacaacct cgcaccaatt aacagtgcaa tagtaaatga ttgttaatta
     3241 cagttatctt aattgtttaa aaaaagtgat ttttatcaca aaggaaatat gcctgagcag
     3301 tagtcagagt cataactggc acgtaaggtt tgcaaccact aacccaccaa tagaggggta
     3361 gatagggcgt taatctccca tacttaacct ggtttatggt aaattgccct ccattttgtt
     3421 taatttgtag atgatacgtt cagataatgt ctaatatttg gtctaaagaa gaaactctgt
     3481 ggagtttcgc gctctacggc acagccgttg gtgcaggcac gctcttcctt cctattcagt
     3541 taggttcggc aggggctgtg gtcctgttta ttactgctct ggtcgcctgg cctttaacat
     3601 attggccaca taaagcctta tgccagttca tcctctcatc gaaaacatca gcaggtgaag
     3661 ggataacggg cgcagtaaca cactactatg gcaagaagat tggtaatctg attaccacgc
     3721 tgtacttcat cgcctttttt gtcgtcgtgt tgatatatgc agtggcaatt accaactcac
     3781 ttacggaaca gctggcaaag catatggtta ttgatcttcg catccgtatg ttggtgagtc
     3841 tgggtgttgt attaatcctg aatctcattt ttctgatggg acgccatgca accattcggg
     3901 taatgggttt tttggtattc ccattgattg cctatttctt atttctttcc atttaccttg
     3961 tcggtagttg gcaacctgat ctattaacga cccaggtaga gttcaatcag aatacccttc
     4021 accagatatg gatatcgatt cccgtgatgg ttttcgcctt tagccatacg cccattattt
     4081 ctacgtttgc catagacaga cgtgaaaaat atggcgaaca cgctatggat aaatgcaaaa
     4141 aaattatgaa agtcgcttat ctcatcatct gcataagtgt actgttcttt gtctttagct
     4201 gcctgctttc tattccacct tcgtatattg aagcggctaa agaagaaggg gtcaccattt
     4261 tatcggcgct ttctatgctg ccgaacgccc cagcatggct gtcaatttcc gggattattg
     4321 tcgcagtagt tgcgatgtcg aaatcattcc tgggtacgta ctttggcgtt attgaaggtg
     4381 ccacggaggt cgtcaaaaca acactacagc aggttggtgt aaagaaaagt cgtgcattta
     4441 accgcgcact atcaattatg ttggtatcgc tgatcacctt cattgtttgt tgcattaacc
     4501 cgaacgcgat ttcgatgatt tacgcgatca gcggcccgct cattgccatg atacttttca
     4561 tcatgcctac gttgtcaacg tatctgatcc cggcgcttaa accctggcgt tccatcggaa
     4621 atctgattac gctgatcgtg ggtatcctgt gcgtatcggt aatgttcttt agctgatatc
     4681 cattgtgaat ggttcaaaat tgctacaatt ttgaaccatt ccagttttca taatgttcat
     4741 tcgttattat tccgcactat cacttccctc atcatatcct agtattatca gttagttaaa
     4801 cacacaaaga gatctgaaca tcttaaataa gactatttaa gatgcataac ttagattcgc
     4861 aagatatctt ccggtcattc aaaaacaatt tgcgatatag atattctcat accatgagat
     4921 agttatctga accaccctca accaggtaag attaaatcag tgaaacataa tcatatttaa
     4981 taacgagaaa tgcattttaa aatgtcttaa tcagaattat agcaaataca gattaatcgc
     5041 aatttttccc agcaaaatta caaaatcatg gtgtattgac atcatcaatc accaatgaaa
     5101 ttatgcaatt atatacggat agggaggttc ttaacatggg gctttgtagt cgttataaaa
     5161 gtcttacatg caatagttgc tcaatgcatt gccaaataat gccagaagag tcaccgcgtt
     5221 tgcagtactg cgcaaactcg tgtttttgta tgtggcccga agaaagttca tattttaatc
     5281 gtggtgtggt agaaggtatc ttaacaaaaa accacaatgc gagattaagc ggatatattt
     5341 ttgtcgattt ttcagtaagt tttttacgtc tattccttga aaaagactgg attgactatc
     5401 ttgcaagtac tgacatgggg attgttttag tcagcgaccg caatatgcat cgttagctaa
     5461 ttactggcga aaacacaact cagcaatatc tgctgtcata tataatgatg atggtcttga
     5521 tgttgccaat gaaaagatca gacaactatt tattggtcgt tatttatcat ttaccagagg
     5581 taacacatta actcagatgg aattcaccat aatggggtat atggtttctg gttataatcc
     5641 atatcagatt gctgaagttc tggatatgga tatccgtagc atctacgcgt acaagcaacg
     5701 aatcgaaaag agaatgggtg gtaaaataaa cgaattattt attcgttcac attcggtcca
     5761 acattgatac tcaacgacca gccagaatca tactctgttt atacgggcat ttattgaaat
     5821 gtccgtattt tataaatagg ccgtcccctc aagagtaaac accatagtat ttaaaaccat
     5881 cacacgaaaa attcagaagc attacgaaat atgaatttac aaacaatagt ggcataaatg
     5941 ttaaccatgt taatttacgt aaagttt
//
LOCUS       DAZFQD010000208         5965 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.208, whole genome shotgun sequence.
ACCESSION   DAZFQD010000208 DAZFQD010000000
VERSION     DAZFQD010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5965
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.208"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..1182)
                     /locus_tag="ACHKTW_004864"
     CDS             complement(<1..1182)
                     /locus_tag="ACHKTW_004864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFO6465736.1"
                     /translation="MKKAIAYMRFSSPGQMSGDSLNRQRRLIAEWLKVNSDYYLDTIT
                     YEDLGLSAFKGKHAQSGAFSEFLDAIEHGYILPGTTLLVESLDRLSREKVGEAIERLK
                     LILNHGIDVITLCDNTVYNIDSLNEPYSLIKAILIAQRANEESEIKSSRVKLSWKKKR
                     QDALESGTIMTASCPRWLSLDDKRTAFVPDPDRVKTIELIFKLRMERRSLNAIAKYLN
                     DHAVKNFSGKESAWGPSVIEKLLANKALIGICVPSYRARGKGISEIAGYYPRVISDDL
                     FYAVQEIRLAPFGISNSSKNPMLINLLRTVMKCEACGNTMIVHAVSGSLHGYYVCPMR
                     RLHRCDRPSIKRDLVDYNIINELLFNCSKIQPVENKKDANETLELKIIELQMKINNLI
                     VA"
     gene            complement(1240..1389)
                     /gene="ylcJ"
                     /locus_tag="ACHKTW_004865"
     CDS             complement(1240..1389)
                     /gene="ylcJ"
                     /locus_tag="ACHKTW_004865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001299444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YlcJ"
                     /protein_id="HFO6465737.1"
                     /translation="MSLVLCFLLMSLFFMYSFVLSRLWRKKIAIRLLLYIQDNVTLIV
                     FLNKN"
     gene            complement(1437..1769)
                     /gene="emrE"
                     /locus_tag="ACHKTW_004866"
     CDS             complement(1437..1769)
                     /gene="emrE"
                     /locus_tag="ACHKTW_004866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter EmrE"
                     /protein_id="HFO6465738.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGLFGQRLDLPAIIGMMLICAGVLVIN
                     LLSRSTPH"
     gene            complement(1837..2139)
                     /locus_tag="ACHKTW_004867"
     CDS             complement(1837..2139)
                     /locus_tag="ACHKTW_004867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ren"
                     /protein_id="HFO6465739.1"
                     /translation="MTGKEAIIHYLGTHKSFCAQDVAAVTGATVTSINQAAAKMARAG
                     ILVVDGKVWRTVYYRFATREEREGKVSTNLIFKECRQSAAMKRVLRVYKRTSMGTQ"
     gene            complement(2136..2837)
                     /locus_tag="ACHKTW_004868"
     CDS             complement(2136..2837)
                     /locus_tag="ACHKTW_004868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HFO6465740.1"
                     /translation="MKNIAAQMVNFDREQMRRIANNMPEQYDEKPQVQQVAQIINGVF
                     SQLLATFPASLANRDQNELNEIRRQWVLAFRENGITTMEQVNAGMRVARRQNRPFLPS
                     PGQFVAWCREEASVNAGLPNVSELVDMVYEYCRKRGLYPDAESYPWKSNAHYWLVTNL
                     YQNMRANALTDAELRRKAADELTCMTARINRGETIPEPVKQLPVMGGRPLNRAQALAK
                     IAEIKAKFGLKGASV"
     gene            complement(2834..3853)
                     /locus_tag="ACHKTW_004869"
     CDS             complement(2834..3853)
                     /locus_tag="ACHKTW_004869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFO6465741.1"
                     /translation="MTGSEMAKVGLREQNRLSGANRNTLIAGGIMANTAEIFNFPVPD
                     AAQKERRVADLDDGYTRIANELLEAVMLAGLTQHQLLVFLAVMRKTYGFNKKLDWVSN
                     EQLSELTGILPHKCSAAKSVLVKRGIFIQSGRNIGINNVVSEWSTLPESGKKNKVYLK
                     EVNLPESGKKSLPKSGKGVYPNQVNTKDKLTKDNIKPFSSENSGESSDQPENDFPVEK
                     PDAAIQSGSKWGTAEDLTAAEWMFDMVKTIAPSARKPNFAGWANDIRLMRERDGRNHR
                     DMCVLFRWACQDNFWSGNVLSPAKLRDKWTQLEINRNKQQAGVTAGKSKLDLTNTDWI
                     YGVDL"
     gene            complement(3850..4389)
                     /locus_tag="ACHKTW_004870"
     CDS             complement(3850..4389)
                     /locus_tag="ACHKTW_004870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001182892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT domain-containing protein"
                     /protein_id="HFO6465742.1"
                     /translation="MQPLTYQQTSGFSPTAVINRSQTKQAPGHEKIRDAVRAWSAADN
                     QDVVAALIVNEYREQGGGTIDFPDDVSRARQKLFRFLDNKFDSEKYRNNVRELTPAIL
                     AVLPLEYRGYLVEQDSFMARLAEMEKELSEAKQAVILNAPRHQKLKEISEGIVSMFRV
                     DPDLAGPLMAMVTTMLGAI"
     gene            complement(4420..4647)
                     /locus_tag="ACHKTW_004871"
     CDS             complement(4420..4647)
                     /locus_tag="ACHKTW_004871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004201115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO6465743.1"
                     /translation="MTNQTIQLAISITGSQKRLADLCGVAQPTVWRWLHGGGIDARYV
                     MKIVSATGGKIKPADIRPDLAPLFNASNSAA"
     gene            4758..5450
                     /locus_tag="ACHKTW_004872"
     CDS             4758..5450
                     /locus_tag="ACHKTW_004872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001337214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFO6465744.1"
                     /translation="MKKETLADRLNLAMEQSGMSQGALAKASGVAQPTIWRLTSGNAR
                     GSTKIVEIANALGVRTEWLSSGIGPMRNDGQQLGKPTANHPKYFKIDVLDIEVSAGPG
                     VINREFVEVLRSVEYSFDDARHMFDGRKAENIRIINVRGDSMSGTIEPGDLLFVDITV
                     KSFDGDGIYAFLYDDTAHVKRLQMMKDKLLVISDNKSYSPWDPIEKDEMNRVFIFGKV
                     IGSMPQTYRKHG"
     gene            5605..>5965
                     /locus_tag="ACHKTW_004873"
     CDS             5605..>5965
                     /locus_tag="ACHKTW_004873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001750114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6465745.1"
                     /translation="MNVRDLAPALLSLSDALEEAGKTLYGNKTVVSVKVNASFKAGSF
                     GIDLVASSTSWFKQAVDFLSGDSATAAANLIAFIGLCPGSKEKICKGLIQLIKWIGPR
                     KIKKLHNLPDSNIEVFVD"
BASE COUNT         1591 a         1411 c         1236 g         1727 t
ORIGIN      
        1 tgcaacgatt aaattattaa ttttcatctg aagctcaata atttttaact ctaaagtttc
       61 attagcatct ttcttgtttt caactggttg aattttgcta caattaaaaa gcaattcatt
      121 aatgatatta taatcaacca aatctctttt tattgatggc ctgtcacatc gatgtaatct
      181 tctcatcgga caaacataat agccatgcaa acttccagat accgcatgaa caatcatggt
      241 attaccacaa gcctcacact tcataactgt tcgaagtaga tttattagca taggattctt
      301 gctactattg ctaataccaa aaggtgccaa ccgaatttcc tgtacagcgt aaaacaaatc
      361 atctgatatg actctgggat aatagccagc gatttcactt atccctttcc ctcttgcacg
      421 atatgaaggt acgcatatac ctatcagagc tttattcgct aataattttt caattacaga
      481 aggtccccat gcactttctt ttcctgagaa attctttaca gcatgatcat ttaaatactt
      541 ggctattgca ttcaatgagc gcctttccat cctgagttta aaaattagct caatagtttt
      601 caccctgtcg gggtctggaa caaaagccgt tcttttgtca tctaaggaga gccatctcgg
      661 acaagacgcc gtcataatcg tacctgattc cagtgcatcc tgccgttttt tcttccatga
      721 taatttaacc cgacttgact ttatctcgct ttcttcattt gccctttgtg ctataagtat
      781 ggcttttatt aatgaatatg gctcattcaa agagtcaata ttatagactg tattgtcgca
      841 aagagttata acatcaatac cgtgattcaa aatcaatttc agacgttcaa tcgcttcacc
      901 gactttttct cttgaaagtc tgtccagact ttcaactaac aatgtagttc ctggcaatat
      961 ataaccatgc tctatagcat ctaaaaattc cgaaaaagct cctgattgtg catgctttcc
     1021 tttgaatgca cttaatccta aatcttcata tgttatggta tcaagataat aatcactatt
     1081 tacctttaac cattcagcaa taagtcttct ctgtcggttt aatgagtcac cagacatctg
     1141 acctggtgat gaaaatcgca tatatgctat ggcttttttc atggtgacac ctgctaacgt
     1201 atgcttttat aaaccttagt ggtgggatat aatttttgtt taatttttat ttaaaaagac
     1261 aattaaggtc acattatctt gaatatacaa caataatcgt attgcaattt tcttacgcca
     1321 taatcttgaa agcacaaaag aatacataaa aaataaagac attaacaaaa agcataaaac
     1381 gaggctcata taaatataag agcctccata ttttagtcgt ttagaaacaa attattttaa
     1441 tgtggtgtgc ttcgtgacaa taaattaata accaacacac cggcacaaat caacatcatg
     1501 cctataatgg ctggcaggtc cagccgttgg ccgaaaagtc cccatgacag taagctaatc
     1561 aggacaatac cgactcctga ccagatagca taagcaatcc ctgtaggaat ataagccagc
     1621 gtctgagcta ataaccagaa tgatgcacaa taacaaataa ttgtaccaac agatggccat
     1681 aaccgtgtaa aaccttctga aaacttcatt aaggttgtac caatgacctc tgcaagtatt
     1741 gcaccaccaa gataaatata agggttcata gcatattctt tcctgttcaa actggagaga
     1801 attgtactac agtttgaact caactcacct gtttcatcat tgtgttccca ttgatgttct
     1861 tttatatacc ctcagtaccc gtttcatcgc ggcactctgg cgacactcct taaaaatcag
     1921 attcgtgctc acctttcctt cccgttcttc tctggtagcg aaccggtaat acaccgttcg
     1981 ccagacctta ccatcaacga ccaggattcc tgcccgcgcc attttagccg cagcctgatt
     2041 tatgctggtt acggttgcgc ctgttaccgc ggcaacgtcc tgtgcacaga agctcttatg
     2101 cgtccccagg taatgaataa ttgcctcttt gcccgtcata cacttgctcc tttcagtccg
     2161 aacttagctt taatttctgc gatcttcgcc agagcctgtg cacgatttag aggtctaccg
     2221 cccataacag gaagttgttt tactggttca ggtatcgtct caccacggtt aattcgcgct
     2281 gtcatacagg tcagttcatc ggcagccttg cgccgtaatt ccgcgtcagt cagcgcattg
     2341 gcccgcatgt tctggtacaa gttggtaacc aaccagtaat gcgcgttcga tttccacgga
     2401 taagactctg catccggata caggccacgc ttccggcaat actcgtaaac catatcaacc
     2461 agctcgctga cgtttggcag cccggcgtta acagatgctt cttcccggca ccaggcgaca
     2521 aactgcccgg gtgatggcag gaatggtcga ttctgccgac gggctacgcg cattcctgcg
     2581 ttaacctgtt ccattgtggt gatcccgttt tcccggaaag ccagaaccca ctggcggcgg
     2641 atttcgttca gttcattctg gtcacggtta gccaggctcg ccgggaaagt tgccagtaac
     2701 tggctgaaca caccgttgat gatctgcgct acctgctgta cctgcggctt ttcgtcgtac
     2761 tgttccggca tgttgttggc gatccggcgc atctgctcac ggtcaaagtt aaccatctgt
     2821 gcggcgatgt ttttcataaa tccaccccgt aaatccagtc agtgtttgtc aggtcgagtt
     2881 ttgattttcc ggctgtcacg ccagcctgtt gcttgttacg gttgatttcg agttgggtcc
     2941 acttgtcgcg gagtttggcc ggactcagca cgttaccgga ccagaagttg tcctggcatg
     3001 cccagcggaa cagcacgcac atgtcgcggt ggttacgtcc gtcacgttca cgcatcaggc
     3061 ggatatcgtt agcccaccct gcaaaattcg gttttctggc tgagggcgcg atggtcttca
     3121 ccatgtcaaa catccactct gcggcggtca ggtcttctgc tgtcccccac ttgctgccgc
     3181 tctgaattgc agcatccggt ttctccacag gaaagtcgtt ttctggctgg tcggaggatt
     3241 cgccagaatt ctcggacgaa aaaggtttta tattgtcttt tgttagtttg tcttttgtgt
     3301 ttacctgatt cgggtaaacg cctttacctg atttgggtaa acttttctta cctgattcag
     3361 gtaaatttac ctctttcagg taaactttat ttttcttacc tgattcgggt aatgttgacc
     3421 attcactgac cacattatta atgccgatat tccgcccact ctgaataaaa atcccacgct
     3481 ttaccagaac actttttgca gcagaacact tgtgcggcaa tatcccggtc aactcggaaa
     3541 gttgctcgtt gctcacccaa tccagttttt tattaaagcc atatgttttg cgcatgacag
     3601 ccaggaagac cagaagctgg tgctgtgtta atccggccag catcacagct tccagcaact
     3661 catttgcaat gcgcgtataa ccatcatcga gatctgccac gcgccgctcc ttttgtgccg
     3721 catccggcac tggaaaattg aatatctcag cagtgtttgc cataattcct cccgcaatga
     3781 gtgtgttacg atttgcacct gaaagtcggt tctgttcccg cagaccgact ttcgccattt
     3841 ctgaacctgt catattgccc ccagcatggt ggtgaccatc gccatcagtg gaccagccag
     3901 atccgggtcc acacgaaaca tcgacacaat accttcacta atttccttca gtttctggtg
     3961 gcgtggtgcg ttgagaatga cagcctgttt tgcctcactg agttcctttt ccatttcagc
     4021 caacctagcc ataaagctat cctgctcaac caggtaacca cgatattcca gcggtagtac
     4081 cgccagaatt gccggggtca gttcacgcac gttatttcgg tatttttcag aatcgaattt
     4141 gttatcgagg aagcggaaca gcttctggcg tgcacggctg acatcatcag ggaaatcgat
     4201 ggtgccgcca ccctgctccc gatactcatt cacaatgagt gcggcaacaa catcctgatt
     4261 atctgcagcc gaccaggcgc ggacggcatc acggattttt tcgtggcctg gagcttgttt
     4321 tgtttgagaa cgatttatca ccgcagtcgg gctaaatccg ctagtctgtt ggtatgtaag
     4381 tggttgcata gtcattgcct tatcagttaa cgccgcagtt taggcggcag aattactcgc
     4441 gttaaacaat ggtgcgaggt cgggacgaat atctgctggt ttaatctttc caccagtggc
     4501 tgagacaatt ttcattacat agcgggcatc aattccgcca ccgtgtagcc aacgccaaac
     4561 agtgggttgg gctacaccgc atagatctgc cagtcgtttt tgactacctg taatactgat
     4621 tgcgagttga atggtttgat ttgtcattat caattcctat tggtattgga atgaacaaat
     4681 aatagcaatg cgtattaatc ataacaatag caaaacgtgt tttgaccatc aatacgcaag
     4741 cgtataaatt aaaacttatg aaaaaagaaa ctcttgctga tcgcttaaac ctagcgatgg
     4801 aacaatctgg aatgtctcaa ggcgctcttg caaaggcgtc tggcgtagct caacccacaa
     4861 tctggagact gacaagcggc aacgcgcgcg gctcaacaaa aattgttgaa atagctaatg
     4921 cattgggtgt tcgaacagag tggctctcat caggcatagg cccgatgaga aatgacggtc
     4981 aacaattagg gaagcctact gccaaccatc ccaaatactt caagattgac gttcttgata
     5041 tagaagtgag tgccgggccg ggagtcatca accgtgagtt tgtagaagtt ctacgctcgg
     5101 ttgagtactc gtttgacgat gctcgtcaca tgttcgatgg caggaaggca gaaaatatcc
     5161 gcatcattaa cgtacgcggt gacagcatgt caggaacgat cgaaccaggt gatcttctgt
     5221 tcgttgatat cactgttaaa tctttcgacg gtgatggtat ctatgcgttt ctgtacgacg
     5281 acacagccca tgtaaagcgt ctgcaaatga tgaaggataa gctgctggtt atctctgata
     5341 acaagagcta ctcgccgtgg gacccgatcg agaaagacga gatgaaccgg gtgttcatct
     5401 tcggtaaggt tattgggagc atgccgcaga cgtataggaa gcatgggtag ccatacaata
     5461 gaagttttaa gatttaagac aatatcgtaa attaactgta tatgtgatca ggtgaatgcc
     5521 ataacggatg cgggcaacaa ttcggaaacc aatgatatga agttcaggat agtatacgac
     5581 ggtccggcac tggaaacgca tgaaatgaac gtgcgagacc ttgctcctgc ccttctatca
     5641 ctatcagatg cattagagga agccggtaaa actctctacg gaaacaagac tgttgtttct
     5701 gtaaaagtca atgcatcatt taaagctggc tcatttggaa tagatctggt tgcctcatct
     5761 acatcttggt tcaaacaggc tgttgatttt ttatccggcg attcagcaac agccgctgcg
     5821 aatttgattg cttttattgg actctgtcct ggttctaaag aaaaaatatg caaaggccta
     5881 attcaactga taaaatggat aggcccaagg aaaataaaaa aattacacaa tttgcctgat
     5941 agcaacatcg aagtctttgt tgatg
//
LOCUS       DAZFQD010000209         5927 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.209, whole genome shotgun sequence.
ACCESSION   DAZFQD010000209 DAZFQD010000000
VERSION     DAZFQD010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5927
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.209"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            203..1306
                     /gene="ompC"
                     /locus_tag="ACHKTW_004874"
     CDS             203..1306
                     /gene="ompC"
                     /locus_tag="ACHKTW_004874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HFO6465746.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDDKSVDGDQTYMRLGFKGETQVTDQLTGYGQWEYQIQGNSAENENNSWTRVAFAGLK
                     FQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRNTDFFGL
                     VDGLNFAVQYQGKNGSARGEGMTNNGRDALRQNGDGVGGSITYDYEGFGIGAAVSSSK
                     RTDAQNTAAYIGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGSLGWANKAQNF
                     EAVAQYQFDFGLRPSVAYLQSKGKNLGTIGTRNYDDEDILKYVDVGATYYFNKNMSTY
                     VDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            1418..2473
                     /gene="apbE"
                     /locus_tag="ACHKTW_004875"
     CDS             1418..2473
                     /gene="apbE"
                     /locus_tag="ACHKTW_004875"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HFO6465747.1"
                     /translation="MEISFTRVALLAAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGARTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGQPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPKKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLVSEKN"
     gene            2547..3611
                     /gene="ada"
                     /locus_tag="ACHKTW_004876"
     CDS             2547..3611
                     /gene="ada"
                     /locus_tag="ACHKTW_004876"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HFO6465748.1"
                     /translation="MKKATCLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFHPCKRCQPEKANAQQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLMFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIIIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            3611..4261
                     /gene="alkB"
                     /locus_tag="ACHKTW_004877"
     CDS             3611..4261
                     /gene="alkB"
                     /locus_tag="ACHKTW_004877"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HFO6465749.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILHRFAFNAAEQLIRDINNVASQS
                     PFRQMVTPGGYTMSVAMTNCGHLGWTSHRQGYLYSPIDPQTNKPWPAMPQSFHNLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTTDCRYNLTFRQAGKKE"
     gene            4337..>5927
                     /gene="yojI"
                     /locus_tag="ACHKTW_004878"
     CDS             4337..>5927
                     /gene="yojI"
                     /locus_tag="ACHKTW_004878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HFO6465750.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELVQGIILTIGSAAYLWMLSGKMLLVTAIWMAIT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFSYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGKPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAADQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSEL"
BASE COUNT         1415 a         1497 c         1599 g         1416 t
ORIGIN      
        1 taatcttaaa aagttccctt gcatttacat tttgaaacat ctatagcgat aaatgaaaca
       61 tcttaaaagt tttagtatca tattcgtgtt ggattattct gcatttttgg ggagaatgga
      121 cttgccgact gattaatgag ggttaatcag tatgcagtgg cataaaaaag caaataaagg
      181 catataacag agggttaata acatgaaagt taaagtactg tccctcctgg tcccagctct
      241 gctggtagca ggcgcagcaa acgctgctga agtttacaac aaagacggca acaaattaga
      301 tctgtacggt aaagtagacg gcctgcacta tttctctgac gacaagtctg tagatggcga
      361 ccagacctac atgcgtcttg gcttcaaagg tgaaactcag gttactgacc agctgaccgg
      421 ttacggccag tgggaatatc agattcaggg caacagcgct gaaaacgaaa acaactcctg
      481 gacccgtgtg gcattcgcag gtctgaaatt ccaggatgta ggttctttcg actacggtcg
      541 taactacggc gttgtttacg acgtaacttc ctggaccgac gtactgccag aattcggtgg
      601 tgacacctac ggttctgaca acttcatgca gcagcgtggt aacggctttg cgacctaccg
      661 taacactgac ttcttcggtc tggttgacgg cctgaacttt gctgttcagt accagggcaa
      721 aaacggtagc gctagaggcg aaggcatgac caacaatggt cgcgatgctc tgcgtcagaa
      781 cggcgacggc gttggcggtt ctatcactta tgattacgaa ggcttcggta tcggtgctgc
      841 agtttccagc tccaaacgta ctgatgctca gaacaccgct gcttacatcg gtaacggcga
      901 ccgtgctgaa acctacactg gtggtctgaa atacgatgct aacaacatct acctggctgc
      961 tcagtacacc cagacctaca acgcaactcg cgtaggttcc ctgggttggg cgaacaaagc
     1021 acagaacttc gaagctgttg ctcagtacca gttcgacttc ggtctgcgcc cgtctgtagc
     1081 atacctgcag tctaaaggta aaaacctggg caccatcggt actcgtaact acgacgacga
     1141 agatatcctg aaatatgttg atgttggtgc gacctactac ttcaacaaaa acatgtccac
     1201 ctacgttgac tacaaaatca acctgctgga cgacaaccag ttcactcgtg acgctggcat
     1261 caacactgat aacatcgtag ctctgggcct ggtttaccag ttctaatctc gattgatctc
     1321 gaacaaaggg cctgcgggcc cttttttcat tgttttcagc gtacaaacac agttttttgg
     1381 tgtactcttg cgaccgttcg catgaggata atcacgtatg gaaataagct ttacccgcgt
     1441 ggcactgctg gctgccgcgc tcttctttgt tggttgcgat caaaaaccac aacccgccaa
     1501 aacccacgct actgaagtta ccgttcttga aggcaaaacc atgggtacct tctggcgtgc
     1561 cagcatcccg ggcattgacg ccaaacgcag tgccgaactt aaagaaaaga ttcagaccca
     1621 gctggatgcc gacgatcagc tgctttcgac ctataaaaaa gattccgcgc tgatgcgctt
     1681 taacgactcg caaagtttgt cgccgtggcc ggtaagtgaa gcgatggccg atatcgtcac
     1741 cacctcgctg cgcattggcg caaggaccga tggtgcgatg gatataaccg tcgggccgct
     1801 ggtgaatctg tggggctttg gcccggaaca acagccggtt caaattccga gccaggaaca
     1861 gatcgatgcg atgaaagcca aaaccggctt acagcacctg acggtcatca atcagtcgca
     1921 tcagcaatat ctgcaaaaag acctgccgga tttatatgtc gatctctcca ccgtcggtga
     1981 aggttatgcg gcggatcacc tggcacgctt gatggagcag gaagggattt cccgctatct
     2041 ggtgtcggtg ggcggcgcgc tgaacagccg tggtatgaac ggtgaaggcc agccgtggcg
     2101 ggtagcgatt caaaaaccaa ccgataaaga aaacgcggtt caggctgtgg tggatatcaa
     2161 cggtcatggg attagcacct ctggcagcta ccgtaactat tacgaactgg acggcaaacg
     2221 tctttcccat gttatcgatc cgcaaaccgg gcgtcccatc gaacacaatc tggtatccgt
     2281 gacggtgatt gctccgacgg cgctggaagc cgatgcctgg gatactggct tgatggtact
     2341 cgggccgaag aaagccaaag aggttgttcg ccgggaaggg ctggcggttt atatgatcac
     2401 caaagaaggc gatagcttta aaacctggat gtcaccacag tttaaaagct tccttgtcag
     2461 cgaaaaaaat taaagcgcaa gattgttggt ttttgcgtga tggtgaccgg gcagactaaa
     2521 ggctatcctt aaccagggag ctgattatga aaaaagccac atgcttaact gacgatcaac
     2581 gctggcaatc tgtcttagcc cgcgacccga atgccgacgg cgaattcgtt ttcgccgtgc
     2641 gtaccacagg catcttttgc cgtccgtctt gccgcgccag acatgctttg cgggaaaacg
     2701 tctccttcta cgcaaatgcc agcgaggcac tcgccgctgg ctttcacccc tgcaaacgtt
     2761 gtcagccaga aaaagccaat gcccagcaac atcggttgga taaaatcacc cacgcgtgtc
     2821 gactgctgga acaggaaacg cctgtaacgc tggaagcctt agccgaccag gtggcgatga
     2881 gtccatttca tctacatcgg ttgtttaaag cgactaccgg aatgacgcct aaagcctggc
     2941 aacaggcctg gcgcgctcgc cgtttgcgcg aatcgctggc gaaaggggag agcgtgacga
     3001 cgtctattct taacgccgga ttccccgaca gcagcagtta ctatcgcaaa gctgacgaaa
     3061 cgctgggcat gacggctaaa caattccgtc acggtggcga aaatctggcg gtgcgttacg
     3121 cgctggctga ttgtgagctg ggtcgttgcc tggtggcaga aagcgagcgg gggatttgcg
     3181 cgatattgct gggcgatgat gacgcgacac taatcagcga gttgcagcag atgtttcccg
     3241 ctgccgacaa cgcgcctgcc gatctgatgt ttcagcaaca tgtgcgtgaa gtgatcgcca
     3301 gcctcaatca acgcgatacg ccgctgacgt taccgctgga cattcgcggc actgcttttc
     3361 agcaacaagt ctggcaggca ctgcgcacga taccttgcgg tgaaaccgtc agttatcagc
     3421 aactggctaa cgccatcggc aaaccgaaag cggtacgggc cgttgccagc gcctgtgccg
     3481 ccaacaagct ggctatcata ataccctgtc atcgggtggt ccgtggtgat ggcacacttt
     3541 ccggttaccg ctggggcgtg tcgcgtaaag cgcaactgct gcgccgcgaa gctgaaaatg
     3601 aggagaggta atgttggatc tgtttgccga tgctgaaccg tggcaagagc cactggcggc
     3661 tggagcggta attttgcacc gttttgcttt taacgctgcg gagcaactga tccgcgatat
     3721 taataacgtt gccagccagt cgccgtttcg ccagatggtc acccccgggg gatataccat
     3781 gtcggtggcg atgaccaact gtgggcatct gggctggacg agccatcggc aaggttatct
     3841 ctattcgccc attgatccgc aaacaaataa accgtggccc gccatgccac agagttttca
     3901 taatttatgt caacgtgcgg ctacggcggc gggctatcca gatttccagc cagatgcttg
     3961 tcttatcaac cgctacgctc ctggcgcgaa actgtcgctg catcaggata aagacgaacc
     4021 ggatctgcgc gcgccaattg tttctgtttc tctgggctta cccgcgattt ttcaatttgg
     4081 tggcctgaaa cgtaatgatc cgctcaaacg tttgttgttg gaacatggcg atgtggtggt
     4141 atggggcggt gaatcgcggc tgttttatca cggtattcaa ccgttgaaag cggggtttca
     4201 tccactcacc accgactgcc gctacaacct gacattccgt caggcaggta aaaaagaata
     4261 aaaataagaa ttattattgc tgtgcgcgcg aagatgttta aactgcgggc tgtaattcat
     4321 tgtccgggtt ttctgcatgg aacttcttgt acttgtctgg cggcagtatc gctggccatt
     4381 tatcagtgtg atggcgctaa gcctcgccag tgcggcatta ggcattggct taattgcttt
     4441 tatcaatcag cgccttatcg aaacggcgga taccagtctg ctggtgttgc cggagtttct
     4501 tggattatta ctgctgttga tggcagtcac tctcggatcg caactggcgc tcaccacttt
     4561 ggggcatcac ttcgtttacc gactgcgtag tgaatttatc aagcggattc tggataccca
     4621 cgtcgagcgc attgaacaac tcggtagcgc ctcgttgctg gcgggattaa ccagcgatgt
     4681 gcgcaatatc accattgctt ttgtgcgtct tccggaactg gtgcagggga tcattctcac
     4741 tatcggttcc gcggcgtatc tgtggatgct gtcgggcaaa atgttgctgg taacggctat
     4801 ctggatggcg atcaccatct ggggtggttt tgtactggtg gcgcgggtgt acaaacatat
     4861 ggcgaccctg cgtgaaaccg aagacaagct gtacacggat tttcaaaccg tactcgaagg
     4921 gcgcaaagag ctgactctga accgggaacg cgccgagtat gtgtttaaca acctctacat
     4981 ccccgatgcg caagagtatc gccatcatat tatccgcgca gacaccttcc atcttagcgc
     5041 cgtgaactgg tcaaacatca tgatgctggg cgctatcggc ctggtgttct ggatggcgaa
     5101 cagtctcggt tgggctgata ccaacgttgc tgcgacctat tcgttgacgc tgttattcct
     5161 gcgtacgccg ctgctttcgg cggttggcgc attgccgacg ctgctgacgg cgcaggtggc
     5221 gtttaacaag ctgaacaaat tcgcgctcgc gcctttcaaa gctgagtttc cgcgcccgca
     5281 ggcttttccc aactggcaaa cgctggagct gcgtaacgtg acgtttagct atcaggataa
     5341 cgcgttttcc gttgggccga ttaacctcac cattaaacgt ggcgagctgc tgtttctgat
     5401 tggtggcaac ggtagtggaa aatcgacgct ggcgatgttg ctgacgggct tgtaccagcc
     5461 acaaagcggc gaaatcttgc tggatggcaa acctgtcagt ggcgaacaac cggaagatta
     5521 tcgcaaactg ttttcggcag tgtttaccga tgtctggctg tttgatcaac tgctggggcc
     5581 ggagggtaaa cccgctaacc cgcaactggt tgagaagtgg ctggcgcagc tgaaaatggc
     5641 tcataagctt gagttaagca acgggcgtat tgttaacctg aagttatcaa aagggcagaa
     5701 aaaacgcgtg gcgctgttgc tggcgctggc agaagaacgc gatattatcc tgctggatga
     5761 atgggcggcg gatcaggatc cacacttccg tcgtgagttt tatcaggtgt tgctgccgct
     5821 gatgcaggag atgggtaaaa ctattttcgc tatcagtcat gatgatcatt actttatcca
     5881 cgccgaccgc ctgctggaaa tgcgcaatgg gcaacttagc gagctga
//
LOCUS       DAZFQD010000210         5781 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.210, whole genome shotgun sequence.
ACCESSION   DAZFQD010000210 DAZFQD010000000
VERSION     DAZFQD010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5781
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.210"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..3050)
                     /locus_tag="ACHKTW_004879"
     CDS             complement(<1..3050)
                     /locus_tag="ACHKTW_004879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
                     /protein_id="HFO6465751.1"
                     /translation="MSEGPGGPQGATAGGTLAMRMLSQQAMVASQMKRAANDKAIAQM
                     LAAKKSGPPAARLGDEIQHKSFLGALAGAVLGAIVTIAEGCLIMAACATGPYALVLVP
                     ALMYASYKASDYVEEKQNQLESWINSFCDTDGAINTGSENVNINGKPAARAAVTLPPP
                     PPPGAIPEVPQGEPSWGDIATDLLESAAEKAVPLAKAWGNAVITLTDSNAGFMDRVSA
                     GASLLFPAGPVLMEFATMVGGRGEIKKEVDFPEAGEDSALCDKENKPPRIAQGSSNVF
                     INNQPAARKGDKLECSAAIVEGSPDVFIGGEQVTYLDIQPEFPPWQRMILGGITIASY
                     LLPPAGLLGKLKNLARLGKLGNLLGKSGKLLGAKLGALLGKTGKSLKSIANKVIRWVT
                     DPVDPVSGAYCDERTDFTLGQTLPLSFTRFHSSVLPLHGLTGVGWSDSWSEYAWVREQ
                     GNRVDIISQGATLRFAFDGDSDTAVNPYHAQYILRRRDDYLELFDRDALSSRFFYDAF
                     PGMRLRHPVTDDTDDDRLAHSPADRIYMLGGMSDTASNRITFERDSQYRITGVSHTDG
                     IRLKLTYHASGYLKAIHRTDNGIQTLATYEQDARGRLTEADARLDYHLFYEYDAADRI
                     IRWSDNDQTWSRFTYDEQGRCVTVTGAEGYYNATLDYGDGCTTLTDGKGTHRYYYDPD
                     GNILREAAPDGSTTTYEWDEFHHLLARHSPAGRVEKFEYNAAHGQLSRYTAADSAVWQ
                     YRYDERGLLSNITDPAGQTWTQQCDERGLPVSLVSPQGEETRLAYTPQGLLSGIFRQD
                     ERRLGIEYDHHNRPETLTDVMGREHHTEYSGHDLPVKMRGTGGQSVRLQWQQHHKLSG
                     IERAGTGAEGFRYDRHGNLLAWTDGNGVVWTMEYGPFDLPVARTDGEGHRWQYRYDKD
                     TLQLTEVINPQGESYRYILDNCGRVTEERDWGGVVWRYRYDADGLCTARVNGLEETIL
                     YSRDAAGRLAEVITPEGKTQYAYDKSGRLTGIFSPDGTSQRTGY"
     gene            complement(3070..3531)
                     /locus_tag="ACHKTW_004880"
     CDS             complement(3070..3531)
                     /locus_tag="ACHKTW_004880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
                     /protein_id="HFO6465752.1"
                     /translation="MQFTFNEGHIQLPSQWQDQSMQVLVSTDNSGINLVITREAVPQG
                     TLTPELYQETLALYQGKLDGYTEHACREITLAEAPAWLLDYSWNGPEDEGNQGRISQI
                     AVFQRRGDTLLTFTFSTSLSLKNSQKTMLLEVIKSFTPLPPENDIQKDQPR"
     gene            complement(3559..5460)
                     /locus_tag="ACHKTW_004881"
     CDS             complement(3559..5460)
                     /locus_tag="ACHKTW_004881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HFO6465753.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTIWQGDEVQRRVKGVVTWFELGENDKNQMLYSMKVHPPLWRA
                     GLRQNFRIFQNEDIKSILGTMLQENGVTEWSPLFSEPHPSREFCVQYGETDYDFLCRM
                     AAEEGIFFYEEHAYKSTDQSLVLCDTVRHLPESFEIPWNPNTRTEVSTLCISQFRYSA
                     QIRPSSVVTKDYTFKRPGWAGRFEQEGQHQDYQRTQYEVYDYPGRFKSAHGQNFARWQ
                     MDGWRNNAETARGMSRSPEIWPGRRIVLTGHPQANLNREWQVVASELHGEQPQAVPGR
                     SGSGTTLNNHFAVIPADRTWRPQPLLKPLVDGPQSAVVTGAAGEEIFCDEHGRVRVKF
                     NWDRYNPADQDSSCWIRVAQAWAGTGFGHLAIPRVGQEVIVDFLNGDPDQPIIMGRTY
                     HQENRTPGSLPGTKTQMTIRSKTYMGSGFNELKFDDATGREQVYIHAQKNMDTEVLND
                     RTTTVKHDHRETVKNDQTVTIQEGNRLLTVEKGHKITGVLKGSLSEDVFQDRGTIAGS
                     VHVDAVNNGGEGDGIQAYTAIKEILLAVEESKIALTPDGIQLQVGESTVIRLSKDGIT
                     IVGGSVFIN"
BASE COUNT         1111 a         1708 c         1575 g         1387 t
ORIGIN      
        1 tcgtagccgg tgcgctgtga tgtgccgtcc gggctgaaga tacccgtcag cctgccggat
       61 ttgtcatagg catactgcgt tttgccttcc ggggtgatga cttctgccag gcggcctgcg
      121 gcgtcccggc tgtagaggat ggtttcctcc aggccgttga ccctggcggt acacaggcca
      181 tcggcgtcat agcggtaacg ccagaccacg ccgccccagt cacgctcttc cgtcacccgt
      241 ccacagttgt ccagaatata acggtatgac tcgccctgcg gattgatgac ttctgtcagt
      301 tgcagcgtgt ctttatcgta gcggtactgc cagcggtggc cttcaccgtc cgttcgcgcc
      361 accggcaaat cgaacgggcc gtattccatc gtccagacaa cgccgttgcc gtccgtccag
      421 gccagcaggt tgccgtggcg gtcatagcga aatccttccg caccggttcc agcacgttca
      481 atgccgctca gtttatggtg ctgctgccac tgtaaccgca ctgactgacc gccggtgccg
      541 cgcatcttca ccggcaggtc gtgaccactg tattcggtgt ggtgttcacg gcccatcacg
      601 tcggtgagtg tttccggccg gttgtggtgg tcgtactcta tgcccagacg ccgttcatcc
      661 tggcggaata tccccgacag cagcccctgc ggcgtgtacg ccagccgggt ctcttcgccc
      721 tgtggcgaca ccaggcttac cggcaggccg cgttcatcac actgctgtgt ccacgtctgc
      781 ccggctgggt cggtgatgtt gctgagcagg ccgcgctcat catagcggta ctgccacacc
      841 gcgctgtctg ccgccgtata acggcttaac tgaccgtgtg cggcgttgta ttcaaacttc
      901 tccacccgcc cggcagggga gtggcgggcc agcaggtgat ggaattcatc ccattcatac
      961 gtggtggtgc tgccgtccgg cgctgcttcc cgcagaatat tgccgtcagg atcatagtaa
     1021 taacggtgag tgcccttgcc gtccgtcagg gtggtgcagc cgtcaccata gtccagcgtg
     1081 gcgttgtaat agccctccgc cccggtgacg gtcacgcacc ggccctgttc atcgtaggtg
     1141 aaacggctcc acgtctggtc gttatcggac cagcggatga tccggtccgc agcgtcgtac
     1201 tcataaaaca ggtggtagtc cagccgcgca tccgcttcgg tcagccgccc ccgcgcatcc
     1261 tgttcgtagg tcgccagcgt ctgtatgccg ttatccgtgc ggtgaatggc tttcaggtag
     1321 ccgctggcat ggtacgtcag tttaagccgg atcccgtcgg tgtgactgac acccgtgatc
     1381 cggtactggc tgtcgcgctc aaacgtgatg cggttgctgg cggtgtcgct catcccgccc
     1441 agcatgtata tccggtctgc ggggctgtgt gccagacggt catcgtcggt atcgtcagtc
     1501 accgggtggc gcaggcgcat tcccggaaag gcgtcataaa agaagcggct gctcagggca
     1561 tccctgtcga acagctccag gtaatcatcg cggcggcgca gaatgtactg ggcgtgatac
     1621 gggttaaccg ccgtatcact gtcaccgtcg aaggcaaatc tcagcgtggc tccctggctg
     1681 atgatatcca cccggtttcc ctgttcacgc acccaggcgt attcgctcca ggagtcgctc
     1741 cagcccacgc ccgtcaggcc atgcagtggc agtacagaac tgtggaaacg ggtgaaggag
     1801 agggggaggg tctggccaag ggtgaagtcg gtacgttcgt cgcagtatgc gccgcttacc
     1861 ggatcgacag gatctgttac ccatctgatg actttattgg caatactttt taacgactta
     1921 cctgttttcc ccagcaacgc gccgagcttt gcgcccagca gcttcccgct tttccccagc
     1981 aggtttccca gtttgccgag tctcgccaga ttcttcagtt ttcccagcag tcccgctggc
     2041 ggcagaagat agctggctat cgttattcct cccaggatca ttctctgcca tggcgggaac
     2101 tccggctgga tatccagata ggtgacctgc tcacccccaa taaagacgtc cggcgaacct
     2161 tccacgattg ccgcgctgca ctccagtttg tcgcccttgc gcgcggcagg ctgattgttg
     2221 ataaagacgt tgctgctgcc ctgggctatc ctcggtggtt tgttctcctt gtcgcagagc
     2281 gccgagtcct caccggcttc cgggaaatcc acctcttttt tgatttcgcc acgcccgccc
     2341 accatggtgg caaactccat taataccgga ccggcgggaa acagaagcga tgcgccggcg
     2401 ctgacgcgat ccataaaacc ggcattgctg tccgtcaggg tgataacagc gttcccccag
     2461 gccttcgcca gtggtactgc tttttccgct gccgattcaa gcaggtcagt ggcaatatca
     2521 ccccatgagg gttccccctg tgggacttca ggtattgctc caggtggggg aggaggggga
     2581 agggtgacgg cggccctggc agcgggcttt ccgttaatgt ttacattttc agaaccggta
     2641 ttgatggcgc cgtccgtgtc acaaaagctg tttatccatg actcaagctg gttctgtttc
     2701 tcctccacat aatcactcgc cttatagctg gcatacatca gcgcaggcac cagaaccagc
     2761 gcataagggc cggtggcaca ggcggccata atcaggcaac cttctgcgat ggtcactatc
     2821 gcccccagca cggcccctgc cagtgccccc agaaaacttt tatgctgaat ttcatcgccc
     2881 agcctggcgg caggtgggcc ggacttcttt gctgccagca tctgtgcaat ggctttgtcg
     2941 ttggctgccc gtttcatctg gctggcgacc atcgcctgct gtgacagcat tcgcattgcc
     3001 agcgtaccgc ctgcggtcgc tccctgtggc ccgcctggtc cttcactcat gtgttatccc
     3061 tcgtttatgt cagcgcggct ggtccttttg gatgtcattc tccggtggca acggggtgaa
     3121 gctctttatc acctccagca gcatcgtctt ttgactgttc ttcagggata aggatgtgct
     3181 gaaagtgaag gtcagcagcg tatctccacg ccgctggaat acggctatct ggctaatccg
     3241 cccctgattc ccctcatctt ccggaccgtt ccaggaataa tccagcagcc aggccggggc
     3301 ctctgccaga gtgatttccc ggcaggcatg ttcggtgtac ccatcaagtt tgccctggta
     3361 cagcgccagc gtttcctggt acagctctgg tgtcagggtt ccctgtggaa cggcttcgcg
     3421 ggtaatgacc aggttgatgc cactgttatc tgtggacacc aggacctgca ttgactgatc
     3481 ctgccattga gacggcagtt gaatgtggcc ttcattaaaa gtgaactgca taaaatactc
     3541 cctgataatc tgagtgaatc agttgatgaa aacagaaccg cccacgatgg tgatgccatc
     3601 cttcgacagc ctgattaccg tcgattcccc gacctgtagc tgaatgccat ccggcgtcag
     3661 cgcaattttg ctttcctcca cagccagcaa aatttcctta atcgccgtat aagcctgtat
     3721 accgtcgcct tcgccaccat tgtttacagc gtcaacatgc accgaaccgg caatcgtgcc
     3781 tctgtcctga aagacatcct cagataaaga ccctttcagt actccggtga tcttgtggcc
     3841 tttttccacc gtaagaaggc ggttaccttc ctggatcgtg accgtctggt cattttttac
     3901 ggtttcgcgg tgatcgtgtt ttacggtggt ggtacggtcg ttgagcactt cggtatccat
     3961 gttcttctgc gcgtggatgt agacctgttc tctccctgtc gcatcatcaa acttcagctc
     4021 attaaatccg ctgcccatgt acgttttgga gcggatggtc atctgcgtct ttgttcccgg
     4081 caggctgccg ggggtgcggt tttcctggtg gtaggtgcgc cccataatga tcggctggtc
     4141 cggatcgccg ttgaggaagt ctacaatcac ctcctgaccc acacgcggta tcgccaggtg
     4201 gccaaaaccg gtgcctgccc acgcctgtgc cacacggatc cagcacgaac tgtcttggtc
     4261 tgccgggtta taacggtccc agttgaactt cacccgcacg cgaccatgtt cgtcgcagaa
     4321 gatttcctcg cctgccgctc ctgtcacgac agcgctctgc gggccgtcca ccagcggttt
     4381 cagcaacggc tgtggtcgcc atgttctgtc tgccgggatc accgcaaagt gattattcag
     4441 cgtggtgcct gaaccactgc gtcccggcac cgcctgtggc tgttcgccgt gcagttcact
     4501 tgccaccacc tgccattccc ggttcaggtt cgcctgcgga tgccccgtca gcacaattcg
     4561 ccgtcccggc catatctccg gcgagcggct cattccccgc gcggtttctg cgttgtttcg
     4621 ccagccgtcc atctgccagc gggcaaagtt ctgcccgtgg gcgctcttga aacgtccggg
     4681 gtagtcatac acttcatact gcgtgcgctg gtagtcctgg tgctggcctt cctgttcaaa
     4741 acgtccggcc cagccggggc gtttaaaggt gtagtctttg gtcaccacgg aagaagggcg
     4801 gatttgtgcg ctgtagcgga actggctgat gcagagggtg ctcacctcgg tacgggtgtt
     4861 cgggttccat gggatttcaa aagattcggg cagatggcgg actgtgtcgc acagcaccag
     4921 gctctggtcg gtacttttgt aagcatgctc ctcataaaag aagatgcctt cctccgccgc
     4981 catccggcac aggaaatcgt aatcagtctc accgtactgg acacaaaact cacgggaagg
     5041 atgcggctcg ctgaacagcg gactccattc ggtcaccccg ttttcctgca acatcgtgcc
     5101 gaggatgctt ttgatgtcct cgttctggaa gatacggaag ttctggcgca gaccggcacg
     5161 ccacagcggc gggtgcacct tcatgctgta cagcatctgg tttttgtcgt tctccccgag
     5221 ttcaaaccag gtcaccacgc ctttcacccg gcgctgtact tcatcgccct gccatatcgt
     5281 caggtaggcc attttgtcca gcacctgcgc aaattcaagg gagagaaact gctggctgac
     5341 cagggagaga tcgagggaaa agagcgaaga gagtgactgg ttcagatgaa aggaaaccac
     5401 cgcaaaagca tccggcggca ggccgtccac ttccagtgtg aaacgtaatc cggttgacat
     5461 agcaacctcc agtaaaatga ataatgatcc gtaccgtaca actgcggata atgtgataag
     5521 cgggacgact tcagacgtta ttagggaagg aacagaaaat gctatggtta tgtgagaatt
     5581 ttgtttaaat attactttac agaaacgggt gtctgttcag tcaaaaaaat aatcacaagc
     5641 tcaacctctt gtttcttggc gttattcagg acaggtacgc atatcacaac gagtaacaaa
     5701 aacgtaaagt taaaccaata ttcataatat atttttattc acagcaataa ataatccaat
     5761 taacattatt aaaatgaatg a
//
LOCUS       DAZFQD010000211         5761 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.211, whole genome shotgun sequence.
ACCESSION   DAZFQD010000211 DAZFQD010000000
VERSION     DAZFQD010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5761
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.211"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            6..995
                     /gene="ldhA"
                     /locus_tag="ACHKTW_004882"
     CDS             6..995
                     /gene="ldhA"
                     /locus_tag="ACHKTW_004882"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFO6465754.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSRPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            1106..1528
                     /gene="hslJ"
                     /locus_tag="ACHKTW_004883"
     CDS             1106..1528
                     /gene="hslJ"
                     /locus_tag="ACHKTW_004883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HFO6465755.1"
                     /translation="MKKVAALVALSLLMAGCVSNDKIAVTPEQLQHHRFVLESVNGKP
                     VTNDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     NTISEMLKEGAQVDLTANQLTLATAKQTLTYKLADLMN"
     gene            complement(1525..1791)
                     /gene="ydbJ"
                     /locus_tag="ACHKTW_004884"
     CDS             complement(1525..1791)
                     /gene="ydbJ"
                     /locus_tag="ACHKTW_004884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HFO6465756.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPGLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            2065..5589
                     /gene="nifJ"
                     /locus_tag="ACHKTW_004885"
     CDS             2065..5589
                     /gene="nifJ"
                     /locus_tag="ACHKTW_004885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HFO6465757.1"
                     /translation="MITIDGNGAVASVAFRTSEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYDAVYDHVEQAMNDFSAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQALPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGERETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTLPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPGG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRLEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACSGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAADLQKRYDFLVQMAGKAEKSNTD"
BASE COUNT         1446 a         1481 c         1486 g         1348 t
ORIGIN      
        1 gtcttatgaa actcgccgtt tatagcacaa aacagtacga caagaagtac ctgcaacagg
       61 tgaacgagtc ctttggcttt gagctggaat tttttgactt tctgctgacg gaaaaaaccg
      121 ctaaaactgc caatggctgc gaagcggtat gtattttcgt aaacgatgac ggcagccgcc
      181 cggtgctgga agagctgaaa aagcacggcg ttaaatatat cgccctgcgc tgtgccggtt
      241 tcaataacgt cgaccttgac gcggcaaaag aactggggct gaaagtagtc cgtgttccag
      301 cctatgatcc agaggccgtt gctgaacacg ccatcggtat gatgatgacg ctgaaccgcc
      361 gtattcaccg cgcgtatcag cgtacccgtg acgctaactt ctctctggaa ggtctgaccg
      421 gctttactat gtatggcaaa acggcaggcg ttatcggtac cggtaaaatc ggtgtggcga
      481 tgctgcgcat tctgaaaggt tttggtatgc gtctgctggc gttcgatccg tatccaagtg
      541 cagcggcgct ggaactcggt gtggagtatg tcgatctgcc aaccctgttc tctgaatcag
      601 acgttatctc tctgcactgc ccgctgacac cggaaaacta ccatctgttg aacgaagccg
      661 ccttcgatca gatgaaaaat ggcgtgatga tcgtcaatac cagtcgcggt gcattgattg
      721 attctcaggc agcaattgaa gcgctgaaaa atcagaaaat tggttcgttg ggtatggacg
      781 tgtatgagaa cgaacgcgat ctattctttg aagataaatc caacgacgta attcaggatg
      841 acgtattccg tcgcctgtct gcctgccaca acgtgctatt taccgggcac caggcattcc
      901 tgacagcaga agctctgacc agtatttctc agactacgct gcaaaactta agcaatctgg
      961 aaaaaggcga aacctgcccg aacgaactgg tttaatcttg ccgctcccct gcattccagg
     1021 ggagctgatt cagataatcc ccaatgacct ttcatcctct attcttaaaa tagccctgag
     1081 tcagaaactg taattgagaa ccacaatgaa gaaagtagcc gcgctcgttg cgctaagcct
     1141 gctgatggcg ggatgtgtaa gtaatgacaa aattgctgta acgccagaac agttacagca
     1201 tcatcgtttt gtgctggaaa gcgtaaacgg taagcccgtg accaacgata aaaatccgcc
     1261 agaaatcagc tttggtgaaa aaatgatgat ttccggcagc atgtgtaacc gctttagcgg
     1321 tgaaggcaaa ctgtctaatg gtgaactgac agccaaaggg ctggcaatga cccgtatgat
     1381 gtgcgctaac ccgcagctta atgaactcga taacaccatt agcgaaatgc tgaaagaagg
     1441 tgcacaagtg gatctgaccg cgaaccagtt aacgctggcg accgcgaaac agacattaac
     1501 ttataagctg gcggatttaa tgaattaata gctgccacag ctcccggcgg caagtgactg
     1561 ttcactacag cgtttgccgt tgggtaatgc acacatccca atcgccgtac catccagttg
     1621 acgggcaaca gaaagcgaac cgccgatcat tgcacaattt gcttctccac tactggacat
     1681 cgacgctttt aaacctggcg ctacgtgcgc cgcagtcgcc tgctgaacag gttcactact
     1741 acacgccgac aacaataaag cggcacaccc tacccaaaac gctgctcgca tcttttcttc
     1801 ctctgatctt caagccaaac gacaccgcca taaataatag gcagcacaga gggcgacgtc
     1861 gagagctgtc ctgcgcgttg ccccgcattt ttactttttt atggctattt ttttgccctc
     1921 tgtttgatca aaacattcat tacgctgatg tgggggacac aaaagcgaaa atgcagaaga
     1981 aagccatttg ctaaaattga aggattacta ctgggcgcgc agcaatttcg tgcgcccctc
     2041 atttgcgcaa tgtaagggtg tcatatgatt actattgacg gtaatggcgc ggttgcttcg
     2101 gtggcgtttc gcaccagtga agttatcgcc atctacccta ttacccccag ttccacgatg
     2161 gcagaacagg ctgatgcctg ggccggaaac ggcttaaaga acgtttgggg agacacacca
     2221 cgcgtagttg aaatgcagtc ggaagcgggt gctatcgcta ccgtgcatgg cgctttgcag
     2281 acgggtgccc tttcaacatc gtttacgtca tcgcagggtt tgctgctgat gatcccgacg
     2341 ctgtacaaac tggcaggcga actgacgcca tttgtcctgc atgtagcggc acgtaccgtt
     2401 gccacacatg cactttctat ttttggcgat cattccgacg ttatggcggt gcgccagacg
     2461 ggttgcgcga tgttgtgtgc ggctaacgtc caggaagcgc aagattttgc tctcatttcg
     2521 cacatcgcaa cgctgaagag ccgcgtgcca tttattcatt tctttgatgg tttccgcacg
     2581 tcccacgaaa tcaataaaat tgtcccgctg gccgatgaca ctattcttga tctcatgccg
     2641 caggccgaaa ttgatgctca tcgcgcccgg gcgctcaacc cggaacatcc ggtgatccgc
     2701 ggtacgtccg ccaatcctga cacttatttc cagtctcgcg aagccaccaa cccatggtac
     2761 gacgcggtct atgaccatgt tgaacaggcg atgaatgatt tctctgccgc gacaggtcgt
     2821 cagtatcagc cgtttgaata ttacgggcat ccgcaagcgg aacgggtgat tatcctgatg
     2881 ggctctgcca ttggcacctg tgaagaagtg gttgatgaat tgctaacccg tggcgaaaaa
     2941 gttggcgtgc tgaaagttcg cctgtaccgt cccttctccg ccaaacactt gctgcaagct
     3001 ctgccgggat ccgtacgcag cgtggcggta ctggacagaa ccaaagaacc cggtgcccag
     3061 gcagaaccgc tctatctgga tgtaatgacc gcactggcag aagcctttaa taatggcgag
     3121 cgcgaaactc tgccccgtgt cattggtggg cgctatggtc tttcatccaa agaatttggc
     3181 ccggactgtg tactggcggt atttgccgag ctcaacgcgg ctaaaccgaa agcgcgcttt
     3241 acggttggta tttacgatga tgtgaccaat ctgtcactgc cgttgccgga aaacaccctg
     3301 ccaaactcgg cgaaactgga agccttgttt tatggccttg gtagtgatgg cagcgtttcc
     3361 gcgaccaaaa acaatatcaa gattatcggt aattccacgc cgtggtacgc acagggctat
     3421 tttgtttacg actccaaaaa ggcgggcggc ctgacggttt ctcaccttcg cgtgagcgaa
     3481 cagccgattc gttccgctta tctcatttcc caggctgatt ttgttggctg ccaccagttg
     3541 cagtttatcg ataaatatca gatggctgag cgtttaaaac ctggcggcat tttcctgctc
     3601 aacacgccgt acagcgcaga tgaagtgtgg tcgcgcttgc cgcaggaagt tcaggccgtg
     3661 ctaaatcaga aaaaagcgcg cttctatgtg attaacgcgg cgaaaatcgc ccgcgaatgt
     3721 ggcctggcgg cccgtattaa taccgtcatg cagatggctt ttttccatct gacgcaaatt
     3781 ctgcctggcg atagcgccct cgcagaattg cagggtgcga ttgccaaaag ttacagtagc
     3841 aaaggccagg atctggtgga acgcaactgg caggctctgg cgctggcgcg tgaatccgta
     3901 gaagaagttc cgttgcaacc ggtaaatccg cacagcgcca atcgaccgcc agtggtttcc
     3961 gatgccgccc ctgatttcgt gaaaaccgta accgcagcga tgctcgcagg gcttggtgat
     4021 gccctccccg tttcggcgct gccgccagac ggcacctggc cgatgggcac cactcgctgg
     4081 gaaaaacgca atatcgccga agagatcccc atctggaaag aggaactctg tacccaatgt
     4141 aaccactgcg tcgccgcttg cccacactca gctattcgcg caaaagtggt accgcctgaa
     4201 gcgatggaaa acgcccctgc cagcctgcat tcgctggatg tgaaatcgcg tgatatgcgc
     4261 gggcagaaat atgtcttgca ggtggctccg gaagattgca ccggttgtaa cctgtgcgtc
     4321 gaagtttgcc cggcgaaaga ccgtcagaat ccagagatta aagccatcaa tatgatgtcg
     4381 cgcctggaac atgtcgaaga agagaaaatc aattacgatt tcttcctcaa cctgccagaa
     4441 atcgaccgta gcaaactgga acgtattgat attcgtacat cgcagctgat tacaccgctg
     4501 tttgaatatt caggtgcttg ctccggttgt ggcgagacgc cgtatattaa attactgact
     4561 cagctctatg gcgaccggat gttgatcgct aacgccaccg gctgttcttc aatttatggc
     4621 ggtaacctgc cctctacacc gtataccacc gatgccaacg gtcgtgggcc ggcatgggcg
     4681 aactctctat ttgaagataa tgccgaattt ggccttggtt tccgcctgac ggtcgatcaa
     4741 caccgtgtcc gcgtgctgcg tctgctggat caatttgccg ataaaatccc ggcggaatta
     4801 ctgacagcgt tgaaatcaga cgctacgcca gaggttcgtc gagaacaggt cgcagcttta
     4861 cgccagcaac tcaacgatgt tgccgaagca catgaactgc tgcgtgacgc agatgcactg
     4921 gtggaaaaat caatctggct gattggtggc gacggctggg cttacgatat cggctttggc
     4981 ggtctggatc atgtattgag tttgacggaa aacgtcaaca ttctggtgct ggatacgcaa
     5041 tgctattcca acaccggtgg tcaggcgtcg aaagcgacac cgctgggtgc agtaactaag
     5101 ttcggcgagc acggcaaacg taaagcgcgt aaagatcttg gcgtcagtat gatgatgtac
     5161 ggtcatgttt atgtggcgca gatttctctc ggcgcgcagc tgaaccagac ggtgaaagcg
     5221 attcaggaag cggaagcgta tccggggcca tcgctgatca ttgcttatag cccgtgtgaa
     5281 gagcatggtt acgatctggc actcagccat gaccagatgc gccaactcac agctaccggc
     5341 ttctggccgc tatatcgctt cgatcctcgt cgtgccgatg aaggcaaact gccgctggcg
     5401 ctggattcac gcccgccgtc agaagcactg gaagaaacgt tacttcacga gcaacgtttc
     5461 cgtcgtctga attcgcagca gccagaagtg gcagaacagt tatggaaaga tgctgcagct
     5521 gatttgcaaa aacgctatga cttcctggta caaatggccg gaaaagcgga aaaaagcaac
     5581 accgattaaa tgctctggat aaggattatc caattctaaa aaaaaagccc ggacgactgt
     5641 tcgggcttgt ctttttatat gttggaaaat cagtggcaat gcaatggccc aacagaaaat
     5701 aaaatctgtg acaataaagg catataaccc gcgcagaata acgtatagca aattaaaagt
     5761 a
//
LOCUS       DAZFQD010000212         5738 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.212, whole genome shotgun sequence.
ACCESSION   DAZFQD010000212 DAZFQD010000000
VERSION     DAZFQD010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5738
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.212"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(291..1250)
                     /gene="nrdF"
                     /locus_tag="ACHKTW_004886"
     CDS             complement(291..1250)
                     /gene="nrdF"
                     /locus_tag="ACHKTW_004886"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFO6465758.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNANKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            complement(1260..3404)
                     /gene="nrdE"
                     /locus_tag="ACHKTW_004887"
     CDS             complement(1260..3404)
                     /gene="nrdE"
                     /locus_tag="ACHKTW_004887"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFO6465759.1"
                     /translation="MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     IATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDATLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADIAISEHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPIAGRINMSNLCSEIL
                     QVNSASEYDENLDYTYTGHDISCNLGSLNIAHTMDSPDFARTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYAITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFTRSGITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            complement(3377..3787)
                     /gene="nrdI"
                     /locus_tag="ACHKTW_004888"
     CDS             complement(3377..3787)
                     /gene="nrdI"
                     /locus_tag="ACHKTW_004888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFO6465760.1"
                     /translation="MSQLVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIARKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            complement(3784..4029)
                     /gene="nrdH"
                     /locus_tag="ACHKTW_004889"
     CDS             complement(3784..4029)
                     /gene="nrdH"
                     /locus_tag="ACHKTW_004889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HFO6465761.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            complement(4277..4606)
                     /locus_tag="ACHKTW_004890"
     CDS             complement(4277..4606)
                     /locus_tag="ACHKTW_004890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HFO6465762.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVGCADSFVRERPWCSVGTAAAVGIFIGA
                     LLSMRKS"
     gene            4758..5102
                     /locus_tag="ACHKTW_004891"
     CDS             4758..5102
                     /locus_tag="ACHKTW_004891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2002 family protein"
                     /protein_id="HFO6465763.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            complement(5139..5588)
                     /gene="alaE"
                     /locus_tag="ACHKTW_004892"
     CDS             complement(5139..5588)
                     /gene="alaE"
                     /locus_tag="ACHKTW_004892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HFO6465764.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
BASE COUNT         1461 a         1411 c         1435 g         1431 t
ORIGIN      
        1 tctaccctaa catactgaga atctgaggca acccctgatg gcaaatgtgg catgaatatt
       61 gacgcgagta aatcgccgga atttcccgtg atataagggc tgagagcaaa tcgagaaaag
      121 ttgatatttt ttgtcaaaga tagcgaaaaa acgcctgaat attgtggttt caggcgaata
      181 atgcaacaga gtatcagtgt agatcactac agaatatttg cgtgataaaa agctatttgg
      241 cgggaattat ttccctgctg cgggtagtga tatttttgaa aataacaccc tcagaaattc
      301 cagtcttcat cttctgtttc aaccgctttc cccatcacat aagaggagcc tgaaccggaa
      361 aagaaatcgt gattttcatc ggcattcggc gaaagcgcgg cgaggattgc cggattcact
      421 tccgccattt ctgcgggaaa taacggttcg tagcccagat tcatcaaagc cttattggcg
      481 ttgtaacaga gaaacgcttt cacatcgtca gcccacgggg tttcggcgta cagctcatcg
      541 gtgtattgca actcgttgtc gtagagttcc agcaacaaat cgaaggcgaa actcttcaac
      601 tcttcacgtt gtcccagaga tatcttttcc atgtttttct gatatttata gcctatgtag
      661 taaccgtgga ctgcttcatc gcggataatc agacggatca gatctgcggt attggtcagc
      721 tttccgcggc tggaaaaata catcggcagc cagaaaccgg aatagaacaa aaaagattca
      781 agaaacacac tggcgatttt ctttttcagc ggatcatcac cgcgataatg ttgctgaata
      841 atctgagctt ttcgctgcaa cggtgcgttt tcttcactcc aggcgtaggc ggcatcgaca
      901 tctttggtct gacacagcgt cgagaaaatc gaactgtaag agcgggcatg aaccgcttcc
      961 ataaagctga tattcgataa taccgcttct tcatgaggcg tgagtgcatc gggcatcagc
     1021 gaaggcgcgc cgataacatt ttgcagcgtg tcgagcagcg tcaggccagt aaaaacgcgc
     1081 atcgtcagtt gttgttctac gacagttaat gtctgccagg caggaatatc gttcgacagc
     1141 ggcacctttt ctggtagcca gaaattgctg gtcaggcgat tccacacctc cagatcttta
     1201 tcgtcagata tcttgttcca gttgatggcg ctgatacgtg agagtttcat agatattcct
     1261 taaagtgcac aggagacgca gccttcaatt tcagtgcctt ccagcgccat ctgacgcagg
     1321 cggatgtaat agagcgtttt gatacctttg cgccaggcgt aaatctgcgc tttgttgata
     1381 tcgcgagtgg tggcggtatc ggggaaaaac agcgtcagcg acagcccctg atcgacatgg
     1441 cgagtcgctt ccgcgtaggt gtcgatgatc ttttctgcgc cgatttcgta agcgtcctga
     1501 tatagcgcca gattttcgtt agtcataaac ggggcagggt agtaaacgcg tcctgttttg
     1561 ccctctttgc gtatctctac tttcgccaca atcggatgaa tactcgacgt agcatggttg
     1621 atataagaga tagaaccggt tggcggcacc gcctgaagat tctggttgta aatgccgtag
     1681 cgcatcacgt cgtcgcgcag ctgcgcccac atctcacggg taggtaacgt aataccgcta
     1741 cgggtaaaca gttcgccaac tttcgccgtt ttcggctgcc agttcccttg cagatattgg
     1801 ctaaaatatt caccactggc atagcgtgac tgtttgaacc cggcgaaggt ttcaccgcgt
     1861 tcgcgtgcca gcaacatcga ggtacgcagt gcgtgccagg tgatggcata gaaatagaga
     1921 ttggtgaaat ccagtgcttc cggcgaacca taagcgatgc cttctcgcgc cagatagccg
     1981 tgtaaattca tctgccccag tccgatggcg tgcgaggcgg catttccggc ttcgatggac
     2041 ggcacgctgc ggatatgact catatctgat actgccgtta aaccgcgcac ggcagtctct
     2101 accgtgcggg caaaatcggg ggaatccatg gtgtgcgcaa tattcaacga acctaaattg
     2161 caggagatat catggcctgt gtaggtatag tcgagattct cgtcatactc tgaggcgctg
     2221 ttaacctgca aaatttctga gcagagatta ctcatattta tgcgcccggc gatagggtta
     2281 gcacggttta ccgtgtcttc atacatgatg tagggatagc cggactcaaa ctggatttct
     2341 gccagtcgct ggaagaaatc acgggcgttg aggtattttt tgcggatgcg ttcatcggca
     2401 accagttcgt catagtgttc gctaatggcg atatcggcaa acggcttgcc ataaactcgc
     2461 tcaacgtcat agggcgaaaa cagcgccatc tgcgcattct cttttgccag atggaaagtg
     2521 atatccggga tcaccacgcc aagcgacagt gttttaatgc ggattttttc gtcggcattt
     2581 tcccgtttcg tgtcgagaaa acgcagaata tcgggatgat gagcatgtaa atagactgca
     2641 ccagccccct gacgcgcgcc gagttggttg gcataggaaa atgcgtcttc cagcattttc
     2701 atcaccggaa ttacgccaga agattgattt tcaatacgtt taattggcgc gcccgcttcc
     2761 cgcagattcg acagcaaaaa tgccacgccg ccgccgcgtt tcgacagttg cagtgcggaa
     2821 tttaccgccc ggccaatcga ctccatattg tcttcaatac gcagcagaaa acaggaaacc
     2881 agttcgccgc gctgctgctt accgcagttg aggaatgttg gcgtggctgg ctgaaagcgt
     2941 cctgacagca tttcatcggt cagttgcaac gccagcgtcg catcgccttg tgccagcgtc
     3001 agcgccacca tcgtgactcg atcggcaaaa tcttccagat aacgtttacc gtcgaatgtc
     3061 ttcaacgtat agctggtgta aaacttccat gccccgagga atgtctggaa acgaaaaccg
     3121 ctggtgtgtg cgtgggcaaa cagcgtaatg acaaagtcgc gagagtagcg attaagaacg
     3181 ctttcatcat agtaaccttc gttgaccagc cagttcaggc gctgctgctg gctactgaag
     3241 gtcacactgt tcggacgcac atgcgtcgca ataaaggcgt caacggcctg gcggtcttta
     3301 tcgaactgaa tgcgacctgc gctatcgtag aggttaagca tcgcattcag cgcgtggtaa
     3361 tccatcgttt cctgcgtcag gcattctgcg gttgtcgttg ccaaaattcg gttactcctt
     3421 tacgaacgtt ttcgatatcg ctttgcgtac ccatgagttc aaaacggtac agccacggca
     3481 cgccgcattt ccgggcaatc acatctccgg cgcggccata cgcctcacca aagttgcgat
     3541 taccagaagc aataacgccg cgaagcaacg cccggttgtg ctcgtcgttt aaaaagcgaa
     3601 ttacctgtcg tggcaccgcg ccagccgtac cgccgccgcc gtaagagggc acgatcagga
     3661 tgtaaggctc gtctacctga atccgttccc gctcattgag cgggatgcgc accgcgggca
     3721 gacctaaacg ttcgataaaa cgctgcgtgt tttcggagct gctggagaag tagacgagct
     3781 ggctcatgca ctggccgcgt gtggcgctgg atgcagacgg ttaatcatgt ccggacggaa
     3841 accagaccag ctaagatcgc cagcaatcac taccggcaac tgacgaaagc cctgagcacg
     3901 caacgcttcc gctgcttcag gaacgcgatc gacattaatc atttcaaaat caaagccccg
     3961 gttttccatc gcccgtttgg tggcgtggca ctgaacgcaa tcgttacgag tgtaaatagt
     4021 aatgcgcatg attcgtattt ccgtttaaaa tgaagatacg gcgcgatgat acgcgtcggg
     4081 ttgtctctct gttgatacag agatactaga tgtagttgaa aaaagattca accacacaat
     4141 atatagcaaa tggtggtcga aattaccctg gatatgagcg tgacggggta gggggatttt
     4201 tgtgattcac caggcaaaaa gaaaccccga agccaggctt cggggtcaaa gacgcgtatt
     4261 tattatcatt tttgcactac gatttgcgca tgcttaacag tgcgccgata aaaataccta
     4321 ccgcagctgc tgtacccacg ctgcaccagg gtctttcacg aacaaaagaa tccgcgcagc
     4381 caacggcatc gcgtgcggct tgctggacgc gagtacgacc atgcattcgc gccctggttt
     4441 ctttcagcaa tgcctgagct ttactgcgtg cggcttcagc ttcccctttg gcgtcgctgc
     4501 cccaggactt caatacagat tcgaggctgt cagctaattg attgacatca ttctggatat
     4561 cctgcacgcc atcatcaacg tcgttgcggt tcggtcggtt aaacatatga tcccccatta
     4621 gaattcgatg taattttcag tgtagaccat aattttctta actgaggttc atcgcggctt
     4681 tatggacttt tctgaaagcg ttgtgaatgc gcaatcaggt aaggtaggcc cgcggttgat
     4741 aataacgagg atcaaatatg tatttacgac cagacgaggt ggcgcgcgta cttgaaaaag
     4801 tcggttttac tgtcgatgtg gtaacccaga aagcgtatgg ttaccgccgt ggggaaaatt
     4861 atgtctatgt taatcgcgaa gcgcgaatgg gacgtaccgc tctggtaatt catccaacat
     4921 taaaagaacg cagttcaaca ctggctgaac ccgcttccga tattaaaact tgcgatcatt
     4981 accagcaatt tccgctctat ttagcaggcg agcgacacga gcattacggt atcccgcatg
     5041 gctttagttc gcgtgttgcg cttgaacgtt atttgaatgg tttatttggc gaagccagtt
     5101 aaagacgcga ctggcgatgc cagtcgcgaa aagaagagtc aggcttttac ctgctggtaa
     5161 cggctgactt taaacagtcg gcggcaataa tcgaggaagt agccataaac cgcccccatc
     5221 aacatcgaaa caacgatgtt tgaactgacc gccgccataa tctgatgcca gtctgcgccc
     5281 accactaaca agatcgccac atacaccggt gactggaacg tcacataagc caggatatca
     5341 gccagatttt ttatccagcc cgacgggcta actttgcgtg ccgcgcgcat aaacagatcg
     5401 cggtacatac cgtatggcca tgcaattaag atgttcaccg gaatcgctac caatctggaa
     5461 taaaaagact gttcgaagct cattccggag aggaaaactt caatacacat gttcacgaca
     5521 gaacagtaaa caaccatcgc gaacgtatct gcaactgcat gacgcaagcg tgactgcggt
     5581 gagaacatgc taatgctcct taataaaagc gtaatgggat gttaatggag atgaaagcag
     5641 tggatctcat tgccatctgc attttcgcat agcgtatatc gctggctatg actattcaat
     5701 tagtgaaaaa tttttattta gtcagttttt gtagataa
//
LOCUS       DAZFQD010000213         5597 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.213, whole genome shotgun sequence.
ACCESSION   DAZFQD010000213 DAZFQD010000000
VERSION     DAZFQD010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5597
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.213"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            162..1496
                     /gene="adeQ"
                     /locus_tag="ACHKTW_004893"
                     /gene_synonym="yicO"
     CDS             162..1496
                     /gene="adeQ"
                     /locus_tag="ACHKTW_004893"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HFO6465765.1"
                     /translation="MNNDNTDYVSNESGTLSRLFKLPQHGTTVRTELIAGMTTFLTMV
                     YIVFVNPQILGAAQMDPKVVFVTTCLIAGIGSIAMGIFANLPVALAPAMGLNAFFAFV
                     VVGAMGISWQTGMGAIFWGAVGLFLLTLFRIRYWMISNIPLSLRIGITSGIGLFIALM
                     GLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFIITVLSSRHFHAAVLVSIVVTSCC
                     GLFFGDVHFSGVYSIPPDISGVIGEVDLSGALTLELAGIIFSFMLINLFDSSGTLIGV
                     TDKAGLIDSNGKFPNMNKALYVDSVSSVAGAFIGTSSVTAYIESTSGVAVGGRTGLTA
                     VVVGVMFLLVMFFSPLVAMVPPYATAGALIFVGVLMTSSLARVNWDDFTESVPAFITT
                     VMMPFTFSITEGIALGFMSYCIMKVCTGRWRDLNLCVVVVAALFALKIILVD"
     gene            1549..2001
                     /locus_tag="ACHKTW_004894"
     CDS             1549..2001
                     /locus_tag="ACHKTW_004894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HFO6465766.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDGSEQNLLQWHRILQKAR
                     LAAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            2212..3402
                     /gene="nepI"
                     /locus_tag="ACHKTW_004895"
     CDS             2212..3402
                     /gene="nepI"
                     /locus_tag="ACHKTW_004895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015211 - purine nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015860 - purine nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HFO6465767.1"
                     /translation="MSEFIAENRGADAITRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGSFLGELIGWRNVFNAAAVMGVLCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMNLAGFGVDGLTLVLLSFGIASFIGTSLSSFIL
                     KRSVKLALAGAPLILAVSALVLTLWGSDKIVATGVAIIWGLTFALVPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVK
                     MKKS"
     gene            complement(3443..3736)
                     /gene="yicS"
                     /locus_tag="ACHKTW_004896"
     CDS             complement(3443..3736)
                     /gene="yicS"
                     /locus_tag="ACHKTW_004896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YicS"
                     /protein_id="HFO6465768.1"
                     /translation="MKPTTLLLIFTFFAMLGIVYAESPFSSLQSAKEKTTVLQDLRKI
                     CTPQASLSDEAWEKLMLSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
     gene            3958..4776
                     /gene="nlpA"
                     /locus_tag="ACHKTW_004897"
     CDS             3958..4776
                     /gene="nlpA"
                     /locus_tag="ACHKTW_004897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpA"
                     /protein_id="HFO6465769.1"
                     /translation="MKLTTHHLRTGAALLLAGILLAGCDQSSSDAKHIKVGVINGAEQ
                     DVAEVAKKVAKEKYGLDVELVGFSGSLLPNDATNHGELDANVFQHRPFLEQDNQAHGY
                     KLVAVGNTFVFPMAGYSKKIKTVAQIKEGATVAIPNDPTNLGRALLLLQKEKLITLKE
                     GKGLLPTALDITDNPRHLQIMELEGAQLPRVLDDPKVDVAIISTTYIQQTGLSPVHDS
                     VFIEDKNSPYVNILVAREDNKNAENVKEFLQSYQSPEVAKAAETIFNGGAVPGW"
     gene            complement(4780..>5597)
                     /locus_tag="ACHKTW_004898"
     CDS             complement(4780..>5597)
                     /locus_tag="ACHKTW_004898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="carboxylate/amino acid/amine transporter"
                     /protein_id="HFO6465770.1"
                     /translation="QFLTMTRLIFAGLILLTLSFVHGDKIFSIINNHKDAISLLIFSV
                     VGALTVQLTFLLTIEKSNAATATVLQFLSPTIIVAWFSLVRKSRPGILVFCAILTSLV
                     GTFLLVTHGNPTSLSISPAALFWGIASAFAAAFYTTYPSTLIARYGTLPVVGWSMLIG
                     GLILLPFYARQGTNFVVNGSLILAFFYLVVIGTSLTFSLYLKGAQLIGGPKASILSCA
                     EPLSSALLSLLLLGITFTLPDWLGTLLILSSVILISMDSRRRARKINRPARHE"
BASE COUNT         1335 a         1244 c         1447 g         1571 t
ORIGIN      
        1 tgattttatc aatttgatta ttatttatca taaagaaacg tcgcgagatt attaaagaat
       61 atccattaat gtgcaattga aatgtgataa ttatcactga accgttgttg tcatttgttt
      121 tacaaaagca agggattaaa tctcctccaa tggacaaaaa aatgaataat gacaataccg
      181 attacgtgag taatgaatca gggacgcttt cgcgattatt taaactacct cagcatggga
      241 ccaccgtccg cacagaattg attgcgggga tgaccacttt tttaaccatg gtgtacatcg
      301 tttttgtgaa cccgcaaatc ctcggcgcgg cacaaatgga cccgaaagtg gtgtttgtta
      361 ccacctgttt gattgccggt atcggcagta ttgcgatggg gatatttgct aacttacccg
      421 tggcgctggc tccggcaatg gggctgaacg ccttctttgc cttcgtggtc gtgggggcga
      481 tgggcatctc ctggcagacc gggatgggcg caatattctg gggcgcagtt ggactatttt
      541 tgctcacgct gtttcgtatc cggtactgga tgatctccaa cattccctta agtttacgta
      601 ttggtatcac cagcggaatt ggattattta tcgccttaat gggattaaaa aatactggcg
      661 ttattgtcgc caataaagac acgctggtga tgattggcga tttaagttct cacggcgtgt
      721 tgttaggtat tttagggttt tttattataa ccgtgttgtc atcacgtcat tttcatgccg
      781 cggtgctggt ttctattgtg gtgacgtctt gctgtggatt atttttcggt gatgttcatt
      841 ttagcggcgt ctattccatt ccgcctgata ttagcggcgt cattggtgaa gtagatttga
      901 gcggcgcgtt aacacttgaa ctcgccggta tcattttctc ctttatgctg atcaacctat
      961 ttgattcatc aggaacatta attggtgtaa ctgataaagc gggcttaata gatagtaacg
     1021 gtaaattccc caatatgaat aaggcgctgt atgttgatag cgtcagttcg gtggcggggg
     1081 cgtttatcgg cacctcgtct gttactgcct atattgaaag tacttctggt gtggcagtcg
     1141 gtggtcgcac ggggctgact gcggttgtgg ttggcgttat gttcctgtta gttatgttct
     1201 tctcaccgct ggtggcgatg gttcctcctt acgcaaccgc cggagcgtta atctttgttg
     1261 gcgtgctgat gacttcgagc ctggcgcgcg ttaactggga tgattttacc gaatcggtgc
     1321 ctgcgtttat taccacggtg atgatgccct ttactttctc gatcaccgaa gggattgcac
     1381 tcggctttat gtcgtactgc atcatgaaag tatgcaccgg gcgctggcgc gatctgaacc
     1441 tgtgtgtggt ggtggtcgca gctctgtttg cactgaagat tattctggtg gattagtaac
     1501 tggcgagggt ttccagatat catgagttct gattacgcag gagaactcat gatctggata
     1561 atgctcgcca cgctggcggt agtgtttgtg gttggttttc gggtgctgac atccggggcc
     1621 agaaaagcga ttcgccgtct cagcgatcgg ctgaacatcg atgtcgtacc cgtggagtcg
     1681 atggtcgatc aaatgggaaa gtcagctggt gacgaatttt tacgttattt gcatcgtccg
     1741 gatgagtcgc acctgcaaaa cgccgcgcag gtgttgctca tctggcaaat tgtcattgtc
     1801 gatggtagcg aacagaacct gctgcaatgg catcggattt tacaaaaagc tcgcctcgcc
     1861 gcgccgatta ccgacgctca ggtcaggctg gcgctaggtt ttctgcgcga aaccgaacct
     1921 gaaatgcagg atattaatgc ttttcagatg cgctataacg cgttctttca gcctgccgag
     1981 ggcgttcact ggttgcattg aaaccgtttc gtcttatcag cctacagacc gcgcctgacc
     2041 tgtcgggtgg ataagacgtt cacgccgcat ccggtaatct ttgccctgtt ccgtttcgat
     2101 cttaactcca gctcaagcaa caccgtaaaa gttgcaaaaa cgttaaatgc gtcacacatt
     2161 tcaatgttaa agtggtcggc ttttcccctg aaacatgcca cgggtaacac catgagtgaa
     2221 tttattgccg aaaaccgcgg cgcggatgcc atcacccgac cgaactggtc agccgttttc
     2281 tcggtggcgt tttgtgtcgc ctgtctgatt atcgttgagt ttttgcccgt cagtttgttg
     2341 acgccaatgg cccaggattt aggcatttcg gaaggggttg ccgggcaatc ggtgaccgtg
     2401 accgcctttg tggcaatgtt tgccagtttg tttattaccc agacaattca ggctactgac
     2461 cgccgctacg ttgttatttt gtttgccgtt ttgctgacgc tctcctgctt gctggtttcc
     2521 tttgctaact cattcagttt gcttttaatc ggtcgtgcct gtctggggct ggcgctgggc
     2581 gggttctggg cgatgtcggc gtcgctgacc atgcgtctgg tgccgccgcg tacggtgccg
     2641 aaggcgctgt cggtgatctt cggcgcggtt tctattgcgc tggtgattgc cgcgccgttg
     2701 ggcagttttt taggcgagct tatcggttgg cgcaatgtct ttaatgcggc ggcggtgatg
     2761 ggcgtgctgt gtattttctg gattatcaaa tcattgcctt cactgccagg cgaaccctcg
     2821 catcagaaac aaaatacttt ccgcttatta caacgtccgg gtgtgatggc agggatgatc
     2881 gccatcttca tgtctttcgc cgggcagttt gctttcttca cgtatattcg cccggtgtat
     2941 atgaacctgg cgggattcgg cgtggatggc ttaacgctgg tgctgttgag ttttggtatc
     3001 gccagcttta ttggtacgtc gctttcgtcg ttcattctta aacgttcggt aaaactggcc
     3061 ttagcaggcg cgccgttaat actggctgtg agtgcgttgg tactgacgtt gtggggaagc
     3121 gataaaatcg ttgctaccgg cgtggcgatt atctgggggc taacttttgc attggttccc
     3181 gtcggctggt caacgtggat cacccgctcg ctggccgatc aggcagaaaa agccgggtct
     3241 attcaggtgg cggttattca gcttgctaat acctgtggcg cggcaatcgg cggttatgcg
     3301 ctggataata ttggtctgac ttcgccgttg atgttgtccg gcacattgat gttgctgact
     3361 gcattgttgg ttactgcaaa ggtgaaaatg aagaaatcct gatatcaggt aaagaagcgt
     3421 tggtcagtag ttgttgctgt tcttacatat ccgggcattc taccttaccc aacgcttccc
     3481 aataattgct ctgattatta cgttccatcg ccacgatggc ctcgcgtata tgttgtttat
     3541 tgttttcatc tgacaacatt aacttttccc acgcttcatc tgataatgac gcctgtggcg
     3601 tgcaaatttt acgtaaatct tgtaataccg tggttttctc ttttgcggac tgcagtgagc
     3661 taaaaggcga ctcggcgtat acgatgccta gcatagcgaa aaaagtgaaa ataagcagta
     3721 gtgtcgttgg tttcatggat tcaacctttt acaaatgtgt atcgaatgaa gcataagtgt
     3781 agtttgcttt catcgctctc atcatgctgt acgaaacgcg cttgcgtggt cagtaagaag
     3841 tgccagactt tatattccac ttttattcct ttttattctt atcgatagcg tttcgttttt
     3901 taaaccgcag cgaccttacc gctatagtca ggtaatcatt aataaaagga taaaaaaatg
     3961 aaactgacaa cacatcatct acggacaggg gccgcattat tgctggccgg aattctgctg
     4021 gcaggttgcg accagagtag cagcgatgca aaacacatta aagttggcgt tataaatggc
     4081 gcagaacaag atgtcgcgga agtcgctaaa aaggtggcaa aagagaaata tggcctcgat
     4141 gttgaactgg taggatttag cggttcgttg ctaccgaacg atgcgacaaa tcatggcgaa
     4201 cttgatgcga atgtcttcca gcatcgaccc tttcttgaac aagataatca ggcgcatggt
     4261 tataaactgg tagcggtggg aaatacgttt gtcttcccta tggcgggtta ttccaaaaaa
     4321 atcaaaaccg tggcgcaaat aaaagaaggc gcgacagtgg cgatccccaa cgacccgacc
     4381 aaccttggtc gcgcactttt actgttgcaa aaagagaagc taattactct gaaagaggga
     4441 aaagggttat tacctaccgc gctggatata accgataatc ctcgtcattt gcagattatg
     4501 gaactcgagg gggcgcagct cccgcgcgta ctggatgatc ccaaagttga tgtagcgatt
     4561 atcagcacca cttacattca gcagacaggg ctttctccgg tgcacgacag cgtatttatt
     4621 gaagataaga attcgccgta tgtgaatatt ttggtggcac gggaagataa taagaatgca
     4681 gaaaacgtga aggaatttct gcaatcttat caatcacccg aagtcgctaa agcggcagaa
     4741 accattttta acggtggcgc ggtgcctggc tggtaattct cactcatgcc gcgccggacg
     4801 atttattttt ctggcgcggc gacgggaatc cattgaaatc aaaatcaccg atgacagaat
     4861 cagcagcgtt cccagccagt ccggtaatgt gaacgtgatc cccagcaaca gcaaagagag
     4921 tagcgcgcta cttaatggtt ctgcacagct caaaatgctg gcttttggac cgccaattaa
     4981 ttgtgctcct ttcaggtaca gactaaatgt cagggacgta ccaatgacca ccaaataaaa
     5041 aaacgccaga atcaaactgc cgttaacgac aaagtttgtt ccttgtctgg cataaaaagg
     5101 caacagaatc agaccgccaa tcagcatact ccagccgacg actggtaacg tgccatagcg
     5161 ggcaattagc gttgagggat aggtggtata gaatgcagca gcaaatgccg aggcaatgcc
     5221 ccagaacaac gcggcaggag agatcgataa tgacgtcgga ttaccgtgtg tcaccaataa
     5281 aaaagtcccg accagcgatg tcaaaatagc gcagaaaacc agaatgcccg ggcgcgattt
     5341 acgcaccagt gagaaccagg cgacgataat cgtcggtgag aggaattgca gcaccgttgc
     5401 cgtggctgcg ttcgattttt cgatggttag caaaaaagtg agctgtacag ttagcgcgcc
     5461 aaccacggaa aaaatcagca ggctaatggc atctttatga ttgttaataa tagaaaagat
     5521 tttatcgcca tgaacaaatg acagcgtcag tagaatcaaa ccggcgaata tcaaacgcgt
     5581 catagtcaaa aactgcg
//
LOCUS       DAZFQD010000214         5543 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.214, whole genome shotgun sequence.
ACCESSION   DAZFQD010000214 DAZFQD010000000
VERSION     DAZFQD010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5543
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.214"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(33..770)
                     /gene="bamD"
                     /locus_tag="ACHKTW_004899"
     CDS             complement(33..770)
                     /gene="bamD"
                     /locus_tag="ACHKTW_004899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HFO6465771.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRVEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            905..1885
                     /gene="rluD"
                     /locus_tag="ACHKTW_004900"
     CDS             905..1885
                     /gene="rluD"
                     /locus_tag="ACHKTW_004900"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HFO6465772.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCNKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISMLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEENKDEVDWL"
     gene            1882..2613
                     /gene="yfiH"
                     /locus_tag="ACHKTW_004901"
     CDS             1882..2613
                     /gene="yfiH"
                     /locus_tag="ACHKTW_004901"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphorylase YfiH"
                     /protein_id="HFO6465773.1"
                     /translation="MSKLIVPQWPLPKGVAACSSSRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAAHAGWRGLCAGVLEETVSCFADNPENILAWLGPAIGPR
                     AFEVGVEVREAFMAVDAKASAAFIQHGDKYLADIYQLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            2743..5316
                     /gene="clpB"
                     /locus_tag="ACHKTW_004902"
     CDS             2743..5316
                     /gene="clpB"
                     /locus_tag="ACHKTW_004902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFO6465774.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKAAGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
BASE COUNT         1369 a         1332 c         1508 g         1334 t
ORIGIN      
        1 caagggctgc cgttttgtat ttcaggtttc tgttatgtat tgctgctgtt tgcggcgatg
       61 attttcgcta ctttttcagc ttgcgcattc atctgcatct gacggtatgc attttccatc
      121 agcggcagcg catcacgcgt agcctgggta tccgggtagt cgcgcaacat tccttctacg
      181 cggttaacga cggcaaccca tgcgccacgt tctgtatagt actcggccac ggagtattca
      241 tatttcgcca gacgatcttt caggaatacc agacgtttgg tggcatcggt ggtgtactga
      301 ctgtttggat agccgcgcac cagtttggaa aagtcactaa acgcagctcg tgcatgttga
      361 ggatcgcgat cgctacggtc aacgccaaag aacccttgca gcgcactgtc atccagcgcc
      421 atattggtca ggccacgcat gtacatgaca taatcgatat tcggatgggt cgggttaagg
      481 cgaataaaac gatcgatggc agcctgcgct aacggcaaat cggcgttttt atagtaggcg
      541 tagatgagat ccagctgcac ctgctgcgaa tacggaccaa acggatagcg attatctaac
      601 gcttccagtt gcgttattgc ctgtctccag ttaccgtcct gcagcttttg ttgtgcagtc
      661 gcgtaaattt catttggcgg attatcaggt acttcttcct ttgaccccga gcaacccgcc
      721 aaaaacaggc ttagtgtggc ggctgccacc agatatttca tgcgcgtcat gacgttttga
      781 ctttcctcaa aatgtaatac gggagattct ctgttcctgc tcccggttaa gaccagctac
      841 aatagcacac tatattaaac ggcaaagccg taaaacccca acgataaacg aagaagcagt
      901 atatatggca caacgagtac agctcactgc aacggtgtcc gaaaaccaac tcggtcaacg
      961 cttagatcag gctttggccg aaatgttccc ggattattca cgttcgcgaa taaaagaatg
     1021 gatcctcgac cagcgcgtgc tggtaaacgg caaagtttgt aataagccga aagaaaaagt
     1081 attgggtggc gagcaggttg ccatcaacgc tgagattgaa gaagaagcgc gttttgaacc
     1141 gcaggatatc ccgctggata tcgtctatga agatgaagat atcatcgtca ttaataaacc
     1201 gcgcgacctg gtggtacatc ctggcgcggg taacccggat ggcacggtac tgaatgcgtt
     1261 gcttcattac tatccgccca ttgccgatgt accacgtgcg ggcatcgtcc atcgtctgga
     1321 taaagacacc actggcctga tggttgtggc aaaaaccgtt ccggctcaga cgcgtttggt
     1381 cgaatctttg caacggcgtg aaattactcg tgagtatgaa gcggtggcga ttggtcatat
     1441 gaccgctggc ggtacagtag atgaacccat cagccgccac ccgaccaaac gtacccacat
     1501 ggcggtgcat ccgatgggca aaccggcggt gactcactat cgcatcatgg aacacttccg
     1561 tgtgcacacg cgtctgcggt tgcgtctgga aactggacgt acgcaccaga tccgcgtgca
     1621 tatggcccat atcactcatc cgctggtggg cgatccggtt tatggtggcc gtccgcgtcc
     1681 gccaaaaggt gcttcggaag catttatctc catgctgcgt aagtttgacc gccaggcgct
     1741 tcatgcaacc atgctgcgtc tttatcaccc gatctccggc atcgaaatgg aatggcatgc
     1801 gcctattcca caagatatgg tggagctgat tgaggtgatg cgcgccgatt tcgaagagaa
     1861 taaggatgaa gtggactggt tatgagtaag ctgattgtcc cgcagtggcc gctaccaaaa
     1921 ggtgttgcgg cctgtagctc ttctcgtatc ggcggcgtga gcttgccccc gtatgactcg
     1981 ctcaacctcg gtgcccattg tggcgataac ccggatcacg ttgaggagaa tcgcaagcga
     2041 ctttttgctg cgggcaattt gccttctaaa ccggtctggc ttgagcaggt acacggcaaa
     2101 gatgtgctta agcttactgg cgaaccttat gcctcaaaac gagcagatgc ctcttatagc
     2161 aatacgcccg gcacggtttg cgcagtgatg actgccgact gcctccctgt gctgttttgc
     2221 aatcgagcgg gaacggaagt cgccgccgct catgctggct ggcgtggact gtgcgcaggc
     2281 gtgctggaag agacggtttc ctgttttgct gataatccgg aaaatattct cgcctggtta
     2341 gggccggcaa ttggtccacg cgcgttcgaa gtgggggtgg aggttcgcga ggcgtttatg
     2401 gcagtagacg ctaaagcaag tgcagctttc attcagcatg gtgataagta tctggcggat
     2461 atttatcagc ttgcccggca gcgtctggcg aacgtgggtg ttgagcaaat tttcggcggc
     2521 gaccgttgta catatacgga aaatgagact ttcttctctt atcgtcgcga caagaccacc
     2581 ggtcgtatgg caagtttcat ttggctgata taacctaaag aatcaagacg atccggtacg
     2641 cgtgattttc ttttcacatt aatctggtca ataaccttga ataattgagg gatgacctca
     2701 tttaatctcc agtagcaact ttgatccgtt atgggaggag ttatgcgtct ggatcgtctt
     2761 actaataaat tccagcttgc tcttgccgat gcccaatcac ttgcactcgg gcacgacaac
     2821 caatttatcg aaccacttca tttaatgagc gccctgctga atcaggaagg gggttcggtt
     2881 agtcctttat taacatccgc tggcataaat gctggccagt tgcgcacaga tatcaatcag
     2941 gcattaaatc gtttaccgca ggttgaaggt actggtggtg atgtccagcc atcacaggat
     3001 ctggtgcgcg ttcttaatct ttgcgacaag ctggcgcaaa aacgtggtga taactttatc
     3061 tcgtcagaac tgttcgttct ggcggcactt gagtctcgcg gcacgctggc cgacatcctg
     3121 aaagcagcag gggcgaccac cgccaacatt actcaagcga ttgaacaaat gcgtggaggt
     3181 gaaagcgtga acgatcaagg tgctgaagac caacgtcagg ctttgaaaaa atataccatc
     3241 gaccttaccg aacgagccga acagggcaaa ctcgatccgg tgattggtcg tgatgaagaa
     3301 attcgccgta ccattcaggt gctgcaacgt cgtactaaaa ataacccggt actgattggt
     3361 gaacccggcg tcggtaaaac tgccatcgtt gaaggtctgg cgcagcgtat tatcaacggc
     3421 gaagtgccgg aaggattgaa aggccgccgc gtactggcgc tggatatggg cgcgctggtg
     3481 gctggggcga aatatcgcgg tgagtttgaa gaacgtttaa aaggcgtgct taacgatctt
     3541 gccaaacagg aaggcaacgt catcctgttt atcgacgaat tacataccat ggtcggcgcg
     3601 ggtaaagccg atggcgcaat ggacgccgga aacatgctga aaccggcgct ggcgcgtggt
     3661 gaattgcact gcgtaggtgc cacgacgctt gacgaatatc gccagtacat cgaaaaagat
     3721 gctgcgctgg aacgtcgttt ccagaaagtg tttgttgccg agccttctgt tgaagatacc
     3781 attgcgattc tgcgtggcct gaaagaacgt tacgaattgc accaccatgt gcaaattact
     3841 gacccggcaa ttgttgcagc ggcgacgttg tctcatcgct acattgctga ccgtcagctg
     3901 ccggataaag ccatcgacct gatcgatgaa gcagcatcca gcattcgtat gcagattgac
     3961 tcaaaaccag aagaactcga ccgactcgat cgtcgtatca tccagctcaa actggaacaa
     4021 caggcgttaa tgaaagagtc tgatgaagcc agtaaaaaac gcctggatat gctcaacgaa
     4081 gaactgagcg acaaagaacg tcagtactcc gagttagaag aagagtggaa agcagagaag
     4141 gcatcgcttt ctggtacgca gaccattaaa gcggaactgg aacaggcgaa aatcgctatt
     4201 gaacaggctc gccgtgtggg ggacctggcg cggatgtctg aactgcaata cggcaaaatc
     4261 ccggaactgg aaaagcaact ggaagccgca acgcagctcg aaggcaaaac tatgcgtctg
     4321 ttgcgtaata aagtgaccga cgccgaaatt gctgaagtgc tggcgcgttg gacggggatt
     4381 ccggtttctc gcatgatgga aagcgagcgc gaaaaactgc tgcgtatgga gcaagaactg
     4441 caccatcgcg tcattggtca gaacgaagcg gttgatgcgg tatctaacgc tattcgccgt
     4501 agccgtgcgg ggttggcgga tccaaatcgc ccgattggtt cattcctgtt cctcggccca
     4561 acgggtgtgg ggaaaactga gctttgtaag gcgctggcga actttatgtt tgatagcgac
     4621 gaggcgatgg tccgtatcga tatgtccgag tttatggaga aacactcggt gtctcgtctg
     4681 gttggtgcgc ctccgggata tgtcggttat gaagaaggtg gctacctgac cgaagctgtg
     4741 cgtcgtcgtc cgtattccgt catcctgctg gatgaagtgg aaaaagcgca tccggatgtc
     4801 ttcaacattc tgttgcaggt actggatgat ggtcgtctga ctgacgggca ggggagaacg
     4861 gtcgacttcc gtaatacggt tgtcattatg acctctaacc tcggttccga tttgattcag
     4921 gaacgcttcg gtgaactgga ttatgcgcac atgaaagagc tggtgctcgg tgtggtaagc
     4981 cataacttcc gtccggaatt cattaaccgt attgatgaag tggtggtctt ccatccgctg
     5041 ggtgaacagc acattgcctc gattgcgcag attcagttga aacgtctgta caaacgtctg
     5101 gaagaacgtg gttatgaaat ccacatttct gacgaggcgc tgaaactgct gagcgagaac
     5161 ggttacgatc cagtctatgg tgcacgtcct ttgaaacgtg ctatccagca gcagatcgaa
     5221 aacccgctgg cacagcaaat actgtctggt gaattggttc cgggtaaagt gattcgcctg
     5281 gaagttaatg aagaccggat tgtcgccgtc cagtaaatga taaaacgagc ccttcggggc
     5341 tcgtttttgt ctataagtta gacggaaaag actatattta agatgttttg cctgaaaaat
     5401 gagcgaacga taaagttttt atatttttcg cttgtcaggc cggaataact ccctataatg
     5461 cgccaccact gacacggaac aacggcaaac acgccgccgg gtcagcgggg ttctcctgag
     5521 aattccggca gagaaagcaa aaa
//
LOCUS       DAZFQD010000215         5508 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.215, whole genome shotgun sequence.
ACCESSION   DAZFQD010000215 DAZFQD010000000
VERSION     DAZFQD010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5508
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.215"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..550)
                     /locus_tag="ACHKTW_004903"
     CDS             complement(<1..550)
                     /locus_tag="ACHKTW_004903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFO6465775.1"
                     /translation="MRADKSLSPFEIRVYRHYRIVHGTRVALAFLLTFLIIRLFTIPE
                     STWPLVTMVVIMGPISFWGNVVPRAFERIGGTVLGSILGLIALQLELISLPLMLVWCA
                     AAMFLCGWLALGKKPYQGLLIGVTLAIVVGSPTGEIDTALWRSGDVILGSLLAMLFTG
                     IWPQRAFIHWRIQLAKSLTEYNR"
     gene            complement(748..1221)
                     /gene="sbmC"
                     /locus_tag="ACHKTW_004904"
     CDS             complement(748..1221)
                     /gene="sbmC"
                     /locus_tag="ACHKTW_004904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor SbmC"
                     /protein_id="HFO6465776.1"
                     /translation="MNYEIKQEEKRTVAGFHLVGPWEQTVKKGFEQLMMWVDNKNIVP
                     KEWVAVYYDNPDETPAEKLRCDTVVTVPGYFTLPENSEGVILTEITGGQYAVAVARVV
                     GDDFAKPWYQFFNSLLQDSAYEMLPKPCFEVYLNNGAEDGYWDIEMYVAVQPKHH"
     gene            complement(1340..2506)
                     /gene="dacD"
                     /locus_tag="ACHKTW_004905"
     CDS             complement(1340..2506)
                     /gene="dacD"
                     /locus_tag="ACHKTW_004905"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase DacD"
                     /protein_id="HFO6465777.1"
                     /translation="MKRRLIIAASLFVFNLSSGFAAENIPFSPQPPEIHAGSWVLMDY
                     TTGQILTAGNEHQQRNPASLTKLMTGYVVDRAIDSHRITPDDIVTVGRDAWAKDNPVF
                     VGSSLMFLKEGDRVSVRDLSRGLIVDSGNDACVALADYIAGGQRQFVEMMNNYAEKLH
                     LKDTHFETVHGLDAPGQHSSAYDLAVLSRAIIHGEPEFYHMYSEKSLTWNGITQQNRN
                     GLLWDKTMNVDGLKTGHTSGAGFNLIASAVDGQRRLIAVVMGADSAKGREEEARKLLR
                     WGQQNFTTVQILHRGKKVGTERIWYGDKENIDLGTEQEFWMVLPKAEIPHIKAKYTLD
                     GKELTAPISAHQRVGEIELYDRDKQVAHWPLVTLESVGEGGMFSRLSDYFHHKA"
     gene            2715..4142
                     /gene="sbcB"
                     /locus_tag="ACHKTW_004906"
     CDS             2715..4142
                     /gene="sbcB"
                     /locus_tag="ACHKTW_004906"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008310 - single-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HFO6465778.1"
                     /translation="MMNDGKQQSTFLFHDYETFGTHPALDRPAQFAAIRTDSEFNVIG
                     EPEVFYCKPADDYLPQPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGY
                     NNVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDG
                     LPSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLM
                     ALIDVPQMKPLVHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMVDLAGDISPLLELDS
                     DTLRERLYTAKTDLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNL
                     KILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLP
                     ALDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYADELQML
                     AQQYADDKEKVALLKALWQYAEEIV"
     gene            complement(4185..4412)
                     /gene="tsuB"
                     /locus_tag="ACHKTW_004907"
     CDS             complement(4185..4412)
                     /gene="tsuB"
                     /locus_tag="ACHKTW_004907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate utilization sulfurtransferase
                     TsuB/YeeD"
                     /protein_id="HFO6465779.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(4426..5484)
                     /gene="tsuA"
                     /locus_tag="ACHKTW_004908"
     CDS             complement(4426..5484)
                     /gene="tsuA"
                     /locus_tag="ACHKTW_004908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate utilization transporter TsuA/YeeE"
                     /protein_id="HFO6465780.1"
                     /translation="MFSMILSGLICGALLGFVMQRGRFCLTGGFRDMYIVKNNRMFYA
                     LLIAISVQSVGVFALIQAGLLTYEAGAFPWLGTVIGGYIFGLGIVLAGGCATGTWYRA
                     GEGLIGSWIALFTYMVMSAVMRSPHASGLNQTLQHYSTEHNSIAETFNLSVWPLVAVL
                     LVITLWVVMKELKKPKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLS
                     EATGRMFGLGITSPTANILQFLVAGDVKYINWGVFLVLGIFVGSFIAAKASREFRVRA
                     ADAQTTLRSGLGGVLMGFGASIAGGCSIGNGLVMTAMMTWQGWIGLVFMILGVWTASW
                     LVYVRPQRKARLATAAAN"
BASE COUNT         1492 a         1479 c         1248 g         1289 t
ORIGIN      
        1 cccgattata ctcggtcaga cttttcgcaa gttgaatgcg ccagtggatg aacgcccgtt
       61 gtggccagat accggtaaac aacattgcca gtaaagagcc gaggatcaca tcgccgcttc
      121 gccataacgc cgtatcaatt tcacctgtcg gggaaccaac aacaattgcc agcgtcaccc
      181 caatcaataa accttgatac ggtttcttgc ccagcgccag ccaaccgcaa agaaacatcg
      241 ccgccgcgca ccagactaac atcagcggta acgaaattaa ctccagttgc agagcgataa
      301 gacctaaaat cgaacccaac accgtaccgc caatacgttc gaaggcgcga gggacaacgt
      361 taccccagaa cgagattggc cccataatca ccaccatggt gaccagcggc caggtgcttt
      421 ccgggatagt aaacaggcgg ataatgagaa aagtgagcag gaatgccagc gcgacccgag
      481 taccatgcac aatgcggtaa tggcggtata cccggatttc aaacgggctt aatgacttat
      541 cggcacgcac gcagaaactc cgtaatgaag attaaaagcg ctcataactc atctgtcggc
      601 cagaaggcgc acctctgatt tatccgacgg acataccggg aacagtgtaa tttcccggca
      661 ggagcgaaaa atttgaaagg cgcatcttac tcttttcgct ttcaaaaaaa gagtggtctt
      721 cgcgtcaaca caccgccctg agatgaatta gtgatgtttt ggctgcaccg caacatacat
      781 ttcgatatcc cagtacccat cttccgcgcc attgttcaag taaacctcga agcagggctt
      841 tggtaacatt tcataagcac tgtcctgcaa gagactatta aagaactgat accagggttt
      901 agcaaaatca tcaccgacta cacgagctac cgccaccgca tactgaccac ctgtaatttc
      961 tgtcagaatg acgccctcac tgttttcggg aagcgtaaag taacccggca ccgtcacgac
     1021 ggtgtcgcag cgtaattttt cggcgggtgt ttcatctgga ttgtcgtaat agacagcaac
     1081 ccactccttc ggcacaatat ttttgttatc tacccacatc atcaactgct caaagccttt
     1141 ctttaccgtc tgttcccacg ggccaacgag atggaaacct gcaacggtac gtttctcttc
     1201 ctgcttaatc tcgtagttca tgacgcctcc attaacactg tttttatata tagtatagtt
     1261 gcaatattaa aaccacaagg aatgagtgtt gattatgcga gcagactcgc actcctgcca
     1321 gtctgctgca aaagaaaggt caggccttat ggtggaaata atcactcagg cgggaaaaca
     1381 tgccgccttc cccgacagat tccagggtaa ccagcggcca gtgcgccacc tgtttatcac
     1441 ggtcgtaaag ttcaatttcc cctacccgct gatgggcgct aattggcgcg gtgagctctt
     1501 taccatcaag ggtatatttg gctttgatat gtggaatttc ggctttcggt agcaccatcc
     1561 agaactcttg ttccgttccc aggtcgatat tttctttatc gccataccag atgcgttccg
     1621 ttccgacctt tttcccacgg tgcaaaattt gcaccgtagt aaagttttgt tgcccccaac
     1681 gcagtaattt tcttgcctct tcctcacgac cttttgcact gtcagccccc attaccactg
     1741 caatgagacg acgctgccca tctacagccg aagcaatgag attaaacccg gcaccagaag
     1801 tatgacccgt tttcaggccg tcaacattca tggttttatc ccacaacaac ccgttacggt
     1861 tttgctgggt gataccgttc caggtgagac ttttctcact gtacatatga taaaactcgg
     1921 gctcgccgtg gatgatagcg cgagaaagca cagctaaatc ataagccgag ctatgctggc
     1981 caggtgcatc cagaccatgc actgtttcaa aatgcgtatc cttgagatgc agcttctcgg
     2041 catagttgtt catcatttca acaaactgcc gttgcccacc ggcaatatag tcagccagag
     2101 caacacaagc gtcatttccg gaatccacaa ttaaaccacg gcttaaatca cgtaccgata
     2161 cgcgatcgcc ctctttcaaa aacatcagtg aagaaccgac aaacaccgga ttatctttcg
     2221 cccacgcatc gcgccccacg gtgacaatat cgtctggcgt aatgcgatga ctatcgatag
     2281 cgcgatccac gacataaccc gtcatcagct ttgtcaggct ggcgggattg cgctgttgat
     2341 gctcattacc cgcggtgagg atctgaccgg tggtgtaatc catcaatacc caggacccgg
     2401 catgaatctc tggaggctga ggtgaaaaag gaatgttttc cgccgcaaaa ccagacgata
     2461 agttaaaaac gaacaaagaa gcagcaataa taagacggcg tttcaacagc aaaccctcag
     2521 gagtttcaaa tagctgttct ttttacggaa atacttatga actggctgga ataaagtgca
     2581 agaaaatgtg actaccctct catttttatc tgacatgatc tgttgccact cgctgccaaa
     2641 ttgtggcgct aaagctgatt agcacggtga tatttgatac tctggcagac agcagaaata
     2701 acggatttaa cctaatgatg aatgacggta agcaacaatc tacctttttg tttcacgatt
     2761 acgaaacctt tggcacgcac cccgcgttag atcgccctgc acagttcgca gccattcgca
     2821 ccgatagcga attcaatgtc atcggcgaac ccgaagtctt ttactgcaag cccgcggatg
     2881 actatttacc ccagcctgga gcagtattaa ttaccggtat taccccgcag gaagcacggg
     2941 cgaaaggaga aaacgaagcc gcgtttgccg cccgtattca ctcgcttttt accgtaccga
     3001 agacctgtat tctgggctac aacaatgtgc gtttcgacga cgaagtcaca cgcaacgttt
     3061 tttatcgtaa tttctacgat ccttacgcct ggagctggca gcatgataac tcgcgctggg
     3121 atttgttgga tgttatgcgt gcctgttatg ccctgcgccc ggaaggaata aactggcctg
     3181 aaaatgatga cggtctaccg agctttcgcc ttgagcattt aaccaaagcg aatggtattg
     3241 aacatagcaa cgcccacgat gcgatggctg atgtgtacgc cactattgcg atggcaaagc
     3301 tggtaaaaac gcgtcagcca cgcctgtttg attatctctt tacccatcgt aataaacaca
     3361 aactgatggc gttgattgat gttccgcaga tgaaacccct ggtgcacgtt tccggaatgt
     3421 ttggagcatg gcgcggcaat accagctggg tggcaccgct ggcgtggcat cctgaaaatc
     3481 gcaatgccgt aattatggtg gatttggcag gagacatttc gccattactg gaactggata
     3541 gcgacacatt gcgcgagcgt ttatataccg caaaaaccga tcttggcgat aacgccgccg
     3601 ttccggttaa gctggtgcat atcaataaat gtccggtgct ggcccaggcg aatacgctac
     3661 gcccggaaga tgccgaccga ctgggaatta atcgtcagca ttgcctcgat aacctgaaaa
     3721 ttctgcgtga aaatccgcaa gtgcgcgaaa aagtggtggc gatattcgcg gaagccgaac
     3781 cgtttacgcc ttcagataac gtggatgcac agctttataa cggctttttc agtgacgccg
     3841 atcgtgcagc aatgaaaatt gtgctggaaa ccgagccgcg taatttaccg gcactggata
     3901 tcacttttgt cgataaacgg attgaaaagc tgttgttcaa ttatcgggcg cgcaacttcc
     3961 cggggacgct ggattatgcc gagcagcaac gctggctgga gcaccgccgc caggtcttca
     4021 cgccagagtt tttacagggt tatgcagatg aattgcagat gctggcacaa caatatgccg
     4081 atgacaaaga gaaagtggcg ctgttaaaag cgctttggca gtacgcggaa gagatcgtct
     4141 aatttgaagc ctccgccgct ggtacggcgg aggacaatgt tgagtcaggc tttttgaacg
     4201 gtgatgctcc acgctgcatc gccaatctgc tgataatcgg tgatggcatg tccttcttct
     4261 gccgcccact gtgggattgc ttcagttgcc tgggtgcaat caaactcaat taccaactca
     4321 tcgccgctgg ccatttctgc cagtgctgct tttgcttcta tgagtggaaa cggacagacc
     4381 tgcgtcacca catccagctt tttaatcgcc atctttacac cttacttaat ttgccgcagc
     4441 agttgccagt cgcgccttac gctgcggtcg aacatacaca agccaggacg cagtccagac
     4501 tccgagaatc ataaatacaa ggccaatcca gccctgccag gtcatcattg cagtcataac
     4561 cagtccatta ccgatagagc aaccacctgc aatactggca ccgaagccca tcagtacacc
     4621 acctaacccg ctacgtaatg ttgtttgtgc atcagctgcg cgaacgcgga actcacggct
     4681 cgctttggct gcaataaatg accccacgaa gatccctaat accaggaaaa cgccccagtt
     4741 aatgtatttc acgtcacccg cgaccagaaa ttgcagaata ttggccgttg gggaagtgat
     4801 tcctaaccca aacatgcgtc cggttgcttc actcaggggc caggctaaaa gcgcaatcaa
     4861 accgatgagt acagcagtga caaagggatg ccagcgtttt tcaaacagaa tatgagcgat
     4921 cccggttcgg cgcggcggta aggtcgcgac tttgagtttt ggcttcttca actctttcat
     4981 caccacccag agcgttatca ccagcaaaac ggcaaccaac ggccacacag acaaattaaa
     5041 agtctcagca atagagttat gttcagtact gtagtgctgc aaggtttgat ttaaaccact
     5101 ggcatgtgga gaacgcatca ccgcactcat caccatataa gtgaaaagcg cgatccaact
     5161 gccgatcaat ccctcacccg cgcgatacca ggtcccggtg gcacatccac ccgccagaac
     5221 aattcccagc ccgaagatat acccacctat aacagtacca agccacggga acgccccggc
     5281 ttcgtaagtc agtaggcccg cctgaattaa cgcaaaaacc cccacgcttt gtaccgaaat
     5341 tgcaatcagc aaggcgtaaa acatgcgatt atttttcacg atatacatat cgcgaaaacc
     5401 acctgtcagg caaaaacgcc cacgctgcat gacaaatccc agcagagcac cacaaattag
     5461 cccgcttaat atcattgaaa acataaatta attaaccaga tgaatgtt
//
LOCUS       DAZFQD010000216         5446 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.216, whole genome shotgun sequence.
ACCESSION   DAZFQD010000216 DAZFQD010000000
VERSION     DAZFQD010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5446
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.216"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            303..1205
                     /locus_tag="ACHKTW_004909"
     CDS             303..1205
                     /locus_tag="ACHKTW_004909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HFO6465781.1"
                     /translation="MYFSGEPAQIAEIKRLASGAVTPFYRRATNEGIQLFLAGSAGLL
                     QTTEDVRFEPCPGLTAAGRGVVSPENIAFTRWLTHLQNGVLLDEQNCLMLHELWLQIG
                     TGQRRWEGLPDDVRDTITALFTAKRGDWCGFWSNEDVSVWWNRLCDNVLPEKTMPFDL
                     LTVLPTRLDVEVNGFNGGVLNGVPSAYHWYTEQYGVKWPCGYDLNISSQGENFIQVDF
                     DTPWCQPESDVVAELSRRFSCTLEHWYAEQGCDFCGWQLYERGELVDVLWGELEWSSP
                     TDDDELPEVTGPAWIVDNVAHYGG"
     gene            1202..1444
                     /locus_tag="ACHKTW_004910"
     CDS             1202..1444
                     /locus_tag="ACHKTW_004910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6465782.1"
                     /translation="MKYDGDGRATARFFSGKGCRRAPLFTAPADAARHKRCLWSVSRV
                     RRARDGRFYRSRLASVTVYASPSPFSDERPSSRFRE"
     gene            1542..2558
                     /locus_tag="ACHKTW_004911"
     CDS             1542..2558
                     /locus_tag="ACHKTW_004911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISShdy1 family transposase"
                     /protein_id="HFO6465783.1"
                     /translation="MTVVTVGIDLAKNVFAVHGVNAAGKAVLVKPLVRRAKLLELIAT
                     LPACLIGIEACTGAHYWAREFQKMGHTVRIMAPQLVSPYRMGGRHGKNDAADAAAICE
                     AVTRPNMRFVPIKSVEQQGQLFVHRAHQGYVSERTALINRIRGLLSELGIVLPQAFKA
                     FLSGVAQVLEDLPGYCNQVIGDLLNELYHIEEKIKNSEKLIQQLARDNSAAQLLMQFR
                     GIGPMTATAIVASIGNGHDFKNGRQFSAWLGLTPRQQSSGGKARLGRITKAGDSYLRT
                     LLVLGARSVLLGAAKNDDPVSRWALQLKERRGYGRALVAIAAKNARMCWAMLHLRDEF
                     IFPA"
     gene            3105..3179
                     /locus_tag="ACHKTW_004912"
                     /pseudo
     CDS             3105..3179
                     /locus_tag="ACHKTW_004912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052644.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3256..3939
                     /locus_tag="ACHKTW_004913"
     CDS             3256..3939
                     /locus_tag="ACHKTW_004913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HFO6465784.1"
                     /translation="MSRFVLGNCIDVMARIPDNAIDFILTDPPYLVGFRDRSGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWHRIDRFMAAWKHAGFSVAGHLVFTKNYT
                     SKSAYVAYRHECAYILAKGSPARPRNPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DDWFMPEAA"
     gene            3940..4161
                     /locus_tag="ACHKTW_004914"
     CDS             3940..4161
                     /locus_tag="ACHKTW_004914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6465785.1"
                     /translation="MNYAGHEKLRAEVAEVANAMCDLRTTMNEMEQRYSFNADTLPER
                     LVRQTLFRANRLLMEAYTEILELEACFKD"
     gene            4175..4609
                     /locus_tag="ACHKTW_004915"
     CDS             4175..4609
                     /locus_tag="ACHKTW_004915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HFO6465786.1"
                     /translation="MYGTCETLCRELAVKYPGDMPLMLVIWSPEEIQALADGMDISLS
                     DHEIRTVLARLEDIPEDQRTESGISSGVAMEIINNVSENRQVTVPAELLASLIQTAEQ
                     ALWKREWAARDHGLAVPECVTRRQAVINQARTLLKNNRHEND"
     gene            4655..5431
                     /locus_tag="ACHKTW_004916"
     CDS             4655..5431
                     /locus_tag="ACHKTW_004916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6465787.1"
                     /translation="MNENTTLNALICRHARNLLLAQGWPEETDVDQRNPNHPGWISIY
                     VLLDAPRLATLLINRHGGALPPLLASAIHKLTGTGAELVLSGSQWQSLPVLPADGTQV
                     SFPYAGEWLAEDEIRAVLDAVRDAVRCVSYQVAEDTRRIRAALTTTGQTLLTRQTRRF
                     RLVVKESDHPCWLDEDDENLPVVLDAILNRGARFSAVEMYLVSDCIEHILSSWLAWDV
                     LRIPDEPPRRWFDRGVLREVVREARNEIRSMADALAKIRK"
BASE COUNT         1223 a         1378 c         1569 g         1276 t
ORIGIN      
        1 gctgtatgcc tgctgtggag tgctgtgtcc agcttatcca cagcattttg tgcacggttc
       61 tgtggacaaa atacctggtt acccaggccg tgccggcacg ttaaccgggc tgcatccgat
      121 gcaagtgtgt cgctgtcgcc ggcctcctca cccggtcacg tttcgtcgtt cctcctccac
      181 gcgctgcggc ttcggggccg cacctgcatt cgtatgcggt cgcccggtta caggtgcggc
      241 acggcctgat ggaggccgca tgtgagagga gaattcccat gccaaactgg tgctcgaatc
      301 gtatgtattt ttctggtgaa ccggcacaga tcgctgagat taaacgactg gccagtggtg
      361 ccgtcacacc gttttatcgc cgcgccacaa atgaaggtat tcagctgttt ctggccggaa
      421 gtgccggact tctgcagacc actgaagatg tgcggtttga accatgcccc ggactgacgg
      481 ctgccgggcg tggcgttgta tcgccggaga atatcgcgtt cacccgctgg ctgacacacc
      541 tgcagaacgg tgtactgctg gatgaacaaa actgcctgat gctgcatgaa ctctggctgc
      601 agatcggtac tggacagcgt cgctgggaag gattaccgga cgatgtcagg gataccatca
      661 ccgcactttt caccgcaaaa agaggtgact ggtgtggctt ctggagtaac gaggatgtat
      721 cggtgtggtg gaaccgtctg tgtgacaacg tactgccgga aaaaaccatg ccgtttgacc
      781 tgctgacggt tctgccgacc cgcctggatg ttgaagtgaa tggcttcaac ggtggtgttc
      841 tgaacggtgt tccttctgca tatcactggt atacggaaca gtatggcgtg aagtggcctt
      901 gtgggtatga cctgaatatc agcagtcagg gggaaaactt cattcaggtg gatttcgaca
      961 cgccgtggtg tcagccggaa agcgacgttg ttgcagaatt aagccgccgt ttcagctgca
     1021 cgctggagca ctggtatgcc gaacagggct gtgatttctg tggctggcag ttgtatgagc
     1081 gcggagagct cgttgatgtg ctgtgggggg aactggaatg gtcttccccg acagatgacg
     1141 atgagctgcc ggaagtcacc ggacctgcgt ggatagtcga caatgtggcg cattatggcg
     1201 gatgaagtat gacggagacg ggcgggcaac cgcccgtttc ttttccggta aaggatgtcg
     1261 ccgggctcct ctttttactg cgccggctga cgcggcgcgg cataaacgct gcctgtggtc
     1321 agtgtcccgc gtccggcggg cacgggacgg gcgcttttac cgctcccggc tggcgtcggt
     1381 gacagtgtac gccagcccgt cgcccttttc tgatgaacgc ccttcaagcc gctttcgcga
     1441 atgaaaggga catcttattc ggtctttacg ctggggagtt actttggaag actgcgtcaa
     1501 cacgatgata agcgtacaaa gccattgtta agggatcctc tatgactgtt gttactgtcg
     1561 gtattgattt agctaaaaat gtgttcgctg tgcatggcgt taatgctgca ggaaaagctg
     1621 ttttagttaa gcctcttgtg cgtcgggcta aattgcttga attgattgca acacttccag
     1681 catgtctgat tggaattgaa gcatgtacag gtgcacatta ctgggcaaga gagttccaga
     1741 aaatggggca tacagttcga attatggcgc cgcaactggt ttctccatac cgtatgggtg
     1801 gccgtcatgg gaaaaatgat gctgcagatg ccgctgccat ttgtgaagct gttacccgtc
     1861 ctaatatgcg gtttgttccc ataaaatcag ttgagcagca agggcaatta tttgtacaca
     1921 gagctcatca gggatatgtc agtgagagaa cagcattgat taatcgcatc cggggattgc
     1981 tcagtgagtt aggtattgtt ctcccacagg cgtttaaagc gtttttatcc ggggtggctc
     2041 aggtccttga ggatttgccc ggttactgta atcaggttat tggtgatctt cttaatgagc
     2101 tttatcacat agaagaaaag attaaaaaca gtgaaaaact cattcagcaa cttgccagag
     2161 ataactcagc agcacaacta ctaatgcaat tcagggggat aggcccgatg actgcaacag
     2221 caatcgttgc cagcataggc aatggtcatg atttcaagaa cggacgtcag tttagtgcct
     2281 ggctggggct aacaccaaga caacaaagtt ccggggggaa agcacggtta gggagaataa
     2341 ctaaagccgg cgacagctat ctcagaacac ttcttgtttt gggggcgaga tctgtattgt
     2401 tgggagcagc taaaaatgat gatccggtca gtcgatgggc cctgcagtta aaagaaaggc
     2461 gaggatatgg acgagcgctt gttgcgatag cagcaaaaaa tgcacgtatg tgctgggcaa
     2521 tgttgcacct gagagatgaa ttcatatttc cagcctagtc tactgattat taatgagttt
     2581 atgaaattga tttccttcct tgcatgacga ctgcgcgatg atgatttaaa aggtttggac
     2641 ctgcgccatg gtttactcga gatatgcaag aggctgccat gtttatgtgg atatctgttt
     2701 cgcggatgga gtccatggcg tgcgtctttc gtcagggttc gttgctaaca acagcacgtt
     2761 aataaccgtt tataggctcg ctgtccgtaa ccttttactc tgactgtgca agttgtgtgc
     2821 agaaatcaat ctcatgatag ataaaagaat tattccactg ttgcacctgg agtgttcggt
     2881 gctcgctttg ctgcgctccg cctcaatcca gttgcaacag cgtttaatta aaagcattaa
     2941 ggcttgtgtc attgcgtact tgagctatgc aggagatttt tattggttct ctgtttcgcg
     3001 gatgaagtct ctgatatgag cctttctttc ataaaaatca ttctatttca tatagatata
     3061 ttaatttcat catcatcgtt gactctacaa cagatgtcct tataggcata gccttgccgt
     3121 caaaaagacg acggctgcgg tattccacgg tcggcctgac ccgttaccgg acgcggtgaa
     3181 cagcccacag gcagcgggga acgggcacca caggggtgcc ctcccggtgc cctctgtaag
     3241 agaaggagcg ttgttatgtc ccgttttgtc ctcggtaact gcatcgatgt tatggcccgt
     3301 attcctgata acgccattga tttcatactg actgacccgc cgtacctcgt cggttttcgt
     3361 gaccgttccg ggcgcaccat cgctggcgat aaaaccgatg aatggctgca accggcctgt
     3421 aatgaaatgt accgcgtact caaaaaagac gcgctgatgg tcagcttcta cggctggcac
     3481 cgtattgacc gctttatggc ggcctggaaa cacgccggat tcagcgtggc agggcacctc
     3541 gtgttcacca aaaactacac atccaaatct gcgtatgtcg cgtaccgcca cgaatgcgct
     3601 tacatcctgg caaagggcag tccggcccgg ccccgtaacc ctctgccgga tgtgctgggc
     3661 tggaaatatt caggcaatcg tcatcacccg acggaaaagc ccgttaccag cctgcaaccg
     3721 ctgattgaga gcttcacaca cccgaacgca attgtgctgg acccgtttgc aggcagcggc
     3781 tcaacctgcg tcgccgccct ccagtccgga cgccggtata tcggtatcga gctgcttgag
     3841 cagtatcacc gtgccgggca gcaacgcctt gccgccgtgc aacgggccat gcagcagggg
     3901 gccgcgaatg atgactggtt tatgccggag gctgcgtaaa tgaactatgc aggacacgaa
     3961 aaactgcgcg ccgaagtggc ggaggtggcc aatgccatgt gcgacctgcg tacaaccatg
     4021 aatgagatgg agcagcggta cagctttaat gccgacaccc tgccggaacg tctggtgcgt
     4081 cagacgctgt ttcgcgcaaa ccgcctcctg atggaggcat ataccgaaat tctggaactg
     4141 gaagcgtgct ttaaagattg agaaggagac gagaatgtac ggaacatgcg aaacgctatg
     4201 ccgggagctg gcagtaaagt atccgggaga catgccgctg atgctggtta tctggtcccc
     4261 ggaagagatt caggccctcg ctgacggaat ggatatttcc ctgtccgatc atgaaatcag
     4321 aaccgtcctg gcgcgcctgg aggacatccc ggaagaccag cggactgaat ccggtatttc
     4381 ttccggcgtg gcgatggaga tcatcaataa cgtgagcgaa aaccgccagg tgaccgtccc
     4441 tgctgaactg ctggcgtccc tgattcagac cgctgaacag gcattgtgga aacgtgaatg
     4501 ggccgcccgg gatcatggcc tcgccgtccc ggaatgcgtc acccgccgtc aggcggtgat
     4561 taatcaggcc cgcaccctgc tgaaaaacaa cagacacgaa aacgactgat gttatcgccg
     4621 cctccgggcg gcgattcagg gagaacgatt aatcatgaac gaaaacacaa cactgaacgc
     4681 actgatttgt cgtcacgccc gcaacctgct gctggcgcag ggctggccgg aagagacgga
     4741 tgttgaccag cggaacccga accatccggg ctggatcagc atttatgttc tgctggatgc
     4801 gccccggctg gcgacgttac ttatcaaccg tcacggcggc gcactgccgc cgctcctggc
     4861 ctccgccatt cacaaactga ccggaaccgg ggcggaactt gtactgtccg gcagtcagtg
     4921 gcagtcgctg ccggtacttc cggcagacgg aacgcaggtg tctttcccgt atgccggtga
     4981 gtggctggca gaggacgaaa tcagggcagt tcttgatgcg gtacgcgatg cagtacgctg
     5041 tgtcagttac caggtggcag aagatacgcg gcgtatccgg gcggcgctga ccaccaccgg
     5101 tcagacgtta ctgacccgcc agacgcgccg ctttcgcctg gtcgtgaagg agagcgatca
     5161 cccctgctgg ctcgatgagg acgacgaaaa cctgcccgtg gtgctcgacg ccatcctgaa
     5221 ccggggcgca cgtttttcgg cggtggaaat gtatctggtc agcgattgta ttgaacatat
     5281 cctgtccagt tggctggcct gggatgtgct gcgtataccg gatgaaccgc cccgccgctg
     5341 gtttgaccgt ggtgttctgc gggaggtggt ccgggaagcc cggaacgaaa tccgcagcat
     5401 ggcggatgcc ctggcaaaaa tccggaaatg atgacactgg cggggt
//
LOCUS       DAZFQD010000217         5403 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.217, whole genome shotgun sequence.
ACCESSION   DAZFQD010000217 DAZFQD010000000
VERSION     DAZFQD010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5403
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.217"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(119..1402)
                     /gene="aroA"
                     /locus_tag="ACHKTW_004917"
     CDS             complement(119..1402)
                     /gene="aroA"
                     /locus_tag="ACHKTW_004917"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFO6465788.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGVSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLGSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
     gene            complement(1473..2561)
                     /gene="serC"
                     /locus_tag="ACHKTW_004918"
     CDS             complement(1473..2561)
                     /gene="serC"
                     /locus_tag="ACHKTW_004918"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HFO6465789.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIECPSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            complement(2760..3452)
                     /locus_tag="ACHKTW_004919"
     CDS             complement(2760..3452)
                     /locus_tag="ACHKTW_004919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HFO6465790.1"
                     /translation="MKAFDLHRMAFDKVPFDFLGEVALRSLYTFVLVFLFLKMTGRRG
                     VRQMSLFEVLIILTLGSAAGDVAFYDDVPMVPVLIVFITLALLYRLVMWLMAHSEKLE
                     DLLEGKPVVIIEDGELAWSKLNNSNMTEFEFFMELRLRGVEQLGQVRLAILETNGQIS
                     VYFFEDDKVKPGLLILPSDCTQRYKVVPESADYACIRCSEIIHMKAGEKQLCPRCANP
                     EWTKASRAKRVT"
     gene            3582..5342
                     /gene="ycaO"
                     /locus_tag="ACHKTW_004920"
     CDS             3582..5342
                     /gene="ycaO"
                     /locus_tag="ACHKTW_004920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     accessory factor YcaO"
                     /protein_id="HFO6465791.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLTENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLEAEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFKKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
BASE COUNT         1393 a         1397 c         1275 g         1338 t
ORIGIN      
        1 acattgacaa gcgacgctac cattaatcaa aatgactcac aaggtccgaa aaaaaacgcc
       61 gacaatgtgc cgacgttttg ataaagaaag atttggctat ttattgcccg ttgttcattc
      121 aggctgcctg gctaatccgc gccaactgct cgaaataatc cggaaatgtt ttggccgtac
      181 atttgggatc aagaatcgtc actggtgtat ctgacaacgc caccagcgag aaacacatcg
      241 ccatccggtg atcattgtat gtcgcgatct cggcaaagtt cagtttttcc ggtggagtga
      301 tacgaatgta atcgtgcccc tcttctactt ccgcaccgac tttacgcagt tctgttgcca
      361 tcgcaaacag gcgatcggtt tctttaacac gccagttata gatattgcgc agcgtggtgg
      421 tgccttttgc aaataacgcc gccgtggcaa tggtcatcgc cgcatcggga atatggttca
      481 tatccatatc aatagcgttc agttcaccac gcgtgcagga aatataatca tcgccccagc
      541 aaatggtcgc gcccattttt tccagcacat cagcaaagcg aatatcaccc tgcatactgt
      601 tacgtccaat accggtcact tttacagtgc cgcctttgat tgctgctgct gccagaaagt
      661 aagaagccga agatgcatcg ccttcgacca aataagtacc cggagactga taagactgcc
      721 cgccttttac gacaaattgt tgatagtgct gattttcaat ttcaacacca aacgtcttca
      781 tcagattgag tgtgatgtcg atataaggtt tagaaaccag atcgccttta atacgaatca
      841 ccgtatcttc cggcgcaaga ggcgcagtca ttaacagtgc ggtgaggaat tggctggaaa
      901 cggagccatc aacgtcaacg ttgccgccag taaagccgcc ctgtaaacgc aacggcggat
      961 aattttcttg ttccaggtaa gtgatcttcg ccccgcccag gcgcagcgca tccaccagat
     1021 gaccaatcgg gcgttctttc atacgcggct caccggtcag cacaatatca ttgctaccca
     1081 gacaaagagc tgccgccagc ggacgcattg ccgttccggc gttaccgagg aataactcca
     1141 gggcaccttc tgcgtgtaat ggaccgccgt taccgataat ttcgcaacgc gtacgatcgg
     1201 ctgaaagcgt atagcttacc cctaacgctg ttaatgcatt cagcatatgg cgcacgtcat
     1261 cgctatccag cagattggtt aatactgttt tgccgtgtgc taatgccgcc agcaataaag
     1321 cgcggttaga aacgctcttg gaaccgggca gattaatagt gccatcgaca cgagcgatgg
     1381 gttgtaacgt cagggattcc atgaactcaa ctctctacaa cagaaataaa aaccccacag
     1441 gctggctgtg gggattaagc aaaatttcgg cattaaccgt gacggcgttc gaactcaacc
     1501 atgaagtctg tcagcgcttt aacgccttcc agcggcatgg cgttataaat agaagcgcgc
     1561 attccgccga ccacacggtg acctttcagt gcatgaaggc cagcagcaaa agactcttca
     1621 aggaacaatt tgtcaagcgc actgtccgcc aactggaacg gcacgttcat ccgcgaacgg
     1681 ttagctttcg ccacgtcatt gcggtagaaa tcgctgttat caatcacccc atatagcagt
     1741 tctgcttttt gctgattgat tttatccatt tcagctacac cgccgttcgc tttcagccat
     1801 ttaaagacca gaccagatag ataccaggca aatgtcggcg gcgtgttaaa catggagccg
     1861 ttatcgttga ggatggaata atccagaatc gacggacact cgatattcgc tttgcccagc
     1921 aaatcttcac gaacgatgac gattgtcagg ccagccgggc cgatattttt ctgcgcgcca
     1981 gcgtaaatca cgccataacg gctgacgtca atcggacggg aaagaatggt tgaagagaag
     2041 tcggcggcga ccaccacatc tttgccgaag tctggcgttt cgtcgatggc gataccgtcg
     2101 atggtttcat tcgggcagta atgcatataa gcagcattat cagagagttg ccattcacgc
     2161 attggcttaa ccgcgcgcag accatcaaca gtcactttgg cgtcaaagac attaggcgtg
     2221 cagtattttt tcgcttcttt aatggcactt gccgcccagt aaccggcatc aacataatct
     2281 gcggtggttt tatcaccgag aatattcagc ggtaccgcag caaactgacc gcgaccaccg
     2341 ccatggcaga ataatacctt gtagttggag gggacattaa gaagatcgcg aaaatccttc
     2401 tcggcttcct ctgcaacctg aatgaactct ttgccacggt gactcacttc catcaccgac
     2461 gtaccaagac cgttccagtc gcgcagttcc tgttgagcct gtttaagcac ctctgccggt
     2521 agcattgccg gaccagaact aaaattgaag atttgagcca tttcccctca ccacgttgcg
     2581 ttgctattaa cccgcggtca atcgattgcg accacaggta atacattatt cctgtggcta
     2641 tcggttttat cattcagtga cacacatcgc aatgagtaaa accgttgagg gcaaaaatgt
     2701 ggcttgcatc acacaaaaga atctctcgga ttattagcaa aaagccgatg tttttactgt
     2761 caggtcaccc gttttgcccg acttgccttc gtccattctg gatttgcaca gcgcggacat
     2821 aattgttttt cccccgcttt catatgaatg atttcactac aacgaataca ggcatagtcc
     2881 gccgactccg gcaccacttt gtaacgctgc gtacaatcac tgggtaaaat aagtaaaccc
     2941 ggtttcacct tgtcatcttc aaagaaatag acactgattt gcccgttggt ttcgagaatc
     3001 gccagacgta cctgccccag ctgctccacg ccacgcaatc gtagctccat aaagaactca
     3061 aattccgtca tgttggagtt attgagtttc gaccaggcca gctcgccatc ttcaataata
     3121 acaaccggct taccttccag taaatcttcc agtttttcac tgtgcgccat caaccacatt
     3181 accaggcggt ataacaacgc cagagtgata aagacgataa gtaccggcac catcggcaca
     3241 tcatcataaa acgccacatc tcccgccgct gaacccagcg tgagaatgat taacacttca
     3301 aacagcgaca tctgtcgcac accgcgtctt ccggtcattt tgaggaacag aaaaaccagt
     3361 acaaaggtat aaagactacg tagtgcaact tcgccaagga aatcaaaagg cactttatca
     3421 aatgccatac ggtggagatc gaacgccttc atttttatgc tcttatcgtg gtttatccgc
     3481 taaaagcata gtcaacagtt catcggcatg gcggcggtga tagcgccttt gagataaaac
     3541 ccgtattatt gcgcgctttc cgtacgacta aagtgatttt catgacgcaa acatttatcc
     3601 ccggcaaaga tgccgctctg gaagattcca tcgctcgctt ccagcaaaaa ctttcagacc
     3661 tcggctttca gattgaagag gcctcctggc tgaatcctgt gcctaacgtc tggtctgtac
     3721 atattcgcga caaagagtgc gcactgtgtt ttaccaacgg taaaggcgca accaaaaaag
     3781 cggcgctggc ttctgcactt ggtgaatatt tcgagcgtct ctcaaccaac tacttttttg
     3841 ctgatttctg gctgggcgaa accatcgcca acggtccgtt cgtgcattat cccaacgaaa
     3901 aatggttccc actgaccgaa aatgacgatg tgccagaagg actactcgat gaccgtctgc
     3961 gcgcgtttta tgatccggag aatgaactga ccggcagcat gctgattgac ctacaatccg
     4021 gtaacgaaga tcgtggtatt tgcggcctgc cgtttacgcg tcagtccgac aatcagaccg
     4081 tttatattcc gatgaatatc attggtaacc tgtacgtctc caacggtatg tccgcaggta
     4141 atacccgcaa cgaagcacgc gttcagggat tgtctgaagt tttcgaacgc tacgtgaaaa
     4201 accgcattat tgctgaaagc atcagcctgc cagagatccc ggcagacgtg ctggcgcgtt
     4261 acccagcagt agttgaagcc atcgaaacgc tggaagcgga gggtttccca atcttcgctt
     4321 atgacggttc gcttggcggc cagtatccgg tgatttgcgt ggtactgttc aatcctgcta
     4381 acggtacctg ctttgcctct ttcggtgcgc atcctgattt tggcgtagca ctggaacgta
     4441 ccgtgaccga gctgctgcaa ggtcgtggcc tgaaagattt ggatgtgttt actccgccaa
     4501 ccttcgatga tgaagaagtc gctgaacata ccaacctcga aacgcacttt atcgattcca
     4561 gcggtttaat ctcctgggac ctgttcaagc aggatgccga ttatccgttt gtggactgga
     4621 atttctccgg caccacggaa gaagagttcg ccacgctgat ggctatcttc aaaaaggaag
     4681 ataaagaagt ttatattgcc gattacgagc atctgggtgt ctacgcttgc cgcattatcg
     4741 tgcctggcat gtccgatatt tatccggctg aagatctgtg gctcgcgaat aacagtatgg
     4801 gcagccattt acgtgaaacg attctttcgc taccaggcag cgagtgggaa aaagaagatt
     4861 acctgaacct catcgagcaa ctggatgaag aaggttttga tgactttacc cgcgtgcgtg
     4921 agctgttggg tctggcgacc gggtcggata acggttggta caccctgcgt atcggtgaat
     4981 taaaagccat gctggcgctg gctggtggcg atctggaaca ggctctggtc tggaccgaat
     5041 ggacgatgga gtttaactca tcagtattta gtccggaacg cgccaactat tatcgctgcc
     5101 tgcaaacgtt gttattactg gcacaggaag aagatcgcca gccgctgcaa tatctgaatg
     5161 cgtttgttcg catgtacggc gcagatgccg tagaagccgc cagtgcggca atgagcggcg
     5221 aagcggcgtt ttacggcctg caaccggtag atagcgatct gcacgcgttt gctgcacatc
     5281 agtcgttgtt gaaggcctac gaaaagctgc agcgcgccaa agcagcattc tgggcaaaat
     5341 aaaatcaaat agcctacgca atgtaggctt aatgattagt ctgagttata ttacggggcg
     5401 ttt
//
LOCUS       DAZFQD010000218         5388 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.218, whole genome shotgun sequence.
ACCESSION   DAZFQD010000218 DAZFQD010000000
VERSION     DAZFQD010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5388
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.218"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            142..1494
                     /locus_tag="ACHKTW_004921"
                     /pseudo
     CDS             142..1494
                     /locus_tag="ACHKTW_004921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308049.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
     gene            1507..2466
                     /locus_tag="ACHKTW_004922"
     CDS             1507..2466
                     /locus_tag="ACHKTW_004922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HFO6465792.1"
                     /translation="MKWLLLITLSLYSFIVQSAPCALTNVGEQRGTYILKPLSMKGNL
                     TAEKIENTGNIIECTDLQETTNAIKVHFLTQNALRFKVGSRHYIINFPFEYDGKKIEL
                     PGKSSYNLEEMLSAINFTIPYSIASVSDSSNTADIFPGTPFPLLFEIEVSSCTGNNTN
                     CNSVRFNYNLNTILQVNLMTCGFEDQSIDTGRFVLSRINNENYQFHHIRFDCIQGEQG
                     ELVFEPSDVEYYFEPVTIQESGTSILKNELENSEDGAGQVGFQLSNDGTNEIQYGKSN
                     YYSFQHPHEGSNQIPLFIRPRTYGNNVSSGQIMSRVKIVVMYN"
     gene            complement(2504..3403)
                     /gene="nhaR"
                     /locus_tag="ACHKTW_004923"
     CDS             complement(2504..3403)
                     /gene="nhaR"
                     /locus_tag="ACHKTW_004923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HFO6465793.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAAR"
     gene            complement(3469..4635)
                     /gene="nhaA"
                     /locus_tag="ACHKTW_004924"
     CDS             complement(3469..4635)
                     /gene="nhaA"
                     /locus_tag="ACHKTW_004924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HFO6465794.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMIMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLALKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMAVGIL
                     CGIGFTMSIFIASLAFGNVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
     gene            5221..5373
                     /gene="mokC"
                     /locus_tag="ACHKTW_004925"
     CDS             5221..5373
                     /gene="mokC"
                     /locus_tag="ACHKTW_004925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HFO6465795.1"
                     /translation="MKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
BASE COUNT         1656 a         1199 c         1130 g         1403 t
ORIGIN      
        1 agcatactct gaaacatcct actctctcac tctaataatg gcaccttgct cttcttataa
       61 agaagggaat aattaagaat attaaacttt catcaacttc acatcaagaa attaaaaaat
      121 ttaaacatta atctaaataa taacaacctg atcacataat cgggtacgat tttatccgat
      181 aactataatg ctataactct gggaaatggt tggaatacac cgctgggtgc catctctttt
      241 tacgctgccc gatcgagtag taagttaaat aatgatacca gacacgaagg tacgagctat
      301 caggtcgcct ataataaata tctagtacaa acagccactc gatttagcgt ggccgcctgg
      361 cgttatgctt cgcaggatta caggacattc agcgaccatc tttacgaaaa tgataaacac
      421 tatcatcaga gtgattataa tgatttttat gatattggca gaaaaaatag cctttctgcc
      481 aatattatgc aacctttatc caataatctg ggaaatgtat cattaagtgc gctttggcgg
      541 aattactggg ggcgaagtgg aaatgctaaa gattaccaat tcagttactc caataactgg
      601 caacacatta gttatacttt ctctgccagc caatcttatg atgaaaataa taaagaagag
      661 gagcgtttta atctgtttat ctccatttct ttctactggg gggatgatat tgccaaaaca
      721 cgtcaccaaa ttaacttatc gaattcgacc tcattttcca aagatggcta ttcctccaac
      781 aatactggaa ttactggcat agccggtgaa catgatcagt taaattatgg tatatatgtt
      841 aatcagcaac aacaaaataa tgatacctcg cttggtacga atttaagctg gagaactccc
      901 atcgccataa tagatggcag ctatagtcat tctaaaaacg cctggcaaag tggtggaagt
      961 attagtagtg gattagttgt ctggtccggt ggtattaata tcactaacca gttatccgat
     1021 acatttgcaa ttctggatgc gcctggatta gaaggtgcac atattaatgg acaaaaatac
     1081 aatcgaacaa acagcaaagg ccaggttgtt tacgacctga taatacctca tcgtgaaaac
     1141 catctggtac ttgatatagc aaacagtgaa agtgaaacag aattgcaggg caatcgtcaa
     1201 attattgcac cttaccgtgg agcagtttct tatgtgcagt ttacaactga ccaacgtaag
     1261 ccctggtata tacaggcact gcgtcccgat ggttcgccat taacctttgg ctatgacgta
     1321 ctggatctcc aggaaaataa tattggagtc gttggccagg gtagtcgcct ttttattcgc
     1381 gtagatgaaa ttcccactgg aataaaagtt gctctcaatg atgaacagaa cttattctgt
     1441 actattactt ttcaacacgt tatcgatgaa aacaaaacat atatatgcca gtaaaaggag
     1501 tttactatga agtggctttt attaatcacc ttgagtctat attcttttat tgtgcaaagt
     1561 gctccctgtg cattgaccaa tgtcggtgaa caacgtggaa catatatact caagccactg
     1621 agtatgaaag gtaatctaac cgccgaaaaa attgaaaaca ccggaaatat cattgagtgt
     1681 acagacttgc aagagacaac caacgccatt aaagttcatt ttttaacaca gaacgcttta
     1741 aggtttaaag tcggaagtcg tcattatatt attaatttcc cctttgaata tgacggtaaa
     1801 aaaatagagc ttccagggaa gtcatcttat aatttagaag aaatgctatc tgcgataaat
     1861 ttcaccattc catattctat cgcatctgta tcagatagtt ctaacaccgc agacattttt
     1921 ccaggaactc cttttccttt gttgttcgaa attgaagtga gttcatgtac agggaataat
     1981 actaattgta atagcgtccg ctttaattat aatttaaata ctattttaca ggtaaattta
     2041 atgacctgtg gttttgaaga tcaatccatc gacactggac gatttgtact gtctcgtatc
     2101 aataatgaaa attatcagtt tcatcatata agatttgact gcatccaggg cgagcaaggt
     2161 gaattagttt ttgaacccag tgatgttgaa tattactttg aaccagtgac gatacaggag
     2221 tcaggaacca gcatattaaa aaatgagtta gaaaacagtg aggacggtgc gggtcaagta
     2281 ggctttcaac tctccaatga tggtacgaat gaaatccagt atggtaaaag caattactac
     2341 tcttttcagc atccccatga aggaagtaat cagattcctc tatttattcg cccgcgtaca
     2401 tatggaaata atgtctcaag cgggcaaata atgagccgag tcaaaatcgt ggttatgtac
     2461 aactaatcta tctcctccac tttaactttg ggggagctgc cgattaacgc gccgctggac
     2521 taaaaagcgc agaataatcc gtattgcaga ttcgctgtac cgccgggtgc tgaatcatcc
     2581 gctcagcaaa aatagcatgg tactcttcca tcacattctc gacgcgacca atttctacga
     2641 cagttttatc ggcataaaag tcatatgcat aaagcgttgg ggcaacgaag attgcattgt
     2701 gcattgcacc aaaagctttc atcaacgcgg catcatcaaa ctcgccgagg atttctacgt
     2761 ttaatccctg ggagttaaac cagttaagca atttgcgccc taacattgaa cgtcgcccag
     2821 gaatcaaaag tcgccgttct tccagacaag ccgggaacgg tttttctggt ggtggatttg
     2881 tacaccagaa actcacgcca cattcgccaa ttctcacgga gaacaggcct tcctgctgcg
     2941 tagagtctat cggacagtcg gaaatgatca tatccagttt atgctggctt aattgctcca
     3001 gtagcatttc gtgggtggat tcgaagcagc gaagatgaat cggctcgcct tctaccactg
     3061 cagcattcag cacgctactg accaggcgtt tggaaagtgc atcagccacg ccaacgtcaa
     3121 acaataaatt ggattctttg cgatagttca caatatccag catttcctgg cttaaggtga
     3181 acattttatc ggcatagcga tagaccagtt ctcccagctc gctgggttcg agaccacgtc
     3241 ccttgcgttt aaataatttg ccttgcaggc gctcttccag cgctcgaatc tgtccggtaa
     3301 tggtttgtgg agttaaataa agcgcctccg ctgcgccaac cacggaacct tctttataga
     3361 catgccagaa gtaatacaag tggttgtaat tgatatgaga catgctcatt tctctccctg
     3421 ataacaatga aaagggagcc gtttatggct ccccggtaaa ccgtcctgtc aaactgatgg
     3481 acgcaaacga acgcgtaacc agctgtatcc aattaccgcc gaagagatag aaccgaccag
     3541 gataccgagt ttcgcccagt taatcagttc tggatctacg ttaccaaagg ccaggctggc
     3601 aataaagata gacatagtaa aaccgatacc gcacaggatc cccaccgcca taatttgctg
     3661 ataagtcgtt ccctcaggca gatgcgccag tttcaaacgc agcgccaacc agcagaacag
     3721 actaatcccc agcggtttgc caatcagcaa gccagcgatg atccccaatg gcagaatgga
     3781 ggtcaagcca tccagcgtga caccttgcag tgaaacgcca gcattagcaa atgcaaacag
     3841 cggcaaaatc agatacgcca cccacgggtg taacacatgc tccagtcgct tcgctggaga
     3901 acgcccatgc ttctctttca aaggaataaa gaagccgaca attacccccg ccagggttgc
     3961 gtgaaccccc gatttcaaca ccgcagtcca caacactacg ccaaccagaa tatagacgcc
     4021 cgtgcggcgt acaccacaca gattcaatac cgcgagtacc gcaattgcta cagccgcgac
     4081 gccaagagag gccatcgata agtcattagt gtagaacaat gcgatgataa tgatggcccc
     4141 aagatcgtcg ataatagcca gagccatcaa aaagatcttc agcgctaacg gaacacgact
     4201 tcccaacagc gccagcacac caagtgcaaa ggcaatgtca gtagccgccg ggatcgccca
     4261 cccttcgcgg gtaatcggat cggcatagtt aaaggccaga tagagtaatg ccggaacaat
     4321 catcccacca atagcggcga taactggaaa tgcggcctgg cgtaagctgg ctagcgatcc
     4381 ttgcatcagt tcacgtttaa cttccagacc gactaacagg aaaaataccg ccatcagcgc
     4441 gtcatttatc cataacagca tgtttttgtt gatttcgagt gaaccaaccc ggagctgaac
     4501 cggcgtctcc agaaagtcgt gataccatcc actggttgcg ccgctattgg ccataatcat
     4561 cgccaggata gcggcaatga taagaataat gcctcccgag gcatcactgc taaagaatcg
     4621 atgcagatgt ttcacttttt atttctcttt caggtgaata gatcgttttt actattttac
     4681 cccgcacaaa tcatcgttaa aatcagatta tagtgtagga ataactcgtt ttttacgatc
     4741 cattgaagaa tttgcgccat cagcggtgag atgagttttt gacggctgta agcgctcacg
     4801 agtgacccta agcccagata atcatagctt aaggtgagga tttctgagag attaattagg
     4861 aaaagtaata attaatttgt tgtcagtcgg cgatgtttgt taggccaata tttcaaaaat
     4921 aatccagctt gtcgtcaata attgcgatga agagataaat taaagttaaa taattaaatc
     4981 attaaaaaat tacgccataa aaatgcgagg cgtcttcagg attagaatcg ataaaacaga
     5041 atgggttaaa gagagcgata taatagcggc gggtgcttga ggctgtctgt ctcaggcatt
     5101 agctgaacgg cagatagaga aaagccccga gtgatatttt accatcaacc cgaggccccc
     5161 tatatgctga acacatgtag agtgcctctt actgaccgta aggtcaagga gaagagagca
     5221 atgaagcagc ataaggcgat gattgtcgcc ctgatcgtca tctgtatcac cgccgtagtg
     5281 gcggcgctgg taacgagaaa agacctctgt gaggttcaca tccgaactgg ccagacggag
     5341 gttgctgttt tcacggctta cgaatccgag taagagcaac ggcgggga
//
LOCUS       DAZFQD010000219         5385 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.219, whole genome shotgun sequence.
ACCESSION   DAZFQD010000219 DAZFQD010000000
VERSION     DAZFQD010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5385
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.219"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            141..1475
                     /gene="garP"
                     /locus_tag="ACHKTW_004926"
     CDS             141..1475
                     /gene="garP"
                     /locus_tag="ACHKTW_004926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HFO6465796.1"
                     /translation="MILDTVDEKKKGVHTRYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGVIGFVLTALWIKLIHNPTDHPRMSAEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     ASTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            1491..2261
                     /gene="garL"
                     /locus_tag="ACHKTW_004927"
     CDS             1491..2261
                     /gene="garL"
                     /locus_tag="ACHKTW_004927"
                     /EC_number="4.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008672 - 2-dehydro-3-deoxyglucarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
                     /protein_id="HFO6465797.1"
                     /translation="MNNDVFPNKFKAALAAKQVQIGCWSALSNPISTEVLGLAGFDWL
                     VLDGEHAPNDISTFIPQLMALKGSASAPVVRVPTNEPVIIKRLLDIGFYNFLIPFVET
                     KEEAEQAVASTRYPPEGIRGVSVSHRANMFGTVADYFAQSNKNITILVQIESQQGVDN
                     VDAIAATEGVDGIFVGPSDLAAALGHLGNASHPDVQKAIQHIFNRASAHGKPSGILAP
                     VEADARRYLEWGATFVAVGSDLGVFRSATQKLADTFKK"
     gene            2291..3181
                     /gene="garR"
                     /locus_tag="ACHKTW_004928"
     CDS             2291..3181
                     /gene="garR"
                     /locus_tag="ACHKTW_004928"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HFO6465798.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGEIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            3278..4423
                     /gene="garK"
                     /locus_tag="ACHKTW_004929"
     CDS             3278..4423
                     /gene="garK"
                     /locus_tag="ACHKTW_004929"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HFO6465799.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSIPVADG
                     GEGTVEAMIAATQGSERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYAEVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTDDVGVVH
                     QHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNAG"
     gene            4456..4832
                     /gene="rnpB"
                     /locus_tag="ACHKTW_004930"
     ncRNA           4456..4832
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACHKTW_004930"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(5227..>5385)
                     /locus_tag="ACHKTW_004931"
     CDS             complement(5227..>5385)
                     /locus_tag="ACHKTW_004931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6465800.1"
                     /translation="ELLSDNTLEKDENSPDNGFEVGEYNTYEAYNSENQYFTREDYTY
                     NYDLLNAI"
BASE COUNT         1276 a         1277 c         1510 g         1322 t
ORIGIN      
        1 tagcatttgc acaatgatta ttattctcgc gctgcttata ctaattcccg agttcattgc
       61 cgttacggca taaatgaaac tcataaataa caccttcccc ataataataa aaatcagcat
      121 aagtacccga ggtaaataaa atgattctgg acaccgttga cgaaaaaaag aaaggcgtgc
      181 atacccgcta tttaatatta ctgattattt ttattgttac cgccgttaac tacgccgatc
      241 gtgcaacgct gtctattgct ggtaccgaag tggcaaaaga gttgcagtta agtgcggttt
      301 cgatgggtta catcttttct gcttttggct gggcctactt gctgatgcaa atccccggcg
      361 gctggctgct tgataagttt ggctcgaaaa aagtttatac ctatagcctc tttttctggt
      421 cgctattcac tttcctgcaa ggctttgttg atatgttccc gctggcctgg gcagggatct
      481 ccatgttctt tatgcgcttt atgctcggct tctcggaagc gccatcattc ccggcgaacg
      541 cccgtattgt cgccgcctgg ttcccgacga aagaacgtgg tactgcctcc gccatcttta
      601 actcggcgca atatttctcg ctggcgctct tttcgcctct gcttggctgg ctgactttcg
      661 cctggggctg ggagcacgtc tttaccgtta tgggggtgat tggttttgtg ctgacggcgc
      721 tgtggatcaa attgattcat aacccgacag atcacccacg tatgtctgcg gaagagctga
      781 agtttatctc tgaaaatggc gcggtggtcg atatggacca caaaaagccg ggcagtgcgg
      841 cagcaagcgg tcccaaactg cattacatca agcaattgct ctctaaccgc atgatgttgg
      901 gcgtattttt cggacaatat tttatcaaca ccatcacctg gttcttcctc acctggttcc
      961 cgatttatct ggtgcaggaa aaaggcatgt cgattctgaa agtgggtctg gtcgcctcga
     1021 ttccggcact gtgtggtttt gcgggcggcg tgctgggagg tgtcttctcg gattatctga
     1081 tcaaacgcgg tttatccctg accctggcac gtaagctacc gattgtgctg ggaatgttgc
     1141 tggcttccac catcatctta tgtaactaca ccaacaacac cacgctggtg gtcatgctaa
     1201 tggcgctggc tttttttggc aaaggatttg gtgctctggg ctggccggtg atttctgaca
     1261 ccgcgccgaa agagattgtt ggcctctgtg gcggcgtctt taacgtcttt ggcaatgttg
     1321 cctccattgt cactccactg gtgattggct acctggtaag tgaactgcac tccttcaatg
     1381 cagcactggt tttcgtggga tgttcagcgc tgatggcgat ggtctgctac ctcttcgtag
     1441 ttggcgacat taaacgtatg gaattgcaga aataagcaaa ggtataagcg atgaataacg
     1501 atgttttccc gaataaattc aaagccgcac tggctgcgaa acaggtacaa attggttgct
     1561 ggtcagcact ctctaacccg attagcactg aagttcttgg tttggctggg tttgactggc
     1621 tggtgctgga tggcgaacat gcgccaaacg atatctccac gtttattccg cagttaatgg
     1681 ccctgaaagg cagcgccagc gcgccagtag tgcgagtgcc gaccaacgag ccggtaatta
     1741 ttaagcgtct tctggatatc ggtttctata acttcctgat tccttttgta gaaacaaaag
     1801 aggaagcaga gcaggcggtg gcatcaaccc gttacccacc ggaaggcatt cgcggcgtct
     1861 ccgtttctca ccgcgccaat atgtttggca ccgtggcgga ttatttcgct cagtcgaaca
     1921 agaacatcac cattctggtc cagatagaaa gtcagcaggg cgtagataac gtcgatgcca
     1981 ttgccgctac cgaaggtgta gacggtatct tcgtcggccc cagcgatctg gctgcggcat
     2041 taggccatct cggcaatgcc tcgcacccgg atgtacaaaa agcaattcag cacattttta
     2101 accgtgccag tgcgcacggc aaacccagcg gtattctcgc gccggtcgaa gccgatgcgc
     2161 gtcgttatct ggaatggggc gcgacgtttg tggctgtcgg cagcgatctt ggcgtcttcc
     2221 gctctgccac tcagaaactg gctgatacct ttaaaaaata accaccaccg caacaagaga
     2281 gatgattgat atgactatga aagttggttt tattggcctg gggattatgg gtaaaccaat
     2341 gagtaaaaac cttctgaaag caggttactc gctggtggtt gctgaccgta acccagaagc
     2401 tattgctgac gtgattgctg caggtgcaga aacagcgtct acggctaaag cgatcgctga
     2461 acagtgcgac gtcatcataa ccatgctgcc aaactcccct catgtgaaag aggtggcgct
     2521 gggtgagaat ggcatcattg aaggcgcgaa gccaggtacg gtattgatcg atatgagttc
     2581 tatcgcaccg ctggcaagcc gtgaaatcag cgaagcgctg aaagcgaaag gcattgatat
     2641 gctggatgct ccggtgagcg gcggtgaacc gaaagccatc gacggtacgc tgtcagtgat
     2701 ggtgggcggc gacaaggcta ttttcgacaa atactatgat ttgatgaaag cgatggcggg
     2761 ttccgtggtg cataccgggg aaatcggtgc aggtaacgtc accaaactgg caaatcaggt
     2821 cattgtggcg ctgaatattg ccgcgatgtc agaagcgcta acgctggcaa ccaaagcggg
     2881 cgttaacccg gacctggtgt atcaggcaat tcgcggtgga ctggcgggca gtaccgtgct
     2941 ggatgccaaa gcgccgatgg tgatggaccg caacttcaag ccgggcttcc gtattgatct
     3001 gcatattaag gatctggcaa atgcgctgga tacttcccac ggcgtcggcg cgcaactgcc
     3061 gctcacagct gcggttatgg agatgatgca ggcactgcga gcagatggtt taggaacggc
     3121 ggatcatagc gccctggcgt gctactacga aaaactggcg aaagtcgaag ttactcgtta
     3181 atgacgtcgt gcccggatgg cgtattgcaa tccgggcctg gaatccaggc cgaataagag
     3241 aaacgccctc cggcgtcatg tggtaacagg cataggtatg aaaatcgtaa tcgccccaga
     3301 ctcttataaa gaaagtttat ctgccagcga ggttgcgcag gcgatagaaa aaggatttcg
     3361 ggaaattttt cctgatgcac agtacgtttc tattccggtt gccgacggtg gcgaaggaac
     3421 ggtggaagcg atgattgcag ccacccaggg ttctgaacgt cacgcctggg ttacagggcc
     3481 gctgggcgag aaagtgaatg ccagttgggg gatctccggc gatggcaaaa ccgcgtttat
     3541 tgaaatggcg gcggccagtg ggctggagct ggtacctgcg gaaaaacgcg atccactcgt
     3601 gaccacttca cgcggcacag gcgagttaat cctgcaggcg ctggagagcg gcgcgacaaa
     3661 cattattatc ggcattggcg gtagcgctac aaatgatggc ggcgcaggca tggtacaggc
     3721 gctgggggcg aaattatgcg acgccaacgg caatgaaatt ggttttggcg gcggtagtct
     3781 taatactctg aatgatattg atatttccgg cctcgatccg cgcttaaaag attgcgtcat
     3841 tcgcgtcgct tgtgatgtca ccaatccgct ggtgggcgat aacggcgcat cgcgcatctt
     3901 tggcccacaa aagggagcca gtgaagcgat gattgttgag ctggacaata acctctctca
     3961 ctatgccgag gtcattaaaa aagcgctgca tgttgatgtg aaagatgtcc ccggcgcagg
     4021 agctgcgggc ggtatgggtg cggcgctgat ggcgtttctt ggtgcggaac tgaaaagtgg
     4081 tattgaaatc gtcactacgg cgctgaatct ggaggaacat attcacgatt gtacgctggt
     4141 gatcaccggt gaagggcgta ttgacagcca gagtattcac ggcaaggtac cgattggcgt
     4201 tgcaaacgtg gcgaagaagt accataaacc ggtgattggc attgcgggta gcctgaccga
     4261 tgatgttggc gttgtacatc agcatggcat cgatgcggtc ttcagcgtat tgaccagcat
     4321 aggtacgttg gacgaagcat tccgcggggc ttatgacaat atctgccgtg cttcacgtaa
     4381 tatcgccgcg acactggcga ttggaatgcg caacgcgggg tgacaagggc gcgcaaaccc
     4441 tctatactgc gcgccgaagc tgaccagaca gtcgccgctt cgtcgtcgtc ctcttcgggg
     4501 gagacgggcg gaggggagga aagtccgggc tccatagggc agggtgccag gtaacgcctg
     4561 ggggggaaac ccacgaccag tgcaacagag agcaaaccgc cgatggcccg cgcaagcggg
     4621 atcaggtaag ggtgaaaggg tgcggtaaga gcgcaccgcg cggctggtaa cagtccgtgg
     4681 cacggtaaac tccacccgga gcaaggccaa ataggggttc ataaggtacg gcccgtactg
     4741 aacccgggta ggctgcttga gccagtgagc gattgctggc ctagatgaat gactgtccac
     4801 gacagaaccc ggcttatcgg tcagtttcac ctgatttaac gaaaacccgc cagcgtaaac
     4861 cggcgggttt ttgcttttgg aggggcagaa agatgaatga ctgtccacga cgctataccc
     4921 aaaagaaagc ggcttatcgg tcagtttcac ctgatgtacg taataaaccg ttccggcggg
     4981 tttcagattg ttgagtgcgc tttattcatg ccggatgcgg cgtgaacgcc ttatcctgcc
     5041 tacaaaagca cgcaaattca atatattgca gagatggcgt aggcctgata agcgtagcgc
     5101 atcaggcatt tttgcggttg ccatcagttt caaacccgcg tcagtgagtt tttgcttttg
     5161 gtgcacaggg taagatgaat aaatatctac ggtattaata atagactttg taagaagtta
     5221 ttaagtctat attgcattta aaaggtcgta attatacgta tagtcctctc tggtaaaata
     5281 ttgattctct gagttatatg cttcatatgt gttatattcc ccgacctcaa aaccattgtc
     5341 cggagaattt tcgtctttct cgagggtgtt atctgaaagt aactc
//
LOCUS       DAZFQD010000220         5296 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.220, whole genome shotgun sequence.
ACCESSION   DAZFQD010000220 DAZFQD010000000
VERSION     DAZFQD010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5296
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.220"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            127..1485
                     /gene="mltD"
                     /locus_tag="ACHKTW_004932"
     CDS             127..1485
                     /gene="mltD"
                     /locus_tag="ACHKTW_004932"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HFO6465801.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDGDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAD
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMP
                     DS"
     gene            complement(1533..2303)
                     /locus_tag="ACHKTW_004933"
     CDS             complement(1533..2303)
                     /locus_tag="ACHKTW_004933"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFO6465802.1"
                     /translation="MTTQSHHDHVEKQFSSQASEYLTSTVHASGRDLQRLAVRLADYP
                     DASVLDMGCGAGHASFVAAQNVSAVVAYDLSAQMLDVVAQAAEARQLKNITTRQGYAE
                     SLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGRMIVMDVMSPGHPVRDIWL
                     QTVEALRDTSHVRNYASGEWLRLINEANLIVDNLITDKLPLEFSSWVARMRTPEALVD
                     AIRIYQQSASTEVRTYFALQNDGSFTSDIIMVEAHKAA"
     gene            complement(2381..3181)
                     /locus_tag="ACHKTW_004934"
     CDS             complement(2381..3181)
                     /locus_tag="ACHKTW_004934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFO6465803.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGESLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            3422..4336
                     /gene="yafC"
                     /locus_tag="ACHKTW_004935"
     CDS             3422..4336
                     /gene="yafC"
                     /locus_tag="ACHKTW_004935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YafC"
                     /protein_id="HFO6465804.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMASAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHVCLGFTEPASLNTWPIACSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDKEIARGELVELMADKVLPVAMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAPGGAVREA"
     gene            complement(4333..5136)
                     /gene="dkgB"
                     /locus_tag="ACHKTW_004936"
     CDS             complement(4333..5136)
                     /gene="dkgB"
                     /locus_tag="ACHKTW_004936"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HFO6465805.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKQGLTREIGISNFTIPLMEKAIAAVGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNATPAQVILAWAMGEGYSV
                     IPSSTKRKNLESNLKAQNLQLDAKDKKAIAALDCNDRLVSPKGLAPEWD"
BASE COUNT         1388 a         1276 c         1316 g         1316 t
ORIGIN      
        1 gcaacaacct gaagaacgtt ttgcatggtt aaggtcaaag aaagataggt tctgataaaa
       61 ctttcttgtc atcagctccg ttcgccgtta tgatcggtcg tcttttaagc aactattgac
      121 acacacatga aggcaaaagc gatattactc gcctctgtcc tgctcgtggg ttgccagagt
      181 accggcaacg ttcaacagca cgcacagagc ctttctgcag ctggtcaagg ggaagcagca
      241 aagtttacaa gtcaggcacg atggatggac gatgggacgt ctatcgcgcc agatggtgac
      301 ttgtgggctt tcattggcga cgagctaaag atgggaattc cggaaaatga ccggattcgc
      361 gaacagaaac agaaatattt acgcaataag agctatctcc acgatgtaac tttacgggca
      421 gagccgtata tgtactggat agccgggcaa gttaaaaaac gtaacatgcc tatggaactg
      481 gtactactac ccatagtgga gagcgctttt gatcctcacg caacgtctgg cgccaatgcc
      541 gcaggcatct ggcagatcat tccgagcacg gggcgcaatt atggtttgaa acagacccgc
      601 aattatgacg cgcgtcgcga tgttgttgct tcaacaactg ccgcgctgaa catgatgcag
      661 cgtctgaaca agatgtttga cggcgactgg cttctgaccg tagcggctta taacagcggc
      721 gaaggtcggg tcatgaaggc aattaaaacg aacaaagcgc gtgggaaatc cacggacttc
      781 tggtcgttac cgttgccgca ggaaacgaag cagtacgtgc ctaaaatgct ggcattgagt
      841 gatattctca aaaacagcaa gcgttatggc gtacgtctgc caacgaccga tgaaagccgt
      901 gctctggcgc gtgtgcacct gagcagcccg gttgaaatgg cgaaggttgc agatatggcg
      961 gggatttccg tcagcaagct gaagacattc aacgctggcg tgaaaggctc cacgctgggc
     1021 gcaagtggtc cgcaatacgt gatggtgcca aagaagcatg cagatcaact gcgtgaatct
     1081 ctcgcttcag gcgaaattgc tgctgtacag tcgacgctgg ttgccgacaa tacgccgctt
     1141 aacagccgtg tttacaccgt acgctctggc gacacgcttt caagtatcgc ttcacgtctc
     1201 ggcgtaagca ccaaagattt gcagcagtgg aacaaactgc gcggatctaa gctgaagcca
     1261 ggccaaagtt tgacgattgg tgcaggcagt agcgcacagc gactggcaaa caacagcgat
     1321 agcattacgt atcgtgtgcg caaaggcgat tcgctttcaa gcattgctaa acgccacggc
     1381 gtgaacatca aagatgtgat gcgctggaac agcgacactg cgaatctgca accaggcgac
     1441 aagctgacgt tgtttgtgaa aaacaacaac atgccagatt cctgacaaac cagataataa
     1501 aaaggcaccg attcccccgg tgcctttttt atttatgctg ctttatgtgc ttctaccatg
     1561 atgatatcac tggtgaaaga gccatcattc tgcaaggcaa aatacgttct cacctctgtc
     1621 gatgcgctct gttggtaaat gcgaatagcg tctactaacg cttctggcgt acgcattctc
     1681 gcgacccatg aagaaaattc cagcggtaac ttatctgtaa ttaaattatc aactatcaga
     1741 ttggcttcat tgattaacct caaccactca ccgctggcgt agtttcgtac gtgagaggta
     1801 tcgcgtaatg cttctaccgt ctgtaaccag atgtcgcgca ctggatgacc cggagacatt
     1861 acgtccatca caatcatcct accgccaggt ttcaatatcc tattcacttc tcgcagtgct
     1921 gcaccaacat catgccagtg atgggcagaa taacggctga taacaatatc aaatgcgtta
     1981 tcggcaaatg gcagactttc ggcataaccc tggcgggtgg tgatattttt cagttgccgg
     2041 gcttcggcag cttgtgccac gacatccagc atttgggcag ataagtcata cgccaccacc
     2101 gcgctcacgt tctgcgcagc gacaaagctg gcatgccctg ctccgcagcc catatcaagt
     2161 acacttgcat caggataatc agccagacgc accgccaggc gctgtaaatc tcgcccggat
     2221 gcatgtacgg tactggttaa atactcactg gcctgcgagc taaactgctt ttctacgtgg
     2281 tcatggtggg attgtgttgt cattgcgctg tccttttgtt gtttgagtat aaaaggacag
     2341 cgccaaacgt tggtctgccc tgtggcagac ctgacatacc ttatttatca ggcttgccgg
     2401 gactgaattc aacgagtagc ggattgtgat cggaagcgcg cgtaaccaat acagaagctt
     2461 cgctgacgtt cagaccacgg tagaaaacaa aatcgagcgg gcggccaaac gcccgacggc
     2521 gctgatcatc ggtaaaacgc acctggcgca gcgacatttc ccgcgcaaag cgatataacg
     2581 cgttcatcct tctacggctc caggcattga aatctcctgc cataatgacc gggccgctgt
     2641 ggtgagctat ctgatcacca ataggaagta actgcttact atagacatcc acgcccagac
     2701 tgaagttgac ggcgtgtata ttaaccacca tcaacaggcg ggtgtcaggt aatggataga
     2761 ccgtcaccag tgccgacttc gccagacgca aaatgggttc tcgttcacgt aacgggcagc
     2821 aatacactgg atgcgccgcc gaaagggtca ttacgccaga aggatgttgt ggcagtacga
     2881 aagcgggtac ctgatcggcg gcaagatagt tagcggtcgc aaactgtact aactctggcg
     2941 ttgtctgcgc ttcctgcaat aacaccagat gagcatcttt gccgtagttc tttaataccg
     3001 acaaccattc agcgcgttgc tgtttgtata tgttccacac caggatccga atacgctctt
     3061 cggtacttaa cgattctccg ggtggtaatg cctggccgat gctcgcaaaa gaccccggcg
     3121 gtaagatcct ttccgcaggt tgtccggcaa catagcgcat ggcataggtg ttttttcgca
     3181 cttttgactt tgaaacctct attacgtcaa cccttccgca ctcgggaggg ttctactgtt
     3241 atagggattt tagctcactc tttcaacgag caattgtata ttgttatgta agcaagtgct
     3301 tacaccggtc agaattttgc tcattatgga gaacattaaa tcgcatgaca tatcacgcat
     3361 cctgatggtt taatgacaaa taaattcaaa atatctcttc tattttgcaa caggagcaaa
     3421 aatgaaagcc acgtcggaag aactcgccat ttttgtttcg gtcgtcgaaa gcggcagctt
     3481 tagccgggca gcggaacaat tagggcaagc aaactcagcg gtaagccggg cggtgaaaaa
     3541 gctggagatg aaacttggcg ttagcctgct taatcggacc acgcgacaac ttagcctgac
     3601 ggaagaaggc gagcgttatt ttcgtcgcgt acagtcaatt ttgcaggaga tggcatcggc
     3661 agaatcagaa attatggaga cgcgtaatac accgcgtgga ctgttacgga tcgatgccgc
     3721 aactccagtg gtgctgcact ttctgatgcc gttaattaag cctttccgtg aacgctatcc
     3781 ggaagtcact ttgtcgctag tctcctccga aacgattatt aatttgatcg aaagaaaagt
     3841 ggatgtcgcg atacgcgctg gtacgttaac ggattccagc ttacgtgcca ggccgttatt
     3901 taacagttat cgaaaaatta tcgcctcccc cgattatatt tcccgctacg ggaagccaga
     3961 aacgatcgac gatttaaagc aacatgtttg cctgggattc actgaacccg cttccctcaa
     4021 tacctggccg atagcctgta gtgatggaca attacatgag gtgaagtacg gtttgtcatc
     4081 caatagtggg gaaacactga aacagctttg cctgagtggc aacgggattg cgtgtttgtc
     4141 cgactacatg atcgacaaag aaatcgctcg cggagaattg gtggagttaa tggcagataa
     4201 agtgttgcca gtggcaatgc cattcagtgc agtctattac agcgaccgtg cggtaagtac
     4261 gcgcatccgg gcttttatcg atttccttag cgagcatgta aaaacagctc ccggaggagc
     4321 tgtcagagag gcttaatccc attcaggagc cagacctttc gggctaacca ggcggtcgtt
     4381 gcaatccagg gcggcgatcg cttttttatc tttggcatcg agctgtaaat tttgtgcctt
     4441 aagattactt tccaggtttt tacgtttagt agaagaagga attactgagt aaccttcccc
     4501 catagcccac gccagaatca cttgtgccgg agtcgcattg tgtttagctg cgatacgagc
     4561 aataacctca tctttcaggg ccttaccata cgccagcgtc atataggaag taatatggat
     4621 gccgtgctgt ttagcccagg caaccacttt acggttttgc agataaggag agagttcaat
     4681 ctggttagta gcgatgtttt cagcgccaac agcagcaatc gccttttcca tcaatgggat
     4741 cgtgaagttg gaaataccga tctcacgcgt cagcccttgt tttttggctt ccagcagcgc
     4801 ctgcataaac tcttcaacag agacttcatc gtttggtgac ggccagtgga ttagcgtcag
     4861 atcaacataa tcggtacgca atttttgcag gctctctttc agacttggga tcaatttgtc
     4921 tttgctgaga ttttcaatcc agattttagt ggtgatgtag agttcatgac gtggcacgcc
     4981 actttctgca atcgcctgac ctactgcggc ttcgttatca tagatttgtg cggtatcaat
     5041 tgcgcgataa ccaagttcaa gcgccgtttt cacagatgaa ataacaacgt cgtctttcag
     5101 acggaaagta cctaaaccaa atgcagggat agccataata tgcctctttt aatgctattt
     5161 tcgttaactg aggtgattat gggcttcaga ctgttgagga aaaagagcgt aaaatgcaga
     5221 ggatttttgc gattctggca ataatagata tacagacgaa aaaaaacctc gagcgttaac
     5281 tcgaggcttc ttaata
//
LOCUS       DAZFQD010000221         5114 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.221, whole genome shotgun sequence.
ACCESSION   DAZFQD010000221 DAZFQD010000000
VERSION     DAZFQD010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5114
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.221"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..305)
                     /locus_tag="ACHKTW_004937"
     CDS             complement(<1..305)
                     /locus_tag="ACHKTW_004937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000062365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO6465806.1"
                     /translation="MSMNLMAKAMNIKVGNPLRKLVLIKLADNANDNGECWPSYQHVA
                     DQCEVSRSTVKSHIRALEEMGLLKRECRRKGELNQSNVFYLTLDNAQQIPPESGGAGA
                     "
     gene            complement(468..764)
                     /locus_tag="ACHKTW_004938"
     CDS             complement(468..764)
                     /locus_tag="ACHKTW_004938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CII family transcriptional regulator"
                     /protein_id="HFO6465807.1"
                     /translation="MTQTSYSKLSQRDVDRAETDLLINLSTLTQRGLAKMIGCHESKI
                     SRTDWRFIASVLCAFGMASDISPISRAFKYALDGITNKKRPAATERSDQIQMEF"
     gene            complement(903..1130)
                     /locus_tag="ACHKTW_004939"
     CDS             complement(903..1130)
                     /locus_tag="ACHKTW_004939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO6465808.1"
                     /translation="MQNLDEPIKGVGIPEVAKACGVSERAVYKWLKNGFLPKTEFFGK
                     TKYASKIEEISGGKYQASEMLEISKKNLLAA"
     gene            1209..1916
                     /locus_tag="ACHKTW_004940"
     CDS             1209..1916
                     /locus_tag="ACHKTW_004940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000250468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HFO6465809.1"
                     /translation="MVQNEKVRKEFAQRLAQACKEAGLDEHGRGMAIARALSLSSKGV
                     SKWFNAESLPRQEKMNALAKFLNVDVVWLQHGTSLNGANDEDTLSFVGKLKKGLVRVV
                     GEAILGVDGAIEMTEERDGWLKIYSDDPDAFGLRVKGDSMWPRIKSGEYVLIEPNTKV
                     FPGDEVFVRTVEGHNMIKVLGYDRDGEYQFTSINQDHRPITLPYHQVAKVEYVAGILK
                     QSRHLDDIEAREWLKSS"
     gene            1997..3022
                     /locus_tag="ACHKTW_004941"
     CDS             1997..3022
                     /locus_tag="ACHKTW_004941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6465810.1"
                     /translation="MKDIINVSSLDDIESLFARLDGGEEITIDRIKLDLFNNVNFKIY
                     GDPSRYNGTLPSPLAQGLCEFQTEIYKVFTLIKYKTDNLQRLTGKDKEEAEIIFTINE
                     GCTDILAALTELFEAFGKAFEKVTHGMSPSQKTLCFLFAVTVIGGAWVGTSYLEKKAE
                     VEVKQEETKLEEAKIKSENERMTILRDGMLEAIRAKAGIDAVERAEGIQEHTAKAYTG
                     VLKGASDADKIVISGASTVELSNKEIHELIKNPIEKAKKEERNLELVIDGIKRTAEKI
                     TLSCREPSSEDSFPVSVDTSFIDDKDEIAILFDAMKENRTVKILGSYTIRAGVIENGN
                     ASTITRP"
     gene            3804..3872
                     /locus_tag="ACHKTW_004942"
                     /pseudo
     CDS             3804..3872
                     /locus_tag="ACHKTW_004942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311021.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
     gene            3934..4557
                     /locus_tag="ACHKTW_004943"
     CDS             3934..4557
                     /locus_tag="ACHKTW_004943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001597931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nmad5 family putative nucleotide modification
                     protein"
                     /protein_id="HFO6465811.1"
                     /translation="MSSIRLTTRMKEEIARNALIKSGVFTELEEVTKLKNQLALDARV
                     IAFGGKKKTEEVDQLASKLMAASEELQKLGCSFYSCDVSSCSIYLTVSGRRVGWHSYG
                     KDGNGEDILLPTPDKDKCMFDAEHKITKRFDEICALQQKLEARKKDIESNVWAALNSV
                     TTVKRLIEVWPESKELLPKEADKASAALPALRVEDLNKMIGLPSEAA"
     gene            4782..4922
                     /locus_tag="ACHKTW_004944"
     CDS             4782..4922
                     /locus_tag="ACHKTW_004944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001200014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease FtsH-inhibitory lysogeny factor CIII"
                     /protein_id="HFO6465812.1"
                     /translation="MQYAIAGWPVAGCPSESLLERITRKLRDGWKRLIDILNQPGVPK
                     NG"
     gene            4915..5058
                     /gene="kil"
                     /locus_tag="ACHKTW_004945"
     CDS             4915..5058
                     /gene="kil"
                     /locus_tag="ACHKTW_004945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitory peptide Kil"
                     /protein_id="HFO6465813.1"
                     /translation="MDKTLMAIQTKFTIATFIGDEKMFREAVDAYKKWILILKLRSSK
                     SIH"
BASE COUNT         1534 a         1060 c         1137 g         1383 t
ORIGIN      
        1 gctcctgccc cacctgattc tggtgggatt tgttgtgcat tatccagcgt cagataaaaa
       61 acgtttgact ggttaagctc tccttttctt ctgcattccc ttttcaaaag acccatctct
      121 tccagtgccc taatgtgact ttttactgtc gatctgctca cctcacactg gtcagcgaca
      181 tgttgatatg aaggccagca ttcgccatta tcattggcgt tatcggcaag tttaatcaga
      241 accagttttc tcagtgggtt gccaaccttt atattcatgg ccttagccat aagattcatg
      301 ctcattttga cttctccgaa gttttgtacc tgttaagtat ttctctcagt ggcacagcta
      361 ttgctggatt aactccctga taaaactggt cacgtagcac atcttttcgg tgattaacgc
      421 gtttattttc ctgcgttttt cgcatataat tacctcgttg gatgttgtta aaattccatt
      481 tgtatttgat cagaacgctc ggttgccgcc gggcgttttt tattggtgat tccatcaagc
      541 gcatacttaa aagccctgct aatcggactg atgtctgatg ccattccgaa agcacacagg
      601 accgaagcaa taaatctcca gtccgttctg cttatcttcg attcatgaca gccaatcatc
      661 tttgccagac cgcgctgggt aagcgttgac aggttgatga gtaaatccgt ttctgcgcga
      721 tcaacgtcgc gctgggatag tttgctgtaa cttgtttgtg tcatttctta atatttccaa
      781 tagtgaatag ttagttgaaa ggtatgcgtg gaaacgcata tggccttagt tggtcagata
      841 tcttggggct cgctttgtca gcgacgtagg acgaatgtcc attgtgaaaa tagcggtgtt
      901 acttatgcag ccagaaggtt ctttttgctt atttcaagca tttcgcttgc ttgatatttg
      961 ccaccagaaa tctcttcgat ttttgatgcg tatttcgttt tcccaaaaaa ctcagtctta
     1021 gggaggaagc cgtttttgag ccacttatag acagcccttt cgctaactcc acaagccttc
     1081 gcaacttcag ggatgccgac acctttaatc ggctcatcaa gattttgcat agggatatcc
     1141 tttttcgtac tttcagtacg cattatgatt gaactgaaag tttttgcaag tgctttagta
     1201 tcgtactcat ggttcagaat gaaaaagtgc gcaaagaatt cgcccagcgg ctagcgcaag
     1261 cctgtaaaga agctggtctt gatgaacatg gtaggggaat ggctatagcc cgtgcccttt
     1321 ctctttcgtc caaaggcgtt agcaaatggt ttaatgctga gtctttaccg cgtcaggaaa
     1381 aaatgaatgc gcttgcgaaa tttctaaacg ttgatgttgt ttggcttcag cacggcactt
     1441 cgttaaatgg agcgaatgat gaagatactc tttcatttgt tggcaaatta aaaaaagggt
     1501 tagtgcgcgt ggttggtgag gcaattcttg gtgttgatgg tgccatcgag atgaccgaag
     1561 agcgcgatgg gtggctcaaa atttatagcg atgatccaga tgcctttggc cttcgtgtga
     1621 aaggagacag catgtggccc agaataaaat caggagaata tgtactcatt gagcctaaca
     1681 ccaaagtatt cccgggtgat gaggtgtttg tcagaaccgt tgaaggacac aacatgatta
     1741 aggttcttgg ctatgacaga gatggagaat accaatttac aagcatcaac caagaccaca
     1801 ggccaataac gttgccttat catcaagtag caaaggtgga gtatgtggct ggtattctga
     1861 agcaatctcg ccatctggat gacatcgagg caagggagtg gctgaaaagt tcgtgacttc
     1921 atcgtcacat agctggtaag tatccaattt ttataatcac taatttaact agcaaggttg
     1981 tgccgaaggt gttgtaatga aagatattat taatgtttca agtctggacg acattgaatc
     2041 tttgtttgca aggttagatg gtggcgaaga gatcactatc gacaggatca agttagacct
     2101 cttcaataat gtgaatttta agatttatgg tgatccttct cgctacaacg gaacgcttcc
     2161 atctcctcta gctcagggtt tatgcgaatt tcagactgaa atttataaag ttttcacact
     2221 aataaaatat aaaactgaca accttcaaag gttaactggt aaagataaag aggaagcaga
     2281 gatcatcttt accattaatg aaggttgtac tgatatactt gctgcattaa cagagctttt
     2341 tgaagcattc ggtaaagcct ttgagaaggt aacacacggt atgagtccta gccaaaaaac
     2401 attatgcttc ttgtttgctg tcaccgttat aggcggtgca tgggttggta catcatactt
     2461 agagaaaaaa gccgaagtag aagtcaagca agaagaaaca aagctcgaag aagccaagat
     2521 taaatcagaa aacgagcgca tgacgatatt aagggacgga atgcttgagg cgattagagc
     2581 taaagcgggc atagatgcag tagagcgcgc cgaagggatt caagagcata ctgcaaaagc
     2641 gtatacaggg gtattaaaag gcgcgtctga tgctgacaaa attgtcatat caggcgcaag
     2701 cacagttgaa ctgtctaaca aagaaatcca cgaactaatc aagaacccta tcgaaaaagc
     2761 aaaaaaagaa gaaagaaacc ttgaattagt cattgatgga atcaagcgta ctgcggaaaa
     2821 gataacccta agctgccgcg aaccatctag tgaagacagt ttccctgttt cggtggacac
     2881 gtcgttcata gatgataaag acgagatcgc catactgttt gatgcaatga aagaaaacag
     2941 gacagtaaaa atcttgggaa gctacacgat acgagcaggt gtaatagaaa acggaaacgc
     3001 atcaaccatc actcgcccgt aacagaaaat ctaaccccgg ccaccgagcc gggttttatt
     3061 tgcctctcga tcccccacct aaaaacacat aaccaattgt atttattgat gtaactcgct
     3121 aaaccatgca gttctgatcc ctgccgcata accttcatca gccacatttt caaaaataaa
     3181 tttccttata tatcagaatc atacttcgta gagttaataa atcaccaaaa ttcgtaccaa
     3241 tagttcttga taatgtcgaa ctattggttc attattatcg tcatcagcag gacgcactac
     3301 tcaccagggc ggtgaatata caacgattcg aatatgaatc tacggcgctg acaaagcgca
     3361 ataaccaaag tgaactttgg ggtgaatgca gaagctaacc ttctcggcgg aggcgctttg
     3421 caatgattac gcgaccggag ttagtcgccc ggctgtattc accgccaaag ttcatcagga
     3481 ggttccatat gacacgcaga actcagttca aaggcaattc acgttctcgt cgtcgtgagc
     3541 gtttaaaggc aaaggcatta gctaacggcg tgctggcccg cgaagaagca ataagttcag
     3601 aagtattaca ccgccctact ctaagcagag cgcagattca ggctaaaggt actcacgaaa
     3661 cgcctgagcg catagaagac gctaagccaa ttaagttcat ggcacaggac gtgatctggc
     3721 aacagaaaga atacagacgc aatctggagc gagcggccat tgtgtacgcg aatgagtttg
     3781 gacataagca accagaaact ggtgtatgtc ttccaaacgt agccatttac gctgcaggct
     3841 accgtaaatc caaacaactg acagcaagat gacttgtgtt ggtcgccaga aaatgaaatt
     3901 aggcagcaaa ccacttattt gagaggaatt aatatgtcat caatccgctt aactacgaga
     3961 atgaaagagg aaatcgctcg taacgcttta attaagtccg gggtgttcac tgaacttgaa
     4021 gaagtaacaa agttaaagaa ccagcttgca cttgacgcca gagttattgc gtttggcggg
     4081 aaaaagaaaa ccgaagaagt ggaccagctg gcatcaaagt taatggctgc aagtgaggaa
     4141 cttcaaaagc tgggatgttc attttactca tgcgatgtca gttcatgttc gatttatctg
     4201 actgtatctg gaagaagggt tggctggcat tcatacggaa aagacggcaa cggtgaagat
     4261 atattgctcc ctacccccga caaagataaa tgcatgtttg acgcagaaca caaaataaca
     4321 aaaaggtttg atgaaatctg cgcattgcaa caaaaacttg aagccaggaa aaaggatata
     4381 gaatcaaacg tatgggctgc tttgaactca gtcacaacag ttaagcgact tattgaagtt
     4441 tggcctgaaa gcaaagagtt gctaccaaaa gaagcagata aagcaagtgc agcacttcct
     4501 gctttacggg tagaagattt gaataagatg attggacttc cttccgaggc cgcataatcg
     4561 gcctttattt ttggcataaa caacagaata aacactgcac tgtgtattca ttccaacgag
     4621 tgaatacacg gagcaatgtc gctcgtaact aaacaggagc cgacttgttc tgattattgg
     4681 aaatcttctt tgccctccaa tgtgagggct tttttatatg cataccaata acgcttcact
     4741 agaggcgttt tcgttatgtg taaataaata aggagcacac catgcaatat gccattgcag
     4801 ggtggcctgt tgctggctgc ccttccgaat ctttacttga acgaatcacc cgtaaattac
     4861 gtgacggatg gaaacgcctt atcgacatac ttaatcagcc aggagttccc aaaaatggat
     4921 aaaacactta tggctatcca gactaaattc actatcgcca cttttattgg tgatgaaaag
     4981 atgtttcgtg aagccgtcga cgcttataaa aaatggatat taatactgaa actgagatca
     5041 agcaaaagca ttcactaacc cctttcctgt tttcctaatc agcccggcat ttcgcgggcg
     5101 atattttcac agct
//
LOCUS       DAZFQD010000222         5055 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.222, whole genome shotgun sequence.
ACCESSION   DAZFQD010000222 DAZFQD010000000
VERSION     DAZFQD010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5055
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.222"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            6..869
                     /gene="ypfJ"
                     /locus_tag="ACHKTW_004946"
     CDS             6..869
                     /gene="ypfJ"
                     /locus_tag="ACHKTW_004946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HFO6465814.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHSMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            884..2899
                     /gene="tmcA"
                     /locus_tag="ACHKTW_004947"
     CDS             884..2899
                     /gene="tmcA"
                     /locus_tag="ACHKTW_004947"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HFO6465815.1"
                     /translation="MAELTALYTLTAQMKREGIRRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQPDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFDDENFTHTPQGNIVISAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQYFLQAAGENEIAGALWLVDEGGLSQQLSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGTGQQLIAGALQYTRDLDYLSVSFGYTGELWRFWHRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNGETLPVD
                     PLNDIVLSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNASDAQ
                     LCTTLKLAGRKMLLVRQREEVAHALFALNDVRTERLRDRITQWQFFH"
     gene            2973..3554
                     /gene="ypfH"
                     /locus_tag="ACHKTW_004948"
                     /pseudo
     CDS             2973..3554
                     /gene="ypfH"
                     /locus_tag="ACHKTW_004948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708312.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
     gene            3707..>5055
                     /locus_tag="ACHKTW_004949"
     CDS             3707..>5055
                     /locus_tag="ACHKTW_004949"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO6465816.1"
                     /translation="MTSGKTNLFRFTKSAINQIPPSPKGKQLEYLDTQVKGLRLRVGS
                     TGKKTFVIVRNGKEGFIRVKVGNFPDMSVETARAVALKKLGDLSLTRKNPNKEKKAAE
                     ESNEETNEQRNVTLREALNQYLDLRGERISETTAKQYTSLLSNFSRDWFDLPLAKISR
                     RKVTTRHKQITDGSVWFGSPEQKNKKNVAKGSKAQADLWGRVLRAVYRFAYDYYRDDD
                     EQRLLPEPPTQALTSQRQWNGSKRKTTRIRNHEIGRWYRAVNAVLCDAEAISDFTAMS
                     VCDALISSLFTGMRKGEVLGLTWNNVDLEGGYIRLQDTKNKRDTEIPITQTMREIITR
                     RDKWRIYASDYVYPSFCGEKIENPRRTIIQIMDKTTIIDGEYKAPVKFCFHDLRRTFA
                     SIAELSGVGIYTIKKLMNHKSGQGADVTIGYINMTADELLQPSQIIEKRILKEIGIA"
BASE COUNT         1251 a         1186 c         1432 g         1186 t
ORIGIN      
        1 tatctatgcg ttggcaaggg cgacgtgaaa gtgacaatgt tgaagacagg cgcaacagct
       61 ctggcggtcc gtcaatgggc ggtcccggtt tccgtctgcc gagcggtaaa ggcgggctga
      121 ttttactgat agtcgtgctg gttgcaggct actatggcgt tgatttaacc gggttgatga
      181 ccgggcagcc ggtttctcaa caacaatcaa cgcggtcaat tagccctaat gaagacgaag
      241 ccgcaaaatt cacctcggtg attctggcaa ccacggaaga cacctgggga caacagttcg
      301 agaagatggg taagacctat cagcaaccga agctggtcat gtaccgtgga atgacgcgca
      361 ccggctgcgg ggctggtcag tccattatgg ggccgttcta ttgcccggcg gatggcacag
      421 tttatatcga tctctccttc tatgatgaca tgaaagacaa acttggcgcg gatggcgatt
      481 ttgcccaggg gtacgttatc gcccatgaag tcggtcatca tgtgcagaaa ctgttaggca
      541 tcgagccgaa agttcgtcaa ctgcaacaaa acgcgacgca ggcggaagta aaccgcttat
      601 ctgtgcgtat ggaactccag gccgactgtt ttgccggtgt ctgggggcat agtatgcagc
      661 agcaaggcgt tctggaaact ggcgatctgg aagaggcgct gaatgcggcg caggccatcg
      721 gcgatgaccg tttacaacag caaagtcagg ggcgagtagt accagacagt ttcacacatg
      781 gcacttctca gcaacgctac agctggttta aacgtggttt cgacagcggc gatccggcac
      841 aatgcaatac ttttggtaaa agcatttaat ttccggggca gggatggctg aactgactgc
      901 gctttacaca ttaacagcgc aaatgaaacg tgaagggatc cgccgcttgc tggtgttgag
      961 cggggaagag ggttggtgtt ttgatcatgc gcttaagttg cgtgatgcct tacctggcga
     1021 ctggctgtgg atttcgccgc agccagatgc tgaaaaccac tgttcaccct cggcgctaca
     1081 aactttactt gggcgcgagt tccggcatgc ggtattcgac gcccgccacg gctttgatgc
     1141 cgctgccttt gcagcactta gcggaacgtt gaaagcggga agctggctgg ttttgttact
     1201 ccctgtatgg gaagagtggg aaaaccaacc tgatgccgac tcgttgcgct ggagtgattg
     1261 ccctgaccct attgcgacgc cgcattttgt ccagcatctc aaacgcgtac ttacggcgga
     1321 taacgacgct atcctctggc ggcaaaacca gccgttctcg ttggcgcatt ttactccccg
     1381 tactgactgg caccccgcga ccggcgcacc acaaccagaa caacagcaac tcttacagca
     1441 gctactgacc atgccgccgg gcgtggcagc ggtaacggct gcgcgtgggc gcggtaagtc
     1501 ggcgctggca gggcaactca tttctcgtat tgcgggcagt gcgattgtca ccgcgcccgc
     1561 aaaagcggca acggatgtac tggcacaatt tgcgggcgag aagtttcgct ttattgcgcc
     1621 ggatgccttg ttagccagcg atgagcaagc cgactggctg gtggtcgacg aagccgcagc
     1681 catacctgcg ccgttgttgc atcaactggt atcgcgtttt cctcgaacgt tgttaaccac
     1741 tacggtgcag ggctacgaag gcaccggacg tggttttttg ctgaaatttt gcgctcgctt
     1801 tccgcattta caccgttttg aactgcaaca gccgatccgc tgggcacagg gatgcccgct
     1861 ggaaaaaatg gttagtgagg cactggtttt tgacgatgaa aacttcaccc atacaccaca
     1921 aggcaatatc gtcatttccg catttgaaca aacgttatgg cgaagcgagc cagaaacgcc
     1981 gttaaaggtt tatcagttat tgtctggtgc gcactaccgg acttcgccgc tggatttacg
     2041 ccggatgatg gatgcaccag ggcaatattt tttacaggcg gctggcgaaa acgagattgc
     2101 cggagcgctg tggctggtgg atgagggggg attatctcaa caactcagtc aggcggtatg
     2161 ggcaggtttt cgtcgcccgc ggggtaatct ggtggcccag tcgctggcgg cgcacggtag
     2221 caatccactg gcagcgacat tgcgtggacg gcgggtcagc cggatagcag ttcatccggc
     2281 gcgtcagcgg gaaggcacag ggcagcaact tattgctggt gctttgcaat atacgcgtga
     2341 cctcgactat ctttcggtga gttttggtta caccggggag ttatggcgtt tctggcaccg
     2401 ctgcggtttt gtgctggtgc ggatgggtaa tcatcgtgaa gccagcagcg gttgctatac
     2461 ggcgatggcg ctgttaccga tgagtgatgc gggtaaacag ctggctgaac gtgagcatta
     2521 ccgtttacgt cgcgatgcgc aagctctcgc gcagtggaat ggcgaaacac ttcctgttga
     2581 tccactaaac gatatcgtgc tttctgacga cgactggctt gaactggccg gttttgcttt
     2641 tgctcatcgt ccgctattaa cgtcgttagg ttgcttattg cgtctgctac aaaccagtga
     2701 actggcatta ccggcgctgc gtgggcgttt acagaaaaat gccagtgatg cgcagttatg
     2761 taccacactt aaacttgcag gccgcaagat gttactggtc cgtcagcggg aagaggtcgc
     2821 gcatgcgctg ttcgcactta atgatgttcg cactgagcgt ctgcgcgatc gcataacgca
     2881 atggcaattt tttcactgac tccttcaatt ttctcccatg gtcattgtga gccagcggcg
     2941 taaaattaat gacatcagtt aaggagttcg ccatgaaaca tgaccatttt gttgttcaaa
     3001 gcccggataa acctgcgcaa cagttgttgc tgcttttcca cggtgtcggg gataacccgg
     3061 tggcaatggg ggaaataggt agctggtttg caccgctgtt tcctgatgcg ctggtggtga
     3121 gtgtcggtgg cgcggagccg agcggtaatc cggcggggcg tcagtggttt tcggtgcagg
     3181 gtattacgga agataatcgc caggcgcgtg tggatgcgat catgccgacg tttattgaaa
     3241 cggtgcgcta ctggcagaaa cagagcggag taggggctaa cgccacggcg ctcatcggtt
     3301 tctctcaagg cgcgattatg gcgctggaga gcattaaagc cgaaccgggc cttgcttcac
     3361 gcgtcattgc ttttaacgga cgctatgcga gcctgccgga aactgcgtct acagccacga
     3421 caattcacct gattcatggc ggtgaagatc cggttatcga tctggcgcat gcggttgcgg
     3481 cgcaggaagc gttaatcagt gccggtggtg acgtgacgct ggatatcgtg gaggatttag
     3541 gccatgcgat cgacaaaaat tgtcgaatta ttaacgtagg ttatcgcgtc tagtgaatcc
     3601 acgttctttc atgtcgttat acatcccccg ctttcattta tcgctatcct ttttctatcc
     3661 ttttaggcta aattaaccag catctggaac cagccagggg gattcaatga cgtcaggtaa
     3721 aaccaatctc tttcgcttca ccaagtcagc tattaaccag atcccgccat cgccaaaggg
     3781 caaacagctt gaatatcttg atacacaggt taaggggttg cgtctccgcg tgggatcaac
     3841 ggggaaaaag acgtttgtta ttgtcaggaa cggcaaggag ggatttatca gggtcaaggt
     3901 tggaaacttt cctgatatgt ccgttgaaac agcgagagcg gtcgccctga agaagctggg
     3961 tgatctctca ttaacgcgaa agaatccgaa caaagagaaa aaagcagccg aagaaagtaa
     4021 cgaagaaacc aacgaacaac gcaacgtaac gctaagggag gcgctcaatc agtatctgga
     4081 tctgaggggc gagcgcatca gcgagactac agccaaacaa tacacgtcct tgctgtccaa
     4141 tttttcgcgt gactggtttg atcttccttt ggcgaaaata tcaaggcgca aggtgacgac
     4201 aaggcacaag caaatcactg acggatcggt atggtttggt tcgccggaac agaaaaacaa
     4261 aaagaacgta gcgaaaggaa gcaaggcgca ggctgatttg tgggggcgag tactgagggc
     4321 cgtctaccgg tttgcgtatg attattacag ggatgacgac gaacaaaggt tacttcctga
     4381 acctccaacg caggcgctga cgtcacaaag acaatggaac ggttcgaaac ggaaaacaac
     4441 acgaataaga aaccacgaga ttgggcgatg gtacagagcg gttaatgctg ttctttgtga
     4501 tgctgaggct atcagcgact ttactgctat gtcggtatgt gatgcgctga tttcttcact
     4561 gtttacagga atgagaaagg gggaggttct cggcctcaca tggaataacg tagatcttga
     4621 aggtggatat atccggctac aagacacaaa aaacaaaaga gatacagaaa tacctataac
     4681 ccaaacgatg cgggaaataa ttacgcgtcg tgataagtgg cggatatatg ccagtgatta
     4741 cgtatatcct tcattctgtg gggagaagat agaaaaccca agaagaacga ttattcagat
     4801 tatggataaa acaacaatta ttgacggcga atataaagcg cctgttaaat tttgttttca
     4861 tgacctccgc cggacctttg ccagcattgc cgagctaagc ggcgtaggga tatatacaat
     4921 aaaaaaactc atgaatcaca aatctgggca aggtgcagat gtcacaattg gctatattaa
     4981 tatgacagct gacgaattat tacagccatc tcaaattatc gaaaagagaa tattaaagga
     5041 aattggcata gcaga
//
LOCUS       DAZFQD010000223         4794 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.224, whole genome shotgun sequence.
ACCESSION   DAZFQD010000223 DAZFQD010000000
VERSION     DAZFQD010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4794
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.224"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            102..815
                     /gene="ydiV"
                     /locus_tag="ACHKTW_004950"
     CDS             102..815
                     /gene="ydiV"
                     /locus_tag="ACHKTW_004950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HFO6465817.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQDLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLERDNYAG
                     ELLKYPFIELLINENYPHLNEGKDNRDLLSLSQMYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLDKSFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTTEILAQITPFDFHAL
                     QGCLWPAVPINQITTLVQR"
     gene            878..2314
                     /gene="selO"
                     /locus_tag="ACHKTW_004951"
     CDS             878..2314
                     /gene="selO"
                     /locus_tag="ACHKTW_004951"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase SelO"
                     /protein_id="HFO6465818.1"
                     /translation="MTLSFITRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNAAGVWGGETLLPGMSPLAQVYSGHQFGVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TVEPGAMLMRVAPSHLRFGHFEHFYYRREPEKVRQLADFAIRHYWSHLEDDEDKYRLW
                     FNDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLRDEFIDRAAFDDW
                     FARYRRRLQQDEVSDIERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMTELHRLHEAL
                     RNPFSDRADDYVSRPPDWGKRLEVSCSS"
     gene            complement(2318..2509)
                     /gene="hemP"
                     /locus_tag="ACHKTW_004952"
     CDS             complement(2318..2509)
                     /gene="hemP"
                     /locus_tag="ACHKTW_004952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HFO6465819.1"
                     /translation="MRYTDSRKLTPETDANHKTASPQPIRRITSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(2641..3687)
                     /gene="aroH"
                     /locus_tag="ACHKTW_004953"
     CDS             complement(2641..3687)
                     /gene="aroH"
                     /locus_tag="ACHKTW_004953"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HFO6465820.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVAIHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTAIAGIMAESFLREGTQKIVGGQPLTYGQSITDPCLGWEDTE
                     RLVEKLASAVDTRF"
     gene            complement(3844..4677)
                     /gene="ppsR"
                     /locus_tag="ACHKTW_004954"
     CDS             complement(3844..4677)
                     /gene="ppsR"
                     /locus_tag="ACHKTW_004954"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HFO6465821.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
BASE COUNT         1190 a         1128 c         1222 g         1254 t
ORIGIN      
        1 atggaacaaa tgaaagttac tccttatgta attgttatgt cttatggcct catccctttt
       61 agccggatac tgaaaaacat ccttcgagag ggacggttac catgaagatt tttttggaga
      121 atctttatca ttctgattgc tatttcctgc cgataagaga taaccagcaa gaccttgtcg
      181 gcgttgagtt aattacccat ttctccagtg aagatggtac ggttcgtatc ccgacgagtc
      241 gggttatagc gcaacttacc gaggagcaac attggcaatt attttcagag cagttggaat
      301 tactaaaatc atgccaacat ttttttattc aacataaact ctttgcctgg cttaatttaa
      361 cgccacaagt ggctacatta ttattagaga gggataatta cgcaggcgaa ctattaaaat
      421 atccgtttat tgaactgttg attaatgaga attacccgca tctcaatgaa ggaaaagata
      481 atcgcgatct tttatcctta tcgcaaatgt atccccttgt gttaggcaat ttgggagcag
      541 gtaatagcac aatgaaagcg gtttttgatg gtcttttcac ccgcgtcatg ctggataaaa
      601 gttttattca gcaacaaatc acgcatcgct ccttcgaacc gtttattcgt gcgatccagg
      661 cgcaaatttc cccttgttgc aattgcatca tcgcgggggg cattgatacg acagaaatat
      721 tggcacagat tacccccttc gatttccatg ctctccaggg atgcctgtgg cctgccgttc
      781 ctataaatca gataacgacg ttggttcagc gataaccctc ctgtttgctg gtgtttaaga
      841 cgagagtaac cgtctacact atcaaacagg aggatctatg accctgtctt ttattacccg
      901 ctggcgcgat gaattgccag aaacctatac agcactttcc cctacgcctt taaataatgc
      961 ccggctgatt tggcataata ccgaactcgc taacacgctg agtattccat cgtcgctgtt
     1021 taaaaatgct gcaggtgtct ggggcggcga aaccctactg cctggtatgt caccactggc
     1081 gcaggtttac agtggtcatc agttcggcgt ctgggcgggc caactgggtg atgggcgcgg
     1141 cattttactt ggcgaacaac tgcttgctga tggcactaca atggactggc atctgaaagg
     1201 tgctggcctg acgccttatt cgcgaatggg tgatggacgg gcggttttac gttcgacgat
     1261 acgagaaagt ctcgccagtg aggcgatgca ttatctgggc attccgacga cccgcgcgtt
     1321 aagtatcgtc accagcgatt cgccagtgta tcgggaaacg gtagagccag gtgcgatgct
     1381 gatgcgtgtg gcaccaagtc atctgcgctt tggtcatttc gaacattttt actatcgccg
     1441 cgagccggaa aaggttcgtc agttagctga ctttgccatt cgtcattact ggtcacatct
     1501 tgaagatgat gaggataaat accgtctctg gtttaacgat gttgtcgcac gtaccgcatc
     1561 gttaattgcc caatggcaga cggtcggctt tgctcatggg gtgatgaata ccgacaacat
     1621 gtcgctgctg gggctgacgc ttgattacgg gccgtttggt tttcttgatg attacgaacc
     1681 cggttttatt tgtaatcact cggatcatca agggcgttac agctttgata atcaacctgc
     1741 cgtcgcgttg tggaatttac agcgtctggc gcagacattg tcaccatttg ttgccgtaga
     1801 tgccctgaat gaggccctgg acagctatca gcaggttttg ttgacgcatt atggacaacg
     1861 gatgcggcag aaactgggct tcatgacgga gcaaaaagag gataacgcgc tactgaatga
     1921 attattcagt ctgatggcgc gagagcgcag cgattatacc cgcacattcc gcatgctgag
     1981 tctgaccgag cagcacagcg cggcgtcacc gctacgtgat gagtttattg atcgtgcggc
     2041 atttgatgac tggtttgccc gttatcggag gcgtttgcaa caagacgagg ttagcgatat
     2101 tgagcgtcag caactgatgc aaagcgttaa cccagctctg gtgttgcgca actggttggc
     2161 gcaacgggcg attgaagcgg cagaaaaggg tgatatgacg gaattgcacc gcctgcatga
     2221 ggcgttgcga aatcctttca gcgacagagc tgatgactat gtcagtcgtc cacctgactg
     2281 gggtaaacgg ctggaagtca gctgctcgag ttaaaggcta cttggtcaac agcagcttgc
     2341 cagcctgagt tttacggagc agatattctt gcccgtcatg atcgataatc agtttgccat
     2401 ccggacctaa cagtgtctgg ctggtaattc gccggatggg ctgcggggaa gcggtcttgt
     2461 gattggcatc cgtttcaggc gtgagttttc tgctatccgt ataacgcata aaaattaata
     2521 ataattgata taacaatgat tcttattatc aatattatgg caggccagag caagcagtaa
     2581 tgtgttgaaa agacaacaag ttgcgcagaa atgcccatcc catcaggaat gggcacgcat
     2641 tcagaagcgg gtatctaccg cagaggcgag tttttcgacc aggcgttcgg tatcttccca
     2701 gcccagacac gggtcggtaa tggattgacc gtaagtgagc ggctgaccgc cgacgatttt
     2761 ttgcgttcct tcgcgcagga aactttccgc cataattcca gcaatcgccg tagagccatt
     2821 gcggatttgc tgacaaatat cctcacaaac ttctaactgg cgacggtgct gcttctggca
     2881 gttaccgtgg ctgaaatcca ccaccagatg ttcaggtaaa tcaaactcgt gcagcgtatc
     2941 gcaggctgcg gcgatatcat cggcatgata attcggtttt ttgccgccac gcataataat
     3001 gtggccatac gggttgccgc tggtctgata gatggtcatc tgaccatttt tgtctggcga
     3061 gagaaacata tggctggcgc gggctgcgcg gatagcatcc acggcaatcc gcgtgttgcc
     3121 atcggtacca tttttaaaac ctaccggaca ggagagtgcc gaagccattt cacggtggat
     3181 ctgactttcg gtagtacgtg cgccaatcgc gccccaactg attaaatcag caataaactg
     3241 accggtcacc atatcgagga actcagtcgc ggtagggaca cccagctcat ttacctgtaa
     3301 aagtaatttg cgcgccagct ccagaccgtg atttacccga tagctgccgt ttaaatccgg
     3361 atcggagatt agccctttcc agccgacaac agttcgtggt ttttcaaaat aggtgcgcat
     3421 tacgatttcc agccgtgact ggtactggtt gcgcagcgac tgcagacggg tggcgtactc
     3481 cattgcagcg gtgagatcgt ggatcgagca ggggccaata ataaccaaca gtcgcttatc
     3541 ttcaccattc agtatttttt caattctgcg gcgggagtcg gtgacatgga tggcgacgcc
     3601 aggcgttacg ggataccgta gcgcgagttc ggcgggcgtt accaggctct caatacgcgc
     3661 agtacggagt tcgtcagttc tgttcattac aagtctcaga aatttgttgc ttctacggca
     3721 ggactaccgg gaagtgatgc gcatcacgat aaaccaatcg cagctgattt caagtgcgag
     3781 gtgtgtcaca cctcgccggg tggttagcat gataacaaaa aaataagcta atgcactagt
     3841 tctctagtac attcggcggc taagccccat gatatcgagg atcttggtgg caatctcttc
     3901 taccgaataa ttggtactgt taatccacgg gatctgattt ttacggtaca acgcttctac
     3961 ttccgcgact tccatcctgc actgacgaag cgaggcatag cggctgttct cccgacgttc
     4021 ctcgcgaatc gccgccagac gttccgggtc gatagtcagg ccgaacaatt tatgctgaag
     4081 cggtttgagc gacgcgggta gcaccagatt atccatatcg tcggcaataa aggggtagtt
     4141 tgccgcgcgg ataccaaatt gcattgccag atacagactg gtgggggttt taccacagcg
     4201 agaaacgccg agcaggatca cctgagcctg gtccagattg cgcaacgaaa taccgtcatc
     4261 gtgggcaagg gtgtaatcaa tcgccgcaat gcgcgcatca tatttattga gattattagg
     4321 gttaaggcca tgggtacgat gagcaatcgg cgttggatcc agtttcatct cttgttgtag
     4381 cggggcaacc agcgcctgaa cgatatcctg gcaaaagcct tcactttgca agatgatggc
     4441 gcgaatctcc ggcaacacga tggagtagaa gaccagcggg cgcacacctg tctggtgata
     4501 aatggcgtca atctgatcct tcactgcccg tgcacggctc tcattttcga caaacggcag
     4561 cgtaatgctg ctgatagtga cgggaaattg tgacattact gcgtgtccta atacctccgc
     4621 agttattgcc gtaccatcag aaatataaaa aacgtggcga tcaacagcat tatccatttt
     4681 gttcttcccg tgatgcagac ataattctat gaaagcataa attaaaaacg cacagaagcg
     4741 taggacgtaa tgtctggttt ataaaatgaa ccttcaattt tattttttat gaaa
//
LOCUS       DAZFQD010000224         4750 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.225, whole genome shotgun sequence.
ACCESSION   DAZFQD010000224 DAZFQD010000000
VERSION     DAZFQD010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4750
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.225"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..836
                     /gene="dgaE"
                     /locus_tag="ACHKTW_004955"
     CDS             <1..836
                     /gene="dgaE"
                     /locus_tag="ACHKTW_004955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="D-glucosaminate-6-phosphate ammonia lyase"
                     /protein_id="HFO6465822.1"
                     /translation="VALMPDSSGMANEVVMLRGHNVDYGASILSAIRLGGGRVVEVGS
                     SNLAALWQLESAITDATAALLYVKSHHCVQKGMLNIEDFAHVAQMYNLPLIVDAAAEE
                     DLRAWVVSGADMVVYSGAKAFNAPTSGFITGKKIWIAACKAQHHGIARAMKIGKENMV
                     GLVYALENYHQGQAVITAEQLQPVVEAISAIHGLTADIEQDEAGRAIWRIRIRVNAQE
                     LGVDARVVEAQLRGGDIAIYARRYNLHQGVFSLDPRTVAEGEMALIVARLKEIADHAK
                     D"
     gene            823..1566
                     /locus_tag="ACHKTW_004956"
     CDS             823..1566
                     /locus_tag="ACHKTW_004956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFO6465823.1"
                     /translation="MQKINFYRNRVAINVLAKDIANAREIFDAAEGHAVIGVLSAQFS
                     SVDEGIQEVKRWMAEIPSISVGLGAGDPAQYYKAAMIAAQIHPAHVNQTFTGCGFAAG
                     ALAATGGEQTHVNALVSPTGTPGEVLISTGVSSSQGTPARVSCDTAVRMMLDMGAHAA
                     KFFPMGGERSLPELYALATTAARNGMTLIEPTGGIDLDNFSVILKTCLEAGVPRIMPH
                     VYSSIIDPDTGYTRPDDVAVLLNKVKSLL"
     gene            complement(1622..2380)
                     /gene="loiP"
                     /locus_tag="ACHKTW_004957"
                     /gene_synonym="yggG"
     CDS             complement(1622..2380)
                     /gene="loiP"
                     /locus_tag="ACHKTW_004957"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HFO6465824.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLRQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRMSADGVK"
     gene            2658..4649
                     /gene="tkt"
                     /locus_tag="ACHKTW_004958"
     CDS             2658..4649
                     /gene="tkt"
                     /locus_tag="ACHKTW_004958"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFO6465825.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIVGMTTFGESAPAELLFEEFGFTVDNVVAKAKELL"
BASE COUNT         1064 a         1252 c         1350 g         1084 t
ORIGIN      
        1 gggtggcgct catgccggat agctccggca tggccaatga agtggtgatg ctgcgcggcc
       61 ataacgtcga ttacggtgca tcgatcctca gcgcgatccg cttaggtggc gggcgcgttg
      121 tggaggtggg ttcaagtaac ctggcggctc tctggcagct ggagagtgcc attaccgacg
      181 cgaccgccgc gctgctgtat gtgaagtcgc atcactgcgt gcagaaggga atgttgaaca
      241 tcgaggactt cgcacatgtc gcgcagatgt acaacctgcc gctgattgtc gatgccgccg
      301 ccgaagagga tttacgtgcc tgggtggtga gcggagcgga tatggtggtc tacagcggcg
      361 caaaagcctt taacgcgccg acctccgggt ttatcactgg taaaaaaatc tggattgcag
      421 cctgcaaagc gcagcatcac ggcattgcca gggcgatgaa aatcggcaag gagaacatgg
      481 ttgggctggt ttacgcgctg gagaattatc accaggggca ggcagtcatc accgcggaac
      541 agctccagcc agtggttgaa gcgatttctg cgattcacgg gttaacggct gatattgagc
      601 aggatgaagc cgggcgtgca atctggcgta ttcgtattcg agtaaatgcg caggaattgg
      661 gagttgacgc tcgcgtcgtc gaagcgcaac tgcgtggtgg cgatatcgcg atttatgcac
      721 ggcggtataa cctccaccag ggcgttttta gccttgatcc gcgtacggtg gcggaagggg
      781 aaatggcact tatcgtggcg cgactgaagg agattgcaga tcatgcaaaa gattaatttc
      841 tatcgtaatc gcgtggcaat taatgtgctg gcgaaagata tcgccaatgc ccgtgaaatc
      901 tttgacgccg ccgaaggcca cgcagtgatt ggcgtgcttt ccgcgcagtt ctcctcggtg
      961 gatgagggga tccaggaagt taaacgttgg atggcggaaa taccgtccat ttcagtaggt
     1021 ctgggggcgg gtgacccggc gcagtattac aaagctgcga tgattgcggc gcagattcat
     1081 ccagcacacg ttaaccagac ctttaccggt tgtggtttcg cggcaggcgc gcttgcggct
     1141 acgggcggtg agcagaccca tgtcaacgcg ctggttagcc cgacggggac gccgggcgaa
     1201 gtgctgattt ccaccggcgt tagcagcagc cagggtacgc ctgcccgcgt ctcctgcgat
     1261 acggcggtgc gaatgatgct agatatgggc gcacacgcgg cgaagttctt tccgatgggc
     1321 ggcgagaggt cgcttccgga gctatacgcg ctggcgacca cggcggcgcg caatggtatg
     1381 acgctgattg agccgactgg cgggattgat ttggataact tcagtgtgat tctgaaaact
     1441 tgtctggagg cgggggtgcc gcgcattatg ccacatgttt acagctcgat tatagaccct
     1501 gatacgggat atacccgacc ggatgatgta gccgttttgc taaataaagt gaaatcacta
     1561 ctctgatcgg ggattcaggc gttaccgacc tacaattatg ttgtagatcg gtatgtctga
     1621 cttacttaac cccatccgcg ctcatacgat cgcgaatatg ctgggcgcgt tcggcggatg
     1681 caggatggtc gtcaaacatt gagctttggc gaccttcttc cagttttgcc agtttttcaa
     1741 agctggtggc aagacctgcc gggctgatgc cgcgttggcg cagaagatcg taagaataat
     1801 catcggcttc tgcttcctgg cgctgggaga attgcgaatt gactaatttc tcgcccagat
     1861 caccaagttg tgattgagat aaactgccga caatcccgcc cgcagaggcc gcagctactc
     1921 gcacggcatt tgtaccaagc gccacctgca tccctttctt cacatggcct aacgccacgt
     1981 gccccatctc gtgaccgatc accgcttcga cttcgttatc cgtcatcata tccatcagcc
     2041 cgctatagac gcggatacag ccgttagcca ttgcaaaggc gttcacatcc ttcgccatat
     2101 acactttgta attcaccggc tgaccgttga tattgttgcc tagcgcattg gcaatagttg
     2161 tcagacgttt agcgtattcg ctattggctg gcgcaatcgt cgccttgctg tccatctcct
     2221 gacatgcctg atcgctcagg gttttcacct gcgcatcact caaactgtaa gcctgaaaag
     2281 cttccgctcc tgatgagagc agtccgttgg agtccatatt ctggcaacca gtcagtaccg
     2341 ttgccacgct cattgctacc aataaggcgc gaattttcat gttttctctt ccgcacaagg
     2401 tcaggattat tctaaaaatg cgccgtttgc aggtgaatcg acgctcagtc tcagtataag
     2461 gaaaagcgca gcggacgggc gagtagattg cgcaacatgc gagcatgatc cagagatttc
     2521 tgaagcggca aaaggatgtt ccatgtacat gacgcgcggc ttgcggtaaa ttgttggcaa
     2581 attttccggc gtagcccaaa acgcgctgtc gtcaagtcgt taagggcgtg cccttcatca
     2641 tccgatctgg agtcaaaatg tcctcacgta aagagcttgc caatgctatt cgtgcgctga
     2701 gcatggacgc agtacagaaa gccaaatccg gtcacccggg tgcccctatg ggtatggctg
     2761 acattgccga agtcctgtgg cgtgatttcc tgaaacacaa cccgcagaat ccgtcctggg
     2821 ctgaccgtga ccgcttcgtg ctgtccaacg gccacggctc catgctgatc tacagcctgc
     2881 tgcacctcac cggttacgat ctgccgatgg aagaactgaa aaacttccgt cagctgcact
     2941 ctaaaactcc gggtcacccg gaagtgggtt acaccgctgg tgtggaaacc accaccggtc
     3001 cgctgggtca gggtattgcc aacgcagtcg gtatggcgat tgcagaaaaa acgctggcgg
     3061 cgcagtttaa ccgtccgggc cacgacattg tcgaccacta cacctatgcc ttcatgggcg
     3121 acggctgcat gatggaaggc atctcccacg aagtttgctc tctggcgggt acgctgaagc
     3181 tgggtaaact gattgcgttc tacgatgaca acggtatctc tattgatggt cacgttgaag
     3241 gctggttcac cgacgacacc gcaatgcgtt tcgaagctta cggctggcac gttattcgcg
     3301 acatcgacgg tcatgacgcg gcatccatca aacgcgcagt agaagaagcg cgcgcagtga
     3361 ctgacaaacc gtccctgctg atgtgcaaaa ccatcatcgg tttcggttcc ccgaacaaag
     3421 ccggtactca cgactcccac ggtgcgccgc tgggcgacgc tgaaattgcc ctgacccgcg
     3481 aacagctggg ctggaaatat gcaccgttcg aaatcccgtc tgaaatctat gctcagtggg
     3541 atgcgaaaga agcaggccag gcgaaagaat ccgcatggaa cgagaaattc gctgcttacg
     3601 cgaaagctta tccgcaggaa gccgctgaat ttacccgccg tatgaaaggc gaaatgccgt
     3661 ctgacttcga cgcgaaagca aaagagttca ttgctaaact gcaggctaac ccggcgaaaa
     3721 tcgccagccg taaagcgtct cagaatgcta tcgaagcttt cggtccgctg ttgccggaat
     3781 tcctcggcgg ttctgctgac ctggcaccgt ctaacctgac cctgtggtct ggttctaaag
     3841 caatcaacga agatgctgcg ggtaactaca tccactacgg tgttcgcgag ttcggtatga
     3901 ccgcgattgc taacggtatc tccctgcacg gtggtttcct gccgtacacc tccaccttcc
     3961 tgatgttcgt ggaatacgca cgtaacgccg tacgtatggc tgcgctgatg aaacagcgtc
     4021 aggtgatggt ttacacccac gactccatcg gtctgggcga agacggcccg actcaccagc
     4081 cggttgagca ggtcgcttct ctgcgcgtaa ccccgaacat gtctacatgg cgtccgtgtg
     4141 accaggttga atccgcggtc gcgtggaaat acggtgttga gcgtcaggac ggcccgaccg
     4201 cactgatcct ctcccgtcag aacctggcgc agcaggaacg aactgaagag caactggcaa
     4261 acatcgcgcg cggtggttat gtgctgaaag actgcgccgg tcagccggaa ctgattttca
     4321 tcgctaccgg ttcagaagtt gaactggctg ttgctgccta cgaaaaactg actgccgaag
     4381 gcgtgaaagc gcgcgtggtg tccatgccgt ctaccgacgc atttgacaag caggatgctg
     4441 cttaccgtga atccgtactg ccgaaagcgg ttactgcacg cgttgctgta gaagcgggta
     4501 ttgctgacta ctggtacaag tatgttggcc tgaacggtgc tatcgtcggt atgaccacct
     4561 tcggtgaatc tgctccggca gagctgctgt ttgaagagtt cggcttcact gttgataacg
     4621 ttgttgcgaa agcaaaagaa ctgctgtaat tagcatttcg ggtaaaaagg tcgcttcggc
     4681 gacctttttt attaccttga tatgtccgtt tgcggacaag caatagataa agcgtgttgt
     4741 agatcacaaa
//
LOCUS       DAZFQD010000225         4712 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.226, whole genome shotgun sequence.
ACCESSION   DAZFQD010000225 DAZFQD010000000
VERSION     DAZFQD010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4712
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.226"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(19..1029)
                     /gene="pyrD"
                     /locus_tag="ACHKTW_004959"
     CDS             complement(19..1029)
                     /gene="pyrD"
                     /locus_tag="ACHKTW_004959"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HFO6465826.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEEELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            complement(1140..1877)
                     /locus_tag="ACHKTW_004960"
     CDS             complement(1140..1877)
                     /locus_tag="ACHKTW_004960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HFO6465827.1"
                     /translation="MPARHLYFIMTNTWNRLALLIFAVLSLLVAGELQAGVVVGGTRF
                     IFPADRESISILLTNTSQESWLINSKINRPTRWAGGEASTVPAPLLAAPPLILLKPGT
                     TGTLRLLRTESDILPVDRETLFELSIASVPSGKVENQSVKVAIRSVFKLFWRPEGLPG
                     DPLEAYQQLRWTWNSQGVQLTNPTPYYINLIQVSVNGKALSNAGVVPPKSQRQTSWCQ
                     AIAPCHVAWRAINDYGGLSAKKEQNLP"
     gene            complement(1843..2358)
                     /locus_tag="ACHKTW_004961"
     CDS             complement(1843..2358)
                     /locus_tag="ACHKTW_004961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO6465828.1"
                     /translation="MLKRIIWILFLLGLTGGCELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFFHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTPDGVQSEMLSI
                     ESGTDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            complement(2366..2908)
                     /locus_tag="ACHKTW_004962"
     CDS             complement(2366..2908)
                     /locus_tag="ACHKTW_004962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO6465829.1"
                     /translation="MKKKTIFQCVILFFSILNIHVGMAGPEQVSMHIYGNVVDQGCDV
                     ATKSALQNIHIGDFNISDFQAANTVSTAADLNIDITGCAAGITGADVLFSGEADTLAP
                     TLLKLTDTGGSGGMATGIAVQILDAQSQQEIPLNQVQPLTPLKAGDNTLKYQLRYKST
                     KAGATGGNATAVLYFDLVYQ"
     gene            complement(2920..3990)
                     /locus_tag="ACHKTW_004963"
     CDS             complement(2920..3990)
                     /locus_tag="ACHKTW_004963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO6465830.1"
                     /translation="MQIIFGEKCVLLLRLFFAAVLMLWCAQTAAYSGQCHTTQGNPYI
                     GVNFGVKTLEEEENTAGVVKDKFYQWNESNDYYVSCDCDKDNVRSGRWAFAADSPLVY
                     LGDNWYKINDYLAAKVLLQVKGSSPTAVPFENVGTGADTRWHICDPGGQRLGGQGASG
                     NSGSFSLKILQPFVGSVVIPPMALARLFECYNIPAGDSCTTTGTPVLVYYLSGTINSL
                     GSCSVNAGETIEVDLGDVFAANFRVVGHKPLGARTAELAIPVRCNTGNAGLVNVNLSL
                     TATTDPSYPQAIKTSRPGVGVVVTDSQNNIISPAGGTLPLSIPDDADSIARMNVYPVS
                     TTGVPPETGRFEATATVRINFD"
     gene            complement(3981..>4712)
                     /locus_tag="ACHKTW_004964"
     CDS             complement(3981..>4712)
                     /locus_tag="ACHKTW_004964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFO6465831.1"
                     /translation="GLTETLLDDGNLSYSVQQGYNSEGKTANGSASMDYKGAFADARV
                     GYNYSDNGSQQQLNYALSGSLVAHSLGITLGQSLGETNVLIAAPGAENTRVANSTGLK
                     TDWRGYTVVPYATSYRENRIALDAASLKRNVDLENAVVNVVPTKGALVLAEFNAHAGA
                     RVLMKTSKQGIPLRFGAIATLDGIQTNSGIIDDDGSLYMSGLPAQGAITVRWGEAPDQ
                     ICHISYQLTEQQINSAITRMDAICR"
BASE COUNT         1189 a         1232 c         1071 g         1220 t
ORIGIN      
        1 aggaattcga agaaataatt agatatgggt aacgatttct ttaatcagcg gcggaccttt
       61 aaaaataaaa ccagaataaa tttgcaccag tgaggcaccc gcagcaatct tttcacgcgc
      121 agcgataacc gagtcgatgc cgccaacacc gatgatcggt aagcgaccgt ttaattccag
      181 tgacaagcgg cgaataattt cggtgctttt taactgaagc ggacgaccac ttaagccacc
      241 ggtttgatcg caatttttca ttccctgaac aagagaacga tcgagtgtgg tattggttgc
      301 aataacgcca tcaatattat ggcgaactaa actatcggca acctggatca attcttcttc
      361 agaaagatcc ggcgcgatct tcactgcgat cggcacatat ttatggtgca tcgcttgcaa
      421 atcattttgc ttatttttaa tcgcggttaa gagatcatcc agcgcttcac catattgcag
      481 tgtgcgtaat cctggggtat tcggcgatga aatattgatg gcgatatatc ccgcataggc
      541 atagattttt tccatacaaa tcagatagtc atctttgccc tgctccactg gcgtatcttt
      601 atttttgccg atgttaatac ccaggacgcc gtcataatgg gcctttttta cgttctctac
      661 gaggttatca acgccaagat tattaaagcc catacggttg atcaaacctt cggcatctac
      721 cagacgaaag agacgcggct tgtcattacc tggctgtgga cgtggcgtga cggtaccgat
      781 ctcgatcgat ccaaatccca tcgcgcctaa cgcgtcaatg cactccccgt ctttatcaag
      841 accggctgcc agaccaagcg gatttttaaa cgtcaggccc atgcagttaa caggtttcgc
      901 aggcactttc tgccgcacca gtgcttcaaa cggcgttcct gtaatacggc gtaattgctg
      961 aaaagtaaac tcatgagcgc gctctggatc gagctggaaa agggctttac gaacgaaggg
     1021 gtagtacatg aactctcctg gattcccggt gtgcaaaccg ggggcgtatt atgtgcgagt
     1081 tcggaccaaa agggaattga cctgcggcaa aaaaaacgca aacgttttcc gaacctgttt
     1141 cagggcagat tttgctcctt ctttgcactc aacccgccgt aatcatttat cgcccgccag
     1201 gcgacatgac acggtgcgat tgcctgacac cagcttgtct gacgctggct tttaggcggc
     1261 acaactcccg cattacttaa cgctttacca tttacgctca cctgaatcag gttaatgtag
     1321 taaggcgttg ggttagtgag ttgtacaccc tgcgaattcc atgtccagcg taattgttga
     1381 taagcttcca gcgggtcgcc cggcaaacct tcgggtcgcc agaacagttt aaataccgag
     1441 cgtatcgcca cttttacgct ctgattttca actttgccgg atggcacgct ggcaatgctt
     1501 aactcaaata gcgtttcgcg atccacaggc aagatgtcgc tttccgttct cagcaagcgc
     1561 aacgtgcctg tagtaccggg cttcaggaga ataagtggcg gagcggccag taatggtgct
     1621 ggcactgtcg acgcttcccc cccggcccaa cgcgttgggc ggttgatttt gctgttaata
     1681 agccaggatt cctgactggt attagtcagt aaaatagata tcgattctct gtctgccgga
     1741 aagataaatc gcgttccgcc gaccaccact cctgcctgca attcacccgc caccagcagc
     1801 gataaaacgg cgaaaatcaa gagcgccaat ctgttccacg tattagtcat aatgaagtac
     1861 aaatgtcgcg ctggcattca ctttacccga ctgcacatcg ctattgacag gtcgcaattg
     1921 cgcataaaaa gttaatggca ccttaccgct atgtaggctg tagtctttac tcgcctggtt
     1981 aagcggaatc aatatcttcg catcatccag aatcgcaatt gccagcccgc tggctgcatc
     2041 cgtaccactt tcaatggaca gcatttcgct ctgcactccg tcgggggttc ctgaaaaggt
     2101 gacgtcaacc gtgctgacat cagttccgca atcctgcaaa ttaatgatga aagatttctc
     2161 cgggccataa ttcccatgaa aaaaacgtgt cagcgagaca tcgcctaact gcacattgat
     2221 ttgcttgcta tcctgtgcca gcacgcatgt attacggcga aacgatccgc taatgttgac
     2281 tgtgccatca tgggcaaata gctcacagcc ccccgttaat cccagtaaga acagaatcca
     2341 gattatccgt ttcaacatca ctccttcact ggtaaaccag atcaaaatag agaaccgccg
     2401 tcgcattacc gcccgttgct cccgcctttg tggacttata acgaagttga tatttgagtg
     2461 tgttatcccc ggcttttaag ggcgtaagag gctggacctg attgagcggg atttcttgct
     2521 gactttgcgc atcaagaatt tgcacggcaa tccccgttgc cataccaccg cttccgcctg
     2581 tgtcagttag tttgagcagt gtcggcgcaa gggtgtcagc ctcgccgcta aaaaggacgt
     2641 ccgcgccagt aataccagcg gcacaaccgg tgatatcgat attcaaatca gcagcagtgc
     2701 ttacggtatt cgcggcctga aaatcactga tattaaaatc accaatatga atattttgta
     2761 atgcactttt ggtggcgaca tcacagccct gatcgaccac attcccataa atatgcatac
     2821 taacttgttc aggcccagcc atcccgacat ggatgttaag aatgctaaag aacaagataa
     2881 cgcactgaaa tatcgttttt ttcttcatac gttcacgcgt taatcaaaat ttattctcac
     2941 cgttgccgtg gcttcaaatc gcccggtttc tggtggtaca cctgtcgtgc tgactggata
     3001 gacattcatt cgcgcgatac tgtctgcatc atcaggaata gagagcggta atgttccacc
     3061 agcaggggaa ataatgttgt tctggctatc ggtcaccacc acgcccacgc caggacgtga
     3121 cgtcttaatc gcctggggat agctggggtc tgtggttgcc gtcagactca ggttgacgtt
     3181 aactaacccc gcgtttcccg tgttacacct gactggaatt gcaagttctg ccgttctggc
     3241 cccaagaggc ttatgcccta caacacgaaa gttagccgca aatacgtcgc ccagatcgac
     3301 ctcgattgtt tctccggcat tgacggaaca tgagccaagt gaattgatag taccagacag
     3361 gtaatacact aaaaccggtg tacctgtagt cgtgcaggaa tcacctgcgg gtatgttgta
     3421 gcattcaaat aatcgcgcca gcgccatagg aggaatgacg accgaaccaa cgaacggctg
     3481 caatattttc agggaaaagc taccgctatt accgctagcc ccctggccac ctaaacgttg
     3541 accgccggga tcgcaaatat gccatcgtgt atctgcccct gtgcccacgt tttcgaaagg
     3601 aaccgcagta ggagaactgc ctttaacctg caataaaact ttggcggcaa gatagtcatt
     3661 aattttgtac cagttgtcgc ctaaatagac taacggtgaa tccgcggcga atgcccatcg
     3721 gccacttctg acattgtctt tatcgcaatc acaggaaaca taataatcat tcgattcgtt
     3781 ccactgataa aatttgtctt taactactcc agccgtattt tcttcttcct ccagggtttt
     3841 aacgccaaaa ttgacgccaa tatacggatt cccctgagtg gtatgacact gcccgctata
     3901 agcagcagtt tgagcgcacc atagcattaa gacggcggca aaaaatagtc gtagtaataa
     3961 cacgcatttt tctccaaaga ttatctgcat atggcatcca ttcgcgtaat cgcagagtta
     4021 atttgttgtt cggtaagctg gtaactgata tgacaaattt gatcgggagc ttcgccccag
     4081 cgtacagtta tcgctccctg agcgggcaaa ccggacatat agagcgagcc atcatcatca
     4141 ataatgccgc tattagtctg tatgccgtcc agcgtcgcta tcgcgccaaa acgcagcggt
     4201 ataccctgct ttgatgtttt cattaatacc cttgctcccg catgggcatt gaactccgcc
     4261 agaaccaacg cccctttggt gggaaccacg ttgactactg cattttcaag atccacgtta
     4321 cgttttaacg atgccgcatc aagtgcgatt cgattttccc gataagaagt ggcatagggg
     4381 acaacggtat atccgcgcca gtcagttttc agcccggtgc tgttcgccac acgagtattc
     4441 tctgcgcctg gtgctgcaat caggacgtta gtttccccca gcgattgccc cagggtaatg
     4501 cccagtgaat gggcaactaa actgcctgaa agagcgtagt tcagttgttg ttgactgccg
     4561 ttatcgctgt agttgtagcc cactcgggca tctgcaaacg cccctttgta gtccatgctg
     4621 gcgctaccat tagccgtttt cccctcgctg ttatatccct gttgcacgct gtagctcagg
     4681 ttaccgtcat cgagtagtgt ctccgttaaa cc
//
LOCUS       DAZFQD010000226         4710 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.227, whole genome shotgun sequence.
ACCESSION   DAZFQD010000226 DAZFQD010000000
VERSION     DAZFQD010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4710
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.227"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1887
                     /gene="mngA"
                     /locus_tag="ACHKTW_004965"
     CDS             <1..1887
                     /gene="mngA"
                     /locus_tag="ACHKTW_004965"
                     /EC_number="2.7.1.195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HFO6465832.1"
                     /translation="ALCLNARFTSREEAIHALTQRLAALGKISSTEQFLEEVYRRESL
                     GPTALGEGLAVPHGKTAAVKEAAFAVATLSEPLQWEGVDGPEAVDLVVLLAIPPNEAG
                     TTHMQLLTALTTRLADDEIRARIQSATTPDELLSALDDKGGTQPSASFSNAPTIVCVT
                     ACPAGIAHTYMAAEYLEKAGRKLGVNVYVEKQGANGIEGRLTADQLNSATACIFAAEV
                     AIKESERFNGIPALSVPVAEPIRHAEALIQQALTLKRSDETRTVQQDTQPVKSVKTEL
                     KQALLSGISFAVPLIVAGGTVLAVAVLLSQIFGLQDLFNEENSWLWMYRKLGGGLLGI
                     LMVPVLAAYTAYSLADKPALAPGFAAGLAANMIGSGFLGAVVGGLIAGYLMRWVKNHL
                     RLSSKFNGFLTFYLYPVLGTLGAGSLMLFVVGEPVAWINNSLTAWLNGLSGSNALLLG
                     AILGFMCSFDLGGPVNKAAYAFCLGAMANGVYGPYAIFASVKMVSAFTVTASTMLAPR
                     LFKEFEIETGKSTWLLGLAGITEGAIPMAIEDPLRVIGSFVLGSMVMGAIVGAMNIGL
                     STPGAGIFSLFLLHDNGAGGVMAAIGWFGAALVGAAISTAILLIWRRHAVKHGNYLTD
                     GVMP"
     gene            1905..4538
                     /gene="mngB"
                     /locus_tag="ACHKTW_004966"
     CDS             1905..4538
                     /gene="mngB"
                     /locus_tag="ACHKTW_004966"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HFO6465833.1"
                     /translation="MKAVSRVHITPHMHWDREWYFTTEESRILLVNNMEEILCRLEQD
                     NEYKYYVLDGQTAILEDYFAVKPENKDRVKKQVEAGKLIIGPWYTQTDTTIVSAESIV
                     RNLMYGMRDCLAFGEPMKIGYLPDSFGMSGQLPHIYNGFGITRTMFWRGCSERHGTDK
                     TEFLWQSSDGSEVTAQVLPLGYAIGKYLPADENGLRKRLDSYFDVLEKASVTKEILLP
                     NGHDQMPLQQNIFEVMDKLREIYPQRKFVMSRFEEVFEKIEAQRESLATLKGEFIDGK
                     YMRVHRTIGSTRMDIKIAHARIENKIVNLLEPLATLAWTLGFEYHHGLLEKMWKEILK
                     NHAHDSIGCCCSDKVHREIVARFELAEDMADNLLRFYMRKIADNMPQSDADKLVLFNL
                     MPWPREEVINTTVRLRASQFNLRDDRGQPVPYFIRHAREIDPGLIDRQIVHYGNYDPF
                     MEFDIQINQIVPSMGYRTLYIEANQPGNVIAAKSDAEGILENAFWQIALNEDGTLQLV
                     DKDSGVRYDRVLQIEESSDDGDEYDYSPAKEEWVMTSATAKPQCEITHEAWQSRAVIR
                     YDMAVPLNLLERSVRQSTGRVGVEMVVTLSHNSRRIDVDINLDNQADDHRLRVLIPTS
                     FNTDSVLADTQFGSLTRPVNDSAMNNWQQEGWKEAPVPVWNMLNYAALQEGRNSMAVF
                     SEGLREFEVIGEEKKTFAITLLRGVGLLGKEDLFLRPGRPSGIKMPVPDSQLRGLLSC
                     RLSLLSYTGTPTAAGVAQQARAWLTPVQCYNKIPWDAMKLNKAGFNVPESYSLLKMPP
                     VGCLISALKKAEDRQEVILRLFNPAESATCDATVAFSREVISCSETMMDEHITTEENQ
                     GSNLSGPFLPGQSRTFSYRLA"
BASE COUNT         1145 a         1111 c         1314 g         1140 t
ORIGIN      
        1 gcgttgtgtc tgaatgcgcg ctttaccagc cgtgaagagg ccatccacgc gttgactcaa
       61 cgtcttgctg ctctggggaa aatttccagt actgagcaat ttctggaaga agtgtatcgc
      121 cgtgaaagcc ttggcccgac cgccttaggt gaagggttgg ctgtgccgca tggcaaaact
      181 gctgcggtaa aagaagcggc gtttgcggtc gccacactca gcgagccgct tcagtgggaa
      241 ggcgttgatg gcccggaagc ggttgattta gtggtgctgc tggcgattcc ccccaatgaa
      301 gcgggtacta cgcatatgca actgctgaca gcgctgacca cgcgcctggc ggatgatgag
      361 attcgggcgc gtatacagtc ggcgacgacg cctgatgagt tgctctcggc gctggatgac
      421 aagggaggca cgcaaccttc tgcctctttt tccaacgcgc caactatcgt ctgcgtaacg
      481 gcctgtccgg cgggtattgc tcacacctat atggctgcgg aatatctgga aaaagccgga
      541 cgcaaactcg gcgtaaatgt ttacgttgaa aaacaaggcg ctaacggcat tgaagggcgt
      601 ttaacggcgg atcaactcaa tagtgcaacc gcctgtattt ttgcggctga agtcgccatc
      661 aaggagagtg agcgttttaa tggcattccc gcgctttcag tgcctgttgc cgagccgatt
      721 cgccatgcag aagcgttgat ccaacaagcg cttaccctca agcgtagcga tgagacgcgt
      781 accgtacagc aagatacgca accggtgaaa agtgtcaaaa cggagctgaa acaggcactg
      841 ttgagcggaa tctcttttgc cgtaccgttg attgtcgcgg ggggcacggt gctggcggtc
      901 gcggtattac tgtcgcaaat cttcgggcta caagatctgt ttaatgaaga aaactcctgg
      961 ctgtggatgt accgcaagct gggcggcggg ctgctcggaa ttttgatggt accggtgctc
     1021 gcggcctata ccgcctattc tctggcagat aaaccggcgt tagcgccagg ctttgcggct
     1081 ggacttgccg ccaacatgat cggctccggg tttctcggcg cggtcgttgg cggattgata
     1141 gccggttact tgatgcgctg ggtgaaaaat cacttgcgtc ttagcagtaa attcaatgga
     1201 ttcctgactt tttatctcta cccggtgctc ggtacgttgg gagcgggcag tctgatgctg
     1261 tttgtggtgg gagaacctgt cgcctggatc aataactcgc ttaccgcctg gctgaacggt
     1321 ctgtcaggaa gtaacgcgct gttgctgggt gccattctcg gttttatgtg ttcctttgac
     1381 cttggagggc cagtgaacaa agccgcttat gcattctgcc tgggcgcaat ggcgaacggc
     1441 gtttacggcc cgtatgccat tttcgcctcc gtcaaaatgg tttcggcatt taccgtaacc
     1501 gcttccacga tgctcgcacc gcgcctgttt aaagagtttg aaattgagac cgggaaatcc
     1561 acctggctgt tagggctggc aggtattacc gaaggggcga tcccgatggc gattgaagat
     1621 ccgctgcggg ttattggttc gtttgtgctg ggctctatgg taatgggcgc tattgtcggt
     1681 gcgatgaata tcggcctttc gacacccggt gccggcattt tctcgctctt tttacttcat
     1741 gataatggcg cgggcggtgt tatggcggca attggctggt ttggtgcggc attggtgggg
     1801 gctgcaatct cgactgcaat tctcctgatc tggcggcgtc acgcggttaa gcatggcaac
     1861 tatctgactg atggcgtaat gccataaaca aaaaggaaac gacgatgaaa gcggtatctc
     1921 gcgttcacat caccccgcat atgcattggg atcgggagtg gtatttcacc accgaagagt
     1981 cacgcattct gctggtcaat aatatggaag agatcctgtg ccgactggaa caggacaacg
     2041 aatacaaata ttacgtactc gacgggcaaa cggcgatcct cgaagattat ttcgcggtga
     2101 aaccggaaaa caaagaccgc gtgaagaaac aggtagaagc cggcaagttg attatcggcc
     2161 cctggtatac ccagaccgat accacgattg tttctgcgga atccatcgtc cgtaatctga
     2221 tgtacggaat gcgtgactgc ctcgcgtttg gcgagccgat gaaaataggt tatttaccag
     2281 attcctttgg catgtccggg caactgccgc atatctacaa tggatttggc attacccgca
     2341 ccatgttctg gcgcggatgt tcggagcgcc acggtactga taaaaccgag tttttgtggc
     2401 aaagcagtga cggtagcgaa gtgacggcgc aggtgctgcc gctgggctac gccatcggta
     2461 agtacttacc tgccgacgaa aacggattac gtaaacgcct cgacagttat tttgacgtgc
     2521 tggaaaaagc gtctgtaacc aaagagattt tgctgccgaa tgggcatgac cagatgccat
     2581 tgcagcaaaa tatcttcgaa gtgatggata agctacgtga gatctaccct caacgtaagt
     2641 ttgtgatgag ccgctttgaa gaggtatttg agaagatcga agcgcagcgt gaaagtctgg
     2701 ccaccctgaa aggtgaattt atcgatggca aatatatgcg cgtacatcgc accatcggtt
     2761 ccacgcgcat ggatatcaaa attgcccacg cacgtattga aaataagatt gttaatctgc
     2821 tggaaccgct ggcaacgctg gcctggacgt tgggttttga ataccaccac ggcttgctgg
     2881 agaaaatgtg gaaagagatc ttaaaaaatc atgcccacga cagtatcggt tgttgttgca
     2941 gtgacaaagt tcatcgcgaa attgtcgccc gcttcgaact ggctgaagac atggcggata
     3001 atctgctgcg tttctacatg cgcaaaattg ccgataacat gccacagagt gatgccgaca
     3061 aactcgtgct gtttaacctg atgccctggc cgcgggaaga agttattaac accactgtgc
     3121 ggctgcgcgc cagccagttt aatctacgag acgatcgcgg tcagcctgta ccgtatttta
     3181 ttcgccatgc ccgtgagatc gatccaggcc taatcgatcg gcaaatagtt cattacggta
     3241 attacgatcc ctttatggag tttgatatac agatcaacca gattgtccct tctatgggct
     3301 atcgcacgct ttatatcgaa gcgaatcagc ctggcaacgt gattgcggca aaaagtgacg
     3361 ctgaagggat actggaaaat gctttctggc aaattgcgct caatgaggat ggaacgctac
     3421 aactggtaga taaagatagc ggtgtgcgct atgaccgggt gttgcaaatt gaagaaagct
     3481 ctgatgatgg tgatgaatat gactattcac ccgcaaaaga agagtgggta atgacttctg
     3541 caacagcgaa accgcaatgc gagattactc atgaggcctg gcagagcagg gctgttatcc
     3601 gctatgacat ggcggtgccg ctcaatttgt tggaacgaag cgtccggcaa tccacgggca
     3661 gagtaggggt ggagatggtt gtcactctga gtcataacag cagacgtatt gatgtggata
     3721 tcaaccttga taaccaggct gacgatcatc gccttcgcgt cctgatccct acatctttta
     3781 acaccgatag tgttctggca gatacccagt ttggttcgct aacgcgcccc gtgaacgaca
     3841 gtgcaatgaa caactggcag caagaaggct ggaaagaagc gccggttccg gtatggaata
     3901 tgctcaacta tgcagcctta caggaagggc gtaacagcat ggctgtcttt agcgaagggc
     3961 tacgtgaatt tgaagtcatc ggtgaagaga agaaaacctt tgccattacg ttgctgcgtg
     4021 gcgtgggctt actgggcaaa gaagatctgt ttttaaggcc tgggcggcct tcgggaatta
     4081 aaatgccagt cccggactca caactacgtg gtctgctttc ttgtcgccta agtttattga
     4141 gttataccgg tacgccaacc gccgctggtg tagctcagca ggcgcgagca tggctgactc
     4201 cagtacagtg ttacaacaaa atcccatggg atgcgatgaa gctcaacaaa gccggattca
     4261 acgtgccgga aagttatagt ttgttgaaaa tgcccccagt gggatgcctg ataagcgcac
     4321 ttaagaaagc tgaagaccga caagaagtga ttttacggct gtttaatccg gctgaatcag
     4381 caacctgtga tgcgactgtt gctttcagtc gcgaggtgat ttcttgctca gaaacgatga
     4441 tggatgaaca cattaccacc gaggaaaatc aaggttcaaa tctatcgggg ccttttttac
     4501 ccggccagtc acggacgttc agttaccggc ttgcctgaat agcaatcaaa ccgaagccac
     4561 atatgcgcgg ccagattgtt gacaaagggc gctttgttca tgccggatac ggcatgaacg
     4621 ctttattcgg tctacaaaag caggcaaatt caatatattg cagagatgat gtagacactg
     4681 gcaagcgtag cgcatcaggt aattttgcgt
//
LOCUS       DAZFQD010000227         4537 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.229, whole genome shotgun sequence.
ACCESSION   DAZFQD010000227 DAZFQD010000000
VERSION     DAZFQD010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4537
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.229"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..225
                     /locus_tag="ACHKTW_004967"
     CDS             <1..225
                     /locus_tag="ACHKTW_004967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO6465834.1"
                     /translation="HDWPPEIIKARLHMAGLSLRSLSLKAGYSRDSLKSVLRTPCRPY
                     QQIIADALGVSPEEIWPSRYQVKSYMRKAS"
     gene            227..2317
                     /locus_tag="ACHKTW_004968"
     CDS             227..2317
                     /locus_tag="ACHKTW_004968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu transposase C-terminal domain-containing
                     protein"
                     /protein_id="HFO6465835.1"
                     /translation="MYVIAKELIGAPGMPATTKGIRQALQRYVQGKSCCSRRRSGSKA
                     TEYSIDCLPEVTQQALRERYALQLMTQKADESPAPVVTKARRSPAVVDAVEAYRGSPQ
                     LMVERLNALTENQRKVAEARIAIVSEVLKIAQQPGFSCAKAIRFLVNSLARSQLDERI
                     VAMVETANAKKGNSRALSEITLKRWIAAFNKAQNAAERLLLLAPGKRQEIKAEDINWL
                     PEFLAQYRQSNGRPMTEAYEDFVAEWQHRHADEPYMLDIMPSYDTIRRAMKKLPEVVK
                     QKGRVTGSEYRQLEGFTRRDWSRMPVNYVWIGDGHGMKLKCAHPVHGRPFAPEVTFVI
                     DGGTRFVVGWSLDLAENVFAVAGAIQHGIRHHGKPFLYYSDNGSGETADVLDKEIVGI
                     LPRLGINHPTGIAGNPQGRGIIERLNRTLPMRIARKYRTYFGKGADRETLRKTNRDLR
                     SAFSALQQGKRLNARQQSAMRDLPSWSELIDAIRDGVEWYNNRPHDELPMKPNGKHYS
                     PAEFRKKRLAEEDTEIEWLSDVELRDMFRPMVERPVRRCEIRWLNNIYYAPELRDEHG
                     RKVLISYDIHDAERITVRRLDGSVICEAVWDGNKREAFPVSAEYYKQQQRLKGMRKRA
                     EEKIRDAEDEVINVLEHKPQEPWLENIYRPMGNTVAVQQPAIDDEPDEEYERNFQRGL
                     QLLEAKLKENDPLA"
     gene            2399..3331
                     /locus_tag="ACHKTW_004969"
     CDS             2399..3331
                     /locus_tag="ACHKTW_004969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFO6465836.1"
                     /translation="MTDINDVIKTIDELIDGGVLTQYAIAREAGISDGTLSAFRKGKY
                     KGDNDAITAAMLSWYENWNKQSALSELPQFVETQTVQELRALFQAVRLMGCINVIVGV
                     PGVGKTATARNYCQEQPNTWMITLSPAHSSVTECLLELAEELGIDYTRANKGALSRAI
                     RRRLMGKRGLVIVDEADHLGIDGLEQLRAIQDATGVGMVLIGNPKGLFKGGRRAVDDL
                     SRLFSRLARTKQLRKAKKADVLAIARAWGISGEAELAVMQAIAEKPGALRVLTHTLNQ
                     AWITASGEGAALTEKHINAAFKEVYANPELLSQV"
     gene            3334..3555
                     /locus_tag="ACHKTW_004970"
     CDS             3334..3555
                     /locus_tag="ACHKTW_004970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6465837.1"
                     /translation="MIAFNIPDIYGRFHLVNFDNVKAVSVSDSEECGDLLFEFNDRTR
                     MAISAGLDREGAKEAYSNICRFIGAKRIS"
     gene            3973..4200
                     /locus_tag="ACHKTW_004971"
     CDS             3973..4200
                     /locus_tag="ACHKTW_004971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001047891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6465838.1"
                     /translation="MNLFDSLNNARRLTELAGAVLERSKRYPQRFALKTTPPVNEVKG
                     TGEIEITVKTNGLRRRVKATRISGCTVYWEV"
     gene            4275..>4537
                     /locus_tag="ACHKTW_004972"
     CDS             4275..>4537
                     /locus_tag="ACHKTW_004972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6465839.1"
                     /translation="MSKVVRIIFEYKEHVIHKNADGTVRMGVSLDIRSTGIKQKGDGP
                     AMIFWVVMLAESRYFAELVAMKASALMKDMSMRSGVIKGNEFN"
BASE COUNT         1200 a         1056 c         1267 g         1014 t
ORIGIN      
        1 cacgattggc cgcctgaaat catcaaggcg cgcttacaca tggctggctt atcactgcgc
       61 tctttatctt taaaggctgg ttacagcagg gattcgctga aaagcgtatt acgtactcct
      121 tgccgaccgt atcagcaaat aattgctgat gctcttgggg tatcacctga agaaatctgg
      181 cccagtcgat accaggttaa gagctatatg agaaaggcgt catgatatgt atgtcattgc
      241 aaaagaactg attggcgcgc ccggaatgcc tgctacaaca aaaggtattc gccaggcatt
      301 acaacgttac gtacaaggga aaagctgttg ttcccgtcgt cgctcaggct ctaaagcaac
      361 tgaatacagc atcgactgtt tacctgaagt gacgcagcag gcattacgtg aacgctacgc
      421 cctgcaactg atgacgcaaa aagccgatga atcaccggct ccggtggtga caaaggccag
      481 acgctcacct gccgtggttg atgcggtgga ggcatatcgc ggatcacccc aactgatggt
      541 cgaacgcctc aatgccctga ctgaaaacca acgtaaagtg gcagaggcac gaatagcgat
      601 cgtcagcgag gtgctgaaaa tcgcgcaaca acccggcttt agttgcgcta aggctatccg
      661 gttcctcgtt aacagcctgg cacgatcaca actggacgag cgcattgtgg caatggttga
      721 gacggcgaac gccaaaaagg gaaacagccg cgcgttgagt gaaatcacgc tgaaacgctg
      781 gattgcggcc tttaacaagg cacagaacgc cgctgaacgc ctgcttttac tggcaccggg
      841 taaacgccag gaaataaaag ccgaagatat taactggctg cccgaatttc tggcgcagta
      901 tcgccagtca aacggccgac caatgaccga ggcttacgag gattttgttg ctgaatggca
      961 gcaccggcac gctgatgagc cttatatgct cgatatcatg ccctcttatg acaccattcg
     1021 ccgcgcaatg aagaaactgc cggaagtggt gaaacaaaaa ggccgggtaa ctggcagtga
     1081 ataccgtcag cttgagggat tcacgcgccg cgactggtcc agaatgccgg tgaattatgt
     1141 ctggattggt gacggtcacg gcatgaagct gaaatgcgcg cacccggttc acgggcggcc
     1201 attcgcaccg gaagtgacct ttgttatcga cggtggcacg cgctttgtgg tgggctggag
     1261 ccttgacctg gctgaaaatg ttttcgccgt agccggtgcc atacagcacg gtattcgcca
     1321 tcatggcaaa ccgtttctgt attactcgga taatggctcc ggggaaaccg ccgacgtcct
     1381 ggataaggag attgtgggga tactgccacg actggggatt aatcacccga cagggattgc
     1441 cggtaatccg cagggacggg gcattatcga acggcttaac cgcacattac cgatgcgcat
     1501 agcccgtaaa taccgcacct atttcgggaa aggagcagat cgcgagacgt tacgtaaaac
     1561 caaccgcgat ttacgctcgg cattcagtgc cctgcaacag ggcaaacggc tgaacgcccg
     1621 gcagcagtca gcgatgcgtg atttaccgtc ctggtctgaa ctgattgatg ccattcgtga
     1681 cggtgttgag tggtacaaca accggccgca cgatgaatta cccatgaagc cgaacggtaa
     1741 acattacagc ccggcggagt tcagaaaaaa acgcctggcg gaagaggaca cggaaattga
     1801 atggctgtcc gatgtggaat tgcgggacat gttccggccg atggtggaac gccctgtaag
     1861 acgctgtgaa atacgctggc tgaataatat ttactacgcg cccgagctgc gtgatgaaca
     1921 tggccgcaaa gtgcttatca gctatgacat tcatgatgcc gaacgaatta ccgtgcgtcg
     1981 cctggatggc agcgtgattt gcgaggcggt atgggacggc aataaacgcg aagccttccc
     2041 ggtcagcgcg gaatactaca aacagcagca gcgtcttaaa ggcatgcgta aacgcgcaga
     2101 ggaaaaaatt cgtgatgccg aggatgaggt tatcaacgtg ctggagcaca agccgcagga
     2161 gccatggctg gaaaacatat accgccctat gggtaatacg gtggccgttc agcagccagc
     2221 gattgatgat gaacctgacg aagaatacga gcgtaatttc cagcggggat tacagctgct
     2281 tgaagcgaaa ttaaaagaaa atgacccgct ggcctgaaat aaaaaaataa cccgaggggt
     2341 aactcaggtt atttgggttt cagtctcgga gaatgctgtc tcaagcgagg taaataatat
     2401 gactgatatt aacgatgtaa tcaagaccat agatgaactt attgatggcg gcgtactgac
     2461 gcagtatgcc atcgccaggg aggcgggaat ttccgacggc acattatcgg ctttccgcaa
     2521 agggaaatat aaaggagaca atgatgctat aacggcggcc atgctttcct ggtatgaaaa
     2581 ctggaataaa caaagcgcac tgtccgagct gccacagttt gtggaaacgc agacagtcca
     2641 ggagctgcgc gcactgtttc aggcggttcg cctgatgggc tgtattaacg tcatagtggg
     2701 cgtgccgggt gtgggtaaaa cggccaccgc ccgtaattac tgccaggagc aaccgaacac
     2761 ctggatgatc accctgtcac ccgcgcactc cagcgtcacg gagtgtctgc tggagctggc
     2821 agaggaactg gggattgatt acacccgcgc gaacaaaggg gcattatccc gcgccatccg
     2881 ccgtcgcctg atgggaaagc gcggactggt gattgttgat gaggctgatc atcttggtat
     2941 tgacggtctg gagcaactcc gggcaatcca ggacgccaca ggggtcggga tggtgcttat
     3001 tggtaaccca aaggggctgt ttaaaggcgg acgccgcgcc gttgatgatt tatctcgcct
     3061 gttcagccgt cttgcccgta caaaacaact acgcaaggcc aaaaaagcgg atgtgctggc
     3121 tattgccagg gcatggggga ttagtggtga ggccgagctg gctgtcatgc aggctatcgc
     3181 tgaaaaaccg ggggcattac gcgttctgac gcatacgctt aaccaggcgt ggatcaccgc
     3241 cagcggtgaa ggtgcggcac tgacagaaaa acatattaat gcggccttta aagaggttta
     3301 tgccaaccct gaattactct cacaggtgtg attatgattg catttaatat tcctgatatt
     3361 tacggacgct ttcacctggt gaatttcgat aacgtcaaag cagtctccgt gtctgacagt
     3421 gaagaatgtg gcgatttact ttttgaattt aatgatcgca ccagaatggc aatatcggca
     3481 ggacttgatc gtgaaggtgc gaaagaagct tacagcaata tttgtcgttt tatcggtgca
     3541 aaacgaatca gctaaacgag gtgttatatg aatacgcaga tacagcagga aatcaatgtt
     3601 ctccacagct ttcgagagtt taacgacaat ggctgtgagg ttcgtgatta tatctatgaa
     3661 ttgatgatcg gtgatggtgt gtgttttctg tatatagacc tgtatcagtg gtatgcagac
     3721 atgcaacttg attccatcag acgccgtttt aatcatgaat gctttgatgt ggatgtatgg
     3781 gaaagcctgc gctttggttt tctgattatg gatgattatc gccaggggtg gcgacacggt
     3841 tactccattc ttcgtgcaat catgcgtggc gaaacccgaa aaattccggg gatgggtaaa
     3901 gttaatcgag gcttcctgtc agagctggct gcattgcgca tgaatccatt actgatacaa
     3961 ccggagttcc ctatgaattt gtttgattca ctgaataacg cccgccgcct gactgaactt
     4021 gctggtgcgg tactggagcg cagcaaacgc tacccgcaac gttttgcact gaaaaccacg
     4081 ccgccggtaa acgaagttaa gggaaccggt gaaattgaaa tcaccgttaa gaccaacggc
     4141 ctgcgccgcc gtgtgaaggc cacccgtata agcggatgca cggtttactg ggaagtatga
     4201 agttgcttca tggttcggct ctgtcatcag gtcagacgcc gggtcagtta tccgaacaaa
     4261 acgaggacca gaggatgagt aaagtcgtac gcattatttt cgaatacaag gagcacgtta
     4321 tccataaaaa cgcggatgga acagtgcgca tgggggtaag tctggacata cgttcaaccg
     4381 ggataaagca gaaaggtgat ggacctgcca tgattttttg ggtggttatg ctcgcggaaa
     4441 gcagatactt tgctgaactt gtggcaatga aagccagtgc gctcatgaaa gatatgagca
     4501 tgcgttccgg ggttattaaa ggtaatgaat ttaatca
//
LOCUS       DAZFQD010000228         4526 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.230, whole genome shotgun sequence.
ACCESSION   DAZFQD010000228 DAZFQD010000000
VERSION     DAZFQD010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4526
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.230"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..338)
                     /locus_tag="ACHKTW_004973"
     CDS             complement(<1..338)
                     /locus_tag="ACHKTW_004973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000547696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO6465840.1"
                     /translation="MHIKNFRQYTPENPDVPGAMYLKSEDGQDWYECQSLFSAETLKV
                     VYNSAGVITGISRVASVLWPVNQSVVEVADTEENRKADISGRWGFDGEKITDLLTAEK
                     ARGMKGDDINA"
     gene            complement(380..2107)
                     /locus_tag="ACHKTW_004974"
     CDS             complement(380..2107)
                     /locus_tag="ACHKTW_004974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFO6465841.1"
                     /translation="MTVKISGVLKDGAGKPVPGCTIELKARRTTETVIVTTVAYGQPG
                     ETGSYSMDVEPGLYRVTLNTEGYAPSYVGDILVKADSAPGTLNKFLMDLEDAQYYPKA
                     LAELEAVAAEILKRAEASAASAEEAKKRAENARGPKGDKGDTGPQGIPGPKGDTGERG
                     PKGERGERGPQGLQGVKGERGEKGEKGEPGGPDATTAQKGIVQLSSATDSDDETKAAT
                     PKAVKAAMDKADGCLEKAKNGDDIPDKVQFLNTVGAARVYGRDIHTGAGEWTTGEFVA
                     WLKAKGAFDQPYWMMKASLHAGVNKVITDVGPGKLNLGGCVIEVMGKYEAAIVRVTIG
                     EYGATGFINGTVCTCTVYGDTQYFHWRVDYSTKNKPDTVSQRDASTTQKGVVQLSSDT
                     NSNDETKAATPKAVKAAMDVANEAKTKAEEAAAGGGVPGPKGEKGDPGAQGPKGETGA
                     TGPAGPRGAQGPKGEKGDPGPRGERGETGPMGPPGASDGKSRVVGIRLGNKQTYAPQT
                     DSTTWAVDLDIGAMITGIGGYNDGNKTLIDRVSYRPLQVTFDGSQWRTVSVGEYVSSG
                     VSGFEYFPL"
     gene            complement(2257..2880)
                     /locus_tag="ACHKTW_004975"
     CDS             complement(2257..2880)
                     /locus_tag="ACHKTW_004975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001408020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFO6465842.1"
                     /translation="MRYLYGAILFFTTLPAVMTFPAQAAGGHGAFSVGYAQVHPGGVP
                     VLSGTGARTGDLKGINVKYRYEFTDHLGGIASLSYASAKKSRTTMTGDKAFHYESLRG
                     RYVSLMAGPVWQVSEQLSLYGMAGMAHTRWSDSVQDYRRDEVTQGDVRVTTTASDGHS
                     ARHLTLAWGAGLQFNPADTVVVDLAYEVAGHGDWRTDAFIVGIGYRF"
     gene            complement(2949..>4526)
                     /locus_tag="ACHKTW_004976"
     CDS             complement(2949..>4526)
                     /locus_tag="ACHKTW_004976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003955761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HFO6465843.1"
                     /translation="FRINAPAAPASIELTPGYFQITVVPHPAVYDPTVQYEFWFSEKR
                     ITDTAQVETSARYLGTGSQWSVSGPHIKPGKDFWFYVRSVNLVGKSAFVEASGQASND
                     GEGYLEIFRGLIDEALLGKALKERIDASALRTEVTQLEEDIRQRLETDVAEVTRKIGE
                     AENSLTQLVAKKNEDQTLAIAQVSQQVDRVSSEITQTVSQSTEENARQIAQVRQYVDD
                     KGSEITSTTDKKLEDQSATIQQIQQVQSDTNDELAALYMLKVQKTKNGIPYVAGIGAG
                     IEDADGQTLSNILLQADRIAMITPENGNTTPLFVAQGNQLFMNDVFLKRLFAVSITSS
                     GNPPTFSLTPEGRLTARNADISGHISANSGTLNNVTINENCVIRGKLSANQIEGDLVK
                     TVGKAFPRNNSYARGTITVTVYDDQGFDRQIIIPPVLFRGTKHQNVNSPNQQSYWYST
                     CKLQVLKNGAEIFHEPATDVSRVFSSVIDMPAGRGHVTLTFNVSSAGANNWTPTTYIS
                     DLLVVVMKKSTAGISIS"
BASE COUNT          883 a         1489 c         1009 g         1145 t
ORIGIN      
        1 gcgttaatgt catcgccctt catccctcgc gctttctccg cggtcagcag gtccgtgatt
       61 ttctccccgt caaaacccca gcgccccgag atgtcagctt tgcggttttc ttccgtatcc
      121 gccacctcca ccacactctg gttcacaggc cacagaactg acgccaccct gctaatgccg
      181 gtaatgaccc cggcactgtt ataaaccacc ttcagcgtct ctgctgaaaa taacgactga
      241 cactcatacc agtcctgacc atcctctgat ttcaggtaca tcgcacccgg cacatccgga
      301 ttttccggcg tgtattgtct gaaattcttt atgtgcatct gtaagtcctc ccggttacat
      361 gtctgaaaga tgatgcgctt tataatggaa aatactcaaa ccccgaaact cctgacgaca
      421 catactcacc cacgctgact gtacgccact gagaaccgtc aaatgtcacc tgaagcggcc
      481 tgtaagatac cctgtcaata agggttttgt ttccgtcgtt gtaaccgcca atccccgtaa
      541 tcatcgcacc aatatccaaa tccactgccc atgtagtgct gtctgtttgc ggcgcataag
      601 tctgtttatt acccagcctg atacccacaa cccggctctt cccgtctgat gccccgggcg
      661 gccccatcgg cccggtttct ccacgctcac ctctgggacc ggggtcgcct ttctcccctt
      721 tcggtccctg cgctccccgc ggaccagcgg gacctgttgc tcccgtttca cctttcggac
      781 cctgtgctcc ggggtcgcct ttctctcctt tcggaccggg aacaccacct cctgcagcag
      841 cctcttctgc ctttgttttc gcttcatttg ccacatccat tgccgccttc acggccttcg
      901 gcgtggctgc ctttgtttcg tcattactgt tagtatcact gcttaactgc accacgcctt
      961 tctgcgtcgt gctggcatcc cgctggctga ccgtatccgg tttgttcttt gtgctgtaat
     1021 ccacccgcca gtggaaatac tgtgtgtcgc cgtaaaccgt acaggtacag accgtgccat
     1081 taataaaccc cgttgcaccg tattcgccaa tggtgacccg gactatggct gcctcatact
     1141 tccccatcac ctcaatgaca caaccaccca gattaagttt tcccggtccg acatccgtga
     1201 ttaccttatt gactcccgca tgcagtgatg ccttcatcat ccagtaaggc tggtcaaatg
     1261 cccctttggc tttcagccag gcaacaaact cgcccgtggt ccattcaccg gcccccgtat
     1321 gaatgtcccg cccgtatact ctggctgctc ccacagtgtt cagaaactgc accttatccg
     1381 gaatatcgtc accgtttttc gctttctcaa ggcatccgtc cgctttgtcc attgccgctt
     1441 tcaccgcttt cggggtggct gccttcgttt catcatcact gtccgttgcg ctgcttaact
     1501 gcacaatgcc cttctgtgcc gtcgtcgcat ccggtcctcc cggctcgcct ttttcgccct
     1561 tctctccccg ttcacctttc acaccctgaa gcccctgcgg ccccctctca ccacgctcac
     1621 cctttggccc acgctcgccg gtatcgcctt ttggcccggg aataccctgt ggcccggtgt
     1681 ctcccttatc ccccttcggt ccccgcgcat tctctgcccg tttcttcgct tcctctgcac
     1741 tcgccgctga cgcttctgca cgtttcagga tttccgcggc caccgcttcc agctctgcaa
     1801 gggctttcgg gtaatactgt gcgtcctcca ggtccatcag aaatttattc agcgttcccg
     1861 gtgcagagtc cgccttcacc agaatgtcac ccacatatga cggcgcgtac ccttccgtgt
     1921 tcagcgtcac ccggtacaac cccggctcaa catccatact gtaactgccg gtttcccccg
     1981 gctgaccata cgccaccgtg gtgacaatca ccgtctccgt tgtgcgtcgc gctttcagct
     2041 ctatcgtgca tcccggtacc ggcttaccgg ccccgtcttt caatacacca gaaattttaa
     2101 ccgtcatact tttccaccaa taaaaaagcc cgcagcagta atgccacggg cttcaggaca
     2161 gtgtaacttt acgtttcctc agacgcagtt caccccataa ggcggatgaa catgcgtaca
     2221 ttgcaatatt tgcagaaaat aaagcagcat ctgccgtcag aaacggtatc cgatcccaac
     2281 aataaacgca tccgttcgcc agtcaccatg accggccact tcgtacgcaa ggtcaaccac
     2341 caccgtatcc gccggattaa actgaagccc tgccccccat gccagcgtca gatgacgcgc
     2401 agaatgacca tcactggcag tggtggtcac cctgacatcc ccctgtgtca cttcatcacg
     2461 ccggtaatcc tgaacactgt cagaccagcg ggtgtgcgcc atcccggcca tgccataaag
     2521 gctgagctgc tcactgacct gccagaccgg ccccgccatc aggctcacat aacggccacg
     2581 cagactttca taatgaaatg ctttatcccc ggtcatcgtg gttctgctct ttttcgcaga
     2641 ggcgtaactc agcgaggcta tgccacccag atggtccgtg aattcataac ggtatttcac
     2701 gttgatccct tttaaatcac ctgtacgggc accggtaccg gacaataccg gcacgccgcc
     2761 cgggtgaacc tgagcatatc ccacggaaaa tgcaccgtgt ccgcctgcag cctgtgcagg
     2821 aaaagtcatt actgccggca gggtggtaaa aaataaaatg gctccgtata aataccgcat
     2881 gattacctct ttgttttcag tcaataaaaa aggcaccact tcgggtgccc gtccgggtta
     2941 ataaaccgtc agctgatact gattcccgcc gtggattttt tcatcaccac aaccagtaaa
     3001 tcactgatgt acgtcgtcgg tgtccagttg ttcgcgccgg cagacgacac attaaacgtc
     3061 agggtgacat gaccccgtcc tgctggcatg tcaatcaccg atgagaacac ccggctgaca
     3121 tccgttgccg gttcatgaaa aatctctgcc ccgtttttca gcacctgcag cttacaggtt
     3181 gaataccagt acgactgctg attcgggctg ttgacgttct ggtgtttcgt cccgcgaaac
     3241 agtaccggtg gaatgataat ctgccggtcg aagccctggt catcgtaaac cgtgacggtt
     3301 atcgtgccac gggcataact gttattccgg ggaaaggctt tccccaccgt cttcaccaga
     3361 tcgccttcaa tctggttcgc agacagtttc cctctgatga cacagttctc gttaatggtg
     3421 acattattga gcgtgcccga gttcgcactg atatgtccgc tgatgtccgc attgcgggct
     3481 gtcagcctgc cctccggcgt cagggaaaac gtcgggggat tgccggatga cgtgatactc
     3541 accgcaaaca gtcgcttcag gaacacgtcg ttcataaaca gctgattccc ctgcgccaca
     3601 aacagcggcg tggtgttgcc gttctccggg gtaatcatcg cgatacggtc cgcctgcagc
     3661 agaatattgc tcagcgtctg gccatcagca tcctcaatcc ccgcaccaat accggcaaca
     3721 tacggaatgc cgtttttcgt tttctgcacc ttcagcatgt acagcgctgc cagctcatca
     3781 ttcgtgtctg actggacctg ctgtatctgc tgtatggtgg cgctctggtc ttccagcttt
     3841 ttatccgtgg tcgaggtgat ttcactccct ttgtcatcca cgtactggcg gacctgcgct
     3901 atctgcctgg cgttttcctc cgtgctctgg ctgactgtct gtgtgatttc actgctcacc
     3961 cggtccacct gctgactcac ctgcgcgatg gccagcgtct ggtcctcatt ctttttcgca
     4021 accagctgcg tgaggctgtt ttccgcctcc ccgatttttc gggtcacttc cgcaacatcc
     4081 gtctccagtc gctggcggat gtcttcttcc agttgcgtga cctcagtacg cagggctgac
     4141 gcatcaatac gctctttcag tgccttcccc agcagcgcct catctatcag cccacggaaa
     4201 atttccagat acccttcacc atcattgctg gcctgcccgc tggcctccac aaacgcagat
     4261 ttccccacca ggttgacgct gcgcacataa aaccagaaat ccttccccgg cttaatgtgc
     4321 gggccggaga cgctccactg gctgccggta cccagataac gggcagaggt ttccacctgt
     4381 gccgtgtccg tgatgcgttt ttctgagaac cagaattcat actgtaccgt cgggtcatac
     4441 accgcaggat gcgggaccac cgttatctga aaatagcccg gcgtcagttc aatgctggcc
     4501 ggtgccgccg gtgcattaat ccggaa
//
LOCUS       DAZFQD010000229         4454 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.231, whole genome shotgun sequence.
ACCESSION   DAZFQD010000229 DAZFQD010000000
VERSION     DAZFQD010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4454
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.231"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            331..2289
                     /gene="acs"
                     /locus_tag="ACHKTW_004977"
     CDS             331..2289
                     /gene="acs"
                     /locus_tag="ACHKTW_004977"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HFO6465844.1"
                     /translation="MSQIHKHTIPANIADRCLINPQQYEAMYQQSINVPDTFWGEQGK
                     ILDWIKPYQKVKNTSFAPGNVSIKWYEDGTLNLAANCLDRHLQENGDRTAIIWEGDDA
                     SQSKHISYKELHRDVCRFANTLLELGIKKGDVVAIYMPMVPEAAVAMLACARIGAVHS
                     VIFGGFSPEAVAGRIIDSNSRLVITSDEGVRAGRSIPLKKNVDDALKNPNVTSVEHVV
                     VLKRTGGKIDWQEGRDLWWHDLVEQASDQHQAEEMNAEDPLFILYTSGSTGKPKGVLH
                     TTGGYLVYAALTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGATTLMFEGVP
                     NWPTPARMAQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRSSLRILGSVGEPINP
                     EAWEWYWKKIGNEKCPVVDTWWQTETGGFMITPLPGATELKAGSATRPFFGVQPALVD
                     NEGNPLEGATEGSLVITDSWPGQARTLFGDHERFEQTYFSTFKNMYFSGDGARRDEDG
                     YYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHNIKGQAIYAYVTLN
                     HGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILRKIAAGDTS
                     NLGDTSTLADPGVVEKLLEEKQAIAMPS"
     gene            2489..2803
                     /locus_tag="ACHKTW_004978"
     CDS             2489..2803
                     /locus_tag="ACHKTW_004978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HFO6465845.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAIYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPIGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            2800..4449
                     /gene="actP"
                     /locus_tag="ACHKTW_004979"
     CDS             2800..4449
                     /gene="actP"
                     /locus_tag="ACHKTW_004979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015123 - acetate transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HFO6465846.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRSDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTFADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVAGLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMMGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSISVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
BASE COUNT          983 a         1174 c         1230 g         1067 t
ORIGIN      
        1 cggtatcaaa tgaaatgcaa atcggtatca aaaaaatcgc cgcgctataa accacactgt
       61 gaatgttgtc tttaatcaat tgtaagtgca tgtaaaatac cactttagag ttagtcagta
      121 tcttcctctt tttcaacagc atgcataact gcatgttcct caaagaatta atcaactttt
      181 gttgctgacc ttcaaaaatt accctgccgt ttatttgcac aattctactt ttgcgtgatc
      241 tgtcgcccaa atactaaaca aaactgccaa tacccctaca tttaacgctt atgccacata
      301 ttattaacat cctacaagga gaacaaaagc atgagccaaa ttcacaaaca caccattcct
      361 gccaacatcg cagaccgttg cctgataaac cctcagcagt acgaggcgat gtatcaacaa
      421 tctattaacg tacctgatac cttctggggc gaacagggaa aaattcttga ctggatcaaa
      481 ccttaccaga aggtgaaaaa cacctccttt gcccccggta atgtgtccat taaatggtac
      541 gaggacggca cgctgaatct ggcggcaaac tgccttgacc gccatctgca agaaaacggc
      601 gatcgtaccg ccatcatctg ggaaggcgac gacgccagcc agagcaaaca tatcagctat
      661 aaagagctgc accgcgacgt ctgccgcttc gccaataccc tgctcgagct gggcattaaa
      721 aaaggtgatg tggtggcgat ttatatgccg atggtgccgg aagccgcggt tgcgatgctg
      781 gcctgcgccc gcattggcgc ggtgcattcg gtgattttcg gcggcttctc gccggaagcc
      841 gttgccgggc gcattattga ttccaactca cgactggtga tcacttccga cgaaggtgtg
      901 cgtgccgggc gcagtattcc gctgaagaaa aacgttgatg acgcgctgaa aaacccgaac
      961 gtcaccagcg tagagcatgt ggtggtactg aagcgtactg gcgggaaaat tgactggcag
     1021 gaagggcgcg acctgtggtg gcacgacctg gttgagcaag cgagcgatca gcaccaggcg
     1081 gaagagatga acgccgaaga tccgctgttt attctctaca cctccggttc taccggtaag
     1141 ccaaaaggtg tgctgcatac taccggcggt tatctggtgt acgcggcgct gacctttaaa
     1201 tatgtctttg attatcatcc gggtgatatc tactggtgca ccgccgatgt gggctgggtg
     1261 accggacaca gttacttgct gtacggcccg ctggcctgcg gtgcgaccac gctgatgttt
     1321 gaaggcgtac ccaactggcc gacgcctgcc cgtatggcgc aggtggtgga caagcatcag
     1381 gtcaatattc tctataccgc acccacggcg atccgcgcgc tgatggcgga aggcgataaa
     1441 gcgatcgagg gtaccgaccg ttcgtcgctg cgcattctcg gttccgtggg cgagccaatt
     1501 aacccggaag cgtgggagtg gtactggaaa aaaatcggca acgagaaatg tccggtggtc
     1561 gatacctggt ggcagaccga aactggcggt ttcatgatca cgccgctgcc tggcgctacc
     1621 gagctgaaag ccggttcggc aacacgtccg ttcttcggcg tgcaaccggc gctggtcgat
     1681 aacgaaggta acccgctgga aggcgctacc gaaggcagcc tggtgatcac cgactcctgg
     1741 ccgggtcagg cgcgtacgct gtttggcgat cacgaacgtt ttgaacagac ctacttctcc
     1801 accttcaaaa atatgtattt cagcggcgac ggcgcgcgtc gcgatgaaga tggctattac
     1861 tggatcaccg ggcgtgtgga cgacgtgctg aacgtctccg gtcaccgtct ggggacggca
     1921 gagattgagt cggcgctggt ggcgcatccg aagattgccg aagccgccgt agtaggtatt
     1981 ccgcacaata ttaaaggtca ggcgatctac gcctacgtca cgcttaatca cggggaggaa
     2041 ccgtcaccag aactgtacgc agaagtccgc aactgggtgc gtaaagagat tggcccgctg
     2101 gcgacgccag acgtgctgca ctggaccgac tccctgccta aaacccgctc cggcaaaatt
     2161 atgcgccgta ttctgcgcaa aattgcggcg ggcgatacca gcaacctggg cgatacctcg
     2221 acgcttgccg atcctggcgt agtcgagaag ctgcttgaag agaagcaggc tatcgcgatg
     2281 ccatcgtaac ccacaattgc cggatgcgag tcggtaacgg tttgtaggcc tgataagacg
     2341 cgacagcgtc gcatcaggca ttgattgccg gatgcggcgt aaacgcctta tccggcctac
     2401 attcggcaag ggttacccga gcgttaacct tctcccataa gggagcggga attaaaacaa
     2461 tccctacatt acctctggag aatctgtgat gaatggcact atttatcagc ggatagaaga
     2521 caatgcgcat ttcagggagt tagtcgaaaa acggcaacgg tttgccacca tcctgtcgat
     2581 tattatgctg gcaatttata tcggctttat tttactgatc gccttcgcgc ccggctggct
     2641 gggcacgccg ctgaatccga acaccagcgt cacacgcggt attccaattg gtgttggagt
     2701 gattgtgatc tcctttgttc tcaccggtat ctacatctgg cgggcgaacg gcgaattcga
     2761 ccgtcttaat aatgaagtcc tgcatgaggt acaagcatca tgaaaagagt tctgacggcg
     2821 cttgccgcca cactcccttt cgcagctaac gccgcggatg ctattagcgg ggccgtagag
     2881 cgccagccaa cgaactggca ggcgattatt atgttcctga ttttcgtcgt gtttacgctc
     2941 ggcattacct actgggcatc aaaacgcgta cgttctcgta gcgactacta caccgcaggc
     3001 ggcaatatca ctggcttcca gaacgggctg gcgattgccg gggactatat gtccgccgcc
     3061 tcattcttgg ggatctccgc gctggtgttt acctccggct atgacggctt aatttactcg
     3121 ctgggcttcc tggtgggctg gccgatcatt ttgttcctga ttgccgaacg tctgcgtaac
     3181 ctggggcgct acacctttgc cgatgtggcc tcttaccgtc tgaaacaagg gccgattcgt
     3241 attctttcgg cctgtggttc tctggtggtg gtggcgcttt accttatcgc ccagatggtg
     3301 ggcgcaggta aactgatcga gctgctgttt ggccttaact atcacattgc ggtggtgctg
     3361 gtcggcgtgc tgatgatgat gtacgtcctg ttcggcggca tgctggcgac cacttgggta
     3421 cagattatca aagccgtgct gttgctgttc ggtgccagct ttatggcctt tatggtgatg
     3481 aaacacgtcg gctttagctt caacaatctg ttcagtgaag cgatggcggt acacccgaaa
     3541 ggtgtcgaca tcatgaagcc gggcgggctg gtgaaagatc cgatctccgc gctctctctg
     3601 ggtctgggac tgatgtttgg tacggcgggc ttgccgcaca ttctgatgcg cttctttaca
     3661 gtcagcgatg cccgcgaagc acgtaagagc gtgttctacg ccaccgggtt tatgggctac
     3721 ttctatattc tgacctttat tatcggcttc ggcgcgatca tgctggttgg tgcgaatccg
     3781 gaatataaag acgcggcggg ccatctgatt ggtggtaaca acatggcggc cgttcacctg
     3841 gcgaatgcag tgggcggcaa cctgttcctc ggttttattt cagcggttgc ttttgccacc
     3901 attcttgcgg tggttgctgg tctgacgctg gcgggcgcat cagcggtttc gcatgacctg
     3961 tacgctaacg tcttcaaaaa aggcgcgacc gaacgtgaag aactgcgggt atcgaaaatc
     4021 accgtgctga ttttaggtgt gattgccatt atcctcggcg tgctgtttga gaatcagaac
     4081 atcgccttca tggtaggcct ggcgtttgcc atcgcagcga gctgtaactt cccgatcatt
     4141 ctgctttcta tgtactggtc gaaactgacc actcgtggcg cgatgatggg tggctggctg
     4201 gggctgatta ccgcagtggt actgatgatc ctcggcccga cgatttgggt acagatcctt
     4261 ggtcacgaaa aagccatctt cccgtatgaa tacccggcgc tgttctctat cagcgtggca
     4321 ttcctcggca tctggttctt ctcggcaacc gataactcag cggaaggtgc gcgcgagcgt
     4381 gaactgttcc gtgcacagtt tatccgctcc cagaccggct ttggcgttga gcaaggccgc
     4441 gcacattaat ctct
//
LOCUS       DAZFQD010000230         4390 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.232, whole genome shotgun sequence.
ACCESSION   DAZFQD010000230 DAZFQD010000000
VERSION     DAZFQD010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4390
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.232"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..209)
                     /locus_tag="ACHKTW_004980"
     CDS             complement(<1..209)
                     /locus_tag="ACHKTW_004980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFO6465847.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(324..399)
                     /locus_tag="ACHKTW_004981"
     tRNA            complement(324..399)
                     /locus_tag="ACHKTW_004981"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(364..366),aa:Thr,seq:ggt)
     gene            complement(406..480)
                     /locus_tag="ACHKTW_004982"
     tRNA            complement(406..480)
                     /locus_tag="ACHKTW_004982"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(445..447),aa:Gly,seq:tcc)
     gene            complement(597..681)
                     /locus_tag="ACHKTW_004983"
     tRNA            complement(597..681)
                     /locus_tag="ACHKTW_004983"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(645..647),aa:Tyr,seq:gta)
     gene            complement(690..765)
                     /locus_tag="ACHKTW_004984"
     tRNA            complement(690..765)
                     /locus_tag="ACHKTW_004984"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(730..732),aa:Thr,seq:tgt)
     gene            1127..2077
                     /gene="coaA"
                     /locus_tag="ACHKTW_004985"
     CDS             1127..2077
                     /gene="coaA"
                     /locus_tag="ACHKTW_004985"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFO6465848.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHAVEEVRLRK"
     gene            complement(2106..3071)
                     /gene="birA"
                     /locus_tag="ACHKTW_004986"
     CDS             complement(2106..3071)
                     /gene="birA"
                     /locus_tag="ACHKTW_004986"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HFO6465849.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEEILSQLDGGSVAVLPVIDSTNQYLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGSGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3068..4096)
                     /gene="murB"
                     /locus_tag="ACHKTW_004987"
     CDS             complement(3068..4096)
                     /gene="murB"
                     /locus_tag="ACHKTW_004987"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFO6465850.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQYATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTHLDPTTVTPQQVFNAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLAQFPTAPNYPQAGGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1125 a         1099 c          983 g         1183 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcgcgagc agcaccgccg taggttttag ccagtacggt agtgattgca
      121 gcggtcagcg ttgttttacc atggtcaacg tggccgatag taccgacgtt aacgtgcggt
      181 tttgtacgtt caaacttttc tttagacatc gattgtccct ctaagacacg gataaatcgg
      241 tgatatcacc acatcaacca ggcaacatgc ccgacttgtt gaatgcaata aacagaagga
      301 aaaaacaggg aggagaaaag aagtggtgct gataggcaga ttcgaactgc cgacctcacc
      361 cttaccaagg gtgcgctcta ccaactgagc tatatcagca catcttggag cgggcagcgg
      421 gaatcgaacc cgcatcatca gcttggaagg ctgaggtaat agccattata cgatgcccgc
      481 atcctggaac tcggctacct gattttcatt ctgcactgaa tatcgagaga agctctcttt
      541 attcgagccg gtaagcgaac ttatcgtctc gggctacgcc atcgcgtggc cgaaattggt
      601 ggtgggggaa ggattcgaac cttcgaagtc tgtgacggca gatttacagt ctgctccctt
      661 tggccgctcg ggaaccccac cggacttgat ggtgccgact accggaatcg aactggtgac
      721 ctactgatta caagtcagtt gctctaccta ctgagctaag tcggcatcaa gtagcgcgca
      781 ttctatggag acatgcgagt tcatgcaact aaaaaattgc ataatttgtt ttattggtca
      841 cattttatgc gacacgatga agaaacagcc gaacggaggg cagattagca cactttttca
      901 acatcattgt gctcaacaat gcgctcctgc taaaccataa ttctttttat cagatggaat
      961 atctgtcaca ttgcttttca acgatagctt cctggcagag attttttctt attattcctc
     1021 cccatctggt gttaccctcc tgcccattaa cccattcaac agaactgtga cgcgccatgg
     1081 caaatatcgc tttgccgata gagctatgac cgccagaaac atgcttatga gtataaaaga
     1141 gcaaacgtta atgacgcctt acctacagtt tgaccgcaac cagtgggcag ctctgcgtga
     1201 ttccgtacct atgacgttat cggaagatga gatcgcccgt ctcaaaggta ttaatgaaga
     1261 tctctcgtta gaagaagttg ccgagatcta tttacctttg tcacgtttgc tgaacttcta
     1321 tataagctcg aatctgcgcc gtcaggcagt tctggaacag tttcttggta ctaacgggca
     1381 acgcattcct tacattatca gtattgctgg cagtgtcgcg gtggggaaaa gtacaaccgc
     1441 ccgtgtattg caggcgctat taagccgttg gccggaacat cgtcgtgttg aactgatcac
     1501 tacagatggc ttccttcacc ctaatcaggt tctgaaagaa cgtggtctga tgaagaagaa
     1561 aggcttcccg gaatcgtatg atatgcatcg cctggtgaag tttgtttccg atctcaaatc
     1621 cggcgtgcca aacgttacag cccctgttta ctcgcatctt atttatgatg tgatcccgga
     1681 tggagataaa acggttgttc agcctgatat tttaattctt gaagggttaa atgtcttaca
     1741 gagcgggatg gattatccac acgatccaca tcatgtattt gtttctgatt ttgtcgattt
     1801 ttcgatatat gttgatgcac cggaagactt acttcagacg tggtatatca accgttttct
     1861 gaaattccgc gaaggggctt ttaccgaccc ggattcctat tttcataact acgcgaaatt
     1921 aactaaagaa gaagcgatta agactgccat gacattgtgg aaagagatca actggctgaa
     1981 cttaaagcaa aatattctac ctactcgtga gcgcgccagt ttaatcctga cgaaaagtgc
     2041 taatcatgcg gtagaagagg tcagactacg caaataattt tcaggggagc taatgctccc
     2101 ctttcttatt tttctgcact acgcagggat atttcaccgc ccatccaggg ttttattatt
     2161 ccatcctgct caagcaataa agcgccctgt ttgtctattc cgcgtgaaat gccaaatatt
     2221 tctttatcac caatgataag tttcactggg cgattaataa aattatccag cttttcccag
     2281 cgcgaaagat aaggtgccaa tccttcttgt tcgaagagtt ccagcgccgc gcgtaattca
     2341 cgtattagca tggccgccaa cgtattacga tcgagattga tccccgcttc ctgcagcgtg
     2401 atccacccct gattaacgac actctcttca acacgacgca ttgccatgtt gatcccggat
     2461 ccaatgacta tttgcgccgc atcgccggtt ttgccagtca gctccacaag aatgcctgcc
     2521 agcttgcgat cctgcagata gaggtcatta ggccatttaa cacgaacttt atctgcaccc
     2581 agcttgcgta atacttccgc catcacgata ccgataacca gacttaaacc aatagccgcc
     2641 gccgggcctt gttccaaacg ccagaacatc gacaaatata agtttgcgcc aaaaggcgaa
     2701 aaccatttcc gaccccggcg accacggcca gcctgctggt attctgcaac gcaggcatcg
     2761 cccgatttaa gctctccgat acgatcaaga aggtactgat tcgtggagtc aatcactggc
     2821 agcacggcta cactaccgcc atccagctga ctcaatatct cttcagcatt aagtaactgg
     2881 ataggttcag gcaggctgta tcctttaccc ggaacggtaa agacatcaac gccccagtca
     2941 cgcagtgtct gaatgtgttt attaatagcc gcccggctca ttcccagcgt ttcacccaac
     3001 tgctcgccag agtgaaattc accgttcgct aacagggcaa tcaatttcag tggcacggtg
     3061 ttatccttca tgaaattgtc tccactgcgc tcacttcacc tgatgcacca ataaagcgga
     3121 cttcaggctc aagccagaca ttaaattttt cacccacttt ttgtcttaca tggtgtgcca
     3181 gttgcaccac atcttcgctt tttgcattgt cttcattaat gagaactaac gcctgttgac
     3241 ggtgcaccgc agccccaccc atttgcatcc cttttagctg gcactgatca ataagccaac
     3301 ctgctgccag ttttactgaa ccacccgcct gggggtaatt tggtgctgtt ggaaattgcg
     3361 ccagtaatgc tttagccgtt tcggcagata caacagggtt tttgaagaaa ctaccggcat
     3421 tgccattcac ttttggatca gggagtttgg tggtgcgcat atgacacacc gcattaaata
     3481 cttgctgtgg cgttactgtt gtaggatcca gatgagttaa gtcaccatac gttagtacag
     3541 gttgccactc ttttggcaga cgcagaccta cggctacaat ggcgaagcga tcctggtatt
     3601 catgtttaaa aatactgtcg cgatagccaa aacggcactc tttggcagtt aagcgcactt
     3661 gcttgcctgt cgccagttca acacaatcaa cataagcgca aactcgctgt aattctacgc
     3721 cataagcacc aatattctgg ataggtgatg agccgacaca accaggaatt aatgccagat
     3781 tttccagacc aggcatacct tcctgcaaag tgtattttac caggcgatgc cagttttctc
     3841 cggctcctac atgtaaatac cacgcatcag gttcatcatg aatttcgata cctttgatcc
     3901 ggttgatgat caccgtgccg cgatagtctt ccagaaaaag tacattactt ccttcaccca
     3961 gaataagaac gggttgtcct tctgcggttg catactgcca ggcattgagt aattgttgtt
     4021 cgtcttcggc acatacaatg tgctgagcat tatgatcaat gccaaatgtg ttccagggtt
     4081 ttaaggagtg gttcatagct gctttcctga tgcaaaaacg aggctagttt accgtatctg
     4141 tggggggatg gcttgtagat atgacgacag gaagagtttg tagaaacgca aaaaggccat
     4201 ccgtcaggat ggccttctgc ttaatttgat gcctggcagt ttatggcggg cgtcctgccc
     4261 gccaccctcc gggccgttgc ttcgcaacgt tcaaatccgc tcccggcgga tttgtcctac
     4321 tcaggagagc gttcaccgac aaacaacaga taaaacgaaa ggcccagtct ttcgactgag
     4381 cctttcgttt
//
LOCUS       DAZFQD010000231         4346 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.233, whole genome shotgun sequence.
ACCESSION   DAZFQD010000231 DAZFQD010000000
VERSION     DAZFQD010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4346
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.233"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            241..582
                     /gene="raiA"
                     /locus_tag="ACHKTW_004988"
     CDS             241..582
                     /gene="raiA"
                     /locus_tag="ACHKTW_004988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFO6465851.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    685..810
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            686..733
                     /gene="pheL"
                     /locus_tag="ACHKTW_004989"
     CDS             686..733
                     /gene="pheL"
                     /locus_tag="ACHKTW_004989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheA operon leader peptide PheL"
                     /protein_id="HFO6465852.1"
                     /translation="MKHTPFFFAFFFTFP"
     gene            832..1992
                     /gene="pheA"
                     /locus_tag="ACHKTW_004990"
     CDS             832..1992
                     /gene="pheA"
                     /locus_tag="ACHKTW_004990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HFO6465853.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQQQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(2035..3156)
                     /gene="tyrA"
                     /locus_tag="ACHKTW_004991"
     CDS             complement(2035..3156)
                     /gene="tyrA"
                     /locus_tag="ACHKTW_004991"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HFO6465854.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGEVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(3167..4237)
                     /gene="aroF"
                     /locus_tag="ACHKTW_004992"
     CDS             complement(3167..4237)
                     /gene="aroF"
                     /locus_tag="ACHKTW_004992"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HFO6465855.1"
                     /translation="MQKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRRTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHRFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
BASE COUNT         1165 a         1132 c          996 g         1053 t
ORIGIN      
        1 tgagctgccg tttttttatt ctgttagttg tgaaactgaa gcgatttagt cactatcgat
       61 ctcatcaaat atggctcgct ttgagatatt cctcaagtaa aaaaacacct cttcctgcga
      121 tttctcacaa aaaagattcg ttgacaaaaa gtgacaaaat tatgagattc ccatcacaca
      181 ttttgacatc aggagcggta tgctgaattc accaagacgg gaagacaaga ggtaaaattt
      241 atgacaatga acattaccag caaacaaatg gaaattactc cggccatccg ccaacatgtc
      301 gcagaccgtc tcgccaaact ggaaaaatgg caaacacatc tgattaatcc acatatcatt
      361 ctgtccaaag agccacaagg gtttgttgct gacgccacaa tcaatacacc taacggcgtt
      421 ctggttgcca gtggtaaaca tgaagatatg tacactgcaa ttaacgaatt gatcaacaag
      481 ctggaacggc agctcaataa actgcagcac aaaggcgaag cacgtcgtgc cgcaacatcg
      541 gtgaaagacg ccaacttcgt cgaagaagtt gaagaagagt agtcctttat attgagtgta
      601 tcgccaacgc gccttcgggc gcgttttttg ttgacagcgt gaaaacagta cgggtactgt
      661 actaaagtca cttaaggaca caaacatgaa acacacaccg tttttcttcg cattcttttt
      721 taccttcccc tgaatgggag gcgtttcgtc gtgtgaaaca gaatgcgaag acgaacaata
      781 aggcctccca aatcgggggg ccttttttat tgataacaaa aaggcaacac tatgacatcg
      841 gaaaacccgt tactggcgct gcgagagaaa atcagcgcgc tggatgaaaa attattagca
      901 ttactggcag agcggcgcga attggccgtc gaggtaggaa aagccaaact gctctcgcat
      961 cgcccggtac gagatattga tcgtgaacgc gatttactgg aaagattaat tacgctcggt
     1021 aaagcgcacc atctggacgc ccattacatt actcgcctgt tccagctcat cattgaagat
     1081 tccgtattaa ctcagcaggc tttgctccag caacatctca ataaaattaa tccgcactca
     1141 gcacgcatcg cttttctcgg ccccaaaggt tcttattccc atcttgcggc gcgccagtat
     1201 gctgcccgtc actttgagca attcattgaa agtggctgcg ccaaatttgc cgatattttt
     1261 aatcaggtgg aaaccggcca ggccgactat gccgtcgtac cgattgaaaa taccagctcc
     1321 ggtgccataa acgacgttta cgatctgctg caacatacca gcttgtcgat tgttggcgag
     1381 atgacgttaa ctatcgacca ttgtttgttg gtctccggca cgactgattt atccaccatt
     1441 aatacggtct acagccatcc gcagccattc cagcaatgca gcaaattcct taatcgttat
     1501 cctcactgga agattgaata taccgaaagt acgtctgcgg caatggaaaa ggttgcacag
     1561 gcaaaatcac cgcatgttgc tgcgttggga agcgaagctg gcggcacttt gtacggtttg
     1621 caggtactgg agcgtattga agcgaatcag caacaaaact tcacccgatt tgtggtgttg
     1681 gcacgtaaag ccattaacgt gtctgaccag gttccggcga aaacgacgtt gttaatggcg
     1741 accgggcaac aagccggtgc gctggttgaa gcgttgctgg tgctgcgcaa ccacaatctg
     1801 attatgaccc gtctggaatc acgcccgatt cacggtaatc catgggaaga gatgttttat
     1861 ctggatattc aggccaatct tgaatcagcg gaaatgcaaa aagcattgaa agagttaggg
     1921 gaaattaccc gttcaatgaa ggtattgggc tgttacccaa gtgagaacgt agtgcctgtt
     1981 gatccaacct gatgaaaagg tgccggatga tgtgaatcat ccggcactgg attattactg
     2041 gcggttgtca ttcgcctgac gcaataacac gcggctttca ctctgaaaac gctgtgcgta
     2101 atcgccgaac cagtgctcca ccttgcggaa actgtcaata aacgcctgct tatcgccctg
     2161 ctccagcaac tcaatcgcct cgccgaaacg cttatagtaa cgtttgatta acgccagatt
     2221 acgctctgac gacataataa tgtcggcata aagctgcgga tcctgagcaa acagtcgccc
     2281 gaccatcgcc agctcaaggc ggtaaatcgg cgaagagagc gccagaagtt gctcaagctg
     2341 aacattttct tctgccagat gcagcccata agcaaaagta gcaaagtggc gcagagcctg
     2401 aataaacgcc atattctgat cgtgctcgac agcgctaata cgatgcagcc gagcgcccca
     2461 gacctgaatt tgctccagaa accattggta tgcttccggc ttacgtccat cacaccagac
     2521 cacaacttgc tttgccaggc taccgctgtc cgggccgaac atcgggtgta accccagtac
     2581 cgggccatcg tgcgccgcca gcatggcctg taatggtcca tttttcactg atgccagatc
     2641 aaccagaata caatctttcg gtaaaggcgg taatttgccg ataacttgct cagtaacgtg
     2701 gattggcaca ctaacaatca ccattccggc atcggcaaca atatcagccg ctcgatccca
     2761 gtcatgttgc tccagaatcc gcacctgata acccgatagt gtcagcatct tctcgaacag
     2821 gcgtcccatc tgaccgccac cgccgacgat aaccaccgga cgcagtgacg gacaaagtgt
     2881 tttaaatcct ttgtcgtttt cactggagta agattcacgc atcacccgac gcaaaacatc
     2941 ctcaatcaga tctggcggta cacccagagc ttccgcctct gcacgacgcg aggccaacat
     3001 agatgcctcg cgctccggaa cataaatagg cagtccaaag cggcttttca cctcgcccac
     3061 ttcagcaacc agttccagac gcttcgctaa taaattcagc agcgctttat cgacttcatc
     3121 aatttgatcg cgtaatgcgg tcaattcagc aaccataata aacctcttaa gccacgcgag
     3181 ccgtcagctg cccgttcaga tcctgatgaa tttcacgcag caaggcatcg gtcatttccc
     3241 agctaatgca ggcatcggtt acggatacac cgtatttcat ttcactgcgc ggttgctcag
     3301 aagactgatt gccctcgtgg atattacttt cgatcatcag accaataatt gagcgattgc
     3361 catctttgat ttgagcaacc acggattctg ccaccgcagg ctgacggcga taatctttat
     3421 tggaattacc gtggctgcaa tctaccatca gagacgggcg cagtcccgcc tgttccatct
     3481 ctttttcaca ttgcgcaaca tccgcagggc tatagttcgg cgctttacca ccgcgcagga
     3541 tgacatgccc gtccggattc ccctgagttt gtagcaacgc aacctgccct gcctggttaa
     3601 tgccaacaaa acggtgcggc tgggcggcgg cgcgcatagc gttaattgct gttgccagac
     3661 tgccgtcggt gccgttttta aaaccaaccg gcatggaaag cccggaggcc atttcacggt
     3721 gagtttgcga ttccgttgta cgagcaccaa ttgctgacca gctaaacaga tcgcccaggt
     3781 attgcgggct attcggatct aacgcttccg tcgccagtgg cagtcccata ttcaccagct
     3841 caagcagcaa tttacgcgcg atctgcagcc cggcttctac atcaaaagag ccatccatat
     3901 ggggatcgtt aattaaccct ttccagccga cagtggtacg gggtttttca aaatagacgc
     3961 gcattaccag atagaggcta tcgctgacct ctgcggcaag ggctttaaat cgacgagcat
     4021 attccagagc agtttccgga tcatgaatgg aacaaggacc acatactacc agcagacgag
     4081 gatcgcgccc ggcgataata tctgaaatgg ttctacgcga gtcagcaatc tgggcttctt
     4141 gttgcaggct caatggaaaa gcggccttca gttgttccgg agtcattaaa acctgttcgt
     4201 cggtaatatg tacgttattc agcgcgtctt tttgcatgat ggcgatcctg tttatgctcg
     4261 tttgcgatag ttgatcctca gcgaggatga cgtaacgata acacataaag taaagttttc
     4321 aatccatatt tcgtacattt ttcttt
//
LOCUS       DAZFQD010000232         4323 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.234, whole genome shotgun sequence.
ACCESSION   DAZFQD010000232 DAZFQD010000000
VERSION     DAZFQD010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4323
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.234"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..339
                     /locus_tag="ACHKTW_004993"
     CDS             <1..339
                     /locus_tag="ACHKTW_004993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaJ viral recombinase family protein"
                     /protein_id="HFO6465856.1"
                     /translation="ACSPDGLCSDGNGLELKCPFTSRDFMKFRLGGFEAIKSAYMAQV
                     QYSMWVTRKDAWYFANYDPRMKREGLHYVVIERNEKYMANFDEMVPEFIEKMDEALAE
                     IGFVYGEQWQ"
     gene            336..494
                     /locus_tag="ACHKTW_004994"
     CDS             336..494
                     /locus_tag="ACHKTW_004994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001598316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1317 family protein"
                     /protein_id="HFO6465857.1"
                     /translation="MKHPHDNIRVGAITFVYSVTKRGWVFPGLSVIRNPLKAQRLAEE
                     INNKRESV"
     gene            491..1243
                     /locus_tag="ACHKTW_004995"
     CDS             491..1243
                     /locus_tag="ACHKTW_004995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015964734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6465858.1"
                     /translation="MIHFHGGPITPDTCALKAWKGRHAFISFANPAQIDLASEVTQSF
                     ALDNGAFTFWTKNKAIDWNEYYRFVERWGNHPRFSFAVIPDVIGGTSEENDALIAAWP
                     HGKFIGAPVWHMNEPDERFIRLCHEFPRVCIGSMGEYDAKRPRACRAKLRDLIRHVVD
                     QYGYPITKIHGLRMLNKDIFTHVPLSSADSTNVARNIGIDKSWVGSPYAPASKETRTQ
                     VLVERIESFNSASSLNYNAERDVFTPQLAFEV"
     gene            1251..1466
                     /locus_tag="ACHKTW_004996"
     CDS             1251..1466
                     /locus_tag="ACHKTW_004996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFO6465859.1"
                     /translation="MTIEHNNALRSIARQANSEIKKARQQFPDKNVDDICRSVLKKHR
                     ETVTLMGFTPTHLSLAIGMLNGVFKER"
     gene            1565..1786
                     /locus_tag="ACHKTW_004997"
     CDS             1565..1786
                     /locus_tag="ACHKTW_004997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HFO6465860.1"
                     /translation="MADLIDSASEIEELQRNTAIKMRRLNHQAISATHCCECGDPIDE
                     RRRLAVQGCRTCASCQEDLELISKQRGSK"
     gene            1783..2598
                     /locus_tag="ACHKTW_004998"
     CDS             1783..2598
                     /locus_tag="ACHKTW_004998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFO6465861.1"
                     /translation="MSEINYQELREKAEKATKGSYIVGHTSVNQHGNLTGVFVCQKWK
                     GEPGGVIAECHVNCLVETDAQAYANAEFIAEANPATVLALLDERERNLQYIKSRDQEN
                     EDIALTVGKLRVELEEVKQHAEELSETKAVRNQWRPDICPITGRAFFMWIEHPTLGNV
                     PTYGGPLDSYTIPTKDGDGEFSCERYDHDFGGWVESECLGLYLIDDREQCRVYELEER
                     VKELDAREISLPERSSMLHRTDFHDDYQTVMAYKVSEVIAAIRAAGIRIKGGE"
     gene            2598..2774
                     /locus_tag="ACHKTW_004999"
     CDS             2598..2774
                     /locus_tag="ACHKTW_004999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6465862.1"
                     /translation="MRVACIGLLPYPTRFWASALIAKPHVLMADNIIPAPKRRHTGIA
                     AARRAAKKRRRTKR"
     gene            2949..3140
                     /locus_tag="ACHKTW_005000"
     CDS             2949..3140
                     /locus_tag="ACHKTW_005000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001420769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6465863.1"
                     /translation="MKNRKAKMLISRVYRRCYPSQWLRVSNRRVVLYLYSGIAREGIK
                     DKRSAAQNRWKNHLRTKGA"
     gene            3142..3351
                     /locus_tag="ACHKTW_005001"
     CDS             3142..3351
                     /locus_tag="ACHKTW_005001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000951717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6465864.1"
                     /translation="MAISASYTMHLYCDCCQCTNGKYQTPDFGEYIGTSWAGCAKEAR
                     KDGWRISKDKTRAFAPGHKVLRINK"
     gene            3348..3797
                     /locus_tag="ACHKTW_005002"
     CDS             3348..3797
                     /locus_tag="ACHKTW_005002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6465865.1"
                     /translation="MTTITRENAEIKSFITGFLSDPAHDNQSSSSLLANVFRIALASL
                     EAEPVAWRYRYVKKGVTDSQGDSWVGDWKYVPTKEDCNDRPNYEIQALFTAPPVPLTS
                     EGLIKAVRFYEQVKRENPPVETGAWKDAVDWVLKEACQAVNIGIKGE"
     gene            4022..4210
                     /locus_tag="ACHKTW_005003"
     CDS             4022..4210
                     /locus_tag="ACHKTW_005003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6465866.1"
                     /translation="MSLTVRQLISKLRKMPPEAVVVWQGYEQSECEYNDFVGHVADIT
                     DENSSSFDPEVRVVALRG"
     gene            4214..>4323
                     /locus_tag="ACHKTW_005004"
     CDS             4214..>4323
                     /locus_tag="ACHKTW_005004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6465867.1"
                     /translation="MITITKGRLLTIKQWRETYGPGSNVVLPAEEAEELAR"
BASE COUNT         1256 a          930 c         1141 g          996 t
ORIGIN      
        1 gcctgctctc ccgatggttt atgcagtgac ggcaatggcc ttgagctgaa atgcccgttt
       61 acctccaggg atttcatgaa gttccggctc ggtggtttcg aggccataaa gtcggcttac
      121 atggcccagg tgcagtacag catgtgggtg acgcgaaaag atgcctggta ctttgccaac
      181 tatgacccgc gtatgaagcg tgaaggcctg cattatgtcg tgattgagcg gaatgaaaag
      241 tacatggcga attttgacga gatggtgccg gagttcatcg aaaaaatgga cgaggcactg
      301 gctgaaattg gttttgtata tggggagcaa tggcaatgaa gcatcctcac gataatatcc
      361 gggtaggcgc gatcactttc gtctactccg ttacaaagcg aggctgggta tttcccggcc
      421 tttctgttat ccgaaatccg ctgaaagcac agcggctggc tgaggagata aataataaac
      481 gggagagtgt atgattcatt ttcacggtgg tccaataact cccgatacct gtgcgttgaa
      541 agcctggaaa ggcagacacg cattcatcag ttttgctaat ccagctcaga ttgatctggc
      601 ttccgaagtc acccaatcat ttgctcttga taatggtgca ttcacattct ggacaaaaaa
      661 caaggccata gactggaatg aatattacag atttgttgaa cgctggggta atcaccctcg
      721 tttctcattc gcggttatcc cggatgttat cggcggaacc agtgaagaga atgacgccct
      781 gattgcggca tggcctcacg gtaaatttat tggtgctccg gtgtggcaca tgaacgaacc
      841 agatgagcga tttattcgtc tgtgccatga gtttccccgc gtctgcatcg gctcgatggg
      901 ggaatacgat gcaaaacgac cgagagcgtg tcgggctaaa ctacgcgatc ttatacgtca
      961 tgttgtagat cagtacggct acccaatcac caagattcat gggttacgga tgttaaataa
     1021 agacattttt actcatgtac cgctttcgtc tgcagacagc acgaacgtcg caagaaatat
     1081 aggcatcgac aaatcctggg taggttcgcc atatgctccc gcaagtaaag aaacccgtac
     1141 gcaagttctt gtggaacgca ttgaatcatt caacagtgcc agttcactta attacaacgc
     1201 tgaacgggac gtctttaccc ctcaactggc attcgaagtg tgaggccaat atgacaatag
     1261 aacataataa cgcccttcgc agcattgccc gtcaggctaa ttctgaaatc aaaaaagcca
     1321 gacagcagtt tccggataaa aacgtcgatg acatttgccg tagcgtactg aagaagcacc
     1381 gcgaaacggt aacgctgatg ggattcacac cgactcactt aagtctggca atcggtatgt
     1441 taaacggcgt ctttaaggaa cggtgaacat gaaaagcaaa atcatcaggg agctacaggc
     1501 tcctttttta ttattcgcat tcaccctcaa gcgtattaac caacaattca gggattaatg
     1561 gaagatggca gacctcattg attcagcatc agaaattgaa gaattacagc gcaacacagc
     1621 aataaaaatg cgccgcctga accaccaggc tatatctgcc actcattgtt gtgagtgtgg
     1681 cgatcccata gatgagcgaa gacgcctggc cgttcagggt tgtcggactt gtgcaagttg
     1741 ccaggaggat ctggagctta tcagtaaaca gagaggttcg aagtgagcga aattaactat
     1801 caggaactgc gtgaaaaggc agagaaagca actaaaggaa gctacatcgt agggcataca
     1861 tctgttaacc aacacggcaa tttaacagga gtttttgttt gtcaaaaatg gaaaggagaa
     1921 cccggtggcg taattgcaga atgtcacgtt aactgcctgg ttgaaacaga tgctcaggct
     1981 tatgcaaacg ctgaattcat agcagaggct aacccggcta ccgtgctggc actgctggat
     2041 gaacgggaaa gaaacctgca atacatcaaa agccgcgatc aggagaacga ggatattgcg
     2101 ctaacggtag ggaagctgag agttgagctt gaggaagtaa aacaacacgc tgaagaatta
     2161 tccgaaacca aggctgttcg taaccaatgg cggccagata tttgcccaat aaccggacgt
     2221 gcatttttca tgtggattga gcatccaaca ttgggaaatg tgccgacata tggtggccca
     2281 ttagatagtt acaccattcc aacaaaggac ggtgacggtg agttttcatg tgagcgttac
     2341 gatcatgatt ttggcggttg ggtagaaagc gaatgtcttg ggttatatct gattgatgat
     2401 agagaacaat gcagggtcta cgaactggag gaacgcgtta aggaactgga tgctcgggaa
     2461 atatcgctcc cggaacgtag cagcatgctt catcgaacag attttcacga tgattaccaa
     2521 acggtaatgg catacaaagt ttctgaagtc atcgctgcaa tccgcgccgc aggcattcgc
     2581 atcaaaggag gtgagtaatg cgtgtggcat gtatcggctt gttaccgtac ccgactcgtt
     2641 tttgggcttc tgcgctaatt gcaaagccac atgtcctgat ggctgacaac atcatcccgg
     2701 caccaaagcg ccgccatacc ggtattgcag cggcacgacg agcagcaaag aaacgcagga
     2761 gaacaaaacg atgaaaaacc gtaaggcaaa aattctgtta gctcgcagaa acggtgttgg
     2821 ggtctggcga tggttgagga ttagtaacag acgagtgagg ttgacggggt gttgcggggt
     2881 gatgggcaca gttgttgcaa aaagcctagc gcgtcacaga acaggcggaa gaaagggtac
     2941 gcagtaagat gaaaaaccgt aaagcaaaga tgcttatctc ccgtgtatac agacgttgct
     3001 atcccagcca gtggttgaga gttagcaatc gccgtgtggt gttgtactta tattctggaa
     3061 ttgccagaga gggaatcaaa gataagcgca gcgcggcgca aaaccgctgg aaaaaccact
     3121 tacgcactaa aggagcctga tatggctatt tccgcaagtt acaccatgca tctctactgt
     3181 gattgttgcc agtgtacaaa tggtaaatat caaacgccag acttcggtga gtatatcggt
     3241 acgtcatggg ctggctgtgc aaaagaggcg cgcaaggatg gctggcgaat aagcaaagac
     3301 aaaacacgtg cttttgcgcc cgggcataaa gttttgagga ttaataaatg accactatta
     3361 ccagagaaaa cgcggaaatt aaatcattca tcactggttt cctgagcgac ccggcgcacg
     3421 ataaccaatc ttcaagcagc ctgcttgcca atgtgtttcg tatcgcactg gcatcgttgg
     3481 aagcagagcc agtcgcgtgg cgatatcgct acgtgaaaaa aggtgttacg gactctcagg
     3541 gggattcgtg ggttggtgac tggaaatatg taccgacaaa agaggattgc aacgacagac
     3601 cgaactatga aattcaggcg ttattcactg ccccgcctgt gccactgaca tcagaaggat
     3661 tgattaaagc agtgcgtttc tatgaacagg ttaagcgtga aaatccgcca gtcgaaaccg
     3721 gagcatggaa agacgctgtt gactgggtac tcaaagaggc ttgccaggct gtaaacattg
     3781 gcatcaaagg agagtgatat ggctaactca ttacttgaaa cctgcaacaa ctagcagatt
     3841 cagagggcgg agattttatc tcgcaatcca gatatggcaa tgacaattga taacctggat
     3901 acgctaattg aacggaccgt gcgttctgca attgatatag cacatcgagt agattgggat
     3961 ttcagagaag cggagcgact cgctaaagag caggcgaaag cagcgggtaa aggagagtga
     4021 tatgtcactg acggtgaggc aattaatcag caagctcaga aaaatgcctc ctgaggctgt
     4081 cgtggtctgg cagggctatg aacaatcaga gtgtgagtat aacgactttg taggtcatgt
     4141 tgctgatatt acagatgaaa attcatcatc ctttgaccca gaagtgcgcg ttgtggcgct
     4201 aagaggataa cccatgatca ctattaccaa agggcgactg ctgacaatca agcagtggcg
     4261 cgaaacatac ggaccgggta gcaacgttgt actgccagca gaagaagcgg aagaactggc
     4321 acg
//
LOCUS       DAZFQD010000233         4317 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.235, whole genome shotgun sequence.
ACCESSION   DAZFQD010000233 DAZFQD010000000
VERSION     DAZFQD010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4317
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.235"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(40..498)
                     /locus_tag="ACHKTW_005005"
     CDS             complement(40..498)
                     /locus_tag="ACHKTW_005005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6465868.1"
                     /translation="MKIYWTRKSIPELSALPPSLRKKNFTDAYNAASSHIEYWIGAGV
                     SFISMMILFRVYDFLLPAQDTFPGDIIRSLCVVCPSILIWFQFSVYVMRKYYRHILER
                     GKETETISERLIREADTREYELWRPARRFFSIVFLIVLLVCIHSLITTIK"
     gene            956..1465
                     /locus_tag="ACHKTW_005006"
     CDS             956..1465
                     /locus_tag="ACHKTW_005006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6465869.1"
                     /translation="MQITEALISEPGEIRRFVQQAVDHWPNLLAFHFTLYSAEGIYGQ
                     QIQTFCSSFHRRVHERITEHNHTVSPSAPVVLRWLREQHEGAQIRCLLLLSQTSICHP
                     RVGVMADEECAQLVDLLQQTWSVISAGGQCRVERCFRVARPGSSGQYVALKTAVQSFM
                     SQVIATIIR"
     gene            1554..2192
                     /locus_tag="ACHKTW_005007"
     CDS             1554..2192
                     /locus_tag="ACHKTW_005007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001518292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO6465870.1"
                     /translation="MRTRIDYLADKYSFTELNERPHLRRQWQDVLEECRQTEAGPEER
                     LRIALLNVDYVTSFELPFRLLLIRTPQLIAALREEWDIGQKNVLFNDKRFGCVYSLKS
                     SLSGVPDTYRYHLSHRIRRVVANENTSLPYQQIAREVKAPRERLKYALETGLQVTALD
                     GLFWFGSQRIAADIQRLRKSGMRITTSAISVYDSITVTRRLCPVYRIVANNK"
     gene            2529..4148
                     /locus_tag="ACHKTW_005008"
     CDS             2529..4148
                     /locus_tag="ACHKTW_005008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003845607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HFO6465871.1"
                     /translation="MARIPTKKAKAKKGFEETLWDAANQLRGSVESSEYKHVVLSLVF
                     LKFISDKFETRRKKMIADGQADFLEMEVFYQQDNIFYLPEEARWSFIKQNAKQDDIAV
                     RIDTALSTIEKRNPTLKGALPDNYFSRQNLETKKLASLIDTIDNIETLAHETDVETLS
                     KEDLVGRVYEYFLGKFAATEGKGGGEFYTPKCVVTLLTEMLEPFQGKIYDPCCGSAGM
                     FVQSVKFVESHQGKSRDIALYGQELTATTYKLAKMNLAIRGLSANLGERPANTFFSDQ
                     HPDLKADYILANPPFNLKDWRNEAELTKDPRFAGYRMPPTGNANYGWILHMLSKLSAN
                     GTAGFVLANGSMSSNTSGEGEIRAQMIENDLIDCMIALPGQLFYTTQIPVCLWFMTKS
                     KAADPAKGYRDRQGETLFIDARNLGTMISRTTKELTAEDIATIADTYHAWRSTPEELA
                     ARIARGDSKLEKYEDQAGFCKVATLQDIKDNDYVLTPGRYVGAAEQEEDGVAFETKMR
                     ELSKTLFEQMKQAEELDRAIRQNLEALGYGE"
     gene            4138..>4317
                     /locus_tag="ACHKTW_005009"
     CDS             4138..>4317
                     /locus_tag="ACHKTW_005009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001518290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFO6465872.1"
                     /translation="MGSKWEKIKLKEVVDIITTKVDVSQISLCDYISTENMLTNFGGI
                     SIANSKPSTGKITKFH"
BASE COUNT         1215 a          918 c         1047 g         1137 t
ORIGIN      
        1 atgaacgttt attttcctgt atcagaacag ccataacttt tattttatag ttgttatcag
       61 tgaatgaatg caaactaata gaacaatgag aaaaacaatg gagaaaaagc gacgggccgg
      121 acgccatagt tcatattctc gtgtatcagc ctcacgtatc aacctttctg aaatggtttc
      181 ggtttcctta cctcgttcca gaatatgtct gtaatatttc ctcatgacat ataccgaaaa
      241 ctgaaaccag attaatatgg atggacacac aacacataaa cttctgatga tatcccccgg
      301 gaaagtatct tgggcgggta aaagaaagtc atatactctg aataaaatca tcatcgagat
      361 aaatgagacg ccagcaccta tccagtattc gatatgggaa gatgcagcat tatatgcgtc
      421 agtaaaattt ttctttctta atgaaggtgg aagtgctgaa agttcaggaa tgctttttct
      481 tgtccagtat attttcatat ggcaactcat ataaaagtgt tcatacttta ctatgatgat
      541 gacgatttat aaaaggctta taatgactac ttctcctttc attctcgaat ttcacgataa
      601 tgatagagat aatcattatc aattgattgt gtcagtaatt ttgacttaca tcacgctttc
      661 atggtttaat caggtgattg atttaatgaa aagagtgtgt ttgtggatgt attgtgtata
      721 tgtcttaccg ggcatctgtt cttacattaa ttaatatatt gctatttaag tattggtggt
      781 attgtcagta acattctttt ggttaattta gttattattc ctcactgatt aattgtcctt
      841 ccgttcttat tgtttgtaac ttgtgtttgt atcgtacctt tcaggtgttg atgcgcgctt
      901 tactgtgtct gcaccggctt gacaaaattc aacagggttt gaaaaggaac atttcgtgca
      961 aataaccgaa gccttaattt cagagccggg ggaaatccgg cgttttgttc agcaggcggt
     1021 ggaccactgg ccaaatctgt tggcatttca cttcacgctc tattcggcag aaggaatcta
     1081 tggtcaacag attcagacat tttgtagttc ctttcaccga cgggtacacg aacgtattac
     1141 ggagcataat cacacggtca gcccatcagc gccggtggta ttacgctggt tgcgggaaca
     1201 acatgaagga gcacagatac gatgtctgtt gctgctcagc cagacgagta tttgccatcc
     1261 gcgagtcggc gtcatggctg atgaagaatg tgcgcaactg gtggatttac tgcaacagac
     1321 ctggtcggtg ataagtgctg gcggacaatg ccgggtggaa agatgttttc gggttgcccg
     1381 gcctggttca tcagggcaat acgtggcgtt aaaaacggct gttcagtcct ttatgtcgca
     1441 ggttattgca acaatcatcc ggtgattgtc gggaggaata tcaatgcttc cgttaatttt
     1501 tactggctga taaagttttg tctgctgaat ctttatacgg aattcctttc agtatgcgta
     1561 cacgaattga ctatcttgca gataaataca gttttactga actgaatgaa cgtccacacc
     1621 ttcgccggca gtggcaggat gttctggagg agtgtcggca gacagaggcc gggccagaag
     1681 aacggctgcg gattgccctg ttgaatgtgg attacgtcac cagttttgaa cttccatttc
     1741 ggttgctgct tatccgtaca ccacaactga ttgctgcgtt gcgtgaagaa tgggacatcg
     1801 gccagaaaaa tgtgctgttt aacgataaac ggtttggttg cgtgtacagc ctgaagtcca
     1861 gtctttctgg tgtaccggat acataccggt atcatctgtc tcaccgtatt cgtcgtgtgg
     1921 ttgcgaatga aaacacatca ttgccatatc agcagattgc ccgggaagtg aaagcccccc
     1981 gggaacgact gaagtatgcg ctggagacgg gattgcaggt gaccgcactg gacggcttgt
     2041 tctggttcgg tagccagcgc attgctgcag atatacagag actgagaaaa tctggtatga
     2101 ggattaccac ttctgccata agcgtgtatg actctattac cgtaacccgg agattgtgcc
     2161 cggtataccg gattgtcgcg aataataaat aaacggtaat gctgagtttc tccattacca
     2221 ttgcaaatga ctcaccagag cagactgaac agcgcagaag tgggattgtg gatacgtgaa
     2281 gtgagagtaa ggggaaaatc cacaataatc atctatcgaa cagggaggcg aactttacac
     2341 gatggttttc cgggagtgct tacccggggt tcctcacctc tggctaatct ctggattgag
     2401 tcgcgatact ccaacaaaag caacaagcta acgcagcaag atgttaacgc tcatcgagag
     2461 taaaatgcac acttttatgg cttactcgtt acaataacag ccagtttgtt cagaaaaccg
     2521 gattcagtat ggccagaata ccaaccaaaa aagctaaagc aaaaaaaggg tttgaagaaa
     2581 cattatggga tgccgcaaat cagcttcgcg gcagcgttga gtcctccgaa tacaagcacg
     2641 tggtgttgag cctcgtgttc ctgaaattca tcagcgataa gtttgaaaca cgccgcaaaa
     2701 aaatgattgc cgatgggcag gcagatttcc ttgagatgga agtgttctac cagcaggaca
     2761 acattttcta cctgccggaa gaggcgcgtt ggtcatttat caaacaaaat gcaaaacagg
     2821 acgatattgc ggttcgtatt gacaccgccc tctcgaccat tgagaaacgt aacccaaccc
     2881 tgaaaggtgc gctgccagac aactacttca gccgtcagaa tctggaaacc aaaaaactgg
     2941 catcactgat tgataccatc gacaacatcg aaacgctggc acacgagact gacgttgaaa
     3001 cgttatcgaa agaagacctg gtcggacgcg tttatgaata cttcctcggt aagtttgccg
     3061 ccactgaagg caaaggcggt ggtgagttct acacgccaaa atgtgtggtc acgctgttaa
     3121 ctgaaatgct cgaacccttc cagggcaaaa tttatgaccc gtgctgcggc tcggcaggaa
     3181 tgttcgtgca gtcggtgaag tttgtcgaga gccatcaggg taaaagccgt gatatcgcgc
     3241 tgtatggtca ggagctgaca gccacgacgt ataaactggc aaaaatgaac ctcgctattc
     3301 gcggtctttc agctaacctc ggcgaacgcc cggcaaacac tttctttagc gaccagcacc
     3361 cggacctgaa agctgactat attctggcga acccgccgtt caacctgaaa gactggcgta
     3421 acgaagcaga attaaccaaa gacccacgtt ttgccggtta tcgtatgccg ccaaccggta
     3481 acgccaacta cggctggatt ttgcatatgc tctccaagct gtcggctaac ggcacagcgg
     3541 gttttgtgct ggcaaacggt tcgatgagtt ctaacaccag cggtgaaggc gagatccgtg
     3601 cacagatgat cgaaaatgat ctgatcgact gcatgattgc tctgccaggt cagttgtttt
     3661 acaccacgca gatcccggtg tgtttatggt ttatgaccaa atcgaaggct gccgatccgg
     3721 ccaaaggtta tcgtgatcgt cagggcgaga cgctgtttat tgatgcgcgt aacctcggca
     3781 ccatgattag ccgcacaact aaagagttaa cagcggaaga tattgccaca atcgccgata
     3841 cttaccatgc ctggcgtagc acgccagaag aactggctgc acggattgcg cgtggtgaca
     3901 gcaagctgga aaaatatgaa gaccaggcag gcttctgcaa agttgcgacc ctgcaagata
     3961 ttaaagataa cgactacgtt ctgacaccgg gccgctatgt gggtgcagcc gagcaggaag
     4021 aagacggcgt ggcatttgag accaaaatgc gtgaattgtc gaagacgttg tttgagcaga
     4081 tgaagcaggc ggaagaactg gatcgtgcga ttcgccagaa tctggaggcg ctgggttatg
     4141 gggagtaaat gggagaaaat aaaacttaaa gaagttgtag atattatcac tactaaagtt
     4201 gatgtatcgc aaattagtct ttgcgattac atatcaactg aaaatatgct taccaatttt
     4261 ggaggtatat caatagcaaa tagtaaacct agcacaggga aaataacaaa atttcat
//
LOCUS       DAZFQD010000234         4285 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.236, whole genome shotgun sequence.
ACCESSION   DAZFQD010000234 DAZFQD010000000
VERSION     DAZFQD010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4285
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.236"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..546
                     /locus_tag="ACHKTW_005010"
     CDS             <1..546
                     /locus_tag="ACHKTW_005010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4329 domain-containing protein"
                     /protein_id="HFO6465873.1"
                     /translation="LYGYQLNPISDIDPLGLSMWEDAKSGACTNGLCGTLSAMIGPDK
                     FDSIDSTAYDALNKINSQSICEDKEFAGLICKDNSGRYFSTAPNRGERKGSYPFNSPC
                     PNGTEKVSAYHTHGADSHGEYWDEIFSGKDEKIVKSKDNNIKSFYLGTPSGNFKAIDN
                     HGKEITNRKGLPNVCRVHGNM"
     gene            518..901
                     /gene="yhhH"
                     /locus_tag="ACHKTW_005011"
     CDS             518..901
                     /gene="yhhH"
                     /locus_tag="ACHKTW_005011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417940.2"
                     /note="This BlastRule distinguishes YhhH from its paralog
                     YbbC. Both are named for founding members from Escherichia
                     coli K-12, and belong Pfam family PF15631; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhhH"
                     /protein_id="HFO6465874.1"
                     /translation="MSAEFMVICKKILFRNCVIVSLFVFTYNTWAQCNNNIKIMRKYE
                     SEGKYTVRNLVKNKAIALELAEIYVKNRYGQDAAEEEKPYEITELTTSWVVEGTIHSD
                     QIAGGVFIIEIGKNDGRILNFGHGK"
     gene            1006..1848
                     /gene="yibA"
                     /locus_tag="ACHKTW_005012"
     CDS             1006..1848
                     /gene="yibA"
                     /locus_tag="ACHKTW_005012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HFO6465875.1"
                     /translation="MSNTYQKRKASKEYGLYNQCKKLNDDELFRLLDDHNSLKRISSA
                     RVLQLRGGQDAVRLAIEFCSDKNYIRRDIGAFILGQIKICKKCEDNVFNILNNMALND
                     KSACVRATAIESTAQRCKKNQIYSPKIVEQSQITAFDKSTNVRRATAFAISVINDKAT
                     IPLLINLLKDPNGDVRNWAAFAININKYDNSDIRDCFVEMLQDKNEEVRIEAIIGLSY
                     RKDKRVLSVLCDELKKNTVYDDIIEAAGELGDKTLLPVLDTMLYKFDDNEIITSAIDK
                     LKRS"
     gene            1876..2834
                     /locus_tag="ACHKTW_005013"
                     /pseudo
     CDS             1876..2834
                     /locus_tag="ACHKTW_005013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312497.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
     gene            2846..3307
                     /gene="yibG"
                     /locus_tag="ACHKTW_005014"
     CDS             2846..3307
                     /gene="yibG"
                     /locus_tag="ACHKTW_005014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HFO6465876.1"
                     /translation="MKACLLLFFYFSLIGQLHGADVKIKENESVMGSTAMTYDLSEEK
                     LMKLKYKSQHGDSEASFRLYQYYCFTKNNIDKQLRFLERSASQGNVTAQFNYGVFLSD
                     TNPTLSEYYNLNRAIYWMEFAVNNGNIDTKSKLQELKKLKRMDRRKNKENP"
     gene            3942..>4285
                     /locus_tag="ACHKTW_005015"
     CDS             3942..>4285
                     /locus_tag="ACHKTW_005015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001626253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Imm57 family immunity protein"
                     /protein_id="HFO6465877.1"
                     /translation="MNNCIFFSISASAVTARQFQRELDLADKAILWALISASNKEGRK
                     ACSLSYFACKAAEAELGLAYMAANDNKEFLTSLSNIMRYKIDAGLSESYTCYLLSKGK
                     IIRPYLKNLNPL"
BASE COUNT         1434 a          713 c          947 g         1191 t
ORIGIN      
        1 ctgtatggat atcaattgaa tccgatatca gacatcgacc ccctgggttt atctatgtgg
       61 gaggatgcaa aatcgggggc atgtactaat ggtctttgcg gcacactatc cgctatgata
      121 ggtccagata aatttgattc tatagatagc accgcatatg acgccttaaa taaaataaat
      181 agccaatcta tttgcgaaga taaagagttc gctggtttaa tatgtaagga taatagtggc
      241 agatatttct caacagcacc taaccgagga gaaagaaaag gatcatatcc attcaatagc
      301 ccttgcccta atggtactga gaaagtatca gcttatcata ctcatggtgc agatagtcat
      361 ggagaatatt gggacgaaat attttcaggt aaagatgaga aaatagttaa aagtaaagat
      421 aacaatatca agtcatttta tttaggtacg cccagtggta attttaaagc aatagataac
      481 cacgggaagg aaataacaaa cagaaaagga ttacctaatg tctgcagagt tcatggtaat
      541 atgtaaaaaa atattgttta ggaactgtgt cattgtatct ttgtttgttt ttacatacaa
      601 cacatgggcg cagtgtaata ataatattaa aataatgcgc aagtatgaaa gtgaaggtaa
      661 atataccgtt agaaatttgg ttaaaaataa agctatagca ttggaattag ctgagatata
      721 tgttaagaat cgttatggac aggatgccgc agaagaagaa aaaccatacg aaatcactga
      781 gttaacaaca agttgggttg ttgaaggtac cattcactca gaccaaattg ctggtggggt
      841 ttttattata gaaataggca aaaatgatgg gagaattctg aattttggcc acggaaaata
      901 agtatgtaag gataatcaaa catcgtgtgc gctgatggca gagtaggtgg aggactccag
      961 acagtcaaac gatagaaaaa gatagccttt atggaggttc ctgcaatgtc aaatacatac
     1021 cagaaaagaa aggcaagtaa agagtatggt ttatataatc aatgtaagaa actaaatgat
     1081 gatgaattat ttcgcttact tgatgatcac aattccttga aaaggatttc atctgccaga
     1141 gtattacagt taagaggtgg gcaagacgct gttagattgg caattgagtt ctgctctgat
     1201 aaaaattata tccgtagaga tatcggagca tttatactcg ggcaaataaa aatttgcaaa
     1261 aaatgcgaag ataatgtttt taatattttg aacaatatgg cattgaatga taagagcgct
     1321 tgcgttcgag ctacggcaat cgagtcaacg gctcagcgat gcaagaaaaa ccaaatttat
     1381 tcacctaaaa tagtagaaca atctcaaatt actgcttttg ataaatcgac taatgtcaga
     1441 cgtgctacag catttgctat ttctgttatc aatgataaag caacaattcc actattgatt
     1501 aatctgttaa aagatccaaa tggagatgtc agaaactggg ccgcatttgc aataaatatc
     1561 aataaatatg ataatagtga tattagggat tgttttgtgg agatgcttca ggataaaaat
     1621 gaggaagtcc gtattgaagc aataatcgga ctttcctaca gaaaagataa aagggtttta
     1681 tctgttttat gcgatgagtt aaaaaaaaat actgtttatg atgatatcat tgaagctgcg
     1741 ggtgaattag gtgataaaac gctacttcct gttttagata ctatgttgta caagtttgat
     1801 gacaatgaaa ttataacttc cgctattgat aagctgaagc gttcatagcg tgagttgcct
     1861 atgcacagtg ggggattccc gccggcacgg tgcagatgct cgaccggctg gaaagtgaaa
     1921 tcctggctga ccgggtgagt gaggaaagcc gccgctggct ggcatcgtgc ggcctgaccg
     1981 tggagcagat gaaaaaccag atggacccgg tgtacacgcc ggcgcgaaaa atccacctgt
     2041 accactgcga ccatcgcggc ctgccgctgg cgctcatcag cacggaaggg gcaacagcgt
     2101 ggtgcgcaga atatgatgaa tggggcaacc tgctgagtga tgagaacccg catcatctgc
     2161 agcagctcat tcgcctgccg ggtcagcagt atgatgagga gtccggcctg tattacaacc
     2221 gccaccgcta ttatgacccg ctgcaggggc ggtatatcac tcaggatccg attgggctga
     2281 aggggggatg gaatttttat cagtatccgt tgaatccggt cataaatgta gatccgcaag
     2341 gtttggttga tataaattta taccccgaaa gtgatcttat ccattctgta gctgatgaga
     2401 ttaatatccc aggcgttttc acaatcgggg ggcatggtac ccccacatct attgaatccg
     2461 caacgcgcag tatcatgaca gctaaagatc tagcatatct aattaaattt gatgggaatt
     2521 ataaagatgg gatgacagtt tggttatttt cttgtaatac aggtaaagga caaaattcat
     2581 ttgctagcca attagctaaa gagttacata caaatgtaat aggacctgac acgctatgga
     2641 cgtggtgggg gcgaggaact aatggtaagt taaaaatgga tacagtgcta acagcaccaa
     2701 cgaaccttaa ttcaaataag gatctaatgg ctataacaac aaaagacctt ggtaattgga
     2761 taacatatgg gccatctggg caccccattt ctaatatgca aggtacgcca gaaaaaccca
     2821 gtgatataag ataggttgta gatgtatgaa agcatgcttg ttactatttt tttatttttc
     2881 tcttattggt caattgcatg gtgctgatgt gaaaataaaa gaaaacgaaa gtgtgatggg
     2941 atctacagca atgacctatg atttaagtga agaaaagctg atgaaactga agtataaatc
     3001 acaacatggt gattcagagg catcatttcg actatatcaa tattactgct ttactaaaaa
     3061 taatattgat aaacaattgc gattcttgga aagatcagca tctcagggga atgttacagc
     3121 gcagtttaat tatggggtct ttttatcaga tacaaatcca acattatcag aatattataa
     3181 tttgaataga gctatttatt ggatggaatt cgctgtaaat aacggtaata ttgatacaaa
     3241 gagcaaactt caagagctca aaaagctaaa gcgaatggat agaaggaaga ataaggagaa
     3301 tccgtaaaag caatagttca tcagactgcc gcagcagtag tatgatgatg aatcagggct
     3361 gtactataac tattatcggt aacattaacc gcagcagggg cggtataaca ctcaggctcc
     3421 gattgggctg gaaggtaggt ggaattttta tcagtatccg ttgaacccga tttctggaat
     3481 cgatcccctt gggattagcc acctgtttat acagtataac tttaggtatg ctttcgtgtg
     3541 tttcagatat accatgtgat gataattttt atgatgtgct tagtattcca gtcgcttctg
     3601 gtaataatat gcaatgtaaa aataatcccc gttgtacaca tttataaata gatggccaat
     3661 acctcaaggc ctatggagtt ggaatgttaa tgacccggga gcaagcaata gaaaacctga
     3721 cggcatacga ttagtaccgt cagttaatac aggaacttat aatcgcaatg gattttcaat
     3781 acatagttgt ttaaacgctt ttggtccgtc tttaggacct cgattctgtt ccgagggttg
     3841 tataactggt ttatctaatg acatgcaaaa attaaatgag ttaatatttt cagagccaga
     3901 tagcacatta acagttacgg actaaaaaat atggcaaggt tattaacaat tgtatttttt
     3961 tctctatttc tgcgagtgca gttactgcaa gacaatttca gagagagtta gatttggcag
     4021 acaaagcaat attatgggca ctaatatcag ccagtaataa agagggacga aaagcgtgtt
     4081 cactaagcta ttttgcctgt aaggccgcag aggctgaact cggactggca tatatggctg
     4141 cgaatgataa caaggaattt ctcacatcct tatctaatat aatgaggtac aaaatagatg
     4201 ctggactttc cgaatcctat acatgctatt tattaagtaa gggaaagatc ataagaccat
     4261 atctgaaaaa tctaaatcca ctcca
//
LOCUS       DAZFQD010000235         4217 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.237, whole genome shotgun sequence.
ACCESSION   DAZFQD010000235 DAZFQD010000000
VERSION     DAZFQD010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4217
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.237"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..345
                     /locus_tag="ACHKTW_005016"
     CDS             <1..345
                     /locus_tag="ACHKTW_005016"
                     /inference="COORDINATES: protein motif:HMM:NF033894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EexN family lipoprotein"
                     /protein_id="HFO6465878.1"
                     /translation="KKNTRVRHSSPFSHKKGNVMKSLKYILIGTIYLTAAVCNAEPQV
                     KTAEWYMQHHDEVLSKYKECKAAQDKSYGGTGYSDDCKNVLKASLRIKDPDFIKSLNK
                     IRLESINEILNK"
     gene            355..1404
                     /locus_tag="ACHKTW_005017"
     CDS             355..1404
                     /locus_tag="ACHKTW_005017"
                     /inference="COORDINATES: protein motif:HMM:NF016491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030255 - protein secretion by the type IV
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="HFO6465879.1"
                     /translation="MAGFFEKMNEKIDSLLTATSEGMSGKYSDSISLIAASALVLYGV
                     ILAFYIMGGKKKIEDVGSTFFHAMIIYVFVTNAGGYLNILSDGIKGLNDGLATVGGAG
                     SSAWSLLDKQWEVTQRLAETVKALDTDYFSITGAIASLMIWIGTGVVLVISSLVLLIA
                     KIALTLLLTTAPVFIYCLTFPFLRQTFNNWVQNVLSSILTVMFASLVINFGLEFYNDI
                     LNQLTTNVDNASLMTTGAIAVVCGIVAGAIVLVSAKLAQSISGAGIDAAIQGAAAMGI
                     GGSILAASKTAGTAKDIGTGAYEGASGKNWKERESKGVTPAIAYGSGRIGRAAIQKVI
                     ASNSRSEQSIPTARN"
     gene            1754..2461
                     /locus_tag="ACHKTW_005018"
     CDS             1754..2461
                     /locus_tag="ACHKTW_005018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016537130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="HFO6465880.1"
                     /translation="MNLNKYLLVIAIAYSSSVFSAGIPVVDTVGNAQELAHWTEKLKQ
                     WTETVQHYKSQLQAYKDQLATSTGLRDIQGLVSQGKSLKNDIISLQKQGISLDSLLTS
                     SNAPSGTLDTLYKKFKDFDICDEKQAESYINICKQETVNKAWAIEQTADVQEKISDAL
                     NDISDLTDRMANAKDIKESQDLANAIQAKSIQLNILSQQWEINMKSSEQRDKLLAEKR
                     RKAFRESQITAPVPQFN"
     gene            3367..3861
                     /locus_tag="ACHKTW_005019"
     CDS             3367..3861
                     /locus_tag="ACHKTW_005019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001565850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6465881.1"
                     /translation="MNTSELAFKKVSKLHQQTTVDLDSGAIKQETTRETFSVPAEPPY
                     IKLYIADIAAIHGLSKSQNDVLFQLAMLIGWDGIVSVSKSRFEKTIKPRIGIEYQSFK
                     NIVAKLVEKELFFRSGRGELEANPFIFARGDWPSIYERRKRIELTLVYDEDGRHLKSN
                     IYNE"
     gene            3934..>4217
                     /locus_tag="ACHKTW_005020"
     CDS             3934..>4217
                     /locus_tag="ACHKTW_005020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6465882.1"
                     /translation="MAGRIVPESSGEKNSFFRLLLDLENRGYFIRGVYAEHQNVRGRD
                     IHLKLISSSGDIKYYAVKKFRTTAPHEVTYYAIDKYRRWLRQYKRQPVKS"
BASE COUNT         1301 a          852 c          924 g         1140 t
ORIGIN      
        1 aaaaaaaata cacgggttcg gcattcgagc ccgttttcac acaaaaaagg aaatgttatg
       61 aagagtctta agtacatcct cataggaact atctatctga cggctgcggt ttgcaacgca
      121 gaaccgcaag ttaaaaccgc agaatggtat atgcagcacc atgatgaggt cttatctaaa
      181 tataaagaat gtaaggctgc acaagataag tcttatggcg gtactggata ttcagatgat
      241 tgtaaaaacg tattaaaagc atctctcaga attaaagacc cggattttat taaatcacta
      301 aataaaatca gacttgagag tattaatgag attttaaata agtaaggtga ttgtatggct
      361 ggcttttttg aaaaaatgaa tgaaaagatt gatagtctgt tgactgcaac atcagaaggt
      421 atgtcaggca aatactctga cagtatttca ttaattgctg cttctgctct ggttctgtat
      481 ggagtaatcc ttgcgtttta tataatggga ggaaaaaaga aaatagaaga tgtaggttca
      541 acttttttcc acgcaatgat tatttatgtt tttgtgacta atgccggagg atatttaaat
      601 attttaagtg acggtataaa agggcttaat gatggtcttg ctactgttgg cggtgctggc
      661 agttccgcat ggagcttgct tgataaacaa tgggaagtaa cacaacgact agcggagaca
      721 gtgaaagctt tagatacaga ctatttttcc ataacaggag ccattgcttc attaatgata
      781 tggattggta caggagttgt tttggttata tcaagtctgg ttttattaat tgctaaaatc
      841 gcgcttacac ttcttttaac cactgcacct gtttttattt actgtcttac attcccattt
      901 ttacggcaga ccttcaataa ctgggtgcaa aacgttttat catccattct aacggtaatg
      961 tttgcctcac tggtgattaa ttttggttta gaattttata acgacatact taaccagtta
     1021 acgacaaacg tagataacgc aagcctcatg acaaccggcg ctatagcggt tgtttgcgga
     1081 attgtcgccg gtgctattgt ccttgtgtcc gcaaaacttg cgcaaagcat ttctggtgca
     1141 ggcattgatg cagctattca gggtgctgcc gctatgggga taggcgggag tattttagct
     1201 gccagcaaaa cagcaggaac ggcaaaagat attggcaccg gagcttatga aggggctagc
     1261 gggaaaaact ggaaggagcg cgaatcaaaa ggcgttacac ctgcaatcgc atatggtagt
     1321 gggcgtatcg gcagagcagc tattcagaaa gtgatagcca gcaattcacg cagcgaacag
     1381 agcatcccaa cagcacgaaa ttaaacaaga cgcaggatag gcatagccac tacaaatgtg
     1441 cacatttgag tatctacgcc agaaagtttt ttaaactttc tcctgccgct gcacggggcc
     1501 attttaaaaa ccaaaaaaca aataacttaa gcatgaaaat catattagtt atgctaactg
     1561 taattgctac agtaagtatc tttctgtttt ctatgtatct cacggttgtt tttcttcaca
     1621 gaaaagtttc accgtacata gaccatattt ttaaaaagta tatttttact gaaaaaccat
     1681 ccttaaggcg aataatcatt atctattgtt cgatatttat ttttttacgt tttatagtat
     1741 taaggagcgt tcaatgaatt taaataaata cttattggtc attgctattg cttattcatc
     1801 atctgttttt tctgccggga ttcctgtagt tgatactgtt ggtaacgcac aggaactggc
     1861 gcactggaca gaaaagctga aacaatggac agagacagta cagcattata aaagccagtt
     1921 acaggcttat aaagatcaac tggcaacgtc tacaggtctt agagatatac aaggtttagt
     1981 atctcagggg aaaagcctta agaatgatat tatcagcctt cagaaacaag gaatcagtct
     2041 ggacagtctt ttaacatcga gcaacgcacc atcaggaact ctcgatacac tgtataaaaa
     2101 gtttaaagat tttgatatct gcgatgaaaa acaagcagaa tcatatatca atatttgcaa
     2161 acaggagaca gtaaacaaag cctgggcgat agaacaaaca gcagacgtac aggagaaaat
     2221 aagtgatgcc ctcaatgata tctcagatct taccgatcgt atggcgaacg ccaaagatat
     2281 aaaagaaagt caggacttag ctaatgcgat ccaggctaaa agcatccagc ttaatattct
     2341 ctctcaacaa tgggaaatta atatgaaatc atcagaacag cgtgataaac ttctagcaga
     2401 gaaacgccgg aaggctttca gggaaagtca gattacagca cctgtaccgc aatttaatta
     2461 gcatcggggt tatccgagtt aaaagagcgg gtggtaataa atatctttaa attaattata
     2521 ttaaaaccaa ctgtagttca ccgggcaaac gttgcgaaat gacgtcattt tgcggcgttt
     2581 gccctatcct gcatcggagt taaaccacga agatttttat tccccgttct tcagaacgac
     2641 cgagccgcca cggttccggc agcgccttga gcgcaaccgg aaaccgtctg gggagaaccc
     2701 cagcccccca tcgctgtaac accgacggtc tacgaccacc taccaaccga gtagctgcct
     2761 gtaccgtgca aatgggcttt gcagggggct ggcgcggttc ctggcgacct gaaagcccct
     2821 gattaggggt atcggcgcgc ctgacctcct gagagcctct gtaagcgttt ttcgcgcctt
     2881 ccatgccctg acgggcattt aaaacccgtt tcgtgcgtct gagagcttct gagcgcgttt
     2941 gaaggcctat ctgacgactc ctccccgaaa gaacaacagc ggaattaccg acaacatgcg
     3001 agaggtggca ccgctcgccg cagccccagc gaccgaacgt agtgagcgag tgggcgagga
     3061 agcggaagag aacaggtcga aaaataagca attacgccct aatttaagcc ctgatttaag
     3121 ccctcgcgca cgcgttaaaa taaagatata gatctggcaa accagataca gcaagggcta
     3181 gcggtgagaa tcgtactcac ctcgggtact aatcgtactc acctcgggta ctaatcgtac
     3241 tcacctcggg tactaatcgt actcacctcg ggtactaatc gtaattgaat taaagtactt
     3301 gcaaataaca ttctttagta cttaaatacg agtactttga gcatatttaa accacctgga
     3361 gatgttgtga acacatcaga actcgctttc aagaaggtct cgaagctcca tcaacagacg
     3421 acagttgatc tcgattctgg ggcaataaag caggagacaa cacgagagac gttctctgtt
     3481 cctgctgaac cgccctacat taagctttat attgctgata ttgctgctat tcatggcctt
     3541 tctaaatcgc agaatgatgt gttgttccag cttgcaatgc ttataggatg ggatggaatt
     3601 gtttctgtat cgaaaagtcg atttgaaaaa acaataaaac caaggatcgg tatcgaatat
     3661 cagtcattca aaaatatcgt tgcaaagctt gttgagaaag agttgttctt ccgttctggt
     3721 cgtggcgagt tagaagctaa ccctttcatt tttgccagag gtgactggcc atctatttac
     3781 gaaagaagaa aaaggatcga gctgacgcta gtttatgatg aagatggaag gcatttaaaa
     3841 tcaaatattt ataacgaata aaaattagcg cggttggcaa gagttcgcac ctcctaccaa
     3901 ccgctaacca aaacaacctg gattggaggc tgtatggctg ggcgcattgt gccagaaagc
     3961 agcggcgaga agaattcttt cttcaggctt ctgctcgatt tggaaaaccg tggttatttt
     4021 atccgtggag tatacgcaga acatcaaaac gttcgtgggc gtgatattca tcttaaactt
     4081 atttcctcaa gcggggatat caagtattac gctgttaaga aattccgtac tactgctcct
     4141 cacgaagtta catattatgc aatcgacaaa tatcgccgtt ggctgagaca atataagcgc
     4201 cagccagtta aatcatg
//
LOCUS       DAZFQD010000236         4066 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.238, whole genome shotgun sequence.
ACCESSION   DAZFQD010000236 DAZFQD010000000
VERSION     DAZFQD010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4066
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.238"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(135..2288)
                     /gene="mobQ"
                     /locus_tag="ACHKTW_005021"
     CDS             complement(135..2288)
                     /gene="mobQ"
                     /locus_tag="ACHKTW_005021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="HFO6465883.1"
                     /translation="MAIYHLSMKIISRSNGYSAVASAAYRSGSLMLDERTGLTHDYTR
                     KSGVAEAVILTPATAPAWCTNRAELWNAVEKAERRKNSQLAREIELAIPRELPQDAAR
                     ETVLAFVRENFVSQGMIADVAFHHMDKTNPHAHIMLTTRAVGPAGFGGKVRDWNDRTH
                     AETWRASWADHANRALANAGYQEEIDHRSYERQGLEKTPGIHLGKSACAMETRGIETE
                     RGEQNRLINRLNLEIQISRTELRNSGLTEPARRVADLLNIGLPDNATSYTLRDIIEAL
                     PEDSNAAWKLTSNAMSMMADMQATRRRWEELNTERKEAMSHAASLKKSSPFSSGFSRI
                     PLMQWAAPEYRSEQGKIQSLSQQMEKLRQHYRKVEKQDIPASQTAFEKQWQQWGESGL
                     AALRSQLLKREEELRRKELEDTERSRAEQLRKNDNAVMDSGVLRAVVTGYGEAPMPGN
                     GEMTCYLLLHNRNGEYTLWGNELEKYRTRIFESVDLMRDRSGYICDRSEIGQHPPLQR
                     VYSSATFEQLLTQVCQTWPQHTRNLRQPKTWPESFCLGEDRQPAMPSLAARKVDFTQG
                     RLPPTLMPVMSSVDRETRQLQLLLVMGVDDSLGGVVRLNGTLYPAFAVPSADNSQLVI
                     SALTDKGLRYAGYGVAVNHDADSHISPAPELMEFHLKTREAPLFAVVNTPEKQPDHLF
                     RSLGFNRTWDEWRREEDARTHATERRPDRGWSQGM"
     gene            2467..2721
                     /gene="mobC"
                     /locus_tag="ACHKTW_005022"
     CDS             2467..2721
                     /gene="mobC"
                     /locus_tag="ACHKTW_005022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase accessory protein MobC"
                     /protein_id="HFO6465884.1"
                     /translation="MSRTLEQKIAEAEARLQRLKAKSRSLDTAQKVIVGAALLAKVRK
                     PEEVQLRAWLLQFLKAEVTRQADVTRILPLINELEALPEQ"
     gene            3252..3959
                     /locus_tag="ACHKTW_005023"
     CDS             3252..3959
                     /locus_tag="ACHKTW_005023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFO6465885.1"
                     /translation="MTSNPLIAYKSNALVEASYKLTLQEQRFLLLCISRLNSGTDVAS
                     PELQKTMTITAAEYFDSFPDMGRKNAEVQLQEAIDRLWDRSIILKDDEKREEFRWIQY
                     RAQYARGEGKAQITFSDAVMPYLTQLKGQFTRVVIKNISNLSRSYSIRIYEILQQFRS
                     TGERIIALDDFKSSLMLDGKYKDFKTLNRDLIKPCVDELNKKSDLAVTVETIKKGRTV
                     VALHFRFKEDKQIKMTI"
BASE COUNT          987 a          994 c         1045 g         1040 t
ORIGIN      
        1 aaaaaaagaa cccccaattt ttgggggcgg aaaaacaaaa ctatgtttgc gctatgtgag
       61 tgtgcttctt gcattcatcc ctgtcgatga acatcgacga acataacacg atgttaaaca
      121 aaacaaaata ttgttcacat tccctgtgac caaccgcgat caggtctcct ctcggtggcg
      181 tgtgttcgtg catcttcctc gcgccgccat tcgtcccagg tacgattgaa acccagcgag
      241 cggaacagat gatccggctg tttttctggt gtgttgacga cagcaaaaag cggtgcctca
      301 cgggttttca gatgaaactc cataagttcc ggcgcaggtg atatgtggct gtctgcatcg
      361 tgatttacgg caacaccata ccctgcatac cgtaacccct tatcggtcag tgcactgatg
      421 accagctggc tgttatcggc tgagggaacg gcaaacgcgg gatacagcgt accattaagc
      481 cggacaacac cgcccagaga gtcatccacg cccatgacca gcaaaagctg aagctggcgg
      541 gtttcccggt cgacagaact catgaccggc atcagcgtcg gtggcagacg tccctgcgta
      601 aaatccacct tccttgctgc cagtgatggc atggctggct ggcggtcttc tccaagacag
      661 aaagactccg gccatgtctt cggttggcgg agattgcggg tatgttgcgg ccatgtctgg
      721 caaacctgcg tcagaagttg ctcaaaggtc gcagaggaat aaacacgttg cagtggtgga
      781 tgttgaccga tttcgcttcg gtcacagata taaccactcc tgtcacgcat taaatcaaca
      841 ctctcaaaga tcctggtgcg gtatttctcc agctcgttcc cccagagcgt atattctccg
      901 ttccggttgt gaagcagaag gtaacaggtc atttccccgt taccaggcat gggagcctcg
      961 ccataccccg taacgactgc tctcagcacc cctgaatcca taacggcatt gtcatttttt
     1021 ctgagctgct ccgcgcgact acgttctgta tcctccagct ccttccggcg caattcctct
     1081 tcacgtttaa gcaactggga tctgagagct gcaaggccgg attcccccca ctgctgccac
     1141 tgtttttcaa atgccgtctg actcgcggga atgtcctgtt tctccacctt ccggtaatgc
     1201 tgtcgcaatt tttccatttg ctggctcaga gactggattt ttccctgctc actacggtat
     1261 tccggtgcag cccattgcat caggggaata cgggaaaaac cggacgaaaa cggcgatgat
     1321 tttttcaggc tggcagcatg gctcatcgct tcctttcgtt cggtgttcag ctcttcccag
     1381 cgtctgcgcg tcgcctgcat atctgccatc atcgacatgg cgttactggt gagtttccat
     1441 gccgcgttac tgtcctccgg caacgcttcg atgatgtcac gtagtgtgta cgaagttgcg
     1501 ttgtcaggaa gcccgatatt cagaaggtca gccacacggc gggcaggctc tgtcagcccg
     1561 ctgttcctga gttcggtgcg ggaaatctgt atttccaggt taagcctgtt aatcagacga
     1621 ttctgttcgc cgcgttctgt ttcgataccg cgtgtttcca tcgcacaggc agactttccg
     1681 aggtgaatac caggcgtttt ctcaagcccc tgtcgctcgt atgaacgatg gtctatctct
     1741 tcctgataac cggcgttcgc cagtgccctg tttgcatggt cagcccatga tgcacgccac
     1801 gtctcggcat gtgtccggtc gttccagtcc ctgacctttc cgccgaaccc agcaggtccg
     1861 acagccctcg tggtcagcat gatgtgcgca tggggatttg ttttatccat gtgatggaac
     1921 gccacatcag caatcatgcc ctgactgaca aagttttctc tgacaaaggc gagaacggtt
     1981 tcccgtgcgg catcctgcgg cagttcacgg gggatagcga gttcaatttc ccgcgcaagt
     2041 tgtgagttct tccggcgttc ggccttctca acggcattcc agagttcggc acggttcgta
     2101 caccaggcag gggctgtcgc gggggtcaga atcacagcct cagccacacc gcttttgcgg
     2161 gtgtagtcgt gagtcagtcc ggtacgctca tcaagcatta gtgaaccaga acgataggca
     2221 gcagaagcaa cggcgctgta gccgttgctt ctggagatga ttttcatact gagatgataa
     2281 atcgccattt ctgccgtaat gcctttttgc agttttcttt cagccccgca gggcgcacac
     2341 aatcgaaacg gagtggagat tgtataagtg cgcacttcgt gatttgccgt gcattatgcc
     2401 agctcagttt ttaaactacc agtgacgcgc taaaatgcga tttttcaccg cgcacggagt
     2461 cacgaaatga gcagaacact cgaacagaag attgccgaag ctgaggccag attacagcgc
     2521 ctgaaggcga agagccggag cctggacaca gcgcagaagg tcattgtggg tgcagcattg
     2581 ctggcaaagg tcagaaagcc ggaggaagtg cagttgcgag cttggttact ccagttcctg
     2641 aaggcagagg tgacacggca ggctgatgtg acgcgaatac tgccgctgat taacgagctg
     2701 gaggcgctgc cagagcagtg attgtatcgt ccgctcgccg cagcccagcg accgaacgtt
     2761 agcgagcgag tgggcgagga agcggaagtg aatcagtcgc caggttgagc acatacgcac
     2821 ggtttttcga caggtgatat gcgctgaacc cgcatgaata ctggatttcc acagggttgg
     2881 agggggtgta ttcggaattt tcaatcctga ttttttgcaa tacattgttt ttatttgatt
     2941 tttattttca gcaccccaag ttatccacag gctcgccgtt aagtttttag atcttgtttt
     3001 taagatcttg tttttaatac ttgttttagg gtcgaaattc ctgattaaaa acaatgcgtt
     3061 acacgtgcat aggtggggga atttaaggta aaggtgatat aatttaaggt gcttgggtga
     3121 ggtaatttaa ggtaaaggtg aggagattta aggtaatatt cacaggaaaa tggggggtga
     3181 aaatataatt gctcaccaat gctaaaggtg ataggttttt atttcacctc atttttggcc
     3241 tggtggttaa aatgacatca aaccctctga tagcctataa gtccaatgcg ctggttgagg
     3301 ccagttacaa gctaacttta caagaacagc gtttcttatt gctctgcata agccgactta
     3361 actccggtac tgacgtagct tcacctgaat tgcaaaagac aatgaccatt acagctgcgg
     3421 aatattttga ttcattccct gatatggggc ggaaaaatgc cgaggttcag ctacaggaag
     3481 cgatcgatcg tttgtgggac aggtcgatca ttctcaagga cgacgagaag cgcgaggagt
     3541 tccgctggat tcagtatcgg gcacagtacg cgaggggaga ggggaaagca caaatcactt
     3601 tttctgacgc ggtaatgcct tatctgacac agttgaaggg gcaattcacc agagtcgtca
     3661 ttaaaaatat ttctaattta tcaagaagtt acagcataag aatatacgaa attctccagc
     3721 aattccgcag tactggggaa cgtattatcg ccctcgatga ttttaaatca tcactgatgc
     3781 ttgatggtaa atataaagat ttcaaaacgt taaacaggga tctaataaaa ccctgtgttg
     3841 acgaactgaa caaaaaaagc gacctggctg taacggttga gacgatcaaa aaggggcgca
     3901 cggttgtagc cctgcatttt cgcttcaagg aagacaaaca gatcaagatg acgatctaga
     3961 atcgatttta aggcacctaa aacgctcact gtcgcgtttt tcttgtgtca ggaatgcgaa
     4021 ggtactattg aagtgaaaag ttgcgtcaaa aacgctctga tgcagc
//
LOCUS       DAZFQD010000237         4049 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.239, whole genome shotgun sequence.
ACCESSION   DAZFQD010000237 DAZFQD010000000
VERSION     DAZFQD010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4049
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.239"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..1035)
                     /locus_tag="ACHKTW_005024"
     CDS             complement(<1..1035)
                     /locus_tag="ACHKTW_005024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6531 domain-containing protein"
                     /protein_id="HFO6465886.1"
                     /translation="MSGKPAARQGDMTQYGGPIVQGSAGVRIGAPTGVACSVCPGGMT
                     SGNPVNPLLGAKVLPGETDLALPGPLPFILSRTYSSYRTRTPAPVGVFGPGWKAPSDI
                     RLQLRDDALVLNDNGGRSIHFEPLLPGEAVYSRSESMWLVRGGKAAQPDGHTLARLWG
                     ALPPDIRLSPHLYLATNSAQGPWWILGWSERVPGAEDVLPAPLPPYRVLTGLADRFGR
                     TLTYRREAAGDLAGEITGVTDGAGREFRLVLTTQAQRAEEARTSSLSSSDSSRPLSAS
                     PFPDTLPGTEYGPDRGIRLSAVWLMHDPAYPESLPGAPLARYTYTEAGELLAVYDRSN
                     TQVRAFTYDAQ"
     gene            complement(1102..3210)
                     /locus_tag="ACHKTW_005025"
     CDS             complement(1102..3210)
                     /locus_tag="ACHKTW_005025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HFO6465887.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLTQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTVWQGDDVQRRVKGVVTWFELGENDKNQMLYSMKVCPPLWRT
                     GLRQNFRIFQNEDIESILATILKENGVTEWSPLFSEPHPSREFCVQYGETDYDFLCRM
                     AAEEGIFFYEEHAQKSTDQSLVLCDTVRYLPESFEIPWNPNTRTEVSTLCISQFRYSA
                     QIRPSSVVTKDYTFKRPGWAGRFDQEGQYQDYQRTQYEVYDYPGRFKGAHGQNFARWQ
                     MDGWRNNAETARGMSRSPEIWPGRRIVLTGHPQANLNREWQVVASELHGEQPQAVPGR
                     QGAGTALENHFAVIPADRTWRPQPLLKPLVDGPQSAVVTGPAGEEIFCDEHGRVRVKF
                     NWDRYNPADQDSSCWIRVAQAWAGTGFGHLAIPRVGQEVIVDFLNGDPDQPIIMGRTY
                     HQENRTPGSLPGTKTQMTIRSKTYKGSGFNELKFDDATGKEQVYIHAQKNMNTEVLNN
                     RTTDVINNHAETIGNNQMIAVTNNQIQTVGVNQIETVGSNQIINVGSVQVETIGLVRA
                     LTVGVAYQTTVGGIMNTSVALMQSSQIGLHKSLRVGLGYDVKVGNNVTFTVGKTKKDD
                     TGQTAIYSAGEHLELCCGKARLVLTKDGQIFLNGTKIHLQGKEQVNGDSLLINWNCAA
                     SKSPPKTPDEKQDTPDMREY"
BASE COUNT          892 a         1183 c         1017 g          957 t
ORIGIN      
        1 ctgcgcgtca tacgtgaaag cgcgcacctg cgtattgctg cggtcatata ccgccagcag
       61 ttcaccggct tccgtatacg tgtaccgcgc cagtggcgca ccgggcaggc tttccgggta
      121 tgccgggtcg tgcatcagcc acaccgccga aagacggatg cccctgtcgg ggccgtattc
      181 ggtaccgggc agtgtgtcgg ggaacggtga ggctgagaga gggcgggaac tgtcagaaga
      241 agatagcgaa gaggtgcggg cctcttccgc ccgctgtgcc tgtgtggtca gtaccagacg
      301 gaactcacgc ccggcaccgt ccgtcacgcc ggtgatttcc ccggccaggt cgcctgcggc
      361 ctcacgccgg tacgtcagcg tccgcccgaa acggtctgcc agcccggtaa gcacccggta
      421 cggcggcagc ggcgctggca gtacgtcctc agcacccggc acccgctctg accaccccag
      481 tatccaccac ggcccctgtg cgctgttggt cgccaggtaa agatgcgggc ttaaccggat
      541 atccggcggc agcgcccccc acagccgcgc cagcgtgtgg ccgtccggct gcgctgcctt
      601 accaccgcgc accagccaca ttgactcgct gcggctgtac accgcctccc ccggcagcag
      661 cggctcaaag tgaatgctcc gcccgccgtt gtcgttgagt accagtgcat catcgcgtag
      721 ctgtaagcgg atatcagaag gcgctttcca gccggggcca aacactccca ccggcgcggg
      781 tgtcctggtc cggtagctgc tgtaggtgcg ggagagaatg aacggcagcg ggccgggcag
      841 cgcaaggtcc gtctcgccgg gcagcacctt cgcccccagc agcggattta ccgggttgcc
      901 cgaagtcatc ccgcccggac acaccgagca cgccacgccg gtgggcgcgc caattcttac
      961 acctgccgaa ccctggacaa taggaccgcc atactgagtc atatctccct gacgcgccgc
     1021 tggttttccg ctcatgattc aattccttct tctgtttatt tttaatgctt gtttgttact
     1081 gaataccgaa caggtacggg atcagtactc tctcatatcc ggcgtatcct gcttttcatc
     1141 aggggtcttc ggtggggatt tcgaggctgc acagttccag ttaatcaaca gtgagtcacc
     1201 attaacctgc tccttaccct gcaaatgaat ttttgtgccg ttgagaaaaa tttgtccgtc
     1261 cttcgtcagc accagccttg ccttaccaca gcagagctcc agatgctcac cggcggagta
     1321 aatcgcggtc tgcccggtat catccttttt cgttttacca acggtgaagg taacgttatt
     1381 tccgactttg acgtcataac ccagcccgac cctcaacgat ttatgcaaac cgatctgcga
     1441 ggactgcatc agagccaccg aagtgttcat aatgccacct accgtcgtct ggtacgccac
     1501 gcccacggtc agcgcacgaa caagtccaat cgtttccacc tgaacagaac ccacgttaat
     1561 gatctggtta ctgcccaccg tctctatctg gttaacgccc accgtctgta tctgattgtt
     1621 ggtaaccgca atcatctgat tgttgccaat ggtctcagca tggttgttta tcacatccgt
     1681 ggtgcggtta ttcagcacct cggtgttcat gttcttctgc gcgtggatgt agacctgttc
     1741 tttccctgtc gcatcgtcaa acttcagttc attaaacccg ctgcccttat aggttttcga
     1801 acgaatggtc atctgcgtct tcgtccccgg caggctgccg ggtgtgcggt tttcctggtg
     1861 gtaggtacgc cccataataa tcggctggtc cggatcgccg ttgaggaagt ccacaatcac
     1921 ctcctgaccc acacgcggta tcgccaggtg gccaaaaccg gtgcctgccc acgcctgtgc
     1981 cacacggatc cagcacgaac tgtcttggtc tgccgggtta taacggtccc agttgaactt
     2041 cacccgcacg cgaccatgtt cgtcgcagaa gatttcctcg cctgccggtc ctgtcacgac
     2101 agcgctctgc gggccgtcga ccagcggttt cagcaacggc tgtggtcgcc atgttctgtc
     2161 tgccgggatc accgcaaaat ggttctccag cgccgtcccc gctccctgcc gtcctggcac
     2221 cgcctgtggc tgttcgccgt gcagttcact tgccaccacc tgccattccc ggttcaggtt
     2281 cgcctgcgga tgccccgtca gcacaattcg tcgtcccggc catatctccg gcgagcgact
     2341 cattccccgc gcggtttctg cattgtttcg ccagccgtcc atctgccagc gggcaaagtt
     2401 ctgcccgtgg gcacccttga aacgtccggg gtagtcatac acttcatact gcgtgcgctg
     2461 gtaatcctgg tactggcctt cctgatcaaa acgccctgcc cagccgggtc gtttaaaggt
     2521 gtagtctttg gtcaccacgg aagaagggcg gatttgtgca ctgtagcgga actggctgat
     2581 gcagagggtg ctcacctcgg tacgggtgtt cggattccag gggatctcaa aggactccgg
     2641 cagataacgc acggtgtcgc acaggaccag gctctggtcg gtactttttt gcgcgtgctc
     2701 ctcataaaag aagatgcctt cctccgccgc catccggcac aggaaatcgt aatcggtttc
     2761 gccgtactgg acacaaaact cacgggaagg atgcggctcg ctgaacagcg gactccactc
     2821 ggtcacaccg ttttccttca ggatcgtagc gaggatgctt tcgatgtcct cattctggaa
     2881 gatacggaag ttctggcgca gccctgtgcg ccacagcggc gggcacacct tcatgctgta
     2941 cagcatctgg tttttgtcgt tctcccccag ttcaaaccag gtcaccacac ctttcacccg
     3001 gcgctgtacg tcatcgccct gccataccgt caggtaggcc attttgtcca gcacctgcgc
     3061 aaattcaagg gaaagaaact gctgactgac cagagaaagg tcgagggaaa aaagtgaaga
     3121 gagtgactgg gtgagatgaa aggaaaccac cgcaaacgcg tccggtggca ggccgtccac
     3181 ttcaagtgtg aaacgtaatc cggttgacat agcaacctcc gtgaaaatga ataataatcc
     3241 gcacatattg catgtacaac atgtcgggcg gatgagataa gcgagtcgac atctggcgtt
     3301 accggggaat atcagaaaaa tgtcatggtt atgtgatgat ttagtctggg attttacttc
     3361 agagaaacgg gtgtctgttt agtcaacaaa ataatcacaa acgcaacgga tgggttctcg
     3421 acgttattca cgacaggctc gtacattaca gcgagtaata aaaaacctga tatgcaaagt
     3481 caaaccaata ttcataatgt ctttttattc acaacaataa ataattcaat aacaatcatt
     3541 gaaatgaatg atgatttgta ttaggaatat acgctaccgc cccaccctga taacatgatc
     3601 tgcacattgc catcttaacg tcgttaaaca ctggcttaat agtggatatt tcaaattagg
     3661 aatttattaa attcacctga tgaatatcat aagcaaatag catagaaaat aatgacaatt
     3721 cccccgaggg gaccatttcc aaagcactgc accaccacca ggtgcccgga tgatcttgct
     3781 tcagtggtaa gagtacctga ttcattgttc ccaaaatgat gttttccccg gccccgccaa
     3841 taatgtgcag ctctcacgtg ataatctatt gattaggtta tattaagaat caatatttaa
     3901 aatacatcca ttttcttgaa taaatcaaca tgtttattta tcaattgctg ttttagctct
     3961 gactcatcaa gctctccata tttgtcctac agataattgc gaataaatac agagaacgcc
     4021 atttatcctt ttagcatacc tgccggata
//
LOCUS       DAZFQD010000238         3998 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.240, whole genome shotgun sequence.
ACCESSION   DAZFQD010000238 DAZFQD010000000
VERSION     DAZFQD010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3998
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.240"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..759
                     /locus_tag="ACHKTW_005026"
     CDS             <1..759
                     /locus_tag="ACHKTW_005026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HFO6465888.1"
                     /translation="GNYEFPQPLHDALDKFQADTGIDIDMHIDAASGGFLAPFVAPDI
                     VWDFRLPRVKSISASGHKFGLAPLGCGWVIWRDEEALPQELVFNVDYLGGQIGTFAIN
                     FSRPAGQVIAQYYEFLRLGREGYTKVQNASYQVAAYLADEIAKLGPYEFICTGRPDEG
                     IPAVCFKLKDGEDPGYTLYDLSERLRLRGWQVPAFTLGGEATDIVVMRIMCRRGFEMD
                     FAELLLEDYKASLKYLSDHPKLQGIAQQNSFKHT"
     gene            915..2450
                     /gene="gadC"
                     /locus_tag="ACHKTW_005027"
     CDS             915..2450
                     /gene="gadC"
                     /locus_tag="ACHKTW_005027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HFO6465889.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            2581..3900
                     /locus_tag="ACHKTW_005028"
     CDS             2581..3900
                     /locus_tag="ACHKTW_005028"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HFO6465890.1"
                     /translation="MDICSRNKKLTIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGIIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
BASE COUNT          947 a         1014 c         1004 g         1033 t
ORIGIN      
        1 ggtaactatg agttcccaca accgctgcac gatgcgctgg ataaattcca ggccgatacc
       61 ggtatcgaca tcgacatgca catcgacgct gccagcggtg gcttcctggc accgttcgtc
      121 gccccggata tcgtctggga cttccgcctg ccgcgtgtga aatcgatcag tgcttcaggc
      181 cataaattcg gtctggctcc gctgggctgc ggctgggtta tctggcgtga cgaagaagcg
      241 ctgccgcagg aactggtgtt caacgttgac tacctgggtg gtcaaattgg tactttcgcc
      301 atcaacttct cccgcccggc gggtcaggta attgcacagt actatgaatt cctgcgcctc
      361 ggtcgtgaag gctacaccaa agtacagaac gcctcttacc aggttgccgc ttatctggcg
      421 gatgaaatcg ccaaactggg gccgtatgag ttcatctgta cgggtcgccc ggacgaaggc
      481 atcccggcgg tttgcttcaa actgaaagat ggtgaagatc cgggatacac cctgtacgac
      541 ctctctgaac gtctgcgtct gcgcggttgg caggttccgg ccttcactct cggcggtgaa
      601 gccaccgaca tcgtggtgat gcgcattatg tgtcgtcgcg gcttcgaaat ggactttgct
      661 gaactgttgc tggaagacta caaagcctcc ctgaaatatc tcagcgatca cccgaaactg
      721 cagggtattg cccagcagaa cagctttaaa catacctgat aacgtttaac ggtaacggtg
      781 tcccgaaacg aacccgtttc gggacaattt ccaaagtctg ttcactggca ttagcaacgg
      841 aaaatattgt tctgaatacg cttcagaaca aaacaggtgc ggttccgaca ggaataccgt
      901 tttaggggga taatatggct acatcagtac agacaggtaa agctaagcag ctcacattac
      961 tcggattctt tgccataacg gcatcgatgg taatggctgt ttatgaatac cctaccttcg
     1021 caacatcggg cttttcatta gtcttcttcc tgctattagg cgggatttta tggtttattc
     1081 ccgtgggact ttgtgctgcg gaaatggcca ccgtcgacgg ctgggaagaa ggtggtgtct
     1141 tcgcctgggt atcaaatact ctggggccga gatggggatt tgcagcgatc tcatttggct
     1201 atctgcaaat cgccattggt tttattccga tgctctattt cgtgttaggg gcactctcct
     1261 acatcctgaa atggccagcg ctgaatgaag accccattac caaaactatt gcagcactca
     1321 tcattctttg ggcgctggca ttaacgcagt ttggtggcac gaaatacacg gcgcgaattg
     1381 ctaaagttgg cttcttcgcc ggtatcctgt tacctgcatt tattttgatc gcattagcgg
     1441 ctatttatct gcactccggt gcccccgttg ctatcgaaat ggattcgaag accttcttcc
     1501 ctgacttctc taaagtgggc acactggttg tatttgttgc cttcattttg agttatatgg
     1561 gcgtagaagc ttccgcaact cacgtcaatg aaatgagcaa ccccgggcgc gactatccac
     1621 tggctatgtt actgctgatg gtggcggcaa tctgcttaag ctctgttggc ggtttatcta
     1681 ttgcgatggt cattccgggt aatgaaatca acctctccgc aggtgtaatg caaaccttta
     1741 ccgttctgat gtcccatgtg gcaccggaaa ttgagtggac ggttcgcgtg atctccgcac
     1801 tgctgttgct gggtgttctg gcggaaatcg cctcctggat tgttggtcct tctcgcggga
     1861 tgtatgtcac agcgcagaaa aacctgctgc cagcggcatt cgctaaaatg aacaaaaatg
     1921 gcgtaccggt aacgctggtc atttcgcagc tggtgattac ttctatcgcg ttgatcatcc
     1981 tcaccaatac cggtggcggt aacaacatgt ccttcctgat cgcactggcg ctgacggtgg
     2041 tgatttatct gtgtgcttat ttcatgctgt ttattggcta cattgtgttg gttcttaaac
     2101 atcctgactt aaaacgcaca tttaatatcc ctggtggtaa aggggtgaaa ttggtcgtgg
     2161 caattgtcgg tctgctgact tcaattatgg cgtttattgt ttccttcctg ccgccggata
     2221 acatccaggg tgattctacc gatatgtatg ttgaattact ggttgttagt ttcctggtgg
     2281 tacttgccct gccctttatt ctctatgctg ttcatgatcg taaaggcaaa gcgaataccg
     2341 gcgtcactct ggagccaatc aacagtcaga acgcaccaaa aggtcacttc ttcctgcacc
     2401 cgcgtgcacg ttcaccacac tatattgtga tgaatgacaa gaaacactaa ccatgtctga
     2461 atggttataa gacaagggag cgataagtca tcgctccctt tttcgtgctt gctgtcgtat
     2521 tgacttaacc gggataagta cgagaatgag cgcacatctg tttaccggaa accagcacat
     2581 atggatatct gctcccgaaa caagaaatta acgattagaa gaccagcgat actagttgca
     2641 ctggcacttt tactgtgtag ttgtaaaagc acgcctccag agtccatggt gacaccacca
     2701 gcaggttcaa agccaccagc cacgacgcaa caatcgtcac aaccgatgcg tggcatctgg
     2761 ctggccacgg tttctcggct cgactggcca ccggtttcct cggttaacat tagtaacccc
     2821 accagccggg cccgtgtaca acaacaggcg atgatcgaca aactggatca tctgcaacgt
     2881 ctcggcataa acacggtctt tttccaggtc aagccggacg gtaccgccct gtggccatcg
     2941 aaaattttgc cgtggtccga tcttatgacc ggtaagattg gtgaaaatcc gggttacgat
     3001 ccgctgcaat ttatgctcga cgaagcccac aagcgtggga tgaaagtaca cgcctggttt
     3061 aacccctatc gcgtatcggt taatacgaag cccggtatta tcagggaatt gaatagcact
     3121 ctgtctcaac aaccggcgag cgtctatgtg caacaccgtg actggatcag aacgtctggc
     3181 gatcgctttg tcctcgaccc gggcatccct gaggttcagg actggatcac atcaatagtc
     3241 gctgaagtgg tttcccgcta tccggtagat ggcgtgcagt ttgacgacta tttctatact
     3301 gaatcaccgg gttcacggct aaatgataac gaaacgtacc gtaaatacgg aggcgcattt
     3361 gcgtcaaaag cagactggcg gcgcaacaat actcagcagt taattgcaaa ggtatcacac
     3421 accattaaaa gcattaagcc gggagtcgaa tttggcgtta gcccggcagg cgtgtggcgt
     3481 aaccgatcac acgatccgct cggttccgat acccgaggcg cggcagccta tgacgaatcc
     3541 tacgctgaca cccgtcgatg ggtggaacaa ggattgctgg attacattgc tccccaaatt
     3601 tactggccgt tctcacggag tgccgcgcgt tatgacgtgt tggcaaaatg gtgggcggat
     3661 gtcgttaaac cgaccaggac ccgcctgtat atcggtatcg ccttctataa agtgggtgaa
     3721 ccttcaaaga tagagccaga ctggatgatt aacggcggcg taccggaact gaaaaagcag
     3781 ctcgatctta acgatgcggt accagaaatt agcggcacca tcttgttccg tgaggactat
     3841 ctgaataaac cgcagactca acaagcggtc agctatctgc aaagtcgttg gggcagttaa
     3901 actcccatgc ttactttgta acaagccggt gctttaccca ccggcgtgtt acaccttgtg
     3961 aaatatccca ctagtaatca tgcttacata agtcaaat
//
LOCUS       DAZFQD010000239         3976 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.241, whole genome shotgun sequence.
ACCESSION   DAZFQD010000239 DAZFQD010000000
VERSION     DAZFQD010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3976
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.241"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(18..215)
                     /gene="yacG"
                     /locus_tag="ACHKTW_005029"
     CDS             complement(18..215)
                     /gene="yacG"
                     /locus_tag="ACHKTW_005029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HFO6465891.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(225..968)
                     /gene="zapD"
                     /locus_tag="ACHKTW_005030"
     CDS             complement(225..968)
                     /gene="zapD"
                     /locus_tag="ACHKTW_005030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HFO6465892.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELDRQQRKLQTWIGVPGVDQSRIEALIQQLKAAGSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDTENGQVPERLDFELACC"
     gene            complement(968..1588)
                     /gene="coaE"
                     /locus_tag="ACHKTW_005031"
     CDS             complement(968..1588)
                     /gene="coaE"
                     /locus_tag="ACHKTW_005031"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308134.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFO6465893.1"
                     /translation="MRYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGANMIAADGTLQRRALRERIFANPEEKNWLNALLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFLSQEKP"
     gene            1613..1657
                     /locus_tag="ACHKTW_005032"
     CDS             1613..1657
                     /locus_tag="ACHKTW_005032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6465894.1"
                     /translation="MIIKTLKMSARKRN"
     gene            1813..2856
                     /gene="guaC"
                     /locus_tag="ACHKTW_005033"
     CDS             1813..2856
                     /gene="guaC"
                     /locus_tag="ACHKTW_005033"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HFO6465895.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHYSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGPVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVQEQENRIFNNL"
     gene            complement(2891..>3976)
                     /gene="hofC"
                     /locus_tag="ACHKTW_005034"
     CDS             complement(2891..>3976)
                     /gene="hofC"
                     /locus_tag="ACHKTW_005034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HFO6465896.1"
                     /translation="VTPLSLKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLAL
                     LAEQHPSKQWQALLQSLAHDLEQGIAFSNALLPWSEVFPPLYQAMIRTGELTGKLDEC
                     CFELARQQKAQRQLTDKVKSALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPL
                     PALTQGIMTLADFSGEWSWLLVLFGFLLAIANKLLMRRPTWLIARQKLLLRIPIMGSL
                     MRGQKLTQIFTILALTQSAGITFLQGVESVRETMRCPYWVQLLTQIQHDISNGHPIWL
                     ALKNAGEFSPLCLQLVRTGEASGSLDLMLDNLAHHHRDNTMALADNLAALLEPALLII
                     TGGIIGTLVVAMYLPIFHLGDAMSGMG"
BASE COUNT          959 a         1020 c         1021 g          976 t
ORIGIN      
        1 gcggcatcag acaaatgtca ctgctttggc tcttcgctcc agtcatcgct ttcggaaaga
       61 tcgccactgc tggggattcg tttttcttca gcagcccatt ctccgaggtc gatcagctga
      121 caacgtttgg agcaaaatgg ccgaaacggg ctgatttcac cccacaccac cgttttcccg
      181 caggttgggc aattcaccgt aatagtttct gacattttca ctccttagca acaggccagt
      241 tcgaaatcca gacgttccgg tacctgtccg ttttcagtgt ccagcggcat aaaacgaatg
      301 gcaaaacggc tcttatgtcc ggaaatttgc ggataaagct gtgaatcgag cgacagattc
      361 aggcgcagca agtcggcatc gccaccgtta tcctgataaa aaccattcag gctggtttgt
      421 ttacggaagg gggccgactg gcgaattaaa tccagcacca tggtaagtgc ctgggtgagc
      481 gggttcaggc tggcaatcca ggtttctacc tggctgtcgc gctgcgcctg gggtagatgc
      541 agccaaatgt gcaatgtagg taaatcaaag ctgcaacagc cgcctgggat gctcagtcgc
      601 tgacgcacca gagcaatcaa acgatcttca cgcagaaatt gcccgatacg cggcgcggaa
      661 attaatacgc tccccgccgc ttttaactgc tgaattaatg cttcaatacg gctctggtcc
      721 acgccaggca cgccaatcca ggtctggagt ttacgttgct gccggtcaag ttctttcaac
      781 agctcagtgc ggacttcgcc gcgctcgaaa acatccagta attcactgac attacggaag
      841 aaatgcagcg cgccagcgtg gtcaacgatg ggtaaattaa cggtgagttg ctgaatcaaa
      901 aactcaatgc gcagccatgt acgcattttt tcatttagtg gatgttcaaa aaggacctgg
      961 gtctgcatta cggtttttcc tgtgagagaa actgcgacgc aagctgcaaa tagtgtgcgt
     1021 gcaggcgggc aacatccgat gcgatagcat ccggtgcgcc gttattatca atgacgtcat
     1081 ctgccacggc aaggcgggct tcgcgcgttg cctgagcagc aaggatttgt tcgacatgct
     1141 cgcgagttac atcatcgcgc tgcatggtgc gcttaagttg cgtttctggg ctgacatcca
     1201 ccaccagcac tcgattcgct tttttataca gtgagttttc taccagcaat ggcacaaccc
     1261 acagtacata gggggaagta gcttgctgga tctggtgttg cgtctcttgc tgaatcagcg
     1321 gatgcagcag ggcgttaagc cagtttttct cttccgggtt ggcgaagatc cgctcgcgca
     1381 aggcccggcg ctgcaatgtt ccatcagcag caatcatgtt agcgccaaag tgatcagcaa
     1441 tggcatgtag cgcaggtgca cctggttcaa ccacctgacg cgcaataata tcggcatcaa
     1501 tgacgttaat tccgagatca gcaaacgcat tggcaacggt actcttgcca ctgccaatgc
     1561 ctcccgttaa ggcaactata tacctcataa tttatattcc cgggaattca tcatgattat
     1621 caaaacgtta aaaatgagtg cacgaaagcg aaattgatga aacgttcgct cactatttac
     1681 caggtaaatt tatgggattg tagcgtaaaa aaagacaatt tcgcagtctt gcgccgcatt
     1741 gattagtgcg tatgatagcg tcactggagt tgcgctctta cccttatagc cattaacccc
     1801 aggaatccgc acatgcgtat tgaagaagat ctgaagttag gttttaaaga cgttctcatc
     1861 cgccctaaac gctccactct taaaagccgt tccgatgttg aactggaacg tcaattcacc
     1921 ttcaaacatt caggtcagag ctggtccggc gtgccgatta tcgccgcaaa tatggacacc
     1981 gtaggtacat tttctatggc ctctgcgctg gcttcttttg atattttgac tgctgtgcat
     2041 aaacactatt ctgtcgaaga gtggcaagcg tttatcaaca attcttccgc tgatgtgctg
     2101 aaacatgtga tggtttctac cggtacgtct gatgcggatt tcgaaaaaac taaacaaatt
     2161 ctcgacctga acccggcatt aaacttcgtt tgtattgatg tggcgaatgg ttattccgaa
     2221 cacttcgtgc agttcgttgc gaaagcgcgt gaagcgtggc cgaccaaaac catttgtgcg
     2281 ggcaacgtag tgactggtga aatgtgtgag gagcttatcc tctccggtgc tgatatcgtt
     2341 aaagttggca ttggcccagg ttctgtttgt acaactcgtg tcaaaacagg cgtcggttat
     2401 ccgcagcttt ctgcggtaat cgaatgtgcc gatgcggcgc acggtctggg cggaatgatc
     2461 gtcagcgacg gtggctgcac cacgccgggc gatgtcgcga aagcctttgg cggcggtgcc
     2521 gatttcgtca tgcttggcgg catgctggcg ggccacgaag agagcggcgg tcgcatcgtt
     2581 gaggagaacg gcgagaaatt tatgctgttc tacggcatga gctccgagtc tgcgatgaaa
     2641 cgtcacgttg gcggcgttgc ggaatatcgc gcagcagaag gtaaaaccgt taagctgccg
     2701 ctgcgaggcc cggttgaaaa taccgcgcgt gatattttgg gcggcttgcg ttcagcttgt
     2761 acatacgttg gggcttcacg cctgaaagaa ctgaccaagc gcaccacgtt tattcgggtg
     2821 caggaacaag aaaaccgcat cttcaacaac ctgtaatctc ccaacgctgg cgtggagcaa
     2881 cacgccagcg ttatcccatc ccactcatcg catcgcctaa atggaaaatt ggcagataca
     2941 tcgccaccac cagcgtaccg ataattcctc ccgttatgat cagcaacgcc ggttccagta
     3001 aggctgcgag gttatccgcc agcgccattg tgttatcccg atgatgatgg gcgaggttgt
     3061 ctaacatgag atccagcgag ccggatgcct ctcccgttct cactaattgc aaacagagcg
     3121 ggctaaactc accggcattt tttagcgcca gccagatggg atgaccgtta ctgatatcgt
     3181 gctggatttg tgtcagaagt tgcacccagt acgggcagcg cattgtttct ctgacgctct
     3241 ctacgccctg taaaaaagta atgcctgcac tttgtgtcag cgccagaatc gtaaagatct
     3301 gcgtgagttt ttgtcccctc atcagtgaac ccataatcgg gattcgtaac aacaatttct
     3361 gccgcgcgat aagccaggta ggtcggcgca tcagcaactt attggctatc gccagcagaa
     3421 agccgaacaa caccagcagc cagctccatt cgccactaaa gtctgccagc gtcatgatcc
     3481 cctgcgttag tgccggtagt ggggtgttga aggtcttata gatagcggca aactccggca
     3541 gaacaaaatg cagcattgcc acaaccacca tgattgccat cgctaaaatg atgatgggat
     3601 aacgtaacgc tgatttcact ttgtcggtca actgacgctg ggctttttgc tgacgcgcca
     3661 gttcaaagca gcattcatcc agcttaccgg tcagttcacc cgtgcggatc atcgcctgat
     3721 agagtggcgg aaatacctct gaccagggta ataaggcatt ggaaaaagca atgccctgtt
     3781 cgagatcgtg cgccagcgat tgcagcaacg cttgccattg cttactggga tgctgttccg
     3841 ccagcagtgc cagcccttca gaaagcgtta acccggcttt gagtagcgtc gccagttgat
     3901 gaatgacttc cgcgctttta tctcctcgcc actgcgcaga attgatggcg attcgcttca
     3961 ggctaagtgg ggtaac
//
LOCUS       DAZFQD010000240         3975 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.242, whole genome shotgun sequence.
ACCESSION   DAZFQD010000240 DAZFQD010000000
VERSION     DAZFQD010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3975
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.242"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            170..670
                     /locus_tag="ACHKTW_005035"
                     /pseudo
     CDS             170..670
                     /locus_tag="ACHKTW_005035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311747.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            672..965
                     /locus_tag="ACHKTW_005036"
     CDS             672..965
                     /locus_tag="ACHKTW_005036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion protein"
                     /protein_id="HFO6465897.1"
                     /translation="MSLVGYNSNIIITIALISLLSACTRENSLKENQNKRSGESQAIV
                     DARQFFSEHPEYISSAELEQKLYREFAQVTTVPAYKEMSMYQLLIISQDRLTH"
     gene            complement(1026..2146)
                     /locus_tag="ACHKTW_005037"
                     /pseudo
     CDS             complement(1026..2146)
                     /locus_tag="ACHKTW_005037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311748.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
     gene            complement(2155..2922)
                     /gene="sctT"
                     /locus_tag="ACHKTW_005038"
     CDS             complement(2155..2922)
                     /gene="sctT"
                     /locus_tag="ACHKTW_005038"
                     /inference="COORDINATES: protein motif:HMM:TIGR01401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     subunit SctT"
                     /protein_id="HFO6465898.1"
                     /translation="MGEAILYQLHSLLAATALGFCRLAPTFYLLPFFASGNIPTVVRH
                     PIIIVVSCALVQHYHYELLNLNEIDIALFAAREIIIGLFIACLLASPFWMFLAIGSFI
                     DNQRGATLSSTLDPATGVDTSELARLFNLFSAAVYLTKGGMNFILETLWQSYNLWPSG
                     NFNFPKLEPLFSYINNIMTHTIVYASPVIAVMLGGEAVLGLLACYASQLNAFAISLTV
                     KSALAFLILIIYFAPILAERVMPLSFFPEQLQLYIEK"
     gene            complement(2924..3184)
                     /locus_tag="ACHKTW_005039"
     CDS             complement(2924..3184)
                     /locus_tag="ACHKTW_005039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscS/YscS/HrcS family type III secretion system
                     export apparatus protein"
                     /protein_id="HFO6465899.1"
                     /translation="MDDIVFAGNRALYLILVMSAGPIAVATFVGLLVGLFQTVTQLQE
                     QTLPFGVKLLCVSICFFLMSGWYGEKLYSFGIEMLNLAFARG"
     gene            complement(3194..3859)
                     /locus_tag="ACHKTW_005040"
     CDS             complement(3194..3859)
                     /locus_tag="ACHKTW_005040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscR/YscR/HrcR family type III secretion system
                     export apparatus protein"
                     /protein_id="HFO6465900.1"
                     /translation="MSNSISLIAILSLFTLLPFIIASGTCFIKFSIVFVIVRNALGLQ
                     QVPSNMTLNGVALLLSMFVMMPVGKEIYNNSQNENLSFNNVASVVNFVETGMSGYKSY
                     LIKYSEPELVSFFEKIQKVNSSEDNEEIIDDDNISIFSLLPAYALSEIKSAFIIGFYI
                     YLPFVVVDLVISSVLLTLGMMMMSPVTISTPIKLILFVAMDGWTMLSKGLILQYFDLS
                     INP"
     gene            complement(3849..>3975)
                     /locus_tag="ACHKTW_005041"
     CDS             complement(3849..>3975)
                     /locus_tag="ACHKTW_005041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HFO6465901.1"
                     /translation="SEKNIEIRVNGALTGYGELVEVDDKLGVEIHSWLSGNNNVK"
BASE COUNT         1357 a          781 c          621 g         1216 t
ORIGIN      
        1 aaaaaattat aatttcgaga actattttaa atgcctctat gcataacttt atttacaatt
       61 taaggtataa accctattcc ctcaatgtta agcaaatata acaatcactc ttattttaaa
      121 ttttacacgt aatatttacc ctaaatgatt ttagagtaca ggtgggcata tgcaagtctt
      181 ttccagtgat gtctacttca ctgtaggcac taatgcatta ttagcatccc aaaaagaata
      241 ttacagcgat ttagttgcgt tggttgatct tggacattcg ttcgttgtta ttgatgagca
      301 tcaacatcga aatctcaaac caaatacaga acctgtaaac atccttttga gcaacaattt
      361 catcaggatc aataagaata ttacattatc tgatttaacg catttcctgg tttcaaatct
      421 tcatacacaa aatgtgtatt ccacacaaga accacttacc catgatgaga tagatatcct
      481 gcggttatgt gtttcttact cgttaaaaca aatagccatt attaaaggaa ttgattataa
      541 aacaatatca tatcacaaaa taagagctct aaataaactt aacataaaag gtactgttta
      601 gttgtttata gcactctgtg aatgggataa acattatttt aaactccaat cttgcgtaag
      661 agaaagttga tatgtcatta gtaggatata atagtaacat cataataacc atcgcgctga
      721 tatcgttgct ctctgcatgc accagagaaa attcattaaa ggagaatcaa aataaaagaa
      781 gtggcgaatc ccaagcgata gtagatgcaa gacagttctt tagcgaacac ccggaatata
      841 tatcatcagc agaactggag caaaagctat atcgcgaatt tgcacaggtt actacagttc
      901 cagcttataa agagatgagt atgtatcaat tgcttatcat ttcacaagat cgtttaactc
      961 attaaataag gatctacacg ctttttgcgt gtagattctt ataaatttct gactacgaca
     1021 gatgtttaat tttttaaatt atcgtcttta ttctcgattt ttgtgtcttc accctcaatg
     1081 aatttatctt ctgaagagag ctgttcatct tgaacaggtt gcccagcatt ctccacatct
     1141 tcaagccaca gcagaagacg taatatttca tctatatttt cgaagctaac ataatcgtag
     1201 cgacgatggg tagcataatt ttttcgtgct aattttttat ctgtgataat tggtattccg
     1261 atttcctttg catatttcct tacgcctaag gccacctcat tagttactct tacagaaatc
     1321 aatggaatag gtgaaagatg tggcttaaag tatatcccta ttgcaatgtg agtagggttg
     1381 gaaatcatca aacgactatt actgacatca gacttcaatt gctcagaaag aatttcctga
     1441 tgcgctctct gcgtttagac ttaatttctg gatttccttc ttgctccttg tattctcttt
     1501 taacttcttg tttatccatt ttcatatctt tcataaataa aaaatattca gcgataaaat
     1561 caaaaattaa gacaattatc atactgccga gacaatacag tatgagaagg aatagcatct
     1621 ctccccaatc tgataataaa gatatgatat ctccatcaag agttttaaaa agcagtgatt
     1681 tatgattact ccaaaatact ttgatcgcca atgcaaaaaa aacaatataa agaattgctt
     1741 taacaaattc ttttacggtt tttaagccaa atatacgctt taagccattt actggattca
     1801 atgaatcaaa cttaaattta acagcttcag ttgctaattg cattttactt tgaaaccagg
     1861 agacaagtat tgttgtgaac atgcaaacca gcaaaaaagg aatgttcgtt ttcaaccaac
     1921 caacccccat agctttaaaa tagtcccaaa tatctggttt agcgttatga tcaaggatgt
     1981 attcaagaat cgacataatg tgatgcacat caaaaacata tccgagatat aaagtaccca
     2041 caagcattat tacagagatg gttaagtctc tactttttag aatttgccct tttttagaag
     2101 catcctggag tttcttttgt gttggctttt cagttttatt ggccatgaaa cactctattt
     2161 ctcaatgtat agttgcaatt gttctgggaa aaaggataac ggcattactc gttcagccag
     2221 tattggggca aaataaataa ttaatattaa aaatgccagc gcacttttga cagtcagtga
     2281 aatggcaaaa gcatttagtt gtgaagcata acatgccagc aatcccagga ctgcctcacc
     2341 gcccagcata acggcaatca cggggcttgc gtaaacgata gtatgagtca ttatgttatt
     2401 aatatatgag aataaaggct ctaatttagg aaaattaaaa ttgcctgaag gccataaatt
     2461 ataactttgc caaagagttt ccagaataaa gttcattcca cctttagtaa gataaaccgc
     2521 agcagagaaa aggttaaata atcttgctaa ttccgaggta tcgacacctg tagccggatc
     2581 caaagtacta cttaatgttg caccgcgttg gttatcgata aagctaccta tagcaaggaa
     2641 catccaaaac ggggatgcta ataagcatgc aataaacagc cctatgataa tttctctggc
     2701 agcgaacaaa gcaatatcga tttcatttaa gttcaataat tcatagtggt aatgctgaac
     2761 taatgcacat gataccacta ttattattgg gtgacgaact actgttggta tattcccact
     2821 ggcgaaaaat ggtaacaaat agaaagttgg tgccaatcgg caaaaaccga gcgctgtagc
     2881 agccagcaga ctatgcaact gatagagtat cgcctccccc atgctatcct ctcgcgaatg
     2941 ctaaattcag catttcgata ccaaatgaat ataatttttc accataccac ccgctcatta
     3001 aaaaaaagca aatacttacg cacaataatt tcacgccaaa tggcaacgtc tgttcttgca
     3061 actgcgttac cgtttgaaaa agccctacca ataaaccaac aaatgtcgca actgcaatag
     3121 gtcctgcaga cataactaaa atcagataca gcgccctatt ccccgcaaaa acaatatcgt
     3181 ccatattttt tcctcaggga ttaatggata ggtcaaaata ttgtaaaatg agcccttttg
     3241 ataacattgt ccaaccgtcc atcgcgacaa acaaaattaa ttttatcggt gtagatatgg
     3301 ttaccggact catcatcatc atacctaacg ttaacagaac gctggatata accagatcca
     3361 ctacgacaaa gggtaaatag atataaaaac ctataataaa tgcacttttt atctcactca
     3421 atgcatatgc gggcagtaac gagaaaattg aaatattatc atcatcgatt atctcttcat
     3481 tgtcttcaga actattcacc ttctgaatct tttcaaaaaa gctaaccaac tcaggttcag
     3541 aatattttat caaatatgat ttataaccac tcatgcctgt ctcaacaaaa ttaacaaccg
     3601 atgctacatt attgaatgaa agattctcat tctgactgtt attatatatt tccttcccaa
     3661 ccggcatcat tacgaacatt gacaatagca aagcaacccc attaagtgtc atatttgatg
     3721 gcacttgctg aagtcccagc gcgtttcgga caataacaaa aacaatcgag aatttaataa
     3781 aacatgtccc ggatgctatt ataaacggca atagtgtaaa taatgataat atcgctatca
     3841 gcgaaatgct atttgacatt attattccct gataaccaag aatgaatttc tacgcccaat
     3901 ttatcatcca cttcaacaag ttcgccatag ccagttagcg caccatttac acgtatctct
     3961 atatttttct cagat
//
LOCUS       DAZFQD010000241         3968 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.243, whole genome shotgun sequence.
ACCESSION   DAZFQD010000241 DAZFQD010000000
VERSION     DAZFQD010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3968
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.243"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            67..1161
                     /gene="ompC"
                     /locus_tag="ACHKTW_005042"
     CDS             67..1161
                     /gene="ompC"
                     /locus_tag="ACHKTW_005042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HFO6465902.1"
                     /translation="MKKMTVALSAVAVAVMFAAGAQAAEVYNKDGNKLDLYGRATALH
                     YFSDDKGNDGDQTYARLGFKGETQINDQLTGFGQWEYQFSGNKTESEGSAGNKTRLAF
                     AGLRFADVGSIDYGRNYGIAYDVGSYTDVLPEFGGDGWTQTDNFMTARTSGVLTYRNT
                     DFFGLVDGLNFAAQYQGKNERDDLQKANGDGYGFSASYEFDGFGLVAAYTKSDRTDKQ
                     VNGGSSVASGKYAEMWGAGLKYDANNLYIATIYSETQNMTAFGDKVNGIADKAENVEA
                     VAQYQFDFGLRPSLAFLQSRGQEVIVGGKNYGDQDLVKYIDVGATYYFNKNMSTYVDY
                     KINLIDENKFTQKAGITTDDIVAVGMTYQF"
     gene            1371..2069
                     /locus_tag="ACHKTW_005043"
     CDS             1371..2069
                     /locus_tag="ACHKTW_005043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO6465903.1"
                     /translation="MQDIHEASLNESVKSEQSPRVVLWEIDLTVQGGERYFFCNELNE
                     KGEPVTWQGRKYEAYPIEGSGFEMNGKGSSARPSLTVSNLFGLVTGMAEDLQSLVGAT
                     VVRRRVYARFLDAVNFVAGNPEADPEQELTDRWVVEQMSSLTAMTASFVLATPTETDG
                     ALFPGRIMLANTCMWDYRGDECGYNGPAVADEFDNPTTDIRKDRCSKYMRGCEMRGMA
                     VNFGGFLSINKLSQ"
     gene            2080..2823
                     /locus_tag="ACHKTW_005044"
     CDS             2080..2823
                     /locus_tag="ACHKTW_005044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFO6465904.1"
                     /translation="MTQTESAILAHARRCAPEESCGFVISTPEGNLYQPCVNISAEPE
                     AYFRIAPEDWLRAEMQGEIVALVHSHPGGLPWLSEADRRLQIKSALPWWLVSRGEIHR
                     FRCVPHLTGRRFEHGVTDCYTLFLDAYHLAGIEMPDFHREDDWWRNGQNLYLDNLAEN
                     GFYRVSPSCAQAGDILLCCFGASVPNHAAIYCGNGELLHHIPEQLSKRERYSEKWQRR
                     THSVWRHRHWSASAFTGIYNDLVAASACM"
     gene            2820..3401
                     /locus_tag="ACHKTW_005045"
     CDS             2820..3401
                     /locus_tag="ACHKTW_005045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFO6465905.1"
                     /translation="MNTAAEAIRALSLQVPGFRCQMNEGWYQIRIAGEDTAPEAVYAR
                     LHEPLSGRAVIHIVPRLAGAGGNGVFQVVLGAAAIVGSFFTAGAMMALWGAALSAGGL
                     TATTMLFSLGASMILGGVAQMLAPKAKTPEYKSTDNGRQNTYFSSLDNMIAQGNPMPV
                     PYGEMLVGSRRISQDISTRDEGSDGKVVVIGRG"
BASE COUNT          947 a          849 c         1200 g          972 t
ORIGIN      
        1 aatattctaa tttttgttca tgacatctga atgcatgaat agttcagttt aaataaggat
       61 taaatcatga aaaaaatgac agtggcactt tctgctgtag cagttgcagt gatgtttgct
      121 gcgggggcgc aggcagcaga agtttataat aaagatggta ataaactgga tctttacggg
      181 cgtgcaaccg ctctgcatta cttctcggat gataaaggta atgacggaga tcagacttac
      241 gctcgtctcg gctttaaagg cgaaacgcag attaatgatc aactgaccgg atttggtcag
      301 tgggaatacc agttctctgg taacaaaacg gaatctgaag gttccgcggg aaataaaacc
      361 cgtctggcat ttgcaggtct gagatttgct gatgttggta gcattgatta cggtcgtaac
      421 tacggtattg cttacgatgt cggatcatat actgacgtac tgccagagtt tggtggtgat
      481 ggctggactc agaccgataa ctttatgact gcccgaactt ccggagttct gacctaccgt
      541 aatacagatt tctttgggct ggttgatggt ctgaattttg cggcgcagta tcagggcaaa
      601 aatgagcgcg atgacctcca gaaggccaat ggtgatgggt atggtttctc agccagctat
      661 gagtttgatg gttttggtct cgtagcggcg tacacgaaat ctgaccgtac tgataagcag
      721 gttaatggag gaagctctgt tgccagtggt aaatatgctg aaatgtgggg ggctggtctg
      781 aaatatgatg ccaataatct gtatattgcg acaatatatt cagagaccca aaatatgacg
      841 gcttttggcg ataaggttaa cggcattgcg gataaggcag aaaacgttga agctgtagcg
      901 caatatcagt ttgattttgg tttacgtcca tcactggcat tcctgcagtc tcgtgggcag
      961 gaggttatag ttggtgggaa aaactatggg gatcaggacc tggttaaata tattgatgtt
     1021 ggagcaacat attacttcaa caaaaatatg tctacctatg ttgattataa aattaacctg
     1081 attgacgaaa acaaatttac tcagaaagcc ggtattacta cagatgatat tgttgctgtg
     1141 ggtatgactt atcagttctg attgttgctg gtaagttaat ggttacaggc cgtctgccct
     1201 tacaggcggt ctgtatcaat gaaaacagtg ttttcattgg tcactgcaat cagcaattgc
     1261 catctggcat atgctgattt aactttctgt tattaccttt attggtttta ttttaaattg
     1321 gacttttatt gttcggggcg cggttgcgcc ccttttttat gggcggatat atgcaggata
     1381 ttcacgaagc aagcctgaac gagtcggtta aatcagagca gtcaccgcgg gtggtgctct
     1441 gggaaatcga cctgactgtg cagggcggtg aacggtattt tttctgcaat gagctgaatg
     1501 aaaaagggga gccggtgacc tggcaggggc ggaagtatga ggcgtacccg attgagggca
     1561 gcggctttga gatgaacgga aagggcagca gtgcccgccc gtcgctgacg gtgtccaatc
     1621 tgttcggcct tgtcaccggg atggcggagg atttgcagag cctggtgggt gccacggtgg
     1681 tccgccgccg ggtgtatgcc cgttttctgg atgcggtgaa ttttgtggcg gggaatccgg
     1741 aggccgaccc ggagcaggag ctgacggacc ggtgggtggt ggagcagatg tcatcgctga
     1801 cagccatgac ggcctcgttt gtgctggcga caccgacgga gacggacggg gcgctgtttc
     1861 ccggtcgcat catgctggcg aacacctgta tgtgggatta ccggggcgat gaatgcgggt
     1921 ataacggtcc ggcagtggcg gatgagttcg acaaccccac cacggatatc cggaaggaca
     1981 gatgcagtaa atacatgcgc gggtgtgaga tgcgcggcat ggcggttaat tttggcggtt
     2041 tcctttccat taataaactt tcgcagtaaa tcctgtttta tgacacagac tgaatcagcg
     2101 attctggcgc atgcccggcg gtgtgcgcct gaggagtcgt gcggcttcgt gataagcacg
     2161 ccggagggca acctgtacca gccgtgcgtg aatatctctg cagagcctga ggcatatttt
     2221 cgtattgcac cggaagactg gctgcgggca gagatgcagg gggagattgt ggcgctggtc
     2281 cacagtcatc ccggtggtct gccctggctg agcgaggctg accggcggct gcagataaaa
     2341 agcgcactgc cctggtggct ggtcagccgg ggggaaattc accggttccg ctgtgtgccg
     2401 cacctgaccg gacggcgctt tgaacacggt gtgacggact gttacaccct gttcctggat
     2461 gcataccatc tggcggggat tgagatgccg gattttcacc gcgaggatga ctggtggcgc
     2521 aacggtcaga acctgtacct ggacaacctg gcggaaaacg gcttttaccg tgtgtctccg
     2581 tcctgtgcac aggcaggcga tatcctgctg tgctgctttg gcgcatcggt gccgaatcat
     2641 gccgccattt actgtggcaa cggtgagctg cttcaccata tacctgaaca actgagtaaa
     2701 cgggagaggt attcagagaa atggcaacga cgaacgcatt ctgtctggcg tcaccgccac
     2761 tggtccgcat ctgccttcac ggggatttac aacgatttgg tcgccgcatc agcctgtatg
     2821 tgaacacggc agcggaggct atccgtgccc tgtcgctgca ggtgccggga ttccgctgtc
     2881 agatgaacga aggctggtac cagatacgta ttgccggtga ggataccgcg ccggaggcgg
     2941 tgtatgcccg tcttcatgaa ccgctgagcg ggagggccgt gattcatatt gtaccgcggc
     3001 tggcaggggc cgggggaaat ggtgtttttc aggtggtgct gggggcagca gccatcgtgg
     3061 gctctttctt caccgccggt gcaatgatgg cgttgtgggg cgcagccctg agtgccggag
     3121 ggctgactgc caccacgatg ctgttctcac tgggtgccag catgatactg ggtggtgtgg
     3181 cccagatgct ggccccgaag gcaaagacgc cggagtacaa aagcacggat aacggcagac
     3241 agaacacgta tttttcgtca ctggacaaca tgattgccca ggggaacccg atgccggtgc
     3301 cttacggtga aatgctggtt ggctcacgac ggatatccca ggacatcagc acccgtgatg
     3361 agggcagtga cgggaaagtg gtggttatcg ggcgaggatg aaaataaaaa aatcccgcag
     3421 tgctcggcgc ctgcgggaag agaacgatgt tgactaacct gttggcgttt tatttttatt
     3481 tacccgcaaa aaatgtaacg cagcgtcatt attgtggcta caggtaattt gcagaaatgt
     3541 gaataaattc agacttttta tccggtagcg ggggagcgga agccaggcgg gtatctgtgg
     3601 aagaagacag ataacatcgt gccgggttta agggataaaa aaatcccgca gaatcagcgg
     3661 agctgcggga gagaacgatg aagattaacg ttatggagtt atttttcagg catcaaaaat
     3721 gtaacgcagc gtcattattg cggctacagg caattgccgg aaatgtgaag aatttcagaa
     3781 attttattcc gtcatgacac aggcaccctc cggggtgcct gttgtttttg ggcataaaca
     3841 gattcagaca tcagacatca gacaggagag ggggacagag tgggtaaagg gggcggcaag
     3901 gggcacacac cgcgtgaggc gaaggacaat ctcaaatcca cgcagatgat gagcgtgatt
     3961 gatgccat
//
LOCUS       DAZFQD010000242         3917 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.244, whole genome shotgun sequence.
ACCESSION   DAZFQD010000242 DAZFQD010000000
VERSION     DAZFQD010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3917
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.244"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            24..632
                     /gene="yibF"
                     /locus_tag="ACHKTW_005046"
     CDS             24..632
                     /gene="yibF"
                     /locus_tag="ACHKTW_005046"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HFO6465906.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            730..2121
                     /gene="selA"
                     /locus_tag="ACHKTW_005047"
     CDS             730..2121
                     /gene="selA"
                     /locus_tag="ACHKTW_005047"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HFO6465907.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRGSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLAAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            2118..>3917
                     /gene="selB"
                     /locus_tag="ACHKTW_005048"
     CDS             2118..>3917
                     /gene="selB"
                     /locus_tag="ACHKTW_005048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HFO6465908.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVNEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPEREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETANAGQRIALNIAGDAEKEQINRGDWLLADVPPEPFTRVIVELQTHT
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQSDADALSVHLERGAVNLADFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRG"
BASE COUNT          856 a         1000 c         1188 g          873 t
ORIGIN      
        1 tttcctcgca caaaggactc ggcatgaaac tcgtcggtag ctacaccagc ccgtttgtac
       61 gcaaactttc tattctgttg ttagaaaagg gcataacttt cgaatttatt aatgaactgc
      121 cctataacgc ggacaacggc gtggcgcaat ttaacccgtt aggaaaagtg ccggtgctgg
      181 tgaccgaaga gggcgaatgt tggtttgatt cgccgatcat cgctgaatat attgaattaa
      241 tgaatgtcgc tccggcgatg ttgccgcgcg atccgctgga gtcgttgcgg gtgcgcaaaa
      301 ttgaggcact ggcggatggc attatggatg ccgggctggt atcggtgcgt gaacaggcgc
      361 gtccagcggc gcagcagtct gaagatgaat tgttacgcca gcgggagaaa atcaaccgca
      421 gtctggatgt gctggaagga tatctggtcg atggcacact caaaaccgat acggtcaatc
      481 tggcgactat cgccattgcc tgtgccgtcg gatatctcaa tttccgccgc gttgcgccag
      541 gctggtgtgt cgatcgccca cacttagtca aactggtcga aaacctgttt agccgcgaaa
      601 gttttgctcg caccgaaccg ccaaaggctt gatgcgcgat atgtcctcct gacccatctc
      661 acgttacaat ccgtggttat gttaaacgcc cttctccgtg tgagagggcc ttgatcagcc
      721 aggtttccta tgacaaccga aacgcgttcc ctctatagtc aacttccggc tattgatcgc
      781 ttattgcgcg atagctcctt cctttctttg cgtgatactt atggtcacac ccgcgtggtg
      841 gaattgttgc gtcagatgct cgacgaagcg cgagaagtga ttcgtggcag ccagacgctg
      901 cctgcgtggt gtgaaaactg ggcgcaagaa gtcgatgccc ggttgacgaa agaagcgcag
      961 agtgcgctgc gtccggtgat caacctgacg ggaaccgtgc tgcataccaa ccttgggcga
     1021 gctttacagg cggaagccgc ggtggaagcc gttgcgcagg ctatgcgttc gccagtgacc
     1081 ctcgagtacg atctggacga cgctggacgc ggacatcgcg atcgtgcgct ggcgcagttg
     1141 ctgtgccgca ttacgggggc ggaagatgcc tgtatcgtta ataacaatgc ggcggcggtg
     1201 ttattgatgt tggcggccac tgccagcggg aaagaggtgg tggtttctcg cggcgaactg
     1261 gtggagattg gcggcgcgtt tcgtattccc gatgttatgc gtcaggcagg ctgcacccta
     1321 cacgaagtag ggaccaccaa ccgcacgcac gcgaatgatt atcgtcaggc ggtgaatgaa
     1381 aataccgcac tgttgatgaa agtacatacc agtaactaca gcattcaggg gttcaccaaa
     1441 gcgatagatg aagcggaact ggtggcgctc ggcaaagagc tggatgttcc cgtagtgact
     1501 gatttaggca gtggctcgct ggtcgatctt agccagtacg gtttgccgaa agagccaatg
     1561 ccgcaggagt tgattgcggc gggcgtcagt ctggtgagtt tctccggcga caagttgtta
     1621 ggcgggccgc aggcaggaat tattgttggt aaaaaagaga tgatcgcccg cctgcaaagc
     1681 cacccgctga agcgtgcatt acgcgcggat aaaatgaccc tcgcggcgct ggaagccacg
     1741 ttgcgtcttt atttacaccc tgaagctctg agtgaaaaat taccgaccct gcgcctgctt
     1801 acccgcagcg cagaggtcat tcaaatccag gcacaacgtt tacaggcccc ccttgccgca
     1861 cattacggcg cggagtttgc ggtacaggtt atgccatgtc tttcgcagat tggcagtggt
     1921 tcgctgccgg ttgatcgcct gccgagcgcg gcattaacgt ttacacccca tgatggacgc
     1981 ggtagccacc ttgagtcatt agccgcccgc tggcgtgaat tgccagtgcc ggtgattggt
     2041 cgtatttatg acggacgatt gtggctggat ttacgctgcc ttgaagatga gcaacggttt
     2101 ttggagatgt tgttgaaatg attattgcga ctgccggaca cgttgaccac ggcaaaacaa
     2161 ccttattgca ggcgattact ggcgtaaatg ctgaccgtct gccggaagaa aaaaagcgcg
     2221 gcatgaccat cgatctcggc tatgcctact ggccgcagcc ggatggtcgc gtgcctggtt
     2281 ttatcgacgt acccggtcat gaaaagtttc tttccaacat gctggcgggc gttggtggta
     2341 tcgatcacgc gctgttggtg gtggcgtgcg atgacggcgt gatggcacag acccgtgagc
     2401 atctggcgat tttgcagctg accggtaacc cgatgctgac agtggcgctg accaaagccg
     2461 atcgcgtgga cgaagcgcgt gttaatgagg ttgaacgcca ggtaaaggag gtgctgcggg
     2521 aatacggttt tgctgaggca aaactgttta tcaccgcagc aaccgaaggt cggggaattg
     2581 atgccctgcg cgagcatctg cttcagttgc cggaacgcga gcacgccagc caacatagtt
     2641 tccgcctcgc tattgaccgc gcatttaccg taaaaggtgc cgggctggtc gtcaccggta
     2701 cggcgttaag cggggaagtg aaggtaggcg attcactctg gctgactggt gtaaataaac
     2761 cgatgcgtgt acgtgcgctg catgcgcaaa accagccaac agaaaccgct aatgccgggc
     2821 agcgtatcgc gcttaacatc gcgggtgatg cggaaaaaga gcagattaac cgtggcgact
     2881 ggctgcttgc cgatgtgccg ccagagccgt tcacacgggt gattgtcgag cttcaaaccc
     2941 atacaccgct gacccagtgg cagccgctgc atattcacca cgccgccagc cacgttacgg
     3001 gacgcgtttc gctgctggaa gataaccttg ccgagctggt gttcgacacg ccgctgtggc
     3061 tggcggataa cgatcgctta gtgctgcgcg atatctcggc ccgcaacacg ctggcggggg
     3121 cgcgtgtggt gatgcttaac ccgccgcgtc gcggtaaacg taagccggaa tatctgcaat
     3181 ggctggcgtc tcttgcacgg gcgcagagcg atgccgatgc gttatctgtt catctggaac
     3241 gcggcgcggt taaccttgcg gatttcgcct gggcgcgcca gctcaacggc gaagggatgc
     3301 gcgaattgct gcaacagcct ggctatattc aggctggtta tagcttgttg aatgcgccgg
     3361 ttgccgctcg ctggcaacgg aaaattctcg acacattagc gacttatcat gagcaacatc
     3421 gcgatgaacc tgggcctggg cgcgaacgtc tgcgacgtat ggcgttgcca atggaagatg
     3481 aagcgctagt actgttgctg attgaaaaga tgcgcgaaag cggcgacatc ctcagtcatc
     3541 acggttggct gcatctgccg gatcacaaag cgggcttcag cgaagagcag caggccatct
     3601 ggcaaaaagc agagccgctg tttggtgacg agccgtggtg ggtgcgtgat ctggcaaaag
     3661 agacgggtac cgacgagcag gcaatgcgcc tgacgctacg ccaggcggcg cagcaaggaa
     3721 taattaccgc gatcgtcaaa gatcgttatt accgtaacga tcggattgtc gaatttgcca
     3781 atatgatccg cgatctcgat caggagtgtg gttcaacctg cgcggcggat ttccgcgatc
     3841 gcttaggcgt aggccgaaag ctggcaattc agattctgga atattttgac cgcattggct
     3901 ttacgcgtcg tcgtgga
//
LOCUS       DAZFQD010000243         3904 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.245, whole genome shotgun sequence.
ACCESSION   DAZFQD010000243 DAZFQD010000000
VERSION     DAZFQD010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3904
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.245"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..348
                     /locus_tag="ACHKTW_005049"
     CDS             <1..348
                     /locus_tag="ACHKTW_005049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001329898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="HFO6465909.1"
                     /translation="KKNRTTTDEKTLSALIRAEKTAEKAAAAKARVTAIIAAERKAAA
                     RAERKARDHELYKAAGLMIVAGLVDSKTGKPKFSAAELVGALAGIAELPRNHPKWQEW
                     ERRGKELLTKDSA"
     gene            complement(609..1628)
                     /locus_tag="ACHKTW_005050"
     CDS             complement(609..1628)
                     /locus_tag="ACHKTW_005050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001271372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFO6465910.1"
                     /translation="MSAALQYFEENLPHRPYHTDDLAFGLRISGKGRALLARYIQQNQ
                     PHAQFWLVFDVDREGAAIDWSDRNAPAPNITVKNPVNGHAHLLYALNIAVRTAPDASV
                     KALKYAAAVERSLCEKLCADVNYSGLICKNPFHLEWQVMEWREDAYTLDELADYLDLS
                     ASERRSIDKHYGMGRNCHLFEMTRKWAYRAIRQGWPAFSQWLDAVIQRVEMYNASLPV
                     PLSPAECRAIGRSIAKYTHRNFTPETFAQYVADTHTPEIQAARGRKGGKANSSENQSD
                     KGKKSAAVRWTANDDKRRRALDMYILGASTEDIAEAVGVSRWTVRRWMDSSVEWLLSK
                     QIIKI"
     gene            complement(2084..3634)
                     /locus_tag="ACHKTW_005051"
     CDS             complement(2084..3634)
                     /locus_tag="ACHKTW_005051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001624483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="HFO6465911.1"
                     /translation="MASYHCTVKTGAKGSALKHADYISRTGEYENYKSREDLEFSSSG
                     NMPSWAKKNPSELWKAADEFERKNGTAYREIEIALPRELTREQRIELVEDFVQKELGD
                     RHAYQYAIHNPPGAIDGKEQPHAHIMFCERINDGIERDPQQFFKRANSKLPERGGAKK
                     ARFGETQQERKSALVELRSRWADVQNEHLARHGHESRVDHRSLKEQGINRTPEVHLGP
                     VQAASLNGEQIVAIQERRSAERELKTARDAANAIQQEQEQKQKIKAVEPVRSARSPEL
                     LLQYRKVMKTVIQGEARLARLGDANPNALKEHKLLQNAKAKKDSLSEWSRRIYEGARY
                     LDKLGRNVVSAQRELRELQEQRNALNGIRGLFRGADKREIDARILEQKSVLETAEHER
                     NEFRNKLQQAESEWDKENAAFKRTEGYKYVGDLDRYREREILAAASLENTRQKVAEEV
                     SVARSQMLSLEPELSISGDEKAQMRHELLAEMAQERQQQEEKALRIQRSWAREASRSN
                     ERDQDMER"
BASE COUNT          938 a         1051 c          932 g          983 t
ORIGIN      
        1 aaaaaaaacc gaacaacgac ggatgaaaaa acgttgtccg cactgatacg ggcagaaaaa
       61 accgcagaga aagcagccgc agcaaaagcc agggtaacgg caatcatagc ggcagaacgg
      121 aaggcagccg caagggcaga acgaaaggcg cgggatcatg aactctacaa ggccgcaggc
      181 ctcatgattg tggcaggact ggtggacagc aaaaccggaa aaccaaagtt tagtgctgcc
      241 gaactggtag gcgcactcgc aggaatagca gagctaccac gcaaccatcc aaaatggcag
      301 gaatgggaaa gacgcgggaa ggagctacta accaaagaca gtgcgtaaga gcctccgttc
      361 acgttccggt tccctggtgc ttcctcgctc attcgctcgc tacgctcggt catgagactg
      421 cggcgagcac aaccacctca atcatgcggt aagccgcacc attgctgggc tgaaaggaga
      481 aaaccgccgg aaagcccgct gctcaaaaag acgccgaaag gcgctgttat ctgatatagg
      541 cttgttcatg cacccaaaat taacagaatt ttaacaaaat aggggggggt atgtgccatt
      601 cttgttaatt aaattttaat tatttgttta ctcagtagcc attctacaga gctatccatc
      661 cagcgtcgaa cagtccaacg gctaacacca accgcctctg caatatcttc tgttgaggct
      721 cccaagatgt acatatcaag cgccctacga cgcttgtcat cgttagcagt ccaccgaaca
      781 gcggcgcttt ttttcccttt gtccgactga ttttctgacg aattggcctt cccgcccttg
      841 cgaccgcgtg ctgcctgaat ttctggcgtg tgcgtatcag ccacatactg tgcgaaagtt
      901 tccggcgtga agttcctgtg cgtgtatttc gcaatgctcc tgccaatagc ccgacattca
      961 gccggagaaa gcggaacggg aagtgatgcg ttgtacattt cgacacgctg gatcacggca
     1021 tcaagccatt gtgagaatgc aggccagccc tgacgaatcg ccctgtaagc ccatttgcgc
     1081 gtcatttcga acaggtggca gtttcgcccc atcccgtaat gtttatcgat gctacgacgc
     1141 tctgaggcgc tcaaatcgag ataatctgcc agttcatcga gggtataggc gtcctcgcgc
     1201 cactccatca cctgccattc caggtggaac ggatttttgc aaatcaggcc gctgtagttc
     1261 acatcagcac aaagtttctc acacagcgaa cgttcaaccg ctgcggcata tttcagcgcc
     1321 ttaaccgagg catcaggcgc ggttctcacg gcgatgttga gtgcgtagag caggtgagcg
     1381 tgtccgttca caggattttt aacggtgatg ttcggcgcgg gggcgttccg gtcgctccag
     1441 tcaatcgcag ccccctcgcg gtcaacgtca aaaaccagcc agaactgcgc atgaggctgg
     1501 ttctgctgga tgtaccgcgc aagaagcgca cgccctttgc cggagatgcg aaggccgaaa
     1561 gcgagatcat ccgtgtgata ggggcggtgg ggtaaatttt cttcgaagta ttgaagcgcg
     1621 gcgctcatgt gttacgattc cttccaggtt atcgaccgac ctcgcccctg tattctcgtg
     1681 acaatcttgg cggagagaca cgaatttcag gggcgtttgt ttttcagtcg cttacactac
     1741 ccccaacaac aaaacctgtc gagccttccc gcaaaaccga tccatttacc ataactcccg
     1801 ttgtgtgcac cccttaaaac gctctcagac gctttctgac gcaaaaaacg caaaaacaca
     1861 aggaaacacc aggaggaacc agaaaaacgc gtcagaattg tttttaggag gctgcataac
     1921 tattcacgaa agcaatgcgc gatctgtttc gagcgttcag aagggcgaac gggtcaaaac
     1981 agcaaacttt ctcatcagaa aacggtgaac gggacgcgga attttttcgc gttccgtgaa
     2041 acgttggcgg ggttcggggc gcagtcccga tattgctaca ttttcaacgc tccatatctt
     2101 gatcacgttc gttactacgt gatgcctccc tggcccagga tcgctgtata cgcagtgcct
     2161 tttcctcctg ttgctgccgt tcctgcgcca tttctgccag taactcatgt cgcatttgag
     2221 ctttttcatc tccagatata ctgagttcag gctccagaga taacatctgg ctacgggcaa
     2281 cggatacttc ttcagcaacc ttttgcctgg tattctctaa agatgccgcg gccagaatct
     2341 cacgttctct gtacctgtcc agatccccaa cgtacttata tccctcggta cgcttaaaag
     2401 cagcgttttc tttgtcccac tcggattcag cttgctgtag cttgttcctg aattcattcc
     2461 tttcatgttc agcggtttcc agcacacttt tttgctccag aatacgcgca tcaatctccc
     2521 ttttgtcagc cccccgaaac aggccacgaa tcccattcaa agcattgcgt tgttcctgta
     2581 gctcccgcag ctccctttgt gcggaaacga cattacgccc cagtttgtct aaataccgtg
     2641 ctccttcata tattcgcctg ctccattcgg aaagagagtc tttttttgct ttcgcattct
     2701 gaagcagctt atgttctttg agcgcattgg gattggcatc ccctaaacga gcaagccttg
     2761 cttctccctg aatgaccgtt ttcataacct tgcggtactg aagcaataac tccggtgaac
     2821 gggctgaacg aacaggctca actgccttga ttttttgctt ttgctcctgt tcctgctgaa
     2881 tggcatttgc ggcatctctg gcggtcttga gttcccgctc agcactcctc cgttcctgga
     2941 ttgcaacaat ctgctcacca ttcagactgg cggcctgaac ggggccaaga tgaacttccg
     3001 gcgtgcgatt gataccctgt tctttcagcg aacgatgatc tacgcgcgac tcgtgaccgt
     3061 gacgcgcaag gtgctcattc tgcacatccg cccagcggga acgtaattca acaagggcag
     3121 attttcgctc ttgttgtgtt tcaccaaagc gtgccttttt tgcgcctcct ctctccggta
     3181 atttgctgtt agcacgttta aaaaactgct gcggatcgcg ttcaatacca tcattaatcc
     3241 gctcgcaaaa catgatatgc gcgtgtggtt gttcctttcc gtcaattgca cctggcggat
     3301 tgtggatcgc gtactggtag gcatggcgat cgccaagttc cttttgcaca aaatcttcaa
     3361 caagttctat ccgttgttcg cgtgtcagtt cacgcggtaa cgcgatctca atttcacggt
     3421 atgccgtccc gttttttcgt tcaaattcat ctgcggcttt ccatagctcg gagggatttt
     3481 tcttcgccca tgatggcatg ttaccggatg acgaaaactc cagatcctcc cgcgatttat
     3541 agttctcgta ttcgccggtt ctggaaatgt aatcagcatg ttttaaggca ctgccttttg
     3601 caccagtttt aacggtgcaa tggtaactag ccatctgcct tttagccctc cgcaggagcg
     3661 cacagtttct cgacgaagga gagaaacgtc taagtgcgca tccctgaatt aatcagggat
     3721 gattttacgg tctcacacgc aggcagacaa ggtgatatta tgcacaataa caactcacat
     3781 taacggcaaa agttcagagg taagaatgac aaatcgtgaa gagtggttaa gtgcaaaaat
     3841 tgcctacatc aacggactga aatcaccgtc agagcagcag agattacttg tgttactggc
     3901 agag
//
LOCUS       DAZFQD010000244         3862 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.246, whole genome shotgun sequence.
ACCESSION   DAZFQD010000244 DAZFQD010000000
VERSION     DAZFQD010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3862
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.246"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(862..1089)
                     /locus_tag="ACHKTW_005052"
     CDS             complement(862..1089)
                     /locus_tag="ACHKTW_005052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6465912.1"
                     /translation="MSVDVIKQIQSIQDKGIIIYSKYRANEFDRDDVYRESYTLIIEF
                     NHLIAEHAVHDEKLINHTVCILNELRSIAIN"
     gene            complement(1481..1675)
                     /locus_tag="ACHKTW_005053"
     CDS             complement(1481..1675)
                     /locus_tag="ACHKTW_005053"
                     /inference="COORDINATES: protein motif:HMM:NF033650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HFO6465913.1"
                     /translation="MNQDKAIYLENKYFSPANEAVRLEREGDYIGAWKKWHDALHYAK
                     GENIDWCIARAEYCKKTGRF"
     gene            2671..3669
                     /locus_tag="ACHKTW_005054"
     CDS             2671..3669
                     /locus_tag="ACHKTW_005054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HFO6465914.1"
                     /translation="MKNPELKNLTDYSPEDKPWDERRSFSDDVGGIYLRAAGFEHYGE
                     RVGACSGILRFGWSTNTETGETRLRLRDARFCRVRHCPICQWRRSMMWQARFYQSLPK
                     IVAEYPGARWLFLTLTVRNCSIDALGETLTAMNAGFLNLKRRKEFRGVLGWVRTTEVT
                     RGKDGSAHPHFHTLLMVSPSMLSGREYVKHARWVELWRDCLRVAYDPNVDIRVVKSRK
                     PKDDESLPCAVAEQLRGAVVETLKYSTKPADMLADPEWFLELTRQVHKRRFVATGGAL
                     KNVLQLERETNEDLIMADGMAEGTDDGSRLAFSWVPVVKKYQRDRAKDKRRLSSDD"
     gene            complement(3652..>3862)
                     /locus_tag="ACHKTW_005055"
     CDS             complement(3652..>3862)
                     /locus_tag="ACHKTW_005055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6465915.1"
                     /translation="PTHHKQHPYHRQNRAQNVSDGLNQPKHIKPLPDAKNRVRKRLSA
                     FLESKKLSAVDFHCRDPETRSIIAA"
BASE COUNT          947 a          840 c          989 g         1086 t
ORIGIN      
        1 aaaaaaaagc ccctcaaaag aggggatatc accggaatta ttgtcaatga gaaattgaat
       61 tatggaagtg agcaacttcc cgacagatgc agaatgtttg accgtaattg aaggagacag
      121 gaaggtgctc gcgattgttt taacgtgatt ttttgtagat gacaattttt tgttcgctgt
      181 acatctgatg attttgagcg cgtttctggc gcttgctgct actgccaaac catccccatg
      241 gtcagttgat gccagaaacg cgcaggagtt ttgtagcgcg tctccggtcc ctaatcaatg
      301 aattcccggt cattaacatg cgtttacccg gaaacgcgcc ctgatgttct atcgtattgc
      361 gggaactgtg tcgagggtgt cgggcaaagc cctgaccagg tggtaatcgt atatccgtgc
      421 gtgcgcggtt atacgattac acatcctgtc ccgaaatcag gattgttttt cacgtacaac
      481 tgcggcaaat tcaccccgtt gcggttgcaa cgatgggaat atcaaaacta cgaaaattat
      541 ttagccattt gctacccctc tggccctggc ctccaccagt tccggggggg atttttttgt
      601 tgcctctcat gacgaactac ggcaaatttc tgacgttgca aactgcaaca cataaaacac
      661 acaaaactca caaaactcac aattgccatg tcctcattga cacccccttg ccatataggg
      721 ggattttttc gcctgtcctg attgtctttt gctgatggtt acggcagatt ggaaaagagc
      781 gcatttgtgg tgctctctcc aaaataatat ttttcattac tcaaactctg atggcttccc
      841 cctgtaatcc agggggattt tttaatttat cgctatgctt cgtaattcat tcagaataca
      901 gacagtatga ttaatcagtt tttcatcatg aacggcatgt tcagcaataa gatgattaaa
      961 ctcaataatc agggtgtaac tctccctgta tacatcatcc cggtcaaatt catttgctct
     1021 gtattttgaa taaataataa ttcctttgtc ctgtatgctc tgaatttgtt taattacatc
     1081 aacactcata aaaacaccca ataaaacaga taacagatgc cagaagcaaa catcacaaaa
     1141 cataccaaag acgtcatttt gcggtgtttg ccctatcctg catcagaacg ctaaaaacag
     1201 gaatctcttt atgacgaatc ctccatttca ggatttttta tgtagcgtct actacatgcg
     1261 atccgttggt gagttcgttt ggcgcagacc caatatttag tgcttattag tgctcattag
     1321 tgattattaa tgcgtattag tgcgtattag tgattattga ttgttaagag tccgcggttg
     1381 ctgaaattct ggaggcacgt taccgactgt atacaaaatg ctggcgcatt ttgatacgtc
     1441 gacgtgccaa aggggacgcc cctttgaaat cccgccagaa ttaaaagcgg cctgtctttt
     1501 tacagtattc agctctggct atacaccagt ctatattttc gcctttggca taatgaagcg
     1561 catcatgcca ttttttccat gctccaatgt aatcaccttc acgctccagt ctgacagctt
     1621 cgtttgcggg tgaaaaatat ttattttcta aataaattgc tttgtcctga ttcatactcg
     1681 taatttatat ttttatttga attaaaaaag agcggcctgt cgggttaata catattggca
     1741 tgactgttga tatatcagcc caactgcacg ctcgtaatcg ttttaacgtg attttcgcaa
     1801 catggcaatc cgggttcatc tgtccggtaa tcaataaaaa ccgtgcgaaa aacgggacag
     1861 gatgtgtatt tgtcggaccg cgcacacgca atcagacaaa tgccgcctgg tcaggactgc
     1921 gccccgacac cccgccaacg tctcacgcag ccagattgaa atctgtcagc gttcgccgtt
     1981 ttctgactcg tcaaacagaa aatcgtgggg tcccgcgcct ccggcgtggg ttccgatttt
     2041 gtgattgacg acggtacgag aaaaaatact ggtgatggtg gttggtgtca cgcagtgagg
     2101 ccaaccacct gcaaagcggg tttttaggag gatgggaatt gaaaaatatc tatcagctat
     2161 acgaggaaaa ttgcaacaga gtcggattta aggttcggcg gacatcatgg gggagaactg
     2221 ttgcggttat taaatccatt ggtgggaaga ctcgcgggaa gctttcgggt tatgcgcctt
     2281 actacggtaa tcaaagggtt gtggctgaga ttcatacgcc gagtggtgtt attacggatg
     2341 ttctttcgtg tgctgggtgc taccagtggg agctcgtgga ttgatgcgtt tttaggtggt
     2401 cgccaccttg tatatatact taagggataa atcgacaact cgaaatcatg ctctaggcct
     2461 tgtcacgact ggcctgaacg tggatttgcg tggaaatagg tttgagagta ttttgtcgga
     2521 agtggtcgca ttaaaagcga cccccgcaac gatgggcgtc gttcgggggt gtgaccacct
     2581 gtaggaattc gaattggtga cacgacaaca caggtgatac aggaatgata acacgtaatt
     2641 tcgtctgtcg ctcactaggg aacggcagac atgaaaaatc ctgaacttaa aaatctgacg
     2701 gattattccc cggaggataa accgtgggat gagcgccgga gcttttccga cgatgtgggc
     2761 ggtatttatc tgcgtgctgc cggatttgag cactacggcg aacgtgtcgg ggcttgtagt
     2821 ggtattttgc ggtttgggtg gtcaacaaac acagagaccg gagaaacgcg tctgcggttg
     2881 cgtgatgcgc ggttctgtcg tgtccggcac tgtccgatct gccagtggag gcgctccatg
     2941 atgtggcaag cccgtttcta tcagtccctg ccaaagattg tggcggaata tccaggcgcg
     3001 cgctggttgt ttctcacgct gacggtgcgc aactgttcca ttgatgcgct tggtgagact
     3061 ttgaccgcga tgaatgcggg ttttttgaat ctcaagcgtc gcaaggagtt tcgtggtgtg
     3121 ctgggctggg ttcgtaccac ggaagtaacg cgcggcaagg atggtagcgc acatccccat
     3181 tttcacactc tgctgatggt ttccccctcg atgttgtccg gccgtgagta tgtgaaacat
     3241 gcccgttggg tggaactgtg gcgtgattgt ctgcgggtgg cgtatgaccc gaatgtggat
     3301 atcagggtgg tgaagtcacg gaaaccgaaa gacgatgaat ctttaccttg tgctgttgcg
     3361 gagcagctgc gcggcgcggt ggttgagact ctgaaatatt ccacaaagcc tgctgatatg
     3421 ctggctgatc ctgaatggtt cctcgaacta acacggcagg tacacaagcg tcgttttgtt
     3481 gctacggggg gagcgcttaa gaacgttctg caactggagc gggaaacgaa cgaggattta
     3541 atcatggcgg atggcatggc agagggtaca gatgacggtt cccgtctggc gttttcgtgg
     3601 gtgccggttg tcaaaaaata ccagcgcgat cgggcgaaag ataaacgtcg gctaagcagc
     3661 gatgattgag cgcgtttctg ggtctctgca atggaaatcc acggctgaca gctttttgct
     3721 ctccagaaac gcactcagac gctttctaac gcgatttttt gcatctggca atggttttat
     3781 atgttttggt tgattcaggc cgtcagagac gttttgagcg cgattttgac gatgatatgg
     3841 atgctgtttg tggtgtgtgg gt
//
LOCUS       DAZFQD010000245         3855 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.247, whole genome shotgun sequence.
ACCESSION   DAZFQD010000245 DAZFQD010000000
VERSION     DAZFQD010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3855
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.247"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..76)
                     /gene="ariR"
                     /locus_tag="ACHKTW_005056"
                     /gene_synonym="ymgB"
     CDS             complement(<1..76)
                     /gene="ariR"
                     /locus_tag="ACHKTW_005056"
                     /gene_synonym="ymgB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm/acid-resistance regulator AriR"
                     /protein_id="HFO6465916.1"
                     /translation="MLEDTTIHNAITDKALASYFRSSGN"
     gene            complement(105..377)
                     /gene="ymgA"
                     /locus_tag="ACHKTW_005057"
     CDS             complement(105..377)
                     /gene="ymgA"
                     /locus_tag="ACHKTW_005057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component-system connector protein YmgA"
                     /protein_id="HFO6465917.1"
                     /translation="MKTSDNERIKYEITGQAVIQILRMKINFSLQTLIKQLLVMKSAE
                     EDAFRRDLIDSIIRDFSNSDSGGPNRRTATADNKSMFNGKKINRIH"
     gene            complement(420..656)
                     /gene="ycgZ"
                     /locus_tag="ACHKTW_005058"
     CDS             complement(420..656)
                     /gene="ycgZ"
                     /locus_tag="ACHKTW_005058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein YcgZ"
                     /protein_id="HFO6465918.1"
                     /translation="MHQNSVTLDSAGAITRYFAKANLPTQQETLGEIVTEILKDGRNL
                     SRKSLCAKLLCRLEHATGEEEQKHYNALIGLLFE"
     gene            970..2181
                     /gene="bluF"
                     /locus_tag="ACHKTW_005059"
     CDS             970..2181
                     /gene="bluF"
                     /locus_tag="ACHKTW_005059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="blue light-responsive regulator BluF"
                     /protein_id="HFO6465919.1"
                     /translation="MLTTLIYRSHIRDDEPVKKIEEMVSIANRRNMQSDVTGILLFNG
                     SHFFQLLEGPEEQVKMIYRAICQDPRHYNIVELLCDYAPARRFGKAGMELFDLRLHER
                     DDVLQAVFDKGTSKFQLTYDDRALQFFRTFVLATEQSTYFEIPAEDSWLFIADGSDKE
                     LDSCALSPTINDHFAFHPIVDPLSRRIIAFEAIVQKNEDSPSAIAVGQRKDGEIYTAD
                     LKSKALAFTMAHALELGDKMISINLLPMTLVNEPDAVSFLLNEIKANALVPEQIIVEF
                     TESEVISRFDEFAEAIKSLKAAGISVAIDHFGAGFAGLLLLSRFQPDRIKISQELITN
                     VHKSGPRQAIIQAIIKCCTSLEIQVSAMGVATPEEWMWLESAGIEMFQGDLFAKAKLN
                     GIPSIAWPEKK"
     gene            2386..3117
                     /gene="bluR"
                     /locus_tag="ACHKTW_005060"
     CDS             2386..3117
                     /gene="bluR"
                     /locus_tag="ACHKTW_005060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor BluR"
                     /protein_id="HFO6465920.1"
                     /translation="MAYYSIGDVAERCGINPVTLRAWQRRYGLLKPQRSEGGHRLFDE
                     EDIQRIEEIKRWISNGVPVGKVKALLETTSQDTEDDWSRLQEEMMSILRMANPAKLRA
                     RIISLGREYPVDQLINHVYLPVRQRLVLDHNTSRIMSSMFDGALIEYAATSLFEMRRK
                     PGKEAILMAWNVEERARLWLEAWRLSLSGWHISVLADPIESPRPELFPTQTLIVWTGM
                     APTRRQNELLQHWGEQGYKVIFHAP"
     gene            3338..3742
                     /gene="ycgX"
                     /locus_tag="ACHKTW_005061"
     CDS             3338..3742
                     /gene="ycgX"
                     /locus_tag="ACHKTW_005061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 domain-containing protein"
                     /protein_id="HFO6465921.1"
                     /translation="MDQVVVFQKMFEQVRKEQNFSWFYSELKHHRIAHYIYYLATDNI
                     RIITHDDTVLLLRGTRNLLKVSTTKNPAKIKEAALLHICGKSTFREYCSTLAGAGVFR
                     WVTDVNHNKRSYYAIDNTLLYIEDVENNKPLI"
     gene            3795..>3855
                     /locus_tag="ACHKTW_005062"
     CDS             3795..>3855
                     /locus_tag="ACHKTW_005062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6465922.1"
                     /translation="MGIKVQRLSLWMINTVLLLL"
BASE COUNT         1140 a          799 c          783 g         1133 t
ORIGIN      
        1 aattacccga actgcgaaag taacttgcta acgctttatc agttattgca ttatgaattg
       61 tagtatcttc aagcatggta acctctcatc ttacttatga aattttaatg tattctgttt
      121 attttcttac cattgaacat acttttattg tcagctgtcg ctgttcttcg gtttggccct
      181 cctgaatcac tattgctaaa atcacgaatt atgctgtcaa ttaaatcgcg tcggaaagca
      241 tcttcctcag ccgatttcat tacgagtaat tgcttaataa gagtctgcaa tgaaaaattt
      301 atcttcatgc gcagtatctg gatcaccgcc tggccagtaa tttcatattt tatacgttca
      361 ttatcagatg tcttcataac gacttcatag ctaacctgtt aaataatcag gctgttgcat
      421 tattcaaaaa gcaacccaat tagtgcatta taatgtttct gttcctcttc cccagtcgcg
      481 tgttccagtc ggcaaagaag tttggcacaa agcgactttc gactcagatt acgtccatct
      541 ttcaaaatct cagtcacaat ttccccgaga gtttcctgct gagtaggcaa gttagctttt
      601 gcaaagtaac gagtgattgc tcccgcagaa tctaaagtca ctgaattttg atgcatgcta
      661 cgcctctgtt aaaaatgtta atcaatgtta agtgaggtaa caatagcaaa cttgtacgaa
      721 atatgtgtac agaatgataa attaattttt tgcaattagt gctaatgcat ataaaacaat
      781 gtgttatgat aaaacatatt gcatcattta actgacaatc tatacaagat ttttcgccaa
      841 ctagtgttca actttttcca ttttttatca atgtaaagaa acggttatgt tttggtatta
      901 aaagaaagac acaagaagat atgtctgtta ccgtcttact ctcgcctcac ccattaccct
      961 ggattgttaa tgcttaccac ccttatttat cgtagccata tacgtgacga cgaacctgtc
     1021 aaaaaaatcg aagaaatggt ttcgatagca aatcgcagga acatgcagtc tgacgtaaca
     1081 gggatcttac tgtttaatgg ttctcatttt ttccagcttc tggaaggtcc ggaagaacag
     1141 gttaaaatga tatatcgggc tatatgccag gatccacggc actataatat tgttgagctg
     1201 ctgtgcgatt acgcgcctgc tcgccgtttt ggcaaagcgg gaatggaatt atttgatttg
     1261 cgcctgcacg agcgagatga cgttttacag gccgtattcg acaaaggcac atcaaaattt
     1321 cagctaactt atgatgacag agcgctacaa ttttttcgta cttttgtcct tgcaaccgaa
     1381 caatcaacct atttcgagat ccctgccgaa gactcctggc tttttatcgc tgacggatct
     1441 gataaagaac ttgattcctg tgccctttca ccaactataa acgaccactt tgcctttcat
     1501 cctattgtcg atcccttatc gcggcggata atcgcttttg aagccattgt gcaaaaaaat
     1561 gaagatagcc catcagccat agcggttggg cagcgtaaag acggggaaat ctacacagcg
     1621 gatctcaaaa gtaaggcgct tgcattcacg atggcacacg cacttgagct cggtgataaa
     1681 atgatttcaa tcaatctatt acctatgacc ctggttaacg aacctgacgc agtctctttt
     1741 ttacttaatg aaataaaggc caatgctctg gtgcctgaac aaatcatcgt tgaatttact
     1801 gaaagtgaag tcatatctcg gtttgatgag tttgccgaag cgattaaatc gctaaaggct
     1861 gctggtatca gtgtagcaat tgatcatttt ggcgcaggtt ttgctggttt gttactcctg
     1921 tcacgcttcc agcctgacag aattaaaatc agtcaggaat tgattaccaa tgttcataaa
     1981 agtgggccac ggcaggcaat tattcaggcg atcataaaat gctgtacatc acttgaaatt
     2041 caagtcagtg ctatgggcgt ggcaacacca gaagagtgga tgtggcttga atccgcagga
     2101 attgagatgt ttcagggaga tctgtttgct aaagctaaat tgaatggtat cccttccata
     2161 gcgtggccag agaaaaaata attttcagca cattctttca catgatttca gtaaatcatc
     2221 gtgaaaatga atacgttacg tttaaattta atccacatta aaaataatgt ttcatccaat
     2281 ttagctgaaa tcttatttat actttgtaca attttagcgt atattgtaca aagtattatt
     2341 gggtcgtgta caggcgacgg agatttgtga ccgcaaggag gaattgtggc ttattacagc
     2401 attggtgatg ttgctgaacg ttgcgggatt aatcctgtca ctctccgggc ctggcaacgc
     2461 cgctacggtt tgttaaaacc acagcgcagt gaaggcggac accgactctt tgatgaagaa
     2521 gacatacaac gcatcgaaga gatcaagcgt tggataagta atggcgtccc tgtaggcaaa
     2581 gttaaagcat tactggaaac caccagccag gatacggaag atgactggag ccgcctgcaa
     2641 gaagagatga tgtcaattct tcgcatggct aatcctgcca aactacgcgc gagaattatt
     2701 tcactgggtc gagagtaccc agtagatcaa ttgattaatc atgtttacct tcctgttcgc
     2761 cagcgtctcg tgcttgatca caacacctcc cgcattatga gcagtatgtt tgacggtgca
     2821 ttaattgaat acgcagcaac ctcgcttttc gaaatgcgcc gtaagcccgg taaagaagcc
     2881 attctgatgg cgtggaatgt tgaagagaga gcacgattgt ggctggaagc atggcgttta
     2941 tcattgtcag gatggcacat ttctgttctt gctgatccca ttgaatcgcc gcgcccggaa
     3001 ctgttcccga cgcaaacatt gattgtctgg acaggcatgg caccaacgag aaggcaaaac
     3061 gaacttttac aacattgggg tgagcaaggt tataaagtca tctttcatgc cccctaattt
     3121 cacctttatc ccgcccctaa gacagaatgt tataatctga acccacaaca gcgtttgtac
     3181 cggagctggt ccgttgcaaa atgctcaccg ggagaattca gatattccct ctatcacact
     3241 ctgtacatat cacagccaaa ggacagacca gattgaagtg gtaaaaccga tcgtgcacac
     3301 tacgcagtct cacaactaac aagaggttat tatcctgatg gatcaggtcg ttgtttttca
     3361 aaagatgttt gagcaagtga gaaaagagca aaacttctcc tggttttatt cagaattaaa
     3421 acatcaccgt attgcacatt acatttatta tctggctacg gataacatca gaattattac
     3481 tcacgatgac acggttttgt tattaagagg aaccaggaac ctgttaaaag ttagtacgac
     3541 caagaaccct gctaaaataa aagaggccgc attgcttcat atttgcggaa aatctacatt
     3601 tcgggaatac tgttcaacac tagcaggcgc gggcgttttc cggtgggtta ctgatgttaa
     3661 tcataacaaa cgcagttact acgccattga taatacgctt ttatatattg aagatgtaga
     3721 aaataataaa ccattaatct agctaaagtt ggatgcttaa gaaatgcttc ataattcagt
     3781 aaggcattag cataatggga ataaaagtac agagactatc cctatggatg ataaatactg
     3841 tccttttatt gctac
//
LOCUS       DAZFQD010000246         3773 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.248, whole genome shotgun sequence.
ACCESSION   DAZFQD010000246 DAZFQD010000000
VERSION     DAZFQD010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3773
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.248"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            60..287
                     /locus_tag="ACHKTW_005063"
     CDS             60..287
                     /locus_tag="ACHKTW_005063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020232163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6465923.1"
                     /translation="MACNVFAFSISGGVSERSYKETEKTSAMTTTHSTKLQPSQAILF
                     KMREDAPPLNLTEEITPTYPTKANYLIHPVR"
     gene            complement(364..606)
                     /gene="yfdY"
                     /locus_tag="ACHKTW_005064"
     CDS             complement(364..606)
                     /gene="yfdY"
                     /locus_tag="ACHKTW_005064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HFO6465924.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            959..1879
                     /gene="lpxP"
                     /locus_tag="ACHKTW_005065"
     CDS             959..1879
                     /gene="lpxP"
                     /locus_tag="ACHKTW_005065"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008951 - palmitoleoyl
                     [acyl-carrier-protein]-dependent acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009409 - response to cold [Evidence IEA];
                     GO_process: GO:0036104 - Kdo2-lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HFO6465925.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFINPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            2235..2306
                     /gene="ypdK"
                     /locus_tag="ACHKTW_005066"
     CDS             2235..2306
                     /gene="ypdK"
                     /locus_tag="ACHKTW_005066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HFO6465926.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(2371..3609)
                     /gene="alaC"
                     /locus_tag="ACHKTW_005067"
     CDS             complement(2371..3609)
                     /gene="alaC"
                     /locus_tag="ACHKTW_005067"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HFO6465927.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKRYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENRDRIRQAIRGIKA
                     MFRADGLLPASSKHIHENAE"
BASE COUNT          976 a          948 c          884 g          965 t
ORIGIN      
        1 gataactttc tatgaaagta aacttaatac ttttcagctt atttttattg gtctctatta
       61 tggcatgcaa tgtttttgca ttttccattt ctggtggtgt aagtgagagg agctataaag
      121 agactgaaaa aacatcagcg atgacgacca cacactctac aaaacttcag ccatcacagg
      181 cgattttgtt taagatgaga gaagatgcgc caccattaaa cctcacagaa gaaatcacgc
      241 ctacttatcc gacaaaggcg aattatctta ttcatcctgt gcgatagcgt ctgtcacatg
      301 gcaggtgaac aatcatgtct gatgctgatg tgaacacctg cccaaatgag ccatcctgac
      361 ccactatcgc ctgccatacc ccaccataaa gcgaatcatc aagccacaag ccggggcaag
      421 aaacataaat atccccgtca ctagttcatt accgccagtt aaccacatgg tgaagtaata
      481 aatgagtgca gcgaggatca ccatgccgaa gaaagaagag actgccgata ccacgttcag
      541 tacctgaccg atgtagccgc ttacgcagac aatacacagg gcgagaaaca tccataaata
      601 aatcatagct caacctcctg ccgcgctaaa aaacctgtaa cttcagtcac tttaatagta
      661 agacaatcct taacctctgg caatttttgc taagcatcaa tgaaaacagc atgttaaatg
      721 caagactgtt gtgtacggaa aaatatttac tttgcacgat taataatcag tagctgaaag
      781 cagtcagcga gtgataaagt acagataccg cattcatgct cctcgtggtt atgtcctgac
      841 tagtctttac actctttaca ggaaccattg tcgtacatga tggcccaacc aattgagtgt
      901 accgctgtcg acatggaatc ttctgcgata caacaattcg tatctacaga aggtaactat
      961 gtttccacaa tgcaaatttt cccgcgagtt tctacatcct cgctactggc tcacatggtt
     1021 tgggcttggt gtactctggc tttgggtaca gcttccttat cctgttctct gctttctcgg
     1081 cacgcgtatt ggcgcaatgg cgcgaccatt cctgaaacgt cgtgaatcta tcgcccgtaa
     1141 aaacctggaa ctttgtttcc cgcagcattc tgcggaagaa cgcgagaaga tgattgccga
     1201 aaactttcgt tcactcggca tggcgctggt agaaaccggc atggcatggt tctggcccga
     1261 cagtcgcgta cgtaaatggt ttgatgttga agggttggat aaccttaaac gcgcacaaat
     1321 gcaaaatcgc ggcgtaatgg ttgtcggcgt ccattttatg tcgctggaac tgggcggccg
     1381 cgtgatggga ctgtgccaac caatgatggc tacctatcgt ccacataata atcagctgat
     1441 ggaatgggtg cagacccgtg ggcgcatgcg ctctaacaaa gcgatgatcg gcagaaataa
     1501 tctgcgcggc attgtcggtg cactgaagaa aggtgaagcg gtatggtttg ctcccgatca
     1561 ggattatggt cgtaaaggca gctccttcgc gccgttcttt gcggtggaaa atgtcgccac
     1621 aaccaatggc acctatgttc tctcccgtct ctctggcgca gccatgttga ccgtaacgat
     1681 ggtaagaaaa gcggattaca gcggatatcg tttgttcatc aacccagaga tggaaggcta
     1741 cccgacagat gaaaatcaag ccgctgccta tatgaacaag attatcgaga aagagatcat
     1801 gcgcgcaccg gagcagtacc tctggatcca ccgtcgcttt aaaacgcgcc cggtgggaga
     1861 atcgtcgttg tacatttaac aaactgctct tactctttcc ctcctcaaat cggccataat
     1921 aacctcatgg tttttatggc ttttttctta aggttttttc cttcgctgaa tatctaatta
     1981 ttaccgtcag aaattaaact gtcgccgata tgagacactt gtaagttact aaaagtaaat
     2041 aagaaaatgc ctcttgtctc gtttcagttt taggcgtaca ttagcgccgt ctcgagcagt
     2101 ttagctcaga attctggggt gatgtccacg gtaaaataca gggcttttaa ggtcgctttg
     2161 cccggtgtca actcactgta tcaggtgtaa tgaagtcatt caggcgtaac agtaattacg
     2221 cggagagatg taaagtgaaa tatttcttta tgggcatttc ttttatggtc atcgtttggg
     2281 ccggtacttt tgccctgatg atctaaaagc aaactgtgca atgaaaaaca ggagccatca
     2341 ggctcctgtt tgcatttctg acgacggtgt ttattccgcg ttttcgtgaa tatgtttgct
     2401 gctggcgggt agtaaaccgt cggcacggaa catcgcttta atccctctga tggcctgacg
     2461 aatacggtcg cggttttcaa tcagtgcaaa gcgaacatgc gtatcaccat agtcgccaaa
     2521 gccaatcccc ggcgagacac aaaccttcgc ttcgttaagg agcttcttgg caaattccag
     2581 cgatcccatg gccgcatatg gttccgggat tttcgcccag acatacatcg aagccttcgg
     2641 catttcgacc atccagcccg cttcatgcag ccctttaacc aatacatcac ggcggcgttt
     2701 gtactgttcg gcaatgtcgc gcacgcactg ttgatcgccc tcgagtgccg caatcgccgc
     2761 cacctgcaac ggcgtaaaag tgccgtaatc gtgatagctt ttgattcgcg ccagggcact
     2821 gaccagcgtt ttgttgccga ccataaagcc gatacgccag cccgccatgt tgtagctttt
     2881 cgacagcgta aagaactcga ctgccacatc gcgtgcaccc ggtacctgca taattgacgg
     2941 cgctttccag ccatcgtaga cgatatcggc ataggccagg tcatggacca ccagcacatc
     3001 gtagcgtttc gccagcgcca ctactttttc gaagaactcc agttccacgc attgcgcggt
     3061 tgggtttgac gggaagccga ggatcatcat cttcggtttc ggataacttt cacgaatggc
     3121 gcgctccagt tcgttgaaga aatcgacacc ttccaccagc ggcactgagc gtacctgcgc
     3181 cccggcaatc accgcgccat aaatatggat cgggtaactt ggattcggca ccagcaccgt
     3241 gtcaccatga tccagcgtcg ccagcatcag atgcgccagg ccctctttcg aaccaatagt
     3301 gacgatggct tctgattccg ggtcgatttc aacgtcgtag cgatcctgat accagcggga
     3361 aatggcgcga cgtaaccgcg gaatgccgcg tgaagtggag taaccatgcg tgtccgggcg
     3421 ctgggccaca gtacataatt tttcgacgat atgcggcgga gtcgcaccgt ccgggttacc
     3481 catgctgaaa tcgataatat cttcgccgcg ccgacgcgca gccattttca gttcagcggt
     3541 gatgttaaaa acgtagggcg ggagacgatc aatgcgcgta aagcgacgtt cagggcgagt
     3601 gtcagccata atttcctcag attaacgtta gcgcccggac cgtccgagcg acgctgccac
     3661 aatggtggct gttttgaaaa tagcctgatt aatttctgtc tgtccagcgt tttagaacat
     3721 ttttatcaat ggctttaaat gaggaactcc acttcaaaaa caggaaattt ttc
//
LOCUS       DAZFQD010000247         3661 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.249, whole genome shotgun sequence.
ACCESSION   DAZFQD010000247 DAZFQD010000000
VERSION     DAZFQD010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3661
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.249"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..465
                     /locus_tag="ACHKTW_005068"
     CDS             <1..465
                     /locus_tag="ACHKTW_005068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu-like prophage major head subunit gpT family
                     protein"
                     /protein_id="HFO6465928.1"
                     /translation="VAKTGSVGAQADYSGPAWYLLDCSRAIKPLIYQDRRKAELVAQT
                     KVDEGRAFTDNEFVFGASARRNVGYGFWQMAYMMQSPLTLDALWHGWSAMREFTADGG
                     RKLGIKPTHIVVPTSLEKQAVQLLERELFADGNATVSNEMKGKLELVVADYL"
     gene            509..898
                     /locus_tag="ACHKTW_005069"
     CDS             509..898
                     /locus_tag="ACHKTW_005069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HI1506-related protein"
                     /protein_id="HFO6465929.1"
                     /translation="MNEHHTVAATDDSGLQVSGDNSVTVLAEVRCSRSAFRRAGFLFT
                     RGRQQVEVTPEQLARLEAEPCLTVRILQTPADDAGSVAGVVHAVAGTDLAEAEAEAEA
                     EAEAEAGQDAPRKKTGNKAKQAKKAHA"
     gene            895..1314
                     /locus_tag="ACHKTW_005070"
     CDS             895..1314
                     /locus_tag="ACHKTW_005070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gp436 family protein"
                     /protein_id="HFO6465930.1"
                     /translation="MNYATETDMRARYREDLLRPLLAVPRSDEPDTRKLNRALTDASA
                     LIDSYLSARYTLPLEVIPAVLVQHCCAIAFYYLCDQRASDQARDRYREALAWLKDVMN
                     GNVPVGVDTNGAAPESGDLPQVQSDAAVFGRNQKGFI"
     gene            1311..1871
                     /locus_tag="ACHKTW_005071"
     CDS             1311..1871
                     /locus_tag="ACHKTW_005071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1834 family protein"
                     /protein_id="HFO6465931.1"
                     /translation="MITETEQAYIARIREYFGNELVSVDTHPGDWSDSVLRTMLINAP
                     AIYVAWLGAGEGRTRGRLVSHWVFYVIGDMLNGREASRPGLYQIVARLIAVLNGFRTE
                     KTSPLYFEKAVNGYTETQANSGAVMYALYFSCEEMIAPLTDISSLDDFLRHYETFVEP
                     PGTPPFEAHIDLPGNTTTAVEPPEEL"
     gene            1872..2117
                     /locus_tag="ACHKTW_005072"
     CDS             1872..2117
                     /locus_tag="ACHKTW_005072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2635 domain-containing protein"
                     /protein_id="HFO6465932.1"
                     /translation="MKTIFIKPAPGRLIRDPDTMRPLAQEGEEKTFTPFWCRRLDDGD
                     VIQAEKAAEEAPAVSADATTATEKPVAQPASDKEKPQ"
     gene            2114..3616
                     /locus_tag="ACHKTW_005073"
     CDS             2114..3616
                     /locus_tag="ACHKTW_005073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HFO6465933.1"
                     /translation="MINFDGIGNDRRIPLIGVEFNNSMAVTGTPAQRQCVLLFGQARM
                     KENAVDGTGVLDVPVRITRASQAVELWGRGSMIALMVAEFIAINPDAELYAIAQGAGT
                     GGANAAAFNLTGTAATDGLLCIYIGGRRYALPVKAGQKGKAIIDALVALINKDTDAPF
                     TAVSAVVDGSNAGGLKGSMGVSARFIGECSVHDLRLNYHDGETLPSGLTPQTGFPSSR
                     ARNPDITRSVAGMGDRQYNYVVMPYKDDANLKIISDELLKRWGPAKMSDGVLWLAHTG
                     TFGEVQAFGAKRNDFLATCTSIPKAPEPDYLWAAAVCATCAPSLGADPARPLQTLALP
                     SRMAPAPADRLTREERNSLLWGGIATVSVAAGDMVQIERQVTMYRQNVYGESDPSYLD
                     VETIYTLSYLRYSLRTFITQRFPRHKLADDGTPVRAGQNIVTPEIMKLQLISLGEEWV
                     ELGLVENLDTFKKNLLVERNTSDRNRLDVLCTPDLVNQFRFLAAQIRFIL"
BASE COUNT          800 a          978 c         1099 g          784 t
ORIGIN      
        1 gtggcaaaaa ccggctctgt cggtgcacag gcggattaca gcggtcctgc ctggtatctg
       61 ctcgactgtt cacgcgcaat caaaccgctg atttatcagg atcgccgtaa ggctgaactg
      121 gttgcccaga ccaaagtcga tgaaggtcgc gcattcaccg ataacgaatt tgtgttcggt
      181 gcttccgccc gtcgcaatgt gggctacggc ttctggcaga tggcctacat gatgcagtca
      241 ccgctgacgc tggatgcact gtggcacggc tggtcagcca tgcgcgaatt taccgctgac
      301 ggtggccgca aactcggcat caaacccacc catattgttg tccccacctc gctggaaaaa
      361 caggcggtgc agctgctgga gcgtgaactg ttcgcagacg gaaacgcaac cgtctctaac
      421 gagatgaagg gcaagctgga gctggttgtc gcggattatc tgtaaacggt gagccgggtt
      481 tacggcccgg cccctgcgga gggcaaatat gaatgaacat cacactgtgg cagcaacaga
      541 tgattcaggt cttcaggtca gtggtgacaa ttctgtcacg gtgctggcag aagtgcgttg
      601 cagtcgttcg gcgtttcgtc gtgcggggtt cctgttcacg cgtggacgtc agcaggttga
      661 ggtcaccccg gagcagcttg cccgactgga ggcggagcca tgcctcactg tgcgaatact
      721 acagacgcct gctgatgatg cggggagcgt ggcgggtgtg gttcatgcag tggccggtac
      781 agatttagcc gaagccgaag ccgaagccga agccgaagcc gaagccgaag ccggacagga
      841 tgctccccgg aaaaagaccg gtaacaaagc gaaacaggcg aaaaaggccc acgcatgaat
      901 tacgccactg aaaccgatat gcgggcgcgt taccgcgagg atttgctcag gccgttactg
      961 gcagtgcccc gttcggatga gccggacacg cgcaagctga accgggcgct gacagatgcg
     1021 tcagccctta tcgacagcta tctgtccgcc cgttacacgc tgccgctgga ggttattccg
     1081 gcagttcttg ttcagcactg ttgcgcgatt gccttttatt acctgtgcga tcagagagcc
     1141 tccgatcagg cgcgtgaccg ttaccgcgaa gcgctggcct ggctgaaaga tgtcatgaac
     1201 ggcaatgtgc cggtcggtgt ggatacgaac ggtgcggccc ctgaatccgg ggatttacca
     1261 caggttcagt ctgatgcggc ggtattcggg cgcaaccaga agggcttcat atgattacgg
     1321 aaaccgaaca ggcatacatc gcccgtatcc gtgagtattt cgggaatgaa cttgtgtctg
     1381 ttgacacgca tcccggcgac tggagcgaca gcgtactgcg caccatgctg attaacgctc
     1441 cggcgatcta cgttgcctgg ctgggtgccg gtgaaggccg tacccgtggt cgcctggtca
     1501 gtcactgggt gttctacgtc atcggcgaca tgctcaacgg gcgtgaggcc agccgtcccg
     1561 gactgtatca gattgtggcc cggctgattg ccgtgcttaa cggcttcaga acagaaaaaa
     1621 cctcaccgct ttactttgaa aaggcggtca acggttacac cgaaacccag gcgaacagtg
     1681 gggccgtgat gtatgcgctt tatttctcct gcgaggaaat gatcgcgcca ctgacggata
     1741 tcagctcgct ggacgacttc ctgcgtcatt acgaaacctt tgtggagccg cccggtacgc
     1801 cgccgtttga ggcacatatt gatttgccgg ggaacacgac aacggcggtc gaaccgccgg
     1861 aggaactgta aatgaaaacc atctttatca aacccgcgcc gggacgcctg attcgtgatc
     1921 ccgacacaat gcgcccgctg gcacaggagg gggaggaaaa aaccttcacg ccgttctggt
     1981 gtcggcgtct tgatgacggc gatgtcattc aggctgaaaa agccgctgaa gaggctccgg
     2041 ctgtatctgc ggatgcaacg actgcgacag aaaaaccggt cgcccaaccg gcatccgata
     2101 aggagaaacc gcaatgatta attttgacgg gatcggtaat gaccgccgta taccgctgat
     2161 tggggttgag tttaataact ccatggcggt caccggcacc ccggcacagc gtcagtgtgt
     2221 gctgctgttt ggtcaggcga gaatgaaaga gaatgctgtg gacggtaccg gtgtgctgga
     2281 tgttccggtc aggatcactc gcgcctctca ggctgtggag ctctgggggc gtggctccat
     2341 gatcgctctg atggtggctg aatttattgc catcaatcct gatgcggaac tgtatgctat
     2401 tgcgcagggg gccggaacag gcggggcaaa tgccgccgcc tttaacctta ccggtacggc
     2461 tgcgaccgat ggtttgctct gcatctatat tggtggacga cgctatgcgc tgcccgtaaa
     2521 agccggacaa aaaggaaaag ctatcattga tgcgcttgtt gccctgataa ataaggatac
     2581 tgatgcgcca ttcacagctg tatctgcggt tgttgatggc agtaatgcag gcggtctgaa
     2641 aggaagtatg ggagtgagcg cccgcttcat tggtgaatgc tctgtgcacg atctgcgcct
     2701 gaattatcac gacggtgaaa ccctgccgtc agggctgacg ccccagacag gctttccctc
     2761 ctcaagagcc cgcaatcccg atatcacccg aagtgtggcc ggaatggggg atcgccagta
     2821 caactatgtt gtcatgccct acaaggacga tgcaaacctg aaaatcatca gtgatgaact
     2881 tctgaaacgc tggggaccgg ccaaaatgtc tgacggcgtg ctgtggctgg cacataccgg
     2941 cacgtttggt gaggtgcagg cattcggggc aaaacgcaat gattttctgg cgacctgcac
     3001 atccattccc aaagcccctg aaccggatta cctctgggca gcggcggtct gtgccacctg
     3061 tgcaccctct ctgggagcag atcctgcgcg tccgcttcag acgctggcgc ttccgtcacg
     3121 catggccccg gccccggccg atcgtctgac gcgtgaagag cgtaacagtc tgctgtgggg
     3181 agggattgcc acggtcagtg tggcggccgg ggatatggtt cagattgagc gtcaggtgac
     3241 gatgtatcgc cagaacgtgt acggcgaaag tgatcccagc tatctggatg tggaaaccat
     3301 ctacaccctg tcatatctgc gttattccct gcgcactttc atcactcaac gttttccgcg
     3361 ccataaactg gctgatgacg ggacgcccgt tcgtgccggt cagaatatcg tgacgccgga
     3421 gatcatgaag cttcagttaa tttcgctggg tgaagagtgg gtggaactcg ggctggtcga
     3481 aaatctggac acgtttaaaa agaatctgct tgtggagcgt aatacgtcag atcgaaaccg
     3541 ccttgacgtg ttatgcacgc cggatctggt taaccagttc cgcttcctgg cagcacagat
     3601 ccgtttcatt ttgtgaggta aggcatgagc ggaaaacagt atcagggcac ggccaccatc
     3661 c
//
LOCUS       DAZFQD010000248         3535 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.250, whole genome shotgun sequence.
ACCESSION   DAZFQD010000248 DAZFQD010000000
VERSION     DAZFQD010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3535
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.250"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(1..2139)
                     /locus_tag="ACHKTW_005074"
                     /pseudo
     CDS             complement(1..2139)
                     /locus_tag="ACHKTW_005074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309822.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
     gene            complement(2085..2306)
                     /locus_tag="ACHKTW_005075"
     CDS             complement(2085..2306)
                     /locus_tag="ACHKTW_005075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6465934.1"
                     /translation="MAKIRYLQGTHDARPGDIRDVAQPCAEVLVRLGKAEYITARRPA
                     GQKKKHDAEHGECGTFCDEPEKIRNQDVM"
     gene            complement(2351..>3535)
                     /locus_tag="ACHKTW_005076"
     CDS             complement(2351..>3535)
                     /locus_tag="ACHKTW_005076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFO6465935.1"
                     /translation="LRELEAGKAATAQPVKQAGNGNVAAVASAPVIRVEQKLDKGIGF
                     ARFAKSLAAAKGVRSEALEVARRQYPDDSRLHHVLKSAVGAGTTTDPQWAGSLSEYQE
                     YAQDFIDYLRPQTIIGRFGQGGIPALRQVPFNIRVHAQVSGGAAGWVGEGKAKPLTKF
                     DFESITFSHAKVSAIAVLTEELIRFSSPAADALVRNALAEAVVARLDTDFVDPKKAAV
                     ADVSPASITHDVKGTASTGNPDADAEAAFGQFVAANLQPTGAVWLMSSTNALALSMRK
                     NALGQKEYPDMTLLGGAFQGLPVIVSQYVGDQLVLVNAPDIYLADDGGVAVDMSREAS
                     LEMQSEPTGDSTTPSPVELVSMFQTGSVAIRAERWINWRRRRTAAVAVITGVNYGSAS
                     GG"
BASE COUNT          728 a         1159 c          868 g          780 t
ORIGIN      
        1 caaccaggca ttcccatccc cgcatcccgt gcgttttttc atacgcccgc cagagtccgc
       61 cctggtgtgt ggcatacgtg ccccggggaa aggatttttg atcgtcaatg gcggggagta
      121 tttccagtgc cgtgccatca cgcccgtcct gcggagccgg cagggcactc accgcatcca
      181 gaaccgcctt ctgcagaaca tcgggatcat agtcacgacc atcacgcgga acaggaatat
      241 ggcttaccgc ctccttcacc atctgttcaa gcatcggacg cacatcatcc ggggtgatac
      301 ttttaccgtc cgccggctgc ggaatatttg cgaccgcatc attcaccgcc ttctgcagta
      361 cttccggatc gtaatcacga ccatcacgcg gaacaggaat atggctcact gcctctgtca
      421 ccatctgttc aagcatcgga cgcacatcat ccggggtgat acttttaccg tcctccggct
      481 gcggaatatt tgcgaccgca tcattcaccg ccttctgcag tacttccgga tcgtaatcac
      541 gaccatcacg cggtaccggg atggccccca cagcgtcatc caccatcgcc tgcagaaccg
      601 gacgcacctc atccaccgtc acatgcttct gtaataccac agacagggaa gccagtttct
      661 cttcaaacgc ttgtgcctgc gcggctattt tcccctcaaa tgtgcgctgt aaatccgcca
      721 gcaccgcagc gaattcttcg cccagcgcac ggataatgga cagttcacgt tcattcattt
      781 tttcagaatc cccctgaaca tcgccttcac cgcgtcatgc tctgtttcac tgattgcctt
      841 attaccgtca gatgcgccgt caggcggttg cgctgaggct gttttcccgg tcgacgcaaa
      901 cggatcctca cgggcatcac gacgggacag cgcctccaga ctgtagttct gctgctgaag
      961 atacagtgca tcaccgccgg caaggggcgg caggttctca cgtttacggg cttcattggg
     1021 cgtgagaagc gtatttttca ccgattcacc cagtgttttc atgcgccgtt cgctgtccat
     1081 tctcagcagt gtggtgacat caaactccgt gctctcgttt tcccccgttt ccagcgcctc
     1141 atccagtaac agctcaatgg actcaatcag cgtctgcaga cactgggaat aatactgctg
     1201 ctccagcgcc tccacgttat cactggaggg aggttgtccc acgccaatct tgtaggccgg
     1261 aacacggaac accgaacaga caatttcagc cgtcattttc agttgttcca ccgtctgcgc
     1321 atccaccggt gaaaacgtcg tggggctgta ttttgccccg ttgctcagta tggccgtctt
     1381 cccggcattt tcgcctgtat acccgctgtc ccagttgctc ttcagtattt tcgcattttc
     1441 ttccgtaata ctgccgggga tctcaatcac cccggacggc ctgccgccat ttctgaaaaa
     1501 atacgtcgaa ttttcctgaa tatgatgccc ctgcgtggcg gccagcccgg cggcatacac
     1561 cggcggcaac cctataagcg gatgaaaaaa acagttaaac cggtcgtgga tcacttcccg
     1621 ggcaggcacc gtcaccgcct cagtgatccc acagttccgg tccggcgtga tacggtagaa
     1681 cacctcgccg tcatccgcca ccagaggttc aacccggttc cagtccagaa tacgcagttc
     1741 ttttatctgc ccccgggagt tacggatttt cagcaccacc gtattaccgt gacgcagttt
     1801 tgagttcagc cacagttcaa aaaactggat acgattctgc tgtgcattgg gacgacgaca
     1861 gagacgggca atatccccct gccgtttttc acggcgtatc ccctgtgtat ccgtctgcat
     1921 caggcgcagc cgcattttgg cgatatcctg ggatatcagc gaaatacatg aaaacaccgc
     1981 atgaaaggag aggacacttt ccggatcggc tttcacgccc tgctgccagg caccggcaaa
     2041 gggctcagcc accgcctgaa acaggctggt ccagcccacc tcttttacat cacgtcctga
     2101 tttctgattt tttcgggttc gtcgcaaaag gttccacatt cgccatgctc cgcatcatgt
     2161 ttctttttct gacccgccgg acgtcgagct gttatgtact ccgccttccc caggcgaacc
     2221 agcacctccg cacacggctg tgcgacatcc cggatatccc cgggccgggc atcatgtgta
     2281 ccctgcagat atcggatttt tgccatcagt tactgcggga agctctcacc tcccgccctc
     2341 ctcatcagac tcagccgccg gacgcactgc cgtagttcac accggtgatc accgccaccg
     2401 ccgcggtacg gcgacgacgc cagttgatcc agcgctccgc acggatggcc acactgcctg
     2461 tctggaacat ggaaaccagc tccaccggcg acggcgtggt actgtcgccg gtcggctcag
     2521 actgcatttc cagtgatgcc tcgcgggaca tatccaccgc cacgccgccg tcatccgcca
     2581 gataaatatc cggggcattc accagcacca gctggtcacc cacgtactgg gagacaatca
     2641 ccggcagccc ctggaaggcc cccccaagca gggtcatatc cgggtattct ttctgaccca
     2701 gcgcattttt acgcattgac aacgccaggg cattggtgct ggacatcagc cagaccgcac
     2761 cggtgggctg caggtttgct gccacaaact gaccaaacgc ggcctccgca tccgcatccg
     2821 ggttaccggt tgatgccgtg cccttcacat catgggtgat ggacgccggg gagacatctg
     2881 ccactgcggc ttttttcgga tccacaaagt ctgtatccag acgcgccacc accgcttccg
     2941 ccagcgcatt acggaccagt gcatcagcag ccggactgga aaaacggatc agctcttccg
     3001 tcagtactgc aatggccgac accttcgcat gactgaaggt gatggactca aaatcaaact
     3061 tcgtcagggg ttttgcctta ccctccccca cccagccagc agcaccgccg gacacctggg
     3121 cgtgcacacg gatattgaac ggcacctgac gaagtgcagg gatcccaccc tgaccaaatc
     3181 gcccgataat ggtctgagga cgcaggtaat caataaagtc ctgcgcatat tcctgatatt
     3241 cagacaggct gcctgcccac tgcggatccg tggtggtccc cgcgcccact gccgatttca
     3301 ggacatgatg cagacgactg tcatccggat actgacgacg ggccacttcc agggcttcag
     3361 agcggacacc tttagccgca gccagtgatt tggcaaagcg ggcgaagcca atccccttat
     3421 ccagtttctg ctccacacgg atcaccggcg ctgaagccac cgcggccaca ttcccgttac
     3481 cggcctgttt caccggctgc gccgtggcgg ccttaccggc ttccagttca cgcag
//
LOCUS       DAZFQD010000249         3350 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.251, whole genome shotgun sequence.
ACCESSION   DAZFQD010000249 DAZFQD010000000
VERSION     DAZFQD010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3350
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.251"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..257)
                     /gene="ybgE"
                     /locus_tag="ACHKTW_005077"
     CDS             complement(<1..257)
                     /gene="ybgE"
                     /locus_tag="ACHKTW_005077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HFO6465936.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLAD"
     gene            complement(257..370)
                     /gene="cydX"
                     /locus_tag="ACHKTW_005078"
     CDS             complement(257..370)
                     /gene="cydX"
                     /locus_tag="ACHKTW_005078"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HFO6465937.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            complement(385..1524)
                     /gene="cydB"
                     /locus_tag="ACHKTW_005079"
     CDS             complement(385..1524)
                     /gene="cydB"
                     /locus_tag="ACHKTW_005079"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HFO6465938.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLP
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            complement(1540..3108)
                     /gene="cydA"
                     /locus_tag="ACHKTW_005080"
     CDS             complement(1540..3108)
                     /gene="cydA"
                     /locus_tag="ACHKTW_005080"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HFO6465939.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
BASE COUNT          936 a          921 c          824 g          669 t
ORIGIN      
        1 atatcggcaa gcaatgggca aaaaatccct tgccaaagaa ccttctgcgg acgaaagccc
       61 acgccgtgaa tcacaccagc acagacggcc cacatcagca ataaaccatg ccagatttcc
      121 agttcactgg tcttcgcggc aaaacgagat gggtcccaaa acatacatcc tgctaacaga
      181 agcgccatca cgaaggaaag cgcccgcagg gggcgcttgt ccattaccgc atacaaagtc
      241 gcgataatct tactcatcag atgtcttctt gaccggcttt gcctgattcg acgtgttcaa
      301 gcgccagcgc ggtgattacc ccaaacgaac aggcaagaag cgttcccaga atccatgcga
      361 aataccacat ttttagctcc ttacttagta cagagagtgg gtgttacgtt caatatcttc
      421 tttggtgata cgaccgaaca ttttccagta acaccaggcg gtgtagagca gaatgatcgg
      481 taccagaacc accgcaaccc aggtcatgac gttaagcgtc agctggctgg aagttgcatc
      541 ccacattgtc agacttgcgt tcatcatggt gctggacggc atcacaaacg ggaacattgc
      601 gataccggct gtcaggatga tgcaggccag cgtcagggag gagaacacaa acgcccacgc
      661 ggctttatcc atacgtgcag tcaggatggt cagcagcggc agaaccacac ccagcgccgg
      721 aatagcccac agaattggcg tgttgttgaa gttaaccagc catgcgccag cttcacgaac
      781 cacttcttta ttcagtgggt tagaggctgc gtaatggtcc attgtcgatt tcacgacata
      841 accatcgata ccgtacatca cccatacgcc agccagtgcg aaacagacca gtgtcaccag
      901 cgcagccacc tgagccgttg cacgggtacg caggtgcagt tcgcccacgg tacgcatttg
      961 cagataggtt gcgccctgag taatgatcat ccctacgctc accacgcctg ccagcaggcc
     1021 gaacgggtta agcaactgga agaagttacc ggtgtagtac agacgcagat attcatcaac
     1081 gttgaacggt acgccctgca acaggttacc gaacgctaca ccaattacca gcggcggaac
     1141 gaagctacca atgaagatgc cccagtccca catgttacgc cagcgggttt cttcaatctt
     1201 ggagcggtag tcaaaaccga ccggacggaa gaacaaagac gccagcacga ggatcatcgc
     1261 cacatagaag ccggagaacg cagcggcata gaccatcggc caggcagcaa agagtgcgcc
     1321 gcccgcggtg atcagccaaa cctggttacc gtcccagtgt ggtgcaatgg agttaatcat
     1381 aattcgacgc tcggtgtcgt tacgaccgag gaaacgggtg agcatgccca cccccatgtc
     1441 gaaaccgtca gtgactgcaa aaccaatcag cagaacgcca accagcagcc accagataaa
     1501 acgcaatact tcataatcga tcatttgacg actcctgtct tagcgtgccg gctgagtagt
     1561 cgtggaagac tgctcaaagt gatagcgacc ggttttcagg ctgcttgggc cgaggcgtgc
     1621 aaatttgaac attaagaaca attctgccac caggaacagg gtatacaggc cgcaaatcag
     1681 caccattgag aagatgagat cgcctgcggt cagtgacgag ttcgccacag ctgtcggcag
     1741 cacttcaccg atagcccacg gttggcggcc atattcagcc acgaaccagc ccgcttctac
     1801 agcaatccac ggcagcggaa taccgtacag cgcggcgcgc agaagccatt ttttctcgcc
     1861 aatgcggttg cggatgacac tccagaaaga gagcgcgatg attgccagca gcaggaagcc
     1921 acacgccacc atgatacgga acgcaaagta cagcggcgct acacgcggga tggagtcttt
     1981 ggttgcctgt tgaatctgcg cttcagtcgc atcagccacg tttggcgtat agcgtttcag
     2041 cagcagaccg taaccgaggt ctttcttcat gctattgaac tggtcacgaa ccgcctggtc
     2101 ggtagaacca gaacgcagtt gttcgagcag agagtacgcc ttcatcccgt tacgaatgcg
     2161 ttcttcatgc tgcaccatca gctctttcag gccgataacc ggggtatcca cggaacgcgt
     2221 tgcaatgatg cccagtgcgt aagggatctg aatcgcaaat ttgttcgtct cctcttcctg
     2281 atcaggaatg ccgaacagag taaaggcagc aggcgcaggt tgcgtttccc actcggcttc
     2341 aatagcagcc agtttggttt tctgcacgtc gcccatttcg tagccggatt catcacccag
     2401 aacaataaca gacagaacag cagccatacc gaagctggca gcgatagcaa aggagcgttt
     2461 agcgaaggcg aagtcacgac ctttcagcat ataccatgcg ctgataccga ggatgaacat
     2521 cgcgccagtc acataaccag acgctacagt gtgaacgaat ttcacctgag caaccgggtt
     2581 aagcaccagc tcggagaagc tcaccatctc catacgcata gtttcaaagt tgaaatcgga
     2641 cgcgattggg ttttgcatcc agccgttcgc aaccagaatc cacagtgcgg acaggtttga
     2701 accgagcgcc accagccagg tgacacacat atgctgaact ttacccagac gatcccaacc
     2761 gaagaagaac agacctacaa aggtggattc gaggaagaag gccatcagac cttcgattgc
     2821 cagcggcgca ccgaagatat cccctacata gtgggaatag taagaccagt tagtcccgaa
     2881 ctggaactcc atggtcagac cggtagccac acccagagcg aagttgatac caaacaactt
     2941 gccccagaac ttggtcatat ctttataaat ctgtttgccg gagaggacgt agaccgtttc
     3001 cataatggcc agcaggaacg ccataccgag cgtcagtggc acaaaaagga agtggtacat
     3061 cgcggtcaag gcaaactgta agcgcgacag ttcgactata tctaacatca tgactccttg
     3121 ctcatcgcat gaagactccg agagtgaacc ccgttagaaa gggtcacacg catgccccaa
     3181 tagaaatttg tttgaacact ttcatcatac catgtgaatg cacacaaaaa cggcgtaaag
     3241 aaaggttgca aacatgttaa taaaaactca aattgatccc acgtatatat tacgccgcaa
     3301 aatccttaca ataaacaggt ttttgttgaa gcaaatttgc atttttcgac
//
LOCUS       DAZFQD010000250         3269 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.252, whole genome shotgun sequence.
ACCESSION   DAZFQD010000250 DAZFQD010000000
VERSION     DAZFQD010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3269
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.252"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            535..1481
                     /gene="ompP"
                     /locus_tag="ACHKTW_005081"
                     /pseudo
     CDS             535..1481
                     /gene="ompP"
                     /locus_tag="ACHKTW_005081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193612.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease OmpP"
     gene            1574..3073
                     /locus_tag="ACHKTW_005082"
     CDS             1574..3073
                     /locus_tag="ACHKTW_005082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010892530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO6465940.1"
                     /translation="MRVIMKPLRRTLVLFIFSVFLCGTVSARQIEWQSCMTSPYSDWF
                     GTESPSPELLCGYLSVPLKYTDTGGDMSDENIPLVRLAMTKLPAKSKRKGSIIIISGG
                     PGLPGINPYINFDWPVTNLRESWDIIGFDPRGVGQSFPAINCQQPNQERSVNVSEKQL
                     ILQKINACIHNTGAEVIRHIGSHEAVYDIERIRQALGDKQLTAVAYSYGTQIAALYAE
                     RFPSSIRSIVFDGVVDIDDLNDNFSWKLRQAHSYQETFDRFAAWCARTKSCPLSSDRN
                     QAIHQFHQLLLKLHNSPLTDSRGESISSDDLISLTTELLLWRSSWPTLATAVRQFSQG
                     IVSNEIETALNSSIASEKVSDALGVILCVDQSDEQLSQEQRKSRKKALADAFPAVNFE
                     REQSDLPEFCELWPIHRDLQQTRLKNTVLPSGLLFVAHKYDPTTPWINARKMADKFSA
                     PLLTINGDGHTLALAGTNLCVDEAVVRHLLFPGKSEDITCQGSGTGDTN"
BASE COUNT         1017 a          600 c          718 g          934 t
ORIGIN      
        1 tcattaagat aaatattatt tttctggtct actcgggaaa gtcgcgatat ccttatgaag
       61 gatggatgat gttttttata ttgagtttta atatgcctgg catattaaac cagagataag
      121 tatataatat ttccattcac agcgtcagat gacaccacat tttactccat tttcatagcg
      181 ggttatgatg gctctcggta gctaaatgat tttctggcgg aaatacagga agatagcata
      241 caatatcatt ttcattctat taactcctta aaataattat ttagtcaata ttgagaatca
      301 gtgattgctg aaaataaaca gataaggcta agctgttttt agataatatg aggacaatga
      361 atgttacaat tgcactataa ctaacagcgc attcatatct ttttgatata cctcacaaaa
      421 acaaacaatt aggttaaaca aaagctaaac aaattaagta ctgataattg acttaccggt
      481 gtacatcaaa agaatcatat acatatgtag aagtgacaga agggggtcat tgttatgcaa
      541 actaagcttc tggcaattat gcttgcagca cctgtggtat tcagttctca ggaagcttct
      601 gcttctgatt tcttcggccc ggagaaaata agtacagaga ttaatttagg tacactgagt
      661 ggcaaaacaa aagaacgggt ttatgagcct gaagaaggtg gacgtaaagt cagccaactg
      721 gactggaaat acagtaacgc cgccattctt aaaggcgccg ttaactggga gctgaatcca
      781 tggttatctg ttggtgcagc tggctggacc actctcaata gtcggggggg aatatggttg
      841 atcaggactg gatggattcc gggactcccg gaacatggac agatgaaagc aggcatcctg
      901 atacacgtct taattatgcc aacgaatttg atttgaacgt taaaggctgg tttttaaaag
      961 aatctgatta tcgccttgct attatggcag gttatcagga aagccgttac agttttaatg
     1021 ctacaggagg aacttatatt tacagtgaga atggtggttt ccggaatgaa acgggagcgt
     1081 tacctgataa aataaaagtg attggttata aacaacattt taaaattcct tatgtcggtc
     1141 tgacaggaaa ctaccgttac gataattttg agtttggtgg tgcatttaaa tacagcgggt
     1201 gggtcagggg atctgataat gatgagcatt atgtaagaca aactacattc cgaagcaaag
     1261 taaaaaacca gaattactat tctgttgcag ttaatgccgg ttactatatc accccagagg
     1321 caaaagtgta catcgagggt gtatggagtc gtctcacaaa taaaaaaggg gatacatctc
     1381 tttacgaccg tagtgataat acttcagagc ataataataa cggggctgga attgaaaatt
     1441 acaacttcat tacgacggcc ggtctgaagt acacgtttta acaactttaa ctgtcatcat
     1501 ctccggtgag aattataccg ccggagattc tgtctgttgg tcagcgtaat aatttaccaa
     1561 tgcaggagat gcaatgcgtg tgataatgaa gcctttaaga aggactttag tattatttat
     1621 cttttctgtt tttctttgcg gaactgtgtc tgccagacag atcgagtggc aatcctgtat
     1681 gaccagccca tacagtgact ggtttggtac agaaagccct tcacctgagt tgttatgcgg
     1741 atatctatct gtaccgctaa aatataccga cacaggtggg gatatgtcag atgaaaatat
     1801 accacttgtc agactggcga tgacaaagtt gccggcaaaa agtaaacgta aagggagtat
     1861 tattataata agtggcggac ctggattacc cggaattaat ccttatatca attttgactg
     1921 gccggtcaca aatcttcggg aatcatggga cattatcggt tttgatccac gaggtgtcgg
     1981 tcagtccttt cctgccataa actgccagca acccaatcaa gaaagatcgg tgaatgtaag
     2041 cgaaaagcaa ctaattttac agaaaattaa tgcctgtatc cataatacag gagctgaagt
     2101 cattcgccat attggatctc atgaggctgt atatgatatt gaacgcatca gacaagctct
     2161 gggagataaa caactgacag ctgtagcata ttcatacggt acacagattg cagccttata
     2221 tgccgaacgc tttccatcca gtatcagatc tatagttttt gacggtgtcg ttgatattga
     2281 tgatttaaat gataattttt catggaaact caggcaggcc cacagttatc aggaaacgtt
     2341 tgatcgtttt gctgcatggt gtgccaggac aaaaagttgt ccactttctt cagacaggaa
     2401 tcaggcaata catcagttcc atcagctttt attaaaatta cataattctc ctttaacaga
     2461 cagcaggggg gaaagtatat cttcagatga tctcatatca ttaacgacag agcttctgtt
     2521 atggcgctca tcatggccaa cacttgcaac ggccgtacgt cagttctctc agggtattgt
     2581 cagcaatgaa attgaaactg cacttaattc ttccatagca tcggaaaaag tcagtgatgc
     2641 tctgggtgta atattatgtg ttgatcagag tgatgaacaa ttatcacagg aacaacgaaa
     2701 atcgcgaaaa aaagcccttg ctgacgcatt tccggccgtt aattttgaga gggaacagtc
     2761 cgatttacct gaattttgtg aattatggcc aattcatcgc gatctgcaac agacacgcct
     2821 gaagaatact gttttaccat ccggtttact gtttgttgca cacaaatatg atccgacaac
     2881 tccctggata aatgcccgta aaatggcaga taaattttca gctccgttac tgactattaa
     2941 cggtgacggg catacgctgg cgcttgctgg cactaattta tgtgtggatg aggctgttgt
     3001 tcgtcacctc ctgtttccgg ggaaatctga agatataacg tgtcagggaa gtggtaccgg
     3061 agacactaac tgatattaaa taaaatatta ctcccgacat taaataacat cagtgctttg
     3121 atgtcagggc ctgctaaccc tcttttgata tcagttgagc gcaggaaacg ccagttaaga
     3181 ggggacagga cttaggataa ataagagagt ggcaatcatg agtaagattc atgtacatgc
     3241 aggttacgga aagaggatat caggcaatg
//
LOCUS       DAZFQD010000251         3246 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.253, whole genome shotgun sequence.
ACCESSION   DAZFQD010000251 DAZFQD010000000
VERSION     DAZFQD010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3246
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.253"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            110..1630
                     /locus_tag="ACHKTW_005083"
     CDS             110..1630
                     /locus_tag="ACHKTW_005083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HFO6465941.1"
                     /translation="MSLSIVHTRAALGVNAPPITVEVHISKGLPGLTMVGLPETTVKE
                     ARDRVRSAIINSGYEYPAKKITINLAPADLPKEGGRYDLPIAIALLAASEQLTANKLD
                     EYELVGELALTGALRGVPGAISSATEAIKSGRKIIVAKDNEDEVGLINGEGCLIADHL
                     QAVCAFLEGKHALERPKPTDAVSRALQHDLSDVVGQEQGKRGLEITAAGGHNLLLIGP
                     PGTGKTMLASRINGLLPDLSNEEALESAAILSLVNAESVQKQWRQRPFRSPHHSASLT
                     AMVVGGAIPGPGEISLAHNGVLFLDELPEFERRTLDALREPIESGQIHLSRTRAKITY
                     PARFQLVAAMNPSPTGHYQGNHNRCTPEQTLRYLNRLSGPFLDRFDLSLEIPLPPPGI
                     LSKTVVPGESSATVKQRVMAARERQFKRQNKLNAWLDSPEIRQFCKLESEDAMWLEGT
                     LIHLGLSIRAWQRLLKVARTIADIDQSDIITRQHLQEAVSYRAIDRLLIHLQKLLT"
     gene            complement(1655..1993)
                     /gene="maoP"
                     /locus_tag="ACHKTW_005084"
     CDS             complement(1655..1993)
                     /gene="maoP"
                     /locus_tag="ACHKTW_005084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HFO6465942.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            2112..2951
                     /gene="hdfR"
                     /locus_tag="ACHKTW_005085"
     CDS             2112..2951
                     /gene="hdfR"
                     /locus_tag="ACHKTW_005085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HFO6465943.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNTLIRDLLKINVLD
                     EVY"
     gene            complement(3047..3122)
                     /locus_tag="ACHKTW_005086"
     tRNA            complement(3047..3122)
                     /locus_tag="ACHKTW_005086"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3087..3089),aa:Trp,seq:cca)
     gene            complement(3131..3207)
                     /locus_tag="ACHKTW_005087"
     tRNA            complement(3131..3207)
                     /locus_tag="ACHKTW_005087"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3171..3173),aa:Asp,seq:gtc)
BASE COUNT          873 a          795 c          801 g          777 t
ORIGIN      
        1 aactcaactt cacttgcaca aataaacagg aatcagccac taaaattcac gaattagcgg
       61 gctacagcag cgatatctta tgcacataat ggtgactcaa ggagggctta tgtcactgtc
      121 aattgttcat acccgcgcag ccctgggagt aaatgcgccc ccgatcactg ttgaggtaca
      181 tatcagtaaa ggtctacccg gcttaacgat ggtgggctta ccagaaacaa cggtaaaaga
      241 agctcgcgat cgcgtgcgca gcgccattat caatagcgga tatgaatatc cggcgaaaaa
      301 aatcaccatc aacctggctc cagccgatct gccaaaagaa gggggacgat atgatttacc
      361 tatcgccatt gcgttgctgg cggcctcaga acagcttaca gccaataagt tagatgaata
      421 tgaattagtc ggagaactgg ctcttacagg cgctctgcgt ggcgttcccg gtgcaatctc
      481 cagtgcaact gaagctatta agtcgggcag aaaaattatc gtcgcgaaag ataacgaaga
      541 tgaagtgggg ctaattaacg gtgaaggatg cctgatagcc gatcatctgc aagctgtctg
      601 tgcgtttctg gaaggtaagc acgctctcga acgcccgaaa ccaactgatg cagtatcccg
      661 ggcgctacaa catgatctca gtgatgttgt cggtcaggaa caaggaaagc gaggactgga
      721 aattaccgcc gctggcgggc acaacctttt actgattggg ccgccgggaa caggtaaaac
      781 aatgctcgcc agccgtatta atggcctgtt gccagattta agcaatgaag aggcactgga
      841 gagcgctgcg atattaagtc tggtaaatgc tgaatcagta caaaaacaat ggcggcagcg
      901 cccgttccgc tcacctcatc acagtgcgtc gttaactgcg atggtggtcg gtggtgcaat
      961 tccagggcca ggtgaaattt cgttggcgca taacggcgtg ctttttcttg atgagctacc
     1021 tgaatttgaa cggcgtacac tggatgcctt gcgagagccg attgaatccg ggcagatcca
     1081 tctttcacgc acacgagcaa aaataaccta tccagcccgt ttccagcttg ttgcggcgat
     1141 gaatcccagc cctaccggac attatcaggg aaaccataac cgctgcacgc cagaacagac
     1201 attacgttat ctcaaccggc tctcggggcc ctttctcgac cgcttcgatc tctcactgga
     1261 gatcccatta ccaccccccg gcattttgag taaaacggta gtgccgggag aaagcagcgc
     1321 caccgttaaa caacgcgtaa tggccgccag agagcgccaa tttaagcggc agaataaact
     1381 gaatgcctgg ctggatagtc cggaaatacg ccaattctgc aaacttgaga gcgaagatgc
     1441 gatgtggttg gaaggaacac tgatccatct ggggttatcg attcgtgcct ggcagcggtt
     1501 attgaaagtt gcacgaacca ttgctgatat tgatcagtct gacattatca cacgtcagca
     1561 tttgcaggag gcagttagct atcgtgcgat tgaccgtttg ctcatccatc tgcaaaaact
     1621 actgacataa aaaaagggca tttcgccctt tttattaatc atcagaatcg gtgtagtctt
     1681 cagcaccttc aacctgcggt ttaccgccgg aaagggtgtg aaaacgtttt ggacgcttaa
     1741 tacgcgtcat atacttggac cacacgcgtt ctgcttctgt cactggctca cgttcgccac
     1801 ggcatactgc tacgaagagt ttctcttcct cggtaaccgg ctcgcgtttg ccaagatcca
     1861 actcattgaa ggcataacca tgacgctcaa gcagttgtgc ctctttgatg gtgaaatcac
     1921 catgacgaga gaatccacgt ggataatgtt tattgtcgaa atatcgatta gtcgtcgtaa
     1981 agctttccgc catcctgcac gctcctaatt ctttgaccga gctagttatg gcgcggagta
     2041 ttagttacgc ttgacagagt gtaaaacaaa acatttaaat cataacgaca aataattttg
     2101 cggagagcac tgtggatacg gaattgttaa aaactttcct ggaagttagc cgaacgcgtc
     2161 actttggtcg agcggctgaa tcgctctatc tgacccagtc agcagtgagc tttcgaatca
     2221 gacaactgga aaatcaactg ggtgtgaacc ttttcacccg ccacagaaac aatatccgtt
     2281 taaccgctgc cggtgaaaaa ctactgcctt atgcagaaac gctcatgagc acctggcagg
     2341 ccgcccgtaa ggaggtggcg catacctcac gacataacga gttttctatc ggtgccagcg
     2401 cctcgttgtg ggaatgtatg cttaatcagt ggctgggacg cttgtatcaa aatcaggatg
     2461 cccatacagg cttacagttc gaagcgcgaa ttgcccaacg gcagtctctg gtaaaacagc
     2521 tgcatgaacg ccagcttgat cttcttatta ccactgaagc gcccaaaatg gacgaattta
     2581 gtagtcagtt gctgggatat ttcactttag cgctttatac cagtgcccct tcaaaactaa
     2641 agggagatct taattatctg cgacttgagt gggggccaga ttttcaacag catgaggcag
     2701 gtttgatcgg tgctgacgaa gtgcccattc tgacaaccag ttctgctgaa ctggcacagc
     2761 aacagattgc gatgcttaat ggttgcacct ggctacccgt cagctgggcg cgtaaaaaag
     2821 gcggcctgca taccgttgtc gatagcacaa cactttcacg gccgctttat gccatatggc
     2881 tgcaaaatag cgataaaaat acgttgattc gcgatctttt gaaaattaac gtgctggatg
     2941 aagtgtatta atatgaatgg ctggcaagga tgccggtaga aggatttact tcggagaggg
     3001 ttatttcaga taaaaaaaat ccttagcttt cgctaaggat gatttctggc aggggcggag
     3061 agactcgaac tcccaacacc cggttttgga gaccggtgct ctaccaattg aactacgccc
     3121 ctaattaggg tggcggaacg gacgggactc gaacccgcga ccccctgcgt gacaggcagg
     3181 tattctaacc gactgaacta ccgctccacc gaattctttt acaaccaccg gttttatgac
     3241 cggctt
//
LOCUS       DAZFQD010000252         3234 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.254, whole genome shotgun sequence.
ACCESSION   DAZFQD010000252 DAZFQD010000000
VERSION     DAZFQD010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3234
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.254"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..3173
                     /locus_tag="ACHKTW_005088"
     CDS             <1..3173
                     /locus_tag="ACHKTW_005088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO6465944.1"
                     /translation="ASRMGSEYYRTLQNGGRQAAAAAREQRRALAELNSQLTEIRASA
                     VGMTGAFAGAFATGHLISLADEWSSVNARLKQASQSSDEFASSQKVLMDISQRTGTAF
                     SDNAALFARSAASMREYGYSADDVLKVTEAISTGLKISGASAAEAGSVITQFSQALAQ
                     GVLRGEEFNSVNESGDRIIRALAAGMGVARKDLKAMADDGQLTADKVVPALISQLGIL
                     RDEYAAMPETVSGSITKVENAFMAWVGGANEASGATKTLSGALNGVAGNIDTVATAAG
                     VLVAVGVARYFGNLASGAMSATAGLVTAARNEVALAEAQLRGTQIATARARAAVYRAQ
                     QAVAAARGTEMQIAAEARLAVTQERLNRNIAARTAAQNALNSTTAVGSRLMSGALGLV
                     GGIPGLVMLGAAAWYTLYQNQEQAMESARQYALTIDEIAHKTPSMSLPEASDNEGRTR
                     EALTEQNRLIDEQASRVKSLQEKIAGYQYVLANPGWTTDNGFMINHLTSVKTVTEGLA
                     QATEQLAVEQSRLAQMQEKAQSIQDVLAGLEERRVVLIRQQAAEQNKAYQSLLVMNGQ
                     HTEFNRLLGLGNELLQQRQGLASVPLRLPQATLDDKQQSSLNNTERQLALSRLKGEEK
                     ERARLGYAADDLGLVGDTYQEARQRYIRNSMEAWRNNEANKPKSRGGKSETEKAEDSF
                     SRLLKQQKEQLALAGKNTELAKLKYQTSQGELKILTEIQKQELLRNAALIDQKKIREQ
                     LRAREETLKNDNADARASNDAELLGYGQGERIRERMRELKQIRDGYRQKDADLQSQYQ
                     TGDISEDFYRQALAQNAQYLSERLKDQEAFYAESDAQRADWQKGLQEGLSNWVDSASD
                     YASQAAQLATDGISGMVNNITEMLNGNKVEWRSWAALILQEISKVLMNAAIVNGIKMA
                     ANSMSGAGGFFGSIGNWLGGAVANAKGGVYTSANLSAYSNSIVDTPTYFAFAKGAGLM
                     GEAGPEAIMPLTRAADGSLGVRAVGSMNGSAGLVYSPVYHIAIQNDGANGQIGPEAAG
                     TLVQLIDQRVQAVMLSMRRDGGMLSG"
BASE COUNT          780 a          767 c         1083 g          604 t
ORIGIN      
        1 gggcgtcccg tatgggcagt gaatattacc ggaccctgca gaatggcgga cgtcaggctg
       61 ctgcggcagc cagggagcag cgccgggctc tggctgagct gaacagccag ttgacggaaa
      121 tccgcgcttc agctgtcgga atgaccggtg cgtttgccgg tgcctttgcc accggacacc
      181 tgatttcgct ggcggatgaa tggagctccg tgaatgcccg tctgaaacag gcgtcgcagt
      241 catcggatga atttgcgtca tcacagaaag tgctgatgga catcagccag cggacaggca
      301 ccgcattttc ggataatgcg gctctgtttg cccgctctgc cgcctcgatg cgtgaatatg
      361 gttacagtgc tgatgatgta ctgaaggtga cggaggccat ttcgacaggg ctgaaaattt
      421 ccggtgccag tgcggcagag gcgggttcgg tgatcaccca gttcagccag gcgctggcac
      481 agggggtgtt gcgcggcgag gaatttaatt ctgtcaatga aagtggggac cggatcattc
      541 gcgcactggc tgcaggcatg ggcgtggccc gtaaggatct gaaggcgatg gcggacgatg
      601 gtcaactgac ggcggataaa gtcgttcccg cgttaatcag ccagctgggg atattgcgtg
      661 atgaatatgc agccatgccg gaaacggttt ccggtagtat cacgaaggtg gaaaacgcct
      721 ttatggcctg ggtgggcggc gcgaatgagg ccagcggagc gacgaaaacg ctctccggcg
      781 cgctgaacgg tgtggccgga aatattgata ccgtggcaac agctgcgggt gtgctggttg
      841 ctgtcggggt ggcccggtac tttggtaatc tggcttccgg agcgatgtct gccacggcag
      901 gacttgtgac cgctgcacgt aatgaagtgg cactggctga ggcccagtta aggggaacgc
      961 agattgccac tgcgcgggca agggcagccg tgtaccgggc tcagcaggct gtggcggcag
     1021 cccgcgggac tgagatgcag attgcggcag aggcccgtct ggcggtcaca caggaacgcc
     1081 tgaacagaaa tattgctgcc agaaccgccg cccagaatgc gctgaacagt acaacggcgg
     1141 tgggctcacg tctgatgagc ggtgcgctgg gactggttgg cggtatcccc ggactggtca
     1201 tgctgggtgc agcagcatgg tacacgctgt accagaatca ggagcaggcc atggagtctg
     1261 cgcgccagta tgcactgacg attgatgaaa tcgcgcataa aacgccgtca atgtctttgc
     1321 ctgaagcctc agataatgaa ggacgaacac gggaggcgct gacagagcag aaccggctga
     1381 ttgatgagca ggccagccgg gtgaaatccc tgcaggaaaa aatcgccggg tatcagtatg
     1441 tgctggctaa tccgggctgg acgaccgata acggcttcat gataaaccat ctgacatcgg
     1501 tgaaaactgt aacggaaggg cttgctcagg caacagagca gcttgccgtt gagcagtccc
     1561 gtctggcaca gatgcaggaa aaagcgcagt ccattcagga tgtgcttgcc ggactggaag
     1621 agcgccgtgt tgtgttaatt cgtcagcagg cagcagagca gaataaagcg tatcagtcac
     1681 tgctggtcat gaacggccag catacggaat tcaaccgcct gctggggctg ggtaatgaac
     1741 tgctgcaaca gcgtcaggga ctggcgagtg taccgctgcg actgccacag gccactctgg
     1801 atgataaaca gcagagctcc ctgaataaca cagagcgtca actggccctg tcccggctga
     1861 aaggggaaga aaaagagcgt gcccggctgg ggtatgcggc ggatgacctt ggtctggtgg
     1921 gggatacgta tcaggaggcg aggcagcgtt acatccgtaa ttcgatggaa gcctggcgca
     1981 ataatgaggc gaataaaccc aaatcccggg gcggaaaatc tgagacggaa aaagcggaag
     2041 acagtttttc ccgtctgctg aagcagcaga aggaacaact ggcactggca gggaagaata
     2101 cagaactggc gaagctgaag taccagacct cgcagggcga gctgaaaatc ctgacggaga
     2161 tacagaagca ggagctgctg cgcaatgctg ccctgattga ccagaagaaa atccgggagc
     2221 agttacgggc ccgggaggag accctgaaaa atgataatgc agacgcaagg gcatcaaatg
     2281 acgcagagct gctgggatac gggcagggtg agcggatccg agaacggatg cgggagctga
     2341 agcagattcg tgacggctac cgccagaagg atgcggacct gcagtctcag tatcagacag
     2401 gggatatcag tgaggatttt tacagacagg cgctggcgca gaatgcgcag tatctgagtg
     2461 agcgccttaa agaccaggag gctttttatg ccgaatcgga tgcgcagcgt gctgactggc
     2521 agaaagggct gcaggaaggg ctaagtaact gggtggacag cgcatcagat tacgcttcac
     2581 aggcagcaca gcttgcgaca gacggtatct cagggatggt gaataacatc acggagatgc
     2641 tgaacggaaa taaagtggaa tggcgcagct gggctgcatt aatcctgcag gaaatatcaa
     2701 aagttcttat gaatgcggcc attgtcaacg gcattaagat ggcggcaaac agtatgtccg
     2761 gtgcaggagg atttttcggc agtataggca actggctggg tggcgcggtg gcaaatgcaa
     2821 aaggcggcgt ttatacctcg gcaaacctga gtgcatacag taacagtatt gtggacacgc
     2881 ccacgtactt tgcctttgca aaaggggcag ggctgatggg ggaggccgga cctgaagcca
     2941 taatgcctct gacccgggca gcggatggtt cactcggcgt gcgtgcggtg ggcagtatga
     3001 acggcagtgc gggtctggtg tattccccgg tgtaccacat cgccattcag aatgacggcg
     3061 ctaacgggca gatagggccg gaagcggcgg gcacccttgt gcaactgatt gaccagcggg
     3121 tacaggcggt gatgttatcc atgcgtcgtg acggaggaat gctgagtgga tgagattaag
     3181 acccttcact ggtgtccccg ggaagggatg caggtgacgg agaaaccgtc ggtg
//
LOCUS       DAZFQD010000253         3225 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.255, whole genome shotgun sequence.
ACCESSION   DAZFQD010000253 DAZFQD010000000
VERSION     DAZFQD010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3225
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.255"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(89..616)
                     /gene="mobB"
                     /locus_tag="ACHKTW_005089"
     CDS             complement(89..616)
                     /gene="mobB"
                     /locus_tag="ACHKTW_005089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418293.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobB"
                     /protein_id="HFO6465945.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKQLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTETPDEEELDLQFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQNG"
     gene            complement(598..1182)
                     /gene="mobA"
                     /locus_tag="ACHKTW_005090"
     CDS             complement(598..1182)
                     /gene="mobA"
                     /locus_tag="ACHKTW_005090"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HFO6465946.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLELNGKPLWQHVADALM
                     AQLSHVVVNANRHQEIYQASGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPEDLAARLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQVGERRVMAF
                     MRLAGGHAVDFSDNKDAFINVNTPEELARWQEKR"
     gene            1252..1521
                     /locus_tag="ACHKTW_005091"
     CDS             1252..1521
                     /locus_tag="ACHKTW_005091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HFO6465947.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDLNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            1598..2584
                     /gene="srkA"
                     /locus_tag="ACHKTW_005092"
     CDS             1598..2584
                     /gene="srkA"
                     /locus_tag="ACHKTW_005092"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HFO6465948.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEQGIRVDSGLTPLNSYENRVYQF
                     QDEDRRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAEQWMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRRWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            2601..>3225
                     /gene="dsbA"
                     /locus_tag="ACHKTW_005093"
     CDS             2601..>3225
                     /gene="dsbA"
                     /locus_tag="ACHKTW_005093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HFO6465949.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEKK"
BASE COUNT          813 a          777 c          787 g          848 t
ORIGIN      
        1 aggccatccg tcaggatggc ctttttgcat tggcgcagaa aaaaatgccg gatgcgacgc
       61 tggcgcgtct tatccagcct actcttgctt atccgttctg cttttgcatc cactccacca
      121 caaaatcagc cagcccctca acatcattaa tatccagtaa tgcgacatca agattaagcg
      181 gcacatcact ggctacagca ataacatgcc tgtctatcac taattcttca ggtcgatgtc
      241 cggctccatc gcgaaacagc acaatctttg cgatctcttc atgcttaaac ccttcgacca
      301 gaatcaaatc cagctttgag gtatccattc gacttgcgag aaactgtaga tccagctctt
      361 cttcgtctgg tgtttccgtc atcaaggccc atcgttgctg gctggcaacg atggtttgtg
      421 ccgcgccagc cttgcgcagc tcatagctat ctttgcctgg cttatcaaca tccatatcat
      481 ggtgcgtatg cttaatcagc cctggacgga tccctctggc gcataatgcc gggatcagtt
      541 gtttcaaaag cgtagttttt ccggtaccac tccacgcggc aaaggcgagt aacggtatca
      601 tcgtttttcc tgccatcggg caagctcctc tggcgtattc acgttaataa atgcatcttt
      661 attatcgctg aaatcaaccg catgaccgcc agccagacgc ataaatgcca ttacccggcg
      721 ttctcctact tgcagatatt ccagtaataa aggctcaatt gcgcggttta ccagagcaat
      781 agtcgggtga tcgcgttcac cgtcgtggac ccacacgaca ggcgcatctt tgcgctgatg
      841 attaagccgg gctgctaaat cctcgggaat ataaggagta tcgcacggac aaaacaaaaa
      901 ccactcgcct gcttcctgct gcattactga aagcattcct gccagagggc ctgggtaatc
      961 cgccagtgaa tcttcaatca ctttcagacc gcttgcctga tagatttcct gatgacgatt
     1021 agcattaacc acgacgtgag agagctgcgc cataagcgcg tcagcgacat gttgccataa
     1081 tggtttgccg tttaattcca gcaatccttt atctacgccg cccattcgtc tggctttacc
     1141 gcctgccagc acaacgcctg ttatcgtcgt catcagattc accgatatcg cctcttttat
     1201 tgtgggattg accctgctaa cgtgtctgtc tcaagagtaa ggagcattac tatgaaatgt
     1261 aaacgtctga atgaagttat tgaactcctc cagccagcct ggcaaaaaga gccagacctt
     1321 aacctgttgc aatttttgca gaaactggcg aaagagtcag gttttgacgg cgaactggcg
     1381 gatttgacgg atgacattct gatctatcac ctgaaaatgc gtgattccgc gaaagatgcg
     1441 gtgatcccgg gtttgcagaa agattatgaa gaagacttca aaacggcgct gttacgcgct
     1501 cgcggcgtaa ttaaagagta aaagcttgta agcggtgcca ccaaaatcat cgtgaaatga
     1561 tatccttcat cattcgtaat gttttccgga tgatgggatg aacaacagcg cttttacttt
     1621 ccagacacta cacccggata ccatcatgga tgctctattt gagcaaggga tccgggtgga
     1681 ttccggtctt accccgctta acagctatga aaaccgtgtc tatcaatttc aggacgaaga
     1741 tcgtcgacgt tttgtcgtca aattttatcg ccctgaacgt tggacagccg atcaaatcct
     1801 cgaagaacat caatttgcgt tgcagttggt aaatgatgaa gttccggtcg cagcacctgt
     1861 ggcctttaac ggtcagactt tattgaatca tcagggattt tatttcgctg tttttccaag
     1921 cgtcggtggt cgccagttcg aagctgataa tatcgatcag atggaagcgg ttgggcgtta
     1981 tttagggcgt atgcaccaga cggggcgcaa acagcttttt atccatcgcc cgaccatcgg
     2041 tctgaatgaa tatctcattg agcctcgcaa gctgtttgag gacgctacac tgataccttc
     2101 cgggttgaaa gcggcattcc tgaaagcgac agatgagctg atcgccgccg ttacagcaca
     2161 ctggcgggaa gacttcaccg ttctgcggct acatggagac tgccacgccg ggaatattct
     2221 ctggcgcgat ggtccaatgt ttgttgatct ggatgatgca cgtaatggtc cagctattca
     2281 ggatttgtgg atgttgctca atggcgataa agccgagcag tggatgcaac tggaaactat
     2341 tattgaagct tatgaagaat ttagcgagtt cgacaccgct gaaatcggac tgattgaacc
     2401 tttacgcgcc atgcgtttgg tttattatct tgcctggcta atgcggcgtt gggctgatcc
     2461 cgcgttcccg aaaaattttc cgtggttaac cggggaagat tactggctgc gacagacggc
     2521 gacttttata gaacaggcaa aagttctaca agaaccccct ttgcaattaa cacctatgta
     2581 ttaatcggag agagtagatc atgaaaaaga tttggctggc gctggctggt ttagttttag
     2641 cgtttagcgc atcggcggcg cagtatgaag atggtaaaca gtacactacc ctggaaaaac
     2701 cggtagctgg cgcgccgcaa gtgctggagt ttttctcttt cttctgcccg cactgctatc
     2761 agtttgaaga agttctgcat atttctgata atgtgaagaa aaaactgccg gaaggcgtga
     2821 agatgactaa ataccacgtc aacttcatgg gtggtgacct gggcaaagat ctgactcagg
     2881 catgggctgt ggcgatggcg ctgggcgtgg aagacaaagt gactgttccg ctgtttgaag
     2941 gcgtacagaa aacccagacc attcgttcag catctgatat ccgcgatgta tttatcaacg
     3001 caggtattaa aggtgaagag tacgacgcgg cgtggaacag cttcgtggtg aaatctctgg
     3061 tcgctcagca ggaaaaagct gcagctgacg tgcaattgcg tggtgttccg gcgatgtttg
     3121 ttaacggtaa atatcagctg aatccgcagg gcatggatac cagcaatatg gatgtttttg
     3181 ttcagcagta tgctgataca gtgaaatatc tgtccgagaa aaaat
//
LOCUS       DAZFQD010000254         3138 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.257, whole genome shotgun sequence.
ACCESSION   DAZFQD010000254 DAZFQD010000000
VERSION     DAZFQD010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3138
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.257"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..623)
                     /locus_tag="ACHKTW_005094"
     CDS             complement(<1..623)
                     /locus_tag="ACHKTW_005094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO6465950.1"
                     /translation="MGKGGGKGHTPVEAKDNLKSTQMMSVIDAIGEGPVEGPVKGLQS
                     ILVNKTPLTDTDGNPVIHGVTAVWRAGEQEQTPPEGFESSGAETVLGVEVTKAKPVTR
                     TITSANIDRLRVTFGVQSLVETTSKGDRNPSSVRLLIQLQRNGNWVTEKDVTINGKTT
                     SQFLASVILDNLPPRPFNIRMVRETADSTTDQLQNKTLWSSYTEIIDV"
     gene            complement(858..1439)
                     /locus_tag="ACHKTW_005095"
     CDS             complement(858..1439)
                     /locus_tag="ACHKTW_005095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310263.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFO6465951.1"
                     /translation="MNTAAEAIRALSLQVPGFRRQMNEGWYQIRIAGYDTAPEAVYAR
                     LHEQLGEGTVIHIVPRLAGAGKGGLQIVLGAAAIVGSFFTAGASMALWGTALSAGGFS
                     ATTMLFSLGASMILGGVAQMLAPKAKTPDYRATDNGRQNTYFSSLDNMIAQGNPMPVP
                     YGEMLVGSRRISQDISTRDEGGGGKVVVIGRQG"
     gene            complement(1436..2179)
                     /locus_tag="ACHKTW_005096"
     CDS             complement(1436..2179)
                     /locus_tag="ACHKTW_005096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFO6465952.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVISTPEGDRYQPCVNISAEPE
                     AYFRIAPEDWLRAQMQGEIVALVHSHPGGLPRLSEADRRLQIKSALPWWLVCRGDIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHREDEWWRNGQNLYLDNMEAS
                     GFYRVPLSSAQAGDILLCCFGASVPNHAAIYCGNGELLHHIPEQLSKRERYSEKWQRR
                     THSVWRHRHWHASAFTGIYNDLVAASACM"
     gene            complement(2190..2888)
                     /locus_tag="ACHKTW_005097"
     CDS             complement(2190..2888)
                     /locus_tag="ACHKTW_005097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO6465953.1"
                     /translation="MQDIHEESLNESVKSEQSPRVVLWEIDLTVQGGERYFFCNELNE
                     KGEPVTWQGREYQAYPIDGSGFEMNGKGSSARPSLTVSNLFGLVTGMAEDLQSLVGAT
                     VVRRRVYARFLDAVNFVAGNPEADPEQELSDRWVVEQLSELTAMKASFVLATPTETDG
                     ALFPGRIMLANTCMWTYRSDECGYTGGAVADEFDKPTTDIRKDRCSKCMRGCELRGMV
                     ANFGGFLSINKLSQ"
     gene            complement(2888..>3138)
                     /locus_tag="ACHKTW_005098"
     CDS             complement(2888..>3138)
                     /locus_tag="ACHKTW_005098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO6465954.1"
                     /translation="YQQRRPAGLNAQLKTFQVVFRVTTDAEREALSAFLSWHGGYRAF
                     LWKPPKHNRTVRVVCREWSITDNARYSDFSCTIEQVVN"
BASE COUNT          665 a         1019 c          772 g          682 t
ORIGIN      
        1 acatcgatga tttcggtgta tgacgaccag agcgtcttat tctgcagctg gtccgtggtg
       61 ctgtccgccg tctccctgac catccggatg ttaaaggggc ggggaggcag attatccaga
      121 atcaccgagg ccaggaactg tgaggtggtc ttgccgttaa tggtgacatc cttttctgtc
      181 acccagttac cgttacgctg taactgaatc agcagacgga cagaggacgg attacggtca
      241 ccctttgacg tggtctccac cagtgactgc accccgaagg tcacccgcag gcggtcaatg
      301 ttcgctgacg taatggtgcg tgtcaccggt tttgccttcg tcacttccac acccagtaca
      361 gtttcagctc cggaggactc aaagccttcc ggtggtgtct gctcctgctc cccggcgcgc
      421 cagaccgcgg tcacaccgtg tatcacggga ttaccgtccg tgtccgtcag cggggttttg
      481 ttcaccagga tactctgcag tcccttcacc gggccttcca ccggtccctc tccaatcgca
      541 tcaatcacgc tcatcatctg cgtggatttg agattatcct ttgcctcaac cggcgtgtgc
      601 cccttgccgc cacctttgcc cattgtctca ccctttactg tgataactgt tacgcacaaa
      661 aacaacaggc atcccggagg atgcctgtat catgactgaa taaaaattct gaatttcttc
      721 acattctgaa aaaaaagact gttgcgctga taaatacgct gcgttacttt ttagtgcctg
      781 aaaaataact ccataacgtt aatcttcatc gttctctccc gcagctccgc gatcactgcg
      841 ggattttttt attcttttta cccctgccgc ccgataacca cgacctttcc gcccccgcct
      901 tcatcacggg tgctgatgtc ctgggatata cggcgggagc caaccagcat ttccccgtaa
      961 ggcaccggca tcgggttccc ctgggcaatc atgttatcca gcgaggaaaa gtacgtgttc
     1021 tgtctgccgt tatccgttgc gcggtaatcc ggtgtttttg ccttcggggc cagcatctgg
     1081 gccacaccgc ccagaatcat gctggctccc agtgaaaaca gcatcgtggt ggcagaaaaa
     1141 ccgccggcac tcagggctgt accccataac gccatcgagg caccggcagt gaagaaagag
     1201 cccacgatgg ctgccgcccc cagcacaatc tgcagtccac cctttccggc cccggccagt
     1261 cgcggcacaa tgtggatgac cgttccctca cccagctgtt cgtgaagacg ggcgtacacc
     1321 gcctccggtg ccgtgtcata accggcaata cgtatctggt accagccttc gttcatctga
     1381 cggcggaatc cgggcacctg taacgacagc gcccggatgg cttccgctgc cgtgttcaca
     1441 tacaggctga ggcggcgacc aaatcgttgt aaatccccgt gaaggcagat gcgtgccagt
     1501 ggcggtgacg ccagacagaa tgcgttcgtc gttgccattt ttcagaatac ctctcccgtt
     1561 tactcagttg ttcaggtata tggtgaagca gctcaccgtt gccacagtaa atggcggcat
     1621 gattcggcac cgatgcgcca aagcagcaca gcaggatatc gcctgcctgt gccgaggaca
     1681 ggggcacccg gtaaaacccg gaggcctcca tattgtccag atacaggttc tggccattgc
     1741 gccaccactc atcctcgcgg tgaaaatccg gcatctcaat ccccgccaga tggtatgcat
     1801 ccctgaacag cgtgtaacag tccgtcaccc cgtgttcaaa acgacgcccg gtcaggtgcg
     1861 gcacacagcg gaatttatga atgtcccccc ggcagaccag ccaccagggc agggcacttt
     1921 ttatctgcag ccgccggtcg gcctcgctca gcctgggcag gccaccggga tggctgtgga
     1981 ccagtgccac aatctccccc tgcatctgtg cccgcagcca gtcttccggg gcaatacgaa
     2041 aatacgcctc cggctctgca gagatattca cacacggctg gtaccggtca ccctccggcg
     2101 tgcttatcac gaagccgcaa gactccgcag gcgcacaccg ccgggcatgc gccagaatcg
     2161 ctgattcagt ctgtgtcata aaatgggatt tactgcgaaa gtttattaat ggaaaggaaa
     2221 ccgccgaaat tagccaccat gccgcgcagc tcacacccgc gcatgcactt gctgcatctg
     2281 tccttacgga tatccgtggt gggtttatcg aactcatccg ccaccgcccc accggtgtaa
     2341 ccacactcat cagagcggta ggtccacata caggtgttcg ccagcatgat gcgaccggga
     2401 aacagcgccc cgtccgtctc ggtcggtgtc gccagcacaa acgaggcctt catggccgtc
     2461 agctctgata actgctccac cacccagcgg tcgctcagct cctgctccgg gtccgcttcc
     2521 ggattgcccg ccacaaaatt caccgcatcc agaaaacgcg catacacccg gcggcggacc
     2581 accgtggcac ccaccagact ctgcaggtcc tccgccatcc cggtgacaag accaaacaga
     2641 ttggacaccg tcagcgacgg gcgggcactg ctgccctttc cgttcatctc aaagccgctg
     2701 ccgtcaatcg ggtatgcctg atattcccgc ccctgccagg tcaccggctc ccctttttca
     2761 ttcagctcat tgcagaaaaa atagcgctca ccgccctgca ccgtcaggtc aatttcccag
     2821 agcaccaccc gcggtgactg ctctgattta accgactcgt tcagactttc ttcatgaata
     2881 tcctgcatca gttcaccacc tgctctatcg tgcaactgaa atcactgtac cgggcgttat
     2941 ccgtaatact ccactcccgg cacaccaccc tgaccgtccg gttatgtttc gggggcttcc
     3001 acaaaaaagc ccggtaacca ccatgccatg acagaaacgc ggacagtgcc tcccgctcag
     3061 catccgttgt cacccgaaaa accacctgaa aggtcttcag ttgcgcattc agtcctgccg
     3121 gacgacgctg ctgataac
//
LOCUS       DAZFQD010000255         3133 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.258, whole genome shotgun sequence.
ACCESSION   DAZFQD010000255 DAZFQD010000000
VERSION     DAZFQD010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3133
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.258"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..610
                     /locus_tag="ACHKTW_005099"
                     /pseudo
     CDS             1..610
                     /locus_tag="ACHKTW_005099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311739.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
     gene            complement(629..1131)
                     /locus_tag="ACHKTW_005100"
                     /pseudo
     CDS             complement(629..1131)
                     /locus_tag="ACHKTW_005100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019843029.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
     gene            complement(1214..1432)
                     /gene="ygeI"
                     /locus_tag="ACHKTW_005101"
     CDS             complement(1214..1432)
                     /gene="ygeI"
                     /locus_tag="ACHKTW_005101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgeI"
                     /protein_id="HFO6465955.1"
                     /translation="MTNPIGINNLSQSSNIANATGDEVVSLDKHINTSATDTDQIQAF
                     IVSTWMAPFQNDMYSEDNPISPYYKIEW"
     gene            complement(1600..2976)
                     /gene="ygeH"
                     /locus_tag="ACHKTW_005102"
     CDS             complement(1600..2976)
                     /gene="ygeH"
                     /locus_tag="ACHKTW_005102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HilA family transcriptional regulator YgeH"
                     /protein_id="HFO6465956.1"
                     /translation="MDLENKFSYHFLEGLTLTEDGILTQGNEQVYIPQKELGVLIVLL
                     ESAGHVVLKDMIIESVWKNIIVSDESLTRCIYSLRCIFEKIGYDRCIETIYRKGYRFS
                     GQVFKTKINEDNTSDYSIAIFPFTTSLNTLDPLILNQELVQIISNKKIDGLYTYPMAA
                     TNFCNDHISQNSFLSRFKPDYFVTGRINQNNAVNTLYIELIDAKNLFLIASNHLPVDE
                     LHNTSQFIIDNILQTVHKPERSVRLAKQDQGYKNHYLSDEMLAGKKELYDFTPESIYR
                     AMTIFDRLQNKSDIQTLKTECYCLLAECHMSLALHGKSELELAAQKALELLDYVSDIT
                     TVDGKILAIMGLITGLSGQAKVSHILFEQAKIHSTDIASLYYYRALVHFHNEKIEEAR
                     ICIDKSLQLEPRRRKAVVIKECVDMYVPNPLKNNIKLYYKETESESHRVIIDNILKLK
                     QLTRICMR"
BASE COUNT          955 a          510 c          556 g         1112 t
ORIGIN      
        1 ttcagatcag cagccgttta tgattgatgg gataattgga tttctcggac attatcctga
       61 tttatataag gttgttgggg gctataaaga tctgaagaaa gctatagcgg agtgtaacaa
      121 atctacagcc taaatattta ttttaggaga atttgcaggt ggaatgatgg ggtccgaact
      181 cgtaaaatgg gttaaatcgc ataagataga tgctcatatt attacatttg tagcaaagat
      241 gccgtatatt gattcaataa aattgcttga agcaggtgcg aaaggatgcg tatggaaaac
      301 cagtcacccg gcgaaactaa atcgtgctat tgattcgatt agtaatggtt acacttattt
      361 tgatagtgta catatggatt gtgaaaaaat atcttccagg tattcctctg ataatcaact
      421 tacaaatcgt gagtcagaaa tattacaact catagctgat ggtaaaacaa acaaagaaat
      481 cgctaatttc ttgcagttaa gcagaaaaac agtcgaaact catagactca atatcatgaa
      541 gaaattagat gttcacagtg gtatcgagtt gatcaaaaca gctttacgta tgggtgtttg
      601 cactatataa aaaaaagagg ctttaggctc attttgctga agccttacga ttgtctagtt
      661 cattaagcct ggtatataca atatatattt tggggcatat tgctgtctct ttttttggtt
      721 atccattgca aaacccgcat tataagtccc caggcattgc cacgttactc cacaacgtga
      781 aaaatggtta tataaaatct cggttcctat tttaatgttc agacaagggt tatctaacaa
      841 atctcgttca tcttttatta ttccacgttt ttttaacagg ggaatatgca tctggtttat
      901 ctgcattaac caataatcta aactctttat attgttattc ttatctctgt tcttgcctat
      961 gctatccttt tttagattgc tttcaacaag agcgatagct ttaattaaat ttggttcgat
     1021 tctgaacatt gttcctgctt cattagagca agagttgctg gcgaaagaca tttttgtaat
     1081 aaatatacaa aacgtgaaga tggttaagaa tttaactttt ggtgagcgca tattttttca
     1141 tgtgtgagga ttgaatgaaa gggacgagat actattataa aagtatctcg ttatgattta
     1201 tttggtcaat cgtctaccat tcaattttat agtaaggtga gataggatta tcttctgaat
     1261 acatatcatt ttgaaaaggc gccatccatg ttgaaactat aaatgcttgt atttgatctg
     1321 tatcagtagc actggtgttg atgtgtttat ctaatgatac gacttcatca cccgttgcat
     1381 tcgctatatt tgaactttga gataaattat tgataccgat tggattagtc ataaagctcc
     1441 cttttttaat tgtccaggcc aatggtcttt acatcatact tttaactcta gagcatttta
     1501 aatatcttgc aataaggtga atatcttaga tatttttatc tctgttgttt aaataacaag
     1561 ttgcatacat ctaaataata aaattgcgtc aataaaaatt tatcgcatac aaattctcgt
     1621 cagctgcttt aatttcaaaa tgttgtcaat tataactcga tggctttcac tctcagtttc
     1681 tttatagtag agtttaatgt tgtttttgag cgggttaggc acatacatat ctacacattc
     1741 ttttatcaca actgcttttc gtcttctggg ttcgagttgt agtgatttgt ctatacaaat
     1801 ccttgcctct tcaatttttt cattatgaaa gtggactagt gccctatagt agtagagaga
     1861 ggctatatca gttgagtgta tcttagcctg ttcaaataag atatgagata cttttgcttg
     1921 tccagacaga ccagttatca gtcccataat agctaaaatt tttccatcga cagtggttat
     1981 gtctgataca taatctaaaa gctctaatgc tttttgagca gcaagttcaa gttcactttt
     2041 tccatgaagt gccaaagaca tatggcattc cgctagaagg caataacatt ctgtttttag
     2101 cgtctgtata tcacttttat tttgtaatct atcaaatata gtcatggccc tgtaaatgct
     2161 ttcaggggtg aagtcgtaaa gttctttctt tccggctaac atttcatctg ataaataatg
     2221 attcttatat ccttggtcct gcttagctaa tcttacagat cgttctggtt tatgtaccgt
     2281 ttgaaggata ttatctataa taaattgtga tgtattatgt agttcatcaa cagggagatg
     2341 attactggcg atgaggaaaa ggtttttagc gtcgatcaac tcaatgtata aagtgttcac
     2401 tgcattattc tggtttattc ttcctgtaac gaaataatct ggcttgaatc tgctcaagaa
     2461 tgaattttga gatatgtgat cattacaaaa atttgtcgca gccatcggat aggtatagag
     2521 accatcgatt tttttatttg aaatgatttg cactaattcc tgattaagta ttaatggatc
     2581 cagtgtattc aacgaagtag tgaaagggaa tatagctatg gaatagtctg aagtattatc
     2641 ttcattaatt ttagttttga aaacctgccc gctgaaacga taacctttcc ggtagattgt
     2701 ttctatgcaa cgatcatagc caattttttc aaaaatgcag cgcaaagaat agatacatct
     2761 tgtcagggac tcgtcactaa caataatatt tttccatact gattcgatga tcatatcttt
     2821 cagtacaaca tgaccagcgg attcaagtaa tactattaat acacctaact ctttctgtgg
     2881 aatataaact tgctcatttc cttgagtaag aattccatct tccgtgagcg ttaatccctc
     2941 aagaaaatga tatgagaatt tattttctaa gtccattagt taacctctat gaaaaaattg
     3001 gtttcttgca tcctgcttaa gcaatagaaa ttatggctat tcatatttat gtgtcattaa
     3061 cgctacagga aattacattc gacattctat ggttgtgttc actaatttat cttaggaatt
     3121 cagtgatgta atc
//
LOCUS       DAZFQD010000256         3051 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.259, whole genome shotgun sequence.
ACCESSION   DAZFQD010000256 DAZFQD010000000
VERSION     DAZFQD010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3051
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.259"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..434)
                     /locus_tag="ACHKTW_005103"
     CDS             complement(<1..434)
                     /locus_tag="ACHKTW_005103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6465957.1"
                     /translation="MQTGLSQRRYRAEWGKSTLEKLCNFVRFCAVLILRFFVAGGDDI
                     HLSESVGDCRVRTVKNYSYPYLTFVTLSVLGGIFLFIWWRNDVYRITYLYSFTTNCLV
                     SVSVVLSVFVSLCLLKKNLVDRSKFTGVTSYLKFFSGMCLLT"
     gene            complement(817..1860)
                     /locus_tag="ACHKTW_005104"
     CDS             complement(817..1860)
                     /locus_tag="ACHKTW_005104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO6465958.1"
                     /translation="MSKRKKYTGKRGTGSLADALKSAPSLSAFTFDGPYEASRLDLLD
                     NMSCADNGRYYDTPMDWNAIARASRRASWHQSALYFKRNALNGCFVPHPLLSRQAFSA
                     FALDWFVFGNAYLEVRRNKFGEPIALRPALAKYTRRGSDLDTYWYLNDDGSEFAFRKG
                     AVCHVLNPDINQEIYGMPEYIGGLLSVSLSNSADTFRKLYYDNGSHAGCIVYVGAAQA
                     NAESVEAIKKTLTESRGKGAFRNILLHAPGGGKDGVQILPFQQITAKDEFLNIKGSAR
                     DDILAAHRVPPQLMGAMPDGNAAFGDVEKAARVFFINELQPVMEAMKHVNEWLGVEVM
                     RFNPYSLLQDGAS"
     gene            complement(1866..>3051)
                     /locus_tag="ACHKTW_005105"
     CDS             complement(1866..>3051)
                     /locus_tag="ACHKTW_005105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="terminase family protein"
                     /protein_id="HFO6465959.1"
                     /translation="SRRQAYQFKHFIQQAALEVDVELKGGDKIVLSNGAELHFLGTSA
                     ATAQSYTGHLKFDEFFWVSNFVELRKVAGAMATLEGLTRTYFSTPSGETHEAYQFWTG
                     DRWNSEQPRSKRSEFDVSHAHLKGGVLFPDETWRQIVTIEDAVANGWRYTNIGVIRGE
                     NTESEFRNLYMCEFVRDGEAAFSLNTLVGCGVDGYDEWPDWKPFAVRPMGVREVWLGY
                     DANGSSGKGDCGALSVCVPPLVAGGRFRTVETVRVQGMEFEQQAALIEEYTQKYNVKH
                     IGIDVTGGNGEGVYQIVKKFFPLAVPYNFNIASKRALVMKMLQVVRAGRWEYDRSERD
                     LVQAFASVRKIQTPGGYITYDTDRSRGVSHGDLAWATMLAVINEPIGAEREDNRGFAM
                     EF"
BASE COUNT          801 a          847 c          746 g          657 t
ORIGIN      
        1 caggtaagaa ggcacatacc cgaaaaaaac ttaagatacg atgtaacccc ggtaaattta
       61 gaccgatcaa ccaggttctt tttcaacagg cacagtgaga caaaaaccga aagcaccaca
      121 gacactgaaa caaggcagtt tgtcgtgaac gaataaaggt aggtaattct gtaaacatcg
      181 tttcgccacc aaataaaaag aaaaatcccc cccaaaacag aaagcgtgac aaaagttaaa
      241 taaggatatg agtaattttt cacagttctc acccggcagt ctcccactga ttcagacaga
      301 tgaatatcat caccacctgc cacaaaaaat cgcaagatca aaactgcaca aaagcgcacg
      361 aaattgcaca atttttctaa tgtacttttt ccccattccg cccggtaacg gcgctggctg
      421 aggccggttt gcacggtgca cgaaaaacga ggcaattgca gcgcgcaggc gacgagggaa
      481 gggcgcccga attgaggggt ggaaggcagg ttgctttatt tggggtcgta ttgcgcgtga
      541 tacgccagca gtgaagcctg ttttaaaaaa ccacaccgaa acagagtcag aaaaacaggc
      601 cgccagaatg cgtctggttg agttttacgg ggtcgggatt aaatgcggaa acatcacacc
      661 gcaggcatga cgcgaaaaag agaaaaacca cagcaaaaca taatgttaaa tgttattgat
      721 gagttgagaa gcgaaaggtg tattgcgttg acagagctgg agagtcgggg cataaatttt
      781 ttatgccccg gcgaagcagc cgacaagcga agcgcgtcag ctggcgccat cctgaagcag
      841 cgaataggga ttaaagcgca tcacttcaac cccaagccat tcattgacat gcttcatcgc
      901 ttccatgacc ggttgcaact cgttgatgaa aaataccctc gccgccttct caacgtcacc
      961 gaacgccgcg ttcccgtcag gcattgcccc catcagctga ggcggtacac ggtgagccgc
     1021 aaggatatcg tcgcgggcac tccccttgat gttaaggaat tcgtccttcg cggtgatttg
     1081 ctggaacggc agtatttgca caccatcctt gccgccacct ggcgcgtgca gaaggatatt
     1141 gcggaacgcc cccttgccac gggattccgt gagtgttttc ttgatggctt ccacgctttc
     1201 agcgtttgcc tgggcggcac caacatagac aatgcacccg gcatgtgaac cgttgtcata
     1261 gtagagcttg cggaacgtat ccgctgagtt cgacagactc accgacagca acccaccgat
     1321 atactccggc atcccgtaga tttcctggtt gatatcgggg ttcagaacgt ggcacacagc
     1381 ccccttacgg aaagcaaatt cagaaccatc atcattgagg taccagtacg tatcgagatc
     1441 actgccccgc cgcgtgtatt tcgccagcgc aggacgaagc gcaatgggtt caccgaactt
     1501 gttgcggcgg acttcaagat aggcattacc gaacacgaac cagtccagcg caaacgcgga
     1561 aaacgcctgg cgggacagta gcggatgagg aacaaagcag ccgttcaggg cattacgctt
     1621 aaaatacagg gccgactggt gccatgatgc acgacgcgag gcgcgagcaa tggcattcca
     1681 gtccatgggg gtgtcatagt agcggccatt atcggcgcac gacatgttat caagcagatc
     1741 aagacggctt gcttcataag gaccgtcaaa cgtgaaagca ctgagtgacg gcgctgattt
     1801 cagtgcatca gccagtgagc ccgtgccgcg ctttccggtg tattttttac gtttactcat
     1861 tgtgttcaga actccattgc aaagccacga ttatcctcac gctctgcgcc gattggctca
     1921 ttgataacag caagcatggt tgcccaggcg agatcaccat gactgacacc gcgtgagcgg
     1981 tcagtgtcat aggtgatata gccgccgggc gtctgtattt ttcgcaccga tgcaaaggcc
     2041 tgtaccaggt cgcgctcgct gcggtcatac tcccagcgtc cggcacggac cacctgaagc
     2101 atcttcatga ccagcgcccg ctttgaagcg atattaaagt tatacggaac agccaggggg
     2161 aagaattttt tcactatctg ataaacccct tccccgttcc cgccggtgac atcaatcccg
     2221 atatgcttca cattgtattt ttgggtgtac tcctcaatca gggcggcctg ctgttcaaac
     2281 tccatgccct gaaccctgac ggtttccacc gtacgaaaac gcccaccggc aacaaggggt
     2341 ggcacgcaca ccgacagcgc cccgcagtcc cccttcccgc tgctgccgtt ggcgtcatac
     2401 ccgagccaga cttccctcac ccccataggg cggacagcaa aaggcttcca gtccggccat
     2461 tcgtcatagc catcaacacc gcacccgacg agcgtattca gactgaaggc tgcctcacca
     2521 tcacggacaa attcgcacat gtacagatta cgaaattcac tttctgtatt ttcgccgcga
     2581 ataacaccaa tattggtgta ccgccatccg ttagcgacag catcctcaat agtgacaatc
     2641 tgccgccatg tttcgtcagg aaagagcacg ccccccttca gatgcgcatg tgaaacatca
     2701 aactcactgc gttttgagcg tggctgttcg ctgttccagc ggtcccccgt ccagaactga
     2761 tacgcctcat gtgtttcacc tgacggtgtt gaaaagtagg ttcgcgtaag cccttccagt
     2821 gtcgccattg ccccagcaac cttcctgagt tccacaaagt tactgaccca gaaaaattca
     2881 tcaaatttaa ggtgtccggt atacgactga gcggttgctg ccgatgtgcc aaggaagtga
     2941 agctctgcac cgttagacaa aacaatcttg tccccccctt tcagttcgac atcaacttcc
     3001 agcgccgcct gctgaataaa gtgtttgaac tgatatgcct ggcgacggga t
//
LOCUS       DAZFQD010000257         3035 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.260, whole genome shotgun sequence.
ACCESSION   DAZFQD010000257 DAZFQD010000000
VERSION     DAZFQD010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3035
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.260"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(225..301)
                     /locus_tag="ACHKTW_005106"
     tRNA            complement(225..301)
                     /locus_tag="ACHKTW_005106"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(265..267),aa:Asp,seq:gtc)
     gene            complement(434..1165)
                     /gene="dnaQ"
                     /locus_tag="ACHKTW_005107"
     CDS             complement(434..1165)
                     /gene="dnaQ"
                     /locus_tag="ACHKTW_005107"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HFO6465960.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVEKKGGSCLWRA"
     gene            1230..1697
                     /gene="rnhA"
                     /locus_tag="ACHKTW_005108"
     CDS             1230..1697
                     /gene="rnhA"
                     /locus_tag="ACHKTW_005108"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HFO6465961.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHSWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            complement(1694..2416)
                     /locus_tag="ACHKTW_005109"
     CDS             complement(1694..2416)
                     /locus_tag="ACHKTW_005109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFO6465962.1"
                     /translation="MKPARVPQTVVAPDCWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDVCLLAHTLPW
                     CTDPHRLLREADRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQYRKPQA"
     gene            2450..>3035
                     /gene="gloB"
                     /locus_tag="ACHKTW_005110"
     CDS             2450..>3035
                     /gene="gloB"
                     /locus_tag="ACHKTW_005110"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HFO6465963.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAITANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVIATPGHTLGHICYFSNPYLFCGDTLFSGGCGRLFEGTASQMYQSLNKLSALPDDTL
                     VCCAHEYTLSNMKFALSILPHDLSINDYYRKVKEL"
BASE COUNT          829 a          724 c          705 g          777 t
ORIGIN      
        1 gaaatataca ttcatttaat caatataaat ttattctctg gaaatatatt taagctgaat
       61 ggttcgatat cattattgaa ttttaatgaa tgagacaaca agcgagaatc acgcacttac
      121 gccagaataa aatcattgaa cagagaaatg gtggaattca aatacaaaaa aaccgcaaaa
      181 ttaaaaatct tgcggctctc tgaactcatt ttcatgagtg aatttggcgg aacggacggg
      241 actcgaaccc gcgaccccct gcgtgacagg caggtattct aaccgactga actaccgctc
      301 cgcgttgtgt tccgttggga acgaggcgaa tattacggat tgcctcaccc ttcgtcaacg
      361 gtttttctca tcttttgaat cgtttgctgc aaaaatcgcc caagccgcta tttttagcgc
      421 cttttacagg tatttatgcc cgccagaggc agcttccgcc cttcttctcc accagatcaa
      481 gacgggcttc atgagctgca agctcttcat ctgtcgcaaa aacaacgcgt aacttacttg
      541 cctgacgtac aatgcgctga attgttgctt caccttgttg ctgctgtgtc tctccttcca
      601 tcgcaaaagc catcgacgtt tgaccaccgg tcatcgccag ataaacttcc gcaaggatct
      661 gtgcatcgag taatgccccg tgtagcgttc gtttactgtt atctatttcg tagcgggcac
      721 ataacgcatc gaggctgttg cgcttaccag gaaacatttt cctcgccacc gcaaggctat
      781 cggtgacctt acagaaggta ttggtcttcg gaatatcgcg cttaagcaac gaaaactcgt
      841 agtccataaa gccgatatcg aacgctgcgt tatggatcac caactccgcg ccgcggatat
      901 agtccatgaa ctcatcggct acttcggcaa acgtgggctt atcgagcaaa aattcatcgg
      961 caataccatg tacgccaaag gcttccggat ccaccagccg atcgggtttg agataaacat
     1021 ggaagttatt gcccgtcagg cgacggttca ccacttcaac ggcaccaatc tcaatgatct
     1081 tgtggccttc atagtgcgca ccaatctggt tcataccggt ggtttcggta tcgagaacga
     1141 tctggcgtgt aattgcagtg ctcatagcgg tcatttatgt cagacttatc gttttacagt
     1201 tcgattcaat tacaggaagt ctaccagaga tgcttaaaca ggtagaaatt ttcaccgatg
     1261 gttcgtgtct gggcaatcca ggacctgggg gttacggcgc tattttacgc tatcgcggac
     1321 gcgagaaaac ctttagcgca ggctacaccc gcaccaccaa caaccgtatg gagttgatgg
     1381 ccgctattgt cgcgctggag gcgttaaaag aacattgcga agtcattttg agtaccgaca
     1441 gccagtatgt ccgccagggg atcactcagt ggatccatag ctggaaaaaa cgtggctgga
     1501 aaaccgcaga caaaaaacca gtaaaaaatg tcgatctctg gcaacgtctt gatgctgcat
     1561 tggggcagca tcaaatcaaa tgggaatggg ttaaaggcca tgccggacac ccggaaaacg
     1621 aacgctgtga tgaactggcc cgtgctgcgg caatgaatcc cacactggaa gatacaggct
     1681 atcaagtcga agtttaagcc tgtggtttac gatattgccg ggtggctcca accgcctggc
     1741 gaattcgtgg cttgttttta ctctgtttca tcggatttag cgttaaagga atagtccgtt
     1801 tccgggcaac aataagttgt aagcaaccaa gcgcaggaat atgtgcattc aatagttttc
     1861 caccgtgttt gttccacggg agaacgtgga aacggctggc gtgcagcact tcaaaattca
     1921 acaaagagag ccagtccagt tgccgcatca gagtaaacat ccggctgtta tagggcgagg
     1981 ttttgcgcaa taccggcaca agtttgcgta atcccattaa actgatggga ttgaagccac
     2041 taatcaccag ccagccatca tcaatcaata cccgatcggc ttcacgcaat aaacgatgcg
     2101 gatcggtgca ccacggcaat gtatgtgcca gtagacaaac atcaacggat ttatcggcaa
     2161 aaggaagatg aagtgggtcc gcctgtacct ggacgggcat tccttgcgca gaaacattca
     2221 cttgatgaga aaccgcgcac gcttcgcaat tgatttctgc gcttaaattg ccaatcttaa
     2281 gcagatgaaa accatacatt ttagtgaacc acgggttgag ctggcgctcc agcgccttgc
     2341 gataaagctc tccccagggc aaatcgcccc agcaatcagg agccacgaca gtttgaggga
     2401 ctcttgccgg tttcatcaca accttccgtt tcacactaag aggtaatcta tgaatcttaa
     2461 cagtattccc gcctttgatg acaattacat ctgggttttg aatgatgaag caggtcgctg
     2521 cctgattgtc gatcccggag acgcagagcc agtattaaac gccattaccg ccaataactg
     2581 gcaaccggag gccatatttc tcacccacca tcatcacgat cacgttggcg gcgtaaaaga
     2641 actggtggaa aagtttccgc aaattgtggt gtatggtcca caagagacac aagataaggg
     2701 aacaacacag gtagtcaaag atggcgaaac tgccttcgtt ttggggcatg aatttagtgt
     2761 aattgccaca ccgggtcaca ctttaggaca tatctgttac ttcagtaacc cttatctatt
     2821 ttgcggcgac acgctgtttt ctggtgggtg tggtaggttg tttgaaggga cagcatcaca
     2881 aatgtatcaa tcacttaata agttaagtgc gttacctgac gatacattgg tatgttgtgc
     2941 tcatgaatat acgttatcaa atatgaagtt tgctttgagt attcttccgc acgatttgtc
     3001 cataaatgat tattatcgta aagttaagga gttac
//
LOCUS       DAZFQD010000258         2937 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.261, whole genome shotgun sequence.
ACCESSION   DAZFQD010000258 DAZFQD010000000
VERSION     DAZFQD010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2937
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.261"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..359
                     /locus_tag="ACHKTW_005111"
     CDS             <1..359
                     /locus_tag="ACHKTW_005111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFO6465964.1"
                     /translation="LRTLQSRIKTLNTRRVNILKGEQRRVSGSARVSLKRHIWLRDAG
                     QCCICGRVVDLCDSELDHRIALQFGGGNEETNLWTLCTECHRQKSAREAAGGMPDPTL
                     PEVSGGSGRADDIIGL"
     gene            649..1212
                     /locus_tag="ACHKTW_005112"
     CDS             649..1212
                     /locus_tag="ACHKTW_005112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFO6465965.1"
                     /translation="MLTTQKRKFALALMSGKNKTASAIAAGYSAKTARVKGSQLAKDP
                     EVLAFIARKQGETVEVDEVPVYRQKKSEQEDKPRRREAAAIPQQDETNPEMPPPVVIS
                     PSIDYMEDGLPDPVKAMGRILVENLIIDPKLALDAAWRLAQFTHYKKGDAGKKSAKGD
                     AAKKAANRFAVPPPPRLVVNNDNEGNG"
     gene            1209..2870
                     /locus_tag="ACHKTW_005113"
     CDS             1209..2870
                     /locus_tag="ACHKTW_005113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HFO6465966.1"
                     /translation="MIPVWSTACPDWAERLKKGLSIIPSPIYPEQAAHALAIFKQLRI
                     VDAPGSPTFGESCAQWVFDLVAALFGSYDAQTGVRHIKEVFILIPKKNSKSTLAAGIM
                     MTALLLNWRQAAGYTILAPTVEVAANAFNPARDMVRRDDDLDDLCQVQTHIRTITHRV
                     TDTTLKVVAADPNTVSGIKSVGTLIDELWLFGKQCKAEDMLREAIGGLASRPEGFVVY
                     TTTQSNEPPAGVFRQKLQYARDVRDGKIHDPHFLPVIFEHPPEMVESGAHLLMENLAM
                     VNPNLGYSVDEAFLYREYRKAREAGEEAFRGFMSKHANVEIGLALRSDRWAGADFWEQ
                     QGRRVSLEDILQRADVVTVGIDGGGLDDLLGMYVIGRDRETREWLGWGHAWAHETAVV
                     RRKSEASRFQDFVACGDMTIVRRVGDDTAEVAEYVRRIHEAELLDHIGIDPSGVGQIL
                     DSLAEAGIPDGIVVGISQGWKLGGAIKTTERKLAEGVLVHGGQPLMAWCVGNARVEPR
                     GNAILITKQASGRGKIDPLMALFNAVSLMSLNPEPKKKEYAVFFI"
BASE COUNT          673 a          712 c          926 g          626 t
ORIGIN      
        1 agcttcgtac actccagagc agaatcaaaa cactgaatac ccgacgggtg aatattctga
       61 agggggaaca gcgtcgtgtc agtggcagtg cacgtgtttc cctcaagcgt catatctggc
      121 tcagggacgc cgggcagtgc tgtatctgtg gtcgtgtggt tgacctctgt gacagtgaac
      181 ttgatcaccg aattgcactt cagttcggtg gtggtaatga ggagacgaat ctctggacgc
      241 tctgtaccga atgccatcga caaaagtctg ctcgtgaagc ggcgggtggt atgccggacc
      301 cgacgctgcc ggaggtgtcc ggaggtagtg gcagagcgga cgacatcatc ggactgtaac
      361 ccgacccggg ggggggatca tccggcgtaa aaaacgatcg ctccggacac cgcgccccct
      421 ctcatgcaga gaaaaaattc ccgtttcagg ccagttaaca tgttaactgg ctgttcggcc
      481 ttttttctgt ttttatcttt ataattcagt ttattgcgtg gaaaaaatgt taaccgactt
      541 tttcggaaaa tgttaaccag gcagcagtta acattcgggg tatgcgcggt gtgggaaaac
      601 ggcctgatgc atgcccggct gagagagttt tgaccccggg agggcactgt gctgacgacg
      661 caaaaacgaa aatttgcgct ggcgctcatg tccgggaaaa acaaaacagc gtcagccatt
      721 gcggcaggtt attcggcgaa gacggccaga gttaaaggct cgcagctggc aaaagatccg
      781 gaggtgcttg cgtttatagc ccgtaaacag ggcgagacgg tggaggtgga tgaggttcct
      841 gtttaccggc agaaaaaatc agagcaggag gataaacccc gtcgccgtga ggcggctgca
      901 ataccacagc aggacgaaac aaatccggag atgccaccgc ccgtggtgat atctcctagt
      961 attgattaca tggaagacgg tcttccagat ccggtaaaag caatggggag gatcctggtg
     1021 gaaaacctga taattgaccc gaaactggca ctggatgcgg cctggcgact ggcgcagttc
     1081 acacactata aaaaaggcga tgctggtaaa aaatcggcaa aaggtgatgc cgctaaaaaa
     1141 gcggctaacc gttttgcggt gccaccacca ccccgactgg tggtgaataa tgataatgag
     1201 ggcaacggat gatacctgtg tggagcacgg cctgcccgga ctgggcagag cgcctgaaaa
     1261 aggggctgtc gattattccg tctccgattt acccggagca ggccgcacat gccctggcga
     1321 tttttaaaca actgcggatt gtggatgcac cgggcagccc gacgttcggt gagtcctgtg
     1381 cacagtgggt gtttgacctg gtggcggctc tgtttggctc ctacgatgcg cagaccggtg
     1441 tccgccatat caaggaagtg ttcattctga tacccaaaaa aaacagcaag tccacactgg
     1501 ccgccgggat catgatgacg gcgctgttac tgaactggcg gcaggcggcg ggttacacga
     1561 ttctggcccc gactgtggag gtggcagcca acgccttcaa tcctgccagg gatatggtac
     1621 gacgggacga tgatctggat gacctctgcc aggtacagac ccatatccgg accatcaccc
     1681 acagggtgac agacaccacc ctgaaggtgg tggcagccga tccgaatacg gtgtccggta
     1741 tcaagtccgt gggtacgctg attgatgagt tgtggctgtt tggcaagcag tgcaaggcgg
     1801 aggacatgtt acgtgaagcc ataggcggcc ttgcctcccg cccggaaggg tttgtggtgt
     1861 atacgaccac ccagtcgaat gaaccgcccg ccggggtgtt cagacagaaa ctgcagtacg
     1921 cccgggatgt gcgcgacggc aaaattcatg atccgcactt tctgccggtg atatttgaac
     1981 accctcctga aatggtggaa agcggggctc acttgctgat ggaaaacctc gccatggtca
     2041 atccgaatct cggctattcg gtggatgagg cctttctgta ccgggagtac cgtaaagccc
     2101 gggaggctgg tgaggaagca tttcgtggct tcatgtcaaa acacgccaat gtggaaattg
     2161 gccttgccct gcgttctgac cgctgggcgg gcgcggattt ctgggagcag cagggcaggc
     2221 gcgtcagcct ggaagatatc ctgcagcgcg ctgatgtggt gacggtgggg attgacggcg
     2281 ggggcctgga tgatctgctg ggaatgtacg tgattggtcg tgatcgggag acccgcgaat
     2341 ggttaggctg gggccatgcc tgggcgcatg aaaccgcggt ggtccgacgg aagagtgagg
     2401 catcccggtt tcaggatttt gtggcctgtg gagacatgac gattgtccgt cgggtcgggg
     2461 atgacacggc ggaagtggcg gagtatgtgc gtcgtattca tgaggctgag ttactggatc
     2521 atatcggtat tgacccgtca ggtgtggggc agattctgga ttcactggcg gaagccggga
     2581 tccccgacgg aattgttgtg gggataagcc agggctggaa gctgggcggg gccatcaaaa
     2641 ccaccgaacg caaactggct gaaggggtgc tggtgcatgg tggccagcca ctgatggcct
     2701 ggtgcgttgg caatgcccgg gtggagccca gaggtaacgc catccttatt accaaacagg
     2761 ccagcggacg ggggaaaatc gacccgctga tggcgctttt caatgcggta tccctgatgt
     2821 ccctgaatcc ggagcccaaa aagaaagaat atgcggtttt tttcatataa ccctgttcac
     2881 cctgtaacca tcatggaccg ctgcggcggt ttttttattt tcaggaggct gatgtga
//
LOCUS       DAZFQD010000259         2919 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.262, whole genome shotgun sequence.
ACCESSION   DAZFQD010000259 DAZFQD010000000
VERSION     DAZFQD010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2919
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.262"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..384
                     /locus_tag="ACHKTW_005114"
     CDS             <1..384
                     /locus_tag="ACHKTW_005114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HFO6465967.1"
                     /translation="GLAEQLVNGYVDLLDGLPFGESREYRVTFRELTAKDSIDAESEA
                     ERLMETRNGPVLVASPALRGIALLRRQIAAVGQIEGPLSPRQIGQLSERDLSRLMAAV
                     SLLDSAMAGKLVAERGRPDAVPGSD"
     gene            512..2569
                     /locus_tag="ACHKTW_005115"
     CDS             512..2569
                     /locus_tag="ACHKTW_005115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFO6465968.1"
                     /translation="MAKNLKASLIVDLLGNISAKSRQWSQELGAFSRSGRTGLGGLGN
                     AARRAGQETELVGSRMQRTLAGVRGSIRTVTSDFDRLQGSITGTIGRISNLYGMLAGG
                     AAVYGFNKAFIRPAAEMENYILRLNAINHGDKAKTEAVKAWAVQNAKDTTWGLAGVMQ
                     EYASSRGFGMSDREARRFITMLQDQGGYHGWSLSDAQGASLQLKQMFARQSIQAADAN
                     ILTGYGINVYQLLADKLGVNQKLIREKGEKGKLGPDSIRLLFQVMAEQAKGAQKNAMN
                     SWTGMTSMMGDVWDQFAREVMAKGPFDSLKKSLKGFLDYADAAQKSGLQDKLATQTAS
                     ALNQGFEYARDAATGFYRAIQKVRETLQALRDAGYGDALDRIGQGAQTAAKYLMYMYL
                     ASRALKVLRFAGAGALRLGATPLRYGMAMTSVLTSPFRKPQTTVPGTQPGRAGRFLNF
                     LTGVNPAAVQPVLVTNWPAGGLASGGGDVVVSGDGKTVRDRKKRGPGRGRGVTTVVTA
                     GEQLAESAGKQGFFGRMMSRAGGLLSAAGNRMGLGRFAGLFRGAGRLGGGALWAGAMA
                     APVLLDSSASAADKAGAVGSLAGSIAGGALGAAAGPVGVAIGSTVGSYLGDYLGGWLT
                     QAWQKLRGSSDENGGQATAKTAARVELVAPEGWRARSIDVDDTAQHGLDVNVWNGGNY
                     GLY"
     gene            2556..>2919
                     /locus_tag="ACHKTW_005116"
     CDS             2556..>2919
                     /locus_tag="ACHKTW_005116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA circularization N-terminal domain-containing
                     protein"
                     /protein_id="HFO6465969.1"
                     /translation="MASTDTPGRGSFRGVPFLVYQEQRERGGRNIVRREYPLRESGGA
                     DDLGPKLPEFTFTVLVTGDDLQTQRSRLRDALRAPGAGELMHPDYGTLNVLINSFESR
                     YNASEQGTVEFTINVIPAS"
BASE COUNT          605 a          792 c          962 g          560 t
ORIGIN      
        1 gggctggctg aacagctggt gaacggctat gtggatctgc tcgacggcct gccgttcggt
       61 gaatcccgtg aataccgcgt caccttccgc gaactgacgg cgaaagacag cattgatgcg
      121 gagagtgagg ccgagcgtct tatggaaacc cgaaacggtc cggttctggt ggcctctccg
      181 gcactgcgcg gcattgcgct gctgcgccgt cagattgccg ctgtcggcca gattgagggg
      241 ccattatctc cgcgtcagat cggacagctc agcgagcgcg atctctcccg tctgatggcg
      301 gcggtaagcc tgcttgattc cgcgatggca ggaaagctgg tcgctgaacg ggggcgacca
      361 gatgcagtgc cgggatcaga ttgaagaagc cgcaattgtg ctggggatgg tgacaaaaag
      421 cggcccggag tgggcgctta acctcccctt atcgcagctt taccggcact gccgacagac
      481 cgaaaaaatt ctcagaacga agcagtaacc catggcaaaa aacctgaaag cctccctgat
      541 tgtcgattta ctcggcaata tttccgccaa atcccgccag tggtcacagg agctgggggc
      601 gttctcccgt tccggcagga caggcctggg tggtcttgga aatgcagccc gccgggccgg
      661 tcaggaaaca gaacttgtcg gctcccgtat gcagcgaacg cttgccggtg ttcgtggcag
      721 catccgcacg gtaacgtcag attttgaccg cctgcagggc agcattaccg gcaccatcgg
      781 gcggataagc aacctttacg ggatgctggc gggaggggct gcggtctacg gttttaataa
      841 agcctttatc cgcccggccg cagagatgga gaactatatt ctgcgtctca acgccattaa
      901 tcatggcgat aaggcaaaaa cggaagcagt gaaagcctgg gcagtacaga acgccaaaga
      961 caccacctgg gggctggcgg gggttatgca ggaatacgcc tccagtcgcg ggtttggcat
     1021 gagtgacagg gaagcccgtc gctttattac catgcttcag gatcagggcg gctatcacgg
     1081 ctggtcactg tcagatgcac agggtgcgtc cctgcaactc aaacagatgt ttgcccgcca
     1141 gagtatccag gcagcggacg ccaatattct gaccggatac gggattaacg tttaccagtt
     1201 actggccgat aagctgggcg taaaccagaa gctcatccgt gagaaaggcg aaaaaggaaa
     1261 actggggccg gacagcattc gcctgttatt tcaggtgatg gccgaacagg caaaaggtgc
     1321 ccagaaaaac gccatgaatt cctggacggg aatgacgtcc atgatgggcg acgtctggga
     1381 tcagtttgcc cgtgaagtga tggcaaaggg accgtttgac agtctgaaaa agagcctgaa
     1441 aggcttcctg gattatgctg atgcggcaca gaaaagcggc ttacaggata agctggccac
     1501 acagacggcc tcggccctga atcagggatt cgagtatgcc agagacgctg caaccggctt
     1561 ttaccgtgcc attcagaaag tcagggaaac gcttcaggca ctgcgcgacg ccggttatgg
     1621 cgatgcactg gatcgtattg gtcagggggc gcagaccgcc gcaaaatacc tgatgtatat
     1681 gtatctcgcc tcccgcgcgc tgaaggtgct caggtttgcg ggggcgggag cattacgtct
     1741 cggcgcaacc cccttacgct atggtatggc gatgacctcc gtgctgacgt cgcccttccg
     1801 caaaccgcag accactgtgc cgggaacgca gcccggacgc gctggccgct tcctgaattt
     1861 ccttaccggg gtaaatcctg ctgccgttca gcccgtgctg gtcaccaact ggcctgcggg
     1921 tggcctggcc tcaggcggcg gggatgtggt ggtcagcggt gacggcaaaa ccgtgcgcga
     1981 ccgtaaaaaa cgcggccccg gacgcgggcg gggtgtcacg actgtcgtga ccgccggtga
     2041 gcagctggcg gagagcgccg gtaaacaggg cttcttcggt cgtatgatga gccgtgccgg
     2101 tggtctgctt tcggcggccg gaaaccgcat ggggctgggg cgttttgccg ggctgttccg
     2161 tggtgccggt cggctgggtg ggggtgccct gtgggccggt gccatggcgg ctcctgtact
     2221 gctggacagc agcgccagtg cagccgataa agccggtgcc gtgggttccc ttgccggaag
     2281 tatcgccggt ggcgcgctgg gggcggctgc cggtccggtc ggggttgcca ttggttccac
     2341 agtgggcagc tatctcggtg attatctggg gggctggctg acgcaggcct ggcagaaact
     2401 gcgtggcagc agtgacgaaa acggcggaca ggccaccgcg aaaaccgccg cacgggtaga
     2461 gcttgtggcc cctgaaggct ggcgggcgcg cagtattgat gtggatgata ccgcacagca
     2521 tggcctggat gtgaacgtct ggaacggagg gaactatggc ctctactgac acgccggggc
     2581 gtggctcctt tcgtggcgta ccgttcctgg tttatcagga gcagcgggaa cggggcggac
     2641 gtaacattgt gcgccgtgaa tacccactgc gtgaaagcgg cggtgcggat gatctggggc
     2701 caaaactccc ggagttcaca ttcacggttc tggtgaccgg agacgatctt cagacacagc
     2761 gaagccgcct gcgtgatgcg ctgcgtgcac ccggtgccgg tgaactgatg cacccggatt
     2821 acggcacgtt aaacgtgctg ataaacagct ttgaaagccg ttataacgcc agcgagcagg
     2881 gaacggttga gttcacgatc aacgtgatac ccgccagtg
//
LOCUS       DAZFQD010000260         2893 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.263, whole genome shotgun sequence.
ACCESSION   DAZFQD010000260 DAZFQD010000000
VERSION     DAZFQD010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2893
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.263"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(122..544)
                     /locus_tag="ACHKTW_005117"
     CDS             complement(122..544)
                     /locus_tag="ACHKTW_005117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mor transcription activator family protein"
                     /protein_id="HFO6465970.1"
                     /translation="MAETQMSMFGDSEQLHALIDRLDDIPDDELKKNWPGTLRDLVDV
                     ISAELHRQGIEPAQAGMLARKVAAAQAGYMGGRGYYLPVGESLFTELRNNEIFSRWSQ
                     GEKIETLRRHYRMSETQIYTVIREQRRLHLARTQPPLF"
     gene            complement(650..886)
                     /locus_tag="ACHKTW_005118"
     CDS             complement(650..886)
                     /locus_tag="ACHKTW_005118"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6465971.1"
                     /translation="MDIFLYSGGTLSGRLKLFVFALEQAWCALRVGIIGFHVDETTIT
                     RNMHKLFDKKNSFRVCIFKNKNDLYHIVTPENDQ"
     gene            885..1307
                     /locus_tag="ACHKTW_005119"
     CDS             885..1307
                     /locus_tag="ACHKTW_005119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6465972.1"
                     /translation="MKQPPSLLLSCPPELAHELNDLAKEYKLTNHNIEAATLSAAGSD
                     IVLIVLAAASCDGFWQALASMFQTWLHRKPTKQTPPEKKVIRSVIVRSWEHYQETTKQ
                     TEFYNFDADELAKLIALYEKPDETIGCIIPSETEIQEK"
     gene            complement(1334..1843)
                     /locus_tag="ACHKTW_005120"
     CDS             complement(1334..1843)
                     /locus_tag="ACHKTW_005120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gp16 family protein"
                     /protein_id="HFO6465973.1"
                     /translation="MRGKLISAIHVAKRELALDDETYTSALLAATGKASCRDMSPDEL
                     SRVLDVFKKRGFKVRQKPVNRALKPGTVTAKIRAIWKVMHRQGFISDGAETALNRWVQ
                     SQTAAQNGGEGVANWQWLEQHPELASDVLERLKRWHRRKMLAALDMSERAVMSYERVC
                     RLYEKSFPC"
     gene            complement(1840..2151)
                     /locus_tag="ACHKTW_005121"
     CDS             complement(1840..2151)
                     /locus_tag="ACHKTW_005121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6465974.1"
                     /translation="MDEQITDNISLLFWCLYITSFFGAFVITRWLCRKIISLFDKRYL
                     LELAADALIQQAIVLYSGEFFCRITTRDGWHIMIIPPTHHIRWNEPEKAFHVRKKVNT
                     V"
     gene            complement(2144..2476)
                     /locus_tag="ACHKTW_005122"
     CDS             complement(2144..2476)
                     /locus_tag="ACHKTW_005122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6465975.1"
                     /translation="MITTLFVESDEPLVCAAGMPVCGGTLTGVYFGDLRGYPWHSLND
                     AFPPDMEAVVLIVQYGHRQELRIGHIGYEGFFVDEETGACLEDEDGQVTHWCHISALP
                     ELREIHNG"
     gene            complement(2473..>2893)
                     /locus_tag="ACHKTW_005123"
     CDS             complement(2473..>2893)
                     /locus_tag="ACHKTW_005123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000973023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AsnC family protein"
                     /protein_id="HFO6465976.1"
                     /translation="KRSFWGVHNRIVLLRGTYPELLKCKRPRFKHDEDKFIRKNARTM
                     TGKQIGEYLGRDRDSVHNRARYIGVSMKKYGELLPFTRIPDDDVHLIRELRDAESPRR
                     LTFREIGEKFELSESTVNFIYHHRRTAGDAVLRELTP"
BASE COUNT          770 a          726 c          651 g          746 t
ORIGIN      
        1 agaacaaggg caatcagctt tttacgcagt ggtgcgggga gttttggcat tgttacgctg
       61 tctcatgatg ttctctgagt cagcgtaacg gtgaaggaaa ggcggcaggc tttgcggaat
      121 atcagaaaag tggcggttgc gttcttgcca gatgcagacg gcgctgttcg cgtatgacgg
      181 tataaatctg ggtttctgac atcctgtaat ggcggcgcag ggtttcaatt ttttcgccct
      241 ggctccagcg ggaaaaaatc tcattattgc gcagttcggt gaaaagggat tcaccgaccg
      301 ggagataata accacgcccg cccatgtacc cggcctgtgc ggccgcaact ttacgggcaa
      361 gcattcctgc ctgagcaggt tcaatcccct gacggtgcag ttcagcactg atcacatcaa
      421 ccaggtcccg cagcgtgccg ggccagttct ttttcagctc atcatcagga atgtcatcca
      481 gacggtcaat cagcgcgtgc agctgttcgc tgtcaccaaa catgctcatc tgtgtttcag
      541 ccatacccgc ctccggtcat cattacacag ggcaagttta aaataaaaat cccgcgctgt
      601 ggcgggattt agaggcgctg taaatcataa cccgaaatga gctgtgtatt tattgatcat
      661 tttccggtgt aactatatga taaagatcgt ttttattttt aaaaatacaa accctgaagg
      721 agtttttctt atcaaaaagc ttgtgcatat tgcgggttat tgttgtttca tcaacatgaa
      781 aaccaattat cccaactctt aaagcacacc aggcttgctc cagtgcaaaa acaaataatt
      841 tcagtctgcc tgacagcgtt ccaccggaat aaaggaagat atccatgaaa caacctccat
      901 ctttgctttt atcgtgccca ccagaactgg cccatgaact caatgatctg gcgaaagagt
      961 ataaacttac aaatcataat atagaagcag caacacttag tgctgccgga agcgatattg
     1021 tattgatcgt tctggccgca gcttcatgcg atgggttctg gcaggcatta gccagtatgt
     1081 ttcagacgtg gttacatcgt aaaccaacaa aacaaacacc cccggaaaag aaagttattc
     1141 gtagcgtcat tgttcgtagc tgggaacact atcaggagac aactaaacaa actgaatttt
     1201 ataactttga tgctgatgag ctggctaaat tgatagcgtt atatgaaaaa ccagatgaaa
     1261 caattggttg cataatcccg tcagaaacgg aaattcagga aaaataaaat cccgcactgt
     1321 ggcgggattt gggttaacag gggaatgatt tttcatacag cctgcaaacg cgctcataac
     1381 tcatcactgc acgctccgac atatccaggg ctgccagcat tttgcggcgg tgccagcgtt
     1441 tgagacgctc cagcacatca gaggccagct cggggtgttg ctccagccac tgccagttag
     1501 ccacaccttc gccgccgttc tgcgcggccg tctgcgattg cacccagcgg ttaagggccg
     1561 tttccgcacc atcagaaata aagccctgcc ggtgcatcac tttccagatg gcgcgaattt
     1621 tggcggtcac cgtacccggc tttaaggccc ggttaaccgg tttctgacgc actttaaaac
     1681 cgcgtttttt gaaaacatcc agtacgcggg ataactcatc cggtgacata tcccggcaac
     1741 tggctttacc ggtggctgcc agcagtgcgg atgtataggt ttcatcatcc agagcaagct
     1801 cgcgttttgc cacatgaatg gcagatatca gttttcctct cataccgtat ttaccttttt
     1861 acggacatgg aaagcctttt ctggctcatt ccaccggata tgatgcgttg gcggaataat
     1921 cattatgtgc cagccatctc tggttgttat tcggcagaaa aactcaccgc tgtataacac
     1981 aatggcctgc tgaataagcg catccgcagc cagctcaagc agatatcttt tatcaaaaag
     2041 gctgataatt ttccggcata accaccgggt gataacaaat gcgccgaaaa acgatgtgat
     2101 atataaacac cagaacagca gagaaatatt gtccgttatt tgctcatcca ttgtgtatct
     2161 cccgtaattc cggtaaagcc gaaatatggc accagtgcgt cacctgtcca tcctcatctt
     2221 caaggcaggc tccggtttct tcatcaacaa aaaaaccttc atacccgata tggccgatgc
     2281 gcagctcctg tcggtgaccg tactgaacaa tcagtacaac agcctccata tcaggcggga
     2341 aagcatcatt cagtgaatgc cagggataac cgcgcaaatc cccgaaataa accccggtca
     2401 gggttccgcc acagaccggc atcccggcgg cacataccag aggttcgtct gattcaacaa
     2461 ataaggtcgt tatcatggcg ttaactcccg taatacagcg tccccggcag tccggcgatg
     2521 gtgataaata aaattcaccg tactctcgga taattcaaat ttctcgccta tttcccggaa
     2581 ggtaagacgt cgtggtgatt cagcatcccg taattcacga ataagatgaa catcatcgtc
     2641 aggtatacgg gtgaagggca gcaattctcc gtattttttc atgcttacgc caatatatcg
     2701 cgcccgattg tggacagaat cccggtctcg ccccagatat tctcctattt gcttaccggt
     2761 catcgttctg gcattttttc ggataaattt atcttcatca tgtttaaatc ttggacgttt
     2821 gcatttgagc aactccgggt aagtaccgcg taataaaaca atccggttat gtacacccca
     2881 gaaacttctt ttt
//
LOCUS       DAZFQD010000261         2861 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.264, whole genome shotgun sequence.
ACCESSION   DAZFQD010000261 DAZFQD010000000
VERSION     DAZFQD010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2861
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.264"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(110..1126)
                     /locus_tag="ACHKTW_005124"
     CDS             complement(110..1126)
                     /locus_tag="ACHKTW_005124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HFO6465977.1"
                     /translation="MKNPDSPVLSLRDYSTRDTKWDSDRVMADRVAQIYENDSMFSSR
                     GERMFDCSRRLLFAPKVSRLTGEMKLALRKGEFCHVPFCPVCSRRRSLRWMRRLWEAL
                     PKLLVERPAARWLFMTLTVKNPPVENTRETLIRMNAAWKRLSDRKEFRSVLGWLRTTE
                     ITYGKVPGCCHPHFHVLMMVPPSMLSGNGYVKHARWVEIWSECLRVDYEAGVDIRVVK
                     PKQGWKRPDGVTLPDMHRAALESGVIETMKYTVKSSEVVRDPAWFLELARQTYGLRMV
                     ATGGRLKEVLKVDKPETDEDLVGADIPAEPDEFEEQAFWLAFDWWRDEKRYKRNPKAD
                     KKKD"
BASE COUNT          712 a          849 c          652 g          648 t
ORIGIN      
        1 aaaaaaaacg actaaatcct tgcccgtttc tgaaaatcac attagaacgc ttctgagcgc
       61 gtttctgaat attctcccag tgcatgaata gttcgcgcct gaaacgcgct caatccttct
      121 tcttgtcagc tttcggattc cgcttatagc gcttctcatc ccgccaccag tcaaacgcca
      181 gccagaaggc ttgctcctcg aactcgtccg gttctgccgg aatatctgcc ccaaccaaat
      241 cctcgtctgt ctccggttta tcgactttca gaacctcttt gagacgaccg ccggttgcca
      301 ccatccgaag cccgtaggtc tgccgcgcca gctccagaaa ccacgccgga tcacgcacaa
      361 cttccgaaga tttcaccgtg tatttcatcg tctcaatgac gcctgactcc agcgccgcac
      421 gatgcatatc aggcagggtt acaccatccg gccgtttcca gccctgtttc ggttttacca
      481 cccgaatatc cacacccgcc tcataatcga cacgaaggca ttcactccag atctccaccc
      541 agcgagcatg tttcacataa ccattaccgg acaacatcga aggggggacc atcatcagaa
      601 cgtgaaaatg cggatggcaa catcctggca ccttcccgta agtaatttcc gtcgtacgta
      661 accagccaag aaccgacctg aattccttac gatcactcaa gcgtttccat gctgcattca
      721 tccgaatgag tgtttcgcgg gtattttcca caggaggatt tttgacagtc agcgtcatga
      781 acaaccagcg ggccgcagga cgttcaacca gcaattttgg caaggcctcc cagaggcgac
      841 gcatccagcg gagagagcga cgacgactac acaccggaca aaacggaaca tgacaaaact
      901 cccccttccg aagggccagc ttcatttcgc cagtcaggcg ggacaccttc ggcgcaaaca
      961 acaaccgacg ggaacaatca aacatccgtt ctccacgaga ggaaaacatc gaatcatttt
     1021 cgtaaatctg cgccaccctg tcagccatca ctcgatcgct gtcccatttt gtatcccgcg
     1081 tgctgtaatc acgtaacgag agtaccggac tgtctggatt tttcatttgc accccccaag
     1141 aactgggcag atgcggaaat tttgtgttag catttttctg tcacctgtga ctctgttgcc
     1201 gaacaacgaa ttccaaactg gtgatcgacc cctggagtgt taccgcactc gcagggaccc
     1261 ctccccgagc tgtttatttt ttaaccatct ttttttatct cttttccatc tatttttacc
     1321 gctcaaagcc gcgccaccac tgggctaagc caaaataaca cagaccgcag ttgttgccta
     1381 tagtatatat acaaggaaac gcgcgcgaag acctcgccaa ggtagactat tttttgttcg
     1441 attttccgag aacaaaaaca acaaccacga aagcgttttc ccgacaaaac acccacaggc
     1501 ttttccaccg attttgtgaa caacaaaaaa cctgcctcgc aggtggttgg tcccactgcg
     1561 tgacaccaac caccatcacc agtgtttttt ctcgtgccgt cgtcaaacac aaaatcggaa
     1621 cccacgccgg aggcgcggga ccccacgatt ttctgtttga cgagtcataa aacgtctcac
     1681 gctgacagat ttcaatctga ctgcgtgaga cgttggcggg gtgtcggggc gcagccctga
     1741 ccaggtggca tttgtctgat tgcgcgtgcg cggtccgaca aatacacatc ctgtcccgac
     1801 tttttctccg aaatgctcgc agaggggcgg cagttttgtg ccgttcagcc ctccgcagga
     1861 gcgcgctgtt tctcgacgaa ggagagaaac gtctaagtac gcatccgcgc ttaacgcgcg
     1921 gatgattttt ggcaccgcag ccaccacggc cgcgcaaaat tctggcgaac ggacagcaaa
     1981 tcattttgcc gtttttgtgc cgcagccgtt ccgcgcgatc gacaaactgg cagtcggggg
     2041 aatctcacga agtgcacgaa gtgcgcactt atacaatctc aacttcgttt cgattgtgtg
     2101 cggtctgcga cgcggtgaaa accgcagata acaggcaaaa aaatccccct gtgtcagggg
     2161 gccgtcaaag tcaatttcct gatgatgata gttattttga ggtgttgcta tgcccgctca
     2221 atttgccgca ggaacaccgc aaaaacaact ccgatgacgg gacaggatgt gggaatgtcg
     2281 gaccgcgcac gcgcactcag acattcccca cctggtcagg gctgcgcccc gacaccctcg
     2341 acaacctgcc cgggacacgt taaaacgaca tctgcgcgtt tctggagcgc ccgttaacca
     2401 tggggatggt attagcgccg ggatgcgaca gaaacgcgct taaaacgccc ctgaacgcat
     2461 caaacgcgaa aaaacgaata aaaccttgcc tgtctgcaaa aatcgcgtta gaacgcgtct
     2521 gagcgcgtct gaggggttaa aattcactgt gcgtcaaatg gggattgtta caccccgccc
     2581 tcaaacgcgc tcacttcccc ttcccgtcta catgcggaaa tgttgttgtt ttgtatcttt
     2641 tatgttaagc atcggatgag cgtgtttctt atcgcatttc cactatcatc aacccgcgaa
     2701 gacctgtaga aacacgctca ccccccccga ctactggaca gcagaatcgc accgcgttaa
     2761 acatgacatg cgcgtttctg gtgagcgaaa agctgtcagc cgtggattgc cgtagcagag
     2821 acccagaaac gcgcttaaaa cgcttccgag tgcgccaaaa c
//
LOCUS       DAZFQD010000262         2756 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.265, whole genome shotgun sequence.
ACCESSION   DAZFQD010000262 DAZFQD010000000
VERSION     DAZFQD010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2756
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.265"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            517..660
                     /locus_tag="ACHKTW_005125"
     CDS             517..660
                     /locus_tag="ACHKTW_005125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2627 domain-containing protein"
                     /protein_id="HFO6465978.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            673..882
                     /gene="cspE"
                     /locus_tag="ACHKTW_005126"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             673..882
                     /gene="cspE"
                     /locus_tag="ACHKTW_005126"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HFO6465979.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            1048..1857
                     /gene="rlmA"
                     /locus_tag="ACHKTW_005127"
     CDS             1048..1857
                     /gene="rlmA"
                     /locus_tag="ACHKTW_005127"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HFO6465980.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMIQARRAFLDAGHYQPLRDAIVAQLRERLDEKATAVLDIGCGEGYYTHA
                     FADVLPEITTFGLDVSKVAIKAAAKRYPQVTLCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSDELCY
                     PMRLRGDEAVALLQMTPFAWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(1854..2420)
                     /gene="mntP"
                     /locus_tag="ACHKTW_005128"
     CDS             complement(1854..2420)
                     /gene="mntP"
                     /locus_tag="ACHKTW_005128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HFO6465981.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGATLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
BASE COUNT          739 a          629 c          642 g          746 t
ORIGIN      
        1 aagatatttt cctcattaat ttattaggat gtttacatcg gatttgtgat taagcgtggt
       61 attatttatt agcgaaacgt ttctctcttg atttttttgc tcattcatca atttttctta
      121 ttttaaattt tcaatccctt tggggattga cttctcttta gggtaattga tagccgttaa
      181 ctgactgttt tatgagaaaa agtgatataa ctttttattc attgcatagc aaaaaatgtg
      241 atattgcacg cactatgtaa taacttctcc cactgacctg gaacaactga acttattgaa
      301 ctatgttaga aaatacgcca gtttaagtat ctgcctgaac tggcaaggtt aagcacaatg
      361 atatatcggc gcgtattccg ttgcataagt gtgcaaaaaa agtggaagac gtatcgagat
      421 ttgtgcgtct gatcgagaca tgtttaaaaa tggcttgcca taattaacgt ggtatgtgat
      481 aacagatttc gggttaaacg aggtacagtt ctgtttatgt gtggcatttt cagtaaagaa
      541 gtcctgagta aacacgttga cgttgaatac cgcttctctg ccgagcctta tattggtgcc
      601 tcatgcagta atgtgtcagt tttatctatg ttatgcctgc gggcgaagaa aacaatctaa
      661 ggaatttttc aaatggcaaa gattaaaggt caggttaagt ggttcaacga gtctaaaggt
      721 tttggcttca ttactccggc tgatggcagc aaagatgtgt tcgtacactt ctccgctatc
      781 cagggtaatg gcttcaaaac tctggctgaa ggtcagaacg ttgagttcga aattcaggac
      841 ggccagaaag gtccggcagc tgttaacgta acagctatct gatcgaatcc actgatctga
      901 agtgtgaata cgcttcaatc tcgctataaa gcctcgtcga atgcgaggct ttttactatg
      961 ctttatcttc gctcctggcg ttcggatatt tgcccgccgc gtgattcgcg ttacacttgc
     1021 ggcctttagt atcctgccgg agttgtcatg tcgttttcct gtccactttg ccatcagcct
     1081 ctttcgcgtg aaaaaaacag ctatatctgt ccccagcgac atcagtttga tatggcgaaa
     1141 gaagggtatg tcaatctgct gcccgttcag cataaacggt ctcgtgatcc gggcgacagc
     1201 gcggaaatga tacaagcacg ccgcgcattc ttagatgccg gacattatca gccgctgcgt
     1261 gatgcaattg tcgcccaact gcgggaacgg cttgatgaaa aggccacggc ggtgctggat
     1321 attggctgtg gtgaagggta ttacacacac gcatttgccg atgtgttgcc cgaaatcacc
     1381 acgtttggcc tggatgtctc gaaggtagcg ataaaagcgg cggcgaaacg ctatccgcag
     1441 gtcactcttt gtgtcgcttc cagccaccgt ttgccgtttt ccgataccag tatggacgcc
     1501 ataatacgta tttacgcgcc gtgtaaagca gaagaattag cacgagtagt gaagcccggc
     1561 ggctgggtca ttactgccac gccgggaccg cgacatttga tggagctgaa ggggctgatt
     1621 tacaatgaag tacatcttca tgcacctcat gcagaacaac tggaaggttt tacattacag
     1681 cagagtgatg agttgtgtta tccgatgcgt cttcgcggtg atgaagccgt cgcattattg
     1741 cagatgacgc cgtttgcctg gcgtgcgaag ccagaagtct ggcaaacact ggcagcaaaa
     1801 gaagtgttcg actgccagac ggactttaat attcacctct ggcagcgttc ttattaaccg
     1861 tggaagtgcg tccagaggat ctggacgccg atgccgatca gcaccagccc gccgagaatt
     1921 tccgcttttt tcccaataat tgagccgata aagcgaccaa ccatcatccc taatgtcgac
     1981 ataatcaagg ttgcacaacc aatggccaat gcggtcgcga taatgttgac ctgcaggaaa
     2041 gcaagaccaa cacccacagc catggcatcc aggctggtgg caatcgcggt ggttaccagt
     2101 agccagaaac cgtgccgacg gcgcggctct tcatcttcat catctgcgcc acgaaaaccc
     2161 tcaataatca ttcgcccgcc gaggaatatc agcagcacaa acgcaatcca gtgattccat
     2221 tcaaggacaa accggctggc taacatgccc attccccagc cgatcagcgg cgtcagggtt
     2281 tcgacggcac caaaaataag gccggttcgc aatgcttcag aaaatttcgg tttatggagg
     2341 gtggcacctt taccgattga tgcagcaaat gcatccatcg acataccaaa cgcaagaaga
     2401 acagtagcag tgatattcat gacaacgtcc tgaccggggt atccataaaa cacatcatcg
     2461 cccccagcaa acatgcatac atgaacaaca agccgcgatg gttttcgcgt tcatgcaggc
     2521 atagcgattg atgtgcatat ggtctcgcct gactgaaagt attcagtccg tacgtgccac
     2581 gttttgcaac gagtatgttg acacgtacat ttccggaatc tggaaatcgg ctactcccca
     2641 aatgagggcg caaccttaac atattttgag aatataaaac aactgttgga atatattatt
     2701 tcattgcttt attgataacg atttacattt agatttatat atcaatgaaa cgttaa
//
LOCUS       DAZFQD010000263         2652 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.266, whole genome shotgun sequence.
ACCESSION   DAZFQD010000263 DAZFQD010000000
VERSION     DAZFQD010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2652
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.266"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..953
                     /locus_tag="ACHKTW_005129"
     CDS             <1..953
                     /locus_tag="ACHKTW_005129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="terminase family protein"
                     /protein_id="HFO6465982.1"
                     /translation="EGLTRTYFSTPSGETHEAYQFWTGDRWNSEQPRSKRSEFDVSHA
                     HLKGGVLFPDETWRQIVTIEDAVANGWRYTNIGVIRSENTESEFRNLYMCEFVRDGEA
                     AFSLSALTGCGVDGYDEWPDWKPFAARPMGVREVWLGYDANGSSGKGDCGALSVCVPP
                     LVAGGRFRTVETVRVQGMEFEQQAALIEEYTQKYNVKHIGIDVTGGNGEGVYQIVKKF
                     FPMAVPYNFNIASKRALVMKMLQVVRAGRWEYDRSERDLIQAFASVRKIQTPGGYITY
                     DTDRSRGVSHGDLAWATMLAVINEPIGAEREDNRGFAMEF"
     gene            959..2002
                     /locus_tag="ACHKTW_005130"
     CDS             959..2002
                     /locus_tag="ACHKTW_005130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO6465983.1"
                     /translation="MSKRKKYTGKRGQDSLHDALKSTPALSALTFDGPYEVSRMDLLD
                     NMSCADNGRYFDTPMDWNAIARASRRASWHQSALYFKRNALNGCFVPHPLLSRQAFSA
                     FALDWFVFGNAYLEVRRNRLGEPLLLRPALAKYTRRGSDLDTYWYLNDDGTEFSFRKG
                     TVCHVLNPDINQEIYGMPEYIGGLLSVSLSNSADTFRKLYYDNGSHAGCIIYVGTSQA
                     NAESVEAIKKTLTDSRGRGAFKNLFLHAPGGGKDGVQILPFQQITAKDEFLNIKGSAR
                     DDILAAHRVPPQLMGAMPDGNAAFGDVEKAARVFFINELQPVMEAMKHVNEWLGVEVM
                     RFNPYALLLDNAS"
     gene            complement(2068..2571)
                     /locus_tag="ACHKTW_005131"
     CDS             complement(2068..2571)
                     /locus_tag="ACHKTW_005131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000017881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6465984.1"
                     /translation="MSHFRDLYLANKKSRDARREVTGILRNQAEKLVAFYEQWLELPS
                     ETWEDKDGEHHRYVDTGFPCRNDRDFRPFPVNMIATDTDNIFRMAVRTWVDSHDEGES
                     VNIVLAMELHSVGKDGVSLRVRVEDDTSIRVVINGDGDREWLPIVNSMKIHIISKLQR
                     RTPSAFR"
BASE COUNT          597 a          679 c          724 g          652 t
ORIGIN      
        1 tggaagggct tacgcgaacc tacttttcaa caccgtcagg tgaaacacat gaggcgtatc
       61 agttctggac gggggaccgc tggaacagcg aacagccacg ctcaaaacgc agtgagtttg
      121 atgtttcaca tgcgcatctg aaggggggcg tgctctttcc tgacgaaaca tggcggcaga
      181 ttgtcactat tgaggatgct gtcgctaacg gatggcggta caccaatatt ggtgttattc
      241 gcagcgaaaa tacagaaagt gaatttcgta atctgtacat gtgcgaattt gtccgtgatg
      301 gtgaggcagc cttcagcctg agtgcgctca ccggctgcgg tgttgacggc tatgacgaat
      361 ggcctgactg gaaacctttt gctgcccgcc ctatgggggt gagggaggtc tggcttgggt
      421 atgacgccaa cggcagcagc gggaaggggg actgcggggc actgtcggtg tgcgtgccgc
      481 cccttgttgc cggtgggcgt tttcgcacgg tggaaactgt cagggttcag gggatggagt
      541 ttgaacagca ggccgcactg attgaggaat acacccaaaa atataacgtg aaacatatcg
      601 gcattgatgt gaccggcggg aacggggagg gggtttatca gattgtgaaa aagtttttcc
      661 cgatggccgt accgtacaac ttcaatatcg cctccaagcg ggcactggtc atgaaaatgc
      721 ttcaggtggt gcgtgccgga cgctgggagt atgaccgcag tgaacgtgac ctgatccagg
      781 cattcgcatc ggtgcgaaaa atacagacac ccggcggcta tatcacctac gacaccgacc
      841 gctcccgtgg cgtcagccat ggtgacctcg cctgggcaac catgcttgct gttatcaatg
      901 agccaattgg tgcggagcgt gaggataatc gcggctttgc aatggagttc tgaacacaat
      961 gagtaaacgc aaaaaataca ccggaaagcg ggggcaggat tcgctacacg atgcactgaa
     1021 atcaacgcct gcactcagcg cattaacctt tgatgggccc tatgaagtca gccgcatgga
     1081 tctcctggat aacatgtcat gtgcagataa cgggcgctac tttgataccc ccatggactg
     1141 gaacgctatc gcccgtgcat cccgtcgtgc gtcctggcat cagtcggctt tgtacttcaa
     1201 gcgcaacgcc ctgaacgggt gctttgttcc tcaccctctg ctgtcccgcc aggcgttttc
     1261 agcgtttgcg ctcgactggt ttgtgtttgg taatgcctat cttgaagtgc ggcgtaacag
     1321 gctgggggaa ccactcctgc ttcgcccggc actggcaaaa tatacacgca ggggaagtga
     1381 cctggatacc tactggtatc tcaacgacga cggtacggag ttcagtttca ggaaagggac
     1441 tgtgtgccac gtgcttaatc ctgatattaa tcaggagatc tacgggatgc cggaatatat
     1501 cggcgggttg ctgtctgtca gcctgtcaaa ctcagcggat acattccgca agctctacta
     1561 cgataacggc tctcatgccg ggtgtattat ctatgtaggc acatctcagg cgaatgcgga
     1621 aagtgtcgag gccattaaaa aaacgcttac ggattcccgt ggtcgcgggg cgtttaaaaa
     1681 cctgtttcta catgcgccgg gtggtggcaa ggatggtgtg caaatactgc cgttccagca
     1741 gataaccgca aaggatgaat tcctgaacat caaggggagc gcccgcgacg atatccttgc
     1801 tgcccatcgt gtaccacctc aactgatggg ggcaatgcct gacgggaatg cggcgtttgg
     1861 tgacgttgaa aaagcggcga gagtgttttt catcaacgag ttgcaacctg tcatggaggc
     1921 catgaagcat gtcaatgaat ggcttggcgt tgaggtgatg cgctttaacc cctatgccct
     1981 gcttctggat aacgccagct gacgcgcttc gcttgtctgc tgcttcgccg gggcataaaa
     2041 aatttatgcc ccgactctcc agctctctta acggaatgcg gatggcgttc ttctttgcag
     2101 cttgctgata atatgtatct tcatgctgtt tactatcggc agccattccc tgtcaccgtc
     2161 tccgttgatg accacgcgaa tggaggtatc atcttccacg cgaacccgaa gagatacacc
     2221 gtcttttccg acggaatgta attccattgc caggactatg tttaccgact ctccttcatc
     2281 gtgtgagtca acccatgttc ttacagccat ccggaatatg ttatctgtgt cggttgcaat
     2341 catatttacc ggaaatggcc tgaagtcccg atcgtttctg cacggaaacc ccgtatcaac
     2401 gtaacgatga tgctcgccgt ctttgtcttc ccatgtctct gaaggcaatt ccagccactg
     2461 ctcataaaaa gcaaccagct tttcagcctg attgcgcaga atccctgtaa cctcccgcct
     2521 cgcgtcccgg ctctttttgt ttgccaggta caaatcgcga aaatggctca tgtcgctctc
     2581 ctttggtctg tttatgccag cctgtattct gcgctcacct catattgtgc gcaagttttc
     2641 acccgtgaat ga
//
LOCUS       DAZFQD010000264         2648 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.267, whole genome shotgun sequence.
ACCESSION   DAZFQD010000264 DAZFQD010000000
VERSION     DAZFQD010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2648
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.267"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            45..746
                     /locus_tag="ACHKTW_005132"
     CDS             45..746
                     /locus_tag="ACHKTW_005132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFO6465985.1"
                     /translation="MKTCITKGIVTVSLTAILLSCSSAWAAGKGGIGLAATRLVYSEG
                     EEQISLGVRNTSPDVPYLIQSWVMTPDNKKSADFIITPPLFVLNPANENLLRIMYIGA
                     PLAKDRETLFFTSVRAVPSTTKREEGNTLKIATQSVIKLFWRPKGLAYPLGEAPAKLR
                     CTSSADMVTVSNPTPYFITLTDLKIGGKVVKNQMISPFDKYQFSLPKGAKNSSVTYRT
                     INDYGAETPQLNCKS"
     gene            771..2648
                     /locus_tag="ACHKTW_005133"
                     /pseudo
     CDS             771..2648
                     /locus_tag="ACHKTW_005133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706863.2"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
BASE COUNT          739 a          569 c          650 g          690 t
ORIGIN      
        1 agtgttctgg aggggatatt catatttttt aagagtgact atttatgaaa acttgcataa
       61 caaagggaat tgtgaccgta agtttaacgg caatattact cagctgctca tccgcatggg
      121 cggcaggtaa aggcgggatt ggacttgcag caacacgtct tgtttattca gaaggtgaag
      181 agcaaatttc actgggtgta cgtaacacca gtccggatgt tccttatctt attcagtcat
      241 gggtgatgac cccagataat aaaaaatcag cagattttat tattacacca ccgctatttg
      301 tgctgaatcc ggctaatgag aatctgttac gcattatgta cattggtgcg ccgttggcga
      361 aagacagaga aacccttttc ttcactagcg tacgggcagt cccttcaaca acgaagcggg
      421 aagagggaaa taccctgaag attgccacac aaagcgtcat caaacttttc tggcgaccaa
      481 aaggtttagc gtatccctta ggcgaggctc cggcgaaact gcgttgcact tcgtcagctg
      541 acatggttac ggtcagtaac ccaacacctt atttcattac cctgacagac ctgaaaatag
      601 gtggaaaagt agttaaaaat caaatgattt ccccctttga taaataccaa ttttctctgc
      661 caaagggggc caaaaatagc agcgtaacgt atcgaaccat caatgactac ggggcggaaa
      721 cgccgcaact caactgtaaa tcgtaagccg tcttcagtta agagagcgag atgtatagaa
      781 ctcatcgaca acacagcctg ttaagctctg gtggagtgcc atcgtttatt ggtgggctgg
      841 tggtgtttgt gtcggcagcg ttcaatgcac aagctgaaac ctggttcgat cctgcttttt
      901 tcaaagatga tccctcaatg gtggccgatt tgtctcgttt cgaaaaagga caaaaaataa
      961 cgccaggggt ttatcgtgtc gatattgttc tgaatcagac aattgtagat acgcgcaacg
     1021 tcaattttgt tgagataacg ccagagaagg ggattgccgc ctgtttgacg actgaaagcc
     1081 tggatgcaat gggtgttaat actgatgcgt ttccggcttt taaacaactg gacaaacaag
     1141 cgtgtgtgcc attggcggag attattccgg atgccagcgt aacgtttaat gtgaataaac
     1201 tccgtctgga aatttcagta ccgcaaatcg ccatcaaaag taatgcccgt ggttatgtcc
     1261 cccctgaacg ttgggatgaa gggatcaacg cgctattact gggatattcg tttagcgggg
     1321 ctaacagtat tcatagcagc gcagacagtg attctggcga cagctatttt ctgaatttaa
     1381 acagtggcgt taatttaggc ccatggagat tgcgcaacaa ttcaatatgg agtcgcagta
     1441 gtggccaaac cgcagaatgg aagaatctca gcagctattt gcagcgggcg gttattccac
     1501 taaaaggcga actgaccgta ggtgatgatt atactgcagg cgattttttc gatagtgtca
     1561 gctttcgtgg tgtgcagctg gcgtcagatg acaacatgct gccagacagc ctgaaagggt
     1621 ttgcgcctgt ggtgcgtggt atcgccaaaa gcaatgccca gataacgatt aagcaaaatg
     1681 gttacaccat ttaccaaact tatgtatcgc ctggtgcttt tgaaattagt gatctctatt
     1741 ccacgtcgtc gagcggtgat ttgttagttg aaatcaaaga agcggacggc agcgtcaata
     1801 gctacagcgt accgttttcc agcgtgccat tactccagcg tcaggggcga atcaaatacg
     1861 cggtgacact ggcgaaatac agaaccaata gtaatgaaca gcaggagagc aaatttgccc
     1921 aggccacgtt gcagtggggc ggaccgtggg gaacgacatg gtatggtggt ggacaatatg
     1981 ctgaatatta ccgtgccgcc atgtttggtc tgggatttaa ccttggcgat ttcggagcaa
     2041 tttcgttcga tgtgacccag gcgaagagta cgctggcaga ccaaagcgaa cataaaggtc
     2101 agtcatatcg ttttctgtat gccaaaacgc tcaaccaatt gggcactaat tttcaattga
     2161 tgggctatcg ctattcgacg tcgggtttct acaccctttc cgacaccatg tataaacata
     2221 tggatggcta cgaatttaat gacggtgatg atgaagatac gccgatgtgg tcgcgttatt
     2281 acaatttgtt ttacaccaaa cgtggcaaac tgcaggtcaa tatctcccag caattaggcg
     2341 agtacggttc gttttattta agtggtagcc agcaaactta ctggcatacc gatcaacagg
     2401 atcggctatt acagtttggc tacaacacgc aaattaaaga tctctcgctg ggggtttcct
     2461 ggaactacag taagtcccgt ggtcaacctg atgctgatca ggtgtttgca ctaaattttt
     2521 ccctgccgct caatctgttg ctccccagaa gtaatgatag ctataccagg aaaaaaatta
     2581 cgcctggatg acctctaaca ccagtatcga taacgaaggg cacattacac aaaacctggg
     2641 tttaacgg
//
LOCUS       DAZFQD010000265         2627 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.268, whole genome shotgun sequence.
ACCESSION   DAZFQD010000265 DAZFQD010000000
VERSION     DAZFQD010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2627
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.268"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..737
                     /locus_tag="ACHKTW_005134"
     CDS             <1..737
                     /locus_tag="ACHKTW_005134"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6465986.1"
                     /translation="TNNNLINRIDLIMKVTAPLRKKLDSAKKALEVLEPARKQVEALT
                     SSNKNEKTKSNLWYFENEILEYSEPTDQRVLHFLITKLNELKSNKTAANKFFKDCIYI
                     ALKRERERSIDDEALHRFISTGEVEAVYFLLNSNISSRESQIIISYLFVYICFIQSYI
                     DYVNEKTNINNDICIIRTNSHYQKLAVDAQKIIIRELNKKQTNYRIAKILEDELSSKY
                     KHVPSKNTFKNWIELIKKHLKQRSDK"
     gene            978..1187
                     /locus_tag="ACHKTW_005135"
     CDS             978..1187
                     /locus_tag="ACHKTW_005135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001708222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO6465987.1"
                     /translation="MAVKNDFSPDQRMTRKQAAEYIGVSEGTLGVWACTGKVKLPFYK
                     AGRCRVFYLKHELDEFLQSTRRYQS"
     gene            1265..1546
                     /locus_tag="ACHKTW_005136"
                     /pseudo
     CDS             1265..1546
                     /locus_tag="ACHKTW_005136"
                     /inference="COORDINATES: protein motif:HMM:NF022029.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
     gene            1577..1765
                     /locus_tag="ACHKTW_005137"
     CDS             1577..1765
                     /locus_tag="ACHKTW_005137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HFO6465988.1"
                     /translation="MATTPTLAHAKTAFVWRFIAFGVSGPQIINVTAWTEREARNRCP
                     SGCVAVFAARIRQGVSHD"
     gene            1758..1943
                     /locus_tag="ACHKTW_005138"
     CDS             1758..1943
                     /locus_tag="ACHKTW_005138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6465989.1"
                     /translation="MIKRFRVFSRAVNARGYTIGITQNVSATSVNNAIDKVKKDSHTA
                     GLTEVRIAHVLQLRNTA"
     gene            1957..2166
                     /locus_tag="ACHKTW_005139"
     CDS             1957..2166
                     /locus_tag="ACHKTW_005139"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6465990.1"
                     /translation="MAFKLIITLTNFDAGKVRKLISNNRYMTQEEAETAAKKMEYTYR
                     NGAGCITHTCAVDIQECNDRQGAKK"
     gene            2163..2405
                     /locus_tag="ACHKTW_005140"
     CDS             2163..2405
                     /locus_tag="ACHKTW_005140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6465991.1"
                     /translation="MKIENSKPAYWKTEEKTETVNMYQINASAWYDFRRVFICSGFDG
                     RTIEEAKEKARNYAANEGYYDFEIISVINFGAFDGK"
BASE COUNT          899 a          525 c          542 g          661 t
ORIGIN      
        1 caactaacaa caacttaatt aacagaattg acctgattat gaaagttacc gcacctttac
       61 gcaaaaaact cgacagcgca aaaaaagccc ttgaggtgct tgaaccagct cgcaaacaag
      121 tcgaggcttt aacttcatcg aataagaacg aaaaaaccaa atcaaattta tggtattttg
      181 aaaacgagat tctggaatac tctgaaccaa cagatcaaag ggttttacat tttttaataa
      241 ctaaactcaa tgaactgaag agtaacaaaa cagcagcaaa taaatttttt aaagattgca
      301 tttatatcgc attgaaaaga gagcgtgaaa gaagtattga tgatgaggca ttacatcgtt
      361 ttatttctac tggtgaggtt gaggcagttt attttctatt aaacagcaat atatcatccc
      421 gtgaatcaca aataataata tcttatttgt ttgtttatat ttgctttatt caaagctata
      481 ttgattatgt gaatgaaaaa acaaatatca ataatgatat ttgtattatc agaacaaaca
      541 gccattacca gaaactggca gttgatgcac aaaaaataat catcagagaa cttaacaaaa
      601 agcaaaccaa ctatagaata gctaagattc ttgaagatga attatcatca aaatacaaac
      661 atgttccaag taaaaacacg ttcaaaaact ggatcgaatt aataaaaaaa caccttaaac
      721 aaagaagcga caaataataa aaggatgtcc attcaaaata aagctcaaat attaccgact
      781 taaaaagtcg gtttttctgt tctctttaac ccagaaaaca cacctgagag caccggaacg
      841 acaggcaaaa agttttgggt tataaaccaa aaattatggc ctaaggttgg atcatttttg
      901 tgcttcgctt tcgatcgatt ctcttttacc gtcattaacg caatttaatg ccaatgacgc
      961 ttaaaggagg cttgttaatg gcggttaaga atgatttcag ccctgatcag cgcatgacgc
     1021 gtaaacaggc ggcggaatac ataggcgtat cagaaggaac gttaggtgta tgggcttgca
     1081 caggaaaggt aaaactaccg ttctacaagg ctggaagatg tcgcgttttt tacctaaaac
     1141 atgagcttga cgagtttttg cagtcaacaa ggcgctacca gtcataagcc aaaaaaaagc
     1201 cccgctgggc tactatcacg gggctaaaac aagctaataa gactttattg tataggcggg
     1261 gcagatgtta acagaaaaca aacggattaa aaagacgttg acaaacgcaa ataaaaaaca
     1321 tcagaatacc cccgaacctt ataaaacggg ttacgggatt ggaaccccgc aagcgatcaa
     1381 ggcgacacag acgccttcag cgtctttttt gttgtctttt tacatgcacg ccctatcaat
     1441 ggtgggctgt atggggccga cttcggtcgg gctggttccc ttgatcgcca gtagttccaa
     1501 ccccttacag tccaccgcca gcgagcttgg aacctccggc ggtggtaata atccactgat
     1561 cgaggaggct gcctttatgg ctactacccc aacccttgca cacgctaaaa ccgcctttgt
     1621 ctggcgcttt atcgcctttg gtgtatccgg ccctcaaatt atcaacgtaa ccgcctggac
     1681 ggaacgcgaa gcgcgtaacc gttgcccgtc tggttgcgtc gctgtatttg ccgcccgtat
     1741 ccgtcaggga gtgagccatg attaaaaggt ttcgtgtatt ttcccgtgct gtaaatgcgc
     1801 gtggttacac tatcggcatc acgcagaacg tgagcgcgac cagcgttaat aacgccattg
     1861 ataaagtaaa aaaagattca cacactgccg gactgacaga ggtcaggatc gctcacgtgt
     1921 tgcagctaag aaacactgca taaggagcta atcagcatgg cgtttaaact cattatcaca
     1981 ctgacaaact ttgacgccgg aaaagtcagg aagctgattt ctaataacag atacatgacc
     2041 caagaagagg cagaaacagc cgcaaaaaaa atggaataca catacaggaa cggcgcgggg
     2101 tgtattaccc atacttgcgc ggttgatatt caggaatgta acgacagaca gggggctaaa
     2161 aaatgaaaat cgaaaacagc aaaccagcat actggaaaac agaagaaaaa acggaaacgg
     2221 taaacatgta tcagattaac gcttctgcct ggtatgactt ccgtcgtgtg tttatctgtt
     2281 ctggttttga cggcaggaca attgaagaag caaaagaaaa agcacgtaac tacgcggcaa
     2341 atgaaggcta ttatgatttt gagattatca gcgtgattaa ttttggcgct tttgatggaa
     2401 aatagcgcac gtaatcctca atcaataaac agccacacaa tcggaattta cttttaatta
     2461 tggccttacg gtcaggggta tttatgcaag aaattaacag taataaaagc tatgaatgca
     2521 ataaagaatt tatttgcctg ttaaccgagg atgccgagga aagactacaa gaggttaaag
     2581 atattattta ccttttctgg aattgcattg attacgaaga tacagcg
//
LOCUS       DAZFQD010000266         2606 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.269, whole genome shotgun sequence.
ACCESSION   DAZFQD010000266 DAZFQD010000000
VERSION     DAZFQD010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2606
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.269"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..317)
                     /locus_tag="ACHKTW_005141"
     CDS             complement(<1..317)
                     /locus_tag="ACHKTW_005141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly chaperone"
                     /protein_id="HFO6465992.1"
                     /translation="MKKDLKTLALARLSGFRHKTVKVPEWGNVSVVLREPSAEAWYLW
                     QEVLNGDGEDDDTLSVVAKTRRNLEADVTLFCDVLCDTDLQRVFTPDDREQVLAVYGP
                     VHAR"
     gene            complement(332..1048)
                     /locus_tag="ACHKTW_005142"
     CDS             complement(332..1048)
                     /locus_tag="ACHKTW_005142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major tail subunit"
                     /protein_id="HFO6465993.1"
                     /translation="MSALYERSQLTQVMISSAPATAETMDKAEYLRLDCTIKEVQFTA
                     GQKQDIDVTTLCSTEQENINGLGASSEISMSGNFYLNQAQNALRDAYDNDALYAFKVL
                     FPSGKGFKFLAEVRQHTWSSGTNGVVAATFSLRLKGKPVSFVVPLAFVKNLDKTLTVN
                     TGALLTMSVSANGGTPPYKYAWKKDGQPVDGQTTDTFSKPGAQSADAGKYTCVVTDSA
                     EKAQSVTSVECTVTVSAAAG"
     gene            complement(1109..1453)
                     /locus_tag="ACHKTW_005143"
     CDS             complement(1109..1453)
                     /locus_tag="ACHKTW_005143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3168 domain-containing protein"
                     /protein_id="HFO6465994.1"
                     /translation="MTEADLYPHLAHLASGQVYPYVVPLLDGRPSVALPWVVFSLISS
                     VSADVMGGQAESSVSVQIDVYARTITEARQIRQAAREAVLVLAPESVSEMQDYIPENR
                     CHRATMEFQITV"
     gene            complement(1450..1896)
                     /locus_tag="ACHKTW_005144"
     CDS             complement(1450..1896)
                     /locus_tag="ACHKTW_005144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HFO6465995.1"
                     /translation="MIETLLDFSGLEDISRDLQLLSGAENNRVLREATRAGANVLKEE
                     VVSRAPVRRGKLRRNVVVLSRRSRDGGMESGVHIRGVNPDTGNSDNTMKADNPRNAFY
                     WRFVEMGTVNMPPHPFVRPAFDVRSEQAAQVAIARMNRAIDEVLRR"
     gene            complement(1893..2243)
                     /locus_tag="ACHKTW_005145"
     CDS             complement(1893..2243)
                     /locus_tag="ACHKTW_005145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFO6465996.1"
                     /translation="MAISAGRLTQMISVLNPVLTRNAAGEMTEEWVSCGKIHADIRGR
                     SSRERMQSGAEIAQAEIRIWVRGQSGREITAASRLHVLSGPWRDRILNVVGLPVPDAT
                     GGRLEILCRLGGEK"
     gene            complement(2253..2579)
                     /locus_tag="ACHKTW_005146"
     CDS             complement(2253..2579)
                     /locus_tag="ACHKTW_005146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFO6465997.1"
                     /translation="MTALLTLEEIKAHLRVEHDADDDMLMDKVRQATAVLLAYIQGSR
                     DKVIREDGELIPGEALTRMKGAAMRLTGMLYRNPDLAEREELIQGELPFSVSVLIYDL
                     RCPTVL"
BASE COUNT          572 a          867 c          611 g          556 t
ORIGIN      
        1 cgggcatgta ccggaccata gacggccagc acctgctcac ggtcgtccgg agtgaacacc
       61 cgttgcagat ccgtatcaca caggacatcg cagaacagcg tcacatccgc ttccaggtta
      121 cggcgggttt tcgccaccac cgacagggta tcgtcatcct ctccatcacc attgagcact
      181 tcctgccaca gataccaggc ctctgccgaa ggctcccgca gcaccacgct gacattaccc
      241 cattccggca ccttcaccgt tttatgacga aaccctgaca gtctggccag cgccagcgtt
      301 ttcagatcct ttttcatgat gacccatccc cttatccggc ggctgcgctc actgtcacgg
      361 tgcattcaac agacgtcaca ctctgtgctt tctctgccga atcggtcacc acgcaggtat
      421 atttccccgc atcagcggac tgcgcacctg gcttactgaa ggtgtctgtc gtctgcccgt
      481 caaccggctg accatccttc ttccaggcgt atttatacgg cggcgttccc ccgttggcac
      541 tgactgacat tgtcagcagc gcaccggtat tcacggtaag tgtcttatcc agatttttca
      601 caaacgccag cggtaccaca aaggacaccg gtttgccttt cagacgcagt gagaacgttg
      661 cagccaccac gccgttggta ccggatgacc aggtgtgctg acgcacttcc gccaggaatt
      721 taaagccctt accggacgga aacagcacct taaacgcata caacgcgtca ttgtcataag
      781 catcacgcag ggcgttctgg gcctgattca gataaaaatt acccgacatg gaaatctcgg
      841 aagacgcccc cagaccgttg atgttctcct gctctgtgga gcagagcgtg gtcacatcaa
      901 tatcctgttt ctgaccggcg gtgaactgga cttccttgat ggtgcagtcc aggcgcagat
      961 actccgcctt atccatggtt tcagccgtgg ccggagcaga tgaaatcatc acctgcgtca
     1021 gttgtgaacg ttcatacaaa gcagacattc tgcctcctga taacaaaaaa cccgcacgcg
     1081 gcgggtcagg gtttatgtag aaaaaagatc acaccgtgat ctgaaactcc atggttgcac
     1141 ggtgacagcg gttttccgga atatagtcct gcatttcact gactgattcc ggagccagta
     1201 ccagtacggc ctcacgggca gcctgacgta tctgacgggc ctccgtgatt gtcctagcat
     1261 aaacgtcaat ctgtaccgac accgatgact ccgcctgccc gcccatcacg tccgcagaca
     1321 ccgatgaaat caggctgaaa accacccacg gaagcgcaac cgacggcctg ccatccagca
     1381 gggggaccac atacgggtac acctgcccgc tggcaagatg cgccagatga ggatacaaat
     1441 ccgcctccgt catcgtctca gtacctcatc aatggcccgg ttcatccgcg caatcgccac
     1501 ctgcgctgcc tgttcactgc gcacatcaaa cgccgggcgc acaaacgggt gcggtggcat
     1561 attcacggtt cccatttcca caaaccgcca gtagaaagca ttgcgcgggt tatccgcctt
     1621 catggtgtta tcgctgttac cggtgtccgg attaacacca cggatatgga caccggactc
     1681 catcccgcca tcgcgggagc gccgggaaag gaccaccaca ttgcggcgca gttttcccct
     1741 gcgcaccggt gcccgtgaca ccacttcttc tttcagcaca ttcgcacccg cacgggttgc
     1801 ctcacgcagc acccggttat tttccgcacc actcagaagc tgcaaatcgc ggctgatgtc
     1861 ctccagcccc gaaaaatcca gcagggtttc gatcattttt cccctcccag ccgacagaga
     1921 atttccagac gcccgccggt cgcatccggc acgggcagcc cgacaacgtt caggatccgg
     1981 tcacgccatg gaccactcag cacatgaagt cgtgacgctg ccgtgatttc ccgaccggac
     2041 tgaccgcgca cccagatgcg gatttccgcc tgcgctattt ccgcaccaga ctgcatccgc
     2101 tcccggctgc tcctgccacg gatatccgca tgaattttcc cgcatgacac ccattcttcc
     2161 gtcatttctc cggcagcatt acgggttaac accgggttca gaacacttat catctgtgtc
     2221 agacgacctg cagatattgc cattccccct cctcataaca ccgtcggaca acgcaaatcg
     2281 taaatcaaca cggaaacaga aaacggcagc tccccctgaa tcagttcttc ccgctccgca
     2341 agatccggat tccggtacag catcccggtc agtcgcatgg cagccccctt catccgggtt
     2401 aatgcctcgc ccgggatcag ttcaccgtcc tcacgaatca ctttatcccg gctgccctga
     2461 atgtaggcca gcagcacggc ggtagcctga cgaaccttgt ccatcagcat gtcatcatca
     2521 gcgtcatgct cgacacgcag atgtgccttg atctcttcca gtgtcagtaa tgccgtcatt
     2581 ttccgcctcc agcatcccgc ccacgt
//
LOCUS       DAZFQD010000267         2589 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.270, whole genome shotgun sequence.
ACCESSION   DAZFQD010000267 DAZFQD010000000
VERSION     DAZFQD010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2589
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.270"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            237..431
                     /locus_tag="ACHKTW_005147"
     CDS             237..431
                     /locus_tag="ACHKTW_005147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001307652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFO6465998.1"
                     /translation="MSTKNRTRRTTTRNIRFPNQMIEQINIALEQKGSGNFSAWVIEA
                     CRRRLTSEKRAYTSIQSDDG"
     gene            792..1085
                     /gene="iss"
                     /locus_tag="ACHKTW_005148"
     CDS             792..1085
                     /gene="iss"
                     /locus_tag="ACHKTW_005148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="increased serum survival lipoprotein Iss"
                     /protein_id="HFO6465999.1"
                     /translation="MKKMLFSAALAMLITGCAQQTFTVGNKPTAVTPKETITHHFFVS
                     GIGQEKTVDAAKICGGAENVVKTETQQTFVNGFLGFITLGIYTPLEARVYCSQ"
     gene            complement(1176..1358)
                     /gene="rzoD"
                     /locus_tag="ACHKTW_005149"
     CDS             complement(1176..1358)
                     /gene="rzoD"
                     /locus_tag="ACHKTW_005149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage lysis lipoprotein RzoD"
                     /protein_id="HFO6466000.1"
                     /translation="MRKLKMMLCVMMLPLVVVGCTSKQSVSQCVKPPPPPAWIMQPPP
                     DWQTPLNGIISPSERG"
     gene            complement(1575..2072)
                     /gene="rrrD"
                     /locus_tag="ACHKTW_005150"
     CDS             complement(1575..2072)
                     /gene="rrrD"
                     /locus_tag="ACHKTW_005150"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme RrrD"
                     /protein_id="HFO6466001.1"
                     /translation="MPPSLRKAVAAAIGGGAIAIASVLITGPSGNDGLEGVSYIPYKD
                     IVGVWTVCHGHTGKDIMLGKTYTKAECKALLNKDLATVARQINPYIKVDIPETMRGAL
                     YSFVYNVGAGNFRTSTLLRKINQGDIKGACDQLRRWTYAGGKQWKGLMTRREIEREIC
                     LWGQQ"
     gene            complement(2072..2287)
                     /gene="essD"
                     /locus_tag="ACHKTW_005151"
     CDS             complement(2072..2287)
                     /gene="essD"
                     /locus_tag="ACHKTW_005151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage lysis protein EssD"
                     /protein_id="HFO6466002.1"
                     /translation="MKSMDKLTTGVAYGTSAGSAGYWFLQLLDKVTPSQWAAIGVLGS
                     LVFGLLTYLTNLYFKIKEDKRKAARGE"
BASE COUNT          736 a          630 c          558 g          665 t
ORIGIN      
        1 ttttcataaa tagcgaaaac ccgcgaggtc gccgccccgt aacctgtcgg atcgccggaa
       61 aggacccgca aaatgataat aattatcatc tacatgtcac aacgtgcatc tacgccatca
      121 aaccacgtca aataatcaat tatgacgcag gtatcgtatt aattgatctg catcaactta
      181 acgtaaaaac aacttcagac aatacaaatc agcgacactg aatacggggc aacctcatgt
      241 caacgaagaa cagaacccgc agaacaacaa cccgcaacat ccgctttcct aaccaaatga
      301 ttgaacaaat taacatcgct cttgagcaaa aagggtctgg gaatttctca gcctgggtca
      361 ttgaagcctg ccgtcggaga ctaacgtcag aaaagagagc atatacatca atccaaagtg
      421 atgatggatg aacatcccgg tttcttccac catcgcaccg gaaaagcgac tatgagggta
      481 accctgcgtc tgtcagcaca gtaaaacccg gtgtgcatcg tttttgatta ttcccgcaca
      541 ctcacgcaga aggaattccc cgtcgggcta cggtcatggt taatgcggga atacggcgac
      601 gatacagcgc agctaaaagg gtaatggaca gatagagcgg tttatttcat tccacaggat
      661 tctgagtgcc ccccctcctc caataggctg agcatccacc tatatagttt taattttcat
      721 caatccattt aactatcgtt taattgttgt cacataggat tctgccgttt ttaacaatgc
      781 aggataataa gatgaaaaaa atgttgtttt ctgccgctct ggcaatgctt attacaggat
      841 gtgctcaaca gacgtttact gttggaaaca aaccgacagc agtaacacca aaggaaacca
      901 tcacccatca tttcttcgtt tcgggaattg gacaggagaa aactgttgat gcagccaaaa
      961 tttgtggcgg cgcagaaaat gttgttaaaa cagaaaccca gcaaacattc gtaaatggat
     1021 ttctcggttt tattacttta ggcatttata ctccgctgga agcgcgtgtg tattgctcac
     1081 aataattgca tgagttgccc atcgatatgg gcagctctat ctgcactgct cattaatata
     1141 cttctgggtt ccttccagtt gtttttgcat agtgatcagc ctctctctga gggtgaaata
     1201 atcccgttca gcggtgtctg ccagtcgggg ggaggctgca ttatccacgc cggaggcggt
     1261 ggtggcttca cgcactgact gacagactgc tttgatgtgc aaccgacgac gaccagcggc
     1321 aacatcatca cgcagagcat cattttcagc tttcgcatca gctaactcct tcgtgtattt
     1381 tgcattgagc gcagcaacat cacgctgacg catctgcatg tcagtaattg ccgcgttcgc
     1441 cagcttcagt tctctggcat ttttgtcgcg ctgggctttg taggtaatgg cgttatcacg
     1501 gtaatgatta acagcccatg acaggcagac gatgatgcag ataaccagag cggagataat
     1561 cgcggttact ctgttcattg ctgaccccac aaacagattt cacgctcaat ctcacgacga
     1621 gtcatgagac ctttccattg cttaccgcca gcatatgtcc agcgacgtag ctgatcacat
     1681 gcgcctttga tatcgccctg gtttattttg cgaagaagcg tcgatgttct gaaattgcca
     1741 gcacccacgt tgtaaacgaa tgagtaaaga gcgccgcgca ttgtttccgg tatatcgact
     1801 ttgatgtacg ggttaatttg tctggcgacc gtggcaaggt ctttattcag gagggctttg
     1861 cattctgctt tggtatacgt tttaccgagc atgatgtctt ttcctgtatg cccgtgacat
     1921 acagtccata caccaacaat atctttgtat ggtatgtagc tgacaccttc cagaccatcg
     1981 ttaccacttg ggccagtgat taacactgat gctatagcaa ttgctccgcc accaatagca
     2041 gcagcaacgg cttttcgtaa tgatggaggc attattcacc tctcgcagcc ttgcgcttat
     2101 cttctttaat cttgaaataa aggtttgtca ggtacgtcag caggccaaat accaggctac
     2161 ccagcacacc tattgctgcc cactgtgagg gcgtgacttt atcgagcagc tgtaaaaacc
     2221 agtaaccggc actacctgct gaggtgccat aggcgacacc cgttgttaac ttatccatgg
     2281 atttcataac cccacctcgc agacaaagcg ggtgtaaatt gagggaatac aacgtatcgc
     2341 aaaaaagcag aaacgtaaca gactcggagt cagtgaataa ctcaggtatt gggttatcag
     2401 ctaatatcga gactcaaaaa atggaaaaac ccgctcgacg gcgggtttaa gctgtgtgac
     2461 gaagtaacca ctcttaacag cataaccaat tttttacgta cgtaaaccac aaaatgatat
     2521 ttgcgagaat gctaccgagt attgaaaaca ccactacaaa cacataagaa aatctcaaca
     2581 aataaccaa
//
LOCUS       DAZFQD010000268         2577 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.271, whole genome shotgun sequence.
ACCESSION   DAZFQD010000268 DAZFQD010000000
VERSION     DAZFQD010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2577
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.271"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..2193
                     /locus_tag="ACHKTW_005152"
     CDS             <1..2193
                     /locus_tag="ACHKTW_005152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HFO6466003.1"
                     /translation="PFLASPLIRDLARLSRKQESARREGLKNNSAYAADNFVRHGLRA
                     RLKRIARVNERFATPAFKATAARERLDELLILPQLSRDEIQRLATLTATAFSAALERA
                     CDEIIEKTGRGDDDLFTWLLTYQSLAHMALKLGITPPCWPSLEIRPDRRTAPNPERVP
                     GAVMRLSCATWWNSQLRHLRDIWREELLRAAGLVSRKTSVYISHEALLDWREKRQRTR
                     DFLKAWDIENEEGERFSLEDIYWAGLSNPRNRRNEMMTCARGMEQVAEARGDVAFFVT
                     VTAPSRFHSVNDDGSLNPKYCGATVRDASDYLVYRFFAAVRRAIDKAGLGWYGVRTVE
                     PHHDGTPHWHMLVFTSQENEARITEIMRNAAIREDRAELGDDISPRFKCEKIDPAKGT
                     PTSYIATYIGKNLDASAFMGNDPKTGKPYVDKESGKTMAETVENAIGWAGLHRIHQFQ
                     FFGIPPRQVWRELRSLAGQMARNPTAPQRLDHDDIDAILAAADVGCFATYITRQGGVL
                     IPRNTYLVRTAYETAEEANDYGEFPQRIYGVRAPLLGERYTICTHPDTWKLVRRKPEN
                     EDRTDEGFDVECGFAAPWTRGNNCPHDEKTDTGEGKTPPPETRKEQKLVVPGGDLGEW
                     LRSLTPTKRKQLTRQLKDATSDTKTVKTGSPTTPDDVIHVRTALPLTDVTTTVIKSAL
                     SATGEELHSGAALSVHRGARVRLPDGRIVHWDEKSRSLVAADQPEVNP"
     gene            2190..>2577
                     /locus_tag="ACHKTW_005153"
     CDS             2190..>2577
                     /locus_tag="ACHKTW_005153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6466004.1"
                     /translation="MTRFHCVLQRHDFSAIMSGRIPALTLSGVSVRPQPGDELEITIA
                     NPDRSTDTVMTDILTARINTDRSAFIVAVRPRRAISEPEPLTPVGKIMQAYHEKQTRK
                     LNEELARTLAVVNRKAAIVRYVHSAAS"
BASE COUNT          631 a          797 c          711 g          438 t
ORIGIN      
        1 ccgtttcttg ccagtccgct catccgggac ctggctcgcc tgagccgcaa acaggagagc
       61 gcacgccggg aagggcttaa gaataacagc gcatatgcgg cggataactt cgttcgtcat
      121 gggctgcgtg cccgcctgaa acgtatcgcc cgtgtgaatg aacgctttgc caccccggcc
      181 tttaaggcca ccgccgcccg cgaacggctg gatgaactgc ttatactgcc gcagctgagc
      241 cgtgacgaaa tccagcgcct tgccaccctg accgccaccg cgttttccgc cgcactggag
      301 cgcgcatgtg atgagatcat cgaaaagacc ggcaggggtg acgatgacct gttcacctgg
      361 ttactgacct accagtcact ggcacacatg gcgttaaaac tgggtatcac gccaccctgc
      421 tggccgtcgc tggaaatcag gccagaccgc cgcaccgccc ccaacccgga acgggtgccg
      481 ggtgcggtca tgcgcctttc ctgtgcgaca tggtggaaca gtcagttaag acacctccgg
      541 gacatctggc gtgaagagct gttacgcgcc gcaggtctgg tgtcacgtaa gacatcggta
      601 tatatcagcc atgaagcgtt actggactgg cgcgaaaaac gccagcgcac ccgcgatttt
      661 ctgaaggcct gggacatcga gaacgaagag ggagaacggt tcagccttga ggatatctac
      721 tgggcaggtc tctctaatcc gcgtaaccgc cgtaacgaga tgatgacctg cgcccgggga
      781 atggagcagg tcgccgaagc acgcggcgat gtggcgtttt tcgtcaccgt cacagcccct
      841 tcccgttttc acagcgtgaa cgatgacggc agtctgaacc cgaaatactg cggggcaacg
      901 gtacgcgatg ccagtgatta tctggtttac cgctttttcg ccgccgtccg cagggccatc
      961 gacaaggccg ggctgggctg gtacggcgtg cgcacagtgg agccccacca cgacggtaca
     1021 ccgcactggc acatgctggt attcaccagc caggaaaacg aagcccgtat cactgaaatc
     1081 atgcgcaacg ccgccatcag ggaggaccgc gcagagctgg gtgatgatat ctccccgcgt
     1141 tttaaatgcg aaaaaatcga cccggcaaaa ggcacaccga caagctatat cgcgacctat
     1201 atcggtaaga acctggacgc cagcgccttc atgggaaatg acccgaaaac cggcaaacct
     1261 tacgtcgata aggaatcagg caaaaccatg gccgaaaccg tggagaacgc catcggctgg
     1321 gcaggccttc accgcatcca ccagttccag ttcttcggta tcccgccccg ccaggtatgg
     1381 cgcgaactcc gcagccttgc cgggcagatg gcccgtaacc cgactgcccc acagcgtctg
     1441 gaccatgacg acattgacgc catactggcc gccgccgacg tgggatgttt tgccacctat
     1501 atcacccgcc agggtggtgt gctcattccc cgcaacacgt acctggtccg caccgcatac
     1561 gaaactgccg aagaggccaa cgactacggc gaattcccgc aacgcattta cggcgtccgc
     1621 gccccgttac tcggggagcg ctacaccata tgcacccacc cggacacctg gaaactggtc
     1681 agacgtaagc cggaaaacga agaccgcacg gacgagggtt ttgacgttga gtgcggcttt
     1741 gccgcacctt ggactcgtgg caataactgt ccccatgatg aaaaaacaga caccggagag
     1801 ggtaaaaccc caccaccgga aaccaggaaa gaacaaaaac tggtcgtccc gggaggtgat
     1861 ttgggtgagt ggttaaggtc actgaccccc acaaagcgaa aacaactcac caggcagctg
     1921 aaagatgcga catctgacac aaaaaccgtg aaaaccggca gccccaccac gcccgatgac
     1981 gtcatacatg tgcgtacagc attgccgctg actgacgtca ccaccaccgt aatcaagtcg
     2041 gcactgagcg cgacgggcga ggagctacac agtggcgcag cactttccgt acacagaggt
     2101 gcacgcgttc gcctgcctga cgggcggatt gtgcactggg atgagaaatc acgaagcctg
     2161 gtggcagcag atcaaccgga ggtgaaccca tgacacgatt ccattgtgta ctccagcgcc
     2221 atgatttcag cgccatcatg agtggacgca ttccggcact gacgctgagc ggcgtcagtg
     2281 ttcgaccaca gccaggcgac gaactggaga tcacgatcgc aaaccccgat cgcagcacag
     2341 ataccgtcat gaccgacatc ctgactgcaa gaatcaacac cgacagaagc gcattcatcg
     2401 ttgccgtgcg ccctcgtcgc gccatatcag agcctgaacc actgactccc gtaggaaaaa
     2461 tcatgcaggc ataccacgaa aaacagacca gaaaactcaa cgaagaactg gcaagaacgc
     2521 tggccgtcgt taaccgaaaa gcggcaattg tccgctatgt gcattcggcc gccagca
//
LOCUS       DAZFQD010000269         2518 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.272, whole genome shotgun sequence.
ACCESSION   DAZFQD010000269 DAZFQD010000000
VERSION     DAZFQD010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2518
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.272"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..2193
                     /locus_tag="ACHKTW_005154"
     CDS             <1..2193
                     /locus_tag="ACHKTW_005154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HFO6466005.1"
                     /translation="SFLGNVLIRDLARLNRKQQSARNEGVKNNNTHVADNFVRRGLRT
                     RLKRIEQVNECFATTAFKATAARHRLDELLMLPQLNRDQIQRLATLTAAAFSTELERI
                     CDEVTERTGKGDDNLFTWLLTYQQLARMAIKLGVNPPYWPSLEIRRDRRTAPDPERVP
                     GAVMRITCATWWNNQLRHLADLWREELLRAAGRVSRKASPYISHESLQEFREKRQRTR
                     DFLKSWDIENEDGERLSLEDVYWSGLGNPRNRRNEMMACVRGMEQVAESRGDSAFFVT
                     VTCPSRFHSVNEDGSLNQKYNGATVRDASDYLVYDVFAAARKKLNKEGLSWYGVRTVE
                     PHHDGTPHWHMLVFTAPDNEERIIEIMRDAAIKSDRAELGNDISPRFKCEKIDPAKGT
                     PASYIATYIGKNLDASTFHDNDPKTGKPYADKESGKTMAETVENAIAWASLHRIRQFQ
                     FFGIPPRQVWRELRRLAGQMERNPASPKRLDHDDIDAILAAADVGCFATYINRQGGVL
                     IPRNLYLIRTAYETADEANDYGEFPQRIYGICAPSLGERYTICTHPDEWKLVRKEAKA
                     DNSTDEDFDVGGGFAAPWTRGNNCPHDEKTDTGEGKTPPPETRKEQKLTIPEGDLSEW
                     LRSLTPTERKQLTRQLKDAPSDTKTVKTGGPTTPDDVIHVRTALPLTDVTTTVIKSAL
                     STTGEELHSGAALSVHRGARVRLPDGRIVHWDEKSRSLVAADQPEVNP"
     gene            2190..>2518
                     /locus_tag="ACHKTW_005155"
     CDS             2190..>2518
                     /locus_tag="ACHKTW_005155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6466006.1"
                     /translation="MTRFHCVLQRHDFSTIMNARIPALTLSGVSVRPQPGDELEITIA
                     SPDRSTDTVMTDILTARINTDRSAFIVAVRPRRAISEPEPLTPVGKIMQAYHEKQTRK
                     LNEELART"
BASE COUNT          681 a          761 c          656 g          420 t
ORIGIN      
        1 tcgtttcttg gcaacgtgct cattcgtgat ctggcgcgtt taaaccgcaa acaacaaagc
       61 gcccgcaatg aaggcgtgaa aaacaacaac actcacgtgg ccgataactt cgttcgccgt
      121 ggactgcgca cacgcctgaa acgcatcgaa caggtgaatg aatgcttcgc cacgacggca
      181 ttcaaagcca ccgcagcgcg tcatcgcctt gatgaattac tcatgctgcc acaactcaac
      241 cgtgatcaaa ttcagcgcct tgccacacta acggcggcgg cattttccac cgaactggaa
      301 cgcatatgtg atgaagttac tgagcggaca ggaaaaggcg acgataacct gtttacctgg
      361 ttgctgactt accagcagct ggcgcgcatg gcaataaaac tgggcgtgaa cccaccatac
      421 tggccgtcac tcgagatcag acgtgaccgc cgcacggcac cagaccctga acgggtaccg
      481 ggcgcggtaa tgcgtatcac ctgcgccacc tggtggaaca accagctgcg ccacctcgct
      541 gatctctggc gggaggaatt actgcgtgcc gccggaagag tatcgcgcaa ggcatccccc
      601 tacatcagcc atgagtcact ccaggaattc cgggagaaac gccagcgcac ccgtgatttt
      661 ctcaaaagct gggacattga aaacgaagac ggtgaacgcc tgagcctgga ggacgtgtac
      721 tggtcagggc tggggaatcc gcgcaaccgc cgcaacgaga tgatggcctg cgtcagaggg
      781 atggaacagg tcgccgaatc gcgtggtgac tccgcgttct tcgtgacagt gacctgccct
      841 tcccgctttc acagcgtaaa cgaggacggc agcctgaacc agaaatacaa cggtgccacc
      901 gtgcgcgatg ccagtgatta tctggtttac gacgtctttg cagctgcaag aaaaaaactc
      961 aacaaagaag gcctgagctg gtacggggtg cgcaccgttg agccacacca tgacggcaca
     1021 ccgcactggc acatgctggt attcaccgcc ccggacaatg aagaacgcat cattgaaatc
     1081 atgcgtgacg cagcaatcaa atcagatcgc gcagaactgg ggaatgatat ttctccgcgc
     1141 ttcaagtgcg aaaaaatcga cccggcaaaa ggcacaccgg caagctacat cgcaacctat
     1201 attggcaaga acctggacgc cagcaccttt cacgacaatg acccgaaaac cggcaaacct
     1261 tacgccgata aagaatcggg aaaaaccatg gcggaaaccg tggaaaacgc cattgcctgg
     1321 gcgagtctcc accgcatccg gcagttccag ttcttcggta tcccgccccg ccaggtatgg
     1381 cgtgaactcc gccgccttgc cggacaaatg gagcgtaacc ccgcatcacc gaagcgcctg
     1441 gaccatgacg acattgacgc cattctggcc gccgctgacg tggggtgttt tgccacctac
     1501 atcaaccgtc agggcggcgt gctcattcca cgcaacctgt acctgatccg caccgcatac
     1561 gaaaccgcag atgaggccaa cgactacggc gaattcccgc agcgcatcta cggaatttgt
     1621 gccccgtcgc tcggcgagcg ctacaccatc tgcacccatc cggacgagtg gaaactggtc
     1681 agaaaagaag cgaaagccga caacagcacg gacgaggatt ttgacgttgg gggcggcttc
     1741 gccgcacctt ggactcgtgg caataactgt ccccatgatg aaaaaacaga caccggagag
     1801 ggtaaaaccc caccaccgga aaccaggaaa gaacaaaaac tgaccatccc ggaaggtgat
     1861 ttgagcgaat ggttaaggtc actgaccccc acagagagaa aacaactcac caggcagctg
     1921 aaagatgcgc catctgacac aaaaaccgtg aaaaccggcg gccccaccac gcccgatgac
     1981 gtcatacatg tgcgtacagc attgccgctg actgacgtca ccaccaccgt aatcaagtcg
     2041 gcactgagca cgacgggcga ggagctacac agtggcgcag cactttccgt acacagaggt
     2101 gcacgcgttc gcctgcctga cgggcggatt gtgcactggg atgaaaaatc acgaagcctg
     2161 gtggcagcag atcaaccgga ggttaaccca tgacacgatt ccattgcgta ctccagcgcc
     2221 atgatttcag caccatcatg aacgcacgca ttccggcact gacactgagc ggcgtcagtg
     2281 ttcgaccgca gccaggcgac gaactggaaa tcacgatcgc cagtcccgat cgcagcacag
     2341 acaccgttat gaccgacatc ctgactgcaa gaatcaacac cgacagaagc gcattcatcg
     2401 ttgccgtgcg ccctcgtcgc gccatatcag agcctgaacc actgactccc gtaggaaaaa
     2461 tcatgcaggc ataccacgaa aaacagacca gaaaactcaa cgaagaactg gcaagaac
//
LOCUS       DAZFQD010000270         2495 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.273, whole genome shotgun sequence.
ACCESSION   DAZFQD010000270 DAZFQD010000000
VERSION     DAZFQD010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2495
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.273"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            55..864
                     /gene="yqeI"
                     /locus_tag="ACHKTW_005156"
     CDS             55..864
                     /gene="yqeI"
                     /locus_tag="ACHKTW_005156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO6466007.1"
                     /translation="MYWIINDNIEFWPEHRKLISVHNADLNVVLTTPASRCLSLLLEA
                     FPDVVAQQDFFTRVWEEEGMRVPTNTLYQNISIIRRGFRAVGDTTHSLIATVPRRGFK
                     IHNDINIQNHVINSSTDAHTHNAPPAIKVNAGYKESIGGAKNFNNKILKHIKSHLIML
                     SAFVIGAYSAYWLWNNNQPKPFFKDYKTVAEINGCHFNVTEDTIDGLKEFDKYKTRIL
                     DSGINCKKHPWLYFPLAKSSPGMIVMACNKNYNQHEVADCLTLSYREVNRD"
     gene            857..1339
                     /gene="yqeJ"
                     /locus_tag="ACHKTW_005157"
     CDS             857..1339
                     /gene="yqeJ"
                     /locus_tag="ACHKTW_005157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqeJ"
                     /protein_id="HFO6466008.1"
                     /translation="MIDYKKNLLFILVFISGFILFTVYSYTAEKMIYNETCTANWVIF
                     NDQGRANLTIDFMFNKKNKTGTVALSGTWQQGNRESKSIRRNIEYTWIENYDTAHLTS
                     KKVNKFEIMDQVDDDRLVQLIPDFYVFPEKSVSYNILKQGKHAFILSIGNRAIMHCAR
                     "
     gene            complement(1372..1452)
                     /locus_tag="ACHKTW_005158"
     CDS             complement(1372..1452)
                     /locus_tag="ACHKTW_005158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6466009.1"
                     /translation="MKDVDQNFDALDCHILREYLILLFYD"
     gene            complement(1488..1912)
                     /gene="yqeK"
                     /locus_tag="ACHKTW_005159"
                     /pseudo
     CDS             complement(1488..1912)
                     /gene="yqeK"
                     /locus_tag="ACHKTW_005159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417326.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqeK"
     gene            2106..>2495
                     /locus_tag="ACHKTW_005160"
     CDS             2106..>2495
                     /locus_tag="ACHKTW_005160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6466010.1"
                     /translation="MKPRNINNSLSLQPLVPDQENKNKKNEEKSVNPVKITMGSGLNY
                     IEQESLGGKYLIHDLSIKIADISEEIIQQAILSAMSIYKFSITDDLMSMAVNELIKLT
                     KIENNVDLNKFTTICTDVLSPLVTRHNK"
BASE COUNT          885 a          438 c          421 g          751 t
ORIGIN      
        1 atacttagca aactatgtga tctccatttc gattgattta gtgtttattg acgtatgtac
       61 tggattatta acgataatat cgagttctgg cctgagcacc gaaaattaat atcggtacat
      121 aacgccgatc ttaacgtcgt tctgacaacg ccagccagtc gatgtttatc acttctactt
      181 gaagcttttc ctgatgtggt tgcacaacaa gattttttca ccagagtctg ggaagaagaa
      241 ggtatgcgtg tgcctactaa cacgttatat cagaacatat ccattatcag acgcggattt
      301 cgcgctgttg gtgatactac ccactcgcta attgcaaccg tgccgagaag aggattcaag
      361 atccataatg acattaacat acaaaatcat gtaataaact cgtcaacaga cgcacataca
      421 cacaatgccc cacctgccat aaaagttaat gcgggataca aagagagcat tggtggcgca
      481 aagaatttca ataacaaaat cctcaaacat ataaaaagtc atctaattat gttgagcgca
      541 tttgtcatag gcgcatattc tgcatattgg ctatggaata ataatcaacc gaagccattt
      601 ttcaaggatt acaagactgt tgcggaaatt aatggctgcc attttaatgt aacagaagat
      661 acaattgacg ggttgaaaga gttcgataaa tataaaacac ggatactgga ttcggggatt
      721 aactgtaaaa aacatccgtg gttatacttc cctcttgcaa aatcctcacc ggggatgatt
      781 gttatggcat gcaataaaaa ttataaccaa catgaagtgg cagactgttt aaccctgtct
      841 taccgtgagg ttaatcgtga ttgattataa aaaaaactta ttatttattt tagtttttat
      901 cagtgggttt attctcttca ctgtctattc ttacactgca gaaaaaatga tatataacga
      961 aacatgcact gcaaattggg taatttttaa tgaccaggga cgagcaaatc ttaccatcga
     1021 ctttatgttt aacaaaaaaa ataaaacagg aactgttgcg ttgagtggaa cctggcaaca
     1081 aggtaacaga gaaagtaaat caatacggcg aaatattgaa tacacatgga ttgaaaacta
     1141 tgacacagcc catttaacat caaagaaagt taataagttt gagattatgg atcaagttga
     1201 tgatgataga cttgtacaac ttattcctga tttttatgtc tttccagaaa aaagtgtaag
     1261 ctataatata ctaaagcaag gtaagcatgc ttttattttg agcattggta acagagcaat
     1321 aatgcattgt gcaaggtaaa ctaaaataga taacaaaaag atggaacctc gttaatcata
     1381 aaacaataaa attaaatatt ctcgcagtat atggcagtct aaagcatcaa agttttgatc
     1441 aacatctttc attttagaca tctccttgca atgtaaaata tcatgtatca acaacatctg
     1501 gtgcaaatcc atgagtcgaa ctcgacattc atcttagctc gattattatt ccttcgtaca
     1561 gtcgatgtaa acaatacaga aagaggatta ttaagaacca gtttactcag tgaatcaata
     1621 gaggaaagga ttaacgtttc tttaaaagaa ttaatttcat catctgttaa actaaactca
     1681 tcattgacag atcgtgagat ataactgttt ttaactttac tctttacgtt gactttattg
     1741 acagaattaa cattcacata tcttgaattt aatgtccatg atgttgtttg cgtgagaacc
     1801 ttctcagcat taatgaattt tttaacggaa tccttggttt ctttttcgag tccgaattta
     1861 caatatcatg catatagacc atttcatgaa tctgcgaaaa ctcaatgtcc attgtatacc
     1921 tcacattttt accgtgactc gatgttactg ttcaataatc accttccatc aatactaaaa
     1981 ttaatacccc taatgtgccg ataacaaata tagtcattct acgtaacgtc tccataaggt
     2041 gatatttgac attatcagaa gctgcgaatt cggattttgc tctaatcaaa tgagagaaat
     2101 gtaaaatgaa gccacgaaat attaataata gcctatcact gcaaccatta gttcctgatc
     2161 aggagaacaa aaataagaaa aatgaagaga aatccgttaa tccagttaaa atcacaatgg
     2221 ggtctggttt aaattatatt gaacaagaat ctcttggagg aaaatatcta atacatgatt
     2281 tgtcaataaa gatagcggat atttctgaag agataattca gcaagcaata ttatctgcta
     2341 tgagcatata taaattttcg ataacagatg atttaatgag tatggctgta aatgaactca
     2401 taaaactgac caaaatagag aataatgtag acctgaataa attcactact atatgcacag
     2461 acgttctatc ccccctcgtc accagacata ataaa
//
LOCUS       DAZFQD010000271         2495 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.274, whole genome shotgun sequence.
ACCESSION   DAZFQD010000271 DAZFQD010000000
VERSION     DAZFQD010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2495
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.274"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..952
                     /locus_tag="ACHKTW_005161"
     CDS             <1..952
                     /locus_tag="ACHKTW_005161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="multidrug DMT transporter permease"
                     /protein_id="HFO6466011.1"
                     /translation="EQKSGSAMNQLFNTLSDGWTVISDGLHDVQAMTDTISDKIDALE
                     NAVSGMGIVQDVSAFTASFTAIKGNAAALINAPRRMAESLAGIFAVLTGLPGNPSLSL
                     TGKAGRPSGSLTTNRDSMTEQAMPQLYRTLSSLRYTLSEQDDPQRLIGLTPAAQKNIR
                     LLRSVMQSATLVSQAQTVGKLLDQVIRQNTTHVQDTDRAGGLAWLESSADVQRINRDL
                     SDAMEQQVLDLSAQGHTGTALALRDAGLALTEDLTTRSVHLPGAFQVMVRTTEPALVT
                     LYRATGNSRRWQYFVRRNNIPDPVFVPGGRSVEVISEQQD"
     gene            936..2060
                     /locus_tag="ACHKTW_005162"
     CDS             936..2060
                     /locus_tag="ACHKTW_005162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein"
                     /protein_id="HFO6466012.1"
                     /translation="MSSRIELYIGGSIFSGWLTVSVRRSLEHLAGSFELGLMLPGERI
                     PSALRTGQSLTLRINGQTVISGWLDQVSQRISATRHQISISGRDKTGDLVDCAAIHPG
                     SQWRNRTLAQIAADLCAPFGIAVRWQVNDDTAARPFSSFTLENSETVADALTRSARHR
                     GVLVTSNADGDLVFTQAGSQQTDRLVLGDNLLDADYNTDWRGRYSEYRVRGHGRGGGK
                     RGDSESAARLAAPVGVISDEQIGRYRPKIILADQQTDTTGARQRALREMRRAIARSER
                     FSATVRGWFRDDGRLWDVNLLTGVSAQRFGIEQTELLVCQVEFLLDEQNGEVTRLVLA
                     PRDGFIVPAEPDSKGRGGSGDDVDAFIRQQMKKQGISFDE"
     gene            2050..>2495
                     /locus_tag="ACHKTW_005163"
     CDS             2050..>2495
                     /locus_tag="ACHKTW_005163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HFO6466013.1"
                     /translation="MMNDEVFSRLIAPVTRGIRLLFGRGVLTGTHDELKMQNVQLTGM
                     DGETFDDVERPQQYGQISVPLPGAETFFACLGGQRDQTVVLVVEDRRSRPTGLKAGDT
                     GVYHHEGHRIRLTKDGRIIVTCKMLEIYADEGMRVDTPEATFTGNVT"
BASE COUNT          521 a          679 c          791 g          504 t
ORIGIN      
        1 ggagcagaaa agcggttcag caatgaatca gctgtttaac acgctttcag acggctggac
       61 ggtgatttca gacggtctgc atgatgttca ggccatgacc gacaccatca gcgataagat
      121 tgatgcgctg gagaatgccg tttcagggat ggggattgtg caggatgtca gtgcctttac
      181 ggccagtttt accgccatta aaggcaatgc cgccgccctg attaatgcgc cccgtcgcat
      241 ggctgaatcg ctggccggaa tatttgccgt gctgaccggg ctgcccggca atccttccct
      301 gtcactgacc gggaaggcag gaaggccgtc cggcagcctg accacaaacc gtgacagcat
      361 gacagagcag gcaatgccgc agctgtaccg cacactttct tccctgcgtt acaccctcag
      421 cgaacaggat gatccgcagc gcctgattgg tctgacgcct gcggcacaga aaaatatccg
      481 tctgctgcgg agcgtcatgc agagtgcaac cctggtgtcg caggcgcaga cggtcgggaa
      541 actgctggat caggtcatcc ggcagaatac aacgcacgtt caggatactg accgtgcggg
      601 tggactggcc tggctggaaa gttctgccga tgtgcagcgt atcaaccggg atttgagtga
      661 tgccatggaa caacaggtac tggatctgtc cgcacagggc cataccggca ccgcccttgc
      721 attacgtgat gcagggcttg cgctgacaga agatttaacc acgcggagtg ttcatcttcc
      781 cggcgctttt caggtcatgg tgcgaaccac cgaaccggca ctggtcacgc tgtaccgtgc
      841 caccggcaac agccgccgct ggcagtattt tgtccgcaga aacaacatcc ccgatccggt
      901 ttttgttccg ggtggccgca gcgtggaggt gatcagtgag cagcaggatt gaactgtata
      961 tcggcggcag tattttttcc ggctggctga cggtcagcgt ccgtcgctcg ctggagcatc
     1021 tggccgggtc gtttgaactg gggctgatgc tgcccggtga gcgcatcccc tcagccctgc
     1081 ggaccggtca gtccctgacg ctcagaatta acgggcagac ggtcatcagt ggctggctgg
     1141 atcaggtcag tcagcggatc agtgcaaccc gtcatcagat cagcatcagc ggccgggata
     1201 aaaccggcga tctggtggac tgtgccgcca tccaccccgg cagtcagtgg cgcaaccgga
     1261 cgctggcaca gattgccgca gatttatgtg cgcccttcgg gatagctgtg cgctggcagg
     1321 ttaacgacga cacggctgca cgccctttca gctcttttac gctggaaaac tcagaaaccg
     1381 tggcggatgc gctgacccgt tctgcccggc atcgcggggt gctggtgacc agtaatgctg
     1441 acggcgatct ggtgttcacc caggcaggaa gccagcagac ggacagactg gtgctgggag
     1501 ataacctgct tgatgccgat tacaacacgg actggcgagg acgatacagt gaataccgtg
     1561 tccggggaca cgggcgcggt ggtggcaaac ggggagacag cgagtccgcc gcccggctgg
     1621 cagcacctgt gggcgtcatc agtgatgagc agatcggccg ctaccggccg aaaatcatcc
     1681 tcgccgatca acagacagac accaccggtg cacggcagcg tgccctgcgt gaaatgcgcc
     1741 gtgcgattgc ccgttcagaa cggttttctg ccaccgtgcg tggctggttc cgggatgatg
     1801 gccggttatg ggatgtcaat ctgctgaccg gtgtttcggc ccagcgtttc ggtatagaac
     1861 agactgaact gctggtctgt caggtggagt ttttactgga tgaacagaac ggggaagtca
     1921 cccggctggt actggcaccg cgtgacggct ttatcgttcc ggcagagccg gacagtaagg
     1981 gccggggtgg ttccggtgac gatgttgatg cctttattcg ccagcagatg aaaaaacagg
     2041 ggatcagctt tgatgaatga tgaagtgttc agccgcctga ttgccccggt aacgcgcggt
     2101 attcgcctgc tgtttggccg gggcgttctg accggcacac atgacgaact gaaaatgcag
     2161 aatgtgcagc tcaccggcat ggacggcgaa acctttgatg atgtggagcg cccccagcag
     2221 tacgggcaga tcagcgttcc cctgccgggt gcagaaacct tttttgcctg tctgggcgga
     2281 cagcgggatc agactgtggt gcttgtggtg gaagaccggc gcagccgtcc gaccggactc
     2341 aaagccggag atacgggggt gtatcaccat gaggggcacc ggatacggtt aacaaaggat
     2401 ggtcgcatta ttgtgacatg caaaatgctg gagatttacg ccgacgaggg gatgcgggtg
     2461 gatacgccgg aagccacctt tacgggcaat gtgac
//
LOCUS       DAZFQD010000272         2489 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.275, whole genome shotgun sequence.
ACCESSION   DAZFQD010000272 DAZFQD010000000
VERSION     DAZFQD010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2489
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.275"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            126..635
                     /gene="eco"
                     /locus_tag="ACHKTW_005164"
     CDS             126..635
                     /gene="eco"
                     /locus_tag="ACHKTW_005164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004867 - serine-type endopeptidase
                     inhibitor activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HFO6466014.1"
                     /translation="MKKESKKMKTILPAVLFAAFATTSAWAAESVQPLEKIAPYPQAE
                     KGMKRQVIQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLENKTLEGWGYDYYVFD
                     KVSSPVSTMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAE
                     EKIDNAVVR"
     gene            complement(784..2430)
                     /gene="mqo"
                     /locus_tag="ACHKTW_005165"
     CDS             complement(784..2430)
                     /gene="mqo"
                     /locus_tag="ACHKTW_005165"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008924 - L-malate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HFO6466015.1"
                     /translation="MKKVTAMLFSMAVGLNAVSMAAKAKASEEQETDVLLIGGGIMSA
                     TLGTYLRELEPEWSMTMVERLEGVAQESSNGWNNAGTGHSALMELNYTPQNADGSISI
                     EKAVAINEAFQISRQFWAHQVERGVLRTPRSFINTVPHMSFVWGEDNVNFLRARYAAL
                     QQSSLFRGMRYSEDHAQIKEWAPLVMEGRDPQQKVAATRTEIGTDVNYGEITRQLIAS
                     LQKKSNFSLQLSSEVRALKRNDDNTWTVTVADLKNGTAQNIRAKFVFIGAGGAALKLL
                     QESGIPEAKDYAGFPVGGQFLVSENPDVVNHHLAKVYGKASVGAPPMSVPHIDTRVLD
                     GKRVVLFGPFATFSTKFLKNGSLWDLMSSTTTSNVMPMMHVGLDNFDLVKYLVSQVML
                     SEEDRFEALKEYYPQAKKEDWRLWQAGQRVQIIKRDAEKGGVLRLGTEVVSDQQGTIA
                     ALLGASPGASTAAPIMLDLLEKVFGDRVSSPQWQATLKAIVPSYGRKLNGDVAATERE
                     LQYTSEVLGLKYDKPQAADSTPKPQLKPQPVQKEVADIAL"
BASE COUNT          598 a          673 c          616 g          602 t
ORIGIN      
        1 attagaattt aacactaaaa gagcaggtaa aattgtctga atgttcttta agttattcat
       61 aaagcaaatt aataaatctg atgaatatgt taaccttcag cgacatcatc ggtgaaaacc
      121 tataaatgaa gaaggaaagc aaaaaaatga agaccattct acctgcagta ttgtttgccg
      181 ctttcgctac cacttccgcc tgggcggcag aaagcgtcca gccactggaa aaaatcgcgc
      241 cttatccaca agctgaaaaa gggatgaagc gtcaggtgat tcagttaacc ccgcaagaag
      301 atgaatctac cctgaaagta gaactgttaa tcggtcagac gctggaagtc gattgcaatt
      361 tgcatcgtct cggcgggaag ctggaaaaca aaacgctgga aggctggggc tatgattatt
      421 atgtctttga taaagtcagt tccccggttt cgacgatgat ggcctgcccg gatggcaaga
      481 aagagaagaa atttgtcacc gcgtatctgg gcgatgctgg aatgctgcgt tacaacagca
      541 agctgccgat cgtggtgtat acgccagaca atgtagatgt gaagtaccgc gtctggaagg
      601 cggaagagaa aattgacaac gcggtagttc gctaaaatgc tgtgaagtgc ggcaccccgt
      661 aggccgaata aggcggtcac gccgcatccg acatctaacg cccgagccgg tgcctgatgc
      721 gacgctggcg cgtcttatca gcatacgcca catccggcat accatgccgg atgtggcaca
      781 tcattacaac gcaatatccg ccacttcttt ttgaacgggt tgcggtttca actgcggttt
      841 tggcgtacta tctgctgcct gcggcttgtc gtatttcagc cccagcactt cgctggtgta
      901 ctgcaactcg cgttctgttg ccgctacatc accgttcagc ttgcgtccat aagacggaac
      961 gatcgctttc aacgtagcct gccattgcgg gctggaaacg cgatcgccaa atactttttc
     1021 cagcagatcc aacataatcg gcgcggcggt tgacgcccct ggcgatgccc ccagcagcgc
     1081 ggcaatggtc ccttgctggt cactgaccac ttcagtaccc agacgcagta cgccaccttt
     1141 ctcggcatca cgcttgataa tctgcacgcg ctgccccgct tgccacaaac gccagtcctc
     1201 tttttttgcc tgcggatagt actctttcaa cgcttcaaaa cgatcctctt cactcaacat
     1261 cacctgactc accagatatt tcaccagatc gaaattatcc agcccgacgt gcatcatcgg
     1321 catcacgtta gaggtggtgg tggaactcat cagatcccac aatgaaccgt ttttgaggaa
     1381 tttggttgag aaggtggcaa atggcccaaa cagcactacg cgtttaccgt ccagaacgcg
     1441 ggtatcgata tgcggaaccg acatcggtgg tgcgccaacg gatgctttac cgtaaacctt
     1501 cgccagatgg tgattaacca cgtccgggtt ttccgaaaca aggaactgtc cgcccaccgg
     1561 gaaaccggcg tagtctttcg cttccggaat ccccgattcc tgtaatagct tcagcgccgc
     1621 accgcccgcg ccgataaaga caaatttggc gcggatgttc tgtgcagtgc cgtttttcag
     1681 atcggcaacg gtaacggtcc aggtgttatc gtcattacgc tttagggcgc ggacttcgct
     1741 gctgagttgc agcgagaagt tagatttctt ctgtaaggaa gcaattaact ggcgggtgat
     1801 ctcgccatag ttcacatcag tgccaatttc tgtgcgtgtt gccgctactt tctgttgtgg
     1861 atcgcgccct tccatcacta acggtgccca ctctttgatc tgcgcgtgat cttcagagta
     1921 acgcataccg cgaaacagcg agctttgttg caacgcggcg taacgggcgc gcaggaaatt
     1981 aacgttatcc tcgccccaga caaagctcat atgcggaacg gtattgataa atgaacgcgg
     2041 agtacgcagc acgccgcgct caacctggtg cgcccagaac tggcgggaaa tctgaaatgc
     2101 ttcgttaatg gcgactgctt tttcaatact gatgctgcca tcggcgtttt gcggggtgta
     2161 gttcagttcc atcagtgcag aatgcccggt tccggcgtta ttccagccgt tcgaactctc
     2221 ctgcgcgaca ccctccaggc gctccaccat ggtcatcgac cattcaggct ccagctcgcg
     2281 taaataggtc cccaacgtgg cgctcataat gccgccgcca atcaacagta catcagtttc
     2341 ctgctcctcg gacgctttcg cttttgccgc catcgaaacg gcattaagcc ccacggccat
     2401 cgagaagagc atggcagtca cttttttcat cttgttaatg ccttactttt agtcgcttta
     2461 ttgcaggtga gatttgcgcg gcatcaacg
//
LOCUS       DAZFQD010000273         2479 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.276, whole genome shotgun sequence.
ACCESSION   DAZFQD010000273 DAZFQD010000000
VERSION     DAZFQD010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2479
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.276"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..882)
                     /locus_tag="ACHKTW_005166"
     CDS             complement(<1..882)
                     /locus_tag="ACHKTW_005166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000218374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFO6466016.1"
                     /translation="MTVRISGVLKDGTGKPVPGCTIELKARRTTETVIVTTVAQGQPG
                     ETGSYSFDVEPGWYRVTLNTEGYAPSYVGDILVKADSEPGTLNKFLMEQDEAQYYPKA
                     LAELEAVAAEILKRAEASAASAEEAKKRAENARGPAGEKGDTGPQGATGAQGPAGATG
                     AVGPKGEPGPKGERGETGPQGPKGDKGDPGGPPGPKGDTGPRGEAGPPGPQGPAGQTG
                     PKGDKGEPGATGPAGPAGSVASVPDASTSQKGVVQLSSDTNSDDETKAATPKAVKAVM
                     AEVQAAKTKAEEAATRAA"
     gene            complement(1067..>2479)
                     /locus_tag="ACHKTW_005167"
     CDS             complement(1067..>2479)
                     /locus_tag="ACHKTW_005167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HFO6466017.1"
                     /translation="FRINAPAAPAGVELTPGYFQITAVPRLAVYDPTVQFEFWFSEAK
                     IADAAQVETSARYLGTGSQWSVSGSQIKPGKVFWFYVRSVNLVGKSAFVEASGRASDD
                     AAGYLEFFREKIGKTHLAEALWAEIDNSKLKDEMAEMQTTITETRNEITQTVSKTLED
                     QNATIQQIQRVQTDTNNDLNSMWAVKLQQMQDGRRYIAGIGAGIENTPDGMQSQILLA
                     ADRVAFINPENGDTTPALVTQGGQTFINEALIKVLTAPTITSGGNPPAFSLTPDGRLT
                     ARNADISGNISASSGTLNNVTIAEDCTINGTMSADRIIGDIVKGYTVDMGQGVMTQNQ
                     LTFQKMILLKDNMPFDRKLMLMAYSFSRGEFIMKINDKVVVHARSGKVKTGEREVQIS
                     KNASRYEPVYSDVGGSASGSWSIPAGQGDVRVEITFRYEADVLFYMGGSILHDKSKIR
                     IHDPVLFYLKSYTDDFDTAS"
BASE COUNT          455 a          785 c          586 g          653 t
ORIGIN      
        1 tgctgcccgg gttgctgcct cttctgcctt cgtttttgct gcctgcactt ctgccattac
       61 cgctttcaca gcctttgggg ttgccgcttt tgtttcatca tcgctgttgg tatcgctgct
      121 cagctgtaca acacctttct gtgatgtgct ggcatccgga acactggcga cactgcctgc
      181 gggacctgcc ggacctgttg cgccgggttc acctttatcc cctttcgggc cggtttgccc
      241 tgccggaccc tgtggtccgg gtggtccggc ctctccacgt gggccggtgt cacctttcgg
      301 ccccggcggt ccgcccgggt cacctttatc gcccttcggt ccctgtggac ctgtttctcc
      361 ccgttctccc tttggccccg gctcaccttt tggtccgacc gcccccgttg ccccggctgg
      421 tccctgtgcc cctgtggcac cctgtggacc tgtgtccccc ttctcgcccg ccggtccccg
      481 ggcattctct gcccgtttct tcgcttcctc tgcactcgcc gccgacgctt cagcacgctt
      541 caggatttcc gctgccaccg cttccagctc tgcaagcgct ttcgggtaat actgcgcctc
      601 atcctgttcc atcagaaatt tattcagcgt tcccggctca gaatccgcct tcaccagaat
      661 gtcacccaca tacgacgggg cgtacccttc cgtgttcagc gtcacccggt accaccccgg
      721 ctccacatca aaactgtaac tgccggtttc ccccggctgc ccctgcgcca ccgtggtgac
      781 tatcactgtc tctgtggtgc gtcgcgcttt cagctctatg gtgcatcccg gcaccggctt
      841 cccggtgccg tccttcagca cgcccgatat tctgactgtc atagatttct cccataaaaa
      901 aaaccgcagt accggttacc cggtctgcga tcaggggtat aaattgtgac tggctggtgt
      961 taaagggtgc cgtcagtcaa aggcacccgt ggataatgta cgatgtccgg cttttgtcgt
     1021 tttccggcaa tcacgaacag atggatgaaa cctcactatc cacctgtcac gatgcggtat
     1081 caaaatcgtc tgtataggat ttcaggtaaa acagaaccgg gtcatggatc cgaattttac
     1141 tcttatcatg aagtattgaa ccacccatgt aaaaaagcac atctgcttca tacctgaatg
     1201 taatttcaac ccgcacatca ccctgtccgg caggaatact ccatgagcct gacgcacttc
     1261 cgccaacatc agaatatacc ggctcatatc tgctggcgtt tttggaaatc tggacctccc
     1321 gctccccggt tttgactttg cctgacctgg catgaacgac aactttgtca tttatcttca
     1381 tgatgaattc acctctggaa aatgaatacg ccataagcat aagcttgcga tcaaacggca
     1441 tgttgtcttt cagcaaaatc attttctgaa aagtcagctg gttctgagtc attacgccct
     1501 gccccatatc aaccgtgtat cccttgacga tatcaccaat gattcggtca gcgctcatcg
     1561 ttccattaat ggtgcagtct tccgcaatcg tcacgttatt cagcgtaccg gaactggcac
     1621 tgatattccc gctgatatcg gcattacggg ccgtcagcct gccgtccggc gtcagggaga
     1681 atgccggggg attgccgccg ctggtaatgg tgggggcggt aagaaccttt atcagggcct
     1741 cattgataaa cgtctgtccc ccctgcgtga ccagtgcggg tgtggtatca ccattctccg
     1801 gattaatgaa tgccacacgg tccgctgcca gcagtatctg gctctgcatc ccgtcagggg
     1861 tgttctcaat accggcaccg ataccggcaa tatagcgacg accatcctgc atctgctgta
     1921 gcttcaccgc ccacatgctg ttcaggtcat tatttgtgtc cgtctgcaca cgctggattt
     1981 gctgtatggt ggcgttctgg tcctccagcg ttttactgac cgtctgcgtg atttcattgc
     2041 gggtttctgt gatggtggtc tgcatttccg ccatctcgtc cttcagcttg ctgttgtcaa
     2101 tctctgccca caacgcctct gccagatgtg tttttcctat cttttcccgg aaaaactcca
     2161 gatagcccgc agcatcatca ctggcccgtc cactggcttc aacaaacgcg gatttaccca
     2221 ccagattgac gctgcgcacg taaaaccaga aaactttccc gggtttaatc tggctgccgg
     2281 agacgctcca ctggctgcct gtccccagat aacgggcaga ggtttccacc tgtgcggcgt
     2341 ctgcaatttt tgcctccgaa aaccagaact caaactgcac cgtcgggtca tacaccgcaa
     2401 gacgcggtac cgccgttatc tgaaaatacc ccggcgtcag ttcaacaccg gcaggcgctg
     2461 ccggtgcgtt aatccggaa
//
LOCUS       DAZFQD010000274         2464 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.277, whole genome shotgun sequence.
ACCESSION   DAZFQD010000274 DAZFQD010000000
VERSION     DAZFQD010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2464
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.277"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            176..739
                     /locus_tag="ACHKTW_005168"
     CDS             176..739
                     /locus_tag="ACHKTW_005168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFO6466018.1"
                     /translation="MLTTQKRKFALALMSGKNKTASAIAAGYSAKTARVKGSQLAKDP
                     EVLAFIARKQCETVEVDEVPVYRQKKSEQEDKPRRREAAAIPQPDETNPEMPPPVVIS
                     PGIEYMEDGLPDPVKAMGRLLVENINTDPRLALDAAYKLAQFTHHKKGDAGKKSAKGD
                     AAKKAANRFAVPPPPRLVVNNDNEGNG"
     gene            736..2397
                     /locus_tag="ACHKTW_005169"
     CDS             736..2397
                     /locus_tag="ACHKTW_005169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HFO6466019.1"
                     /translation="MIPVWSTACPDWAERLKKGLSIIPSPIYPEQAAHALAIFKQLRI
                     VDAPGSPTFGESCAAWVFDLVAALFGSYDAQTGVRHIKEVFILIPKKNSKSTLAAGIM
                     MTALLLNWRQAAGYTILAPTVEVAANAFNPARDMVRRDDDLDDLCQVQTHIRTITHRV
                     TDTTLKVVAADPNTVSGIKSVGTLIDELWLFGKQYKAEDMLREAIGGLASRPEGFVVY
                     TTTQSNEPPAGVFRQKLQYARDVRDGKIHDPHFLPVIFEHPPEMVESGAHLLMENLAM
                     VNPNLGYSVDEAFLYREYRKAREAGEEAFRGFMSKHANVEIGLALRSDRWAGADFWEQ
                     QGRRVSLDDILQRADVVTVGIDGGGLDDLLGMYVIGRDRETREWLGWGHAWAHETAVL
                     RRKSEASRFQDFVACGDMTIVRRVGDDTAEVAEYVRRIHEAELLDHIGIDPSGVGQIL
                     DSLAEAGIPDGIVVGISQGWKLGGAIKTTERKLAEGVLVHGGQPLMAWCVGNARVETK
                     GNAILITKQASGRGKIDPLMALFNAVSLMSLNPGPKKKEYAVFFI"
BASE COUNT          557 a          593 c          792 g          522 t
ORIGIN      
        1 ccgggcattt ttgcggtttt tatctttatt attcagtttg ttgcgtggaa aaaatgttaa
       61 caggcttttt cagcaaatgt taaccaggca gcagttaaca tttgcggcat gagacgccgg
      121 gaaaaatggg ctgaaccata cccggctgag tgcgttctgg acccgggagg aggctgtgct
      181 gacaacgcaa aaacgaaaat ttgcgctggc gctcatgtcc gggaaaaaca aaacagcgtc
      241 agccattgcc gccggttatt cggcgaagac agccagggtt aaaggctcgc agctggcaaa
      301 agatccggag gtgcttgcgt ttatagcccg taaacagtgc gagacggtgg aggtggatga
      361 ggttcctgtt taccggcaga aaaaatcaga gcaggaggat aaaccccgtc gccgtgaggc
      421 ggctgcaata ccacagccgg acgaaacaaa tccggagatg ccaccgcccg tggtgatatc
      481 tcctggtatt gagtatatgg aggacggtct tcccgatcct gtgaaagcga tggggcgtct
      541 tctggtggag aacattaata ccgaccccag gctggcgctg gatgcggctt ataagctggc
      601 gcagttcacg caccacaaaa aaggggatgc cggtaaaaaa tcggcaaaag gtgacgcggc
      661 gaaaaaagcg gctaaccgtt ttgcggtgcc accaccaccc cgcctggtgg tgaataatga
      721 taatgagggc aacggatgat accagtgtgg agcacggcct gcccggactg ggcagagcgt
      781 ctgaaaaagg ggctgtcgat tattccgtct ccgatttacc cggagcaggc cgcacatgcc
      841 ctggcgattt ttaaacaact gcggattgtg gatgcaccgg gcagcccgac gttcggggag
      901 tcctgtgcag cgtgggtgtt tgacctggtg gcggccctgt ttggctccta cgatgcgcag
      961 accggtgtac gccatataaa ggaagtgttc attctgatac cgaaaaaaaa cagcaagtcc
     1021 acgctggccg cggggatcat gatgacggcg ctgttactga actggcggca ggcggcgggc
     1081 tacaccattc tggccccgac cgtggaggtg gcggctaacg ccttcaaccc tgccagggat
     1141 atggttcgac gggacgatga tctggatgac ctctgtcagg tgcagacaca tatccggacc
     1201 atcacccatc gggtgacgga caccaccctt aaggtggtgg ctgccgatcc gaatacggtg
     1261 tccggtatca agtccgtggg gacgctgatt gatgaactgt ggttatttgg caagcagtac
     1321 aaagcggagg acatgttacg tgaagccata ggcggccttg cctcccgccc ggaagggttt
     1381 gtggtgtata cgaccaccca gtcgaatgag ccgccagccg gagtgttcag acagaaactg
     1441 cagtacgccc gggatgtccg tgacggcaaa attcatgatc cgcactttct gccggtgata
     1501 tttgaacacc ctcctgaaat ggtggaaagc ggggctcacc tgctgatgga aaacctcgcc
     1561 atggtcaatc cgaatctcgg ttattcggtg gatgaggctt ttctgtaccg ggagtaccgt
     1621 aaagcccggg aggctggtga ggaagcattt cgtggcttca tgtcaaaaca cgccaatgtg
     1681 gaaatcggtc ttgccctgcg ttctgaccgc tgggcgggcg cggatttctg ggagcagcag
     1741 ggcaggcgcg tcagcctgga cgatatcctg cagcgcgctg atgtggtgac ggtggggatt
     1801 gacggcgggg gcctggatga tctgctggga atgtacgtga ttggccgtga cagggaaacc
     1861 cgcgaatggc tgggctgggg ccatgcctgg gcgcatgaaa ccgcggtgct cagacggaag
     1921 agtgaggcat cccggtttca ggattttgtg gcctgtggag acatgacgat tgtccgtcgg
     1981 gtcggggatg acacggcgga agtggcggag tatgtgcgtc gtatccatga ggctgagtta
     2041 ctggatcata tcggtattga cccgtcaggt gtggggcaga ttctggattc actggcggaa
     2101 gccgggatcc ccgacggaat tgttgtgggg ataagccagg gctggaagct gggcggggcc
     2161 atcaaaacca ccgagcgcaa actggctgag ggagtgctgg tgcatggtgg tcagccactg
     2221 atggcctggt gcgttggcaa tgcccgggtg gagactaaag gtaacgccat ccttattacc
     2281 aaacaggcca gcggacgggg gaaaattgac ccgctgatgg cgctgttcaa tgcggtatcc
     2341 ctgatgtccc tgaatccggg gccgaaaaag aaagaatatg cggttttttt catataaccc
     2401 tgttcaccct gtaaccatca tggaccgctg cggcggtttt tttattttca ggaggctgat
     2461 gtga
//
LOCUS       DAZFQD010000275         2459 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.278, whole genome shotgun sequence.
ACCESSION   DAZFQD010000275 DAZFQD010000000
VERSION     DAZFQD010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2459
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.278"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..894)
                     /locus_tag="ACHKTW_005170"
     CDS             complement(<1..894)
                     /locus_tag="ACHKTW_005170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HFO6466020.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLAMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAA"
     gene            1230..2432
                     /gene="nupC"
                     /locus_tag="ACHKTW_005171"
     CDS             1230..2432
                     /gene="nupC"
                     /locus_tag="ACHKTW_005171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HFO6466021.1"
                     /translation="MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
BASE COUNT          616 a          631 c          570 g          642 t
ORIGIN      
        1 ggcagcaacc agacttaacc caaagaccgt tgccgcagca tggcttaaca gcggttgcag
       61 cgtcagataa gcctcatcaa gatcggcaac accagtatga ccagaaaagt ggaacgccgc
      121 cgcagctgta gccatcatcg ccagattgac aaaaccggca atcgtcatgg cgatagccac
      181 atcccatttg gtggcggaat aacgttgttg acgcgaaccg ccatgtaaat gctgagtgag
      241 cgaagagtgc aaataaatca catgcggcat aatcgtcgcc cctaacacgc ctgctgccag
      301 gaagaccgct tccgaagtag gtaaactcgg gatcaccatt cctttaccca gctgcgccag
      361 gttaggctgg gaaaaaatca actcgacaat gtaagccgcg gcaacaaaca acagtaaccc
      421 accaatcact ttttccagcg gtttttgccc gcgacgttgc agcattaaaa tcaggaaagt
      481 cgcgatcccc gtcagcaccg cgccctgcaa caacgagaca ccaagaatga gtttaaaacc
      541 gatcgccgca ccaataaatt ccgccaggtc ggttgccatc gcaataattt ctgcctgaac
      601 ccaatagaac cacactacgg gacgcggata gtgatcgcga atctgctccg ccagattttt
      661 accggtggca atccctagtt tggcagagag gatctgaatc agcatcgcca tcaggttggc
      721 ccaaacgaca acccacagta gctgatagcc gaagctggca cccgcctgaa tattggtcgc
      781 aaagttaccg ggatcgatat aaccaatcgc cgcaatgaac gcaggtccca ttaatgcgag
      841 cctcatcttg cgcgccgccc gtccgctgct actctcaacg cgatagttcg tcatcttgtg
      901 cctctaaaac atagcctttg ctatgtttca tgctatgcca aacgagaatg attatcaaat
      961 tcatttaaat gggttgtggt gatttctctg atagacctgg attatgacta cgaaaagatt
     1021 gatgacttga atgttatggt tatgtttaat gttagcacat ttacataact ttcagcttcc
     1081 atacacaaca tagcagaaat gtatgacaga tcactatttt tgaagcttgt cacaggacgt
     1141 cattatagtg tgtgtcagat ctcgttttcc ttaaccatgt tacatagaat gtgcacggaa
     1201 atttaacctg cctcatattt ggagcaaata tggaccgcgt ccttcatttt gtactggcac
     1261 ttgccgttgt tgcgattctc gcactgctgg taagcagcga ccgcaaaaaa attcgtatcc
     1321 gttatgttat tcaactgctt gttatcgaag tgttactggc gtggttcttc ctgaactccg
     1381 acgttggttt aggcttcgtg aaaggcttct ccgaaatgtt cgaaaaactg ctcggatttg
     1441 ccaacgaagg gactaacttc gtctttggta gcatgaatga tcaaggcctg gcattcttct
     1501 tcctgaaagt gctgtgccca atcgtcttta tctctgcact gatcggtatt ctccagcaca
     1561 ttcgcgtgtt gccggtgatt atccgcgcaa ttggtttcct gctctccaaa gtcaacggca
     1621 tgggcaaact ggaatccttt aacgccgtca gctccctgat tctgggtcag tctgaaaact
     1681 ttattgccta taaagatatc ctcggcaaaa tctcccgcaa tcgtatgtac accatggcag
     1741 caacggcgat gtccaccgta tctatgtcca tcgttggtgc atacatgacc atgctggagc
     1801 cgaaatatgt cgttgcggcg ctggtactga acatgttcag cacctttatc gtgctgtcgc
     1861 tgatcaaccc ttaccgtgtt gatgccagtg aagaaaacat tcagatgtcc aacctgcacg
     1921 aaggtcagag cttcttcgaa atgctgggtg aatacattct ggcaggtttc aaggttgcca
     1981 ttatcgttgc cgcgatgctg atcggcttta tcgccctgat cgccgcactg aacgcactgt
     2041 ttgctactgt gactggctgg tttggctaca gcatctcctt ccagggcatc ctgggctaca
     2101 tcttctatcc gattgcatgg gtgatgggtg ttccttccag tgaagcactg caagtgggca
     2161 gtatcatggc gaccaaactg gtttccaacg agttcgttgc gatgatggat ctgcagaaaa
     2221 ttgcttccac gctctctccg cgtgctgaag gcatcatctc tgtgttcctg gtttccttcg
     2281 ctaacttctc ttcaatcggg attatcgcag gtgcagttaa aggcctgaat gaagagcaag
     2341 gtaacgtggt ttctcgcttc ggtctgaaac tggtttacgg ctctaccctg gtgagtgtgc
     2401 tgtctgcgtc aatcgcagca ctggtgctgt aaaaccatac ataaaaaagc cggggatca
//
LOCUS       DAZFQD010000276         2422 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.281, whole genome shotgun sequence.
ACCESSION   DAZFQD010000276 DAZFQD010000000
VERSION     DAZFQD010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2422
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.281"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..263
                     /gene="hdeB"
                     /locus_tag="ACHKTW_005172"
     CDS             <1..263
                     /gene="hdeB"
                     /locus_tag="ACHKTW_005172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HFO6466022.1"
                     /translation="NAQSALAANESAKDMTCQEFIDLNPKAMTPVAWWMLHEETVYKG
                     GDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQASNDLPN"
     gene            327..974
                     /locus_tag="ACHKTW_005173"
     CDS             327..974
                     /locus_tag="ACHKTW_005173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HFO6466023.1"
                     /translation="MTAEFIIRLILAAIACGAIGMERQMRGKGAGLRTHVLIGMGSAL
                     FMIVSKYGFADVLSLDHVGLDPSRIAAQVVTGVGFIGAGNILVRNQNIVGLTTAADIW
                     VTTAIGMVIGSGMYELGIYGSVMTLLVLEVFHQLTFRLMNKNYHLQLTLVNGNTVSML
                     DWFKQQKIKTDLVSLQENEDHEVVAIDIQLHATTSIEDLLRLLKGMAGVKGVSIS"
     gene            complement(1016..1546)
                     /locus_tag="ACHKTW_005174"
     CDS             complement(1016..1546)
                     /locus_tag="ACHKTW_005174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HFO6466024.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIIFSPFNIKRCLGKVPVNFVPRTITIIDFVALI
                     NGSYCSVPEAAVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(1702..2268)
                     /gene="slp"
                     /locus_tag="ACHKTW_005175"
     CDS             complement(1702..2268)
                     /gene="slp"
                     /locus_tag="ACHKTW_005175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HFO6466025.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
BASE COUNT          697 a          485 c          537 g          703 t
ORIGIN      
        1 tgaacgcaca atctgcgctg gcagccaatg aatccgctaa agatatgacc tgccaggaat
       61 ttattgatct gaatccaaaa gcaatgaccc cggttgcatg gtggatgctg catgaagaaa
      121 cagtatataa aggtggcgat accgttactt taaatgaaac cgatctcact caaattccta
      181 aagtgatcga atactgtaag aaaaacccgc agaaaaattt gtataccttc aaaaatcaag
      241 catctaatga cttgccgaat taatgaggta caagtaaaaa ggagtagcaa gttgagccat
      301 cttgctgctc ctttttgcat ttttatatga cagcagaatt tattatacgt cttatactcg
      361 cggcaattgc ctgtggcgct attggcatgg aaaggcaaat gcgcggcaaa ggagcagggt
      421 tacgcacaca tgtattaatt ggcatgggaa gcgccctgtt tatgattgtt tcgaaatatg
      481 gttttgctga cgtgctgtct ttagatcacg tcggactcga ccccagccgt attgctgctc
      541 aggtggtgac gggcgtcggg tttatcggtg caggtaacat tctcgttcgt aaccaaaata
      601 ttgtaggtct gacgacggca gcggatatct gggtgaccac cgccataggt atggttattg
      661 gcagcggtat gtacgaactg ggtatttatg gttcagtgat gaccttgttg gtgctggaag
      721 tcttccatca attaaccttc cgcctgatga acaaaaatta ccatttacag ctgacattag
      781 tgaacggtaa tacggtatca atgctcgact ggtttaagca gcaaaaaata aaaacggatt
      841 tagtttcgtt gcaggaaaat gaagatcatg aagtggtcgc cattgatatc cagcttcatg
      901 ccacaacgtc gatagaagac ctgttgcgat tattaaaagg tatggcaggg gtgaaaggcg
      961 tttcaattag ctaatctgtg tggcagcgta gccagactca ccgtaagcct gaaattcaca
     1021 ccagataatc aatatgctga tggcgtacca gctcattgat ccgcttaaga ttgaggatca
     1081 tcatgatatt gtgtttatgg cagtagaacg tttttagcga tattttcagt ttctccagaa
     1141 tatcttccgt tgtcatttga ttcgcaatgc agctcagaac ctgatgttgc ttgcgcgaaa
     1201 gtgacacagc cgcttcaggc acagagcagt aactgccatt gatgagtgcg acaaaatcaa
     1261 tgatagtgat agtccgcgga acaaagttca ccggcacttt ccccaggcag cgtttaatat
     1321 taaaggggga gaaaataatc acatggacag gttttaatcg ttcaatttga gtgagaaaat
     1381 tagaatgaaa tacattattc attaatgtat caataatgac gaaggcattc tgatgcaaca
     1441 ttacgtcgat ctcttcttcg ttctgtatat gaacgacatt acctttactc agaatgtttt
     1501 tcatcgcggt gaagaacatc gtatccctgg taattataag aaacataacc gactcctggt
     1561 gcggacatta actttataat cagtttgcca aaggtaatta acaggacagg gcttataata
     1621 ataatagtta gcttactgta tattaatttt atgtaataca tgatgcagat gctgagaaag
     1681 catctgcatc tttcggtggt gttatttgac cagctcaggt gttacctgac tcaccgcatt
     1741 ggtgtagtaa ggcgcacccc agcccggttc cggccagaat gcaccatagc cgtaatccca
     1801 caggttatag gtggtattaa ccacttctct caaatgccac acctggatgc cctgcatatt
     1861 cacttccagg aagttatacg ggactttatt gataaagcct ggttgttcac cctgaatggt
     1921 accgaggatg gtaacaaagt gattacgata gttcactgga tcaaggaagc cgctttgtct
     1981 ggcgagcagt cggccctgat agttggcttc aatatcaggc ttcgcatagc tatccaacgg
     2041 taatacagcg atttctaaca acgtatccgt tttgccattg ataacgttga taaccttccc
     2101 accaaagcgc gcttgttgac caacatataa ccccggctgg ttatgaacag caacaaaact
     2161 tttttgaata tcaggttggt tattgccttt gatattttgc ggaattgaac tacatgcggc
     2221 aagcaaaaat gaaaggctga ggatgagtgc accttttgtc atgttcatgt tactatcctt
     2281 atcaacaaat attaaaacca taatagatat ttctttatag ttttcatctg attctgaggt
     2341 gatatgcctg atagtaacgt aaacctgtct gatgtttata tcccttctgt ttagttcaat
     2401 tccgttattt tggggcgtgg ta
//
LOCUS       DAZFQD010000277         2389 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.282, whole genome shotgun sequence.
ACCESSION   DAZFQD010000277 DAZFQD010000000
VERSION     DAZFQD010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2389
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.282"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..560
                     /locus_tag="ACHKTW_005176"
     CDS             <1..560
                     /locus_tag="ACHKTW_005176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PrgH/EprH family type III secretion apparatus
                     protein"
                     /protein_id="HFO6466026.1"
                     /translation="VWVKQALEKNQPGKPVRVINPDDESIRIFSWLADNFPDLQYFKL
                     QLLDASNLRLTVSKQRNAITQQLIDNLIKGLLQTMPYASNISIAVVDDNVLESQAIET
                     LSAIGLSYEKYKTANNVYFNIIGTLSDSELNKINNYVDEYYKQWGKQYVRFNVNLKNQ
                     DTNNSSFSYGDNRFEKSQGSNWTFQ"
     gene            577..817
                     /locus_tag="ACHKTW_005177"
                     /pseudo
     CDS             577..817
                     /locus_tag="ACHKTW_005177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311745.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle complex
                     protein"
     gene            837..1168
                     /gene="sctI"
                     /locus_tag="ACHKTW_005178"
                     /pseudo
     CDS             837..1168
                     /gene="sctI"
                     /locus_tag="ACHKTW_005178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311744.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner rod subunit
                     SctI"
     gene            1165..1899
                     /locus_tag="ACHKTW_005179"
                     /pseudo
     CDS             1165..1899
                     /locus_tag="ACHKTW_005179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311743.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EscJ/YscJ/HrcJ family type III secretion inner
                     membrane ring protein"
     gene            1915..>2389
                     /locus_tag="ACHKTW_005180"
     CDS             1915..>2389
                     /locus_tag="ACHKTW_005180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030257 - type III protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion apparatus protein OrgA/MxiK"
                     /protein_id="HFO6466027.1"
                     /translation="MNLALRKIIYDPISYIHPQRVSLNNTPINNPVLRSITNEMIVLQ
                     YNLSVEHFNLNSSLIYYINNWNLFPLFCLFSGYHFYRERFAERGFFYKVPAVLRDYLS
                     AIPVKINEKARYKPGIASYHNIITCGFSTLSPYIRQQPLAMQQRFNLLFPDFVDHI"
BASE COUNT          806 a          398 c          455 g          730 t
ORIGIN      
        1 cagtctgggt caaacaagca ctggaaaaaa atcaaccagg aaagccagta cgggtgataa
       61 atcctgatga tgaatcgata cgaatttttt catggcttgc tgataatttc cctgatttac
      121 aatattttaa acttcaacta ttagatgcca gtaatctaag attgacagtg agtaagcaac
      181 ggaacgctat cacacagcaa ctaatcgata atcttattaa aggattactg caaactatgc
      241 catatgccag caatataagt attgcggtag tagatgataa tgtattggaa agtcaggcta
      301 ttgaaacgtt gtcagcgata ggtctttctt atgaaaaata taaaacagct aacaatgtgt
      361 atttcaatat cattggtacg ttaagtgaca gtgaattaaa taaaattaat aactatgttg
      421 acgaatatta taaacaatgg ggtaaacaat atgtaagatt taatgtgaat ttgaaaaatc
      481 aggacacaaa taatagttca tttagctacg gagataaccg attcgagaag tctcaaggta
      541 gcaactggac atttcagtaa taattgagga ggacttatgg cagattggaa tggttatatt
      601 atggatatca gcaatcaatt tgatcagggc gttgatgatc tgaaccagca agttgaaaaa
      661 gcgttggagg atttagccaa ccaatccctc cgacacgaaa tttcttgccg aatatcaaag
      721 tgcattagct gagtatacat tatatcgaaa tgcgcaatct aacgttgtaa aagcgtataa
      781 agatcttgat tcagcaatca tacaaaactt ccgctaaact ggataaggat tttattatgt
      841 cagtaagcaa tatgccacct attgatagag ctgagcaatc aactgctcat gaaatacagc
      901 aagcgaaagt gattgattta aatgaccggg ttctcaatct tgacaacccg gatgataaaa
      961 tgatctccgc ttttgccaac tatgctgtac aaactgaaaa ttggcagcaa aatgcgttac
     1021 aagcattaag gtctgataaa gaggggttaa ctcctgaaaa attattagtc ttgcaggatc
     1081 atgtgcttaa ttataatgtc gaggtttcat tggtggaaca ttagcaagaa agattgtcgc
     1141 tgcagttgag acgttgaccc gctcatgaaa tatatatcat tattattgtt tatcctgctg
     1201 ttgtgcggct gcaagcagca ggaattattg aaccatcttg atcaacagca agctaatgat
     1261 gtccttgctg ttttacaaag acataatatt aatgctgaaa agaaggatta aggcaaaaca
     1321 ggattttcaa tatttgtcga accaacagat tttgcttctg cggtggattg gctaaaaatc
     1381 tacaatctac cgggtaagcc tgatattcaa atatcgcaga tgttccctgc agatgcactc
     1441 gtttcatctc cacgagcgga aaaagcaagg ctatattctg ctattgagca gcggttagag
     1501 caatctctca aaattatgga tggcattatt tcttcgagag tacatgttag ttacgatgtt
     1561 gataatggcg atagtggtaa aactgcttta ccaattcata tttctgtact tgcagtatat
     1621 gaaaaagata tcaacccaga aataaaaata aatgatatca agagatttat tgttaatagc
     1681 tctgcaagcg tacaatatga aaatatttct gttgtattgt caaaacgaag agacattatt
     1741 gagcaagcac caacatatga aattagcgaa cctgtttttg cttatgataa ggctatgccg
     1801 gtttctatct tacttgcatt aatatccgta gcgacttgct ggctgttatg gaagtatagg
     1861 gccattctaa ctaatttagt caggttgaaa attaagtaga catatgagag cgttatgaat
     1921 cttgccctaa gaaagattat atatgatcca attagttata ttcatcctca acgagtaagc
     1981 ttgaataata cacctattaa caatccggtg cttagatcta ttaccaatga aatgattgtt
     2041 ctgcagtaca atctttcagt tgaacatttt aatctgaata gttcacttat ttactacata
     2101 aacaattgga atttatttcc attgttctgt ttattcagtg gttatcactt ttatcgagaa
     2161 aggtttgcag aacgcggatt tttttataag gttcctgctg tgctaaggga ttatttatca
     2221 gctataccgg tgaaaataaa tgaaaaggca cgatacaaac ccggcatcgc tagctatcac
     2281 aatatcatca catgtggttt ttcaacgttg tcaccctata taaggcaaca accattggca
     2341 atgcagcaac gatttaattt attatttcct gattttgttg atcacatac
//
LOCUS       DAZFQD010000278         2344 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.283, whole genome shotgun sequence.
ACCESSION   DAZFQD010000278 DAZFQD010000000
VERSION     DAZFQD010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2344
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.283"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(82..1494)
                     /gene="pykF"
                     /locus_tag="ACHKTW_005181"
     CDS             complement(82..1494)
                     /gene="pykF"
                     /locus_tag="ACHKTW_005181"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HFO6466028.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEQGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
     gene            complement(1502..1696)
                     /locus_tag="ACHKTW_005182"
     CDS             complement(1502..1696)
                     /locus_tag="ACHKTW_005182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6466029.1"
                     /translation="MDCRTQRLKNRHSRWAETQAHIPLHAFSMSPILRARHHHFRNTG
                     FAFRQCAQKASFSHPSQLKD"
     gene            2051..2260
                     /gene="fumD"
                     /locus_tag="ACHKTW_005183"
     CDS             2051..2260
                     /gene="fumD"
                     /locus_tag="ACHKTW_005183"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HFO6466030.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELEKQAMETVINALVK"
BASE COUNT          616 a          514 c          570 g          644 t
ORIGIN      
        1 ttattgcttc tggttatcga ttaaataaaa aaagcgccca tcagggcgct tcgatataca
       61 aattaattca caaaagcaat attacaggac gtgaacagat gcggtgttag tagtgccgct
      121 cggtaccagt gcaccagaaa ccataactac aacgtcacct ttgtgtgcca gaccgctctg
      181 cagagccagt tctttaccca gacggtagaa atcatcagta gaagtgatct ctttaaccag
      241 ctgcggcaca acgcctttgc tcagtaccaa ctgatgagcc gttttttcgt tggtggtcag
      301 tgccaggatg gtggcatccg ggaagtattt acgtactgcg cgagcagatt taccgccctg
      361 agtagcaacc acgatcagcg gagcatccag tttttcagca gtttcaacgg caccacggca
      421 taccgcttcg gtaatgcgca gtttacggtt gtcattgttg aactcgagac ggctgttcat
      481 cacgcggtcg gtacgttcgc aaatggtcgc catgatagaa accgcttcca gcgggtattt
      541 accttttgcg gattcaccag acagcatcac tgcatcagta ccgtcgagga tggcgtttgc
      601 aacgtcaccg gcttctgcgc gagtcgggcg tgggtttttg atcatggaat ccagcatctg
      661 ggtcgcagtg ataacgactt tacgtgcacg gatacatttt tcgatcatca tcttctgggc
      721 gaagataact tcttctaccg ggatttctac acccaggtcg ccacgcgcaa ccatgatgcc
      781 gtcagaggct tcgaggattt cgtcgaagtt gttgaggcct tcctggtttt cgattttgga
      841 gatgatgtgg atgttttcgc cgccgtgcgc tttcaggtgc tcacggattt cgataacgtc
      901 agaacgctta cgaataaagg aagcagcaac aaagtctacg ccttgttcgc aaccaaagat
      961 caggtcctgt ttgtcttttt cagccagtgc tggcagagca atggaaacgc caggcaggtt
     1021 cacacctttg ttttcgccca ggtcaccgtt gttcagcact ttacagataa ctttgttacc
     1081 ttcaatggcg gtaacttcca taccgatcag accatcgtca accagtacgg tgttgccaac
     1141 tgacaggtca gtagtgaaac cttcatacgt taccgcaacc atttcgctgt tgccgataac
     1201 agatttatca gtggtgaaag taaaggtctg accagctttc agagaaacgt cgttaccgcc
     1261 ttccagtttc atggtgcgga tttccggacc tttggtatca agcaggatag cggcggtttt
     1321 accagttttg ctcatcacgt tgcgcagatt ctgaatgcgc tgaccgtgtt ctgcatagtc
     1381 accatgagag aagttcagac gcataacgtt catgccagcg tccagcattt tagctaacat
     1441 ctcttcagat tcggttttcg gtccgatggt gcaaacaatt ttggtctttt tcatgacagt
     1501 cttagtcttt aagttgagaa ggatgggaga aacttgcttt ctgggcgcac tgccggaaag
     1561 cgaatccggt attacgaaag tggtggtgcc tcgctctaag gataggtgac atcgaaaaag
     1621 cgtgcagagg aatgtgtgct tgtgtctcag cccaacgtga gtgcctgttt ttgagtcgtt
     1681 gagttctaca gtccaaagtg ctgaaaccat tcaagagtca attggcgcgc attatacgct
     1741 gaaattaatc caaaaggaaa acaaaaacag cgtttcaaaa aatatacaac ttgttagaca
     1801 ataaattgtt atcaaagatt taacgttcca gggaaaaggt tacgcaaaaa cttcactgtt
     1861 tgcgcaacgt ctggtgccga agttgtcagt gtgaggataa gtgaaagttc atccggtgta
     1921 taaatacatc atcgctcaat tgctacagtt aaaataattt atcgcgttat ttgtctgatt
     1981 ttgtttgatt tttgtttaat tggttagatt gatatatgct gatagttggc atgataaata
     2041 ggtataaaga atgggaaatc ggacaaaaga ggatgaactg taccgagaaa tgtgcagagt
     2101 tgtcggtaaa gttgtgctgg aaatgcgcga tctggggcag gaaccaaagc atattgttat
     2161 cgcaggcgta ttacgtacag cattagccaa taaacgcatt cagcgcagtg agctggaaaa
     2221 gcaggcgatg gaaaccgtca ttaacgcact ggtaaaatga ccattcagga tgaggcggta
     2281 agcttcatcc tgatattctt gcttcagttg cggtccagtt aatcattcta aataacaact
     2341 gctg
//
LOCUS       DAZFQD010000279         2272 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.284, whole genome shotgun sequence.
ACCESSION   DAZFQD010000279 DAZFQD010000000
VERSION     DAZFQD010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2272
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.284"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..2236
                     /locus_tag="ACHKTW_005184"
                     /pseudo
     CDS             1..2236
                     /locus_tag="ACHKTW_005184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014926.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
BASE COUNT          581 a          570 c          720 g          401 t
ORIGIN      
        1 cacagcgact tcagctacga tgcgctggac cggttggtac agcagggcgg ctttgacggg
       61 cggacgcaac gttatcatta tgacctgacc ggaaaactca cgcagagtga agatgagggg
      121 cttgtcaccc tctggcacta cgatgcgtcg gaccgcatca cgcaccggac ggtgaacggc
      181 gacccggcag agcagtggca gtatgacggc cacggctggc tgagagaaat cagccacctg
      241 agcgaaggcc accgtgtcgc tgtccactac ggctacgacg ataagggccg cctgacgggc
      301 gaacggcaga cggtggagaa cccggagacg ggggaactgc tgtggcagca tgagacgaaa
      361 cacgcctaca acgaacaggg gctggcaaac cgcgtcacgc cggacagcct gccgccggtg
      421 gagtggctga cctacggcag cggttacctg gcgggcatga agctgggcgg gacgccgctg
      481 gtcgagtata cgcgggacag gctgcaccgt gagacggtgc gcagcttcgg cagcatggca
      541 ggcagtaatg ccgcatatga actgaccagc acatacaccc ccgcaggcca gttacagagc
      601 cagcacctga acagcctggt gtatgaccgt gactacgggt ggaatgacaa cggcgacctg
      661 gtgcgcatca gcggtccgcg acagacgcgg gaatacggct acagcgccac gggcaggctg
      721 gagagcgtgc gcaccctcgc accggacctg gacatccgca tcccgtatgc caccgacccg
      781 gcgggaaacc gcctgccgga cccggagcta cacccggaca gcacgctcac ggcgtggccg
      841 gataaccgca tcgcagagga tgcgcactat gtttaccact atgatgaata cggcagactg
      901 acggagaaga cggaccgtat cccgacgggt gtgatacgga tggacgacga gcggacccac
      961 cactaccact acgacagcca gcaccgcctg gtgttccaca cgcggataca gcatggcgag
     1021 ccactggtcg agagccgcta cctctacgac ccgctgggac ggcgaatggc gaaacgggta
     1081 tggcggcggg agcgtgacct gacggggtgg atgtcgctgt cgcgtaaacc ggaggagaca
     1141 tggtacggct gggacggcga caggctgacg acggtacaga ccgacaccac gcgtatccag
     1201 acggtatacc agccggggag cttcgcgccg ctcatccgca tcgaaacaga caacggtgaa
     1261 cgggagaaag cgcagtgccg cagcctggcg gagaagttac agcaggaagg gagcgaagac
     1321 ggtcacggtg tggtgttccc ggctgaactg gtggggctgc tggacaggct ggagggggaa
     1381 atccgggcaa actgcgtgag cagtgaaagc cgccagtggc ttgcgcagtg cgggctgacg
     1441 gtggagcggc tggcggcaca gatagagccg gtttatttgc ctgagcgaaa aatacatctt
     1501 tatcactgtg accaccgggg cctgccgctg gcgcttatca gtgaagacgg caatacggcg
     1561 tggagcgcgg aatatgatga atggggcaac cagcttaatg aggagaaccc gcatcacctg
     1621 caccagccgt accggctgcc ggggcagcag tatgataagg agtcggggct gtactacaac
     1681 cggaaccggt actacgatcc gttgcagggg cggtatatca ctcaggaccc gatagggctg
     1741 gaggggggga tggagtctgt atgcgtaccc gctgaatccg gtgaatggta ttgatccatt
     1801 agggttaagt cccgcagatg tagcgctaat aagaagaaaa gatcaactaa accatcaaag
     1861 agcatgggat atattatctg atacttatga agatatgaag agattaaatt taggtgggac
     1921 tgatcaattt ttccattgta tggcattttg tcgagtgtct aaattaaatg acgctggtgt
     1981 tagccgatcg gcgaaagggc tgggttatga aaaagagatt agagattacg ggttaaatct
     2041 gttcggtatg tacggcagaa aagtaaagct atcccattct gaaatgattg aagataataa
     2101 aaaagacttg gctgtaaatg accatgggtt gacatgtcca tcaacaacag attgctcaga
     2161 tagatgtagt gattatatta atccagagca taaaaaaacg ataaaggctt tacaagatgc
     2221 tggctatctc aagtaatcta tcaaagatga taatatttat ttttgctatt at
//
LOCUS       DAZFQD010000280         2269 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.285, whole genome shotgun sequence.
ACCESSION   DAZFQD010000280 DAZFQD010000000
VERSION     DAZFQD010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2269
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.285"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1620
                     /locus_tag="ACHKTW_005185"
     CDS             <1..1620
                     /locus_tag="ACHKTW_005185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6466031.1"
                     /translation="STRKIDLAGEKALLGVDVPDSLDLPGDMPVFLDYQARWFEDESE
                     VCIAEKSRRTGLTWAEAGRNVITAAKPKRRGGRNVFYVGSKQEMALEYISACALFSRA
                     FNQLADADVYEQTFWDRDKKEEILTYMIRFPNSGFKIQALSSRPSNLRGLQGDVVIDE
                     AAFHESLDELLKAAMALTMWGARVRIISTHNGVDNLFNQYIQEAREGRKDYSVHRITL
                     DDAIADGLYRRICYVTGREWSPESEQKWRDDLYKNAPTREDADEEYGCIPKKSGGAYI
                     PHALIEMAMIRDIPILTFEAPDDFISRAAWLRESEVLTWCEEHLKPLLEALNPRSRFS
                     FGEDFARTGDLSCFVLLEITESLAKREVFRVELRNLPYAQQEQVMMYILTRVPALVGA
                     AFDATGNGGYLAEAALLAFGPDIIDCVMLSPKWYGEWMPKLKAEFEDQNILVARHQTT
                     LDDLRHVKVVNGIPQIDKGRTKDQNATAANARRHGDFAVALCMANRASYMEGFILDES
                     ACQALPERSRAMEGGYRDDDEAYHEFDRGCW"
     gene            1620..>2269
                     /locus_tag="ACHKTW_005186"
     CDS             1620..>2269
                     /locus_tag="ACHKTW_005186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312997.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF935 family protein"
                     /protein_id="HFO6466032.1"
                     /translation="MGRIIDLDGKPFSFDPEMQSTALDIPQIASRYVEHPASGITPNR
                     AAQCLRGAERGDLIAQSDLAADIEEKDTHLFAELGKRRLAIQGVPWSIEPPPNASANE
                     KKDAEMLDEYLHSADWFDAMLFDATDAILKGYSCMEIEHGMLGKMHIIRAIRWRDSGH
                     FCLNPDDLSELRLRDGSHAGVAFQPFGWIVHQSRSRTGYGGATGLVRTLIWPFIFK"
BASE COUNT          490 a          599 c          678 g          502 t
ORIGIN      
        1 tccacccgca aaattgatct ggccggtgaa aaagccctgc tgggcgtgga tgtgccggac
       61 agccttgatt tgcccggtga tatgccggtg tttctggatt atcaggcccg ctggtttgag
      121 gatgaaagcg aggtctgcat cgcggaaaaa tcccgccgta ccgggcttac ctgggcggaa
      181 gccgggcgta acgtcattac cgctgcaaaa ccgaagcggc gcggtggccg caatgtgttt
      241 tatgtggggt caaagcagga gatggcgctg gaatatattt ctgcctgcgc cctgttctcc
      301 cgtgccttta accagctggc agatgctgat gtgtatgagc agaccttctg ggatcgggat
      361 aaaaaagaag aaattctgac ctacatgatc cgctttccga acagtggatt caaaattcag
      421 gcactgtctt cccgtccgtc aaacctgcgc ggccttcagg gagatgtggt gattgatgaa
      481 gccgcgttcc acgagtcact ggatgagctt ctcaaggcgg caatggcgct taccatgtgg
      541 ggcgcgcgcg tgcgtattat ctccacgcat aacggcgtcg ataatctgtt taatcagtac
      601 attcaggaag cccgcgaggg acgcaaggat tacagcgtcc accgcataac cctggatgat
      661 gccattgcgg acgggctgta ccgtcgtatc tgttatgtca ccggccgcga atggtcaccg
      721 gaaagcgagc agaagtggcg tgatgatctc tacaaaaacg ccccgacccg tgaggatgcc
      781 gacgaggaat acggctgtat cccgaaaaaa tccggcggtg cctatattcc tcacgcactt
      841 attgaaatgg cgatgatccg cgacatcccg attctgacgt ttgaagcccc ggacgacttc
      901 atcagccgtg ccgcatggct gcgtgaatcg gaagttttaa cctggtgtga agaacattta
      961 aaaccgcttt tagaggcgtt aaatccccgt tcccgcttca gcttcgggga agactttgcc
     1021 cgcaccggtg acctctcctg ctttgtgctg ctggaaatca ccgaatccct ggcaaaacgt
     1081 gaggtgtttc gcgtggagct gcgcaacttg ccgtatgccc agcaggagca ggtgatgatg
     1141 tacatcctga cccgcgttcc ggccctggtt ggtgcagcgt ttgacgccac cggtaacggc
     1201 ggctatctgg cagaagccgc gctgctggcc tttggtccgg acatcatcga ctgcgtgatg
     1261 ttgtcgccga agtggtacgg cgagtggatg ccaaaactga aagccgagtt tgaagatcag
     1321 aatatcctcg tcgcccgcca tcagaccacg cttgatgatt tgcgccatgt gaaggtggtg
     1381 aatggtatcc cgcaaatcga caaggggcgc acgaaggacc agaacgcaac ggctgcgaac
     1441 gcccgccgcc atggtgattt tgccgtggca ctctgtatgg caaaccgggc gtcatacatg
     1501 gagggtttca tcctggatga atcagcctgt caggcgctgc cggagcggtc gcgggcaatg
     1561 gagggcggtt atcgtgatga cgatgaggca tatcatgaat ttgatcgggg gtgctggtag
     1621 tgggacgcat aattgatctt gacggaaaac ctttctcctt tgacccggag atgcaaagta
     1681 ccgcgctgga tattccgcag attgccagcc gttatgttga gcatcccgcc tcgggtatca
     1741 ccccgaaccg ggcggcgcag tgccttcgcg gggctgaacg tggcgatctg attgcccagt
     1801 ccgatctggc ggctgacatt gaagaaaagg acacccacct ttttgcggag ctgggcaagc
     1861 gacgtcttgc cattcagggg gtgccatgga gcattgagcc gccaccgaac gccagcgcga
     1921 atgagaaaaa ggacgcggaa atgctcgacg aatatctgca ttccgcagac tggtttgatg
     1981 ccatgctgtt tgacgccacg gatgccatcc tgaagggcta ttcctgcatg gaaattgagc
     2041 acgggatgct cggtaaaatg cacatcatcc gcgccatccg ctggcgtgac agcgggcatt
     2101 tctgccttaa cccggatgat ttgagcgaac tgcgactgcg tgacggcagc catgccgggg
     2161 tggcgtttca gccctttggc tggatagtgc atcagtcgcg ttcacgcacc ggctacggtg
     2221 gtgcgacggg gcttgtcaga acgcttatct ggccgttcat tttcaaaaa
//
LOCUS       DAZFQD010000281         2244 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.286, whole genome shotgun sequence.
ACCESSION   DAZFQD010000281 DAZFQD010000000
VERSION     DAZFQD010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2244
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.286"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(5..328)
                     /locus_tag="ACHKTW_005187"
     CDS             complement(5..328)
                     /locus_tag="ACHKTW_005187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4761 family protein"
                     /protein_id="HFO6466033.1"
                     /translation="MKQQRNARINNNTERHVARQHFRKNSATATTEIHTTPDGHQIKQ
                     TGKHTWAIGDTGIVIHKSFRSPVTGRINFTLTRGDDYFGQDFTFFEALRTVDRLMTGR
                     NFHAK"
     gene            complement(354..548)
                     /locus_tag="ACHKTW_005188"
     CDS             complement(354..548)
                     /locus_tag="ACHKTW_005188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6466034.1"
                     /translation="MTDSTQTKDLYSDLRFIIIQQQSGRLDPTRASKAWKEALTDQGI
                     DVISEVMAEVIFKLTRDESL"
     gene            complement(616..819)
                     /locus_tag="ACHKTW_005189"
     CDS             complement(616..819)
                     /locus_tag="ACHKTW_005189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO6466035.1"
                     /translation="MQGITININVNAPYVSLKKYAEITGIPLNTCKKMLADGRIIIRP
                     KRAKMEKPEVNLIAMLKDAIANS"
     gene            891..1028
                     /locus_tag="ACHKTW_005190"
     CDS             891..1028
                     /locus_tag="ACHKTW_005190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6466036.1"
                     /translation="MRAMLEDERCSLVAISSMTARSSADNRSASRGPVAVLRGIRDLV
                     S"
     gene            1118..1522
                     /locus_tag="ACHKTW_005191"
     CDS             1118..1522
                     /locus_tag="ACHKTW_005191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO6466037.1"
                     /translation="MKNDIGQRLREERERLGLSQVAMSDIGGVKKLTQLRYEKGDSFP
                     DAAYLSALSRFGLDVQYVVLGIHSPETYNDDEQELITRFRSASLDVKNAVIGALKGAV
                     TEKESQSSGGGLNISGGNNRIAGRDYNETKGK"
     gene            1538..2182
                     /locus_tag="ACHKTW_005192"
     CDS             1538..2182
                     /locus_tag="ACHKTW_005192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6466038.1"
                     /translation="MTVNSNGSDNRIAGRDYQEKNIQIGRYDGSHTVNIAIPSSNNSD
                     PRPLLKGQRKALNRLVAAIVDASNTEAFIIWQKLHAEIGVDSIDDITMNQYKTAESFL
                     CAMLERCKDHDARKALVSILLRNSEDGVLRQKLIRYCHINFGTGRLNDLTRSQLQMAL
                     SWLDQQSVEGEKKLTANNSAGSQLQMNFQQIIRKYPKETGMCFVVGVLLGSVLF"
BASE COUNT          587 a          410 c          546 g          701 t
ORIGIN      
        1 tgtatcattt agcgtgaaaa ttacgaccag tcattaaccg atcgaccgtt cttaaggctt
       61 caaaaaatgt gaagtcctgt ccgaagtaat catctccgcg agtcagggta aaattaattc
      121 tgccagtaac agggctacga aaacttttgt gaataacgat gccagtgtcg ccaatcgccc
      181 aggtgtgctt tccggtttgt tttatctgat gaccgtctgg tgtggtatgg atttcagtgg
      241 ttgcggtcgc actattttta cggaagtgct ggcgtgcaac gtggcgttcg gtgttgttat
      301 tgatgcgtgc atttcgttgt tgcttcattc ttcactcggt ccttatgcga tgattacagt
      361 gattcgtctc ttgttagctt aaaaatcact tcagccatga cttcgctaat tacatcgatc
      421 ccctgatcgg taagtgcttc tttccaggcc ttgcttgcac gtgttggatc cagtctgcct
      481 gattgctgtt gtatgatgat gaatcgtaaa tcgctgtata agtcttttgt ttgagtggag
      541 tcagtcatgt caacaccttc aaatcttgat gtttttaatg aaacgttttc ttcatagtta
      601 tgctcgcttc attgtttagc tgttggctat tgcgtctttt aacatagcta taaggtttac
      661 ttctggtttt tccattttgg cgcgtttggg gcggataata attcgaccgt cagccaacat
      721 ctttttgcat gtgttaagag gaatgcctgt tatctcggcg tatttcttca gagatacgta
      781 aggggcatta acgttgatgt ttattgttat accttgcatc ccgttaacct cctgagcatg
      841 aagattggtt ttgctctttc tggataatct caaggccacg aaggaagatc atgcgtgcca
      901 tgttagagga tgagcgctgt tctttagttg ccatttcatc aatgacggct cgctcttctg
      961 ctgacaatcg aagtgccagt cgcggtcctg tagctgtgtt acgcggaatc cgtgatctgg
     1021 tgtcgtgaac aacttgtttc atagtggtat attgtgttca tctaatggct catgaaagta
     1081 ttttggtatc aaaaaagata cctgtcaagg tttttgtatg aaaaatgata ttggccaacg
     1141 gttgcgtgag gaaagggaac ggctgggtct tagccaagtc gctatgagtg atattggtgg
     1201 ggttaaaaag ttaacccaac ttagatatga gaaaggggat agctttcctg atgcggcgta
     1261 cttatcagcg ttgtcgcgtt ttggccttga tgttcagtat gtcgtgttgg gaattcattc
     1321 acctgaaact tataacgatg atgagcagga gttgatcaca cgttttcgga gtgcctcact
     1381 agatgtgaaa aacgcggtga ttggtgcttt gaaaggtgca gtcactgaaa aggagagtca
     1441 gtcatcaggg ggagggttaa atatctctgg cgggaataac cgcatcgcag gccgtgacta
     1501 taacgaaaca aagggtaagt gatagcaagg aggtggcatg acggtcaact caaacggttc
     1561 agataatcgt attgctgggc gagattacca agaaaagaat attcagatag ggcgatatga
     1621 tggctctcat accgtcaata tcgcaattcc ttcgagcaat aacagtgatc cacgcccctt
     1681 acttaagggg caacggaagg cattaaatag attagttgct gctattgtag atgccagcaa
     1741 taccgaggcg tttattattt ggcaaaaatt gcatgcagag attggtgtag acagtattga
     1801 tgatataaca atgaatcaat ataagaccgc agagagcttt ctgtgtgcga tgcttgaacg
     1861 atgtaaagat catgatgcgc gtaaggcgct tgtaagtatt ttacttcgta atagtgaaga
     1921 tggagtgctt cgtcaaaaac ttatccgcta ttgccatatc aatttcggaa cgggtcgctt
     1981 aaacgatctt actcgttctc agcttcagat ggctctttcc tggttagacc aacaatcagt
     2041 ggaaggagaa aagaaattaa ctgccaacaa ttctgccggt agtcaattac agatgaattt
     2101 tcagcaaata attagaaaat acccgaaaga aacaggaatg tgtttcgttg ttggtgtttt
     2161 attaggcagt gttcttttct gatagatagt tgttggagaa agatattatg acaaaagctt
     2221 gctttaccga ctttaaaaga aatg
//
LOCUS       DAZFQD010000282         2237 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.287, whole genome shotgun sequence.
ACCESSION   DAZFQD010000282 DAZFQD010000000
VERSION     DAZFQD010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2237
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.287"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..996
                     /locus_tag="ACHKTW_005193"
     CDS             <1..996
                     /locus_tag="ACHKTW_005193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313014.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HFO6466039.1"
                     /translation="VNETTLNAIAYALAGLSAQEHEHLAWISRQIIPTEADEAELLKH
                     CAFWGVIRKPASRADGPVQLMLTTDAGITEGVLLQRSDGVVYRITGSATGKAGTLNVN
                     VEAESAGRAGNAPPGTKLTFITPQAGINQTTTVTGTGLTGGADVETVPELLSRLVFRV
                     QNPPSGGTQYDFERWAREVPGVTRAWCRPEWPQAGSVGVTFVQDNNPDIFPGEGDVKR
                     VADYIRSHDDPATGQPVGQPLGPTISVFKLTNKPVVFEIRIVPKTPENQAAVKQALTD
                     LLYNESRPGGLVLPSSFWRAVAGVKGLEDFDVRSPLKSVMAGDTELLTVGEITWL"
     gene            987..1544
                     /locus_tag="ACHKTW_005194"
     CDS             987..1544
                     /locus_tag="ACHKTW_005194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative phage tail protein"
                     /protein_id="HFO6466040.1"
                     /translation="MAVTLTPHQRALLQLLPDGLAWDKRPSSVLAALCLGLSHSTERV
                     SWIGNQMLAERFPDSSRLLLEDWERYLGLPECDMTGATIQERQRYAGNKYRMKPSLNR
                     EFYIRFATEFGYEIDIQPSPESQWISIVTVKTAVGYRHMNVLDDILTPLRIYDASALE
                     CILNRYKPAWQTFLYLYENSHEETE"
     gene            1544..>2237
                     /locus_tag="ACHKTW_005195"
     CDS             1544..>2237
                     /locus_tag="ACHKTW_005195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO6466041.1"
                     /translation="MYFVDNNSGVTDMPPLAPSQGTQVKWFTEGDGRKGISHIGQDWL
                     NIVQAELLAILTEGKVQPDKAKLNQLVTAIKAIIAANAYSRKNNLKEIADAGAEAQAA
                     ARRHLGLGGLSGKDSLAAADVGALEKSRNFDDVLDKPTARLNLDVYSKGEGDARYLRR
                     DQNGADIPDKGTFINNLGLRETVNKAADALPSGGTAVAANRLATPVNINGVPFDGTQD
                     INITSGMTQSTAD"
BASE COUNT          550 a          546 c          668 g          473 t
ORIGIN      
        1 gtgaatgaaa ccaccctgaa tgccattgcg tatgcactgg cgggtctgtc agcacaggag
       61 catgaacatc tggcctggat ctcccggcag ataattccga cagaagctga tgaagccgaa
      121 ctcctgaaac actgcgcatt ctggggtgtc atccgtaaac cggcttcccg agcagacgga
      181 ccggtacaac tgatgctgac cacggatgca gggatcacag aaggtgtact ccttcagcga
      241 agcgatggtg ttgtgtaccg catcaccggc tctgcgaccg gaaaagccgg aacactgaat
      301 gttaatgtgg aggcggaaag tgcggggcgc gccggaaatg ccccgccagg gacaaaactg
      361 acatttatca caccgcaggc ggggatcaac cagacgacca cggtgaccgg cacgggactc
      421 accggtggtg cggatgtgga aaccgtgccg gagctgcttt cccgcctggt tttccgggta
      481 cagaatccgc cgtccggtgg cacgcagtac gattttgaac gctgggcgcg tgaggtgccg
      541 ggcgtgacgc gggcatggtg ccgcccggaa tggccgcagg cgggcagcgt gggtgtaacg
      601 ttcgttcagg acaacaaccc tgacattttc cccggtgaag gtgatgtgaa gcgggtggcg
      661 gattatatcc gcagtcatga tgatccggcg acgggccagc ccgttggtca gccacttggg
      721 ccgacaatca gcgtgtttaa gctgaccaat aagccggtgg tgtttgagat aaggattgta
      781 cccaaaacgc cggaaaatca ggctgccgta aaacaggcat taacggacct gctttataac
      841 gaatcgcggc cgggtggact ggtgctgcct tcatcattct ggcgggctgt tgcaggggtg
      901 aaaggactgg aagattttga cgttcgaagt ccgctgaaat ccgtgatggc cggagataca
      961 gagttgctga ccgtggggga aatcacatgg ctgtaaccct gaccccgcat cagcgcgccc
     1021 tgctgcagtt gctgcctgac ggtctggcat gggacaagcg gccgtcatcc gttcttgcgg
     1081 ctttgtgcct gggtctcagt cattccacgg agcgtgtttc ctggatcggt aaccagatgc
     1141 tggcagaacg ttttcctgat tcatcccgtc tgctgctgga agactgggag cgttatctgg
     1201 ggttaccgga atgtgatatg accggcgcaa ccattcagga gcgtcagcgt tatgccggga
     1261 ataaataccg gatgaaaccc tctcttaacc gtgaatttta tatccggttt gcaacagagt
     1321 ttggttatga aatagatatc cagccatcac cggaatcaca atggattagt attgtgacag
     1381 tgaagacggc ggtgggatac cgtcacatga atgtactgga cgatattctt acgccactgc
     1441 gaatttatga tgcaagtgcg ctggagtgca ttttaaaccg ttataagcca gcctggcaga
     1501 cgtttttata tctgtacgaa aacagtcatg aggagacgga gtaatgtatt ttgtcgacaa
     1561 caattcaggt gtgaccgata tgccaccact ggccccctct cagggcacgc aggttaaatg
     1621 gtttactgaa ggtgacggac gcaaagggat cagccatatc ggtcaggact ggctgaacat
     1681 cgtgcaggca gagctgcttg ccattctgac agaaggaaag gttcagccgg ataaggcaaa
     1741 actgaaccag ctggtgacag ccattaaggc aatcattgca gcaaacgcat acagccggaa
     1801 aaacaacctt aaggagattg cggatgcagg tgcagaagca caggctgctg cccgccgtca
     1861 tctggggctg ggaggattat caggaaaaga cagtctggct gctgctgatg tgggagcact
     1921 ggagaaatcc cggaattttg acgatgtact ggataaaccg acggctcgtc tgaatcttga
     1981 tgtttacagt aagggagaag gcgatgcgcg ttatctgcgc agggaccaga atggcgcgga
     2041 tatcccggac aaaggaacct ttatcaataa cctcggttta cgggaaacgg taaataaggc
     2101 tgctgatgcc ctgccatcgg gtggaaccgc cgtggcagca aacagactcg ccaccccagt
     2161 aaatattaat ggtgttcctt tcgacgggac gcaggatatc aacatcacgt caggaatgac
     2221 gcagtcaacc gcagatg
//
LOCUS       DAZFQD010000283         2236 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.288, whole genome shotgun sequence.
ACCESSION   DAZFQD010000283 DAZFQD010000000
VERSION     DAZFQD010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2236
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.288"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>2236)
                     /locus_tag="ACHKTW_005196"
     CDS             complement(<1..>2236)
                     /locus_tag="ACHKTW_005196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFO6466042.1"
                     /translation="HSDFSYDALDRLVQQGGFDGRTQRYHYDLTGKLTQSEDEGLVTL
                     WYYDESDRITHRTVNGEPAEQWQYDDHGWLTDISHLSEGHRVAVHYGYDDKGRLTGER
                     QTVENPETGELLWQHETTHAYNEQGLANRVTPDSLPPVEWLTYGSGYLAGMKLGDTPL
                     LEYTRDRMHRETVRSFGSMAGSNAAYKLTSTYTPAGQLQSQHLNSLVYDRDYGWNDNG
                     DLVRISGPRQTREYGYSATGRLESVRTLAPDLDIRIPYATDPAGNRLPDPELHPDSTL
                     TAWPDNRIAEDAHYVYHYDEYGRLTEKTDLIPAGVIRTDDERTHHYHYDSQHRLVFYT
                     RIQHGEPLVESRYLYDPLGRRMAKRVWRRERDLTGWMSLSRKPEMTWYGWDGDRLTTV
                     QTDTTRIQTVYQPGSFAPLIRIETDNGEREKAQRRSLAEKLQQEGSEDGHGVVFPAEL
                     VRLLDRLEEEIRADRVSSESRAWLAQCGLTVEQLARQVEPEYTPARKVHFYHCDHRGL
                     PLALISEDGNTAWRGEYDEWGNQLNEENPYYLHQPYRLPGQQHDEESGLYYNRNRYYD
                     PLQGRYITQDPIGLAGGWNLYNYPLNPIIRMDPLGLYNLYQLLYDVWHDDSYGTSSID
                     ITGSGDLISLGGHAGLGVAFAKKKGEMLSDICIYATACGHAGIGGGINAAITYSETKS
                     LPTSGVSNSVGVTVGGGVGGHFAYTYVVDVDNPESSTESVGIGAGVDASVMTLACRTW
                     QECWV"
BASE COUNT          404 a          717 c          579 g          536 t
ORIGIN      
        1 tgacccagca ttcttgccac gttctacaag ccagagtcat aactgaagcg tcaacacctg
       61 caccgatacc aacagattct gtcgatgatt ctggattatc aacatccact acataagtat
      121 acgcaaaatg ccccccaact ccgccgccaa ccgttacacc tactgaattg ctgactcccg
      181 atgtaggtaa agacttggtc tcagaatatg tgatagccgc atttatccca ccaccaattc
      241 ctgcatgtcc gcatgctgta gcataaatac aaatatcaga taacatttca cctttctttt
      301 tagcaaacgc aacgccaagt cctgcatgac cacctaatga tattagatct ccactgccag
      361 taatatcaat tgatgatgtt ccatatgaat catcatgcca aacatcatat aataattgat
      421 ataaattata caaacccaaa ggatccatcc ttattatcgg attcagtggg taattataca
      481 ggttccatcc ccccgccagc ccaatggggt cttgtgtaat atacctcccc tgtagcgggt
      541 catagtaccg gttccggtta tagtacagcc ctgattcctc atcatgctgc tgccccggca
      601 gacggtatgg ctggtgcagg taatacgggt tctcttcatt aagctggttg ccccattcat
      661 catactcccc gcgccacgcc gtattgccgt cttcgctgat gagcgccagc ggcaggcccc
      721 ggtggtcgca gtggtaaaaa tgaacttttc gcgccggtgt gtattccggc tccacctgtc
      781 tggccagttg ctccaccgtc agcccgcact gcgcaagcca cgcccggctt tcactgctca
      841 cgcggtctgc ccggatttct tcctccagcc tgtccagcag ccgcaccagt tcagccggaa
      901 ataccacgcc gtgcccgtcc tcgctccctt cctgctgcag cttctccgcc aggctgcggc
      961 gctgcgcttt ctcccgctcg ccgttgtctg tttcgatgcg gatgagcggc gcgaagctcc
     1021 ccggctgata taccgtctgg atacgcgtgg tgtcggtctg taccgtcgtc agccggtcgc
     1081 cgtcccaccc gtaccacgtc atctccggtt tacgcgacag cgacatccac cccgtcaggt
     1141 cacgctcccg ccgccatacc cgtttcgcca ttcgccgtcc cagcgggtcg tagaggtagc
     1201 ggctctcgac cagtggctcg ccatgctgta tccgcgtgta gaacaccaga cggtgctggc
     1261 tgtcgtagtg gtagtggtgg gtccgctcgt cgtccgtccg gataacaccc gccgggataa
     1321 ggtccgtctt ctccgtcagt ctgccgtatt catcatagtg gtaaacatag tgcgcatcct
     1381 ctgcgatgcg gttatccggc cacgccgtga gggtactgtc cgggtgcagc tccgggtccg
     1441 gcaggcggtt tcccgccggg tcggtggcat acgggatgcg gatgtccagg tccggtgcga
     1501 gggtacgcac gctctccagc ctgcccgtgg cgctgtagcc gtattcccgc gtctgtcgcg
     1561 ggccgctgat gcgcaccagg tcgccgttgt cattccaccc gtagtcacgg tcatacacca
     1621 ggctgttcag gtgctggctc tgtaactggc ctgcgggggt gtatgtgctg gtcagtttat
     1681 atgcggcatt actgcctgcc atgctgccaa agctgcgcac cgtctcccgg tgcatgcggt
     1741 cgcgcgtgta ctccagcagc ggcgtgtcgc caagcttcat gcccgccaga taaccgctgc
     1801 cgtaggtcag ccactccacc ggcggcaggc tgtccggcgt gacgcggttt gccagcccct
     1861 gttcgttgta ggcgtgtgtt gtctcatgct gccacagcag ttcccccgtc tccgggttct
     1921 ccaccgtctg ccgttcgccg gtcaggcggc ccttatcgtc gtagccgtag tggacagcga
     1981 cacggtggcc ttcgctcagg tggctgatgt ctgtcagcca gccgtggtca tcatactgcc
     2041 actgctctgc cggttcgccg ttcaccgtgc ggtgagtgat acggtccgat tcatcgtagt
     2101 accagagggt gacaagcccc tcatcttcac tctgcgtgag ttttccggtc aggtcataat
     2161 gataacgttg cgtccgcccg tcaaagccgc cctgctgtac caaccggtcc agcgcatcgt
     2221 agctgaagtc gctgtg
//
LOCUS       DAZFQD010000284         2198 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.289, whole genome shotgun sequence.
ACCESSION   DAZFQD010000284 DAZFQD010000000
VERSION     DAZFQD010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2198
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.289"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(49..390)
                     /locus_tag="ACHKTW_005197"
     CDS             complement(49..390)
                     /locus_tag="ACHKTW_005197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000247831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO6466043.1"
                     /translation="MVNMSRDYAKKLKAIRNAENLTQSQFSQVTGVSVGTIGNYESGY
                     KPARIEVVEKVIQVERFEKYTMWILHDKTFPAAGQVAPALSLDGSIQSEGDQVSIGIT
                     QKSPRSRRNAG"
     gene            494..1018
                     /locus_tag="ACHKTW_005198"
     CDS             494..1018
                     /locus_tag="ACHKTW_005198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO6466044.1"
                     /translation="MRKSIADKELSDTQALQENDSITESVGAANDDEHQEEKKKRTYK
                     KGATLRLDGPVAGLCSLEKGAAYIGLTKAALRVALQRNQMPGHKTRSNPEDENSDGVW
                     WFNAREWDKLADELPECEPPEWHNWKSYWTYDRQKRKFSPANKEDCQTINGKRVYTGR
                     KSKLEQHQYSEGNQ"
     gene            1070..1264
                     /locus_tag="ACHKTW_005199"
     CDS             1070..1264
                     /locus_tag="ACHKTW_005199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6466045.1"
                     /translation="MTDSTQTKDLYSDLRFIIIQQQSGRMDPTRASKAWKEALTDQGI
                     DVISEVMAEVIFRLTRDESL"
     gene            1290..1613
                     /locus_tag="ACHKTW_005200"
     CDS             1290..1613
                     /locus_tag="ACHKTW_005200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4761 family protein"
                     /protein_id="HFO6466046.1"
                     /translation="MKQQRNARINNNTERHVARQHFRKNSATATTEIHTTPDGHPVKQ
                     TGKHTWAIGDTGIVIHKSFRSPVTGRFNFTLTRGDDYFGQDFTFFEALRTVDRLMTGR
                     NFHAK"
     gene            1627..1842
                     /locus_tag="ACHKTW_005201"
     CDS             1627..1842
                     /locus_tag="ACHKTW_005201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6466047.1"
                     /translation="METYKIKNIEHHDGFFSTEHVACVVRIFNEVDVILKQNIKLEII
                     AKSEADAIEIKKAIERQIMPLTSIIAF"
     gene            1861..2100
                     /locus_tag="ACHKTW_005202"
     CDS             1861..2100
                     /locus_tag="ACHKTW_005202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4754 family protein"
                     /protein_id="HFO6466048.1"
                     /translation="MTRNYKEIVNQIIKAYSHRIAKSPEFIRIMETRSLMQGLNGLAL
                     AALAANDIDATIEINNLLLDIEKGGNIEPYNLEAA"
     gene            2097..>2198
                     /locus_tag="ACHKTW_005203"
     CDS             2097..>2198
                     /locus_tag="ACHKTW_005203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000021655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6466049.1"
                     /translation="MSVFLSWVILIISVLMTIGIMRIIHTLKDIELFF"
BASE COUNT          719 a          498 c          492 g          489 t
ORIGIN      
        1 aatcagccgt acactctcta cgggatgaaa cccgctcgat gtacagcgtt agccagcatt
       61 gcgacgagat cggggtgatt tttgggtaat gccgattgaa acctgatcgc cctccgactg
      121 aatggagcca tcaagagaga gagccggggc aacctgcccg gcagcgggaa atgttttgtc
      181 gtgaagtatc cacatagtgt atttttcgaa tcgctcgact tggattactt tttctacaac
      241 ctcaattcgc gcaggtttat atccagactc ataatttcct atagttccta cagaaactcc
      301 cgttacttgc gaaaactgag attgtgtaag gttttccgcg tttctgattg cctttagttt
      361 tttggcgtaa tctcttgaca tgttcaccat acgatgagta tcctctattc gaacttcacc
      421 acagggtgaa gtaaacaaat caaaacaaac cttcatgatg cgcgacaagc gcaccaacgc
      481 aagaggatag caaatgcgga agtcaattgc cgataaagag ttatctgaca cacaagccct
      541 tcaggaaaac gacagcatca ccgaatccgt tggcgcggca aacgacgatg aacaccaaga
      601 ggaaaagaaa aaacgcacct acaaaaaagg ggcaaccttg cgactggatg gcccggtagc
      661 cggactttgc tcacttgaaa aaggggcagc ctatatcggg ttgacaaaag ctgcattgcg
      721 cgtggcgctt cagcgaaatc agatgcctgg acacaagacc cgttcaaacc ctgaagatga
      781 aaattcggat ggcgtatggt ggtttaacgc cagagagtgg gacaagctgg ctgacgaact
      841 accagaatgc gagccgccag aatggcacaa ctggaaaagc tactggacgt acgaccgaca
      901 gaaaagaaag ttttccccgg caaacaaaga agactgccag accatcaatg gaaagcgggt
      961 ttacacagga agaaagtcaa aactggagca acaccaatac agcgaaggca accagtgaca
     1021 tcaaaacaaa ccgggatcat taagagcatc gggatttgaa tgtgttgaca tgactgactc
     1081 cactcaaaca aaagacttat acagcgattt acgcttcatc atcatacaac agcaatcagg
     1141 caggatggat ccaacacgtg caagcaaggc ctggaaagaa gcccttaccg atcaggggat
     1201 cgatgtaatt agcgaagtca tggctgaagt gatttttagg ctgacaagag acgaatcact
     1261 gtaatcatcg cataaggacc gagtgaagaa tgaagcaaca acgaaatgca cgcatcaata
     1321 acaacaccga acgccacgtt gcacgccagc acttccgtaa aaatagtgcg accgcaacca
     1381 ctgaaatcca taccacacca gacggtcatc cggtaaaaca aaccggaaag cacacctggg
     1441 cgattggcga cactggcatc gttattcaca aaagttttcg tagccctgtt actggcagat
     1501 ttaattttac cctgactcgc ggagatgatt acttcggaca ggacttcaca ttttttgaag
     1561 cgttaagaac ggtcgatcgg ttaatgactg gtcgtaattt tcacgctaaa tgatacagat
     1621 gaaaacatgg aaacatacaa aattaaaaat attgaacacc atgacggttt tttcagcact
     1681 gaacatgttg cttgtgttgt caggattttc aatgaagtag atgtgatttt gaagcaaaac
     1741 ataaagcttg aaatcatcgc aaaaagcgaa gccgacgcca ttgaaataaa aaaagctatt
     1801 gaacgccaga taatgccgtt gacaagtatc attgcatttt aagcaacagg aaaaataacc
     1861 atgaccagaa attacaaaga aatcgtcaat cagatcatta aggcttacag ccaccgcatc
     1921 gcaaaatcac ccgagttcat acgcataatg gagacgcgct cattaatgca aggactgaac
     1981 gggttggcac tggccgcatt ggcagctaac gatattgatg ccactatcga aataaacaac
     2041 ctgttattag acattgaaaa aggcggaaac atagaaccct acaatctgga ggccgcatga
     2101 gcgtatttct ttcgtgggta attctgatta tctccgtgct tatgacaatc ggcattatgc
     2161 gaattatcca cacactgaag gatatcgaat tattcttt
//
LOCUS       DAZFQD010000285         2153 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.290, whole genome shotgun sequence.
ACCESSION   DAZFQD010000285 DAZFQD010000000
VERSION     DAZFQD010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2153
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.290"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            174..>2153
                     /gene="yjcS"
                     /locus_tag="ACHKTW_005204"
     CDS             174..>2153
                     /gene="yjcS"
                     /locus_tag="ACHKTW_005204"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase YjcS"
                     /protein_id="HFO6466050.1"
                     /translation="MNNSRLFRLSRIVIALTAASGMMVNTAYATDEAKAATQYTQQVN
                     QNYAKSLPFSDRQDFDDAQRGFIAPLLDEGILRDANGKPYYRGEDYKFDINAPAPETV
                     NPSLWRQSQLNGISGLFKVTDRMYQVRGQDISNITFIEGDTGIIVIDPLVTPPSAKAA
                     LDLYFQNRPQKPIVAVIYTHSHTDHYGGVKGIISEADVKSGKVQVIAPAGFMDEAISE
                     NVLAGNIMSRRALYSYGLLLAHNPQGNIGNGLGVTLASGYPSIIAPNKTITKTGEKMI
                     IDGLEFDFLMTPGSEAPAEMHFYIPALKALCTAENATHTLHNFYTLRGAKTRDTSKWT
                     EYLNETLDMWGNDAEVLFMPHTWPVWGNKHINDYIGKYRDTIKYIHDQTLHLANQGYT
                     MNEIGDMIKLPPALANNWASRGYYGSVSHNARAVYNFYLGYYDGNPANLHPYGQVEMG
                     KRYVQALGGSARVINLAQEANKQGDYRWSAELLKQVIAANPGDQVAKNLQANNFEQLG
                     YQAESATWRGFYLTGAKELREGVHKFSHGTTGSPDTIRGMSVEMLFDFMSVRLDSAKA
                     AGKNISLNFNMSNGDNLNLTLNDSVLNYRKTLQSQADASFYISREDLHAVLTGQAKMA
                     DLVKAKKAKIIGNGAKLEEIIACLDNFDLWVNIVTP"
BASE COUNT          574 a          573 c          522 g          484 t
ORIGIN      
        1 aataaattcg cgtaattttc ccccttcagc acaaatgcct aataaaatgg atcgttccgc
       61 tcacacttcc acttattaaa gtcgcaaatt cccaattatg ataaccccag ctttaaatga
      121 cgtattttgc atggattaca tatttacgac ataaatgata aatggagatt tatatgaata
      181 actctcggtt attccgtttg agcagaattg ttattgcgtt aactgccgcc agcggcatga
      241 tggtaaatac cgcttacgca acagatgaag cgaaagccgc cactcaatat acccaacagg
      301 ttaatcagaa ttacgccaaa tcattaccgt ttagcgatcg tcaggatttt gacgacgctc
      361 agcgtggatt tatcgctccg ctgctggatg aaggtattct gcgcgatgct aatggcaaac
      421 catattatcg cggggaagat tataaatttg atatcaatgc gcccgcgccc gaaaccgtta
      481 accctagcct gtggcgtcag tcgcagttaa acggtatttc cggcctgttt aaagtcaccg
      541 acagaatgta ccaggttcgc ggtcaggata tctccaacat cacctttatc gaaggtgata
      601 ccgggattat tgtcatcgac ccgctggtga cgccaccgag cgcaaaagcc gcccttgacc
      661 tttacttcca aaatcgcccg caaaaaccga ttgttgcggt tatttatacc cacagccata
      721 ccgaccacta tggcggcgtg aaaggcatta tctctgaagc cgatgtgaaa tccggtaaag
      781 tgcaggttat cgcccctgct ggctttatgg acgaagccat cagcgaaaac gtactggcgg
      841 gtaatattat gagccgccgt gcactttact cctacggcct gctgctggcg cataaccctc
      901 agggtaacat cggcaatggt cttggcgtaa cgctggcatc gggctacccg agcatcatcg
      961 caccgaacaa aaccatcacc aaaaccggtg agaagatgat tatcgacggc ctggagtttg
     1021 acttcctgat gaccccaggt agcgaagcgc cagccgaaat gcacttctat attccggccc
     1081 tgaaagccct gtgtaccgcc gagaacgcca cgcataccct gcacaacttc tacactctgc
     1141 gcggcgcgaa aacccgcgac accagcaagt ggaccgagta tctgaacgaa acgctggata
     1201 tgtggggtaa cgacgcggaa gtcctgttta tgccgcacac ctggccggtc tggggcaata
     1261 agcatatcaa tgattatatt ggtaaatatc gcgatactat caagtacatt cacgaccaga
     1321 ccctgcacct ggcgaaccag ggctacacca tgaatgaaat cggcgacatg attaaactgc
     1381 cgcctgcact tgccaataac tgggccagcc gtggctatta cggttctgtc agccacaacg
     1441 cccgcgcggt gtataacttc tatcttggct attacgacgg taacccggct aacctgcatc
     1501 cgtatggtca ggtggagatg ggtaaacgtt acgtgcaggc gctgggcggt tctgcccgtg
     1561 tcatcaacct ggcgcaagaa gcgaacaagc aaggtgatta ccgctggtcg gcagaactgc
     1621 tgaaacaggt gattgccgcc aacccgggtg accaggtcgc gaagaatctg caagcgaata
     1681 actttgaaca gctgggctat caggccgagt ccgccacctg gcgcggtttc tacctgaccg
     1741 gcgcgaaaga gctgcgcgaa ggggtgcata agttcagcca cggcaccacc ggttccccgg
     1801 acaccattcg cgggatgtcg gtcgaaatgc tgttcgactt tatgtccgtt cgcctcgata
     1861 gcgcgaaagc cgcgggtaaa aatatcagcc tgaacttcaa tatgagcaat ggcgataacc
     1921 tcaacctgac gctgaacgat agcgtgctta actaccgtaa aacactgcaa tcccaagctg
     1981 acgcctcttt ctacatcagc cgtgaagatc tgcacgccgt gctgaccggg caagccaaaa
     2041 tggcggatct ggtaaaagcg aagaaagcca aaattattgg caatggcgcg aaactggaag
     2101 aaattatcgc ctgtctggat aatttcgatt tgtgggtgaa tatcgtaacc cca
//
LOCUS       DAZFQD010000286         2116 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.291, whole genome shotgun sequence.
ACCESSION   DAZFQD010000286 DAZFQD010000000
VERSION     DAZFQD010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2116
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.291"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(1..1585)
                     /locus_tag="ACHKTW_005205"
                     /pseudo
     CDS             complement(1..1585)
                     /locus_tag="ACHKTW_005205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309571.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
     gene            complement(1531..1752)
                     /locus_tag="ACHKTW_005206"
     CDS             complement(1531..1752)
                     /locus_tag="ACHKTW_005206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6466051.1"
                     /translation="MAKIRYLHGTHDARAGDIRDVAQPCAEVLVRLGKAEYITVRRPA
                     GQKKKRDAEHGECGTFYGEPEKTRNQDVT"
     gene            complement(1797..>2116)
                     /locus_tag="ACHKTW_005207"
     CDS             complement(1797..>2116)
                     /locus_tag="ACHKTW_005207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFO6466052.1"
                     /translation="LGGSFQGLPVIVSQYVGDQLVLVNAPDIYLADDGGVAVDMSREA
                     SLEMQSEPTGDSTTPSPVELVSMFQTGSVAIRAERWINWRRRRTAAVAVITGVNYGSA
                     SGG"
BASE COUNT          427 a          708 c          516 g          465 t
ORIGIN      
        1 accggaatgg cccccacagc ctcatccacc atcgcctgca gaaccggacg cacctcatcc
       61 accgtcacat gcttctgtaa taccgccgac agggaagtca gtttctcttc aaacgcttgt
      121 gcctgcgagg ccatcttccc ctcaaatgtg cgctgtaaat ccgccagcac tgtggagaat
      181 tcttcaccca gcgcacggat aatggacagt tcacgctctg tcattttcgc agtatccccc
      241 tgaacatcgc cttcaccgca tcacgctctg tttcgcttat ggccttatta ccgtcagacg
      301 cgccctcctg gcgtgtgcct gacgacgttt tcccggaaga cgcgaacgga tcctcacggg
      361 catcacgacg ggacagcgcc tccagactgt agttctgctg ctgaagatac agtgcatcgc
      421 ctcctgccag gggcggcaga ttctcacgtt tacgggcctc attgggcgtg agaagcgtat
      481 ttttcaccgc atcccccagc gttttcatgc gacgttcgct gtccattctc agcagcgtgg
      541 tgacgtcaaa ctccgtgctc tcgttttccc ctgtttccag cgcctcatcc agtaacagct
      601 caatggactc aatcagcgtc tgcagacact gggaataata ctgctgctcc agcgcctcca
      661 cgttgtcact ggaaggcggt tgtcccacac caatcttgta ggccgggaca cggaacaccg
      721 aacagacaat ttcagccgtc attttcagct gttccaccgt ctgcgcatcc accggtgaaa
      781 acgtcgtggg gttatatttc gccccgttgc tcaggatcgc cgttttcccc gcattttcgc
      841 cggtataccc gctgtcccag ttgctcttca gttttttcgc attttcttcc gttatactgc
      901 cggggatctc aatcaccccg gacggcctgc cgccatttct gaaaaaagac gttgaatttg
      961 cctgaatatg atgcccctgc gtggcagcca gcccggcagc atacaccggc ggcagcccca
     1021 caagcggatg aaaaaaacag ttaaaccggt cgtggatcac ttccctggca ggcaccgtca
     1081 ccgcctccgt gatcccgcag ttccggtccg gtgtaatgcg atagaacacc tcgccgtcat
     1141 ccgccaccag aggttcaacc cggctccagt ccagaatacg cagttctttg atctgccccc
     1201 gggagttacg gattttcagc accaccgtat tgccgtgacg cagtttggcg ttcagccaca
     1261 gttcaaaaaa ctggatacga ttctgctgtg cattgggacg acgacagaga cgggcaatat
     1321 ccccccggcg cgtttccctg cgtatcccat gcgcatccgt ctgcataaga cgcagccgca
     1381 ttttggcgat atcctgggat atcagcgaaa tacatgcaaa caccgcatga aaggagagga
     1441 cggcttcagg atcggctttc acgccctgct gccaggcgcc ggaaaagggc tcagccaccg
     1501 cctgaaacag gctggtccag cccgcctctc ttacgtcacg tcctgatttc tggttttttc
     1561 gggttcgccg taaaaggttc cacattcgcc atgctccgca tcacgtttct ttttctgacc
     1621 tgccggacgt cgcaccgtga tgtactccgc ctttcccagg cgaaccagca cctccgcaca
     1681 cggctgtgcc acatcacgga tatccccggc ccgggcatca tgcgtaccat gcagatatcg
     1741 gatctttgcc ataacctgtt acgggaggcg cacacctccc gtcctcctta tcagactcag
     1801 ccgccggacg cactgccgta gttcactccg gtgatcaccg ccaccgccgc ggtacggcga
     1861 cgacgccagt tgatccagcg ctccgcacgg atggccacgc tgcctgtctg gaacatggaa
     1921 accagctcca ccggcgacgg cgtggtactg tcgccggtcg gctcagactg catttccagt
     1981 gatgcctcgc gggacatatc cactgccacg ccgccgtcat ccgccagata aatatccggg
     2041 gcattcacca gcaccagctg gtcacccacg tactgggaga caatcaccgg cagcccctgg
     2101 aaggagccac ccagca
//
LOCUS       DAZFQD010000287         2116 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.292, whole genome shotgun sequence.
ACCESSION   DAZFQD010000287 DAZFQD010000000
VERSION     DAZFQD010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2116
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.292"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..775
                     /locus_tag="ACHKTW_005208"
                     /pseudo
     CDS             1..775
                     /locus_tag="ACHKTW_005208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000961347.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1121..1279
                     /locus_tag="ACHKTW_005209"
                     /pseudo
     CDS             1121..1279
                     /locus_tag="ACHKTW_005209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016239934.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            1503..1622
                     /locus_tag="ACHKTW_005210"
     CDS             1503..1622
                     /locus_tag="ACHKTW_005210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HFO6466053.1"
                     /translation="MPHSYAEYHHFIMMSLMSGEHNTLALSLQDESPGLVPVG"
     gene            1687..>2116
                     /locus_tag="ACHKTW_005211"
     CDS             1687..>2116
                     /locus_tag="ACHKTW_005211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001571801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HFO6466054.1"
                     /translation="MNRTSPYYCRRSVLSLLISALIYAPPGMTAFTPDVIGVVNDETV
                     DGSQRVDERGTTNNTHIINHGQQNVHGGVSNGSLIESGGYQDVGSHNNFVGQANNTTI
                     NGGRQTIHDGGISTGTIIDSGNQDVYTGGISNGTTIKGGNS"
BASE COUNT          636 a          377 c          467 g          636 t
ORIGIN      
        1 ttcatatgaa aaaaatagat gagatttcat ctttgtcaga gtcaaaggaa caccccatag
       61 atattcaaga aaaaaagatg cgtttgtgaa tgagttcaag gggatattat ttgataaaaa
      121 tggaaggtct tcagagtttc tacttaattt ttatgaatgt tgctatgagt ttttaccaag
      181 agcgcagcct caggataaaa tcgaaagcta taattcagca ctgcaagctt tctccatctt
      241 ttgttcatct acgttgatac ataataatat aggctttgat ttcaaattat ttccagaagt
      301 caaagtgtgt ggggaacatc ttgaaacggt attcaaatat aaaaatggcg atgatgtccg
      361 ggagatagcc aaaattaaca ttactctcca aaaagaagag gatggtttat ataatttagg
      421 tggattggat tttaagggat gcttcttttc tggacagaac ttcagtaact atgatattca
      481 atatgtgaac tggggaacgt cattgtttga tcttgatact ccgtgtattt ttaatgcgcc
      541 tgcttacaac aagagtaatg aaaaatcatt aaaacctgtg agcgaaaacg gtttaagtgg
      601 agtcttgact gatcgtaata ataaaataaa actcatcacg ggcgtggcac cattcgatga
      661 tattttattt atggatgatg actttgatga tagttcctct gaggatgatc ccgttgagaa
      721 tagtcctgtt gtgactagtc ccgttgtatc aagttctaaa agcagttttc aatgattaat
      781 aaatggctat ttttacaaag agataataac gtagattgaa acaatatgta ttattgattg
      841 agtaaacggg attattcttc acactacatc cattttactg tcactaccaa agaaaaaggg
      901 gctacgtttt catgtaaccc cttgatttat ttggtgggct ggcagtgact gaattggtgc
      961 tgtaattggt tgaaatattt catctaatgt ctggttcaac tttccttagc tgcctgaaag
     1021 ttttttgatc ttacccagca ataacagcgt tacggtgcac cacgcctgac tgaagaactg
     1081 cgtgctcagg gttaccactt caacgtaaaa ccgtggcagg aggtccggac tgaaaaaact
     1141 ggtcaggaca tcatcggcag ggaacgcact gctagtctgt aaactgaacc gactggggcg
     1201 cagtatgtgg catcttgtcg tgctgttgga ggagctgtgc gaacgtggta ttaactttcg
     1261 tgctctggcc caatctatat ttgcccaaca atggggggac gaatgctgta aaagtaaaac
     1321 aatctgcgat ctcaaagtta ttgtgtgatt tttatgtgag cagaagatat ttatcagaaa
     1381 cgattatgta aatcatttta ttttgccaac ggcctcattg tcgaaagaaa agaatacgcc
     1441 cgtatttcct gaatggattg acttttttat ccaaccacac ttcagcgcac tgcgtttaaa
     1501 aaatgcctca ttcttatgcg gaatatcatc atttcatcat gatgtctttg atgagcggtg
     1561 aacacaatac acttgcgctg tctcttcagg atgaatcccc tggtctggtg cctgtgggct
     1621 gatgttgcag caaagctaag gtcgcttaaa cgctactcag tattcacttt tcagaggatg
     1681 aaatttatga acaggaccag tccctattat tgtcgccgct cagtactttc cttattgata
     1741 tctgccttga tatatgcccc gcctgggatg acggccttca ctcctgatgt tattggtgtg
     1801 gtaaacgatg agactgtaga tggcagccaa agagtagatg aacgaggtac aacaaataac
     1861 actcatatta tcaaccatgg ccagcagaat gttcatggcg gggtatctaa tggaagtctt
     1921 attgaatcgg gtggatatca agatgtagga agtcataaca attttgtggg gcaggctaat
     1981 aatacaacca ttaacggagg aagacagaca attcatgacg ggggcatttc caccggtacg
     2041 ataatcgaca gtggcaatca ggacgtttat acaggtggta tcagcaatgg aacgacaatt
     2101 aagggtggta attcac
//
LOCUS       DAZFQD010000288         2034 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.293, whole genome shotgun sequence.
ACCESSION   DAZFQD010000288 DAZFQD010000000
VERSION     DAZFQD010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2034
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.293"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            117..278
                     /gene="rhuM"
                     /locus_tag="ACHKTW_005212"
                     /pseudo
     CDS             117..278
                     /gene="rhuM"
                     /locus_tag="ACHKTW_005212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774140.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RhuM family protein"
     gene            675..1859
                     /gene="setC"
                     /locus_tag="ACHKTW_005213"
     CDS             675..1859
                     /gene="setC"
                     /locus_tag="ACHKTW_005213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter"
                     /protein_id="HFO6466055.1"
                     /translation="MQKTATTPSKILDLTAAAFLLVAFLTGIAGALQTPTLSIFLADE
                     LKARPIMVGFFFTGSAIMGILVSQFLARHSDKQGDRKLLILLCCLFGVLACTLFAWNR
                     NYFILLSTGVLLSSFASTANPQMFALAREHADRTGRETVMFSTFLRAQISLAWVIGPP
                     LAYELAMGFSFKVMYLTAAIAFVVCGLIVWLFLPSIQRNIPVVTQPVEILPSTHRKRD
                     TRLLFVVCSMMWAANNLYMINMPLFIIDELHLTDKLAGEMIGIAAGLEIPMMLIAGYY
                     MKRIGKRLLMLIAIVSGMCFYASVLMATTPAVELELQILNAIFLGILCGIGMLYFQDL
                     MPEKIGSATTLYANTSRVGWIIAGSVDGIMVEIWSYHALFWLAIGMLGIAMICLLFIK
                     DI"
     gene            1970..>2034
                     /locus_tag="ACHKTW_005214"
     CDS             1970..>2034
                     /locus_tag="ACHKTW_005214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6466056.1"
                     /translation="MGSTRKGMLNVLIAAVLWGSSG"
BASE COUNT          507 a          465 c          427 g          635 t
ORIGIN      
        1 atattaatac tcatattcga ctcctgtgat ctccgccaaa atgcctctcc tgacgttctc
       61 caaccccttg taatactgtc aatccagata ttttctactg ctcaatattc aatcggcttc
      121 aggaatacct ggtctaaggt tttgtgatgg acgatgagcg gctgaagaat ccgcctgtcg
      181 gttcttctgt cctcgaacgc atccgcgata ttcacgccag tatgcgccgt gtttatttgc
      241 aggtgatttg cccccctgct gactctcagc caactgtatc gccattaagg tgaagatgtt
      301 gttttactcg ccccagaaca ccctttttta tacgactatt ctatttcttt aaccgctggc
      361 gaaaaaaact gactataaac ttcaccaata atcagactgc catcaagatt aatttgttca
      421 tttaatatat caaatcaact tgttttattt ttactatcaa aaatagctca ttgccttact
      481 ataattaata tataaattcg gtaattaatt cttaacatgc ttttactaat aatctcaatt
      541 gctggcccta taatattgcg ctagcattgc tttctggttg tatcagcgat actcaaaaat
      601 tcttaataca atactcattc gactggtact tatttgtaac tcagttatat tttttcgccc
      661 ggtgattcag aagaatgcaa aaaacggcta ccactccatc aaaaatactt gatctcactg
      721 ccgcggcatt tttacttgtc gcctttctga cgggtattgc gggcgctctt cagactccta
      781 ccctaagtat attcctcgca gatgaactga aagcccgtcc tataatggta ggttttttct
      841 tcaccggtag cgctattatg ggaattctgg tcagtcaatt tctggcaagg cactccgata
      901 aacaaggcga ccgtaaatta ctgattctgc tatgttgctt atttggagtg ctggcctgca
      961 cgctttttgc gtggaatcgc aactacttca ttctcctctc aacgggcgta cttctgagta
     1021 gttttgcttc caccgcaaac ccgcaaatgt tcgccctcgc ccgtgaacac gccgacagaa
     1081 caggccgtga gacggtcatg ttcagtacat ttttacgtgc tcagatctcg cttgcctggg
     1141 ttatcgggcc accgctcgct tatgaactgg caatgggatt tagttttaaa gtgatgtatc
     1201 tcaccgcagc catcgcattt gttgtttgcg gactgatagt ctggttgttt ttgccatcaa
     1261 tacaaagaaa tattcctgtc gttacccaac ccgtagaaat tttaccctcc acccacagga
     1321 agcgggatac gcggctactt tttgtggtct gttcaatgat gtgggcggcg aataatctct
     1381 acatgataaa tatgccgcta tttattattg atgaactgca tctaaccgat aaactggctg
     1441 gagaaatgat tggtatcgct gccggtctgg aaattccgat gatgttaatc gcaggctatt
     1501 acatgaaacg tattggcaag cgactattaa tgctcattgc tatcgtgagt ggaatgtgtt
     1561 tttacgccag cgtactcatg gcgacgactc cggcggttga gctggaattg caaattctta
     1621 atgccatctt ccttggtatt ctctgtggta tcggcatgct ttattttcag gacttgatgc
     1681 ctgaaaaaat aggctctgcg acaacgttat atgcaaatac ttcacgcgtc ggctggatta
     1741 tcgccggctc tgttgacgga attatggttg aaatctggag ctaccatgcg ttgttctggc
     1801 tggcgatagg gatgttgggt attgcgatga tttgcctgct gtttattaaa gatatttagc
     1861 tcaccagtct gggctaagaa aaatcggcgt ggcgcaaaca gagccatcct cgccattttt
     1921 gctatcatgc ctgcatacat aaacgacaaa acagtatgca gagggaaaaa tgggttccac
     1981 cagaaagggg atgctgaacg ttctgattgc cgccgtgttg tggggaagtt cagg
//
LOCUS       DAZFQD010000289         1987 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.294, whole genome shotgun sequence.
ACCESSION   DAZFQD010000289 DAZFQD010000000
VERSION     DAZFQD010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1987
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.294"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(175..300)
                     /locus_tag="ACHKTW_005215"
     CDS             complement(175..300)
                     /locus_tag="ACHKTW_005215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6466057.1"
                     /translation="MSIFLSWVILIISVLMAIGIMRIIHTLKDIELFFTGDKRRK"
     gene            complement(297..539)
                     /locus_tag="ACHKTW_005216"
     CDS             complement(297..539)
                     /locus_tag="ACHKTW_005216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4754 family protein"
                     /protein_id="HFO6466058.1"
                     /translation="MIEAYKILINNALQRYHFELANRGVTKDESLRNSLRGAIMGLYN
                     CALGSDDREALEELKSIMTELFEGEIVEPYFTGVAA"
     gene            complement(558..902)
                     /locus_tag="ACHKTW_005217"
     CDS             complement(558..902)
                     /locus_tag="ACHKTW_005217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4761 family protein"
                     /protein_id="HFO6466059.1"
                     /translation="MKKPGNFSQQNFRTSAERHVARQHFRKNSATTTTEIHATPDGHP
                     VKQTGKHTWTIGDTGIVIHKSFRSPVTGRFNFTLTRGDDYFGQDFTFFEALRTADRLI
                     SGLRFQYPGSKH"
     gene            complement(1055..1318)
                     /locus_tag="ACHKTW_005218"
     CDS             complement(1055..1318)
                     /locus_tag="ACHKTW_005218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001031145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6466060.1"
                     /translation="MNKEKSPDYFVKRLQDATESMLIALNNYSYEEAVTVLEWIKEKI
                     TEDSIVKMQSINISRDKTFPLRIPPKRRLVYSIPVSMQCKLPK"
     gene            1364..1774
                     /locus_tag="ACHKTW_005219"
     CDS             1364..1774
                     /locus_tag="ACHKTW_005219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6466061.1"
                     /translation="MRAAERELARSEGFHSFVDARAHAELFRIGARSPVILRSVKKCA
                     QSLVNDKYHLVFLVTESTSGFCLSLSYPENIHLYDEVFESFEGAESFAQHLSYDVFEL
                     ADIYDCWGSLIHPLQHSRFYRNVFDIDDDHPSAW"
     gene            complement(1859..>1987)
                     /locus_tag="ACHKTW_005220"
     CDS             complement(1859..>1987)
                     /locus_tag="ACHKTW_005220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO6466062.1"
                     /translation="YDRQKRKFSPANKEDCQTINGKRVYTGRKSKLEQHQYSGGNQ"
BASE COUNT          432 a          434 c          449 g          672 t
ORIGIN      
        1 atgaatgcct taattaattg ctcgtgtgtt ttctggtgtt attccgcgcc tgctttcggc
       61 ttcaggttat tcccagatct tcagggctaa ccagataagc gcactcacct gaaatatcac
      121 ccatacagtg gcgatggtgt gtattgcttt tacattcttc ataaggtttt cgctttattt
      181 acggcgttta tcgccggtaa agaataattc gatatccttc agtgtgtgga taattcgcat
      241 aatgccgatt gccataagca cggagataat cagaattacc catgaaagaa atatgctcat
      301 gcggctaccc ctgtgaagta aggctcaaca atttcacctt caaacagttc tgtcataatc
      361 gactttaatt cttccagcgc ttcacggtca tcgctgccca gtgcgcagtt gtatagcccc
      421 ataattgcgc cgcgcagtga gttacgaagg ctttcatcct tagttacgcc ccggtttgca
      481 agttcgaagt ggtaacgctg caacgcgtta ttaataagta ttttgtaggc ttcgatcatg
      541 gctgaatcct ttatttatca gtgcttgctt cccggatatt gaaaacgcag gccgctgatt
      601 aatctgtctg ccgttcttaa ggcttcaaaa aatgtgaagt cctgtccgaa gtaatcatcc
      661 ccgcgagtca gggtgaaatt aaatctgcca gtaacagggc tgcgaaaact tttgtgaata
      721 acgatgccag tgtcgccaat cgtccaggtg tgcttgccgg tttgttttac cgggtgaccg
      781 tctggtgtgg catggatttc agtggttgtg gtcgcactat ttttgcggaa gtgctggcgt
      841 gcaacgtggc gttcggcact tgttcgaaaa ttctgctgtg aaaaatttcc cggtttcttc
      901 ataaaaatcc ctactgctgc tgtgaatttg tgccgggttt ttagccatgc ccggcgcatg
      961 gttttgtggt aatttcgtta cgccttgcaa ttcttgcccg aactgttgta acgataacca
     1021 accacacaaa ggagtattta tgttttctca taaactattt cggcaatttg cactgcattg
     1081 agactggtat cgagtaaacc agtcgtcgtt ttggcggtat tcgcagtgga aatgttttgt
     1141 cacgggagat gttgatactc tgcatcttga cgatgctgtc ttccgttatt ttttccttta
     1201 tccactcaag taccgttacg gcctcttcgt aagagtaatt attcagggca atcaacattg
     1261 actctgttgc atcctgtaat cgtttcacaa aataatccgg agatttctct ttattcatat
     1321 gaaaattatc cttacatccg aatctgggcg gtggttttgg gttttgcgtg ctgccgaacg
     1381 tgagcttgca agaagtgagg ggtttcacag ttttgttgat gcccgtgctc atgcagaact
     1441 ctttcgtatt ggcgcgcgtt cccctgtgat tcttcgctct gtaaaaaaat gtgctcagtc
     1501 actggtgaat gataaatatc accttgtctt cttggttacg gaatctacgt ctggtttttg
     1561 cttgtcactt tcgtatcctg aaaacattca cttgtatgat gaggtgtttg agtcttttga
     1621 aggtgccgaa tcgtttgctc aacatctctc atacgatgtg ttcgaactgg ctgatattta
     1681 tgattgttgg gggtcgctta ttcatccctt gcaacattct cgtttttaca gaaatgtgtt
     1741 tgatattgat gatgatcatc catcagcctg gtaggctgct tgcataacaa ccattttcca
     1801 cagcatctgc ggcgaaatct cattcctggg atttcgctgt tgccggtttg ttttgatgtc
     1861 actggttgcc tccactgtat tggtgttgct ccagttttga ctttcttcct gtgtaaaccc
     1921 gctttccatt gatggtctgg cagtcttctt tgtttgccgg ggaaaacttt cttttctgtc
     1981 ggtcgta
//
LOCUS       DAZFQD010000290         1965 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.295, whole genome shotgun sequence.
ACCESSION   DAZFQD010000290 DAZFQD010000000
VERSION     DAZFQD010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1965
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.295"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..1965
                     /locus_tag="ACHKTW_005221"
                     /pseudo
     CDS             1..1965
                     /locus_tag="ACHKTW_005221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
BASE COUNT          683 a          383 c          370 g          529 t
ORIGIN      
        1 ctccagatac aacttatcga gagttgaata aagcagcaca gcgtagcatt acagcgccag
       61 acaggatagg agaaggaaaa tcatgggttt atcaacgaga tacaatgatt gaaagaggtg
      121 ataacagcgg tgttcatcag tatggtcctg ctgaacattt cacccacatt atatctgaca
      181 aaccgtcccc aaaagatgag tatattgcat atgctattaa cattcctgac tatgagctgg
      241 cagccgatgt ataaatatta acgtgacgtc accttccgaa cagcaagaaa catttaaaat
      301 actaatcaat ccagaacatc tacggcaaac acttgagcgt aaatctctta ctgctgttca
      361 gaaatcacaa tgtgaaatca taacccccaa aaaacctggc gaagcgattc ttcatgcttt
      421 taatgccacc taccagcaaa tcagggaaaa tatgtctgag tttgcacgtt gccattatgg
      481 gtatatacaa atccctccag tgacaacttt ccgcgccgac ggaccagaaa ctcccgaaga
      541 agaaaagggt tactggtttc atgcttatca acccgaagat ctttgtacca tccataatcc
      601 aatgggagat ttgcaggatt ttattgcatt ggttaaagat gctaaaaaat ttggtatcga
      661 tatcattcct gattatacct ttaactttat gggaattggg ggtagtggta aaaatgacct
      721 ggattatccc tctgctgata tacgagcgaa gatcagtaaa gatatagaag gtggtatccc
      781 tagctattgg caaggtcagg ttttgattcc attcattaaa gatccagtaa caaaagaacg
      841 taaacaaatc catccagaag atatacatct cactgcaaaa gacttcgaag caagtaaaga
      901 taacatctct aaggatgaat gggaaaacct ccatgcatta aaagaaaagc gtttaaatgg
      961 aatgcctaaa acaacaccca aaagtgacca ggttattatg ttgcaaaatc aatacgttcg
     1021 tgaaatgcga aaatatggcg tacgaggttt acgttatgat gcggcaaaac actcaaaaca
     1081 tgaacaaata gaaagatcaa taaccccacc gcttaaaaat tataatgagc ggttacacaa
     1141 tactaactta tttaacccaa aatatcataa aaaagccgtt atgaattaca tggaatatct
     1201 ggtaacttgt cagttggatg aacaacaaat gtcatcactg ctttatgaaa gagatgattt
     1261 aagcgccatt gatttttcat tgctcatgaa aacgataaaa gccttttcat ttggtggaga
     1321 tcttcaaacc cttgcatcaa aaccgggttc cacaatctca agtatcccat cagaaagacg
     1381 gatattgatt aacattaacc acgattttcc taacaatggt aatcttttca atgactttct
     1441 atttaaccat caacaagatg aacaattagc aatggcatat atggccgctc tgccgttcag
     1501 caggccttta gtttactggg atggctaagt attaaaatca acgactgaaa ttaaaaatta
     1561 tgatgggtcc acgcgtgttg gcggtgaggc gtggcttaat aaaggttgct ctacctatca
     1621 gcagctctac aatgaattcc acgcattata tatagataaa gcaggaatat ggagcgcatt
     1681 tgagggtata tttgcaacta aaaacgttct ggcctttagt cgtggggatt ctgtgaacat
     1741 taatcactct cctcatgatg gactagttat aataaataaa ggaaacgaag aagttgaagg
     1801 tacctggcct aacaaattgc aacctggaat atataaaaac atggggagta atagcgttaa
     1861 cattattatt aataataccc aaaaaattat ccccccctag taaagtattt acgcttagag
     1921 gcggatctct aaatatcaat attcctgggc gtagcgctct tcttt
//
LOCUS       DAZFQD010000291         1940 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.296, whole genome shotgun sequence.
ACCESSION   DAZFQD010000291 DAZFQD010000000
VERSION     DAZFQD010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1940
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.296"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            33..221
                     /locus_tag="ACHKTW_005222"
     CDS             33..221
                     /locus_tag="ACHKTW_005222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001306377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6466063.1"
                     /translation="MKWFTPEHVISAFKKGELTRHQVVMNRNMARSRGYPERAACFNE
                     ALKIIDELRKNEKESETE"
     gene            460..1131
                     /locus_tag="ACHKTW_005223"
     CDS             460..1131
                     /locus_tag="ACHKTW_005223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO6466064.1"
                     /translation="MIKHTKPFLGIDYDLLAITSLVINGKTKEVKLHHKIIYSYLLSL
                     SGTYQKVTPSLKGIGERLGVGTDQTVRRHIKDLVSFGWLKIEERAGSTNAYIVLPYEQ
                     EAKTQQEATVKAVPEQLPPTEDNKESSFDVLDDWDAPLPWETEETPVSPIFDEKVTND
                     REDVLENFASLICQEKNTRTGGASFIEFANSLAYRYGLKLPNGIEAYFAKKHPKVYED
                     FDLPF"
     gene            1148..1426
                     /locus_tag="ACHKTW_005224"
     CDS             1148..1426
                     /locus_tag="ACHKTW_005224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000617150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6466065.1"
                     /translation="MIQYLIKSKVDRIQCNDTGKRIYETLAYLYKGKPTPLKYSDVLH
                     RAACSEDGLKFWLKQLSNFGVIEIKELSFSTFNLKRLDKEIEFIYSTL"
     gene            1559..>1940
                     /locus_tag="ACHKTW_005225"
     CDS             1559..>1940
                     /locus_tag="ACHKTW_005225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001129164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6466066.1"
                     /translation="MAKRKNNVVKKIGDSASLLSKPKSILRRKDFKELVTLTKQNSAP
                     GEWKTEIIEHSPSVPCGDEYIALQEILSSTPGVFWKPRKRKEYIVDSSDLRKYQILGF
                     EDYNHYVGYLATNGLNNLVPEFQIL"
BASE COUNT          689 a          328 c          373 g          550 t
ORIGIN      
        1 gttttttctt ttaactgaat aaggagtttt ttatgaaatg gtttaccccg gagcacgtca
       61 tctcggcatt taaaaaaggt gagctaacac gccaccaagt agtaatgaac cgtaatatgg
      121 caaggagtcg aggttatcca gagcgagctg cttgcttcaa tgaagcactt aagattattg
      181 atgaattaag aaaaaatgaa aaagaatctg aaacagagta aagaagagaa ggagaaaact
      241 atgagcgaaa ctacagaaaa gacaaccacg cacttgcttt catattcctg atgaagtatt
      301 tcaggtgtta tttaaagaag ccagaagtag acggatcact gtcacagatt acattcttga
      361 tatgttgaaa agagaggctg aaacactctc aaataaaagc aagtgatttt tatcaccata
      421 gtagcgccat agagcgtgat tacatcaagg agatttttaa tgattaaaca tacaaaacct
      481 tttttaggaa tagattatga cttactagca atcacttccc tggtgattaa cggcaaaaca
      541 aaagaagtaa aactacacca taagatcatt tattcttatt tgttaagttt atctggcaca
      601 taccaaaagg taacaccatc tttgaaaggt attggtgagc ggttaggagt aggtacagat
      661 cagacggtac gccgtcatat caaagatctt gtttcttttg gttggttgaa gattgaggaa
      721 agggcaggta gcacaaatgc ttatattgtc cttccttatg aacaagaagc caaaacacaa
      781 caagaagcta ccgttaaagc tgttcctgaa cagcttcccc ctacagaaga taataaagag
      841 tcttcttttg atgttcttga tgattgggat gcgcctttgc cgtgggagac ggaagaaaca
      901 cctgtttcac caatatttga tgaaaaggta acgaatgata gggaggatgt gttagaaaat
      961 tttgcctctc ttatctgcca ggagaaaaac accagaacag gaggggcatc atttattgag
     1021 tttgcaaatt cactagctta tagatacggg ctaaagctac caaatggtat tgaggcatat
     1081 tttgctaaga aacatcccaa agtatatgaa gactttgatc ttccctttta ataagtaagg
     1141 aggactattg atacagtatt taataaaaag caaagtagac agaattcagt gtaatgacac
     1201 aggaaaacgc atctacgaaa cgcttgctta cctctataaa ggaaagccaa caccgctaaa
     1261 atatagcgat gtgttacatc gagcagcttg ctctgaagat ggtttaaaat tctggctgaa
     1321 acagctatca aatttcggtg ttatagagat taaagaatta tctttctcta cattcaatct
     1381 caaaagacta gataaagaaa tagaattcat ctattccact ctctaaaatc tctctatgta
     1441 attaaaatat aaaaggaaaa cattattatg atagtagcta tgtattggtt attgttcttt
     1501 ctcacgttag aagcatattt cggggtgttt tcctttaaaa gaaaaggaaa aataaattat
     1561 ggcaaaaaga aaaaacaacg ttgttaagaa gatcggtgat tcagcttctc ttctaagtaa
     1621 acctaaatct attctaagac gtaaagactt taaagagcta gttacgctca ccaagcaaaa
     1681 tagcgcccct ggcgaatgga aaacagagat catagaacat tctccctctg tgccttgtgg
     1741 agatgaatat atcgcattac aagaaatctt atcttcaaca cctggcgtat tctggaaacc
     1801 tagaaaaaga aaagagtata tcgttgatag ctctgattta cgcaaatacc agattttagg
     1861 atttgaagat tataatcact acgttggtta tctcgccacc aatggcctaa ataatttagt
     1921 tcctgaattc caaattttag
//
LOCUS       DAZFQD010000292         1930 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.297, whole genome shotgun sequence.
ACCESSION   DAZFQD010000292 DAZFQD010000000
VERSION     DAZFQD010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1930
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.297"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..157)
                     /gene="ynfU"
                     /locus_tag="ACHKTW_005226"
     CDS             complement(<1..157)
                     /gene="ynfU"
                     /locus_tag="ACHKTW_005226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc-binding protein YnfU"
                     /protein_id="HFO6466067.1"
                     /translation="MSERKDSKSRRNYLVKCSCPNCTQESEHSFSRVQKGALLICPHC
                     NKVFQTNL"
     gene            377..592
                     /gene="essD"
                     /locus_tag="ACHKTW_005227"
     CDS             377..592
                     /gene="essD"
                     /locus_tag="ACHKTW_005227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416074.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage lysis protein EssD"
                     /protein_id="HFO6466068.1"
                     /translation="MKSMDKLTTGVAYGTSAGNAGFWALQLLDKVTPSQWAAIGVLGS
                     LVFGLLTYLTNLYFKIKEDRRKAARGE"
     gene            597..908
                     /gene="ydfR"
                     /locus_tag="ACHKTW_005228"
     CDS             597..908
                     /gene="ydfR"
                     /locus_tag="ACHKTW_005228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1327 domain-containing protein"
                     /protein_id="HFO6466069.1"
                     /translation="MTQDYELVVKGVRNFENKVTVTVALQDKERFDGEIFGLDVAMDR
                     VEGAALEFYEAAARRSVRQVFLEVAEKLSEKVESYLQHQYSFKIENPANKHERPHHKY
                     I"
     gene            905..1438
                     /locus_tag="ACHKTW_005229"
     CDS             905..1438
                     /locus_tag="ACHKTW_005229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO6466070.1"
                     /translation="MNTKIRYGLSAAVLALIGAGASAPQILDQFLDEKEGNHTMAYRD
                     GSGIWTICRGATVVDGKTVFPNMKLSKEKCDQVNAIERDKALAWVERNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRLNAGDRKGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VIRRDQESALTCWGIEQ"
     gene            1435..>1930
                     /gene="rzpQ"
                     /locus_tag="ACHKTW_005230"
     CDS             1435..>1930
                     /gene="rzpQ"
                     /locus_tag="ACHKTW_005230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2514 domain-containing protein"
                     /protein_id="HFO6466071.1"
                     /translation="MNQIFMVIFLVLSGFIVGNVWSDRGWQKKWAERDAAALSQEVNA
                     QFAARIIEQGRTIARDEAVKDAQQKSAEISARAAYLSDSVNQLRAEAKKYAIRLDAAK
                     HTADLAAAVRGKTTKTAEGMLTNMLGDIAAEAQLYAEIADERYIAGVTCQQIYESLRD
                     KKHQM"
BASE COUNT          546 a          384 c          487 g          513 t
ORIGIN      
        1 taagatttgt ctggaatact ttgttgcaat gagggcagat caaaagggca cctttttgta
       61 ctcttgaaaa actgtgttct gactcttggg tgcagtttgg gcaggaacat ttaacgagat
      121 aattacggcg tgattttgag tctttacgtt ctgacatagg cttttcctgt ataaatggcc
      181 gtatacagta cactaaatat gaaaacattt ctcgtattat tattttatat atgactttct
      241 ttcaaaataa ttactcacat ttttaatgtg tatgtttctt tagcgccgtt gagaacaacg
      301 tgtgctgtca aaactacccc gtagactccg atcttttcaa acatattgca ccatccgtgt
      361 acatcggggt gaggatatga aatcaatgga taagttaaca acaggtgttg cctatggcac
      421 atcggcgggt aatgctggtt tctgggcatt gcagttactc gataaagtaa ctccgtcaca
      481 gtgggctgca atcggtgtgc tgggtagcct ggtttttggc ctgctgacgt atctgacaaa
      541 tctttatttc aagattaaag aagacaggcg taaggctgcg agaggagagt aatccaatga
      601 ctcaagacta tgaactggtt gtgaaaggag tccgtaattt tgagaataaa gttacggtaa
      661 ctgtagcctt acaggacaaa gaacgctttg acggtgaaat ttttggcctg gatgtcgcca
      721 tggaccgtgt tgaaggagct gcgctggagt tttatgaggc agcagccaga aggagcgtcc
      781 ggcaagtctt cctggaagta gcagaaaaat tgtcagaaaa agttgagtct tatctgcagc
      841 atcagtactc ctttaagatt gaaaatcctg ccaataagca cgagcgtcct catcataaat
      901 atatatgaac acaaaaatca gatacggcct gtcggctgcc gttctggcgc tgattggtgc
      961 tggcgcatct gctcctcaga tacttgacca gtttctggac gaaaaagaag gtaaccacac
     1021 aatggcatac cgcgatggtt ctggtatatg gaccatctgt cggggtgcca cagtggtgga
     1081 tggaaaaacc gtttttccca atatgaaact gtcgaaggaa aaatgcgacc aggtcaacgc
     1141 cattgagcgt gataaggcgc tggcatgggt ggagcgcaat attaaagtac cactgaccga
     1201 accacaaaaa gcgggtatcg cgtcattttg tccctataac attggtcccg gtaagtgttt
     1261 cccgtcgacg ttttataagc ggctgaatgc tggtgatcgt aaaggtgcat gcgaagcgat
     1321 tcgctggtgg attaaggatg gcggacgcga ttgccgcatt cgttcaaata actgttacgg
     1381 tcaggttatt cgtcgtgacc aggagagcgc attaacctgc tgggggatag aacagtgaat
     1441 cagatattca tggtgatttt tctcgtgttg tcaggattta tcgtcggaaa tgtctggagc
     1501 gaccgaggat ggcaaaaaaa atgggcggaa cgtgatgctg ccgcattatc acaagaggta
     1561 aatgctcaat ttgctgctcg aataattgaa caggggcgaa ctatagcccg tgatgaggct
     1621 gttaaagatg cgcaacagaa atctgctgaa atttctgcca gggctgctta tctgtctgat
     1681 agtgttaacc agttgcgtgc cgaagcaaaa aaatatgcca tacgccttga cgcagcgaag
     1741 cataccgcag atcttgccgc tgccgtcaga ggcaaaacaa ccaaaaccgc cgaaggaatg
     1801 ctcaccaaca tgctcggaga tattgcagca gaagctcagc tttatgctga aattgctgac
     1861 gaacgctaca tcgcaggagt gacttgtcaa cagatctatg aatctttaag agataaaaag
     1921 catcaaatgt
//
LOCUS       DAZFQD010000293         1889 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.298, whole genome shotgun sequence.
ACCESSION   DAZFQD010000293 DAZFQD010000000
VERSION     DAZFQD010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1889
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.298"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(443..1381)
                     /gene="lrhA"
                     /locus_tag="ACHKTW_005231"
     CDS             complement(443..1381)
                     /gene="lrhA"
                     /locus_tag="ACHKTW_005231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HFO6466072.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPLPLVLLDDPSPFRDMVLATLNKADIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
BASE COUNT          527 a          435 c          406 g          521 t
ORIGIN      
        1 gtttacataa tatcaacacc attagcattt aatgcccttt caccccagat ccttgacgac
       61 tccaggataa ttagatgttg ttgaatcgtg tccgttgtga agcaatggaa aaaatacggg
      121 tctattttga caggaatttg tgtcgattcc tccccccaaa agagagtatt ttcttgatct
      181 gtgacacgct tttgtcattc cataacaaaa acgcagcaac aaatttacgt attttttaac
      241 atcattgtag caggtgattt ttttcaggcg attatttgtg cgttcgggac gtgaatctct
      301 ggtgattgaa aaatgaacag ttttgtacgt tctgcactat gcgaggaagg atttttacta
      361 aaaaaaagcc gctggggttt aaaacacccc cagcggctcg ttttttacac tattgtctca
      421 ggaattatct atcgtccgtc gattactcga tatccctttc aatcaacaat gaatcatccc
      481 cttccggagc agacattggg ctgtattgcc acggattatg gtagctctcc atcgcctgat
      541 agatcacctg ggccagctca ttattactgg acgggtcata gcacagcaaa tattcggtat
      601 caggtagcgg cggtaagcca tctactccac tcagcacacg caggtccggg ctcatcatct
      661 caaccggcct tgccgtcacg ccaagaccgg ctttcactgc cgcacgaacg gccggaagcg
      721 tcgaggcgac ataagccagt cgccatggaa tatctgcttt attaagcgtc gccagcacca
      781 tatcgcgaaa cggacttgga tcatccagca atacaagagg gagcggctcg cctttttgca
      841 atacgtattc cgctgcgcag taccagtgtg ttggcgaggt tcgcaggttc aatgccttaa
      901 acgctgaagg acgatgggtg gttaccatca aatccacttc ctgggattcc agcatttctg
      961 ccatataggc attacgttta acgcggacat ccagcgctaa tttcggataa accgaactca
     1021 cgcgatttaa caggaaaggt aagatcgtat cggcagattc atctgaagca ccgatagtta
     1081 acacgccctg aagattactg aacattaatg atgagcaggc ctcatcatta aaacgcagga
     1141 ttttcctggc gtaaccaaga agttgaatgc catgttcagt taacagtttg ttgcgaccgt
     1201 gacgagcgaa cagttctttc ccaacgagtt gttccagacg ctgcatttgc tgacttacgg
     1261 cggactgagt acgacacaca gcggcagctg cggcagcaaa agtgttcaga tcggcaacag
     1321 caacaaatgt tctcagcaga tcgaggtcga ggttaattat cggacgattt gcacttatca
     1381 tatattatca cttactggcg gctcatactg agctggttaa tgctgtgcac acacaaacaa
     1441 gcaattccat ttgtaatgtg cctccctggc agtttcatcc ggaacccgga cgaaagtaaa
     1501 aatgcatatg agttgcacta aaaaagcgac tcacattgtt ccgttataat gcctgaagta
     1561 gatcacagaa tatatcttca gggatcgcat atctattaag ttactcactc ttttctattt
     1621 atgacatgcg cgtgtttgta taaatgtaaa tgtgagtcct tgttccactc tcgtgcagca
     1681 tcgctggtca tacgcgaaca cgtacaaaca gcaatggtga ggcggcatca agagcaggga
     1741 tccgtcattt atgcgagcat tttaccccaa aaacctttta tttataaggg tcattgcgaa
     1801 ttatctgatg caaagttatg ttatgttagg caaagtaatc ttctattatt aataagcaca
     1861 tcaaaacctt tttgaatatt aaataataa
//
LOCUS       DAZFQD010000294         1888 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.299, whole genome shotgun sequence.
ACCESSION   DAZFQD010000294 DAZFQD010000000
VERSION     DAZFQD010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1888
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.299"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>1888
                     /locus_tag="ACHKTW_005232"
     rRNA            <1..>1888
                     /locus_tag="ACHKTW_005232"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          511 a          417 c          586 g          374 t
ORIGIN      
        1 gcgtctggaa aggcgcgcga tacagggtga cagccccgta cacaaaaatg cacatgctgt
       61 gagctcgatg agtagggcgg gacacgtggt atcctgtctg aatatggggg gaccatcctc
      121 caaggctaaa tactcctgac tgaccgatag tgaaccagta ccgtgaggga aaggcgaaaa
      181 gaaccccggc gaggggagtg aaaaagaacc tgaaaccgtg tacgtacaag cagtgggagc
      241 acgcttaggc gtgtgactgc gtaccttttg tataatgggt cagcgactta tattctgtag
      301 caaggttaac cgaatagggg agccgaaggg aaaccgagtc ttaactgggc gttaagttgc
      361 agggtataga cccgaaaccc ggtgatctag ccatgggcag gttgaaggtt gggtaacact
      421 aactggagga ccgaaccgac taatgttgaa aaattagcgg atgacttgtg gctgggggtg
      481 aaaggccaat caaaccggga gatagctggt tctccccgaa agctatttag gtagcgcctc
      541 gtgaattcat ctccgggggt agagcactgt ttcggcaagg gggtcatccc gacttaccaa
      601 cccgatgcaa actgcgaata ccggagaatg ttatcacggg agacacacgg cgggtgctaa
      661 cgtccgtcgt gaagagggaa acaacccaga ccgccagcta aggtcccaaa gtcatggtta
      721 agtgggaaac gatgtgggaa ggcccagaca gccaggatgt tggcttagaa gcagccatca
      781 tttaaagaaa gcgtaatagc tcactggtcg agtcggcctg cgcggaagat gtaacggggc
      841 taaaccatgc accgaagctg cggcagcgac actgtgtgtt gttgggtagg ggagcgttct
      901 gtaagcctgc gaaggtgtgc tgtgaggcat gctggaggta tcagaagtgc gaatgctgac
      961 ataagtaacg ataaagcggg tgaaaagccc gctcgccgga agaccaaggg ttcctgtcca
     1021 acgttaatcg gggcagggtg agtcgacccc taaggcgagg ccgaaaggcg tagtcgatgg
     1081 gaaacaggtt aatattcctg tacttggtgt tactgcgaag gggggacgga gaaggctatg
     1141 ttggccgggc gacggttgtc ccggtttaag cgtgtaggct ggttttccag gcaaatccgg
     1201 aaaatcaagg ctgaggcgtg atgacgaggc actacggtgc tgaagcaaca aatgccctgc
     1261 ttccaggaaa agcctctaag catcaggtaa catcaaatcg taccccaaac cgacacaggt
     1321 ggtcaggtag agaataccaa ggcgcttgag agaactcggg tgaaggaact aggcaaaatg
     1381 gtgccgtaac ttcgggagaa ggcacgctga tacgtaggtg aagtccctcg cggatggagc
     1441 tgaaatcagt cgaagatacc agctggctgc aactgtttat taaaaacaca gcactgtgca
     1501 aacacgaaag tggacgtata cggtgtgacg cctgcccggt gccggaaggt taattgatgg
     1561 ggtcagcgca agcgaagctc ttgatcgaag ccccggtaaa cggcggccgt aactataacg
     1621 gtcctaaggt agcgaaattc cttgtcgggt aagttccgac ctgcacgaat ggcgtaatga
     1681 tggccaggct gtctccaccc gagactcagt gaaattgaac tcgctgtgaa gatgcagtgt
     1741 acccgcggca agacggaaag accccgtgaa cctttactat agcttgacac tgaacattga
     1801 gccttgatgt gtaggatagg tgggaggctt tgaagtgtgg acgccagtct gcatggagcc
     1861 gaccttgaaa taccaccctt taatgttt
//
LOCUS       DAZFQD010000295         1832 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.300, whole genome shotgun sequence.
ACCESSION   DAZFQD010000295 DAZFQD010000000
VERSION     DAZFQD010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1832
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.300"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..317)
                     /locus_tag="ACHKTW_005233"
     CDS             complement(<1..317)
                     /locus_tag="ACHKTW_005233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly chaperone"
                     /protein_id="HFO6466073.1"
                     /translation="MKKDLKTLALARLSGFRHKTVKVPEWGNVSVVLREPSAEAWYLW
                     QDVLNGDGEDDDTLSVVAKTRRNLEADVTLFCDVLCDTDLQRVFTPDDREQVLAVYGP
                     VHAR"
     gene            complement(332..1048)
                     /locus_tag="ACHKTW_005234"
     CDS             complement(332..1048)
                     /locus_tag="ACHKTW_005234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major tail subunit"
                     /protein_id="HFO6466074.1"
                     /translation="MSALYERSQLTQVMISSAPATAETMDKAEYLRLDCTIKEVQFTA
                     GQKQDIDVTTLCSTEQENINGLGASSEISMSGNFYLNQAQNALRDAYDNDALYAFKVQ
                     FPSGKGFKFLAEVRQHTWSSGTNGVVAATFSLRLKGKPVSFVVPLAFVKNLEKTLTVN
                     TGALLTMSVSANGGMPPYKYAWKKDGQPVDGQTTDTFSKPGAQSADAGKYTCVVTDSA
                     EKAQSVTSVECTVTVSAAAG"
     gene            complement(1115..1459)
                     /locus_tag="ACHKTW_005235"
     CDS             complement(1115..1459)
                     /locus_tag="ACHKTW_005235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3168 domain-containing protein"
                     /protein_id="HFO6466075.1"
                     /translation="MTEADLYPHLAHLAGGQVYPYVVPLLDGRPSVALPWVVFSLISS
                     VSADVMGGQAESSVSVQIDVYAGTVTQARQIRQDAREAIMLLAPGAVSEMQDYIPENR
                     CYRATLEFQVTV"
     gene            complement(1456..>1832)
                     /locus_tag="ACHKTW_005236"
     CDS             complement(1456..>1832)
                     /locus_tag="ACHKTW_005236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HFO6466076.1"
                     /translation="ENNRVLREATRAGANVLKEEVVSRAPVRRGKLRRNVVVLSRRSR
                     DGGMESGVHIRGVNPDTGNSDNTMKADNPRNAFYWRFVEMGTVNMPPHPFVRPAFDVR
                     SEQAAQVAIVRMNWAIDEVLRR"
BASE COUNT          402 a          597 c          446 g          387 t
ORIGIN      
        1 cgggcatgta ccggaccata gacggccagc acctgctcac ggtcgtccgg agtgaacacc
       61 cgctgcaggt ccgtatcaca caggacatcg cagaacagcg tcacatccgc ttccaggtta
      121 cggcgggttt tcgccaccac cgacagggta tcgtcatcct ctccatcacc attgagcacg
      181 tcctgccaca gataccaggc ctctgccgaa ggctcccgca gcaccacgct gacattaccc
      241 cattccggca ccttcaccgt tttatgacga aaccctgaca gtctggccag cgccagcgtt
      301 ttcagatcct ttttcatgat gacccatccc cttatccggc ggctgcgctc actgtcacgg
      361 tgcattcaac agacgtcaca ctctgtgctt tctctgccga atcggtcacc acgcaggtat
      421 atttccccgc atcagcggac tgcgcaccgg gcttactgaa ggtgtctgtc gtctgcccgt
      481 caaccggctg accatccttc ttccaggcgt atttatacgg cggcattccc ccgttggcac
      541 tgactgacat tgtcagcagc gcaccggtat tcacggtaag tgtcttttcc agatttttca
      601 caaacgccag cggtaccaca aaggacaccg gtttgccttt cagacgcagt gagaacgttg
      661 cagccaccac gccgttggta ccggatgacc aggtgtgctg acgcacttcc gccaggaatt
      721 taaagccctt accggacgga aactgcacct taaacgcata caacgcgtca ttgtcataag
      781 catcacgcag ggcgttctgg gcctgattca gataaaaatt acccgacatg gaaatctcgg
      841 acgacgcccc cagaccgttg atgttctcct gctctgtgga gcagagcgtg gtcacatcaa
      901 tatcctgttt ctgaccggcg gtgaactgga cttccttgat ggtgcagtcc aggcgcagat
      961 attccgcctt atccatagtt tcagcagtcg ccggggcaga tgaaatcatc acctgcgtca
     1021 gctgtgagcg ttcatacaaa gcagacattc tgcctcctga taataaaaaa cccgcacgcg
     1081 gcggggtatg ggttttgtgg gaaaaaagaa aaagtcacac cgtgacctga aactccaggg
     1141 ttgcacggta acagcggttt tccggaatat agtcctgcat ttcactgacg gctcccgggg
     1201 ccagcagcat tatggcttca cgggcgtcct gacgtatctg acgcgcctgc gtcacagtcc
     1261 cggcataaac gtctatctgc accgacactg aggactccgc ctgcccgccc atcacgtccg
     1321 cagacaccga tgaaatcagg ctgaaaacca cccacggaag cgccaccgac ggcctgccat
     1381 ccagcagggg gaccacatac gggtacacct gcccgccggc aagatgcgcc agatgaggat
     1441 acaaatccgc ctccgtcatc gtctcagtac ctcatcaatg gcccagttca tccgcacaat
     1501 cgccacctgc gctgcctgtt cactgcgcac atcaaacgcc gggcgcacaa acgggtgcgg
     1561 tggcatattc acggtcccca tttccacaaa ccgccagtag aaagcattcc gcgggttatc
     1621 cgccttcatg gtgttatcgc tgttacctgt gtccggatta acaccacgga tatggacacc
     1681 ggattccatc ccgccatcgc gggagcgccg ggaaaggacc accacattgc ggcgcagttt
     1741 tcccctgcgc accggtgccc gtgacaccac ttcttctttc agcacattcg cacccgcacg
     1801 ggttgcctca cgcagcaccc ggttattttc cg
//
LOCUS       DAZFQD010000296         1818 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.301, whole genome shotgun sequence.
ACCESSION   DAZFQD010000296 DAZFQD010000000
VERSION     DAZFQD010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1818
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.301"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..404
                     /locus_tag="ACHKTW_005237"
     CDS             <1..404
                     /locus_tag="ACHKTW_005237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO6466077.1"
                     /translation="EGKENSTYIDPTGTPTICYGHTGPDVKPGMTLTDEECLELLEKD
                     MKWAFAAIDRRVQVPLTRGQTVALASWIFWAGETNFRNSTLLRLINAGQMPASCKQYI
                     RWIYSKGVKLPGLEARRSADEWLCRYDLPKV"
     gene            517..867
                     /locus_tag="ACHKTW_005238"
     CDS             517..867
                     /locus_tag="ACHKTW_005238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014334045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6466078.1"
                     /translation="MAALGVMSVLAAVVWGMHQKTQRLEDNNHVLVRERDEARLVLAN
                     QQRTLQLISQISEAATNEKQQNIQHSEGQQSVVRRSLAAVPAASAPVPDDVADRVRRA
                     VCEIRACEAGADPR"
     gene            851..1069
                     /locus_tag="ACHKTW_005239"
     CDS             851..1069
                     /locus_tag="ACHKTW_005239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001122253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6466079.1"
                     /translation="MPIPAEWLADCLVPPAPEPFTFGASVTYNLQLLAVIKNCNVDKA
                     SIRRLEARRQHEFTDMAGTPAVPAGKTK"
     gene            1079..1306
                     /locus_tag="ACHKTW_005240"
     CDS             1079..1306
                     /locus_tag="ACHKTW_005240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HFO6466080.1"
                     /translation="MDDSDRAQAVMERGSERALCNRLTRKCRAMDAPGRRVCADCGGE
                     IPAARLVAVPDAIRCVNCQNIMEARHVGQHR"
     gene            1287..1595
                     /locus_tag="ACHKTW_005241"
     CDS             1287..1595
                     /locus_tag="ACHKTW_005241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2730 family protein"
                     /protein_id="HFO6466081.1"
                     /translation="MWVSIVKDYVVPILSATATAGGIFMALMRKTFVPREAFEKLSDR
                     VEKVETRLSSLPTEAEVNRLNVEIVTLRGELKTTNATLRSVSYQNELLLEQAVRKKTQ
                     "
     gene            1592..>1818
                     /locus_tag="ACHKTW_005242"
     CDS             1592..>1818
                     /locus_tag="ACHKTW_005242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HFO6466082.1"
                     /translation="MSDFITEDQRLVILRSLADYNGELGESVLQDCLDDYGHRVSRDT
                     VHTHIAWLAEQGLVRKRVLINGYFIAELTGRG"
BASE COUNT          382 a          487 c          564 g          385 t
ORIGIN      
        1 gggaagggaa agaaaactcc acgtatatcg atcccaccgg tacaccaacc atctgttacg
       61 gccataccgg cccggatgtg aaaccgggta tgaccctgac ggatgaagaa tgcctggaac
      121 tactggaaaa ggacatgaaa tgggcctttg cggccattga tcggcgtgtt caggtgccgc
      181 ttacccgtgg tcagacggtg gcgctggctt cgtggatctt ctgggctggt gaaacgaact
      241 tccgcaactc cacgcttctg cgcctgatca atgccgggca gatgcccgcg tcctgtaagc
      301 agtacatccg ctggatttat tcgaaggggg tgaaactccc cggtcttgag gcccgccgtt
      361 cggcggatga atggttatgt cgctacgatt tgccgaaagt ctgaaccgct tctggcgacc
      421 gctcatgatt gcgctgctgt gtgccgttgt gctgctgcgg ggtgtgctgt ggctgcggtg
      481 aataatccct taccggccct gattgccgga ggctgtatgg cggcgctggg ggtgatgagc
      541 gtgctggctg ccgtggtatg gggaatgcac cagaagacgc agcgccttga ggacaataat
      601 catgtgcttg tgcgcgaacg ggacgaagcc cgtctggtgc tggcaaacca gcaacgcacc
      661 ctgcaactca tttcacaaat cagtgaggcg gccacgaatg aaaaacaaca gaacattcag
      721 cacagcgagg ggcagcagag cgttgtccgc cggtcgctgg cagcagtgcc tgcggcctct
      781 gcccctgttc ctgatgatgt ggctgatcgg gtgcgccggg ccgtctgtga aatacgtgcc
      841 tgtgaagccg gtgccgatcc ccgctgaatg gctggctgac tgcctggtgc ctcctgcgcc
      901 ggagccgttc acgtttgggg catcggtcac ttacaacctg caactgctgg cggtgatcaa
      961 gaactgcaac gtggacaagg ccagtattcg tcgtctggag gcgcggcgac aacatgaatt
     1021 tactgatatg gccggaacgc ctgctgttcc ggcaggaaag acgaagtaag gaaaagctat
     1081 ggatgattca gatcgtgctc aggctgtgat ggagcgggga tcagaacgcg ccctttgtaa
     1141 ccggctgaca cgtaagtgcc gggcaatgga tgctccgggc agacgtgtct gcgcagactg
     1201 tggcggagag atccccgccg cacgtcttgt tgctgtgccg gatgccatcc gttgtgtgaa
     1261 ctgccagaac atcatggagg cccgtcatgt gggtcagcat cgttaaagat tatgttgtgc
     1321 cgatcctttc ggcgacggca accgctggcg ggatattcat ggcgctgatg cgcaaaacgt
     1381 ttgtcccccg cgaggccttt gaaaaactct ccgaccgcgt tgagaaggtg gaaacccgcc
     1441 tgtcgtcact gccgacagaa gccgaggtca accgtctgaa tgtggaaatc gtgaccctgc
     1501 ggggtgaact gaaaaccacg aacgccacgc tccgctctgt ctcctatcag aacgaactgc
     1561 tgctggagca ggctgtaagg aaaaaaacgc aatgagtgat ttcattactg aagatcagcg
     1621 tctggtcatt ctgcggtcgc tggcagatta caacggtgaa ctgggtgaat ccgtgctcca
     1681 ggactgtctg gatgattacg gtcaccgcgt gtcccgcgac accgttcaca cccacattgc
     1741 ctggcttgcc gaacagggac tggtgcgcaa acgcgttctg attaacggtt atttcatcgc
     1801 agagctgacc ggccgggg
//
LOCUS       DAZFQD010000297         1733 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.303, whole genome shotgun sequence.
ACCESSION   DAZFQD010000297 DAZFQD010000000
VERSION     DAZFQD010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1733
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.303"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(94..1581)
                     /gene="yeeO"
                     /locus_tag="ACHKTW_005243"
     CDS             complement(94..1581)
                     /gene="yeeO"
                     /locus_tag="ACHKTW_005243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
                     /protein_id="HFO6466083.1"
                     /translation="MWFHFLQLRSALNISSALRQVVHGTRWHAKRKSYKVLFWREITP
                     LAVPIFMENACVLLMGVLSTFLVSWLGKDAMAGVGLADSFNMVIMAFFAAIDLGTTVV
                     VAFSLGKRDRRRARVATRQSLVIMTLFAVLLATLIHHFGEQIIDFVAGDATTEVKALA
                     LTYLELTVLSYPAAAITLIGSGALRGAGNTKIPLLINGSLNILNIIISGILIYGLFSW
                     PGLGFVGAGLGLTISRYIGAVAILWVLAIGFNPALRISLKSYFKPLNFSIIWEVMGIG
                     IPASVESVLFTSGRLLTQMFVAGMGTSVIAGNFIAFSIAALINLPGSALGSASTIITG
                     RRLGVGQIAQAEIQLRHVFWLSTLGLTAIAWLTAPFAGVMASFYTQDPQVKHVVVILI
                     WLNALFMPIWSASWVLPAGFKGARDARYAMWVSMLSMWGCRVVVGYVLGIMLGWGVVG
                     VWMGMFADWAVRAVLFYWRMVTGRWLWKYPRSEPQKCEKKPAVSE"
BASE COUNT          542 a          472 c          359 g          360 t
ORIGIN      
        1 gaacgaggcg aatattagcg atgcccaccc ataatgtcaa agcctgtttt ttaaatttga
       61 aatcgtttgc tgaaataatc tgcattttgt cgtttattcc gacacggctg gctttttttc
      121 acacttttgc ggttcggatc gagggtattt ccacagccaa cgtccagtaa ccattcgcca
      181 gtaaaacagc acggcccgca cagcccagtc ggcaaacatt cccatccaga caccaaccac
      241 accccagcca agcatgatgc ccagcacata accgacgaca acccggcaac cccacatgct
      301 caacatagaa acccacatgg cgtaacgggc atcgcgagca cctttaaatc cagcgggtag
      361 cacccatgag gcggaccaaa taggcataaa taaagcattt agccaaatca gaatcacaac
      421 gacatgttta acctgtggat cctgggtgta aaacgatgcc ataaccccgg caaagggagc
      481 cgttagccag gcgatggccg ttaatccaag agtggaaagc cagaacacat gccgcaactg
      541 aatctctgct tgcgctatct gccctacccc caaccttcgg cctgtaatga tcgtagaagc
      601 agagccgagc gcacttccgg gtaagttgat aagagccgca attgaaaacg cgataaaatt
      661 tccggcaata acactggtcc ccatcccggc aacgaacatt tgggttaata accgaccact
      721 ggtaaataac actgattcga cactcgctgg aataccaatc cccatgactt cccagataat
      781 gctaaaattc agcggtttaa aatagctctt taacgaaatc cttagcgcag gattaaaacc
      841 aatcgccagc acccacaaaa ttgcaactgc gccaatataa cgagaaatgg ttaaacccaa
      901 cccagccccg acaaatccca gtcccggcca ggagaaaagg ccgtaaatca atatgccgct
      961 aataataata ttaagaatat tcaggctacc gttaatcagt agcggtattt tcgtattccc
     1021 tgcaccacga agtgccccgc taccaatcaa agtgatggca gctgctggat aactgagtac
     1081 cgtcagctcc agataagtca acgccagtgc tttaacttct gtcgtggcat cacccgcgac
     1141 gaaatcaata atttgttcgc caaaatgatg aataagcgtt gccaacagta cggcaaacaa
     1201 cgtcatgatc accaatgact gccgcgtcgc caccctcgct cgtcgtcgat cccgcttacc
     1261 gagactaaat gccacaacga cagtagtacc aagatcgata gctgcaaaaa aagccataat
     1321 gaccatattg aagctgtccg ccaatcccac gccggccatc gcatcttttc ccagccagct
     1381 gaccagaaaa gtgctcagga cacccatcaa caggacacag gcattctcca tgaagatagg
     1441 aacagcaagc ggggttatct cgcgccagaa caacactttg tagctcttgc gtttagcgtg
     1501 ccagcgagtg ccgtgaacaa cctggcgtaa agcagaggag atattcaaag ccgaccttaa
     1561 ttgcagaaag tgaaaccaca tttcaaataa tgagggagaa tcagcaaagc tgcaaagatt
     1621 ttcgccaaca aattgtctgc aaatgcaaca aactgttgat agaaacggca aacagttggg
     1681 gaatttaaaa atcgggtttg acaaaagatt tttcgccgtt aagatgtgcc tca
//
LOCUS       DAZFQD010000298         1693 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.304, whole genome shotgun sequence.
ACCESSION   DAZFQD010000298 DAZFQD010000000
VERSION     DAZFQD010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1693
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.304"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1284..1499
                     /locus_tag="ACHKTW_005244"
                     /pseudo
     CDS             1284..1499
                     /locus_tag="ACHKTW_005244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309389.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          500 a          291 c          355 g          547 t
ORIGIN      
        1 atgccagtta acagctatcc gtatggcgtg ttcggttttg cgagtaaaaa atggttactg
       61 tagaagcgga tttccaccac atgtcaacca tgaatggtca gtggtgcgag acgactggga
      121 tgatttggtc gatgacttct tccggcgaat actgatagaa acattgaaat attgtaagag
      181 aaagcgttca aaattctgtg gcacgtgaaa aattctacag cgtcatcacc ttattcagtc
      241 gaccttcaaa ataaacgttt aacagagaca atcaccattt ggattgtccg ctgaggaggc
      301 gaactatttc ctagacagct ttatcatgtc tgcccacatt gttggcgctg acaatactaa
      361 ggacaaacga tggggaagta gctttttagc gttattttcg ggagtaaaaa acgtggtatc
      421 tttccgtatg tcagttgtat cttgctggta ttcgtagtcg tgatagagca tacaacgata
      481 tttatcttga cgttagtgaa gaataagttg ttttgtgatc tatgttaatc tgggattaca
      541 atccgggctt gatacatcgc atcacgactt ttattctctt cagaatagaa tatgttactc
      601 cccgttttac aggctcagga tttgaattac aaattatact tttaagtgaa caataaccag
      661 gggtatttct tatttcctga aacctggttt ggcccatttc gggatgagtt tgaaaaaact
      721 cttgacgcat acgatagtga tgaaataaac gaaacaaatt acgtcaacaa actcaggcgt
      781 cttgcctgac atgctcctga catcattgat attcacgctc atctggcgaa tgttcattta
      841 cagcgtcatc aggatgccgt tatactcatt gataaaatac taacatataa ccctgagaat
      901 aatcaggggc cacgctggtt actgggggcg gaattgttac gtgctggcaa gcatgacagg
      961 gctctgtacg tattgacagg acatgtcaat gaattttccc cctactggta tgagcttgct
     1021 cttttacatt ttatgaatga ggcatatatg aatgctgcaa cggcatttcg atatggattt
     1081 ttaacaagca tatattgttg aaatgctttg ttagaatctt catccattcc ctggggctgt
     1141 atagtatgat ttttcaggta tttctgaata ctgccaaata ttattgcaaa acattctcta
     1201 aattatgaat taaatatcca taagcattat tacctgggaa ctggacatat aatcactctt
     1261 ctgtgctaaa ggaacgttct gaaatgttta agtatgcaga gatgttgatg gcggaagaag
     1321 atgataaagt atgtgaactt atttttaaac aacaagataa tttgatttta aaaataaatg
     1381 atagatttcc cagaataatt ataaagaaat accgaacatt acaaggtaaa gtcgtatggc
     1441 tatggatatt gccatttgtt attgggtggt taatcgtgcg tgctttgggt gtaaacagtt
     1501 ggcaaggtaa aagtaaatat tctgattatt atttgttaag aagtgagatg cctgtgcgtc
     1561 tggtgtctat tatattccca acattattgg cgctattctt gctgtcgtag gcatcgccat
     1621 actatgaata ttgtgtttgt cgtgattcaa tttttattta taacctctac ccggagcaaa
     1681 ttcaggtagg tca
//
LOCUS       DAZFQD010000299         1645 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.306, whole genome shotgun sequence.
ACCESSION   DAZFQD010000299 DAZFQD010000000
VERSION     DAZFQD010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1645
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.306"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            65..280
                     /locus_tag="ACHKTW_005245"
     CDS             65..280
                     /locus_tag="ACHKTW_005245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFO6466084.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTYLTNLYFKIKEDRRKAARGE"
     gene            284..625
                     /locus_tag="ACHKTW_005246"
                     /pseudo
     CDS             284..625
                     /locus_tag="ACHKTW_005246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309558.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdfR family protein"
     gene            complement(867..1127)
                     /locus_tag="ACHKTW_005247"
     CDS             complement(867..1127)
                     /locus_tag="ACHKTW_005247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001306174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6466085.1"
                     /translation="MDYPNLPKQTFADLIALRQAVVALINVLPDEEKELVKAILNRTA
                     ADFSSFPLTDDLADLPELIAASAIKLTEEIYSPQKPSQNSCE"
     gene            1239..>1645
                     /locus_tag="ACHKTW_005248"
     CDS             1239..>1645
                     /locus_tag="ACHKTW_005248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO6466086.1"
                     /translation="MNAKIKYGLSAAVLALIAAGASAPEILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGAIMVDGKPVVPGMKLSKEKCDQVNAIERDKALAWVEKNIRVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRINAGDRRGA"
BASE COUNT          440 a          344 c          463 g          398 t
ORIGIN      
        1 gggtgtcggt gtgagctgat gccccacttg ttgttgtgag cttccggatt gcgggagacg
       61 gggtatgtac cagatggaaa aaatcacaac aggtgtgtca tacaccacgt cagcggtggg
      121 aacgggctac tggttcctgc agttgctgga cagggtttcc ccgtctcagt gggcggcaat
      181 aggcgtgctg gggagtctgc tgtttgggct gctgacatat ctgacgaacc tgtatttcaa
      241 aattaaagaa gaccggcgta aggcggcgcg gggagagtag acgatgaata aacaatacga
      301 actggttgtt aaagggataa ataattaccc ggataagatt actgttactg tggcactgga
      361 aattgccggg cagccgtcgt tgttgtcgcc atatgtggcg attagtcttg accgtactga
      421 aggtgccacg ctggaatttt acgaagctga ggcgaaaaaa caggcgaagc agtttttcat
      481 ggatattgct gccgctttat gtgaagggga tgagcagtcg ccggaaaagc gccccataat
      541 tttagaggcg caggatgtgt tgataaccta cagaggaaaa ctaccgggaa taattactgg
      601 ttctctgaag acgccaccga cggcattgcg gtcagaaaac gatgatattg aatcacgcat
      661 tgaaaaactg gagtgctata tcgctgaatt gaaaaaaagc accccaacaa aaaatgaggt
      721 gcttgcagca gatgaaatga aagaaactat tcttgatcgc gcggcgcatc taagctgcgc
      781 ttcacggttg aaagagcatc ttcagcggcc tgaagaaaaa cgccgcgatg agcagttcgc
      841 ggcgttttac gattattgca ttgaagttac tcgcaggaat tttgtgaagg cttttgagga
      901 gagtaaatct cttcagtaag cttaatggcg gacgctgcaa ttaattcagg aaggtccgca
      961 aggtcatctg tcagtgggaa tgatgaaaaa tcggcggcag ttctgttaag aattgcttta
     1021 actaattcct tttcctcatc cggcaacacg ttgattagag ctacgactgc ttgcctgagt
     1081 gcgattaaat cagcaaaagt ttgttttggt agatttggat aatccatagt cacctctgtg
     1141 tttatcagat tgacataccc tggccagtgc ccaccactgg cggggtgaag gcttaacata
     1201 tccagggatt cggaaccgat aaatcctgat aaatatccat gaacgcaaaa atcaaatacg
     1261 gcctgtcggc tgccgttctg gcgctgattg ccgcaggggc ttctgcgcct gaaattctcg
     1321 accagtttct ggatgaaaag gaaggtaacc acaccacggc ataccgtgat ggtgcaggta
     1381 tctggaccat ctgccgtggt gccatcatgg tggatggcaa acctgtcgtt ccgggcatga
     1441 agttgtcgaa ggaaaaatgc gaccaggtta acgccattga gcgtgataaa gcgctggcgt
     1501 gggtggagaa aaacatcaga gtgccgctga ccgaacccca gaaagcgggg atcgcgtcat
     1561 tctgtccgta taacattggt cccggtaagt gtttcccgtc gacgttttat aaacgaatta
     1621 atgcaggcga tcgcagggga gcgtg
//
LOCUS       DAZFQD010000300         1636 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.307, whole genome shotgun sequence.
ACCESSION   DAZFQD010000300 DAZFQD010000000
VERSION     DAZFQD010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1636
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.307"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>1636
                     /locus_tag="ACHKTW_005249"
     CDS             <1..>1636
                     /locus_tag="ACHKTW_005249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000212852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO6466087.1"
                     /translation="DEFSSSQKVLMDISQRTGTAFSDNAALFARSAASMREYGYSADD
                     VLKVTEAISTGLKISGASTAEAGSVITQFSQALAQGVLRGEEFNSVNESGDRIIRALA
                     AGMGVARKDLKAMADDGQLTADKVVPALISQLGILRDEYAAMPETVADGITKVENAFM
                     AWVGGANEASGATKTLSGVLNGVAGNIDTVATAAGVLVAVGVARYFGNMASGVGKATT
                     ELLAAKSGTVALAAAQADAAVQVQRKAAADALAAERAWQLAQAELAVARNTSAEALVT
                     QNAVAKRRAMIVANAALVQSNHAVAASGKALENATSVTGMLKSGAASLLSLVGGIPGL
                     LMLGAGAWYTVYQRQEQARESARQYASTIDEIRAKLPGMTLPELADNEGKTREALEEQ
                     NRLIAEQESKVRGLKTQIADYQRWLQENPQGGSGAEIITHGLAEATGRLAVEQSRLTQ
                     MQERARSVQETLASLEYNRVARIREEAAEQNRAYQALLQMNVQHTEFNRLLGLGNELL
                     QQRQGLASVPLRLPQATLDDKQQSALNNTERQLALSR"
BASE COUNT          378 a          395 c          552 g          311 t
ORIGIN      
        1 gatgaatttt cgtcatcaca gaaagtactg atggatatca gccagcggac aggcaccgca
       61 ttttcggata atgcggccct gtttgcccgt tcggctgcct cgatgcgtga atatggttac
      121 agtgctgatg atgtgctgaa ggtgacggag gccatttcga cagggctgaa aatttccggt
      181 gccagtacgg cagaggcggg ttcggtgatc acccagttca gccaggcgct ggcacagggt
      241 gtgttacgcg gtgaggaatt taattcggtc aatgaaagtg gggaccggat cattcgcgca
      301 ctggctgcag gcatgggcgt ggcccgtaaa gatctgaagg cgatggcgga cgatggtcaa
      361 ctgacggcgg ataaagtcgt tcccgcgtta atcagccagc tggggatatt gcgtgatgaa
      421 tatgcagcca tgccggaaac ggtggctgac gggatcacga aggtggaaaa cgcctttatg
      481 gcctgggtgg gtggcgcgaa tgaggccagc ggagcgacga aaacgctctc cggcgtgctg
      541 aacggtgtgg ccggaaatat tgataccgtg gcaacagctg cgggtgtgct ggttgctgtc
      601 ggggtcgccc ggtactttgg caatatggca tccggtgtgg gaaaggccac gacagaactg
      661 cttgctgcga agtctggcac tgttgccctg gcagcggcgc aggcagatgc tgcggttcag
      721 gtgcaacgta aggcggcggc tgacgctctg gcggcagaac gtgcctggca actggcacag
      781 gcagaactcg ccgtggccag aaataccagt gcggaggcgc tggtcacaca gaatgccgtc
      841 gcaaaacgtc gggccatgat tgtggccaat gctgcactgg tgcagtcgaa tcatgcagtg
      901 gcggcctcgg ggaaggcgct ggagaatgcc acgtcggtta cgggaatgct gaaaagtggc
      961 gctgcatccc tgctttctct ggtcggcggg atacccggtt tactgatgct tggggccggt
     1021 gcctggtaca cggtgtacca gcgtcaggaa caggccaggg agtccgcgcg ccagtatgca
     1081 tcgacgattg atgaaatccg ggcaaagctg ccaggaatga cccttccgga actggctgat
     1141 aatgagggta aaacccgcga agcactggaa gagcagaatc gtctgattgc tgagcaggaa
     1201 agtaaagtcc ggggactgaa aacacagatt gctgactatc agcgctggct gcaggaaaat
     1261 ccacagggtg gttcaggtgc ggagattata acccacggcc ttgctgaggc aaccggcagg
     1321 ctggcggttg aacagtcacg actgacacag atgcaggaaa gggcgaggtc tgtacaggaa
     1381 acgctggcgt cactggagta caaccgtgta gcgaggatac gtgaggaggc ggcagagcag
     1441 aacagggcat accaggcatt actgcagatg aatgttcagc atacggaatt caaccgcctg
     1501 ctggggctgg gtaatgaact gctgcaacag cgtcagggac tggcgagtgt accgctgcga
     1561 ctgccacagg ccactctgga tgataaacag cagagcgccc tgaataacac agagcgtcaa
     1621 cttgccctgt cccggc
//
LOCUS       DAZFQD010000301         1624 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.308, whole genome shotgun sequence.
ACCESSION   DAZFQD010000301 DAZFQD010000000
VERSION     DAZFQD010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1624
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.308"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..162
                     /gene="ybcN"
                     /locus_tag="ACHKTW_005250"
     CDS             <1..162
                     /gene="ybcN"
                     /locus_tag="ACHKTW_005250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA base-flipping protein YbcN"
                     /protein_id="HFO6466088.1"
                     /translation="ITTIQSLRHTSDLDTGEMYVFLCKVEAWAVNIGCHLTIPQSCEF
                     QLLRDKQEA"
     gene            162..332
                     /gene="ninE"
                     /locus_tag="ACHKTW_005251"
     CDS             162..332
                     /gene="ninE"
                     /locus_tag="ACHKTW_005251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HFO6466089.1"
                     /translation="MATPLIRVMNGHIYRVSNRRKRKPELKPSEIPTLLGYTASLVDK
                     KWLRLAARRNHG"
     gene            325..615
                     /gene="ybcO"
                     /locus_tag="ACHKTW_005252"
     CDS             325..615
                     /gene="ybcO"
                     /locus_tag="ACHKTW_005252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HFO6466090.1"
                     /translation="MADLRKAARGRECQVRIPGVCNGNSETSVLAHIRLAGLCGTGIK
                     PPDLIATIACSACHDEIDRRTHFVDAAYAKECALEGMARTQVIWLKEGVIKA"
     gene            612..974
                     /gene="rusA"
                     /locus_tag="ACHKTW_005253"
     CDS             612..974
                     /gene="rusA"
                     /locus_tag="ACHKTW_005253"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RusA"
                     /protein_id="HFO6466091.1"
                     /translation="MNTYSITLPWPPSNNRYYRHNRGRTHVSAEGQAYRDNVARIIKN
                     AMLDIGLAMPVKIRIECHMPDRRRRDLDNLQKAAFDALTKAGFWLDDAQVVDYRVVKM
                     PVTKGGRLELTITEMGNE"
     gene            971..1111
                     /gene="ylcG"
                     /locus_tag="ACHKTW_005254"
     CDS             971..1111
                     /gene="ylcG"
                     /locus_tag="ACHKTW_005254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcG family protein"
                     /protein_id="HFO6466092.1"
                     /translation="MMFEFYMAELLRHRWGHLRLYRFPGSVLTDYRILKNYAKTLTGA
                     GV"
     gene            1197..1580
                     /gene="quuD"
                     /locus_tag="ACHKTW_005255"
     CDS             1197..1580
                     /gene="quuD"
                     /locus_tag="ACHKTW_005255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein QuuD"
                     /protein_id="HFO6466093.1"
                     /translation="MRDIQMVLERWGAWAANNHEDVTWSSIAAGFKGLITSKVKSRPQ
                     CCDDDAMIICGCMARLKKNNSDLHDLLVDYYVVGMTFMSLAGKHCCSDGYIGKRLQKA
                     EGIIEGMLMALDIRLEMDIVVNNSN"
BASE COUNT          426 a          348 c          429 g          421 t
ORIGIN      
        1 atcaccacta tccagtcgtt acgccatacc tccgatcttg ataccggaga gatgtatgtc
       61 ttcctgtgta aggttgaagc ctgggcggtg aatattggct gccacctgac tattccgcag
      121 agctgcgagt tccagctgct ccgcgacaag caggaggcgt aatggctaca ccgcttattc
      181 gtgtcatgaa cggacacatc tacagagtat caaatcgtcg taagcgtaag cctgagctga
      241 agccatccga aataccaaca ctgctcggat ataccgctag cctggttgat aaaaaatggt
      301 tgcgactggc agcaaggagg aatcatggct gatttgagaa aagcagcgcg tggtcgggaa
      361 tgccaggtaa gaatccctgg cgtatgtaat ggcaattctg aaacgtctgt actggcacat
      421 atccggctgg ctggattgtg cggtaccggt atcaaaccgc cagacctgat tgccaccatt
      481 gcatgttctg cctgccacga cgaaatcgac cgccgcacac attttgtcga tgctgcatat
      541 gcaaaagaat gcgcgctgga aggtatggcg agaacacagg ttatctggct gaaagagggg
      601 gttattaagg cgtgaatacc tacagtatca cattaccctg gcctccgagc aataatcgct
      661 attaccgcca taatcgcggg cgcacgcacg tcagtgcaga ggggcaggca taccgcgata
      721 acgtcgcccg aatcattaaa aacgcaatgc tggatatcgg cctggctatg cctgtgaaaa
      781 tccgcattga gtgccacatg ccggatcgcc gtcgccgtga cctggataat ctgcaaaaag
      841 ccgcttttga cgcactcacc aaagcaggtt tctggctgga tgatgctcag gtcgttgatt
      901 accgcgttgt gaagatgcct gttaccaaag gtgggaggct ggaactgacc atcaccgaaa
      961 tggggaatga atgatgtttg agttttatat ggcagaactt cttcgccacc gctgggggca
     1021 tctgcgctta tatcgtttcc ccggttctgt tttgaccgat taccgaatac tgaagaatta
     1081 cgccaaaacc ctgacaggag caggagtatg aagtcagaga taacaatcaa ctaatactgt
     1141 tttgttgatt tttgcttgta attggcgttc tggtctgatt tttgtggagt aagttgatgc
     1201 gtgatattca gatggttctt gagcgttggg gagcgtgggc ggctaataat catgaagatg
     1261 tgacctggtc gtccattgcc gccggtttta agggattaat tacttcaaaa gtaaaatctc
     1321 gcccgcaatg ttgtgacgat gacgcgatga ttatttgcgg gtgcatggcc cgtctgaaaa
     1381 agaacaacag cgatttgcac gatttattag tagattatta tgtagtcggt atgacattca
     1441 tgtcactggc aggtaagcat tgctgctctg atggttatat cgggaaaagg ttacagaagg
     1501 ctgagggcat aattgaaggg atgttaatgg cattagatat ccggttagag atggatatcg
     1561 ttgttaataa ctctaattaa tatgccaatt gtttactaaa aattattaaa aatggggcgt
     1621 tgca
//
LOCUS       DAZFQD010000302         1565 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.309, whole genome shotgun sequence.
ACCESSION   DAZFQD010000302 DAZFQD010000000
VERSION     DAZFQD010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1565
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.309"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..178
                     /locus_tag="ACHKTW_005256"
     CDS             <1..178
                     /locus_tag="ACHKTW_005256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001620079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO6466094.1"
                     /translation="KNALVRIPVAGKVCAYALDPHEVWKGYNTSKNYAAFVTKTLVNK
                     DGDIQRRIMAMFSN"
     gene            1264..>1565
                     /locus_tag="ACHKTW_005257"
     CDS             1264..>1565
                     /locus_tag="ACHKTW_005257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001620079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO6466095.1"
                     /translation="MYIGTEEQRKKRLKELEKLNKESESDPKNNGPFTQVYQKGWERI
                     RELSKDKQGVVAIPLYSFLAEHIDPSCGAVVADQQFLADKLGVSRSTIKRWLNYLES"
BASE COUNT          407 a          383 c          412 g          363 t
ORIGIN      
        1 aaaaaacgcg ttagtcagaa ttcctgttgc tggtaaggtt tgtgcgtatg cactcgatcc
       61 acatgaagtc tggaagggat acaacacatc taaaaactac gcggcgtttg tcactaaaac
      121 actggtcaac aaagacggtg atattcagcg ccgaatcatg gccatgtttt caaattgagc
      181 cagcggcagg cacaggcaaa aaccccgttt ttggacaatc tgattcaaat gttaacattt
      241 gacatcgggt tgtctatcct gcattgctct tttgccgcct caaaatccct tgcgtgtttt
      301 tgctccccga tctccagaac gatcgagccg ccacggttcc ggcagcgcct tgagcgcaac
      361 cggaaaccgt ctggggagaa ccccagaccc ccatcgctgt aacaccgacg gtctacgacc
      421 acctaccaac cgagtggctg cctgtaccgt gcaaatgggc tttgcagggg gctggcgcgg
      481 ttcctggcga cctgaaagcc cctggctagg ggtatcggcg cgcctgacct cctgaaacgc
      541 tctgtaagcg tttttcgcgc cttccatgcc ctgacgggca tttaaacccc gtttcgtgcg
      601 tctgagagct tctgagcgcg tttgaagggc tatctgacga cttatccccg aaagaacaac
      661 agcggaatca ccgacaacat gcgagaggtg gcaccgctcg ccgcagcccc agcgaccgaa
      721 cgttagtgag cgagtgggcg aggaagcgga agagaaccgg aaaccacatt gagcacgtac
      781 gcactgatgc ggggtgtcgg ggcgaagccc tgaccagatg gcaaacggaa tatcgtcgcg
      841 ggtgacggta ttacatttgc acatcctgtc ccgatttctg aggcgtttta gcggtgaacg
      901 gacggcaaaa ccatgatgga gctgttgaac gttgagagcg tgaaatctgg caaaaatcac
      961 agctggtaag gcttgcagag gagtaagcca gtatacactc cgctatcgct acgtgactgg
     1021 gtcagggctt cgccccgaca ccccaaaaaa gtgttccgct ttgcgcaaca ctttcagcag
     1081 aaaagatatt taagggcgat tttgtaagtt attgattttt tagctacttg cattatgggt
     1141 tagctcacgc atgtgtggtc taaggttagc tcacgcatgt gtgagctagg tcgacctatc
     1201 atggtttacg tttgagctat gatagcttat atttgagcca tcattagacc gtggagattt
     1261 accatgtata tcgggactga agagcagcga aaaaagcgtc taaaagaact ggaaaaatta
     1321 aacaaagaat cggaatcaga tccgaaaaat aacggcccat tcacccaggt ttatcagaag
     1381 ggctgggaaa ggatccgaga actgtcgaag gacaagcagg gagttgtagc aataccgctt
     1441 tactcgtttt tagctgagca catagatccc tcatgcggtg ctgttgttgc cgatcagcag
     1501 tttttagcag ataaattggg tgttagccga agcacaatta agcgctggct caactactta
     1561 gaatc
//
LOCUS       DAZFQD010000303         1564 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.310, whole genome shotgun sequence.
ACCESSION   DAZFQD010000303 DAZFQD010000000
VERSION     DAZFQD010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1564
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.310"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(1..1564)
                     /locus_tag="ACHKTW_005258"
                     /pseudo
     CDS             complement(1..1564)
                     /locus_tag="ACHKTW_005258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309571.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
BASE COUNT          319 a          489 c          381 g          375 t
ORIGIN      
        1 ccgtccgccg gctgcggaat atttgcgacc gcatcattca ccgccttctg cagtacttcc
       61 ggatcgtagt cacgaccgtc acgcggaaca ggaatatggc ttaccgcctc cttcactatc
      121 tgttcaagca tcggacgcac atcatcgccc gtcacgcact tctgtaatac cacagacagg
      181 gaagccagtt tttcttcaaa cgtttgtgcc tgcgcggcta ttttctcctc aaatgtgcgc
      241 tgtaaatccg ccagcaccgc ggaaaattct tcacccagcg cacggataat ggacagttca
      301 cgttcattca ttttttcaga atccccctga acatcgcctt caccgcgtca tgctctgttt
      361 cagtgattgc cttattaccg tcagatgcag cgaccgcctg aggcacggaa gctgttttac
      421 cggacgacgc gaacggatcc tcacgggcat cacggcggga caacgcttcc agactgtagt
      481 tctgctgctg aagatacagt gcatcgcctc ctgccagggg cggcaggttc tcccgttttc
      541 gggcttcatt gggcgtgaga agcgtatttt tcaccgcatc ccccagcgtt ttcatgcgcc
      601 gctcactgtc cattctcagc agcgtggtga catcaaattc cgtactctcg ttttcccccg
      661 tttccagcgc ctcatccagt aacagctcaa tggactcaat cagtgtctgc aggcactggg
      721 aataatactg ctgctccagc gcctccacgt tgtcactgga aggcggttgt cccacgccaa
      781 tcttgtaggc cgggacacgg aacaccgaac agacaatttc agcggtcatc ttcagttgtt
      841 ccaccgtctg cgcatccacc ggtgaaaacg tcgtggggtt gtattttgcc ccgttgctca
      901 gaatggccgt tttccccgca ttttcgcctg tatacccgct gtcccagttg ctcttcagtt
      961 ttttcgcatt ttcttccgta atactgccgg ggatctcaat caccccggac ggcctgccgc
     1021 catttctgaa aaaagacgtc gaattttcct gaatatgatg cccctgcgtg gccgccagcc
     1081 cggcggcata caccggcggc aatcctataa gcggatgaaa aaaacagtta aaccggtcgt
     1141 ggatcacttc ccgggcaggc accgtcaccg cctcagtgat cccgcagttc cggtccggcg
     1201 tgatgcggta gaacacctcg ccgtcatccg ccaccagagg ttcaacccgg ttccagtcca
     1261 gaatacgcag ttctttgatc tgcccccggg cattacggat tttcagcacc accgtattgc
     1321 cgtgacgcag tttggcgttc agccacagtt caaaaaactg gatgcggttc tgctgggcgt
     1381 tgggacgacg acagaggcgg gcaatatccc cccggcgcgt ttccctgcgt atcccctgcg
     1441 catccgtctg cataagacgc agccgcattt tggcgatatc ctgggatatc agcgaaatac
     1501 atgcaaacac cgcatgaaag gagaggacgg cttcaggatc ggctttcacg ccctgctgcc
     1561 aggc
//
LOCUS       DAZFQD010000304         1549 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.311, whole genome shotgun sequence.
ACCESSION   DAZFQD010000304 DAZFQD010000000
VERSION     DAZFQD010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1549
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.311"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            351..476
                     /locus_tag="ACHKTW_005259"
     CDS             351..476
                     /locus_tag="ACHKTW_005259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001431667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFO6466096.1"
                     /translation="MWLPVLFRFLVDSLATLGHRLAASGITSRMLSVIPVLFFWG"
     gene            complement(921..1397)
                     /locus_tag="ACHKTW_005260"
     CDS             complement(921..1397)
                     /locus_tag="ACHKTW_005260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO6466097.1"
                     /translation="MFIDSEKRLKQLSDEAKKNTEDLEEAKKNSRFTQVSPKGWERVR
                     ELLKDSQGISALKLYSFLAEHIDPTCGAVVADQQFLAEKLGVSRSTIIRWLNYLESKN
                     ALVRIPVAGKVCAYALDPHEVWKGYNTTKNHAAFVTKTLVNKDGDIQRRIMAMFSN"
BASE COUNT          359 a          406 c          385 g          399 t
ORIGIN      
        1 aaaaaaacaa ccacttacaa atcggcgtaa aagatctatt ctaggcaagg gtgttgcgtg
       61 caacgcaacg ccctttgggg gtgtcggggc gaagccctga cccagtcacg tagcgatagc
      121 ggagtgtata ctggcttact cctatgcaag cctcgccagc cgtgattttt gccagatttc
      181 gtggttttca cgttcaacag ctccatcgtg tttttgccgt ccgttcaccg ttaaaacgcc
      241 tcagaaatcg ggacaggatg tgtaatcgta taaccgcgca tgcacgccga tacgattacc
      301 acttggtcag ggcttcaccc cgacaccccg catcagtgcg taagtgctca atgtggcttc
      361 cggttctctt ccgcttcctc gtcgattcgc tcgctacgct cggtcatcga cttgcggcga
      421 gcgggatcac ctctcgcatg ttgtcggtga tcccggtgtt gttcttttgg ggatgagtcg
      481 ccagatagcc cctcaaacgc gctcaaaagc tctcagacgc acgaaacggg ttttagatgc
      541 ccgccagggc atggaaggcg cgaaaaacgc ttacagaggc tctcaggagg tcaagcgcgc
      601 cgatacccct aaccaggggc tttcaggtcg ccaggaaccg cgccagcccc ctgcaaagcc
      661 catttgcacg gaacaggcag ccatgcggtt ggtaggtggt cgtagaccgt cggtgttgca
      721 gcgatggggg tctggggttc tccccagacg gtttccggta gcgctcaagg cgctgccgga
      781 accgtggcgg ctcggttttt tctggagaac ggggagcaaa aacacgcaaa ggattttgag
      841 gcggcaaaag agcaatgcag gatagacaat catggtcaga acgttaacac gtagcccctg
      901 attgtccgcc tgccgctagc tcaatttgaa aacatggcca tgattcggcg ctgaatatca
      961 ccgtctttgt tgaccagtgt tttagtgaca aacgctgcat ggtttttcgt agtgttgtat
     1021 cccttccaga cttcatgtgg atcgagggca tacgcacaaa ccttaccagc aacggggatt
     1081 ctaactaatg cattttttga ttctaagtaa ttgagccacc gaataattgt gcttctgcta
     1141 actccaagtt tttcagctag gaattgctga tccgcaacga cagcgccaca cgtaggatcg
     1201 atatgctccg ctaaaaatga gtacagcttc agtgctgata tgccttggct atccttcagc
     1261 agctctcgaa cacgttccca accttttggg gatacctgtg taaaccttga atttttcttt
     1321 gcttcttcga gatcctcggt gtttttcttt gcctcatctg aaagttgttt cagtcgtttt
     1381 tctgaatcaa taaacatgat tttttccctc tgaaaatccc atgcactaac gttggttcaa
     1441 atctaagcca tcatgacaca tctgtgcaac accttgttgc gtgttgtcgc atccaggatg
     1501 cgacaacgtt gtgtcatcca ggatgcgaca ggtaaataaa aaacacact
//
LOCUS       DAZFQD010000305         1544 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.312, whole genome shotgun sequence.
ACCESSION   DAZFQD010000305 DAZFQD010000000
VERSION     DAZFQD010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1544
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.312"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(1..558)
                     /locus_tag="ACHKTW_005261"
     CDS             complement(1..558)
                     /locus_tag="ACHKTW_005261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative phage tail protein"
                     /protein_id="HFO6466098.1"
                     /translation="MAVTLTPHQRALLQLLPDGLVWDKRPSSVLAALCLGLSHSTERV
                     SWTGNQMLAERFPDSSRLLLEDWERYLGLPECDMTGATIQERQRYAGNKYRMKPSLNR
                     EFYIRFAAEFGYEIDIQPSPESQWISIVTVKTAVGYRHMNVLDDILTPLRIYDASALE
                     CILNRYKPAWQTFLYLYENSHEETE"
     gene            complement(549..>1544)
                     /locus_tag="ACHKTW_005262"
     CDS             complement(549..>1544)
                     /locus_tag="ACHKTW_005262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313014.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HFO6466099.1"
                     /translation="VNETTLNAIAYAQAGLSAQEHEHLAWISRQIIPTEADEAELLKH
                     CAFWGVIRKPASRADGPVQLMLTTDAGITEGVLLQRSDGVVYRITGSATGKAGTLNVN
                     VEAESAGRAGNTPTGTRLSFITPQAGINQTATVTGTGLTGGADVETVPELLSRLVFRV
                     QNPPSGGTQYDFERWAREVPGVTRAWCKPEWPEAGSVGVTFVQDNNPDIFPGEGDVKR
                     VADYIRSHDDPATGQPVGQPLGPTISVFKLTNKPVVFEIRIVPKTPENQAAVKQALTD
                     LLYNESRPGGLVLPSSFWRAVAGVKGLEDFEVRSPLKSVMAGDTELLTVGEITWL"
BASE COUNT          343 a          466 c          376 g          359 t
ORIGIN      
        1 tcactccgtc tcctcatgac tgttttcgta cagatataaa aacgtctgcc aggctggctt
       61 ataacggttt aaaatgcact ccagcgcact tgcatcataa attcgcagtg gcgtaagaat
      121 atcgtccagt acattcatgt gacggtatcc aaccgctgtc ttcactgtca caatactgat
      181 ccattgtgat tccggtgatg gctggatatc tatttcataa ccaaactctg ccgcaaaccg
      241 gatataaaat tcacggttaa gagagggttt catccggtat ttattcccgg cataacgctg
      301 acgctcctga atggttgcgc cggtcatatc acattccggt aaccccagat aacgctccca
      361 gtcttccagc agcagacggg atgaatcagg aaaacgttct gccagcatct ggttgccggt
      421 ccaggaaaca cgctccgtgg aatgactgag gcccaggcac aaagccgcaa gaacggatga
      481 cggccgctta tcccatacca gcccgtcagg cagcaactgc aacagggcgc gctgatgcgg
      541 ggtcagggtt acagccatgt gatttccccc acggtcagca actctgtatc tccggccatc
      601 acggacttca gcgggctgcg aacttcaaaa tcctccagtc ctttcacccc tgcaacagcc
      661 cgccagaatg atgaaggcaa tacaagtcca cccggccgcg attcgttata aagcaggtcc
      721 gttaatgcct gttttacggc agcctgattt tccggcgttt tgggtacaat ccttatctca
      781 aacaccaccg gcttattggt cagcttaaac acgctgattg tcggcccaag tggctgacca
      841 acgggctggc ccgtcgccgg atcatcatga ctgcggatat aatccgccac ccgcttcaca
      901 tcaccttccc cggggaaaat gtcagggtta ttatcctgaa caaaagtcac accaacacta
      961 ccagcctcag gccattcagg cttacaccat gcccgcgtca cgccaggtac ttcccgtgcc
     1021 cagcgttcaa aatcatactg tgttccccct gatggcgggt tctgtacccg gaataccagc
     1081 ctggacagca actccggcac cgtttccaca tccgcaccac cggtgagtcc cgtgccggtg
     1141 accgtggctg tctggttgat gcccgcctgt ggtgtgataa aggacaggcg ggttccggtc
     1201 ggggtatttc cggcgcgccc cgcactttcc gcctccacat taacattcag tgttccggct
     1261 tttccggtcg cagagccagt gatgcggtac acaacaccat cgcttcgctg aaggagtacg
     1321 ccttccgtga tccctgcatc cgtggtcagc atcagttgta ccggtccgtc agcgcgggaa
     1381 gccggtttac ggatgacacc ccagaatgcg cagtgtttca ggagttcggc ttcatcagct
     1441 tctgtcggaa ttatctgccg ggagatccag gccagatgtt catgctcctg tgctgacagc
     1501 cccgcctgtg catacgcaat ggcattcagg gtggtttcat tcac
//
LOCUS       DAZFQD010000306         1536 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.313, whole genome shotgun sequence.
ACCESSION   DAZFQD010000306 DAZFQD010000000
VERSION     DAZFQD010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1536
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.313"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..73
                     /locus_tag="ACHKTW_005263"
     CDS             <1..73
                     /locus_tag="ACHKTW_005263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HFO6466100.1"
                     /translation="PGVFARADRRPPPPALYHLFLLP"
     gene            complement(52..243)
                     /locus_tag="ACHKTW_005264"
     CDS             complement(52..243)
                     /locus_tag="ACHKTW_005264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6466101.1"
                     /translation="MNISTETREILRNYRAVINARRREMGQKPLTTAQIVDEICDFVA
                     NQQAVFLGGHYILQGSRNR"
     gene            complement(243..662)
                     /locus_tag="ACHKTW_005265"
     CDS             complement(243..662)
                     /locus_tag="ACHKTW_005265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000271690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HFO6466102.1"
                     /translation="MYCTVKEIIREVLDTDVPDSECVFAVVLTRGDVRHIAQDWSLTD
                     DELETVMQRLDDAFEHGADVSIVHDVVRELMEEKRVNRLVTVPAVLLEKVMVMAGSEI
                     YRLHAVGSENGGDGDAFVREEREIMRLMRQALDGENG"
     gene            complement(709..1134)
                     /locus_tag="ACHKTW_005266"
     CDS             complement(709..1134)
                     /locus_tag="ACHKTW_005266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001198918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFO6466103.1"
                     /translation="MQYAKPVTLNVEECDRLSFLPYLFGQDFLYAEASVYALAKQMMP
                     EYEGGFWHFIRLPDGGGYMMPDGDRFHMVNGANWFDRTVSADAAGIILTSLVINRQLW
                     LYHDSGDAGLTHLYRMRDAQLWSHIEFHPECNAIYAALD"
BASE COUNT          348 a          469 c          406 g          313 t
ORIGIN      
        1 gccaggggtt ttcgcgcggg ctgacaggcg gcctccaccg cctgccctgt atcacctgtt
       61 tctgctgccc tgaaggatat agtgaccacc gaggaaaacc gcctgctgat tcgccacaaa
      121 atcgcagatt tcatcaacaa tctgcgcagt ggtgagcggt ttctgcccca tttcacgacg
      181 ccgcgcatta atcacggccc tgtaattacg cagaatttcg cgggtttccg tgctgatatt
      241 cattacccgt tctccccgtc cagcgcctga cgcatcaacc gcataatctc acgttcttcc
      301 ctgacaaatg cgtcaccgtc gcccccgttc tcgctcccta cagcatggag gcgataaatt
      361 tcactaccag ccatcaccat cactttttcc agcagcaccg cagggactgt tacaagacgg
      421 tttacgcgct tttcctccat cagttcacga acaacgtcgt gaacaatgct gacatcagca
      481 ccatgctcaa aggcatcgtc cagccgctgc atgacggttt ccagctcatc gtctgtcaga
      541 ctccagtcct gggctatgtg acgcacatcc ccacgggtca gcaccacggc aaaaacgcat
      601 tcactgtccg gcacgtctgt atccagcact tcgcggataa tttctttaac agtacagtac
      661 atgttctcgt ctcctttaca ggggcggcat tgccgccccg tccgttaatc agtccagtgc
      721 cgcgtaaatc gcgttgcatt cggggtgaaa ttcgatgtgg ctccacaact gcgcatcgcg
      781 catccggtaa aggtgtgtca gtcccgcatc cccgctgtcg tggtacagcc acaactggcg
      841 gttaatcaca agggaagtca ggatgatacc tgcggcatca gcactcacgg tacggtcaaa
      901 ccagtttgca ccgttcacca tgtgaaaacg gtcgccatcc ggcatcatgt aaccgccacc
      961 gtccggcagg cggatgaagt gccagaatcc gccttcatat tccggcatca tttgtttcgc
     1021 cagcgcgtat acagacgctt cggcatacag aaaatcctgg ccaaacaggt aaggcagaaa
     1081 agacagacgg tcgcactctt caacgttcag agtgacaggt ttcgcatatt gcatgatgtt
     1141 ttcctccgtt gttcacatat tccgcgacac cgggcggtgc cgtgcctcca taaaagccgg
     1201 gcgataaggt ggggatgcac agggcgcaag ggaagccgca gcctgacgca cacggctggc
     1261 gggagttgga gggtgagacc gcgaaatctg agcgggtgaa cgaccggaaa atcacggcag
     1321 acgggaagga gggaccgggt gaccgcagcg gcgaagcgta cccttgcatc cctgccagcc
     1381 cggctaagat ggaggcacgg cacagcccgc acacgacacg gcggccacat aactgaaaaa
     1441 ccacaaggcc atgccctgca tggccgggta aagcggagcc ggcggcgacg tcctttgtgc
     1501 tctgtccggg gaccggcagg aaaaaggcgg ggattt
//
LOCUS       DAZFQD010000307         1521 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.314, whole genome shotgun sequence.
ACCESSION   DAZFQD010000307 DAZFQD010000000
VERSION     DAZFQD010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1521
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.314"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            2..1042
                     /locus_tag="ACHKTW_005267"
     CDS             2..1042
                     /locus_tag="ACHKTW_005267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiG family protein"
                     /protein_id="HFO6466104.1"
                     /translation="MKRNLLSSAIIVAIMALGLTGCDDKKAETETPPPANSQPAAPAP
                     EAPVAKAEAKPETPAQPVVDEQAVFDEKMDVYIKCYNKLQIPVQSSLARYADWLKDFK
                     QGPTGEERTVYGIYGISESNLAECEKGVKSAVALTPALQPIDGVAVSYIDAAVALGNT
                     INEMDKYYTQENYKDDAFAKGKTLHQTFLKNLEAFEPVAESYHAAIQEINDKRQLAEL
                     KNIEEREGKTFHYYSLAVMISAKQINNLISQEKFDVDAAMKKVSELETLVAQAKEADK
                     GGMNFSFINSAGQYQLEAKKYVRRIRDKVPYSDWDKEQLQDANSSWMVEDSFPRALRE
                     YNEMVDDYNSLR"
     gene            complement(1092..>1521)
                     /locus_tag="ACHKTW_005268"
     CDS             complement(1092..>1521)
                     /locus_tag="ACHKTW_005268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFO6466105.1"
                     /translation="PERFFSAPGSFHYSNVRGESWQHITRQICAQLVAQHHITADEAQ
                     RIIAREGEGENLIVNRLAIPHCWSEQERRFRGFFITLAQPVEVNNEVINHVLIACAAA
                     DARHELKIFSYLASILCQHPAEIIAGLTGYEAFMELLHKG"
BASE COUNT          452 a          334 c          375 g          360 t
ORIGIN      
        1 aatgaaaagg aatttattat cttccgctat tatagtcgcc atcatggccc tcggtctgac
       61 gggttgtgat gataaaaaag ccgaaacaga aacgcccccg cctgccaata gtcaacctgc
      121 cgcaccagct cctgaagctc ccgttgcaaa agcagaagct aaacctgaaa cacctgcgca
      181 accggtggtc gatgaacaag cggttttcga cgaaaaaatg gatgtctata tcaagtgcta
      241 taacaagtta cagatccctg tacagagcag tctggcgcgt tatgctgact ggctgaaaga
      301 ttttaaacag gggcctaccg gtgaagagcg tactgtttat ggcatctacg gcattagtga
      361 atccaacctc gctgagtgtg aaaaaggcgt aaaaagtgct gtggcgttaa cgcctgcgct
      421 gcaaccaatt gatggcgtgg cggtgagtta tatcgatgct gccgtggcgt tgggtaatac
      481 cattaacgaa atggataaat attacaccca ggaaaattat aaagacgatg cgtttgcgaa
      541 agggaaaacg ctgcaccaga cattcttaaa aaatctggaa gcctttgaac ctgtagcaga
      601 atcttatcat gcggcgattc aggaaattaa cgataagcgc cagcttgcgg aactgaaaaa
      661 tattgaagaa agagaaggga aaacattcca ctactactct ctggcagtca tgatttcagc
      721 gaaacaaatt aataatctga tatcgcaaga gaagtttgat gttgatgcgg caatgaagaa
      781 agtgtctgaa ctggaaacgc tagtggcgca ggccaaagaa gcggataaag gcggcatgaa
      841 tttctcgttt attaattcgg caggccagta tcaactcgag gctaaaaaat acgttcgccg
      901 catcagagat aaagtcccgt actctgactg ggataaagag caacttcagg acgcaaactc
      961 aagctggatg gtcgaagact ctttcccgag agcattacgc gagtacaacg aaatggttga
     1021 tgactataat agcctgcgtt aaacgttttt gatgataaat gcagtgtcgg atgcggcgca
     1081 tccgacacag ttcacccctt gtgaagtaac tccataaatg cctcatatcc tgttaacccg
     1141 gcaataatct ccgccggatg ctgacacaat atgcttgcca gatagctaaa tatttttagc
     1201 tcatgacgcg catcggcggc ggcgcaggcg atcaagacat ggttaatgac ttcgttattc
     1261 acctcaactg gttgggcgag ggtaataaaa aatccacgaa aacgtcgctc ctgttcgctc
     1321 cagcaatgtg ggatggcgag gcgattaaca atcaggtttt caccttcgcc ttcgcgggcg
     1381 atgatgcgtt gtgcttcatc ggcggtaata tggtgttgtg cgactaattg cgcacaaatt
     1441 tgccgggtaa tatgttgcca gctttcaccg cgtacattcg aataatgaaa acttccgggg
     1501 gcagagaaaa aacgctccgg t
//
LOCUS       DAZFQD010000308         1519 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.315, whole genome shotgun sequence.
ACCESSION   DAZFQD010000308 DAZFQD010000000
VERSION     DAZFQD010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1519
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.315"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1440
                     /locus_tag="ACHKTW_005269"
     CDS             <1..1440
                     /locus_tag="ACHKTW_005269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HFO6466106.1"
                     /translation="LSSSLAGLLVMSGGVSAQEEKYTVPYATGNNKWGNTYEVVKTGD
                     NGNFKYEVKEKKNGRKTLFTFDSNGDVIINGSGITYTIHDGALNDFARAAEKKKNGQS
                     QSHRMTDSVVRDVYNKVYSLQRTEITGFSVEDGENGKVSLGSDAKASREFSVAVGNGA
                     KATGKASTSVGSWSSAFGTASVAVGGPATQAYGDRSTSVGVGASVRPNATEGTAVGFK
                     ASVDENAADGVAIGASSTVAHRNSVALGSNSRTTREDEVYIGYEAEPGKAYKTRVLGG
                     LSDGTRPSDAATVRQVDRVKDSVEQLAQDTNTRLVVEAKKSREYTDSRTTVGVNSDGT
                     LTSADGATKTIAVNDGLVALSGRTDRIDAAVGSVDRRVTKNTQAIQSNTRQLQEHNAR
                     LNSQQRQIRENHEEMKRAAAQSAALAGLFQPYSVGKFNATAALGGYSDKQAIAVGVGY
                     RFNEQTAAKAGIAASDGDVSYNMGVNFEF"
BASE COUNT          414 a          311 c          436 g          358 t
ORIGIN      
        1 ctgtcatctt ctctggccgg gcttctggtg atgagtggtg gggtgagtgc gcaggaagaa
       61 aaatacacag tgccgtatgc cacaggtaac aataaatggg ggaataccta tgaagttgta
      121 aagacaggcg ataatggtaa cttcaaatac gaagtaaaag agaaaaaaaa cggccggaag
      181 acacttttca catttgattc aaatggggat gtaataatca atggcagtgg cattacgtac
      241 acaattcatg acggtgcgct gaacgatttt gctcgagcag ctgagaaaaa aaagaatgga
      301 cagtctcagt cgcacagaat gactgatagc gttgttcgtg atgtatataa caaagtatac
      361 agtctgcaga gaacagaaat tacaggtttc agcgtcgaag atggggagaa tgggaaggtt
      421 tctctgggta gtgatgcgaa agcctctcgt gagttctcag tagctgttgg taacggtgct
      481 aaggctacag gaaaagcgtc aacatctgtt ggctcatggt catcggcatt tggaactgca
      541 agcgtggcgg ttggtggccc tgcaacccag gcatatggtg atcgttcaac atctgttggt
      601 gttggggcat ctgttcgtcc aaatgccaca gaaggaacag ctgttggttt taaggcaagt
      661 gttgatgaga atgcagctga tggagttgca ataggtgcca gctcgacagt tgcccacaga
      721 aacagtgtgg ctcttggctc aaattcacgt acaacccgtg aagatgaagt ttatataggt
      781 tacgaagctg agccgggtaa ggcttataaa acccgggtgc ttggtggtct gagtgacggt
      841 actcgtccct cagatgctgc aaccgttcgt caggttgacc gtgtaaaaga cagtgttgag
      901 caactggctc aggatacgaa tacccgcctt gtggttgaag cgaaaaagtc tcgtgaatat
      961 acagactcca gaacgacagt tggtgttaac tccgacggaa ccctgaccag cgctgatggc
     1021 gctacaaaaa cgattgctgt taatgacggt ctcgttgctc tgtccggcag aacagaccgt
     1081 attgatgctg cagttggtag cgttgaccgc cgggttacta aaaatacaca ggctattcag
     1141 tccaatactc gccagttgca ggagcataat gcacgcctga acagccagca gcgccagatt
     1201 cgtgaaaacc acgaagagat gaagcgtgca gcagcacaga gtgcagcgct ggcgggtctt
     1261 ttccagccgt acagtgtggg gaaattcaac gccacggcag ccctgggtgg ttacagcgat
     1321 aaacaggcga ttgctgtagg tgttggttac cgtttcaacg agcagaccgc agcgaaagcg
     1381 ggtattgcag cgagtgatgg cgatgtgtcc tacaacatgg gcgttaactt cgagttttaa
     1441 ttgctgacag tgacatgaag catgacggca ggcgtggagt tttgcctgcc gttttttctc
     1501 atgtgagcat gaaacaggt
//
LOCUS       DAZFQD010000309         1512 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.316, whole genome shotgun sequence.
ACCESSION   DAZFQD010000309 DAZFQD010000000
VERSION     DAZFQD010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1512
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.316"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..918
                     /locus_tag="ACHKTW_005270"
     CDS             <1..918
                     /locus_tag="ACHKTW_005270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312997.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF935 domain-containing protein"
                     /protein_id="HFO6466107.1"
                     /translation="EFLEVYGLPMKVGKYPSGATPEQKSALMRAVMDIGRRTGGIIPA
                     GMSLEFQAAANGQADPFETMISWGERSISKAILGGTLTTEAGDKGARSLGEVHNEVRR
                     EIRDSDLRQLAATLNRDLVYPLYALNTAHAIDIRRLPRICFQTKEPGDITKITSAVMQ
                     LSTGMDISDPWIREQTGIPQPTPGEAIFRVRQSGNEPAQTDKEMPPEKQEKTEQTALA
                     ARLPEAKSSPRDELDDMGDAVPARRLQEAIDPVLEPVIDAIRTRGLAEALADLPALYR
                     EMDDSRLMTLLSDAMFAAEMKGMLDGTGD"
     gene            902..>1512
                     /locus_tag="ACHKTW_005271"
     CDS             902..>1512
                     /locus_tag="ACHKTW_005271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor head protein"
                     /protein_id="HFO6466108.1"
                     /translation="MAQGIDLGYAATLPSKEAVAYFRAKGVHISWNWFETDADVHARS
                     FTAAKVARLDVLTTLQVEVQRAVDEGISQKAFIRTLTPRLQKLGWWGKQIVVDSAGNA
                     EEVQLGSPRRLALIYNVNTRVAYNAGRYTQMMNNTDTHPFWQYVAVMDSRTRPAHSAL
                     NGLVFRYDDPFWNTHYPPNGWNCRCRVRPLSQARLDAMGLSVSS"
BASE COUNT          340 a          414 c          476 g          282 t
ORIGIN      
        1 gaatttctgg aggtgtacgg cctgccgatg aaggtcggga aatacccgtc cggggcaaca
       61 ccggagcaga aaagtgccct gatgcgggcg gtgatggata tcgggcgacg tacaggcggg
      121 atcatcccgg ccgggatgtc gctggagttt caggcggcag cgaacggtca ggccgatccg
      181 tttgaaacca tgatttcgtg gggggagcgt tccatctcca aagcaatact cggcggcacg
      241 ctgaccacgg aagccggaga caaaggcgcg cgctcgctgg gtgaagtgca taacgaggtg
      301 cgccgggaaa tcagggattc tgatttacgc cagctggccg ccacgctgaa ccgcgatctg
      361 gtgtatccgc tgtatgccct gaacaccgcc cacgccattg atatccgtcg cctgccgcgt
      421 atctgtttcc agacaaaaga gccgggggat atcaccaaaa tcaccagtgc ggtgatgcag
      481 ctcagtacgg ggatggatat ttctgatccc tggataaggg agcagaccgg tattccgcag
      541 cccacgcccg gtgaagccat cttccgtgtc cgtcagagtg gcaatgaacc tgctcagaca
      601 gacaaagaga tgccaccgga aaaacaggag aagacagagc agacagcgct ggctgccaga
      661 ctgccggaag cgaaaagcag cccccgtgat gaactggacg acatggggga tgcggtgcct
      721 gcccgccggt tacaggaggc catcgacccg gtactggagc cggtcattga tgccatcaga
      781 acgcgggggc tggcggaggc actggcagac ctgcccgccc tttaccgtga aatggatgat
      841 tcccgcctga tgacgctgct cagtgatgcc atgtttgctg cggaaatgaa ggggatgctg
      901 gatggcacag gggattgatt taggttatgc cgccaccctt ccctcaaaag aggcggtggc
      961 atacttccgc gccaaagggg tgcatatcag ctggaactgg ttcgaaacag acgcggatgt
     1021 tcatgcccgt tcattcacgg cggcaaaagt ggcacgcctg gacgtactga ccacactaca
     1081 ggtggaagtg cagcgtgctg ttgatgaggg gatttcacag aaagcattta tccgcacgct
     1141 gacaccccgc ctgcaaaagc tgggatggtg gggaaagcag attgtggtgg acagcgcagg
     1201 gaacgcggaa gaagtgcaac tgggcagtcc ccgtcgtctg gcgctgattt acaacgtgaa
     1261 cacccgcgtg gcttacaatg ccgggcgtta cacgcagatg atgaacaaca cggacacgca
     1321 tccgttctgg cagtatgtgg cggtgatgga cagccgcacc cgcccggcgc attccgccct
     1381 taacgggctg gtattccgct atgacgatcc gttctggaat acacactacc cgcccaatgg
     1441 ctggaactgt cgctgccgtg tccggccatt gtctcaggcc cgtctggatg caatgggact
     1501 ctctgtttca tc
//
LOCUS       DAZFQD010000310         1512 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.317, whole genome shotgun sequence.
ACCESSION   DAZFQD010000310 DAZFQD010000000
VERSION     DAZFQD010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1512
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.317"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..918
                     /locus_tag="ACHKTW_005272"
     CDS             <1..918
                     /locus_tag="ACHKTW_005272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312997.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF935 domain-containing protein"
                     /protein_id="HFO6466109.1"
                     /translation="EFLEVYGLPMKVGKYPSGATPEQKSALMRAVMDIGRRTGGIIPA
                     GMSLEFQAAANGQADPFETMISWGERSISKAILGGTLTTEAGDKGARSLGEVHNEVRR
                     EIRDSDLRQLAATLNRDLVYPLYALNTAHAIDIRRLPRICFQTKEPGDITKITSAVMQ
                     LSMGMDIPDPWVREQTGIPQPAPGEAIFRVRQSGNEPAQTDKEMPPEKQEKTEQTALA
                     ARLPEAKSSPRDELDDMGDAVPARRLQEAIDPVLEPVIDAIRTRGLADALADLPALYR
                     EMDDSRLMTLLSDAMFAAEMKGMLDGSGD"
     gene            902..>1512
                     /locus_tag="ACHKTW_005273"
     CDS             902..>1512
                     /locus_tag="ACHKTW_005273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor head protein"
                     /protein_id="HFO6466110.1"
                     /translation="MAQGIDLGYAATLPSKEAVAYFRAKGAHISWNWFETNADVHARS
                     FTAAKVARLDVLTTLQAEVQRAVDEGISQKAFIRTLTPRLQKLGWWGKQIVVDSAGNA
                     EEVQLGSPRRLALIYNVNTRVAYNAGRYTQMMNNTETHPFWQYVAVMDSRTRPAHSAL
                     NGLVFRYDDPFWNTHYPPNGWNCRCRVRPLSQARLDAMGLSVSS"
BASE COUNT          338 a          417 c          471 g          286 t
ORIGIN      
        1 gaatttctgg aggtgtacgg cctgccgatg aaggtcggta aatacccgtc cggggcaaca
       61 ccggagcaga aaagcgccct gatgcgggcg gtgatggata tcgggcgacg tacaggcggg
      121 atcatcccgg ccgggatgtc gctggagttt caggcagccg cgaacggtca ggccgatccg
      181 tttgaaacca tgatttcgtg gggggagcgt tccatctcca aagctatcct cggcggcacg
      241 ctgaccacgg aagccggaga caagggcgcg cgctcgctgg gtgaagtgca taacgaggtg
      301 cgccgggaaa tcagggattc tgatttacgt cagctggccg ccacgctgaa ccgcgatctg
      361 gtgtatccgc tgtatgccct gaacaccgcc cacgccattg atatccgtcg cctgccgcgt
      421 atctgtttcc agacaaaaga gccgggggat atcaccaaaa tcaccagtgc ggtgatgcag
      481 ctcagtatgg ggatggatat tcctgatccc tgggtacggg agcagaccgg tattccacag
      541 cctgcccccg gtgaagccat cttccgtgtc cgtcagagtg gcaatgaacc tgctcagaca
      601 gacaaagaga tgccaccgga aaaacaggag aagacagagc agacagcgct ggctgccaga
      661 ctgccggaag cgaaaagcag cccccgagat gaactggacg acatggggga tgcggtgcct
      721 gcccgccggt tacaggaggc catcgacccg gtactggagc cggtcattga tgccatcaga
      781 acgcgggggc tggcggatgc gctggcagac ctgcctgccc tttatcgtga aatggatgat
      841 tcccgcctga tgacgctgct cagtgatgcc atgttcgctg cggaaatgaa ggggatgctg
      901 gatggctcag gggattgatt taggttatgc cgctaccctt ccctcaaaag aggcggtggc
      961 atacttccgc gccaaagggg cgcatatcag ctggaactgg ttcgaaacaa acgcagatgt
     1021 tcatgcccgt tcattcacgg cggcaaaagt ggcacgcctg gacgtactga ccacactaca
     1081 ggcggaagtg cagcgtgctg ttgatgaggg gatttcacag aaagcattta tccgcacact
     1141 gacaccccgc ctgcaaaagc tgggatggtg gggaaagcag attgtggtgg acagcgccgg
     1201 taacgcagaa gaagtacaac tgggcagtcc ccgccgactg gcgctgattt acaacgtgaa
     1261 cacccgcgtg gcttacaatg ccgggcgtta cacgcagatg atgaacaaca cggagacgca
     1321 tccgttctgg cagtatgtgg ctgtgatgga cagccgcacc cgtccggcgc attccgccct
     1381 taacgggctg gtattccgct atgacgatcc gttctggaat acacactacc cgcccaatgg
     1441 ctggaactgc cgctgccgtg tgcggccatt gtctcaggcc cgtctggatg cgatgggact
     1501 ctctgtttca tc
//
LOCUS       DAZFQD010000311         1510 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.318, whole genome shotgun sequence.
ACCESSION   DAZFQD010000311 DAZFQD010000000
VERSION     DAZFQD010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1510
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.318"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..748
                     /locus_tag="ACHKTW_005274"
     CDS             <1..748
                     /locus_tag="ACHKTW_005274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFO6466111.1"
                     /translation="TPLISVITATFNSQRFILDTYKSLQAQSYKNWEWVVTDDASEDK
                     TVSILKELQSNDDRIKIFINADNKGAAWSRNNSMKESKGQYFAFIDSDDLWHPEKLSR
                     QIAFMGDNIDFSFTSYELVDVENKKLNKKIDITPILPLTYHQMLRKKATLGCSTVILR
                     VSAFPDVRMPDLRTGQDYATWLKILKRGTRAYLLPEVLTSYRIVNGSISRNKFKKALR
                     QWEIYRKYENLSFLYSCYCFSLHAFRAVVR"
     gene            753..1157
                     /locus_tag="ACHKTW_005275"
     CDS             753..1157
                     /locus_tag="ACHKTW_005275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FdtA/QdtA family cupin domain-containing
                     protein"
                     /protein_id="HFO6466112.1"
                     /translation="MNINLIELQQHGDERGALVSLECNKNIPFDIRRVYYIYNTNKNV
                     VRGYHAHRKLKQMAICVKGYCTFTLDDGFERISIKLDNPAQGLLIESCLWREMSDFSE
                     DCVLLVLADADYDELDYIREYETFKKIVRGIT"
     gene            1154..>1510
                     /locus_tag="ACHKTW_005276"
     CDS             1154..>1510
                     /locus_tag="ACHKTW_005276"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000605304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO6466113.1"
                     /translation="MIMHKGKTIYLSSVTVDVESARKIVELRNGDVQKKFLNPTKNSI
                     EDQLEWLGNYLERNKNGDEFYFFIKRIDTDEIIGTVRIYDIKRDIDSFCWGSWILNEL
                     KTHTAALESYLLINKFA"
BASE COUNT          536 a          181 c          308 g          485 t
ORIGIN      
        1 tacaccatta atttcagtta ttacggctac atttaattct cagcgtttta ttctggatac
       61 ttacaaaagt ctccaggcgc agagctataa aaactgggaa tgggtcgtta ccgatgatgc
      121 ctcagaagat aaaacggtaa gtattttaaa agagttacag tcaaatgatg acaggataaa
      181 aatatttatt aatgctgaca acaaaggagc tgcgtggagt agaaataata gtatgaagga
      241 aagtaaaggt caatattttg ctttcattga tagtgatgat ctatggcatc cagagaagtt
      301 gtctaggcaa atagcattca tgggtgataa tattgatttt tcttttacca gttatgagtt
      361 ggtagatgtt gaaaataaaa aattaaataa aaagatagat attacaccta tattaccttt
      421 aacttatcat cagatgttaa ggaaaaaagc tactttaggt tgttcaactg ttatattgcg
      481 tgttagcgct tttcctgatg ttcgtatgcc ggacttaaga actggacaag attatgctac
      541 ttggttaaaa atacttaaaa gaggcacaag agcatacttg cttcctgagg tattaacatc
      601 ttatagaatt gtgaatggtt caatatcaag gaataaattt aaaaaagcat taaggcaatg
      661 ggaaatttat agaaaatatg aaaatttgag ctttttatat agctgctatt gtttttccct
      721 tcatgcgttc agagctgtag ttaggtaaga aaatgaatat aaatttaatt gaacttcaac
      781 agcatggtga tgagagaggg gcattagtat ctcttgagtg taacaaaaat atccccttcg
      841 atataagacg tgtttattat atatataata caaataaaaa tgttgtcaga ggttaccatg
      901 cacatagaaa gctgaaacaa atggcgattt gtgtgaaggg ctattgtact tttactttag
      961 atgatggatt tgaacgaata tctattaaat tagataatcc tgcgcaaggt ttgctaattg
     1021 agtcttgctt atggagagaa atgtctgatt tttcagagga ttgtgtgttg ttagtattag
     1081 cagatgcaga ttatgatgaa cttgactata ttagagaata tgaaacattt aaaaagattg
     1141 ttagggggat aacatgatta tgcataaagg aaaaacaata tatctatcca gtgtcacagt
     1201 agatgtcgaa agcgcacgga agatagtaga gctaaggaat ggcgatgtgc aaaaaaaatt
     1261 tttaaatccc actaagaatt ctatcgaaga tcaacttgaa tggttaggga attatttaga
     1321 aagaaataag aatggagatg aattttattt ttttattaaa cggattgata ctgatgagat
     1381 tataggaacg gttaggatat atgatattaa acgtgatata gattcttttt gctggggtag
     1441 ctggatcttg aatgagctta aaactcacac cgctgcatta gaaagttatt tactgataaa
     1501 taaatttgcg
//
LOCUS       DAZFQD010000312         1463 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.319, whole genome shotgun sequence.
ACCESSION   DAZFQD010000312 DAZFQD010000000
VERSION     DAZFQD010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1463
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.319"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            65..322
                     /locus_tag="ACHKTW_005277"
     CDS             65..322
                     /locus_tag="ACHKTW_005277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6466114.1"
                     /translation="MVTNPQIPLIGLYVSKVNPSNRIVVTNVHIVKDDDDEPGDLPFY
                     LVTFVNEGDEDDMSAPSWELDPDEWEQLVDEKLFMRVEQPS"
     gene            357..1358
                     /locus_tag="ACHKTW_005278"
     CDS             357..1358
                     /locus_tag="ACHKTW_005278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO6466115.1"
                     /translation="MSVRKLSTGKWLCECYPYGAAGKRIRKQFATKGEALSYERRLMN
                     GNLGGEFQDGSGPRLSELVARWFEMYGKTLSSGEERKAKLEAICSRLGDPFASQFDKN
                     MFAIYRERRLSGEWNPKGKKKLSEATVNREQSYLHAVFAELKRLGEWSGENPLTGIRK
                     FREEEKEQAFLYADEIERLLIACDESRNKDLGVVVRIGLATGARWSEAEGLKQSQILP
                     GRITFVKTKGKKNRTVPISPQLQAMLPKKRGALFSPCYEAFDAAIKRAKIELPDGQLT
                     HVLRHTFASHFMMRGGNILVLQKILGHSDIKMTMRYAHFAPGHLEAAVELNPFDNRG"
BASE COUNT          411 a          251 c          365 g          436 t
ORIGIN      
        1 ttgttatggt cttaaagaaa agtaagtctt tgttcatgag gaagtccaaa gaggtaaatg
       61 aattatggta actaatccgc aaattccatt aattggttta tatgtatcta aagttaatcc
      121 atctaatcgt atagttgtta ctaatgttca tattgtcaag gacgatgacg atgagcccgg
      181 agatcttcct ttctatttgg ttacttttgt aaatgagggg gatgaggatg atatgtcggc
      241 tccttcttgg gagcttgatc ccgatgaatg ggagcagtta gttgatgaaa agctctttat
      301 gcgggttgag caaccatctt aatcacgaaa attttccttg gtgtgcactt tttaatatgt
      361 ctgttcgtaa actatctact ggtaaatggc tctgtgaatg ctatccctac ggagcagcag
      421 gaaaacgcat tcgtaagcag tttgctacta aaggtgaggc gctctcttat gagcgccgtt
      481 taatgaacgg taatttaggg ggcgagtttc aggatggttc tggcccccgt ctttccgagt
      541 tggttgctcg ctggtttgaa atgtacggta aaacgttgtc ttccggtgaa gagcgcaagg
      601 ctaaacttga ggcgatttgt tctaggttgg gtgatccgtt tgcttctcaa tttgataaaa
      661 atatgtttgc catctacagg gagcggcggt tatcaggtga atggaatcca aaggggaaga
      721 aaaaacttag tgaagcaacc gttaatcgcg aacagtcata tttacatgct gtttttgctg
      781 aactgaagcg ccttggggag tggtctggtg aaaatccatt gacaggtatt cgcaagtttc
      841 gtgaggaaga aaaggaacag gcgtttctgt atgcagatga gattgaacgc cttctgattg
      901 catgtgatga gtcacggaat aaagatttgg gtgttgtcgt ccgtattggg cttgcgactg
      961 gtgccaggtg gagtgaagca gagggattaa agcaatctca aatactgcct ggacgaatca
     1021 catttgttaa aaccaaagga aagaagaacc gcactgtacc tatttcacct caattgcagg
     1081 ccatgcttcc taaaaaacgc ggggcgcttt tttcaccatg ttatgaggct tttgacgccg
     1141 ctattaagag agcaaagatc gaacttcctg acggacaatt aactcatgtg ctacgtcata
     1201 cgtttgccag ccattttatg atgcggggag gaaatattct tgtgttgcaa aaaatactgg
     1261 ggcatagcga tataaaaatg actatgcgtt atgcacactt tgcgccgggt catttagagg
     1321 ctgctgttga actgaatccc tttgacaata gagggtaaaa agtggcgatg aaaagtaaca
     1381 aaagcatcaa atagcgccat attgctttat ctgtatactt aataaaatca taacgttatg
     1441 atttataaat ggtggttgat agt
//
LOCUS       DAZFQD010000313         1418 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.320, whole genome shotgun sequence.
ACCESSION   DAZFQD010000313 DAZFQD010000000
VERSION     DAZFQD010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1418
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.320"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..875)
                     /locus_tag="ACHKTW_005279"
     CDS             complement(<1..875)
                     /locus_tag="ACHKTW_005279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6466116.1"
                     /translation="MSKKFTMTLLSSSLGGLLLGISSGVLAQELPPIKDSGLPFYSVL
                     KEADASHVTYGSTYRIYYNKSSGWGTMRYVLNGKDETLFNFDQEGNIIVLSKDNSIAY
                     TVHEPVLKDFARMAAGLRTSDKENGKHQVDEEEVRRIFNKVNNLSNTIINPEILKQSA
                     FIASGPRPEGDRLVQASAAGEFALAVGTGAGADKKFATSVGSWSAASGQQSVALGGGT
                     YAYADASTALGSVAFVDNTARYGTAVGNRAKVDKDATEGTALGAKATVTNKNSVALGA
                     NSRTTRDNEVYIGYEA"
     gene            complement(1239..>1418)
                     /locus_tag="ACHKTW_005280"
     CDS             complement(1239..>1418)
                     /locus_tag="ACHKTW_005280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO6466117.1"
                     /translation="NNDVGVTAEELIALSEAAEQAMFTKGMEIHVRQRTMKKELEKLT
                     SADEILAYRVGWAQE"
BASE COUNT          391 a          354 c          247 g          426 t
ORIGIN      
        1 gcttcataac cgatataaac ctcattgtca cgggttgtgc gtgaattagc tcctaatgca
       61 acactgtttt tatttgttac tgttgccttt gcccctaacg cagtaccttc tgttgcatct
      121 ttatcaactt tggctcggtt acctactgcc gtgccatatc tagccgtgtt atcaacaaat
      181 gcaacgcttc ccagggctgt agatgcatct gcataagcat atgttccccc acctaaagcc
      241 acactttgct gtccacttgc tgcagaccat gatcccactg atgttgcaaa ttttttatcc
      301 gctccggccc ctgtaccaac agccagagcg aattcgcccg ccgcactagc ctgaaccaaa
      361 cggtcacctt ccggacgtgg tcctgatgcg atgaaagccg attgttttaa tatttcagga
      421 ttaataatag tatttgatag attattaact ttattgaaga tccggcggac ctcctcctca
      481 tcaacctgat gcttaccgtt ttctttgtca gacgttctga ggcctgctgc catacgggca
      541 aagtctttta gcacaggctc atgaacggta tatgcaattg agttatcttt actcaatact
      601 ataatattac cctcctgatc gaaattaaaa agggtctcat cttttccgtt cagaacatat
      661 cgcattgttc cccaaccaga ggatttgtta tagtaaatac gataagtact accgtatgta
      721 acatgcgaag catctgcctc tttcagcact gaataaaaag gcaaaccaga atctttgata
      781 ggaggtaact cctgagctaa aacacctgag ctgatcccaa gcagaagccc acccagagaa
      841 gatgacagaa gtgtcattgt aaacttttta ctcattatgg atatcctgtt aaattatctg
      901 tatacaaatc agccgcaaca cctcacactc ataaagaaat atgattaatt acggctttat
      961 tcaggatatt ccgaaaataa atttaaaaat tagttttata aatttaggat ttgtcttaca
     1021 ttattctggg catacctgct cacgcatacg accacaccgc cggaacagat accagaaaaa
     1081 acttacccca gtcagacttg ctgtttcaca tctgcttaca ctgtaatttg tgaacaaaat
     1141 tacaacatca gaaaaaatta actattcagt taacatgaaa tatttacagg aggtatagtt
     1201 catgagggaa agaaatgttt cccccatatt tttatctgtt attcctgtgc ccagccaacc
     1261 ctgtatgcca gtatctcatc cgcactggtc agcttttcca gctctttctt catggtgcgc
     1321 tgccggacgt ggatttccat ccctttggtg aacatcgcct gctctgccgc ttcactcagc
     1381 gctataagct cttctgctgt cacccccaca tcattgtt
//
LOCUS       DAZFQD010000314         1385 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.321, whole genome shotgun sequence.
ACCESSION   DAZFQD010000314 DAZFQD010000000
VERSION     DAZFQD010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1385
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.321"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..1322)
                     /locus_tag="ACHKTW_005281"
     CDS             complement(<1..1322)
                     /locus_tag="ACHKTW_005281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element IS4 family transposase"
                     /protein_id="HFO6466118.1"
                     /translation="MHIGQALDLVSRYDSLRNPLTSLGDYLDPELISRCLAESGTVTL
                     RKRRLPLEMMVWCIVGMALERKEPLHQIVNRLDIMLPGNRPFVAPSAVIQARQRLGSE
                     AVRRVFTKTAQLWHNATPHPQWCGLTLLAIDGVFWRTPDTPENDAAFPRQTHAGNPAL
                     YPQVKMVCQMELTSHLLTAAAFGTMKNSENELAEQLIEQTGDNTLTLMDKGYYSLGLL
                     NAWSLAGEHRHWMIPLRKGAQYEEIRKLGKGDHLVKLKTSPQARKKWPGLGNEVTARL
                     LTVTRKGKVCHLLTSMTDAMRFPGGEMADLYSHRWEIELGYREIKQTMQLSRLTLRSK
                     KPELVEQELWGVLLAYNLVRYQMIKMAEHLKGYWPNQLSFSESCGMVMRMLMTLQGAS
                     PGRIPELMHDLASMGQLVKLPTRRERAFPRVVKERPWKYPTAPKKSQSV"
BASE COUNT          280 a          390 c          358 g          357 t
ORIGIN      
        1 actgactggc tctttttcgg ggctgtgggg tatttccagg gcctctcctt taccactctc
       61 gggaaggccc tttcccttct tgtcggtaat ttcacaagtt gtcccatact tgcaagatcg
      121 tgcatcagct ccggtatacg tcccggtgaa gcgccctgca atgtcatcag cattctcatc
      181 accattccgc atgattctga gaaactcagt tgattcggcc agtaaccttt cagatgttcc
      241 gccattttaa tcatctgata tctcaccaga ttataagcca gtaagacacc ccacagctct
      301 tgctccacaa gctccggctt tttacttctc agcgtcagcc tgctcagttg catcgtctgt
      361 tttatctccc tgtatcccag ttcgatttcc cagcgatgac tgtacagatc cgccatttct
      421 cctccgggga agcgcatggc gtccgtcatc gacgtcagca gatggcagac ttttcctttg
      481 cgcgtcacgg tcagcaggcg ggctgtcacc tcatttccca gtcccggcca cttttttcgt
      541 gcctgcgggc tggttttcag cttcaccaga tgatcgcctt tacccagttt tctgatctct
      601 tcatattgcg ctccctttct gagagggatc atccagtggc ggtgttctcc cgccaggctc
      661 caggcattta acagtcccag tgagtaataa cctttgtcca ttaacgtcag agtgttatcg
      721 ccggtttgtt ctataagttg ctcagcaagc tcattttcgc tgttcttcat cgtgccgaag
      781 gctgcagccg tcagcagatg gctggtcagt tccatctggc agaccatttt gacctgcggg
      841 tagagcgccg ggttcccggc atgtgtctgg cgggggaagg ctgcatcgtt ctctggtgta
      901 tccggtgtgc gccagaacac accatcgatg gccagcaggg tcaggccgca ccattgcgga
      961 tgcggcgtgg cgttatgcca gagctgcgct gttttcgtga acacgcggcg gacagcctca
     1021 cttcccaggc gctggcgggc ctgaataacg gcactggggg caacgaaggg gcgattgccc
     1081 ggcagcatga tgtccaggcg attcacaatc tggtgaagag gttctttacg ctcaagcgcc
     1141 atgccaacaa tacaccagac catcatttcg aggggaagac ggcgcttgcg tagcgttaca
     1201 gtacctgatt cggcaaggca acgagagatg agttcggggt cgaggtaatc ccccagagaa
     1261 gtcagtgggt tacgcagaga atcgtaacgg gataccaaat caagggcctg tccaatgtgc
     1321 ataaaaaaat ccggaaacga gtgagcgttt ccggattctt acacagccac tggatcggtc
     1381 aactg
//
LOCUS       DAZFQD010000315         1355 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.322, whole genome shotgun sequence.
ACCESSION   DAZFQD010000315 DAZFQD010000000
VERSION     DAZFQD010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1355
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.322"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(30..380)
                     /locus_tag="ACHKTW_005282"
     CDS             complement(30..380)
                     /locus_tag="ACHKTW_005282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4406 domain-containing protein"
                     /protein_id="HFO6466119.1"
                     /translation="MAVIYIAGPMTGYKDHNRTAFFTEAMRLAADGHVVLNPATLPEG
                     LSQQQYMSICIPMLMCADAIYLLEGWVDSAGARAEYVMALKLNMPVSFPENRAAGETV
                     AQLLHKSKATQATE"
     gene            complement(377..589)
                     /locus_tag="ACHKTW_005283"
     CDS             complement(377..589)
                     /locus_tag="ACHKTW_005283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6466120.1"
                     /translation="MIQESTVIRFTANGRQYEVDESLIDQGMTRQDSRNSEMHHIRLI
                     NGSHFCATNMEEVRVLTATRNTGGKA"
     gene            complement(586..822)
                     /locus_tag="ACHKTW_005284"
     CDS             complement(586..822)
                     /locus_tag="ACHKTW_005284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6466121.1"
                     /translation="MNSENNKELLRELRLIARAHEEAAKVIAAAGRPELVIASAISLR
                     ITMEAIRVIQDQESKIATLKKQLNEKSAGQRSQQ"
     gene            complement(858..>1355)
                     /locus_tag="ACHKTW_005285"
     CDS             complement(858..>1355)
                     /locus_tag="ACHKTW_005285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HFO6466122.1"
                     /translation="EIRAFADKANSTRTLLIHADFKDTLNSASHLFGMGNTLCVYCDP
                     PYLQFSGRDNFTAYGKPFDEDEHVRLRAALDRLSQETGGMTSITISNSDTPETRRIYR
                     GYRMNCIQAPRSVGARTRESAPEVIATLRQCDACGHHGGGYCPDCGPVTGDATYSVMF
                     TPAST"
BASE COUNT          250 a          359 c          368 g          378 t
ORIGIN      
        1 cgcgcaatca tgtttcgatc tccggtcggt tactcagtcg cctgtgtggc ttttgatttg
       61 tgaagtagct gcgcgacggt ttcgccggct gcccggtttt ccgggaatga cactggcatg
      121 ttcagtttga gtgccattac gtactccgcc cgcgcgccag ctgaatccac ccagccttcc
      181 agtaagtaaa tggcatcggc acacatgagc atcggaatac aaatgctcat gtactgctgc
      241 tggcttaatc cttccggcaa cgtggcaggg ttcagcacaa cgtgcccgtc tgccgcgagc
      301 ctcatcgcct ctgtgaaaaa cgccgttctg ttgtgatctt tgtatccggt cattggaccg
      361 gcgatataaa tcactgctat gctttacctc ccgtattgcg tgttgctgtc agcacgcgta
      421 cttcttccat gttcgtggcg cagaagtgcg aaccgttgat aaggcggatg tgatgcatct
      481 cagaattgcg tgagtcctgg cgtgtcatgc cctggtcaat caggctttcg tctacctcgt
      541 actgacggcc gttggctgtg aaccggataa cggttgattc ctggatcatt gctgactcct
      601 ttgcccggca gatttctcgt tgagctgctt tttaagggtg gcgattttac tttcctgatc
      661 ctgaatcacc cggattgctt ccatggtgat cctcagtgag attgctgaag cgataacgag
      721 ctctggtctt ccagcagctg cgataacttt tgctgcttcc tcatgtgcac gggcgatgag
      781 acgcaattcc cgcaaaagct ctttgttgtt ttcgctattc atttcctgac tccggtgtta
      841 ctgattcatc tgtttattca cgtactggca ggggtaaaca tcacgctgta agttgcatct
      901 cccgttacag gtccgcagtc cgggcagtag ccaccgccat ggtgaccgca ggcgtcgcac
      961 tgtctcaggg tggcgataac ctccggtgct gattcccggg ttcttgcgcc aacgcttcgt
     1021 ggtgcctgta tgcagttcat ccggtaaccc cgataaatgc gtcgtgtttc cggtgtgtcg
     1081 gaattggaga tggtgatgct ggtcatgccg cccgtttcct gtgaaaggcg gtcaagcgct
     1141 gccctcagac gaacgtgctc gtcttcatcg aatggttttc cgtaggcggt aaaattgtcc
     1201 cggccactga attgcaggta agggggatcg cagtacacgc aaagcgtgtt gcccatgccg
     1261 aacaggtgac tggcagagtt cagcgtatcc ttaaaatctg cgtggataag cagcgttcgg
     1321 gtgctgtttg ccttgtctgc aaatgcgcgg atttc
//
LOCUS       DAZFQD010000316         1327 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.323, whole genome shotgun sequence.
ACCESSION   DAZFQD010000316 DAZFQD010000000
VERSION     DAZFQD010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1327
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.323"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..318)
                     /locus_tag="ACHKTW_005286"
     CDS             complement(<1..318)
                     /locus_tag="ACHKTW_005286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3289 family protein"
                     /protein_id="HFO6466123.1"
                     /translation="MNNILLPPINIPCTLFETISLFDDFSADDMQYGDMVEQDFLSLG
                     LSDISAKVDPYRLIKYHFPGPGSINVAFSASSSGTKISQRECTDILFAEMKELAKMLS
                     FFGQ"
     gene            499..651
                     /locus_tag="ACHKTW_005287"
                     /pseudo
     CDS             499..651
                     /locus_tag="ACHKTW_005287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
BASE COUNT          427 a          202 c          251 g          447 t
ORIGIN      
        1 ttgtccaaaa aaggaaagca tttttgctag ttctttcatt tctgcaaata aaatatcagt
       61 gcattcacgt tgtgatattt ttgttccgga acttgaggca ctaaaagcaa cattaataga
      121 acctgggcca gggaaatggt atttaataag tctgtaagga tcaacttttg ctgagatatc
      181 gcttaggccg agtgagagaa agtcttgttc taccatatca ccatattgca tatcatcagc
      241 agaaaaatca tcgaataaac taatagtttc gaaaagtgta cagggaatat taattggcgg
      301 aagtaatatg ttattcattt gcatttgcct tggttatatt taagctcaat atcaattcga
      361 gaacaaatgc tagggtaaaa aaatatttaa aaaagaagta aacttcacat tttttcatta
      421 catctctgaa gagaacctcg ttaatgattg gtcttgctca ttgttaataa caaaatgttg
      481 aatttaccga ttaggaaact ttttcctacg cggattgcct ccagctatgg catgaacttt
      541 gagtttatgc cagaactgaa gtggggcttc ggctaccctg gcgcgattat ctttatgatc
      601 ctcgcgggcc tgacaccgta tctgtacttt aagcgtaaga actggctgta actatacgat
      661 gtcgctgtga gtaagttagt gtgctgacag cgacatttta gccgagggaa tcgcagataa
      721 cataattgtt gtgaataata tccatgccac gttgagttgg acgcttaatg actcacatct
      781 caccccttca attgctgcag agataataga aaaaagtgtc aagcccaatg aaatcaatac
      841 aaagtagatt gccttacatc cagtatctct tattcttttt gctccaatat tgatatttac
      901 ataggtatta ttaataacca gaataaaact caaagccagg ttgatatcat tcaaagttat
      961 taatattgac catgatgtta atgtatagat tatgaactga aagcgtgata aacgctcaag
     1021 cctggatggg tagaatatat ttctgatgga tgatttgaac atttttacat tgttttatat
     1081 ataattgttt tgaatatata ttacctgaat tatgtggttt gcatatatat atgcaatata
     1141 gcagtctttg cattgataac tatacagtag taatttagag tgtgtaagtt tttattgatg
     1201 gggtatattt taattaaata taattgaaat gtatttttat tctataaaca atatcttggg
     1261 tgaatggatt ctatatggga aatggtaact tttggcagtt gttcttgggt aaagaacctt
     1321 acagcaa
//
LOCUS       DAZFQD010000317         1321 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.324, whole genome shotgun sequence.
ACCESSION   DAZFQD010000317 DAZFQD010000000
VERSION     DAZFQD010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1321
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.324"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            219..>1321
                     /locus_tag="ACHKTW_005288"
     CDS             219..>1321
                     /locus_tag="ACHKTW_005288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage protease"
                     /protein_id="HFO6466124.1"
                     /translation="MKTENHPKDANRPSGLAVLSASLTASGDGWCQLLPAGRVKARDG
                     RPEKPAEGWLINESACNRMKAGLSALNQPLLIDYDHHSMNAQKNGFKAIAAGWVKPEN
                     IEWREGQGIFIKPEWTPQAQKHIDDREYAYLSAYMHYFADTGEPYLLRMASLTNDPGI
                     TGMNPVAALSADDLYVVSPSQEQHPMNEQLRQLLTALGLTVADGDEFTPELGTAALSA
                     LTGIKTRADAHDNLKTQVASLSAELETAKGTPAGGSIDLTKYVPIETYNALRTEYVAL
                     SAQHGSTTLEQVLDKAESEGRIFKSERGYLEQLGGQIGVAALSAQLDARQPVAALTGL
                     QTDTVTVPDKKTATAVLSAEDIAAAKLLGKTEA"
BASE COUNT          328 a          368 c          352 g          273 t
ORIGIN      
        1 cgaaatcaga aaacgcttta aaaaggccac agaaacgcct taattgttta aggtatgcaa
       61 atgcattacc ttaccccctt caggcgcgtg tcgtgatttt ctaacctgta tttaacgggc
      121 tttaaaatct gcgtgaagcg cctttgcatt tttccctgaa aatacttcct gaaatcccgc
      181 aaaacccgcc ctgaaaactc ccggcttatg ctgccggtat gaagacagaa aatcacccaa
      241 aagacgcaaa tcgcccctct ggtttagccg tgctttcagc ttcgctgact gcatccggcg
      301 acggatggtg tcagttactg ccagcaggca gggtaaaggc ccgtgacggt cgcccggaaa
      361 aaccggctga aggctggctg attaacgaat ccgcctgcaa ccggatgaaa gccgggcttt
      421 ccgccctgaa tcagccgtta ctgattgatt atgaccatca ctccatgaat gcgcagaaaa
      481 acggattcaa ggccattgcc gcaggttggg ttaagccgga aaacatcgaa tggcgtgagg
      541 gtcaggggat tttcattaaa cctgaatgga cgccgcaggc ccaaaaacat attgatgacc
      601 gtgaatatgc ctatctgtct gcgtatatgc attatttcgc tgacaccggg gaaccctacc
      661 tgttgcgcat ggcttccctg acgaatgatc caggtattac gggtatgaac cctgtcgcag
      721 cactcagtgc tgacgatctc tatgtagttt ccccttctca ggagcaacac cccatgaatg
      781 agcaattacg ccagttgctg acggcgctcg gtctgaccgt ggctgacggt gacgaattta
      841 cgccggagct gggcacggcg gcgctgtctg cactcaccgg gatcaaaacc cgtgcggatg
      901 cacacgacaa cctgaaaaca caggtcgcca gtctgtcggc agagctggaa accgcaaaag
      961 gcacaccggc tggcggcagt attgatctga cgaaatacgt gcccattgaa acgtataacg
     1021 ccctgcgtac cgaatacgtt gcgctgtcgg cgcagcacgg cagcaccacg ctggagcagg
     1081 tgctggacaa ggctgaatcc gaagggcgca tcttcaaaag tgaacgcggc tatctggaac
     1141 aactgggcgg gcagattggc gtggcggcac tttctgcaca gcttgacgcc cgccagccgg
     1201 ttgcggccct gaccggcctt cagaccgaca ccgtgaccgt gccggataaa aagactgcca
     1261 ccgctgtgct gtcggctgaa gatatcgcgg ccgccaaact gctgggtaaa accgaagccg
     1321 a
//
LOCUS       DAZFQD010000318         1315 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.325, whole genome shotgun sequence.
ACCESSION   DAZFQD010000318 DAZFQD010000000
VERSION     DAZFQD010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1315
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.325"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            77..358
                     /locus_tag="ACHKTW_005289"
     CDS             77..358
                     /locus_tag="ACHKTW_005289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6466125.1"
                     /translation="MTEAEILGLIRRVAGISQQVDEQATQPDSVTAENYARVVAEVMR
                     RDGIELNGVDMRNIRTRVLELLAYRRRVEMYREKEKITYHWKKPERLRR"
BASE COUNT          373 a          281 c          344 g          317 t
ORIGIN      
        1 aggtcgccgc cccgtaacgg gccataattc caggaaggac ccgacgacac cagactatca
       61 gaacgatggg ggcacaatga cagaagccga aatactggga ttaatccgcc gcgttgccgg
      121 aatcagccag caggttgacg aacaggccac gcagccggac agcgtgaccg ctgaaaatta
      181 tgcgcgtgtt gttgcggagg tgatgcgccg tgatggtata gaacttaatg gcgtggatat
      241 gcgcaacata cgaaccagag ttcttgagtt gctggcctac cgtcgccgtg tggagatgta
      301 tcgggagaag gagaaaataa cgtaccactg gaagaagccg gagcgtttgc ggcggtaact
      361 ggttgatatt cccgacggac caaaaatggg tggctacatc cctcgcttct gaggactgtt
      421 aacaagctgc ctgaaagaca gtcacaaact acgaaatttt cgcagtttaa ttaatgatca
      481 gcttaatcgc taacccgctg atattttcgg aaacctcaat ttgaggaagt cggcgcggta
      541 actcgctgaa ctttaagcaa agcgcaaaat tgcgcttgct gaataatcat tatgattacg
      601 cagatgatta aggaatgacc gaaggcggaa attcgcctgt ggttaatggg ggagttgcag
      661 atctgcaact cgaccatgaa actacggaaa ctacccgtag tttgggtagt aagagtaaca
      721 cccagatttt ggggcttact cgtgataccc aaatttgggg tatcggtggc aaccataacg
      781 actttcgtta cggttgatgc ttttacccca ttggtggagg ctgggcggcc tgctgtgggc
      841 ttttccacca ttggtggaaa ggtgcagatc tggatcatca ttacggtgat caagggtatg
      901 taaaacccac cagcctgatt aacagttaac cggaaaaaaa atccggcatg gggggggagt
      961 ggttagccac gaacaacgaa taagctggac accgcgcccc ctctcacgca gagaaaaaat
     1021 taccgtttcg tagtagttaa catgttaact gcctgatgaa gcgttgttgt tgattttttc
     1081 tttatgattc acctggttgc gtgcaaaaaa tgttaactac ctttttggta aaaatgttaa
     1141 cagggcagcg gttaacatta accaggcgta aatcccgata tggtgatccc catatcgggg
     1201 aacatccacc agcttaaaaa cgaatgagcg aaaaaaatcc aggtagaatt atggaagtcg
     1261 ctagggcaat cgccgtttaa attgtgcatt ttttcagcaa attattttag ttata
//
LOCUS       DAZFQD010000319         1313 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.326, whole genome shotgun sequence.
ACCESSION   DAZFQD010000319 DAZFQD010000000
VERSION     DAZFQD010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1313
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.326"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            220..>1313
                     /locus_tag="ACHKTW_005290"
     CDS             220..>1313
                     /locus_tag="ACHKTW_005290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage protease"
                     /protein_id="HFO6466126.1"
                     /translation="MKTKNTPKLAYAILNAISLSADGDGDWCQIMPAGRVKARDGRPE
                     KPAEGWLINRATVERMVSRVVALNQPVKIDYNHQTLIKGHQAPAAGFVTASPENFRFS
                     EERGFEVRPKWNPPALEHLRNNEFPWFSPVIGYDENTGEPVELRMLAITGDPGLTGMN
                     PVAALSADDLYNALNPPLKDTSMNEQLRQLLTALGLTVADGDEFTPELGTAALSALTG
                     IKTRADAHDNLKTQVASLSAELETAKGTPAGGSIDLTKYVPIETYNALRTEYVALSAQ
                     HGSTTLEQVLDKAESEGRIFKSERGYLEQLGGQIGVTALSAQLDARQPVAALTGLQTD
                     TVTVPDKKTATASLSAEDIAAAHLLGKTEA"
BASE COUNT          310 a          379 c          355 g          269 t
ORIGIN      
        1 cgaaatcaga aaacgcttta aaaaggccac agaaacgcct taattgttta aggtatgcaa
       61 atgcattacc ttaccccctt caggcgcgtg tcgtgatttt ctaacctgta tttaacgggc
      121 tttaaaatct gcgtgaagcg cctttgtatt tttcccctgg aagcacttcc tgaaatcccg
      181 caaaacccgc cctgaaaatt cccggcttat gctgccggaa tgaagacgaa aaacacgccc
      241 aaacttgctt atgccattct gaatgccatc agcctgtccg cagacgggga cggtgactgg
      301 tgtcagatca tgccagccgg tcgggtaaag gcccgtgacg gtcgcccgga aaaaccggct
      361 gaaggctggc tgattaaccg tgcgaccgtt gagcgcatgg tatcccgggt tgtggcgctc
      421 aatcagccgg taaaaattga ctacaaccac cagaccctga ttaaggggca tcaggcaccg
      481 gctgccggtt ttgtgacggc ttcaccggaa aacttccgct tcagtgaaga gcgtggcttc
      541 gaggtgcgcc cgaagtggaa ccctccggcc cttgaacatc tgcgtaataa cgaattcccc
      601 tggttttcac cggtgattgg ctatgacgag aacaccggcg aacctgtcga acttcggatg
      661 ctggctatta ccggtgaccc cggtctgacc ggcatgaatc ctgtcgccgc gctgtcggcg
      721 gatgaccttt ataacgcctt aaaccctcct ttaaaggata cctccatgaa tgagcaatta
      781 cgccagttgc tgacggcgct cggtctgacc gtggctgacg gtgacgaatt tacgccggag
      841 ctgggcacgg cggcgctgtc tgccctcacc gggatcaaaa cccgtgcgga tgcacacgac
      901 aacctgaaaa cacaggtcgc cagtctgtcg gcagagctgg aaaccgcaaa aggtacaccg
      961 gctggcggca gtattgatct gacgaaatat gtgcccattg aaacgtataa cgccctgcgt
     1021 accgaatacg ttgcgctgtc agcacagcac ggcagcacca cgctggagca ggtgctggac
     1081 aaggccgagt ccgaagggcg catcttcaaa agcgaacgcg gctatctgga acagctgggt
     1141 gggcagattg gtgttactgc actttctgca cagcttgacg cccgacagcc ggttgcagcc
     1201 ctgaccggcc ttcagaccga caccgtgacc gtgccggata aaaagaccgc caccgcctcg
     1261 ctgtcggctg aagatatcgc cgccgcccat cttctgggta aaaccgaagc cga
//
LOCUS       DAZFQD010000320         1291 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.327, whole genome shotgun sequence.
ACCESSION   DAZFQD010000320 DAZFQD010000000
VERSION     DAZFQD010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1291
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.327"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            32..1144
                     /locus_tag="ACHKTW_005291"
     CDS             32..1144
                     /locus_tag="ACHKTW_005291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element IS421 family transposase"
                     /protein_id="HFO6466127.1"
                     /translation="MNYSHDNWSAILAHIGKPEELDTSARNAGALTRRREIRDAATLL
                     RLGLAYGPGGMSLREVTAWAQLHDVATLSDVALLKRLRNAADWFGILAAQTLAVRAAV
                     TGCTSGKRLRLVDGTAISAPGGGSAEWRLHMGYDPHTCQFTDFELTDSRDAERLDRFA
                     QTADEIRIADRGFGSRPECIRSLAFGEADYIVRVHWRGLRWLTAEGMRFDMMGFLRGL
                     DCGKNGETTVMIGNSGNKKAGAPFPARLIAVSLPPEKALISKTRLLSENRRKGRVVQA
                     ETLEAAGHVLLLTSLPEDEYSAEQVADCYRLRWQIELAFKRLKSLLHLDALRAKEPEL
                     AKAWIFANLLAAFLIDDIIQPSLDFPPRSAGSEKKN"
BASE COUNT          323 a          314 c          352 g          302 t
ORIGIN      
        1 ggtattacgc ctgatatgat ttaacgtgcc gatgaattac tctcacgata actggtcagc
       61 aattctggcc catattggta agcccgaaga actggatact tcggcacgta atgccggggc
      121 tctaacccgc cgccgcgaaa ttcgtgatgc tgcaactctg ctacgtctgg ggctggctta
      181 cggccccggg gggatgtcat tacgtgaagt cactgcatgg gctcagctcc atgacgttgc
      241 aacattatct gacgtggctc tcctgaagcg gctgcggaat gccgccgact ggtttggcat
      301 acttgccgca caaacacttg ctgtacgcgc cgcagttacg ggttgtacaa gcggaaagag
      361 attgcgtctt gtcgatggaa cagcaatcag tgcgcccggg ggcggcagcg ctgaatggcg
      421 actacatatg ggatatgatc ctcatacctg tcagttcact gattttgagc taaccgacag
      481 cagagacgct gaacggctgg accgatttgc gcaaacggca gacgagatac gcattgctga
      541 ccggggattc ggttcgcgtc ccgaatgtat ccgctcactt gcttttggag aagctgatta
      601 tatcgtccgg gttcactggc gaggattgcg ctggttaact gcagaaggaa tgcgctttga
      661 catgatgggt tttctgcgcg ggctggattg cggtaagaac ggtgaaacca ctgtaatgat
      721 aggcaattca ggtaataaaa aagccggagc tccctttccg gcacgtctca ttgccgtatc
      781 acttcctccc gaaaaagcat taatcagtaa aacccgactg ctcagcgaga atcgtcgaaa
      841 aggacgagta gttcaggcgg aaacgctgga agcagcgggc catgtgctat tgctaacatc
      901 attaccggaa gatgaatatt cagcagagca agtggctgat tgttaccgtc tgcgatggca
      961 aattgaactg gcttttaagc ggctcaaaag tttgctgcac ctggatgctt tgcgtgcaaa
     1021 ggaacctgaa ctcgcgaaag cgtggatatt tgctaatcta ctcgccgcat ttttaattga
     1081 cgacataatc cagccatcgc tggatttccc ccccagaagt gccggatccg aaaagaagaa
     1141 ctaactcgtt gtggagaata acaaaaatgg tcatctggag cttacaggtg gccattcgtg
     1201 ggacagtatc cctgacagcc tacaaaacgc aattgaagaa cgcgaggcat cgtcttaacg
     1261 aggcaccgag gcgtcgcatt cttcagatgg t
//
LOCUS       DAZFQD010000321         1283 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.328, whole genome shotgun sequence.
ACCESSION   DAZFQD010000321 DAZFQD010000000
VERSION     DAZFQD010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1283
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.328"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            106..1188
                     /locus_tag="ACHKTW_005292"
     CDS             106..1188
                     /locus_tag="ACHKTW_005292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpD"
                     /protein_id="HFO6466128.1"
                     /translation="MKKLTVAISAVAASVLMAMSAQAAEIYNKDSNKLDLYGKVNAKH
                     YFSSNDADDGDTTYARLGFKGETQINDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAF
                     AGLKFGDYGSIDYGRNYGVAYDIGAWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNN
                     DFFGLVDGLNFAAQYQGKNDRTDVTEANGDGFGFSTTYEYEGFGVGATYAKSDRTNNQ
                     VIYGNNSLNASGQNAEVWAAGLKYDANNIYLATTYSETQNMTVFGNNHIANKAQNFEV
                     VAQYQFDFGLRPSVAYLQSKGKDLGAWGDQDLVEYIDVGATYYFNKNMSTFVDYKINL
                     IDKSDFTKASGVATDDIVAVGMVYQF"
BASE COUNT          376 a          271 c          280 g          356 t
ORIGIN      
        1 acgagaaacc aaaacttaca tcttgaaata atcacattga ttagatgaat atttatcgcg
       61 cagtgacatc attttttaat aatagttcaa aaaaaagggc tcacgatgaa aaaattaaca
      121 gtggcaattt ctgctgtagc tgcatcagta ctgatggcga tgtctgctca ggcagctgaa
      181 atttataata aagacagtaa caagctggat ctatatggga aagttaatgc caagcactac
      241 ttctcctcta atgatgcaga tgatggtgat actacttatg cccgtcttgg cttcaaaggt
      301 gaaacccaaa tcaacgatca actgactggt ttcggtcagt gggaatatga attcaaaggc
      361 aaccgcgctg aatctcaagg ctcctccaaa gacaaaaccc gtcttgcatt tgcaggcctg
      421 aaattcgggg actacggctc aatcgattac ggccgtaact acggtgtagc atatgacatc
      481 ggtgcgtgga ctgacgttct gccagaattc ggtggcgata cctggaccca aacagatgtg
      541 ttcatgactg gtcgcaccac tggtgttgca acttatcgta acaacgactt ctttggtctg
      601 gttgatggtc tgaactttgc tgctcagtat cagggtaaaa atgaccgcac tgacgtaact
      661 gaagctaatg gtgatggttt cggtttctcc actacttatg agtatgaagg attcggtgta
      721 ggtgcaacct atgctaaatc tgaccgcact aataatcagg ttatctacgg taacaacagc
      781 ctgaatgcat ctggtcaaaa tgctgaagta tgggcagctg gtctgaaata tgatgcgaac
      841 aacatctatc tggctaccac ctattctgaa acccagaaca tgactgtttt tggtaataac
      901 catattgcca acaaagcaca aaacttcgaa gtagttgcac aatatcagtt cgacttcggt
      961 ctgcgtccat ccgttgctta cctgcaatct aaaggaaaag acttgggtgc gtggggtgat
     1021 caggacctgg ttgaatatat tgatgtaggt gcaacctatt acttcaacaa aaatatgtcc
     1081 acttttgttg attacaaaat caacctgatt gataagagcg atttcacgaa agcatctggc
     1141 gttgctaccg atgatatcgt tgctgtaggt atggtttacc agttctaatt tgattactaa
     1201 aagatatgtt gcgggaggct ttgcctcccc aacatataag tggctccctc aagccacttc
     1261 ctttaggagc acaaccttgc ttc
//
LOCUS       DAZFQD010000322         1250 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.329, whole genome shotgun sequence.
ACCESSION   DAZFQD010000322 DAZFQD010000000
VERSION     DAZFQD010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1250
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.329"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>1250
                     /locus_tag="ACHKTW_005293"
     CDS             <1..>1250
                     /locus_tag="ACHKTW_005293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HFO6466129.1"
                     /translation="GQTPSWYYVVVIAGQSNASSFGEGLPLPDTYDRPDPRIKQLARR
                     NTVTPGGVECAYNDIIPADHCLHDVLDMSNHNHPKADLSKGQYGCVGQGLHIAKKLLP
                     FIPEEAGILLVPCARGGSAFTDGADGEFTEASGATSASSRWGVNKPLFSDLVNRTKAA
                     LSSNPRNILLSVVWMQGENDLKTGKHAEHSGLFVTMVETFRRELSAFSAQSPAGDMAS
                     VPWICGDTTYYWKNTYPEQYKAVYGKYANEAKNIHFVPFVVDENGKNVPTNNPDEDPD
                     IPGIGYYGAISRDSTNWTSNDRASHFSSWARRGIISDRLATAILTLSGQKSAFFRGVT
                     ATEHSDTGKSEPEPTGDVKDVSDQMKIIVSYTANAPTEPMKDQGWNAASGKATFVTDA
                     AATGGNKALRVNKAPNSMASWKMY"
BASE COUNT          345 a          276 c          309 g          320 t
ORIGIN      
        1 ggtcaaactc cgtcctggta ttacgtggtt gttattgctg gtcagtcgaa tgcatcgtca
       61 tttggtgaag gtcttccttt gcctgatacc tatgatcgac ctgatccacg tattaaacag
      121 ctggctcgcc gtaacacggt tacacctggc ggggttgaat gcgcctacaa cgatataatt
      181 ccggcagatc actgtcttca tgatgtgctg gatatgagta accataatca tccaaaagca
      241 gatctttcaa aaggtcagta tggttgtgtc ggccagggtt tgcatattgc aaaaaaactc
      301 ctgcccttta tcccggaaga ggctggtatc cttctggttc cctgtgccag aggaggctct
      361 gcatttaccg atggtgctga tggtgaattt actgaagctt ccggagcaac atctgcatca
      421 tcgcgctggg gagtcaataa acctttgttc agcgatcttg ttaaccgaac aaaagcggct
      481 ctcagcagta accccagaaa tattctcctg tccgttgtct ggatgcaggg cgagaatgat
      541 ctgaaaacag gaaaacatgc tgaacattcc ggtctgtttg tgacgatggt cgaaacattc
      601 aggcgtgaac tttcggcttt ttctgcgcaa tctccagccg gagatatggc ttctgtaccg
      661 tggatctgtg gtgatacgac gtattactgg aaaaatacat atccagaaca atacaaagct
      721 gtttatggca aatatgctaa tgaagcaaag aacattcact ttgttccatt tgttgtggat
      781 gaaaatggaa aaaatgtacc gacaaataat ccagatgaag acccggatat tccaggtatt
      841 ggctattatg gtgccataag tcgcgattcg actaactgga catcaaacga cagggcaagc
      901 catttcagtt catgggcgcg ccgtggaatt atttcagacc gtctggcaac agcgattctg
      961 acattgtcag gacaaaaatc tgctttcttc cgcggtgtaa cggcaacaga acattctgat
     1021 accggtaaga gtgagcctga acccaccggg gatgtgaagg acgtgagcga tcagatgaag
     1081 atcatcgttt cgtatactgc aaacgccccg acagaaccaa tgaaagatca ggggtggaat
     1141 gcggcttccg gaaaagcaac ctttgtcacg gatgctgctg caacaggtgg taacaaagcg
     1201 ttaagggtga ataaagcgcc taacagcatg gcctcatgga aaatgtatca
//
LOCUS       DAZFQD010000323         1241 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.330, whole genome shotgun sequence.
ACCESSION   DAZFQD010000323 DAZFQD010000000
VERSION     DAZFQD010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1241
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.330"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>1241
                     /locus_tag="ACHKTW_005294"
     rRNA            <1..>1241
                     /locus_tag="ACHKTW_005294"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          312 a          287 c          392 g          250 t
ORIGIN      
        1 ctgagaggat gaccagccac actggaactg agacacggtc cagactccta cgggaggcag
       61 cagtggggaa tattgcacaa tgggcgcaag cctgatgcag ccatgccgcg tgtatgaaga
      121 aggccttcgg gttgtaaagt actttcagcg gggaggaagg gagtaaagtt aatacctttg
      181 ctcattgacg ttacccgcag aagaagcacc ggctaactcc gtgccagcag ccgcggtaat
      241 acggagggtg caagcgttaa tcggaattac tgggcgtaaa gcgcacgcag gcggtttgtt
      301 aagtcagatg tgaaatcccc gggctcaacc tgggaactgc atctgatact ggcaagcttg
      361 agtctcgtag aggggggtag aattccaggt gtagcggtga aatgcgtaga gatctggagg
      421 aataccggtg gcgaaggcgg ccccctggac gaagactgac gctcaggtgc gaaagcgtgg
      481 ggagcaaaca ggattagata ccctggtagt ccacgccgta aacgatgtcg acttggaggt
      541 tgtgcccttg aggcgtggct tccggagcta acgcgttaag tcgaccgcct ggggagtacg
      601 gccgcaaggt taaaactcaa atgaattgac gggggcccgc acaagcggtg gagcatgtgg
      661 tttaattcga tgcaacgcga agaaccttac ctggtcttga catccacgga agttttcaga
      721 gatgagaatg tgccttcggg aaccgtgaga caggtgctgc atggctgtcg tcagctcgtg
      781 ttgtgaaatg ttgggttaag tcccgcaacg agcgcaaccc ttatcctttg ttgccagcgg
      841 tccggccggg aactcaaagg agactgccag tgataaactg gaggaaggtg gggatgacgt
      901 caagtcatca tggcccttac gaccagggct acacacgtgc tacaatggcg catacaaaga
      961 gaagcgacct cgcgagagca agcggacctc ataaagtgcg tcgtagtccg gattggagtc
     1021 tgcaactcga ctccatgaag tcggaatcgc tagtaatcgt ggatcagaat gccacggtga
     1081 atacgttccc gggccttgta cacaccgccc gtcacaccat gggagtgggt tgcaaaagaa
     1141 gtaggtagct taaccttcgg gagggcgctt accactttgt gattcatgac tggggtgaag
     1201 tcgtaacaag gtaaccgtag gggaacctgc ggttggatca c
//
LOCUS       DAZFQD010000324         1241 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.331, whole genome shotgun sequence.
ACCESSION   DAZFQD010000324 DAZFQD010000000
VERSION     DAZFQD010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1241
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.331"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..554
                     /locus_tag="ACHKTW_005295"
     CDS             <1..554
                     /locus_tag="ACHKTW_005295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO6466130.1"
                     /translation="MAEVQAAKTKAEEAATRAAVPGPKGERGEPGAPGPKGDAGPAGP
                     AGKDGTAGAEGKAGPAGPRGERGPAGAQGVPGPVGPAGPAGKTGPQGPAGERGPMGPP
                     GPAGDAGNGVIKGIRLGNESVFRPHEGYINWDIRLTDGSVLTGVVGMNASGTNRINEI
                     YYRPLQITTDGVYWRTIGIDNGM"
     gene            904..>1241
                     /locus_tag="ACHKTW_005296"
     CDS             904..>1241
                     /locus_tag="ACHKTW_005296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000547696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFO6466131.1"
                     /translation="MIIKKFTLYSPEKADVPGAMYLKSEDGRDWYESQSLFSADTLKV
                     AYDSNGIICGISKDVSVMWPVNMSVAEVPDSEENRKADSSGRWKFDGERIVDTLTAEK
                     ARGLKGDEINA"
BASE COUNT          339 a          254 c          387 g          261 t
ORIGIN      
        1 taatggcaga agtgcaggca gcaaaaacga aggcagaaga ggcagcaacc cgggcagcag
       61 tccccggacc gaagggggag aggggggaac ctggcgcacc tggtcccaaa ggtgatgcag
      121 gcccggcagg ccccgcagga aaggatggta ccgccggagc ggaaggtaag gcaggtccgg
      181 cagggccacg aggtgaaagg ggacccgcgg gggcacaggg tgttccgggg ccggttggtc
      241 cggcaggtcc tgcgggtaaa acggggccac agggccctgc cggagagcga gggccgatgg
      301 gaccgcccgg gccagcaggt gatgcaggga atggcgttat aaaaggcatt cggctgggaa
      361 atgagagcgt gtttcgtcca catgaaggct atatcaactg ggatatacgc ttgacggatg
      421 gcagtgtgct tacgggagtg gtgggaatga atgcgtcagg gacaaaccgg ataaatgaaa
      481 tctattacag gccgttacag ataacaacag atggcgttta ctggcgtact attggcattg
      541 ataatggtat gtgagcagat tataccatcc ttaccggatg agatataaaa gttaaaagag
      601 gaaatgtcgg gttagcatct ttctgattcg tccggctgat atcaccacgc catatgttta
      661 cgctgaacat ggcggataaa aagacggggg agcaggcagc agactcccct gaaaggtatc
      721 cctgtcattt ttacattttt attctgtgtc acggttacac atattttccc gaataaccac
      781 tactgatact taccgtattc tggtgattat ttttgtgatg tgaactgcat tttatttata
      841 aagagcaaac agattaacag ccgcgcgttt tactgtgcag tgctgcgaaa gggtaacgga
      901 tttatgatta ttaaaaaatt cacgctgtat tccccggaga aggcagatgt ccccggggca
      961 atgtatctga agtcagaaga cggcagggac tggtatgaaa gccagtcttt attttcagca
     1021 gatacgctga aggtggctta tgacagtaac ggaataatct gcggtataag caaggatgta
     1081 tctgttatgt ggcctgttaa catgagtgtt gcagaagtgc ctgattcgga ggaaaaccgc
     1141 aaggcagaca gctcagggcg ctggaagttt gacggggagc gaatagtcga cacactgaca
     1201 gcggagaaag cacgcgggct gaagggagat gaaatcaacg c
//
LOCUS       DAZFQD010000325         1232 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.332, whole genome shotgun sequence.
ACCESSION   DAZFQD010000325 DAZFQD010000000
VERSION     DAZFQD010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1232
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.332"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>1232
                     /locus_tag="ACHKTW_005297"
     CDS             <1..>1232
                     /locus_tag="ACHKTW_005297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFO6466132.1"
                     /translation="GFTAGAEGAFNEASGATAGSSLWGADKPLYHDLVSRTRAALKKN
                     PKNVLLSVIWMQGEKDVSSGRHAEHNALFLAMVNQFRTELADVAEQCTGGTTASVPWI
                     CGDTTYYWKERYAAPYEAVYGGYKGKAAQNIHFVPLMTDEHGVNVPTNEPSEDPDIIP
                     AGYYGAASRTASNWTAADRKTHFSSWARRGLVSDRLAGAILQYAGRTLSFLTGQSAPQ
                     SGGTTPVSPGTPDVEKPQDGGVAGTGHDEAVSSTRTVAEYDANSGNGALTEQQWGASG
                     GKGTVTDDGGRKALRLEKQPGKLTSWKMFRTIAVEEAKNLLSKGGEIAVRFKIPDGSE
                     LVNGQFVFGIYWPVSQWASGATANSMLASFFLQTDTSNLNLMHQKGSSNAQLGTFGTF
                     DHNWHTVVFRFAGNNSER"
BASE COUNT          301 a          278 c          397 g          256 t
ORIGIN      
        1 cgggatttac tgcgggggcc gagggggcgt ttaacgaagc gtcgggagcg accgcaggct
       61 cgtccctgtg gggggcggat aaaccgctgt atcatgacct ggtcagcaga acccgtgcgg
      121 ccctgaagaa aaatccgaag aacgtgctgt tatcagtgat ctggatgcag ggagaaaaag
      181 atgtcagttc ggggagacat gcagaacaca atgcgctttt ccttgccatg gtaaatcagt
      241 tccgtacgga actggcagat gtggcagagc agtgtactgg cgggacaacg gccagcgtcc
      301 cgtggatttg cggtgatacc acgtactact ggaaggaaag gtatgcagcg ccgtatgagg
      361 cggtttacgg tgggtataaa ggaaaagctg cgcagaatat tcactttgtg ccgttgatga
      421 cggatgagca tggtgtgaat gtgccgacaa acgagccgtc agaagatccg gacattatcc
      481 cggcgggata ctatggcgct gcgtcacgca ctgccagtaa ctggacggca gccgatcgta
      541 aaacgcactt cagttcatgg gcgcgaagag gccttgtttc agaccgcctg gcaggtgcca
      601 ttttgcagta cgccgggcgg acattatcgt tccttaccgg gcagagcgca ccgcagtcgg
      661 gtggtacaac gcctgtcagt ccggggacac cggatgttga gaaaccgcag gatggcggtg
      721 ttgctggtac tggtcatgat gaggccgtga gcagtaccag gacggtggct gaatatgatg
      781 cgaacagtgg aaatggtgca ttgacagagc agcagtgggg cgcttccggc ggcaaaggca
      841 ctgtgactga tgacggtgga cggaaagcgc tgcgactgga aaaacagccc ggtaaactga
      901 cgtcctggaa aatgttccgt actattgcag tggaggaggc aaaaaatctt ctcagcaagg
      961 ggggggaaat tgccgtacgg ttcaaaattc cggatggaag cgagctggtg aacggacagt
     1021 ttgtctttgg tatctactgg ccggtgtcgc agtgggcgtc aggcgcgaca gcaaacagta
     1081 tgctggcatc attcttcctt cagacggata catcaaatct taatctgatg caccagaagg
     1141 gctcctcgaa tgcacaactg ggtacatttg gtacgtttga ccataactgg catacggttg
     1201 ttttccgctt tgcgggaaat aacagcgaaa ga
//
LOCUS       DAZFQD010000326         1220 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.333, whole genome shotgun sequence.
ACCESSION   DAZFQD010000326 DAZFQD010000000
VERSION     DAZFQD010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1220
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.333"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..1115)
                     /locus_tag="ACHKTW_005298"
     CDS             complement(<1..1115)
                     /locus_tag="ACHKTW_005298"
                     /EC_number="2.4.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylglucosaminyltransferase"
                     /protein_id="HFO6466133.1"
                     /translation="MVDKIIFTVTPIFSIPPRGAAAVETWMYQVAQRTNFPNRIVCIK
                     NPGYSNYTFVNDNCSIHRVGFSRIYKRLFQKWTRLDPLPYSQRILNIAHDFPITKESV
                     IVVHNSMKLYTQIRKRAPQARVVIHMHNAFEPKLLEQNVKMIVPSLYLKKYYQSYLAN
                     ADIEIVPNGIDLETYQSNFQPITRSELNISPEEKIIFYAGRIVPDKGILLLMQSFEKL
                     AAAHKNLKLVVIGDYTEMSKSDKGAYQRNVREIAKRLKDRCIMLGSIPPEKMHCYYPL
                     ADLVVIPSQFQEPFCMVAIEAMGAGKPVLVSTRGGMTEFVKENTTGFHLKEPMTADSI
                     SSDILKTLANPELTAVAKQGQDFVFEHYSWDGVTQRF"
     gene            complement(1124..>1220)
                     /locus_tag="ACHKTW_005299"
     CDS             complement(1124..>1220)
                     /locus_tag="ACHKTW_005299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008918 - lipopolysaccharide
                     3-alpha-galactosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 8 C-terminal
                     domain-containing protein"
                     /protein_id="HFO6466134.1"
                     /translation="HEYKEKYKHLLYQKKFLDGVFTAIKYNVMKG"
BASE COUNT          371 a          243 c          201 g          405 t
ORIGIN      
        1 tcgaatcttt gagttacgcc atcccaactg tagtgttcaa acacaaagtc ctgaccttgc
       61 tttgcaactg ctgttaattc aggattcgct aacgttttta aaatatcgct gcttatggag
      121 tctgcagtca taggttcttt taaatggaat cccgtagtat tttctttgac aaattccgtc
      181 atccccccac gcgtactcac taaaacaggc ttaccagcac ccatagcttc aattgccacc
      241 atgcaaaatg gttcctggaa ctgggaagga atgacgacta aatctgccag tgggtaataa
      301 caatgcatct tttcaggcgg tatgcttcct aacataatgc aacgatcctt caaacgtttt
      361 gcaatttccc tgacattgcg ttgatatgca cctttgtcac ttttactcat ttctgtataa
      421 tcaccaatca caactaattt taaatttttg tgagcagcgg ccaatttttc gaatgattgc
      481 atcaacagca atatgccttt gtcagggacg atacgcccag cataaaaaat aattttctct
      541 tcaggggaga tattaagttc gcttctagtg atcggctgaa aattagattg ataggtttct
      601 aaatcaattc catttggtac aatctcaata tctgcatttg ctaaataaga ttggtaatac
      661 tttttcaagt ataaactagg cactatcatt ttaacatttt gctcaagcaa ttttggttca
      721 aatgcattat gcatatgtat aaccactctt gcctgaggag ctcgcttacg aatttgagtg
      781 tatagcttca tgctattatg aactacaata acactttctt tcgttatagg aaaatcatgt
      841 gcaatattca atattctctg tgaatatgga agaggatcaa gtcttgtcca tttttgaaat
      901 aaccgcttat atatgcgact gaatccaacc ctatgaatac tacaattatc attaacaaag
      961 gtgtaatttg agtatcctgg atttttgata caaacaatac gatttggaaa attggtacgc
     1021 tgagcaacct gatacatcca cgtttcaaca gcagccgctc cgcgaggagg aattgaaaaa
     1081 ataggagtaa ccgtaaatat tattttatca accatctcgt atattaacct ttcataacat
     1141 tatatttaat tgctgtaaaa acaccatcga gaaatttttt ctggtaaagc aagtgtttat
     1201 atttttcttt gtactcatgt
//
LOCUS       DAZFQD010000327         1214 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.334, whole genome shotgun sequence.
ACCESSION   DAZFQD010000327 DAZFQD010000000
VERSION     DAZFQD010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1214
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.334"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..62)
                     /locus_tag="ACHKTW_005300"
     CDS             complement(<1..62)
                     /locus_tag="ACHKTW_005300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6466135.1"
                     /translation="MGNRKQVTSRIISTPELIRY"
     gene            complement(151..216)
                     /locus_tag="ACHKTW_005301"
                     /pseudo
     CDS             complement(151..216)
                     /locus_tag="ACHKTW_005301"
                     /inference="COORDINATES: protein motif:HMM:NF021626.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Com family DNA-binding transcriptional
                     regulator"
     gene            331..552
                     /locus_tag="ACHKTW_005302"
                     /pseudo
     CDS             331..552
                     /locus_tag="ACHKTW_005302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313023.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            575..685
                     /locus_tag="ACHKTW_005303"
                     /pseudo
     CDS             575..685
                     /locus_tag="ACHKTW_005303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313023.2"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(872..1072)
                     /locus_tag="ACHKTW_005304"
     CDS             complement(872..1072)
                     /locus_tag="ACHKTW_005304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6466136.1"
                     /translation="MDKNKKQPDTKGLEILSAEIINALRSGLDENTLQDRNFTTAMWI
                     AMQILCTPRCAIFAVREGQLTR"
BASE COUNT          319 a          257 c          277 g          361 t
ORIGIN      
        1 ttatagcgaa taagttcagg cgtgctgatg atacgggatg tgacttgctt tctgttcccc
       61 acgacaggat ttccttgtgt atgtggggtg ctctgtggcg ctcggagatg tgatttgatt
      121 gagggtttta cagcgcggac attttacttc cagataactg aaactggccc gtgccagtag
      181 cttattgcag tgtcggcacc gtatgtttcg atacatggcc gcataacctc ctgctttgtt
      241 gttgatgcct ttctcatgca atcgatcgac aaaaacgatc gattcgattt gtttaattga
      301 tacaacaaat agattgaaaa gtcatcaaca atgattcagg gcggtgatta tttacttaaa
      361 gaaaacgaag agttaggtgg tgaaaacgat cataaaatga tcattttatt gcctttttat
      421 ccagtgaagg taggatcgcg cgtatatgta gtatgtttca aatcaacagg atactacata
      481 aaagggaaac ccagcatagc tggaactatg ctgggccgat ttagacaagt ttgcatcaat
      541 gctttacttc gggactacat aaatggcgac tcagatagcg cgaactattc tgggtcaatc
      601 tgtaaaaaac tgcatcaatg cttcactctg agctcgtggt gtatcctatg atattccccg
      661 gcctcctgta aagcagagat gcatctgttc aggcaggaga aaatggataa actgaccacc
      721 gaccaaaagt tacaggctgc gcgaattgca gctgatatca caattgcagc aatgacatcc
      781 accagttgtg tctttacctc ttttcataca gctggtgatt catcagctat gcaattatgg
      841 aacgcgtgct tttctgatgt aattcgggca atcagcgagt caactgaccc tcacgaaccg
      901 caaaaatagc gcatcttggg gtacacagga tctgcatcgc tatccacatt gcagtggtga
      961 agtttcgatc ctgtaatgta ttttcatcaa gtcctgaacg tagcgcattg ataatttctg
     1021 cgcttaatat ttccagacct ttggtatcgg gctgcttttt gttcttgtcc atgattattt
     1081 cctctgactg gtatatacat cgcccatgaa aatcaaaccg acaggagaag ctgtcggttt
     1141 gtattgttac atcatggcac tgtcagttcc gggctgaacc ggctctggtt gctctacggg
     1201 ctgaggctca ttct
//
LOCUS       DAZFQD010000328         1212 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.335, whole genome shotgun sequence.
ACCESSION   DAZFQD010000328 DAZFQD010000000
VERSION     DAZFQD010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1212
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.335"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..252
                     /locus_tag="ACHKTW_005305"
     CDS             <1..252
                     /locus_tag="ACHKTW_005305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6466137.1"
                     /translation="VTLIYFLPGEDSFYSAPYEYSRGSSKSCSGAFVDDPDLQKTIFI
                     CYPYGDYQDGNVIYVKKRVNALGAVVTYAYATSGRFRFD"
     gene            complement(302..619)
                     /locus_tag="ACHKTW_005306"
     CDS             complement(302..619)
                     /locus_tag="ACHKTW_005306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFO6466138.1"
                     /translation="MPTQVATEGRLAADPNTRRTTKGTNVTTAQLAVSLPCHGAEQPT
                     TMWLYLIAFGKQHEELAKHRKGDLILVHGNLQVNRYQDKYGQQKEGWQLLAQSIISSR
                     TGR"
     gene            complement(652..990)
                     /locus_tag="ACHKTW_005307"
     CDS             complement(652..990)
                     /locus_tag="ACHKTW_005307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000856784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFO6466139.1"
                     /translation="MKTPRMTGKVIILRYLETHARFSAPELVEKLGVKRTIINQAAGK
                     MVREGLLVVDGYTDKITHYRKPTEKERLELAQRAEWQQEPSVIEACKRSEIMKRILFI
                     YGAGEELPVI"
     gene            complement(987..1196)
                     /locus_tag="ACHKTW_005308"
     CDS             complement(987..1196)
                     /locus_tag="ACHKTW_005308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6466140.1"
                     /translation="MNMTRNSKPKEHTMNDIRRISDKELTKMIESAQQLLSRRNPVVE
                     ETWWENLLAALTELRERRNNEGAAK"
BASE COUNT          205 a          305 c          302 g          400 t
ORIGIN      
        1 gttacgctga tatattttct tccgggtgaa gattcattct attccgctcc ctatgagtac
       61 agtcgcggta gctcaaaaag ttgttcaggg gcatttgttg atgatcctga tttgcaaaaa
      121 acaatattta tttgctaccc atacggtgac tatcaggatg gtaatgtgat ttatgtgaag
      181 aagcgtgtga atgcgctggg tgcggtggtc acctatgcat acgcgacaag cggccgattt
      241 cggtttgact gaaaaaagaa atcctgtgtt accctcgctg ctgagggtaa catgcgtgaa
      301 gtcatcgccc ggttcttgag ctgataatgc tttgcgccag caactgccag ccctctttct
      361 gttgcccgta cttgtcctga tacctgttta cctgcaagtt tccgtgcacc agaatcagat
      421 cgccttttct gtgttttgcc agttcttcgt gctgcttccc gaatgcaata aggtaaagcc
      481 acattgtggt tggctgttct gcgccatggc agggtagtga taccgccagt tgtgccgttg
      541 tgacgttggt tcctttggtt gttctccttg tgttcgggtc tgctgccagt cgtccctctg
      601 ttgcaacctg tgttggcata tttgtctccc gtgttggttt tattgttggg gttagatgac
      661 tggcagctct tcgccagcgc cgtagataaa tagaattcgc ttcatgattt cgctgcgctt
      721 gcaggcttca attacggacg gctcttgttg ccattctgcc cgttgcgcca gctccagtcg
      781 ttccttttcc gttggcttcc ggtaatgcgt tatcttgtcc gtgtacccgt ccacaaccag
      841 cagcccttcg cgtaccatct tcccggcggc ctgattaatg atggtccttt tcacgcccag
      901 tttttcaacc agctctggtg cgctgaagcg tgcgtgcgtt tccaggtagc gtaaaatgat
      961 tacctttccg gtcattctgg gtgtcttcat ttggcggctc cttcgttgtt ccggcgttcg
     1021 cggagttcgg tcagtgctgc cagtaaattt tcccaccatg tttcttccac aaccggattt
     1081 cgtcgtgaaa gcagctgctg cgccgactca atcattttcg taagctcttt gtcgctgatc
     1141 ctcctgatgt cgttcatggt gtgctccttc ggtttgctgt ttctggtcat gttcatggtc
     1201 ttttgttacc tg
//
LOCUS       DAZFQD010000329         1204 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.336, whole genome shotgun sequence.
ACCESSION   DAZFQD010000329 DAZFQD010000000
VERSION     DAZFQD010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1204
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.336"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            357..971
                     /gene="evgA"
                     /locus_tag="ACHKTW_005309"
     CDS             357..971
                     /gene="evgA"
                     /locus_tag="ACHKTW_005309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HFO6466141.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            976..>1204
                     /locus_tag="ACHKTW_005310"
     CDS             976..>1204
                     /locus_tag="ACHKTW_005310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFO6466142.1"
                     /translation="MKFLPYFFLLCCGLWSTISFADEDYIEYRGISSNNRVTLDPVRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQ"
BASE COUNT          380 a          228 c          246 g          350 t
ORIGIN      
        1 ttcatatcaa acacagtgca tgtgttgcac attatcccag gaacgttgga ggcctaattt
       61 aaatgaggtg caaaaaaaac aacagaggac taaaccgtgg cttttgcaat acaattctta
      121 cgcctgtagg attagtaaga agacttatag tgccaacttg aaactataaa tcatcggtac
      181 aatccctgat tttattgttg acatttcatt tatgccgact atttatatgg tatacttgtc
      241 gaattatctt aaaggaagct cagattttct tatttttatt gagaaaatga gatgacgcct
      301 tatgtctgta ttactacagg gagaagggag atgcttcatt gcaaagggaa taatctatga
      361 acgcaataat tattgatgac catcctcttg ctatcgcagc aattcgtaat ttattgatca
      421 aaaacgatat tgaaatctta gcggagttga ctgaaggggg aagcgccgtt cagcgggtgg
      481 aaacacttaa gcctgatatc gtcatcattg atgtcgatat ccccggagtt aacggtatcc
      541 aggtgttaga aacgctgagg aagcgccaat atagcggaat tattattatt gtctccgcta
      601 aaaatgacca tttttacggg aaacattgtg ctgatgctgg cgctaatgga ttcgtgagta
      661 aaaaagaagg catgaacaat atcattgcgg ctattgaagc tgcaaaaaat ggctactgct
      721 atttcccctt ctctctcaac cggtttgttg gaagtttaac gtccgaccag caaaaactcg
      781 actccttatc gaaacaagaa attagtgtca tgcggtatat tcttgatggc aaggataata
      841 atgacattgc tgaaaaaatg ttcatcagca acaaaactgt cagcacttat aaaagtcgcc
      901 tgatggaaaa attagaatgt aaatcactga tggatcttta cacattcgcg caacgtaaca
      961 aaatcggcta atcacatgaa gtttttaccc tatttttttc ttctctgttg tggtctttgg
     1021 tcgaccataa gtttcgcaga cgaagattac atcgaatatc gtggcatcag tagtaacaac
     1081 cgtgtcacac ttgatccagt acgtctgagc aacaaggaat tacgttggtt agcgagcaaa
     1141 aaaaatcttg tgattgcagt acacaagtcc caaacggcta ccttgttgca taccgattcg
     1201 cagc
//
LOCUS       DAZFQD010000330         1188 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.337, whole genome shotgun sequence.
ACCESSION   DAZFQD010000330 DAZFQD010000000
VERSION     DAZFQD010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1188
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.337"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..299)
                     /locus_tag="ACHKTW_005311"
     CDS             complement(<1..299)
                     /locus_tag="ACHKTW_005311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6466143.1"
                     /translation="MFPVSSIGNDISSDLVRRKMNDLPESPIVNNLEALAPGIEKLKQ
                     TSIQMVTLLNALQPGGKCIITGDFQKELAYLQNVIIYNDSSLRMDFFGYNALIIQR"
     gene            complement(321..860)
                     /gene="yhaB"
                     /locus_tag="ACHKTW_005312"
     CDS             complement(321..860)
                     /gene="yhaB"
                     /locus_tag="ACHKTW_005312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhaB"
                     /protein_id="HFO6466144.1"
                     /translation="MKGFPIAHIFHPSIPPMHAVVNNHNRNIDYWTVKRKFAEIVSTN
                     DVNKIYSISNELRRVLSAITALNFYQGDVPSVMIRIQPENMSPFIIDISTGEHDDYII
                     QTLYVGTFAPFGEQCTCSAVNKKELECIKETISKYCAKFTRKEAILTPPAHFNKTSIT
                     SDCWQILFFSPDHFNNDFY"
     gene            complement(1116..>1188)
                     /locus_tag="ACHKTW_005313"
     CDS             complement(1116..>1188)
                     /locus_tag="ACHKTW_005313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TdcR"
                     /protein_id="HFO6466145.1"
                     /translation="EQLINVNFFSSDRTSFCECNRFP"
BASE COUNT          368 a          208 c          224 g          388 t
ORIGIN      
        1 cgttgaataa ttagggcgtt ataaccaaaa aaatccatac gaagagacga atcattataa
       61 ataattacat tttgtaagta cgctaattct ttttgaaaat caccagtgat aatgcatttt
      121 ccacctggtt gtaacgcgtt aagtaaagtg accatttgaa tagaggtctg ttttagtttt
      181 tctatacctg gtgctagtgc ttcgaggtta ttaactatgg ggctttcagg aagatcattc
      241 atttttcgac ggactaaatc actactaata tcattaccaa ttgatgaaac agggaacatt
      301 attttatcct ttataaataa tcaataaaaa tcattattaa aatgatcggg tgaaaaaaag
      361 agaatttgcc aacaatctga agtaatgctg gttttattaa agtgcgcagg aggtgttaag
      421 atggcttctt ttcgggtgaa ttttgcacaa tacttagaaa tcgtctcctt aatacattcc
      481 agctcttttt tattgacggc tgagcaagta cattgttcac caaaaggtgc aaaagtgcct
      541 acatataatg tttgtatgat ataatcgtca tgttctcctg tagaaatatc tatgatgaat
      601 ggactcatat tttccggttg gattcggatc atgacagaag gaacatcgcc ttgatagaaa
      661 ttcaatgcag taattgcaga taatactctc cgcagttcat tacttatact gtaaatttta
      721 ttaacgtcat tggtggagac aatttctgca aactttcttt ttaccgtcca ataatcaata
      781 tttctattgt gattgttaac cactgcgtgc attggcggga ttgaagggtg aaaaatatgt
      841 gcaattggga agcctttcat tgatgcctgc cttgtcgtca tgacaaacaa taatagatgc
      901 ttattttcct cgcagggttc tttcgcacta tcaaaaaacg ctcatttacc cggcgttaca
      961 gtaaacgaag caacattctg tggcctacga cgatcaaaag ctctgtgaaa atttcccttg
     1021 ttgtctttgt ctgaccttat cgcggcgatc agcaattatt cgttttgtga tcctcaacac
     1081 aaaatgctat tcgtcgccta tgaggggaga agaatttatg gaaaacgatt gcattcacaa
     1141 aatgacgtcc tgtcagaaga gaaaaagttg acgttaatca attgttcg
//
LOCUS       DAZFQD010000331         1184 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.338, whole genome shotgun sequence.
ACCESSION   DAZFQD010000331 DAZFQD010000000
VERSION     DAZFQD010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1184
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.338"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(65..1009)
                     /gene="leuO"
                     /locus_tag="ACHKTW_005314"
     CDS             complement(65..1009)
                     /gene="leuO"
                     /locus_tag="ACHKTW_005314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HFO6466146.1"
                     /translation="MPEVQTDHSETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
BASE COUNT          352 a          269 c          245 g          318 t
ORIGIN      
        1 ttctacattc aataaaaaat gcagaataaa ccagacattc atgtctggcc tattctgcat
       61 tcagttagcg tttgcaaatt gagactaatt gttcttccat ccactgatgg cctttatcgc
      121 gcccggcagc ttcatgccag gagagataac aggttctgct gttttgtttt aacggcagcg
      181 gtaatacctg taattctaag gattcagcga actcttcagc cagccaacgc ggcgcaatag
      241 cgaccaaatg cgtttgcgac accacgctaa gtacgctcat cattgccatg ccctgatacg
      301 cgatactggc ttgcttatct accgtgtcat accaaggttg actaaatgac gcgaaacgat
      361 cgagcgaaac cgccgcatgt tgttcgttat aaacatcatg tttcagtaac gggcctttaa
      421 ttgttggatg atttttgctg gctaccagca ccatttcatc tttaaataat ggcacgctgg
      481 taaattcagg acgatggaag tcttcataac taatcacaaa ctccgtttcc tgataacgca
      541 gctgatgttc agtgttctga tttaatgaag acttgaacat aacatgtata tttggcgcaa
      601 tctgctcaat gtgattataa atctgcgagg tcagaatgct gtctaacggg ctgcaaacac
      661 aaagatgaaa tacacgttca ctgctcgcgg gttcaaaacc tgaaccaggc aattcatttt
      721 gtactagttg caatgcctga cgaactgagc caaaaagttg aaatgcgcga gcagtcggtt
      781 gaataccacg gccataacga acaaaaagct cgtcattaaa catcaccttc aggcgtgcaa
      841 cagcgttact gaccgcaggt tgcgacatac ccagaacatg agcggcacgg gtaatgtttt
      901 gctcctgcat cacggcatcg aaaacggtta ataagttgag atcgaccatg cgtagctgtg
      961 gcttgcttaa ctccgccgtc tctgaatgat ctgtttgtac ctctggcata cttaactcca
     1021 ctgtcacact taactccctt tcccttattg gaatgcagag aaataatcct gaagaatatg
     1081 atttatcatg catataaaat aagaaaaagc aaaatgagta aaattcggaa aacataaaga
     1141 tacgggttct gataaaaaca gaatccattg ccgtggaatg agtc
//
LOCUS       DAZFQD010000332         1120 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.339, whole genome shotgun sequence.
ACCESSION   DAZFQD010000332 DAZFQD010000000
VERSION     DAZFQD010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1120
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.339"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            452..1003
                     /gene="ybcL"
                     /locus_tag="ACHKTW_005315"
     CDS             452..1003
                     /gene="ybcL"
                     /locus_tag="ACHKTW_005315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Raf kinase inhibitor-like protein YbcL"
                     /protein_id="HFO6466147.1"
                     /translation="MKTLIVSTVLAFITFSAQAAAFQVTSNEIKTGEQLTTSHVFSGF
                     GCEGGNTSPSLTWSGVPEGTKSFAVTVYDPDAPTGSGWWHWTVVNIPATITYLPVDAG
                     RRDGTKLPTGAVQGRNDFGYAGFGGACPPKGDKPHHYQFKVWALKTEKIPVDSNSSGA
                     LVGYMLNANKIATAEITPVYEIK"
     gene            1013..>1120
                     /locus_tag="ACHKTW_005316"
     CDS             1013..>1120
                     /locus_tag="ACHKTW_005316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFO6466148.1"
                     /translation="MLARGKTNLKIEEIRMHKHHEIHRVKPLMPALCRIR"
BASE COUNT          352 a          176 c          250 g          342 t
ORIGIN      
        1 attttaagtt atgctatggt gataaagtgc aagacagaat taattatctt tgacgaaact
       61 taatgggtaa ttactttgtt tgctcccaca agcgagtttt gtacggctgt attggggtag
      121 taaatgagct atacaatctt aatcatttgt taggtgagaa ctcttggtcg cagattcaaa
      181 tactgaaaat acgtgacaaa ttattatgag caaaatggtg tatgtcacgt attttgaatg
      241 ataggttaaa aaataacacc gactttcgta ggtattacta ataataaagc agagttttta
      301 gatagtatca atgtgctttg tgtatattgt ggcaaataat tgggttgggg gtacaattgt
      361 gattgctttt gcataaaaat tgcgccttta tgcataatga gataaaggaa tatcaaataa
      421 aataacgata ggtcataaca aagaggtttt tatgaaaaca cttatcgttt caactgtatt
      481 ggcattcata acattttctg cgcaggctgc agcatttcag gtcactagta atgaaataaa
      541 aacaggagag caacttacaa cgtctcatgt cttttctgga tttgggtgtg aaggtggtaa
      601 tacatcgccc tcattaacct ggtctggtgt tcctgaaggt accaaaagct ttgccgtaac
      661 tgtatatgat ccagatgcac ctacaggcag tggttggtgg cattggactg ttgttaatat
      721 tccagcaaca ataacatatt tgcccgttga tgcagggaga cgtgatggaa caaaactgcc
      781 gactggtgct gttcaaggcc gaaatgattt tggctatgct gggtttggtg gcgcatgtcc
      841 tcctaaagga gataaaccac atcattacca gtttaaagta tgggctctaa aaactgaaaa
      901 gattcctgta gattctaact ccagcggagc gttagttggt tatatgctta atgctaataa
      961 aatcgcaacc gctgaaataa caccagttta tgagataaag tagggtgaga gtatgctggc
     1021 aagaggtaag actaacttaa agatcgaaga aatacggatg cataaacatc atgagattca
     1081 tagggttaag cctcttatgc cagctttgtg tcgtatccgt
//
LOCUS       DAZFQD010000333         1109 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.340, whole genome shotgun sequence.
ACCESSION   DAZFQD010000333 DAZFQD010000000
VERSION     DAZFQD010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1109
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.340"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>1109
                     /gene="rhsC"
                     /locus_tag="ACHKTW_005317"
     CDS             <1..>1109
                     /gene="rhsC"
                     /locus_tag="ACHKTW_005317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhs element protein RhsC"
                     /protein_id="HFO6466149.1"
                     /translation="ARQGDMTQYGGSIVQGSAGVRIGAPTGVACSVCPGGVTSGHPVN
                     PLLGAKVLPGETDIALPGPLPFILSRTYSSYRTKTPAPVGSLGPGWKMPADIRLQLRD
                     NTLILSDNGGRSLYFEHLFPGEDGYSRSESLWLVRGGVAKLDEGHRLAALWQALPEEL
                     RLSPHRYLATNSPQGPWWLLGWCERVPEADEVLPAPLPPYRVLTGLVDRFGRTQTFHR
                     EAAGEFSGEITGVTDGAGRHFRLVLTTQAQRAEEARQQAISGGTEPSAFPDTLPGYTE
                     YGRDNGIRLSAVWLTHDPEYPENLPAAPLVRYGWTPRGELAAVYDRSNTQVRSFTYDD
                     KYRGRMVAHRHTGRPEICYRYDSDGRVTEQLNPAG"
BASE COUNT          200 a          345 c          373 g          191 t
ORIGIN      
        1 gcgcgtcagg gcgacatgac gcagtatggc ggtagcattg tccagggttc agccggggtg
       61 cgcattggtg cccccaccgg cgtggcctgt tcggtgtgcc ccggcggggt gacgtccggc
      121 catccggtca atccgctgct gggtgcaaag gtccttcccg gtgaaaccga catcgccctg
      181 cccggcccgc tgccgttcat tctctcccgc acctacagca gttaccggac aaaaacgccc
      241 gcgccggtgg ggagcctcgg ccccggctgg aaaatgcctg cggatatccg cttacagctg
      301 cgcgataaca cactgatact cagtgataac ggcggcagaa gcctgtattt tgagcacctg
      361 tttcccggtg aggacggtta cagccgcagc gaatcactct ggctggtgcg cggcggcgtg
      421 gcgaaactgg atgaaggtca ccggctggcc gcactctggc aggcgctgcc ggaagaactc
      481 cgcttaagtc cgcatcgtta tctggcgaca aacagtccgc aggggccgtg gtggctgctc
      541 ggctggtgtg agcgggtgcc ggaagcggat gaggtgctgc ctgcgccgct gccgccgtac
      601 cgggtactga ccgggctggt ggaccgcttc gggcgcacac agacgttcca ccgcgaagcc
      661 gccggtgaat tcagcggcga aatcaccggc gtgacggatg gtgccgggcg tcacttccgg
      721 ctggtactga ccacgcaggc gcagcgggca gaagaagccc ggcagcaggc catttccggc
      781 gggacggaac cgtccgcttt tcctgatacc ctgccgggtt acaccgaata tggccgggac
      841 aacggcatcc gtctgtctgc cgtgtggctg acgcacgacc cggaataccc ggagaattta
      901 cctgccgcgc cgctggtgcg ctatggctgg acgccacgcg gcgaactggc ggcggtgtat
      961 gaccgcagca atacacaggt gcgcagcttt acttacgatg ataaataccg gggccggatg
     1021 gtggcgcacc gtcacacggg ccgaccggaa atctgttacc gttacgacag cgacgggcgg
     1081 gtgacagaac agctaaaccc ggcaggctt
//
LOCUS       DAZFQD010000334         1097 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.341, whole genome shotgun sequence.
ACCESSION   DAZFQD010000334 DAZFQD010000000
VERSION     DAZFQD010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1097
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.341"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..872
                     /locus_tag="ACHKTW_005318"
     CDS             <1..872
                     /locus_tag="ACHKTW_005318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HFO6466150.1"
                     /translation="LYSVRHLVFNGFELISQQFSRERQPHPSVAPQWVTRTNYAVSDL
                     TGRPLMLFNSEGKTVWRPGQTSLWGLALSLPADTGYPDPRGELDPEADPGLLYAGQWQ
                     DAESGLCYNRFRYYEPESGMYLVSDPLGLQGGEQTYRYVPNPLGYVDPLGLAVCPKLY
                     EIYKGLRAQGFNPAEARDLAKWFNSSWGKADVYLRSAIGSGKIDYVGISRNVLNRYPQ
                     GKYDLELLTRLTGQLPRNQARAIEQAIINAKRGNGFTNIQNSIDPAKDHFDAAVKWGE
                     EWLKSNGFGGWLQ"
     gene            860..>1097
                     /locus_tag="ACHKTW_005319"
     CDS             860..>1097
                     /locus_tag="ACHKTW_005319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6466151.1"
                     /translation="MAAIKMGVLMNNQLSEICSDSIVSMVKKGVRRFKRPEVGDLFTL
                     EMQDIGFIHGMVARNDLEFVKGQADFNIIYIYKEI"
BASE COUNT          319 a          205 c          313 g          260 t
ORIGIN      
        1 aactgtacag cgtacggcac ctggtgttta acggctttga gctgataagc cagcagttca
       61 gccgggaacg gcagccgcat ccgtcggtgg ccccgcagtg ggtgacgcga acgaattatg
      121 cggtgagcga cctgacgggc cgtccgctga tgctctttaa cagtgaaggt aaaaccgtct
      181 ggcgaccggg gcagaccagc ctgtgggggc tggcactcag tctgcccgca gacaccggct
      241 acccggaccc gcgcggggaa ctggacccgg aagccgaccc cggcctgctg tatgcaggac
      301 agtggcagga tgcggaatcc gggctgtgct ataaccggtt ccggtattac gagccggaga
      361 gcgggatgta cctggtgagt gatccgctgg ggttgcaggg gggtgagcag acttaccggt
      421 atgtgcctaa tccgctgggg tatgttgatc cgttagggtt ggctgtatgt cctaaattgt
      481 atgaaatata caagggatta agagcacaag gatttaaccc agcagaagct agagatctag
      541 caaaatggtt caatagtagc tggggaaagg ctgatgtata tcttcgttca gcaataggat
      601 caggaaaaat agattatgtt ggaataagta gaaatgtgct taatcgttat ccgcaaggta
      661 aatatgattt ggagttactc acaagactaa caggacaact tccgaggaat caagcaagag
      721 caatagaaca agctatcatt aatgctaaaa gaggaaacgg cttcacaaat atccagaata
      781 gtatagatcc tgctaaagat cattttgatg cagcggtaaa atggggtgag gagtggttaa
      841 agagtaatgg atttggtgga tggctgcaat aaaaatgggg gtattaatga ataaccaatt
      901 aagcgaaata tgttcagact ccattgtttc aatggttaaa aaaggagtgc gaagattcaa
      961 gagacctgaa gtgggcgatc tttttacact agaaatgcaa gatataggtt ttattcatgg
     1021 catggttgct cgaaatgacc ttgagtttgt aaagggacaa gctgatttta atattattta
     1081 tatttacaag gaaataa
//
LOCUS       DAZFQD010000335         1097 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.342, whole genome shotgun sequence.
ACCESSION   DAZFQD010000335 DAZFQD010000000
VERSION     DAZFQD010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1097
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.342"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..591
                     /locus_tag="ACHKTW_005320"
     CDS             <1..591
                     /locus_tag="ACHKTW_005320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO6466152.1"
                     /translation="YPEAGLYLAEMLYLPIDLIESFQKLYTVTDQIRNKTSFFLPQNP
                     ELIYCWEQLKTSVSRGFSTKIQEHLAMGVLLSLGVNHVNHLLLSYSKQSLISRCYNLL
                     LSEPGTKWTANKVARYLYISVSTLHRRLASEGVSFQSILDDVRLNNALSAIQTTVKPI
                     SEIARENGYKCPSRFTERFHNRFNITPREIRKASRE"
     gene            708..809
                     /locus_tag="ACHKTW_005321"
     CDS             708..809
                     /locus_tag="ACHKTW_005321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6466153.1"
                     /translation="MRTYNPNSLLPSQMQKCTCNSLHLAFDLCGGEA"
     gene            806..>1097
                     /gene="ybcN"
                     /locus_tag="ACHKTW_005322"
     CDS             806..>1097
                     /gene="ybcN"
                     /locus_tag="ACHKTW_005322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA base-flipping protein YbcN"
                     /protein_id="HFO6466154.1"
                     /translation="MNLSQDGIKLHRGNFTAIGRQIQPYLEEGKCFRMVLKPWREKRS
                     LSQNALSHMWYSEISEYLISRGKTFATPAWVKDALKHTYLGYETKDLVDVVTG"
BASE COUNT          332 a          236 c          225 g          304 t
ORIGIN      
        1 tatcctgaag cagggcttta tcttgcagaa atgctttact tacccattga tttaattgag
       61 agtttccaaa aactttatac ggtaactgat caaatacgta acaaaacaag tttcttttta
      121 cctcagaatc ctgagttaat atattgttgg gagcaactaa aaacatctgt ttcccgaggc
      181 ttctcaacta aaattcagga gcacttagca atgggcgttc tactttcgtt aggagtgaat
      241 catgttaatc atttactttt atcatatagt aaacaatcat tgataagtcg ttgttataac
      301 ctgctgctat ccgaacccgg cacaaaatgg acagcaaaca aggttgctcg atatctctac
      361 atttctgttt ctacattaca tcgccgtcta gcaagcgagg gggtaagttt ccaaagtata
      421 ctggacgatg tgaggttaaa taatgcgttg tctgctatac aaacgacggt aaaacctata
      481 agcgagattg ccagagaaaa tggttataag tgtccttctc gttttactga aagatttcat
      541 aatcgtttta atataacacc aagagagata agaaaagctt ccagagagta aaagtgtttt
      601 aagaaggagc aattctatcg attttgattt tgggaaatca acacggcata attatgtcac
      661 cggagcctga acaactccgg tgacttctgc gctaaacggg gacgtttatg cgcacataca
      721 atccaaactc tcttctccct tcacagatgc agaaatgcac ctgcaattct ttgcatctag
      781 cgtttgacct ctgcggaggt gaagcgtgaa cctctcacaa gacggcatca aattacatcg
      841 cggcaacttc accgctatcg gtcggcagat ccagccttat ctggaggagg gcaaatgctt
      901 tcgcatggtg cttaaaccgt ggcgtgagaa acgcagtctt tcccagaatg cactcagcca
      961 catgtggtac agcgaaatca gtgaatacct catcagcagg ggtaaaacgt tcgccactcc
     1021 agcttgggta aaagatgctc tcaaacacac atatctcggt tatgaaacca aagacctggt
     1081 tgatgtcgta accggtg
//
LOCUS       DAZFQD010000336         1069 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.343, whole genome shotgun sequence.
ACCESSION   DAZFQD010000336 DAZFQD010000000
VERSION     DAZFQD010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1069
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.343"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            71..>1069
                     /gene="phoE"
                     /locus_tag="ACHKTW_005323"
     CDS             71..>1069
                     /gene="phoE"
                     /locus_tag="ACHKTW_005323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HFO6466155.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNDSKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDN"
BASE COUNT          320 a          224 c          264 g          261 t
ORIGIN      
        1 aaaaactttc ctgttttcaa cggggctctc ccgctgaata ttcgcgcgtt aattaaaatc
       61 aggaatgaaa atgaaaaaga gcactctggc attagtggtg atgggcattg tggcatctgc
      121 atccgtacag gccgcagaaa tatataacaa agacggtaat aaactggatg tctatggcaa
      181 agttaaagcc atgcattata tgagtgataa cgacagtaaa gatggcgacc agagttatat
      241 ccgttttggt tttaaaggcg aaacacaaat taacgatcaa ctgactggct atggccgttg
      301 ggaagcggag tttgccggaa ataaagcgga gagtgatact gcacagcaaa aaacgcgtct
      361 cgcttttgcc ggattgaagt ataaagattt gggttctttc gactatggcc gtaacctggg
      421 cgcgttgtat gacgtggaag cctggaccga tatgttcccg gaatttggtg gcgactcctc
      481 ggcgcagacc gacaacttta tgaccaaacg cgccagcggt ctggcgacgt atcggaacac
      541 cgacttcttc ggcgttatcg atggcctgaa cttaaccctg caatatcaag ggaaaaacga
      601 aaaccgcgac gttaaaaagc aaaacggcga tggcttcggc acgtcattga catatgactt
      661 tggcggcagc gatttcgcca ttagtggtgc ctataccaac tcagatcgca ccaacgagca
      721 gaacctgcaa agccgtggca ctggcaagcg tgcagaagca tgggcaacag gtctgaaata
      781 cgatgccaat aatatttatc tggcaacttt ttattctgaa acacgcaaaa tgacgccaat
      841 aactggcggc tttgccaata agacacagaa ctttgaagcg gtcgctcaat accagtttga
      901 ctttggtctg cgtccatcgc tgggttatgt cttatcgaaa gggaaagata ttgaaggtat
      961 cggtgatgaa gatctggtca attatatcga tgtcggggct acatattatt tcaacaaaaa
     1021 tatgtcagcg tttgttgatt ataaaatcaa ccaactggat agcgataac
//
LOCUS       DAZFQD010000337         1064 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.344, whole genome shotgun sequence.
ACCESSION   DAZFQD010000337 DAZFQD010000000
VERSION     DAZFQD010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1064
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.344"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..759
                     /locus_tag="ACHKTW_005324"
     CDS             <1..759
                     /locus_tag="ACHKTW_005324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HFO6466156.1"
                     /translation="GNYEFPQPLHDALDKFQADTGIDIDMHIDAASGGFLAPFVAPEI
                     VWDFRLPRVKSISASGHKFGLAPLGCGWVIWRDEEALPQELVFNVDYLGGQIGTFAIN
                     FSRPAGQVIAQYYEFLRLGREGYTKVQNASYQVAAYLADEIAKLGPYEFICTGRPDEG
                     IPAVCFKLKDGEDPGYTLYDLSERLRLRGWQVPAFTLGGEATDIVVMRIMCRRGFEMD
                     FAELLLEDYKASLKYLSDHPKLQGIAQQNSFKHT"
BASE COUNT          252 a          271 c          255 g          286 t
ORIGIN      
        1 ggtaactatg agttcccaca accgctgcac gatgcgctgg ataaattcca ggccgacacc
       61 ggtatcgaca tcgacatgca catcgacgct gccagcggtg gcttcctggc accgttcgtc
      121 gccccggaaa tcgtctggga cttccgcctg ccgcgtgtga aatcgatcag tgcttcaggc
      181 cataaattcg gtctggctcc gctgggctgc ggctgggtta tctggcgtga cgaagaagcg
      241 ctgccgcagg aactggtgtt caacgttgac tacctgggtg gtcaaattgg tacttttgcc
      301 atcaacttct cccgcccggc gggtcaggta attgcacagt actatgaatt cctgcgcctc
      361 ggtcgtgaag gctataccaa agtacagaac gcctcttacc aggttgccgc ttatctggcg
      421 gatgaaatcg ccaaactggg gccgtatgag ttcatctgta cgggtcgccc ggacgaaggc
      481 atcccggcgg tttgcttcaa actgaaagat ggtgaagatc cgggatacac cctgtacgac
      541 ctctctgaac gtctgcgtct gcgcggctgg caggttccgg ccttcactct cggcggtgaa
      601 gccaccgaca tcgtggtgat gcgcattatg tgtcgtcgcg gcttcgaaat ggactttgct
      661 gaactgttgc tggaagacta caaagcctcc ctgaaatatc tcagcgatca cccgaaactg
      721 cagggtattg cccagcagaa cagctttaaa cacacctgat aacataacgt tgtaaaaacc
      781 gaatgcccag cctttaaaaa aacagctggg cattcggttg cttattaatc gcaataatat
      841 attggctgtc tattcatcgt gttgataaga tatatgttat atgaatgttt attaaatgta
      901 gcgattgcct ttaatgtcac gaaaagggat tatttgcttg ctattaattt cactgcgttc
      961 ttaaacacgt ttttgtatta taaatccagt catcctgccc gtagaacatc acacattatc
     1021 atcctgttct cccgcttcgt ttaaatttat ttatcaatca attt
//
LOCUS       DAZFQD010000338         1057 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.345, whole genome shotgun sequence.
ACCESSION   DAZFQD010000338 DAZFQD010000000
VERSION     DAZFQD010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1057
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.345"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>1057
                     /locus_tag="ACHKTW_005325"
     CDS             <1..>1057
                     /locus_tag="ACHKTW_005325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000717715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HFO6466157.1"
                     /translation="KIDFLNLKMMNQPYRADILNAITKVVDSGWYIQGEEVRNFEHEF
                     ASFIGSKYCVGVANGLDALSITLKAWILLGKIKEGDEVIVPANTFIATVLAITQNHLK
                     PIFVEPDEETFNINVDMIRNVITEKTKVIIPVHLYGQVAEMNKIRELADLHDLLILED
                     SAQAHGAMYNNLKAGNLGDAGAFSFYPGKNLGALGDAGAVVTDNQELAETIRAIGNYG
                     AHVKYKHDVQGVNSRLDEIQAAILRVKLKYINNEIESRRIIANNYLTKIINPIIRLPK
                     IDNNAELSHVWHLFVIKTNERNKLQDYLAKHSVQTLIHYPIPIHKQLAYNNLNTYSYP
                     ITEKLHDEILSLPIGSFL"
BASE COUNT          390 a          154 c          200 g          313 t
ORIGIN      
        1 aaaatagact tcctaaatct aaaaatgatg aatcaacctt atcgagcgga tatactaaat
       61 gcaataacta aagttgttga ttcggggtgg tatattcaag gtgaagaagt cagaaatttc
      121 gaacatgagt ttgcatcatt tattggttcg aagtattgtg tcggtgttgc caatggctta
      181 gatgcattga gcattacctt aaaagcatgg atattattag gcaaaattaa agaaggggat
      241 gaggtaatag ttccagcaaa tacatttatt gcaactgtat tagctatcac ccaaaatcat
      301 cttaaaccaa tatttgtaga accagatgaa gaaacattca acattaatgt tgatatgatt
      361 agaaatgtca tcacggagaa gactaaagtt attatacctg ttcatcttta tgggcaggtg
      421 gcagaaatga ataaaattcg tgaattagct gaccttcatg atctgttgat tttggaggac
      481 tctgcgcaag cacatggagc catgtataat aaccttaagg caggaaacct tggtgatgca
      541 ggcgctttta gtttctaccc aggtaaaaac ttgggggcat taggggatgc tggtgctgta
      601 gtgactgaca atcaagagct ggcagaaact ataagagcaa ttggtaatta tggtgcacat
      661 gtaaaatata aacatgatgt acaaggggtt aatagccgtc tagatgagat tcaagcagcg
      721 attctacggg ttaaattaaa atatataaac aatgaaatag aatctagaag aataatagca
      781 aataactatt taacaaaaat aatcaatcca attatcagat tgcctaaaat agataataat
      841 gcagaattat cacatgtatg gcatctcttt gttataaaaa caaatgaaag gaataaacta
      901 caagattatt tagctaaaca tagtgttcaa acattgatcc attatccaat tcctatacat
      961 aagcagttag cgtataataa tctgaataca tattcttatc cgataactga gaaattacat
     1021 gatgaaatat tatcacttcc aattggtagc tttttat
//
LOCUS       DAZFQD010000339         1052 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.346, whole genome shotgun sequence.
ACCESSION   DAZFQD010000339 DAZFQD010000000
VERSION     DAZFQD010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1052
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.346"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(137..424)
                     /locus_tag="ACHKTW_005326"
     CDS             complement(137..424)
                     /locus_tag="ACHKTW_005326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000865085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO6466158.1"
                     /translation="MKVKGVPFSQVKQKLLNTPEAVKGYEDADKELEMVEMLYEMREK
                     AGLTKSALAERMGLRPSAISRLESNPLGASMKTLTKYAQACGATINIHAVY"
     gene            complement(424..735)
                     /locus_tag="ACHKTW_005327"
     CDS             complement(424..735)
                     /locus_tag="ACHKTW_005327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000483535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO6466159.1"
                     /translation="MFNVITHPAALNELIELPDNLRGRMARLIERLEQEGNKMKMPHS
                     RVIGGGLFELRVGDKNIARTLYAYATGNEIYLLHAFVKKTQKTPTKAIEIARMRLKEM
                     K"
     gene            complement(841..981)
                     /locus_tag="ACHKTW_005328"
     CDS             complement(841..981)
                     /locus_tag="ACHKTW_005328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6466160.1"
                     /translation="MAENGYGLAGLEMGKVKSINQYRLTPGFGGFTPVPYATTECRLP
                     CR"
BASE COUNT          267 a          254 c          203 g          328 t
ORIGIN      
        1 tgtccttgac ccacgatatt cagactgtcg ttgtatctta agttgacgtt acccgctgac
       61 ttttatgtgt tgaaaaacat ccattttaga tgtttttcaa tcttttgatt agaaatacat
      121 atcgttaaca tgcaaatcaa tagacagcat gaatattgat ggttgcacca caggcctgag
      181 catattttgt taaagttttc atactggctc ctaaaggatt actttccaag cgactgatgg
      241 ctgacgggcg taaccccatt cgttcagcca gagcagactt tgttaacccc gctttttctc
      301 tcatttcgta caacatttct accatctcca gttccttatc ggcatcttca taccctttga
      361 cagcttctgg agtattaagc agtttttgct ttacctgact gaacggaaca ccttttactt
      421 tcattatttc atctccttca ggcgcatcct agcgatttca atagctttcg ttggtgtctt
      481 ttgcgtcttt ttcacaaatg catgcagcag atagatttcg tttccagttg cataagcgta
      541 cagtgtcctt gcgatatttt tatccccaac tctcagttca aaaagtcctc cgccaattac
      601 acgactatga ggcattttca ttttgttacc ttcttgttcc agtcgttcaa tcagcctagc
      661 catgcgacca cgcagattat ctggtaattc aatcaactca ttcagtgcgg ccgggtgagt
      721 aattacatta aacatattat agcctccgaa aggataaaat acaccagaac aggaaaattg
      781 cgcaaatatc aaatttcact gataaatgaa atacatgtta agtacagttt tgatgccgca
      841 tcagcggcat ggaaggcggc attccgttgt tgcatatggc acaggagtaa aaccgccgaa
      901 gcccggcgtc agccggtact gattgataga tttcacctta cccatctcca gccctgctaa
      961 gccatacccg ttttcagcca tgatagagct tctgtgcgcg gtcggagtgg tcccgacgag
     1021 ggtttacccg aagtcggggc gtatctccgc gt
//
LOCUS       DAZFQD010000340         1032 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.347, whole genome shotgun sequence.
ACCESSION   DAZFQD010000340 DAZFQD010000000
VERSION     DAZFQD010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1032
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.347"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            328..924
                     /gene="fimE"
                     /locus_tag="ACHKTW_005329"
     CDS             328..924
                     /gene="fimE"
                     /locus_tag="ACHKTW_005329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418733.1"
                     /note="upgraded from regulatory protein to switch DNA
                     invertase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimE"
                     /protein_id="HFO6466161.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTQERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GHRNIRHTVRYTASNAARFAGLWERNNLINEKLKREEV"
BASE COUNT          303 a          189 c          211 g          329 t
ORIGIN      
        1 aatggtttat gttcttcaaa ataaataaac aaaatcattc ataaatttac acatcactta
       61 aattctcctg tttccgcact tttttcttta ttttttaagc aactggaagt taatccactg
      121 caatctattg ttatattgaa tcaaatcaat gaaaatagat gttatcacat cagtgatatt
      181 ttatttttgt atgatattta atgtaattga ctgatagcca catcacttcg tgtgtggtta
      241 tctttttatc tattgggcta attttgaccg attgaggttt cctataggta ttcattcaaa
      301 tatatctcag ttaggagtac tactattgtg agtaaacgtc gttatcttac cggtaaagaa
      361 gttcaggcca tgatgcaggc ggtttgttac ggggcaacgg gagccagaga ttattgtctt
      421 attctgttgg catatcggca tgggatgcgt attagtgaac tgcttgatct gcattatcag
      481 gatcttgacc ttaatgaagg tcgaataaat attcgccgac tgaagaacgg attttctacc
      541 gttcacccgt tacgttttga tgagcgtgaa gccgtggaac gctggaccca ggaacgtgct
      601 aactggaaag gcgctgaccg gaccgacgct atatttattt ctcgccgcgg gagtcggctt
      661 tctcgccagc aggcctatcg cattattcgc gatgccggta ttgaagctgg aaccgtaacg
      721 cagactcatc ctcatatgtt aaggcatgct tgtggttatg aactggcgga gcgtggtgcc
      781 gatacccgtt taattcagga ttatctcggg catcgaaata ttcgccatac tgtacgttat
      841 accgccagta atgctgctcg ttttgccgga ctatgggaaa gaaataatct cataaacgaa
      901 aaattaaaaa gagaagaggt ttgatttaac tcattgataa taaagttaaa aaacaaataa
      961 atacaagaca attggggcca aactgtccat atcataaata agttacgtat tttttctcaa
     1021 gcataaaaat at
//
LOCUS       DAZFQD010000341         1028 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.348, whole genome shotgun sequence.
ACCESSION   DAZFQD010000341 DAZFQD010000000
VERSION     DAZFQD010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1028
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.348"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..510
                     /locus_tag="ACHKTW_005330"
     CDS             <1..510
                     /locus_tag="ACHKTW_005330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6466162.1"
                     /translation="FYQYPLNPVQYIDSMGLASKYGHLNNGGYGARPNKPPTPDPSKL
                     PDIAKQLRLPYPIDQASSAPNVFKTFFRALSPYDYTLYCRKWVKPNLTCTPQDDSQYP
                     GMDTKTASDYLPQTNWPTTQLPPGYTCAEPYLFPDINKPDGPATAGIDDLGEILAKMK
                     QRTSRGIRK"
     gene            507..>1028
                     /gene="ybfC"
                     /locus_tag="ACHKTW_005331"
     CDS             507..>1028
                     /gene="ybfC"
                     /locus_tag="ACHKTW_005331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbfC"
                     /protein_id="HFO6466163.1"
                     /translation="MKRVLFFLLMIFVSFGVIADCEIQAKDHDCFTIFAKGTIFSAFP
                     VLNNKAMWRWYQNEDIGEYYWQTELGTCKNNKFTPSGARLLIRVGSLRLNENHAIKGT
                     LQELINTAEKTAFLGDRFRSYIRAGIYQKKSSDPVQLLAVLDNSIMVKYFKDEKPTYA
                     RMTAHLPNKNESYE"
BASE COUNT          346 a          195 c          218 g          269 t
ORIGIN      
        1 ttttatcagt atccgctgaa tccggttcag tatatagatt caatgggact ggcatcaaaa
       61 tatggacact taaataatgg cggatatgga gcgagaccca acaaaccgcc tacgcccgat
      121 ccaagtaaat tgccggacat agcgaaacaa ttaagactgc catatcctat tgaccaggcc
      181 agtagtgcgc ctaatgtttt caaaacattc ttcagagcat taagccctta cgactacaca
      241 ctgtattgca ggaagtgggt aaaaccaaat ctgacttgta cgccacagga tgattcccag
      301 tatccaggga tggatacaaa gacagcaagt gattacctgc cacagacaaa ttggccaaca
      361 actcaattac caccaggata tacttgtgca gaaccctatt tattcccaga cattaataaa
      421 cccgatgggc cagcaacagc agggatagat gatttgggtg aaattttagc taagatgaaa
      481 cagagaacat cgagaggaat aagaaaatga aaagagtttt gttctttttg ctgatgatat
      541 ttgttagttt tggtgttata gctgattgcg aaatacaagc taaagatcat gattgtttta
      601 ctattttcgc taagggtact attttttccg cgtttcctgt cttaaataat aaagccatgt
      661 ggagatggta tcagaatgag gacatcggtg agtattattg gcaaacagaa ctgggtacat
      721 gtaaaaataa taaattcaca ccaagcgggg cgagactctt aattcgcgtc gggtcactac
      781 gccttaatga aaatcatgcc atcaaaggaa cgttgcagga gttgataaat acagcagaaa
      841 aaacggcgtt tctcggcgat cggtttagaa gttatataag agcgggtatc tatcaaaaaa
      901 agagcagtga tccagttcag ttgttggcag tccttgataa ttcaatcatg gtaaaatatt
      961 ttaaagatga gaaaccaact tatgctcgaa tgacagcaca tctcccgaac aaaaatgagt
     1021 cttacgaa
//
LOCUS       DAZFQD010000342         1024 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.349, whole genome shotgun sequence.
ACCESSION   DAZFQD010000342 DAZFQD010000000
VERSION     DAZFQD010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1024
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.349"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            173..>1024
                     /locus_tag="ACHKTW_005332"
     CDS             173..>1024
                     /locus_tag="ACHKTW_005332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element IS621 family transposase"
                     /protein_id="HFO6466164.1"
                     /translation="MEHELHYIGIDTAKEKLDVDVLRPDGRHRTKKFANTTKGHDELV
                     SWLKGHKIDHAHICIEATGTYMEPVAECLYDAGYIVSVINPALGKAFAQSEGLRNKTD
                     TVDARMLAEFCRQKRPAAWEAPHPLERALRALVVRHQALTDMHTQELNRTETAREVQR
                     PSIDAHLLWLEAELKRLEKQIKDLTDDDPDMKHRRKLLESIPGIGEKTSAVLLAYIGL
                     KDRFAHARQFAAFAGLTPRRYESGSSVRGASRMSKAGHVSLRRALYMPAMVATSKTEW
                     GRAFRDRL"
BASE COUNT          246 a          256 c          295 g          227 t
ORIGIN      
        1 agagaaaatc ggccagtttt ctctgcctgc agtccgcatg ccgtatcggg ccttgggttc
       61 taacctgttg cgtagattta tgcagcggac tgcctttctc ccaaagtgat aaaccggaca
      121 gtatcatgga ccggttttcc cggtaatccg tatttgcaag gttggtttca ctatggaaca
      181 tgaacttcat tatatcggta tcgacaccgc taaagagaaa ctggatgtcg atgtgttgcg
      241 tcctgatggt cgtcatcgca ccaaaaaatt cgctaacacc actaaagggc acgatgagct
      301 ggtgagctgg ctgaaaggtc acaagattga ccatgcgcat atctgcatcg aagcgaccgg
      361 cacctatatg gaacctgtcg ctgagtgcct ttacgatgct ggctacatag tgtcagtcat
      421 taatcctgcg ctgggtaaag ctttcgctca gagtgaagga ctgcgtaaca agactgatac
      481 cgtggatgcg cgcatgctgg cagagttctg tcgtcagaag cgccctgcag cctgggaagc
      541 gcctcacccg cttgaacgcg cgttgcgtgc cctggtagtc cgccaccagg cgctgacaga
      601 tatgcacacg caggaactga atcgcactga aacggcgcgg gaagtccaga gaccgagcat
      661 tgatgctcac cttctgtggc ttgaagcaga gctgaagcgt cttgagaagc agataaaaga
      721 cctgacagac gatgatccgg atatgaaaca ccgcaggaaa ctgctggaaa gcatcccggg
      781 tatcggagag aaaacatctg cggtattgct ggcttatatc ggtctgaagg accgcttcgc
      841 ccatgccaga cagttcgccg cttttgcggg tctgacacca cggcgttatg aatcaggtag
      901 cagtgtgaga ggggcgagcc ggatgagtaa ggccggacat gtgtcgcttc gcagggcgtt
      961 gtatatgccc gcaatggtag ccaccagtaa gactgagtgg ggacgggcgt tccgcgaccg
     1021 tctg
//
LOCUS       DAZFQD010000343         1022 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.350, whole genome shotgun sequence.
ACCESSION   DAZFQD010000343 DAZFQD010000000
VERSION     DAZFQD010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1022
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.350"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            583..789
                     /locus_tag="ACHKTW_005333"
     CDS             583..789
                     /locus_tag="ACHKTW_005333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000233586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor protein"
                     /protein_id="HFO6466165.1"
                     /translation="MVHQHYGTQTVNRGAVRPGMLVKHKDGTWTASANLRGRLYLHRG
                     IERTYTRDLLVEVFLDGRGNGLNR"
     gene            865..>1022
                     /locus_tag="ACHKTW_005334"
     CDS             865..>1022
                     /locus_tag="ACHKTW_005334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0060703 - deoxyribonuclease inhibitor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HFO6466166.1"
                     /translation="MNAYYIQDRLEAQSWARHYQQIAREEKEAELADDMEKGLPQHLF
                     ESLCIDHL"
BASE COUNT          283 a          272 c          222 g          245 t
ORIGIN      
        1 ctcaatatat gatcggagat gccataaaaa ccatattcga aattgagcat gtaaaaaaac
       61 agttgaatcc tcaaaggcaa atagatcttc cattcgaata aagatacccg gccaccgtgc
      121 cgggttttct tttgtcccct cccctcatca cacaaaccgt tcgaaaaacc accacaacct
      181 cccttcagtt atcgctatgc gatgcaagtc acaaaattaa ttctttttgc tatcaaacag
      241 ttaatatcaa aacacatcaa tcaatagcaa tagctattga taccaccaat agcaatagct
      301 attatcacca tgtcgcaaca acacaacgat acggcaacca cctgattcac cgttgcgatg
      361 accgcttaga tccgcagctt gaatttcggc aggctccggg gagtgcgagg ggtgaagcgg
      421 acgcgtgaac gtcggtgtga ccagctgaaa tcaacacaac actttatacc tcagtcgctt
      481 caacgaggcg gcttagttat gacaaccggc ggccatccac cgcctgaata cgcgcagaag
      541 tctctatatg ttcagcagcc cagcttacgg gcaggagttt ttatggttca tcaacattac
      601 ggaactcaga ccgttaatcg cggtgccgtc cggccaggta tgctggttaa acacaaagat
      661 ggtacctgga cagcatcagc taatttacgc ggacggcttt atctgcatcg cggcatcgag
      721 cgcacttata cccgtgattt gctcgtggaa gtttttctcg acggacgcgg taacggcctg
      781 aatcgctaat cccctttcct gttttcctaa tcagcctggc atttcgcggg cgatattttc
      841 acagccattt tcaggagttc agccatgaac gcttattaca ttcaggatcg tcttgaggct
      901 cagagctggg cgcgtcacta ccagcagatc gcccgtgaag agaaagaggc agaactggca
      961 gacgacatgg aaaaaggcct gccccagcac ctgtttgaat cgctatgcat cgatcatttg
     1021 ca
//
LOCUS       DAZFQD010000344         1019 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.351, whole genome shotgun sequence.
ACCESSION   DAZFQD010000344 DAZFQD010000000
VERSION     DAZFQD010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1019
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.351"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..190
                     /locus_tag="ACHKTW_005335"
                     /pseudo
     CDS             1..190
                     /locus_tag="ACHKTW_005335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309558.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1327 domain-containing protein"
     gene            613..>1019
                     /locus_tag="ACHKTW_005336"
     CDS             613..>1019
                     /locus_tag="ACHKTW_005336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO6466167.1"
                     /translation="MNAKIRYGLSAAILALITAGASAPDILDQFLDEKEGNHTTAYRD
                     GSGIWTICRGATVVDGKPVVPGMKLSKEKCDQVNAIERDKALAWVEKNIRVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYRRINAGDRRGA"
BASE COUNT          280 a          223 c          267 g          249 t
ORIGIN      
        1 gttttacgaa gctgaggcga aaaagaaggc gaagcagttt ttcatggata ttgctgccgg
       61 gttatgtgaa ggggatgagc agtcgccgga aaagcgcccc ataattttag aggcgcagga
      121 tgtgttgata acctacagag gaaaactacc gggaataatt actggttctc tgaagacgcc
      181 accgacggca ttgcggtcag aaaacgatga tattgaatca cgcattgaaa aactggagtg
      241 ctatatcgct gaattgaaaa aaagcacccc aacaaaaaat gaggtgcttg cagcagatga
      301 aatgaaagaa actattcttg atcgcgcggc gcatctaagc tgcgcttcac tgttgaaaga
      361 gcatcttcag cagccttgag gaatctttta tcatcacgag caactgtctt tggtatttct
      421 ttaattagat gttccatttc ggcgatcagg taatctgaca cagcgttatt tttacttagt
      481 gcatccattg ctttgattat ctttgcgagg cagccatctc gcattgttga gtttacgcca
      541 gtgcccacca ctggcgggct gaagacttaa catatccagg gattcggaac cgataaatcc
      601 tgataaatat ccatgaacgc aaaaatcaga tacggcctgt cggctgccat tctggcgctg
      661 attactgcag gcgcatctgc gcctgacatt ctcgaccagt ttctggatga aaaagaaggt
      721 aaccacacca cagcataccg tgatggttct ggtatatgga ccatctgccg gggtgccacg
      781 gtggtggatg gcaaacctgt cgttccgggc atgaagttgt cgaaggaaaa atgcgaccag
      841 gttaacgcca ttgagcgtga taaagcgctg gcgtgggtgg agaaaaacat cagagtgccg
      901 ctgaccgaac cccagaaagc ggggatcgcg tcattctgtc cgtacaacat tggtcccggt
      961 aagtgtttcc cgtcgacgtt ttacagacga attaatgctg gtgatcgcag gggagcatg
//
LOCUS       DAZFQD010000345         1012 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.352, whole genome shotgun sequence.
ACCESSION   DAZFQD010000345 DAZFQD010000000
VERSION     DAZFQD010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1012
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.352"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            263..338
                     /locus_tag="ACHKTW_005337"
     tRNA            263..338
                     /locus_tag="ACHKTW_005337"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:296..298,aa:Ile,seq:cat)
BASE COUNT          314 a          180 c          208 g          310 t
ORIGIN      
        1 cagctaatgt aataaagtta tattattaga tacaaaatca ttacaaacta tcattgaatc
       61 gatgataact atagatagtg ctaattatta acatttgctt gttgactgcg agtatcgact
      121 ttgcgaatgg aagcggtgtc gggttttatg tcgagtgtaa tgacgagcaa ggatgaatga
      181 gaacgcataa aaatctgtta tttacatcat atgatcatca tgtttattgc gtggtttata
      241 agtattgtta ttgcacactc aaggcccttt agctcagtgg ttagagcagg cgactcataa
      301 tcgcttggtc gctggttcaa gtccagcaag ggccaccaaa tcattattta cgtatcagga
      361 agagttaagg gtacaagggg tactcgatgg cggtctatat ttcatgacta acgccatttc
      421 aaggaagaag tgcggtgggt ggtttcgaat gagggctggt gttttggtac gtggcacgcc
      481 actgcaatgt atttatccat cagtactcat tcaaaagtac ctggaaaaaa tgcctgctat
      541 gccaagcggg caaattgctt acatgtggaa ttaagtaatt aacgataaag ccagagtatt
      601 atcatttatt atccccctta atttaatcag gttaattact caaatcactg aactattctc
      661 tgttcgctca ctgatgatga gtgagtacca caaccaatgt atgtagaaca atgccgtaga
      721 gtaaggatga aatgtctctg ccacgcctta aacatctact actgaccaga acacccgccg
      781 caggcagatg agttttgtga gcgttacagg agcgtggcta tgctcattaa ccaacgctat
      841 tgagatataa atttctaaca ctcgaaaaac gaatttcact aataaattat aacgggtggt
      901 ttttatcata cagcgaaata actttttgtt tattaaattt aacattcttg tgctaattta
      961 attaaaagta ccatgtatag catatgaagc tgttaatgcc agcaattgtc tc
//
LOCUS       DAZFQD010000346          993 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.353, whole genome shotgun sequence.
ACCESSION   DAZFQD010000346 DAZFQD010000000
VERSION     DAZFQD010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..993
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.353"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            279..869
                     /locus_tag="ACHKTW_005338"
     CDS             279..869
                     /locus_tag="ACHKTW_005338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFO6466168.1"
                     /translation="MSRIYAYCRVSTLEQTTKNQRREIESAGFNIKSQQIIEEQISGS
                     AATSERPGFNQLLDRLKRGDALIVTKLDRLGCNPMDISKTVEQLTKAGIKVHCLALGG
                     VDLTSPPGKMMMQVISAVAEFERDLLLERTHSGILRARGAGKRFGRPPVLNDEQIQRV
                     LEQIKSGINISDIAQEFKTSRQTILRAKAKNQTPDI"
BASE COUNT          328 a          205 c          193 g          267 t
ORIGIN      
        1 acattttgct ccactttttc acgaccaaac aacttgaaat ctggttaaaa taacacgcaa
       61 cactattctt cttccttgag tccccccggg actcgaaaaa caaaccgagt taaggccatt
      121 tttcacaaaa tcgattttgg gtctcaccaa aattacgggg ttgcatacgc attcgtttat
      181 tttcgaacgt gtacatacaa atatgtacaa aaataatcaa aattattttc tgagatgcat
      241 tatgatatgt acaccaattt cgtatagaag atctcactat gtctcgaatt tatgcttact
      301 gtcgggtatc aactctggaa cagacaacca aaaatcaacg tcgggaaatc gaaagtgcag
      361 gttttaacat caaatctcag caaataattg aagaacaaat tagcggctca gcagcaacca
      421 gtgagcgtcc tggttttaac cagttgcttg atcgactgaa aagaggtgat gcattgattg
      481 tcacaaaact ggatcgcctc ggttgtaatc caatggatat cagtaaaacg gtggaacaac
      541 tgaccaaagc aggtatcaaa gtgcattgct tagcattggg gggcgttgac ctgaccagtc
      601 cacctggaaa aatgatgatg caagtaattt cagcagtcgc tgaatttgag cgagaccttt
      661 tacttgagcg tactcattca gggatattaa gagcacgggg cgcaggaaag cgatttggtc
      721 gcccacctgt gttaaatgat gaacaaatac agagggtact agagcaaatt aagtcaggta
      781 taaatataag tgacattgcc caagaattca aaacctcgcg gcaaaccatt ttaagagcta
      841 aagcaaaaaa tcagacacct gatatataaa aataatctcg gtgtgagatt ccttacgttt
      901 tccaagcccc ccttcctagc cttaaatgga aagatacttc taattataga atttatatat
      961 cttaccctac ggtagggctg gccattcaat atc
//
LOCUS       DAZFQD010000347          989 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.354, whole genome shotgun sequence.
ACCESSION   DAZFQD010000347 DAZFQD010000000
VERSION     DAZFQD010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..989
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.354"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(211..441)
                     /gene="ynfN"
                     /locus_tag="ACHKTW_005339"
     CDS             complement(211..441)
                     /gene="ynfN"
                     /locus_tag="ACHKTW_005339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnfN"
                     /protein_id="HFO6466169.1"
                     /translation="MKKIIVFFNSEPAVVVPAMTGVNTIMREYPNGEKTHLTVMAAGF
                     PSLTGDHKVIYVAADRHVTSEEILEAAIRLLS"
     gene            complement(446..511)
                     /gene="ynfR"
                     /locus_tag="ACHKTW_005340"
     CDS             complement(446..511)
                     /gene="ynfR"
                     /locus_tag="ACHKTW_005340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small protein YnfR"
                     /protein_id="HFO6466170.1"
                     /translation="MKAPSGAFLLGVYSMDTHILR"
     gene            complement(514..702)
                     /gene="ynfQ"
                     /locus_tag="ACHKTW_005341"
     CDS             complement(514..702)
                     /gene="ynfQ"
                     /locus_tag="ACHKTW_005341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFO6466171.1"
                     /translation="MTNHIHFRCPCCHGSQYRTSSFDVSDMNPFGAKCIFCKSMMITF
                     DNISQYLNASRLSLDLKK"
     gene            complement(713..925)
                     /gene="cspI"
                     /locus_tag="ACHKTW_005342"
     CDS             complement(713..925)
                     /gene="cspI"
                     /locus_tag="ACHKTW_005342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspI"
                     /protein_id="HFO6466172.1"
                     /translation="MSNKMTGLVKWFNPEKGFGFITPKDGSKDVFVHFSAIQSNDFKT
                     LTENQEVEFGIENGPKGPAAVHVVAL"
BASE COUNT          314 a          197 c          173 g          305 t
ORIGIN      
        1 atctgcttct gcttttgttt ttaagtcttc aatattcatc atttcctctt ttagtctgtt
       61 atgactttcc agttacacag taagtcgatt atatggtgca aacgtgtaaa agataagatg
      121 aaacatcgca ataatcaaca tacgatagtc taaattttac acaaacagac aaagagaatt
      181 ttcctgaatt atcaatgcaa tagcatcaaa tcaactcaag agccttattg ctgcttccag
      241 aatttcttct gaagtgacat gtcgatccgc ggctacataa atgactttat gatctccggt
      301 cagagatgga aaccctgcgg ccattacagt aaggtgtgtt ttttcgccat ttggatattc
      361 acgcatgatg gtgttaactc cagtcatcgc tggcactacc actgctggtt cagagttaaa
      421 aaaaactatg atttttttca tgatgttacc gtagtatgtg agtatccatc gaatagacac
      481 caagcaaaaa agctcccgaa ggagccttca ttttcacttt tttaaatcca acgacagacg
      541 gctggcattt aagtattgtg aaatattatc aaatgtaatc atcattgatt tacaaaagat
      601 acattttgcc ccgaaaggat tcatgtcaga aacatcaaaa gatgatgttc tatactggga
      661 accatgacaa cacgggcatc taaagtgaat atggtttgta atattgtcta cctcaaagcg
      721 ccactacatg aacagcggca ggacctttag gtccgttctc aataccaaat tcaacttcct
      781 gattctcagt taatgttttg aaatcgttgc tctgaattgc tgagaaatgg acaaacacat
      841 ctttgctgcc atctttcggc gtgatgaaac caaaaccttt ttcagggtta aaccatttca
      901 ctaaaccagt cattttgtta gacataatta ttaccttttg aagaaattag cccttgggca
      961 gaatggtccg aaaaaaatat cagagagaa
//
LOCUS       DAZFQD010000348          989 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.355, whole genome shotgun sequence.
ACCESSION   DAZFQD010000348 DAZFQD010000000
VERSION     DAZFQD010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..989
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.355"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(97..636)
                     /locus_tag="ACHKTW_005343"
     CDS             complement(97..636)
                     /locus_tag="ACHKTW_005343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFO6466173.1"
                     /translation="MSNIGYKRVSTVDQNTDRQLAGVTLDKVFEDKLSGKDTNRPGLQ
                     ACLSYLREGDTLHVHSLDRLGRNTLDILALVEELNNRGVIVRFHKEGIIADITSAMGK
                     MMLTMLSAVATCERELMLERKREAQAVNPTKRGKGKAVDREGIKKALSDGLSVRKTAE
                     SFNVAPSTVQRIKKEEEEA"
BASE COUNT          278 a          230 c          188 g          293 t
ORIGIN      
        1 tcccttccct tatggctaga ttgcgagagg tgcttaacgc gattttcagc gccccccgat
       61 ctggaaaatc cagaatcccc aaatatcaag gaggcattac gcctcctctt cttccttctt
      121 gatacgttgc accgttgaag gagcaacatt aaagctctcg gcggttttac gcactgacag
      181 gccatcagaa agggctttct ttataccttc cctatctact gccttccctt taccacgctt
      241 tgtaggattg acggcttgcg cttctcgttt acgttctaac atcagttcac gttcacacgt
      301 tgccactgcc gacaacatcg taagcatcat ctttcccatc gcgcttgtaa tgtctgcaat
      361 gatcccttct ttgtgaaagc gaacaattac accacgatta ttaagctctt ccactaaagc
      421 aagaatgtct aaggtgttac gccctaaacg gtcgagagag tgcacgtgta aggtgtcgcc
      481 ttctctcaga taggataaac acgcctgcaa tcctggacgg ttagtgtctt taccagacag
      541 cttatcttca aagactttat cgagagttac tcctgcaagc tggcgatcag tgttctgatc
      601 tacagtgctc acacgcttat aaccgatatt gctcataaaa tcctcttaat gtatcgattt
      661 ggcactactt caatgcaaat gagtatatcg tattcatttg gtctgtttca agccttatag
      721 gtacggaata ttacacgatt tagatgtatt catttggtat gcctaatgga tacacagggg
      781 aatagattaa atggtgttct gtttatacag gtttatttgt tagatcaaca atttggcatt
      841 tctaaacaga tgtttgaacg ttgccaaatg tcttgacaac acaagaaaag ggcttgctat
      901 gccacgcatt acacgaaata aggctgtaaa aacaaaataa attttaaaat ttgcacaaac
      961 aagaaaactc gttcagaaac aagcagaac
//
LOCUS       DAZFQD010000349          986 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.356, whole genome shotgun sequence.
ACCESSION   DAZFQD010000349 DAZFQD010000000
VERSION     DAZFQD010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..986
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.356"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>986
                     /locus_tag="ACHKTW_005344"
     CDS             <1..>986
                     /locus_tag="ACHKTW_005344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO6466174.1"
                     /translation="YTNSDVVVDDNGVGFRYSFSALKDRHTAVEVNYTDPQNGWQTST
                     ELVEDPEAILRYGRNLLKMDAFGCTSRGQAHRAGLWVIKTGLLETQTVDFTLGSQGLR
                     HTPGDIIEICDNDYAGTLTGGRVLSIDAATRTLTLDREVTLPGTGTLTVNLINGSGKP
                     VSVDITAHPAPDRIQVSPLPDGVETYGVWGLSLPSLRRRLFRCVSIRENTDGTFAITA
                     VQHVPEKEAIVDNGARFEPQSGTLNSVIPPAVQHLTVEVSAADSQYLALAKWDTPRVV
                     KGVRFSLRLTSGSGENSRLLTTAITADTEHRFSGLPLGEYTLTVRAINSYGQ"
BASE COUNT          211 a          293 c          314 g          168 t
ORIGIN      
        1 tacaccaaca gcgatgtggt ggtggatgat aacggcgtgg ggttccgcta cagcttcagt
       61 gccctgaagg accggcacac ggcggtggag gtgaattaca ccgacccgca gaacggctgg
      121 cagacctcca cggaactggt ggaagacccg gaagccatac tgcgctacgg acgcaacctg
      181 ctgaagatgg acgcgttcgg ctgtaccagc cgcggtcagg cccaccgtgc cggactgtgg
      241 gtgataaaga ccggactgct ggaaacgcag acggtggatt tcacactcgg gtcacagggg
      301 ctgcggcaca cacccggtga cattattgaa atctgtgata atgactatgc cgggaccctg
      361 accggcggac gtgtcctgtc cattgatgct gccacccgca ccctgacgct ggaccgtgaa
      421 gtgacacttc cggggacagg tacattgacg gtgaacctga ttaacggcag cggtaagccg
      481 gtgagtgtgg acatcaccgc acaccccgcg ccggaccgga tacaggtcag ccccttgccg
      541 gatggcgtgg agacatacgg ggtgtgggga ctctccctgc cgtcactgcg ccgtcgcctg
      601 ttccgctgtg tctccatccg ggaaaacacg gacggcacct ttgccatcac ggcggtgcag
      661 cacgtaccgg aaaaagaagc cattgtggat aacggagccc gctttgagcc gcagtcaggc
      721 accctgaaca gcgttatccc accggcagta cagcacctca cggtggaggt tagcgcagct
      781 gacagccagt atctggcgct ggcgaaatgg gacacgccga gggtggtgaa gggcgtgcgc
      841 ttcagtctgc gcctgaccag tggaagcggt gagaacagcc gcctgctgac caccgccatc
      901 actgccgata cggagcaccg tttcagtggc ctgcctctcg gggaatacac cctgacggtc
      961 agggcgatta acagctatgg ccagca
//
LOCUS       DAZFQD010000350          971 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.357, whole genome shotgun sequence.
ACCESSION   DAZFQD010000350 DAZFQD010000000
VERSION     DAZFQD010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..971
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.357"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..152)
                     /gene="mchI"
                     /locus_tag="ACHKTW_005345"
     CDS             complement(<1..152)
                     /gene="mchI"
                     /locus_tag="ACHKTW_005345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin H47 immunity protein MchI"
                     /protein_id="HFO6466175.1"
                     /translation="MSYKKLSQLTAIFSLPITILLVSLSSLRIVGEGNSYVDVFLSFI
                     IFLGFI"
     gene            565..810
                     /locus_tag="ACHKTW_005346"
     CDS             565..810
                     /locus_tag="ACHKTW_005346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6466176.1"
                     /translation="MNCDNNHRNEEFIVTFDKGNKQDNSRRKHDNFPIEVESSVEQET
                     HCITNNKSASGIVTHDYDADYICGCGEIMCPGCGHDL"
BASE COUNT          352 a          170 c          176 g          273 t
ORIGIN      
        1 tcaataaaac caagaaatat tataaagctt agaaaaacgt caacataaga attcccttcg
       61 ccaacaatcc gaagggatga aagtgaaacc aataagatag taataggtaa actgaatata
      121 gccgtcaatt gggacagttt tttataactc attaatccct acctgttgct ttattttcat
      181 tatgaaaagt taccaaatct tagcacctca gtcaggagag tcaggtcatc gataaagaat
      241 attccagtag agtggcgctc atgatcaaag ataacagcaa agttgatgac agtgctgacc
      301 gactgtcatt tacagaaacc ctgtttgtag cacattccat cttacctgac ctcacccaga
      361 caaagacaaa agagaatgat tgttatttac ttttcaaaaa aaagtgtcat attgtccata
      421 taaagtcaag aggtatttta cgttatgtca ctgatgcaat tgttagtcag gatctcatgg
      481 ttgtcgctca tgtataacct gagacttgaa cgcacactaa taacactgac ggtcatacat
      541 cgggaaatct gaggaggcat aagaatgaat tgtgataata atcacagaaa tgaagaattc
      601 attgttacct ttgataaagg caacaagcaa gacaattcaa gacgaaaaca cgataatttt
      661 cctatagagg tagaatcctc cgtagagcag gagacacact gtatcacaaa taataagtcg
      721 gcttccggta tagtaacaca tgactatgat gccgattata tttgtggttg tggtgaaatt
      781 atgtgtcctg gttgcggtca tgacctataa caaacatttt tgcaaactga tacctgatca
      841 ggtacaagcc tgttgattat tatgctgaat atttcatgtt ataaaaccat cgatataaac
      901 attaacaaga aaaaacatat attgtttatc tgaacattta tcgatggaaa accaaaggga
      961 aaagtatgta t
//
LOCUS       DAZFQD010000351          962 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.358, whole genome shotgun sequence.
ACCESSION   DAZFQD010000351 DAZFQD010000000
VERSION     DAZFQD010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..962
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.358"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..312
                     /locus_tag="ACHKTW_005347"
     CDS             <1..312
                     /locus_tag="ACHKTW_005347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0042262 - DNA protection [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HFO6466177.1"
                     /translation="DWCKTNRNELTNGGETKTANLTTGTVSWRLGNPSCSVSRDVEGV
                     IEMLRRMGLERFIRTKEEVNKQAVLAEPDAVKGIAGIKVNKGAENFHVEPFEQDAGLN
                     K"
     gene            397..>962
                     /locus_tag="ACHKTW_005348"
     CDS             397..>962
                     /locus_tag="ACHKTW_005348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012908585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5420 family protein"
                     /protein_id="HFO6466178.1"
                     /translation="MSIKYRYFKMNESDSRHYHREWLDCVGRERQKLIDDFLSNQNAR
                     GYCYSWYCGDFFVKSILVHEDVDVGRNKRVLSDKFDEDGRTLFSVKPDRRFREGKKLN
                     KALNILNEKLKQLPSFSTWMVNKLDCYFEVFGVSNGRTVMACSSAGFYGDKGAVVVRI
                     PVGESDNAFSPEKLHPSLMAIKHSEFIA"
BASE COUNT          267 a          178 c          257 g          260 t
ORIGIN      
        1 gactggtgta aaaccaaccg caatgagctg acgaacggcg gtgaaaccaa aactgccaat
       61 ctgaccaccg gaacggtgtc atggcggctg ggaaatccat catgcagcgt cagtcgtgat
      121 gtggaaggtg tgattgaaat gctgcgtcgt atggggcttg agcgcttcat ccgcacgaaa
      181 gaggaagtga ataagcaagc tgtcctggca gagccggatg cggtgaaagg gattgcgggt
      241 attaaggtga ataaaggcgc tgaaaatttt catgtcgagc cttttgaaca ggacgccgga
      301 ctgaataaat aacaccgcat taaatcttta aatatcacat cgttttaatt atggcgctcg
      361 cgtcagggga ctgctcgcgc ctgaatgagg catgtcatga gtattaaata tcgttatttt
      421 aaaatgaatg agagcgattc ccgtcattat cacagggaat ggcttgattg tgttggcagg
      481 gagcgccaga aattgattga tgattttctc agtaatcaga atgccagagg gtattgttat
      541 tcctggtatt gtggggattt ttttgtcaaa tcaatactgg tgcatgagga tgttgatgtc
      601 ggaagaaata aacgggtgtt gtctgataaa tttgatgaag atggccgtac tttattttca
      661 gttaagcccg accgcagatt cagagaggga aaaaaactga acaaagcact gaatatctta
      721 aacgagaagt taaaacagct tccgtcattc agtacctgga tggttaataa actggactgc
      781 tattttgagg tttttggtgt cagcaacggc cgcacagtta tggcgtgctc ttctgccggg
      841 ttctatggcg acaaaggggc tgttgtggtt cgtattcctg tcggtgaatc agataatgcc
      901 ttttcacctg aaaagctgca tccgtcattg atggcaatca agcattccga attcatcgca
      961 at
//
LOCUS       DAZFQD010000352          938 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.359, whole genome shotgun sequence.
ACCESSION   DAZFQD010000352 DAZFQD010000000
VERSION     DAZFQD010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..938
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.359"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            174..>938
                     /locus_tag="ACHKTW_005349"
     CDS             174..>938
                     /locus_tag="ACHKTW_005349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ESPR-type extended signal peptide-containing
                     protein"
                     /protein_id="HFO6466179.1"
                     /translation="MNRIYRVIWNCTLQVFQACSELTRRAGKTSTVNLRKSSGLTTKF
                     SRLTLGVLLALSGSASGASLEVDNDQITNIDTDVAYDAYLVGWYGTGVLNILAGGNAS
                     LTTITTSVIGANEDSEGTVNVLGGTWRLYDSGNNARPLNVGQSGTGTLNIKQKGHVDG
                     GYLRLGSSTGGVGTVNVEGEDSVLTTELFEIGSYGTGSLNITDKGYVTSSIVAILGYQ
                     AGSNGQVVVEKGGEWLIKNNDSSIEFQIGNQGMGEAT"
BASE COUNT          256 a          173 c          239 g          270 t
ORIGIN      
        1 ctatgcaatt tttaatctat caattcgaaa gcattagcaa attagccaaa ttgacacctt
       61 cccacctcca actaacactc aatacagacc tctgacaatt taataagttt catttttaat
      121 gtctgacgac tcatctcttt cgtggctgta ttgtttgtat atggagcgtt tatatgaata
      181 gaatctatcg cgtgatatgg aattgcactc tacaggtatt tcaggcctgc tcggaattaa
      241 ctcgcagggc aggtaaaaca tcgacggtta atttgcgtaa atcctctgga ctgacaacga
      301 aattcagtag attgacgctg ggtgttttgc tggcactaag cggttcagcg tctggtgcaa
      361 gtctggaagt tgataatgat cagattacca atattgatac tgatgttgct tatgatgcct
      421 acctggttgg ctggtatggc actggagtgc ttaatatttt ggctggcggt aatgcctcct
      481 taaccactat tactaccagc gtcattggcg ctaatgagga ctcagagggc accgttaatg
      541 ttttgggtgg cacctggcga ttgtatgata gcggaaataa tgcaaggcct ttaaatgtgg
      601 gtcaatccgg aacggggacg ctgaatatta aacagaaggg tcacgtcgat ggaggctatt
      661 taagattagg ttcttcgaca ggaggcgtcg ggacggtcaa tgttgaggga gaggactctg
      721 ttctgacgac cgaattattc gaaataggga gttacggtac gggctcatta aatattacgg
      781 ataagggtta cgtcacgagt tcaatcgtcg ccattttagg ctatcaggcg ggcagtaatg
      841 gccaggttgt cgttgaaaag ggtggcgagt ggctaataaa aaataacgat tcctcaattg
      901 aatttcaaat tggtaatcaa gggatggggg aagcgact
//
LOCUS       DAZFQD010000353          914 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.360, whole genome shotgun sequence.
ACCESSION   DAZFQD010000353 DAZFQD010000000
VERSION     DAZFQD010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..914
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.360"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>914
                     /gene="tuf"
                     /locus_tag="ACHKTW_005350"
     CDS             <1..>914
                     /gene="tuf"
                     /locus_tag="ACHKTW_005350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFO6466180.1"
                     /translation="AHVDCPGHADYVKNMITGAAQMDGAILVVAATDGPMPQTREHIL
                     LGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQYDFPGDDTPIVRGSALKAL
                     EGDAEWEAKILELAGFLDSYIPEPERAIDKPFLLPIEDVFSISGRGTVVTGRVERGII
                     KVGEEVEIVGIKETQKSTCTGVEMFRKLLDEGRAGENVGVLLRGIKREEIERGQVLAK
                     PGTIKPHTKFESEVYILSKDEGGRHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMP
                     GDNIKMVVTLIHPIAMDDGLRFAIREGGR"
BASE COUNT          212 a          228 c          264 g          210 t
ORIGIN      
        1 gcacacgtag actgcccggg gcacgccgac tatgttaaaa acatgatcac cggtgctgct
       61 cagatggacg gcgcgatcct ggtagttgct gcgactgacg gcccgatgcc gcagactcgt
      121 gagcacatcc tgctgggtcg tcaggtaggc gttccgtaca tcatcgtgtt cctgaacaaa
      181 tgcgacatgg ttgatgacga agagctgctg gaactggttg aaatggaagt tcgtgaactt
      241 ctgtctcagt acgacttccc gggcgacgac actccgatcg ttcgtggttc tgctctgaaa
      301 gcgctggaag gcgacgcaga gtgggaagcg aaaatcctgg aactggctgg cttcctggat
      361 tcttatattc cggaaccaga gcgtgcgatt gacaagccgt tcctgctgcc gatcgaagac
      421 gtattctcca tctccggtcg tggtaccgtt gttaccggtc gtgtagaacg cggtatcatc
      481 aaagttggtg aagaagttga aatcgttggt atcaaagaga ctcagaagtc tacctgtact
      541 ggcgttgaaa tgttccgcaa actgctggac gaaggccgtg ctggtgagaa cgtaggtgtt
      601 ctgctgcgtg gtatcaaacg tgaagaaatc gaacgtggtc aggtactggc taagccgggc
      661 accatcaagc cgcacaccaa gttcgaatct gaagtgtaca ttctgtccaa agatgaaggc
      721 ggtcgtcata ctccgttctt caaaggctac cgtccgcagt tctacttccg tactactgac
      781 gtgactggta ccatcgaact gccggaaggc gtagagatgg taatgccggg cgacaacatc
      841 aaaatggttg ttaccctgat ccacccgatc gcgatggacg acggtctgcg tttcgcaatc
      901 cgtgaaggcg gccg
//
LOCUS       DAZFQD010000354          903 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.361, whole genome shotgun sequence.
ACCESSION   DAZFQD010000354 DAZFQD010000000
VERSION     DAZFQD010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..903
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.361"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            49..834
                     /locus_tag="ACHKTW_005351"
     CDS             49..834
                     /locus_tag="ACHKTW_005351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFO6466181.1"
                     /translation="MNSKKLCCICVLFSLLAGCASESSIDEKKKKAQVTQSNINKNTP
                     QQLTDKDLFGNETTLAVSEEDIQAALDGDEFRVPLNSPVILVQSGNRAPETIMQEEMR
                     KYYTVSTFSGIPDRQKPLTCNKNKDKNENEDVASAENMNWMQALRFVAAKGHQKAIIV
                     YQDTLQTGKYDSALKSTVWSDYKNEKLTDAISLRYLVRFTLVDVATGEWATWSPVNYE
                     YKVLPPLPDKNEASTTDMTEQQIMQLKQKTYKAMVKDLVNRYQ"
BASE COUNT          297 a          184 c          192 g          230 t
ORIGIN      
        1 caatgctaaa tattctaatc attatgacag gcgagggagt gtccaattat gaattcaaaa
       61 aagctttgtt gcatatgtgt gttattctcg ctgcttgcag gatgtgcctc tgaatcttct
      121 attgatgaaa agaagaaaaa agctcaagtc acacaaagta atattaataa aaacactccc
      181 cagcaactga cagacaaaga tttattcggt aatgaaacca ctctggccgt atccgaagag
      241 gatattcaag ctgcgcttga tggagatgag ttccgcgttc cccttaattc tcctgtaata
      301 cttgttcaat ccggcaaccg cgcaccggaa accattatgc aggaggagat gcgaaaatat
      361 tatactgttt ccacattctc tggtatcccg gacaggcaaa agcctctgac ttgtaacaaa
      421 aacaaagata aaaatgagaa cgaggatgtt gccagtgctg agaatatgaa ctggatgcag
      481 gcactgcgtt ttgtggctgc aaaaggacat cagaaagcga ttattgttta ccaggataca
      541 ttgcagacag gaaaatatga ctctgcgctg aaatcaacag tatggtccga ctataaaaat
      601 gaaaaactca ccgacgctat ctccctgcgc tacctggtac gtttcacgct ggtggatgtg
      661 gcaacaggtg agtgggctac ctggtcgccg gtgaattatg aatataaagt gcttccacca
      721 ttgcccgaca agaatgaagc cagtacgact gatatgacag agcagcaaat catgcaactt
      781 aagcaaaaga cctataaagc gatggtaaaa gatttggtta accgctatca ataataaatt
      841 atatctgccg ccaggaattt ctggcggcaa taatacaaaa ttctttgcat aagaaatttt
      901 tct
//
LOCUS       DAZFQD010000355          900 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.362, whole genome shotgun sequence.
ACCESSION   DAZFQD010000355 DAZFQD010000000
VERSION     DAZFQD010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..900
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.362"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..568
                     /locus_tag="ACHKTW_005352"
                     /pseudo
     CDS             1..568
                     /locus_tag="ACHKTW_005352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001470092.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat domain-containing protein"
     gene            601..861
                     /locus_tag="ACHKTW_005353"
     CDS             601..861
                     /locus_tag="ACHKTW_005353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000889444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6466182.1"
                     /translation="MLEHFGAEASVLDMTIIVRSNPSKAAILEEFLHGTQEKLGIAEK
                     LGRYGLGSAETHVKDFMIRHKKMLGLSDEDVAILKILKDKGL"
BASE COUNT          245 a          190 c          261 g          204 t
ORIGIN      
        1 aactgtacag cgtacggcac ctggtgttta acggctttga gctgataagc cagcagttca
       61 gccgggtacg gcagccgcat ccgtccgtgg ccccgcagtg ggtgacgcgg acgaatcatg
      121 cggtgagcga cctgacgggc cgtccgctga tgctctttaa cagtgaaggt aaaaccgtct
      181 ggcgaccggg cagaccagcc tgtgggggct ggcactcagc ctgcccgcag acaccggcta
      241 cccggacccg cgcggggaac tggacccgga agccgacccc ggcctgctgt atgcggggca
      301 gtggcgggat gcggaatcgg ggctgtgcta taaccggttc cggtattacg agccggagag
      361 cgggatgtac ctagtgagtg atccactggg gttgcaggga ggggagcaga cttaccggta
      421 tgtgccgaat cctttaagat ggatagatcc cttaggatta aataaaggag cttcattatc
      481 taaaatgatg aatagctcca gtgatctcat ggggttgaga aggcagcccc agaacttctg
      541 gcggctatat cgcggaaaag acatttaata atagcattac caggctcaga tgaattcaga
      601 atgcttgaac attttggagc cgaagcttcg gtattagaca tgacgattat tgttcgttca
      661 aatccgagta aagccgctat tcttgaagag tttttacatg gtacacaaga gaaactagga
      721 atagctgaaa aattaggacg ttatggacta gggagtgcag aaacgcacgt taaggatttc
      781 atgataagac acaaaaaaat gcttgggtta tctgatgagg atgtagcaat actcaaaata
      841 ttgaaagaca aggggttgtg atgaatataa ttctgccgat aaaagatata tttattattg
//
LOCUS       DAZFQD010000356          895 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.363, whole genome shotgun sequence.
ACCESSION   DAZFQD010000356 DAZFQD010000000
VERSION     DAZFQD010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..895
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.363"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..100
                     /locus_tag="ACHKTW_005354"
     CDS             <1..100
                     /locus_tag="ACHKTW_005354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0060703 - deoxyribonuclease inhibitor
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HFO6466183.1"
                     /translation="DDDVEFQERMAEHIRYMVETIAHHQVDIDSEV"
     gene            106..891
                     /gene="bet"
                     /locus_tag="ACHKTW_005355"
     CDS             106..891
                     /gene="bet"
                     /locus_tag="ACHKTW_005355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage recombination protein Bet"
                     /protein_id="HFO6466184.1"
                     /translation="MSTALATLAGKLAERVGMDSVDPQELITTLRQTAFKGDASDAQF
                     IALLIVANQYGLNPWTKEIYAFPDKQNGIVPVVGVDGWSRIINENQQFDGMDFEQDNE
                     SCTCRIYRKDRNHPICVTEWMDECRREPFKTREGREITGPWQSHPKRMLRHKAMIQCA
                     RLAFGFAGIYDKDEAERIVENTAYTAERQPERDITPVNDETMQEINTLLIALDKTWDD
                     DLLPLCSQIFRRDIRASSELTQAEAVKALGFLKQKATEQKVAA"
BASE COUNT          244 a          227 c          234 g          190 t
ORIGIN      
        1 tgatgacgat gttgagtttc aggagcgcat ggcagaacac atccggtaca tggttgaaac
       61 cattgctcac catcaggttg atattgattc agaggtataa aacggatgag tacagcactc
      121 gcaacgctgg cagggaagct ggctgaacgt gtcggcatgg attctgtcga cccacaggaa
      181 ctgatcacca ctcttcgcca gacggcattt aaaggcgatg ccagcgatgc gcagttcatc
      241 gcattgttga tcgtcgccaa ccagtacggc cttaatccgt ggacgaaaga aatttacgcc
      301 ttccctgata agcagaacgg catcgttccg gtggtgggcg ttgatggctg gtcccgcatc
      361 atcaatgaaa accagcagtt tgatggcatg gactttgagc aggacaatga atcctgtaca
      421 tgccggattt accgcaagga ccgtaatcat ccgatctgcg ttaccgaatg gatggatgaa
      481 tgccgccgcg aaccattcaa aacccgcgaa ggcagagaaa tcaccggacc gtggcagtcg
      541 catcccaaac ggatgttacg gcataaagcc atgattcagt gtgcccgtct ggccttcgga
      601 tttgctggta tctatgacaa ggatgaagcc gagcgcattg tcgaaaatac tgcatacact
      661 gcagaacgtc agccagaacg cgacatcact ccggttaacg atgaaaccat gcaggagatt
      721 aacactctgc tgatcgccct ggataaaaca tgggatgacg acttattgcc gctctgttcc
      781 cagatatttc gccgcgacat tcgcgcatcg tcagaactga cacaggccga agcagtgaaa
      841 gctcttggat tcctgaaaca gaaagccact gagcagaagg tggcagcatg acacc
//
LOCUS       DAZFQD010000357          892 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.364, whole genome shotgun sequence.
ACCESSION   DAZFQD010000357 DAZFQD010000000
VERSION     DAZFQD010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..892
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.364"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            78..545
                     /locus_tag="ACHKTW_005356"
     CDS             78..545
                     /locus_tag="ACHKTW_005356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFO6466185.1"
                     /translation="MNRVLCVVIIVLLVACGALSLGLNHYRDNAITYKAQRDKKAREL
                     ELANATITDMQVRQRDVAALDAKYSRELADARAENETLRADVAAGRKRLRINATCHGT
                     VREATGTSGVDNATGPRLADTAERDYFTLRERLMLMQKQLEGAQDYIRTQCLN"
     gene            722..>892
                     /locus_tag="ACHKTW_005357"
     CDS             722..>892
                     /locus_tag="ACHKTW_005357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFO6466186.1"
                     /translation="MNYPTIVDGIDFRQLVTAADGEPITDSFQIAKAFGKRHADVLRA
                     LKNCHCSEDFRRA"
BASE COUNT          208 a          215 c          255 g          214 t
ORIGIN      
        1 actaatgctg gttgctattt ttctttccct gggctggatg atgttgtctt cgtcggggat
       61 gctggggtga gggggatatg aaccgtgttc tgtgtgtggt gattattgtc ctgctggtgg
      121 cctgtggtgc gcttagtctg gggctgaatc attaccgcga taacgccatc acctacaaag
      181 cgcagcgaga taaaaaagcc agagagctgg agctggcaaa cgcaaccatt actgatatgc
      241 aggtgcgcca gcgcgatgtt gctgcgctcg atgcaaaata ctcgagggaa ttagctgatg
      301 cgagagctga aaatgaaact ctgcgtgctg atgttgccgc tggtcgtaag cgcctgcgga
      361 tcaacgccac ctgccacggt accgtgcgtg aagccaccgg cacctccggc gtggataatg
      421 caaccggccc ccgactggca gacaccgctg aacgggatta tttcaccctc agagagcggt
      481 tgatgctgat gcagaagcag ctggaagggg cacaggacta tatccgcact cagtgcctga
      541 attaacagag ccagcttaat cgctggcttt ttcatatctg aatttcaccg cgcatctcac
      601 gcgcatatca catccccgag cctttcagaa agttgagcct gagaactgcc gtatatggtg
      661 gcgaccatct cggggcggct tttctgtgag acaggctcaa ttttctgaaa ggtaaaacgc
      721 gatgaactat ccgacgattg ttgacggtat tgattttcgc caactggtaa cagcggcaga
      781 tggcgaacca ataacagact cttttcagat cgctaaggcg tttggtaagc gtcatgcgga
      841 cgtattgagg gcgctgaaaa attgccattg ctctgaagat ttccggagag cg
//
LOCUS       DAZFQD010000358          883 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.365, whole genome shotgun sequence.
ACCESSION   DAZFQD010000358 DAZFQD010000000
VERSION     DAZFQD010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..883
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.365"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>883
                     /locus_tag="ACHKTW_005358"
     CDS             <1..>883
                     /locus_tag="ACHKTW_005358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HFO6466187.1"
                     /translation="AGRPEMRYRYDDTGRVVEQLNPAGLSYRYQYEQDRITVTDSLNR
                     REVLHTEGGAGLKRVVKKELADGSVTHSGYDAAGRLTAQTDAAGRRTEYGLNVVSGDI
                     TDITTPDGRETKFYYNDGNQLTAVVYPDGLESRREYDEPGRLVSETSRSGETVRYRYD
                     DAHSELPATTTDATGSTRQMTWSRYGQLLAFTDCSGYQTRYEYDRFGQMTAVHREEGI
                     SLYRHYDNRGRLTSVKDAQGRETQYEYNAAGDLTAVITPDGNRSETQYDAWGKAVSTT
                     QGGLTRSMEYDAAGRVI"
BASE COUNT          205 a          244 c          316 g          118 t
ORIGIN      
        1 acgcgggaag gccggagatg cgctaccgct acgacgatac cgggcgggtg gtggagcagc
       61 tgaacccggc aggcctgagt taccgctacc agtatgagca ggaccgcatc accgtcacgg
      121 acagcctgaa ccggcgtgag gtgctgcata cagaaggcgg ggccgggctg aagcgggtgg
      181 tgaaaaaaga actggcggac ggcagcgtca cgcacagcgg gtatgacgcg gcaggaaggc
      241 tcacggcgca gacggacgcg gcgggacggc ggacagagta cggtctgaat gtggtgtccg
      301 gcgatatcac ggacatcacc acaccggacg ggcgggagac gaaattttac tataacgacg
      361 ggaaccagct gacggcggtg gtgtacccgg acgggctgga gagccgccgg gaatatgatg
      421 aaccgggcag gctggtatcg gagacatcgc gcagcgggga gacagtacgc taccgctacg
      481 atgacgcgca cagtgagtta ccggcgacga caacggatgc gacgggcagc acccggcaga
      541 tgacgtggag ccgctacggg cagttgctgg cgttcactga ctgctcgggc taccagaccc
      601 gctatgaata cgaccgcttc ggccagatga cggcggttca ccgcgaggaa ggtatcagcc
      661 tttaccgcca ctatgacaac cgtggccggt taacctcggt gaaagacgca cagggccgtg
      721 aaacgcagta tgagtacaac gccgcaggcg acctgactgc cgttatcacc ccggacggca
      781 accggagcga gacacagtac gatgcgtggg gaaaggcggt cagcaccacg cagggcgggc
      841 tgacgcgcag tatggagtac gatgctgccg gacgtgtcat cag
//
LOCUS       DAZFQD010000359          882 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.366, whole genome shotgun sequence.
ACCESSION   DAZFQD010000359 DAZFQD010000000
VERSION     DAZFQD010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..882
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.366"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..111
                     /locus_tag="ACHKTW_005359"
     CDS             <1..111
                     /locus_tag="ACHKTW_005359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001824829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HFO6466188.1"
                     /translation="TKGLEIHVRQRTMKKEIEALDDAEAILAYKVGMADR"
BASE COUNT          285 a          151 c          212 g          234 t
ORIGIN      
        1 accaaaggtc tggaaattca tgtcagacag cgcacgatga aaaaagagat tgaagcgctg
       61 gatgatgcgg aagctatact ggcgtataag gtcggcatgg ctgacaggtg aaaaaaagag
      121 gggcgctcca gccccttgct acaaagctat ggctaaataa agaatactca ggtcgaggaa
      181 ataatctggc aatgataatt aaaggagatg caacggcctc cttcacccct gtcaaagata
      241 aaaaactggg gagtgcgtgc tccccgagag catgatactt gtggttgata gttattttta
      301 tccggcgaca aagatattga gatgaggtcg cggatgcaat acagaatttt cgtattgcat
      361 ggtaactgct gattcctgaa actgaaagtc gtcatgaaac tgtaagcgaa aaaacggggg
      421 gacggcacgc tccccaaggt catacatgag atagtggtgt ttttatagtc gaaagaataa
      481 ttgcataaaa agagtgtaaa ttaaacattc aggggataca gaattttcgt aggctgaggg
      541 attgctgaaa ataatttagt gcgtccttca atggtcattc aatggtcatc agattgtgct
      601 gatgtaaaca gttaaacaca atttgattat ttgactttct tctttttgtg actgacttca
      661 caattaaaga tgtaaccgta tacaaattgt tgatgaataa agagacagca taagtggcag
      721 gccagtcgta atgcggatat cccttcgcgg acgtgatatc accgacgtta tagtaaggaa
      781 attccgaaaa acttcatatt aaaattcgga atttcttcag gagtaagctc atgagagcgg
      841 cgagacatca gattttcttc atatatctga tgttgaactg tt
//
LOCUS       DAZFQD010000360          874 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.367, whole genome shotgun sequence.
ACCESSION   DAZFQD010000360 DAZFQD010000000
VERSION     DAZFQD010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..874
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.367"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>874
                     /locus_tag="ACHKTW_005360"
     CDS             <1..>874
                     /locus_tag="ACHKTW_005360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO6466189.1"
                     /translation="QEQTPPEGFESSGAETALGVEVTKAKPVTRTITSANIDRLRVTF
                     GVQSLVETTSKGDRNPSSVRLLIQLERNGNWVTEKDVTINGKTTSQYLTSVILNNLPE
                     RPFNIRMVRVTADSTTDQLQNRTLWSSYTEIIDVKQCYPNTAIVGLQVDAEQFGGQQM
                     VVNYHIRGRIIQVPSNYDPEKRTYSGIWDGSLKPAYSNNPAWCLWDMLTHPRYGMGKR
                     LGAADVDKWALYAIGQYCDQTVPDGFGGTEPRMTFNAYLSQQRKVWDVLGDFCSAMRC
                     MPVWNGQTLTFVQDR"
BASE COUNT          193 a          236 c          284 g          161 t
ORIGIN      
        1 agcaggagca gacaccgccg gaaggctttg agtcctccgg ggcggaaacc gcactgggcg
       61 tggaggtgac gaaggcaaag ccggtgacgc gcaccatcac gtcagcgaac attgaccgtc
      121 tgcgggtcac cttcggggtg cagtcactgg tggagaccac ctcaaagggt gaccgtaatc
      181 cctcttctgt ccggctgctg attcagcttg agcgtaacgg taactgggtg acggagaagg
      241 atgtcaccat taacggcaag accacctcgc agtacctgac gtcggtgatt ctgaataatc
      301 tccctgagcg cccctttaat atccggatgg tcagggtgac ggcggacagc accacggacc
      361 agctgcagaa cagaacgctg tggtcgtcat acaccgaaat catcgatgtg aaacagtgct
      421 acccgaacac ggccattgtg gggctgcagg tggatgcgga gcagttcggt ggccagcaga
      481 tggtggtgaa ctaccatatc cgcggccgca tcattcaggt gccgtcaaac tatgacccgg
      541 aaaaacgcac ctacagtggt atctgggacg ggagtctgaa accggcatac agcaataacc
      601 cggcctggtg cctgtgggac atgctgaccc acccgcgcta cgggatggga aaacgcctgg
      661 gggccgcgga tgtggacaag tgggcgctgt atgccatcgg gcagtactgc gaccagacgg
      721 tcccggatgg tttcgggggc acagagccgc ggatgacctt taatgcgtac ctgtcacagc
      781 agcgtaaggt gtgggatgtc ctgggggatt tctgctcggc gatgcgctgt atgccggtat
      841 ggaacggcca gacgctgacg ttcgttcagg accg
//
LOCUS       DAZFQD010000361          874 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.368, whole genome shotgun sequence.
ACCESSION   DAZFQD010000361 DAZFQD010000000
VERSION     DAZFQD010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..874
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.368"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(30..872)
                     /locus_tag="ACHKTW_005361"
     CDS             complement(30..872)
                     /locus_tag="ACHKTW_005361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001351856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HFO6466190.1"
                     /translation="MCSFVAAQPTIVPQLQQQVTDLTSSLNSQEKKELTHKLDSIFNN
                     TQVQIAVLIVPTTKDETIEQYATRVFDNWRLGDAKRNDGILIIVAWSDRTVRIQVGYG
                     LEEKVTDALAGDIIRSNMIPAFKQQKFAQGLELAINALNNQLTSQHQYPTNPSESESA
                     SSSDHYYFAIFWVFAVMFFPFWFFHQGSNFCRACKSGVYISAIYLLDLFLFSDKIFSI
                     AVFSFFFTFTIFMVFTCLCVLQKRASGKSYHSDNSGSGGGSSGGGFSGGGGSSGGGGA
                     SGRW"
BASE COUNT          267 a          179 c          183 g          245 t
ORIGIN      
        1 cctccgggct ggcatttttt atggttgagt taccagcggc cggatgcgcc gccacctcca
       61 gaagaaccgc caccgccgct aaatccgccg ccacttgagc ctccaccgga accgctgttg
      121 tctgaatgat aacttttacc tgatgctctt ttctgaagta cacataaaca ggtaaagacc
      181 ataaatatgg tgaaagtaaa aaagaaggaa aatacagcaa tggaaaaaat tttatcggag
      241 aacaggaata aatctaaaag atagattgct gaaatataga ctccactttt acatgcgcga
      301 caaaaattac tgccttgatg aaaaaaccag aaagggaaga acatcactgc aaatacccaa
      361 aaaatagcaa agtaataatg atcactggaa gacgctgatt cactttctga aggattagtc
      421 ggatattggt gttgtgaagt gagttggtta ttcaaagcgt ttatagctag ctctaatccc
      481 tgagcaaatt tttgttgttt aaatgcgggt atcatgttgc tacggatgat atctccagcc
      541 agagcatcgg ttaccttttc ttccagccca taacctacct ggatgcggac agtacgatct
      601 gaccaggcaa caatgatcag tatcccatca ttacgtttgg catctcctaa acgccaattg
      661 tcaaaaactc ttgtagcata ttgttcaatt gtctcgtctt tggttgtggg aacaattaat
      721 acagcaattt gcacttgggt attattgaaa atagagtcta acttgtgcgt cagttctttc
      781 ttttcttgtg agtttaagct actcgtaaga tccgtaactt gttgctgtaa ttgtgggaca
      841 atagttggtt gggctgcaac aaaggaacac acga
//
LOCUS       DAZFQD010000362          863 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.369, whole genome shotgun sequence.
ACCESSION   DAZFQD010000362 DAZFQD010000000
VERSION     DAZFQD010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..863
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.369"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(74..745)
                     /gene="queE"
                     /locus_tag="ACHKTW_005362"
     CDS             complement(74..745)
                     /gene="queE"
                     /locus_tag="ACHKTW_005362"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HFO6466191.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVIGRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYDVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHK
                     YLNIA"
BASE COUNT          224 a          221 c          189 g          229 t
ORIGIN      
        1 gaggcgacaa acaatataaa tgagtagaag tctttacagg ttccacccat ttataacgct
       61 tataaatgtt taatcaggca atatttagat atttatgtgt ttgcatcgac aaacgccaat
      121 tacgcgcaat gcaggtttca atgcacaaac gtgtggcatc atccttttgg ctaatcggct
      181 gcagtgcaat gactcgcggt ttatcatcgg tcagcgttgc caatagttca tccagtgctt
      241 caatatcgcg tacgcgcccc actggatgct tgatttcgtt ggctcgctcc agtgcctgtg
      301 acaacacgtc atagccgccg cgcatgttca gctttggcga tacggtaacc caggtattcg
      361 gtgtgcagcg tacttcatga gttccactgg tttcgatctg gcagctaaaa ccgttctttt
      421 cgagcaggtc agtcagtggc agcaaatcat gaatgcaagg ctcaccaccc gtaatcacca
      481 catgccgcgc ggtatatccc tgccgaccaa tgacagccag caaatcttca ctgctcgccg
      541 ccccccactt atcactctct ttggtcttcg ccagaatgct gaaaagggag acttcccgat
      601 cctcaagctt ttcccaggtg tgtttggtgt cgcaccaggc acagccaacc gggcatccct
      661 gtaaacgaat aaaaatggcg gggacgccgg taaagtaacc ctcaccttgc agggtctgga
      721 acatctcgtt aatcgggtac tgcatagcat tctctgtgaa gtggataatt gttaattatt
      781 gcagatcctg ccacaacaat catgtcttat taacattctg ttacaggcag atttaaagaa
      841 aaatgcgaaa catatcgtta ata
//
LOCUS       DAZFQD010000363          856 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.370, whole genome shotgun sequence.
ACCESSION   DAZFQD010000363 DAZFQD010000000
VERSION     DAZFQD010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..856
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.370"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(302..>856)
                     /locus_tag="ACHKTW_005363"
     CDS             complement(302..>856)
                     /locus_tag="ACHKTW_005363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO6466192.1"
                     /translation="YYPIDLIEKFQNLYAITDQIRNTTGFCLPQNPELIYCWEQLKTS
                     ISRGFSTQIQEHLAMGVLLSLGAHHVNCLLLSDSKQSLISRCYNLMLSEPGTKWTANK
                     VARYLYISVSTLHRRLASEGVSFQSILDDVRLNNALSAIQTTVKPISEIARENGYKCP
                     SRFTERFHNRFKITPRELRKASRE"
BASE COUNT          251 a          176 c          156 g          273 t
ORIGIN      
        1 aataacaacg atatccgtac ctatccggat atctaatgtc ataagcatcc ctttaattat
       61 cccctcaacc tcttgtaact ttcccactct tgaactccac atagtccaaa cgccggatgc
      121 aaaaaaatca cacgtgcatt tctgaaattg tgatggagga cgatagtatc aattatatgt
      181 gcttataaac gtccccgatt agcgcagaag tcaccggatt gttcaggctc caataggttg
      241 agtatgccgt atttattttc caaaataaaa atcgatgctg ttgctccttc ataaaatact
      301 tttactctct ggacgctttt cttagctctc ttggtgttat cttaaaacga ttatggaacc
      361 tttcagtaaa acgagaagga cacttgtaac cattttccct ggcaatctcg ctgatgggtt
      421 ttactgtcgt ttgtatagca gacaacgcat tatttaacct cacgtcgtcc aatatacttt
      481 gaaaacttac tccctcgctt gccagacggc gatgcaatgt ggaaacagaa atgtagagat
      541 atctcgctac cttgtttgct gtccattttg ttccaggttc ggatagcatt aggttataac
      601 aacgacttat taatgattgt ttactatctg ataaaagtaa acaattaaca tgatgtgctc
      661 ctaatgaaag tagaacgccc attgctaagt gttcctgaat ttgcgttgag aagcctcggg
      721 aaatagatgt ttttagttgc tcccaacaat atattaactc gggattctga ggtaagcaga
      781 aacctgtcgt gttacgaatt tgatcggtta tcgcataaag attttggaac ttttcaatta
      841 gatcaatggg ataata
//
LOCUS       DAZFQD010000364          855 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.371, whole genome shotgun sequence.
ACCESSION   DAZFQD010000364 DAZFQD010000000
VERSION     DAZFQD010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..855
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.371"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>855)
                     /locus_tag="ACHKTW_005364"
     CDS             complement(<1..>855)
                     /locus_tag="ACHKTW_005364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein Rhs"
                     /protein_id="HFO6466193.1"
                     /translation="TTQGRRAIEYHYPDEHTVIRCILPPEDERDRHPGESLLKTTYRY
                     NAAGELTEVILPGDETLTFSRDEAGREVLRHSNRGFACEQGWNAAGQPVSQRAGFFPE
                     EATWGGLVPSLAREYRYDSAGNVSGVTSREDYGRETRREYRLDRNGQVTAVTASGTGL
                     GYGEGDETYGYDSCGYLKAQSAGRHRISEETDQYAGGHRLKQAGNTQYDYDAAGRMVS
                     RTKHRDGYRPETERFRWDSRDQLTGYCSAQGELWEYRHDASGRRTEKRCDRKKIRFTY
                     LWDGDSIAE"
BASE COUNT          120 a          288 c          245 g          202 t
ORIGIN      
        1 ttccgcaata ctgtcgccgt cccacaggta cgtgaagcgg attttcttcc ggtcgcagcg
       61 tttctctgtt cgtctgccgc tggcgtcgtg gcggtattcc cacagctccc cctgtgcgct
      121 gcaatacccg gtcagctggt cccggctgtc ccaccggaac cgctctgttt ccgggcggta
      181 gccgtcacgg tgttttgtcc ggctgaccat ccggcctgcg gcgtcatagt catactgcgt
      241 gtttccggcc tgtttcagcc ggtggcctcc ggcatactgg tcagtctctt cacttatccg
      301 gtgcctgccc gcagactgcg ccttcaggta gccgcagctg tcatagccat aagtctcgtc
      361 gccttcgcca tagcccagcc cggtgcctga ggctgtcacc gccgtgacct ggccgttccg
      421 gtccagccgg tattcccgcc gggtttcccg tccgtaatct tcccggctgg tgacgcctga
      481 cacattaccc gcgctgtcgt aacggtactc ccgcgccagt gacggaacca gcccgcccca
      541 tgtggcttct tccgggaaaa aaccggcgcg ctgactgaca ggctgaccgg ctgcattcca
      601 gccctgttca caggcaaaac cccggttact gtgccggagc acttcacgtc ctgcctcatc
      661 acggctgaac gtcagcgtct catcccccgg caggataacc tccgtcagtt ctccggcggc
      721 gttgtagcgg tacgtggttt tcagcaggga ttcaccgggg tgtctgtcgc gttcatcttc
      781 cggtggcagg atacagcgga tgacggtgtg ttcatcgggg tagtggtatt caatggcccg
      841 tcggccctga gtggt
//
LOCUS       DAZFQD010000365          844 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.372, whole genome shotgun sequence.
ACCESSION   DAZFQD010000365 DAZFQD010000000
VERSION     DAZFQD010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..844
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.372"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..459
                     /locus_tag="ACHKTW_005365"
     CDS             <1..459
                     /locus_tag="ACHKTW_005365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFO6466194.1"
                     /translation="GDISLRFKIPSDAGIAENRYGVGIYWAVSNWPDGDGKNNLIASC
                     FLQTDSANLNVMYHKGSGSQQIGSYGVFDHEWHTLSLRFAGGNSMSVTPVLDGKDGEP
                     FELVKWVNAGFTVDQLTLTDITGNAATYPMLFDTIKVQVNDAVSSSNVNK"
     gene            495..674
                     /locus_tag="ACHKTW_005366"
     CDS             495..674
                     /locus_tag="ACHKTW_005366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HFO6466195.1"
                     /translation="MPILHTFMLYFCTCVCLLYLLSGGYKIIRNYFRNKIDAAAEEKI
                     KSGAVMPQPKDSGQI"
     gene            710..>844
                     /locus_tag="ACHKTW_005367"
     CDS             710..>844
                     /locus_tag="ACHKTW_005367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HFO6466196.1"
                     /translation="MPEIKGTVTEELVKQALYSEEVNRVLKAQVRKDFEAQIDAYVDE
                     V"
BASE COUNT          243 a          152 c          215 g          234 t
ORIGIN      
        1 ggtgatattt ccctccgttt caaaattcca tcggatgcgg gcattgcgga aaaccggtat
       61 ggtgttggta tttactgggc tgtaagtaac tggcctgacg gtgacggaaa aaataacctc
      121 atcgcatcat gtttcctgca aacggattca gctaacctga atgtgatgta tcacaaaggg
      181 agcggaagtc aacagatagg atcgtatggt gtctttgatc atgaatggca tacgctctcg
      241 ttacggtttg caggtggtaa tagcatgagt gttaccccag ttctggacgg gaaagatggt
      301 gaaccatttg aacttgtgaa gtgggtgaat gccgggttca cggtggatca actgacactg
      361 accgatatta ccggcaatgc agccacttac ccgatgttgt ttgacacaat taaagtgcag
      421 gttaacgatg cggtctcttc gtctaatgtg aataaatgac gggtaattca ttttatttaa
      481 aatggtgagt gattatgcca atactacata catttatgct ttatttttgc acgtgtgtgt
      541 gtctgcttta tctcctcagc ggaggttaca aaattatccg aaattatttt cgaaataaaa
      601 ttgatgcagc cgcagaggaa aagataaaaa gcggtgctgt aatgccgcag ccgaaagact
      661 ccggtcagat ttaatttttt gtgaaccaga aaaggaaagg agaatattca tgccagagat
      721 taaaggcacg gttactgaag agcttgtcaa acaggcactt tattctgaag aagtgaaccg
      781 cgtgctgaag gcgcaggttc gcaaggattt tgaggcacaa atcgacgcat atgttgatga
      841 agtg
//
LOCUS       DAZFQD010000366          828 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.373, whole genome shotgun sequence.
ACCESSION   DAZFQD010000366 DAZFQD010000000
VERSION     DAZFQD010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..828
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.373"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
BASE COUNT          253 a          138 c          181 g          256 t
ORIGIN      
        1 aatgtaggaa gtggaattgt attgaggctt agtgaaatta cacgaggaaa gcagagtgta
       61 attgagcttt agccgctctc tcttcgagta attacacaac tacagagaat aacagagtta
      121 ttatatgtag gaagtggaat taagaggtag agagagagag ttaacacata agcaatctgt
      181 agattggtta ggtgattact cttactgcgc acagcgcaat agtatttctt cgtgggcgtt
      241 accgccaacg atagttacac gaagaaagta acgctttgct ttcggtgtga gcggaacggg
      301 ccagccaacg tagcccgaaa ggtagcggag taaggctgct tgccagagag tgaagctaac
      361 gccggaaggc aaaagatcat atatatatag atataaagaa tattatttaa ttattattta
      421 attattctta ttaacttatt cttatcgggt atcagttttg ataggggtag gggtatcaat
      481 attgataggg gtagtcatat caaagttaat aggggtatca atattgatag gggtatcatc
      541 ggtttattat tctttataaa cccctgttag acttgatagg ggtaacagaa ttctgtgggg
      601 gtagttcact gtgagctacc gttacatcca tttgtcacat agcactcact ctgtgtaatg
      661 cctctctatg tgcgtgccat aatcccccta tattccctgt gtaaatcata gaatatccct
      721 aacttattcc tgtgtaattc catactaaaa attcaatgaa aagtagctta aaagtgcaat
      781 tcttactttc ttcactttta catcactttc gcaaagcctt gtgtaatg
//
LOCUS       DAZFQD010000367          825 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.374, whole genome shotgun sequence.
ACCESSION   DAZFQD010000367 DAZFQD010000000
VERSION     DAZFQD010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..825
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.374"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            127..>825
                     /locus_tag="ACHKTW_005368"
     CDS             127..>825
                     /locus_tag="ACHKTW_005368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFO6466197.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGSDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPTDLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQKVYH"
BASE COUNT          272 a          138 c          175 g          240 t
ORIGIN      
        1 attatggctt tagaatattt ttatttctaa tagacgagat ttttcctgtt atgatataat
       61 atgccgaatt aacacatgtt aacgatttac cagtaatgta aataaatttt cgaggagatc
      121 attccagtgg gacgtaaatg ggccaatatt gttgctaaaa aaacggctaa agacggtgca
      181 acgtctaaaa tttatgcaaa attcggtgta gaaatctatg ctgctgctaa acaaggtgaa
      241 cccgatccag aattaaacac atctttaaaa ttcgttattg aacgtgcaaa gcaggcacaa
      301 gttccaaagc acgttattga taaagcaatt gataaagcca aaggcggcgg agatgaaacg
      361 ttcgtgcagg gacgttatga aggctttggt cctaatggct caatgattat cgccgagaca
      421 ttgacttcaa atgttaaccg tacgattgct aacgttcgca caattttcaa taaaaaaggc
      481 ggcaatatcg gagcggcagg ttctgtcagc tatatgtttg acaatacggg tgtgattgta
      541 tttaaaggat cagaccctga ccatattttt gaaattttac ttgaagctga agttgatgtt
      601 cgtgatgtga ctgaagaaga aggtaacatt gttatttata ccgaacctac tgaccttcat
      661 aaaggaatcg cggctctaaa agcagctgga atcactgagt tctcaacaac agaattagaa
      721 atgattgctc aatctgaagt tgagctttcc ccagaagatt tagaaatctt tgaagggctt
      781 gttgatgccc ttgaagatga cgacgatgta caaaaagttt atcat
//
LOCUS       DAZFQD010000368          809 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.375, whole genome shotgun sequence.
ACCESSION   DAZFQD010000368 DAZFQD010000000
VERSION     DAZFQD010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..809
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.375"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(1..670)
                     /locus_tag="ACHKTW_005369"
                     /pseudo
     CDS             complement(1..670)
                     /locus_tag="ACHKTW_005369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
BASE COUNT          234 a          168 c          136 g          271 t
ORIGIN      
        1 cgtaactgtc atgctctgga ttatttaatt ctttcagcgg aaatttttcc tcaaaggctt
       61 tatttagccg cccctggttt ccttttaccg cgaataaata atcacctccc tgtttttgta
      121 tcttttctgc aatatctttc tggcagccca tcgcatcagt tgtgatgatt tttcctttaa
      181 tatccatcat gttaagaagt tcaggaatgg ctgtaatctc attggatttc tcatccgtct
      241 tgatctgtcc gaggaccaga ctatgcattg ttgagaacgc actaatgaca tgaatcgctc
      301 ccctgcgacg actcttgtca taagagtgcc ggagcgtttt ttcatcaatt gcaatgacgt
      361 ctttatcatc tgaagaatgg cagtcacgca tccagttaat aaagcactcg tgaaattttg
      421 caggactgat acaggataca actctggcaa tgatatcgtg aacaggaata ccattttcaa
      481 aatcaccata tcacttcaaa acatcgggat gtgtttcccc aaaatcctct atatcttccc
      541 agccttctgc accagaaata acggcacaaa tagtcaacag tagaatatcc gataatttat
      601 gttccacttt ccaggcttgt ctgtaatcgg ggataataga aatatgttcc atcaattttt
      661 taagttccat tttgttctcc ttaattatgt aaggagtatt tgatcatgta taagcaataa
      721 aaaacagctt caggtaatga ggaatatctc aatttttaaa cataaaatgt gaattattta
      781 gtacaaaaag tgtacttctg atgatcttt
//
LOCUS       DAZFQD010000369          807 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.376, whole genome shotgun sequence.
ACCESSION   DAZFQD010000369 DAZFQD010000000
VERSION     DAZFQD010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..807
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.376"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..325
                     /locus_tag="ACHKTW_005370"
     CDS             <1..325
                     /locus_tag="ACHKTW_005370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001039288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6466198.1"
                     /translation="YDTIYSLINYYEDDYLLPLNRAELEAYKENTPSALNEAFKHWDL
                     AVNAFEHLSKRVEMLCKRENAYLTADQIWELSNWIEDIESDVRYVGDGLVELAQRLGA
                     TITEE"
BASE COUNT          267 a          135 c          151 g          254 t
ORIGIN      
        1 gtatgacaca atttactcac taattaatta ttatgaggat gattatctcc tgcctttaaa
       61 ccgcgctgaa ctcgaagcat acaaagaaaa caccccatcg gcactaaatg aggcgtttaa
      121 acactgggat ttagctgtga atgcctttga gcacctctct aagcgtgtag agatgctctg
      181 taagcgcgaa aacgcatatc taacagcgga tcagatatgg gagttatcaa actggataga
      241 agatattgaa tctgatgttc gctatgtagg agacggcctt gttgaactag ctcaacgctt
      301 aggcgctact atcacagaag aataaaaaaa gaaacaaaaa agttgtaaaa tatcttgaca
      361 ttccatctat tgtatgttaa tataattata tggggtgatt atttttcctt tattggttcg
      421 atattaaaaa ttcttcttgt cgttaaaggg gagtaaaatc tccgctcccc gcttctcctc
      481 ctccttaagt gtcattatta cctctttttt cctttgttat gcatacatct tggagtatgc
      541 ataacatagg gtaattacat aggtacaagt taataaaagg cggctctaaa tgctgccttt
      601 tattttgccc gttatgcaac ttaaaagttg tatttcttac tttaaacaag gtaacaaggt
      661 ttatataaat gttgttttta aatgataaag agcaaatcat caaatataaa gatgaaatgc
      721 tgaaaataaa tccacaaatt accgaaatgg tagctaaata cgctgggtgt tctgtagagg
      781 aagtggaaag atgtgttgaa aaatatt
//
LOCUS       DAZFQD010000370          794 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.377, whole genome shotgun sequence.
ACCESSION   DAZFQD010000370 DAZFQD010000000
VERSION     DAZFQD010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..794
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.377"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            43..564
                     /locus_tag="ACHKTW_005371"
     CDS             43..564
                     /locus_tag="ACHKTW_005371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFO6466199.1"
                     /translation="MQLINVKRYYPENTLYGNDVQYFQSEDGKDFYESLPLFTKKYKL
                     CITPDSGIICSISQDASALYPAGFSVVEVDELPEGADISGNWKFDNGVISRIPVNYAR
                     KLEAMRQLYLNQAYEKINDWRTELQLGTISDEDRAALTRWMAYISQVKKMEPSAIKTE
                     AEFDAIKWPEQPE"
     gene            complement(599..>794)
                     /locus_tag="ACHKTW_005372"
     CDS             complement(599..>794)
                     /locus_tag="ACHKTW_005372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO6466200.1"
                     /translation="LGAQSYHSPGGNEMSWNYSAPSGCMLSGINVQETGKNSADNIGG
                     VYFRPVQIYISNAWRTVSSV"
BASE COUNT          236 a          171 c          168 g          219 t
ORIGIN      
        1 gtattattcc tgtttttatc attgattcac atggtgatgt ttatgcaact gattaatgtt
       61 aaacgatatt acccggaaaa caccctctat ggtaatgatg tacagtattt tcagagtgaa
      121 gatggtaagg atttctacga gtccctgcca ttattcacca aaaaatataa actttgtata
      181 acacctgaca gcggcattat ctgctcaata tcacaggacg cttcggcgct gtatcctgcg
      241 gggttctctg tggttgaagt cgacgaactc cctgaaggtg ctgatatttc gggtaactgg
      301 aagtttgata atggcgtgat ctcccgtatt ccggttaact acgcaagaaa actggaagca
      361 atgcgccagt tatatcttaa tcaggcatat gaaaaaatta atgactggag aacagaatta
      421 cagctgggca ccatcagtga tgaagacaga gccgcactta cccgatggat ggcatacatc
      481 agtcaggtga aaaaaatgga accatccgcc attaaaactg aagctgaatt tgatgccatt
      541 aaatggcccg aacagcctga ataacttaaa gggtgcttat cgcacccttt ttctttcatc
      601 agacagatga aactgtacgc catgcattac tgatatatat ctgcacagga cggaaataaa
      661 ccccgccaat gttatcggca gagttttttc ctgtttcctg cacgttaata ccggaaagca
      721 tacaaccgga aggcgcactg taattccatg acatttcatt gcctccgggt gaatgatagc
      781 tctgtgctcc aagc
//
LOCUS       DAZFQD010000371          766 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.378, whole genome shotgun sequence.
ACCESSION   DAZFQD010000371 DAZFQD010000000
VERSION     DAZFQD010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..766
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.378"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            8..283
                     /gene="yddL"
                     /locus_tag="ACHKTW_005373"
                     /pseudo
     CDS             8..283
                     /gene="yddL"
                     /locus_tag="ACHKTW_005373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310103.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
     gene            322..>766
                     /locus_tag="ACHKTW_005374"
     CDS             322..>766
                     /locus_tag="ACHKTW_005374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFO6466201.1"
                     /translation="MITDLILHNHPRMKTITLNDNHIAHLNAKNTTKLEYLNLSNNNL
                     LPTNDIDQLISSKHLWHVLVNVINNDPLAQMQYWTAVRNIIDDTNEVTIDLSGLNLTT
                     QPPGLQNFTSINLDNNQLTHFNATNYDRLVKLSLNSNTLESINFPQ"
BASE COUNT          272 a          156 c          128 g          210 t
ORIGIN      
        1 ctgttaaatg aaattaaaaa tagttgcggt ggttgtaact ggtttgttag ctgcgaacgt
       61 agcacacgct gccgaagtct ataacaagga tggtaataaa ctcgaccttt atggcaaggt
      121 taccgctcta cgttatttta ctgatgataa gcgtgacgat ggtgataaaa cttatgcccg
      181 tctcggcttt aaaggagaaa cgcaaatcaa tgatcaaatg attggttttg gtcactggga
      241 atatgatttt aaaggctata acgatgaagc caacggctcg cgcggcaaga atctctgatt
      301 gagttaagtg cccatcacaa tatgatcact gaccttatat tacacaatca tcccaggatg
      361 aaaacaatca ctttaaacga caaccatatt gcacatttaa acgccaaaaa cactacaaaa
      421 ctggaatatt taaacttaag caataacaat ttactgccaa ccaatgacat tgatcaacta
      481 atatcatcaa aacatctttg gcatgtatta gttaacgtca tcaacaatga tccacttgca
      541 caaatgcagt actggactgc agtaagaaat ataattgatg acactaatga agtgaccatt
      601 gatttatcag gacttaattt aaccactcaa ccaccagggc tgcaaaactt cacctctatc
      661 aatcttgata ataaccaact cacacatttt aatgcaacca actacgatag actcgtaaag
      721 ctaagtctga atagtaatac tcttgagtca ataaattttc ctcaag
//
LOCUS       DAZFQD010000372          749 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.379, whole genome shotgun sequence.
ACCESSION   DAZFQD010000372 DAZFQD010000000
VERSION     DAZFQD010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..749
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.379"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     repeat_region   208..663
                     /inference="COORDINATES: alignment:crt:1.2"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=208..236
                     /rpt_unit_seq="gagttccccgcgccagcggggataaaccg"
BASE COUNT          189 a          172 c          190 g          198 t
ORIGIN      
        1 tggcattaat ttttcgctgg agtgaaaatt attgcggtaa agtttggtag attttaattt
       61 gtatagagtt attttaaata tttacctttt taatcaatga attaagtact ctttaacata
      121 atggatgtgt tgtttgtgtg atactgtaaa gttggtagat tgtgactggc ttaaaaaatc
      181 attaattaat aataggttat gtttacagag ttccccgcgc cagcggggat aaaccgccgc
      241 ttaaccggac ctcatcacaa aacacgcgga gttccccgcg ccagcgggga taaaccggct
      301 atcgggtgtg gataccgctt tcgaggcgtg agttccccgc gccagcgggg ataaaccgga
      361 ctcgggtggc aagtattccc cgaatgctgg gagttccccg cgccagcggg gataaaccgc
      421 ctgttgcgcc ttataatgtg tataccgcga agagttcccc gcgccagcgg ggataaaccg
      481 acgctgattt gatgggggct ggtgcaggag atgagttccc cgcgccagcg gggataaacc
      541 gtttcagcag ttcagcgtaa caccgacggt cacgagttcc ccgcgccagc ggggataaac
      601 cgcccggaat gcattctgaa ggtttgctgt atatgagttc cccgcgccag cggggataaa
      661 ccaccatata acccgttatc tctttctcaa gtttttatat tagcagtact tgtaataagc
      721 aacatatcca cgtaacacct catgttcaa
//
LOCUS       DAZFQD010000373          742 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.380, whole genome shotgun sequence.
ACCESSION   DAZFQD010000373 DAZFQD010000000
VERSION     DAZFQD010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..742
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.380"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            282..>742
                     /locus_tag="ACHKTW_005375"
     CDS             282..>742
                     /locus_tag="ACHKTW_005375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HFO6466202.1"
                     /translation="MSKELLSGTPLKWVGGKSRIMNTLKAHLPEADCLVEPFVGGASV
                     FMNTSYKRYILGDSNGALINFYRQARDNTDSLIDEAKTLFLNHNNRGDYLIIRETFNA
                     ENHAFLNSRTGISDRRELRLAAMFLYLNRHSFNGLFRVNGKGDFNVPFGCYR"
BASE COUNT          197 a          194 c          190 g          161 t
ORIGIN      
        1 gtgtcagaaa tctggcagcg cttcacaaca ccattcctga acatatgagg tgaagccatg
       61 gtcgccatcc tgaatgtgtg ttgctgcccc cccctgaacg tggaaagaaa agcccgcgca
      121 tcgctgcgtt tattccgcga cggcgtgcgg aatttttcac gcataaaacc ccacgactac
      181 ctggtaataa gaatcggttt tcgctggcga ttgctgagca aaaacaacgg ggcgtcatgg
      241 gatttattaa cgcacgaaac ctataacaaa gaggccgcgt tatgagcaaa gaactgttat
      301 ccggcacgcc gcttaaatgg gtgggcggaa aaagtcgcat catgaacaca ctgaaggcgc
      361 atctgccaga agcagactgc ctggttgagc cgtttgtggg tggcgcatcg gtattcatga
      421 acaccagtta caagcgttac atcctgggtg attccaatgg tgcgctgatt aatttctacc
      481 ggcaagccag ggacaacacc gacagcctga tcgacgaggc gaaaaccctg ttcctgaacc
      541 acaacaacag gggtgactat ctcatcattc gcgaaacctt taacgcagag aatcatgcct
      601 tcctgaacag cagaacggga ataagcgacc gccgtgaact gcgactggcc gccatgtttt
      661 tgtacctgaa ccgacacagc tttaacggtt tgttccgcgt gaacggaaaa ggcgatttta
      721 acgtgccttt cggctgctac cg
//
LOCUS       DAZFQD010000374          721 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.381, whole genome shotgun sequence.
ACCESSION   DAZFQD010000374 DAZFQD010000000
VERSION     DAZFQD010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..721
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.381"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..528
                     /locus_tag="ACHKTW_005376"
     CDS             <1..528
                     /locus_tag="ACHKTW_005376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6466203.1"
                     /translation="DAKVLEGVKNWLSIYGRLTCGILAEKMNMPPSSMVYFLRDAVDA
                     GVLTECNGFYDIPRPRPVQPVRRKCSQEGAADDVQWCSFRKSLPWIEGHDIPSMAWEF
                     AQGVLTCETVYVVAEVDEQAMKEGVPQFVMAYIDIRLGVIICGLSGWNITEHVLRYLI
                     VDRTAAPAAISAEVE"
     gene            528..>721
                     /locus_tag="ACHKTW_005377"
     CDS             528..>721
                     /locus_tag="ACHKTW_005377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6466204.1"
                     /translation="MFFKTSNPAALAAWQKYQQDCQTVKDEAKRLEAVLNVACRSVFE
                     FSISGFCFKGLRFMDDKYPF"
BASE COUNT          155 a          154 c          219 g          193 t
ORIGIN      
        1 gatgcaaaag tgcttgaagg ggttaaaaac tggctgagta tttacgggcg tctgacctgc
       61 ggcattctgg ctgaaaaaat gaatatgccg ccatcctcga tggtttattt tctgcgtgat
      121 gcggtcgatg ccggggtgct gacggaatgt aacggttttt atgatattcc gcgtccccgc
      181 ccggtgcagc cggttcgtcg caaatgcagc caggaaggtg cggctgatga tgttcagtgg
      241 tgcagcttca gaaaatccct gccgtggatt gaggggcatg atattccgtc gatggcgtgg
      301 gaatttgctc agggcgttct gacctgtgaa accgtttatg tggtggctga agttgatgag
      361 caggccatga aagaaggcgt gccccagttt gtgatggcgt atatcgacat tcgcctgggc
      421 gtcattatct gcggtttaag cggctggaat atcaccgaac atgttctgcg ttacctgatt
      481 gttgaccgga cggctgcgcc tgccgcgatt tctgcggagg tagagtaatg ttctttaaaa
      541 catcaaaccc tgccgcgctg gctgcgtggc aaaaatacca gcaggactgc cagacggtaa
      601 aagatgaggc gaaacgcctt gaggccgtgc tgaatgttgc gtgccggtcg gtatttgaat
      661 tcagtatcag tggcttttgt tttaaggggc tgcgctttat ggatgacaaa tatccttttc
      721 a
//
LOCUS       DAZFQD010000375          718 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.382, whole genome shotgun sequence.
ACCESSION   DAZFQD010000375 DAZFQD010000000
VERSION     DAZFQD010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..718
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.382"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(170..391)
                     /locus_tag="ACHKTW_005378"
     CDS             complement(170..391)
                     /locus_tag="ACHKTW_005378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000013462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="derepression protein"
                     /protein_id="HFO6466205.1"
                     /translation="MANRKQQRARAERRHIQTEINRRLFRATRVAQIMHINMLHERTH
                     ALSNRYCAAVFSYLAEDLAELQSLINQHR"
     gene            452..640
                     /locus_tag="ACHKTW_005379"
     CDS             452..640
                     /locus_tag="ACHKTW_005379"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6466206.1"
                     /translation="MARRTGFPQPANEETGPTEVGPVRPAIILTRKRRGDTVRTKKPL
                     ARLCASFVSGVEIPAPVL"
BASE COUNT          158 a          174 c          223 g          163 t
ORIGIN      
        1 tagtcgtgcc agttgttcag gcgggaagcc agcaggggat ccaccgccca catccagtta
       61 aaaaagtcct gaatatctga aagtccctta acgctcttca tgagaacctc cggtaacaga
      121 cgtgcggaag cctcccgcgc acgggtgcgg gatggcttca ggatgtggtt tatcggtgct
      181 ggttgatgag gctttgcagt tctgccagat cttcggcgag atagctgaag acagcagcgc
      241 agtaacggtt tgatagtgcg tgcgtgcgct catgcagcat gttgatatgc atgatttgcg
      301 cgacgcgtgt tgcgcggaaa agtctgcggt tgatttcagt ctggatgtga cgacgctcag
      361 cgcgggcgcg ctgttgcttg cggtttgcca tggtgtggcc tcttcgtttg tgaattttga
      421 aaactcacca accagaggtg gaaaactcag ggtggcgaga cgtacagggt ttccacaacc
      481 ggcaaacgaa gaaaccggcc cgaccgaagt cggccctgta cgccccgcca taattctgac
      541 gcgaaaaaga cgtggcgata cagtacgcac aaaaaaaccg ctggcgcggc tgtgcgcttc
      601 gtttgtcagc ggggtggaaa tcccggcacc cgttttatga ggtgcagcgg aaatgtaacc
      661 cgacacgacg agcgctggca agcggttttt gtgttgcgtg ctgatgtggt tagaatgt
//
LOCUS       DAZFQD010000376          717 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.383, whole genome shotgun sequence.
ACCESSION   DAZFQD010000376 DAZFQD010000000
VERSION     DAZFQD010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..717
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.383"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(1..443)
                     /locus_tag="ACHKTW_005380"
                     /pseudo
     CDS             complement(1..443)
                     /locus_tag="ACHKTW_005380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308632.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein Rhs"
     gene            complement(516..>717)
                     /locus_tag="ACHKTW_005381"
     CDS             complement(516..>717)
                     /locus_tag="ACHKTW_005381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6466207.1"
                     /translation="YDSIPISEIDVFNEYCFYSKAKKQYENERWESCKAFEPCSDLVL
                     SSSLTIAIKIYMRLNPKMKILD"
BASE COUNT          171 a          195 c          165 g          186 t
ORIGIN      
        1 tcatagccgg tgcgctgtga tgtaccgtcc gggctgaaga tacccgtcag cctgccggat
       61 ttgtcatacg catactgcgt tttgccttcc ggcgtgatga tttctgccag gcggcctgcg
      121 gcgtcccggc tgtagaggat agtttcctcc aggccgttaa ccctggcggt acacaggcca
      181 tcggcgtcat agcggtaacg ccagaccacg ccgccccagt catgctcttc cgtcacccgg
      241 ccacagttat ccagaacata aaggtatgat tcgccctgcg ggttaatgac ttctgtcagt
      301 tgcagcgtgt ctttatcgta gcggtactgc cagcggtggc cttcaccgtc cgttcgcgcc
      361 accggcaaat cgaacgggcc gtattccatc gtccagacaa caccgttgcc gtccgtccag
      421 gccagcaggt tgccgtggcg gttgcagggt cacggaaggg tgcacctgca acacccggtt
      481 cgcaataact caatatagag tctgaacgac acagattaat ccaatatttt cattttagga
      541 tttaaacgca tataaatctt tatcgctatt gtcaaagaac tcgacaaaac taaatcagaa
      601 catggttcga atgctttaca agactcccat cgttcatttt catattgttt ctttgctttt
      661 gagtaaaaac aatactcatt gaaaacatca atttctgata taggaatact atcataa
//
LOCUS       DAZFQD010000377          712 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.384, whole genome shotgun sequence.
ACCESSION   DAZFQD010000377 DAZFQD010000000
VERSION     DAZFQD010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..712
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.384"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>712
                     /locus_tag="ACHKTW_005382"
     CDS             <1..>712
                     /locus_tag="ACHKTW_005382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HFO6466208.1"
                     /translation="LTAVKDTQGHETRYEYNAAGDLTAVIAPDGSRNGTQYDAWGKAI
                     CTTQGGLTRSMEYDAAGRVIRLTSENGSHTTFRYDVLDRLIQETGFDGRTQRYHHDLT
                     GKLIRSEDEGLVTHWHYDEADRLTHRTVNGETAEQWQYDERGWLTDISHISKGHRVTV
                     HYGYDEKGRLTGERQTVHHPQTEALLWQHETRHAYNAQGLANRCIPDSLPAVEWLTYG
                     SGYLAGMKLGDTPLVEYTR"
BASE COUNT          177 a          213 c          221 g          101 t
ORIGIN      
        1 gttaactgcg gtgaaagaca cgcagggcca tgaaacgcgg tatgaataca acgccgccgg
       61 tgacctgacc gccgtcattg ccccggacgg cagcagaaac gggacacagt acgatgcgtg
      121 gggaaaagcc atctgtacca cgcagggcgg tctgacgcgc agtatggaat acgatgctgc
      181 cggacgggtc atccgcctga ccagtgaaaa cggcagccac accaccttcc gttacgatgt
      241 actcgaccgg ctgatacagg aaaccggctt tgacggccgc acacagcgtt atcaccacga
      301 cctgaccggc aaacttatcc gcagcgagga tgaggggctg gtcacccact ggcactatga
      361 cgaagcagac cgcctcacgc accgcaccgt gaatggcgaa accgcagagc agtggcagta
      421 tgacgaacgc ggctggctga cagacatcag ccatatcagc aaagggcacc gggtgacggt
      481 gcattacggg tatgatgaga aaggccggct gaccggtgag cgtcagacgg tgcatcaccc
      541 gcagacggaa gcactgctct ggcagcatga gaccagacat gcgtacaacg cgcaggggct
      601 ggcgaaccgc tgtataccgg acagcctgcc cgccgtggaa tggctgacct acggcagcgg
      661 ttacctggca ggcatgaaac tcggcgacac accgctggtg gagtacaccc gc
//
LOCUS       DAZFQD010000378          693 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.385, whole genome shotgun sequence.
ACCESSION   DAZFQD010000378 DAZFQD010000000
VERSION     DAZFQD010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..693
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.385"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>693
                     /locus_tag="ACHKTW_005383"
     CDS             <1..>693
                     /locus_tag="ACHKTW_005383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element ISCfr6 family transposase"
                     /protein_id="HFO6466209.1"
                     /translation="SAGARTLAEMLTQNGVPMSRYRAGRLMKYLNLSSCQPGKHQYKN
                     ARQEHTSLPNLLERQFAVPEPDRVWCGDITYLWAGNRWCYLAVVMDLFARRVIGWSLS
                     AHADTALISSALRMAYETRGQPRDVVFHSDQGSQYTGLKYQQLLWRCRINQSVSRRGN
                     CWDNSPMERFFRSLKTEWVPTNGYAGKDEARQQINDYILNYYNSVRPHHYNGGLTPEE
                     SENRYHFYCKTVA"
BASE COUNT          178 a          175 c          193 g          147 t
ORIGIN      
        1 ctctgcgggc gcgcgcactc tggctgaaat gctgactcaa aacggcgtcc cgatgagccg
       61 ttaccgtgcc ggacgtctga tgaaatatct gaacctgagc agttgtcagc ccggaaaaca
      121 tcagtataaa aatgcccgtc aggagcatac cagcctgccg aatctgcttg agcgtcagtt
      181 cgcagtaccg gagccagacc gggtatggtg tggagatatt acgtatctct gggcaggaaa
      241 tcgctggtgc tatctggcgg ttgttatgga tctttttgcc cgcagggtta tcggctggag
      301 tctgtcagcg catgccgata ccgcactgat aagcagtgcc ctgcggatgg cctatgagac
      361 gcgtggccaa ccccgggatg tcgtgttcca tagcgaccag ggaagccagt atacaggcct
      421 taaatatcaa caacttctct ggcgttgcag aataaatcaa agcgtcagtc ggcggggaaa
      481 ctgctgggat aacagcccca tggaacgctt cttccgcagt ctgaaaacag aatgggtgcc
      541 aacgaatggt tacgcaggca aggacgaggc ccggcagcaa attaatgatt acatattgaa
      601 ctactacaac agcgtcagac ctcaccatta taacggtggg ctgacgccgg aagagtcaga
      661 gaacagatac catttttact gtaaaaccgt ggc
//
LOCUS       DAZFQD010000379          682 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.386, whole genome shotgun sequence.
ACCESSION   DAZFQD010000379 DAZFQD010000000
VERSION     DAZFQD010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..682
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.386"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..252
                     /locus_tag="ACHKTW_005384"
     CDS             <1..252
                     /locus_tag="ACHKTW_005384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6466210.1"
                     /translation="VTLIYFLPGEDSFYSAPYEYSRGSSKSCSGAFVDDPDLQKTIFI
                     CYPYGDYQDGNVIYVKKRVNALGAVVTYAYATSGRFRFD"
     gene            complement(302..619)
                     /locus_tag="ACHKTW_005385"
     CDS             complement(302..619)
                     /locus_tag="ACHKTW_005385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFO6466211.1"
                     /translation="MPTQVATEERLAADPNTRRTTKGTNVTTAQLAVSLPCHGAEQPT
                     TMWLYLISFGKQHEELAKHRKGDLILVHGNLQVNRYQDKYGQQKEGWQLLAQSIISSR
                     TGQ"
BASE COUNT          127 a          155 c          177 g          223 t
ORIGIN      
        1 gttacgctga tatattttct tccgggtgaa gattcattct attccgctcc ctatgagtac
       61 agtcgcggta gctcaaaaag ttgttcaggg gcatttgttg atgatcctga tttgcaaaaa
      121 acaatcttta tttgctaccc atacggtgac tatcaggatg gtaatgtgat ttatgtgaag
      181 aagcgtgtga atgcgctggg tgcggtggtc acctatgcat acgcgacaag cggccgattt
      241 cggtttgact gaaaaaagaa atcctgtgtt accctcgctg ctgagggtaa catgcgtgaa
      301 gtcattgccc ggttcttgag ctgataatgc tttgcgccag caactgccag ccctctttct
      361 gttgcccgta cttgtcctga tacctgttta cctgcaagtt tccgtgcacc agaatcagat
      421 cgccttttct gtgttttgcc agttcttcgt gctgcttccc gaatgaaata aggtaaagcc
      481 acattgtggt tggctgttct gcgccatggc agggtagtga taccgccagt tgtgccgttg
      541 tgacgttggt tcctttggtt gttctccttg tgttcgggtc tgctgccagt cgttcctctg
      601 ttgcaacctg tgttggcata tttgtctccc gtgttggttt tattgttggg gttagatgac
      661 tggcagctct tcgccagcgc cg
//
LOCUS       DAZFQD010000380          663 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.387, whole genome shotgun sequence.
ACCESSION   DAZFQD010000380 DAZFQD010000000
VERSION     DAZFQD010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..663
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.387"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            295..474
                     /locus_tag="ACHKTW_005386"
     CDS             295..474
                     /locus_tag="ACHKTW_005386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HFO6466212.1"
                     /translation="MTFIHQVMLYFCTAVCVLYLLSGGYRAVRDFWRRQIDKRAAEKI
                     SASQSAGSKPEEPLI"
     gene            515..>663
                     /locus_tag="ACHKTW_005387"
     CDS             515..>663
                     /locus_tag="ACHKTW_005387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HFO6466213.1"
                     /translation="MSEITSLVTAEAVKEVLRSEEVRSALKQQLRQNLEARLDAEVDA
                     ILDELL"
BASE COUNT          191 a          147 c          176 g          149 t
ORIGIN      
        1 cagagcagac ggcgtttgac cttgtgatgt ggacaaatga tggctttaca gcagatacgc
       61 tgacgctgac agatatcacg ggggcaaaag cgacgtatcc tgtactgctt gatacggtca
      121 cagtcaaagt taacgaaagc cgggcatcat cataaccggc aaaaaaagcc gccagcggca
      181 ggaacggaag ctggcggagg taatcccaat ggagaatgta aagaaaagat gctttcgtat
      241 atcggttttt taaatgaaaa cagttctcat tgtcaaccat aacggtaaga aactatgaca
      301 tttattcatc aggtgatgct gtacttctgt acggcggtct gtgtgctgta tcttctttcg
      361 ggtgggtaca gggcagtgcg cgatttctgg cgcaggcaga ttgataaaag ggccgctgag
      421 aaaatcagcg ccagtcagtc agccggaagc aaacccgaag agccgctcat ttagctgcaa
      481 ctttcttaat cacacctttc gacgagaaaa tcccatgtca gaaattacat ccctggtcac
      541 tgcagaggca gtgaaggaag tcctgcgctc tgaagaagtc agaagcgcac tgaaacagca
      601 acttcggcag aaccttgagg cgcgtcttga tgcagaagtg gatgccattc tggatgaact
      661 gct
//
LOCUS       DAZFQD010000381          649 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.388, whole genome shotgun sequence.
ACCESSION   DAZFQD010000381 DAZFQD010000000
VERSION     DAZFQD010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..649
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.388"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(248..>649)
                     /locus_tag="ACHKTW_005388"
     CDS             complement(248..>649)
                     /locus_tag="ACHKTW_005388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HFO6466214.1"
                     /translation="NMFNRLLVSKKLTKTAVNEVLRRMKKSGLEKPELEAFFRDMTRG
                     KQKSWLSHCTDTEALIIDRVISKVLGEYPGLINILRQRYEGRGMSKRKMAECLNRTHP
                     EWCFSTCEKRIAGWLAVAEHMLYVPMHDSFR"
BASE COUNT          175 a          169 c          112 g          193 t
ORIGIN      
        1 agcatgtctt tttccctttt ccggttaacg tgacacacca ataactcttg tcgaaaaagc
       61 cagcaacctg aaagaacggt attaatgacc accagcgaat ttattgcgct gctgtatatt
      121 acggacacaa aaaaaaccac cttccggtgg cttccttgtg cgaaaaaact tgcatttcgc
      181 ctcgcgatac agctttgcga agcttacagg aattcaagct gtttctgcgt aaaaaagcaa
      241 gcttttttta tcgaaatgaa tcgtgcatag gtacataaag catgtgttca gccacggcta
      301 accaacctgc aatacgtttc tcacatgtgc tgaaacacca ttccgggtga gtacgattta
      361 aacattctgc catttttctc ttactcattc cccgtccttc gtacctttgc cggagaatat
      421 tgattagccc gggatattcc ccaagcacct tactgataac gcgatcaata atcaacgcct
      481 ctgtgtctgt acaatgtgac aaccagctct tctgctttcc tcttgtcata tcccgaaaaa
      541 atgcctcaag ttccggtttt tccagcccgg atttcttcat tctgcgcaaa acctcattaa
      601 ctgctgtttt cgtcagcttt ttcgaaacca gtaaccggtt aaacatatt
//
LOCUS       DAZFQD010000382          647 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.389, whole genome shotgun sequence.
ACCESSION   DAZFQD010000382 DAZFQD010000000
VERSION     DAZFQD010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..647
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.389"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>647)
                     /locus_tag="ACHKTW_005389"
     CDS             complement(<1..>647)
                     /locus_tag="ACHKTW_005389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CHASE9 sensor domain-containing protein"
                     /protein_id="HFO6466215.1"
                     /translation="AALSFIGLFFIINYQLVSERAVKRADSRFELIQKNVGYFFKDIE
                     RSALTLKDSLYLLKNTEEIQRAVILKMEMMPFLDSVGLVLDDNKYYLFSRRANDKIVV
                     YHQEQVNGPLVDESGRVIFADFNPSKRPWSVASDDSNNSWNPAYNCFDRPGKKCISFT
                     LRINGKDHDLLAVDKIHVDLNWRYLNEYLDQISANDEVLFLKQGHEIIAKNQLAR"
BASE COUNT          195 a          142 c          121 g          189 t
ORIGIN      
        1 acgagcgagt tgattcttgg caatgatctc atgaccttgt ttcaaaaata gaacttcatc
       61 attagcgctg atttgatcaa gatactcgtt cagataccgc cagtttaaat cgacatgaat
      121 tttatctacc gctaacaaat cgtgatcttt gccgttgata cgtagcgtaa aagagataca
      181 ttttttaccc ggacgatcaa agcaattgta tgccggattc cagctgttgt tagagtcatc
      241 tgaagccacc gaccacggtc gtttcgatgg gttaaaatcg gcaaaaataa cccgccctga
      301 ctcgtcgaca agcggtccat ttacttgttc ctgatgataa acaacgattt tatcattcgc
      361 cctccgcgaa aagagataat atttattatc atcaagtacc agtcccaccg agtctaaaaa
      421 tggcatcatt tccattttca gaatcacggc gcgttgaatc tcctctgtat tttttaataa
      481 atacagtgag tcctttaatg tcagggccga acgttcaata tctttaaaga aatagccaac
      541 gtttttctga ataagttcaa agcggctatc ggcacgtttt accgcgcgtt ccgataccag
      601 ttgatagtta atgatgaaaa aaagaccaat aaatgacagt gctgcca
//
LOCUS       DAZFQD010000383          633 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.390, whole genome shotgun sequence.
ACCESSION   DAZFQD010000383 DAZFQD010000000
VERSION     DAZFQD010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..633
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.390"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(1..573)
                     /locus_tag="ACHKTW_005390"
                     /pseudo
     CDS             complement(1..573)
                     /locus_tag="ACHKTW_005390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365813.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PrgH/EprH family type III secretion apparatus
                     protein"
BASE COUNT          220 a          116 c           90 g          207 t
ORIGIN      
        1 ataaaacttc ataattcctg tttttgttta caagaatttt atcgatatgt ctcagctgcg
       61 gatcattact agcattaaca atataaaaag caataaaaat gatagcaaaa agaaaaaaaa
      121 aagcattaca tacttaaaca gcttactgct aaagaaatgt tgttttgtag aaataatgtt
      181 attttcaata atgcctggca atgaatactg ccaaacttca tttttttctt ttacagcaaa
      241 tttgagacct aaaacttcta tttgctgatt caatgttaac gttctatctt caggttcttg
      301 tgctgttaat tctctgaatg ttactaccag atcagggtcg gtagtaatta ttatctcaaa
      361 actacctgta agctcgccat aaggaacgac tattgtgttt tctggtaact cagaaaaagc
      421 atcacttctt cctaacgaaa catcattacc aatgataaca agtagacgcc cattaagtat
      481 ttcatactcg catccattca atggtccgct caacaaacga atggcataag attccaataa
      541 gtcttgtgat aagaatttat cattattttc catgaatacc acccgctaat tccataagct
      601 ctagtttaga gtagtgtaac aagacggaaa ttt
//
LOCUS       DAZFQD010000384          632 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.391, whole genome shotgun sequence.
ACCESSION   DAZFQD010000384 DAZFQD010000000
VERSION     DAZFQD010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..632
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.391"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..368
                     /locus_tag="ACHKTW_005391"
     CDS             <1..368
                     /locus_tag="ACHKTW_005391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000267285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO6466216.1"
                     /translation="GTYATHLGGLWRAYEKTHGMRGWECLVDGVADIDVSMTGERLFS
                     VVVRQSSGQRTEKTFSLPVMLYRGVFRAGETYHPGDTVTWGGSLWHSNSMTGDKPGEA
                     HSSGWTLAAKRGRDAGGGK"
     gene            365..>632
                     /locus_tag="ACHKTW_005392"
     CDS             365..>632
                     /locus_tag="ACHKTW_005392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFO6466217.1"
                     /translation="MTALLTLEEIKAHLRVDHDADDDMLMEKVRQATAVLLAYIQGSR
                     DKVIREDGELIPGEALTRMKGAAMRLTGMLYRNPDLAEREELLQG"
BASE COUNT          139 a          149 c          227 g          117 t
ORIGIN      
        1 ggggcacgta tgccacacac ctgggcggac tctggcgggc gtatgaaaaa acgcacggga
       61 tgcggggatg ggaatgtctg gttgacgggg tggcggatat tgacgtcagc atgacgggtg
      121 aacggttgtt ctctgtggtg gtccggcaga gcagtggcca gcgtacggaa aaaacatttt
      181 ccctgccggt gatgctctac cgcggtgtgt tcagagccgg tgaaacctac caccccggcg
      241 atacggtgac gtggggcggc tcgctgtggc acagcaacag tatgaccggt gataaacccg
      301 gagaagctca ttcatcaggc tggaccctgg ctgcaaaacg tgggcgggat gctggaggcg
      361 gaaaatgacg gcattactga cactggaaga gatcaaggca catctgcgtg tcgaccatga
      421 cgcggatgat gacatgctga tggagaaggt tcgtcaggct accgccgtgc tgctggccta
      481 cattcagggc agccgggata aggtgattcg tgaggacggt gaactgatcc cgggcgaggc
      541 attaacccgg atgaaggggg ctgccatgcg actgaccggg atgctgtacc ggaatccgga
      601 tcttgcggag cgggaagaac tgcttcaggg gg
//
LOCUS       DAZFQD010000385          628 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.392, whole genome shotgun sequence.
ACCESSION   DAZFQD010000385 DAZFQD010000000
VERSION     DAZFQD010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..628
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.392"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(1..628)
                     /locus_tag="ACHKTW_005393"
                     /pseudo
     CDS             complement(1..628)
                     /locus_tag="ACHKTW_005393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000097604.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          209 a          137 c           81 g          201 t
ORIGIN      
        1 gcgtaccttg ttatcattaa atataaattc gggtaaatgc agtatgcatt ttttgtcatt
       61 caagcaacta tcaataaatt cttttttaaa agacaaaaaa ctccatgata aaaaaccacc
      121 ctgccctgaa ttttgttgtc agattgtaga ttaacgccaa ataacgtctt tacatcttcc
      181 ctgagattat agaatgccgt atcaacaaga gcctcgatcg tttgtttatc aacaatttta
      241 ataaactctt cgtatacttg cttttgcgtc ttatggccgc ccagcgccca tgaatactca
      301 tgttctttta aaaccagtat ggctcttaac agacaatatc catcgccatc tactggagcg
      361 tattttaatc catatgtttt attaatttca ctctgaataa aagatatgcc agacaaatca
      421 ataccagtat ctaaatcaga tattcccggc gtatttatct gttctggctg gatactcata
      481 ttttcatcat caaaacaata ctcagaaaaa acatcatcac acgcagtaat gttttcaata
      541 tattccataa agccatacca ataataacct ttaaaatctg tgatagactc tgaacctctg
      601 cccggcaagg acactctaac aatatcaa
//
LOCUS       DAZFQD010000386          623 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.393, whole genome shotgun sequence.
ACCESSION   DAZFQD010000386 DAZFQD010000000
VERSION     DAZFQD010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..623
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.393"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..253
                     /locus_tag="ACHKTW_005394"
     CDS             <1..253
                     /locus_tag="ACHKTW_005394"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6466218.1"
                     /translation="HDQHHHIQFRHTGNQTHLSGLPDERHPGTTKRWRKNPGISTGGY
                     RHPEMHHIRLINGSHFCATNMEEVRVLTATRNTGGKAWQ"
     gene            244..594
                     /locus_tag="ACHKTW_005395"
     CDS             244..594
                     /locus_tag="ACHKTW_005395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4406 domain-containing protein"
                     /protein_id="HFO6466219.1"
                     /translation="MAVIYIAGPMTGCKDHNRTAFCVEDLKLRCAGNIVLNPAVLPDG
                     LSQQQYMSICIPMPMCADAIYLLDGWEESAGARAEYAMALKLNMPVSFPENRAAGETV
                     AQLLHKSKAAQATE"
BASE COUNT          168 a          176 c          165 g          114 t
ORIGIN      
        1 gcatgaccag catcaccata tccaattccg acacaccgga aaccagacgc atttatcagg
       61 gttaccggat gaacgccatc caggcaccac gaagcgttgg cgcaagaacc cgggaatcag
      121 caccggaggt tatcgccacc ctgagatgca tcacatccgc cttatcaacg gttcgcactt
      181 ctgcgccacg aacatggaag aagtacgcgt gctgacagca acacgcaata cgggaggtaa
      241 agcatggcag tgatttatat cgccggtccc atgaccggat gcaaagacca caacaggacg
      301 gcattttgcg tggaggacct gaaacttcgg tgcgccggaa acatcgttct taatcctgcc
      361 gtactgcctg acggattaag ccagcagcag tacatgagta tttgtattcc gatgcccatg
      421 tgtgccgatg ccatttactt actggatgga tgggaagagt ccgctggcgc acgggctgag
      481 tacgcaatgg cgctcaaact gaacatgcca gtgtcattcc cggaaaaccg ggcagccggc
      541 gaaaccgtcg cgcagctgct tcacaaatca aaagccgcac aggcgactga gtaaccgacc
      601 ggagatcgaa acatgattgc gcg
//
LOCUS       DAZFQD010000387          598 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.394, whole genome shotgun sequence.
ACCESSION   DAZFQD010000387 DAZFQD010000000
VERSION     DAZFQD010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..598
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.394"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>598)
                     /locus_tag="ACHKTW_005396"
     CDS             complement(<1..>598)
                     /locus_tag="ACHKTW_005396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFO6466220.1"
                     /translation="YLDADIACKGSIKELLDYQFSTNEIAAVVAERDVEWWQNRASVL
                     TTPQLASGYFNAGFLLINIDEWNLNNISSKAIEMLRDPDWVSKITHLDQDVLNVLLNG
                     KVKFISEKYNTRYSINYELKDKVDNPVNDDTVFIHYVGPTKPWHEWADYPVSRSFLIA
                     KAASPWSKEDLLKPVNSNQYRYCAKHKFKQKHYMAGIFN"
BASE COUNT          187 a          119 c           92 g          200 t
ORIGIN      
        1 aattaaaaat gcctgccata taatgctttt gtttaaattt atgttttgca caataccgat
       61 actgattgct atttacaggt ttaagtaaat cttctttact ccacggagaa gctgctttgg
      121 cgatcaaaaa actacgtgac accggatagt cagcccactc atgccagggt tttgtaggtc
      181 ccacatagtg tataaacacg gtgtcatcat tgactggatt atcaactttg tcttttaatt
      241 catagttaat actatatcgg gtattatatt tctccgaaat aaacttcact ttaccattca
      301 ataatacatt cagtacatct tgatcaaggt gggtgatttt acttacccaa tctgggtcac
      361 gcaacatttc aatagctttt gacgaaatgt tatttagatt ccactcatca atatttatca
      421 gtaaaaaacc agcattgaaa tatccagaag ctaactgtgg tgtagttaat accgaggctc
      481 gattttgcca ccattcaaca tctctttcag ctacaactgc ggcaatttca ttagtagaaa
      541 attgataatc taagagttct ttaatactac ccttgcaagc aatatctgca tcaagata
//
LOCUS       DAZFQD010000388          596 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.395, whole genome shotgun sequence.
ACCESSION   DAZFQD010000388 DAZFQD010000000
VERSION     DAZFQD010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..596
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.395"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..308
                     /gene="elaD"
                     /locus_tag="ACHKTW_005397"
     CDS             <1..308
                     /gene="elaD"
                     /locus_tag="ACHKTW_005397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="deubiquitinase ElaD"
                     /protein_id="HFO6466221.1"
                     /translation="ADLQQYLSQSCGAFVCMAAQEVIEQRESNSDSAPYTLLKNYADR
                     FKKYSAEEQYEIDFQHRLINRNCYLDKYGDANINHYYKNLEIKHTQPKNRASGKRVS"
     gene            complement(396..>596)
                     /locus_tag="ACHKTW_005398"
     CDS             complement(396..>596)
                     /locus_tag="ACHKTW_005398"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6466222.1"
                     /translation="YSGFAILKRNSYKEKYNYVIYWQIELTGGRNLMVRKVSNNNPLH
                     QQTSNRVPRYAGNTRRLNWRMV"
BASE COUNT          200 a          116 c          107 g          173 t
ORIGIN      
        1 atgccgatct tcagcaatac ttaagccaaa gttgcggtgc gtttgtgtgc atggcagccc
       61 aggaagtgat tgaacaaagg gaaagcaatt ctgacagcgc cccctatacg ttattaaaaa
      121 actatgctga cagatttaaa aaatattcag cagaagagca gtacgaaatt gattttcaac
      181 atcgactgat aaacagaaat tgttatttag ataaatatgg cgatgctaat atcaatcatt
      241 attataaaaa cttagaaata aaacacacac aacccaaaaa tagagcgtca ggaaaaagag
      301 taagttaaca tcattgttaa ttaattcgac cagtcagtaa atatgctgac tggtaggaca
      361 tagtttgcta aaaagtcatc actgactgat gccagtcaca ccatccgcca gttcaatctt
      421 cgggtatttc cagcgtatct cggcacgcga ttgctggtct gttgatgtag tggattgttg
      481 ttgctgactt ttcttaccat cagatttctg ccccctgtca actcaatctg ccaataaata
      541 acataattat atttttcctt atatgaattc ctttttaaaa tagcgaagcc actgta
//
LOCUS       DAZFQD010000389          592 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.396, whole genome shotgun sequence.
ACCESSION   DAZFQD010000389 DAZFQD010000000
VERSION     DAZFQD010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..592
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.396"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            18..509
                     /gene="ygeG"
                     /locus_tag="ACHKTW_005399"
     CDS             18..509
                     /gene="ygeG"
                     /locus_tag="ACHKTW_005399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFO6466223.1"
                     /translation="MSTETIEIFNNSDEWANQLKHLLSKGENLALLHGLTPDILDRIY
                     AYAFDYHEKGNVTDAEIYYKFLCIYAFENHEYLEGFASVCQSKKKYQQAYDLYKLSYN
                     YFPYDDYSVIYRMGQCQIGAKNIDNAMQCFYHIINNCEDDSVKSKAQAYIELLNDNSE
                     DNG"
BASE COUNT          216 a           91 c          103 g          182 t
ORIGIN      
        1 catcacagag gaatattatg agcacagaaa caattgaaat attcaataat agtgatgaat
       61 gggcaaatca actaaaacac ctattatcga aaggagaaaa tctggcatta ctacatggtt
      121 taactcctga tatccttgat agaatatatg catatgcatt cgactaccat gaaaagggta
      181 atgtaacgga cgcagaaatt tattataaat ttttgtgcat ttatgcgttc gaaaatcatg
      241 agtatctaga aggttttgca tcagtatgtc agtcaaaaaa gaaatatcaa caagcatatg
      301 acctttacaa actaagttac aattacttcc cgtatgatga ctattcagtt atttatcgta
      361 tgggtcaatg tcagattggg gctaaaaata tcgataacgc aatgcaatgt ttctatcaca
      421 ttattaacaa ttgtgaggat gatagtgtta agagtaaagc gcaggcatat attgaactct
      481 taaacgataa ttcagaagat aatggctaag cattacaatc atttggttaa atttgctggt
      541 tatagtgcag ttaaccacta taaccagcac ctcttatgtt ttcttaaatc ag
//
LOCUS       DAZFQD010000390          590 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.397, whole genome shotgun sequence.
ACCESSION   DAZFQD010000390 DAZFQD010000000
VERSION     DAZFQD010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..590
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.397"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..530)
                     /locus_tag="ACHKTW_005400"
     CDS             complement(<1..530)
                     /locus_tag="ACHKTW_005400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6466224.1"
                     /translation="MAYFYFKLDRVQTNKYFTKYQQTVLPLRNSILRALLKNTGAAGL
                     RLKPFAMDVISEFYFSGTLPAGWRKRDDVAFIGDGPCFIARPDESCPEGPAIAAMIEA
                     AERELRKRPDFLVWLCEKLGVMRIPSMFNMDSWWTPSLSRDALCVVFKVGAYGREIKG
                     GIPEECQEIKHSEYVAL"
BASE COUNT          159 a          159 c          127 g          145 t
ORIGIN      
        1 agcgccacat attcagaatg tttaatctcc tggcattctt caggaatacc cccttttatt
       61 tccctgccat aagcgcctac tttaaacacc acgcacaggg catcacggga gagcgacggg
      121 gtccaccagg agtccatgtt aaacatggag gggattctca ttacccccag cttttcacag
      181 agccagacaa ggaaatcagg acgctttctt aactcgcgtt cagcggcttc aatcattgcg
      241 gcaatcgccg gtccttcagg gcatgactca tcaggtctgg caataaagca cggtccgtcc
      301 ccgataaaag ccacatcatc gcgcttacgc cagcccgcag gcagagtacc agaaaaataa
      361 aactcactga ttacgtccat ggcaaacggc tttaagcgca atccggcagc gcctgtattt
      421 ttcagtaacg cccgaagaat actgttgcgt agcggtaaaa cagtctgttg atatttggtg
      481 aaatatttat ttgtctgcac acggtctaat ttgaaataaa aatatgccat atatcctgtt
      541 ccttattttc agtttacagg cgcaggcagt cccctgacgc gagcgccata
//
LOCUS       DAZFQD010000391          585 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.398, whole genome shotgun sequence.
ACCESSION   DAZFQD010000391 DAZFQD010000000
VERSION     DAZFQD010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..585
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.398"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            296..490
                     /locus_tag="ACHKTW_005401"
     CDS             296..490
                     /locus_tag="ACHKTW_005401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000142785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HFO6466225.1"
                     /translation="MTFIHQVMLYFCTAVCVLYLLSGGYRAVRDSWRRQIDKRAAEKI
                     SASQSAGAKTEAPLIPEQPS"
     gene            516..>585
                     /locus_tag="ACHKTW_005402"
     CDS             516..>585
                     /locus_tag="ACHKTW_005402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HFO6466226.1"
                     /translation="MSEIKSLVTAEAVKEVLRSEEVR"
BASE COUNT          179 a          129 c          150 g          127 t
ORIGIN      
        1 cagagcagac ggcatttgac cttgtgatgt ggacaaatga tggctttaca gcagatacgc
       61 tgacgctgac agatatcacg gggacaaaag cgacgtatcc ggtactgctt gatacggtca
      121 ctgtcaacgt taacgaaagc cgggcatcag cataaccggc aaaaaaaaac cgccagcggc
      181 aggaacggaa gctggcggag gtaatcccaa tggagaatgt aaagaaaaga tgctttcgta
      241 cattggtttt ttaaatgaaa acagttctca ttgtcaacca taacggtaag aaactatgac
      301 atttattcat caggtgatgc tgtacttctg tacggcggtc tgtgtgctgt atcttctttc
      361 gggtgggtac agggcagtgc gcgattcctg gcgcaggcag attgataaaa gggccgcaga
      421 gaaaatcagc gccagtcagt cagccggagc aaaaacagaa gccccactca ttccggaaca
      481 accttcttaa taaccccttt caacgagaaa atcctatgtc agaaataaaa tcgctggtca
      541 ctgctgaggc agtgaaggaa gtcctgcgct ctgaagaagt cagaa
//
LOCUS       DAZFQD010000392          585 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.399, whole genome shotgun sequence.
ACCESSION   DAZFQD010000392 DAZFQD010000000
VERSION     DAZFQD010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..585
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.399"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>585
                     /locus_tag="ACHKTW_005403"
     rRNA            <1..>585
                     /locus_tag="ACHKTW_005403"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          134 a          131 c          194 g          126 t
ORIGIN      
        1 tggtgggtag tttgactggg gcggtctcct cctaaagagt aacggaggag cacgaaggtt
       61 ggctaatcct ggtcggacat caggaggtta gtgcaatggc ataagccagc ttgactgcga
      121 gcgtgacggc gcgagcaggt gcgaaagcag gtcatagtga tccggtggtt ctgaatggaa
      181 gggccatcgc tcaacggata aaaggtactc cggggataac aggctgatac cgcccaagag
      241 ttcatatcga cggcggtgtt tggcacctcg atgtcggctc atcacatcct ggggctgaag
      301 taggtcccaa gggtatggct gttcgccatt taaagtggta cgcgagctgg gtttagaacg
      361 tcgtgagaca gttcggtccc tatctgccgt gggcgctgga gaactgaggg gggctgctcc
      421 tagtacgaga ggaccggagt ggacgcatca ctggtgttcg ggttgtcatg ccaatggcac
      481 tgcccggtag ctaaatgcgg aagagataag tgctgaaagc atctaagcac gaaacttgcc
      541 ccgagatgag ttctccctga ccctttaagg gtcctgaagg aacgt
//
LOCUS       DAZFQD010000393          575 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.400, whole genome shotgun sequence.
ACCESSION   DAZFQD010000393 DAZFQD010000000
VERSION     DAZFQD010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..575
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.400"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(351..437)
                     /locus_tag="ACHKTW_005404"
     tRNA            complement(351..437)
                     /locus_tag="ACHKTW_005404"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(401..403),aa:Leu,seq:taa)
     gene            complement(450..523)
                     /locus_tag="ACHKTW_005405"
     tRNA            complement(450..523)
                     /locus_tag="ACHKTW_005405"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(489..491),aa:Cys,seq:gca)
BASE COUNT          165 a          161 c          124 g          125 t
ORIGIN      
        1 acgggggagc aatcccccgt caatcggttg ccaaggtaag gccgaaagta gcaccctcac
       61 acgcaagtca caaagaaaaa caaagcccgt agcgtaaaaa ctgcgggctt ttcttttcag
      121 catcattgaa agcaatccac accaacaaac tgtggataac accgagagta gtaaaagcgc
      181 agttatgaca gcaaaggcaa ttgctactga gcctgcttta ctcttgctcc tgcatatcgc
      241 cataccatcg ccacgttacc gccacataca aaaacacaag aacaaaaaaa ccacccgaag
      301 gtggtttcac gacactgctt attgctttga ttttattctt atctttccca tggtacccgg
      361 agcgggactt gaacccgcac agcgcgaacg ccgagggatt ttaaatccct tgtgtctacc
      421 gattccacca tccgggctcg ggaagaaagt ggaggcgcgt tccggagtcg aaccggacta
      481 gacggatttg caatccgcta cataaccgct ttgttaacgc gccaaattct tcaggccttt
      541 cagccagaca tccgctgacg ccgatgtctt ttaaa
//
LOCUS       DAZFQD010000394          573 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.401, whole genome shotgun sequence.
ACCESSION   DAZFQD010000394 DAZFQD010000000
VERSION     DAZFQD010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..573
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.401"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            34..>573
                     /locus_tag="ACHKTW_005406"
     CDS             34..>573
                     /locus_tag="ACHKTW_005406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000555106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFO6466227.1"
                     /translation="MHRVCLEANRYLDKYDAMAKEVNKLLGRIAPPAPVFNGEYGDAY
                     QGAREDLSIWKRRALEADEHVRRLEQINDHMVKEAQGESRMGEPVIREPAPVVPEEAT
                     PENVEMLSGYVSTYKLTDSERDIAAEIWNACRAAMLQSGNFRENKNSSTNNFREIAET
                     STNYPAIPSEVLSAILKVAR"
BASE COUNT          166 a          137 c          154 g          116 t
ORIGIN      
        1 gcagatccag ttaaacgagt taactcagat cagatgcacc gagtctgctt agaagctaat
       61 cgctatttag ataaatatga cgcgatggcg aaagaggtaa ataagttgct tggacgcatc
      121 gccccgccag cgccagtatt taacggcgaa tacggtgacg catatcaggg cgctcgtgaa
      181 gacctgtcca tctggaaacg gcgagcgctt gaagctgatg agcacgttcg gcgactggag
      241 caaatcaatg accacatggt gaaagaggcg cagggagaat cacgcatggg cgagcctgta
      301 atacgtgagc cagcaccggt agtgcctgaa gaagcaactc cggaaaacgt agaaatgctc
      361 tctggctatg tttcaacgta caaattaacc gatagcgagc gcgatattgc tgccgaaata
      421 tggaacgcct gccgcgccgc catgcttcag tccggaaact ttcgggaaaa caagaattcg
      481 tcaaccaata attttcggga aatcgcggaa acgtcaacca actatccggc aattcctagt
      541 gaggtgttgt ccgcaatcct gaaggttgcc agg
//
LOCUS       DAZFQD010000395          573 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.402, whole genome shotgun sequence.
ACCESSION   DAZFQD010000395 DAZFQD010000000
VERSION     DAZFQD010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..573
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.402"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
BASE COUNT          172 a          108 c           87 g          206 t
ORIGIN      
        1 tccttctaat acctattatt acatataaag aaacatttgt caataccttt tcataaaaaa
       61 tctgatcttt ttcatgcact tacatttttt cttagcaatt cgtgctctcc tatgtagtgc
      121 tattcttcat tattgtctaa aacgctctaa aacgcgttct aacgaggttt tgataaaagc
      181 aatacattcc cttatctaat ggctgtttag ctcgccagag gcttatttaa ggggcttatt
      241 ttgtatttgt taggtatatg aaaaggtgat ggtagatata agatagagag tcactttata
      301 aaaacgctct gtaaagctct gtagtgagct tattatatgt aggcaatgcg tttccattcc
      361 ctaccctgct taatgctgtt agagaagcat ttagacccct tatttttaat taattaaagt
      421 atcctctata cactcctgtt atgaaacacc tctgaatcct tcataaaaca cattatgacg
      481 caaagaacac cttttctaag gtgattccct tcctgaatgc tgtttcttgc gtcagagagc
      541 tacagagagc ttataaagct attattaata ata
//
LOCUS       DAZFQD010000396          568 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.403, whole genome shotgun sequence.
ACCESSION   DAZFQD010000396 DAZFQD010000000
VERSION     DAZFQD010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..568
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.403"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>568
                     /locus_tag="ACHKTW_005407"
     CDS             <1..>568
                     /locus_tag="ACHKTW_005407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HFO6466228.1"
                     /translation="KDHITITDSLNRREVLHTQGEAGLKRVVKKEHADGSVTQSQFDA
                     VGRLKAQTDAAGRTTEYSPDVVTGLITRITTPDGRASAFYYNHHSQLTSATGPDGLEL
                     RREYDESGRLIQETAPDGDITRYRYDNPHSDLPCATEDATGSRKTMTWSRYGQLLSFT
                     DCSGYQTRYDHDRFGQMTAVHREEGLSQY"
BASE COUNT          140 a          168 c          173 g           87 t
ORIGIN      
        1 aaagaccaca tcaccatcac cgacagcctg aaccgccgtg aagtgctgca cacgcagggc
       61 gaagccgggc tgaagcgggt ggtgaaaaag gaacacgcgg acggcagcgt cacgcagagt
      121 cagtttgatg cggtgggcag gcttaaggca cagacggatg ccgcaggcag gacaacagag
      181 tacagcccgg atgtggtgac gggactcatc acgcgcatca ccacgccgga tggcagggca
      241 tcggcgtttt actataacca ccacagccag ttaacgtcag ccaccgggcc tgacgggctg
      301 gaattgcgcc gggaatatga tgaatccggc cgtctgattc aggaaactgc ccctgacggc
      361 gatatcaccc gctaccgtta tgataatcca cacagtgact taccctgcgc aacggaagat
      421 gccaccggca gccggaaaac catgacgtgg agccgttacg gccagttgct gagcttcacc
      481 gactgttccg gctaccagac ccgttatgac catgaccgct tcgggcagat gacggcggtg
      541 caccgcgagg aagggctgag ccagtacc
//
LOCUS       DAZFQD010000397          568 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.404, whole genome shotgun sequence.
ACCESSION   DAZFQD010000397 DAZFQD010000000
VERSION     DAZFQD010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..568
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.404"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>568
                     /locus_tag="ACHKTW_005408"
     CDS             <1..>568
                     /locus_tag="ACHKTW_005408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HFO6466229.1"
                     /translation="KDRITITDSLNRREVLHTQGEAGLKRVVKKEHADGSVTQSQFDA
                     VGRLKAQTDAAGRTTEYSPDVVTGLITRITTPDGRASAFYYNHHNQLTSATGPDGLEL
                     RREYDESGRLIQETAPDGDITRYRYDNPHSDLPCATEDATGSRKTMTWSRYGQLLTFT
                     DCSGYVTRYDHDRFGQVTAVHREEGLSQY"
BASE COUNT          137 a          168 c          171 g           92 t
ORIGIN      
        1 aaagaccgca tcaccatcac cgacagcctg aaccgccgtg aagtgctgca cacgcagggc
       61 gaagccgggc tgaagcgggt ggtgaaaaag gaacacgcgg acggcagcgt cacgcagagt
      121 cagtttgacg cggtgggcag gcttaaggca cagacggatg ccgcaggccg gacaacagag
      181 tacagcccgg atgtggtgac gggcctcatc acgcgcatca ccacgccgga tggcagggca
      241 tcggcgtttt actataacca ccacaaccag ttaacgtcag ccaccgggcc tgacgggctg
      301 gaattgcgcc gggaatatga tgaatccggc cgtctgattc aggaaaccgc ccctgacggc
      361 gatatcaccc gctaccgtta tgataatcca cacagtgact taccctgcgc aacggaagat
      421 gccaccggca gccggaaaac catgacgtgg agccgttacg gtcagttgct gaccttcact
      481 gactgttccg gttatgtaac ccgctatgac catgaccgtt ttggtcaggt gacggcggtg
      541 caccgcgagg aaggcctgag ccagtacc
//
LOCUS       DAZFQD010000398          567 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.405, whole genome shotgun sequence.
ACCESSION   DAZFQD010000398 DAZFQD010000000
VERSION     DAZFQD010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..567
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.405"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(5..>567)
                     /gene="wzx"
                     /locus_tag="ACHKTW_005409"
     CDS             complement(5..>567)
                     /gene="wzx"
                     /locus_tag="ACHKTW_005409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001242528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="O91 family O-antigen flippase"
                     /protein_id="HFO6466230.1"
                     /translation="QIIIRTYIANHFGWVQAGIWDGVWRISSLYLMLVTVPLGVYYLP
                     RLAELHTKSLLLREIKQGYLFLLPIIIISAIIIYYLRYFIIELLFSKSFVSMGDIIGW
                     QLLGDIFKIGSWLLSYIMLSKALTKTYIITEITTALLFLGGVHYLSPLYGLKGVTLAY
                     CITYFCYWIMMIVVIISYFKGNFRNE"
BASE COUNT          226 a          107 c           73 g          161 t
ORIGIN      
        1 aaagctattc atttctgaag ttccccttaa aataagaaat tattacaact atcatcataa
       61 tccagtaaca aaaataggtt atacaatacg ccagagtaac accttttaaa ccatataaag
      121 gcgaaagata gtgtactccg cctaaaaaaa gtaatgcagt tgtaatctct gtaataatat
      181 atgtcttcgt taatgcttta ctcaacataa tgtaagaaag tagccaactt ccaattttaa
      241 atatatcacc aagtaactgc cagccaataa tatcccccat agatacgaat gactttgaaa
      301 aaagcaattc aataataaaa tatctaagat aataaattat aatagctgaa ataattataa
      361 ttggaagaag aaacagatat ccttgtttaa tctctcttaa aagcaacgac ttagtatgta
      421 gttctgcaag ccttgggaga taatataccc ctagaggaac ggtcactaac attaaatata
      481 atgaacttat acgccagaca ccatcccaaa tacctgcttg cacccatcca aaatgattcg
      541 cgatgtatgt tctaataata atctgca
//
LOCUS       DAZFQD010000399          544 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.406, whole genome shotgun sequence.
ACCESSION   DAZFQD010000399 DAZFQD010000000
VERSION     DAZFQD010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..544
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.406"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            30..>544
                     /locus_tag="ACHKTW_005410"
     CDS             30..>544
                     /locus_tag="ACHKTW_005410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO6466231.1"
                     /translation="MKKSVLTAFITVVCATSSVMAADDNAITDGKVTFNGKVIAPACT
                     LVAATKDSVVTLPNVSATKLQTNGAVSGVKTDVPIALEGCDVTVTKNATFTFSGTADG
                     VQPTAFANQATTDAATNVALQMYLPDGSTSVTPGTETSNIQLADSAEQTVTFKVDYIA
                     TGKATSGNVNAV"
BASE COUNT          157 a          110 c          133 g          144 t
ORIGIN      
        1 atatttacga agagatcagg atgatgacga tgaaaaaaag tgtattgacg gcgtttataa
       61 ctgtggtatg tgcaacgtcc agcgttatgg cagctgatga taatgctatc acggatggca
      121 aagtaacatt taatggtaaa gttattgctc cagcttgtac cctggtagct gcgacgaaag
      181 attcagtagt cacattgcca aatgtcagcg ccaccaagtt gcaaactaat ggagcagtct
      241 ctggcgttaa aactgatgta ccaattgcct tagaaggctg tgatgttact gtaactaaaa
      301 atgcgacgtt tacctttagt ggtacggctg acggcgtgca acctacagca tttgccaacc
      361 aggctaccac cgatgcagcg acgaatgttg ctctacaaat gtatctgcca gatggttcta
      421 catcggtaac tcccggtacc gaaacgtcga atattcagtt agcggattct gctgagcaaa
      481 cagtgacctt taaagttgat tatatcgcta cggggaaagc gacttctggt aatgtgaatg
      541 cggt
//
LOCUS       DAZFQD010000400          534 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.407, whole genome shotgun sequence.
ACCESSION   DAZFQD010000400 DAZFQD010000000
VERSION     DAZFQD010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..534
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.407"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..125
                     /locus_tag="ACHKTW_005411"
                     /pseudo
     CDS             1..125
                     /locus_tag="ACHKTW_005411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278583.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          177 a          121 c           88 g          148 t
ORIGIN      
        1 gaccagatat tgcagtcagg tattactaca aaggcgtttt gtggagtgct ggctattttg
       61 ccagtagctg cggaggtgcg ccaatatccg tcatccgcca atacattgaa caacagcaaa
      121 cacctggtca ggtggaaaac cgcgccttat atccctgccc tgaaggacgg ggttttacgg
      181 cgcaccggat aagcctggag attatctaca ccagcatgga catcattgta ctatgccccg
      241 tacatgcccc atcacatcct caacatcaaa gtattagagc cttgcaaacc attatgcgtc
      301 tcagtttgtc tcatctatct tacgcaaaaa aaatagaact aatatcaagc ggcgctatat
      361 tgcgtattaa ataaaaccac ctcaattaca ttaataaatc caaacaaaca tcatgcaaat
      421 aatttccatg gttatttcta ggctatttaa attaaataaa ttatgagtta atcataaata
      481 acaaccttta atataatttg actaccaaat ttatattcgt tcacaaaata gtca
//
LOCUS       DAZFQD010000401          530 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.408, whole genome shotgun sequence.
ACCESSION   DAZFQD010000401 DAZFQD010000000
VERSION     DAZFQD010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..530
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.408"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(29..256)
                     /locus_tag="ACHKTW_005412"
     CDS             complement(29..256)
                     /locus_tag="ACHKTW_005412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000013474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="derepression protein"
                     /protein_id="HFO6466232.1"
                     /translation="MANRKQQRARAERLHIRTEINRRLFRASRVAQIMHINMLHERTH
                     ALSNRYCAAVFSYLADDLAELQDLINQQHHH"
BASE COUNT          113 a          131 c          172 g          114 t
ORIGIN      
        1 cgggtgcggg atggcttcag gatgttgttt agtggtggtg ttgctggttg ataaggtcct
       61 gcagctccgc gagatcatcc gccaggtagc tgaaaacagc ggcacagtaa cggtttgaca
      121 gagcgtgagt gcgctcatgc agcatattga tgtgcatgat ttgcgcgacg cgtgaggcgc
      181 ggaaaagtct gcggttgatt tcagtgcgga tgtgaagacg ctcagcgcgg gcgcgctgtt
      241 gcttgcggtt tgccatggtg tggcctcttt gctagtaagt tctgaaaact caccatccag
      301 agctgcgaaa ctgtgggtgg cgagacgtac gaggttcgca gtaccggcta gcaaagaacc
      361 cggcccgacc gaagtcggcc ccgtacgccc cgccattatt ctgacgcgaa aaagacgtgg
      421 cgatacagta cgcacaaaaa aaccgctggc gcggttgtgc gctttgctag tcggcgggct
      481 gcgaaacccg gcacccgttt tatgagatgc gggaaaactg taacccgacc
//
LOCUS       DAZFQD010000402          524 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.409, whole genome shotgun sequence.
ACCESSION   DAZFQD010000402 DAZFQD010000000
VERSION     DAZFQD010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..524
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.409"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(29..250)
                     /locus_tag="ACHKTW_005413"
     CDS             complement(29..250)
                     /locus_tag="ACHKTW_005413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000013462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="derepression protein"
                     /protein_id="HFO6466233.1"
                     /translation="MANRKQQRARAERLHIRSEINRRLFRATRVAQIMHINMLHERTH
                     ALSNRYCAAVFSYLAEDLAELQSLINQHR"
BASE COUNT          114 a          125 c          167 g          118 t
ORIGIN      
        1 caggtgtggg atagcttccg gatgtggttt atcggtgctg gttgatgagg ctttgcagtt
       61 ctgccaggtc ttcggccaga tagctgaaga cggcagcgca gtaacggttt gatagtgcgt
      121 gcgtgcgctc atgcagcatg ttgatatgca tgatttgcgc gacgcgtgtt gcgcggaaaa
      181 gtctgcggtt gatttcgctg cggatgtgaa gacgctcagc gcgggcgcgc tgttgcttgc
      241 ggtttgccat ggtgtggcct ctttgctagt aagttttgaa aactcaccaa ccggaggtgg
      301 aaaactcggg gtggcgagac gtacagggtt tccacaaccg gctagcaaag aacccggccc
      361 gaccgaagtc ggccccgtac gccccgccat aattctgatg cgaaaaaaac gtggcaatac
      421 agtacgcaca aaaaaaccgc tggcgcggta gtgcgctttg ctagtcagca gggtggaaat
      481 cccggcaccc gttttatgag gtgcagcgga aatgtaaccc gaca
//
LOCUS       DAZFQD010000403          523 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.410, whole genome shotgun sequence.
ACCESSION   DAZFQD010000403 DAZFQD010000000
VERSION     DAZFQD010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..523
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.410"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..228
                     /locus_tag="ACHKTW_005414"
     CDS             <1..228
                     /locus_tag="ACHKTW_005414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6466234.1"
                     /translation="VKGDGDGFGLFHINGTDSTAISWSSSEMALVLEMTTHSVAVKGI
                     YTAEDFIGEYQRLLVLYRAAFQEVTGGEYDA"
     gene            225..>523
                     /locus_tag="ACHKTW_005415"
     CDS             225..>523
                     /locus_tag="ACHKTW_005415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001279669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HFO6466235.1"
                     /translation="MSDRNAQLAYPWNAPLPVIDPETFGRAQPTALRQAIQSYINEDI
                     RIDARLDEETVSFLTNTDEGKRINSHLHHDEERRVRLEKLAKHKRENPPTVVSEA"
BASE COUNT          142 a          143 c          142 g           96 t
ORIGIN      
        1 gtgaaaggtg acggtgatgg ctttggttta tttcacatca acggcaccga cagtaccgcc
       61 atcagctggt catcgtcaga gatggcactg gttctggaaa tgacaaccca cagcgtggcc
      121 gtgaaaggca tatacaccgc agaagacttt atcggtgaat accagcgcct tctcgtgctg
      181 taccgcgcgg cgtttcagga ggtcactggc ggggagtacg acgcatgagt gaccgcaatg
      241 cacagctggc gtacccgtgg aacgcaccgc taccggttat cgatcctgaa acgttcggca
      301 gagcacaacc aaccgcatta cgccaggcca ttcagagcta catcaacgag gacattcgca
      361 ttgatgccag actggatgaa gaaaccgtct ccttcctgac aaacacagac gaaggcaagc
      421 gaattaattc gcatcttcac cacgacgaag agcgtcgggt cagactggaa aaactggcga
      481 agcacaaacg cgagaatccg cccacggtgg tatcggaagc cat
//
LOCUS       DAZFQD010000404          517 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.411, whole genome shotgun sequence.
ACCESSION   DAZFQD010000404 DAZFQD010000000
VERSION     DAZFQD010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..517
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.411"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(80..>517)
                     /locus_tag="ACHKTW_005416"
     CDS             complement(80..>517)
                     /locus_tag="ACHKTW_005416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000686886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFO6466236.1"
                     /translation="DDSKPLKNYASRDYHHNKRTILRVGTVEIICKRESILKTDLFPE
                     DMGAGALFPLCDEPVFLNNCLKQSLKVQFLSIPLCYHPNVSSGSNLNRCYTIARGVCF
                     RRIYGMPALLLILIFSLKMKLKYRKLDLLTYTKSLYRGYNEGK"
BASE COUNT          189 a          100 c           75 g          153 t
ORIGIN      
        1 ccacttccat gaaaagaaag gccaaagtga caaaaagcac agaaatccat agaaccaatt
       61 aagatatggt gtcaaaacgc tacttgcctt cattataccc ccgataaaga gatttggtat
      121 aagttagcaa gtctaatttc ctgtacttca gtttcatttt caaagaaaat attaagatca
      181 atagcaatgc aggcatgcca tatatacgtc tgaaacaaac acctcttgca atggtatagc
      241 atctatttaa attactacct gaagaaacat taggatgata acaaagaggt atacttaaaa
      301 actgaacctt gagtgattgt tttaaacaat tatttaaaaa aacaggctca tcacacagag
      361 gaaataaagc accagccccc atgtcctctg gaaataaatc agtcttcaaa atactttcac
      421 gtttacagat tatctctact gtaccaactc ttaatattgt tcttttatta tggtgatagt
      481 cccttgatgc ataatttttc aaaggtttgc tatcatc
//
LOCUS       DAZFQD010000405          511 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.412, whole genome shotgun sequence.
ACCESSION   DAZFQD010000405 DAZFQD010000000
VERSION     DAZFQD010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..511
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.412"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..185
                     /locus_tag="ACHKTW_005417"
     CDS             <1..185
                     /locus_tag="ACHKTW_005417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000752594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFO6466237.1"
                     /translation="DKQYVWCWGKSYGWTECDTFEGYYDCSRNKWWAVTDNGEEPASK
                     VTHWMPLPEPPQEVNQ"
     gene            270..>511
                     /locus_tag="ACHKTW_005418"
     CDS             270..>511
                     /locus_tag="ACHKTW_005418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFO6466238.1"
                     /translation="MFALIQRGQIYTDRAGYPVVITRITEHSVFFRRMDGRSGRVRIT
                     EFNSLFEHIDHQEYRKILADTEQEKHLKKLRAIKRK"
BASE COUNT          140 a          112 c          146 g          113 t
ORIGIN      
        1 acgataaaca gtatgtttgg tgttggggta agtcttacgg ctggactgag tgcgatacct
       61 tcgaagggta ttacgattgt tcgagaaaca aatggtgggc agttactgac aatggggaag
      121 aaccggcatc gaaagtaacc cactggatgc cgctaccgga gccgccgcag gaggtgaatc
      181 aatgagctgg cctgatgcaa tcgtaactct ggggatggta ttcgcagcag cgtttgttgt
      241 gtactcgatt tgtcgatggg gataaccaca tgttcgcttt gattcaacgc gggcagatat
      301 acacggacag agccggatac cccgtggtga ttactcgcat cactgagcac tcagtgttct
      361 ttcgacggat ggacggacga tccgggcggg tacgcatcac tgagttcaac agcctgttcg
      421 aacatattga ccaccaggag taccgcaaaa ttctcgcgga cactgagcag gaaaagcacc
      481 tgaaaaaatt acgagccata aaaaggaagt a
//
LOCUS       DAZFQD010000406          508 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.413, whole genome shotgun sequence.
ACCESSION   DAZFQD010000406 DAZFQD010000000
VERSION     DAZFQD010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..508
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.413"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..508
                     /locus_tag="ACHKTW_005419"
                     /pseudo
     CDS             1..508
                     /locus_tag="ACHKTW_005419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416772.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ulp1 family isopeptidase"
BASE COUNT          182 a          110 c           77 g          139 t
ORIGIN      
        1 tgtttaaaat tatataattt ggcacatacc gcaaccaact ccccccttaa accaactggt
       61 ccggataatt cagatgttct cctgttcaaa aagctattct ccccttcgaa attaatgaca
      121 attatcggtg atgaaattcc tcttatatct gaaaaacagt cgctttcaaa ggtgctttta
      181 aatgatgaga ataatgaact gagtgatggt acaaacttct gggataaaaa tcgtcaatta
      241 accacagatg aaatagcttg ctatcttcag aagatcgccg ctaatgcaaa aaatactcaa
      301 gtcaattatc ctactggtct ctacgtcccc tactccacca gaactcacct ggaagacgct
      361 ctcaatgaaa atattaagag cgatccatca tggccgaatg aagtccagtt attccccata
      421 aatactggcg gacactggat attagtttcg ctacagaaaa tagtaaataa aaaaataata
      481 aactacaaat aaaatgcgtc atattcaa
//
LOCUS       DAZFQD010000407          494 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.414, whole genome shotgun sequence.
ACCESSION   DAZFQD010000407 DAZFQD010000000
VERSION     DAZFQD010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..494
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.414"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..286
                     /locus_tag="ACHKTW_005420"
     CDS             <1..286
                     /locus_tag="ACHKTW_005420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000049300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6466239.1"
                     /translation="VRVIFEFDHVMHEVKPAGNDSEEITEGVTATVKIERDTENRPAG
                     PCDVYAQILKYHSPTIIQFLTDELQGSMQAMGVSSSVERRSVQNGPDTLQ"
     gene            298..>494
                     /locus_tag="ACHKTW_005421"
     CDS             298..>494
                     /locus_tag="ACHKTW_005421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0042262 - DNA protection [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HFO6466240.1"
                     /translation="MAKRVTKLKAAAEAAPQTREEVSRDIRTLGDIQREALRLETAMN
                     DEVAEITARYTPQIENLKKEI"
BASE COUNT          162 a          112 c          126 g           94 t
ORIGIN      
        1 agtacgcgtt atttttgaat ttgaccacgt aatgcatgaa gtaaagccag ccggtaatga
       61 tagtgaggaa atcacggagg gggttactgc cacggtaaaa atcgaacgtg atacagagaa
      121 cagaccggca ggaccctgtg atgtttatgc acaaattctt aaatatcaca gccccacaat
      181 cattcagttt ctgacagatg aactacaggg ttccatgcag gccatggggg taagcagctc
      241 cgttgaacgc cgttctgttc agaacggacc agatacattg caataaggaa aaacaaaatg
      301 gcaaaacgcg ttacaaaatt aaaggccgca gcagaggcgg caccgcagac ccgtgaagag
      361 gtcagccgcg atatccgcac cctgggcgat attcagcgag aggcgctgcg cctggaaaca
      421 gcgatgaatg atgaagtggc agaaatcacc gcccgttata cgccgcagat tgaaaacctt
      481 aaaaaagaaa tcaa
//
LOCUS       DAZFQD010000408          481 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.415, whole genome shotgun sequence.
ACCESSION   DAZFQD010000408 DAZFQD010000000
VERSION     DAZFQD010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..481
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.415"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            17..481
                     /locus_tag="ACHKTW_005422"
                     /pseudo
     CDS             17..481
                     /locus_tag="ACHKTW_005422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612262.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          140 a          130 c          119 g           92 t
ORIGIN      
        1 ggccacctga tgaaaggtga tattctcacc acaacacaaa acaggtgact taatgaacaa
       61 gaaaaccaaa cgaaccttca cccctgagtt caggctggaa tgtgcacagc tgattgttga
      121 taagggctac tcatatcgac aggccagtga agcgatgaat gtcggttcaa ccacgcttga
      181 gagctgggta cgccagctca ggcgagagcg ccagggtatt acgccctctg ccacacccat
      241 tactccagac cagcaacgta tccgcgagct ggaaaagcaa gttcgccgtc tggaggaaca
      301 aaatacgata ttaaaaaagg ctaccgcgct cttaatgtcc gactcgctga acggttcacg
      361 atagccgcca gactaagtga cagccacacg gttgtcagcc tgtgttctgc tctggaaata
      421 caccgcagca gttaccggta ctggcgaaaa cgacgcgata cggttaatcc ggcacgagtc
      481 a
//
LOCUS       DAZFQD010000409          473 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.416, whole genome shotgun sequence.
ACCESSION   DAZFQD010000409 DAZFQD010000000
VERSION     DAZFQD010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..473
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.416"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>473
                     /gene="yfdF"
                     /locus_tag="ACHKTW_005423"
     CDS             <1..>473
                     /gene="yfdF"
                     /locus_tag="ACHKTW_005423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000937827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YfdF"
                     /protein_id="HFO6466241.1"
                     /translation="ATYPESINENNNDEVNGLVQELKNLFNGKEGISTCIKHLLELIK
                     NAIRVNDDPYRFNINNSSVTYIDIGSNDTDHITIGIDNQEPIELPANYKDKELVRTII
                     NDNIVEKTHDINNKEMIFSALKEIYDGDPGFIFDKISHKLRHTVTEFDESGNSEPT"
BASE COUNT          188 a           83 c           78 g          124 t
ORIGIN      
        1 gcaacttacc cagaatccat taatgaaaat aacaatgatg aagttaatgg attagtacaa
       61 gagctcaaaa acctttttaa tggtaaggaa ggaataagca cctgtattaa acatctactt
      121 gagcttataa aaaacgccat acgagtaaac gacgatcctt atagatttaa tattaataat
      181 tcctcagtta cttatattga tattggctcc aatgatacag accatattac tattggtatc
      241 gacaaccaag aaccaataga attacctgcg aactataaag acaaagaact cgtccgtact
      301 atcattaatg acaacatagt tgagaagact catgatatca ataacaagga aatgatcttc
      361 agcgcattaa aagaaatata tgatggagat cctggtttta tcttcgataa gatatcacac
      421 aaactcagac atacggtaac ggaatttgat gagagcggga atagcgaacc aac
//
LOCUS       DAZFQD010000410          472 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.417, whole genome shotgun sequence.
ACCESSION   DAZFQD010000410 DAZFQD010000000
VERSION     DAZFQD010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..472
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.417"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..80)
                     /locus_tag="ACHKTW_005424"
     CDS             complement(<1..80)
                     /locus_tag="ACHKTW_005424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HFO6466242.1"
                     /translation="MSSIDAAVVVDVTRLQRVFARLQFVGG"
     gene            119..206
                     /locus_tag="ACHKTW_005425"
     tRNA            119..206
                     /locus_tag="ACHKTW_005425"
                     /product="tRNA-OTHER"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
     gene            complement(206..>472)
                     /locus_tag="ACHKTW_005426"
     CDS             complement(206..>472)
                     /locus_tag="ACHKTW_005426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HFO6466243.1"
                     /translation="IALTPEDFGRLPAMLAAPDAVLWDNVHQNLLYITETRDGTAKIA
                     VNAPYSVKRQPDKLDVVINAYRVSRNTLIDAVQGGKMEILEGKL"
BASE COUNT          121 a          138 c          107 g          106 t
ORIGIN      
        1 ccccccacaa actgaagccg ggcaaagacc cgctggagac gcgtaacgtc aacaacgaca
       61 gcggcatcaa tggatgacat attgcctccg tgaaactacc tggttaaaat aaaagcaggt
      121 cggtgtactc ttaactggta aagcggcacc tgagggatat cttcacccga aggatgttta
      181 tgtgggttcg acccccacca ccgacttaca atttcccttc gaggatctcc atcttacctc
      241 cctgaacagc atcaatcaga gtattcctgc tcactctgta ggcattaatc accacatcca
      301 gcttatccgg ctgacgtttc acactataag gcgcattgac ggcaatcttt gccgttccgt
      361 cacgggtttc agtgatgtaa agcagattct gatggacatt atcccacagc acggcatcag
      421 gcgcggccag cattgccggt aatcgcccga aatcctccgg cgtcagggcg at
//
LOCUS       DAZFQD010000411          468 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.418, whole genome shotgun sequence.
ACCESSION   DAZFQD010000411 DAZFQD010000000
VERSION     DAZFQD010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..468
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.418"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>468)
                     /gene="wzy"
                     /locus_tag="ACHKTW_005427"
     CDS             complement(<1..>468)
                     /gene="wzy"
                     /locus_tag="ACHKTW_005427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000648148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O91 family O-antigen polymerase"
                     /protein_id="HFO6466244.1"
                     /translation="YYSLFLFLFLLLPRKRIFEISLKFRSIFIFSLLPGLVIYALLII
                     GVKLPYGILDAHNELKDSLGIYYRDYIGTVALSHLVLTVGDSTIIRFSGMLDEPGLLG
                     TISALLLLADKLNFKHKSNYVLLLSGVISISLAFYLLILMGLIFQTRRKIISLA"
BASE COUNT          191 a           81 c           66 g          130 t
ORIGIN      
        1 tgctaaagaa ataattttac gccgtgtttg aaagataagc cccattaaaa ttaataggta
       61 aaaagcaagt gatatactta tcactcctga caataacaat acatagtttg atttgtgttt
      121 aaagtttaat ttatccgcta acagtagaag tgctgaaatt gttcctaaca acccaggttc
      181 atcaagcatt ccagaaaatc gtataatagt tgagtcccct actgttaaaa ctaaatgcga
      241 caaagctaca gttccaatat aatcacgata atagattccc aatgaatcct taagctcatt
      301 gtgagcatca agaataccat atggcagttt aactcctata attaataacg catagataac
      361 caaccccggt aataaagaaa aaataaatat agaacggaac ttcaaagata tctcaaatat
      421 tctttttcta ggtagcaata ggaacagaaa taaaaacaaa gaataata
//
LOCUS       DAZFQD010000412          466 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.419, whole genome shotgun sequence.
ACCESSION   DAZFQD010000412 DAZFQD010000000
VERSION     DAZFQD010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..466
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.419"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            111..353
                     /locus_tag="ACHKTW_005428"
     CDS             111..353
                     /locus_tag="ACHKTW_005428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFO6466245.1"
                     /translation="MFALIQRGQIYTDRAGYPVVITRITEHSVFFRRMDGRSGRVRIG
                     ELNCLFEHIDHQEYRKILADTEQEKHLKKLRAIKRK"
     gene            357..443
                     /locus_tag="ACHKTW_005429"
                     /pseudo
     CDS             357..443
                     /locus_tag="ACHKTW_005429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502644.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          127 a           98 c          130 g          111 t
ORIGIN      
        1 acgacaggca ggaggtgaat caatgagctg gcctgatgca atcgtaactc tgggggtggt
       61 attcgcagca gcgtttgttg tgttctcgat ttgtcgatgg ggataaccac atgttcgctt
      121 tgattcaacg cggtcagata tacacggaca gagctggata ccccgtggtg attactcgca
      181 tcactgagca ctcagtgttc tttcgacgga tggacggacg atccgggcgg gtacgcattg
      241 gtgagttaaa ctgcctgttc gaacatattg accaccagga gtaccgcaaa attctcgcgg
      301 acactgagca ggaaaagcac ctgaaaaaat tacgagccat aaaaaggaag taaagaatga
      361 ataaagcatt tgaacgatgg gtccaccagc gttacggcaa tcgctatgac ctgacgcgag
      421 atgttgacgg cttctactgt cgttaagttg tgaagcgaat gtttga
//
LOCUS       DAZFQD010000413          465 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.420, whole genome shotgun sequence.
ACCESSION   DAZFQD010000413 DAZFQD010000000
VERSION     DAZFQD010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..465
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.420"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..267
                     /locus_tag="ACHKTW_005430"
     CDS             <1..267
                     /locus_tag="ACHKTW_005430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HFO6466246.1"
                     /translation="IALTPEDFGRLPAMLAAPDAVLWDNVHQNLLYITETRDGTAKIA
                     VNAPYGVKRQPDKLDVVINAYRVNKFDIEKAIEGGKLELLEGKL"
     gene            complement(267..347)
                     /locus_tag="ACHKTW_005431"
     tRNA            complement(267..347)
                     /locus_tag="ACHKTW_005431"
                     /product="tRNA-OTHER"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
     gene            386..>465
                     /locus_tag="ACHKTW_005432"
     CDS             386..>465
                     /locus_tag="ACHKTW_005432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HFO6466247.1"
                     /translation="MSSIDAAVVVDVARLQRVFARLQFVGG"
BASE COUNT          113 a          104 c          135 g          113 t
ORIGIN      
        1 attgccctga cgccggagga tttcgggcga ttaccggcaa tgctggccgc ccccgatgcc
       61 gtgctgtggg ataatgtcca ccagaacctg ctttacatca ctgaaacccg tgacggaacg
      121 gcaaagattg ccgtcaatgc gccttatggt gtgaagcgcc agccggataa gctggatgtg
      181 gtaattaatg cttaccgggt aaataaattt gatattgaaa aagcaataga gggcggaaag
      241 ctggaactac tggaaggtaa attgtaagcc ggtgacgggg gtcgaacccg cataaacata
      301 tgacccctga gggtttatgc cgctttacca gttaagagta caccggcctg tatttatttt
      361 aatcagatag ttttaaggag gcaacatgtc atccatcgat gcagccgtgg tggtggacgt
      421 tgcgcgtctc cagcgggtct ttgcccggct tcagtttgtg ggtgg
//
LOCUS       DAZFQD010000414          459 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.421, whole genome shotgun sequence.
ACCESSION   DAZFQD010000414 DAZFQD010000000
VERSION     DAZFQD010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..459
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.421"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            70..411
                     /locus_tag="ACHKTW_005433"
     CDS             70..411
                     /locus_tag="ACHKTW_005433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO6466248.1"
                     /translation="MVILSIDYAKKLRQIRKAEGLTQKEFADITGLSLAAIRNYESGQ
                     KSARAKIVEMVLQVDRFEKYMMWLIKDKTMPAAGQIAPALSLDGSIQSEDDQDSIPAT
                     QKSPRSRRNAG"
BASE COUNT          136 a           93 c          110 g          120 t
ORIGIN      
        1 tcatgaaggt ttgtttaggt ttataaaaga tactcgtacg agtatctttt gcgagaggtt
       61 aatactcaaa tggttatctt gtcaatagac tatgcaaaaa agctacgtca aatacgcaag
      121 gctgaagggc taacgcaaaa ggaatttgcg gatattacgg ggttgtcttt agctgccata
      181 aggaactatg aatcagggca aaaaagcgcc agagccaaaa ttgttgagat ggttctccaa
      241 gttgatcggt ttgaaaaata catgatgtgg ctaataaaag ataagacaat gcctgctgcc
      301 ggtcagatag ctccggctct ctctcttgat ggctccattc agtcggagga cgatcaggat
      361 tcaatcccag ccacccaaaa atcaccccga tctcgtcgca atgctggcta acgctgtaca
      421 tcgagcgggt ttcattccgt agagagtgta cggctgatt
//
LOCUS       DAZFQD010000415          459 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.422, whole genome shotgun sequence.
ACCESSION   DAZFQD010000415 DAZFQD010000000
VERSION     DAZFQD010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..459
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.422"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..451
                     /gene="espX1"
                     /locus_tag="ACHKTW_005434"
     CDS             <1..451
                     /gene="espX1"
                     /locus_tag="ACHKTW_005434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system effector EspX1"
                     /protein_id="HFO6466249.1"
                     /translation="YFSCVLSNISCFESDLSNTIFSNGEINNLFIKKSNIFGASFTNT
                     RIKNLLCEDIMPGRWTTQLVNKHLGYRYTGVFKTLASIDDKPSRFEILIPLVQTLVRD
                     NVKLNNDVYKELKKFMHDYDKTSPEMRKYLQSINESMFLMKNIAHQN"
BASE COUNT          173 a           83 c           69 g          134 t
ORIGIN      
        1 ttattttagt tgtgtattat ccaatatatc atgttttgaa agcgacttat caaacaccat
       61 cttttcaaat ggagaaatta ataatctctt cattaaaaaa agcaacatat ttggagcatc
      121 attcactaat accagaataa aaaatttgct ctgtgaagat attatgcctg gtcgatggac
      181 cactcaactt gtcaataagc acttaggtta cagatacaca ggagtattca aaaccctggc
      241 aagtattgat gacaaaccgt cccgtttcga aattttgata ccattggtac aaactttagt
      301 tcgcgacaat gtaaagctaa ataatgatgt gtataaagaa ctgaaaaagt ttatgcacga
      361 ttacgacaaa acatccccgg aaatgagaaa ataccttcag agcatcaacg agtctatgtt
      421 ccttatgaaa aacatagctc atcaaaatta aaaaatctc
//
LOCUS       DAZFQD010000416          442 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.423, whole genome shotgun sequence.
ACCESSION   DAZFQD010000416 DAZFQD010000000
VERSION     DAZFQD010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..442
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.423"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
BASE COUNT          128 a           95 c           79 g          140 t
ORIGIN      
        1 tcacgtatgt aagtaatata tgaaaaggct ctcgttaaga tgtctggtac aaaatgacag
       61 atcatatgcc ttttgtaaag agacgacaga agttgagtct tacaggcaca tcataaatac
      121 cctccggcca tgccggaggg tatttattta actgccgctg tttgcactgg atgtggaact
      181 actcatacta taagaaggtc cacttccccc cggatacatg atgatggatg gagtacccgg
      241 agcccagcat gctccattag catcaatata tgttcccata tcttttcctc gtagatctat
      301 tacattagta gaactaggta atccattaag attcatccct cctttcacga aatcaagcat
      361 gtgatctgat atttctctca ttttaaaaac ctcctgctca tttatgttca gatattaatt
      421 tggtgttgca accatttatt at
//
LOCUS       DAZFQD010000417          436 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.424, whole genome shotgun sequence.
ACCESSION   DAZFQD010000417 DAZFQD010000000
VERSION     DAZFQD010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..436
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.424"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(96..305)
                     /locus_tag="ACHKTW_005435"
     CDS             complement(96..305)
                     /locus_tag="ACHKTW_005435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4035 domain-containing protein"
                     /protein_id="HFO6466250.1"
                     /translation="MWMEFDRVSPLGDERGDIRNAQIVKAVFGAQGMNVALKDAMLCW
                     GEDEDKPEVDPFAALEDALSFAAQS"
BASE COUNT           92 a          146 c           98 g          100 t
ORIGIN      
        1 ctgtgaattt gccgaaattt tgataatcag ttcacgtaac gtcgccattc acctttctcc
       61 gggcgtaaaa aaaccgcctc agcggttctc atcattcatg actgtgctgc aaagctcagc
      121 gcgtcttcca gcgccgcaaa cggatccacc tccggcttat cctcatcctc gccccagcag
      181 agcatggcgt ccttcagtgc aacattcatc ccctgtgccc cgaaaaccgc tttcacgatc
      241 tgtgcattac ggatatcccc gcgctcatca cccagcgggg ataccctgtc gaactccatc
      301 cacatcatcg cctcgctcgc actcaggctg tggcgcagtt cggataaggt gcgccccaga
      361 cggagcgcaa gtcgcatcag aaagcgaatt tccgggcggg ctactttttt ctggccgact
      421 ctgcatcagc gatcag
//
LOCUS       DAZFQD010000418          426 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.425, whole genome shotgun sequence.
ACCESSION   DAZFQD010000418 DAZFQD010000000
VERSION     DAZFQD010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..426
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.425"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>426
                     /locus_tag="ACHKTW_005436"
     CDS             <1..>426
                     /locus_tag="ACHKTW_005436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HFO6466251.1"
                     /translation="LSRKPQVTWYGWDGDRLTTIQNDRSRIQTIYQPGSFTPLIRVET
                     ATGELARTQRRSLADALQQSGGEDGGSVVFPPVLVQMLDRLESEILADRVSEESRRWL
                     ASCGLTVEQMQNQMDPVYTPARKIHLYHCDHRGLPLALI"
BASE COUNT           92 a          129 c          141 g           64 t
ORIGIN      
        1 gctgtcacgg aaaccgcaag tgacctggta cggctgggac ggcgaccggc tgaccacgat
       61 acagaacgac aggagccgca tccagacgat ttatcagccg gggagcttca caccactcat
      121 cagagttgaa actgccaccg gtgaactggc cagaacgcag cgccgcagcc tggcggatgc
      181 gcttcagcag tccggcggcg aagacggtgg cagtgtggtg ttcccgccgg tgctggtgca
      241 gatgctcgac cggctggaaa gtgaaatcct ggctgaccgg gtgagtgagg aaagccgccg
      301 ctggctggca tcgtgcggcc tgaccgtgga gcagatgcaa aaccagatgg acccggtgta
      361 cacgccggcg cgaaaaatcc acctgtacca ctgcgaccat cgcggcctgc cgctggcgct
      421 catcag
//
LOCUS       DAZFQD010000419          421 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.426, whole genome shotgun sequence.
ACCESSION   DAZFQD010000419 DAZFQD010000000
VERSION     DAZFQD010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..421
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.426"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>421
                     /locus_tag="ACHKTW_005437"
     CDS             <1..>421
                     /locus_tag="ACHKTW_005437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HFO6466252.1"
                     /translation="AITPYTLMQAIAAEGDVTTPVVVKPSDGEGTVISTTSEPEYYFV
                     VVLAGQSNSMSFGEGLPLPETYDRPDPRIKQLARRSTVTPGGAACKYNDIIPADHCLH
                     DVQDMSNLNHPHADLSKGQYGCVGHALHVAKKLLPFMP"
BASE COUNT           93 a          106 c          125 g           97 t
ORIGIN      
        1 ggccatcacg ccttataccc tgatgcaggc cattgcggcg gaaggtgatg tcaccacacc
       61 tgtggtggtg aagccgtcgg atggagaagg cacagttatc agcaccacca gcgaaccgga
      121 gtattacttt gttgttgttc tggcggggca gtcaaactca atgtcttttg gtgaagggct
      181 gccgctgccg gagacatatg accgtccgga cccgcgtatt aaacagctgg cgcgtcgcag
      241 tacggtgacg ccgggtggtg ccgcctgtaa atataacgac atcattccgg cagaccactg
      301 tctgcatgat gtgcaggata tgagtaacct gaatcatcct catgcagacc tgagtaaggg
      361 acagtacggt tgtgtggggc atgctctgca tgttgccaaa aagctgctgc cgtttatgcc
      421 t
//
LOCUS       DAZFQD010000420          404 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.427, whole genome shotgun sequence.
ACCESSION   DAZFQD010000420 DAZFQD010000000
VERSION     DAZFQD010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..404
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.427"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>404
                     /locus_tag="ACHKTW_005438"
     CDS             <1..>404
                     /locus_tag="ACHKTW_005438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6466253.1"
                     /translation="INVNFPLKDIYNEIRRLNLVFSCGTGGIVDLSSLDLRNIDLELY
                     DFTDKHMANAILNPFKLDDTDFTNANMFQVNFVSSKQNTTISWDYLLKITPVLTSISD
                     MYSEEKIKLVESCLNELGDITEEQLKIMRFAI"
BASE COUNT          143 a           59 c           68 g          134 t
ORIGIN      
        1 atcaatgtta atttcccatt gaaagacatt tacaatgaaa tcaggaggtt gaacttagtt
       61 tttagttgtg ggactggagg tatagttgat ctatcctctc tggacttacg gaatatcgat
      121 ttagaacttt atgattttac agataaacat atggccaatg caattttaaa tccttttaaa
      181 ttggatgaca cagattttac taatgccaac atgtttcagg taaatttcgt tagttcaaaa
      241 caaaacacca caatctcctg ggattattta ctaaaaataa cgcctgttct aacaagcatt
      301 agcgatatgt attctgaaga aaaaattaag cttgtcgaaa gttgtttaaa tgagcttgga
      361 gatattaccg aagaacaatt aaaaatcatg agatttgcaa ttat
//
LOCUS       DAZFQD010000421          404 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.428, whole genome shotgun sequence.
ACCESSION   DAZFQD010000421 DAZFQD010000000
VERSION     DAZFQD010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..404
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.428"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>404
                     /locus_tag="ACHKTW_005439"
     CDS             <1..>404
                     /locus_tag="ACHKTW_005439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HFO6466254.1"
                     /translation="RNVEAGVDKQTGEVREMPVTTYSDGTRTMTPDVGWSYNPGSAAF
                     GTDQALIRKLIEVKSPALREMVVQEMNNSPERQLAFRIWAKNIMKTRRGGNDIRTLGF
                     MTESIAQAVESRTGTPPARLLAMSGKNVLHAD"
BASE COUNT          104 a          102 c          134 g           64 t
ORIGIN      
        1 cgcaatgttg aggctggcgt ggataagcag accggggaag tcagagaaat gccggtgacc
       61 acatattcag atggcaccag aaccatgaca ccggatgtgg gctggtcata taaccccggt
      121 tcggcggcgt tcggcacaga ccaggcgctg atccgtaaac tgatcgaggt gaaaagcccg
      181 gcgttacggg aaatggtggt tcaggagatg aacaacagcc cggagcggca gctggcgttc
      241 cgcatctggg caaaaaacat catgaaaacc cggcgaggcg gtaacgatat ccgcacgctg
      301 ggctttatga ccgaaagtat tgcgcaggcg gtggaaagcc ggacgggaac gccaccggcc
      361 cgcctgctgg cgatgagcgg taaaaatgtg ctccatgcgg acag
//
LOCUS       DAZFQD010000422          404 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.429, whole genome shotgun sequence.
ACCESSION   DAZFQD010000422 DAZFQD010000000
VERSION     DAZFQD010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..404
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.429"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>404
                     /locus_tag="ACHKTW_005440"
     CDS             <1..>404
                     /locus_tag="ACHKTW_005440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO6466255.1"
                     /translation="GQQMTVNYHIRGRIIQVPSNYDPEKRTYSGIWDGSLKPAYSNNP
                     AWCLWDMLTHPRYGMGKRLGAADVDKWALYAIGQYCDQTVPDGFGGTEPRMTFNAYLA
                     QQRKVWDVLSDFCSAMRCMPVWNGQTLTFVQDR"
BASE COUNT           87 a          108 c          133 g           76 t
ORIGIN      
        1 ggccagcaga tgacggtgaa ctaccatatc cgcggtcgca tcatccaggt gccgtcaaac
       61 tatgacccgg aaaaacgcac gtacagtggt atctgggacg gcagtctgaa accggcatac
      121 agcaacaacc cggcctggtg tctgtgggac atgctgactc acccgcgcta cggcatggga
      181 aaacgtctgg gggcggcgga tgtggacaag tgggcgctgt atgccatcgg gcagtactgc
      241 gaccagacgg tcccggatgg tttcgggggc acagagccgc ggatgacctt taatgcgtac
      301 ctggcacaac agcgtaaggt gtgggacgtt ctcagtgatt tctgctcggc gatgcgctgt
      361 atgccggtat ggaacggcca gacgctgacg ttcgttcagg accg
//
LOCUS       DAZFQD010000423          398 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.430, whole genome shotgun sequence.
ACCESSION   DAZFQD010000423 DAZFQD010000000
VERSION     DAZFQD010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..398
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.430"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>398
                     /locus_tag="ACHKTW_005441"
     CDS             <1..>398
                     /locus_tag="ACHKTW_005441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001367836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFO6466256.1"
                     /translation="GDIVCFPDDDCIYTQSLLKNIYELFAYNNHVDFISVNTKDPIAG
                     EKALVKLPKSKTNISFKSKNCVSFTLFFRREVINAVGRFDQNMGVGAGTNYGAGEESD
                     FVVRALSLGFKGLYYPDYYVLHPAKESHPV"
BASE COUNT          126 a           63 c           82 g          127 t
ORIGIN      
        1 caggcgatat tgtatgtttt ccagatgatg actgcattta tacacagagc ctactaaaaa
       61 atatttatga gttatttgca tataataatc atgttgattt tatcagcgtt aatactaagg
      121 atcccattgc aggggaaaaa gcactggtca agctacctaa aagcaagact aatatttcat
      181 tcaagagtaa aaattgtgtt tcatttacat tatttttcag gcgcgaagtt ataaatgctg
      241 ttggtagatt tgatcaaaat atgggggtcg gagctggcac taactatggt gcaggagaag
      301 aaagcgactt tgtagttcgg gccctttctt taggttttaa aggtctgtac tatcctgact
      361 attatgtatt acatccagcg aaagaaagcc atccagta
//
LOCUS       DAZFQD010000424          389 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.431, whole genome shotgun sequence.
ACCESSION   DAZFQD010000424 DAZFQD010000000
VERSION     DAZFQD010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..389
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.431"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..386
                     /locus_tag="ACHKTW_005442"
     CDS             <1..386
                     /locus_tag="ACHKTW_005442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000406625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysostaphin resistance A-like protein"
                     /protein_id="HFO6466257.1"
                     /translation="LAVRKTEEWMISQMSFSGAILWINIFSSVLLVPVYEEIVFRGCL
                     FNSFKFWFNDNIYISAIVTSVIFSALHLQYTDFRTFLMLFLISLVLISAKIKSNGLLM
                     PILLHMAMNAVITGIQYTLQYHITV"
BASE COUNT          112 a           67 c           66 g          144 t
ORIGIN      
        1 tgctggcagt gaggaaaacg gaagaatgga tgatctcgca aatgtctttt tccggggcca
       61 ttttatggat taatatattt tcttccgtac ttcttgttcc tgtatatgaa gagattgtat
      121 ttcgagggtg tctttttaac tcttttaaat tctggtttaa tgacaatatt tacatctctg
      181 ctattgtaac atcagtcata ttttctgcat tacatctaca atatactgac tttcgtacat
      241 ttttgatgct ctttctgata tcgttagttc ttatcagtgc aaaaataaag tcgaatggcc
      301 tgttaatgcc catattactt cacatggcga tgaatgcagt cataacagga atacaatata
      361 cgttacaata tcacataaca gtctgaaat
//
LOCUS       DAZFQD010000425          387 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.432, whole genome shotgun sequence.
ACCESSION   DAZFQD010000425 DAZFQD010000000
VERSION     DAZFQD010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..387
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.432"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>387
                     /locus_tag="ACHKTW_005443"
     CDS             <1..>387
                     /locus_tag="ACHKTW_005443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFO6466258.1"
                     /translation="LKRALNIKLTLREYADHQKAMDALEEGEVDIVLSHLVTSPPLNN
                     DIAATKPLIITFPALVTTLHDSMRPLTSPKPVNIARVANYPPDEVIHQSFPKATIISF
                     TNLYQALASVSAGHNDYFIGSNIITS"
BASE COUNT          127 a           93 c           64 g          103 t
ORIGIN      
        1 tttaaaaaga gcgttaaata tcaaattaac actccgggaa tacgcagatc atcaaaaagc
       61 aatggacgcg ctggaagaag gtgaagtcga tatagtgtta tcacatttag ttacttcgcc
      121 gcctcttaat aatgacattg ctgcaaccaa accattgata attacctttc cggcgctggt
      181 aaccaccctt cacgactcaa tgcgaccgct tacctcacca aaaccagtaa atattgctcg
      241 ggtagcaaat taccccccag acgaggtaat tcatcaatca tttccaaaag caacaattat
      301 ctcttttaca aatttatatc aggcattagc atccgtctca gctgggcaca atgattactt
      361 tattggtagt aacatcatta ccagcag
//
LOCUS       DAZFQD010000426          380 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.433, whole genome shotgun sequence.
ACCESSION   DAZFQD010000426 DAZFQD010000000
VERSION     DAZFQD010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..380
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.433"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>380
                     /locus_tag="ACHKTW_005444"
     CDS             <1..>380
                     /locus_tag="ACHKTW_005444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO6466259.1"
                     /translation="DPEVLKQAVNDAVANIPVPTDGKSITPDDVRPMLEQMVKEAVSH
                     IPAPRDGCDYDPDVLRKAVLEAVNALPAPQDGRDATALEILPAIDDQKSFPRGTYATH
                     QGGLWRAYEKTHGMRGWECLVDGVA"
BASE COUNT           83 a           94 c          129 g           74 t
ORIGIN      
        1 gatccggaag tactgaagca ggcggtgaat gatgcggttg cgaatattcc ggtaccgacg
       61 gacggcaaaa gtattacccc cgatgatgtg cgtccgatgc ttgagcagat ggtgaaagag
      121 gccgtaagtc atatccctgc tccgcgtgac ggttgtgact atgatccgga tgttctgcgg
      181 aaggcggttc tggaggcggt gaatgccctg ccggctccgc aggacgggcg tgatgccacg
      241 gcactggaaa tactccctgc cattgacgat caaaaatcct ttccccgggg cacgtatgcc
      301 acacaccagg gcggactctg gcgggcgtat gaaaaaacgc acgggatgcg gggatgggaa
      361 tgcctggttg acggggtggc
//
LOCUS       DAZFQD010000427          374 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.434, whole genome shotgun sequence.
ACCESSION   DAZFQD010000427 DAZFQD010000000
VERSION     DAZFQD010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..374
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.434"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>374
                     /locus_tag="ACHKTW_005445"
     CDS             <1..>374
                     /locus_tag="ACHKTW_005445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6466260.1"
                     /translation="DKHSDKVNSHQHHGKVDKTHRAKIVEFDKLDNDSQIDNDFGLHI
                     IYFLQHGHWKVNDRSHQMEKVWFYNSEPSIDIQEYNRFADNTTDTFIFTIIPDNNHVL
                     KLSSPITVTVECKGGYYFINSS"
BASE COUNT          128 a           62 c           66 g          118 t
ORIGIN      
        1 agataagcat tcagataaag ttaacagtca tcaacaccat ggcaaggttg ataaaacaca
       61 tcgtgcaaag atagttgaat ttgataaact ggataatgat tcgcaaatag ataacgattt
      121 tggcctgcat atcatctatt tcctgcaaca tggtcactgg aaggtaaatg atcgtagtca
      181 ccagatggag aaagtatggt tttataatag tgaaccttct atagatattc aagaatataa
      241 tagattcgcc gacaatacta ctgatacgtt tatctttaca attataccag acaataacca
      301 tgtgttaaaa ttatcgtctc ccattaccgt tactgttgag tgtaagggcg gttattattt
      361 tattaattcc tctg
//
LOCUS       DAZFQD010000428          367 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.435, whole genome shotgun sequence.
ACCESSION   DAZFQD010000428 DAZFQD010000000
VERSION     DAZFQD010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..367
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.435"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            21..>367
                     /locus_tag="ACHKTW_005446"
     CDS             21..>367
                     /locus_tag="ACHKTW_005446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6466261.1"
                     /translation="MIIDLDTLFPVRKTFTDIVSYCTDPFASVENKVFASLPADVECG
                     DLITSTGAKYESGDDIYVVMSEFVTAGENKPVDVLRSNAGLVCIKADALNAVSEAAKT
                     ALIKKGFQLEGFHS"
BASE COUNT          108 a           63 c           84 g          112 t
ORIGIN      
        1 aaagctaagg agattactaa atgattattg atttagacac tctgtttcca gttcgaaaga
       61 ctttcacaga tattgtaagt tattgtactg atccgtttgc ctctgtagaa aacaaggttt
      121 ttgcttctct tcctgctgat gttgaatgcg gcgacttgat caccagcacg ggcgctaaat
      181 atgaatcagg agatgatatc tatgttgtta tgagtgaatt tgtaacggct ggagaaaaca
      241 agcctgtaga tgttttacgt agcaatgctg ggcttgtctg catcaaagct gatgcgctga
      301 atgctgtaag tgaagcagca aaaacagcat taattaaaaa aggcttccag cttgaagggt
      361 tccattc
//
LOCUS       DAZFQD010000429          366 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.436, whole genome shotgun sequence.
ACCESSION   DAZFQD010000429 DAZFQD010000000
VERSION     DAZFQD010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..366
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.436"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..278)
                     /locus_tag="ACHKTW_005447"
     CDS             complement(<1..278)
                     /locus_tag="ACHKTW_005447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HFO6466262.1"
                     /translation="MTSKWVQLSSMPGNFTVKVSGGTAAFLEAPFPPAETKGGMTFAD
                     CLISFNTRDCLWVRPVSGDPNVEITGAGIGAVIPLSADVAGTTEPSDW"
BASE COUNT          109 a           95 c           73 g           89 t
ORIGIN      
        1 ttccagtctg acggttcagt agtaccggca acatcagcac ttaacggaat gacagcacca
       61 atacccgctc cggtaatttc cacgttcgga tcaccggata ctggccttac ccacaggcaa
      121 tcccgtgtat taaaactgat gagacagtca gcaaatgtca tacctccttt cgtttcagcc
      181 ggaggaaagg gagcctcaag aaatgccgct gtaccaccgg aaactttaac ggtaaagttt
      241 ccgggcattg atgatagttg aacccatttg gacgtcataa tattcatcct gttcctgaat
      301 aaacagaatt atcatatcca cagaccgata aacgccattt tgcgttaatt cagtaaaggc
      361 ccaaca
//
LOCUS       DAZFQD010000430          356 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.437, whole genome shotgun sequence.
ACCESSION   DAZFQD010000430 DAZFQD010000000
VERSION     DAZFQD010000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..356
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.437"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>356
                     /locus_tag="ACHKTW_005448"
     CDS             <1..>356
                     /locus_tag="ACHKTW_005448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HFO6466263.1"
                     /translation="EYPQSAAIDLRCVNMVADLWHAPAPKNGQAVGTNTIGSSEACML
                     GGMAMKWRWRKRMEAAGKPTDKPNLVCGPVQICWHKFARYWDVELREIPMRPGQLFMD
                     PKRMIEACDENTIGVVP"
BASE COUNT           81 a           94 c          106 g           75 t
ORIGIN      
        1 gaatatccgc aatccgcagc catcgacctg cgttgcgtaa atatggttgc cgatctgtgg
       61 catgcgcctg cgccgaaaaa tggtcaggcc gttggcacca acaccattgg ttcttccgag
      121 gcctgtatgc tcggcgggat ggcgatgaaa tggcgttggc gcaagcgtat ggaagctgca
      181 ggcaaaccaa cggataaacc aaacctggtg tgcggtccgg tacaaatctg ctggcataaa
      241 ttcgcccgct actgggatgt ggagctgcgt gagatcccta tgcgccccgg tcagttgttt
      301 atggacccga aacgcatgat tgaagcctgt gacgaaaaca ccatcggcgt ggtgcc
//
LOCUS       DAZFQD010000431          355 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.438, whole genome shotgun sequence.
ACCESSION   DAZFQD010000431 DAZFQD010000000
VERSION     DAZFQD010000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..355
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.438"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            157..232
                     /locus_tag="ACHKTW_005449"
     tRNA            157..232
                     /locus_tag="ACHKTW_005449"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:191..193,aa:Glu,seq:ttc)
BASE COUNT          102 a           81 c           84 g           88 t
ORIGIN      
        1 ctttgcagtg ctcacacaga ttgtctgata gaaagtgaaa agcaaggcgt ttacgcgttg
       61 ggagtgaggc tgaagagaat aaggccgttc gctttctatt aatgaaagct caccctacac
      121 gaaaatatca cgcaacgcgt gataagcaat tttcgtgtcc ccttcgtcta gaggcccagg
      181 acaccgccct ttcacggcgg taacaggggt tcgaatcccc taggggacgc cacttgctgg
      241 tttgtgagtg aaagtcgccg accccaatat ctcaaaacag actgttaagt cttgtttgag
      301 atatttgctc tttaaaaatc tggatcaagc tgaaaattga aacactgaac aacga
//
LOCUS       DAZFQD010000432          347 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.439, whole genome shotgun sequence.
ACCESSION   DAZFQD010000432 DAZFQD010000000
VERSION     DAZFQD010000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..347
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.439"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..290
                     /locus_tag="ACHKTW_005450"
     CDS             <1..290
                     /locus_tag="ACHKTW_005450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6466264.1"
                     /translation="YQASNFDYVGSHETIFYELDGEWYHEICRNAIKRGGQRGEHLRA
                     NIDRASVHKFRQFRYIRFLNKRARKRLNTKLFKVQPYPKPQTVKTGLKESE"
BASE COUNT          125 a           69 c           75 g           78 t
ORIGIN      
        1 tgtatcaggc aagcaatttt gattatgtag gcagccatga aacaatattc tatgaacttg
       61 atggcgagtg gtatcacgag atttgcagga atgccattaa acgaggcgga caacgaggtg
      121 aacatctgag ggctaatatc gacagagcca gtgtgcataa gttccgacag tttcgttaca
      181 tccggtttct gaacaagagg gccagaaaac gcctcaacac aaagctattc aaagtccagc
      241 cttacccaaa accacaaaca gttaaaactg gtttgaaaga gagtgaatga gcgcttaaaa
      301 actcattcta aaactcaaaa gaaaagcgcc gatttagtat caaataa
//
LOCUS       DAZFQD010000433          343 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.440, whole genome shotgun sequence.
ACCESSION   DAZFQD010000433 DAZFQD010000000
VERSION     DAZFQD010000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..343
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.440"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            18..>343
                     /locus_tag="ACHKTW_005451"
     CDS             18..>343
                     /locus_tag="ACHKTW_005451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage protein Gp27 family protein"
                     /protein_id="HFO6466265.1"
                     /translation="MDKPTRGRVRKVDLLPDSIRKPLLEMLREKRLTQVQIREEINRL
                     IREAGLPEEQQLSPAAISREASRNELIARNLRDLREQTKAMMAELGDKPTGETTALIL
                     EMSRAL"
BASE COUNT           78 a           97 c          111 g           57 t
ORIGIN      
        1 cgcgcaaggg ggtaacgatg gacaagccga cgcgtggccg cgtgcgcaag gtggatttgc
       61 tccccgacag catccgtaag ccgctgctgg aaatgctgcg tgaaaaacgt ctgacgcagg
      121 tccagatccg cgaggaaatc aaccgtctga tccgcgaggc aggcctgccg gaagagcagc
      181 agctttcacc ggcggccatc agccgggaag cctcccgtaa tgagctgatt gcccgcaatc
      241 tgcgtgattt gcgcgagcag acaaaagcca tgatggctga actgggggat aagccgaccg
      301 gtgaaactac ggcgctgatt ctggagatgt cccgtgccct gat
//
LOCUS       DAZFQD010000434          342 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.441, whole genome shotgun sequence.
ACCESSION   DAZFQD010000434 DAZFQD010000000
VERSION     DAZFQD010000434.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..342
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.441"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
BASE COUNT          105 a           67 c           65 g          105 t
ORIGIN      
        1 aaaaatcccc gttcccagag aaataatatt tattaaaatt ccagttcttc tttttctgat
       61 tacagaaggc aaagtggcaa taacgcatag tttcccgata aagacgcgat agcgacatcc
      121 cgcataaggc atttttctct ttatctttgt acggtacttc atggaacaga gtttttgacc
      181 ttgcgaatcg tgatgtctgt tgggggcaac ttgctcactg aagcgtgaga ctcgattaag
      241 cgcacgaaac acagaaatca aaaaacccgg tcactttttt acaaggtaac cgggtaaaaa
      301 taatttttat tttttaactg ttttgagact catagagatg tc
//
LOCUS       DAZFQD010000435          340 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.442, whole genome shotgun sequence.
ACCESSION   DAZFQD010000435 DAZFQD010000000
VERSION     DAZFQD010000435.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..340
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.442"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..147
                     /locus_tag="ACHKTW_005452"
     CDS             <1..147
                     /locus_tag="ACHKTW_005452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HFO6466266.1"
                     /translation="KGNVSIDGTGRSQGTFTMSEAVIAGITYSGHVHHDNGEGSKTGG
                     PENG"
     gene            140..>340
                     /locus_tag="ACHKTW_005453"
     CDS             140..>340
                     /locus_tag="ACHKTW_005453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage GP46 family protein"
                     /protein_id="HFO6466267.1"
                     /translation="MADIAVVWDQGCGSLQLNGADLLTDNSLLTAVIISLFTDRRALD
                     SDEIPDGTRDRRGWWGDSFRERP"
BASE COUNT           77 a           82 c          109 g           72 t
ORIGIN      
        1 aaaggcaatg tgtccattga cggcaccgga agatcacagg ggacgttcac gatgtccgaa
       61 gcggttattg ccgggatcac ctattcaggt catgtgcatc acgataacgg tgaaggcagc
      121 aaaacgggag gaccagagaa tggctgatat tgctgtcgtc tgggatcagg gttgtggttc
      181 cctgcaactg aacggcgcag atcttctgac ggataacagc ctgctgactg cggtcattat
      241 ttcactgttt acggacaggc gggcgctgga ttccgatgaa atccctgacg gcacccgtga
      301 ccgtcgggga tggtggggag acagtttccg ggagcgcccc
//
LOCUS       DAZFQD010000436          340 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.443, whole genome shotgun sequence.
ACCESSION   DAZFQD010000436 DAZFQD010000000
VERSION     DAZFQD010000436.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..340
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.443"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            9..>340
                     /locus_tag="ACHKTW_005454"
     CDS             9..>340
                     /locus_tag="ACHKTW_005454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu-like prophage major head subunit gpT family
                     protein"
                     /protein_id="HFO6466268.1"
                     /translation="MPTPITPAMITALMTGYRSDFQAGMSMAPSQYKKIAMTVPSTSK
                     SNTYGWLGQFPQFREWIGSRVIEKMKAYGYAIVNKTFEGTVAINRDDFEDDNLGIYSP
                     LFQEMGRAA"
BASE COUNT           77 a          102 c           88 g           73 t
ORIGIN      
        1 atgcaataat gcctaccccg attacaccgg cgatgatcac cgccctgatg acgggctacc
       61 gttctgattt tcaggccggg atgtccatgg ccccgtcgca gtacaaaaaa attgcgatga
      121 ccgtgccctc cacctcgaaa tccaacacct acggctggct ggggcagttc ccgcagttcc
      181 gtgagtggat cggctcccgc gtcatcgaga agatgaaggc ctatggctat gccatcgtga
      241 acaaaacctt tgaaggcacc gtcgccatta accgcgatga cttcgaagac gataacctcg
      301 gtatttattc cccgttgttc caggagatgg ggcgcgctgc
//
LOCUS       DAZFQD010000437          340 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.444, whole genome shotgun sequence.
ACCESSION   DAZFQD010000437 DAZFQD010000000
VERSION     DAZFQD010000437.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..340
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.444"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..201)
                     /locus_tag="ACHKTW_005455"
     CDS             complement(<1..201)
                     /locus_tag="ACHKTW_005455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage GP46 family protein"
                     /protein_id="HFO6466269.1"
                     /translation="MADIAIVWDQGCGSLQLNGADLLTDDSLLTAVLISLFTDRRALD
                     SDEIPDGTRDRRGWWGDSFRERP"
     gene            complement(194..>340)
                     /locus_tag="ACHKTW_005456"
     CDS             complement(194..>340)
                     /locus_tag="ACHKTW_005456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HFO6466270.1"
                     /translation="KGNFALDGTGKSQGLFTMSDAVIAGIRYSGHVHQDNGKGSKTGA
                     PENG"
BASE COUNT           74 a          118 c           74 g           74 t
ORIGIN      
        1 ggggcgctcc cggaaactgt ctccccacca tccccgcctg tcacgagtgc cgtcagggat
       61 ttcatcagaa tccagcgccc gcctgtccgt aaacagcgaa atcagaacgg ccgtcagcag
      121 actgtcatcc gtcagcaggt cggcaccgtt cagttgcagc gagccgcacc cctgatccca
      181 tacaattgca atatcagcca ttctccggtg ctcctgtctt actgcctttg ccgttatcct
      241 gatgcacatg accggaatac cgtatccctg caataacggc atccgacatt gtgaacagtc
      301 cctgagattt ccccgtacca tcaagcgcga aattcccctt
//
LOCUS       DAZFQD010000438          336 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.445, whole genome shotgun sequence.
ACCESSION   DAZFQD010000438 DAZFQD010000000
VERSION     DAZFQD010000438.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..336
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.445"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(138..336)
                     /locus_tag="ACHKTW_005457"
                     /pseudo
     CDS             complement(138..336)
                     /locus_tag="ACHKTW_005457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
BASE COUNT          113 a           66 c           54 g          103 t
ORIGIN      
        1 accttattta tgtaaggagt atttgatcat gtataagcta tgaaaaacag ctttagacaa
       61 taagtagtat ctcaattgtt gaacttaaaa ttcgaattat ttagtacaaa aaagtagcgt
      121 tacgtgatct ttccctggga tacaactctg gcaatggtat cgtgaacagg aataccattt
      181 tcaaaatcac catattgctt caaaaaatcg agatgtgttt ccccgaaatc ctctatatct
      241 tcccaacctt ctgcaccaga aataacggca caaatagtca acagtagaat atctgacaat
      301 ttatgcacca ctttccaggc ttgtctgtaa tcgggt
//
LOCUS       DAZFQD010000439          336 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.446, whole genome shotgun sequence.
ACCESSION   DAZFQD010000439 DAZFQD010000000
VERSION     DAZFQD010000439.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..336
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.446"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..242)
                     /locus_tag="ACHKTW_005458"
     CDS             complement(<1..242)
                     /locus_tag="ACHKTW_005458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HFO6466271.1"
                     /translation="MDKKQVTDLRSELLDSRFGAKSISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSI"
BASE COUNT          104 a           63 c           70 g           99 t
ORIGIN      
        1 ttaatggata aatccatcaa tttgtggaca ttttcgtcgt cccaggtctg gcagaaagtg
       61 gccaggttct gacgagcgtt gccatcaaga tataattcgt cattgataat ctggaatgcg
      121 acatcgtcgc gcatttcgtg cagcggaaaa cgttttgatt ctgcgatagt ggaaatagac
      181 ttcgcaccaa aacgtgaatc gagtagttcc gaccttaaat ccgttacttg cttcttatcc
      241 attttaaact ccttaaaatg attggatcgc attaaaaaag taggatttat cgataaagta
      301 agcaagtgca aaggactcgt gtttaaataa caaaat
//
LOCUS       DAZFQD010000440          330 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.447, whole genome shotgun sequence.
ACCESSION   DAZFQD010000440 DAZFQD010000000
VERSION     DAZFQD010000440.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..330
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.447"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            80..286
                     /locus_tag="ACHKTW_005459"
     CDS             80..286
                     /locus_tag="ACHKTW_005459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO6466272.1"
                     /translation="MEAIRKIIFRQEVKKIIHIKADSTLQSMINAGEFPQGFRVGLRR
                     RGWYEDDVLSWQKEREEEARGTAA"
BASE COUNT          105 a           71 c           84 g           70 t
ORIGIN      
        1 gtgggaactg tttttaccca tatccgatga tttaccgtat ttgtcaccgg aatacaccgg
       61 attcacaagg taaatcacga tggaagcaat cagaaaaatt atctttcgcc aggaagtaaa
      121 aaaaattatc cacatcaagg cagacagcac gctgcaaagc atgatcaacg ccggagaatt
      181 tccgcagggt tttcgcgttg gtttacgccg tcgcggatgg tatgaggatg atgtgttgtc
      241 ctggcagaaa gaacgcgaag aggaagcacg cggaacggct gcttaacggg tatcacagat
      301 atgacaaaca cgaaaaaagc ggccccgata
//
LOCUS       DAZFQD010000441          328 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.448, whole genome shotgun sequence.
ACCESSION   DAZFQD010000441 DAZFQD010000000
VERSION     DAZFQD010000441.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..328
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.448"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(92..319)
                     /gene="mchB"
                     /locus_tag="ACHKTW_005460"
     CDS             complement(92..319)
                     /gene="mchB"
                     /locus_tag="ACHKTW_005460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001375214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin H47"
                     /protein_id="HFO6466273.1"
                     /translation="MREITESQLRYISGAGGAPATSANAAGAAAIVGALAGIPGGPLG
                     VVVGAVSAGLTTAIGSTVGSGSASSSAGGGS"
BASE COUNT          102 a           86 c           61 g           79 t
ORIGIN      
        1 ttataaatgg atcttctatc tgttttaaac aaaaaggata tgttttacag gtactaccga
       61 aaaagtgcct gtaaaataaa taaaaatata attagctacc gccaccagca gaagaactgg
      121 cactaccact tcccacggtc gagccaattg ctgttgtcaa accggcagat acggctccaa
      181 ctacaacccc aagtggacca ccaggtattc cggcgagagc tccaacaata gctgcagcac
      241 ctgcagcatt agctgaagtc gctggcgcac ctcccgcccc ggaaatatat cttaactgtg
      301 attctgttat ttctcgcata taaactgc
//
LOCUS       DAZFQD010000442          324 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.449, whole genome shotgun sequence.
ACCESSION   DAZFQD010000442 DAZFQD010000000
VERSION     DAZFQD010000442.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..324
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.449"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            194..>324
                     /locus_tag="ACHKTW_005461"
     CDS             194..>324
                     /locus_tag="ACHKTW_005461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310767.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6466274.1"
                     /translation="MEKYKRILLRLTMKNGLELKAPVTDDISRALAFAIKWVAVGIAV
                     "
BASE COUNT           96 a           56 c           97 g           75 t
ORIGIN      
        1 ggagagcgcg ctggcgtgct ggggaatcga cagataagaa gaatattttg ctgaaaaatg
       61 acgttggcca acgcgggcgg ataacacgaa atcctgcgaa ctggcaaaac ctaagtgaat
      121 aaaagtaaaa accccgtttg ttggcagcaa gcggggtttt gtgtttctga ccttggataa
      181 ggcaagggag aacatggaaa agtataaacg aattctgttg aggttgacta tgaaaaacgg
      241 ccttgaactg aaagcgcctg taactgatga catcagcaga gcgctggctt ttgctattaa
      301 gtgggtggcg gtcggtattg ctgt
//
LOCUS       DAZFQD010000443          310 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.451, whole genome shotgun sequence.
ACCESSION   DAZFQD010000443 DAZFQD010000000
VERSION     DAZFQD010000443.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..310
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.451"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..242)
                     /locus_tag="ACHKTW_005462"
     CDS             complement(<1..242)
                     /locus_tag="ACHKTW_005462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HFO6466275.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSI"
BASE COUNT           89 a           64 c           71 g           86 t
ORIGIN      
        1 ttgatcgaca aatccatcaa tttatggacg ttttcgtcgt cccaggtctg gcagaaagtg
       61 gccaggttct gacgagcgtt gccatcaaga tataattcat cattgataat ctggaatgcg
      121 acatcatcgc gcatttcgtg cagcggaaat cgttttgact ccgcgatagt agaaatggcc
      181 tttgcgccaa aacgtgaatc gagtagttct gagcggaaat ccgttaacag cttctggtcc
      241 atttcgaact ccttaaattt atttgaaggc aataaaaaag taggatttat ccgcaatgga
      301 agcaaggcat
//
LOCUS       DAZFQD010000444          309 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.452, whole genome shotgun sequence.
ACCESSION   DAZFQD010000444 DAZFQD010000000
VERSION     DAZFQD010000444.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..309
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.452"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..275)
                     /locus_tag="ACHKTW_005463"
     CDS             complement(<1..275)
                     /locus_tag="ACHKTW_005463"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO6466276.1"
                     /translation="MSDKLPSDYEILFDLWYSYWLEHMTSLLHRRASLVLRISAFLAI
                     VAAFIFCDSATFVFASVVLFLVWQVMAASLSIRAEKAEWQALSYQRL"
BASE COUNT          102 a           79 c           72 g           56 t
ORIGIN      
        1 atcagtcttt gatagctaag cgcctgccat tcagcctttt cagctctgat agaaagagag
       61 gcagccatga cctgccacac cagaaagagc accacactgg cgaaaacaaa cgttgcgctg
      121 tcgcaaaaaa tgaatgcggc aacaatcgca aggaaagcgc taatcctcag aaccaaggaa
      181 gcgcgacggt ggagaaggct tgtcatgtgt tcaagccagt agctgtacca caggtcaaaa
      241 agaatttcgt aatcagaagg gagcttatca ctcatcagaa acaccccgcg tatgcagaca
      301 atcaccagt
//
LOCUS       DAZFQD010000445          307 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.denovo.453, whole genome shotgun sequence.
ACCESSION   DAZFQD010000445 DAZFQD010000000
VERSION     DAZFQD010000445.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..307
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.denovo.453"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>307
                     /locus_tag="ACHKTW_005464"
     CDS             <1..>307
                     /locus_tag="ACHKTW_005464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HFO6466277.1"
                     /translation="VASSLLSSSEMAFEQEKEPDGERWHDWSDPYRKWRTRKGYMPGK
                     ILTLNGDLARRLTTDYGDTWALIGSNEPYAAIHQWGGLPGMPPGPAAIGARPYMGFDQ
                     "
BASE COUNT           65 a           83 c           96 g           63 t
ORIGIN      
        1 gtggcgtcca gccttctgtc atcctcagaa atggcgtttg aacaggaaaa agagccggac
       61 ggcgaacgct ggcatgactg gtcagatcct taccgcaagt ggcgtacccg taagggatac
      121 atgcccggca aaatcctgac gctgaacggc gatctggccc gacgcctgac cacggattat
      181 ggcgatacct gggcgctgat tggatcaaat gagccttatg cggccattca tcagtggggt
      241 ggcctgccag gtatgccgcc tggaccggcg gccattggtg cacgtccgta tatgggcttt
      301 gatcagg
//
LOCUS       DAZFQD010000446         4889 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.guided.223, whole genome shotgun sequence.
ACCESSION   DAZFQD010000446 DAZFQD010000000
VERSION     DAZFQD010000446.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4889
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.guided.223"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..260)
                     /locus_tag="ACHKTW_005465"
     CDS             complement(<1..260)
                     /locus_tag="ACHKTW_005465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3330 domain-containing protein"
                     /protein_id="HFO6466278.1"
                     /translation="MEVVAEGVETPDCLAWLRQAGCDTVQGFLFARPMPAAAFVGFVN
                     QWRNTTMNANEPSTSCCVCCKEIPLDAAFTPEGAEYVEHFCGL"
     gene            complement(463..1476)
                     /gene="intI1"
                     /locus_tag="ACHKTW_005466"
     CDS             complement(463..1476)
                     /gene="intI1"
                     /locus_tag="ACHKTW_005466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012077405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HFO6466279.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATP
                     HTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLT
                     SER"
     gene            1633..2106
                     /gene="dfrA1"
                     /locus_tag="ACHKTW_005467"
     CDS             1633..2106
                     /gene="dfrA1"
                     /locus_tag="ACHKTW_005467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA1"
                     /protein_id="HFO6466280.1"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVLIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISTIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
     gene            2224..2856
                     /locus_tag="ACHKTW_005468"
     CDS             2224..2856
                     /locus_tag="ACHKTW_005468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002075262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B-3 chloramphenicol O-acetyltransferase
                     CatB3"
                     /protein_id="HFO6466281.1"
                     /translation="MTNYFDSPFKGKLLSEQVKNPNIKVGRYSYYSGYYHGHSFDDCA
                     RYLFPDRDDVDKLIIGSFCSIGSGASFIMAGNQGHRYDWASSFPFFYMQEEPAFSSAL
                     DAFQKAGNTVIGNDVWIGSEAMVMPGIKIGHGAVIGSRSLVTKDVEPYAIVGGNPAKK
                     IKKRFTDEEISLLLEMEWWNWSLEKIKAAMPMLCSSNIVGLHKYWLEFAV"
     gene            2914..3702
                     /locus_tag="ACHKTW_005469"
     CDS             2914..3702
                     /locus_tag="ACHKTW_005469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA5"
                     /protein_id="HFO6466282.1"
                     /translation="MGEFFPAQVFKQLSHARAVIERHLAATLDTIHLFGSAIDGGLKP
                     DSDIDLLVTVSAAPNDSLRQALMLDLLKVSSPPGDGGTWRPLELTVVARSEVVPWRYP
                     ARRELQFGEWLRHDILSGTFEPAVLDHDLAILLTKARQHSLALLGPSAATFFEPVPKE
                     HFSKALFDTIAQWNAESDWKGDERNVVLALARIWYSASTGLIAPKDVAAAWVSERLPA
                     EHRPLICKARAAYLGSEDDDLAMRVEETAAFVRYAKATIERILR"
     gene            3908..4255
                     /locus_tag="ACHKTW_005470"
     CDS             3908..4255
                     /locus_tag="ACHKTW_005470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter QacE delta 1"
                     /protein_id="HFO6466283.1"
                     /translation="MKGWLFLVIAIVGEVIATSALKSSEGFTKLAPSAVVIIGYGIAF
                     YFLSLVLKSIPVGVAYAVWSGLGVVIITAIAWLLHGQKLDAWGFVGMGLIIAAFLLAR
                     SPSWKSLRRPTPW"
     gene            4249..>4889
                     /gene="folP"
                     /locus_tag="ACHKTW_005471"
     CDS             4249..>4889
                     /gene="folP"
                     /locus_tag="ACHKTW_005471"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019727244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HFO6466284.1"
                     /translation="MVTVFGILNLTEDSFFDESRRLDPAGAVTAAIEMLRVGSDVVDV
                     GPAASHPDARPVSPADEIRRIAPLLDALSDQMHRVSIDSFQPETQRYALKRGVGYLND
                     IQGFPDPALYPDIAEADCRLVVMHSAQRDGIATRTGHLRPEDALDEIVRFFEARVSAL
                     RRSGVAADRLILDPGMGFFLSPAPETSLHVLSNLQKLKSALGLPLLVSVSRKS"
BASE COUNT         1068 a         1344 c         1373 g         1104 t
ORIGIN      
        1 agcccgcaga aatgctccac gtactcggcc ccttccggcg tgaaggcggc atcgagcggg
       61 atttccttgc agcacacgca gcaactggtg ctcggttcat tggcgttcat ggtggtgttc
      121 ctccattggt tgacgaagcc gacgaaggcc gccgccggca tcggcctggc gaacaggaaa
      181 ccctgcaccg tgtcgcaacc cgcctgccgc aaccacgcaa ggcagtcggg tgtttccaca
      241 ccctcggcta ccacctccat tgtcgttttc agaagacggc tgcactgaac gtcagaagcc
      301 gactgcacta tagcagcgga ggggttggat ccatcaggca acgacgggct gctgccggcc
      361 atcagcggac gcagggagga ctttccgcaa ccggccgttc gatgcggcac cgatggcctt
      421 cgcgcagggg tagtgaatcc gccaggattg acttgcgctg ccctacctct cactagtgag
      481 gggcggcagc gcatcaagcg gtgagcgcac tccggcaccg ccaactttca gcacatgcgt
      541 gtaaatcatc gtcgtagaga cgtcggaatg gccgagcaga tcctgcacgg ttcgaatgtc
      601 gtaaccgctg cggagcaagg ccgtcgcgaa cgagtggcgg agggtgtgcg gtgtggcggg
      661 cttcgtgatg cctgcttgtt ctacggcacg tttgaaggcg cgctgaaagg tctggtcata
      721 catgtgatgg cgacgcacga caccgctccg tggatcggtc gaatgcgtgt gctgcgcaaa
      781 aacccagaac cacggccagg aatgcccggc gcgcggatac ttccgctcaa gggcgtcggg
      841 aagcgcaacg ccgctgcggc cctcggcctg gtccttcagc caccatgccc gtgcacgcga
      901 cagctgctcg cgcaggctgg gtgccaagct ctcgggtaac atcaaggccc gatccttgga
      961 gcccttgccc tcccgcacga tgatcgtgcc gtgatcgaaa tccagatcct tgacccgcag
     1021 ttgcaaaccc tcactgatcc gcatgcccgt tccatacaga agctgggcga acaaacgatg
     1081 ctcgccttcc agaaaaccga ggatgcgaac cacttcatcc ggggtcagca ccaccggcaa
     1141 gcgccgcgac ggccgaggtc ttccgatctc ctgaagccag ggcagatccg tgcacagcac
     1201 cttgccgtag aagaacagca aggccgccaa tgcctgacga tgcgtggaga ccgaaacctt
     1261 gcgctcgttc gccagccagg acagaaatgc ctcgacttcg ctgctgccca aggttgccgg
     1321 gtgacgcaca ccgtggaaac ggatgaaggc acgaacccag tggacataag cctgttcggt
     1381 tcgtaagctg taatgcaagt agcgtatgcg ctcacgcaac tggtccagaa ccttgaccga
     1441 acgcagcggt ggtaacggcg cagtggcggt tttcatggct tgttatgact gtttttttgt
     1501 acagtctatg cctcgggcat ccaagcagca agcgcgttac gccgtgggtc gatgtttgat
     1561 gttatggagc agcaacgatg ttacgcagca gggcagtcgc cctaaaacaa agttaacctc
     1621 tgaggaagaa ttgtgaaact atcactaatg gtagctatat cgaagaatgg agttatcggg
     1681 aatggccctg atattccatg gagtgccaaa ggtgaacagc tcctgtttaa agctattacc
     1741 tataaccaat ggctgttggt tggacgcaag acttttgaat caatgggagc attacccaac
     1801 cgaaagtatg cggtcgtaac acgttcaagt tttacatctg acaatgagaa cgtattgatc
     1861 tttccatcaa ttaaagatgc tttaaccaac ctaaagaaaa taacggatca tgtcattgtt
     1921 tcaggtggtg gggagatata caaaagcctg atcgatcaag tagatacact acatatatct
     1981 acaatagaca tcgagccgga aggtgatgtt tactttcctg aaatccccag caattttagg
     2041 ccagttttta cccaagactt cgcctctaac ataaattata gttaccaaat ctggcaaaag
     2101 ggttaacaag tggcagcaac ggattcgcaa acctgtcacg ccttttgtgc caaaagccgc
     2161 gccaggtttg cgatccgctg tgccaggcgt tagacggcaa agtcacagac cgcgggatct
     2221 cttatgacca actactttga tagccccttc aaaggcaagc tgctttctga gcaagtgaag
     2281 aaccccaata tcaaagttgg gcggtacagc tattactctg gctactatca tgggcactca
     2341 ttcgatgact gcgcacggta tctgtttccg gaccgtgatg acgttgataa gttgatcatc
     2401 ggtagtttct gctctatcgg gagtggggct tcctttatca tggctggcaa tcaggggcat
     2461 cggtacgact gggcatcatc tttcccgttc ttttatatgc aggaagaacc tgcattctca
     2521 agcgcactcg atgccttcca aaaagcaggt aatactgtca ttggcaatga cgtttggatc
     2581 ggctctgagg caatggtcat gcccggaatc aagatcgggc acggtgcggt gataggcagc
     2641 cgctcgttgg tgacaaaaga tgtggagcct tacgctatcg ttggcggcaa tcccgctaag
     2701 aagattaaga aacgcttcac cgatgaggaa atttcattgc ttctggagat ggagtggtgg
     2761 aattggtcac tggagaagat caaagcggca atgcccatgc tgtgctcgtc taatattgtt
     2821 ggcctgcaca agtattggct cgagtttgcc gtctaacaat tcaatcaagc cgatgccgct
     2881 tcgcggcacg gcttatttca ggcgttaggc atcatgggtg aatttttccc tgcacaagtt
     2941 ttcaagcagc tgtcccacgc tcgcgcggtg atcgagcgcc atctggctgc gacactggac
     3001 acaatccacc tgttcggatc tgcgatcgat ggagggctga agccggacag cgacatagac
     3061 ttgctcgtga ccgtcagcgc cgcacctaac gattcgctcc ggcaggcgct aatgctcgat
     3121 ttgctgaaag tctcatcacc gccaggcgat ggcggaacat ggcgaccgct ggagctaact
     3181 gttgtcgctc gaagcgaagt agtgccttgg cgctatccgg cgcggcgtga gcttcagttc
     3241 ggtgagtggc tccgccacga catcctttcc ggaacgttcg agcctgccgt tctggatcac
     3301 gatcttgcga ttttgctgac caaggcgagg caacacagcc ttgcgcttct aggcccatcc
     3361 gcagccacgt ttttcgagcc ggtgccgaag gagcatttct ccaaggcgct tttcgacact
     3421 attgcccagt ggaatgcaga gtcggattgg aagggtgacg agcggaacgt cgttcttgct
     3481 cttgctcgca tttggtacag cgcttcaact ggtctcattg ctcctaagga cgttgctgcc
     3541 gcatgggtat cggagcgttt gcctgccgag catcggcccc tcatctgcaa ggcacgcgcg
     3601 gcgtacctgg gtagcgagga cgacgaccta gcaatgcgcg tcgaagagac ggccgcgttc
     3661 gttcgatatg ccaaagcaac gattgagaga atcttgcgtt gagcggcatg tgcgaaaagt
     3721 gcatcgaccc gcgccgaggg catctgatgc ctaactcggc gttcaagcgg acgggctgcg
     3781 cccgccgctc aattatgcgt tagatgcact aagcacataa ttgctcacag ccaaactatc
     3841 aggtcaagtc tgcttttatt atttttaagc gtgcataata agccctacac aaattgggag
     3901 atatatcatg aaaggctggc tttttcttgt tatcgcaata gttggcgaag taatcgcaac
     3961 atccgcatta aaatctagcg agggctttac taagcttgcc ccttccgccg ttgtcataat
     4021 cggttatggc atcgcatttt attttctttc tctggttctg aaatccatcc ctgtcggtgt
     4081 tgcttatgca gtctggtcgg gactcggcgt cgtcataatt acagccattg cctggttgct
     4141 tcatgggcaa aagcttgatg cgtggggctt tgtaggtatg gggctcataa ttgctgcctt
     4201 tttgctcgcc cgatccccat cgtggaagtc gctgcggagg ccgacgccat ggtgacggtg
     4261 ttcggcattc tgaatctcac cgaggactcc ttcttcgatg agagccggcg gctagacccc
     4321 gccggcgctg tcaccgcggc gatcgaaatg ctgcgagtcg gatcagacgt cgtggatgtc
     4381 ggaccggccg ccagccatcc ggacgcgagg cctgtatcgc cggccgatga gatcagacgt
     4441 attgcgccgc tcttagacgc cctgtccgat cagatgcacc gtgtttcaat cgacagcttc
     4501 caaccggaaa cccagcgcta tgcgctcaag cgcggcgtgg gctacctgaa cgatatccaa
     4561 ggatttcctg accctgcgct ctatcccgat attgctgagg cggactgcag gctggtggtt
     4621 atgcactcag cgcagcggga tggcatcgcc acccgcaccg gtcaccttcg acccgaagac
     4681 gcgctcgacg agattgtgcg gttcttcgag gcgcgggttt ccgccttgcg acggagcggg
     4741 gtcgctgccg accggctcat cctcgatccg gggatgggat ttttcttgag ccccgcaccg
     4801 gaaacatcgc tgcacgtgct gtcgaacctt caaaagctga agtcggcgtt ggggcttccg
     4861 ctattggtct cggtgtcgcg gaaatcctt
//
LOCUS       DAZFQD010000447         4567 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.guided.228, whole genome shotgun sequence.
ACCESSION   DAZFQD010000447 DAZFQD010000000
VERSION     DAZFQD010000447.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4567
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.guided.228"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..630
                     /locus_tag="ACHKTW_005472"
     CDS             <1..630
                     /locus_tag="ACHKTW_005472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HFO6466285.1"
                     /translation="QHLQLLVHSGSRGVGQSILQRHIASFSHHGLPQGSDDALAFACI
                     NRQMIALRIMQQVKATGSPVLDVAHNFVSACQIGDQQGWLHRKGATPDDNGLVIIPGS
                     RGDYSWIVQPVANEKTLHSLAHGAGRKWGRTECKGRLAAKYTATQLSRTELGSRVICR
                     DKQLIFEEAPQAYKSAESVVQCLVQAGLIIPVARLRPVLTLKNSGGKKG"
     gene            627..1241
                     /gene="prfH"
                     /locus_tag="ACHKTW_005473"
     CDS             627..1241
                     /gene="prfH"
                     /locus_tag="ACHKTW_005473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HFO6466286.1"
                     /translation="MILLQLSSAQGPEECCLAVKKALDRLIKEAARQDVAVTVLETET
                     GHYSDTLRSALISLDGDNAWALSESWCGTIQWICPSPYRPHHGRKNWFLGIGRFTADE
                     QEQSDAIRYETLRSSGPGGQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLL
                     IAWKLEQQQQENSAVLKSQRRMFHHQIERGNPRRTFTGMAFIEG"
     gene            complement(1298..2755)
                     /gene="pepD"
                     /locus_tag="ACHKTW_005474"
     CDS             complement(1298..2755)
                     /gene="pepD"
                     /locus_tag="ACHKTW_005474"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HFO6466287.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDVL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            3016..3474
                     /gene="gpt"
                     /locus_tag="ACHKTW_005475"
     CDS             3016..3474
                     /gene="gpt"
                     /locus_tag="ACHKTW_005475"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFO6466288.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            3566..>4567
                     /gene="frsA"
                     /locus_tag="ACHKTW_005476"
     CDS             3566..>4567
                     /gene="frsA"
                     /locus_tag="ACHKTW_005476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HFO6466289.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDDA
                     "
BASE COUNT         1067 a         1148 c         1239 g         1113 t
ORIGIN      
        1 caacatctgc aactgttggt tcatagcggc tcgcgggggg tggggcagtc tattttacag
       61 cggcatattg cctcgttctc gcatcatggt ttgcctcaag gtagtgacga cgcgctggcg
      121 tttgcgtgta ttaatcgcca gatgatcgct ttgcgcataa tgcaacaagt taaggccacg
      181 ggaagtccgg ttctggatgt ggcgcataac tttgttagcg cgtgtcaaat cggcgatcaa
      241 cagggctggt tgcatcgtaa aggtgccacg ccggatgaca acggtctggt gattattccc
      301 ggttcacgtg gtgattactc ctggattgta cagcctgttg cgaacgaaaa aacgctccac
      361 tcgctggcgc atggggcagg gcgtaaatgg gggcgcaccg agtgtaaagg gcgtctggca
      421 gcgaaataca cggcgacgca actctcgcgt actgaacttg gcagtcgggt aatttgtcgc
      481 gataaacaac tcatctttga agaagcgcca caagcctata aatcggctga aagcgtggtg
      541 caatgtctgg tgcaggccgg tttaattatt cctgtcgcgc ggctgcgtcc ggtgctaacg
      601 ctcaaaaaca gtggagggaa aaaaggatga tcttgctaca actctcctca gcgcaggggc
      661 cggaagaatg ttgtctcgca gtgaaaaagg cgctggacag gctaattaaa gaagctgccc
      721 gacaagacgt cgcggtaacg gtgctggaaa cagaaacggg ccactactct gacacgctgc
      781 gttcggcgct gatttctctg gatggcgata acgcatgggc attaagcgaa agctggtgtg
      841 gcactattca gtggatttgt ccgagtccgt atcggcctca tcatgggcgc aaaaactggt
      901 ttctgggcat tgggcgtttt accgctgatg agcaggaaca atcggatgca atccgttatg
      961 agacgttgcg ttcgtcgggg ccgggcggtc aacatgtcaa taaaaccgac tcggcggtac
     1021 gcgccacgca tttggcatcc ggtattagcg tgaaggttca gtcagagcgt agtcagcatg
     1081 ctaacaagcg gctggcacga ttgctgattg cctggaagct ggagcagcag caacaggaaa
     1141 atagcgcggt gctgaaatcg cagcggcgaa tgttccatca ccagattgaa cgtggcaacc
     1201 cgagacggac atttacaggg atggctttta tcgaaggata atgaaggatg aaactgccgg
     1261 aaggcgatta aacgccatcc ggcagcaaat caaataatta cttcgccgga atttctttca
     1321 gcagttcagt cagcagtgtc cagtaatgac ctacgctttc gatgtgaact tgctcatccg
     1381 gagagtgtgg accggtgata gttggcccga tagaaaccat gtccatttcc ggatacggtt
     1441 ttttgaacag accacattcc aggcccgcgt ggataatctg gatgttcggc gttttgttga
     1501 acagacgctg ataggtttca cgtaccagat gcatcaccgg agaattagcg tccggctgcc
     1561 agccaggata tgcgcctttc gcttcggttt tcgcgccagc cagtttaccc agcgaatcca
     1621 gcatgctcac cacgtagtct ttaccgctgt cgatcagtga acggatcagg cagtgaattt
     1681 ctacgttatt gtcagtcatg gtcaccacac cgacgttcag ggaggtttcg accacacctt
     1741 ttgccacgtc ggaattgcgg atcacaccgt tcggggtggc gttcagcaga cgaataaagg
     1801 tatcgcgaga tttcgcaatc agggcagctt tatcgttcgc tacagagtcc agcaacaagg
     1861 ccagattttt ctctttctct gccagctcgt ttttcaggat ctcctgatag gtattcacca
     1921 gagatttcag gacgtcgact ttatcagctg cgacagcaat ggtcgcaaag gcttcacgcg
     1981 ggatggcgtt acgcagtgtg ccgccgttga aatcgataag gcgcagatcc agttcttccg
     2041 catgacccgc caggaagcgc accagtagtt tgttggcatt acccaggcca acgtggattt
     2101 caccgccgga gtgaccgcct ttcagacctt ttaaggttaa cttgaaggtt tcaaaaccag
     2161 ctggaaccgc ttcacgatct aaatgcaggt tggaggtgaa gtcgataccc cccgcacaac
     2221 ccatgtagat ttcaccttct tcttcggagt cggtgttaat cagaatatca gcctgcaacc
     2281 agttgctctg taagccgaac gcaccgtcca taccggcttc ttcggtcatg gtcagcagca
     2341 cttccagcgg gccgtgaacc acgttttcgt cagccagaac cgccagcgca gacgccatac
     2401 caatgccgtt atctgcaccc agcgtggtgc cgcgcgcttt aacccattcg ccatcaatat
     2461 aaggctggat aggatctttc gtgaagtcat gcacggtgtc gttatttttc tgcggcacca
     2521 tatcgaggtg ggcctgtaag acgaccggtt tacgattttc catacctgcg gtagcaggtt
     2581 tacgaatcag gatattacct acctgatcgc gttcgacatg gaaacctttc tcttttgccc
     2641 aaccaacaat gtattcagcg agttgctctt catgatagga cgggtgagga atagaacaga
     2701 ttttggcaaa aatatcccac agcggctgtg gagataattg agacagttca gacacgttaa
     2761 gtctccttgt cgatcacccg caaaacagta ttgcaggtca cagggttagc agaaaatgtt
     2821 gtcaacacaa gacaggcttg cgagatatgt ttgagaatac cactttatcc cgcgtcaggg
     2881 agaggcagtg cgtaaaaaga cgcggactca tgtgaaatac tggtttttag tgcgccagat
     2941 ctctataatc tcgcgcaacc tattttcccc tcgaacactt tttaagccgt agataaacag
     3001 gctgggacac ttcacatgag cgaaaaatac atcgtcacct gggacatgtt gcagatccat
     3061 gcacgtaaac tcgcaagccg actgatgcct tctgaacaat ggaaaggcat tattgccgta
     3121 agccgtggcg gtctggtacc gggtgcgtta ctggcgcgtg aactgggtat tcgtcatgtc
     3181 gataccgttt gtatttccag ctacgatcac gacaaccagc gcgagcttaa agtgctaaaa
     3241 cgcgcagaag gcgatggcga aggcttcatc gttattgatg acctggtgga taccggtggt
     3301 actgcggttg cgattcgtga aatgtatcca aaagcacatt ttgtgactat cttcgcaaaa
     3361 ccggctggtc gtccgctggt tgatgactat gttgttgata tcccgcaaga tacctggatc
     3421 gaacagccgt gggatatggg cgtcgtattc gtcccgccaa tctccggtcg ctaatctttt
     3481 caacgcctgg cactgccggg cgttgttctt tttaacttca ggcgggttac aatagtttcc
     3541 agtaagtatt ctggaggctg catccatgac acaggcaaac ctgagcgaaa ccctgttcaa
     3601 accccgcttt aaacatcctg aaacctcgac gctagtccgc cgctttaatc acggcgcaca
     3661 accgcctgtg cagtcggctc ttgatggtaa aaccatccct cactggtatc gcatgattaa
     3721 ccgtctgatg tggatctggc gcggcattga cccacgtgaa atcctcgacg tccaggcacg
     3781 tattgtgatg agcgatgctg aacgtaccga tgatgattta tacgataccg tgattggcta
     3841 ccgtggtggc aactggattt atgagtgggc cacccaggcg atggtgtggc aacaaaaagc
     3901 ctgtgcggaa gaagatccgc aactcagtgg tcgacactgg ctgcatgcgg ctacgttgta
     3961 caacattgcc gcctatcctc atctgaaagg agatgacctg gccgagcaag cgcaggcttt
     4021 atcaaaccgc gcctatgaag aggccgccca gcgtctgccg ggcacgatgc ggcagatgga
     4081 gtttaccgta cccggcggtg cgcccatcac cggctttttg catatgccga aaggcgatgg
     4141 cccgttcccg acagtattaa tgtgtggtgg tctggatgcg atgcagacgg actattacag
     4201 cctgtatgaa cgttattttg cgccgcgcgg cattgcgatg ctgactattg atatgccgtc
     4261 ggtgggcttt tcttcaaaat ggaagctcac ccaggactcc agcctgttgc atcagcacgt
     4321 cttaaaggcg ctgcctaacg taccgtgggt ggatcacact cgcgtcgcgg cctttggttt
     4381 ccgtttcggc gctaacgttg ccgtgcgtct ggcatacctt gaatcgccgc gtctgaaagc
     4441 ggttgcctgt cttggtccgg tagttcatac cctgttgagt gattttaagt gccagcaaca
     4501 ggtgccggaa atgtatcttg acgttctggc gagtcgtttg gggatgcatg atgcttccga
     4561 tgacgcg
//
LOCUS       DAZFQD010000448         3169 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.guided.256, whole genome shotgun sequence.
ACCESSION   DAZFQD010000448 DAZFQD010000000
VERSION     DAZFQD010000448.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3169
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.guided.256"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            3..719
                     /gene="frdB"
                     /locus_tag="ACHKTW_005477"
     CDS             3..719
                     /gene="frdB"
                     /locus_tag="ACHKTW_005477"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007678861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HFO6466290.1"
                     /translation="MKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYIKDNLAP
                     DLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERDLVVDMT
                     HFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACPQFGLNP
                     EFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKHVDPAAA
                     IQQGKVESSKDFLIATLKPR"
     gene            730..1125
                     /gene="frdC"
                     /locus_tag="ACHKTW_005478"
     CDS             730..1125
                     /gene="frdC"
                     /locus_tag="ACHKTW_005478"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HFO6466291.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            1136..1495
                     /gene="frdD"
                     /locus_tag="ACHKTW_005479"
     CDS             1136..1495
                     /gene="frdD"
                     /locus_tag="ACHKTW_005479"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HFO6466292.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGIVTI"
     gene            1558..2691
                     /locus_tag="ACHKTW_005480"
     CDS             1558..2691
                     /locus_tag="ACHKTW_005480"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HFO6466293.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPTTKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPAEEKNYAWG
                     YREGKAVHVSPGALDAKTYGVKSTIEDMACWVRSNMNPRDINDKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSGNKIALAAHPVKAITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAAWQILNALQ"
     gene            2780..>3169
                     /gene="blc"
                     /locus_tag="ACHKTW_005481"
     CDS             2780..>3169
                     /gene="blc"
                     /locus_tag="ACHKTW_005481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HFO6466294.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALV"
BASE COUNT          777 a          813 c          818 g          761 t
ORIGIN      
        1 acctgaaaat tgaggtggtg cgctataacc cggaagtcga taccgcaccg catagcgcat
       61 tctatgaagt gccttatgac gcaactacct cattactgga tgcgctgggc tacatcaaag
      121 acaacctggc accggacctg agttaccgct ggtcctgccg tatggcgatt tgtggttcct
      181 gcggcatgat ggttaacaac gtgccaaaac tggcatgtaa aaccttcctg cgtgattaca
      241 ccgacggtat gaaggttgaa gcgttagcta acttcccgat tgaacgcgat ctggtggtcg
      301 atatgaccca cttcatcgaa agtctggaag cgatcaaacc gtacatcatc ggcaactccc
      361 gcaccgcgga tcagggtact aacatccaga ccccggcgca gatggcaaag tatcaccagt
      421 tctccggttg catcaactgt ggtctgtgct acgccgcgtg cccgcagttt ggcctgaacc
      481 cagagttcat cggtccggct gccattacgc tggcgcatcg ttataacgaa gatagccgcg
      541 accacggtaa gaaggagcgt atggcgcagt tgaacagcca gaacggcgta tggagctgta
      601 ctttcgtggg ctattgctcc gaagtctgcc cgaaacacgt cgatccggct gcggccattc
      661 agcagggcaa agtagaaagt tcgaaagact ttcttatcgc gaccctgaaa ccacgctaag
      721 gagtgcaaca tgacgactaa acgtaaaccg tatgtacggc caatgacgtc cacctggtgg
      781 aaaaaattgc cgttttatcg cttttacatg ctgcgcgaag gcacggcggt tccggctgtg
      841 tggttcagca ttgaactgat tttcgggctg tttgccctga aaaatggccc ggaagcctgg
      901 gcgggattcg tcgacttttt acaaaacccg gttatcgtga tcattaacct gatcactctg
      961 gcggcagctc tgctgcacac caaaacctgg tttgaactgg caccgaaagc ggccaatatc
     1021 attgtaaaag acgaaaaaat gggaccagag ccaattatca aaagtctctg ggcggtaact
     1081 gtggttgcca ccatcgtaat cctgtttgtt gccctgtact ggtaaggagc ctgagatgat
     1141 taatccaaat ccaaagcgtt ctgacgaacc ggtattctgg ggcctcttcg gggccggtgg
     1201 tatgtggagc gcaattattg cgccggtgat gatcctgctg gtgggtattc tgctgccact
     1261 ggggctgttt ccgggtgatg cgctgagcta cgagcgcgtt ctggcgttcg cgcagagctt
     1321 cattggtcgc gtattcctgt tcctgatgat cgttctgccg ctgtggtgtg gtttacaccg
     1381 tatgcaccac gcgatgcacg atctgaaaat tcacgtgcct gcgggcaaat gggttttcta
     1441 cggtctggct gctatcctga cagttgtcac gctgattggt atcgttacaa tctaacgtat
     1501 cgccaatgta aatccggccc gcctatggcg ggccgttttg tatggaaacc agaccttatg
     1561 ttcaaaacga cgctctgcgc cttattaatt accgcctctt gctccacatt tgctgcccct
     1621 caacaaatca acgatattgt gcatcgcaca attaccccgc ttatagagca acaaaagatc
     1681 cccggtatgg cggtggcggt aatttatcag ggtaaacctt attactttac ctggggctat
     1741 gcggacatcg ccaaaaagca gcccgtcaca cagcaaacgt tgtttgagtt aggttcggtc
     1801 agcaaaacat ttacgggcgt gcttggtggc gacgctattg ctcgagggga aatcaagtta
     1861 agcgatccca caacaaaata ctggcctgaa cttaccgcta aacagtggaa tgggatcaca
     1921 ctattacatc tcgcaaccta cactgctggc ggcctgccat tgcaggtgcc ggatgaggtg
     1981 aaatcctcaa gcgacttgct gcgcttctat caaaactggc agcctgcatg ggcgccagga
     2041 acacaacgtc tgtatgccaa ctccagtatc ggtttgttcg gcgcactggc tgtgaagccg
     2101 tctggtttga gttttgagca ggcgatgcaa acacgtgtct tccagccact caaactcaac
     2161 catacgtgga ttaatgtacc gcccgcagaa gaaaagaatt acgcctgggg atatcgcgaa
     2221 ggcaaggcag ttcatgtttc gccaggggcg ttagatgcta aaacttatgg tgtgaagtcg
     2281 accattgaag atatggcctg ctgggtacga agcaatatga atccccgtga tatcaacgac
     2341 aaaacacttc agcaagggat acaactggca caatctcgct actggcaaac cggcgatatg
     2401 tatcagggcc tgggctggga aatgctggac tggccggtaa atcctgacag catcattaac
     2461 ggcagtggca ataaaattgc actggcagca caccccgtaa aagcgattac gcccccaact
     2521 cctgcagtac gcgcatcatg ggtacataaa acaggggcaa ccggcggatt tggtagctat
     2581 gtcgcgttta ttccagaaaa agagctgggt atagtgatgc tggctaacaa aaactatccc
     2641 aatccagcga gagtcgccgc cgcctggcag attctcaacg ctctacagta aaatttcatc
     2701 gggtccgaat tttcggacct tttctgcgct tttccttgct gtcatctaca cttagaaaaa
     2761 aaccagtaag gaaacattta tgcgcctgct ccctctcgtt gccgcagcga cagctgcatt
     2821 tctggtcgtt gcctgcagtt ctcctacgcc gccgcgtggc gtaacagtag tgaataattt
     2881 cgacgccaaa cgttatctcg gtacctggta tgagattgcc cgttttgatc accgttttga
     2941 acgtggactg gaaaaagtca ccgcaacata cagcctgcgt gatgacggcg gcctgaatgt
     3001 cattaataaa ggctataacc ctgacagagg aatgtggcag cagagtgaag ggaaagcgta
     3061 ctttaccggc gcaccaactc gcgctgcgct gaaagtgtca ttctttggtc ctttctatgg
     3121 cggttataac gttattgcac tcgatcggga ataccgccat gcgctggtt
//
LOCUS       DAZFQD010000449         2449 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.guided.279, whole genome shotgun sequence.
ACCESSION   DAZFQD010000449 DAZFQD010000000
VERSION     DAZFQD010000449.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2449
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.guided.279"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(168..818)
                     /gene="tetR(A)"
                     /locus_tag="ACHKTW_005482"
     CDS             complement(168..818)
                     /gene="tetR(A)"
                     /locus_tag="ACHKTW_005482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(A)"
                     /protein_id="HFO6466295.1"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWH
                     FRNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAG
                     TRPGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGG
                     TVEQAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     gene            924..2123
                     /gene="tet(A)"
                     /locus_tag="ACHKTW_005483"
     CDS             924..2123
                     /gene="tet(A)"
                     /locus_tag="ACHKTW_005483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008493 - tetracycline transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015904 - tetracycline transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(A)"
                     /protein_id="HFO6466296.1"
                     /translation="MKPNRPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAH
                     YGILLALYALMQFACAPVLGALSDRFGRRPVLLVSLAGAAVDYAIMATAPFLWVLYIG
                     RIVAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPH
                     APFFAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAV
                     FFIMQLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGE
                     RRALMLGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQG
                     QLQGSLAALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSG
                     AGQRADR"
     gene            complement(2155..>2449)
                     /locus_tag="ACHKTW_005484"
     CDS             complement(2155..>2449)
                     /locus_tag="ACHKTW_005484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFO6466297.1"
                     /translation="IPMPTGTNVLGLAWLGLIGAGLTYFLWFRGISRLEPTVVSLLGF
                     LSPGTAVLLGWLFLDQTLSALQIIGVLLVIGSIWLGQRSNRTPRARIACRKSP"
BASE COUNT          419 a          765 c          767 g          498 t
ORIGIN      
        1 ggccgccaga gaggccgagc gcggccgtga ggcttggacg ctagggcagg gcatgaaaaa
       61 gcccgtagcg ggctgctacg ggcgtctgac gcggtggaaa gggggagggg atgttgtcta
      121 catggctctg ctgtagtgag tgggttgcgc tccggcagcg gtcctgatca atcgtcaccc
      181 tttctcggtc cttcaacgtt cctgacaacg agcctccttt tcgccaatcc atcgacaatc
      241 accgcgagtc cctgctcgaa cgctgcgtcc ggaccggctt cgtcgaaggc gtctatcgcg
      301 gcccgcaaca gcggcgagag cggagcctgt tcaacggtgc cgccgcgctc gccggcatcg
      361 ctgtcgccgg cctgctcctc aagcacggcc ccaacagtga agtagctgat tgtcatcagc
      421 gcattgacgg cgtccccggc cgaaaaaccc gcctcgcaga ggaagcgaag ctgcgcgtcg
      481 gccgtttcca tctgcggtgc gcccggtcgc gtgccggcat ggatgcgcgc gccatcgcgg
      541 taggcgagca gcgcctgcct gaagctgcgg gcattcccga tcagaaatga gcgccagtcg
      601 tcgtcggctc tcggcaccga atgcgtatga ttctccgcca gcatggcttc ggccagtgcg
      661 tcgagcagcg cccgcttgtt cctgaagtgc cagtaaagcg ccggctgctg aacccccaac
      721 cgttccgcca gtttgcgtgt cgtcagaccg tctacgccga cctcgttcaa caggtccagg
      781 gcggcacgga tcactgtatt cggctgcaac tttgtcatgc ttgacacttt atcactgata
      841 aacataatat gtccaccaac ttatcagtga taaagaatcc gcgcgttcaa tcggaccagc
      901 ggaggctggt ccggaggcca gacgtgaaac ccaacagacc cctgatcgta attctgagca
      961 ctgtcgcgct cgacgctgtc ggcatcggcc tgattatgcc ggtgctgccg ggcctcctgc
     1021 gcgatctggt tcactcgaac gacgtcaccg cccactatgg cattctgctg gcgctgtatg
     1081 cgttgatgca atttgcctgc gcacctgtgc tgggcgcgct gtcggatcgt ttcgggcggc
     1141 ggccggtctt gctcgtctcg ctggccggcg ctgctgtcga ctacgccatc atggcgacgg
     1201 cgcctttcct ttgggttctc tatatcgggc ggatcgtggc cggcatcacc ggggcgactg
     1261 gggcggtagc cggcgcgtat attgccgata tcactgatgg cgatgagcgc gcgcggcact
     1321 tcggcttcat gagcgcctgt ttcgggttcg ggatggtcgc gggacctgtg ctcggtgggc
     1381 tgatgggcgg tttctccccc cacgctccgt tcttcgccgc ggcagccttg aacggcctca
     1441 atttcctgac gggctgtttc cttttgccgg agtcgcacaa aggcgaacgc cggccgttac
     1501 gccgggaggc tctcaacccg ctcgcttcgt tccggtgggc ccggggcatg accgtcgtcg
     1561 ccgccctgat ggcggtcttc ttcatcatgc aacttgtcgg acaggtgccg gccgcgcttt
     1621 gggtcatttt cggcgaggat cgctttcact gggacgcgac cacgatcggc atttcgcttg
     1681 ccgcatttgg cattctgcat tcactcgccc aggcaatgat caccggccct gtagccgccc
     1741 ggctcggcga aaggcgggca ctcatgctcg gaatgattgc cgacggcaca ggctacatcc
     1801 tgcttgcctt cgcgacacgg ggatggatgg cgttcccgat catggtcctg cttgcttcgg
     1861 gtggcatcgg aatgccggcg ctgcaagcaa tgttgtccag gcaggtggat gaggaacgtc
     1921 aggggcagct gcaaggctca ctggcggcgc tcaccagcct gacctcgatc gtcggacccc
     1981 tcctcttcac ggcgatctat gcggcttcta taacaacgtg gaacgggtgg gcatggattg
     2041 caggcgctgc cctctacttg ctctgcctgc cggcgctgcg tcgcgggctt tggagcggcg
     2101 cagggcaacg agccgatcgc tgatcgtgga aacgataggc ctatgccatg cgggtcaagg
     2161 cgacttccgg caagctatac gcgccctagg agtgcggttg gaacgttggc ccagccagat
     2221 actcccgatc acgagcagga cgccgatgat ttgaagcgca ctcagcgtct gatccaagaa
     2281 caaccatcct agcaacacgg cggtccccgg gctgagaaag cccagtaagg aaacaactgt
     2341 aggttcgagt cgcgagatcc cccggaacca aaggaagtag gttaaacccg ctccgatcag
     2401 gccgagccac gccaggccga gaacattggt tcctgtaggc atcgggatt
//
LOCUS       DAZFQD010000450         2449 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.guided.280, whole genome shotgun sequence.
ACCESSION   DAZFQD010000450 DAZFQD010000000
VERSION     DAZFQD010000450.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2449
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.guided.280"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(168..818)
                     /gene="tetR(A)"
                     /locus_tag="ACHKTW_005485"
     CDS             complement(168..818)
                     /gene="tetR(A)"
                     /locus_tag="ACHKTW_005485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(A)"
                     /protein_id="HFO6466298.1"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWH
                     FRNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAG
                     TRPGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGG
                     TVEQAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     gene            924..2123
                     /gene="tet(A)"
                     /locus_tag="ACHKTW_005486"
     CDS             924..2123
                     /gene="tet(A)"
                     /locus_tag="ACHKTW_005486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008493 - tetracycline transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015904 - tetracycline transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(A)"
                     /protein_id="HFO6466299.1"
                     /translation="MKPNRPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAH
                     YGILLALYALMQFACAPVLGALSDRFGRRPVLLVSLAGAAVDYAIMATAPFLWVLYIG
                     RIVAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPH
                     APFFAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAV
                     FFIMQLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGE
                     RRALMLGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQG
                     QLQGSLAALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSG
                     AGQRADR"
     gene            complement(2155..>2449)
                     /locus_tag="ACHKTW_005487"
     CDS             complement(2155..>2449)
                     /locus_tag="ACHKTW_005487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFO6466300.1"
                     /translation="IPMPTGTNVLGLAWLGLIGAGLTYFLWFRGISRLEPTVVSLLGF
                     LSPGTAVLLGWLFLDQTLSALQIIGVLLVIGSIWLGQRSNRTPRARIACRKSP"
BASE COUNT          419 a          765 c          766 g          499 t
ORIGIN      
        1 ggccgccaga gaggccgagc gcggccgtga ggcttggacg ctagggcagg gcatgaaaaa
       61 gcccgtagcg ggctgctacg ggcgtctgac gcggtggaaa gggggagggg atgttgtcta
      121 catggctctg ctgtagtgag tgggttgcgc tccggcagcg gtcctgatca atcgtcaccc
      181 tttctcggtc cttcaacgtt cctgacaacg agcctccttt tcgccaatcc atcgacaatc
      241 accgcgagtc cctgctcgaa cgctgcgtcc ggaccggctt cgtcgaaggc gtctatcgcg
      301 gcccgcaaca gcggcgagag cggagcctgt tcaacggtgc cgccgcgctc gccggcatcg
      361 ctgtcgccgg cctgctcctc aagcacggcc ccaacagtga agtagctgat tgtcatcagc
      421 gcattgacgg cgtccccggc cgaaaaaccc gcctcgcaga ggaagcgaag ctgcgcgtcg
      481 gccgtttcca tctgcggtgc gcccggtcgc gtgccggcat ggatgcgcgc gccatcgcgg
      541 taggcgagca gcgcctgcct gaagctgcgg gcattcccga tcagaaatga gcgccagtcg
      601 tcgtcggctc tcggcaccga atgcgtatga ttctccgcca gcatggcttc ggccagtgcg
      661 tcgagcagcg cccgcttgtt cctgaagtgc cagtaaagcg ccggctgctg aacccccaac
      721 cgttccgcca gtttgcgtgt cgtcagaccg tctacgccga cctcgttcaa caggtccagg
      781 gcggcacgga tcactgtatt cggctgcaac tttgtcatgc ttgacacttt atcactgata
      841 aacataatat gtccaccaac ttatcagtga taaagaatcc gcgcgttcaa tcggaccagc
      901 ggaggctggt ccggaggcca gacgtgaaac ccaacagacc cctgatcgta attctgagca
      961 ctgtcgcgct cgacgctgtc ggcatcggcc tgattatgcc ggtgctgccg ggcctcctgc
     1021 gcgatctggt tcactcgaac gacgtcaccg cccactatgg cattctgctg gcgctgtatg
     1081 cgttgatgca atttgcctgc gcacctgtgc tgggcgcgct gtcggatcgt ttcgggcggc
     1141 ggccggtctt gctcgtctcg ctggccggcg ctgctgtcga ctacgccatc atggcgacgg
     1201 cgcctttcct ttgggttctc tatatcgggc ggatcgtggc cggcatcacc ggggcgactg
     1261 gggcggtagc cggcgcttat attgccgata tcactgatgg cgatgagcgc gcgcggcact
     1321 tcggcttcat gagcgcctgt ttcgggttcg ggatggtcgc gggacctgtg ctcggtgggc
     1381 tgatgggcgg tttctccccc cacgctccgt tcttcgccgc ggcagccttg aacggcctca
     1441 atttcctgac gggctgtttc cttttgccgg agtcgcacaa aggcgaacgc cggccgttac
     1501 gccgggaggc tctcaacccg ctcgcttcgt tccggtgggc ccggggcatg accgtcgtcg
     1561 ccgccctgat ggcggtcttc ttcatcatgc aacttgtcgg acaggtgccg gccgcgcttt
     1621 gggtcatttt cggcgaggat cgctttcact gggacgcgac cacgatcggc atttcgcttg
     1681 ccgcatttgg cattctgcat tcactcgccc aggcaatgat caccggccct gtagccgccc
     1741 ggctcggcga aaggcgggca ctcatgctcg gaatgattgc cgacggcaca ggctacatcc
     1801 tgcttgcctt cgcgacacgg ggatggatgg cgttcccgat catggtcctg cttgcttcgg
     1861 gtggcatcgg aatgccggcg ctgcaagcaa tgttgtccag gcaggtggat gaggaacgtc
     1921 aggggcagct gcaaggctca ctggcggcgc tcaccagcct gacctcgatc gtcggacccc
     1981 tcctcttcac ggcgatctat gcggcttcta taacaacgtg gaacgggtgg gcatggattg
     2041 caggcgctgc cctctacttg ctctgcctgc cggcgctgcg tcgcgggctt tggagcggcg
     2101 cagggcaacg agccgatcgc tgatcgtgga aacgataggc ctatgccatg cgggtcaagg
     2161 cgacttccgg caagctatac gcgccctagg agtgcggttg gaacgttggc ccagccagat
     2221 actcccgatc acgagcagga cgccgatgat ttgaagcgca ctcagcgtct gatccaagaa
     2281 caaccatcct agcaacacgg cggtccccgg gctgagaaag cccagtaagg aaacaactgt
     2341 aggttcgagt cgcgagatcc cccggaacca aaggaagtag gttaaacccg ctccgatcag
     2401 gccgagccac gccaggccga gaacattggt tcctgtaggc atcgggatt
//
LOCUS       DAZFQD010000451         1804 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.guided.302, whole genome shotgun sequence.
ACCESSION   DAZFQD010000451 DAZFQD010000000
VERSION     DAZFQD010000451.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1804
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.guided.302"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            250..597
                     /locus_tag="ACHKTW_005488"
     CDS             250..597
                     /locus_tag="ACHKTW_005488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter QacE delta 1"
                     /protein_id="HFO6466301.1"
                     /translation="MKGWLFLVIAIVGEVIATSALKSSEGFTKLAPSAVVIIGYGIAF
                     YFLSLVLKSIPVGVAYAVWSGLGVVIITAIAWLLHGQKLDAWGFVGMGLIIAAFLLAR
                     SPSWKSLRRPTPW"
     gene            591..1430
                     /gene="sul1"
                     /locus_tag="ACHKTW_005489"
     CDS             591..1430
                     /gene="sul1"
                     /locus_tag="ACHKTW_005489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul1"
                     /protein_id="HFO6466302.1"
                     /translation="MVTVFGILNLTEDSFFDESRRLDPAGAVTAAIEMLRVGSDVVDV
                     GPAASHPDARPVSPADEIRRIAPLLDALSDQMHRVSIDSFQPETQRYALKRGVGYLND
                     IQGFPDPALYPDIAEADCRLVVMHSAQRDGIATRTGHLRPEDALDEIVRFFEARVSAL
                     RRSGVAADRLILDPGMGFFLSPAPETSLHVLSNLQKLKSALGLPLLVSVSRKSFLGAT
                     VGLPVKDLGPASLAAELHAIGNGADYVRTHAPGDLRSAITFSETLAKFRSRDARDRGL
                     DHA"
     gene            1558..>1804
                     /locus_tag="ACHKTW_005490"
     CDS             1558..>1804
                     /locus_tag="ACHKTW_005490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO6466303.1"
                     /translation="MDSEEPPNVRVACSGDIDEVVRLMHDAAAWMSAKGTPAWDVARI
                     DRTFAETFVLRSELLVASCSDGIVGCCTLSAEDPEFWP"
BASE COUNT          342 a          522 c          534 g          406 t
ORIGIN      
        1 tcgttcgata tgccaaagca acgattgaga gaatcttgcg ttgagcggca tgtgcgaaaa
       61 gtgcatcgac ccgcgccgag ggcatctgat gcctaactcg gcgttcaagc ggacgggctg
      121 cgcccgccgc tcaattatgc gttagatgca ctaagcacat aattgctcac agccaaacta
      181 tcaggtcaag tctgctttta ttatttttaa gcgtgcataa taagccctac acaaattggg
      241 agatatatca tgaaaggctg gctttttctt gttatcgcaa tagttggcga agtaatcgca
      301 acatccgcat taaaatctag cgagggcttt actaagcttg ccccttccgc cgttgtcata
      361 atcggttatg gcatcgcatt ttattttctt tctctggttc tgaaatccat ccctgtcggt
      421 gttgcttatg cagtctggtc gggactcggc gtcgtcataa ttacagccat tgcctggttg
      481 cttcatgggc aaaagcttga tgcgtggggc tttgtaggta tggggctcat aattgctgcc
      541 tttttgctcg cccgatcccc atcgtggaag tcgctgcgga ggccgacgcc atggtgacgg
      601 tgttcggcat tctgaatctc accgaggact ccttcttcga tgagagccgg cggctagacc
      661 ccgccggcgc tgtcaccgcg gcgatcgaaa tgctgcgagt cggatcagac gtcgtggatg
      721 tcggaccggc cgccagccat ccggacgcga ggcctgtatc gccggccgat gagatcagac
      781 gtattgcgcc gctcttagac gccctgtccg atcagatgca ccgtgtttca atcgacagct
      841 tccaaccgga aacccagcgc tatgcgctca agcgcggcgt gggctacctg aacgatatcc
      901 aaggatttcc tgaccctgcg ctctatcccg atattgctga ggcggactgc aggctggtgg
      961 ttatgcactc agcgcagcgg gatggcatcg ccacccgcac cggtcacctt cgacccgaag
     1021 acgcgctcga cgagattgtg cggttcttcg aggcgcgggt ttccgccttg cgacggagcg
     1081 gggtcgctgc cgaccggctc atcctcgatc cggggatggg atttttcttg agccccgcac
     1141 cggaaacatc gctgcacgtg ctgtcgaacc ttcaaaagct gaagtcggcg ttggggcttc
     1201 cgctattggt ctcggtgtcg cggaaatcct tcttgggcgc caccgttggc cttcctgtaa
     1261 aggatctggg tccagcgagc cttgcggcgg aacttcacgc gatcggcaat ggcgctgact
     1321 acgtccgcac ccacgcgcct ggagatctgc gaagcgcaat caccttctcg gaaaccctcg
     1381 cgaaatttcg cagtcgcgac gccagagacc gagggttaga tcatgcctag cattcacctt
     1441 ccggccgccc gctagcggac cctggtcagg ttccgcgaag gtgggcgcag acatgctggg
     1501 ctcgtcagga tcaaactgca ctatgaggcg gcggttcata ccgcgccagg ggagcgaatg
     1561 gacagcgagg agcctccgaa cgttcgggtc gcctgctcgg gtgatatcga cgaggttgtg
     1621 cggctgatgc acgacgctgc ggcgtggatg tccgccaagg gaacgcccgc ctgggacgtc
     1681 gcgcggatcg accggacatt cgcggagacc ttcgtcctga gatccgagct cctagtcgcg
     1741 agttgcagcg acggcatcgt cggctgttgc accttgtcgg ccgaggatcc cgagttctgg
     1801 cccg
//
LOCUS       DAZFQD010000452         1681 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2211T19648
            SAMN43283563-rid23074813.guided.305, whole genome shotgun sequence.
ACCESSION   DAZFQD010000452 DAZFQD010000000
VERSION     DAZFQD010000452.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283563
            Sequence Read Archive: SRR30325518
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351780.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351780.1
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 05:22:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,416
            Genes (coding)                    :: 5,209
            CDSs (with protein)               :: 5,209
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 81 of 207
            Pseudo Genes (incomplete)         :: 109 of 207
            Pseudo Genes (internal stop)      :: 47 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1681
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283563-rid23074813.guided.305"
                     /serotype="O 91:H14"
                     /isolate="2211T19648"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-11"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            308..1255
                     /gene="stxA1a"
                     /locus_tag="ACHKTW_005491"
     CDS             308..1255
                     /gene="stxA1a"
                     /locus_tag="ACHKTW_005491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx1a subunit A"
                     /protein_id="HFO6466304.1"
                     /translation="MKIIIFRVLTFFFVIFSVNVVAKEFTLDFSTAKTYVDSLNVIRS
                     AIGTPLQTISSGGTSLLMIDSGTGDNLFAVDVRGIDPEEGRFNNLRLIVERNNLYVTG
                     FVNRTNNVFYRFADFSHVTFPGTTAVTLSGDSSYTTLQRVAGISRTGMQINRHSLTTS
                     YLDLMSHSGTSLTQSVARAMLRFVTVTAEALRFRQIQRGFRTTLDDLSGRSYVMTAED
                     VDLTLNWGRLSSVLPDYHGQDSVRVGRISFGSINAILGSVALILNCHHHASRVARMAS
                     DEFPSMCPADGRVRGITHNKILWDSSTLGAILMRRTISS"
     gene            1265..1534
                     /gene="stxB1a"
                     /locus_tag="ACHKTW_005492"
     CDS             1265..1534
                     /gene="stxB1a"
                     /locus_tag="ACHKTW_005492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx1a subunit B"
                     /protein_id="HFO6466305.1"
                     /translation="MKKTLLIAASLSFFSASALATPDCVTGKVEYTKYNDDDTFTVKV
                     GDKELFTNRWNLQSLLLSAQITGMTVTIKTNACHNGGGFSEVIFR"
BASE COUNT          451 a          290 c          407 g          533 t
ORIGIN      
        1 caggatgacc ctgtaacgaa gtttgcgtaa cagcattttg ctctacgagt ttgccagccc
       61 ccccagtggc tggctttttt atgtccgtaa catcctgtgt atcaataaat gttgttgtct
      121 acgtacgtca agtagtcgca tgagatctga ccagatatgt taaggttgca gctctctttg
      181 aatataatta tcattttcat tacgttattg ttacgtttat ccggtgcgcc gtaaaacgcc
      241 gtccttcagg gggtggagga tgtcaagaat atagttatcg tatggtgctc aaggagtatt
      301 gtgtaatatg aaaataatta tttttagagt gctaactttt ttctttgtta tcttttcagt
      361 taatgtggtt gcgaaggaat ttaccttaga cttctcgact gcaaagacgt atgtagattc
      421 gctgaatgtc attcgctctg caataggtac tccattacag actatttcat caggaggtac
      481 gtctttactg atgattgata gtggcacagg ggataatttg tttgcagttg atgtcagagg
      541 gatagatcca gaggaagggc ggtttaataa tctacggctt attgttgaac gaaataattt
      601 atatgtgaca ggatttgtta acaggacaaa taatgttttt tatcgctttg ctgatttttc
      661 acatgttacc tttccaggta caacagcggt tacattgtct ggtgacagta gctataccac
      721 gttacagcgt gttgcaggga tcagtcgtac ggggatgcag ataaatcgcc attcgttgac
      781 tacttcttat ctggatttaa tgtcgcatag tggaacctca ctgacgcagt ctgtggcaag
      841 agcgatgtta cggtttgtta ctgtgacagc tgaagcttta cgttttcggc aaatacagag
      901 gggatttcgt acaacactgg atgatctcag tgggcgttct tatgtaatga ctgctgaaga
      961 tgttgatctt acattgaact ggggaaggtt gagtagtgtc ctgcctgact atcatggaca
     1021 agactctgtt cgtgtaggaa gaatttcttt tggaagcatt aatgcaattc tgggaagcgt
     1081 ggcattaata ctgaattgtc atcatcatgc atcgcgagtt gccagaatgg catctgatga
     1141 gtttccttct atgtgtccgg cagatggaag agtccgtggg attacgcaca ataaaatatt
     1201 gtgggattca tccactctgg gggcaattct gatgcgcaga actattagca gttgaggggg
     1261 taaaatgaaa aaaacattat taatagctgc atcgctttca tttttttcag caagtgcgct
     1321 ggcgacgcct gattgtgtaa ctggaaaggt ggagtataca aaatataatg atgacgatac
     1381 ctttacagtt aaagtgggtg ataaagaatt atttaccaac agatggaatc ttcagtctct
     1441 tcttctcagt gcgcaaatta cggggatgac tgtaaccatt aaaactaatg cctgtcataa
     1501 tggaggggga ttcagcgaag ttatttttcg ttgactcaga atagctcagt gaaaatagca
     1561 ggcggagatt cataaatgtt aaatacatct caattcagtc agttgttgcc ggtctgataa
     1621 tagatgtgtt agaaaatttc tgcatggtga atccccctgt gcggaggggc gactggtgaa
     1681 c
//
